title: Differential radioactive quantification of protein abundance ratios between benign and malignant prostate tissues: cancer association of annexin A3.
abstract: A differential quantitative protein expression study, comparing matched prostate cancerous and benign tissues from 31 patients, revealed proteins newly associated with prostate cancer. Average effects for 17 proteins whose abundance was significantly different (p<0.01) across patients ranged from 1.5- to 6.1-fold, and included a number of known cancer markers. The most differentially abundant proteins between cancer and benign samples were isopeptidase T, serum amyloid P (SAP), annexin A3 (ANXA3) and mitochondrial enoyl coenzyme-A hydratase. SAP is restricted to stroma in healthy tissue, and the lower abundance in tumours may be explained by the reduced stromal content. ANXA3 is present in healthy epithelial cells, exhibits strong staining in precancerous prostatic intraepithelial neoplasia, and is relatively less abundant in individual tumour cells of increasing Gleason pattern (GP), despite exhibiting higher overall tissue abundance in tumours. ANXA3 staining was predominantly cytoplasmic, yet nuclear localization was also observed. Strongly staining single cells, possibly phagocytes, were interspersed in highly dedifferentiated GP5 tumour areas among tumour cells without measurable ANXA3. Local recurrent androgen ablation therapy-resistant tumours exhibit heterogenous low levels of ANXA3 staining. Results are discussed focussing on the potential implications for tumour tissues.
authors.affiliation: ProteoSys AG, Mainz, Germany.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Wozny
authors.forename: Klaus
authors.initials: K
authors.lastname: Schroer
authors.forename: Gerhard P
authors.initials: GP
authors.lastname: Schwall
authors.forename: Slobodan
authors.initials: S
authors.lastname: Poznanović
authors.forename: Werner
authors.initials: W
authors.lastname: Stegmann
authors.forename: Klaus
authors.initials: K
authors.lastname: Dietz
authors.forename: Hermann
authors.initials: H
authors.lastname: Rogatsch
authors.forename: Georg
authors.initials: G
authors.lastname: Schaefer
authors.forename: Heidi
authors.initials: H
authors.lastname: Huebl
authors.forename: Helmut
authors.initials: H
authors.lastname: Klocker
authors.forename: André
authors.initials: A
authors.lastname: Schrattenholz
authors.forename: Michael A
authors.initials: MA
authors.lastname: Cahill
mesh_terms: D000328:Adult; D000368:Aged; D017318:Annexin A3 / Q000378:metabolism*; D014408:Biomarkers, Tumor; D003937:Diagnosis, Differential; D006801:Humans; D008297:Male; D008875:Middle Aged; D011470:Prostatic Hyperplasia / Q000175:diagnosis / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D011868:Radioisotopes
chemical_list: D014408:Biomarkers, Tumor; D011868:Radioisotopes; D017318:Annexin A3