Stimulation of saponin production in Panax ginseng hairy roots by two oligosaccharides from Paris polyphylla var. yunnanensis.
Two oligosaccharides, a heptasaccharide (HS) and an octasaccharide (OS), isolated from Paris polyphylla var. yunnanensis, stimulated the growth and saponin accumulation of Panax ginseng hairy roots at 5-30 mg l(-1). HS and OS at 30 mg l(-1), fed separately to hairy root cultures at 10 days post-inoculation, increased the root biomass dry weight by more than 70% to approximately 20 g l(-1) from 13 g l(-1) and the total saponin content of roots by more than 1-fold to approximately 3.5% from 1.6% (w/w). The results suggest that the two oligosaccharides may have plant growth-regulatory activity in plant tissue cultures.
Zhou
Ligang
L
College of Agronomy and Biotechnology, China Agricultural University, Beijing, China.
Cao
Xiaodong
X
Zhang
Ruifen
R
Peng
Youliang
Y
Zhao
Shoujing
S
Wu
Jianyong
J
D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D019684:Magnoliopsida / Q000378:metabolism*; D009844:Oligosaccharides / Q000008:administration & dosage*; D005894:Panax / Q000187:drug effects / Q000502:physiology*; D018517:Plant Roots / Q000187:drug effects / Q000502:physiology*; D012503:Saponins / Q000378:metabolism*
D009844:Oligosaccharides; D012503:Saponins