title: Isolation of four new pterocarpans from Zygophyllum eurypterum (Syn. Z. atriplicoides) with enzyme-inhibition properties.
abstract: Four new pterocarpans, atricarpan A (=(-)-1,2-dihydroxy-4-(hydroxymethyl)-3,9-dimethoxypterocarpan; 1), atricarpan B (=(-)-2,3-ethylenedioxy)-1,4-dihydroxy-9-methoxypterocarpan; 2), atricarpan C (=(-)-1,9-dimethoxypterocarpan-3-carboxylic acid; 3), and atricarpan D (=(-)-2,9-dimethoxy-4-(5-oxohexyl)pterocarpan; 4) were isolated from the BuOH extract of the whole plant of Zygophyllum eurypterum. The structure elucidations of those compounds were based primarily on 1D- and 2D-NMR analysis, including COSY, HMBC, and HMQC correlations. Compounds 1-4 also inhibited butyrylcholinesterase (BChE; EC 3.1.1.8) enzyme in a concentration-dependent manner with IC(50) values between 12.5-65.0 microM. Similarly, compounds 1 and 4 inhibited lipoxygenase (LOX; EC 1.13.11.12) and acetylcholinesterase (AChE; EC 3.1.1.7) enzymes with IC50 values of 13.5 and 20.5 muM, respectively.
authors.affiliation: H.E.J. Research Institute of Chemistry, International Center for Chemical Sciences, University of Karachi, Karachi-75270, Pakistan.
authors.forename: Viqar Uddin
authors.initials: VU
authors.lastname: Ahmad
authors.forename: Shazia
authors.initials: S
authors.lastname: Iqbal
authors.forename: Sarfraz Ahmad
authors.initials: SA
authors.lastname: Nawaz
authors.forename: M Iqbal
authors.initials: MI
authors.lastname: Choudhary
authors.forename: Umar
authors.initials: U
authors.lastname: Farooq
authors.forename: Syed Tariq
authors.initials: ST
authors.lastname: Ali
authors.forename: Amir
authors.initials: A
authors.lastname: Ahmad
authors.forename: Sadia
authors.initials: S
authors.lastname: Bader
authors.forename: Farzana
authors.initials: F
authors.lastname: Kousar
authors.forename: Saima
authors.initials: S
authors.lastname: Arshad
authors.forename: Rasool Bakhsh
authors.initials: RB
authors.lastname: Tareen
mesh_terms: D002091:Butyrylcholinesterase / Q000737:chemistry; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D016859:Lipoxygenase Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D015394:Molecular Structure; D036343:Pterocarpans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013329:Structure-Activity Relationship; D032445:Zygophyllum / Q000737:chemistry*
chemical_list: D002800:Cholinesterase Inhibitors; D016859:Lipoxygenase Inhibitors; D036343:Pterocarpans; D002091:Butyrylcholinesterase