GC-MS-MS analysis of bacterial fatty acids in heavily creosote-contaminated soil samples.
Phospholipid fatty acid profiles of soil samples enable rapid and reproducible measurement and characterization of the dominant soil microbial communities. When extensive polycyclic aromatic hydrocarbon (PAH) pollution is present in the soil it is very difficult, or even impossible, to distinguish specific fatty acids in GC-MS chromatograms in full-scan mode, because of the PAHs which, because of their lipophilic character, are co-extracted with the lipids. Selected ions in the samples were scanned in MS-MS mode to eliminate the aromatic hydrocarbon signals and obtain clear chromatograms of the fatty acids. By using this technique it was possible to clearly distinguish at least eleven fatty acids in heavily creosote-contaminated soil samples (PAH concentration approximately 15 g kg(-1) dry weight of soil).
Byss
M
M
Laboratory of Environmental Analytical Chemistry, Institute of Systems Biology and Ecology AS CR, Branisovská 31, 370 05 Ceské Budejovice, Czech Republic. Marius.Byss@seznam.cz
Tríska
J
J
Elhottová
D
D
D003407:Creosote / Q000032:analysis*; D005227:Fatty Acids / Q000032:analysis* / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis*; D012987:Soil / Q000032:analysis*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis*; D053719:Tandem Mass Spectrometry
D005227:Fatty Acids; D011084:Polycyclic Aromatic Hydrocarbons; D012987:Soil; D012989:Soil Pollutants; D003407:Creosote