Inhibitory effects of acidic xylooligosaccharide on stress-induced gastric inflammation in mice.
The preventive effects of acidic xylooligosaccharide prepared from xylan of corncobs and related sugars on stress-induced gastric inflammation in mice were investigated. Oral administration of acidic xylooligosaccharide and hydrocortisone at doses of 100 and 200 mg/kg body weight significantly reduced the number of bleeding points in the gastric mucosa of mice loaded with cold-restraint stress. Acidic xylooligosaccharide showed concentration-dependent superoxide anion radical-scavenging activity at concentrations of 3.3-4.3 mg/mL and its IC50 was 3.5 mg/mL, although this value is approximately six times that of quercetin. The antioxidant activity of acidic xylooligosaccharide could contribute, in part, to its suppressive activities on stress-induced mouse gastritis. Xylose, xylobiose, xylan, and glucuronic acid showed no significant suppressive activities on mouse gastric inflammation at a dose of 100 mg/kg body weight. These results suggest that an appropriate degree of polymerization of xylan (larger than trimer) is necessary for the activities of acidic xylooligosaccharide.
Yoshino
Kyoji
K
Department of Chemistry and Biochemistry, Numazu College of Technology, 3600, Ooka, Numazu, Shizuoka 410-8501, Japan.
Higashi
Naoki
N
Koga
Kunimasa
K
D000818:Animals; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers; D005756:Gastritis / Q000209:etiology* / Q000517:prevention & control*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009844:Oligosaccharides / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D011108:Polymers; D013312:Stress, Physiological / Q000150:complications*; D013329:Structure-Activity Relationship; D014990:Xylans / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D003313:Zea mays / Q000737:chemistry
D016166:Free Radical Scavengers; D009844:Oligosaccharides; D011108:Polymers; D014990:Xylans