title: High prevalence of human bocavirus detected in young children with severe acute lower respiratory tract disease by use of a standard PCR protocol and a novel real-time PCR protocol.
abstract: The human bocavirus (HBoV) was recently isolated from respiratory tract samples. Within a study collective of children with severe lower respiratory tract disease, the patients testing positive for HBoV (12.8%) had a higher rate of underlying cardiopulmonary disease. Viral loads in respiratory tract specimens varied from 10(2) to 10(10) genome equivalents/ml.
authors.affiliation: Division of Virology, Department of Medical Microbiology, University Hospital Aachen, RWTH Aachen, Pauwelsstrasse 30, D-52057 Aachen, Germany. mkleines@ukaachen.de
authors.forename: Michael
authors.initials: M
authors.lastname: Kleines
authors.forename: Simone
authors.initials: S
authors.lastname: Scheithauer
authors.forename: Annette
authors.initials: A
authors.lastname: Rackowitz
authors.forename: Klaus
authors.initials: K
authors.lastname: Ritter
authors.forename: Martin
authors.initials: M
authors.lastname: Häusler
mesh_terms: D000208:Acute Disease; D052660:Bocavirus / Q000235:genetics / Q000302:isolation & purification*; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D007223:Infant; D010322:Parvoviridae Infections / Q000453:epidemiology* / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D015995:Prevalence; D012141:Respiratory Tract Infections / Q000453:epidemiology* / Q000821:virology
chemical_list: D004279:DNA, Viral