title: MPAI (mass probes aided ionization) method for total analysis of biomolecules by mass spectrometry.
abstract: We have designed and synthesized various mass probes, which enable us to effectively ionize various molecules to be detected with mass spectrometry. We call the ionization method using mass probes the "MPAI (mass probes aided ionization)" method. We aim at the sensitive detection of various biological molecules, and also the detection of bio-molecules by a single mass spectrometry serially without changing the mechanical settings. Here, we review mass probes for small molecules with various functional groups and mass probes for proteins. Further, we introduce newly developed mass probes for proteins for highly sensitive detection.
authors.affiliation: Department of Applied Chemistry, Keio University, Yokohama, Kanagawa, Japan.
authors.forename: Aki
authors.initials: A
authors.lastname: Honda
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Hayashi
authors.forename: Hiroki
authors.initials: H
authors.lastname: Hifumi
authors.forename: Yuya
authors.initials: Y
authors.lastname: Honma
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Tanji
authors.forename: Naoko
authors.initials: N
authors.lastname: Iwasawa
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Koji
authors.initials: K
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D006801:Humans; D007202:Indicators and Reagents; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D009696:Nucleic Acids / Q000032:analysis*; D009711:Nucleotides / Q000032:analysis; D011506:Proteins / Q000032:analysis*
chemical_list: D007202:Indicators and Reagents; D009696:Nucleic Acids; D009711:Nucleotides; D011506:Proteins