title: Characterization of mouse tissue kallikrein 5.
abstract: Mouse tissue kallikreins (Klks) are members of a large, multigene family consisting of 37 genes, 26 of which can code for functional proteins. Mouse tissue kallikrein 5 (Klk5) has long been thought to be one of these functional genes, but the gene product, mK5, has not been isolated and characterized. In the present study, we prepared active recombinant mK5 using an Escherichia coli expression system, followed by column chromatography. We then determined the biochemical and enzymatic properties of purified mK5. mK5 had trypsin-like activity for Arg at the P1 position, and its activity was inhibited by typical serine protease inhibitors. mK5 degraded gelatin, fibronectin, collagen type IV, high-molecular-weight kininogen, and insulin-like growth factor binding protein-3. Our data suggest that mK5 may be implicated in the process of extracellular matrix remodeling.
authors.affiliation: Laboratory of Molecular and Cellular Interactions, Faculty of Advanced Life Science, Hokkaido University, Sapporo, Japan.
authors.forename: Sanath
authors.initials: S
authors.lastname: Rajapakse
authors.forename: Katsueki
authors.initials: K
authors.lastname: Ogiwara
authors.forename: Noriko
authors.initials: N
authors.lastname: Yamano
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kimura
authors.forename: Kensaku
authors.initials: K
authors.lastname: Hirata
authors.forename: Sumio
authors.initials: S
authors.lastname: Takahashi
authors.forename: Takayuki
authors.initials: T
authors.lastname: Takahashi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004926:Escherichia coli; D015870:Gene Expression; D007610:Kallikreins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D011994:Recombinant Proteins
chemical_list: D011994:Recombinant Proteins; D007610:Kallikreins; C495973:Klk5 protein, mouse