Anti-seizure efficacy of nimodipine in pentylenetetrazole and kainic acid combined seizure models in mice.
The present study aimed at establishing two models of experimental seizures by combination treatment with subconvulsive doses of PTZ and kainic acid in adult male mice and evaluating the modulatory role of cerebroselective dihydropyridine calcium channel blocker, nimodipine. The CD50 +/- SEM value for PTZ was found to be 20.00 +/- 0.92 mg/kg, ip in kainic acid (administered at per se subconvulsive dose of 1.00 mg/kg, ip) pretreated mice while CD50 +/- SEM value for kainic acid was found to be 0.30 +/- 0.08 mg/kg, ip in PTZ (administered at per se subconvulsive dose of 30.00 mg/kg, ip) pretreated mice. Nimodipine (5.00 mg/kg, ip) significantly protected the mice from seizure in both of the combination in vivo seizure models. The results suggested synergistic interaction between PTZ and kainic acid at subconvulsive dose combination while the protective efficacy of nimodipine suggested the role of calcium ion as an important mediator for the genesis of seizures.
Shitak
R
R
Department of Pharmacology, Post Graduate Institute of Medical Education and Research, Chandigarh 160 012.
Sahai
A K
AK
Hota
D
D
Chakrabarti
A
A
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D003292:Convulsants / Q000633:toxicity*; D004195:Disease Models, Animal; D018690:Excitatory Amino Acid Agonists / Q000633:toxicity*; D007608:Kainic Acid / Q000819:agonists / Q000633:toxicity*; D051379:Mice; D009553:Nimodipine / Q000494:pharmacology*; D010433:Pentylenetetrazole / Q000819:agonists / Q000633:toxicity*; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*
D002121:Calcium Channel Blockers; D003292:Convulsants; D018690:Excitatory Amino Acid Agonists; D009553:Nimodipine; D007608:Kainic Acid; D002118:Calcium; D010433:Pentylenetetrazole