RNA interference in mice.
Silencing of gene expression by RNA interference (RNAi) has become a powerful tool for functional genomics in mammalian cells. Furthermore, RNAi holds promise as a simple, fast and cost-effective approach to studying mammalian gene function in vivo and as a novel therapeutic approach. This review provides an overview of the progress of RNAi in vivo, with emphasis on systemic/local siRNA delivery, viral shRNA vectors, shRNA vector transgenic mice and conditional systems to control shRNA vectors. Taken together, the data from 80 in vivo studies show that RNAi is a useful tool that offers new opportunities for functional genomics in mice.
Kühn
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Institute for Developmental Genetics, GSF, National Research Center for Environment and Health, Ingolstädter Landstr. 1, 85764 Neuherberg, Germany. ralf.kuehn@gsf.de
Streif
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S
Wurst
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D000818:Animals; D005822:Genetic Vectors; D051379:Mice; D008822:Mice, Transgenic; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism
D034741:RNA, Small Interfering; D012367:RNA, Viral