Miniature near-infrared dual-axes confocal microscope utilizing a two-dimensional microelectromechanical systems scanner.
The first, to our knowledge, miniature dual-axes confocal microscope has been developed, with an outer diameter of 10 mm, for subsurface imaging of biological tissues with 5-7 microm resolution. Depth-resolved en face images are obtained at 30 frames per second, with a field of view of 800 x 100 microm, by employing a two-dimensional scanning microelectromechanical systems mirror. Reflectance and fluorescence images are obtained with a laser source at 785 nm, demonstrating the ability to perform real-time optical biopsy.
Liu
Jonathan T C
JT
Edward L. Ginzton Laboratory, Stanford University, Stanford, California 94305, USA. jonliu@stanfordalumni.org
Mandella
Michael J
MJ
Ra
Hyejun
H
Wong
Larry K
LK
Solgaard
Olav
O
Kino
Gordon S
GS
Piyawattanametha
Wibool
W
Contag
Christopher H
CH
Wang
Thomas D
TD
D003199:Computer Systems; D004581:Electronics; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D007089:Image Enhancement / Q000295:instrumentation*; D019563:Mechanics; D008846:Micromanipulation / Q000295:instrumentation* / Q000379:methods; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods; D008904:Miniaturization; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013055:Spectrophotometry, Infrared / Q000295:instrumentation* / Q000379:methods; D014159:Transducers