[Effect and significance of tetrandrine on epidermal growth factor and its receptor in the lung of congenital diaphragmatic hernia rat model].
OBJECTIVE
To investigate the effect of the traditional Chinese medicine "Tetrandrine"(TET) and its significance on epidermal growth factor (EGF) and its receptor (EGFR) in the lung of nitrofen-induced congenital diaphragmatic hernia (CDH) rat model.


METHODS
Twenty female rats were given maternal administration of a single oral dose (115 mg/rat) of nitrofen to induce CDH at 9.5 days after pregnancy and were divided into normal solution group (NS, n=5), dexamethasone group (Dex, n=5), tetrandrine group (TET, n=5) and Dex+TET group (n=5) at 18.5 days; 4 rats were given edible oil as controls. All fetuses were delivered by cesarean section at 21.5 days. Lung histologic evaluations and EGF, EGFR immunohistochemical staining and image analysis were performed.


RESULTS
CDH was observed in 64 of the 137 rat fetuses (46.7%) in the experimental groups; no CHD was observed in 36 rat fetuses of control group. The lungs of CDH fetuses showed marked hypoplasia in NS group, in contrast to improved mesenchymal differentiation in that of Dex, TET, Dex+TET groups. The expression of EGF was weaker and weaker and that of EGFR was stronger and stronger as following order: NS, TET, DEX, T+D and control groups; showing significant differences between them (P<0.05).


CONCLUSIONS
Prenatal TET administration shows marked improvement in pulmonary hypoplasia through pre-regulating crest-time of EGF expression and up-regulating EGFR expression in the lungs of nitrofen-induced CDH rat model. A combination of TET and Dex would generate evident synergistic effect.
Wang
Yonggang
Y
Department of Pediatric Surgery, West China Hospital, Sichuan University, Chengdu Sichuan, 610041, P. R. China.
Liu
Wenying
W
Lin
Han
H
D000818:Animals; D044182:Benzylisoquinolines / Q000494:pharmacology*; D004195:Disease Models, Animal; D004815:Epidermal Growth Factor / Q000096:biosynthesis; D066246:ErbB Receptors / Q000096:biosynthesis; D005260:Female; D005333:Fetus; D006548:Hernia, Diaphragmatic / Q000378:metabolism*; D065630:Hernias, Diaphragmatic, Congenital; D008168:Lung / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley
D044182:Benzylisoquinolines; C009438:tetrandrine; D004815:Epidermal Growth Factor; D066246:ErbB Receptors