title: [RHD 1227A allele frequency among Rh negative population and random population].
abstract: To investigate the frequency of RHD 1227A allele in Rh negative population and random population, an AS-PCR (allele specific-polymerase chain reaction) method was employed to detect RHD 1227A allele. RHD gene copy was determined by D zygosity test and RHD exon 9 nucleotide sequence analysis. The results showed that among 143 Rh negative donors, forty-one RHD 1227A allele carriers were detected, and 8 (19.51%) out of which were RhCCdee, 32 (78.05%) were RhCcdee, and 1 (2.44%) was RhCcdEe. Thirty-five Rh negative RHD 1227A carriers had RHD gene deletion, and the remaining carriers were RHD 1227A homozygous. Seven (1.43%) individuals were detected with RHD 1227A allele among 489 random donors. They were all G/A heterozygous at RHD 1227 site. Serological test indicated that they were normal Rh positive phenotype. It is concluded that the frequency of RHD 1227A allele is 16.43% among Rh negative population and 0.72% among the random population.
authors.affiliation: Institute of Transfusion Medicine, Blood Center of Zhejiang Province; Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou 310006, China.
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Wu
authors.forename: Xiao-Zhen
authors.initials: XZ
authors.lastname: Hong
authors.forename: Xian-Guo
authors.initials: XG
authors.lastname: Xu
authors.forename: Kai-Rong
authors.initials: KR
authors.lastname: Ma
authors.forename: Fa-Ming
authors.initials: FM
authors.lastname: Zhu
authors.forename: Li-Xing
authors.initials: LX
authors.lastname: Yan
mesh_terms: D044466:Asian People / Q000235:genetics; D001483:Base Sequence; D002681:China; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D017353:Gene Deletion; D005787:Gene Frequency / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D012204:Rh-Hr Blood-Group System / Q000235:genetics* / Q000276:immunology; D017422:Sequence Analysis, DNA
chemical_list: D012204:Rh-Hr Blood-Group System; C026410:Rho(D) antigen