title: [Study on trans-differentiation of adult human myoblasts into neural precursor cells and its implantation in rats].
abstract: OBJECTIVE
  To investigate the feasibility of inducing adult human myoblasts into neural precursor cells.
  
  
  METHODS
  The myoblasts were isolated with mixed digestive enzyme from minced human temporal muscle samples, cultured and purified clonally. The 3rd passage cells were incubated with serum free medium including basic fibroblast growth factor (bFGF), epidermal growth factor (EGF) and leukemia inhibitory factor (LIF). Morphological change was investigated during incubation period. Immunofluorescence cytochemistry and RT-PCR analysis were used to assess cell differentiation and trans differentiation.
  
  
  RESULTS
  After the induction, cells became non-adherent aggregates as neurospheres. The myoblast-derived neurospheres was immuno-positive for nestin. In differentiation condition, they looked like neurons and glial cells and expressed neuronal (microtubule associated protein 2, MAP-2), astrocytic (Glial fibrillary acidic protein, GFAP) and oligodendrocytic (Galactocerebroside, Galc) markers by immunocytochemistry. The result by RT-PCR was coincident with immunocytochemistry. The myoblast-derived neurospheres expressed MAP-2 and GFAP after they were transplanted into the brain of rats with cerebral ischemia.
  
  
  CONCLUSIONS
  Adult human myoblasts can be inducted to trans-differentiate into neural precursor cells.
authors.affiliation: Department of Neurosurgery, Peking Union Medical College Hospital, Peking Union College and Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Zhen-xing
authors.initials: ZX
authors.lastname: Zhang
authors.forename: Ren-zhi
authors.initials: RZ
authors.lastname: Wang
authors.forename: Gui-lin
authors.initials: GL
authors.lastname: Li
authors.forename: Wan-chen
authors.initials: WC
authors.lastname: Dou
authors.forename: Shi-fang
authors.initials: SF
authors.lastname: Li
authors.forename: Jun-ji
authors.initials: JJ
authors.lastname: Wei
authors.forename: Yu-kui
authors.initials: YK
authors.lastname: Wei
authors.forename: Fei-fan
authors.initials: FF
authors.lastname: Zhao
authors.forename: Yan-guo
authors.initials: YG
authors.lastname: Kong
authors.forename: Hai-tao
authors.initials: HT
authors.lastname: Wu
authors.forename: Ming
authors.initials: M
authors.lastname: Fan
mesh_terms: D000328:Adult; D000818:Animals; D002454:Cell Differentiation; D048430:Cell Shape / Q000187:drug effects; D054338:Cell Transdifferentiation; D017690:Cell Transplantation; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D053605:Leukemia Inhibitory Factor / Q000494:pharmacology; D008297:Male; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis / Q000235:genetics; D032446:Myoblasts / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D009416:Nerve Regeneration; D009474:Neurons / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014183:Transplantation, Heterologous
chemical_list: D005904:Glial Fibrillary Acidic Protein; D053605:Leukemia Inhibitory Factor; D008869:Microtubule-Associated Proteins; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor