title: Apoptotic related biochemical changes in human amnion cells induced by tributyltin.
abstract: Tributyltin (TBT) is one of the environmental pollutants, which is mostly accumulated in marine animals. The toxic effects of TBT have been extensively documented in several types of cells, but the molecular mechanisms responsible for TBT-induced cell damage are still not fully elucidated. The present study was undertaken to evaluate the apoptotic related biochemical changes in human amnion cells induced by TBT. After cells were exposed to TBT at the concentrations of 1-4 microM for 2h, the results suggested that TBT could induce an early and typical apoptosis, moreover caspase-3, the modifications of cytoskeletal structure and the Bcl-2 family were involved in this process. The results will deepen our understanding about the toxic mechanism of TBT on human amnion cells.
authors.affiliation: Department of Biochemistry and Genetics, School of Medicine, Zhejiang University, Zhejiang, 310058 Hangzhou, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhu
authors.forename: Mingluan
authors.initials: M
authors.lastname: Xing
authors.forename: Jianlin
authors.initials: J
authors.lastname: Lou
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Wang
authors.forename: Wenyu
authors.initials: W
authors.lastname: Fu
authors.forename: Lihong
authors.initials: L
authors.lastname: Xu
mesh_terms: D000650:Amnion / Q000166:cytology* / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects*; D001345:Autoradiography; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D003599:Cytoskeleton / Q000187:drug effects / Q000648:ultrastructure; D005260:Female; D006801:Humans; D010718:Phosphatidylserines / Q000378:metabolism; D011247:Pregnancy; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D014220:Trialkyltin Compounds / Q000633:toxicity*; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis
chemical_list: D010718:Phosphatidylserines; D019253:Proto-Oncogene Proteins c-bcl-2; D014220:Trialkyltin Compounds; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein; C011559:tributyltin; D053148:Caspase 3