Transferability of short tandem repeat markers for two wild Canid species inhabiting the Brazilian Cerrado.
The maned wolf (Chrysocyon brachyurus) and the crab-eating fox (Cerdocyon thous) are two wild-canid species found in the Brazilian Cerrado. We tested cross-amplification and transferability of 29 short tandem repeat primers originally developed for cattle and domestic dogs and cats on 38 individuals of each of these two species, collected in the Emas National Park, which is the largest national park in the Cerrado region. Six of these primers were successfully transferred (CSSM-038, PEZ-05, PEZ-12, LOCO-13, LOCO-15, and PEZ-20); five of which were found to be polymorphic. Genetic parameter values (number of alleles per locus, observed and expected heterozygosities, and fixation indices) were within the expected range reported for canid populations worldwide.
Rodrigues
F M
FM
Departamento de Biologia, Universidade Estadual de Goiás, Anápolis, GO, Brasil. flavia.melo@ueg.br
Telles
M P C
MP
Resende
L V
LV
Soares
T N
TN
Diniz-Filho
J A F
JA
Jácomo
A T A
AT
Silveira
L
L
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D001938:Brazil; D002415:Cats; D002417:Cattle; D004285:Dogs; D005589:Foxes / Q000235:genetics*; D005819:Genetic Markers; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D019596:Wolves / Q000235:genetics*
D005819:Genetic Markers