Molecular characterization of pig ST8Sia IV--a critical gene for the formation of neural cell adhesion molecule and its response to sialic acid supplement in piglets.
ST8Sia IV (polysialyltransferase IV gene) encodes a key enzyme that is required for polysialic acid synthesis. Polysialic acid is a component of the neural cell adhesion molecule and is necessary for synaptic plasticity of neural cells. We characterized 5.3 kb of pig ST8Sia IV cDNA and determined its expression profile in different organs. In hippocampus, ST8Sia IV mRNA levels were increased approximately 4.5-fold in piglets with sialic acid as a milk supplement, which suggested that exogenous sialic acid is a conditionally essential nutrient for early brain development. Extensive analyses were also performed among its orthologs from human, mouse, rat, chicken, frog and zebrafish. Our results supported that the piglet is a better animal model than other nonprimate species in the studies of ST8Sia IV related metabolism and nutrition in human infants. This pig cDNA provides a basis for uncovering the roles of ST8Sia IV during piglet development and maturation.
Wang
Bing
B
Human Nutrition Unit, School of Molecular and Microbial Biosciences, University of Sydney, Sydney, NSW, Australia.
Hu
Honghua
H
Yu
Bing
B
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D017931:DNA Primers; D019587:Dietary Supplements; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid / Q000493:pharmacokinetics*; D019006:Neural Cell Adhesion Molecules / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D012799:Sialyltransferases / Q000235:genetics*; D013552:Swine
D017931:DNA Primers; D019006:Neural Cell Adhesion Molecules; D012799:Sialyltransferases; C517012:ST8Sia IV protein, swine; D019158:N-Acetylneuraminic Acid