HSP90-like artificial chaperone activity based on indole beta-cyclodextrin.
Indole beta-cyclodextrin (beta-1) was found to be able to prevent aggregation of citrate synthase (CS) on heating condition. As a result, beta-1 showed anti-CS aggregation in this system by regulating in early stage. The depression mechanism of beta-1 for aggregation of CS is as follows: the beta-1 formed a complex with hydrophobic parts of the beta-sheet structure of CS. From CD spectra, CS was changed own conformation was changed by beta-1 addition. So, it was concluded that beta-1 works as beta-sheet inducer in thermal condition. On the other hand, native beta-cyclodextrin (beta-CyD) shows small suppression capability for CS aggregation.
Toda
Masaya
M
Department of Material-Process Engineering and Applied Chemistry for Environments, Faculty of Engineering and Resource Science, Akita University, Tegata, Akita 010-8502, Japan.
Itoh
Hideaki
H
Kondo
Yoshihiko
Y
Hamada
Fumio
F
D002942:Circular Dichroism; D018841:HSP90 Heat-Shock Proteins / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D013056:Spectrophotometry, Ultraviolet; D047392:beta-Cyclodextrins / Q000737:chemistry*
D018841:HSP90 Heat-Shock Proteins; D007211:Indoles; D047392:beta-Cyclodextrins; C031215:betadex