title: Incorporation of [1-14C]-Isopentenyl Pyrophosphate into Monoterpenes by a Cell-Free Homogenate Prepared from Callus Cultures of Chrysanthemum cinerariaefolium.
abstract: Callus culture of CHRYSANTHEMUM CINERARIAEFOLIUM (Trev.) Bocc. (Asteraceae), initiated with axillary buds from the plant, have been shown to produce chrysanthemyl alcohol and chrysanthemic acid. Incubation of a cell-free homogenate from the callus with [1- (14)C]-isopentenyl pyrophosphate demonstrated incorporation of radioactivity into chrysanthemyl alcohol and chrysanthemic acid as well as products from isopentenyl pyrophosphate isomerase (EC 5.3.3.2; isopentenyl diphosphate Delta (3)-Delta (2)-isomerase) and prenyltransferase activities (EC 2.5.1.11; allylic-terpene-diphosphate:isopentenyl diphosphate terpenoid-allyltransferase).
authors.affiliation: Department of Pharmaceutical Sciences, St. John's University, College of Pharmacy and Allied Health Professions, Jamaica, New York 11439, U.S.A.
authors.forename: S W
authors.initials: SW
authors.lastname: Zito
authors.forename: V
authors.initials: V
authors.lastname: Srivastava
authors.forename: E
authors.initials: E
authors.lastname: Adebayo-Olojo