Inhibition of mouse acrosome reaction and sperm-zona pellucida binding by anti-human sperm membrane protein 1 antibody.
OBJECTIVE
To investigate the possible functions of human sperm membrane protein (hSMP-1) in the process of fertilization.


METHODS
A 576-bp cDNA fragment of HSD-1 gene coding for the extracellular domain of hSMP-1 was cloned and expressed. The localization of this protein on human and mouse sperm was determined by indirect immunofluorescent staining by using anti-recombinant hSMP-1 (anti-rhSMP-1) antibodies. Sperm acrosome reaction and sperm-zona pellucida (ZP) binding assay were carried out in 10-week-old BALB/c mice.


RESULTS
Recombinant hSMP-1 was successfully cloned and expressed. The expression of the native protein was limited on the acrosome of human and mouse sperm. Treatment of anti-rhSMP-1 antibodies significantly decreased the average number of sperms bound to each egg. Meanwhile, the percentage of acrosome reaction was decreased in comparison to pre-immune control after treatment with anti-rhSMP-1 (P < 0.05).


CONCLUSIONS
The results suggest that anti-rhSMP-1 antibody inhibited mouse acrosome reaction and sperm-ZP binding.
Cheng
Guo-Yan
GY
Department of Histology and Embryology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
Shi
Jian-Li
JL
Wang
Min
M
Hu
Yan-Qin
YQ
Liu
Chun-Meng
CM
Wang
Yi-Fei
YF
Xu
Chen
C
D020101:Acrosome Reaction / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D000954:Antigens, Surface / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D005306:Fertilization; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015044:Zona Pellucida / Q000276:immunology / Q000502:physiology
D000906:Antibodies; D000954:Antigens, Surface; D017931:DNA Primers; D008565:Membrane Proteins; D011994:Recombinant Proteins; C099014:SPAG8 protein, human