Human bronchial epithelial cell transcriptome: gene expression changes following acute exposure to whole cigarette smoke in vitro.
Cigarette smoke is a complex mixture of more than 4,000 constituents. Its effects on cell biology are poorly understood, partly because whole smoke exposure in vitro is technically challenging. To investigate the effects of smoke on cell signaling and function, a three-dimensional air-liquid interface model of tracheobronchial epithelium, grown from primary human lung epithelial cells, was exposed to air or whole mainstream cigarette smoke for 1 h in a purpose-designed chamber. Gene expression profiles were then determined at 1, 6, and 24 h postexposure using Affymetrix HGU133-2 Plus microarrays. Cells from three different donors were used in the study, and the experiment was performed in triplicate for each donor. Genes significantly regulated by smoke, compared with the air control, in all experiments were determined. Genes exhibiting differential expression were assigned to functional categories and mapped to signaling pathways. Effects were observed on many cellular processes including xenobiotic metabolism, oxidant/antioxidant balance, and DNA damage and repair. Notably, there was marked downregulation of the transforming growth factor-beta pathway, which has not been previously reported. This study provides important data on the acute effects of whole cigarette smoke on mucociliary epithelium and may be used to gain a greater understanding of smoke toxicity.
Maunders
Heather
H
Advanced Technologies Cambridge, Cambridge, United Kingdom.
Patwardhan
Sudhanshu
S
Phillips
Jeremy
J
Clack
Aaron
A
Richter
Audrey
A
D001980:Bronchi / Q000166:cytology / Q000473:pathology / Q000502:physiology*; D005786:Gene Expression Regulation; D006801:Humans; D007700:Kinetics; D020935:MAP Kinase Signaling System / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D020545:Respiratory Mucosa / Q000166:cytology / Q000473:pathology / Q000502:physiology*; D012906:Smoke / Q000009:adverse effects*; D014158:Transcription, Genetic
D012906:Smoke; D012313:RNA