Simple proteomic checks for detecting noncoding RNA.
Proper validation can accelerate sequence-based discovery of proteins and protein-coding genes. Databases currently contain a backlog of experimentally unverified gene models and tentative assignments of observed transcripts to coding or noncoding RNA. We present and apply a general principle, founded on base composition and the genetic code and validated here by bulk 2-D gels, that can improve the reliability of such classifications and of the algorithms or pipelines that lead to them.
Cruveiller
Stéphane
S
Atelier de Génomique Comparative, Genoscope, Centre National de Séquençage, Evry, France.
Clay
Oliver
O
Jabbari
Kamel
K
Bernardi
Giorgio
G
D001482:Base Composition; D015180:Electrophoresis, Gel, Two-Dimensional; D007526:Isoelectric Point; D040901:Proteomics / Q000379:methods; D022661:RNA, Untranslated / Q000032:analysis*
D022661:RNA, Untranslated