Gene polymorphisms associated with reduced hepatic expression of porcine mannan-binding lectin C.
Previous studies showed that low expression of mannan-binding lectin C (MBL-C) in pigs was not due to single-nucleotide polymorphisms (SNPs) in the coding region of pig MBL2. In these studies, we compared the 5' flanking regions of porcine MBL1 (1907 bp) and MBL2 (1880 bp) in normal and diseased pigs with low or high hepatic expression of MBL2. Hepatic expression of MBL-C was very low in all pigs submitted for postmortem diagnosis. In various European pig breeds, a G(-1081)A substitution was linked to very low hepatic MBL-C expression, and was more frequent in diseased pigs. A C(-251)T substitution with less influence on MBL-C expression was more common in various breeds but was not associated with disease. MBL2 polymorphisms were associated with some disease groups and with the presence of some etiologic agents. These findings indicate that some promoter polymorphisms impair MBL-C expression in pigs and may increase their susceptibility to disease.
Lillie
Brandon N
BN
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ont., Canada N1G 2W1.
Keirstead
Natalie D
ND
Squires
E James
EJ
Hayes
M Anthony
MA
D000818:Animals; D001483:Base Sequence; D020022:Genetic Predisposition to Disease; D008099:Liver / Q000378:metabolism*; D037601:Mannose-Binding Lectin / Q000096:biosynthesis* / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D013552:Swine / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D013553:Swine Diseases / Q000235:genetics* / Q000276:immunology
D037601:Mannose-Binding Lectin