Reduced diversity of the human erythrocyte membrane sialic acids in polycythemia vera. Absence of N-glycolylneuraminic acid and characterisation of N-acetylneuraminic acid 1,7 lactone.
Sialic acids from the erythrocyte (RBC) membrane of a patient suffering from polycythemia vera, a malignant orphan disorder of hematopoietic cells, was studied using GC/MS. We found that the sialic acid diversity of these membranes was drastically reduced since only four entities were identified: Neu5Ac (91.5%) and its 1,7 lactone Neu5Ac1,7L (7.5%) which is absent in normal RBC, Neu4,5Ac(2) (0.50%) and Neu4,5Ac(2) 9Lt (0.50%); in normal RBC, Neu5,7Ac(2), Neu5,9Ac(2), Neu5Ac9Lt, Neu5Ac8S and Neu, as well as traces of Kdn, were also present. Neu5Gc and its O-alkylated or O-acetylated derivatives, which are considered by various authors as cancer markers, were not detected.
Bratosin
Daniela
D
Institutul National de Cercetare-Dezvoltare pentru Stiinte Biologice, Bucuresti, Romania. bratosind@yahoo.com
Palii
Carmen
C
Moicean
Andreea Delia
AD
Zanetta
Jean-Pierre
JP
Montreuil
Jean
J
D004910:Erythrocyte Membrane / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007783:Lactones / Q000032:analysis / Q000737:chemistry; D015394:Molecular Structure; D009438:Neuraminic Acids / Q000032:analysis / Q000737:chemistry; D011087:Polycythemia Vera / Q000378:metabolism* / Q000473:pathology; D012794:Sialic Acids / Q000032:analysis* / Q000737:chemistry
D007783:Lactones; D009438:Neuraminic Acids; D012794:Sialic Acids; C032592:N-glycolylneuraminic acid