title: Characterization of the proteins of bacterial strain isolated from contaminated site involved in heavy metal resistance--a proteomic approach.
abstract: The present study describes response of a bacterial strain isolated from a polluted river to heavy metal toxicity. The bacterium was identified to be Klebsiella pneumoniae by biochemical tests using API 20E strips and 16S ribotyping. The isolate was studied for its tolerance to two heavy metals, i.e., cobalt (Co(2+)) and lead (Pb(2+)) by growing it in citrate mineral medium (CMM). Proteomic approach involving two-dimensional polyacrylamide gel electrophoresis (2D PAGE) and mass spectrometry (MS) was used to identify the differentially expressed proteins under heavy metal stress. Two of the differentially expressed proteins were identified to be l-isoaspartate protein carboxymethyltransferase type II and DNA gyrase A. To our knowledge, this is for the first time that K. pneumoniae has been reported to be present in metal contaminated site and l-isoaspartate protein carboxymethyltransferase type II protein to be over expressed under heavy metal stress. The role of these proteins in metal tolerance is discussed.
authors.affiliation: Department of Biotechnology, University of Pune, Pune 411007, India.
authors.forename: Chinmayee
authors.initials: C
authors.lastname: Bar
authors.forename: Rajendra
authors.initials: R
authors.lastname: Patil
authors.forename: Jignesh
authors.initials: J
authors.lastname: Doshi
authors.forename: Mahesh J
authors.initials: MJ
authors.lastname: Kulkarni
authors.forename: W N
authors.initials: WN
authors.lastname: Gade
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D001483:Base Sequence; D003035:Cobalt / Q000378:metabolism; D024881:Drug Resistance, Bacterial; D007711:Klebsiella pneumoniae / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D007854:Lead / Q000378:metabolism; D019216:Metals, Heavy / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D040901:Proteomics / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000737:chemistry; D045483:Rivers / Q000382:microbiology*; D014874:Water Pollutants, Chemical / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D019216:Metals, Heavy; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; D007854:Lead; D003035:Cobalt