Tracking of human Y receptors in living cells--a fluorescence approach.
Non-invasive methods for studying biological processes in living cells have become very important, also in the field of GPCR biochemistry. Great advancements in the application of fluorescence techniques as well as in the development and improvement of novel fluorophores allow the visualization of dynamic processes. Using these technologies, problems concerning receptor biosynthesis, internalization, recycling and degradation can be investigated. Here we compare the application of the different fluorescent tags EYFP, Lumiotrade mark and SNAPtrade mark to track hY(1) and hY(5) receptors in living cells.
Böhme
Ilka
I
Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, University of Leipzig, Brüderstr. 34, D-04103 Leipzig, Germany.
Mörl
Karin
K
Bamming
Darja
D
Meyer
Cindy
C
Beck-Sickinger
Annette G
AG
D001483:Base Sequence; D017931:DNA Primers; D006801:Humans; D008856:Microscopy, Fluorescence; D017476:Receptors, Neuropeptide Y / Q000378:metabolism*; D015398:Signal Transduction
D017931:DNA Primers; D017476:Receptors, Neuropeptide Y