An annotated cDNA library and microarray for large-scale gene-expression studies in the ant Solenopsis invicta.
Ants display a range of fascinating behaviors, a remarkable level of intra-species phenotypic plasticity and many other interesting characteristics. Here we present a new tool to study the molecular mechanisms underlying these traits: a tentatively annotated expressed sequence tag (EST) resource for the fire ant Solenopsis invicta. From a normalized cDNA library we obtained 21,715 ESTs, which represent 11,864 putatively different transcripts with very diverse molecular functions. All ESTs were used to construct a cDNA microarray.
Wang
John
J
Department of Ecology and Evolution, University of Lausanne, 1015 Lausanne, Switzerland. John.Wang@unil.ch
Jemielity
Stephanie
S
Uva
Paolo
P
Wurm
Yannick
Y
Gräff
Johannes
J
Keller
Laurent
L
D000818:Animals; D001000:Ants / Q000235:genetics*; D001483:Base Sequence; D001522:Behavior, Animal; D002999:Clone Cells; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015723:Gene Library; D017344:Genes, Insect; D008136:Longevity / Q000235:genetics; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D012367:RNA, Viral