Expression, purification and preliminary crystallographic studies on the catalytic region of the nonreceptor tyrosine kinase Fes.
The proto-oncogene tyrosine protein kinase c-fps/fes encodes a structurally unique protein (Fes) of the nonreceptor protein-tyrosine kinase (PTK) family. Its expression has been demonstrated in myeloid haematopoietic cells, vascular endothelial cells and in neurons. In human-derived and murine-derived cell lines, the activated form of this kinase can induce cellular transformation; moreover, it has been shown that Fes is involved in the regulation of cell-cell and cell-matrix interactions mediated by adherens junctions and focal adhesions. The N-terminus of Fes contains the FCH (Fps/Fes/Fer/CIP4 homology) domain, which is unique to the Fes/Fer kinase family. It is followed by three coiled-coil domains and an SH2 (Src-homology 2) domain. The catalytic region (Fes-CR) is located at the C-terminus of the protein. The successful expression, purification and crystallization of the catalytic part of Fes (Fes-CR) are described.
Gnemmi
Ilaria
I
DiSCAFF&DFB Center, Università del Piemonte Orientale A. Avogadro, Via Giovanni Bovio 6, 28100, Novara, Italy.
Scotti
Claudia
C
Cappelletti
Donata
D
Canonico
Pier Luigi
PL
Condorelli
Fabrizio
F
Rosano
Camillo
C
D000595:Amino Acid Sequence; D000818:Animals; D020134:Catalytic Domain / Q000502:physiology*; D018360:Crystallography, X-Ray / Q000379:methods*; D029968:Escherichia coli Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D000090063:Proto-Oncogene Mas; D051578:Proto-Oncogene Proteins c-fes / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification
D029968:Escherichia coli Proteins; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D051578:Proto-Oncogene Proteins c-fes