Prevalence of Helicobacter equorum in faecal samples from horses and humans.
Recently, a new enterohepatic Helicobacter species, H. equorum, was isolated from faecal samples of two clinically healthy horses. At the onset of this study, nothing was known about the prevalence of this organism in horses, nor was there any information available on the possible zoonotic character of this agent. This study aimed to determine the prevalence of H. equorum in faecal samples from equine and human origin. Therefore, faecal samples of 120 healthy privately owned horses, 227 healthy riding-school horses and 239 hospitalised horses were screened for H. equorum-DNA by means of a PCR amplifying a 1074-bp fragment of the 23S rRNA gene with primers specific for H. equorum. The vast majority of the hospitalised horses were under treatment with an antimicrobial agent at the moment of sampling, while the other horses had not been treated with an antimicrobial agent in the 14 days preceding the sampling. Stool samples of 531 humans suffering from gastro-intestinal disease and 100 clinically healthy humans were likewise examined. H. equorum-DNA was demonstrated in faeces from 0.8% of the privately owned horses, 3.1% of the riding-school horses and 7.9% of the hospitalised horses. The prevalence of H. equorum was significantly higher in hospitalised than in healthy, privately owned horses (P=0.02). H. equorum-DNA was not detected in human samples. These results indicate that the prevalence of H. equorum in horses may be influenced by the health status of the investigated horse population and/or by antimicrobial treatment. We may additionally assume that this micro-organism does not commonly infect humans.
Moyaert
H
H
Department of Pathology, Bacteriology and Avian Diseases, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. Hilde.Moyaert@UGent.be
Haesebrouck
F
F
Baele
M
M
Picavet
T
T
Ducatelle
R
R
Chiers
K
K
Ceelen
L
L
Decostere
A
A
D000818:Animals; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D005243:Feces / Q000382:microbiology; D005767:Gastrointestinal Diseases / Q000382:microbiology* / Q000662:veterinary*; D016998:Helicobacter / Q000235:genetics / Q000302:isolation & purification*; D016481:Helicobacter Infections / Q000382:microbiology* / Q000662:veterinary*; D006734:Horse Diseases / Q000382:microbiology*; D006736:Horses; D006801:Humans; D016015:Logistic Models; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D012338:RNA, Ribosomal, 23S / Q000737:chemistry / Q000235:genetics; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial; D012338:RNA, Ribosomal, 23S