[Cytotoxic effects of three kinds of eye drops for Chinese herbal medicines on various cell lines in vitro].
OBJECTIVE
To study the cytotoxic effects of three kinds of eye drops on human cancer cells and normal cells.


METHODS
Cytotoxic effects of Yuxingcao Eye Drop (YXC), Gegensu Eye Drop (GGS) and Shuanghuanglian Eye Drop (SHL) on 6 cell lines (CNE2, Glc-82, HRPE, Fibro, 3T3 and ECV-304) were assayed using MTF method. And cytotoxic efficacy was evaluated using fifty percent of inhibitory concentration (IC50).


RESULTS
IC50 of YXC to human cancer cells CNE2 and Glc-82 were (11.5 +/- 0.25) microl/ml and (24.0 +/- 0.8) microl/ml; to normal cells HRPE, Fibro, 3T3 and ECV-304 were (18.0 +/- 3.5) microl/ml, (52.0 +/- 14.0) microl/ml, (17.5 +/- 3.5) microl/ml and (17.5 +/- 1.3) microl/ml, respectively. IC50 of GGS to CNE2, Glc-82, ECV-304 cells were (9.8 +/- 2.3) microl/ml, (17.0 +/- 5.0) microl/ml and (10.5 +/- 0.95) microl/ml, respectively. Under the concentration of 100 microl/ml, the average survival rates of HRPE, Fibro and 3T3 cells were 60.0%, 87.8% and 58.2%, respectively. IC50 of SHL to CNE2, Glc-82 and HRPE cells were (18.9 +/- 5.0) microl/ ml, (23.9 +/- 0.6) micorl/ml and (113.9 +/- 25.6) microl/ml. At the concentration of 100 microl/ml, the average survival rates of Fibro, 3T3 and ECV-304 Cells were 89.6%, 77.2% and 74.7%.


CONCLUSIONS
YXC has evident cytotoxicities to human cancer cells and normal cells. While, GGS and SHL has not obvious cytotoxic effects to normal cells.
Li
Yu
Y
Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou 510060, China.
He
Mei-feng
MF
Huang
Chu-long
CL
D016475:3T3 Cells; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects; D006801:Humans; D051379:Mice; D009883:Ophthalmic Solutions / Q000145:classification / Q000494:pharmacology*; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000187:drug effects*; D010946:Plants, Medicinal / Q000737:chemistry
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D009883:Ophthalmic Solutions