title: Prolonged viral RNA detection in blood and lymphoid tissues from coxsackievirus B4 E2 orally-inoculated Swiss mice.
abstract: The spreading of viral RNA within Swiss Albino mice orally inoculated with coxsackievirus B4 E2 strain (CVB4 E2) was studied by using RT-PCR and semi-nested-RT-PCR methods. Viral RNA was detected in various organs: pancreas, heart, small intestine, spleen, thymus, and blood at various postinfectious (p.i.) times ranging from 8 hr to 150 days. Our results show that (i) outbred mice can be infected with CVB4 E2 following an oral inoculation, which results in systemic spreading of viral RNA, (ii) CVB4 E2 infection can be associated with a prolonged detection of viral RNA in spleen, thymus and blood, up to 70 days p.i. and further in other organ tissues.
authors.affiliation: Service de Virologie/UPRES EA3610, Faculté de Médecine, Université Lille 2, Bâtiment Paul Boulanger, CHRU Lille, Lille, France.
authors.forename: Hela
authors.initials: H
authors.lastname: Jaïdane
authors.forename: Jawhar
authors.initials: J
authors.lastname: Gharbi
authors.forename: Pierre-Emmanuel
authors.initials: PE
authors.lastname: Lobert
authors.forename: Bernadette
authors.initials: B
authors.lastname: Lucas
authors.forename: Raïda
authors.initials: R
authors.lastname: Hiar
authors.forename: Manel Ben
authors.initials: MB
authors.lastname: M'hadheb
authors.forename: Fabienne
authors.initials: F
authors.lastname: Brilot
authors.forename: Vincent
authors.initials: V
authors.lastname: Geenen
authors.forename: Mahjoub
authors.initials: M
authors.lastname: Aouni
authors.forename: Didier
authors.initials: D
authors.lastname: Hober
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000378:metabolism; D003384:Coxsackievirus Infections / Q000097:blood / Q000821:virology*; D029822:Enterovirus B, Human; D008221:Lymphoid Tissue / Q000473:pathology / Q000821:virology*; D051379:Mice; D012367:RNA, Viral / Q000032:analysis / Q000097:blood*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D012367:RNA, Viral