Tissue factor-positive microparticles: cellular origin and association with coagulation activation in patients with colorectal cancer.
The pathogenesis of hypercoagulability in cancer is not entirely understood. We hypothesized that in cancer patients circulating tissue factor-positive microparticles (TF (+) MPs) are increased and associated with hemostatic system activation. In 20 patients with advanced colorectal cancer and in 20 age- and sex-matched controls, number and cellular origin of TF (+) MPs were determined in plasma by flow cytometry. D-dimer was determined as an indicator of hemostatic system activation. Compared to controls, the median (interquartile range) number of TF (+) MPs was two-fold higher in cancer patients: 25.9 (15.4 - 42.0) x 10 (3) /ml plasma versus 13.1 (11.9 - 19.7) x 10 (3) /ml plasma, p = 0.007. This was mainly due to a higher amount of TF (+) MPs from platelets (13.4 [5.0 - 17.4] x 10 (3) /ml plasma vs. 5.8 [4.5 - 7.5] x 10 (3) /ml plasma, p = 0.017). TF (+) MPs correlated with D-dimer ( ? = 0.48, p = 0.002). High levels of TF (+) MPs in cancer patients and their correlation with D-dimer suggest that TF (+) MPs might be involved in hemostasis activation in cancer patients.
Hron
Gregor
G
Dept. of Internal Medicine I, Medical University Vienna, Austria.
Kollars
Marietta
M
Weber
Heinz
H
Sagaster
Verena
V
Quehenberger
Peter
P
Eichinger
Sabine
S
Kyrle
Paul A
PA
Weltermann
Ansgar
A
D001777:Blood Coagulation; D002462:Cell Membrane / Q000737:chemistry*; D015179:Colorectal Neoplasms / Q000097:blood* / Q000150:complications; D005260:Female; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis; D005434:Flow Cytometry; D006487:Hemostasis; D006801:Humans; D008297:Male; D008875:Middle Aged; D010316:Particle Size; D019851:Thrombophilia / Q000209:etiology*; D013925:Thromboplastin / Q000032:analysis*
D005338:Fibrin Fibrinogen Degradation Products; C036309:fibrin fragment D; D013925:Thromboplastin