Determination of clarithromycin in human plasma by liquid chromatography-electrospray ionization tandem mass spectrometry.
A rapid and sensitive method has been developed for the determination of clarithromycin in human plasma with liquid chromatography-tandem mass spectrometry. Clarithromycin and the internal standard, telmisartan were precipitated from the matrix (50 microl) with 200 microl acetonitrile and separated by HPLC using formic acid:10 mM ammonium acetate:methanol (1:99:400, v/v/v) as the mobile phase. The assay based on detection by electrospray positive ionization mass spectrometry in the multiple-reaction monitoring mode was finished within 2.4 min. Linearity was over the concentration range 10-5000 ng/ml with a limit of detection of 0.50 ng/ml. Intra- and inter-day precision measured as relative standard deviation were <3.73% and <9.93%, respectively. The method was applied in a bioequivalence study of two tablet formulations of clarithromycin.
Jiang
Yao
Y
Research Center for Drug Metabolism, College of Life Science, Jilin University, Changchun 130023, China.
Wang
Jiang
J
Li
Hao
H
Wang
Yingwu
Y
Gu
Jingkai
J
D000900:Anti-Bacterial Agents / Q000097:blood* / Q000737:chemistry; D001562:Benzimidazoles / Q000737:chemistry; D001565:Benzoates / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D017291:Clarithromycin / Q000097:blood* / Q000737:chemistry; D006801:Humans; D015394:Molecular Structure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000077333:Telmisartan; D013997:Time Factors
D000900:Anti-Bacterial Agents; D001562:Benzimidazoles; D001565:Benzoates; D017291:Clarithromycin; D000077333:Telmisartan