[Study on allergenicity of fresh HAM for type I hypersensitivity].
To investigate whether human amniotic membrane (HAM) preparations have the possibility to type I hypersensitivity and its allergenicity. In systemic active allergic test model, 30 guinea pigs were equally divided into three groups. Each 10 guinea pigs were immunized with fresh HAM homogenate, albumen solution (positive control) and PBS (negative control). After the animals were stimulated with corresponding allergen, observe their reaction till dying or 3 h, then obtain blood samples, to determine blood histamine concentrations using chemical fluorometry and four hemorheologic markers by hemorheology analysis system. The guinea pigs responded to fresh HAM homogenate in almost the same manner as to PBS, and no obvious allergic reaction was observed in the animals except those in positive control group. The blood histamine concentration and four hemorheologic markers showed no significant differences between HAM and PBS (P > 0.05), both were much lower than positive control group (P < 0.01). Fresh HAM won't lead to type I hypersensitivity for lack of allergen performance.
Zhao
Min
M
Department of Ophthalmology, the First Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400016, China. minzhao2002@yahoo.com.cn
Zhang
Qi
Q
Cao
Weixi
W
Teng
Yongzhen
Y
Zhang
Xiaoping
X
Hu
Ke
K
Lu
Jing
J
Qing
Yingxiang
Y
D000485:Allergens / Q000276:immunology*; D000650:Amnion / Q000276:immunology*; D000818:Animals; D006168:Guinea Pigs; D006632:Histamine / Q000097:blood; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology*; D008422:Materials Testing
D000485:Allergens; D006632:Histamine