GC-MS quantification of suspected volatile allergens in fragrances. 2. Data treatment strategies and method performances.
The performances of the GC-MS determination of suspected allergens in fragrance concentrates have been investigated. The limit of quantification was experimentally determined (10 mg/L), and the variability was investigated for three different data treatment strategies: (1) two columns and three quantification ions; (2) two columns and one quantification ion; and (3) one column and three quantification ions. The first strategy best minimizes the risk of determination bias due to coelutions. This risk was evaluated by calculating the probability of coeluting a suspected allergen with perfume constituents exhibiting ions in common. For hydroxycitronellal, when using a two-column strategy, this may statistically occur more than once every 36 analyses for one ion or once every 144 analyses for three ions in common.
Bassereau
Maud
M
Takasago, 23-25 Avenue De la Mare, F-95310 Saint Ouen l'Aumône, France.
Chaintreau
Alain
A
Duperrex
Stéphanie
S
Joulain
Daniel
D
Leijs
Hans
H
Loesing
Gerd
G
Owen
Neil
N
Sherlock
Alan
A
Schippa
Christine
C
Thorel
Pierre-Jean
PJ
Vey
Matthias
M
D000485:Allergens / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D010476:Perfume / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D023422:Skin Irritancy Tests; D014835:Volatilization
D000485:Allergens; D010476:Perfume