title: Antioxidant activity of Albizzia lebbeck (Linn.) Benth. in alloxan diabetic rats.
abstract: There is an increasing demand for natural anti-diabetic drugs, as continuous oral administration of insulin can culminate in many side effects and toxicity. In our endeavour to formulate some cost-effective herbal medicines for diabetes, we undertook this study to evaluate the antioxidant potential of aqueous extract of Albizzia lebbeck (ALL) in diabetic rats. The oxidative stress in alloxan-induced diabetic rats was determined by estimating the levels of thiobarbituric acid reactive substances (TBARS), conjugated dienes (CD) and reduced glutathione (GSH) in liver and kidneys. Activities of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and glutathione S transferase (GST) were assessed in diabetic as well as rats co-administered with ALL. Oxidative damage in the liver and kidneys of diabetic rats as evidenced by a marked increment in the levels of TBARS and CD, and also a distinct diminution in GSH content was nullified by ALL, as these parameters showed a tendency to retrieve towards normalcy on co-administration of the herbal drug. The antioxidant enzymes registered a decline in activity in diabetic rats thus revealing the damaging effects of free radicals generated due to alloxan exposure. The activities of these enzymes returned to normalcy in ALL-administered rats indicating the antioxidant efficacy of the drug in resisting oxidative insult. The findings provide a rationale for further studies on isolation of active principles and pharmacological evaluation.
authors.affiliation: Pharmacognosy Research Laboratory, School of Biosciences, Mahatma Gandhi University, P.D. Hills P.O., Kottayam 686 560.
authors.forename: C R
authors.initials: CR
authors.lastname: Resmi
authors.forename: M R
authors.initials: MR
authors.lastname: Venukumar
authors.forename: M S
authors.initials: MS
authors.lastname: Latha
mesh_terms: D031289:Albizzia / Q000737:chemistry; D000496:Alloxan / Q000633:toxicity; D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology* / Q000627:therapeutic use; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy*; D004353:Drug Evaluation, Preclinical; D007328:Insulin / Q000009:adverse effects / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy / Q000379:methods; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D007328:Insulin; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; D000496:Alloxan