title: Genetically selected stem cells from human adipose tissue express cardiac markers.
abstract: In the present study, the potential of human adipose-derived stem cells to differentiate into cells with characteristics of cardiomyocytes was investigated. Adipose tissue-derived stem cells (ADSCs) were transduced with two different lentiviral vectors simultaneously: (1) a lentiviral vector expressing eGFP controlled by the Nkx2.5 promoter and (2) a lentiviral vector expressing DsRed2 controlled by the myosin light chain-2v promoter (MLC-2v). Nkx2.5-eGFP and MLC-2v-DsRed2 dual positive cells were isolated by FACS. Immunostaining and RT-PCR analysis of the dual positive cells revealed that these cells are positive for Nkx2.5, cardiac troponin I, and L-type calcium channel alpha-1c subunit. Electrophysiology studies demonstrated the presence of functional voltage-dependent calcium and potassium channels. These observations confirm that cardiac progenitor cells can be isolated and enriched from human adipose-derived stem cells using lentiviral selection, and they might represent a new source for cell therapy for myocardial infarction and heart failure.
authors.affiliation: Department of Molecular Pathology, University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Bai
authors.forename: Kai
authors.initials: K
authors.lastname: Pinkernell
authors.forename: Yao-Hua
authors.initials: YH
authors.lastname: Song
authors.forename: Christoph
authors.initials: C
authors.lastname: Nabzdyk
authors.forename: Jakob
authors.initials: J
authors.lastname: Reiser
authors.forename: Eckhard
authors.initials: E
authors.lastname: Alt
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D020746:Calcium Channels, L-Type / Q000502:physiology; D002454:Cell Differentiation; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000096:biosynthesis; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D008164:Luminescent Proteins / Q000096:biosynthesis; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism; D024642:Potassium Channels, Voltage-Gated / Q000502:physiology; D013234:Stem Cells / Q000378:metabolism*; D014161:Transduction, Genetic
chemical_list: D020746:Calcium Channels, L-Type; D008164:Luminescent Proteins; D024642:Potassium Channels, Voltage-Gated; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins