title: Dynamic changes of gangliosides expression during the differentiation of embryonic and mesenchymal stem cells into neural cells.
abstract: Stem cells are used for the investigation of developmental processes at both cellular and organism levels and offer tremendous potentials for clinical applications as an unlimited source for transplantation. Gangliosides, sialic acid-conjugated glycosphingolipids, play important regulatory roles in cell proliferation and differentiation. However, their expression patterns in stem cells and during neuronal differentiation are not known. Here, we investigated expression of gangliosides during the growth of mouse embryonic stem cells (mESCs), mesenchymal stem cells (MSCs) and differentiated neuronal cells by using high-performance thin-layer chromatography (HPTLC). Monosialoganglioside 1 (GM1) was expressed in mESCs and MSCs, while GM3 and GD3 were expressed in embryonic bodies. In the 9-day old differentiated neuronal cells from mESCs cells and MSCs, GM1 and GT1b were expressed. Results from immunostaining were consistent with those observed by HPTLC assay. These suggest that gangliosides are specifically expressed according to differentiation of mESCs and MSCs into neuronal cells and expressional difference of gangliosides may be a useful marker to identify differentiation of mESCs and MSCs into neuronal cells.
authors.affiliation: Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan 570-749, Korea.
authors.forename: Dong-Hoon
authors.initials: DH
authors.lastname: Kwak
authors.forename: Kweon
authors.initials: K
authors.lastname: Yu
authors.forename: Sung-Min
authors.initials: SM
authors.lastname: Kim
authors.forename: Dea-Hoon
authors.initials: DH
authors.lastname: Lee
authors.forename: Sun-Mi
authors.initials: SM
authors.lastname: Kim
authors.forename: Ji-Ung
authors.initials: JU
authors.lastname: Jung
authors.forename: Jung-Woo
authors.initials: JW
authors.lastname: Seo
authors.forename: Nari
authors.initials: N
authors.lastname: Kim
authors.forename: Seoul
authors.initials: S
authors.lastname: Lee
authors.forename: Kyu-Yong
authors.initials: KY
authors.lastname: Jung
authors.forename: Hyung-Keun
authors.initials: HK
authors.lastname: You
authors.forename: Hyun-A
authors.initials: HA
authors.lastname: Kim
authors.forename: Young-Kug
authors.initials: YK
authors.lastname: Choo
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D005732:Gangliosides / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*
chemical_list: D005732:Gangliosides