[Fluorescently labeled differentiating myelopeptide-4: specific binding to and penetration into target cells].
Myelopeptide-4 (MP-4) (Phe-Arg-Pro-Arg-Ile-Met-Thr-Pro), inducing the terminal differentiation of HL-60 leukemia cells, was labeled with fluorescein isothiocyanate. The specific binding of this modified peptide to the surface of HL-60 cells and its ability to penetrate into the cells were studied. It was shown by cytometry and confocal microscopy to be bound on the HL-60 cell surface, to penetrate into their cytoplasm, and finally to concentrate around the cell nucleus. These phenomena are probably necessary for the exhibition of MP-4 differentiating activity.
Gur'ianov
S A
SA
Kirilina
E A
EA
Khaĭdukov
S V
SV
Suvorov
N I
NI
Molotkovskaia
I M
IM
Mikhaĭlova
A A
AA
D002454:Cell Differentiation / Q000187:drug effects*; D003593:Cytoplasm / Q000378:metabolism*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D018922:HL-60 Cells; D006801:Humans; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D021381:Protein Transport / Q000187:drug effects
D005456:Fluorescent Dyes; D009842:Oligopeptides; C502954:phenylalanyl-arginyl-prolyl-arginyl-isoleucyl-methionyl-threonyl-proline; D016650:Fluorescein-5-isothiocyanate