Anti-cyclooxygenase effects of lipid extracts from the New Zealand green-lipped mussel, Perna canaliculus.
Total lipid extracts of P. canaliculus (a bivalve marine mollusc native to New Zealand, commonly called the green-lipped mussel) and Mytilus edulis (commonly called the common blue mussel) moderately inhibited ovine COX-1 and COX-2 pure enzymes in vitro. The inhibition was increased after the mussel extracts were saponified by KOH hydrolysis. Protease- and protease-lipase-hydrolysed lipid extracts of P. canaliculus exhibited similarly strong COX inhibition as the KOH-hydrolysed extract. Lyprinol(R) (a commercial extract from P. canaliculus) also exhibited strong inhibition of both COX isoforms, an effect that was increased 10-fold upon subsequent hydrolysis. In contrast, fish oil was not as anti-COX active as Lyprinol. The Lyprinol free fatty acid fraction, and to a lesser extent the Lyprinol triglyceride fraction, were the only lipid classes of Lyprinol to exhibit strong inhibition of the COX isoforms. The purified PUFA extracts were all bioactive, potently inhibiting COX-1 and COX-2. Incubation of Lyprinol in the absence of exogenous arachidonic acid (AA) showed the appearance of alternate prostaglandin metabolites, confirming Lyprinol PUFA as a competitive substrate inhibitor of AA metabolism.
McPhee
S
S
Natural Products Research Group, School of Medical Sciences, Division of Laboratory Medicine, RMIT University, PO Box 71, Bundoora, Victoria 3083, Australia.
Hodges
L D
LD
Wright
P F A
PF
Wynne
P M
PM
Kalafatis
N
N
Harney
D W
DW
Macrides
T A
TA
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D016718:Arachidonic Acid / Q000378:metabolism; D005395:Fish Oils / Q000494:pharmacology*; D008401:Gas Chromatography-Mass Spectrometry; D007213:Indomethacin / Q000494:pharmacology; D008055:Lipids / Q000494:pharmacology*; D009520:New Zealand; D049883:Perna / Q000737:chemistry* / Q000378:metabolism; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D013997:Time Factors
D000893:Anti-Inflammatory Agents; D005395:Fish Oils; D008055:Lipids; C418997:lyprinol; D016718:Arachidonic Acid; D011451:Prostaglandin-Endoperoxide Synthases; D007213:Indomethacin