title: "Cold-sensitive" mutants of the Lac repressor.
abstract: Thirteen of more than 4,000 single-amino-acid-replacement mutants of the Lac repressor, generated by suppression of amber nonsense mutants, were characterized as having a cold-sensitive phenotype. However, when expressed as missense mutations, none of the replacements cause cold sensitivity, implicating the suppression mechanism as being responsible for this phenotype.
authors.affiliation: Institut für Genetik der Universität zu Köln, Germany.
authors.forename: Andrew
authors.initials: A
authors.lastname: Barker
authors.forename: Stefan
authors.initials: S
authors.lastname: Oehler
authors.forename: Benno
authors.initials: B
authors.lastname: Müller-Hill
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000502:physiology; D018389:Codon, Nonsense; D003080:Cold Temperature; D056968:Lac Repressors; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D010641:Phenotype; D012097:Repressor Proteins / Q000737:chemistry* / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D018389:Codon, Nonsense; D056968:Lac Repressors; D012097:Repressor Proteins