Functional characteristics of TauA binding protein from TauABC Escherichia coli system.
Although TauA shares few common characteristics with other known periplasmic binding protein, TauA is a putative periplasmic binding protein, part of tauABCD gene cluster involved in sulfonate transport in sulphate starvation condition. This protein was expressed in E. coli BL 21 and purified before to assess its binding functionalities. Measurement of K (d) value (mean 11.3 nM) by binding/dialysis studies revealed high affinity and specificity with taurine and also indicated that TauA possessed a unique binding site for its ligand. Comparisons with other periplasmic binding proteins suggests TauA plays a major role in ABC transport system and could be ideal candidate to serve as taurine catcher in biological fluids.
Javaux
Cédric
C
Biologie du Comportement, Université catholique de Louvain, Place Croix du Sud 1, 1348 Louvain-la-Neuve, Belgium.
Joris
Bernard
B
De Witte
Philippe
P
D018528:ATP-Binding Cassette Transporters / Q000737:chemistry* / Q000502:physiology; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial; D007700:Kinetics; D008024:Ligands; D026901:Membrane Transport Proteins / Q000737:chemistry; D033902:Periplasmic Binding Proteins / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D011489:Protein Denaturation; D017510:Protein Folding; D013654:Taurine / Q000737:chemistry
D018528:ATP-Binding Cassette Transporters; D029968:Escherichia coli Proteins; D008024:Ligands; D026901:Membrane Transport Proteins; D033902:Periplasmic Binding Proteins; C523384:TauA protein, E coli; D013654:Taurine