title: Cytotoxic triterpenoid saponins from the roots of Cephalaria gigantea.
abstract: Three new oleanane-type saponins, giganteosides L (1), M (2) and N (3) along with eight known ones were isolated from the roots of Cephalaria gigantea. Their structures were established as 3-O-[beta-D-galactopyranosyl-(1-->2)-beta-D-glucuronopyranosyl]-28-O-[beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranosyl]-oleanolic acid, 3-O-[beta-D-galactopyranosyl-(1-->2)-beta-D-glucuronopyranosyl]-28-O-[beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranosyl]-hederagenin, 3-O-[alpha-L-rhamnopyranosyl-(1-->2)-beta-D-glucuronopyranosyl]-28-O-[beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranosyl]-hederagenin, respectively, by means of spectroscopic methods (1D and 2D NMR, HR-ESI-MS). Cytotoxic activity of monodesmosides was investigated in vitro using three cancer cell lines, namely, human non pigmented melanoma MEL-5 and human leukemia HL-60. Giganteosides D (4) and E (5) showed antiproliferative effect on human cell lines with IC(50) values in the range 3.15-7.5 microM.
authors.affiliation: Institute of Pharmacochemistry, Academy of Sciences of Georgia, Tbilisi, Georgia.
authors.forename: Nino
authors.initials: N
authors.lastname: Tabatadze
authors.forename: Riad
authors.initials: R
authors.lastname: Elias
authors.forename: Robert
authors.initials: R
authors.lastname: Faure
authors.forename: Pascal
authors.initials: P
authors.lastname: Gerkens
authors.forename: Marie Claire
authors.initials: MC
authors.lastname: De Pauw-Gillet
authors.forename: Ether
authors.initials: E
authors.lastname: Kemertelidze
authors.forename: Aun
authors.initials: A
authors.lastname: Chea
authors.forename: Evelyne
authors.initials: E
authors.lastname: Ollivier
mesh_terms: D002240:Carbohydrate Sequence; D045744:Cell Line, Tumor; D049917:Dipsacaceae / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D018517:Plant Roots / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D021241:Spectrometry, Mass, Electrospray Ionization; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D012503:Saponins; D014315:Triterpenes