[Molecular typing of the pathogenic Yersinia enterocolitica strains with pulsed field gel electrophores isolated in China].
OBJECTIVE
To investigate the epidemiological and molecular typing features of the pathogenic Yersinia enterocolitica strains isolated in China,using pulsed field gel electrophoresis(PFGE) and standardized PFGE method as well as typing database of Yersinia enterocolitica.


METHODS
PFGE analysis was performed as Laboratory Directions for molecular subtyping of Salmonella by PFGE (PulseNet,USA) with some modifications and the results of PFGE were analyzed by BioNumerics soft (Version 4.0, Applied Maths BVBA, Belium).


RESULTS
114 O:3 Yersinia enterocolitica strains were typed by 25 patterns to have found that K6GN11C30012 (50 strains), K6GN11C30015(19 strains) and K6GN11C30016(10 strains) were the major patterns. K6GNllC30012 had 92.2% cluster similarity with K6GN11C30009-K6GN11C30023. This clone included 91.23% strains of 114 0:3 Yersinia enterocolitica strains. 51 0:9 Yersinia enterocolitica strains were typed by 14 patterns; K6GN11C90004 (22 strains) and K6GN11C90010 (13 strains)were the major patterns. K6GN11C90004 had 81.8% cluster similarity with K6GN11C90010 patterns. The major patterns of 0:3 and 0:9 serotypes were quite different.


CONCLUSIONS
O:3 Yersinia enterocolitica strains might originate from the same clone and had very few variation in different years and provinces but O:9 Yersinia enterocolitica strains from two different clones with some changes.
Jin
Dong
D
National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Cui
Zhi-gang
ZG
Xiao
Yu-chun
YC
Wang
Xin
X
Gu
Feng
F
Xia
Sheng-li
SL
Hu
Wan-fu
WF
Yang
Jin-chuan
JC
Wang
Hua
H
Gu
Ling
L
Xu
Jian-Guo
JG
Kan
Biao
B
Jing
Huai-qi
HQ
D002681:China; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D015008:Yersinia enterocolitica / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification