title: Comparison of Cepheid's analyte-specific reagents with BD directigen for detection of respiratory syncytial virus.
abstract: For detection of respiratory syncytial virus (RSV), the BD Directigen RSV rapid antigen assay was compared to Cepheid's real-time reverse transcriptase PCR RSV analyte-specific reagents. The Directigen RSV assay resulted in a 23% false-negative rate, using PCR and chart review as the gold standard, indicating that rapid RSV PCR results would be advantageous.
authors.affiliation: University of North Carolina Hospitals, Clinical Microbiology-Immunology Laboratory, Chapel Hill, NC, USA.
authors.forename: Jennifer S
authors.initials: JS
authors.lastname: Goodrich
authors.forename: Melissa B
authors.initials: MB
authors.lastname: Miller
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000956:Antigens, Viral / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D005188:False Negative Reactions; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D007231:Infant, Newborn; D008875:Middle Aged; D009305:Nasopharynx / Q000821:virology; D019590:Nucleocapsid Proteins / Q000235:genetics*; D011933:Reagent Kits, Diagnostic; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis / Q000821:virology*; D018113:Respiratory Syncytial Virus, Human / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D000956:Antigens, Viral; D019590:Nucleocapsid Proteins; D011933:Reagent Kits, Diagnostic