title: [Influence of bifidobacterium DNA on PKC and NF-kappaB in murine macrophages].
abstract: OBJECTIVE
  To explore the influence of DNA of bifidobacteriua adolescence on PKC and NK-kappaB of murine macrophages.
  
  
  METHODS
  The fluorescent intensity of PKCalpha, PKCbetaI, PKCbetaII, PKCgamma, PKCepsilon and PKCzeta in murine peritoneal macrophages was detected by using laser confocal microscope. The density of NK-kappaB(+) macrophages was detected by immunocytochemical staining.
  
  
  RESULTS
  The average fluorescent intensity of PKCalpha and PKCbetaII produced by mouse peritoneal macrophages in bifidobacterium DNA injection group was markedly higher than that in control group(P<0.01). There was no statistically significant difference of average fluorescent intensity of PKCbetaI, PKCgamma, PKCepsilon and PKCzeta between the two groups (P>0.05). The density of NK-kappaB(+) macrophages in bifidobacterium DNA injection group was markedly higher than that in control group (P<0.01).
  
  
  CONCLUSIONS
  DNA of bifidobacteria adolescence could activate macrophages by promoting the activity of PKCalpha, PKCbetaII and NF-kappaB.
authors.affiliation: Department of Gastroenterology, the Second Clinical Medical Hospital, Jinan University, Shenzhen 518020, China. wangls168@163.com
authors.forename: Li-sheng
authors.initials: LS
authors.lastname: Wang
authors.forename: Ying-xue
authors.initials: YX
authors.lastname: Li
authors.forename: Hui-ming
authors.initials: HM
authors.lastname: Zhu
authors.forename: Zhong-sheng
authors.initials: ZS
authors.lastname: Zhu
authors.forename: Xiao-dong
authors.initials: XD
authors.lastname: Ma
mesh_terms: D000818:Animals; D001644:Bifidobacterium / Q000235:genetics*; D004269:DNA, Bacterial / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018613:Microscopy, Confocal; D016328:NF-kappa B / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D051571:Protein Kinase C-alpha / Q000378:metabolism; D051744:Protein Kinase C-epsilon / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D016328:NF-kappa B; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha; D051744:Protein Kinase C-epsilon