title: SmaI cloning with regeneration of the SmaI site for sequential PCR product cloning.
authors.affiliation: Department of Bioengineering, Graduate School of Engineering, Osaka City University, Sugimoto 3-3-138, Sumiyoshi-ku, Osaka 558-8585, Japan. tatibana@bioa.eng.osaka-cu.ac.jp
authors.forename: Akira
authors.initials: A
authors.lastname: Tachibana
authors.forename: Toshizumi
authors.initials: T
authors.lastname: Tanabe
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Yamauchi
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D015257:DNA-Cytosine Methylases / Q000235:genetics* / Q000378:metabolism; D016678:Genome; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods*; D011994:Recombinant Proteins / Q000032:analysis
chemical_list: D017931:DNA Primers; D011994:Recombinant Proteins; C079006:DNA modification methylase XcyI; D015257:DNA-Cytosine Methylases