title: [Inhibitory effects of 11 coumarin compounds against growth of human bladder carcinoma cell line E-J in vitro].
abstract: OBJECTIVE
  To screen antitumor active compounds, drug-like or leading compounds from Chinese traditional and herbal drugs.
  
  
  METHODS
  Eleven coumarin compounds isolated from the Chinese traditional and herbal drugs were studied for their antitumor activities in vitro by determining the inhibition rates against growth of human bladder carcinoma cell line E-J.
  
  
  RESULTS
  It showed that umbelliferone, scoparone, demethylfuropinarine, isopimpinellin, forbesoside, columbianadin, decursin and glycycoumarin inhibited the growth of human bladder carcinoma cell line E-J in vitro and their activities showed a concentration-effect relationship. The inhibitory effects of forbesoside, columbianadin, decursin and umbelliferone, with IC50 values of 7.50x10(-7), 2.30x10(-6), 6.00x10(-6) and 1.30x10(-6) mol/L, respectively, were stronger than those of the other tested compounds. However, xanthotoxin, esculin and sphondin did not inhibit the growth of human bladder carcinoma cell line E-J in this assay condition.
  
  
  CONCLUSIONS
  These findings indicate that forbesoside, columbianadin, esculin, decursin and umbelliferone would be effective or regarded as potent drug-like or leading compounds against human bladder carcinoma.
authors.affiliation: The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Science, Peking University, Beijing 100083, China. wshx-001@163.com
authors.forename: Xiu-wei
authors.initials: XW
authors.lastname: Yang
authors.forename: Bo
authors.initials: B
authors.lastname: Xu
authors.forename: Fu-xiang
authors.initials: FX
authors.lastname: Ran
authors.forename: Rui-qing
authors.initials: RQ
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.forename: Jing-rong
authors.initials: JR
authors.lastname: Cui
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D001578:Benzopyrans / Q000737:chemistry / Q000494:pharmacology; D002087:Butyrates / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003374:Coumarins / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D004929:Esculin / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D015394:Molecular Structure; D014468:Umbelliferones / Q000737:chemistry / Q000494:pharmacology; D001749:Urinary Bladder Neoplasms / Q000473:pathology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D001578:Benzopyrans; D002087:Butyrates; D003374:Coumarins; D014468:Umbelliferones; D004929:Esculin; C030123:coumarin; C101278:decursin; C018145:scoparone