Artificially engineered magnetic nanoparticles for ultra-sensitive molecular imaging.
Successful development of ultra-sensitive molecular imaging nanoprobes for the detection of targeted biological objects is a challenging task. Although magnetic nanoprobes have the potential to perform such a role, the results from probes that are currently available have been far from optimal. Here we used artificial engineering approaches to develop innovative magnetic nanoprobes, through a process that involved the systematic evaluation of the magnetic spin, size and type of spinel metal ferrites. These magnetism-engineered iron oxide (MEIO) nanoprobes, when conjugated with antibodies, showed enhanced magnetic resonance imaging (MRI) sensitivity for the detection of cancer markers compared with probes currently available. Also, we successfully visualized small tumors implanted in a mouse. Such high-performance, nanotechnology-based molecular probes could enhance the ability to visualize other biological events critical to diagnostics and therapeutics.
Lee
Jae-Hyun
JH
Department of Chemistry and Nanomedical National Core Research Center, Yonsei University, Seoul 120-749, Korea.
Huh
Yong-Min
YM
Jun
Young-wook
YW
Seo
Jung-wook
JW
Jang
Jung-tak
JT
Song
Ho-Taek
HT
Kim
Sungjun
S
Cho
Eun-Jin
EJ
Yoon
Ho-Geun
HG
Suh
Jin-Suck
JS
Cheon
Jinwoo
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D061067:Antibodies, Monoclonal, Humanized; D014408:Biomarkers, Tumor / Q000032:analysis; D002460:Cell Line; D045744:Cell Line, Tumor; D005260:Female; D005290:Ferric Compounds / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008280:Magnetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology; D009374:Neoplasms, Experimental / Q000175:diagnosis / Q000378:metabolism; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis / Q000276:immunology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D000068878:Trastuzumab
D000911:Antibodies, Monoclonal; D061067:Antibodies, Monoclonal, Humanized; D014408:Biomarkers, Tumor; D005290:Ferric Compounds; D018719:Erb-b2 Receptor Tyrosine Kinases; D000068878:Trastuzumab; C000499:ferric oxide