Prediction of effective genome size in metagenomic samples.
We introduce a novel computational approach to predict effective genome size (EGS; a measure that includes multiple plasmid copies, inserted sequences, and associated phages and viruses) from short sequencing reads of environmental genomics (or metagenomics) projects. We observe considerable EGS differences between environments and link this with ecologic complexity as well as species composition (for instance, the presence of eukaryotes). For example, we estimate EGS in a complex, organism-dense farm soil sample at about 6.3 megabases (Mb) whereas that of the bacteria therein is only 4.7 Mb; for bacteria in a nutrient-poor, organism-sparse ocean surface water sample, EGS is as low as 1.6 Mb. The method also permits evaluation of completion status and assembly bias in single-genome sequencing projects.
Raes
Jeroen
J
European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany. raes@embl.de
Korbel
Jan O
JO
Lercher
Martin J
MJ
von Mering
Christian
C
Bork
Peer
P
D016477:Artifacts; D004777:Environment; D005819:Genetic Markers; D016680:Genome, Bacterial / Q000235:genetics*; D023281:Genomics / Q000379:methods*; D008957:Models, Genetic; D011387:Prokaryotic Cells / Q000378:metabolism; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA
D005819:Genetic Markers