title: Optimized dyeing conditions of immunoprotein with reactive dye Procion Blue MX-7RX.
abstract: For investigating the feasibility of using reactive dyes as an immunoassay marker, a dichlorine triazine dye, Procion Blue MX-7RX, was employed to stain the antibody against human serum albumin (anti-HSA). With the color intensity revealed in the immunochromatographic test strip as the objective variables, the optimal dyeing conditions were found as follows: pH 11.4, temperature 35.7 degrees C, molar ratio 188 (mol dye/mol antibody), and reaction time 45.6 min. The dyed-anti-HSA revealed a maximal color intensity of 8738 without apparent loss of antigen binding affinity.
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, Taichung 402, Taiwan, ROC.
authors.forename: Shwu-Jinng
authors.initials: SJ
authors.lastname: Wang
authors.forename: Hsiu-Min
authors.initials: HM
authors.lastname: Lin
authors.forename: Xin-Ren
authors.initials: XR
authors.lastname: Wang
authors.forename: Kuang-Pin
authors.initials: KP
authors.lastname: Hsiung
authors.forename: Yung-Chuan
authors.initials: YC
authors.lastname: Liu
mesh_terms: D000906:Antibodies / Q000737:chemistry*; D004396:Coloring Agents / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay; D012709:Serum Albumin / Q000032:analysis* / Q000276:immunology*; D013696:Temperature; D014227:Triazines / Q000737:chemistry*
chemical_list: D000906:Antibodies; D004396:Coloring Agents; D012709:Serum Albumin; D014227:Triazines; C036598:procion blue MX-R