Assessment of chromogen suitability in ELISA for the detection of anaplasmosis and trypanosomosis.
Two different ELISAs were routinely performed in our laboratory to detect bovine trypanosomosis and anaplasmosis. The ELISA test for trypanosomosis involved the adsorption of a soluble fraction of parasites as the antigen; and, the ELISA for anaplasmosis was performed with a purified recombinant protein MSP5r adsorbed to the plate. With the purpose of assessing the merit of ABTS and TMB, we compared the absorbance obtained from positive and negative control sera from both assays. The results obtained, suggest that TMB is more adequate for recombinant antigens and that ABTS is preferred when partially purified antigenic extracts are used in the ELISA test.
Reyna-Bello
Armando
A
Universidad Nacional Experimental Simón Rodriguez-IDECYT, Centro de Estudios Biomédicos y Veterinarios, Laboratorio de Inmunobiología, Caracas, Venezuela. areyna@inmunobiologia.com
Eleizalde
Mariana C
MC
Silva
Adriana M
AM
D000712:Anaplasmosis / Q000175:diagnosis / Q000276:immunology* / Q000469:parasitology; D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis* / Q000276:immunology; D002417:Cattle; D002863:Chromogenic Compounds / Q000032:analysis* / Q000737:chemistry; D003116:Color; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D014345:Trypanosoma / Q000276:immunology*; D014352:Trypanosomiasis / Q000175:diagnosis / Q000276:immunology* / Q000469:parasitology
D000913:Antibodies, Protozoan; D002863:Chromogenic Compounds