Negative cross-reactivity of rabbit anti-Malassezia furfur antibodies with other yeasts.
Anti-Malassezia furfur monospecific polyclonal antibodies was produced by repeated immunization of rabbit with Malassezia furfur yeast cells mixed with Freud adjuvant. The antibody titres of respective rabbit's serum samples prior to and after each immunization against M. furfur were assayed by indirect immunofluorescence technique using the M. furfur whole yeast antigen fixed in Teflon coated slides. The highest anti-M. furfur antibody titre achieved was 1 in 1280 dilution. At 1:20 dilution, none of the respective serum samples taken at various stages of immunization gave positive immunofluorescent staining against any of the other species of yeasts tested in this study. Anti-M. furfur monospecific polyclonal antibodies produced in rabbit in this study has the potential for diagnostic application in immunohistochemical detection of M. furfur in human tissues.
Chua
Kaw Bing
KB
International Medical University, Kuala Lumpur. chuakawbing@yahoo.com.sg
Devi
Shamala
S
Ng
Kee Peng
KP
Hooi
Poh Sim
PS
Na
Shiang Ling
SL
Chua
Kerk Hsiang
KH
D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology*; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D006801:Humans; D008290:Malassezia / Q000276:immunology*; D011817:Rabbits; D014010:Tinea Versicolor / Q000175:diagnosis; D015003:Yeasts / Q000276:immunology*
D000908:Antibodies, Fungal