[Effect of dimethoate on serum monoamines neurotransmitters in rats].
OBJECTIVE
To study the effect of dimethoate on the monoamine Neurotransmitters, including norepinephrine (NE), epinephrine (E), serotonin (5-HT), dopamine (DA) and its metabolite (3, 4-hydroxyphenylacetic acid, DOPAC) in the serum of rats and furthermore to explore the non-cholinergic mechanism of organophosphate induced toxicity.


METHODS
Groups of rats were treated with saline and 38.9, 83.7 and 180 mg/kg dimethoate respectively and were decapitated at the different time course from 0.5 to 24 hours after the administration. The monoamines neurotransmitters were determined by the reverse-phase high-performance liquid chromatography with the electrochemical detection.


RESULTS
The serum concentrations of DA (8.42% - 248.42% of the control), DOPAC (17.22% - 68.21% of the control) increased, according with the DM dosage and the exposure time, while the levels of NE (9.65% - 38.26% of the control) and E (11.00% - 32.62% of the control) contents decreased at the same time.


CONCLUSIONS
These findings indicate that dimethoate induced toxic effects can alter the monoamine levels at the different dosage and the time exposure in the serum of rats. It suggests that some non-cholinergic mechanisms may be involved in the dimethoate intoxication.
Ruan
Guo-Hong
GH
School of Public Health/Key Lab. of Public Health Safety, Ministry of Education, Fudan University, Shanghai 200032, China.
Wu
Qiang-En
QE
Gu
Ping
P
Zheng
Li-Xing
LX
Gu
Xi-An
XA
Zhou
Zhi-Jun
ZJ
D015102:3,4-Dihydroxyphenylacetic Acid / Q000097:blood; D000818:Animals; D015306:Biogenic Monoamines / Q000097:blood*; D004117:Dimethoate / Q000633:toxicity*; D004298:Dopamine / Q000097:blood; D004305:Dose-Response Relationship, Drug; D004837:Epinephrine / Q000097:blood; D008297:Male; D009638:Norepinephrine / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000097:blood
D015306:Biogenic Monoamines; D015102:3,4-Dihydroxyphenylacetic Acid; D012701:Serotonin; D004298:Dopamine; D004117:Dimethoate; D009638:Norepinephrine; D004837:Epinephrine