Gain of chromosome X in prostatic atrophy detected by CGH and FISH analyses.
BACKGROUND
Focal atrophy is presumed to be an indirect forerunner of prostate cancer. The aim of this study was to examine genetic alterations in prostate epithelia deriving from atrophic areas and compare these findings with those of cells deriving from paired prostate cancer in the same patient.


METHODS
Formalin fixed paraffin wax-embedded prostatectomy specimens from 20 prostate cancer patients were utilized in this study. Comparative Genomic Hybridization (CGH) was performed on atrophic areas. To validate the CGH results, Fluorescence in Situ Hybridization (FISH) analysis was performed on atrophic areas and paired cancer tissue.


RESULTS
Gain of the whole chromosome X was found as sole aberration in seven (70%) atrophic tissues by CGH. A gain of centromere X was observed in 13 (68.4%) atrophic areas and in 18 (90%) cancer tissues using FISH.


CONCLUSIONS
Our investigation reconfirms the genetical instability of cells of the atrophic acini and attention of relevance of gain of chromosome X in atrophic areas.
Yildiz-Sezer
Seval
S
Institute of Pathology, Innsbruck Medical University, Innsbruck, Austria.
Verdorfer
Irmgard
I
Schäfer
Georg
G
Rogatsch
Hermann
H
Bartsch
Georg
G
Mikuz
Gregor
G
D000328:Adult; D000368:Aged; D001284:Atrophy; D041321:Chromosomes, Human, X; D023281:Genomics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology*; D012729:Sex Chromosome Aberrations