GRA1 protein vaccine confers better immune response compared to codon-optimized GRA1 DNA vaccine.
The present study evaluates immunogenicity and protection potency of a codon-optimized GRA1 DNA vaccine, wild type GRA1 DNA vaccine and an adjuvanted recombinant GRA1 protein vaccine candidate in BALB/c mice against lethal toxoplasmosis. Of the three GRA1 vaccines tested, the recombinant GRA1 protein vaccine results reveal significant increase in immune response and prolonged survival against acute toxoplasmosis compared to DNA vaccinations. Immune response and protection conferred by codon-optimized GRA1 DNA vaccine was slightly better than wild type GRA1 DNA vaccine.
Döşkaya
Mert
M
Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA. jurnak@uci.edu
Kalantari-Dehaghi
Mina
M
Walsh
Craig M
CM
Hiszczyńska-Sawicka
Elzbieta
E
Davies
D Huw
DH
Felgner
Philip L
PL
Larsen
Liza S Z
LS
Lathrop
Richard H
RH
Hatfield
G Wesley
GW
Schulz
Jessica R
JR
Gürüz
Yüksel
Y
Jurnak
Frances
F
D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D004195:Disease Models, Animal; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000276:immunology; D014123:Toxoplasmosis / Q000276:immunology / Q000517:prevention & control*; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology*; D022223:Vaccines, Subunit / Q000276:immunology*
D000953:Antigens, Protozoan; C412913:GRA1 antigen; D011994:Recombinant Proteins; D019444:Vaccines, DNA; D022223:Vaccines, Subunit