Increased 8-OHdG levels in the urine, serum, and substantia nigra of hemiparkinsonian rats.
8-Hydroxy-2'-deoxyguanosine (8-OHdG), the predominant marker of oxidative DNA damage, may be a good biomarker for monitoring the progression of Parkinson's disease (PD). Unfortunately, there are no basic laboratory data examining 8-OHdG levels in animal models of PD. In this study, we demonstrate that rats lesioned with 6-hydroxydopamine (6-OHDA) in the medial forebrain bundle display significantly elevated 8-OHdG levels in urine, serum, and substantia nigra, but not cerebrospinal fluid and striatum, compared to sham controls. These increments in 8-OHdG levels were detected at 2 days, but not at 7 days after the lesion suggesting that oxidative stress is restricted to the acute phase of 6-OHDA neurotoxicity. The present results support 8-OHdG as a biomarker that may aid both in the diagnosis and in the documentation of progression in PD.
Yasuhara
Takao
T
Movement Disorders Program, Department of Neurology, Medical College of Georgia, 1429 Harper Street, HF-1121, Augusta, GA 30912, USA.
Hara
Koichi
K
Sethi
Kapil D
KD
Morgan
John C
JC
Borlongan
Cesario V
CV
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000378:metabolism / Q000652:urine; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism / Q000652:urine; D018450:Disease Progression; D009498:Neurotoxins; D018384:Oxidative Stress; D016627:Oxidopamine; D020734:Parkinsonian Disorders / Q000097:blood* / Q000503:physiopathology / Q000652:urine*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013378:Substantia Nigra / Q000378:metabolism* / Q000503:physiopathology; D013997:Time Factors; D015854:Up-Regulation
D015415:Biomarkers; D009498:Neurotoxins; D000080242:8-Hydroxy-2'-Deoxyguanosine; D016627:Oxidopamine; D003849:Deoxyguanosine