Metronidazole-induced alterations in murine spermatozoa morphology.
The aim of this work was to assess the effect of metronidazole (MTZ) on the stages of the seminiferous epithelial cycle and spermatozoa morphology when the drug is administered in human therapeutic doses to 60-day-old CFW male mice. The frequency of the stages was established by counting spermatocytes in pachytene and spermatids. Abnormalities in the flagellum or the head, lack of maturity and multiple malformations, were considered in the morphological analysis. Murine control strain was compared with MTZ treated group (v.ip 130 mg/kg/bw) both kept in standard captivity conditions. Cellular composition or number of stages in the seminiferous tubules were not altered in MTZ exposed animals, though the number of cells in stages I, V and XII was increased. The sperm cell morphology was severely affected by the treatment with potentially serious consequences on the normal fertilization process. Thus, the MTZ has to be considered as a conceivable thread regarding male fertility.
Mudry
Marta D
MD
GIBE (Grupo de Investigación en Biología Evolutiva), Departamento Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA), Argentina.
Palermo
Ana M
AM
Merani
María S
MS
Carballo
Marta A
MA
D000818:Animals; D000981:Antiprotozoal Agents / Q000633:toxicity*; D002452:Cell Count; D005298:Fertility / Q000187:drug effects; D008297:Male; D008795:Metronidazole / Q000633:toxicity*; D051379:Mice; D008815:Mice, Inbred Strains; D049471:Pachytene Stage / Q000187:drug effects; D012671:Seminiferous Tubules / Q000187:drug effects / Q000473:pathology; D013087:Spermatids / Q000187:drug effects / Q000473:pathology; D013094:Spermatozoa / Q000187:drug effects* / Q000473:pathology
D000981:Antiprotozoal Agents; D008795:Metronidazole