[Direct sequencing and analysis of exons of Wilson' disease gene with the most mutations: a study in Hunan Han patients].
OBJECTIVE
To detect hot point mutations of ATP7B gene in Hunan Han patients with Wilson' disease (WD).


METHODS
The genomic DNA of 22 WD patients was extracted and exons 5, 8, 12, 13 were amplified by PCR. Screening for the mutations was done by direct sequencing and analysed by BLAST.


RESULTS
Fifteen of the 22 patients were found with mutations. Ten heterozygous Arg778Leu (2273G --> T) mutations were found in exon 8, all of them were accompanied with 2250C --> G polymorphism (Leu770Leu). Seven patients were found with 2855G --> A (Arg952Lys) polymorphism (4 heterozygous and 3 homozygous), 3 of them had Arg778Leu mutation in exon 8 and one with heterozygous mutation Gly943Asp (2828G --> A) in exon 12 simultaneously. Only one patient was found with heterozygous Pro992Leu (2975C --> T) mutation in exon 13. No mutations were found in exon 5.


CONCLUSIONS
Arg778Leu is the hot point mutation of ATP7B gene in Hunan Han patients with Wilson' disease while exon 5 is not.
Luo
Kai-Zhong
KZ
Research Center for Liver Diseases, Second Xiangya Hospital, Central South University, Changsha 410011, China. luokz@hotmail.com
Yang
Xu
X
Tong
De-Jun
DJ
He
Gang
G
Yi
Lu-Xi
LX
D000251:Adenosine Triphosphatases / Q000235:genetics*; D000293:Adolescent; D044466:Asian People / Q000235:genetics; D027682:Cation Transport Proteins / Q000235:genetics*; D002648:Child; D000073840:Copper-Transporting ATPases; D004247:DNA / Q000235:genetics; D004252:DNA Mutational Analysis; D005091:Exons; D006527:Hepatolenticular Degeneration / Q000208:ethnology / Q000235:genetics*; D006801:Humans; D009154:Mutation
D027682:Cation Transport Proteins; D004247:DNA; D000251:Adenosine Triphosphatases; C000612464:ATP7B protein, human; D000073840:Copper-Transporting ATPases