title: Heterologous production of albicidin: a promising approach to overproducing and characterizing this potent inhibitor of DNA gyrase.
abstract: The phytotoxin and polyketide antibiotic albicidin produced by Xanthomonas albilineans is a highly potent DNA gyrase inhibitor. Low yields of albicidin production have slowed studies of its chemical structure. Heterologous expression of albicidin biosynthetic genes in X. axonopodis pv. vesicatoria resulted in a sixfold increase in albicidin production, offering promising strategies for engineering overproduction.
authors.affiliation: Centre de Coopération Internationale en Recherche Agronomique pour le Développement, UMR Agro.M-Cirad-Inra BGPI, Campus International de Baillarguet, TA 41/K, 34398 Montpellier Cedex 5, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Vivien
authors.forename: Delphine
authors.initials: D
authors.lastname: Pitorre
authors.forename: Stéphane
authors.initials: S
authors.lastname: Cociancich
authors.forename: Isabelle
authors.initials: I
authors.lastname: Pieretti
authors.forename: Dean W
authors.initials: DW
authors.lastname: Gabriel
authors.forename: Philippe C
authors.initials: PC
authors.lastname: Rott
authors.forename: Monique
authors.initials: M
authors.lastname: Royer
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics*; D009930:Organic Chemicals / Q000378:metabolism / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics* / Q000378:metabolism; D059005:Topoisomerase II Inhibitors; D014974:Xanthomonas / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D009930:Organic Chemicals; D059005:Topoisomerase II Inhibitors; C046156:albicidin