Disruption of LDL but not VLDL clearance in autosomal recessive hypercholesterolemia.
Genetic defects in LDL clearance result in severe hypercholesterolemia and premature atherosclerosis. Mutations in the LDL receptor (LDLR) cause familial hypercholesterolemia (FH), the most severe form of genetic hypercholesterolemia. A phenocopy of FH, autosomal recessive hypercholesterolemia (ARH), is due to mutations in an adaptor protein involved in LDLR internalization. Despite comparable reductions in LDL clearance rates, plasma LDL levels are substantially lower in ARH than in FH. To determine the metabolic basis for this difference, we examined the synthesis and catabolism of VLDL in murine models of FH (Ldlr(-/-)) and ARH (Arh(-/-)). The hyperlipidemic response to a high-sucrose diet was greatly attenuated in Arh(-/-) mice compared with Ldlr(-/-) mice despite similar rates of VLDL secretion. The rate of VLDL clearance was significantly higher in Arh(-/-) mice than in Ldlr(-/-) mice, suggesting that LDLR-dependent uptake of VLDL is maintained in the absence of ARH. Consistent with these findings, hepatocytes from Arh(-/-) mice (but not Ldlr(-/-) mice) internalized beta-migrating VLDL (beta-VLDL). These results demonstrate that ARH is not required for LDLR-dependent uptake of VLDL by the liver. The preservation of VLDL remnant clearance attenuates the phenotype of ARH and likely contributes to greater responsiveness to statins in ARH compared with FH.
Jones
Christopher
C
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
Garuti
Rita
R
Michaely
Peter
P
Li
Wei-Ping
WP
Maeda
Nobuyo
N
Cohen
Jonathan C
JC
Herz
Joachim
J
Hobbs
Helen H
HH
D000818:Animals; D002784:Cholesterol / Q000097:blood / Q000378:metabolism; D005808:Genes, Recessive; D006938:Hyperlipoproteinemia Type II / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D008074:Lipoproteins / Q000097:blood / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000097:blood*; D008079:Lipoproteins, VLDL / Q000097:blood* / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D011973:Receptors, LDL / Q000097:blood* / Q000172:deficiency / Q000235:genetics
D008074:Lipoproteins; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D011973:Receptors, LDL; D002784:Cholesterol