Formulation and cytotoxicity of doxorubicin loaded in self-assembled bio-polyelectrolyte microshells.
A bio-polyelectrolyte microshell composed of alginate sodium (ALG) and chitosan (CHI) was fabricated by electrostatic layer-by-layer (LbL) self-assembly technique. The resulting ALG-CHI microshells were found to be able to effectively load anti-cancer drug doxorubicin (DOX) in the interior of the shells under modest conditions without addition of other reagents, as demonstrated by confocal laser scanning microscopy (CLSM). The mass of DOX loaded in one capsule of four alginate/chitosan layers (i.e. the volume V=2.5x10(-10) cm3) is calculated as ca. 1.4x10(-13) g, which corresponds to 1.5x10(8) DOX molecules. Also, the release of DOX in the shells is dependent on the number of assembled layers of the shells. Colorimetric XTT cell viability assay results showed that the DOX-loaded microshells at high concentrations tested could kill cancer cells more efficiently than free-DOX alone.
Tao
Xia
X
Key Lab for Nanomaterials of the Ministry of Education, Beijing University of Chemical Technology, Beijing 100029, China. taoxia@yahoo.com
Chen
Hua
H
Sun
Xue-Jun
XJ
Chen
Jian-Feng
JF
Roa
Wilson H
WH
D000464:Alginates / Q000737:chemistry; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry; D003124:Colorimetry; D003692:Delayed-Action Preparations; D004317:Doxorubicin / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry; D004573:Electrolytes; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D018613:Microscopy, Confocal; D008863:Microspheres; D055672:Static Electricity; D013678:Technology, Pharmaceutical
D000464:Alginates; D000903:Antibiotics, Antineoplastic; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D004573:Electrolytes; D006603:Hexuronic Acids; D004317:Doxorubicin; D020723:Glucuronic Acid; D048271:Chitosan