title: Immediate activation fails to rescue efficient human immunodeficiency virus replication in quiescent CD4+ T cells.
abstract: Unlike activated T cells, quiescent CD4+ T cells have shown resistance to human immunodeficiency virus (HIV) infection due to a block in the early events of the viral life cycle. To further investigate the nature of this block, we infected quiescent CD4+ T cells with HIV-1(NL4-3) and immediately stimulated them. Compared to activated (prestimulated) cells, these poststimulated cells showed slightly decreased viral entry and delays in the completion of reverse transcription. However, the relative efficiency of integration was similar to that of prestimulated cells. Together, this resulted in decreased expression of tat/rev mRNA and synthesis of viral protein. Furthermore, based on cell cycle staining and BrdU incorporation, poststimulated cells expressing viral protein failed to initiate a second round of their cell cycle, independently of Vpr-mediated arrest. Together, these data demonstrate that the early stages of the HIV life cycle are inefficient in these poststimulated cells and that efficient replication cannot be induced by subsequent activation.
authors.affiliation: David Geffen School of Medicine at UCLA, 650 Charles Young Dr. South, Box 951678, 11-934 Factor, Los Angeles, CA 90095-1678, USA.
authors.forename: Dimitrios N
authors.initials: DN
authors.lastname: Vatakis
authors.forename: Gregory
authors.initials: G
authors.lastname: Bristol
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Wilkinson
authors.forename: Samson A
authors.initials: SA
authors.lastname: Chow
authors.forename: Jerome A
authors.initials: JA
authors.lastname: Zack
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002460:Cell Line; D049109:Cell Proliferation; D015967:Gene Expression Regulation, Viral; D015683:Gene Products, gag / Q000378:metabolism; D016336:Gene Products, vpr / Q000378:metabolism; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D008213:Lymphocyte Activation / Q000276:immunology*; D012367:RNA, Viral / Q000235:genetics; D048348:Reverse Transcription / Q000235:genetics; D053586:Virus Internalization; D014779:Virus Replication; D054325:vpr Gene Products, Human Immunodeficiency Virus
chemical_list: D015683:Gene Products, gag; D016336:Gene Products, vpr; D012367:RNA, Viral; D054325:vpr Gene Products, Human Immunodeficiency Virus