title: Simplicispira limi sp. nov., isolated from activated sludge.
abstract: A Gram-negative, motile, rod-shaped bacterium, designated strain EMB325(T), was isolated from activated sludge that performed enhanced biological phosphorus removal in a sequencing batch reactor. The predominant fatty acids of strain EMB325(T) were summed feature 3 (C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH), C(16 : 0), C(18 : 1)omega7c and C(18 : 1)omega7c 11-methyl. The strain contained phosphatidylethanolamine and diphosphatidylglycerol as polar lipids. The G+C content of the genomic DNA was 63.3 mol%. The major quinone was Q-8. Phylogenetic analysis of 16S rRNA gene sequences showed that strain EMB325(T) formed a phyletic lineage with members of the genus Simplicispira and was most closely related to Simplicispira psychrophila LMG 5408(T) and Simplicispira metamorpha DSM 1837(T) with similarities of 98.1 and 97.9 %, respectively. Levels of DNA-DNA relatedness between strain EMB325(T) and S. psychrophila LMG 5408(T) and S. metamorpha DSM 1837(T) were 28 and 23 %, respectively. On the basis of chemotaxonomic data and molecular characteristics, strain EMB325(T) is considered to represent a novel species within the genus Simplicispira, for which the name Simplicispira limi sp. nov. is proposed. The type strain is EMB325(T) (=KCTC 12608(T)=DSM 17964(T)).
authors.affiliation: Division of Applied Life Science, EB-NCRC, PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Shipeng
authors.initials: S
authors.lastname: Lu
authors.affiliation: Division of Applied Life Science, EB-NCRC, PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Seung Hyun
authors.initials: SH
authors.lastname: Ryu
authors.affiliation: Division of Applied Life Science, EB-NCRC, PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Bok Sil
authors.initials: BS
authors.lastname: Chung
authors.affiliation: Division of Applied Life Science, EB-NCRC, PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Young Ryun
authors.initials: YR
authors.lastname: Chung
authors.affiliation: Division of Environmental Science and Ecological Engineering, Korea University, Seoul 136-701, Republic of Korea.
authors.forename: Woojun
authors.initials: W
authors.lastname: Park
authors.affiliation: Division of Applied Life Science, EB-NCRC, PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Che Ok
authors.initials: CO
authors.lastname: Jeon
mesh_terms: D015373:Bacterial Typing Techniques; D042621:Comamonadaceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012722:Sewage