title: Effect of smoking on skin elastic fibres: morphometric and immunohistochemical analysis.
abstract: BACKGROUND
  It has been established during recent years that smoking is an independent risk factor for the development of premature facial wrinkling. The underlying mechanism is not well known, but elastic fibres of the dermis seem to be the major target of smoke components.
  
  
  OBJECTIVE
  To determine quantitative and qualitative changes of the dermal elastic tissue of non-sun-exposed skin induced by smoking, as well as the possible mechanisms responsible for them.
  
  
  METHODS
  Sixty-nine patients were recruited (20 nonsmokers, 19 former smokers and 30 smokers). Using static morphometry and immunohistochemistry and lectin staining we analysed elastic fibres of the dermis and their major components, elastin and microfibrillar component.
  
  
  RESULTS
  Significantly higher values for the number of elastic fibres mm(-2) and the percentage of the area filled by them in the reticular dermis were found in smokers. Cumulative tobacco dose showed statistically significant correlations with both morphological parameters (Spearman's rank correlation coefficient). Immunohistochemistry demonstrated that the two main components of elastic fibres were altered in smokers. Plasma protease inhibitors and lectin staining were negative in all the samples.
  
  
  CONCLUSIONS
  Smoking is an independent risk factor for the increase of elastic fibres in the reticular dermis of nonexposed skin, and it acts on their two main structural components, elastin and microfibrillar component. This increase in the area of elastic fibres in smokers is not due to newly synthesized elastic material, but to their degradation, as occurs in solar elastosis and which acts in an additive manner.
authors.affiliation: Department of Dermatology, Hospital Figueres, Figueres, Girona, Spain. mjust@wanadoo.es
authors.forename: M
authors.initials: M
authors.lastname: Just
authors.forename: M
authors.initials: M
authors.lastname: Ribera
authors.forename: E
authors.initials: E
authors.lastname: Monsó
authors.forename: J C
authors.initials: JC
authors.lastname: Lorenzo
authors.forename: C
authors.initials: C
authors.lastname: Ferrándiz
mesh_terms: D000328:Adult; D000368:Aged; D004547:Elastic Tissue / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008875:Middle Aged; D015595:Skin Aging / Q000473:pathology*; D012907:Smoking / Q000009:adverse effects / Q000473:pathology*; D044465:White People / Q000208:ethnology