A branched-chain organic acid linked to multiple sclerosis: first identification by NMR spectroscopy of CSF.
(1)H NMR spectroscopy of cerebrospinal fluid (CSF) is currently being used to study metabolic profiles characteristic of distinct multiple sclerosis (MS) manifestations. For select MS patient groups, we have previously detected significantly increased concentrations of several identified metabolites and one unidentified compound. We now present, for the first time, the identification of the latter molecule, beta-hydroxyisobutyrate (BHIB). A combination of dedicated 1D and 2D (1)H NMR experiments was employed for signal assignment. To our knowledge, BHIB has not previously been identified in (1)H NMR spectra of biofluids or biological tissues. Our assignment suggests new biochemical pathways involved in specific MS pathologies.
Lutz
Norbert W
NW
Centre de Résonance Magnétique Biologique et Médicale (CRMBM), UMR CNRS 6612, Faculté de Médecine, Université de la Méditerranée, Marseille, France. Norbert.Lutz@medecine.univ-mrs.fr
Viola
Angèle
A
Malikova
Irina
I
Confort-Gouny
Sylviane
S
Ranjeva
Jean-Philippe
JP
Pelletier
Jean
J
Cozzone
Patrick J
PJ
D000143:Acids / Q000134:cerebrospinal fluid / Q000737:chemistry; D015415:Biomarkers / Q000134:cerebrospinal fluid; D006801:Humans; D006885:Hydroxybutyrates / Q000134:cerebrospinal fluid* / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D009103:Multiple Sclerosis / Q000378:metabolism*; D009930:Organic Chemicals / Q000134:cerebrospinal fluid / Q000737:chemistry; D011522:Protons
D000143:Acids; D015415:Biomarkers; D006885:Hydroxybutyrates; D009930:Organic Chemicals; D011522:Protons