A simple NMR analysis of the protonation equilibrium that accompanies aminoglycoside recognition: dramatic alterations in the neomycin-B protonation state upon binding to a 23-mer RNA aptamer.
A complete characterisation of the protonation equilibrium that accompanies the molecular recognition of neomycin-B by a specific RNA receptor has been achieved by employing simple NMR measurements.
Freire
Felix
F
Instituto de Química Orgánica General (CSIC), c/Juan de la Cierva 3, Madrid, Spain.
Cuesta
Igor
I
Corzana
Francisco
F
Revuelta
Julia
J
González
Carlos
C
Hricovini
Milos
M
Bastida
Agatha
A
Jiménez-Barbero
Jesús
J
Asensio
Juan Luis
JL
D000617:Aminoglycosides / Q000737:chemistry*; D000838:Anions / Q000737:chemistry; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001483:Base Sequence; D001665:Binding Sites; D005601:Framycetin / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D011522:Protons; D017186:Titrimetry
D000617:Aminoglycosides; D000838:Anions; D052157:Aptamers, Nucleotide; D011522:Protons; D005601:Framycetin