Characterization of Plasmodium vivax heat shock protein 70 and evaluation of its value for serodiagnosis of tertian malaria.
We have characterized Plasmodium vivax heat shock protein 70 (PvHSP70) and evaluated serodiagnostic applicability of recombinant PvHSP70 (rPvHSP70). In enzyme-linked immunosorbent assays and immunoblot analyses, rPvHSP70 showed high sensitivity (88.8%; 203/228 cases). P. falciparum-infected sera revealed positive reactions (78.8%). The predominant immunoglobulin G (IgG) subclasses were segregated with IgG1 and IgG3.
Na
Byoung-Kuk
BK
Department of Molecular Parasitology, Sungkyunkwan University School of Medicine, Suwon 440-746, Korea.
Park
Jae-Won
JW
Lee
Hyeong-Woo
HW
Lin
Klin
K
Kim
Seon-Hee
SH
Bae
Young-An
YA
Sohn
Woon-Mok
WM
Kim
Tong-Soo
TS
Kong
Yoon
Y
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D001483:Base Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000145:classification; D008288:Malaria / Q000175:diagnosis*; D008969:Molecular Sequence Data; D010966:Plasmodium vivax / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D012680:Sensitivity and Specificity; D012698:Serologic Tests
D000913:Antibodies, Protozoan; D018840:HSP70 Heat-Shock Proteins; D007074:Immunoglobulin G; D011994:Recombinant Proteins