title: Characterization of the SP11/SCR high-affinity binding site involved in self/nonself recognition in brassica self-incompatibility.
abstract: In Brassica self-incompatibility, the recognition of self/nonself pollen grains, is controlled by the S-locus, which encodes three highly polymorphic proteins: S-locus receptor kinase (SRK), S-locus protein 11 (SP11; also designated S-locus Cys-rich protein), and S-locus glycoprotein (SLG). SP11, located in the pollen coat, determines pollen S-haplotype specificity, whereas SRK, located on the plasma membrane of stigmatic papilla cells, determines stigmatic S-haplotype specificity. SLG shares significant sequence similarity with the extracellular domain of SRK and is abundant in the stigmatic cell wall, but its function is controversial. We previously showed that SP11 binds directly to its cognate SRK with high affinity (K(d) = 0.7 nM) and induces its autophosphorylation. We also found that an SLG-like, 60-kD protein on the stigmatic membrane forms a high-affinity binding site for SP11. Here, we show that the 60-kD stigmatic membrane protein is a truncated form of SRK containing the extracellular domain, transmembrane domain, and part of the juxtamembrane domain. A transiently expressed, membrane-anchored form of SRK exhibits high-affinity binding to SP11, whereas the soluble SRK (eSRK) lacking the transmembrane domain exhibits no high-affinity binding, as is the case with SLG. The different binding affinities of the membrane-anchored SRK and soluble eSRK or SLG will be significant for the specific perception of SP11 by SRK.
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma 630-0192, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Shimosato
authors.forename: Naohiko
authors.initials: N
authors.lastname: Yokota
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shiba
authors.forename: Megumi
authors.initials: M
authors.lastname: Iwano
authors.forename: Tetsuyuki
authors.initials: T
authors.lastname: Entani
authors.forename: Fang-Sik
authors.initials: FS
authors.lastname: Che
authors.forename: Masao
authors.initials: M
authors.lastname: Watanabe
authors.forename: Akira
authors.initials: A
authors.lastname: Isogai
authors.forename: Seiji
authors.initials: S
authors.lastname: Takayama
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D001937:Brassica / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D019281:Dimerization; D006239:Haplotypes; D008861:Microsomes / Q000378:metabolism; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D011494:Protein Kinases / Q000737:chemistry / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D017434:Protein Structure, Tertiary; D012098:Reproduction / Q000235:genetics / Q000502:physiology; D016415:Sequence Alignment
chemical_list: D010940:Plant Proteins; C431526:SP11 protein, Brassica; D011494:Protein Kinases; C079832:S-receptor kinase