Analysis of aPKClambda and aPKCzeta reveals multiple and redundant functions during vertebrate retinogenesis.
Retinal lamination is known to depend on cell polarity and localized signaling. In vertebrates, atypical protein kinase C proteins, aPKClambda/iota and aPKCzeta, are essential for apical-basal cell polarity. However, it is not known to what extent functional redundancy has precluded a comprehensive functional characterization of aPKC signaling during vertebrate retinogenesis. Here, we show that aPKCs lambda and zeta are functionally redundant for multiple aspects of retinogenesis including mitotic division location and orientation, cell-type positioning, and retinal pigment epithelial (RPE) and photoreceptor cell morphogenesis. Genetic mosaic analyses demonstrate a cell-autonomous requirement of aPKCs for RPE and photoreceptor development, and a cell-non-cell-autonomous function that is intrinsic to the neural retina for cell-type positioning. Our observations uncover a previously unappreciated involvement of aPKCzeta during zebrafish retinogenesis and suggest that aPKC signaling primes the retinal environment for appropriate cell migration of post-mitotic progenitor cells but is not essential for correct cell-type specification.
Cui
Shuang
S
Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
Otten
Cécile
C
Rohr
Stefan
S
Abdelilah-Seyfried
Salim
S
Link
Brian A
BA
D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002452:Cell Count; D002454:Cell Differentiation / Q000502:physiology; D016764:Cell Polarity / Q000502:physiology; D049109:Cell Proliferation; D004625:Embryo, Nonmammalian; D005136:Eye Proteins / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000032:analysis / Q000502:physiology*; D009024:Morphogenesis / Q000502:physiology*; D011493:Protein Kinase C / Q000032:analysis / Q000502:physiology*; D012160:Retina / Q000166:cytology* / Q000196:embryology; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism; D000099293:Protein Kinase C-lambda; D000099221:Protein Kinase C zeta
D001973:Bromodeoxyuridine; D005136:Eye Proteins; D007527:Isoenzymes; D011493:Protein Kinase C; D029961:Zebrafish Proteins; D000099293:Protein Kinase C-lambda; D000099221:Protein Kinase C zeta; C420034:epb41l5 protein, zebrafish