title: Impact of recipient HLA-C in liver transplant: a protective effect of HLA-Cw*07 on acute rejection.
abstract: The involvement of the human leukocyte antigen (HLA) in liver graft acceptance is controversial, but the frequency of acute rejection (AR) remains high in spite of the use of the modern immunosuppressive agents. The present study was aimed at determining whether an association exists between liver recipient HLA-C polymorphism and AR development that could influence graft acceptance. Four hundred and forty-six liver recipients and 473 controls were studied within the framework of a collaborative study carried out by the Spanish Transplant Immunotolerance Group (RED-GIT). HLA-A and -B were typed by the standard microlymphocytotoxicity technique, and HLA-C by polymerase chain reaction-sequence-specific oligonucleotide probes (PCR-SSOP). A statistically significant decrease in the HLA-Cw*07 allele frequency was found in liver recipients suffering AR episodes compared to those without AR (NAR). Studies regarding the possible influence of the Asn80 and Lys80 epitopes showed that the Asn80 epitope also could be associated with AR. However, further analysis considering Asn80 alleles others than HLA-Cw*07, confirmed that the apparent protective effect of the Asn80 epitope was actually from the HLA-Cw*07 allele. In conclusion, the HLA-Cw*07 allele carried by the liver recipient is negatively associated with AR development, and could be considered a predictive factor for liver graft acceptance.
authors.affiliation: Immunology Service, University Hospital Virgen de la Arrixaca, 30120 El Palmar, Murcia, Spain.
authors.forename: Maria R
authors.initials: MR
authors.lastname: Moya-Quiles
authors.forename: Ruben
authors.initials: R
authors.lastname: Alvarez
authors.forename: Manuel
authors.initials: M
authors.lastname: Miras
authors.forename: Jorge
authors.initials: J
authors.lastname: Gomez-Mateo
authors.forename: Maria R
authors.initials: MR
authors.lastname: Lopez-Alvarez
authors.forename: Isabel
authors.initials: I
authors.lastname: Marin-Moreno
authors.forename: Enrique
authors.initials: E
authors.lastname: Martínez-Barba
authors.forename: Maria P S
authors.initials: MP
authors.lastname: Sanchez-Mozo
authors.forename: Manuel
authors.initials: M
authors.lastname: Gomez
authors.forename: Francisco
authors.initials: F
authors.lastname: Arnal
authors.forename: Francisco
authors.initials: F
authors.lastname: Sanchez-Bueno
authors.forename: Luis A
authors.initials: LA
authors.lastname: Marin
authors.forename: Ana M
authors.initials: AM
authors.lastname: Garcia-Alonso
authors.forename: Alfredo
authors.initials: A
authors.lastname: Minguela
authors.forename: Manuel
authors.initials: M
authors.lastname: Muro
authors.forename: Pascual
authors.initials: P
authors.lastname: Parrilla
authors.forename: Clara
authors.initials: C
authors.lastname: Alonso
authors.forename: Maria R
authors.initials: MR
authors.lastname: Alvarez-López
mesh_terms: D000208:Acute Disease; D000483:Alleles; D005260:Female; D006084:Graft Rejection / Q000235:genetics / Q000276:immunology* / Q000517:prevention & control*; D015236:HLA-C Antigens / Q000008:administration & dosage* / Q000235:genetics* / Q000502:physiology; D006801:Humans; D016031:Liver Transplantation / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D012189:Retrospective Studies
chemical_list: D015236:HLA-C Antigens