Comparison of structure, strength and cytocompatibility of a fibrin matrix supplemented either with tranexamic acid or aprotinin.
Fibrin sealants are used as hemostats, sealants, tissue adhesives, and as matrix for substances/cells in a number of surgical and tissue engineering procedures. Main characteristics of fibrin are high tensile strength, adhesive strength, biocompatibility, and resorption. A major adverse event would be premature fibrin lysis and recurrent bleeding. This must be prevented by fibrinolysis inhibitors. The most common fibrinolysis inhibitors used are aprotinin and tranexamic acid (t-AMCA). Comparison of commercially available fibrin sealants utilizing aprotinin or t-AMCA revealed a lower sealing efficacy in an in vivo lung resection model for a t-AMCA containing product. Therefore, we compared the influence of t-AMCA and aprotinin on structure, mechanical properties, and cytocompatibility of a fibrin matrix. In our experiments, we found that substitution of aprotinin with t-AMCA reduced the tensile strength and formation of fibrin fibers and affected viability of a fibroblast cell-line. In conclusion, t-AMCA negatively affects physical and biological properties of fibrin relevant for clinical application as well as tissue regeneration.
Furst
Walter
W
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, The Research Center for Traumatology of the AUVA, Donaueschingenstrasse 13, A-1200 Vienna, Austria. walter.fuerst@lbitrauma.org
Banerjee
Asmita
A
Redl
Heinz
H
D000818:Animals; D000933:Antifibrinolytic Agents / Q000494:pharmacology* / Q000633:toxicity; D007611:Aprotinin / Q000494:pharmacology* / Q000633:toxicity; D005337:Fibrin / Q000187:drug effects* / Q000276:immunology / Q000648:ultrastructure; D015718:Fibrin Tissue Adhesive / Q000276:immunology / Q000494:pharmacology; D055370:Lung Injury; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D011817:Rabbits; D013718:Tensile Strength; D014148:Tranexamic Acid / Q000494:pharmacology* / Q000633:toxicity
D000933:Antifibrinolytic Agents; D015718:Fibrin Tissue Adhesive; D014148:Tranexamic Acid; D005337:Fibrin; D007611:Aprotinin