Real-time imaging of mechanically injured femoral artery in mice reveals a biphasic pattern of leukocyte accumulation.
Wire injury of an artery has been recognized as a standard model of vascular inflammation and atherosclerosis; however, the mechanism of leukocyte recruitment has not been studied in this model. In this study, we documented the recruitment of leukocytes to the murine femoral artery after a wire injury. A transluminal mechanical injury was generated by insertion of a wire into the femoral artery of male C57BL/6J mice. The mice were anesthetized and ventilated after tracheotomy and protected from hypothermia by a warming lamp. Body temperature and blood pH did not significantly change during the experiment. The interaction between rhodamine 6G-labeled leukocytes and the injured femoral artery was monitored using an epifluorescent microscope, and the images were evaluated using a computer-assisted image analysis program. In the absence of injury, virtually no leukocyte adhesion was observed. In contrast, the number of adherent leukocytes increased 4 and 24 h after injury and declined 72 h after injury. The rolling flux of leukocytes increased 4 h after injury and remained high up to 7 days, but it was faster 72 h after injury. We identified another peak of leukocyte adhesion 7 days after injury. Injection of anti-P-selectin antibody significantly reduced leukocyte adhesion at the early and later phases. In conclusion, we have established a novel experimental system for direct observation of leukocyte recruitment to the injured femoral artery. Our system revealed a previously undetected, unique profile of leukocyte recruitment during vascular injury.
Osaka
Mizuko
M
Life Science and Bioethics Research Center, Tokyo Medical and Dental University, School of Medicine, Keio University, Tokyo, Japan.
Hagita
Sumihiko
S
Haraguchi
Mihoko
M
Kajimura
Mayumi
M
Suematsu
Makoto
M
Yoshida
Masayuki
M
D000818:Animals; D001167:Arteritis / Q000276:immunology* / Q000473:pathology*; D015415:Biomarkers / Q000097:blood; D002465:Cell Movement / Q000276:immunology; D003199:Computer Systems; D005263:Femoral Artery / Q000276:immunology* / Q000293:injuries* / Q000473:pathology; D015850:Interleukin-6 / Q000097:blood; D007958:Leukocyte Count; D007962:Leukocytes / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018715:Microscopy, Video; D019007:P-Selectin / Q000378:metabolism
D015415:Biomarkers; D015850:Interleukin-6; D019007:P-Selectin