Myd88-dependent positioning of Ptgs2-expressing stromal cells maintains colonic epithelial proliferation during injury.
We identified cellular and molecular mechanisms within the stem cell niche that control the activity of colonic epithelial progenitors (ColEPs) during injury. Here, we show that while WT mice maintained ColEP proliferation in the rectum following injury with dextran sodium sulfate, similarly treated Myd88(-/-) (TLR signaling-deficient) and prostaglandin-endoperoxide synthase 2(-/-) (Ptgs2(-/-)) mice exhibited a profound inhibition of epithelial proliferation and cellular organization within rectal crypts. Exogenous addition of 16,16-dimethyl PGE(2) (dmPGE(2)) rescued the effects of this injury in both knockout mouse strains, indicating that Myd88 signaling is upstream of Ptgs2 and PGE(2). In WT and Myd88(-/-) mice, Ptgs2 was expressed in scattered mesenchymal cells. Surprisingly, Ptgs2 expression was not regulated by injury. Rather, in WT mice, the combination of injury and Myd88 signaling led to the repositioning of a subset of the Ptgs2-expressing stromal cells from the mesenchyme surrounding the middle and upper crypts to an area surrounding the crypt base adjacent to ColEPs. These findings demonstrate that Myd88 and prostaglandin signaling pathways interact to preserve epithelial proliferation during injury using what we believe to be a previously undescribed mechanism requiring proper cellular mobilization within the crypt niche.
Brown
Sarah L
SL
Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Riehl
Terrence E
TE
Walker
Monica R
MR
Geske
Michael J
MJ
Doherty
Jason M
JM
Stenson
William F
WF
Stappenbeck
Thaddeus S
TS
D000818:Animals; D002455:Cell Division; D051546:Cyclooxygenase 2 / Q000172:deficiency / Q000235:genetics*; D007413:Intestinal Mucosa / Q000473:pathology* / Q000503:physiopathology; D051379:Mice; D018345:Mice, Knockout; D008957:Models, Genetic; D053594:Myeloid Differentiation Factor 88 / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000201:enzymology / Q000502:physiology*; D014158:Transcription, Genetic; D014947:Wounds and Injuries / Q000503:physiopathology
C507780:Myd88 protein, mouse; D053594:Myeloid Differentiation Factor 88; D051546:Cyclooxygenase 2; C489691:Ptgs2 protein, rat