title: Tetraphenylphosphonium-selective electrode as a tool for evaluating mitochondrial permeability transition pore function in isolated rat hepatocytes.
abstract: The changes in mitochondrial membrane potential (Deltapsi(m)) were used as an indicator for evaluating the mitochondrial permeability transition pore (MPTP) function. We found that in situ mitochondria in digitonin-permeabilized hepatocytes were coupled and responded to the addition of substrates, inhibitors and uncouplers. Ca(2+)-induced Deltapsi(m) dissipation was caused by MPTP opening because this process was inhibited by cyclosporin A. MPTP opening was enhanced by the pro-oxidant tert-butyl hydroperoxide.
authors.affiliation: Institute of Pathophysiology, 1st Faculty of Medicine, Charles University, U Nemocnice 5, 120 00 Prague 2, Czech Republic. Anna.Labajova@lf1.cuni.cz
authors.forename: A
authors.initials: A
authors.lastname: Lábajová
authors.forename: J
authors.initials: J
authors.lastname: Kofránek
authors.forename: P
authors.initials: P
authors.lastname: Kriváková
authors.forename: Z
authors.initials: Z
authors.lastname: Cervinková
authors.forename: Z
authors.initials: Z
authors.lastname: Drahota
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology; D004566:Electrodes; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D053078:Membrane Potential, Mitochondrial; D033681:Mitochondrial Membrane Transport Proteins / Q000378:metabolism*; D000083162:Mitochondrial Permeability Transition Pore; D009861:Onium Compounds; D009943:Organophosphorus Compounds; D010101:Oxygen Consumption; D051381:Rats; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
chemical_list: D007202:Indicators and Reagents; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D009861:Onium Compounds; D009943:Organophosphorus Compounds; D020122:tert-Butylhydroperoxide; D002118:Calcium; C013289:tetraphenylphosphonium