Statistical optimization of medium components for avilamycin production by Streptomyces viridochromogenes Tü57-1 using response surface methodology.
A fermentation medium for avilamycin production by Streptomyces viridochromogenes Tü57-1 has been optimized. Important components and their concentrations were investigated using fractional factorial design and Box-Behnken Design. The results showed that soybean flour, soluble starch, MgSO4.7H2O and CaCl2.2H2O are important for avilamycin production. A polynomial model related to medium components and avilamycin yield had been established. A high coefficient of determination (R2 = 0.92) was obtained that indicated good agreement between the experimental and predicted values of avilamycin yield. Student's T-test of each coefficient showed that all the linear and quadratic terms had significant effect (P > |T| < 0.05) on avilamycin yield. The significance of tested components was related to MgSO4.7H2O (0.37 g/L), CaCl2.2H2O (0.39 g/L), soybean flour (21.97 g/L) and soluble starch (37.22 g/L). The yield of avilamycin reached 88.33 +/- 0.94 mg/L (p < 0.05) that was 2.8-fold the initial yield.
Zhu
Chuan-He
CH
College of Food Science and Engineering, Shangdong Agriculture University, Taian 271018, China.
Lu
Fu-Ping
FP
He
Ya-Nan
YN
Zhang
Juan-Kun
JK
Du
Lian-Xiang
LX
D018533:Biomass; D002244:Carbon / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism* / Q000494:pharmacology; D005285:Fermentation / Q000187:drug effects; D007218:Industrial Microbiology / Q000379:methods; D009584:Nitrogen / Q000378:metabolism; D009844:Oligosaccharides / Q000096:biosynthesis*; D015203:Reproducibility of Results; D013302:Streptomyces / Q000254:growth & development / Q000378:metabolism*
D003470:Culture Media; D009844:Oligosaccharides; C006653:avilamycin; D002244:Carbon; D009584:Nitrogen