title: Influence of interleukin-6 on the invasiveness of human colorectal carcinoma.
abstract: BACKGROUND
  Plasma interleukin-6 (IL-6) level correlates with patient survival in colorectal carcinoma and is regarded as a prognostic factor. In this study, the role of IL-6 in colorectal carcinoma proliferation, chemotaxis and invasion was investigated.
  
  
  METHODS
  Proliferation and invasion were measured in four colorectal carcinoma cell lines. The effect of IL-6 (10, 50, 100 ng/ml) on progression (the ability to grow, adhere, chemotax and invade) was also measured.
  
  
  RESULTS
  Physiological (10 ng/ml) and pharmacological (50 and 100 ng/ml) concentrations of IL-6 did not significantly affect growth. However, IL-6 (10 ng/ml) significantly increased attachment to basement membrane (p < 0.05). IL-6 (50 ng/ml) significantly increased the chemotaxis, anchorage-independent growth and invasiveness of SW-480 (one of the four cell lines) (p < 0.05). IL-6 (100 ng/ml) resulted in negative feedback inhibition of these effects.
  
  
  CONCLUSIONS
  IL-6 plays an important role in the progression of colorectal carcinoma.
authors.affiliation: Department of Medical Technology, Yuanpei University, Taiwan, ROC.
authors.forename: Chi-Pin
authors.initials: CP
authors.lastname: Hsu
authors.forename: Yuan-Chiang
authors.initials: YC
authors.lastname: Chung
mesh_terms: D002448:Cell Adhesion; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000473:pathology*; D018450:Disease Progression; D006801:Humans; D015850:Interleukin-6 / Q000097:blood / Q000502:physiology*; D009361:Neoplasm Invasiveness
chemical_list: D015850:Interleukin-6