title: Goat PRND expression pattern suggests its involvement in early sex differentiation.
abstract: Expression of the goat prion protein gene locus was assessed by reverse transcriptase-polymerase chain reaction on testes and ovaries at various developmental stages. A weak and stochastic expression of the PRNP and PRNT genes was observed. For PRNT, it is consistent with the detected deletions of two single nucleotides within its open reading frame in ruminant genes. PRND was expressed in both tissues at all stages. Whereas its expression is constant in the ovaries, it increases in testes between 36 and 46 days postcoitum (dpc) and remains high thereafter. In testes, Doppel was found in the nucleus of germinal cells and in the cytoplasm of Leydig cells at 44 dpc. It was detected in the cytoplasm of Leydig cells and of some Sertoli and germinal cells at 62 dpc. In the ovaries, it was observed in the nucleus of germinal cells at 44 dpc and mainly in their cytoplasm at 62 dpc. This expression pattern was shown to parallel that of C-kit and suggests Doppel involvement in early testis differentiation.
authors.affiliation: UMR de Biologie du Développement et Reproduction, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
authors.forename: A
authors.initials: A
authors.lastname: Kocer
authors.forename: M
authors.initials: M
authors.lastname: Gallozzi
authors.forename: L
authors.initials: L
authors.lastname: Renault
authors.forename: G
authors.initials: G
authors.lastname: Tilly
authors.forename: I
authors.initials: I
authors.lastname: Pinheiro
authors.forename: F
authors.initials: F
authors.lastname: Le Provost
authors.forename: E
authors.initials: E
authors.lastname: Pailhoux
authors.forename: J L
authors.initials: JL
authors.lastname: Vilotte
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006041:Goats / Q000196:embryology / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008969:Molecular Sequence Data; D010053:Ovary / Q000737:chemistry / Q000196:embryology / Q000378:metabolism; D011247:Pregnancy; D011328:Prions / Q000235:genetics* / Q000378:metabolism; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012733:Sex Differentiation / Q000235:genetics*; D013737:Testis / Q000737:chemistry / Q000196:embryology / Q000378:metabolism
chemical_list: D011328:Prions; D019009:Proto-Oncogene Proteins c-kit