[Nanoelectrochemistry of cytochrome P450s: direct electron transfer and electrocatalysis].
The present study demonstrates the direct electron transfer between cytochrome P450 2B4 (CYP2B4), P450 1A2 (CYP1A2), sterol 14alpha-demethylase (CYP51MT) and screen printed graphite electrodes, modified with gold nanoparticles and didodecyldimethylammonium bromide (DDAB). Electrodetection of heme proteins is possible when 2-200 pmol P450/electrode were adsorbed on the surface of nanostructured electrochemical interfaces. Electron transfer, direct electrochemical reduction and interaction with P450 substrates (oxygen, benzphetamine, lanosterol) and inhibitor ketoconazole were analyzed using cyclic voltammetry (CV), square wave (SWV) or differential pulse (DPV) voltammetry, amperometry.
Shumiantseva
V V
VV
Bulko
T V
TV
Rudakov
Iu O
IuO
Samenkova
N F
NF
Lisitsa
A V
AV
Karuzina
I I
II
Archakov
A I
AI
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000737:chemistry*; D019388:Cytochrome P-450 CYP1A2 / Q000737:chemistry*; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry*; D000072467:Cytochrome P450 Family 2; D004563:Electrochemistry / Q000379:methods; D004579:Electron Transport; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008839:Microelectrodes; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D011817:Rabbits; D058886:Sterol 14-Demethylase
D000644:Quaternary Ammonium Compounds; C046112:didodecyldimethylammonium; D006046:Gold; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D001189:Aryl Hydrocarbon Hydroxylases; D019388:Cytochrome P-450 CYP1A2; D000072467:Cytochrome P450 Family 2; C465241:cytochrome P-450 CYP2B4 (rabbit); D058886:Sterol 14-Demethylase