title: Antioxidant capacity of crude extracts from clones of banana and plane species.
abstract: Banana and plane are the most important fruits in world trade, behind citric plants. In this work we studied the antioxidant capacity of banana and plane varieties of fruits obtained from interspecies crossed varieties of Musa acuminata and Musa balbisiana, named Harton plane, Cavendish banana, and Manzano banana. With this purpose we evaluated banana and plane crude extracts using the ferrous ion oxidation with xylenol orange method, the thiobarbituric acid method, determination of antioxidant activity, and effect on superoxide anion and hydroxyl radical and the radicals generated by ultraviolet light. The experiments showed that all extracts have the capacity to decrease the concentrations of lipid hydroperoxides and malondialdehyde, produced in the lipid peroxidation process, in a manner comparable to that of other widely studied antioxidants like melatonin and vitamin E. Moreover, all extracts had the capacity to inhibit the generation of superoxide anion, hydroxyl radical, and the radicals generated by ultraviolet light. When antioxidant activity was calculated, a value was found that was equivalent to a concentration of uric acid between 0.20 and 0.30 mM at the highest concentration of extract used, with uric acid being a potent antioxidant at 1 mM.
authors.affiliation: Laboratorio de Bioquímica Adaptativa, Departamento de Bioquímica, Facultad de Medicina, Universidad de Los Andes, Mérida, Mérida, Venezuela.
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Pérez-Pérez
authors.forename: Antonio J
authors.initials: AJ
authors.lastname: Rodríguez-Malaver
authors.forename: Nayalet
authors.initials: N
authors.lastname: Padilla
authors.forename: Gerardo
authors.initials: G
authors.lastname: Medina-Ramírez
authors.forename: Juan
authors.initials: J
authors.lastname: Dávila
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D005296:Ferrous Compounds / Q000737:chemistry; D005609:Free Radicals / Q000737:chemistry; D005638:Fruit / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000737:chemistry; D015227:Lipid Peroxidation / Q000187:drug effects; D028521:Musa / Q000737:chemistry*; D009243:NAD / Q000494:pharmacology; D010084:Oxidation-Reduction; D010636:Phenols; D010936:Plant Extracts / Q000494:pharmacology*; D013454:Sulfoxides; D013481:Superoxides / Q000737:chemistry; D017392:Thiobarbituric Acid Reactive Substances; D014466:Ultraviolet Rays; D014992:Xylenes / Q000737:chemistry
chemical_list: D000975:Antioxidants; D005296:Ferrous Compounds; D005609:Free Radicals; D010636:Phenols; D010936:Plant Extracts; D013454:Sulfoxides; D017392:Thiobarbituric Acid Reactive Substances; D014992:Xylenes; D009243:NAD; D013481:Superoxides; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide; C016833:xylenol orange