title: Automated Tecan programming for bioanalytical sample preparation with EZTecan.
abstract: This paper reports the first successful automated Tecan (Tecan US, Research Triangle Park, NC) programming for bioanalytical sample analysis with an in-house developed software, called EZTecan. Based on the sample run list in the Watson Laboratory Information Management System (InnaPhase Corp., Philadelphia, PA), EZTecan can generate a Tecan program for bioanalytical sample preparation, including sample transfer, sample dilution, extraction, and reconstitution. Many built-in functions for EZTecan ensure the flexibility, quality, and efficiency of the liquid handling. It takes about 90 min to prepare two 96-well plates from sample transfer to reconstitution. Analytical results shown good inter- and intra-assay accuracy and precision, which meet the +/-15% Good Laboratory Performance acceptance criteria. With EZTecan, bioanalysts can prepare samples without writing a single line of Tecan program.
authors.affiliation: Pharmaceutical Research Institute, Bristol Myers Squibb Company, Princeton, NJ 08543, USA. huidong.gu@bms.com
authors.forename: Huidong
authors.initials: H
authors.lastname: Gu
authors.forename: Steve
authors.initials: S
authors.lastname: Unger
authors.forename: Yuzhong
authors.initials: Y
authors.lastname: Deng
mesh_terms: D001331:Automation; D001681:Biological Assay / Q000295:instrumentation*; D019451:Information Management; D012984:Software