Adipogenesis in mouse 3T3L1 cells: the effects of Rtl1 over-expression.
Rtl1 and Dlk1 are two genes in an imprinted gene cluster on mouse chromosome 12. Rtl1 is a paternally expressed gene encoding a retrotransposon-like protein, but as yet, there is no evidence that a protein is produced by this gene although a transcript is produced. Dlk1 is also paternally expressed and it encodes a plasma membrane-bound protein known to act as a negative regulator of adipogenesis. Genes at other imprinted loci often show coordinate expression and may interact at a functional level. We tested the hypothesis that Dlk1 and Rtl1 are functionally related. Rtl1 and Dlk1 mRNA expression patterns show similar time courses of down-regulation during induced adipogenesis of 3T3L1 cells. Despite the coordinate down-regulation of Dlk1 and Rtl1 mRNA levels, Rtl1 over-expression did not affect the time course of adipogenesis or Dlk1 expression. It is concluded that these two genes are neither directly nor indirectly functionally related.
Leeton
L A
LA
CSIRO Livestock Industries, Queensland Bioscience Precinct, 306 Carmody Road, St Lucia, 4067 QLD, Australia. lisa.leeton@csiro.au
Tellam
R L
RL
D041721:3T3-L1 Cells; D050156:Adipogenesis / Q000235:genetics*; D000818:Animals; D002135:Calcium-Binding Proteins; D004251:DNA Transposable Elements / Q000235:genetics*; D015536:Down-Regulation; D015870:Gene Expression; D005786:Gene Expression Regulation; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics*; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism
D002135:Calcium-Binding Proteins; D004251:DNA Transposable Elements; C512925:Dlk1 protein, mouse; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger