title: [Study of Papaver somniferum cultivars identification by TD-RAPD technique].
abstract: OBJECTIVE
  The feasibility of Papaver somniferum L. cultivars identification was explored by TD-RAPD technique.
  
  
  METHODS
  Genomic DNA was extracted by improved CTAB method. One sample of species from Papaver somniferum L in xishuangbanna area. was studied by using TD-RAPD method.
  
  
  RESULTS
  We established an optimal method of extracting genomic DNA. Six primers were picked out from 10 primers.
  
  
  CONCLUSIONS
  TD-RAPD could be applied to researches of molecular marker of Papaver somniferum L. TD-RAPD technique provide a method to constitute DNA database of Papaver somniferum L. and conclude the source of opium poppy.
authors.affiliation: Yunnan Criminal Science and Technology Institute, Kunming 650021, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Lu
authors.forename: Jun-Yan
authors.initials: JY
authors.lastname: Hong
authors.forename: Rui
authors.initials: R
authors.lastname: He
authors.forename: Li-Sha
authors.initials: LS
authors.lastname: Li
mesh_terms: D017931:DNA Primers; D018744:DNA, Plant / Q000032:analysis* / Q000302:isolation & purification; D005240:Feasibility Studies; D006801:Humans; D010207:Papaver / Q000235:genetics*; D018515:Plant Leaves / Q000235:genetics; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D018744:DNA, Plant