Molecular typing by random amplification of polymorphic DNA (RAPD) and detection of virulence genes of Salmonella enterica subspecies enterica serovar Gallinarum biovar gallinarum.
Salmonella enterica subspecies enterica serovar Gallinarum biovar Gallinarum is the causative agent of fowl typhoid in chickens, outbreaks of which have devastated poultry populations in Korea since 1992. In order to identify genetic differences among S. Gallinarum isolates, bacteria were examined using the random amplified polymorphic DNA (RAPD) method. Of 13 arbitrary primers screened initially, the primer designated as universal rice primer-6 (URP-6) was selected for subsequent typing assays because it produced a distinctive and reproducible DNA fingerprint for a S. Gallinarum reference strain. URP-6-based RAPD analysis assigned 30 S. Gallinarum isolates into 6 types, with 26 isolates (86.6%) belonging to 2 major RAPD types. The distribution of virulence genes in S. Gallinarum isolates was examined by Southern hybridization. All tested isolates had the invasion gene, invA, the virulence plasmid gene, spvB, and the S. Enteritidis fimbrial gene, sefC. The distribution of virulence genes among S. Gallinarum isolates did not correlate with any specific RAPD type.
Jin
Ji-Dong
JD
School of Veterinary Medicine, Kangwon National University, Chuncheon, Kangwon-Do, Korea.
Lee
Dong-Seok
DS
Shin
Eun-Kyung
EK
Kim
Sun-Joong
SJ
Jung
Rose
R
Hahn
Tae-Wook
TW
D015373:Bacterial Typing Techniques / Q000379:methods*; D005798:Genes, Bacterial / Q000235:genetics*; D014644:Genetic Variation; D005838:Genotype; D019105:Random Amplified Polymorphic DNA Technique / Q000662:veterinary*; D019779:Salmonella enterica / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity*; D014774:Virulence / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics*
D037521:Virulence Factors