title: Genetic manipulation of CD74 in mouse strains of different backgrounds can result in opposite responses to central nervous system injury.
abstract: The ability to recover from CNS injuries is strain dependent. Transgenic mice that weakly express the p41 CD74 isoform (an integral membrane protein functioning as a MHC class II chaperone) on an I-A(b) genetic background have normal CD4(+) T cell populations and normal surface expression of MHC class II, but their B cell development is arrested while the cells are still immature. After a CNS injury, these mice recover better than their matched wild-type controls. We generated p41-transgenic mice on an I-A(d) background (p41-I-A(d) mice), and found that their recovery from CNS injuries was worse than that of controls. A correlative inverse effect was seen with respect to the kinetics of T cell and B cell recruitment to the injured CNS and the expression of insulin-like growth factor at the lesion site. These results, besides verifying previous findings that B cells function in the damaged CNS, demonstrate that the outcome of a particular genetic manipulation may be strain dependent.
authors.affiliation: Department of Neurobiology, The Weizmann Institute of Science, 76100 Rehovot, Israel.
authors.forename: Hadas
authors.initials: H
authors.lastname: Schori
authors.forename: Ravid
authors.initials: R
authors.lastname: Shechter
authors.forename: Idit
authors.initials: I
authors.lastname: Shachar
authors.forename: Michal
authors.initials: M
authors.lastname: Schwartz
mesh_terms: D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte / Q000235:genetics*; D001402:B-Lymphocytes / Q000276:immunology*; D002490:Central Nervous System / Q000293:injuries* / Q000473:pathology; D002493:Central Nervous System Diseases / Q000235:genetics* / Q000276:immunology* / Q000473:pathology; D006239:Haplotypes; D000949:Histocompatibility Antigens Class II / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic; D020033:Protein Isoforms / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000944:Antigens, Differentiation, B-Lymphocyte; D000949:Histocompatibility Antigens Class II; C474482:I-A(b) antigen, mouse; C111389:I-Ad antigen; D020033:Protein Isoforms; C045084:invariant chain