Ultrastructure and gene mapping of the albino mutant al12 in rice (Oryza sativa L.).
Seedling albino mutation resistant to low temperature is an adaptability of rice (Oryza sativa L.) to cold. The mutant, a conditional expression controlled by development and temperature, differs from other albino mutants. The chlorophyll content of the mutant was measured using a portable chlorophyll meter, and the ultrastructure of the chloroplast was observed using a transmission electron microscope. Chlorophyll content was 1.2 SPAD, and the chloroplast did not develop, with only small vesicle-like structures. A segregation analysis of the reciprocal crosses between the albino mutation line with the rice line 9311 demonstrated that the albino trait was controlled by a single recessive gene, which was flanked by SSR markers RM5068 and RM3702 on the short arm of chromosome 8 with a distance of 0.5-1.1 cM and 4.9 cM, respectively. This gene was mapped within a 6 cM interval region and was tentatively referred to as al12.
Xia
Jiu-Cheng
JC
Rice Institute of Sichuan Agricultural University, Wenjiang 611130, China.
Wang
Yu-Ping
YP
Ma
Bing-Tian
BT
Yin
Zhao-Qing
ZQ
Hao
Ming
M
Kong
De-Wei
DW
Li
Shi-Gui
SG
D000220:Adaptation, Biological / Q000235:genetics; D002734:Chlorophyll / Q000378:metabolism; D002736:Chloroplasts / Q000648:ultrastructure; D002874:Chromosome Mapping; D003080:Cold Temperature; D017343:Genes, Plant; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000502:physiology / Q000648:ultrastructure*; D010641:Phenotype; D010858:Pigmentation / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D036226:Seedlings / Q000235:genetics / Q000502:physiology
D002734:Chlorophyll