title: Pulmonary cytotoxicity of secondary metabolites of Stachybotrys chartarum (Ehrenb.) Hughes.
abstract: Damp dwellings represent suitable conditions for extended indoor moulds. A cellulolytic micromycete Stachybotrys chartarum (Ehrenb.) Hughes is considered to be a tertiary colonizer of surfaces in affected buildings. Known adverse health effects of S. chartarum result from its toxins--trichothecenes or atranones, as well as spirolactams. Mechanism of their potential pathological effects on the respiratory tract has not yet been sufficiently clarified. The cytotoxic effects of complex chloroform-extractable endo- (in biomass) and exometabolites (in cultivation medium) of an indoor S. chartarum isolate of an atranone chemotype, grown on a liquid medium with yeast extract and sucrose at 25 degrees C for 14 d, on lung tissue were evaluated in the 3-day experiment. For the purpose, 4 mg of toxicants were intratracheally instilled in 200 g Wistar male rats. A trichothecene mycotoxin diacetoxyscirpenol was used as the positive control. Bronchoalveolar lavage (BAL) parameters--viability and phagocytic activity of alveolar macrophages (AM), activity of lactate dehydrogenase, acid phosphatase and cathepsin D in cell-free BAL fluid (BALF), as well as in BAL cells, were measured. Acute exposure to the metabolites caused statistically significant changes, indicating lung tissue injury in the experimental animals. Decreased AM viability and increased activity of lysosomal enzyme cathepsin D in BAL cells after fungal exometabolite exposure were the most impressive. As toxic principles were found predominantly in the growth medium, toxins were more likely responsible for lung cell damage than e.g. fungal cell wall components. S. chartarum toxic metabolites can contribute to the ill health of occupants of mouldy building after inhalation of contaminated aerosol.
authors.affiliation: Slovak Medical University, Limbova 12, SK-83303 Bratislava, Slovakia. elena.pieckova@szu.sk
authors.forename: Elena
authors.initials: E
authors.lastname: Pieckova
authors.forename: Marta
authors.initials: M
authors.lastname: Hurbankova
authors.forename: Silvia
authors.initials: S
authors.lastname: Cerna
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Pivovarova
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Kovacikova
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D004195:Disease Models, Animal; D007322:Instillation, Drug; D008172:Lung Diseases, Fungal / Q000382:microbiology* / Q000473:pathology*; D016676:Macrophages, Alveolar / Q000737:chemistry; D008297:Male; D009183:Mycotoxins / Q000032:analysis / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D013191:Stachybotrys / Q000472:pathogenicity*; D018709:Statistics, Nonparametric
chemical_list: D009183:Mycotoxins