title: Catalytic activity of acetylcholinesterase immobilized on mesoporous molecular sieves.
abstract: MCM-41 and FSM-16 were used for enzyme immobilization on account of their good physical and chemical properties. In this work, the catalytic activity of acetylcholinesterase (AChE) immobilized on these materials was investigated, using neostigmina as AChE inhibitor. The results show that AChE was adsorbed on MCM-41 and on FSM-16-TIPB. AChE immobilized on the latter material maintained 70% of its activity and the material did not hydrolyze ACh (as MCM-41) by itself. Therefore, FSM-16-TIPB was the best material, considering also that when neostigmine was applied to AChE immobilized on FSM-16-TIPB, the activity of AChE decreased as occurs in its free from. Hence, this model could be useful in the evaluation of different kinds of AChE inhibitors, allowing the recycling of enzymes and making possible several assays and thereby, lowering cost.
authors.affiliation: Sección de Estudios de Posgrado e Investigación y Departamento de Bioquímica de la Escuela Superior de Medicina del Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón s/n, 11340 México, D.F., Mexico.
authors.forename: M C
authors.initials: MC
authors.lastname: Rosales-Hernández
authors.forename: J E
authors.initials: JE
authors.lastname: Mendieta-Wejebe
authors.forename: J
authors.initials: J
authors.lastname: Correa-Basurto
authors.forename: J I
authors.initials: JI
authors.lastname: Vázquez-Alcántara
authors.forename: E
authors.initials: E
authors.lastname: Terres-Rojas
authors.forename: J
authors.initials: J
authors.lastname: Trujillo-Ferrara
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry / Q000378:metabolism*; D000327:Adsorption; D000818:Animals; D002384:Catalysis; D002417:Cattle; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D008958:Models, Molecular; D016062:Porosity; D012822:Silicon Dioxide / Q000378:metabolism*; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D014961:X-Ray Diffraction
chemical_list: D004800:Enzymes, Immobilized; D012996:Solutions; D012822:Silicon Dioxide; D000110:Acetylcholinesterase