Role of GPI-anchored NCAM-120 in rabies virus infection.
Although the neural cell adhesion molecule (NCAM) -140 and -180 have been shown to serve as a receptor for rabies virus (RV), it was not known whether the other major isoform of NCAM, GPI-anchored NCAM-120 functions as RV receptor. In this study, we have established HEp-2 cells stably expressing NCAM-120 or NCAM-140, and their susceptibilities to RV infection were compared. The results demonstrated that NCAM-120 served as virus attachment protein; however, the cells expressing NCAM-120 did not support efficient RV replication. Furthermore, the level of IFN-ss mRNA was apparently elevated in NCAM-120 expressing cells but not in NCAM-140 expressing cells, suggesting that GPI-anchored NCAM-120 suppressed RV replication via induction of IFN-ss even though NCAM-120 was able to promote virus penetration into the cells.
Hotta
Kozue
K
The United Graduate school of Veterinary Science, Gifu University, Ynagido 1-1, Gifu 501-1193, Japan.
Motoi
Yurie
Y
Okutani
Akiko
A
Kaku
Yoshihiro
Y
Noguchi
Akira
A
Inoue
Satoshi
S
Yamada
Akio
A
D002460:Cell Line; D005786:Gene Expression Regulation; D006801:Humans; D016899:Interferon-beta / Q000096:biosynthesis / Q000276:immunology; D019006:Neural Cell Adhesion Molecules / Q000502:physiology*; D020033:Protein Isoforms / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D011820:Rabies virus / Q000502:physiology*; D011991:Receptors, Virus / Q000502:physiology*; D053585:Virus Attachment; D014779:Virus Replication
D019006:Neural Cell Adhesion Molecules; D020033:Protein Isoforms; D012333:RNA, Messenger; D011991:Receptors, Virus; D016899:Interferon-beta