Engineering bacterial vectors for delivery of genes and proteins to antigen-presenting cells.
Bacterial vectors offer a biological route to gene and protein delivery with this article featuring delivery to antigen-presenting cells (APCs). Primarily in the context of immune stimulation against infectious disease or cancer, the goal of bacterially mediated delivery is to overcome the hurdles to effective macromolecule delivery. This review will present several bacterial vectors as macromolecule (protein or gene) delivery devices with both innate and acquirable (or engineered) biological features to facilitate delivery to APCs. The review will also present topics related to large-scale manufacture, storage, and distribution that must be considered if the bacterial delivery devices are ever to be used in a global market.
Parsa
Saba
S
Department of Chemical and Biological Engineering, Tufts University, Medford, Massachusetts 02155, USA.
Pfeifer
Blaine
B
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D004269:DNA, Bacterial / Q000378:metabolism*; D018014:Gene Transfer Techniques; D005818:Genetic Engineering; D005822:Genetic Vectors; D006801:Humans
D004269:DNA, Bacterial