title: Measurement of lysophospholipase D/autotaxin activity in human serum samples.
abstract: OBJECTIVE
  Lysophospholipase D (lysoPLD)/autotaxin regulates the blood levels of lysophosphatidic acid (LPA), and we designed a serum lysoPLD assay for use in clinical laboratory testing.
  
  
  METHODS
  LysoPLD activity was assessed based on the amount of choline released with lysophosphatidylcholine as the substrate.
  
  
  CONCLUSIONS
  It was confirmed that the lysoPLD assay can be applied to clinical laboratory testing. The serum lysoPLD activity in healthy female subjects was significantly higher than that in the male subjects.
authors.affiliation: Department of Clinical Laboratory, The University of Tokyo Hospital, Tokyo, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Nakamura
authors.forename: Ryunosuke
authors.initials: R
authors.lastname: Ohkawa
authors.forename: Shigeo
authors.initials: S
authors.lastname: Okubo
authors.forename: Minoru
authors.initials: M
authors.lastname: Tozuka
authors.forename: Mitsumasa
authors.initials: M
authors.lastname: Okada
authors.forename: Shinya
authors.initials: S
authors.lastname: Aoki
authors.forename: Junken
authors.initials: J
authors.lastname: Aoki
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Arai
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ikeda
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yatomi
mesh_terms: D019411:Clinical Laboratory Techniques; D005260:Female; D006801:Humans; D008297:Male; D009097:Multienzyme Complexes / Q000097:blood*; D043264:Phosphodiesterase I / Q000097:blood*; D010727:Phosphoric Diester Hydrolases / Q000097:blood*; D011755:Pyrophosphatases / Q000097:blood*; D012680:Sensitivity and Specificity; D012737:Sex Factors; D000099189:Lysophospholipase D
chemical_list: D009097:Multienzyme Complexes; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases; D011755:Pyrophosphatases; D000099189:Lysophospholipase D