[New methods in the investigation of brain hypoxia].
The main challenge is the investigation of mechanism for apoptosis research and the drug development. Mitochondria have a key position in the production of reactive oxygen species and in the evolution of apoptosis. More possible pathway will be known with the apoptosis investigation. For development of neuroprotective molecules could give strategies the investigation of apoptosis. Exact knowledge of apoptosis provides the possibility to screen new neuroprotective molecules. We developed a research assay, that could provide quantitative and qualitative data about the free radical production and the mitochondrial transmembrane potential using confocal microscopy. Thus, we could screen drug candidate, neuroprotective molecules.
Szilágyi
Géza
G
Sejtbiológiai Laboratórium, Agyérbetegségek Országos Központja, Semmelweis Egyetem, Vascularis Neurol6giai Tanszéki Csoport, Országos Pszichiátriai és Neurológiai Intézet, Budapest. szilagyi@radi.sote.hu
Nagy
Zoltán
Z
D000818:Animals; D017209:Apoptosis; D001681:Biological Assay / Q000379:methods*; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical; D006801:Humans; D002534:Hypoxia, Brain / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D053078:Membrane Potential, Mitochondrial; D018613:Microscopy, Confocal; D018696:Neuroprotective Agents / Q000494:pharmacology; D018384:Oxidative Stress; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism
D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species