[Effect of inhibiting aquaporin-1 on proliferation and apoptosis of the Hep-2 cell].
OBJECTIVE
To explore the effect of inhibiting aquaporin-1 (AQP-1) on proliferation and apoptosis of the Hep-2 cell.


METHODS
The expression of AQP-1 in Hep-2 cell was identified through immunohistochemistry. After using acetazolamide, an antagonist of AQP-1, the assay of cell proliferation, apoptosis and the expression of AQP-1 were performed by microculture tetrazolium salt(MTT) and flow cytometry, TUNEL respectively.


RESULTS
The expression of AQP-1 is mainly found on membrane of Hep-2 cell and the expression is significantly reduced when the concentration of acetazolamide is 5 x 10(-5) mol/L. Noteworthily, inhibiting AQP-1 by acetazolamide, the rate of cell growth and cell apoptosis significantly increases with the time prolong. It showed time-dependent phenomena. There is positive relationship obviously between cell apoptosis rate and inhibition rate of the AQP-1 protein expression.


CONCLUSIONS
The inhibiting AQP-1 with acetazolamide may significantly induce apoptosis of Hep-2 cell. It suggest that AQP-1 may be as new target of therapy of laryngeal cancer.
Guan
Guimei
G
Department of Otolaryngology-Head and Neck Surgery Chinese-Japanese Union Hospital, Bethune Faculty of Medicine, Jilin University, Changchun 130031, China.
Dong
Zhen
Z
D000086:Acetazolamide / Q000494:pharmacology; D017209:Apoptosis; D051398:Aquaporin 1 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology
C496020:AQP1 protein, human; D051398:Aquaporin 1; D000086:Acetazolamide