title: Identification of a transposon-like insertion in a Glu-1 allele of wheat.
abstract: The Glu-1 locus, present on the long arms of the group 1 chromosomes of wheat, codes for a group of storage protein polypeptides termed high molecular weight (HMW) subunits of glutenin. Hexaploid wheat varieties carry a 'silent' Glu-1y allele on chromosome 1A, no polypeptide being attributable to this locus. When two such alleles from different varieties were compared, one was found to contain an 8 kb insertion of DNA, termed Wis-2, interrupting the coding sequence. The insertion site is flanked by a 5 bp duplication. The two ends of Wis-2 contain similar sequences over 500 bp long and its termini contain almost the same short sequences but in opposite orientation. These terminal sequences are related to those of several 'retroposon'-type transposable elements found in other organisms.
authors.affiliation: Molecular Genetics Department, Plant Breeding Institute, Maris Lane, Trumpington, Cambridge, CB2 2LQ, UK.
authors.forename: N P
authors.initials: NP
authors.lastname: Harberd
authors.forename: R B
authors.initials: RB
authors.lastname: Flavell
authors.forename: R D
authors.initials: RD
authors.lastname: Thompson
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics; D005983:Glutens / Q000235:genetics; D008969:Molecular Sequence Data; D014908:Triticum / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D005983:Glutens; C037302:glutenin