Effects of photodynamic therapy using 5-aminolevulinic acid on cultured human adenomyosis-derived cells.
OBJECTIVE
To evaluate the potential of photodynamic therapy with aminolevulinic acid (ALA) on human adenomyosis-derived cells compared with endometrial stromal cells.


METHODS
In vitro study.


METHODS
Department of Obstetrics and Gynecology, Tohoku University Hospital.


METHODS
Women with adenomyosis attending the University hospital.


METHODS
Photodynamic treatment.


METHODS
Cell survival rates.


RESULTS
Treatment with both ALA and irradiation caused significantly decreased survival of cells derived from human adenomyosis compared with ALA or irradiation alone. The combination of irradiation and ALA led to 79.3%, 68.0%, and 59.5% cell survival at 1.6, 4, and 8 J/cm2, respectively, whereas ALA and irradiation alone caused 92.8% and 97% survival, respectively.


CONCLUSIONS
Photodynamic therapy using ALA caused extensive death of cells derived from human adenomyosis. Photodynamic treatment using ALA may be a new treatment for patients with adenomyosis uteri in the future.
Suzuki-Kakisaka
Haruka
H
Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Miyagi, Japan. suharuka@mail.tains.tohoku.ac.jp
Murakami
Takashi
T
Hirano
Toru
T
Terada
Yukihiro
Y
Yaegashi
Nobuo
N
Okamura
Kunihiro
K
D000328:Adult; D000622:Aminolevulinic Acid / Q000008:administration & dosage*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D004715:Endometriosis / Q000188:drug therapy* / Q000473:pathology*; D004717:Endometrium / Q000187:drug effects* / Q000473:pathology*; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D008875:Middle Aged; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000008:administration & dosage; D016896:Treatment Outcome
D017319:Photosensitizing Agents; D000622:Aminolevulinic Acid