title: Uptake of microparticles into the epithelium of human nasopharyngeal lymphoid tissue.
abstract: The M cells of nasopharyngeal lymphoid tissue (NALT) have been considered to play an important role for vaccine delivery systems in humans. A number of investigations have reported particle uptake data in NALT of rodents. However, there have been no reports indicating any involvement of the nasopharyngeal lymphoid tissue in human vaccination. In the present study, we investigated whether the epithelium of human adenoid tissues might incorporate fluorescent microparticles using electron and fluorescent microscopy. The dissected adenoid tissues were incubated with various sizes and concentrations of fluorescent microparticles for 120 min at 37 degrees C. Furthermore, the effect of surface coatings of microparticles with cations on the uptake into the epithelium of adenoid tissues was investigated. Transmission electron microscopy revealed that microparticles were taken up by the M cells of human nasopharyngeal lymphoid tissues. The NALT-M cells showed greater uptake of the smallest particles, 0.2 microm in diameter, than those of 0.5, 1.0, or 2.0 microm diameter. It was also revealed that surface coatings with poly-L: -lysin or chitosan resulted in efficient uptake into the NALT. These results indicate that nasal administration of antigenic microparticles, which were coated with cationic materials, probably leads to a useful method of transnasal vaccination against respiratory and intestinal infections in humans.
authors.affiliation: Department of Internal Medicine, Center for Gastroenterology and Endoscopy, Kawasaki Hospital, Kawasaki Medical School, 2-1-80 Nakasange, Okayama, 700-8505, Japan. fujimura@kawasaki-hp.jp
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Fujimura
authors.forename: Takeshi
authors.initials: T
authors.lastname: Akisada
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Harada
authors.forename: Ken
authors.initials: K
authors.lastname: Haruma
mesh_terms: D000234:Adenoids / Q000502:physiology / Q000648:ultrastructure; D001692:Biological Transport; D002412:Cations; D002675:Child, Preschool; D048271:Chitosan / Q000737:chemistry / Q000378:metabolism*; D016503:Drug Delivery Systems; D004848:Epithelium / Q000502:physiology / Q000648:ultrastructure; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D066298:In Vitro Techniques; D008221:Lymphoid Tissue / Q000502:physiology* / Q000648:ultrastructure; D008297:Male; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D008863:Microspheres; D009305:Nasopharynx / Q000502:physiology* / Q000648:ultrastructure; D010316:Particle Size; D011107:Polylysine / Q000737:chemistry / Q000378:metabolism*
chemical_list: D002412:Cations; D005456:Fluorescent Dyes; D011107:Polylysine; D048271:Chitosan