title: Bone marrow mononuclear cells stimulate angiogenesis when transplanted into surgically induced fibrocollagenous tunnels: results from a canine ischemic hindlimb model.
abstract: Progenitor cell transplantation has been considered as a potential angiogenesis therapy for the ischemic hindlimb. In this work we performed an ischemic hindlimb model in dogs. We ligated the middle sacra and the external right iliac arteries. After 7 days, the femoral artery was ligated and removed, and three Silastic tubes were inserted into the gracilis muscle to create fibrocollagenous tunnels. After Silastic implantation, we administered saline or granulocyte colony stimulating factor (G-CSF) subcutaneously daily during 5 days. Fourteen days after device positioning we transplanted bone marrow mononuclear cells (BMMC) into the tunnels previously formed by Silastic tube reaction. Twenty-eight days later, contrasted angiographies were performed and angiographic scores were calculated. Also, vessels and endothelial cells and proliferating cells were identified by immunochemistry of muscle sections. Results demonstrated that BMMC transplantation enriched by G-CSF administration significantly stimulates angiogenesis in the ischemic hindlimb, and more than BMMC transplantation alone. Transplantation of progenitor cells in an appropriate extracellular matrix is a potential therapy for hindlimb ischemia.
authors.affiliation: Department of Microsurgery, Centro Médico Nacional "20 de Noviembre", I.S.S.S.T.E., Mexico City, Mexico.
authors.forename: Luis
authors.initials: L
authors.lastname: Padilla
authors.forename: Edgar
authors.initials: E
authors.lastname: Krötzsch
authors.forename: Anabel S
authors.initials: AS
authors.lastname: De La Garza
authors.forename: Siegfried
authors.initials: S
authors.lastname: Figueroa
authors.forename: Juan
authors.initials: J
authors.lastname: Rodriguez-Trejo
authors.forename: Gerardo
authors.initials: G
authors.lastname: Avila
authors.forename: Paul
authors.initials: P
authors.lastname: Schalch
authors.forename: Ignacio
authors.initials: I
authors.lastname: Escotto
authors.forename: Germán
authors.initials: G
authors.lastname: Glennie
authors.forename: Fernando
authors.initials: F
authors.lastname: Villegas
authors.forename: Mauricio
authors.initials: M
authors.lastname: Di Silvio
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000008:administration & dosage*; D000792:Angiography; D000818:Animals; D003094:Collagen / Q000032:analysis / Q000235:genetics; D004195:Disease Models, Animal; D004285:Dogs; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage*; D006614:Hindlimb / Q000098:blood supply*; D007511:Ischemia / Q000000981:diagnostic imaging / Q000601:surgery*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000637:transplantation*; D018919:Neovascularization, Physiologic; D018709:Statistics, Nonparametric
chemical_list: D043925:Angiogenesis Inducing Agents; D016179:Granulocyte Colony-Stimulating Factor; D003094:Collagen