title: CE frontal analysis based on simultaneous UV and contactless conductivity detection: a general setup for studying noncovalent interactions.
abstract: CE frontal analysis (CE-FA) has been established as a powerful tool to study noncovalent interactions between macromolecules and small molecules such as drug substances or pharmaceutical excipients. However, when using traditional commercial CE instrumentation, a serious drawback is related to the fact that only UV-active compounds can be studied. In recent years, contactless conductivity detection has become an attractive alternative to UV detection in CE due to its high versatility. In this study, we combine contactless conductivity detection and UV detection in a highly versatile setup for profiling noncovalent interactions between low-molecular-weight molecules and macromolecules. In the case of molecules having a chromophore the setup allows determination of binding constants using two independent detectors. The new contactless conductivity detection cell is compatible with commercial CE instrumentation and is therefore easily implemented in any analysis laboratory with CE expertise.
authors.affiliation: Department of Pharmaceutics and Analytical Chemistry, The Danish University of Pharmaceutical Sciences, Copenhagen, Denmark. hj@dfuni.dk
authors.forename: Henrik
authors.initials: H
authors.lastname: Jensen
authors.forename: Jesper
authors.initials: J
authors.lastname: Ostergaard
authors.forename: Anne E
authors.initials: AE
authors.lastname: Thomsen
authors.forename: Steen H
authors.initials: SH
authors.lastname: Hansen
mesh_terms: D002021:Buffers; D002414:Cations, Monovalent; D016264:Dextran Sulfate / Q000737:chemistry; D004553:Electric Conductivity; D004566:Electrodes; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D007546:Isoquinolines / Q000737:chemistry*; D011189:Potassium Chloride / Q000737:chemistry; D011726:Pyridinium Compounds / Q000737:chemistry*; D014466:Ultraviolet Rays
chemical_list: D002021:Buffers; D002414:Cations, Monovalent; D007546:Isoquinolines; D011726:Pyridinium Compounds; D011189:Potassium Chloride; D016264:Dextran Sulfate