[Effects of bone marrow mesenchymal stem cells enriched by small intestinal submucosal films on cardiac function and compensatory circulation after myocardial infarction in goats].
OBJECTIVE
To investigate effects of the autologous bone mesenchymal stem cells (MSCs) enriched by the small intestinal submucosa (SIS) film implantation on the myocardial structure, cardiac function, and compensatory circulation after myocardial infarction in the goats.


METHODS
Sixteen black goats were selected and divided randomly into the control group (n= 8)and the experimental group (n= 8). The chronic myocardial infarction models were made by the ligation of the far end of the left anterior descending coronary artery. At the same time, MSCs were aspired from the thigh bone of the goats in the experimental group. MSCs were isolated by the centrifugation through a percoll step gradient and purified by the plating culture and depletion of the non-adherent cells. Primary MSCs were cultured in the DMEM medium supplemented with the fetal bovine serum in vitro. After that, the cultures were labeled by 5- BrdU. The active cells were transplanted into the SIS film. Six weeks after the ligation, the MSCs-SIS film was implanted by its being sutured onto the infarction area; whereas, the control group underwent a sham-operation. In both groups, echocardiographic measurements were performed before infarction, 6 weeks after infarction and 6 weeks after the MSC-collagen implantation, respectively, to assess the myocardial structure and cardiac function. The left coronary artery angiography was performed with the digital subtraction angiography.


RESULTS
In an assessment of the left ventricular function, at 6 weeks after operation, the stroke volume and the ejection fraction of the control group and the experimental group were 42.81+/-4.91, 37. 06+/-4.75 ml and 59.20%+/-5.41%, 44.56% +/-4.23%, respectively (P<0.05). The end-diastolic volume and the end-systolic volume of the control group and the experimental group were 72.55+/-8.13, 83.31+/-8.61 ml and 29.75+/-5. 98, 46.25+/-6.68 ml, respectively (P<0. 05). The maximal velocity of peak E of control group and experimental group were 54. 85+/-6.35 cm/s and 43.14+/-4.81cm/s (P<0. 01); and the maximal velocity of peak A of control group and experimental group were 52.33+/-6.65 cm/s and 56.91+/-6.34 cm/s (P>0. 05). Echocardiography showing a distinctly dilatation of left ventricle with the ventricular dyskinesia in control group, but without the ventricular dyskinesia in experimental group. The selective-coronary angiography revealed that the obvious compensatory circulation established between the anterior descending branch and the left circumflex branch in the experimental group.


CONCLUSIONS
Implantation of the autologous MSCs enriched by the SIS film can prevent dilatation of the left ventricular chamber and can improve the contractile ability of the myocardium, cardiac function, and collateral perfusion.
Liao
Bin
B
Department of Thoracic and Cardiovascular Surgery, Affiliated Hospital of Luzhou Medical College, Luzhou Sichuan, 646000, PR China. liaobindr@tom.com
Deng
Li
L
Wang
Feng
F
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005260:Female; D006041:Goats; D007421:Intestine, Small; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009203:Myocardial Infarction / Q000601:surgery*; D023822:Tissue Engineering; D054457:Tissue Scaffolds