ESI mass spectrometry and X-ray diffraction studies of adducts between anticancer platinum drugs and hen egg white lysozyme.
The interactions of cisplatin and its analogues, transplatin, carboplatin and oxaliplatin, with hen egg white lysozyme were analysed through ESI mass spectrometry, and the resulting metallodrug-protein adducts identified; the X-ray crystal structure of the cisplatin lysozyme derivative, solved at 1.9 A resolution, reveals selective platination of imidazole Nepsilon of His15.
Casini
Angela
A
Department of Chemistry, University of Florence, Via della Lastruccia 3, 50019, Sesto Fiorentino, Italy.
Mastrobuoni
Guido
G
Temperini
Claudia
C
Gabbiani
Chiara
C
Francese
Simona
S
Moneti
Gloriano
G
Supuran
Claudiu T
CT
Scozzafava
Andrea
A
Messori
Luigi
L
D000818:Animals; D001665:Binding Sites; D016190:Carboplatin / Q000737:chemistry / Q000378:metabolism; D002645:Chickens; D002945:Cisplatin / Q000031:analogs & derivatives / Q000737:chemistry* / Q000378:metabolism; D018360:Crystallography, X-Ray; D004529:Egg White; D008958:Models, Molecular; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism; D009944:Organoplatinum Compounds / Q000737:chemistry / Q000378:metabolism; D000077150:Oxaliplatin; D017671:Platinum Compounds / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D021241:Spectrometry, Mass, Electrospray Ionization
D009944:Organoplatinum Compounds; D017671:Platinum Compounds; D000077150:Oxaliplatin; C034697:transplatin; D016190:Carboplatin; C421864:hen egg lysozyme; D009113:Muramidase; D002945:Cisplatin