Only high concentrations of ethanol affect GABAA receptors of rat cerebellum granule cells in culture.
In the experiments described in the present report, we evaluated the effects of ethanol on the activity of GABAA receptors of cerebellar granule cells in culture. Only very high ethanol concentrations (100-300 mM) showed a clear and significant stimulatory effect on the activity of such receptors. This result was unexpected. In fact, previous reports from other groups would have suggested high ethanol sensitivity of at least one population of GABAA receptors expressed by granule cells.
Casagrande
S
S
Dipartimento di Fisica, Università di Genova, Italy.
Cupello
A
A
Pellistri
F
F
Robello
M
M
D020268:Alcohol-Induced Disorders, Nervous System / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D002492:Central Nervous System Depressants / Q000494:pharmacology; D002525:Cerebellar Cortex / Q000187:drug effects* / Q000378:metabolism; D018118:Chloride Channels / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009433:Neural Inhibition / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000187:drug effects* / Q000378:metabolism; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D002492:Central Nervous System Depressants; D018118:Chloride Channels; D011963:Receptors, GABA-A; D000431:Ethanol; D005680:gamma-Aminobutyric Acid