[RAPD analysis on the germplasm resources of Gastrodia elata in Guizhou].
Genetic polymorphisms of genomic DNA of 15 samples from wild and culturaled Gastrodia elata Blume in Guizhou were analyzed by RAPD method. 12 effective primers are screened from 40 primers amplified a total number of 93 loci, among which 66 are polymorphic and the percentage of polymorphic loci (PPB) is 70.97%. UPGMA dendrogram analyzed by NTSYSpc, ver. 2.2 shows that Gastrodia elata have apparent genetic variance. Geological distribution and growing environment were significant factors for the polymorphism.
Zou
Jia-ning
JN
Guiyang Medical Collge, Guiyang 550004, China.
Song
Ju-xian
JX
Chang
Chu-rui
CR
Wang
Xiao-li
XL
D002681:China; D016000:Cluster Analysis; D018744:DNA, Plant / Q000235:genetics*; D031668:Gastrodia / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D005819:Genetic Markers; D010802:Phylogeny; D010946:Plants, Medicinal / Q000235:genetics* / Q000254:growth & development; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA
D018744:DNA, Plant; D005819:Genetic Markers