Pleomorphic extra-renal manifestation of the glomerular podocyte marker podocalyxin in tissues of normal beagle dogs.
Podocalyxin (PC) was initially identified as a major sialoprotein on the apical surface of glomerular podocytes to perform the filtration barrier function. Later, it was reported to be expressed in endothelial cells, megakaryotes/platelets, and hemangioblasts, the common progenitor cells of the hematopoietic and endothelial cells. Recently, increasing numbers of reports have indicated that PC is not merely a molecule restricted at renal glomerulus, angiogenic or hematopoietic system. To further elucidate the expression pattern and address the possible physiological role of PC in adult mammals, we conducted an extensive study by immunohistochemistry and immunofluorescence staining on various tissues of healthy adult beagle dogs. By combinatory usage of two different anti-podocalyxin antibodies recognizing distinct epitopes in PC, we have demonstrated that (1) PC is expressed in renal tubules, mesothelium, myocardium, striated muscles in tongue, esophagus and extraocular region, myoepithelial cells in esophagus and salivary glands, neurons, and ependyma, etc.; (2) there are at least three forms of PC proteins, depending upon the accessibility of two different PC antibodies, expressed in different organs/systems; and (3) a particular form of PC is distributed in a vesicle-like compartment in certain organs/systems, such as the central nervous system.
Lin
Wei-Ling
WL
Department of Internal Medicine, National Taiwan University Hospital and National Taiwan University College of Medicine, No. 7, Chung-Shan S. Road, Taipei, 100, Taiwan.
Pang
Victor Fei
VF
Liu
Chen-Hsuan
CH
Chen
Jen-Yau
JY
Shen
Kuo-Fang
KF
Lin
Yu-Yu
YY
Yu
Chun-Ying
CY
Hsu
Yung-Ho
YH
Jou
Tzuu-Shuh
TS
D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000737:chemistry; D002460:Cell Line; D045744:Cell Line, Tumor; D004064:Digestive System / Q000737:chemistry; D004285:Dogs; D004703:Endocrine System / Q000737:chemistry; D005123:Eye / Q000737:chemistry; D005260:Female; D005836:Genitalia, Female / Q000737:chemistry; D005837:Genitalia, Male / Q000737:chemistry; D006801:Humans; D007107:Immune System / Q000737:chemistry; D007150:Immunohistochemistry; D007678:Kidney Glomerulus / Q000737:chemistry* / Q000166:cytology; D008297:Male; D009206:Myocardium / Q000737:chemistry; D009420:Nervous System / Q000737:chemistry; D050199:Podocytes / Q000737:chemistry* / Q000166:cytology; D012795:Sialoglycoproteins / Q000032:analysis*; D014551:Urinary Tract / Q000737:chemistry
D015415:Biomarkers; D012795:Sialoglycoproteins; C001643:podocalyxin