Colon submucosal microdialysis: a novel in vivo approach in barrier function assessment - a pilot study in rats.
During shock, prognosis of a patient depends largely on intestinal barrier function. The potency of gut epithelium to represent an obstacle to toxins is determined by the blood supply. All established methods of mucosal function determination necessitate the functional involvement of bloodstream. Microdialysis allows monitoring of extracellular substances in the gut submucosa, but its potential use for gut barrier integrity assessment is unknown. Twelve rats underwent perfusion of the descending colon either with 20 % ethanol or control medium (vehicle). Both media contained equal amounts of a radioactive tracer substance ((51)Cr-EDTA). Mucosal permeability for (51)Cr-EDTA was assessed by microdialysate to luminal perfusate activity ratios. Sampling was performed using the colon submucosal microdialysis technique. The group subjected to ethanol treatment had profound macro- and microscopical alterations in perfused colonic segment associated with a significant increase in tracer permeability during ethanol exposure (2.354+/-0.298 % for ethanol as opposed to 0.209+/-0.102 % for control group, p 0.01), which remained elevated for 60 min after cessation of ethanol administration (3.352+/-0.188 % for ethanol compared to 0.140+/-0.0838 % for the control group, p 0.001). Submucosal microdialysis with radioactive tracer substance can be considered a feasible and advantageous alternative of gut barrier function estimation. Parallel monitoring of local tissue chemistry with this method remains a challenge in the future.
Cibicek
N
N
Institute of Clinical Biochemistry and Diagnostics (UKBD), University Hospital (FN), Hradec Králové, Czech Republic. cibicek@seznam.cz
Živná
H
H
Zadák
Z
Z
Kulír
J
J
Čermáková
E
E
Palicka
V
V
D000818:Animals; D002860:Chromium Radioisotopes; D044683:Colon, Descending / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D004492:Edetic Acid / Q000031:analogs & derivatives / Q000493:pharmacokinetics; D000431:Ethanol / Q000633:toxicity; D005240:Feasibility Studies; D007408:Intestinal Absorption / Q000187:drug effects; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008297:Male; D017551:Microdialysis; D010477:Perfusion; D010539:Permeability; D010865:Pilot Projects; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar
D002860:Chromium Radioisotopes; D019275:Radiopharmaceuticals; D000431:Ethanol; D004492:Edetic Acid