Production of herbicide-resistant sweet potato plants transformed with the bar gene.
Herbicide-resistant sweet potato plants were produced through biolistics of embryogenic calli derived from shoot apical meristems. Plant materials were bombarded with the vectors containing the beta-glucuronidase gene (gusA) and the herbicide-resistant gene (bar). Selection was carried out using phosphinothricin (PPT). Transformants were screened by the histochemical GUS and Chlorophenol Red assays. PCR and Southern-blot analyses indicated the presence of introduced bar gene in the genomic DNA of the transgenic plants. When sprayed with Basta, the transgenic sweet potato plants was tolerant to the herbicide. Hence, we report successful transformation of the bar gene conferring herbicide resistance to sweet potato.
Yi
Gibum
G
Kumho Life and Environmental Science Laboratory, Chonnam National University, Gwangju, Korea.
Shin
Young-Mi
YM
Choe
Goh
G
Shin
Byoungchul
B
Kim
Young Soon
YS
Kim
Kyung-Moon
KM
D053209:Herbicide Resistance / Q000502:physiology*; D006540:Herbicides / Q000008:administration & dosage*; D027723:Ipomoea batatas / Q000187:drug effects / Q000502:physiology*; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000502:physiology*
D006540:Herbicides; D010940:Plant Proteins