Hydrolytic activities of anaerobic fungi from wild blue bull (Boselaphus tragocamelus).
The anaerobic fungi play an active role in the plant fibre degradation by producing a wide array of potential hydrolytic enzymes in the rumen. In present study, 12 anaerobic fungal strains were isolated from the faecal samples of wild blue bull, and identified as species of Piromyces, Anaeromyces, Orpinomyces and Neocallimastix based on their morphological characteristics. Isolate WNG-12 (Piromyces sp.), showed maximum filter paper cellulase (23 mIU ml(-1)) and xylanase (127 mIU ml(-1)) activity, while WNG-5 (Piromyces sp.) showed maximum carboxymethyl cellulase activity (231 mIU ml(-1)). Based on the results obtained, it can be stated that Piromyces sp. WNG-12 may be a promising isolate in utilizing fibre rich diets in the rumen as evidenced by the production of hydrolytic enzymes in vitro.
Tripathi
Vimal Kumar
VK
Dairy Cattle Nutrition Division, National Dairy Research Institute, Karnal 132001, Haryana, India.
Sehgal
Jatinder Paul
JP
Puniya
Anil Kumar
AK
Singh
Kishan
K
D000693:Anaerobiosis; D000818:Animals; D000866:Antelopes / Q000382:microbiology*; D002417:Cattle; D002480:Cellulase / Q000378:metabolism; D005658:Fungi / Q000201:enzymology*; D006868:Hydrolysis; D008297:Male; D020089:Piromyces / Q000201:enzymology; D012417:Rumen / Q000382:microbiology*; D014995:Xylosidases / Q000378:metabolism
D014995:Xylosidases; D002480:Cellulase; C019826:carboxymethylcellulase