Purification of PP2Ac from bovine heart.
The catalytic subunit of PP2A (PP2Ac) can be purified in milligram quantities from bovine heart using ethanol precipitation, ammonium sulfate precipitation, ion exchange and size exclusion chromatography. The detailed procedure is described to purify PP2Ac over 4 d.
Tran
Hue T
HT
Department of Biological Sciences, University of Calgary, Alberta, Canada.
Ferrar
Tony S
TS
Ulke-Lemée
Anne
A
Moorhead
Greg B G
GB
D000645:Ammonium Sulfate / Q000737:chemistry; D000818:Animals; D020134:Catalytic Domain; D002417:Cattle; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D000431:Ethanol / Q000737:chemistry; D009206:Myocardium / Q000201:enzymology*; D010749:Phosphoprotein Phosphatases / Q000737:chemistry / Q000302:isolation & purification*
D000431:Ethanol; D010749:Phosphoprotein Phosphatases; D000645:Ammonium Sulfate