Antimicrobial peptide modulation in a differentiated reconstructed gingival epithelium.
Gingival innate immunity has been studied by using biopsies and normal or transformed epithelial cell monolayers. To overcome individual biological variabilities and as a physiological alternative, we have proposed using a reconstructed tissue equivalent. In this study, we investigated the functionality and the stage of differentiation of a reconstructed human gingival epithelium. We also characterized this epithelium at the molecular level to investigate its differentiation stage compared with native human gingival epithelium. The expression levels and localization of markers related to proteins and lipids of well-differentiated stratified epithelium, such as cytokeratins, cornified envelope proteins and enzymes, or to factors in lipid synthesis and trafficking were examined. Immunohistochemistry revealed similar localization patterns in both types of epithelia and mRNA quantification showed a close resemblance of their expression profiles. We further revealed that, like native gingiva, reconstructed gingival epithelium was able to respond to pro-inflammatory or lipopolysaccharide stimuli by producing antimicrobial peptides hbetaD-2, hbetaD-3 or LL-37. Finally, we demonstrated that reconstructed human gingival epithelium, as a model, was good enough to be proposed as a functional equivalent for native human gingival epithelium in order to study the regulation of gingival innate immunity against periodontal infections.
Peyret-Lacombe
A
A
Laboratoire de Biologie Cellulaire Cutanée/IRPF Hotel Dieu, 2 Rue Viguerie, 31025 Toulouse, France.
Duplan
H
H
Watts
M
M
Charveron
M
M
Brunel
G
G
D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism*; D005786:Gene Expression Regulation; D005881:Gingiva / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D007633:Keratins / Q000378:metabolism; D007700:Kinetics; D050356:Lipid Metabolism; D008565:Membrane Proteins / Q000378:metabolism; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D023822:Tissue Engineering
D023181:Antimicrobial Cationic Peptides; D015415:Biomarkers; D008565:Membrane Proteins; D012333:RNA, Messenger; D007633:Keratins