The interactions of aurein 1.2 with cancer cell membranes.
Here, the interactions of aurein 1.2, a defence peptide, with T98G glioblastoma cell membranes are studied. The peptide induced maximal surface pressure changes of circa 9 mN m(-1) in monolayers of endogenous T98G membrane lipid. Reducing monolayer anionic lipid showed a positive correlation (R(2)>0.91) with decreases in maximal surface pressure changes induced by aurein 1.2 (circa 3 mN m(-1) in the absence of this lipid). Cancer cell membrane invasion by the peptide therefore appears not to be mediated by lipid receptors or specific lipid requirements but rather a general requirement for anionic lipid and/or other negatively charged membrane components.
Dennison
Sarah R
SR
Faculty of Science and Technology, University of Central Lancashire, Preston PR1 2HE, UK.
Harris
Frederick
F
Phoenix
David A
DA
D023181:Antimicrobial Cationic Peptides / Q000737:chemistry* / Q000378:metabolism; D001932:Brain Neoplasms / Q000737:chemistry* / Q000378:metabolism; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D005909:Glioblastoma / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008563:Membrane Lipids / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D011487:Protein Conformation
D023181:Antimicrobial Cationic Peptides; D008563:Membrane Lipids; C506635:aurein 1.2 peptide