Poly(ADP-ribose)-polymerase-1 and aging: experimental study of possible relationship on stationary cell cultures.
Experiments on "stationary aging" cultures of B11dii-FAF28 Chinese hamster cells showed that contact inhibition of cell growth and further culturing of cells in the stationary phase led to continuous inhibition of enzymes realizing poly-ADP-ribosylation of chromatin proteins. Cell density in the monolayer and percentage of damaged cells detected by trypan blue staining decreases during this process.
Shram
S I
SI
Institute of Molecular Genetics, Russian Academy of Sciences. shram@img.ras.ru
Shilovskii
G A
GA
Khokhlov
A N
AN
D000818:Animals; D016466:CHO Cells; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000502:physiology; D016922:Cellular Senescence / Q000502:physiology*; D002843:Chromatin / Q000378:metabolism*; D003260:Contact Inhibition / Q000502:physiology*; D006224:Cricetinae; D003412:Cricetulus; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*
D002843:Chromatin; D011065:Poly(ADP-ribose) Polymerases