title: Gateway-based destination vectors for functional analyses of bacterial ORFeomes: application to the Min system in Brucella abortus.
abstract: Twenty Gateway-compatible destination vectors were constructed. The vectors comprise fluorescent and epitope fusion tags, various drug markers, and replication origins that should make them useful for exploring existing microbial ORFeomes. In an attempt to validate several of these vectors, we observed polar and oscillating localization of MinD in Brucella abortus.
authors.affiliation: Unité de Recherche en Biologie Moléculaire, University of Namur, 61 rue de Bruxelles, B-5000 Namur, Belgium.
authors.forename: Régis
authors.initials: R
authors.lastname: Hallez
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Letesson
authors.forename: Jean
authors.initials: J
authors.lastname: Vandenhaute
authors.forename: Xavier
authors.initials: X
authors.lastname: De Bolle
mesh_terms: D002003:Brucella abortus / Q000235:genetics* / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D020543:Proteome / Q000378:metabolism*; D014169:Transformation, Bacterial
chemical_list: D020543:Proteome