title: Site-specific functionalization of proteins by organopalladium reactions.
abstract: A new carbon-carbon bond has been regioselectively introduced into a target position (position 32 or 174) of the Ras protein by two types of organopalladium reactions (Mizoroki-Heck and Sonogashira reactions). Reaction conditions were screened by using a model peptide, and the stability of the Ras protein under the reaction conditions was examined by using the wild-type Ras protein. Finally, the iF-Ras proteins containing a 4-iodo-L-phenylalanine residue were subjected to organopalladium reactions with vinylated or propargylated biotin. Site-specific biotinylations of the Ras protein were confirmed by Western blot and LC-MS/MS.
authors.affiliation: Department of Biophysics and Biochemistry, School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Koichiro
authors.initials: K
authors.lastname: Kodama
authors.forename: Seketsu
authors.initials: S
authors.lastname: Fukuzawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakayama
authors.forename: Kensaku
authors.initials: K
authors.lastname: Sakamoto
authors.forename: Takanori
authors.initials: T
authors.lastname: Kigawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Yabuki
authors.forename: Natsuko
authors.initials: N
authors.lastname: Matsuda
authors.forename: Mikako
authors.initials: M
authors.lastname: Shirouzu
authors.forename: Koji
authors.initials: K
authors.lastname: Takio
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yokoyama
authors.forename: Kazuo
authors.initials: K
authors.lastname: Tachibana
mesh_terms: D013058:Mass Spectrometry; D008958:Models, Molecular; D010165:Palladium / Q000737:chemistry* / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D011506:Proteins; D010165:Palladium