Quantitative assessment of the tetracycline resistance gene pool in cheese samples by real-time TaqMan PCR.
A TaqMan real-time PCR assay was developed to quantify the tetS gene pool present in retail cheeses. This protocol offers a rapid, specific, sensitive, and culture-independent method for assessing antibiotic resistance genes in food samples rich in fats and proteins.
Manuzon
Michele Y
MY
Department of Food Science and Technology, The Ohio State University, Parker FST Building, 2015 Fyffe Ct., Columbus, OH 43210-1007, USA.
Hanna
Scott E
SE
Luo
Hongliang
H
Yu
Zhongtang
Z
Harper
W James
WJ
Wang
Hua H
HH
D001426:Bacterial Proteins / Q000235:genetics*; D002611:Cheese / Q000382:microbiology*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis* / Q000302:isolation & purification; D026901:Membrane Transport Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D019914:Taq Polymerase; D013753:Tetracycline Resistance / Q000235:genetics*
D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D026901:Membrane Transport Proteins; D019914:Taq Polymerase