title: Mapping the 5' ends of banana bunchy top virus gene transcripts.
abstract: Banana bunchy top virus (BBTV), a multi-component circular ssDNA virus, replicates via a dsDNA intermediate that also serves as a template for virion sense transcription. Seven virus-derived transcripts have been previously identified and analysed in BBTV-infected bananas by northern analysis and 3' rapid amplification of cDNA ends (3' RACE). In this study, we have used RNA ligase-mediated rapid amplification of 5' cDNA ends (RLM-RACE) to complete the mapping of the BBTV gene transcripts and have now fully mapped the transcribed regions of each BBTV component and effectively defined the upstream regulatory region.
authors.affiliation: Centre for Tropical Crops and Biocommodities, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia.
authors.forename: V A
authors.initials: VA
authors.lastname: Herrera-Valencia
authors.forename: B
authors.initials: B
authors.lastname: Dugdale
authors.forename: R M
authors.initials: RM
authors.lastname: Harding
authors.forename: J L
authors.initials: JL
authors.lastname: Dale
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics*; D052658:Babuvirus / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D004279:DNA, Viral / Q000235:genetics*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D014158:Transcription, Genetic
chemical_list: D020121:5' Untranslated Regions; D017931:DNA Primers; D004279:DNA, Viral