Parallel manipulation of bifunctional DNA molecules on structured surfaces using kinesin-driven microtubules.
We have developed a technique to manipulate bifunctional DNA molecules: One end is thiolated to bind to a patterned gold surface and the other end is biotinylated to bind to a microtubule gliding over a kinesin-coated surface. We found that DNA molecules can be stretched and overstretched between the gold pads and the motile microtubules, and that they can form dynamic networks. This serves as a proof-of-principle that biological machineries can be used in vitro to accomplish the parallel formation of structured DNA templates that will have applications in biophysics and nanoelectronics.
Dinu
Cerasela Zoica
CZ
Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.
Opitz
Jörg
J
Pompe
Wolfgang
W
Howard
Jonathon
J
Mertig
Michael
M
Diez
Stefan
S
D010582:Bacteriophage lambda / Q000235:genetics; D004279:DNA, Viral / Q000378:metabolism*; D006046:Gold; D016547:Kinesins / Q000502:physiology*; D008870:Microtubules / Q000378:metabolism; D036103:Nanotechnology
D004279:DNA, Viral; D006046:Gold; D016547:Kinesins