title: Reliable serodiagnosis of imported cystic echinococcosis with a commercial indirect hemagglutination assay.
abstract: A commercially available indirect hemagglutination assay (IHA) (Echinococcosis Fumouze; Laboratoires Fumouze, Levallois-Perret, France) was evaluated using sera from 52 patients with proven cystic echinococcosis. The specificity was assessed using 247 sera from patients with various parasitic, bacterial, viral, and fungal infectious diseases; sera containing autoimmune antibodies; and sera from healthy blood donors. With a cutoff value for a positive result of 320 (as recommended by the manufacturer), the sensitivity and specificity were 88% and 98.4%; with a cutoff of 160, the sensitivity and specificity were 94% and 95.1%, respectively. The IHA is rapid, easy to perform, and is a very sensitive serodiagnostic test for cystic echinococcosis.
authors.affiliation: Section of Parasitology, Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, PO Box 22660, 1100DD Amsterdam, The Netherlands. h.r.vandoorn@amc.uva.nl
authors.forename: H Rogier
authors.initials: HR
authors.lastname: van Doorn
authors.forename: Henk
authors.initials: H
authors.lastname: Hofwegen
authors.forename: Rob
authors.initials: R
authors.lastname: Koelewijn
authors.forename: Henk
authors.initials: H
authors.lastname: Gilis
authors.forename: Ellen
authors.initials: E
authors.lastname: Wentink-Bonnema
authors.forename: Elena
authors.initials: E
authors.lastname: Pinelli
authors.forename: Perry J J
authors.initials: PJ
authors.lastname: van Genderen
authors.forename: Hans G
authors.initials: HG
authors.lastname: Schipper
authors.forename: Tom
authors.initials: T
authors.lastname: van Gool
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000097:blood*; D004443:Echinococcosis / Q000175:diagnosis* / Q000469:parasitology; D004446:Echinococcus / Q000276:immunology*; D006386:Hemagglutination Tests / Q000379:methods*; D006801:Humans; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D000947:Antigens, Helminth; D011933:Reagent Kits, Diagnostic