title: Transcription of rat TRPV1 utilizes a dual promoter system that is positively regulated by nerve growth factor.
abstract: The capsaicin receptor, also known as 'transient receptor potential vanilloid receptor subtype 1' (TRPV1, VR1), is an ion channel subunit expressed in primary afferent nociceptors, which plays a critical role in pain transduction and thermal hyperalgesia. Increases in nociceptor TRPV1 mRNA and protein are associated with tissue injury-inflammation. As little is understood about what controls TRPV1 RNA transcription in nociceptors, we functionally characterized the upstream portion of the rat TRPV1 gene. Two functional rTRPV1 promoter regions and their transcription initiation sites were identified. Although both promoter regions directed transcriptional activity in nerve growth factor (NGF) treated rat sensory neurons, the upstream Core promoter was the most active in cultures enriched in sensory neurons. Because NGF is a key modulator of inflammatory pain, we examined the effect of NGF on rTRPV1 transcription in PC12 cells. NGF positively regulated transcriptional activity of both rTRPV1 promoter regions in PC12 cells. We propose that the upstream regulatory region of the rTRPV1 gene is composed of a dual promoter system that is regulated by NGF. These findings support the hypothesis that NGF produced under conditions of tissue injury and/or inflammation directs an increase of TRPV1 expression in nociceptors in part through a transcription-dependent mechanism.
authors.affiliation: Department of Anesthesia and Perioperative Care, University of California San Francisco, San Francisco, California 94143-0427, USA.
authors.forename: Qing
authors.initials: Q
authors.lastname: Xue
authors.forename: Beverly
authors.initials: B
authors.lastname: Jong
authors.forename: Tom
authors.initials: T
authors.lastname: Chen
authors.forename: Mark A
authors.initials: MA
authors.lastname: Schumacher
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence / Q000235:genetics; D002478:Cells, Cultured; D005727:Ganglia, Spinal / Q000187:drug effects / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics; D008969:Molecular Sequence Data; D020932:Nerve Growth Factor / Q000494:pharmacology / Q000502:physiology*; D009475:Neurons, Afferent / Q000187:drug effects / Q000378:metabolism*; D009619:Nociceptors / Q000187:drug effects / Q000378:metabolism*; D016716:PC12 Cells; D010146:Pain / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D011401:Promoter Regions, Genetic / Q000187:drug effects / Q000235:genetics*; D051381:Rats; D050916:TRPV Cation Channels / Q000096:biosynthesis / Q000235:genetics*; D015533:Transcriptional Activation / Q000187:drug effects / Q000235:genetics
chemical_list: D050916:TRPV Cation Channels; C493922:Trpv1 protein, rat; D020932:Nerve Growth Factor