A ventricular pressure-clamping system for the study of mechano-electrical feedback.
It is necessary to control the mechanical stimuli precisely in the studies of cardiac mechano-electrical feedback (MEF). In the present study a ventricular pressure-clamping system has been developed, which can be applied to isolated-perfused rabbit hearts. Controlled by a computer, this system not only can make the left ventricle follow a command defining the same pressure wave as that during a beating cycle under physiological condition, but also deliver mechanical stimuli with a proper waveform to the ventricle at a particular time phase. This system integrates multiple functions, including perfusing, pacing, recording of electrocardiogram and monophasic action potentials, and clamping and measuring of ventricular pressures in isolated-perfused hearts. Thus, it is a distinct system for investigating the phenomena and mechanisms of cardiac MEF at organ level.
Wei
Hua
H
Department of Physiology, Capital Medical University, Beijing 100069, China.
Huang
Hai-Xia
HX
Wang
Wei
W
Zhang
Zhi-Fei
ZF
Fu
Xiao-Suo
XS
Liu
Ping
P
Niu
Wei-Zhen
WZ
D000200:Action Potentials; D000818:Animals; D003250:Constriction; D004562:Electrocardiography; D005246:Feedback; D006321:Heart / Q000502:physiology*; D066298:In Vitro Techniques; D011817:Rabbits; D017725:Ventricular Pressure