[Inhibition of IFN-gamma receptor signaling by hepatitis C virus non-structural protein NS4B].
The function of NS4B is incompletely understood. The aim of the study is to understand the influence of NS4B on anti-viral response. After cell line stably expressing NS4B established, the influence of IFN-alpha of different concentration on VSV was studied using plaque assay; cell expression profiling caused by NS4B was studied using DNA microarray, and the IFNGR1 fluorescence intensity was analyzed. Our data showed that HCV-NS4B could suppress immuno-associated gene expression, in particular, IFN-gamma receptor signal transduction-related genes. Taken together, NS4B could play some roles in HCV resistance to IFN therapy.
Zheng
Yi
Y
Department of Life Sciences, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China. zhengyi1205@126.com
Luo
Hai-Bo
HB
Gao
Bo
B
Huang
Lai-Qiang
LQ
D024882:Drug Resistance, Viral; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006526:Hepatitis C / Q000188:drug therapy; D006801:Humans; D007371:Interferon-gamma / Q000627:therapeutic use; D020411:Oligonucleotide Array Sequence Analysis; D017471:Receptors, Interferon / Q000037:antagonists & inhibitors* / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology*; D017361:Viral Nonstructural Proteins / Q000502:physiology*; D000096743:Interferon gamma Receptor
D007371:Interferon-gamma; D017471:Receptors, Interferon; D017361:Viral Nonstructural Proteins; D000096743:Interferon gamma Receptor; C080107:NS4B protein, flavivirus