Spectroscopic, viscositic and electrochemical studies of DNA interaction with a novel mixed-ligand complex of nickel (II) that incorporates 1-methylimidazole and thiocyanate groups.
A novel mixed-ligand nickel(II) complex that contains 1-methylimidazole and thiocyanate, Ni(NCS)(2)(Mim)(4) (Mim=1-methylimidazole), was synthesized and its structure was determined by X-ray crystallography, IR spectrum and elemental analysis, etc. Its DNA-binding properties were studied by electronic absorption spectral, viscositive and electrochemical measurements. The absorption spectral and viscositive results suggest that the nickel(II) complex binds to DNA via partial intercalation. The addition of DNA results in the decrease of the peak current of the nickel(II) complex proved their interaction. The slight differences of peak profiles and electrochemical parameters between free and DNA-bound Ni(NCS)(2)(Mim)(4) showed the formation of an electrochemical inactive complex between Ni(NCS)(2)(Mim)(4) and DNA. The binding site and binding constant of the complex to DNA were determined by electrochemical titration method.
Wang
Qing-Xiang
QX
College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China.
Jiao
Kui
K
Liu
Fa-Qian
FQ
Yuan
Xian-Long
XL
Sun
Wei
W
D000818:Animals; D001665:Binding Sites; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry; D007093:Imidazoles / Q000737:chemistry; D008024:Ligands; D009532:Nickel / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013861:Thiocyanates / Q000737:chemistry; D014783:Viscosity
D007093:Imidazoles; D008024:Ligands; D009942:Organometallic Compounds; D013861:Thiocyanates; D009532:Nickel; D004247:DNA