Multiresidue determination of zeranol and related compounds in bovine muscle by gas chromatography/mass spectrometry with immunoaffinity cleanup.
A gas chromatography/mass spectrometry (GC/MS) method with immunoaffinity cleanup was developed for the determination of zeranol and related compounds, taleranol, zearalanone, and alpha-zearalenol in bovine muscle. Muscle samples were extracted with methanol and cleaned up with immunoaffinity chromatography (IAC) columns containing monoclonal antibodies raised against zeranol coupled to CNBr-activated Sepharose 4B. After derivatization, the compounds were analyzed by GC/MS. The dynamic column capacities for zeranol, taleranol, zearalanone, and alpha-zearalenol were 2639.7, 2840.3, 2731.5, and 2736.3 ng/mL Sepharose gel, respectively. The limits of detection and quantification were 0.5 and 1.0 ng/g, respectively, for all 4 compounds. Mean recoveries were 79.6-110.7% with coefficients of variation of 3.2-11.4% at spiked levels of 1.0-5.0 ng/g. This IAC-GC/MS method may be used for the determination of zeranol, taleranol, zearalanone, and alpha-zearalenol residues in bovine muscle, and possibly other tissues.
Zhang
Wei
W
China Agricultural University, College of Veterinary Medicine, Department of Pharmacology and Toxicology, Beijing 100094, People's Republic of China.
Wang
Hejia
H
Wang
Jianping
J
Li
Xiaowei
X
Jiang
Haiyang
H
Shen
Jianzhong
J
D000818:Animals; D002138:Calibration; D002417:Cattle; D002846:Chromatography, Affinity; D004350:Drug Residues / Q000032:analysis*; D004968:Estrogens, Non-Steroidal / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D007120:Immunochemistry; D007202:Indicators and Reagents; D018482:Muscle, Skeletal / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D015029:Zeranol / Q000032:analysis*
D004968:Estrogens, Non-Steroidal; D007202:Indicators and Reagents; D015029:Zeranol