Molecular analysis of a full-length sequence of a new yam badnavirus from Dioscorea sansibarensis.
Badnavirus-like particles were observed by ISEM in viral preparations from yam (Dioscorea sansibarensis) leaves from Benin. Use of the viral preparation as template for PCR amplification with badnavirus-specific primers gave rise to a 579-bp product with most nucleotide identity (70.8%) to Dioscorea alata bacilliform virus (DaBV, Accession numbers X94575-X94582), the only other yam badnavirus sequenced to date. A full-length badnavirus sequence was generated, which consisted of 7261 nucleotides with a typical Badnavirus genome organisation. The full-length sequence shared most identity (61.9%) to DaBV (Accession numbers X94575-X94582) and hence represents a member of a new badnavirus species termed Dioscorea sansibarensis bacilliform virus (DsBV).
Seal
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Natural Resources Institute, The University of Greenwich at Medway, Chatham Maritime, UK. s.e.seal@gre.ac.uk
Muller
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D019195:Badnavirus / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D001541:Benin; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D027742:Dioscorea / Q000821:virology*; D023061:Gene Order; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D026801:Synteny
D004279:DNA, Viral