title: Morphological alterations in oxidative muscles and mitochondrial structure associated with equine atypical myopathy.
abstract: BACKGROUND
  There is a lack of well documented studies about muscular lesions in equine atypical myopathy (EAM).
  
  
  OBJECTIVE
  To characterise morphopathological changes of striated muscles and myocardium, to progress understanding of this disease.
  
  
  METHODS
  Thirty-two horses age 0.5-7 years kept on pasture were referred for a sudden ataxia/myoglobinuria syndrome. Clinical examination (stiffness, muscle pain, muscle fasciculations, abnormal gait, recumbency, myoglobinuria, tachycardia, sweating) and plasma CPK, LDH and AST levels were consistent with extensive myonecrosis and, together with anamnestic data, with so-called 'equine atypical myopathy' (EAM), a disease of unknown aetiology reported since 1939. Macroscopic and microscopic (histology, histoenzymology, ultrastructure) lesions were evaluated.
  
  
  RESULTS
  Necropsic examination revealed large areas of muscle necrosis, the extent and severity of which varied between cases and muscles, but which were clearly more constant and severe in respiratory and postural muscles and in the myocardium. Histology highlighted a multifocal and monophasic process compatible with Zenker degeneration/necrosis that mostly and segmentally affected type 1 fibres. Histochemical evaluation revealed a weak and disorganised pattern of NADH tetrazolium reductase staining, the absence of calcium salts precipitates and a dramatic accumulation of lipid droplets. Ultrastructural examination often revealed fibres of which the sole modifications were altered mitochondria and sarcoplasmic lipidosis.
  
  
  CONCLUSIONS
  Taken together, the data suggest that a primary alteration of mitochondria should be considered, although secondary mitochondrial abnormalities have yet to be ruled out.
  
  
  CONCLUSIONS
  The morphological features gathered here reveal that EAM shares most of the characteristics of toxic myopathies.
authors.affiliation: Department of Pathology, Faculty of Veterinary Medicine, University of Liège, Sart Tilman B43, B-4000 Liège, Belgium.
authors.forename: D
authors.initials: D
authors.lastname: Cassart
authors.forename: E
authors.initials: E
authors.lastname: Baise
authors.forename: Y
authors.initials: Y
authors.lastname: Cherel
authors.forename: C
authors.initials: C
authors.lastname: Delguste
authors.forename: N
authors.initials: N
authors.lastname: Antoine
authors.forename: D
authors.initials: D
authors.lastname: Votion
authors.forename: H
authors.initials: H
authors.lastname: Amory
authors.forename: F
authors.initials: F
authors.lastname: Rollin
authors.forename: A
authors.initials: A
authors.lastname: Linden
authors.forename: F
authors.initials: F
authors.lastname: Coignoul
authors.forename: D
authors.initials: D
authors.lastname: Desmecht
mesh_terms: D000818:Animals; D005260:Female; D006734:Horse Diseases / Q000378:metabolism* / Q000473:pathology; D006736:Horses; D008297:Male; D008929:Mitochondria, Heart / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D008931:Mitochondria, Muscle / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D018482:Muscle, Skeletal / Q000378:metabolism*; D009336:Necrosis / Q000662:veterinary; D010084:Oxidation-Reduction; D012206:Rhabdomyolysis / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*