Localization and expression of selenoprotein S in the testis of Psammomys obesus.
Selenium is an essential trace element and selenoprotein S is a member of the selenoprotein family that has the non-standard amino acid selenocysteine incorporated into the polypeptide. Dietary selenium has been shown to play an important protective role in a number of diseases including cancer, immune function and the male reproductive system. In this study, we have observed high levels of selenoprotein S gene expression in the testis from Psammomys obesus. Real-time PCR and immunofluorescence demonstrate that selenoprotein S expression is low in testes from 4-week-old animals but increases significantly by 8 weeks of age and remains high until 17 weeks of age. Selenoprotein S protein is detected in primary spermatocytes, Leydig and Sertoli cells of 8, 12 and 17-week-old animals. These results suggest that selenoprotein S may play a role in spermatogenesis.
Windmill
Kelly
K
Metabolic Research Unit, School of Exercise and Nutrition Sciences, Deakin University, Pigdons Road, Waurn Ponds, VIC 3217, Australia. kellyw@deakin.edu.au
Tenne-Brown
Janette
J
Bayles
Richard
R
Trevaskis
James
J
Gao
Yuan
Y
Walder
Ken
K
Collier
Greg R
GR
D000375:Aging / Q000502:physiology*; D000818:Animals; D019587:Dietary Supplements; D005849:Gerbillinae / Q000378:metabolism*; D008297:Male; D012643:Selenium / Q000494:pharmacology; D017279:Selenocysteine / Q000378:metabolism; D051140:Selenoproteins / Q000378:metabolism*; D013091:Spermatogenesis / Q000187:drug effects / Q000502:physiology*; D013737:Testis / Q000166:cytology / Q000473:pathology*
D051140:Selenoproteins; D017279:Selenocysteine; D012643:Selenium