Antifungal activities of azole agents against the Malassezia species.
In this paper, we identified 95 Malassezia isolates by morphological and biochemical criteria and assessed the in vitro activity of fluconazole, itraconazole, ketoconazole and voriconazole by broth microdilution against these species using slightly modified Leeming-Notman medium. The Malassezia isolates were identified as M. furfur (74), M. sympodialis (11), M. obtusa (8) and M. globosa (2). The modified Leeming-Notman medium used for susceptibility testing allowed good growth of Malassezia spp. Visual reading of the minimal inhibitory concentration (MIC) was readily achieved until Day 5 of incubation at 32 degrees C. Although high MIC values of 16 microg/mL for fluconazole were observed in 9.5% of Malassezia isolates, in general these microorganisms were susceptible to all drugs studied. Interestingly, one M. globosa isolate showed high MIC values for voriconazole, itraconazole and fluconazole. For the 95 strains, the MIC ranges were <0.03-4 microg/mL for ketoconazole, <0.03 to >16 microg/mL for voriconazole, <0.125 to >64 microg/mL for fluconazole and <0.03-16 microg/mL for itraconazole. In summary, the good reproducibility and visual readings obtained using modified Leeming-Notman medium suggest that this medium should be proposed for antifungal testing of drugs against Malassezia spp.
Miranda
Karla Carvalho
KC
Laboratório de Micologia da Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
de Araujo
Crystiane Rodrigues
CR
Costa
Carolina Rodrigues
CR
Passos
Xisto Sena
XS
de Fátima Lisboa Fernandes
Orionalda
O
do Rosário Rodrigues Silva
Maria
M
D000935:Antifungal Agents / Q000494:pharmacology*; D001393:Azoles / Q000494:pharmacology*; D015725:Fluconazole / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D017964:Itraconazole / Q000494:pharmacology; D007654:Ketoconazole / Q000494:pharmacology; D008290:Malassezia / Q000187:drug effects* / Q000302:isolation & purification / Q000472:pathogenicity; D008826:Microbial Sensitivity Tests; D011743:Pyrimidines / Q000494:pharmacology; D014010:Tinea Versicolor / Q000188:drug therapy / Q000382:microbiology; D014230:Triazoles / Q000494:pharmacology; D065819:Voriconazole
D000935:Antifungal Agents; D001393:Azoles; D011743:Pyrimidines; D014230:Triazoles; D017964:Itraconazole; D015725:Fluconazole; D065819:Voriconazole; D007654:Ketoconazole