Bilberry xyloglucan--novel building blocks containing beta-xylose within a complex structure.
Bilberries are known to have one of the most complex xyloglucan structures described in the plant kingdom until now. To characterise this structure, xyloglucans were enzymatically degraded and the oligosaccharides obtained were analysed. More than 20 different building blocks were found to make up the xyloglucan polymer. Bilberry xyloglucan was of XXXG-type, but some XXG-type oligomers were present, as well. The building blocks contain galactose-xylose (L) and fucose-galactose-xylose (F) side chains. In both side chains, the galactose units can be acetylated. In addition, beta-xylose-alpha-xylose (U) side chains were shown. This U chain was present in three building blocks described before (XUXG, XLUG and XUFG) and four novel blocks that have not been described in the literature previously: XUG, XUUG, XLUG and XXUG.
Hilz
Hauke
H
Wageningen University, Department of Agrotechnology and Food Sciences, Laboratory of Food Chemistry, PO Box 8129, 6700 EV Wageningen, The Netherlands.
de Jong
Laura E
LE
Kabel
Mirjam A
MA
Verhoef
René
R
Schols
Henk A
HA
Voragen
Alphons G J
AG
D002240:Carbohydrate Sequence; D019075:Electrophoresis, Capillary; D005936:Glucans / Q000032:analysis / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D029800:Vaccinium myrtillus / Q000737:chemistry*; D014990:Xylans / Q000032:analysis / Q000737:chemistry*; D014994:Xylose / Q000737:chemistry*
D005936:Glucans; D009844:Oligosaccharides; D014990:Xylans; C029353:xyloglucan; D014994:Xylose