Automated determination of flutamide by a validated flow-injection method: application to dissolution studies of pharmaceutical tablets.
The first flow-injection (FI) method for the determination of flutamide--a potent antiandrogen used for the treatment of prostate cancer--is reported. The method is based on the direct measurement of the absorbance of the analyte at 310 nm under flow conditions. Parameters affecting the determination such as detection wavelength, sample injection volume and flow rate were studied and optimized. The assay was validated (linearity, limits of detection and quantitation, accuracy, repeatability, reproducibility and selectivity) for the dissolution studies of flutamide-containing tablets during stability testing. The results were in good agreement with high performance liquid chromatography (HPLC) used as a reference method.
Tzanavaras
Paraskevas D
PD
Cosmopharm Ltd., Quality Control Department, P.O. Box 42, Korinthos 20100, Greece. paristzanavaras@gmail.com
Themelis
Demetrius G
DG
D000726:Androgen Antagonists / Q000032:analysis* / Q000737:chemistry; D001322:Autoanalysis; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D017022:Flow Injection Analysis / Q000295:instrumentation* / Q000379:methods*; D005485:Flutamide / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D008297:Male; D015394:Molecular Structure; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet; D013607:Tablets; D013997:Time Factors; D014867:Water / Q000737:chemistry
D000726:Androgen Antagonists; D013607:Tablets; D014867:Water; D012967:Sodium Dodecyl Sulfate; D005485:Flutamide