title: Complete genome sequence of Haemophilus somnus (Histophilus somni) strain 129Pt and comparison to Haemophilus ducreyi 35000HP and Haemophilus influenzae Rd.
abstract: Haemophilus somnus can be either a commensal of bovine mucosal surfaces or an opportunistic pathogen. Pathogenic strains of H. somnus are a significant cause of systemic disease in cattle. We report the genome sequence of H. somnus 129Pt, a nonpathogenic commensal preputial isolate, and the results of a genome-wide comparative analysis of H. somnus 129Pt, Haemophilus influenzae Rd, and Haemophilus ducreyi 35000HP. We found unique genes in H. somnus 129Pt involved in lipooligosaccharide biosynthesis, carbohydrate uptake and metabolism, cation transport, amino acid metabolism, ubiquinone and menaquinone biosynthesis, cell surface adhesion, biosynthesis of cofactors, energy metabolism, and electron transport. There were also many genes in common among the three organisms. Our comparative analyses of H. somnus 129Pt, H. influenzae Rd, and H. ducreyi 35000HP revealed similarities and differences in the numbers and compositions of genes involved in metabolism, host colonization, and persistence. These results lay a foundation for research on the host specificities and niche preferences of these organisms. Future comparisons between H. somnus 129Pt and virulent strains will aid in the development of protective strategies and vaccines to protect cattle against H. somnus disease.
authors.affiliation: Department of Energy Joint Genome Institute, Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA. jchalla@lanl.gov
authors.forename: Jean F
authors.initials: JF
authors.lastname: Challacombe
authors.forename: A J
authors.initials: AJ
authors.lastname: Duncan
authors.forename: Thomas S
authors.initials: TS
authors.lastname: Brettin
authors.forename: David
authors.initials: D
authors.lastname: Bruce
authors.forename: Olga
authors.initials: O
authors.lastname: Chertkov
authors.forename: J Chris
authors.initials: JC
authors.lastname: Detter
authors.forename: Cliff S
authors.initials: CS
authors.lastname: Han
authors.forename: Monica
authors.initials: M
authors.lastname: Misra
authors.forename: Paul
authors.initials: P
authors.lastname: Richardson
authors.forename: Roxanne
authors.initials: R
authors.lastname: Tapia
authors.forename: Nina
authors.initials: N
authors.lastname: Thayer
authors.forename: Gary
authors.initials: G
authors.lastname: Xie
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Inzana
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D001422:Bacterial Adhesion; D001483:Base Sequence; D002952:Citric Acid Cycle; D016680:Genome, Bacterial; D006191:Haemophilus ducreyi / Q000235:genetics* / Q000378:metabolism; D006193:Haemophilus influenzae / Q000235:genetics* / Q000378:metabolism; D044138:Haemophilus somnus / Q000235:genetics* / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism; D008969:Molecular Sequence Data; D009243:NAD / Q000096:biosynthesis; D009711:Nucleotides / Q000096:biosynthesis; D010427:Pentose Phosphate Pathway; D014168:Transferrin / Q000378:metabolism; D014451:Ubiquinone / Q000096:biosynthesis
chemical_list: D000596:Amino Acids; D006454:Hemoglobins; D009711:Nucleotides; D014168:Transferrin; D009243:NAD; D014451:Ubiquinone