Production of D-tagatose at high temperatures using immobilized Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana.
Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana (TNAI) were immobilized in calcium alginate beads. The resulting cell reactor (2.4 U, t (1/2) = 43 days at 70 degrees C) in a continuous recycling mode at 70 degrees C produced 49 and 38 g D-tagatose/l from 180 and 90 g D-galactose/l, respectively, within 12 h.
Hong
Young-Ho
YH
Department of Biotechnology, Yonsei University, Seoul, Korea.
Lee
Dong-Woo
DW
Lee
Sang-Jae
SJ
Choe
Eun-Ah
EA
Kim
Seong-Bo
SB
Lee
Yoon-Hee
YH
Cheigh
Chan-Ick
CI
Pyun
Yu-Ryang
YR
D001089:Arabinose / Q000235:genetics / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D006601:Hexoses / Q000096:biosynthesis* / Q000235:genetics; D007535:Isomerases / Q000235:genetics / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism; D013549:Sweetening Agents / Q000378:metabolism*; D013696:Temperature; D046949:Thermotoga neapolitana / Q000201:enzymology* / Q000235:genetics
D006601:Hexoses; D011994:Recombinant Proteins; D013549:Sweetening Agents; D001089:Arabinose; D007535:Isomerases; C030192:tagatose