title: Cysteinyl leukotriene upregulates IL-11 expression in allergic airway disease of mice.
abstract: BACKGROUND
  Chronic airway inflammation and airway remodeling are important features of bronchial asthma. IL-11 is one of the important mediators involved in the process of airway inflammation and remodeling. Cysteinyl leukotrienes (cysLTs) play roles in recruitment of inflammatory cells, airway smooth muscle contraction, vascular leakage, increased mucus secretion, decreased mucociliary clearance, and airway fibrosis.
  
  
  OBJECTIVE
  An aim of the present study was to determine the effect of the cysLTs on the regulation of IL-11 expression.
  
  
  METHODS
  We used a C57BL/6 mouse model of allergic airway disease and murine tracheal epithelial cells to examine the effects of cysLTs on the regulation of IL-11 expression.
  
  
  RESULTS
  Our present study with an ovalbumin-induced murine model of allergic airway disease revealed that levels of leukotriene C(4) (LTC(4)) in bronchoalveolar lavage fluids were increased and that administration of montelukast or pranlukast reduced the increased levels of LTC(4); the increased expression of IL-11 protein and mRNA in lung tissues; airway inflammation, bronchial hyperresponsiveness; the increased levels of TGF-beta(1), IL-4, and IL-13 in bronchoalveolar lavage fluids and lung tissues; and airway fibrosis. In addition, LTC(4) stimulates epithelial cells to produce IL-11. Our results also showed that cysLT type 1 receptor antagonists downregulated the activity of a transcription factor, nuclear factor kappaB, and BAY 11-7085 substantially reduced the increased levels of IL-11 after ovalbumin inhalation.
  
  
  CONCLUSIONS
  These results suggest that cysLTs regulate the IL-11 expression in allergic airway disease.
  
  
  CONCLUSIONS
  These findings provide one of the molecular mechanisms for the effects of cysLTs on airway inflammation and fibrosis in allergic airway diseases.
authors.affiliation: Department of Internal Medicine, Airway Remodeling Laboratory, Chonbuk National University Medical School, Jeonju, South Korea.
authors.forename: Kyung Sun
authors.initials: KS
authors.lastname: Lee
authors.forename: So Ri
authors.initials: SR
authors.lastname: Kim
authors.forename: Hee Sun
authors.initials: HS
authors.lastname: Park
authors.forename: Seoung Ju
authors.initials: SJ
authors.lastname: Park
authors.forename: Kyung Hoon
authors.initials: KH
authors.lastname: Min
authors.forename: Ka Young
authors.initials: KY
authors.lastname: Lee
authors.forename: Sun Mi
authors.initials: SM
authors.lastname: Jin
authors.forename: Yong Chul
authors.initials: YC
authors.lastname: Lee
mesh_terms: D000085:Acetates / Q000494:pharmacology; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002478:Cells, Cultured; D002867:Chromones / Q000494:pharmacology; D003094:Collagen / Q000378:metabolism; D003521:Cyclopropanes; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005260:Female; D017370:Interleukin-11 / Q000096:biosynthesis* / Q000235:genetics; D018793:Interleukin-13 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D020024:Leukotriene Antagonists / Q000494:pharmacology; D017997:Leukotriene C4 / Q000494:pharmacology*; D017998:Leukotriene D4 / Q000494:pharmacology*; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D009570:Nitriles / Q000494:pharmacology; D010047:Ovalbumin; D011804:Quinolines / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D012130:Respiratory Hypersensitivity / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D013440:Sulfides; D013450:Sulfones / Q000494:pharmacology; D051996:Transcription Factor RelA / Q000096:biosynthesis
chemical_list: D000085:Acetates; C416282:BAY 11-7085; D002867:Chromones; D003521:Cyclopropanes; D017370:Interleukin-11; D018793:Interleukin-13; D020024:Leukotriene Antagonists; D009570:Nitriles; D011804:Quinolines; D012333:RNA, Messenger; D013440:Sulfides; D013450:Sulfones; D051996:Transcription Factor RelA; D015847:Interleukin-4; D017997:Leukotriene C4; D017998:Leukotriene D4; D010047:Ovalbumin; D003094:Collagen; C093875:montelukast; C047681:pranlukast