Inhibitory complex of the transmembrane ammonia channel, AmtB, and the cytosolic regulatory protein, GlnK, at 1.96 A.
Ammonia conductance is highly regulated. A P(II) signal transduction protein, GlnK, is the final regulator of transmembrane ammonia conductance by the ammonia channel AmtB in Escherichia coli. The complex formed between AmtB and inhibitory GlnK at 1.96-A resolution shows that the trimeric channel is blocked directly by GlnK and how, in response to intracellular nitrogen status, the ability of GlnK to block the channel is regulated by uridylylation/deuridylylation at Y51. ATP and Mg(2+) augment the interaction of GlnK. The hydrolyzed product, adenosine 5'-diphosphate orients the surface of GlnK for AmtB blockade. 2-Oxoglutarate diminishes AmtB/GlnK association, and sites for 2-oxoglutarate are evaluated.
Gruswitz
Franz
F
Department of Biochemistry and Biophysics, Genentech Hall, School of Medicine, University of California, 600 16th Street, San Francisco, CA 94158-2517, USA.
O'Connell
Joseph
J
Stroud
Robert M
RM
D000255:Adenosine Triphosphate / Q000378:metabolism; D027682:Cation Transport Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000502:physiology; D007656:Ketoglutaric Acids / Q000378:metabolism; D009713:Nucleotidyltransferases / Q000737:chemistry* / Q000502:physiology; D051922:PII Nitrogen Regulatory Proteins / Q000737:chemistry* / Q000502:physiology; D019897:Periplasm / Q000378:metabolism; D011487:Protein Conformation; D011499:Protein Processing, Post-Translational; D000644:Quaternary Ammonium Compounds / Q000378:metabolism
C111096:AmtB protein, E coli; D027682:Cation Transport Proteins; D029968:Escherichia coli Proteins; D007656:Ketoglutaric Acids; D051922:PII Nitrogen Regulatory Proteins; D000644:Quaternary Ammonium Compounds; D000255:Adenosine Triphosphate; C497878:glnK protein, E coli; D009713:Nucleotidyltransferases