Senescence in cultured trabecular meshwork cells.
BACKGROUND
It has been suggested that replicative senescence might be involved in the pathophysiology of age-related diseases.


OBJECTIVE
To study the process of senescence in trabecular meshwork (TM) cells.


METHODS
Porcine TM tissues were obtained and placed in primary cultures with Dulbecco's modified Eagle's medium/Ham's F-12 medium. After 2-3 weeks, migrated and proliferated TM cells were trypsinised and cultured in serial passages, and identified with fluorescein-labelled low-density lipoprotein (DiI-Ac-LDL), a marker of TM cells. Staining for senescence-related beta-galactosidase activity was performed at population doubling level (PDL) 2, 8 and 16 at pH 6. Terminal restriction fragment (TRF) length was examined by Southern blot analysis using a (32)P-labelled telomere-specific sequence (TTAGGG)(3) at each PDL.


RESULTS
DiI-Ac-LDL staining revealed that most (nearly 100%) of the cells in the culture were TM cells, which were flattened in shape and positive for senescence-related beta-galactosidase staining at PDL 16. Reduction of TRF length as a function of population doubling was also shown.


CONCLUSIONS
TM cells exhibited characteristics of senescence at PDL 16 in vitro. The results demonstrated that cellular senescence may be related to the pathophysiology of primary open-angle glaucoma.
Yamazaki
Yukari
Y
Department of Ophthalmology, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi, Osaka 570-8507, Japan.
Matsunaga
Hiroshi
H
Nishikawa
Maki
M
Ando
Akira
A
Kaneko
Shiho
S
Okuda
Koji
K
Wada
Mitsumasa
M
Ito
Seiji
S
Matsumura
Miyo
M
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D048430:Cell Shape; D016922:Cellular Senescence; D008077:Lipoproteins, LDL / Q000378:metabolism; D012150:Polymorphism, Restriction Fragment Length; D013552:Swine; D016615:Telomere; D046509:Tissue Culture Techniques; D014129:Trabecular Meshwork / Q000166:cytology* / Q000378:metabolism; D015854:Up-Regulation; D001616:beta-Galactosidase / Q000378:metabolism
D008077:Lipoproteins, LDL; D001616:beta-Galactosidase