Determination of biotin by high-performance liquid chromatography in infant formula, medical nutritional products, and vitamin premixes.
A solid-phase extraction sample preparation procedure was developed for use with a high-performance liquid chromatography (HPLC) method for biotin analysis. The HPLC method used a reversed-phase C18 column; chromatography run time was 8.5 min. After eluting from the column, biotin went through postcolumn reaction to form a conjugate with streptavidin-fluorescein isothiocyanate, which was then detected by a fluorescence detector. This method was tested with infant formula, medical nutritional products, and vitamin premix samples.
Thompson
Linda B
LB
Abbott Laboratories, Ross Products Division, Research and Services, 3300 Stelzer Rd, Columbus, OH 43215, USA.
Schmitz
Daniel J
DJ
Pan
Shang-Jing
SJ
D001710:Biotin / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D019587:Dietary Supplements / Q000032:analysis*; D006801:Humans; D007202:Indicators and Reagents; D007223:Infant; D007225:Infant Food / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D012256:Riboflavin / Q000032:analysis; D012996:Solutions; D014815:Vitamins / Q000032:analysis*
D007202:Indicators and Reagents; D012996:Solutions; D014815:Vitamins; D001710:Biotin; D012256:Riboflavin