title: Prediction of structures of multidomain proteins from structures of the individual domains.
abstract: We describe the development of a method for assembling structures of multidomain proteins from structures of isolated domains. The method consists of an initial low-resolution search in which the conformational space of the domain linker is explored using the Rosetta de novo structure prediction method, followed by a high-resolution search in which all atoms are treated explicitly and backbone and side chain degrees of freedom are simultaneously optimized. The method recapitulates, often with very high accuracy, the structures of existing multidomain proteins.
authors.affiliation: Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Wollacott
authors.forename: Alexandre
authors.initials: A
authors.lastname: Zanghellini
authors.forename: Paul
authors.initials: P
authors.lastname: Murphy
authors.forename: David
authors.initials: D
authors.lastname: Baker
mesh_terms: D019295:Computational Biology / Q000379:methods*; D003198:Computer Simulation; D008958:Models, Molecular; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins