Left-right dynein motor implicated in selective chromatid segregation in mouse cells.
During cell division, copies of mouse chromosome 7 are segregated selectively or randomly to daughter cells depending on the cell type. The mechanism for differential segregation is unknown. Because mouse left-right dynein (LRD) gene mutations result in randomization of visceral organs' laterality, we hypothesized that LRD may also function in selective chromatid segregation. Indeed, upon knock-down by RNA interference methods, LRD depletion disrupts biased segregation. LRD messenger RNA presence or absence correlates with the observed segregation patterns. This work supports the claim that LRD functions in a mechanism for selective chromatid segregation.
Armakolas
Athanasios
A
Gene Regulation and Chromosome Biology Laboratory, Center for Cancer Research, National Cancer Institute at Frederick, Post Office Box B, Frederick, MD 21702-1201, USA.
Klar
Amar J S
AJ
D000818:Animals; D056444:Axonemal Dyneins; D019521:Body Patterning; D002460:Cell Line; D019070:Cell Lineage; D002842:Chromatids / Q000502:physiology*; D020090:Chromosome Segregation; D004261:DNA Replication; D004398:Dyneins / Q000235:genetics* / Q000502:physiology*; D004475:Ectoderm / Q000166:cytology*; D053595:Embryonic Stem Cells / Q000166:cytology*; D004707:Endoderm / Q000166:cytology*; D007399:Interphase; D051379:Mice; D008938:Mitosis; D009154:Mutation; D034622:RNA Interference; D011995:Recombination, Genetic
C402391:Dnah11 protein, mouse; D056444:Axonemal Dyneins; D004398:Dyneins