Molecular typing of isolates of the fish pathogen, Flavobacterium columnare, by single-strand conformation polymorphism analysis.
Flavobacterium columnare intraspecies diversity was revealed by analyzing the 16S rRNA gene and the 16S-23S internal spacer region (ISR). Standard restriction fragment length polymorphism (RFLP) of these sequences was compared with single-strand conformation polymorphism (SSCP). Diversity indexes showed that both 16S-SSCP and ISR-SSCP improved resolution (D>or=0.9) when compared with standard RFLP. ISR-SSCP offered a simpler banding pattern than 16S-SSCP while providing high discrimination between isolates. SSCP analysis of rRNA genes proved to be a simple, rapid, and cost-effective method for routine fingerprinting of F. columnare.
Olivares-Fuster
Oscar
O
Department of Fisheries and Allied Aquacultures, Auburn University, Auburn, AL 36849, USA.
Shoemaker
Craig A
CA
Klesius
Phillip H
PH
Arias
Covadonga R
CR
D000818:Animals; D015373:Bacterial Typing Techniques; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000145:classification; D005399:Fishes / Q000382:microbiology*; D005417:Flavobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000145:classification; D017422:Sequence Analysis, DNA
D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S