title: Increased 8-OHdG levels in the urine, serum, and substantia nigra of hemiparkinsonian rats.
abstract: 8-Hydroxy-2'-deoxyguanosine (8-OHdG), the predominant marker of oxidative DNA damage, may be a good biomarker for monitoring the progression of Parkinson's disease (PD). Unfortunately, there are no basic laboratory data examining 8-OHdG levels in animal models of PD. In this study, we demonstrate that rats lesioned with 6-hydroxydopamine (6-OHDA) in the medial forebrain bundle display significantly elevated 8-OHdG levels in urine, serum, and substantia nigra, but not cerebrospinal fluid and striatum, compared to sham controls. These increments in 8-OHdG levels were detected at 2 days, but not at 7 days after the lesion suggesting that oxidative stress is restricted to the acute phase of 6-OHDA neurotoxicity. The present results support 8-OHdG as a biomarker that may aid both in the diagnosis and in the documentation of progression in PD.
authors.affiliation: Movement Disorders Program, Department of Neurology, Medical College of Georgia, 1429 Harper Street, HF-1121, Augusta, GA 30912, USA.
authors.forename: Takao
authors.initials: T
authors.lastname: Yasuhara
authors.forename: Koichi
authors.initials: K
authors.lastname: Hara
authors.forename: Kapil D
authors.initials: KD
authors.lastname: Sethi
authors.forename: John C
authors.initials: JC
authors.lastname: Morgan
authors.forename: Cesario V
authors.initials: CV
authors.lastname: Borlongan
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000378:metabolism / Q000652:urine; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism / Q000652:urine; D018450:Disease Progression; D009498:Neurotoxins; D018384:Oxidative Stress; D016627:Oxidopamine; D020734:Parkinsonian Disorders / Q000097:blood* / Q000503:physiopathology / Q000652:urine*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013378:Substantia Nigra / Q000378:metabolism* / Q000503:physiopathology; D013997:Time Factors; D015854:Up-Regulation
chemical_list: D015415:Biomarkers; D009498:Neurotoxins; D000080242:8-Hydroxy-2'-Deoxyguanosine; D016627:Oxidopamine; D003849:Deoxyguanosine