Quantification of pseudoephedrine in human plasma by LC-MS/MS using mosapride as internal standard.
A simple, sensitive and rapid high-performance liquid chromatography/positive ion electrospray tandem mass spectrometry (LC-MS/MS) method was developed and validated for the quantification of pseudoephedrine in human plasma using mosapride as internal standard. Following solid-phase extraction, the analytes were separated using an isocratic mobile phase on a reverse-phase column and analyzed by MS/MS in the multiple-reaction monitoring mode using the respective [M + H](+) ions, m/z 166/148 for pseuoephedrine and m/z 422/198 for the IS. The method exhibited a linear dynamic range of 2-1000 ng/mL pseudoephedrine in human plasma. The lower limit of quantification was 2 ng/mL with a relative standard deviation of less than 9% for pseudoephedrine. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. The total chromatographic run time of 2 min for each sample made it possible to analyze more than 400 human plasma samples per day. The validated method has been successfully used to analyze human plasma samples for application in pharmacokinetic, bioavailability or bioequivalence studies.
Nirogi
Ramakrishna V S
RV
Biopharmaceutical Research, Suven Life Sciences Ltd, Serene Chambers, Road 7, Banjara Hills, Hyderabad 500034, India. ramakrishna_nirogi@yahoo.co.in
Kandikere
Vishwottam N
VN
Shukla
Manoj
M
Mudigonda
Koteshwara
K
Maurya
Santosh
S
Komarneni
Prashanth
P
D001549:Benzamides / Q000737:chemistry*; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D004809:Ephedrine / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D015394:Molecular Structure; D009025:Morpholines / Q000737:chemistry*; D010949:Plasma / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D001549:Benzamides; D009025:Morpholines; D004809:Ephedrine; C062720:mosapride