Enrichment of phosphotyrosine proteome of human platelets by immunoprecipitation.
Proteomics offers the opportunity to comprehensively investigate the anucleate platelet. Here, we present a detailed procedure for enrichment by immunoprecipitation, using the monoclonal antibody 4G10, of the dynamic phosphotyrosine proteome of human platelets. Such an approach offers the possibility of capturing the dynamic tyrosine phosphorylation events that occur upon platelet activation and aggregation, with an aim to identify novel signaling proteins.
Foy
Martina
M
Department of Clinical Pharmacology, Royal College of Surgeons's in Ireland, Dublin, Ireland.
Harney
Donal F
DF
Wynne
Kieran
K
Maguire
Patricia B
PB
D001792:Blood Platelets / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods*; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000378:metabolism*; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics / Q000379:methods
D020543:Proteome; D019000:Phosphotyrosine