Petrotoga halophila sp. nov., a thermophilic, moderately halophilic, fermentative bacterium isolated from an offshore oil well in Congo.
A novel thermophilic, moderately halophilic, rod-shaped bacterium, strain MET-B(T), with a sheath-like outer structure (toga) was isolated from an offshore oil-producing well in Congo, West Africa. Strain MET-B(T) was a Gram-negative bacterium with the ability to reduce elemental sulfur, but not sulfate, thiosulfate or sulfite into sulfide. The optimum growth conditions were 60 degrees C, pH 6.7-7.2 and 4-6 % NaCl. The DNA G+C content was 34.6 mol%. Strain MET-B(T) was phylogenetically related to members of the genus Petrotoga; Petrotoga miotherma, Petrotoga olearia and Petrotoga mexicana were the closest relatives, with type strains exhibiting more than 99 % identity in an analysis of small-subunit rRNA gene sequences. The values for DNA-DNA relatedness between the type strains of these three species and strain MET-B(T) were less than 42 %. As MET-B(T) was found to be genetically and physiologically different from other species of the genus Petrotoga, this strain is proposed as representing a novel species, for which the name Petrotoga halophila sp. nov. is proposed. The type strain is MET-B(T) (=DSM 16923(T)=CCUG 50214(T)).
Miranda-Tello
Elizabeth
E
El Colegio de la Frontera Sur, Unidad Chetumal, Departamento de Biotecnología Ambiental, Ecología Microbiana Aplicada y Contaminación, Av. del Centenario km 5.5, Col. Calderitas, CP 77900, Chetumal, Quintana Roo, Mexico.|IRD, UMR 180, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL case 925, 163 Avenue de Luminy, F-13288 Marseille cedex 09, France.
Fardeau
Marie-Laure
ML
IRD, UMR 180, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL case 925, 163 Avenue de Luminy, F-13288 Marseille cedex 09, France.
Joulian
Catherine
C
BRGM, Environment and Process Division, Biotechnology Unit, BP 36009, F-45060 Orléans, France.|IRD, UMR 180, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL case 925, 163 Avenue de Luminy, F-13288 Marseille cedex 09, France.
Magot
Michel
M
Université de Pau et des Pays de l'Adour, Laboratoire d'Ecologie Moléculaire EA3525, F-64013 Pau, France.
Thomas
Pierre
P
IRD, UMR 180, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL case 925, 163 Avenue de Luminy, F-13288 Marseille cedex 09, France.
Tholozan
Jean-Luc
JL
IRD, UMR 180, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL case 925, 163 Avenue de Luminy, F-13288 Marseille cedex 09, France.
Ollivier
Bernard
B
IRD, UMR 180, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL case 925, 163 Avenue de Luminy, F-13288 Marseille cedex 09, France.
D015373:Bacterial Typing Techniques; D003223:Congo; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D016965:Gram-Negative Anaerobic Straight, Curved, and Helical Rods / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D006358:Hot Temperature; D008969:Molecular Sequence Data; D010578:Petroleum; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010578:Petroleum; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride