Characterization of tachykinin-related peptides from different insect species on Drosophila tachykinin receptor-expressing cell line.
Drosophila tachykinin receptor, a neurokinin receptor cloned from the fruit fly Drosophila melanogaster, is a G-protein-coupled receptor, which upon activation by a peptide agonist induces a transient increase in the concentration of intracellular calcium. The functional assay based on aequorin-derived luminescence triggered by receptor-mediated changes in Ca(2+) levels was used to examine and compare the effect of tachykinin-related peptides from different species. Among the endogenous Drosophila peptides, Drm-TK I induced the strongest calcium response. The most potent tachykinin-related peptides from Leucophaea maderae, Locusta migratoria, and Calliphora vomitoria, were partial agonists at the Drosophila tachykinin receptor.
Fichna
Jakub
J
Laboratory of Biomolecular Chemistry, Medical University of Lodz, Mazowiecka 6/8, 92-215 Lodz, Poland.
Poels
Jeroen
J
Broeck
Jozef Vanden
JV
Janecka
Anna
A
D000818:Animals; D002118:Calcium / Q000032:analysis / Q000378:metabolism; D002460:Cell Line; D029721:Drosophila Proteins; D010455:Peptides / Q000494:pharmacology; D018039:Receptors, Tachykinin / Q000502:physiology*; D013045:Species Specificity; D015320:Tachykinins / Q000737:chemistry / Q000494:pharmacology*
D029721:Drosophila Proteins; D010455:Peptides; D018039:Receptors, Tachykinin; D015320:Tachykinins; D002118:Calcium