title: Biodegradation of crystal violet by Pseudomonas putida.
abstract: Crystal violet (CV), which has been extensively used as a biological stain and a commercial textile dye, is a recalcitrant molecule. A strain of Pseudomonas putida was isolated that effectively degraded CV: up to 80% of 60 microM CV as the sole carbon source, was degraded in liquid media within 1 week. Nine degradation products were isolated and identified. We propose that CV degradation occurs via a stepwise demethylation process to yield mono-, di-, tri-, tetra-, penta- and hexa-demethylated CV species.
authors.affiliation: Department of General Education, National Taichung Nursing College, Taichung, Taiwan.
authors.forename: Chiing-Chang
authors.initials: CC
authors.lastname: Chen
authors.forename: Hung-Ju
authors.initials: HJ
authors.lastname: Liao
authors.forename: Chiu-Yu
authors.initials: CY
authors.lastname: Cheng
authors.forename: Chia-Yuen
authors.initials: CY
authors.lastname: Yen
authors.forename: Ying-Chien
authors.initials: YC
authors.lastname: Chung
mesh_terms: D001673:Biodegradation, Environmental; D005840:Gentian Violet / Q000378:metabolism*; D016958:Pseudomonas putida / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D018508:Water Purification / Q000379:methods*
chemical_list: D014874:Water Pollutants, Chemical; D005840:Gentian Violet