title: Electrochemically generated acid and its containment to 100 micron reaction areas for the production of DNA microarrays.
abstract: An addressable electrode array was used for the production of acid at sufficient concentration to allow deprotection of the dimethoxytrityl (DMT) protecting group from an overlaying substrate bound to a porous reaction layer. Containment of the generated acid to an active electrode of 100 micron diameter was achieved by the presence of an organic base. This procedure was then used for the production of a DNA array, in which synthesis was directed by the electrochemical removal of the DMT group during synthesis. The product array was found to have a detection sensitivity to as low as 0.5 pM DNA in a complex background sample.
authors.affiliation: CombiMatrix Corporation, Mukilteo, Washington, United States of America. kmaurer@combimatrix.com
authors.forename: Karl
authors.initials: K
authors.lastname: Maurer
authors.forename: John
authors.initials: J
authors.lastname: Cooper
authors.forename: Marcelo
authors.initials: M
authors.lastname: Caraballo
authors.forename: James
authors.initials: J
authors.lastname: Crye
authors.forename: Dominic
authors.initials: D
authors.lastname: Suciu
authors.forename: Andrey
authors.initials: A
authors.lastname: Ghindilis
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Leonetti
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Francis M
authors.initials: FM
authors.lastname: Rossi
authors.forename: Axel G
authors.initials: AG
authors.lastname: Stöver
authors.forename: Christopher
authors.initials: C
authors.lastname: Larson
authors.forename: Hetian
authors.initials: H
authors.lastname: Gao
authors.forename: Kilian
authors.initials: K
authors.lastname: Dill
authors.forename: Andy
authors.initials: A
authors.lastname: McShea
mesh_terms: D000143:Acids; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D055664:Electrochemical Techniques / Q000379:methods*; D007202:Indicators and Reagents; D008832:Microchemistry; D008839:Microelectrodes; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*
chemical_list: D000143:Acids; D017931:DNA Primers; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides