Comparative study of cryopreserved bone tissue and tissue preserved in a 98% glycerol solution.
OBJECTIVE
To compare the bone graft cryopreservation method (at -80 degrees C) with a preservation method using a 98% glycerol solution at room temperature (10 degrees C-35 degrees C), by testing the antibacterial and fungal effects of 98% glycerol and comparatively analyzing the observed histological changes resulting from the use of both methods.


METHODS
This study was of 30 samples of trabecular bone tissue from 10 patients undergoing total hip arthroplasty. Each femoral head provided 3 samples that were randomized into 3 groups, namely, the control group, the cryopreserved group, and the group preserved in a 98% glycerol at room temperature for 1 year. The samples were submitted to histomorphologic, cell feasibility, and microbiologic analyses. The results were statistically analyzed using the McNemar test, with a statistical significance index of 0.05.


RESULTS
Values obtained using the McNemar test to compare probability distributions of histomorphologic variables (mature or lamellar bone, immature bone, and necrosis) and cell feasibility (osteoblasts and osteoclasts) indicated that there is no difference between the distributions of variables under the 3 experimental conditions. Microbiological analysis of the 98% glycerol solution and bone fragments from samples stored for 1 year at room temperature did not show bacterial or fungal growth. The histological and microbiological investigation were performed at 2 different time points: immediately after the sample processing and after 1 year.


CONCLUSIONS
The method used to preserve bone grafts kept in 98% glycerol at room temperature (10 degrees C-35 degrees C) was similar to cryopreservation in terms of bone matrix preservation; no bacteria or fungi were found in the samples.
Giovani
Arlete Mazzini Miranda
AM
Tissue Bank, Institute of Orthopedics and Traumatology, Hospital das Clínicas, São Paulo University Medical School, São Paulo, SP, Brazil. giovani@inin.com.br
Croci
Alberto Tesconi
AT
Oliveira
Cláudia Regina G C M
CR
Filippi
Renée Zon
RZ
Santos
Luiz Augusto U
LA
Maragni
Graziela G
GG
Albhy
Thays Moreira
TM
D001857:Bone Matrix / Q000187:drug effects / Q000382:microbiology; D001842:Bone and Bones / Q000187:drug effects* / Q000382:microbiology; D015925:Cryopreservation / Q000592:standards*; D003451:Cryoprotective Agents / Q000737:chemistry / Q000494:pharmacology*; D005990:Glycerol / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D016555:Matched-Pair Analysis; D015233:Models, Statistical; D013696:Temperature; D014021:Tissue Preservation / Q000379:methods / Q000592:standards*
D003451:Cryoprotective Agents; D005990:Glycerol