Up-regulation of fibroblast growth factor 3 is associated with tumor metastasis and recurrence in human hepatocellular carcinoma.
Recently, we established a hepatocellular carcinoma (HCC) cell line (named H4-M) from a metastatic HCC tumor. In H4-M, a marker chromosome containing a homogeneously staining region (hsr) was identified by cytogenetic analysis. The hsr was characterized by chromosome microdissection and the result showed that the hsr was composed of DNA sequence from 11q13. Oncogenes CCND1 and FGF3 were localized within the complicon and overexpressions of CCND1 and FGF3 were confirmed by Northern blot analysis. Clinical significance of FGF3 overexpression was studied by immunohistochemistry (IHC) using an HCC tissue microarray (TMA) containing 60 pairs of primary/metastatic HCCs and 30 pairs of primary/recurrent HCCs. TMA study showed that overexpression of FGF3 was significantly associated with HCC metastasis and recurrence (p<0.01), suggesting that up-regulation of FGF3 may play an important role in HCC metastasis and recurrence.
Hu
Liang
L
Department of Clinical Oncology, The University of Hong Kong, Pokfulam, Hong Kong, China.
Sham
Jonathan S T
JS
Xie
Dan
D
Wen
Jian-Ming
JM
Wang
Wei-Sheng
WS
Wang
Yi
Y
Guan
Xin-Yuan
XY
D015152:Blotting, Northern; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism / Q000556:secondary*; D045744:Cell Line, Tumor; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics; D056741:Cyclin D; D016213:Cyclins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D051518:Fibroblast Growth Factor 3 / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D007621:Karyotyping; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D005810:Multigene Family / Q000235:genetics; D012008:Recurrence; D046888:Tissue Array Analysis; D015854:Up-Regulation
D056741:Cyclin D; D016213:Cyclins; C496444:FGF3 protein, human; D051518:Fibroblast Growth Factor 3