Selective solid-phase extraction of tebuconazole in biological and environmental samples using molecularly imprinted polymers.
Molecularly imprinted polymers (MIPs) were prepared by precipitation polymerization using tebuconazole (TBZ) as a template. Frontal chromatography and selectivity experiments were used to determine the binding capabilities and binding specificities of different MIPs. The polymer that had the highest binding selectivity and capability was used as the solid-phase extraction (SPE) sorbent for the direct extraction of TBZ from different biological and environmental samples (cabbage, pannage, shrimp, orange juice and tap water). The extraction protocol was optimized and the optimum conditions were: conditioning with 5 mL methanol:acetic acid (9:1), 5 mL methanol and 5 mL water respectively, loading with 5 mL aqueous samples, washing with 1.2 mL acetonitrile (ACN):phosphate buffer (5:5, pH3), and eluting with 3 mL methanol. The MIPs were able to selectively recognize, effectively trap and preconcentrate TBZ over a concentration range of 0.5-15 micromol/L. The intraday and interday RSDs were less than 9.7% and 8.6%, respectively. The limit of quantification was 0.1 micromol/L. Under optimum conditions, the MISPE recoveries of spiked cabbage, pannage, shrimp, orange juice and tap water were 62.3%, 75.8%, 71.6%, 89% and 93.9%, respectively. MISPE gave better HPLC separation efficiencies and higher recoveries than C18 SPE and strong cation exchange (SCX) SPE.
Hu
Mai-ling
ML
Key Laboratory of Environment & Health of Ministry of Education, Institute of Environmental Medicine, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, People's Republic of China.
Jiang
Ming
M
Wang
Peng
P
Mei
Su-rong
SR
Lin
Yan-fei
YF
Hu
Xiao-zhong
XZ
Shi
Yun
Y
Lu
Bin
B
Dai
Kang
K
D019342:Acetic Acid / Q000737:chemistry; D000097:Acetonitriles / Q000737:chemistry; D002021:Buffers; D002411:Cation Exchange Resins; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005504:Food Analysis / Q000379:methods*; D000432:Methanol / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*; D013997:Time Factors; D014230:Triazoles / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis*
D000097:Acetonitriles; D002021:Buffers; D002411:Cation Exchange Resins; D011108:Polymers; D014230:Triazoles; D014874:Water Pollutants, Chemical; C087114:tebuconazole; D019342:Acetic Acid; D000432:Methanol; C032159:acetonitrile