Direct detection method of oligosaccharides by high-performance liquid chromatography with charged aerosol detection.
A simple and rapid detection method of oligosaccharides using high-performance liquid chromatography with a charged aerosol detection (HPLC-CAD) was studied. The direct detection of a sialylglycopeptide (SGP) derived from egg yolk was accomplished by HPLC-CAD using an amido-silica column, and its limit of detection was 0.40 pmol [signal-to-noise ratio (S/N) = 3]. The sensitivity of this method was lower than that of the fluorescence detection; however, the method showed approximately 5 times higher sensitivity than that using the conventional UV absorbance detection. Furthermore, this method was used for the analysis of the acid hydrolysis products of SGP. Monosialo- and asialo-oligosaccharides as well as free sialic acid were detected without using fluorescent derivatization. These results indicate that the present method is a new tool for the analysis of oligosaccharides.
Inagaki
Shinsuke
S
Division of Bio-Analytical Chemistry, School of Pharmaceutical Sciences, COE Program in the 21st Century, University of Shizuoka, 52-1 Yada, Surugaku, Shizuoka 422-8526, Japan.
Min
Jun Zhe
JZ
Toyo'oka
Toshimasa
T
D000336:Aerosols / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006868:Hydrolysis; D009844:Oligosaccharides / Q000032:analysis*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D000336:Aerosols; D009844:Oligosaccharides