Recent developments and future prospects of Vitreoscilla hemoglobin application in metabolic engineering.
In hypoxic conditions, bacteria express a kind of hemoglobin, which is proposed to enhance respiration and energy metabolism by promoting oxygen delivery. Bacteria hemoglobin from Vitreoscilla stercoraria - Vitreoscilla hemoglobin (VHb), when expressed in various hosts in oxygen-limited conditions, has been shown to improve growth, protein secretion, metabolite productivity and stress resistance of hosts, thus rendering the protein promising in metabolic engineering, especially in plant metabolism optimization. In this review, many well-studies areas are presented to illustrate the potential of VHb application in biotechnology industry, to discuss the cellular mechanisms of VHb function and to show the wide variety of approaches taken within the field.
Zhang
Lei
L
State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R and D Center, Fudan University, Shanghai 200433, PR China.
Li
Yingjun
Y
Wang
Zinan
Z
Xia
Yang
Y
Chen
Wansheng
W
Tang
Kexuan
K
D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D035264:Flowers; D005818:Genetic Engineering / Q000379:methods / Q000639:trends*; D006454:Hemoglobins / Q000235:genetics* / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods* / Q000639:trends; D054793:Truncated Hemoglobins; D020589:Vitreoscilla
D001426:Bacterial Proteins; D006454:Hemoglobins; D054793:Truncated Hemoglobins; C082613:hemoglobin protein, Vitreoscilla; D010100:Oxygen