Coexpression and spatial association of nicotinic acetylcholine receptor subunits alpha7 and alpha10 in rat sympathetic neurons.
Fast excitatory synaptic transmission in sympathetic ganglia is mediated by nicotinic acetylcholine receptors (nAChRs). Although it is known that the nAChR alpha7-subunit occurs in sympathetic ganglia, the expression of the recently cloned subunit alpha10 (Elgoyhen et al., 2001; Lustig et al., 2001; Sgard et al., 2002) has not been analyzed. Until now, functional receptors containing alpha10-subunits have been found only in combination with alpha9-subunits (Elgoyhen et al., 2001; Lustig et al., 2001; Sgard et al., 2002). The alpha9-subunit exhibits a restricted expression pattern, whereas the alpha10-subunit is expressed more widely. This broad distribution resembles more closely that known for subunit alpha7 than for subunit alpha9. On this background, we investigated the distribution of nAChR subunits alpha7, alpha9, and alpha10 in rat sympathetic ganglia and studied a possible interaction between subunit alpha7 and potential partners by double-labeling immunofluorescence and fluorescence resonance energy transfer (FRET) (Kam et al., 1995; Jares-Erijman and Jovin, 2003).
Lips
Katrin Susanne
KS
Institute for Anatomy and Cell Biology and University of Giessen Lung Center, Justus-Liebig-University, 35385 Giessen, Germany. Katrin.S.Lips@anatomie.med.uni-giessen.de
König
Peter
P
Schätzle
Katrin
K
Pfeil
Uwe
U
Krasteva
Gabriela
G
Spies
Markus
M
Haberberger
Rainer Viktor
RV
Grando
Sergei A
SA
Kummer
Wolfgang
W
D000818:Animals; D005728:Ganglia, Sympathetic / Q000502:physiology*; D017403:In Situ Hybridization; D009474:Neurons / Q000502:physiology*; D021122:Protein Subunits / Q000737:chemistry* / Q000235:genetics*; D051381:Rats; D011978:Receptors, Nicotinic / Q000737:chemistry* / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D064569:alpha7 Nicotinic Acetylcholine Receptor
C470699:Chrna10 protein, rat; C579165:Chrna7 protein, rat; D021122:Protein Subunits; D011978:Receptors, Nicotinic; D064569:alpha7 Nicotinic Acetylcholine Receptor