title: Regulation of developmental rate and germ cell proliferation in Caenorhabditis elegans by the p53 gene network.
abstract: Caenorhabditis elegans CEP-1 activates germline apoptosis in response to genotoxic stress, similar to its mammalian counterpart, tumor suppressor p53. In mammals, there are three p53 family members (p53, p63, and p73) that activate and repress many distinct and overlapping sets of genes, revealing a complex transcriptional regulatory network. Because CEP-1 is the sole p53 family member in C. elegans, analysis of this network is greatly simplified in this organism. We found that CEP-1 functions during normal development in the absence of stress to repress many (331) genes and activate only a few (28) genes. In response to genotoxic stress, 1394 genes are activated and 942 are repressed, many of which contain p53-binding sites. Comparison of the CEP-1 transcriptional network with transcriptional targets of the human p53 family reveals considerable overlap between CEP-1-regulated genes and homologues regulated by human p63 and p53, suggesting a composite p53/p63 action for CEP-1. We found that phg-1, the C. elegans Gas1 (growth arrest-specific 1) homologue, is activated by CEP-1 and is a negative regulator of cell proliferation in the germline in response to genotoxic stress. Further, we find that CEP-1 and PHG-1 mediate the decreased developmental rate and embryonic viability of mutations in the clk-2/TEL2 gene, which regulates lifespan and checkpoint responses.
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, CA 93106, USA. brent.derry@sickkids.ca
authors.forename: W B
authors.initials: WB
authors.lastname: Derry
authors.forename: R
authors.initials: R
authors.lastname: Bierings
authors.forename: M
authors.initials: M
authors.lastname: van Iersel
authors.forename: T
authors.initials: T
authors.lastname: Satkunendran
authors.forename: V
authors.initials: V
authors.lastname: Reinke
authors.forename: J H
authors.initials: JH
authors.lastname: Rothman
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000528:radiation effects; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000254:growth & development*; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism* / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D005786:Gene Expression Regulation / Q000528:radiation effects; D053263:Gene Regulatory Networks / Q000235:genetics / Q000528:radiation effects; D017238:Genes, Helminth / Q000235:genetics; D005854:Germ Cells / Q000254:growth & development* / Q000528:radiation effects; D006801:Humans; D034741:RNA, Small Interfering / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: C438244:CEP-1 protein, C elegans; D029742:Caenorhabditis elegans Proteins; D034741:RNA, Small Interfering; D016159:Tumor Suppressor Protein p53