Fold-recognition and comparative modeling of human beta3GalT I, II, IV, V and VI and beta3GalNAcT I: prediction of residues conferring acceptor substrate specificity.
beta3GalTs are type II transmembrane proteins that transfer galactose from UDP-Gal donor substrate to acceptor GlcNAc, GalNAc or Gal in beta1-->3-linkage. beta1-->3-linked galactose have been found to be a part of many glycans like glycosphingolipids, core tetrasaccharide of proteoglycans, type 1 chains. The 3-D structure of none of the beta3GalTs is known to date. In this study, the 3-D structures of human beta3GalT I, II, IV, V, VI and beta3GalNAcT I have been modeled using fold-recognition and comparative modeling methods. Residues that constitute the UDP-Gal binding site have been predicted. The models are able to qualitatively rationalize data from the site-directed mutagenesis experiments reported in the literature. Residues likely to be involved in conferring differential acceptor substrate specificity have been predicted by a combination of specificity determining positions prediction (SDPs) and subsequent mapping on the generated 3-D models.
Patel
Ronak Y
RY
School of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
Balaji
Petety V
PV
D000595:Amino Acid Sequence; D000818:Animals; D002240:Carbohydrate Sequence; D020134:Catalytic Domain; D003198:Computer Simulation; D005700:Galactosyltransferases / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017350:N-Acetylgalactosaminyltransferases / Q000737:chemistry* / Q000378:metabolism; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017386:Sequence Homology, Amino Acid; D012984:Software; D013379:Substrate Specificity
D009844:Oligosaccharides; C479886:B3GALT5 protein, human; C425302:B3GALT6 protein, human; D005700:Galactosyltransferases; D017350:N-Acetylgalactosaminyltransferases