title: Non-NMDA receptors in frog retina: an immunocytochemical study.
abstract: Glutamate is one of the main neurotransmitters in the retina. Its effects are mediated by a large number of ionotropic and metabotropic membrane receptors. The distribution of ionotropic AMPA receptor subunits GluR1-4, kainate receptor subunits GluR5-7 and KA2, delta receptors 1-2, as well as the metabotropic receptor mGluR6 were studied in the frog retina. Indirect immunofluorescence was used to localize the different receptor subunits. Results showed that all subunits, with the exception of GluR1 and GluR5, are widely distributed in the retina. They are mainly located in both plexiform layers: the outer (OPL) and the inner one (IPL), where punctate labelling, a sign of synaptic localization, is observed. The metabotropic receptor mGluR6 is localised only in the OPL. The AMPA receptor subunit GluR4 is localised on the glial Müller cells of the retina. The vast majority of the subunits possess specific patterns of labelling that indicate that they are involved with different retinal functions. The significance of the AMPA receptors and involvement of glia in modulation of synaptic transmission are discussed.
authors.affiliation: Department of Physiology, Medical Faculty, Medical University, Sofia, Bulgaria. lilyvita@abv.bg
authors.forename: Lily
authors.initials: L
authors.lastname: Vitanova
mesh_terms: D000818:Animals; D007150:Immunohistochemistry / Q000379:methods; D008856:Microscopy, Fluorescence; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D011895:Rana ridibunda; D018091:Receptors, AMPA / Q000032:analysis; D018092:Kainic Acid Receptors / Q000032:analysis; D018094:Receptors, Metabotropic Glutamate / Q000032:analysis; D016194:Receptors, N-Methyl-D-Aspartate / Q000032:analysis*; D017465:Receptors, Opioid, delta / Q000032:analysis; D012160:Retina / Q000378:metabolism*; D000097806:GluK2 Kainate Receptor; D000097808:GluK3 Kainate Receptor
chemical_list: D018091:Receptors, AMPA; D018092:Kainic Acid Receptors; D018094:Receptors, Metabotropic Glutamate; D016194:Receptors, N-Methyl-D-Aspartate; D017465:Receptors, Opioid, delta; D000097806:GluK2 Kainate Receptor; D000097808:GluK3 Kainate Receptor; C104723:glutamate receptor ionotropic, AMPA 3; C104724:glutamate receptor ionotropic, AMPA 4; C113897:metabotropic glutamate receptor 6; C104722:glutamate receptor ionotropic, AMPA 2