title: Antioxidant/prooxidant effects of dietary non-flavonoid phenols on the Cu2+-induced oxidation of human low-density lipoprotein (LDL).
abstract: A central role in the oxidative development of atherosclerotic lesions has been ascribed to the peroxidation of plasma low-density lipoprotein (LDL). Dietary supplementation with virgin olive oils increases the total plasma antioxidant status and the resistance of low-density lipoprotein to ex vivo oxidation. We have studied the effects of some dietary non-flavonoid phenols from Olea europaea L., both in purified form or in complex mixtures obtained by biotransformation of olive leaf extracts, on the LDL oxidation induced by Cu2+ ions. Cu2+-Induced LDL oxidation is inhibited by oleuropein and hydroxytyrosol in the initiation phase of the reaction at concentrations of phenols higher than that of Cu2+ ions. Interestingly, at lower concentration, both phenols anticipated the initiation process of LDL oxidation, thus exerting prooxidant capacities. Although similar effects are already described for flavonoids, such as quercetin, rutin, and apigenin, it is the first time that a prooxidant effect of dietary non-flavonoid phenols, such as oleuropein and hydroxytyrosol, on the LDL oxidation is reported. Our results show that a net effect of oleuropein and hydroxytyrosol on Cu2+-induced LDL peroxidation is determined by a balance of their pro- and antioxidant capacities. It is worth to underline that, during Cu2+-induced LDL oxidation in the presence of bioreactor eluates, we have evidence of a synergistic effect among phenolic compounds that enhance their antioxidant capacities so avoiding the prooxidant effects.
authors.affiliation: Institute of Protein Biochemistry, CNR Via Marconi, 10, I-80125 Naples.
authors.forename: Raffaella
authors.initials: R
authors.lastname: Briante
authors.forename: Ferdinando
authors.initials: F
authors.lastname: Febbraio
authors.forename: Roberto
authors.initials: R
authors.lastname: Nucci
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D003300:Copper / Q000494:pharmacology*; D004042:Dietary Fats, Unsaturated / Q000302:isolation & purification / Q000494:pharmacology; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D008077:Lipoproteins, LDL / Q000187:drug effects* / Q000378:metabolism; D031658:Olea; D000069463:Olive Oil; D010084:Oxidation-Reduction / Q000187:drug effects; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves; D010938:Plant Oils / Q000302:isolation & purification / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D004042:Dietary Fats, Unsaturated; D005419:Flavonoids; D008077:Lipoproteins, LDL; D000069463:Olive Oil; D010636:Phenols; D010936:Plant Extracts; D010938:Plant Oils; D017382:Reactive Oxygen Species; D003300:Copper