Endosperm tissue is good production platform for artificial recombinant proteins in transgenic rice.
Transgenic rice plants expressing 7Crp peptide were generated by Agrobacterium-mediated transformation. The 7Crp peptide is the hybrid peptide of seven major human T-cell epitopes derived from Japanese cedar pollen allergens Cry j 1 and Cry j 2. When the 7Crp gene was expressed under the control of the rice AGPase large subunit or maize ubiquitin-1 promoters, it could only be detected in the endosperm of rice seed, although high levels of RNA transcript were observed in the leaf, stem, and seed embryo. It was demonstrated by confocal and electron microscopy analysis that the 7Crp peptide was mainly localized in the endoplasmic reticulum-derived protein bodies, designated protein body I (PB-I). Our results indicate that rice endosperm tissue has advantage over other tissues as a production platform for foreign recombinant proteins.
Takaiwa
Fumio
F
Transgenic Crop Research and Development Center, National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba Ibaraki 305-8602, Japan. takaiwa@nias.affrc.go.jp
Takagi
Hidenori
H
Hirose
Sakiko
S
Wakasa
Yuhya
Y
D000485:Allergens; D001426:Bacterial Proteins / Q000235:genetics*; D012275:Oryza / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D011058:Pollen; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012231:Rhizobium / Q000235:genetics*; D014197:Trees; D025801:Ubiquitin / Q000235:genetics; D003313:Zea mays / Q000235:genetics
D000485:Allergens; D001426:Bacterial Proteins; D010940:Plant Proteins; D011994:Recombinant Proteins; D025801:Ubiquitin