Evaluation of a serological method for the detection of Taenia saginata cysticercosis using serum and meat juice samples.
Two peptides, HP6-2 and Ts45S-10, were used as antigens for the detection of antibodies against Taenia saginata cysticercosis in serum and meat juice samples using enzyme-linked immunosorbent assay (ELISA). Positive control samples were obtained from animals experimentally infected (serum) and from animals naturally infected (meat juice). The two peptides and a pooled preparation of both peptides were evaluated, and their cut-off points with both sample categories were calculated. ELISA results from these different peptides were compared. Sensitivity and specificity of HP6-2 using serum were calculated as being 100 and 98%, respectively, showing to be higher than the values for the other antigens used. The average optical density (OD) value for negative samples was 0.646, whereas it was 1.702 for the positive control samples. This peptide was used to examine serum samples from animals with cysts and random field serum samples. For meat juice samples the pooled peptides showed the highest sensitivity and specificity, as they were 100 and 95%, respectively. The average OD values for the negative and the positive reference meat juice samples were 0.379 and 1.291, respectively. The optimal dilution of the meat juice samples for the ELISA was very low, as it was 1:20 using the pooled peptides, compared with 1:800 serum dilution using HP6-2. To the authors' knowledge, this is the first report of a successful testing for T. saginata cysticercosis using meat juice.
Abuseir
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S
Centre for Food Science, Institute for Food Quality and Safety, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany.
Kühne
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M
Schnieder
T
T
Klein
G
G
Epe
C
C
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000175:diagnosis*; D003551:Cysticercosis / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D008460:Meat / Q000469:parasitology; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000662:veterinary*; D041202:Taenia saginata / Q000302:isolation & purification*