The ntrC gene of Agrobacterium tumefaciens C58 controls glutamine synthetase (GSII) activity, growth on nitrate and chromosomal but not Ti-encoded arginine catabolism pathways.
The ntrC locus of Agrobacterium tumefaciens C58 has been cloned using the Azorhizobium sesbaniae ORS571 ntrC gene as a DNA hybridization probe. Transposon Tn5 mutagenesis of the cloned ntrC locus was carried out and one Tn5 insertion within the region of highest DNA homology with A. sesbaniae ORS571 ntrC was used for gene replacement of the wild-type C58 ntrC gene. The A. tumefaciens ntrC::Tn5 mutant was found to be unable to grow on nitrate as sole nitrogen (N) source, to lack glutamine synthetase (GSII) activity and to be unable to use arginine (or ornithine) as sole N source, unless the Ti-encoded arginine catabolism pathway was induced with small amounts of nopaline. Thus the A. tumefaciens ntrC regulatory gene is essential for (transcriptional) activation of the GSII and nitrate reductase genes, as well as for the chromosomal but not the Ti-borne arginine catabolism pathways.
Rossbach
S
S
Max-Planck-Institut für Züchtungsforschung, Abteilung Genetische Grundlagen der Pflanzenzüchtung, D-5000, Köln 30, Federal Republic of Germany.
Schell
J
J
de Bruijn
F J
FJ
D016960:Agrobacterium tumefaciens / Q000201:enzymology / Q000235:genetics*; D001120:Arginine / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D005798:Genes, Bacterial / Q000235:genetics*; D005974:Glutamate-Ammonia Ligase / Q000235:genetics*; D009566:Nitrates / Q000378:metabolism
D001426:Bacterial Proteins; D009566:Nitrates; C083573:ntrC protein, Rhizobium; D001120:Arginine; D005974:Glutamate-Ammonia Ligase