title: Indirect amperometric measurement of electroosmotic flow rates and effective mobilities in microchip capillary electrophoresis.
abstract: End-channel indirect amperometry is based on the principle of Kohlrausch regulating function (KRF). A dilute electroactive ionic species is added to the background electrolyte as a continuously eluting electrophore, which is used as probe. The probe concentration variation with the omega value of KRF in the sampling zone was described schematically in this report. Either cathodic or anodic electroosmotic flow (EOF) rates were monitored in microchip. There was no significant difference between the values of EOF rates measured by present method and current-monitoring method. Detection of electroactive and nonelectroactive analytes can also be accomplished by indirect amperometric method. Hence, the effective mobility of analytes can be accurately calculated. And the response mechanism of nonelectroactive analytes K(+), Na(+) and Li(+) in the indirect method was speculated.
authors.affiliation: Key Lab of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210093, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.forename: Jian-Rong
authors.initials: JR
authors.lastname: Zhang
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Zhu
mesh_terms: D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D046209:Electrophoresis, Microchip / Q000379:methods*; D009995:Osmosis