title: HPLC analysis of allicin and other thiosulfinates in garlic clove homogenates.
abstract: Reversed-phase high Performance liquid chromatography (C18-HPLC) was used to separate and quantitate all the detectable alkyl and alkenyl thiosulfinates, including configurational isomers, of garlic homogenates. Pure thiosulfinates were synthesized or isolated and identified by (1)H-NMR, and their extinction coefficients determined. Some configurational isomers required Separation by silica-HPLC. Five previously unreported thiosulfinates have been found, four of which contain the TRANS-1-propenyl group and increase several-fold to over half the content of allicin upon storage of garlic bulbs at 4 degrees C with a concomitant decrease in a gamma-glutamyl peptide. The variation in thiosulfinate yield between different countries, stores, bulbs, cloves, and storage times was investigated. A method for standardizing the quantitation of allicin yield from garlic is proposed and compared to other methods of allicin analysis.
authors.affiliation: Murdock Healthcare, Springville, Utah 84663, U.S.A.
authors.forename: L D
authors.initials: LD
authors.lastname: Lawson
authors.forename: S G
authors.initials: SG
authors.lastname: Wood
authors.forename: B G
authors.initials: BG
authors.lastname: Hughes