title: Intimin types determined by heteroduplex mobility assay of intimin gene (eae)-positive Escherichia coli strains.
abstract: We developed a quick genetic approach to screen variants of the intimin gene (eae) by using a heteroduplex mobility assay (HMA) that targets the 5' conserved region of eae. The eae variants were categorized into 4 major HMA types and 10 minor subtypes.
authors.affiliation: Infectious Diseases Surveillance Center, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama-shi, Tokyo 208-0011, Japan. kito@nih.go.jp
authors.forename: Kenitiro
authors.initials: K
authors.lastname: Ito
authors.forename: Mariko
authors.initials: M
authors.lastname: Iida
authors.forename: Mitsugu
authors.initials: M
authors.lastname: Yamazaki
authors.forename: Kazuo
authors.initials: K
authors.lastname: Moriya
authors.forename: Sanae
authors.initials: S
authors.lastname: Moroishi
authors.forename: Jun
authors.initials: J
authors.lastname: Yatsuyanagi
authors.forename: Takayuki
authors.initials: T
authors.lastname: Kurazono
authors.forename: Noriaki
authors.initials: N
authors.lastname: Hiruta
authors.forename: Orn-Anong
authors.initials: OA
authors.lastname: Ratchtrachenchai
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics* / Q000378:metabolism; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D014644:Genetic Variation; D020180:Heteroduplex Analysis / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012703:Serotyping
chemical_list: D018829:Adhesins, Bacterial; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C073819:eaeA protein, E coli