Innate immune function of the human decidual cell at the maternal-fetal interface.
The aim of the study was to investigate the innate immune function of decidual cells from term unlabored pregnancies by determining Toll-like receptor presence and function. Using immunohistochemistry, reverse transcriptase polymerase chain reaction and NF-kappaB-luciferase plasmid transfection, Toll-like receptor presence and function was determined. Decidual cells express Toll-like receptors 1, 2, 4 and 6 which respond to lipopolysaccharide and peptidoglycan stimulation producing Interleukin-8. Decidual cells from women at term have innate immune function and are capable of producing Interleukin responses to bacterial ligands.
Canavan
Timothy P
TP
University of Pittsburgh School of Medicine, Magee Womens Hospital, Department of Obstetrics, Gynecology and Reproductive Sciences, 300 Halket Street, Pittsburgh, PA 15213, USA. timothyc@greenhosp.org
Simhan
Hyagriv N
HN
D003656:Decidua / Q000166:cytology / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007113:Immunity, Innate; D007150:Immunohistochemistry; D016209:Interleukin-8 / Q000276:immunology* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology*; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D010457:Peptidoglycan / Q000276:immunology*; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051193:Toll-Like Receptors / Q000276:immunology / Q000378:metabolism* / Q000502:physiology; D014162:Transfection
D016209:Interleukin-8; D008070:Lipopolysaccharides; D016328:NF-kappa B; D010457:Peptidoglycan; D051193:Toll-Like Receptors