title: Killing of feline T-lymphocytes by gamma-rays and energetic carbon ions.
abstract: High linear energy transfer (LET) heavy charged particles have previously been applied clinically to human cancer radiotherapy because of their excellent physical properties of selective dose distribution and higher relative biological effectiveness (RBE) for human; however, such an approach has yet to be applied to cat patients. The present study investigates the biological effectiveness of low-LET gamma-rays (0.2 keV/micro m) compared to high-LET carbon ions (114 keV/micro m) in feline T- lymphocyte FeT-J cells. Clonogenic survival analysis revealed that the RBE value of carbon ions was 2.98 relative to a 10% survival dose (D(10)) by gamma-rays, and that the inactivation cross-section in cells exposed to gamma-rays and carbon ions was 0.023 and 38.9 micro m(2), respectively. Terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end labeling (TUNEL) analysis revealed that TUNEL-positive frequency in carbon-irradiation cells is higher than for gamma-irradiated cells against exposure to the same physical doses, but that very little difference in TUNEL-positive frequency is observed between cells exposed to the respective D(10) dose of gamma-rays. Our data thus indicate that carbon ions are more effective for cell killing than gamma-rays at the same physical doses, but kill cells to an extent that is comparable to gamma-rays at the same biological doses. Carbon ion radiotherapy is therefore a promising modality for cat patients.
authors.affiliation: Department of Veterinary Medicine, Kitasato University Graduate School of Veterinary Medicine and Animal Sciences, Towada, Aomori, Japan.
authors.forename: Takehiko
authors.initials: T
authors.lastname: Kakizaki
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Hamada
authors.forename: Tomoo
authors.initials: T
authors.lastname: Funayama
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Sakashita
authors.forename: Seiichi
authors.initials: S
authors.lastname: Wada
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Hohdatsu
authors.forename: Masahiro
authors.initials: M
authors.lastname: Natsuhori
authors.forename: Tadashi
authors.initials: T
authors.lastname: Sano
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Ito
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002244:Carbon / Q000494:pharmacology*; D002415:Cats; D002478:Cells, Cultured; D005720:Gamma Rays; D020450:Heavy Ions / Q000009:adverse effects*; D013601:T-Lymphocytes / Q000166:cytology* / Q000528:radiation effects*
chemical_list: D002244:Carbon