The role of FLO11 in Saccharomyces cerevisiae biofilm development in a laboratory based flow-cell system.
A role of the FLO11 in Saccharomyces cerevisiae biofilm development in a flow cell system was examined. We carried out an ectopic FLO11 expression in the wild type (wt) BY4741 strain that has low levels of endogenous FLO11 transcript. In contrast to the nonadhesive wt, the FLO11 overexpression strain (BY4741 FLO11(+)) readily adhered to both liquid-hydrophobic and liquid-hydrophilic solid interfaces and was able to grow as a biofilm monolayer in a flow system. Cellular features associated with FLO11 were examined and found to be consistent with the previous studies conducted in different strains of S. cerevisiae. When grown in suspended liquid culture, BY4741 FLO11(+) formed larger cellular aggregates (clumps), consisting of from five to 60 cells, and displayed an increased cell surface hydrophobicity, without changes in the cell size or growth rate, compared to wt. However, the invasive growth associated with FLO11 expression was not observed in BY4741 FLO11(+). The significance of these findings is discussed in the context of clinically and industrially relevant biofilms.
Purevdorj-Gage
Boloroo
B
Division of Health Sciences, Montana State University, Bozeman, MT 59717, USA.
Orr
Miranda E
ME
Stoodley
Paul
P
Sheehan
Kathy B
KB
Hyman
Linda E
LE
D018441:Biofilms / Q000254:growth & development*; D002449:Cell Aggregation / Q000502:physiology; D048429:Cell Size; D057927:Hydrophobic and Hydrophilic Interactions; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D012331:RNA, Fungal / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000502:physiology*; D014170:Transformation, Genetic / Q000502:physiology
C110690:FLO11 protein, S cerevisiae; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D012331:RNA, Fungal; D029701:Saccharomyces cerevisiae Proteins