The protein encoded by the gene proliferation disrupter (prod) is associated with the telomeric retrotransposon array in Drosophila melanogaster.
We report in this paper that the PROD protein, encoded by the gene proliferation disrupter (prod), is associated with the telomeric chromatin in Drosophila melanogaster. It binds to a region just upstream of the promoter of the telomere-specific retrotransposon HeT-A, which is located in the long 3'untranslated region of the element near its oligo(A) tail. Reduction of PROD in prod heterozygote flies results in elevated levels of HeT-A RNA in the ovaries, suggesting that PROD functions as a repressor of HeT-A transcriptional activity at the telomeres.
Török
Tibor
T
Department of Genetics and Molecular Biology, University of Szeged, Szeged, Hungary.
Benitez
Cecil
C
Takács
Sándor
S
Biessmann
Harald
H
D000818:Animals; D002843:Chromatin / Q000235:genetics / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics* / Q000378:metabolism; D002871:Chromosome Banding; D017931:DNA Primers; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics*; D015683:Gene Products, gag / Q000235:genetics / Q000378:metabolism; D008957:Models, Genetic; D010053:Ovary / Q000378:metabolism; D011485:Protein Binding; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D018626:Retroelements / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016615:Telomere / Q000235:genetics / Q000378:metabolism*
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D017931:DNA Primers; D029721:Drosophila Proteins; D015683:Gene Products, gag; C103996:HeT-A-gag protein, Drosophila; D012097:Repressor Proteins; D018626:Retroelements; C103826:prod protein, Drosophila