Efficient adsorption of tumor necrosis factor with an in vitro set-up of the microspheres-based detoxification system.
BACKGROUND
The Microsperes-Based Detoxification System (MDS) represents a flexible therapeutic option for various diseases, depending on the specificity of the adsorbents applied. A potential application of the MDS is the supportive therapy of sepsis.


METHODS
Microadsorbents for tumor necrosis factor (TNF) were prepared by immobilization of anti-TNF antibodies on cellulose (1-10 microm) and applied in an experimental set-up using a pool of human plasma (1 liter) spiked with TNF (800 pg/ml) and its soluble receptors (1,000 pg/ml each). Removal of TNF was compared using a plasma filter and the albumin-permeable filter Albuflow.


RESULTS
Addition of 4 (2) g of adsorbent to the filtrate circuit reduced TNF concentrations in the pool by 80% (64%). Removal rates did not differ significantly for the different filters. TNF adsorption was not influenced by the presence of excess TNF receptors.


CONCLUSIONS
Concentrations of mediators can be efficiently modulated with the MDS using small quantities of specific adsorbents.
Weber
Viktoria
V
Center for Biomedical Technology, Danube University Krems, Krems, Austria. viktoria.weber@donau-uni.ac.at
Hartmann
Jens
J
Linsberger
Ingrid
I
Falkenhagen
Dieter
D
D000327:Adsorption; D006464:Hemoperfusion / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D008863:Microspheres; D014409:Tumor Necrosis Factor-alpha / Q000302:isolation & purification*
D014409:Tumor Necrosis Factor-alpha