Full-length sequencing and genomic characterization of Bagaza, Kedougou, and Zika viruses.
Many members of the genus Flavivirus are the agents of important diseases of humans, livestock, and wildlife. Currently, no complete genome sequence is available for the three African viruses, Bagaza, Zika, and Kedougou viruses, each representing a distinct virus subgroup according to the latest virus classification. In this study, we obtained a complete genome sequence of each of those three viruses and characterized the open reading frames (ORFs) with respect to gene sizes, cleavage sites, potential glycosylation sites, distribution of cysteine residues, and unique motifs. The sequences of the three viruses were then scanned across the entire length of the ORF against available sequences of other African flaviviruses and selected reference viruses for genetic relatedness. The data collectively indicated that Kedougou virus was close to dengue viruses but nonetheless distinct, while Bagaza virus shared genetic relatedness with West Nile virus in several genomic regions. In the non-coding regions, it was found that a particular organizational pattern of conserved sequences in the 3' terminal region generally correlated with the current virus grouping.
Kuno
G
G
Arbovirus Diseases Branch, Division of Vector-Borne Infectious Diseases, National Center for Zoonotic, Vector-Borne, and Enteric Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80522-2087, USA. gok1@cdc.gov
Chang
G-J J
GJ
D020413:3' Untranslated Regions / Q000235:genetics; D001483:Base Sequence; D017124:Conserved Sequence; D018076:DNA, Complementary; D003716:Dengue Virus / Q000235:genetics; D018067:Flaviviridae / Q000145:classification / Q000235:genetics*; D016679:Genome, Viral; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014764:Viral Proteins / Q000235:genetics*; D014902:West Nile virus / Q000235:genetics; D000071244:Zika Virus / Q000145:classification / Q000235:genetics*
D020413:3' Untranslated Regions; D018076:DNA, Complementary; D012367:RNA, Viral; D014764:Viral Proteins