title: Synergistic effects of pravastatin and pioglitazone in renal tubular epithelial cells induced by transforming growth factor-beta1.
abstract: Recent studies suggest that treatment with PPAR-gamma agonists and statins have beneficial effects on renal disease. However, the combined effects of PPAR-gamma agonists and statins in human renal epithelial cells are unknown. Our present study revealed that there were synergistic effects of pravastatin and pioglitazone in the expression of alpha-smooth muscle actin (alpha-SMA), connective tissue growth factor (CTGF), fibronectin (FN), plasminogen activator inhibitor-1 (PAI-1) and collagen 1 in human renal proximal tubular epithelial cells induced by transforming growth factor-beta 1 (TGF-beta1). The beneficial effects of combined therapy against renal tubular epithelial cell injury are attributed, at least in part, to the inhibition of transdifferentiation, extracellular matrix deposition and cytokine production.
authors.affiliation: Department of Cardio, Renal and Cerebrovascular Medicine, Faculty of Medicine, Kagawa University, Kagawa, Japan. jlwei525@yahoo.com.cn
authors.forename: Jia-li
authors.initials: JL
authors.lastname: Wei
authors.forename: Chun-yang
authors.initials: CY
authors.lastname: Ma
authors.forename: Yang-De
authors.initials: YD
authors.lastname: Zhang
authors.forename: Yiming
authors.initials: Y
authors.lastname: Li
mesh_terms: D000199:Actins / Q000235:genetics; D002460:Cell Line; D024042:Collagen Type I / Q000235:genetics; D055513:Connective Tissue Growth Factor; D005353:Fibronectins / Q000235:genetics; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics; D007687:Kidney Tubules, Proximal / Q000166:cytology* / Q000187:drug effects; D032389:Myocytes, Smooth Muscle / Q000166:cytology; D047495:PPAR gamma / Q000819:agonists; D000077205:Pioglitazone; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics; D017035:Pravastatin / Q000494:pharmacology*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D045162:Thiazolidinediones / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*; D019459:Urothelium / Q000166:cytology* / Q000187:drug effects
chemical_list: D000199:Actins; C526317:CCN2 protein, human; D024042:Collagen Type I; D005353:Fibronectins; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D047495:PPAR gamma; D017395:Plasminogen Activator Inhibitor 1; D045162:Thiazolidinediones; D053773:Transforming Growth Factor beta1; D055513:Connective Tissue Growth Factor; D012313:RNA; D017035:Pravastatin; D000077205:Pioglitazone