title: Preliminary structural studies of the transcriptional regulator CmeR from Campylobacter jejuni.
abstract: In Campylobacter jejuni, a gram-negative bacterial pathogen causing gastroenteritis in humans, the CmeR regulatory protein controls transcription of the multidrug transporter gene operon cmeABC. CmeR belongs to the TetR family of transcriptional regulators. The 210-residue CmeR consists of two functional motifs: an N-terminal DNA-binding domain and a C-terminal ligand-binding domain. It is predicted that the DNA-binding domain interacts directly with target promoters, while the C-terminal motif interacts with inducing ligands (such as bile salts). As an initial step towards confirming this structural model, recombinant CmeR protein containing a 6 x His tag at the N-terminus was crystallized. Crystals of ligand-free CmeR belonged to space group P2(1)2(1)2, with unit-cell parameters a = 37.4, b = 57.6, c = 93.3 A. Diffraction was observed to at least 2.2 A at 100 K. Analysis of the detailed CmeR structure is currently in progress.
authors.affiliation: Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA.
authors.forename: Chih-Chia
authors.initials: CC
authors.lastname: Su
authors.forename: Feng
authors.initials: F
authors.lastname: Shi
authors.forename: Ruoyu
authors.initials: R
authors.lastname: Gu
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Gerry
authors.initials: G
authors.lastname: McDermott
authors.forename: Edward W
authors.initials: EW
authors.lastname: Yu
authors.forename: Qijing
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D016123:Campylobacter jejuni / Q000737:chemistry / Q000235:genetics*; D018360:Crystallography, X-Ray / Q000379:methods*; D050436:Regulatory Elements, Transcriptional / Q000235:genetics*; D014158:Transcription, Genetic / Q000502:physiology