Regulatory effect of amino acids on the pasting behavior of potato starch is attributable to its binding to the starch chain.
The binding of an amino acid, glycine (Gly), alanine (Ala), epsilon-aminocaproic acid (-AC), monosodium glutamate (GluNa), or lysine (Lys), to starch was examined by a biomolecular interaction analyzer (IAsys). A starch sample (ATS) hydrolyzed to an extent of 1% hydrolysis rate with 15% sulfuric acid was used as a model starch for the binding examination. The reducing end of ATS was oxidized by the Somogyi reagent, and the conversion of the reducing end to the carboxyl group of ATS was confirmed by a carboxylic acid fluorescence labeling reagent. The oxidized ATS was immobilized to the amino group of a sensor cuvette by using water-soluble carbodiimide and N-hydroxysuccinimide through an amide bond. The IAsys examination showed that Gly, Ala, and epsilon-AC scarcely bound to the immobilized starch chains but that GluNa and Lys favorably bound with their increasing concentrations. The relative binding index (RBI) of each amino acid was defined by the ratio of the slope of the linear regression equation between the binding response and the concentration for each amino acid to that for Gly. Because the relationships between the RBI and the pasting characteristics (pasting temperature, peak viscosity, breakdown, and swelling index) could each be expressed by a linear regression equation with a high correlation coefficient, it is concluded that the regulation of the pasting behavior of starch with an amino acid is caused by binding of the amino acid to the starch chains.
Ito
Azusa
A
Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan,.
Hattori
Makoto
M
Yoshida
Tadashi
T
Watanabe
Ayako
A
Sato
Ryoichi
R
Takahashi
Koji
K
D000596:Amino Acids / Q000378:metabolism*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005998:Glycine / Q000378:metabolism; D006868:Hydrolysis; D010084:Oxidation-Reduction; D011198:Solanum tuberosum / Q000737:chemistry*; D013213:Starch / Q000737:chemistry* / Q000378:metabolism*; D013464:Sulfuric Acids
D000596:Amino Acids; D013464:Sulfuric Acids; D013213:Starch; C033158:sulfuric acid; D005998:Glycine