title: Real-time monitoring for bioaerosols--flow cytometry.
abstract: Bioaerosol detection in real time is an urgent civilian and military requirement. In this article, bioaerosol mass spectrometry, an instrument for real-time detection of bioaerosols using simultaneous measurement of particle aerodynamic size and intrinsic fluorescence, real-time qPCR, and FCM/FL were discussed. Although, challenging work remains to determine the interfering substances (e.g. particulates) of different environments, distinguish the specific species with specific probe, and overcome the high detection limit of FCM (10(4)-10(8) cells ml(-1)), literature reports suggested that FCM/FL has a great potential for real-time monitoring of bioaerosols.
authors.affiliation: Department of Public Health, Kaohsiung Medical University, Kaohsiung, Taiwan.
authors.forename: Pei-Shih
authors.initials: PS
authors.lastname: Chen
authors.forename: Chih-Shan
authors.initials: CS
authors.lastname: Li
mesh_terms: D000336:Aerosols; D000391:Air Microbiology; D000397:Air Pollution / Q000032:analysis*; D001694:Biological Warfare; D005434:Flow Cytometry; D013058:Mass Spectrometry; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000336:Aerosols