Lasting neuroendocrine-immune effects of traumatic brain injury in rats.
Traumatic brain injury (TBI) is a principal cause of long-term physical, cognitive, behavioral, and social deficits in young adults, which frequently coexist with a high incidence of substance abuse disorders. However, few studies have examined the long-term effects of TBI on the neuroendocrine-immune system. TBI was induced in adult male rats under isoflurane anesthesia by cortical contusion injury with a pneumatic piston positioned stereotaxically over the left parietal cortex. Controls underwent sham surgery without injury. At 4 weeks post-injury, the plasma corticosterone response to 30-min restraint stress was significantly blunted in TBI rats compared to the sham controls. One week later, transmitters were implanted for continuous biotelemetric recording of body temperature and spontaneous locomotor activity. At 6 weeks post-injury, the febrile response to i.p. injection of the bacterial endotoxin, lipopolysaccharide (LPS; 50 microg/kg), was significantly lower in TBI than in sham rats. At 8 weeks, swimming in the forced swim test was significantly less in TBI than sham rats. At 9 weeks, rats were rendered ethanol (EtOH) dependent by feeding an EtOH-containing liquid diet for 14 days. Cosine rhythmometry analysis of circadian body temperature Midline Estimating Statistic of Rhythm (MESOR), amplitudes, and acrophases indicated differential effects of EtOH and withdrawal in the two groups. Light- and dark-phase activity analysis indicated that TBI rats were significantly more active than the sham group, and that EtOH and withdrawal differentially affected their activity. Given the extensive interactions of the neuroendocrine-immune systems, these results demonstrate that TBI produces lasting dysregulation amidst the central substrates for allostasis and circadian rhythmicity.
Taylor
Anna N
AN
Department of Neurobiology, Brain Research Institute and Brain Injury Research Center, David Geffen School of Medicine at UCLA, West Los Angeles Healthcare Center, Los Angeles, California 90095-1763, USA. ataylor@mednet.ucla.edu
Rahman
Shayan U
SU
Tio
Delia L
DL
Sanders
Matthew J
MJ
Bando
Jennifer K
JK
Truong
Amy H
AH
Prolo
Paolo
P
D019973:Alcohol-Related Disorders / Q000209:etiology; D052636:Allostasis / Q000502:physiology*; D000818:Animals; D001833:Body Temperature Regulation / Q000502:physiology; D001930:Brain Injuries / Q000097:blood / Q000503:physiopathology* / Q000523:psychology; D002940:Circadian Rhythm / Q000502:physiology; D003345:Corticosterone / Q000097:blood; D007107:Immune System / Q000503:physiopathology*; D008297:Male; D009043:Motor Activity / Q000502:physiology; D009490:Neurosecretory Systems / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D003345:Corticosterone