title: The FOX hunting system: an alternative gain-of-function gene hunting technique.
abstract: We have developed a novel gain-of-function system that we have named the FOX hunting system (Full-length cDNA Over-eXpressing gene hunting system). We used normalized full-length cDNA and introduced each cDNA into Arabidopsis by in planta transformation. About 10 000 independent full-length Arabidopsis cDNAs were expressed independently under the CaMV 35S promoter in Arabidopsis. Each transgenic Arabidopsis contained on average 2.6 cDNA clones and was monitored under various categories such as morphological changes, fertility and leaf color. We found 1487 possible morphological mutants from 15 547 transformants. When 115 pale green T(1) mutants were analyzed, 59 lines represented the mutant phenotypes in more than 50% of the T(2) progeny. Characterization of two leaf color mutants revealed the significance of this approach. We also document mutants from several categories and their corresponding full-length cDNAs.
authors.affiliation: Plant Functional Genomics Research Team, Plant Functional Genomics Research Group, Plant Science Center RIKEN Yokohama Institute, 1-7-22 Suehirocho, Tsurumiku, Yokohama, Kanagawa, 230-0045, Japan.
authors.forename: Takanari
authors.initials: T
authors.lastname: Ichikawa
authors.forename: Miki
authors.initials: M
authors.lastname: Nakazawa
authors.forename: Mika
authors.initials: M
authors.lastname: Kawashima
authors.forename: Haruko
authors.initials: H
authors.lastname: Iizumi
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Kuroda
authors.forename: Youichi
authors.initials: Y
authors.lastname: Kondou
authors.forename: Yumi
authors.initials: Y
authors.lastname: Tsuhara
authors.forename: Kumiko
authors.initials: K
authors.lastname: Suzuki
authors.forename: Akie
authors.initials: A
authors.lastname: Ishikawa
authors.forename: Motoaki
authors.initials: M
authors.lastname: Seki
authors.forename: Miki
authors.initials: M
authors.lastname: Fujita
authors.forename: Reiko
authors.initials: R
authors.lastname: Motohashi
authors.forename: Noriko
authors.initials: N
authors.lastname: Nagata
authors.forename: Tomoko
authors.initials: T
authors.lastname: Takagi
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shinozaki
authors.forename: Minami
authors.initials: M
authors.lastname: Matsui
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D018076:DNA, Complementary; D018744:DNA, Plant; D015723:Gene Library; D005821:Genetic Techniques; D018745:Genome, Plant; D016296:Mutagenesis; D009857:Oncogenes; D010641:Phenotype; D018515:Plant Leaves / Q000235:genetics; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012231:Rhizobium / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant