title: [Simultaneous determination of three active constituents in cortex moutan by RP-HPLC].
abstract: OBJECTIVE
  To establish a RP-HPLC method for the simultaneous determination of gallic acid, paeoniflorin and paeonol in Cortex Moutan.
  
  
  METHODS
  The analysis was carried out on a column of YMC-C18 (4. 6 mm x 250 mm, 5 microm), and gradient elution was used for the baseline separation of these three constituents with the different detection wavelength.
  
  
  RESULTS
  The linear ranges for gallic acid, paeoniflorin and paeonol were 0.0130-0.267, 0.0880-1.76 and 0.164-3.28 microg, respectively. Their average recoveries were 104.3%, 101.7% and 99.5%, respectively.
  
  
  CONCLUSIONS
  The method was successfully applied to the determination of gallic acid, paeoniflorin and paeonol in Cortex Moutan from the different drugstores. It was shown in this study that the proposed method could be used to evaluate the quality of the medicinal plants or herbal preparations containing gallic acid, paeoniflorin and paeonol.
authors.affiliation: Department of Chinese Medicine Science and Engineering, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310027, China.
authors.forename: Yu-Wen
authors.initials: YW
authors.lastname: Wang
authors.forename: Ke
authors.initials: K
authors.lastname: Yu
authors.forename: Yi-Yu
authors.initials: YY
authors.lastname: Cheng
mesh_terms: D000098:Acetophenones / Q000032:analysis*; D001565:Benzoates / Q000032:analysis*; D001952:Bridged-Ring Compounds / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005707:Gallic Acid / Q000032:analysis*; D005960:Glucosides / Q000032:analysis*; D039821:Monoterpenes; D029596:Paeonia / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D006027:Glycosides
chemical_list: D000098:Acetophenones; D001565:Benzoates; D001952:Bridged-Ring Compounds; D004365:Drugs, Chinese Herbal; D005707:Gallic Acid; D005960:Glucosides; D039821:Monoterpenes; C038881:moutan cortex; C015423:peoniflorin; D006027:Glycosides; C013638:paeonol