Disinfection of Legionella pneumophila by photocatalytic oxidation.
Photocatalytic oxidation (PCO) was proven to be efficacious in the inactivation of Legionella pneumophila serogroup 1 Strains 977, 1009, 1014 and ATCC 33153. The local (Strains 997, 1009 and 1014) and ATCC (Strain 33153) strains showed sensitivity differences towards PCO. The inactivation mechanisms of PCO were investigated by transmission and scanning electron microscopy by which PCO was found to disintegrate the cells eventually. Before the disintegration, there was lipid peroxidation of outer and cytoplasmic membrane causing holes formation and leading to the entry of OH into the cells to oxidize the intracellular components. Fatty acid profile analysis found that the amount of saturated, 16-carbon branched-chain fatty acid, which is predominant in Legionella, decreased in the surviving populations from PCO. A relationship between the amount of this fatty acid and the PCO sensitivity of the tested strains was also observed. Mineralization of cells by PCO was proven by total organic carbon analysis.
Cheng
Y W
YW
Department of Biology, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR, China.
Chan
Raphael C Y
RC
Wong
P K
PK
D000942:Antigens, Bacterial / Q000378:metabolism; D002384:Catalysis; D002462:Cell Membrane / Q000378:metabolism; D004203:Disinfection / Q000295:instrumentation / Q000379:methods*; D005227:Fatty Acids / Q000032:analysis; D016952:Legionella pneumophila / Q000145:classification / Q000254:growth & development / Q000378:metabolism; D007877:Legionnaires' Disease / Q000517:prevention & control*; D015227:Lipid Peroxidation; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D010084:Oxidation-Reduction; D010782:Photolysis
D000942:Antigens, Bacterial; D005227:Fatty Acids