[Quantitative analysis of epidermal growth factor receptor mRNA in laryngeal carcinoma by reverse transcription-real time polymerase chain reaction with TagMan probe].
OBJECTIVE
To establish a method of quantitative analysis of epidermal growth factor receptor(EGFR) mRNA in laryngeal carcinoma by reverse transcription-real time polymerase chain reaction with TagMan probe and evaluate its applicable value.


METHODS
The technique of reverse transcription-real time polymerase chain reaction with highly specific primers and probe was applied. And with GAPDH as internal standard, EGFR mRNA in 32 laryngeal carcinoma tissues, compared with their own macroscopically normal laryngeal mucosa tissues adjacent to the tumors, were quantitatively examined. The recombinant plasmid of EGFR was constructed, and then sequenced.


RESULTS
The recombinant plasmid of EGFR was successfully constructed. And the mRNA index of EGFR in 32 laryngeal carcinoma tissues in terms of M (QR) was 0.025 (0.076), which was higher than 0.008 (0.027) in their own macroscopically normal laryngeal mucosa tissues adjacent to the tumors, P value is 0. 007.


CONCLUSIONS
The method of reverse transcription-real time polymerase chain reaction with TagMan probe is quite objective, precise and high throughput in the application for the mensuration of EGFR mRNA in laryngeal carcinoma.
Chen
Jia
J
Department of Otorhinolaryngology, Second Affiliated Hospital of Medical College, Zhejiang University, Hangzhou 310009, China.
Yang
Bei-bei
BB
Zhang
Xing
X
Cao
Jiang
J
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics
D012333:RNA, Messenger; D066246:ErbB Receptors