title: Positive and negative regulation of Tetrahymena telomerase holoenzyme.
abstract: Telomerase replenishes the telomeric repeats that cap eukaryotic chromosome ends. To perform DNA synthesis, the active site of telomerase reverse transcriptase (TERT) copies a template within the integral telomerase RNA (TER). In vivo, TERT and TER and additional subunits form a telomerase holoenzyme capable of telomere elongation. We previously purified epitope-tagged Tetrahymena thermophila TERT and characterized two of the associated proteins. Here we characterize the remaining two proteins that were enriched by TERT purification. The primary sequence of the p75 polypeptide lacks evident homology with other proteins, whereas the p20 polypeptide is the Tetrahymena ortholog of a conserved multifunctional protein, Skp1. Genetic depletion of p75 induced telomere shortening without affecting the accumulation of TER or TERT, suggesting that p75 promotes telomerase function at the telomere. Affinity purification of p75 coenriched telomerase activity and each other known telomerase holoenzyme protein. On the other hand, genetic depletion of Skp1p induced telomere elongation, suggesting that this protein plays a negative regulatory role in the maintenance of telomere length homeostasis. Affinity purification of Skp1p did not detectably enrich active telomerase but did copurify ubiquitin ligase machinery. These studies reveal additional complexity in the positive and negative regulation of Tetrahymena telomerase function.
authors.affiliation: Department of Molecular and Cell Biology, University of California at Berkeley, CA 94720-3204, USA.
authors.forename: Keren L
authors.initials: KL
authors.lastname: Witkin
authors.forename: Ramadevi
authors.initials: R
authors.lastname: Prathapam
authors.forename: Kathleen
authors.initials: K
authors.lastname: Collins
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D017353:Gene Deletion; D015971:Gene Expression Regulation, Enzymologic; D020035:Holoenzymes / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D010641:Phenotype; D011485:Protein Binding; D044786:S-Phase Kinase-Associated Proteins / Q000378:metabolism; D019098:Telomerase / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D013768:Tetrahymena / Q000201:enzymology* / Q000235:genetics
chemical_list: D000906:Antibodies; D018076:DNA, Complementary; D020035:Holoenzymes; D010455:Peptides; D044786:S-Phase Kinase-Associated Proteins; D019098:Telomerase