title: [Tissue culture and rapid propagation of Trichosanthes kirilowii].
abstract: OBJECTIVE
  To study tissue culture of Trichosanthes kirilowii, and establish the technique of rapid propagation.
  
  
  METHODS
  Cluster buds were induced from stem tip and stem with axillary bud, calluses were induced from stems and leaves.
  
  
  RESULTS
  Cluster buds could be induced on both the bud and axillary bud from 2-years-old tuber of Trichosanthes kirilowii with MS medium containing 2 mg/L BA and 0.5 -0.05 mg/L NAA. The roots could be induced with MS + 0.1 mg/L NAA + 0.2 mg/L IBA. The seedling with roots could be transplanted after domestication, and achieved the rapid seedling propagation. Calluses could be induced from the stems and leaves with MS + 4 mg/L BA + 0.5 mg/L NAA, and the calluses then could be differentiated into seedlings without root.
  
  
  CONCLUSIONS
  The male and female seedlings of Trichosanthes kirilowii can be propagated largely using stem tip or axillary bud in short period. The technique of rapid propagation on Trichosanthes kirilowii have a high benefit and low costs.
authors.affiliation: School of Life Science and Technology, Tongji University, Shanghai 200092, China.
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Yang
authors.forename: Guan-Rong
authors.initials: GR
authors.lastname: Jin
authors.forename: Duan-Peng
authors.initials: DP
authors.lastname: Yang
authors.forename: Shan
authors.initials: S
authors.lastname: Li
authors.forename: Yun-Guo
authors.initials: YG
authors.lastname: Zhu
mesh_terms: D003470:Culture Media; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000254:growth & development; D018520:Plant Shoots / Q000254:growth & development; D018547:Plant Stems / Q000254:growth & development; D010946:Plants, Medicinal / Q000254:growth & development*; D046509:Tissue Culture Techniques / Q000379:methods*; D029778:Trichosanthes / Q000254:growth & development*
chemical_list: D003470:Culture Media; D010937:Plant Growth Regulators