FT-IR imaging of native and tissue-engineered bone and cartilage.
Fourier transform infrared (FT-IR) imaging and microspectroscopy have been extensively applied to the analyses of tissues in health and disease. Spatially resolved mid-IR data has provided insights into molecular changes that occur in diseases of connective or collagen-based tissues, including, osteoporosis, osteogenesis imperfecta, osteopetrosis and pathologic calcifications. These techniques have also been used to probe chemical changes associated with load, disuse, and micro-damage in bone, and with degradation and repair in cartilage. This review summarizes the applications of FT-IR microscopy and imaging for analyses of bone and cartilage in healthy and diseased tissues, and illustrates the application of these techniques for the characterization of tissue-engineered bone and cartilage.
Boskey
Adele
A
Hospital for Special Surgery and Weill Medical College and Graduate School of Medical Sciences of Cornell University, New York, NY 10021, USA. boskeya@hss.edu
Pleshko Camacho
Nancy
N
D000818:Animals; D001842:Bone and Bones / Q000033:anatomy & histology / Q000737:chemistry*; D002356:Cartilage / Q000033:anatomy & histology / Q000737:chemistry*; D003094:Collagen / Q000032:analysis; D003952:Diagnostic Imaging / Q000379:methods*; D006801:Humans; D011509:Proteoglycans / Q000032:analysis; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D023822:Tissue Engineering / Q000379:methods
D011509:Proteoglycans; D003094:Collagen