title: Generic detection of coronaviruses and differentiation at the prototype strain level by reverse transcription-PCR and nonfluorescent low-density microarray.
abstract: A nonfluorescent low-cost, low-density oligonucleotide array was designed for detecting the whole coronavirus genus after reverse transcription (RT)-PCR. The limit of detection was 15.7 copies/reaction. The clinical detection limit in patients with severe acute respiratory syndrome was 100 copies/sample. In 39 children suffering from coronavirus 229E, NL63, OC43, or HKU1, the sensitivity was equal to that of individual real-time RT-PCRs.
authors.affiliation: Clinical Virology Section, Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Str. 74, 20359 Hamburg, Germany.
authors.forename: Luciano Kleber
authors.initials: LK
authors.lastname: de Souza Luna
authors.forename: Volker
authors.initials: V
authors.lastname: Heiser
authors.forename: Nicolas
authors.initials: N
authors.lastname: Regamey
authors.forename: Marcus
authors.initials: M
authors.lastname: Panning
authors.forename: Jan Felix
authors.initials: JF
authors.lastname: Drexler
authors.forename: Sabue
authors.initials: S
authors.lastname: Mulangu
authors.forename: Leo
authors.initials: L
authors.lastname: Poon
authors.forename: Sigrid
authors.initials: S
authors.lastname: Baumgarte
authors.forename: Bert Jan
authors.initials: BJ
authors.lastname: Haijema
authors.forename: Laurent
authors.initials: L
authors.lastname: Kaiser
authors.forename: Christian
authors.initials: C
authors.lastname: Drosten
mesh_terms: D015373:Bacterial Typing Techniques; D017934:Coronavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D028941:Coronavirus 229E, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D028962:Coronavirus OC43, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D045473:Severe acute respiratory syndrome-related coronavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D013045:Species Specificity
chemical_list: D012367:RNA, Viral