title: SILENCE: a new forward genetic technology.
abstract: Silencing induced by long terminal repeat (LTR)-encoded cis-acting response element, termed SILENCE, is a forward genetic system that allows for conditional, epigenetic control of host-gene transcription. This new research tool is independent of gene mutation or disruption, does not require complementation, and conditional gene repression appears complete at the level of protein function. SILENCE functions in hypodiploid cells and is a platform technology with broad applications in gene discovery.
authors.affiliation: Department of Microbiology, Immunology and Molecular Genetics, 609 Charles E. Young Dr. East, Molecular Sciences Building, University of California at Los Angeles (UCLA), Los Angeles, California 90095, USA.
authors.forename: David J
authors.initials: DJ
authors.lastname: Banks
authors.forename: Kenneth A
authors.initials: KA
authors.lastname: Bradley
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000494:pharmacology; D001427:Bacterial Toxins / Q000494:pharmacology; D016466:CHO Cells; D002999:Clone Cells; D006224:Cricetinae; D003412:Cricetulus; D020868:Gene Silencing; D005821:Genetic Techniques; D009154:Mutation; D010641:Phenotype; D018000:Receptors, Peptide / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D013329:Structure-Activity Relationship; D020079:Terminal Repeat Sequences / Q000235:genetics
chemical_list: D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D018000:Receptors, Peptide; C030325:anthrax toxin; C070309:anthrax toxin receptors