title: Localization and expression of selenoprotein S in the testis of Psammomys obesus.
abstract: Selenium is an essential trace element and selenoprotein S is a member of the selenoprotein family that has the non-standard amino acid selenocysteine incorporated into the polypeptide. Dietary selenium has been shown to play an important protective role in a number of diseases including cancer, immune function and the male reproductive system. In this study, we have observed high levels of selenoprotein S gene expression in the testis from Psammomys obesus. Real-time PCR and immunofluorescence demonstrate that selenoprotein S expression is low in testes from 4-week-old animals but increases significantly by 8 weeks of age and remains high until 17 weeks of age. Selenoprotein S protein is detected in primary spermatocytes, Leydig and Sertoli cells of 8, 12 and 17-week-old animals. These results suggest that selenoprotein S may play a role in spermatogenesis.
authors.affiliation: Metabolic Research Unit, School of Exercise and Nutrition Sciences, Deakin University, Pigdons Road, Waurn Ponds, VIC 3217, Australia. kellyw@deakin.edu.au
authors.forename: Kelly
authors.initials: K
authors.lastname: Windmill
authors.forename: Janette
authors.initials: J
authors.lastname: Tenne-Brown
authors.forename: Richard
authors.initials: R
authors.lastname: Bayles
authors.forename: James
authors.initials: J
authors.lastname: Trevaskis
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gao
authors.forename: Ken
authors.initials: K
authors.lastname: Walder
authors.forename: Greg R
authors.initials: GR
authors.lastname: Collier
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D019587:Dietary Supplements; D005849:Gerbillinae / Q000378:metabolism*; D008297:Male; D012643:Selenium / Q000494:pharmacology; D017279:Selenocysteine / Q000378:metabolism; D051140:Selenoproteins / Q000378:metabolism*; D013091:Spermatogenesis / Q000187:drug effects / Q000502:physiology*; D013737:Testis / Q000166:cytology / Q000473:pathology*
chemical_list: D051140:Selenoproteins; D017279:Selenocysteine; D012643:Selenium