Alternatively spliced human tissue factor (asHTF) is not pro-coagulant.
It has been proposed that alternatively-spliced human tissue factor (asHTF) is pro-coagulant. We have evaluated the function of asHTF in a mammalian expression system. Full-length human tissue factor (HTF) and asHTF were cloned from smooth muscle cells and over-expressed in HEK293 cells. As expected, a marked pro-coagulant activity (FX activation, thrombin generation) was observed on the surface, in lysates, and on microparticles from HTF transfected cells. In contrast, no pro-coagulant activity of asHTF was observed.
Censarek
Petra
P
Institut für Pharmakologie und Klinische Pharmakologie, Universitätsklinikum Düsseldorf, Germany.
Bobbe
Anett
A
Grandoch
Maria
M
Schrör
Karsten
K
Weber
Artur-Aron
AA
D017398:Alternative Splicing; D001777:Blood Coagulation / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D015951:Factor Xa / Q000032:analysis; D006801:Humans; D032389:Myocytes, Smooth Muscle / Q000737:chemistry; D013917:Thrombin / Q000096:biosynthesis; D013925:Thromboplastin / Q000235:genetics* / Q000502:physiology*; D014162:Transfection
D013925:Thromboplastin; D013917:Thrombin; D015951:Factor Xa