title: Molecular characterization and classification of a clip domain containing peptidase from the ectoparasite Lepeophtheirus salmonis (Copepoda, Crustacea).
abstract: Clip domain containing serine peptidases (CSPs) include one or more N-terminal clip domain(s) and a C-terminal serine peptidase domain that shares traits with both chymotrypsin and trypsin. CSPs are found in arthropods and are involved in embryonic patterning, immune responses and blood clotting. Among crustaceans only one CSP, which activates prophenoloxidase in crayfish, have previously been reported. We here present LsCSP1, the first CSP found in copepods. LsCSP1 is expressed in the subcuticular tissue and the transcription appears to be upregulated during development. In conjunction with previous studies of CSPs, this study suggests that LsCSP1 may play a role in the immune responses of L. salmonis. Phylogenetic and structural analyses indicate that the CSPs and catalytically inactive CSP homologs (CSPHs) constitute a monophyletic lineage.
authors.affiliation: Institute of Marine Research, Postbox 1870, N-5817 Bergen, Norway. rasmus.skern@imr.no
authors.forename: Rasmus
authors.initials: R
authors.lastname: Skern-Mauritzen
authors.forename: Petter
authors.initials: P
authors.lastname: Frost
authors.forename: Lars A
authors.initials: LA
authors.lastname: Hamre
authors.forename: Heidi
authors.initials: H
authors.lastname: Kongshaug
authors.forename: Frank
authors.initials: F
authors.lastname: Nilsen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D020134:Catalytic Domain / Q000235:genetics; D033342:Copepoda / Q000201:enzymology / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid
chemical_list: D010447:Peptide Hydrolases