Toxin binding to chimeric K+ channels immobilised on a solid nitrocellulose support.
In this work, we used a panel prokaryote/eukaryote K+ channel chimeras to generate K+ channel arrays. Their behaviour in solution was compared with that when spotted on a nitrocellulose-supported film and their responses to selective high affinity ligands, including polypeptide toxins and TEA, were studied.
Legros
Christian
C
ZMNH, Universität Hamburg, Zentrum für Molekulare Neurobiologie, Institut für Neurale Signalverarbeitung, Falkenried 94, 20251 Hamburg, Germany.
Martin-Eauclaire
Marie-France
MF
Pongs
Olaf
O
Bougis
Pierre E
PE
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001667:Binding, Competitive / Q000187:drug effects; D018999:Charybdotoxin / Q000378:metabolism; D003101:Collodion; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007457:Iodine Radioisotopes; D007700:Kinetics; D051664:Kv1.3 Potassium Channel / Q000235:genetics / Q000378:metabolism*; D046228:Microarray Analysis / Q000295:instrumentation / Q000379:methods; D015221:Potassium Channels / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012604:Scorpion Venoms / Q000378:metabolism; D019789:Tetraethylammonium / Q000378:metabolism / Q000494:pharmacology; D014118:Toxins, Biological / Q000378:metabolism*
D001426:Bacterial Proteins; D007457:Iodine Radioisotopes; D051664:Kv1.3 Potassium Channel; D015221:Potassium Channels; D011993:Recombinant Fusion Proteins; D012604:Scorpion Venoms; D014118:Toxins, Biological; C110392:hongotoxin 1; C072276:kaliotoxin; C107790:prokaryotic potassium channel; D018999:Charybdotoxin; D019789:Tetraethylammonium; D003101:Collodion