The alpha(1S) subunit of the L-type calcium channel is not a predisposition gene for thyrotoxic periodic paralysis.
OBJECTIVE
Thyrotoxic periodic paralysis (TTP) has been associated with genetic variations in the gene encoding the alpha 1 subunit of the L-type calcium channel (CACNA1S). Mutations in CACNA1S are known to account for the majority of cases of familial hypokalaemic periodic paralysis (HOKPP). In this study we have examined 48 genetic polymorphisms in the CACNA1S gene and genotyped a tagging set of representative polymorphisms to determine the role of this gene in TPP.


METHODS
A genetic association study was carried out with 98 TPP patients and 162 male thyrotoxic controls. Among 47 polymorphisms evaluated for linkage disequilibrium (LD) and the spectrum of haplotypes in the Chinese population, 31 were selected as tagging single-nucleotide polymorphisms (SNPs) for genotyping the whole sample. A new genotyping protocol was used to analyse an insertion/deletion (I/D) polymorphism.


RESULTS
We studied the LD among 47 polymorphisms in the CACNA1S gene, which comprised a set of high-density markers with an average of one SNP every 2 kb. Subsequently, 31 tagSNPs were genotyped for all the samples. The gene is composed of three LD blocks. With this block structure, we were confident that variations of the gene were comprehensively covered by the tagSNPs. No significant association was found between the polymorphisms and TPP.


CONCLUSIONS
We established the LD structure of this calcium channel subunit gene (CACNA1S) for the first time. However, its genetic variations are not associated with TPP in Chinese patients.
Tang
Nelson L S
NL
Department of Chemical Pathology, Faculty of Medicine, The Chinese Univerisity of Hong Kong SAR, China. nelsontang@cuhk.edu.hk
Chow
C C
CC
Ko
Gary T C
GT
Tai
Morris H L
MH
Kwok
Rachel
R
Yao
X Q
XQ
Cockram
Clive S
CS
D000328:Adult; D044466:Asian People; D015220:Calcium Channels / Q000235:genetics*; D020746:Calcium Channels, L-Type; D016022:Case-Control Studies; D004252:DNA Mutational Analysis; D020022:Genetic Predisposition to Disease; D006801:Humans; D020514:Hypokalemic Periodic Paralysis / Q000208:ethnology / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide
C412223:CACNA1S protein, human; D015220:Calcium Channels; D020746:Calcium Channels, L-Type