title: Prevention of mammary tumorigenesis by intermittent caloric restriction: does caloric intake during refeeding modulate the response?
abstract: Chronic caloric restriction (CCR) prevents mammary tumorigenesis in rodents, but a protective effect for intermittent caloric restriction (ICR) is less well documented. We recently reported that ICR reduced mammary tumor (MT) incidence of mouse mammary tumor virus-transforming growth factor (MMTV-TGF)-alpha mice to a greater extent than did CCR. Here, we repeated this protocol and obtained serum and tissue samples. Ad libitum (AL) MMTV-TGF-alpha mice were fed AIN-93M diet. Beginning at 10 weeks of age, ICR mice received isocaloric AIN-93M-mod diet (2-fold increases in protein, fat, vitamins, and minerals) at 50% of ad libitum for 3 weeks followed by 3 weeks refeeding with AIN-93M diet. CCR mice were pair-fed AIN-93M:AIN-93M-mod (2:1) matching intakes for restriction/refeeding cycles. Mice were sacrificed for MT size, at 79 (end of 12th restriction) or at 80 (1 week after 12th refeeding) weeks of age. AL and ICR-80 mice had heavier body weights than ICR-79 and CCR mice (P < 0.0001). Cumulative food intakes of ICR and CCR mice were reduced 12% and 15% versus AL mice (P < 0.0001). However, ICR mice consumed significantly (P < 0.0001) more food than did AL mice during refeeding. MT incidence was 84%, 13%, and 27% for AL, ICR, and CCR mice, respectively. MT weight (P < 0.0011) and number (P < 0.01) were higher for AL mice compared with ICR and CCR mice. AL and ICR-80 mice had similar serum IGF-I levels, but only AL values were higher than those of ICR-79 and CCR mice (P < 0.0017). ICR mice had more MT DNA breaks compared with AL and CCR mice, suggesting enhanced apoptosis (P < 0.02). AL mice had higher mammary fat pad ObR and ObRb leptin receptor mRNA expression than did ICR and CCR mice (P < 0.001), but there was no effect on MTs. These results confirm that ICR prevents development of MTs to a greater extent than does CCR, although "overeating" during refeeding may compromise this protection.
authors.affiliation: Hormel Institute, University of Minnesota, Austin, MN 55912, USA. mpcleary@hi.umn.edu
authors.forename: Margot P
authors.initials: MP
authors.lastname: Cleary
authors.forename: Xin
authors.initials: X
authors.lastname: Hu
authors.forename: Michael E
authors.initials: ME
authors.lastname: Grossmann
authors.forename: Subhash C
authors.initials: SC
authors.lastname: Juneja
authors.forename: Soner
authors.initials: S
authors.lastname: Dogan
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Grande
authors.forename: Nita J
authors.initials: NJ
authors.lastname: Maihle
mesh_terms: D000818:Animals; D001835:Body Weight; D031204:Caloric Restriction; D053960:DNA Breaks; D004032:Diet; D002149:Energy Intake; D005260:Female; D015870:Gene Expression; D015994:Incidence; D007334:Insulin-Like Growth Factor I / Q000032:analysis; D015674:Mammary Neoplasms, Animal / Q000473:pathology / Q000517:prevention & control*; D051379:Mice; D008822:Mice, Transgenic; D012333:RNA, Messenger / Q000032:analysis; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000235:genetics; D054411:Receptors, Leptin; D016211:Transforming Growth Factor alpha / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; D016211:Transforming Growth Factor alpha; D007334:Insulin-Like Growth Factor I