title: [An efficient mutational method for photosynthetic bacteria].
abstract: The pigment and auxotrophic mutants of Rhodobacter sphaeroides Y6 were obtained by treatment with ethyl methanesulfonate (EMS) followed by lithium chloride (LiCI). Treatment with 0.081 M EPS and subsequent treatment with 0.071 M LiCI resulted in 12% higher frequency of pigment mutations than application of 0.081 M EMS alone; the frequency of auxotrophic mutations increased 2.5-fold when treatment with lithium chloride was applied. A blue shift 10 nm was recorded in the absorption spectrum of carotenoids form YM5-3 green mutant; considerable accumulation of neurosporine was revealed by HPLC and mass spectrometry. The method is efficient for isolating mutants of photosynthetic bacteria.
authors.forename: J Q
authors.initials: JQ
authors.lastname: Lin
authors.forename: M
authors.initials: M
authors.lastname: Xiao
authors.forename: M T
authors.initials: MT
authors.lastname: Long
authors.forename: B
authors.initials: B
authors.lastname: Han
authors.forename: W
authors.initials: W
authors.lastname: Quian
authors.forename: J
authors.initials: J
authors.lastname: Du
mesh_terms: D005020:Ethyl Methanesulfonate / Q000494:pharmacology*; D018021:Lithium Chloride / Q000494:pharmacology*; D009154:Mutation; D010788:Photosynthesis; D010860:Pigments, Biological / Q000235:genetics; D012242:Rhodobacter sphaeroides / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism
chemical_list: D010860:Pigments, Biological; D005020:Ethyl Methanesulfonate; D018021:Lithium Chloride