title: Herpes simplex virus type 1 tegument proteins VP1/2 and UL37 are associated with intranuclear capsids.
abstract: The assembly of the tegument of herpes simplex virus type 1 (HSV-1) is a complex process that involves a number of events at various sites within virus-infected cells. Our studies focused on determining whether tegument proteins, VP1/2 and UL37, are added to capsids located within the nucleus. Capsids were isolated from the nuclear fraction of HSV-1-infected cells and purified by rate-zonal centrifugation to separate B capsids (containing the scaffold proteins and no viral DNA) and C capsids (containing DNA and no scaffold proteins). Western blot analyses of these capsids indicated that VP1/2 associated primarily with C capsids and UL37 associated with B and C capsids. The results demonstrate that at least two of the tegument proteins of HSV-1 are associated with capsids isolated from the nuclear fraction, and these capsid-tegument protein interactions may represent initial events of the tegumentation process.
authors.affiliation: Department of Microbiology and Immunology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA.
authors.forename: Michelle A
authors.initials: MA
authors.lastname: Bucks
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: O'Regan
authors.forename: Michael A
authors.initials: MA
authors.lastname: Murphy
authors.forename: John W
authors.initials: JW
authors.lastname: Wills
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Courtney
mesh_terms: D000818:Animals; D002213:Capsid / Q000737:chemistry / Q000502:physiology; D002467:Cell Nucleus / Q000378:metabolism; D002522:Chlorocebus aethiops; D018259:Herpesvirus 1, Human / Q000737:chemistry / Q000502:physiology*; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000378:metabolism*; D015678:Viral Structural Proteins / Q000276:immunology*; D019065:Virus Assembly
chemical_list: C084763:UL37 protein, Human herpesvirus 1; D014759:Viral Envelope Proteins; D015678:Viral Structural Proteins