Decrease in extracellular collagen crosslinking after NMR magnetic field application in skin fibroblasts.
Although biological effects of electromagnetic fields were investigated intensively, there is still no agreement on the significance of their effects. The underlying mechanisms and therapeutic importance are still mostly unknown too. In this study, primary cultures of human dermal fibroblasts were exposed to magnetic field at nuclear magnetic resonance (NMR) conditions for in total 5 days and 4 h/day. Among the investigated parameters were: cell proliferation rate, cell morphology, total protein concentration as well as content of skin-specific collagen types I, III, IV. NMR exposure induced distinct changes both in cellular and extracellular components. The extracellular matrix (ECM) of NMR-exposed cells had less cross-linked collagen. In particular, the increase of collagen of the soluble fraction was at 17.2 +/- 2.9% for type I, 27.0 +/- 1.86% for type III, 17.3 +/- 1.46% for type IV (N = 6). In the absence of resonance frequency, the effects of magnetic field on ECM were less profound.
Digel
I
I
Department of Biomedical Engineering, Laboratory of Cell Biophysics, Aachen University of Applied Sciences, Ginsterweg 1, 52428, Juelich, Germany. digel@fh-aachen.de
Kurulgan
E
E
Linder
Pt
P
Kayser
P
P
Porst
D
D
Braem
G J
GJ
Zerlin
K
K
Artmann
G M
GM
Artmann
A Temiz
AT
D001834:Body Water / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000032:analysis; D049109:Cell Proliferation; D002478:Cells, Cultured; D003094:Collagen / Q000032:analysis / Q000378:metabolism*; D003432:Cross-Linking Reagents / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005109:Extracellular Matrix / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007525:Isoelectric Focusing; D009682:Magnetic Resonance Spectroscopy / Q000009:adverse effects*; D020543:Proteome
D015815:Cell Adhesion Molecules; D003432:Cross-Linking Reagents; D003598:Cytoskeletal Proteins; D020543:Proteome; D003094:Collagen