title: Influence of the period after ovariectomy on femoral and mandibular bone density and on induced periodontal disease.
abstract: BACKGROUND
  This study investigated the influence of the period after ovariectomy on femoral and mandibular bone mineral density (BMD) and on induced periodontal disease.
  
  
  METHODS
  One hundred and twenty-six female Holtzman rats were divided into nine groups: control, sham surgery (SHAM) with and without induction of periodontal disease for 51 and 150 days, and ovariectomy (OVX) with and without induction of periodontal disease for 51 and 150 days. Periodontal disease was induced by placing ligatures on the first lower molars during the last 30 days of each period. BMD was measured by dual-energy x-ray absorptiometry. Vertical bone loss was determined by measuring the distance from the alveolar bone crest to the cemento-enamel junction on the mesial side of the first lower molar.
  
  
  RESULTS
  Statistical analyses (Kruskal-Wallis test) revealed a significant difference between the OVX and SHAM groups' global and femoral proximal epiphysis BMD (P <0.001) for 150 days and in the global evaluation for 51 days. For mandibular BMD, no difference was found between the groups of each period. Influence of the period on femoral BMD was found only for the SHAM groups, with lower BMD for the 51-day period compared to the 150-day period (P <0.05). In the global evaluation of the mandible, a lower BMD was found after 51 days. The period was a contributing factor for the vertical bone loss, and it resulted in higher values for the 51-day period (P <0.05).
  
  
  CONCLUSIONS
  The period influenced the femoral BMD and the vertical bone loss in induced periodontal disease.
authors.affiliation: Department of Oral Diagnosis and Surgery, School of Dentistry of Araraquara, State University of São Paulo, Araraquara, SP, Brazil. s-orrico@foar.unesp.br
authors.forename: Silvana R P
authors.initials: SR
authors.lastname: Orrico
authors.forename: Gabriela
authors.initials: G
authors.lastname: Giro
authors.forename: Daniela
authors.initials: D
authors.lastname: Gonçalves
authors.forename: Lilian
authors.initials: L
authors.lastname: Takayama
authors.forename: Rosa M R
authors.initials: RM
authors.lastname: Pereira
mesh_terms: D016301:Alveolar Bone Loss / Q000209:etiology* / Q000503:physiopathology; D000818:Animals; D015519:Bone Density; D005260:Female; D005269:Femur / Q000502:physiology; D008334:Mandible / Q000502:physiology; D010024:Osteoporosis / Q000150:complications*; D010052:Ovariectomy; D010053:Ovary / Q000502:physiology*; D011897:Random Allocation; D051381:Rats; D018709:Statistics, Nonparametric