title: Use of Fourier transform infrared microscopy for the evaluation of drug efficiency.
abstract: Fourier transform infrared (FTIR) spectroscopy has been used by chemists as a powerful tool to characterize inorganic and organic compounds. In this study, we examine the potential of FTIR microspectroscopy for early evaluation of therapy efficiency. For this purpose, we examine the effect of acyclovir (a known antiherpetic drug) on the development of herpes simplex virus type 1 (HSV-1) infection in cell culture. Also, we examine spectral changes in lymphocytes obtained from leukemia patients after appropriate chemotherapy treatment. Our results show early and significant spectral indicators for successful infection of Vero cells with HSV-1. Treatment of these infected cells with increasing doses of acyclovir reduces clearly the spectral changes caused by the infection in a correlation with inhibiting the development of the cytopathic effect (CPE) induced by this virus. Also significant and consistent spectral differences between lymphocytes from human leukemia patients compared to that from healthy persons are obtained. Treatment of these leukemia patients with appropriate drugs reduces significantly these spectral differences in a correlation with the improvement of the patient's clinical situation. It seems that FTIR spectroscopy can be used as an effective tool for early evaluation of the efficiency of drugs.
authors.affiliation: Department of Virology, Ben Gurion University of the Negev, The National Institute for Biotechnology, Beer-Sheva, Israel.
authors.forename: Vitaly
authors.initials: V
authors.lastname: Erukhimovitch
authors.forename: Marina
authors.initials: M
authors.lastname: Talyshinsky
authors.forename: Yelena
authors.initials: Y
authors.lastname: Souprun
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Huleihel
mesh_terms: D000212:Acyclovir / Q000008:administration & dosage*; D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage; D002522:Chlorocebus aethiops; D016000:Cluster Analysis; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000502:physiology*; D006801:Humans; D007668:Kidney / Q000187:drug effects* / Q000382:microbiology*; D008853:Microscopy / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013057:Spectrum Analysis / Q000379:methods; D014709:Vero Cells
chemical_list: D000998:Antiviral Agents; D000212:Acyclovir