title: [Determination of plumbagin in different parts of Plumbago zeylanica by RP-HPLC].
abstract: OBJECTIVE
  To develop a RP-HPLC method to determine plumbagin in Plumbago zeylanica, and to investigate contents of plumbagin in different parts of. P. zeylanica.
  
  
  METHODS
  The analysis was carried out at 30 degrees C on a Kromasil C18, column eluted with a mobile phase consisting of a mixture of methanol-water (65: 35). The flow rate was 1 mL x min(-1), the detector wavelength was 213 nm.
  
  
  RESULTS
  The calibration curve was linear within the concentration ranges of 0.020 8-0. 104 microg (r = 0. 9999). The average recovery was 98.7%. The contents in the root, stem and leaf were 0.394 5%, 0.050 8%, 0.031 4% respectively.
  
  
  CONCLUSIONS
  This method is simple, accurate, replicate and suitable for the determination of plumbagin in P. zeylanica.
authors.affiliation: Ethnic Pharmaceutical Institute of Southwest University for Nationalities, Chengdu 610041, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Fang
authors.initials: F
authors.lastname: Deng
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.forename: Qing-Yan
authors.initials: QY
authors.lastname: Meng
authors.forename: Ze-Wen
authors.initials: ZW
authors.lastname: Gao
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009285:Naphthoquinones / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D029606:Plumbaginaceae / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results
chemical_list: D009285:Naphthoquinones; C014758:plumbagin