title: Transposon-tagged mutagenesis in the rat.
abstract: Although the laboratory rat (Rattus norvegicus) is an indispensable experimental animal for biomedical research and drug development, the lack of embryonic stem cell lines hampers gene-knockout studies. Here we report the successful generation of insertional mutant rats using the Sleeping Beauty (SB) transposon system. This would benefit a variety of biomedical research fields for which the rat model is better suited than the mouse model.
authors.affiliation: Laboratory of Mammalian Genetics, Division of Genome Dynamics, Creative Research Initiative Sousei, Hokkaido University, North 10 West 8, Kita-ku, Sapporo 060-0810, Japan. kkitada@cast.hokudai.ac.jp
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kitada
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ishishita
authors.forename: Keiko
authors.initials: K
authors.lastname: Tosaka
authors.forename: Ri-ichi
authors.initials: R
authors.lastname: Takahashi
authors.forename: Masatsugu
authors.initials: M
authors.lastname: Ueda
authors.forename: Vincent W
authors.initials: VW
authors.lastname: Keng
authors.forename: Kyoji
authors.initials: K
authors.lastname: Horie
authors.forename: Junji
authors.initials: J
authors.lastname: Takeda
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics; D000818:Animals; D030801:Animals, Genetically Modified; D004251:DNA Transposable Elements; D005260:Female; D005804:Genes, Lethal; D049452:Green Fluorescent Proteins / Q000235:genetics; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D006720:Homozygote; D008297:Male; D051379:Mice; D023421:Models, Animal; D016254:Mutagenesis, Insertional; D009419:Nerve Tissue Proteins / Q000235:genetics; D051381:Rats / Q000235:genetics*; D011971:Receptors, Immunologic / Q000235:genetics; D019895:Transposases / Q000235:genetics; D000097905:Roundabout Proteins
chemical_list: D000251:Adenosine Triphosphatases; D004251:DNA Transposable Elements; D049452:Green Fluorescent Proteins; D018840:HSP70 Heat-Shock Proteins; D009419:Nerve Tissue Proteins; D011971:Receptors, Immunologic; D019895:Transposases; C516057:Robo2 protein, rat; D000097905:Roundabout Proteins; C517966:Hspa13 protein, rat