Insertion polymorphism of transposable elements and population structure of Anopheles gambiae M and S molecular forms in Cameroon.
The insertion polymorphism of five transposable element (TE) families was studied by Southern blots in several populations of the M and S molecular forms of the mosquito Anopheles gambiae sensu stricto from southern Cameroon. We showed that the mean TE insertion site number and the within-population insertion site polymorphism globally differed between the M and S molecular forms. The comparison of the TE insertion profiles of the populations revealed a significant differentiation between these two molecular forms (0.163 < Phi(ST) < 0.371). We cloned several insertions of a non-LTR retrotransposon (Aara8) that were fixed in one form and absent in the other one. The only insertion that could be clearly located on a chromosome arm mapped to cytological division 6 of chromosome X, confirming the importance of this region in the ongoing speciation between the M and S molecular forms.
Boulesteix
M
M
Laboratoire de Biométrie et Biologie Evolutive, UMR 5558, CNRS, Université Claude Bernard Lyon1, 69622 Villeurbanne Cedex, France.
Simard
F
F
Antonio-Nkondjio
C
C
Awono-Ambene
H P
HP
Fontenille
D
D
Biémont
C
C
D000818:Animals; D000852:Anopheles / Q000145:classification / Q000235:genetics*; D015139:Blotting, Southern; D002163:Cameroon; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D017931:DNA Primers; D004251:DNA Transposable Elements / Q000235:genetics*; D005828:Genetics, Population; D011110:Polymorphism, Genetic
D017931:DNA Primers; D004251:DNA Transposable Elements