title: [Studies on the interaction between thionine and deoxyribo-nucleic acid by ethidium bromide probe].
abstract: The interaction between thionine and deoxyribonucleic acid was studied with ethidium bromide probe in Tris-HCl buffer solution at pH 7.4 by fluorescence spectra, electronic absorption spectra, and resonance light scattering spectra. From the obtained results, it was found obviously that the planar structure of thionine can be intercalated into the stacked base pairs of DNA, which is the major controlling factor. The binding constant was found to be 1.0 x 10(5) L x mol(-1).
authors.affiliation: College of Chemistry and Environmental Science, Henan Normal University, Xinxiang 453007, China.
authors.forename: Xue-ping
authors.initials: XP
authors.lastname: Liu
authors.forename: Su-ling
authors.initials: SL
authors.lastname: Feng
authors.forename: Zi-hong
authors.initials: ZH
authors.lastname: Pan
authors.forename: Jing
authors.initials: J
authors.lastname: Fan
mesh_terms: D004247:DNA / Q000737:chemistry*; D004996:Ethidium / Q000737:chemistry*; D010640:Phenothiazines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D010640:Phenothiazines; D004247:DNA; D004996:Ethidium; C009469:thionine