title: Molecular cloning of bovine FABGL gene and its effects on bovine bioeconomic traits.
abstract: The complete CDS sequence of the bovine FABGL gene was determined by homology cloning approach combined with RT-PCR and 3'- and 5'-RACE. The results of sequence analysis and bioinformatics study showed that this cDNA contained 994 nucleotides, with a 780 bp open reading frame (ORF) flanked by a 16 bp 5'-UTR (incompletely) and a 198 bp 3'-UTR. The deduced amino acid sequence (260 AA) shows 88% identity with the corresponding sequence in humans. Two single nucleotide substitutions, one located in intron 5 (I5) at position 1 065 bp (Y = C/T) (GenBank: DQ409814) and the other in intron 8 (I8) at position 1 792 bp (R = A/G), were detected using the PCR-SSCP method. Analysis of the allele frequencies of the two polymorphic sites in three different cattle breeds (Angus, Hereford, and Simmental) with different genotypes showed large differences: in locus I8, cattle with the GG genotype showed higher beef performance index (BPI) (4.283 +/- 0.475 kg/cm) in comparison with cattle with the AA genotype (4.008 +/- 0.465 kg/cm) (P = 0.01). Regarding the ribeye area, cattle with the GG genotype showed significantly higher ribeye area (73.380 +/- 13.005 cm(2)) compared with cattle with the AA genotype (67.744 +/- 12.777 cm(2)) (p = 0.05). In locus I5, some associations for the average daily gain (ADG) were found at the significance level of 0.01 between three different genotypes (CC, CT, TT): cattle with the TT genotype showed the highest ADG (0.652 +/- 0.330 kg/d), whereas cattle with the CC genotype showed the lowest ADG value (0.421 +/- 0.178 kg/d).
authors.affiliation: Institue of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100094, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Ma
authors.forename: Shang-Zhong
authors.initials: SZ
authors.lastname: Xu
authors.forename: Xue
authors.initials: X
authors.lastname: Gao
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Ren
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Xin
authors.forename: Shu-Xin
authors.initials: SX
authors.lastname: Gao
authors.forename: Ying-Han
authors.initials: YH
authors.lastname: Zhang
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000033:anatomy & histology* / Q000235:genetics* / Q000254:growth & development; D003001:Cloning, Molecular; D019295:Computational Biology; D004247:DNA / Q000737:chemistry / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D016678:Genome / Q000235:genetics; D005838:Genotype; D006649:Histocompatibility Antigens / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008460:Meat; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide
chemical_list: D005819:Genetic Markers; C079876:H2-Ke6 protein, mouse; D006649:Histocompatibility Antigens; D004247:DNA; D010088:Oxidoreductases