Effects of sinusoidal electromagnetic field on structure and function of different kinds of cell lines.
This study investigated that whether a 2 mT, 60 Hz, sinusoidal electromagnetic field (EMF) alters the structure and function of cells. This research compared the effects of EMF on four kinds of cell lines: hFOB 1.19 (fetal osteoblast), T/G HA-VSMC (aortic vascular smooth muscle cell), RPMI 7666 (B lymphoblast), and HCN-2 (cortical neuronal cell). Over 14 days, cells were exposed to EMF for 1, 3, or 6 hours per day (hrs/d). The results pointed to a cell type-specific reaction to EMF exposure. In addition, the cellular responses were dependent on duration of EMF exposure. In the present study, cell proliferation was the trait most sensitive to EMF. EMF treatment promoted growth of hFOB 1.19 and HCN-2 compared with control cells at 7 and 14 days of incubation. When the exposure time was 3 hrs/d, EMF enhanced the proliferation of RPMI 7666 but inhibited that of T/G HA- VSMC. On the other hand, the effects of EMF on cell cycle distribution, cell differentiation, and actin distribution were unclear. Furthermore, we hardly found any correlation between EMF exposure and gap junctional intercellular communication in hFOB 1.19. This study revealed that EMF might serve as a potential tool for manipulating cell proliferation.
Sul
Ah Ram
AR
Department of Medical Engineering, Yonsei University College of Medicine, Seoul, Korea.
Park
Si-Nae
SN
Suh
Hwal
H
D008841:Actin Cytoskeleton / Q000528:radiation effects; D002453:Cell Cycle / Q000528:radiation effects; D002454:Cell Differentiation / Q000528:radiation effects; D002460:Cell Line; D002468:Cell Physiological Phenomena / Q000528:radiation effects*; D049109:Cell Proliferation / Q000528:radiation effects; D004574:Electromagnetic Fields; D017629:Gap Junctions / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D015398:Signal Transduction