[Molecular characteristic marker method of 27 breeds of common Umbelliferae Chinese herb medicinal plants by sequencing rDNA].
OBJECTIVE
To probe a molecular marker method of accrediting fingerprinting of 27 kinds of common Umbelliferae Chinese herb medicinal plants by sequencing rDNA.


METHODS
The rDNA sequences of the 27 breeds of common Umbelliferae Chinese herb medicinal plants were amplified, and were digested by restriction endonuclease, and were seperated via polypropylene electrophoresis, at last 6 breeds of them were sequenced.


RESULTS
The rDNA sequence fragment we gained concluded ITS1, ITS2, 5.8S complete sequence and 18S, 26S part sequence. On the electrophoresis map of PCR products digested by restriction endonuclease MSP I, 27 breeds appeared 16 kinds of characteristic map, 11 of them differ from others; and PCR products digested by restriction endonuclease HaeIII, there appeared 5 kinds of characteristic map among 27 breeds, 3 of them differ from others. The sequenced result of 6 breeds showed genes whose length extented from 652bp to 656bp were acquired. These sequences of 3 breeds which showed the same electrophoresis map after digested by restriction endonuclease HaeIII exhibited great similarity according to similar phylogenetic tree constructed on rDNA sequence.


CONCLUSIONS
The rDNA sequence character is effective molecular marker for classifying the different Umbellerae Chinese herb medicinal plants. And the method of sequencing rDNA surpassed that of restriction fragment long polymorphism (RFLP).
Zhao
Guo-Ping
GP
Medical College, Jinan University, Guangzhou 510632, China.
Qian
San-Qi
SQ
Minoru
Niizeki
N
Liuni
Ishigagawa
I
D029971:Angelica sinensis / Q000145:classification / Q000235:genetics; D019661:Apiaceae / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D004275:DNA, Ribosomal / Q000235:genetics*; D005819:Genetic Markers; D008969:Molecular Sequence Data; D010946:Plants, Medicinal / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D018749:RNA, Plant / Q000235:genetics*; D017422:Sequence Analysis, DNA
D004275:DNA, Ribosomal; D005819:Genetic Markers; D018749:RNA, Plant