Effects of (+)-eudesmin from the stem bark of magnolia kobus DC. var. borealis Sarg. on neurite outgrowth in PC12 cells.
(+)-Eudesmin [4,8-bis(3,4-dimethoxyphenyl)-3,7-dioxabicyclo[3.3.0]octane] was isolated from the stem bark of Magnolia kobus DC. var. borealis Sarg. and found to have neuritogenic activity. 50 microM (+)-eudesmin induced neurite outgrowth and enhanced nerve growth factor (NGF)-mediated neurite outgrowth from PC12 cells. At this concentration, (+)-eudesmin also enhanced NGF-induced neurite-bearing activity and this activity was partially blocked by various protein kinase inhibitors. These included PD98059, a mitogen-activated protein kinase (MAPK) kinase inhibitor. GF109203X, a protein kinase C (PKC) inhibitor and H89, a protein kinase A (PKA) inhibitor. These results suggest that (+)-eudesmin can induce neurite outgrowth from PC12 cells by stimulating up-stream MAPK, PKC and PKA pathways.
Yang
Yoo Jung
YJ
College of Pharmacy and Research Center for Bioresource and Health, Chungbuk National University, Cheongju 361-763, Korea.
Park
Jae In
JI
Lee
Hak-Ju
HJ
Seo
Seon-Mi
SM
Lee
Oh-Kyu
OK
Choi
Don-Ha
DH
Paik
Ki-Hyon
KH
Lee
Myung Koo
MK
D000818:Animals; D005419:Flavonoids / Q000494:pharmacology; D005663:Furans / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007211:Indoles / Q000494:pharmacology; D007546:Isoquinolines / Q000494:pharmacology; D017705:Lignans / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D031566:Magnolia / Q000737:chemistry*; D008301:Maleimides / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects*; D016716:PC12 Cells; D024301:Plant Bark / Q000737:chemistry*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D051381:Rats; D013449:Sulfonamides / Q000494:pharmacology
D005419:Flavonoids; D005663:Furans; D007211:Indoles; D007546:Isoquinolines; D017705:Lignans; D008301:Maleimides; D047428:Protein Kinase Inhibitors; D013449:Sulfonamides; C105875:eudesmin; C070515:bisindolylmaleimide I; C063509:N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one