title: Are free radical reactions increased in the diabetic eye?
abstract: Reactive oxygen species (ROS) are thought to play a significant role in the development of diabetic retinopathy; however, no direct evidence supports ROS generation in vivo. This study used in vivo electron spin resonance (ESR) spectroscopy with a surface resonator to detect local free radical reactions. The ESR signal decay of carbamoyl-PROXYL was enhanced in the eyes of streptozotocin (STZ)-induced diabetic mice. This enhanced signal decay was suppressed by the administration of SOD or the pretreatment with aminoguanidine. We demonstrate, for the first time, specific free radical reactions in the eyes of mice with STZ-induced diabetes.
authors.affiliation: Department REDOX Medicinal Science, Faculty of Pharmaceutical Sciences, Graduate School of Medical Science, Kyushu University, Fukuoka, Japan.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Yamato
authors.forename: Shingo
authors.initials: S
authors.lastname: Matsumoto
authors.forename: Katsuaki
authors.initials: K
authors.lastname: Ura
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Yamada
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Naganuma
authors.forename: Toyoshi
authors.initials: T
authors.lastname: Inoguchi
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Watanabe
authors.forename: Hideo
authors.initials: H
authors.lastname: Utsumi
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D003930:Diabetic Retinopathy / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D005123:Eye / Q000378:metabolism*; D005609:Free Radicals / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D051379:Mice; D018384:Oxidative Stress; D013113:Spin Labels; D013311:Streptozocin
chemical_list: D005609:Free Radicals; D013113:Spin Labels; D013311:Streptozocin; D005947:Glucose