Determination of atractylenolide II in rat plasma by reversed-phase high-performance liquid chromatography.
A method for quantitative determination of atractylenolide II in rat plasma using reversed-phase high-performance liquid chromatography (RP-HPLC) coupled with UV spectrometry was established. From a variety of compounds and solvents tested, atractylenolide III was selected as the internal standard (IS) and ethyl acetate was found to be the best solvent for extracting atractylenolide II from plasma samples. RP-HPLC analysis of the extracts was performed on an analytical column (DIKMA ODS, 150 x 4.6 mm; i.d., 5 microm) equipped with a security guard pre-column system. There was good linearity over the range 0.05-5.0 microg/mL (r > 0.99). The recoveries were more than 90.0% in plasma, and the intra- and inter-day coefficients of variation were less than 10.0% in all cases. The limit of detection (LOD) was 0.025 microg/mL and the lower limit of quantification (LLOQ) was 0.05 microg/mL. The RP-HPLC method was applied to quantitate atractylenolide II in rat plasma within 24 h in a pharmacokinetics study where experimental rats received a single dose of atractylenolide II (60 mg/kg).
Ge
Jian
J
Wuhan Botanical Garden, The Chinese Academy of Sciences, Wuhan 430074, People's Republic of China.
Wang
You-Wei
YW
Lu
Xiao-Cong
XC
Sun
Xiao-Hong
XH
Gong
Fu-Jun
FJ
D000284:Administration, Oral; D000818:Animals; D019540:Area Under Curve; D002138:Calibration / Q000592:standards; D002851:Chromatography, High Pressure Liquid / Q000145:classification / Q000379:methods*; D004355:Drug Stability; D007783:Lactones / Q000008:administration & dosage / Q000097:blood* / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000592:standards; D016014:Linear Models; D010949:Plasma / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012717:Sesquiterpenes / Q000008:administration & dosage / Q000097:blood* / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000592:standards; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D007783:Lactones; D012717:Sesquiterpenes; C458582:atractylenolide II; C424802:atractylenolide III