Cysteine-674 of the sarco/endoplasmic reticulum calcium ATPase is required for the inhibition of cell migration by nitric oxide.
OBJECTIVE
Nitric oxide inhibits smooth muscle cell migration after arterial injury, but the detailed mechanism is not fully understood. The sarco/endoplasmic reticulum calcium ATPase (SERCA) lowers cell Ca2+ by increasing intracellular Ca2+ uptake and inhibiting extracellular Ca2+ influx. Our previous studies showed that NO causes cyclic GMP-independent arterial relaxation by increasing SERCA activity by inducing reversible S-glutathiolation at cysteine-674. Because Ca2+ is an important second messenger for cell migration, we hypothesized that NO also inhibits cell migration through redox regulation of SERCA activity via cysteine-674.


RESULTS
To test our hypothesis, overexpression of either wild type (WT) or mutant SERCA in which cysteine-674 was mutated to serine was accomplished by stable transfection of HEK 293 or adenoviral expression in rat aortic smooth muscle cells (VSMCs). In the cell models expressing mutant SERCA, biotinylated-iodoacetamide (BIAM) and biotinylated-glutathione labeling of SERCA was decreased, and NO failed to increase SERCA activity or decrease Ca2+ influx, thus validating that the expression of mutant SERCA prevents its redox-dependent activation. In the absence of NO, fetal bovine serum stimulated migration of both cell types expressing WT or C674S SERCA at similar rates. The NO donor S-nitrosopenicillamine inhibited migration of cells with WT SERCA, but had no effect on the migration of either HEK cells or VSMCs with C674S SERCA. The same result was obtained in VSMCs in which endogenous NO was produced by iNOS induced by interleukin (IL)-1beta. Blocking cyclic GMP did not prevent the inhibition of migration by NO.


CONCLUSIONS
In cells overexpressing SERCA, the cyclic GMP-independent, redox regulation of SERCA cysteine-674 is required for the inhibition of cell migration by both exogenous and endogenously generated NO.
Ying
Jia
J
Vascular Biology Unit, Boston University School of Medicine, Boston, MA 02118, USA.
Tong
XiaoYong
X
Pimentel
David R
DR
Weisbrod
Robert M
RM
Trucillo
Mario P
MP
Adachi
Takeshi
T
Cohen
Richard A
RA
D000595:Amino Acid Sequence; D000818:Animals; D001011:Aorta / Q000166:cytology; D002118:Calcium / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D006152:Cyclic GMP / Q000378:metabolism; D003545:Cysteine; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D009154:Mutation; D032389:Myocytes, Smooth Muscle / Q000502:physiology*; D009569:Nitric Oxide / Q000378:metabolism / Q000494:pharmacology*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000235:genetics* / Q000378:metabolism*; D014162:Transfection
D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; C506946:ATP2A2 protein, human; D005978:Glutathione; D006152:Cyclic GMP; D003545:Cysteine; D002118:Calcium