Preparation and characterization of a specific monoclonal antibody against a new gene product: URG11.
As a new gene, the monoclonal antibody against URG11 protein is not currently available. In this study, one monoclonal antibody (MAb) against URG11 was obtained with standard cell fusion technique and enzymelinked immunosorbent assay (ELISA) screening. The peptide of URG11 used in making the MAb in this study was synthesized as described. One of the newly developed MAbs is named MAb 3D2, the isotype of which is IgG2a. Immunohistochemistry and Western blot showed that MAb 3D2 could recognize URG11 protein in both native and denatured forms. MAb 3D2 will be a useful tool for the functional research of URG11 in future studies.
Zou
Xue
X
State Key Laboratory of Cancer Biology & Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.
Li
Xiaohua
X
Liu
Jie
J
Lian
Zhaorui
Z
Fan
Rui
R
Du
Rui
R
Xie
Huahong
H
Song
Jiugang
J
Fan
Daiming
D
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000912:Antibodies, Neoplasm / Q000302:isolation & purification; D000918:Antibody Specificity; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D006515:Hepatitis B virus / Q000472:pathogenicity; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D008113:Liver Neoplasms / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics* / Q000276:immunology*; D009857:Oncogenes; D013274:Stomach Neoplasms / Q000235:genetics / Q000276:immunology; D015534:Trans-Activators / Q000502:physiology; D015854:Up-Regulation; D054334:Viral Regulatory and Accessory Proteins
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D009363:Neoplasm Proteins; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein