[Fluorescence spectra and test application study on the interaction of fluorescein and bovine serum albumin].
A fluorescence method is found for determination of bovine serum albumin (BSA). The method is based on the interaction of fluorescein with BSA to form a complex in Tris-HCl buffer of pH 2.2. The complex exitation wavelength is 467 nm, the emission wavelength is 515 nm. The linear measurement rang is between 1.8-500 mg/L for BSA, F = 26.776 C + 2.8082, r = 0.9999. This method is sensitive,steady,and low cost for determination of BSA.
Huang
Xun
X
Department of Biomedical Analysis, Analysis and Testing Center, Sichuan University, Chengdu 610041, China. huangfxcz@163.com
Sun
Ai'ming
A
Ding
Xueqin
X
Xu
Xiucheng
X
Fang
Luyan
L
D000818:Animals; D002417:Cattle; D004347:Drug Interactions; D019793:Fluorescein / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods
D012710:Serum Albumin, Bovine; D019793:Fluorescein