title: Synthesis and serological interactions of H. pylori urease fragment 321-339 n-terminally immobilized on the cellulose.
abstract: Antigenic epitopes F8 (SIKEDVQF) and UB-33 (UreB fragment with residues 321-339: CHHLDKSIKEDVQFADSRI) in H. pylori urease that induces neutralizing antibody production were prepared on the cellulose plate from N- to C-terminus using CDMT as a coupling reagent. Reaction of both epitopes with sera of patients with medically confirmed atherosclerosis was studied. Strong, selective reactions of both peptides with some patients sera were observed.
authors.affiliation: Institute of Organic Chemistry, łódź University of Technology, Zeromskiego 116, 90-924 łódź, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Kolesińska
authors.forename: Sebastian
authors.initials: S
authors.lastname: Grabowski
authors.forename: Wiesław
authors.initials: W
authors.lastname: Kaca
authors.forename: Zbigniew J
authors.initials: ZJ
authors.lastname: Kamiński
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D015153:Blotting, Western; D002482:Cellulose; D004800:Enzymes, Immobilized; D000939:Epitopes / Q000276:immunology; D016480:Helicobacter pylori / Q000201:enzymology*; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000737:chemistry; D014510:Urease / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D004800:Enzymes, Immobilized; D000939:Epitopes; D010446:Peptide Fragments; D002482:Cellulose; D014510:Urease