Optical imaging of bacterial infection in living mice using a fluorescent near-infrared molecular probe.
An optical imaging probe was synthesized by attaching a near-infrared carbocyanine fluorophore to an affinity group containing two zinc(II) dipicolylamine (Zn-DPA) units. The probe has a strong and selective affinity for the surfaces of bacteria, and it was used to image infections of Gram-positive S. aureus and Gram-negative E. coli bacteria in living nude mice. After intravenous injection, the probe selectively accumulates at the sites of localized bacterial infections in the thigh muscles of the mice.
Leevy
W Matthew
WM
Department of Chemistry and Biochemistry, 251 Nieuwland Science Hall, University of Notre Dame, Notre Dame, IN 46556, USA.
Gammon
Seth T
ST
Jiang
Hua
H
Johnson
James R
JR
Maxwell
Dustin J
DJ
Jackson
Erin N
EN
Marquez
Manuel
M
Piwnica-Worms
David
D
Smith
Bradley D
BD
D000818:Animals; D004195:Disease Models, Animal; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D051379:Mice; D015335:Molecular Probes / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D010847:Picolines / Q000138:chemical synthesis / Q000737:chemistry*; D012680:Sensitivity and Specificity; D019265:Spectroscopy, Near-Infrared / Q000379:methods; D013203:Staphylococcal Infections / Q000175:diagnosis*; D013211:Staphylococcus aureus / Q000737:chemistry; D041622:Tomography, Optical / Q000379:methods*
D005456:Fluorescent Dyes; D015335:Molecular Probes; D009942:Organometallic Compounds; D010847:Picolines; C509592:zinc(II) dipicolylamine