title: Differential response related to genotoxicity between eggplant (Solanum melanogena) skin aqueous extract and its main purified anthocyanin (delphinidin) in vivo.
abstract: Anthocyanins are the largest group of water-soluble pigments in the plant kingdom. A number of studies have demonstrated that anthocyanins present antioxidant capacity and show inhibitory effects on the growth of some cancer cells. Thus, the goal of this study was to evaluate both the antimutagenicity/antigenotoxicity and mutagenicity/genotoxicity of aqueous extract obtained from the Solanum melanogena, a possible novel source of anthocyanin, and its main purified anthocyanin extract (delphinidin), using the single cell (comet) assay and micronucleus test. Pretreatment with higher doses of the purified anthocyanin (10 and 20mg/kg b.w.) led to a statistically significant reduction (p<0.05) in the frequency of micronuclei in polychromatic erythrocytes induced by cyclophosphamide. The pattern of reduction ranged from 48% to 57% independent of concentration. No apparent genotoxicity and mutagenicity was found for either the anthocyanin or delphinidin extracts. Taken together, these results suggest that mice pre-treated with specific compounds present in anthocyanins (delphinidin) displayed a lower incidence of mutations induced by cyclophosphamide. This finding emphasizes the potential of natural colorants to prevent mutations and also the applicability of genotoxic evaluation for improving health. Furthermore, the results presented here could be an additional argument to support the use of anthocyanins in the diet.
authors.affiliation: Departamento de Nutrição, Escola de Farmácia e Odontologia de Alfenas, Centro Universitário Federal, MG, Brazil.
authors.forename: Luciana
authors.initials: L
authors.lastname: Azevedo
authors.forename: Patrícia L
authors.initials: PL
authors.lastname: Alves de Lima
authors.forename: José C
authors.initials: JC
authors.lastname: Gomes
authors.forename: Paulo C
authors.initials: PC
authors.lastname: Stringheta
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Ribeiro
authors.forename: Daisy M F
authors.initials: DM
authors.lastname: Salvadori
mesh_terms: D000818:Animals; D000872:Anthocyanins / Q000633:toxicity*; D000975:Antioxidants / Q000633:toxicity*; D020552:Comet Assay; D003520:Cyclophosphamide / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D009152:Mutagenicity Tests; D010936:Plant Extracts / Q000633:toxicity; D032323:Solanum melongena / Q000737:chemistry*
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D010936:Plant Extracts; D003520:Cyclophosphamide; C017185:delphinidin