[Determination of ginsenoside rd in human plasma by LC/MS/MS].
OBJECTIVE
To develop a HPLC/MS/MS method for the determination of ginsenoside Rd in human plasma.


METHODS
Plasma samples were pretreated by solid phase extraction (SPE). Ginsenoside Rd (m/z 964.6-->m/z 767.5) and gentiopicrin (m/z 374.1-->195.1) was detected by the positive electrospray ionization (ES+I)-MS method under multiple reaction monitoring (MRM) mode.


RESULTS
The calibration curve of ginsenoside Rd in plasma was linear over the range of 3.00-5000.00 ng/ml. The limit of quantitation was 3.00 ng/ml. The relative recovery was 81.01-83.39%. The within-day and between-day RSDs were less than 15%. CONCLUSION This method is accurate, sensitive, and specific. It is suitable for the measurement of plasma ginsenoside Rd concentration.
Deng
Yuan-hui
YH
Department of Clinical Pharmacology, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510120, China.
Feng
Yi
Y
Zeng
Xing
X
Yang
Liu
L
Liu
Yi-ming
YM
Huang
Yu
Y
Sun
Jing
J
Zhou
Dan
D
Liang
Wei-xiong
WX
D019540:Area Under Curve; D001682:Biological Availability; D004355:Drug Stability; D036145:Ginsenosides / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D007275:Injections, Intravenous; D010946:Plants, Medicinal / Q000737:chemistry; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
D036145:Ginsenosides