Protective effect of a novel rice extract against ethanol-induced gastric mucosal injury in rat.
The aim of this study was to investigate the protective action of rice extract on ethanol-induced mucosal damage in vivo and wound healing of epithelial cells in vitro. Also, the effect of rice extract on gastric mucosal prostaglandin E(2) level, HSP72 expression, gastric acid secretion, and contribution of vanilloid receptor-mediated action was studied. In addition, using cultured gastric mucosal cells (RGM-1), the effect of rice extract on cytoprotection and wound healing of epithelial cells was evaluated. Rice extract significantly reduced gastric mucosal damage produced by ethanol in vivo, and heat treatment (80 degrees C, 3 min) of this agent did not alter its protective effect. Rice extract also protected RGM-1 from ethanol-induced damage in a dose-dependent manner. Rice extract accelerated wound healing of gastric epithelial cells. Our results demonstrate that rice extract could be an alternative ulcer treatment that provides cytoprotection and enhancement of wound healing not dependent on acid secretion, prostaglandin E(2) level, HSP72 expression, or vanilloid receptors.
Matsuhashi
Tamotsu
T
First Department of Internal Medicine, Akita University School of Medicine, 1-1-1 Hondo, Akita City, Akita, Japan.
Otaka
Michiro
M
Odashima
Masaru
M
Jin
Mario
M
Komatsu
Koga
K
Wada
Isao
I
Horikawa
Youhei
Y
Ohba
Reina
R
Oyake
Jinko
J
Hatakeyama
Natsumi
N
Watanabe
Sumio
S
D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D019610:Cytoprotection / Q000187:drug effects*; D015232:Dinoprostone / Q000378:metabolism; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects; D000431:Ethanol / Q000633:toxicity; D005744:Gastric Acid / Q000378:metabolism; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D050884:HSP72 Heat-Shock Proteins / Q000378:metabolism; D008297:Male; D012275:Oryza; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013276:Stomach Ulcer / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*; D050916:TRPV Cation Channels / Q000378:metabolism; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects
D000897:Anti-Ulcer Agents; D050884:HSP72 Heat-Shock Proteins; D010936:Plant Extracts; D050916:TRPV Cation Channels; C493922:Trpv1 protein, rat; D000431:Ethanol; D015232:Dinoprostone