title: Prevention of epimerization and quantitative determination of amygdalin in Armeniacae Semen with Schizandrae Fructus solution.
abstract: Armeniacae Semen not only contains amygdalin, but emulsin also, which is an enzyme that hydrolyzes amygdalin. The extraction yield of amygdalin from Armeniacae Semen was low, due to the presence of emulsin, when extracted with water. When Schizandrae Fructus solution was used as the extractant; however, amygdalin was almost completely extracted, regardless of the cutting size, due to the absence of the influence of emulsin. In addition, when the crude powder or small piece forms were used with Schizandrae Fructus solution, on epimerization of the D-amygdalin into neoamygdalin occurred. D-amygdalin and its conversion product, neoamygdalin, were quantitatively analyzed by reverse-phase, high-performance liquid chromatography (HPLC), with an optimized eluent of 10 mM sodium phosphate buffer (pH 2.3), containing 11.5% acetonitrile. The concentration and detector response were linearly correlated over the range 0.05 to 2 mM. The detection limits for both D-amygdalin and neoamygdalin were approximately 5 microM for the amount injected.
authors.affiliation: Department of Oriental Pharmaceutical Sciences, Kyung Hee University, Seoul 130-701, Korea.
authors.forename: Woo-Sang
authors.initials: WS
authors.lastname: Joo
authors.forename: Ji-Seon
authors.initials: JS
authors.lastname: Jeong
authors.forename: Hyogeun
authors.initials: H
authors.lastname: Kim
authors.forename: Yong-Moon
authors.initials: YM
authors.lastname: Lee
authors.forename: Je-Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Seon-Pyo
authors.initials: SP
authors.lastname: Hong
mesh_terms: D000678:Amygdalin / Q000737:chemistry* / Q000302:isolation & purification; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry; D027861:Prunus / Q000737:chemistry*; D032141:Schisandra / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D012996:Solutions; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D014867:Water
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D012996:Solutions; D012997:Solvents; D014867:Water; D000678:Amygdalin