The permeability to water and cryoprotectants of immature and mature oocytes in the zebrafish (Danio rerio).
To identify a stage feasible for the cryopreservation of zebrafish oocytes, we investigated the permeability to water and cryoprotectants of immature (stage III) and mature (stage V) oocytes. The permeability to water (microm/min/atm) of immature oocytes at 25 degrees C (0.37) was significantly higher than that of mature oocytes (0.10). The permeability (x10(-3)cm/min) of immature oocytes to ethylene glycol, propylene glycol, and Me(2)SO (1.49-3.03) at 25 degrees C was substantially higher than that of mature oocytes approximately 0. The permeability of immature oocytes to glycerol was also high (1.75), although the permeability could not be measured in mature oocytes. Immature oocytes would be more suitable than mature oocytes for conservation of the zebrafish.
Seki
Shinsuke
S
Laboratory of Animal Science, College of Agriculture, Kochi University, Nankoku, Kochi 783-8502, Japan.
Kouya
Toshimitsu
T
Valdez
Delgado M
DM
Jin
Bo
B
Hara
Takao
T
Saida
Naoya
N
Kasai
Magosaburo
M
Edashige
Keisuke
K
D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000378:metabolism*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D019855:Ethylene Glycol / Q000494:pharmacology; D009865:Oocytes / Q000378:metabolism*; D019946:Propylene Glycol / Q000494:pharmacology; D013997:Time Factors; D014867:Water / Q000378:metabolism*; D015027:Zebrafish / Q000378:metabolism*
D003451:Cryoprotective Agents; D014867:Water; D019946:Propylene Glycol; D019855:Ethylene Glycol; D004121:Dimethyl Sulfoxide