Shedding off specific lipid constituents from sperm cell membrane during cryopreservation.
Membrane damage is one of the main reasons for reduced motility and fertility of sperm cells during cryopreservation. Using a model system of sperm cryopreservation developed in our laboratory, we have investigated the detailed changes due to cryopreservation in the plasma membrane lipid composition of the goat epididymal sperm cells. Total lipid and its components, i.e., neutral lipids, glycolipids and phospholipids decreased significantly after cryopreservation. Among neutral lipids sterols, steryl esters and 1-O-alkyl-2,3-diacyl glycerols decreased appreciably, while among phospholipids, major loss was observed for phosphatidyl choline and phosphatidyl ethanolamine. Unsaturated fatty acids bound to the phospholipids diminished while the percentage of saturated acids increased. The cholesterol:phospholipid ratio enhanced and the amount of hydrocarbon, which was unusually high, increased further on cryopreservation. The data indicates that profound increase of the hydrophobicity of the cell membrane is one of the major mechanisms by which spermatozoa acquire potential to resist or combat stress factors like cryodamage. The results are compatible with the view that for survival against cryodamage, sperm cells modulate the structure of their outer membrane by shedding off preferentially some hydrophilic lipid constituents of the cell membrane.
Chakrabarty
Jitamanyu
J
Sperm Biology Division, Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, Jadavpur, Kolkata 700 032, India.
Banerjee
Dipankar
D
Pal
Debasish
D
De
Joydeep
J
Ghosh
Amitabha
A
Majumder
G C
GC
D000818:Animals; D002462:Cell Membrane / Q000737:chemistry*; D015925:Cryopreservation / Q000379:methods*; D004822:Epididymis / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis; D006041:Goats / Q000378:metabolism; D057927:Hydrophobic and Hydrophilic Interactions; D008297:Male; D008563:Membrane Lipids / Q000737:chemistry*; D010743:Phospholipids / Q000032:analysis; D012662:Semen Preservation / Q000379:methods*; D013094:Spermatozoa / Q000737:chemistry*; D013261:Sterols / Q000032:analysis
D005227:Fatty Acids; D008563:Membrane Lipids; D010743:Phospholipids; D013261:Sterols