Time-resolved methods in biophysics. 3. Fluorescence lifetime correlation spectroscopy.
We present a thorough introduction into the recently developed fluorescence lifetime correlation spectroscopy (FLCS). The theoretical basis of FLCS is explained, and the method is applied to the study of a dynamic transition between two fluorescence lifetime states in a dye-protein complex.
Gregor
Ingo
I
Institute for Biological Information Processing 1, Forschungszentrum Jülich, D-52425, Jülich, Germany. i.gregor@fz-juelich.de
Enderlein
Jörg
J
D055592:Biophysical Phenomena; D001703:Biophysics; D002232:Carbocyanines / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D008956:Models, Chemical; D008968:Molecular Conformation; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence / Q000379:methods*; D019809:Streptavidin / Q000737:chemistry*; D013997:Time Factors
D002232:Carbocyanines; D005456:Fluorescent Dyes; C085321:cyanine dye 5; D019809:Streptavidin