Identification of a novel bifunctional delta12/delta15 fatty acid desaturase from a basidiomycete, Coprinus cinereus TD#822-2.
A new gene encoding a delta12 fatty acid desaturase-related protein was cloned from a multicellular basidiomycete Coprinus cinereus TD#822-2. The 1326 bp full-length gene, designated as Cop-odeA, codes for a putative protein of 442 amino acids with a MW of 49224. The Cop-odeA yeast transformant accumulated four new fatty acids identified as 9,12-hexadecadienoic acid, 9,12,15-hexadecatrienoic acid, linoleic acid, and alpha-linolenic acid, which comprised 8.8%, 1.0%, 29.0%, and 0.6% of the total fatty acids, respectively. The Cop-odeA protein was confirmed to be a novel bifunctional fatty acid desaturase with both high delta12 desaturase activity and unusual delta15 desaturase activity.
Zhang
Shuo
S
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
Sakuradani
Eiji
E
Ito
Kuni
K
Shimizu
Sakayu
S
D003001:Cloning, Molecular; D003302:Coprinus / Q000201:enzymology* / Q000235:genetics; D044943:Fatty Acid Desaturases / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis / Q000096:biosynthesis; D010802:Phylogeny; D012441:Saccharomyces cerevisiae / Q000235:genetics
D005227:Fatty Acids; D044943:Fatty Acid Desaturases; C040448:delta-12 fatty acid desaturase