Valproate and delta-sleep peptide display high efficacy against metaphit-induced audiogenic seizure in rats.
The effects of valproate (VPA) and delta sleep-inducing peptide (DSIP) on metaphit-induced generalized, audiogenic seizure in adult rat males were compared. The animals were i.p. injected with: (1) Saline; (2) metaphit (mp, 10 mg kg(-1)); 3. metaphit (10 mg kg(-1)) and 8 h later with DSIP (0.1, 0.2, 0.4 or 1.0 mg kg(-1)), 4. metaphit (10 mg kg(-1)) and 8 h later with VPA (50, 75 or 100 mg kg(-1)); 5. DSIP alone (1.0 mg kg(-1)) and 6. VPA, alone (100 mg kg(-1)). The rats were exposed to sound stimulation at hourly intervals and the behavior and EEG were analyzed. The EEG signals in metaphit rats appeared as a sleep-like pattern and spike-wave complexes with increased power spectra. Valproate and DSIP reduced the incidence of seizure and prolonged duration of latency in a dose-dependent manner. ED50 of valproate in the 1st hour after administration was 63.19 mg kg(-1) and that of DSIP 3.19 mg kg(-1) four hours after injection. This suggests that VPA, reached a peak of action immediately after the application, while DSIP had a prolonged action, mildly reducing, but not abolishing metaphit seizure. None of the applied VPA and DSIP doses eliminated the metaphit-provoked EEG signs of epileptiform activity.
Stanojlović
O
O
Department of Physiology, School of Medicine, University of Belgrade, Visegradska 26/II, 11000 Belgrade, Serbia maxol@eunet.yu
Hrncić
D
D
Zivanović
D
D
Rasić
A
A
Susić
V
V
D000818:Animals; D000927:Anticonvulsants / Q000627:therapeutic use*; D003701:Delta Sleep-Inducing Peptide / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D004569:Electroencephalography / Q000187:drug effects; D020195:Epilepsy, Reflex / Q000139:chemically induced / Q000517:prevention & control*; D008297:Male; D010622:Phencyclidine / Q000031:analogs & derivatives; D051381:Rats; D017208:Rats, Wistar; D014635:Valproic Acid / Q000627:therapeutic use*
D000927:Anticonvulsants; D014635:Valproic Acid; D003701:Delta Sleep-Inducing Peptide; C044705:metaphit; D010622:Phencyclidine