Cloning, expression and function of phosphate transporter encoded gene in Oryza sativa L.
OsPT6:1, a phosphate transporter encoding gene from the leaf samples of Oryza sativa, was identified through PCR with specifically designed primers. The phylogenetic analysis and the conserved amino acid residue site detection suggested OsPT6:1 a possible high-affinity phosphate transporter encoding gene. In situ hybridization and RT-PCR demonstrated the expression of OsPT6:1 in both roots and leaves. The peak expression signal was observed in mesophyll cells under low phosphorus (P) induction. A homologous recombination study indicated that OsPT6:1 can enhance the Pi uptake efficiency of Pichia pastoris. At the meantime, the introduction of OsPT6:1 was able to complement the Pi uptake function of yeast cells with high-affinity phosphate transporters deficient. Those results substantiated our contention that OsPT6:1 encoded a high-affinity phosphate transporter of Oryza sativa.
Ming
Feng
F
Institute of Genetics, State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai 200433, China. fming@fudan.edu.cn
Lu
Qun
Q
Wang
Wei
W
Zhang
Shanshan
S
Guo
Bin
B
Shen
Daleng
D
D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D028061:Phosphate Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D014158:Transcription, Genetic / Q000235:genetics
D028061:Phosphate Transport Proteins