title: [Comparison of minimum inhibitory concentration and postantibiotic effect of eyedrops on isolates in National Surveillance of Infectious Keratitis in Japan].
abstract: OBJECTIVE
  To determine the minimum inhibitory concentration (MIC) and postantibiotic effect (PAE) of antibiotic eyedrops against various isolates from infectious keratitis samples.
  
  
  METHODS
  MIC and postantibiotic bactericidal effect (PABE)/postantibiotic fungicidal effect (PAFE) after 4 min of exposure to nine antibacterial eyedrops or four antifungals were examined using 100 clinical isolates collected by the National Surveillance of Infectious Keratitis in Japan in 2003.
  
  
  RESULTS
  The PABEs of tobramycin and micronomicin sulfate showed better results than other drugs, and the other drugs demonstrated various PABEs depending on the bacterial strains; in particular most drugs showed low PABE against Serratia marcescens. There were no significant differences between PABEs of methicillin-resistant and methicillin-susceptible Staphylococci. Also there was no statistical correlation between MIC and PABE of Staphylococci. All the antifungals demonstrated low PAFE against Candida spp.
  
  
  CONCLUSIONS
  PABE/PAFEs vary among species of microorganisms, and since they have no relation with the usual MIC, various other factors, including species of isolates, actual clinical effects, and prevention of drug-resistance induction, should be considered in the selection of drugs.
authors.affiliation: Department of Medical Technology, Osaka University Hospital. 2-15 Yamadaoka, Suita 565 0871, Japan. acchan@hp-lab.med.osaka-u.ac.jp
authors.forename: Atsuko
authors.initials: A
authors.lastname: Sunada
authors.forename: Akiko
authors.initials: A
authors.lastname: Ueda
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Inoue
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Ohashi
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Uno
authors.forename: Kazuko
authors.initials: K
authors.lastname: Kitagawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hatano
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shiota
authors.forename: Seishi
authors.initials: S
authors.lastname: Asari
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects* / Q000302:isolation & purification*; D001424:Bacterial Infections; D002175:Candida / Q000187:drug effects* / Q000302:isolation & purification*; D024881:Drug Resistance, Bacterial; D025141:Drug Resistance, Fungal; D015821:Eye Infections, Fungal; D006801:Humans; D007564:Japan; D007634:Keratitis / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D009883:Ophthalmic Solutions; D011358:Product Surveillance, Postmarketing
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D009883:Ophthalmic Solutions