Gene expression patterns in Euglena gracilis: insights into the cellular response to environmental stress.
To better understand Euglena gracilis gene expression under different stress conditions (Chromium, Streptomycin or darkness), we undertook a survey of the E. gracilis transcriptome by cDNA sequencing and microarray analysis. First, we constructed a non-normalized cDNA library from the E. gracilis UTEX strain and sequenced a total of 1000 cDNAs. Six hundred and ten of these ESTs were similar to either Plantae or Protistae genes (e-value<e(-10)). Second, microarrays were built by spotting all the ESTs onto mirror slides. Microarray expression analysis indicated that 90 out of those 610 ESTs changed their expression level in response to different stress treatments (p<0.05). In addition, we detected 10 ESTs that changed expression levels irrespective of the tested stress. These may be considered as part of a larger set of stress-related genes in E. gracilis. Finally, we identified 23 unknown ESTs (U-ESTs) following the expression profiles of these putative stress-related genes suggesting that they could be related to the cellular mechanism of stress response.
Dos Santos Ferreira
Verónica
V
Laboratorio de Biología Molecular de la Enfermedad de Chagas INGEBI, National Research Council, Centro de Genómica Aplicada, University of Buenos Aires, Vuelta de Obligado 2490 2P, 1428, Buenos Aires, Argentina.
Rocchetta
Iara
I
Conforti
Visitación
V
Bench
Shellie
S
Feldman
Robert
R
Levin
Mariano J
MJ
D000818:Animals; D002857:Chromium / Q000494:pharmacology; D048028:DNA, Algal / Q000235:genetics; D018076:DNA, Complementary; D016054:DNA, Protozoan / Q000235:genetics; D003624:Darkness; D005056:Euglena gracilis / Q000235:genetics* / Q000502:physiology*; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000528:radiation effects*; D015723:Gene Library; D017125:Genes, Protozoan; D013307:Streptomycin / Q000494:pharmacology
D048028:DNA, Algal; D018076:DNA, Complementary; D016054:DNA, Protozoan; D002857:Chromium; D013307:Streptomycin