First analysis of macromolecular crystals: biochemistry and x-ray diffraction.
Methods are described for performing a first analysis of newly prepared macromolecular crystals. Biochemical analysis using denaturing gel electrophoresis on dissolved crystals informs on the content of the crystal. Detailed protocols are given for mounting crystals in capillaries or fiber loops in preparation for analysis of their X-ray diffractive properties.
Jeruzalmi
David
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Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, USA.
D003460:Crystallization / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D046911:Macromolecular Substances / Q000737:chemistry*; D014961:X-Ray Diffraction / Q000379:methods*
D046911:Macromolecular Substances