[Effect of shenkangwan on TGF-beta1 and FN in rat mesangial cells cultured by high glucose].
OBJECTIVE
To investigate the effect of Shenkangwan on secretion of transforming growth factor-beta1 (TGF-beta1) and Fibronectin (FN) and their mRNA expression in rat mesangial cells (MC)cultured by high glucose and explore its molecule mechanism on treating diabetic nephropathy (DN).


METHODS
Rat MC were cultured in vivo and divided into six groups: low glucose group, high glucose group, normal rat serum group, Capoten tablets group, Shenkangwan high dose group and low dose group. Seventy two hours later, the secretion of TGF-beta1 and FN in MC were detected by enzyme linked immunosorbent assay (ELISA). The expression of TGF-beta1, and FN mRNA were determined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR).


RESULTS
High gluscose could induce secretion of TGF-beta1 and FN in MC and increase expression of TGF-beta1 and FN mRNA. While Shenkangwan could repress these effects.


CONCLUSIONS
Shenkangwan can suppress the secretion of the extracellular matrix in MC via TGF-beta1 signal way.
Xiao
Wei
W
Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Ma
Yun
Y
Wei
Lian-bo
LB
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D003928:Diabetic Nephropathies / Q000473:pathology* / Q000517:prevention & control; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D005353:Fibronectins / Q000096:biosynthesis* / Q000235:genetics; D005947:Glucose / Q000494:pharmacology; D008297:Male; D050527:Mesangial Cells / Q000187:drug effects* / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics
D003470:Culture Media; D004365:Drugs, Chinese Herbal; D005353:Fibronectins; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D005947:Glucose