Studies on the protective immunity of Schistosoma japonicum bivalent DNA vaccine encoding Sj23 and Sj14.
In order to explore the high performance bivalent DNA vaccine of Schistosoma japonicum, the fatty-acid-binding protein (Sj14) and the 23 kDa transmembrane protein (Sj23) two proteins were selected to construct the DNA-based vaccine. It was successful to construct a bivalent DNA vaccine using three strategies: the co-expression of two genes, a fusion gene expression and two kinds of plasmids in combination (cocktail vaccine). The bivalent DNA was proven to express well in vitro and in vivo by indirect immunofluorescence test (IIF) and reverse transcriptase-polymerase chain reaction (RT-PCR). The protective immunity of bivalent DNA vaccine was higher than that of univalent DNA vaccine (p<0.05). There were four groups of bivalent vaccine whose protective immunity was higher than 50%. Granuloma diameter reduction rates were in the range of 18-39%. There was no significant impact on immunity protection exerted by the four factors including dosage, inoculated times, inoculated routes and challenge time after the last immunization in three levels (p>0.05).
Yuan
Hu
H
Department of Parasitology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
You-En
Shi
S
Long-Jiang
Yu
Y
Xiao-Hua
Zhu
Z
Liu-Zhe
Li
L
Cash
Melanie
M
Lu
Zhu
Z
Zhi
Liu
L
Deng-Xin
Song
S
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000276:immunology*; D015870:Gene Expression; D015801:Helminth Proteins / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011897:Random Allocation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012549:Schistosoma japonicum / Q000235:genetics / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000517:prevention & control*; D012908:Snails; D019444:Vaccines, DNA / Q000276:immunology / Q000592:standards
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D050556:Fatty Acid-Binding Proteins; D015801:Helminth Proteins; D007074:Immunoglobulin G; D008565:Membrane Proteins; D019444:Vaccines, DNA; C072633:Sj23 antigen, Schistosoma japonicum