Virus-induced gene silencing in plant roots.
Virus-induced gene silencing (VIGS) has been used as a quick and easy tool to assess gene function in plants. The virus, carrying a portion of an endogenous gene, triggers a homology-based defense mechanism when introduced into plants. Infection with the modified virus results in gene-specific transcript degradation. Several virus-based vectors have been developed for use in VIGS. One of these virus vectors, tobacco rattle virus (TRV), has a large host range and is transmitted by phytopathogenic nematodes of the genera Trichodorus and Paratrichodorus. TRV has been used in VIGS to study gene function in above-ground parts of plants. This chapter describes a protocol for the use of TRV vector to silence genes in roots.
Kaloshian
Isgouhi
I
Department of Nematology, University of California, Riverside, USA.
D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D020868:Gene Silencing; D017343:Genes, Plant / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D018551:Solanum lycopersicum / Q000235:genetics* / Q000382:microbiology / Q000821:virology*; D018517:Plant Roots / Q000235:genetics* / Q000382:microbiology / Q000821:virology*; D010942:Plant Viruses / Q000502:physiology*; D012231:Rhizobium / Q000502:physiology; D012639:Seeds / Q000821:virology