Microsatellite marker analysis of peach-potato aphids (Myzus persicae, Homoptera: Aphididae) from Scottish suction traps.
The peach-potato aphid Myzus persicae (Sulzer) is an important vector of plant viruses. A network of suction traps collects aerial samples of this aphid in order to monitor and help predict its spatial distribution and likely impact on virus transmission in crops. A suction trap catch is thought to be a good representation of the total aphid pool. Sensitive molecular markers have been developed that determine the genetic composition of the M. persicae population. In Scotland, UK, these were applied to field collections revealing a limited number of clones. Molecular markers are less successful when applied to specimens that have been preserved in an ethanol-based trap fluid designed to preserve morphology. An assessment of different DNA extraction and PCR techniques is presented and the most efficient are used to analyse M. persicae specimens caught in the Dundee suction trap in 2001, a year when exceptionally high numbers were caught. The results reveal that the majority of the M. persicae caught belonged to two highly insecticide resistant clones. In addition, it was possible to compare the relative frequencies of genotypes caught in the trap with those collected at insecticide treated and untreated field sites in the vicinity. These results indicate that, in addition to suction trap data, the ability to sample field sites provides valuable early warning data which have implications for pest control and virus management strategies.
Malloch
G
G
Scottish Crop Research Institute, Invergowrie, Dundee, UK.
Highet
F
F
Kasprowicz
L
L
Pickup
J
J
Neilson
R
R
Fenton
B
B
D000818:Animals; D001042:Aphids / Q000235:genetics*; D004247:DNA / Q000302:isolation & purification; D005828:Genetics, Population; D005838:Genotype; D007300:Insect Control / Q000379:methods; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction / Q000379:methods; D012606:Scotland; D019914:Taq Polymerase; D013997:Time Factors
D004247:DNA; D019914:Taq Polymerase