Estimation of population heterozygosity and library construction-induced mutation rate from expressed sequence tag collections.
Unigene alignments obtained from cDNA libraries made using multiple individuals are not currently used to estimate population heterozygosity, as they are known to harbor mutations created during library construction. We describe an estimator of population heterozygosity that utilizes only SNPs unlikely to be library construction artifacts.
Long
A D
AD
Ecology and Evolutionary Biology, University of California, Irvine, California 92697, USA. tdlong@uci.edu
Beldade
P
P
Macdonald
S J
SJ
D000483:Alleles; D000818:Animals; D002080:Butterflies / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics; D020224:Expressed Sequence Tags; D015723:Gene Library; D006579:Heterozygote; D009154:Mutation / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics