Different effects of high molecular weight sodium hyaluronate and NSAID on the progression of the cartilage degeneration in rabbit OA model.
OBJECTIVE
We examined the effects of nonsteroidal anti-inflammatory drug (NSAID) and intra-articular injections of sodium hyaluronate (HA) on cartilage degeneration in rabbit knee osteoarthritis (OA) model induced by partial meniscectomy.


METHODS
Loxoprofen sodium (LOX) was used as the NSAID, administered p.o. once daily for 14 days at a dose of 4 mg/kg, starting from the day of meniscectomy. Suvenyl(SVE) was used as the high molecular weight HA, injected intra-articularly into injured knee every 3 days from the day of surgery. The effect on pain was assessed by an Incapacitance Tester, and the damage of cartilage was evaluated by visual assessment and histopathology at 14 days after the surgery.


RESULTS
Hind paw weight bearing on the injured paw decreased time-dependently in the control group. In other three groups, the decrease of hind paw weight distribution was suppressed, demonstrating an analgesic effect. Visible damage and histopathological findings of cartilage degeneration were evident in the cartilages of the control group at day 14. In the SVE group, the damaged cartilage area decreased and cartilage degeneration was ameliorated. In contrast, in the LOX group, the cartilage degeneration was augmented compared with the control group. And, this exacerbated cartilage degeneration induced by LOX was reversed by the concomitant use of SVE.


CONCLUSIONS
Our data show that the treatment with high molecular weight HA from earlier stage is strongly recommended to preserve the structure of cartilage.
Mihara
M
M
Product Research Department, Chugai Pharmaceutical Co., Ltd., Shizuoka, Japan. miharamsh@chugai-pharm.co.jp
Higo
S
S
Uchiyama
Y
Y
Tanabe
K
K
Saito
K
K
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000494:pharmacology*; D002358:Cartilage, Articular / Q000187:drug effects* / Q000473:pathology; D004195:Disease Models, Animal; D006614:Hindlimb; D006820:Hyaluronic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D007270:Injections, Intra-Articular; D008297:Male; D010003:Osteoarthritis / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D010146:Pain; D011817:Rabbits; D016474:Weight-Bearing
D000276:Adjuvants, Immunologic; D000894:Anti-Inflammatory Agents, Non-Steroidal; D006820:Hyaluronic Acid