title: Identification of a new Schistosoma mansoni membrane-bound protein through bioinformatic analysis.
abstract: Progress in schistosome genome research has enabled investigators to move rapidly from genome sequences to vaccine development. Proteins bound to the surface of parasites are potential vaccine candidates, or they can be used for diagnosis. We analyzed 4342 proteins deduced from the Schistosoma mansoni transcriptome with bioinformatic computer programs. Thirty-four proteins had membrane-bound motifs. Within this group, we selected the Sm29 protein to be further characterized by in silico analysis. Sm29 was found to have a signal peptide made up of 26 amino acids, with a cleavage site between Ser26 and Val27. The glycosylation site search revealed three threonines (39, 132 and 133) with high probability of O-glycosylation and two asparagines (58 and 115) with high probability of N-glycosylation. Only one transmembrane helix was found in the C-terminal region of the protein from Leu169 to Lis191. The search for similarities and conserved motifs show that Sm29 is a protein with high identity to proteins present in S. japonicum (53, 52, 49, and 37% of identity) and it possesses disulfide-rich conserved domains. Apparently, Sm29 is a membrane bound protein, and it may be an important molecule in host-parasite interactions.
authors.affiliation: Departamento de Bioquímica e Imunologia, Instituto de Investigação em Imunologia, Instituto do Milênio, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
authors.forename: F C
authors.initials: FC
authors.lastname: Cardoso
authors.forename: J M R
authors.initials: JM
authors.lastname: Pinho
authors.forename: V
authors.initials: V
authors.lastname: Azevedo
authors.forename: S C
authors.initials: SC
authors.lastname: Oliveira
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019295:Computational Biology; D023281:Genomics; D015801:Helminth Proteins / Q000235:genetics / Q000302:isolation & purification*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D012550:Schistosoma mansoni / Q000737:chemistry / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D015801:Helminth Proteins; D008562:Membrane Glycoproteins