YjjG, a dUMP phosphatase, is critical for thymine utilization by Escherichia coli K-12.
Exogenous thymine must be converted to thymidine to enable a thyA (thymidylate synthase) mutant to grow. The deoxyribose in the thymidine comes from dUMP, which must first be dephosphorylated. The nucleotidase YjjG is critical for this step. A yjjG thyA mutant cannot use thymine for growth on a glucose minimal medium.
Weiss
Bernard
B
Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA 30322, USA. bweiss2@emory.edu
D003856:Deoxyuracil Nucleotides / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000502:physiology*; D009699:N-Glycosyl Hydrolases / Q000502:physiology*; D010766:Phosphorylation; D013940:Thymidylate Synthase / Q000502:physiology; D013941:Thymine / Q000378:metabolism*
D003856:Deoxyuracil Nucleotides; D029968:Escherichia coli Proteins; C007267:2'-deoxyuridylic acid; D013940:Thymidylate Synthase; C497359:YjjG protein, E coli; D009699:N-Glycosyl Hydrolases; D013941:Thymine