title: Tracking of human Y receptors in living cells--a fluorescence approach.
abstract: Non-invasive methods for studying biological processes in living cells have become very important, also in the field of GPCR biochemistry. Great advancements in the application of fluorescence techniques as well as in the development and improvement of novel fluorophores allow the visualization of dynamic processes. Using these technologies, problems concerning receptor biosynthesis, internalization, recycling and degradation can be investigated. Here we compare the application of the different fluorescent tags EYFP, Lumiotrade mark and SNAPtrade mark to track hY(1) and hY(5) receptors in living cells.
authors.affiliation: Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, University of Leipzig, Brüderstr. 34, D-04103 Leipzig, Germany.
authors.forename: Ilka
authors.initials: I
authors.lastname: Böhme
authors.forename: Karin
authors.initials: K
authors.lastname: Mörl
authors.forename: Darja
authors.initials: D
authors.lastname: Bamming
authors.forename: Cindy
authors.initials: C
authors.lastname: Meyer
authors.forename: Annette G
authors.initials: AG
authors.lastname: Beck-Sickinger
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D006801:Humans; D008856:Microscopy, Fluorescence; D017476:Receptors, Neuropeptide Y / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D017931:DNA Primers; D017476:Receptors, Neuropeptide Y