Gas-related impact of pneumoperitoneum on systemic wound healing.
BACKGROUND
The aim of the present study was to investigate the gas-dependent effects of pneumoperitoneum on wound healing distant from the abdomen.


METHODS
Dorsal skin incisions were performed in 54 male Sprague-Dawley rats. A CO(2) or a helium pneumoperitoneum of 3 mmHg was maintained before, with an overall duration of 30 min (each n = 18). Rats in the control group received laparotomy only (n = 18). Animals were killed after 5 and 10 days. The infiltration of macrophages (CD 68), expression of matrix metalloproteinase 13 (MMP-13) and collagen I/III ratios were analysed in the dorsal skin wounds.


RESULTS
Both after 5 and 10 days, infiltration of macrophages and expression of MMP-13 were greatest after helium pneumoperitoneum. After 5 days, collagen I/III ratio was significantly increased in the helium group. After 10 days, collagen I/III ratio was lowest in the CO(2) group.


CONCLUSIONS
Our results suggest beneficial effects on systemic wound healing for helium pneumoperitoneum as compared to CO(2).
Rosch
R
R
Department of Surgery, University Hospital Aachen, Aachen, Germany. r.rosch@chir.rwth-aachen.de
Junge
K
K
Binnebösel
M
M
Mirgartz
N
N
Klinge
U
U
Schumpelick
V
V
D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D002245:Carbon Dioxide; D024042:Collagen Type I / Q000032:analysis; D024061:Collagen Type III / Q000032:analysis; D062109:Dermatologic Surgical Procedures; D006371:Helium; D007124:Immunoenzyme Techniques; D008262:Macrophage Activation / Q000502:physiology; D008264:Macrophages / Q000473:pathology; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000032:analysis; D011028:Pneumoperitoneum, Artificial; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000473:pathology*; D014945:Wound Healing / Q000502:physiology*; D000097382:CD68 Molecule
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D002245:Carbon Dioxide; D024042:Collagen Type I; D024061:Collagen Type III; D006371:Helium; D053509:Matrix Metalloproteinase 13; C539355:CD68 protein, rat; D000097382:CD68 Molecule