title: Comparison of biopsy-based methods for the detection of Helicobacter pylori infection.
abstract: Various biopsy-based methods for the detection of Helicobacter pylori are evaluated to determine their sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV), followed by polymerase chain reaction (PCR) for the 16S ribosomal RNA (rRNA) gene of H. pylori (16S PCR) to confirm the results. Seventyfive patients (65% [49] males, age range: 17-77 years, mean 42+/-14.6 years) with dyspeptic symptoms are included in the study. Gastric antrum biopsy specimens collected during endoscopy are tested using a urea agar base enriched with 40% urea solution (eUAB, Oxoid)), a commercial rapid urease test (Pronto Dry, Medical Instrument Corp, Switzerland), histopathology and 16S PCR. The eUAB test showed 97% sensitivity, 86% specificity, 84% PPV, 97% NPV and 91% accuracy when the diagnosis of H. pylori infection was made with positive Pronto Dry and histopathology. Pronto Dry showed 100% sensitivity, 82% specificity, 80% PPV, 100% NPV and 89% accuracy when the diagnosis of H. pylori infection was made on positive histopathology and eUAB. Thus, the eUAB can be used as a rapid urease test. It is economical and has a sensitivity and specificity comparable to a commercially available rapid urease test to detect urease activity of H. pylori in gastric biopsy.
authors.affiliation: Department of Medicine and Pathology, Aga Khan University Hospital, Karachi, Pakistan. yakoobjaved@hotmail.com
authors.forename: J
authors.initials: J
authors.lastname: Yakoob
authors.forename: S
authors.initials: S
authors.lastname: Abid
authors.forename: W
authors.initials: W
authors.lastname: Jafri
authors.forename: Z
authors.initials: Z
authors.lastname: Abbas
authors.forename: M
authors.initials: M
authors.lastname: Islam
authors.forename: Z
authors.initials: Z
authors.lastname: Ahmad
mesh_terms: D003365:Costs and Cost Analysis; D003906:Developing Countries; D005753:Gastric Mucosa / Q000201:enzymology* / Q000382:microbiology / Q000473:pathology; D005773:Gastroscopy / Q000191:economics; D016481:Helicobacter Infections / Q000175:diagnosis*; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans; D010154:Pakistan; D011933:Reagent Kits, Diagnostic; D021521:Ribotyping / Q000191:economics; D012680:Sensitivity and Specificity; D014510:Urease / Q000032:analysis
chemical_list: D011933:Reagent Kits, Diagnostic; D014510:Urease