[Study of Papaver somniferum cultivars identification by TD-RAPD technique].
OBJECTIVE
The feasibility of Papaver somniferum L. cultivars identification was explored by TD-RAPD technique.


METHODS
Genomic DNA was extracted by improved CTAB method. One sample of species from Papaver somniferum L in xishuangbanna area. was studied by using TD-RAPD method.


RESULTS
We established an optimal method of extracting genomic DNA. Six primers were picked out from 10 primers.


CONCLUSIONS
TD-RAPD could be applied to researches of molecular marker of Papaver somniferum L. TD-RAPD technique provide a method to constitute DNA database of Papaver somniferum L. and conclude the source of opium poppy.
Lu
Fan
F
Yunnan Criminal Science and Technology Institute, Kunming 650021, China.
Hong
Jun-Yan
JY
He
Rui
R
Li
Li-Sha
LS
D017931:DNA Primers; D018744:DNA, Plant / Q000032:analysis* / Q000302:isolation & purification; D005240:Feasibility Studies; D006801:Humans; D010207:Papaver / Q000235:genetics*; D018515:Plant Leaves / Q000235:genetics; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D013045:Species Specificity
D017931:DNA Primers; D018744:DNA, Plant