title: Safety and efficacy of cytology brushings versus standard FNA in evaluating cystic lesions of the pancreas: a pilot study.
abstract: BACKGROUND
  Cystic neoplastic lesions of the pancreas (CNLP) are increasingly detected and are associated with a potential for malignant transformation. Diagnostic assessment of these lesions is often limited by the cystic nature and focality of neoplastic progression of these lesions. EUS-guided FNA (EUS-FNA) of cyst fluid and exfoliated cells is one of the most accurate methods of diagnosis but still has limited sensitivity. A new, through-the-needle cytologic brush system has recently been approved for use during EUS evaluation of cystic lesions of the pancreas.
  
  
  OBJECTIVE
  To evaluate the cytologic yield and safety profile of the new cytobrush compared with conventional FNA in evaluating CNLP.
  
  
  METHODS
  Ten consecutive patients with CNLP were included. All cysts were sampled by standard EUS-FNA (0.5 of cyst volume) followed by brush cytology, then by aspiration of the remaining fluid. Fluid samples were separately submitted (standard FNA and cytobrushings FNA) but were read by the same pathologist. Complications were assessed during the immediate postprocedure period (2-3 hours) and by a telephone call conducted approximately 30 days after the procedure to inquire about any new symptoms, including abdominal pain, melena, hematochezia, hematemesis, fever, nausea, and vomiting.
  
  
  METHODS
  High-volume EUS referral center.
  
  
  METHODS
  Ten consecutive patients with CNLP that measured at least 20 mm in maximal dimension were included.
  
  
  METHODS
  Cellularity and presence of diagnostic cells on the FNA.
  
  
  RESULTS
  In 7 of 10 cases, the EchoBrush specimen was superior to FNA in terms of cellularity and detection of diagnostic cells. Two cases had complications: 1 major and 1 minor intracystic bleed. No infection or pancreatitis was observed.
  
  
  CONCLUSIONS
  The interpreting pathologist for the case was not blinded to the results of either of the samples. In addition, this pilot study represents only a single-center experience.
  
  
  CONCLUSIONS
  This study suggests that brush cytology specimens obtained at the time of EUS are superior to conventional FNA because of the higher yield of epithelial cells. It is unclear whether bleeding is more common after EchoBrush sampling; however, caution should be taken in patients who require anticoagulation until further data are available.
authors.affiliation: Division of Gastroenterology and Hepatology, Mayo Clinic College of Medicine, Jacksonville, Florida 32224, USA.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Al-Haddad
authors.forename: Massimo
authors.initials: M
authors.lastname: Raimondo
authors.forename: Timothy
authors.initials: T
authors.lastname: Woodward
authors.forename: Murli
authors.initials: M
authors.lastname: Krishna
authors.forename: Surakit
authors.initials: S
authors.lastname: Pungpapong
authors.forename: Kyung
authors.initials: K
authors.lastname: Noh
authors.forename: Michael B
authors.initials: MB
authors.lastname: Wallace
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D044963:Biopsy, Fine-Needle / Q000379:methods; D020420:Cyst Fluid / Q000166:cytology; D003581:Cytodiagnosis / Q000295:instrumentation / Q000379:methods; D019160:Endosonography; D004867:Equipment Design; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010181:Pancreatic Cyst / Q000000981:diagnostic imaging / Q000473:pathology*; D010190:Pancreatic Neoplasms / Q000000981:diagnostic imaging / Q000473:pathology*; D010865:Pilot Projects; D012449:Safety; D012680:Sensitivity and Specificity; D016896:Treatment Outcome