title: Radioprotective effects of hawthorn fruit extract against gamma irradiation in mouse bone marrow cells.
abstract: The radioprotective effect of hawthorn (Crataegus microphylla) fruit extract against genotoxicity induced by gamma irradiation has been investigated in mouse bone marrow cells. A single intraperitoneal (ip) administration of hawthorn extract at doses of 25, 50, 100 and 200 mg/kg 1h prior to gamma irradiation (2 Gy) reduced the frequencies of micronucleated polychromatic erythrocytes (MnPCEs). All four doses of hawthorn extract significantly reduced the frequencies of MnPCEs and increased the PCE/PCE+NCE ratio (polychromatic erythrocyte/ polychromatic erythrocyte + normochromatic erythrocyte) in mice bone marrow compared with the non drug-treated irradiated control (p < 0.02-0.00001). The maximum reduction in MnPCEs was observed in mice treated with extract at a dose of 200 mg/kg. Administration of amifostine at dose 100 mg/kg and hawthorn at dose 200 mg/kg reduced the frequency of MnPCE almost 4.8 and 5.7 fold; respectively, after being exposed to 2 Gy of gamma rays, compare with the irradiated control group. Crataegus extract exhibited concentration-dependent activity on 1,1-diphenyl 2-picrylhydrazyl free radical showing that Crataegus contained high amounts of phenolic compounds and the HPLC analysis determined that it contained chlorogenic acid, epicatechin and hyperoside. It appeared that hawthorn extract with antioxidant activity reduced the genotoxicity induced by gamma irradiation in bone marrow cells.
authors.affiliation: Department of Medicinal Chemistry, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran. sjhosseinim@yahoo.com
authors.forename: Seyed Jalal
authors.initials: SJ
authors.lastname: Hosseinimehr
authors.forename: Mohammad
authors.initials: M
authors.lastname: Azadbakht
authors.forename: Seyedeh Maryam
authors.initials: SM
authors.lastname: Mousavi
authors.forename: Aziz
authors.initials: A
authors.lastname: Mahmoudzadeh
authors.forename: Shahram
authors.initials: S
authors.lastname: Akhlaghpoor
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology* / Q000528:radiation effects*; D002478:Cells, Cultured; D027825:Crataegus / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D005638:Fruit / Q000737:chemistry*; D005720:Gamma Rays; D051379:Mice; D048629:Micronuclei, Chromosome-Defective / Q000528:radiation effects; D010936:Plant Extracts / Q000008:administration & dosage*; D011829:Radiation Dosage; D011836:Radiation Tolerance / Q000187:drug effects*; D011837:Radiation-Protective Agents / Q000008:administration & dosage*
chemical_list: D010936:Plant Extracts; D011837:Radiation-Protective Agents