title: ESI mass spectrometry and X-ray diffraction studies of adducts between anticancer platinum drugs and hen egg white lysozyme.
abstract: The interactions of cisplatin and its analogues, transplatin, carboplatin and oxaliplatin, with hen egg white lysozyme were analysed through ESI mass spectrometry, and the resulting metallodrug-protein adducts identified; the X-ray crystal structure of the cisplatin lysozyme derivative, solved at 1.9 A resolution, reveals selective platination of imidazole Nepsilon of His15.
authors.affiliation: Department of Chemistry, University of Florence, Via della Lastruccia 3, 50019, Sesto Fiorentino, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Casini
authors.forename: Guido
authors.initials: G
authors.lastname: Mastrobuoni
authors.forename: Claudia
authors.initials: C
authors.lastname: Temperini
authors.forename: Chiara
authors.initials: C
authors.lastname: Gabbiani
authors.forename: Simona
authors.initials: S
authors.lastname: Francese
authors.forename: Gloriano
authors.initials: G
authors.lastname: Moneti
authors.forename: Claudiu T
authors.initials: CT
authors.lastname: Supuran
authors.forename: Andrea
authors.initials: A
authors.lastname: Scozzafava
authors.forename: Luigi
authors.initials: L
authors.lastname: Messori
mesh_terms: D000818:Animals; D001665:Binding Sites; D016190:Carboplatin / Q000737:chemistry / Q000378:metabolism; D002645:Chickens; D002945:Cisplatin / Q000031:analogs & derivatives / Q000737:chemistry* / Q000378:metabolism; D018360:Crystallography, X-Ray; D004529:Egg White; D008958:Models, Molecular; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism; D009944:Organoplatinum Compounds / Q000737:chemistry / Q000378:metabolism; D000077150:Oxaliplatin; D017671:Platinum Compounds / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D009944:Organoplatinum Compounds; D017671:Platinum Compounds; D000077150:Oxaliplatin; C034697:transplatin; D016190:Carboplatin; C421864:hen egg lysozyme; D009113:Muramidase; D002945:Cisplatin