Transcriptional analysis of L-methionine catabolism in Brevibacterium linens ATCC9175.
The expression of genes possibly involved in L-methionine and lactate catabolic pathways were performed in Brevibacterium linens (ATCC9175) in the presence or absence of added L-methionine. The expression of 27 genes of 39 selected genes differed significantly in L-methionine-enriched cultures. The expression of the gene encoding L-methionine gamma-lyase (MGL) is high in L-methionine-enriched cultures and is accompanied by a dramatic increase in volatile sulfur compounds (VSC) biosynthesis. Several genes encoding alpha-ketoacid dehydrogenase and one gene encoding an acetolactate synthase were also up-regulated by L-methionine, and are probably involved in the catabolism of alpha-ketobutyrate, the primary degradation product of L-methionine to methanethiol. Gene expression profiles together with biochemical data were used to propose catabolic pathways for L-methionine in B. linens and their possible regulation by L-methionine.
Cholet
Orianne
O
Institut National de la Recherche Agronomique, UMR Génie et Microbiologie des Procédés Alimentaires, CBAI, 78850 Thiverval-Grignon, France.
Hénaut
Alain
A
Bonnarme
Pascal
P
D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide) / Q000235:genetics / Q000378:metabolism; D000095:Acetolactate Synthase / Q000235:genetics / Q000378:metabolism; D001951:Brevibacterium / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D013437:Carbon-Sulfur Lyases / Q000235:genetics / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D008715:Methionine / Q000378:metabolism / Q000494:pharmacology*; D008954:Models, Biological; D020411:Oligonucleotide Array Sequence Analysis; D013457:Sulfur Compounds / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects*
D013457:Sulfur Compounds; D008715:Methionine; D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide); D000095:Acetolactate Synthase; D013437:Carbon-Sulfur Lyases; C021558:L-methionine gamma-lyase