Detection of pre-neoplastic and neoplastic prostate disease by MALDI profiling of urine.
The heterogeneous progression to the development of prostate cancer (PCa) has precluded effective early detection screens. Existing prostate cancer screening paradigms have relatively poor specificity for cancer relative to other prostate diseases, commonly benign prostatic hyperplasia (BPH). A method for discrimination of BPH, HGPIN, and PCa urine proteome was developed through testing 407 patient samples using matrix assisted laser desorption-mass spectrometry time of flight (MALDI-TOF). Urine samples were adsorbed to reverse phase resin, washed, and the eluant spotted directly for MALDI-TOF analysis of peptides. The processing resolved over 130 verifiable signals of a mass range of 1000-5000 m/z to suggest 71.2% specificity and 67.4% sensitivity in discriminating PCa vs. BPH. Comparing BPH and HGPIN resulted in 73.6% specificity and 69.2% sensitivity. Comparing PCa and HGPIN resulted in 80.8% specificity and 81.0% sensitivity. The high throughput, low-cost assay method developed is amenable for large patient numbers required for supporting biomarker identification.
M'Koma
Amosy E
AE
Department of Urologic Surgery, Vanderbilt-Ingram Cancer Center, Vanderbilt University Medical Center Nashville, TN 37232, USA.
Blum
David L
DL
Norris
Jeremy L
JL
Koyama
Tatsuki
T
Billheimer
Dean
D
Motley
Saundra
S
Ghiassi
Mayshan
M
Ferdowsi
Nika
N
Bhowmick
Indrani
I
Chang
Sam S
SS
Fowke
Jay H
JH
Caprioli
Richard M
RM
Bhowmick
Neil A
NA
D000368:Aged; D006801:Humans; D008297:Male; D008875:Middle Aged; D010455:Peptides / Q000652:urine*; D011230:Precancerous Conditions / Q000175:diagnosis / Q000652:urine; D011470:Prostatic Hyperplasia / Q000175:diagnosis* / Q000652:urine*; D019048:Prostatic Intraepithelial Neoplasia / Q000175:diagnosis* / Q000652:urine*; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000652:urine*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D010455:Peptides