title: Regions in the transit peptide of SSU essential for transport into chloroplasts.
abstract: Deletion mutations, 3-19 amino acids in size, were introduced into the transit peptide (57 amino acids) of a small subunit (SSU) of ribulose-1,5-bisphosphate carboxylase/oxygenase from pea. Transport of the authentic small subunit precursor (pSSU) and of the mutant pSSUs by isolated chloroplasts of pea was examined. We show that the transit peptide contains two different, separated functional regions. A deletion mutation in the central region of the transit peptide, a region purported to be important for function, barely affected transport. Changes in the amino-terminal region of the transit peptide appeared normal. A deletion mutation at the carboxy-terminus of the transit peptide interfered with both transport and processing. From the aberrant processing we suggest that pSSU is matured in more than one step, and that the maturation signal is located within the carboxy-terminal 16 amino acids. The methionine residue at the evolutionarily conserved cleavage site (cysteine-methionine) between the transit peptide and the mature protein is not essential processing.
authors.affiliation: Department of Biochemistry, University of Arizona, Biological Sciences West, Tuscon, AZ 85721, USA.
authors.forename: B
authors.initials: B
authors.lastname: Reiss
authors.forename: C C
authors.initials: CC
authors.lastname: Wasmann
authors.forename: H J
authors.initials: HJ
authors.lastname: Bohnert
mesh_terms: D002736:Chloroplasts / Q000235:genetics*; D008565:Membrane Proteins / Q000378:metabolism; D009154:Mutation; D018532:Pisum sativum / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D021381:Protein Transport / Q000235:genetics; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D010940:Plant Proteins; D012273:Ribulose-Bisphosphate Carboxylase