Multiple interacting oncogenes on the 8p11-p12 amplicon in human breast cancer.
The 8p11-p12 genomic region is amplified in 15% of breast cancers and harbors several candidate oncogenes. However, functional evidence for a transforming role for these genes is lacking. We identified 21 genes from this region as potential oncogenes based on statistical association between copy number and expression. We further showed that three of these genes (LSM1, BAG4, and C8orf4) induce transformed phenotypes when overexpressed in MCF-10A cells, and overexpression of these genes in combination influences the growth factor independence phenotype and the ability of the cells to grow under anchorage-independent conditions. Thus, LSM1, BAG4, and C8orf4 are breast cancer oncogenes that can work in combination to influence the transformed phenotype in human mammary epithelial cells.
Yang
Zeng Quan
ZQ
Breast Cancer Program, University of Michigan School of Medicine, Ann Arbor, Michigan 48201, USA.
Streicher
Katie L
KL
Ray
Michael E
ME
Abrams
Judith
J
Ethier
Stephen P
SP
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D001943:Breast Neoplasms / Q000235:genetics*; D002455:Cell Division; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8; D003470:Culture Media; D005260:Female; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D009363:Neoplasm Proteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D009857:Oncogenes; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D048868:Adaptor Proteins, Signal Transducing; C116906:BAG4 protein, human; D003470:Culture Media; C464824:LSM1 protein, human; D009363:Neoplasm Proteins; D011518:Proto-Oncogene Proteins; D016601:RNA-Binding Proteins; C418741:TCIM protein, human