The repairing effect of a recombinant human connective-tissue growth factor in a burn-wounded rhesus-monkey (Macaca mulatta) model.
CTGF (connective-tissue growth factor) has been characterized as an extracellular-matrix-associated protein that modulates basic-fibroblast-growth-factor signalling and angiogenesis. In the present paper, the cloning of the ctgf gene from human umbilical-vein endothelial cells and expression of the protein in Escherichia coli as an N-terminal hexahistidine fusion protein is described. Recombinant human CTGF (rhCTGF) was expressed and purified so that we could investigate its effect on the proliferation of human embryo fibroblast KMB-17 and NIH3T3 cells. The results indicated not only that the protein was properly folded, but also that it had the same specific activity and stability as the native protein. Furthermore, we administered this recombinant protein in a non-human primate [rhesus monkey (Macaca mulatta)] burn-wound model and report the clinical findings and structural effects. Epitheliotrophic effects were conspicuous in wounded tissues at 10-100 ng of CTGF/cm(2), suggesting that administered rhCTGF can play a normal physiological role in wound repairing in a non-human primate model.
Liu
Long-ding
LD
Institute of Medical Biology, Chinese Academy of Medical Sciences, Peking Union Medical College, Kunming, People's Republic of China.
Shi
Hai-jing
HJ
Jiang
Li
L
Wang
Li-chun
LC
Ma
Shao-hui
SH
Dong
Cheng-hong
CH
Wang
Jing-jing
JJ
Zhao
Hong-ling
HL
Liao
Yun
Y
Li
Qi-han
QH
D000818:Animals; D002056:Burns / Q000188:drug therapy* / Q000473:pathology*; D002460:Cell Line; D055513:Connective Tissue Growth Factor; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D006801:Humans; D017874:Immediate-Early Proteins / Q000008:administration & dosage* / Q000235:genetics; D036341:Intercellular Signaling Peptides and Proteins / Q000008:administration & dosage* / Q000235:genetics; D051379:Mice; D041681:NIH 3T3 Cells; D011994:Recombinant Proteins / Q000008:administration & dosage; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects*
C526317:CCN2 protein, human; C526318:CCN2 protein, mouse; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D011994:Recombinant Proteins; D055513:Connective Tissue Growth Factor