Separation of solanesol in tobacco leaves extract by slow rotary counter-current chromatography using a novel non-aqueous two-phase solvent system.
Non-aqueous solvent system composed of sunflower oil-ethanol was found to be suitable for the separation of lower polarity components. Employing this solvent system, 3 g of tobacco leaves extract containing 15% solanesol was separated using a slow rotary counter-current chromatograph equipped with an 1100 mL column made of 5.7 mm I.D. convoluted PTFE tube. The separation yielded 1.5 g of yellow oily product in which solanesol occupied 26.8% determined by HPLC. The tobacco tar and other main impurities in the tobacco leaves extract were removed after slow rotary counter-current chromatographic separation. The separation processing may be developed as a technique for producing a solanesol product with food grade.
Zhao
Yang
Y
Institute of Food and Biological Engineering, Zhejiang Gongshang University, Hangzhou 310035, China.
Du
Qizhen
Q
D002855:Chromatography, Thin Layer; D003377:Countercurrent Distribution / Q000379:methods*; D018515:Plant Leaves / Q000737:chemistry*; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry*; D013729:Terpenes / Q000737:chemistry / Q000302:isolation & purification*; D014026:Nicotiana / Q000737:chemistry*
D012997:Solvents; D013729:Terpenes; C017719:solanesol