title: Ganoderma lucidum polysaccharide peptide reduced the production of proinflammatory cytokines in activated rheumatoid synovial fibroblast.
abstract: The aim of the current study was to elucidate the potential therapeutic effect of Ganoderma lucidum polysaccharide peptide (GL-PP) in rheumatoid arthritis (RA). The effects of GL-PP on cell proliferation and cytokine production were studied in RA synovial fibroblasts (RASF). GL-PP significantly inhibited the proliferation of RASF. Following the incubation with GL-PP, production of interleukin (IL)-6 and monocyte chemoattractant protein (MCP)-1 in RASF were significantly increased as expressed as percentage change from basal values. However, the actual effects were minimal due to the low basal values. When RASF were activated by IL-1beta or lipopolysaccharides, IL-8 and MCP-1 production increased many folds. GL-PP significantly suppressed their productions. The inhibitory effects of GL-PP on cytokine production in RASF were at least in part, by inhibiting the nuclear factor-kappa B (NF-kappaB) transcription pathway. Our results demonstrated that GL-PP had the unique ability to modulate cytokine production in RASF and warrants further investigation into its mechanism of action.
authors.affiliation: Department of Pharmacology, University of Hong Kong, Hong Kong SAR, Hong Kong.
authors.forename: Y W
authors.initials: YW
authors.lastname: Ho
authors.forename: J S L
authors.initials: JS
authors.lastname: Yeung
authors.forename: P K Y
authors.initials: PK
authors.lastname: Chiu
authors.forename: W M
authors.initials: WM
authors.lastname: Tang
authors.forename: Z B
authors.initials: ZB
authors.lastname: Lin
authors.forename: R Y K
authors.initials: RY
authors.lastname: Man
authors.forename: C S
authors.initials: CS
authors.lastname: Lau
mesh_terms: D001172:Arthritis, Rheumatoid / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008516:Medicine, Chinese Traditional; D016328:NF-kappa B / Q000378:metabolism; D011509:Proteoglycans / Q000494:pharmacology / Q000627:therapeutic use; D020940:Reishi / Q000737:chemistry*; D013583:Synovial Membrane / Q000166:cytology*
chemical_list: D016207:Cytokines; D016328:NF-kappa B; D011509:Proteoglycans; C093216:polysaccharide peptide