The murine bronchopulmonary microcirculation in hapten-induced inflammation.
OBJECTIVE
The clinical observation of central bronchial artery hypertrophy in chronic lung inflammation suggests the possibility that the bronchial circulation may also participate in adaptive responses in peripheral lung inflammation.


METHODS
To investigate the potential role of the bronchial microcirculation in peripheral lung inflammation, we developed a murine model of lung inflammation using the intratracheal instillation of the peptide-hapten trinitrophenol in presensitized mice.


RESULTS
Clinical parameters indicated a peak inflammatory response at 96 hours. Similarly, gross and microscopic evidence of inflammation was observed 96 hours after antigen instillation. Using a forced oscillation technique to probe peripheral lung mechanics at 96 hours, we detected no change in central airway resistance (P > .05), but a significant increase in peripheral tissue resistance (P < .05). The structure of the bronchial circulation was investigated by microsphere occlusion of the pulmonary circulation and corrosion casting of the bronchial circulation. SEM of the bronchial artery casts demonstrated (1) the presence of the peripheral bronchial circulation in mice, (2) interconnections of the two systems in the distal bronchial arteries and at the level of alveolar capillaries, and (3) functional evidence of increased bronchial perfusion of alveolar capillaries during mononuclear inflammation.


CONCLUSIONS
These results suggest an important adaptive role of the bronchial circulation in pulmonary inflammation.
Ravnic
Dino J
DJ
Division of Thoracic Surgery, Brigham & Women's Hospital, Boston, Mass 02115, USA.
Konerding
Moritz A
MA
Pratt
Juan P
JP
Wolloscheck
Tanja
T
Huss
Harold T
HT
Mentzer
Steven J
SJ
D000818:Animals; D001980:Bronchi / Q000098:blood supply* / Q000473:pathology; D001981:Bronchial Arteries / Q000473:pathology; D016319:Corrosion Casting; D006241:Haptens; D008168:Lung / Q000503:physiopathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008833:Microcirculation; D008855:Microscopy, Electron, Scanning; D010851:Picrates; D011014:Pneumonia / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology*; D011650:Pulmonary Alveoli / Q000098:blood supply; D011652:Pulmonary Circulation
D006241:Haptens; D010851:Picrates; C005858:picric acid