[IL-10 inhibits inducible expression pattern of beta-defensin-2 in human peripheral blood cells].
To investigate the effect of IL-10 on the inducibility of human beta-defensin 2 (hBD-2) in human peripheral blood cells.



Peripheral blood samples were collected from 22 healthy individuals and co-cultured with 0 ng/ml lipopolysaccharide (LPS), 100 ng/ml LPS, 10 ng/ml IL-10, or 100 ng/ml LPS plus 10 ng/ml IL-10 at 37 degree for 6 h. Total RNA was extracted from peripheral blood cells and the mRNA level of hBD-2 was detected by relative quantitative real-time PCR..



No detectable level of hBD-2 mRNA was found in normal peripheral blood cells with stimulation of 0 ng/ml LPS or 10 ng/ml IL-10. The mRNA level of hBD-2 was increased to 166.9 +/- 35.14 after 100 ng/ml LPS challenge, while the mRNA level of hBD-2 was decreased to 30.40 +/- 9.18 after the co-stimulation of 100 ng/ml LPS plus 10 ng/ml IL-10; which was significantly lower than that with LPS alone (P<0.05).



The expression of hBD-2 gene can be induced by LPS.IL-10 inhibits the inducible expression of hBD-2.
Chen
Qi-xing
QX
Department of Anaesthesiology, The First AffiliatedHospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
Zhou
Jing-lin
JL
Book
Malte
M
D001773:Blood Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D006801:Humans; D016753:Interleukin-10 / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D023083:beta-Defensins / Q000235:genetics*
C106641:DEFB4A protein, human; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D023083:beta-Defensins; D016753:Interleukin-10