[Research of polysaccharide from Dioscorea opposita Thunb].
OBJECTIVE
To isolate a homogeneous polysaccharide RP from RDP (a crude polysaccharide from Dioscorea opposita Thunb.) , and study its preliminary composition and structure.


METHODS
RP was obtained with water extraction, alcohol sedimentation, CTAB deprotein, cellulose column and SephadexG-100 column. The purities of RP were identified by SephadexG-200. PC analysis on its acidic hydrolysates was used to determine the sugar components. Sephadex chromatography was used to mensurate its molecular weight. IR was used to analysis RP.


RESULTS
RP was homogeneous. IR indicated that RP had beta configurations. The compositon was identified by paper chromatography as glucose, D-mannose, D-galactose.


CONCLUSIONS
The research could provide a theoretical foundation for further development and utilization.
Xu
Qin
Q
Jiangsu Instiute of Botany, Chinese Academy of Sciences, Nanjing Botanical Garden, Nanjing 210014, China.
Xu
Zeng-lai
ZL
Shen
Zhen-guo
ZG
Shi
Yun-yun
YY
D027743:Dioscoreaceae / Q000737:chemistry*; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D008358:Mannose / Q000032:analysis; D008970:Molecular Weight; D035281:Plant Tubers / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
D011134:Polysaccharides; D005947:Glucose; D008358:Mannose; D005690:Galactose