title: Misexpression screen in Drosophila melanogaster aiming to reveal novel factors involved in formation of body parts.
abstract: To identify novel factors that lead a fly imaginal disc to adopt its developmental fate, we carried out a modular dominant misexpression screen in imaginal discs. We have identified two factors that appear to change the fate of the respective body structure and appear to lead to the transformation of a body part. In one mutant line, notum tissue, normally derived from wing imaginal tissue, formed close to the site of the sternopleural bristles, which are leg disc derivatives. In the other line, the arista is transformed into a tubular structure, resembling an abnormal leg. We found that ectopic expression of abrupt was responsible for this potential transformation of the arista.
authors.affiliation: Biozentrum der Universität Basel, Abteilung Zellbiologie, CH-4056 Basel, Switzerland. nicole.grieder@unibas.ch
authors.forename: Nicole C
authors.initials: NC
authors.lastname: Grieder
authors.forename: Ilias
authors.initials: I
authors.lastname: Charlafti
authors.forename: Urs
authors.initials: U
authors.lastname: Kloter
authors.forename: Herbert
authors.initials: H
authors.lastname: Jäckle
authors.forename: Ulrich
authors.initials: U
authors.lastname: Schäfer
authors.forename: Walter J
authors.initials: WJ
authors.lastname: Gehring
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D019521:Body Patterning / Q000235:genetics; D003433:Crosses, Genetic; D004247:DNA / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development*; D005121:Extremities / Q000254:growth & development; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D014921:Wings, Animal / Q000254:growth & development
chemical_list: D004247:DNA