Optimized dyeing conditions of immunoprotein with reactive dye Procion Blue MX-7RX.
For investigating the feasibility of using reactive dyes as an immunoassay marker, a dichlorine triazine dye, Procion Blue MX-7RX, was employed to stain the antibody against human serum albumin (anti-HSA). With the color intensity revealed in the immunochromatographic test strip as the objective variables, the optimal dyeing conditions were found as follows: pH 11.4, temperature 35.7 degrees C, molar ratio 188 (mol dye/mol antibody), and reaction time 45.6 min. The dyed-anti-HSA revealed a maximal color intensity of 8738 without apparent loss of antigen binding affinity.
Wang
Shwu-Jinng
SJ
Department of Chemical Engineering, National Chung Hsing University, Taichung 402, Taiwan, ROC.
Lin
Hsiu-Min
HM
Wang
Xin-Ren
XR
Hsiung
Kuang-Pin
KP
Liu
Yung-Chuan
YC
D000906:Antibodies / Q000737:chemistry*; D004396:Coloring Agents / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay; D012709:Serum Albumin / Q000032:analysis* / Q000276:immunology*; D013696:Temperature; D014227:Triazines / Q000737:chemistry*
D000906:Antibodies; D004396:Coloring Agents; D012709:Serum Albumin; D014227:Triazines; C036598:procion blue MX-R