Single nucleotide polymorphism identification, linkage and radiation hybrid mapping of the porcine pituitary adenylate cyclase-activating polypeptide type I receptor gene to chromosome 18.
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide with diverse biological actions. Type I PACAP receptors (PACAPR) are specific for PACAP, whereas type II and III PACAPRs are less restricted. To localize and analyse the variation of this gene, a 559-bp long intronic fragment of the porcine PACAPR gene was amplified by polymerase chain reaction and sequenced in samples from five different pig breeds. One single nucleotide polymorphism was identified and its allele frequency was determined in all five breeds. Linkage analysis in a Berkshire x Yorkshire reference family placed the PACAPR gene on chromosome 18, between SW787 and S0062 (SW787- 8.1 cM -PACAPR- 3.0 cM -S0062). Radiation hybrid mapping confirmed that the PACAPR gene was linked to SW1682 on chromosome 18 (28.8 cR(3000); LOD = 10.4).
Kollers
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S
Department of Pathology, Genus Cambridge Research Laboratory, Genus plc, University of Cambridge, Cambridge, UK.
Mote
B
B
Rothschild
M F
MF
Plastow
G
G
Rocha
D
D
D000818:Animals; D002874:Chromosome Mapping; D033481:Chromosomes, Mammalian; D005787:Gene Frequency; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D021101:Radiation Hybrid Mapping; D051237:Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I / Q000235:genetics*; D034421:Sus scrofa / Q000235:genetics*
D051237:Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I