title: Activation effects of Allium tuberosum Rottl. on choline acetyltransferase.
abstract: An ethanolic extract of Allium tuberosum Rottl. demonstrated potent choline acetytrasferase (ChAT) activating activity (45%) among 30 screened Korean edible plants. The ChAT activator of A. tuberoum Rottl. was purified and identified as ferulic acid [4-hydroxy-3-methoxycinnamic acid] via silica-gel open-column chromatography, HPLC, EI-MS, and 1H/13C-NMR. The ferulic acid from A. tuberoum Rottl. exhibited ChAT activity in a dose-dependent manner and showed notoxicity in a cell viability assay (MTT assay). We suggest that the ferulic acid from A. tuberoum Rottl. was the strong ChAT activator.
authors.affiliation: Graduate School of Life Sciences and Biotechnology, Korea University, South Korea.
authors.forename: Mi Jeong
authors.initials: MJ
authors.lastname: Kim
authors.forename: Soo Jung
authors.initials: SJ
authors.lastname: Choi
authors.forename: Hye Kyung
authors.initials: HK
authors.lastname: Kim
authors.forename: Chang-Ju
authors.initials: CJ
authors.lastname: Kim
authors.forename: Bumshik
authors.initials: B
authors.lastname: Hong
authors.forename: Young Jun
authors.initials: YJ
authors.lastname: Kim
authors.forename: Dong-Hoon
authors.initials: DH
authors.lastname: Shin
mesh_terms: D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D027782:Chive / Q000737:chemistry*; D002795:Choline O-Acetyltransferase / Q000378:metabolism*; D003373:Coumaric Acids / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology
chemical_list: D003373:Coumaric Acids; D010936:Plant Extracts; C004999:ferulic acid; D002795:Choline O-Acetyltransferase