[Protein kinase C isoforms in corneal epithelium and endothelium].
BACKGROUND
Protein kinase C (PKC) plays a key role in cell metabolism. Three subgroups and 12 isoforms have been isolated so far, catalysing specific functions in cell metabolism. The demonstration of PKC subtypes in corneal tissue has been inconsistent. The aim of this study was to verify the expression of several PKC subgroups and isoforms in human and bovine corneal epithelial and endothelial cells.


METHODS
PKC subgroups and isoforms were studied using polyclonal antibodies.


RESULTS
Antibodies to PKC-alpha, -delta, -epsilon and -zeta, representing all three PKC subgroups, bound in human and bovine corneal epithelium and endothelium. No binding was found for antibodies to PKC-beta2.


CONCLUSIONS
For the first time the presence of all three PKC subgroups was demonstrated in human and bovine corneal epithelial and endothelial cells. Further studies are needed to show the role of these subgroups in cellular functions such as cell proliferation and differentiation.
Grüb
M
M
Universitätsaugenklinik Tübingen. matthias-grueb@yahoo.de
El-Wardani
M
M
Mielke
J
J
Reinthal
E
E
Bartz-Schmidt
K U
KU
Rohrbach
J M
JM
Martin
J
J
D000328:Adult; D000368:Aged; D000818:Animals; D002417:Cattle; D004728:Endothelium, Corneal / Q000201:enzymology* / Q000473:pathology; D019573:Epithelium, Corneal / Q000201:enzymology* / Q000473:pathology; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011493:Protein Kinase C / Q000378:metabolism*; D012016:Reference Values
D007527:Isoenzymes; D011493:Protein Kinase C