Protein variability among Mycoplasma hyopneumoniae isolates.
Sodium-dodecyl-sulphate polyacrylamide gel electrophoresis (SDS-PAGE) was used to study the protein variability of Mycoplasma hyopneumoniae isolates. Fifty-six M. hyopneumoniae isolates from 6 different countries and 37 different herds were used. From eight herds, more than one isolate was available. All SDS-PAGE patterns of isolates originating from different herds were clearly divergent. Intra-species protein variability was quantified using the reference strain J and seven field strains all obtained from different herds and classified according to virulence. Between the field strains, a variability of 25% was found, while the culture-adapted strain J was clearly divergent and showed 30% variability with the field strains. No clustering according to virulence was obtained, but a protein band of about 181kDa was present in the two highly virulent isolates whereas this protein band was absent in the moderately and low virulent isolates. Protein patterns of isolates derived from different animals from the same herd, were identical or differed in only a few protein bands. This study clearly indicates that, in agreement with previous studies on genomic diversity of M. hyopneumoniae isolates, proteomic variability within the species is high. Our study did not find clear evidence that more than one M. hyopneumoniae isolate circulates within a herd at a specific time point. The minor differences found between M. hyopneumoniae isolates from the same herd might reflect the organism's ability to alter its proteomic expression profile under field conditions.
Calus
D
D
Laboratory of Veterinary Bacteriology and Mycology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. dries.calus@ugent.be
Baele
M
M
Meyns
T
T
de Kruif
A
A
Butaye
P
P
Decostere
A
A
Haesebrouck
F
F
Maes
D
D
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis*; D016000:Cluster Analysis; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D020869:Gene Expression Profiling / Q000662:veterinary; D005843:Geography; D045705:Mycoplasma hyopneumoniae / Q000737:chemistry* / Q000302:isolation & purification / Q000472:pathogenicity; D045729:Pneumonia of Swine, Mycoplasmal / Q000382:microbiology*; D013552:Swine; D014774:Virulence
D001426:Bacterial Proteins