title: Detection of bovine meat and bone meal in animal feed at a level of 0.1%.
abstract: For the control of the transmission of bovine spongiform encephalopathy in cattle via feedstuff, a real-time polymerase chain reaction assay was developed with ruminant-specific Bov-B SINE primers, SYBR Green fluorescence detection, and melting curve analysis. In formulated cattle and chicken feed samples spiked with pure bovine and sheep meat and bone meal heated at 133 degrees C for 20 min, a contamination level of 0.1% was detected.
authors.affiliation: RIKILT-Institute of Food Safety, Wageningen UR, Bornsesteeg 45, 6708 PD Wageningen, The Netherlands. henk.aarts@wur.nl
authors.forename: Henk J M
authors.initials: HJ
authors.lastname: Aarts
authors.forename: El M
authors.initials: el M
authors.lastname: Bouw
authors.forename: Jaap B
authors.initials: JB
authors.lastname: Buntjer
authors.forename: Johannes A
authors.initials: JA
authors.lastname: Lenstra
authors.forename: Leo W D
authors.initials: LW
authors.lastname: Van Raamsdonk
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D001842:Bone and Bones / Q000737:chemistry*; D002417:Cattle; D002645:Chickens; D004247:DNA / Q000737:chemistry; D017743:Equipment Reuse; D008460:Meat / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012756:Sheep
chemical_list: D004247:DNA