title: [Preliminary data concerning cross-reacting anticardiolipin antibodies as potential inhibiting endotoxin activity factors].
abstract: In analyzed synovial fluids, suspected for endotoxin presence, the presence of endotoxin done was proved by LAL-test in 21 synovial fluids /42%/ and in 12 of 20 /60%/ the presence of endotoxin inhibiting substances /IS/ was proved. Because many parameters /like presence CIC, anti-LPS, KDO/ indicates that in the group of LAL-negative /IS/ - positive synovial fluids the presence of endotoxin is very possible, IS have been removed /by heating to 100 degrees C/ and after that the endotoxin activity was shown in about 27% of synovial fluids. Additional analysis of the Cohn fraction /prepared by ammonium sulfate precipitation/ confirmed the presence in the gamma and alpha and beta fractions a weak IS activity /in a dose dependent manner. No IS activity, have been shown yet in the fraction of affinity purified aCl antibodies.
authors.affiliation: Instytut Reumatologii im. prof. dr hab. med. Eleonory Reicher w Warszawie, Zakład Mikrobiologii i Serologii.
authors.forename: Jakub
authors.initials: J
authors.lastname: Zabek
authors.forename: Jacek
authors.initials: J
authors.lastname: Noworyta
authors.forename: Maria
authors.initials: M
authors.lastname: Brasse-Rumin
authors.forename: Joanna
authors.initials: J
authors.lastname: Pyka
authors.forename: Iwona
authors.initials: I
authors.lastname: Krzewska
authors.forename: Maria
authors.initials: M
authors.lastname: Rell-Bakalarska
mesh_terms: D017153:Antibodies, Anticardiolipin / Q000032:analysis / Q000276:immunology*; D000907:Antibodies, Bacterial / Q000032:analysis / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology; D001168:Arthritis / Q000276:immunology*; D001172:Arthritis, Rheumatoid / Q000276:immunology / Q000382:microbiology; D003429:Cross Reactions; D006801:Humans; D008033:Limulus Test / Q000379:methods; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000276:immunology*; D010003:Osteoarthritis / Q000276:immunology / Q000382:microbiology; D025241:Spondylarthritis / Q000276:immunology / Q000382:microbiology; D013582:Synovial Fluid / Q000276:immunology*
chemical_list: D017153:Antibodies, Anticardiolipin; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D008070:Lipopolysaccharides