title: A validated liquid chromatographic method for estimation of solasodine in various Solanum species and market formulations.
abstract: This paper describes an improved liquid chromatographic (LC) method involving a prechromatographic derivatization step for the estimation of solasodine from berries of various Solanum species, market samples of Solanum xanthocarpum herb, extract, and its market formulations. Solasodine has heterocyclic nitrogen but has no conjugated double bonds in its structure. However, in all reported LC methods, detection was made in the ultraviolet range of 200-213 nm. In the present study, a prechromatographic derivatization of solasodine was done by forming an ion-pair complex of the heterocyclic nitrogen using the acidic dye methyl orange and acetate buffer of pH 4.7. Detection could be made in the visible range at 530 nm in this method. The method was validated and successfully applied to determine solasodine content in various plant samples and polyherbal formulations. The relative standard deviation was found to be 0.025% for system precision, and 0.8% for the linearity of the method, and the correlation coefficient was 0.999. Plant samples and market formulations were analyzed and found to contain solasodine in the range of 0.113-0.227% (w/w) on a fresh weight basis in berries; 0.3-1.278% (w/w) and 0.412% (w/w) on a dry weight basis in S. xanthocarpum herb powder and extract, respectively; and 0.245-0.525% (w/w) on dry weight basis in formulations containing S. xanthocarpum herb powder. No matrix interference was encountered. The method was found to be accurate, with a mean recovery of 100.5 +/- 0.83%. The method has good reproducibility and was found to be suitable for estimation of solasodine.
authors.affiliation: K.B. Institute of Pharmaceutical Education and Research, Sector-23, GH-6, Gandhinagar PIN Code 382023, Gujarat, India.
authors.forename: Priti D
authors.initials: PD
authors.lastname: Trivedi
authors.forename: Brijesh C
authors.initials: BC
authors.lastname: Patel
authors.forename: Shivprakash
authors.initials: S
authors.lastname: Rathnam
authors.forename: Kilambi
authors.initials: K
authors.lastname: Pundarikakshudu
mesh_terms: D002138:Calibration; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D010936:Plant Extracts / Q000032:analysis; D012015:Reference Standards; D015203:Reproducibility of Results; D012991:Solanaceous Alkaloids / Q000032:analysis*; D032322:Solanum / Q000737:chemistry*; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
chemical_list: D010936:Plant Extracts; D012991:Solanaceous Alkaloids; D012996:Solutions; C012037:solasodine