[Mechanism study of anti-influenza effects of Radix Isatidis water extract by red blood cells capillary electrophoresis].
OBJECTIVE
To research the mechanism of the inhibition effects of BWE on cell attachment of influenza virus by capillary electrophoresis.


METHODS
The morphologic difference of red cells after treating with BWE infected by influenza virus was detected with microscope, capillary electrophoresis and HA.


RESULTS
The pretreatment of the normal cells with BWE inhibited the attachment of influenza to the cells, while no meaningful inhibition was observed when influenza virus was pretreated before being inoculated to cells.


CONCLUSIONS
The results indicate that the inhibition effects of BWE on cell attachment of influenza virus may be an important mechanism of anti-influenza activity of Radix Isatidis Extracts.
Chen
Zhi-Wei
ZW
Institute of Biotechnology, Fuzhou University, Fuzhou 350002, China. zerochen98@163.com
Wu
Ling-Wei
LW
Liu
Shu-Tao
ST
Cai
Chun-Ping
CP
Rao
Ping-Fan
PF
Ke
Li-Jing
LJ
D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D019075:Electrophoresis, Capillary; D004912:Erythrocytes / Q000648:ultrastructure* / Q000821:virology; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D009980:Influenza A virus / Q000187:drug effects*; D031219:Isatis / Q000737:chemistry; D008297:Male; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry
D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal