Development of an indirect ELISA for the detection of antibodies against Peste-des-petits-ruminants virus in small ruminants.
Peste des petits ruminants (PPR) is an acute, febrile, highly contagious and economically important viral disease of small ruminants. A polyclonal antibody based indirect ELISA was developed for detection of antibodies to PPR virus in the serum samples of goats and sheep using purified PPR viral antigen propagated in Vero cell culture. A threshold (cut-off) value was set as twice the mean of the negative population based on the distribution of known negative serum samples in respect of PPR virus antibodies in the test. A total of 1544 serum samples from goats and sheep were screened by indirect ELISA and competitive ELISA. The indirect ELISA compared very well with competitive ELISA, with a high degree of specificity (95.09%) and sensitivity (90.81%). When compared with virus neutralization test, the present assay had 100% specificity and 80% sensitivity. With serum samples, the assay could clearly differentiate animals from the infected population from uninfected ones. These results suggest that the indirect ELISA may be a good alternative tool to competitive ELISA for seroepidemiological surveys.
Balamurugan
V
V
Division of Virology, Indian Veterinary Research Institute, Mukteswar, Uttaranchal, India. balavirol@rediffmail.com
Singh
R P
RP
Saravanan
P
P
Sen
A
A
Sarkar
J
J
Sahay
B
B
Rasool
T J
TJ
Singh
R K
RK
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D015511:Goat Diseases / Q000097:blood / Q000175:diagnosis / Q000821:virology*; D006041:Goats; D009500:Neutralization Tests / Q000662:veterinary; D029021:Peste-des-Petits-Ruminants / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D018107:Peste-des-petits-ruminants virus / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D012756:Sheep; D012757:Sheep Diseases / Q000097:blood / Q000175:diagnosis / Q000821:virology*
D000914:Antibodies, Viral