High hydrostatic pressure approach proves RNA catalytic activity without magnesium.
High hydrostatic pressure (HHP) technique was used to evaluate a mechanism of RNA hydrolysis with RNA. We showed that hammerhead ribozyme specifically cleaves RNA substrate at HHP in the absence of Mg(2+). A deoxyribozyme "10-23" was active in the same conditions. These results pointed out that the hydrolytic activity of nucleic acid depends on proper tertiary structure of a complex with a substrate. They prove that magnesium ion is not directly involved in catalysis process. On that basis we show the mechanism of RNA hydrolysis catalyzed with nucleic acids at HHP.
Fedoruk-Wyszomirska
Agnieszka
A
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.
Wyszko
Eliza
E
Giel-Pietraszuk
Małgorzata
M
Barciszewska
Mirosława Z
MZ
Barciszewski
Jan
J
D001483:Base Sequence; D006868:Hydrolysis; D006874:Hydrostatic Pressure; D066298:In Vitro Techniques; D008274:Magnesium / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016337:RNA, Catalytic / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D016337:RNA, Catalytic; D012367:RNA, Viral; D008274:Magnesium