Determination of hydroxysafflor yellow A in rat plasma and tissues by high-performance liquid chromatography after oral administration of safflower extract or safflor yellow.
A simple and reproducible HPLC method for quantification of hydroxysafflor yellow A (HSYA) in rat plasma and tissues after oral administration of safflower extract or safflor yellow (SY) was developed. Sample preparation was achieved by protein precipitation of plasma and tissue homogenates with three volumes of methanol. p-Hydroxybenzaldehyde was used as the internal standard (IS). HSYA and IS were separated on a Hypersil BDS-C(18) column with a gradient elution system composed of acetonitrile and aqueous acetic acid. UV detection was used at 320 nm. The calibration curves were linear in all matrices (r(2) > 0.999) in the concentration ranges 0.51-101.36 microg/mL for plasma, 12.27-2454.46 microg/g for intestines and 0.96-192.20 microg/g for lung. The intra-day and inter-day precision were all less than 12.5%, and the extract recovery was in the range 64.1-103.7% with RSD less than 15.6% for HSYA in all matrices. The method was used successfully to quantify HSYA in rat plasma and tissue samples to support a pharmacokinectic study.
Li
Yi
Y
Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, People's Republic of China.
Zhang
Zhao-Yang
ZY
Zhang
Jin-Lan
JL
D000284:Administration, Oral; D000818:Animals; D001547:Benzaldehydes; D031187:Carthamus tinctorius / Q000737:chemistry; D002599:Chalcone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007422:Intestines / Q000737:chemistry; D008168:Lung / Q000737:chemistry; D010936:Plant Extracts / Q000008:administration & dosage; D010949:Plasma / Q000737:chemistry; D011809:Quinones / Q000032:analysis*; D051381:Rats; D015203:Reproducibility of Results; D012450:Safflower Oil / Q000008:administration & dosage / Q000032:analysis*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D014018:Tissue Distribution
D001547:Benzaldehydes; D010936:Plant Extracts; D011809:Quinones; C076422:safflower yellow; C085278:hydroxysafflor yellow A; D002599:Chalcone; D012450:Safflower Oil; C011483:4-hydroxybenzaldehyde