title: [Halophilous microbial groups in saline lake of Qinghai and the growth characteristics and anti-microbial and anti-tumor activities of F16].
abstract: A total of forty-five halophilous microorganisms were isolated from the sediment of saline lake in Qinghai Province, among which, filamentous fungus F16 showed the highest activity of anti-microorganism and anti-tumor. The ethyl acetate extract of F16 culture filtrate showed a strong cytotoxicity, and could inhibit the growth of four kinds of bacteria, especially Escherichia coli. When the concentration of the crude extract was 50 microg x ml(-1), the inhibition rate to liver cancer cell BEL7402 reached 76. 91%. The optimal temperature for F16 growth was 15 degrees C , and the increase of salt concentration in media would inhibit its growth. When the concentration of salt surpassed 15% , F16 could not survive. F16 grew well when the pH value ranged from 5 to 9.
authors.affiliation: College of Life Science and Biotechnology, Ningbo University, Ningbo 315211, China. yeyangfang@yahoo.com.cn
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Ye
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Yan
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Huang
authors.forename: Ye
authors.initials: Y
authors.lastname: Chen
authors.forename: Haimin
authors.initials: H
authors.lastname: Chen
authors.forename: Shihua
authors.initials: S
authors.lastname: Zhu
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification*; D000970:Antineoplastic Agents / Q000302:isolation & purification*; D001430:Bacteriocins / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology; D044822:Biodiversity; D002681:China; D005656:Fungal Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology; D005658:Fungi / Q000254:growth & development* / Q000302:isolation & purification / Q000502:physiology; D019015:Geologic Sediments / Q000382:microbiology; D006216:Halobacteriaceae / Q000254:growth & development* / Q000302:isolation & purification / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D001430:Bacteriocins; D005656:Fungal Proteins