[Quality analysis and evaluation of Rhizoma Coptidis under different cultivation conditions].
OBJECTIVE
To develop methods for the fingerprint analysis of Rhizoma Coptidis and the determination of berberine, palmatine and jatrorrhizine in Rhizoma Coptidis, and analyze the contents of these three alkaloids in Rhizoma Coptidis under different cultivation conditions, from different areas and processed with different methods.


METHODS
Two methods (HPLC-UV and HPLC-MS) have been developed and used in fingerprint analysis of Rhizoma Coptidis. An HPLC method was used to determine the contents of three alkaloids.


RESULTS
With HPLC-MS techniques, seven major chromatographic peaks in the fingerprint analysis of Rhizoma Coptidis were identified by their MS spectra and compared with the reference standards. In different cultivation conditions, shading conditions and growing ages have obvious influence on the contents of three alkaloids in Rhizoma Coptidis, while planting density was not the major factor that influenced the contents of three alkaloids. The contents of three alkaloids of Coptidis samples were almost higher than those of Coptidis reference material. For Coptidis samples from different cultivation area, the contents of these three alkaloids were different greatly. For Coptidis samples processed with different methods, the contents of three alkaloids were not influenced obviously by processing methods.


CONCLUSIONS
The results showed that the ecology cultivation method to replace the traditional shading method was feasible and provided the theoretical foundation for scientifically processing Rhizoma Coptidis.
Sheng
Yu-Xin
YX
Institute of Materia Media, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Zhang
Jin-Lan
JL
Sun
Su-Qin
SQ
Xu
Jin-Tang
JT
D001599:Berberine / Q000031:analogs & derivatives* / Q000032:analysis* / Q000592:standards; D001600:Berberine Alkaloids / Q000032:analysis*; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D039323:Coptis / Q000737:chemistry* / Q000254:growth & development; D017753:Ecosystem; D010946:Plants, Medicinal / Q000737:chemistry / Q000254:growth & development; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry / Q000254:growth & development; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
D001600:Berberine Alkaloids; C055785:jatrorrhizine; D001599:Berberine; C005413:palmatine