Inhibitory effect of pulmonary carcinoma by adenovirus-mediated CD/UPRT gene.
The cell killing effects and bystander effects of double suicide gene on pulmonary carcinoma cells were explored. Lung adenocarcinoma cells (A549) were transfected with different titers of adenovirus vector and followed with different concentrations of 5-FC after a recombinant adenovirus vector carrying CD/UPRT gene (Ad-CD/UPRT) was constructed. The cell viability was measured by MTT assay 4 days later. The cell viability was dropped to 30.57 %-8.62 % after 10 MOI of Ad-CD/UPRT transfected and 5-FC (10-1000 microg/mL) administration. Furthermore, Ad-CD/UPRT-infected A549 cells showed a profound neighbor cell killing effect in the same methods. These results suggested that Ad-CD/UPRT/5-FC system can effectively suppress growth of lung adenocarcinoma cells, which may provide a novel and powerful candidate for lung cancer gene therapy strategies.
Huang
Qi
Q
Department of Thoracic and Cardiac Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Chen
Dayu
D
Fu
Xiangning
X
Zu
Yukun
Y
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000256:Adenoviridae / Q000235:genetics* / Q000378:metabolism; D024201:Bystander Effect; D045744:Cell Line, Tumor; D002470:Cell Survival; D043525:Cytosine Deaminase / Q000096:biosynthesis / Q000235:genetics; D005472:Fluorouracil / Q000494:pharmacology; D041722:Genes, Transgenic, Suicide; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D010430:Pentosyltransferases / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection
D011994:Recombinant Proteins; D010430:Pentosyltransferases; C023269:uracil phosphoribosyltransferase; D043525:Cytosine Deaminase; D005472:Fluorouracil