title: Transglucosylation of ascorbic acid to ascorbic acid 2-glucoside by a recombinant sucrose phosphorylase from Bifidobacterium longum.
abstract: A novel transglycosylation reaction from sucrose to L-ascorbic acid by a recombinant sucrose phosphorylase from Bifidobacterium longum was used to produce a stable L-ascorbic acid derivative. The major product was detected by HPLC, and confirmed to be 2-O-alpha-D-glucopyranosyl-L-ascorbic acid by LC-MS/MS analysis.
authors.affiliation: Department of Food Science and Biotechnology, Kyonggi University, Suwon, South Korea.
authors.forename: Taeyeon
authors.initials: T
authors.lastname: Kwon
authors.forename: Cheong Tae
authors.initials: CT
authors.lastname: Kim
authors.forename: Jong-Hoon
authors.initials: JH
authors.lastname: Lee
mesh_terms: D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000737:chemistry*; D001644:Bifidobacterium / Q000201:enzymology* / Q000235:genetics; D004789:Enzyme Activation; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology; D005964:Glucosyltransferases / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry; D013395:Sucrose / Q000737:chemistry*
chemical_list: D011994:Recombinant Proteins; C065378:ascorbic acid 2-O-glucoside; D013395:Sucrose; D005964:Glucosyltransferases; C025901:sucrose phosphorylase; D001205:Ascorbic Acid