Effects of edaravone on singlet oxygen released from activated human neutrophils.
The effects of edaravone, a curative agent for acute brain infarction, on singlet oxygen ((1)O2) released from activated human neutrophils were examined, and the effects were compared to those of histidine, a (1)O2 singlet oxygen scavenger. The neutrophils, stimulated with opsonized zymosan, released (1)O2 that was detected by chemiluminescence using a (1)O2 specific probe, trans-1-(2'-methoxyvinyl)pyrene. Edaravone dose-dependently suppressed the (1)O2 release with an IC(50) of approximately 0.3 microM, while the IC(50) of histidine was approximately 1 mM. This (1)O2 scavenging activity of edaravone might be involved in its curative effects on acute brain infarction.
Sommani
Piyanart
P
Institute of Advanced Energy, Kyoto University, Uji 611-0011, Japan.
Arai
Toshiyuki
T
Yamashita
Kouhei
K
Miyoshi
Takashi
T
Mori
Hiroko
H
Sasada
Masataka
M
Makino
Keisuke
K
D000328:Adult; D000983:Antipyrine / Q000031:analogs & derivatives* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D000077553:Edaravone; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006639:Histidine / Q000494:pharmacology; D006801:Humans; D008163:Luminescent Measurements; D018375:Neutrophil Activation; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D026082:Singlet Oxygen / Q000378:metabolism*
D016166:Free Radical Scavengers; D026082:Singlet Oxygen; D006639:Histidine; D000077553:Edaravone; D000983:Antipyrine