Detection of giardine gene in local isolates of Giardia duodenalis by polymerase chain reaction (PCR).
Giardia duodenalis is an intestinal parasite that causes diarrhoea and malabsorption in children. The parasite also infects AIDS patients with a weak immune system. A study was carried out on six local isolates of Giardia duodenalis (110, 7304, 6304, M007, 2002 and 6307) from faeces of Orang Asli patients admitted to the Gombak Hospital. WB, a reference pathogenic strain from human and G. muris from a wild mouse, were commercially obtained from the American Type Culture Collection (ATCC). All the isolates were cultured axenically in TYI-S-33 medium. Two sets of primers were used for the techniques: primers LP1 and RP1 and primers LP2 and RP2. The sets of primers amplified giardine gene of 171 bp and 218 bp in sizes respectively. The study showed that the two sets of primers could detect G. duodenalis to the genus and species level specifically.
Latifah
I
I
Biotechnology Unit, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia. Latifah@imr.gov.my
Teoh
K Y
KY
Wan
K L
KL
Rahmah
M
M
Normaznah
Y
Y
Rohani
A
A
D000818:Animals; D017931:DNA Primers; D016054:DNA, Protozoan / Q000032:analysis*; D017125:Genes, Protozoan; D016829:Giardia lamblia / Q000235:genetics* / Q000302:isolation & purification*; D005873:Giardiasis / Q000175:diagnosis; D006801:Humans; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods*; D015800:Protozoan Proteins / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis; D012680:Sensitivity and Specificity
D017931:DNA Primers; D016054:DNA, Protozoan; D015800:Protozoan Proteins; D012333:RNA, Messenger