title: Muscarinic acetylcholine receptor activation increases transcellular transport of macromolecules across mouse and human intestinal epithelium in vitro.
abstract: The intestinal epithelium acts as a barrier restricting uptake of luminal macromolecules such as dietary antigens and microbes. Here, we examined the role of cholinergic signalling in the regulation of permeability to macromolecules. Mouse jejunum was mounted in Ussing chambers and permeability was determined by measuring the flux of the antigen-sized protein, horseradish peroxidase (HRP), across the tissue. Baseline HRP permeability was significantly reduced by neural blockade with tetrodotoxin or cholinergic muscarinic antagonism with atropine, suggesting that ongoing release of endogenous acetylcholine from enteric nerves regulates barrier function. Exogenous addition of the muscarinic agonist bethanechol caused significant increases in both HRP flux and the area of HRP-containing endosomes in enterocytes. Bethanechol-enhanced HRP flux was abrogated by the M3 receptor antagonist, 4-diphenylacetoxy-N-methylpiperidine methiodide (4-DAMP), the phospholipase A(2) inhibitor quinacrine, and the cyclooxygenase inhibitor indomethacin. Complementary in vitro studies showed direct effects of bethanechol on T84 epithelial cells, where increased HRP uptake was associated with increased F-actin, and increased cytosolic phospholipase A(2) (cPLA(2)) phosphorylation. Taken together, these results provide evidence for cholinergic regulation of transepithelial transport of macromolecules, mainly mediated by activation of M3 receptors with subsequent involvement of phospholipase A(2) and cyclooxygenase products.
authors.affiliation: Intestinal Disease Research Program, McMaster University, Hamilton, Ontario, Canada.
authors.forename: H L
authors.initials: HL
authors.lastname: Cameron
authors.forename: M H
authors.initials: MH
authors.lastname: Perdue
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001693:Biological Transport, Active; D015153:Blotting, Western; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000187:drug effects / Q000648:ultrastructure; D003600:Cytosol / Q000187:drug effects / Q000201:enzymology; D015194:Diffusion Chambers, Culture; D011992:Endosomes / Q000187:drug effects / Q000648:ultrastructure; D020895:Enterocytes / Q000187:drug effects / Q000648:ultrastructure; D004846:Epithelial Attachment / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D006735:Horseradish Peroxidase; D006801:Humans; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018721:Muscarinic Agonists / Q000494:pharmacology*; D010539:Permeability; D010741:Phospholipases A / Q000378:metabolism; D010766:Phosphorylation; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000199:Actins; D007202:Indicators and Reagents; D018721:Muscarinic Agonists; D011976:Receptors, Muscarinic; D006735:Horseradish Peroxidase; D011451:Prostaglandin-Endoperoxide Synthases; D010741:Phospholipases A