Cell cycle progression and de novo centriole assembly after centrosomal removal in untransformed human cells.
How centrosome removal or perturbations of centrosomal proteins leads to G1 arrest in untransformed mammalian cells has been a mystery. We use microsurgery and laser ablation to remove the centrosome from two types of normal human cells. First, we find that the cells assemble centrioles de novo after centrosome removal; thus, this phenomenon is not restricted to transformed cells. Second, normal cells can progress through G1 in its entirety without centrioles. Therefore, the centrosome is not a necessary, integral part of the mechanisms that drive the cell cycle through G1 into S phase. Third, we provide evidence that centrosome loss is, functionally, a stress that can act additively with other stresses to arrest cells in G1 in a p38-dependent fashion.
Uetake
Yumi
Y
Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01605, USA.
Loncarek
Jadranka
J
Nordberg
Joshua J
JJ
English
Christopher N
CN
La Terra
Sabrina
S
Khodjakov
Alexey
A
Sluder
Greenfield
G
D001973:Bromodeoxyuridine / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000032:analysis; D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D002478:Cells, Cultured; D002502:Centrioles / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D018385:Centrosome / Q000502:physiology*; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D016193:G1 Phase / Q000502:physiology; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D008027:Light; D008854:Microscopy, Electron; D011725:Pyridines / Q000494:pharmacology; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism
D002135:Calcium-Binding Proteins; D002868:Chromosomal Proteins, Non-Histone; D004791:Enzyme Inhibitors; D007093:Imidazoles; D011725:Pyridines; C056608:caltractin; D048051:p38 Mitogen-Activated Protein Kinases; D001973:Bromodeoxyuridine; C093642:SB 203580