Equal performance of TaqMan, MGB, molecular beacon, and SYBR green-based detection assays in detection and quantification of roundup ready soybean.
We have tested and compared the performance of 12 different assays representing four different real-time polymerase chain reaction (PCR) chemistries in the context of genetically modified organism detection. Several different molecular beacon, SYBR Green, TaqMan, and MGB assays were designed for the event specific detection and quantification of the 3' integration junction of GTS 40-3-2 (Roundup Ready) soybean. Sensitivity as well as robustness in the presence of background DNA were tested. None of the PCR-based approaches appeared to be significantly better than any of the other, but the molecular beacon assays had the lowest efficiency and also seemed more sensitive to changes in experimental setup.
Andersen
Charlotte Bøydler
CB
Section of Food and Feed Microbiology, National Veterinary Institute, Ullevaalsveien 68, 0454 Oslo, Norway.
Holst-Jensen
Arne
A
Berdal
Knut Gunnar
KG
Thorstensen
Tage
T
Tengs
Torstein
T
D018744:DNA, Plant / Q000032:analysis*; D005456:Fluorescent Dyes; D030821:Plants, Genetically Modified / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013025:Glycine max / Q000235:genetics*
D018744:DNA, Plant; D005456:Fluorescent Dyes