Highly sensitive detection of HPV-DNA in paraffin sections of human oral carcinomas.
BACKGROUND
Although human papillomavirus (HPV) infection has been shown to be a significant carcinogen in cervical squamous cell carcinoma (SCC), its significance in oral SCC remains unclear.


METHODS
We developed highly sensitive detection methods for HPV to elucidate the prevalence and localization of HPV in paraffin sections from human oral SCC using modified in situ polymerase chain reaction (PCR) and in situ hybridization AT tailing (ISH-AT). Analyses revealed a high prevalence of several HPV types (HPV-16, -18, -22, -38 and -70) under optimal conditions. The ISH-AT method can be used as an alternative to in situ PCR.


RESULTS
Various staining patterns were observed in the 20 cases examined, and HPV-positive cells were localized within the surface epithelium as well as in neoplastic cells. We demonstrated that HPV-DNA could be detected in paraffin sections using either the method of in situ PCR or ISH, providing an appropriate primer and probe are used.


CONCLUSIONS
These results suggest that HPV infection could be one of several risk factors being involved in oral SCC.
Koyama
Koichi
K
Department of Oral Pathology, Osaka Dental University, Hirakata, Osaka, Japan.
Uobe
Kenichi
K
Tanaka
Akio
A
D020856:AT Rich Sequence / Q000235:genetics; D000328:Adult; D000368:Aged; D052685:Alphapapillomavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002294:Carcinoma, Squamous Cell / Q000821:virology*; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D005260:Female; D005882:Gingival Diseases / Q000821:virology; D052162:Human papillomavirus 16 / Q000302:isolation & purification; D052161:Human papillomavirus 18 / Q000302:isolation & purification; D006801:Humans; D017403:In Situ Hybridization; D007972:Leukoplakia, Oral / Q000821:virology; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000821:virology*; D030361:Papillomavirus Infections / Q000821:virology*; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction; D020401:Primed In Situ Labeling
D015344:DNA Probes, HPV; D004279:DNA, Viral