Effects of cholesterol ester transfer protein (CETP) gene on adiposity in response to long-term overfeeding.
Cholesterol ester transfer protein (CETP) plays a key role in remodeling triglyceride-rich particles and high-density lipoproteins (HDL). We investigated CETP sequence variants in response to long-term overfeeding (100 days) in 12 pairs of male monozygotic twins (mean age+/-S.D.: 21+/-2 years). Body fat mass (FM), abdominal subcutaneous (ASF) and visceral fat (AVF), and plasma lipoproteins were determined. The CETP variants C>T/In9 (rs289714) and G>A/Ex14 (rs5882, or I405V) were investigated by RFLP-PCR methodologies. Before overfeeding, the CETP CC/In9 (n=18) genotype was associated with lower FM compared to the C>T/In9 heterozygotes. Overfeeding induced more FM and ASF accretion in C>T/In9 carriers (P<or=0.05). CETP V405V homozygotes (n=8) had lower BMI, FM, and ASF before overfeeding than those with the I405I (n=6) or I405V (n=10) genotypes. However, V405V subjects had the largest gain in AVF with overfeeding (P=0.02). Decreases from baseline were significantly different across the I405V genotypes for HDL-C, HDL-Apo AI, HDL(2), and HDL(3) (P<or=0.05). Our data suggests that CETP sequence variation contributes to the undesirable changes in adiposity and HDL-C levels when exposed to excessive calorie consumption and may be potentially helpful to identify individuals with the metabolic syndrome who are at higher risk of cardiovascular disease.
Terán-García
Margarita
M
Pennington Biomedical Research Center, Louisiana State University System, 6400 Perkins Road, Baton Rouge, LA 70808, United States.
Després
Jean-Pierre
JP
Quebec Heart Institute, Laval Hospital Research Center, Ste-Foy (Québec), G1V 4G5, Canada; Physical Activity Sciences Laboratory, Division of Kinesiology, PEPS, Laval University, Ste-Foy (Québec), G1K 7P4, Canada.
Tremblay
Angelo
A
Physical Activity Sciences Laboratory, Division of Kinesiology, PEPS, Laval University, Ste-Foy (Québec), G1K 7P4, Canada.
Bouchard
Claude
C
Pennington Biomedical Research Center, Louisiana State University System, 6400 Perkins Road, Baton Rouge, LA 70808, United States. Electronic address: BouchaC@pbrc.edu.
D000273:Adipose Tissue; D000328:Adult; D053480:Cholesterol Ester Transfer Proteins / Q000235:genetics*; D015331:Cohort Studies; D004200:Diseases in Twins / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D006801:Humans; D006963:Hyperphagia / Q000235:genetics / Q000503:physiopathology; D008297:Male; D009765:Obesity / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D014430:Twins, Monozygotic; D015430:Weight Gain / Q000235:genetics*
C506642:CETP protein, human; D053480:Cholesterol Ester Transfer Proteins