Sensitivity of expression of perivitelline membrane glycoprotein ZP1 mRNA in the liver of Japanese quail (Coturnix japonica) to estrogenic compounds.
Avian perivitelline membrane protein, ZP1, is synthesized and secreted by the liver with the stimulation of estrogens. In the present study, we measured the expression of ZP1 gene in the liver of immature male quail treated with various estrogenic compounds and in the liver of male quail embryos that were developed in the fertilized eggs laid by mother quail injected with various estrogenic compounds during vitellogenesis. Total RNA extracted from the liver was reverse-transcribed and cDNA was subjected to real-time PCR. Both diethylstilbestrol and ethinyl estradiol caused significant effect on the increase in mRNA in immature male quail. In contrast, diethylstilbestrol administered via the route of maternal injection was not effective for induction of embryonic mRNA, although the effect of ethinyl estradiol administered via the same route was prominent. These results showed that direct administration of estrogenic compounds, diethylstilbestrol and ethinyl estradiol, stimulates the induction of ZP1 gene, but the rate of accumulation of these compounds in the yolk is different during vitellogenesis. The present studies suggest that although ZP1 gene is a sensitive biomarker to evaluate the effects of endocrine disruptors, the route of administration is an important factor to compare the effectiveness.
Hanafy
Ahmed M
AM
The United Graduate School of Agricultural Science, Gifu University, Yanagido, Gifu 501-1193, Japan.
Sasanami
Tomohiro
T
Mori
Makoto
M
D000818:Animals; D001559:Benzhydryl Compounds; D003370:Coturnix / Q000196:embryology / Q000378:metabolism*; D003375:Coumestrol / Q000633:toxicity; D004054:Diethylstilbestrol / Q000633:toxicity; D004527:Egg Proteins / Q000235:genetics*; D004967:Estrogens / Q000633:toxicity*; D004997:Ethinyl Estradiol / Q000633:toxicity; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D019833:Genistein / Q000633:toxicity; D008099:Liver / Q000187:drug effects* / Q000196:embryology / Q000378:metabolism; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics*; D010636:Phenols / Q000633:toxicity; D012333:RNA, Messenger / Q000096:biosynthesis; D011956:Receptors, Cell Surface / Q000235:genetics*; D000072216:Zona Pellucida Glycoproteins; D015053:Zygote / Q000187:drug effects / Q000378:metabolism; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D003375:Coumestrol; D004054:Diethylstilbestrol; D004527:Egg Proteins; D004967:Estrogens; D004997:Ethinyl Estradiol; D019833:Genistein; D008562:Membrane Glycoproteins; D010636:Phenols; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D000072216:Zona Pellucida Glycoproteins; C025256:nonylphenol; C006780:bisphenol A; D000098826:Bisphenol A Compounds