title: Lack of association between proliferative verrucous leukoplakia and human papillomavirus infection.
abstract: OBJECTIVE
  To analyze proliferative verrucous leukoplakia (PVL) for the presence of human papillomavirus (HPV) in different stages of the disease.
  
  
  METHODS
  We studied 13 patients with PVL. In 10 patients (76.9%), a lesional biopsy was taken and frozen at -40 degrees C. Four patients were instructed to mouth rinse with sterile sera. The biopsy and rinse samples were analyzed for HPV by PCR.
  
  
  RESULTS
  We did not detect HPV infection in the PVL tissue or in the oral rinse of any of the 13 patients in any stage of the disease analyzed, neither in oral squamous cell carcinoma nor in the simple hyperkeratosis.
  
  
  CONCLUSIONS
  There was no association between PVL and HPV infection in our patients.
authors.affiliation: Oral Medicine, Valencia University, Valencia, Spain. bagan@uv.es
authors.forename: Jose V
authors.initials: JV
authors.lastname: Bagan
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Jimenez
authors.forename: Judith
authors.initials: J
authors.lastname: Murillo
authors.forename: Carmen
authors.initials: C
authors.lastname: Gavaldá
authors.forename: Rafael
authors.initials: R
authors.lastname: Poveda
authors.forename: Crispian
authors.initials: C
authors.lastname: Scully
authors.forename: Trinidad M
authors.initials: TM
authors.lastname: Alberola
authors.forename: Manuela
authors.initials: M
authors.lastname: Torres-Puente
authors.forename: Manuel
authors.initials: M
authors.lastname: Pérez-Alonso
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D052685:Alphapapillomavirus / Q000302:isolation & purification*; D001706:Biopsy; D002294:Carcinoma, Squamous Cell / Q000821:virology; D005260:Female; D006801:Humans; D007972:Leukoplakia, Oral / Q000821:virology*; D008875:Middle Aged; D009061:Mouth Mucosa / Q000821:virology; D009062:Mouth Neoplasms / Q000821:virology; D030361:Papillomavirus Infections / Q000821:virology*