Specificities of acetoxy derivatives of coumarins, biscoumarins, chromones, flavones, isoflavones and xanthones for acetoxy drug: protein transacetylase.
The earlier work carried out in our laboratory led to the identification of a novel rat liver microsomal enzyme termed as acetoxy drug: protein transacetylase (TAase), catalyzing the transfer of acetyl group from polyphenolic acetates (PA) to functional proteins. In this paper, we have reported the comparison of the specificities of acetoxy derivatives of coumarins, biscoumarins, chromones, flavones, isoflavones and xanthones with special reference to the phenyl moiety/bulky group on the pyran ring of PA. The results clearly indicated that compounds having phenyl moieties, when used as the substrates, resulted in a significant reduction of TAase catalyzed activity. The alteration in TAase catalyzed activation of NADPH cytochrome c reductase and inhibition of benzene-induced micronuclei in bone marrow cells by PA were in tune with their specificities to TAase.
Kumar
Ajit
A
Department of Biochemistry, V P Chest Institute, University of Delhi, Delhi 110 007, India.
Singh
Brajendra K
BK
Sharma
Nawal K
NK
Gyanda
Kapil
K
Jain
Sapan K
SK
Tyagi
Yogesh K
YK
Baghel
Anil S
AS
Pandey
Mukesh
M
Sharma
Sunil K
SK
Prasad
Ashok K
AK
Jain
Subhash C
SC
Rastogi
Ramesh C
RC
Raj
Hanumantharao G
HG
Watterson
Arthur C
AC
Van der Eycken
Erik
E
Parmar
Virinder S
VS
D000107:Acetylation; D000123:Acetyltransferases / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D002384:Catalysis; D002867:Chromones / Q000737:chemistry*; D003374:Coumarins / Q000737:chemistry*; D015195:Drug Design; D047309:Flavones / Q000737:chemistry*; D007529:Isoflavones / Q000737:chemistry*; D008099:Liver / Q000201:enzymology; D008297:Male; D015162:Micronucleus Tests; D008861:Microsomes / Q000201:enzymology; D015394:Molecular Structure; D011714:Pyrans / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D044004:Xanthones / Q000737:chemistry*
D002867:Chromones; D003374:Coumarins; D047309:Flavones; D007529:Isoflavones; D011714:Pyrans; D044004:Xanthones; D000123:Acetyltransferases