Analysis of magnolol and honokiol in biological fluids by capillary zone electrophoresis.
Capillary zone electrophoresis (CZE) method was used for analysis of magnolol and honokiol. Under the optimized condition, CZE with UV absorption detection provided that the limit of detection was at microM level. To enhance detection sensitivity of magnolol and honokiol, CZE separation system was coupled with a laser-induced fluorescence (LIF) detector for the first time. The limits of detection of magnolol and honokiol were 12 nM (3.20 ng ml(-1)) and 18 nM (4.79 ng ml(-1)), respectively, showing that the CZE-LIF system provides greater than 100-fold sensitivity improvements than does the CZE-UV system. The developed method was applied to analyze magnolol and honokiol in spiked human plasma samples, microsome incubation samples as a preliminary demonstration of its potential in pharmacokinetic studies.
Chen
Chiu-Ling
CL
School of Pharmacy, College of Medicine, National Taiwan University, Taipei, Taiwan.
Chang
Po-Ling
PL
Lee
Shoei-Sheng
SS
Peng
Fu-Chuo
FC
Kuo
Ching-Hua
CH
Chang
Huan-Tsung
HT
D000818:Animals; D001713:Biphenyl Compounds / Q000032:analysis* / Q000097:blood; D019075:Electrophoresis, Capillary / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D017705:Lignans / Q000032:analysis* / Q000097:blood; D008862:Microsomes, Liver / Q000737:chemistry; D051381:Rats; D012015:Reference Standards; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D000498:Allyl Compounds; D010636:Phenols
D001713:Biphenyl Compounds; D017705:Lignans; C005498:magnolol; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols