Differential expression of 3beta-hydroxysteroid dehydrogenase mRNA in rat testes exposed to endocrine disruptors.
Expression of 3beta-hydroxysteroid dehydrogenase (3beta-HSD) is mainly found in the Leydig cells from which steroid hormones are biosynthesized in the testes. To investigate whether endocrine disruptors affect the microenvironment of the testes, the mRNA expression of 3beta-HSD as a molecular marker for androgen biosynthesis was analyzed in rat testes exposed to several endocrine disruptors using a reverse transcription-polymerase chain reaction technique. Testosterone [50, 200 and 1,000 microg/kg body weight (BW)], flutamide (1, 5 and 25 mg/kg BW), ketoconazole (0.2, 1, 5 and 25 mg/kg BW), diethylhexyl phthalate (10, 50 and 250 mg/kg BW), nonylphenol (10, 50, 100 and 250 mg/kg BW), octylphenol (10, 50 and 250 mg/kg BW), and diethylstilbestrol (10, 20 and 40 microg/kg BW) were orally administered to 4-week-old Sprague-Dawley rats for 3 weeks daily. Although testosterone at a low dose (50 microg/kg/day) increased the expression of 3beta-HSD mRNA, it was significantly decreased in the rats treated with 200 or 1,000 microg/kg/day testosterone compared with the control group (P<0.05). Furthermore, ketoconazole, diethylhexyl phthalate, nonylphenol, octylphenol and diethylstilbestrol caused significant downregulation of 3beta-HSD mRNA in the testes at all doses (P<0.05). However, flutamide remarkably increased the level of 3beta-HSD mRNA in the testes (P<0.05). These results suggest that endocrine disruptors may influence androgen biosynthesis in the testes by alteration of 3beta-HSD mRNA expression.
Kim
Hwan-Hee
HH
College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Korea.
Kwak
Dong-Hoon
DH
Yon
Jung-Min
JM
Baek
In-Jeoung
IJ
Lee
Se-Ra
SR
Lee
Jeung-Eun
JE
Nahm
Sang-Soep
SS
Jeong
Jae-Hwang
JH
Lee
Beom Jun
BJ
Yun
Young Won
YW
Nam
Sang-Yoon
SY
D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D052244:Endocrine Disruptors / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D007985:Leydig Cells / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D015415:Biomarkers; D052244:Endocrine Disruptors; D012333:RNA, Messenger; D015096:3-Hydroxysteroid Dehydrogenases