title: Optical imaging of bacterial infection in living mice using a fluorescent near-infrared molecular probe.
abstract: An optical imaging probe was synthesized by attaching a near-infrared carbocyanine fluorophore to an affinity group containing two zinc(II) dipicolylamine (Zn-DPA) units. The probe has a strong and selective affinity for the surfaces of bacteria, and it was used to image infections of Gram-positive S. aureus and Gram-negative E. coli bacteria in living nude mice. After intravenous injection, the probe selectively accumulates at the sites of localized bacterial infections in the thigh muscles of the mice.
authors.affiliation: Department of Chemistry and Biochemistry, 251 Nieuwland Science Hall, University of Notre Dame, Notre Dame, IN 46556, USA.
authors.forename: W Matthew
authors.initials: WM
authors.lastname: Leevy
authors.forename: Seth T
authors.initials: ST
authors.lastname: Gammon
authors.forename: Hua
authors.initials: H
authors.lastname: Jiang
authors.forename: James R
authors.initials: JR
authors.lastname: Johnson
authors.forename: Dustin J
authors.initials: DJ
authors.lastname: Maxwell
authors.forename: Erin N
authors.initials: EN
authors.lastname: Jackson
authors.forename: Manuel
authors.initials: M
authors.lastname: Marquez
authors.forename: David
authors.initials: D
authors.lastname: Piwnica-Worms
authors.forename: Bradley D
authors.initials: BD
authors.lastname: Smith
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D051379:Mice; D015335:Molecular Probes / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D010847:Picolines / Q000138:chemical synthesis / Q000737:chemistry*; D012680:Sensitivity and Specificity; D019265:Spectroscopy, Near-Infrared / Q000379:methods; D013203:Staphylococcal Infections / Q000175:diagnosis*; D013211:Staphylococcus aureus / Q000737:chemistry; D041622:Tomography, Optical / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D015335:Molecular Probes; D009942:Organometallic Compounds; D010847:Picolines; C509592:zinc(II) dipicolylamine