title: Pretreatment of polyamide monofilament with hydrochloric acid improves sensitivity of three-dimensional microarray, Bio-Strand.
abstract: A single-nucleotide-polymorphism-typing method using a novel three-dimensional DNA microarray, Bio-Strand, is promising because it is rapid, inexpensive and easily automated. It has been developed with the intent to overcome the drawbacks of conventional DNA microarrays, which use flat surfaces and impermeable materials such as glass slides; Bio-Strand as a novel DNA microarray, with its permeability, has a significantly improved stability compared with conventional DNA microarrays that use impermeable materials. In this study, we have developed a simple method of pretreating a polyamide monofilament to increase its surface area and to make it permeable, which makes Bio-Strand more sensitive and stable, allowing it to be adapted for clinical diagnostic applications. The fluorescence signal obtained with a nylon 6 monofilament pretreated under optimal conditions (hydrolysis by 5 M HCl/ethanol followed by washing with 50% ethanol and 100% ethanol) was significantly stronger than that obtained with an untreated monofilament.
authors.affiliation: Precision System Science Co., Ltd., 88 Kamihongo, Matsudo-shi, Chiba 271-0064, Japan. tojo@pss.co.jp
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Tojo
authors.forename: Reishi
authors.initials: R
authors.lastname: Syou
authors.forename: Mamiko
authors.initials: M
authors.lastname: Yoshida
authors.forename: Junko
authors.initials: J
authors.lastname: Momose
authors.forename: Harumi
authors.initials: H
authors.lastname: Ginya
authors.forename: Masaaki
authors.initials: M
authors.lastname: Takahashi
authors.forename: Hideji
authors.initials: H
authors.lastname: Tajima
authors.forename: Masafumi
authors.initials: M
authors.lastname: Yohda
mesh_terms: D006851:Hydrochloric Acid / Q000494:pharmacology*; D009757:Nylons / Q000494:pharmacology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D009757:Nylons; D006851:Hydrochloric Acid