title: Ex vivo expansion of human cord blood hematopoietic progenitor cells using glutaraldehyde-fixed human bone marrow stromal cells.
abstract: Human stromal cells were immortalized and fixed with glutaraldehyde to support an ex vivo expansion of human cord blood hematopoietic progenitor cells. In addition, this enabled glutaraldehyde-fixed stromal cells to be stored at 4 degrees C. Although freeze-dried glutaraldehyde-fixed stromal cells did not increase the number of the progenitor cells, the percent decrease in the number of CD34(+) cells in the presence of freeze-dried glutaraldehyde-fixed stromal cells was less than that in the absence of the stromal cells. Thus, glutaraldehyde-fixed stromal cells can serve as a stabilizing device for hematopoietic cell expansion.
authors.affiliation: Regenerative Medical Bioreactor Project, Kanagawa Academy of Science and Technology, KSP East 309, 3-2-1 Sakado, Takatsu-ku, Kawasaki, Kanagawa 213-0012, Japan. y-ito@ksp.or.jp
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ito
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Hasauda
authors.forename: Takashi
authors.initials: T
authors.lastname: Kitajima
authors.forename: Toru
authors.initials: T
authors.lastname: Kiyono
mesh_terms: D018952:Antigens, CD34 / Q000032:analysis; D001854:Bone Marrow Cells / Q000502:physiology*; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology*; D005976:Glutaral / Q000494:pharmacology; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D017154:Stromal Cells / Q000502:physiology
chemical_list: D018952:Antigens, CD34; D005976:Glutaral