Electrochemically generated acid and its containment to 100 micron reaction areas for the production of DNA microarrays.
An addressable electrode array was used for the production of acid at sufficient concentration to allow deprotection of the dimethoxytrityl (DMT) protecting group from an overlaying substrate bound to a porous reaction layer. Containment of the generated acid to an active electrode of 100 micron diameter was achieved by the presence of an organic base. This procedure was then used for the production of a DNA array, in which synthesis was directed by the electrochemical removal of the DMT group during synthesis. The product array was found to have a detection sensitivity to as low as 0.5 pM DNA in a complex background sample.
Maurer
Karl
K
CombiMatrix Corporation, Mukilteo, Washington, United States of America. kmaurer@combimatrix.com
Cooper
John
J
Caraballo
Marcelo
M
Crye
James
J
Suciu
Dominic
D
Ghindilis
Andrey
A
Leonetti
Joseph A
JA
Wang
Wei
W
Rossi
Francis M
FM
Stöver
Axel G
AG
Larson
Christopher
C
Gao
Hetian
H
Dill
Kilian
K
McShea
Andy
A
D000143:Acids; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D055664:Electrochemical Techniques / Q000379:methods*; D007202:Indicators and Reagents; D008832:Microchemistry; D008839:Microelectrodes; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*
D000143:Acids; D017931:DNA Primers; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides