title: Ciliary calcium signaling is modulated by kidney injury molecule-1 (Kim1).
abstract: Primary cilia have been shown to play an important role in embryonic development as well as in postnatal life. Dysfunctional cilia are associated with situs inversus, retinal abnormalities, impaired mucociliary clearance, infertility, hydrocephalus, and congenital renal cysts. In autosomal dominant polycystic kidney disease, mutations of the ciliary proteins polycystin1 or the transient receptor potential (TRP) channel family protein polycystin2 (TRPP2) cause progressive cyst formation and destruction of the kidney. Primary cilia act as flow sensors and respond to flow-mediated bending with a prolonged intracellular calcium increase, which appears to require an intact polycystin protein complex. We have established a novel flow chamber system, which allows us to study renal epithelial cells by live cell imaging. We show that MDCK cells respond to flow by a delayed increase in intracellular calcium and that this response requires these cells to be ciliated. We show that a novel interactor of TRPP2, kidney injury molecule-1 (Kim1), which is expressed at low levels in the normal kidney and upregulated after ischemia, in renal cell cancer and in PKD is targeted to primary cilia when stably expressed in MDCK cells. We demonstrate that expression of tyrosine mutant Kim1, lacking a conserved tyrosine in the intracellular tail, abolishes the calcium increase in response to flow in a dominant negative manner. These results establish Kim1 as a novel regulatory molecule of flow-induced calcium signaling.
authors.affiliation: Department of Medicine, University Hospital Freiburg, Renal Unit, Hugstetter Str. 55, Freiburg, Germany.
authors.forename: Fruzsina
authors.initials: F
authors.lastname: Kotsis
authors.forename: Roland
authors.initials: R
authors.lastname: Nitschke
authors.forename: Christopher
authors.initials: C
authors.lastname: Boehlke
authors.forename: Mikhail
authors.initials: M
authors.lastname: Bashkurov
authors.forename: Gerd
authors.initials: G
authors.lastname: Walz
authors.forename: E Wolfgang
authors.initials: EW
authors.lastname: Kuehn
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000502:physiology*; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology; D002923:Cilia / Q000378:metabolism*; D004285:Dogs; D005456:Fluorescent Dyes; D016257:Fura-2; D000072596:Hepatitis A Virus Cellular Receptor 1; D006801:Humans; D007684:Kidney Tubules / Q000166:cytology*; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D011493:Protein Kinase C / Q000737:chemistry / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011991:Receptors, Virus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013314:Stress, Mechanical; D050396:TRPP Cation Channels / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; C491143:HAVCR1 protein, human; D000072596:Hepatitis A Virus Cellular Receptor 1; D008562:Membrane Glycoproteins; D011991:Receptors, Virus; D050396:TRPP Cation Channels; D011493:Protein Kinase C; D002118:Calcium; D016257:Fura-2