X-ray radiation-induced damage in DNA monitored by online Raman.
High-quality Raman spectra have been collected on single DNA crystals kept at 100 K in a flow of cold nitrogen gas prior, during and after macromolecular crystallography (MX) data collection. The non-symmetric bending vibration for C-Br bonds in 8-bromo-2'-deoxyguanosine has been unambiguously identified at 293 cm(-1) in the non-resonant Raman spectra. The breakage of this bond could be monitored by the continuous recording of Raman spectra during X-ray exposure, and its decay versus dose could be directly correlated with the loss of the bromide definition within the electron-density maps as determined with MX.
McGeehan
John E
JE
EMBL, 6 rue Jules Horowitz, BP 181, 38042 Grenoble CEDEX 9, France. mcgeehan@embl.fr
Carpentier
Philippe
P
Royant
Antoine
A
Bourgeois
Dominique
D
Ravelli
Raimond B G
RB
D003198:Computer Simulation; D003461:Crystallography / Q000379:methods*; D004247:DNA / Q000737:chemistry* / Q000528:radiation effects* / Q000648:ultrastructure; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D008956:Models, Chemical; D008958:Models, Molecular; D009691:Nucleic Acid Denaturation / Q000528:radiation effects; D009862:Online Systems; D011829:Radiation Dosage; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014965:X-Rays
D004247:DNA