title: Time-resolved fluoroimmunoassay for ractopamine in swine tissue.
abstract: This study describes the development and validation of a time-resolved fluoroimmunoassay (TR-FIA) for screening ractopamine (RAC) in swine tissue. The method is based on the direct competitive-type immunoassay using europium-labeled anti-RAC monoclonal antibody as a tracer and RAC-ovalbumin as a solid-phase antigen. When RAC was spiked at levels of 1-10 microg kg(-1), recoveries ranged from 88.2 to 118.5% for swine liver and muscle with coefficients of variation from 7.1 to 20.5%. The detection limit was 0.1 microg kg(-1). The proposed TR-FIA method was applied to the determination of RAC in an actual residue study and the applicability was confirmed by liquid chromatography-tandem mass spectrometry.
authors.affiliation: Department of Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100094, China.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Shen
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Yao
authors.forename: Weimin
authors.initials: W
authors.lastname: Shi
authors.forename: Yibing
authors.initials: Y
authors.lastname: Liu
authors.forename: Suxia
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D015200:Fluoroimmunoassay / Q000379:methods*; D008099:Liver / Q000276:immunology; D009132:Muscles / Q000276:immunology; D010627:Phenethylamines / Q000032:analysis* / Q000276:immunology*; D013552:Swine; D013997:Time Factors
chemical_list: D010627:Phenethylamines; C060897:ractopamine