Disposition and metabolic profiling of bisphenol F in pregnant and nonpregnant rats.
The distribution of bisphenol F (4,4'-dihydroxydiphenyl-methane, BPF) was studied in female Sprague-Dawley rats. Pregnant and nonpregnant animals were gavaged with a single dose of 7 or 100 mg/kg [3H]BPF and were kept for 96 h in metabolic cages. The excretion of BPF residues occurred mainly in urine (43-54% of the administered dose), which was found to contain at least six different metabolites, and to a lesser extent in feces (15-20% of the administered dose). Sulfatase treatment and subsequent high-performance liquid chromatography analyses suggest that the major urinary metabolite (more than 50% of the radioactivity present in urine) is a sulfate conjugate of BPF. At 96 h, BPF residues were detectable in all tissues examined with the largest amounts in the liver (0.5% of the dose). In pregnant rats dosed at day 17 of gestation, BPF residues were detected in the uterus, placenta, amniotic fluid, and fetuses (0.9-1.3% of the administered dose). Large amounts of radioactivity (8-10% of the dose) were still located in the digestive tract lumen at the end of the study. After administration of a single oral dose of [3H]BPF, 46% of the distributed radioactivity was excreted in bile over a 6 h period. In rats, BPF and/or its metabolites very likely undergo enterohepatic cycling, which could be responsible for the relatively high amounts of residues still excreted 4 days after BPF administration. This bisphenol is efficiently absorbed and distributed to the reproductive tract in female rats, and its residues pass the placental barrier at a late stage of gestation in rats.
Cabaton
Nicolas
N
ENSBANA, UMR 1234 Toxicologie Alimentaire, 1 esplanade Erasme, 21000 Dijon, France.
Chagnon
Marie-Christine
MC
Lhuguenot
Jean-Claude
JC
Cravedi
Jean-Pierre
JP
Zalko
Daniel
D
D000818:Animals; D001559:Benzhydryl Compounds / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000652:urine; D001646:Bile / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004350:Drug Residues / Q000032:analysis*; D005260:Female; D005333:Fetus / Q000378:metabolism; D005506:Food Contamination; D010920:Placenta / Q000737:chemistry; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D014316:Tritium; D014599:Uterus / Q000737:chemistry; D000098833:Bisphenol F Compounds
D001559:Benzhydryl Compounds; D014316:Tritium; C008745:4,4'-bisphenol F; D000098833:Bisphenol F Compounds