Effects of low-dose irradiation on enhancement of immunity by dendritic cells.
Low-doses of irradiation have been reported to have beneficial effects, particularly anti-tumor effects. In this paper, we show the effects of the low-dose irradiation on T cell activation induced by dendritic cells (DCs). DCs, which had been pre-irradiated at 0.02-1.0 Gy from a (137)Cs source, were cultured with allogeneic T cells, and the proliferation of T cells was then examined. The 0.05Gy-pre-irradiated DCs showed the highest proliferation capacity of T cells. The 0.05Gy-irradiation does not augment the expression of major histocompatibility complexes (MHCs) or costimulatory molecules on DCs, as with non-irradiated DCs or 1Gy-irradiated DCs, but does augment the production of IL-2, IL-12 and IFN-gamma DCs. These results suggest that the low-dose irradiation augments T cell-activation capacity through cytokine production by DCs, which might shift naïve helper T cells to Th1 cells.
Shigematsu
Akio
A
First Department of Pathology, Kansai Medical University, Osaka, Japan.
Adachi
Yasushi
Y
Koike-Kiriyama
Naoko
N
Suzuki
Yasuhiro
Y
Iwasaki
Masayoshi
M
Koike
Yasushi
Y
Nakano
Keiji
K
Mukaide
Hiromi
H
Imamura
Masahiro
M
Ikehara
Susumu
S
D000818:Animals; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology* / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D015870:Gene Expression / Q000276:immunology / Q000528:radiation effects; D007113:Immunity, Innate / Q000528:radiation effects*; D008213:Lymphocyte Activation / Q000276:immunology / Q000528:radiation effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011829:Radiation Dosage; D013601:T-Lymphocytes / Q000276:immunology* / Q000528:radiation effects*
D016207:Cytokines