[Production of specific CTL induced by exosomes derived from K562 cells].
The aim of this study was to investigate whether exosomes derived from K562 cells and human monocyte-derived dendritic cells (DCs) transfected with total RNA of K562 cells are capable of inducing antigen-specific cytotoxic T lymphocytes (CTL) responses in vitro. DCs were generated from peripheral blood mononuclear cells (PBMNC) of healthy volunteers in the presence of GM-CSF and IL-4, and then were transfected with K562 RNA by using DOTAP lipofection. Exosomes was extracted from the supernatant of DCs and K562 cells. The T cell were activated to be tumor specific CTL after DCs and exosomes were co-cultured with autologous T cells derived from healthy volunteers' PBMNC. The effect of CTL on K562 cells was detected by MTT assay. The results showed that treatment of T cells with exosomes derived from K562 cells or DCs transfected with total RNA of K562 cells could significantly promote their killing ability on K562 cells as compared with untreated T cells (P < 0.05). The killing ability of T cells treated with exosomes on K562 cells was stronger than on HL-60 cells (P < 0.05). It is concluded that the specific CTL immune response to leukemia cells can be induced by exosomes derived from K562 cells.
Chen
Shao-Qian
SQ
Department Hematology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
Du
Ying
Y
Wang
Xin
X
Gu
Qiao-Li
QL
Huang
Yu-Min
YM
Dong
Zi-Ming
ZM
D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology; D011992:Endosomes / Q000276:immunology*; D005089:Exocytosis / Q000276:immunology*; D006801:Humans; D020014:K562 Cells; D009000:Monocytes / Q000166:cytology; D012334:RNA, Neoplasm / Q000235:genetics* / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014162:Transfection
D012334:RNA, Neoplasm