title: Effect of the electrical currents generated by the intestinal smooth muscle layers on pancreatic enzyme activity: an in vitro study.
abstract: Gut enzymes in the small intestine are exposed to extremely low electrical currents (ELEC) generated by the smooth muscle. In the present study, the in vitro tests were undertaken to evaluate the effect of these electric currents on the activity of the proteolytic pancreatic digestive enzymes. A simulator generating the typical electrical activity of pig gut was used for these studies. The electric current emitted by the simulator was transmitted to the samples, containing enzyme and its substrate, using platinum plate electrodes. All samples were incubated at 37 degrees C for 1 h. The changes in optical density, corresponding to enzyme activity, in samples stimulated for 1 h with ELEC was compared with that not exposed to ELEC. The obtained results show that the electrical current with the characteristics of the myoelectrical migrating complex (MMC) has an influence on pancreatic enzyme activity. Increased endopeptidase and reduced exopeptidase activity was noticed in samples treated with ELEC. This observation can be of important as analyzed factors which can alter enzymatic activity of the gut, can thus also affect feed/food digestibility.
authors.affiliation: Department of Processing Technology, Sea Fisheries Institute, Gdynia, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Dabek
authors.forename: Paweł
authors.initials: P
authors.lastname: Podgurniak
authors.forename: Jose L Valverde
authors.initials: JL
authors.lastname: Piedra
authors.forename: Sylwia
authors.initials: S
authors.lastname: Szymańczyk
authors.forename: Rafał
authors.initials: R
authors.lastname: Filip
authors.forename: Anna
authors.initials: A
authors.lastname: Wojtasz-Pajak
authors.forename: Eliza
authors.initials: E
authors.lastname: Werpachowska
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Podgurniak
authors.forename: Stefan G
authors.initials: SG
authors.lastname: Pierzynowski
mesh_terms: D000818:Animals; D001586:Benzoylarginine Nitroanilide; D002417:Cattle; D004594:Electrophysiology; D066298:In Vitro Techniques; D007422:Intestines / Q000502:physiology*; D007768:Lactalbumin; D008954:Models, Biological; D009130:Muscle, Smooth / Q000502:physiology*; D016065:Myoelectric Complex, Migrating / Q000502:physiology; D009555:Ninhydrin; D010189:Pancreatic Juice / Q000201:enzymology*; D012710:Serum Albumin, Bovine; D013552:Swine
chemical_list: D012710:Serum Albumin, Bovine; D007768:Lactalbumin; D001586:Benzoylarginine Nitroanilide; D009555:Ninhydrin