mRNA expression and RNA editing (2451 C-to-U) of IL-12 receptor beta2 in adult atopic patients.
Interleukin (IL)-12 activates T helper (Th) 1 cells to produce interferon (IFN)-gamma which inhibits atopic inflammation. IL-12 acts through interaction with its receptor, especially beta(2) subunit. In several studies, the low production of IFN-gamma in peripheral mononuclear cells of atopic patients on response to IL-12 stimulation has been reported. Therefore we investigated the IL-12 receptor beta(2) (IL-12R beta(2)) mRNA expression and RNA editing, nucleotide 2451 C-to-U conversion, to find the cause of low responsiveness to IL-12 in atopy. Quantitative real time PCR for mRNA expression and sequence analysis for RNA editing were performed in 80 atopic patients and 54 healthy controls. The expression of IL-12R beta(2) mRNA was significantly lower in atopic patients than healthy controls (p<0.05). In sequence analysis, RNA editing on nucleotide 2451 was not found from either atopic patients or healthy controls. In additional evaluation, there was no relationship between expression of IL-12R beta(2) mRNA and serum total IgE or blood eosinophil count. Reduced IL-12R beta(2) mRNA expression in atopic patients indicate the reduced capacity to respond to IL-12 which induce IFN-gamma production and this may contribute to Th2-skewed immune response in atopy.
Kim
Eun Jin
EJ
Daegu Kyoungbook Blood Center, Korea Red Cross, Daegu, Korea.
Lee
Won Mok
WM
Ha
Jung Sook
JS
Ryoo
Nam Hee
NH
Jeon
Dong Suk
DS
Kim
Jae Ryong
JR
D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D005260:Female; D020022:Genetic Predisposition to Disease / Q000453:epidemiology / Q000235:genetics; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000453:epidemiology* / Q000235:genetics* / Q000378:metabolism; D007723:Korea / Q000453:epidemiology; D008297:Male; D017393:RNA Editing / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*; D053707:Receptors, Interleukin-12 / Q000235:genetics* / Q000378:metabolism; D015203:Reproducibility of Results; D018570:Risk Assessment / Q000379:methods*; D012307:Risk Factors; D012680:Sensitivity and Specificity
D015415:Biomarkers; C508439:IL12RB2 protein, human; D012333:RNA, Messenger; D053707:Receptors, Interleukin-12