GABPalpha regulates Oct-3/4 expression in mouse embryonic stem cells.
Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of blastocysts, and transcription factors Oct-3/4, Nanog, Sox2, and STAT3, are essential for their self-renewal. In this study, we searched for molecules downstream of STAT3 in ES cells. Using DNA chip analysis, we obtained GA-repeat binding protein (GABP) alpha. Expression of GABPalpha was restricted to undifferentiated ES cells and controlled by STAT3. We found that the expression level of Oct-3/4 is reduced by knockdown of GABPalpha. On the other hand, GABPalpha-overexpressing ES cells maintained the expression level of Oct-3/4 even in the absence of LIF. Moreover, the induction of Oct-3/4 repressors Cdx-2, Coup-tf1, and GCNF was stimulated by GABPalpha knockdown. These data suggest that GABPalpha upregulates the expression of Oct-3/4 via downregulation of Oct-3/4 repressors.
Kinoshita
Keita
K
Department of Stem Cell Biology, Graduate School of Medical Science, Kanazawa University, 13-1 Takara-machi, Kanazawa, Ishikawa 920-8640, Japan.
Ura
Hiroki
H
Akagi
Tadayuki
T
Usuda
Masayuki
M
Koide
Hiroshi
H
Yokota
Takashi
T
D000818:Animals; D015536:Down-Regulation / Q000187:drug effects; D053595:Embryonic Stem Cells / Q000502:physiology*; D051268:GA-Binding Protein Transcription Factor / Q000502:physiology*; D005786:Gene Expression Regulation; D053605:Leukemia Inhibitory Factor / Q000502:physiology; D051379:Mice; D050814:Octamer Transcription Factor-3 / Q000096:biosynthesis* / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D034622:RNA Interference; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000502:physiology; D015854:Up-Regulation
D051268:GA-Binding Protein Transcription Factor; C495656:Gabpa protein, mouse; D053605:Leukemia Inhibitory Factor; D050814:Octamer Transcription Factor-3; D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse