title: Caenorhabditis elegans SAND-1 is essential for RAB-7 function in endosomal traffic.
abstract: The small rab-GTPase RAB-7 acts in endosome and endosome to lysosome traffic. We identified SAND-1 as a protein required for RAB-7 function based on similarities between SAND-1 and RAB-7 RNAi phenotypes. Although the initial uptake of yolk protein in oocytes, or of soluble secreted (ss) GFP in coelomocytes, appeared normal, further transport along the endocytic traffic route was delayed in the absence of SAND-1 function, and yolk proteins failed to reach yolk granules efficiently. Moreover, in coelomocytes, ssGFP and BSA-Texas-Red were endocytosed but not transported to lysosomes. We show that SAND-1 is essential for RAB-7 function at the transition from early to late endosomes, but not for RAB-7 function at lysosomes.
authors.affiliation: Biozentrum, University of Basel, Klingelbergstrasse, Basel, Switzerland.
authors.forename: Dmitry
authors.initials: D
authors.lastname: Poteryaev
authors.forename: Hanna
authors.initials: H
authors.lastname: Fares
authors.forename: Bruce
authors.initials: B
authors.lastname: Bowerman
authors.forename: Anne
authors.initials: A
authors.lastname: Spang
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001692:Biological Transport; D017173:Caenorhabditis elegans; D029742:Caenorhabditis elegans Proteins / Q000502:physiology*; D002451:Cell Compartmentation; D003593:Cytoplasm / Q000378:metabolism; D004527:Egg Proteins / Q000378:metabolism; D004625:Embryo, Nonmammalian; D004705:Endocytosis; D011992:Endosomes / Q000378:metabolism*; D008566:Membranes / Q000378:metabolism; D020691:rab GTP-Binding Proteins / Q000502:physiology*; D020696:rab5 GTP-Binding Proteins / Q000378:metabolism; D000090802:rab7 GTP-Binding Proteins
chemical_list: D029742:Caenorhabditis elegans Proteins; D004527:Egg Proteins; C506114:SAND-1 protein, C elegans; D000090802:rab7 GTP-Binding Proteins; D020691:rab GTP-Binding Proteins; D020696:rab5 GTP-Binding Proteins