[Screening of modified polyethylene surfaces for tissue engineering of chondrocytes].
BACKGROUND
Reconstructive surgery needs high requirements of substitutes for cartilages. The availability of autologous material is limited. The use of implants can cause inflammatory reactions. Therefore the biocompatibility of porous polyethylene should be improved by masking the synthetic surface with autologous cells. The polyethylene surface was functionalized by collagen, in order to enhance the contact between polyethylene and the surrounding cells.


METHODS
The modified surfaces were characterized and tested by an in vitro screening with primary human chondrocytes.


RESULTS
The modification of polyethylene surfaces by collagen coating increased the life time of chondrocytes growing at this surface. The effect was independent of the former functionalization.


CONCLUSIONS
It is possible to cultivate chondrocytes on polyethylene surfaces. The results have to be proven in a long-term animal experimental study.
Röpke
E
E
Klinik und Poliklinik für Hals-, Nasen-, Ohrenheilkunde, Kopf- und Halschirurgie, Martin-Luther-Universität Halle-Wittenberg, Halle/Saale. ernst.roepke@medizin.uni-halle.de
Schön
I
I
Vogel
J
J
Jamali
J
J
Bloching
M
M
Berghaus
A
A
D001672:Biocompatible Materials; D002448:Cell Adhesion / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D019902:Chondrocytes / Q000166:cytology*; D020099:Coated Materials, Biocompatible; D003094:Collagen / Q000378:metabolism; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009300:Nasal Septum / Q000601:surgery; D011095:Polyethylenes; D016062:Porosity; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; C084562:Medpor; D011095:Polyethylenes; D003094:Collagen