Physico-chemical characterization and biocompatibility evaluation of hydroxyapatites.
The aim of this study was to evaluate the physico-chemical and biocompatibility characteristics of two different hydroxyapatites. Physical and chemical properties were analyzed using granulometric analysis, scanning electron microscopy (SEM), X-ray energy-dispersion (EDX), X-ray fuorescence (XRF) and X-ray diffraction (XRD). Biomaterials were implanted into the subcutaneous tissue on the dorsum of 36 Wistar rats, divided into the following groups: Group 1 - Gen-Ox (natural); Group 2 - HA-U (synthetic) and Group 3 - Control (Sham). After 15 and 30 days, 6 animals/period were sacrificed and the subcutaneous tissue was taken for histological and histometric analysis, giving consideration to inflammatory reaction and granule area. The granulometric test results showed a mean granule diameter of 161.6 microm (min = 19.0 microm; max = 498.0 microm) and 48.7 microm (min = 7.0 microm; max = 256.0 microm) for groups 1 and 2 respectively. Analysis with SEM demonstrated irregular and sharp-edge particles in group 1 (3332.8 +/- 274.3 microm(2)) and irregular and rounded particles in group 2 (1320.8 +/- 83.0 microm(2)) (P < 0.0001; Student's t test). EDX and XRF revealed calcium, carbon, oxygen, sodium and phosphorus in both groups. XRD indicated that both biomaterials were pure and crystalline. There was a statistically significant difference in granule area between the two groups after 15 days (P = 0.022; Student's t-test). After 15 days, an increased inflammatory response was seen in group 2 (P < 0.0001; ANOVA and Tukey's post hoc test) whereas it was more pronounced in group 1 after 30 days (P < 0.0001; ANOVA and Tukey's post hoc test). It was concluded that these biomaterials have similar physical, chemical and biocompatibility characteristics.
da Cruz
Ariadne Cristiane Cabral
AC
Department of Dentistry, State University of Ponta Grossa, Paraná, Brazil.
Pochapski
Márcia Thaís
MT
Daher
Josélia Borba
JB
da Silva
José Caetano Zurita
JC
Pilatti
Gibson Luiz
GL
Santos
Fábio André
FA
D000704:Analysis of Variance; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000633:toxicity*; D018786:Bone Substitutes / Q000737:chemistry / Q000633:toxicity*; D018360:Crystallography, X-Ray; D004577:Electron Probe Microanalysis; D006882:Hydroxyapatites / Q000737:chemistry / Q000633:toxicity*; D020007:Implants, Experimental; D008297:Male; D008855:Microscopy, Electron, Scanning; D010316:Particle Size; D051381:Rats; D017208:Rats, Wistar; D018709:Statistics, Nonparametric; D040521:Subcutaneous Tissue / Q000187:drug effects
D001672:Biocompatible Materials; D018786:Bone Substitutes; D006882:Hydroxyapatites