Gateway-based destination vectors for functional analyses of bacterial ORFeomes: application to the Min system in Brucella abortus.
Twenty Gateway-compatible destination vectors were constructed. The vectors comprise fluorescent and epitope fusion tags, various drug markers, and replication origins that should make them useful for exploring existing microbial ORFeomes. In an attempt to validate several of these vectors, we observed polar and oscillating localization of MinD in Brucella abortus.
Hallez
Régis
R
Unité de Recherche en Biologie Moléculaire, University of Namur, 61 rue de Bruxelles, B-5000 Namur, Belgium.
Letesson
Jean-Jacques
JJ
Vandenhaute
Jean
J
De Bolle
Xavier
X
D002003:Brucella abortus / Q000235:genetics* / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D020543:Proteome / Q000378:metabolism*; D014169:Transformation, Bacterial
D020543:Proteome