Use of rolling-circle amplification for large-scale yeast two-hybrid analyses.
Detection of protein-protein interactions on a large-scale has become a major focus of functional genomics after the completion of genome sequencing. The information generated from these studies not only assembles proteins into signaling networks, but also reveals potential functions of uncharacterized proteins when their interacting partners have known functions. We have developed a rolling circle amplification-based yeast two-hybrid scheme that allows one to test reproducibility and specificity of the interactions on a large scale. Using this scheme, technical false-positives from yeast two-hybrid analyses can be efficiently minimized.
Ding
Xiaodong
X
Department of Plant Pathology, University of Florida, Gainesville, USA.
Zhang
Yan
Y
Song
Wen-Yuan
WY
D004261:DNA Replication; D015723:Gene Library; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010957:Plasmids / Q000096:biosynthesis* / Q000235:genetics; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D012680:Sensitivity and Specificity; D014170:Transformation, Genetic; D020798:Two-Hybrid System Techniques