title: Characterization of raffinose synthase from rice (Oryza sativa L. var. Nipponbare).
abstract: The putative raffinose synthase gene from rice was cloned and expressed in Escherichia coli. The enzyme displayed an optimum activity at 45 degrees C and pH 7.0, and a sulfhydryl group was required for its activity. The enzyme was specific for galactinol and p-nitrophenyl-alpha-D-galactoside as galactosyl donors, and sucrose, lactose, 4-beta-galactobiose, N-acetyl-D-lactosamine, trehalose and lacto-N-biose were recognized as galactosyl acceptors.
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
authors.forename: Suhong
authors.initials: S
authors.lastname: Li
authors.forename: Tuoping
authors.initials: T
authors.lastname: Li
authors.forename: Wook-Dong
authors.initials: WD
authors.lastname: Kim
authors.forename: Motomitsu
authors.initials: M
authors.lastname: Kitaoka
authors.forename: Shigeki
authors.initials: S
authors.lastname: Yoshida
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Nakajima
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Kobayashi
mesh_terms: D004789:Enzyme Activation; D004795:Enzyme Stability; D005700:Galactosyltransferases / Q000737:chemistry*; D012275:Oryza / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D005700:Galactosyltransferases; C468749:raffinose synthase