title: Identification of new p63 targets in human keratinocytes.
abstract: p63 is a transcription factor involved in the development of ectodermal tissues, including limb, skin and, in general, multilayered epithelia. We identified both activated and repressed genes in human keratinocytes via gene expression profiling of p63-depleted cells and validated 21 new primary targets by RT-PCR and ChIP location analysis. The p63 isoforms differentially activate or repress selected promoters. ChIPs in primary keratinocytes indicate that p63 targets are generally shared with p53, but some are p63-specific. Several growth suppressors are among repressed genes. The newly identified genes belong to pathways of growth and differentiation and are regulated in HaCaT differentiation and in stratification of human skin.
authors.affiliation: Dipartimento di Scienze Biomolecolari e Biotecnologie, U. di Milano, Milano, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Testoni
authors.forename: Serena
authors.initials: S
authors.lastname: Borrelli
authors.forename: Elena
authors.initials: E
authors.lastname: Tenedini
authors.forename: Daniela
authors.initials: D
authors.lastname: Alotto
authors.forename: Carlotta
authors.initials: C
authors.lastname: Castagnoli
authors.forename: Stefano
authors.initials: S
authors.lastname: Piccolo
authors.forename: Enrico
authors.initials: E
authors.lastname: Tagliafico
authors.forename: Sergio
authors.initials: S
authors.lastname: Ferrari
authors.forename: M Alessandra
authors.initials: MA
authors.lastname: Viganò
authors.forename: Roberto
authors.initials: R
authors.lastname: Mantovani
mesh_terms: D002454:Cell Differentiation; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000235:genetics*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D012867:Skin / Q000166:cytology; D013379:Substrate Specificity; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; C115051:TP63 protein, human; D015534:Trans-Activators; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins