title: Netrin/DCC signaling controls contralateral dendrites of octavolateralis efferent neurons.
abstract: The guidance molecule Netrin and its receptor DCC (deleted in colorectal cancer) attract commissural axons toward the midline en route to their final destination. To test whether these molecules can also guide dendrites, we studied the contralateral dendrites of zebrafish octavolateralis efferent (OLe) neurons, which are unusual in that they navigate toward and cross the midline. We found that, at the time of dendrite outgrowth, OLe neurons express dcc, and the hindbrain midline expresses netrin1. Knocking down dcc or netrin1 function by injecting antisense morpholino oligonucleotides prevented OLe contralateral dendrites from crossing the midline, showing that dcc and netrin1 are necessary for dendrite guidance or formation. Furthermore, by transplanting cells from dcc morphants into wild-type embryos and vice versa, we demonstrated that dcc acts cell autonomously in OLe dendrites. This work is the first evidence that Netrin/DCC signaling acts in dendrites in a vertebrate system.
authors.affiliation: Department of Neurobiology and Anatomy, University of Utah, Salt Lake City, Utah 84132, USA.
authors.forename: Arminda
authors.initials: A
authors.lastname: Suli
authors.forename: Nathan
authors.initials: N
authors.lastname: Mortimer
authors.forename: Iain
authors.initials: I
authors.lastname: Shepherd
authors.forename: Chi-Bin
authors.initials: CB
authors.lastname: Chien
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D000075389:DCC Receptor; D003712:Dendrites / Q000235:genetics / Q000502:physiology*; D016332:Fetal Tissue Transplantation / Q000379:methods; D009414:Nerve Growth Factors / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D000075388:Netrin-1; D009476:Neurons, Efferent / Q000166:cytology / Q000502:physiology*; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D012249:Rhombencephalon / Q000166:cytology / Q000196:embryology / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*
chemical_list: D000075389:DCC Receptor; D009414:Nerve Growth Factors; D011956:Receptors, Cell Surface; D025521:Tumor Suppressor Proteins; D029961:Zebrafish Proteins; C506552:dcc protein, zebrafish; D000075388:Netrin-1