title: Effects of altered gravity on the actin and microtubule cytoskeleton of human SH-SY5Y neuroblastoma cells.
abstract: Human SH-SY5Y neuroblastoma cells were used to study the effects of altered gravity on the actin and microtubule cytoskeleton dynamics. A cholinergic stimulation of the cells during a 6 min period of changing gravity (3 parabolas) resulted in an enhanced actin-driven protrusion of evoked lamellipodia. Likewise, the spontaneous protrusive activity of nonactivated cells was promoted during exposure to changing gravity (6 up to 31 parabolas). Ground-based experiments revealed a similar enhancement of the spontaneous and evoked lamellar protrusive activity when the cells were kept at 2 g hypergravity for at least 6 min. This gravity response was independent of the direction of the acceleration vector in respect to the cells. Exposure of the cells to "simulated weightlessness" (clinorotation) had no obvious influence on this type of lamellar actin cytoskeleton dynamics. A 20 min exposure of the cells to simulated weightlessness or to changing gravity (6 to 31 parabolas) - but not to 2 g (hypergravity, centrifugation) - resulted in an altered arrangement of microtubules indicated by bending, turning, and loop formation. A similar altered arrangement was shown by microtubules which had polymerized into lamellipodia after release from a taxol block at simulated weightlessness (clinorotation) or during changing gravity (5 parabolas). Our data suggest that in human SH-SY5Y neuroblastoma cells, microgravity affects the dynamics and spatial arrangement of microtubules but has no influence on the Rac-controlled lamellar actin cytoskeleton dynamics and cell spreading. The latter, however, seems to be promoted at hypergravity.
authors.affiliation: Cell and Developmental Neurobiology, Institute of Zoology, University of Hohenheim, Stuttgart, Federal Republic of Germany. roesnerh@uni-hohenheim.de
authors.forename: H
authors.initials: H
authors.lastname: Rösner
authors.forename: T
authors.initials: T
authors.lastname: Wassermann
authors.forename: W
authors.initials: W
authors.lastname: Möller
authors.forename: W
authors.initials: W
authors.lastname: Hanke
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D008841:Actin Cytoskeleton / Q000187:drug effects / Q000378:metabolism*; D045744:Cell Line, Tumor; D002498:Centrifugation; D018679:Cholinergic Agonists / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism*; D018466:Gravity Sensing; D018470:Gravity, Altered; D006801:Humans; D018471:Hypergravity; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism*; D009447:Neuroblastoma; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D011554:Pseudopodia / Q000378:metabolism; D012399:Rotation; D013026:Space Flight; D013997:Time Factors; D014893:Weightlessness; D018474:Weightlessness Simulation
chemical_list: D018679:Cholinergic Agonists; D000109:Acetylcholine