Physiological proteomics of the uncultured endosymbiont of Riftia pachyptila.
The bacterial endosymbiont of the deep-sea tube worm Riftia pachyptila has never been successfully cultivated outside its host. In the absence of cultivation data, we have taken a proteomic approach based on the metagenome sequence to study the metabolism of this peculiar microorganism in detail. As one result, we found that three major sulfide oxidation proteins constitute approximately 12% of the total cytosolic proteome, which highlights the essential role of these enzymes for the symbiont's energy metabolism. Unexpectedly, the symbiont uses the reductive tricarboxylic acid cycle in addition to the previously identified Calvin cycle for CO2 fixation.
Markert
Stephanie
S
Institute of Marine Biotechnology, Walther-Rathenau-Strasse 49, D-17489 Greifswald, Germany.
Arndt
Cordelia
C
Felbeck
Horst
H
Becher
Dörte
D
Sievert
Stefan M
SM
Hügler
Michael
M
Albrecht
Dirk
D
Robidart
Julie
J
Bench
Shellie
S
Feldman
Robert A
RA
Hecker
Michael
M
Schweder
Thomas
T
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D052837:Chemoautotrophic Growth; D002952:Citric Acid Cycle; D003600:Cytosol / Q000378:metabolism; D017753:Ecosystem; D004734:Energy Metabolism; D020563:Gammaproteobacteria / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D016680:Genome, Bacterial; D006862:Hydrogen Sulfide / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D053858:Metabolic Networks and Pathways; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010140:Pacific Ocean; D011077:Polychaeta / Q000382:microbiology*; D020543:Proteome; D040901:Proteomics; D013455:Sulfur / Q000378:metabolism; D013559:Symbiosis; D013696:Temperature
D001426:Bacterial Proteins; D020543:Proteome; D002245:Carbon Dioxide; D013455:Sulfur; D002244:Carbon; D006862:Hydrogen Sulfide