Sensitive protein detection via triple-binder proximity ligation assays.
The detection of weakly expressed proteins and protein complexes in biological samples represents a fundamental challenge. We have developed a new proximity-ligation strategy named 3PLA that uses three recognition events for the highly specific and sensitive detection of as little as a hundred molecules of the vascular endothelial growth factor (VEGF), the biomarkers troponin I, and prostate-specific antigen (PSA) alone or in complex with an inhibitor--demonstrating the versatility of 3PLA.
Schallmeiner
Edith
E
Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Se-75185 Uppsala, Sweden. edith.schallmeiner@genpat.uu.se
Oksanen
Elli
E
Ericsson
Olle
O
Spångberg
Lena
L
Eriksson
Susann
S
Stenman
Ulf-Håkan
UH
Pettersson
Kim
K
Landegren
Ulf
U
D000906:Antibodies / Q000378:metabolism; D001710:Biotin / Q000378:metabolism; D006801:Humans; D007118:Immunoassay; D015336:Molecular Probe Techniques; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000378:metabolism; D016133:Polymerase Chain Reaction; D017430:Prostate-Specific Antigen / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D012680:Sensitivity and Specificity; D019809:Streptavidin / Q000378:metabolism; D019210:Troponin I / Q000032:analysis; D020298:U937 Cells; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis
D000906:Antibodies; D009841:Oligonucleotides; D011506:Proteins; D019210:Troponin I; D042461:Vascular Endothelial Growth Factor A; D001710:Biotin; D019809:Streptavidin; D017430:Prostate-Specific Antigen