title: Histidine kinases play important roles in the perception and signal transduction of hydrogen peroxide in the cyanobacterium, Synechocystis sp. PCC 6803.
abstract: Oxidative stress caused by reactive oxygen species and, in particular, to hydrogen peroxide (H(2)O(2)) has a major impact on all biological systems, including plants and microorganisms. We investigated the H(2)O(2)-inducible expression of genes in the cyanobacterium Synechocystis sp. PCC 6803 using genome-wide DNA microarrays. Our systematic screening of a library of mutant lines with defects in histidine kinases (Hiks) by RNA slot-blot hybridization and DNA-microarray analysis suggested that four Hiks, namely, Hik33, Hik34, Hik16 and Hik41, are involved in the perception and transduction of H(2)O(2) signals that regulate the gene expression of 26 of the 77 H(2)O(2)-inducible genes with induction factors higher than 4.0. Among the four Hiks, Hik33 was the main contributor and was responsible for 22 of the 26 H(2)O(2)-inducible genes under the control of the Hiks. By contrast to Hik33, PerR encoding putative peroxide-sensing protein is involved in the regulation of only nine H(2)O(2)-inducible genes.
authors.affiliation: National Institute for Basic Biology, Okazaki 444-8585, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Kanesaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Kalyanee
authors.initials: K
authors.lastname: Paithoonrangsarid
authors.forename: Maria
authors.initials: M
authors.lastname: Shoumskaya
authors.forename: Iwane
authors.initials: I
authors.lastname: Suzuki
authors.forename: Hidenori
authors.initials: H
authors.lastname: Hayashi
authors.forename: Norio
authors.initials: N
authors.lastname: Murata
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D015152:Blotting, Northern; D000458:Cyanobacteria / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D000071677:Histidine Kinase; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008957:Models, Genetic; D020411:Oligonucleotide Array Sequence Analysis; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D007527:Isoenzymes; D006861:Hydrogen Peroxide; D011494:Protein Kinases; D000071677:Histidine Kinase