Angiotensin II increases the cholesterol content of foam cells via down-regulating the expression of ATP-binding cassette transporter A1.
ATP-binding cassette transporter A1 (ABCA1) as one kind of membrane protein was found recently to play a major role in cholesterol homeostasis. Angiotensin II (AngII) has been shown to possess several atherogenic properties. The aim of the study is to investigate the influence of AngII on the expression of ABCA1 and the content of cholesterol in THP-1 derived foam cells. Our study showed that: (1) reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting demonstrated that AngII down-regulated the expression of ABCA1 in a dose-dependent manner. (2) The content of cholesterol was negatively correlated with ABCA1. The results suggest the promoting effects of AngII on the forming of foam cells are in a dose-dependent manner via down-regulating the expression of ABCA1.
Wang
Yanfu
Y
Laboratory of Cardiovascular Immunology, Institute of Cardiology, Union Hospital, Tong Ji Medical College, Hua Zhong University of Science and Technology, Wuhan, 430022, People's Republic of China.
Chen
Zhijian
Z
Liao
Yuhua
Y
Mei
Chunli
C
Peng
Hongyu
H
Wang
Min
M
Guo
Heping
H
Lu
Han
H
D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis*; D000804:Angiotensin II / Q000502:physiology*; D002784:Cholesterol / Q000378:metabolism*; D015536:Down-Regulation; D005487:Foam Cells / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D008854:Microscopy, Electron; D020133:Reverse Transcriptase Polymerase Chain Reaction
C577836:ABCA1 protein, human; D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters; D000804:Angiotensin II; D002784:Cholesterol