title: Polysaccharide/polynucleotide complexes. Part 6: complementary-strand-induced release of single-stranded DNA bound in the schizophyllan complex.
abstract: Spectroscopic properties of single-stranded DNA/schizophyllan ternary complexes (ss-DNA2s-SPG), induced by addition of either complementary or noncomplementary strands, have been investigated. The addition of the complementary strands to ss-DNA2s-SPG induced the quick release of the bound ss-DNA to the complementary strands (both DNA and RNA), whereas the ternary complex was unaffected upon addition of noncomplementary strands. Our experiments imply that SPG has complexation properties indispensable to the gene carriers. As far as we know, there is no report on exploitation of such nonviral gene carriers that can accomplish an intelligent release of the bound ss-DNA toward the complementary strands. We believe, therefore, that SPG, a natural and neutral polysaccharide, has a great potential to become a new ss-DNA carrier.
authors.affiliation: Department of Chemical Processes and Environments, Faculty of Environmental Engineering, The University of Kitakyushu, 1-1 Hibikino, Wakamatsu-ku, Kitakyushu, Fukuoka 808-0135, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Koumoto
authors.forename: Masami
authors.initials: M
authors.lastname: Mizu
authors.forename: Kazuo
authors.initials: K
authors.lastname: Sakurai
authors.forename: Toyoki
authors.initials: T
authors.lastname: Kunitake
authors.forename: Seiji
authors.initials: S
authors.lastname: Shinkai
mesh_terms: D018076:DNA, Complementary / Q000737:chemistry / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000378:metabolism; D011119:Polynucleotides / Q000737:chemistry / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D012566:Sizofiran / Q000737:chemistry / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D004277:DNA, Single-Stranded; D011119:Polynucleotides; D011134:Polysaccharides; D012566:Sizofiran