title: Staphylococcus aureus Panton-Valentine leukocidin causes necrotizing pneumonia.
abstract: The Staphylococcus aureus Panton-Valentine leukocidin (PVL) is a pore-forming toxin secreted by strains epidemiologically associated with the current outbreak of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) and with the often-lethal necrotizing pneumonia. To investigate the role of PVL in pulmonary disease, we tested the pathogenicity of clinical isolates, isogenic PVL-negative and PVL-positive S. aureus strains, as well as purified PVL, in a mouse acute pneumonia model. Here we show that PVL is sufficient to cause pneumonia and that the expression of this leukotoxin induces global changes in transcriptional levels of genes encoding secreted and cell wall-anchored staphylococcal proteins, including the lung inflammatory factor staphylococcal protein A (Spa).
authors.affiliation: Center for Extracellular Matrix Biology, Institute of Biosciences and Technology, Texas A&M University System Health Science Center, Houston, TX 77030, USA.
authors.forename: Maria
authors.initials: M
authors.lastname: Labandeira-Rey
authors.forename: Florence
authors.initials: F
authors.lastname: Couzon
authors.forename: Sandrine
authors.initials: S
authors.lastname: Boisset
authors.forename: Eric L
authors.initials: EL
authors.lastname: Brown
authors.forename: Michele
authors.initials: M
authors.lastname: Bes
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Benito
authors.forename: Elena M
authors.initials: EM
authors.lastname: Barbu
authors.forename: Vanessa
authors.initials: V
authors.lastname: Vazquez
authors.forename: Magnus
authors.initials: M
authors.lastname: Höök
authors.forename: Jerome
authors.initials: J
authors.lastname: Etienne
authors.forename: François
authors.initials: F
authors.lastname: Vandenesch
authors.forename: M Gabriela
authors.initials: MG
authors.lastname: Bowden
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001427:Bacterial Toxins / Q000235:genetics; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D005098:Exotoxins / Q000235:genetics / Q000502:physiology*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D006470:Hemorrhage; D007956:Leukocidins / Q000235:genetics / Q000502:physiology*; D008168:Lung / Q000382:microbiology / Q000473:pathology*; D016106:Methicillin Resistance; D051379:Mice; D008807:Mice, Inbred BALB C; D009336:Necrosis; D020411:Oligonucleotide Array Sequence Analysis; D011023:Pneumonia, Staphylococcal / Q000382:microbiology* / Q000473:pathology*; D013205:Staphylococcal Protein A / Q000235:genetics / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000472:pathogenicity*; D014158:Transcription, Genetic; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D002135:Calcium-Binding Proteins; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; C115915:SdrD protein, Staphylococcus aureus; D013205:Staphylococcal Protein A; D037521:Virulence Factors