Counting colonies of clonogenic assays by using densitometric software.
Clonogenic assays are a useful tool to test whether a given cancer therapy can reduce the clonogenic survival of tumour cells. A colony is defined as a cluster of at least 50 cells which can often only be determined microscopically. The process of counting colonies is very extensive work and so we developed software that is able to count the colonies automatically from scanned flasks. This software is made freely available by us with a detailed description how to use and install the necessary features.
Niyazi
Maximilian
M
CCC Tübingen, Department of Radiation Oncology, Hoppe-Seyler-Str, 3, 72076 Tübingen, Germany. maxi@niyazi.de
Niyazi
Ismat
I
Belka
Claus
C
D000465:Algorithms; D001331:Automation; D001681:Biological Assay / Q000379:methods; D035843:Biomedical Research / Q000295:instrumentation / Q000639:trends; D002452:Cell Count / Q000379:methods*; D045744:Cell Line, Tumor; D002470:Cell Survival; D003196:Computer Graphics; D003720:Densitometry / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D011381:Programming Languages; D015203:Reproducibility of Results; D012984:Software