title: Pyocyanin isolated from a marine microbial population: synergistic production between two distinct bacterial species and mode of action.
abstract: Marine microbial populations collected from the Hawaiian Islands were screened for antimicrobial activity. A blue metabolite was identified from mixed cell cultures, but production was not evident in pure cultures. Experiments designed to probe the synergistic role of the microorganisms are presented. Full characterization of the blue natural product, pyocyanin, is provided including corrections made to 1H and 13C-NMR assignments of the molecule misreported in the chemical literature and yeast transcriptome analysis. The transcriptional effects were consistent with the compound's purported role as an inducer of oxidative stress and damage and illustrates the overall potential of the method to reveal the primary biological/cellular effects of a natural product. The experiments outlined here might serve as a general paradigm for identification of natural products arising from microbial communities and investigation of their respective interactions.
authors.affiliation: Department of Chemistry, Texas A&M University, College Station, Texas 77843, USA.
authors.forename: Scott
authors.initials: S
authors.lastname: Angell
authors.forename: Bennie J
authors.initials: BJ
authors.lastname: Bench
authors.forename: Howard
authors.initials: H
authors.lastname: Williams
authors.forename: Coran M H
authors.initials: CM
authors.lastname: Watanabe
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005656:Fungal Proteins / Q000187:drug effects* / Q000235:genetics; D020128:Inhibitory Concentration 50; D008386:Marine Biology; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D011710:Pyocyanine / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014158:Transcription, Genetic; D014871:Water Microbiology
chemical_list: D005656:Fungal Proteins; D011710:Pyocyanine