title: Comparison of polarimetric retinal nerve fiber analyzer parameters for a single high quality image and the mean image from three high quality images.
abstract: BACKGROUND
  To find the correlations and differences between the nerve fiber layer parameters of a GDx (polarimetric retinal nerve fiber analyzer) in single high quality images and those in the mean image from three high quality images.
  
  
  METHODS
  The results of scanning laser polarimetry were selected from 63 eyes of 63 patients (31 male and 32 female). Complete ophthalmic examinations included slit-lamp biomicroscopy, dilated pupil for vitreous and ocular fundus examination, applanation tonometry, and subjective or objective refraction. There were no significant ophthalmic disorders other than glaucoma and mild lens opacity. Nerve fiber layer thickness of each eye was measured with GDx and at least three high quality images (passing the software's quality criteria) were obtained. The best of these three high quality images was selected as a single high quality image (Group One). The mean image (Group Two) was calculated from the same three high quality images.
  
  
  RESULTS
  We found that there was a high correlation in all nerve fiber layer parameters between Group One and Group Two. There was no significant difference in the seven relative values of nerve fiber layer parameters (Number, Ellipse Modulation, Symmetry, Superior Ratio, Inferior Ratio, Superior/Nasal and Maximal Modulation) between Group One and Group Two. Group One had significantly higher values compared with Group Two in the other seven absolute values of nerve fiber layer parameters (Average Thickness, Ellipse Average, Superior Average, Inferior Average, Superior Integral, Superior Maximal and Inferior Maximal).
  
  
  CONCLUSIONS
  It is reasonable to take a mean image from three good quality images from a cooperative patient. If only one high quality image can be obtained in repeated acquisition of GDx, the seven relative values of nerve fiber layer parameters in this high quality image can be used as a base-line image for detecting retinal nerve fiber layer defects and for determining changes of retinal nerve fiber layer thickness in sequential images of GDx.
authors.affiliation: Department of Ophthalmology, Chang Gung Memorial Hospital. 123, Dabi Road, Niaosung Shiang, Kaohsiung, Taiwan 833, ROC. e12014@cgmh.org.tw
authors.forename: Ing-Chou
authors.initials: IC
authors.lastname: Lai
authors.forename: Ming-Tse
authors.initials: MT
authors.lastname: Kuo
authors.forename: Pei-Wen
authors.initials: PW
authors.lastname: Lin
authors.forename: Mei-Ching
authors.initials: MC
authors.lastname: Teng
authors.forename: Jen-Chia
authors.initials: JC
authors.lastname: Tsai
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003941:Diagnostic Techniques, Ophthalmological / Q000295:instrumentation*; D005260:Female; D005901:Glaucoma / Q000175:diagnosis* / Q000473:pathology; D006801:Humans; D007834:Lasers; D008297:Male; D008875:Middle Aged; D009412:Nerve Fibers / Q000473:pathology*; D009900:Optic Nerve / Q000473:pathology*; D012165:Retinal Ganglion Cells / Q000473:pathology*