title: Solving multicomponent chiral separation challenges using a new SFC tandem column screening tool.
abstract: A tool for improved tandem column chiral supercritical fluid chromatography (SFC) method development screening was prepared by modification of a commercial analytical SFC instrument with two different software-controllable, six position high-pressure column selection valves, each controlling a bank of five different columns and a pass through line. The resulting instrument, which has the ability to screen 10 different individual columns and 25 different tandem column arrangements, is a useful tool for facilitating the screening of tandem column SFC arrangements for separation of complex mixtures of stereoisomers or other multicomponent mixtures. Strategies for optimal use of the instrument are discussed, and several examples of the use of the instrument in developing tandem SFC methods for resolution of multicomponent mixtures are presented.
authors.affiliation: Department of Process Research, Separation and Analysis Technologies, Merck Research Laboratories, RY 801-C101, Rahway, New Jersey 07065, USA. christopher_welch@merck.com
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Welch
authors.forename: Mirlinda
authors.initials: M
authors.lastname: Biba
authors.forename: Joseph R
authors.initials: JR
authors.lastname: Gouker
authors.forename: Gary
authors.initials: G
authors.lastname: Kath
authors.forename: Paul
authors.initials: P
authors.lastname: Augustine
authors.forename: Paul
authors.initials: P
authors.lastname: Hosek
mesh_terms: D025924:Chromatography, Supercritical Fluid / Q000295:instrumentation*; D013237:Stereoisomerism