[Structural characterization of tissue-specific galactan from flax fibers by 1H NMR and MALDI TOF mass spectrometry].
A high-molecular-mass polysaccharide galactan (M 2000 kDa) was isolated from flax at the stage of cell wall thickening of the bast fiber development. The polymer structure was studied by 1H NMR spectroscopy and MALDI TOF mass spectrometry. It is built up of Gal (59%), Rha (15%), GalA (23%), and Ara (3%) residues. The galactan backbone consists of successively alternating monomer disaccharide units (--> 4GalA1 --> 2Rha1 -->)n and is similar in its structure to the backbone of rhamnogalacturonan-1 (RG-I). Rhamnose residues bear in position 4 beta-(1 --> 4)-galactose side chains of various lengths with a polymerization degree of up to 28 or higher. A part of the side chains have branchings.
Gur'ianov
O
O
Gorshkova
T
T
Kabel
M
M
Schols
H
H
van Dam
J E G
JE
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D019597:Flax / Q000737:chemistry*; D005685:Galactans / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D005685:Galactans