dSLAM analysis of genome-wide genetic interactions in Saccharomyces cerevisiae.
Analysis of genetic interactions has been extensively exploited to study gene functions and to dissect pathway structures. One such genetic interaction is synthetic lethality, in which the combination of two non-lethal mutations leads to loss of organism viability. We have developed a dSLAM (heterozygote diploid-based synthetic lethality analysis with microarrays) technology that effectively studies synthetic lethality interactions on a genome-wide scale in the budding yeast Saccharomyces cerevisiae. Typically, a query mutation is introduced en masse into a population of approximately 6000 haploid-convertible heterozygote diploid Yeast Knockout (YKO) mutants via integrative transformation. Haploid pools of single and double mutants are freshly generated from the resultant heterozygote diploid double mutant pool after meiosis and haploid selection and studied for potential growth defects of each double mutant combination by microarray analysis of the "molecular barcodes" representing each YKO. This technology has been effectively adapted to study other types of genome-wide genetic interactions including gene-compound synthetic lethality, secondary mutation suppression, dosage-dependent synthetic lethality and suppression.
Pan
Xuewen
X
Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, MD 21205, USA.
Yuan
Daniel S
DS
Ooi
Siew-Loon
SL
Wang
Xiaoling
X
Sookhai-Mahadeo
Sharon
S
Meluh
Pamela
P
Boeke
Jef D
JD
D001483:Base Sequence; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal / Q000235:genetics / Q000502:physiology*; D005804:Genes, Lethal; D016678:Genome; D046228:Microarray Analysis / Q000379:methods*; D008969:Molecular Sequence Data; D009154:Mutation; D009841:Oligonucleotides / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D009841:Oligonucleotides