Proteomic analysis of proteins differentially expressed in preeclamptic trophoblasts.
OBJECTIVE
To identify differential trophoblastic proteins associated with preeclampsia (PE) by proteomic analysis.


METHODS
We isolated and purified placental trophoblasts from normotensive pregnant women and patients with PE by a continuous Percoll gradient. The expression of proteins was determined by sliver staining after two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Proteins of interest were identified using matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS).


RESULTS
The overall trophoblastic protein expression patterns in preeclamptic and corresponding normotensive placentas were quite similar except for some areas. Of 34 differentially expressed protein spots (p < 0.05 by paired t-test), seven differential proteins from nine spots were identified by MALDI-TOF-MS. The expression of the following proteins was repressed (p < 0.01): disulfide isomerase ER-60, peroxiredoxin 2, and Delta3,5-Delta2,4-dienoyl-CoA isomerase. Four proteins (protein disulfide isomerase precursor, endoplasmic reticulum resident protein, dihydrolipoyl dehydrogenase and TIM21-like protein) were found to be significantly upregulated in PE (p < 0.01).


CONCLUSIONS
We identified several proteins with significant altered expression in PE using 2D-PAGE. This method is a powerful technique with which to search for not only quantitative but also qualitative changes in a biological process of interest.
Sun
Li-zhou
LZ
Department of Gynecology and Obstetrics, First Affiliated Hospital of Nanjing Medical University, Nanjing Medical University, Nanjing, PR China. lizhou_sun121@hotmail.com
Yang
Na-na
NN
De
Wei
W
Xiao
Yun-shan
YS
D000328:Adult; D015415:Biomarkers / Q000032:analysis; D016022:Case-Control Studies; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D010449:Peptide Mapping; D011225:Pre-Eclampsia / Q000235:genetics / Q000473:pathology*; D011247:Pregnancy; D011336:Probability; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D012016:Reference Values; D012307:Risk Factors; D012494:Sampling Studies; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D014327:Trophoblasts / Q000166:cytology* / Q000502:physiology
D015415:Biomarkers; D011506:Proteins