Open-access microfluidic patch-clamp array with raised lateral cell trapping sites.
A novel open-access microfluidic patch-clamp array chip with lateral cell trapping sites raised above the bottom plane of the chip was developed by combining both a microscale soft-lithography and a macroscale polymer fabrication method. This paper demonstrates the capability of using such an open-access fluidic system for patch-clamp measurements. The surface of the open-access patch-clamp sites prepared by the macroscale hole patterning method of soft-state elastic polydimethylsiloxane (PDMS) is examined; the seal resistances are characterized and correlated with the aperture dimensions. Whole cell patch-clamp measurements are carried out with CHO cells expressing Kv2.1 ion channels. Kv2.1 ion channel blocker (TEA) dosage response is characterized and the binding activity is examined. The results demonstrate that the system is capable of performing whole cell measurements and drug profiling in a more efficient manner than the traditional patch-clamp set-up.
Lau
Adrian Y
AY
Biomolecular Nanotechnology Center, Berkeley Sensor & Actuator Center, Department of Bioengineering, University of California-Berkeley, Berkeley, CA 94720, USA.
Hung
Paul J
PJ
Wu
Angela R
AR
Lee
Luke P
LP
D000818:Animals; D016466:CHO Cells; D002478:Cells, Cultured; D006224:Cricetinae; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006367:HeLa Cells; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods; D018408:Patch-Clamp Techniques / Q000295:instrumentation / Q000379:methods; D016062:Porosity; D026902:Potassium Channel Blockers / Q000494:pharmacology; D024642:Potassium Channels, Voltage-Gated / Q000187:drug effects; D012680:Sensitivity and Specificity; D012825:Silicon / Q000737:chemistry; D012828:Silicones / Q000737:chemistry; D013499:Surface Properties; D019789:Tetraethylammonium / Q000494:pharmacology
D004129:Dimethylpolysiloxanes; D026902:Potassium Channel Blockers; D024642:Potassium Channels, Voltage-Gated; D012828:Silicones; C013830:baysilon; D019789:Tetraethylammonium; D012825:Silicon