Attenuated vasoconstrictor responses to endothelin in afferent arterioles during a high-salt diet.
Endothelin-1 (ET-1) is increased in rats on a high-salt (HS) diet and participates in salt-dependent hypertension. Afferent arterioles (AA) are important for long-term blood pressure control, and therefore we hypothesized that a HS diet would alter their responsiveness to ET-1. Sprague-Dawley rats were fed either a normal-salt (NS; 0.66% NaCl) or HS (8%) diet for 1 wk. Diameters of AA were determined in response to increasing concentrations of big ET-1, ET-1, sarafotoxin 6c (S6c), or norepinephrine (NE), using the blood-perfused juxtamedullary nephron technique. ET-1 responses were also determined during blockade of endothelin type A (ET(A)) or type B (ET(B)) receptors with 10 nM ABT-627 or 30 nM A-192621, respectively. Expression of ET(A) and ET(B) receptors was determined in renal microvessels. Responses of AA to big ET-1, ET-1, and S6c were significantly attenuated during a HS diet (e.g., response to 10(-10) M ET-1 in NS vs. HS rats: -52.5 +/- 10.2 vs. +5.6 +/- 11.3% of control diameter; P < 0.05), with no change in the responses to NE. ET(B), but not ET(A) receptor blockade abolished the different response to ET-1 between the NS and HS groups. ET(B) receptor expression in renal microvessels was increased in response to HS (17.7 +/- 2.4 vs. 6.6 +/- 3.0% of beta-actin, P = 0.02), whereas ET(A) receptor expression was unchanged. These results suggest that the reduced vasoconstrictor response of AA to endothelin peptides during a HS diet is mediated by increased vasodilatory function of endothelial ET(B) receptors. By preserving renal blood flow, this may be an important mechanism to restore sodium balance during a HS diet.
Schneider
Markus P
MP
Vascular Biology Center, Medical College of Georgia, 1459 Laney Walker Blvd., Augusta, GA 30912-2500, USA.
Inscho
Edward W
EW
Pollock
David M
DM
D000818:Animals; D001160:Arterioles / Q000187:drug effects / Q000502:physiology*; D001794:Blood Pressure / Q000187:drug effects; D019332:Endothelin-1 / Q000502:physiology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D044022:Receptor, Endothelin A / Q000096:biosynthesis; D044023:Receptor, Endothelin B / Q000096:biosynthesis; D012982:Sodium, Dietary / Q000008:administration & dosage*; D014661:Vasoconstriction / Q000187:drug effects*
D019332:Endothelin-1; D044022:Receptor, Endothelin A; D044023:Receptor, Endothelin B; D012982:Sodium, Dietary