title: Diabetes mellitus attenuates the repolarization reserve in mammalian heart.
abstract: OBJECTIVE
  In diabetes mellitus several cardiac electrophysiological parameters are known to be affected. In rodent experimental diabetes models changes in these parameters were reported, but no such data are available in other mammalian species including the dog. The present study was designed to analyse the effects of experimental type 1 diabetes on ventricular repolarization and its underlying transmembrane ionic currents and channel proteins in canine hearts.
  
  
  RESULTS
  Diabetes was induced by a single injection of alloxan, a subgroup of dogs received insulin substitution. After the development of diabetes (8 weeks) electrophysiological studies were performed using conventional microelectrodes, whole cell voltage clamp, and ECG. Expression of ion channel proteins was evaluated by Western blotting. The QTc interval and the ventricular action potential duration in diabetic dogs were moderately prolonged. This was accompanied by significant reduction in the density of the transient outward K+ current (I(to)) and the slow delayed rectifier K+ current (I(Ks)), to 54.6% and 69.3% of control, respectively. No differences were observed in the density of the inward rectifier K+ current (I(K1)), rapid delayed rectifier K+ current (I(Kr)), and L-type Ca2+ current (I(Ca)). Western blot analysis revealed a reduced expression of Kv4.3 and MinK (to 25+/-21% and 48+/-15% of control, respectively) in diabetic dogs, while other channel proteins were unchanged (HERG, MiRP1, alpha(1c)) or increased (Kv1.4, KChIP2, KvLQT1). Insulin substitution fully prevented the diabetes-induced changes in I(Ks), KvLQT1 and MinK, however, the changes in I(to), Kv4.3, and Kv1.4 were only partially diminished by insulin.
  
  
  CONCLUSIONS
  It is concluded that type 1 diabetes mellitus, although only moderately, lengthens ventricular repolarization, attenuates the repolarization reserve by decreasing I(to) and I(Ks) currents, and thereby may markedly enhance the risk of sudden cardiac death.
authors.affiliation: Department of Pharmacology and Pharmacotherapy, University of Szeged, Hungary.
authors.forename: Csaba
authors.initials: C
authors.lastname: Lengyel
authors.forename: László
authors.initials: L
authors.lastname: Virág
authors.forename: Tamás
authors.initials: T
authors.lastname: Bíró
authors.forename: Norbert
authors.initials: N
authors.lastname: Jost
authors.forename: János
authors.initials: J
authors.lastname: Magyar
authors.forename: Péter
authors.initials: P
authors.lastname: Biliczki
authors.forename: Erzsébet
authors.initials: E
authors.lastname: Kocsis
authors.forename: Réka
authors.initials: R
authors.lastname: Skoumal
authors.forename: Péter P
authors.initials: PP
authors.lastname: Nánási
authors.forename: Miklós
authors.initials: M
authors.lastname: Tóth
authors.forename: Valéria
authors.initials: V
authors.lastname: Kecskeméti
authors.forename: Julius Gy
authors.initials: JG
authors.lastname: Papp
authors.forename: András
authors.initials: A
authors.lastname: Varró
mesh_terms: D000200:Action Potentials; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D020746:Calcium Channels, L-Type / Q000378:metabolism; D051668:Delayed Rectifier Potassium Channels / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental; D003922:Diabetes Mellitus, Type 1 / Q000188:drug therapy / Q000378:metabolism* / Q000503:physiopathology*; D004285:Dogs; D004562:Electrocardiography; D005260:Female; D007328:Insulin / Q000627:therapeutic use; D051657:KCNQ1 Potassium Channel / Q000378:metabolism; D051665:Kv1.4 Potassium Channel / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D024661:Potassium Channels, Inwardly Rectifying / Q000378:metabolism; D051672:Shal Potassium Channels / Q000378:metabolism
chemical_list: D020746:Calcium Channels, L-Type; D051668:Delayed Rectifier Potassium Channels; D007328:Insulin; D051657:KCNQ1 Potassium Channel; D051665:Kv1.4 Potassium Channel; D024661:Potassium Channels, Inwardly Rectifying; D051672:Shal Potassium Channels