Detection of fetomaternal hemorrhage following chorionic villus sampling by Kleihauer Betke test and rise in maternal serum alpha feto protein.
OBJECTIVE
To assess incidence and volume of fetomaternal hemorrhage (FMH) after chorionic villus sampling (CVS) by Kleihauer Betke test (KBT) and rise in maternal protein (MSAFP).


METHODS
A prospective study was conducted on 61 cases requiring CVS. FMH due to CVS was assessed by KBT and MSAFP.


RESULTS
Out of 61 cases, 60 (98.36%) cases showed FMH by MSAFP while all showed FMH by KBT. Median volume of FMH detected by MSAFP was 0.06 mL (25 to 75th interquartile (IQR) 0.01 to 0.12 mL) and by KBT was 0.45 mL (25 to 75th IQR 0.09 to 0.90). Mean volume of FMH detected by MSAFP was 0.1 + 0.1424 mL and by KBT was 0.58 + 0.637 mL. Significant FMH up to 0.1 mL was found in 20 (32.8%) and 45 (60.6%) by MSAFP and KBT respectively.


CONCLUSIONS
MSAFP and KBT are sensitive to detect FMH. KBT estimates more FMH than MSAFP. Fifty micrograms of anti-D is sufficient to cover FMH during CVS.
Katiyar
Richa
R
All India Institute of Medical Sciences, Gynaecology and Obstetrics, India. dr.richa.katiyar@gmail.com
Kriplani
Alka
A
Agarwal
Nutan
N
Bhatla
Neerja
N
Kabra
Madhulika
M
D000328:Adult; D015193:Chorionic Villi Sampling / Q000009:adverse effects*; D005260:Female; D005319:Fetal Hemoglobin / Q000032:analysis*; D005331:Fetomaternal Transfusion / Q000097:blood / Q000175:diagnosis* / Q000209:etiology; D005865:Gestational Age; D006801:Humans; D011247:Pregnancy; D011446:Prospective Studies; D016037:Single-Blind Method; D000509:alpha-Fetoproteins / Q000032:analysis*
D000509:alpha-Fetoproteins; D005319:Fetal Hemoglobin