title: 16S rRNA gene-based analysis of microbial community by whole-genome amplification and minigel-single-strand conformation polymorphism technique.
abstract: We have developed an analytical technique for the 16S rRNA gene that comprises whole-genome amplification and the polymerase chain reaction (PCR)-minigel-single-strand conformation polymorphism technique (WGA-SSCP). Under optimal conditions, SSCP bands could be detected when genomic DNA from bacteria of interest comprised 0.5% or more of the specimen. This method will be effective for the identification of nonculturable bacteria in a microbial community.
authors.affiliation: Department of Biotechnology, Tokyo Technical College, Higashi, Kunitachi-shi, Tokyo 186-0002, Japan. ooto@tera-house.ac.jp
authors.forename: Michiei
authors.initials: M
authors.lastname: Oto
authors.forename: Wataru
authors.initials: W
authors.lastname: Suda
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Shinoyama
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis*; D004783:Environmental Microbiology; D016680:Genome, Bacterial; D016133:Polymerase Chain Reaction / Q000379:methods*; D018807:Polymorphism, Single-Stranded Conformational; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012680:Sensitivity and Specificity
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S