title: Depletion of endothelial progenitor cells in the peripheral blood of patients with ulcerative colitis.
abstract: There is strong evidence to suggest that endothelial progenitor cells (EPCs) play a significant role in re-endothelialization and subsequent tissue repair. This study examined the role of EPCs in inflammatory bowel disease, a disease in which impairment of mucosal healing has been implicated. Peripheral blood mononuclear cells obtained from 50 patients with ulcerative colitis (UC), 29 patients with Crohn's disease (CD), 14 patients with infectious colitis, and 35 normal control subjects were cultured in EPC medium, harvested after 7 days, and characterized by immunocytochemistry and flow cytometry. Colony assay for hematopoietic progenitor cells was also performed. Patients with active UC had a significantly decreased number of circulating EPCs as compared with healthy controls (p=0.0013), patients with inactive UC (p=0.0099), patients with active CD (p=0.0235) and patients with infectious colitis (p=0.0002). On the other hand, patients with infectious colitis had a significantly increased number of circulating EPCs as compared with healthy controls (p=0.0406), patients with active UC (p=0.0002), and patients with active CD (p=0.0316). In patients with UC, the number of circulating EPCs was correlated with the serum hemoglobin levels (r=0.485, p=0.007) and inversely with the platelet count (r=-0.372, p=0.0382). The number of hematopoietic progenitor cell colonies was comparable among patients with UC, patients with CD, patients with infectious colitis, and healthy controls. Our observations indicate that the number of circulating EPCs in patients with UC is significantly reduced. Further studies are needed to define the mechanisms that underlie the reduction in the number of circulating EPCs and to better understand the pathophysiological consequences of this event in patients with UC.
authors.affiliation: Division of Gastroenterology, Department of Medicine, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka 830-0011, Japan.
authors.forename: Junya
authors.initials: J
authors.lastname: Masuda
authors.forename: Keiichi
authors.initials: K
authors.lastname: Mitsuyama
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamasaki
authors.forename: Hiroko
authors.initials: H
authors.lastname: Takedatsu
authors.forename: Takashi
authors.initials: T
authors.lastname: Okamura
authors.forename: Akira
authors.initials: A
authors.lastname: Andoh
authors.forename: Toyoaki
authors.initials: T
authors.lastname: Murohara
authors.forename: Takayuki
authors.initials: T
authors.lastname: Asahara
authors.forename: Michio
authors.initials: M
authors.lastname: Sata
mesh_terms: D000328:Adult; D002452:Cell Count; D003093:Colitis, Ulcerative / Q000473:pathology*; D003114:Colony-Forming Units Assay; D042783:Endothelial Cells / Q000166:cytology*; D005260:Female; D006262:Health; D006801:Humans; D008297:Male; D013234:Stem Cells / Q000166:cytology*