Rapid and highly sensitive pathotyping of avian influenza A H5N1 virus by using real-time reverse transcription-PCR.
Rapid typing of the pathogenicity of avian influenza A viruses (AIV) of subtypes H5 and H7 is crucial to initiate adequate protective measures preventing the spread of highly pathogenic AIV (HPAIV). Here, a new real-time reverse transcription-PCR assay which enables sensitive and specific detection and cleavage site analysis of HPAIV H5N1 of the Qinghai lineage is described.
Hoffmann
Bernd
B
Institute of Diagnostic Virology, D-17493 Greifswald-Insel Riems, Germany.
Harder
Timm
T
Starick
Elke
E
Depner
Klaus
K
Werner
Ortrud
O
Beer
Martin
M
D000818:Animals; D000835:Animals, Wild / Q000821:virology; D001483:Base Sequence; D001717:Birds / Q000821:virology; D002645:Chickens / Q000821:virology; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D053124:Influenza A Virus, H5N1 Subtype / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity*; D005585:Influenza in Birds / Q000453:epidemiology* / Q000517:prevention & control / Q000821:virology*; D008969:Molecular Sequence Data; D011201:Poultry Diseases / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors; D014422:Turkeys / Q000821:virology
D019267:Hemagglutinin Glycoproteins, Influenza Virus; C109963:hemagglutinin, avian influenza A virus