title: Optical and magnetic resonance imaging of cell death and platelet activation using annexin a5-functionalized quantum dots.
abstract: A quantum-dot-based nanoparticle is presented, allowing visualization of cell death and activated platelets with fluorescence imaging and MRI. The particle exhibits intense fluorescence and a large MR relaxivity (r1) of 3000-4500 mM-1 s-1 per nanoparticle due to a newly designed construct increasing the gadolinium-DTPA load. The nanoparticle is suitable for both anatomic and subcellular imaging of structures in the vessel wall and is a promising bimodal contrast agent for future in vivo imaging studies.
authors.affiliation: Department of Biophysics, University of Maastricht, and Department of Radiology, University Hospital Maastricht, The Netherlands. L.Prinzen@bf.unimaas.nl
authors.forename: Lenneke
authors.initials: L
authors.lastname: Prinzen
authors.forename: Robbert-Jan J H M
authors.initials: RJ
authors.lastname: Miserus
authors.forename: Anouk
authors.initials: A
authors.lastname: Dirksen
authors.forename: Tilman M
authors.initials: TM
authors.lastname: Hackeng
authors.forename: Niko
authors.initials: N
authors.lastname: Deckers
authors.forename: Nicole J
authors.initials: NJ
authors.lastname: Bitsch
authors.forename: Remco T A
authors.initials: RT
authors.lastname: Megens
authors.forename: Kim
authors.initials: K
authors.lastname: Douma
authors.forename: Johan W
authors.initials: JW
authors.lastname: Heemskerk
authors.forename: M Eline
authors.initials: ME
authors.lastname: Kooi
authors.forename: Peter M
authors.initials: PM
authors.lastname: Frederik
authors.forename: Dick W
authors.initials: DW
authors.lastname: Slaaf
authors.forename: Marc A M J
authors.initials: MA
authors.lastname: van Zandvoort
authors.forename: Chris P M
authors.initials: CP
authors.lastname: Reutelingsperger
mesh_terms: D017304:Annexin A5 / Q000737:chemistry*; D016923:Cell Death; D020285:Cryoelectron Microscopy; D019786:Gadolinium DTPA; D008279:Magnetic Resonance Imaging / Q000379:methods*; D053758:Nanoparticles; D055095:Optics and Photonics; D015539:Platelet Activation; D011789:Quantum Theory
chemical_list: D017304:Annexin A5; D019786:Gadolinium DTPA