Isolation and characterization of 1,2,4-trichlorobenzene mineralizing Bordetella sp. and its bioremediation potential in soil.
A soil which has been polluted with chlorinated benzenes for more than 25 years was used for isolation of adapted microorganisms able to mineralize 1,2,4-trichlorobenzene (1,2,4-TCB). A microbial community was enriched from this soil and acclimated in liquid culture under aerobic conditions using 1,2,4-TCB as a sole available carbon source. From this community, two strains were isolated and identified by comparative sequence analysis of their 16S-rRNA coding genes as members of the genus Bordetella with Bordetella sp. QJ2-5 as the highest homological strain and with Bordetella petrii as the closest related described species. The 16S-rDNA of the two isolated strains showed a similarity of 100%. These strains were able to mineralize 1,2,4-TCB within two weeks to approximately 50% in liquid culture experiments. One of these strains was reinoculated to an agricultural soil with low native 1,2,4-TCB degradation capacity to investigate its bioremediation potential. The reinoculated strain kept its biodegradation capability: (14)C-labeled 1,2,4-TCB applied to this inoculated soil was mineralized to about 40% within one month of incubation. This indicates a possible application of the isolated Bordetella sp. for bioremediation of 1,2,4-TCB contaminated sites.
Wang
Fang
F
Institute of Soil Ecology, GSF - National Research Center for Environment and Health, 85764 Neuherberg, Germany.
Grundmann
Sabine
S
Schmid
Michael
M
Dörfler
Ulrike
U
Roherer
Stefanie
S
Charles Munch
Jean
J
Hartmann
Anton
A
Jiang
Xin
X
Schroll
Reiner
R
D001673:Biodegradation, Environmental; D001884:Bordetella / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002722:Chlorobenzenes / Q000378:metabolism*; D004269:DNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
D002722:Chlorobenzenes; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; C009947:1,2,4-trichlorobenzene