Characterization of a selenate-resistant Arabidopsis mutant. Root growth as a potential target for selenate toxicity.
Screening an Arabidopsis (Arabidopsis thaliana) T-DNA mutant library for selenate resistance enabled us to isolate a selenate-resistant mutant line (sel1-11). Molecular and genetic characterization showed that the mutant contained a lesion in the SULTR1;2 gene that encodes a high affinity root sulfate transporter. We showed that SULTR1;2 is the only gene among 13 mutated genes of the Arabidopsis sulfate transporter family whose mutation conferred selenate resistance to Arabidopsis. The selenate resistance phenotype of the sel1-11 mutant was mirrored by an 8-fold increase of root growth in the presence of selenate as shown by the calculated lethal concentration values. The impairment of SULTR1;2 activity in sel1-11 resulted in a reduced (35)S-sulfate uptake capacity by both roots and calli and a reduced sulfate and selenate content in root, shoot, and calli. Comparing sulfate-to-selenate ratios instead of absolute sulfate and selenate contents in roots and shoots enabled us to gain better insight into the mechanism of selenate toxicity in Arabidopsis. Roots of the sel1-11 mutant line showed a higher sulfate to selenate ratio than that of wild-type roots, while there were no significant differences in sulfate to selenate ratios in shoots of wild-type and mutant lines. These results indicated that the mechanism that confers the selenate resistance phenotype to the sel1-11 line takes place rather in the roots. It might be in part the result of a lower selenate uptake and of a protective effect of sulfate against the toxic effects of selenate on root growth. These results revealed in plants a central and specific role of the transporter SULTR1;2 in selenate sensitivity; they further suggested that root growth and potentially the root tip activity might be a specific target of selenate toxicity in Arabidopsis.
El Kassis
Elie
E
Laboratoire de Biochimie et Physiologie Moléculaire des Plantes, Ecole Nationale Supérieure Agronomique de Montpellier, Institut National de la Recherche Agronomique, Université Montpellier II, Montpellier, France.
Cathala
Nicole
N
Rouached
Hatem
H
Fourcroy
Pierre
P
Berthomieu
Pierre
P
Terry
Norman
N
Davidian
Jean-Claude
JC
D027321:Anion Transport Proteins / Q000235:genetics / Q000502:physiology*; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics / Q000502:physiology*; D001483:Base Sequence; D002874:Chromosome Mapping; D015723:Gene Library; D008969:Molecular Sequence Data; D009154:Mutation; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D064586:Selenic Acid; D018036:Selenium Compounds / Q000494:pharmacology*
D027321:Anion Transport Proteins; D029681:Arabidopsis Proteins; D018036:Selenium Compounds; C455460:Sultr1;2 protein, Arabidopsis; D064586:Selenic Acid