title: Colonization capacity and serum bactericidal activity of Haemophilus parasuis thy mutants.
abstract: The bacterial thyA gene encodes the enzyme thymidylate synthase, which is essential for dTMP synthesis and, consequently, for DNA replication. In this work, a Haemophilus parasuis thyA mutant was constructed in order to analyze its colonization characteristics and its capacity to generate serum bactericidal activity in infected guinea pigs. The data showed that colonization by the H. parasuis thyA mutant was much less than that of the wild-type strain. Nevertheless, the mutant generated a strong immunogenic response in the host, as detected by measuring serum bactericidal activity.
authors.affiliation: Department of Genetics and Microbiology, Autonomous University of Barcelona, Bellaterra, Spain.
authors.forename: Anna
authors.initials: A
authors.lastname: Bigas
authors.forename: M Elena
authors.initials: ME
authors.lastname: Garrido
authors.forename: Ignacio
authors.initials: I
authors.lastname: Badiola
authors.forename: Jordi
authors.initials: J
authors.lastname: Barbé
authors.forename: Montserrat
authors.initials: M
authors.lastname: Llagostera
mesh_terms: D000818:Animals; D001770:Blood Bactericidal Activity; D006168:Guinea Pigs; D006192:Haemophilus Infections / Q000097:blood / Q000276:immunology* / Q000382:microbiology; D044137:Haemophilus parasuis / Q000235:genetics / Q000254:growth & development / Q000276:immunology / Q000502:physiology*; D013940:Thymidylate Synthase / Q000235:genetics / Q000378:metabolism
chemical_list: D013940:Thymidylate Synthase