title: Histological investigation of diagnostically challenging tubule profiles in advanced chronic progressive nephropathy (CPN) in the fischer 344 RaT.
abstract: Recently, guidelines were suggested for discriminating proliferative-appearing tubule profiles encountered in advanced spontaneous chronic progressive nephropathy (CPN) of rats, from hyperplastic precursors of renal tubule tumors (Hard and Seely, 2005). These recommendations were based on histological evaluation of a large number of cases of severe to end-stage CPN in male F344 rats from 8 separate 2-year carcinogenicity studies held in the Archives of the National Toxicology Program, NIEHS. This work has now been extended to characterize the various lesions further, mainly by serial sectioning to track their origin and fate within the adjacent renal tissue, but also by applying special staining procedures such as immunohistochemical assessment of proliferative activity, as well as fluorescence microscopy, to seek further differences from atypical tubule hyperplasia. The results obtained from these additional investigations support the contention that certain tubule profiles with a misleading proliferative appearance, sometimes found in advanced CPN, should be distinguished from preneoplastic tubule foci, and regarded as components of the nephropathy process.
authors.affiliation: National Toxicology Program Archives, Research Triangle Park, North Carolina 27709, USA.
authors.forename: Gordon C
authors.initials: GC
authors.lastname: Hard
authors.forename: John Curtis
authors.initials: JC
authors.lastname: Seely
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002908:Chronic Disease; D004247:DNA / Q000096:biosynthesis / Q000235:genetics; D005109:Extracellular Matrix / Q000473:pathology; D006965:Hyperplasia / Q000473:pathology; D007150:Immunohistochemistry; D007674:Kidney Diseases / Q000473:pathology*; D007684:Kidney Tubules / Q000473:pathology*; D008297:Male; D008856:Microscopy, Fluorescence; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D004247:DNA