The effect of centaurein on interferon-gamma expression and Listeria infection in mice.
We previously found that centaurein enhanced IFN-gamma transcription in T cells. Here, we demonstrate that centaurein increased the IFN-gamma expression in T and NK cells and the serum IFN-gamma level in mice. Centaurein elevated the transcription of T-bet but not GATA-3, which is consistent with its effect on that of IFN-gamma but not IL-4. Additionally, centaurein effectively protected mice against Listeria infection. Moreover, centaurein per se or in combination with antibiotics could treat Listeria infection. Our mechanistic studies suggest that centaurein augments IFN-gamma expression via a transcriptional up-regulation of T-bet and that centaurein protects against or treats Listeria infection via a modulation of IFN-gamma expression.
Chang
Shu-Lin
SL
Agricultural Biotechnology Research Center, 128, Sec. 2, Academia Road, Nankang, Taipei 115, Taiwan.
Yeh
Hsu-Hua
HH
Lin
Yu-Shiun
YS
Chiang
Yi-Ming
YM
Wu
Tung-Kung
TK
Yang
Wen-Chin
WC
D000818:Animals; D031176:Bidens / Q000737:chemistry; D018274:Electroporation; D005419:Flavonoids / Q000494:pharmacology*; D005960:Glucosides / Q000494:pharmacology*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D007694:Killer Cells, Natural / Q000187:drug effects / Q000378:metabolism; D008088:Listeriosis / Q000188:drug therapy* / Q000382:microbiology; D008156:Luciferases / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008519:Medicine, Traditional; D051379:Mice; D008810:Mice, Inbred C57BL; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D013268:Stimulation, Chemical; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism
D005419:Flavonoids; D005960:Glucosides; C111521:centaurein; D007371:Interferon-gamma; D008156:Luciferases