Analysis of plasmids encoding extended-spectrum beta-lactamases (ESBLs) from Escherichia coli isolated from non-hospitalised patients in Seville.
Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli is an emerging pathogen in the community. We investigated the specific types of ESBLs in E. coli isolates from non-hospitalised patients and the plasmids harbouring these ESBLs. Forty-eight consecutive ESBL-producing E. coli were studied. All the isolates were clonally unrelated. ESBLs were characterised by sequencing. Mobility of the undigested plasmids was investigated both by conventional electrophoresis and by pulsed-field gel electrophoresis. Southern restriction fragment length polymorphism (RFLP) experiments were carried out by digesting plasmids with EcoRI and HindIII. CTX-M-14 was the most frequent ESBL (28 isolates), followed by SHV-12 (16 isolates). Plasmids encoding CTX-M-14 appeared homogeneous both in hybridisation profile and mobility. In contrast, plasmids expressing SHV-12 were more varied in size and hybridisation patterns. Our data suggest that a single type or very closely related plasmids harbouring CTX-M-14 are spreading in the community among unrelated E. coli isolates in our area.
Velasco
Carmen
C
Department of Microbiology, Universidad de Sevilla, Spain. cvelasco@us.es
Romero
Luisa
L
Martínez
José Manuel Rodríguez
JM
Rodríguez-Baño
Jesús
J
Pascual
Alvaro
A
D015139:Blotting, Southern; D004269:DNA, Bacterial / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics; D006801:Humans; D010045:Outpatients; D010957:Plasmids / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D013030:Spain / Q000453:epidemiology; D001618:beta-Lactamases / Q000235:genetics*
D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C412613:CTX-M-9 protein, E coli; C456080:beta-lactamase CTX-M-14; C107198:beta-lactamase SHV-12; D001618:beta-Lactamases