title: Ectopic lymphoid neogenesis in psoriatic arthritis.
abstract: BACKGROUND
  Ectopic lymphoid neogenesis (LN) occurs in rheumatoid synovium, where it is thought to drive local antigen-dependent B cell development and autoantibody production. This process involves the expression of specific homing chemokines and the development of high endothelial venules (HEV).
  
  
  OBJECTIVE
  To investigate whether these mechanisms occur in psoriatic arthritis (PsA) synovium, where autoantibodies have not been described and the organisation and function of B cells is not clear, and to analyse their clinical correlates.
  
  
  METHODS
  Arthroscopic synovial biopsy specimens from patients with PsA before and after tumour necrosis factor alpha blockade were characterised by immunohistochemical analysis for T/B cell segregation, peripheral lymph node addressin (PNAd)-positive HEV, and the expression of CXCL13, CCL21 and CXCL12 chemokines in relation to the size of lymphoid aggregates.
  
  
  RESULTS
  Lymphoid aggregates of variable sizes were observed in 25 of 27 PsA synovial tissues. T/B cell segregation was often observed, and was correlated with the size of lymphoid aggregates. A close relationship between the presence of large and highly organised aggregates, the development of PNAd+ HEV, and the expression of CXCL13 and CCL21 was found. Large organised aggregates with all LN features were found in 13 of 27 tissues. LN in PsA synovitis was not related to the duration, pattern or severity of the disease. The synovial LN pattern remained stable over time in persistent synovitis, but a complete response to treatment was associated with a regression of the LN features.
  
  
  CONCLUSIONS
  LN occurs frequently in inflamed PsA synovial tissues. Highly organised follicles display the characteristic features of PNAd+ HEV and CXCL13 and CCL21 expression, demonstrating that the microanatomical bases for germinal centre formation are present in PsA. The regression of LN on effective treatment indicates that the pathogenic and clinical relevance of these structures in PsA merits further investigation.
authors.affiliation: Servicio de Reumatología, Hospital 12 de Octubre, 28041 Madrid, Spain.
authors.forename: Juan D
authors.initials: JD
authors.lastname: Cañete
authors.forename: Begoña
authors.initials: B
authors.lastname: Santiago
authors.forename: Tineke
authors.initials: T
authors.lastname: Cantaert
authors.forename: Raimon
authors.initials: R
authors.lastname: Sanmartí
authors.forename: Antonio
authors.initials: A
authors.lastname: Palacin
authors.forename: Raquel
authors.initials: R
authors.lastname: Celis
authors.forename: Eduard
authors.initials: E
authors.lastname: Graell
authors.forename: Beatriz
authors.initials: B
authors.lastname: Gil-Torregrosa
authors.forename: Dominique
authors.initials: D
authors.lastname: Baeten
authors.forename: José L
authors.initials: JL
authors.lastname: Pablos
mesh_terms: D000328:Adult; D018501:Antirheumatic Agents / Q000627:therapeutic use; D015535:Arthritis, Psoriatic / Q000150:complications* / Q000188:drug therapy / Q000276:immunology / Q000473:pathology; D001402:B-Lymphocytes / Q000473:pathology; D001706:Biopsy; D018925:Chemokines / Q000378:metabolism; D002828:Choristoma / Q000209:etiology* / Q000276:immunology / Q000473:pathology; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D042601:Lymphatic Vessels / Q000473:pathology; D008221:Lymphoid Tissue; D008297:Male; D008875:Middle Aged; D012720:Severity of Illness Index; D013583:Synovial Membrane / Q000276:immunology / Q000473:pathology*; D013601:T-Lymphocytes / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors
chemical_list: D018501:Antirheumatic Agents; D018925:Chemokines; D014409:Tumor Necrosis Factor-alpha