The molecular retention mechanism in reversed-phase liquid chromatography of meso-ionic compounds by quantitative structure-retention relationships (QSRR).
Absorption, distribution, metabolism, and excretion (ADME) properties are of invaluable importance to a bioactive compound. Permeation process is one of the most widely studied by many different techniques. Among them, reversed-phase liquid chromatography (RP-LC) is proving to be of great interest, in which retention time can provide some insights on the hydrophobic interaction behavior. A series of antiprotozoal meso-ionic 1,3,4-thiadiazolium-3-aminides were studied in order to obtain the retention-time data through two different organic modifiers using a commercially available Supelcosil ABZ(+) Plus column. MeOH/buffer mobile phase provided the best results in assessing the hydrophobicity of these compounds as compared to MeCN. Quantitative Structure-Retention Relationships (QSRR) models were built to identify the physicochemical properties that govern the process by using PLS analysis. VolSurf descriptors extracted from water (H(2)O), hydrophobic (DRY), amide N-atom (N(1)) and carbonyl O-atom (O) probes showed that the retention is positively correlated to the molecules size and shape, hydrophobic pattern, and dispersion forces. Hydrogen bonding depicts the solvent force considering the donor and acceptor differences between MeOH and MeCN organic modifiers.
Almeida
Tânia M G
TM
Núcleo de Estudos em Química Medicinal--NEQUIM--Departamento de Química, Universidade Federal de Minas Gerais, Campus Pampulha, 31270-901, Belo Horizonte, MG, Brazil.
Leitão
Andrei
A
Montanari
Maria Luiza C
ML
Montanari
Carlos A
CA
D000981:Antiprotozoal Agents / Q000032:analysis* / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical / Q000295:instrumentation / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods; D021281:Quantitative Structure-Activity Relationship
D000981:Antiprotozoal Agents