title: Preparation of N-acylated proteins modified with fatty acids having a specific chain length using an insect cell-free protein synthesis system.
abstract: To establish a strategy to generate N-acylated proteins modified with fatty acids having a specific chain length, tGelsolin-streptag, an epitope-tagged model protein having an N-myristoylation motif, was synthesized using an insect cell-free protein synthesis system in the presence of acyl-CoA with various fatty acid chain lengths. It was found that the fatty acid species attached to the N-termini fully depended on the acyl-CoA species added to the reaction mixture. N-Acylated proteins with fatty acid chain lengths of 8, 10, 12, and 14 were generated successfully.
authors.affiliation: Life Science Laboratory, Analytical and Measuring Instruments Division, Shimadzu Corporation, Japan. t-suzuki@shimadzu.co.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Masaaki
authors.initials: M
authors.lastname: Ito
authors.forename: Toru
authors.initials: T
authors.lastname: Ezure
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Shikata
authors.forename: Eiji
authors.initials: E
authors.lastname: Ando
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Utsumi
authors.forename: Susumu
authors.initials: S
authors.lastname: Tsunasawa
authors.forename: Osamu
authors.initials: O
authors.lastname: Nishimura
mesh_terms: D000107:Acetylation; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002474:Cell-Free System; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D018411:Spodoptera
chemical_list: D005227:Fatty Acids; D011506:Proteins