title: Protective effect of portal vein arterialization in acute liver failure induced by hepatectomy in normal and fatty liver rat.
abstract: OBJECTIVE
  We sought to determine whether an additional supply of oxygenated blood achieved by partial portal vein arterialization (PPVA) was protective on normal or fatty liver (FL) in rats with acute liver failure (ALF) induced by hepatectomy.
  
  
  METHODS
  Sprague-Dawley rats with normal or FL were segregated either to receive or not to undergo PPVA after hepatectomy. FL was induced by feeding a choline-deficient diet (5 days). PPVA was performed by anactamasing the left renal artery to the splenic vein with a stent following a left nephrectomy and splenectomy; the control rats underwent left nephrectomy and splenectomy only. Liver injury was evaluated by the serum alanine aminotransferase (ALT) level. The animals were sacrificed at 24 hours, 48 hours, and 7 days to collect blood and liver tissue samples for biochemical analysis. The 7-day survival was assessed in separate experimental groups.
  
  
  RESULTS
  PPVA significantly increased Po2 and oxygen saturation in the portal blood compared to non PPVA rats. PPVA significantly improved the 7-day survival compared with controls in both groups: hepatectomy of normal liver (90% vs 30%) and hepatectomy of FL (75% vs 25%). Serum ALT levels were slightly lower in the PPVA groups compared with the non-PPVA groups without a significant difference. Prothrombin activity decreased soon after hepatectomy in the normal and the FL liver groups but recovered rapidly thereafter without differences between the PPVA and non-PPVA treated animals.
  
  
  CONCLUSIONS
  An additional supply of arterial oxygenated blood through a PPVA promotes rapid resolution of ALF after partial hepatectomy in rats with normal or fatty livers, significantly improving 7-day survivals compared to hepatectomy controls.
authors.affiliation: Department of Surgery and Transplantation, University of Bologna, Bologna, Italy. nardo@orsola-malpighi.med.unibo.it
authors.forename: B
authors.initials: B
authors.lastname: Nardo
authors.forename: L
authors.initials: L
authors.lastname: Puviani
authors.forename: D
authors.initials: D
authors.lastname: Prezzi
authors.forename: F
authors.initials: F
authors.lastname: Neri
authors.forename: M
authors.initials: M
authors.lastname: Tsivian
authors.forename: B
authors.initials: B
authors.lastname: Mattioli
authors.forename: M
authors.initials: M
authors.lastname: Pariali
authors.forename: A M
authors.initials: AM
authors.lastname: Pertosa
authors.forename: P
authors.initials: P
authors.lastname: Caraceni
authors.forename: M
authors.initials: M
authors.lastname: Bernardi
authors.forename: A D
authors.initials: AD
authors.lastname: Pinna
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D002796:Choline Deficiency; D004195:Disease Models, Animal; D005234:Fatty Liver / Q000601:surgery*; D006498:Hepatectomy; D006499:Hepatic Artery / Q000601:surgery*; D017114:Liver Failure, Acute / Q000517:prevention & control*; D010100:Oxygen / Q000097:blood; D010101:Oxygen Consumption; D011168:Portal System / Q000502:physiology; D011169:Portal Vein / Q000601:surgery*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D010100:Oxygen