Detection of hepatitis B virus DNA by in situ tissues hybridization in corneal of HBsAG positive donors.
HBV is a hepatotropic virus. The highest concentration found in the blood and liver with lower amount in saliva and semen. The virus was also detected in body fluids. Keratoplasty is an essential operation for the treatment of corneal blindness. At Ain Shams University International Eye Bank 10% of the collected corneas were from donors with HBs antigenemia. These corneas were rejected according to the Eye Bank Association of America. In this study 32 corneal tissues of 16 donors positive HBsAg were examined for,the presence of HBV by in situ DNA hybridization technique to detect HBV DNA in the corneal sections. This marker could not be seen in this work. This preliminary study could be an encouraging step for further researches to evaluate the possibility of the avascular cornea to carry HBV.
Helmy
M F
MF
Microbiology Department, Faculty of Medicine, Ain Shams University.
Ahmed
H M
HM
Saber
M A
MA
D003315:Cornea / Q000821:virology*; D016039:Corneal Transplantation / Q000009:adverse effects / Q000706:statistics & numerical data; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D046148:Donor Selection; D004534:Egypt / Q000453:epidemiology; D005125:Eye Banks; D006509:Hepatitis B / Q000097:blood / Q000453:epidemiology / Q000635:transmission / Q000821:virology; D006514:Hepatitis B Surface Antigens / Q000097:blood*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D020287:In Situ Nick-End Labeling; D012680:Sensitivity and Specificity; D014019:Tissue Donors / Q000706:statistics & numerical data
D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens