Colony spreading in Staphylococcus aureus.
Wild-type Staphylococcus aureus rapidly expands on the surface of soft agar plates. The rates of expansion and the shapes of the resultant giant colonies were distinct for different strains of laboratory stocks and clinical isolates. The colony spreading abilities did not correlate with the biofilm-forming abilities in these strains. Insertional disruption of the dltABCD operon, which functions at the step of D-alanine addition to teichoic acids, and of the tagO gene, which is responsible for the synthesis of wall teichoic acids, decreased the colony spreading ability. The results indicate that wall teichoic acids and D-alanylation of teichoic acids are required for colony spreading.
Kaito
Chikara
C
Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 3-1, 7-Chome, Hongo, Tokyo 113-0033, Japan.
Sekimizu
Kazuhisa
K
D000362:Agar; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D003470:Culture Media; D006801:Humans; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D009068:Movement; D009154:Mutation; D009876:Operon; D013211:Staphylococcus aureus / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism / Q000502:physiology*; D013682:Teichoic Acids / Q000378:metabolism*; D013869:Thiolester Hydrolases / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D002352:Carrier Proteins; D003470:Culture Media; C085651:D-alanyl carrier protein, bacteria; C118498:DltB protein, Staphylococcus; D026901:Membrane Transport Proteins; D013682:Teichoic Acids; D000362:Agar; C408973:DltD protein, bacteria; D013869:Thiolester Hydrolases