title: Humicoccus flavidus gen. nov., sp. nov., isolated from soil.
abstract: A Gram-positive, non-motile, spherical, non-spore-forming bacterial strain, DS-52(T), was isolated from soil from Dokdo, Korea, and its taxonomic position was investigated by using a polyphasic approach. It grew optimally at 25 degrees C and pH 6.0-7.0. Strain DS-52(T) had meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan, and galactose, mannose, xylose and rhamnose as whole-cell sugars. It contained MK-8(H(4)) and MK-9(H(4)) as the predominant menaquinones and anteiso-C(15 : 0), iso-C(15 : 0) and C(17 : 0) as major fatty acids. Major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidyldimethylethanolamine. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain DS-52(T) is most closely related to the genus Nakamurella of the suborder Frankineae. Strain DS-52(T) exhibited 16S rRNA gene sequence similarity values of 96.5 % to Nakamurella multipartita JCM 9543(T) and 92.0-93.9 % to other members of the suborder Frankineae. The diagnostic diamino acid type and polar lipid profile of strain DS-52(T) were the same as those of the genus Nakamurella. However, strain DS-52(T) could be clearly distinguished from the genus Nakamurella by differences in predominant menaquinones, major fatty acids and cell-wall sugars. Accordingly, based on combined phenotypic, chemotaxonomic and phylogenetic data, strain DS-52(T) (=KCTC 19127(T)=CIP 108919(T)) is proposed as the type strain of a novel species in a new genus, Humicoccus flavidus gen. nov., sp. nov.
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
authors.forename: So-Jung
authors.initials: SJ
authors.lastname: Kang
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
authors.forename: Seo-Youn
authors.initials: SY
authors.lastname: Jung
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
authors.forename: Tae-Kwang
authors.initials: TK
authors.lastname: Oh
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D006095:Gram-Positive Cocci / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S