Room-temperature, phosphorimetric determination of the beta-blocking agent pindolol in pharmaceutical tablets, urine and blood serum.
It is already recognised that heavy-atom-induced, room-temperature phosphorescence can be used to determine pindolol in pharmaceutical samples and biological fluids. We describe here a new, simple, rapid and selective development of this technique. The phosphorescence signals derive from the interaction of pindolol with a relatively high concentration of heavy-atom salts in the presence of sodium sulphite as oxygen scavenger. Phosphorescence was registered in the presence of 1.2 M potassium iodide, 15 mM sodium sulphite and 30% v/v methanol at 450 nm, exciting at 285 nm. The detection limit was 21.1 ng mL(-1). The method has been successfully applied to the determination of pindolol in commercial pharmaceutical tablets, urine and blood serum.
Salinas-Castillo
A
A
Department of Analytical Chemistry, Faculty of Sciences, University of Granada, c/ Fuentenueva s/n, 18071 Granada, Spain.
Cañabate-Díaz
B
B
Segura-Carretero
A
A
Fernández-Gutiérrez
A
A
D000319:Adrenergic beta-Antagonists / Q000032:analysis; D001774:Blood Chemical Analysis / Q000379:methods*; D008163:Luminescent Measurements / Q000379:methods*; D010869:Pindolol / Q000032:analysis* / Q000097:blood / Q000652:urine; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013607:Tablets / Q000737:chemistry*; D013696:Temperature; D016482:Urinalysis / Q000379:methods*
D000319:Adrenergic beta-Antagonists; D013607:Tablets; D010869:Pindolol