Convenient method for monitoring Abeta aggregation by quartz-crystal microbalance.
We have found that a quartz-crystal microbalance is a facile and useful tool for detecting the specific aggregation of the amyloid-beta peptides responsible for Alzheimer's disease. This method greatly reduces the time and samples (corresponds to 1 Hz frequency decrease per 30 pg mass increase on a 4.9 mm(2) electrode) that are needed in the aggregation assay, and is also applicable to molecular interactions between smaller amyloid-beta fragment peptides such as the pentapeptide KLVFF.
Okuno
Hiroaki
H
Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1 Funabashi, Chiba 274-8510, Japan. okuno@phar.toho-u.ac.jp
Mori
Kanae
K
Jitsukawa
Tomofumi
T
Inoue
Hiromi
H
Chiba
Shinya
S
D000327:Adsorption; D000544:Alzheimer Disease; D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000032:analysis* / Q000378:metabolism; D019281:Dimerization; D004566:Electrodes; D006801:Humans; D008722:Methods; D008970:Molecular Weight; D011791:Quartz
D016229:Amyloid beta-Peptides; D011791:Quartz