Bacterial counts in organic materials used as free-stall bedding following treatment with a commercial conditioner.
An acidic conditioner was tested in recycled manure and sawdust used as free-stall bedding to determine the effect on bacterial counts of common environmental mastitis pathogens. Free stalls were bedded with 10 kg of either kiln-dried sawdust or recycled manure per stall. All bedding was removed and fresh bedding was applied every 7 d. Approximately 1 kg of commercial bedding conditioner containing 93% sodium hydrosulfate (wt/wt) was spread evenly over bedding in the back one-third of each treated stall. Control bedding received no treatment. Cows were bedded on the same material for 3 consecutive weeks. After 3 wk on a bedding treatment, cows remained in the same pens and bedding treatments were changed between rows within pens in a switchback design. The addition of bedding conditioner to sawdust reduced gram-negative bacterial, coliform, Klebsiella spp., and streptococcal counts immediately after application and 1 d after application. Bacterial counts did not differ between treated and untreated sawdust on d 2 and 6 after the conditioner treatment. Each bacterial count measured was reduced in recycled manure immediately after application. Gram-negative bacterial and streptococcal counts were reduced in treated recycled manure compared with untreated recycled manure on d 1 after conditioner was applied. Bacterial counts did not differ between treated and untreated recycled manure bedding on d 2 and 6 after application in free stalls. The antibacterial activity of the conditioner was related to the pH of the bedding and was diminished by d 2 after application in both bedding types.
Hogan
J S
JS
Department of Animal Sciences, The Ohio State University, Ohio Agricultural Research and Development Center, Wooster 44691, USA. hogan.4@osu.edu
Wolf
S L
SL
Petersson-Wolfe
C S
CS
D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D002417:Cattle; D015169:Colony Count, Microbial; D004755:Enterobacteriaceae / Q000166:cytology; D005260:Female; D006090:Gram-Negative Bacteria / Q000166:cytology; D006799:Housing, Animal; D006863:Hydrogen-Ion Concentration; D007709:Klebsiella / Q000166:cytology; D008372:Manure / Q000382:microbiology; D008414:Mastitis, Bovine / Q000382:microbiology; D013291:Streptococcus / Q000166:cytology; D013431:Sulfates / Q000008:administration & dosage*; D014934:Wood / Q000382:microbiology
D000900:Anti-Bacterial Agents; D008372:Manure; D013431:Sulfates; C012036:sodium sulfate