title: Two-dimensional differential gel electrophoresis of rat heart proteins in ischemia and ischemia-reperfusion.
abstract: Ischemia-reperfusion injury occurs in acute myocardial infarction, cardiopulmonary bypass surgery, and heart transplantation. However the precise mechanisms still remain unclear. In order to identify proteins that are involved in ischemia-reperfusion injury, we compared precipitated 100,000g fractions of normal, ischemic, and ischemic-reperfused rat hearts using two-dimensional (2D) difference gel electrophoresis (2D-DIGE). 2D-DIGE is reliable method to define quantitative protein differences, especially when subtle protein changes are under investigation. In this study, six spots that changed more than twofold and two additional spots related to these spots were detected. Five of the spots were identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry as protein disulfide isomerase, one as 60 kDa heat-shock protein, and two as elongation factor Tu.
authors.affiliation: Sumitomo Pharmaceuticals, Osaka, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Sakai
authors.forename: Hironori
authors.initials: H
authors.lastname: Ishikawa
authors.forename: Hideshi
authors.initials: H
authors.lastname: Satoh
authors.forename: Setsuko
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Shinichi
authors.initials: S
authors.lastname: Kojima
authors.forename: Masaharu
authors.initials: M
authors.lastname: Kanaoka
mesh_terms: D000818:Animals; D018834:Chaperonin 60 / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional / Q000295:instrumentation / Q000379:methods*; D009203:Myocardial Infarction / Q000378:metabolism; D017202:Myocardial Ischemia / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D010444:Peptide Elongation Factor Tu / Q000032:analysis; D019704:Protein Disulfide-Isomerases / Q000032:analysis; D020543:Proteome / Q000032:analysis*; D051381:Rats; D015427:Reperfusion Injury / Q000378:metabolism*; D015203:Reproducibility of Results
chemical_list: D018834:Chaperonin 60; D020543:Proteome; D010444:Peptide Elongation Factor Tu; D019704:Protein Disulfide-Isomerases