title: Abdominal aortic aneurysm is a specific antigen-driven T cell disease.
abstract: To determine whether monoclonal/oligoclonal T cells are present in abdominal aortic aneurysm (AAA) lesions, we amplified beta-chain T cell receptor (TCR) transcripts from these lesions by the nonpalindromic adaptor (NPA)-polymerase chain reaction (PCR)/V-beta-specific PCR followed by cloning and sequencing. Sequence analysis revealed the presence of substantial proportions of identical beta-chain TCR transcripts in AAA lesions in 9 of 10 patients examined, strongly suggesting the presence of oligoclonal populations of alphabeta TCR+ T cells. We have also shown the presence of oligoclonal populations of gammadelta TCR+ T cells in AAA lesions. Sequence analysis after appropriate PCR amplification and cloning revealed the presence of substantial proportions of identical VgammaI and VgammaII TCR transcripts in 15 of 15 patients examined, and of Vdelta1 and Vdelta2 TCR transcripts in 12 of 12 patients. These clonal expansions were very strong. All these clonal expansions were statistically significant by the binomial distribution. In other studies, we determined that mononuclear cells infiltrating AAA lesions express early- (CD69), intermediate- (CD25, CD38), and late- (CD45RO, HLA class II) activation antigens. These findings suggest that active ongoing inflammation is present in the aortic wall of patients with AAA. These results demonstrate that oligoclonal alphabeta TCR+ and gammadelta TCR+T cells are present in AAA lesions. These oligoclonal T cells have been clonally expanded in vivo in response to yet unidentified antigens. Although the antigenic specificity of these T cells remains to be determined, these T cells may play a significant role in the initiation and/or the propagation of the AAA. It appears that AAA is a specific antigen-driven T cell disease.
authors.affiliation: Department of Microbiology, Temple University School of Medicine, 3400 North Broad Street, Philadelphia, PA 19140, USA. Chris.Platsoucas@temple.edu
authors.forename: Chris D
authors.initials: CD
authors.lastname: Platsoucas
authors.forename: Song
authors.initials: S
authors.lastname: Lu
authors.forename: Ifeyinwa
authors.initials: I
authors.lastname: Nwaneshiudu
authors.forename: Charalambos
authors.initials: C
authors.lastname: Solomides
authors.forename: Alexis
authors.initials: A
authors.lastname: Agelan
authors.forename: Nektaria
authors.initials: N
authors.lastname: Ntaoula
authors.forename: Enkhtuya
authors.initials: E
authors.lastname: Purev
authors.forename: Li Ping
authors.initials: LP
authors.lastname: Li
authors.forename: Paschalis
authors.initials: P
authors.lastname: Kratsios
authors.forename: Efstratios
authors.initials: E
authors.lastname: Mylonas
authors.forename: Weon-Ju
authors.initials: WJ
authors.lastname: Jung
authors.forename: Kyle
authors.initials: K
authors.lastname: Evans
authors.forename: Sean
authors.initials: S
authors.lastname: Roberts
authors.forename: Yandi
authors.initials: Y
authors.lastname: Lu
authors.forename: Ricardo
authors.initials: R
authors.lastname: Layvi
authors.forename: Wan Lu
authors.initials: WL
authors.lastname: Lin
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zhang
authors.forename: John
authors.initials: J
authors.lastname: Gaughan
authors.forename: Dimitrios S
authors.initials: DS
authors.lastname: Monos
authors.forename: Emilia L
authors.initials: EL
authors.lastname: Oleszak
authors.forename: John V
authors.initials: JV
authors.lastname: White
mesh_terms: D000941:Antigens / Q000276:immunology*; D017544:Aortic Aneurysm, Abdominal / Q000150:complications* / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008206:Lymphatic Diseases / Q000150:complications* / Q000235:genetics / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D000941:Antigens; D011948:Receptors, Antigen, T-Cell