Site-specific labeling of DNA-protein conjugates by means of expressed protein ligation.
Site-specific bioconjugation of protein thioesters with a DNA oligonucleotide was achieved by Expressed Protein Ligation (EPL) and the new thiol group formed upon EPL in the conjugate was selectively coupled with small molecule labels using maleimide chemistry.
Lovrinovic
Marina
M
Universität Dortmund, Fachbereich Chemie, Biologisch-Chemische Mikrostrukturtechnik, Otto-Hahn Str. 6, D-44227 Dortmund, Germany.
Fruk
Ljiljana
L
Schröder
Hendrik
H
Niemeyer
Christof M
CM
D002352:Carrier Proteins / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D007118:Immunoassay; D058619:Maltose-Binding Proteins; D015394:Molecular Structure
D002352:Carrier Proteins; D058619:Maltose-Binding Proteins; D004247:DNA