Oxidative stress involvement and gene expression in indomethacin-induced gastropathy.
It has been proposed that neutrophil- and oxygen radical-dependent microvascular injuries are important prime events that lead to gastric mucosal injury induced by indomethacin. Reactive oxygen species (ROS) produced by activated neutrophils after indomethacin treatment cause gastric mucosal injury via ROS-mediated oxidation of important biomolecules such as lipid, protein, and DNA. In addition, it has been revealed that indomethacin-induced gastric mucosal injury occurs via gastric epithelial cell apoptosis. However, there is little known about the mechanism of indomethacin-triggered cellular response and apoptotic signaling in gastric mucosal cells. In the present study, we summarize the evidence that supports the involvement of oxidative stress and apoptosis in indomethacin-induced gastropathy, and review the gene expression profiles of gastric epithelial cells after indomethacin treatment determined by DNA microarray analysis.
Naito
Yuji
Y
Department of Medical Proteomics, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Yoshikawa
Toshikazu
T
D000409:Alanine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology; D017209:Apoptosis; D004247:DNA / Q000378:metabolism; D005753:Gastric Mucosa / Q000473:pathology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress; D015363:Quinolones / Q000494:pharmacology; D051381:Rats; D017382:Reactive Oxygen Species; D013272:Stomach Diseases / Q000139:chemically induced*
D000897:Anti-Ulcer Agents; D015363:Quinolones; D017382:Reactive Oxygen Species; D004247:DNA; C052785:rebamipide; D000409:Alanine; D007213:Indomethacin