Overexpression of citrus polygalacturonase-inhibiting protein in citrus black rot pathogen Alternaria citri.
The rough lemon (Citrus jambhiri) gene encoding polygalacturonase-inhibiting protein (RlemPGIPA) was overexpressed in the pathogenic fungus Alternaria citri. The overexpression mutant AcOPI6 retained the ability to utilize pectin as a sole carbon source, and the overexpression of polygalacturonase-inhibiting protein did not have any effect on the growth of AcOPI6 in potato dextrose and pectin medium. The pathogenicity of AcOPI6 to cause a black rot symptom in citrus fruits was also unchanged. Polygalacturonase-inhibiting protein was secreted together with endopolygalacturonase into culture filtrates of AcOPI6, and oligogalacturonides were digested from polygalacturonic acid by both proteins in the culture filtrates. The reaction mixture containing oligogalacturonides possessed activity for induction of defense-related gene, RlemLOX, in rough lemon leaves.
Katoh
Hiroshi
H
Laboratory of Plant Pathology, United Graduate School and Faculty of Agriculture, Kagawa University, Miki, Kagawa 761-0795 Japan.
Nalumpang
Sarunya
S
Yamamoto
Hiroyuki
H
Akimitsu
Kazuya
K
D000528:Alternaria / Q000378:metabolism*; D002957:Citrus / Q000201:enzymology* / Q000382:microbiology*; D005638:Fruit / Q000382:microbiology; D005656:Fungal Proteins / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D018506:Gene Expression Regulation, Plant; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000378:metabolism; D011096:Polygalacturonase / Q000037:antagonists & inhibitors*
D005656:Fungal Proteins; D011096:Polygalacturonase