Methamphetamine downregulates peroxiredoxins in rat pheochromocytoma cells.
Methamphetamine (METH) is an abusive psychostimulant that induces neuronal cell death/degeneration in experimental animals and humans. METH-induced apoptosis in rat pheochromocytoma cells was utilized to study the neurotoxic mechanism. During METH intoxication, we found that peroxiredoxins and thioredoxins/thioredoxin reductases (peroxiredoxin reducing systems) which are known to prevent oxidative stress and apoptosis were differentially downregulated and upregulated, respectively. We also found not only the free radicals but also the oxidative forms of peroxiredoxin and thioredoxin were increased, indicating the dysfunction of these enzymes. Thus, METH-induced differential regulation and oxidation of peroxiredoxins and thioredoxin may be an important mechanism for apoptosis.
Chen
Han-Min
HM
Department of Life Science, Fu-Jen Catholic University, Taiwan, ROC.
Lee
Yi-Chao
YC
Huang
Chuen-Lin
CL
Liu
Hui-Kang
HK
Liao
Wei-Chen
WC
Lai
Wen-Lin
WL
Lin
Yen-Ru
YR
Huang
Nai-Kuei
NK
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D008694:Methamphetamine / Q000494:pharmacology*; D016716:PC12 Cells; D010544:Peroxidases / Q000378:metabolism*; D054464:Peroxiredoxins; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects*; D013879:Thioredoxins / Q000378:metabolism*
D008694:Methamphetamine; D013879:Thioredoxins; D010544:Peroxidases; D054464:Peroxiredoxins