Antibacterial cathelicidin peptide CAP11 suppresses the anandamide production from lipopolysaccharide-stimulated mononuclear phagocytes.
The action of antibacterial cathelicidin peptide CAP11 on the anandamide production from mononuclear phagocytes was examined. Lipopolysaccharide (LPS)-stimulation induced the anandamide production from macrophage-like RAW264.7, accompanied with the enhanced anandamide-synthesizing enzyme activity; however, the anandamide-degrading enzyme activity was not changed by LPS-stimulation. Importantly, CAP11 suppressed the LPS-induced anandamide production and the increase of anandamide-synthesizing enzyme activity. Furthermore, CAP11 abrogated the LPS-binding to CD14-positive RAW264.7. These observations indicate that CAP11 inhibits the binding of LPS to CD14-positive mononuclear phagocytes, thereby suppressing the anandamide synthesizing enzyme activity and the anandamide production from the cells.
Murakami
Taisuke
T
Department of Host Defense and Biochemical Research, Juntendo University School of Medicine, 2-1-1 Hongo, Tokyo, Japan.
Yomogida
Shin
S
Someya
Akimasa
A
Kuwahara-Arai
Kyoko
K
Tamura
Hiroshi
H
Nagaoka
Isao
I
D000581:Amidohydrolases / Q000378:metabolism*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D001095:Arachidonic Acids / Q000096:biosynthesis*; D002460:Cell Line; D063388:Endocannabinoids; D004789:Enzyme Activation / Q000187:drug effects; D018950:Lipopolysaccharide Receptors / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000201:enzymology*; D051379:Mice; D053284:Polyunsaturated Alkamides; D011485:Protein Binding / Q000187:drug effects; D054804:Cathelicidins
D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D001095:Arachidonic Acids; D063388:Endocannabinoids; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D053284:Polyunsaturated Alkamides; D000581:Amidohydrolases; C087226:arachidonoylethanolamide synthase; C078814:anandamide; D054804:Cathelicidins