Particle sizing with a fast polar nephelometer.
We reported previously the design of a polar nephelometer that uses a rotational confocal imaging setup to enable fast scanning of the scattering phase function within a field of view of 55 degrees . The full dynamic range of the detection system can be used by increasing the signal-to-noise ratio by means of averaging successive scans. The calibration of the angular response of the instrument is achieved by obtaining the transfer function of the optical detection system using Rayleigh scatterers. Accurate particle sizing of individual polystyrene spheres (ranging from 1.5 to 9 micro m in diameter) in aqueous suspension is achieved by maximizing a correlation coefficient between precalculated tables of Mie phase functions and data obtained from the polar nephelometer. Good correlation is achieved between experimental and theoretical data, proving the functioning of the instrument as a fast and convenient particle sizer.
Castagner
Jean-Luc
JL
Department of Biomedical Engineering, Boston University Photonics Center at Boston University, Boston, MA 02215, USA. jlcastagner@hotmail.com
Bigio
Irving J
IJ
D002138:Calibration; D018613:Microscopy, Confocal; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D055095:Optics and Photonics; D010316:Particle Size; D011137:Polystyrenes / Q000737:chemistry; D012542:Scattering, Radiation
D011137:Polystyrenes