title: Genotype and variations in core promoter and pre-core regions are related to progression of disease in HBV-infected patients from Northern Vietnam.
abstract: Vietnam is one of the countries with a high rate of hepatitis B virus (HBV) infection, but there are only a few reports about relation of HBV genotypes and mutations to clinical course in Northern Vietnam. The characteristics of HBV and its relationship to clinical outcome in patients from Northern Vietnam were analyzed. Serum samples were collected from 183 HBV-infected Vietnamese patients. They were clinically categorized into 4 groups: hepatocellular carcinoma (HCC), liver cirrhosis (LC), chronic hepatitis (CH), and asymptomatic carriers (ASC). HBV serology, alpha-fetoprotein, HBV genotypes, HBV-DNA level and mutations in the core promoter and pre-core regions of HBV-DNA were examined. The majority of sera contained HBV genotype B (67.8%) and C (27.9%). The median age was matched between genotype B and C (38.2 vs. 42.9 years). The rates of HBeAg seroconversion and G1896A for genotype B were significantly higher than those for genotype C (P<0.05). Genotype C had a higher HBV-DNA level than genotype B. C1858 was frequent, especially in genotype C (62.7%). The most prevalent genotype in ASC and CH was genotype B. The presence of the mutation A1762T/G1764A correlated with disease progression. The triple mutation T1753C/A1762T/ G1764A was quite common and was more prevalent in LC and HCC than in CH and ASC. In Northern Vietnamese, HBV genotypes B and C were prevalent. Genotype C and mutations in the core promoter region were associated with progressive, severe liver diseases.
authors.affiliation: Department of Clinical Molecular Medicine, Division of Diabetes, Digestive and Kidney Diseases, Kobe University Graduate School of Medicine, 751 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.
authors.forename: Bui Xuan
authors.initials: BX
authors.lastname: Truong
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Seo
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Yano
authors.forename: Pham Thi Thu
authors.initials: PT
authors.lastname: Ho
authors.forename: Tran Minh
authors.initials: TM
authors.lastname: Phuong
authors.forename: Dao Van
authors.initials: DV
authors.lastname: Long
authors.forename: Nguyen Truong
authors.initials: NT
authors.lastname: Son
authors.forename: Nguyen Cong
authors.initials: NC
authors.lastname: Long
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Kato
authors.forename: Yoshitake
authors.initials: Y
authors.lastname: Hayashi
authors.forename: Nguyen Khanh
authors.initials: NK
authors.lastname: Trach
authors.forename: Masato
authors.initials: M
authors.lastname: Kasuga
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D004279:DNA, Viral / Q000235:genetics; D018450:Disease Progression; D005260:Female; D014644:Genetic Variation / Q000235:genetics*; D005838:Genotype; D006509:Hepatitis B / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology* / Q000628:therapy; D006513:Hepatitis B e Antigens / Q000097:blood; D006515:Hepatitis B virus / Q000145:classification / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011401:Promoter Regions, Genetic / Q000235:genetics*; D016896:Treatment Outcome; D014744:Vietnam / Q000453:epidemiology; D000509:alpha-Fetoproteins / Q000378:metabolism
chemical_list: D004279:DNA, Viral; D006513:Hepatitis B e Antigens; D000509:alpha-Fetoproteins