title: Equal performance of TaqMan, MGB, molecular beacon, and SYBR green-based detection assays in detection and quantification of roundup ready soybean.
abstract: We have tested and compared the performance of 12 different assays representing four different real-time polymerase chain reaction (PCR) chemistries in the context of genetically modified organism detection. Several different molecular beacon, SYBR Green, TaqMan, and MGB assays were designed for the event specific detection and quantification of the 3' integration junction of GTS 40-3-2 (Roundup Ready) soybean. Sensitivity as well as robustness in the presence of background DNA were tested. None of the PCR-based approaches appeared to be significantly better than any of the other, but the molecular beacon assays had the lowest efficiency and also seemed more sensitive to changes in experimental setup.
authors.affiliation: Section of Food and Feed Microbiology, National Veterinary Institute, Ullevaalsveien 68, 0454 Oslo, Norway.
authors.forename: Charlotte Bøydler
authors.initials: CB
authors.lastname: Andersen
authors.forename: Arne
authors.initials: A
authors.lastname: Holst-Jensen
authors.forename: Knut Gunnar
authors.initials: KG
authors.lastname: Berdal
authors.forename: Tage
authors.initials: T
authors.lastname: Thorstensen
authors.forename: Torstein
authors.initials: T
authors.lastname: Tengs
mesh_terms: D018744:DNA, Plant / Q000032:analysis*; D005456:Fluorescent Dyes; D030821:Plants, Genetically Modified / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013025:Glycine max / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D005456:Fluorescent Dyes