title: 3D7-Derived Plasmodium falciparum erythrocyte membrane protein 1 is a frequent target of naturally acquired antibodies recognizing protein domains in a particular pattern independent of malaria transmission intensity.
abstract: Protection against Plasmodium falciparum malaria is largely mediated by IgG against surface Ags such as the erythrocyte membrane protein 1 family (PfEMP1) responsible for antigenic variation and sequestration of infected erythrocytes. PfEMP1 molecules can be divided into groups A, B/A, B, C, and B/C. We have previously suggested that expression of groups A and B/A PfEMP1 is associated with severe disease and that Abs to these molecules are acquired earlier in life than Abs to PfEMP1 belonging to groups B, B/C, and C PfEMP1. In this study, we compared the acquisition of IgG to 20 rPfEMP1 domains derived from 3D7 in individuals living under markedly different malaria transmission intensity and were unable to find differences in the Ab acquisition rate to PfEMP1 of different groupings (A, B, or C) or domain type (alpha, beta, gamma, delta, epsilon, or x). Abs were acquired early in life in individuals living in the high transmission village and by the age of 2-4 years most individuals had Abs against most constructs. This level of reactivity was found at the age of 10-20 years in the medium transmission village and was never reached by individuals living under low transmission. Nevertheless, the sequence by which individuals acquired Abs to particular constructs was largely the same in the three villages. This indicates that the pattern of PfEMP1 expression by parasites transmitted at the different sites was similar, suggesting that PfEMP1 expression is nonrandom and shaped by host-parasite relationship factors operating at all transmission intensities.
authors.affiliation: Centre for Medical Parasitology, Department of Medical Microbiology and Immunology, University of Copenhagen and Copenhagen University Hospital, Copenhagen, Denmark.
authors.forename: Louise
authors.initials: L
authors.lastname: Joergensen
authors.forename: Lasse S
authors.initials: LS
authors.lastname: Vestergaard
authors.forename: Louise
authors.initials: L
authors.lastname: Turner
authors.forename: Pamela
authors.initials: P
authors.lastname: Magistrado
authors.forename: John P
authors.initials: JP
authors.lastname: Lusingu
authors.forename: Martha
authors.initials: M
authors.lastname: Lemnge
authors.forename: Thor G
authors.initials: TG
authors.lastname: Theander
authors.forename: Anja T R
authors.initials: AT
authors.lastname: Jensen
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood / Q000276:immunology*; D000918:Antibody Specificity; D000940:Antigenic Variation / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006790:Host-Parasite Interactions; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D016778:Malaria, Falciparum / Q000276:immunology* / Q000635:transmission; D008297:Male; D010963:Plasmodium falciparum / Q000276:immunology*; D017434:Protein Structure, Tertiary; D015800:Protozoan Proteins / Q000276:immunology*; D013636:Tanzania
chemical_list: D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D015800:Protozoan Proteins; C085731:erythrocyte membrane protein 1, Plasmodium falciparum