Comparison of pancreas juice proteins from cancer versus pancreatitis using quantitative proteomic analysis.
OBJECTIVE
Pancreatitis is an inflammatory condition of the pancreas. However, it often shares many molecular features with pancreatic cancer. Biomarkers present in pancreatic cancer frequently occur in the setting of pancreatitis. The efforts to develop diagnostic biomarkers for pancreatic cancer have thus been complicated by the false-positive involvement of pancreatitis.


METHODS
In an attempt to develop protein biomarkers for pancreatic cancer, we previously use quantitative proteomics to identify and quantify the proteins from pancreatic cancer juice. Pancreatic juice is a rich source of proteins that are shed by the pancreatic ductal cells. In this study, we used a similar approach to identify and quantify proteins from pancreatitis juice.


RESULTS
In total, 72 proteins were identified and quantified in the comparison of pancreatic juice from pancreatitis patients versus pooled normal control juice. Nineteen of the juice proteins were overexpressed, and 8 were underexpressed in pancreatitis juice by at least 2-fold compared with normal pancreatic juice. Of these 27 differentially expressed proteins in pancreatitis, 9 proteins were also differentially expressed in the pancreatic juice from pancreatic cancer patient.


CONCLUSIONS
Identification of these differentially expressed proteins from pancreatitis juice provides useful information for future study of specific pancreatitis-associated proteins and to eliminate potential false-positive biomarkers for pancreatic cancer.
Chen
Ru
R
Division of Gastroenterology/Department of Medicine, University of Washington, Seattle, WA, USA.
Pan
Sheng
S
Cooke
Kelly
K
Moyes
Kara White
KW
Bronner
Mary P
MP
Goodlett
David R
DR
Aebersold
Ruedi
R
Brentnall
Teresa A
TA
D014408:Biomarkers, Tumor / Q000032:analysis / Q000378:metabolism*; D004798:Enzymes / Q000032:analysis / Q000378:metabolism; D005340:Fibrinogen / Q000032:analysis / Q000378:metabolism; D006801:Humans; D019006:Neural Cell Adhesion Molecules / Q000032:analysis / Q000378:metabolism; D010189:Pancreatic Juice / Q000378:metabolism*; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D010195:Pancreatitis / Q000378:metabolism*; D010958:Plasminogen / Q000032:analysis / Q000378:metabolism; D040901:Proteomics; D012697:Serine Endopeptidases / Q000032:analysis / Q000378:metabolism
D014408:Biomarkers, Tumor; D004798:Enzymes; D019006:Neural Cell Adhesion Molecules; D005340:Fibrinogen; D010958:Plasminogen; D012697:Serine Endopeptidases; C074385:caldecrin