Dose-dependent modulation of the T cell proteome by ascorbic acid.
To investigate the hypothesis that the micronutrient ascorbic acid can modulate the functional genome, T cells (CCRF-HSB2) were treated with ascorbic acid (up to 150 microM) for up to 24 h. Protein expression changes were assessed by two-dimensional electrophoresis. Forty-one protein spots which showed greater than two-fold expression changes were subject to identification by matrix-assisted laser desorption ionisation time of flight MS. The confirmed protein identifications were clustered into five groups; proteins were associated with signalling, carbohydrate metabolism, apoptosis, transcription and immune function. The increased expression of phosphatidylinositol transfer protein (promotes intracellular signalling) within 5 min of ascorbic acid treatment was confirmed by Western blotting. Together, these observations suggest that ascorbic acid modulates the T cell proteome in a time- and dose-dependent manner and identify molecular targets for study following antioxidant supplementation in vivo.
Grant
Melissa M
MM
Aston University, Birmingham, UK.
Mistry
Nalini
N
Lunec
Joseph
J
Griffiths
Helen R
HR
D001205:Ascorbic Acid / Q000494:pharmacology*; D015153:Blotting, Western / Q000379:methods; D002461:Cell Line, Transformed; D016172:DNA Fingerprinting; D004305:Dose-Response Relationship, Drug; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D049294:Phospholipid Transfer Proteins / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects* / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013601:T-Lymphocytes / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors
D049294:Phospholipid Transfer Proteins; D020543:Proteome; D001205:Ascorbic Acid