title: The C-terminus of alpha2-antiplasmin interacts with endothelial cells.
abstract: The serpin, alpha(2)-antiplasmin (alpha(2)AP), has an extended C-terminus relative to other inhibitors. This 51-residue region contains an RGD sequence; such sequences constitute a key recognition sequence for cell adhesion, mediated through integrins. In the present study, this sequence was expressed in Escherichia coli and its binding to endothelial cells and whether binding depends on the RGD sequence was investigated. Binding to the surface of human umbilical vein endothelial cells (HUVEC-C) was observed by flow cytometry and immunohistochemistry. Binding studies on immobilised cells showed specific and RGD-dependent binding of the peptides to HUVEC-C. The binding of the wild-type peptide to the HUVEC-C was significantly higher than that of a mutant peptide, in which RGD was replaced by SAA (P < 0.05, n = 4). Similarly, ethylenediaminetetraacetic acid decreased the binding of the wild-type peptide (P < 0.05, n = 4). The binding was competed out by full-length alpha(2)AP, fibronectin and anti-alpha(5)beta(1). This is the first evidence of binding of the C-terminus of alpha(2)AP to endothelial cells via its RGD sequence, with most but not all of the binding being integrin-mediated. We speculate that this interaction with alpha(2)AP may potentially play a role in the control of cellular fibrinolysis by regulating local plasmin activity on cell surfaces.
authors.affiliation: Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, UK. l.thomas@abdn.ac.uk
authors.forename: L
authors.initials: L
authors.lastname: Thomas
authors.forename: N R
authors.initials: NR
authors.lastname: Moore
authors.forename: S
authors.initials: S
authors.lastname: Miller
authors.forename: N A
authors.initials: NA
authors.lastname: Booth
mesh_terms: D001667:Binding, Competitive; D002448:Cell Adhesion; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism*; D004926:Escherichia coli; D005353:Fibronectins / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D039302:Integrin alphaVbeta3 / Q000378:metabolism; D008856:Microscopy, Fluorescence; D016297:Mutagenesis, Site-Directed; D009842:Oligopeptides / Q000378:metabolism*; D014471:Umbilical Veins; D000979:alpha-2-Antiplasmin / Q000378:metabolism*
chemical_list: D005353:Fibronectins; D039302:Integrin alphaVbeta3; D009842:Oligopeptides; D000979:alpha-2-Antiplasmin; C047981:arginyl-glycyl-aspartic acid