title: Chlorhexidine preserves dentin bond in vitro.
abstract: Loss of hybrid layer integrity compromises resin-dentin bond stability. Matrix metalloproteinases (MMPs) may be partially responsible for hybrid layer degradation. Since chlorhexidine inhibits MMPs, we hypothesized that chlorhexidine would decelerate the loss of resin-dentin bonds. Class I preparations in extracted third molars were sectioned into two halves. One half was customarily restored (etch-and-rinse adhesive/resin composite), and the other was treated with 2% chlorhexidine after being acid-etched before restoration. Specimens were stored in artificial saliva with/without protease inhibitors. Microtensile bond strengths and failure mode distribution under SEM were analyzed immediately after specimens' preparation and 6 months later. With chlorhexidine, significantly better preservation of bond strength was observed after 6 months; protease inhibitors in the storage medium had no effect. Failure analysis showed significantly less failure in the hybrid layer with chlorhexidine, compared with controls after 6 months. In conclusion, this in vitro study suggests that chlorhexidine might be useful for the preservation of dentin bond strength.
authors.affiliation: Department of Restorative Dentistry, Dental Materials, Piracicaba School of Dentistry, University of Campinas, Piracicaba, SP, Brazil.
authors.forename: M R O
authors.initials: MR
authors.lastname: Carrilho
authors.forename: R M
authors.initials: RM
authors.lastname: Carvalho
authors.forename: M F
authors.initials: MF
authors.lastname: de Goes
authors.forename: V
authors.initials: V
authors.lastname: di Hipólito
authors.forename: S
authors.initials: S
authors.lastname: Geraldeli
authors.forename: F R
authors.initials: FR
authors.lastname: Tay
authors.forename: D H
authors.initials: DH
authors.lastname: Pashley
authors.forename: L
authors.initials: L
authors.lastname: Tjäderhane
mesh_terms: D000134:Acid Etching, Dental; D000891:Anti-Infective Agents, Local / Q000737:chemistry*; D002710:Chlorhexidine / Q000737:chemistry*; D003188:Composite Resins / Q000737:chemistry; D001840:Dental Bonding; D003737:Dental Cavity Preparation / Q000145:classification; D003793:Dental Restoration, Permanent / Q000379:methods; D003804:Dentin / Q000648:ultrastructure*; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D011480:Protease Inhibitors / Q000737:chemistry; D012464:Saliva, Artificial / Q000737:chemistry; D013314:Stress, Mechanical; D013499:Surface Properties; D013718:Tensile Strength; D013997:Time Factors
chemical_list: D000891:Anti-Infective Agents, Local; D003188:Composite Resins; D017220:Dentin-Bonding Agents; D011480:Protease Inhibitors; D012464:Saliva, Artificial; D002710:Chlorhexidine