title: Lipolysis is altered in MHC class I zinc-alpha(2)-glycoprotein deficient mice.
abstract: Non-conventional major histocompatibility complex class I molecules are involved in a variety of physiological functions, most at the periphery of the immune system per se. Zinc-alpha(2)-glycoprotein (ZAG), the sole soluble member of this superfamily has been implicated in cachexia, a poorly understood yet life-threatening, severe wasting syndrome. To further ascertain the role of ZAG in lipid metabolism and perhaps the immune system, we inactivated both ZAG alleles by gene targeting in mice. Subjecting these ZAG deficient animals to standard or lipid rich food regimens led to increased body weight in comparison to identically treated wild-type mice. This phenotype appeared to correlate with a significant decrease in adipocytic lipolysis that could not be rescued by several pharmacological agents including beta(3)-adrenoreceptor agonists. Furthermore, in contrast to previously reported data, ZAG was found to be ubiquitously and constitutively expressed, with an especially high level in the mouse liver. No overt immunological phenotype was identified in these animals.
authors.affiliation: Immunogénétique Moléculaire Humaine, Centre de Recherche d'Immunologie et d'Hématologie, Faculté de Médecine, Hôpitaux Universitaires de Strasbourg, 4 Rue Kirschleger, 67085 Strasbourg Cedex, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Rolli
authors.forename: Mirjana
authors.initials: M
authors.lastname: Radosavljevic
authors.forename: Valérie
authors.initials: V
authors.lastname: Astier
authors.forename: Cécile
authors.initials: C
authors.lastname: Macquin
authors.forename: Isabelle
authors.initials: I
authors.lastname: Castan-Laurell
authors.forename: Virgile
authors.initials: V
authors.lastname: Visentin
authors.forename: Charlotte
authors.initials: C
authors.lastname: Guigné
authors.forename: Christian
authors.initials: C
authors.lastname: Carpéné
authors.forename: Philippe
authors.initials: P
authors.lastname: Valet
authors.forename: Susan
authors.initials: S
authors.lastname: Gilfillan
authors.forename: Seiamak
authors.initials: S
authors.lastname: Bahram
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D058667:Adrenergic beta-3 Receptor Agonists; D000483:Alleles; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics; D004041:Dietary Fats / Q000008:administration & dosage; D015870:Gene Expression; D018390:Gene Targeting; D006367:HeLa Cells; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008066:Lipolysis / Q000502:physiology*; D008099:Liver / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D022702:Receptors, Adrenergic, beta-3 / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D029589:Seminal Plasma Proteins / Q000235:genetics / Q000378:metabolism*; D014018:Tissue Distribution; D014162:Transfection; D015430:Weight Gain; D000090203:Zn-Alpha-2-Glycoprotein
chemical_list: D058667:Adrenergic beta-3 Receptor Agonists; D004041:Dietary Fats; D015395:Histocompatibility Antigens Class I; D012333:RNA, Messenger; D022702:Receptors, Adrenergic, beta-3; D011994:Recombinant Proteins; D029589:Seminal Plasma Proteins; D000090203:Zn-Alpha-2-Glycoprotein; D004247:DNA