Cre/loxP-mediated chromosome engineering of the mouse genome.
Together with numerous other genome modifications, chromosome engineering offers a very powerful tool to accelerate the functional analysis of the mammalian genome. The technology, based on the Cre/loxP system, is used more and more in the scientific community in order to generate new chromosomes carrying deletions, duplications, inversions and translocations in targeted regions of interest. In this review, we will present the basic principle of the technique either in vivo or in vitro and we will briefly describe some applications to provide highly valuable genetic tools, to decipher the mammalian genome organisation and to analyze human diseases in the mouse.
Brault
V
V
Institut de Transgénose, IEM, UMR6812, CNRS Uni-Orléans, 3B rue de la Férollerie, 45071 Orleans 2, France.
Besson
V
V
Magnol
L
L
Duchon
A
A
Hérault
Y
Y
D000818:Animals; D001287:Attachment Sites, Microbiological / Q000235:genetics*; D033481:Chromosomes, Mammalian / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D016678:Genome / Q000235:genetics*; D006801:Humans; D019426:Integrases / Q000378:metabolism*; D051379:Mice; D016296:Mutagenesis / Q000235:genetics
C045073:Cre recombinase; D019426:Integrases