title: Production of siRNA- and cDNA-transfected cell arrays on noncoated chambered coverglass for high-content screening microscopy in living cells.
abstract: In this chapter, we provide a protocol for the production of transfected cell arrays in living mammalian cells on noncoated chambered coverglass for the systematic functional analyses of human genes by high-content screening microscopy. This method should facilitate drug target validation by small-interfering RNAs.
authors.affiliation: Cell Biology and Cell Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: Holger
authors.initials: H
authors.lastname: Erfle
authors.forename: Rainer
authors.initials: R
authors.lastname: Pepperkok
mesh_terms: D007091:Image Processing, Computer-Assisted; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D034741:RNA, Small Interfering; D013499:Surface Properties; D014162:Transfection
chemical_list: D034741:RNA, Small Interfering