title: Interaction of an organic selenium compound with human serum albumin: a spectroscopic study.
abstract: The interaction between 4,4'-diselenadibenzoic acid and human serum albumin (HSA) was investigated by fluorescence and absorption spectroscopy. The quenching mechanism of fluorescence of HSA by 4,4'-diselenadibenzoic acid was discussed. It is proved that the fluorescence quenching of HSA by 4,4'-diselenadibenzoic acid is a result of the formation of the HSA-4,4'-diselenadibenzoic acid complex. The binding sites number n, apparent corporation constant K, and corresponding thermodynamic parameters, deltaH(theta), deltaG(theta), and deltaS(theta) were calculated. Results indicate that the electrostatic interactions forces played major role in the reaction.
authors.affiliation: Department of Chemistry and Life Sciences, Xianning College, Xianning 437005, People's Republic of China.
authors.forename: Shao-Fa
authors.initials: SF
authors.lastname: Sun
authors.forename: Ling-Li
authors.initials: LL
authors.lastname: Pan
authors.forename: Cheng-Ming
authors.initials: CM
authors.lastname: Wang
authors.forename: Han-Na
authors.initials: HN
authors.lastname: Hou
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
mesh_terms: D006801:Humans; D016566:Organoselenium Compounds / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013816:Thermodynamics
chemical_list: C518383:4,4'-diselenadibenzoic acid; D016566:Organoselenium Compounds; D012709:Serum Albumin