Dynamic changes of gangliosides expression during the differentiation of embryonic and mesenchymal stem cells into neural cells.
Stem cells are used for the investigation of developmental processes at both cellular and organism levels and offer tremendous potentials for clinical applications as an unlimited source for transplantation. Gangliosides, sialic acid-conjugated glycosphingolipids, play important regulatory roles in cell proliferation and differentiation. However, their expression patterns in stem cells and during neuronal differentiation are not known. Here, we investigated expression of gangliosides during the growth of mouse embryonic stem cells (mESCs), mesenchymal stem cells (MSCs) and differentiated neuronal cells by using high-performance thin-layer chromatography (HPTLC). Monosialoganglioside 1 (GM1) was expressed in mESCs and MSCs, while GM3 and GD3 were expressed in embryonic bodies. In the 9-day old differentiated neuronal cells from mESCs cells and MSCs, GM1 and GT1b were expressed. Results from immunostaining were consistent with those observed by HPTLC assay. These suggest that gangliosides are specifically expressed according to differentiation of mESCs and MSCs into neuronal cells and expressional difference of gangliosides may be a useful marker to identify differentiation of mESCs and MSCs into neuronal cells.
Kwak
Dong-Hoon
DH
Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan 570-749, Korea.
Yu
Kweon
K
Kim
Sung-Min
SM
Lee
Dea-Hoon
DH
Kim
Sun-Mi
SM
Jung
Ji-Ung
JU
Seo
Jung-Woo
JW
Kim
Nari
N
Lee
Seoul
S
Jung
Kyu-Yong
KY
You
Hyung-Keun
HK
Kim
Hyun-A
HA
Choo
Young-Kug
YK
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D005732:Gangliosides / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*
D005732:Gangliosides