title: Novel peptide-based copper(II) complexes for total hydrolytic cleavage of DNA.
abstract: Stable Cu(II) complexes with histamine- and histidine-containing dipeptides histidylserine and histidylphenylalanine have been developed. Their interaction in solution has been investigated, and the stability of their complexes was determined. The nature of binding in these complexes has been explained with the help of potentiometric pH titrations and 1H-NMR spectroscopy. The geometry of these complexes has been established by electronic spectra. The DNA-binding and -cleavage abilities of these Cu(II) complexes have been probed by the absorption, thermal denaturation, fluorescence, and electrophoresis experiments. The results suggest that these peptide-based Cu(II) complexes effectively bind and efficiently cleave DNA under mild biological conditions. Since Cu(II) complexes are known to play an important role in phosphodiester bond cleavages, these results assume importance.
authors.affiliation: Department of Chemistry, Osmania University, Hyderabad 500007, India. rabi_pr@rediffmail.com
authors.forename: Pulimamidi R
authors.initials: PR
authors.lastname: Reddy
authors.forename: Pallerla
authors.initials: P
authors.lastname: Manjula
authors.forename: Sepuru K
authors.initials: SK
authors.lastname: Mohan
mesh_terms: D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004151:Dipeptides / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D015394:Molecular Structure
chemical_list: D004151:Dipeptides; C516949:histidylserine; C022144:histidylphenylalanine; D003300:Copper; D004247:DNA