Recombinant single chain Fv antibodies specific for glycoprotein D of equid herpesvirus 1.
Single chain Fv (scFv) molecules generated by phage-display technology represent a new and efficient tool in the research and diagnostics of infectious diseases. The recombinant glycoprotein D of Equid herpesvirus 1 was successfully expressed in E. coli cells. The protein was produced predominantly in soluble fraction and was then purified on a nickel-agarose column. The scFv antibodies against the glycoprotein were selected and several clones of glycoprotein D-specific scFv-antibodies were identified; t of them was expressed as a soluble scFv molecule, purified by immobilized metal-affinity chromatography and used as reagent in an immunofluorescence test.
Molinková
D
D
Institute of Microbiology and Immunology, Faculty of Veterinary Medicine, Veterinary and Pharmaceutical University Brno, 612 42 Brno, Czechia. molinkovad@vfu.cz
Celer
V
V
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity; D002460:Cell Line; D004285:Dogs; D004926:Escherichia coli / Q000378:metabolism; D004861:Herpesvirus 1, Equid / Q000276:immunology*; D007135:Immunoglobulin Variable Region / Q000096:biosynthesis / Q000276:immunology*; D019151:Peptide Library; D011994:Recombinant Proteins / Q000096:biosynthesis; D014759:Viral Envelope Proteins / Q000276:immunology* / Q000378:metabolism
D000914:Antibodies, Viral; D007135:Immunoglobulin Variable Region; D019151:Peptide Library; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C488988:glycoprotein D, Equid herpesvirus 1