Structural determination of the O-antigenic polysaccharide from Escherichia coli O166.
The O-antigen of the lipopolysaccharide from Escherichia coli O166 has been determined by component analysis together with 1D and 2D NMR spectroscopy techniques. The polysaccharide has pentasaccharide repeating units consisting of D-glucose (1), D-galactose (2) and N-acetyl-D-galactosamine (2) with the following structure: [
SEE TEXT]. In the 1H NMR, spectrum resonances of low intensity were observed. Further analysis of these showed that they originate from the terminal part of the polysaccharide, thereby revealing that the repeating unit has a 3-substituted N-acetyl-D-galactosamine residue at its reducing end.
Ali
Tara
T
Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, and Karolinska Institute, Department of Laboratory Medicine, Division of Clinical Bacteriology, Karolinska University Hospital, S-141 86 Stockholm, Sweden.
Weintraub
Andrej
A
Widmalm
Göran
G
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002849:Chromatography, Gas; D004926:Escherichia coli / Q000737:chemistry* / Q000276:immunology; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D019081:O Antigens / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*
D019081:O Antigens; D011134:Polysaccharides