1-O-alkylglycerols reduce the stimulating effects of bFGF on endothelial cell proliferation in vitro.
1-O-alkylglycerols (alkyl-Gro) are natural etherlipids with multiple biological activities. We previously demonstrated that alkyl-Gro reduce endothelial permeability. Here we showed that alkyl-Gro reduced the basic Fibroblast Growth Factor (bFGF)-stimulated endothelial cell proliferation in a concentration-dependent manner. The effects of 0.5 and 5 ng/ml bFGF on growth were completely suppressed after 72 h-treatment by 50 microM alkyl-Gro. Since bFGF greatly increased (+56%+/-15) the production of 1-O-alkyl-2-acyl-sn-glycerophosphate in alkyl-Gro-treated endothelial cells, our data suggest that the observed effects of alkyl-Gro could be mediated through PLD activation. Inhibition of bFGF-stimulated endothelial proliferation could support anti-angiogenic activity of alkyl-Gro.
Pédrono
Frédérique
F
Laboratoire de Pharmacologie Moléculaire, Faculté de Pharmacie, Université de Rennes I, Rennes, France.
Saïag
Bernard
B
Moulinoux
Jacques-Philippe
JP
Legrand
Alain B
AB
D020533:Angiogenesis Inhibitors; D000818:Animals; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects*; D004730:Endothelium, Vascular; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005990:Glycerol / Q000031:analogs & derivatives* / Q000494:pharmacology; D005995:Glyceryl Ethers / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D020533:Angiogenesis Inhibitors; D005995:Glyceryl Ethers; D016222:Fibroblast Growth Factor 2; D005990:Glycerol