title: Identification of nine new HLA class I alleles in volunteers from the Singapore stem cell donor registries.
abstract: Based on unusual probe hybridization patterns, two human leukocyte antigen (HLA)-A, five HLA-B, and two HLA-C alleles (A*240208, A*3211Q; B*1822, B*3938Q, B*4606, B*4607N, B*5139; Cw*0321, Cw*0734) were identified in individuals from the Singapore Bone Marrow Donor Program and Singapore Cord Blood Bank. Eight of the nine alleles encode amino acid substitutions altering the antigen-binding region including three alleles with changes altering a cysteine at codon 164 (A*3211Q, B*3938Q, B*4607N). This substitution either eliminates a key disulfide bond or results in a stop codon, both likely affecting the expression of the HLA molecules. Only one allele (A*240208) carries a synonymous substitution.
authors.affiliation: Department of Pediatrics, Georgetown University Medical Center, Washington, DC, USA.
authors.forename: T F
authors.initials: TF
authors.lastname: Tang
authors.forename: L
authors.initials: L
authors.lastname: Hou
authors.forename: B
authors.initials: B
authors.lastname: Tu
authors.forename: W Y K
authors.initials: WY
authors.lastname: Hwang
authors.forename: A E J
authors.initials: AE
authors.lastname: Yeoh
authors.forename: J
authors.initials: J
authors.lastname: Ng
authors.forename: C K
authors.initials: CK
authors.lastname: Hurley
mesh_terms: D000483:Alleles; D018070:Biological Specimen Banks; D005091:Exons; D006680:HLA Antigens / Q000235:genetics*; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D012042:Registries; D012846:Singapore; D033581:Stem Cell Transplantation; D014019:Tissue Donors
chemical_list: D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I