Identification of differentially expressed genes in lipopolysaccharide-stimulated yellow grouper Epinephelus awoara spleen.
A subtracted cDNA library from lipopolysaccharide (LPS)-stimulated Epinephelus awoara spleen was constructed using the suppression subtractive hybridization (SSH). Random clones (209) were selected and sequenced. After assembling, 36 contigs and 56 singlets (accession numbers: EB410743-EB410834) were finally obtained, some of which were immune-related genes. A reverse transcription-polymerase chain reaction (RT-PCR) analysis of the expression patterns of eight transcripts showed that seven of them were up-regulated after 24 h of LPS stimulation. Furthermore, full-length cDNAs of homologues of defender against cell death 1 (DAD1) and allograft inflammatory factor-1 (AIF-1) were obtained by RACE-PCR.
Wang
Li
L
Laboratory of Marine Life Science and Technology, College of Animal Sciences, Zhejiang University, 268 Kaixuan Road, Hangzhou, Zhejiang 310029, China.
Wu
Xinzhong
X
D000595:Amino Acid Sequence; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D001492:Bass / Q000235:genetics* / Q000276:immunology*; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology* / Q000494:pharmacology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013154:Spleen / Q000276:immunology / Q000502:physiology*
D051017:Apoptosis Regulatory Proteins; D004268:DNA-Binding Proteins; D008070:Lipopolysaccharides; D012333:RNA, Messenger