title: [Identification of glial cell line-derived neurotrophic factor recombinant retroviral vector and establishment of its packaging cell line PA317].
abstract: OBJECTIVE
  To identify glial cell line-derived neurotrophic factor (GDNF) recombinant retroviral vector and to establish its packaging cell line PA317.
  
  
  METHODS
  PA317 cells were transfected with recombinant retroviral vector pLXSN-GDNF using liposomes. The recombinant retroviral particles were then harvested from culture media of G418 resistant transfected cells and analyzed using RT-PCR. Virus titers in supernatants were investigated.
  
  
  RESULTS
  Sequencing date indicated that GDNF gene was exactly identical to the sequence in the GeneBank. PA317 cells were transfected with recombinant retroviral vector pLXSN-GDNF using liposomes, and virus titers in supernatants harvested from culture media of (G418 resistant transfected cells were 10(4)10(5) CFU/ml.
  
  
  CONCLUSIONS
  Packaging cell line PA317/pLXSN-GDNF was established.
authors.affiliation: Department of Orthopaedics, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, P. R. China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Zhang
authors.forename: Bingcang
authors.initials: B
authors.lastname: Li
authors.forename: Xianjun
authors.initials: X
authors.lastname: Ren
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D042003:DNA Packaging; D005822:Genetic Vectors; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000235:genetics*; D051379:Mice; D041681:NIH 3T3 Cells; D011994:Recombinant Proteins; D012190:Retroviridae / Q000235:genetics*; D014162:Transfection
chemical_list: D051100:Glial Cell Line-Derived Neurotrophic Factor; D011994:Recombinant Proteins