Pseudomonas aeruginosa inducing rice resistance against Rhizoctonia solani: production of salicylic acid and peroxidases.
Three isolates of Pseudomonas aeruginosa were used for seed treatment of rice; all showed plant growth promoting activity and induced systemic resistance in rice against Rhizoctonia solani G5 and increased seed yield. Production of salicylic acid (Sal) by P. aeruginosa both in vitro and in vivo was quantified with high performance liquid chromatography. All three isolates produced more Sal in King's B broth than in induced roots. Using a split root system, more Sal accumulated in root tissues of bacterized site than in distant roots on the opposite site of the root system after 1 d, but this difference decreased after 3 d. Sal concentration 0-200 g/L showed no inhibition of mycelial growth of R. solani in vitro, while at > or =300 g/L it inhibited it. P. aeruginosa-pretreated rice plants challenged inoculation with R. solani (as pathogen), an additional increase in the accumulation of peroxidase was observed. Three pathogenesis-related peroxidases in induced rice plants were detected; molar mass of these purified peroxidases was 28, 36 and 47 kDa. Purified peroxidase showed antifungal activity against phytopathogenic fungi R. solani, Pyricularia oryzae and Helminthosporium oryzae.
Saikia
R
R
Laboratory of Applied Mycology, Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi 221 005, India. ratulsaikia@yahoo.com
Kumar
R
R
Arora
D K
DK
Gogoi
D K
DK
Azad
P
P
D000898:Antibiosis / Q000502:physiology; D000935:Antifungal Agents / Q000378:metabolism*; D007113:Immunity, Innate; D012275:Oryza / Q000201:enzymology* / Q000382:microbiology; D010544:Peroxidases / Q000378:metabolism; D010572:Pest Control, Biological / Q000379:methods; D010935:Plant Diseases / Q000382:microbiology*; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D012232:Rhizoctonia / Q000472:pathogenicity*; D020156:Salicylic Acid / Q000378:metabolism
D000935:Antifungal Agents; D010544:Peroxidases; D020156:Salicylic Acid