Direct modification of mRNA by ferrocenyl carbodiimide and its application to electrochemical detection of mRNA.
Ferrocenyl carbodiimide (1) could be used for the direct labeling of synthetic RNA and expressed mRNA in vitro with the electrochemically active ferrocene moieties. These RNAs modified by 1 could be detected electrochemically coupled with a DNA probe-immobilized electrode. After hybridization of 1.1 Kb mRNA modified by 1 with the DNA probe-immobilized electrode, the peak charge observed by an Osteryoung square wave voltammetry (SWV) measurement correlated well with the concentration of mRNA, having a detection limit at the sub nanogram level.
Mukumoto
Kosuke
K
Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka, Japan.
Nojima
Takahiko
T
Sato
Shinobu
S
Waki
Michinori
M
Takenaka
Shigeori
S
D002234:Carbodiimides / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D015342:DNA Probes; D018076:DNA, Complementary / Q000737:chemistry; D004563:Electrochemistry; D005296:Ferrous Compounds / Q000737:chemistry*; D007202:Indicators and Reagents; D012333:RNA, Messenger / Q000032:analysis* / Q000138:chemical synthesis / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D002234:Carbodiimides; D015342:DNA Probes; D018076:DNA, Complementary; D005296:Ferrous Compounds; D007202:Indicators and Reagents; D012333:RNA, Messenger; C517870:ferrocenyl carbodiimide