Combining passive sampling and toxicity testing for evaluation of mixtures of polar organic chemicals in sewage treatment plant effluent.
Effluent from sewage treatment plants has been associated with a range of pollutant effects. Depending on the influent composition and treatment processes the effluent may contain a myriad of different chemicals which makes monitoring very complex. In this study we aimed to monitor relatively polar organic pollutant mixtures using a combination of passive sampling techniques and a set of biochemistry based assays covering acute bacterial toxicity (Microtox), phytotoxicity (Max-I-PAM assay) and genotoxicity (umuC assay). The study showed that all of the assays were able to detect effects in the samples and allowed a comparison of the two plants as well as a comparison between the two sampling periods. Distinct improvements in water quality were observed in one of the plants as result of an upgrade to a UV disinfection system, which improved from 24x sample enrichment required to induce a 50% response in the Microtox assay to 84x, from 30x sample enrichment to induce a 50% reduction in photosynthetic yield to 125x, and the genotoxicity observed in the first sampling period was eliminated. Thus we propose that biochemical assay techniques in combination with time integrated passive sampling can substantially contribute to the monitoring of polar organic toxicants in STP effluents.
Muller
Renee
R
National Research Centre for Environmental Toxicology, The University of Queensland, 39 Kessels Rd., Brisbane, QLD 4108, Australia. r.muller@uq.edu.au
Tang
Janet Y M
JY
Thier
Ricarda
R
Mueller
Jochen F
JF
D001419:Bacteria / Q000187:drug effects / Q000235:genetics; D004784:Environmental Monitoring / Q000379:methods; D009152:Mutagenicity Tests / Q000379:methods; D009930:Organic Chemicals / Q000032:analysis / Q000633:toxicity*; D010944:Plants / Q000187:drug effects / Q000235:genetics; D015203:Reproducibility of Results; D012722:Sewage / Q000737:chemistry*; D018675:Toxicity Tests / Q000379:methods*
D009930:Organic Chemicals; D012722:Sewage