title: Identification of CLUAP1 as a human osteosarcoma tumor-associated antigen recognized by the humoral immune system.
abstract: Since the prognosis of human osteosarcoma in advanced stage remains poor, the development of new and effective therapies including immunotherapy is required. To identify tumor-associated antigens of osteosarcoma applicable to the immunotherapy of this malignancy, we employed the serological analysis of recombinant cDNA expression library (SEREX) technique that defines tumor antigens recognized by the humoral immune system. Screening a cDNA library derived from an osteosarcoma cell line MG63 with sera from osteosarcoma patients identified 43 positive clones, representing 14 distinct antigens. Among them, CLUAP1 (clusterin-associated protein 1) was highly expressed in osteosarcoma tissue samples and cell lines. Overexpression of CLUAP1 was observed in other malignancies including ovarian, colon, and lung cancers. Our results suggest that CLUAP1 may be useful as a prognostic/diagnostic marker and/or for a target of immunotherapy of osteosarcoma.
authors.affiliation: Division of Immunoregulation, Institute for Genetic Medicine, Hokkaido University, Sapporo 060-0815, Japan.
authors.forename: Hisamitsu
authors.initials: H
authors.lastname: Ishikura
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ikeda
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Abe
authors.forename: Takayuki
authors.initials: T
authors.lastname: Ohkuri
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Hiraga
authors.forename: Kazuo
authors.initials: K
authors.lastname: Isu
authors.forename: Tomohide
authors.initials: T
authors.lastname: Tsukahara
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Sato
authors.forename: Hidemitsu
authors.initials: H
authors.lastname: Kitamura
authors.forename: Norimasa
authors.initials: N
authors.lastname: Iwasaki
authors.forename: Naoki
authors.initials: N
authors.lastname: Takeda
authors.forename: Akio
authors.initials: A
authors.lastname: Minami
authors.forename: Takashi
authors.initials: T
authors.lastname: Nishimura
mesh_terms: D000293:Adolescent; D000951:Antigens, Neoplasm / Q000378:metabolism / Q000502:physiology*; D045744:Cell Line, Tumor; D002648:Child; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D006801:Humans; D007107:Immune System / Q000378:metabolism*; D008297:Male; D012516:Osteosarcoma / Q000276:immunology / Q000378:metabolism* / Q000628:therapy; D011379:Prognosis; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014018:Tissue Distribution
chemical_list: D000951:Antigens, Neoplasm; C518614:CLUAP1 protein, human; D018076:DNA, Complementary; D012333:RNA, Messenger; D011994:Recombinant Proteins