[An efficient mutational method for photosynthetic bacteria].
The pigment and auxotrophic mutants of Rhodobacter sphaeroides Y6 were obtained by treatment with ethyl methanesulfonate (EMS) followed by lithium chloride (LiCI). Treatment with 0.081 M EPS and subsequent treatment with 0.071 M LiCI resulted in 12% higher frequency of pigment mutations than application of 0.081 M EMS alone; the frequency of auxotrophic mutations increased 2.5-fold when treatment with lithium chloride was applied. A blue shift 10 nm was recorded in the absorption spectrum of carotenoids form YM5-3 green mutant; considerable accumulation of neurosporine was revealed by HPLC and mass spectrometry. The method is efficient for isolating mutants of photosynthetic bacteria.
Lin
J Q
JQ
Xiao
M
M
Long
M T
MT
Han
B
B
Quian
W
W
Du
J
J
D005020:Ethyl Methanesulfonate / Q000494:pharmacology*; D018021:Lithium Chloride / Q000494:pharmacology*; D009154:Mutation; D010788:Photosynthesis; D010860:Pigments, Biological / Q000235:genetics; D012242:Rhodobacter sphaeroides / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism
D010860:Pigments, Biological; D005020:Ethyl Methanesulfonate; D018021:Lithium Chloride