Edelfosine protects precultured heart fragments against the invasion of malignant cells through altered sialylation.
1-O-octadecyl-2-O-methylglycero-3-phosphocholine (ET-18-OMe)-treated precultured heart fragments (PHF) are resistant to the invasion of malignant cells. Previous studies have demonstrated that this effect is due to the alterations of the N-linked glycoproteins in PHF after 48-h ET-18-OMe treatment. Moreover, the observed effect was still present seven days after ET-18-OMe was omitted. The present study reveals that approximately 13.4% of the administered ET-18-OMe was taken up by PHF and about 75% of the initial uptake was still present after ET-18-OMe was removed. In addition, we found significant changes in the sialic acid content and sialyltransferase activities in both conditions. Overall, these results clearly demonstrate that the uptake and retention of ET-18-OMe are responsible for the resistance to the invasion of malignant cells due to the altered sialylation of the cell surface glycoproteins in PHF.
Van Slambrouck
S
S
Department of Chemistry, Laboratory of Biochemical and Biomedical Research, New Mexico Tech, Socorro, NM 87801, USA, and Laboratory of Experimental Cancerology, Department of Radiotherapy and Nuclear Medicine, University Hospital, Ghent, Belgium.
Steelant
W F A
WF
Lopez
M
M
Recchi
M A
MA
Boilly-Marer
Y
Y
Bruyneel
E A
EA
Delannoy
P
P
Verbert
A
A
Van Den Eeckhout
E G
EG
Deforce
D
D
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D019921:Biotinylation; D015153:Blotting, Western; D002462:Cell Membrane / Q000378:metabolism; D002642:Chick Embryo; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D006023:Glycoproteins / Q000378:metabolism; D008956:Models, Chemical; D009206:Myocardium / Q000473:pathology*; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D009361:Neoplasm Invasiveness; D010742:Phospholipid Ethers / Q000494:pharmacology*; D012794:Sialic Acids / Q000378:metabolism*; D012799:Sialyltransferases / Q000378:metabolism
D000970:Antineoplastic Agents; D006023:Glycoproteins; D010742:Phospholipid Ethers; D012794:Sialic Acids; C026659:edelfosine; D012799:Sialyltransferases; D019158:N-Acetylneuraminic Acid