title: Use of an insect cell culture growth medium to isolate bacteria from horses with effusive, fibrinous pericarditis: a preliminary study.
abstract: Effusive, fibrinous pericarditis is an uncommon disease entity in horses. In 2001, pericarditis occurred in conjunction with an epizootic in central Kentucky that was associated with exposure to eastern tent caterpillars (ETCs). Bacterial isolation from equine pericardial fluid samples was attempted using an insect cell culture growth medium (ICCGM). Using previously cultured, stored frozen samples from four horses with fibrinous pericarditis, inoculation of 10% blood agar plates yielded no growth, whereas simultaneous inoculation of ICCGM resulted in the isolation of Proprionibacterium acnes, Staphylococcus equorum, a Streptococcus sp. and Pseudomonas rhodesiae from pericardial fluid samples. A similar or novel caterpillar-associated bacteria was not identified; however, use of an ICCGM might enhance isolation of bacteria from equine pericardial fluid.
authors.affiliation: Intracellular Pathogens Research Laboratory, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA.
authors.forename: Samuel L
authors.initials: SL
authors.lastname: Jones
authors.forename: Amy
authors.initials: A
authors.lastname: Valenzisi
authors.forename: Sushama
authors.initials: S
authors.lastname: Sontakke
authors.forename: Kimberly A
authors.initials: KA
authors.lastname: Sprayberry
authors.forename: Ricardo
authors.initials: R
authors.lastname: Maggi
authors.forename: Barbara
authors.initials: B
authors.lastname: Hegarty
authors.forename: Edward
authors.initials: E
authors.lastname: Breitschwerdt
mesh_terms: D000818:Animals; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D003470:Culture Media; D046508:Culture Techniques / Q000662:veterinary; D004196:Disease Outbreaks / Q000662:veterinary*; D006734:Horse Diseases / Q000382:microbiology* / Q000473:pathology; D006736:Horses; D007313:Insecta / Q000166:cytology / Q000382:microbiology; D007629:Kentucky / Q000453:epidemiology; D010490:Pericardial Effusion / Q000382:microbiology / Q000473:pathology; D010493:Pericarditis / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D010865:Pilot Projects; D012189:Retrospective Studies
chemical_list: D003470:Culture Media