title: Rapid quantification of bacterial cells in potable water using a simplified microfluidic device.
abstract: A simplified microfluidic device for quantification of bacteria in potable water was fabricated and examined. Comparisons of counts of Escherichia coli by the microfluidic system and by epifluorescence microscopy closely correlated (r2=0.99). Bacteria in natural mineral water and in purified household tap water were accurately enumerated by using this system within 15 min after fluorescent staining.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamada-oka, Suita, Osaka 565-0871, Japan.
authors.forename: Chieko
authors.initials: C
authors.lastname: Sakamoto
authors.forename: Nobuyasu
authors.initials: N
authors.lastname: Yamaguchi
authors.forename: Masumi
authors.initials: M
authors.lastname: Yamada
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Nagase
authors.forename: Minoru
authors.initials: M
authors.lastname: Seki
authors.forename: Masao
authors.initials: M
authors.lastname: Nasu
mesh_terms: D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000302:isolation & purification*; D005434:Flow Cytometry; D005618:Fresh Water / Q000382:microbiology*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods; D008856:Microscopy, Fluorescence; D008900:Mineral Waters / Q000382:microbiology*; D013997:Time Factors; D014881:Water Supply
chemical_list: D008900:Mineral Waters