title: Recombinant protein-based assays for detection of antibodies to severe acute respiratory syndrome coronavirus spike and nucleocapsid proteins.
abstract: Recombinant severe acute respiratory syndrome (SARS) nucleocapsid and spike protein-based immunoglobulin G immunoassays were developed and evaluated. Our assays demonstrated high sensitivity and specificity to the SARS coronavirus in sera collected from patients as late as 2 years postonset of symptoms. These assays will be useful not only for routine SARS coronavirus diagnostics but also for epidemiological and antibody kinetic studies.
authors.affiliation: National Centers for Immunization and Respiratory Diseases, Division of Viral Diseases, Respiratory and Gastroenteritis Viruses Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, Mailstop G-18, Atlanta, GA 30333. loh5@cdc.gov
authors.forename: Lia M
authors.initials: LM
authors.lastname: Haynes
authors.forename: Congrong
authors.initials: C
authors.lastname: Miao
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Harcourt
authors.forename: Joel M
authors.initials: JM
authors.lastname: Montgomery
authors.forename: Mai Quynh
authors.initials: MQ
authors.lastname: Le
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Dryga
authors.forename: Kurt I
authors.initials: KI
authors.lastname: Kamrud
authors.forename: Bryan
authors.initials: B
authors.lastname: Rivers
authors.forename: Gregory J
authors.initials: GJ
authors.lastname: Babcock
authors.forename: Jennifer Betts
authors.initials: JB
authors.lastname: Oliver
authors.forename: James A
authors.initials: JA
authors.lastname: Comer
authors.forename: Mary
authors.initials: M
authors.lastname: Reynolds
authors.forename: Timothy M
authors.initials: TM
authors.lastname: Uyeki
authors.forename: Daniel
authors.initials: D
authors.lastname: Bausch
authors.forename: Thomas
authors.initials: T
authors.lastname: Ksiazek
authors.forename: William
authors.initials: W
authors.lastname: Thomas
authors.forename: Harold
authors.initials: H
authors.lastname: Alterson
authors.forename: Jonathan
authors.initials: J
authors.lastname: Smith
authors.forename: Donna M
authors.initials: DM
authors.lastname: Ambrosino
authors.forename: Larry J
authors.initials: LJ
authors.lastname: Anderson
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D000086462:Coronavirus Nucleocapsid Proteins; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008562:Membrane Glycoproteins / Q000276:immunology*; D019590:Nucleocapsid Proteins / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology*; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D045169:Severe Acute Respiratory Syndrome / Q000175:diagnosis*; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000086462:Coronavirus Nucleocapsid Proteins; D008562:Membrane Glycoproteins; D019590:Nucleocapsid Proteins; D011994:Recombinant Proteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; C578557:spike glycoprotein, SARS-CoV