title: Tissue factor-positive microparticles: cellular origin and association with coagulation activation in patients with colorectal cancer.
abstract: The pathogenesis of hypercoagulability in cancer is not entirely understood. We hypothesized that in cancer patients circulating tissue factor-positive microparticles (TF (+) MPs) are increased and associated with hemostatic system activation. In 20 patients with advanced colorectal cancer and in 20 age- and sex-matched controls, number and cellular origin of TF (+) MPs were determined in plasma by flow cytometry. D-dimer was determined as an indicator of hemostatic system activation. Compared to controls, the median (interquartile range) number of TF (+) MPs was two-fold higher in cancer patients: 25.9 (15.4 - 42.0) x 10 (3) /ml plasma versus 13.1 (11.9 - 19.7) x 10 (3) /ml plasma, p = 0.007. This was mainly due to a higher amount of TF (+) MPs from platelets (13.4 [5.0 - 17.4] x 10 (3) /ml plasma vs. 5.8 [4.5 - 7.5] x 10 (3) /ml plasma, p = 0.017). TF (+) MPs correlated with D-dimer ( ? = 0.48, p = 0.002). High levels of TF (+) MPs in cancer patients and their correlation with D-dimer suggest that TF (+) MPs might be involved in hemostasis activation in cancer patients.
authors.affiliation: Dept. of Internal Medicine I, Medical University Vienna, Austria.
authors.forename: Gregor
authors.initials: G
authors.lastname: Hron
authors.forename: Marietta
authors.initials: M
authors.lastname: Kollars
authors.forename: Heinz
authors.initials: H
authors.lastname: Weber
authors.forename: Verena
authors.initials: V
authors.lastname: Sagaster
authors.forename: Peter
authors.initials: P
authors.lastname: Quehenberger
authors.forename: Sabine
authors.initials: S
authors.lastname: Eichinger
authors.forename: Paul A
authors.initials: PA
authors.lastname: Kyrle
authors.forename: Ansgar
authors.initials: A
authors.lastname: Weltermann
mesh_terms: D001777:Blood Coagulation; D002462:Cell Membrane / Q000737:chemistry*; D015179:Colorectal Neoplasms / Q000097:blood* / Q000150:complications; D005260:Female; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis; D005434:Flow Cytometry; D006487:Hemostasis; D006801:Humans; D008297:Male; D008875:Middle Aged; D010316:Particle Size; D019851:Thrombophilia / Q000209:etiology*; D013925:Thromboplastin / Q000032:analysis*
chemical_list: D005338:Fibrin Fibrinogen Degradation Products; C036309:fibrin fragment D; D013925:Thromboplastin