Preparation of multifunctional glyconanoparticles as a platform for potential carbohydrate-based anticancer vaccines.
A novel platform for anticancer vaccines has been prepared using glyconanotechnology recently developed in our laboratory. Ten different multifunctional gold glyconanoparticles incorporating sialylTn and Lewis(y) antigens, T-cell helper peptides (TT) and glucose in well defined average proportions and with differing density have been synthesised in one step and characterised using NMR and TEM. Size and nature of the linker were crucial to control kinetics of S-Au bond formation and to achieve the desired ligand ratio on the gold clusters. The technology presented here opens the way for tailoring polyvalent anticancer vaccines candidates and drug delivery carriers with defined average chemical composition.
Ojeda
Rafael
R
Grupo de Carbohidratos, Laboratory of Glyconanotechnology, Instituto de Investigaciones Químicas-CSIC, Américo Vespucio, 49, E-41092 Seville, Spain.
de Paz
Jose Luis
JL
Barrientos
Africa G
AG
Martín-Lomas
Manuel
M
Penadés
Soledad
S
D000595:Amino Acid Sequence; D000818:Animals; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000737:chemistry; D019496:Cancer Vaccines / Q000138:chemical synthesis*; D002240:Carbohydrate Sequence; D005260:Female; D006001:Glycoconjugates / Q000737:chemistry*; D007983:Lewis Blood Group Antigens / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D036103:Nanotechnology; D019906:Nuclear Magnetic Resonance, Biomolecular; D013745:Tetanus Toxoid / Q000737:chemistry
D015295:Antigens, Tumor-Associated, Carbohydrate; D019496:Cancer Vaccines; D006001:Glycoconjugates; D007983:Lewis Blood Group Antigens; C094162:Lewis Y antigen; D013745:Tetanus Toxoid; C055491:sialosyl-Tn antigen