title: Biomarkers for isocyanate exposure: Synthesis of isocyanate DNA adducts.
abstract: Isocyanates are important intermediates in industrial manufacturing. DNA adducts and protein adducts are important tools to biomonitor people exposed to xenobiotics. In the present work, the formation of DNA adducts deriving from 4-chlorophenyl isocyanate (4CPI) and 4-methylphenyl isocyanate (4MPI) were explored. The adducts of 4CPI and/or 4MPI with 2'-deoxyadenosine, 2'-deoxyguanosine, and 2'-deoxycytidine were synthesized and characterized by NMR and MS. For low level detection, an LC-MS/MS method was developed. The formation of DNA adducts was confirmed in in vitro reactions with DNA.
authors.affiliation: Institute of Environmental and Occupational Toxicology, Casella Postale 108, CH-6780 Airolo, Switzerland.
authors.forename: Armin
authors.initials: A
authors.lastname: Beyerbach
authors.forename: Peter B
authors.initials: PB
authors.lastname: Farmer
authors.forename: Gabriele
authors.initials: G
authors.lastname: Sabbioni
mesh_terms: D015415:Biomarkers / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D018736:DNA Adducts / Q000138:chemical synthesis / Q000737:chemistry; D004781:Environmental Exposure / Q000032:analysis*; D017953:Isocyanates / Q000737:chemistry*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D009705:Nucleosides / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D015415:Biomarkers; D018736:DNA Adducts; D017953:Isocyanates; D009705:Nucleosides