Materials specificity and directed assembly of a gold-binding peptide.
Adsorption studies of a genetically engineered gold-binding peptide, GBP1, were carried out using a quartz-crystal microbalance (QCM) to quantify its molecular affinity to noble metals. The peptide showed higher adsorption onto and lower desorption from a gold surface compared to a platinum substrate. The material specificity, that is, the preferential adsorption, of GBP1 was also demonstrated using gold and platinum micropatterned on a silicon wafer containing native oxide. The biotinylated three-repeat units of GBP1 were preferentially adsorbed onto gold regions delineated using streptavidin-conjugated quantum dots (SAQDs). These experiments not only demonstrate that an inorganic-binding peptide could preferentially adsorb onto a metal (Au) rather than an oxide (SiO2) but also onto one noble metal (Au) over another (Pt). This result shows the utility of an engineered peptide as a molecular erector in the directed immobilization of a nanoscale hybrid entity (SAQDs) over selected regions (Au) on a fairly complex substrate (Au and Pt micropatterned regions on silica). The selective and controlled adsorption of inorganic-binding peptides may have significant implications in nano- and nanobiotechnology, where they could be genetically tailored for specific use in the development of self-assembled molecular systems.
Tamerler
Candan
C
Materials Science & Engineering Department, Roberts Hall, Box: 352120, University of Washington, Seattle, WA 98195, USA.
Duman
Memed
M
Oren
Ersin Emre
EE
Gungormus
Mustafa
M
Xiong
Xiaorong
X
Kacar
Turgay
T
Parviz
Babak A
BA
Sarikaya
Mehmet
M
D000327:Adsorption; D019921:Biotinylation; D004305:Dose-Response Relationship, Drug; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D007700:Kinetics; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D036103:Nanotechnology / Q000379:methods*; D010455:Peptides / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry; D015202:Protein Engineering / Q000379:methods*; D045663:Quantum Dots; D013816:Thermodynamics
D010455:Peptides; D010984:Platinum; D006046:Gold