Cinnamtannin B-1 from bay wood exhibits antiapoptotic effects in human platelets.
Proanthocyanidins, such as cinnamtannin B-1, are polyphenolic compounds with antioxidant activity that induce apoptosis in a number of tumoral cells. We have now investigated the pro- or anti-apoptotic effects of cinnamtannin B-1 in human platelets. Platelet stimulation with thrombin induced cellular apoptosis, as detected by phosphatidylserine exposure and the activation of caspases-3 and -9. Pretreatment for 30 min with cinnamtannin B-1 impaired thrombin-induced apoptosis in platelets. Thrombin has been shown to induce H(2)O(2) generation in platelets, which induced similar apoptotic events than thrombin in these cells. Pretreatment with cinnamtannin B-1 reduced H(2)O(2)-induced phosphatidylserine exposure and caspase activation. Finally, platelet stimulation with thrombin induced translocation of caspases-3 and -9 to the cytoskeletal (Triton-insoluble) fraction, which is important for their activation and the development of apoptotic events. Pretreatment with cinnamtannin B-1 impaired translocation of caspases-3 and -9 to the cytoskeleton and, as a result, procaspases are accumulated in the Triton-soluble fraction. Our results provide evidence for the antiapoptotic actions of cinnamtannin B-1 in human platelets.
Bouaziz
A
A
Unité de Recherche de Biochimie, Institut Supérieur de Biotechnologie 5019, Monastir, Tunisia.
Romera-Castillo
C
C
Salido
S
S
Linares-Palomino
P J
PJ
Altarejos
J
J
Bartegi
A
A
Rosado
J A
JA
Salido
G M
GM
D000872:Anthocyanins / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001792:Blood Platelets / Q000187:drug effects* / Q000502:physiology; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D002458:Cell Fractionation; D004789:Enzyme Activation; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D027442:Laurus / Q000737:chemistry*; D015394:Molecular Structure; D016877:Oxidants / Q000494:pharmacology; D010718:Phosphatidylserines / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry; D044945:Proanthocyanidins; D013917:Thrombin / Q000494:pharmacology
D000872:Anthocyanins; D016877:Oxidants; D010718:Phosphatidylserines; D010936:Plant Extracts; D044945:Proanthocyanidins; C517872:cinnamtannin B-1; D006861:Hydrogen Peroxide; D013917:Thrombin; D053148:Caspase 3; D053453:Caspase 9