title: Mapping of MYC breakpoints in 8q24 rearrangements involving non-immunoglobulin partners in B-cell lymphomas.
abstract: Chromosomal translocations joining the immunoglobulin (IG) and MYC genes have been extensively reported in Burkitt's and non-Burkitt's lymphomas but data concerning MYC rearrangements with non-IG partners are scarce. In this study, 8q24 breakpoints from 17 B-cell lymphomas involving non-IG loci were mapped by fluorescence in situ hybridization (FISH). In seven cases the breakpoint was inside a small region encompassing MYC: in one t(7;8)(p12;q24) and two t(3;8)(q27;q24), it was telomeric to MYC whereas in four cases, one t(2;8)(p15;q24) and three t(8;9)(q24;p13) it was located in a 85 kb region encompassing MYC. In these seven cases, partner regions identified by FISH contained genes known to be involved in lymphomagenesis, namely BCL6, BCL11A, PAX5 and IKAROS. Breakpoints were cloned in two t(8;9)(q24;p13), 2.5 and 7 kb downstream from MYC and several hundred kb 5' to PAX5 on chromosome 9, joining MYC to ZCCHC7 and to ZBTB5 exon 2, two genes encoding zinc-finger proteins. In these seven cases, MYC expression measured by quantitative reverse transcription-polymerase chain reaction (RT-PCR) was significantly higher when compared to that of patients without 8q24 rearrangement (P=0.006). These results suggest that these rearrangements are the consequence of a non-random process targeting MYC together with non-IG genes involved in lymphocyte differentiation and lymphoma progression.
authors.affiliation: Groupe d'Etude des Proliférations Lymphoïdes, Centre Henri Becquerel, INSERM U614, IFRMP23, Rouen, France. pbertrand@rouen.fnclcc.fr
authors.forename: P
authors.initials: P
authors.lastname: Bertrand
authors.forename: C
authors.initials: C
authors.lastname: Bastard
authors.forename: C
authors.initials: C
authors.lastname: Maingonnat
authors.forename: F
authors.initials: F
authors.lastname: Jardin
authors.forename: C
authors.initials: C
authors.lastname: Maisonneuve
authors.forename: M-N
authors.initials: MN
authors.lastname: Courel
authors.forename: P
authors.initials: P
authors.lastname: Ruminy
authors.forename: J-M
authors.initials: JM
authors.lastname: Picquenot
authors.forename: H
authors.initials: H
authors.lastname: Tilly
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D002051:Burkitt Lymphoma / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D019457:Chromosome Breakage; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics / Q000648:ultrastructure; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics / Q000648:ultrastructure; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics / Q000648:ultrastructure; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics* / Q000648:ultrastructure; D002899:Chromosomes, Human, Pair 9 / Q000235:genetics / Q000648:ultrastructure; D004268:DNA-Binding Proteins / Q000235:genetics; D005260:Female; D016259:Genes, myc; D006801:Humans; D051740:Ikaros Transcription Factor / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D016393:Lymphoma, B-Cell / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics; D051757:PAX5 Transcription Factor / Q000235:genetics; D051560:Proto-Oncogene Proteins c-bcl-6; D012097:Repressor Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014178:Translocation, Genetic / Q000235:genetics*
chemical_list: C486563:BCL11A protein, human; C084103:BCL6 protein, human; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; C497239:IKZF1 protein, human; D009687:Nuclear Proteins; D051757:PAX5 Transcription Factor; C497281:PAX5 protein, human; D051560:Proto-Oncogene Proteins c-bcl-6; D012097:Repressor Proteins; D051740:Ikaros Transcription Factor