title: Direct modification of mRNA by ferrocenyl carbodiimide and its application to electrochemical detection of mRNA.
abstract: Ferrocenyl carbodiimide (1) could be used for the direct labeling of synthetic RNA and expressed mRNA in vitro with the electrochemically active ferrocene moieties. These RNAs modified by 1 could be detected electrochemically coupled with a DNA probe-immobilized electrode. After hybridization of 1.1 Kb mRNA modified by 1 with the DNA probe-immobilized electrode, the peak charge observed by an Osteryoung square wave voltammetry (SWV) measurement correlated well with the concentration of mRNA, having a detection limit at the sub nanogram level.
authors.affiliation: Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Mukumoto
authors.forename: Takahiko
authors.initials: T
authors.lastname: Nojima
authors.forename: Shinobu
authors.initials: S
authors.lastname: Sato
authors.forename: Michinori
authors.initials: M
authors.lastname: Waki
authors.forename: Shigeori
authors.initials: S
authors.lastname: Takenaka
mesh_terms: D002234:Carbodiimides / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D015342:DNA Probes; D018076:DNA, Complementary / Q000737:chemistry; D004563:Electrochemistry; D005296:Ferrous Compounds / Q000737:chemistry*; D007202:Indicators and Reagents; D012333:RNA, Messenger / Q000032:analysis* / Q000138:chemical synthesis / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D002234:Carbodiimides; D015342:DNA Probes; D018076:DNA, Complementary; D005296:Ferrous Compounds; D007202:Indicators and Reagents; D012333:RNA, Messenger; C517870:ferrocenyl carbodiimide