Establishment and biochemical characterization of primary cells of the upper aerodigestive tract.
The upper aerodigestive tract, composed of the oral cavity, the pharynx and the esophagus, is a complex system whose components function in both organ-specific ways as well in serving as a protective barrier against the enzymes which initiate digestion as well as against the mechanical functions which serve to ensure movement of food through the upper aerodigestive tract. Given these diverse functional requirements, the study of the anatomy and physiology of this region are uniquely complex and significantly understudied. The goal of the current study was to develop a simple and reproducible method for the isolation, growth, and maintenance of primary epithelial cells from the oral cavity, the pharynx and the esophagus. In addition, given the increased interest in diseases characterized by a loss of mucosal integrity in these areas which is often accompanied by a diminished wound healing capability, these cells were biochemically characterized with a focus on the components of the extracellular matrix remodeling axis including the activity and inhibition of the matrix metalloproteinases (MMPs).
Rickert
Dorothee
D
Department of Otolaryngology and Head and Neck Surgery, University of Ulm, Prittwitzstrasse 43, 89075 Ulm, Germany. Dorothee.Rickert@uniklinik-ulm.de
Franke
R-P
RP
Fernández
Cecilia A
CA
Kilroy
Susan
S
Yan
Li
L
Moses
Marsha A
MA
D000199:Actins / Q000032:analysis; D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002478:Cells, Cultured / Q000737:chemistry / Q000166:cytology / Q000648:ultrastructure; D004847:Epithelial Cells / Q000737:chemistry / Q000166:cytology* / Q000648:ultrastructure; D004947:Esophagus / Q000166:cytology*; D005109:Extracellular Matrix / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D005780:Gelatin; D007633:Keratins / Q000032:analysis; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000032:analysis; D020778:Matrix Metalloproteinase 2 / Q000032:analysis; D009061:Mouth Mucosa / Q000166:cytology*; D009092:Mucous Membrane / Q000166:cytology; D010614:Pharynx / Q000166:cytology*; D010969:Plastics; D051381:Rats; D017207:Rats, Sprague-Dawley; D019714:Tissue Inhibitor of Metalloproteinases / Q000032:analysis
D000199:Actins; D005780:Gelatin; D007633:Keratins; D020781:Matrix Metalloproteinase 1; D020778:Matrix Metalloproteinase 2; D010969:Plastics; D019714:Tissue Inhibitor of Metalloproteinases; C498862:Acta2 protein, rat