title: Extremely low frequency 7 Hz 100 microT electromagnetic radiation promotes differentiation in the human epithelial cell line HaCaT.
abstract: Electromagnetic therapy is a treatment method in which an electromagnetic or magnetic stimulus is used to achieve physiological changes in the body. The specific aim of the present work concerns the effectiveness of low frequency electromagnetic fields to modify the biochemical properties of human keratinocytes (HaCaT). Cells exposed to a 7 Hz 100 microT electromagnetic field for one hour (twice daily), indicated modification in shape and morphology. These modifications were also associated with different actin distribution as revealed by phalloidin fluorescence analysis. Indirect immunofluorescence with fluorescent antibodies against involucrin and beta-Catenin, both differentiation and adhesion markers, revealed an increase in involucrin and beta-Catenin expression, supporting the conclusion that exposure to electromagnetic field carries keratinocytes to an upper differentiation level. This study confirms our previous observation and supports the hypothesis that 7 Hz electromagnetic field, may modify cell biochemistry interfering in the differentiation and cellular adhesion of normal keratinocytes.
authors.affiliation: Istituto di Neurobiologia e Medicina Molecolare CNR, Rome, Italy. antonella.lisi@ims.rm.cnr.it
authors.forename: Antonella
authors.initials: A
authors.lastname: Lisi
authors.forename: Alberto
authors.initials: A
authors.lastname: Foletti
authors.forename: Mario
authors.initials: M
authors.lastname: Ledda
authors.forename: Emanuela
authors.initials: E
authors.lastname: Rosola
authors.forename: Livio
authors.initials: L
authors.lastname: Giuliani
authors.forename: Enrico
authors.initials: E
authors.lastname: D'Emilia
authors.forename: Settimio
authors.initials: S
authors.lastname: Grimaldi
mesh_terms: D000199:Actins / Q000378:metabolism; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003599:Cytoskeleton / Q000528:radiation effects; D004574:Electromagnetic Fields; D019084:Fluorescent Antibody Technique, Indirect; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D015603:Keratinocytes / Q000528:radiation effects*; D008855:Microscopy, Electron, Scanning; D010590:Phalloidine / Q000494:pharmacology; D011498:Protein Precursors / Q000096:biosynthesis; D013997:Time Factors; D051176:beta Catenin / Q000096:biosynthesis
chemical_list: D000199:Actins; D005456:Fluorescent Dyes; D011498:Protein Precursors; D051176:beta Catenin; D010590:Phalloidine; C032253:involucrin