Theoretical modelling of radiolytic damage of free DNA bases and within DNA macromolecule.
The base damages in living cell are the most frequent product of deleterious effect of ionizing radiation. Experimental yields of modified bases determined in free bases and in the double stranded DNA show significant differences in the yields of stable products of radiolytic attack. An attempt to explain these differences is made with the help of theoretical calculations. The relative probabilities of reactions of the most important radiolytic product, OH(*) radical, with individual nucleobases, nucleosides and short DNA oligomers are calculated and compared to available experimental yields of base damages.
Stepán
Václav
V
Faculty of Nuclear Sciences and Physical Engineering, Department of Dosimetry and Application of Ionizing Radiation, Czech Technical University, Brehová, Prague, Czech Republic.
Davídková
Marie
M
D003198:Computer Simulation; D004247:DNA / Q000737:chemistry* / Q000528:radiation effects*; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D046911:Macromolecular Substances / Q000737:chemistry / Q000528:radiation effects; D008956:Models, Chemical; D008958:Models, Molecular; D009711:Nucleotides / Q000737:chemistry* / Q000528:radiation effects*; D011829:Radiation Dosage
D046911:Macromolecular Substances; D009711:Nucleotides; D004247:DNA