title: Isolation of pure compound R/J/3 from Pluchea indica (L.) Less. and its anti-amoebic activities against Entamoeba histolytica.
abstract: The plant Pluchea indica is known for its anti-inflammatory, anti-ulcer, anti-pyretic, hypoglycemic, diuretic and anti-microbial activities besides many other pharmacological activities. We have isolated and purified seven compounds from the methanolic root extract of this plant by column chromatography. The compounds were identified by spectroscopic analyses. The anti-amoebic activities of the pure compound R/J/3 was investigated against the HM1 strain of Entamoeba histolytica. The compound, R/J/3 showed the most pronounced anti-proliferative activity at a dose of 50 microg/ml. It also showed a marked activity on cell lysis of trophozoites, 4h after administration. The cell lytic activity was compared with metronidazole (5 microg/ml) as positive control.
authors.affiliation: Division of Pharmacology, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700 032, West Bengal, India.
authors.forename: Ria
authors.initials: R
authors.lastname: Biswas
authors.forename: P K
authors.initials: PK
authors.lastname: Dutta
authors.forename: B
authors.initials: B
authors.lastname: Achari
authors.forename: Durba
authors.initials: D
authors.lastname: Bandyopadhyay
authors.forename: Moumita
authors.initials: M
authors.lastname: Mishra
authors.forename: K C
authors.initials: KC
authors.lastname: Pramanik
authors.forename: T K
authors.initials: TK
authors.lastname: Chatterjee
mesh_terms: D000818:Animals; D000981:Antiprotozoal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004748:Entamoeba histolytica / Q000187:drug effects*; D015394:Molecular Structure; D013876:Thiophenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013997:Time Factors
chemical_list: C522935:2-(prop-1-ynyl)-5(5,6-dihydroxyhexa-1,3-diynyl)thiophene; D000981:Antiprotozoal Agents; D013876:Thiophenes