Alternative splicing generates a novel non-secretable cytosolic isoform of NELL2.
NELL2 is as a neuron-specific secreted glycoprotein. The present study provides evidence of an alternatively spliced variant of the rat NELL2 gene that yields cytosolic NELL2 (cNELL2). cNELL2 was initially detected in the thymus and subsequently found to be ubiquitously expressed in many other tissues. The absence of the sequences corresponding to the third exon, which contains the terminal portion of the signal peptide, accounts for the uniform distribution of cNELL2 throughout the cytoplasm. This is in contrast to NELL2, which is preferentially located at distinct subcellular structures involved in the secretary process, such as endoplasmic reticulum and Golgi apparatus. Western blot analysis showed that cNELL2 was not present in the medium but only in lysates, while NELL2 was detected as a glycosylated larger form in both lysates and media. Immunoprecipitation analysis revealed that cNELL2 interacts with PKCbeta1. These results suggest that cNELL2 is involved in PKCbeta1-mediated intracellular signaling.
Hwang
Eun Mi
EM
Department of Neurobiology, Institute of Health Science, and Medical Research Center for Neural Dysfunction, Gyeongsang National University College of Medicine, 90 Chilam-Dong, Jinju 660-751, Republic of Korea.
Kim
Dong-Gyu
DG
Lee
Byung Ju
BJ
Choi
Jungil
J
Kim
Eunju
E
Park
Nammi
N
Kang
Dawon
D
Han
Jaehee
J
Choi
Wan Sung
WS
Hong
Seong-Geun
SG
Park
Jae-Yong
JY
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003600:Cytosol / Q000378:metabolism*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism; D064546:Protein Kinase C beta; D051381:Rats
C491252:Nell2 protein, rat; D009419:Nerve Tissue Proteins; D011493:Protein Kinase C; D064546:Protein Kinase C beta