DNA-based molecular wires: multiple emission pathways of individual constructs.
The extent of photon energy transfer through individual DNA-based molecular wires composed of five dyes is investigated at the single molecular level. Combining single-molecule spectroscopy and pulse interleaved excitation imaging, we have directly resolved the time evolution spectral response of individual constructs, while simultaneously probing DNA integrity. Our data clearly show that intact wires exhibit photon-transfer efficiencies close to 100% across five dyes. Dynamical and multiple pathways for the photon emission resulting from conformational freedom of the wire are readily uncovered. These results provide the basis for guiding the synthesis of DNA-based supramolecular arrays with improved photon transport at the nanometer scale.
Sánchez-Mosteiro
Gabriel
G
Applied Optics Group, Faculty of Science and Technology and MESA+ Institute for Nanotechnology, University of Twente, P. O. Box 217, 7500 AE Enschede, The Netherlands.
van Dijk
Erik M H P
EM
Hernando
Jordi
J
Heilemann
Mike
M
Tinnefeld
Philip
P
Sauer
Markus
M
Koberlin
Felix
F
Patting
Matthias
M
Wahl
Michael
M
Erdmann
Rainer
R
van Hulst
Niek F
NF
García-Parajó
Maria F
MF
D004277:DNA, Single-Stranded / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D004277:DNA, Single-Stranded