title: hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes.
abstract: Complete sets of cloned protein-encoding open reading frames (ORFs), or ORFeomes, are essential tools for large-scale proteomics and systems biology studies. Here we describe human ORFeome version 3.1 (hORFeome v3.1), currently the largest publicly available resource of full-length human ORFs (available at ). Generated by Gateway recombinational cloning, this collection contains 12,212 ORFs, representing 10,214 human genes, and corresponds to a 51% expansion of the original hORFeome v1.1. An online human ORFeome database, hORFDB, was built and serves as the central repository for all cloned human ORFs (http://horfdb.dfci.harvard.edu). This expansion of the original ORFeome resource greatly increases the potential experimental search space for large-scale proteomics studies, which will lead to the generation of more comprehensive datasets.
authors.affiliation: Center for Cancer Systems Biology and Department of Cancer Biology, Dana-Farber Cancer Institute, and Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Philippe
authors.initials: P
authors.lastname: Lamesch
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Stuart
authors.initials: S
authors.lastname: Milstein
authors.forename: Changyu
authors.initials: C
authors.lastname: Fan
authors.forename: Tong
authors.initials: T
authors.lastname: Hao
authors.forename: Gabor
authors.initials: G
authors.lastname: Szabo
authors.forename: Zhenjun
authors.initials: Z
authors.lastname: Hu
authors.forename: Kavitha
authors.initials: K
authors.lastname: Venkatesan
authors.forename: Graeme
authors.initials: G
authors.lastname: Bethel
authors.forename: Paul
authors.initials: P
authors.lastname: Martin
authors.forename: Jane
authors.initials: J
authors.lastname: Rogers
authors.forename: Stephanie
authors.initials: S
authors.lastname: Lawlor
authors.forename: Stuart
authors.initials: S
authors.lastname: McLaren
authors.forename: Amélie
authors.initials: A
authors.lastname: Dricot
authors.forename: Heather
authors.initials: H
authors.lastname: Borick
authors.forename: Michael E
authors.initials: ME
authors.lastname: Cusick
authors.forename: Jean
authors.initials: J
authors.lastname: Vandenhaute
authors.forename: Ian
authors.initials: I
authors.lastname: Dunham
authors.forename: David E
authors.initials: DE
authors.lastname: Hill
authors.forename: Marc
authors.initials: M
authors.lastname: Vidal
mesh_terms: D000818:Animals; D002877:Chromosomes, Human; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary; D030561:Databases, Nucleic Acid; D020022:Genetic Predisposition to Disease; D015894:Genome, Human; D006801:Humans; D020407:Internet; D016366:Open Reading Frames; D040901:Proteomics; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary