Phenotypically plastic responses of green frog embryos to conflicting predation risk.
Predators have been shown to alter the timing of switch points between life history stages, but few studies have addressed switch point plasticity in prey exposed simultaneously to conflicting predation pressure. We tested hatching responses of green frog (Rana clamitans) embryos subject to perceived predation risk from chemical cues released by two stage-specific predators, predicting that these predators would elicit: (1) directional hatching responses when presented independently, and (2) intermediate phenotypic responses when presented simultaneously. R. clamitans embryos in outdoor exclosures were exposed to cues from an egg predator (freshwater leeches; Nephelopsis obscura), a larval predator (dragonfly nymphs, Aeschna canadensis), and both predators in a 2 x 2 factorial experiment, and changes in hatchling size, hatchling developmental stage, and hatching time were compared to those for control embryos. Leeches alone induced embryos to hatch at a smaller size and an earlier developmental stage than controls, while dragonfly nymphs elicited a delay in egg hatching time that was associated with larger size and later developmental stage at hatching. Embryos failed to respond to simultaneous exposure to both predators, implying that responses to each occurred concurrently and were therefore dampened. Our results indicate that prey under threat from conflicting predators may manifest intermediate defensive phenotypes. Such intermediate responses may result in elevated rates of prey mortality with possible consequences at the population level.
Ireland
D H
DH
Department of Biology, Trent University, 1600 West Bank Drive, Peterborough, ON K9J 7B8, Canada.
Wirsing
A J
AJ
Murray
D L
DL
D000222:Adaptation, Physiological; D000818:Animals; D003463:Cues; D004625:Embryo, Nonmammalian / Q000502:physiology; D047108:Embryonic Development; D020387:Food Chain; D007313:Insecta / Q000502:physiology; D007865:Leeches / Q000502:physiology; D009758:Nymph / Q000502:physiology; D010641:Phenotype; D011235:Predatory Behavior; D011898:Ranidae / Q000196:embryology* / Q000254:growth & development / Q000502:physiology; D012306:Risk