title: Specificities of acetoxy derivatives of coumarins, biscoumarins, chromones, flavones, isoflavones and xanthones for acetoxy drug: protein transacetylase.
abstract: The earlier work carried out in our laboratory led to the identification of a novel rat liver microsomal enzyme termed as acetoxy drug: protein transacetylase (TAase), catalyzing the transfer of acetyl group from polyphenolic acetates (PA) to functional proteins. In this paper, we have reported the comparison of the specificities of acetoxy derivatives of coumarins, biscoumarins, chromones, flavones, isoflavones and xanthones with special reference to the phenyl moiety/bulky group on the pyran ring of PA. The results clearly indicated that compounds having phenyl moieties, when used as the substrates, resulted in a significant reduction of TAase catalyzed activity. The alteration in TAase catalyzed activation of NADPH cytochrome c reductase and inhibition of benzene-induced micronuclei in bone marrow cells by PA were in tune with their specificities to TAase.
authors.affiliation: Department of Biochemistry, V P Chest Institute, University of Delhi, Delhi 110 007, India.
authors.forename: Ajit
authors.initials: A
authors.lastname: Kumar
authors.forename: Brajendra K
authors.initials: BK
authors.lastname: Singh
authors.forename: Nawal K
authors.initials: NK
authors.lastname: Sharma
authors.forename: Kapil
authors.initials: K
authors.lastname: Gyanda
authors.forename: Sapan K
authors.initials: SK
authors.lastname: Jain
authors.forename: Yogesh K
authors.initials: YK
authors.lastname: Tyagi
authors.forename: Anil S
authors.initials: AS
authors.lastname: Baghel
authors.forename: Mukesh
authors.initials: M
authors.lastname: Pandey
authors.forename: Sunil K
authors.initials: SK
authors.lastname: Sharma
authors.forename: Ashok K
authors.initials: AK
authors.lastname: Prasad
authors.forename: Subhash C
authors.initials: SC
authors.lastname: Jain
authors.forename: Ramesh C
authors.initials: RC
authors.lastname: Rastogi
authors.forename: Hanumantharao G
authors.initials: HG
authors.lastname: Raj
authors.forename: Arthur C
authors.initials: AC
authors.lastname: Watterson
authors.forename: Erik
authors.initials: E
authors.lastname: Van der Eycken
authors.forename: Virinder S
authors.initials: VS
authors.lastname: Parmar
mesh_terms: D000107:Acetylation; D000123:Acetyltransferases / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D002384:Catalysis; D002867:Chromones / Q000737:chemistry*; D003374:Coumarins / Q000737:chemistry*; D015195:Drug Design; D047309:Flavones / Q000737:chemistry*; D007529:Isoflavones / Q000737:chemistry*; D008099:Liver / Q000201:enzymology; D008297:Male; D015162:Micronucleus Tests; D008861:Microsomes / Q000201:enzymology; D015394:Molecular Structure; D011714:Pyrans / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D044004:Xanthones / Q000737:chemistry*
chemical_list: D002867:Chromones; D003374:Coumarins; D047309:Flavones; D007529:Isoflavones; D011714:Pyrans; D044004:Xanthones; D000123:Acetyltransferases