Pyramiding transgenic resistance in elite indica rice cultivars against the sheath blight and bacterial blight.
Elite indica rice cultivars were cotransformed with genes expressing a rice chitinase (chi11) and a thaumatin-like protein (tlp) conferring resistance to fungal pathogens and a serine-threonine kinase (Xa21) conferring bacterial blight resistance, through particle bombardment, with a view to pyramiding sheath blight and bacterial blight resistance. Molecular analyses of putative transgenic lines by polymerase chain reaction, Southern Blot hybridization, and Western Blotting revealed stable integration and expression of the transgenes in a few independent transgenic lines. Progeny analyses showed the stable inheritance of transgenes to their progeny. Coexpression of chitinase and thaumatin-like protein in the progenies of a transgenic Pusa Basmati1 line revealed an enhanced resistance to the sheath blight pathogen, Rhizoctonia solani, as compared to that in the lines expressing the individual genes. A transgenic Pusa Basmati1 line pyramided with chi11, tlp, and Xa21 showed an enhanced resistance to both sheath blight and bacterial blight.
Maruthasalam
S
S
Rice Transformation Laboratory, Center for Plant Molecular Biology, Department of Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore 641003, Tamil Nadu, India.
Kalpana
K
K
Kumar
K K
KK
Loganathan
M
M
Poovannan
K
K
Raja
J A J
JA
Kokiladevi
E
E
Samiyappan
R
R
Sudhakar
D
D
Balasubramanian
P
P
D001483:Base Sequence; D019470:Biolistics; D015139:Blotting, Southern; D015153:Blotting, Western; D017931:DNA Primers; D012275:Oryza / Q000235:genetics* / Q000382:microbiology; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000382:microbiology; D016133:Polymerase Chain Reaction
D017931:DNA Primers