Time-resolved fluoroimmunoassay for ractopamine in swine tissue.
This study describes the development and validation of a time-resolved fluoroimmunoassay (TR-FIA) for screening ractopamine (RAC) in swine tissue. The method is based on the direct competitive-type immunoassay using europium-labeled anti-RAC monoclonal antibody as a tracer and RAC-ovalbumin as a solid-phase antigen. When RAC was spiked at levels of 1-10 microg kg(-1), recoveries ranged from 88.2 to 118.5% for swine liver and muscle with coefficients of variation from 7.1 to 20.5%. The detection limit was 0.1 microg kg(-1). The proposed TR-FIA method was applied to the determination of RAC in an actual residue study and the applicability was confirmed by liquid chromatography-tandem mass spectrometry.
Shen
Jianzhong
J
Department of Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100094, China.
Zhang
Zhen
Z
Yao
Yan
Y
Shi
Weimin
W
Liu
Yibing
Y
Zhang
Suxia
S
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D015200:Fluoroimmunoassay / Q000379:methods*; D008099:Liver / Q000276:immunology; D009132:Muscles / Q000276:immunology; D010627:Phenethylamines / Q000032:analysis* / Q000276:immunology*; D013552:Swine; D013997:Time Factors
D010627:Phenethylamines; C060897:ractopamine