title: Effects of brefeldin A on the localization of Tobamovirus movement protein and cell-to-cell movement of the virus.
abstract: It has been demonstrated that the subcellular location of Tobamovirus movement protein (MP) which was fused with green fluorescent protein (MP:GFP) changed during the infection process. However, the intracellular route through which MP is transported and its biological meaning are still obscure. Treatment with brefeldin A (BFA), which disrupts ER-to-Golgi transport, inhibited the formation of irregularly shaped and filamentous structures of MP. In this condition, MP was still targeted to plasmodesmata in leaf cells. Furthermore, the viral cell-to-cell movement was not inhibited by BFA treatment. These data indicated that the targeting of viral replication complexes (VRCs) to plasmodesmata is mediated by a BFA-insensitive pathway and that the ER-to-Golgi transport pathway is not involved in viral intercellular movement.
authors.affiliation: Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Komaba 3-8-1, Meguro, Tokyo 153-8902, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Tagami
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Watanabe
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D020126:Brefeldin A / Q000494:pharmacology*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism; D008124:Locomotion / Q000187:drug effects; D018515:Plant Leaves / Q000378:metabolism; D053830:Plant Viral Movement Proteins / Q000378:metabolism*; D014026:Nicotiana / Q000378:metabolism* / Q000821:virology; D017898:Tobamovirus / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000998:Antiviral Agents; D053830:Plant Viral Movement Proteins; D020126:Brefeldin A