[Determination of acacetin in Xiangjuganmao Keli (no sweet) by HPLC].
OBJECTIVE
To establish a method for the determination of acacetin in Xiangjuganmao Keli (no sweet).


METHODS
Acacetin in powdered herb was extracted by ultrasonator with methanol and was hydrolyzed with hydrochloric acid. Separation was accomplished on an ODS reversed phase column (5 microm, 4.6 x 250 mm) with a mobile phase of methanol-water-acetic acid(350: 150: 2). The detective wavelength was at 340 nm.


RESULTS
The method was accurate, the results were stable and reproducible. The linear range of calibration cure was within the concentration of 2.00 - 10.00 microg/ml (r = 0.9998). The average extraction recovery was 99.9% (n = 6), RSD = 0.41% (n = 6).


CONCLUSIONS
The method is simple, convenient, sensitive, and reproducible for quality control of Xiangjuganmao Keli (no sweet).
Bian
Jia-Hong
JH
Leiyunshang Medical Co. Ltd., Suzhou 215009, China.
Qian
Kun
K
Xu
Xiang
X
Shen
Jun
J
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D047309:Flavones / Q000032:analysis* / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D013997:Time Factors
D004365:Drugs, Chinese Herbal; D047309:Flavones; C023717:acacetin