Anticlastogenic activity of morin against whole body gamma irradiation in Swiss albino mice.
Anticlastogenic activity of morin was explored against whole body gamma radiation, at a dose rate of 1.66 Gy/min in Swiss albino mice pretreated intraperitoneal or orally. Pretreatment with morin 10, 25, 50, 75, 100, 125, and 150 mg/kg, i.p. delayed and reduced percentage mortality and increased mean survival times in mice irradiated with 10 Gy gamma radiation. Intraperitoneal route was found superior to oral route. An i.p. dose of 100 mg/kg was found to be the most effective dose in preventing radiation-induced weight loss, increasing the mean survival times and reducing percentage mortality. Morin (100 mg/kg) pretreatment effectively maintained spleen index (spleen weight/body weight x 100) and stimulated endogenous spleen colony forming units. Pretreatment with morin (100 mg/kg) significantly reduced dead, inflammatory, and mitotic cells in irradiated mice jejunum along with a significant increase in goblet cells and rapidly multiplying crypt cells. Morin (100 mg/kg) also maintained the villus height close to normal, prevented mucosal erosion and basement membrane damage in irradiated jejunum. Nuclear enlargement in epithelial cells of jejunum was lower in morin treated mice compared to radiation control. Morin (100 mg/kg) also significantly elevated the endogenous antioxidant enzymes viz. glutathione S transferase (GST), superoxide dismutase (SOD) and reduced glutathione (GSH), in normal mice at 2, 4 and 8 h post treatment. Drastic decrease in endogenous enzymes (GSH, GST, catalase and SOD) and total thiols was observed in irradiated mice at 2, 4 and 8 h post irradiation, while pretreatment with morin (100 mg/kg) prevented this decrease. Morin (100 mg/kg) also elevated radiation LD(50) from 9.2 to 10.1 Gy, indicating a dose modifying factor (DMF) of 1.11.
Parihar
Vipan Kumar
VK
Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal-576104, Karnataka, India.
Prabhakar
Koiram Rajanna
KR
Veerapur
Veeresh Prabhakar
VP
Priyadarsini
Kavirayani Indira
KI
Unnikrishnan
Mazhuvancherry Kesavan
MK
Rao
Chamallamudi Mallikajuna
CM
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology*; D005720:Gamma Rays; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D007583:Jejunum / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D007928:Lethal Dose 50; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013154:Spleen / Q000187:drug effects / Q000254:growth & development / Q000528:radiation effects; D013482:Superoxide Dismutase / Q000378:metabolism; D014916:Whole-Body Irradiation; D047309:Flavones
D000975:Antioxidants; D002374:Catalase; D005419:Flavonoids; D005978:Glutathione; D005982:Glutathione Transferase; D008315:Malondialdehyde; D011837:Radiation-Protective Agents; D013482:Superoxide Dismutase; C008548:morin; D047309:Flavones