Sharing of antigens between Plasmodium falciparum and Anopheles albimanus.
The presence of common antigens between Plasmodium falciparum and Anopheles albimanus was demonstrated. Different groups of rabbits were immunized with: crude extract from female An. albimanus (EAaF), red blood cells infected with Plasmodium falciparum (EPfs), and the SPf66 synthetic malaria vaccine. The rabbit's polyclonal antibodies were evaluated by ELISA, Multiple Antigen Blot Assay (MABA), and immunoblotting. All extracts were immunogenic in rabbits according to these three techniques, when they were evaluated against the homologous antigens. Ten molecules were identified in female mosquitoes and also in P. falciparum antigens by the autologous sera. The electrophoretic pattern by SDS-PAGE was different for the three antigens evaluated. Cross-reactions between An. albimanus and P. falciparum were found by ELISA, MABA, and immunoblotting. Anti-P. falciparum and anti-SPf66 antibodies recognized ten and five components in the EAaF crude extract, respectively. Likewise, immune sera against female An. albimanus identified four molecules in the P. falciparum extract antigen. As far as we know, this is the first work that demonstrates shared antigens between anophelines and malaria parasites. This finding could be useful for diagnosis, vaccines, and the study of physiology of the immune response to malaria.
Wide
Albina
A
Cátedra de Parasitología, Instituto de Medicina Tropical, Facultad de Medicina, Escuela de Medicina Luiz Razetti, Universidad Central de Venezuela, Los Chaguaramos 1041-A, Caracas, Venezuela. albina_wide@yahoo.com
Capaldo
Jacinta
J
Zerpa
Noraida
N
Pabon
Rosalba
R
Noda
Alfredo
A
Noya
Belkis
B
González
Julio
J
Noya
Oscar
O
D000818:Animals; D000852:Anopheles / Q000276:immunology*; D000913:Antibodies, Protozoan / Q000096:biosynthesis / Q000276:immunology*; D000953:Antigens, Protozoan / Q000276:immunology*; D003429:Cross Reactions / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D005260:Female; D006790:Host-Parasite Interactions / Q000276:immunology; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D017780:Malaria Vaccines / Q000276:immunology*; D010963:Plasmodium falciparum / Q000276:immunology*; D011817:Rabbits
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000939:Epitopes; D017780:Malaria Vaccines