Heterologous production of albicidin: a promising approach to overproducing and characterizing this potent inhibitor of DNA gyrase.
The phytotoxin and polyketide antibiotic albicidin produced by Xanthomonas albilineans is a highly potent DNA gyrase inhibitor. Low yields of albicidin production have slowed studies of its chemical structure. Heterologous expression of albicidin biosynthetic genes in X. axonopodis pv. vesicatoria resulted in a sixfold increase in albicidin production, offering promising strategies for engineering overproduction.
Vivien
Eric
E
Centre de Coopération Internationale en Recherche Agronomique pour le Développement, UMR Agro.M-Cirad-Inra BGPI, Campus International de Baillarguet, TA 41/K, 34398 Montpellier Cedex 5, France.
Pitorre
Delphine
D
Cociancich
Stéphane
S
Pieretti
Isabelle
I
Gabriel
Dean W
DW
Rott
Philippe C
PC
Royer
Monique
M
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics*; D009930:Organic Chemicals / Q000378:metabolism / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics* / Q000378:metabolism; D059005:Topoisomerase II Inhibitors; D014974:Xanthomonas / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D009930:Organic Chemicals; D059005:Topoisomerase II Inhibitors; C046156:albicidin