title: Role of tumor necrosis factor-alpha, interleukin-8, and dexamethasone in the focal adhesion kinase expression by human nucleus pulposus cells.
abstract: METHODS
  Human nucleus pulposus cells from intervertebral disc specimens were cultured to study the effects of tumor necrosis factor (TNF)-alpha and interleukin (IL)-8 on the focal adhesion kinase (FAK) expression by these cells. The effect of co-stimulation with dexamethasone on the FAK expression by nucleus pulposus cells was also studied.
  
  
  OBJECTIVE
  To evaluate the possible role of activated FAK expressed by the human nucleus pulposus cells and its correlation with inflammatory cytokines (TNF-alpha, IL-8) and dexamethasone.
  
  
  BACKGROUND
  There have been no reported studies showing the correlation between the activated FAK expression by human nucleus pulposus cells with inflammatory cytokines and dexamethasone.
  
  
  METHODS
  The FAK expression in cultured human nucleus pulposus cells was studied, and Western blot and immunofluorescence analysis were performed to assess its relation to TNF-alpha, IL-8, and dexamethasone.
  
  
  RESULTS
  Treatments of TNF-alpha and IL-8 up-regulated the activated FAK expression. Dexamethasone attenuated the cytokine-induced FAK expression. The effects of inflammatory cytokines on the FAK expression were found to be concentration dependent, with greater correlation shown by IL-8 than TNF-alpha.
  
  
  CONCLUSIONS
  TNF-alpha and IL-8 stimulation up-regulated the FAK expression of human nucleus pulposus cells, and the coadministration of dexamethasone attenuated it.
authors.affiliation: Neuroscience Genome Research Center, The Catholic University of Korea, Seoul, Korea.
authors.forename: Bo Keun
authors.initials: BK
authors.lastname: Jee
authors.forename: Sibin
authors.initials: S
authors.lastname: Surendran
authors.forename: Koung Min
authors.initials: KM
authors.lastname: Park
authors.forename: Woon Kyu
authors.initials: WK
authors.lastname: Lee
authors.forename: Chang Whan
authors.initials: CW
authors.lastname: Han
authors.forename: Young Yul
authors.initials: YY
authors.lastname: Kim
authors.forename: Gopinathan
authors.initials: G
authors.lastname: Patinharayil
authors.forename: Young Hoon
authors.initials: YH
authors.lastname: Kim
authors.forename: Kweon Haeng
authors.initials: KH
authors.lastname: Lee
mesh_terms: D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000096:biosynthesis* / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D016209:Interleukin-8 / Q000494:pharmacology / Q000502:physiology*; D007403:Intervertebral Disc / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology / Q000502:physiology*
chemical_list: D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha; D003907:Dexamethasone; D051416:Focal Adhesion Protein-Tyrosine Kinases