hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes.
Complete sets of cloned protein-encoding open reading frames (ORFs), or ORFeomes, are essential tools for large-scale proteomics and systems biology studies. Here we describe human ORFeome version 3.1 (hORFeome v3.1), currently the largest publicly available resource of full-length human ORFs (available at ). Generated by Gateway recombinational cloning, this collection contains 12,212 ORFs, representing 10,214 human genes, and corresponds to a 51% expansion of the original hORFeome v1.1. An online human ORFeome database, hORFDB, was built and serves as the central repository for all cloned human ORFs (http://horfdb.dfci.harvard.edu). This expansion of the original ORFeome resource greatly increases the potential experimental search space for large-scale proteomics studies, which will lead to the generation of more comprehensive datasets.
Lamesch
Philippe
P
Center for Cancer Systems Biology and Department of Cancer Biology, Dana-Farber Cancer Institute, and Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
Li
Ning
N
Milstein
Stuart
S
Fan
Changyu
C
Hao
Tong
T
Szabo
Gabor
G
Hu
Zhenjun
Z
Venkatesan
Kavitha
K
Bethel
Graeme
G
Martin
Paul
P
Rogers
Jane
J
Lawlor
Stephanie
S
McLaren
Stuart
S
Dricot
Amélie
A
Borick
Heather
H
Cusick
Michael E
ME
Vandenhaute
Jean
J
Dunham
Ian
I
Hill
David E
DE
Vidal
Marc
M
D000818:Animals; D002877:Chromosomes, Human; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary; D030561:Databases, Nucleic Acid; D020022:Genetic Predisposition to Disease; D015894:Genome, Human; D006801:Humans; D020407:Internet; D016366:Open Reading Frames; D040901:Proteomics; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary