Synergistic effects of pravastatin and pioglitazone in renal tubular epithelial cells induced by transforming growth factor-beta1.
Recent studies suggest that treatment with PPAR-gamma agonists and statins have beneficial effects on renal disease. However, the combined effects of PPAR-gamma agonists and statins in human renal epithelial cells are unknown. Our present study revealed that there were synergistic effects of pravastatin and pioglitazone in the expression of alpha-smooth muscle actin (alpha-SMA), connective tissue growth factor (CTGF), fibronectin (FN), plasminogen activator inhibitor-1 (PAI-1) and collagen 1 in human renal proximal tubular epithelial cells induced by transforming growth factor-beta 1 (TGF-beta1). The beneficial effects of combined therapy against renal tubular epithelial cell injury are attributed, at least in part, to the inhibition of transdifferentiation, extracellular matrix deposition and cytokine production.
Wei
Jia-li
JL
Department of Cardio, Renal and Cerebrovascular Medicine, Faculty of Medicine, Kagawa University, Kagawa, Japan. jlwei525@yahoo.com.cn
Ma
Chun-yang
CY
Zhang
Yang-De
YD
Li
Yiming
Y
D000199:Actins / Q000235:genetics; D002460:Cell Line; D024042:Collagen Type I / Q000235:genetics; D055513:Connective Tissue Growth Factor; D005353:Fibronectins / Q000235:genetics; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics; D007687:Kidney Tubules, Proximal / Q000166:cytology* / Q000187:drug effects; D032389:Myocytes, Smooth Muscle / Q000166:cytology; D047495:PPAR gamma / Q000819:agonists; D000077205:Pioglitazone; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics; D017035:Pravastatin / Q000494:pharmacology*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D045162:Thiazolidinediones / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*; D019459:Urothelium / Q000166:cytology* / Q000187:drug effects
D000199:Actins; C526317:CCN2 protein, human; D024042:Collagen Type I; D005353:Fibronectins; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D047495:PPAR gamma; D017395:Plasminogen Activator Inhibitor 1; D045162:Thiazolidinediones; D053773:Transforming Growth Factor beta1; D055513:Connective Tissue Growth Factor; D012313:RNA; D017035:Pravastatin; D000077205:Pioglitazone