title: The ORF YNL274c (GOR1) codes for glyoxylate reductase in Saccharomyces cerevisiae.
abstract: The enzyme glyoxylate reductase reversibly reduces glyoxylate to glycolate, or alternatively hydroxypyruvate to D-glycerate, using either NADPH or NADH as a co-factor. The enzyme has multiple metabolic roles in different organisms. In this paper we show that GOR1 (ORF YNL274c) encodes a glyoxylate reductase and not a hydroxyisocaproate dehydrogenase in Saccharomyces cerevisiae, even though it also has minor activity on alpha-ketoisocaproate. In addition, we show that deletion of the glyoxylate reductase-encoding gene leads to higher biomass concentration after diauxic shift.
authors.affiliation: VTT Technical Research Centre of Finland, P.O. Box 1000, FIN-02044 VTT, Finland. Eija.Rintala@vtt.fi
authors.forename: Eija
authors.initials: E
authors.lastname: Rintala
authors.forename: Juha-Pekka
authors.initials: JP
authors.lastname: Pitkänen
authors.forename: Maija-Leena
authors.initials: ML
authors.lastname: Vehkomäki
authors.forename: Merja
authors.initials: M
authors.lastname: Penttilä
authors.forename: Laura
authors.initials: L
authors.lastname: Ruohonen
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D006038:Glyoxylates / Q000378:metabolism; D008954:Models, Biological; D016254:Mutagenesis, Insertional; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism
chemical_list: D004271:DNA, Fungal; D006038:Glyoxylates; D000429:Alcohol Oxidoreductases; C020830:glyoxylate reductase; C031150:glyoxylic acid