title: Analysis of magnolol and honokiol in biological fluids by capillary zone electrophoresis.
abstract: Capillary zone electrophoresis (CZE) method was used for analysis of magnolol and honokiol. Under the optimized condition, CZE with UV absorption detection provided that the limit of detection was at microM level. To enhance detection sensitivity of magnolol and honokiol, CZE separation system was coupled with a laser-induced fluorescence (LIF) detector for the first time. The limits of detection of magnolol and honokiol were 12 nM (3.20 ng ml(-1)) and 18 nM (4.79 ng ml(-1)), respectively, showing that the CZE-LIF system provides greater than 100-fold sensitivity improvements than does the CZE-UV system. The developed method was applied to analyze magnolol and honokiol in spiked human plasma samples, microsome incubation samples as a preliminary demonstration of its potential in pharmacokinetic studies.
authors.affiliation: School of Pharmacy, College of Medicine, National Taiwan University, Taipei, Taiwan.
authors.forename: Chiu-Ling
authors.initials: CL
authors.lastname: Chen
authors.forename: Po-Ling
authors.initials: PL
authors.lastname: Chang
authors.forename: Shoei-Sheng
authors.initials: SS
authors.lastname: Lee
authors.forename: Fu-Chuo
authors.initials: FC
authors.lastname: Peng
authors.forename: Ching-Hua
authors.initials: CH
authors.lastname: Kuo
authors.forename: Huan-Tsung
authors.initials: HT
authors.lastname: Chang
mesh_terms: D000818:Animals; D001713:Biphenyl Compounds / Q000032:analysis* / Q000097:blood; D019075:Electrophoresis, Capillary / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D017705:Lignans / Q000032:analysis* / Q000097:blood; D008862:Microsomes, Liver / Q000737:chemistry; D051381:Rats; D012015:Reference Standards; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D000498:Allyl Compounds; D010636:Phenols
chemical_list: D001713:Biphenyl Compounds; D017705:Lignans; C005498:magnolol; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols