title: Pathogenesis of cytomegalovirus-associated labyrinthitis in a guinea pig model.
abstract: Cytomegalovirus infects fetuses through the placenta, resulting in various congenital disorders in newborns, including hearing loss. We developed a monoclonal antibody to guinea pig cytomegalovirus (GPCMV) that was available for immunohistochemistry, and investigated the expression of the GPCMV antigen in animal models of direct and congenital infections. Injection of GPCMV, directly to the inner ear, increased the sound pressure level and resulted in labyrinthitis with severe inflammation. Immunohistochemistry detected GPCMV-infected cells mainly in the scala tympani, scala vestibule and spinal ganglion, but rarely in the cochlear duct. Injection of GPCMV to 5-week pregnant guinea pigs resulted in severe labyrinthitis in fetuses. Immunohistochemistry detected GPCMV-infected cells in the perilymph area and spinal ganglion, but not in the endolymph area, including hair cells. These data suggest that the virus spreads via the perilymph and neural routes in the inner ear of both models of direct and congenital infections.
authors.affiliation: Department of Pathology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo 162-8640, Japan. katano@nih.go.jp
authors.forename: Harutaka
authors.initials: H
authors.lastname: Katano
authors.forename: Yuko
authors.initials: Y
authors.lastname: Sato
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Tsutsui
authors.forename: Tetsutaro
authors.initials: T
authors.lastname: Sata
authors.forename: Akihiko
authors.initials: A
authors.lastname: Maeda
authors.forename: Naoki
authors.initials: N
authors.lastname: Nozawa
authors.forename: Naoki
authors.initials: N
authors.lastname: Inoue
authors.forename: Yasuya
authors.initials: Y
authors.lastname: Nomura
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kurata
mesh_terms: D000818:Animals; D003053:Cochlear Duct / Q000821:virology; D004195:Disease Models, Animal; D004710:Endolymph / Q000821:virology; D005727:Ganglia, Spinal / Q000821:virology; D006168:Guinea Pigs; D007150:Immunohistochemistry; D007249:Inflammation / Q000473:pathology; D007762:Labyrinthitis / Q000473:pathology / Q000821:virology*; D010498:Perilymph / Q000821:virology; D019211:Roseolovirus / Q000502:physiology*; D019349:Roseolovirus Infections / Q000473:pathology / Q000821:virology*; D012533:Scala Tympani / Q000821:virology