[Simultaneous determination of three active constituents in cortex moutan by RP-HPLC].
OBJECTIVE
To establish a RP-HPLC method for the simultaneous determination of gallic acid, paeoniflorin and paeonol in Cortex Moutan.


METHODS
The analysis was carried out on a column of YMC-C18 (4. 6 mm x 250 mm, 5 microm), and gradient elution was used for the baseline separation of these three constituents with the different detection wavelength.


RESULTS
The linear ranges for gallic acid, paeoniflorin and paeonol were 0.0130-0.267, 0.0880-1.76 and 0.164-3.28 microg, respectively. Their average recoveries were 104.3%, 101.7% and 99.5%, respectively.


CONCLUSIONS
The method was successfully applied to the determination of gallic acid, paeoniflorin and paeonol in Cortex Moutan from the different drugstores. It was shown in this study that the proposed method could be used to evaluate the quality of the medicinal plants or herbal preparations containing gallic acid, paeoniflorin and paeonol.
Wang
Yu-Wen
YW
Department of Chinese Medicine Science and Engineering, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310027, China.
Yu
Ke
K
Cheng
Yi-Yu
YY
D000098:Acetophenones / Q000032:analysis*; D001565:Benzoates / Q000032:analysis*; D001952:Bridged-Ring Compounds / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005707:Gallic Acid / Q000032:analysis*; D005960:Glucosides / Q000032:analysis*; D039821:Monoterpenes; D029596:Paeonia / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D006027:Glycosides
D000098:Acetophenones; D001565:Benzoates; D001952:Bridged-Ring Compounds; D004365:Drugs, Chinese Herbal; D005707:Gallic Acid; D005960:Glucosides; D039821:Monoterpenes; C038881:moutan cortex; C015423:peoniflorin; D006027:Glycosides; C013638:paeonol