Genome sequencing analysis of Brazilian chicken anemia virus isolates that lack MSB-1 cell culture tropism.
Specific amino acid (aa) substitutions in VP1, VP2 and VP3 genes were reported as a distinctive feature of the American CIA-1 strain, characterized as having a variable rate of growth and tropism for different MSB-1 cell sublines [Renshaw RW, Soiné C, Weinkle T, O'Connell PH, Ohashi K, Watson S, et al. A hypervariable region in VP1 of chicken anemia virus mediates rate of spread and cell tropism in tissue culture. J Virol 1996;70(12):8872-8]. DNA sequencing of 878 nucleotides from twelve Brazilian CAV, eight of which tested for in vitro isolation in three different sources of MDCC-MSB1 cell line and identified as lacking capacity to propagate in any of these cells, were compared to sequence data available for CAV strains propagated or not in cell culture. Alignment of the deduced aa resulted in a lack of singled out amino acid substitutions in the partial genomic sequences of Brazilian isolates that would entirely contrast them to viruses propagated in MSB-1 cells, indicating that the combined VP1, VP2 and VP3 substitutions observed may not entirely account as sole determinants of CAV isolation and propagation in MDCC-MSB-1 cells.
Nogueira
Eliana Ottati
EO
Department of Pathology, College of Veterinary Medicine, University of São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, 05508-900 São Paulo, SP, Brazil.
J Piantino Ferreira
Antonio
A
Martins Soares
Rodrigo
R
Luiz Durigon
Edison
E
Lazzarin
Simaia
S
Brentano
Liana
L
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001938:Brazil; D002460:Cell Line; D017727:Chicken anemia virus / Q000145:classification / Q000235:genetics* / Q000502:physiology; D002645:Chickens; D018173:Circoviridae Infections / Q000662:veterinary* / Q000821:virology; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D011201:Poultry Diseases / Q000821:virology*; D016415:Sequence Alignment / Q000662:veterinary; D018523:Tropism / Q000235:genetics
D004279:DNA, Viral