title: A bipartite sequence motif induces translation reinitiation in feline calicivirus RNA.
abstract: The mechanism leading to reinitiation of translation after termination of protein synthesis in eukaryotes has not yet been resolved in detail. One open question concerns the way the post-termination ribosome is tethered to the mRNA to allow binding of the necessary initiation factors. In caliciviruses, a family of positive strand RNA viruses, the capsid protein VP2 is translated via a termination/reinitiation process. VP2 of the feline calicivirus is encoded in the 3'-terminal open reading frame 3 (ORF3) that overlaps with the preceding ORF2 by four nucleotides. In transient expression studies, the efficiency of VP2 expression was 20 times lower than that of the ORF2 proteins. The close vicinity of the ORF2 termination signal and the ORF3 AUG codon was crucial, whereas the AUG could be replaced by alternative codons. Deletion mapping revealed that the 3'-terminal 69 nucleotides of ORF2 are crucial for VP2 expression. This sequence contains two essential sequence motifs. The first motif is conserved among caliciviruses and complementary to part of the 18 S rRNA. In conclusion, VP2 is expressed in a translation termination/reinitiation process that is special because it requires a sequence element that could prevent dissociation of post-termination ribosomes via hybridization with 18 S rRNA.
authors.affiliation: Institut für Immunologie, Friedrich-Loeffler-Institut, D-72001 Tübingen, Germany.
authors.forename: Christine
authors.initials: C
authors.lastname: Luttermann
authors.forename: Gregor
authors.initials: G
authors.lastname: Meyers
mesh_terms: D001483:Base Sequence; D017927:Calicivirus, Feline / Q000235:genetics*; D036022:Capsid Proteins / Q000235:genetics*; D003062:Codon; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D014176:Protein Biosynthesis; D012337:RNA, Ribosomal, 18S / Q000737:chemistry; D012367:RNA, Viral / Q000737:chemistry*
chemical_list: D036022:Capsid Proteins; D003062:Codon; D012337:RNA, Ribosomal, 18S; D012367:RNA, Viral