title: Expression and prognostic significance of Stat5a and E-cadherin in extramammary Paget's disease.
abstract: BACKGROUND
  Stat5 has been shown to regulate the proliferation and inhibition of apoptosis in cancer cells. E-cadherin plays an important role in maintaining epithelial stability and is widely regarded as a prognostic marker in many types of human cancers. The expression of Stat5 has not been investigated in extramammary Paget's disease (EMPD).
  
  
  OBJECTIVE
  To study the expression of Stat5a and E-cadherin protein in EMPD and to evaluate the relationships between them.
  
  
  METHODS
  Thirty-six tissue samples from 34 cases with primary EMPD were subjected to immunohistochemical staining for Stat5a and E-cadherin.
  
  
  RESULTS
  Twenty-nine of 30 in situ EMPD specimens were positive for Stat5a expression, and the staining pattern for Stat5a was mainly nuclear in the majority of Paget's cells. All four invasive specimens and two metastatic lymph node specimens were negative for Stat5a. Twenty-six of 30 in situ EMPD specimens expressed E-cadherin in more than 50% of tumor cells, and three of 30 in situ EMPD specimens expressed E-cadherin in less than 50% of tumor cells. One in situ EMPD specimen and one invasive specimen expressed E-cadherin in less than 25% of tumor cells. Three invasive specimens and two metastatic lymph node specimens were negative, with occasional membranous staining for E-cadherin. There is a significant correlation between the expression of Stat5a and E-cadherin.
  
  
  CONCLUSIONS
  Our results showed that these proteins were found in in situ lesions, and it is speculated that these proteins may play some role in the prognosis/invasion of EMPD.
authors.affiliation: Department of Dermatology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Houjun
authors.initials: H
authors.lastname: Liu
authors.forename: Kazunori
authors.initials: K
authors.lastname: Urabe
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Uchi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Takeuchi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nakahara
authors.forename: Teruki
authors.initials: T
authors.lastname: Dainichi
authors.forename: Yating
authors.initials: Y
authors.lastname: Tu
authors.forename: Masutaka
authors.initials: M
authors.lastname: Furue
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Moroi
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015820:Cadherins / Q000378:metabolism*; D020405:Dermis / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008198:Lymph Nodes / Q000378:metabolism; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D010145:Paget Disease, Extramammary / Q000378:metabolism* / Q000473:pathology; D011379:Prognosis; D050799:STAT5 Transcription Factor / Q000378:metabolism*; D016037:Single-Blind Method; D013194:Staining and Labeling; D014018:Tissue Distribution; D025521:Tumor Suppressor Proteins
chemical_list: D015820:Cadherins; D050799:STAT5 Transcription Factor; C494092:STAT5A protein, human; D025521:Tumor Suppressor Proteins