title: Laparoscopic ovariectomy for whole human ovary cryopreservation: technical aspects.
abstract: OBJECTIVE
  To describe the technique of laparoscopic ovariectomy with a view to cryopreservation of a whole ovary with its vascular pedicle.
  
  
  METHODS
  Descriptive study.
  
  
  METHODS
  Gynecology research unit in a department of gynecology in a university hospital.
  
  
  METHODS
  Women with indications for chemotherapy or radiotherapy who are at high risk of premature ovarian failure.
  
  
  METHODS
  Laparoscopic ovariectomy for whole ovary cryopreservation in nine patients.
  
  
  METHODS
  Feasibility of laparoscopic ovariectomy for whole ovary cryopreservation and later autotransplantation without delaying chemotherapy.
  
  
  RESULTS
  The whole ovary was successfully removed by laparoscopy and cryopreserved by arterial catheterization in all nine patients.
  
  
  CONCLUSIONS
  Ovariectomy with a view to whole ovary cryopreservation for future transplantation may be performed by laparoscopy. Great care must be taken to remove the ovary, together with a large part (> or =5 cm) of the infundibulopelvic ligament, allowing dissection of the ovarian vessels, perfusion with a cryoprotective medium, and cryopreservation for subsequent autografting of the whole ovary. The period of ischemia between ligation of the ovarian pedicle and ovarian cryopreservation must be as short as possible.
authors.affiliation: Department of Gynecology, Université Catholique de Louvain, Cliniques Universitaires Saint-Luc, Brussels, Belgium.
authors.forename: Pascale
authors.initials: P
authors.lastname: Jadoul
authors.forename: Jacques
authors.initials: J
authors.lastname: Donnez
authors.forename: Marie-Madeleine
authors.initials: MM
authors.lastname: Dolmans
authors.forename: Jean
authors.initials: J
authors.lastname: Squifflet
authors.forename: Benoît
authors.initials: B
authors.lastname: Lengele
authors.forename: Belen
authors.initials: B
authors.lastname: Martinez-Madrid
mesh_terms: D015925:Cryopreservation; D005260:Female; D006801:Humans; D010535:Laparoscopy / Q000379:methods*; D010052:Ovariectomy / Q000379:methods*; D010053:Ovary / Q000098:blood supply / Q000637:transplantation*; D016649:Primary Ovarian Insufficiency / Q000601:surgery*