An electrochemical approach to monitor pH change in agar media during plant tissue culture.
In this work, metal oxide microelectrodes were developed to monitor pH change in agar media during plant tissue culture. An antimony wire was produced by a new approach "capillary melt method". The surface of the obtained antimony wire was oxidized in a potassium nitrate melt to fabricate an antimony oxide film for pH sensing. Characterization results show that the oxide layer grown on the wire surface consists of Sb(2)O(3) crystal phase. The sensing response, open-circuit potential, of the electrode has a good linear relationship (R(2)=1.00) with pH value of the test solution. Adding organic compounds into the test media would not affect the linear relationship, although the slope of the lines varied with different ingredients added. The antimony oxide electrodes were employed to continuously monitor pH change of agar culture media during a 2-week plant tissue culture of Dendrobium candidum. The antimony oxide electrode fabricated this way has the advantages of low cost, easy fabrication, fast response, and almost no contamination introduced into the system. It would be suitable for in situ and continuous pH measurement in many bio applications.
Wang
Min
M
Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310058, China. minwang@zju.edu.cn <minwang@zju.edu.cn>
Ha
Yang
Y
D000362:Agar / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media / Q000737:chemistry*; D031667:Dendrobium / Q000254:growth & development*; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006863:Hydrogen-Ion Concentration; D017736:Ion-Selective Electrodes; D010944:Plants; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D046509:Tissue Culture Techniques / Q000295:instrumentation* / Q000379:methods
D003470:Culture Media; D000362:Agar