title: Mesenchymal stem cell-mediated functional tooth regeneration in swine.
abstract: Mesenchymal stem cell-mediated tissue regeneration is a promising approach for regenerative medicine for a wide range of applications. Here we report a new population of stem cells isolated from the root apical papilla of human teeth (SCAP, stem cells from apical papilla). Using a minipig model, we transplanted both human SCAP and periodontal ligament stem cells (PDLSCs) to generate a root/periodontal complex capable of supporting a porcelain crown, resulting in normal tooth function. This work integrates a stem cell-mediated tissue regeneration strategy, engineered materials for structure, and current dental crown technologies. This hybridized tissue engineering approach led to recovery of tooth strength and appearance.
authors.affiliation: Center for Craniofacial Molecular Biology, University of Southern California School of Dentistry, Los Angeles, California, United States of America; Department of Oral and Maxillofacial Rehabilitation, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Sonoyama
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Dianji
authors.initials: D
authors.lastname: Fang
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Yamaza
authors.forename: Byoung-Moo
authors.initials: BM
authors.lastname: Seo
authors.forename: Chunmei
authors.initials: C
authors.lastname: Zhang
authors.forename: He
authors.initials: H
authors.lastname: Liu
authors.forename: Stan
authors.initials: S
authors.lastname: Gronthos
authors.forename: Cun-Yu
authors.initials: CY
authors.lastname: Wang
authors.forename: Songlin
authors.initials: S
authors.lastname: Wang
authors.forename: Songtao
authors.initials: S
authors.lastname: Shi
mesh_terms: D000818:Animals; D003771:Dental Papilla / Q000166:cytology / Q000502:physiology; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D051379:Mice; D010513:Periodontal Ligament / Q000166:cytology / Q000502:physiology; D012038:Regeneration / Q000502:physiology*; D013552:Swine; D013556:Swine, Miniature; D014070:Tooth / Q000166:cytology / Q000502:physiology*; D014092:Tooth Root / Q000166:cytology / Q000502:physiology; D014183:Transplantation, Heterologous