title: Advanced glycation end products induce actin rearrangement and subsequent hyperpermeability of endothelial cells.
abstract: This study aimed to determine the effects of advanced glycation end products (AGEs) on endothelial cytoskeleton morphology and permeability, and to detect the underlying signaling mechanisms involved in these responses. Cultured endothelial cells (ECs) were exposed to AGE-modified human serum albumin (AGE-HSA), and EC cytoskeletal changes were evaluated by observing fluorescence of F-actin following ligation with labeled antibodies. Endothelial permeability was detected by measuring the flux of TRITC-albumin across the EC monolayers. To explore the signaling pathways behind AGE-induced EC alteration, ECs were treated with either soluble anti-AGE receptor (RAGE) IgG, or the MAPK inhibitors PD98059 and SB203580 before AGE-HSA administration. To further elucidate possible involvement of the ERK and p38 pathways in AGE-induced EC changes, adenovirus-carried recombinant constitutive dominant-negative forms of upstream ERK and p38 kinases, namely MEK1(A) and MKK6b(A), were pre-infected into ECs 24 h prior to AGE-HSA exposure. AGE-HSA induced actin cytoskeleton rearrangement, as well as EC hyperpermeability, in a dose and time-dependent manner. The effects were attenuated in cells pretreated with anti-RAGE IgG, PD98059 or SB203580, respectively. EC pre-infection with MEK1(A) and MKK6b(A) also alleviated the effect of AGEs. Furthermore, adenovirus-mediated administration of activated forms of either MEK1 or MKK6b alone induced rearrangement of F-actin and hyperpermeability. The results indicate that ERK and p38 MAPK play important roles in the mediation of AGE-induced EC barrier dysfunction associated with morphological changes of the F-actin.
authors.affiliation: Department of Pathophysiology, Southern Medical University, Guangzhou, PR China.
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Guo
authors.forename: Qiao-Bing
authors.initials: QB
authors.lastname: Huang
authors.forename: Bo
authors.initials: B
authors.lastname: Chen
authors.forename: Shu-Yun
authors.initials: SY
authors.lastname: Wang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Zhu
authors.forename: Fan-Fan
authors.initials: FF
authors.lastname: Hou
authors.forename: Ning
authors.initials: N
authors.lastname: Fu
authors.forename: Ulf T
authors.initials: UT
authors.lastname: Brunk
authors.forename: Ming
authors.initials: M
authors.lastname: Zhao
mesh_terms: D000199:Actins / Q000378:metabolism*; D002199:Capillary Permeability / Q000187:drug effects; D002460:Cell Line; D003599:Cytoskeleton / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology; D017127:Glycation End Products, Advanced / Q000494:pharmacology*; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008856:Microscopy, Fluorescence; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000276:immunology; D012709:Serum Albumin / Q000494:pharmacology*; D000075462:Serum Albumin, Human; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D000199:Actins; D005419:Flavonoids; D017127:Glycation End Products, Advanced; D007093:Imidazoles; D047428:Protein Kinase Inhibitors; D011725:Pyridines; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic; D012709:Serum Albumin; C109748:advanced glycation end products-human serum albumin; D048049:Extracellular Signal-Regulated MAP Kinases; D048051:p38 Mitogen-Activated Protein Kinases; C093642:SB 203580; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one; D000075462:Serum Albumin, Human