title: Long polymeric tips of atomic force microscopy for large biological samples.
abstract: We show a new atomic force microscopy technique for obtaining high-resolution topographic images of large bio-samples. To obtain high-resolution topographic images for the samples, we fabricated a long polymeric tip with a small protrusion using two-photon adsorbed photo-polymerization techniques. The obtained tip length was over 50 microm, and the tip was used directly to visualize COS-1 and 293 cells. Compared with commercial tips, the long tip made it easier to obtain topographic images of the large cells. In the magnified topographic images, the sub-100-nm resolution was confirmed with the long tips. This long probe tip is expected to broaden large sample-related studies and applications in the future.
authors.affiliation: School of Bionics, Tokyo University of Technology, Katakura, Hachioji, Tokyo 192-0982, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Muramatsu
authors.forename: Yuji
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Atsushi
authors.initials: A
authors.lastname: Sato
authors.forename: Shizuo
authors.initials: S
authors.lastname: Enomoto
authors.forename: Woo-Sik
authors.initials: WS
authors.lastname: Kim
authors.forename: Sang-Mok
authors.initials: SM
authors.lastname: Chang
authors.forename: Jong Min
authors.initials: JM
authors.lastname: Kim
mesh_terms: D000818:Animals; D019556:COS Cells; D002460:Cell Line; D002522:Chlorocebus aethiops; D004867:Equipment Design; D006801:Humans; D007668:Kidney / Q000166:cytology*; D018625:Microscopy, Atomic Force / Q000295:instrumentation* / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D012117:Resins, Synthetic / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D011108:Polymers; D012117:Resins, Synthetic