[Scanning aneugen and clastogen by micronuclei analysis using flow cytometry].
OBJECTIVE
To explore a flow cytometry (FCM)-based method for discriminating aneugen- or clastogen-induced micronuclei.


METHODS
Cells were stained with anti-CD71-FITC and PI, and the PI fluorescent signal intensity of micronucleated reticulocyte (MN-RET) in the peripheral blood of NIH mouse treated with COL or CP was detected by flow cytometry.


RESULTS
The ratio of the median of the intensity of MN-RET fluorescent signals to that of nucleated cell was low in the cyclophosphamide treated mouse, while the median was high in the colchicine treated mouse.


CONCLUSIONS
The flow cytometry-based micronucleus assay can be used to discriminate primarily smaller MN induced by the clastogen exposure from the larger MN induced by an aneugen.
Yang
Ming-Jie
MJ
Guangdong Centre for Disease Control and Prerention, Guangzhou 510300, China.
Zhou
Jian-Chang
JC
Li
Zhi
Z
Yang
Xing-Fen
XF
Huang
Jun-Ming
JM
Tan
Xiao-Hua
XH
Cao
Jia
J
Zeng
Rui-Ping
RP
D000818:Animals; D003078:Colchicine / Q000633:toxicity*; D003520:Cyclophosphamide / Q000633:toxicity*; D005434:Flow Cytometry / Q000379:methods; D008297:Male; D051379:Mice; D048629:Micronuclei, Chromosome-Defective; D009153:Mutagens / Q000633:toxicity; D012156:Reticulocytes / Q000187:drug effects* / Q000648:ultrastructure
D009153:Mutagens; D003520:Cyclophosphamide; D003078:Colchicine