title: Rutin and Scopoletin Content and Micropropagation of Fabiana imbricata.
abstract: An HPLC method for the simultaneous quantitative determination of rutin and scopoletin in the aerial parts of FABIANA IMBRICATA is presented. The results showed high variability in the scopoletin (240-2,400 mg%) and rutin (195-1,950 mg%) content in the populations surveyed. A micropropagation method for F. IMBRICATA plants was established by culturing shoot tips on Murashige-Skoog (MS) medium containing 1.0 mg/l benzylaminopurine (BAP), 0.01 mg/l naphthaleneacetic acid (NAA), and 0.1 mg/l gibberellic acid (GA3). Auxin addition to the rooting medium, especially 0.5 mg/l indolebutyric acid (IBA) or 0.5 mg/l IBA and 0.1 mg/l NAA, enhances root formation. The micropropagation method presented allowed the obtention of regenerated plantlets in six weeks from shoot tips.
authors.affiliation: Departamento de Ciencias Biológicas, Universidad de Talca, Casilla 747, Talca, Chile.
authors.forename: I
authors.initials: I
authors.lastname: Razmilic
authors.forename: G
authors.initials: G
authors.lastname: Schmeda-Hirschmann
authors.forename: M
authors.initials: M
authors.lastname: Dutra-Behrens
authors.forename: S
authors.initials: S
authors.lastname: Reyes
authors.forename: I
authors.initials: I
authors.lastname: López
authors.forename: C
authors.initials: C
authors.lastname: Theoduloz