title: Coexpression and spatial association of nicotinic acetylcholine receptor subunits alpha7 and alpha10 in rat sympathetic neurons.
abstract: Fast excitatory synaptic transmission in sympathetic ganglia is mediated by nicotinic acetylcholine receptors (nAChRs). Although it is known that the nAChR alpha7-subunit occurs in sympathetic ganglia, the expression of the recently cloned subunit alpha10 (Elgoyhen et al., 2001; Lustig et al., 2001; Sgard et al., 2002) has not been analyzed. Until now, functional receptors containing alpha10-subunits have been found only in combination with alpha9-subunits (Elgoyhen et al., 2001; Lustig et al., 2001; Sgard et al., 2002). The alpha9-subunit exhibits a restricted expression pattern, whereas the alpha10-subunit is expressed more widely. This broad distribution resembles more closely that known for subunit alpha7 than for subunit alpha9. On this background, we investigated the distribution of nAChR subunits alpha7, alpha9, and alpha10 in rat sympathetic ganglia and studied a possible interaction between subunit alpha7 and potential partners by double-labeling immunofluorescence and fluorescence resonance energy transfer (FRET) (Kam et al., 1995; Jares-Erijman and Jovin, 2003).
authors.affiliation: Institute for Anatomy and Cell Biology and University of Giessen Lung Center, Justus-Liebig-University, 35385 Giessen, Germany. Katrin.S.Lips@anatomie.med.uni-giessen.de
authors.forename: Katrin Susanne
authors.initials: KS
authors.lastname: Lips
authors.forename: Peter
authors.initials: P
authors.lastname: König
authors.forename: Katrin
authors.initials: K
authors.lastname: Schätzle
authors.forename: Uwe
authors.initials: U
authors.lastname: Pfeil
authors.forename: Gabriela
authors.initials: G
authors.lastname: Krasteva
authors.forename: Markus
authors.initials: M
authors.lastname: Spies
authors.forename: Rainer Viktor
authors.initials: RV
authors.lastname: Haberberger
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Grando
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Kummer
mesh_terms: D000818:Animals; D005728:Ganglia, Sympathetic / Q000502:physiology*; D017403:In Situ Hybridization; D009474:Neurons / Q000502:physiology*; D021122:Protein Subunits / Q000737:chemistry* / Q000235:genetics*; D051381:Rats; D011978:Receptors, Nicotinic / Q000737:chemistry* / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D064569:alpha7 Nicotinic Acetylcholine Receptor
chemical_list: C470699:Chrna10 protein, rat; C579165:Chrna7 protein, rat; D021122:Protein Subunits; D011978:Receptors, Nicotinic; D064569:alpha7 Nicotinic Acetylcholine Receptor