title: [Scanning aneugen and clastogen by micronuclei analysis using flow cytometry].
abstract: OBJECTIVE
  To explore a flow cytometry (FCM)-based method for discriminating aneugen- or clastogen-induced micronuclei.
  
  
  METHODS
  Cells were stained with anti-CD71-FITC and PI, and the PI fluorescent signal intensity of micronucleated reticulocyte (MN-RET) in the peripheral blood of NIH mouse treated with COL or CP was detected by flow cytometry.
  
  
  RESULTS
  The ratio of the median of the intensity of MN-RET fluorescent signals to that of nucleated cell was low in the cyclophosphamide treated mouse, while the median was high in the colchicine treated mouse.
  
  
  CONCLUSIONS
  The flow cytometry-based micronucleus assay can be used to discriminate primarily smaller MN induced by the clastogen exposure from the larger MN induced by an aneugen.
authors.affiliation: Guangdong Centre for Disease Control and Prerention, Guangzhou 510300, China.
authors.forename: Ming-Jie
authors.initials: MJ
authors.lastname: Yang
authors.forename: Jian-Chang
authors.initials: JC
authors.lastname: Zhou
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
authors.forename: Xing-Fen
authors.initials: XF
authors.lastname: Yang
authors.forename: Jun-Ming
authors.initials: JM
authors.lastname: Huang
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Tan
authors.forename: Jia
authors.initials: J
authors.lastname: Cao
authors.forename: Rui-Ping
authors.initials: RP
authors.lastname: Zeng
mesh_terms: D000818:Animals; D003078:Colchicine / Q000633:toxicity*; D003520:Cyclophosphamide / Q000633:toxicity*; D005434:Flow Cytometry / Q000379:methods; D008297:Male; D051379:Mice; D048629:Micronuclei, Chromosome-Defective; D009153:Mutagens / Q000633:toxicity; D012156:Reticulocytes / Q000187:drug effects* / Q000648:ultrastructure
chemical_list: D009153:Mutagens; D003520:Cyclophosphamide; D003078:Colchicine