Quantitation with virtual expert mass spectrometrist.
Quantitative analysis of proteins and peptides by mass spectrometry has been greatly advanced by the development of proteomic technologies within recent years. Particularly, labeling of peptides and proteins with stable isotopes such as 2H, 15N, and 13C facilitated the unbiased comparison of protein amounts in distinct samples in a single mass spectrometric experiment. These methods can be applied to detect quantitative changes in protein amounts and posttranslational modifications such as phosphorylation. Quantitation of mass spectra requires accurate and efficient bioinformatics tools, which can match corresponding peptides, determine peak intensities, and calculate relative amounts. In this chapter, we describe the use of virtual expert mass spectrometrist for the quantitation of mass spectra from samples with peptides and proteins encoded with stable isotopes.
Gruhler
Albrecht
A
Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense.
Matthiesen
Rune
R
D019295:Computational Biology / Q000379:methods*; D007553:Isotope Labeling; D013058:Mass Spectrometry / Q000379:methods*; D010455:Peptides / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D012984:Software
D010455:Peptides; D011506:Proteins