title: Mechano-transduction of DNA hybridization and dopamine oxidation through electrodeposited chitosan network.
abstract: While microcantilevers offer exciting opportunities for mechano-detection, they often suffer from limitations in either sensitivity or selectivity. To address these limitations, we electrodeposited a chitosan film onto a cantilever surface and mechano-transduced detection events through the chitosan network. Our first demonstration was the detection of nucleic acid hybridization. In this instance, we electrodeposited the chitosan film onto the cantilever, biofunctionalized the film with oligonucleotide probe, and detected target DNA hybridization by cantilever bending in solution (static mode) or resonant frequency shifts in air (dynamic mode). In both detection modes, we observed a two-order of magnitude increase in sensitivity compared to values reported in literature for DNA immobilized on self-assembled monolayers. In our second demonstration, we coupled electrochemical and mechanical modes to selectively detect the neurotransmitter dopamine. A chitosan-coated cantilever was biased to electrochemically oxidize dopamine solution. Dopamine's oxidation products react with the chitosan film and create a tensile stress of approximately 1.7 MPa, causing substantial cantilever bending. A control experiment was performed with ascorbic acid solution. It was shown that the electrochemical oxidation of ascorbic acid does not lead to reactions with chitosan and does not change cantilever bending. These results suggest that chitosan can confer increased sensitivity and selectivity to microcantilever sensors.
authors.affiliation: Department of Electrical and Computer Engineering, University of Maryland, College Park, MD 20742, USA.
authors.forename: Stephan T
authors.initials: ST
authors.lastname: Koev
authors.forename: Michael A
authors.initials: MA
authors.lastname: Powers
authors.forename: Hyunmin
authors.initials: H
authors.lastname: Yi
authors.forename: Li-Qun
authors.initials: LQ
authors.lastname: Wu
authors.forename: William E
authors.initials: WE
authors.lastname: Bentley
authors.forename: Gary W
authors.initials: GW
authors.lastname: Rubloff
authors.forename: Gregory F
authors.initials: GF
authors.lastname: Payne
authors.forename: Reza
authors.initials: R
authors.lastname: Ghodssi
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D048271:Chitosan / Q000737:chemistry*; D003430:Cross-Sectional Studies; D004247:DNA / Q000737:chemistry*; D004298:Dopamine / Q000737:chemistry*; D004563:Electrochemistry; D004867:Equipment Design; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D010084:Oxidation-Reduction; D012680:Sensitivity and Specificity
chemical_list: D004247:DNA; D048271:Chitosan; D004298:Dopamine