title: Redox proteomics identification of oxidatively modified brain proteins in inherited Alzheimer's disease: an initial assessment.
abstract: OBJECTIVE
  To identify oxidatively modified proteins in brains of persons with inherited Alzheimer's disease.
  
  
  METHODS
  Redox proteomics was used to identify oxidatively modified brain proteins in persons with mutations in the genes for presenilin-1 (PS-1).
  
  
  RESULTS
  An initial redox proteomics assessment of oxidatively modified proteins from brains of individuals with PS-1 mutations was performed. These PS1 mutations, Q222H and M233T, are completely penetrant causing early-onset familial AD as previously reported in these Australian families. We show that oxidative modifications of ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1), gamma-enolase, actin, and dimethylarginine dimethylaminohydrolase 1 (DMDMAH-1) are present in the brain of familial AD subjects.
  
  
  CONCLUSIONS
  These initial results suggest that oxidatively modified proteins are important common features in both familial and sporadic AD.
authors.affiliation: Department of Chemistry, Center of Membrane Sciences, and Sanders-Brown Center on Aging, University of Kentucky, Lexington, KY 40506-0055, USA. dabcns@uky.edu
authors.forename: D Allan
authors.initials: DA
authors.lastname: Butterfield
authors.forename: Anastazija
authors.initials: A
authors.lastname: Gnjec
authors.forename: H Fai
authors.initials: HF
authors.lastname: Poon
authors.forename: Alessandra
authors.initials: A
authors.lastname: Castegna
authors.forename: William M
authors.initials: WM
authors.lastname: Pierce
authors.forename: Jon B
authors.initials: JB
authors.lastname: Klein
authors.forename: Ralph N
authors.initials: RN
authors.lastname: Martins
mesh_terms: D000199:Actins / Q000235:genetics; D000544:Alzheimer Disease / Q000235:genetics* / Q000503:physiopathology; D000581:Amidohydrolases / Q000235:genetics; D001921:Brain / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000235:genetics* / Q000502:physiology; D010751:Phosphopyruvate Hydratase; D053764:Presenilin-1 / Q000235:genetics*; D040901:Proteomics; D043222:Ubiquitin Thiolesterase / Q000235:genetics
chemical_list: D000199:Actins; D053764:Presenilin-1; C482860:UCHL1 protein, human; D043222:Ubiquitin Thiolesterase; D000581:Amidohydrolases; C059999:dimethylargininase; D010751:Phosphopyruvate Hydratase