[Effects of inactivated rabbit serum containing compound realgar and natural indigo tablet on cell line NB4].
OBJECTIVE
To explore the effects of inactivated rabbit serum containing compound realgar and natural indigo tablet (CRNIT) on cell line NB(4).


METHODS
The experimental rabbits were taken as the provider of the animal serum, and the serum was inactivated before the experiment. The serum was divided into two groups based on whether the rabbits were given CRNIT. The concentration of arsenic in the rabbit's serum was detected by AFS-230a double path atom fluorescence photometer. The inhibition rates and apoptosis rates were regarded as the observational indexes.


RESULTS
The concentration of arsenic in the inactivated rabbit serum containing and not containing the drug were (0.010 0+/-0.001 0) mg/L and (0.110 0+/-0.006 4) mg/L respectively, and the difference had statistical significance (P<0.01). The two groups of serum all had inhibitory effect on the growth of NB(4) cells depending on the drug concentration and effect time. And there were significant differences among the groups. The two groups of serum all induced the apoptosis of NB(4) with positive relations with the concentration and effect time. And there were significant differences among the groups.


CONCLUSIONS
The rabbit serum containing CRNIT can obviously restrain the growth of NB(4) cells and the inhibitory effect depends on the concentration and effect time. And the rabbit serum containing CRNIT can also induce the apoptosis of NB(4) cell line and the apoptosis rates depend on the concentration and effect time.
Chen
Nan-nan
NN
Graduate School of General Hospital of Peopleos Liberation ArmyìBeijing 100853, China.
Huang
Shi-lin
SL
Xiang
Yang
Y
Zhang
De-jie
DJ
Guo
Ai-xia
AX
Chen
Ai-ping
AP
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001152:Arsenicals / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D007203:Indigo Carmine; D007211:Indoles / Q000494:pharmacology*; D008297:Male; D011817:Rabbits; D044967:Serum / Q000737:chemistry; D013440:Sulfides / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D013607:Tablets
D000970:Antineoplastic Agents; D001152:Arsenicals; D007211:Indoles; D013440:Sulfides; D013607:Tablets; C058317:arsenic disulfide; D007203:Indigo Carmine