title: Synthesis and characterization of poly (amino ester) for slow biodegradable gene delivery vector.
abstract: Many therapeutic carrier materials were exploited for human gene therapy from viral to polymeric vectors. This research describes the evaluation of two biodegradable ester-bonded polymers synthesized by double-monomer polycondensation for a non-viral cationic polymer-based gene delivery system. The backbone was constructed to include inner tertiary amines and outer primary amines. Self-assembly with DNA resulted in the production of regularly nano-sized spherical polyplexes with good transfection efficiency, especially in the presence of serum. The polymers showed a relatively slow degradability for an amine-containing ester polymer, as they maintained DNA/polymer complex for 7 days in physiological buffer conditions. Finally, the low toxicity and slow degradation concluded these polymers reliable for long-term therapeutic applications.
authors.affiliation: School of Chemistry and Molecular Engineering, Seoul National University, San 56-1, Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
authors.forename: Hyun Jin
authors.initials: HJ
authors.lastname: Kim
authors.forename: Min Sung
authors.initials: MS
authors.lastname: Kwon
authors.forename: Joon Sig
authors.initials: JS
authors.lastname: Choi
authors.forename: Bo Hye
authors.initials: BH
authors.lastname: Kim
authors.forename: Jae Keun
authors.initials: JK
authors.lastname: Yoon
authors.forename: Kwan
authors.initials: K
authors.lastname: Kim
authors.forename: Jong-Sang
authors.initials: JS
authors.lastname: Park
mesh_terms: D002021:Buffers; D004247:DNA / Q000378:metabolism; D004355:Drug Stability; D018014:Gene Transfer Techniques; D006801:Humans; D053758:Nanoparticles; D011091:Polyesters / Q000138:chemical synthesis* / Q000627:therapeutic use* / Q000633:toxicity; D044967:Serum; D014162:Transfection
chemical_list: D002021:Buffers; D011091:Polyesters; D004247:DNA