[The relationship of nature killer cell 2D gene polymorphisms and ulcerative colitis in the Chinese].
OBJECTIVE
Nature killer cell 2D (NKG2D) is an important activated cytokine that has been implicated in the inflammatory reaction and immune response. The function and the location of NKG2D gene show it is an ideal susceptibility gene to ulcerative colitis (UC). We evaluated the NKG2D gene polymorphisms in patients of Zhejiang province to determine whether the gene is associated with susceptibility to UC in Chinese Han population.


METHODS
Blood samples were obtained from 110 patients with UC and 292 healthy controls in Zhejiang location. Genotyping for 2 common NKG2D (10676G/, 908A/) polymorphisms was carried out using polymerase chain sequence with specific primer.


RESULTS
NKG2D was not associated with disease (908A allele frequency patients 19.1% vs controls 16.3%, P > 0.05). None of the patients with UC had 10676G heterozygous or homozygous variants. Similarly none of the health controls.


CONCLUSIONS
The common variants in NKG2D are not associated with UC in the Chinese Han population. Research of larger sample's queue, analysis from different layer and DNA sequence will help to determine the function of NKG2D in the process of UC.
Cao
Qian
Q
Department of Gastroenterology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310016, China.
Shen
Xiao-ling
XL
Zhou
Wen
W
Wang
Jian-guo
JG
D000483:Alleles; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D003093:Colitis, Ulcerative / Q000453:epidemiology / Q000235:genetics*; D003602:Cytotoxicity, Immunologic / Q000235:genetics*; D005787:Gene Frequency; D006801:Humans; D007694:Killer Cells, Natural / Q000378:metabolism*; D011110:Polymorphism, Genetic