title: Transcription activity of ectogenic human carcinoembryonic antigen promoter in lung adenocarcinoma cells A549.
abstract: The transcription activity of ectogenic human carcinoembryonic antigen (CEA) promoter in lung adenocarcinoma cells A549 was investigated for the further gene-targeting therapy. The reporter gene green fluorescent protein (GFP) driven by CEA promoter and human cytomegalovirus (CMV) promoter were relatively constructed and named plasmid pCEA-EGFP and pCMV-GFP respectively. The intensity of fluorescence was detected by fluorescence microscope and flow cytometry analysis after the pCEA-GFP and pSNAV-GFP plasmids were transfected into A549 cells through liposome respectively. The results showed (4.08+/-0.63) % of the A549 cells transfected with pCEA-AFP plasmid expressed, significantly lower than that of the A549 cells transfected with pCMV-GFP [(43.27+/-3.54) %]. It was suggested that ectogenic human CEA promoter in lung adenocarcinoma cells A549 was weakly expressed. The distinct specificity of CEA promoter in CEA high expression cells was regarded as a tool in selective gene therapy, but the transcription activity of ectogenic human CEA promoter was needed to increase in the future.
authors.affiliation: Department of Respiratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Weining
authors.initials: W
authors.lastname: Xiong
authors.forename: Huijuan
authors.initials: H
authors.lastname: Fang
authors.forename: Yongjian
authors.initials: Y
authors.lastname: Xu
authors.forename: Shendao
authors.initials: S
authors.lastname: Xiong
authors.forename: Yong
authors.initials: Y
authors.lastname: Cao
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Song
authors.forename: Daxiong
authors.initials: D
authors.lastname: Zeng
authors.forename: Huilan
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology; D002272:Carcinoembryonic Antigen / Q000235:genetics*; D045744:Cell Line, Tumor; D003587:Cytomegalovirus / Q000235:genetics; D018390:Gene Targeting; D017930:Genes, Reporter / Q000235:genetics; D015316:Genetic Therapy; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D011401:Promoter Regions, Genetic / Q000235:genetics*; D015533:Transcriptional Activation / Q000235:genetics*
chemical_list: D002272:Carcinoembryonic Antigen; D049452:Green Fluorescent Proteins