[ADLib system for rapid and flexible design of monoclonal antibodies].
Monoclonal antibodies (MAbs) have been utilized as research tools, as diagnostic reagents, and for antibody medicine. The preparation of MAbs involves laborious processes and normally takes months. Here we describe an ex vivo B cell-based antibody display system called the ADLib (Autonomously Diversifying Library) system, which enables us to select chicken B cell clones producing antibody against antigens of interest in a couple of weeks. The ADLib system is applicable to self- or highly conserved antigens, polysaccharide chains, peptides, and haptens.
Ohta
Kunihiro
K
Genetic System Regulation Laboratory, RIKEN Discovery Research Institute, Wako City, Saitama, Japan. kohta@riken.jp
Seo
Hidetaka
H
D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D002645:Chickens / Q000235:genetics; D005785:Gene Conversion; D007118:Immunoassay; D019151:Peptide Library; D015202:Protein Engineering / Q000379:methods*
D000911:Antibodies, Monoclonal; D019151:Peptide Library