Tetraphenylphosphonium-selective electrode as a tool for evaluating mitochondrial permeability transition pore function in isolated rat hepatocytes.
The changes in mitochondrial membrane potential (Deltapsi(m)) were used as an indicator for evaluating the mitochondrial permeability transition pore (MPTP) function. We found that in situ mitochondria in digitonin-permeabilized hepatocytes were coupled and responded to the addition of substrates, inhibitors and uncouplers. Ca(2+)-induced Deltapsi(m) dissipation was caused by MPTP opening because this process was inhibited by cyclosporin A. MPTP opening was enhanced by the pro-oxidant tert-butyl hydroperoxide.
Lábajová
A
A
Institute of Pathophysiology, 1st Faculty of Medicine, Charles University, U Nemocnice 5, 120 00 Prague 2, Czech Republic. Anna.Labajova@lf1.cuni.cz
Kofránek
J
J
Kriváková
P
P
Cervinková
Z
Z
Drahota
Z
Z
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D004566:Electrodes; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D053078:Membrane Potential, Mitochondrial; D033681:Mitochondrial Membrane Transport Proteins / Q000378:metabolism*; D000083162:Mitochondrial Permeability Transition Pore; D009861:Onium Compounds; D009943:Organophosphorus Compounds; D010101:Oxygen Consumption; D051381:Rats; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
D007202:Indicators and Reagents; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D009861:Onium Compounds; D009943:Organophosphorus Compounds; D020122:tert-Butylhydroperoxide; D002118:Calcium; C013289:tetraphenylphosphonium