Gene expression and generation of CD28-CD8 T cells mediated by interleukin 15.
Accumulation of CD28(-)CD8 T cells that are defective in response to antigenic stimulation is a hallmark of age-associated decline in T cell function. However, the underlying mechanism of this age-associated change is not fully understood. We recently analyzed the global gene expression profiles of CD8 T cell subsets from nai ve to memory (CD28(+) to CD28(-)) cells and the growth of CD28(+) and CD28(-)CD8 memory T cells in response to homeostatic cytokine interleukin 15 (IL-15). At the gene expression level, one of the most striking changes is the altered expression of some co-stimulatory receptors and various NK cell receptors in CD28(-)CD8 T cells. Furthermore, CD28(-)CD8 T cells appear to have a normal proliferation response to IL-15 in vitro. Interestingly, IL-15 is also capable of inducing stable loss of CD28 expression in actively dividing CD28(+)CD8 memory T cells. Together, these findings provide the gene expression features of CD28(-)CD8 T cells that differ from their CD28(+) counterparts and suggest a possible role of IL-15 in the increase of CD28(-)CD8 T cells that occurs with aging.
Godlove
Jason
J
Laboratory of Immunology, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA.
Chiu
Wai Kan
WK
Weng
Nan-ping
NP
D015703:Antigens, CD / Q000276:immunology; D018106:CD28 Antigens / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002455:Cell Division; D016922:Cellular Senescence / Q000276:immunology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000276:immunology*; D006801:Humans; D019409:Interleukin-15 / Q000276:immunology* / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*
D015703:Antigens, CD; D018106:CD28 Antigens; D019409:Interleukin-15