title: The alliance for cellular signaling plasmid collection: a flexible resource for protein localization studies and signaling pathway analysis.
abstract: Cellular responses to inputs that vary both temporally and spatially are determined by complex relationships between the components of cell signaling networks. Analysis of these relationships requires access to a wide range of experimental reagents and techniques, including the ability to express the protein components of the model cells in a variety of contexts. As part of the Alliance for Cellular Signaling, we developed a robust method for cloning large numbers of signaling ORFs into Gateway entry vectors, and we created a wide range of compatible expression platforms for proteomics applications. To date, we have generated over 3000 plasmids that are available to the scientific community via the American Type Culture Collection. We have established a website at www.signaling-gateway.org/data/plasmid/ that allows users to browse, search, and blast Alliance for Cellular Signaling plasmids. The collection primarily contains murine signaling ORFs with an emphasis on kinases and G protein signaling genes. Here we describe the cloning, databasing, and application of this proteomics resource for large scale subcellular localization screens in mammalian cell lines.
authors.affiliation: Alliance for Cellular Signaling, California Institute of Technology, CA 94305, USA.
authors.forename: Joelle R
authors.initials: JR
authors.lastname: Zavzavadjian
authors.forename: Sam
authors.initials: S
authors.lastname: Couture
authors.forename: Wei Sun
authors.initials: WS
authors.lastname: Park
authors.forename: James
authors.initials: J
authors.lastname: Whalen
authors.forename: Stephen
authors.initials: S
authors.lastname: Lyon
authors.forename: Genie
authors.initials: G
authors.lastname: Lee
authors.forename: Eileen
authors.initials: E
authors.lastname: Fung
authors.forename: Qingli
authors.initials: Q
authors.lastname: Mi
authors.forename: Jamie
authors.initials: J
authors.lastname: Liu
authors.forename: Estelle
authors.initials: E
authors.lastname: Wall
authors.forename: Leah
authors.initials: L
authors.lastname: Santat
authors.forename: Kavitha
authors.initials: K
authors.lastname: Dhandapani
authors.forename: Christine
authors.initials: C
authors.lastname: Kivork
authors.forename: Adrienne
authors.initials: A
authors.lastname: Driver
authors.forename: Xiaocui
authors.initials: X
authors.lastname: Zhu
authors.forename: Mi Sook
authors.initials: MS
authors.lastname: Chang
authors.forename: Baljinder
authors.initials: B
authors.lastname: Randhawa
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Gehrig
authors.forename: Heather
authors.initials: H
authors.lastname: Bryan
authors.forename: Mary
authors.initials: M
authors.lastname: Verghese
authors.forename: Andreia
authors.initials: A
authors.lastname: Maer
authors.forename: Brian
authors.initials: B
authors.lastname: Saunders
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Ning
authors.forename: Shankar
authors.initials: S
authors.lastname: Subramaniam
authors.forename: Tobias
authors.initials: T
authors.lastname: Meyer
authors.forename: Melvin I
authors.initials: MI
authors.lastname: Simon
authors.forename: Nancy
authors.initials: N
authors.lastname: O'Rourke
authors.forename: Grischa
authors.initials: G
authors.lastname: Chandy
authors.forename: Iain D C
authors.initials: ID
authors.lastname: Fraser
mesh_terms: D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D016208:Databases, Factual; D051379:Mice; D016366:Open Reading Frames / Q000235:genetics; D010957:Plasmids; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D015398:Signal Transduction
chemical_list: D018076:DNA, Complementary; D011494:Protein Kinases