[Determination of 3 kinds of components in huanglian jiedu decoction by HPLC under different UV wavelengths].
OBJECTIVE
To determinate 3 kinds of effective components (5 compounds) in Huanglian Jiedu decoction at the same time, namely berberine, palmtine, jatrorrhizine, baicalin and geniposide.


METHODS
A HPLC method detected by 3 different UV wavelengths 345 nm for berberine, palmtine and jatrorrhizine, 280 nm for baicalin, 238 nm for geniposide. Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column was used, the mixture of H2O (A) , CH3OH (B) and 0.05% H3PO4 solution (C) as mobile phase in gradient mode. The column temperature was 35 degrees C and the flow rate was 1 mL x min(-1).


RESULTS
This method was applied to determined 5 compounds in Huanglian Jiedu decoction rapidly and accurately.


CONCLUSIONS
The quality control method is convenient, reasonable and credible for Huanglian Jiedu decoction.
Li
Xin-Zhong
XZ
Department of Pharmacy of Xiangya Hospital, Central South University, Changsha 410008, China. xylixin@medmail.com.cn
Lei
Peng
P
Liu
Shao
S
D001599:Berberine / Q000031:analogs & derivatives / Q000032:analysis*; D001600:Berberine Alkaloids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D005419:Flavonoids / Q000032:analysis*; D039823:Iridoids / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry; D011714:Pyrans / Q000032:analysis*; D011786:Quality Control; D014466:Ultraviolet Rays
D001600:Berberine Alkaloids; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D039823:Iridoids; D011714:Pyrans; C045607:huang-lien-chieh-tu-tang; C055785:jatrorrhizine; D001599:Berberine; C007835:geniposide; C038044:baicalin; C005413:palmatine