Preparation and physicochemical properties of scaffold materials of heterogeneous deproteinized bone.
OBJECTIVE
To prepare and observe the physicochemical properties of scaffold materials of heterogeneous deproteinized tissue-engineered bone.


METHODS
Deproteinized bone was made through a series of physicochemical treatments in pig ribs and analyzed with histological observation, scanning electron microscopy, infrared spectrum, X-ray diffraction and energy dispersive analysis, Kjeldahl determination and mechanics analysis.


RESULTS
Interstitial collagen fiber was positive and mucin was negative in deproteinized bone, but, both were positive in fresh bone. Deproteinized bone maintained natural pore network. Its pore size was 472.51 micromolar+/-7.02 micromolar and the porosity was 78.15%+/-6.45%. The results of infrared spectrum showed that collagen was present in deproteinized bone. Both fresh and deproteinized bone had curve of hydroxyapatite. The Ca/P ratios were 1.71+/-0.95 and 1.68+/-0.76 (P larger than 0.05), and the protein contents were 26.6%+/-2.23% and 19.1%+/-2.14% (P less than 0.05) in fresh and deproteinized bone, respectively. There was no significant difference of destruction load under compression and maximal destruction load between fresh and deproteinized bone (P larger than 0.05). The elastic modulus was higher in deproteinized bone than that in fresh bone (P less than 0.05).


CONCLUSIONS
Physicochemical properties and mechanic strength of deproteinized tissue-engineered bone meet the demands of ideal scaffold materials. But, its immunogenicity should be observed through further experiments for its clinical applications.
Liu
Lei
L
Center for Joint Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, China. Leilei-0452@sina.com
Li
Qi-hong
QH
Tang
Kang-lai
KL
Yang
Liu
L
Jian
Yue-kui
YK
D000818:Animals; D001696:Biomechanical Phenomena; D001842:Bone and Bones / Q000737:chemistry / Q000502:physiology*; D006882:Hydroxyapatites; D008422:Materials Testing; D013552:Swine; D023822:Tissue Engineering
D006882:Hydroxyapatites