Application of PCR-DGGE in research of bacterial diversity in drinking water.
OBJECTIVE
To analyze the structure of bacteria in drinking water by molecular biological techniques.


METHODS
DNA of bacteria in drinking water was directly extracted without culture. 16S ribosomal DNA fragments, including V-6, -7, and -8 regions, were amplified with universal primers (EUBf933GC and EUBr1387) and analyzed by DGGE.


RESULTS
DGGE indicated that amplification products could be separated. The results showed that DGGE could be used in the separation of different microbial 16SrRNA genes extracted from drinking water. Though there were special bacteria in different water samples, the predominant bacteria were essentially the same. Three sequences of the reclaimed specific bands were obtained, and phylogenetic tree of these bands was made.


CONCLUSIONS
Bacterial diversity in drinking water is identified by molecular biological techniques.
Wu
Qing
Q
Department of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China. wuq_molgen@sohu.com
Zhao
Xin-Hua
XH
Zhao
Sheng-Yue
SY
D001419:Bacteria / Q000201:enzymology; D044822:Biodiversity; D004587:Electrophoresis, Agar Gel / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D014871:Water Microbiology
D012336:RNA, Ribosomal, 16S