Biological responses of ligament fibroblasts and gene expression profiling on micropatterned silicone substrates subjected to mechanical stimuli.
In this study, ligament fibroblasts were cultivated on micropatterned silicone substrates and subjected to cyclic stretching to simulate the in vivo biomechanical environment during ligament healing. Without stretching, ligament fibroblasts were aligned parallel to the microgrooves on the silicone substrate surface. However, we previously reported that uniaxial cyclic stretching induces alignment perpendicular to the stretching axis. With stretching on a microgrooved surface, cell proliferation and collagen production were greatly enhanced. The exact functions of the micropatterned surface and mechanical stimuli are unknown. Therefore, in gene expression microarray experiments, genes whose expression is inhibited by subculture from passage 0 (P0) to passage 8 (P8) and enhanced by micropatterning and stretching were sought out. The following six genes were selected: MGP, GADD45A, UNC5B, TGFB1, COL4A1, and COL4A2. The selected genes play fundamental roles in cell proliferation, differentiation, apoptosis, and structural maintenance. On the basis of the obtained gene expression profiles, we identified candidate genes that might be involved in responses to a micropatterned surface and mechanical stretching.
Park
Su A
SA
Department of Future Technology, Korea Institute of Machinery & Materials, Jang-dong, Yuseong-gu, Daejeon 305-343, Korea.
Kim
In Ae
IA
Lee
Yong Jae
YJ
Shin
Ji Won
JW
Kim
Chong-Rak
CR
Kim
Jeong Koo
JK
Yang
Young-Il
YI
Shin
Jung-Woog
JW
D016118:Anterior Cruciate Ligament / Q000166:cytology* / Q000502:physiology; D016764:Cell Polarity; D049109:Cell Proliferation; D002478:Cells, Cultured; D005347:Fibroblasts / Q000502:physiology*; D020869:Gene Expression Profiling; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D012828:Silicones; D013314:Stress, Mechanical; D023822:Tissue Engineering
D012828:Silicones