title: Laminins containing the beta2 chain modulate the precise organization of CNS synapses.
abstract: Synapses are formed and stabilized by concerted interactions of pre-, intra-, and post-synaptic components; however, the precise nature of the intrasynaptic components in the CNS remains obscure. Potential intrasynaptic components include extracellular matrix molecules such as laminins; here, we isolate beta2-containing laminins, including perhaps laminins 13 (alpha3beta2gamma3) and 14 (alpha4beta2gamma3), from CNS synaptosomes suggesting a role for these molecules in synaptic organization. Indeed, hippocampal synapses that form in vivo in the absence of these laminins are malformed at the ultrastructural level and this malformation is replicated in synapses formed in vitro, where laminins are provided largely by the post-synaptic neuron. This recapitulation of the in vivo function of laminins in vitro suggests that the malformations are a direct consequence of the removal of laminins from the synapse. Together, these results support a role for neuronal laminins in the structural integrity of central synapses.
authors.affiliation: Department of Neuroscience, Tufts Center for Vision Research, Boston, MA 02111, USA.
authors.forename: Christophe
authors.initials: C
authors.lastname: Egles
authors.forename: Thomas
authors.initials: T
authors.lastname: Claudepierre
authors.forename: Mary K
authors.initials: MK
authors.lastname: Manglapus
authors.forename: Marie-France
authors.initials: MF
authors.lastname: Champliaud
authors.forename: William J
authors.initials: WJ
authors.lastname: Brunken
authors.forename: Dale D
authors.initials: DD
authors.lastname: Hunter
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology*; D007797:Laminin / Q000172:deficiency / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D046529:Microscopy, Electron, Transmission / Q000379:methods; D008954:Models, Biological; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D013569:Synapses / Q000502:physiology* / Q000648:ultrastructure; D016704:Synapsins / Q000378:metabolism; D013574:Synaptosomes / Q000737:chemistry / Q000378:metabolism
chemical_list: D007797:Laminin; D016704:Synapsins; C058783:laminin beta2