The levels of PCV2 specific antibodies and viremia in pigs.
Porcine circovirus 2 quantification by real-time PCR and determination of virus specific antibodies using a peptide based ELISA test was performed on a total of 400 serum samples. Samples for antibody measurement and virus quantification were obtained from a conventional pig farm with a clinical form of post-weaning multisystemic wasting syndrome. Samples were taken during the post-weaning period. Seven litters of weaned piglets (6-25 weeks) were systematically sampled at 2-3 week intervals. Porcine circovirus 2 specific antibodies were detected using the peptide based ELISA test. IgM antibodies were first detected at week 8 and reached their highest levels at week 12; IgG antibody appeared at week 10 and peaked at week 16 with an average titer at 1:3500. Although, the viral load peaked at week 10 (7x10(7) genomes copy/ml of sera), viral infection persisted through to adult age (10(5) genomes copy/ml of sera).
Carasova
P
P
Institute of Microbiology and Immunology, Faculty of Veterinary Medicine, Veterinary and Pharmaceutical University Brno, Palackeho 1/3, 612 24 Brno, Czech Republic.
Celer
V
V
Takacova
K
K
Trundova
M
M
Molinkova
D
D
Lobova
D
D
Smola
J
J
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D018173:Circoviridae Infections / Q000097:blood / Q000276:immunology / Q000662:veterinary*; D017925:Circovirus / Q000276:immunology*; D005260:Female; D013552:Swine; D013553:Swine Diseases / Q000097:blood* / Q000276:immunology / Q000821:virology; D014766:Viremia
D000914:Antibodies, Viral