Analysis of gene function in rice through virus-induced gene silencing.
Virus-induced gene silencing (VIGS) is a powerful RNA-silencing based technology adapted for the study of host-gene function. VIGS functions through the expression of a host gene from a virus vector. Both the virus-encoded host sequence and the homologous host target messenger RNA are destroyed or made inactive through a host surveillance system. Here, we describe procedures for the use of a new virus vector for VIGS in monocotyledonous hosts and, in particular, in rice (Oryza sativa), a species for which no VIGS vector was previously available.
Ding
Xin Shun
XS
Division of Plant Biology, Samuel Roberts Noble Foundation, Ardmore, OK, USA.
Rao
C Srinivasa
CS
Nelson
Richard S
RS
D017795:Bromovirus / Q000472:pathogenicity / Q000502:physiology*; D015536:Down-Regulation; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D017343:Genes, Plant / Q000235:genetics* / Q000502:physiology; D005822:Genetic Vectors; D016254:Mutagenesis, Insertional; D012275:Oryza / Q000235:genetics* / Q000821:virology*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism*; D010957:Plasmids / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism; D014170:Transformation, Genetic
D010940:Plant Proteins; D012333:RNA, Messenger; D012367:RNA, Viral