title: Injury of skeletal muscle and specific cytokines induce the expression of gap junction channels in mouse dendritic cells.
abstract: Dendritic cells (DCs) in culture express at least connexin43, a protein subunit of gap junctions, and form gap junction channels, which could be important for T-cells activation. Here, we evaluated whether DCs express connexins in vivo and also to identify components of their microenvironment that regulate the functional expression of gap junctions. In vivo studies were performed in lymph nodes of mice under control conditions or after skeletal muscle damage. In double immunolabeling studies, connexin45 was frequently detected in DEC205(+) DCs in lymph nodes of control animals, whereas connexin43 was rarely found in DCs. However, connexin43 was upregulated in DCs after skeletal muscle damage. Upregulation of connexin43 gene expression by tissue damage was also confirmed in mice carrying a beta-galactosidase reporter gene in a connexin43 allele. The effect of several cytokines on the expression of functional gap junctions between cultured DCs was also tested. Under control conditions, cultured DCs did not communicate via gap junctions. However, after treatment with keratinocyte-conditioned medium or cytokine mixtures containing at least TNF-alpha and IL-1beta, they became transiently coupled through a pathway sensitive to octanol, a gap junction blocker. Cellular coupling induced by effective cytokine mixtures was prevented by IL-6. Single cytokines (TNF-alpha, IL-1beta, IFN-gamma, or IL-6) or other mixtures than the described above did not induce coupling via gap junctions. Increased levels of connexin43 and connexin45 protein and mRNA accompanied the appearance of cellular coupling. These studies provide demonstration of connexin expression and regulation by specific danger signals in DCs.
authors.affiliation: Departamento de Ciencias Fisiológicas, Pontificia Universidad Católica de Chile, Santiago, Chile.
authors.forename: Liliana A
authors.initials: LA
authors.lastname: Corvalán
authors.forename: Roberto
authors.initials: R
authors.lastname: Araya
authors.forename: María C
authors.initials: MC
authors.lastname: Brañes
authors.forename: Pablo J
authors.initials: PJ
authors.lastname: Sáez
authors.forename: Alexis M
authors.initials: AM
authors.lastname: Kalergis
authors.forename: Jaime A
authors.initials: JA
authors.lastname: Tobar
authors.forename: Martin
authors.initials: M
authors.lastname: Theis
authors.forename: Klaus
authors.initials: K
authors.lastname: Willecke
authors.forename: Juan C
authors.initials: JC
authors.lastname: Sáez
mesh_terms: D000818:Animals; D002450:Cell Communication / Q000187:drug effects / Q000276:immunology; D002460:Cell Line; D018031:Connexin 43 / Q000235:genetics*; D017630:Connexins / Q000235:genetics*; D017077:Culture Media, Conditioned / Q000494:pharmacology; D016207:Cytokines / Q000494:pharmacology*; D003713:Dendritic Cells / Q000166:cytology / Q000502:physiology*; D017629:Gap Junctions / Q000502:physiology; D007371:Interferon-gamma / Q000494:pharmacology; D053583:Interleukin-1beta / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D015603:Keratinocytes / Q000166:cytology; D008198:Lymph Nodes / Q000166:cytology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D018482:Muscle, Skeletal / Q000276:immunology* / Q000293:injuries*; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D015854:Up-Regulation / Q000502:physiology
chemical_list: D018031:Connexin 43; D017630:Connexins; D017077:Culture Media, Conditioned; D016207:Cytokines; D053583:Interleukin-1beta; D015850:Interleukin-6; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C082920:connexin 45; D007371:Interferon-gamma