Stress-induced facilitation of the cortisol response in 17alpha-hydroxylase deficient XX mas-1/mas-1 carp (Cyprinus carpio).
Facilitation of the stress response results from a reduction of the inhibitory effects of circulating corticosteroids, allowing an animal to respond to a novel stressor. In this study, the existence of a facilitated cortisol stress response in normal (STD) and 17alpha-hydroxylase deficient XX mas-1/mas-1 (E5) carp was investigated. E5 carp had previously been characterized as having a low cortisol response to stress. Fish were subjected to either cortisol feeding or daily-acute stress, from 45 until 140 days post-hatch (dph) and were then subjected to a novel net-confinement stressor at 141 dph. Growth of E5 fish was reduced in both the daily-acute stress and cortisol-fed groups, but STD fish were only affected by daily-acute stress. Cortisol feeding had no effect on the stress response of STD fish but daily-acute stress significantly inhibited the response to a subsequent novel stressor. In contrast, daily-acute stress facilitated the cortisol stress response of E5 fish to a novel stressor, while cortisol feeding inhibited the cortisol response. Facilitation was accompanied by significant enlargement of the head-kidney tissue (which contains the steroidogenic interrenal tissue) in E5 fish. To our knowledge this is the first report of stress-induced facilitation in a lower vertebrate.
Ruane
N M
NM
Wageningen Institute of Animal Sciences (WIAS), Wageningen University, PO Box 338, 6700 AH Wageningen, The Netherlands. Neil.Ruane@marine.ie
Th Goos
H J
HJ
Komen
J
J
D000818:Animals; D030801:Animals, Genetically Modified; D002347:Carps / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D005260:Female; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D006854:Hydrocortisone / Q000097:blood*; D007401:Interrenal Gland / Q000201:enzymology*; D008297:Male; D013254:Steroid 17-alpha-Hydroxylase / Q000235:genetics / Q000378:metabolism*; D013315:Stress, Psychological / Q000201:enzymology* / Q000378:metabolism
D029941:Fish Proteins; D013254:Steroid 17-alpha-Hydroxylase; D006854:Hydrocortisone