[Experimental study on the effects of jujingwan on oligospermia].
OBJECTIVE
To investigate the effects of Jujingwan on the spermatozoal ultrastructure and apoptosis of germ cells in oligospermia patients.


METHODS
We treated 50 oligospermia patients with Jujingwan and observed the spermatozoal ultrastructure, the apoptosis of germ cells and the changes in the DNA ploidy proportion of spermatogenic cells by electron microscopy and FCM before the treatment and 3, 6, 9 and 12 months after it.


RESULTS
Jujingwan increased sperm acrosome base density 6 months after the treatment and remarkably improved the integrity of acrosome membrane 12 months after it, with no obvious pathological changes in the nuclei and tails. Three months after the treatment, cell debris and apoptotic cells decreased significantly as compared with pre-treatment (P < 0. 05) , and very significantly 12 months after the treatment (P <0. 01). The proportion of haploid spermatozoa increased very significantly (P <0.01) , and the lost primary spermatocytes decreased significantly (P <0. 05) compared with pre-treatment.


CONCLUSIONS
Jujingwan can increase the density of sperm acrosome base and improve the pathological changes of acrosome membrane in oligospermia patients; it can improve the activity of acrosome enzyme and the integrity of acrosome membrane, decrease the apoptosis rate of germ cells and sperm and increase the percentage of haploid spermatozoa; it can also reduce the percentage of apoptotic bodies and diploid sperm cells. It is indicated that Jujingwan can inhibit the apoptosis of germ cells and sperm and improve spermatogenesis in oligospermia patients.
He
Ying
Y
Department of Andrology, Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing , Jiangsu 210029, China. swkr@sohu.com
Cao
Yan
Y
Xu
Yu-jian
YJ
Huang
Jian
J
Sun
Zhi-xing
ZX
Zhou
Zuo-min
ZM
Sha
Jia-hao
JH
Liu
Cheng-yong
CY
Wang
Qing
Q
Chi
Lei
L
Yang
Xiao-yu
XY
D000177:Acrosome / Q000473:pathology; D000328:Adult; D017209:Apoptosis; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D006801:Humans; D007248:Infertility, Male / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D009845:Oligospermia / Q000188:drug therapy* / Q000473:pathology; D008517:Phytotherapy; D013076:Sperm Count; D013090:Spermatocytes / Q000166:cytology; D013094:Spermatozoa / Q000648:ultrastructure
D004365:Drugs, Chinese Herbal