title: [The effect of ligustrazine on cells proliferation in cortex and striatum after focal cerebral ischemia in adult rats].
abstract: OBJECTIVE
  To explore the effect of Ligustrazine on cells proliferation in cortex and striatum after focal cerebral ischemia in adult rats.
  
  
  METHODS
  Focal cerebral ischemia was induced by left middle cerebral artery occlusion (MCAO) with suture method. Two hours later, injection of Ligustrazine (80 mg/kg, 1 time/d) was performed peritoneally. Four hours after the ischemia, 5-bromodeoxyuridine (BrdU) (50 mg/kg, 1 time/d) was injected peritoneally. At 7d, 14d and 21d after ischemia, BrdU-Labeled cells in the cortex and striatum were observed by immunohistochemical staining.
  
  
  RESULTS
  In ischemia model group, at 7 day, BrdU-labeled cells were observed in the ipsilateral cortex and striatum. With the prolongation of ischemia, the number of BrdU-labeled cells increased, reached the peak at 21d. In Ligustrazine group, BrdU-labeded cells were observed with an intense distribution in ischemic penumbra of the cortex and striatum. With the prolongation of ischemia, the number of BrdU-labeled cells increased significantly at 14d, and reached the peak at 21d. The numbers of BrdU-labeled cells at 7d, 14d and 21d were more than those in ischemia model group respectively.
  
  
  CONCLUSIONS
  Ligustrazine increase the proliferated cells in the cortex and striatum after focal cerebral ischemia in adult rats. The results suggest that it may be useful for promoting self-repair after ischemia.
authors.affiliation: Research Center for Neuroscience, Department of Human Anatomy and Histological Embryology, Xi'an Jiaotong University, Xi'an 710061, China. qiufen6688@mail.xjtu.edu.cn
authors.forename: Fen
authors.initials: F
authors.lastname: Qiu
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Peng-Bo
authors.initials: PB
authors.lastname: Zhang
authors.forename: Ying-Fang
authors.initials: YF
authors.lastname: Tian
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Zhao
authors.forename: Qian-Yan
authors.initials: QY
authors.lastname: Kang
authors.forename: Cun-Fang
authors.initials: CF
authors.lastname: Qi
authors.forename: Xin-Lin
authors.initials: XL
authors.lastname: Chen
mesh_terms: D000818:Animals; D019661:Apiaceae / Q000737:chemistry; D002545:Brain Ischemia / Q000188:drug therapy / Q000473:pathology*; D002452:Cell Count; D049109:Cell Proliferation / Q000187:drug effects*; D002540:Cerebral Cortex / Q000473:pathology; D003342:Corpus Striatum / Q000473:pathology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D020244:Infarction, Middle Cerebral Artery / Q000188:drug therapy / Q000473:pathology*; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry; D011719:Pyrazines / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004365:Drugs, Chinese Herbal; D011719:Pyrazines; C017953:tetramethylpyrazine