Simultaneous measurement of intracellular triphosphate metabolites of zidovudine, lamivudine and abacavir (carbovir) in human peripheral blood mononuclear cells by combined anion exchange solid phase extraction and LC-MS/MS.
All nucleoside reverse transcriptase inhibitors (NRTI) must first be metabolized to their triphosphate forms in order to be active against HIV. Zidovudine (ZDV), abacavir (ABC) and lamivudine (3TC) have proven to be an efficacious combination. In order simultaneously to measure intracellular levels of the triphosphates (-TP) of ZDV, ABC (carbovir, CBV) and 3TC, either together or individually, we have developed a cartridge-LC-MS/MS method. The quantitation range was 2.5-250 pg/microl for 3TC-TP, 0.1-10.0 pg/microl for ZDV-TP and 0.05-5.00 pg/microl for CBV-TP. This corresponds to 0.1-11.0 pmol 3TC-TP per million cells, 4-375 fmol ZDV-TP per million cells and 2-200 fmol CBV-TP per million cells, extracted from 10 million cells. Patient samples demonstrated measured levels in the middle regions of our standard curves both at pre-dose and 4h post-dose times.
Robbins
Brian L
BL
Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, TN, USA. brian.robbins@stjude.org
Poston
Philip A
PA
Neal
Erin F
EF
Slaughter
Clive
C
Rodman
John H
JH
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015224:Dideoxynucleosides / Q000097:blood*; D015658:HIV Infections / Q000097:blood; D006801:Humans; D019259:Lamivudine / Q000097:blood*; D010710:Phosphates / Q000097:blood*; D012015:Reference Standards; D018894:Reverse Transcriptase Inhibitors / Q000097:blood*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D015215:Zidovudine / Q000097:blood*; D000099227:Abacavir
D015224:Dideoxynucleosides; D019259:Lamivudine; D010710:Phosphates; D018894:Reverse Transcriptase Inhibitors; D015215:Zidovudine; D000099227:Abacavir