[Preliminary study on differential expressed genes in T lymphocyte of chronic obstructive pulmonary disease patients with Fei-qi deficiency syndrome].
OBJECTIVE
To study T lymphocyte related genes with differential expression in patients with chronic obstructive pulmonary disease (COPD) of Fei-qi deficiency (FQD) syndrome type by gene chips.


METHODS
Lymphocytes in peripheral blood were isolated by Ficoll technique from blood samples collected from COPD patients of FQD syndrome type, Fei-yin deficiency (FYD) syndrome type, and also from healthy subjects for control. They were sorted and purified by flow cytometry, and the different expressed genes were screened from them by gene chip technique.


RESULTS
There were 15 genes with high differential expression between patients of FQD type and those of FYD syndrome type, and between patients of FQD type and healthy subjects.


CONCLUSIONS
Gene chip technique could be used for studying the gene expression profiles of TCM syndrome, and the T-lymphocyte related genes with differential expression in COPD patients with FQD were screened preliminarily.
Li
Ze-Geng
ZG
Department of Respiratory Disease, The First Affiliated Hospital of Anhui College of TCM, Hefei.
Tong
Jia-Bing
JB
Peng
Bo
B
D000328:Adult; D003937:Diagnosis, Differential; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008516:Medicine, Chinese Traditional; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D029424:Pulmonary Disease, Chronic Obstructive / Q000150:complications / Q000235:genetics* / Q000276:immunology; D013577:Syndrome; D013601:T-Lymphocytes / Q000378:metabolism*; D016711:Yang Deficiency / Q000150:complications* / Q000276:immunology