title: Regulative mechanism of Ce3+ relieves DNA damage caused by Cd2+ in the kidney of silver crucian carp.
abstract: The mechanism of Cd2+ on the DNA cleavage and Ce3+ on the DNA repair in the kidney of silver crucian carp (Carassius auratus gibelio) is investigated by agarose gel electrophoresis methods and assaying biochemical indexes. It proves that Cd2+ induces the classical laddering degradation of DNA in vivo, but DNA cleavage is repaired after injecting with a low Ce3+ concentration under various Cd2+ concentrations. The DNA cleavage caused by Cd2+ is the result of the activation of deoxyribonuclease (DNase) and accumulation of reactive oxygen species (ROS), and Cd2+ destroys the antioxidant system, which diminishes the activities of superoxide dismutase, catalase, and peroxidase, and the increase of the lipid peroxidation (LPO) level. However, Ce3+ could inhibit activation of Cd2+ on DNase activity, relieve inhibition of Cd2+ on activities of the antioxidant enzyme, and diminish ROS accumulation. The results show that Ce3+ could relieve the toxicity of Cd2+ to silver crucian carp.
authors.affiliation: College of Life Sciences, Suzhou University, Suzhou 215006, People's Republic of China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Wu
authors.forename: Fashui
authors.initials: F
authors.lastname: Hong
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.forename: Yongling
authors.initials: Y
authors.lastname: Wang
authors.forename: Kang
authors.initials: K
authors.lastname: Wu
authors.forename: Fengqing
authors.initials: F
authors.lastname: Gao
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism; D002104:Cadmium / Q000378:metabolism / Q000633:toxicity*; D002347:Carps; D002586:Cesium / Q000378:metabolism*; D004249:DNA Damage; D003851:Deoxyribonucleases / Q000378:metabolism; D004583:Electrons; D004587:Electrophoresis, Agar Gel; D007477:Ions; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism*; D015227:Lipid Peroxidation; D008055:Lipids / Q000737:chemistry; D008670:Metals; D017382:Reactive Oxygen Species
chemical_list: D000975:Antioxidants; D007477:Ions; D008055:Lipids; D008670:Metals; D017382:Reactive Oxygen Species; D002104:Cadmium; D002586:Cesium; D003851:Deoxyribonucleases