title: A new fermentation process allows large-scale production of tetra-N-acetyl-chitotetraosyl allosamizoline.
abstract: A new compound 2, possessing a tetra-N-acetyl-chitotetraosyl moiety as a constituent, was synthesized by bacterial fermentation, which used allosamizoline 1 as the initial acceptor. A 2-binding chitinase assay, indicated that the chitinase was inactivated by 2 with IC50 = 0.03 microg/mL.
authors.affiliation: School of Life Science, Shandong University, Jinan City 250100, China. hgl226@126.com
authors.forename: Gang-Liang
authors.initials: GL
authors.lastname: Huang
authors.forename: Xin-Ya
authors.initials: XY
authors.lastname: Mei
authors.forename: Hou-Cheng
authors.initials: HC
authors.lastname: Zhang
authors.forename: Peng-George
authors.initials: PG
authors.lastname: Wang
mesh_terms: D002236:Carbohydrate Conformation; D002688:Chitinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D005285:Fermentation; D005944:Glucosamine / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology; D011485:Protein Binding
chemical_list: D004791:Enzyme Inhibitors; C512151:tetra-N-acetyl-chitotetraosyl allosamizoline; D002688:Chitinases; D005944:Glucosamine