title: Effects of a new antibacterial adhesive on the repair capacity of the pulp-dentine complex in infected teeth.
abstract: OBJECTIVE
  To evaluate the effects of a self-etching/priming adhesive system, containing the antibacterial monomer 12-methacryloyloxy-dodecylpyridinium bromide (MDPB), on the repair capacity of the pulp-dentine complex in infected cavities in dog's teeth.
  
  
  METHODS
  Class V cavities with a residual dentine thickness ranging from 0.3-0.8 mm were prepared on the buccal surface of permanent teeth in four dogs. Pulpal exposures were performed in half of the cavities. Millipore filters that had been incubated for 3 h in a 10(5) milky suspension of a-streptococci were placed in the cavities, which were then filled temporarily. After 24 h, the filters were removed and both the exposed and non-exposed cavities were washed with sterile saline and assigned to four groups which were treated with either the experimental antibacterial adhesive system, or Clearfil SE bond, Dycal and Teflon discs. Stereotype connective tissue reactions (inflammatory cell response and/or tissue necrosis) and pulp-specific reparative tissue responses (reduction of odontoblasts and tertiary dentine formation) were assessed at post-operative periods of 4 and 8 weeks.
  
  
  RESULTS
  Neither severe inflammation nor tissue necrosis was observed, either in the dentinal cavities or pulpal exposures treated with the self-etch adhesive containing MDPB. Rates of tertiary dentine formation in infected dentinal cavities treated with this system were comparable with those observed after dentine treatment with the Ca(OH)2-based material. Dentinal bridging was not seen in pulpal exposures treated with the experimental adhesive.
  
  
  CONCLUSIONS
  The new antibacterial adhesive system maintained pulp vitality and primary odontoblastic function in infected nonexposed and exposed cavities but interfered with reparative dentine formation in infected pulpal exposures.
authors.affiliation: Department of Endodontology, School of Dentistry, Aristotle University of Thessaloniki, Thessaloniki, Greece. dtziaf@dent.auth.gr
authors.forename: D
authors.initials: D
authors.lastname: Tziafas
authors.forename: E
authors.initials: E
authors.lastname: Koliniotou-Koumpia
authors.forename: C
authors.initials: C
authors.lastname: Tziafa
authors.forename: S
authors.initials: S
authors.lastname: Papadimitriou
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology* / Q000627:therapeutic use; D002126:Calcium Hydroxide / Q000494:pharmacology / Q000627:therapeutic use; D003782:Dental Pulp / Q000187:drug effects* / Q000382:microbiology; D003785:Dental Pulp Capping / Q000379:methods*; D003789:Dental Pulp Exposure / Q000382:microbiology / Q000628:therapy; D003793:Dental Restoration, Permanent / Q000379:methods*; D003804:Dentin / Q000187:drug effects / Q000382:microbiology; D003809:Dentin, Secondary / Q000378:metabolism; D017220:Dentin-Bonding Agents / Q000494:pharmacology* / Q000627:therapeutic use; D004285:Dogs; D008903:Minerals / Q000494:pharmacology / Q000627:therapeutic use; D011726:Pyridinium Compounds / Q000494:pharmacology* / Q000627:therapeutic use; D012387:Root Canal Filling Materials / Q000494:pharmacology / Q000627:therapeutic use; D013290:Streptococcal Infections / Q000188:drug therapy; D034363:Viridans Streptococci / Q000187:drug effects; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000891:Anti-Infective Agents, Local; D017220:Dentin-Bonding Agents; D008903:Minerals; D011726:Pyridinium Compounds; D012387:Root Canal Filling Materials; C089392:methacryloyloxydodecylpyridinium bromide; C025702:Dycal; D002126:Calcium Hydroxide