Using hooves for high-resolution isotopic reconstruction of bovine dietary history.
The objective of this study was to ascertain whether sequential sampling and isotopic analysis of bovine hooves could be used to reconstruct the dietary history of cattle. A controlled, on-farm experiment was conducted in which cattle were switched from a barley-based diet to an isotopically different diet incorporating maize, urea and seaweed (the isotopic spacing between diets was 13.6 per thousand for delta(13)C and 8.0 per thousand for delta(15)N) and maintained on that diet for 168 days. Postmortem sampling of the cleaned anterior wall of the lateral, left front claw was carried out on five individuals using a micro-drilling technique. From the first 60 mm of each claw, up to 41 samples with a spacing between them of less than 1 mm were collected. Bands were less than 1 mm deep and had a mean width of 1.2 mm. The hoof keratin showed a rapid increase followed by a slower increase in its delta(13)C and delta(15)N values following the diet switch, suggesting that C and N in hoof keratin originate from more than one pool. However, the response of the N isotope composition of the hoof was somewhat delayed compared with that of C. Estimated mean hoof growth rates for these cattle were 10.5 +/- 2.3 mm per month and 6.7 +/- 1.0 mm per month (+/-SD, n = 5) when receiving the barley-based transition diet and the maize-based experimental diet, respectively. These values are considerably higher than previous estimates obtained by visual methods and they suggest that diet may have a greater influence on hoof growth rates than seasonality. These results demonstrate that hooves are a suitable incremental tissue for high-resolution isotopic reconstruction of the dietary history of bovine animals.
Harrison
Sabine M
SM
UCD School of Agriculture, Food Science and Veterinary Medicine, Agriculture and Food Science Centre, University College Dublin, Belfield, Dublin 4, Ireland. sabine.harrison@ucd.ie
Zazzo
Antoine
A
Bahar
Bojlul
B
Monahan
Frank J
FJ
Moloney
Aidan P
AP
Scrimgeour
Charlie M
CM
Schmidt
Olaf
O
D000821:Animal Feed / Q000032:analysis*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001835:Body Weight; D002247:Carbon Isotopes / Q000032:analysis; D002417:Cattle; D004032:Diet; D006724:Hoof and Claw / Q000737:chemistry* / Q000254:growth & development; D001467:Hordeum / Q000378:metabolism; D007554:Isotopes; D007633:Keratins / Q000737:chemistry*; D008297:Male; D009587:Nitrogen Isotopes / Q000032:analysis; D012624:Seaweed / Q000378:metabolism; D014508:Urea / Q000378:metabolism; D003313:Zea mays / Q000378:metabolism
D002247:Carbon Isotopes; D007554:Isotopes; D009587:Nitrogen Isotopes; D007633:Keratins; D014508:Urea