Cytogenetic analysis of human bone marrow-derived mesenchymal stem cells passaged in vitro.
This study concerns the cytogenetic stability of in vitro human bone marrow-derived mesenchymal stem cells (MSCs) in primary culture and after passaging. Bone marrow samples were collected from seven brain malfunction patients involved in autologous MSC transplantation trials. Chromosome preparations from primary MSC cultures and after 3 passages were analyzed by conventional staining and G-banding techniques. All MSCs showed normal diploid karyotypes, 46 XY or 46 XX, without aneuploidy or polyploidy; chromosome structural abnormalities were not detected. The results indicate that the in vitro cultured MSCs retained normal cytogenetics before being transplanted back into the patients.
Zhang
Zhen-Xing
ZX
Department of Neurosurgery, Weifang People's Affiliated Hospital, Weifang Medical College, Weifang 261041, PR China.
Guan
Li-Xue
LX
Zhang
Kun
K
Wang
Shouxian
S
Cao
Pei-Cheng
PC
Wang
Yong-He
YH
Wang
Zhe
Z
Dai
Long-Jun
LJ
D000328:Adult; D001854:Bone Marrow Cells / Q000166:cytology*; D002478:Cells, Cultured; D002675:Child, Preschool; D002871:Chromosome Banding; D002877:Chromosomes, Human / Q000235:genetics; D020732:Cytogenetic Analysis / Q000379:methods*; D005260:Female; D006801:Humans; D007621:Karyotyping; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008677:Metaphase; D008875:Middle Aged