Enhanced biodegradation of iopromide and trimethoprim in nitrifying activated sludge.
Iopromide (an X-ray contrast agent) and trimethoprim (an antibacterial drug) are frequently detected pharmaceuticals in effluents of wastewater treatment plants (WWTPs) and in surface waters due to their persistence and high usage. Laboratory-scale experiments showed that a significantly higher removal rate in nitrifying activated sludge as compared to conventional activated sludge was observed for both iopromide and trimethoprim. When the activity of the nitrifying bacteria was inhibited, the percent removal of iopromide decreased from 97 to 86% while trimethoprim removal decreased from 70 to 25%. The metabolite of iopromide identified when nitrification was not inhibited was a dehydroxylated iopromide at the two side chains. However, when the nitrifying bacteria were inhibited the metabolite identified was a carboxylate, formed during the oxidation of the primary alcohol on the side chain of iopromide. These results suggest that the nitrifying bacteria are important in the observed biodegradation of iopromide in the activated sludge with higher solid retention time (SRT). Results from the laboratory-scale study were corroborated by the observed removal efficiencies in a full-scale municipal WWTP, which showed that iopromide (ranging from 0.10 to 0.27 microg/L) and trimethoprim (ranging from 0.0.08 to 0.53 microg/L) were removed more effectively in the nitrifying activate sludge which has a higher SRT (49 days) than in the conventional activated sludge (SRT of 6 days). In nitrifying activated sludge, the percent removal of iopromide in the WWTP reached 61%, while in conventional activated sludge, average removal was negligible. For trimethoprim, removal was limited to about 1% in the conventional activated sludge, while in the nitrifying activated sludge, the removal was increased to 50%.
Batt
Angela L
AL
Department of Chemistry, The State University of New York at Buffalo, 608 Natural Sciences Complex, Buffalo, New York 14260-3000, USA.
Kim
Sungpyo
S
Aga
Diana S
DS
D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002853:Chromatography, Liquid; D007472:Iohexol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D012722:Sewage / Q000032:analysis* / Q000382:microbiology; D053719:Tandem Mass Spectrometry; D014295:Trimethoprim / Q000737:chemistry / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D012722:Sewage; D014874:Water Pollutants, Chemical; D007472:Iohexol; C038192:iopromide; D014295:Trimethoprim