Identification of nine new HLA class I alleles in volunteers from the Singapore stem cell donor registries.
Based on unusual probe hybridization patterns, two human leukocyte antigen (HLA)-A, five HLA-B, and two HLA-C alleles (A*240208, A*3211Q; B*1822, B*3938Q, B*4606, B*4607N, B*5139; Cw*0321, Cw*0734) were identified in individuals from the Singapore Bone Marrow Donor Program and Singapore Cord Blood Bank. Eight of the nine alleles encode amino acid substitutions altering the antigen-binding region including three alleles with changes altering a cysteine at codon 164 (A*3211Q, B*3938Q, B*4607N). This substitution either eliminates a key disulfide bond or results in a stop codon, both likely affecting the expression of the HLA molecules. Only one allele (A*240208) carries a synonymous substitution.
Tang
T F
TF
Department of Pediatrics, Georgetown University Medical Center, Washington, DC, USA.
Hou
L
L
Tu
B
B
Hwang
W Y K
WY
Yeoh
A E J
AE
Ng
J
J
Hurley
C K
CK
D000483:Alleles; D018070:Biological Specimen Banks; D005091:Exons; D006680:HLA Antigens / Q000235:genetics*; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D012042:Registries; D012846:Singapore; D033581:Stem Cell Transplantation; D014019:Tissue Donors
D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I