title: [Karyotype analysis in 119 patients with chronic myeloid leukemia at blast crisis].
abstract: To investigate the karyotype of chronic myeloid leukemia in blast crisis (CML-BC), karyotype analysis was performed with R-banding technique in 119 patients with CML-BC. Dual fusion fluorescence in situ hybridization (FISH) was used to detect derivative chromosome 9 deletions in randomly selected 28 cases of them. The results showed that 11 cases (8.9%) were Ph negative; 113 cases (91.1%) were Ph positive; 104 cases (83.9%) had standard Ph translocation; 9 cases (7.2%) showed variant translocation, including 4 cases (3.2%) with simple variant translocation and 5 cases (4.0%) with complex variant translocation. 72.6% of Ph negative CML-BC had extra chromosomal abnormalities, with the most common ones being i (17q) and +14. 72.3% of Ph positive CML-BC had extra cytogenetic changes and +Ph, +8, i (17q) were the most frequent. The average time between diagnosis and blast crisis was 29.0 months (range 2 to 66 months) for Ph negative cases and 34.2 months (range 1 to 127 months) for Ph positive cases. 5 cases (5/28, 17.9%) with der (9) deletions were detected by FISH technique. It is concluded that extra chromosomal abnormalities are common in CML-BC patients and FISH can effectively detect the der (9) deletions.
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province People Hospital, Nanjing 210029, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhu
authors.forename: Jian-Yong
authors.initials: JY
authors.lastname: Li
authors.forename: Jin-Lan
authors.initials: JL
authors.lastname: Pan
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Hai-Rong
authors.initials: HR
authors.lastname: Qiu
authors.forename: Ya-Fang
authors.initials: YF
authors.lastname: Wu
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
authors.forename: Jian-Fu
authors.initials: JF
authors.lastname: Zhang
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Chen
authors.forename: Yong-Quan
authors.initials: YQ
authors.lastname: Xue
mesh_terms: D000328:Adult; D001752:Blast Crisis / Q000235:genetics*; D002872:Chromosome Deletion; D002899:Chromosomes, Human, Pair 9 / Q000235:genetics*; D005260:Female; D006801:Humans; D007621:Karyotyping; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D008297:Male