title: Growth inhibitory effects of gastric cancer cells with an increase in S phase and alkaline phosphatase activity repression by aloe-emodin.
abstract: Aloe-emodin is a novel active compound found in the root and rhizome of Rheum palmatum. To investigate the effects and mechanisms of aloe-emodin on human gastric cancer, MGC-803 cells were treated with 2.5, 5, 10, 20 and 40 microM aloe-emodin for 1-5 d. The results showed that aloe-emodin inhibited the growth of cancer cells in a dose-dependent manner with an increase in S phase and in the proportion of cells cycling at a higher ploidy level (>G2/M). Moreover, the alkaline phosphatase (ALP) activity, an indicator of cell differentiation, was found decreased. This is one of the first to focus on the effect of ALP activity in human gastric carcinomas cells treated by aloe-emodin. These results indicate that aloe-emodin has a potential value for the treatment of gastric cancer and its mechanisms are by means of cell cycle interruption and induce differentiation.
authors.affiliation: School of Medicine, Ningbo University, Ningbo, China. junmingguo@yahoo.com
authors.forename: Junming
authors.initials: J
authors.lastname: Guo
authors.forename: Bingxiu
authors.initials: B
authors.lastname: Xiao
authors.forename: Shun
authors.initials: S
authors.lastname: Zhang
authors.forename: Donghai
authors.initials: D
authors.lastname: Liu
authors.forename: Yiping
authors.initials: Y
authors.lastname: Liao
authors.forename: Qian
authors.initials: Q
authors.lastname: Sun
mesh_terms: D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors*; D000880:Anthraquinones; D049109:Cell Proliferation / Q000187:drug effects; D004642:Emodin / Q000494:pharmacology*; D006801:Humans; D012250:Rheum / Q000737:chemistry; D016196:S Phase / Q000187:drug effects; D013274:Stomach Neoplasms / Q000201:enzymology*; D014407:Tumor Cells, Cultured
chemical_list: D000880:Anthraquinones; C518327:aloe emodin; D000469:Alkaline Phosphatase; D004642:Emodin