Molecular authentication of Radix Puerariae Lobatae and Radix Puerariae Thomsonii by ITS and 5S rRNA spacer sequencing.
In the present study, we examined nuclear DNA sequences in an attempt to reveal the relationships between Pueraria lobata (Willd). Ohwi, P. thomsonii Benth., and P. montana (Lour.) Merr. We found that internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA are highly divergent in P. lobata and P. thomsonii, and four types of ITS with different length are found in the two species. On the other hand, DNA sequences of 5S rRNA gene spacer are highly conserved across multiple copies in P. lobata and P. thomsonii, they could be used to identify P. lobata, P. thomsonii, and P. montana of this complex, and may serve as a useful tool in medical authentication of Radix Puerariae Lobatae and Radix Puerariae Thomsonii.
Sun
Ye
Y
Department of Biochemistry and Institute of Chinese Medicine, The Chinese University of Hong Kong, NT.
Shaw
Pang-Chui
PC
Fung
Kwok-Pui
KP
D018744:DNA, Plant / Q000032:analysis / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000032:analysis / Q000235:genetics*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000592:standards; D019143:Evolution, Molecular; D018517:Plant Roots; D029908:Pueraria / Q000145:classification / Q000235:genetics*; D011786:Quality Control; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D017422:Sequence Analysis, DNA
D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer; D004365:Drugs, Chinese Herbal; D012341:RNA, Ribosomal, 5S; C442842:plant extract, Puerariae Radix