The transport of alpha(1A)-adrenergic receptor with 33-nm step size in live cells.
We used the technique of single particle tracking (SPT) with high tempo-spatial resolution to efficiently explore the route and mechanism for the transport of alpha(1A)-adrenergic receptor (alpha(1A)-AR) in real time in living cells. We found that the initial transport of alpha(1A)-AR in cells depended on actin filaments with the velocity of 0.2 microm/s and exhibited discrete 33-nm steps. It was noted that the step size, the rate constant, and the velocities were in accordance with the character of single myosin in vitro, implying that while transporting each endosome myosins did not work in the "tug-of-war" mode and that they did not adopt the strategy to boost up transporting speed by working coordinately. These results provided insight into the mechanism of GPCR transport in vivo.
Liang
Zhang-Yi
ZY
State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing, China. zhangyy@bjmu.edu.cn
Xu
Ning
N
Guan
Ying-Hua
YH
Xu
Ming
M
He
Qi-Hua
QH
Han
Qi-De
QD
Zhang
You-Yi
YY
Zhao
Xin-Sheng
XS
D000199:Actins / Q000502:physiology*; D002460:Cell Line; D006801:Humans; D007668:Kidney / Q000502:physiology*; D020409:Molecular Motor Proteins / Q000502:physiology*; D021381:Protein Transport / Q000502:physiology*; D018340:Receptors, Adrenergic, alpha-1 / Q000502:physiology*
C547719:ADRA1D protein, human; D000199:Actins; D020409:Molecular Motor Proteins; D018340:Receptors, Adrenergic, alpha-1