[Study on trans-differentiation of adult human myoblasts into neural precursor cells and its implantation in rats].
OBJECTIVE
To investigate the feasibility of inducing adult human myoblasts into neural precursor cells.


METHODS
The myoblasts were isolated with mixed digestive enzyme from minced human temporal muscle samples, cultured and purified clonally. The 3rd passage cells were incubated with serum free medium including basic fibroblast growth factor (bFGF), epidermal growth factor (EGF) and leukemia inhibitory factor (LIF). Morphological change was investigated during incubation period. Immunofluorescence cytochemistry and RT-PCR analysis were used to assess cell differentiation and trans differentiation.


RESULTS
After the induction, cells became non-adherent aggregates as neurospheres. The myoblast-derived neurospheres was immuno-positive for nestin. In differentiation condition, they looked like neurons and glial cells and expressed neuronal (microtubule associated protein 2, MAP-2), astrocytic (Glial fibrillary acidic protein, GFAP) and oligodendrocytic (Galactocerebroside, Galc) markers by immunocytochemistry. The result by RT-PCR was coincident with immunocytochemistry. The myoblast-derived neurospheres expressed MAP-2 and GFAP after they were transplanted into the brain of rats with cerebral ischemia.


CONCLUSIONS
Adult human myoblasts can be inducted to trans-differentiate into neural precursor cells.
Zhang
Zhen-xing
ZX
Department of Neurosurgery, Peking Union Medical College Hospital, Peking Union College and Chinese Academy of Medical Sciences, Beijing 100730, China.
Wang
Ren-zhi
RZ
Li
Gui-lin
GL
Dou
Wan-chen
WC
Li
Shi-fang
SF
Wei
Jun-ji
JJ
Wei
Yu-kui
YK
Zhao
Fei-fan
FF
Kong
Yan-guo
YG
Wu
Hai-tao
HT
Fan
Ming
M
D000328:Adult; D000818:Animals; D002454:Cell Differentiation; D048430:Cell Shape / Q000187:drug effects; D054338:Cell Transdifferentiation; D017690:Cell Transplantation; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D053605:Leukemia Inhibitory Factor / Q000494:pharmacology; D008297:Male; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis / Q000235:genetics; D032446:Myoblasts / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D009416:Nerve Regeneration; D009474:Neurons / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014183:Transplantation, Heterologous
D005904:Glial Fibrillary Acidic Protein; D053605:Leukemia Inhibitory Factor; D008869:Microtubule-Associated Proteins; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor