Oceanicola nanhaiensis sp. nov., isolated from sediments of the South China Sea.
A Gram-negative, non-motile, rod-shaped bacterium, strain SS011B1-20(T), was isolated from sediments of the South China Sea. Growth occurred at NaCl concentrations between 0 and 10 % and at temperatures between 10 and 37 degrees C. Strain SS011B1-20(T) contained Q-10 as the major respiratory quinone and C(18 : 1)omega7c (81.2 %), C(16 : 0) (7.0 %) and C(18 : 1) methyl (4.3 %) as the predominant fatty acids. The G+C content of the genomic DNA was 64.7 mol%. A phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain SS011B1-20(T) belonged to a clade within the genus Oceanicola in the Alphaproteobacteria, the highest sequence similarities being found with respect to Oceanicola batsensis (96.3 %) and with Oceanicola granulosus (94.9 %). Strain SS011B1-20(T) could be clearly distinguished from other Oceanicola species on the basis of the genotypic, phenotypic and phylogenetic data. Thus, it is proposed that strain SS011B1-20(T) represents a novel species of the genus Oceanicola, with the name Oceanicola nanhaiensis sp. nov. The type strain is SS011B1-20(T) (=LMG 23508(T)=CGMCC 1.6293(T)).
Gu
Jun
J
Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, People's Republic of China.
Guo
Bin
B
Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, People's Republic of China.
Wang
Ya-Nan
YN
Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, People's Republic of China.
Yu
Su-Lin
SL
Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, People's Republic of China.
Inamori
Ryuhei
R
Graduate School of Life and Environmental Sciences, Tsukuba University, 1-1-1, Tennodai, Tsukuba 305-8572, Japan.
Qu
Ri
R
Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, People's Republic of China.
Ye
Yu-Guang
YG
Qingdao Institute of Marine Geology, Qingdao 266071, People's Republic of China.
Wu
Xiao-Lei
XL
Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, People's Republic of China.
D020561:Alphaproteobacteria / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S