Mesenchymal stem cell-mediated functional tooth regeneration in swine.
Mesenchymal stem cell-mediated tissue regeneration is a promising approach for regenerative medicine for a wide range of applications. Here we report a new population of stem cells isolated from the root apical papilla of human teeth (SCAP, stem cells from apical papilla). Using a minipig model, we transplanted both human SCAP and periodontal ligament stem cells (PDLSCs) to generate a root/periodontal complex capable of supporting a porcelain crown, resulting in normal tooth function. This work integrates a stem cell-mediated tissue regeneration strategy, engineered materials for structure, and current dental crown technologies. This hybridized tissue engineering approach led to recovery of tooth strength and appearance.
Sonoyama
Wataru
W
Center for Craniofacial Molecular Biology, University of Southern California School of Dentistry, Los Angeles, California, United States of America; Department of Oral and Maxillofacial Rehabilitation, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
Liu
Yi
Y
Fang
Dianji
D
Yamaza
Takayoshi
T
Seo
Byoung-Moo
BM
Zhang
Chunmei
C
Liu
He
H
Gronthos
Stan
S
Wang
Cun-Yu
CY
Wang
Songlin
S
Shi
Songtao
S
D000818:Animals; D003771:Dental Papilla / Q000166:cytology / Q000502:physiology; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D051379:Mice; D010513:Periodontal Ligament / Q000166:cytology / Q000502:physiology; D012038:Regeneration / Q000502:physiology*; D013552:Swine; D013556:Swine, Miniature; D014070:Tooth / Q000166:cytology / Q000502:physiology*; D014092:Tooth Root / Q000166:cytology / Q000502:physiology; D014183:Transplantation, Heterologous