PPAR-gamma agonist, ciglitazone, increases pigmentation and migration of human melanocytes.
Peroxisome proliferator-activated receptors (PPARs) play an important role in cellular responses. It was reported that three subtypes of PPAR are expressed in human melanocytes. In this study, we investigated the effects of the PPAR-gamma agonist, ciglitazone, on pigmentation and migration of human melanocytes. Ciglitazone stimulated the melanin content of cells and cultured skin. This increase in pigmentation was due to the stimulation of tyrosinase activity and expression of tyrosinase and microphthalmia-associated transcription factor protein of the melanocytes. Migration was increased after ciglitazone treatment, which was observed by the Boyden chamber checkerboard analysis and a scratch motility assay. These results suggest the regulatory role of PPAR-gamma in pigmentation and migration of human melanocytes.
Lee
Joong Sun
JS
Department of Dermatology, Ajou University School of Medicine, Suwon, Korea.
Choi
You Mi
YM
Kang
Hee Young
HY
D002465:Cell Movement / Q000502:physiology*; D015870:Gene Expression; D006801:Humans; D008543:Melanins / Q000378:metabolism; D008544:Melanocytes / Q000378:metabolism / Q000502:physiology*; D051739:Microphthalmia-Associated Transcription Factor / Q000378:metabolism; D014442:Monophenol Monooxygenase / Q000378:metabolism; D009924:Organ Culture Techniques; D047495:PPAR gamma / Q000819:agonists / Q000502:physiology*; D010858:Pigmentation / Q000502:physiology*; D012879:Skin Physiological Phenomena; D045162:Thiazolidinediones / Q000494:pharmacology
D008543:Melanins; D051739:Microphthalmia-Associated Transcription Factor; D047495:PPAR gamma; D045162:Thiazolidinediones; D014442:Monophenol Monooxygenase; C039671:ciglitazone