title: The C. elegans RSA complex localizes protein phosphatase 2A to centrosomes and regulates mitotic spindle assembly.
abstract: Microtubule behavior changes during the cell cycle and during spindle assembly. However, it remains unclear how these changes are regulated and coordinated. We describe a complex that targets the Protein Phosphatase 2A holoenzyme (PP2A) to centrosomes in C. elegans embryos. This complex includes Regulator of Spindle Assembly 1 (RSA-1), a targeting subunit for PP2A, and RSA-2, a protein that binds and recruits RSA-1 to centrosomes. In contrast to the multiple functions of the PP2A catalytic subunit, RSA-1 and RSA-2 are specifically required for microtubule outgrowth from centrosomes and for spindle assembly. The centrosomally localized RSA-PP2A complex mediates these functions in part by regulating two critical mitotic effectors: the microtubule destabilizer KLP-7 and the C. elegans regulator of spindle assembly TPXL-1. By regulating a subset of PP2A functions at the centrosome, the RSA complex could therefore provide a means of coordinating microtubule outgrowth from centrosomes and kinetochore microtubule stability during mitotic spindle assembly.
authors.affiliation: Max-Planck-Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.
authors.forename: Anne-Lore
authors.initials: AL
authors.lastname: Schlaitz
authors.forename: Martin
authors.initials: M
authors.lastname: Srayko
authors.forename: Alexander
authors.initials: A
authors.lastname: Dammermann
authors.forename: Sophie
authors.initials: S
authors.lastname: Quintin
authors.forename: Natalie
authors.initials: N
authors.lastname: Wielsch
authors.forename: Ian
authors.initials: I
authors.lastname: MacLeod
authors.forename: Quentin
authors.initials: Q
authors.lastname: de Robillard
authors.forename: Andrea
authors.initials: A
authors.lastname: Zinke
authors.forename: John R
authors.initials: JR
authors.lastname: Yates
authors.forename: Thomas
authors.initials: T
authors.lastname: Müller-Reichert
authors.forename: Andrei
authors.initials: A
authors.lastname: Shevchenko
authors.forename: Karen
authors.initials: K
authors.lastname: Oegema
authors.forename: Anthony A
authors.initials: AA
authors.lastname: Hyman
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D002384:Catalysis; D018385:Centrosome / Q000378:metabolism*; D019281:Dimerization; D004625:Embryo, Nonmammalian / Q000002:abnormalities / Q000166:cytology / Q000648:ultrastructure; D016547:Kinesins / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism; D046912:Multiprotein Complexes / Q000378:metabolism*; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D011485:Protein Binding; D054648:Protein Phosphatase 2; D021122:Protein Subunits / Q000378:metabolism; D021381:Protein Transport; D008941:Spindle Apparatus / Q000378:metabolism*
chemical_list: D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D046912:Multiprotein Complexes; D021122:Protein Subunits; C504801:TPXL-1 protein, C elegans; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2; D016547:Kinesins