title: Effect of roughness on particle adhesion in aqueous solutions: a study of Saccharomyces cerevisiae and a silica particle.
abstract: An atomic force microscope (AFM) has been used to quantify the adhesion of living cells Saccharomyces cerevisiae on three different silica surfaces with defined roughness. The effects of support roughness on the adhesion forces of a smooth silica particle were studied in addition. A living single cell was immobilized at the apex of a tipless AFM cantilever using a key-lock mechanism. Adhesion was quantified from the force-distance data measured on a smooth silica substrate and two substrates coated with hydrophilic monodisperse silica particles with 110 and 240 nm in diameter to study the effect of roughness on particle adhesion. The AFM technique gives unique insight into the primary colonization event of biofilm formation. The new knowledge helps substantially to design surface coatings relevant for biotechnology, medicine and dentistry.
authors.affiliation: 3M ESPE, ESPE Platz, 82229 Seefeld, Germany. martin.goetzinger@mmm.com
authors.forename: Martin
authors.initials: M
authors.lastname: Götzinger
authors.forename: Bernhard
authors.initials: B
authors.lastname: Weigl
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Peukert
authors.forename: Karl
authors.initials: K
authors.lastname: Sommer
mesh_terms: D000327:Adsorption; D002448:Cell Adhesion / Q000502:physiology; D004735:Energy Transfer; D006863:Hydrogen-Ion Concentration; D018625:Microscopy, Atomic Force / Q000379:methods; D010316:Particle Size; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*; D012996:Solutions / Q000737:chemistry; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: D012996:Solutions; D014867:Water; D012822:Silicon Dioxide