title: CD312, the human adhesion-GPCR EMR2, is differentially expressed during differentiation, maturation, and activation of myeloid cells.
abstract: EMR2/CD312 is a member of the adhesion-GPCR family that contains extracellular EGF-like domains. Previously it has been shown to interact with chondroitin sulphate glycosaminoglycans in an isoform-specific manner. Although EMR2 expression has been found to be restricted to human myeloid cells, its expression profile has not yet been systemically characterized. In this report, we show that EMR2 receptor expression is up-regulated during differentiation and maturation of macrophages, and is conversely down-regulated during dendritic cell maturation. We also demonstrate that EMR2 receptor alternative splicing and glycosylation is regulated during myeloid differentiation. In monocytes and macrophages, EMR2 can be specifically up-regulated by LPS and IL-10 via an IL-10-mediated pathway. In inflamed tissues, EMR2 is detected in subpopulations of myeloid cells including macrophages and neutrophils. The results presented here further support the idea that EMR2 plays a role in the migration and adhesion of myeloid cells during cell differentiation, maturation, and activation.
authors.affiliation: Sir William Dunn School of Pathology, The University of Oxford, South Parks Road, Oxford, OX1 3RE, UK.
authors.forename: Gin-Wen
authors.initials: GW
authors.lastname: Chang
authors.forename: John Q
authors.initials: JQ
authors.lastname: Davies
authors.forename: Martin
authors.initials: M
authors.lastname: Stacey
authors.forename: Simon
authors.initials: S
authors.lastname: Yona
authors.forename: Dawn M E
authors.initials: DM
authors.lastname: Bowdish
authors.forename: Jörg
authors.initials: J
authors.lastname: Hamann
authors.forename: Tse-Ching
authors.initials: TC
authors.lastname: Chen
authors.forename: Chun-Yen
authors.initials: CY
authors.lastname: Lin
authors.forename: Siamon
authors.initials: S
authors.lastname: Gordon
authors.forename: Hsi-Hsien
authors.initials: HH
authors.lastname: Lin
mesh_terms: D002454:Cell Differentiation / Q000276:immunology; D049108:Cell Enlargement; D002460:Cell Line; D005786:Gene Expression Regulation / Q000276:immunology; D006801:Humans; D022423:Myeloid Cells / Q000166:cytology* / Q000276:immunology*; D018375:Neutrophil Activation / Q000276:immunology*; D043562:Receptors, G-Protein-Coupled / Q000276:immunology*
chemical_list: C412456:ADGRE2 protein, human; D043562:Receptors, G-Protein-Coupled