title: Genetic stock identification of chum salmon in the Bering Sea and North Pacific Ocean using mitochondrial DNA microarray.
abstract: A newly developed DNA microarray was applied to identify mitochondrial (mt) DNA haplotypes of more than 2200 chum salmon in the Bering Sea and North Pacific Ocean in September 2002 and also 2003, when the majority of maturing fish were migrating toward their natal river. The distribution of haplotypes occurring in Asian and North American fish in the surveyed area was similar in the 2 years. A conditional maximum likelihood method for estimation of stock compositions indicated that the Japanese stocks were distributed mainly in the north central Bering Sea, whereas the Russian stocks were mainly in the western Bering Sea. The North American stocks were abundant in the North Pacific Ocean around the Aleutian Islands. These results indicate that the Asian and North American stocks of chum salmon are nonrandomly distributed in the Bering Sea and the North Pacific Ocean, and further the oligonuleotide DNA microarray developed by us has a high potential for identification of stocks among mixed ocean aggregates of high-seas chum salmon.
authors.affiliation: Research and Development Center, Nisshinbo Industries, Inc., 1-2-3, Onodai, Midori-ku, Chiba, 267-0056, Japan. moriya@nisshinbo.co.jp
authors.forename: Shogo
authors.initials: S
authors.lastname: Moriya
authors.forename: Shunpei
authors.initials: S
authors.lastname: Sato
authors.forename: Tomonori
authors.initials: T
authors.lastname: Azumaya
authors.forename: Osamu
authors.initials: O
authors.lastname: Suzuki
authors.forename: Shigehiko
authors.initials: S
authors.lastname: Urawa
authors.forename: Akihisa
authors.initials: A
authors.lastname: Urano
authors.forename: Syuiti
authors.initials: S
authors.lastname: Abe
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000737:chemistry; D004272:DNA, Mitochondrial / Q000235:genetics*; D003710:Demography; D014644:Genetic Variation; D005828:Genetics, Population; D005843:Geography; D006239:Haplotypes; D016013:Likelihood Functions; D020411:Oligonucleotide Array Sequence Analysis / Q000662:veterinary*; D017685:Oncorhynchus keta / Q000145:classification / Q000235:genetics*; D010140:Pacific Ocean; D016133:Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial