Cytoprotective agent in Lactobacillus bulgaricus extracts.
Adenosine 5'-diphosphoribose (ADP-ribose) has been identified as a significant contributor to the anti-cytotoxic activity of Lactobacillus bulgaricus extracts. Although the biological activities associated with the administration of probiotic bacteria and components thereof are sometimes attributed to the peptidoglycans that comprise a substantial portion of the Gram-positive bacterial cell wall, we found that the beta-nicotine adenine dinucleotide (NAD) hydrolysis product ADP-ribose was a significant contributor to the observed anti-cytotoxicity in our L. bulgaricus extracts. The ADP-ribose was isolated, identified, and quantitated by high performance liquid chromatography (HPLC) and by nuclear magnetic resonance (NMR) spectroscopy. ADP-ribose levels as low as 5 mg/L exhibited a measurable inhibition of tumor necrosis factor alpha (TNF-alpha) mediated cytotoxicity in an in vitro cell assay, whereas the ADP-ribose content of the L. bulgaricus extracts often exceeded 5 mg/g dry weight.
Johns
Paul
P
Abbott Laboratories, Ross Products Division, Columbus, Ohio 43219, USA. paul.johns@abbott.com
Pereira
Suzette L
SL
Leonard
Amanda E
AE
Mukerji
Pradip
P
Shalwitz
Robert A
RA
Dowlati
Lobat
L
Phillips
Rosalyn R
RR
Bergana
Marti S
MS
Holton
Jason D
JD
Das
Tapas
T
D000246:Adenosine Diphosphate Ribose / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D003513:Cycloheximide / Q000633:toxicity; D004847:Epithelial Cells / Q000187:drug effects*; D007778:Lactobacillus / Q000378:metabolism*; D008168:Lung / Q000166:cytology*; D009682:Magnetic Resonance Spectroscopy; D019936:Probiotics / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000633:toxicity
D014409:Tumor Necrosis Factor-alpha; D000246:Adenosine Diphosphate Ribose; D003513:Cycloheximide