Oxidative stress induced lipocalin 2 gene expression: addressing its expression under the harmful conditions.
Lipocalin 2 (Lcn2, NGAL) is a member of the lipocalin superfamily with diverse functions such as the transport of fatty acids and the induction of apoptosis. Previous reports indicated that expression of Lcn2 is induced under harmful conditions. However, the mechanisms of the induction of Lcn2 expression remain to be elucidated. In this report, we intended to identify the factor or factors that induce Lcn2 expression. Up-regulation of Lcn2 expression after X-ray exposure was detected in the heart, the kidney and especially in the liver. Primary culture of liver component cells revealed that this up-regulation in the liver was induced in hepatocytes. Up-regulation of Lcn2 expression was also detected in HepG2 cells after the administration of X-rays or H(2)O(2). Interestingly, up-regulation of Lcn2 expression after H(2)O(2) treatment was canceled by the addition of the anti-oxidants, dimethylsulfoxide or cysteamine. These results strongly suggest that Lcn2 expression is induced by reactive oxygen species. Therefore, Lcn2 could be a useful biomarker to identify oxidative stress both in vitro and in vivo.
Roudkenar
Mehryar Habibi
MH
Department of Pathology, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan.
Kuwahara
Yoshikazu
Y
Baba
Taisuke
T
Roushandeh
Amaneh Mohammadi
AM
Ebishima
Shigeko
S
Abe
Shinya
S
Ohkubo
Yasuhito
Y
Fukumoto
Manabu
M
D000209:Acute-Phase Proteins / Q000378:metabolism*; D000818:Animals; D004307:Dose-Response Relationship, Radiation; D015870:Gene Expression / Q000502:physiology* / Q000528:radiation effects*; D022781:Hepatocytes / Q000378:metabolism* / Q000528:radiation effects*; D000071068:Lipocalin-2; D054834:Lipocalins; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D015513:Oncogene Proteins / Q000378:metabolism*; D009928:Organ Specificity; D018384:Oxidative Stress / Q000502:physiology* / Q000528:radiation effects*; D011829:Radiation Dosage; D014018:Tissue Distribution
D000209:Acute-Phase Proteins; D000071068:Lipocalin-2; D054834:Lipocalins; D015513:Oncogene Proteins; C071332:Lcn2 protein, mouse