title: Binding affinity of proanthocyanidin from waste Pinus radiata bark onto proline-rich bovine achilles tendon collagen type I.
abstract: Binding affinity of proanthocyanidin (PA) purified from Pinus radiata bark waste onto bovine Achilles tendon collagen (type I) was studied. Adsorption of PA onto the collagen was optimized by examining pH, contact time and temperature. The adsorption was pH-dependent. The maximum adsorption capacity (Q(0)) of PA on collagen was found to be 211 mg g(-1) using the Langmuir isotherm. Comparison between two adsorbents also showed that collagen had higher adsorptivity of approximately 20% more than PVPP (polyvinyl polypyrrolidone). The high affinity between PA and collagen was further confirmed in solvent solubility experiments. The observed solvent resistance was thought to be mainly due to a hydrophobic stacking mechanism reinforced by hydrogen bonding. FT-IR spectra clearly indicated the presence of PA adsorbed on collagen. The results have interesting implications that PA can be a good protective agent for collagen against collagenase and other enzymes.
authors.affiliation: Department of Advanced Organic Materials Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea.
authors.forename: C S
authors.initials: CS
authors.lastname: Ku
authors.forename: M
authors.initials: M
authors.lastname: Sathishkumar
authors.forename: S P
authors.initials: SP
authors.lastname: Mun
mesh_terms: D000125:Achilles Tendon / Q000737:chemistry*; D000327:Adsorption; D000818:Animals; D002417:Cattle; D024042:Collagen Type I / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste / Q000032:analysis*; D007700:Kinetics; D028223:Pinus / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry*; D044945:Proanthocyanidins / Q000737:chemistry*; D011392:Proline / Q000032:analysis; D011485:Protein Binding; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D024042:Collagen Type I; D007220:Industrial Waste; D044945:Proanthocyanidins; C013221:proanthocyanidin; D011392:Proline