Synthesis and characterization of poly (amino ester) for slow biodegradable gene delivery vector.
Many therapeutic carrier materials were exploited for human gene therapy from viral to polymeric vectors. This research describes the evaluation of two biodegradable ester-bonded polymers synthesized by double-monomer polycondensation for a non-viral cationic polymer-based gene delivery system. The backbone was constructed to include inner tertiary amines and outer primary amines. Self-assembly with DNA resulted in the production of regularly nano-sized spherical polyplexes with good transfection efficiency, especially in the presence of serum. The polymers showed a relatively slow degradability for an amine-containing ester polymer, as they maintained DNA/polymer complex for 7 days in physiological buffer conditions. Finally, the low toxicity and slow degradation concluded these polymers reliable for long-term therapeutic applications.
Kim
Hyun Jin
HJ
School of Chemistry and Molecular Engineering, Seoul National University, San 56-1, Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
Kwon
Min Sung
MS
Choi
Joon Sig
JS
Kim
Bo Hye
BH
Yoon
Jae Keun
JK
Kim
Kwan
K
Park
Jong-Sang
JS
D002021:Buffers; D004247:DNA / Q000378:metabolism; D004355:Drug Stability; D018014:Gene Transfer Techniques; D006801:Humans; D053758:Nanoparticles; D011091:Polyesters / Q000138:chemical synthesis* / Q000627:therapeutic use* / Q000633:toxicity; D044967:Serum; D014162:Transfection
D002021:Buffers; D011091:Polyesters; D004247:DNA