Stable expression of foot-and-mouth disease virus protein VP1 fused with cholera toxin B subunit in the potato (Solanum tuberosum).
The expression vector, pBI121CTBVP1, containing the fusion of the foot and mouth disease virus (FMDV) VP1 gene and the cholera toxin B subunit (CTB) gene was constructed by fused PCR and transferred into potato (Solanum tuberosum L.) by Agrobacterium-mediated transformation. Transformed plants were obtained by selecting on kanamycin-resistant medium strictly and regenerated. The transgenic plantlets were identified by PCR, Southern-blot and the production of fused protein was confirmed and quantified by Western-blot and ELISA assays. The results showed that the fused genes were expressed stablely under the control of specific-tuber patatin promoter. The expressed fused proteins have a certain degree of immunogenicity.
He
Dong-Mei
DM
Department of Biotechnology, College of Life Science, Zhejiang University, Hangzhou 310027, China.
Qian
Kai-Xian
KX
Shen
Gui-Fang
GF
Li
Yi-Nü
YN
Zhang
Zhi-Fang
ZF
Su
Zhong-Liang
ZL
Shao
Hong-Bo
HB
D015139:Blotting, Southern; D015153:Blotting, Western; D002265:Carboxylic Ester Hydrolases / Q000235:genetics; D002772:Cholera Toxin / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics*; D015870:Gene Expression; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D011198:Solanum tuberosum / Q000235:genetics* / Q000378:metabolism
D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C043222:patatin protein, Solanum tuberosum; D002772:Cholera Toxin; D002265:Carboxylic Ester Hydrolases