Marinobacterium halophilum sp. nov., a marine bacterium isolated from the Yellow Sea.
A moderately halophilic, aerobic, Gram-negative bacterium was isolated from a tidal flat area of Dae-Chun, Chung-Nam, Korea. The strain, designated mano11(T), comprised rod-shaped cells that were motile by means of polar flagella. It grew with 3-12 % NaCl and at 4-37 degrees C and pH 5.3-9.3. The predominant menaquinone present in this strain was Q-8 [corrected] and diaminopimelic acid was not found in the cell-wall peptidoglycan. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain mano11(T) belongs to the genus Marinobacterium. Strain mano11(T) exhibited 92.8-98.3 % 16S rRNA gene sequence similarity when compared with the type strains of three other species of the genus Marinobacterium. DNA-DNA hybridization between strain mano11(T) and Marinobacterium georgiense DSM 11526(T), its closest relative in terms of 16S rRNA gene sequence similarity, was 13 %. On the basis of the phenotypic, genetic and phylogenetic data, strain mano11(T) represents a novel species of the genus Marinobacterium, for which the name Marinobacterium halophilum sp. nov. is proposed. The type strain is mano11(T) (=KCTC 12240(T)=DSM 17586(T)).
Chang
Ho-Won
HW
Department of Biology, Chungnam National University, Daejeon 306-764, Korea.|Biological Resource Center, KRIBB, Daejeon 305-806, Korea.
Nam
Young-Do
YD
University of Science and Technology, Daejeon 305-333, Korea.|Biological Resource Center, KRIBB, Daejeon 305-806, Korea.
Kwon
Hyuk-Yong
HY
Biological Resource Center, KRIBB, Daejeon 305-806, Korea.
Park
Ja Ryeong
JR
Biological Resource Center, KRIBB, Daejeon 305-806, Korea.
Lee
Jung-Sook
JS
Biological Resource Center, KRIBB, Daejeon 305-806, Korea.
Yoon
Jung-Hoon
JH
21 C Frontier Microbial Genomics and Applications Center, KRIBB, Daejeon 305-806, Korea.
An
Kwang-Guk
KG
Department of Biology, Chungnam National University, Daejeon 306-764, Korea.
Bae
Jin-Woo
JW
Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660-701, Korea.|University of Science and Technology, Daejeon 305-333, Korea.|Biological Resource Center, KRIBB, Daejeon 305-806, Korea.
D034241:Alteromonadaceae / Q000145:classification* / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D007723:Korea; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S