title: Young and middle-age associated differences in cytokeratin expression after bone fracture, tissue trauma, and hemorrhage.
abstract: BACKGROUND
  This study examined whether there is any difference in the lung cytokine and cytokeratin 19 levels between young and middle-aged mice after bone fracture and soft-tissue trauma hemorrhage (Fx-TH).
  
  
  METHODS
  Young (6-8 weeks) and middle-aged (12 months) C3H/HeN male mice were subjected to right lower leg fracture, trauma hemorrhage (mean arterial blood pressure to 35 +/- 5 mm Hg for 90 minutes), and resuscitation.
  
  
  RESULTS
  The tumor necrosis factor alpha level in the lung increased significantly at 2 hours after Fx-TH in both young and middle-aged mice, whereas at 24 hours the levels remained significantly higher in middle-aged mice. Interleukin-6 levels increased significantly 24 hours after Fx-TH in both groups, whereas interleukin-10 levels increased only in middle-aged mice at 24 hours under those conditions. Monocyte chemoattractant protein-1 levels increased significantly 2 hours after Fx-TH. The protein and messenger RNA levels of cytokeratin 19 were significantly higher in middle-aged mice compared with young mice after Fx-TH.
  
  
  CONCLUSIONS
  These results suggest that age influences the lung inflammatory response after Fx-TH.
authors.affiliation: Center for Surgical Research and Department of Surgery, University of Alabama at Birmingham, G 094 Volker Hall, 1670 University Blvd, Birmingham, AL 35294-0019, USA.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Matsutani
authors.forename: Shih-Ching
authors.initials: SC
authors.lastname: Kang
authors.forename: Masao
authors.initials: M
authors.lastname: Miyashita
authors.forename: Koji
authors.initials: K
authors.lastname: Sasajima
authors.forename: Mashkoor A
authors.initials: MA
authors.lastname: Choudhry
authors.forename: Kirby I
authors.initials: KI
authors.lastname: Bland
authors.forename: Irshad H
authors.initials: IH
authors.lastname: Chaudry
mesh_terms: D000367:Age Factors; D000375:Aging / Q000378:metabolism*; D000818:Animals; D016207:Cytokines / Q000378:metabolism*; D050723:Fractures, Bone / Q000378:metabolism*; D006470:Hemorrhage / Q000378:metabolism*; D053539:Keratin-19 / Q000378:metabolism*; D008168:Lung / Q000378:metabolism*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D017695:Soft Tissue Injuries / Q000378:metabolism*
chemical_list: D016207:Cytokines; D053539:Keratin-19