In vivo treatment by diallyl disulfide increases histone acetylation in rat colonocytes.
Diallyl disulfide (DADS) is an organosulfur compound from garlic which exhibits various anticarcinogenic properties including inhibition of tumor cell proliferation. DADS antiproliferative effects were previously associated with an increase in histone acetylation in two human tumor colon cell lines, suggesting that DADS-induced histone hyperacetylation could be one of the mechanisms involved in its protective properties on colon carcinogenesis. The effects of DADS on histone H4 and H3 acetylation levels were investigated in vivo in colonocytes isolated from non-tumoral rat. Administrated by intracaecal perfusion or gavage, DADS increases histone H4 and H3 acetylation in colonocytes. Moreover, data generated using cDNA expression arrays suggest that DADS could modulate the expression of a subset of genes. These results suggest the involvement of histone acetylation in modulation of gene expression by DADS in normal rat colonocytes, which might play a role in its biological effects as well as in its anticarcinogenic properties in vivo.
Druesne-Pecollo
Nathalie
N
Laboratoire de Nutrition et Sécurité Alimentaire, INRA, Domaine de Vilvert, 78352 Jouy-en-Josas cedex, France. Nathalie.Pecollo@jouy.inra.fr
Chaumontet
Catherine
C
Pagniez
Anthony
A
Vaugelade
Pierre
P
Bruneau
Aurélia
A
Thomas
Muriel
M
Cherbuy
Claire
C
Duée
Pierre-Henri
PH
Martel
Paule
P
D000107:Acetylation / Q000187:drug effects; D000498:Allyl Compounds / Q000008:administration & dosage*; D000818:Animals; D002478:Cells, Cultured; D003106:Colon / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004220:Disulfides / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D004750:Enteral Nutrition; D015870:Gene Expression / Q000187:drug effects; D006657:Histones / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D020543:Proteome / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D000498:Allyl Compounds; D004220:Disulfides; D006657:Histones; D020543:Proteome; C028009:diallyl disulfide