Interaction of immobilized human exportin-t with calf liver tRNA.
The binding of tRNA species from calf liver to immobilized exportin-t in the presence of ran x GppNHp was examined by affinity chromatography. We observed different eluting behaviors of individual tRNAs. After separating tRNAs on a two-dimensional polyacrylamide gel, the positions of seven selected tRNAs were identified by Northern hybridization and their relative affinities to immobilized exportin-t x ran x GppNHp complex were estimated in the order of tRNA(Leu)(CAG) > tRNA(Ser)(GCU), tRNA(Leu)(CAA), tRNA(Ser)(UGA) > tRNA(Ser)(AGA), tRNA(Leu)(AAG) > tRNA(Arg)(ACG). We propose that exportin-t preferentially binds and exports those tRNAs that are rapidly consumed by the protein synthesis machinery.
Li
Sheng
S
Laboratorium für Biochemie, Universität Bayreuth, Bayreuth, Germany.
Sprinzl
Mathias
M
D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D006801:Humans; D008099:Liver / Q000378:metabolism*; D029543:Nucleocytoplasmic Transport Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding / Q000235:genetics; D012343:RNA, Transfer / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
D029543:Nucleocytoplasmic Transport Proteins; C503618:XPOT protein, human; D012343:RNA, Transfer