[Effect of exogenous dna injection on leukopoietic repair and antitumor action of cyclophosphamide].
The cytostatic drug cyclophosphamide (CPA) in high dosage suppressed hemopoiesis by causing multiple double-strand breaks to occur in hemopoietic cell DNA and leading to mutation, chromosomal abberations and finally cell death. We tested fragmented DNA drugs for an ability of CPA to protect murine leukopoiesis on an assumption that once exogenous fragmented DNA had infiltrated into a cell, it might integrate with chromosomal DNA through homologous recombinations thus repairing damaged segments. DNA drugs did promote repair of leukocyte count in murine peripheral blood with leukopoiesis being suppressed by CPA administration. The levels of DNA derived from murine organs and human placenta were higher than those from salmon roe. Tumor growth was significantly inhibited following injection of placental DNA into mice bearing intramuscularly transplanted lymphosarcoma. Antitumor effect of combined CPA and DNA treatment was much higher than after CPA alone.
Nikolin
V P
VP
Popova
N A
NA
Sebeleva
T E
TE
Strunkin
D N
DN
Rogachev
V A
VA
Semenov
D V
DV
Bogachev
S S
SS
Iakubov
L A
LA
Shurdov
M A
MA
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D003520:Cyclophosphamide / Q000494:pharmacology*; D004247:DNA / Q000008:administration & dosage / Q000494:pharmacology*; D019891:Leukopoiesis / Q000187:drug effects*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA
D000970:Antineoplastic Agents; D003520:Cyclophosphamide; D004247:DNA