title: Ischemia promotes calpain-mediated degradation of p120-catenin in SH-SY5Y cells.
abstract: p120-catenin contributes to the cadherin-mediated adhesion and aggregation of cells. mu-Calpain was activated and p120-catenin was degraded after 36 h of ischemia in differentiated SH-SY5Y cells. Calpain inhibitors Cbz-Val-Phe-H (MDL28170, 20 microM) and N-acetyl-leucyl-leucyl-norleucinal (ALLN, 20 microM) increased the levels of dephosphorylated p120-catenin, aggregation, and cell survival as detected by reduced LDH release in ischemic cells. However, a proteasome inhibitor lactacystin had no such effects. This is the first report of the calpain-mediated degradation of p120-catenin and an association between the level of dephosphorylated p120-catenin and cell aggregation in ischemic neuronal cells.
authors.affiliation: Department of Forensic Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ohno
authors.forename: Koichi
authors.initials: K
authors.lastname: Uemura
authors.forename: Kaori
authors.initials: K
authors.lastname: Shintani-Ishida
authors.forename: Mihoko
authors.initials: M
authors.lastname: Nakamura
authors.forename: Mitsushi
authors.initials: M
authors.lastname: Inomata
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Yoshida
mesh_terms: D000111:Acetylcysteine / Q000031:analogs & derivatives / Q000494:pharmacology; D002154:Calpain / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051177:Catenins; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002449:Cell Aggregation / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D004151:Dipeptides / Q000494:pharmacology; D006801:Humans; D007511:Ischemia / Q000503:physiopathology*; D007976:Leupeptins / Q000494:pharmacology; D009447:Neuroblastoma / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism*; D000095002:Delta Catenin
chemical_list: D051177:Catenins; D015815:Cell Adhesion Molecules; D004151:Dipeptides; D007976:Leupeptins; D010750:Phosphoproteins; C063261:acetylleucyl-leucyl-norleucinal; C067713:lactacystin; D002154:Calpain; C058076:calpain inhibitor III; D000111:Acetylcysteine; D000095002:Delta Catenin; C000722347:CTNND1 protein, human