title: Differential antigen processing by dendritic cell subsets in vivo.
abstract: Dendritic cells (DCs) process and present self and foreign antigens to induce tolerance or immunity. In vitro models suggest that induction of immunity is controlled by regulating the presentation of antigen, but little is known about how DCs control antigen presentation in vivo. To examine antigen processing and presentation in vivo, we specifically targeted antigens to two major subsets of DCs by using chimeric monoclonal antibodies. Unlike CD8+ DCs that express the cell surface protein CD205, CD8- DCs, which are positive for the 33D1 antigen, are specialized for presentation on major histocompatibility complex (MHC) class II. This difference in antigen processing is intrinsic to the DC subsets and is associated with increased expression of proteins involved in MHC processing.
authors.affiliation: Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10021, USA.
authors.forename: Diana
authors.initials: D
authors.lastname: Dudziak
authors.forename: Alice O
authors.initials: AO
authors.lastname: Kamphorst
authors.forename: Gordon F
authors.initials: GF
authors.lastname: Heidkamp
authors.forename: Veit R
authors.initials: VR
authors.lastname: Buchholz
authors.forename: Christine
authors.initials: C
authors.lastname: Trumpfheller
authors.forename: Sayuri
authors.initials: S
authors.lastname: Yamazaki
authors.forename: Cheolho
authors.initials: C
authors.lastname: Cheong
authors.forename: Kang
authors.initials: K
authors.lastname: Liu
authors.forename: Han-Woong
authors.initials: HW
authors.lastname: Lee
authors.forename: Chae Gyu
authors.initials: CG
authors.lastname: Park
authors.forename: Ralph M
authors.initials: RM
authors.lastname: Steinman
authors.forename: Michel C
authors.initials: MC
authors.lastname: Nussenzweig
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D017951:Antigen Presentation; D015703:Antigens, CD / Q000032:analysis / Q000276:immunology; D001483:Base Sequence; D016827:CD8 Antigens / Q000032:analysis / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D037181:Lectins, C-Type / Q000032:analysis / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D015778:Minor Histocompatibility Antigens; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D011956:Receptors, Cell Surface / Q000032:analysis / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D016827:CD8 Antigens; C093569:DEC-205 receptor; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D037181:Lectins, C-Type; D015778:Minor Histocompatibility Antigens; D011956:Receptors, Cell Surface