Microsomal and cytosolic biotransformation of aflatoxin B1 in four poultry species.
1. This research evaluated differences in hepatic in vitro metabolism of aflatoxin B1 (AFB1) on selected avian species. 2. Microsomal and cytosolic liver fractions were obtained from chickens, ducks, quails and turkeys; eight males and eight females of each. 3. All microsomes studied produced AFB1-8,9-exo-epoxide (AFBO), a metabolite regarded as the active product of AFB1. Turkey microsomes produced 1.8 and 3.5 times more AFBO than quails and chickens microsomes, respectively. 4. Males from evaluated birds produced more AFBO than females, but statistically-significant differences between genders were observed only in ducks and turkeys. 5. The cytosolic fraction from all four species produced aflatoxicol (AFL). Turkey and duck hepatic cytosol produced more AFL than from quail and chickens. 6. It is known that turkeys are very sensitive to AFB1, quails are intermediate and chickens are particularly resistant; the differences in AFBO production shown in our study may help to explain the difference in vivo responses among turkeys, quail and chickens. 7. Moreover, AFL may be related to AFB1 toxicity; it was produced in larger amounts by hepatic cytosol from the more susceptible species. 8. Because AFBO production by microsomes in ducks was relatively low, it is possible that other toxicity mechanisms are involved in this highly susceptible species.
Lozano
M C
MC
Departamento de Farmacia, Universidad Nacional de Colombia, Bogotá, Colombia.
Diaz
G J
GJ
D016604:Aflatoxin B1 / Q000378:metabolism*; D000818:Animals; D001711:Biotransformation; D002645:Chickens / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism*; D004372:Ducks / Q000378:metabolism*; D005260:Female; D022781:Hepatocytes / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D011784:Quail / Q000378:metabolism*; D013045:Species Specificity; D014422:Turkeys / Q000378:metabolism*
D016604:Aflatoxin B1