title: The regulation of stearoyl-CoA desaturase gene expression is tissue specific in chickens.
abstract: Emerging evidence suggests a potential role of stearoyl-CoA desaturase (SCD)-1 in the control of body weight and energy homeostasis. The present study was conducted to investigate the effects of several energy balance-related factors (leptin, cerulenin, food deprivation, genotype, and gender) on SCD gene expression in chickens. In experiment 1, 6-week-old female and male broiler chickens were used. In experiment 2, two groups of 3-week-old broiler chickens were continuously infused with recombinant chicken leptin (8 micro g/kg/h) or vehicle for 6 h. In experiment 3, two groups of 2-week-old broiler chickens received i.v. injections of cerulenin (15 mg/kg) or vehicle. In experiment 4, two broiler chicken lines (fat and lean) were submitted to two nutritional states (food deprivation for 16 or 24 h and feeding ad libitum). At the end of each experiment, tissues were collected for analyzing SCD gene expression. Data from experiment 1 showed that SCD is ubiquitously expressed in chicken tissues with highest levels in the proventriculus followed by the ovary, hypothalamus, kidney, liver, and adipose tissue in female, and hypothalamus, leg muscle, pancreas, liver, and adipose tissue in male. Female chickens exhibited significantly higher SCD mRNA levels in kidney, breast muscle, proventriculus, and intestine than male chickens. However, hypothalamic SCD gene expression was higher in male than in female (P < 0.05). Leptin increased SCD gene expression in chicken liver (P < 0.05), whereas cerulenin decreased SCD mRNA levels in muscle. Both leptin and cerulenin significantly reduced food intake (P < 0.05). Food deprivation for either 16 or 24 h decreased the hepatic SCD gene expression in fat line and lean line chickens compared with their fed counterparts (P < 0.05). The hypothalamic SCD mRNA levels were decreased in both lines only after 24 h of food deprivation (P < 0.05). In conclusion, SCD is ubiquitously expressed in chickens and it is regulated by leptin, cerulenin, nutritional state, and gender in a tissue-specific manner.
authors.affiliation: Laboratory of Physiology, Immunology, and Genetics of Domestic Animals, Catholic University of Leuven, KU Leuven, 3001 Heverlee, Belgium. sami.dridi@biw.kuleuven.be
authors.forename: Sami
authors.initials: S
authors.lastname: Dridi
authors.forename: Mohammed
authors.initials: M
authors.lastname: Taouis
authors.forename: Arieh
authors.initials: A
authors.lastname: Gertler
authors.forename: Eddy
authors.initials: E
authors.lastname: Decuypere
authors.forename: Johan
authors.initials: J
authors.lastname: Buyse
mesh_terms: D000273:Adipose Tissue / Q000033:anatomy & histology; D000818:Animals; D015139:Blotting, Southern / Q000379:methods; D002569:Cerulenin / Q000494:pharmacology; D002645:Chickens / Q000378:metabolism*; D005247:Feeding Behavior; D005260:Female; D005508:Food Deprivation; D015870:Gene Expression / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D005838:Genotype; D007031:Hypothalamus / Q000201:enzymology; D020738:Leptin / Q000494:pharmacology; D008099:Liver / Q000201:enzymology; D008297:Male; D009132:Muscles / Q000201:enzymology; D009752:Nutritional Status; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013230:Stearoyl-CoA Desaturase / Q000032:analysis / Q000235:genetics* / Q000378:metabolism
chemical_list: D020738:Leptin; D012333:RNA, Messenger; D002569:Cerulenin; D013230:Stearoyl-CoA Desaturase