title: Structural basis for modulation of Kv4 K+ channels by auxiliary KChIP subunits.
abstract: KChIPs coassemble with pore-forming Kv4 alpha subunits to form a native complex in the brain and heart and regulate the expression and gating properties of Kv4 K(+) channels, but the mechanisms underlying these processes are unknown. Here we report a co-crystal structure of the complex of human Kv4.3 N-terminus and KChIP1 at a 3.2-A resolution. The structure reveals a unique clamping action of the complex, in which a single KChIP1 molecule, as a monomer, laterally clamps two neighboring Kv4.3 N-termini in a 4:4 manner, forming an octamer. The proximal N-terminal peptide of Kv4.3 is sequestered by its binding to an elongated groove on the surface of KChIP1, which is indispensable for the modulation of Kv4.3 by KChIP1, and the same KChIP1 molecule binds to an adjacent T1 domain to stabilize the tetrameric Kv4.3 channels. Taken together with biochemical and functional data, our findings provide a structural basis for the modulation of Kv4 by KChIPs.
authors.affiliation: National Institute of Biological Sciences, No. 7 Science Park Road, Beijing 102206, China.
authors.forename: Huayi
authors.initials: H
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Yan
authors.forename: Qun
authors.initials: Q
authors.lastname: Liu
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Huang
authors.forename: Yue
authors.initials: Y
authors.lastname: Shen
authors.forename: Linjie
authors.initials: L
authors.lastname: Chen
authors.forename: Yi
authors.initials: Y
authors.lastname: Chen
authors.forename: Qiuyue
authors.initials: Q
authors.lastname: Yang
authors.forename: Quan
authors.initials: Q
authors.lastname: Hao
authors.forename: KeWei
authors.initials: K
authors.lastname: Wang
authors.forename: Jijie
authors.initials: J
authors.lastname: Chai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D018360:Crystallography, X-Ray; D005998:Glycine / Q000378:metabolism; D006801:Humans; D015640:Ion Channel Gating; D051605:Kv Channel-Interacting Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D009865:Oocytes; D018408:Patch-Clamp Techniques / Q000379:methods; D011392:Proline / Q000378:metabolism; D017434:Protein Structure, Tertiary / Q000235:genetics; D021122:Protein Subunits / Q000502:physiology; D051672:Shal Potassium Channels / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D014982:Xenopus laevis
chemical_list: D051605:Kv Channel-Interacting Proteins; D021122:Protein Subunits; D051672:Shal Potassium Channels; D011392:Proline; D005998:Glycine