Contrasting geographic distribution profiles of the herpes simplex virus type 1 BgOL and BgKL variants in Japan suggest dispersion and replacement.
Thelifelong latent infection-reactivation mode of infection of herpes simplex virus type 1 (HSV-1) transmitted by close contact has allowed a diversity of restriction fragment length polymorphism (RFLP) variations to accumulate in human populations. Whether and how the variants of the HSV-1 that is ubiquitous worldwide spread to different human populations is not clear. In our previous study the geographically gradient distribution of the HSV-1 BgK(L) variant, which is a good marker for the BgK(L):SaCFJ(M):SaGH(M):SaD/E(L):KpM(S) variant, suggested that BgK(L) dispersed geographically. Southern hybridization analyses showed that in BgK(L) the BglII cleavage site between the BglII K and small "Q/#13" fragments is lost, the SalI cleavage sites between the SalI J and C and between SalI F and J fragments are lost, and the SalI E fragment is abnormally large (SaE(L) variation). The RFLP and geographic distribution of one more HSV-1 RFLP variant, BgO(L), were comparatively analyzed. The BglII cleavage site between the BglII O and Q/#13 fragments is lost in BgOL. BgO(L) clinical isolates were not associated with any of the SaCFJ(M), SaE(L), SaGH(M), or KpM(S) variations, whereas one-fourth of the non-BgO(L):non-BgK(L) isolates was associated with SaCFJ(M) and SaGH(M), indicating that BgK(L) and BgO(L) are distant in terms of diversification. BgO(L) is distributed highly in the northeastern region and the southwestern island of Kyushu but is rare between the two regions in Japan, in a remarkable contrast to BgK(L). These are the first epidemiologic data to show contrasting geographic distribution profiles of two HSV-1 variants and suggest the gradual dispersion and replacement of HSV-1 variants.
Eda
Hiroyuki
H
Herpesvirus Laboratory, Department of Virus I, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan.
Ozawa
Shigeru
S
Yoshino
Kamesaburo
K
Yanagi
Kazuo
K
D001426:Bacterial Proteins / Q000378:metabolism*; D001483:Base Sequence; D015139:Blotting, Southern; D004279:DNA, Viral / Q000032:analysis; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D014644:Genetic Variation; D006561:Herpes Simplex / Q000453:epidemiology* / Q000821:virology; D018259:Herpesvirus 1, Human / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence
D001426:Bacterial Proteins; D004279:DNA, Viral; C024404:BglII endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific