SILENCE: a new forward genetic technology.
Silencing induced by long terminal repeat (LTR)-encoded cis-acting response element, termed SILENCE, is a forward genetic system that allows for conditional, epigenetic control of host-gene transcription. This new research tool is independent of gene mutation or disruption, does not require complementation, and conditional gene repression appears complete at the level of protein function. SILENCE functions in hypodiploid cells and is a platform technology with broad applications in gene discovery.
Banks
David J
DJ
Department of Microbiology, Immunology and Molecular Genetics, 609 Charles E. Young Dr. East, Molecular Sciences Building, University of California at Los Angeles (UCLA), Los Angeles, California 90095, USA.
Bradley
Kenneth A
KA
D000818:Animals; D000942:Antigens, Bacterial / Q000494:pharmacology; D001427:Bacterial Toxins / Q000494:pharmacology; D016466:CHO Cells; D002999:Clone Cells; D006224:Cricetinae; D003412:Cricetulus; D020868:Gene Silencing; D005821:Genetic Techniques; D009154:Mutation; D010641:Phenotype; D018000:Receptors, Peptide / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D013329:Structure-Activity Relationship; D020079:Terminal Repeat Sequences / Q000235:genetics
D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D018000:Receptors, Peptide; C030325:anthrax toxin; C070309:anthrax toxin receptors