Transformation of Progesterone by Traemetes hispida.
TRAEMETES HISPIDA was found to convert progesterone into a mixture of various products. Five products were isolated in sufficient amounts by HPLC analysis for structural identification and characterization. Three products were found to have two hydroxy moieties on axial and equatorial positions at 7beta,15beta, 6beta, 11alpha, and 11alpha, 15beta sites. The other two products had an extra 17alpha-hydroxy on the above dihydroxy derivatives to give trihydroxyprogesterone. The hydroxylating steps caused by this strain were greatly influenced by the 17beta side chain, especially by the C-20 moiety in the steroidal skeleton.
Dahiya
J S
JS
Department of Plant Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.