title: [Determination of glycarbylamide based on formation of nickel chelate].
abstract: A new and simple analytical method for glycarbylamide (GB) based on the formation of nickel chelate was developed. The proposed method is as follows: sample solution is mixed with 0.08 mmol/L nickel nitrate in 0.2 mol/L carbonate buffer (pH 9.0), and the absorbance is measured at 290 nm. Under the optimal conditions, GB could be determined in the concentration range from 0.13 microg/mL to 2.6 microg/mL (r = 0.9999). Using this method for HPLC post column reaction, GB levels in chicken liver extracts could be determined. The recovery of GB was 79.4% (RSD=2.6%, n=3) and the quantitation limit was 30 ng/g. The apparent molar extinction coefficient (epsilon) of the GB-nickel complex was 8.5 x 10(3). The molar ratio of the complex is GB: nickel ion = 2:1.
authors.affiliation: Showa University, School of Pharmaceutical Sciences, 1-5-8, Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Tajima
authors.forename: Yasuharu
authors.initials: Y
authors.lastname: Mizuno
authors.forename: Kouko
authors.initials: K
authors.lastname: Hamamoto
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Arakawa
authors.forename: Masako
authors.initials: M
authors.lastname: Maeda
mesh_terms: D000818:Animals; D002614:Chelating Agents; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003049:Coccidiostats / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D007093:Imidazoles / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D009532:Nickel
chemical_list: D002614:Chelating Agents; D003049:Coccidiostats; D007093:Imidazoles; C035197:nickel nitrate; D009532:Nickel; C005006:glycarbylamide