Inhibitory effect of quercetin on tryptase and MCP-1 chemokine release, and histidine decarboxylase mRNA transcription by human mast cell-1 cell line.
Mast cells are important in reactions of allergic disease and are also involved in a variety of neuroinflammatory diseases. Mast cells can be immunologically activated by IgE through their Fc receptors, as well as by neuropeptides and cytokines to secrete mediators. Here we used a human mast cell-1 (HMC-1) cell line cultured and treated with a physiological activator, anti-IgE, and a nonphysiological activator, calcium ionophore A23187, for tryptase and MCP-1 generation and transcription of histidine decarboxylase. We used quercetin, a potent antioxidant, cytoprotective and anti-inflammatory compound capable of inhibiting histamine and some cytokines released from several cell types, as an inhibitor of immunological and nonimmunological stimulus for mast cells. In this study quercetin inhibits, in a dose-response manner, tryptase and MCP-1. Moreover, using RT-PCR quercetin inhibited the transcription of histidine decarboxylase, the rate-limiting enzyme responsible for the generation of histamine from histidine, and MCP-1. Our data suggest that quercetin is an important and good candidate for reducing the release of pro-inflammatory mast cell mediators activated by physiological and nonphysiological stimulators.
Castellani
Maria Luisa
ML
Immunology Division, University of Chieti, Italy.
Kempuraj
Duraisamy
D
Frydas
Stavros
S
Theoharides
Theoharis C
TC
Simeonidou
Isaia
I
Conti
Pio
P
Vecchiet
Jacopo
J
D000975:Antioxidants / Q000494:pharmacology*; D015152:Blotting, Northern; D002460:Cell Line; D018932:Chemokine CCL2 / Q000187:drug effects* / Q000378:metabolism; D006640:Histidine Decarboxylase / Q000096:biosynthesis / Q000187:drug effects*; D006801:Humans; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D011794:Quercetin / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000187:drug effects; D053802:Tryptases / Q000187:drug effects* / Q000378:metabolism
D000975:Antioxidants; C111835:CCL2 protein, human; D018932:Chemokine CCL2; D012333:RNA, Messenger; D011794:Quercetin; D053802:Tryptases; D006640:Histidine Decarboxylase