Synthesis and use of the protein phosphatase affinity matrices microcystin-sepharose and microcystin-biotin-sepharose.
Microcystin-based affinity matrices have been utilized to demonstrate the association of signaling proteins with protein phosphatases and for the purification of low-abundance microcystin-sensitive protein phosphatases. Here, we describe the procedure for the synthesis and use of microcystin-Sepharose and microcystin-biotin-Sepharose.
Moorhead
Greg B G
GB
Department of Biological Sciences, University of Calgary, Alberta, Canada.
Haystead
Timothy A J
TA
MacKintosh
Carol
C
D001710:Biotin / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods*; D052998:Microcystins / Q000737:chemistry*; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification; D010749:Phosphoprotein Phosphatases / Q000737:chemistry / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012685:Sepharose / Q000737:chemistry*
D052998:Microcystins; D010456:Peptides, Cyclic; D001710:Biotin; C078588:microcystin; D012685:Sepharose; D010749:Phosphoprotein Phosphatases