Evidence for the involvement of carbon-centered radicals in the induction of apoptotic cell death by artemisinin compounds.
Artemisinin and its derivatives are currently recommended as first-line antimalarials in regions where Plasmodium falciparum is resistant to traditional drugs. The cytotoxic activity of these endoperoxides toward rapidly dividing human carcinoma cells and cell lines has been reported, and it is hypothesized that activation of the endoperoxide bridge by an iron(II) species, to form C-centered radicals, is essential for cytotoxicity. The studies described here have utilized artemisinin derivatives, dihydroartemisinin, 10beta-(p-bromophenoxy)dihydroartemisinin, and 10beta-(p-fluorophenoxy)dihydroartemisinin, to determine the chemistry of endoperoxide bridge activation to reactive intermediates responsible for initiating cell death and to elucidate the molecular mechanism of cell death. These studies have demonstrated the selective cytotoxic activity of the endoperoxides toward leukemia cell lines (HL-60 and Jurkat) over quiescent peripheral blood mononuclear cells. Deoxy-10beta-(p-fluorophenoxy)dihydroartemisinin, which lacks the endoperoxide bridge, was 50- and 130-fold less active in HL-60 and Jurkat cells, respectively, confirming the importance of this functional group for cytotoxicity. We have shown that chemical activation is responsible for cytotoxicity by using liquid chromatography-mass spectrometry analysis to monitor endoperoxide activation by measurement of a stable rearrangement product of endoperoxide-derived radicals, which was formed in sensitive HL-60 cells but not in insensitive peripheral blood mononuclear cells. In HL-60 cells the endoperoxides induce caspase-dependent apoptotic cell death characterized by concentration- and time-dependent mitochondrial membrane depolarization, activation of caspases-3 and -7, sub-G(0)/G(1) DNA formation, and attenuation by benzyloxycarbonyl-VAD-fluoromethyl ketone, a caspase inhibitor. Overall, these results indicate that endoperoxide-induced cell death is a consequence of activation of the endoperoxide bridge to radical species, which triggers caspase-dependent apoptosis.
Mercer
Amy E
AE
Department of Pharmacology and Therapeutics, University of Liverpool, Liverpool L69 3GE.
Maggs
James L
JL
Department of Pharmacology and Therapeutics, University of Liverpool, Liverpool L69 3GE.
Sun
Xiao-Ming
XM
Medical Research Council Toxicology Unit, Leicester LE1 9HN, United Kingdom.
Cohen
Gerald M
GM
Medical Research Council Toxicology Unit, Leicester LE1 9HN, United Kingdom.
Chadwick
James
J
Department of Chemistry, the University of Liverpool, Liverpool L69 3GE.
O'Neill
Paul M
PM
Department of Chemistry, the University of Liverpool, Liverpool L69 3GE.
Park
B Kevin
BK
Department of Pharmacology and Therapeutics, University of Liverpool, Liverpool L69 3GE. Electronic address: b.k.park@liv.ac.uk.
D000962:Antimalarials / Q000633:toxicity; D017209:Apoptosis / Q000187:drug effects*; D019306:Artemisia / Q000737:chemistry; D037621:Artemisinins / Q000633:toxicity*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000378:metabolism; D005609:Free Radicals / Q000378:metabolism / Q000633:toxicity; D018922:HL-60 Cells; D006801:Humans; D019169:Jurkat Cells; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D012717:Sesquiterpenes / Q000633:toxicity*; D013778:Tetrazolium Salts / Q000737:chemistry / Q000378:metabolism; D013844:Thiazoles / Q000737:chemistry / Q000378:metabolism
D000962:Antimalarials; D037621:Artemisinins; D004365:Drugs, Chinese Herbal; D005609:Free Radicals; D012717:Sesquiterpenes; D013778:Tetrazolium Salts; D013844:Thiazoles; C031327:artemisinin; D007770:L-Lactate Dehydrogenase