[The influence of ecdysteroid fraction from Serratula coronata on transmembrane ionic currents of snail neurons].
Changes of the transmembrane potassium, calcium, and sodium ion currents in isolated neurons of Lymnaea stagnalis snail under the action of ecdysteroid fraction (serpisten) from Serratula coronata L. applied from outside in 0.01 -1000 microg/ml concentrations have been studied using the voltage-clamp technique. It is established for the first time that serpisten non-selectively activates the potassium and calcium ion currents (the currents amplitude increases by 2 - 15%) and reduces nonspecific membrane leakage currents in the entire range of concentrations. The sodium ion current also increased by 4 - 7% under the action of Serpisten in low concentrations (0.01 -10 microg/ml) in comparison with control, but this current decreased by 5 -10% at serpisten concentrations of 100 and 1000 microg/ml. The effects were reversible. The kinetics of currents was not changed under the action of serpisten.
Vislobokov
A I
AI
Volodin
V V
VV
Ignatov
Iu D
IuD
Mel'nikov
K N
KN
Prosheva
V I
VI
D000818:Animals; D019659:Asteraceae / Q000737:chemistry*; D002118:Calcium / Q000378:metabolism; D002412:Cations / Q000378:metabolism; D026461:Ecdysteroids / Q000302:isolation & purification / Q000494:pharmacology*; D066298:In Vitro Techniques; D008195:Lymnaea / Q000166:cytology; D008564:Membrane Potentials / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism
D002412:Cations; D026461:Ecdysteroids; D010936:Plant Extracts; D012964:Sodium; D011188:Potassium; D002118:Calcium