title: Immunostimulatory principles from Chlorella pyrenoidosa--part 1: isolation and biological assessment in vitro.
abstract: Our proprietary preparation obtained by extraction of Chlorella pyrenoidosa cells, ONC-107 (Respondin), was recently found to selectively boost antibody response to the influenza vaccine in a human clinical trial. Respondin is a potent stimulator of mouse B cell proliferation and an activator of macrophages. Bioactivity-guided resolution concluded that Respondin is composed of a mixture of immunostimulatory principles of different chemical nature. A combination of size exclusion, anion exchange and hydrophobic interaction chromatography revealed that the bulk of the immunostimulatory activity resides in polysaccharide/protein complexes with molecular masses larger than 100 kDa that are composed primarily of galactose, rhamnose and arabinose.
authors.affiliation: Ocean Nutrition Canada Ltd., 101 Research Drive, Dartmouth, NS, B2Y 4T6. jkralovec@ocean-nutrition.com
authors.forename: J A
authors.initials: JA
authors.lastname: Kralovec
authors.forename: K L
authors.initials: KL
authors.lastname: Metera
authors.forename: J R
authors.initials: JR
authors.lastname: Kumar
authors.forename: L V
authors.initials: LV
authors.lastname: Watson
authors.forename: G S
authors.initials: GS
authors.lastname: Girouard
authors.forename: Y
authors.initials: Y
authors.lastname: Guan
authors.forename: R I
authors.initials: RI
authors.lastname: Carr
authors.forename: C J
authors.initials: CJ
authors.lastname: Barrow
authors.forename: H S
authors.initials: HS
authors.lastname: Ewart
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002708:Chlorella; D016207:Cytokines / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D007155:Immunologic Factors / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D066298:In Vitro Techniques; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D013154:Spleen / Q000166:cytology / Q000187:drug effects
chemical_list: D016207:Cytokines; D007155:Immunologic Factors; C518452:ONC-107; D010936:Plant Extracts