title: Improved collision-induced dissociation analysis of peptides by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry through 3-sulfobenzoic acid succinimidyl ester labeling.
abstract: The sulfonation reagent, a succinimidyl ester of 3-sulfobenzoic acid, has been synthesized for effective peptide sequencing. It is capable of incorporating an additional mobile proton into the peptide backbone, thus, facilitating efficient collision-induced dissociation. This reagent is easily and inexpensively prepared in short time. Tandem mass spectra of the guanidinated and reagent-sulfonated peptides consist mainly of the y-ion series with higher intensities than those observed for solely guanidinated peptides. These enhanced tandem MS attributes significantly improved MASCOT total-ion scores, thus, allowing more confident peptide sequencing. This derivatization was also very effective for the analysis of tryptic digest of human blood serum proteins separated by two-dimensional gel electrophoresis. When used in LC-MALDI/MS/MS format, this type of derivatization does not adversely affect chromatographic efficiencies.
authors.affiliation: Department of Chemistry, Indiana University, Bloomington, Indiana 47405, USA.
authors.forename: William R
authors.initials: WR
authors.lastname: Alley
authors.forename: Yehia
authors.initials: Y
authors.lastname: Mechref
authors.forename: Iveta
authors.initials: I
authors.lastname: Klouckova
authors.forename: Milos V
authors.initials: MV
authors.lastname: Novotny
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016632:Apolipoprotein A-I / Q000737:chemistry; D001557:Benzenesulfonates / Q000737:chemistry / Q000494:pharmacology*; D001798:Blood Proteins / Q000737:chemistry*; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D004952:Esters / Q000737:chemistry*; D006736:Horses / Q000378:metabolism; D006801:Humans; D007834:Lasers; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D009211:Myoglobin / Q000378:metabolism; D010455:Peptides / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: C521207:3-sulfobenzoic acid succinimidyl ester; D016632:Apolipoprotein A-I; D001557:Benzenesulfonates; D001798:Blood Proteins; D004952:Esters; D009211:Myoglobin; D010455:Peptides