TRB3 modulates C2C12 differentiation by interfering with Akt activation.
TRB3 is a member of TRB protein family characterized by containing a variant kinase domain without enzymatic activity. Interacting with Ser/Thr protein kinases Akt, TRB3 impairs Akt activation induced by growth factors and insulin. In this study we have examined the potential role of TRB3 in muscle differentiation. Our data indicated that the expression of TRB3 is downregulated during C2C12 cells undergoing muscle differentiation and that overexpression of TRB3 inhibits Akt activation during differentiation. Correspondingly, overexpression of TRB3 inhibits, while knockdown TRB3 enhances C2C12 differentiation. Thus, our studies indicated that TRB3 plays a critical role in muscle differentiation.
Kato
Satomi
S
The Molecular Oncology Research Institute, Tufts-New England Medical Center, 750 Washington St., Boston, MA 02111, USA.
Du
Keyong
K
D000818:Animals; D015153:Blotting, Western; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D017570:MyoD Protein / Q000235:genetics / Q000378:metabolism; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism*; D018008:Myogenin / Q000235:genetics / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection
D018797:Cell Cycle Proteins; D017570:MyoD Protein; C057363:MyoD1 myogenic differentiation protein; D018008:Myogenin; D034741:RNA, Small Interfering; D011993:Recombinant Fusion Proteins; C474830:TRB3 protein, mouse; D049452:Green Fluorescent Proteins; D008156:Luciferases; D051057:Proto-Oncogene Proteins c-akt