title: Cell cycle progression and de novo centriole assembly after centrosomal removal in untransformed human cells.
abstract: How centrosome removal or perturbations of centrosomal proteins leads to G1 arrest in untransformed mammalian cells has been a mystery. We use microsurgery and laser ablation to remove the centrosome from two types of normal human cells. First, we find that the cells assemble centrioles de novo after centrosome removal; thus, this phenomenon is not restricted to transformed cells. Second, normal cells can progress through G1 in its entirety without centrioles. Therefore, the centrosome is not a necessary, integral part of the mechanisms that drive the cell cycle through G1 into S phase. Third, we provide evidence that centrosome loss is, functionally, a stress that can act additively with other stresses to arrest cells in G1 in a p38-dependent fashion.
authors.affiliation: Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01605, USA.
authors.forename: Yumi
authors.initials: Y
authors.lastname: Uetake
authors.forename: Jadranka
authors.initials: J
authors.lastname: Loncarek
authors.forename: Joshua J
authors.initials: JJ
authors.lastname: Nordberg
authors.forename: Christopher N
authors.initials: CN
authors.lastname: English
authors.forename: Sabrina
authors.initials: S
authors.lastname: La Terra
authors.forename: Alexey
authors.initials: A
authors.lastname: Khodjakov
authors.forename: Greenfield
authors.initials: G
authors.lastname: Sluder
mesh_terms: D001973:Bromodeoxyuridine / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000032:analysis; D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D002478:Cells, Cultured; D002502:Centrioles / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D018385:Centrosome / Q000502:physiology*; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D016193:G1 Phase / Q000502:physiology; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D008027:Light; D008854:Microscopy, Electron; D011725:Pyridines / Q000494:pharmacology; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D002135:Calcium-Binding Proteins; D002868:Chromosomal Proteins, Non-Histone; D004791:Enzyme Inhibitors; D007093:Imidazoles; D011725:Pyridines; C056608:caltractin; D048051:p38 Mitogen-Activated Protein Kinases; D001973:Bromodeoxyuridine; C093642:SB 203580