title: Optimization of the methods for introduction of amine groups onto the silica nanoparticle surface.
abstract: The luminescent silica nanoparticle has attracted the researchers' concentration in bioanalysis recently. Its extensive application is based on the immobilization of various biomolecules such as deoxyribonucleic acid, antibody, and so forth onto the surface. By comparing different introduction methods of amine groups, it was confirmed that the "two-step" route is more preferable by adding tetraethyl orthosilicate and 3-aminopropyl-(triethoxyl)silane in sequence, to attain ideal amine-modified silica nanoparticles. On this basis, carboxyl groups were derived from amine groups on the nanoparticle surface and then were activated by 1-ethyl-3-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxy-succinimide. Finally, mouse monoclonal antihuman CD71 antibody (McAb CD71) and transferrin were effectively linked with the carboxyl groups and successfully labeled the receptors in the membrane of fibroblast cells, respectively.
authors.affiliation: Key Laboratory of Biomedical Photonics of Ministry of Education-Hubei Bioinformatics, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, HuBei 430074, People's Republic of China.
authors.forename: Song
authors.initials: S
authors.lastname: Liu
authors.forename: Hai-Li
authors.initials: HL
authors.lastname: Zhang
authors.forename: Tian-Cai
authors.initials: TC
authors.lastname: Liu
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.forename: Yuan-Cheng
authors.initials: YC
authors.lastname: Cao
authors.forename: Zhen-Li
authors.initials: ZL
authors.lastname: Huang
authors.forename: Yuan-Di
authors.initials: YD
authors.lastname: Zhao
authors.forename: Qing-Ming
authors.initials: QM
authors.lastname: Luo
mesh_terms: D000588:Amines / Q000737:chemistry*; D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000276:immunology; D005347:Fibroblasts / Q000737:chemistry / Q000166:cytology; D006801:Humans; D008722:Methods; D051379:Mice; D015336:Molecular Probe Techniques; D053758:Nanoparticles / Q000737:chemistry*; D011990:Receptors, Transferrin / Q000276:immunology; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties; D014168:Transferrin
chemical_list: D000588:Amines; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; C068126:CD71 antigen; D011990:Receptors, Transferrin; D014168:Transferrin; D012822:Silicon Dioxide