Syntheses of conjugated pyrones for the enzymatic assay of lovastatin nonaketide synthase, an iterative polyketide synthase.
[reaction: see text] Lovastatin nonaketide synthase (LovB, LNKS) is an iterative type I polyketide synthase (PKS) from Aspergillus terreus that produces dihydromonacolin L (2), the biosynthetic precursor of lovastatin (1), from acetyl CoA, malonyl CoA, and S-adenosylmethionine (SAM) if the accessory protein LovC is present. In the absence of LovC, LNKS forms the conjugated pyrones 3 and 4 as truncated PKS products. Short syntheses of these pyrones provide material for the assay of LNKS activity by HPLC and radioisotope dilution analysis.
Burr
Douglas A
DA
Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2G2.
Chen
Xuan B
XB
Vederas
John C
JC
D008148:Lovastatin / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D009281:Naphthalenes / Q000737:chemistry / Q000378:metabolism; D048630:Polyketide Synthases / Q000378:metabolism*; D011753:Pyrones / Q000138:chemical synthesis / Q000737:chemistry*
D009281:Naphthalenes; D011753:Pyrones; D048630:Polyketide Synthases; C045373:dihydromonacolin L; D008148:Lovastatin