Synaptic protein dynamics in hibernation.
Neurons in hibernating mammals exhibit a dramatic form of plasticity during torpor, with dendritic arbors retracting as body temperature cools and then regrowing rapidly as body temperature rises. In this study, we used immunohistochemical imaging and Western blotting of several presynaptic and postsynaptic proteins to determine the synaptic changes that accompany torpor and to investigate the mechanisms behind these changes. We show torpor-related alterations in synaptic protein localization that occur rapidly and uniformly across several brain regions in a temperature-dependent manner. Entry into torpor is associated with a 50-65% loss of synapses, as indicated by changes in the extent of colocalization of presynaptic and postsynaptic markers. We also show that the loss of synaptic protein clustering occurring during entry into torpor is not attributable to protein loss. These findings suggest that torpor-related changes in synapses stem from dissociation of proteins from the cytoskeletal active zone and postsynaptic density, creating a reservoir of proteins that can be quickly mobilized for rapid rebuilding of dendritic spines and synapses during the return to euthermia. A mechanism of neural plasticity based on protein dissociation rather than protein breakdown could explain the hibernator's capacity for large, rapid, and repeated microstructural changes, providing a fascinating contrast to neuropathologies that are dominated by protein breakdown and cell death.
von der Ohe
Christina G
CG
Department of Biological Sciences, Stanford University, Stanford, California 94305, USA. vonderohe@stanford.edu
Garner
Craig C
CC
Darian-Smith
Corinna
C
Heller
H Craig
HC
D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000648:ultrastructure; D006605:Hibernation / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009473:Neuronal Plasticity / Q000502:physiology*; D012589:Sciuridae; D013569:Synapses / Q000378:metabolism* / Q000648:ultrastructure; D014018:Tissue Distribution
D009419:Nerve Tissue Proteins