title: Methamphetamine downregulates peroxiredoxins in rat pheochromocytoma cells.
abstract: Methamphetamine (METH) is an abusive psychostimulant that induces neuronal cell death/degeneration in experimental animals and humans. METH-induced apoptosis in rat pheochromocytoma cells was utilized to study the neurotoxic mechanism. During METH intoxication, we found that peroxiredoxins and thioredoxins/thioredoxin reductases (peroxiredoxin reducing systems) which are known to prevent oxidative stress and apoptosis were differentially downregulated and upregulated, respectively. We also found not only the free radicals but also the oxidative forms of peroxiredoxin and thioredoxin were increased, indicating the dysfunction of these enzymes. Thus, METH-induced differential regulation and oxidation of peroxiredoxins and thioredoxin may be an important mechanism for apoptosis.
authors.affiliation: Department of Life Science, Fu-Jen Catholic University, Taiwan, ROC.
authors.forename: Han-Min
authors.initials: HM
authors.lastname: Chen
authors.forename: Yi-Chao
authors.initials: YC
authors.lastname: Lee
authors.forename: Chuen-Lin
authors.initials: CL
authors.lastname: Huang
authors.forename: Hui-Kang
authors.initials: HK
authors.lastname: Liu
authors.forename: Wei-Chen
authors.initials: WC
authors.lastname: Liao
authors.forename: Wen-Lin
authors.initials: WL
authors.lastname: Lai
authors.forename: Yen-Ru
authors.initials: YR
authors.lastname: Lin
authors.forename: Nai-Kuei
authors.initials: NK
authors.lastname: Huang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D008694:Methamphetamine / Q000494:pharmacology*; D016716:PC12 Cells; D010544:Peroxidases / Q000378:metabolism*; D054464:Peroxiredoxins; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects*; D013879:Thioredoxins / Q000378:metabolism*
chemical_list: D008694:Methamphetamine; D013879:Thioredoxins; D010544:Peroxidases; D054464:Peroxiredoxins