The effect of copper deficiency on the formation of hemosiderin in sprague-dawley rats.
We demonstrated previously that loading iron into ferritin via its own ferroxidase activity resulted in damage to the ferritin while ferritin loaded by ceruloplasmin, a copper-containing ferroxidase, was not damaged and had similar characteristics to native ferritin (Welch et al. (2001) Free Radic Biol Med 31:999-1006). Interestingly, it has been suggested that the formation of hemosiderin, a proposed degradation product of ferritin, is increased in animals deficient in copper. In this study, groups of rats were fed normal diets, copper deficient diets, iron supplemented diets, or copper deficient-iron supplemented diets for 60 days. Rats fed copper-deficient diets had no detectable active serum ceruloplasmin, which indicates that they were functionally copper deficient. There was a significant increase in the amount of iron in isolated hemosiderin fractions from the livers of copper-deficient rats, even more than that found in rats fed only an iron-supplemented diet. Histological analysis showed that copper-deficient rats had iron deposits (which are indicative of hemosiderin) in their hepatocytes and Kupffer cells, whereas rats fed diets sufficient in copper only had iron deposits in their Kupffer cells. Histologic evidence of iron deposition was more pronounced in rats fed diets that were deficient in copper. Additionally, sucrose density-gradient sedimentation profiles of ferritin loaded with iron in vitro via its own ferroxidase activity was found to have similarities to that of the sedimentation profile of the hemosiderin fraction from rat livers. The implications of these data for the possible mechanism of hemosiderin formation are discussed.
Welch
Kevin D
KD
Biotechnology Center, Utah State University, Logan, UT 84322-4705, USA.
Hall
Jeffery O
JO
Davis
T Zane
TZ
Aust
Steven D
SD
D000821:Animal Feed; D000818:Animals; D002499:Centrifugation, Density Gradient; D002570:Ceruloplasmin / Q000378:metabolism; D003300:Copper / Q000737:chemistry* / Q000172:deficiency; D006485:Hemosiderin / Q000737:chemistry / Q000378:metabolism*; D022781:Hepatocytes / Q000378:metabolism; D006801:Humans; D007501:Iron / Q000737:chemistry; D007728:Kupffer Cells / Q000378:metabolism; D008297:Male; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013395:Sucrose / Q000494:pharmacology
D013395:Sucrose; D003300:Copper; D006485:Hemosiderin; D007501:Iron; D002570:Ceruloplasmin; C022345:4-phenylenediamine oxidase; D000587:Oxidoreductases Acting on CH-NH Group Donors