A plant mutase that interconverts UDP-arabinofuranose and UDP-arabinopyranose.
Plant cell walls constitute the bulk of the earth renewable source of energy and are a component in the diet of humans and herbivores. l-Arabinofuranosyl (Araf) residues are a quantifiably important constituent of these walls. Plants use uridine diphosphate (UDP)-l-arabinofuranose (UDP-Araf) to donate Araf residues in the biosynthesis of Araf-containing polysaccharides, proteoglycans, and glycoproteins. However, little is known about the formation of UDP-Araf. We now describe the purification and partial characterization of a rice UDP-arabinopyranose mutase (UAM) that catalyzes the formation of UDP-Araf from UDP-arabinopyranose (UDP-Arap). The reaction is reversible and at thermodynamic equilibrium the pyranose form is favored over the furanose form (90 : 10). Three related proteins that are encoded by rice gene loci Os03g40270, Os04g56520, and Os07g41360 were identified from partial amino acid sequences of UAM. These proteins have >80% sequence identity with polypeptides that are reversibly glycosylated in the presence of UDP-sugars. The rice mutase and two functionally active recombinant mutases were shown to be reversibly glycosylated in the presence of UDP-Glc. The cofactor, flavin-adenine-dinucleotide (FAD), is required for the catalytic activity of UDP-galactose mutases of prokaryotes, fungi, and protozoa. The plant mutases, which do not require a cofactor, must therefore have a different catalytic mechanism. Putative UAM-encoding genes are present in the green algae Chlamydomonas reinhardtii, the moss Physcomitrella patens, the gymnosperm Pinus taeda (loblolly pine), and in numerous dicots and monocots, indicating that UAMs are widespread in green plants.
Konishi
Teruko
T
Forestry and Forest Products Research Institute, Tsukuba, Ibaraki, Japan.
Takeda
Takumi
T
Miyazaki
Yasumasa
Y
Ohnishi-Kameyama
Mayumi
M
Hayashi
Takahisa
T
O'Neill
Malcolm A
MA
Ishii
Tadashi
T
D000595:Amino Acid Sequence; D005182:Flavin-Adenine Dinucleotide / Q000737:chemistry; D017343:Genes, Plant; D006031:Glycosylation; D019751:Intramolecular Transferases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology* / Q000235:genetics; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D036226:Seedlings / Q000201:enzymology / Q000235:genetics; D014532:Uridine Diphosphate Glucose / Q000737:chemistry; D014539:Uridine Diphosphate Sugars / Q000737:chemistry* / Q000378:metabolism
D010940:Plant Proteins; D011994:Recombinant Proteins; D014539:Uridine Diphosphate Sugars; D005182:Flavin-Adenine Dinucleotide; C040817:uridine diphosphate arabinose; D019751:Intramolecular Transferases; D014532:Uridine Diphosphate Glucose