[Detection of short tandem repeat (STR) polymorphisms by microchip electrophoresis for individual identification of cattle].
A simple and rapid detection of short tandem repeat (STR) markers was studied as a screening test for individual identification of cattle. DNAs were extracted from eight commercial beef samples by a proteinase K-boil method followed by purification with 2-propanol precipitation. Five STR markers, known to be highly polymorphic, were amplified by PCR and analyzed both by a conventional sequencing analysis (SEQ) and by a proposed microchip electrophoresis (MEP). Every marker revealed high polymorphism, such as 5-9 alleles in SEQ analysis, and 4-6 alleles in MEP analysis. This simple and rapid MEP analysis is expected to be an effective screening tool with use of confirmatory SEQ analysis.
Yamaguchi
Akihiro
A
Japan Food Research Laboratories, Chitose, Bunkyo 2-3, Chitose-shi, Hokkaido 066-0052, Japan.
Shimizu
Kaori
K
Mishima
Takashi
T
Hattori
Hideki
H
Katsuda
Shin-ichi
S
Sato
Hidetaka
H
Ueda
Nobuo
N
Sato
Noriyuki
N
D000823:Animal Identification Systems / Q000379:methods*; D000818:Animals; D002417:Cattle / Q000235:genetics*; D004247:DNA / Q000235:genetics; D046209:Electrophoresis, Microchip; D005819:Genetic Markers; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA / Q000379:methods
D005819:Genetic Markers; D004247:DNA