Detection of chromatin-associated single-stranded DNA in regions targeted for somatic hypermutation.
After encounter with antigen, the antibody repertoire is shaped by somatic hypermutation (SHM), which leads to an increase in the affinity of antibodies for the antigen, and class-switch recombination (CSR), which results in a change in the effector function of antibodies. Both SHM and CSR are initiated by activation-induced cytidine deaminase (AID), which deaminates deoxycytidine to deoxyuridine in single-stranded DNA (ssDNA). The precise mechanism responsible for the formation of ssDNA in V regions undergoing SHM has yet to be experimentally established. In this study, we searched for ssDNA in mutating V regions in which DNA-protein complexes were preserved in the context of chromatin in human B cell lines and in primary mouse B cells. We found that V regions that undergo SHM were enriched in short patches of ssDNA, rather than R loops, on both the coding and noncoding strands. Detection of these patches depended on the presence of DNA-associated proteins and required active transcription. Consistent with this, we found that both DNA strands in the V region were transcribed. We conclude that regions of DNA that are targets of SHM assemble protein-DNA complexes in which ssDNA is exposed, making it accessible to AID.
Ronai
Diana
D
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
Iglesias-Ussel
Maria D
MD
Fan
Manxia
M
Li
Ziqiang
Z
Martin
Alberto
A
Scharff
Matthew D
MD
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D001483:Base Sequence; D002460:Cell Line; D002478:Cells, Cultured; D002843:Chromatin / Q000235:genetics*; D003564:Cytidine Deaminase / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D019169:Jurkat Cells; D051379:Mice; D018345:Mice, Knockout; D027041:Somatic Hypermutation, Immunoglobulin; D014158:Transcription, Genetic; D000098830:AICDA (Activation-Induced Cytidine Deaminase)
D002843:Chromatin; D004277:DNA, Single-Stranded; D000098830:AICDA (Activation-Induced Cytidine Deaminase); D003564:Cytidine Deaminase