Immunological changes and cytokine gene expression during primary infection with human T-cell leukaemia virus type 1 in squirrel monkeys (Saimiri sciureus).
We have developed an animal model of experimental HTLV-1 infection in Saimiri sciureus monkeys in order to study both the immunological and the virological aspects of the infection. As cytokines expressed by immune cells play an essential role during viral infection, we have studied the correlation between the expression of some Th1/Th2 cytokines, including IL-2, IL-5, IL-6, IL-10, and IFN-gamma, and immunological dynamics during primary and chronic HTLV-1 infection in this model. We first demonstrated that, during primary infection, IFN-gamma, IL-2 and IL-10 are expressed at different times and levels. The expression of these cytokines is concomitant with the increase in the numbers of CD4(+), CD8(+) and CD16(+) cells and with the presence of tax/rex viral mRNA. These data indicate the involvement of various cell types in the antiviral immune response. Subsequently, we showed that peripheral blood mononuclear cells freshly isolated from chronically infected monkeys express IFN-gamma and IL-6 at higher levels than those from uninfected animals. IFN-gamma expression is quantitatively correlated to the proviral load and to the presence of circulating effector T-cells against Tax peptide, as detected by Elispot. Further studies will be needed to determine the effective role of these cytokines and other immune system modulators in the control of viral replication during primary HTLV-1 infection or latency.
Heraud
Jean-Michel
JM
Laboratoire de Rétrovirologie, Institut Pasteur de la Guyane, BP6010, 97306 Cayenne, French Guiana, France.
Merien
Fabrice
F
Mortreux
Frank
F
Mahieux
Renaud
R
Kazanji
Mirdad
M
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis*; D016370:Gene Products, rex / Q000235:genetics; D016356:Gene Products, tax / Q000235:genetics / Q000276:immunology; D015490:HTLV-I Infections / Q000276:immunology*; D015368:Human T-lymphotropic virus 1 / Q000302:isolation & purification; D007963:Leukocytes, Mononuclear; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D011533:Proviruses / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D017452:Receptors, IgG / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012453:Saimiri; D037182:T-Cell Antigen Receptor Specificity; D019562:Viral Load
D016207:Cytokines; D016370:Gene Products, rex; D016356:Gene Products, tax; D012333:RNA, Messenger; D012367:RNA, Viral; D017452:Receptors, IgG