title: MMP-7 (matrilysin) expression in human brain tumors.
abstract: Matrix metalloproteinases (MMP) which degrades protein components of the extra-cellular matrix and basement membrane seems to be largely involved in cancer invasiveness. MMP proteolitic activity essentially comes from stromal cells but matrilysin (MMP-7) is produced by the tumor itself. Thus, MMP-7 is investigated to address the particular invasive behavior of human glioma. Both MMP-7 mRNA and protein were clearly identified in human glioma. MMP-7 mRNA expression was highly variable within our glioma population. When analyzing MMP-7 mRNA expression in different primary brain tumors, we found highly variable levels of expression not related to their invasive behavior. In successive biopsies obtained in the same patients with glioblastoma, MMP-7 mRNA was quantified and appeared variable, but intra-individual variations were lower than inter-individual differences. With a xenograft model of U87 human tumors in RAG2/gamma(c) immune-deficient mice, the strict tumor origin of MMP-7 was shown. Additionally, MMP-7 expression by U87 cells which is low in culture was stimulated by these cells while forming tumors and the level of expression was higher when the tumor cells were implanted within the brain. These data provide some consistent information about cross-talk occurring between the tumor and the surrounding stroma to regulate MMP-7 expression.
authors.affiliation: Molecular and Functional Imaging, ERT-CNRS 5543, Université Victor Segalen, Bordeaux, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Rome
authors.forename: Josette
authors.initials: J
authors.lastname: Arsaut
authors.forename: Caroline
authors.initials: C
authors.lastname: Taris
authors.forename: Franck
authors.initials: F
authors.lastname: Couillaud
authors.forename: Hugues
authors.initials: H
authors.lastname: Loiseau
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology; D045744:Cell Line, Tumor; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000201:enzymology; D005910:Glioma / Q000201:enzymology*; D006801:Humans; D020783:Matrix Metalloproteinase 7 / Q000096:biosynthesis*; D051379:Mice; D009368:Neoplasm Transplantation; D012333:RNA, Messenger / Q000378:metabolism; D012334:RNA, Neoplasm / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D012334:RNA, Neoplasm; D020783:Matrix Metalloproteinase 7