title: Prevention of diabetic microangiopathy by prophylactic transplant of mobilized peripheral blood mononuclear cells.
abstract: OBJECTIVE
  To investigate whether the prophylactic local delivery of mobilized peripheral blood mononuclear cells (M-PBMNC) could prevent peripheral microangiopathy in diabetic nude mice.
  
  
  METHODS
  Diabetic nude mice were induced with intraperitoneal injections of streptozotocin. With the time course of diabetes, we detected the capillary and arteriole density of mice adductor muscles by immunohistopathy. In situ apoptosis was detected by using TdT-mediated dUTP nick end labeling (TUNEL) methods. M-PBMNC were labeled and locally delivered to the adductor muscles. Mononuclear cells were also isolated and cultured in vitro for the detection and counting of endothelial progenitor cells(EPC).
  
  
  RESULTS
  Rarefication of capillaries and arterioles, enhanced apoptosis in adductor muscles, and reduced circulating EPC in diabetic nude mice. Prophylactic local delivery of M-PBMNC halted the progression of microvascular rarefaction in hind-limb skeletal muscles by inhibiting apoptosis. We detected the survival, migration and incorporation of transplanted M-PBMNC into the murine vasculature in vivo. In addition, more EPC were available from M-PBMNC than non-mobilized cells.
  
  
  CONCLUSIONS
  These results suggested that the prophylactic local delivery of M-PBMNC may represent a novel approach for the treatment of microvascular complications in diabetics.
authors.affiliation: State Key Laboratory of Experimental Hematology, National Research Center for Stem Cell Engineering and Technology, Institute of Hematology, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Xiao-Cang
authors.initials: XC
authors.lastname: Cao
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Fang
authors.forename: Cui-Lin
authors.initials: CL
authors.lastname: Zheng
authors.forename: Zhi-Bo
authors.initials: ZB
authors.lastname: Han
authors.forename: He
authors.initials: H
authors.lastname: Ren
authors.forename: Man-Chiu
authors.initials: MC
authors.lastname: Poon
authors.forename: Zhong-Chao
authors.initials: ZC
authors.lastname: Han
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001160:Arterioles / Q000187:drug effects / Q000473:pathology; D002196:Capillaries / Q000187:drug effects / Q000473:pathology; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D003925:Diabetic Angiopathies / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D042783:Endothelial Cells / Q000473:pathology; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D019650:Hematopoietic Stem Cell Mobilization; D006412:Hematopoietic Stem Cells / Q000473:pathology; D006614:Hindlimb / Q000098:blood supply; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000637:transplantation*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009389:Neovascularization, Pathologic / Q000517:prevention & control
chemical_list: D016179:Granulocyte Colony-Stimulating Factor