title: Calibration of matrix-assisted laser desorption/ionization time-of-flight peptide mass fingerprinting spectra.
abstract: This chapter describes a number of aspects important for calibration of matrix-assisted laser desorption/ionization time-of-flight spectra prior to peptide mass fingerprinting searches. Both multipoint internal calibration and mass defect-based calibration is illustrated. The chapter describes how potential internal calibrants, like tryptic autodigest peptides and keratin-related peptides, can be identified and used for high-precision calibration. Furthermore, the construction of project/user-specific lists of potential calibrants is illustrated.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense.
authors.forename: Karin
authors.initials: K
authors.lastname: Hjernø
authors.forename: Peter
authors.initials: P
authors.lastname: Højrup
mesh_terms: D002138:Calibration; D007633:Keratins / Q000737:chemistry; D008970:Molecular Weight; D010449:Peptide Mapping / Q000379:methods*; D012984:Software; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D007633:Keratins