title: Proteomic analysis of proteins differentially expressed in preeclamptic trophoblasts.
abstract: OBJECTIVE
  To identify differential trophoblastic proteins associated with preeclampsia (PE) by proteomic analysis.
  
  
  METHODS
  We isolated and purified placental trophoblasts from normotensive pregnant women and patients with PE by a continuous Percoll gradient. The expression of proteins was determined by sliver staining after two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Proteins of interest were identified using matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS).
  
  
  RESULTS
  The overall trophoblastic protein expression patterns in preeclamptic and corresponding normotensive placentas were quite similar except for some areas. Of 34 differentially expressed protein spots (p < 0.05 by paired t-test), seven differential proteins from nine spots were identified by MALDI-TOF-MS. The expression of the following proteins was repressed (p < 0.01): disulfide isomerase ER-60, peroxiredoxin 2, and Delta3,5-Delta2,4-dienoyl-CoA isomerase. Four proteins (protein disulfide isomerase precursor, endoplasmic reticulum resident protein, dihydrolipoyl dehydrogenase and TIM21-like protein) were found to be significantly upregulated in PE (p < 0.01).
  
  
  CONCLUSIONS
  We identified several proteins with significant altered expression in PE using 2D-PAGE. This method is a powerful technique with which to search for not only quantitative but also qualitative changes in a biological process of interest.
authors.affiliation: Department of Gynecology and Obstetrics, First Affiliated Hospital of Nanjing Medical University, Nanjing Medical University, Nanjing, PR China. lizhou_sun121@hotmail.com
authors.forename: Li-zhou
authors.initials: LZ
authors.lastname: Sun
authors.forename: Na-na
authors.initials: NN
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: De
authors.forename: Yun-shan
authors.initials: YS
authors.lastname: Xiao
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis; D016022:Case-Control Studies; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D010449:Peptide Mapping; D011225:Pre-Eclampsia / Q000235:genetics / Q000473:pathology*; D011247:Pregnancy; D011336:Probability; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D012016:Reference Values; D012307:Risk Factors; D012494:Sampling Studies; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D014327:Trophoblasts / Q000166:cytology* / Q000502:physiology
chemical_list: D015415:Biomarkers; D011506:Proteins