The effect of perfusion prior to cold preservation and addition of biliverdin on the liver graft from non-heart-beating donors.
OBJECTIVE
Our aim was to improve the energy status and viability of a liver graft from a non-heart-beating donor (NHBD), we investigated the effects of perfusion prior to cold preservation and the addition of an antioxidant, biliverdin.


METHODS
Rats were divided into five groups: group 1: without 30 minutes warm ischemia (WI) and cold preservation (control group); group 2 without WI and with 6 hours of cold preservation in UW solution (HBD group); group 3 with WI and cold preservation (NHBD group); group 4 with 30 minutes perfusion prior to cold preservation (PRE group); and group 5 with addition of biliverdin to precold preservation perfusion (BV group). Oxygenated Klebs-Henseleit solution was used as the perfusate prior to and after preservation. Portal flow and bile production during reperfusion, energy charge (EC), ATP level, GOT, and TNF-alpha were measured as well as a histological evaluation.


RESULTS
Portal flow of the PRE and BV groups during 1 hour of reperfusion was higher than of that the NHBD group. Bile production of the PRE group was also higher than that of the NHBD group, but bile production in the BV group was comparable to the NHBD group. EC of the PRE group was higher than that of the NHBD group prior to and after reperfusion. The EC and ATP levels of the BV group after reperfusion were higher than those of the NHBD and PRE groups. The GOT and TNF-alpha were reduced in the BV group.


CONCLUSIONS
Precold preservation perfusion improves the viability of grafts from NHBDs. Furthermore, biliverdin exerted an additive effect to ameliorate energy status.
Iwane
T
T
Division of Advanced Surgical Science and Technology, Tohoku University, Miyagi, Japan.
Akamatsu
Y
Y
Narita
T
T
Nakamura
A
A
Satomi
S
S
D000818:Animals; D001664:Biliverdine / Q000494:pharmacology*; D003080:Cold Temperature; D006323:Heart Arrest; D008099:Liver / Q000187:drug effects; D016031:Liver Transplantation / Q000502:physiology*; D008297:Male; D023421:Models, Animal; D009926:Organ Preservation / Q000379:methods*; D010477:Perfusion / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D014019:Tissue Donors
D001664:Biliverdine