High-level expression of human lactoferrin in the milk of goats by using replication-defective adenoviral vectors.
The expression of human lactoferrin in the mammary gland is an attractive approach to diminish its current production cost. Previous attempts to produce lactorferrin in the milk of transgenic animals resulted in very high cost and uncertain results. In this paper, we have directly infused replication-defective adenovirus encoding human lactoferrin cDNA into the mammary gland of goats via the teat canal. In this way, we obtained a high level of expressed human lactoferrin up to 2g/L in the milk of goats. The milk serum was collected from the infected mammary gland 48 h post-infection and subjected to a 10% SDS-PAGE and Western blotting. A approximately 80-kDa protein was visualized after viral vector infection. Our results demonstrate that intraductal injection of recombinant replication-defective adenovirus vectors may provide a very useful tool for large-scale production of recombinant proteins of biopharmaceutical interest.
Han
Zeng-Sheng
ZS
College of Animal Science and Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province 712100, PR China.
Li
Qing-Wang
QW
Zhang
Zhi-Ying
ZY
Xiao
Bo
B
Gao
Da-Wei
DW
Wu
Shu-Yun
SY
Li
Jian
J
Zhao
Hong-Wei
HW
Jiang
Zhong-Liang
ZL
Hu
Jian-Hong
JH
D000256:Adenoviridae / Q000235:genetics* / Q000502:physiology; D000818:Animals; D015153:Blotting, Western; D018929:Cell Culture Techniques; D002460:Cell Line; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D017364:Collagenases / Q000494:pharmacology; D004261:DNA Replication; D018076:DNA, Complementary; D004279:DNA, Viral / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D005822:Genetic Vectors; D006041:Goats; D006801:Humans; D007781:Lactoferrin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000033:anatomy & histology / Q000166:cytology / Q000378:metabolism; D008892:Milk / Q000821:virology*; D008970:Molecular Weight; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D014162:Transfection
D018076:DNA, Complementary; D004279:DNA, Viral; D011994:Recombinant Proteins; D007781:Lactoferrin; D017364:Collagenases