[Role of AcSDKP on collagen synthesis and degradation in cultured rat cardiac fibroblast].
OBJECTIVE
To investigate the role of AcSDKP on collagen synthesis and degradation in cultured rat cardiac fibroblasts.


METHODS
Neonatal rat cardiac fibroblasts were isolated and stimulated by PDGF. The cell proliferation was observed by (3)H-TdR incorporation assay. The synthesis of collagen was measured by (3)H-proline incorporation assay. The expression of type I and type III collagen and MMP-1 protein were measured by Western blot. The MMP-2 and MMP-9 activity was evaluated with zymography assay.


RESULTS
PDGF stimulated cardiac fibroblasts proliferation with increased collagen synthesis and type I and type III collagen protein expressions as well as MMP-2 and MMP-9 activities and MMP-1 expression. AcSDKP inhibited cardiac fibroblasts proliferation induced by PDGF and reduced collagen synthesis and type I and type III collagen protein expression. AcSDKP also further up-regulated MMP-2 and MMP-9 activities and MMP-1 expression in cardiac fibroblasts induced by PDGF.


CONCLUSIONS
AcSDKP inhibited proliferation and collagen synthesis and up-regulated matrix metalloproteinases activity or expression induced by PDGF, which was possibly related with the effect of AcSDKP anti-fibrosis.
Yang
Fang
F
Experimental and Research Center, North China Coal Medical College, Tangshan 063000, China.
Zhu
Xi-ling
XL
Wang
Li-ping
LP
Song
Xu-dong
XD
Wang
Rui-min
RM
Li
Zhi-guo
ZG
Luo
Ling
L
Hu
Wan-mi
WM
Ma
Wen-dong
WD
Pei
Xin
X
Zhang
Li-juan
LJ
Li
Qi-jia
QJ
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism*; D009842:Oligopeptides / Q000502:physiology*; D010982:Platelet-Derived Growth Factor / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
D009842:Oligopeptides; D010982:Platelet-Derived Growth Factor; D003094:Collagen; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; C058504:goralatide