title: Strain-typing of Lentinula edodes in China with inter simple sequence repeat markers.
abstract: To validate strain typing by inter simple sequence repeat (ISSR) analysis in Lentinula edodes cultivars, 17 Chinese L. edodes strains including 15 cultivated strains cultivated on a large scale and two wild strains were analyzed with the ISSR technique. With the use of two ISSR primers, a total of 32 DNA products were detected, of which, 31 DNA products (96.9% of the detected products) were polymorphic between two or more strains. The profiles of those two primers could be employed to differentiate all of the tested strains. A cluster analysis based on ISSR data revealed that the 17 strains could be classified into two distinct groups. One group consisted of eight strains in which the cultivated strains were H (high-temperature)-type or B (broad-temperature)-type, and the other group comprised cultivated strains that were of the L (low-temperature)-type or M (medium-temperature)-type. In contrast to the two wild strains, the genetic diversity of 15 cultivated strains was very rich based on a similarity coefficient analysis.
authors.affiliation: Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Ruiying
authors.initials: R
authors.lastname: Zhang
authors.forename: Chenyang
authors.initials: C
authors.lastname: Huang
authors.forename: Suyue
authors.initials: S
authors.lastname: Zheng
authors.forename: Jinxia
authors.initials: J
authors.lastname: Zhang
authors.forename: Tzi Bun
authors.initials: TB
authors.lastname: Ng
authors.forename: Ruibo
authors.initials: R
authors.lastname: Jiang
authors.forename: Xuemei
authors.initials: X
authors.lastname: Zuo
authors.forename: Hexiang
authors.initials: H
authors.lastname: Wang
mesh_terms: D002681:China; D004271:DNA, Fungal / Q000032:analysis / Q000302:isolation & purification; D005819:Genetic Markers; D014644:Genetic Variation; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D020942:Shiitake Mushrooms / Q000145:classification* / Q000235:genetics* / Q000254:growth & development; D013045:Species Specificity
chemical_list: D004271:DNA, Fungal; D005819:Genetic Markers