title: High glucose promotes the release and expression of novel vasoactive peptide, coupling factor 6, in human umbilical vein endothelial cells.
abstract: As a novel vasoactive peptide, plasma coupling factor 6 (CF6) was shown to be elevated in patients with diabetes mellitus, yet the mechanism involved is unknown. We studied CF6 protein release and its potential mechanism in human umbilical vein endothelial cells (HUVECs) incubated with high glucose levels. High glucose level enhanced CF6 expression and peptide secretion in HUVECs in a time- and concentration-dependent manner, which was independent of increased osmolarity. PKC or p38 MAPK inhibition significantly suppressed high glucose-mediated CF6 release in HUVECs, and the inhibition rate was -45% and -30%, respectively. Also, high glucose-induced CF6 production was antagonized by insulin treatment. Hence, high glucose increases the expression and secretion of CF6 in endothelial cells and appears to be mediated by PKC and p38 MAPK activity.
authors.affiliation: Shandong University School of Medicine, Ji'nan 250012, China.
authors.forename: Xiaolu
authors.initials: X
authors.lastname: Li
authors.forename: Shanshan
authors.initials: S
authors.lastname: Xing
authors.forename: Like
authors.initials: L
authors.lastname: Zhang
authors.forename: Qichong
authors.initials: Q
authors.lastname: Xing
authors.forename: Suhua
authors.initials: S
authors.lastname: Yan
authors.forename: Hongyan
authors.initials: H
authors.lastname: Dai
authors.forename: Shuling
authors.initials: S
authors.lastname: You
authors.forename: Yongzheng
authors.initials: Y
authors.lastname: Pang
authors.forename: Chaoshu
authors.initials: C
authors.lastname: Tang
mesh_terms: D001483:Base Sequence; D002478:Cells, Cultured; D017931:DNA Primers / Q000235:genetics; D003920:Diabetes Mellitus / Q000235:genetics / Q000378:metabolism; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects; D025261:Mitochondrial Proton-Translocating ATPases / Q000096:biosynthesis* / Q000235:genetics*; D010086:Oxidative Phosphorylation Coupling Factors / Q000096:biosynthesis* / Q000235:genetics*; D011493:Protein Kinase C / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D017931:DNA Primers; D010086:Oxidative Phosphorylation Coupling Factors; D012333:RNA, Messenger; D011493:Protein Kinase C; C000628217:F(6) ATPase; D025261:Mitochondrial Proton-Translocating ATPases; D005947:Glucose