Proteomic analysis of the carbonate insoluble outer membrane fraction of the soft-rot pathogen Dickeya dadantii (syn. Erwinia chrysanthemi) strain 3937.
We present results of the first comprehensive proteomic analysis of the outer membrane of the bacterial phytopathogen Dickeya dadantii strain 3937 and its response to virulence-contributing factors such as host plant extract, acidic stress, and iron starvation. We analyzed the carbonate-insoluble membrane fractions, which are highly enriched for outer membrane proteins, using two-dimensional electrophoresis and identified the proteins by MALDI-TOF MS. Forty unique proteins were identified, some of which were differentially expressed under the above conditions.
Babujee
Lavanya
L
Laboratory of Plant Pathology, Faculty of Agriculture, Shizuoka University, Shizuoka 422-8529, Japan.
Venkatesh
Balakrishnan
B
Yamazaki
Akihiro
A
Tsuyumu
Shinji
S
D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D002254:Carbonates / Q000737:chemistry*; D019295:Computational Biology / Q000379:methods*; D016974:Dickeya chrysanthemi / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D006023:Glycoproteins / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008074:Lipoproteins / Q000737:chemistry; D010766:Phosphorylation; D010944:Plants / Q000382:microbiology*; D011499:Protein Processing, Post-Translational; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014774:Virulence
D001425:Bacterial Outer Membrane Proteins; D002254:Carbonates; D006023:Glycoproteins; D008074:Lipoproteins; D020543:Proteome