title: The influence of mechanical stimulation on osteocyte apoptosis and bone viability in human trabecular bone.
abstract: It has been shown previously using in vivo and ex vivo animal models, that cyclical mechanical stimulation is capable of maintaining osteocyte viability through the control of apoptotic cell death. Here we have studied the effect of mechanical stimulation on osteocyte viability in human trabecular bone maintained in a 3-D bioreactor system. Bone samples, maintained in the bioreactor system for periods of 3, 7 and 27 days, were subjected to either cyclical mechanical stimulation which engendered a maximum of 3,000 microstrain in a waveform corresponding to physiological jumping exercise for 5 minutes daily or control unloading. Unloading resulted in a decrease in osteocyte viability within 3 days that was accompanied by increased levels of cellular apoptosis. Mechanical stimulation significantly reduced apoptosis (p< or =0.032) and improved the maintenance of osteocyte viability in bone from all patient samples. The percentage Alkaline Phosphatase (ALP) labelled bone surface was significantly increased (p< or =0.05) in response to mechanical stimulation in all samples as was the Bone Formation Rate (BFR/BS) (p=0.005) as determined by calcein label incorporation in the 27-day experiment. These data indicate that in this model system, mechanical stimulation is capable of maintaining osteocyte viability in human bone.
authors.affiliation: Musculoskeletal Tissue Engineering Collaboration, University of Edinburgh Medical School, Edinburgh, UK. val.mann@ed.ac.uk
authors.forename: V
authors.initials: V
authors.lastname: Mann
authors.forename: C
authors.initials: C
authors.lastname: Huber
authors.forename: G
authors.initials: G
authors.lastname: Kogianni
authors.forename: D
authors.initials: D
authors.lastname: Jones
authors.forename: B
authors.initials: B
authors.lastname: Noble
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000469:Alkaline Phosphatase / Q000378:metabolism; D017209:Apoptosis / Q000502:physiology*; D015415:Biomarkers / Q000378:metabolism; D001696:Biomechanical Phenomena; D019149:Bioreactors; D001842:Bone and Bones / Q000166:cytology / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology*; D002478:Cells, Cultured; D005260:Female; D005452:Fluoresceins / Q000378:metabolism; D006801:Humans; D008297:Male; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D008875:Middle Aged; D010011:Osteocytes / Q000166:cytology / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology
chemical_list: D015415:Biomarkers; D005452:Fluoresceins; D000469:Alkaline Phosphatase; C007740:fluorexon