title: Anion- and cation-exchange MicroHPLC utilizing poly(methacrylates)-coated monolithic silica capillary columns.
abstract: A novel method was developed for the preparation of highly efficient anion- and cation-exchange microHPLC columns using an on-column polymerization of methacrylates having amine or sulfonic acid functional groups onto monolithic silica capillary columns modified with 3-methacryloxypropyltriethoxysilane as the anchor groups. The chromatographic evaluation of the columns using nucleic acids, nucleotides, and inorganic anions as samples showed the characteristics of the ion-exchange-type stationary phases. These columns exhibited higher separation efficiency when compared with the conventional particle-packed columns. A capillary column for the simultaneous anion- and cation-exchange separation could be prepared by a step-by-step functionalization. The advantages of this column preparation will include: (1) no need of column packing; (2) no need of the preparation of silane reagents possessing anion- and cation-exchange functionalities; (3) the amount of immobilized polymer could be controlled by changing polymerization conditions. These columns should be suitable for the separation of biologically active compounds by the microHPLC modes.
authors.affiliation: Department of Biomolecular Engineering, Kyoto Institute of Technology, Japan. ikegami@kit.ac.jp
authors.forename: Tohru
authors.initials: T
authors.lastname: Ikegami
authors.forename: Jun
authors.initials: J
authors.lastname: Ichimaru
authors.forename: Wataru
authors.initials: W
authors.lastname: Kajiwara
authors.forename: Nozomi
authors.initials: N
authors.lastname: Nagasawa
authors.forename: Ken
authors.initials: K
authors.lastname: Hosoya
authors.forename: Nobuo
authors.initials: N
authors.lastname: Tanaka
mesh_terms: D000837:Anion Exchange Resins; D002411:Cation Exchange Resins; D002848:Chromatography, DEAE-Cellulose; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D011109:Polymethacrylic Acids / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D000837:Anion Exchange Resins; D002411:Cation Exchange Resins; D007202:Indicators and Reagents; D011109:Polymethacrylic Acids; C030613:polymethacrylic acid; D012822:Silicon Dioxide