Monitoring of hepatitis C virus quasispecies in chronic infection by matrix-assisted laser desorption ionization-time of flight mass spectrometry mutation detection.
Using both a mass spectrometry-based method and the classical method of cloning and sequencing, we demonstrated weekly changes in the hypervariable region 1 quasispecies of a chimpanzee infected with an infectious clone, coinciding with neutralizing antibody emergence. We also used the mass spectrometry method in the clinical follow-up of a chronically infected patient over a 5-year period.
Yea
Carmen
C
Metabolism Research Program, The Hospital for Sick Children, Toronto, Canada.
Bukh
Jens
J
Ayers
Melissa
M
Roberts
Eve
E
Krajden
Mel
M
Tellier
Raymond
R
D000595:Amino Acid Sequence; D000818:Animals; D018420:Ape Diseases / Q000821:virology; D016174:Hepacivirus / Q000145:classification* / Q000235:genetics*; D019698:Hepatitis C, Chronic / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D002679:Pan troglodytes; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics*
D014759:Viral Envelope Proteins; C091015:glycoprotein E2, Hepatitis C virus