Ganoderma lucidum polysaccharide peptide reduced the production of proinflammatory cytokines in activated rheumatoid synovial fibroblast.
The aim of the current study was to elucidate the potential therapeutic effect of Ganoderma lucidum polysaccharide peptide (GL-PP) in rheumatoid arthritis (RA). The effects of GL-PP on cell proliferation and cytokine production were studied in RA synovial fibroblasts (RASF). GL-PP significantly inhibited the proliferation of RASF. Following the incubation with GL-PP, production of interleukin (IL)-6 and monocyte chemoattractant protein (MCP)-1 in RASF were significantly increased as expressed as percentage change from basal values. However, the actual effects were minimal due to the low basal values. When RASF were activated by IL-1beta or lipopolysaccharides, IL-8 and MCP-1 production increased many folds. GL-PP significantly suppressed their productions. The inhibitory effects of GL-PP on cytokine production in RASF were at least in part, by inhibiting the nuclear factor-kappa B (NF-kappaB) transcription pathway. Our results demonstrated that GL-PP had the unique ability to modulate cytokine production in RASF and warrants further investigation into its mechanism of action.
Ho
Y W
YW
Department of Pharmacology, University of Hong Kong, Hong Kong SAR, Hong Kong.
Yeung
J S L
JS
Chiu
P K Y
PK
Tang
W M
WM
Lin
Z B
ZB
Man
R Y K
RY
Lau
C S
CS
D001172:Arthritis, Rheumatoid / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008516:Medicine, Chinese Traditional; D016328:NF-kappa B / Q000378:metabolism; D011509:Proteoglycans / Q000494:pharmacology / Q000627:therapeutic use; D020940:Reishi / Q000737:chemistry*; D013583:Synovial Membrane / Q000166:cytology*
D016207:Cytokines; D016328:NF-kappa B; D011509:Proteoglycans; C093216:polysaccharide peptide