Effect of nitroglycerine on content of second messengers in myocytes of rat thoracic aorta.
We evaluated the concentration dependence and time dependence of the effect of nitroglycerine on intracellular content of cAMP, cGMP, and free Ca2+. It was shown that after norepinephrine stimulation, nitroglycerine exhibited calcium-blocking activity in lower concentrations (starting from 10(-7) M). Under conditions of experimental nitrate tolerance the dose-dependent effect of nitroglycerine on intracellular cGMP and Ca2+ was less pronounced. Calcium-blocking activity of nitroglycerine decreased most significantly upon stimulation of myocytes with norepinephrine.
Dukhanin
A S
AS
Russian State Medical University, Moscow.
Shimanovskii
N L
NL
D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology*; D002118:Calcium / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D032342:Muscle Cells / Q000378:metabolism*; D005996:Nitroglycerin / Q000494:pharmacology*; D009638:Norepinephrine; D051381:Rats; D015290:Second Messenger Systems / Q000187:drug effects* / Q000502:physiology; D013997:Time Factors; D014665:Vasodilator Agents / Q000494:pharmacology*
D014665:Vasodilator Agents; D000242:Cyclic AMP; D005996:Nitroglycerin; D006152:Cyclic GMP; D002118:Calcium; D009638:Norepinephrine