title: Postnatal deletion of Numb/Numblike reveals repair and remodeling capacity in the subventricular neurogenic niche.
abstract: Neural stem cells are retained in the postnatal subventricular zone (SVZ), a specialized neurogenic niche with unique cytoarchitecture and cell-cell contacts. Although the SVZ stem cells continuously regenerate, how they and the niche respond to local changes is unclear. Here we generated nestin-creER(tm) transgenic mice with inducible Cre recombinase in the SVZ and removed Numb/Numblike, key regulators of embryonic neurogenesis from postnatal SVZ progenitors and ependymal cells. This resulted in severe damage to brain lateral ventricle integrity and identified roles for Numb/Numblike in regulating ependymal wall integrity and SVZ neuroblast survival. Surprisingly, the ventricular damage was eventually repaired: SVZ reconstitution and ventricular wall remodeling were mediated by progenitors that escaped Numb deletion. Our results show a self-repair mechanism in the mammalian brain and may have implications for both niche plasticity in other areas of stem cell biology and the therapeutic use of neural stem cells in neurodegenerative diseases.
authors.affiliation: Howard Hughes Medical Institute, Departments of Physiology and Biochemistry, University of California, San Francisco, CA 94143, USA.
authors.forename: Chay T
authors.initials: CT
authors.lastname: Kuo
authors.forename: Zaman
authors.initials: Z
authors.lastname: Mirzadeh
authors.forename: Mario
authors.initials: M
authors.lastname: Soriano-Navarro
authors.forename: Mladen
authors.initials: M
authors.lastname: Rasin
authors.forename: Denan
authors.initials: D
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Shen
authors.forename: Nenad
authors.initials: N
authors.lastname: Sestan
authors.forename: Jose
authors.initials: J
authors.lastname: Garcia-Verdugo
authors.forename: Arturo
authors.initials: A
authors.lastname: Alvarez-Buylla
authors.forename: Lily Y
authors.initials: LY
authors.lastname: Jan
authors.forename: Yuh-Nung
authors.initials: YN
authors.lastname: Jan
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000473:pathology / Q000502:physiology; D002450:Cell Communication; D004805:Ependyma / Q000033:anatomy & histology / Q000166:cytology / Q000502:physiology*; D005260:Female; D017353:Gene Deletion; D019426:Integrases / Q000235:genetics; D007381:Intermediate Filament Proteins / Q000235:genetics; D047908:Intracellular Signaling Peptides and Proteins; D020547:Lateral Ventricles / Q000502:physiology*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000502:physiology*; D064231:Nestin; D009474:Neurons / Q000502:physiology*; D013234:Stem Cells / Q000502:physiology*
chemical_list: D007381:Intermediate Filament Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; C106293:Numb protein, mouse; C106292:Numbl protein, mouse; C045073:Cre recombinase; D019426:Integrases