Critical residues at the Ly49 natural killer receptor's homodimer interface determine functional recognition of m157, a mouse cytomegalovirus MHC class I-like protein.
NK cell function is regulated by Ly49 receptors in mice and killer cell Ig-like receptors in humans. Although inhibitory Ly49 and killer cell Ig-like receptors predominantly ligate classical MHC class I molecules, recent studies suggest that their activating counterparts recognize infection. The quintessential example is resistance to the mouse CMV in C57BL/6 mice, which depends on the functional recognition of m157, a mouse CMV-encoded MHC class I-like molecule, by Ly49H, an activating NK cell receptor. We have taken advantage of the natural variation in closely related members of the Ly49C-like receptors and the availability of Ly49 crystal structures to understand the molecular determinants of the Ly49H-m157 interaction and to identify amino acid residues discriminating between m157 binding and nonbinding receptors. Using a site-directed mutagenesis approach, we have targeted residues conserved in receptors binding to m157 (Ly49H and Ly49I(129)) but different from receptors lacking m157 recognition (Ly49C, Ly49I(B6), and Ly49U). Wild-type and mutant receptors were transfected into reporter cells, and physical binding as well as functional activation by m157 was studied. Our findings suggested that the Ly49 MHC class I contact "site 2," I226, may not be involved in m157 binding. In contrast, residue Y146 and G151, mapping at the receptor homodimer interface, are likely critical for functional recognition of the m157 glycoprotein. Our combined functional and three-dimensional modeling approach suggested that the architecture of the Ly49H dimer is crucial to accessing m157, but not MHC class I. These results link Ly49 homodimerization variability to the direct recognition of pathogen products.
Kielczewska
Agnieszka
A
Department of Human Genetics, McGill University, Montreal, Quebec, Canada.
Kim
Hee-Seo
HS
Lanier
Lewis L
LL
Dimasi
Nazzareno
N
Vidal
Silvia M
SM
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D000950:Antigens, Ly / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D019281:Dimerization; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology*; D037181:Lectins, C-Type / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008958:Models, Molecular; D008969:Molecular Sequence Data; D018146:Muromegalovirus / Q000276:immunology*; D016296:Mutagenesis; D055650:NK Cell Lectin-Like Receptor Subfamily A; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D055630:Receptors, NK Cell Lectin-Like
D000950:Antigens, Ly; D000956:Antigens, Viral; D015395:Histocompatibility Antigens Class I; C527091:Klra3 protein, mouse; C527096:Klra8 protein, mouse; D037181:Lectins, C-Type; D055650:NK Cell Lectin-Like Receptor Subfamily A; D055630:Receptors, NK Cell Lectin-Like