title: Quantitative proteomics analysis of the secretory pathway.
abstract: We report more than 1400 proteins of the secretory-pathway proteome and provide spatial information on the relative presence of each protein in the rough and smooth ER Golgi cisternae and Golgi-derived COPI vesicles. The data support a role for COPI vesicles in recycling and cisternal maturation, showing that Golgi-resident proteins are present at a higher concentration than secretory cargo. Of the 1400 proteins, 345 were identified as previously uncharacterized. Of these, 230 had their subcellular location deduced by proteomics. This study provides a comprehensive catalog of the ER and Golgi proteomes with insight into their identity and function.
authors.affiliation: Department of Anatomy and Cell Biology, McGill University, 3640 University Street, Montreal, Quebec H3A 2B2, Canada.
authors.forename: Annalyn
authors.initials: A
authors.lastname: Gilchrist
authors.forename: Catherine E
authors.initials: CE
authors.lastname: Au
authors.forename: Johan
authors.initials: J
authors.lastname: Hiding
authors.forename: Alexander W
authors.initials: AW
authors.lastname: Bell
authors.forename: Julia
authors.initials: J
authors.lastname: Fernandez-Rodriguez
authors.forename: Souad
authors.initials: S
authors.lastname: Lesimple
authors.forename: Hisao
authors.initials: H
authors.lastname: Nagaya
authors.forename: Line
authors.initials: L
authors.lastname: Roy
authors.forename: Sara J C
authors.initials: SJ
authors.lastname: Gosline
authors.forename: Michael
authors.initials: M
authors.lastname: Hallett
authors.forename: Jacques
authors.initials: J
authors.lastname: Paiement
authors.forename: Robert E
authors.initials: RE
authors.lastname: Kearney
authors.forename: Tommy
authors.initials: T
authors.lastname: Nilsson
authors.forename: John J M
authors.initials: JJ
authors.lastname: Bergeron
mesh_terms: D000818:Animals; D020755:Coat Protein Complex I; D004721:Endoplasmic Reticulum / Q000737:chemistry*; D006056:Golgi Apparatus / Q000737:chemistry*; D008099:Liver / Q000737:chemistry / Q000166:cytology; D021381:Protein Transport; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification*; D040901:Proteomics; D051381:Rats; D050600:SNARE Proteins / Q000302:isolation & purification; D053719:Tandem Mass Spectrometry; D020691:rab GTP-Binding Proteins / Q000235:genetics / Q000302:isolation & purification
chemical_list: D020755:Coat Protein Complex I; D011506:Proteins; D050600:SNARE Proteins; D020691:rab GTP-Binding Proteins