Course of Borrelia burgdorferi DNA shedding in urine after treatment.
Diagnosis of Lyme borreliosis by urine polymerase chain reaction (PCR) has been recognized as having better diagnostic sensitivity in patients with erythema migrans than serological methods. We made serial tests with 192 urine specimens from 70 patients with erythema migrans and 60 urine specimens from 21 patients with acrodermatitis chronica atrophicans to evaluate the course of positive urine PCR after antibiotic treatment. Before treatment, urine samples from patients with erythema migrans showed a positive PCR in 27/34 samples (79%), and those from patients with acrodermatitis chronica atrophicans in 7/11 (63%). The specificity of bands was proven by hybridization with GEN-ETI-KTM-DEIA kit in 40/41 samples. Borrelia DNA in urine decreased gradually within the observation period of one year in both patients with erythema migrans and acrodermatitis chronica atrophicans, and persisted without clinical symptoms in 4/45 patients with erythema migrans (8%) after 12 months. Urine PCR can serve as a diagnostic method in early Lyme borreliosis and also in seropositive patients with unclear clinical symptoms.
Aberer
Elisabeth
E
Department of Dermatology, Medical University of Graz, Auenbruggerplatz 8, Austria. elisabeth.aberer@meduni-graz.at
Bergmann
Andreas R
AR
Derler
Anna-Maria
AM
Schmidt
Bruno
B
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D025065:Borrelia burgdorferi / Q000235:genetics / Q000302:isolation & purification*; D002648:Child; D004269:DNA, Bacterial / Q000652:urine*; D015787:Erythema Chronicum Migrans / Q000652:urine*; D005260:Female; D006801:Humans; D008193:Lyme Disease / Q000188:drug therapy / Q000652:urine; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial