[The influence of Solanum lyratum Thunb extract on apoptosis and the expression of fas/fasL genes in Hela cells].
OBJECTIVE
To explore the effects of Solanum lyratum Thunb (SL) extract on the apoptosis and the expression of fas and fasL genes in Hela cells.


METHODS
The proliferation inhibitory rate was evaluated by MTF assay. Induction of cell apoptosis rate was determined by flow cytometry. The expression of Fas protein was detected by two-step immunhistochemical staining. The expression of fas and fasL mRNA was detected by semi-quantitive RT-PCR.


RESULTS
SL extract displayed strong proliferation inhibitory effect in a dose-and-time-dependent manner against Hela cell. The rate of apoptosis was increased obviously. The expression of fas mRNA and protein was increased significantly, and fasL mRNA was decreased markedly.


CONCLUSIONS
SL can induce apoptosis by up-regulating expression of fas and fasL genes, and inhibit the development of Hela cells.
Wei
Xing
X
Department of Biochemistry and Molecular Biology, Youjiang Medical College for Nationalities, Baise 533000, China.
Li
Chao-Gan
CG
Nong
Song
S
Zhu
Xiao-Ying
XY
Huang
Xiao-Min
XM
D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D053222:Fas Ligand Protein / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis; D019657:Solanaceae / Q000737:chemistry*; D019014:fas Receptor / Q000096:biosynthesis* / Q000235:genetics
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D053222:Fas Ligand Protein; D012333:RNA, Messenger; D019014:fas Receptor