title: Modulating biochemical perturbations during 72-hour machine perfusion of kidneys: role of preservation solution.
abstract: This study documents renal biochemistry during hypothermic machine perfusion of kidneys. It is intended to demonstrate that a comprehensive evaluation of organ viability during ex-vivo preservation is needed to increase the number of organs available for transplantation and to reduce the current renal discard rate. Porcine kidneys were hypothermically machine perfused for 72 h with either Unisol-UHK or Belzer-Machine Perfusion Solution, (Belzer-MPS). Renal perfusate samples were periodically collected and biochemically analyzed. Significant differences were measured in the renal metabolic activity between the two experimental groups while similar values for traditional parameters such as renal flow rate and vascular resistance values were recorded. The effluent of UHK perfused kidneys showed strong metabolites and NH(4)(+) dynamics (P<0.05 vs. baseline), while the Belzer-MPS kidneys metabolic activity led to little or no change of the effluent biochemistry relative to baseline.
authors.affiliation: Cell and Tissue Systems, Inc., 2231 Technical Parkway, Suite A, N. Charleston, SC 29406, USA. sbaicu@celltissuesystems.com
authors.forename: Simona C
authors.initials: SC
authors.lastname: Baicu
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Taylor
authors.forename: Kelvin G M
authors.initials: KG
authors.lastname: Brockbank
mesh_terms: D000818:Animals; D007668:Kidney / Q000378:metabolism; D007677:Kidney Function Tests; D009926:Organ Preservation; D019188:Organ Preservation Solutions / Q000737:chemistry; D010477:Perfusion / Q000295:instrumentation*; D013552:Swine; D013997:Time Factors
chemical_list: D019188:Organ Preservation Solutions; C513328:UHK solution