Recommended criteria for the mass spectrometric identification of target peptides and proteins (<8 kDa) in sports drug testing.
Mass spectrometry has become an invaluable tool for the identification of prohibited peptide hormones and proteins in doping control analysis. Regulatory authorities have established criteria for identifying banned drugs in doping control specimens, but these criteria do not address the specific issues for high molecular weight protein drugs such as molecular weight determination of multiply charged molecules, analysis of chemically or enzymatically derived degradation products, identification of amino acid sequence tags, etc. Technical considerations such as sample preparation methods (e.g. immunoaffinity purification), resulting analytes (e.g. intact compounds vs. chemically or enzymatically derived peptides), ionization modes, analyzer resolution, and the information provided by respective techniques are discussed in light of sports drug testing requirements using typical application examples.
Thevis
Mario
M
Institute of Biochemistry-Center for Preventive Doping Research, German Sport University Cologne, Carl-Diem Weg 6, 50933 Cologne, Germany. m.thevis@biochem.dshs-koeln.de
Loo
Joseph A
JA
Loo
Rachel R Ogorzalek
RR
Schänzer
Wilhelm
W
D004300:Doping in Sports / Q000517:prevention & control; D017408:Guidelines as Topic; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods* / Q000592:standards*; D008832:Microchemistry / Q000379:methods / Q000592:standards; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis* / Q000737:chemistry; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D013048:Specimen Handling / Q000379:methods / Q000592:standards; D015813:Substance Abuse Detection / Q000379:methods* / Q000592:standards*; D014481:United States
D010455:Peptides; D011506:Proteins