Ascorbic acid deficiency aggravates stress-induced gastric mucosal lesions in genetically scorbutic ODS rats.
We examined whether ascorbic acid (AA) deficiency aggravates water immersion restraint stress (WIRS)-induced gastric mucosal lesions in genetically scorbutic ODS rats. ODS rats received scorbutic diet with either distilled water containing AA (1 g/l) or distilled water for 2 weeks. AA-deficient rats had 12% of gastric mucosal AA content in AA-sufficient rats. AA-deficient rats showed more severe gastric mucosal lesions than AA-sufficient rats at 1, 3 or 6 h after the onset of WIRS, although AA-deficient rats had a slight decrease in gastric mucosal AA content, while AA-sufficient rats had a large decrease in that content. AA-deficient rats had more decreased gastric mucosal nonprotein SH and vitamin E contents and increased gastric mucosal lipid peroxide content than AA-sufficient rats at 1, 3 or 6 h of WIRS. These results indicate that AA deficiency aggravates WIRS-induced gastric mucosal lesions in ODS rats by enhancing oxidative damage in the gastric mucosa.
Ohta
Y
Y
Department of Chemistry, Fujita Health University, School of Medicine, Toyoake, Aichi, 470-1192, Japan. yohta@fujita-hu.ac.jp
Chiba
S
S
Imai
Y
Y
Kamiya
Y
Y
Arisawa
T
T
Kitagawa
A
A
D000818:Animals; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000378:metabolism; D001206:Ascorbic Acid Deficiency / Q000150:complications* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D005753:Gastric Mucosa / Q000378:metabolism / Q000473:pathology; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D011919:Rats, Inbred Strains; D012149:Restraint, Physical; D013315:Stress, Psychological / Q000150:complications* / Q000378:metabolism / Q000473:pathology; D013438:Sulfhydryl Compounds / Q000378:metabolism; D014810:Vitamin E / Q000378:metabolism
D008054:Lipid Peroxides; D013438:Sulfhydryl Compounds; D014810:Vitamin E; D001205:Ascorbic Acid