Talosins A and B: new isoflavonol glycosides with potent antifungal activity from Kitasatospora kifunensis MJM341. I. Taxonomy, fermentation, isolation, and biological activities.
In our screening program for new antifungal agents from microbial secondary metabolites, we isolated two new isoflavonol glycosides, genistein 7-alpha-L-6-deoxy-talopyranoside (talosin A) and genistein 4',7-di-alpha-L-6-deoxy-talopyranoside (talosin B), from the culture broth of Kitasatospora kifunensis MJM341. The talosins exhibited strong antifungal activity against Candida albicans, Aspergillus niger and Cryptococcus neoformans with minimal inhibitory concentrations (MIC) in the range of 3- 15 microg/ml while genistein and genistein-7-glucopyranoside did not show antifungal activity at 100 microg/ml. These talosins are the first isoflavonol glycosides with a 6-deoxy-talose sugar component and they may be useful as antifungal agents with low toxicity because of no visible cytotoxicity against the human hepatic HepG2 cell.
Yoon
Tae Mi
TM
Department of Biological Science, Institute of Bioscience and Biotechnology, Myongji University, Yongin, Gyeonggi-Do, 449-728, Korea.
Kim
Jong Woo
JW
Kim
Jong Gwan
JG
Kim
Won Gon
WG
Suh
Joo Won
JW
D000935:Antifungal Agents / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D001234:Aspergillus niger / Q000187:drug effects; D002176:Candida albicans / Q000187:drug effects; D002965:Classification; D003455:Cryptococcus neoformans / Q000187:drug effects; D005285:Fermentation; D006027:Glycosides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D007529:Isoflavones / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D015394:Molecular Structure; D013306:Streptomycetaceae / Q000737:chemistry* / Q000145:classification* / Q000378:metabolism
D000935:Antifungal Agents; D006027:Glycosides; D007529:Isoflavones; C516692:genistein 4',7'-bis(6-deoxytalopyranoside); C516691:genistein 7-(6-deoxytalopyranoside)