Alternative donor substrates for inverting and retaining glycosyltransferases.
By the use of glycosyl donors containing aromatic leaving groups linked with opposite anomeric configurations compared to those of the natural donor substrates, an inverting (Cst II) and a retaining (LgtC) glycosyltransferase were found to catalyse glycosylation reactions of natural acceptor substrates in the presence of the corresponding nucleotide.
Lairson
Luke L
LL
Department of Chemistry, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z1.
Wakarchuk
Warren W
WW
Withers
Stephen G
SG
D002236:Carbohydrate Conformation; D002384:Catalysis; D016695:Glycosyltransferases / Q000378:metabolism*; D013379:Substrate Specificity
D016695:Glycosyltransferases