Controlling tissue microenvironments: biomimetics, transport phenomena, and reacting systems.
The reconstruction of tissues ex vivo and production of cells capable of maintaining a stable performance for extended time periods in sufficient quantity for synthetic or therapeutic purposes are primary objectives of tissue engineering. The ability to characterize and manipulate the cellular microenvironment is critical for successful implementation of such cell-based bioengineered systems. As a result, knowledge of fundamental biomimetics, transport phenomena, and reaction engineering concepts is essential to system design and development. Once the requirements of a specific tissue microenvironment are understood, the biomimetic system specifications can be identified and a design implemented. Utilization of novel membrane systems that are engineered to possess unique transport and reactive features is one successful approach presented here. The limited availability of tissue or cells for these systems dictates the need for microscale reactors. A capstone illustration based on cellular therapy for type 1 diabetes mellitus via encapsulation techniques is presented as a representative example of this approach, to stress the importance of integrated systems.
Fisher
Robert J
RJ
Department of Chemical Engineering, Building 66, Room 446, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Peattie
Robert A
RA
D001693:Biological Transport, Active; D032701:Biomimetics / Q000295:instrumentation* / Q000379:methods / Q000639:trends; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods / Q000639:trends; D004780:Environment, Controlled; D004867:Equipment Design; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods / Q000639:trends; D008904:Miniaturization; D046509:Tissue Culture Techniques / Q000295:instrumentation* / Q000379:methods / Q000639:trends; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods / Q000639:trends