title: [Isolation of helicobacter pylori in dental plaque in patients with gastritis at "Angamos" clinic].
abstract: OBJECTIVE
  Detect the presence of (Helicobacter pylori) in dental plaque among patients with gastritis diagnosed by biopsy at Angamos Surquillo Hospital.
  
  
  METHODS
  
  
  
  METHODS
  Analytical Observation.
  
  
  METHODS
  115 patients scheduled for endoscopy at the Gastroenterological Department of said hospital. The methodology employed for the biopsy of the antrum and fundus of the stomach was by means of a gastroscope. Once the sample was collected it was processed and stained with hematoxilin-eosin for the histological diagnosis of gastritis by observation of Helicobacter pylori. Dental plaque samples were obtained by scraping the supragingival area and were transported in a conservation medium for culture of the bacteria in a selective medium and incubated in microaerophilia for 5 to 10 days;suspected colonies were stained and compared to a patron strain. Urease and oxidase tests confirmed the diagnosis.
  
  
  RESULTS
  In total, 66 cases of gastritis associated with H.p. were found by biopsy. In 4 of these cases, H.p from dental plaque were also isolated. In another group of 49 fases of gastritis not associated with H.p., no H.p. was found in the dental plaque of 45 of these cases while H.p. was isolated from dental plaque in 4 clases.
  
  
  CONCLUSIONS
  The results show a direct relation between the cases of gastritis and the finding of Helicobacter pylori in dental plaque, which would indicate a relation between the patients and the bacteria in these cases, as they act as a reservoir for said bacteria; as compared with the cases in which there is no H.p presence in gastritis without Helicobacter, except in four cases. The cases of gastritis with Helicobacter pylori and negative dental plaque are not significant, in these cases the bacteria entered with the food.
authors.affiliation: Instituto de Medicina Tropical Daniel A Carrión, Facultad de Medicina, UNMSM.
authors.forename: J
authors.initials: J
authors.lastname: Chumpitaz Conde
authors.forename: J
authors.initials: J
authors.lastname: Gutiérrez Manay
authors.forename: R
authors.initials: R
authors.lastname: Córdova Acosta
authors.forename: M
authors.initials: M
authors.lastname: Sánchez Medina
authors.forename: N
authors.initials: N
authors.lastname: Vásquez Valverde
authors.forename: C
authors.initials: C
authors.lastname: Rivadeira Malaga
authors.forename: O
authors.initials: O
authors.lastname: Beteta Del Carpio
authors.forename: L
authors.initials: L
authors.lastname: Solano Mendoza
authors.forename: L
authors.initials: L
authors.lastname: Marocho Chahuayo
authors.forename: E
authors.initials: E
authors.lastname: Pareja Cuadros
authors.forename: A
authors.initials: A
authors.lastname: Huaman Reyes
authors.forename: E
authors.initials: E
authors.lastname: Valencia Bazalar
mesh_terms: D000328:Adult; D000368:Aged; D003773:Dental Plaque / Q000382:microbiology*; D005260:Female; D005756:Gastritis / Q000382:microbiology*; D016481:Helicobacter Infections / Q000453:epidemiology / Q000382:microbiology; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010568:Peru / Q000453:epidemiology