Generating unigene collections of expressed sequence tag sequences for use in mass spectrometry identification.
Expressed sequence tag sequences remain the largest resource of DNA sequence for most organisms despite recent advances in genome sequencing. These sequences are short, fragmented versions of the expressed genes. By DNA sequence assembly, the fragments can be assembled into contiguous DNA sequences that are better suited for protein identification by mass spectrometry.
Emmersen
Jeppe
J
Department of Life Sciences, Aalborg University, Denmark.
D016000:Cluster Analysis; D030561:Databases, Nucleic Acid; D030562:Databases, Protein; D020224:Expressed Sequence Tags; D013058:Mass Spectrometry / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*; D020539:Sequence Analysis, Protein; D012984:Software