title: A combined dataset of human cerebrospinal fluid proteins identified by multi-dimensional chromatography and tandem mass spectrometry.
abstract: Human cerebrospinal fluid (CSF) is an important source for studying protein biomarkers of age-related neurodegenerative diseases. Before characterizing biomarkers unique to each disease, it is necessary to categorize CSF proteins systematically and extensively. However, the enormous complexity, great dynamic range of protein concentrations, and tremendous protein heterogeneity due to post-translational modification of CSF create significant challenges to the existing proteomics technologies for an in-depth, nonbiased profiling of the human CSF proteome. To circumvent these difficulties, in the last few years, we have utilized several different separation methodologies and mass spectrometric platforms that greatly enhanced the identification coverage and the depth of protein profiling of CSF to characterize CSF proteome. In total, 2594 proteins were identified in well-characterized pooled human CSF samples using stringent proteomics criteria. This report summarizes our efforts to comprehensively characterize the human CSF proteome to date.
authors.affiliation: Department of Pathology, University of Washington, Seattle, WA 98104, USA.
authors.forename: Sheng
authors.initials: S
authors.lastname: Pan
authors.forename: David
authors.initials: D
authors.lastname: Zhu
authors.forename: Joseph F
authors.initials: JF
authors.lastname: Quinn
authors.forename: Elaine R
authors.initials: ER
authors.lastname: Peskind
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Montine
authors.forename: Biaoyang
authors.initials: B
authors.lastname: Lin
authors.forename: David R
authors.initials: DR
authors.lastname: Goodlett
authors.forename: Greg
authors.initials: G
authors.lastname: Taylor
authors.forename: Jimmy
authors.initials: J
authors.lastname: Eng
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
mesh_terms: D002556:Cerebrospinal Fluid Proteins / Q000032:analysis* / Q000737:chemistry*; D002853:Chromatography, Liquid; D006801:Humans; D020543:Proteome; D053719:Tandem Mass Spectrometry
chemical_list: D002556:Cerebrospinal Fluid Proteins; D020543:Proteome