Extracellular amino acid levels in the human liver during transplantation: a microdialysis study from donor to recipient.
Using microdialysis, we have monitored extracellular levels of amino acids and related amines in the human liver at three stages of the transplantation procedure: donor retrieval, back table preparation and during 48 h post-implantation. By comparing the ratio of mean levels at the donor and back table stages, with the ratio between early (2-6 h) and late (43-48 h) post-reperfusion, these amines were classified into one of three groups. In one group, back table levels were markedly higher than during the donor stage, with levels declining over time post-reperfusion. A second group had much lower levels in the back table than during the donor phase, and post-reperfusion levels were either stable or increased over time. Concentrations of amino acids in the final group remained relatively constant at all stages. This study illustrates the value of microdialysis in providing organ-specific metabolic data that may indicate specific mechanisms of poor graft function.
Richards
D A
DA
Department of Pharmacology, Medical School, University of Birmingham, Edgbaston, Birmingham, UK. d.a.richards@bham.ac.uk
Silva
M A
MA
Murphy
N
N
Wigmore
S J
SJ
Mirza
D F
DF
D000293:Adolescent; D000328:Adult; D000368:Aged; D000596:Amino Acids / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000737:chemistry* / Q000378:metabolism; D016031:Liver Transplantation; D008297:Male; D017551:Microdialysis; D008875:Middle Aged; D014019:Tissue Donors
D000596:Amino Acids