[Effects of emodin on proliferation cycle and apoptotic gene of human lung adenocarcinoma cell line Anip 973].
OBJECTIVE
To study the suppressive role of emodin on the growth and its effect on the proliferation cycle and apoptotic gene of human lung adenocarcinoma cell line Anip 973.


METHODS
The survival rate and the inhibitory rate of Anip 973 cell in vitro were detected by MTT colorimetric assay and cell growth curve assay at different time points under different concentration of emodin; the cell proliferation cycle and the apoptotic rate were examined with flow cytometry analysis, and Caspase-3 protein expression was measured by immunoblotting assay.


RESULTS
Emodin inhibited the proliferation of Anip 973 cell at G0/G1 phase, decreased the cell ratio at S phase and activated the Caspase-3 protein. It suppressed the growth of tumor cells and raised the apoptotic rate in a concentration and time depending manner in a certain extent.


CONCLUSIONS
Emodin could suppress the proliferation of Anip 973 cell, and its mechanism of anticancer effect may be through activating Caspase-3, to induce apoptosis and block cell cycle.
Li
Jia-ning
JN
Department of Respiratory Disease, The Second Affiliated Hospital of Harbin Medical University, Harbin. ljn1209475@sina.com.cn
Lv
Fu-zhen
FZ
Xiao
Jin-ling
JL
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology; D000368:Aged; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004642:Emodin / Q000494:pharmacology*; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology; D008297:Male
D000972:Antineoplastic Agents, Phytogenic; D053148:Caspase 3; D004642:Emodin