[The evaluation criterions about Caco-2 cell model].
OBJECTIVE
To establish and evaluate the Caco-2 cell model.


METHODS
Caco-2 cell model was evaluated by morphology feature, TEER alkaline phosphatase and the transport of Propranolol.


RESULTS
The determined values were corresponding to the references. The integrality, permeability and the expression of alkaline phosphatase in the cell model were superior.


CONCLUSIONS
The established Caco-2 cell model can be used to study the absorption mechanism of oral administration drug.
Ma
Yan
Y
School of Life Science, Sun Yat-sen University, Guangzhou 510275, China.
Li
Pei-bo
PB
Su
Wei-wei
WW
Chen
Da-wei
DW
D000469:Alkaline Phosphatase / Q000378:metabolism; D001692:Biological Transport / Q000502:physiology; D018938:Caco-2 Cells; D018929:Cell Culture Techniques / Q000379:methods*; D002463:Cell Membrane Permeability; D049109:Cell Proliferation; D003470:Culture Media / Q000494:pharmacology; D017097:Electric Impedance; D005069:Evaluation Studies as Topic; D006801:Humans; D007408:Intestinal Absorption; D008954:Models, Biological; D010539:Permeability; D011433:Propranolol / Q000378:metabolism
D003470:Culture Media; D011433:Propranolol; D000469:Alkaline Phosphatase