PD-L1 expression analysis in gastric carcinoma tissue and blocking of tumor-associated PD-L1 signaling by two functional monoclonal antibodies.
Programmed death-1 ligand-1 (PD-L1), a member of the B7 family of costimulatory molecules, plays an important role in the regulations of the cellular and humoral immune responses. In this study, two mouse anti-human PD-L1 monoclonal antibodies named 10E10 and 2H11 were successfully generated and further characterized. Monoclonal antibody 10E10 bound to distinct PD-L1 epitope comparing an available anti-PD-L1 monoclonal antibody on a series of malignant cell lines, activated T lymphocytes, B lymphocytes and dendritic cells. Then, by using immunohistochemistry staining with monoclonal antibody 2H11, the expression of PD-L1 was found in human gastric carcinoma specimens but not in normal or gastric adenoma tissues. Additional data show that PD-L1 can be regarded as a decisive factor in evaluating gastric carcinoma prognosis and anti-human PD-L1 monoclonal antibody 10E10 could inhibit T-cell apoptosis induced by tumor-associated PD-L1. Taken together, these results showed that the two functional mouse anti-human PD-L1 monoclonal antibodies we generated might be of great value for further exploration of the costimulatory molecule regulating network and immunointervention for tumor immunotherapy.
Sun
J
J
Medical Biotechnology Institute, and Stem Cell Research Laboratory of Jiangsu Province, Soochow University, Suzhou, 215007, China.
Xu
K
K
Wu
C
C
Wang
Y
Y
Hu
Y
Y
Zhu
Y
Y
Chen
Y
Y
Shi
Q
Q
Yu
G
G
Zhang
X
X
D000818:Animals; D019138:Antibodies, Blocking / Q000502:physiology*; D000911:Antibodies, Monoclonal / Q000502:physiology*; D015703:Antigens, CD / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology* / Q000502:physiology; D060890:B7-H1 Antigen; D002277:Carcinoma / Q000276:immunology / Q000378:metabolism*; D045744:Cell Line, Tumor; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D015398:Signal Transduction / Q000276:immunology*; D013274:Stomach Neoplasms / Q000276:immunology / Q000378:metabolism*
D019138:Antibodies, Blocking; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D060890:B7-H1 Antigen; C423236:CD274 protein, human