Shape-engineered vascular endothelial cells: nitric oxide production, cell elasticity, and actin cytoskeletal features.
Single cell shape determines cellular functions. Therefore, control of cell shape is of considerable importance for the tissue engineering field. This study was designed to assess the effect of surface-induced shaping of vascular endothelial cells (ECs) on the intracellular nitric oxide (NO) production level, the cell elasticity, and cytoskeletal (CSK) features on shape-engineered ECs (round, 90, 120 microm diameter; spindle-shaped, 20, 30, 40 microm width) prepared on a photolithographically microprocessed surface. Intracellular NO production was measured using a microscopic spectrometer with diaminofluorescein diacetate probe. Cell elasticity and actin CSK features were analyzed through microindentation measurement and fluorescence observations with fluorescence and atomic force microscopy. Results showed that spindle-shaped cells exhibited lower NO production, higher cell stiffness, and denser actin stress fibers than the round and nonrestrictedly cultured control cells. Relations between cell shape with NO production, cell elasticity, and actin CSK features were discussed.
Kidoaki
Satoru
S
Division of Biomedical Engineering, Graduate School of Medicine, Kyushu University, Fukuoka 812-8582, Japan.
Matsuda
Takehisa
T
D000199:Actins / Q000378:metabolism*; D048430:Cell Shape; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000378:metabolism*; D004548:Elasticity; D042783:Endothelial Cells / Q000166:cytology*; D005453:Fluorescence; D006801:Humans; D009569:Nitric Oxide / Q000096:biosynthesis*; D023822:Tissue Engineering
D000199:Actins; D009569:Nitric Oxide