title: NPY regulates human endocardial endothelial cell function.
abstract: Growing evidence suggests that endocardial endothelial cells (EECs) may play an important role in the regulation of cardiac function by releasing several cardioactive factors such as endothelin-1 (ET-1), Angiotensin II (Ang II) and nitric oxide (NO). In our laboratory, we demonstrated that similar to ET-1, EECs do possess different types of NPY receptors, specifically Y(1) and Y(2) receptors. Furthermore, activation of these receptors was found to increase the steady-state level of intracellular free Ca(2+) in EECs and the frequency of beating of cardiomyocytes. In addition, NPY was also found to be present in EECs, and an increase of steady-state intracellular free Ca(2+) induced the release of this peptide from these cells. Thus, similar to ET-1, NPY seems to be released from EECs and this peptide seems to regulate excitation-secretion of these cells as well as excitation-contraction coupling of ventricular cardiomyocytes.
authors.affiliation: Department of Anatomy & Cell Biology, Faculty of Medicine, Université de Sherbrooke, Sherbrooke, 3001-12th Avenue North, Québec, Canada J1H 5N4.
authors.forename: Dima
authors.initials: D
authors.lastname: Abdel-Samad
authors.forename: Danielle
authors.initials: D
authors.lastname: Jacques
authors.forename: Claudine
authors.initials: C
authors.lastname: Perreault
authors.forename: Chantal
authors.initials: C
authors.lastname: Provost
mesh_terms: D000818:Animals; D002642:Chick Embryo; D004699:Endocardium / Q000166:cytology*; D004727:Endothelium / Q000166:cytology*; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D009478:Neuropeptide Y / Q000502:physiology*; D011863:Radioimmunoassay
chemical_list: D009478:Neuropeptide Y