Apoptosis-associated gene expression in HIV-infected patients in response to successful antiretroviral therapy.
The simultaneous expression of 19 apoptosis-related genes was analyzed by RNA-protection assay in peripheral blood mononuclear cells of HIV-infected patients before and during successful antiretroviral therapy (ART). After 12 months of therapy, the expression of the pro-apoptotic genes FAS, FAS-L, FAF-1, FADD, CASPASE-8, DR3, TRAIL, TNFR-1, TRADD, and BAX was significantly downregulated with respect to time 0, while that of BCL-2, BCL-XL, and MCL-1 was significantly upregulated. The data suggest that inhibition of cell death in HIV-positive patients under successful therapy is the result of a complex network of multifactor signaling, correlated with both death and survival of lymphocytes.
Balestrieri
Emanuela
E
Department of Neuroscience, University of Rome Tor Vergata, Rome, Italy.
Grelli
Sandro
S
Matteucci
Claudia
C
Minutolo
Antonella
A
d'Ettorre
Gabriella
G
Di Sora
Fiorella
F
Montella
Francesco
F
Vullo
Vincenzo
V
Vella
Stefano
S
Favalli
Cartesio
C
Macchi
Beatrice
B
Mastino
Antonio
A
D019380:Anti-HIV Agents / Q000627:therapeutic use; D017209:Apoptosis; D004359:Drug Therapy, Combination; D005786:Gene Expression Regulation; D015658:HIV Infections / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology / Q000821:virology; D015497:HIV-1; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000502:physiology; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D018894:Reverse Transcriptase Inhibitors / Q000627:therapeutic use; D012260:Ribonucleases / Q000378:metabolism; D016896:Treatment Outcome
D019380:Anti-HIV Agents; D011506:Proteins; D018894:Reverse Transcriptase Inhibitors; D012260:Ribonucleases