[Effect of hydrogen concentration on the hydrogenotrophic methanogenic community structure studied by T-RELP analysis of 16S rRNA gene amplicons].
Analysis of length polymorphism of 16S rRNA genes terminal restriction fragments (T-RFLP analysis) was used to monitor the changes in the composition of the population of methanogens in enrichment cultures under high and low hydrogen concentrations. Hydrogen concentration was shown to determine the structure of a methanogenic community. High hydrogen concentration probably favors the hydrogen- and acetate-utilizing representatives of Methanosarcinaceae, while a more diverse methanogenic community is favored by low hydrogen concentrations.
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D001105:Archaea / Q000145:classification / Q000302:isolation & purification* / Q000502:physiology; D003470:Culture Media; D017753:Ecosystem; D004784:Environmental Monitoring / Q000379:methods; D019605:Euryarchaeota / Q000145:classification / Q000302:isolation & purification* / Q000502:physiology; D006859:Hydrogen; D008697:Methane / Q000096:biosynthesis; D012275:Oryza; D012150:Polymorphism, Restriction Fragment Length; D019642:RNA, Archaeal / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013045:Species Specificity
D003470:Culture Media; D019642:RNA, Archaeal; D012336:RNA, Ribosomal, 16S; D006859:Hydrogen; D008697:Methane