[Determination of plumbagin in different parts of Plumbago zeylanica by RP-HPLC].
OBJECTIVE
To develop a RP-HPLC method to determine plumbagin in Plumbago zeylanica, and to investigate contents of plumbagin in different parts of. P. zeylanica.


METHODS
The analysis was carried out at 30 degrees C on a Kromasil C18, column eluted with a mobile phase consisting of a mixture of methanol-water (65: 35). The flow rate was 1 mL x min(-1), the detector wavelength was 213 nm.


RESULTS
The calibration curve was linear within the concentration ranges of 0.020 8-0. 104 microg (r = 0. 9999). The average recovery was 98.7%. The contents in the root, stem and leaf were 0.394 5%, 0.050 8%, 0.031 4% respectively.


CONCLUSIONS
This method is simple, accurate, replicate and suitable for the determination of plumbagin in P. zeylanica.
Liu
Yuan
Y
Ethnic Pharmaceutical Institute of Southwest University for Nationalities, Chengdu 610041, China.
Deng
Fang
F
Liu
Chao
C
Meng
Qing-Yan
QY
Gao
Ze-Wen
ZW
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009285:Naphthoquinones / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D029606:Plumbaginaceae / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results
D009285:Naphthoquinones; C014758:plumbagin