title: The effect of ovariectomy on biomarkers of urogenital ageing in old versus young adult rats.
abstract: The aim of this study was to compare the effects of ageing and ovariectomy on biomarkers of urogenital ageing in old and young-adult rats. Fisher 344 rats (18- and 3-months-old, n = 6 x 2) underwent ovariectomy. Age-matched sham animals received no intervention (n = 6 x 2). One month later, biomarkers of urogenital ageing were evaluated (light microscopic count of urethral and anal canal submucosal blood vessels, Western blot analysis of urethral, and anal canal submucosal collagen I and III and cytoplasmic p27(kip1) expression in the striated urethral and anal sphincters and levator ani and gel electrophoresis of isomyosin I proportion in these muscles) and compared in all groups (n = 24). All biomarkers of urogenital ageing studied were significantly increased in old compared to young-adult sham rats. Ovariectomy significantly increased these changes further in old versus young-adult rats with either smaller or larger differential effect than ageing compared to young-adult sham animals. Ovariectomy significantly exacerbates normative urogenital ageing changes in rats.
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666, Al-Ain, United Arab Emirates. rizk.diaa@uaeu.ac.ae
authors.forename: Diaa E E
authors.initials: DE
authors.lastname: Rizk
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Fahim
authors.forename: Hazem A
authors.initials: HA
authors.lastname: Hassan
authors.forename: Ahmed H
authors.initials: AH
authors.lastname: Al-Marzouqi
authors.forename: Gaber A
authors.initials: GA
authors.lastname: Ramadan
authors.forename: Soha S
authors.initials: SS
authors.lastname: Al-Kedrah
authors.forename: Layla S
authors.initials: LS
authors.lastname: Al-Ghafri
mesh_terms: D000375:Aging / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D024042:Collagen Type I / Q000378:metabolism*; D024061:Collagen Type III / Q000378:metabolism*; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000378:metabolism*; D004967:Estrogens / Q000378:metabolism; D005260:Female; D018482:Muscle, Skeletal / Q000378:metabolism / Q000473:pathology; D010052:Ovariectomy; D017773:Pelvic Floor / Q000473:pathology; D051381:Rats; D011916:Rats, Inbred F344; D014521:Urethra / Q000098:blood supply* / Q000378:metabolism* / Q000473:pathology
chemical_list: D015415:Biomarkers; D024042:Collagen Type I; D024061:Collagen Type III; D004967:Estrogens; D050760:Cyclin-Dependent Kinase Inhibitor p27