Activation effects of Allium tuberosum Rottl. on choline acetyltransferase.
An ethanolic extract of Allium tuberosum Rottl. demonstrated potent choline acetytrasferase (ChAT) activating activity (45%) among 30 screened Korean edible plants. The ChAT activator of A. tuberoum Rottl. was purified and identified as ferulic acid [4-hydroxy-3-methoxycinnamic acid] via silica-gel open-column chromatography, HPLC, EI-MS, and 1H/13C-NMR. The ferulic acid from A. tuberoum Rottl. exhibited ChAT activity in a dose-dependent manner and showed notoxicity in a cell viability assay (MTT assay). We suggest that the ferulic acid from A. tuberoum Rottl. was the strong ChAT activator.
Kim
Mi Jeong
MJ
Graduate School of Life Sciences and Biotechnology, Korea University, South Korea.
Choi
Soo Jung
SJ
Kim
Hye Kyung
HK
Kim
Chang-Ju
CJ
Hong
Bumshik
B
Kim
Young Jun
YJ
Shin
Dong-Hoon
DH
D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D027782:Chive / Q000737:chemistry*; D002795:Choline O-Acetyltransferase / Q000378:metabolism*; D003373:Coumaric Acids / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology
D003373:Coumaric Acids; D010936:Plant Extracts; C004999:ferulic acid; D002795:Choline O-Acetyltransferase