Cloning of the Bz2 locus of Zea mays using the transposable element Ds as a gene tag.
The Bz2 locus of Zea mays has been cloned, utilizing the presence of the transposable element Dissociation (Ds) at the locus as a gene flag. The Ds element inserted in the bz2-m allele was identified among many members of the Ac/Ds family in a Southern blot analysis of a population segregating for bz2-m and Bz2. After cloning a DNA fragment from the bz2-m allele, sequences flanking the Ds insertion were shown to be Bz2-specific and were used to isolate a homologous fragment from a wild-type Bz2 line. The Ds insertion in the bz2-m allele was found to be a Ds2 element identical to the Ds insertion in adh1-2F11.
Theres
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N
Institut für Genetik, Universität zu Köln, D-5000 Köln 41, Federal Republic of Germany.
Scheele
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T
Starlinger
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P
D003001:Cloning, Molecular; D004251:DNA Transposable Elements / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D003313:Zea mays / Q000235:genetics*
D004251:DNA Transposable Elements; D010940:Plant Proteins; C076971:BZ2 protein, Zea mays