title: Functional proteome analysis of the banana plant (Musa spp.) using de novo sequence analysis of derivatized peptides.
abstract: We report the use of chemical derivatization with MALDI-MS/MS analysis for de novo sequence analysis. Using three frequently used homology-based search algorithms, we were able to identify more than 40 proteins from banana, a nonmodel plant with unsequenced genome. Furthermore, this approach allowed the identification of different isoforms. We also observed that the identification score obtained varied according to the position of the peptide sequences in the query using the MS-Blast algorithm.
authors.affiliation: Department of Biochemistry, Physiology and Microbiology, Laboratory of Protein Biochemistry and Protein Engineering, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium. bart.samyn@UGent.be
authors.forename: Bart
authors.initials: B
authors.lastname: Samyn
authors.forename: Kjell
authors.initials: K
authors.lastname: Sergeant
authors.forename: Sebastien
authors.initials: S
authors.lastname: Carpentier
authors.forename: Griet
authors.initials: G
authors.lastname: Debyser
authors.forename: Bart
authors.initials: B
authors.lastname: Panis
authors.forename: Rony
authors.initials: R
authors.lastname: Swennen
authors.forename: Jozef
authors.initials: J
authors.lastname: Van Beeumen
mesh_terms: D000465:Algorithms; D000595:Amino Acid Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D018745:Genome, Plant; D005838:Genotype; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D028521:Musa / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D020539:Sequence Analysis, Protein; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014357:Trypsin / Q000494:pharmacology
chemical_list: D010455:Peptides; D010940:Plant Proteins; D020543:Proteome; D014357:Trypsin