title: [Analysis of 37 drugs in whole blood by HPLC after solid phase extraction].
abstract: OBJECTIVE
  To develop a specific, sensitive, reproducible SPE-HPLC method for the determination of 37 drugs in whole blood.
  
  
  METHODS
  With the doxapram as internal standard, Oasis column was used to extract drugs from whole blood. Two kinds of mobile phases were used in this study. Separations were achieved by a LiChrospher 100 RP-C18 (250 mm x 4.0 mm x 5 microm) column kept at 50 degrees C, the DAD detector was set at 230 nm and 250 nm.
  
  
  RESULTS
  The limit of detection were 1-30 ng/mL. The method showed excellent linearity and the linear correlation coefficient was > or =0.997 98. The relative standard deviation for between-day and within-day assay were <10%.
  
  
  CONCLUSIONS
  The method is effective, simple, reliable and has been used in real cases.
authors.affiliation: Shanghai Institute of Criminal Science and Technology, Shanghai 200083, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Liang
authors.forename: Yu-Rong
authors.initials: YR
authors.lastname: Zhang
authors.forename: Qi-Yun
authors.initials: QY
authors.lastname: Jin
authors.forename: You-Mei
authors.initials: YM
authors.lastname: Guo
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004315:Doxapram / Q000097:blood / Q000302:isolation & purification; D004316:Doxepin / Q000097:blood / Q000302:isolation & purification; D004949:Estazolam / Q000097:blood / Q000302:isolation & purification; D005554:Forensic Medicine; D006801:Humans; D009020:Morphine / Q000097:blood / Q000302:isolation & purification; D010208:Papaverine / Q000097:blood / Q000302:isolation & purification; D004364:Pharmaceutical Preparations / Q000097:blood* / Q000302:isolation & purification; D011224:Prazosin / Q000097:blood* / Q000302:isolation & purification; D011343:Procaine / Q000097:blood / Q000302:isolation & purification; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*; D012997:Solvents / Q000737:chemistry
chemical_list: D004364:Pharmaceutical Preparations; D012997:Solvents; D004316:Doxepin; D004949:Estazolam; D011343:Procaine; D009020:Morphine; D004315:Doxapram; D010208:Papaverine; D011224:Prazosin