Efficacy of bioactive polypeptides on bleeding and intra-abdominal adhesions.
BACKGROUND
Perioperative bleeding and postoperative adhesions are two problems encountered in abdominal surgery. Commercial products are available that decrease both bleeding and development of abdominal adhesions, but no products are effective in both situations. The combination of differently charged bioactive polypeptides, administered intraperitoneally, has previously been shown effective in decreasing postoperative adhesions. The present study is a pilot examination of the effects on perioperative bleeding and postoperative adhesions, applying the polypeptide concept.


METHODS
Standardized wounds in the liver and spleen were induced in 52 NMRI mice. The amounts of bleeding and postoperative adhesions were measured after 1 and 7 days, respectively. Separate animals were examined after 8 weeks for long-term healing of the parenchymal wounds.


RESULTS
Both parenchymal bleeding and the extent of adhesions significantly decreased (p = 0.001 and p = 0.029, respectively) as compared to controls. Histology after 8 weeks showed no clear signs of impaired or altered healing.


CONCLUSIONS
Intraperitoneal administration of differently charged polypeptides significantly decreased postoperative bleeding and postoperative adhesions. Bioactive polypeptides appear promising in the promotion of peritoneal healing and merits further studies.
Tingstedt
Bobby
B
Department of Surgery and Physical Chemistry 1, Centre of Chemistry, Lund University, Lund, Sweden. bobby.tingstedt@med.lu.se
Nehéz
László
L
Lindman
Bjorn
B
Andersson
Roland
R
D000005:Abdomen / Q000601:surgery; D000818:Animals; D018698:Glutamic Acid / Q000494:pharmacology; D007274:Injections, Intraperitoneal; D007431:Intraoperative Complications / Q000188:drug therapy / Q000473:pathology / Q000517:prevention & control; D008099:Liver / Q000601:surgery; D008239:Lysine / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D010455:Peptides / Q000494:pharmacology*; D019106:Postoperative Hemorrhage / Q000188:drug therapy* / Q000473:pathology / Q000517:prevention & control; D013154:Spleen / Q000601:surgery; D000267:Tissue Adhesions / Q000188:drug therapy* / Q000473:pathology / Q000517:prevention & control; D014945:Wound Healing / Q000187:drug effects*
D010455:Peptides; D018698:Glutamic Acid; D008239:Lysine