Complete genome sequence of Haemophilus somnus (Histophilus somni) strain 129Pt and comparison to Haemophilus ducreyi 35000HP and Haemophilus influenzae Rd.
Haemophilus somnus can be either a commensal of bovine mucosal surfaces or an opportunistic pathogen. Pathogenic strains of H. somnus are a significant cause of systemic disease in cattle. We report the genome sequence of H. somnus 129Pt, a nonpathogenic commensal preputial isolate, and the results of a genome-wide comparative analysis of H. somnus 129Pt, Haemophilus influenzae Rd, and Haemophilus ducreyi 35000HP. We found unique genes in H. somnus 129Pt involved in lipooligosaccharide biosynthesis, carbohydrate uptake and metabolism, cation transport, amino acid metabolism, ubiquinone and menaquinone biosynthesis, cell surface adhesion, biosynthesis of cofactors, energy metabolism, and electron transport. There were also many genes in common among the three organisms. Our comparative analyses of H. somnus 129Pt, H. influenzae Rd, and H. ducreyi 35000HP revealed similarities and differences in the numbers and compositions of genes involved in metabolism, host colonization, and persistence. These results lay a foundation for research on the host specificities and niche preferences of these organisms. Future comparisons between H. somnus 129Pt and virulent strains will aid in the development of protective strategies and vaccines to protect cattle against H. somnus disease.
Challacombe
Jean F
JF
Department of Energy Joint Genome Institute, Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA. jchalla@lanl.gov
Duncan
A J
AJ
Brettin
Thomas S
TS
Bruce
David
D
Chertkov
Olga
O
Detter
J Chris
JC
Han
Cliff S
CS
Misra
Monica
M
Richardson
Paul
P
Tapia
Roxanne
R
Thayer
Nina
N
Xie
Gary
G
Inzana
Thomas J
TJ
D000596:Amino Acids / Q000378:metabolism; D001422:Bacterial Adhesion; D001483:Base Sequence; D002952:Citric Acid Cycle; D016680:Genome, Bacterial; D006191:Haemophilus ducreyi / Q000235:genetics* / Q000378:metabolism; D006193:Haemophilus influenzae / Q000235:genetics* / Q000378:metabolism; D044138:Haemophilus somnus / Q000235:genetics* / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism; D008969:Molecular Sequence Data; D009243:NAD / Q000096:biosynthesis; D009711:Nucleotides / Q000096:biosynthesis; D010427:Pentose Phosphate Pathway; D014168:Transferrin / Q000378:metabolism; D014451:Ubiquinone / Q000096:biosynthesis
D000596:Amino Acids; D006454:Hemoglobins; D009711:Nucleotides; D014168:Transferrin; D009243:NAD; D014451:Ubiquinone