title: Identification of genes from Aspergillus oryzae that are preferentially expressed in membrane-surface liquid culture.
abstract: We identified 22 genes from Aspergillus oryzae that are preferentially expressed in membrane-surface liquid culture (MSLC), among which Ser/Thr protein kinase (aopk1) and phosphatase (aoppt) genes were cloned. We also revealed that aopk1 encodes a protein with an N-terminal sequence 150 amino acid residues longer than that predicted from the registered sequence in GenBank.
authors.affiliation: Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, 3-1-1 Tsushima-naka, Okayama 700-8530, Japan.
authors.forename: Bin
authors.initials: B
authors.lastname: Feng
authors.forename: Masakazu
authors.initials: M
authors.lastname: Morita
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Imanaka
authors.forename: Koreyoshi
authors.initials: K
authors.lastname: Imamura
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Nakanishi
mesh_terms: D000595:Amino Acid Sequence; D001236:Aspergillus oryzae / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017346:Protein Serine-Threonine Kinases; D010744:Phosphoric Monoester Hydrolases