Protective effect of Mangifera indica L. polyphenols on human T lymphocytes against activation-induced cell death.
Activation-induced cell death (AICD) plays an important role in maintenance of peripheral lymphocyte homeostasis. Reactive oxygen species (ROS) combined with simultaneous calcium (Ca(2+)) influx into the cytosol are required for induction of AICD. The extract obtained from the stem bark of Mangifera indica L. has shown to protect T cells from in vitro AICD. This extract is rich in polyphenolic compounds, the three main components of which are mangiferin (MA), catechin (C) and epicatechin (EC). The present study has focused on the possible contribution of the polyphenols MA, C and EC to the demonstrated protective effect of M. indica extract on in vitro human T cell AICD. Our results show that these polyphenols diminished the increase of intracellular ROS and free Ca(2+) induced by T cell receptor (TCR) triggering. In addition, these polyphenols attenuated AICD. Our findings suggest that the T cell survival effect of M. indica extract is mediated, at least in part, by its main polyphenols.
Hernandez
Patricia
P
Department of Biomedical Research, Center of Pharmaceutical Chemistry, P.O. Box 16042, Havana, Cuba. patricia.hdez@infomed.sld.cu
Rodriguez
Pedro C
PC
Delgado
Rene
R
Walczak
Henning
H
D017209:Apoptosis / Q000187:drug effects*; D017252:CD3 Complex / Q000276:immunology; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D031022:Mangifera / Q000737:chemistry*; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D059808:Polyphenols; D020011:Protective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism
D017252:CD3 Complex; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D020011:Protective Agents; D017382:Reactive Oxygen Species; D002118:Calcium