title: Serological and molecular evidence that canine respiratory coronavirus is circulating in Italy.
abstract: Canine respiratory coronavirus (CRCoV) is a group II coronavirus that was firstly identified in lung samples of dogs with canine infectious respiratory disease (CIRD) in UK in 2003. We report for the first time the identification of CRCoV in Italy, together with serological evidence that the virus has been circulating in the Italian dog population as from 2005. Serological investigations on 216 dog sera, carried out by an ELISA test using the strictly related bovine coronavirus (BCoV) as antigen, revealed an overall CRCoV seroprevalence of 32.06% in the last 2 years. RT-PCR targeting the S-gene of CRCoV was carried out on 109 lung samples collected from carcasses of dogs submitted for diagnostic investigations. Positive results were obtained from the lungs of a dog of the Apulia region that was co-infected with canine parvovirus type 2. Sequence analysis of the S-gene fragment amplified by RT-PCR (595bp) showed similarity to group II coronaviruses, with the highest nucleotide identity (98%) to the only CRCoV strain currently available in the GenBank database (strain T101). The results of the present study show that CRCoV is present also in continental Europe, although further studies are required to determine the real pathogenic potential of the virus.
authors.affiliation: Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy. n.decaro@veterinaria.uniba.it
authors.forename: Nicola
authors.initials: N
authors.lastname: Decaro
authors.forename: Costantina
authors.initials: C
authors.lastname: Desario
authors.forename: Gabriella
authors.initials: G
authors.lastname: Elia
authors.forename: Viviana
authors.initials: V
authors.lastname: Mari
authors.forename: Maria Stella
authors.initials: MS
authors.lastname: Lucente
authors.forename: Paolo
authors.initials: P
authors.lastname: Cordioli
authors.forename: Maria Loredana
authors.initials: ML
authors.lastname: Colaianni
authors.forename: Vito
authors.initials: V
authors.lastname: Martella
authors.forename: Canio
authors.initials: C
authors.lastname: Buonavoglia
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D018352:Coronavirus Infections / Q000453:epidemiology* / Q000662:veterinary* / Q000821:virology; D017939:Coronavirus, Canine / Q000235:genetics / Q000302:isolation & purification*; D004283:Dog Diseases / Q000453:epidemiology* / Q000821:virology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D007558:Italy / Q000453:epidemiology; D008171:Lung Diseases / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016036:Seroepidemiologic Studies
chemical_list: D000914:Antibodies, Viral; D012367:RNA, Viral