Subcellular fractionation.
The successful combination of highly sensitive mass spectrometry and pre-fractionation techniques has provided a powerful tool to detect dynamic changes in low abundant regulatory proteins at the organelle level. Subcellular fractionation, being flexible, adjustable (both in cell and tissues), and allowing the analysis of proteins in their physiologic/intracellular context, has become the most commonly used preparative/enrichment method. This chapter introduces state-of-the-art subcellular fractionation protocols and briefly discuss their suitability, advantages, and limitations.
de Araújo
Mariana Eça Guimarães
ME
Insbruck Biocentre, Austria.
Huber
Lukas Alfons
LA
D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D002498:Centrifugation; D006801:Humans; D015388:Organelles / Q000737:chemistry*; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification; D015203:Reproducibility of Results; D013347:Subcellular Fractions / Q000737:chemistry / Q000378:metabolism
D011506:Proteins