Microdevice-based delivery of gene products using sonoporation.
This paper presents a proof-of-concept miniature device for delivery of antisense oligonucleotides (ASO). A piezoelectric, lead zirconate titanate (PZT) plate (0.5 cm(2) x 0.75 mm) is used to transfect cells using cavitation-induced sonoporation. Both human umbilical vein endothelial cells (HUVEC) and human prostate cancer cells (PC3) are investigated in vitro. Preliminary results show that after sonication, the transfection rate for HUVEC increases by 96% compared to controls (p < 0.01). For PC3, the transfection rate increases by 31% compared to controls (p < 0.02). This research can potentially be applied in realizing a microelectromechanical system (MEMS)-based device for gene therapy in cancer treatment.
Siu
Tung
T
Department of Mechanical Engineering, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.
Rohling
Robert
R
Chiao
Mu
M
D045744:Cell Line, Tumor; D004247:DNA / Q000008:administration & dosage*; D018274:Electroporation / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D015316:Genetic Therapy / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D008297:Male; D008904:Miniaturization; D011471:Prostatic Neoplasms / Q000235:genetics / Q000628:therapy*; D013010:Sonication / Q000295:instrumentation*; D014162:Transfection / Q000295:instrumentation* / Q000379:methods; D051020:bcl-X Protein / Q000008:administration & dosage* / Q000235:genetics
C494811:BCL2L1 protein, human; D051020:bcl-X Protein; D004247:DNA