Marker-exchange mutagenesis and complementation strategies for the Gram-negative bacteria Xanthomonas oryzae pv. oryzae.
This chapter describes methods for targeted knockouts using marker exchange mutagenesis and complementation of the Gram-negative bacteria Xanthomonas oryzae pv. oryzae. We have used these methods to demonstrate that type I secretion and modification systems are involved in avrXa21 activity of X. oryzae pv. oryzae.
Lee
Sang-Won
SW
Department of Plant Pathology, University of California, Davis, USA.
Ronald
Pamela C
PC
D015870:Gene Expression; D005816:Genetic Complementation Test / Q000379:methods*; D005819:Genetic Markers / Q000235:genetics; D016296:Mutagenesis / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D014974:Xanthomonas / Q000235:genetics*
D005819:Genetic Markers