title: The twin-arginine translocation pathway is necessary for correct membrane insertion of the Rieske Fe/S protein in Legionella pneumophila.
abstract: The twin-arginine translocation (Tat) pathway translocates folded proteins across the cytoplasmic membrane. Proteins transported through this secretion system typically carry two arginine residues in their signal peptide that is cleaved off during translocation. Recently, we demonstrated the presence of the Tat pathway in Legionella pneumophila Philadelphia-1 and the Rieske Fe/S protein PetA was one of the predicted Tat substrates. Because we observed that the signal peptide of PetA is not processed and that this protein is still membrane associated in the tat mutants, correct membrane insertion was assayed using a trypsin sensitivity assay. We conclude that the Tat pathway is necessary for correct membrane insertion of L. pneumophila PetA.
authors.affiliation: Laboratory of Bacteriology, Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium.
authors.forename: Emmy
authors.initials: E
authors.lastname: De Buck
authors.forename: Leen
authors.initials: L
authors.lastname: Vranckx
authors.forename: Eef
authors.initials: E
authors.lastname: Meyen
authors.forename: Liesbeth
authors.initials: L
authors.lastname: Maes
authors.forename: Liesbeth
authors.initials: L
authors.lastname: Vandersmissen
authors.forename: Jozef
authors.initials: J
authors.lastname: Anné
authors.forename: Elke
authors.initials: E
authors.lastname: Lammertyn
mesh_terms: D001120:Arginine / Q000378:metabolism; D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D014450:Electron Transport Complex III / Q000032:analysis / Q000378:metabolism*; D007506:Iron-Sulfur Proteins / Q000032:analysis / Q000378:metabolism*; D016952:Legionella pneumophila / Q000235:genetics / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D021381:Protein Transport
chemical_list: D001426:Bacterial Proteins; D007506:Iron-Sulfur Proteins; D026901:Membrane Transport Proteins; C037989:Rieske iron-sulfur protein; D001120:Arginine; D014450:Electron Transport Complex III