[Improved efficient gene transfer into insect and mammalian cells by baculovirus vectors].
The fragments of genomics DNA of the nuclear polyhedrosis virus (NPV) containing genes of late viral proteins p10, p35, p39, were cloned, the promoter regions of this genes were used to design baculovirus transfer vectors. A double-promoter and triple-promoter baculovirus transfer vectors were obtained. Recombinant baculovirus vectors containing mammalian expression cassette with cytomegalovirus (CMV) promoter, the gene for green or red fluorescent protein, SV40pA and polylinker MCS were constructed for the delivery of foreign genes into mammalian cells.
Koroleva
N N
NN
Grishchuk
Iu V
IuV
Kochetkova
S V
SV
Prasolov
V S
VS
Rubtsov
P M
PM
Belzhelarskaia
S N
SN
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D003587:Cytomegalovirus / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D015246:Deoxyribonuclease EcoRI / Q000737:chemistry; D018014:Gene Transfer Techniques; D005814:Genes, Viral; D005822:Genetic Vectors / Q000235:genetics*; D007313:Insecta / Q000166:cytology*; D008164:Luminescent Proteins / Q000235:genetics; D000077183:Occlusion Body Matrix Proteins; D011401:Promoter Regions, Genetic; D014764:Viral Proteins / Q000235:genetics; D015678:Viral Structural Proteins; D000097573:Red Fluorescent Protein
D004279:DNA, Viral; D015246:Deoxyribonuclease EcoRI; D008164:Luminescent Proteins; D000077183:Occlusion Body Matrix Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; D000097573:Red Fluorescent Protein; C009829:polyhedrin protein, Nucleopolyhedrovirus