Yeast two-hybrid approaches to dissecting the plant defense response.
We describe a reliable GAL4-based yeast two-hybrid system for identifying and isolating clones encoding proteins interacting with a protein of interest. This two-hybrid system gives extremely low background and few false-positive clones, making it ideal for library screening purposes. We have successfully used it not only to isolate Arabidopsis NPR1-interactors from rice but also to pull out the rice NPR1 ortholog using one of the interactors as bait.
Chern
Mawsheng
M
Department of Plant Pathology, University of California, Davis, USA.
Richter
Todd
T
Ronald
Pamela C
PC
D017360:Arabidopsis / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D018744:DNA, Plant / Q000302:isolation & purification; D015723:Gene Library; D007113:Immunity, Innate / Q000502:physiology*; D012275:Oryza / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D010935:Plant Diseases; D010957:Plasmids / Q000302:isolation & purification; D014170:Transformation, Genetic; D020798:Two-Hybrid System Techniques; D015003:Yeasts / Q000166:cytology; D001616:beta-Galactosidase / Q000378:metabolism
D018744:DNA, Plant; D001616:beta-Galactosidase