title: Proteomic comparison of Mycobacterium avium subspecies paratuberculosis grown in vitro and isolated from clinical cases of ovine paratuberculosis.
abstract: Paratuberculosis (Johne's disease) poses a significant economic problem to beef, dairy and sheep industries worldwide, and is caused by Mycobacterium avium subspecies paratuberculosis. In this study, 2D PAGE was used as a tool to investigate the virulent state of M. avium subsp. paratuberculosis, incorporating the technique of beating the organism with zirconium/silica beads to provide a comprehensive representation of its proteome. A direct comparison of the proteomes of M. avium subsp. paratuberculosis scraped from the terminal ileum of ovine paratuberculosis cases, and the identical strain grown in vitro, is presented. These analyses identified a set of 10 proteins whose expression is upregulated during natural infection: 1-pyrroline-5-carboxylate dehydrogenase (RocA), a putative acyl-CoA dehydrogenase (FadE14), 2-methylcitrate dehydratase (2-mcd), arginosuccinate synthase (ArgG), universal stress protein (usp), 30S ribosomal protein S2 (RpsB), peptidyl-prolyl cis-trans isomerase (PpiA), luciferase-like monooxygenase (lmo), thiosulfate sulfurtransferase (SseA) and ATP-dependent Clp protease (ClpB). Most of the proteins identified do not have obviously related functions; however, ArgG and RocA function in the same pathway, and may have a concerted action for energy production in vivo.
authors.affiliation: Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik EH26 0PZ, UK. Val.Hughes@moredun.ac.uk
authors.forename: Valerie
authors.initials: V
authors.lastname: Hughes
authors.forename: Stuart
authors.initials: S
authors.lastname: Smith
authors.forename: Alfredo
authors.initials: A
authors.lastname: Garcia-Sanchez
authors.forename: Jill
authors.initials: J
authors.lastname: Sales
authors.forename: Karen
authors.initials: K
authors.lastname: Stevenson
mesh_terms: D050842:1-Pyrroline-5-Carboxylate Dehydrogenase / Q000378:metabolism; D000818:Animals; D001124:Argininosuccinate Synthase / Q000378:metabolism; D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism*; D029968:Escherichia coli Proteins; D007082:Ileum / Q000382:microbiology; D016927:Mycobacterium avium subsp. paratuberculosis / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D010283:Paratuberculosis / Q000382:microbiology*; D040901:Proteomics; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*; D015854:Up-Regulation; D015040:Zirconium / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D015040:Zirconium; D050842:1-Pyrroline-5-Carboxylate Dehydrogenase; C474647:ArgG protein, E coli; D001124:Argininosuccinate Synthase