Clinically localised prostate cancer is microsatellite stable.
OBJECTIVE
To determine the frequency of microsatellite instability (MSI) change with mono-, di- and tetranucleotide markers in clinically localized prostate cancer, and to correlate those markers with clinical and pathological variables.


METHODS
Two forms of MSI have been described in human cancer: MSI typical of hereditary nonpolyposis colon cancer, defined with mono- and dinucleotide repeat MS; and a second variety of MSI is best seen at selective tetranucleotide repeats, i.e. elevated microsatellite alterations at select tetranucleotides (EMAST). Prostate specimens were taken from 50 patients. The MS analysis used the Bethesda consensus panel (BCP) and four tetranucleotide loci shown to detect the presence of EMAST.


RESULTS
All but four tumours were stable for the 14 loci investigated. There were two (4%) cases with adenomatous polyposis coli (APC) instability among the BCP markers and the same instability rate (4%) amongst the EMAST markers. These four tumours were all unstable at one locus of the 10 markers of the BCP that classified them as MS stable.


CONCLUSIONS
The MSI related to a mismatch repair deficiency or to the EMAST does not seem to be important in prostate cancer in the early stages of the disease.
Azzouzi
Abdel-Rahmene
AR
Service d'Urologie, CHU d'Angers, Centre de Recherche pour les Pathologies Protatiques, Université Paris VII, Paris, France, and Department of Pathology, Royal Hallamshire Hospital, Sheffield, UK. arazzouzi@chu-angers.fr
Catto
James W F
JW
Rehman
Ishtiaq
I
Larre
Stephane
S
Roupret
Morgan
M
Feeley
Kenneth M
KM
Cussenot
Oliver
O
Meuth
Mark
M
Hamdy
Freddie C
FC
D053843:DNA Mismatch Repair; D006801:Humans; D008297:Male; D053842:Microsatellite Instability; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology