title: Influence of growth medium on cometabolic degradation of polycyclic aromatic hydrocarbons by Sphingomonas sp. strain PheB4.
abstract: The influence of growth medium on cometabolic degradation of polycyclic aromatic hydrocarbons (PAHs) was investigated when Sphingomonas sp. strain PheB4 isolated from surface mangrove sediments was grown in either phenanthrene-containing mineral salts medium (PMSM) or nutrient broth (NB). The NB-grown culture exhibited a more rapid cometabolic degradation of single and mixed non-growth substrate PAHs compared to the PMSM-grown culture. The concentrations of PAH metabolites were also lower in NB-grown culture than in PMSM-grown culture, suggesting that NB-grown culture removed metabolites at a faster rate, particularly, for metabolites produced from cometabolic degradation of a binary mixture of PAHs. Cometabolic pathways of single PAH (anthracene, fluorene, or fluoranthene) in NB-grown culture showed similarity to that in PMSM-grown culture. However, cometabolic pathways of mixed PAHs were more diverse in NB-grown culture than that in PMSM-grown culture. These results indicated that nutrient rich medium was effective in enhancing cometabolic degradation of mixed PAHs concomitant with a rapid removal of metabolites, which could be useful for the bioremediation of mixed PAHs contaminated sites using Sphingomonas sp. strain PheB4.
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, PR China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Zhong
authors.forename: Tiangang
authors.initials: T
authors.lastname: Luan
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Wang
authors.forename: Chongyu
authors.initials: C
authors.lastname: Lan
authors.forename: Nora F Y
authors.initials: NF
authors.lastname: Tam
mesh_terms: D032407:Avicennia / Q000254:growth & development*; D001673:Biodegradation, Environmental; D002681:China; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology*; D019015:Geologic Sediments / Q000382:microbiology*; D010616:Phenanthrenes / Q000378:metabolism / Q000494:pharmacology; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry / Q000378:metabolism*; D020578:Sphingomonas / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification
chemical_list: D003470:Culture Media; D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons; C031181:phenanthrene