Analysis of genetic elements regulating the methionine adenosyltransferase gene in Leishmania infantum.
Methionine adenosyltransferase (MAT) is an important enzyme for metabolic processes, inasmuch as its product, S-adenosylmethionine (AdoMet), plays a key role in trans-methylation, trans-sulphuration and polyamine synthesis. Our prior studies have shown that the Leishmania infantum genome contains two identical copies of the gene encoding MAT (MAT2 gene), arranged in head-to-tail configuration and alternating with another gene, called LORIEN that contains a zinc-finger motif. Both genes are constitutively expressed throughout the promastigote stage of the parasite cell cycle, and their flanking regions were detected by RT-PCR. Luciferase (luc) reporter assays indicated the presence of regulatory elements within the MAT2 3'UTR and intergenic region, and fragments responsible for such regulation were identified by deletional analysis. By site-directed mutagenesis of the wild-type -42 AG recognized in the trans-splicing of the MAT2 gene, the AG slightly downstream (position -36) was observed to be able to generate the same levels of luc expression, thus suggesting that potentially this gene has alternative spliced leader acceptor sites. The stability of MAT2 and LORIEN transcripts was very similar in both logarithmic and stationary phases. However, cycloheximide (CHX) inhibition of protein synthesis increased MAT2 and LORIEN mRNA levels in the logarithmic phase only, an indication that these genes are regulated in promastigotes at the post-transcriptional level by protein factors that targets both transcripts for degradation. However, during the stationary phase, another CHX-independent factor also led to MAT2 and LORIEN mRNAs degradation, indicating the existence of different mechanisms operating on the post-transcriptional regulation of these two genes.
García-Estrada
Carlos
C
Departamento de Farmacología y Toxicología, Universidad de León, Campus de Vegazana s/n, 24071 León, Spain.
Pérez-Pertejo
Yolanda
Y
Ordóñez
David
D
Balaña-Fouce
Rafael
R
Reguera
Rosa M
RM
D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D005786:Gene Expression Regulation; D017125:Genes, Protozoan; D018503:Genome, Protozoan; D018314:Leishmania infantum / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D008018:Life Cycle Stages; D008716:Methionine Adenosyltransferase / Q000235:genetics*; D005810:Multigene Family; D016297:Mutagenesis, Site-Directed; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D020871:RNA Stability / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016053:RNA, Protozoan / Q000235:genetics / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D020040:Trans-Splicing; D014158:Transcription, Genetic
D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D016053:RNA, Protozoan; D003513:Cycloheximide; D008716:Methionine Adenosyltransferase