title: Oxidized LDL specifically promotes the initiation of monocyte invasion during transendothelial migration with upregulated PECAM-1 and downregulated VE-cadherin on endothelial junctions.
abstract: It is poorly understood how oxidized LDL (oxLDL) promotes monocyte dynamics in transendothelial migration (TEM) in atherogenesis. We developed an in vitro 3D-live-single cell TEM assay system with subendothelial oxLDL embedded in ultra-thin collagen gels, mimicking subendothelial oxLDL accumulation in vivo. With dividing monocyte dynamics into three stages (1: adhesion on endothelium, 2: invasion and 3: complete transmigration below endothelium), we analyzed the stage transition dynamics of individual living human monocytes. OxLDL did not enhance initial monocyte adhesion to endothelium (stage 1), but it specifically primed adherent monocytes to start invasion (stage 1-->2). Once invasion started, it had no effect thereafter on monocyte stage transition (stage 2-->3). OxLDL upregulated PECAM-1 and downregulated VE-cadherin on endothelial junctions without monocyte addition, both of which could promote monocyte entry by enhanced homophilic binding to monocyte PECAM-1, and by disrupted junctional barrier, respectively. Meanwhile, monocyte speed at neither locomotion on endothelium (stage 1) nor subendothelial migration (stage 3) was altered by oxLDL. These data indicate that before monocyte adhesion, endothelial junctions changed their conformation to more monocyte-acceptable state in response to oxLDL, resulting the stage-specific promotion of monocyte TEM (stage 1-->2; initiation of invasion) with no enhancement of its initial adhesion or migration speed.
authors.affiliation: Department of Physiology, Kawasaki Medical School, 577 Matsushima, Kurashiki, Okayama 701-0192, Japan. khashimo@med.kawasaki-m.ac.jp
authors.forename: Ken
authors.initials: K
authors.lastname: Hashimoto
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Kataoka
authors.forename: Emi
authors.initials: E
authors.lastname: Nakamura
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Tsujioka
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Kajiya
mesh_terms: D015703:Antigens, CD / Q000378:metabolism*; D050197:Atherosclerosis; D015820:Cadherins / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods; D002465:Cell Movement / Q000502:physiology; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte / Q000502:physiology*; D015536:Down-Regulation; D042783:Endothelial Cells; D006801:Humans; D021621:Imaging, Three-Dimensional; D007365:Intercellular Junctions / Q000502:physiology; D008077:Lipoproteins, LDL / Q000502:physiology*; D009000:Monocytes / Q000502:physiology*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism*; D014471:Umbilical Veins / Q000166:cytology / Q000502:physiology; D015854:Up-Regulation; D000099152:Cadherin 5
chemical_list: D015703:Antigens, CD; D015820:Cadherins; D008077:Lipoproteins, LDL; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D000099152:Cadherin 5; C099252:oxidized low density lipoprotein