Neurotrophic effects of polyhydroxylated steroids and steroid glycosides in cultured neuroblastoma cells.
The effects of steroid compounds from Pacific Ocean starfishes were studied using cultured neuroblastoma C-1300 cells. Vital observations and examination of silver-impregnated preparations showed that the test substances in a concentration of 2-10 microM stimulate differentiation and improves survival of neuroblastoma cells under adverse conditions (similarly to neurotrophins). These substances in high concentrations (20-40 microM) had no effect or exhibited cytotoxic activity. The screening test allowed us to select several compounds for further studies of neurotrophic and neuroprotective properties.
Pal'yanova
N V
NV
Institute of Molecular Biology and Biophysics, Siberian Division, Russian Academy of Medical Sciences, Novosibirsk.
Pankova
T M
TM
Starostina
M V
MV
Shtark
M B
MB
Kicha
A A
AA
Ivanchina
N V
NV
Stonik
V A
VA
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006027:Glycosides / Q000494:pharmacology*; D006801:Humans; D009474:Neurons / Q000187:drug effects*; D013215:Starfish / Q000737:chemistry*; D013256:Steroids / Q000494:pharmacology*
D006027:Glycosides; D013256:Steroids