Layer-by-layer assembly of biomacromolecules on poly(ethylene terephthalate) films and fiber fabrics to promote endothelial cell growth.
Poly(ethylene terephthalate) (PET) film and PET vascular graft were modified by layer-by-layer (LBL) assembly of collagen and chondroitin sulfate (CS) to mimic the natural composition and structure of extracellular matrix. PET materials were first hydrolyzed to endow the surfaces with negative charge. Alternative assembly of positively charged collagen and negatively charged CS was then conducted in an LBL manner to produce biological multilayers. X-ray photoelectron spectroscopy confirmed the occurrence of hydrolysis and existence of collagen and CS. The stepwise layer growth on the PET film was monitored by UV-vis spectroscopy. The multilayers had sufficient stability to pass through the cell culture period (8 days). In vitro endothelial cell culture revealed that the incorporated biomacromolecules could significantly improve the cell attachment, proliferation, and viability. Cells showed more spreading morphology with preserved phenotype, as illustrated by scanning electron microscopy and factor VIII-related antigen immunocytochemistry, respectively.
Liu
Yunxiao
Y
Key Laboratory of Macromolecule Synthesis and Functionalization of the Ministry of Education, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
He
Tao
T
Song
Haiqing
H
Gao
Changyou
C
D001808:Blood Vessels / Q000637:transplantation; D002021:Buffers; D002452:Cell Count; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000378:metabolism*; D003094:Collagen / Q000378:metabolism*; D042783:Endothelial Cells / Q000166:cytology* / Q000648:ultrastructure; D006801:Humans; D006868:Hydrolysis; D007231:Infant, Newborn; D011093:Polyethylene Terephthalates / Q000737:chemistry / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D014841:von Willebrand Factor / Q000378:metabolism
D002021:Buffers; D011093:Polyethylene Terephthalates; D014841:von Willebrand Factor; D002809:Chondroitin Sulfates; D003094:Collagen