title: Elevated cerebrospinal fluid F2-isoprostane levels indicating oxidative stress in healthy siblings of multiple sclerosis patients.
abstract: Lipid peroxidation has been implicated in the pathogenesis of multiple sclerosis (MS). Isoprostanes, isomers of prostaglandins, are produced by free radical-mediated peroxidation of fatty acids in vivo and can be quantified in biological fluids. This study examines the levels of cerebrospinal fluid (CSF) F2-isoprostanes (F2-iPs) in MS patients (n=46), their healthy siblings (n=46) and unrelated controls (n=50). The median CSF F2-iP concentration (range) was significantly higher in siblings of MS patients, as compared to healthy controls (40.0 [7.1-68.7] and 29.1 [6.4-60.3] pg/mL, respectively, p=0.031). MS patients demonstrated F2-iP levels intermediate between siblings and controls. F2-iP levels in MS patients and siblings correlated significantly (R=0.360, p=0.012). These results suggest that siblings of MS patients have an increased oxidative stress response to environmental and/or genetic factors that may be involved in MS pathogenesis.
authors.affiliation: Clinical Neurochemistry Laboratory, Department of Neuroscience and Physiology, Sahlgren's University Hospital, S-431 80 Mölndal, Sweden.
authors.forename: Niklas
authors.initials: N
authors.lastname: Mattsson
authors.forename: Sara
authors.initials: S
authors.lastname: Haghighi
authors.forename: Oluf
authors.initials: O
authors.lastname: Andersen
authors.forename: Yuemang
authors.initials: Y
authors.lastname: Yao
authors.forename: Lars
authors.initials: L
authors.lastname: Rosengren
authors.forename: Kaj
authors.initials: K
authors.lastname: Blennow
authors.forename: Domenico
authors.initials: D
authors.lastname: Praticò
authors.forename: Henrik
authors.initials: H
authors.lastname: Zetterberg
mesh_terms: D000328:Adult; D000368:Aged; D002490:Central Nervous System / Q000378:metabolism / Q000503:physiopathology; D004781:Environmental Exposure; D028441:F2-Isoprostanes / Q000032:analysis / Q000134:cerebrospinal fluid*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid* / Q000235:genetics / Q000503:physiopathology; D018384:Oxidative Stress / Q000235:genetics*; D035781:Siblings; D015854:Up-Regulation / Q000235:genetics*
chemical_list: D028441:F2-Isoprostanes