title: Differential transmission of actin motion within focal adhesions.
abstract: Cell migration requires the transmission of motion generated in the actin cytoskeleton to the extracellular environment through a complex assembly of proteins in focal adhesions. We developed correlational fluorescent speckle microscopy to measure the coupling of focal-adhesion proteins to actin filaments. Different classes of focal-adhesion structural and regulatory molecules exhibited varying degrees of correlated motions with actin filaments, indicating hierarchical transmission of actin motion through focal adhesions. Interactions between vinculin, talin, and actin filaments appear to constitute a slippage interface between the cytoskeleton and integrins, generating a molecular clutch that is regulated during the morphodynamic transitions of cell migration.
authors.affiliation: Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
authors.forename: Ke
authors.initials: K
authors.lastname: Hu
authors.forename: Lin
authors.initials: L
authors.lastname: Ji
authors.forename: Kathryn T
authors.initials: KT
authors.lastname: Applegate
authors.forename: Gaudenz
authors.initials: G
authors.lastname: Danuser
authors.forename: Clare M
authors.initials: CM
authors.lastname: Waterman-Storer
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D000185:Actinin / Q000378:metabolism; D000199:Actins / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002465:Cell Movement; D005109:Extracellular Matrix / Q000378:metabolism; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D022001:Focal Adhesions / Q000378:metabolism*; D039302:Integrin alphaVbeta3 / Q000378:metabolism; D008840:Microfilament Proteins / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D009010:Monte Carlo Method; D051419:Paxillin / Q000378:metabolism; D048328:Potoroidae; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D016608:Talin / Q000378:metabolism; D016596:Vinculin / Q000378:metabolism
chemical_list: D000199:Actins; D039302:Integrin alphaVbeta3; D008840:Microfilament Proteins; D051419:Paxillin; D011993:Recombinant Fusion Proteins; D016608:Talin; D000185:Actinin; D016596:Vinculin; D051416:Focal Adhesion Protein-Tyrosine Kinases