title: Mutation analysis of five candidate genes in familial breast cancer.
abstract: Most of the known breast cancer susceptibility genes (BRCA1, BRCA2, CHEK2 and ATM) are involved in the damage response pathway. Other members of this pathway are therefore good candidates for additional breast cancer susceptibility genes. ATR, along with ATM, plays a central role in DNA damage recognition and Chk1 relays checkpoint signals from both ATR and ATM. PPP2R1B and PPP2R5B code for subunits of protein phosphatase 2A (PP2A), which regulates autophosphorylation of ATM. In addition, EIF2S6/Int-6, which was originally identified as a common integration site for the mouse mammary tumour virus in virally induced mouse mammary tumours, is a candidate breast cancer susceptibility gene because of its putative role in maintaining chromosome stability. To investigate the role of ATR, CHK1, PPP2R1B, PPP2R5B and EIF2S6/Int-6, we carried out mutation analysis of these genes in the index cases from non-BRCA1/BRCA2 breast cancer families. We also screened sporadic breast tumours for somatic mutations in PPP2R1B and PPP2R5B. Although we identified many novel variants, we found no evidence that highly penetrant germline mutations in these five genes contribute to familial breast cancer susceptibility.
authors.affiliation: Cancer and Cell Biology, Queensland Institute of Medical Research, c/o RBH Post Office, Herston, Brisbane, QLD , 4029 , Australia.
authors.forename: Anna
authors.initials: A
authors.lastname: Marsh
authors.forename: Sue
authors.initials: S
authors.lastname: Healey
authors.forename: Aaron
authors.initials: A
authors.lastname: Lewis
authors.forename: Amanda B
authors.initials: AB
authors.lastname: Spurdle
authors.forename: Mary Anne
authors.initials: MA
authors.lastname: Kedda
authors.forename: Kum Kum
authors.initials: KK
authors.lastname: Khanna
authors.forename: Graham J
authors.initials: GJ
authors.lastname: Mann
authors.forename: Gulietta M
authors.initials: GM
authors.lastname: Pupo
authors.forename: Sunil R
authors.initials: SR
authors.lastname: Lakhani
authors.forename: Georgia
authors.initials: G
authors.lastname: Chenevix-Trench
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D017398:Alternative Splicing / Q000235:genetics; D019313:BRCA1 Protein / Q000235:genetics*; D024682:BRCA2 Protein / Q000235:genetics*; D001943:Breast Neoplasms / Q000235:genetics*; D000071877:Checkpoint Kinase 1; D015972:Gene Expression Regulation, Neoplastic; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D008875:Middle Aged; D009154:Mutation / Q000235:genetics; D011494:Protein Kinases / Q000235:genetics*; D054648:Protein Phosphatase 2 / Q000235:genetics*
chemical_list: D019313:BRCA1 Protein; D024682:BRCA2 Protein; D008565:Membrane Proteins; C432785:PPP2R1B protein, human; D011494:Protein Kinases; C000605867:CHEK1 protein, human; D000071877:Checkpoint Kinase 1; C000605869:Chek1 protein, mouse; C503493:PPP2R5B protein, human; D054648:Protein Phosphatase 2