title: Adrenergic receptor density in brown adipose tissue of active and hibernating hamsters and ground squirrels.
abstract: The ligand-binding characteristics (B(max) and K(D)) of alpha(1)- and beta(1)/beta(2)-adrenoceptors were investigated in membranes prepared from brown adipose tissue (BAT) of warm-acclimated, cold-acclimated, hibernating and arousing ground squirrels (Spermophillus undulatus) and hamsters (Mesocricetus auratus) by specific binding of [(3)H]prazosin and [(3)H]CGP-12177, respectively. The physiological state did not change the affinity for the adrenoceptors in the BAT of ground squirrels and hamsters. There was a significant decrease in alpha(1)-receptor density in arousing ground squirrels and a significant decrease in beta(1)/beta(2) density in hibernating ground squirrels. The level of alpha(1)-receptors was in all conditions higher than that of beta(1)/beta(2) receptors. The results indicate a possible change in balance of adrenoceptor density in the processes of cold acclimation, hibernation and arousal. The balance between the various adrenoceptor subtypes may be important for the final effect of catecholamines in BAT in different physiological states.
authors.affiliation: The Wenner-Gren Institute, The Arrhenius Laboratories F3, Stockholm University, SE-106 91 Stockholm, Sweden. lkramarova@rambler.ru
authors.forename: Ludmila I
authors.initials: LI
authors.lastname: Kramarova
authors.forename: Gennady E
authors.initials: GE
authors.lastname: Bronnikov
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Ignat'ev
authors.forename: Barbara
authors.initials: B
authors.lastname: Cannon
authors.forename: Jan
authors.initials: J
authors.lastname: Nedergaard
mesh_terms: D000064:Acclimatization; D002001:Adipose Tissue, Brown / Q000737:chemistry* / Q000502:physiology; D000818:Animals; D001143:Arousal / Q000502:physiology; D003080:Cold Temperature; D006224:Cricetinae; D005260:Female; D006605:Hibernation / Q000502:physiology*; D008297:Male; D008647:Mesocricetus / Q000502:physiology*; D011224:Prazosin / Q000378:metabolism; D011412:Propanolamines / Q000378:metabolism; D011941:Receptors, Adrenergic / Q000032:analysis*; D018340:Receptors, Adrenergic, alpha-1 / Q000032:analysis; D018342:Receptors, Adrenergic, beta-1 / Q000032:analysis; D018343:Receptors, Adrenergic, beta-2 / Q000032:analysis; D012589:Sciuridae / Q000502:physiology*
chemical_list: D011412:Propanolamines; D011941:Receptors, Adrenergic; D018340:Receptors, Adrenergic, alpha-1; D018342:Receptors, Adrenergic, beta-1; D018343:Receptors, Adrenergic, beta-2; C033394:CGP 12177; D011224:Prazosin