Identification of a novel HIV type 1 CRF01_AE cytotoxic T lymphocyte (CTL) epitope restricted by an HLA-Cw0602 allele and a novel HLA-A0206/peptide restriction.
This report describes specific T cell responses to HIV-1 CRF01_AE Env and A Gag peptides in 20 HIV-1 CRF01_AE-infected Thai individuals using an interferon-gamma (IFN-gamma) enzyme-linked immunospot (ELISpot) assay. Twenty-six potentially novel HLA class I-restricted CD8+ T cell epitopes were identified in 14/20 subjects. Fine mapping analysis using the chromium release cytotoxic T lymphocyte (CTL) assay revealed a novel HLA-Cw0602 restricted epitope of HIV-1 CRF01_AE Env (NAKTIIVHL) and a previously identified HIV-1 A Gag epitope (ATLEEMMTA) with a novel HLA-A0206 restriction.
Kantakamalakul
Wannee
W
Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand. siwkk@mahidol.ac.th
de Souza
Mark
M
Bejrachandra
Sasitorn
S
Ampol
Silawun
S
Cox
Josephine
J
Sutthent
Ruengpung
R
D018414:CD8-Positive T-Lymphocytes / Q000821:virology*; D018984:Epitopes, T-Lymphocyte / Q000276:immunology / Q000302:isolation & purification*; D015686:Gene Products, env / Q000737:chemistry / Q000276:immunology; D015683:Gene Products, gag / Q000737:chemistry / Q000276:immunology; D015658:HIV Infections / Q000097:blood / Q000276:immunology*; D006679:HIV Seropositivity; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D015234:HLA-A Antigens / Q000235:genetics; D015236:HLA-C Antigens / Q000235:genetics; D015395:Histocompatibility Antigens Class I / Q000235:genetics; D006801:Humans; D007371:Interferon-gamma / Q000302:isolation & purification; D020539:Sequence Analysis, Protein; D013785:Thailand
D018984:Epitopes, T-Lymphocyte; D015686:Gene Products, env; D015683:Gene Products, gag; D015234:HLA-A Antigens; D015236:HLA-C Antigens; D015395:Histocompatibility Antigens Class I; D007371:Interferon-gamma