Proliferation of activated CD1d-restricted NKT cells is down-modulated by lymphocyte activation gene-3 signaling via cell cycle arrest in S phase.
Upon antigenic stimulation, CD1d-restricted NKT cells quickly secrete large amounts of cytokines. This prompt response demonstrates that CD1d-restricted NKT cells may potentially prove to be useful therapeutic agents for the treatment of many diseases. Despite the clinical importance of CD1d-restricted NKT cells, the regulating mechanisms of this unique T cell population remain to be defined. We found murine LAG-3 is inducible on CD1d-restricted NKT cells as the result of a variety of stimulants such as concanavalin A (con A) and anti-CD3. Also, antigen-specific CD1d stimulation can elicit LAG-3 in CD1d-restricted NKT cells. Moreover, ectopic LAG-3 expression on CD1d-restricted NKT cells results in cell cycle arrest in the S phase. These results show that LAG-3 signaling on activated CD1d-restricted NKT cells may down-modulate NKT cell proliferation.
Byun
Hyun-Jung
HJ
Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Sung-Buk Ku, Anam-Dong, Seoul 136-701, South Korea.
Jung
Woon-Won
WW
Lee
Dong-Sup
DS
Kim
Sanghee
S
Kim
Sang Joon
SJ
Park
Chung-Gyu
CG
Chung
Hee Yong
HY
Chun
Taehoon
T
D000818:Animals; D015703:Antigens, CD / Q000276:immunology*; D018949:Antigens, CD1 / Q000276:immunology*; D049109:Cell Proliferation; D015536:Down-Regulation; D005786:Gene Expression Regulation / Q000276:immunology*; D007155:Immunologic Factors; D007694:Killer Cells, Natural / Q000166:cytology* / Q000276:immunology*; D008213:Lymphocyte Activation / Q000235:genetics*; D051379:Mice; D016196:S Phase / Q000235:genetics; D015398:Signal Transduction / Q000276:immunology*; D014161:Transduction, Genetic; D000093322:Lymphocyte Activation Gene 3 Protein
D015703:Antigens, CD; D018949:Antigens, CD1; D007155:Immunologic Factors; D000093322:Lymphocyte Activation Gene 3 Protein; C000720327:Lag3 protein, mouse