Proteomic profiling of a snake venom using high mass detection MALDI-TOF mass spectrometry.
Proteomic profiling involves identification and quantification of protein components in complex biological systems. Most of the mass profiling studies performed with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) have been restricted to peptides and small proteins (<20 kDa) because the sensitivity of the standard ion detectors decreases with increasing ion mass. Here we perform a protein profiling study of the snake venom Sistrurus miliarius barbouri, comparing 2D gel electrophoresis and reversed-phase high-performance liquid chromatography (HPLC) with a high mass cryodetector MALDI-TOF instrument (Macromizer), whose detector displays an uniform sensitivity with mass. Our results show that such MS approach can render superior analysis of protein complexity compared with that obtained with the electrophoretic and chromatographic approaches. The summation of ion impacts allows relative quantification of different proteins, and the number of ion counts correlates with the peak areas in the reversed-phase HPLC. Furthermore, the sensitivity reached with the high mass cryodetection MS technology clearly exceeds the detection limit of standard high-sensitivity staining methods.
Yanes
Oscar
O
Institut de Biotecnologia i Biomedicina and Departament de Bioquimica i Biol. Mol., Universitat Autònoma de Barcelona, Bellaterra (Barcelona), Spain.
Avilés
Francesc X
FX
Wenzel
Ryan
R
Nazabal
Alexis
A
Zenobi
Renato
R
Calvete
Juan J
JJ
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003435:Crotalid Venoms / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D008969:Molecular Sequence Data; D040901:Proteomics / Q000379:methods*; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D017819:Viperidae
D003435:Crotalid Venoms