Growth arrest-inducing genes are activated in Dbl-transformed mouse fibroblasts.
The Dbl oncogene is a guanine nucleotide exchange factor for Rho GTPases and its activity has been linked to the regulation of gene transcription. Dbl oncogene expression in NIH3T3 cells leads to changes in morphological and proliferative properties of these cells, inducing a highly transformed phenotype. To gain insights into Dbl oncogene-induced transformation we compared gene expression profiles between Dbl oncogene-transformed and parental NIH3T3 cells by cDNA microarray. We found that Dbl oncogene expression is associated with gene expression modulation involving upregulation of 51 genes and downregulation of 49 genes. Five of the overexpressed genes identified are known to exert antiproliferative functions. Our observations suggest that the expression of Dbl oncogene in NIH3T3 may lead to the induction of genes associated with cell cycle arrest, possibly through the activation of stress-induced kinases.
Melani
Raffaella
R
Laboratorio di Biologia Molecolare, Istituto G. Gaslini, Largo G. Gaslini 5, 16147 Genova, Italy.
Sallustio
Fabio
F
Fardin
Paolo
P
Vanni
Cristina
C
Ognibene
Marzia
M
Ottaviano
Catherine
C
Melillo
Giovanni
G
Varesio
Luigi
L
Eva
Alessandra
A
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002453:Cell Cycle; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D004789:Enzyme Activation; D005347:Fibroblasts / Q000378:metabolism; D015870:Gene Expression / Q000502:physiology*; D020869:Gene Expression Profiling; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D041681:NIH 3T3 Cells; D020411:Oligonucleotide Array Sequence Analysis; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D020662:Guanine Nucleotide Exchange Factors; C512843:Mcf2 protein, mouse; D048051:p38 Mitogen-Activated Protein Kinases