title: Evaluation of the surface free energy on root canal dentine walls treated with chelating agents and NaOCl.
abstract: OBJECTIVE
  To evaluate ex vivo the effects of combined and single use of EDTA, RC-Prep and NaOCl on the surface free energy of canal wall dentine using the captive bubble technique.
  
  
  METHODS
  Eighteen extracted human pre-molar teeth were sectioned at the crown and the apical third, the remaining mid-root portion were bisected longitudinally. Thereafter, the root halves were embedded in resin blocks that exposed the dentine surface of the canal wall. The specimens were randomly assigned to six experimental groups (n = 6) after polishing. The root dentine surfaces of the first two groups were treated with 17% EDTA or RC-Prep followed by 2.5% NaOCl irrigation. Groups 3, 4 and 5 were treated with either 17% EDTA, RC-Prep or 2.5% NaOCl alone. Control specimens were irrigated with saline solution. The surface free energies of experimental groups were calculated by measuring air and octane contact angles on the canal wall dentine. Statistical analysis was performed using the Mann-Whitney U and Bonferroni post-tests at P = 0.05.
  
  
  RESULTS
  Compared with the control group; combined and single use of EDTA, RC-Prep and NaOCl irrigation significantly decreased the surface free energy of canal wall dentine surfaces (P < 0.05). Among all groups tested, the use of NaOCl as a final flush following RC-Prep treatment yielded increased wettability. Nevertheless, this value remained lower than that of the control group.
  
  
  CONCLUSIONS
  Use of chelating agents alone or in combination with NaOCl decreased the wettability of root canal wall dentine.
authors.affiliation: Department of Endodontics, Faculty of Dentistry, Hacettepe University, Ankara, Turkey. hdogan67@hotmail.com
authors.forename: H
authors.initials: H
authors.lastname: Dogan Buzoglu
authors.forename: S
authors.initials: S
authors.lastname: Calt
authors.forename: M
authors.initials: M
authors.lastname: Gümüsderelioglu
mesh_terms: D001641:Bicuspid; D002614:Chelating Agents / Q000008:administration & dosage*; D003786:Dental Pulp Cavity; D003804:Dentin; D004492:Edetic Acid / Q000008:administration & dosage; D006801:Humans; D010545:Peroxides / Q000008:administration & dosage; D012388:Root Canal Irrigants / Q000008:administration & dosage*; D016369:Smear Layer; D012973:Sodium Hypochlorite / Q000008:administration & dosage; D018709:Statistics, Nonparametric; D013499:Surface Properties; D014508:Urea / Q000008:administration & dosage; D014885:Waxes; D017155:Wettability
chemical_list: D002614:Chelating Agents; D010545:Peroxides; D012388:Root Canal Irrigants; D014885:Waxes; C025081:RC-Prep; D014508:Urea; D004492:Edetic Acid; D012973:Sodium Hypochlorite