Retinal and olfactory bulb precursor cells show distinct responses to FGF-2 and laminin.
We analyzed whether the embryonic (E12.5-E14.5) mouse retina possesses genuine neural stem cells and how they respond to defined growth factors and extracellular matrix molecules. Whereas most combinations produced no or limited cell survival and proliferation in culture, FGF-2 plus heparin and laminin stimulated proliferation and the formation of aggregates composed, after two days, of 95.2% nestin-positive cells. However, cells in these aggregates could only be passaged poorly, lost nestin expression and proliferative capacity, and differentiated into neurons. Under the same conditions, olfactory bulb precursor cells divided efficiently and could be expanded. These data suggest that, in addition to FGF-2 and laminin, embryonic retinal neuroepithelial cells need additional extrinsic and/or intrinsic regulators to maintain cell proliferation and self-renewal.
Otaegi
Gaizka
G
Cellular and Molecular Physiopathology Department, Centro de Investigaciones Biológicas (CIB), Consejo Superior de Investigaciones Científicas (CSIC), C/Ramiro de Maeztu 9, E-28040 Madrid, Spain.
de Pablo
Flora
F
Vicario-Abejón
Carlos
C
de la Rosa
Enrique J
EJ
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006493:Heparin / Q000494:pharmacology; D006657:Histones / Q000032:analysis; D007381:Intermediate Filament Proteins / Q000032:analysis; D007797:Laminin / Q000494:pharmacology*; D051379:Mice; D009419:Nerve Tissue Proteins / Q000032:analysis; D064231:Nestin; D009830:Olfactory Bulb / Q000187:drug effects* / Q000196:embryology; D012160:Retina / Q000166:cytology / Q000187:drug effects* / Q000196:embryology; D013234:Stem Cells / Q000187:drug effects*; D014404:Tubulin / Q000032:analysis
D015415:Biomarkers; D003470:Culture Media; D006657:Histones; D007381:Intermediate Filament Proteins; D007797:Laminin; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D014404:Tubulin; C462941:beta3 tubulin, mouse; D016222:Fibroblast Growth Factor 2; D006493:Heparin