A Turnip yellow mosaic virus infection system in Arabidopsis suspension cell culture.
Turnip yellow mosaic virus (TYMV) is a positive-strand RNA virus able to infect Arabidopsis thaliana. To establish a TYMV infection system in Arabidopsis cell culture, TYMV replicons with the capsid protein gene replaced by a reporter gene expressing the Sh ble protein conferring zeocin resistance were used to transfect Arabidopsis cells. Zeocin-resistant Arabidopsis calli were used to generate a suspension cell culture. Detection of viral proteins and RNAs after 18 months in culture demonstrated persistent replication of the replicon. The Arabidopsis cell culture yielded soluble, active replication complexes, providing a useful tool to study host factors involved in TYMV replication.
Camborde
Laurent
L
Laboratoire de Virologie Moléculaire, Institut Jacques Monod, UMR 7592 CNRS, Universités Paris 6, Paris 7, 2 place Jussieu, 75251 Paris Cedex 05, France.
Tournier
Vincent
V
Noizet
Mildred
M
Jupin
Isabelle
I
D017360:Arabidopsis / Q000166:cytology / Q000187:drug effects / Q000821:virology*; D001761:Bleomycin / Q000494:pharmacology; D036022:Capsid Proteins / Q000235:genetics; D018929:Cell Culture Techniques; D002460:Cell Line / Q000821:virology*; D004351:Drug Resistance; D015870:Gene Expression; D017930:Genes, Reporter; D010935:Plant Diseases / Q000821:virology*; D012093:Replicon; D013535:Suspensions; D017806:Tymovirus / Q000235:genetics / Q000502:physiology*; D014779:Virus Replication
D036022:Capsid Proteins; D013535:Suspensions; D001761:Bleomycin; C105427:Zeocin