Specific binding of D1 protein degradation products to the psbAI promoter in Synechococcus sp. strain PCC 7942.
The D1:1 protein and its potentially occurring degradation products were overexpressed in Escherichia coli. Protein-DNA interaction is shown for the promoter region of psbAI. The D1:1 degradation products may be involved in transcription regulation of psbAI by binding in the promoter region. Additionally, C-terminal fragments of the D1 protein bind to a sequence with similarity to isiB, a gene which encodes a flavodoxin-like protein.
Stelljes
Christian
C
Molekulare Pflanzenphysiologie, Universität Bremen, Leobener Str., 28359 Bremen, Germany.
Koenig
Friederike
F
D015153:Blotting, Western; D004247:DNA / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D045332:Photosystem II Protein Complex / Q000235:genetics* / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D046940:Synechococcus / Q000235:genetics*
D045332:Photosystem II Protein Complex; C422369:photosystem II, psbA subunit; D004247:DNA