title: Transient transmission of porcine endogenous retrovirus to fetal lambs after pig islet tissue xenotransplantation.
abstract: Evidence for the in vivo transmission of porcine endogenous retrovirus (PERV) from porcine xenografts to various recipient animals has been inconsistent. To characterize the contribution of the host immune system to the potential for PERV transmission from pig islet tissue xenografts to host tissues, we examined two immunoincompetent animal models, thymectomizsed fetal lambs and NODscid mice. Pig proislets were grafted into fetal lambs or adult NODscid mice. Conventional, nested and real-time PCR/RT-PCR tests were used to search for PERV and pig cell-specific sequences (porcine mitochondrial cytochrome oxidase II (COII) or mitochondrial ribosomal 12S) in pig proislets, host liver and spleen at 5-84 days (lambs) or 96 days (mice) after transplantation. Xenografts were harvested at the same time points. The copy number of PERV sequences and host cell-specific nuclear (palmitoylcarnitine transferase) sequences was assessed by real-time PCR to estimate the proportion of PERV-infected host cells. Pig proislets were shown to be PERV+ve by PCR and immunohistochemistry (PERV B env protein p15E). PERV transmission (PERV A, B or C DNA in the absence of porcine COII or 12S sequences) was detected by nested PCR and real-time PCR in 4/12 fetal lamb liver samples 5-23 days after transplantation; the maximum copy number of PERV B env sequences was found at day 5 (700 copies/1 x 10(6) lamb cells). A total of 4/12 fetal lambs demonstrated both PERV and 12S porcine sequences in liver samples (days 5-84) by real-time PCR, suggesting that pig cells had migrated to those tissues and established microchimerism; nested PCR showed evidence for microchimerism (porcine COII sequences alone) in 2/12 lambs (day 5). The incidence of PERV transmission and frequency of microchimerism was similar in host spleen analysed by real-time PCR. Histological examination showed complete xenograft rejection by 23 days after transplantation to fetal lambs. In contrast, pig proislet xenografts survived long term (> or =day 96) in NODscid mice but no PERV transmission was found. Both nested and real-time PCR assays revealed that 2/3 mice had become microchimeric. Long-term expression of PERV A, B and C as well as porcine 12S or COII RNAs was found at the graft site (day 96) only, indicating that PERV transcription and possibly replication, continued in the donor pig islet tissue after transplantation. Overall, detection of PERV transmission and microchimerism was limited by the sensitivity of the PCR assay and the primers chosen. The absence of stable PERV transmission and microchimerism in fetal lambs and the rejection of pig proislet xenografts correlated in time with the establishment of host immunocompetence. We therefore suggest that the frequent failure to identify PERV transmission late after transplantation could be due to the immunological destruction of PERV-infected host cells. Recipient NODscid mice demonstrated long-term microchimerism and intragraft PERV expression, which was consistent with their stable immunoincompetence.
authors.affiliation: Division of Immunology and Genetics, John Curtin School of Medical Research, Australian National University, Canberra, Australian Capital Territory, Australia.
authors.forename: Sarah K
authors.initials: SK
authors.lastname: Popp
authors.forename: David A
authors.initials: DA
authors.lastname: Mann
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Milburn
authors.forename: Adrian J
authors.initials: AJ
authors.lastname: Gibbs
authors.forename: Peter J
authors.initials: PJ
authors.lastname: McCullagh
authors.forename: James Dennis
authors.initials: JD
authors.lastname: Wilson
authors.forename: Ralf R
authors.initials: RR
authors.lastname: Tönjes
authors.forename: Charmaine J
authors.initials: CJ
authors.lastname: Simeonovic
mesh_terms: D000818:Animals; D002460:Cell Line; D018076:DNA, Complementary; D003576:Electron Transport Complex IV / Q000235:genetics; D020077:Endogenous Retroviruses / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D005333:Fetus; D007108:Immune Tolerance; D016381:Islets of Langerhans Transplantation; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D008928:Mitochondria / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D034561:Sheep, Domestic / Q000196:embryology / Q000276:immunology / Q000601:surgery / Q000821:virology; D013154:Spleen / Q000378:metabolism; D034421:Sus scrofa / Q000821:virology*; D014183:Transplantation, Heterologous
chemical_list: D018076:DNA, Complementary; D012335:RNA, Ribosomal; C075526:RNA, ribosomal, 12S; D003576:Electron Transport Complex IV