title: [Suppression of proliferation of Hep-2 cells by stable expression of anti-sense perlecan cDNA].
abstract: OBJECTIVE
  To study the suppression of proliferation of Hep-2 cells by stable expression of anti-sense perlecan cDNA.
  
  
  METHODS
  In this study, the plasmids of recombination eukaryotic expression vector perlecan anti-sense cDNA (pAP) were transfected into Hep-2 cells by using cationic liposome (lipofectamine 2000) and divided into three groups: non-transfected group, WT group; transfection with no load carrier, neo group; and transfection with the pAP plasmid, pAP group. Semi quantify RT-PCR, western blot assay and MTT assay were used to detected the expression of perlecan mRNA and protein in the three groups; the level of cell proliferation; and the responsivity of basic fibroblast growth factor (bFGF).
  
  
  RESULTS
  It was showed that the expression of perlecan mRNA and protein were significantly reduced in the pAP group compared with WT group and ph beta Apr-neol transfected group ( P < 0.01). In the presence of 1 microg/L of bFGF in low serum (0.1% FCS), the pAP transfected cells showed a reduced proliferation rate (MTT assay) while the wild type cells and ph beta Apr-neol transfected cells grew rapidly.
  
  
  CONCLUSIONS
  The growth of Hep-2 cells could be inhibited significantly by perlecan anti-sense cDNA plasmids transfection.
authors.affiliation: Department of Otolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
authors.forename: Guangli
authors.initials: G
authors.lastname: Chen
authors.forename: Shusheng
authors.initials: S
authors.lastname: Gong
authors.forename: Juan
authors.initials: J
authors.lastname: Ding
authors.forename: Yingpeng
authors.initials: Y
authors.lastname: Liu
authors.forename: Linghui
authors.initials: L
authors.lastname: Luo
authors.forename: Jianting
authors.initials: J
authors.lastname: Wang
authors.forename: Pei
authors.initials: P
authors.lastname: Chen
authors.forename: Kaisheng
authors.initials: K
authors.lastname: Yan
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018076:DNA, Complementary; D005822:Genetic Vectors; D019812:Heparan Sulfate Proteoglycans / Q000235:genetics*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000473:pathology; D010957:Plasmids; D014162:Transfection
chemical_list: D018076:DNA, Complementary; D019812:Heparan Sulfate Proteoglycans; C071927:perlecan