title: Quercetin, Siamois 1 and Siamois 2 induce apoptosis in human breast cancer MDA-mB-435 cells xenograft in vivo.
abstract: We sought to investigate the apoptosis-inducing activities of quercetin, Siamois 1, and Siamois 2 against invasive estrogen-receptor negative MDA-MB 435 cells xenografted in athymic nude mice. This study clearly demonstrated that these compounds exhibited apoptosis-inducing activities in cell culture system. Quercetin (20 microg/mL), Siamois 1 (100 microg/mL), and Siamois 2 (200 microg/mL) can induce apoptotic cell death by 40 +/-5%, 44 +/- 14 %, and 31 +/- 13 %, respectively. Two-fold of IC50 of these compounds were clearly found to induce apoptosis in breast tumor tissue which can be determined by 99mTc-Annexin V scintigraphy and histological staining. This is the first report that the apoptosis-inducing effects of quercetin, Siamois 1 and Siamois 2 on the MDA-MB 435 cell in vitro were effectively extrapolated to the in vivo situation. These compounds might be considered as a simple dietary supplement and with further clinical investigation for their use as a nutrition-based intervention in breast cancer treatment.
authors.affiliation: Laboratory of Physical Chemistry, Molecular and Cellular Biology, Center of Excellence for Molecular Imaging, Department of Radiologic Technology, Faculty of Associated Medical Sciences, Chiong Mai University, Chiong Mai, Thailand.
authors.forename: Samarn
authors.initials: S
authors.lastname: Dechsupa
authors.forename: Suchart
authors.initials: S
authors.lastname: Kothan
authors.forename: Jackie
authors.initials: J
authors.lastname: Vergote
authors.forename: Gerard
authors.initials: G
authors.lastname: Leger
authors.forename: Antoine
authors.initials: A
authors.lastname: Martineau
authors.forename: Simone
authors.initials: S
authors.lastname: Berangeo
authors.forename: Rachain
authors.initials: R
authors.lastname: Kosanlavit
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Moretti
authors.forename: Samlee
authors.initials: S
authors.lastname: Mankhetkorn
mesh_terms: D000818:Animals; D017209:Apoptosis; D001943:Breast Neoplasms / Q000473:pathology*; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50; D051379:Mice; D008819:Mice, Nude; D011794:Quercetin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D023041:Xenograft Model Antitumor Assays
chemical_list: C521759:Siamois 1; C521760:Siamois 2; D011794:Quercetin