Intimin types determined by heteroduplex mobility assay of intimin gene (eae)-positive Escherichia coli strains.
We developed a quick genetic approach to screen variants of the intimin gene (eae) by using a heteroduplex mobility assay (HMA) that targets the 5' conserved region of eae. The eae variants were categorized into 4 major HMA types and 10 minor subtypes.
Ito
Kenitiro
K
Infectious Diseases Surveillance Center, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama-shi, Tokyo 208-0011, Japan. kito@nih.go.jp
Iida
Mariko
M
Yamazaki
Mitsugu
M
Moriya
Kazuo
K
Moroishi
Sanae
S
Yatsuyanagi
Jun
J
Kurazono
Takayuki
T
Hiruta
Noriaki
N
Ratchtrachenchai
Orn-Anong
OA
D018829:Adhesins, Bacterial / Q000235:genetics* / Q000378:metabolism; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D014644:Genetic Variation; D020180:Heteroduplex Analysis / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012703:Serotyping
D018829:Adhesins, Bacterial; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C073819:eaeA protein, E coli