Ischemia promotes calpain-mediated degradation of p120-catenin in SH-SY5Y cells.
p120-catenin contributes to the cadherin-mediated adhesion and aggregation of cells. mu-Calpain was activated and p120-catenin was degraded after 36 h of ischemia in differentiated SH-SY5Y cells. Calpain inhibitors Cbz-Val-Phe-H (MDL28170, 20 microM) and N-acetyl-leucyl-leucyl-norleucinal (ALLN, 20 microM) increased the levels of dephosphorylated p120-catenin, aggregation, and cell survival as detected by reduced LDH release in ischemic cells. However, a proteasome inhibitor lactacystin had no such effects. This is the first report of the calpain-mediated degradation of p120-catenin and an association between the level of dephosphorylated p120-catenin and cell aggregation in ischemic neuronal cells.
Ohno
Hiroshi
H
Department of Forensic Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
Uemura
Koichi
K
Shintani-Ishida
Kaori
K
Nakamura
Mihoko
M
Inomata
Mitsushi
M
Yoshida
Ken-ichi
K
D000111:Acetylcysteine / Q000031:analogs & derivatives / Q000494:pharmacology; D002154:Calpain / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051177:Catenins; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002449:Cell Aggregation / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D004151:Dipeptides / Q000494:pharmacology; D006801:Humans; D007511:Ischemia / Q000503:physiopathology*; D007976:Leupeptins / Q000494:pharmacology; D009447:Neuroblastoma / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism*; D000095002:Delta Catenin
D051177:Catenins; D015815:Cell Adhesion Molecules; D004151:Dipeptides; D007976:Leupeptins; D010750:Phosphoproteins; C063261:acetylleucyl-leucyl-norleucinal; C067713:lactacystin; D002154:Calpain; C058076:calpain inhibitor III; D000111:Acetylcysteine; D000095002:Delta Catenin; C000722347:CTNND1 protein, human