Effect of certain immunosuppressants on non-specific immunity cells in murine corneal grafts: study on early phases after transplantation.
The aim of our study was to evaluate the efficacy of FK506, mycophenolate mofetil (MM) and aminoguanidine (AMG) on infiltration of macrophages (MPHs), neutrophils (NPHs) and dendritic cells (DC) into corneal grafts during the early phases after transplantation (Tx). Tx was performed in mice (C57BL/10 to BALB/c). Therapy included FK506 (0.2 mg/kg), MM (30 mg/kg) or AMG (0.1 g/kg), started at the day of Tx and was injected i.p. daily. Corneas were excised on the third and seventh day after Tx. Immunohistological evaluation using antibodies against MPHs, NPHs and DC was performed and corneal grafts were assessed in the periphery and in central part of the cornea separately. On the third day after Tx, a massive infiltration of MPHs and NPHs into corneal grafts was revealed; the DC infiltration was lower in all treated groups. Treatment with FK506 and MM led to a significant reduction of NPHs in the centers of the grafts, but not of MPHs. In contrast, AMG significantly reduced MPHs migration into allografts on the third day after Tx, whereas NPHs infiltration has not been attenuated. However, immunosuppressants had no influence on the infiltration of DC during early phases after Tx.
Bysterská
P
P
Institute of Pharmacology, First Faculty of Medicine, Charles University, Prague, Czech Republic.
Svozílková
P
P
Farghali
H
H
D000818:Animals; D002452:Cell Count; D003315:Cornea / Q000187:drug effects* / Q000276:immunology / Q000601:surgery; D016039:Corneal Transplantation; D003713:Dendritic Cells / Q000187:drug effects; D005260:Female; D006084:Graft Rejection / Q000276:immunology / Q000517:prevention & control*; D006146:Guanidines / Q000494:pharmacology* / Q000627:therapeutic use; D007166:Immunosuppressive Agents / Q000494:pharmacology* / Q000627:therapeutic use; D008264:Macrophages / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D009173:Mycophenolic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D020556:Neutrophil Infiltration / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects; D016559:Tacrolimus / Q000494:pharmacology* / Q000627:therapeutic use; D013997:Time Factors
D006146:Guanidines; D007166:Immunosuppressive Agents; D009173:Mycophenolic Acid; C004479:pimagedine; D016559:Tacrolimus