title: ESIPT-exhibiting protein probes: a sensitive method for rice proteins detection during starch extraction.
abstract: The 2-(4'-isothiocyanate-2'-hydroxyphenyl)benzoxazole dye was successfully applied as label of rice proteins during the alkaline extraction of starch. Direct fluorescence measurements were used to observe the presence of proteins labelled in different steps of rice starch extraction. The results were compared to those obtained with the well-known biuret colorimetric test. Whereas the colorimetric test indicates the absence of protein after the third extraction step, the fluorescence emission of the conjugate could be observed in all extraction steps. The separation of different rice proteins could also be observed.
authors.affiliation: Universidade Federal do Rio Grande do Sul-Instituto de Química, Laboratório de Instrumentação e Dinâmica Molecular, Av. Bento Gonçalves, 9500. CP 15003 CEP 91501-970, Porto Alegre-RS, Brazil.
authors.forename: Mateus Borba
authors.initials: MB
authors.lastname: Cardoso
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Samios
authors.forename: Nádya Pesce
authors.initials: NP
authors.lastname: da Silveira
authors.forename: Fabiano Severo
authors.initials: FS
authors.lastname: Rodembusch
authors.forename: Valter
authors.initials: V
authors.lastname: Stefani
mesh_terms: D001583:Benzoxazoles / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D017879:Isothiocyanates / Q000737:chemistry*; D015335:Molecular Probes; D015394:Molecular Structure; D012275:Oryza / Q000737:chemistry*; D010940:Plant Proteins / Q000032:analysis* / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D013213:Starch / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: C520959:2-(4'-isothiocyanate-2'-hydroxyphenyl)benzoxazole; D001583:Benzoxazoles; D005456:Fluorescent Dyes; D017879:Isothiocyanates; D015335:Molecular Probes; D010940:Plant Proteins; D013213:Starch