[Identification of the degradation compounds of cefathiamidine by liquid chromatography-tandam mass spectrometry].
OBJECTIVE
To identify the degradation compounds of cefathiamidine by a liquid chromatography-tandam mass spectrometry (LC-MS).


METHODS
According to the accelerated storage condition, two main degradation compounds of cefathiamindine were its hydrolytic products. Cefathiamidine was separated on a C18 column, with 1% acetate solution-acetonitrile (85 : 15) as mobile phase. The mass spectra and MS/MS spectra were obtained with set at the positive ion mode. Combined the UV spectra and the chromatography behavior, the structures of two degradation compounds were identified.


RESULTS
The method can be used for the separation of the degradation compounds of cefathiamindine. The two major degradation compounds were desacetylcefathiamidine and cefathiamidine lactone.


CONCLUSIONS
The method is rapid, sensitive and specific, and it is suitable for the identification of the degradation compounds of cephalosporin.
Hu
Min
M
National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China.
Hu
Chang-Qin
CQ
D000900:Anti-Bacterial Agents / Q000032:analysis / Q000378:metabolism*; D002511:Cephalosporins / Q000032:analysis / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000900:Anti-Bacterial Agents; D002511:Cephalosporins; C018866:cefathiamidine