title: In vivo and in vitro effects of hyperglycemia on Na+ -K+, Ca+2, Mg+2-dependent ATPases activity in brain synaptosomes of aging rats.
abstract: Cerebral metabolism of glucose, one of the determinants of tissue ATP level, is crucial for the CNS function. The activity of P-type pumps: Na(+), K(+)-ATPase, Ca(+2)-ATPase and Mg(+2)-ATPase were examined in rat brain synaptosomes to determine if changes in the enzyme activity related to aging are potentially associated with alterations in glucose homeostasis. Male Wistar rats (newborn, 3- and 18-month-old) were sacrificed by decapitation and synaptic plasma membranes were isolated from brains. In vivo study demonstrated that 18-month-old rats were characterized by hyperglycemia, hyperinsulinemia and increased total antyoxidative status (TAS) level. These conditions had a different impact on activities of the ATPases tested in vivo: only the activity of Ca(+2)-ATPase decreased whereas that of Mg(+2)-ATPase increased significantly. In vitro experiments, prior incubation of isolated synaptosomes with glucose of concentrations corresponding to normoglycemia in vivo (4.5 - 6.5 mM), stimulated Ca(+2)-ATPase activity, whereas higher glucose concentrations (10.0 - 12.5 mM) inhibited significantly the enzyme activity. The most sensitive to hyperglycemia appeared Na(+), K(+)-ATPase in old rats synaptosomes with the progressive decline starting at 6.5 mM glucose. The activity of Mg(+2)-ATPase was not inhibited in vitro even at high glucose concentrations that may explain the increased in vivo, activity of this enzyme in old, hyperglycemic rats.
authors.affiliation: Department of Physiology, University of Medical Sciences, Poznań, Poland. ttorlin@amp.edu.pl
authors.forename: K
authors.initials: K
authors.lastname: Torlińska
authors.forename: A
authors.initials: A
authors.lastname: Grochowalska
authors.forename: J
authors.initials: J
authors.lastname: Kupsz
authors.forename: J
authors.initials: J
authors.lastname: Skoracka
authors.forename: S
authors.initials: S
authors.lastname: Kojo
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000367:Age Factors; D000818:Animals; D001921:Brain / Q000201:enzymology*; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D006943:Hyperglycemia / Q000097:blood / Q000201:enzymology*; D006946:Hyperinsulinism / Q000097:blood / Q000201:enzymology; D007328:Insulin / Q000097:blood; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013574:Synaptosomes / Q000201:enzymology*
chemical_list: D007328:Insulin; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase