Prevention of epimerization and quantitative determination of amygdalin in Armeniacae Semen with Schizandrae Fructus solution.
Armeniacae Semen not only contains amygdalin, but emulsin also, which is an enzyme that hydrolyzes amygdalin. The extraction yield of amygdalin from Armeniacae Semen was low, due to the presence of emulsin, when extracted with water. When Schizandrae Fructus solution was used as the extractant; however, amygdalin was almost completely extracted, regardless of the cutting size, due to the absence of the influence of emulsin. In addition, when the crude powder or small piece forms were used with Schizandrae Fructus solution, on epimerization of the D-amygdalin into neoamygdalin occurred. D-amygdalin and its conversion product, neoamygdalin, were quantitatively analyzed by reverse-phase, high-performance liquid chromatography (HPLC), with an optimized eluent of 10 mM sodium phosphate buffer (pH 2.3), containing 11.5% acetonitrile. The concentration and detector response were linearly correlated over the range 0.05 to 2 mM. The detection limits for both D-amygdalin and neoamygdalin were approximately 5 microM for the amount injected.
Joo
Woo-Sang
WS
Department of Oriental Pharmaceutical Sciences, Kyung Hee University, Seoul 130-701, Korea.
Jeong
Ji-Seon
JS
Kim
Hyogeun
H
Lee
Yong-Moon
YM
Lee
Je-Hyun
JH
Hong
Seon-Pyo
SP
D000678:Amygdalin / Q000737:chemistry* / Q000302:isolation & purification; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry; D027861:Prunus / Q000737:chemistry*; D032141:Schisandra / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D012996:Solutions; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D014867:Water
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D012996:Solutions; D012997:Solvents; D014867:Water; D000678:Amygdalin