A BAC-based contig map of the cynomolgus macaque (Macaca fascicularis) major histocompatibility complex genomic region.
The construction of a cynomolgus macaque (Macaca fascicularis, Mafa) BAC library for genomic comparison between rhesus and cynomolgus macaques is necessary to promote the cynomolgus macaque as one of the important experimental animals for future medical and biological research. In this paper, we constructed a cynomolgus macaque BAC library and a map of the MHC (Mafa) genomic region for comparison of the genomic organization and nucleotide similarities between the human, the chimpanzee, and the rhesus macaque. The BAC library consists of 221,184 clones with an average insert size of 83 kb, providing a sixfold coverage of the haploid genome. A total of 114 BAC clones and 54 PCR primer sets were used to construct a 4.3-Mb contig of the MHC region. Diversity analysis of genomic sequence from selected subregions of the MHC revealed that the cynomolgus sequence varied compared to rhesus macaque, human, and chimpanzee sequences by 0.48, 4.15, and 4.10%, respectively. From these findings, we conclude that the BAC library and Mafa genomic map are useful tools for genome analysis and will have important applications for comparative genomics and identifying regions of consequence in medical research.
Watanabe
Atsushi
A
Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa 259-1143, Japan.
Shiina
Takashi
T
Shimizu
Sayoko
S
Hosomichi
Kazuyoshi
K
Yanagiya
Kazuyo
K
Kita
Yuki F
YF
Kimura
Tetsuaki
T
Soeda
Eiichi
E
Torii
Ryuzo
R
Ogasawara
Kazumasa
K
Kulski
Jerzy K
JK
Inoko
Hidetoshi
H
D000818:Animals; D022202:Chromosomes, Artificial, Bacterial; D020451:Contig Mapping; D015723:Gene Library; D005805:Genes, MHC Class I; D005802:Genes, MHC Class II; D006801:Humans; D008252:Macaca fascicularis / Q000235:genetics*; D008285:Major Histocompatibility Complex / Q000235:genetics*; D002679:Pan troglodytes / Q000235:genetics; D010802:Phylogeny; D016415:Sequence Alignment