[Effects of interleukin 13 on the differentiation and expression of transcription factor c-fos of HEL cells].
OBJECTIVE
To study the effects of IL-13 on the differentiation and expression of transcription factor c-fos of human erythroleukemia cell line (HEL) cells.


METHODS
Reverse transcription polymerase chain reaction (RT-PCR) was used to observe the mRNA expression of IL-13 receptor a 1, GP i b, vWF and c-fos, and Western blot and cytometry were used to analyse their protein expression.


RESULTS
IL-13 receptor a 1 was expressed on HEL cells. IL-13 (100 ng/ml ) up-regulated the mRNA expression of GP II b and vWF. The ratio of luminous absorption (LA) of GP I b to p-actin bands ( AB) was 1. 303 in control group, whereas was 2. 912 in experiment group; being 2. 23-fold higher than that in control group (P < 0. 05). The ratio of LA to AB for vWF was 0.217 in control group, and 0. 506 in experiment group; indicating a 2. 33-fold increase in experiment group (P <0. 05). The protein expression of GP I b and vWF was significantly increased in experiment group, compared with that in control group. IL-13 inducing the increased expression of c-fos mRNA and protein of HEL cells peaked at 30 min and 60 min, respectively. The ratio of LA to AB for c-fos was also increased at 30 min and 60 min (P <0. 05).


CONCLUSIONS
IL-13 prompts the differentiation of HEL cells and up-regulates the expression of c-fos.
Shi
Xiao-yu
XY
Department of Histology and Enbryology, Basic Medical College, Nanchang University, Nanchang 330006, China.
Yu
Xue-mei
XM
Li
Wen-lin
WL
Wang
Zhi-gang
ZG
Tang
Hong-lin
HL
Zhang
Xue-jun
XJ
D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D006801:Humans; D018793:Interleukin-13 / Q000494:pharmacology*; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism*; D040201:Platelet Membrane Glycoprotein IIb / Q000096:biosynthesis / Q000235:genetics; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D053660:Receptors, Interleukin-13 / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation; D014841:von Willebrand Factor / Q000096:biosynthesis / Q000235:genetics
D018793:Interleukin-13; D040201:Platelet Membrane Glycoprotein IIb; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D053660:Receptors, Interleukin-13; D014841:von Willebrand Factor