Hyperbaric oxygenation attenuates renal ischemia-reperfusion injury in rats.
BACKGROUND
Oxygen-derived free radicals play an important role in ischemia-reperfusion injury (IR). Hyperbaric oxygenation (HO) decreases free radical production. The aim of this study was to determine the effect of HO treatment on renal ischemia-reperfusion injury in rats.


METHODS
Rats were divided into four groups. All groups underwent right nephrectomy. Group I served as the control group; group II had left renal ischemia-reperfusion; group III was pretreated with HO; and group IV, ischemia-reperfusion and HO pretreatment. Tissue malondialdehyde (MDA) and glutathione (GSH) levels were measured, and histopathologic damage scored.


RESULTS
HO pretreatment significantly decreased tissue MDA levels and histopathologic scores among rats with IR. There was an increased GSH in HO-pretreated rats with IR; however, the difference was not significant.


CONCLUSIONS
HO prior to ischemia displayed a beneficial effect on renal IR by reducing oxygen radical peroxidation of lipid membranes.
Gurer
A
A
Department of Surgery, Yeditepe University Hospitals, Istanbul, Turkey.
Ozdogan
M
M
Gomceli
I
I
Demirag
A
A
Gulbahar
O
O
Arikok
T
T
Kulacoglu
H
H
Dundar
K
K
Ozlem
N
N
D000818:Animals; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D006931:Hyperbaric Oxygenation; D008297:Male; D009336:Necrosis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012079:Renal Circulation; D015427:Reperfusion Injury / Q000097:blood / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*
D003404:Creatinine