Comparison of two polymer-based immunohistochemical detection systems: ENVISION+ and ImmPRESS.
The non-specific background reaction produced in avidin-biotin-based immunohistochemistry, particularly after harsh antigen retrieval procedures, has promoted the use of non-avidin-biotin systems, yet there are few reports comparing the performance of non-avidin-biotin, polymer-based methods. In this study we compare two of these methods, ENVISION+trade mark and ImmPRESS, in animal tissues. We examined the immunoreactivity of 18 antigens in formalin-fixed, paraffin-embedded tissues. Antigens were located in the cytoplasmic membrane (CD11d, CD18 and CD79a), cytoplasm (calretinin, COX-1, COX-2, Glut-1, HepPar 1, KIT, Melan A, tryptase and uroplakin III) or nucleus (MUM-1, PGP 9.5 and thyroid transcription factor 1). We also evaluated three infectious agents (Aspergillus, calicivirus and West Nile virus). The staining with ENVISION+ or ImmPRESS was performed simultaneously for each antigen. The intensity of the reaction and background staining were scored. ImmPRESS yielded similar or higher reaction intensity than ENVISION+trade mark in 16/18 antigens. ImmPRESS produced abundant background with the other two antigens (calretinin and COX-2), which hindered interpretation of the specific reaction. The cost of ImmPRESS was 25% lower than for ENVISION+trade mark. Based on these results, ImmPRESS is a good polymer-based detection system for routine immunohistochemistry.
Ramos-Vara
José A
JA
Purdue University, Animal Disease Diagnostic Laboratory, School of Veterinary Medicine, 406 South University, West Lafayette, IN 47905, USA. ramosja@purdue.edu
Miller
Margaret A
MA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000276:immunology*; D001230:Aspergillus / Q000276:immunology; D001710:Biotin / Q000737:chemistry / Q000276:immunology*; D002139:Caliciviridae / Q000276:immunology; D002415:Cats; D004285:Dogs; D005557:Formaldehyde; D007150:Immunohistochemistry / Q000379:methods*; D051379:Mice; D016612:Paraffin Embedding; D011108:Polymers / Q000737:chemistry*; D011817:Rabbits; D011933:Reagent Kits, Diagnostic / Q000191:economics; D012680:Sensitivity and Specificity; D013194:Staining and Labeling; D016707:Tissue Fixation; D014902:West Nile virus / Q000276:immunology
D000911:Antibodies, Monoclonal; D000941:Antigens; D011108:Polymers; D011933:Reagent Kits, Diagnostic; D005557:Formaldehyde; D001710:Biotin