Production, formulation and antagonistic activity of the biocontrol like-yeast Aureobasidium pullulans against Penicillium expansum.
Aureobasidium pullulans (de Bary) Arnaud (Ach 1-1) was grown in a glucose fed-batch fermentor to 106 g dry wt l(-1) in 48 h. The cells were dried in a fluidized bed dryer with a final viability of 62%. After 7 months at 4 degrees C, the viability was 28% of the initial value (= 2.3 x 10(10 )c.f.u. g(-1) dry matter). A protection level of 89% was achieved with the biomass preparation at 1 x 10(8 )c.f.u. ml(-1) after 28 and 7 days for apples stored respectively at 5 and 25 degrees C against Penicillium expansum. Our process is suitable to produce large quantities of the strain Ach 1-1 as biological control agent for apple preservation.
Mounir
R
R
Laboratoire de Phytobactériologie, Institut National de la Recherche Agronomique, Meknès VN, Morocco.
Durieux
A
A
Bodo
E
E
Allard
C
C
Simon
J-P
JP
Achbani
E-H
EH
El-Jaafari
S
S
Douira
A
A
Jijakli
M-H
MH
D001203:Ascomycota / Q000166:cytology* / Q000502:physiology*; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002470:Cell Survival; D018920:Coculture Techniques / Q000379:methods; D005659:Fungicides, Industrial; D010407:Penicillium / Q000166:cytology* / Q000502:physiology*; D010572:Pest Control, Biological / Q000379:methods
D005659:Fungicides, Industrial