Cytokine gene expression in Walker 256: a comparison of variants A (aggressive) and AR (regressive).
Two variants of this Walker 256 tumor have been previously reported as Walker 256 A and variant AR. The variant A has more aggressive property than variant AR and can induce systemic effects such as anorexia, sodium and water retention, followed by weight loss and death. The mechanisms involved in enhancing tumor regression and progression in this model are still incompletely understood. In the present study, serum and spleen mononuclear cells and tumor cells from animals inoculated with variants A and AR, were isolated to investigate the TGF-beta, IL-12, IFN-gamma and TNF-alpha and relationship with anemia, weight of animals, weight of spleen, volume of tumor and osmotic fragility compared with controls inoculated with Ringer Lactate. Results demonstrate that the group inoculated with variant A, compared to variant AR, shows high levels of TGF-beta gene expression in both tumor tissue and spleen cells, no expression of IFN-gamma and a progressive and higher levels of IL-12 in tumor tissue without inflammatory infiltrate visualized by optical microscopy. These results suggest that the aggressively of variant A is relate to cytokine modulation, facilitating the growth and escape of tumor cells. Furthermore, IL-12 seems to be constitutively expressed in both tumor lineage A and AR.
De Almeida Salles Perroud
Ana Paula
AP
Laboratório de Imunologia & Alergia Experimental, Departamento de Clínica Médica, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, UNICAMP, PO Box 6111, Campinas/SP 13083-887, Brazil.
Ashimine
Rika
R
De Castro
Glaucia Monteiro
GM
Guimarães
Fernando
F
Vieira
Karla Priscila
KP
Aparecida Vilella
Conceição
C
Samico Cavalcanti
Tereza Cristina
TC
De Lima Zollner
Ricardo
R
D000740:Anemia / Q000097:blood / Q000235:genetics / Q000473:pathology; D000818:Animals; D001835:Body Weight; D002279:Carcinoma 256, Walker / Q000097:blood / Q000235:genetics* / Q000473:pathology; D016207:Cytokines / Q000097:blood / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D006454:Hemoglobins / Q000032:analysis; D007371:Interferon-gamma / Q000097:blood / Q000235:genetics; D018664:Interleukin-12 / Q000235:genetics; D008297:Male; D009929:Organ Size; D009996:Osmotic Fragility; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000378:metabolism / Q000473:pathology; D016212:Transforming Growth Factor beta / Q000097:blood / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D016207:Cytokines; D006454:Hemoglobins; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12; D007371:Interferon-gamma