Cryopreservation of sheep primordial follicles.
The aim of this study was to evaluate the efficiency of 1 M dimethylsulphoxide (DMSO), ethylene glycol (EG), propylene glycol (PROH) and glycerol (GLY) to cryopreserve primordial follicles. The first evaluation was performed soon after cryopreservation and the second evaluation after 4 days of in vitro culture, using the cryoprotectants that allowed the higher results (higher follicular survival rate) after cryopreservation. The results after follicular isolation (control) and cryopreservation using 1 M DMSO, EG, PROH and GLY showed that the mean number (+/- SEM) of live follicles per millilitre was 3204 (100%) +/- 319.27, 2798 (87%) +/- 239.14, 2492 (78%) +/- 345.8, 448 (14%) +/- 46.3 and 208 (7%) +/- 75.26, respectively. Higher follicular survival was reported when DMSO and EG were used. Control follicles and follicles cryopreserved with these two cryoprotectants were cultured and the percentage of follicular survival was 55% (control), 42% (EG) and 34% (DMSO). Similar results were found between control and follicles cryopreserved with EG. In conclusion, 1 M EG is the most effective cryoprotectant to preserve primordial follicles isolated from ovaries of sheep.
Amorim
C A
CA
Programa de Pós-graduação em Medicina Veterinária, Centro de Ciências Rurais, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil. amorimchris@yahoo.com
Rondina
D
D
Lucci
C M
CM
Giorgetti
A
A
de Figueiredo
J R
JR
Gonçalves
P B D
PB
D000818:Animals; D002470:Cell Survival; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D005260:Female; D005990:Glycerol / Q000494:pharmacology; D006080:Ovarian Follicle / Q000502:physiology*; D019946:Propylene Glycol / Q000494:pharmacology; D012756:Sheep; D018675:Toxicity Tests
D003451:Cryoprotective Agents; D019946:Propylene Glycol; D005990:Glycerol