Tetraploid somatic hybrids of potato (Solanum tuberosum L.) obtained from diploid breeding lines.
Intraspecific somatic hybrids between 16 different diploid breeding lines of Solanum tuberosum L. were produced by PEG-induced fusion. Manually selected heterokaryons were cultured in a Millicells-CM using a post-fusion protoplast mixture. Plants were regenerated from calli derived from heterokaryons obtained from 10 out of 38 combinations of diploid lines. Of the tested putative somatic hybrids, 14.2% were diploid, 72.8% were tetraploid and 13% pentaploid. The DNA amplification pattern obtained with RAPD or semi-random primers confirmed that 6 fusion combinations were hybrids. In most cases, the morphological traits were intermediate to those of the diploid fusion partners. About 23.0% of the tested somatic hybrids showed variation in their morphology. Of the tested somatic hybrids, 78.0% flowered and 86.0% tuberized. The cytoplasm of 9 diploid lines and 6 somatic hybrid combinations was analysed. Two of the diploid lines had W/S chloroplasts and alpha or epsilon mitochondria; the remainder contained T chloroplasts and beta mitochondria. All the analysed somatic hybrids carried T chloroplasts and beta mitochondria.
Przetakiewicz
Jarosław
J
Plant Transformation and Cell Engineering Department, Plant Breeding and Acclimatization Institute, Radzików, Błonie, Poland.
Nadolska-Orczyk
Anna
A
Kuć
Dominik
D
Orczyk
Wacław
W
D001947:Breeding; D002467:Cell Nucleus / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism; D032461:Chromosomes, Plant / Q000235:genetics; D004272:DNA, Mitochondrial / Q000378:metabolism; D004171:Diploidy; D006824:Hybridization, Genetic; D008928:Mitochondria / Q000378:metabolism; D010641:Phenotype; D018517:Plant Roots / Q000166:cytology; D012150:Polymorphism, Restriction Fragment Length; D011123:Polyploidy; D011523:Protoplasts / Q000378:metabolism; D019655:Quantitative Trait, Heritable; D012038:Regeneration; D011198:Solanum tuberosum / Q000166:cytology / Q000235:genetics* / Q000502:physiology
D004272:DNA, Mitochondrial