Are free radical reactions increased in the diabetic eye?
Reactive oxygen species (ROS) are thought to play a significant role in the development of diabetic retinopathy; however, no direct evidence supports ROS generation in vivo. This study used in vivo electron spin resonance (ESR) spectroscopy with a surface resonator to detect local free radical reactions. The ESR signal decay of carbamoyl-PROXYL was enhanced in the eyes of streptozotocin (STZ)-induced diabetic mice. This enhanced signal decay was suppressed by the administration of SOD or the pretreatment with aminoguanidine. We demonstrate, for the first time, specific free radical reactions in the eyes of mice with STZ-induced diabetes.
Yamato
Mayumi
M
Department REDOX Medicinal Science, Faculty of Pharmaceutical Sciences, Graduate School of Medical Science, Kyushu University, Fukuoka, Japan.
Matsumoto
Shingo
S
Ura
Katsuaki
K
Yamada
Ken-Ichi
K
Naganuma
Tatsuya
T
Inoguchi
Toyoshi
T
Watanabe
Toshiaki
T
Utsumi
Hideo
H
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D003930:Diabetic Retinopathy / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D005123:Eye / Q000378:metabolism*; D005609:Free Radicals / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D051379:Mice; D018384:Oxidative Stress; D013113:Spin Labels; D013311:Streptozocin
D005609:Free Radicals; D013113:Spin Labels; D013311:Streptozocin; D005947:Glucose