title: Effect of exogenous calcium on post-thaw growth recovery and subsequent plant regeneration of cryopreserved embryogenic calli of Hevea brasiliensis (Müll. Arg.).
abstract: A reliable cryopreservation technique was developed for friable embryogenic callus lines of Hevea brasiliensis. The study showed that reducing the CaCl(2) concentration of the pre-culture medium from 9 mM to 1 or 0 mM CaCl(2) before cryopreservation promoted post-thaw callus growth, 1 mM being the optimum CaCl(2) concentration for embryo regeneration. Post-thaw callus proliferation decreased in line with the increase of plated callus weight. The effect of cryopreservation was assessed on 39 independent lines showing that cryopreservation did not affect embryogenic and plant regeneration for a majority of lines. The decrease in CaCl(2) concentration of the pre-culture medium led to a drop in callus calcium content indicating a direct link between the CaCl(2) concentration of the pre-culture medium and the endogenous calcium content of the calli. It also highlighted the implication of tissue calcium content in cryotolerance. Callus water status and the different ways by which calcium could prevent cryoinjury is also discussed.
authors.affiliation: CIRAD-CP, UMR 1098, TA 80/03, Avenue Agropolis, 34398 Montpellier, France. ludovic.lardet@cirad.fr
authors.forename: Ludovic
authors.initials: L
authors.lastname: Lardet
authors.forename: Florence
authors.initials: F
authors.lastname: Martin
authors.forename: Florence
authors.initials: F
authors.lastname: Dessailly
authors.forename: Marc-Philippe
authors.initials: MP
authors.lastname: Carron
authors.forename: Pascal
authors.initials: P
authors.lastname: Montoro
mesh_terms: D002122:Calcium Chloride / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D015925:Cryopreservation; D003470:Culture Media; D047108:Embryonic Development / Q000187:drug effects*; D028482:Hevea / Q000187:drug effects / Q000196:embryology / Q000254:growth & development* / Q000502:physiology*; D009929:Organ Size / Q000187:drug effects; D012038:Regeneration / Q000187:drug effects*; D014867:Water / Q000378:metabolism
chemical_list: D003470:Culture Media; D014867:Water; D002122:Calcium Chloride