Acute effects of an anatoxin-a producing cyanobacterium on juvenile fish-Cyprinus carpio L.
The worldwide increase of eutrophication in fresh water bodies has caused cyanobacterial blooms to be more frequent. Anatoxin-a is a potent neurotoxin known to be produced by several genera of cyanobacteria including Anabaena. In this work, we exposed juvenile carps to freeze-dried cells of a toxic strain of the cyanobacterium Anabaena sp. during a 4-day period. Two different cell density--10(5) and 10(7) cell ml(-1)--were assayed. Lethality and anatoxin-a concentration in the whole fish were determined. In the higher cell density, all fish died between 26 and 29 h after exposure to toxin, whereas in the 10(5) cell ml(-1) density no deaths were observed. Levels of anatoxin-a in the whole fish ranged between 0.031 microg g(-1)d.w. at the 10(5) cell ml(-1) concentration and 0.768 microg g(-1)d.w. at 10(7) cell ml(-1). Minor uptake of anatoxin-a occurred.
Osswald
Joana
J
Interdisciplinary Centre of Marine and Environmental Research-CIMAR-Laboratório Associado, Rua dos Bragas, 289, 4050 123 Porto, Portugal.
Rellán
Sandra
S
Carvalho
António Paulo
AP
Gago
Ana
A
Vasconcelos
Vitor
V
D017033:Anabaena / Q000737:chemistry*; D000818:Animals; D001822:Body Burden; D002347:Carps / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D000087522:Cyanobacteria Toxins; D004305:Dose-Response Relationship, Drug; D008387:Marine Toxins / Q000493:pharmacokinetics / Q000633:toxicity*; D018675:Toxicity Tests; D014326:Tropanes / Q000493:pharmacokinetics / Q000633:toxicity*
D000087522:Cyanobacteria Toxins; D008387:Marine Toxins; D014326:Tropanes; C509783:anatoxin a