Protein expression profiling of the shrimp cellular response to white spot syndrome virus infection.
To better understand the pathogenesis of white spot syndrome virus (WSSV) and to determine which cell pathways might be affected after WSSV infection, two-dimensional gel electrophoresis (2-DE) was used to produce protein expression profiles from samples taken at 48 h post-infection (hpi) from the stomachs of Litopenaeus vannamei (also called Penaeus vannamei) that were either specific pathogen free or else infected with WSSV. Seventy-five protein spots that consistently showed either a marked change (>50%) in accumulated levels or else were highly expressed throughout the course of WSSV infection were selected for further study. After in-gel trypsin digestion followed by LC-nanoESI-MS/MS, bioinformatics databases were searched for matches. A total of 53 proteins were identified, with functions that included energy production, calcium homeostasis, nucleic acid synthesis, signaling/communication, oxygen carrier/transportation, and SUMO-related modification. 2-DE results were shown to be consistent with relative EST database data from a previously developed EST database of two Penaeus monodon cDNA libraries. For seven selected genes, 2-DE and EST data were also compared with transcriptional time-course RT-PCR data. This study is the first global analysis of differentially expressed proteins in WSSV-infected shrimp, and in addition to increasing our understanding of the molecular pathogenesis of this virus-associated shrimp disease, the results presented here should be useful both for identifying potential biomarkers and for developing antiviral measures.
Wang
Hao-Ching
HC
Institute of Biochemical Sciences, National Taiwan University, Taipei 106, Taiwan, ROC.
Wang
Han-Ching
HC
Leu
Jiann-Horng
JH
Kou
Guang-Hsiung
GH
Wang
Andrew H-J
AH
Lo
Chu-Fang
CF
D000818:Animals; D004266:DNA Virus Infections / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D015180:Electrophoresis, Gel, Two-Dimensional; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D033561:Penaeidae / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D040901:Proteomics / Q000379:methods*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013047:Specific Pathogen-Free Organisms; D046848:White spot syndrome virus 1 / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger