title: Peptidomic analysis of human peripheral monocytes persistently infected by Chlamydia trachomatis.
abstract: Peptidomic analysis using Differential Peptide Display (DPD) of human peripheral blood mononuclear cells (PBMC) mock-infected or persistently infected by Chlamydia trachomatis (CT) revealed 10 peptides, expressed upon CT infection. Analysis of these 10 candidates by tandem mass spectrometry enabled the determination of seven candidates as fragments from the precursors (I) ferritin heavy chain subunit, (II) HLA class II histocompatibility antigen, (III) vimentin, (IV) indoleamine 2,3-dioxygenase, (V and VI) pre-B cell enhancing factor (PBEF), and (VII) Interleukin-8 (CXCL8). The identified candidates proved the presence of anti-bacterial and immunologically active monocytic proteins after CT infection.
authors.affiliation: Division of Rheumatology, Department of Internal Medicine, Hannover Medical School, Hannover, Germany. krausse-opatz.birgit@mh-hannover.de
authors.forename: Birgit
authors.initials: B
authors.lastname: Krausse-Opatz
authors.forename: Annette
authors.initials: A
authors.lastname: Busmann
authors.forename: Harald
authors.initials: H
authors.lastname: Tammen
authors.forename: Christoph
authors.initials: C
authors.lastname: Menzel
authors.forename: Thomas
authors.initials: T
authors.lastname: Möhring
authors.forename: Nicolas
authors.initials: N
authors.lastname: Le Yondre
authors.forename: Cornelia
authors.initials: C
authors.lastname: Schmidt
authors.forename: Peter
authors.initials: P
authors.lastname: Schulz-Knappe
authors.forename: Henning
authors.initials: H
authors.lastname: Zeidler
authors.forename: Hartmut
authors.initials: H
authors.lastname: Selle
authors.forename: Lars
authors.initials: L
authors.lastname: Köhler
mesh_terms: D002692:Chlamydia trachomatis / Q000276:immunology* / Q000472:pathogenicity / Q000502:physiology; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D006801:Humans; D009000:Monocytes / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology*; D010455:Peptides / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D016207:Cytokines; D010455:Peptides