Diversity in the degree of sulfation and chain length of the glycosaminoglycan moiety of urinary trypsin inhibitor isomers.
Five isomers with different electric charge were fractionated from human urinary trypsin inhibitor (UTI) by anion exchange HPLC. Intact low-sulfated chondroitin 4-sulfate chains from the isomers were analyzed by HPLC and mass spectrometry. Unsaturated disaccharide composition analysis of the chondroitin sulfate chain revealed that the five isomers differ in the numbers of 4-sulfated disaccharide units. Intriguingly, we detected the presence of multiple novel isomers with different numbers of non-sulfated disaccharide units even in the same charge isomer fraction. Our results demonstrate that UTI can vary in terms of both the degree of sulfation and the length of the low-sulfated chondroitin 4-sulfate chain.
Kakizaki
Ikuko
I
Department of Biochemistry, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan.
Takahashi
Ryouki
R
Ibori
Nobuyuki
N
Kojima
Kaoru
K
Takahashi
Teruno
T
Yamaguchi
Masanori
M
Kon
Atushi
A
Takagaki
Keiichi
K
D002240:Carbohydrate Sequence; D002809:Chondroitin Sulfates / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004187:Disaccharides / Q000737:chemistry / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000737:chemistry* / Q000302:isolation & purification; D006025:Glycosaminoglycans / Q000737:chemistry*; D006801:Humans; D007536:Isomerism; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification; D013431:Sulfates / Q000032:analysis*
D004187:Disaccharides; D006023:Glycoproteins; D006025:Glycosaminoglycans; D009844:Oligosaccharides; D013431:Sulfates; D002809:Chondroitin Sulfates; C028665:urinastatin