Guidelines for the selection of effective short-interfering RNA sequences for functional genomics.
To avoid long-double-stranded-RNA-dependent interferon response, short-interfering RNAs (siRNAs) are widely used for RNA interference (RNAi) in mammalian cells. siRNA-based RNAi, however, may not be readily available for the large-scale gene silencing essential for systematic functional genomics, because only a limited fraction of siRNAs is capable of inducing effective mammalian RNAi. siRNAs correctly designed for the knockdown of a particular gene may also destroy the functions of unrelated genes. Here, we describe algorithms by which these serious setbacks can be eliminated in mammalian functional genomics using RNAi and a Web-based online software system for computing highly functional siRNA sequences with maximal target-specificity in mammalian RNAi.
Ui-Tei
Kumiko
K
Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Tokyo, Japan.
Naito
Yuki
Y
Saigo
Kaoru
K
D000465:Algorithms; D000818:Animals; D002460:Cell Line; D023281:Genomics / Q000379:methods; D017408:Guidelines as Topic; D006801:Humans; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics*; D012984:Software
D012330:RNA, Double-Stranded; D034741:RNA, Small Interfering