The end1 gene of Schizosaccharomyces pombe coding for a DNase is identical with the pnu1 gene coding for an RNase.
The DNA nuclease activity encoded by the end1 gene, and its inactivation by mutation, was described in connection with the characterization of DNA topoisomerases in the fission yeast Schizosaccharomyces pombe (Uemura and Yanagida, 1984). Subsequently, end1 mutant strains were used for the preparation of cell extracts for the study of enzymes and intermediates involved in DNA metabolism. The molecular identification of the end1 gene and its identity with the pnu1 gene is presented. The end1-458 mutation alters glycine to glutamate in the conserved motif TGPYLP. The pnu1 gene codes for an RNase that is induced by nitrogen starvation (Nakashima et al., 2002b). Thus, the End1/Pnu1 protein, like related mitochondrial proteins in other organisms, is an example of a sugar-non-specific nuclease. The analysis of strains carrying a pnu1 deletion revealed no defects in meiotic recombination and spore viability.
Sakem
Benjamin
B
Institute of Cell Biology, University of Bern, CH-3012 Bern, Switzerland.
Kohli
Jürg
J
D000595:Amino Acid Sequence; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D003851:Deoxyribonucleases / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D012260:Ribonucleases / Q000235:genetics*; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000235:genetics*; D016415:Sequence Alignment
D004271:DNA, Fungal; D003851:Deoxyribonucleases; D012260:Ribonucleases