title: Mutations of acetylcholinesterase1 contribute to prothiofos-resistance in Plutella xylostella (L.).
abstract: Insensitive acetylcholinesterase (AChE) is involved in the resistance of organophosphorous and carbamate insecticides. We cloned a novel full-length AChE cDNA encoding ace1 gene from adult heads of the diamondback moth (DBM, Plutella xylostella). The ace1 gene encoding 679 amino acids has conserved motifs including catalytic triad, choline-binding site and acyl pocket. Northern blot analysis revealed that the ace1 gene was expressed much higher than the ace2 in all examined body parts. The biochemical properties of expressed AChEs showed substrate specificity for acetylthiocholine iodide and inhibitor specificity for BW284C51 and eserine. Three mutations of AChE1 (D229G, A298S, and G324A) were identified in the prothiofos-resistant strain, two of which (A298S and G324A) were expected to be involved in the prothiofos-resistance through three-dimensional modeling. In vitro functional expression of AChEs in Sf9 cells revealed that only resistant AChE1 is less inhibited with paraoxon, suggesting that resistant AChE1 is responsible for prothiofos-resistance.
authors.affiliation: Ilsong Institute of Life Science, Hallym University, Anyang 431-060, Republic of Korea.
authors.forename: Dae-Weon
authors.initials: DW
authors.lastname: Lee
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Choi
authors.forename: Won Tae
authors.initials: WT
authors.lastname: Kim
authors.forename: Yeon Ho
authors.initials: YH
authors.lastname: Je
authors.forename: Jong Tae
authors.initials: JT
authors.lastname: Song
authors.forename: Bu Keun
authors.initials: BK
authors.lastname: Chung
authors.forename: Kyung Saeng
authors.initials: KS
authors.lastname: Boo
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Koh
mesh_terms: D000110:Acetylcholinesterase / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D002093:Benzenaminium, 4,4'-(3-oxo-1,5-pentanediyl)bis(N,N-dimethyl-N-2-propenyl-), Dibromide / Q000494:pharmacology; D002800:Cholinesterase Inhibitors; D007305:Insecticide Resistance / Q000235:genetics*; D008969:Molecular Sequence Data; D009036:Moths / Q000187:drug effects / Q000235:genetics*; D063086:Organothiophosphates / Q000494:pharmacology*; D010261:Paraoxon / Q000494:pharmacology; D010640:Phenothiazines / Q000494:pharmacology; D010830:Physostigmine / Q000494:pharmacology; D013379:Substrate Specificity
chemical_list: D002800:Cholinesterase Inhibitors; D063086:Organothiophosphates; D010640:Phenothiazines; D002093:Benzenaminium, 4,4'-(3-oxo-1,5-pentanediyl)bis(N,N-dimethyl-N-2-propenyl-), Dibromide; C084820:profenamine; D010830:Physostigmine; D000110:Acetylcholinesterase; C032072:prothiophos; D010261:Paraoxon