Functional proteome analysis of the banana plant (Musa spp.) using de novo sequence analysis of derivatized peptides.
We report the use of chemical derivatization with MALDI-MS/MS analysis for de novo sequence analysis. Using three frequently used homology-based search algorithms, we were able to identify more than 40 proteins from banana, a nonmodel plant with unsequenced genome. Furthermore, this approach allowed the identification of different isoforms. We also observed that the identification score obtained varied according to the position of the peptide sequences in the query using the MS-Blast algorithm.
Samyn
Bart
B
Department of Biochemistry, Physiology and Microbiology, Laboratory of Protein Biochemistry and Protein Engineering, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium. bart.samyn@UGent.be
Sergeant
Kjell
K
Carpentier
Sebastien
S
Debyser
Griet
G
Panis
Bart
B
Swennen
Rony
R
Van Beeumen
Jozef
J
D000465:Algorithms; D000595:Amino Acid Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D018745:Genome, Plant; D005838:Genotype; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D028521:Musa / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D020539:Sequence Analysis, Protein; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014357:Trypsin / Q000494:pharmacology
D010455:Peptides; D010940:Plant Proteins; D020543:Proteome; D014357:Trypsin