title: Effect of a desensitizing agent containing glutaraldehyde and HEMA on bond strength to Er:YAG laser-irradiated dentine.
abstract: OBJECTIVE
  The aim of this study was to investigate whether a desensitizing agent (GLUMA Desensitizer) containing glutaraldehyde and HEMA improved the bond strength and bonding durability of a self-etching primer adhesive to Er:YAG-irradiated dentine.
  
  
  METHODS
  Dentine of 120 human molars was exposed by wet grinding on SiC paper for bond strength testing. Thirty specimens each were allocated to the following treatment groups: (1) control; (2) Er:YAG laser irradiation; (3) Er:YAG laser irradiation followed by application of GLUMA Desensitizer; (4) Er:YAG laser irradiation followed by application of GLUMA Desensitizer and 10s rinsing with water. Composite cylinders were bonded to the dentine surfaces with a self-etching priming adhesive system. Tensile bond strengths (TBS) of 10 specimens of each treatment group were measured after 24-h water storage, 6 months water storage and 12 months water storage, respectively, and the failure modes were analyzed. TBS data were statistically treated by two-way ANOVA and Fisher's PLSD test at a significance level of p<0.05.
  
  
  RESULTS
  TBSs for the GLUMA-non rinse and GLUMA-rinse groups were significantly higher than for the laser group at 24 h and 12 months. Specimens from the Er:YAG-irradiated dentine group had significantly lower bond strengths than the control group at each storage time. All control specimens showed cohesive fractures in resin close to the bonding interface whereas the Er:YAG laser-irradiated groups showed both dentine cohesive, resin cohesive and dentine-resin mixed failures.
  
  
  CONCLUSIONS
  Application of GLUMA Desensitizer to Er:YAG-irradiated dentine increases the bond strength and durability of the self-etching priming adhesive used.
authors.affiliation: Department of Operative Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8525, Japan. omae@md.okayama-u.ac.jp
authors.forename: Masanori
authors.initials: M
authors.lastname: Omae
authors.forename: Masataka
authors.initials: M
authors.lastname: Inoue
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Itota
authors.forename: Werner J
authors.initials: WJ
authors.lastname: Finger
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Inoue
authors.forename: Kumiko
authors.initials: K
authors.lastname: Tanaka
authors.forename: Kazuyo
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yoshiyama
mesh_terms: D003188:Composite Resins; D001840:Dental Bonding; D045662:Dental Etching / Q000379:methods*; D003799:Dental Stress Analysis; D003807:Dentin Sensitivity / Q000188:drug therapy; D005976:Glutaral; D006801:Humans; D007834:Lasers; D008689:Methacrylates; D008963:Molar; D019279:Resin Cements; D013718:Tensile Strength
chemical_list: C438313:Clearfil SE Bond; D003188:Composite Resins; D008689:Methacrylates; D019279:Resin Cements; C111486:gluma desensitizer; D005976:Glutaral