title: A comparison between acetylcholine-like action potentials and square depolarizing pulses in triggering calcium entry and exocytosis in bovine chromaffin cells.
abstract: Depending on experimental conditions, cell model, and pattern and type of depolarizing stimuli, the relationship between calcium entry ([Ca2+]c) and the release of neurotransmitters and hormones varies from exponential (power of 3-4) to near linear (power of 1.5) or linear function. Here, we present a study using the more physiological stimulation pattern based on acetylcholine (ACh)-like action potentials, in voltage-clamped bovine chromaffin cells, with the perforated-patch configuration of the patch-clamp technique and 2 mM extracellular calcium. Trains of ACh-like action potentials or square depolarizing pulses of increasing length were applied, and calcium currents (ICa), total calcium entry (QCa), and exocytosis (DeltaCm) measured.
authors.affiliation: Instituto Teófilo Hernando, Departamento de Farmacología y Terapéutica, Facultad de Medicina, Universidad Autónoma de Madrid, 28029 Madrid, Spain.
authors.forename: A M
authors.initials: AM
authors.lastname: García de Diego
authors.forename: J J
authors.initials: JJ
authors.lastname: Arnáiz
authors.forename: L
authors.initials: L
authors.lastname: Gandía
authors.forename: J M
authors.initials: JM
authors.lastname: Hernández-Guijo
authors.forename: A G
authors.initials: AG
authors.lastname: García
mesh_terms: D000109:Acetylcholine / Q000502:physiology*; D000200:Action Potentials / Q000502:physiology*; D000311:Adrenal Glands / Q000502:physiology; D000818:Animals; D001692:Biological Transport; D002118:Calcium / Q000502:physiology*; D002417:Cattle; D019439:Chromaffin Cells / Q000502:physiology*; D005089:Exocytosis / Q000502:physiology*; D008564:Membrane Potentials / Q000502:physiology
chemical_list: D000109:Acetylcholine; D002118:Calcium