title: Statistical optimization of medium components for avilamycin production by Streptomyces viridochromogenes Tü57-1 using response surface methodology.
abstract: A fermentation medium for avilamycin production by Streptomyces viridochromogenes Tü57-1 has been optimized. Important components and their concentrations were investigated using fractional factorial design and Box-Behnken Design. The results showed that soybean flour, soluble starch, MgSO4.7H2O and CaCl2.2H2O are important for avilamycin production. A polynomial model related to medium components and avilamycin yield had been established. A high coefficient of determination (R2 = 0.92) was obtained that indicated good agreement between the experimental and predicted values of avilamycin yield. Student's T-test of each coefficient showed that all the linear and quadratic terms had significant effect (P > |T| < 0.05) on avilamycin yield. The significance of tested components was related to MgSO4.7H2O (0.37 g/L), CaCl2.2H2O (0.39 g/L), soybean flour (21.97 g/L) and soluble starch (37.22 g/L). The yield of avilamycin reached 88.33 +/- 0.94 mg/L (p < 0.05) that was 2.8-fold the initial yield.
authors.affiliation: College of Food Science and Engineering, Shangdong Agriculture University, Taian 271018, China.
authors.forename: Chuan-He
authors.initials: CH
authors.lastname: Zhu
authors.forename: Fu-Ping
authors.initials: FP
authors.lastname: Lu
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: He
authors.forename: Juan-Kun
authors.initials: JK
authors.lastname: Zhang
authors.forename: Lian-Xiang
authors.initials: LX
authors.lastname: Du
mesh_terms: D018533:Biomass; D002244:Carbon / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism* / Q000494:pharmacology; D005285:Fermentation / Q000187:drug effects; D007218:Industrial Microbiology / Q000379:methods; D009584:Nitrogen / Q000378:metabolism; D009844:Oligosaccharides / Q000096:biosynthesis*; D015203:Reproducibility of Results; D013302:Streptomyces / Q000254:growth & development / Q000378:metabolism*
chemical_list: D003470:Culture Media; D009844:Oligosaccharides; C006653:avilamycin; D002244:Carbon; D009584:Nitrogen