Molecular cloning, mapping, and expression analysis of the EIF4A2 gene in pig.
A full-length cDNA clone encoding the eukaryotic translation initiation factor 4A, isoform 2 (EIF4A2), was cloned from the fetal skeletal cDNA library from the pig (Sus scrofa). EIF4A2 is a highly conserved gene for one of the protein-synthesis initiation factors involved in the binding of mRNA to the ribosome. Based on this cDNA sequence, the deduced protein of 407 amino acids contains the characteristic motifs shared by the DEAD-box supergene family. The genomic nucleotide sequence of this gene was determined and a single nucleotide polymorphism located in the 5' untranslated region was genotyped. The porcine EIF4A2 was expressed in all tissues examined but in variable amounts. The EIF4A2 expression level in muscle was upregulated through embryonic and neonatal development until adult, suggesting that porcine EIF4A2 was possibly involved in translation regulation of other muscle-related genes in muscle formation and development. In addition, we mapped porcine EIF4A2 to q4.1 of SSC13, in agreement with comparative mapping data.
Wang
Heng
H
Key Laboratory of Animal Genetics, Breeding, and Reproduction of Ministry of Education of China, Huazhong Agricultural University, Wuhan 430070, PR China.
Wang
Huanling
H
Zhu
Zhengmao
Z
Yang
Shulin
S
Li
Kui
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D033481:Chromosomes, Mammalian; D003001:Cloning, Molecular; D039601:Eukaryotic Initiation Factor-4A / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D016678:Genome; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D034421:Sus scrofa / Q000235:genetics* / Q000502:physiology
D039601:Eukaryotic Initiation Factor-4A