title: PKC-beta II expression has prognostic impact in nodal diffuse large B-cell lymphoma.
abstract: Recent studies of gene expression and immunohistochemistry have shown that protein kinase C-beta II (PKC-beta II) might have prognostic significance in patients with diffuse large B-cell lymphoma (DLBCL). We sought to determine the prognostic significance of the expression of PKC-beta II in patients with nodal DLBCL. Formalin-fixed, paraffin-embedded tissues were stained with a monoclonal antibody to PKC-beta II protein. A total of 125 patients were studied; 83 patients (66%) were in the low-risk International Prognostic Index (IPI) group. Forty-eight patients (38%) were positive for PKC-beta II. Complete remission was obtained in 70%, and was not influenced by the PKC-beta II status (67 vs 71%). The 5-year event-free survival (EFS) was worse in high-risk patients (14 vs 58%, P<0.001) and in those with PKC-beta II positivity (36 vs 49%, P=0.054). In low-risk IPI patients, PKC-beta II expression was related to a worse 5-year overall survival (OS) (60 vs 76%, P=0.033) and a worse 5-year EFS (48 vs 66%, P=0.014). In a Cox regression analysis for EFS, both PKC-beta II expression (hazard ratio=1.68, P=0.037) and the IPI (HR=3.07, P<0.001) were independent poor prognostic factors. PKC-beta II (HR=1.72, P=0.046) and the IPI (HR=5.16, P<0.001) were also independent poor prognostic factors for the OS. PKC-beta II expression, along with the IPI, were associated with a worse EFS and OS in patients with nodal DLBCL specially in low-risk IPI patients.
authors.affiliation: Department of Internal Medicine/Hematology, University Hospital, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil. rony@hucff.ufrj.br
authors.forename: Rony
authors.initials: R
authors.lastname: Schaffel
authors.forename: José C
authors.initials: JC
authors.lastname: Morais
authors.forename: Irene
authors.initials: I
authors.lastname: Biasoli
authors.forename: Joana
authors.initials: J
authors.lastname: Lima
authors.forename: Adriana
authors.initials: A
authors.lastname: Scheliga
authors.forename: Sergio
authors.initials: S
authors.lastname: Romano
authors.forename: Cristiane
authors.initials: C
authors.lastname: Milito
authors.forename: Nelson
authors.initials: N
authors.lastname: Spector
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016393:Lymphoma, B-Cell / Q000201:enzymology* / Q000401:mortality; D016403:Lymphoma, Large B-Cell, Diffuse / Q000201:enzymology* / Q000401:mortality; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D011493:Protein Kinase C / Q000096:biosynthesis*; D064546:Protein Kinase C beta; D016019:Survival Analysis
chemical_list: D014408:Biomarkers, Tumor; D011493:Protein Kinase C; D064546:Protein Kinase C beta