title: Does chemotherapy impair the bone healing and biomechanical stability of vascularized rib and fibula grafts?
abstract: The purpose of this study was to observe the impact of chemotherapy on the healing and biomechanical properties of vascularized bone grafts. Ten male beagle dogs were divided into two experimental groups: a chemotherapy group (CH) and control group (C). Group CH received adjuvant and neo-adjuvant chemotherapy. Each animal of both groups underwent the following operative procedures. The 5th and 7th rib were removed and replaced by vascularized pedicle transfers of the adjacent 4th and 8th rib. Additionally, a free fibular flap was elevated and retransferred to the same anatomic position. The rate of bony union on plain x-ray was 100 percent in group C, 30 percent in the vascularized rib, and 80 percent in the fibula grafts of group CH. Microangiography demonstrated no avascular bone segments in group C and in the fibula flaps of group CH. The vascularized ribs of group CH presented with 20 percent avascular bone segments. Biomechanical tests focusing on the durability of the vascularized grafts against bending and torsion forces demonstrated a reduction of the average maximum bending times by 17 percent and 23.9 percent compared to the controls ( P < 0.05). The twisting times were reduced by 13.8 percent (n.s.) and 32.5 percent ( P < 0.05). The data demonstrated a clear worsening in bone healing and stability after simulated adjuvant and neo-adjuvant chemotherapy. Thus, a large animal model was established for the further determination of the effects of chemotherapy on different vascularized bone transfers.
authors.affiliation: Department for Hand-, Replantation- and Microsurgery, Unfallkrankenhaus Berlin, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Eisenschenk
authors.forename: Christiane
authors.initials: C
authors.lastname: Witzel
authors.forename: Martin
authors.initials: M
authors.lastname: Lautenbach
authors.forename: Axel
authors.initials: A
authors.lastname: Ekkernkamp
authors.forename: Ulrich
authors.initials: U
authors.lastname: Weber
authors.forename: Markus V
authors.initials: MV
authors.lastname: Küntscher
mesh_terms: D000792:Angiography; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001696:Biomechanical Phenomena; D016025:Bone Transplantation / Q000473:pathology*; D001842:Bone and Bones / Q000187:drug effects*; D017024:Chemotherapy, Adjuvant; D004285:Dogs; D004317:Doxorubicin / Q000494:pharmacology; D005360:Fibula; D008297:Male; D008727:Methotrexate / Q000494:pharmacology; D008852:Microradiography; D023421:Models, Animal; D020360:Neoadjuvant Therapy; D018583:Pliability; D012272:Ribs; D019415:Torque; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000903:Antibiotics, Antineoplastic; D000964:Antimetabolites, Antineoplastic; D000970:Antineoplastic Agents; D004317:Doxorubicin; D008727:Methotrexate