Isolation of Lawsonia intracellularis specific single-chain Fv antibody fragments from phage display library.
Single-chain antibodies (scFv) exhibiting specific binding to Lawsonia intracellularis were isolated from a phagemid library expressing scFvs molecules on the surface of filamentous bacteriophages. For scFv selection whole bacterial cells were used and individual clones were tested in ELISA test. The total of seven unique clones with different fingerprint profiles was isolated. All clones were able to bind specifically in immunofluorescence assay. This is the first report of species specific recombinant antibodies against L. intracellularis.
Dezorzová-Tomanová
K
K
Institute of Microbiology and Immunology, Faculty of Veterinary Medicine, Veterinary and Pharmaceutical University Brno, 612 42 Brno, Czech Republic.
Molinková
D
D
Pekarová
M
M
Celer
V
V
Smola
J
J
D000907:Antibodies, Bacterial / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*; D000918:Antibody Specificity; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D020639:Lawsonia Bacteria / Q000276:immunology*; D019151:Peptide Library
D000907:Antibodies, Bacterial; D007135:Immunoglobulin Variable Region; D019151:Peptide Library