Fast data preprocessing for chromatographic fingerprints of tomato cell wall polysaccharides using chemometric methods.
The variability in the chemistry of cell wall polysaccharides in pericarp tissue of red-ripe tomato fruit (Solanum lycopersicon Mill.) was characterized by chemical methods and enzymatic degradations coupled to high performance anion exchange chromatography (HPAEC) and mass spectrometry analysis. Large fruited line, Levovil (LEV) carrying introgressed chromosome fragments from a cherry tomato line Cervil (CER) on chromosomes 4 (LC4), 9 (LC9), or on chromosomes 1, 2, 4 and 9 (LCX) and containing quantitative trait loci (QTLs) for texture traits, was studied. In order to differentiate cell wall polysaccharide modifications in the tomato fruit collection by multivariate analysis, chromatograms were corrected for baseline drift and shift of the component elution time using an approach derived from image analysis and mathematical morphology. The baseline was first corrected by using a "moving window" approach while the peak-matching method developed was based upon location of peaks as local maxima within a window of a definite size. The fast chromatographic data preprocessing proposed was a prerequisite for the different chemometric treatments, such as variance and principal component analysis applied herein to the analysis. Applied to the tomato collection, the combined enzymatic degradations and HPAEC analyses revealed that the firm LCX and CER genotypes showed a higher proportion of glucuronoxylans and pectic arabinan side chains while the mealy LC9 genotype demonstrated the highest content of pectic galactan side chains. QTLs on tomato chromosomes 1, 2, 4 and 9 contain important genes controlling glucuronoxylan and pectic neutral side chains biosynthesis and/or metabolism.
Quéméner
Bernard
B
INRA, UR Biopolymères, Interactions, Assemblages, rue de la Géraudière, BP 71627, 44316 Nantes Cedex 03, France.
Bertrand
Dominique
D
Marty
Isabelle
I
Causse
Mathilde
M
Lahaye
Marc
M
D019342:Acetic Acid; D002473:Cell Wall / Q000737:chemistry* / Q000201:enzymology; D002852:Chromatography, Ion Exchange; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004951:Esterification; D005819:Genetic Markers; D005838:Genotype; D018551:Solanum lycopersicum / Q000737:chemistry* / Q000201:enzymology; D013058:Mass Spectrometry; D008970:Molecular Weight; D011134:Polysaccharides / Q000032:analysis*; D025341:Principal Component Analysis; D040641:Quantitative Trait Loci; D001616:beta-Galactosidase / Q000378:metabolism
D005819:Genetic Markers; D011134:Polysaccharides; D001616:beta-Galactosidase; D019342:Acetic Acid