[Study on the preparation and quality standard of Ephedra sinica dispensing granules].
OBJECTIVE
To optimize the preparation process and to set up the quality standard of Ephedra sinica Dispensing Granules.


METHODS
Nine experiments were carried out by L9 (3(4)) orthogonal design. Ephedra sinica Dispensing Granules was identified by TLC and the content of ephedrine was determined by HPLC.


RESULTS
The optimal extraction process was as follows: the water that 10 times of Ephedra sinica, was decocted 3 times and each time was sustained for 1.5 hours. The linear relationship of ephedrine hydrochloride was at the range of 0.0081 - 0.1377 microg (r = 0.999 9). The average recovery was 100.25% ( RSD = 2.24%, n = 6).


CONCLUSIONS
The extraction process is scientific. The method is available with a good reproducibility and can control the quality of Ephedra sinica Dispensing Granules.
Zhou
Bin
B
School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China. super_zhou@eyou.com
Gao
Wen-Yuan
WY
Zhang
Tie-Jun
TJ
Zhang
Jia-Xin
JX
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000592:standards*; D029790:Ephedra sinica / Q000737:chemistry*; D004809:Ephedrine / Q000032:analysis* / Q000302:isolation & purification; D006358:Hot Temperature; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D013678:Technology, Pharmaceutical / Q000379:methods; D013995:Time; D014867:Water / Q000008:administration & dosage
D004365:Drugs, Chinese Herbal; D014867:Water; D004809:Ephedrine