A comparison study in the proteomic signatures of multipotent germline stem cells, embryonic stem cells, and germline stem cells.
Germline stem (GS) cells can only differentiate into germline cells, while multipotent germ stem (mGS) cells, like embryonic stem (ES) cells, can differentiate into various somatic cells and tissues. The proteomic profiles in GS and mGS cells were compared by two-dimensional gel electrophoresis. Ten down-regulated and 16 up-regulated proteins were differentially expressed in mGS cells in comparison to GS cells, and these proteomic characteristics were very much similar to those in ES cells indicating that multipotency of mGS and ES cells is based on a common molecular event(s). Protein identification by mass spectrometry revealed that these proteins were functionally involved in cell signaling, transcription factors, metabolism, and protein folding. The identified proteins in the present study may thus reveal its biological characteristics and functional property in self-renewal and multipotency.
Kurosaki
Hajime
H
Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, Yonago, Tottori 683-8503, Japan.
Kazuki
Yasuhiro
Y
Hiratsuka
Masaharu
M
Inoue
Toshiaki
T
Matsui
Yasuhisa
Y
Wang
Chi Chiu
CC
Kanatsu-Shinohara
Mito
M
Shinohara
Takashi
T
Toda
Tosifusa
T
Oshimura
Mitsuo
M
D000818:Animals; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D005854:Germ Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D039902:Multipotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*
D020543:Proteome