title: The role of actin, actomyosin and microtubules in defining cell shape during the differentiation of Naegleria amebae into flagellates.
abstract: Differentiation of Naegleria amebae into flagellates was used to examine the interaction between actin, actomyosin and microtubules in defining cell shape. Amebae, which lack microtubules except during mitosis, differentiate into flagellates with a fixed shape and a complex microtubule cytoskeleton in 120 min. Based on earlier models of ameboid motility it has been suggested that actomyosin is quiescent in flagellates. This hypothesis was tested by following changes in the cytoskeleton using three-dimensional reconstructions prepared by confocal microscopy of individual cells stained with antibodies against actin and tubulin as well as with phalloidin and DNase I. F-actin as defined by phalloidin staining was concentrated in expanding pseudopods. Most phalloidin staining was lost as cells rounded up before the onset of flagellum formation. Actin staining with a Naegleria-specific antibody that recognizes both F- and G-actin was confined to the cell cortex of both amebae and flagellates. DNase I demonstrated G-actin throughout all stages. Most of the actin in the cortex was not bound by phalloidin yet was resistant to detergent extraction suggesting that it was polymerized. The microtubule cytoskeleton of flagellates was intimately associated with this actin cortex. Treatment of flagellates with cytochalasin D produced a rapid loss of flagellate shape and the appearance of phalloidin staining while latrunculin A stabilized the flagellate shape. These results suggest that tension produced by an actomyosin network is required to maintain the flagellate shape. The rapid loss of the flagellate shape induced by drugs, which specifically block myosin light chain kinase, supports this hypothesis.
authors.affiliation: Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA. cwalsh@pitt.edu
authors.forename: Charles J
authors.initials: CJ
authors.lastname: Walsh
mesh_terms: D000199:Actins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000205:Actomyosin / Q000494:pharmacology; D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D048430:Cell Shape / Q000502:physiology*; D015638:Cytochalasin D / Q000494:pharmacology; D003599:Cytoskeleton / Q000502:physiology; D003850:Deoxyribonuclease I / Q000494:pharmacology; D005407:Flagella / Q000502:physiology*; D007700:Kinetics; D018613:Microscopy, Confocal; D008870:Microtubules / Q000502:physiology*; D009253:Naegleria / Q000166:cytology*; D015739:Nocodazole / Q000494:pharmacology; D010590:Phalloidine / Q000494:pharmacology; D013045:Species Specificity; D053778:Thiazolidines / Q000494:pharmacology; D014404:Tubulin / Q000032:analysis / Q000378:metabolism
chemical_list: D000199:Actins; D019086:Bridged Bicyclo Compounds, Heterocyclic; D053778:Thiazolidines; D014404:Tubulin; D010590:Phalloidine; D015638:Cytochalasin D; D000205:Actomyosin; D003850:Deoxyribonuclease I; D015739:Nocodazole; C037067:latrunculin A