Immunochromatography using colloidal gold-antibody probe for the detection of atrazine in water samples.
An immunochromatography (ICG) strip test for rapid detection of atrazine in water samples was developed. A monoclonal antibody (MAb) specific to atrazine was produced from the cloned hybridoma cell (AT-1-M3) and used to develop a direct competitive enzyme-linked immunosorbent assay (DC-ELISA) and ICG strip. MAb conjugated to colloidal gold, and that was applied to the conjugate pad of the ICG strip. The visual detection limit for the ICG strip was 3 ng/mL. This test required only 10 min to get results and one step of sample to perform the assay. The results of water samples spiked with 5, 10, 20, and 50 ng/mL of atrazine by ICG strip were in good agreement with those obtained by DC-ELISA. The ICG strip was sufficiently sensitive and accurate to be useful for rapid screening of atrazine in various water samples.
Shim
Won-Bo
WB
Division of Applied Life Science (Brain Korea 21 program), Graduate School of Gyeongsang National University, Chinju, Gyeongnam 660-701, Korea.
Yang
Zheng-You
ZY
Kim
Ji-Young
JY
Choi
Jin-Gil
JG
Je
Jung-Hyun
JH
Kang
Sung-Jo
SJ
Kolosova
Anna Yu
AY
Eremin
Sergei A
SA
Chung
Duck-Hwa
DH
D000911:Antibodies, Monoclonal; D001280:Atrazine / Q000032:analysis*; D002845:Chromatography / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D017979:Gold Colloid; D006540:Herbicides / Q000032:analysis*; D007118:Immunoassay / Q000379:methods*; D014867:Water / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D017979:Gold Colloid; D006540:Herbicides; D014867:Water; D001280:Atrazine