title: Large-scale mapping of mutations affecting zebrafish development.
abstract: BACKGROUND
  Large-scale mutagenesis screens in the zebrafish employing the mutagen ENU have isolated several hundred mutant loci that represent putative developmental control genes. In order to realize the potential of such screens, systematic genetic mapping of the mutations is necessary. Here we report on a large-scale effort to map the mutations generated in mutagenesis screening at the Max Planck Institute for Developmental Biology by genome scanning with microsatellite markers.
  
  
  RESULTS
  We have selected a set of microsatellite markers and developed methods and scoring criteria suitable for efficient, high-throughput genome scanning. We have used these methods to successfully obtain a rough map position for 319 mutant loci from the Tübingen I mutagenesis screen and subsequent screening of the mutant collection. For 277 of these the corresponding gene is not yet identified. Mapping was successful for 80 % of the tested loci. By comparing 21 mutation and gene positions of cloned mutations we have validated the correctness of our linkage group assignments and estimated the standard error of our map positions to be approximately 6 cM.
  
  
  CONCLUSIONS
  By obtaining rough map positions for over 300 zebrafish loci with developmental phenotypes, we have generated a dataset that will be useful not only for cloning of the affected genes, but also to suggest allelism of mutations with similar phenotypes that will be identified in future screens. Furthermore this work validates the usefulness of our methodology for rapid, systematic and inexpensive microsatellite mapping of zebrafish mutations.
authors.affiliation: Department 3--Genetics, Max-Planck-Institut für Entwicklungsbiologie, Spemannstr, 35/III, 72076 Tübingen, Germany. robert.geisler@tuebingen.mpg.de
authors.forename: Robert
authors.initials: R
authors.lastname: Geisler
authors.forename: Gerd-Jörg
authors.initials: GJ
authors.lastname: Rauch
authors.forename: Silke
authors.initials: S
authors.lastname: Geiger-Rudolph
authors.forename: Andrea
authors.initials: A
authors.lastname: Albrecht
authors.forename: Frauke
authors.initials: F
authors.lastname: van Bebber
authors.forename: Andrea
authors.initials: A
authors.lastname: Berger
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Busch-Nentwich
authors.forename: Ralf
authors.initials: R
authors.lastname: Dahm
authors.forename: Marcus P S
authors.initials: MP
authors.lastname: Dekens
authors.forename: Christopher
authors.initials: C
authors.lastname: Dooley
authors.forename: Alexandra F
authors.initials: AF
authors.lastname: Elli
authors.forename: Ines
authors.initials: I
authors.lastname: Gehring
authors.forename: Horst
authors.initials: H
authors.lastname: Geiger
authors.forename: Maria
authors.initials: M
authors.lastname: Geisler
authors.forename: Stefanie
authors.initials: S
authors.lastname: Glaser
authors.forename: Scott
authors.initials: S
authors.lastname: Holley
authors.forename: Matthias
authors.initials: M
authors.lastname: Huber
authors.forename: Andy
authors.initials: A
authors.lastname: Kerr
authors.forename: Anette
authors.initials: A
authors.lastname: Kirn
authors.forename: Martina
authors.initials: M
authors.lastname: Knirsch
authors.forename: Martina
authors.initials: M
authors.lastname: Konantz
authors.forename: Axel M
authors.initials: AM
authors.lastname: Küchler
authors.forename: Florian
authors.initials: F
authors.lastname: Maderspacher
authors.forename: Stephan C
authors.initials: SC
authors.lastname: Neuhauss
authors.forename: Teresa
authors.initials: T
authors.lastname: Nicolson
authors.forename: Elke A
authors.initials: EA
authors.lastname: Ober
authors.forename: Elke
authors.initials: E
authors.lastname: Praeg
authors.forename: Russell
authors.initials: R
authors.lastname: Ray
authors.forename: Brit
authors.initials: B
authors.lastname: Rentzsch
authors.forename: Jens M
authors.initials: JM
authors.lastname: Rick
authors.forename: Eva
authors.initials: E
authors.lastname: Rief
authors.forename: Heike E
authors.initials: HE
authors.lastname: Schauerte
authors.forename: Carsten P
authors.initials: CP
authors.lastname: Schepp
authors.forename: Ulrike
authors.initials: U
authors.lastname: Schönberger
authors.forename: Helia B
authors.initials: HB
authors.lastname: Schonthaler
authors.forename: Christoph
authors.initials: C
authors.lastname: Seiler
authors.forename: Samuel
authors.initials: S
authors.lastname: Sidi
authors.forename: Christian
authors.initials: C
authors.lastname: Söllner
authors.forename: Anja
authors.initials: A
authors.lastname: Wehner
authors.forename: Christian
authors.initials: C
authors.lastname: Weiler
authors.forename: Christiane
authors.initials: C
authors.lastname: Nüsslein-Volhard
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D005260:Female; D016678:Genome; D008297:Male; D018895:Microsatellite Repeats; D016296:Mutagenesis; D009154:Mutation; D010641:Phenotype; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics*