Evaluation of a boosted-p24 antigen assay for the early diagnosis of pediatric HIV-1 infection in Cambodia.
Methods for early diagnosis of pediatric HIV-1 infection (DNA-polymerase chain reaction [PCR], RNA quantification, viral cultures) are expensive. Most Cambodian infants wait 18 months for HIV serologic tests. We observed that boosted-p24-antigen profile assay, with performances similar to viral cultures and costs similar to DNA-PCR, is easier to perform and could readily be set up in resource-poor settings.
Nouhin
Janin
J
Institut Pasteur du Cambodge, Phnom Penh, Cambodia.
Nguyen
Marie
M
D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis*; D002160:Cambodia; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D016655:HIV Core Protein p24 / Q000032:analysis*; D015658:HIV Infections / Q000175:diagnosis*; D006801:Humans; D007223:Infant; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification
D004279:DNA, Viral; D016655:HIV Core Protein p24; D012367:RNA, Viral