title: Relationship between bone ingrowth, mineral apposition rate, and osteoblast activity.
abstract: To better understand skeletal attachment of porous coated total hip and knee implants over time, this study investigated the dynamics of osteoblast populations at the interface of porous coated implants in a weight-bearing ovine model. The relationship between cancellous bone ingrowth, mineral apposition rate (MAR), and osteoblast activity indicators such as osteoblast area, relative osteoblast number, osteoid width, and osteoid area (O.Ar.) were investigated. The data demonstrated that the percent O.Ar. was a marginally significant predictor of bone ingrowth and MAR over time, suggesting that the amount of osteoid present influenced bone ingrowth and MAR in the porous coated implants. The data also demonstrated that all osteoblast activity indicators were significantly greater in the porous coated region compared to the host bone region, while controlling for in situ time (p < 0.05). This may have been due to the trauma of implantation or the influence of the implant load on the bone tissue promoting a regional acceleratory phenomenon. The localized response suggests that specific therapies may be developed to affect the physiology of osteoblasts at the interface of implants, which may allow for improve skeletal attachment of biomaterials and clinical outcomes of cementless joint replacements.
authors.affiliation: Bone and Joint Research Lab, Research Service, VA SLC Health Care System, 500 Foothill Blvd., Salt Lake City, Utah 84148, USA. roy.bloebaum@hsc.utah.edu
authors.forename: R D
authors.initials: RD
authors.lastname: Bloebaum
authors.forename: B M
authors.initials: BM
authors.lastname: Willie
authors.forename: B S
authors.initials: BS
authors.lastname: Mitchell
authors.forename: A A
authors.initials: AA
authors.lastname: Hofmann
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D006801:Humans; D066298:In Vitro Techniques; D007595:Joint Prosthesis; D016014:Linear Models; D008422:Materials Testing; D008903:Minerals / Q000378:metabolism; D015999:Multivariate Analysis; D016348:Osseointegration / Q000502:physiology*; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D012756:Sheep
chemical_list: D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D008903:Minerals