title: A ventricular pressure-clamping system for the study of mechano-electrical feedback.
abstract: It is necessary to control the mechanical stimuli precisely in the studies of cardiac mechano-electrical feedback (MEF). In the present study a ventricular pressure-clamping system has been developed, which can be applied to isolated-perfused rabbit hearts. Controlled by a computer, this system not only can make the left ventricle follow a command defining the same pressure wave as that during a beating cycle under physiological condition, but also deliver mechanical stimuli with a proper waveform to the ventricle at a particular time phase. This system integrates multiple functions, including perfusing, pacing, recording of electrocardiogram and monophasic action potentials, and clamping and measuring of ventricular pressures in isolated-perfused hearts. Thus, it is a distinct system for investigating the phenomena and mechanisms of cardiac MEF at organ level.
authors.affiliation: Department of Physiology, Capital Medical University, Beijing 100069, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wei
authors.forename: Hai-Xia
authors.initials: HX
authors.lastname: Huang
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Zhi-Fei
authors.initials: ZF
authors.lastname: Zhang
authors.forename: Xiao-Suo
authors.initials: XS
authors.lastname: Fu
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Wei-Zhen
authors.initials: WZ
authors.lastname: Niu
mesh_terms: D000200:Action Potentials; D000818:Animals; D003250:Constriction; D004562:Electrocardiography; D005246:Feedback; D006321:Heart / Q000502:physiology*; D066298:In Vitro Techniques; D011817:Rabbits; D017725:Ventricular Pressure