title: Cytogenetic analysis of human bone marrow-derived mesenchymal stem cells passaged in vitro.
abstract: This study concerns the cytogenetic stability of in vitro human bone marrow-derived mesenchymal stem cells (MSCs) in primary culture and after passaging. Bone marrow samples were collected from seven brain malfunction patients involved in autologous MSC transplantation trials. Chromosome preparations from primary MSC cultures and after 3 passages were analyzed by conventional staining and G-banding techniques. All MSCs showed normal diploid karyotypes, 46 XY or 46 XX, without aneuploidy or polyploidy; chromosome structural abnormalities were not detected. The results indicate that the in vitro cultured MSCs retained normal cytogenetics before being transplanted back into the patients.
authors.affiliation: Department of Neurosurgery, Weifang People's Affiliated Hospital, Weifang Medical College, Weifang 261041, PR China.
authors.forename: Zhen-Xing
authors.initials: ZX
authors.lastname: Zhang
authors.forename: Li-Xue
authors.initials: LX
authors.lastname: Guan
authors.forename: Kun
authors.initials: K
authors.lastname: Zhang
authors.forename: Shouxian
authors.initials: S
authors.lastname: Wang
authors.forename: Pei-Cheng
authors.initials: PC
authors.lastname: Cao
authors.forename: Yong-He
authors.initials: YH
authors.lastname: Wang
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.forename: Long-Jun
authors.initials: LJ
authors.lastname: Dai
mesh_terms: D000328:Adult; D001854:Bone Marrow Cells / Q000166:cytology*; D002478:Cells, Cultured; D002675:Child, Preschool; D002871:Chromosome Banding; D002877:Chromosomes, Human / Q000235:genetics; D020732:Cytogenetic Analysis / Q000379:methods*; D005260:Female; D006801:Humans; D007621:Karyotyping; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008677:Metaphase; D008875:Middle Aged