Transcription profiling of adult and fetal human neuroprogenitors identifies divergent paths to maintain the neuroprogenitor cell state.
Global gene expression profiling was performed using RNA from adult human hippocampus-derived neuroprogenitor cells (NPCs) and multipotent frontal cortical fetal NPCs compared with adult human mesenchymal stem cells (hMSCs) as a multipotent adult stem cell control, and adult human hippocampal tissue, to define a gene expression pattern that is specific for human NPCs. The results were compared with data from various databases. Hierarchical cluster analysis of all neuroectodermal cell/tissue types revealed a strong relationship of adult hippocampal NPCs with various white matter tissues, whereas fetal NPCs strongly correlate with fetal brain tissue. However, adult and fetal NPCs share the expression of a variety of genes known to be related to signal transduction, cell metabolism and neuroectodermal tissue. In contrast, adult NPCs and hMSCs overlap in the expression of genes mainly involved in extracellular matrix biology. We present for the first time a detailed transcriptome analysis of human adult NPCs suggesting a relationship between hippocampal NPCs and white matter-derived precursor cells. We further provide a framework for standardized comparative gene expression analysis of human brain-derived NPCs with other stem cell populations or differentiated tissues. Disclosure of potential conflicts of interest is found at the end of this article.
Maisel
Martina
M
Department of Neurology, Technical University of Dresden, Dresden, Germany.
Herr
Alexander
A
Milosevic
Javorina
J
Hermann
Andreas
A
Habisch
Hans-Jörg
HJ
Schwarz
Sigrid
S
Kirsch
Matthias
M
Antoniadis
Gregor
G
Brenner
Rolf
R
Hallmeyer-Elgner
Susanne
S
Lerche
Holger
H
Schwarz
Johannes
J
Storch
Alexander
A
D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D016000:Cluster Analysis; D005260:Female; D005333:Fetus / Q000166:cytology* / Q000378:metabolism*; D020869:Gene Expression Profiling; D006624:Hippocampus / Q000378:metabolism; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D008648:Mesoderm / Q000166:cytology / Q000378:metabolism; D009474:Neurons / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*; D014158:Transcription, Genetic
D015415:Biomarkers