Effect of HbS in the determination of HbA(2) with the TOSOH HLC-723G7 analyzer and the HELENA Beta-Thal Quik column kit.
OBJECTIVE
The analytical performance of the TOSOH HLC-723G7 hemoglobin HPLC analyzer and the effect of the presence of HbS in the determination of HbA(2) using HPLC and manual column methods.


METHODS
The performance characteristics of the TOSOH HLC-723G7 analyzer in the determination of HbA(2) were compared to those of the HELENA Beta-Thal Quik column. The effect of HbS presence in the samples was quantified using the HELENA SAS-MX alkaline gel electrophoresis kit as the reference method.


RESULTS
Within-run and between-run CVs for HbA(2) were better for the TOSOH HPLC analyzer than for the HELENA manual column method. The presence of HbS in the samples produces a strong positive bias in the %HbA(2) values when using both the HPLC and manual column methods, compared to the alkaline electrophoresis gel.


CONCLUSIONS
Both the TOSOH HPLC and the manual column are reliable methods for %HbA(2) determination when no HbS is detectable in the samples. When HbS is present, the gel electrophoresis method gives more accurate results.
Kalleas
Christoforos
C
Haematology Laboratory, General Hospital of Poligiros, Poligiros, Greece.
Tentes
Ioannis
I
Margaritis
Dimitrios
D
Anagnostopoulos
Kostas
K
Toli
Athina
A
Pendilas
Dimitrios
D
Bourikas
Georgios
G
Tsatalas
Constantinos
C
Kortsaris
Alexandros H
AH
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000295:instrumentation* / Q000379:methods; D006443:Hemoglobin A2 / Q000032:analysis*; D006451:Hemoglobin, Sickle / Q000032:analysis*; D006801:Humans; D015203:Reproducibility of Results
D006451:Hemoglobin, Sickle; D006443:Hemoglobin A2