Expression of CD55 and CD59 on peripheral blood cells in patients with lymphoproliferative disease of granular lymphocytes.
Lymphoproliferative disease of granular lymphocytes (LDGL) is a disorder characterized by the clonal expansion of granular lymphocytes. It has recently been shown that the clonal expansion of granular lymphocytes occurs in patients with paroxysmal nocturnal hemoglobinuria (PNH) in a subclinical fashion. To test the possibility that LDGL patients share a PNH phenotype, we obtained peripheral blood cells from 20 patients with LDGL and examined the expression of the glycosylphosphatidyl inositol (GPI)-anchored proteins, CD55 and CD59. Compared with normal controls, however, a defective expression of CD55/59 was not observed on either granulocytes or erythrocytes from LDGL patients. An unexpected finding was the significantly lower CD55/59 expression on granular lymphocytes from patients with CD16(+)CD56(-) phenotype LDGL than from patients with CD16(+)CD56(+) phenotype LDGL, or natural killer (NK) and NK/T lymphocytes from healthy individuals. The positive correlation between the expression of CD56 and CD55/59 might have some relevance to the functional properties of the CD56(+) subset of large granular lymphocytes.
Isoda
A
A
Department of Medicine and Clinical Science, Graduate School of Medicine, Gunma University, Maebashi, Gunma, Japan. aisoda@netnngh.hosp.go.jp
Tsukamoto
N
N
Mitsui
T
T
Yamane
A
A
Hatsumi
N
N
Matsushima
T
T
Murakami
H
H
Nojima
Y
Y
Karasawa
M
M
D018958:CD55 Antigens / Q000096:biosynthesis*; D018957:CD59 Antigens / Q000096:biosynthesis*; D005260:Female; D005786:Gene Expression Regulation; D006457:Hemoglobinuria, Paroxysmal / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007694:Killer Cells, Natural / Q000378:metabolism* / Q000473:pathology; D008232:Lymphoproliferative Disorders / Q000378:metabolism* / Q000473:pathology; D008297:Male; D013601:T-Lymphocytes / Q000378:metabolism* / Q000473:pathology
D018958:CD55 Antigens; D018957:CD59 Antigens; C051590:CD59 protein, human