Cloning and characterization of 40S ribosomal protein S4 gene from Culex pipiens pallens.
The 40S ribosomal protein S4 gene (RPS4) has been cloned from Culex pipiens pallens. An open reading frame (ORF) of 789 bp was found to encode a putative 262 amino acid protein. The deduced amino acid sequence shares 96% and 91% identity with RPS4 genes from Aedes and Anopheles respectively. Transcript expression of RPS4 was determined by real-time PCR in all life stages of deltamethrin-susceptible and -resistant strains. The results demonstrated that this gene is expressed at all developmental stages. Meanwhile, in pupae and adults, RPS4 is overexpressed in deltamethrin-resistant strain than in -susceptible strain. Our data for the first time suggests that increased expression of the RPS4 gene may play some role in the development of deltamethrin resistance in C. pipiens pallens.
Hu
Xiaobang
X
Department of Pathogen Biology, Nanjing Medical University; Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, PR China.
Wang
Weijie
W
Zhang
Donghui
D
Jiao
Jianhua
J
Tan
Wenbin
W
Sun
Yan
Y
Ma
Lei
L
Zhu
Changliang
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003465:Culex / Q000235:genetics* / Q000254:growth & development; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D007305:Insecticide Resistance / Q000235:genetics; D007306:Insecticides / Q000494:pharmacology; D008297:Male; D008969:Molecular Sequence Data; D009570:Nitriles / Q000494:pharmacology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011722:Pyrethrins / Q000494:pharmacology; D012269:Ribosomal Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; D012269:Ribosomal Proteins; C031798:ribosomal protein S4; C017180:decamethrin