Green tea protects cytoskeleton from oxidative injury in cardiomyocytes.
Cardiac ischemia/reperfusion injury results in oxidative stress and poor physiological recovery. Episodes of hypoxia/reoxygenation (H/R) cause some subtle functional and structural alterations in sarcolemma, mithocondria, sarcoplasmic reticulum, nucleus, as well as cytoskeleton. In this report, by using cultured rat cardiomyocytes and laser confocal microscopy we have verified the possibility to counteract cytoskeleton alterations induced by H/R with the supplementation of an antioxidant agent, a green tea extract (GTE), and compared its effects to those of alpha-tocopherol. Moreover the effects of GTE on cell viability and cytosolic antioxidant activity have been evaluated. H/R induced myocardial damage occurs as histological alterations such as degeneration and disorganization of the cytoskeleton and loss of structural integrity of the nucleus. GTE supplementation increases cytosolic antioxidant activity and shows protective effects on cardiomyocyte cytoarchitecture and viability.
Pagnotta
Eleonora
E
Department of Biochemistry "G. Moruzzi", Alma Mater Studiorum, University of Bologna, Via Irnerio 48, 40126 Bologna, Italy. eleonora.pagnotta3@unibo.it
Calonghi
Natalia
N
Hrelia
Silvana
S
Masotti
Lanfranco
L
Biagi
Pierluigi
P
Angeloni
Cristina
C
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D028241:Camellia sinensis / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000187:drug effects*; D018613:Microscopy, Confocal; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000648:ultrastructure*; D018384:Oxidative Stress; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013662:Tea / Q000737:chemistry*
D000975:Antioxidants; D010936:Plant Extracts; D013662:Tea