title: Parallel manipulation of bifunctional DNA molecules on structured surfaces using kinesin-driven microtubules.
abstract: We have developed a technique to manipulate bifunctional DNA molecules: One end is thiolated to bind to a patterned gold surface and the other end is biotinylated to bind to a microtubule gliding over a kinesin-coated surface. We found that DNA molecules can be stretched and overstretched between the gold pads and the motile microtubules, and that they can form dynamic networks. This serves as a proof-of-principle that biological machineries can be used in vitro to accomplish the parallel formation of structured DNA templates that will have applications in biophysics and nanoelectronics.
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.
authors.forename: Cerasela Zoica
authors.initials: CZ
authors.lastname: Dinu
authors.forename: Jörg
authors.initials: J
authors.lastname: Opitz
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Pompe
authors.forename: Jonathon
authors.initials: J
authors.lastname: Howard
authors.forename: Michael
authors.initials: M
authors.lastname: Mertig
authors.forename: Stefan
authors.initials: S
authors.lastname: Diez
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics; D004279:DNA, Viral / Q000378:metabolism*; D006046:Gold; D016547:Kinesins / Q000502:physiology*; D008870:Microtubules / Q000378:metabolism; D036103:Nanotechnology
chemical_list: D004279:DNA, Viral; D006046:Gold; D016547:Kinesins