Identification of New World Leishmania species from Peru by biochemical techniques and multiplex PCR assay.
We have characterized diverse strains or species of Leishmania isolated in humans that are currently circulating throughout Peru, by means of isoenzymatic characterization, kDNA analysis by restriction enzymes, and multiplex PCR assay. The cluster analysis gave five groups. Cluster 1 includes L. (L.) donovani together with the isolates LP4 and LP7, forming the donovani complex. Thus, this complex corresponds to the New World visceral form, L. (L.) chagasi. Cluster 2 is formed by the isolates LP1-LP3, LP6, LP10, LP9, and LP11, phylogenetically intermediate between Cluster 1 and Cluster 3, or they can be treated as hybrids. Cluster 3 is divided into two subgroups: one formed by L. (V.) peruviana, together with the isolates LP14 and LP5, and the second one formed by L. (V.) brazilensis and the isolate LP8. These two subgroups form part of the brazilensis complex. The three strains of L. (L.) infantum [L. (L.) infantum I and II and la LSI] make up Cluster 4. In Cluster 5, we include the three Mexican strains (LM1-LM3) forming one subgroup while we would place L. (L.) amazonensis in another subgroup. These two subgroups would comprise the complex mexicana.
Rodríguez-González
Isabel
I
Instituto de Biotecnología, Departamento de Parasitología, Facultad de Ciencias, Universidad de Granada, Granada, Spain.
Marín
Clotilde
C
Longoni
Silvia S
SS
Mateo
Hector
H
Alunda
José M
JM
Minaya
Gloria
G
Gutiérrez-Sánchez
Ramón
R
Vargas
Franklin
F
Sánchez-Moreno
Manuel
M
D000818:Animals; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004262:DNA Restriction Enzymes / Q000378:metabolism; D018105:DNA, Kinetoplast / Q000235:genetics / Q000378:metabolism*; D007525:Isoelectric Focusing; D007527:Isoenzymes; D007891:Leishmania / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D007896:Leishmaniasis / Q000469:parasitology*; D010568:Peru; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D015800:Protozoan Proteins / Q000032:analysis / Q000302:isolation & purification
D018105:DNA, Kinetoplast; D007527:Isoenzymes; D015800:Protozoan Proteins; D004262:DNA Restriction Enzymes