title: Tissue-specific expression and molecular modeling of cytosolic manganese superoxide dismutases from the white shrimp Litopenaeus vannamei.
abstract: Manganese superoxide dismutases (MnSODs) are usually mitochondrial enzymes, although there are few examples of cytosolic MnSODs (cMnSOD). We have previously characterized a cMnSOD cDNA from Litopenaeus vannamei hemocytes, and to obtain new insights into the tissue specific expression and the protein structure, we characterized three more different cMnSOD transcripts (cMnsod1, cMnsod2 and cMnsod3) and modeled the three-dimensional protein structure using human MnSOD as a template. The nucleotide sequences differ in seven positions. Four differences are silent; while three produce changes in amino acid sequence. cMnsod1, cMnsod2 and cMnsod3 are differentially expressed in nervous system, hepatopancreas and hemocytes. The structural protein model predicts bona fide MnSODs with proper coordination for the enzymatic activity.
authors.affiliation: Aquatic Molecular Biology Laboratory, Centro de Investigación en Alimentación y Desarrollo, P.O. Box 1735; Hermosillo Son; 83000, México.
authors.forename: Gracia A
authors.initials: GA
authors.lastname: Gómez-Anduro
authors.forename: Rogerio R
authors.initials: RR
authors.lastname: Sotelo-Mundo
authors.forename: Adriana
authors.initials: A
authors.lastname: Muhlia-Almazán
authors.forename: Gloria
authors.initials: G
authors.lastname: Yepiz-Plascencia
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003600:Cytosol / Q000201:enzymology; D020224:Expressed Sequence Tags; D015870:Gene Expression; D008958:Models, Molecular; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000201:enzymology* / Q000235:genetics; D011487:Protein Conformation; D017385:Sequence Homology; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000235:genetics*; D014018:Tissue Distribution
chemical_list: D013482:Superoxide Dismutase