title: Increase in annexin V-positive B cells expressing LILRB1/ILT2/CD85j in malaria.
abstract: The outcome of a Plasmodium falciparum infection differs greatly between patients, ranging from an asymptomatic carrier status to the most severe characteristics influenced by activating and inhibiting immune factors. The inhibitory leukocyte immunoglobulin-like receptor (LILRB1/CD85j) plays an important role in the immune response as regulator of cytotoxic T cells and of premature activation and clonal expansion of B cells. To investigate its role in malaria, we analyzed blood samples from malaria patients by cytometric analysis. We found a similar expression pattern of CD85j on PBMC in both patients and healthy children. However, malaria patients presented significantly more CD85j+ CD19+ B cells, which also bound annexin V an indicator of early cell death. We compared the plasma levels of several cytokines, since it was speculated that CD85j expression influences cytokine release. Production of inflammatory cytokines was significantly increased in severe malaria cases. We suggest that in malaria, dying B cells contribute to the overwhelming cytokine release and the impairment of the immune memory.
authors.affiliation: Department of Parasitology, Institute for Tropical Medicine, University of Tübingen, Wilhelmstr. 27, 72074 Tübingen, Germany.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Kalmbach
authors.forename: Angelica B W
authors.initials: AB
authors.lastname: Boldt
authors.forename: Rolf
authors.initials: R
authors.lastname: Fendel
authors.forename: Benjamin
authors.initials: B
authors.lastname: Mordmüller
authors.forename: Peter G
authors.initials: PG
authors.lastname: Kremsner
authors.forename: Jürgen F J
authors.initials: JF
authors.lastname: Kun
mesh_terms: D017304:Annexin A5 / Q000097:blood*; D015703:Antigens, CD / Q000378:metabolism*; D001402:B-Lymphocytes / Q000378:metabolism*; D006801:Humans; D000075362:Leukocyte Immunoglobulin-like Receptor B1; D016778:Malaria, Falciparum / Q000097:blood*; D011971:Receptors, Immunologic / Q000378:metabolism*
chemical_list: D017304:Annexin A5; D015703:Antigens, CD; C105299:LILRB1 protein, human; D000075362:Leukocyte Immunoglobulin-like Receptor B1; D011971:Receptors, Immunologic