Integrin-dependent cell behavior on ECM peptide-conjugated chitosan membranes.
Extracellular matrix (ECM) plays an important role in tissue regeneration by promoting cell adhesion, migration, proliferation, and differentiation. ECM mimetics are of importance for tissue engineering because of their functions as scaffolds for cells. Previously, we developed bioactive laminin-derived peptide-conjugated chitosan membranes and demonstrated their cell- and peptide-type specific functions. Here, we conjugated twelve integrin-binding peptides derived from ECM proteins onto chitosan membranes and examined biological activity. Seven peptide-chitosan membranes promoted human foreskin fibroblast attachment. Additionally, FIB1 (YAVTGRGDSPAS; from fibronectin), A99 (AGTFALRGDNPQG; from laminin alpha1 chain), EF1zz (ATLQLQEGRLHFXFDLGKGR, X = Nle; from laminin alpha1 chain), and 531 (GEFYFDLRLKGDKY; from collagen alpha1 (IV) chain) conjugated chitosan membranes promoted integrin-dependent cell adhesion. Various integrins, including alphav, beta1, and beta3, were involved in the cell adhesion to the peptide-chitosan membranes. Further, only the FIB1- and A99-chitosan membranes promoted neurite outgrowth with PC12 rat pheochromocytoma cells. These data demonstrate that peptide-chitosan membranes can regulate specific integrin-mediated cell responses and are useful constructs as ECM mimetics.
Mochizuki
Mayumi
M
Laboratory of Clinical Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan.
Yamagata
Natsumi
N
Philp
Deborah
D
Hozumi
Kentaro
K
Watanabe
Tadashi
T
Kikkawa
Yamato
Y
Kadoya
Yuichi
Y
Kleinman
Hynda K
HK
Nomizu
Motoyoshi
M
D000199:Actins / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry; D004492:Edetic Acid / Q000494:pharmacology; D016326:Extracellular Matrix Proteins / Q000737:chemistry*; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D016023:Integrins / Q000502:physiology*; D008567:Membranes, Artificial; D008969:Molecular Sequence Data; D016501:Neurites / Q000502:physiology / Q000648:ultrastructure; D016716:PC12 Cells; D010455:Peptides / Q000737:chemistry; D051381:Rats
D000199:Actins; D016326:Extracellular Matrix Proteins; D016023:Integrins; D008567:Membranes, Artificial; D010455:Peptides; D006493:Heparin; D048271:Chitosan; D004492:Edetic Acid