title: Rare carotenoids, (3R)-saproxanthin and (3R,2'S)-myxol, isolated from novel marine bacteria (Flavobacteriaceae) and their antioxidative activities.
abstract: We isolated three orange or yellow pigment-producing marine bacteria, strains 04OKA-13-27 (MBIC08261), 04OKA-17-12 (MBIC08260), and YM6-073 (MBIC06409), off the coast of Okinawa Prefecture in Japan. These strains were classified as novel species of the family Flavobacteriaceae based on their 16S rRNA gene sequence. They were cultured, and the major carotenoids produced were purified by chromatographic methods. Their structures were determined by spectral data to be (3R)-saproxanthin (strain 04OKA-13-27), (3R,2'S)-myxol (strain YM6-073), and (3R,3'R)-zeaxanthin (strains YM6-073 and 04OKA-17-12). Saproxanthin and myxol, which are monocyclic carotenoids rarely found in nature, demonstrated significant antioxidative activities against lipid peroxidation in the rat brain homogenate model and a neuro-protective effect from L-glutamate toxicity.
authors.affiliation: Department of Food and Nutrition, Japan Women's University, 2-8-1 Mejirodai, Tokyo 112-8681, Japan. Kshindo@fc.jwu.ac.jp
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Shindo
authors.forename: Kana
authors.initials: K
authors.lastname: Kikuta
authors.forename: Atsuko
authors.initials: A
authors.lastname: Suzuki
authors.forename: Atsuko
authors.initials: A
authors.lastname: Katsuta
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kasai
authors.forename: Mina
authors.initials: M
authors.lastname: Yasumoto-Hirose
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Matsuo
authors.forename: Norihiko
authors.initials: N
authors.lastname: Misawa
authors.forename: Shinichi
authors.initials: S
authors.lastname: Takaichi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002338:Carotenoids / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D041965:Flavobacteriaceae / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D018698:Glutamic Acid / Q000633:toxicity; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D051381:Rats; D011916:Rats, Inbred F344; D017208:Rats, Wistar; D017422:Sequence Analysis, DNA; D024341:Xanthophylls / Q000737:chemistry / Q000494:pharmacology*; D065146:Zeaxanthins
chemical_list: D000975:Antioxidants; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D024341:Xanthophylls; D065146:Zeaxanthins; C488895:myxol; D002338:Carotenoids; D018698:Glutamic Acid