title: In vitro antioxidant studies of Sphaeranthus indicus (Linn).
abstract: The free radical scavenging potential of the plant S. indicus was studied by using different antioxidant models of screening. The ethanolic extract at 1000 microg/ml showed maximum scavenging of the radical cation, 2,2-azinobis-(3-ethylbenzothiazoline-6-sulphonate) (ABTS) observed upto 41.99% followed by the scavenging of the stable radical 1,1-diphenyl, 2-picryl hydrazyl (DPPH) (33.27%), superoxide dismutase (25.14%) and nitric oxide radical (22.36%) at the same concentration. However, the extract showed only moderate scavenging activity of iron chelation (14.2%). Total antioxidant capacity of the extract was found to be 160.85 nmol/g ascorbic acid. The results justify the therapeutic applications of the plant in the indigenous system of medicine, augmenting its therapeutic value.
authors.affiliation: Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal 576 104, India. annieshirwaikar@yahoo.com
authors.forename: Annie
authors.initials: A
authors.lastname: Shirwaikar
authors.forename: Kirti S
authors.initials: KS
authors.lastname: Prabhu
authors.forename: I S R
authors.initials: IS
authors.lastname: Punitha
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D066298:In Vitro Techniques; D009569:Nitric Oxide / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D013481:Superoxides; D009569:Nitric Oxide; D013482:Superoxide Dismutase