title: Immunohistochemical localization of estrogen receptors ERalpha and ERbeta in the spiny mouse (Acomys cahirinus) ovary during postnatal development.
abstract: This study was designed to determine the expression pattern of estrogen receptor (ER) subtypes in the Acomys cahirinus ovarian cells during its postnatal development. Immunohistochemical studies revealed the presence of ERalpha and ERbeta in germinal epithelium cells and interstitial tissue. Both these ER subtypes were also seen in granulosa cells and oocytes of growing follicles, however, the level of ERbeta expression was higher in comparison with ERalpha. In contrast to ERbeta, ERalpha protein was also present in theca cells. The expression of ERs increased with animals' age, but it decreased during follicular maturation. Moreover, the immunolocalization of ER subtypes in luteal cells showed that not ERbeta, but ERalpha expression is up-regulated throughout corpus luteum development. These immunohistochemical studies demonstrate, for the first time, that ERalpha is also expressed in the mouse granulosa cells and it may be a mediator of estrogen action in granulosa cells proliferation and differentiation.
authors.affiliation: Department of Comparative Anatomy and Anthropology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland. monhul@o2.pl
authors.forename: Monika
authors.initials: M
authors.lastname: Hułas-Stasiak
authors.forename: Antoni
authors.initials: A
authors.lastname: Gawron
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D003338:Corpus Luteum / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D047628:Estrogen Receptor alpha / Q000096:biosynthesis*; D047629:Estrogen Receptor beta / Q000096:biosynthesis*; D005260:Female; D006107:Granulosa Cells / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D051189:Murinae / Q000254:growth & development / Q000378:metabolism*; D009865:Oocytes / Q000166:cytology / Q000378:metabolism
chemical_list: D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta