[Identification of hydroxylate metabolites of daidzein and its sulfate conjugates in rat urine by LC-ESI/MS(n)].
OBJECTIVE
To identify the hydroxylate metabolites and its sulfate conjugates of daidzein in rat urine.


METHODS
Urine samples from 0 - 24 h were collected after single ig dose of 500 mg x kg(-1) daidzein to each of six rats. The urine samples were purified by SPE column (SPE C18) and analyzed with liquid chromatographic-tandem electrospray ionization ion trap mass spectrometry (LC-ESI/MS(n)) for potential metabolites.


RESULTS
Several new hydroxylate metabolites and its sulfate conjugates were found and identified in rat urine.


CONCLUSIONS
LC-ESI/MS(n) is proved to be a simple, rapid, sensitive and specific technique for identification of the hydroxylate metabolites and its sulfate conjugates of daidzein in rat urine.
Han
Feng-Mei
FM
Hubei Provincial Key Lab of Biotechnology of Traditional Chinese Medicine, Hubei University, Wuhan 430062, China.
Ge
Bao-Ying
BY
Chen
Huai-Xia
HX
Chen
Yong
Y
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D006900:Hydroxylation; D007529:Isoflavones / Q000737:chemistry / Q000378:metabolism* / Q000652:urine*; D008297:Male; D015394:Molecular Structure; D048789:Phytoestrogens / Q000737:chemistry / Q000378:metabolism / Q000652:urine; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry; D013025:Glycine max / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013431:Sulfates / Q000378:metabolism; D053719:Tandem Mass Spectrometry / Q000379:methods*
D007529:Isoflavones; D048789:Phytoestrogens; D013431:Sulfates; C004742:daidzein