title: DNA-methyltransferase SsoII as a bifunctional protein: features of the interaction with the promoter region of SsoII restriction-modification genes.
abstract: DNA duplexes bearing an aldehyde group at the 2'-position of the sugar moiety were used for affinity modification of (cytosine-5)-DNA methyltransferase SsoII. It is shown that lysine residues of M.SsoII N-terminal region are located in proximity to DNA sugar-phosphate backbone of a regulatory sequence of promoter region of SsoII restriction-modification enzyme coding genes. The ability of the two M.SsoII subunits to interact with DNA regulatory sequence has been demonstrated by affinity modification using DNA duplexes with two 2'-aldehyde groups. Changes in nucleotide sequence of one half of the regulatory region prevented cross-linking of the second M.SsoII subunit. The results on sequential affinity modification of M.SsoII by two types of modified DNA ligands (i.e. by 2'-aldehyde-containing and phosphoryldisulfide-containing) have demonstrated the possibility of covalent attachment of the protein to two different DNA recognition sites: regulatory sequence and methylation site.
authors.affiliation: Chemical Faculty, Lomonosov Moscow State University, Moscow, 119992, Russia.
authors.forename: A S
authors.initials: AS
authors.lastname: Romanenkov
authors.forename: O V
authors.initials: OV
authors.lastname: Kisil
authors.forename: T S
authors.initials: TS
authors.lastname: Zatsepin
authors.forename: O V
authors.initials: OV
authors.lastname: Iamskova
authors.forename: A S
authors.initials: AS
authors.lastname: Kariagina
authors.forename: V G
authors.initials: VG
authors.lastname: Metelev
authors.forename: T S
authors.initials: TS
authors.lastname: Oretskaia
authors.forename: E A
authors.initials: EA
authors.lastname: Kubareva
mesh_terms: D020134:Catalytic Domain; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D015280:DNA Restriction-Modification Enzymes / Q000737:chemistry* / Q000378:metabolism; D015257:DNA-Cytosine Methylases / Q000737:chemistry* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding
chemical_list: D015280:DNA Restriction-Modification Enzymes; D004247:DNA; C063974:DNA modification methylase SsoII; D015257:DNA-Cytosine Methylases