Electrophysiologic changes in dorsal root ganglion neurons and behavioral changes in a lumbar radiculopathy model.
METHODS
The DRG neuron was electrophysiologically investigated using a rat model with constriction of the proximal site of the DRG.


OBJECTIVE
To investigate the pathomechanisms of lumbar radiculopathy, we established a rat model with constriction of the proximal site of the DRG. And to characterize the DRG neurons in the rat model of lumbar radiculopathy, the physiologic properties regarding action potential, Na, and K current of the DRG neurons were analyzed through the use of patch clamp recordings.


BACKGROUND
In lumbar root constriction models, properties of secondary afferent neurons in the dorsal horn have been investigated. However, the electrical properties of DRG neuron have not been well investigated.


METHODS
To compare the excitability of DRG neurons between root constriction models and sham, we examined the threshold current, action potential (AP) threshold, resting membrane potential (RMP), afterhyperpolarization (AHP), action potential duration 50 (APD50), action potential amplitude, maximum rise time of AP, and pattern of discharges evoked by depolarizing current. We also examined the peak Na current and steady-state Na and K currents with the voltage clamp technique.


RESULTS
The rats in the root constriction group demonstrated mechanical allodynia and thermal hyperalgesia. In measurement of the action potential, lower threshold current, more depolarized RMP, larger AHP, and prolonged APD50 were measured in the root constriction neurons compared with the sham group. The incidence of sustained burst was significantly higher in root constriction neurons. The Na current in root constriction neurons was markedly larger. There were no significant differences in K current density and voltage dependency.


CONCLUSIONS
The constriction of lumbar root increased excitability and Na current amplitude of DRG neurons. These findings indicate that lumbar radicular pain may be associated with increased excitability of involved DRG neurons.
Kirita
Takashi
T
Department of Orthopaedic Surgery, School of Medicine, Sapporo Medical University, Sapporo, Japan.
Takebayashi
Tsuneo
T
Mizuno
Satoshi
S
Takeuchi
Hirohito
H
Kobayashi
Takeshi
T
Fukao
Mitsuhiro
M
Yamashita
Toshihiko
T
Tohse
Noritsugu
N
D000200:Action Potentials; D000818:Animals; D001522:Behavior, Animal; D003250:Constriction; D004553:Electric Conductivity; D004594:Electrophysiology; D005727:Ganglia, Spinal / Q000503:physiopathology*; D006930:Hyperalgesia / Q000209:etiology; D006941:Hyperesthesia / Q000209:etiology; D008161:Lumbosacral Region; D008297:Male; D008564:Membrane Potentials; D009474:Neurons; D015221:Potassium Channels / Q000378:metabolism; D011843:Radiculopathy / Q000150:complications / Q000209:etiology / Q000503:physiopathology* / Q000523:psychology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time; D015222:Sodium Channels / Q000378:metabolism; D013126:Spinal Nerve Roots
D015221:Potassium Channels; D015222:Sodium Channels