title: Photogenerated glycan arrays identify immunogenic sugar moieties of Bacillus anthracis exosporium.
abstract: Using photogenerated glycan arrays, we characterized a large panel of synthetic carbohydrates for their antigenic reactivities with pathogen-specific antibodies. We discovered that rabbit IgG antibodies elicited by Bacillus anthracis spores specifically recognize a tetrasaccharide chain that decorates the outermost surfaces of the B. anthracis exosporium. Since this sugar moiety is highly specific for the spores of B. anthracis, it appears to be a key biomarker for detection of B. anthracis spores and development of novel vaccines that target anthrax spores.
authors.affiliation: Carbohydrate Microarray Laboratory, Stanford University School of Medicine, Stanford, CA, USA. dwang1@stanford.edu
authors.forename: Denong
authors.initials: D
authors.lastname: Wang
authors.forename: Gregory T
authors.initials: GT
authors.lastname: Carroll
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Turro
authors.forename: Jeffrey T
authors.initials: JT
authors.lastname: Koberstein
authors.forename: Pavol
authors.initials: P
authors.lastname: Kovác
authors.forename: Rina
authors.initials: R
authors.lastname: Saksena
authors.forename: Roberto
authors.initials: R
authors.lastname: Adamo
authors.forename: Leonore A
authors.initials: LA
authors.lastname: Herzenberg
authors.forename: Leonard A
authors.initials: LA
authors.lastname: Herzenberg
authors.forename: Lawrence
authors.initials: L
authors.lastname: Steinman
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology*; D001408:Bacillus anthracis / Q000737:chemistry / Q000276:immunology*; D008027:Light; D046228:Microarray Analysis; D011134:Polysaccharides / Q000737:chemistry / Q000276:immunology*; D011817:Rabbits; D013171:Spores, Bacterial / Q000737:chemistry / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D011134:Polysaccharides