[PPARalpha agonist--fenofibrate inhibits LPS-induced tissue factor expression in THP-1 cells].
This study was aimed to investigate the influence of PPARalpha agonist on the expression of TF (tissue factor) in THP-1 cells. THP-1 cells were pretreated with different concentrations of PPARalpha agonist (fenofibrate) for definite time. Lipopolysaccharide (LPS)-induced TF mRNA and protein levels were detected by RT-PCR and Western blot respectively. The results showed that fenofibrate decreased tissue factor protein and mRNA expression in supernatants of LPS-stimulated human monocytes in a concentration-dependent manner (P < 0.05 - 0.01, n = 5). It is concluded that fenofibrate inhibit TF expression induced by LPS in THP-1 cells, which may be involved in the anti-atherosclerotic effects of PPARalpha agonist.
Dong
Chun-Xia
CX
Institut of Hematology, Union Hospital, Tongji Medical College, Huazhong Univesity of Science and Technology, Wuhan 430022, China.
Hu
Yu
Y
Wang
Hua-Fang
HF
Sun
Chun-Yan
CY
Wang
Ya-Dan
YD
He
Wen-Juan
WJ
Zhang
Xiao-Ping
XP
D003864:Depression, Chemical; D011345:Fenofibrate / Q000494:pharmacology*; D006801:Humans; D007948:Leukemia, Monocytic, Acute / Q000378:metabolism* / Q000473:pathology; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000494:pharmacology; D047493:PPAR alpha / Q000819:agonists*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013925:Thromboplastin / Q000096:biosynthesis* / Q000235:genetics; D014407:Tumor Cells, Cultured
D008070:Lipopolysaccharides; D047493:PPAR alpha; D012333:RNA, Messenger; D013925:Thromboplastin; D011345:Fenofibrate