Some remarks on the use of TaqI to detect highly repetitive DNA sequences in human chromosomes.
In the attempt to conclude investigation of the action of restriction endonucleases on eukaryote chromosomes, we carried out a series of experiments digesting in situ human metaphase chromosomes with AluI/TaqI followed by Giemsa staining. We focused on the centromeric regions of chromosomes1, 2 and 16 and noted that those areas appeared as intensely stained blocks after AluI digestion, but were dramatically reduced in size or completely destroyed after subsequent TaqI treatment. These results permitted us to draw some conclusions on the highly repetitive DNA composition of these regions, in terms of alphoid and classical satellite DNAs.
Nieddu
M
M
Sezione di Biologia e Genetica, Dipartimento di Scienze e Tecnologie Biomediche, Cittadella Universitaria 09042 Monserrato (CA), Italy.
Pichiri
G
G
Manconi
S
S
Mezzanotte
R
R
D001399:Azure Stains; D002877:Chromosomes, Human / Q000737:chemistry*; D004247:DNA / Q000032:analysis*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism; D008677:Metaphase; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D019914:Taq Polymerase
D001399:Azure Stains; D004247:DNA; D019914:Taq Polymerase; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific; C027046:TCGA-specific type II deoxyribonucleases