title: The ameliorating effect of oroxylin A on scopolamine-induced memory impairment in mice.
abstract: Oroxylin A is a flavonoid and was originally isolated from the root of Scutellaria baicalensis Georgi., one of the most important medicinal herbs in traditional Chinese medicine. The aim of this study was to investigate the ameliorating effects of oroxylin A on memory impairment using the passive avoidance test, the Y-maze test, and the Morris water maze test in mice. Drug-induced amnesia was induced by administering scopolamine (1 mg/kg, i.p.) or diazepam (1 mg/kg, i.p.). Oroxylin A (5 mg/kg) significantly reversed cognitive impairments in mice by passive avoidance and the Y-maze testing (P<.05). Oroxylin A also improved escape latencies in training trials and increased swimming times and distances within the target zone of the Morris water maze (P<.05). Moreover, the ameliorating effects of oroxylin A were antagonized by both muscimol and diazepam (0.25 mg/kg, i.p., respectively), which are GABA(A) receptor agonists. Furthermore, oroxylin A (100 microM) was found to inhibit GABA-induced inward Cl(-) current in a single cortical neuron. These results suggest that oroxylin A may be useful for the treatment of cognitive impairments induced by cholinergic dysfunction via the GABAergic nervous system.
authors.affiliation: Department of Oriental Pharmaceutical Science and Kyung Hee East-West Pharmaceutical Research Institute, College of Pharmacy, Kyung Hee University, Dongdaemoon-Ku, Seoul, Korea.
authors.forename: Dong Hyun
authors.initials: DH
authors.lastname: Kim
authors.forename: Su Jin
authors.initials: SJ
authors.lastname: Jeon
authors.forename: Kun Ho
authors.initials: KH
authors.lastname: Son
authors.forename: Ji Wook
authors.initials: JW
authors.lastname: Jung
authors.forename: Seungjoo
authors.initials: S
authors.lastname: Lee
authors.forename: Byung Hoon
authors.initials: BH
authors.lastname: Yoon
authors.forename: Jong-Ju
authors.initials: JJ
authors.lastname: Lee
authors.forename: Young-Wuk
authors.initials: YW
authors.lastname: Cho
authors.forename: Jae Hoon
authors.initials: JH
authors.lastname: Cheong
authors.forename: Kwang Ho
authors.initials: KH
authors.lastname: Ko
authors.forename: Jong Hoon
authors.initials: JH
authors.lastname: Ryu
mesh_terms: D000647:Amnesia / Q000139:chemically induced / Q000517:prevention & control; D000704:Analysis of Variance; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects* / Q000502:physiology; D003072:Cognition Disorders / Q000188:drug therapy / Q000503:physiopathology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D005419:Flavonoids / Q000494:pharmacology*; D008297:Male; D018782:Maze Learning / Q000187:drug effects* / Q000502:physiology; D008568:Memory / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D008813:Mice, Inbred ICR; D018727:Muscarinic Antagonists; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time / Q000187:drug effects / Q000502:physiology; D011963:Receptors, GABA-A / Q000187:drug effects* / Q000502:physiology; D012601:Scopolamine; D018709:Statistics, Nonparametric
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D018727:Muscarinic Antagonists; D011963:Receptors, GABA-A; C080669:5,7-dihydroxy-6-methoxy-2-phenylchromen-4-one; D012601:Scopolamine