title: Establishment and biological characterization of canine histiocytic sarcoma cell lines.
abstract: Seven novel cell lines from canine histiocytic sarcoma (HS), three of which were disseminated cutaneous HS and four of which were synovial HS, were established. All of the established cell lines had the same morphological (by light and electron microscopic findings), cytochemical (alpha-naphthyl butyrate esterase-positive), and immunohistochemical (vimentin- and lysozyme-positive, and cyto-keratin-negative) characteristics as the original HS tumor cells. All of the established cell lines injected into nude mice subcutaneously produced solid tumors. Because the established cell lines also showed phagocytic and processing activities, the HS tumor cells appear to originate from the mononuclear phagocytic system cells, despite their differences in locations or organs.
authors.affiliation: Department of Veterinary Clinical Pathology, Nippon Veterinary and Life Science University, Musashino-Shi, Tokyo, Japan.
authors.forename: Daigo
authors.initials: D
authors.lastname: Azakami
authors.forename: Makoto
authors.initials: M
authors.lastname: Bonkobara
authors.forename: Tsukimi
authors.initials: T
authors.lastname: Washizu
authors.forename: Akiko
authors.initials: A
authors.lastname: Iida
authors.forename: Mari
authors.initials: M
authors.lastname: Kondo
authors.forename: Rina
authors.initials: R
authors.lastname: Kato
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Niikura
authors.forename: Shuko
authors.initials: S
authors.lastname: Iwaki
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tamahara
authors.forename: Naoaki
authors.initials: N
authors.lastname: Matsuki
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Ono
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D004285:Dogs; D012509:Sarcoma / Q000145:classification / Q000473:pathology / Q000662:veterinary*