Associating hyaluronan derivatives: a novel horizon in viscosupplementation of osteoarthritic joints.
The association of two high-molecular-weight hyaluronan (HA) derivatives, namely a beta-cyclodextrin (HA-beta-CD) and an N-acylurea (EDC-HA), dissolved in aqueous NaCl, was studied. The weight-average of the molecular weights (Mw) of HA-beta-CD and of EDC-HA was 185.3 and 86.8 kDa, respectively. However, the Mw value determined for the equimolar mixture of the two biopolymers equaled 556.0 kDa. Similarly, the radius of gyration (dimension) Rg = 80.6 nm of the above equimolar mixture was significantly greater than the values found for the single macromolecules, i.e., 40.2 nm for HA-beta-CD and 23.8 nm for EDC-HA. These data indicate that the two kinds of substituents, borne by the HA polymeric chains, form host-guest inclusion complexes resulting in polymacromolecular associates/aggregates.
Soltés
Ladislav
L
Institute of Experimental Pharmacology, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84104 Bratislava. exfasolt@savba.sk
Mendichi
Raniero
R
Kogan
Grigorij
G
Mach
Mojmír
M
D000818:Animals; D002417:Cattle; D006820:Hyaluronic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry*; D007270:Injections, Intra-Articular; D007596:Joints / Q000187:drug effects / Q000473:pathology; D010003:Osteoarthritis / Q000188:drug therapy* / Q000473:pathology; D012710:Serum Albumin, Bovine / Q000737:chemistry; D014783:Viscosity
D012710:Serum Albumin, Bovine; D006820:Hyaluronic Acid