High-yield activation of scaffold polymer surfaces to attach cell adhesion molecules.
Zirconium tetra(tert-butoxide) reacts with surface amide groups of polyamide nylon 6/6 to give (eta(2)-amidate)zirconium complexes in high yield. These surface complexes react to bond the cell-adhesive peptide arginine-glycine-aspartic acid (RGD) to the polymer surface. A surface loading of 0.18 nmol/cm(2) of RGD is achieved, which is 20-1000 times higher than previously reported attainable on natural or synthetic polymers by other strategies. Approximately 40% of the nylon surface is covered by the RGD, which gives a surface that is both stable to hydrolysis and highly active for cell adhesion and spreading in vitro.
Dennes
T Joseph
TJ
Department of Chemistry, Princeton University, Princeton, New Jersey 08544, USA.
Hunt
Geoffrey C
GC
Schwarzbauer
Jean E
JE
Schwartz
Jeffrey
J
D000818:Animals; D002209:Caprolactam / Q000031:analogs & derivatives* / Q000737:chemistry; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000737:chemistry*; D051379:Mice; D041681:NIH 3T3 Cells; D009842:Oligopeptides / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties
D015815:Cell Adhesion Molecules; D009842:Oligopeptides; D009942:Organometallic Compounds; D011108:Polymers; C518813:zirconium tetra(tert-butoxide); C009916:nylon 6; D002209:Caprolactam; C047981:arginyl-glycyl-aspartic acid