Evaluation of immunodiagnostics for toxocarosis in experimental porcine cysticercosis.
We assessed whether immunodiagnostic tests for cysticercosis can cross-react with the currently available immunodiagnostic tests for Toxocara canis in an established animal model for cysticercosis infection in pigs, known host for Toxocara. We examined by TES-enzyme-linked immunosorbent test and immunoblot assay for toxocarosis and cysticercosis the baseline and final follow-up sera of 10 pigs, before and after (3 months) infection with Taenia solium. After successful cysticercosis infection, the nine evaluable pigs became seropositive to T. solium (enzyme-linked immunoelectrotransfer blot assay), but did remain seronegative for Toxocara in both assays, documenting the lack of cross-reactivity with anti-T. solium antibodies in both T. canis assays. These findings should help clinicians better interpret serology for toxocariosis and cysticercosis in endemic areas for both helminth infections.
García
Hector H
HH
Department of Microbiology, Universidad Peruana Cayetano Heredia, San Martin de Porres, Lima, Peru.
Cancrini
Gabriella
G
Bartalesi
Filippo
F
Rodriguez
Silvia
S
Jimenez
Juan A
JA
Roldan
William
W
Mantella
Antonia
A
Nicoletti
Alessandra
A
Bartoloni
Alessandro
A
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology; D003429:Cross Reactions / Q000276:immunology; D003551:Cysticercosis / Q000175:diagnosis* / Q000276:immunology; D004195:Disease Models, Animal; D019353:Endemic Diseases; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D015151:Immunoblotting / Q000379:methods; D013552:Swine; D017166:Toxocara canis / Q000276:immunology*; D014120:Toxocariasis / Q000175:diagnosis* / Q000276:immunology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth