Real-time monitoring for bioaerosols--flow cytometry.
Bioaerosol detection in real time is an urgent civilian and military requirement. In this article, bioaerosol mass spectrometry, an instrument for real-time detection of bioaerosols using simultaneous measurement of particle aerodynamic size and intrinsic fluorescence, real-time qPCR, and FCM/FL were discussed. Although, challenging work remains to determine the interfering substances (e.g. particulates) of different environments, distinguish the specific species with specific probe, and overcome the high detection limit of FCM (10(4)-10(8) cells ml(-1)), literature reports suggested that FCM/FL has a great potential for real-time monitoring of bioaerosols.
Chen
Pei-Shih
PS
Department of Public Health, Kaohsiung Medical University, Kaohsiung, Taiwan.
Li
Chih-Shan
CS
D000336:Aerosols; D000391:Air Microbiology; D000397:Air Pollution / Q000032:analysis*; D001694:Biological Warfare; D005434:Flow Cytometry; D013058:Mass Spectrometry; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000336:Aerosols