title: Protein expression profiling of the shrimp cellular response to white spot syndrome virus infection.
abstract: To better understand the pathogenesis of white spot syndrome virus (WSSV) and to determine which cell pathways might be affected after WSSV infection, two-dimensional gel electrophoresis (2-DE) was used to produce protein expression profiles from samples taken at 48 h post-infection (hpi) from the stomachs of Litopenaeus vannamei (also called Penaeus vannamei) that were either specific pathogen free or else infected with WSSV. Seventy-five protein spots that consistently showed either a marked change (>50%) in accumulated levels or else were highly expressed throughout the course of WSSV infection were selected for further study. After in-gel trypsin digestion followed by LC-nanoESI-MS/MS, bioinformatics databases were searched for matches. A total of 53 proteins were identified, with functions that included energy production, calcium homeostasis, nucleic acid synthesis, signaling/communication, oxygen carrier/transportation, and SUMO-related modification. 2-DE results were shown to be consistent with relative EST database data from a previously developed EST database of two Penaeus monodon cDNA libraries. For seven selected genes, 2-DE and EST data were also compared with transcriptional time-course RT-PCR data. This study is the first global analysis of differentially expressed proteins in WSSV-infected shrimp, and in addition to increasing our understanding of the molecular pathogenesis of this virus-associated shrimp disease, the results presented here should be useful both for identifying potential biomarkers and for developing antiviral measures.
authors.affiliation: Institute of Biochemical Sciences, National Taiwan University, Taipei 106, Taiwan, ROC.
authors.forename: Hao-Ching
authors.initials: HC
authors.lastname: Wang
authors.forename: Han-Ching
authors.initials: HC
authors.lastname: Wang
authors.forename: Jiann-Horng
authors.initials: JH
authors.lastname: Leu
authors.forename: Guang-Hsiung
authors.initials: GH
authors.lastname: Kou
authors.forename: Andrew H-J
authors.initials: AH
authors.lastname: Wang
authors.forename: Chu-Fang
authors.initials: CF
authors.lastname: Lo
mesh_terms: D000818:Animals; D004266:DNA Virus Infections / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D015180:Electrophoresis, Gel, Two-Dimensional; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D033561:Penaeidae / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D040901:Proteomics / Q000379:methods*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013047:Specific Pathogen-Free Organisms; D046848:White spot syndrome virus 1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger