Rare carotenoids, (3R)-saproxanthin and (3R,2'S)-myxol, isolated from novel marine bacteria (Flavobacteriaceae) and their antioxidative activities.
We isolated three orange or yellow pigment-producing marine bacteria, strains 04OKA-13-27 (MBIC08261), 04OKA-17-12 (MBIC08260), and YM6-073 (MBIC06409), off the coast of Okinawa Prefecture in Japan. These strains were classified as novel species of the family Flavobacteriaceae based on their 16S rRNA gene sequence. They were cultured, and the major carotenoids produced were purified by chromatographic methods. Their structures were determined by spectral data to be (3R)-saproxanthin (strain 04OKA-13-27), (3R,2'S)-myxol (strain YM6-073), and (3R,3'R)-zeaxanthin (strains YM6-073 and 04OKA-17-12). Saproxanthin and myxol, which are monocyclic carotenoids rarely found in nature, demonstrated significant antioxidative activities against lipid peroxidation in the rat brain homogenate model and a neuro-protective effect from L-glutamate toxicity.
Shindo
Kazutoshi
K
Department of Food and Nutrition, Japan Women's University, 2-8-1 Mejirodai, Tokyo 112-8681, Japan. Kshindo@fc.jwu.ac.jp
Kikuta
Kana
K
Suzuki
Atsuko
A
Katsuta
Atsuko
A
Kasai
Hiroaki
H
Yasumoto-Hirose
Mina
M
Matsuo
Yoshihide
Y
Misawa
Norihiko
N
Takaichi
Shinichi
S
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002338:Carotenoids / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D041965:Flavobacteriaceae / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D018698:Glutamic Acid / Q000633:toxicity; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D051381:Rats; D011916:Rats, Inbred F344; D017208:Rats, Wistar; D017422:Sequence Analysis, DNA; D024341:Xanthophylls / Q000737:chemistry / Q000494:pharmacology*; D065146:Zeaxanthins
D000975:Antioxidants; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D024341:Xanthophylls; D065146:Zeaxanthins; C488895:myxol; D002338:Carotenoids; D018698:Glutamic Acid