Interaction of an organic selenium compound with human serum albumin: a spectroscopic study.
The interaction between 4,4'-diselenadibenzoic acid and human serum albumin (HSA) was investigated by fluorescence and absorption spectroscopy. The quenching mechanism of fluorescence of HSA by 4,4'-diselenadibenzoic acid was discussed. It is proved that the fluorescence quenching of HSA by 4,4'-diselenadibenzoic acid is a result of the formation of the HSA-4,4'-diselenadibenzoic acid complex. The binding sites number n, apparent corporation constant K, and corresponding thermodynamic parameters, deltaH(theta), deltaG(theta), and deltaS(theta) were calculated. Results indicate that the electrostatic interactions forces played major role in the reaction.
Sun
Shao-Fa
SF
Department of Chemistry and Life Sciences, Xianning College, Xianning 437005, People's Republic of China.
Pan
Ling-Li
LL
Wang
Cheng-Ming
CM
Hou
Han-Na
HN
Liu
Yi
Y
D006801:Humans; D016566:Organoselenium Compounds / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013816:Thermodynamics
C518383:4,4'-diselenadibenzoic acid; D016566:Organoselenium Compounds; D012709:Serum Albumin