title: Identification of genes that interact with Drosophila liquid facets.
abstract: We have performed mutagenesis screens of the Drosophila X chromosome and the autosomes for dominant enhancers of the rough eye resulting from overexpression of liquid facets. The liquid facets gene encodes the homolog of vertebrate endocytic Epsin, an endocytic adapter protein. In Drosophila, Liquid facets is a core component of the Notch signaling pathway required in the signaling cells for ligand endocytosis and signaling. Why ligand internalization by the signaling cells is essential for signaling is a mystery. The requirement for Liquid facets is a hint at the answer, and the genes identified in this screen provide further clues. Mutant alleles of clathrin heavy chain, Rala, split ends, and auxilin were identified as enhancers. We describe the mutant alleles and mutant phenotypes of Rala and aux. We discuss the relevance of all of these genetic interactions to the function of Liquid facets in Notch signaling.
authors.affiliation: Section of Molecular Cell and Developmental Biology, Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78712, USA.
authors.forename: Suk Ho
authors.initials: SH
authors.lastname: Eun
authors.forename: Kristi
authors.initials: K
authors.lastname: Lea
authors.forename: Erin
authors.initials: E
authors.lastname: Overstreet
authors.forename: Samuel
authors.initials: S
authors.lastname: Stevens
authors.forename: Ji-Hoon
authors.initials: JH
authors.lastname: Lee
authors.forename: Janice A
authors.initials: JA
authors.lastname: Fischer
mesh_terms: D000818:Animals; D034021:Auxilins / Q000235:genetics; D033922:Clathrin Heavy Chains / Q000235:genetics; D003001:Cloning, Molecular; D017931:DNA Primers; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004742:Enhancer Elements, Genetic / Q000235:genetics*; D017344:Genes, Insect / Q000235:genetics*; D005816:Genetic Complementation Test; D018398:Homeodomain Proteins / Q000235:genetics; D007150:Immunohistochemistry; D008855:Microscopy, Electron, Scanning; D016296:Mutagenesis; D009687:Nuclear Proteins / Q000235:genetics; D010641:Phenotype; D017956:Photoreceptor Cells, Invertebrate / Q000648:ultrastructure; D020161:Physical Chromosome Mapping; D016601:RNA-Binding Proteins; D017422:Sequence Analysis, DNA; D015398:Signal Transduction / Q000235:genetics*; D033921:Vesicular Transport Proteins / Q000378:metabolism*; D014921:Wings, Animal / Q000033:anatomy & histology; D014960:X Chromosome / Q000235:genetics*
chemical_list: D034021:Auxilins; D017931:DNA Primers; D029721:Drosophila Proteins; D018398:Homeodomain Proteins; C494336:Lqf protein, Drosophila; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; C402387:Spen protein, Drosophila; D033921:Vesicular Transport Proteins; D033922:Clathrin Heavy Chains