Comparison of the medium molecular weight venom fractions from five species of common social wasps by MALDI-TOF spectra profiling.
The average spectral profiles and the exact mass weight (MW) of biomolecules present in the medium fraction (from 900 to 3000 Da) of the venom of five social wasps (three European and one North American Polistes and the European hornet Vespa crabro) were determined by matrix assisted laser desorption ionization time of flight (MALDI-TOF) MS. Data were obtained analyzing the venom of single specimens (N = 46) and elaborated with the ClinProTools 2.0 (CPT) software to search for differences among the five species examined. Interesting differences in the spectral profiles were found, allowing the discrimination of venoms belonging to the different species, and their possible use as a quality control method in venom immunotherapy (VIT) for allergic patients.
Turillazzi
Stefano
S
Centro Interdipartimentale di Spettrometria di Massa, Università degli Studi di Firenze, Italy. turillazzi@dbag.unifi.it
Bruschini
Claudia
C
Lambardi
Duccio
D
Francese
Simona
S
Spadolini
Igino
I
Mastrobuoni
Guido
G
D000818:Animals; D016002:Discriminant Analysis; D008970:Molecular Weight; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014862:Wasp Venoms / Q000737:chemistry*; D014863:Wasps / Q000502:physiology*
D014862:Wasp Venoms