title: Negative cross-reactivity of rabbit anti-Malassezia furfur antibodies with other yeasts.
abstract: Anti-Malassezia furfur monospecific polyclonal antibodies was produced by repeated immunization of rabbit with Malassezia furfur yeast cells mixed with Freud adjuvant. The antibody titres of respective rabbit's serum samples prior to and after each immunization against M. furfur were assayed by indirect immunofluorescence technique using the M. furfur whole yeast antigen fixed in Teflon coated slides. The highest anti-M. furfur antibody titre achieved was 1 in 1280 dilution. At 1:20 dilution, none of the respective serum samples taken at various stages of immunization gave positive immunofluorescent staining against any of the other species of yeasts tested in this study. Anti-M. furfur monospecific polyclonal antibodies produced in rabbit in this study has the potential for diagnostic application in immunohistochemical detection of M. furfur in human tissues.
authors.affiliation: International Medical University, Kuala Lumpur. chuakawbing@yahoo.com.sg
authors.forename: Kaw Bing
authors.initials: KB
authors.lastname: Chua
authors.forename: Shamala
authors.initials: S
authors.lastname: Devi
authors.forename: Kee Peng
authors.initials: KP
authors.lastname: Ng
authors.forename: Poh Sim
authors.initials: PS
authors.lastname: Hooi
authors.forename: Shiang Ling
authors.initials: SL
authors.lastname: Na
authors.forename: Kerk Hsiang
authors.initials: KH
authors.lastname: Chua
mesh_terms: D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology*; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D006801:Humans; D008290:Malassezia / Q000276:immunology*; D011817:Rabbits; D014010:Tinea Versicolor / Q000175:diagnosis; D015003:Yeasts / Q000276:immunology*
chemical_list: D000908:Antibodies, Fungal