title: In vivo treatment by diallyl disulfide increases histone acetylation in rat colonocytes.
abstract: Diallyl disulfide (DADS) is an organosulfur compound from garlic which exhibits various anticarcinogenic properties including inhibition of tumor cell proliferation. DADS antiproliferative effects were previously associated with an increase in histone acetylation in two human tumor colon cell lines, suggesting that DADS-induced histone hyperacetylation could be one of the mechanisms involved in its protective properties on colon carcinogenesis. The effects of DADS on histone H4 and H3 acetylation levels were investigated in vivo in colonocytes isolated from non-tumoral rat. Administrated by intracaecal perfusion or gavage, DADS increases histone H4 and H3 acetylation in colonocytes. Moreover, data generated using cDNA expression arrays suggest that DADS could modulate the expression of a subset of genes. These results suggest the involvement of histone acetylation in modulation of gene expression by DADS in normal rat colonocytes, which might play a role in its biological effects as well as in its anticarcinogenic properties in vivo.
authors.affiliation: Laboratoire de Nutrition et Sécurité Alimentaire, INRA, Domaine de Vilvert, 78352 Jouy-en-Josas cedex, France. Nathalie.Pecollo@jouy.inra.fr
authors.forename: Nathalie
authors.initials: N
authors.lastname: Druesne-Pecollo
authors.forename: Catherine
authors.initials: C
authors.lastname: Chaumontet
authors.forename: Anthony
authors.initials: A
authors.lastname: Pagniez
authors.forename: Pierre
authors.initials: P
authors.lastname: Vaugelade
authors.forename: Aurélia
authors.initials: A
authors.lastname: Bruneau
authors.forename: Muriel
authors.initials: M
authors.lastname: Thomas
authors.forename: Claire
authors.initials: C
authors.lastname: Cherbuy
authors.forename: Pierre-Henri
authors.initials: PH
authors.lastname: Duée
authors.forename: Paule
authors.initials: P
authors.lastname: Martel
mesh_terms: D000107:Acetylation / Q000187:drug effects; D000498:Allyl Compounds / Q000008:administration & dosage*; D000818:Animals; D002478:Cells, Cultured; D003106:Colon / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004220:Disulfides / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D004750:Enteral Nutrition; D015870:Gene Expression / Q000187:drug effects; D006657:Histones / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D020543:Proteome / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000498:Allyl Compounds; D004220:Disulfides; D006657:Histones; D020543:Proteome; C028009:diallyl disulfide