title: Efficient extraction of thioreodoxin from Saccharomyces cerevisiae by ethanol.
abstract: Thioredoxin, an antioxidant protein, is a promising molecule for development of functional foods because it protects the gastric mucosa and reduces the allergenicity of allergens. To establish a method for obtaining an ample amount of yeast thioredoxin, we found here that thioredoxin is released from Saccharomyces cerevisiae by treatment with 20% ethanol. We also found that Japanese sake contains a considerable amount of thioredoxin.
authors.affiliation: Laboratory of Molecular Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan. y_inoue@kais.kyoto-u.ac.jp
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Inoue
authors.forename: Wataru
authors.initials: W
authors.lastname: Nomura
authors.forename: Yoko
authors.initials: Y
authors.lastname: Takeuchi
authors.forename: Takumi
authors.initials: T
authors.lastname: Ohdate
authors.forename: Shogo
authors.initials: S
authors.lastname: Tamasu
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kitaoka
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Kiyokawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Masutani
authors.forename: Kazuo
authors.initials: K
authors.lastname: Murata
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Wakai
authors.forename: Shingo
authors.initials: S
authors.lastname: Izawa
authors.forename: Junji
authors.initials: J
authors.lastname: Yodoi
mesh_terms: D000431:Ethanol / Q000494:pharmacology*; D005285:Fermentation; D005656:Fungal Proteins / Q000032:analysis / Q000302:isolation & purification; D007218:Industrial Microbiology / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000201:enzymology* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins / Q000032:analysis / Q000302:isolation & purification; D013879:Thioredoxins / Q000032:analysis / Q000302:isolation & purification*; D014920:Wine / Q000032:analysis
chemical_list: D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D000431:Ethanol; D013879:Thioredoxins