Identification of the glycosyl transferase required for synthesis of the principal glycolipid characteristic of heterocysts of Anabaena sp. strain PCC 7120.
Nitrogenase is oxygen-labile. Cyanobacterial heterocysts can fix N(2) in an oxic milieu because their interior is micro-oxic, for which the glycolipid layer of the heterocyst envelope is required. ORF all5341 of the Anabaena sp. genome predicts a glycosyl transferase. An insertional mutant of all5341 synthesized only a nonglycosylated form of heterocyst envelope glycolipid, and lacked a glycolipid layer. All5341 appears to be the transferase required to glycosylate the glycolipid aglycone.
Awai
Koichiro
K
Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA.
Wolk
C Peter
CP
D017033:Anabaena / Q000201:enzymology* / Q000378:metabolism / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D016680:Genome, Bacterial; D006017:Glycolipids / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D016695:Glycosyltransferases / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D008055:Lipids / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D016254:Mutagenesis, Insertional; D016366:Open Reading Frames
D001426:Bacterial Proteins; D006017:Glycolipids; D008055:Lipids; D016695:Glycosyltransferases