[Establishment and biological characteristics of lung cancer cell line XWLC-05].
OBJECTIVE
The incidence of lung cancer is high at Xuanwei, Yunnan Province, at where the mortality rate of this disease in women is the highest in China. This study was to establish a Xuanwei woman lung adenocarcinoma cell line, and provide an in vitro experimental model for the study of preventing and treating lung cancer.


METHODS
The cells derived from a surgical specimen of a woman patient with lung cancer were primarily cultured. The biological characteristics of the cell line were studied with light and electron microscopes, determination of doubling time and growth curve, culturing in soft agar, flow cytometry (FCM), chromosome and G-band detection, c-12 multiple tumor markers detection, and inoculation in mice.


RESULTS
Morphologic study, proliferation dynamics, and invasive growth showed that the cultured cells have malignant characteristics. Their chromosome numbers ranged from 55 to 69, with a mode number of 60-63. The tumor formation rate in mice was 100% after axillary transplantation of the cells; the morphology of the tumor cells was similar to that of the pathologic specimen of the patient. The cell line was named XWLC-05.


CONCLUSIONS
According to the newest rules of establishing a cell line in vitro, XWLC-05 is proved to be a new cell line of human lung adenocarcinoma.
Yan
Feng-Cai
FC
Department of Pathology, Kunming Medical College, Kunming, Yunnan, 650031, P. R. China.
Wang
Qin-Qin
QQ
Ruan
Yong-Hua
YH
Ma
Li-Ju
LJ
Jia
Jian-Tao
JT
Jin
Ke-Wei
KW
D000230:Adenocarcinoma / Q000737:chemistry / Q000235:genetics / Q000473:pathology*; D000368:Aged; D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D018394:CA-125 Antigen / Q000032:analysis; D045744:Cell Line, Tumor / Q000648:ultrastructure; D002681:China; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000737:chemistry / Q000235:genetics / Q000473:pathology*; D051379:Mice; D009368:Neoplasm Transplantation; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D011123:Polyploidy
D014408:Biomarkers, Tumor; D018394:CA-125 Antigen; D010751:Phosphopyruvate Hydratase