title: Platelet adhesion to human umbilical vein endothelial cells cultured on anionic hydrogel scaffolds.
abstract: In this work we describe experiments designed to understand the human platelet adhesion to human umbilical vein endothelial cells (HUVECs) cultured on various kinds of chemically cross-linked anionic hydrogels, which were synthesized by radical polymerization. HUVECs could proliferate to sub-confluent or confluent on poly(acrylic acid) (PAA), poly(2-acrylamido-2-methyl-propane sulfonic acid sodium salt) (PNaAMPS), and poly(sodium p-styrene sulfonate) (PNaSS) gels. The proliferation behavior was not sensitive to the cross-linker concentration of the gels. However, the platelet adhesion on the HUVECs cultured on these gels showed different behavior, as revealed by human platelet adhesion test in static conditions. Only a few platelets adhered on the HUVEC sheets cultured on PNaAMPS gels with 4 and 10mol% cross-linker concentrations, and completely no platelet adhered on the HUVEC sheets cultured on PNaSS gels with 4 and 10mol% cross-linker concentrations. On the other hand, a large number of platelets adhered on the HUVECs cultured on PAA gels with 1, 2mol% cross-linker concentrations and PNaAMPS gel with 2mol% cross-linker concentration. Furthermore, the study showed that promote of the glycocalyx of HUVECs with transforming growth factor-beta(1) (TGF-beta(1)) decreased platelet adhesion, and degrade the glycocalyx with heparinase I increased platelet adhesion. The results suggested that the glycocalyx of cultured HUVECs modulates platelet compatibility, and the amount of glycocalyx secreted by HUVECs dependents on the chemical structure and cross-linker concentration of gel scaffolds. This result should be applied to make the hybrid artificial blood vessel composes of gels and endothelial cells with high platelet compatibility.
authors.affiliation: Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan.
authors.forename: Yong Mei
authors.initials: YM
authors.lastname: Chen
authors.forename: Masaru
authors.initials: M
authors.lastname: Tanaka
authors.forename: Jian Ping
authors.initials: JP
authors.lastname: Gong
authors.forename: Kazunori
authors.initials: K
authors.lastname: Yasuda
authors.forename: Sadaaki
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Masatsugu
authors.initials: M
authors.lastname: Shimomura
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Osada
mesh_terms: D000838:Anions; D001672:Biocompatible Materials / Q000737:chemistry*; D001792:Blood Platelets / Q000166:cytology* / Q000502:physiology*; D002450:Cell Communication / Q000502:physiology; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology* / Q000502:physiology*; D019276:Glycocalyx / Q000378:metabolism; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D008422:Materials Testing; D010973:Platelet Adhesiveness / Q000502:physiology*; D014471:Umbilical Veins / Q000166:cytology / Q000502:physiology
chemical_list: D000838:Anions; D001672:Biocompatible Materials; D020100:Hydrogels