title: Interleukin-1 superfamily genes expression in normal or impaired human spermatogenesis.
abstract: Interleukin-1 (IL-1) is a pleiotropic cytokine that may play a role in contributing to the specific immune environment of mammalian testis and in regulating cell differentiation. We have determined the transcription activity of the IL-1 gene family (using real-time polymerase chain reaction (PCR)) in two main functional testicular compartments (interstitial and intratubular ones), and in tissue homogenates obtained from patients with fertility disorders (spermatogenic arrest and testicular tumors). We observed the prominent expression of gene coding for IL-1 receptor antagonist (IL-1RA) in a purified fraction of gametogenic cells (normal gonad). Caspase-1 (ICE - IL-1beta-converting enzyme) was highly expressed (on mRNA level) in interstitial compartments as well in testicular tumors (immune enhancement?). In addition we found, that the activity of IL-1RA gene decreased along spermatogenic alteration in an inversely related manner with IL-1alpha (from normal gonad through spermatogenic arrest to Sertoli cell only syndrome). Therefore, the quotient value of IL-1alpha/IL-1RA could potentially serve as the diagnostic molecular probe for spermatogenesis assessment. The precise level of mRNA for IL-1-IL-18 cytokines and their receptors, and specifically of the receptor antagonist in immune privileged gonad, could be one of the main factors responsible for maintaining testicular homeostasis, thus enabling generation of the mature spermatozoa.
authors.affiliation: Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland.
authors.forename: N
authors.initials: N
authors.lastname: Rozwadowska
authors.forename: D
authors.initials: D
authors.lastname: Fiszer
authors.forename: P
authors.initials: P
authors.lastname: Jedrzejczak
authors.forename: W
authors.initials: W
authors.lastname: Kosicki
authors.forename: M
authors.initials: M
authors.lastname: Kurpisz
mesh_terms: D020170:Caspase 1 / Q000378:metabolism; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D007248:Infertility, Male / Q000235:genetics / Q000378:metabolism*; D053590:Interleukin 1 Receptor Antagonist Protein / Q000378:metabolism; D007375:Interleukin-1 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D016133:Polymerase Chain Reaction; D013091:Spermatogenesis / Q000235:genetics* / Q000502:physiology; D018709:Statistics, Nonparametric; D013737:Testis / Q000378:metabolism* / Q000473:pathology
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D020170:Caspase 1