title: Clinical, histopathological and immunophenotypical findings in five horses with cutaneous malignant lymphoma.
abstract: This study documents the clinical, histopathological, immunohistochemical and flow-cytometric findings in five horses with cutaneous non-epidermotropic malignant lymphoma (ML). The median survival time after discovery of the first subcutaneous nodules was 3.8 years (range 2-5 years: n=4). Histologically, the cutaneous ML had a pleiomorphic structure and contained a mixture of large reticulo-endothelial cells, medium-large sized lymphoid cells with a rounded nucleus and small nucleoli, many medium sized lymphoid cells with irregular nuclei, and some small lymphoid cells. Immunohistochemically (IHC) the lymphoid cells were positive for the pan-T-lymphocyte marker CD3 but negative for the B-lymphocyte markers CD21 and kappa and lambda immunoglobulin light chains. Although routine haematological examination revealed no abnormalities in the horses with cutaneous ML, changes in the peripheral blood lymphocyte population were apparent flow-cytometrically. Compared to clinically healthy horses, a decreased total percentage of cells was recorded in the lymphocyte gate. In three horses with cutaneous ML, an increase in CD4 positive cells was noticed in the monocyte gate. Flow-cytometric analysis of tumour cells collected by fine needle aspiration (FNA) suggested that the cutaneous MLs consisted primarily of CD4 and CD8 positive T-lymphocytes. The results were compared to those of a monomorphic multicentric T- and a monomorphic multicentric B-cell lymphoma. The results of immunohistochemistry and flow-cytometry were largely but not completely in accordance. In conclusion, the results of this study suggest that cutaneous non-epitheliotropic malignant lymphomas in the horse are of T-cell origin and that, after improvement of its accuracy, flow cytometric analysis of FNAs might become a useful aid to rapid tumour identification.
authors.affiliation: Department of Equine Sciences, Utrecht University, 3508 TD, Utrecht, The Netherlands. paarden@dierenkliniekwolvega.nl
authors.forename: C M
authors.initials: CM
authors.lastname: de Bruijn
authors.forename: J N
authors.initials: JN
authors.lastname: Veenman
authors.forename: V P M G
authors.initials: VP
authors.lastname: Rutten
authors.forename: E
authors.initials: E
authors.lastname: Teske
authors.forename: R A
authors.initials: RA
authors.lastname: van Nieuwstadt
authors.forename: T S G A M
authors.initials: TS
authors.lastname: van den Ingh
mesh_terms: D000818:Animals; D001706:Biopsy / Q000662:veterinary; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D006734:Horse Diseases / Q000276:immunology / Q000473:pathology*; D006736:Horses; D007150:Immunohistochemistry / Q000662:veterinary; D008223:Lymphoma / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D008297:Male; D012878:Skin Neoplasms / Q000473:pathology / Q000662:veterinary*