Endoplasmic reticulum retention signal-dependent glycylation of the Hsp70/Grp170-related Pgp1p in Tetrahymena.
Glycylation is an uncommon posttranslational modification. It has been found that tubulin glycylation is essential for cell survival in Tetrahymena. Here we describe PGP1, a Tetrahymena gene encoding an Hsp70 homologue that is a novel glycylated protein. Pgp1p is a conserved glycoprotein that localizes within the lumen of the endoplasmic reticulum (ER). We demonstrate that PGP1 is essential for viability and present evidence that both glycosylation and ER retention are necessary but not sufficient for glycylation.
Xie
Rong
R
Department of Biology, University of Rochester, 425 Hutchison Hall, Rochester, NY 14627, USA.
Clark
Kathleen M
KM
Gorovsky
Martin A
MA
D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D004721:Endoplasmic Reticulum / Q000235:genetics / Q000378:metabolism*; D005973:Glutamine / Q000378:metabolism; D005998:Glycine / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D021382:Protein Sorting Signals / Q000502:physiology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013768:Tetrahymena / Q000235:genetics* / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism
D018528:ATP-Binding Cassette Transporters; D006023:Glycoproteins; D018840:HSP70 Heat-Shock Proteins; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; C098410:glucose-regulated protein 170; D005973:Glutamine; D014443:Tyrosine; D005998:Glycine