title: Suspension-cultured BY-2 tobacco cells produce and mature immunologically active house dust mite allergens.
abstract: The replacement of crude allergen extracts by selected allergens currently represents a major goal for the improvement of allergy diagnosis and immunotherapy. Indeed, the development of molecularly defined vaccines would facilitate both standardization and enhance batch-to-batch reproducibility as well as treatment specificity. In this study, we have investigated the potential of tobacco plant cells to produce biologically active forms of the two major allergens from the house dust mite. A detailed characterization of these plant-made allergens has shown similar proteolytic maturation and folding as well as comparable immunoreactivity to their natural counterparts. Altogether, our results exemplify that suspension-cultured BY-2 tobacco cells represent a low cost and environmentally safe expression system suitable to produce recombinant allergens from Dermatophagoides pteronyssinus under a form appropriate for diagnostic and therapeutic purposes.
authors.affiliation: CNRS UMR 6037, IFRMP 23, GDR 2590, Université de Rouen, 76821 Mont Saint Aignan, France.
authors.forename: David
authors.initials: D
authors.lastname: Lienard
authors.forename: Olivia
authors.initials: O
authors.lastname: Tran Dinh
authors.forename: Erika
authors.initials: E
authors.lastname: van Oort
authors.forename: Laurence
authors.initials: L
authors.lastname: Van Overtvelt
authors.forename: Caroline
authors.initials: C
authors.lastname: Bonneau
authors.forename: Eric
authors.initials: E
authors.lastname: Wambre
authors.forename: Muriel
authors.initials: M
authors.lastname: Bardor
authors.forename: Pascal
authors.initials: P
authors.lastname: Cosette
authors.forename: Alain
authors.initials: A
authors.lastname: Didier-Laurent
authors.forename: François Dorlhac
authors.initials: FD
authors.lastname: de Borne
authors.forename: Rene
authors.initials: R
authors.lastname: Delon
authors.forename: Ronald
authors.initials: R
authors.lastname: van Ree
authors.forename: Philippe
authors.initials: P
authors.lastname: Moingeon
authors.forename: Loïc
authors.initials: L
authors.lastname: Faye
authors.forename: Véronique
authors.initials: V
authors.lastname: Gomord
mesh_terms: D000485:Allergens / Q000235:genetics* / Q000276:immunology; D000818:Animals; D018929:Cell Culture Techniques; D030821:Plants, Genetically Modified / Q000166:cytology / Q000378:metabolism; D039981:Pyroglyphidae / Q000276:immunology*; D011994:Recombinant Proteins / Q000096:biosynthesis; D014026:Nicotiana / Q000166:cytology / Q000235:genetics*
chemical_list: D000485:Allergens; D011994:Recombinant Proteins