Evaluation of cancer chemopreventive agents using clones derived from a human prostate cancer cell line.
BACKGROUND
The successful use of clonal selection through fluctuation analysis of human cancer cells as a means for studying tumor progression has been previously reported.


METHODS
Three clones derived from a parental population of human prostate cancer (LNCaP) cells were selected based on proliferation, hormone sensitivity and anchorage-independent growth. The effects of five potential cancer preventive agents were evaluated using cell proliferation, anchorage-independent growth and apoptosis as end-points.


RESULTS
Clone 21 cells, which represent a presumptive normal phenotype, were generally more sensitive than Clone 17 and Clone 6 cells, which represent a more malignant phenotype, to fluasterone, 7beta-HF, L-selenomethionine and troglitazone in assays for proliferation and/or apoptosis.


CONCLUSIONS
The results confirm the efficacy of the above agents as cancer chemopreventive agents and support our contention that clonal selection of established human cancer cells provides a model to study the efficacy of chemopreventive agents.
Ware
Jeffrey H
JH
Department of Radiation Oncology, Division of Oncology Research, University of Pennsylvania School of Medicine, 3620 Hamilton Walk, Philadelphia, PA 19104-6072, USA. jhware@mail.med.upenn.edu
Zhou
Zhaozong
Z
Kopelovich
Levy
L
Kennedy
Ann R
AR
D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000517:prevention & control*
D016588:Anticarcinogenic Agents