title: Periaqueductal gray cholecystokinin infusions block morphine-induced disruption of maternal behavior.
abstract: Cholecystokinin (CCK) and opiates interaction is critical for maintaining maternal behavior during lactation. Morphine inhibits while CCK restores maternal behavior. Recently we have shown that periaqueductal gray (PAG) is a region critically involved in the opioidergic blockade of maternal behavior. A critical level of morphine-induced activation of the rostral lateral PAG is required to inhibit maternal behavior in lactating rats. Since central CCK injections reverted morphine-induced inhibition of maternal behavior, we tested whether this peptide would act similarly in the PAG. This hypothesis was confirmed in experiments showing that morphine's inhibitory effect on maternal responsiveness was blocked by 1.0 and 0.2 nmol CCK injections into the rostral PAG, but not in nearby regions of the mesencephalic reticular nucleus. To test for possible compensatory changes the CCK2 receptor due to morphine treatments the expression of CCK2 receptor mRNA was evaluated in the PAG. PAG CCK2 receptor cDNA amplification revealed no difference in morphine treated animals. These results broaden understanding of the role played by CCK in the PAG. This CCK action might not depend on changes in its receptor.
authors.affiliation: Departamento de Patologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, SP 05508-900, Brazil.
authors.forename: Claudia M
authors.initials: CM
authors.lastname: Miranda-Paiva
authors.forename: Newton S
authors.initials: NS
authors.lastname: Canteras
authors.forename: Marcia H
authors.initials: MH
authors.lastname: Sukikara
authors.forename: Antonia G
authors.initials: AG
authors.lastname: Nasello
authors.forename: Ivone I
authors.initials: II
authors.lastname: Mackowiak
authors.forename: Luciano F
authors.initials: LF
authors.lastname: Felicio
mesh_terms: D000818:Animals; D005260:Female; D008297:Male; D008425:Maternal Behavior / Q000187:drug effects* / Q000502:physiology; D008845:Microinjections; D009020:Morphine / Q000494:pharmacology; D010487:Periaqueductal Gray / Q000187:drug effects* / Q000502:physiology; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D043887:Receptor, Cholecystokinin B / Q000235:genetics; D012844:Sincalide / Q000008:administration & dosage*
chemical_list: D012333:RNA, Messenger; D043887:Receptor, Cholecystokinin B; D009020:Morphine; D012844:Sincalide