MPAI (mass probes aided ionization) method for total analysis of biomolecules by mass spectrometry.
We have designed and synthesized various mass probes, which enable us to effectively ionize various molecules to be detected with mass spectrometry. We call the ionization method using mass probes the "MPAI (mass probes aided ionization)" method. We aim at the sensitive detection of various biological molecules, and also the detection of bio-molecules by a single mass spectrometry serially without changing the mechanical settings. Here, we review mass probes for small molecules with various functional groups and mass probes for proteins. Further, we introduce newly developed mass probes for proteins for highly sensitive detection.
Honda
Aki
A
Department of Applied Chemistry, Keio University, Yokohama, Kanagawa, Japan.
Hayashi
Shinichiro
S
Hifumi
Hiroki
H
Honma
Yuya
Y
Tanji
Noriyuki
N
Iwasawa
Naoko
N
Suzuki
Yoshio
Y
Suzuki
Koji
K
D000818:Animals; D006801:Humans; D007202:Indicators and Reagents; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D009696:Nucleic Acids / Q000032:analysis*; D009711:Nucleotides / Q000032:analysis; D011506:Proteins / Q000032:analysis*
D007202:Indicators and Reagents; D009696:Nucleic Acids; D009711:Nucleotides; D011506:Proteins