Evaluation of DBM/AM composite as a graft substitute for posterolateral lumbar fusion.
Demineralized bone matrix (DBM) has been investigated as a bone graft substitute for spinal fusion with less morbidity. Various carriers have been added to DBM to enhance its handling characteristics. This study investigates the spinal fusion induced by a composite of DBM and acellular dermal matrix (AM) in comparison with autologous bone in an athymic rat spinal fusion model. Single-level intertransverse process fusions were performed in 60 athymic nude rats grafted with 2 mL/kg of DBM/AM composite, AM alone, or autologous bone. Fusion was assessed at 6 weeks by radiography, manual palpation, and histology. At 6 weeks, 70% of the animals from the DBM/AM composite group exhibited complete spine fusion, whereas 35% from the autologous bone group and 20% from AM group showed bridging with some gaps. The DBM/AM composite induced a significantly higher fusion rate than both the autologous bone and AM groups (p < 0.001) in all measured parameters. The current study demonstrated that using DBM/AM composite can have more robust fusion than autologous bone at 6 weeks in an athymic rat spinal fusion model.
Qiu
Qing-Qing
QQ
LifeCell Corporation, Branchburg, New Jersey 08876, USA.
Shih
Mei-Shu
MS
Stock
Kimberly
K
Panzitta
Teri
T
Murphy
Patricia A
PA
Roesch
Sana C
SC
Connor
Jerome
J
D000818:Animals; D018775:Bone Demineralization Technique; D001857:Bone Matrix / Q000737:chemistry*; D018786:Bone Substitutes / Q000737:chemistry*; D016025:Bone Transplantation; D008159:Lumbar Vertebrae / Q000166:cytology / Q000000981:diagnostic imaging / Q000601:surgery*; D011859:Radiography; D051381:Rats; D011923:Rats, Nude; D013123:Spinal Fusion
D018786:Bone Substitutes