Establishment and biological characterization of canine histiocytic sarcoma cell lines.
Seven novel cell lines from canine histiocytic sarcoma (HS), three of which were disseminated cutaneous HS and four of which were synovial HS, were established. All of the established cell lines had the same morphological (by light and electron microscopic findings), cytochemical (alpha-naphthyl butyrate esterase-positive), and immunohistochemical (vimentin- and lysozyme-positive, and cyto-keratin-negative) characteristics as the original HS tumor cells. All of the established cell lines injected into nude mice subcutaneously produced solid tumors. Because the established cell lines also showed phagocytic and processing activities, the HS tumor cells appear to originate from the mononuclear phagocytic system cells, despite their differences in locations or organs.
Azakami
Daigo
D
Department of Veterinary Clinical Pathology, Nippon Veterinary and Life Science University, Musashino-Shi, Tokyo, Japan.
Bonkobara
Makoto
M
Washizu
Tsukimi
T
Iida
Akiko
A
Kondo
Mari
M
Kato
Rina
R
Niikura
Yumiko
Y
Iwaki
Shuko
S
Tamahara
Satoshi
S
Matsuki
Naoaki
N
Ono
Kenichiro
K
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D004285:Dogs; D012509:Sarcoma / Q000145:classification / Q000473:pathology / Q000662:veterinary*