title: Yeast two-hybrid approaches to dissecting the plant defense response.
abstract: We describe a reliable GAL4-based yeast two-hybrid system for identifying and isolating clones encoding proteins interacting with a protein of interest. This two-hybrid system gives extremely low background and few false-positive clones, making it ideal for library screening purposes. We have successfully used it not only to isolate Arabidopsis NPR1-interactors from rice but also to pull out the rice NPR1 ortholog using one of the interactors as bait.
authors.affiliation: Department of Plant Pathology, University of California, Davis, USA.
authors.forename: Mawsheng
authors.initials: M
authors.lastname: Chern
authors.forename: Todd
authors.initials: T
authors.lastname: Richter
authors.forename: Pamela C
authors.initials: PC
authors.lastname: Ronald
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D018744:DNA, Plant / Q000302:isolation & purification; D015723:Gene Library; D007113:Immunity, Innate / Q000502:physiology*; D012275:Oryza / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D010935:Plant Diseases; D010957:Plasmids / Q000302:isolation & purification; D014170:Transformation, Genetic; D020798:Two-Hybrid System Techniques; D015003:Yeasts / Q000166:cytology; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D018744:DNA, Plant; D001616:beta-Galactosidase