title: A microsatellite linkage map of Barramundi, Lates calcarifer.
abstract: Barramundi (Lates calcarifer) is an important farmed marine food fish species. Its compact genome (approximately 700 Mb) is among the smallest genomes of food fish species. We established a first-generation genetic linkage map of Barramundi with a mapping panel containing three parents (two males and one female) and 93 progeny. A total of 240 microsatellite markers were mapped into 24 linkage groups. Among these markers, 10 were located in ESTs and known genes. The total lengths of the female and male maps were 873.8 and 414.5 cM with an average marker spacing of 6.20 and 4.70 cM, respectively. Comparing the flanking sequences of the 240 Barramundi microsatellites with the assembled whole-genome sequences of Tetraodon nigrovidiris revealed 55 homologous sequences located in 19 of the 21 chromosomes of T. nigrovidiris. The map will not only enable the mapping of quantitative trait loci, but also provide new resources for understanding the evolution of fish genomes.
authors.affiliation: Molecular Population Genetics Group, Temasek Life Sciences Laboratory, National University of Singapore, 117604 Singapore, Republic of Singapore.
authors.forename: Chun Ming
authors.initials: CM
authors.lastname: Wang
authors.forename: Ze Yuan
authors.initials: ZY
authors.lastname: Zhu
authors.forename: Loong Chueng
authors.initials: LC
authors.lastname: Lo
authors.forename: Felicia
authors.initials: F
authors.lastname: Feng
authors.forename: Grace
authors.initials: G
authors.lastname: Lin
authors.forename: Wen Tong
authors.initials: WT
authors.lastname: Yang
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Gen Hua
authors.initials: GH
authors.lastname: Yue
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D002875:Chromosomes; D004777:Environment; D005260:Female; D005399:Fishes / Q000235:genetics*; D008040:Genetic Linkage; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D019655:Quantitative Trait, Heritable; D011995:Recombination, Genetic / Q000235:genetics; D012727:Sex Characteristics