title: P2X7-induced apoptosis decreases by aging in mice myeloblasts.
abstract: In the current study, the ability of ATP to promote apoptosis in myeloblasts at different ages was investigated. We have observed that high concentration of extracellular ATP (>1mM), which activates P2X(7) receptor, produced cell shrinkage an increase in the number of events in the sub-G(0)/G(1) region of the cellular cycle and annexin-V/propidium iodide label, which characterizes the apoptotic cell death. In addition, BzATP produced apoptosis, but not ADP and UTP. Gr-1(+) cells express the P2X(7) receptor and oxidized ATP, a specific P2X(7) inhibitor, blocked the ATP-dependent apoptosis. ATP-dependent apoptosis is decreased by aging in myeloblasts of 12 and 22-month-old mice. Furthermore, P2X(7) expression decrease was observed in older mice, explaining apoptosis decrease. This decrease in apoptosis by aging may be related to some diseases in the myelocyte lineage.
authors.affiliation: Department of Biophysics, Federal University of São Paulo, R. Botucatu 862, Brazil. jean@biofis.epm.br <jean@biofis.epm.br>
authors.forename: Edgar Julian
authors.initials: EJ
authors.lastname: Paredes-Gamero
authors.forename: Juliana Luporini
authors.initials: JL
authors.lastname: Dreyfuss
authors.forename: Helena B
authors.initials: HB
authors.lastname: Nader
authors.forename: Maria Etsuko
authors.initials: ME
authors.lastname: Miyamoto Oshiro
authors.forename: Alice Teixeira
authors.initials: AT
authors.lastname: Ferreira
mesh_terms: D000244:Adenosine Diphosphate / Q000502:physiology; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology / Q000502:physiology; D000345:Affinity Labels / Q000494:pharmacology; D000375:Aging / Q000502:physiology*; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D042381:Granulocyte Precursor Cells / Q000502:physiology*; D006614:Hindlimb; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D058919:Purinergic P2 Receptor Antagonists; D018048:Receptors, Purinergic P2 / Q000502:physiology*; D058486:Receptors, Purinergic P2X7; D014544:Uridine Triphosphate / Q000502:physiology
chemical_list: D000345:Affinity Labels; C546469:P2rx7 protein, mouse; D058919:Purinergic P2 Receptor Antagonists; D018048:Receptors, Purinergic P2; D058486:Receptors, Purinergic P2X7; C017199:2',3'-dialdehyde ATP; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D014544:Uridine Triphosphate