A RGD-containing oligopeptide (K)16GRGDSPC: a novel vector for integrin-mediated targeted gene delivery.
A 23 amino acid, bifunctional integrin-targeted synthetic oligopeptide was evaluated for ex vivo gene delivery to rabbit bone marrow stromal cells (BMSCs). Synthesis of the peptide (K)16GRGDSPC was performed on a solid-phase batch peptide synthesizer. BMSCs were transfected with plasmid DNA coding for luciferase by (K)16GRGDSPC and the transfection efficiency was assayed. The influences of chloroquine and polyethyleneimine on the transfection efficiency were also examined. The target specificity of (K)16GRGDSPC to mediate exogenous gene into BMSCs was analyzed using cell attachment test and gene delivery inhibition test. The results showed that the transfection efficiency of the oligopeptide vector was lower than that of Lipofectamine. But in the presence of endosomal buffer chloroquine or endosomal disrupting agent polyethyleneimine, the transfection efficiency of the vector was greatly enhanced. In addition, RGD-containing peptides inhibited BMSCs' attachment to the 96-well plates pretreated with fibronectin or vitronectin and significantly decreased the transfection efficiency of the oligopeptide vector. These studies demonstrated that oligopeptide (K)16GRGDSPC was an ideal novel targeted non-viral gene delivery vector, which was easy to be synthesized, high efficient and low cytotoxicity. The vector could effectively deliver exogenous gene into rat BMSCs.
Pan
Haitao
H
Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Zheng
Qixin
Q
Guo
Xiaodong
X
Liu
Yong
Y
Li
Changwen
C
Song
Yulin
Y
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics*; D016023:Integrins / Q000235:genetics*; D008239:Lysine / Q000235:genetics; D009842:Oligopeptides / Q000235:genetics* / Q000378:metabolism; D011817:Rabbits; D017154:Stromal Cells / Q000166:cytology* / Q000378:metabolism; D014162:Transfection
D016023:Integrins; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D008239:Lysine