title: Heterologous expression of the mutated melon ethylene receptor gene Cm-ERS1/H70A produces stable sterility in transgenic lettuce (Lactuca sativa).
abstract: The mutated melon ethylene receptor gene Cm-ERS1/H70A was introduced into tobacco and induced stable sterility in transgenic lines. This gene contains a missense mutation that converts the His(70) residue to Ala in the melon ethylene receptor gene Cm-ERS1. To test the applicability of this inducible sterility system to other plants, lettuce (Lactuca sativa) was transformed with the gene using Agrobacterium, and putative transformants containing Cm-ERS1/H70A were obtained. Thirteen randomly selected putative transformants were grown in a growth room under constant conditions, and seven of the lines showed sterility or significantly reduced fertility. DNA gel blot analysis confirmed the integration of the Cm-ERS1/H70A gene into the genomes of the putative transformants, and RT-PCR and protein gel blot analysis confirmed the expression of Cm-ERS1/H70A mRNA and protein in all of the transformants. Five transformants showing sterility or reduced fertility when grown in a growth room under constant conditions were randomly selected to be grown in an open-air greenhouse under various environmental conditions. All five showed stable sterility under the various conditions. These results suggest that Cm-ERS1/H70A can induce sterility in heterologous transgenic plants.
authors.affiliation: Gene Research Center, Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
authors.forename: Keita
authors.initials: K
authors.lastname: Takada
authors.forename: Shin
authors.initials: S
authors.lastname: Watanabe
authors.forename: Tsunenori
authors.initials: T
authors.lastname: Sano
authors.forename: Biao
authors.initials: B
authors.lastname: Ma
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kamada
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ezura
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics; D001483:Base Sequence; D019662:Cucurbitaceae / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D015870:Gene Expression; D017343:Genes, Plant; D005822:Genetic Vectors; D018545:Lactuca / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D009154:Mutation; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D011956:Receptors, Cell Surface / Q000235:genetics*; D012098:Reproduction / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D010940:Plant Proteins; D011956:Receptors, Cell Surface; C083750:ethylene receptors, plant