title: [Research of polysaccharide from Dioscorea opposita Thunb].
abstract: OBJECTIVE
  To isolate a homogeneous polysaccharide RP from RDP (a crude polysaccharide from Dioscorea opposita Thunb.) , and study its preliminary composition and structure.
  
  
  METHODS
  RP was obtained with water extraction, alcohol sedimentation, CTAB deprotein, cellulose column and SephadexG-100 column. The purities of RP were identified by SephadexG-200. PC analysis on its acidic hydrolysates was used to determine the sugar components. Sephadex chromatography was used to mensurate its molecular weight. IR was used to analysis RP.
  
  
  RESULTS
  RP was homogeneous. IR indicated that RP had beta configurations. The compositon was identified by paper chromatography as glucose, D-mannose, D-galactose.
  
  
  CONCLUSIONS
  The research could provide a theoretical foundation for further development and utilization.
authors.affiliation: Jiangsu Instiute of Botany, Chinese Academy of Sciences, Nanjing Botanical Garden, Nanjing 210014, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Xu
authors.forename: Zeng-lai
authors.initials: ZL
authors.lastname: Xu
authors.forename: Zhen-guo
authors.initials: ZG
authors.lastname: Shen
authors.forename: Yun-yun
authors.initials: YY
authors.lastname: Shi
mesh_terms: D027743:Dioscoreaceae / Q000737:chemistry*; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D008358:Mannose / Q000032:analysis; D008970:Molecular Weight; D035281:Plant Tubers / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
chemical_list: D011134:Polysaccharides; D005947:Glucose; D008358:Mannose; D005690:Galactose