title: Effects of photodynamic therapy using 5-aminolevulinic acid on cultured human adenomyosis-derived cells.
abstract: OBJECTIVE
  To evaluate the potential of photodynamic therapy with aminolevulinic acid (ALA) on human adenomyosis-derived cells compared with endometrial stromal cells.
  
  
  METHODS
  In vitro study.
  
  
  METHODS
  Department of Obstetrics and Gynecology, Tohoku University Hospital.
  
  
  METHODS
  Women with adenomyosis attending the University hospital.
  
  
  METHODS
  Photodynamic treatment.
  
  
  METHODS
  Cell survival rates.
  
  
  RESULTS
  Treatment with both ALA and irradiation caused significantly decreased survival of cells derived from human adenomyosis compared with ALA or irradiation alone. The combination of irradiation and ALA led to 79.3%, 68.0%, and 59.5% cell survival at 1.6, 4, and 8 J/cm2, respectively, whereas ALA and irradiation alone caused 92.8% and 97% survival, respectively.
  
  
  CONCLUSIONS
  Photodynamic therapy using ALA caused extensive death of cells derived from human adenomyosis. Photodynamic treatment using ALA may be a new treatment for patients with adenomyosis uteri in the future.
authors.affiliation: Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Miyagi, Japan. suharuka@mail.tains.tohoku.ac.jp
authors.forename: Haruka
authors.initials: H
authors.lastname: Suzuki-Kakisaka
authors.forename: Takashi
authors.initials: T
authors.lastname: Murakami
authors.forename: Toru
authors.initials: T
authors.lastname: Hirano
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Terada
authors.forename: Nobuo
authors.initials: N
authors.lastname: Yaegashi
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Okamura
mesh_terms: D000328:Adult; D000622:Aminolevulinic Acid / Q000008:administration & dosage*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D004715:Endometriosis / Q000188:drug therapy* / Q000473:pathology*; D004717:Endometrium / Q000187:drug effects* / Q000473:pathology*; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D008875:Middle Aged; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000008:administration & dosage; D016896:Treatment Outcome
chemical_list: D017319:Photosensitizing Agents; D000622:Aminolevulinic Acid