title: GSTT1 and GSTM1 polymorphism in cigarette smokers with head and neck squamous cell carcinoma.
abstract: Gene variability related to carcinogen activation and detoxification may interfere with susceptibility to head and neck cancer.
  
  
  OBJECTIVE
  To investigate the relation between GSTT1 and GSTM1 null polymorphisms and the risk of head and neck squamous cell carcinoma in cigarette smokers.
  
  
  METHODS
  A case-control study conducted at the Sao Jose do Rio Preto Medical School, Brazil. GSTM1 and GSTT1 null genotype frequencies were evaluated by multiplex PCR in 45 cigarette smokers with head and neck squamous cell carcinomas and 45 cigarette smokers without this disease.
  
  
  RESULTS
  The oral cavity was the most prevalent tumor site for squamous cell carcinoma. The GSTT1 null genotype was found in 33.3% of the Experimental Group and 23.3% of the Control Group (p= 0.311). Experimental and Control Groups had GSTM1 null genotype frequencies of 35% and 48.3% (p=0.582). No association between alcohol consumption and GSTT1 and GSTMI null genotypes was found in these groups (p-values>0.05). There were more men, and alcohol consumption was prevalent in both groups.
  
  
  CONCLUSIONS
  In this study we were unable to show a correlation between GSTM1 and GSTT1 genotypes and the development of head and neck squamous cell carcinomas in cigarette smokers.
authors.affiliation: Faculdade de Medicina de São José do Rio Preto, FAMERP, Molecular Biology Department, SP, Brazil.
authors.forename: Joice Matos
authors.initials: JM
authors.lastname: Biselli
authors.forename: Renata Cristina
authors.initials: RC
authors.lastname: de Angelo Calsaverini Leal
authors.forename: Mariângela Torreglosa
authors.initials: MT
authors.lastname: Ruiz
authors.forename: Eny Maria
authors.initials: EM
authors.lastname: Goloni-Bertollo
authors.forename: José Victor
authors.initials: JV
authors.lastname: Maníglia
authors.forename: Andréa Regina Baptista
authors.initials: AR
authors.lastname: Rossit
authors.forename: Erika Cristina
authors.initials: EC
authors.lastname: Pavarino-Bertelli
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000209:etiology / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006258:Head and Neck Neoplasms / Q000201:enzymology / Q000209:etiology / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects / Q000235:genetics*
chemical_list: D005982:Glutathione Transferase; C413545:glutathione S-transferase T1