The S. cerevisiae Yap1 and Yap2 transcription factors share a common cadmium-sensing domain.
Towards elucidating the function of Yap2, which remains unclear, we have taken advantage of the C-terminal homology between Yap1 and Yap2. Swapping domains experiments show that the Yap2 C-terminal domain functionally substitutes for the homologous Yap1 domain in the response to Cd, but not to H2O2. We conclude that specificity determinants of the Cd response are encoded within both Yap1 and Yap2 C-terminus, whereas those required for H2O2 response are only present in the Yap1 C-terminus. Furthermore, our results identify FRM2 as Cd-responsive Yap2 target and indicate a possible role of this protein in regulating a metal stress response.
Azevedo
Dulce
D
Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Apartado 127, 2780-901 Oeiras, Portugal.
Nascimento
Liliana
L
Labarre
Jean
J
Toledano
Michel B
MB
Rodrigues-Pousada
Claudina
C
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D002104:Cadmium / Q000378:metabolism* / Q000633:toxicity; D003545:Cysteine / Q000737:chemistry / Q000235:genetics; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000633:toxicity; D028884:Karyopherins / Q000378:metabolism; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation; D000097606:Exportin 1 Protein
D002104:Cadmium; D003545:Cysteine; D006861:Hydrogen Peroxide; D028884:Karyopherins; D018160:Receptors, Cytoplasmic and Nuclear; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D000097606:Exportin 1 Protein; C082904:CAD1 protein, S cerevisiae; C086677:YAP1 protein, S cerevisiae