title: Reduced apoptosis in human intestinal cells cured of persistent poliovirus infection.
abstract: Cells cured of persistent virus infection can be used to investigate cellular pathways of resistance to viral cytopathic effects. Persistent poliovirus (PV) infections were established in human intestinal Caco-2 cells, and spontaneously cured cell cultures were obtained. Two cell clones, cl6 and b13, cured of type 3 PV mutant infection and their parental Caco-2 cells were compared for susceptibility to PV infection, PV receptor CD155 expression, capacity to differentiate into polarized enterocytes, and PV-, staurosporine-, and actinomycin D-induced apoptosis. Our results strongly suggest that cells that are partially resistant to apoptosis can be selected during persistent virus infection.
authors.affiliation: Virus Entérotropes et Stratégies Antivirales, Institut Pasteur, 25 rue du Dr. Roux, 75724 Paris Cedex 15, France.
authors.forename: Karine
authors.initials: K
authors.lastname: Labadie
authors.forename: Aure
authors.initials: A
authors.lastname: Saulnier
authors.forename: Sandra
authors.initials: S
authors.lastname: Martin-Latil
authors.forename: Florence
authors.initials: F
authors.lastname: Colbère-Garapin
mesh_terms: D017209:Apoptosis; D018938:Caco-2 Cells; D002454:Cell Differentiation / Q000187:drug effects; D002908:Chronic Disease; D002999:Clone Cells / Q000821:virology; D003609:Dactinomycin / Q000494:pharmacology; D004198:Disease Susceptibility; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D007422:Intestines / Q000166:cytology / Q000821:virology*; D008565:Membrane Proteins / Q000378:metabolism; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D011051:Poliomyelitis / Q000821:virology*; D017955:Poliovirus / Q000235:genetics / Q000502:physiology*; D011991:Receptors, Virus / Q000378:metabolism; D019311:Staurosporine / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D008565:Membrane Proteins; D019384:Nucleic Acid Synthesis Inhibitors; D011991:Receptors, Virus; C066100:poliovirus receptor; D003609:Dactinomycin; D019311:Staurosporine