title: In vitro biodegradation and biocompatibility of gelatin/montmorillonite-chitosan intercalated nanocomposite.
abstract: The intercalated nanocomposite of gelatin/montmorillonite-chitosan (Gel/MMT-CS) was prepared via the solution intercalation process. In vitro degradation tests showed that the nanocomposite had a lower degradation rate than Gel-CS composite. And the introduced intercalation structure endowed Gel/MMT-CS nanocomposite with a controllable degradation rate when changing the MMT content. Cells attachment, spread and proliferation on the Gel/MMT-CS membranes were investigated by scanning electron microscopy (SEM) and mitochondrial activity assay. The results provided evidences of good adhesion, proliferation and morphology of rat stromal stem cells on Gel/MMT-CS membranes compared to the tissue culture plates (TCPs), making the Gel/MMT-CS nanocomposite a promising candidate towards tissue engineering.
authors.affiliation: College of Materials Science and Engineering, Tianjin University, Tianjin, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhuang
authors.forename: Jun Ping
authors.initials: JP
authors.lastname: Zheng
authors.forename: Hong
authors.initials: H
authors.lastname: Gao
authors.forename: Kang
authors.initials: K
authors.lastname: De Yao
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001546:Bentonite / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry* / Q000633:toxicity; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry* / Q000633:toxicity; D005780:Gelatin / Q000737:chemistry* / Q000633:toxicity; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D053761:Nanocomposites / Q000737:chemistry / Q000633:toxicity; D051381:Rats; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D023822:Tissue Engineering; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D001546:Bentonite; D005780:Gelatin; D048271:Chitosan