Rescue of chimeric adenoviral vectors to expand the serotype repertoire.
The successful use of any adenoviral vectors is predicated upon the use of a serotype that is not neutralized by circulating antibodies. However, efforts to develop a diverse repertoire of serologically distinct adenovirus vectors may be hindered by the necessity to generate cell lines to allow for the successful propagation of vectors deleted of essential genes. A strategy to construct chimeric adenoviruses whereby the rescue and propagation of an E1-deleted HAdV-B-derived adenoviral vector can be achieved using existing cell lines such as HEK 293 is reported. It is further shown that this strategy may be more widely applicable.
Roy
Soumitra
S
Department of Pathology and Laboratory Medicine, Division of Transfusion Medicine, Gene Therapy Program, Translational Research Laboratory, 125 South 31st Street, Suite 2000, Philadelphia, PA 19104-3403, USA.
Clawson
David S
DS
Lavrukhin
Oleg
O
Sandhu
Arbans
A
Miller
Jim
J
Wilson
James M
JM
D017498:Adenovirus E1 Proteins / Q000235:genetics / Q000276:immunology; D017504:Adenovirus E4 Proteins / Q000235:genetics / Q000276:immunology; D000261:Adenoviruses, Simian / Q000145:classification / Q000235:genetics* / Q000276:immunology; D000818:Animals; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics*; D005240:Feasibility Studies; D005822:Genetic Vectors; D016679:Genome, Viral; D006801:Humans; D008957:Models, Genetic; D009500:Neutralization Tests; D002679:Pan troglodytes / Q000821:virology; D010957:Plasmids; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D015183:Restriction Mapping; D012703:Serotyping; D014162:Transfection
D017498:Adenovirus E1 Proteins; D017504:Adenovirus E4 Proteins; D004279:DNA, Viral; D011994:Recombinant Proteins