Development of a carbon nanotube paste electrode osmium polymer-mediated biosensor for determination of glucose in alcoholic beverages.
This paper describes a new amperometric biosensor for glucose monitoring. The biosensor is based on the activity of glucose dehydrogenase (GDH) and diaphorase (DI) co-immobilized with NAD(+) into a carbon nanotube paste (CNTP) electrode modified with an osmium functionalized polymer. This mediator was demonstrated to shuttle the electron transfer between the immobilized diaphorase and the CNTP electrode, thus, showing a good electrocatalytic activity towards NADH oxidation at potentials around +0.2V versus Ag|AgCl, where interfering reactions are less prone to occur. The biosensor exhibits a detection limit of 10 micromol L(-1), linearity up to 8 x 10(-4) mol L(-1), a sensitivity of 13.4 microA cm(-2)mmol(-1)L(-1), a good reproducibility (R.S.D. 2.1%, n=6) and a stability of about 1 week when stored dry at 4 degrees C. Finally, the proposed biosensor was applied for the determination of glucose in different samples of sweet wine and validated with a commercial spectrophotometric enzymatic kit.
Antiochia
Riccarda
R
Dipartimento di Scienze Chimiche, Università di Padova, Padova, Italy. riccarda.antiochia@unipd.it <riccarda.antiochia@unipd.it>
Gorton
Lo
L
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005504:Food Analysis / Q000295:instrumentation* / Q000379:methods; D005947:Glucose / Q000032:analysis*; D042843:Glucose 1-Dehydrogenase / Q000737:chemistry*; D008839:Microelectrodes; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009992:Osmium / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D014920:Wine / Q000032:analysis*
D037742:Nanotubes, Carbon; D011108:Polymers; D009992:Osmium; D042843:Glucose 1-Dehydrogenase; D005947:Glucose