[Differential proteomic expression in human liver cells stimulated by hydroquinone].
OBJECTIVE
To explore the differential proteomic expression in human liver cells L-02 after exposure to HQ.


METHODS
Subcultured L-02 cells were treated by HQ for 24 h at a 1 x 10(-4) mol/L concentration and a blank group was set as the control. Immediately after the treatment, total cellular proteins were extracted and separated by 2-DE, and the images were analyzed by PDQuest software. The experiment was totally repeated 3 times with 3 repetitions for each group every time. The well repeated spots were identified by MALDI-TOF MS and then searched in NCBI human protein database with Mascot.


RESULTS
About 1,000 spots per gel were found. Compared with the control group, 17, 18 and 24 spots were significantly altered in 3 separate experiments. The 4 well repeated spots were identified by MALDI-TOF MS as Rho GDP dissociation inhibitor GDI alpha, 6-phosphogluconolactonase, erbB3 binding protein EBP1 and lamin A/C, isoform 1 precursor. They were involved in cell skeleton, signal transduction and energy metabolization in functional classification.


CONCLUSIONS
Hydroquinone can change the protein expression in liver cells, which provides clues for exploring the toxic mechanism.
Ju
Li
L
Labour Health and Environmental Hygiene Institute, Zhejiang University, Hangzhou 310031, China.
Zhang
Shu-Zhi
SZ
Zhao
Ran
R
Yao
Geng-Dong
GD
D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D006873:Hydroquinones / Q000633:toxicity*; D013058:Mass Spectrometry; D040901:Proteomics; D015203:Reproducibility of Results
D006873:Hydroquinones; C031927:hydroquinone