title: Molecular and functional characterization of a cyclophilin with antifungal activity from Chinese cabbage.
abstract: An antifungal protein that inhibits the growth of filamentous fungal pathogens was isolated from Chinese cabbage (Brassica campestris L. ssp. pekinensis) by affinity chromatography on Affi-gel blue gel and ion exchange chromatography on CM-Sepharose. The N-terminal amino acid sequence of the protein was highly homologous to that of plant cyclophilins and consequently the protein was denoted as C-CyP. To understand the antifungal activity of C-CyP, we isolated a cDNA encoding its gene from a Chinese cabbage leaf cDNA library. The Chinese cabbage genome bears more than one C-CyP gene copy and C-CyP mRNA is highly expressed in all tissues except the seeds. Recombinant C-CyP catalyzed the cis-trans inter-conversion of the Ala-Pro bond of the substrate, which indicates this protein has peptidyl-prolyl cis-trans isomerase activity. It also inhibited the growth of several fungal pathogens.
authors.affiliation: Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju, 660-701, Republic of Korea.
authors.forename: Jung Ro
authors.initials: JR
authors.lastname: Lee
authors.forename: Seong-Cheol
authors.initials: SC
authors.lastname: Park
authors.forename: Jin-Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Seung Sik
authors.initials: SS
authors.lastname: Lee
authors.forename: Yoonkyung
authors.initials: Y
authors.lastname: Park
authors.forename: Gang-Won
authors.initials: GW
authors.lastname: Cheong
authors.forename: Kyung-Soo
authors.initials: KS
authors.lastname: Hahm
authors.forename: Sang Yeol
authors.initials: SY
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification; D001483:Base Sequence; D020171:Botrytis / Q000187:drug effects; D001937:Brassica / Q000737:chemistry*; D002176:Candida albicans / Q000187:drug effects; D021983:Cyclophilins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D004926:Escherichia coli / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005670:Fusarium / Q000187:drug effects; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000494:pharmacology; D012232:Rhizoctonia / Q000187:drug effects; D016415:Sequence Alignment
chemical_list: D000935:Antifungal Agents; D011994:Recombinant Proteins; D021983:Cyclophilins; D016650:Fluorescein-5-isothiocyanate