Exogenous IL-1beta induces its own expression, but not that of IL-6 in the hypothalamus and activates HPA axis and prolactin release.
OBJECTIVE
Proinflammatory cytokines IL-1beta, and IL-6 are synthesized in the brain, where they exert local regulatory functions. Our aim was to find out whether, along with the activation of hypothalamo-pituitary-adrenocortical (HPA) axis and prolactin (PRL), the acute systemic enhancement of IL-1beta affects its own production in the hypothalamus as well as that of IL-6.


METHODS
Forty five minutes after a single i.p. administration of recombinant rat IL-1beta (5 microg/kg) to male Long Evans rats we estimated the expression of IL-1beta and IL-6 mRNA in the hypothalamus by real time PCR, ACTH, corticosterone (CORT), and PRL by RIA RESULTS: IL-1beta administration stimulated the expression of IL-1beta mRNA in the hypothalamus by 99 %, but not that of IL-6. It also significantly activated plasma levels of ACTH, PRL, CORT, and CORT production in adrenal gland.


CONCLUSIONS
These results indicate that acute peripheral enhancement of IL-1beta may induce neuroendocrine changes also via the immediate activation of its own expression in the hypothalamus, but not that of IL-6 expression in the hypothalamus was found.
Skurlova
Martina
M
Institue of Normal, Clinical and Pathological Physiology, Third Medical Faculty, Charles University, 120 00 Prague, Czech Republic.
Stofkova
Andrea
A
Jurcovicova
Jana
J
D000324:Adrenocorticotropic Hormone / Q000097:blood; D000818:Animals; D003345:Corticosterone / Q000097:blood; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects*; D007031:Hypothalamus / Q000187:drug effects* / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism* / Q000494:pharmacology*; D015850:Interleukin-6 / Q000378:metabolism*; D008297:Male; D010913:Pituitary-Adrenal System / Q000187:drug effects* / Q000378:metabolism; D011388:Prolactin / Q000097:blood / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D020318:Rats, Long-Evans
D053583:Interleukin-1beta; D015850:Interleukin-6; D012333:RNA, Messenger; D000324:Adrenocorticotropic Hormone; D011388:Prolactin; D003345:Corticosterone