Effect of heat and high-pressure treatments on microbiological quality and immunoglobulin G stability of caprine colostrum.
Caprine colostrums (6 batches) were subjected to heat (56 degrees C for 60 min and 63 degrees C for 30 min) and high-pressure (400 and 500 MPa for 10 min at 20 degrees C) treatments at laboratory scale, and analyses of the main microbial groups and the extent of IgG denaturation (determined by immunodiffusion) were performed. Overall mean microbial values in raw colostrums were: total count, 5.55 log cfu/mL; Enterobacteriaceae, 2.64 log cfu/mL; lactococci, 5.41 log cfu/mL; lactobacilli, 2.34 log cfu/mL; and enterococci, 4.06 log cfu/mL. Neither Salmonella spp. nor Listeria monocytogenes were detected, whereas coagulase-positive staphylococci were found in various colostrum samples with an overall mean of 1.02 log cfu/mL. Heat and high-pressure treatments significantly reduced total count (1.47 log), lactococci (1.45 log), enterococci (2.47 log), and Enterobacteriaceae, whereas lactobacilli and coagulase-positive staphylococci counts were reduced to undetectable levels, but differences between technological treatments were not statistically significant. High-pressure treatments were as efficient in reducing the bacterial population as were heat pasteurization treatments: 95.50 and 96.93% for pressure treatments of 400 and 500 MPa, and 91.61 and 97.59% for heat treatments of 56 degrees C for 60 min and 63 degrees C for 30 min, respectively. All treatments assayed produced a reduction in colostrum IgG concentration (27.53, 23.58, 23.33, 22.09, and 17.06 mg/mL for raw, heat-treated at 56 degrees C for 60 min or 63 degrees C for 30 min, and pressure-treated at 400 and 500 MPa, respectively), but differences were only observed between raw colostrums and those pressure-treated at 500 MPa. This laboratory-scale study indicated that 20- to 30-mL volumes of goat colostrum could be heated and pressure-treated (400 MPa) to produce hygienic colostrum without affecting IgG concentration.
Trujillo
A J
AJ
Centre Especial de Recerca Planta de Tecnologia dels Aliments (CERPTA), CeRTA, XiT, Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain. Toni.Trujillo@uab.es
Castro
N
N
Quevedo
J M
JM
Argüello
A
A
Capote
J
J
Guamis
B
B
D000818:Animals; D003126:Colostrum / Q000276:immunology* / Q000382:microbiology*; D004355:Drug Stability; D004755:Enterobacteriaceae / Q000302:isolation & purification; D005260:Female; D005511:Food Handling / Q000379:methods; D006041:Goats; D006358:Hot Temperature; D007074:Immunoglobulin G / Q000032:analysis* / Q000737:chemistry; D008089:Listeria monocytogenes / Q000302:isolation & purification; D011312:Pressure; D011489:Protein Denaturation; D012475:Salmonella / Q000302:isolation & purification
D007074:Immunoglobulin G