Quaternary structure and biochemical properties of mycobacterial RNase E/G.
The RNase E/G family of endoribonucleases plays the central role in numerous post-transcriptional mechanisms in Escherichia coli and, presumably, in other bacteria, including human pathogens. To learn more about specific properties of RNase E/G homologues from pathogenic Gram-positive bacteria, a polypeptide comprising the catalytic domain of Mycobacterium tuberculosis RNase E/G (MycRne) was purified and characterized in vitro. In the present study, we show that affinity-purified MycRne has a propensity to form dimers and tetramers in solution and possesses an endoribonucleolytic activity, which is dependent on the 5'-phosphorylation status of RNA. Our data also indicate that the cleavage specificities of the M. tuberculosis RNase E/G homologue and its E. coli counterpart are only moderately overlapping, and reveal a number of sequence determinants within MycRne cleavage sites that differentially affect the efficiency of cleavage. Finally, we demonstrate that, similar to E. coli RNase E, MycRne is able to cleave in an intercistronic region of the putative 9S precursor of 5S rRNA, thus suggesting a common function for RNase E/G homologues in rRNA processing.
Zeller
Mirijam-Elisabeth
ME
Max F. Perutz Laboratories, Department of Microbiology and Immunobiology, University Departments at the Vienna Biocenter, Dr. Bohrgasse 9/4, A-1030 Vienna, Austria.
Csanadi
Agnes
A
Miczak
Andras
A
Rose
Thierry
T
Bizebard
Thierry
T
Kaberdin
Vladimir R
VR
D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D002850:Chromatography, Gel; D003001:Cloning, Molecular; D004722:Endoribonucleases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000201:enzymology; D007700:Kinetics; D009163:Mycobacterium bovis / Q000201:enzymology*; D020836:Protein Structure, Quaternary; D012329:RNA, Bacterial / Q000235:genetics
D001426:Bacterial Proteins; D012329:RNA, Bacterial; D004722:Endoribonucleases; C022537:ribonuclease E