Identification of genes from Aspergillus oryzae that are preferentially expressed in membrane-surface liquid culture.
We identified 22 genes from Aspergillus oryzae that are preferentially expressed in membrane-surface liquid culture (MSLC), among which Ser/Thr protein kinase (aopk1) and phosphatase (aoppt) genes were cloned. We also revealed that aopk1 encodes a protein with an N-terminal sequence 150 amino acid residues longer than that predicted from the registered sequence in GenBank.
Feng
Bin
B
Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, 3-1-1 Tsushima-naka, Okayama 700-8530, Japan.
Morita
Masakazu
M
Imanaka
Hiroyuki
H
Imamura
Koreyoshi
K
Nakanishi
Kazuhiro
K
D000595:Amino Acid Sequence; D001236:Aspergillus oryzae / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017346:Protein Serine-Threonine Kinases; D010744:Phosphoric Monoester Hydrolases