title: In vitro peroxynitrite scavenging activity of 6-hydroxykynurenic acid and other flavonoids from Gingko biloba yellow leaves.
abstract: As part of our research on phytochemicals that exert protective effects against diseases related to reactive nitrogen species, we have evaluated the scavenging activity of the yellow leaves of Ginkgo biloba on ONOO-. The methanol extract and ethyl acetate fraction obtained from yellow leaves of G. biloba evidenced a marked scavenging activity on authentic ONOO-. Repeated column chromatography of the active ethyl acetate soluble fraction on silica gel, Sephadex LH-20, and RP-18, resulted in the purification of 15 known compounds, including sciadopitysin (1), ginkgolide B (2), bilobalide (3), isoginkgetin (4), kaempferol (5), luteolin (6), protocatechuic acid (7), bilobetin (8), amentoflavone (9), beta-sitosterol glucopyranoside (10), kaempferol 3-O-rhamnopyranoside (11), kaempferol 3-O-glucopyranoside (12), kaempferol 3-O-[6"'-O-p-coumaroyl-beta-D-glucopyranosyl(1 --> 2)-alpha-L-rhamnopyranoside] (13), kaempferol 3-O-rutinoside (14), and 6-hydroxykynurenic acid (15). Among the compounds isolated, flavonoids (5, 6 and 11-14), protocatechuic acid (7), and 6-hydroxykynurenic acid (15) all exhibited marked scavenging activities on authentic ONOO-. The IC50 values of 5-7, 11-14 and 15 were as follows: 2.86 +/- 0.70, 2.30 +/- 0.04, 2.85 +/- 0.10, 5.60 +/- 0.47, 4.16 +/- 1.65, 2.47 +/- 0.15, 3.02 +/- 0.48, and 6.24 +/- 0.27 microM, respectively. DL-Penicillamine (IC50 = 4.98 +/- 0.27 microM) was utilized as a positive control. However, the other compounds (1-4, 8-10) exerted no effects against ONOO-.
authors.affiliation: Faculty of Food Science and Biotechnology, Pukyong National University, Busan 608-737, Korea.
authors.forename: Sook Kyung
authors.initials: SK
authors.lastname: Hyun
authors.forename: Hyun Ah
authors.initials: HA
authors.lastname: Jung
authors.forename: Hae Young
authors.initials: HY
authors.lastname: Chung
authors.forename: Jae Sue
authors.initials: JS
authors.lastname: Choi
mesh_terms: D000431:Ethanol; D005419:Flavonoids / Q000737:chemistry*; D016166:Free Radical Scavengers; D020441:Ginkgo biloba / Q000737:chemistry*; D007736:Kynurenic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D000432:Methanol; D009569:Nitric Oxide / Q000737:chemistry; D016877:Oxidants / Q000737:chemistry; D030421:Peroxynitrous Acid / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D012997:Solvents; D013481:Superoxides / Q000737:chemistry
chemical_list: C428563:6-hydroxykynurenic acid; D005419:Flavonoids; D016166:Free Radical Scavengers; D016877:Oxidants; D012997:Solvents; D013481:Superoxides; D030421:Peroxynitrous Acid; D009569:Nitric Oxide; D000431:Ethanol; D007736:Kynurenic Acid; D000432:Methanol