Nanopore sensor for fast label-free detection of short double-stranded DNAs.
Functionalizing surface enhanced the molecular sensing ability of a fabricated nanopore by increasing the translocation duration time for a short double-stranded DNA. The surface of nanopore was derivatized with gamma-aminopropyltriethoxysilane and the positively charged surface attracted DNA molecules when they were in the vicinity of nanopore. The translocation duration time of DNA increased due to the strong electrostatic interaction and it enabled us to detect a short double-stranded DNA (<1 kbp) that is under the size limit of a conventional solid state nanopore sensor. Both 539 and 910 bp double-stranded DNAs were analyzed with the surface functionalized nanopore and their translocation kinetics are presented in this work. The new feature of the surface modified nanopore that can detect short double-stranded DNA molecules could readily be applied for a rapid label-free diagnostic analysis in a Lab-On-a-Chip type DNA sensor.
Kim
Young-Rok
YR
Bio Device Research Laboratory, Samsung Advanced Institute of Technology, Mt. 14-1, Nongseo-Dong, Giheung-Gu, Yongin-Si 449-712, South Korea.
Min
Junhong
J
Lee
In-Ho
IH
Kim
Suhyeun
S
Kim
Ah-Gi
AG
Kim
Kuihyun
K
Namkoong
Kak
K
Ko
Christopher
C
D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis*; D049329:Nanostructures
D004247:DNA