Effect of rescuing donated immature human oocytes derived after FSH/hCG stimulation following in vitro culture with or without Follicular Fluid Meiosis Activating Sterol (FF-MAS)--an embryo chromosomal and morphological analysis.
OBJECTIVE
Studies in mice and humans have shown that Follicular Fluid - Meiosis Activating Sterol (FF-MAS) induces meiotic maturation of immature oocytes in vitro. A multicenter, prospectively randomised study evaluated chromosomal status of embryos from FSH/hCG primed human immature oocytes, cultured with or without FF-MAS.


METHODS
Denuded immature oocytes (n=365) were randomly allocated into inert control, FF-MAS 5 microM or 20 microM. Seventy +/-2 hours after ICSI on matured oocytes, all cleaved embryos were fixed for fluorescence in situ hybridisation analysis.


RESULTS
Only 15% of oocytes resulted in cleaved embryos. GV oocytes matured at significantly lower rates (14% and 7%) in the two FF-MAS groups compared to the inert control group (47%). High rates of chromosomal abnormalities were found in all groups.


CONCLUSIONS
Immature oocytes showed poor development with high rates of embryo chromosomal abnormalities. Exposure to FF-MAS in the concentrations, duration and/or formulation used in this study did not improve the results.
Lundin
Kersti
K
Department of Obstetrics and Gynecology, Reproductive Medicine, Sahlgrenska University Hospital, Göteborg, Sweden. kersti.lundin@vgregion.se
Ziebe
Søren
S
Bergh
Christina
C
Loft
Anne
A
Selleskog
Ulrika
U
Nilsson
Lars
L
Grøndahl
Christian
C
D000818:Animals; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D002782:Cholestenes / Q000494:pharmacology*; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D002869:Chromosome Aberrations; D020732:Cytogenetic Analysis; D004622:Embryo, Mammalian / Q000166:cytology*; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006801:Humans; D051379:Mice; D018587:Oocyte Donation; D009865:Oocytes / Q000187:drug effects*
D002782:Cholestenes; D006063:Chorionic Gonadotropin; C100324:4,4-dimethylcholesta-8,14,24-trienol; D005640:Follicle Stimulating Hormone