title: Natural-abundance 15N--13C correlation spectra of vitamin B-12.
abstract: Two-dimensional nitrogen-carbon NMR correlation spectra have been derived by a new reconstruction technique based on standard two-dimensional HMQC and HMBC spectra, and operating with natural 15N and 13C isotopic abundances. Compared with conventional three-dimensional spectroscopy in which 15N and 13C spins must be present in the same molecule, the reconstruction method offers two orders of magnitude improvement in sensitivity. Vitamin B-12 serves as an illustrative example.
authors.affiliation: Varian, Ltd, 6 Mead Road, Yarnton, Oxford, UK.
authors.forename: Eriks
authors.initials: E
authors.lastname: Kupce
authors.forename: Ray
authors.initials: R
authors.lastname: Freeman
mesh_terms: D002247:Carbon Isotopes; D015394:Molecular Structure; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D014805:Vitamin B 12 / Q000737:chemistry*
chemical_list: D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D014805:Vitamin B 12