title: Clinical usefulness of antibodies against Babesia gibsoni detected by ELISA with recombinant P50.
abstract: The clinical usefulness of antibodies against Babesia gibsoni detected by ELISA with recombinant P50 was examined in dogs in an area where B. gibsoni infection was endemic. Only 8 among 14 dogs with acute type B. gibsoni infection without a previous history of infection were positive. This high percentage of false-negative results is thought to be a weak point of ELISA as a diagnostic tool. However, 14 other anemic dogs with a confirmed history of B. gibsoni infection were all positive, thus confirming the higher sensitivity of ELISA in detecting a history of infection.
authors.affiliation: Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Miyama
authors.forename: Hisashi
authors.initials: H
authors.lastname: Inokuma
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Itamoto
authors.forename: Masaru
authors.initials: M
authors.lastname: Okuda
authors.forename: Rodolfo A
authors.initials: RA
authors.lastname: Verdida
authors.forename: Xuenan
authors.initials: X
authors.lastname: Xuan
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000276:immunology*; D001403:Babesia / Q000276:immunology*; D001404:Babesiosis / Q000097:blood / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D004283:Dog Diseases / Q000097:blood / Q000175:diagnosis* / Q000276:immunology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005188:False Negative Reactions
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; C434466:P50 protein, Babesia gibsoni