title: [Effects of metoclopramide-induced hyperprolactinemia on the murine corneal].
abstract: OBJECTIVE
  To evaluate the morphological changes in murine cornea upon metoclopramide-induced hyperprolactinemia during the proestrous phase or pregnancy.
  
  
  METHODS
  Forty adult mice were divided into two groups: (control) CTR1 and (treated with metoclopramide (MET1). After fifty days, half of the mice were sacrificed. The remaining animals were mated, and then labeled as pregnant controls (CTR2). Part of these animals were treated with metoclopramide and constituted the metoclopramide-treated pregnant (MET2) group. The groups CTR2 and MET2 were sacrificed on the 6th day of pregnancy. The hormonal levels were assessed by chemioluminescence and radioimmunoassay methods and the cornea was removed for the histomorphometric study.
  
  
  RESULTS
  The epithelial, stromal, endothelial and total thickness in the experimental group was: MET1 and MET2 were higher than one in the control group: CTR1 and CTR2. There was a significant reduction of the hormonal level in the animals that received metoclopramide as compared to controls (CTR1: estradiol = 156.6 +/- 42.2 pg/ml; progesterone = 39.4 +/- 5.1 ng/ml; prolactin = 130.4 +/- 26.2 ng/ml; MET1: estradiol = 108.0 +/- 33.1 pg/ml; progesterone = 28.0 +/- 6.4 ng/ml; prolactin = 551.5 +/- 23.3 ng/ml; CTR2: estradiol = 354.0 +/- 56.0 pg/ml; progesterone = 251.0 +/- 56.0 ng/ml; prolactin = 423.2 +/- 28.1 ng/ml; MET2: estradiol = 293.0 +/- 43.0 pg/ml; progesterone = 184.0 +/- 33.0 ng/ml; prolactin = 823.1 +/- 51.1 ng/ml).
  
  
  CONCLUSIONS
  The metoclopramide-induced hyperprolactinemia may increase corneal layers, mainly in pregnant mice. Possibly, this effect is related to reduction in estrogen and progesterone production.
authors.affiliation: Departamento de Ginecologia, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors.forename: Carina
authors.initials: C
authors.lastname: Verna
authors.forename: José Maria Júnior
authors.initials: JM
authors.lastname: Soares
authors.forename: Fernanda Watanabe
authors.initials: FW
authors.lastname: Martins
authors.forename: Regina Célia
authors.initials: RC
authors.lastname: Teixeira
authors.forename: Rejane
authors.initials: R
authors.lastname: Mosquette
authors.forename: Ricardo Santos
authors.initials: RS
authors.lastname: Simões
authors.forename: Manuel de Jesus
authors.initials: Mde J
authors.lastname: Simões
authors.forename: Edmund Chada
authors.initials: EC
authors.lastname: Baracat
mesh_terms: D000818:Animals; D001082:Aqueous Humor; D003315:Cornea / Q000187:drug effects* / Q000473:pathology; D004195:Disease Models, Animal; D018492:Dopamine Antagonists / Q000633:toxicity*; D004958:Estradiol / Q000097:blood; D005260:Female; D042341:Gonadal Hormones / Q000097:blood*; D006651:Histocytochemistry; D006966:Hyperprolactinemia / Q000139:chemically induced / Q000473:pathology*; D007091:Image Processing, Computer-Assisted; D008787:Metoclopramide / Q000633:toxicity*; D051379:Mice; D011247:Pregnancy; D011359:Proestrus / Q000187:drug effects*; D011374:Progesterone / Q000097:blood; D011388:Prolactin / Q000097:blood
chemical_list: D018492:Dopamine Antagonists; D042341:Gonadal Hormones; D011374:Progesterone; D004958:Estradiol; D011388:Prolactin; D008787:Metoclopramide