Nicotine potentiates the nitrergic relaxation responses of rabbit corpus cavernosum tissue via nicotinic acetylcholine receptors.
The presence of neuronal nicotinic acetylcholine receptors in rabbit corpus cavernosum tissue and possible mechanisms underlying the potentiation of electrical field stimulation induced relaxation by nicotine were analyzed. In corpus cavernosum tissue strips nicotine (3 x 10(-5) M) and acetylcholine (10(-3) M) produced potentiation on electrical field stimulation (amplitude 50 V; frequency 4 Hz; width 0.8 ms) induced relaxation responses. This nicotine-induced potentiation was not altered by atropine (10(-6) M), guanethidine (5 x 10(-6) M) and indomethacin (10(-5) M), but abolished by hexamethonium chloride (10(-5) M) and L-nitro arginine methyl ester (10(-5) M). Nicotine did not cause any alteration on a single dose of carbachol (3 x 10(-5) M) and sodium nitroprusside (10(-5) M) induced relaxation responses. The results suggest that, nicotine-induced potentiation is NO and nicotinic acetylcholine receptor dependent but independent from prostaglandin synthesis, activation of muscarinic receptors and does not require intact adrenergic neurons. Nicotine did not affect smooth muscle and endothelium directly. In conclusion, in this study we showed for the first time that, nicotine acts on the nicotinic acetylcholine receptors located on the nitrergic nerves, thereby evoking the release of NO from these nerve terminals inducing relaxation response in rabbit corpus cavernosum tissue.
Bozkurt
Nihan Burul
NB
Hacettepe University, Faculty of Pharmacy, Department of Pharmacology, 06100, Sihhiye, Ankara, Turkey.
Vural
Ismail Mert
IM
Sarioglu
Yusuf
Y
Pekiner
Can
C
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects* / Q000502:physiology; D009538:Nicotine / Q000494:pharmacology*; D009569:Nitric Oxide / Q000502:physiology*; D010413:Penis / Q000187:drug effects* / Q000502:physiology; D011817:Rabbits; D011978:Receptors, Nicotinic / Q000502:physiology*
D011978:Receptors, Nicotinic; D009569:Nitric Oxide; D009538:Nicotine