Detection of adenovirus DNA in throat swabs and blood by SYBR green real-time PCR assay in patients with adenovirus-associated tonsillitis.
The adenovirus DNA load ranged from 10(5) to 10(10) copy/mL and from 10(4) to 10(7) copy/mL in throat swabs and blood from patients with adenovirus-associated exudative tonsillitis, respectively. The copy number of adenovirus DNA in blood was positively correlated with the duration of the fever.
Kohdera
Urara
U
Nakano Children's Hospital, Osaka 535-0022, Japan. kohdera@nakano-kodomo.or.jp
Kino
Minoru
M
Ito
Masahiro
M
D000258:Adenovirus Infections, Human / Q000175:diagnosis / Q000821:virology; D000260:Adenoviruses, Human / Q000235:genetics / Q000302:isolation & purification*; D052160:Benzothiazoles; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis / Q000097:blood*; D003959:Diamines; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D007223:Infant; D008297:Male; D009930:Organic Chemicals; D010614:Pharynx / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011804:Quinolines; D013048:Specimen Handling / Q000379:methods; D014069:Tonsillitis / Q000175:diagnosis* / Q000821:virology*
D052160:Benzothiazoles; D004279:DNA, Viral; D003959:Diamines; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I