title: [Determination of acacetin in Xiangjuganmao Keli (no sweet) by HPLC].
abstract: OBJECTIVE
  To establish a method for the determination of acacetin in Xiangjuganmao Keli (no sweet).
  
  
  METHODS
  Acacetin in powdered herb was extracted by ultrasonator with methanol and was hydrolyzed with hydrochloric acid. Separation was accomplished on an ODS reversed phase column (5 microm, 4.6 x 250 mm) with a mobile phase of methanol-water-acetic acid(350: 150: 2). The detective wavelength was at 340 nm.
  
  
  RESULTS
  The method was accurate, the results were stable and reproducible. The linear range of calibration cure was within the concentration of 2.00 - 10.00 microg/ml (r = 0.9998). The average extraction recovery was 99.9% (n = 6), RSD = 0.41% (n = 6).
  
  
  CONCLUSIONS
  The method is simple, convenient, sensitive, and reproducible for quality control of Xiangjuganmao Keli (no sweet).
authors.affiliation: Leiyunshang Medical Co. Ltd., Suzhou 215009, China.
authors.forename: Jia-Hong
authors.initials: JH
authors.lastname: Bian
authors.forename: Kun
authors.initials: K
authors.lastname: Qian
authors.forename: Xiang
authors.initials: X
authors.lastname: Xu
authors.forename: Jun
authors.initials: J
authors.lastname: Shen
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D047309:Flavones / Q000032:analysis* / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D013997:Time Factors
chemical_list: D004365:Drugs, Chinese Herbal; D047309:Flavones; C023717:acacetin