DNA damage and repair process in earthworm after in-vivo and in vitro exposure to soils irrigated by wastewaters.
In this study, DNA damage to earthworms (Eisenia fetida) after in vivo exposure to contaminated soils was measured by detecting DNA strand breakages (DSBs) and causality was analyzed through fractionation based bioassays. A non-linear dose-response relationship existed between DNA damage and total soil PAHs levels. DNA damage, measured with the comet assay, and its repair process, were observed. To identify the chemical causality, an in vitro comet assay using coelomocytes was subsequently performed on the fractionated organic extracts from soils. The results showed that the PAHs in the soils were responsible for the exerting genotoxic effects on earthworms. When normalized to benzo(a)pyrene toxic equivalent (TEQ(BaP)), the saturation dose in the dose-response curve was about 10ng TEQ(BaP) g(-1) soil (dw).
Qiao
Min
M
State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China.
Chen
Ying
Y
Wang
Chun-Xia
CX
Wang
Zijian
Z
Zhu
Yong-Guan
YG
D000818:Animals; D001681:Biological Assay; D020552:Comet Assay; D004249:DNA Damage; D004260:DNA Repair; D004787:Environmental Pollution / Q000009:adverse effects*; D009835:Oligochaeta / Q000235:genetics* / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*; D012989:Soil Pollutants / Q000633:toxicity*; D014865:Waste Disposal, Fluid / Q000379:methods*
D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants