title: Factors involved in binding of Lactobacillus plantarum Lp6 to rat small intestinal mucus.
abstract: OBJECTIVE
  To investigate the adhesion determinants of Lactobacillus plantarum Lp6, a dairy isolate.
  
  
  RESULTS
  Small intestinal mucus extracted from rats was used as a substrate for adhesion. Adhesion determinants were studied by physical, chemical and enzymatic pretreatments of the bacteria, and adhesion inhibition assay. The mannose-specific adhesins were explored by studying the effect of d-mannose on adhesion and the yeast-agglutinating ability of the bacteria. It was found that adhesion decreased after bacteria were treated with sodium metaperiodate, protease K, trypsin, lithium chloride and trichloroacetic acid. However, adhesion did not decrease after trypsin-treated bacteria were incubated with cell surface protein extract. Cell surface bound exopolysaccharides were found to inhibit the adhesion. D-mannose inhibited the adhesion in a dose-dependent manner. The bacteria could significantly agglutinate yeast and lost this ability after protease K treatment.
  
  
  CONCLUSIONS
  Adhesion was mainly mediated by the mannose specific adhesins, which might be proteins that reversibly bind to the cell surface components. Cell surface-bound exopolysaccharides were also involved in adhesion.
  
  
  CONCLUSIONS
  The mannose-specific adhesion of Lact. plantarum Lp6 to rat mucus might be important for competing with pathogens-binding sites in gut, which may be used to resist the colonization of the pathogens.
authors.affiliation: The Key Laboratory of Food Science and Safety, Ministry of Education, Southern Yangtze University, Wuxi, Jiangsu, China.
authors.forename: J
authors.initials: J
authors.lastname: Sun
authors.forename: G-W
authors.initials: GW
authors.lastname: Le
authors.forename: Y-H
authors.initials: YH
authors.lastname: Shi
authors.forename: G-W
authors.initials: GW
authors.lastname: Su
mesh_terms: D018829:Adhesins, Bacterial / Q000378:metabolism; D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D007413:Intestinal Mucosa / Q000378:metabolism / Q000382:microbiology*; D007421:Intestine, Small / Q000166:cytology*; D048191:Lactiplantibacillus plantarum / Q000166:cytology / Q000502:physiology*; D008358:Mannose / Q000378:metabolism*; D051381:Rats
chemical_list: D018829:Adhesins, Bacterial; D008358:Mannose