Effect of a bone marrow microenvironment on the ex-vivo expansion of umbilical cord blood progenitor cells.
Progenitor cells (CD34(+)) can be isolated from umbilical cord blood and used to correct or reconstitute various cell lines within the haematopoietic and endothelial cell lineage. The main disadvantage of this procedure relates to the low volume of blood that can be collected after the umbilical cord has been clamped, which limits the number of progenitor cells available for treatment. This limitation, however, can be overcome by expanding CD34(+) cells ex vivo. Our aim was to perform a controlled study to determine if the ex-vivo proliferation of umbilical cord CD34(+) cells is enhanced when they are placed in a system that mimics the bone marrow microenvironment. For this purpose, CD34(+) cells were isolated from umbilical cord blood using a magnetic cell sorting kit and seeded in platforms containing different cocktails of cytokines with and without a three-dimensional (3D) biomatrix. Results from this study suggest that the number of viable cells can double after 1 week in any of the culture platforms and that the 3D biomatrix does not enhance cell proliferation.
Galan
I
I
The Women's Health Research Institute, Texas Tech University Health Science Center at Amarillo, Amarillo, TX, USA.
DeLeon
J A
JA
Diaz
L
L
Hong
J-S
JS
Khalek
N
N
Munoz-Fernandez
M A
MA
Santolaya-Forgas
J
J
D018952:Antigens, CD34; D001853:Bone Marrow; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans
D018952:Antigens, CD34