title: Blood flow occlusion via ultrasound image-guided high-intensity focused ultrasound and its effect on tissue perfusion.
abstract: This study investigated the induction of tissue necrosis by arterial blood flow occlusion using ultrasound image-guided high-intensity focused ultrasound (HIFU). We constructed a prototype HIFU transducer in combination with an imaging probe that provided color Doppler imaging and ultrasound contrast imaging. The HIFU beam was aimed into a branch of the renal artery in vivo. The renal artery branches of eight rabbits were occluded by HIFU at an intensity of 4 kW/cm(2) (from 2 to 10 times of each sonication for 5 s). When the HIFU exposure was successful, complete cessation of blood flow was observed by color Doppler imaging with success rate of 100% (8/8). Furthermore, lack of perfusion was observed in the renal cortex with a contrast-enhanced image. Postmortem histologic evaluation showed a wedge-shaped area of infarction in six of seven cases, corresponding to the lack of the contrast medium in the ultrasound image. These results demonstrated that ultrasound image-guided HIFU can be used to induce arterial occlusion, thus producing infarction and necrosis of the perfused tissue.
authors.affiliation: Department of Obstetrics and Gynecology, Showa University School of Medicine, Shinagawa, Tokyo, Japan. m_itihara@hotmail.com
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Ichihara
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Sasaki
authors.forename: Shin-ichiro
authors.initials: S
authors.lastname: Umemura
authors.forename: Miki
authors.initials: M
authors.lastname: Kushima
authors.forename: Takashi
authors.initials: T
authors.lastname: Okai
mesh_terms: D000418:Albumins / Q000008:administration & dosage; D000818:Animals; D003287:Contrast Media / Q000008:administration & dosage; D005260:Female; D005466:Fluorocarbons / Q000008:administration & dosage; D007275:Injections, Intravenous; D007668:Kidney / Q000098:blood supply / Q000473:pathology*; D007672:Kidney Cortex / Q000473:pathology; D045423:Microbubbles; D009336:Necrosis; D011817:Rabbits; D012077:Renal Artery / Q000000981:diagnostic imaging / Q000473:pathology; D012079:Renal Circulation / Q000502:physiology*; D014464:Ultrasonic Therapy / Q000379:methods*; D018615:Ultrasonography, Doppler, Color / Q000379:methods*
chemical_list: D000418:Albumins; D003287:Contrast Media; C099458:FS 069; D005466:Fluorocarbons