title: Effect of antioxidant on coronal seal of dentin following sodium-hypochlorite and hydrogen-peroxide irrigation.
abstract: OBJECTIVE
  To reduce the microleakage of a self-etching priming dentin adhesive with the use of antioxidant or bur finishing after sodium-hypochlorite or hydrogen-peroxide irrigation.
  
  
  METHODS
  70 single-root canals were enlarged and seven different treatment protocols were applied throughout the root canal treatment: The roots in Groups 1, 2, and 3 were irrigated with sodium-hypochlorite. Group 1 was used as the negative control with only sodium-hypochlorite irrigation whereas in Group 2, sodium-ascorbate was applied as an additional irrigation agent following sodium-hypochlorite. Irrigation procedure in Group 3 was same as in Group 1, however, after the roots in this group were obturated, cavities were cleaned off with a carbide bur (bur-finishing) to remove the effect of sodium-hypochlorite. Hydrogen-peroxide irrigation was used in Groups 4, 5 and 6; the procedural steps were similar to those of Groups 1, 2 and 3: hydrogen-peroxide in Group 4, sodium-ascorbate application in Group 5, and bur-finishing in Group 6. Group 7 was the positive control with saline irrigation alone. All roots were obturated with Diaket sealer and gutta-percha cones using cold lateral condensation technique immediately after irrigation. A self-etching priming adhesive plus resin composite was applied after the endodontic treatment. The microleakage of dentin margins was determined using dye-penetration technique with clearing process.
  
  
  RESULTS
  The Kruskal-Wallis followed by Mann-Whitney test showed that both sodium-hypochlorite and hydrogen-peroxide deteriorated the marginal seal of the dentin adhesive (P < 0.05), however, following both irrigation solutions the use of sodium-ascorbate reduced the microleakage (P < 0.05). Additionally, when sodium-ascorbate or bur-finishing was applied to remove the deterioration caused by sodium-hypochlorite or hydrogen-peroxide, the microleakage scores obtained were not different from that of the positive control (P > 0.05).
authors.affiliation: Department of Restorative Dentistry and Endodontics, Ege University School of Dentistry, 35100 Bornova, Izmir, Turkey. tijenpamir@yahoo.com
authors.forename: Tijen
authors.initials: T
authors.lastname: Pamir
authors.forename: Murat
authors.initials: M
authors.lastname: Türkün
authors.forename: Ayşegül Demirbaş
authors.initials: AD
authors.lastname: Kaya
authors.forename: Figen
authors.initials: F
authors.lastname: Sevgican
mesh_terms: D000975:Antioxidants / Q000627:therapeutic use*; D001205:Ascorbic Acid / Q000627:therapeutic use*; D003763:Dental Leakage / Q000209:etiology* / Q000517:prevention & control*; D003804:Dentin / Q000187:drug effects; D017220:Dentin-Bonding Agents; D006801:Humans; D006861:Hydrogen Peroxide / Q000009:adverse effects; D007180:Incisor; D012388:Root Canal Irrigants / Q000009:adverse effects*; D012389:Root Canal Obturation; D012973:Sodium Hypochlorite / Q000009:adverse effects; D018709:Statistics, Nonparametric
chemical_list: D000975:Antioxidants; D017220:Dentin-Bonding Agents; D012388:Root Canal Irrigants; D006861:Hydrogen Peroxide; D012973:Sodium Hypochlorite; D001205:Ascorbic Acid