title: Circulating monocyte oxidative activity is increased in patients with type 2 diabetes and erectile dysfunction.
abstract: OBJECTIVE
  We investigated the relationship between oxidative stress and diabetic erectile dysfunction.
  
  
  METHODS
  A total of 23 patients with a mean +/- SD age of 56.7 +/- 5.6 years, a history of type 2 diabetes for 10.0 +/- 8.3 years and erectile dysfunction, as tested by the International Index of Erectile Function questionnaire, but without vascular and neurological complications, and 15 age matched patients with diabetes without erectile dysfunction were recruited. Circulating monocyte oxidative activity by cytofluorometry, and endothelin-1, intercellular adhesion molecule-1, plasminogen activator inhibitor-1 by enzyme linked immunosorbent assay were evaluated in all patients in the study.
  
  
  RESULTS
  Monocyte free radical production, and total and low density lipoprotein cholesterol were higher in patients with than in those without erectile dysfunction (p <0.03, <0.02 and <0.05, respectively). In all patients the International Index of Erectile Function score inversely correlated with low density lipoprotein (p <0.05), while in patients with erectile dysfunction it negatively correlated with age (p <0.03), body mass index (p <0.02), endothelin-1 (p <0.02) and intercellular adhesion molecule-1 (p <0.05). Endothelin-1, intercellular adhesion molecule-1 and plasminogen activator inhibitor-1 were not different in patients with diabetes with and without erectile dysfunction.
  
  
  CONCLUSIONS
  In men with type 2 diabetes who have erectile dysfunction but are asymptomatic for cardiovascular disease oxidative activation of monocytes is increased and it is related to other risk factors of endothelial dysfunction.
authors.affiliation: Department of Clinical Sciences, University La Sapienza, Rome, Italy. susanna.morano@uniroma1.it
authors.forename: Susanna
authors.initials: S
authors.lastname: Morano
authors.forename: Alessandra
authors.initials: A
authors.lastname: Gatti
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Mandosi
authors.forename: Claudio
authors.initials: C
authors.lastname: Tiberti
authors.forename: Mara
authors.initials: M
authors.lastname: Fallarino
authors.forename: Rosalba
authors.initials: R
authors.lastname: Cipriani
authors.forename: Barbara
authors.initials: B
authors.lastname: Buchetti
authors.forename: Loredana
authors.initials: L
authors.lastname: Gandini
authors.forename: Paolo
authors.initials: P
authors.lastname: Sgrò
authors.forename: Emmanuele Angelo
authors.initials: EA
authors.lastname: Jannini
authors.forename: Luisa
authors.initials: L
authors.lastname: Lenti
authors.forename: Andrea
authors.initials: A
authors.lastname: Lenzi
mesh_terms: D048909:Diabetes Complications / Q000097:blood / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000378:metabolism*; D019332:Endothelin-1 / Q000097:blood; D004730:Endothelium, Vascular; D007172:Erectile Dysfunction / Q000097:blood / Q000378:metabolism*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D019332:Endothelin-1; D017395:Plasminogen Activator Inhibitor 1; D017382:Reactive Oxygen Species; D018799:Intercellular Adhesion Molecule-1