Transposon-tagged mutagenesis in the rat.
Although the laboratory rat (Rattus norvegicus) is an indispensable experimental animal for biomedical research and drug development, the lack of embryonic stem cell lines hampers gene-knockout studies. Here we report the successful generation of insertional mutant rats using the Sleeping Beauty (SB) transposon system. This would benefit a variety of biomedical research fields for which the rat model is better suited than the mouse model.
Kitada
Kazuhiro
K
Laboratory of Mammalian Genetics, Division of Genome Dynamics, Creative Research Initiative Sousei, Hokkaido University, North 10 West 8, Kita-ku, Sapporo 060-0810, Japan. kkitada@cast.hokudai.ac.jp
Ishishita
Satoshi
S
Tosaka
Keiko
K
Takahashi
Ri-ichi
R
Ueda
Masatsugu
M
Keng
Vincent W
VW
Horie
Kyoji
K
Takeda
Junji
J
D000251:Adenosine Triphosphatases / Q000235:genetics; D000818:Animals; D030801:Animals, Genetically Modified; D004251:DNA Transposable Elements; D005260:Female; D005804:Genes, Lethal; D049452:Green Fluorescent Proteins / Q000235:genetics; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D006720:Homozygote; D008297:Male; D051379:Mice; D023421:Models, Animal; D016254:Mutagenesis, Insertional; D009419:Nerve Tissue Proteins / Q000235:genetics; D051381:Rats / Q000235:genetics*; D011971:Receptors, Immunologic / Q000235:genetics; D019895:Transposases / Q000235:genetics; D000097905:Roundabout Proteins
D000251:Adenosine Triphosphatases; D004251:DNA Transposable Elements; D049452:Green Fluorescent Proteins; D018840:HSP70 Heat-Shock Proteins; D009419:Nerve Tissue Proteins; D011971:Receptors, Immunologic; D019895:Transposases; C516057:Robo2 protein, rat; D000097905:Roundabout Proteins; C517966:Hspa13 protein, rat