title: An immunohistochemical and polymerase chain reaction evaluation of feline plasmacytic pododermatitis.
abstract: Sections of 14 skin biopsies of cats with plasmacytic pododermatitis and a clinical follow-up of 12-36 months were stained with a polyclonal anti-Mycobacterium bovis (Bacille Calmette-Guerin = BCG) antibody cross-reactive to a broad spectrum of fungi and bacteria. All sections were negative for organisms within the actual footpad tissue with the anti-BCG antibody stains. Polymerase chain reaction (PCR) assays that amplify the DNA of Bartonella spp., Ehrlichia spp., Anaplasma phagocytophilum, Chlamydophila felis, Mycoplasma spp., Toxoplasma gondii, and feline herpesvirus 1 (FHV-1) were applied to tissue digests. DNA of those pathogens assessed was not amplified from tissue.
authors.affiliation: Tierdermatologie Oberhaching, Oberhaching, Germany.
authors.forename: S V
authors.initials: SV
authors.lastname: Bettenay
authors.forename: M R
authors.initials: MR
authors.lastname: Lappin
authors.forename: R S
authors.initials: RS
authors.lastname: Mueller
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000378:metabolism / Q000382:microbiology / Q000469:parasitology / Q000473:pathology*; D002415:Cats; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005533:Foot Dermatoses / Q000382:microbiology / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D006090:Gram-Negative Bacteria / Q000235:genetics; D016905:Gram-Negative Bacterial Infections / Q000378:metabolism / Q000473:pathology / Q000662:veterinary; D006564:Herpesviridae / Q000235:genetics; D007150:Immunohistochemistry / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D014122:Toxoplasma / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D016054:DNA, Protozoan; D004279:DNA, Viral