Investigating the effects of preinduction on human adipose-derived precursor cells in an athymic rat model.
The osteogenic potential of human adipose-derived precursor cells seeded on medical-grade polycaprolactone-tricalcium phosphate scaffolds was investigated in this in vivo study. Three study groups were investigated: (1) induced--stimulated with osteogenic factors only after seeding into scaffold; (2) preinduced--induced for 2 weeks before seeding into scaffolds; and (3) uninduced--cells without any introduced induction. For all groups, scaffolds were implanted subcutaneously into the dorsum of athymic rats. The scaffold/cell constructs were harvested at the end of 6 or 12 weeks and analyzed for osteogenesis. Gross morphological examination using scanning electron microscopy indicated good integration of host tissue with scaffold/cell constructs and extensive tissue infiltration into the scaffold interior. Alizarin Red histology and immunostaining showed a heightened level of mineralization and an increase in osteonectin, osteopontin, and collagen type I protein expression in both the induced and preinduced groups compared with the uninduced groups. However, no significant differences were observed in these indicators when compared between the induced and preinduced groups.
Leong
David Tai
DT
Department of Biological Sciences, National University of Singapore, Singapore 117576, Republic of Singapore.
Abraham
Mohan Chothirakottu
MC
Rath
Subha Narayan
SN
Lim
Thiam-Chye
TC
Chew
Fook Tim
FT
Hutmacher
Dietmar Werner
DW
D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D002130:Calcium Phosphates / Q000494:pharmacology; D002454:Cell Differentiation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000032:analysis / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008855:Microscopy, Electron, Scanning; D023421:Models, Animal; D010012:Osteogenesis; D015676:Osteonectin / Q000032:analysis / Q000378:metabolism; D053495:Osteopontin / Q000032:analysis / Q000378:metabolism; D011091:Polyesters / Q000494:pharmacology; D051381:Rats; D011923:Rats, Nude; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism
D001672:Biocompatible Materials; D002130:Calcium Phosphates; D024042:Collagen Type I; D015676:Osteonectin; D011091:Polyesters; D053495:Osteopontin; C016240:polycaprolactone; C018392:tricalcium phosphate