Applications of transcriptional profiling in antibiotics discovery and development.
This chapter will review specific applications of microarray technology and related data analysis strategies in antibacterial research and development. We present examples of microarray applications spanning the entire antibiotics research and development pipeline, from target discovery, assay development, pharmacological evaluation, to compound safety studies. This review emphasizes the utility of microarrays for a systematic evaluation of novel chemistry as antibiotic agents. Transcriptional profiling has revolutionized the process of target elucidation and has the potential to offer substantial guidance in the identification of new targets. Microarrays will continue to be a workhorse of anti-infectives discovery programs ranging from efficacy assessments of antibiotics ('forward pharmacology') to drug safety evaluations ('toxicogenomics').
Fischer
Hans Peter
HP
Genedata AG, Postfach 254, 4016 Basel, Switzerland. Hans-Peter.Fischer@genedata.com
Freiberg
Christoph
C
D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000633:toxicity; D015195:Drug Design; D020869:Gene Expression Profiling; D020411:Oligonucleotide Array Sequence Analysis; D011401:Promoter Regions, Genetic; D040901:Proteomics; D012107:Research Design; D012449:Safety; D013223:Statistics as Topic; D014158:Transcription, Genetic / Q000187:drug effects
D000900:Anti-Bacterial Agents