title: Chiral separation of three agrochemical toxins enantiomers by high-performance liquid chromatography on a vancomycin crystalline degradation products-chiral stationary phase.
abstract: This work aims to evaluate for the enantiomeric separations of three agrochemical toxins: haloxyfop-methyl, fenoxaprop-p-ethyl and indoxacarb on crystalline degradation products-chiral stationary phase (CDP-CSP) of high-performance liquid chromatography (HPLC) under normal and polar organic phases. In the normal phase, the mobile phase was n-hexane with alcohols including methanol and isopropanol as polar modifiers. In the polar organic phase mode, the mobile phase was methanol with different percentages of triethylammunium acetate. The influence of flow rate (0.3-0.9 mL/min), analyte concentration and silica gel particle sizes (10, 15 and 30 microm) was investigated. This new chiral stationary phase showed excellent stereoselectivity for the two enantiomers of haloxyfop-methyl and fenoxaprop-p-ethyl and chiral recognition for indoxacarb under normal-phase mode. However, under polar organic phase, only indoxacarb was separated (alpha < 1.5). The chromatographic results were compared with commercial chiral columns.
authors.affiliation: Chemistry and Chemical Engineering Research Center, 657, Soheil St, Kordestan Freeway, PO Box 14335-186, Tehran, Iran.
authors.forename: Mohammad Majid
authors.initials: MM
authors.lastname: Mojtahedi
authors.forename: Sohila
authors.initials: S
authors.lastname: Chalavi
authors.forename: Alireza
authors.initials: A
authors.lastname: Ghassempour
authors.forename: Kourosh
authors.initials: K
authors.lastname: Tabar-Heydar
authors.forename: Seyed Javad Ghotb
authors.initials: SJ
authors.lastname: Sharif
authors.forename: Maryam
authors.initials: M
authors.lastname: Malekzadeh
authors.forename: Hassan Y
authors.initials: HY
authors.lastname: Aboul-Enein
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D016573:Agrochemicals / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000432:Methanol / Q000737:chemistry; D015394:Molecular Structure; D010078:Oxazines; D010080:Oxazoles; D010575:Pesticides / Q000302:isolation & purification*; D011422:Propionates; D011725:Pyridines; D012822:Silicon Dioxide / Q000737:chemistry; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013237:Stereoisomerism; D013696:Temperature; D014640:Vancomycin / Q000737:chemistry*
chemical_list: D000097:Acetonitriles; D016573:Agrochemicals; D010078:Oxazines; D010080:Oxazoles; D010575:Pesticides; D011422:Propionates; D011725:Pyridines; D012997:Solvents; C054176:haloxyfop; C401104:indoxacarb; D014640:Vancomycin; D012822:Silicon Dioxide; C416661:fenoxaprop ethyl; D000432:Methanol