The pathogenicity of mosquito densovirus (C6/36DNV) and its interaction with dengue virus type II in Aedes albopictus.
When Aedes albopictus larvae were infected with C6/36 densovirus (C6/36DNV), the mortality reached 97.46% within 21 days for those larvae infected at the first day after hatching, and 14.17% for control. A real-time quantitative polymerase chain reaction (qPCR) was used to trace the dynamic change of the quantity of C6/36DNV genomes in the larvae and the adults, and to study the interaction of C6/36DNV with dengue virus type II(DEN-II) in mosquitoes. It showed that C6/36DNV could persist in the adults that could transmit C6/36DNV vertically to the next generation. The quantity of C6/36DNV after DEN-II infection increased by 10(2)~10(3) times in the C6/36DNV-positive mosquitoes, and the quantity of DEN-II in the C6/36DNV-positive mosquitoes was about 100 times lower than that in the C6/36DNV-negative mosquitoes, which suggested that DEN-II could remarkably stimulate the reproduction of C6/36DNV, while C6/36DNV persisted in mosquitoes could inhibit the reproduction of DEN-II. The study throws light on C6/36DNV as a possible biologic control agent against dengue virus and Ae. albopictus.
Wei
Wei
W
State Key Lab for Biocontrol and Institute of Entomology, Sun Yat-Sen University, Guangzhou, PR China.
Shao
Dingyong
D
Huang
Xiaojun
X
Li
Jianping
J
Chen
Haidong
H
Zhang
Qinfen
Q
Zhang
Jingqiang
J
D000330:Aedes / Q000254:growth & development / Q000502:physiology / Q000821:virology*; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D003716:Dengue Virus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D017990:Densovirus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D006801:Humans; D007814:Larva / Q000821:virology; D010322:Parvoviridae Infections / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D012098:Reproduction
D017931:DNA Primers; D012367:RNA, Viral