title: Picobiliphytes: a marine picoplanktonic algal group with unknown affinities to other eukaryotes.
abstract: Environmental sequencing has revealed unimagined diversity among eukaryotic picoplankton. A distinct picoplanktonic algal group, initially detected from 18S ribosomal DNA (rDNA) sequences, was hybridized with rRNA-targeted probes, detected by tyramide signal amplification-fluorescent in situ hybridization, and showed an organelle-like body with orange fluorescence indicative of phycobilins. Using this fluorescence signal, cells were sorted by flow cytometry and probed. Hybridized cells contained a 4',6'-diamidino-2-phenylindole-stained organelle resembling a plastid with a nucleomorph. This suggests that they may be secondary endosymbiotic algae. Pending the isolation of living cells and their formal description, these algae have been termed picobiliphytes.
authors.affiliation: Station Biologique de Roscoff, UMR 7144 CNRS and Université Pierre et Marie Curie, Boîte Postale 74, 29682 Roscoff Cedex, France. not@icm.csic.es
authors.forename: Fabrice
authors.initials: F
authors.lastname: Not
authors.forename: Klaus
authors.initials: K
authors.lastname: Valentin
authors.forename: Khadidja
authors.initials: K
authors.lastname: Romari
authors.forename: Connie
authors.initials: C
authors.lastname: Lovejoy
authors.forename: Ramon
authors.initials: R
authors.lastname: Massana
authors.forename: Kerstin
authors.initials: K
authors.lastname: Töbe
authors.forename: Daniel
authors.initials: D
authors.lastname: Vaulot
authors.forename: Linda K
authors.initials: LK
authors.lastname: Medlin
mesh_terms: D001499:Bayes Theorem; D004275:DNA, Ribosomal / Q000235:genetics; D056890:Eukaryota / Q000145:classification / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification; D005434:Flow Cytometry; D005453:Fluorescence; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D015388:Organelles / Q000648:ultrastructure; D052979:Phycobiliproteins / Q000032:analysis; D010802:Phylogeny; D010839:Phytoplankton / Q000145:classification / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification; D012621:Seasons; D012623:Seawater / Q000382:microbiology*; D013559:Symbiosis
chemical_list: D004275:DNA, Ribosomal; D052979:Phycobiliproteins