title: Purification of PP2Ac from bovine heart.
abstract: The catalytic subunit of PP2A (PP2Ac) can be purified in milligram quantities from bovine heart using ethanol precipitation, ammonium sulfate precipitation, ion exchange and size exclusion chromatography. The detailed procedure is described to purify PP2Ac over 4 d.
authors.affiliation: Department of Biological Sciences, University of Calgary, Alberta, Canada.
authors.forename: Hue T
authors.initials: HT
authors.lastname: Tran
authors.forename: Tony S
authors.initials: TS
authors.lastname: Ferrar
authors.forename: Anne
authors.initials: A
authors.lastname: Ulke-Lemée
authors.forename: Greg B G
authors.initials: GB
authors.lastname: Moorhead
mesh_terms: D000645:Ammonium Sulfate / Q000737:chemistry; D000818:Animals; D020134:Catalytic Domain; D002417:Cattle; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D000431:Ethanol / Q000737:chemistry; D009206:Myocardium / Q000201:enzymology*; D010749:Phosphoprotein Phosphatases / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D000431:Ethanol; D010749:Phosphoprotein Phosphatases; D000645:Ammonium Sulfate