Assessment of adenovirus infection in adult lung transplant recipients using molecular surveillance.
BACKGROUND
Little is known about adenovirus infections in adult lung transplant recipients. Because the virus can establish latency, re-activation may be relatively common after transplantation.


METHODS
We assessed adenovirus infection in 80 adult lung transplant recipients. Adenovirus polymerase chain reaction (real-time PCR assay; limit of detection approximately 25 copies/ml plasma) was done on plasma samples collected at regular intervals until 1 year post-transplant.


RESULTS
Adenovirus DNA was detected in 18 of 80 patients (22.5%) and in 19 of 595 (3.4%) plasma samples up to 12 months post-transplant. Median time to detection of viremia was 134 days post-transplant (range 1 to 370 days). Median viral load was 180 copies/ml plasma (range 50 to 360 copies/ml). Symptoms were evaluated at the time of adenovirus detection: 14 of 18 (78%) patients were asymptomatic; 4 of 18 (22%) patients had otherwise unexplained febrile/flu-like illness that resolved spontaneously. Adenovirus was not found to be a trigger for acute rejection. No detrimental effect on pulmonary function was seen immediately after adenovirus infection.


CONCLUSIONS
Adenovirus viremia is common in adult lung transplant recipients. In contrast to findings on adenoviral pneumonitis in lung transplant recipients, isolated episodes of low-level viremia are self-limited and do not trigger acute rejection or a decline in pulmonary function.
Humar
Atul
A
Department of Medicine, University of Toronto, Toronto, Ontario, Canada. atul.humar@uhn.on.ca
Doucette
Karen
K
Kumar
Deepali
D
Pang
Xiao-Li
XL
Lien
Dale
D
Jackson
Kathy
K
Preiksaitis
Jutta
J
D000256:Adenoviridae / Q000235:genetics*; D000257:Adenoviridae Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000209:etiology* / Q000821:virology; D000328:Adult; D000368:Aged; D004279:DNA, Viral / Q000097:blood*; D005260:Female; D006801:Humans; D015994:Incidence; D016040:Lung Transplantation / Q000009:adverse effects*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011159:Population Surveillance / Q000379:methods*; D011184:Postoperative Period; D012075:Remission, Spontaneous; D013997:Time Factors; D019562:Viral Load; D014766:Viremia / Q000175:diagnosis / Q000453:epidemiology / Q000209:etiology / Q000821:virology
D004279:DNA, Viral