Gene trap mutagenesis.
Our ability to genetically manipulate the mouse has had a great impact on medical research over the last few decades. Mouse genetics has developed into a powerful tool for dissecting the genetic causes of human disease and identifying potential targets for pharmaceutical intervention. With the recent sequencing of the human and mouse genomes, a large number of novel genes have been identified whose function in normal and disease physiology remains largely unknown. Government-sponsored multinational efforts are underway to analyze the function of all mouse genes through mutagenesis and phenotyping, making the mouse the interpreter of the human genome. A number of technologies are available for the generation of mutant mice, including gene targeting, gene trapping and transposon, chemical or radiation-induced mutagenesis. In this chapter, we review the current status of gene trapping technology, including its applicability to conditional mutagenesis.
Abuin
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Lexicon Genetics, 8800 Technology Forest Place, The Woodlands, TX 77381, USA. aabuin@lexgen.com
Hansen
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Zambrowicz
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B
D000483:Alleles; D000818:Animals; D018390:Gene Targeting / Q000379:methods*; D005838:Genotype; D006801:Humans; D016296:Mutagenesis / Q000235:genetics*; D009154:Mutation / Q000235:genetics