title: Deficits in synaptic transmission and learning in amyloid precursor protein (APP) transgenic mice require C-terminal cleavage of APP.
abstract: Synaptic dysfunction has been shown to be one of the earliest correlates of disease progression in animal models of Alzheimer's disease. Amyloid-beta protein (Abeta) is thought to play an important role in disease-related synaptic dysfunction, but the mechanism by which Abeta leads to synaptic dysfunction is not understood. Here we describe evidence that cleavage of APP in the C terminus may be necessary for the deficits present in APP transgenic mice. In APP transgenic mice with a mutated cleavage site at amino acid 664, normal synaptic transmission, synaptic plasticity, and learning were maintained despite the presence of elevated levels of APP, Abeta42, and even plaque accumulation. These results indicate that cleavage of APP may play a critical role in the development of synaptic and behavioral dysfunction in APP transgenic mice.
authors.affiliation: Salk Institute for Biological Studies, La Jolla, California 92037, USA. saganich@salk.edu
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Saganich
authors.forename: Brock E
authors.initials: BE
authors.lastname: Schroeder
authors.forename: Veronica
authors.initials: V
authors.lastname: Galvan
authors.forename: Dale E
authors.initials: DE
authors.lastname: Bredesen
authors.forename: Edward H
authors.initials: EH
authors.lastname: Koo
authors.forename: Stephen F
authors.initials: SF
authors.lastname: Heinemann
mesh_terms: D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D016564:Amyloid beta-Protein Precursor / Q000172:deficiency* / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D019706:Excitatory Postsynaptic Potentials / Q000235:genetics; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007858:Learning / Q000502:physiology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D016297:Mutagenesis, Site-Directed; D009473:Neuronal Plasticity / Q000235:genetics; D010446:Peptide Fragments / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D009435:Synaptic Transmission / Q000235:genetics*
chemical_list: D016564:Amyloid beta-Protein Precursor; D010446:Peptide Fragments