Selective control of liver and kidney cells migration during organotypic cocultures inside fibronectin-coated rectangular silicone microchannels.
In this work, the behaviors of embryonic liver and kidney explants were studied inside rectangular polydimethylsiloxane (PDMS) microchannels. The organs were cultured under monoculture and coculture conditions on PDMS coated with or without fibronectin. The results demonstrated that the migration of cells from both organs is dependent on culture conditions and thus can be selectively controlled. In liver monocultures without fibronectin, cell migration in the microchannels resulted in the formation of a dense 3D tissue. Fibronectin reduced liver cell migration and enhanced the emergence of cells demonstrating typical hepatocyte phenotypes at the vicinity of the explant. The migration rate in liver-liver cocultures, with and without fibronectin, was roughly twice the rate of cells under monoculture conditions. In cocultures, both livers merged to form a large tissue in which the two initial organs could not be identified. In kidney monocultures, with and without fibronectin, we did not observe any migration inside the microchannels. Contrary to liver cells, kidney cell migration was triggered when both fibronectin coating and coculture with liver or another kidney explant were used. The migration was more largely observed in coculture with liver when compared to kidney-kidney cocultures. In the case of liver-kidney coculture with fibronectin, the progression of the kidney cells inside the microchannels appears as a displacement of the entire kidney explant in the direction of the liver. The liver cells did not move in those cases. After contact, we observed a complete merging of both liver and kidney explants. In contrast, for liver-kidney cocultures without fibronectin, only the liver moved toward the kidney.
Leclerc
Eric
E
CNRS-UMR 6600, Laboratoire de biomécanique et génie biomédical, Université de Technologie de Compiègne, BP 20529, 60205 Compiègne Cedex, France. eric.leclerc@utc.fr
Baudoin
Régis
R
Corlu
Anne
A
Griscom
Laurent
L
Luc Duval
Jean
J
Legallais
Cécile
C
D000818:Animals; D002465:Cell Movement / Q000502:physiology; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D018920:Coculture Techniques / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005353:Fibronectins / Q000737:chemistry / Q000494:pharmacology*; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects; D007668:Kidney / Q000166:cytology* / Q000187:drug effects; D008099:Liver / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development*; D019164:Liver, Artificial; D008422:Materials Testing; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D008846:Micromanipulation / Q000295:instrumentation / Q000379:methods; D009924:Organ Culture Techniques / Q000295:instrumentation* / Q000379:methods; D012828:Silicones / Q000737:chemistry; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods
D020099:Coated Materials, Biocompatible; D005353:Fibronectins; D012828:Silicones