A multielectrode microcompartment culture platform for studying signal transduction in the nervous system.
This paper describes the design, fabrication, and characterization of a microfabricated compartmented culture system (micro-CCS) useful for electrophysiological signaling studies in cultured neurons. The focus of the paper is the process of interfacing the micro-CCS with cultured neurons and to demonstrate the applicability of the system for biochemical-mediated electrophysiological studies. Moreover, we show that we can record action potentials from cultured neurons through the extracellular compartmented application of elevated levels of K(+) ions. Finally, we show that we can isolate the electrophysiological effects of the sodium channel blocker tetrodotoxin in one of the compartments of a two compartment culture while recording electrophysiological data from both compartments.
Ravula
Surendra K
SK
Georgia Institute of Technology, Microelectronics Research Building, Atlanta, GA 30332, USA.
McClain
Maxine A
MA
Wang
Min S
MS
Glass
Jonathan D
JD
Frazier
A Bruno
AB
D000818:Animals; D002478:Cells, Cultured; D004594:Electrophysiology; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D008839:Microelectrodes; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods*; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D011188:Potassium / Q000502:physiology; D011189:Potassium Chloride / Q000494:pharmacology; D011247:Pregnancy; D011270:Pregnancy, Animal; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D026941:Sodium Channel Blockers / Q000494:pharmacology; D013499:Surface Properties; D013779:Tetrodotoxin / Q000494:pharmacology
D026941:Sodium Channel Blockers; D013779:Tetrodotoxin; D011189:Potassium Chloride; D011188:Potassium