RBMX is a novel hepatic transcriptional regulator of SREBP-1c gene response to high-fructose diet.
In rodents a high-fructose diet induces metabolic derangements similar to those in metabolic syndrome. Previously we suggested that in mouse liver an unidentified nuclear protein binding to the sterol regulatory element (SRE)-binding protein-1c (SREBP-1c) promoter region plays a key role for the response to high-fructose diet. Here, using MALDI-TOF MASS technique, we identified an X-chromosome-linked RNA binding motif protein (RBMX) as a new candidate molecule. In electrophoretic mobility shift assay, anti-RBMX antibody displaced the bands induced by fructose-feeding. Overexpression or suppression of RBMX on rat hepatoma cells regulated the SREBP-1c promoter activity. RBMX may control SREBP-1c expression in mouse liver in response to high-fructose diet.
Takemoto
Tadashi
T
Division of Molecular Genetics in Medicine, Department of Biochemistry and Molecular Biology, Shiga University of Medical Science, Shiga 520-2192, Japan.
Nishio
Yoshihiko
Y
Sekine
Osamu
O
Ikeuchi
Chikako
C
Nagai
Yoshio
Y
Maeno
Yasuhiro
Y
Maegawa
Hiroshi
H
Kimura
Hiroshi
H
Kashiwagi
Atsunori
A
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D045744:Cell Line, Tumor; D004032:Diet; D024202:Electrophoretic Mobility Shift Assay; D005632:Fructose / Q000008:administration & dosage*; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D011401:Promoter Regions, Genetic / Q000187:drug effects; D034622:RNA Interference; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics*
D000906:Antibodies; D034441:Heterogeneous-Nuclear Ribonucleoproteins; C518069:RBMX protein, mouse; C497388:Srebf1 protein, mouse; D051780:Sterol Regulatory Element Binding Protein 1; D005632:Fructose