Rapid quantification of bacterial cells in potable water using a simplified microfluidic device.
A simplified microfluidic device for quantification of bacteria in potable water was fabricated and examined. Comparisons of counts of Escherichia coli by the microfluidic system and by epifluorescence microscopy closely correlated (r2=0.99). Bacteria in natural mineral water and in purified household tap water were accurately enumerated by using this system within 15 min after fluorescent staining.
Sakamoto
Chieko
C
Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamada-oka, Suita, Osaka 565-0871, Japan.
Yamaguchi
Nobuyasu
N
Yamada
Masumi
M
Nagase
Hiroyasu
H
Seki
Minoru
M
Nasu
Masao
M
D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000302:isolation & purification*; D005434:Flow Cytometry; D005618:Fresh Water / Q000382:microbiology*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods; D008856:Microscopy, Fluorescence; D008900:Mineral Waters / Q000382:microbiology*; D013997:Time Factors; D014881:Water Supply
D008900:Mineral Waters