title: Heat shock response and mammal adaptation to high elevation (hypoxia).
abstract: The mammal's high elevation (hypoxia) adaptation was studied by using the immunological and the molecular biological methods to understand the significance of Hsp (hypoxia) adaptation in the organic high elevation, through the mammal heat shock response. (1) From high elevation to low elevation (natural hypoxia): Western blot and conventional RT-PCR and real-time fluorescence quota PCR were adopted. Expression difference of heat shock protein of 70 (Hsp70) and natural expression of brain tissue of Hsp70 gene was determined in the cardiac muscle tissue among the different elevation mammals (yak). (2) From low elevation to high elevation (hypoxia induction): The mammals (domestic rabbits) from the low elevation were sent directly to the areas with different high elevations like 2300, 3300 and 5000 m above sea level to be raised for a period of 3 weeks before being slaughtered and the genetic inductive expression of the brain tissue of Hsp70 was determined with RT-PCR. The result indicated that all of the mammals at different elevations possessed their heat shock response gene. Hsp70 of the high elevation mammal rose abruptly under stress and might be induced to come into being by high elevation (hypoxia). The speedy synthesis of Hsp70 in the process of heat shock response is suitable to maintain the cells' normal physiological functions under stress. The Hsp70 has its threshold value. The altitude of 5000 m above sea level is the best condition for the heat shock response, and it starts to reduce when the altitude is over 6000 m above sea level. The Hsp70 production quantity and the cell hypoxia bearing capacity have their direct ratio.
authors.affiliation: Northwest Plateau Biological Research Institute, Chinese Academy of Sciences, Xining 810001, China. Wangxl63@126.com
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Wang
authors.forename: Cunshuan
authors.initials: C
authors.lastname: Xu
authors.forename: Xiujie
authors.initials: X
authors.lastname: Wang
authors.forename: Dongjie
authors.initials: D
authors.lastname: Wang
authors.forename: Qingshang
authors.initials: Q
authors.lastname: Wang
authors.forename: Baochen
authors.initials: B
authors.lastname: Zhang
mesh_terms: D000064:Acclimatization / Q000235:genetics / Q000502:physiology*; D000531:Altitude; D000818:Animals; D002417:Cattle / Q000235:genetics / Q000502:physiology*; D005786:Gene Expression Regulation; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D018869:Heat-Shock Response / Q000235:genetics / Q000502:physiology*; D000860:Hypoxia / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits
chemical_list: D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger