title: Biochemical composition of ovine follicular fluid in relation to follicle size.
abstract: The aim of this study was to biochemically characterize ovine follicular fluid and to relate possible changes in composition to follicular size. Ovaries were collected from adult and cycling non-pregnant slaughtered sheep (Ovis aries) during breeding season. A total of 104 pairs of ovaries were investigated and these data were then compared. Follicular fluid was aspirated from small (< 2 mm), medium (2-4 mm) and large (> 4 mm) nonatretic ovarian follicles. The follicular fluid was centrifuged at 4 degrees C and 5000 g for 30 min to remove any cells and stored at -80 degrees C prior to assay. Follicular fluid samples were analyzed for glucose, total protein, cholesterol, triglycerides, lactate, urea, creatinine, sodium, potassium, chloride, calcium, phosphorus, magnesium, acid phosphatase, alkaline phosphatase, and lactate dehydrogenase. Data were analyzed by the linear regression model. As follicles became larger, the concentrations of glucose and cholesterol significantly (P < 0.05) increased while those of triglycerides, lactate, alkaline phosphatase and lactate dehydrogenase significantly (P < 0.05) decreased.
authors.affiliation: National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore 560030, India. snandi71@yahoo.co.in
authors.forename: S
authors.initials: S
authors.lastname: Nandi
authors.forename: V Girish
authors.initials: VG
authors.lastname: Kumar
authors.forename: B M
authors.initials: BM
authors.lastname: Manjunatha
authors.forename: P S P
authors.initials: PS
authors.lastname: Gupta
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D002784:Cholesterol / Q000032:analysis; D005260:Female; D015571:Follicular Fluid / Q000378:metabolism*; D005947:Glucose / Q000032:analysis; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D009929:Organ Size / Q000502:physiology; D034561:Sheep, Domestic; D014280:Triglycerides / Q000032:analysis
chemical_list: D014280:Triglycerides; D002784:Cholesterol; D007770:L-Lactate Dehydrogenase; D000469:Alkaline Phosphatase; D005947:Glucose