Differences in microbiological composition of saliva and dental plaque in subjects with different drinking habits.
Several foods have been shown to contain natural components (especially polyphenols) which display anti-adhesive properties against Streptococcus mutans, the aetiological agent responsible for dental crown caries, as well as inhibition of glucosyltransferases, which are the S. mutans enzymes involved in the synthesis of an adherent, water-insoluble glucan from sucrose. Other studies have demonstrated an in vitro action on oral plaque biofilm formation and desorption. This study evaluated whether the activity displayed in vitro by food compounds could affect the microbiological composition of saliva and dental plaque of subjects with a diet rich in these foods, comparing the results with those obtained from subjects with a different diet. The foods considered were: coffee, barley coffee, tea and wine. A total of 93 subjects were recruited into the study. Six samples of both plaque and saliva were collected from each subject at roughly one-monthly intervals. Total bacteria, total streptococci, S. mutans and lactobacilli counts were determined by culture in both saliva and dental plaque. The highest bacterial titres were recorded for the control population, while each drinking habit subgroup showed counts roughly one log lower than the controls. These differences in bacterial counts proved statistically significant (P<0.05). As far as dental plaque was concerned, while total counts did not significantly vary per mg of plaque in the subjects belonging to the different drinking habit subgroups, a significant decrease (P<0.05) was observed in those subjects drinking coffee, tea, barley coffee and wine when mutans streptococci and lactobacilli were evaluated. In several cases a more than one log decrease was observed. Plaque indices were also determined, and a significant (P<0.05) reduction in values was recorded in the subjects belonging the specific drinking habit subgroups compared to the control group. This study indicates that there is a correlation between consumption of specific foods and oral health in terms of reduced plaque deposition and lower counts of odontopathogens.
Signoretto
Caterina
C
Dipartimento di Patologia, Sezione di Microbiologia, Strada Le Grazie, Italy.
Burlacchini
Gloria
G
Bianchi
Franco
F
Cavalleri
Giacomo
G
Canepari
Pietro
P
D000328:Adult; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification*; D003069:Coffee; D015169:Colony Count, Microbial; D004245:DMF Index; D003773:Dental Plaque / Q000382:microbiology*; D005247:Feeding Behavior; D005260:Female; D001467:Hordeum; D006801:Humans; D007778:Lactobacillus / Q000302:isolation & purification; D008297:Male; D008875:Middle Aged; D009910:Oral Hygiene; D012463:Saliva / Q000382:microbiology*; D013291:Streptococcus / Q000302:isolation & purification; D013295:Streptococcus mutans / Q000302:isolation & purification; D013662:Tea; D014920:Wine
D003069:Coffee; D013662:Tea