title: Materials specificity and directed assembly of a gold-binding peptide.
abstract: Adsorption studies of a genetically engineered gold-binding peptide, GBP1, were carried out using a quartz-crystal microbalance (QCM) to quantify its molecular affinity to noble metals. The peptide showed higher adsorption onto and lower desorption from a gold surface compared to a platinum substrate. The material specificity, that is, the preferential adsorption, of GBP1 was also demonstrated using gold and platinum micropatterned on a silicon wafer containing native oxide. The biotinylated three-repeat units of GBP1 were preferentially adsorbed onto gold regions delineated using streptavidin-conjugated quantum dots (SAQDs). These experiments not only demonstrate that an inorganic-binding peptide could preferentially adsorb onto a metal (Au) rather than an oxide (SiO2) but also onto one noble metal (Au) over another (Pt). This result shows the utility of an engineered peptide as a molecular erector in the directed immobilization of a nanoscale hybrid entity (SAQDs) over selected regions (Au) on a fairly complex substrate (Au and Pt micropatterned regions on silica). The selective and controlled adsorption of inorganic-binding peptides may have significant implications in nano- and nanobiotechnology, where they could be genetically tailored for specific use in the development of self-assembled molecular systems.
authors.affiliation: Materials Science & Engineering Department, Roberts Hall, Box: 352120, University of Washington, Seattle, WA 98195, USA.
authors.forename: Candan
authors.initials: C
authors.lastname: Tamerler
authors.forename: Memed
authors.initials: M
authors.lastname: Duman
authors.forename: Ersin Emre
authors.initials: EE
authors.lastname: Oren
authors.forename: Mustafa
authors.initials: M
authors.lastname: Gungormus
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Xiong
authors.forename: Turgay
authors.initials: T
authors.lastname: Kacar
authors.forename: Babak A
authors.initials: BA
authors.lastname: Parviz
authors.forename: Mehmet
authors.initials: M
authors.lastname: Sarikaya
mesh_terms: D000327:Adsorption; D019921:Biotinylation; D004305:Dose-Response Relationship, Drug; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D007700:Kinetics; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D036103:Nanotechnology / Q000379:methods*; D010455:Peptides / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry; D015202:Protein Engineering / Q000379:methods*; D045663:Quantum Dots; D013816:Thermodynamics
chemical_list: D010455:Peptides; D010984:Platinum; D006046:Gold