title: PCR detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii in stool samples from Sydney, Australia.
abstract: This study investigated the presence of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii in stool samples from a patient population in Sydney, Australia. Stool samples were tested by microscopy and PCR. Five patients were found with E. histolytica infections, while E. dispar and E. moshkovskii were observed in 63 (70.8%) and 55 (61.8%) patients, respectively, by PCR. This is the first study in Australia using molecular techniques to determine the presence of E. histolytica, E. dispar, and E. moshkovskii.
authors.affiliation: Department of Microbiology, St. Vincent's Hospital, Sydney, Australia.
authors.forename: R
authors.initials: R
authors.lastname: Fotedar
authors.forename: D
authors.initials: D
authors.lastname: Stark
authors.forename: N
authors.initials: N
authors.lastname: Beebe
authors.forename: D
authors.initials: D
authors.lastname: Marriott
authors.forename: J
authors.initials: J
authors.lastname: Ellis
authors.forename: J
authors.initials: J
authors.lastname: Harkness
mesh_terms: D000328:Adult; D000818:Animals; D001315:Australia / Q000453:epidemiology; D016054:DNA, Protozoan / Q000032:analysis / Q000302:isolation & purification; D004404:Dysentery, Amebic / Q000453:epidemiology / Q000469:parasitology*; D004747:Entamoeba / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004748:Entamoeba histolytica / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004749:Entamoebiasis / Q000453:epidemiology / Q000469:parasitology*; D005243:Feces / Q000469:parasitology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D016054:DNA, Protozoan