[Study on fluoroquinolone resistance and the relationship between resistance and mutations of gyrA and parC in Neisseria gonorrhoeae].
OBJECTIVE
To study the phenotypic and genotypic resistance to Fluoroquinolones in Neisseria gonorrhoeae (NG) isolated in Jiangsu province of China.


METHODS
In-vitro, susceptibility testing of ciprofloxacin and levofloxacin against ninety-five clinical isolates were determined by agar dilution method. Detection of mutation in the gyrA and parC genes was performed by polymerase chain reaction (PCR) assay and sequence analysis.


RESULTS
The clinical isolates demonstrated 100% resistance to ciprofloxacin. Based on gyrA and parC mutations, 18 types could be categorized among the 54 isolates. Based on the same gyrA mutations,isolates with high MIC appeared to have had more mutations in parC gene.


CONCLUSIONS
The status of resistance to ciprofloxacin in NG was quite serious, and ciprofloxacin treatment for the treatment of NG infections in Jiangsu province should not be recommended. The results from this study suggested that mutations in the parC gene had contributed to the development of high Fluoroquinolone resistance in NG.
Xu
Jin-shui
JS
Department of Epidemiology, Public Health School of Southeast University, Nanjing 210009, China.
Wang
Bei
B
Wang
Chang-xian
CX
Gou
Hai-jian
HJ
Mi
Zu-huang
ZH
Ling
Jian-hua
JH
D002681:China; D027081:DNA Gyrase / Q000235:genetics; D027101:DNA Topoisomerase IV / Q000235:genetics; D024881:Drug Resistance, Bacterial; D024841:Fluoroquinolones / Q000494:pharmacology*; D005838:Genotype; D006069:Gonorrhea / Q000188:drug therapy; D006801:Humans; D009344:Neisseria gonorrhoeae / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D010641:Phenotype
D024841:Fluoroquinolones; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase