title: Phospholipase Cgamma1 stimulates transcriptional activation of the matrix metalloproteinase-3 gene via the protein kinase C/Raf/ERK cascade.
abstract: The phospholipid hydrolase phospholipase Cgamma1 (PLCgamma1) plays a major role in regulation of cell proliferation, development, and cell motility. Overexpression of PLCgamma1 is associated with tumor development, and it is overexpressed in some tumors. Matrix metalloproteinase-3 (MMP-3) is a protein involved in tumor invasion and metastasis. Here, we demonstrate that overexpression of PLCgamma1 stimulates MMP-3 expression at the transcriptional level via the PKC-mediated Raf/MEK1/ERK signaling cascade. We propose that modulation of PLCgamma1 activity might be of value in controlling the activity of MMPs, which are important regulators of invasion and metastasis in malignant tumors.
authors.affiliation: Institute of Natural Science and Technology, Hanyang University, Ansan 426-791, South Korea.
authors.forename: Soon Young
authors.initials: SY
authors.lastname: Shin
authors.forename: Ha Young
authors.initials: HY
authors.lastname: Choi
authors.forename: Bong-Hyun
authors.initials: BH
authors.lastname: Ahn
authors.forename: Do Sik
authors.initials: do S
authors.lastname: Min
authors.forename: Sang Wook
authors.initials: SW
authors.lastname: Son
authors.forename: Young Han
authors.initials: YH
authors.lastname: Lee
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology; D002478:Cells, Cultured; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D019278:Matrix Metalloproteinase 3 / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D041681:NIH 3T3 Cells; D051966:Phospholipase C gamma / Q000502:physiology*; D011401:Promoter Regions, Genetic / Q000187:drug effects; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D015398:Signal Transduction / Q000502:physiology*; D014945:Wound Healing / Q000502:physiology; D048490:raf Kinases / Q000378:metabolism*
chemical_list: D048490:raf Kinases; D011493:Protein Kinase C; D048049:Extracellular Signal-Regulated MAP Kinases; D051966:Phospholipase C gamma; D019278:Matrix Metalloproteinase 3