Novel in vitro screening system for monoclonal antibodies using hypermutating chicken B cell library.
Here, we report an in vitro screening system for monoclonal antibodies using a hypermutating chicken B cell line, DT40-SW. When switching on hypermutation, cultured DT40-SW cells constituted an antibody library, from which clones secreting antibodies to a test antigen were successfully isolated, and genetically stabilized by switching off the mutation machinery.
Todo
Kagefumi
K
Department of Biotechnology, Graduate School of Natural Science and Technology, Okayama University, Tsushima-Naka, Okayama 700-8530, Japan.
Miyake
Kenji
K
Magari
Masaki
M
Kanayama
Naoki
N
Ohmori
Hitoshi
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001402:B-Lymphocytes / Q000378:metabolism*; D002460:Cell Line; D002645:Chickens; D003564:Cytidine Deaminase / Q000502:physiology; D009596:Nitrophenols / Q000276:immunology; D010648:Phenylacetates; D012710:Serum Albumin, Bovine / Q000276:immunology; D027041:Somatic Hypermutation, Immunoglobulin; D000098830:AICDA (Activation-Induced Cytidine Deaminase)
D000911:Antibodies, Monoclonal; D009596:Nitrophenols; D010648:Phenylacetates; C026242:4-hydroxy-5-nitrophenyl acetic acid; D012710:Serum Albumin, Bovine; D000098830:AICDA (Activation-Induced Cytidine Deaminase); D003564:Cytidine Deaminase