title: Enrichment of phosphotyrosine proteome of human platelets by immunoprecipitation.
abstract: Proteomics offers the opportunity to comprehensively investigate the anucleate platelet. Here, we present a detailed procedure for enrichment by immunoprecipitation, using the monoclonal antibody 4G10, of the dynamic phosphotyrosine proteome of human platelets. Such an approach offers the possibility of capturing the dynamic tyrosine phosphorylation events that occur upon platelet activation and aggregation, with an aim to identify novel signaling proteins.
authors.affiliation: Department of Clinical Pharmacology, Royal College of Surgeons's in Ireland, Dublin, Ireland.
authors.forename: Martina
authors.initials: M
authors.lastname: Foy
authors.forename: Donal F
authors.initials: DF
authors.lastname: Harney
authors.forename: Kieran
authors.initials: K
authors.lastname: Wynne
authors.forename: Patricia B
authors.initials: PB
authors.lastname: Maguire
mesh_terms: D001792:Blood Platelets / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods*; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000378:metabolism*; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics / Q000379:methods
chemical_list: D020543:Proteome; D019000:Phosphotyrosine