The role of vitellogenin during gestation of Girardinichthys viviparus and Ameca splendens; two goodeid fish with matrotrophic viviparity.
Goodeid fish have matrotrophic viviparity, and unlike lecitotrophic fish, yolk loss forces the female to provide the nutritional requirements for embryonic development. Vitellogenin (VTG) is the yolk precursor protein synthesized in the maternal liver, but there is only circumstantial evidence regarding VTG supply during the ontogenesis of bony fish with matrotrophic viviparity. Therefore, the goal of the present study was to identify and quantify VTG during gestation of the black fin goodeid Girardinichthys viviparus and the butterfly split-fin goodeid Ameca splendens. Females at different gonadic developmental stages were selected in order to evaluate VTG mRNA expression in the maternal liver using RT-PCR; VTG quantification in maternal muscle and liver, as well as in the embryos, was done using ELISA, and immunohistochemical detection of VTG was done in the black fin goodeid. The results suggest that VTG supplies nutrients during embryonic development of both species, which have different life histories. It is possible that the transition from lecitotrophy to matrotrophic viviparity in bony fish with intraluminal gestation involved adaptive transition strategies that included changes in the relationship between oocytes and follicular cells, as well as a gradual loss of VTG synthesis during embryonic development.
Vega-López
Armando
A
Laboratorio de Toxicología Acuática, Departamento de Farmacia, Escuela Nacional de Ciencias Biológicas, IPN. Carpio y Plan de Ayala s/n, Col. Plutarco Elías Calles "Casco de Santo Tomás". D.F. CP 11340, México. Electronic address: avegadv@yahoo.com.mx.
Ortiz-Ordóñez
Esperanza
E
Laboratorio de Histología, Departamento de Morfología, Escuela Nacional de Ciencias Biológicas, IPN. Carpio y Plan de Ayala s/n, Col. Plutarco Elías Calles "Casco de Santo Tomás". D.F. CP 11340, México.
Uría-Galicia
Esther
E
Laboratorio de Histología, Departamento de Morfología, Escuela Nacional de Ciencias Biológicas, IPN. Carpio y Plan de Ayala s/n, Col. Plutarco Elías Calles "Casco de Santo Tomás". D.F. CP 11340, México.
Mendoza-Santana
E Laura
EL
Laboratorio de Histología, Departamento de Morfología, Escuela Nacional de Ciencias Biológicas, IPN. Carpio y Plan de Ayala s/n, Col. Plutarco Elías Calles "Casco de Santo Tomás". D.F. CP 11340, México.
Hernández-Cornejo
Rubí
R
Laboratorio de Biología Molecular, CIAD Mazatlán, Av. Sábalo Cerritos s/n, Mazatlán Sinaloa. CP 82010, México.
Atondo-Mexia
Roxana
R
Laboratorio de Biología Molecular, CIAD Mazatlán, Av. Sábalo Cerritos s/n, Mazatlán Sinaloa. CP 82010, México.
García-Gasca
Alejandra
A
Laboratorio de Biología Molecular, CIAD Mazatlán, Av. Sábalo Cerritos s/n, Mazatlán Sinaloa. CP 82010, México.
García-Latorre
Ethel
E
Laboratorio de Inmunoquímica I, Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, IPN. Carpio y Plan de Ayala s/n, Col. Plutarco Elías Calles "Casco de Santo Tomás". D.F. CP 11340, México.
Domínguez-López
Maria Lilia
ML
Laboratorio de Inmunoquímica I, Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, IPN. Carpio y Plan de Ayala s/n, Col. Plutarco Elías Calles "Casco de Santo Tomás". D.F. CP 11340, México.
D000818:Animals; D003532:Cyprinodontiformes / Q000196:embryology* / Q000235:genetics / Q000378:metabolism*; D047108:Embryonic Development; D005260:Female; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D008099:Liver / Q000378:metabolism; D008297:Male; D009865:Oocytes / Q000166:cytology; D006080:Ovarian Follicle / Q000166:cytology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014819:Vitellogenins / Q000235:genetics / Q000378:metabolism*; D052286:Viviparity, Nonmammalian
D014819:Vitellogenins