title: Rapid and highly sensitive pathotyping of avian influenza A H5N1 virus by using real-time reverse transcription-PCR.
abstract: Rapid typing of the pathogenicity of avian influenza A viruses (AIV) of subtypes H5 and H7 is crucial to initiate adequate protective measures preventing the spread of highly pathogenic AIV (HPAIV). Here, a new real-time reverse transcription-PCR assay which enables sensitive and specific detection and cleavage site analysis of HPAIV H5N1 of the Qinghai lineage is described.
authors.affiliation: Institute of Diagnostic Virology, D-17493 Greifswald-Insel Riems, Germany.
authors.forename: Bernd
authors.initials: B
authors.lastname: Hoffmann
authors.forename: Timm
authors.initials: T
authors.lastname: Harder
authors.forename: Elke
authors.initials: E
authors.lastname: Starick
authors.forename: Klaus
authors.initials: K
authors.lastname: Depner
authors.forename: Ortrud
authors.initials: O
authors.lastname: Werner
authors.forename: Martin
authors.initials: M
authors.lastname: Beer
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000821:virology; D001483:Base Sequence; D001717:Birds / Q000821:virology; D002645:Chickens / Q000821:virology; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D053124:Influenza A Virus, H5N1 Subtype / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity*; D005585:Influenza in Birds / Q000453:epidemiology* / Q000517:prevention & control / Q000821:virology*; D008969:Molecular Sequence Data; D011201:Poultry Diseases / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors; D014422:Turkeys / Q000821:virology
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; C109963:hemagglutinin, avian influenza A virus