title: Effects of PM2.5 components in the release of amphiregulin by human airway epithelial cells.
abstract: Exposure to ambient particulate matter (PM) is responsible for airway inflammation and tissue remodeling. Urban PM(2.5) (aerodynamic diameter <2.5microm) is a complex mixture rich in soots and containing hydrosoluble and organic components. We previously showed that the exposure of airway epithelial cells to PM(2.5) triggers the release of amphiregulin (AR), ligand of the epidermal growth factor receptor (EGFR) involved in proinflammatory and repair responses. The effect of Paris PM(2.5) organic and aqueous fractions in AR expression and secretion was investigated on the bronchial epithelial cell line 16HBE and normal human nasal epithelial (NHNE) cells. Both a macroarray specific for inflammation pathways and RT-PCR showed an AR upregulation in organic extract-treated 16HBE cells. AR release is induced in 16HBE and NHNE cells grown on plastic and exposed to native PM(2.5), organic extract and to a lesser extent washed PM(2.5) (deprived of its hydrosoluble content) and aqueous extract. Furthermore, as assessed by using NHNE cells grown on Transwell inserts, this secretion is polarized toward the basolateral side where the EGFR is expressed. To conclude, both PM(2.5) organic and hydrosoluble components are involved in the expression and secretion of AR; organic compounds exhibiting a strong effect when they are easily bioavailable.
authors.affiliation: Laboratoire de Cytophysiologie et de Toxicologie Cellulaire, Tour 53-54, 3(e) étage, Case Courrier 7073, Université Paris 7, 2 Place Jussieu, 75251 Paris Cedex 05, France. melina.rumelhard@paris7.jussieu.fr
authors.forename: Mélina
authors.initials: M
authors.lastname: Rumelhard
authors.forename: Kiran
authors.initials: K
authors.lastname: Ramgolam
authors.forename: Floriane
authors.initials: F
authors.lastname: Auger
authors.forename: Anne-Catherine
authors.initials: AC
authors.lastname: Dazy
authors.forename: Sophie
authors.initials: S
authors.lastname: Blanchet
authors.forename: Francelyne
authors.initials: F
authors.lastname: Marano
authors.forename: Armelle
authors.initials: A
authors.lastname: Baeza-Squiban
mesh_terms: D000393:Air Pollutants / Q000032:analysis / Q000633:toxicity*; D066258:Amphiregulin; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry; D066255:EGF Family of Proteins; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D018773:Genes, erbB-1 / Q000235:genetics; D006023:Glycoproteins / Q000096:biosynthesis / Q000378:metabolism*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis / Q000378:metabolism*; D008670:Metals / Q000032:analysis / Q000633:toxicity; D018613:Microscopy, Confocal; D009297:Nasal Mucosa / Q000166:cytology / Q000187:drug effects; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000633:toxicity; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014420:Turbinates / Q000166:cytology / Q000187:drug effects
chemical_list: C587848:AREG protein, human; D000393:Air Pollutants; D066258:Amphiregulin; D066255:EGF Family of Proteins; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; D008670:Metals; D011084:Polycyclic Aromatic Hydrocarbons; D012333:RNA, Messenger