A bio-defensive function induced by low-dose lipopolysaccharide pre-treatment against CCl4-induced fulminant hepatic failure in rats.
We have investigated the bio-defensive function of low-dose LPS pretreatment on CCl4-induced fulminant hepatic failure (FHF) in rats. In male Donryu rats treated with CCl4 at 1.0 ml/kg i.p., the serum alanine aminotransferase (ALT) increased up to a peak at 24 h. The increase in intracellular Ca2+ concentration was biphasic, i.e., a first peak at 1 h and a second at 3 days. However, when rats were treated daily with CCl4 at 1.0 ml/kg, serum ALT successively increased over several h after the second administration, but the rats died of FHF. The first Ca2+ peak occurred 1 h after CCl4 administration, but the concentration did not increase continually, until it reached the second peak. However, rats did not die when they were treated with lipopolysaccharide at 0.5 mg/kg 24 h prior to the initial dose of CCl4 at 1.0 ml/kg. The serum ALT value did not increase after administration of CCl4 at 1.0 ml/kg, but the second Ca2+ peak was sustained at > 1, 000 nM. We concluded that the second Ca2+ peak acts to protect the liver from injury.
Ueda
Yukari
Y
Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Tokushima 770-8514, Japan.
Yabe
Kazunori
K
Taira
Zenei
Z
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002252:Carbon Tetrachloride Poisoning / Q000097:blood / Q000378:metabolism / Q000517:prevention & control; D004305:Dose-Response Relationship, Drug; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D017114:Liver Failure, Acute / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D008297:Male; D051381:Rats
D008070:Lipopolysaccharides; D000410:Alanine Transaminase; D002118:Calcium