Oxylipin-coated hat-shaped ascospores of Ascoidea corymbosa.
We previously implicated 3-hydroxy oxylipins and ascospore structure in ascospore release from enclosed asci. Using confocal laser scanning microscopy on cells stained with fluorescein-coupled, 3-hydroxy oxylipin-specific antibodies, we found that oxylipins are specifically associated with ascospores and not the vegetative cells or ascus wall of Ascoidea corymbosa. Using gas chromatography--mass spectrometry the oxylipin 3-hydroxy 17:0 could be identified. Here, we visualize for the first time the forced release of oxylipin-coated, hat-shaped ascospores from terminally torn asci, probably through turgor pressure. We suggest that oxylipin-coated, razor-sharp, hat-shaped ascospore brims may play a role in rupturing the ascus to affect release.
Ncango
Desmond M
DM
UNESCO MIRCEN: Industrial Biotchnology, Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, Bloemfontein, South Africa.
Pohl
Carolina H
CH
Sebolai
Olihile M
OM
Botes
Piet J
PJ
Strauss
Catharina J
CJ
Joseph
Manjusha
M
Van Wyk
Pieter W J
PW
Nigam
Santosh
S
Kock
Johan L F
JL
D002473:Cell Wall / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D008055:Lipids / Q000737:chemistry / Q000302:isolation & purification*; D018613:Microscopy, Confocal; D004718:Saccharomycetales / Q000737:chemistry / Q000166:cytology*; D013172:Spores, Fungal / Q000737:chemistry / Q000166:cytology*
D008055:Lipids