SPECT imaging, immunohistochemical and behavioural correlations in the primate models of Parkinson's disease.
Dopamine active transporter (DAT) single photon emission computerised tomography (SPECT) is considered a useful and practical technique for early diagnosis of Parkinson's disease (PD) and assessment of its progression. The application of this technique, particularly as a surrogate marker for therapeutic and neuroprotective trials in Parkinsonism, however, is dependent on pathological validation. In the absence of human studies, we used 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) primate models of Parkinsonism to verify correlation between the SPECT, immunohistochemical and behavioural data. The DAT SPECT data correlated strongly and significantly with the substantia nigra pars compacta tyrosine hydroxylase and Nissl cell counts as well as the behavioural scores. Within the limitations of small numbers inherent to such studies, this data provides the first attempt at pathological validation of SPECT in primates.
Ashkan
Keyoumars
K
Department of Clinical Neurosciences, University Joseph Fourier of Grenoble, France. K.Ashkan@ion.ucl.ac.uk
Wallace
Bradley A
BA
Mitrofanis
John
J
Pollo
Claudio
C
Brard
Pierre-Yves
PY
Fagret
Daniel
D
Benabid
Alim-Louis
AL
D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D000704:Analysis of Variance; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology*; D004195:Disease Models, Animal; D050483:Dopamine Plasma Membrane Transport Proteins / Q000378:metabolism; D007839:Functional Laterality; D008252:Macaca fascicularis; D008297:Male; D009474:Neurons / Q000378:metabolism; D020734:Parkinsonian Disorders / Q000139:chemically induced / Q000000981:diagnostic imaging* / Q000378:metabolism* / Q000503:physiopathology; D012720:Severity of Illness Index; D013378:Substantia Nigra / Q000473:pathology; D013997:Time Factors; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D050483:Dopamine Plasma Membrane Transport Proteins; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D014446:Tyrosine 3-Monooxygenase