title: Testing and evaluation of 43 "noncore" Y chromosome markers for forensic casework applications.
abstract: A developmental validation study was performed on three Y-STR multiplex systems, Multiplex III (MPIII), Multiplex IV (MPIV), and Multiplex V (MPV), to ascertain their potential applicability to forensic casework. MPIII contains eight Y-STRs, including DYS426, DYS435, DYS436, DYS441, DYS442, DYS446, DYS462, and Y-GATA-A10, and one InDel, YAP (DYS287). MPIV contains 21 Y-STR loci, including DYS443, DYS444, DYS445, DYS447, DYS448, DYS449, DYS452, DYS453, DYS454, DYS455, DYS456, DYS458, DYS463, DYS464, DYS468, DYS484, DYS522, DYS527, DYS531 DYS557, and DYS588. MPV contains 13 Y-STR loci, including DYS459, DYS476, DYS488, DYS513, DYS549, DYS561, DYS570, DYS575, DYS576, DYS590, DYS594, DYS598, and DYS607. Full genetic profiles were consistently obtained for all three multiplexes with 25-50 pg of male DNA. No significant amplification was observed with 1 mug of female DNA. Each multiplex permitted the determination of the number of male donors in male:male DNA admixtures. Species specificity studies demonstrated some cross-reactivity with some primate samples. Environmentally compromised blood samples produced full or partial profiles after exposure to various conditions for up to 1 year. Full profiles were recovered from simulated casework specimens including cigarette butts and postcoital cervicovaginal swabs. Population data were collected to determine individual loci gene diversity and multiplex discriminatory capacity.
authors.affiliation: Graduate Program in Biomolecular Science, University of Central Florida, PO Box, 162366, Orlando, FL 32816-2366, USA.
authors.forename: Erin K
authors.initials: EK
authors.lastname: Hanson
authors.forename: Paulina N
authors.initials: PN
authors.lastname: Berdos
authors.forename: Jack
authors.initials: J
authors.lastname: Ballantyne
mesh_terms: D000818:Animals; D001769:Blood; D041322:Chromosomes, Human, Y; D004247:DNA / Q000302:isolation & purification; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D053803:Forensic Genetics; D005819:Genetic Markers; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D013094:Spermatozoa; D020080:Tandem Repeat Sequences
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D005819:Genetic Markers; D004247:DNA