Fe(III)-enhanced Azo Reduction by Shewanella decolorationis S12.
Shewanella decolorationis S12 is capable of high rates of azo dye decolorization and dissimilatory Fe(III) reduction. Under anaerobic conditions, when Fe(III) and azo dye were copresent in S12 cultures, dissimilatory Fe(III) reduction and azo dye biodecolorization occurred simultaneously. Furthermore, the dye decolorization was enhanced by the presence of Fe(III). When 1 mM Fe(III) was added, the methyl red decolorizing efficiency was 72.1% after cultivation for 3 h, whereas the decolorizing efficiency was only 60.5% in Fe(III)-free medium. The decolorizing efficiencies increased as the concentration of Fe(III) was increased from 0 to 6 mM. Enzyme activities, which mediate the dye decolorization and Fe(III) reduction, were not affected by preadaption of cells to Fe(III) and azo dye nor by the addition of chloramphenicol. Both the Fe(III) reductase and the azo reductase were membrane associated. The respiratory electron transport chain inhibitors metyrapone, dicumarol, and stigmatellin showed significantly different effects on Fe(III) reduction than on azo dye decolorization.
Xu
Meiying
M
Guangdong Institute of Microbiology, 100 Central Xianlie Road, Guangzhou 510070, People's Republic of China.
Guo
Jun
J
Kong
Xiangyi
X
Chen
Xingjuan
X
Sun
Guoping
G
D000332:Aerobiosis; D000693:Anaerobiosis; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001391:Azo Compounds / Q000378:metabolism*; D002701:Chloramphenicol / Q000494:pharmacology; D002712:Chlorides; D004396:Coloring Agents / Q000378:metabolism; D005290:Ferric Compounds / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D020592:Shewanella / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors
D000900:Anti-Bacterial Agents; D001391:Azo Compounds; D002712:Chlorides; D004396:Coloring Agents; D005290:Ferric Compounds; C025314:ferric citrate; D002701:Chloramphenicol; C024555:ferric chloride