SmaI cloning with regeneration of the SmaI site for sequential PCR product cloning.
Tachibana
Akira
A
Department of Bioengineering, Graduate School of Engineering, Osaka City University, Sugimoto 3-3-138, Sumiyoshi-ku, Osaka 558-8585, Japan. tatibana@bioa.eng.osaka-cu.ac.jp
Tanabe
Toshizumi
T
Yamauchi
Kiyoshi
K
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D015257:DNA-Cytosine Methylases / Q000235:genetics* / Q000378:metabolism; D016678:Genome; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods*; D011994:Recombinant Proteins / Q000032:analysis
D017931:DNA Primers; D011994:Recombinant Proteins; C079006:DNA modification methylase XcyI; D015257:DNA-Cytosine Methylases