title: Expression changes of apoptotic-related proteins in nerve tissues of rats treated with allyl chloride.
abstract: Allyl chloride (AC) is widely used in industries as raw material and has been reported to produce occupational peripheral neuropathies in man chronically exposure to it. Although many studies have been done addressing to it, the mechanisms still remain unclear. To elucidate the molecular mechanism of neuropathy induced by AC, we measured the contents of glutathione (GSH), Bcl-2, Bax, cytochrome c (CytC) and Caspase-3 in a time-dependent manner by biochemical and quantitative immunoblotting techniques in rats' cerebrum and spinal cord after 3, 6, 9 and 12 weeks of AC intoxication. The results showed that the levels of Bcl-2 of cerebrum and spinal cord significantly (P<0.05) decreased after 9 and 6 weeks of AC intoxication, respectively, while GSH levels decreased after 12 week. However, the levels of Bax, CytC and Caspase-3 significantly (P<0.05) increased both in cerebrum and spinal cord. Bax levels of cerebrum and spinal cord increased after 12 and 9 weeks of AC administration, respectively. The levels of CytC and Caspase-3 also went up after 9 weeks of AC treatment in cerebrum and 9, 6 weeks in spinal cord, respectively. Thus, subchronic exposure to AC affected the expressions of apoptotic-related proteins in the central nervous system (CNS) and peripheral nervous system (PNS) tissues and the time dependent changes of these indexes occurred. The regulatory mechanism of apoptosis might be involved and served as one of mechanisms of toxic neuropathy induced by AC.
authors.affiliation: Institute of Toxicology, Shandong University, 44 West Wenhua Road, Jinan 250012, PR China.
authors.forename: Qing-Shan
authors.initials: QS
authors.lastname: Wang
authors.forename: Fuyong
authors.initials: F
authors.lastname: Song
authors.forename: Xiulan
authors.initials: X
authors.lastname: Zhao
authors.forename: Li-yan
authors.initials: LY
authors.lastname: Hou
authors.forename: Ke-Qin
authors.initials: KQ
authors.lastname: Xie
mesh_terms: D000498:Allyl Compounds / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D045304:Cytochromes c / Q000378:metabolism; D005684:Gait / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D008297:Male; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000187:drug effects* / Q000378:metabolism; D013687:Telencephalon / Q000187:drug effects* / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D000498:Allyl Compounds; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D045304:Cytochromes c; D053148:Caspase 3; D005978:Glutathione; C023698:allyl chloride