title: Transcriptional profiling of human cord blood CD133+ and cultured bone marrow mesenchymal stem cells in response to hypoxia.
abstract: Umbilical cord blood (UCB) and bone marrow (BM)-derived stem and progenitor cells possess two characteristics required for successful tissue regeneration: extensive proliferative capacity and the ability to differentiate into multiple cell lineages. Within the normal BM and in pathological conditions, areas of hypoxia may have a role in maintaining stem cell fate or determining the fine equilibrium between their proliferation and differentiation. In this study, the transcriptional profiles and proliferation and differentiation potential of UCB CD133(+) cells and BM mesenchymal cells (BMMC) exposed to normoxia and hypoxia were analyzed and compared. Both progenitor cell populations responded to hypoxic stimuli by stabilizing the hypoxia inducible factor (HIF)-1alpha protein. Short exposures to hypoxia increased the clonogenic myeloid capacity of UCB CD133(+) cells and promoted a significant increase in BMMC number. The differentiation potential of UCB CD133(+) clonogenic myeloid cells was unaltered by short exposures to hypoxia. In contrast, the chondrogenic differentiation potential of BMMCs was enhanced by hypoxia, whereas adipogenesis and osteogenesis were unaltered. When their transcriptional profiles were compared, 183 genes in UCB CD133(+) cells and 45 genes in BMMC were differentially regulated by hypoxia. These genes included known hypoxia-responsive targets such as BNIP3, PGK1, ENO2, and VEGFA, and other genes not previously described to be regulated by hypoxia. Several of these genes, namely CDTSPL, CCL20, LSP1, NEDD9, TMEM45A, EDG-1, and EPHA3 were confirmed to be regulated by hypoxia using quantitative reverse transcriptase polymerase chain reaction. These results, therefore, provide a global view of the signaling and regulatory network that controls oxygen sensing in human adult stem/progenitor cells derived from hematopoietic tissues.
authors.affiliation: Stem Cell Research Laboratory, National Blood Service, Oxford Centre, The John Radcliffe Hospital, Headington, OX3 9BQ, United Kingdom. enca.martin-rendon@nbs.nhs.uk
authors.forename: Enca
authors.initials: E
authors.lastname: Martin-Rendon
authors.forename: Sarah J M
authors.initials: SJ
authors.lastname: Hale
authors.forename: Dacey
authors.initials: D
authors.lastname: Ryan
authors.forename: Dilair
authors.initials: D
authors.lastname: Baban
authors.forename: Sinead P
authors.initials: SP
authors.lastname: Forde
authors.forename: Maria
authors.initials: M
authors.lastname: Roubelakis
authors.forename: Dominic
authors.initials: D
authors.lastname: Sweeney
authors.forename: Meis
authors.initials: M
authors.lastname: Moukayed
authors.forename: Adrian L
authors.initials: AL
authors.lastname: Harris
authors.forename: Kay
authors.initials: K
authors.lastname: Davies
authors.forename: Suzanne M
authors.initials: SM
authors.lastname: Watt
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD / Q000032:analysis; D001854:Bone Marrow Cells / Q000166:cytology*; D002455:Cell Division; D015687:Cell Hypoxia; D003114:Colony-Forming Units Assay; D005312:Fetal Blood / Q000166:cytology* / Q000502:physiology*; D020869:Gene Expression Profiling; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D007231:Infant, Newborn; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology*; D010455:Peptides / Q000032:analysis; D014158:Transcription, Genetic
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides