Natural-abundance 15N--13C correlation spectra of vitamin B-12.
Two-dimensional nitrogen-carbon NMR correlation spectra have been derived by a new reconstruction technique based on standard two-dimensional HMQC and HMBC spectra, and operating with natural 15N and 13C isotopic abundances. Compared with conventional three-dimensional spectroscopy in which 15N and 13C spins must be present in the same molecule, the reconstruction method offers two orders of magnitude improvement in sensitivity. Vitamin B-12 serves as an illustrative example.
Kupce
Eriks
E
Varian, Ltd, 6 Mead Road, Yarnton, Oxford, UK.
Freeman
Ray
R
D002247:Carbon Isotopes; D015394:Molecular Structure; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D014805:Vitamin B 12 / Q000737:chemistry*
D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D014805:Vitamin B 12