title: [Silencing of c-myc gene expression by enzymatically and chemically synthesized siRNAs].
abstract: Small interfering RNAs (siRNA) provide a powerful approach for sequence-specific silencing of gene expression. In the present study we investigated inhibition of c-myc gene expression by siRNAs targeted to the sequence 1452-1470 b. in third exon of c-myc mRNA and to homologous regions in second exons of c-myc (697-715 b.) and N-myc (302-320 b.) mRNAs. siRNAs were prepared enzymatically according to the scheme, including dsDNA-templates preparation using Klenow fragment, separate in vitro transcription of each RNA strand with subsequent hybridization and removal of leader sequences by T1 RNase. Investigation of c-myc gene silencing by siRNAs revealed that enzymatically prepared siRNAs induce stronger inhibition of c-myc expression, than siRNA with the same sequence prepared by chemical synthesis. It was found that down-regulation of c-myc gene expression by investigated siRNAs results in efficient inhibition and even complete arrest of carcinoma cell proliferation, moreover, the extend of growth inhibition correlates with the level of siRNA-mediated reduction of c-myc mRNA.
authors.forename: T O
authors.initials: TO
authors.lastname: Kabilova
authors.forename: A V
authors.initials: AV
authors.lastname: Vladimirova
authors.forename: M N
authors.initials: MN
authors.lastname: Repkova
authors.forename: A G
authors.initials: AG
authors.lastname: Ven'iaminova
authors.forename: E L
authors.initials: EL
authors.lastname: Chernolovskaia
authors.forename: V V
authors.initials: VV
authors.lastname: Vlasov
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects*; D016259:Genes, myc / Q000187:drug effects; D006801:Humans; D008969:Molecular Sequence Data; D016271:Proto-Oncogene Proteins c-myc / Q000037:antagonists & inhibitors* / Q000235:genetics*; D034741:RNA, Small Interfering / Q000096:biosynthesis* / Q000138:chemical synthesis / Q000494:pharmacology*
chemical_list: D016271:Proto-Oncogene Proteins c-myc; D034741:RNA, Small Interfering