title: Capillary electrophoresis of the mycotoxin zearalenone using cyclodextrin-enhanced fluorescence.
abstract: Certain of the cyclodextrins are capable of significantly enhancing the native fluorescence of the estrogenic mycotoxin zearalenone (ZEN). Twenty-two cyclodextrins (CDs) were screened for their ability to enhance the fluorescence of ZEN in a capillary electrophoresis-laser induced fluorescence (CE-LIF) format. Of the CDs that were examined heptakis (2,6-di-O-methyl)-beta-CD gave the greatest enhancement. The heptakis (2,6-di-O-methyl)-beta-CD was applied to the development of a CE-LIF method for detection of ZEN in maize. The resulting method was capable of detecting ZEN with a limit of quantitation of 5 ng/g maize. Recoveries of ZEN from maize spiked over the range from 5 ng/g to 500 ng/g averaged 103.1+/-8.5% (n=20). The CE-LIF method will be useful for future studies of ZEN in maize.
authors.affiliation: Mycotoxin Research Unit, US Department of Agriculture-Agricultural Research Service, 1815 N. University St., Peoria, IL 61604, USA. maragocm@ncaur.usda.gov
authors.forename: Chris M
authors.initials: CM
authors.lastname: Maragos
authors.forename: Michael
authors.initials: M
authors.lastname: Appell
mesh_terms: D003505:Cyclodextrins / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D003313:Zea mays / Q000737:chemistry; D015025:Zearalenone / Q000032:analysis*
chemical_list: D003505:Cyclodextrins; D015025:Zearalenone