title: Automated laser scanning cytometry: a powerful tool for multi-parameter analysis of drug-induced apoptosis.
abstract: BACKGROUND
  Simultaneous analysis of multiple intracellular events is critical for assessing the effect of biological response modifiers, including the efficacy of chemotherapy. Here we used the automated laser scanning cytometry (LSC) for multi-parameter analysis of drug-induced tumor cell apoptosis.
  
  
  METHODS
  Using 2-mercaptopyridine-N-oxide-hydrate sodium salt, or the commonly used chemotherapeutic agents etoposide and camptothecin, we performed simultaneous analyses of apoptosis-related morphological features as well as fluorescence-based biochemical changes in a 96-well format.
  
  
  RESULTS
  We demonstrate the scope of LSC as a platform for comparing multiple variables between different cell populations, distinguishing unique events at a single cell level within a sample population, and enabling simultaneous screenings in a single assay at multiple dosages and time-points.
  
  
  CONCLUSIONS
  These data underscore the power of LSC for simultaneous multi-parameter analysis, which could have implications for screening or assessing the efficacy of drug responses in heterogeneous cell populations and at the single cell level.
authors.affiliation: ROS Biology and Apoptosis Group, National University Medical Institutes, Singapore.
authors.forename: Andrea Lisa
authors.initials: AL
authors.lastname: Holme
authors.forename: Sanjiv Kumar
authors.initials: SK
authors.lastname: Yadav
authors.forename: Shazib
authors.initials: S
authors.lastname: Pervaiz
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis; D016466:CHO Cells; D002166:Camptothecin / Q000494:pharmacology; D002463:Cell Membrane Permeability / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D053938:DNA Fragmentation; D005047:Etoposide / Q000494:pharmacology; D047569:Laser Scanning Cytometry / Q000379:methods*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D011725:Pyridines / Q000494:pharmacology; D013871:Thiones / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D011725:Pyridines; D013871:Thiones; C008704:pyrithione; D005047:Etoposide; D002166:Camptothecin