Binding of host extracellular matrix proteins to Mycoplasma fermentans and its effect on adherence to, and invasion of HeLa cells.
In the present study, we show that intact Mycoplasma fermentans cells have a wealth of adhesive interactions with components of the extracellular matrix. Mycoplasma fermentans intensively bind plasminogen, and to a lesser extent, fibronectin, heparin, and laminin. The binding of collagen type III, IV, or V was low. The binding of plasminogen, collagen type III, or collagen type V markedly enhanced the adherence of M. fermentans to HeLa cells, whereas the binding of fibronectin, heparin, laminin, or collagen IV induced only a small effect on mycoplasma adherence. Utilizing plasminogen-treated M. fermentans preparations, we detected microorganisms within host HeLa cells by the gentamicin protection assay or by confocal laser scanning microscopy of immunofluorescent preparations. However, no intracellular M. fermentans was detected when M. fermentans preparations treated with fibronectin, heparin, laminin, or collagen type III, IV, or V were utilized.
Yavlovich
Amichai
A
Department of Membrane and Ultrastructure Research, The Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Rottem
Shlomo
S
D001422:Bacterial Adhesion / Q000502:physiology*; D024061:Collagen Type III / Q000378:metabolism; D024141:Collagen Type IV / Q000378:metabolism; D024062:Collagen Type V / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D006367:HeLa Cells; D006493:Heparin / Q000378:metabolism; D006801:Humans; D007797:Laminin / Q000378:metabolism; D018613:Microscopy, Confocal; D016858:Mycoplasma fermentans / Q000378:metabolism* / Q000502:physiology; D010958:Plasminogen / Q000378:metabolism; D011485:Protein Binding
D024061:Collagen Type III; D024141:Collagen Type IV; D024062:Collagen Type V; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D007797:Laminin; D010958:Plasminogen; D006493:Heparin