title: Proteomic analysis of the carbonate insoluble outer membrane fraction of the soft-rot pathogen Dickeya dadantii (syn. Erwinia chrysanthemi) strain 3937.
abstract: We present results of the first comprehensive proteomic analysis of the outer membrane of the bacterial phytopathogen Dickeya dadantii strain 3937 and its response to virulence-contributing factors such as host plant extract, acidic stress, and iron starvation. We analyzed the carbonate-insoluble membrane fractions, which are highly enriched for outer membrane proteins, using two-dimensional electrophoresis and identified the proteins by MALDI-TOF MS. Forty unique proteins were identified, some of which were differentially expressed under the above conditions.
authors.affiliation: Laboratory of Plant Pathology, Faculty of Agriculture, Shizuoka University, Shizuoka 422-8529, Japan.
authors.forename: Lavanya
authors.initials: L
authors.lastname: Babujee
authors.forename: Balakrishnan
authors.initials: B
authors.lastname: Venkatesh
authors.forename: Akihiro
authors.initials: A
authors.lastname: Yamazaki
authors.forename: Shinji
authors.initials: S
authors.lastname: Tsuyumu
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D002254:Carbonates / Q000737:chemistry*; D019295:Computational Biology / Q000379:methods*; D016974:Dickeya chrysanthemi / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D006023:Glycoproteins / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008074:Lipoproteins / Q000737:chemistry; D010766:Phosphorylation; D010944:Plants / Q000382:microbiology*; D011499:Protein Processing, Post-Translational; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014774:Virulence
chemical_list: D001425:Bacterial Outer Membrane Proteins; D002254:Carbonates; D006023:Glycoproteins; D008074:Lipoproteins; D020543:Proteome