title: Identification of specific protein markers in microdissected hepatocellular carcinoma.
abstract: At present, the molecular mechanisms of hepatocellular carcinogenesis are not well-understood, and hepatocellular carcinoma (HCC) stays one of the most frequent and high-risk metastatic visceral neoplasms worldwide. For the identification of tumor-relevant proteins, we analyzed microdissected cells from nontumorous liver tissue (n = 28) and tissue derived from hepatic tumor center (n = 25), as well as tumor margin (n = 23). We unequivocally identified 53 proteins from hepatic tumor tissues by peptide fingerprint mapping and SELDI mass spectrometry that were separated using two-dimensional gel electrophoresis. Among a number of signals that were detected as significantly different in the protein profiling analysis, we identified for the first time ferritin light subunit (FLS) and adenylate kinase 3 alpha-like 1 (AK3), showing decreased expressions in hepatic tumor, as well as biliverdin reductase B (BVRB) that was upregulated in HCC. The use of ProteinChip technology in combination with tissue microdissection gives insight of the complex changes occurring at the protein level in hepatocellular cancer associated with tumor development and progression and resulted in three new potential diagnostically useful markers.
authors.affiliation: Core Unit Chip Application (CUCA), Institute of Human Genetics and Anthropology, Medical Faculty at the Friedrich Schiller University, 07740 Jena, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Melle
authors.forename: Günther
authors.initials: G
authors.lastname: Ernst
authors.forename: Olaf
authors.initials: O
authors.lastname: Scheibner
authors.forename: Roland
authors.initials: R
authors.lastname: Kaufmann
authors.forename: Bettina
authors.initials: B
authors.lastname: Schimmel
authors.forename: Annett
authors.initials: A
authors.lastname: Bleul
authors.forename: Utz
authors.initials: U
authors.lastname: Settmacher
authors.forename: Merten
authors.initials: M
authors.lastname: Hommann
authors.forename: Uwe
authors.initials: U
authors.lastname: Claussen
authors.forename: Ferdinand
authors.initials: F
authors.lastname: von Eggeling
mesh_terms: D015415:Biomarkers; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D007834:Lasers; D008099:Liver / Q000378:metabolism*; D008113:Liver Neoplasms / Q000378:metabolism*; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000378:metabolism*; D011379:Prognosis; D040081:Protein Array Analysis; D040901:Proteomics / Q000379:methods*
chemical_list: D015415:Biomarkers; D009363:Neoplasm Proteins