title: Microbial contamination in dental unit waterlines: comparison between Er:YAG laser and turbine lines.
abstract: The investigation was carried out by evaluating the microbiological characteristics of the water before and after treatment with Er:YAG laser and turbine. The study was carried out in 2 dental surgeries. In both cases the laser and dental units were served by two independent circuits, fed by the same potable tap water. Samples were taken from the water supplying and the water leaving the turbine and laser before and after treatment on the same patient. Total heterotrophic plate count was measured at 36 degrees C and at 22 degrees C, and the presence of Staphylococcus species and non-fermenting Gram negative bacteria was investigated. Bacterial contamination was found within the circuit, especially in the laser device. Pseudomonas aeruginosa was detected in only 1 sample of supply water, in 11.1 % and in 19.4 % of the samples from the turbine and the laser respectively. No evidence of Staphylococcus aureus was found. The contamination of supply water was low, whereas that of the water leaving the handpieces of the 2 devices was high, especially in the laser. Attention should be paid to the control of the water leaving laser devices, given the increasingly wide use of such instruments in dental treatment exposed to risk of infection.
authors.affiliation: Dipartimento di Medicina e Sanita Pubblica, Sez. di Igiene, Via S. Giacomo, 12-40126 Bologna, Italy. rossella.sacchetti@unibo.it
authors.forename: Rossella
authors.initials: R
authors.lastname: Sacchetti
authors.forename: Augusto
authors.initials: A
authors.lastname: Baldissarri
authors.forename: Giovanna
authors.initials: G
authors.lastname: De Luca
authors.forename: Paola
authors.initials: P
authors.lastname: Lucca
authors.forename: Serena
authors.initials: S
authors.lastname: Stampi
authors.forename: Franca
authors.initials: F
authors.lastname: Zanetti
mesh_terms: D003666:Decontamination / Q000379:methods; D003754:Dental High-Speed Equipment / Q000382:microbiology*; D004203:Disinfection / Q000379:methods*; D004866:Equipment Contamination / Q000517:prevention & control*; D006801:Humans; D017053:Infection Control / Q000295:instrumentation; D007558:Italy; D007834:Lasers; D018709:Statistics, Nonparametric; D014871:Water Microbiology; D018508:Water Purification / Q000379:methods*