title: Simultaneous determination of lamotrigine, zonisamide, and carbamazepine in human plasma by high-performance liquid chromatography.
abstract: A high-performance liquid chromatography assay with ultraviolet detection was developed for the simultaneous determination of the anti-epileptic drugs lamotrigine, carbamazepine and zonisamide in human plasma and serum. Lamotrigine, carbamazepine, zonisamide and the internal standard chloramphenicol were extracted from serum or plasma using liquid-liquid extraction under alkaline conditions into an organic solvent. The method was linear in the range 1-30 microg/mL for lamotrigine, 2-20 microg/mL for carbamazepine, and 1-40 microg/mL for zonisamide. Within- and between-run precision studies demonstrated coefficient of variation <10% at all tested concentrations. Other anti-epileptic medications tested did not interfere with the assay. The method is appropriate for determining lamotrigine, carbamazepine and zonisamide serum or plasma concentrations for therapeutic monitoring.
authors.affiliation: Toxicology and Therapeutic Drug Monitoring Laboratory, Division of Clinical Chemistry, Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA, USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Greiner-Sosanko
authors.forename: Darla R
authors.initials: DR
authors.lastname: Lower
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Virji
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Krasowski
mesh_terms: D000328:Adult; D000927:Anticonvulsants / Q000097:blood*; D002220:Carbamazepine / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007555:Isoxazoles / Q000097:blood*; D000077213:Lamotrigine; D010949:Plasma / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D014227:Triazines / Q000097:blood*; D000078305:Zonisamide
chemical_list: D000927:Anticonvulsants; D007555:Isoxazoles; D014227:Triazines; D002220:Carbamazepine; D000078305:Zonisamide; D000077213:Lamotrigine