Characterization of a hydroxyl-radical-producing glycoprotein and its presumptive genes from the white-rot basidiomycete Phanerochaete chrysosporium.
During wood decay, the white-rot basidiomycete Phanerochaete chrysosporium secretes low-molecular-mass glycoproteins that catalyze a redox reaction between O(2) and electron donors to produce hydroxyl radical. This reaction accounts for most of the hydroxyl radical produced in wood-degrading cultures of P. chrysosporium. In combination with phenol oxidases, hydroxyl radical is believed to play a role in lignin degradation. The secreted glycoproteins also reduce Fe(III) to Fe(II) and strongly bind Fe(II). The partially purified glycoproteins contain 1-amino-1-deoxy-2-ketose (ketoamine) produced by the condensation of side-chain amino groups and carbohydrate. cDNAs and two putative genes encoding these glycoproteins, glp1 and glp2, have been isolated and sequenced. The 875bp glp1 and 864bp glp2 are found on scaffold 2 of the P. chrysosporium genome. These presumptive genes each consist of seven introns and eight exons. The latter encode a predicted protein of 138 amino acids and a 22-amino-acid signal sequence for secretion. The predicted protein sequences are nearly identical to N-terminal and internal sequences obtained from the partially purified glycoprotein. The molecular masses of the deduced mature proteins, 13,981 (glp1) and 13,970 (glp2), coincide with the molecular mass of the glycoprotein as determined by tricine-SDS-PAGE.
Tanaka
Hiromi
H
Department of Applied Biological Chemistry, Graduate School of Agriculture, Kinki University, 3327-204 Nakamachi, Nara 631-8505, Japan. hiromi@nara.kindai.ac.jp
Yoshida
Gou
G
Baba
Yousuke
Y
Matsumura
Kenta
K
Wasada
Hiroshi
H
Murata
Jirou
J
Agawa
Mana
M
Itakura
Shuji
S
Enoki
Akio
A
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D005656:Fungal Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D006023:Glycoproteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D017665:Hydroxyl Radical / Q000378:metabolism*; D008954:Models, Biological; D008969:Molecular Sequence Data; D020075:Phanerochaete / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D005656:Fungal Proteins; D006023:Glycoproteins; D017665:Hydroxyl Radical