Lack of the PAC1 receptor alters the circadian expression of VIP mRNA in the suprachiasmatic nucleus of mice.
PACAP in the retinohypothalamic tract mediates photic information to the suprachiasmatic nucleus via the PAC1 receptor. The diurnal and circadian VIP mRNA expressions in the suprachiasmatic nucleus of PAC1-/- and wild type mice were quantified. During light/dark cycles identical VIP mRNA rhythms were found while the oscillation pattern differed between the two types of animals during constant darkness. The results show that the circadian VIP mRNA expression is influenced by the absence of PAC1 signalling.
Georg
Birgitte
B
Department of Clinical Biochemistry, Bispebjerg University Hospital, DK-2400 Copenhagen NV, Denmark. bg06@bbh.hosp.dk
Hannibal
Jens
J
Fahrenkrug
Jan
J
D000818:Animals; D002940:Circadian Rhythm / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D012333:RNA, Messenger / Q000378:metabolism; D051237:Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I / Q000172:deficiency*; D013493:Suprachiasmatic Nucleus / Q000378:metabolism*; D014660:Vasoactive Intestinal Peptide / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; D051237:Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I; D014660:Vasoactive Intestinal Peptide