[Effect of daqingye on EP3 mRNA expression of hypothalamus in IL-1beta-induced rabbits].
OBJECTIVE
To study the antipyretic effect and mechanism of Daqingye injection (DQYI).


METHODS
The IL-1beta-induced febrile New Zealand rabbits were chosen as experimental models and the antipyretic effect of DQYI was observed. The expression of EP3 mRNA was investigated by using in situ hybridization (ISH) in POAH.


RESULTS
First, the colonic temperature went up gradually after intravenous(i.v) IL-1beta. The peak value of temperature(deltaT) and thermal response index (TRI(1)) in IL-1beta-treated group were higher than those in control group (P<0.01). The DeltaT and TRI(1) of in DQYI and IL-1beta- treated group were lower than those in IL-1beta-treated group (P<0.05). The temperature of DQYI-treated group showed no distinguished difference compared with that in control group (P>0.05). Second, the expression of EP3 mRNA in the POAH of IL- 1beta-treated group increased markedly compared with that in control group (P<0.01). The expression of EP3 mRNA treated by IL-1beta+DQYI in the POAH decreased strikingly compared with that in IL-1beta group(P<0.05).


CONCLUSIONS
DQYI has distinct antipyretic effect on IL-1beta-induced fever. The mechanism might be the inhibition of EP3 mRNA expression in POAH from rabbits.
Dong
Jun
J
Department of Pathophysiology, Medical College of Jinan University, Guangzhou 510632, China. dongjunbox@163.com
Qiu
Sheng
S
Xie
Xin-hua
XH
Pan
Rui
R
Tang
Hong-mei
HM
Lu
Da-xiang
DX
D018712:Analgesics, Non-Narcotic / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D001831:Body Temperature / Q000187:drug effects; D003106:Colon / Q000187:drug effects / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism*; D017403:In Situ Hybridization; D053583:Interleukin-1beta / Q000494:pharmacology*; D008297:Male; D012333:RNA, Messenger; D011817:Rabbits; D018078:Receptors, Prostaglandin E / Q000235:genetics*
D018712:Analgesics, Non-Narcotic; D004365:Drugs, Chinese Herbal; D053583:Interleukin-1beta; D012333:RNA, Messenger; D018078:Receptors, Prostaglandin E