Procyanidin Production by Fagopyrum esculentum Callus Culture.
FAGOPYRUM ESCULENTUM CALLUS cultures grown on B5 medium synthesize procyanidins B2 (0.6-1.8 mg/g dry wt) and B2-3'- O-gallate (3.5-6.0 mg/g dry wt). Sucrose is a better source than other carbohydrates and moderate concentrations (3-4%) stimulate both growth and procyanidin synthesis. While darkness was not a limiting factor, light induced faster growth and increased procyanidin contents. Treatment with gallic acid strongly stimulated the procyanidin B2-3'- O-gallate production, but drastically inhibited growth, leading to a two-step-culture experiment combining convenient growth and increased galloylated dimer synthesis (up to 45 mg/g dry wt).
Moumou
Y
Y
Laboratoire de Physiologie et Morphogenése Végétales, Université des Sciences et Techniques de Lille-Flandres Artois, F-59655 Villeneuve d'Ascq Cedex, France.
Trotin
F
F
Vasseur
J
J
Vermeersch
G
G
Guyon
R
R
Dubois
J
J
Pinkas
M
M