Recombinant protein-based assays for detection of antibodies to severe acute respiratory syndrome coronavirus spike and nucleocapsid proteins.
Recombinant severe acute respiratory syndrome (SARS) nucleocapsid and spike protein-based immunoglobulin G immunoassays were developed and evaluated. Our assays demonstrated high sensitivity and specificity to the SARS coronavirus in sera collected from patients as late as 2 years postonset of symptoms. These assays will be useful not only for routine SARS coronavirus diagnostics but also for epidemiological and antibody kinetic studies.
Haynes
Lia M
LM
National Centers for Immunization and Respiratory Diseases, Division of Viral Diseases, Respiratory and Gastroenteritis Viruses Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, Mailstop G-18, Atlanta, GA 30333. loh5@cdc.gov
Miao
Congrong
C
Harcourt
Jennifer L
JL
Montgomery
Joel M
JM
Le
Mai Quynh
MQ
Dryga
Sergey A
SA
Kamrud
Kurt I
KI
Rivers
Bryan
B
Babcock
Gregory J
GJ
Oliver
Jennifer Betts
JB
Comer
James A
JA
Reynolds
Mary
M
Uyeki
Timothy M
TM
Bausch
Daniel
D
Ksiazek
Thomas
T
Thomas
William
W
Alterson
Harold
H
Smith
Jonathan
J
Ambrosino
Donna M
DM
Anderson
Larry J
LJ
D000914:Antibodies, Viral / Q000097:blood*; D000086462:Coronavirus Nucleocapsid Proteins; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008562:Membrane Glycoproteins / Q000276:immunology*; D019590:Nucleocapsid Proteins / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology*; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D045169:Severe Acute Respiratory Syndrome / Q000175:diagnosis*; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D000086462:Coronavirus Nucleocapsid Proteins; D008562:Membrane Glycoproteins; D019590:Nucleocapsid Proteins; D011994:Recombinant Proteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; C578557:spike glycoprotein, SARS-CoV