Phenol red in the culture medium strongly affects the susceptibility of human MCF-7 cells to roscovitine.
Estrogens play an important role in the growth and terminal differentiation of the mammary gland. Prolonged exposure to estrogens seems to predispose women to breast cancer. It recently became evident that not only the intrinsic hormonal status but also external factors such as the occurrence of pharmaceuticals and chemicals with hormone activity in the environment may put women at greater risk of developing breast cancer. We focused on the interference of endocrine disruptors in breast cancer therapy. We observed that phenol red added to the culture medium strongly promoted the cell proliferation and cell cycle progression of human cells expressing the estrogen receptor, and affected their susceptibility to chemotherapy.
Wesierska-Gadek
Józefa
J
Institute of Cancer Research, Department of Medicine I, Vienna Medical University, Vienna, Austria. Jozefa.Gadek-Wesierski@meduniwien.ac.at
Schreiner
Tanja
T
Maurer
Margarita
M
Waringer
Astrid
A
Ranftler
Carmen
C
D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003470:Culture Media / Q000737:chemistry* / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology; D006801:Humans; D010637:Phenolsulfonphthalein / Q000737:chemistry / Q000494:pharmacology*; D047428:Protein Kinase Inhibitors / Q000737:chemistry / Q000494:pharmacology; D011687:Purines / Q000494:pharmacology*; D000077546:Roscovitine
D003470:Culture Media; D004967:Estrogens; D047428:Protein Kinase Inhibitors; D011687:Purines; D000077546:Roscovitine; D010637:Phenolsulfonphthalein