title: Detection of epidermal growth factor receptor (EGFR) mutations in non-small cell lung cancer (NSCLC) using a fully automated system with a nano-scale engineered biomagnetite.
abstract: A fully automated system using nano-scale engineered biomagnetite was developed to detect mutations in the epidermal growth factor receptor (EGFR) gene in non-small cell lung cancer (NSCLC). Bacterial magnetic particles (BacMPs) were isolated from the magnetic bacterium Magnetospirillum magneticum AMB-1 and conjugated to streptavidin. Biotin-labeled target PCR products were then captured with the BacMPs, hybridized with the detection probe and detected by fluorescence signaling. The process was performed using a newly designed automated processor equipped with an XYZ mobile arm containing a 96-way automated pipetter, reagent dispenser and fluorescence detector. Two types of somatic mutations (in-frame deletions and point substitutions) in the EGFR gene were successfully identified within 3.5h using this system, suggesting that this system could be used in clinical tests of EGFR gene mutations in lung cancer, and potentially other cancer, patients. Additionally, a very low mutation rate could be detected in these samples.
authors.affiliation: Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Maruyama
authors.forename: Haruko
authors.initials: H
authors.lastname: Takeyama
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Mori
authors.forename: Keiichi
authors.initials: K
authors.lastname: Ohshima
authors.forename: Shun-Ichiro
authors.initials: S
authors.lastname: Ogura
authors.forename: Toru
authors.initials: T
authors.lastname: Mochizuki
authors.forename: Tadashi
authors.initials: T
authors.lastname: Matsunaga
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D045744:Cell Line, Tumor; D004252:DNA Mutational Analysis / Q000295:instrumentation; D066246:ErbB Receptors / Q000235:genetics*; D052203:Ferrosoferric Oxide; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D036103:Nanotechnology
chemical_list: D066246:ErbB Receptors; D052203:Ferrosoferric Oxide