Formation of a new oxidative metabolite from kobophenol A by human intestinal bacterium Klebsiella pneumoniae.
During our studies on the metabolism of kobophenol A (1) in rats, we had isolated, purified, and identified the main new oxidative metabolite of 1, koboquinone A (2) from rats' feces. To elucidate the metabolic pathway of 1 in rats, we conducted the in vitro metabolic experiments of 1 by human intestinal bacteria and found that Klebsiella pneumoniae produced appreciable amounts of 2. This was verified by means of high performance liquid chromatography mass/mass spectrometry (HPLC/MS/MS) analysis.
Liang
Gaolin
G
Department of Chemistry of Natural Drugs, School of Pharmacy, Fudan University, Shanghai 200032, People's Republic of China. lianggl72@shmu.edu.cn
Qian
Lisheng
L
Cui
Yiling
Y
Hu
Changqi
C
D016227:Benzoquinones / Q000737:chemistry / Q000378:metabolism*; D032602:Caragana / Q000737:chemistry; D041981:Gastrointestinal Tract / Q000382:microbiology; D006801:Humans; D007711:Klebsiella pneumoniae / Q000378:metabolism*; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D013267:Stilbenes / Q000737:chemistry / Q000378:metabolism*
D016227:Benzoquinones; D013267:Stilbenes; C510380:kobophenol A; C499874:koboquinone A