Effects of dietary energy intake and cold exposure on kinetics of plasma glucose metabolism in sheep.
An isotope dilution method using [U-(13)C]glucose injection was applied to determine the effects of dietary energy intake and cold exposure on plasma glucose metabolism in sheep. The sheep were assigned to two dietary treatments and were fed on diets containing either 100% or 160% of ME and both containing 150% of dietary crude protein intake for maintenance. The sheep were exposed from a thermoneutral environment (23 degrees C) to a cold environment (2-4 degrees C) for 5 days. The isotope dilution method was performed on the 18th day in the thermoneutral environment and on the fifth day of cold exposure. Plasma concentrations of glucose and NEFA increased (p < 0.05) during cold exposure for both diets. Plasma glucose pool size remained unchanged (p = 0.67), but plasma glucose turnover rate tended to increase (p = 0.07) with increased energy intake. Both pool size and turnover rate of plasma glucose increased (p = 0.01 and p = 0.0001, respectively) during cold exposure. No significant diet x environment interaction was detected. It is concluded that plasma glucose metabolism was influenced by both dietary energy intake and cold exposure, and plasma glucose metabolism in response to cold exposure was not modified by energy intake in sheep under the conditions (2-4 degrees C on the fifth day) of the present experiment.
Sano
H
H
Faculty of Agriculture, Iwate University, Morioka, Japan. sano@iwate-u.ac.jp
Sawada
H
H
Takenami
A
A
Oda
S
S
Al-Mamun
M
M
D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D002247:Carbon Isotopes; D003080:Cold Temperature; D018592:Cross-Over Studies; D004044:Dietary Proteins / Q000008:administration & dosage / Q000378:metabolism; D002149:Energy Intake / Q000502:physiology*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D008297:Male; D012756:Sheep / Q000097:blood / Q000378:metabolism / Q000502:physiology*
D001786:Blood Glucose; D002247:Carbon Isotopes; D004044:Dietary Proteins; D005230:Fatty Acids, Nonesterified