[Inflammatory mediators and hematological parameters in cord blood in labor complicated by meconium-stained amniotic fluid].
OBJECTIVE
Meconium-stained amniotic fluid at term gestation is a predictor for adverse perinatal outcome and is associated with increased peripartum infections, independent of other risk factors. The aim of our study was to evaluate concentrations of inflammatory mediators (such as cytokine IL-6 and intracellular adhesion molecule ICAM-1) and values of hematological parameters of cord blood in presence or absence of meconium in amniotic fluid in term labor.


METHODS
Cord blood samples were obtained from 66 term normal neonates immediately after birth, Soluble ICAM-1 and IL-6 concentrations were measured with ELISA R&D Systems kits. The umbilical blood specimen was analyzed using an automated hematology cell analyzer. Blood films were stained using May-Grünwald-Giemsa method.


RESULTS
There were no difference in concentrations of ICAM-1 and IL-6 in cord blood in groups with or without meconium-stained amniotic fluid. The mean count of umbilical nucleated red blood cells and white blood cells was significantly higher in meconium group. There was no correlation between the cord blood hematological values and ICAM-1 or IL-6. There was also no correlation between IL-6 and ICAM-I and duration of labor. The mode of delivery influenced cord blood IL-6 levels.


CONCLUSIONS
There was no influence of meconium-stained amniotic fluid on cord blood IL-6 and ICAM-1 levels. Changes in hematological parameters in cord blood in meconium passage can suggest either fetus hypoxia or infection. Significant differences of concentrations of fetal IL-6 were associated with the mode of delivery.
Redźko
Sławomir
S
Klinika Perinatologii Akademii Medycznej w Białymstoku. redzkosl@amb.edu.pl
Przepieść
Jerzy
J
Zelazowska
Beata
B
Zak
Janusz
J
Wysocka
Jolanta
J
Urban
Jan
J
D000653:Amniotic Fluid / Q000737:chemistry / Q000276:immunology*; D015331:Cohort Studies; D036861:Delivery, Obstetric; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005312:Fetal Blood / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D007231:Infant, Newborn; D018836:Inflammation Mediators / Q000378:metabolism*; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D015850:Interleukin-6 / Q000032:analysis; D007743:Labor, Obstetric / Q000276:immunology*; D008297:Male; D008470:Meconium / Q000737:chemistry / Q000276:immunology*; D011247:Pregnancy
D018836:Inflammation Mediators; D015850:Interleukin-6; D018799:Intercellular Adhesion Molecule-1