A new fermentation process allows large-scale production of tetra-N-acetyl-chitotetraosyl allosamizoline.
A new compound 2, possessing a tetra-N-acetyl-chitotetraosyl moiety as a constituent, was synthesized by bacterial fermentation, which used allosamizoline 1 as the initial acceptor. A 2-binding chitinase assay, indicated that the chitinase was inactivated by 2 with IC50 = 0.03 microg/mL.
Huang
Gang-Liang
GL
School of Life Science, Shandong University, Jinan City 250100, China. hgl226@126.com
Mei
Xin-Ya
XY
Zhang
Hou-Cheng
HC
Wang
Peng-George
PG
D002236:Carbohydrate Conformation; D002688:Chitinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D005285:Fermentation; D005944:Glucosamine / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology; D011485:Protein Binding
D004791:Enzyme Inhibitors; C512151:tetra-N-acetyl-chitotetraosyl allosamizoline; D002688:Chitinases; D005944:Glucosamine