title: [Relationship between human age and mtDNA 4977 deletions in human blood cells].
abstract: OBJECTIVE
  To study the relationship between human age and mtDNA 4977 deletions in human blood cells.
  
  
  METHODS
  According to Anderson's sequence, two sets of primers were designed. Human mtDNA 4977 deletions in blood samples from 110 healthy human individuals in different age groups were examined by using Real time quantitative PCR.
  
  
  RESULTS
  The mtDNA 4977 deletions could be observed unless the age was under 23 years old. The older the people were, the easier the deletion could be observed.
  
  
  CONCLUSIONS
  mtDNA 4977 deletions in human blood cells were correlated with human ages in some extent.
authors.affiliation: Institute of Forensic Sciences, Ministry of Justice, P. R. China, Shanghai 200063, China. qzjdzx@163.com
authors.forename: Cheng-Tao
authors.initials: CT
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000375:Aging / Q000235:genetics*; D000704:Analysis of Variance; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D004252:DNA Mutational Analysis; D017931:DNA Primers / Q000235:genetics; D004272:DNA, Mitochondrial / Q000097:blood / Q000235:genetics*; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D017384:Sequence Deletion; D055815:Young Adult
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial; D005456:Fluorescent Dyes