Global genome expression analysis of rice in response to drought and high-salinity stresses in shoot, flag leaf, and panicle.
To elucidate genome-level responses to drought and high-salinity stress in rice, a 70 mer oligomer microarray covering 36,926 unique genes or gene models was used to profile genome expression changes in rice shoot, flag leaf and panicle under drought or high-salinity conditions. While patterns of gene expression in response to drought or high-salinity stress within a particular organ type showed significant overlap, comparison of expression profiles among different organs showed largely organ-specific patterns of regulation. Moreover, both stresses appear to alter the expression patterns of a significant number of genes involved in transcription and cell signaling in a largely organ-specific manner. The promoter regions of genes induced by both stresses or induced by one stress in more than one organ types possess relative enrichment of two cis-elements (ABRE core and DRE core) known to be associated with water stress. An initial computational analysis indicated that novel promoter motifs are present in the promoters of genes involved in rehydration after drought. This analysis suggested that rice might possess a mechanism that actively detects rehydration and facilitates rapid recovery. Overall, our data supports a notion that organ-specific gene regulation in response to the two abiotic stresses may primarily be mediated by organ-specific transcription responses.
Zhou
Junli
J
Peking-Yale Joint Center of Plant Molecular Genetics and Agrobiotechnology, College of Life Sciences, Peking University, Beijing, 100871, People's Republic of China.
Wang
Xiangfeng
X
Jiao
Yuling
Y
Qin
Yonghua
Y
Liu
Xigang
X
He
Kun
K
Chen
Chen
C
Ma
Ligeng
L
Wang
Jian
J
Xiong
Lizhong
L
Zhang
Qifa
Q
Fan
Liumin
L
Deng
Xing Wang
XW
D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics; D004190:Disasters; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000235:genetics* / Q000254:growth & development; D018515:Plant Leaves / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics; D018520:Plant Shoots / Q000502:physiology; D012045:Regulatory Sequences, Nucleic Acid; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012987:Soil / Q000032:analysis*; D014867:Water
D017931:DNA Primers; D018744:DNA, Plant; D010940:Plant Proteins; D012987:Soil; D014867:Water