title: Mitoproteome plasticity of rat brown adipocytes in response to cold acclimation.
abstract: Cold acclimation induces an adaptative increase in respiration in brown adipose tissue (BAT). A comparative analysis by two-dimensional differential in-gel electrophoresis of mitochondrial protein patterns found in rat control and cold-acclimated BAT was performed. A total of 58 proteins exhibiting significant differences in their abundance was unambiguously identified. Proteins implicated in the major catabolic pathways were up-regulated as were ATP synthase and mitofilin. Moreover, these results support the fact that adipocytes can balance their ATP synthesis and their heat production linked to UCP1-sustained uncoupling.
authors.affiliation: Laboratory of Bioenergetics, and Centre de Recherche en Neurobiologie Cellulaire et Moléculaire, Liège, Belgium.
authors.forename: Rachel
authors.initials: R
authors.lastname: Navet
authors.forename: Gregory
authors.initials: G
authors.lastname: Mathy
authors.forename: Pierre
authors.initials: P
authors.lastname: Douette
authors.forename: Rowan Laura
authors.initials: RL
authors.lastname: Dobson
authors.forename: Pierre
authors.initials: P
authors.lastname: Leprince
authors.forename: Edwin
authors.initials: E
authors.lastname: De Pauw
authors.forename: Claudine
authors.initials: C
authors.lastname: Sluse-Goffart
authors.forename: Francis E
authors.initials: FE
authors.lastname: Sluse
mesh_terms: D025181:ATP Synthetase Complexes / Q000096:biosynthesis; D000064:Acclimatization; D017667:Adipocytes / Q000378:metabolism*; D002001:Adipose Tissue, Brown / Q000166:cytology*; D000818:Animals; D001833:Body Temperature Regulation; D003080:Cold Temperature; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D007473:Ion Channels / Q000096:biosynthesis; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000096:biosynthesis; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D000071256:Uncoupling Protein 1
chemical_list: D007473:Ion Channels; D024101:Mitochondrial Proteins; C000604373:Ucp1 protein, rat; D000071256:Uncoupling Protein 1; D025181:ATP Synthetase Complexes