title: Mechanisms of abnormal calcium homeostasis in mutations responsible for catecholaminergic polymorphic ventricular tachycardia.
abstract: Catecholaminergic polymorphic ventricular tachycardia is a heritable arrhythmia unmasked by exertion or stress and is characterized by triggered activity and sudden cardiac death. In this study, we simulated mutations in 2 genes linked to catecholaminergic polymorphic ventricular tachycardia, the first located in calsequestrin (CSQN2) and the second in the ryanodine receptor (RyR2). The aim of the study was to investigate the mechanistic basis for spontaneous Ca2+ release events that lead to delayed afterdepolarizations in affected patients. Sarcoplasmic reticulum (SR) luminal Ca2+ sensing was incorporated into a model of the human ventricular myocyte, and CSQN2 mutations were modeled by simulating disrupted RyR2 luminal Ca2+ sensing. In voltage-clamp mode, the mutant CSQN2 model recapitulated the smaller calcium transients, smaller time to peak calcium transient, and accelerated recovery from inactivation seen in experiments. In current clamp mode, in the presence of beta stimulation, we observed delayed afterdepolarizations, suggesting that accelerated recovery of RyR2 induced by impaired luminal Ca2+ sensing underlies the triggered activity observed in mutant CSQN2-expressing myocytes. In current-clamp mode, in a model of mutant RyR2 that is characterized by reduced FKBP12.6 binding to the RyR2 on beta stimulation, the impaired coupled gating characteristic of these mutations was modeled by reducing cooperativity of RyR2 activation. In current-clamp mode, the mutant RyR2 model exhibited increased diastolic RyR2 open probability that resulted in formation of delayed afterdepolarizations. In conclusion, these minimal order models of mutant CSQN2 and RyR2 provide plausible mechanisms by which defects in RyR2 gating may lead to the cellular triggers for arrhythmia, with implications for the development of targeted therapy.
authors.affiliation: Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, MA 02129, USA.
authors.forename: Vivek
authors.initials: V
authors.lastname: Iyer
authors.forename: Roger J
authors.initials: RJ
authors.lastname: Hajjar
authors.forename: Antonis A
authors.initials: AA
authors.lastname: Armoundas
mesh_terms: D002118:Calcium / Q000502:physiology; D002155:Calsequestrin / Q000235:genetics / Q000378:metabolism; D006706:Homeostasis / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008954:Models, Biological; D009154:Mutation; D011110:Polymorphism, Genetic / Q000235:genetics*; D017983:Receptors, Catecholamine / Q000235:genetics* / Q000502:physiology; D019837:Ryanodine Receptor Calcium Release Channel / Q000235:genetics / Q000378:metabolism; D017180:Tachycardia, Ventricular / Q000235:genetics* / Q000378:metabolism
chemical_list: C443109:CASQ2 protein, human; D002155:Calsequestrin; D017983:Receptors, Catecholamine; D019837:Ryanodine Receptor Calcium Release Channel; D002118:Calcium