[Short communication: comparison of two different assays for the investigation of HBsAG, anti-HCV and anti-HIV in blood donors].
Acquisition of infectious agents during blood transfusions is an important complication. The choice of screening tests is of crucial importance since it is difficult to detect the markers of the infection in blood donors, especially before the window period when seroconversion has not taken place. In this study the blood samples of 150 volunteer donors were screened for anti-HIV 1/2, anti-HCV, and HBsAg by chemiluminescence method. Samples found to be positive for anti-HIV 1/2 and anti-HCV were confirmed by Western Blot and Inno-Lia HCV Ab III tests, respectively. HBsAg, anti-HCV and anti-HIV 1/2 antibodies were also investigated by the Simul-Tec immunoassay in all of the samples, and the results were compared. As a result, 4.7% of the donors were positive for HBsAg and confirmatory tests showed that the positivity rate was 2.7% for anti-HCV, 0.7% for anti-HIV 1/ 2. Although the results obtained with the Simul-Tec immunoassay were similar to the screening test results obtained for HBsAg, discrepant results were obtained for anti-HCV and anti-HIV 1/ 2. The results of this study indicated that Simul-Tec immunassay method is not yet ready to meet the demands in terms of anti-HCV and anti-HIV detection.
Cavdar
Esra
E
Kizilay Derneği Ankara Kan Merkezi, Ankara.
Kuştimur
Semra
S
Doğruman Al
Funda
F
D001782:Blood Donors; D015153:Blotting, Western; D015483:HIV Antibodies / Q000097:blood*; D015658:HIV Infections / Q000175:diagnosis; D015497:HIV-1 / Q000276:immunology*; D015498:HIV-2 / Q000276:immunology*; D006509:Hepatitis B / Q000175:diagnosis; D006514:Hepatitis B Surface Antigens / Q000097:blood*; D006526:Hepatitis C / Q000175:diagnosis; D018937:Hepatitis C Antibodies / Q000097:blood*; D006801:Humans; D007118:Immunoassay; D008163:Luminescent Measurements; D008403:Mass Screening / Q000379:methods
D015483:HIV Antibodies; D006514:Hepatitis B Surface Antigens; D018937:Hepatitis C Antibodies