Serodiagnostic comparison between two methods, ELISA and surface plasmon resonance for the detection of antibodies of classical swine fever.
A protein chip based on surface plasmon resonance (SPR) was developed to measure the antibody (Ab) titers of classical swine fever virus (CSFV) using the recombinant gp55 protein as an antigen. The diagnostic potential of this SPR assay for detecting the Ab titers to CSFV gp55 was compared that of the enzyme-linked immunosorbent assay (ELISA) using 170 serum samples from 14 pig farms. The SPR assay was highly specific and sensitive, and there were no cross-reactions detected. There was a strong positive correlation between the SPR and ELISA titers (n=170, r=0.869, p<0.01). Therefore, the SPR label-free method is a valuable tool in the serodiagnosis of CSFV infection and determining Ab titers after vaccination.
Cho
Ho-Seong
HS
Department of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University, Gwangju, Korea.
Park
Nam-Yong
NY
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D006691:Classical Swine Fever / Q000097:blood* / Q000175:diagnosis / Q000276:immunology; D006692:Classical Swine Fever Virus / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D020349:Surface Plasmon Resonance / Q000662:veterinary*; D013552:Swine
D000914:Antibodies, Viral