title: Sensitive protein detection via triple-binder proximity ligation assays.
abstract: The detection of weakly expressed proteins and protein complexes in biological samples represents a fundamental challenge. We have developed a new proximity-ligation strategy named 3PLA that uses three recognition events for the highly specific and sensitive detection of as little as a hundred molecules of the vascular endothelial growth factor (VEGF), the biomarkers troponin I, and prostate-specific antigen (PSA) alone or in complex with an inhibitor--demonstrating the versatility of 3PLA.
authors.affiliation: Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Se-75185 Uppsala, Sweden. edith.schallmeiner@genpat.uu.se
authors.forename: Edith
authors.initials: E
authors.lastname: Schallmeiner
authors.forename: Elli
authors.initials: E
authors.lastname: Oksanen
authors.forename: Olle
authors.initials: O
authors.lastname: Ericsson
authors.forename: Lena
authors.initials: L
authors.lastname: Spångberg
authors.forename: Susann
authors.initials: S
authors.lastname: Eriksson
authors.forename: Ulf-Håkan
authors.initials: UH
authors.lastname: Stenman
authors.forename: Kim
authors.initials: K
authors.lastname: Pettersson
authors.forename: Ulf
authors.initials: U
authors.lastname: Landegren
mesh_terms: D000906:Antibodies / Q000378:metabolism; D001710:Biotin / Q000378:metabolism; D006801:Humans; D007118:Immunoassay; D015336:Molecular Probe Techniques; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000378:metabolism; D016133:Polymerase Chain Reaction; D017430:Prostate-Specific Antigen / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D012680:Sensitivity and Specificity; D019809:Streptavidin / Q000378:metabolism; D019210:Troponin I / Q000032:analysis; D020298:U937 Cells; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis
chemical_list: D000906:Antibodies; D009841:Oligonucleotides; D011506:Proteins; D019210:Troponin I; D042461:Vascular Endothelial Growth Factor A; D001710:Biotin; D019809:Streptavidin; D017430:Prostate-Specific Antigen