[Analysis of 37 drugs in whole blood by HPLC after solid phase extraction].
OBJECTIVE
To develop a specific, sensitive, reproducible SPE-HPLC method for the determination of 37 drugs in whole blood.


METHODS
With the doxapram as internal standard, Oasis column was used to extract drugs from whole blood. Two kinds of mobile phases were used in this study. Separations were achieved by a LiChrospher 100 RP-C18 (250 mm x 4.0 mm x 5 microm) column kept at 50 degrees C, the DAD detector was set at 230 nm and 250 nm.


RESULTS
The limit of detection were 1-30 ng/mL. The method showed excellent linearity and the linear correlation coefficient was > or =0.997 98. The relative standard deviation for between-day and within-day assay were <10%.


CONCLUSIONS
The method is effective, simple, reliable and has been used in real cases.
Liang
Chen
C
Shanghai Institute of Criminal Science and Technology, Shanghai 200083, China.
Zhang
Yu-Rong
YR
Jin
Qi-Yun
QY
Guo
You-Mei
YM
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004315:Doxapram / Q000097:blood / Q000302:isolation & purification; D004316:Doxepin / Q000097:blood / Q000302:isolation & purification; D004949:Estazolam / Q000097:blood / Q000302:isolation & purification; D005554:Forensic Medicine; D006801:Humans; D009020:Morphine / Q000097:blood / Q000302:isolation & purification; D010208:Papaverine / Q000097:blood / Q000302:isolation & purification; D004364:Pharmaceutical Preparations / Q000097:blood* / Q000302:isolation & purification; D011224:Prazosin / Q000097:blood* / Q000302:isolation & purification; D011343:Procaine / Q000097:blood / Q000302:isolation & purification; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*; D012997:Solvents / Q000737:chemistry
D004364:Pharmaceutical Preparations; D012997:Solvents; D004316:Doxepin; D004949:Estazolam; D011343:Procaine; D009020:Morphine; D004315:Doxapram; D010208:Papaverine; D011224:Prazosin