In vitro and in vivo effects of hydrolysates from conglycinin on intestinal microbial community of mice after Escherichia coli infection.
OBJECTIVE
To detect the effect of pepsin-hydrolysate conglycinin (PTC) on the growth of Escherichia coli O(138)in vitro, and investigate the effect of PTC on intestinal microbial community of mice after E. coli infection.


RESULTS
Serial dilution method was used to detect the antibacterial activity of PTC in 96-well cell-cultivated plates. Fifty-five KM mice were randomly assigned to five groups: normal, feeding-E. coli control, HCl-full hydrolysis of conglycinin, conglycinin and PTC. Orally administrated with hydrolysates from conglycinin for 21 days, each mouse was fed with 2 x 10(8) CFU ml(-1) of E. coli O(138) on the 22nd day. The mice activities were monitored and polymerase chain reaction-denaturing gradient gel electrophoresis was used to analyse the microbial community in mice faeces. The results showed that PTC could inhibit growth of E. coli O(138) at nitrogen concentrations of more than 520 mg l(-1). There was high similarity of intestinal microbial community in mice between PTC and normal groups.


CONCLUSIONS
PTC inhibits growth of E. coli O(138), keeps mice healthy following oral administration of E. coli infection and maintains a balanced active microbial community in their gastrointestinal tract.


CONCLUSIONS
This study demonstrated the antibacterial activity of PTC against E. coli and its ability to maintain healthy intestinal microbial community in mice even after they were infected with E. coli. This observation is significant in the application of PTC to prevent gastrointestinal diseases caused by E. coli and unbalanced intestinal microflora.
Shen
C-L
CL
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province, China.
Chen
W-H
WH
Zou
S-X
SX
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D052179:Antigens, Plant; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development*; D004927:Escherichia coli Infections / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D005916:Globulins / Q000494:pharmacology*; D006868:Hydrolysis; D007422:Intestines / Q000382:microbiology; D008297:Male; D051379:Mice; D010434:Pepsin A / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D055314:Seed Storage Proteins; D030262:Soybean Proteins / Q000494:pharmacology*; D013025:Glycine max / Q000737:chemistry
D000900:Anti-Bacterial Agents; D052179:Antigens, Plant; D005916:Globulins; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C100565:alpha-conglycinin, Glycine max; C013894:beta-conglycinin protein, Glycine max; D010434:Pepsin A