title: Ashwagandhanolide, a bioactive dimeric thiowithanolide isolated from the roots of Withania somnifera.
abstract: A new dimeric withanolide, ashwagandhanolide (1), was isolated from the roots of an Ayurvedic medicinal herb, Withania somnifera. A detailed spectroscopic evaluation revealed its identity as a dimer with an unusual thioether linkage. Compound 1 displayed growth inhibition against human gastric (AGS), breast (MCF-7), central nervous system (SF-268), colon (HCT-116), and lung (NCI H460) cancer cell lines, with IC50 values in the range 0.43-1.48 microg/mL. In addition, it inhibited lipid peroxidation and the activity of the enzyme cyclooxygenase-2 in vitro.
authors.affiliation: Laila Research Center, Unit I, Phase III, Jawahar Autonagar, Vijayawada 520 007, India. subbarajugottumukkala@hotmail.com
authors.forename: Gottumukkala V
authors.initials: GV
authors.lastname: Subbaraju
authors.forename: Mulabagal
authors.initials: M
authors.lastname: Vanisree
authors.forename: Chirravuri V
authors.initials: CV
authors.lastname: Rao
authors.forename: Chillara
authors.initials: C
authors.lastname: Sivaramakrishna
authors.forename: Pratha
authors.initials: P
authors.lastname: Sridhar
authors.forename: Bolleddula
authors.initials: B
authors.lastname: Jayaprakasam
authors.forename: Muraleedharan G
authors.initials: MG
authors.lastname: Nair
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D016861:Cyclooxygenase Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D004875:Ergosterol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D007194:India; D015227:Lipid Peroxidation / Q000187:drug effects; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D032341:Withania / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D016861:Cyclooxygenase Inhibitors; C516508:ashwagandhanolide; D004875:Ergosterol