Synaptic vesicles recycling spontaneously and during activity belong to the same vesicle pool.
Recently, it has been claimed that vesicles recycling spontaneously and during activity belong to different pools. Here we simultaneously measured, using spectrally separable styryl dyes, the release kinetics of vesicles recycled spontaneously or upon stimulation and the effects of the v-ATPase blocker folimycin on the frequency of miniature postsynaptic currents in rat hippocampal neurons. Our results provide evidence as to the identities of the vesicle pools recycling at rest and during stimulation.
Groemer
Teja W
TW
Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Goettingen, Germany.
Klingauf
Jurgen
J
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D004558:Electric Stimulation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006624:Hippocampus / Q000378:metabolism* / Q000648:ultrastructure; D018942:Macrolides / Q000494:pharmacology; D008561:Membrane Fusion / Q000187:drug effects / Q000502:physiology; D018408:Patch-Clamp Techniques; D017729:Presynaptic Terminals / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds; D051381:Rats; D013570:Synaptic Membranes / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology*; D013572:Synaptic Vesicles / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D025262:Vacuolar Proton-Translocating ATPases / Q000037:antagonists & inhibitors / Q000378:metabolism
D004791:Enzyme Inhibitors; C073804:FM1 43; D018942:Macrolides; C508379:N-(3-trimethylammoniumpropyl)-4-(6-(4-(diethylamino)phenyl)hexatrienyl)pyridinium; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds; C036978:concanamycin A; D000255:Adenosine Triphosphate; D025262:Vacuolar Proton-Translocating ATPases