Multianalyte profiling of serum cytokines for detection of pancreatic cancer.
Early detection of pancreatic cancer might improve clinical outcome. Significant alterations in the levels of individual serum cytokines have been reported in pancreatic cancer. We hypothesized that a multicytokine panel could serve as biomarkers for pancreatic cancer. To evaluate the diagnostic utility of such a panel, we have utilized a novel multianalyte LabMAP profiling technology that allows simultaneous measurement of multiple markers. In this study, a panel of 31 serological markers including cytokines, chemokines, growth and angiogenic factors in combination with CA 19-9 was analyzed in sera of pancreatic cancer patients, patients with chronic pancreatitis, and matched control healthy subjects. Statistical analysis identified a multicytokine panel that was able to distinguish pancreatic cancer from healthy controls with a sensitivity of 85.7% and specificity of 92.3%, which was superior to performance of CA 19-9 alone. Importantly, a multicytokine panel allowed the discrimination of pancreatic cancer from chronic pancreatitis with high sensitivity of 98% and specificity of 96.4%. In conclusion, we demonstrated that analysis of multiple serum cytokines using a novel LabMAP technology is a promising approach for development of a diagnostic assay for pancreatic cancer.
Zeh
H J
HJ
Division of Surgical Oncology, University of Pittsburgh, Pittsburgh, PA 15213, USA.
Winikoff
S
S
Landsittel
D P
DP
Gorelik
E
E
Marrangoni
A M
AM
Velikokhatnaya
L
L
Winans
M T
MT
Lee
K
K
Moser
A
A
Bartlett
D
D
Lotze
M T
MT
Siegfried
J M
JM
Whitcomb
D
D
Papacristou
G
G
Slivka
A
A
Bigbee
W L
WL
Lokshin
A E
AE
D014408:Biomarkers, Tumor / Q000097:blood*; D018395:CA-19-9 Antigen / Q000097:blood; D016022:Case-Control Studies; D035061:Control Groups; D016207:Cytokines / Q000097:blood*; D003627:Data Interpretation, Statistical; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D008297:Male; D010190:Pancreatic Neoplasms / Q000097:blood / Q000175:diagnosis*; D050500:Pancreatitis, Chronic / Q000097:blood / Q000175:diagnosis; D040081:Protein Array Analysis / Q000379:methods*; D012680:Sensitivity and Specificity
D014408:Biomarkers, Tumor; D018395:CA-19-9 Antigen; D016207:Cytokines