Topical mitomycin C effect on swine vocal folds healing.
Several adjuvant therapies to surgery have been used to modulate the healing process of vocal folds, including topic mitomycin (MTC).


OBJECTIVE
to evaluate the effect of topical MTC in the healing process of vocal folds 30 days after the exeresis of a mucosal fragment with CO2 laser in a swine model (control group without mitomycin and study group with topical MTC), with collagen deposition measurement.


METHODS
prospective experimental in swine.


METHODS
two groups of 6 swine each were subjected to exeresis of a mucosal fragment of the vocal fold with CO2 laser. Immediately after the procedure MTC was applied topically for three minutes on the study group. Thirty days later the animals were slaughtered and samples of the vocal folds were collected for histological analysis, with the purpose of quantifying collagen deposition by Picrosirius Red stain.


RESULTS
the average area of total collagen in the vocal folds in the control group was 2.648,03 microm2, whereas in the study group it was 2.200,30 microm2 (p=0.0043).


CONCLUSIONS
topical application of MTC after the exeresis of a mucous fragment of vocal fold with CO2 laser in swine significantly decreased total collagen deposition.
Camargo
Paulo Antônio Monteiro
PA
Serviço de Otologia e Laringologia, Otorrinolaringologia Department, Hospital Angelina Caron, Curitiba, Pr, Brazil. cao.pr@terra.com.br
Campos
Antonio Carlos Ligocki
AC
Matias
Jorge Eduardo Fouto
JE
Rispoli
Daniel Zeni
DZ
Przysiezny
Paulo Eduardo
PE
Fonseca
Vinícius Ribas
VR
D000287:Administration, Topical; D000477:Alkylating Agents / Q000008:administration & dosage*; D000818:Animals; D003094:Collagen / Q000032:analysis* / Q000187:drug effects; D005260:Female; D053685:Laser Therapy; D008297:Male; D016685:Mitomycin / Q000008:administration & dosage*; D013552:Swine; D013997:Time Factors; D014827:Vocal Cords / Q000473:pathology / Q000601:surgery*; D014945:Wound Healing / Q000187:drug effects*
D000477:Alkylating Agents; D016685:Mitomycin; D003094:Collagen