title: Immunochromatography using colloidal gold-antibody probe for the detection of atrazine in water samples.
abstract: An immunochromatography (ICG) strip test for rapid detection of atrazine in water samples was developed. A monoclonal antibody (MAb) specific to atrazine was produced from the cloned hybridoma cell (AT-1-M3) and used to develop a direct competitive enzyme-linked immunosorbent assay (DC-ELISA) and ICG strip. MAb conjugated to colloidal gold, and that was applied to the conjugate pad of the ICG strip. The visual detection limit for the ICG strip was 3 ng/mL. This test required only 10 min to get results and one step of sample to perform the assay. The results of water samples spiked with 5, 10, 20, and 50 ng/mL of atrazine by ICG strip were in good agreement with those obtained by DC-ELISA. The ICG strip was sufficiently sensitive and accurate to be useful for rapid screening of atrazine in various water samples.
authors.affiliation: Division of Applied Life Science (Brain Korea 21 program), Graduate School of Gyeongsang National University, Chinju, Gyeongnam 660-701, Korea.
authors.forename: Won-Bo
authors.initials: WB
authors.lastname: Shim
authors.forename: Zheng-You
authors.initials: ZY
authors.lastname: Yang
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Jin-Gil
authors.initials: JG
authors.lastname: Choi
authors.forename: Jung-Hyun
authors.initials: JH
authors.lastname: Je
authors.forename: Sung-Jo
authors.initials: SJ
authors.lastname: Kang
authors.forename: Anna Yu
authors.initials: AY
authors.lastname: Kolosova
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Eremin
authors.forename: Duck-Hwa
authors.initials: DH
authors.lastname: Chung
mesh_terms: D000911:Antibodies, Monoclonal; D001280:Atrazine / Q000032:analysis*; D002845:Chromatography / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D017979:Gold Colloid; D006540:Herbicides / Q000032:analysis*; D007118:Immunoassay / Q000379:methods*; D014867:Water / Q000737:chemistry*
chemical_list: D000911:Antibodies, Monoclonal; D017979:Gold Colloid; D006540:Herbicides; D014867:Water; D001280:Atrazine