Evaluation of genetic markers and molecular typing methods for prediction of sources of Campylobacter jejuni and C. coli infections.
Campylobacter jejuni and C. coli isolates from poultry, cattle, and humans were studied using pulsed-field gel electrophoresis (PFGE) and PCR of candidate livestock-associated marker genes. Human isolates showed 5.7 and 61% overlap with cattle and poultry isolates, respectively, by use of PFGE. No unambiguous association was found between marker genes (the Cj1321 and Cj1324 genes) and livestock-associated isolates.
Kärenlampi
Rauni
R
Department of Food and Environmental Hygiene, Faculty of Veterinary Medicine, P.O. Box 66, FI-00014 University of Helsinki, and HUSLAB, Helsinki University Central Hospital Laboratory, Finland. rauni.karenlampi@helsinki.fi
Rautelin
Hilpi
H
Hänninen
Marja-Liisa
ML
D000818:Animals; D015373:Bacterial Typing Techniques; D002169:Campylobacter Infections / Q000382:microbiology* / Q000662:veterinary; D017000:Campylobacter coli / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016123:Campylobacter jejuni / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D002645:Chickens / Q000382:microbiology; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D005243:Feces / Q000382:microbiology; D005506:Food Contamination; D005516:Food Microbiology; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D008460:Meat / Q000382:microbiology; D011201:Poultry Diseases / Q000382:microbiology
D005819:Genetic Markers; D015252:Deoxyribonucleases, Type II Site-Specific; C029312:GGTACC-specific type II deoxyribonucleases