[Studies on the interaction between thionine and deoxyribo-nucleic acid by ethidium bromide probe].
The interaction between thionine and deoxyribonucleic acid was studied with ethidium bromide probe in Tris-HCl buffer solution at pH 7.4 by fluorescence spectra, electronic absorption spectra, and resonance light scattering spectra. From the obtained results, it was found obviously that the planar structure of thionine can be intercalated into the stacked base pairs of DNA, which is the major controlling factor. The binding constant was found to be 1.0 x 10(5) L x mol(-1).
Liu
Xue-ping
XP
College of Chemistry and Environmental Science, Henan Normal University, Xinxiang 453007, China.
Feng
Su-ling
SL
Pan
Zi-hong
ZH
Fan
Jing
J
D004247:DNA / Q000737:chemistry*; D004996:Ethidium / Q000737:chemistry*; D010640:Phenothiazines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D010640:Phenothiazines; D004247:DNA; D004996:Ethidium; C009469:thionine