title: Identification of ER membrane targeting signal of kinectin.
abstract: Kinectin has been identified as a kinesin receptor on endoplasmic reticulum (ER). The ER membrane binding domain of kinectin is still obscure and is thought to require a half of the molecule. To determine the ER insertion site, we produced several constructs around N-terminus of kinectin connected with green fluorescent protein (GFP) and visualized the distribution in Cos-7 cells. The fragment of residues 7-29 appeared in the reticular pattern exactly colocalized with the ER marker but did not remain for a long time. On the other hand, residues 1-106 maintained a reticular pattern for more than seven days. These results indicate that residues 7-29 of kinectin are sufficient for targeting to the ER membrane but insufficient for remaining on the ER.
authors.affiliation: Department of Neurology, Akita University School of Medicine, 1-1-1 Hondo, Akita 010-8543, Japan. erikaa@doc.med.akita-u.ac.jp
authors.forename: Erika
authors.initials: E
authors.lastname: Abe
authors.forename: Satoshi
authors.initials: S
authors.lastname: Okawa
authors.forename: Masashiro
authors.initials: M
authors.lastname: Sugawara
authors.forename: Sumio
authors.initials: S
authors.lastname: Watanabe
authors.forename: Itaru
authors.initials: I
authors.lastname: Toyoshima
mesh_terms: D000818:Animals; D001692:Biological Transport; D019556:COS Cells / Q000648:ultrastructure; D002522:Chlorocebus aethiops; D006224:Cricetinae; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D014162:Transfection / Q000379:methods
chemical_list: C088547:KTN1 protein, human; D008565:Membrane Proteins; D049452:Green Fluorescent Proteins