[Study on therapy of experimental autoimmune neuritis by bone marrow stromal cells].
OBJECTIVE
To investigate the therapeutic effect of bone marrow stromal cells (BMSCs) transplantation on experimental autoimmune neuritis (EAN) and study the possible mechanism.


METHODS
EAN model was established by immunizing Lewis rats with P(0)180-199 peptide and complete Freund's adjuvant (CFA). In the therapy group, BMSCs (2x10(6) cells/rat) were marked with fluorochrome PKH26 and injected into the rats' caudal vein 10 d after immunization. The therapeutic effect of BMSC transplantation on EAN rats was investigated by clinical assessment, immunohistochemical staining, and ELISA, respectively.


RESULTS
The injected BMSCs could migrate to the demyelinated nerve tissues, and the demyelination and inflammatory infiltration was relieved. The infiltration of CD4(+) and CD8(+) T cells and the sera level of IFN-gamma and TNF-alpha were decreased significantly (P<0.05), whereas IL-4 level in the supernatant of cultured lymphocytes was increased (P<0.05).


CONCLUSIONS
Certain therapeutic effect of BMSC transplantation on EAN was observed. BMSCs could reverse the disbalance of Th1/Th2 cells by regulating the cytokine expression and could inhibit the activation and proliferation of T cells.
Xu
Xin
X
Department of Neurobiology, Harbin Medical University, Key Laboratory of Heilongjiang Province, Harbin 150086, China. xinxu81@yahoo.com.cn
Sun
Bo
B
Mu
Li-li
LL
Wang
Guang-you
GY
Jin
Lian-hong
LH
Li
Hu-lun
HL
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D016026:Bone Marrow Transplantation / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D007150:Immunohistochemistry; D009444:Neuritis, Autoimmune, Experimental / Q000473:pathology / Q000628:therapy*; D011897:Random Allocation; D051381:Rats; D012584:Sciatic Nerve / Q000473:pathology; D020426:Sciatic Neuropathy / Q000473:pathology / Q000628:therapy; D017154:Stromal Cells / Q000166:cytology*