title: PEG-induced fusion of phosphatidylcholine-liposomes with protoplasts and post-fusion evaluation of plating efficiency and enrichment in plasmamembrane phosphatidylcholine of protoplasts in Datura innoxia Mill.
abstract: Liposomes entrapping fluorescein diacetate were fused with protoplasts of Datura innoxia Mill by employing polyethylene glycol (PEG) as the fusogen. Factors that influence liposome-protoplast fusion were optimized as a function of PEG-concentration and incubation duration, liposome composition and surface charge and liposome:protoplast ratio. Phosphatidylcholine-liposomes were found ideal for the objectives of the study. Fusion index based on per cent fluorescing protoplasts varied among the protoplast types. PEG-incubation duration in the fusion assay and growth ability of protoplasts to form microcalli subsequent to liposome-protoplast fusion was determined based on protoplast plating-efficiency. Plating efficiency of post-fusion protoplasts increased due to incorporation of liposome-phosphatidylcholine in the plasmamembrane of protoplasts. Results are discussed in relation to the application of liposome-protoplast fusion system in selective modification of plasmamembrane phospholipids of protoplasts.
authors.affiliation: Department of Plant Sciences, School of Biological Sciences, Madurai Kamaraj University, Madurai 625 021, India. manohara2000@yahoo.com
authors.forename: Kumariah
authors.initials: K
authors.lastname: Manoharan
authors.forename: Rajendra
authors.initials: R
authors.lastname: Prasad
authors.forename: Sipra
authors.initials: S
authors.lastname: Guha-Mukherjee
mesh_terms: D002462:Cell Membrane / Q000737:chemistry; D032302:Datura / Q000737:chemistry* / Q000648:ultrastructure; D008081:Liposomes; D010713:Phosphatidylcholines / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011523:Protoplasts / Q000737:chemistry*
chemical_list: D008081:Liposomes; D010713:Phosphatidylcholines; D011092:Polyethylene Glycols