The effect of substance P on nitric oxide production by HSV-1 infected macrophages.
The regulatory role of neuro-immune pathways in various infectious disease states is an interesting area of investigation due to its important therapeutic potential. In this study the effect of substance P (SP) on nitric oxide (NO) production of HSV-infected macrophages and its relation with incubation time and also the cytopathic effect of virus in the presence of SP are considered. As we have observed, substance P enhances NO production both in HSV- and mock-infected macrophages and this effect has been diminished or disappeared after 24 h. It has also been observed that SP enhances the cytopathic effect of HSV in a time dependent manner with the most effect at 10 h after stimulation with SP. We have also observed that HSV-1 cytopathic effect has not been significantly affected by pretreatment of macrophages with SP (2, 10 or 24 h before HSV infection). It can be concluded that the neurotransmitter SP has a significant role in modulating macrophage function and thus innate immunity processes in healthy and HSV-infected cells.
Yaraee
Roya
R
Department of Immunology, Medical Faculty, Shahed University, Tehran, Iran. yaraee@shahed.ac.ir
Ebtekar
Massoumeh
M
Ahmadiani
Abolhassan
A
Sabahi
Farzaneh
F
Ghazanfari
Tooba
T
D000818:Animals; D002522:Chlorocebus aethiops; D006561:Herpes Simplex / Q000378:metabolism* / Q000821:virology; D018259:Herpesvirus 1, Human; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism / Q000821:virology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000096:biosynthesis*; D009573:Nitrites / Q000378:metabolism; D013373:Substance P / Q000494:pharmacology*; D014709:Vero Cells
D009573:Nitrites; D009569:Nitric Oxide; D013373:Substance P