Comparative analysis of a monoclonal antibody-based Streptococcus mutans detection method with selective culture assays using polymerase chain reaction as a gold standard.
The aim of this study was to compare a recently developed monoclonal antibody (MAb)-based salivary Streptococcus mutans detection method with various selective media using polymerase chain reaction (PCR) as the gold standard. Salivary S. mutans cells were enumerated with a MAb-based method, along with three commonly used selective media, mitis-salivarius-bacitracin agar (MSB), trypticase yeast-extract cystine sucrose bacitracin agar (TYCSB), and glucose-sucrose-potassium tellurite-bacitracin (GSTB) agar. Statistical analysis showed no significant correlations between each method. With PCR as the standard, a MAb-based detection method was found to provide the highest sensitivity (91%) and specificity (96%) among these four methods. This study demonstrates that the MAb-based detection method may provide more accurate enumeration of salivary S. mutans than selective media.
Gu
Fang
F
School of Dentistry and Dental Research Institute, University of California, Los Angeles, California 900-1668, USA.
Qi
Fengxia
F
Anderson
Maxwell H
MH
Shi
Wenyuan
W
D000818:Animals; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001431:Bacteriological Techniques / Q000706:statistics & numerical data; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D051379:Mice; D016133:Polymerase Chain Reaction / Q000706:statistics & numerical data; D012463:Saliva / Q000382:microbiology; D012680:Sensitivity and Specificity; D013295:Streptococcus mutans / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D004269:DNA, Bacterial