title: HSP90-like artificial chaperone activity based on indole beta-cyclodextrin.
abstract: Indole beta-cyclodextrin (beta-1) was found to be able to prevent aggregation of citrate synthase (CS) on heating condition. As a result, beta-1 showed anti-CS aggregation in this system by regulating in early stage. The depression mechanism of beta-1 for aggregation of CS is as follows: the beta-1 formed a complex with hydrophobic parts of the beta-sheet structure of CS. From CD spectra, CS was changed own conformation was changed by beta-1 addition. So, it was concluded that beta-1 works as beta-sheet inducer in thermal condition. On the other hand, native beta-cyclodextrin (beta-CyD) shows small suppression capability for CS aggregation.
authors.affiliation: Department of Material-Process Engineering and Applied Chemistry for Environments, Faculty of Engineering and Resource Science, Akita University, Tegata, Akita 010-8502, Japan.
authors.forename: Masaya
authors.initials: M
authors.lastname: Toda
authors.forename: Hideaki
authors.initials: H
authors.lastname: Itoh
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Kondo
authors.forename: Fumio
authors.initials: F
authors.lastname: Hamada
mesh_terms: D002942:Circular Dichroism; D018841:HSP90 Heat-Shock Proteins / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D013056:Spectrophotometry, Ultraviolet; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D018841:HSP90 Heat-Shock Proteins; D007211:Indoles; D047392:beta-Cyclodextrins; C031215:betadex