Immunohistolocalization of carbonic anhydrase isoenzymes (CA-I, -II and -III ) in canine epididymis.
The immunolocalization of the efferent duct and the epididymis in canine was firstly examined using an the immunohistochemical method with the canine carbonic anhydrase (CA) -I, CA-II and CA-III antisera. The efferent duct was immunonegative for all present canine CA antisera. However, some slender shaped epithelial cells in the head and body segments of the epididymal duct were intensely reacted to the CA-II antiserum. These results suggested that the CA-II might be controlled in the luminal environment in the head and body segments of the canine epididymis by the proton and bicarbonate balance for the maintenance of the spermatozoal stability and movement.
Ichihara
Nobutsune
N
Department of Anatomy, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, Japan.
Ishikawa
Takefumi
T
Kaseda
Misato
M
Nishita
Toshiho
T
Amasaki
Hajime
H
Asari
Masao
M
D000818:Animals; D024401:Carbonic Anhydrase I / Q000302:isolation & purification* / Q000378:metabolism; D024402:Carbonic Anhydrase II / Q000302:isolation & purification* / Q000378:metabolism; D024403:Carbonic Anhydrase III / Q000302:isolation & purification* / Q000378:metabolism; D004285:Dogs; D004822:Epididymis / Q000201:enzymology*; D007150:Immunohistochemistry / Q000662:veterinary*; D008297:Male
D024401:Carbonic Anhydrase I; D024402:Carbonic Anhydrase II; D024403:Carbonic Anhydrase III