title: Use of repeating dispensers to increase the efficiency of the intramuscular myogenic cell injection procedure.
abstract: Intramuscular myoblast transplantation in humans and nonhuman primates requires precise repetitive cell injections very close to each other. Performed with syringes operated manually throughout large regions, this procedure takes a lot of time, becoming tiring and thus imprecise. We tested two repetitive dispensers with Hamilton syringes as cell injection devices to facilitate this procedure. Monkeys received intramuscular allotransplantations of beta-galactosidase-labeled myoblasts, using either a monosyringe or a multisyringe repeating dispenser. The monosyringe repeating dispenser allowed performing cell injections faster and easier than with a manually operated syringe. The multisyringe dispenser accelerated the procedure still more, but it was not ergonomic. Biopsies of the myoblast-injected sites 1 month later showed abundant beta-galactosidase-positive myofibers, with the same density and morphological pattern observed following myoblast transplantation with a syringe operated manually. We recommend the monosyringe repeating dispenser for myoblast transplantation in skeletal muscles and maybe in the heart.
authors.affiliation: Unité de recherche en Génétique humaine, Centre Hospitalier de l'Université Laval, Québec, QC, Canada. Daniel.Skuk@anm.ulaval.ca
authors.forename: Daniel
authors.initials: D
authors.lastname: Skuk
authors.forename: Marlyne
authors.initials: M
authors.lastname: Goulet
authors.forename: Jacques P
authors.initials: JP
authors.lastname: Tremblay
mesh_terms: D000818:Animals; D001331:Automation; D017690:Cell Transplantation / Q000295:instrumentation* / Q000379:methods; D007273:Injections, Intramuscular / Q000295:instrumentation* / Q000379:methods; D008252:Macaca fascicularis; D024510:Muscle Development / Q000502:physiology; D018482:Muscle, Skeletal / Q000166:cytology* / Q000502:physiology; D032448:Myoblasts, Skeletal / Q000166:cytology / Q000637:transplantation*; D009203:Myocardial Infarction / Q000473:pathology / Q000601:surgery; D013594:Syringes; D014184:Transplantation, Homologous / Q000295:instrumentation / Q000379:methods