title: Cell distribution profiles in three-dimensional scaffolds with inverted-colloidal-crystal geometry: modeling and experimental investigations.
abstract: Limited ingrowth of stromal cells is observed when a three-dimensionally ordered scaffold possessing inverted-colloidal-crystal geometry is used to culture adherent cells. In this work, a computational model explaining, as well as predicting, experimental cell distributions is developed. It incorporates a modified Contois cell-growth model that includes the effects of nutrient saturation, competitive product inhibition, and cell-contact inhibition to describe the scaffold-cell system. Our results agree with the hypothesis that the rapid growth of cells on the surface of the scaffold depletes the nutrient supply to the core, resulting in the preferential growth on the exterior of the scaffold. When the cells are cultured in a scaffold subjected to a uniform velocity field, they penetrate to a greater extent into the scaffold core. Alternative seeding and culture strategies are suggested and evaluated.
authors.affiliation: Department of Chemical Engineering, University of Michigan, 3074 HH Dow Building, 2300 Hayward, Ann Arbor, MI 48109, USA.
authors.forename: Sachin
authors.initials: S
authors.lastname: Shanbhag
authors.forename: Shaopeng
authors.initials: S
authors.lastname: Wang
authors.forename: Nicholas A
authors.initials: NA
authors.lastname: Kotov
mesh_terms: D001672:Biocompatible Materials; D001709:Biotechnology / Q000379:methods*; D001854:Bone Marrow Cells / Q000166:cytology; D002450:Cell Communication; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods*; D003102:Colloids / Q000737:chemistry*; D003460:Crystallization; D003470:Culture Media / Q000737:chemistry; D004058:Diffusion; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D008954:Models, Biological; D017154:Stromal Cells / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D003102:Colloids; D003470:Culture Media