title: Comparison of core needle biopsy and fine-needle aspiration of enlarged peripheral lymph nodes for antemortem diagnosis of enzootic bovine lymphosarcoma in cattle.
abstract: OBJECTIVE
  To determine whether antemortem core needle biopsy and fine-needle aspiration of enlarged peripheral lymph nodes could be used to distinguish between inflammation and lymphosarcoma in cattle.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  25 cattle with enlarged peripheral lymph nodes.
  
  
  METHODS
  Antemortem biopsies of the selected lymph nodes were performed with an 18-gauge, 12-cm core needle biopsy instrument. Fine-needle aspirates were performed with a 20-gauge, 4-cm needle. Specimens were analyzed by pathologists who were unaware of clinical findings and final necropsy findings, and specimens were categorized as reactive, neoplastic, or nondiagnostic for comparison with necropsy results.
  
  
  RESULTS
  Sensitivity and specificity of core needle biopsy ranged from 38% to 67% and from 80% to 25%, respectively. Sensitivity of fine-needle aspiration ranged from 41% to 53%, and specificity was 100%. Predictive values for positive test results ranged from 77% to 89% for core needle biopsy and were 100% for fine-needle aspiration. Predictive values for negative test results were low for both core needle biopsy and fine-needle aspiration.
  
  
  CONCLUSIONS
  Results indicated that core needle biopsy and fine-needle aspiration can aid in the antemortem diagnosis of bovine enzootic lymphosarcoma. Results of fine-needle aspiration of enlarged peripheral lymph nodes were more specific and more predictive for a positive test result than were results of core needle biopsy.
authors.affiliation: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK 74078, USA.
authors.forename: Kevin E
authors.initials: KE
authors.lastname: Washburn
authors.forename: Robert N
authors.initials: RN
authors.lastname: Streeter
authors.forename: Terry W
authors.initials: TW
authors.lastname: Lehenbauer
authors.forename: Timothy A
authors.initials: TA
authors.lastname: Snider
authors.forename: Grant B
authors.initials: GB
authors.lastname: Rezabek
authors.forename: Jerry W
authors.initials: JW
authors.lastname: Ritchey
authors.forename: James H
authors.initials: JH
authors.lastname: Meinkoth
authors.forename: Robin W
authors.initials: RW
authors.lastname: Allison
authors.forename: Theresa E
authors.initials: TE
authors.lastname: Rizzi
authors.forename: Melanie J
authors.initials: MJ
authors.lastname: Boileau
mesh_terms: D000818:Animals; D044963:Biopsy, Fine-Needle / Q000379:methods / Q000662:veterinary*; D001707:Biopsy, Needle / Q000379:methods / Q000662:veterinary*; D002417:Cattle; D003937:Diagnosis, Differential; D016583:Enzootic Bovine Leukosis / Q000175:diagnosis / Q000473:pathology*; D008198:Lymph Nodes / Q000473:pathology; D011237:Predictive Value of Tests; D011446:Prospective Studies; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity