Photogenerated glycan arrays identify immunogenic sugar moieties of Bacillus anthracis exosporium.
Using photogenerated glycan arrays, we characterized a large panel of synthetic carbohydrates for their antigenic reactivities with pathogen-specific antibodies. We discovered that rabbit IgG antibodies elicited by Bacillus anthracis spores specifically recognize a tetrasaccharide chain that decorates the outermost surfaces of the B. anthracis exosporium. Since this sugar moiety is highly specific for the spores of B. anthracis, it appears to be a key biomarker for detection of B. anthracis spores and development of novel vaccines that target anthrax spores.
Wang
Denong
D
Carbohydrate Microarray Laboratory, Stanford University School of Medicine, Stanford, CA, USA. dwang1@stanford.edu
Carroll
Gregory T
GT
Turro
Nicholas J
NJ
Koberstein
Jeffrey T
JT
Kovác
Pavol
P
Saksena
Rina
R
Adamo
Roberto
R
Herzenberg
Leonore A
LA
Herzenberg
Leonard A
LA
Steinman
Lawrence
L
D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology*; D001408:Bacillus anthracis / Q000737:chemistry / Q000276:immunology*; D008027:Light; D046228:Microarray Analysis; D011134:Polysaccharides / Q000737:chemistry / Q000276:immunology*; D011817:Rabbits; D013171:Spores, Bacterial / Q000737:chemistry / Q000276:immunology
D000942:Antigens, Bacterial; D011134:Polysaccharides