Identification of CLUAP1 as a human osteosarcoma tumor-associated antigen recognized by the humoral immune system.
Since the prognosis of human osteosarcoma in advanced stage remains poor, the development of new and effective therapies including immunotherapy is required. To identify tumor-associated antigens of osteosarcoma applicable to the immunotherapy of this malignancy, we employed the serological analysis of recombinant cDNA expression library (SEREX) technique that defines tumor antigens recognized by the humoral immune system. Screening a cDNA library derived from an osteosarcoma cell line MG63 with sera from osteosarcoma patients identified 43 positive clones, representing 14 distinct antigens. Among them, CLUAP1 (clusterin-associated protein 1) was highly expressed in osteosarcoma tissue samples and cell lines. Overexpression of CLUAP1 was observed in other malignancies including ovarian, colon, and lung cancers. Our results suggest that CLUAP1 may be useful as a prognostic/diagnostic marker and/or for a target of immunotherapy of osteosarcoma.
Ishikura
Hisamitsu
H
Division of Immunoregulation, Institute for Genetic Medicine, Hokkaido University, Sapporo 060-0815, Japan.
Ikeda
Hiroaki
H
Abe
Hiroyuki
H
Ohkuri
Takayuki
T
Hiraga
Hiroaki
H
Isu
Kazuo
K
Tsukahara
Tomohide
T
Sato
Noriyuki
N
Kitamura
Hidemitsu
H
Iwasaki
Norimasa
N
Takeda
Naoki
N
Minami
Akio
A
Nishimura
Takashi
T
D000293:Adolescent; D000951:Antigens, Neoplasm / Q000378:metabolism / Q000502:physiology*; D045744:Cell Line, Tumor; D002648:Child; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D006801:Humans; D007107:Immune System / Q000378:metabolism*; D008297:Male; D012516:Osteosarcoma / Q000276:immunology / Q000378:metabolism* / Q000628:therapy; D011379:Prognosis; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014018:Tissue Distribution
D000951:Antigens, Neoplasm; C518614:CLUAP1 protein, human; D018076:DNA, Complementary; D012333:RNA, Messenger; D011994:Recombinant Proteins