title: Transcriptome of local innate and adaptive immunity during early phase of infectious bronchitis viral infection.
abstract: To understand the mechanistic basis of local innate and adaptive immunity against infectious bronchitis virus (IBV) at the molecular level, we examined the gene transcription profile of tracheal epithelial layers 3 d after infection of chickens with an attenuated IBV-Massachusetts strain. Results suggested that the transcription levels of 365 genes were either upregulated or downregulated (2-fold and higher) after IBV infection. Among the upregulated 250 genes, 25 were directly immune-related genes. These upregulated immune response genes included TLR2, TLR3, interferon-induced antiviral genes (Mx), and genes responsible for cytotoxic T cell killing such as Fas antigen and granzyme-A. Overall, a diversity of innate immunity and helper T cell type 1 (Th1)-biased adaptive immunity are activated in the host's early defense against IBV invasion, and they are responsible for the rapid clearance of virus from the local infection.
authors.affiliation: Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57007, USA. xiuqing.wang@sdstate.edu
authors.forename: Xiuqing
authors.initials: X
authors.lastname: Wang
authors.forename: Artur J M
authors.initials: AJ
authors.lastname: Rosa
authors.forename: Henrique N
authors.initials: HN
authors.lastname: Oliverira
authors.forename: Guilherme J M
authors.initials: GJ
authors.lastname: Rosa
authors.forename: Xueshui
authors.initials: X
authors.lastname: Guo
authors.forename: Mark
authors.initials: M
authors.lastname: Travnicek
authors.forename: Theodore
authors.initials: T
authors.lastname: Girshick
mesh_terms: D000818:Animals; D002645:Chickens; D018352:Coronavirus Infections / Q000276:immunology*; D004848:Epithelium / Q000276:immunology / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D053804:Granzymes / Q000235:genetics / Q000378:metabolism; D007110:Immunity, Active; D007113:Immunity, Innate; D001351:Infectious bronchitis virus; D007372:Interferons / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D014132:Trachea / Q000276:immunology; D019014:fas Receptor / Q000235:genetics / Q000378:metabolism
chemical_list: D019014:fas Receptor; D007372:Interferons; D053804:Granzymes