title: Site-specific labeling of DNA-protein conjugates by means of expressed protein ligation.
abstract: Site-specific bioconjugation of protein thioesters with a DNA oligonucleotide was achieved by Expressed Protein Ligation (EPL) and the new thiol group formed upon EPL in the conjugate was selectively coupled with small molecule labels using maleimide chemistry.
authors.affiliation: Universität Dortmund, Fachbereich Chemie, Biologisch-Chemische Mikrostrukturtechnik, Otto-Hahn Str. 6, D-44227 Dortmund, Germany.
authors.forename: Marina
authors.initials: M
authors.lastname: Lovrinovic
authors.forename: Ljiljana
authors.initials: L
authors.lastname: Fruk
authors.forename: Hendrik
authors.initials: H
authors.lastname: Schröder
authors.forename: Christof M
authors.initials: CM
authors.lastname: Niemeyer
mesh_terms: D002352:Carrier Proteins / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D007118:Immunoassay; D058619:Maltose-Binding Proteins; D015394:Molecular Structure
chemical_list: D002352:Carrier Proteins; D058619:Maltose-Binding Proteins; D004247:DNA