Effects of the magnesium and chloride ions and shikimate on the structure of shikimate kinase from Mycobacterium tuberculosis.
Bacteria, fungi and plants can convert carbohydrate and phosphoenolpyruvate into chorismate, which is the precursor of various aromatic compounds. The seven enzymes of the shikimate pathway are responsible for this conversion. Shikimate kinase (SK) is the fifth enzyme in this pathway and converts shikimate to shikimate-3-phosphate. In this work, the conformational changes that occur on binding of shikimate, magnesium and chloride ions to SK from Mycobacterium tuberculosis (MtSK) are described. It was observed that both ions and shikimate influence the conformation of residues of the active site of MtSK. Magnesium influences the conformation of the shikimate hydroxyl groups and the position of the side chains of some of the residues of the active site. Chloride seems to influence the affinity of ADP and its position in the active site and the opening length of the LID domain. Shikimate binding causes a closing of the LID domain and also seems to influence the crystallographic packing of SK. The results shown here could be useful for understanding the catalytic mechanism of SK and the role of ions in the activity of this protein.
Dias
Marcio Vinicius Bertacine
MV
Programa de Pós-Graduação em Biofísica Molecular, Departamento de Física, UNESP, São José do Rio Preto, SP 15054-000, Brazil.
Faím
Lívia Maria
LM
Vasconcelos
Igor Bordin
IB
de Oliveira
Jaim Simões
JS
Basso
Luiz Augusto
LA
Santos
Diógenes Santiago
DS
de Azevedo
Walter Filgueira
WF
D001665:Binding Sites; D002712:Chlorides / Q000737:chemistry / Q000378:metabolism*; D018360:Crystallography, X-Ray; D008274:Magnesium / Q000737:chemistry / Q000378:metabolism*; D009169:Mycobacterium tuberculosis / Q000201:enzymology*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000737:chemistry* / Q000378:metabolism; D017433:Protein Structure, Secondary; D012765:Shikimic Acid / Q000737:chemistry / Q000378:metabolism*
D002712:Chlorides; D012765:Shikimic Acid; D017853:Phosphotransferases (Alcohol Group Acceptor); C022642:shikimate kinase; D008274:Magnesium