Transcriptome of local innate and adaptive immunity during early phase of infectious bronchitis viral infection.
To understand the mechanistic basis of local innate and adaptive immunity against infectious bronchitis virus (IBV) at the molecular level, we examined the gene transcription profile of tracheal epithelial layers 3 d after infection of chickens with an attenuated IBV-Massachusetts strain. Results suggested that the transcription levels of 365 genes were either upregulated or downregulated (2-fold and higher) after IBV infection. Among the upregulated 250 genes, 25 were directly immune-related genes. These upregulated immune response genes included TLR2, TLR3, interferon-induced antiviral genes (Mx), and genes responsible for cytotoxic T cell killing such as Fas antigen and granzyme-A. Overall, a diversity of innate immunity and helper T cell type 1 (Th1)-biased adaptive immunity are activated in the host's early defense against IBV invasion, and they are responsible for the rapid clearance of virus from the local infection.
Wang
Xiuqing
X
Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57007, USA. xiuqing.wang@sdstate.edu
Rosa
Artur J M
AJ
Oliverira
Henrique N
HN
Rosa
Guilherme J M
GJ
Guo
Xueshui
X
Travnicek
Mark
M
Girshick
Theodore
T
D000818:Animals; D002645:Chickens; D018352:Coronavirus Infections / Q000276:immunology*; D004848:Epithelium / Q000276:immunology / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D053804:Granzymes / Q000235:genetics / Q000378:metabolism; D007110:Immunity, Active; D007113:Immunity, Innate; D001351:Infectious bronchitis virus; D007372:Interferons / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D014132:Trachea / Q000276:immunology; D019014:fas Receptor / Q000235:genetics / Q000378:metabolism
D019014:fas Receptor; D007372:Interferons; D053804:Granzymes