title: Activity of sodium metabisulfite against planktonic and biofilm Staphylococcus species.
abstract: Biofilm-forming staphylococci cause a majority of intravascular catheter-related infections. We evaluated the effect of sodium metabisulfite, a preservative commonly added to intravenously administered pharmaceuticals as an antioxidant and previously used as a catheter lock solution, on planktonic and biofilm staphylococci at clinically encountered concentrations. Sodium metabisulfite exhibited bactericidal activity against planktonic Staphylococcus aureus, Staphylococcus lugdunensis, and Staphylococcus epidermidis at concentrations of 512, 512, and 1024 microg/mL, respectively. A concentration of 720 microg/mL inhibited cell growth by all 3 species in a biofilm formation assay. However, established S. aureus and S. lugdunensis biofilms showed less than 1.5 log10 decreases in viable cell counts when treated with 720 microg/mL of sodium metabisulfite for 24 h. These in vitro results suggest that the use of sodium metabisulfite as a catheter lock may inhibit staphylococcal colonization of catheters, thereby preventing catheter-related infection.
authors.affiliation: Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA.
authors.forename: Kristi L
authors.initials: KL
authors.lastname: Frank
authors.forename: Robin
authors.initials: R
authors.lastname: Patel
mesh_terms: D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D002408:Catheters, Indwelling / Q000382:microbiology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008826:Microbial Sensitivity Tests; D010592:Pharmaceutic Aids / Q000494:pharmacology*; D012826:Silicone Elastomers; D013210:Staphylococcus / Q000145:classification / Q000187:drug effects* / Q000254:growth & development* / Q000502:physiology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D013447:Sulfites / Q000494:pharmacology*
chemical_list: D010592:Pharmaceutic Aids; D012826:Silicone Elastomers; D013447:Sulfites; C005200:sodium metabisulfite