Diagnosing viruses by the rolling circle amplified synthesis of DNAzymes.
Circular DNA is used as a template for the amplified detection of M13 phage ssDNA by a rolling circle amplification (RCA) process that synthesizes DNAzyme chains, thus enabling the colorimetric or chemiluminescent detection of the analyte.
Cheglakov
Zoya
Z
Institute of Chemistry and The Farkas Center for Light-Induced Processes, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel.
Weizmann
Yossi
Y
Basnar
Bernhard
B
Willner
Itamar
I
D017104:Bacteriophage M13 / Q000235:genetics; D001483:Base Sequence; D003124:Colorimetry / Q000379:methods; D004247:DNA / Q000737:chemistry; D004267:DNA Viruses / Q000235:genetics*; D021881:DNA, Catalytic / Q000737:chemistry* / Q000378:metabolism; D004270:DNA, Circular / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D006861:Hydrogen Peroxide / Q000737:chemistry; D049449:Luminescence; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010100:Oxygen / Q000737:chemistry; D013997:Time Factors
D021881:DNA, Catalytic; D004270:DNA, Circular; D004277:DNA, Single-Stranded; D004247:DNA; D006861:Hydrogen Peroxide; D010100:Oxygen