title: Growth-suppressive function of phosphatidylethanolamine-binding protein in anaplastic thyroid cancer.
abstract: BACKGROUND
  A cDNA microarray analysis of anaplastic thyroid cancer cell lines (ACL) was recently performed and the down-regulation of phosphatidylethanolamine-binding protein (PBP) [RAF kinase inhibitor protein (RKIP)] in ACL compared to normal thyroid tissues was identified.
  
  
  METHODS
  The expression levels of PBP in primary anaplastic and papillary thyroid cancer, thyroid cancer cell lines (anaplastic, papillary and follicular) and several normal human organs were examined. To examine the function of PBP, cell-growth assays were performed.
  
  
  RESULTS
  PBP expression was reduced in anaplastic thyroid cancers, compared to either normal thyroid tissues or differentiated thyroid cancers. PBP was expressed ubiquitously in normal human tissues. Exogenous PBP expression suppressed ACL growth, and suggested a tumor suppressive function of PBP in ACL.
  
  
  CONCLUSIONS
  This is the first report demonstrating that PBP may be a tumor suppressor whose loss is associated with development of anaplastic thyroid cancer from differentiated thyroid cancer.
authors.affiliation: Department of Molecular Biology, Institute of Gerontology, Nippon Medical School, 1-396, Kosugi-cho, Nakahara-ku, Kawasaki 211-8533, Japan. s4067@nms.ac.jp
authors.forename: Junko
authors.initials: J
authors.lastname: Akaishi
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Onda
authors.forename: Shinichi
authors.initials: S
authors.lastname: Asaka
authors.forename: Junichi
authors.initials: J
authors.lastname: Okamoto
authors.forename: Shizuyo
authors.initials: S
authors.lastname: Miyamoto
authors.forename: Mitsuji
authors.initials: M
authors.lastname: Nagahama
authors.forename: Kouichi
authors.initials: K
authors.lastname: Ito
authors.forename: Oichi
authors.initials: O
authors.lastname: Kawanami
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shimizu
mesh_terms: D045744:Cell Line, Tumor; D015536:Down-Regulation; D006801:Humans; D049488:Phosphatidylethanolamine Binding Protein / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013964:Thyroid Neoplasms / Q000473:pathology*
chemical_list: D049488:Phosphatidylethanolamine Binding Protein