ICP-MS as a novel detection system for quantitative element-tagged immunoassay of hidden peanut allergens in foods.
A novel ICP-MS-based ELISA immunoassay via element-tagged determination was devised for quantitative analysis of hidden allergens in food. The method was able to detect low amounts of peanuts (down to approximately 2 mg peanuts kg(-1) cereal-based matrix) by using a europium-tagged antibody. Selectivity was proved by the lack of detectable cross-reaction with a number of protein-rich raw materials.
Careri
Maria
M
Dipartimento di Chimica Generale ed Inorganica, Chimica Analitica, Chimica Fisica, Università degli Studi di Parma, Viale G.P. Usberti 17/A, 43100 Parma, Italy.
Elviri
Lisa
L
Mangia
Alessandro
A
Mucchino
Claudio
C
D052179:Antigens, Plant / Q000032:analysis*; D010367:Arachis / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D007553:Isotope Labeling / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods*; D008832:Microchemistry / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D052179:Antigens, Plant