title: Convenient method for monitoring Abeta aggregation by quartz-crystal microbalance.
abstract: We have found that a quartz-crystal microbalance is a facile and useful tool for detecting the specific aggregation of the amyloid-beta peptides responsible for Alzheimer's disease. This method greatly reduces the time and samples (corresponds to 1 Hz frequency decrease per 30 pg mass increase on a 4.9 mm(2) electrode) that are needed in the aggregation assay, and is also applicable to molecular interactions between smaller amyloid-beta fragment peptides such as the pentapeptide KLVFF.
authors.affiliation: Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1 Funabashi, Chiba 274-8510, Japan. okuno@phar.toho-u.ac.jp
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Okuno
authors.forename: Kanae
authors.initials: K
authors.lastname: Mori
authors.forename: Tomofumi
authors.initials: T
authors.lastname: Jitsukawa
authors.forename: Hiromi
authors.initials: H
authors.lastname: Inoue
authors.forename: Shinya
authors.initials: S
authors.lastname: Chiba
mesh_terms: D000327:Adsorption; D000544:Alzheimer Disease; D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000032:analysis* / Q000378:metabolism; D019281:Dimerization; D004566:Electrodes; D006801:Humans; D008722:Methods; D008970:Molecular Weight; D011791:Quartz
chemical_list: D016229:Amyloid beta-Peptides; D011791:Quartz