title: Xenotransplantation of human ovarian tissue pieces in gonadotropin-stimulated SCID mice: the effect of ovariectomy.
abstract: The number of follicles were compared in different developmental stages after the cryopreservation of human ovarian tissue by open freezing system followed by xenotransplantation into severe combined immunodeficient (SCID)-mice under stimulation, with and without ovariectomy. Ovarian tissue, cryopreserved for fertility preservation was partly examined by LIVE/DEAD viability staining or was transplanted in the neck muscle of 32 SCID-mice. The development of follicles, estradiol production, vaginal cytology and uterus weight was assessed after 15 weeks under gonadotropin stimulation, with or without ovariectomy. Viable follicles were detected in all frozen/thawed specimens using the LIVE/DEAD assay. Ovariectomy caused a significant improvement of survival of follicles in the preantral and antral stages in the gonadotropin-stimulated animals (p < 0.001), whereas there was no significant effect on the primordial and primary follicle counts. In the non-ovariectomised group, only isolated primordial and primary follicles could be detected. The total follicle amount was significantly higher in the ovariectomised group (n=17, 9.2 +/- 7.8, mean +/-SD) than in the non-ovariectomized group (n=15, 0.3 +/- 1.0). This study demonstrates that ovariectomy of stimulated recipient SCID-mice is essential for the development of follicles after xenotransplantation of cryopreserved human ovarian grafts.
authors.affiliation: Department of Obstetrics and Gynecology, Johannes Gutenberg University, D-55116 Mainz, Germany. maltaris@uni-mainz.de
authors.forename: Theodoros
authors.initials: T
authors.lastname: Maltaris
authors.forename: Heinz
authors.initials: H
authors.lastname: Koelbl
authors.forename: Franz
authors.initials: F
authors.lastname: Fischl
authors.forename: Rudolf
authors.initials: R
authors.lastname: Seufert
authors.forename: Markus
authors.initials: M
authors.lastname: Schmidt
authors.forename: Joachim
authors.initials: J
authors.lastname: Kohl
authors.forename: Matthias W
authors.initials: MW
authors.lastname: Beckmann
authors.forename: Helge
authors.initials: H
authors.lastname: Binder
authors.forename: Inge
authors.initials: I
authors.lastname: Hoffmann
authors.forename: Andreas
authors.initials: A
authors.lastname: Mueller
authors.forename: Ralf
authors.initials: R
authors.lastname: Dittrich
mesh_terms: D000818:Animals; D004958:Estradiol / Q000096:biosynthesis; D005260:Female; D006062:Gonadotropins / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D009929:Organ Size / Q000187:drug effects; D006080:Ovarian Follicle / Q000254:growth & development; D010052:Ovariectomy; D010053:Ovary / Q000187:drug effects / Q000637:transplantation*; D014183:Transplantation, Heterologous; D014599:Uterus / Q000187:drug effects; D014621:Vagina / Q000166:cytology
chemical_list: D006062:Gonadotropins; D004958:Estradiol