title: Structural characterization of a glucan isolated from Hedyotis diffusa Willd.
abstract: OBJECTIVE
  To isolate a homogeneous polysaccharide RP from RDP (a crude polysaccharide from Dioscorea opposita Thunb.), and study its preliminary com-position and structure.
  
  
  METHODS
  RP was obtained with water extraction, alcohol sedimentation, CTAB deprotein, cellulose column and SephadexG-100 column. The purities of RP were identified by SephadexG-200. PC analysis on its acidic hydrolysates was used to determine the sugar components. Sephadex chromatography was used to mensurate its molecular weight. IR was used to analysis RP.
  
  
  RESULTS
  RP was homogeneous. IR indicated that RP had P configurations. The compositon was identified by paper chromatography as glucose, D-mannose, D-galactose.
  
  
  CONCLUSIONS
  The research could provide a theoretical foundation for further development and utilization.
authors.affiliation: Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Cui
authors.forename: Shun-chun
authors.initials: SC
authors.lastname: Wang
authors.forename: Song-shan
authors.initials: SS
authors.lastname: Shi
authors.forename: Zheng-tao
authors.initials: ZT
authors.lastname: Wang
mesh_terms: D005936:Glucans / Q000737:chemistry* / Q000302:isolation & purification*; D005947:Glucose / Q000737:chemistry; D032064:Hedyotis / Q000737:chemistry*; D015394:Molecular Structure; D008970:Molecular Weight; D010946:Plants, Medicinal / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D005936:Glucans; D005947:Glucose