title: Prior facial motor neuron injury elicits endogenous T cell memory: relation to neuroregeneration.
abstract: We tested the hypotheses that prior injury to the facial motor nucleus (FMN) would elicit a more robust T cell response in the opposite FMN when the contralateral facial nerve was injured later in life, and that this would result in improved neuroregeneration. Measures of T cell, neuronal and microglial status were compared in sensitized mice (right facial nerve transection followed by contralateral facial nerve transection 9.5 weeks later) and naïve mice (sham surgery of the right facial nerve followed by contralateral facial nerve transection 9.5 weeks later) following axotomy of the contralateral facial nerve. At day 14 post-axotomy, sensitized mice exhibited nearly a two-fold increase in T cells in the FMN compared to naïve mice. There were no differences between the groups in levels of dead neurons and NeuN expression by surviving motor neurons at day 14, or motor neuron survival and cell area at day 49 post-axotomy. Measures of microglial responsiveness did not differ between the groups. Further study is needed to delineate the role of endogenous T cell memory in neuronal injury and regeneration.
authors.affiliation: Department of Neuroscience, McKnight Brain Institute, University of Florida, Gainesville, FL 32610, USA.
authors.forename: Grace K
authors.initials: GK
authors.lastname: Ha
authors.forename: Zhi
authors.initials: Z
authors.lastname: Huang
authors.forename: John M
authors.initials: JM
authors.lastname: Petitto
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D039481:CD11b Antigen / Q000378:metabolism; D015704:CD4 Antigens / Q000378:metabolism; D020220:Facial Nerve Injuries / Q000473:pathology / Q000503:physiopathology*; D005260:Female; D007839:Functional Laterality / Q000502:physiology; D007150:Immunohistochemistry / Q000379:methods; D008213:Lymphocyte Activation / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009416:Nerve Regeneration / Q000502:physiology*; D009687:Nuclear Proteins / Q000378:metabolism; D011149:Pons / Q000473:pathology*; D013601:T-Lymphocytes / Q000378:metabolism / Q000473:pathology*; D015534:Trans-Activators / Q000378:metabolism
chemical_list: D039481:CD11b Antigen; D015704:CD4 Antigens; C083728:MHC class II transactivator protein; D009687:Nuclear Proteins; D015534:Trans-Activators