title: Nanostructured bio-functional polymer brushes.
abstract: Structured poly(glycidyl methracrylate) (poly-GMA) brushes have been grafted onto flexible fluoro-polymer films using a radiation grafting process. The reactive epoxide of poly-GMA provides the basis for a versatile biofunctionalization of the grafted brushes. Structure definition by extreme ultraviolet (EUV) exposure allowed nanometer-scale resolution of periodic patterns. By variation of the exposure dose the height of the grafted structures can be adapted in a wide range. Derivatization of the grafted brushes included reaction with various amines with different side chains, hydrolysis of the epoxide to diols to increase protein resistance and introduction of ionic groups to yield poly-electrolytes. As an example for biofunctionalization, biotin was linked to the grafted brush and biofunctionality was demonstrated in a competitive biotin-streptavidin assay. In this article we also present a brief review of other approaches to obtain structured biofunctional polymer brushes.
authors.affiliation: Laboratory for Micro- and Nanotechnology, Paul Scherrer Institut, 5232 Villigen PSI, Switzerland. celestino.padeste@psi.ch
authors.forename: Celestino
authors.initials: C
authors.lastname: Padeste
authors.forename: Patrick
authors.initials: P
authors.lastname: Farquet
authors.forename: Christian
authors.initials: C
authors.lastname: Potzner
authors.forename: Harun H
authors.initials: HH
authors.lastname: Solak
mesh_terms: D005466:Fluorocarbons; D018625:Microscopy, Atomic Force; D049329:Nanostructures; D036103:Nanotechnology / Q000295:instrumentation* / Q000639:trends; D011109:Polymethacrylic Acids / Q000737:chemistry*; D011138:Polytetrafluoroethylene / Q000031:analogs & derivatives
chemical_list: D005466:Fluorocarbons; D011109:Polymethacrylic Acids; C040378:poly(ethylene-tetrafluoroethylene); D011138:Polytetrafluoroethylene; C042535:polyglycidyl methacrylate