Adipocyte resistin mRNA levels are down-regulated by laparoscopic ovarian electrocautery in both obese and lean women with polycystic ovary syndrome.
BACKGROUND
The aim of this study was to investigate serum and adipocyte mRNA expression of resistin in lean and obese women with polycystic ovary syndrome (PCOS) before and 3 months after laparoscopic ovarian electrocauterization (LOE).


METHODS
Adipose tissue obtained from 12 women with PCOS (six obese and six lean, body mass index > 27 kg m(-1) as threshold point) before and after LOE was analysed. Gene expression of resistin was measured by semi-quantitative RT-PCR. Ten lean, age-matched healthy women served as controls.


RESULTS
Both lean and obese women with PCOS had significantly higher fasting and 2 h insulin and homeostasis model insulin resistance index (HOMA(IR)) values and lower fasting glucose-to-insulin ratios (G(0)/I(0)) than did the controls. The serum levels of glucose and insulin and HOMA(IR) were significantly decreased, and the G(0)/I(0) ratio was significantly increased 3 months after LOE. No difference was found in serum resistin levels between controls and either obese or lean women with PCOS before LOE, nor between PCOS patients before and after LOE. However, resistin mRNA expression levels in both lean and obese women with PCOS before LOE were significantly higher than that in controls and were decreased significantly after LOE back to control levels.


CONCLUSIONS
Local resistin activity may be actively involved in the pathogenesis of PCOS. LOE reduces insulin resistance and down-regulates resistin mRNA expression in lean and obese women with PCOS.
Seow
Kok-Min
KM
Department of Obstetrics and Gynecology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan.
Juan
Chi-Chang
CC
Ho
Low-Tone
LT
Hsu
Yung-Pei
YP
Lin
Yu-Hung
YH
Huang
Lee-Wen
LW
Hwang
Jiann-Loung
JL
D017667:Adipocytes / Q000166:cytology*; D015992:Body Mass Index; D015536:Down-Regulation; D005260:Female; D005786:Gene Expression Regulation; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000378:metabolism; D007333:Insulin Resistance; D010535:Laparoscopy; D009765:Obesity / Q000150:complications; D010053:Ovary / Q000473:pathology*; D011085:Polycystic Ovary Syndrome / Q000175:diagnosis* / Q000628:therapy*; D012333:RNA, Messenger / Q000378:metabolism*; D052243:Resistin / Q000096:biosynthesis*
D007328:Insulin; D012333:RNA, Messenger; D052243:Resistin; D005947:Glucose