The KlSTE2 and KlSTE3 genes encode MATalpha- and MATa-specific G-protein-coupled receptors, respectively, which are required for mating of Kluyveromyces lactis haploid cells.
Mating in yeast is initiated by binding of pheromone to G-protein-coupled receptors expressed in haploid cells. We analysed the role of KlSte2p and KlSte3p receptors in the Kluyveromyces lactis mating pathway. By sequence analysis, KlSte2p and KlSte3p are the homologues of the Saccharomyces cerevisiae alpha-pheromone and a-pheromone receptors, respectively. However, by expression experiments, we determined that KlSTE2 gene is expressed in the cells typified as MATalpha and therefore is the receptor for the K. lactis a-pheromone and KlSTE3 gene is expressed in the MATa cells and binds the alpha-pheromone. The KlSTE2 gene is silent in MATa cells, while it is highly expressed in MATalpha cells, and conversely the KlSTE3 gene is expressed in MATa cells and repressed in MATalpha cells. Disruption mutants of both genes were found to be sterile, and this defect is reversed by plasmidic copies of each gene. The cytoplasmic C-terminus of KlSte3p interacts strongly with the KlGpa1p (Galpha) subunit, which is involved in the transduction of the pheromone stimulus to induce mating. Remarkably, this same domain does not interact with a constitutive active allele of the Galpha subunit, indicating that the C-terminus is able to discriminate between the active (GTP-bound) and inactive (GDP-bound) forms of the Galpha subunit.
Torres-Quiroz
Francisco
F
Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Apartado Postal 70-242, 04510 México, D.F., México.
Kawasaki
Laura
L
Rodríguez-González
Miriam
M
Patrón-Soberano
Araceli
A
Coria
Roberto
R
D000595:Amino Acid Sequence; D015152:Blotting, Northern; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D049770:Genes, Mating Type, Fungal / Q000235:genetics / Q000502:physiology*; D006238:Haploidy; D007716:Kluyveromyces / Q000235:genetics / Q000502:physiology*; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010675:Pheromones / Q000235:genetics / Q000502:physiology; D016133:Polymerase Chain Reaction; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000502:physiology*; D043563:Receptors, Pheromone / Q000235:genetics / Q000502:physiology; D016415:Sequence Alignment
D004271:DNA, Fungal; D010675:Pheromones; D043562:Receptors, G-Protein-Coupled; D043563:Receptors, Pheromone