Differential antigen processing by dendritic cell subsets in vivo.
Dendritic cells (DCs) process and present self and foreign antigens to induce tolerance or immunity. In vitro models suggest that induction of immunity is controlled by regulating the presentation of antigen, but little is known about how DCs control antigen presentation in vivo. To examine antigen processing and presentation in vivo, we specifically targeted antigens to two major subsets of DCs by using chimeric monoclonal antibodies. Unlike CD8+ DCs that express the cell surface protein CD205, CD8- DCs, which are positive for the 33D1 antigen, are specialized for presentation on major histocompatibility complex (MHC) class II. This difference in antigen processing is intrinsic to the DC subsets and is associated with increased expression of proteins involved in MHC processing.
Dudziak
Diana
D
Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10021, USA.
Kamphorst
Alice O
AO
Heidkamp
Gordon F
GF
Buchholz
Veit R
VR
Trumpfheller
Christine
C
Yamazaki
Sayuri
S
Cheong
Cheolho
C
Liu
Kang
K
Lee
Han-Woong
HW
Park
Chae Gyu
CG
Steinman
Ralph M
RM
Nussenzweig
Michel C
MC
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D017951:Antigen Presentation; D015703:Antigens, CD / Q000032:analysis / Q000276:immunology; D001483:Base Sequence; D016827:CD8 Antigens / Q000032:analysis / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D037181:Lectins, C-Type / Q000032:analysis / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D015778:Minor Histocompatibility Antigens; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D011956:Receptors, Cell Surface / Q000032:analysis / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D016827:CD8 Antigens; C093569:DEC-205 receptor; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D037181:Lectins, C-Type; D015778:Minor Histocompatibility Antigens; D011956:Receptors, Cell Surface