A mouse model for assessing the impact of ingested nutrients on gastric emptying rate.
1. The nutrient content of meals can affect the rate of gastric emptying. The aim of the present study was to assess whether the gastric emptying breath test could detect nutrient-induced delays in gastric emptying. 2. Following ingestion of a non-nutrient, carbohydrate- or lipid-containing liquid, mice were placed into chambers and breath samples were collected at intervals. Analysis of the rate of (13)CO(2) excretion allowed the calculation of gastric half-excretion time. 3. Gastric half-excretion time was significantly delayed by the incorporation of carbohydrate or lipid into the test liquid. 4. The present study has shown that the breath test is sensitive enough to detect changes induced by altering the nutrient and caloric content of test meals.
Symonds
Erin L
EL
Centre for Paediatric and Adolescent Gastroenterology, Women's and Children's Hospital, North Adelaide, South Australia, Australia. erinsymonds@yahoo.com
Butler
Ross
R
Omari
Taher I
TI
D019342:Acetic Acid; D000818:Animals; D001944:Breath Tests; D002245:Carbon Dioxide / Q000378:metabolism; D002247:Carbon Isotopes; D005217:Fat Emulsions, Intravenous / Q000494:pharmacology; D005260:Female; D005502:Food; D005746:Gastric Emptying / Q000187:drug effects / Q000502:physiology*; D005947:Glucose / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D014867:Water / Q000494:pharmacology
D002247:Carbon Isotopes; D005217:Fat Emulsions, Intravenous; D014867:Water; D002245:Carbon Dioxide; D005947:Glucose; D019342:Acetic Acid