[Preparation and preliminary application of monoclonal antibody against alpha-zearalanol].
OBJECTIVE
To prepare monoclonal antibody (mAb) against alpha-zearalanol (alpha-ZER) and develop an immunoassay for the detection of alpha-ZER and its analogues residues in food derived from animal tissues.


METHODS
alpha-ZER was conjugated to BSA as immunogen to immunize BALB/c mice and mAb were prepared by hybridoma technique. mAb's characteristics (titer, Ig subclass, specificity and relative affinity) were identified by ELISA. Standard inhibitive cure was made and sensitivity of the mAb was identified. 37 samples derived from animal liver were detected for alpha-ZER residues by competitive ELISA established in the study.


RESULTS
8 hybridoma cell lines stably secreting anti-alpha-ZER mAb were obtained. The titer of one of them (4E5) was 5.142x10(7). The Ig subclass was IgG1. The mAb was specific for alpha-ZER and its analogues and had no cross-reactivity with other compounds.8 positive results were found from the 37 samples derived from animal liver which were negative detected by HPLC.


CONCLUSIONS
Anti-alpha-ZER mAb has been prepared successfully. A rapid method using the mAb for detecting alpha-ZER and its analogues residues in animal tissues has been established.
Wei
Wan-gui
WG
Shanghai CASB Biotechnology CO., LTD, Chinese Academy of Sciences, Shanghai 200233, China.
Wang
Li
L
Bai
Yun-feng
YF
Gong
Yi
Y
Gu
Ming
M
Zhu
Jian
J
Han
Wei
W
Guan
Rong
R
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity; D002417:Cattle; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology; D008099:Liver / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D015029:Zeranol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000276:immunology
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; C029659:zearalenol; D015029:Zeranol