Reduced apoptosis in human intestinal cells cured of persistent poliovirus infection.
Cells cured of persistent virus infection can be used to investigate cellular pathways of resistance to viral cytopathic effects. Persistent poliovirus (PV) infections were established in human intestinal Caco-2 cells, and spontaneously cured cell cultures were obtained. Two cell clones, cl6 and b13, cured of type 3 PV mutant infection and their parental Caco-2 cells were compared for susceptibility to PV infection, PV receptor CD155 expression, capacity to differentiate into polarized enterocytes, and PV-, staurosporine-, and actinomycin D-induced apoptosis. Our results strongly suggest that cells that are partially resistant to apoptosis can be selected during persistent virus infection.
Labadie
Karine
K
Virus Entérotropes et Stratégies Antivirales, Institut Pasteur, 25 rue du Dr. Roux, 75724 Paris Cedex 15, France.
Saulnier
Aure
A
Martin-Latil
Sandra
S
Colbère-Garapin
Florence
F
D017209:Apoptosis; D018938:Caco-2 Cells; D002454:Cell Differentiation / Q000187:drug effects; D002908:Chronic Disease; D002999:Clone Cells / Q000821:virology; D003609:Dactinomycin / Q000494:pharmacology; D004198:Disease Susceptibility; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D007422:Intestines / Q000166:cytology / Q000821:virology*; D008565:Membrane Proteins / Q000378:metabolism; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D011051:Poliomyelitis / Q000821:virology*; D017955:Poliovirus / Q000235:genetics / Q000502:physiology*; D011991:Receptors, Virus / Q000378:metabolism; D019311:Staurosporine / Q000494:pharmacology
D004791:Enzyme Inhibitors; D008565:Membrane Proteins; D019384:Nucleic Acid Synthesis Inhibitors; D011991:Receptors, Virus; C066100:poliovirus receptor; D003609:Dactinomycin; D019311:Staurosporine