Triterpenoids from Hippophae rhamnoides L. and their nitric oxide production-inhibitory and DPPH radical-scavenging activities.
In our investigation on the chemical constituents of Hippophae rhamnoides L., the chloroform-soluble fraction of the 80% acetone extract of branch bark was observed to inhibit nitric oxide (NO) production in a lipopolysaccharide and recombinant mouse interferon-gamma-activated murine macrophage-like cell line, RAW 264.7 cells. Two new triterpenoids, 2-O-trans-p-coumaroyl maslinic acid (1) and 2-O-caffeoyl maslinic acid (2), and three known triterpenoids, oleanolic acid (3), 3-O-trans-p-coumaroyl oleanolic acid (4), and 3-O-caffeoyl oleanolic acid (5), and 6-methoxy-2H-1-benzopyran (6) and beta-sitosterol (7) were isolated from the branch bark extract. Their inhibitory activities on the production of NO in RAW 264.7 cells and radical-scavenging activities were examined.
Yang
Zhi-Gang
ZG
China National Administration Center for Seabuckthorn Development, Ministry of Water Resources, Haidian District, Beijing 100038, P. R. China.
Li
He-Ran
HR
Wang
Li-Yan
LY
Li
Yong-Hai
YH
Lu
Shun-Guang
SG
Wen
Xiu-Feng
XF
Wang
Jue
J
Daikonya
Akihiro
A
Kitanaka
Susumu
S
D000818:Animals; D001713:Biphenyl Compounds / Q000737:chemistry*; D002460:Cell Line; D016166:Free Radical Scavengers / Q000494:pharmacology*; D031281:Hippophae / Q000737:chemistry*; D006834:Hydrazines / Q000737:chemistry*; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis*; D010851:Picrates; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*
D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D006834:Hydrazines; D010851:Picrates; D014315:Triterpenes; D009569:Nitric Oxide; C004931:1,1-diphenyl-2-picrylhydrazyl