Optical and magnetic resonance imaging of cell death and platelet activation using annexin a5-functionalized quantum dots.
A quantum-dot-based nanoparticle is presented, allowing visualization of cell death and activated platelets with fluorescence imaging and MRI. The particle exhibits intense fluorescence and a large MR relaxivity (r1) of 3000-4500 mM-1 s-1 per nanoparticle due to a newly designed construct increasing the gadolinium-DTPA load. The nanoparticle is suitable for both anatomic and subcellular imaging of structures in the vessel wall and is a promising bimodal contrast agent for future in vivo imaging studies.
Prinzen
Lenneke
L
Department of Biophysics, University of Maastricht, and Department of Radiology, University Hospital Maastricht, The Netherlands. L.Prinzen@bf.unimaas.nl
Miserus
Robbert-Jan J H M
RJ
Dirksen
Anouk
A
Hackeng
Tilman M
TM
Deckers
Niko
N
Bitsch
Nicole J
NJ
Megens
Remco T A
RT
Douma
Kim
K
Heemskerk
Johan W
JW
Kooi
M Eline
ME
Frederik
Peter M
PM
Slaaf
Dick W
DW
van Zandvoort
Marc A M J
MA
Reutelingsperger
Chris P M
CP
D017304:Annexin A5 / Q000737:chemistry*; D016923:Cell Death; D020285:Cryoelectron Microscopy; D019786:Gadolinium DTPA; D008279:Magnetic Resonance Imaging / Q000379:methods*; D053758:Nanoparticles; D055095:Optics and Photonics; D015539:Platelet Activation; D011789:Quantum Theory
D017304:Annexin A5; D019786:Gadolinium DTPA