Investigating membrane protein dynamics in living cells.
Live cell imaging is a powerful tool for understanding the function and regulation of membrane proteins. In this review, we briefly discuss 4 fluorescence-microscopy-based techniques for studying the transport dynamics of membrane proteins: fluorescence-correlation spectroscopy, image-correlation spectroscopy, fluorescence recovery after photobleaching, and single-particle and (or) molecule tracking. The advantages and limitations of each approach are illustrated using recent studies of an ion channel and cell adhesion molecules.
Bates
Ian R
IR
Department of Physiology, McGill University, 3655 Promenade Sir William Osler, Montréal, QC H3G 1Y6, Canada. ian.bates@mcgill.ca
Wiseman
Paul W
PW
Hanrahan
John W
JW
D000818:Animals; D002462:Cell Membrane / Q000737:chemistry*; D002477:Cells / Q000737:chemistry / Q000378:metabolism*; D036681:Fluorescence Recovery After Photobleaching; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis*; D038761:Photobleaching; D013050:Spectrometry, Fluorescence
D008565:Membrane Proteins