title: Recognition characteristics of monoclonal antibodies that are cross-reactive with gangliosides and lipooligosaccharide from Campylobacter jejuni strains associated with Guillain-Barré and Fisher syndromes.
abstract: The enteropathogen Campylobacter jejuni has the ability to synthesize glycan structures that are similar to mammalian gangliosides within the core component of its lipooligosaccharide (LOS). Exposure to ganglioside mimics in some individuals results in the production of autoantibodies that deleteriously attack nerve surface gangliosides, precipitating the onset of Guillain-Barré and Fisher syndromes (GBS and FS). We have characterized the interaction of four monoclonal antibodies (mAbs), established by sensitization of mice with LOS isolated from GBS- and FS-associated C. jejuni strains, with chemoenzymatically synthesized gangliooligosaccharides. Surface plasmon resonance (SPR) measurements demonstrate that three of the mAbs interact specifically with derivatives corresponding to their targeted gangliosides, with dissociation constants ranging from 10 to 20 microM. Antibody binding to the gangliooligosaccharides was probed by saturation transfer difference (STD) NMR spectroscopy. STD signals, resulting from antibody/oligosaccharide interaction, were observed for each of the four mAbs. In two cases, differential saturation transfer rates to oligosaccharide resonances enabled detailed epitope mapping. The binding of GD1a-S-Phe with GB1 is characterized by close association of the immunoglobulin with sites that are distributed over several residues of the oligosaccharide. This contrasts sharply with the profile observed for the binding of both GD3-S-Phe and GT1a-S-Phe with FS1. The close antigenic contacts in these ganglioside derivatives are confined to the N-acetylmannosaminyl portion of the terminal N-acetylneuraminic acid (NeuAc) residue of the disialosyl moiety. Our characterization of FS1 provides insight, at an atomic level, into how a single antigenic determinant presented by the LOS of C. jejuni can give rise to antibodies with binding promiscuity to [alphaNeuAc-(2-8)-alphaNeuAc]-bound epitopes and demonstrates why sera from FS patients have antibodies that are often reactive with more than one disialylated ganglioside.
authors.affiliation: Institute for Biological Sciences, National Research Council Canada, Ottawa, Ontario K1A 0R6, Canada.
authors.forename: R Scott
authors.initials: RS
authors.lastname: Houliston
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Yuki
authors.forename: Tomoko
authors.initials: T
authors.lastname: Hirama
authors.forename: Nam H
authors.initials: NH
authors.lastname: Khieu
authors.forename: Jean-Robert
authors.initials: JR
authors.lastname: Brisson
authors.forename: Michel
authors.initials: M
authors.lastname: Gilbert
authors.forename: Harold C
authors.initials: HC
authors.lastname: Jarrell
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000737:chemistry / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology*; D000942:Antigens, Bacterial / Q000737:chemistry* / Q000276:immunology; D001666:Binding Sites, Antibody; D002169:Campylobacter Infections / Q000276:immunology; D016123:Campylobacter jejuni / Q000737:chemistry / Q000276:immunology*; D003429:Cross Reactions; D005732:Gangliosides / Q000737:chemistry / Q000276:immunology*; D020275:Guillain-Barre Syndrome / Q000276:immunology; D006801:Humans; D007700:Kinetics; D008024:Ligands; D008070:Lipopolysaccharides / Q000737:chemistry / Q000276:immunology*; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D019846:Miller Fisher Syndrome / Q000276:immunology; D020349:Surface Plasmon Resonance
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D005732:Gangliosides; D008024:Ligands; D008070:Lipopolysaccharides; C023023:lipid-linked oligosaccharides