title: In-vitro and in-vivo anti-inflammatory action of the ethanol extract of Trachelospermi caulis.
abstract: In this study, we aimed to investigate the anti-inflammatory activity, antinociceptive activity and the action mechanism of Trachelospermi caulis extract. The anti-inflammatory effects were investigated using arachidonic acid, 12-O-tetradecanoylphorbol 13-acetate or carrageenan-induced oedema assays. Antinociceptive activity, using the acetic acid-induced writhing model, was also tested in mice. The extract exhibited dose-dependent and significant (P<0.05 at 100-400 mg kg-1) anti-inflammatory and antinociceptive activity in the animals. To further understand the mechanism of activity, we investigated whether the extract inhibited the expression of inducible nitric oxide synthase (iNOS), the production of nitric oxide (NO) and the expression of TNF-alpha from murine macrophage RAW 264.7 cells. Similar to the in-vivo activity, the iNOS expression, NO production and TNF-alpha expression were found to be dose dependent and significantly suppressed by the extract through the inhibition of the p38 MAP kinase/NF-kappaB pathway. Taken together, the results presented here suggest that T. caulis extract may be useful for the treatment of various inflammatory diseases.
authors.affiliation: Life Science R&D Center, Sinil Pharmaceutical Co., Ltd., Chungju 380-862, Korea.
authors.forename: Mu Hong
authors.initials: MH
authors.lastname: Lee
authors.forename: Jeong Min
authors.initials: JM
authors.lastname: Lee
authors.forename: Sung Hoon
authors.initials: SH
authors.lastname: Jun
authors.forename: Chul Gyu
authors.initials: CG
authors.lastname: Ha
authors.forename: Seung-Ha
authors.initials: SH
authors.lastname: Lee
authors.forename: Nam Wook
authors.initials: NW
authors.lastname: Kim
authors.forename: Jun Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Na Young
authors.initials: NY
authors.lastname: Ko
authors.forename: Se Hwan
authors.initials: SH
authors.lastname: Mun
authors.forename: Seung Hwa
authors.initials: SH
authors.lastname: Park
authors.forename: Bo Kyung
authors.initials: BK
authors.lastname: Kim
authors.forename: Erk
authors.initials: E
authors.lastname: Her
authors.forename: Young Mi
authors.initials: YM
authors.lastname: Kim
authors.forename: Wahn Soo
authors.initials: WS
authors.lastname: Choi
mesh_terms: D019342:Acetic Acid; D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D016718:Arachidonic Acid; D002351:Carrageenan; D002460:Cell Line; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy*; D000431:Ethanol / Q000737:chemistry; D005663:Furans / Q000032:analysis; D005960:Glucosides / Q000032:analysis; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009569:Nitric Oxide / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy*; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013755:Tetradecanoylphorbol Acetate; D051996:Transcription Factor RelA / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D005663:Furans; D005960:Glucosides; D010936:Plant Extracts; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; D016718:Arachidonic Acid; D009569:Nitric Oxide; D000431:Ethanol; D002351:Carrageenan; D052247:Nitric Oxide Synthase Type II; D048051:p38 Mitogen-Activated Protein Kinases; D013755:Tetradecanoylphorbol Acetate; D019342:Acetic Acid; C077992:arctiin