Sphingomyelin-cholesterol and double stranded RNA relationship in the intranuclear complex.
The nuclear double-stranded RNA can be exported to the cytoplasm leading to the incorporation alternative aminoacids into the translated protein, can be retained to the nucleus playing a role on quality control nuclear function or can engaged by vigilin complex initiating the heterochromatin function. In the nucleus this RNA is associated to the protein, a small amount of DNA, sphingomyelin, phosphatidylcholine, and enzymes related to sphingomyelin metabolism such as sphingomyelinase and sphingomyelin-synthase constituting an intranuclear complex. Our data show an association between cholesterol and sphingomyelin that could play a role in double strand formation after RNA synthesis since [3H]-uridine incorporation demonstrates that nuclear double stranded RNA is new-synthesized. The presence of the lamin B as a protein of the intranuclear complex suggests that it could correspond to the transcription sites associated to the inner nuclear membrane.
Rossi
Graziella
G
Department of Clinical and Experimental Medicine, Physiopathology, University School of Medicine, Policlinico Monteluce, via Brunamonti, 06100 Perugia, Italy.
Magni
Mariapia Viola
MV
Albi
Elisabetta
E
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism*; D005260:Female; D022781:Hepatocytes / Q000378:metabolism*; D008297:Male; D014176:Protein Biosynthesis / Q000502:physiology*; D012330:RNA, Double-Stranded / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013109:Sphingomyelins / Q000378:metabolism*
D012330:RNA, Double-Stranded; D013109:Sphingomyelins; D002784:Cholesterol