Biocompatibility of different barrier membranes in cultures of human CRL 11372 osteoblast-like cells: an immunohistochemical study.
OBJECTIVE
The aim of this study was to evaluate the biocompatibility of two different barrier materials in cultures of human osteoblast-like cells (CRL 11372) in vitro.


METHODS
Polylactic acid (Epi-Guide; EG) and collagen membrane (Bio-Collagen, BC) were examined. To analyze the effect of materials on cell proliferation, cell numbers and cell viability, cells were cultured on the barrier membranes for 24 and 72 h. Cells plated on culture dishes (CD) served as positive controls. Cell proliferation rate was assessed by the bromodeoxyuridine (BrdU) immunohistochemical technique. The cell numbers of each well were counted. Cell viability was estimated by counting the number of cells, which excluded trypan blue solution. Scanning electron microscopy (SEM) was used to observe the interactions between osteoblastic cells and barrier membranes.


RESULTS
The highest number of BrdU-labeled cells were seen on CD after both of the time periods. In comparison with the other two groups, BC showed significantly fewer cells after both time periods. Regarding cell numbers, after 24 and 72 h of incubations CD showed the highest number of cells. The number of viable cells was similar for all the groups. After 72 h for the EG group, SEM view showed flat cells. After 72-h time periods, the BC group revealed a weak adhesion of cells to the barriers.


CONCLUSIONS
The results demonstrate that cells were able to proliferate on these materials and EG promoted the proliferation of human osteoblast like cells. We prefer to use the EG membrane.
Bilir
Ayhan
A
Department of Histology and Embriology, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey.
Aybar
Buket
B
Tanrikulu
Sinasi H
SH
Issever
Halim
H
Tuna
Sevilcan
S
D000963:Antimetabolites; D001672:Biocompatible Materials / Q000494:pharmacology*; D001973:Bromodeoxyuridine; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology; D004396:Coloring Agents; D006801:Humans; D007150:Immunohistochemistry; D019344:Lactic Acid / Q000494:pharmacology; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000187:drug effects*; D011091:Polyesters; D011108:Polymers / Q000494:pharmacology; D013499:Surface Properties; D013997:Time Factors; D014343:Trypan Blue
D000963:Antimetabolites; D001672:Biocompatible Materials; D004396:Coloring Agents; D008567:Membranes, Artificial; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D003094:Collagen; D001973:Bromodeoxyuridine; D014343:Trypan Blue