Evidence for type III restriction and modification systems in Mycoplasma pulmonis.
Mycoplasma pulmonis possesses a cassette of genes that are predicted to code for type III restriction and modification (R-M) enzymes. Transposon disruption of a gene predicted to code for the endonuclease subunit of the enzyme resulted in loss of R-M activity. Genomic data indicate that the cassette was acquired by horizontal gene transfer and possibly located on a mobile element.
Dybvig
Kevin
K
Department of Genetics, 720 S. 20th St., KAUL 720, University of Alabama at Birmingham, Birmingham, AL 35294-0024, USA. dybvig@uab.edu
Cao
Z
Z
French
C Todd
CT
Yu
Huilan
H
D000818:Animals; D015280:DNA Restriction-Modification Enzymes / Q000235:genetics* / Q000378:metabolism*; D004251:DNA Transposable Elements; D004720:Endonucleases / Q000235:genetics / Q000378:metabolism; D022761:Gene Transfer, Horizontal; D051379:Mice; D016254:Mutagenesis, Insertional; D045803:Mycoplasma pulmonis / Q000201:enzymology* / Q000235:genetics; D016133:Polymerase Chain Reaction; D014169:Transformation, Bacterial
D015280:DNA Restriction-Modification Enzymes; D004251:DNA Transposable Elements; D004720:Endonucleases