Helicobacter pylori cag pathogenicity island genes: clinical relevance for peptic ulcer disease development in Brazil.
The purpose of this study was to verify whether the presence of any of the Helicobacter pylori cagPAI genes or segments--cagA, cagA promoter, cagE, cagM, tnpB, tnpA, cagT and the left end of the cag II (LEC) region--would be a useful marker for the risk of peptic ulcer disease development. H. pylori DNA extracted from positive urease tests of 150 peptic ulcer patients and 65 dyspeptic controls was analysed by PCR. Duodenal ulcers were present in 110, gastric ulcers in 23 and both gastric and duodenal ulcers in 17 patients. A significant association (P <0.001) was found between a conserved cagPAI and peptic ulcer disease (34 %). The positivity of the cagA gene varied according to the region of the gene that was amplified. The region near to the promoter was present in almost all of the H. pylori isolates (97.2 %). The segment from nt 1764 to 2083 and the extreme right end were frequently deleted in the isolates from the controls (P <0.01). The positivity of the promoter region of cagA and cagT, cagE, cagM and LEC showed a significant difference between the isolates from peptic ulcer patients and from the controls (P <0.01). Patients usually had moderate gastritis; however, the intensity of the active inflammation was higher in the peptic ulcer group (P <0.001). cagT, cagM, LEC and the right end terminus of the cagA-positive H. pylori isolates were associated with a 27-fold, 8-fold, 4-fold and 4-fold risk of peptic ulcer disease, respectively, and may be useful markers to identify individuals at higher risk of peptic ulcer disease development in Brazil.
Mattar
Rejane
R
Department of Gastroenterology, University of São Paulo School of Medicine, São Paulo, SP, Brazil.
Marques
Sergio Barbosa
SB
Department of Gastroenterology, University of São Paulo School of Medicine, São Paulo, SP, Brazil.
Monteiro
Maria do Socorro
MDS
Department of Gastroenterology, University of São Paulo School of Medicine, São Paulo, SP, Brazil.
Dos Santos
Anibal Ferreira
AF
Department of Gastroenterology, University of São Paulo School of Medicine, São Paulo, SP, Brazil.
Iriya
Kiyoshi
K
Department of Gastroenterology, University of São Paulo School of Medicine, São Paulo, SP, Brazil.
Carrilho
Flair José
FJ
Department of Gastroenterology, University of São Paulo School of Medicine, São Paulo, SP, Brazil.
D000328:Adult; D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001938:Brazil; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005260:Female; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000235:genetics / Q000472:pathogenicity*; D006801:Humans; D008297:Male; D005810:Multigene Family / Q000235:genetics; D010437:Peptic Ulcer / Q000382:microbiology*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011706:Pyloric Antrum / Q000382:microbiology / Q000473:pathology; D012307:Risk Factors; D014774:Virulence / Q000235:genetics
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004269:DNA, Bacterial; C080866:cagA protein, Helicobacter pylori