Changes in estrogen receptors alpha and beta expression in the brain of mice exposed prenatally to bisphenol A.
The expression of ERs alpha and beta and serotonergic neurons were evaluated in the brains of mice prenatally exposed to Bisphenol A, a known endocrine disrupting chemical (EDc). Bisphenol A was administered orally at a dose of 2ng/g body weight on gestinational days 11-17 to pregnant ICR mice. Newborn male offspring (Bis-A mice) were evaluated for the immunoreactivity of ERs alpha and beta, serotonin, and serotonin transporter positive cells in the dorsal raphe nucleus (DRN). The serum testosterone level was also evaluated. In the Bis-A mice, the expression of ERs alpha and beta at 5 and 13 weeks was increased compared with the controls (P<0.04), but this difference disappeared by the 9th week. The serotonin, serotonin transporter, and testosterone level differences between two groups did not reach significance. Exposure to bisphenol A may have changed the expression of ERs in the brain, but did not directly affect serotonin neurons in the DRN.
Kawai
Keisuke
K
Department of Psychosomatic Medicine, Graduate School of Medical Sciences, Kyushu University, Japan. kawai@cephal.med.kyushu-u.ac.jp <kawai@cephal.med.kyushu-u.ac.jp>
Murakami
Shuji
S
Senba
Emiko
E
Yamanaka
Takeharu
T
Fujiwara
Yuya
Y
Arimura
Chikako
C
Nozaki
Takehiro
T
Takii
Masato
M
Kubo
Chiharu
C
D000818:Animals; D001559:Benzhydryl Compounds; D001921:Brain; D047628:Estrogen Receptor alpha / Q000187:drug effects* / Q000378:metabolism; D047629:Estrogen Receptor beta / Q000187:drug effects* / Q000378:metabolism; D004968:Estrogens, Non-Steroidal / Q000494:pharmacology / Q000633:toxicity*; D005260:Female; D005333:Fetus / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010636:Phenols / Q000494:pharmacology / Q000633:toxicity*; D011247:Pregnancy; D012701:Serotonin / Q000378:metabolism; D050486:Serotonin Plasma Membrane Transport Proteins / Q000187:drug effects / Q000378:metabolism; D013739:Testosterone / Q000097:blood; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004968:Estrogens, Non-Steroidal; D010636:Phenols; D012701:Serotonin; D050486:Serotonin Plasma Membrane Transport Proteins; D013739:Testosterone; C006780:bisphenol A; D000098826:Bisphenol A Compounds