title: Intersectin is a negative regulator of dynamin recruitment to the synaptic endocytic zone in the central synapse.
abstract: Intersectin is a multidomain dynamin-binding protein implicated in numerous functions in the nervous system, including synapse formation and endocytosis. Here, we demonstrate that during neurotransmitter release in the central synapse, intersectin, like its binding partner dynamin, is redistributed from the synaptic vesicle pool to the periactive zone. Acute perturbation of the intersectin-dynamin interaction by microinjection of either intersectin antibodies or Src homology 3 (SH3) domains inhibited endocytosis at the fission step. Although the morphological effects induced by the different reagents were similar, antibody injections resulted in a dramatic increase in dynamin immunoreactivity around coated pits and at constricted necks, whereas synapses microinjected with the GST (glutathione S-transferase)-SH3C domain displayed reduced amounts of dynamin in the neck region. Our data suggest that intersectin controls the amount of dynamin released from the synaptic vesicle cluster to the periactive zone and that it may regulate fission of clathrin-coated intermediates.
authors.affiliation: Department of Neuroscience, Karolinska Institutet, 171 77 Stockholm, Sweden.
authors.forename: Emma
authors.initials: E
authors.lastname: Evergren
authors.forename: Helge
authors.initials: H
authors.lastname: Gad
authors.forename: Kristin
authors.initials: K
authors.lastname: Walther
authors.forename: Anna
authors.initials: A
authors.lastname: Sundborger
authors.forename: Nikolay
authors.initials: N
authors.lastname: Tomilin
authors.forename: Oleg
authors.initials: O
authors.lastname: Shupliakov
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000502:physiology*; D000595:Amino Acid Sequence; D000818:Animals; D034281:Dynamins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004705:Endocytosis / Q000502:physiology*; D011992:Endosomes / Q000502:physiology / Q000648:ultrastructure; D007798:Lampreys; D008969:Molecular Sequence Data; D009433:Neural Inhibition / Q000502:physiology; D013569:Synapses / Q000502:physiology* / Q000648:ultrastructure; D013572:Synaptic Vesicles / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; C115919:intersectin 1; D034281:Dynamins