Mitoproteome plasticity of rat brown adipocytes in response to cold acclimation.
Cold acclimation induces an adaptative increase in respiration in brown adipose tissue (BAT). A comparative analysis by two-dimensional differential in-gel electrophoresis of mitochondrial protein patterns found in rat control and cold-acclimated BAT was performed. A total of 58 proteins exhibiting significant differences in their abundance was unambiguously identified. Proteins implicated in the major catabolic pathways were up-regulated as were ATP synthase and mitofilin. Moreover, these results support the fact that adipocytes can balance their ATP synthesis and their heat production linked to UCP1-sustained uncoupling.
Navet
Rachel
R
Laboratory of Bioenergetics, and Centre de Recherche en Neurobiologie Cellulaire et Moléculaire, Liège, Belgium.
Mathy
Gregory
G
Douette
Pierre
P
Dobson
Rowan Laura
RL
Leprince
Pierre
P
De Pauw
Edwin
E
Sluse-Goffart
Claudine
C
Sluse
Francis E
FE
D025181:ATP Synthetase Complexes / Q000096:biosynthesis; D000064:Acclimatization; D017667:Adipocytes / Q000378:metabolism*; D002001:Adipose Tissue, Brown / Q000166:cytology*; D000818:Animals; D001833:Body Temperature Regulation; D003080:Cold Temperature; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D007473:Ion Channels / Q000096:biosynthesis; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000096:biosynthesis; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D000071256:Uncoupling Protein 1
D007473:Ion Channels; D024101:Mitochondrial Proteins; C000604373:Ucp1 protein, rat; D000071256:Uncoupling Protein 1; D025181:ATP Synthetase Complexes