Genetically selected stem cells from human adipose tissue express cardiac markers.
In the present study, the potential of human adipose-derived stem cells to differentiate into cells with characteristics of cardiomyocytes was investigated. Adipose tissue-derived stem cells (ADSCs) were transduced with two different lentiviral vectors simultaneously: (1) a lentiviral vector expressing eGFP controlled by the Nkx2.5 promoter and (2) a lentiviral vector expressing DsRed2 controlled by the myosin light chain-2v promoter (MLC-2v). Nkx2.5-eGFP and MLC-2v-DsRed2 dual positive cells were isolated by FACS. Immunostaining and RT-PCR analysis of the dual positive cells revealed that these cells are positive for Nkx2.5, cardiac troponin I, and L-type calcium channel alpha-1c subunit. Electrophysiology studies demonstrated the presence of functional voltage-dependent calcium and potassium channels. These observations confirm that cardiac progenitor cells can be isolated and enriched from human adipose-derived stem cells using lentiviral selection, and they might represent a new source for cell therapy for myocardial infarction and heart failure.
Bai
Xiaowen
X
Department of Molecular Pathology, University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA.
Pinkernell
Kai
K
Song
Yao-Hua
YH
Nabzdyk
Christoph
C
Reiser
Jakob
J
Alt
Eckhard
E
D000273:Adipose Tissue / Q000166:cytology*; D020746:Calcium Channels, L-Type / Q000502:physiology; D002454:Cell Differentiation; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000096:biosynthesis; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D008164:Luminescent Proteins / Q000096:biosynthesis; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism; D024642:Potassium Channels, Voltage-Gated / Q000502:physiology; D013234:Stem Cells / Q000378:metabolism*; D014161:Transduction, Genetic
D020746:Calcium Channels, L-Type; D008164:Luminescent Proteins; D024642:Potassium Channels, Voltage-Gated; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins