[Effects of ganoderma lucidum spores on cytochrome C and mitochondrial calcium in the testis of NIDDM rats].
OBJECTIVE
To observe the effects of Ganoderma lucidum spores on Cytochrome C (Cyt-C) and mitochondrial calcium in the testis of NIDDM rats.


METHODS
Fifty male Wistar rats were divided randomly into three groups: model, ganoderma and normal control, the first two groups injected with 2% STZ through vena caudalis, and the last one with half-and-half sodium citrate/citrate buffer solution. Two weeks after normal diet, glucose tolerance tests were performed and the rats with abnormal glucose tolerance from the model and ganoderma groups received high-fat and high-carbohydrate food, the ganoderma group given Ganoderma lucidum spores (250mg/[ kg x d] ) in addition, both for 10 weeks. Glucose tolerance tests were repeated 1 day before the end of the experiment and the rats were castrated and relevant indexes measured.


RESULTS
The NIDDM model was successfully constructed. In the model group, the levels of mitochondrial Cyt-C and mitochondrial calcium were significantly lower (P <0. 05) while that of the plasma Cyt-C was significantly higher than in the ganoderma and the control groups.


CONCLUSIONS
Cyt-C and calcium ion are involved in the damage of the testis. Ganoderma lucidum spores can protect the testis of NIDDM rats.
Wang
Bai-xin
BX
Department of Pathology and Physiology, College of Basic Medical Sciences, China.
Wang
Shu-qiu
SQ
Qin
Wen-bo
WB
Wang
Shu-xiang
SX
Ma
Xiao-ru
XR
Zhang
Ting
T
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D045304:Cytochromes c / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy*; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D020940:Reishi; D013737:Testis / Q000187:drug effects / Q000378:metabolism*
D045304:Cytochromes c; D002118:Calcium