title: Use of rolling-circle amplification for large-scale yeast two-hybrid analyses.
abstract: Detection of protein-protein interactions on a large-scale has become a major focus of functional genomics after the completion of genome sequencing. The information generated from these studies not only assembles proteins into signaling networks, but also reveals potential functions of uncharacterized proteins when their interacting partners have known functions. We have developed a rolling circle amplification-based yeast two-hybrid scheme that allows one to test reproducibility and specificity of the interactions on a large scale. Using this scheme, technical false-positives from yeast two-hybrid analyses can be efficiently minimized.
authors.affiliation: Department of Plant Pathology, University of Florida, Gainesville, USA.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Ding
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wen-Yuan
authors.initials: WY
authors.lastname: Song
mesh_terms: D004261:DNA Replication; D015723:Gene Library; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010957:Plasmids / Q000096:biosynthesis* / Q000235:genetics; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D012680:Sensitivity and Specificity; D014170:Transformation, Genetic; D020798:Two-Hybrid System Techniques