Collapsin response mediator protein 1 mediates reelin signaling in cortical neuronal migration.
Collapsin response mediator protein 1 (CRMP1) is one of the CRMP family members that mediates signal transduction of axon guidance molecules. Here, we show evidence that CRMP1 is involved in Reelin (Reln) signaling to regulate neuronal migration in the cerebral cortex. In crmp1-/- mice, radial migration of cortical neurons was retarded. This phenotype was not observed in the sema3A-/- and crmp1+/-;sema3A+/- cortices. However, CRMP1 was colocalized with disabled-1 (Dab1), an adaptor protein in Reln signaling. In the Reln(rl/rl) cortex, CRMP1 and Dab1 were expressed at a higher level, yet tyrosine phosphorylated at a lower level. Loss of crmp1 in a dab1 heterozygous background led to the disruption of hippocampal lamination, a Reeler-like phenotype. In addition to axon guidance, CRMP1 regulates neuronal migration by mediating Reln signaling.
Yamashita
Naoya
N
Department of Molecular Pharmacology and Neurobiology, Yokohama City University Graduate School of Medicine, Yokohama 236-0004, Japan.
Uchida
Yutaka
Y
Ohshima
Toshio
T
Hirai
Syu-ichi
S
Nakamura
Fumio
F
Taniguchi
Masahiko
M
Mikoshiba
Katsuhiko
K
Honnorat
Jérôme
J
Kolattukudy
Pappachan
P
Thomasset
Nicole
N
Takei
Kohtaro
K
Takahashi
Takuya
T
Goshima
Yoshio
Y
D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D002465:Cell Movement / Q000235:genetics / Q000502:physiology*; D002540:Cerebral Cortex / Q000166:cytology / Q000502:physiology*; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000502:physiology*; D006801:Humans; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008818:Mice, Neurologic Mutants; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D010750:Phosphoproteins / Q000235:genetics / Q000502:physiology*; D000091042:Reelin Protein; D012697:Serine Endopeptidases / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology*; D000098855:Collapsin Response Mediator Protein 1
D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D000091042:Reelin Protein; D000098855:Collapsin Response Mediator Protein 1; C000717127:RELN protein, human; C000717128:Reln protein, mouse; D012697:Serine Endopeptidases