title: Functional interaction between PML and SATB1 regulates chromatin-loop architecture and transcription of the MHC class I locus.
abstract: The function of the subnuclear structure the promyelocytic leukaemia (PML) body is unclear largely because of the functional heterogeneity of its constituents. Here, we provide the evidence for a direct link between PML, higher-order chromatin organization and gene regulation. We show that PML physically and functionally interacts with the matrix attachment region (MAR)-binding protein, special AT-rich sequence binding protein 1 (SATB1) to organize the major histocompatibility complex (MHC) class I locus into distinct higher-order chromatin-loop structures. Interferon gamma (IFNgamma) treatment and silencing of either SATB1 or PML dynamically alter chromatin architecture, thus affecting the expression profile of a subset of MHC class I genes. Our studies identify PML and SATB1 as a regulatory complex that governs transcription by orchestrating dynamic chromatin-loop architecture.
authors.affiliation: National Centre for Cell Science, Ganeshkhind, Pune 411007, India.
authors.forename: Pavan P
authors.initials: PP
authors.lastname: Kumar
authors.forename: Oliver
authors.initials: O
authors.lastname: Bischof
authors.forename: Prabhat Kumar
authors.initials: PK
authors.lastname: Purbey
authors.forename: Dimple
authors.initials: D
authors.lastname: Notani
authors.forename: Henning
authors.initials: H
authors.lastname: Urlaub
authors.forename: Anne
authors.initials: A
authors.lastname: Dejean
authors.forename: Sanjeev
authors.initials: S
authors.lastname: Galande
mesh_terms: D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000737:chemistry / Q000235:genetics*; D005786:Gene Expression Regulation; D005805:Genes, MHC Class I; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics* / Q000378:metabolism; D036961:Matrix Attachment Region Binding Proteins / Q000235:genetics* / Q000378:metabolism; D045170:Matrix Attachment Regions / Q000235:genetics; D008958:Models, Molecular; D020033:Protein Isoforms; D034622:RNA Interference; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D002843:Chromatin; D036961:Matrix Attachment Region Binding Proteins; D020033:Protein Isoforms; C076189:SATB1 protein, human; D007371:Interferon-gamma