Selective expression of Bhlhb5 in subsets of early-born interneurons and late-born association neurons in the spinal cord.
Accumulating evidence has suggested that the basic helix-loop-helix transcription factors play important roles in controlling neuronal fate specification and differentiation in the developing central nervous system. In this study, we report a detailed immunological study on the expression of Bhlhb5 in embryonic mouse spinal cord with a newly developed antibody. At the early stage of neural development, Bhlhb5 is specifically expressed in dI6 dorsal interneurons and in V1 and V2 ventral interneurons. At late stages of development, Bhlhb5 expression is detected in a subset of late-born dorsal association interneurons that migrate into the uppermost layer of the dorsal horn.
Liu
Ben
B
The National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Liu
Zijing
Z
Chen
Tao
T
Li
Hong
H
Qiang
Boqin
B
Yuan
Jiangang
J
Peng
Xiaozhong
X
Qiu
Mengsheng
M
D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D002460:Cell Line; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D015151:Immunoblotting; D007395:Interneurons / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D011247:Pregnancy; D013116:Spinal Cord / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D014162:Transfection
D051792:Basic Helix-Loop-Helix Proteins; C488357:Bhlhe22 protein, mouse