Inhibition of angiogenic differentiation of human umbilical vein endothelial cells by diallyl disulfide (DADS).
Angiogenesis is a crucial step in the growth and metastasis of cancers. The activation of endothelial cells and their further behaviour are very critical during angiogenesis. We analyzed the effect of diallyl disulfide (DADS) on angiogenesis in in vitro models using human umbilical vein endothelial cells (HUVECs). DADS significantly inhibited endothelial cell migration, invasion and tube formation. (3)H-thymidine proliferation assay clearly showed the inhibitory effect of DADS on the proliferation of HUVECs in vitro. The role of metalloproteinases has been shown to be important in angiogenesis; therefore, zymography was performed to determine whether DADS affected protease activity. Gelatin zymographic analysis showed the inhibitory effect of DADS on the activation of matrix metalloproteinases-MMP-2 and MMP-9. These findings suggest that DADS acts as an angiogenesis inhibitor by inhibiting the activation of matrix metalloproteinases during endothelial morphogenesis.
Thejass
P
P
Amala Cancer Research Centre, Amala Nagar, Thrissur, Kerala State 680555, India.
Kuttan
Girija
G
D000498:Allyl Compounds / Q000494:pharmacology*; D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004220:Disulfides / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects* / Q000473:pathology; D006801:Humans; D008954:Models, Biological; D009389:Neovascularization, Pathologic / Q000517:prevention & control*; D014471:Umbilical Veins / Q000166:cytology
D000498:Allyl Compounds; D020533:Angiogenesis Inhibitors; D004220:Disulfides; C028009:diallyl disulfide