Production of siRNA- and cDNA-transfected cell arrays on noncoated chambered coverglass for high-content screening microscopy in living cells.
In this chapter, we provide a protocol for the production of transfected cell arrays in living mammalian cells on noncoated chambered coverglass for the systematic functional analyses of human genes by high-content screening microscopy. This method should facilitate drug target validation by small-interfering RNAs.
Erfle
Holger
H
Cell Biology and Cell Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
Pepperkok
Rainer
R
D007091:Image Processing, Computer-Assisted; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D034741:RNA, Small Interfering; D013499:Surface Properties; D014162:Transfection
D034741:RNA, Small Interfering