Measurement of lysophospholipase D/autotaxin activity in human serum samples.
OBJECTIVE
Lysophospholipase D (lysoPLD)/autotaxin regulates the blood levels of lysophosphatidic acid (LPA), and we designed a serum lysoPLD assay for use in clinical laboratory testing.


METHODS
LysoPLD activity was assessed based on the amount of choline released with lysophosphatidylcholine as the substrate.


CONCLUSIONS
It was confirmed that the lysoPLD assay can be applied to clinical laboratory testing. The serum lysoPLD activity in healthy female subjects was significantly higher than that in the male subjects.
Nakamura
Kazuhiro
K
Department of Clinical Laboratory, The University of Tokyo Hospital, Tokyo, Japan.
Ohkawa
Ryunosuke
R
Okubo
Shigeo
S
Tozuka
Minoru
M
Okada
Mitsumasa
M
Aoki
Shinya
S
Aoki
Junken
J
Arai
Hiroyuki
H
Ikeda
Hitoshi
H
Yatomi
Yutaka
Y
D019411:Clinical Laboratory Techniques; D005260:Female; D006801:Humans; D008297:Male; D009097:Multienzyme Complexes / Q000097:blood*; D043264:Phosphodiesterase I / Q000097:blood*; D010727:Phosphoric Diester Hydrolases / Q000097:blood*; D011755:Pyrophosphatases / Q000097:blood*; D012680:Sensitivity and Specificity; D012737:Sex Factors; D000099189:Lysophospholipase D
D009097:Multienzyme Complexes; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases; D011755:Pyrophosphatases; D000099189:Lysophospholipase D