title: Detection of respiratory pathogens by real-time PCR in children with clinical suspicion of pertussis.
abstract: The use of a multiplex respiratory real-time PCR in patients clinically suspected of pertussis increases the number of pathogens detected.
authors.affiliation: Department of Pediatrics, Center of Infectious Diseases, Leiden University Medical Center, P.O. Box 9600, 2300 RC, Leiden, The Netherlands.
authors.forename: Alida M
authors.initials: AM
authors.lastname: van Kruijssen
authors.forename: Kate E
authors.initials: KE
authors.lastname: Templeton
authors.forename: Roos N
authors.initials: RN
authors.lastname: van der Plas
authors.forename: H Rogier
authors.initials: HR
authors.lastname: van Doorn
authors.forename: Eric C J
authors.initials: EC
authors.lastname: Claas
authors.forename: Ram N
authors.initials: RN
authors.lastname: Sukhai
authors.forename: Ed J
authors.initials: EJ
authors.lastname: Kuijper
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification; D001886:Bordetella pertussis / Q000235:genetics / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014780:Viruses / Q000235:genetics / Q000302:isolation & purification; D014917:Whooping Cough / Q000175:diagnosis*