title: Lung cancer induced in mice by the envelope protein of jaagsiekte sheep retrovirus (JSRV) closely resembles lung cancer in sheep infected with JSRV.
abstract: BACKGROUND
  Jaagsiekte sheep retrovirus (JSRV) causes a lethal lung cancer in sheep and goats. Expression of the JSRV envelope (Env) protein in mouse lung, by using a replication-defective adeno-associated virus type 6 (AAV6) vector, induces tumors resembling those seen in sheep. However, the mouse and sheep tumors have not been carefully compared to determine if Env expression alone in mice can account for the disease features observed in sheep, or whether additional aspects of virus replication in sheep are important, such as oncogene activation following retrovirus integration into the host cell genome.
  
  
  RESULTS
  We have generated mouse monoclonal antibodies (Mab) against JSRV Env and have used these to study mouse and sheep lung tumor histology. These Mab detect Env expression in tumors in sheep infected with JSRV from around the world with high sensitivity and specificity. Mouse and sheep tumors consisted mainly of well-differentiated adenomatous foci with little histological evidence of anaplasia, but at long times after vector exposure some mouse tumors did have a more malignant appearance typical of adenocarcinoma. In addition to epithelial cell tumors, lungs of three of 29 sheep examined contained fibroblastic cell masses that expressed Env and appeared to be separate neoplasms. The Mab also stained nasal adenocarcinoma tissue from one United States sheep, which we show was due to expression of Env from ovine enzootic nasal tumor virus (ENTV), a virus closely related to JSRV. Systemic administration of the AAV6 vector encoding JSRV Env to mice produced numerous hepatocellular tumors, and some hemangiomas and hemangiosarcomas, showing that the Env protein can induce tumors in multiple cell types.
  
  
  CONCLUSIONS
  Lung cancers induced by JSRV infection in sheep and by JSRV Env expression in mice have similar histologic features and are primarily characterized by adenomatous proliferation of peripheral lung epithelial cells. Thus it is unnecessary to invoke a role for insertional mutagenesis, gene activation, viral replication, or expression of other viral gene products in sheep lung tumorigenesis, although these processes may play a role in other clinically less important sequelae of JSRV infection such as metastasis observed with variable frequency in sheep.
authors.affiliation: Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA. swootton@fhcrc.org
authors.forename: Sarah K
authors.initials: SK
authors.lastname: Wootton
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Metzger
authors.forename: Kelly L
authors.initials: KL
authors.lastname: Hudkins
authors.forename: Charles E
authors.initials: CE
authors.lastname: Alpers
authors.forename: Denis
authors.initials: D
authors.lastname: York
authors.forename: James C
authors.initials: JC
authors.lastname: DeMartini
authors.forename: A Dusty
authors.initials: AD
authors.lastname: Miller
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D000818:Animals; D015686:Gene Products, env / Q000378:metabolism*; D022141:Jaagsiekte sheep retrovirus / Q000378:metabolism / Q000472:pathogenicity*; D008168:Lung / Q000473:pathology / Q000821:virology; D008175:Lung Neoplasms / Q000473:pathology* / Q000821:virology; D051379:Mice; D008810:Mice, Inbred C57BL; D011648:Pulmonary Adenomatosis, Ovine / Q000473:pathology* / Q000821:virology; D012756:Sheep / Q000821:virology; D012757:Sheep Diseases / Q000473:pathology* / Q000821:virology
chemical_list: D015686:Gene Products, env