Bioinformatics and microbial biodiversity: analysis of vibrios by the GenEnv system.
Sequence-based approaches to prokaryotic systematics and typing represent a modern and promising strategy in epidemiology and environmental microbiology. GenEnv, a database-driven system for bacterial typing, was developed in order to provide user friendly tools for supporting biomolecular analysis of bacteria. The family Vibrionaceae represents a heterogeneous taxon of aquatic microrganisms, harbouring a plethora of genomes currently analyzed by different molecular techniques. Under the query "Vibrio", GenEnv retrieved 256 organisms, included in a total number of 19 families. Overall, 548 sequences, comprising 16S rRNA (n=402), rpoB (n=1), gyrB (n=145) were available. In addition, GenEnv system allowed primer design, homology analysis and restriction maps, for immediate applications to the study of Vibrionaceae.
Paparini
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A
Department of Health Sciences, Public Health Unit, University Institute of Movement Sciences, Rome, Italy.
Santoni
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D
Romano Spica
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V
D015373:Bacterial Typing Techniques; D044822:Biodiversity; D019295:Computational Biology / Q000379:methods*; D030541:Databases, Genetic; D004812:Epidemiologic Methods; D005798:Genes, Bacterial; D012336:RNA, Ribosomal, 16S; D014733:Vibrio / Q000235:genetics* / Q000472:pathogenicity; D014871:Water Microbiology
D012336:RNA, Ribosomal, 16S