Multifunctional polyelectrolyte multilayer films: combining mechanical resistance, biodegradability, and bioactivity.
Cross-linked polyelectrolyte multilayer films (CL PEM) have an increased rigidity and are mechanically more resistant than native (e.g., uncrosslinked) films. However, they are still biodegradable, which make them interesting candidates for biomedical applications. In this study, CL PEM films have been explored for their multifunctional properties as (i) mechanically resistant, (ii) biodegradable, and (iii) bioactive films. Toward this end, we investigated drug loading into CL chitosan/hyaluronan (CHI/HA) and poly(L-lysine)/hyaluronan (PLL/HA) films by simple diffusion of the drugs. Sodium diclofenac and paclitaxel were chosen as model drugs and were successfully loaded into the films. The effect of varying the number of layers in the (CHI/HA) films as well as the cross-linker concentration on diclofenac loading were studied. Diclofenac was released from the film in about 10 h. Paclitaxel was also found to diffuse within CL films. Its activity was maintained after loading in the CL films, and cellular viability could be reduced by about 55% over 3 days. Such a simple approach may be applied to other types of cross-linked films and to other drugs. These results prove that it is possible to design multifunctional multilayer films that combine mechanical resistance, biodegradability, and bioactivity properties into a single PEM architecture.
Schneider
Aurore
A
Institut National de la Santé et de la Recherche Médicale, Unité 595, Faculté de Chirurgie Dentaire, Université Louis Pasteur, 11 rue Humann, 67085 Strasbourg Cedex, France.
Vodouhê
Constant
C
Richert
Ludovic
L
Francius
Gregory
G
Le Guen
Erell
E
Schaaf
Pierre
P
Voegel
Jean-Claude
JC
Frisch
Benoît
B
Picart
Catherine
C
D000327:Adsorption; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D001673:Biodegradation, Environmental; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004008:Diclofenac / Q000494:pharmacology; D004573:Electrolytes / Q000737:chemistry*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry; D046911:Macromolecular Substances / Q000737:chemistry; D018625:Microscopy, Atomic Force; D011107:Polylysine / Q000737:chemistry; D013314:Stress, Mechanical; D013718:Tensile Strength
D000894:Anti-Inflammatory Agents, Non-Steroidal; D003432:Cross-Linking Reagents; D004573:Electrolytes; D046911:Macromolecular Substances; D004008:Diclofenac; D011107:Polylysine; D006820:Hyaluronic Acid; D048271:Chitosan