title: RBMX is a novel hepatic transcriptional regulator of SREBP-1c gene response to high-fructose diet.
abstract: In rodents a high-fructose diet induces metabolic derangements similar to those in metabolic syndrome. Previously we suggested that in mouse liver an unidentified nuclear protein binding to the sterol regulatory element (SRE)-binding protein-1c (SREBP-1c) promoter region plays a key role for the response to high-fructose diet. Here, using MALDI-TOF MASS technique, we identified an X-chromosome-linked RNA binding motif protein (RBMX) as a new candidate molecule. In electrophoretic mobility shift assay, anti-RBMX antibody displaced the bands induced by fructose-feeding. Overexpression or suppression of RBMX on rat hepatoma cells regulated the SREBP-1c promoter activity. RBMX may control SREBP-1c expression in mouse liver in response to high-fructose diet.
authors.affiliation: Division of Molecular Genetics in Medicine, Department of Biochemistry and Molecular Biology, Shiga University of Medical Science, Shiga 520-2192, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Takemoto
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Nishio
authors.forename: Osamu
authors.initials: O
authors.lastname: Sekine
authors.forename: Chikako
authors.initials: C
authors.lastname: Ikeuchi
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Nagai
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Maeno
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Maegawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kimura
authors.forename: Atsunori
authors.initials: A
authors.lastname: Kashiwagi
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D045744:Cell Line, Tumor; D004032:Diet; D024202:Electrophoretic Mobility Shift Assay; D005632:Fructose / Q000008:administration & dosage*; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D011401:Promoter Regions, Genetic / Q000187:drug effects; D034622:RNA Interference; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics*
chemical_list: D000906:Antibodies; D034441:Heterogeneous-Nuclear Ribonucleoproteins; C518069:RBMX protein, mouse; C497388:Srebf1 protein, mouse; D051780:Sterol Regulatory Element Binding Protein 1; D005632:Fructose