[Porcine MSC-derived CLC induced by rabbit's cardiomyocytes extract did not induce immune reaction in vivo].
OBJECTIVE
To compare the in vivo immune reaction of transplanting porcine MSC-derived CLC with rabbit cardiomyocytes extracts induced differentiation or in vitro cultured porcine MSC.


METHODS
After injecting the MSC-derived CLC or MSC to the original porcines, the number of CD4+, CD8+ T cells were determined by flow cytometry. The serum concentrations of IL-2, IL-4 were measured by ELISA, and the porcine spleen lymphocyte CTL cytotoxicity was determined by Cell Counting Kit-8 Assay.


RESULTS
The numbers of CD4+ and CD8+ T cells, the serum concentrations of IL-2, IL-4 and spleen lymphocyte CTL cytotoxicity were all similar in porcines received MSC-derived CLC induced by rabbit's CMs extract or MSC transplantation.


CONCLUSIONS
The porcine MSC-derived CLC induced by rabbit's CMs extract did not induce extra immune reaction when injected back to the original porcine.
Cao
Xin-Xin
XX
Department of Cardiology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.
Dai
Yu-Hua
YH
Zhang
Shu-Yang
SY
Chen
Lian-Feng
LF
Lai
Jin-Zhi
JZ
Yan
Xiao-Wei
XW
D000818:Animals; D000911:Antibodies, Monoclonal; D001854:Bone Marrow Cells / Q000276:immunology*; D016026:Bone Marrow Transplantation / Q000276:immunology*; D015704:CD4 Antigens / Q000276:immunology; D016516:CD4-CD8 Ratio; D016827:CD8 Antigens / Q000276:immunology; D005260:Female; D008297:Male; D032383:Myocytes, Cardiac / Q000166:cytology*; D011817:Rabbits; D013552:Swine; D013556:Swine, Miniature
D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D016827:CD8 Antigens