title: Quantitative assessment of the tetracycline resistance gene pool in cheese samples by real-time TaqMan PCR.
abstract: A TaqMan real-time PCR assay was developed to quantify the tetS gene pool present in retail cheeses. This protocol offers a rapid, specific, sensitive, and culture-independent method for assessing antibiotic resistance genes in food samples rich in fats and proteins.
authors.affiliation: Department of Food Science and Technology, The Ohio State University, Parker FST Building, 2015 Fyffe Ct., Columbus, OH 43210-1007, USA.
authors.forename: Michele Y
authors.initials: MY
authors.lastname: Manuzon
authors.forename: Scott E
authors.initials: SE
authors.lastname: Hanna
authors.forename: Hongliang
authors.initials: H
authors.lastname: Luo
authors.forename: Zhongtang
authors.initials: Z
authors.lastname: Yu
authors.forename: W James
authors.initials: WJ
authors.lastname: Harper
authors.forename: Hua H
authors.initials: HH
authors.lastname: Wang
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D002611:Cheese / Q000382:microbiology*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis* / Q000302:isolation & purification; D026901:Membrane Transport Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D019914:Taq Polymerase; D013753:Tetracycline Resistance / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D026901:Membrane Transport Proteins; D019914:Taq Polymerase