title: Transcription factor serum response factor is selectively involved in the mechanisms of long-term synapse-specific plasticity.
abstract: Our previous studies demonstrated that acquisition of nociceptive sensitization in common snails is accompanied by long-term facilitation of the responses of defensive behavior command neurons LPl1 and RPl1 to sensory stimuli, this being dependent on the processes of translation and transcription. The mechanism of induction of long-term synaptic facilitation at the sensory inputs of neurons from chemoreceptors on the head involves cAMP and the immediate early gene transcription factor C/EBP (CCAAT-enhancer-binding protein), while regulation of the other sensory input of neurons LPl1 and RPl1 - from mechanoreceptors on the head - depends on protein kinase C. The present report describes studies of the involvement of the transcription factor serum response factor (SRF) in the processes of the synapse-specific plasticity of neuron LPl1 during the acquisition of sensitization in snails. The acquisition of sensitization during intracellular administration of oligonucleotides specifically inhibiting SRF led to the selective suppression of synaptic facilitation in the responses of neuron LPl1 to tactile stimulation of the snail's head. Synaptic facilitation of responses to chemical stimulation of the head and tactile stimulation of the foot developed just as in neurons in control sensitized animals. The results were assessed in relation to a hypothesis postulating that synapse-specific plasticity on learning may occur because of selective neurochemical "projection" of synaptic connections to various genes within neurons.
authors.affiliation: P. K. Anokhin Science Research Institute of Normal Physiology, Russian Academy of Medical Sciences, Building 4, 11 Mokhovaya Street, 103009 Moscow, Russia. nikitin.vp@mail.ru
authors.forename: V P
authors.initials: VP
authors.lastname: Nikitin
authors.forename: S A
authors.initials: SA
authors.lastname: Kozyrev
mesh_terms: D018712:Analgesics, Non-Narcotic / Q000494:pharmacology; D000818:Animals; D004347:Drug Interactions; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects / Q000502:physiology; D006257:Head / Q000033:anatomy & histology / Q000294:innervation; D006372:Helix, Snails; D066298:In Vitro Techniques; D009473:Neuronal Plasticity / Q000187:drug effects / Q000502:physiology*; D009475:Neurons, Afferent / Q000166:cytology* / Q000187:drug effects; D009841:Oligonucleotides / Q000494:pharmacology; D010812:Physical Stimulation / Q000379:methods; D011803:Quinine / Q000494:pharmacology; D026362:Serum Response Factor / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000502:physiology*; D013569:Synapses / Q000187:drug effects / Q000502:physiology*
chemical_list: D018712:Analgesics, Non-Narcotic; D009841:Oligonucleotides; D026362:Serum Response Factor; D011803:Quinine