Molecular phylogenetic analysis of HIV-1 vif gene from Korean isolates.
Phylogenetic studies of nef, pol, and env gene sequences of HIV-1 isolated from Koreans suggested the presence of a Korean clade in which Korean sequences are clustered to the exclusion of foreign sequences. We attempted to identify and characterize the Korean clade using all vif gene sequences isolated from Koreans registered in the NCBI GenBank database (n = 233). Most (77 %) of the Korean isolates belonged to the Korean clade as a large subcluster in subtype B, designated the Korean clade subtype B (KCB). KCB sequences were relatively homogenous compared to Korean subtype B sequences that did not belong to the KCB (non-Korean clade subtype B; NKCB). Comparison of amino acid frequencies of KCB and NKCB sequences revealed several positions where the amino acid frequencies were significantly different. These amino acid residues were critical in separating KCB from NKCB or from foreign sequences, since substitution of these amino acids in KCB with the NKCB amino acids relocated the KCB sequences to NKCB, and vice versa. Further analyses of KCB will help us to understand the origin and evolutionary history of KCB.
Park
Chan Seung
CS
School of Life Sciences, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea.
Kim
Mi Sook
MS
Lee
Sung Duk
SD
Kim
Sung Soo
SS
Lee
Keon Myung
KM
Lee
Chan-Hee
CH
D000595:Amino Acid Sequence; D001483:Base Sequence; D019143:Evolution, Molecular; D016346:Gene Products, vif / Q000235:genetics*; D016341:Genes, vif; D015658:HIV Infections / Q000821:virology; D015497:HIV-1 / Q000145:classification* / Q000235:genetics; D006801:Humans; D007723:Korea; D010802:Phylogeny; D016415:Sequence Alignment; D054320:vif Gene Products, Human Immunodeficiency Virus
D016346:Gene Products, vif; D054320:vif Gene Products, Human Immunodeficiency Virus