Ethylene responsive element binding protein 1 (StEREBP1) from Solanum tuberosum increases tolerance to abiotic stress in transgenic potato plants.
To identify components of the plant stress signal transduction cascade and response mechanisms, we screened plant genes using reverse Northern blot analysis, and chose the ethylene responsive element binding protein 1 (StEREBP1) for further characterization. To investigate its biological function in the potato, we performed Northern blot analysis and observed enhanced levels of transcription in response to several environmental stresses including low temperature. In vivo targeting experiments using a green fluorescent protein (GFP) reporter indicated that StEREBP1 localized to the nucleus of onion epidermal cells. StEREBP1 was found to bind to GCC and DRE/CRT cis-elements and both microarray and RT-PCR analyses indicated that overexpression of StEREBP1 induced expression of several GCC box-containing stress response genes. In addition, overexpression of StEREBP1 enhanced tolerance to cold and salt stress in transgenic potato plants. The results of this study suggest that StEREBP1 is a functional transcription factor that may be involved in abiotic stress responses in plants.
Lee
Hye Eun
HE
Postharvest Technology Division, National Horticulture Research Institute, RDA, Suwon 440-706, Republic of Korea.
Shin
Dongjin
D
Park
Sang Ryeol
SR
Han
Sang-Eun
SE
Jeong
Mi-Jeong
MJ
Kwon
Tack-Ryun
TR
Lee
Seong-Kon
SK
Park
Soo-Chul
SC
Yi
Bu Young
BY
Kwon
Hawk-Bin
HB
Byun
Myung-Ok
MO
D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D015152:Blotting, Northern; D002467:Cell Nucleus / Q000378:metabolism; D003080:Cold Temperature; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D009838:Oligodeoxyribonucleotides / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011485:Protein Binding; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012965:Sodium Chloride / Q000494:pharmacology; D011198:Solanum tuberosum / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
D004268:DNA-Binding Proteins; D009838:Oligodeoxyribonucleotides; D010940:Plant Proteins; D018749:RNA, Plant; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; C093680:ethylene-responsive element binding protein; D012965:Sodium Chloride