Analysis of biodegradation of copolymer dermis substitutes in the dorsal skinfold chamber of balb/c mice.
PEGT/PBT-block-copolymer dermis substitutes were inserted into dorsal skinfold chambers of balb/c mice (n=36). Scaffolding matrices with 3 different pore diameters (pore diameter: <75 micro m, 75-212 micro m and 250-300 micro m) were analyzed on days 7, 14, and 21 post implantation by scanning electron and light microscopy. The quantification of matrix fragmentation was performed using image-analytical software analySIS(R). The fragmentation rate in scaffolding matrices with a pore size of < 75 micro m was observed to be higher than in matrices of larger pore sizes. Image-analytical evaluation over 21 days revealed a reduction of the copolymer matrix by approximately 32% for the <75 micro m matrices, 23% for the 75-212 micro m matrices and 18% for the matrices, where pore size ranged between 250 micro m and 300 micro m. Twenty-one days after implantation, the matrix pores of 75-212 micro m and 250-300 micro m scaffolds were totally filled by vascularized fibrous tissue. Contrarily, an increased formation of foreign-body giant cells was observed in matrices with pore size <75 micro m. The pore size of the scaffolding PEGT/PBT dermis substitutes affects their degradative behaviour in vivo.
Ring
A
A
Department of Surgery, Trauma Center, University Hospital Bergmannsheil, Ruhr University Bochum, Buerkle-de-la-Camp Platz 1, 44789 Bochum, Germany.
Goertz
O
O
Steinstraesser
L
L
Kuhnen
C
C
Schmitz
I
I
Muhr
G
G
Steinau
H U
HU
Langer
Stefan
S
D000818:Animals; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D011091:Polyesters / Q000627:therapeutic use*; D011092:Polyethylene Glycols / Q000627:therapeutic use*; D017768:Skin, Artificial; D023822:Tissue Engineering; D016896:Treatment Outcome
C493255:PEGT-PBT copolymer; D011091:Polyesters; D011092:Polyethylene Glycols