title: Chemoprevention of aberrant crypt foci in the colon of rats by dietary onion.
abstract: Onion intake might reduce the risk of colorectal cancer, according to epidemiology. However, Femia showed in 2003 that diets with a 20% onion intake increase carcinogenesis in rats. We speculated this dose was too high. Prevention of initiation was thus tested in 60 rats given a 5% dried onion diet or AIN76 diet, and initiated 12 days later with azoxymethane (AOM, 1x20 mg/kg i.p.), 2-amino-3-methylimidazo[4,5-f]quinoline (IQ, 2x200 mg/kg p.o.), or N-nitroso-N-methylurea (2x50 mg/kg p.o.). Prevention of promotion was tested in 38 rats given AOM, then randomised to: AIN76 diet; 5% onion diet; phytochemicals diet (supplemented with propyl-disulfide, quercetine-glycosides and oligofructose); 1% pluronic F68 diet (a potent chemopreventive PEG-like block-polymer, used as a positive control). Aberrant crypt foci (ACF) were scored 30 days (initiation) or 100 days (promotion) after carcinogen injection. The onion diet given during initiation reduced the number of AOM-induced ACF (60 versus 86, p=0.03), and the size of IQ-induced ACF (1.33 versus 1.97, p=0.02). Given post-initiation, the onion diet reduced the number of ACF (34 versus 59, p=0.008) and of large ACF (6 versus 15, p=0.02). Phytochemicals diet and pluronic diet reduced ACF growth similarly. Data show that a 5% onion diet reduced carcinogenesis during initiation and promotion stages, and suggest this chemoprevention is due to known phytochemicals.
authors.affiliation: UMR1089 Xénobiotiques, INRA & ENVT, 23 ch. Capelles, F-31076 Toulouse, France.
authors.forename: Sylviane
authors.initials: S
authors.lastname: Taché
authors.forename: Aélis
authors.initials: A
authors.lastname: Ladam
authors.forename: Denis E
authors.initials: DE
authors.lastname: Corpet
mesh_terms: D000818:Animals; D001397:Azoxymethane / Q000633:toxicity; D002273:Carcinogens / Q000633:toxicity; D018890:Chemoprevention / Q000379:methods*; D003110:Colonic Neoplasms / Q000178:diet therapy*; D005260:Female; D019697:Onions; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D002273:Carcinogens; D001397:Azoxymethane