title: Simultaneous determination of metformin and glipizide in human plasma by liquid chromatography-tandem mass spectrometry.
abstract: A method has been developed for the simultaneous quantification of metformin (I) and glipizide (II) in human plasma. It is based on high-performance liquid chromatography with electrospray ionization tandem mass (LC-ESI-MS/MS) spectrometric detection in positive ionization mode. Phenformin (III) and gliclazide (IV) were used as internal standards for I and II, respectively. The MS/MS detection was performed in multiple reaction monitoring (MRM) mode. The precursor-product ion combinations of m/z 130 --> 71, 446 --> 321, 206 --> 60 and 324 --> 127 were used to quantify I, II, III and IV, respectively. This method was validated in the concentration ranges of 0.02-4 microg/mL for I and 0.004-0.8 microg/mL for II. It was utilized to support a clinical pharmacokinetic study after single dose oral administration of a combination of I and II.
authors.affiliation: Shanghai Institute of Pharmaceutical Industry, 1111 Zhongshan North One Road, Shanghai 200437, People's Republic of China.
authors.forename: Cun-Gang
authors.initials: CG
authors.lastname: Ding
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhou
authors.forename: Qing-Hua
authors.initials: QH
authors.lastname: Ge
authors.forename: Xiao-Jin
authors.initials: XJ
authors.lastname: Zhi
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Ma
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D005913:Glipizide / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D008297:Male; D008687:Metformin / Q000097:blood* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D008687:Metformin; D005913:Glipizide