title: Serum S100B indicates brain edema formation and predicts long-term neurological outcomes in rat transient middle cerebral artery occlusion model.
abstract: To assess the usefulness of serum S100B as a biomarker, the present study proceeded by observing serum S100B kinetics in a rat transient middle cerebral artery occlusion (MCAO) model, then assessed the correlation between serum S100B and both brain edema formation and neurological outcomes. Study results showed increases in serum S100B concentrations, peaking 48 h after MCAO. Brain water content in the ipsilateral hemisphere significantly increased from 24 h after MCAO, and reached peak value 72 h after MCAO. A single measurement of serum S100B 48 h after MCAO showed significant correlations with maximal extent of brain edema 72 h after MCAO. Furthermore, S100B concentrations 48 h after MCAO significantly correlated with infarct volumes. Neurological outcomes were estimated in a long-term study, where a gradual recovery was observed up to 168 h after MCAO. Serum S100B 48 h after MCAO was found to show higher correlation with neurological score 168 h after MCAO than those 48 h after MCAO. These findings suggest that serum S100B is an effective biomarker in predicting both extent of brain edema and long-term neurological outcomes in a rat transient MCAO model.
authors.affiliation: Medical Research Laboratories, Taisho Pharmaceutical Co. Ltd., 1-403 Yoshino-cho, Kita-ku, Saitama-shi, Saitama 331-9530, Japan. yu.tanaka@po.rd.taisho.co.jp
authors.forename: Yu
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Chie
authors.initials: C
authors.lastname: Koizumi
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Marumo
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Omura
authors.forename: Shigeru
authors.initials: S
authors.lastname: Yoshida
mesh_terms: D000818:Animals; D001929:Brain Edema / Q000175:diagnosis* / Q000209:etiology*; D004195:Disease Models, Animal; D007839:Functional Laterality; D020244:Infarction, Middle Cerebral Artery / Q000097:blood* / Q000150:complications* / Q000473:pathology; D008297:Male; D009414:Nerve Growth Factors / Q000097:blood*; D009460:Neurologic Examination; D011237:Predictive Value of Tests; D051381:Rats; D017208:Rats, Wistar; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000097:blood*; D012720:Severity of Illness Index; D013223:Statistics as Topic; D013997:Time Factors
chemical_list: D009414:Nerve Growth Factors; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; C579158:S100b protein, rat