Nuclear remodeling and nuclear reprogramming for making transgenic pigs by nuclear transfer.
A better understanding of the cellular and molecular events that occur when a nucleus is transferred to the cytoplasm of an oocyte will permit the development of improved procedures for performing nuclear transfer and cloning. In some cases it appears that the gene(s) are reprogrammed, while in other cases there appears to be little effect on gene expression. Not only does the pattern of gene expression need to be reprogrammed, but other structures within the nucleus also need to be remodeled. While nuclear transfer works and transgenic and knockout animals can be created, it still is an inefficient process. However, even with the current low efficiencies this technique has proved very valuable for the production of animals that might be useful for tissue or organ transplantation to humans.
Prather
Randall S
RS
Division of Animal Science, Food for the 21st Century, College of Food, Agriculture & Natural Resources, University of Missouri-Columbia, 920 East Campus Drive, E125 ASRC, Columbia, Missouri 65211-5300, USA. PratherR@missouri.edu
D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D049628:Body Size / Q000235:genetics; D002467:Cell Nucleus / Q000235:genetics*; D042002:Chromatin Assembly and Disassembly / Q000235:genetics*; D044127:Epigenesis, Genetic / Q000235:genetics; D005786:Gene Expression Regulation / Q000235:genetics; D053652:Nuclear Transfer Techniques / Q000592:standards / Q000639:trends*; D034421:Sus scrofa / Q000235:genetics*; D019076:Transgenes / Q000235:genetics