title: Instability of miRNA and cDNAs derivatives in RNA preparations.
abstract: Micro RNAs (miRNAs) are small RNA molecules, which function as important regulators of gene expression. We found that RNA preparation methods commonly utilized for miRNA expression studies yield highly unstable miRNAs. We studied the stability of four miRNAs belonging to different miRNAs families. A significant degradation of these molecules may be observed already three days after RNA isolation. Moreover, the respective cDNAs are highly unstable as well. Our findings indicate that instability of miRNAs and their cDNAs should be considered when designing miRNA expression studies.
authors.affiliation: Diabetes Research Institute, University of Miami Leonard M. Miller School of Medicine, 1450 NW 10th Avenue, Miami, FL 33136, USA.
authors.forename: Valia
authors.initials: V
authors.lastname: Bravo
authors.forename: Samuel
authors.initials: S
authors.lastname: Rosero
authors.forename: Camillo
authors.initials: C
authors.lastname: Ricordi
authors.forename: Ricardo L
authors.initials: RL
authors.lastname: Pastori
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D018076:DNA, Complementary / Q000378:metabolism*; D015870:Gene Expression; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D020871:RNA Stability; D013997:Time Factors
chemical_list: D018076:DNA, Complementary; D035683:MicroRNAs; D012313:RNA