title: Hedistin: A novel antimicrobial peptide containing bromotryptophan constitutively expressed in the NK cells-like of the marine annelid, Nereis diversicolor.
abstract: A novel antimicrobial peptide, named hedistin was identified from the coelomocytes of Nereis diversicolor. Hedistin shows no obvious similarities with other known peptides and constitutes the first antimicrobial peptide containing bromotryptophans demonstrated in annelids. cDNA and mass spectrometry analysis revealed that, upon bacteria challenge, this peptide is secreted following processing of a precursor containing a signal peptide and prosequences. Hedistin was shown to possess an activity against a large spectrum of bacteria including the methicillin resistant Staphylococcus aureus and Vibrio alginolyticus. The gene was demonstrated to be constitutively and exclusively expressed in circulating NK cells like known to play an important role in the immunity of the sand worm. These data contrast with those observed in another annelid, the leech, in which genes coding for antimicrobial peptides are upregulated in a specific tissue and peptides are rapidly released into the hemolymph after septic injury.
authors.affiliation: Laboratoire de Neuroimmunologie des Annélides CNRS FRE 2933, SN3 1er étage, USTL 59655 Villeneuve d'Ascq, France. aurelie.tasiemski@univ-lillel.fr
authors.forename: Aurélie
authors.initials: A
authors.lastname: Tasiemski
authors.forename: David
authors.initials: D
authors.lastname: Schikorski
authors.forename: Françoise
authors.initials: F
authors.lastname: Le Marrec-Croq
authors.forename: Christelle
authors.initials: C
authors.lastname: Pontoire-Van Camp
authors.forename: Céline
authors.initials: C
authors.lastname: Boidin-Wichlacz
authors.forename: Pierre-Eric
authors.initials: PE
authors.lastname: Sautière
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D001483:Base Sequence; D015152:Blotting, Northern; D018076:DNA, Complementary; D015870:Gene Expression; D006098:Granulocytes; D017403:In Situ Hybridization; D007694:Killer Cells, Natural / Q000737:chemistry* / Q000276:immunology; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D010455:Peptides / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D011077:Polychaeta / Q000235:genetics / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000890:Anti-Infective Agents; D018076:DNA, Complementary; D010455:Peptides