Synthesis and serological interactions of H. pylori urease fragment 321-339 n-terminally immobilized on the cellulose.
Antigenic epitopes F8 (SIKEDVQF) and UB-33 (UreB fragment with residues 321-339: CHHLDKSIKEDVQFADSRI) in H. pylori urease that induces neutralizing antibody production were prepared on the cellulose plate from N- to C-terminus using CDMT as a coupling reagent. Reaction of both epitopes with sera of patients with medically confirmed atherosclerosis was studied. Strong, selective reactions of both peptides with some patients sera were observed.
Kolesińska
Beata
B
Institute of Organic Chemistry, łódź University of Technology, Zeromskiego 116, 90-924 łódź, Poland.
Grabowski
Sebastian
S
Kaca
Wiesław
W
Kamiński
Zbigniew J
ZJ
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D015153:Blotting, Western; D002482:Cellulose; D004800:Enzymes, Immobilized; D000939:Epitopes / Q000276:immunology; D016480:Helicobacter pylori / Q000201:enzymology*; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000737:chemistry; D014510:Urease / Q000138:chemical synthesis* / Q000737:chemistry
D000911:Antibodies, Monoclonal; D004800:Enzymes, Immobilized; D000939:Epitopes; D010446:Peptide Fragments; D002482:Cellulose; D014510:Urease