Immunolocalization of the PmSUC1 sucrose transporter in Plantago major flowers and reporter-gene analyses of the PmSUC1 promoter suggest a role in sucrose release from the inner integument.
This paper presents a detailed analysis of the PmSUC1 gene from plantago major, of its promoter activity in Arabidopsis, and of the tissue specific localization of the encoded protein in Plantago. PmSUC1 promoter activity was detected in the innermost layer of the inner integument (the endothel) of Arabidopsis plants expressing the gene of the green fluorescent protein (GFP) under the control of the PmSUC1 promoter. This promoter activity was confirmed with a PmSUC1-specific antiserum that identified the PmSUC1 protein in the endothel of Plantago and of Arabidopsis plants expressing the PmSUC1 gene under the control of its own promoter. PmSUC1 promoter activity and PmSUC1 protein were also detected in pollen grains during maturation inside the anthers and in pollen tubes during and after germination. These results demonstrate that PmSUC1 is involved in sucrose partitioning to the young embryo and to the developing pollen and growing pollen tube. In the innermost cell layer of the inner integument, a tissue that delivers nutrients to the endosperm and the embryo, PmSUC1 may catalyze the release of sucrose into the apoplast.
Lauterbach
C
C
Molekulare Pflanzenphysiologie, Universität Erlangen-Nürnberg, Staudtstrasse 5, 91058 Erlangen, Germany.
Niedermeier
M
M
Besenbeck
R
R
Stadler
R
R
Sauer
N
N
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D005091:Exons; D035264:Flowers / Q000235:genetics / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry; D007438:Introns; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D010943:Plantago / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011058:Pollen / Q000235:genetics / Q000378:metabolism; D053205:Pollen Tube / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D013395:Sucrose / Q000378:metabolism*; D014158:Transcription, Genetic
D026901:Membrane Transport Proteins; D010940:Plant Proteins; C078729:sucrose transport protein, plant; D049452:Green Fluorescent Proteins; D013395:Sucrose