title: A folic acid-based functionalized surface for biosensor systems.
abstract: The performance of a biosensor depends largely on its interface with the biological system. This interface imparts a biologically relevant function to the device and provides a measure of specificity towards the biological analyte of interest. This paper documents the choice of folic acid as the functional component of a cantilever sensor to recognize nasopharyngeal (KB) cancer cells. A conjugation chemistry protocol has been outlined to deploy folic acid onto a titanium-coated sensor surface using a silane linker. The presence and biological activity of the sensor was verified by means of an immunospecific (ELISA) procedure. The overall performance of the folic acid-based cantilever sensor was measured using cancerous KB cell-binding experiments.
authors.affiliation: Department of Agricultural and Biological Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. bhalerao@uiuc.edu
authors.forename: Kaustubh D
authors.initials: KD
authors.lastname: Bhalerao
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Lee
authors.forename: Winston O
authors.initials: WO
authors.lastname: Soboyejo
authors.forename: Alfred B O
authors.initials: AB
authors.lastname: Soboyejo
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D045744:Cell Line, Tumor; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D005492:Folic Acid / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D015394:Molecular Structure; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism / Q000473:pathology; D011437:Propylamines; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012821:Silanes / Q000737:chemistry; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry
chemical_list: D020099:Coated Materials, Biocompatible; D011437:Propylamines; D012821:Silanes; D005492:Folic Acid; D014025:Titanium; C477625:amino-propyl-triethoxysilane