title: [Inflammatory mediators and hematological parameters in cord blood in labor complicated by meconium-stained amniotic fluid].
abstract: OBJECTIVE
  Meconium-stained amniotic fluid at term gestation is a predictor for adverse perinatal outcome and is associated with increased peripartum infections, independent of other risk factors. The aim of our study was to evaluate concentrations of inflammatory mediators (such as cytokine IL-6 and intracellular adhesion molecule ICAM-1) and values of hematological parameters of cord blood in presence or absence of meconium in amniotic fluid in term labor.
  
  
  METHODS
  Cord blood samples were obtained from 66 term normal neonates immediately after birth, Soluble ICAM-1 and IL-6 concentrations were measured with ELISA R&D Systems kits. The umbilical blood specimen was analyzed using an automated hematology cell analyzer. Blood films were stained using May-Grünwald-Giemsa method.
  
  
  RESULTS
  There were no difference in concentrations of ICAM-1 and IL-6 in cord blood in groups with or without meconium-stained amniotic fluid. The mean count of umbilical nucleated red blood cells and white blood cells was significantly higher in meconium group. There was no correlation between the cord blood hematological values and ICAM-1 or IL-6. There was also no correlation between IL-6 and ICAM-I and duration of labor. The mode of delivery influenced cord blood IL-6 levels.
  
  
  CONCLUSIONS
  There was no influence of meconium-stained amniotic fluid on cord blood IL-6 and ICAM-1 levels. Changes in hematological parameters in cord blood in meconium passage can suggest either fetus hypoxia or infection. Significant differences of concentrations of fetal IL-6 were associated with the mode of delivery.
authors.affiliation: Klinika Perinatologii Akademii Medycznej w Białymstoku. redzkosl@amb.edu.pl
authors.forename: Sławomir
authors.initials: S
authors.lastname: Redźko
authors.forename: Jerzy
authors.initials: J
authors.lastname: Przepieść
authors.forename: Beata
authors.initials: B
authors.lastname: Zelazowska
authors.forename: Janusz
authors.initials: J
authors.lastname: Zak
authors.forename: Jolanta
authors.initials: J
authors.lastname: Wysocka
authors.forename: Jan
authors.initials: J
authors.lastname: Urban
mesh_terms: D000653:Amniotic Fluid / Q000737:chemistry / Q000276:immunology*; D015331:Cohort Studies; D036861:Delivery, Obstetric; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005312:Fetal Blood / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D007231:Infant, Newborn; D018836:Inflammation Mediators / Q000378:metabolism*; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D015850:Interleukin-6 / Q000032:analysis; D007743:Labor, Obstetric / Q000276:immunology*; D008297:Male; D008470:Meconium / Q000737:chemistry / Q000276:immunology*; D011247:Pregnancy
chemical_list: D018836:Inflammation Mediators; D015850:Interleukin-6; D018799:Intercellular Adhesion Molecule-1