title: mRNA expression and RNA editing (2451 C-to-U) of IL-12 receptor beta2 in adult atopic patients.
abstract: Interleukin (IL)-12 activates T helper (Th) 1 cells to produce interferon (IFN)-gamma which inhibits atopic inflammation. IL-12 acts through interaction with its receptor, especially beta(2) subunit. In several studies, the low production of IFN-gamma in peripheral mononuclear cells of atopic patients on response to IL-12 stimulation has been reported. Therefore we investigated the IL-12 receptor beta(2) (IL-12R beta(2)) mRNA expression and RNA editing, nucleotide 2451 C-to-U conversion, to find the cause of low responsiveness to IL-12 in atopy. Quantitative real time PCR for mRNA expression and sequence analysis for RNA editing were performed in 80 atopic patients and 54 healthy controls. The expression of IL-12R beta(2) mRNA was significantly lower in atopic patients than healthy controls (p<0.05). In sequence analysis, RNA editing on nucleotide 2451 was not found from either atopic patients or healthy controls. In additional evaluation, there was no relationship between expression of IL-12R beta(2) mRNA and serum total IgE or blood eosinophil count. Reduced IL-12R beta(2) mRNA expression in atopic patients indicate the reduced capacity to respond to IL-12 which induce IFN-gamma production and this may contribute to Th2-skewed immune response in atopy.
authors.affiliation: Daegu Kyoungbook Blood Center, Korea Red Cross, Daegu, Korea.
authors.forename: Eun Jin
authors.initials: EJ
authors.lastname: Kim
authors.forename: Won Mok
authors.initials: WM
authors.lastname: Lee
authors.forename: Jung Sook
authors.initials: JS
authors.lastname: Ha
authors.forename: Nam Hee
authors.initials: NH
authors.lastname: Ryoo
authors.forename: Dong Suk
authors.initials: DS
authors.lastname: Jeon
authors.forename: Jae Ryong
authors.initials: JR
authors.lastname: Kim
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D005260:Female; D020022:Genetic Predisposition to Disease / Q000453:epidemiology / Q000235:genetics; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000453:epidemiology* / Q000235:genetics* / Q000378:metabolism; D007723:Korea / Q000453:epidemiology; D008297:Male; D017393:RNA Editing / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*; D053707:Receptors, Interleukin-12 / Q000235:genetics* / Q000378:metabolism; D015203:Reproducibility of Results; D018570:Risk Assessment / Q000379:methods*; D012307:Risk Factors; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers; C508439:IL12RB2 protein, human; D012333:RNA, Messenger; D053707:Receptors, Interleukin-12