title: Effects of murine endotoxemia on lymphocyte subsets and clearance of staphylococcal pulmonary infection.
abstract: In a model of staphylococcal pneumonia initiated during systemic endotoxemia in BALB/c mice, a significant reduction of the number of circulating CD4+ and CD8+ T-lymphocytes, B-lymphocytes, and NK cells, as well as lung-resident total T- and CD4+ T-lymphocytes was demonstrated. Staphylococcus aureus exposure only induced a similar decrease of lymphocyte subsets in the blood. However, the number of lung-resident total T- and CD4+ T-lymphocytes was increased. More viable bacteria were recovered from the lungs of S. aureus-infected mice than from those animals previously treated with lipopolysaccharide (LPS) followed by a staphylococcal challenge. These results indicate that LPS-induced reduction in the number of circulating lymphocyte subsets and lung-resident total T- and CD4+ T-lymphocytes do not increase susceptibility to staphylococcal respiratory infection. Moreover, LPS challenge prior to S. aureus exposure significantly improves clearance of the bacteria in the lung.
authors.affiliation: 3rd Department of Infectious and Tropical Diseases, First Faculty of Medicine, Charles University, 180 81 Prague, Czechia. michal.holub@lf1.cuni.cz
authors.forename: M
authors.initials: M
authors.lastname: Holub
authors.forename: D A
authors.initials: DA
authors.lastname: Lawrence
authors.forename: T K
authors.initials: TK
authors.lastname: Mondal
mesh_terms: D000818:Animals; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D019446:Endotoxemia / Q000276:immunology*; D005260:Female; D008070:Lipopolysaccharides / Q000276:immunology*; D008168:Lung / Q000276:immunology / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D011023:Pneumonia, Staphylococcal / Q000276:immunology* / Q000382:microbiology
chemical_list: D008070:Lipopolysaccharides