Procedure-induced inflammation and endothelial cell activation in an artificially ventilated and circulated porcine double-lung model.
Systemic inflammation is induced during extracorporeal circulation, resulting in an increased bleeding tendency and endothelial cell activation. Lungs from seven piglets were perfused by autologous blood in an extracorporeal circuit, where the lungs and the left atrium were attached to polyvinyl chloride (PVC) tubings and the blood circulated by a roller pump. The trachea was intubated and attached to a ventilator. The lungs maintained good gas exchange, despite a slight increase in lactate levels. Plasma tPA increased slightly over time, suggesting endothelial cell activation. Activation of inflammatory systems was reflected in increased levels of plasma interleukin (IL)-6 and IL-10. A model for the study of lung endothelial activation during extracorporeal circulation has been shown to be reproducible. The lung tissue was shown to be capable of gas exchange and activation of endothelial cells and procedure-induced inflammation were noted.
Adrian
Katrin
K
Institute of Clinical Sciences, Sahlgrenska Academy at Göteborg University, Göteborg, Sweden. katrin.adrian@pediat.gu.se
Skogby
Maria
M
Gatzinsky
Vladimir
V
Friberg
Lars-Göran
LG
Mellgren
Karin
K
D000818:Animals; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D004867:Equipment Design; D005112:Extracorporeal Circulation / Q000009:adverse effects*; D005260:Female; D007249:Inflammation / Q000517:prevention & control; D016753:Interleukin-10 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D007442:Intubation, Intratracheal; D008168:Lung / Q000502:physiology*; D023421:Models, Animal; D011659:Pulmonary Gas Exchange; D012121:Respiration, Artificial / Q000379:methods*; D018709:Statistics, Nonparametric; D013552:Swine
D015850:Interleukin-6; D016753:Interleukin-10