Solution NMR of large molecules and assemblies.
Solution NMR spectroscopy represents a powerful tool for examining the structure and function of biological macromolecules. The advent of multidimensional (2D-4D) NMR, together with the widespread use of uniform isotopic labeling of proteins and RNA with the NMR-active isotopes, 15N and 13C, opened the door to detailed analyses of macromolecular structure, dynamics, and interactions of smaller macromolecules (< approximately 25 kDa). Over the past 10 years, advances in NMR and isotope labeling methods have expanded the range of NMR-tractable targets by at least an order of magnitude. Here we briefly describe the methodological advances that allow NMR spectroscopy of large macromolecules and their complexes and provide a perspective on the wide range of applications of NMR to biochemical problems.
Foster
Mark P
MP
Department of Biochemistry, The Ohio State University, 484 West 12th Avenue, Columbus, Ohio 43210, USA. foster.281@osu.edu
McElroy
Craig A
CA
Amero
Carlos D
CD
D001665:Binding Sites; D046911:Macromolecular Substances; D008958:Models, Molecular; D046912:Multiprotein Complexes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D009690:Nucleic Acid Conformation; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D012996:Solutions; D013816:Thermodynamics
D046911:Macromolecular Substances; D046912:Multiprotein Complexes; D011506:Proteins; D012996:Solutions; D012313:RNA