Synthesis and evaluation of novel chromogenic aminopeptidase substrates for microorganism detection and identification.
The amides 8a-e and 10a-c were prepared as chromogenic aminopeptidase substrates. A range of microorganisms were grown in the presence of these compounds and coloured colonies were produced in several cases after addition of acetic acid-thus giving potential methods for the detection of aminopeptidase activity and for microorganism identification.
James
Arthur L
AL
School of Applied Sciences, Northumbria University, Newcastle upon Tyne, NE1 8ST, UK.
Perry
John D
JD
Rigby
Annette
A
Stanforth
Stephen P
SP
D000577:Amides / Q000138:chemical synthesis*; D000626:Aminopeptidases / Q000378:metabolism*; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*; D002863:Chromogenic Compounds / Q000138:chemical synthesis*; D003116:Color; D006090:Gram-Negative Bacteria; D006094:Gram-Positive Bacteria; D006868:Hydrolysis; D013329:Structure-Activity Relationship
D000577:Amides; D002863:Chromogenic Compounds; D000626:Aminopeptidases