ESIPT-exhibiting protein probes: a sensitive method for rice proteins detection during starch extraction.
The 2-(4'-isothiocyanate-2'-hydroxyphenyl)benzoxazole dye was successfully applied as label of rice proteins during the alkaline extraction of starch. Direct fluorescence measurements were used to observe the presence of proteins labelled in different steps of rice starch extraction. The results were compared to those obtained with the well-known biuret colorimetric test. Whereas the colorimetric test indicates the absence of protein after the third extraction step, the fluorescence emission of the conjugate could be observed in all extraction steps. The separation of different rice proteins could also be observed.
Cardoso
Mateus Borba
MB
Universidade Federal do Rio Grande do Sul-Instituto de Química, Laboratório de Instrumentação e Dinâmica Molecular, Av. Bento Gonçalves, 9500. CP 15003 CEP 91501-970, Porto Alegre-RS, Brazil.
Samios
Dimitrios
D
da Silveira
Nádya Pesce
NP
Rodembusch
Fabiano Severo
FS
Stefani
Valter
V
D001583:Benzoxazoles / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D017879:Isothiocyanates / Q000737:chemistry*; D015335:Molecular Probes; D015394:Molecular Structure; D012275:Oryza / Q000737:chemistry*; D010940:Plant Proteins / Q000032:analysis* / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D013213:Starch / Q000737:chemistry* / Q000302:isolation & purification
C520959:2-(4'-isothiocyanate-2'-hydroxyphenyl)benzoxazole; D001583:Benzoxazoles; D005456:Fluorescent Dyes; D017879:Isothiocyanates; D015335:Molecular Probes; D010940:Plant Proteins; D013213:Starch