title: A single residue change in Vibrio harveyi hemolysin results in the loss of phospholipase and hemolytic activities and pathogenicity for turbot (Scophthalmus maximus).
abstract: Vibrio harveyi hemolysin, an important virulence determinant in fish pathogenesis, was further characterized, and the enzyme was identified as a phospholipase B by gas chromatography. Site-directed mutagenesis revealed that a specific residue, Ser153, was critical for its enzymatic activity and for its virulence in fish.
authors.affiliation: Department of Marine Biology, Ocean University of China, 5 Yushan Road, Qingdao 266003, People's Republic of China.
authors.forename: Boguang
authors.initials: B
authors.lastname: Sun
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Zhang
authors.forename: Xuexi
authors.initials: X
authors.lastname: Tang
authors.forename: Shushan
authors.initials: S
authors.lastname: Wang
authors.forename: Yingbin
authors.initials: Y
authors.lastname: Zhong
authors.forename: Jixiang
authors.initials: J
authors.lastname: Chen
authors.forename: Brian
authors.initials: B
authors.lastname: Austin
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005393:Fish Diseases / Q000382:microbiology*; D005412:Flatfishes / Q000382:microbiology*; D006460:Hemolysin Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006461:Hemolysis; D008958:Models, Molecular; D009154:Mutation; D010740:Phospholipases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014733:Vibrio / Q000201:enzymology / Q000235:genetics / Q000472:pathogenicity*; D014735:Vibrio Infections / Q000382:microbiology / Q000662:veterinary; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D006460:Hemolysin Proteins; C073845:hemolysin, Vibrio; D010740:Phospholipases