title: Phenotype comparison of three acetylcholinesterase knockout strains.
abstract: The phenotypes of three mouse strains that carry the acetylcholinesterase knockout (AChE KO) mutation have been compared. The AChE KO mouse was developed from embryonic stem (ES) cells, originating from a 129/Sv blastocyst. Animals generated from strain 129/Sv suffer from dysgenesis of the corpus callosum and possibly a number of other neuroanatomical deficiencies. To determine the contribution of background genes to phenotype, 129/Sv AChE heterozygote (AChE+/-) mice were backcrossed 10 generations with wild-type inbred C57/BL6 (C57) mice and with wild-type outbred CD1(R) mice. AChE-/- mice in strains C57 and CD1died during seizures before postnatal day (P) 21, whereas mice in strain 129/Sv lived to adulthood.
authors.affiliation: University of Nebraska Medical Center, Omaha, NE 68198-6805, USA. eduysen@unmc.edu
authors.forename: Ellen G
authors.initials: EG
authors.lastname: Duysen
authors.forename: Oksana
authors.initials: O
authors.lastname: Lockridge
mesh_terms: D000110:Acetylcholinesterase / Q000172:deficiency* / Q000235:genetics*; D000818:Animals; D001831:Body Temperature; D001835:Body Weight; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010641:Phenotype
chemical_list: D000110:Acetylcholinesterase