The M and N genes-based simplex and multiplex PCRs are better than the F or H gene-based simplex PCR for Peste-des-petits-ruminants virus.
Nucleocapsid (N), matrix (M) and hemagglutinin (H) genes-based simplex PCRs and an N and M genes-based multiplex PCR were developed for detection of Peste-des-petits-ruminants virus (PPRV). The M gene PCR was the most sensitive, followed by N, H and an already described fusion (F) gene PCRs, as they could detect the virus in samples with titers of 101, 102, 104and 105 TCID50/ml, respectively. The multiplex PCR was as sensitive as the M gene PCR, but it had the advantage of differentiating PPRV from Rinderpest virus (RPV).
George
A
A
Division of Virology, Indian Veterinary Research Institute, Mukteswar, Nainital, India.
Dhar
P
P
Sreenivasa
B P
BP
Singh
R P
RP
Bandyopadhyay
S K
SK
D004587:Electrophoresis, Agar Gel; D005814:Genes, Viral; D006389:Hemagglutinins, Viral / Q000235:genetics*; D019590:Nucleocapsid Proteins / Q000235:genetics*; D018107:Peste-des-petits-ruminants virus / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000235:genetics; D012302:Rinderpest virus / Q000235:genetics / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D014760:Viral Fusion Proteins / Q000235:genetics*; D014763:Viral Matrix Proteins / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
C096524:F protein, Peste-des-petits-ruminants virus; D006389:Hemagglutinins, Viral; D019590:Nucleocapsid Proteins; D012367:RNA, Viral; D014760:Viral Fusion Proteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins