title: Analysis of propolis from Baccharis dracunculifolia DC. (Compositae) and its effects on mouse fibroblasts.
abstract: This paper confirms Baccharis dracunculifolia DC. (Compositae) as the main botanical source of the propolis from southeastern Brazil (state of São Paulo) investigated to ascertain specific biological activity in relation to mouse NIH-3T3 fibroblasts, skin cells directly involved in the cicatrization processes. Flavonoid and total phenolic compounds were determined by spectrophotometry, and chemical composition by HPLC; the chromatographic profile, characterized largely by flavonoids and aromatic acids, was found to be qualitatively similar to that of Baccharis dracunculifolia DC. The adsorption of phenolic compounds in the propolis to skin powder was also investigated, and 68% of these compounds adsorbed to the skin powder. At concentrations from 0.12 to 7.81 microg/ml, the propolis revealed no statistical significant differences from its control solutions; however, at concentrations of 31.25 microg/ml or more, the propolis was toxic to NIH-3T3 cells. Thus, the propolis from Baccharis dracunculifolia DC. (Compositae) presents an in vitro concentration-dependent toxicity on mouse NIH-3T3 fibroblasts.
authors.affiliation: Departamento de Farmácia, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Av. Prof. Lineu Prestes, 580, Bloco 15, 05508-900 São Paulo, SP, Brazil. csfunari@gmail.com
authors.forename: Cristiano Soleo
authors.initials: CS
authors.lastname: de Funari
authors.forename: Vicente
authors.initials: V
authors.lastname: de Oliveira Ferro
authors.forename: Monica Beatriz
authors.initials: MB
authors.lastname: Mathor
mesh_terms: D000818:Animals; D031175:Baccharis / Q000033:anatomy & histology / Q000737:chemistry* / Q000145:classification; D001516:Bees; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects*; D005419:Flavonoids / Q000032:analysis; D013058:Mass Spectrometry / Q000379:methods; D051379:Mice; D041681:NIH 3T3 Cells; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry / Q000633:toxicity; D018515:Plant Leaves / Q000737:chemistry; D011429:Propolis / Q000032:analysis* / Q000633:toxicity*; D012996:Solutions / Q000633:toxicity
chemical_list: D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D012996:Solutions; D011429:Propolis