[The effect of ligustrazine on cells proliferation in cortex and striatum after focal cerebral ischemia in adult rats].
OBJECTIVE
To explore the effect of Ligustrazine on cells proliferation in cortex and striatum after focal cerebral ischemia in adult rats.


METHODS
Focal cerebral ischemia was induced by left middle cerebral artery occlusion (MCAO) with suture method. Two hours later, injection of Ligustrazine (80 mg/kg, 1 time/d) was performed peritoneally. Four hours after the ischemia, 5-bromodeoxyuridine (BrdU) (50 mg/kg, 1 time/d) was injected peritoneally. At 7d, 14d and 21d after ischemia, BrdU-Labeled cells in the cortex and striatum were observed by immunohistochemical staining.


RESULTS
In ischemia model group, at 7 day, BrdU-labeled cells were observed in the ipsilateral cortex and striatum. With the prolongation of ischemia, the number of BrdU-labeled cells increased, reached the peak at 21d. In Ligustrazine group, BrdU-labeded cells were observed with an intense distribution in ischemic penumbra of the cortex and striatum. With the prolongation of ischemia, the number of BrdU-labeled cells increased significantly at 14d, and reached the peak at 21d. The numbers of BrdU-labeled cells at 7d, 14d and 21d were more than those in ischemia model group respectively.


CONCLUSIONS
Ligustrazine increase the proliferated cells in the cortex and striatum after focal cerebral ischemia in adult rats. The results suggest that it may be useful for promoting self-repair after ischemia.
Qiu
Fen
F
Research Center for Neuroscience, Department of Human Anatomy and Histological Embryology, Xi'an Jiaotong University, Xi'an 710061, China. qiufen6688@mail.xjtu.edu.cn
Liu
Yong
Y
Zhang
Peng-Bo
PB
Tian
Ying-Fang
YF
Zhao
Jian-Jun
JJ
Kang
Qian-Yan
QY
Qi
Cun-Fang
CF
Chen
Xin-Lin
XL
D000818:Animals; D019661:Apiaceae / Q000737:chemistry; D002545:Brain Ischemia / Q000188:drug therapy / Q000473:pathology*; D002452:Cell Count; D049109:Cell Proliferation / Q000187:drug effects*; D002540:Cerebral Cortex / Q000473:pathology; D003342:Corpus Striatum / Q000473:pathology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D020244:Infarction, Middle Cerebral Artery / Q000188:drug therapy / Q000473:pathology*; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry; D011719:Pyrazines / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D004365:Drugs, Chinese Herbal; D011719:Pyrazines; C017953:tetramethylpyrazine