[Spectroscopic studies on the binding of prulifloxacin and ct-DNA].
The interaction mechanism of prulifloxacin (PL) and calf thymus DNA (ct-DNA) was studied by UV spectra, fluorescence spectra, and hydrodynamic measurements. The binding of ct-DNA and different concentrations of PL was discussed with UV, FL, phosphate effect and ion strength. The denaturation temperature and viscosity were measured. It is obvious that there is a hypochromic effect on the UV spectra with the fluorescence intensity decreasing regularly after addition of DNA and a red shift of the maximum emission peak with a static quenching constant of 3.1 x 10(4) L x mol(-1). The above results show that a binary complex forms between PL and DNA. It is indicated that there is no static-electro interaction between them by phosphate effect. The outer groove binding is suggested by denaturation temperature of DNA increasing (no more than 7 degrees C) and viscosity slightly decreasing.
Chen
Chang-yun
CY
Department of Chemistry, Nanjing Xiaozhuang College, Nanjing 210017, China.
Ma
Mei-hua
MH
Zhou
Zhi-hua
ZH
Lu
Kang
K
Xiang
Bing-ren
BR
D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D004148:Dioxolanes / Q000737:chemistry*; D004347:Drug Interactions; D024841:Fluoroquinolones / Q000737:chemistry*; D010879:Piperazines / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
D004148:Dioxolanes; D024841:Fluoroquinolones; D010879:Piperazines; D004247:DNA; C427203:calf thymus DNA; C074191:prulifloxacin