Effect of short-term outlet obstruction on rat bladder nerve density and contractility.
1 The present study was designed to investigate the relationship between innervation density and contractile responses to field stimulation and exogenous agonists at early time points after induction of bladder outlet obstruction (BOO) in rats. 2 When compared with sham-operated animals, 1, 3 and 7 days of BOO were associated with a 75%, 80% and 90% increase of bladder weight. Field stimulation caused a frequency-dependent increase in force of contraction. The force of contraction was reduced at each frequency in BOO rats with the greatest decrease after 1 day and a gradual but incomplete recovery thereafter. In contrast, contractile responses to ATP, carbachol and KCl were markedly reduced after 1 day of BOO and fully recovered after 7 days. The neurofilament staining was not altered by 1 day of BOO, but gradually decreased with increasing duration of BOO reaching the lowest levels after 7 days. 3 We conclude that impaired cellular contractility seems to underlie the early reductions of field stimulation-induced contraction, possibly reflecting surgical trauma of the tissue. However, at later time points a reduced nerve density, possibly reflecting a partial denervation, appears to be the main reason for impaired contractile response to field stimulation.
Barendrecht
M M
MM
Department of Pharmacology and Pharmacotherapy, Academic Medical Center, Amsterdam, The Netherlands.
Chichester
P
P
Michel
M C
MC
Levin
R M
RM
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D066298:In Vitro Techniques; D008026:Ligation; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology*; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014521:Urethra / Q000187:drug effects; D001743:Urinary Bladder / Q000187:drug effects / Q000294:innervation* / Q000503:physiopathology*; D001748:Urinary Bladder Neck Obstruction / Q000139:chemically induced / Q000473:pathology* / Q000503:physiopathology*
D011189:Potassium Chloride; D000255:Adenosine Triphosphate; D002217:Carbachol