Residual polymerase activity-induced bias in terminal restriction fragment length polymorphism analysis.
Residual activity of polymerase chain reaction DNA polymerases in restriction analyses strongly affected genetic profiling based on terminal restriction fragment length polymorphisms. Artificial fragment sizes produced as a result of 5'-overhang restriction site fill-in and addition of a terminal A may bias genetic profiling and genotyping of microbial communities. Efficient removal of polymerases retained original fragment sizes and significantly reduced this profiling bias in soil bacterial communities.
Hartmann
Martin
M
Molecular Ecology, Agroscope Reckenholz-Tänikon Research Station ART, CH-8046 Zurich, Switzerland.
Enkerli
Jürg
J
Widmer
Franco
F
D001419:Bacteria / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase; D005838:Genotype; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D015203:Reproducibility of Results; D012988:Soil Microbiology
D004259:DNA-Directed DNA Polymerase