Photo-irradiation of Aloe vera by UVA--formation of free radicals, singlet oxygen, superoxide, and induction of lipid peroxidation.
Aloe vera whole leaf extracts are incorporated into a wide variety of topically applied commercial products. Aloe vera whole leaf extracts may contain anthraquinones, which have been shown to generate reactive oxygen species in the presence of ultraviolet A (UVA) light. Exposure to UVA light alone can also generate reactive oxygen species and is associated with photo-damaged and photo-aged skin in humans. This paper examines the photochemical properties of two Aloe vera whole leaf extracts that differed in their anthraquinone content. In the presence of methyl linoleate, the UVA irradiation of Aloe vera leaf extracts induced lipid peroxidation. The amounts of lipid peroxides formed were higher in the Aloe vera leaf extract that contained lower amounts of anthraquinones. Superoxide dismutase and sodium azide inhibited and deuterium oxide enhanced the formation of lipid peroxides, suggesting that singlet oxygen and superoxide were involved in the mechanism. Spin trapping electron spin resonance (ESR) spectroscopy was used to investigate the generation of free radicals by the UVA photo-irradiated Aloe vera plant extracts. ESR measurements indicated that the UVA photo-irradiation of Aloe vera plant extracts produced carbon-centered free radicals. These results suggest that humans exposed to products that contain Aloe vera whole leaf extracts may have enhanced sensitivity to ultraviolet light.
Xia
Qingsu
Q
National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USA.
Yin
Jun Jie
JJ
Fu
Peter P
PP
Boudreau
Mary D
MD
D000504:Aloe / Q000378:metabolism* / Q000528:radiation effects*; D004578:Electron Spin Resonance Spectroscopy; D004642:Emodin / Q000031:analogs & derivatives / Q000737:chemistry / Q000528:radiation effects; D016166:Free Radical Scavengers / Q000737:chemistry; D005609:Free Radicals / Q000737:chemistry*; D008041:Linoleic Acids / Q000737:chemistry; D015227:Lipid Peroxidation / Q000528:radiation effects*; D010936:Plant Extracts / Q000032:analysis / Q000528:radiation effects; D017382:Reactive Oxygen Species / Q000737:chemistry*; D013481:Superoxides / Q000737:chemistry*; D014466:Ultraviolet Rays
D016166:Free Radical Scavengers; D005609:Free Radicals; D008041:Linoleic Acids; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D013481:Superoxides; C005575:methyl linoleate; D004642:Emodin; C006457:alloin