Increased dietary sodium alters Fos expression in the lamina terminalis during intravenous angiotensin II infusion.
These studies examined the effects of increased dietary sodium on expression of Fos, the protein product of c-fos, in forebrain structures in the rat following intravenous infusion with angiotensin II (AngII). Animals were provided with either tap water (Tap) or isotonic saline solution (Iso) as their sole drinking fluid for 3-5 weeks prior to testing. Rats were then implanted with catheters in a femoral artery and vein. The following day, the conscious, unrestrained animals received iv infusion of either isotonic saline (Veh), AngII, or phenylephrine (Phen) for 2 h. Blood pressure and heart rate were monitored continuously throughout the procedure. Brains were subsequently processed for evaluation of Fos-like immunoreactivity (Fos-Li IR) in the organum vasculosum of the lamina terminalis (OVLT), the subfornical organ (SFO), and the median preoptic nucleus (MnPO). Fos-Li IR was significantly increased in the SFO and OVLT of animals consuming both Tap and Iso following AngII, but not Phen, compared to Veh infusions. Furthermore, Fos-Li IR in the MnPO was increased following AngII infusion in rats consuming a high sodium diet, but not in animals drinking Tap. These data suggest that increased dietary sodium sensitizes the MnPO neurons to excitatory input from brain areas responding to circulating AngII.
Bealer
Steven L
SL
Department of Pharmacology and Toxicology, College of Pharmacy, 20 South 3000 East, Rm 201, University of Utah, Salt Lake City, UT 84121, USA. steve.bealer@deans.pharm.utah.edu
Metcalf
Cameron S
CS
Heyborne
Ryan
R
D000804:Angiotensin II / Q000008:administration & dosage* / Q000494:pharmacology; D000818:Animals; D007031:Hypothalamus / Q000187:drug effects* / Q000378:metabolism*; D007262:Infusions, Intravenous; D008297:Male; D010656:Phenylephrine / Q000494:pharmacology; D011301:Preoptic Area / Q000187:drug effects / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride / Q000494:pharmacology; D012982:Sodium, Dietary / Q000494:pharmacology*; D013356:Subfornical Organ / Q000187:drug effects / Q000378:metabolism
D016760:Proto-Oncogene Proteins c-fos; D012982:Sodium, Dietary; D000804:Angiotensin II; D010656:Phenylephrine; D012965:Sodium Chloride