Adverse effects of citrate/gold nanoparticles on human dermal fibroblasts.
Nanoscale engineering is one of the most dynamically growing areas at the interface between electronics, physics, biology, and medicine. As there are no safety regulations yet, concerns about future health problems are rising. We investigated the effects of citrate/gold nanoparticles at different concentrations and exposure times on human dermal fibroblasts. We found that, as a result of intracellular nanoparticle presence, actin stress fibers disappeared, thereby inducing major adverse effects on cell viability. Thus, properties such as cell spreading and adhesion, cell growth, and protein synthesis to form the extracellular matrix were altered dramatically. These results suggest that the internal cell activities have been damaged.
Pernodet
Nadine
N
Materials Science and Engineering, SUNY at Stony Brook, Stony Brook, NY 11794-2275, USA. npernodet@notes.cc.sunysb.edu
Fang
Xiaohua
X
Sun
Yuan
Y
Bakhtina
Asya
A
Ramakrishnan
Aditi
A
Sokolov
Jonathan
J
Ulman
Abraham
A
Rafailovich
Miriam
M
D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D019343:Citric Acid / Q000009:adverse effects* / Q000737:chemistry; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects* / Q000502:physiology; D006046:Gold / Q000009:adverse effects* / Q000737:chemistry; D006801:Humans; D053758:Nanoparticles / Q000009:adverse effects* / Q000737:chemistry; D012867:Skin / Q000166:cytology* / Q000187:drug effects*
D019343:Citric Acid; D006046:Gold