title: [Sulfate reduction and methanogenesis in the Shira and Shunet meromictic lakes (Khakass Republic, Russia)].
abstract: The biogeochemical and molecular biological study of the chemocline and sediments of saline meromictic lakes Shira and Shunet (Khakass Republic, Russia) was performed. A marked increase in the rates of sulfate reduction and methanogenesis was revealed at the medium depths of the chemocline. The rates of these processes in the bottom sediments decreased with depth. The numbers of Bacteria, Archaea, and of sulfate-reducing bacteria (SRB) were determined by fluorescence in situ hybridization with rRNA specific oligonucleotide probes labeled with horseradish peroxidase and subsequent tyramide signal amplification. In the chemocline, both the total microbial numbers and those of Bacteria were shown to increase with depth. The archaea and SRB were present in almost equal numbers. In the lake sediments, a drastic decrease in microbial numbers with depth was revealed. SRB were found to prevail in the upper sediment layer and archaea in the lower one. This finding correlates with the measured rates of sulfate reduction and methanogenesis.
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Kallistova
authors.forename: M V
authors.initials: MV
authors.lastname: Kevbrina
authors.forename: N V
authors.initials: NV
authors.lastname: Pimenov
authors.forename: I I
authors.initials: II
authors.lastname: Rusanov
authors.forename: D Iu
authors.initials: DIu
authors.lastname: Rogozin
authors.forename: B
authors.initials: B
authors.lastname: Wehrli
authors.forename: A N
authors.initials: AN
authors.lastname: Nozhevnikova
mesh_terms: D001105:Archaea / Q000302:isolation & purification* / Q000378:metabolism; D001419:Bacteria / Q000302:isolation & purification* / Q000378:metabolism; D005618:Fresh Water / Q000737:chemistry / Q000382:microbiology*; D019015:Geologic Sediments / Q000382:microbiology; D008697:Methane / Q000096:biosynthesis*; D015345:Oligonucleotide Probes; D020401:Primed In Situ Labeling; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012800:Siberia; D013431:Sulfates / Q000378:metabolism*; D014871:Water Microbiology
chemical_list: D015345:Oligonucleotide Probes; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D013431:Sulfates; D008697:Methane