Glutathione synthesis is regulated by nitric oxide in Medicago truncatula roots.
Glutathione (GSH) is one of the main antioxidants in plants. Legumes have the specificity to produce a GSH homolog, homoglutathione (hGSH). We have investigated the regulation of GSH and hGSH synthesis by nitric oxide (NO) in Medicago truncatula roots. Analysis of the expression level of gamma-glutamylcysteine synthetase (gamma-ECS), glutathione synthetase (GSHS) and homoglutathione synthetase (hGSHS) after treatment with sodium nitroprusside (SNP) and nitrosoglutathione (GSNO), two NO-donors, showed that gamma-ecs and gshs genes are up regulated by NO treatment whereas hgshs expression is not. Differential accumulation of GSH was correlated to gene expression in SNP-treated roots. Our results provide the first evidence that GSH synthesis pathway is regulated by NO in plants and that there is a differential regulation between gshs and hgshs in M. truncatula.
Innocenti
Gilles
G
Interactions Plantes-Microorganismes et Santé Végétale, UMR INRA 1064/Université de Nice-Sophia Antipolis/CNRS 6192, 400, route des Chappes, BP 167, 06903, Sophia-Antipolis, France.
Pucciariello
Chiara
C
Le Gleuher
Marie
M
Hopkins
Julie
J
de Stefano
Matteo
M
Delledonne
Massimo
M
Puppo
Alain
A
Baudouin
Emmanuel
E
Frendo
Pierre
P
D018506:Gene Expression Regulation, Plant; D005978:Glutathione / Q000096:biosynthesis*; D046913:Medicago truncatula / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism*
D010940:Plant Proteins; D009569:Nitric Oxide; D005978:Glutathione