Hyperbaric oxygen therapy attenuates renal ischemia/reperfusion injury in rats.
OBJECTIVE
Renal ischemia/reperfusion (I/R) injury occurs in both native and transplanted kidneys. Hyperbaric oxygen (HBO) has been shown to prevent I/R injury in different tissues. The aim of this study was to evaluate the effect of HBO on renal I/R injury in rats.


METHODS
Sprague-Dawley rats were randomly assigned to one of three groups. The Control group (n = 6) received right nephrectomy. The I/R (n = 6) and I/R+HBO groups (n = 6) received 30 min left renal ischemia followed by 24 h of reperfusion after right nephrectomy. The I/R+HBO group (n = 6) received additional HBO therapy for 60 min at 2.5 absolute atmospheres starting at the initial 15th minute of reperfusion.


RESULTS
In the I/R group, blood urea nitrogen (BUN) and creatinine levels increased significantly compared with the Control and I/R+HBO groups (p < 0.05). BUN and creatinine levels were similar in the Control and I/R+HBO groups. Kidney samples from I/R group rats revealed severe tubular damage and neutrophil infiltration at histopathological examination. The animals treated with HBO showed markedly improved lesions and less neutrophil infiltration compared with the I/R group (p < 0.05).


CONCLUSIONS
HBO exhibited marked protection against I/R injury in this study as measured using BUN and creatinine levels and renal histopathology. However, further studies are needed to clarify the renoprotective effect of HBO on I/R injury.
Solmazgul
Emrullah
E
Department of Internal Medicine, Gulhane Military Medical Academy, Haydarpasa Training Hospital, Kadikoy/Istanbul, Turkey.
Uzun
Gunalp
G
Cermik
Hakan
H
Atasoyu
Enes M
EM
Aydinoz
Secil
S
Yildiz
Senol
S
D058186:Acute Kidney Injury / Q000097:blood / Q000209:etiology / Q000628:therapy*; D000818:Animals; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D006801:Humans; D006931:Hyperbaric Oxygenation / Q000379:methods*; D007668:Kidney / Q000098:blood supply / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000097:blood / Q000150:complications / Q000628:therapy*; D016896:Treatment Outcome
D003404:Creatinine