Rapid species determination of Nocardia keratitis using pyrosequencing technology.
OBJECTIVE
To describe a new technique, pyrosequencing, which allows for the rapid identification of Mycobacterium and Nocardia species.


METHODS
Interventional case report.


METHODS
The medical records of a patient presenting with an infectious keratitis were reviewed.


RESULTS
A case of Nocardia abscessus/arthrititis/asiatica keratitis was diagnosed in a young individual with the aid of pyrosequencing technology. Based on presumed antibiotic sensitivities, therapy with topical trimethoprim-sulfamethoxazole eyedrops was initiated, and the infection was cleared rapidly with minimal residual scarring.


CONCLUSIONS
Pyrosequencing may be a useful tool in aiding the rapid diagnosis and treatment of ocular infections caused by slow-growing pathogens.
Galor
Anat
A
Cole Eye Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA.
Hall
Gerri S
GS
Procop
Gary W
GW
Tuohy
Marion
M
Millstein
Michael E
ME
Jeng
Bennie H
BH
D000328:Adult; D000890:Anti-Infective Agents / Q000627:therapeutic use; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis*; D015818:Eye Infections, Bacterial / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*; D006801:Humans; D007634:Keratitis / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*; D008297:Male; D008969:Molecular Sequence Data; D009615:Nocardia / Q000235:genetics* / Q000302:isolation & purification; D009617:Nocardia Infections / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*; D009883:Ophthalmic Solutions / Q000627:therapeutic use; D017422:Sequence Analysis, DNA / Q000379:methods*; D015662:Trimethoprim, Sulfamethoxazole Drug Combination / Q000627:therapeutic use
D000890:Anti-Infective Agents; D004269:DNA, Bacterial; D009883:Ophthalmic Solutions; D015662:Trimethoprim, Sulfamethoxazole Drug Combination