title: [The production of gastrodin through biotransformation of p-hydroxybenzaldehyde by cell suspension culture of Datura stramonium].
abstract: OBJECTIVE
  To investigate the production of p-hydroxymethylphenol-beta-D-glucoside (gastrodin) through biotransformation by plant cell suspension cultures.
  
  
  METHODS
  Using cell suspension cultures of Datura stramonium to convert the exogenous p-hydroxybenzaldehyde into gastrodin was conducted and the converted compounds were separated with a combination of multi-chromatography. Their chemical structures were determined on the basis of spectral analysis and chemical evidence.
  
  
  RESULTS
  The conversion procedure of p-hydroxybenzaldehyde into gastrodin by Datura stramonium cell suspension cultures was established. The synthesized gastrodin (II) was isolated from the fermental liquor and identified by spectral analysis. At the same time, the p-hydroxybenzyl alcohol (I) converted through biotransformation of p-hydroxybenzaldehyde by cell suspension cultures of Datura stramonium was also isolated and identified. Two compounds were also isolated from the cell cultures and they were identified as beta-D-furanoallulose (III) and n-butyloxystyryl-beta-D-pyranoallulose (IV).
  
  
  CONCLUSIONS
  Datura stramonium grown in suspension cultures can convert exogenous p-hydroxybenzaldehyde into the corresponding gastrodin.
authors.affiliation: Faculty of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China. gong199@163.com
authors.forename: Jia-Shun
authors.initials: JS
authors.lastname: Gong
authors.forename: Wei-Peng
authors.initials: WP
authors.lastname: Ma
authors.forename: Jun-Xue
authors.initials: JX
authors.lastname: Pu
authors.forename: Shu-Guan
authors.initials: SG
authors.lastname: Xu
authors.forename: Shuang-Qing
authors.initials: SQ
authors.lastname: Zheng
authors.forename: Chun-Jie
authors.initials: CJ
authors.lastname: Xiao
mesh_terms: D001547:Benzaldehydes / Q000378:metabolism*; D001592:Benzyl Alcohols / Q000302:isolation & purification / Q000378:metabolism; D001711:Biotransformation; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D013286:Datura stramonium / Q000166:cytology / Q000378:metabolism*; D005960:Glucosides / Q000096:biosynthesis* / Q000302:isolation & purification; D018547:Plant Stems / Q000166:cytology; D010946:Plants, Medicinal / Q000166:cytology / Q000378:metabolism
chemical_list: D001547:Benzaldehydes; D001592:Benzyl Alcohols; D005960:Glucosides; C018966:4-hydroxybenzyl alcohol; C045345:gastrodin; C011483:4-hydroxybenzaldehyde