title: Cloning and characterization of the ribosomal protein L3 (RPL3) gene family from Triticum aestivum.
abstract: Plant pathogenic fungi of the genus Fusarium can cause severe diseases on small grain cereals and maize. The contamination of harvested grain with Fusarium mycotoxins is a threat to human and animal health. In wheat production of the toxin deoxynivalenol (DON), which inhibits eukaryotic protein biosynthesis, is a virulence factor of Fusarium, and resistance against DON is considered to be part of Fusarium resistance. Previously, single amino acid changes in RPL3 (ribosomal protein L3) conferring DON resistance have been described in yeast. The goal of this work was to characterize the RPL3 gene family from wheat and to investigate the potential role of naturally existing RPL3 alleles in DON resistance by comparing Fusarium-resistant and susceptible cultivars. The gene family consists of three homoeologous alleles of both RPL3A and RPL3B, which are located on chromosomes 4A (RPL3-B2), 4B (RPL3-B1), 4D (RPL3-B3), 5A (RPL3-A3), 5B (RPL3-A2) and 5D (RPL3-A1). Alternative splicing was detected in the TaRPL3-A2 gene. Sequence comparison revealed no amino acid differences between cultivars differing in Fusarium resistance. While using developed SNP markers we nevertheless found that one of the genes, namely, TaRPL3-A3 mapped close to a Fusarium resistance QTL (Qfhs.ifa-5A). The potential role of the RPL3 gene family in DON resistance of wheat is discussed.
authors.affiliation: Institute of Applied Genetics and Cell Biology, Department of Applied Plant Sciences and Plant Biotechnology, BOKU - University of Natural Resources and Applied Life Sciences, Muthgasse 18, Vienna, Austria. doris.lucyshyn@boku.ac.at
authors.forename: Doris
authors.initials: D
authors.lastname: Lucyshyn
authors.forename: Bernhard L
authors.initials: BL
authors.lastname: Busch
authors.forename: Shamsozoha
authors.initials: S
authors.lastname: Abolmaali
authors.forename: Barbara
authors.initials: B
authors.lastname: Steiner
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Chandler
authors.forename: Forough
authors.initials: F
authors.lastname: Sanjarian
authors.forename: Amir
authors.initials: A
authors.lastname: Mousavi
authors.forename: Paul
authors.initials: P
authors.lastname: Nicholson
authors.forename: Hermann
authors.initials: H
authors.lastname: Buerstmayr
authors.forename: Gerhard
authors.initials: G
authors.lastname: Adam
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D003001:Cloning, Molecular; D004351:Drug Resistance / Q000235:genetics; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational; D040641:Quantitative Trait Loci; D000090822:Ribosomal Protein L3; D012269:Ribosomal Proteins / Q000235:genetics* / Q000378:metabolism; D014255:Trichothecenes / Q000633:toxicity; D014908:Triticum / Q000187:drug effects / Q000235:genetics*
chemical_list: D010940:Plant Proteins; C000716547:RPL3 protein, human; D000090822:Ribosomal Protein L3; D012269:Ribosomal Proteins; D014255:Trichothecenes; C007262:deoxynivalenol