title: Celiac disease serology in dermatitis herpetiformis. Which is the best option for detecting gluten sensitivity?
abstract: BACKGROUND
  Dermatitis herpetiformis (DH), a well-established gluten-sensitive skin disorder presenting variable degrees of enteropathy, constitutes a very useful model in order to assess the utility of the celiac disease (CD)-related serology in patients with mild intestinal damage.
  
  
  OBJECTIVE
  Our aim was to explore comparatively the performance of a panel of CD-related serologic tests in patients with DH.
  
  
  METHODS
  We assessed a series of 18 consecutive patients with skin biopsy proven DH presenting the overall spectrum of intestinal damage ranging from normal mucosa (n = 6) to total villous atrophy (TVA) (n = 6) through partial villous atrophy (PVA) (n = 6). Sera were obtained from all patients while consuming a gluten containing diet. Serologic tests were antiendomysial, anti-tissue transglutaminase and antigliadin antibodies, and newly developed tests detecting both antibody isotypes (IgA and IgG) against deamidated synthetic gliadin-derived peptides (a-GDP).
  
  
  RESULTS
  Serologic tests had a variable behaviour depending on the degree of enteropathy. While the majority of tests detected patients with TVA, only 50% of those with normal histology had positive assays. Patients with PVA had discordant results. Classical CD-specific tests were positive in only some patients with mild damage while all of them were identifed by a single assay detecting both isotypes of a-GDR CONCLUSION: The detection of a-GDP antibodies was the most reliable tool in order to identify gluten sensitivity in DH patients presenting a wide range of intestinal damage. Further studies should explore if these findings can be extrapolated to patients with CD having mild enteropathy.
authors.affiliation: Small Bowel Section, Department of Medicine, Hospital de Gastroenterología "Dr. Carlos Bonorino Udaondo", Buenos Aires, Argentina.
authors.forename: Emilia
authors.initials: E
authors.lastname: Sugai
authors.forename: Edgardo
authors.initials: E
authors.lastname: Smecuol
authors.forename: Sonia
authors.initials: S
authors.lastname: Niveloni
authors.forename: Horacio
authors.initials: H
authors.lastname: Vázquez
authors.forename: Marcelo
authors.initials: M
authors.lastname: Label
authors.forename: Roberto
authors.initials: R
authors.lastname: Mazure
authors.forename: Andrea
authors.initials: A
authors.lastname: Czech
authors.forename: Zulema
authors.initials: Z
authors.lastname: Kogan
authors.forename: Eduardo
authors.initials: E
authors.lastname: Mauriño
authors.forename: Julio C
authors.initials: JC
authors.lastname: Bai
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001284:Atrophy; D001323:Autoantibodies / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D001706:Biopsy; D002446:Celiac Disease / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D003874:Dermatitis Herpetiformis / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D005260:Female; D005903:Gliadin / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D011446:Prospective Studies; D012720:Severity of Illness Index; D011503:Transglutaminases / Q000276:immunology
chemical_list: D001323:Autoantibodies; D015415:Biomarkers; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D005903:Gliadin; D011503:Transglutaminases