title: Serum protease inhibitor concentrations and total antitrypsin activity in diabetic and non-diabetic children during adolescence.
abstract: The aim of the study was the assessment of the concentrations and establishment of mutual relationships between three main protease inhibitors: alpha-1-antitrypsin (AAT), alpha-2-macroglobulin (alpha-2-M) and antithrombin-III (AT-III), and of the total trypsin inhibitory capacity (TIC) in the serum of diabetic and non-diabetic children during adolescence. Forty-nine children (24 girls and 25 boys) with type 1 diabetes mellitus and 24 non-diabetic children (13 girls and 11 boys) were divided according to the Tanner scale into three groups: pre-, peri- and post-pubertal. The concentrations of AAT, alpha-2-M and AT-III were determined by the radial immunodiffusion method on NOR-Partigen plates (Dade-Behring), while TIC was determined by the method using BAPNA as substrate. Means and medians of serum AAT [1.55 g/l, 1.40 (95% confidence interval, 1.42-1.68), respectively] and TIC [10.6 mg trypsin/100 ml, 10.3 (95% CI, 9.5-11.7)] in diabetic children were lower than means and medians of AAT [1.81 g/l, 1.60 (95% CI 1.55-2.07), respectively] and TIC [12.5 mg trypsin/100 ml, 13.2 (95% CI, 10.9-14.1)] in non-diabetic children. A comparison of variables between Tanner groups shows an increasing trend of AAT concentration in diabetic children and a decreasing trend of TIC in non-diabetic subjects. In contrast to pre- and peri-puberty, no correlations were found in the postpubertal period between the studied parameters, either in diabetic or non-diabetic patients. Hyperglycaemia and the duration of diabetes were found to have a significant association with alpha-2-M and AT-III concentrations, but not with AAT serum concentrations. The concentrations and correlations between serum protease inhibitors in diabetic children during adolescence are disrupted compared with non-diabetic children. Taking into account the unfavourable consequences of vascular complications resulting from serum trypsin inhibitor changes and protease- antiprotease imbalance, diabetic children are at greater risk of this occurring during adolescence.
authors.affiliation: Department of Biochemistry and Clinical Chemistry, Medical University in Warsaw, ul. Banacha 1, 02-097, Warsaw, Poland. tomasz@artibus.pl
authors.forename: B
authors.initials: B
authors.lastname: Lisowska-Myjak
authors.forename: J
authors.initials: J
authors.lastname: Pachecka
authors.forename: B
authors.initials: B
authors.lastname: Kaczyńska
authors.forename: G
authors.initials: G
authors.lastname: Miszkurka
authors.forename: K
authors.initials: K
authors.lastname: Kadziela
mesh_terms: D000293:Adolescent; D000990:Antithrombin III / Q000378:metabolism; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D005779:Immunodiffusion; D008297:Male; D011480:Protease Inhibitors / Q000097:blood*; D012016:Reference Values; D000515:alpha 1-Antitrypsin / Q000097:blood*
chemical_list: D000990:Antithrombin III; D006442:Glycated Hemoglobin; D011480:Protease Inhibitors; D000515:alpha 1-Antitrypsin