Manipulation of cell-sized phospholipid-coated microdroplets and their use as biochemical microreactors.
Cell-sized water droplets coated by a phospholipid layer mimicking the inner surface of living cells were manipulated by laser tweezers and used as biochemical microreactors. The cell-sized phospholipid-coated microdroplets (CPMDs) consisted of a water droplet in mineral oil with a diameter of 1-100 microm and coated by 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine. We monitored the time development of biochemical reactions in a single CPMD obtained after the controlled fusion of two CPMDs containing a substrate and an enzyme, respectively. We present results on two enzymatic reactions: calcein production in the presence of esterase and green fluorescence protein expression.
Hase
M
M
Department of Physics, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan.
Yamada
A
A
Hamada
T
T
Baigl
D
D
Yoshikawa
K
K
D001671:Biochemistry / Q000379:methods*; D002627:Chemistry, Physical / Q000379:methods*; D004798:Enzymes / Q000737:chemistry; D005452:Fluoresceins / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry; D008856:Microscopy, Fluorescence / Q000379:methods; D008858:Microscopy, Phase-Contrast; D052898:Optical Tweezers; D010316:Particle Size; D010743:Phospholipids / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000737:chemistry
D004798:Enzymes; D005452:Fluoresceins; D008081:Liposomes; D010743:Phospholipids; D014867:Water; D049452:Green Fluorescent Proteins; C007740:fluorexon