[In vivo mutagenicity of the carcinogenic 1-nitropyrene. A model of a potential asbestos exposure].
The environmental carcinogen 1-nitropyrene was orally and intraperitoneally administered to rats in a single dose of 30 mmol/kg. Mutagenicity of excreted urine was tested in Salmonella typhimurium TA 98 and 100 strains. The mutagenic pattern of urine in case of oral exposure proved to be completely different as compared to the intraperitoneal administration. Frame-shift mutagen(s) was/were detected only after enzymatic deconjugation of sulphate or glucuronide metabolites within the first 24 h. Base-pair substitution-type mutagenicity was only detected in the urine samples collected after intraperitoneal treatment. Since environmental asbestos exposure involves carcinogenic effects of adsorbed polycyclic aromatic hydrocarbons, this animal model provides a useful tool for testing fiber-associated nitroarenes, in both mechanistic and risk assessment studies.
Varga
Csaba
C
Orvosi Népegészségtani Intézet, Környezet-egészségtani Tanszék, Pécsi Tudományegyetem, AOK, Pécs 7643, Hungary. chemsafety@freemail.hu
Szendi
Katalin
K
D000284:Administration, Oral; D000818:Animals; D001194:Asbestos / Q000009:adverse effects*; D020137:Base Pair Mismatch; D002273:Carcinogens / Q000008:administration & dosage*; D004195:Disease Models, Animal; D005260:Female; D016368:Frameshift Mutation; D007274:Injections, Intraperitoneal; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000008:administration & dosage* / Q000009:adverse effects*; D011721:Pyrenes / Q000008:administration & dosage* / Q000009:adverse effects*; D051381:Rats; D011916:Rats, Inbred F344; D012486:Salmonella typhimurium
D002273:Carcinogens; D009153:Mutagens; D011721:Pyrenes; D001194:Asbestos; C032668:1-nitropyrene