Long polymeric tips of atomic force microscopy for large biological samples.
We show a new atomic force microscopy technique for obtaining high-resolution topographic images of large bio-samples. To obtain high-resolution topographic images for the samples, we fabricated a long polymeric tip with a small protrusion using two-photon adsorbed photo-polymerization techniques. The obtained tip length was over 50 microm, and the tip was used directly to visualize COS-1 and 293 cells. Compared with commercial tips, the long tip made it easier to obtain topographic images of the large cells. In the magnified topographic images, the sub-100-nm resolution was confirmed with the long tips. This long probe tip is expected to broaden large sample-related studies and applications in the future.
Muramatsu
Hiroshi
H
School of Bionics, Tokyo University of Technology, Katakura, Hachioji, Tokyo 192-0982, Japan.
Yamamoto
Yuji
Y
Sato
Atsushi
A
Enomoto
Shizuo
S
Kim
Woo-Sik
WS
Chang
Sang-Mok
SM
Kim
Jong Min
JM
D000818:Animals; D019556:COS Cells; D002460:Cell Line; D002522:Chlorocebus aethiops; D004867:Equipment Design; D006801:Humans; D007668:Kidney / Q000166:cytology*; D018625:Microscopy, Atomic Force / Q000295:instrumentation* / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D012117:Resins, Synthetic / Q000737:chemistry*; D013499:Surface Properties
D011108:Polymers; D012117:Resins, Synthetic