title: Evaluation of genetic markers and molecular typing methods for prediction of sources of Campylobacter jejuni and C. coli infections.
abstract: Campylobacter jejuni and C. coli isolates from poultry, cattle, and humans were studied using pulsed-field gel electrophoresis (PFGE) and PCR of candidate livestock-associated marker genes. Human isolates showed 5.7 and 61% overlap with cattle and poultry isolates, respectively, by use of PFGE. No unambiguous association was found between marker genes (the Cj1321 and Cj1324 genes) and livestock-associated isolates.
authors.affiliation: Department of Food and Environmental Hygiene, Faculty of Veterinary Medicine, P.O. Box 66, FI-00014 University of Helsinki, and HUSLAB, Helsinki University Central Hospital Laboratory, Finland. rauni.karenlampi@helsinki.fi
authors.forename: Rauni
authors.initials: R
authors.lastname: Kärenlampi
authors.forename: Hilpi
authors.initials: H
authors.lastname: Rautelin
authors.forename: Marja-Liisa
authors.initials: ML
authors.lastname: Hänninen
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D002169:Campylobacter Infections / Q000382:microbiology* / Q000662:veterinary; D017000:Campylobacter coli / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016123:Campylobacter jejuni / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D002645:Chickens / Q000382:microbiology; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D005243:Feces / Q000382:microbiology; D005506:Food Contamination; D005516:Food Microbiology; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D008460:Meat / Q000382:microbiology; D011201:Poultry Diseases / Q000382:microbiology
chemical_list: D005819:Genetic Markers; D015252:Deoxyribonucleases, Type II Site-Specific; C029312:GGTACC-specific type II deoxyribonucleases