title: A comparison between cultivated and conventional limbal stem cell transplantation for Stevens-Johnson syndrome.
abstract: OBJECTIVE
  To compare the resolution of inflammation and long-term results of cultivated and conventional limbal stem cell transplantation (LSCT) in a patient with Stevens-Johnson syndrome (SJS).
  
  
  METHODS
  Interventional case report.
  
  
  METHODS
  A 32-year-old man with SJS and bilateral total limbal stem cell deficiency underwent cultivated LSCT in the right eye, followed by conventional LSCT in the left eye three weeks later. The postoperative medication included dexamethasone 0.1% and ofloxacin 0.3% eyedrops and a tapering dose of systemic corticosteroid, cyclosporine, and cyclophosphamide. Tear samples were collected and analyzed for interleukin (IL) 8 levels.
  
  
  RESULTS
  Complete corneal epithelialization was achieved 48 hours after cultivated LSCT, compared with three weeks after conventional LSCT. Ocular inflammation and IL-8 levels decreased more rapidly in the eye with cultivated LSCT. Four years after surgery, more severe corneal scarring and opacification were noted in the conventional LSCT eye.
  
  
  CONCLUSIONS
  Cultivated LSCT resulted in a better clinical result and vision, with less stromal scarring compared with conventional LSCT.
authors.affiliation: Department of Ophthalmology, Kyoto Prefectural University of Medicine, 465 Kaiji-cho, Hirokoji-agani, Kawaramachi-dori, Kamigyo-ku, Kyoto 602-0841, Japan.
authors.forename: Leonard P K
authors.initials: LP
authors.lastname: Ang
authors.forename: Chie
authors.initials: C
authors.lastname: Sotozono
authors.forename: Noriko
authors.initials: N
authors.lastname: Koizumi
authors.forename: Tomo
authors.initials: T
authors.lastname: Suzuki
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Inatomi
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kinoshita
mesh_terms: D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology*; D002478:Cells, Cultured; D003316:Corneal Diseases / Q000378:metabolism / Q000601:surgery*; D004847:Epithelial Cells / Q000637:transplantation; D019573:Epithelium, Corneal / Q000166:cytology*; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D016850:Limbus Corneae / Q000166:cytology*; D008297:Male; D033581:Stem Cell Transplantation; D013262:Stevens-Johnson Syndrome / Q000378:metabolism / Q000601:surgery*; D013666:Tears / Q000378:metabolism
chemical_list: D016209:Interleukin-8