title: Microcosm evaluation of bioaugmentation after field-scale thermal treatment of a TCE-contaminated aquifer.
abstract: This paper investigates effects of combining thermal and biological remediation, based on laboratory studies of trichloroethene (TCE) degradation. Aquifer material was collected 6 months after terminating a full-scale Electrical Resistance Heating (ERH), when the site had cooled from approximately 100 degrees C to 40 degrees C. The aquifer material was used to construct bioaugmented microcosms amended with the mixed anaerobic dechlorinating culture, KB-1(TM), and an electron donor (5 mM lactate). Microcosms were bioaugmented during cooling at 40, 30, 20, and 10 degrees C, as temperatures continually decreased during laboratory incubation. Redox conditions were generally methanogenic, and electron donors were present to support dechlorination. For microcosms bioaugmented at 10 degrees C and 20 degrees C, dechlorination stalled at cis-dichloroethene (cDCE) and vinyl chloride (VC) 150 days after bioaugmentation. However, within 300 days of incubation ethene was produced in the majority of these microcosms. In contrast, dechlorination was rapid and complete in microcosms bioaugmented at 30 degrees C. Microcosms bioaugmented at 40 degrees C also showed rapid dechlorination, but stalled at cDCE with partial VC and ethene production, even after 150 days of incubation when the temperature had decreased to 10 degrees C. These results suggest that sequential bioremediation of TCE is possible in field-scale thermal treatments after donor addition and bioaugmentation and that the optimal bioaugmentation temperature is approximately 30 degrees C. When biological and thermal remediations are to be applied at the same location, three bioremediation approaches could be considered: (a) treating TCE in perimeter areas outside the source zone at temperatures of approximately 30 degrees C; (b) polishing TCE concentrations in the original source zone during cooling from approximately 30 degrees C to ambient groundwater temperatures; and (c) using bioremediation in downgradient areas taking advantages of the higher temperature and potential release of organic matter.
authors.affiliation: Institute of Environment & Resources, Technical University of Denmark, Building 115, Bygningstorvet, 2800 Kgs. Lyngby, Denmark. akf@er.dtu.dk
authors.forename: Anne Kirketerp
authors.initials: AK
authors.lastname: Friis
authors.forename: Julie L L
authors.initials: JL
authors.lastname: Kofoed
authors.forename: Gorm
authors.initials: G
authors.lastname: Heron
authors.forename: Hans-Jørgen
authors.initials: HJ
authors.lastname: Albrechtsen
authors.forename: Poul L
authors.initials: PL
authors.lastname: Bjerg
mesh_terms: D000693:Anaerobiosis; D001419:Bacteria / Q000254:growth & development; D001673:Biodegradation, Environmental; D002713:Chlorine / Q000302:isolation & purification; D004579:Electron Transport; D005285:Fermentation; D007700:Kinetics; D010084:Oxidation-Reduction; D013696:Temperature; D014241:Trichloroethylene / Q000302:isolation & purification* / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000302:isolation & purification* / Q000378:metabolism*; D018508:Water Purification / Q000379:methods; D014881:Water Supply
chemical_list: D014874:Water Pollutants, Chemical; D014241:Trichloroethylene; D002713:Chlorine