[A novel microemulsion electrokinetic chromatography for measuring lipid-water partition coefficients of pharmaceuticals].
OBJECTIVE
To establish a novel microemulsion electrokinetic chromatography (MEEKC) method for measuring lipid-water partition coefficients ( logP(ow)) of pharmaceuticals without using microemulsion phase marker in order to avoid the error from tracing the migration time of microemulsion phase.


METHODS
The migration time of microemulsion phase (t(me)) was obtained by non-linearity fitting with logP(ow) values from literature and measured migration time (t(m)) of a series of organic compounds, a calibration curve for estimating logP(ow) of pharmaceuticals was thus obtained. In addition, the accuracy of the values measured by MEEKC was evaluated.


RESULTS
The logP(ow) values of 4 pharmaceuticals measured by MEEKC method presented in this paper were close to those determined by shake-flask method, and the average error between values from two methods was 0.15 logarithm units. Furthermore, according to the suggested theory, the measurement accuracy of logP(ow) is correlated with different t(m) in MEEKC.


CONCLUSIONS
The proposed method is simple, rapid, reproducible, and reliable with high measurement accuracy, which can be useful to estimate lipid-water partition coefficients of pharmaceuticals.
Jiang
Xue-Mei
XM
Institute of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, China.
Wei
Wei-Li
WL
Xia
Zhi-Ning
ZN
Chen
Zhi-Tao
ZT
D000082:Acetaminophen / Q000737:chemistry; D000212:Acyclovir / Q000737:chemistry; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D017292:Doxazosin / Q000737:chemistry; D004655:Emulsions; D008055:Lipids / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D015203:Reproducibility of Results; D014867:Water / Q000737:chemistry*
D004655:Emulsions; D008055:Lipids; D004364:Pharmaceutical Preparations; D014867:Water; D000082:Acetaminophen; D017292:Doxazosin; D000212:Acyclovir