Rapid detection of Escherichia coli by using antibody-conjugated silver nanoshells.
Silver shells of 20 nm thickness have been deposited on silica particles of 200 nm diameter with narrow size distribution. Silver nanoshells dispersed in water exhibit a strong surface plasmon resonance band at 443 nm. This band was found to be very sensitive to rabbit immunoglobulin G antibodies, which were anchored on the nanoshells. These in turn could be utilized to detect the presence of small (approximately 5) to large numbers (approximately 10(9)) of Escherichia coli in water. The protocol presented here proves to be a specific, rapid, reliable, and inexpensive method to detect E. coli.
Kalele
Suchita A
SA
DST Unit on Nanoscience, University of Pune, Pune-411007, India. skk@physics.unipune.ernet.in
Kundu
Anita A
AA
Gosavi
Suresh W
SW
Deobagkar
Dileep N
DN
Deobagkar
Deepti D
DD
Kulkarni
Sulabha K
SK
D000936:Antigen-Antibody Complex / Q000032:analysis; D003199:Computer Systems; D003460:Crystallization / Q000379:methods; D004926:Escherichia coli / Q000276:immunology / Q000302:isolation & purification*; D029968:Escherichia coli Proteins / Q000276:immunology; D007118:Immunoassay / Q000379:methods*; D008422:Materials Testing; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D012834:Silver / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000379:methods*; D013499:Surface Properties
D000936:Antigen-Antibody Complex; D029968:Escherichia coli Proteins; D012834:Silver