title: Decompression sickness in the rat following a dive on trimix: recompression therapy with oxygen vs. heliox and oxygen.
abstract: Trimix (a mixture of helium, nitrogen, and oxygen) has been used in deep diving to reduce the risk of high-pressure nervous syndrome during compression and the time required for decompression at the end of the dive. There is no specific recompression treatment for decompression sickness (DCS) resulting from trimix diving. Our purpose was to validate a rat model of DCS on decompression from a trimix dive and to compare recompression treatment with oxygen and heliox (helium-oxygen). Rats were exposed to trimix in a hyperbaric chamber and tested for DCS while walking in a rotating wheel. We first established the experimental model, and then studied the effect of hyperbaric treatment on DCS: either hyperbaric oxygen (HBO) (1 h, 280 kPa oxygen) or heliox-HBO (0.5 h, 405 kPa heliox 50%-50% followed by 0.5 h, 280 kPa oxygen). Exposure to trimix was conducted at 1,110 kPa for 30 min, with a decompression rate of 100 kPa/min. Death and most DCS symptoms occurred during the 30-min period of walking. In contrast to humans, no permanent disability was found in the rats. Rats with a body mass of 100-150 g suffered no DCS. The risk of DCS in rats weighing 200-350 g increased linearly with body mass. Twenty-four hours after decompression, death rate was 40% in the control animals and zero in those treated immediately with HBO. When treatment was delayed by 5 min, death rate was 25 and 20% with HBO and heliox, respectively.
authors.affiliation: Israel Naval Medical Institute, POB 8040, Haifa 31080, Israel. rarieli@netvision.net.il
authors.forename: R
authors.initials: R
authors.lastname: Arieli
authors.forename: P
authors.initials: P
authors.lastname: Svidovsky
authors.forename: A
authors.initials: A
authors.lastname: Abramovich
mesh_terms: D000818:Animals; D003198:Computer Simulation; D003665:Decompression Sickness / Q000209:etiology / Q000503:physiopathology* / Q000628:therapy*; D004195:Disease Models, Animal; D004242:Diving / Q000009:adverse effects*; D004338:Drug Combinations; D006371:Helium / Q000008:administration & dosage*; D006931:Hyperbaric Oxygenation / Q000379:methods*; D008297:Male; D008954:Models, Biological; D009584:Nitrogen / Q000008:administration & dosage*; D010100:Oxygen / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016896:Treatment Outcome
chemical_list: D004338:Drug Combinations; D006371:Helium; C041364:nitrox; C038949:heliox; C045257:trimix; D009584:Nitrogen; D010100:Oxygen