Characterization of biosurfactant-containing liposomes and their efficiency for gene transfection.
Recently we showed significance of biosurfactants in the field of non-viral vectors for gene transfection. There, a biosurfactant, mannosylerythritol lipid A (MEL-A), especially increased the efficiency of gene transfection mediated with cationic liposomes. However, the molecular mechanism has not been well-understood yet. Here, through the examination of the ability of cationic liposomes containing an MEL (MEL-A, MEL-B or MEL-C) for important transfectional processes of the DNA capsulation and the membrane fusion with anionic liposomes, we found that MEL-A-containing liposomes increased both processes, but that MEL-B and MEL-C-containing liposomes just increased either of them. The results indicated that these kinds of the physicochemical properties in MEL-A-containing liposomes are able to increase the efficiency of liposome-mediated gene transfection.
Ueno
Yoshinobu
Y
Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, Japan.
Hirashima
Naohide
N
Inoh
Yoshikazu
Y
Furuno
Tadahide
T
Nakanishi
Mamoru
M
D000838:Anions; D002412:Cations; D004247:DNA / Q000737:chemistry*; D004996:Ethidium / Q000737:chemistry; D006017:Glycolipids / Q000737:chemistry* / Q000494:pharmacology; D007364:Intercalating Agents / Q000737:chemistry; D008081:Liposomes; D008561:Membrane Fusion / Q000187:drug effects; D013501:Surface-Active Agents / Q000737:chemistry* / Q000494:pharmacology; D014162:Transfection / Q000379:methods*
D000838:Anions; D002412:Cations; D006017:Glycolipids; D007364:Intercalating Agents; D008081:Liposomes; D013501:Surface-Active Agents; C439671:mannosylerythritol lipid; D004247:DNA; D004996:Ethidium