Application of bovine microsatellite markers for genetic diversity analysis of European bison (Bison bonasus).
In this study, the cross-amplification of a commercial multiplex set of 11 cattle (Bos taurus) microsatellites was tested on a panel of 35 European bison (Bison bonasus) individuals. After polymerase chain reaction optimization, all loci cross-amplified successfully in investigated bisons. Number of alleles and observed and expected heterozygosity per locus are in the range of 2-4, 0.086-0.629 and 0.288-0.621 respectively. The availability of a heterologous set of multiplexed microsatellite markers derived from cattle opens an avenue for collecting profound genetic data for efficient conservation management strategies of the European bison.
Roth
T
T
Institute of Veterinary Medicine, Georg-August-University of Göttingen, Göttingen, Germany.
Pfeiffer
I
I
Weising
K
K
Brenig
B
B
D000818:Animals; D016164:Bison / Q000235:genetics*; D002417:Cattle / Q000235:genetics*; D014644:Genetic Variation; D005858:Germany; D006197:Hair / Q000737:chemistry; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction