title: Bone marrow stromal cells stimulate neurite outgrowth over neural proteoglycans (CSPG), myelin associated glycoprotein and Nogo-A.
abstract: In animal models, transplantation of bone marrow stromal cells (MSC) into the spinal cord following injury enhances axonal regeneration and promotes functional recovery. How these improvements come about is currently unclear. We have examined the interaction of MSC with neurons, using an established in vitro model of nerve growth, in the presence of substrate-bound extracellular molecules that are thought to inhibit axonal regeneration, i.e., neural proteoglycans (CSPG), myelin associated glycoprotein (MAG) and Nogo-A. Each of these molecules repelled neurite outgrowth from dorsal root ganglia (DRG) in a concentration-dependent manner. However, these nerve-inhibitory effects were much reduced in MSC/DRG co-cultures. Video microscopy demonstrated that MSC acted as "cellular bridges" and also "towed" neurites over the nerve-inhibitory substrates. Whereas conditioned medium from MSC cultures stimulated DRG neurite outgrowth over type I collagen, it did not promote outgrowth over CSPG, MAG or Nogo-A. These findings suggest that MSC transplantation may promote axonal regeneration both by stimulating nerve growth via secreted factors and also by reducing the nerve-inhibitory effects of the extracellular molecules present.
authors.affiliation: Centre for Spinal Studies, Robert Jones & Agnes Hunt Orthopaedic Hospital, Oswestry, Shropshire SY10 7AG, UK.
authors.forename: Karina T
authors.initials: KT
authors.lastname: Wright
authors.forename: Wagih
authors.initials: W
authors.lastname: El Masri
authors.forename: Aheed
authors.initials: A
authors.lastname: Osman
authors.forename: Sally
authors.initials: S
authors.lastname: Roberts
authors.forename: Giselle
authors.initials: G
authors.lastname: Chamberlain
authors.forename: Brian A
authors.initials: BA
authors.lastname: Ashton
authors.forename: William E B
authors.initials: WE
authors.lastname: Johnson
mesh_terms: D000328:Adult; D000368:Aged; D001854:Bone Marrow Cells / Q000502:physiology*; D049108:Cell Enlargement; D002478:Cells, Cultured; D006801:Humans; D008875:Middle Aged; D009185:Myelin Proteins / Q000378:metabolism*; D018992:Myelin-Associated Glycoprotein / Q000378:metabolism*; D016501:Neurites / Q000502:physiology*; D009474:Neurons / Q000166:cytology* / Q000502:physiology; D000070798:Nogo Proteins; D011509:Proteoglycans / Q000378:metabolism*; D017154:Stromal Cells / Q000502:physiology
chemical_list: D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D000070798:Nogo Proteins; D011509:Proteoglycans; C000603199:RTN4 protein, human