N-glycans of the porcine nematode parasite Ascaris suum are modified with phosphorylcholine and core fucose residues.
In recent years, the glycoconjugates of many parasitic nematodes have attracted interest due to their immunogenic and immunomodulatory nature. Previous studies with the porcine roundworm parasite Ascaris suum have focused on its glycosphingolipids, which were found, in part, to be modified by phosphorylcholine. Using mass spectrometry and western blotting, we have now analyzed the peptide N-glycosidase A-released N-glycans of adults of this species. The presence of hybrid bi- and triantennary N-glycans, some modified by core alpha1,6-fucose and peripheral phosphorylcholine, was demonstrated by LC/electrospray ionization (ESI)-Q-TOF-MS/MS, as was the presence of paucimannosidic N-glycans, some of which carry core alpha1,3-fucose, and oligomannosidic oligosaccharides. Western blotting verified the presence of protein-bound phosphorylcholine and core alpha1,3-fucose, whereas glycosyltransferase assays showed the presence of core alpha1,6-fucosyltransferase and Lewis-type alpha1,3-fucosyltransferase activities. Although, the unusual tri- and tetrafucosylated glycans found in the model nematode Caenorhabditis elegans were not found, the vast majority of the N-glycans found in A. suum represent a subset of those found in C. elegans; thus, our data demonstrate that the latter is an interesting glycobiological model for parasitic nematodes.
Pöltl
Gerald
G
Department für Chemie, Universität für Bodenkultur, Vienna, Austria.
Kerner
Denise
D
Paschinger
Katharina
K
Wilson
Iain B H
IB
D000818:Animals; D001196:Ascariasis / Q000469:parasitology / Q000662:veterinary; D017165:Ascaris suum / Q000737:chemistry* / Q000378:metabolism; D015153:Blotting, Western; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D005643:Fucose / Q000737:chemistry* / Q000378:metabolism; D005647:Fucosyltransferases / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D006858:Hydrofluoric Acid / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D010767:Phosphorylcholine / Q000737:chemistry* / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D013552:Swine; D013553:Swine Diseases / Q000469:parasitology
D011134:Polysaccharides; D010767:Phosphorylcholine; D005643:Fucose; D005647:Fucosyltransferases; D006026:Glycoside Hydrolases; D006858:Hydrofluoric Acid