A solid-phase extraction method for high-performance liquid chromatographic determination of salvianolic acid B in rabbit plasma: application to pharmacokinetic study.
A sensitive solid-phase extraction/high-performance liquid chromatographic method with ultraviolet detection was established for the analysis of salvianolic acid B in rabbit plasma. The analyte was separated on a reversed-phase column with trifluoroacetic acid-methanol-acetonitrile (70:10:20, v/v/v) as mobile phase at a flow rate of 1 mL/min, and ultraviolet detection at 315 nm. The calibration curve for salvianolic acid B was linear over the range 35-1400 microg/L with coefficients of correlation >0.999. The inter-day and intra-day precisions of analysis were <15%, and assay accuracy ranged from 95.3 to 109.1%. This method is suitable for determining salvianolic acid B in plasma and thus investigating the pharmacokinetics of salvianolic acid B.
Ma
Yueming
Y
College of Chinese Material Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, People's Republic of China. mayueming_117@hotmail.com
Wang
Tianming
T
D000818:Animals; D001572:Benzofurans / Q000097:blood* / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D008297:Male; D011817:Rabbits; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*; D053630:Depsides
D001572:Benzofurans; C076944:salvianolic acid B; D053630:Depsides