title: Solvent resistant microfluidic DNA synthesizer.
abstract: We fabricated a microfluidic DNA synthesizer out of perfluoropolyether (PFPE), an elastomer with excellent chemical compatibility which makes it possible to perform organic chemical reactions, and synthesized 20-mer oligonucleotides on chip.
authors.affiliation: Department of Bioengineering, Stanford University and Howard Hughes Medical Institute, California 94305, USA.
authors.forename: Yanyi
authors.initials: Y
authors.lastname: Huang
authors.forename: Piero
authors.initials: P
authors.lastname: Castrataro
authors.forename: Cheng-Chung
authors.initials: CC
authors.lastname: Lee
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Quake
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000138:chemical synthesis*; D020724:Elastomers / Q000737:chemistry; D004987:Ethers / Q000737:chemistry; D005466:Fluorocarbons / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D009841:Oligonucleotides / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry*
chemical_list: D020724:Elastomers; D004987:Ethers; D005466:Fluorocarbons; D009841:Oligonucleotides; D012997:Solvents; C078113:perfluoropolyether; D004247:DNA