A quantitative kinetic model for ATP-induced intracellular Ca2+ oscillations.
A quantitative kinetic model is proposed to simulate the ATP-induced intracellular Ca(2+) oscillations. The quantitative effect of ATP concentration upon the oscillations was successfully simulated. Our simulation results support previous experimental explanations that the Ca(2+) oscillations are mainly due to interaction of Ca(2+) release from the endoplasmic reticulum (ER) and the ATP-dependent Ca(2+) pump back into the ER, and the oscillations are prolonged by extracellular Ca(2+) entry that maintains the constant Ca(2+) supplies to its intracellular stores. The model is also able to simulate the sudden disappearance phenomenon of the Ca(2+) oscillations observed in some cell types by taking into account of the biphasic characteristic of the Ca(2+) release from the endoplasmic reticulum (ER). Moreover, the model simulation results for the Ca(2+) oscillations characteristics such as duration, peak [Ca(2+)](cyt), and average interval, etc., lead to prediction of some possible factors responsible for the variations of Ca(2+) oscillations in different types of cells.
Wang
Jinhui
J
Environmental Science Division, School of Earth and Space Science, University of Science and Technology of China, Hefei, Anhui Province 230026, China.
Huang
Xudong
X
Huang
Weidong
W
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003198:Computer Simulation; D004721:Endoplasmic Reticulum / Q000378:metabolism; D007424:Intracellular Fluid / Q000378:metabolism*; D008954:Models, Biological; D008956:Models, Chemical; D011493:Protein Kinase C / Q000378:metabolism; D015290:Second Messenger Systems / Q000502:physiology*; D010738:Type C Phospholipases / Q000378:metabolism
D000255:Adenosine Triphosphate; D011493:Protein Kinase C; D010738:Type C Phospholipases; D002118:Calcium