GAS6 is an estrogen-inducible gene in mammary epithelial cells.
To identify estrogen-responsive genes in mammary glands, microarray assays were performed. Twenty genes were found to be up-regulated while 16 genes were repressed in the 9h estrogen treated glands. The induction of GAS6, one of the genes up-regulated by estrogen, was confirmed by RNase protection assay. Furthermore, GAS6 was also demonstrated to be induced by estrogen in ER positive breast cancer cells. Analysis of GAS6 promoter revealed that GAS6 promoter was regulated by estrogen. An estrogen response element (ERE) was identified in the GAS6 promoter. Electrophoretic mobility shift assay revealed that ERalpha interacted with the ERE in the GAS6 promoter. Chromatin immunoprecipitation demonstrated that ERalpha was recruited to the GAS6 promoter upon estrogen stimulation. These results suggested that GAS6 is an estrogen target gene in mammary epithelial cells.
Mo
Rigen
R
Department of Pathology, Feinberg School of Medicine, Northwestern University, 303 East Chicago Avenue, Chicago, IL 60611-3008, USA.
Tony Zhu
Yiwei
Y
Zhang
Zhongyi
Z
Rao
Sambasiva M
SM
Zhu
Yi-Jun
YJ
D000818:Animals; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D004967:Estrogens / Q000494:pharmacology*; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D011960:Receptors, Estrogen / Q000378:metabolism*; D000099255:Growth Arrest-Specific Protein 6
D004967:Estrogens; D036341:Intercellular Signaling Peptides and Proteins; D011960:Receptors, Estrogen; D000099255:Growth Arrest-Specific Protein 6