Clinical usefulness of antibodies against Babesia gibsoni detected by ELISA with recombinant P50.
The clinical usefulness of antibodies against Babesia gibsoni detected by ELISA with recombinant P50 was examined in dogs in an area where B. gibsoni infection was endemic. Only 8 among 14 dogs with acute type B. gibsoni infection without a previous history of infection were positive. This high percentage of false-negative results is thought to be a weak point of ELISA as a diagnostic tool. However, 14 other anemic dogs with a confirmed history of B. gibsoni infection were all positive, thus confirming the higher sensitivity of ELISA in detecting a history of infection.
Miyama
Takako
T
Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan.
Inokuma
Hisashi
H
Itamoto
Kazuhito
K
Okuda
Masaru
M
Verdida
Rodolfo A
RA
Xuan
Xuenan
X
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000276:immunology*; D001403:Babesia / Q000276:immunology*; D001404:Babesiosis / Q000097:blood / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D004283:Dog Diseases / Q000097:blood / Q000175:diagnosis* / Q000276:immunology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005188:False Negative Reactions
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; C434466:P50 protein, Babesia gibsoni