Proteomic analysis of cervical-vaginal fluid: identification of novel biomarkers for detection of intra-amniotic infection.
Intra-amniotic infection (IAI) is associated with preterm birth and perinatal mortality. To identify potential biomarkers, we performed a comprehensive survey of the cervical-vaginal fluid (CVF) proteome from a primate IAI model utilizing multidimensional protein identification technology (LC/LC-MS/MS) and MALDI-TOF-MS analyses. Analyses of CVF proteome identified 205 unique proteins and differential expression of 27 proteins in controls and IAI samples. Protein expression signatures and immunodetection of specific biomarkers identified can be employed for noninvasive detection of IAI.
Gravett
Michael G
MG
Division of Reproductive Sciences, Oregon National Primate Research Center, Beaverton, Oregon 97006, USA. gravettm@u.washington.edu
Thomas
Archana
A
Schneider
Kimberly A
KA
Reddy
Ashok P
AP
Dasari
Surendra
S
Jacob
Thomas
T
Lu
Xinfang
X
Rodland
Matthew
M
Pereira
Leonardo
L
Sadowsky
Drew W
DW
Roberts
Charles T
CT
Novy
Miles J
MJ
Nagalla
Srinivasa R
SR
D000653:Amniotic Fluid / Q000382:microbiology*; D000818:Animals; D015415:Biomarkers / Q000737:chemistry*; D001826:Body Fluids / Q000378:metabolism* / Q000382:microbiology; D002584:Cervix Uteri / Q000378:metabolism* / Q000382:microbiology; D005260:Female; D005786:Gene Expression Regulation; D007239:Infections / Q000175:diagnosis*; D008253:Macaca mulatta; D011247:Pregnancy; D011270:Pregnancy, Animal; D047928:Premature Birth / Q000517:prevention & control; D040901:Proteomics / Q000379:methods*; D014509:Ureaplasma / Q000378:metabolism; D014621:Vagina / Q000378:metabolism* / Q000382:microbiology
D015415:Biomarkers