P2X7-induced apoptosis decreases by aging in mice myeloblasts.
In the current study, the ability of ATP to promote apoptosis in myeloblasts at different ages was investigated. We have observed that high concentration of extracellular ATP (>1mM), which activates P2X(7) receptor, produced cell shrinkage an increase in the number of events in the sub-G(0)/G(1) region of the cellular cycle and annexin-V/propidium iodide label, which characterizes the apoptotic cell death. In addition, BzATP produced apoptosis, but not ADP and UTP. Gr-1(+) cells express the P2X(7) receptor and oxidized ATP, a specific P2X(7) inhibitor, blocked the ATP-dependent apoptosis. ATP-dependent apoptosis is decreased by aging in myeloblasts of 12 and 22-month-old mice. Furthermore, P2X(7) expression decrease was observed in older mice, explaining apoptosis decrease. This decrease in apoptosis by aging may be related to some diseases in the myelocyte lineage.
Paredes-Gamero
Edgar Julian
EJ
Department of Biophysics, Federal University of São Paulo, R. Botucatu 862, Brazil. jean@biofis.epm.br <jean@biofis.epm.br>
Dreyfuss
Juliana Luporini
JL
Nader
Helena B
HB
Miyamoto Oshiro
Maria Etsuko
ME
Ferreira
Alice Teixeira
AT
D000244:Adenosine Diphosphate / Q000502:physiology; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology / Q000502:physiology; D000345:Affinity Labels / Q000494:pharmacology; D000375:Aging / Q000502:physiology*; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D042381:Granulocyte Precursor Cells / Q000502:physiology*; D006614:Hindlimb; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D058919:Purinergic P2 Receptor Antagonists; D018048:Receptors, Purinergic P2 / Q000502:physiology*; D058486:Receptors, Purinergic P2X7; D014544:Uridine Triphosphate / Q000502:physiology
D000345:Affinity Labels; C546469:P2rx7 protein, mouse; D058919:Purinergic P2 Receptor Antagonists; D018048:Receptors, Purinergic P2; D058486:Receptors, Purinergic P2X7; C017199:2',3'-dialdehyde ATP; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D014544:Uridine Triphosphate