title: Rearrangement of a conditional allele regardless of inheritance of a Cre recombinase transgene.
abstract: To generate conditional gene knockouts in osteoblasts, we previously developed transgenic mice in which Cre recombinase cDNA was cloned downstream of a 3.6 or 2.3 kb fragment of the rat Col1a1 promoter (Col3.6-Cre and Col2.3-Cre, respectively). Col-Cre mice were bred with mice in which exon 4 of the Igf1 gene is flanked by loxP sites. Mating units were arranged such that either the male or the female breeder transmitted the Col-Cre transgenes. Progeny were evaluated for Cre-mediated Igf1 gene rearrangement. We found that the loxP-flanked Igf1 locus was rearranged in the absence of inheritance of the Cre transgene. The incidence was 50 and 28% with Col2.3-Cre and Col3.6-Cre females, respectively, and 15 and 18% with Col2.3-Cre and Col3.6-Cre males, respectively.
authors.affiliation: Department of Medicine, University of Connecticut Health Center, Farmington, Connecticut 06030-1850, USA.
authors.forename: Rebecca L
authors.initials: RL
authors.lastname: Cochrane
authors.forename: Stephen H
authors.initials: SH
authors.lastname: Clark
authors.forename: Adam
authors.initials: A
authors.lastname: Harris
authors.forename: Barbara E
authors.initials: BE
authors.lastname: Kream
mesh_terms: D000483:Alleles; D000818:Animals; D005260:Female; D040582:Inheritance Patterns; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism; D019426:Integrases / Q000235:genetics*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D019076:Transgenes
chemical_list: D007334:Insulin-Like Growth Factor I; C045073:Cre recombinase; D019426:Integrases