title: Multifunctional polyelectrolyte multilayer films: combining mechanical resistance, biodegradability, and bioactivity.
abstract: Cross-linked polyelectrolyte multilayer films (CL PEM) have an increased rigidity and are mechanically more resistant than native (e.g., uncrosslinked) films. However, they are still biodegradable, which make them interesting candidates for biomedical applications. In this study, CL PEM films have been explored for their multifunctional properties as (i) mechanically resistant, (ii) biodegradable, and (iii) bioactive films. Toward this end, we investigated drug loading into CL chitosan/hyaluronan (CHI/HA) and poly(L-lysine)/hyaluronan (PLL/HA) films by simple diffusion of the drugs. Sodium diclofenac and paclitaxel were chosen as model drugs and were successfully loaded into the films. The effect of varying the number of layers in the (CHI/HA) films as well as the cross-linker concentration on diclofenac loading were studied. Diclofenac was released from the film in about 10 h. Paclitaxel was also found to diffuse within CL films. Its activity was maintained after loading in the CL films, and cellular viability could be reduced by about 55% over 3 days. Such a simple approach may be applied to other types of cross-linked films and to other drugs. These results prove that it is possible to design multifunctional multilayer films that combine mechanical resistance, biodegradability, and bioactivity properties into a single PEM architecture.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, Unité 595, Faculté de Chirurgie Dentaire, Université Louis Pasteur, 11 rue Humann, 67085 Strasbourg Cedex, France.
authors.forename: Aurore
authors.initials: A
authors.lastname: Schneider
authors.forename: Constant
authors.initials: C
authors.lastname: Vodouhê
authors.forename: Ludovic
authors.initials: L
authors.lastname: Richert
authors.forename: Gregory
authors.initials: G
authors.lastname: Francius
authors.forename: Erell
authors.initials: E
authors.lastname: Le Guen
authors.forename: Pierre
authors.initials: P
authors.lastname: Schaaf
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Voegel
authors.forename: Benoît
authors.initials: B
authors.lastname: Frisch
authors.forename: Catherine
authors.initials: C
authors.lastname: Picart
mesh_terms: D000327:Adsorption; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D001673:Biodegradation, Environmental; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004008:Diclofenac / Q000494:pharmacology; D004573:Electrolytes / Q000737:chemistry*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry; D046911:Macromolecular Substances / Q000737:chemistry; D018625:Microscopy, Atomic Force; D011107:Polylysine / Q000737:chemistry; D013314:Stress, Mechanical; D013718:Tensile Strength
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D003432:Cross-Linking Reagents; D004573:Electrolytes; D046911:Macromolecular Substances; D004008:Diclofenac; D011107:Polylysine; D006820:Hyaluronic Acid; D048271:Chitosan