Transglucosylation of ascorbic acid to ascorbic acid 2-glucoside by a recombinant sucrose phosphorylase from Bifidobacterium longum.
A novel transglycosylation reaction from sucrose to L-ascorbic acid by a recombinant sucrose phosphorylase from Bifidobacterium longum was used to produce a stable L-ascorbic acid derivative. The major product was detected by HPLC, and confirmed to be 2-O-alpha-D-glucopyranosyl-L-ascorbic acid by LC-MS/MS analysis.
Kwon
Taeyeon
T
Department of Food Science and Biotechnology, Kyonggi University, Suwon, South Korea.
Kim
Cheong Tae
CT
Lee
Jong-Hoon
JH
D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000737:chemistry*; D001644:Bifidobacterium / Q000201:enzymology* / Q000235:genetics; D004789:Enzyme Activation; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology; D005964:Glucosyltransferases / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry; D013395:Sucrose / Q000737:chemistry*
D011994:Recombinant Proteins; C065378:ascorbic acid 2-O-glucoside; D013395:Sucrose; D005964:Glucosyltransferases; C025901:sucrose phosphorylase; D001205:Ascorbic Acid