title: Static normothermic preservation of renal allografts using a novel nonphosphate buffered preservation solution.
abstract: The aim of this study was to assess the viability and function of renal allografts under normothermic conditions using a novel nonphosphate buffered preservation solution AQIXRS-I. Porcine kidneys were flushed at 30 degrees C with AQIXRS-I at 100 mmHg pressure after 5-10 min warm ischaemic time and stored statically at either 4 degrees C or 30 degrees C for 2 h (n = 6 per group). Assessment of renal function by physiological and biochemical parameters was performed by perfusing the organs with autologous blood at 37 degrees C, with an initial circulating serum creatinine concentration of 1000 mumol/l on an isolated organ perfusion system for 6 h. Although the hypothermic group demonstrated overall superior renal function, the normothermic stored kidneys displayed a statistically comparable acid-base balance (7.37 +/- 0.15 vs. 7.3 +/- 0.09, P = 0.24). Furthermore, renal function was still evident after 6 h perfusion with increasing oxygen consumption, renal blood flow and reduced renal vascular resistance. The effectiveness and versatility of AQIXRS-I as a preservation solution under both normothermic and hypothermic conditions has been demonstrated. Renal viability was maintained after 2 h static normothermic storage. This study provides a foundation for further analysis utilizing normothermic preservation.
authors.affiliation: University Hospitals of Leicester, Leicester, UK. markuskayus@hotmail.com
authors.forename: Mark D
authors.initials: MD
authors.lastname: Kay
authors.forename: Sarah A
authors.initials: SA
authors.lastname: Hosgood
authors.forename: Simon J F
authors.initials: SJ
authors.lastname: Harper
authors.forename: Atul
authors.initials: A
authors.lastname: Bagul
authors.forename: Helen L
authors.initials: HL
authors.lastname: Waller
authors.forename: Douglas
authors.initials: D
authors.lastname: Rees
authors.forename: Michael L
authors.initials: ML
authors.lastname: Nicholson
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D003404:Creatinine / Q000097:blood; D007668:Kidney; D007677:Kidney Function Tests; D016030:Kidney Transplantation / Q000502:physiology*; D023421:Models, Animal; D019188:Organ Preservation Solutions; D013552:Swine; D014184:Transplantation, Homologous / Q000502:physiology
chemical_list: D019188:Organ Preservation Solutions; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D003404:Creatinine