Methylcytosine-selective fluorescence quenching by osmium complexation.
We report on the control of the emission from a fluorophore fixed on DNA using the methylcytosine-selective addition of an osmium-bipyridine complex. We have synthesized DNA modified by a microenvironment-sensitive fluorophore, 2-dimethylamino-6-acyl-naphthalene. The emission from the fluorophore tethered to a probe DNA was effectively quenched by a methylcytosine glycol-osmium-bipyridine triad, which was located in the immediate neighborhood of the fluorophore. The discrimination of the cytosine methylation status at a methylation hot spot in the p53 gene was also executed using a well-designed fluorescent DNA probe.
Tanaka
Kazuo
K
Frontier Research System, RIKEN (The Institute of Physical and Chemical Research), Wako, Saitama 351-0198, Japan.
Tainaka
Kazuki
K
Okamoto
Akimitsu
A
D000818:Animals; D003596:Cytosine / Q000031:analogs & derivatives / Q000032:analysis*; D019175:DNA Methylation; D015342:DNA Probes; D005453:Fluorescence; D016158:Genes, p53 / Q000235:genetics; D006801:Humans; D008745:Methylation; D009992:Osmium
D015342:DNA Probes; D009992:Osmium; D003596:Cytosine