title: Simple proteomic checks for detecting noncoding RNA.
abstract: Proper validation can accelerate sequence-based discovery of proteins and protein-coding genes. Databases currently contain a backlog of experimentally unverified gene models and tentative assignments of observed transcripts to coding or noncoding RNA. We present and apply a general principle, founded on base composition and the genetic code and validated here by bulk 2-D gels, that can improve the reliability of such classifications and of the algorithms or pipelines that lead to them.
authors.affiliation: Atelier de Génomique Comparative, Genoscope, Centre National de Séquençage, Evry, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Cruveiller
authors.forename: Oliver
authors.initials: O
authors.lastname: Clay
authors.forename: Kamel
authors.initials: K
authors.lastname: Jabbari
authors.forename: Giorgio
authors.initials: G
authors.lastname: Bernardi
mesh_terms: D001482:Base Composition; D015180:Electrophoresis, Gel, Two-Dimensional; D007526:Isoelectric Point; D040901:Proteomics / Q000379:methods; D022661:RNA, Untranslated / Q000032:analysis*
chemical_list: D022661:RNA, Untranslated