UBPy/MSJ-1 system during male germ cell progression in the frog, Rana esculenta.
mUBPy (mouse ubiquitin specific processing protease) is a de-ubiquitinating enzyme expressed in mouse testis and brain. In testis, it interacts with the DnaJ protein MSJ-1 (mouse sperm cell specific DnaJ first homologue), a molecular chaperone expressed in spermatids and spermatozoa. Since MSJ-1 is conserved among vertebrates, to demonstrate an evolutionarily conserved function of UBPy/MSJ-1 system, we assayed mUBPy presence in the anuran amphibian, the frog, Rana esculenta, during the annual sexual cycle. By Western blot we have detected a specific signal of 126kDa in testis and isolated spermatozoa. During the annual sexual cycle, the signal gradually increases as soon as spermatogenesis resumes after the winter stasis. Using immunocytochemistry, we have localized the protein in spermatids and spermatozoa. In conclusion, UBPy/MSJ-1 system is available in R. esculenta testis suggesting a conserved fundamental function in spermatogenesis and sperm formation.
Meccariello
Rosaria
R
Dipartimento di Studi delle Istituzioni e dei Sistemi Territoriali, Università di Napoli Parthenope, Naples, Italy.
Chianese
Rosanna
R
Scarpa
Donatella
D
Berruti
Giovanna
G
Cobellis
Gilda
G
Pierantoni
Riccardo
R
Fasano
Silvia
S
D000818:Animals; D015153:Blotting, Western; D010450:Endopeptidases / Q000502:physiology*; D056827:Endosomal Sorting Complexes Required for Transport; D005854:Germ Cells / Q000378:metabolism* / Q000502:physiology; D050956:HSP40 Heat-Shock Proteins / Q000378:metabolism*; D008297:Male; D011893:Rana esculenta / Q000378:metabolism* / Q000502:physiology; D013091:Spermatogenesis / Q000502:physiology*; D043222:Ubiquitin Thiolesterase
D056827:Endosomal Sorting Complexes Required for Transport; D050956:HSP40 Heat-Shock Proteins; D010450:Endopeptidases; D043222:Ubiquitin Thiolesterase; C541536:Usp8 protein, mouse; C091939:ubiquitin isopeptidase