Activity based fingerprinting of proteases using FRET peptides.
We have successfully developed a protease assay using fluorescence resonance energy transfer based peptide libraries, which allows not only general detection of enzymatic activities, but more importantly substrate fingerprinting of proteases from different classes. The method allows the generation of substrate fingerprints of a protease from both the nonprime and prime sites. Therefore, it is well suited for profiling of major metalloproteases such as thermolysin and MMPs. We envisage that this method will provide a useful tool in the emerging field of Catalomics for high-throughput studies of proteases.
Sun
Hongyan
H
Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore, Singapore.
Panicker
Resmi C
RC
Yao
Shao Q
SQ
D031541:Fluorescence Resonance Energy Transfer; D010447:Peptide Hydrolases / Q000737:chemistry* / Q000378:metabolism; D019151:Peptide Library; D010449:Peptide Mapping / Q000379:methods*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity
D019151:Peptide Library; D010455:Peptides; D010447:Peptide Hydrolases