title: A population of myogenic stem cells that survives skeletal muscle aging.
abstract: Age-related decline in integrity and function of differentiated adult tissues is widely attributed to reduction in number or regenerative potential of resident stem cells. The satellite cell, resident beneath the basal lamina of skeletal muscle myofibers, is the principal myogenic stem cell. Here we have explored the capacity of satellite cells within aged mouse muscle to regenerate skeletal muscle and to self-renew using isolated myofibers in tissue culture and in vivo. Satellite cells expressing Pax7 were depleted from aged muscles, and when aged myofibers were placed in culture, satellite cell myogenic progression resulted in apoptosis and fewer total differentiated progeny. However, a minority of cultured aged satellite cells generated large clusters of progeny containing both differentiated cells and new cells of a quiescent satellite-cell-like phenotype characteristic of self-renewal. Parallel in vivo engraftment assays showed that, despite the reduction in Pax7(+) cells, the satellite cell population associated with individual aged myofibers could regenerate muscle and self-renew as effectively as the larger population of satellite cells associated with young myofibers. We conclude that a minority of satellite cells is responsible for adult muscle regeneration, and that these stem cells survive the effects of aging to retain their intrinsic potential throughout life. Thus, the effectiveness of stem-cell-mediated muscle regeneration is determined by both extrinsic environmental influences and diversity in intrinsic potential of the stem cells themselves.
authors.affiliation: The Dubowitz Neuromuscular Unit, Department of Paediatrics, Imperial College London, Hammersmith Hospital, London, U.K.
authors.forename: Charlotte A
authors.initials: CA
authors.lastname: Collins
authors.forename: Peter S
authors.initials: PS
authors.lastname: Zammit
authors.forename: Ana Pérez
authors.initials: AP
authors.lastname: Ruiz
authors.forename: Jennifer E
authors.initials: JE
authors.lastname: Morgan
authors.forename: Terence A
authors.initials: TA
authors.lastname: Partridge
mesh_terms: D000375:Aging; D000818:Animals; D018929:Cell Culture Techniques; D002470:Cell Survival; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000502:physiology / Q000637:transplantation; D018482:Muscle, Skeletal / Q000166:cytology* / Q000254:growth & development / Q000293:injuries; D051763:PAX7 Transcription Factor / Q000502:physiology; D013234:Stem Cells / Q000166:cytology*
chemical_list: D051763:PAX7 Transcription Factor; C497321:PAX7 protein, human