title: Rapid species determination of Nocardia keratitis using pyrosequencing technology.
abstract: OBJECTIVE
  To describe a new technique, pyrosequencing, which allows for the rapid identification of Mycobacterium and Nocardia species.
  
  
  METHODS
  Interventional case report.
  
  
  METHODS
  The medical records of a patient presenting with an infectious keratitis were reviewed.
  
  
  RESULTS
  A case of Nocardia abscessus/arthrititis/asiatica keratitis was diagnosed in a young individual with the aid of pyrosequencing technology. Based on presumed antibiotic sensitivities, therapy with topical trimethoprim-sulfamethoxazole eyedrops was initiated, and the infection was cleared rapidly with minimal residual scarring.
  
  
  CONCLUSIONS
  Pyrosequencing may be a useful tool in aiding the rapid diagnosis and treatment of ocular infections caused by slow-growing pathogens.
authors.affiliation: Cole Eye Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA.
authors.forename: Anat
authors.initials: A
authors.lastname: Galor
authors.forename: Gerri S
authors.initials: GS
authors.lastname: Hall
authors.forename: Gary W
authors.initials: GW
authors.lastname: Procop
authors.forename: Marion
authors.initials: M
authors.lastname: Tuohy
authors.forename: Michael E
authors.initials: ME
authors.lastname: Millstein
authors.forename: Bennie H
authors.initials: BH
authors.lastname: Jeng
mesh_terms: D000328:Adult; D000890:Anti-Infective Agents / Q000627:therapeutic use; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis*; D015818:Eye Infections, Bacterial / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*; D006801:Humans; D007634:Keratitis / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*; D008297:Male; D008969:Molecular Sequence Data; D009615:Nocardia / Q000235:genetics* / Q000302:isolation & purification; D009617:Nocardia Infections / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*; D009883:Ophthalmic Solutions / Q000627:therapeutic use; D017422:Sequence Analysis, DNA / Q000379:methods*; D015662:Trimethoprim, Sulfamethoxazole Drug Combination / Q000627:therapeutic use
chemical_list: D000890:Anti-Infective Agents; D004269:DNA, Bacterial; D009883:Ophthalmic Solutions; D015662:Trimethoprim, Sulfamethoxazole Drug Combination