Early archives of genetically-restricted proviral DNA in the female genital tract after heterosexual transmission of HIV-1.
OBJECTIVE
In order to characterize human immunodeficiency virus type 1 (HIV-1) variants that are transmitted in women via heterosexual intercourse, the env V1-V3 sequences of HIV-1 provirus (DNA) and free virus (RNA) in paired samples of blood and cervicovaginal secretions of untreated chronically and primary infected African women were compared.


RESULTS
Env RNA sequences retrieved from plasma and genital compartments formed a single cluster in primary infection. In contrast, env RNA sequences from these two compartments were distinct in chronically infected women. Analysis of proviral DNA of primary infected women showed that most HIV-1 sequences derived from the genital epithelia form independent clusters from HIV-1 sequences of DNA from peripheral blood mononuclear cells and RNA recovered from plasma and genital secretions. Similarly, the analysis of proviral DNA in the genital compartment of chronically infected women showed the persistence of genetically-restricted cluster of HIV-1.


CONCLUSIONS
These observations indicate that a viral subpopulation is archived as proviral DNA in the female genital tract early in primary infection, and suggest that HIV-1 variants from the male donor are selected in the female mucosal site during male to female transmission of HIV-1.
Chomont
Nicolas
N
Université Paris V, Unité INSERM Internationale d'Immunologie Humaine U743, Equipe Immunité et Biothérapie Muqueuse, Centre de Recherches Biomédicales des Cordeliers, and Laboratoire de Virologie, Hôpital Européen Georges Pompidou, Paris, France.
Hocini
Hakim
H
Grésenguet
Gérard
G
Brochier
Céline
C
Bouhlal
Hicham
H
Andréoletti
Laurent
L
Becquart
Pierre
P
Charpentier
Charlotte
C
de Dieu Longo
Jean
J
Si-Mohamed
Ali
A
Kazatchkine
Michel D
MD
Bélec
Laurent
L
D000208:Acute Disease; D002908:Chronic Disease; D004279:DNA, Viral / Q000302:isolation & purification; D005260:Female; D015752:Genes, env; D014644:Genetic Variation; D005836:Genitalia, Female / Q000821:virology*; D015658:HIV Infections / Q000635:transmission* / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011446:Prospective Studies; D011533:Proviruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis / Q000097:blood
D004279:DNA, Viral; D012367:RNA, Viral