Short-term cytotoxicity assessment of components of the epiphany resin-percha obturating system by indirect and direct contact millipore filter assays.
The Epiphany Resin-Percha Obturating System was assessed for cytotoxicity, compared with gutta-percha and AH-Plus sealer. Specimen disks (Resilon, gutta-percha), filled glass rings (sealers), or imbibed cellulose disks (primer, thinning resin) were placed over Millipore filters in direct or indirect contact with HeLa cell monolayer, incubated for 2 hours, and stained with tetrazolium blue. Cytotoxicity was rated by the surrounding unstained zone: none (0 mm), mild (</=7 mm), moderate (7-12 mm), or marked (>12 mm). Data were analyzed with one-way ANOVA and post hoc pairwise t tests. Unstained zones indicating moderate cytotoxicity were significantly larger (p < 0.05) for Epiphany primer than for thinning resin and for freshly mixed AH-Plus than for Epiphany sealer. Set sealers (24 and 48 hours), gutta-percha, and Resilon elicited noncytotoxic responses. In conclusion, cytotoxicity of set Epiphany sealer and Resilon was comparable with that of set AH-Plus and gutta-percha. Cytotoxicity of freshly mixed Epiphany sealer, primer, and thinning resin did not exceed that of freshly mixed AH-Plus.
Merdad
Khalid
K
Department of Endodontics, University of Toronto, Ontario, Canada.
Pascon
Alvaro Elizeu
AE
Kulkarni
Gajanan
G
Santerre
Paul
P
Friedman
Shimon
S
D000818:Animals; D002417:Cattle; D004853:Epoxy Resins / Q000633:toxicity; D006171:Gutta-Percha / Q000633:toxicity; D012387:Root Canal Filling Materials / Q000737:chemistry / Q000633:toxicity*; D012389:Root Canal Obturation / Q000379:methods*; D018675:Toxicity Tests / Q000379:methods
D004853:Epoxy Resins; D012387:Root Canal Filling Materials; C486893:epiphany sealer; C534916:epoxy resin-based root canal sealer; D006171:Gutta-Percha