title: [Effects of emodin on proliferation cycle and apoptotic gene of human lung adenocarcinoma cell line Anip 973].
abstract: OBJECTIVE
  To study the suppressive role of emodin on the growth and its effect on the proliferation cycle and apoptotic gene of human lung adenocarcinoma cell line Anip 973.
  
  
  METHODS
  The survival rate and the inhibitory rate of Anip 973 cell in vitro were detected by MTT colorimetric assay and cell growth curve assay at different time points under different concentration of emodin; the cell proliferation cycle and the apoptotic rate were examined with flow cytometry analysis, and Caspase-3 protein expression was measured by immunoblotting assay.
  
  
  RESULTS
  Emodin inhibited the proliferation of Anip 973 cell at G0/G1 phase, decreased the cell ratio at S phase and activated the Caspase-3 protein. It suppressed the growth of tumor cells and raised the apoptotic rate in a concentration and time depending manner in a certain extent.
  
  
  CONCLUSIONS
  Emodin could suppress the proliferation of Anip 973 cell, and its mechanism of anticancer effect may be through activating Caspase-3, to induce apoptosis and block cell cycle.
authors.affiliation: Department of Respiratory Disease, The Second Affiliated Hospital of Harbin Medical University, Harbin. ljn1209475@sina.com.cn
authors.forename: Jia-ning
authors.initials: JN
authors.lastname: Li
authors.forename: Fu-zhen
authors.initials: FZ
authors.lastname: Lv
authors.forename: Jin-ling
authors.initials: JL
authors.lastname: Xiao
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology; D000368:Aged; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004642:Emodin / Q000494:pharmacology*; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology; D008297:Male
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D053148:Caspase 3; D004642:Emodin