title: Molecular diversity of microbial community in acid mine drainages of Yunfu sulfide mine.
abstract: Two acid mine drainage (AMD) samples were studied by a PCR-based cloning approach, which were from Yunfu sulfide mine in Guangdong province, China. A total of 15 operational taxonomic units (OTUs) were obtained from the two AMD samples. The percentage of overlapped OTUs in two AMD samples was 42.1%. Phylogenetic analysis revealed that the bacterium in the two samples fell into four putative divisions, which were Nitrospira, alpha-Proteobacteria, beta-Proteobacteria, and gamma-Proteobacteria four families. Organisms of genuses Acidithiobacillus and Gallionella, which were in gamma-Proteobacteria family and beta-Proteobacteria family, respectively, were dominant in two samples. The proportions of clones affiliated with Gallionella in each sample were 47.2% (G2) and 16.9% (G1). The result suggested that organisms of Gallionella were a very important composition in microbial communities of the two AMD samples we studied. In addition, the PCR amplification of archaeal 16S rDNA genes form these two AMD samples have been performed with two sets of archaea-specific primers, but no PCR product found.
authors.affiliation: School of Resources Processing and Bioengineering, Central South University, Changsha, 410083, China.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: He
authors.forename: Shengmu
authors.initials: S
authors.lastname: Xiao
authors.forename: Xuehui
authors.initials: X
authors.lastname: Xie
authors.forename: Hui
authors.initials: H
authors.lastname: Zhong
authors.forename: Yuehua
authors.initials: Y
authors.lastname: Hu
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Li
authors.forename: Fenglin
authors.initials: F
authors.lastname: Gao
authors.forename: Guiyuan
authors.initials: G
authors.lastname: Li
authors.forename: Jianshe
authors.initials: J
authors.lastname: Liu
authors.forename: Guanzhou
authors.initials: G
authors.lastname: Qiu
mesh_terms: D000143:Acids; D001419:Bacteria / Q000235:genetics*; D044822:Biodiversity; D002681:China; D008906:Mining; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D014871:Water Microbiology; D014874:Water Pollutants, Chemical
chemical_list: D000143:Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical