title: Cell cycling and differentiation do not require the retinoblastoma protein during early Xenopus development.
abstract: The retinoblastoma protein (pRb) is a central regulator of the cell cycle, controlling passage through G1 phase. Moreover, pRb has also been shown to play a direct role in the differentiation of multiple tissues, including nerve and muscle. Rb null mice display embryonic lethality, although recent data have indicated that at least some of these defects are due to placental insufficiency. To investigate this further, we have examined the role of pRb in early development of the frog Xenopus laevis, which develops without the need for a placenta. Surprisingly, we see that loss of pXRb has no effect on either cell cycling or differentiation of neural or muscle tissue, while overexpression of pXRb similarly has no effects. We demonstrate that, in fact, pXRb is maintained in a hyperphosphorylated and therefore inactive state early in development. Therefore, Rb protein is not required for cell cycle control or differentiation in early embryos, indicating unusual control of these G1/G0 events at this developmental stage.
authors.affiliation: Department of Oncology, University of Cambridge, Hutchison/MRC Research Centre, Addenbrookes Hospital, Hills Road, Cambridge CB2 0XZ, UK.
authors.forename: Ruth A
authors.initials: RA
authors.lastname: Cosgrove
authors.forename: A
authors.initials: A
authors.lastname: Philpott
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001973:Bromodeoxyuridine; D002453:Cell Cycle / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology*; D047108:Embryonic Development / Q000502:physiology*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D020287:In Situ Nick-End Labeling; D009841:Oligonucleotides; D016160:Retinoblastoma Protein / Q000378:metabolism*; D014982:Xenopus laevis / Q000196:embryology*
chemical_list: D009841:Oligonucleotides; D016160:Retinoblastoma Protein; D001973:Bromodeoxyuridine