Protection of shrimp (Penaeus chinensis) against white spot syndrome virus (WSSV) challenge by double-stranded RNA.
To determine whether Penaeus chinensis can be protected against white spot syndrome virus (WSSV) infection by intramuscular injection with long double-stranded RNAs (dsRNAs) as in other shrimp species and whether the protection degree by WSSV-specific dsRNAs is correlated with the roles of viral genes, P. chinensis juveniles were intramuscularly injected with long dsRNAs corresponding to VP28, VP281, protein kinase genes of WSSV, and an unrelated long dsRNA corresponding to a green fluorescence protein (GFP) gene. All shrimp injected with long dsRNAs including GFP dsRNA showed higher survival rates against WSSV infection than shrimp injected with PBS alone. Furthermore, shrimp injected with dsRNAs corresponding to VP28 and protein kinase showed higher survival rates than those injected with dsRNAs corresponding to VP281 and GFP. These results indicate that the introduction of long dsRNAs corresponding to viral proteins, which are essential for WSSV infection, is quite effective in blocking WSSV infection in P. chinensis, and suggest that dsRNA-mediated protection is a common feature across shrimp species.
Kim
Chun Soo
CS
Department of Aquatic Life Medicine, Pukyong National University, 599-1 Daeyeondong, Namgu, Pusan 608-737, South Korea.
Kosuke
Zenke
Z
Nam
Yoon Kwon
YK
Kim
Sung Koo
SK
Kim
Ki Hong
KH
D000818:Animals; D017931:DNA Primers; D005814:Genes, Viral / Q000276:immunology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D033561:Penaeidae / Q000276:immunology* / Q000821:virology; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000276:immunology*; D016019:Survival Analysis; D046848:White spot syndrome virus 1 / Q000276:immunology*
D017931:DNA Primers; D012330:RNA, Double-Stranded; D049452:Green Fluorescent Proteins