title: Study on interaction between microtubule associated protein tau and prion protein.
abstract: Microtubule-associated protein tau is considered to play roles in many neurodegenerative diseases including some transmissible spongiform encephalopathies. To address the possible molecular linkage of prion protein (PrP) and tau, a GST-fusion segment of human tau covering the three-repeat region and various PrP segments was used in the tests of GST pull-down and immunoprecipitation. We found tau protein interacted with various style prion proteins such as native prion protein (PrPc) or protease-resistant isoform (PrPSc). Co-localization signals of tau and PrP were found in the CHO cell tranfected with both PrP and tau gene. The domain of interaction with tau was located at N-terminal of PrP (residues 23 to 91). The evidence of molecular interactions between PrP and tau protein highlights a potential role of tau in the biological function of PrP and the pathogenesis of TSEs.
authors.affiliation: State Key Laboratory for Infectious Diseases Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Han
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.forename: Hailan
authors.initials: H
authors.lastname: Yao
authors.forename: Xiaofan
authors.initials: X
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Lan
authors.initials: L
authors.lastname: Chen
authors.forename: Chen
authors.initials: C
authors.lastname: Gao
authors.forename: Jianmei
authors.initials: J
authors.lastname: Gao
authors.forename: Kai
authors.initials: K
authors.lastname: Nie
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Dong
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D002460:Cell Line; D015870:Gene Expression; D006801:Humans; D008870:Microtubules / Q000378:metabolism*; D011328:Prions / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016875:tau Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D011328:Prions; D011994:Recombinant Proteins; D016875:tau Proteins