Technical note: detection of chicken, turkey, duck, and goose tissues in feedstuffs using species-specific polymerase chain reaction.
PCR method was applied for the qualitative identification of chicken (Gallus gallus), turkey (Meleagris gallipavo), duck (Anas platyrhynchos x Cairina muschata), and goose (Anser anser) tissues in feed-stuffs, on an individual basis. The assay uses oligonucleotide primers that are specific for each avian species, targeting the 12S rRNA mitochondrial gene. The primers designed generated amplicons of 95, 122, 64, and 98 bp length for chicken, turkey, duck, and goose, respectively. The specificity of the primers was tested against 29 animal species including mammals, birds, and fish, as well as 8 plant species. Analysis of experimental feedstuffs demonstrated the detection of each target species in the range of 0.1 to 100%. The performance of this method was not affected by prolonged heat-treatment (up to 133 degrees C for 20 min at 300 kPa), and consequently, it could be very useful for the accurate identification of tissues from these 4 avian species in products submitted to denaturing technologies, for which other methods cannot be applied.
Martín
I
I
Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain.
García
T
T
Fajardo
V
V
López-Calleja
I
I
Rojas
M
M
Pavón
M A
MA
Hernández
P E
PE
González
I
I
Martín
R
R
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D004247:DNA / Q000032:analysis; D017931:DNA Primers / Q000737:chemistry; D005504:Food Analysis / Q000379:methods*; D006358:Hot Temperature; D009245:NADH Dehydrogenase / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D011200:Poultry / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics; D012680:Sensitivity and Specificity; D013045:Species Specificity
D017931:DNA Primers; D012335:RNA, Ribosomal; C075526:RNA, ribosomal, 12S; D004247:DNA; D009245:NADH Dehydrogenase