title: In vivo regulation of Homer1a expression in the striatum by cocaine.
abstract: The glutamate receptor adaptor protein Homer is concentrated in the postsynaptic density of excitatory synapses and is critical for normal operation of synaptic transmission. In this study, we investigated the responsiveness of Homer family proteins to dopamine stimulation with the psychostimulant cocaine in rat striatal neurons both in vivo and in vitro. We found that a single dose of cocaine specifically induced a rapid and transient increase in protein levels of the Homer1a, but not Homer1b/c and Homer2a/b, isoforms in the striatum. This selective Homer1a induction was mediated primarily through activation of dopamine D1, but not D2, receptors. Both protein kinase A and Ca(2+)/calmodulin-dependent protein kinases are important for mediating the cocaine stimulation of Homer1a expression. At the transcriptional level, cAMP response element-binding protein serves as a prime transcription factor transmitting the signals derived from D1 receptors and associative pathways to the CaCRE sites within the Homer1a promoter. From a functional perspective, non-cross-linking Homer1a, once induced, competed with the cross-linking isoforms of Homer proteins (Homer1b/c and Homer2a/b) to uncouple the connection of group I metabotropic glutamate receptors (mGluRs) with inositol-1,4,5-triphosphate receptors. These results indicate that cocaine possesses the ability to stimulate Homer1a expression in striatal neurons through a specific synapse-to-nucleus pathway. Moreover, inducible Homer1a expression may represent a transcription-dependent mechanism underlying the dynamic regulation of submembranous macromolecular complex formation between group I mGluRs and their anchoring proteins.
authors.affiliation: Department of Basic Medical Science, University of Missouri-Kansas City, School of Medicine, 2411 Holmes Street, Kansas City, MO 64108, USA.
authors.forename: Guo-Chi
authors.initials: GC
authors.lastname: Zhang
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Mao
authors.forename: Xian-Yu
authors.initials: XY
authors.lastname: Liu
authors.forename: Nikhil K
authors.initials: NK
authors.lastname: Parelkar
authors.forename: Anish
authors.initials: A
authors.lastname: Arora
authors.forename: Lu
authors.initials: L
authors.lastname: Yang
authors.forename: Michelle
authors.initials: M
authors.lastname: Hains
authors.forename: Eugene E
authors.initials: EE
authors.lastname: Fibuch
authors.forename: John Q
authors.initials: JQ
authors.lastname: Wang
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D003042:Cocaine / Q000494:pharmacology*; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects*; D000072233:Homer Scaffolding Proteins; D008297:Male; D046912:Multiprotein Complexes / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011954:Receptors, Dopamine / Q000502:physiology; D018094:Receptors, Metabotropic Glutamate / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D002352:Carrier Proteins; D000072233:Homer Scaffolding Proteins; D046912:Multiprotein Complexes; D011954:Receptors, Dopamine; D018094:Receptors, Metabotropic Glutamate; D003042:Cocaine