title: Identification and characterization of a novel human collectin CL-K1.
abstract: Collectins are a family of C-type lectins with two characteristic structures, collagen like domains and carbohydrate recognition domains. They recognize carbohydrate antigens on microorganisms and act as host-defense. Here we report the cloning and characterization of a novel collectin CL-K1. RT-PCR analyses showed CL-K1 mRNA is present in all organs. The deduced amino acid sequence and the data from immunostaining of CL-K1 cDNA expressing CHO cells revealed that CL-K1 is expressed as a secreted protein. CL-K1 is found in blood by immunoblotting and partial amino acid analyses. CL-K1 showed Ca(2+)-dependent sugar binding activity of fucose and weakly mannose but not N-acetyl-galactosamine, N-acetyl-glucosamine, or maltose, though mannose-binding lectin (MBL) containing similar amino acid motif. CL-K1 can recognize specially several bacterial saccharides due to specific sugar-binding character. Elucidation of the role of two ancestor collectins of CL-K1 and CL-L1 could lead to see the biological function of collectin family.
authors.affiliation: Research & Development Center, Fuso Pharmaceutical Industries, Osaka, Osaka, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Keshi
authors.forename: Takashi
authors.initials: T
authors.lastname: Sakamoto
authors.forename: Takao
authors.initials: T
authors.lastname: Kawai
authors.forename: Katsuki
authors.initials: K
authors.lastname: Ohtani
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Katoh
authors.forename: Seong-Jae
authors.initials: SJ
authors.lastname: Jang
authors.forename: Wataru
authors.initials: W
authors.lastname: Motomura
authors.forename: Takayuki
authors.initials: T
authors.lastname: Yoshizaki
authors.forename: Mitsuko
authors.initials: M
authors.lastname: Fukuda
authors.forename: Satoshi
authors.initials: S
authors.lastname: Koyama
authors.forename: Jun
authors.initials: J
authors.lastname: Fukuzawa
authors.forename: Atsushi
authors.initials: A
authors.lastname: Fukuoh
authors.forename: Itsuro
authors.initials: I
authors.lastname: Yoshida
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Wakamiya
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D037222:Collectins / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D007668:Kidney / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data
chemical_list: C518521:Colec11 protein, human; D037222:Collectins