The growth advantage in stationary-phase (GASP) phenomenon in mixed cultures of enterobacteria.
Growth advantage in stationary phase (GASP) is the term used to describe the ability of mutants with an increased fitness from 10-day-old enterobacteria culture to out-compete 1-day-old cells of the same initial strain during a prolonged stationary phase, although the aged cells are introduced as a minority. We studied this bacterial trait in mixed cultures of two enterobacterial species, Escherichia coli and Salmonella enterica, wild type in addition to derived mutants from both strains that contain chromosomal-encoded resistance to either nalidixic acid or streptomycin. The strong GASP phenotype was obtained in mixed cultures with the aged mutant strains, but not when the isogenic antibiotic-sensitive strains were used. This phenomenon was associated with chromosomal rearrangements in 10-day-old bacterial antibiotic-resistant mutated cells.
Bacun-Druzina
Visnja
V
Laboratory of Biology and Microbial Genetics, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia. visnjabd@pbf.hr
Cagalj
Zeljka
Z
Gjuracic
Kresimir
K
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001431:Bacteriological Techniques; D002876:Chromosomes, Bacterial; D018920:Coculture Techniques; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D009154:Mutation; D009268:Nalidixic Acid / Q000494:pharmacology; D010641:Phenotype; D012150:Polymorphism, Restriction Fragment Length; D019779:Salmonella enterica / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D013307:Streptomycin / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D009268:Nalidixic Acid; D013307:Streptomycin