title: Expression and localization of surfactant proteins in human nasal epithelium.
abstract: Surfactant proteins (SPs), designated SP-A, SP-B, SP-C, and SP-D, play an important role in surfactant metabolism and host defense mechanisms in the lung. This study investigates expression of the different SP types in human nasal mucosa and cultured normal human nasal epithelial (NHNE) cells and whether the expression of SP mRNA is influenced by the degree of mucociliary differentiation. RT-PCR was performed with mRNA from cultured NHNE cells and nasal mucosa. Immunohistochemical staining for SPs was performed on nasal mucosa specimens. Western blot analysis was performed on cell lysates from cultured NHNE cells. SP-A2, SP-B, and SP-D mRNAs were expressed in normal NHNE cells and human nasal mucosa. SPs were localized in ciliated cells of the surface epithelium and serous acini of the submucosal glands. SP-A, SP-B, and SP-D proteins were expressed in cultured NHNE cells. The degree of mucociliary differentiation influenced expression of the SP gene. We demonstrate that SP-A, SP-B, and SP-D are expressed in human nasal mucosa and cultured NHNE cells. Further study of the functional role of SPs in the upper airway is required.
authors.affiliation: Dept. of Otorhinolaryngology-Head and Neck Sugery, Konkuk University College of Medicine, Seoul.
authors.forename: Jin Kook
authors.initials: JK
authors.lastname: Kim
authors.forename: Sung-Shik
authors.initials: SS
authors.lastname: Kim
authors.forename: Keung Won
authors.initials: KW
authors.lastname: Rha
authors.forename: Chang-Hoon
authors.initials: CH
authors.lastname: Kim
authors.forename: Jae Hoon
authors.initials: JH
authors.lastname: Cho
authors.forename: Chang-Hoon
authors.initials: CH
authors.lastname: Lee
authors.forename: Jeung-Gweon
authors.initials: JG
authors.lastname: Lee
authors.forename: Joo-Heon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D002478:Cells, Cultured; D004847:Epithelial Cells / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D009297:Nasal Mucosa / Q000378:metabolism*; D037662:Pulmonary Surfactant-Associated Protein A / Q000096:biosynthesis*; D037701:Pulmonary Surfactant-Associated Protein B / Q000096:biosynthesis*; D037721:Pulmonary Surfactant-Associated Protein C / Q000096:biosynthesis*; D037663:Pulmonary Surfactant-Associated Protein D / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000378:metabolism; D014420:Turbinates / Q000378:metabolism*
chemical_list: D037662:Pulmonary Surfactant-Associated Protein A; D037701:Pulmonary Surfactant-Associated Protein B; D037721:Pulmonary Surfactant-Associated Protein C; D037663:Pulmonary Surfactant-Associated Protein D; D012333:RNA, Messenger; C489679:SFTPC protein, human