[Identification of glial cell line-derived neurotrophic factor recombinant retroviral vector and establishment of its packaging cell line PA317].
OBJECTIVE
To identify glial cell line-derived neurotrophic factor (GDNF) recombinant retroviral vector and to establish its packaging cell line PA317.


METHODS
PA317 cells were transfected with recombinant retroviral vector pLXSN-GDNF using liposomes. The recombinant retroviral particles were then harvested from culture media of G418 resistant transfected cells and analyzed using RT-PCR. Virus titers in supernatants were investigated.


RESULTS
Sequencing date indicated that GDNF gene was exactly identical to the sequence in the GeneBank. PA317 cells were transfected with recombinant retroviral vector pLXSN-GDNF using liposomes, and virus titers in supernatants harvested from culture media of (G418 resistant transfected cells were 10(4)10(5) CFU/ml.


CONCLUSIONS
Packaging cell line PA317/pLXSN-GDNF was established.
Zhang
Wenjie
W
Department of Orthopaedics, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, P. R. China.
Li
Bingcang
B
Ren
Xianjun
X
D000818:Animals; D002478:Cells, Cultured; D042003:DNA Packaging; D005822:Genetic Vectors; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000235:genetics*; D051379:Mice; D041681:NIH 3T3 Cells; D011994:Recombinant Proteins; D012190:Retroviridae / Q000235:genetics*; D014162:Transfection
D051100:Glial Cell Line-Derived Neurotrophic Factor; D011994:Recombinant Proteins