[Composition diversity of lactic acid bacteria (LAB) community Al2 used for alfalfa silage].
Alfalfa is the most important forage grass that is difficult to ensile for good quality. Using silage inoculants are the important way for preservation of alfalfa silage. Through continuous restricted subcultivation, a lactic acid bacteria (LAB) community Al2 was selected from well-fermented alfalfa silage. Plate isolation and Denaturing Gradient Gel Electrophoresis (DGGE), construction of 16S rDNA clone library were used to identify the composition diversity of Al2 community, with 7 strains detected, and they were all belonged to Lactobacillus. The composition ratios of the 7 strains were 55.21%, 19.79%, 14.58%, 3.13%, 3.13%, 3.13%, 1.03% according to 16S rDNA clone library. Al2-1i, Al2-2i, Al2-3i, corresponding to L. plantarum (99.9%), L. kimchii (99.4%), L. farciminis (100%) were detected by plate isolation. Among 3 isolates, Al2-1i had the highest ability of dropping pH and producing lactic acid, and the amount of lactic acid was reach to 18g/L at 24h cultivated in MRS media. The ability of dropping pH and producing lactic acid of Al2-3i was the lowest. From DGGE profiles, the dominant strains in Al2 community were L. plantarum and L. kimchii. L. plantarum was detected during the whole process, and L. kimchii was detected in the later phase.
Wang
Xiao-Fen
XF
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China. wxiaofen_16@yahoo.com.cn
Gao
Li-Juan
LJ
Yang
Hong-Yan
HY
Wang
Wei-Dong
WD
Cui
Zong-Jun
ZJ
D004591:Electrophoresis, Polyacrylamide Gel; D005285:Fermentation; D007778:Lactobacillus / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D000455:Medicago sativa / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012820:Silage / Q000382:microbiology*
D012336:RNA, Ribosomal, 16S