title: GRA1 protein vaccine confers better immune response compared to codon-optimized GRA1 DNA vaccine.
abstract: The present study evaluates immunogenicity and protection potency of a codon-optimized GRA1 DNA vaccine, wild type GRA1 DNA vaccine and an adjuvanted recombinant GRA1 protein vaccine candidate in BALB/c mice against lethal toxoplasmosis. Of the three GRA1 vaccines tested, the recombinant GRA1 protein vaccine results reveal significant increase in immune response and prolonged survival against acute toxoplasmosis compared to DNA vaccinations. Immune response and protection conferred by codon-optimized GRA1 DNA vaccine was slightly better than wild type GRA1 DNA vaccine.
authors.affiliation: Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA. jurnak@uci.edu
authors.forename: Mert
authors.initials: M
authors.lastname: Döşkaya
authors.forename: Mina
authors.initials: M
authors.lastname: Kalantari-Dehaghi
authors.forename: Craig M
authors.initials: CM
authors.lastname: Walsh
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Hiszczyńska-Sawicka
authors.forename: D Huw
authors.initials: DH
authors.lastname: Davies
authors.forename: Philip L
authors.initials: PL
authors.lastname: Felgner
authors.forename: Liza S Z
authors.initials: LS
authors.lastname: Larsen
authors.forename: Richard H
authors.initials: RH
authors.lastname: Lathrop
authors.forename: G Wesley
authors.initials: GW
authors.lastname: Hatfield
authors.forename: Jessica R
authors.initials: JR
authors.lastname: Schulz
authors.forename: Yüksel
authors.initials: Y
authors.lastname: Gürüz
authors.forename: Frances
authors.initials: F
authors.lastname: Jurnak
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D004195:Disease Models, Animal; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000276:immunology; D014123:Toxoplasmosis / Q000276:immunology / Q000517:prevention & control*; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology*; D022223:Vaccines, Subunit / Q000276:immunology*
chemical_list: D000953:Antigens, Protozoan; C412913:GRA1 antigen; D011994:Recombinant Proteins; D019444:Vaccines, DNA; D022223:Vaccines, Subunit