title: Microbiological surveillance of hospital ventilation systems in departments at high risk of nosocomial infections.
abstract: The air in hospital wards with patients at high risk (Surgeries, Intensive Care Units and Bone Marrow Transplant Centers) has been surveyed less than the one in Operating Rooms. Therefore in this study we considered useful to verify the microbic contamination of the air of those wards evaluating the consistency of ventilation systems in relation also to the presence and location of HEPA absolute filters. Seven departments of Genoese San Martino Hospital at high risk of infection were taken into account. In there, environmental investigations have been performed by air samplings and by analyzing bacterial and fungal growth on plates after an incubation period. Almost 60% of all samples taken in wards yielded a positive result and the average values of bacterial and aspergillar charges measured at air flow emission openings decisively exceed the ones considered standard in operating rooms. Still, the average values of airborne bacterial charges were significantly higher in those wards equipped with central filters (p < 0.001), while as far as the aspergillar charge is concerned, no statistically relevant differences were noticed. In wards with ventilation system, the bacterial charge value raises from the emission grids to the middle of the room and to the aspiration grids, while the ward not equipped with a ventilation system presents in the middle of the room an average bacterial charge 2 to 10 times higher than the one in other wards. The average values regarding bacterial and aspergillar charges resulted quite high in all the departments surveyed. Nevertheless, if we take into account ventilation systems equipped with absolute filters HEPA located centrally or peripherally, it can be outlined that the air quality from the point of view of both microbic and aspergillar contamination turns out to be decisively better in systems with peripheral filters. Moreover, a compared analysis of the three Hematology wards allows us to infer that the presence of artificial ventilation systems can lower the bacterial and fungal compared with a ward with natural ventilation.
authors.affiliation: Department of Health Sciences, University of Genoa, Italy. paolo.crimi@hsan-martino.liguria.it
authors.forename: P
authors.initials: P
authors.lastname: Crimi
authors.forename: F
authors.initials: F
authors.lastname: Argellati
authors.forename: G
authors.initials: G
authors.lastname: Macrina
authors.forename: C
authors.initials: C
authors.lastname: Tinteri
authors.forename: L
authors.initials: L
authors.lastname: Copello
authors.forename: D
authors.initials: D
authors.lastname: Rebora
authors.forename: L
authors.initials: L
authors.lastname: Romania
authors.forename: R
authors.initials: R
authors.lastname: Rizzetto
mesh_terms: D000391:Air Microbiology; D016902:Air Pollution, Indoor / Q000032:analysis*; D001230:Aspergillus / Q000302:isolation & purification; D016026:Bone Marrow Transplantation; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology*; D004784:Environmental Monitoring / Q000379:methods*; D062665:Epidemiological Monitoring; D005374:Filtration; D006405:Hematology; D006749:Hospital Design and Construction; D006757:Hospital Units / Q000592:standards*; D006801:Humans; D016867:Immunocompromised Host; D007362:Intensive Care Units; D007558:Italy; D008284:Maintenance and Engineering, Hospital; D011997:Recovery Room; D012307:Risk Factors; D014691:Ventilation / Q000295:instrumentation / Q000592:standards*