title: [Experimental study on the effects of jujingwan on oligospermia].
abstract: OBJECTIVE
  To investigate the effects of Jujingwan on the spermatozoal ultrastructure and apoptosis of germ cells in oligospermia patients.
  
  
  METHODS
  We treated 50 oligospermia patients with Jujingwan and observed the spermatozoal ultrastructure, the apoptosis of germ cells and the changes in the DNA ploidy proportion of spermatogenic cells by electron microscopy and FCM before the treatment and 3, 6, 9 and 12 months after it.
  
  
  RESULTS
  Jujingwan increased sperm acrosome base density 6 months after the treatment and remarkably improved the integrity of acrosome membrane 12 months after it, with no obvious pathological changes in the nuclei and tails. Three months after the treatment, cell debris and apoptotic cells decreased significantly as compared with pre-treatment (P < 0. 05) , and very significantly 12 months after the treatment (P <0. 01). The proportion of haploid spermatozoa increased very significantly (P <0.01) , and the lost primary spermatocytes decreased significantly (P <0. 05) compared with pre-treatment.
  
  
  CONCLUSIONS
  Jujingwan can increase the density of sperm acrosome base and improve the pathological changes of acrosome membrane in oligospermia patients; it can improve the activity of acrosome enzyme and the integrity of acrosome membrane, decrease the apoptosis rate of germ cells and sperm and increase the percentage of haploid spermatozoa; it can also reduce the percentage of apoptotic bodies and diploid sperm cells. It is indicated that Jujingwan can inhibit the apoptosis of germ cells and sperm and improve spermatogenesis in oligospermia patients.
authors.affiliation: Department of Andrology, Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing , Jiangsu 210029, China. swkr@sohu.com
authors.forename: Ying
authors.initials: Y
authors.lastname: He
authors.forename: Yan
authors.initials: Y
authors.lastname: Cao
authors.forename: Yu-jian
authors.initials: YJ
authors.lastname: Xu
authors.forename: Jian
authors.initials: J
authors.lastname: Huang
authors.forename: Zhi-xing
authors.initials: ZX
authors.lastname: Sun
authors.forename: Zuo-min
authors.initials: ZM
authors.lastname: Zhou
authors.forename: Jia-hao
authors.initials: JH
authors.lastname: Sha
authors.forename: Cheng-yong
authors.initials: CY
authors.lastname: Liu
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Chi
authors.forename: Xiao-yu
authors.initials: XY
authors.lastname: Yang
mesh_terms: D000177:Acrosome / Q000473:pathology; D000328:Adult; D017209:Apoptosis; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D006801:Humans; D007248:Infertility, Male / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D009845:Oligospermia / Q000188:drug therapy* / Q000473:pathology; D008517:Phytotherapy; D013076:Sperm Count; D013090:Spermatocytes / Q000166:cytology; D013094:Spermatozoa / Q000648:ultrastructure
chemical_list: D004365:Drugs, Chinese Herbal