Mass Production of Ginseng (Panax ginseng) Embryoids on Media Containing High Concentrations of Sugar1.
A lot of ginseng embryoids were produced by culturing on high concentrations of sugar media from the embryogenic tissues obtained by moderately high temperature treatment. When the sucrose concentration was 100 g/l, the number of embryoids produced were over 100 pieces per g of inoculum weight. It was about ten times of that produced by culturing on 30 g/l of sucrose. Glucose showed an effect similar to sucrose on the basis of weight percentage. However, mannitol did not show this effect. The embryoids obtained by these processes redifferentiated to normal plantlets on culturing on the medium containing 30 g/l of sucrose. The saponin components of the tissue containing embryoids showed a similar pattern to those of natural GINSENG by HPLC.
Asaka
I
I
Life Science Center, Iwaki Glass Co. Ltd., Gyoda 1-50-1, Funabashi-shi, Chiba 273, Japan.
Ii
I
I
Hirotani
M
M
Asada
Y
Y
Yoshokawa
T
T
Furuya
T
T