title: Methionine-rich storage protein gene in the rice stem borer, Chilo suppressalis, is expressed during diapause in response to cold acclimation.
abstract: Gene expressions of acclimatized and non-acclimatized diapausing larvae were examined in Chilo suppressalis using a subtraction technique. A gene encoding a methionine-rich storage protein, CsSP1, was cloned and its complete cDNA sequence was determined. Potentially, CsSP1 encoded a 758-amino acid protein, with a calculated molecular weight of 88.8 kDa. The expression level of CsSP1 was higher in nondiapausing larvae than in diapausing ones. The CsSP1 expression was up-regulated in diapausing larvae when the temperature of cold acclimation was shifted to 5 degrees C. The up-regulated level was maintained at 40 days after incubation at 5 degrees C. In nondiapausing larvae, CsSP1 expression was down-regulated when the temperature was below developmental zero. Involvement of CsSP1 in diapause, cold tolerance acquisition and postdiapause development in C. suppressalis is discussed.
authors.affiliation: Research Institute for Bioresources, Okayama University, Kurashiki, Okayama, Japan. sonodas@rib.okayama-u.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Sonoda
authors.forename: K
authors.initials: K
authors.lastname: Fukumoto
authors.forename: Y
authors.initials: Y
authors.lastname: Izumi
authors.forename: M
authors.initials: M
authors.lastname: Ashfaq
authors.forename: H
authors.initials: H
authors.lastname: Yoshida
authors.forename: H
authors.initials: H
authors.lastname: Tsumuki
mesh_terms: D000064:Acclimatization; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003080:Cold Temperature; D005260:Female; D005786:Gene Expression Regulation; D017344:Genes, Insect / Q000235:genetics*; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics; D007814:Larva / Q000254:growth & development / Q000378:metabolism; D007915:Lepidoptera / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D008675:Metamorphosis, Biological / Q000235:genetics*; D008715:Methionine / Q000032:analysis; D008969:Molecular Sequence Data; D012275:Oryza / Q000469:parasitology*; D018547:Plant Stems / Q000469:parasitology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D019476:Insect Proteins; D012333:RNA, Messenger; D008715:Methionine