Association of human leukocyte antigen polymorphism with hepatitis B virus infection and genotypes.
Associations were studied between the polymorphism of northern Han Chinese leukocyte antigen (HLA) alleles and the outcomes of hepatitis B virus (HBV) infection and HBV genotypes. HLA-A, B, and DRB1 alleles in peripheral blood mononuclear cells (PBMCs) were detected by polymerase chain reaction (PCR) with sequence-specific primers. The PBMCs were collected from 61 persons who tested positive for hepatitis B surface antigen (HBsAg) for more than 6 months (Persistent group), 32 persons who tested negative for both HBsAg and HBV DNA but positive for both anti-HBc and anti-HBs (Recovered group), and 40 persons who tested negative for all serologic markers of HBV infection (Uninfected group). HBV genotypes in serum specimens from 56 of 61 patients with persistent HBV infection were determined by nested PCR with 6 pairs of HBV genotype-specific primers (A to F). The frequency of HLA-DRB1*12 was significantly higher in the Persistent group than in the Recovered group (P=0.004). HLA-A*02 was significantly higher in the Recovered group than in the Persistent group (P=0.044). HLA-DRB1*15 was significantly higher in the HBV genotype B group than in the C group (P=0.013). These findings suggested that there were associations not only between HLA polymorphisms and outcomes of HBV infection but also between HLA polymorphisms and the infected HBV genotypes.
Zhang
Shu-Yun
SY
Department of Microbiology, Harbin Medical University, Harbin, China.
Gu
Hong-Xi
HX
Li
Di
D
Yang
Shu-Fen
SF
Zhong
Zhao-Hua
ZH
Li
Xing-Ku
XK
Jin
Xi
X
D002681:China; D018450:Disease Progression; D006680:HLA Antigens / Q000097:blood / Q000145:classification / Q000235:genetics*; D015234:HLA-A Antigens / Q000097:blood / Q000235:genetics; D015235:HLA-B Antigens / Q000097:blood / Q000235:genetics; D006684:HLA-DR Antigens / Q000097:blood / Q000235:genetics; D059811:HLA-DRB1 Chains; D006509:Hepatitis B / Q000235:genetics* / Q000503:physiopathology* / Q000821:virology; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006515:Hepatitis B virus / Q000145:classification* / Q000235:genetics; D006801:Humans; D011110:Polymorphism, Genetic
D006680:HLA Antigens; D015234:HLA-A Antigens; D015235:HLA-B Antigens; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D006514:Hepatitis B Surface Antigens