[Relationship between human age and mtDNA 4977 deletions in human blood cells].
OBJECTIVE
To study the relationship between human age and mtDNA 4977 deletions in human blood cells.


METHODS
According to Anderson's sequence, two sets of primers were designed. Human mtDNA 4977 deletions in blood samples from 110 healthy human individuals in different age groups were examined by using Real time quantitative PCR.


RESULTS
The mtDNA 4977 deletions could be observed unless the age was under 23 years old. The older the people were, the easier the deletion could be observed.


CONCLUSIONS
mtDNA 4977 deletions in human blood cells were correlated with human ages in some extent.
Li
Cheng-Tao
CT
Institute of Forensic Sciences, Ministry of Justice, P. R. China, Shanghai 200063, China. qzjdzx@163.com
Li
Li
L
Liu
Yan
Y
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000375:Aging / Q000235:genetics*; D000704:Analysis of Variance; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D004252:DNA Mutational Analysis; D017931:DNA Primers / Q000235:genetics; D004272:DNA, Mitochondrial / Q000097:blood / Q000235:genetics*; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D017384:Sequence Deletion; D055815:Young Adult
D017931:DNA Primers; D004272:DNA, Mitochondrial; D005456:Fluorescent Dyes