Synthesis and applications of oligonucleotide-carbohydrate conjugates.
Nowadays, oligonucleotide-carbohydrate conjugates are used in antisense biotechnology and in the study of glycosylated DNA functioning in vitro. The application of mono- and disaccharide phosphoramidites, solid-phase supports with immobilized carbohydrates, glycosylated nucleoside phosphoramidites, and postsynthetic conjugation of reactive sugar derivatives with oligonucleotides for preparation of oligonucleotide-carbohydrate conjugates have been systematically studied. The advantages and disadvantages of these approaches are considered. Possible strategies for synthesis of glycoclusters with different topologies conjugated to DNA are discussed. Applications of oligonucleotide-carbohydrate conjugates are highlighted. Studies of interactions of glycosylated oligonucleotides with proteins and effective cell-specific delivery of oligonucleotide-carbohydrate conjugates are discussed.
Zatsepin
Timofei S
TS
Department of Chemistry, M. V. Lomonosov Moscow State University, Leninskie Gory, Moscow, Russia, 119992.
Oretskaya
Tatiana S
TS
D002241:Carbohydrates / Q000008:administration & dosage / Q000138:chemical synthesis*; D004247:DNA / Q000008:administration & dosage / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D006031:Glycosylation; D009841:Oligonucleotides / Q000008:administration & dosage / Q000138:chemical synthesis*
D002241:Carbohydrates; D009841:Oligonucleotides; D004247:DNA