title: A fast and reliable single-run method for genotyping of the human cytochrome P450 2C8 gene for different ethnic groups.
abstract: Patients vary widely in their response to drug therapy according to their genetic background of drug metabolizing enzymes. The cytochrome P450 enzyme CYP2C8 is one of the major metabolizing enzymes involved in drug metabolism and thus a candidate for routine pharmacogenetic screening. The aim of this work was establishing a fast and reliable method to detect the three CYP2C8 genotypes CYP2C8*2, CYP2C8*3, CYP2C8*4, and the wildtype allele. An established real-time polymerase chain reaction (PCR) to detect two CYP2C8 genotypes was extended by introduction of a third hybridization probe. After optimization of running conditions, the new triplex method was evaluated using 200 DNAs of African origin as templates. Standard methods were performed as controls. The new triplex real-time PCR was fast, reliable and reproducible. The obtained results showed no deviation from the results of the established technique. The polymorphism of the CYP2C8 gene among an African population showed the expected distribution (68% wildtype gene, 32% at least one CYP2C8*2 allele). Pharmacogenetics gain increasing interest in routine medical care to prevent severe adverse effects or the application of ineffective drugs. We here provide a fast, reliable and reproducible method in one single assay run to detect three relevant CYP2C8 alleles independent of the patient's ethnic origin.
authors.affiliation: Institute for Clinical Research and Development (IKFE), Mainz, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Weise
authors.forename: U
authors.initials: U
authors.lastname: Lambertz
authors.forename: N
authors.initials: N
authors.lastname: Thomé
authors.forename: U
authors.initials: U
authors.lastname: Bartz
authors.forename: M
authors.initials: M
authors.lastname: Krefft
authors.forename: F
authors.initials: F
authors.lastname: Mockenhaupt
authors.forename: U
authors.initials: U
authors.lastname: Bienzle
authors.forename: T
authors.initials: T
authors.lastname: Schöndorf
authors.forename: T
authors.initials: T
authors.lastname: Forst
authors.forename: A
authors.initials: A
authors.lastname: Pfützner
mesh_terms: D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics*; D044383:Black People / Q000235:genetics*; D065727:Cytochrome P-450 CYP2C8; D005838:Genotype; D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D044469:Racial Groups / Q000235:genetics*
chemical_list: D001189:Aryl Hydrocarbon Hydroxylases; C450259:CYP2C8 protein, human; D065727:Cytochrome P-450 CYP2C8