Shotgun electroelution: a proteomic tool for simultaneous sample elution from whole SDS-polyacrylamide gel slabs.
A high-throughput device has been constructed which allows parallel electroelution of separated SDS-protein bands directly from intact unsectioned polyacrylamide gel slabs as well as single electroelution of certain protein spots into a 384-well standard flat-bottom multiwell plate. The prototype provides complete, quick elution for proteomics from 1-D or from 2-D gels without gel sectioning. Since the elution chamber matrix requires no assembly, sample handling can be easily carried out by existing robotic workstations. The current design is a good candidate for automation of spot elution since there are no moving liquid containing components in the apparatus. Eight SDS-proteins were eluted in test runs and an average 70% sample recovery was achieved by re-electrophoresis of the electro-eluates.
Antal
József
J
Section on Metabolic Analysis and Mass Spectrometry, Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.
Bányász
Borbála
B
Buzás
Zsuzsanna
Z
D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D004563:Electrochemistry / Q000379:methods*; D015180:Electrophoresis, Gel, Two-Dimensional; D011506:Proteins / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry
D000180:Acrylic Resins; D011506:Proteins; C016680:polyacrylamide gels; D012967:Sodium Dodecyl Sulfate