title: Synthesis of oligonucleotide-peptide conjugates carrying the c-myc peptide epitope as recognition system.
abstract: Oligonucleotide-peptide conjugates 1-3 were prepared by sequential addition of the appropriate Boc-protected amino acids, followed by nucleoside phosphoramidites in the same support. These molecules are designed to be used for triplex formation and for the directed assembly of nanomaterials. The structures of the desired oligonucleotide-peptide conjugates were confirmed by mass spectrometry on small oligonucleotide-peptide conjugates, by gel electrophoresis, and by hybridization with complementary oligonucleotides. Oligonucleotides carrying the c-myc peptide were specifically recognized by the anti-c-myc monoclonal antibody.
authors.affiliation: Department of Structural Biology, Instituto de Biología Molecular de Barcelona, C.S.I.C., Jordi Girona 18-26, E-08034 Barcelona.
authors.forename: Miriam
authors.initials: M
authors.lastname: Frieden
authors.forename: Anna
authors.initials: A
authors.lastname: Aviñó
authors.forename: Gema
authors.initials: G
authors.lastname: Tarrasón
authors.forename: Marta
authors.initials: M
authors.lastname: Escorihuela
authors.forename: Jaume
authors.initials: J
authors.lastname: Piulats
authors.forename: Ramon
authors.initials: R
authors.lastname: Eritja
mesh_terms: D001665:Binding Sites / Q000502:physiology; D000939:Epitopes / Q000737:chemistry / Q000378:metabolism*; D049329:Nanostructures / Q000737:chemistry; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000378:metabolism; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis* / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000138:chemical synthesis* / Q000378:metabolism
chemical_list: D000939:Epitopes; D009841:Oligonucleotides; D020135:Peptide Nucleic Acids; D016271:Proto-Oncogene Proteins c-myc