title: RAPD with microsatellite as a tool for differentiation of Candida genus yeasts isolated in brewing.
abstract: Fifteen wild yeast strains were isolated in two factories of a lager brewing company in Poland. Their identification with API 32C system showed mainly the presence of Candida sake species (7/15). To differentiate the isolates, randomly amplified polymorphic DNA (RAPD) with (GTG)(5), (GAC)(5), (GACA)(4) microsatellite primers and M13 core sequence (5'-GAG GGT GGC GGT TCT-3') were chosen. The results of patterns similarity are presented as dendrograms for each RAPD analysis and for overall patterns. On the overall patterns, all isolates identified as C. sake, except Strain No. 1, were regrouped in one cluster. Collection strain C. sake CBS 617 was similar in 46% to the cluster with six isolates (Strain Nos. 3, 6, 8, 11, 13, 14). The second reference strain C. sake CBS 159 and the Strain No. 1 were regrouped with other Candida species (collection strains) showing, respectively, only 20% and 42% of similarity to other C. sake strains. The similarity based on the overall dendrogram between isolate Nos. 3, 6, 8, 11, 13, 14 and C. sake CBS 617 was 49%. Between those strains and other Candida, the similarity was only 37%.
authors.affiliation: Department of Biotechnology and Food Microbiology, Faculty of Food Science Agricultural University of Wrocław, Norwida 25, 50-375 Wrocław, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Walczak
authors.forename: Anna
authors.initials: A
authors.lastname: Czaplińska
authors.forename: Wojciech
authors.initials: W
authors.lastname: Barszczewski
authors.forename: Maciej
authors.initials: M
authors.lastname: Wilgosz
authors.forename: Maria
authors.initials: M
authors.lastname: Wojtatowicz
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Robak
mesh_terms: D001515:Beer / Q000382:microbiology*; D002175:Candida / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000032:analysis*; D005516:Food Microbiology; D018895:Microsatellite Repeats; D010802:Phylogeny; D011044:Poland; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D013045:Species Specificity
chemical_list: D004271:DNA, Fungal