title: Gravity-induced absorption changes in Phycomyces blakesleeanus during parabolic flights: first spectral approach in the visible.
abstract: Gravity-induced absorption changes as experienced during a series of parabolas on the Airbus 300 Zero-G have been measured previously pointwise on the basis of dual-wavelength spectroscopy. Only the two wavelengths of 460 and 665 nm as generated by light-emitting diodes have been utilised during our first two parabolic-flight campaigns. In order to gain complete spectral information throughout the wavelength range from 400 to 900 nm, a miniaturized rapid scan spectrophotometer was designed. The difference of spectra taken at 0 g and 1.8 g presents the first gravity-induced absorption change spectrum measured on wild-type Phycomyces blakesleeanus sporangiophores, exhibiting a broad positive hump in the visible range and negative values in the near infrared with an isosbestic point near 735 nm. The control experiment performed with the stiff mutant A909 of Phycomyces blakesleeanus does not show this structure. These results are in agreement with those obtained with an array spectrophotometer. In analogy to the more thoroughly understood so-called light-induced absorption changes, we assume that gravity-induced absorption changes reflect redox changes of electron transport components such as flavins and cytochromes localised within the plasma membrane.
authors.affiliation: Fachbereich Biologie, Philipps-Universität, Marburg, Federal Republic of Germany. w.2.schmidt@surfeu.de
authors.forename: Werner
authors.initials: W
authors.lastname: Schmidt
mesh_terms: D000042:Absorption / Q000528:radiation effects; D003580:Cytochromes / Q000378:metabolism / Q000528:radiation effects; D004579:Electron Transport / Q000528:radiation effects; D004734:Energy Metabolism / Q000528:radiation effects*; D005415:Flavins / Q000378:metabolism / Q000528:radiation effects; D006112:Gravitation; D018522:Gravitropism / Q000528:radiation effects; D018466:Gravity Sensing / Q000528:radiation effects; D008027:Light; D009154:Mutation; D010084:Oxidation-Reduction / Q000528:radiation effects; D010800:Phycomyces / Q000378:metabolism* / Q000502:physiology / Q000528:radiation effects; D013026:Space Flight / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation; D013997:Time Factors; D014893:Weightlessness
chemical_list: D003580:Cytochromes; D005415:Flavins