title: Ultrastructure and gene mapping of the albino mutant al12 in rice (Oryza sativa L.).
abstract: Seedling albino mutation resistant to low temperature is an adaptability of rice (Oryza sativa L.) to cold. The mutant, a conditional expression controlled by development and temperature, differs from other albino mutants. The chlorophyll content of the mutant was measured using a portable chlorophyll meter, and the ultrastructure of the chloroplast was observed using a transmission electron microscope. Chlorophyll content was 1.2 SPAD, and the chloroplast did not develop, with only small vesicle-like structures. A segregation analysis of the reciprocal crosses between the albino mutation line with the rice line 9311 demonstrated that the albino trait was controlled by a single recessive gene, which was flanked by SSR markers RM5068 and RM3702 on the short arm of chromosome 8 with a distance of 0.5-1.1 cM and 4.9 cM, respectively. This gene was mapped within a 6 cM interval region and was tentatively referred to as al12.
authors.affiliation: Rice Institute of Sichuan Agricultural University, Wenjiang 611130, China.
authors.forename: Jiu-Cheng
authors.initials: JC
authors.lastname: Xia
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Wang
authors.forename: Bing-Tian
authors.initials: BT
authors.lastname: Ma
authors.forename: Zhao-Qing
authors.initials: ZQ
authors.lastname: Yin
authors.forename: Ming
authors.initials: M
authors.lastname: Hao
authors.forename: De-Wei
authors.initials: DW
authors.lastname: Kong
authors.forename: Shi-Gui
authors.initials: SG
authors.lastname: Li
mesh_terms: D000220:Adaptation, Biological / Q000235:genetics; D002734:Chlorophyll / Q000378:metabolism; D002736:Chloroplasts / Q000648:ultrastructure; D002874:Chromosome Mapping; D003080:Cold Temperature; D017343:Genes, Plant; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000502:physiology / Q000648:ultrastructure*; D010641:Phenotype; D010858:Pigmentation / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D036226:Seedlings / Q000235:genetics / Q000502:physiology
chemical_list: D002734:Chlorophyll