Strain-typing of Lentinula edodes in China with inter simple sequence repeat markers.
To validate strain typing by inter simple sequence repeat (ISSR) analysis in Lentinula edodes cultivars, 17 Chinese L. edodes strains including 15 cultivated strains cultivated on a large scale and two wild strains were analyzed with the ISSR technique. With the use of two ISSR primers, a total of 32 DNA products were detected, of which, 31 DNA products (96.9% of the detected products) were polymorphic between two or more strains. The profiles of those two primers could be employed to differentiate all of the tested strains. A cluster analysis based on ISSR data revealed that the 17 strains could be classified into two distinct groups. One group consisted of eight strains in which the cultivated strains were H (high-temperature)-type or B (broad-temperature)-type, and the other group comprised cultivated strains that were of the L (low-temperature)-type or M (medium-temperature)-type. In contrast to the two wild strains, the genetic diversity of 15 cultivated strains was very rich based on a similarity coefficient analysis.
Zhang
Ruiying
R
Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Huang
Chenyang
C
Zheng
Suyue
S
Zhang
Jinxia
J
Ng
Tzi Bun
TB
Jiang
Ruibo
R
Zuo
Xuemei
X
Wang
Hexiang
H
D002681:China; D004271:DNA, Fungal / Q000032:analysis / Q000302:isolation & purification; D005819:Genetic Markers; D014644:Genetic Variation; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D020942:Shiitake Mushrooms / Q000145:classification* / Q000235:genetics* / Q000254:growth & development; D013045:Species Specificity
D004271:DNA, Fungal; D005819:Genetic Markers