title: Interaction of rice dwarf virus outer capsid P8 protein with rice glycolate oxidase mediates relocalization of P8.
abstract: Yeast two-hybrid and coimmunoprecipitation assays indicated that P8, an outer capsid protein of Rice dwarf phytoreovirus (RDV), interacts with rice glycolate oxidase (GOX), a typical enzyme of peroxisomes. Confocal immunofluorescence microscopy revealed that P8 was colocalized with GOX in peroxisomes. Time course analysis demonstrated that the localization of P8 in Spodoptera frugiperda cells changed from diffuse to discrete, punctuate inclusions during expression from 24 to 48 h post inoculation. Coexpression of GOX with P8 may target P8 into peroxisomes, which serve as replication sites for a number of viruses. Therefore, we conclude that the interaction of P8 with the GOX of host cells leads to translocation of P8 into peroxisomes and we further propose that the interaction between P8 and GOX may play important roles in RDV targeting into the replication site of host cells. Our findings have broad significance in studying the mechanisms whereby viruses target appropriate replication sites and begin their replication.
authors.affiliation: Peking-Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology, The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhou
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
authors.forename: Wulan
authors.initials: W
authors.lastname: Deng
authors.forename: Yingying
authors.initials: Y
authors.lastname: Pu
authors.forename: Chunhong
authors.initials: C
authors.lastname: Wei
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D012275:Oryza / Q000201:enzymology* / Q000821:virology; D020675:Peroxisomes / Q000201:enzymology* / Q000821:virology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport / Q000502:physiology; D012087:Reoviridae / Q000235:genetics / Q000378:metabolism*; D018411:Spodoptera / Q000166:cytology / Q000378:metabolism; D014779:Virus Replication / Q000502:physiology*
chemical_list: D036022:Capsid Proteins; D010940:Plant Proteins; D000429:Alcohol Oxidoreductases; C020821:glycollate oxidase