[Influence of bifidobacterium DNA on PKC and NF-kappaB in murine macrophages].
OBJECTIVE
To explore the influence of DNA of bifidobacteriua adolescence on PKC and NK-kappaB of murine macrophages.


METHODS
The fluorescent intensity of PKCalpha, PKCbetaI, PKCbetaII, PKCgamma, PKCepsilon and PKCzeta in murine peritoneal macrophages was detected by using laser confocal microscope. The density of NK-kappaB(+) macrophages was detected by immunocytochemical staining.


RESULTS
The average fluorescent intensity of PKCalpha and PKCbetaII produced by mouse peritoneal macrophages in bifidobacterium DNA injection group was markedly higher than that in control group(P<0.01). There was no statistically significant difference of average fluorescent intensity of PKCbetaI, PKCgamma, PKCepsilon and PKCzeta between the two groups (P>0.05). The density of NK-kappaB(+) macrophages in bifidobacterium DNA injection group was markedly higher than that in control group (P<0.01).


CONCLUSIONS
DNA of bifidobacteria adolescence could activate macrophages by promoting the activity of PKCalpha, PKCbetaII and NF-kappaB.
Wang
Li-sheng
LS
Department of Gastroenterology, the Second Clinical Medical Hospital, Jinan University, Shenzhen 518020, China. wangls168@163.com
Li
Ying-xue
YX
Zhu
Hui-ming
HM
Zhu
Zhong-sheng
ZS
Ma
Xiao-dong
XD
D000818:Animals; D001644:Bifidobacterium / Q000235:genetics*; D004269:DNA, Bacterial / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018613:Microscopy, Confocal; D016328:NF-kappa B / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D051571:Protein Kinase C-alpha / Q000378:metabolism; D051744:Protein Kinase C-epsilon / Q000378:metabolism
D004269:DNA, Bacterial; D016328:NF-kappa B; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha; D051744:Protein Kinase C-epsilon