Establishment and evaluation of 2 monoclonal antibodies against oxidized apolipoprotein A-I (apoA-I) and its application to determine blood oxidized apoA-I levels.
BACKGROUND
Apolipoprotein A-I (apoA-I) is the major lipoprotein component of high-density lipoprotein(HDL), and plays an important role in reverse cholesterol transport. Its function is known to be influenced by oxidation.


METHODS
Using H2O2-or chloramine T-oxidized apoA-I as antigen, we prepared 2 kinds of monoclonal antibodies, and established an ELISA system for the measurement of oxidized apoA-I.


RESULTS
The 2 monoclonal antibodies obtained, 7D3 and 98A7, exhibited different reactivity characteristics. The serum level of oxidized apoA-I was higher in patients with either inflammatory disease or diabetes than in healthy individuals, and suggested a diversity of oxidized apoA-I.


CONCLUSIONS
The 2 monoclonal antibodies are useful for the determination of oxidized apoA-I and study of diverse oxidized HDLs.
Ueda
Masashi
M
Ikagaku Co. Ltd., Furukawacho, 328 Hazukashi Fushimi-ku, Kyoto 612-8486, Japan. ueda@ikagaku.co.jp
Hayase
Yasuyuki
Y
Mashiba
Shinichi
S
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal / Q000276:immunology*; D016632:Apolipoprotein A-I / Q000097:blood* / Q000302:isolation & purification / Q000378:metabolism*; D002700:Chloramines / Q000737:chemistry; D002846:Chromatography, Affinity; D003920:Diabetes Mellitus / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood; D008075:Lipoproteins, HDL / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D014105:Tosyl Compounds / Q000737:chemistry
D000911:Antibodies, Monoclonal; D016632:Apolipoprotein A-I; D002700:Chloramines; D008075:Lipoproteins, HDL; D014105:Tosyl Compounds; C016300:chloramine-T