title: Nestin expression in adult and developing human kidney.
abstract: Nestin is considered a marker of neurogenic and myogenic precursor cells. Its arrangement is regulated by cyclin-dependent kinase 5 (CDK5), which is expressed in murine podocytes. We investigated nestin expression in human adult and fetal kidney as well as CDK5 presence in adult human podocytes. Confocal microscopy demonstrated that adult glomeruli display nestin immunoreactivity in vimentin-expressing cells with the podocyte morphology and not in cells bearing the endothelial marker CD31. Glomerular nestin-positive cells were CDK5 immunoreactive as well. Western blotting of the intermediate filament-enriched cytoskeletal fraction and coimmunoprecipitation of nestin with anti-CDK5 antibodies confirmed these results. Nestin was also detected in developing glomeruli within immature podocytes and a few other cells. Confocal microscopy of experiments conducted with antibodies against nestin and endothelial markers demonstrated that endothelial cells belonging to capillaries invading the lower cleft of S-shaped bodies and the immature glomeruli were nestin immunoreactive. Similar experiments carried out with antibodies raised against nestin and alpha-smooth muscle actin showed that the first mesangial cells that populate the developing glomeruli expressed nestin. In conclusion, nestin is expressed in the human kidney from the first steps of glomerulogenesis within podocytes, mesangial, and endothelial cells. This expression, restricted to podocytes in mature glomeruli, appears associated with CDK5.
authors.affiliation: Dept. of Pharmacology Giorgio Segre, Section of Anatomy, University of Siena, Via Aldo Moro 4, I-53100 Siena, Italy. bertelli5@unisi.it
authors.forename: Eugenio
authors.initials: E
authors.lastname: Bertelli
authors.forename: Marì
authors.initials: M
authors.lastname: Regoli
authors.forename: Luciano
authors.initials: L
authors.lastname: Fonzi
authors.forename: Rossella
authors.initials: R
authors.lastname: Occhini
authors.forename: Susanna
authors.initials: S
authors.lastname: Mannucci
authors.forename: Leonardo
authors.initials: L
authors.lastname: Ermini
authors.forename: Paolo
authors.initials: P
authors.lastname: Toti
mesh_terms: D037881:Aborted Fetus / Q000737:chemistry*; D000328:Adult; D000368:Aged; D001344:Autopsy; D015153:Blotting, Western; D051360:Cyclin-Dependent Kinase 5 / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000032:analysis*; D007668:Kidney / Q000737:chemistry* / Q000166:cytology; D018613:Microscopy, Confocal; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000032:analysis*; D064231:Nestin; D050199:Podocytes / Q000378:metabolism
chemical_list: D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D051360:Cyclin-Dependent Kinase 5; C495905:CDK5 protein, human