title: [Production of specific CTL induced by exosomes derived from K562 cells].
abstract: The aim of this study was to investigate whether exosomes derived from K562 cells and human monocyte-derived dendritic cells (DCs) transfected with total RNA of K562 cells are capable of inducing antigen-specific cytotoxic T lymphocytes (CTL) responses in vitro. DCs were generated from peripheral blood mononuclear cells (PBMNC) of healthy volunteers in the presence of GM-CSF and IL-4, and then were transfected with K562 RNA by using DOTAP lipofection. Exosomes was extracted from the supernatant of DCs and K562 cells. The T cell were activated to be tumor specific CTL after DCs and exosomes were co-cultured with autologous T cells derived from healthy volunteers' PBMNC. The effect of CTL on K562 cells was detected by MTT assay. The results showed that treatment of T cells with exosomes derived from K562 cells or DCs transfected with total RNA of K562 cells could significantly promote their killing ability on K562 cells as compared with untreated T cells (P < 0.05). The killing ability of T cells treated with exosomes on K562 cells was stronger than on HL-60 cells (P < 0.05). It is concluded that the specific CTL immune response to leukemia cells can be induced by exosomes derived from K562 cells.
authors.affiliation: Department Hematology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Shao-Qian
authors.initials: SQ
authors.lastname: Chen
authors.forename: Ying
authors.initials: Y
authors.lastname: Du
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Qiao-Li
authors.initials: QL
authors.lastname: Gu
authors.forename: Yu-Min
authors.initials: YM
authors.lastname: Huang
authors.forename: Zi-Ming
authors.initials: ZM
authors.lastname: Dong
mesh_terms: D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology; D011992:Endosomes / Q000276:immunology*; D005089:Exocytosis / Q000276:immunology*; D006801:Humans; D020014:K562 Cells; D009000:Monocytes / Q000166:cytology; D012334:RNA, Neoplasm / Q000235:genetics* / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014162:Transfection
chemical_list: D012334:RNA, Neoplasm