Selective ultratrace detection of Al(III) and Ga(III) complexed with a calcein isomer by capillary zone electrophoresis with laser-induced fluorescence detection. Comparison of emissive polyaminocarboxylates as derivatizing ligands.
A highly sensitive and selective capillary zone electrophoresis with laser-induced fluorescence detection (CZE-LIF) for Al(3+) and Ga(3+) was developed using a calcein 4',5'-isomer as a pre-capillary complexing agent. Direct fluorescent detection without an agent added to the carrier buffer provided the notable detection limits of 3.9x10(-10) and 4.6x10(-10)moldm(-3) for Al and Ga, respectively. By applying this system successfully to a river water sample, its potential for practical applications was ascertained. A comparison of three aromatic polyaminocarboxylate ligands related to the coordination structure and the distance between the fluorophore and the chelating moiety, revealed some interesting factors regarding their selectivity.
Saito
Shingo
S
Department of Applied and Environmental Chemistry, Kitami Institute of Technology, Kitami, Hokkaido 090-8507, Japan. shingo@chem.kitami-it.ac.jp
Shimidzu
Jun-Ichi
J
Yoshimoto
Keitaro
K
Maeda
Mizuo
M
Aoyama
Masakazu
M
D000535:Aluminum / Q000032:analysis* / Q000737:chemistry; D002118:Calcium / Q000737:chemistry; D002219:Carbamates / Q000737:chemistry*; D002614:Chelating Agents / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D005452:Fluoresceins / Q000737:chemistry*; D005453:Fluorescence; D005618:Fresh Water / Q000737:chemistry; D005708:Gallium / Q000032:analysis* / Q000737:chemistry; D007834:Lasers; D008024:Ligands; D012680:Sensitivity and Specificity; D014874:Water Pollutants, Chemical / Q000032:analysis
C518187:4',5'-bis((bis(carboxymethyl)amino)methyl)fluorescein; D002219:Carbamates; D002614:Chelating Agents; D005452:Fluoresceins; D008024:Ligands; D014874:Water Pollutants, Chemical; D005708:Gallium; D000535:Aluminum; D002118:Calcium