[Halophilous microbial groups in saline lake of Qinghai and the growth characteristics and anti-microbial and anti-tumor activities of F16].
A total of forty-five halophilous microorganisms were isolated from the sediment of saline lake in Qinghai Province, among which, filamentous fungus F16 showed the highest activity of anti-microorganism and anti-tumor. The ethyl acetate extract of F16 culture filtrate showed a strong cytotoxicity, and could inhibit the growth of four kinds of bacteria, especially Escherichia coli. When the concentration of the crude extract was 50 microg x ml(-1), the inhibition rate to liver cancer cell BEL7402 reached 76. 91%. The optimal temperature for F16 growth was 15 degrees C , and the increase of salt concentration in media would inhibit its growth. When the concentration of salt surpassed 15% , F16 could not survive. F16 grew well when the pH value ranged from 5 to 9.
Ye
Yanfang
Y
College of Life Science and Biotechnology, Ningbo University, Ningbo 315211, China. yeyangfang@yahoo.com.cn
Yan
Xiaojun
X
Huang
Xiaochun
X
Chen
Ye
Y
Chen
Haimin
H
Zhu
Shihua
S
D000900:Anti-Bacterial Agents / Q000302:isolation & purification*; D000970:Antineoplastic Agents / Q000302:isolation & purification*; D001430:Bacteriocins / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology; D044822:Biodiversity; D002681:China; D005656:Fungal Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology; D005658:Fungi / Q000254:growth & development* / Q000302:isolation & purification / Q000502:physiology; D019015:Geologic Sediments / Q000382:microbiology; D006216:Halobacteriaceae / Q000254:growth & development* / Q000302:isolation & purification / Q000502:physiology
D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D001430:Bacteriocins; D005656:Fungal Proteins