Immunogenicity of empty capsids of porcine circovius type 2 produced in insect cells.
Porcine circovirus type 2 (PCV2), a single-stranded DNA virus, is associated with postweaning multisystemic wasting syndrome (PMWS). ORF2 protein (capsid) of PCV2 was recently demonstrated to be a major immunogenable to induce protection in pigs with a prime-boost protocol. In this study, the ORF2 gene of PCV2 was expressed in insect cells. The product self-assembled into particles that were structurally and antigenically indistinguishable from regular PCV2 capsids. To evaluated the immunogenicity of these virus-like particles, PCV2-free piglets were vaccinated with the crude lysate from recombinant baculovirus (Ac.ORF2)-infected insect cells, at doses of 0.1 ml (10(6) cells), 0.5 mL (5 x 10(6) cells) or 1.0 ml (10(7) cells). The immune response was monitored by an indirect enzyme-linked immunosorbent assay (ELISA) for PCV2 antibody and lymphocyte proliferation assay. The ELISA results indicated that primary immune response was elicited with 0.5 ml or 1.0 ml of crude lysate from Ac.ORF2. After boost immunization, relatively higher levels of PCV2 antibody were elicited in 0.5-ml or 1.0-ml vaccinated groups, compared to the 0.1-ml group. In addition, higher PCV2 specific lymphocyte proliferation response was developed in piglets vaccinated with 0.5 ml or 1.0 ml of crude lysate, especially in those vaccinated with with 1.0 ml of crude lysate. Thus, the expressed ORF2 protein has significant potential as a subunit vaccine against PCV2 infection.
Fan
H
H
Laboratory of Animal Virology, College of Veterinary Medicine, and The National Key Laboratory ofAgricultural Microbiology, Huazhong Agricultural University, Wuhan, China. fanhy@webmail.hzau.edu.cn
Ju
C
C
Tong
T
T
Huang
H
H
Lv
J
J
Chen
H
H
D000818:Animals; D016367:Baculoviridae; D002213:Capsid / Q000276:immunology*; D002460:Cell Line; D017925:Circovirus / Q000276:immunology*; D015967:Gene Expression Regulation, Viral; D007313:Insecta / Q000166:cytology*; D014765:Viral Vaccines / Q000276:immunology
D014765:Viral Vaccines