title: Specific radiation damage to acidic residues and its relation to their chemical and structural environment.
abstract: Intense synchrotron radiation produces specific structural and chemical damage to crystalline proteins even at 100 K. Carboxyl groups of acidic residues (Glu, Asp) losing their definition is one of the major effects observed. Here, the susceptibilities to X-ray damage of acidic residues in tetrameric malate dehydrogenase from Haloarcula marismortui are investigated. The marked excess of acidic residues in this halophilic enzyme makes it an ideal target to determine how specific damage to acidic residues is related to their structural and chemical environment. Four conclusions are drawn. (i) Acidic residues interacting with the side-chains of lysine and arginine residues are less affected by radiation damage than those interacting with serine, threonine and tyrosine side-chains. This suggests that residues with higher pK(a) values are more vulnerable to damage than those with a lower pK(a). However, such a correlation was not found when calculated pK(a) values were inspected. (ii) Acidic side-chains located in the enzymatic active site are the most radiation-sensitive ones. (iii) Acidic residues in the internal cavity formed by the four monomers and those involved in crystal contacts appear to be particularly susceptible. (iv) No correlation was found between radiation susceptibility and solvent accessibility.
authors.affiliation: Laboratoire de Biophysique Moléculaire, Institut de Biologie Structurale J.-P. Ebel, CEA CNRS UJF, 41 Rue Jules Horowitz, 38027 Grenoble CEDEX 1, France. fioravan@esrf.fr
authors.forename: Emanuela
authors.initials: E
authors.lastname: Fioravanti
authors.forename: Frédéric M D
authors.initials: FM
authors.lastname: Vellieux
authors.forename: Patricia
authors.initials: P
authors.lastname: Amara
authors.forename: Dominique
authors.initials: D
authors.lastname: Madern
authors.forename: Martin
authors.initials: M
authors.lastname: Weik
mesh_terms: D000596:Amino Acids / Q000737:chemistry* / Q000528:radiation effects*; D003198:Computer Simulation; D018360:Crystallography, X-Ray / Q000379:methods*; D004307:Dose-Response Relationship, Radiation; D019614:Haloarcula marismortui / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008291:Malate Dehydrogenase / Q000737:chemistry* / Q000528:radiation effects* / Q000648:ultrastructure; D008956:Models, Chemical; D008958:Models, Molecular; D011487:Protein Conformation / Q000528:radiation effects; D011489:Protein Denaturation / Q000528:radiation effects; D011829:Radiation Dosage; D012996:Solutions; D012997:Solvents / Q000737:chemistry; D013329:Structure-Activity Relationship; D014965:X-Rays
chemical_list: D000596:Amino Acids; D012996:Solutions; D012997:Solvents; D008291:Malate Dehydrogenase