The expression analysis of ICOS-L on activated T cells and immature dendritic cells as well as malignant B cells and Grave's-disease-derived thyroid tissues by two novel mAbs against human ICOS-L.
ICOS-L, a newly identified member of B7 superfamily, plays an important role in immune responses. In this article, we report on two novel mouse anti-human ICOS-L monoclonal antibodies (mAbs) named as 11C4 and 12B11, whose specificities were verified by methods of flow cytometry, western blotting, and epitope competition assay. The two mAbs bound to distinct ICOS-L epitopes on B cells. Interestingly, mAb 11C4 could well recognize ICOS-L molecule on activated T cells and Jurkat cell lines, which is different from commercial anti-ICOS-L mAb (clone number MIH12) and the other mAb 12B11. In addition, we found that the expression of ICOS-L molecule was only detected on the surface of immature monocyte-derived dendritic cells (Mo-DCs) and was sharply decreased after induction of mature Mo-DCs activated by tumor necrosis factor-alpha or CD40. Furthermore, we showed that 11C4 could effectively suppress the maturation of Mo-DCs in vitro as evidenced by the low expression of CD80, CD86, CD83, and human leukocyte antigen-DR, which suggested that ICOS-L may be involved in the maturation of Mo-DCs. Using immunohistochemistry staining with mAb 11C4, the expression of ICOS-L was found in B lymphoma tissues and thyroid tissues from the Grave's disease but not in thyroid adenoma and normal thyroid tissues.
Wang
F
F
Medical Biotechnology Institute, and Stem Cell Research Laboratory of Jiangsu Province, Soochow University, Suzhou, People's Republic of China.
Zhu
W
W
Liu
T
T
Sun
Z
Z
Ju
S
S
Ju
S
S
Yu
G
G
Xie
W
W
Deng
Z
Z
Lu
B
B
Zhang
X
X
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology*; D015703:Antigens, CD; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism*; D006111:Graves Disease / Q000276:immunology / Q000378:metabolism*; D018922:HL-60 Cells; D006801:Humans; D060945:Inducible T-Cell Co-Stimulator Ligand; D019169:Jurkat Cells; D016393:Lymphoma, B-Cell / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D011506:Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D013961:Thyroid Gland / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; C498514:ICOSLG protein, human; D060945:Inducible T-Cell Co-Stimulator Ligand; D011506:Proteins