Construction of a dimethyl sulfoxide sensor based on dimethyl sulfoxide reductase immobilized on a au film electrode.
A novel dimethyl sulfoxide (DMSO) sensor using DMSO reductase and film electrodes was constructed. The Au and Ag electrodes were fabricated on slide glass by vacuum deposition and the application of a photolithographic technique. The micro-chamber (4 x 50 x 1 mm, volume 200 microl) was fabricated on a poly(dimethylsiloxane) (PDMS) polymer. The Pt electrode was implanted in a PDMS polymer. DMSO reductase was immobilized on a Au film electrode with bovine serum albumin (BSA)-glutaraldehyde. This sensor could determine DMSO in an unpurged aqueous solution with glucose oxidase (GOD) and catalase (CAT) for oxygen removal. The DMSO sensor showed a linear response within 1 mM DMSO with a correlation coefficient of 0.999. The detection limit was 200 microM (3sigma), and the sensitivity was 23.8 mA M(-1) cm(-2). The relative standard deviations at each concentration were within 3.6%.
Yonehara
Hiroki
H
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
Fujii
Shin-Ichiro
S
Sato
Kiichi
K
Abo
Mitsuru
M
Yoshimura
Etsuro
E
D002138:Calibration; D004121:Dimethyl Sulfoxide / Q000032:analysis*; D004563:Electrochemistry; D004566:Electrodes; D004784:Environmental Monitoring; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005504:Food Analysis; D007506:Iron-Sulfur Proteins / Q000378:metabolism*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D010100:Oxygen / Q000737:chemistry; D011786:Quality Control; D012242:Rhodobacter sphaeroides / Q000201:enzymology
D004800:Enzymes, Immobilized; D007506:Iron-Sulfur Proteins; D010088:Oxidoreductases; C045664:dimethyl sulfoxide reductase; D010100:Oxygen; D004121:Dimethyl Sulfoxide