title: Cell interaction with three-dimensional sharp-tip nanotopography.
abstract: Cells in their native microenvironment interact with three-dimensional (3D) nanofeatures. Despite many reports on the effects of substrate nanotopography on cells, the independent effect of 3D parameters has not been investigated. Recent advances in nanofabrication for precise control of nanostructure pattern, periodicity, shape, and height enabled this systematic study of cell interactions with 3D nanotopographies. Two distinct nanopatterns (posts and grates) with varying three-dimensionalities (50-600 nm in nanostructure height) were created, while maintaining the pattern periodicity (230 nm in pitch) and tip shape (needle- or blade-like sharp tips). Human foreskin fibroblasts exhibited significantly smaller cell size and lower proliferation on needle-like nanoposts, and enhanced elongation with alignment on blade-like nanogrates. These phenomena became more pronounced as the nanotopographical three-dimensionality (structural height) increased. The nanopost and nanograte architectures provided the distinct contact guidance for both filopodia extension and the formation of adhesion molecules complex, which was believed to lead to the unique cell behaviors observed.
authors.affiliation: Mechanical and Aerospace Engineering Department, University of California, Los Angeles, CA 90095, USA. chchoi@ucla.edu
authors.forename: Chang-Hwan
authors.initials: CH
authors.lastname: Choi
authors.forename: Sepideh H
authors.initials: SH
authors.lastname: Hagvall
authors.forename: Benjamin M
authors.initials: BM
authors.lastname: Wu
authors.forename: James C Y
authors.initials: JC
authors.lastname: Dunn
authors.forename: Ramin E
authors.initials: RE
authors.lastname: Beygui
authors.forename: Chang-Jin
authors.initials: CJ
authors.lastname: CJ Kim
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D048429:Cell Size; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D008422:Materials Testing; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods; D012825:Silicon / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D012825:Silicon