Ancient noncoding elements conserved in the human genome.
Cartilaginous fishes represent the living group of jawed vertebrates that diverged from the common ancestor of human and teleost fish lineages about 530 million years ago. We generated approximately 1.4x genome sequence coverage for a cartilaginous fish, the elephant shark (Callorhinchus milii), and compared this genome with the human genome to identify conserved noncoding elements (CNEs). The elephant shark sequence revealed twice as many CNEs as were identified by whole-genome comparisons between teleost fishes and human. The ancient vertebrate-specific CNEs in the elephant shark and human genomes are likely to play key regulatory roles in vertebrate gene expression.
Venkatesh
Byrappa
B
Institute of Molecular and Cell Biology, 61 Biopolis Drive, Singapore 138673. mcbbv@imcb.a-star.edu.sg
Kirkness
Ewen F
EF
Loh
Yong-Hwee
YH
Halpern
Aaron L
AL
Lee
Alison P
AP
Johnson
Justin
J
Dandona
Nidhi
N
Viswanathan
Lakshmi D
LD
Tay
Alice
A
Venter
J Craig
JC
Strausberg
Robert L
RL
Brenner
Sydney
S
D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D021901:DNA, Intergenic; D004742:Enhancer Elements, Genetic; D019143:Evolution, Molecular; D016678:Genome; D015894:Genome, Human; D006801:Humans; D008969:Molecular Sequence Data; D012045:Regulatory Sequences, Nucleic Acid; D012754:Sharks / Q000235:genetics*; D023741:Takifugu / Q000235:genetics; D015027:Zebrafish / Q000235:genetics
D021901:DNA, Intergenic