title: Aggregation of cyclodextrins as an important factor to determine their complexation behavior.
abstract: Here, we report a study on the complexation behavior of carotenoids with cyclodextrins (CDs) using solubility experiments and molecular-modelling methods. Carotenoids are an important group of naturally occurring dyes found in vegetables and fruits. Their antioxidant property has initiated investigations on their possible use as drugs. However, carotenoids are lipophilic molecules with very little inherent aqueous solubility. Cyclodextrin complexation has been widely used in order to increase the potential applications of hydrophobic compounds. Thus, the aim of our investigation was to design carotenoids with enhanced water solubility by cyclodextrin complexation. Molecular modelling of carotenoid-cyclodextrin complexes with a 1 : 1 stoichiometry successfully explained the experimentally observed capability of beta-cyclodextrins (beta-CDs) to form complexes with carotenoids as opposed to alpha-cyclodextrins (alpha-CDs) and gamma-cyclodextrins (gamma-CDs). Furthermore, molecular-dynamics calculations revealed that the aggregation properties of CD derivatives significantly influence their complexation behavior. Our docking calculations showed that RAMEB (random methylated beta-CD) is the beta-CD derivative that possesses the lowest tendency to aggregate. Solubility experiments yielded the same results, namely, RAMEB complexes possess the best water solubility. Our results showed that complexation of a ligand not buried inside of the CD cavity is dependent on two factors: i) the geometry of the inclusion part of the complex; ii) the self-aggregation property of the CD itself. The lower affinity the CDs possess for self-aggregation, the more likely are they involved in interactions with carotenoids. These results suggest that self-aggregation of CDs should be considered as an important parameter determining complexation in general.
authors.affiliation: Virtua Drug, Ltd., Csalogany st. 4c, H-1015 Budapest.
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Bikádi
authors.forename: Róbert
authors.initials: R
authors.lastname: Kurdi
authors.forename: Sándor
authors.initials: S
authors.lastname: Balogh
authors.forename: Julianna
authors.initials: J
authors.lastname: Szemán
authors.forename: Eszter
authors.initials: E
authors.lastname: Hazai
mesh_terms: D000975:Antioxidants / Q000737:chemistry; D002338:Carotenoids / Q000737:chemistry; D003198:Computer Simulation; D003505:Cyclodextrins / Q000737:chemistry*; D006801:Humans; D046911:Macromolecular Substances / Q000737:chemistry; D008956:Models, Chemical; D008958:Models, Molecular; D012995:Solubility; D013816:Thermodynamics; D014801:Vitamin A / Q000737:chemistry; D014867:Water / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry; D047408:gamma-Cyclodextrins / Q000737:chemistry
chemical_list: D000975:Antioxidants; D003505:Cyclodextrins; D046911:Macromolecular Substances; D047392:beta-Cyclodextrins; D047408:gamma-Cyclodextrins; D014867:Water; D014801:Vitamin A; D002338:Carotenoids; C031215:betadex; C023792:gamma-cyclodextrin