Photografted poly(ethylene glycol) matrix for affinity interaction studies.
A poly(ethylene glycol) (PEG)-based matrix for studies of affinity interactions is developed and demonstrated. The PEG matrix, less than 0.1 microm thick, is graft copolymerized onto a cycloolefin polymer from a mixture of PEG methacrylates using a free radical reaction initiated by UV light at 254 nm. The grafting process is monitored in real time, and characteristics such as thickness, homogeneity, relative composition, photostability, and performance in terms of protein resistance in complex biofluids and sensor qualities are investigated with null ellipsometry, infrared spectroscopy, and surface plasmon resonance. The matrix is subsequently modified to contain carboxyl groups, thereby making it possible to immobilize ligands in a controlled and functional manner. Human serum albumin and fibrinogen are immobilized and successfully detected by antibody recognition using surface plasmon resonance. The results are encouraging and suggest that the PEG matrix is suitable for biochip and biosensor applications in demanding biofluids.
Larsson
Andréas
A
Division of Sensor Science and Molecular Physics, Department of Physics, Chemistry and Biology, Linköping University, SE-581 83 Linköping, Sweden.
Ekblad
Tobias
T
Andersson
Olof
O
Liedberg
Bo
B
D000327:Adsorption; D015374:Biosensing Techniques; D001709:Biotechnology / Q000379:methods*; D005340:Fibrinogen / Q000737:chemistry; D006801:Humans; D008024:Ligands; D008689:Methacrylates / Q000737:chemistry; D046228:Microarray Analysis; D008956:Models, Chemical; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D020349:Surface Plasmon Resonance
D008024:Ligands; D008689:Methacrylates; D011108:Polymers; D012709:Serum Albumin; D011092:Polyethylene Glycols; D005340:Fibrinogen