title: Synthesis and evaluation of polymeric continuous bed (monolithic) reversed-phase gradient stationary phases for capillary liquid chromatography and capillary electrochromatography.
abstract: There is a demand of novel high resolution separation media for separation of complex mixtures, particularly biological samples. One of the most flexible techniques for development of new separation media currently is synthesis of the continuous bed (monolithic) stationary phases. In this study the capillary format gradient stationary phases were formed using continuous bed (monolith) polymerization in situ. Different reversed-phase stationary phase gradients were tailored and their resolution using capillary liquid chromatography and capillary electrochromatography at isocratic mobile phase conditions was evaluated. It is demonstrated, that efficiency and resolution of the gradient stationary phases can be substantially increased comparing to the common (isotropic) stationary phases. The proposed formation approach of the gradient stationary phase is reproducible and compatible with the capillary format or microchip format separations. It can be easily automated for the separation optimizations or mass production of the capillary columns or chips.
authors.affiliation: Instituto di Metodologie Chimiche, Consiglio Nazionale delle Ricerche, Area della Ricerca di Roma I, Via Salaria Km 29,300, 00016 Monterotondo Scalo, Rome, Italy. a.maruska@gmf.vdu.lt
authors.forename: Audrius
authors.initials: A
authors.lastname: Maruska
authors.forename: Anna
authors.initials: A
authors.lastname: Rocco
authors.forename: Olga
authors.initials: O
authors.lastname: Kornysova
authors.forename: Salvatore
authors.initials: S
authors.lastname: Fanali
mesh_terms: D001555:Benzene Derivatives / Q000302:isolation & purification; D001565:Benzoates / Q000302:isolation & purification; D053801:Capillary Electrochromatography / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D046208:Microchip Analytical Procedures / Q000379:methods; D011108:Polymers / Q000138:chemical synthesis
chemical_list: D001555:Benzene Derivatives; D001565:Benzoates; D011108:Polymers