Induction of dendritic cell-mediated immune responses against canine malignant melanoma cells.
To establish the basis for the use of dendritic cells (DC) in the treatment of canine melanoma, dogs were vaccinated using autologous DC pulsed with canine melanoma CMM2 cell lysate in the presence of keyhole limpet haemocyanin (KLH) in vitro (CMM2-KLH-DC), and the induction of immune responses against CMM2 cells in vivo was examined using the delayed-type hypersensitivity (DTH) skin test. The DTH responses against CMM2 cells and KLH were observed in dogs vaccinated with CMM2-KLH-DC, while the responses against KLH but not CMM2 cells were detected with DC pulsed with KLH alone (KLH-DC). Recruitment of CD8 and CD4 T cells was detected in the positively responding sites, suggested that vaccination with CMM2-KLH-DC efficiently elicits T cell-mediated immunity against CMM-2 cells in vivo. These findings demonstrate the potential utility of DC-based tumour vaccination in the treatment of canine malignant melanoma.
Tamura
Kyoichi
K
Department of Veterinary Clinical Pathology, Nippon Veterinary and Life Science University, Tokyo 180-8602, Japan.
Yamada
Misato
M
Isotani
Mayu
M
Arai
Hiroyoshi
H
Yagihara
Hiroko
H
Ono
Kenichiro
K
Washizu
Tsukimi
T
Bonkobara
Makoto
M
D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology; D019496:Cancer Vaccines / Q000008:administration & dosage; D003713:Dendritic Cells / Q000276:immunology*; D004283:Dog Diseases / Q000276:immunology*; D004285:Dogs; D008545:Melanoma / Q000276:immunology / Q000662:veterinary*; D012878:Skin Neoplasms / Q000276:immunology / Q000662:veterinary*; D014611:Vaccination / Q000662:veterinary
D000951:Antigens, Neoplasm; D019496:Cancer Vaccines