title: Novel in vitro screening system for monoclonal antibodies using hypermutating chicken B cell library.
abstract: Here, we report an in vitro screening system for monoclonal antibodies using a hypermutating chicken B cell line, DT40-SW. When switching on hypermutation, cultured DT40-SW cells constituted an antibody library, from which clones secreting antibodies to a test antigen were successfully isolated, and genetically stabilized by switching off the mutation machinery.
authors.affiliation: Department of Biotechnology, Graduate School of Natural Science and Technology, Okayama University, Tsushima-Naka, Okayama 700-8530, Japan.
authors.forename: Kagefumi
authors.initials: K
authors.lastname: Todo
authors.forename: Kenji
authors.initials: K
authors.lastname: Miyake
authors.forename: Masaki
authors.initials: M
authors.lastname: Magari
authors.forename: Naoki
authors.initials: N
authors.lastname: Kanayama
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ohmori
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001402:B-Lymphocytes / Q000378:metabolism*; D002460:Cell Line; D002645:Chickens; D003564:Cytidine Deaminase / Q000502:physiology; D009596:Nitrophenols / Q000276:immunology; D010648:Phenylacetates; D012710:Serum Albumin, Bovine / Q000276:immunology; D027041:Somatic Hypermutation, Immunoglobulin; D000098830:AICDA (Activation-Induced Cytidine Deaminase)
chemical_list: D000911:Antibodies, Monoclonal; D009596:Nitrophenols; D010648:Phenylacetates; C026242:4-hydroxy-5-nitrophenyl acetic acid; D012710:Serum Albumin, Bovine; D000098830:AICDA (Activation-Induced Cytidine Deaminase); D003564:Cytidine Deaminase