[In vitro study on the differentiation of bone marrow mesenchymal stem cells into epidermal stem cells].
OBJECTIVE
To explore the feasibility of the differentiation of bone marrow mesenchymal stem cells into epidermal stem cells.


METHODS
Human bone mesenchymal stem cells (BMSCs) were isolated from the bone marrow of volunteers' ilium, and were purified and cultured in vitro and induced by supernatant liquid of HaCat cells. The morphologic changes of BMSCs were observed with electron microscope, and immunohistochemical examination was carried out to detect the positive staining of cytokeratin 10, cytokeratin 19 and integrin beta1 after one week induction.


RESULTS
Large numbers of flat-polygon cells were closely connected and developed like road metal. The plenty threadlike keratins were observed in the cytoplasm. The immunohistochemical examination showed the positive staining of few cytokeratin 10, a great deal of cytokeratin 19 and integrin beta1 after the one week induction.


CONCLUSIONS
Human BMSCs can differentiate into epidermal stem cells with supernatant liquid of HaCat cells and EGFs in vitro.
Long
Jian-hong
JH
Department of Burns and Plastics, Xiangya Hospital, Central South University, Changsha 410008, China. jianhong.long@gmail.com
Liu
Fang-fen
FF
Qi
Min
M
D000328:Adult; D001854:Bone Marrow Cells / Q000166:cytology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology*; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology*; D055815:Young Adult