Enhanced production of p24 Gag protein in HIV-1-infected rat cells fused with uninfected human cells.
Although many human molecules have been suggested to affect replication of human immunodeficiency virus type 1 (HIV-1), the distribution of such cofactors in human cell types is not well understood. Rat W31/D4R4 fibroblasts expressing human CD4 and CXCR4 receptors were infected with HIV-1. The provirus was integrated in the host genome, but only a limited amount of p24 Gag protein was produced in the cells and culture supernatants. Here we found that p24 production was significantly increased by fusing HIV-1-infected W31/D4R4 cells with uninfected human cell lines of T-cell, B-cell, or macrophage lineages. These findings suggest that human cellular factors supporting HIV-1 replication are distributed widely in cells of lymphocyte and macrophage lineages. We also examined whether the amount of p24 produced by rat-human hybrid cells was correlated with expression levels of specific human genes. The results suggested that HP68 and MHC class II transactivator (CIITA) might up- and down-regulate p24 production, respectively. It was also suggested that HIV-1 replication is affected by molecules other than those examined in this study, namely, cyclin T1, cyclin-dependent kinase 9, CRM1, HP68, and CIITA.
Chen
Jing
J
Department of Pathology, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, Japan.
Zhao
Xudong
X
Lai
Yurong
Y
Suzuki
Akira
A
Tomaru
Utano
U
Ishizu
Akihiro
A
Takada
Akio
A
Ikeda
Hitoshi
H
Kasahara
Masanori
M
Yoshiki
Takashi
T
D000818:Animals; D002459:Cell Fusion; D002460:Cell Line; D005786:Gene Expression Regulation; D016655:HIV Core Protein p24 / Q000096:biosynthesis*; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D006822:Hybrid Cells / Q000378:metabolism*; D051381:Rats
D016655:HIV Core Protein p24