title: Metalloproteinases are enriched in microglia compared with leukocytes and they regulate cytokine levels in activated microglia.
abstract: Microglia are resident immune cells within the central nervous system (CNS). They become activated following neurological insults and increase their expression of cytokines. Also elevated in CNS injuries are proteases, including matrix metalloproteinases (MMPs) and A disintegrin and metalloproteinases (ADAMs). The spectrum of metalloproteinase members expressed by microglia and by the systemic leukocytes that infiltrate the injured CNS is unknown, as are their functions. We determined the levels of transcripts encoding all 24 MMPs, nine ADAMs, and their four physiological antagonists, tissue inhibitor of metalloproteinases (TIMPs), in human microglia, B and T cells, monocytes, and neutrophils. We found a distinct pattern for each immune subset and an enrichment of metalloproteinases in microglia compared with leukocytes. When microglia were activated, there was an upregulation of transcripts for nine metalloproteinases, and reduction of TIMP3. Activation of microglia also resulted in increased levels of tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, and IL-10 protein in the conditioned media of cells. The amount of secreted TNF-alpha, but not IL-1beta or IL-10, was suppressed by BB94, a broad spectrum metalloproteinase inhibitor, and by TIMP3 but not TIMP1 or TIMP2. This inhibitory profile suggests the involvement of an ADAM member in TNF-alpha secretion. We conclude that microglia bear a metalloproteinase signature distinct from systemic cells, and that following activation, microglia upregulate TNF-alpha protein levels through a combination of elevated cytokine transcripts, increased metalloproteinase level and activity, and through the decrease of TIMP3. The results have implications for the regulation of neuroinflammation and its outcomes following CNS injuries.
authors.affiliation: School of Biological Sciences, University of East Anglia, Norwich, United Kingdom.
authors.forename: Robert K
authors.initials: RK
authors.lastname: Nuttall
authors.forename: Claudia
authors.initials: C
authors.lastname: Silva
authors.forename: Walter
authors.initials: W
authors.lastname: Hader
authors.forename: Amit
authors.initials: A
authors.lastname: Bar-Or
authors.forename: Kamala D
authors.initials: KD
authors.lastname: Patel
authors.forename: Dylan R
authors.initials: DR
authors.lastname: Edwards
authors.forename: V Wee
authors.initials: VW
authors.lastname: Yong
mesh_terms: D051722:ADAM Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D000328:Adult; D002478:Cells, Cultured; D002490:Central Nervous System / Q000293:injuries; D016207:Cytokines / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007249:Inflammation; D016753:Interleukin-10 / Q000276:immunology / Q000378:metabolism; D053583:Interleukin-1beta / Q000276:immunology / Q000378:metabolism; D007962:Leukocytes / Q000201:enzymology* / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D045726:Metalloproteases / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D017628:Microglia / Q000166:cytology / Q000201:enzymology* / Q000276:immunology; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000378:metabolism; D015854:Up-Regulation / Q000502:physiology
chemical_list: D016207:Cytokines; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D012313:RNA; D045726:Metalloproteases; D051722:ADAM Proteins