title: Screening of periodontitis with salivary enzyme tests.
abstract: The purpose of this study was to determine the usefulness of salivary biochemical markers for the screening of periodontal disease and examine the agreement between the results of saliva enzyme tests and those of probing depth. The present study included a total of 187 subjects who underwent annual medical check-ups at the Comprehensive Health Care Center, Honjo, Saitama Prefecture, Japan. Periodontal pocket probing was performed with a WHO probe, and various enzymes and biochemical parameters in saliva were measured. For lactate dehydrogenase (LDH), the proportions of the five isoenzymes were calculated. To decide the cut-off point for each enzymatic activity, receiver operating characteristic curves (ROC curves) were constructed and the points of minimum difference between sensitivity and specificity were decided. Among the biochemical markers tested, salivary LDH level had the highest sensitivity and specificity (sensitivity 0.66, specificity 0.67), while salivary levels of aspartate aminotransferase (AST) and blood urea nitrogen (BUN) also had sensitivity and specificity above 0.60. Among the LDH isoenzymes, LDH4 and LDH5 dominated in whole saliva samples. Salivary LDH may be a feasible and useful parameter for the screening of periodontal disease, while salivary AST and BUN also appear to be potentially useful for this purpose.
authors.affiliation: Department of Preventive Dentistry and Public Health, Tsurumi University School of Dental Medicine, Yokohama, Kanagawa, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Nomura
authors.forename: Yoh
authors.initials: Y
authors.lastname: Tamaki
authors.forename: Tomoko
authors.initials: T
authors.lastname: Tanaka
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Arakawa
authors.forename: Akihisa
authors.initials: A
authors.lastname: Tsurumoto
authors.forename: Kazuko
authors.initials: K
authors.lastname: Kirimura
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Sato
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Hanada
authors.forename: Kyuichi
authors.initials: K
authors.lastname: Kamoi
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D001219:Aspartate Aminotransferases / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D001806:Blood Urea Nitrogen; D004796:Clinical Enzyme Tests; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000032:analysis; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D008297:Male; D008403:Mass Screening / Q000379:methods; D008875:Middle Aged; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000473:pathology; D010517:Periodontics / Q000295:instrumentation; D010518:Periodontitis / Q000175:diagnosis*; D012372:ROC Curve; D012463:Saliva / Q000201:enzymology*; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases