A single residue change in Vibrio harveyi hemolysin results in the loss of phospholipase and hemolytic activities and pathogenicity for turbot (Scophthalmus maximus).
Vibrio harveyi hemolysin, an important virulence determinant in fish pathogenesis, was further characterized, and the enzyme was identified as a phospholipase B by gas chromatography. Site-directed mutagenesis revealed that a specific residue, Ser153, was critical for its enzymatic activity and for its virulence in fish.
Sun
Boguang
B
Department of Marine Biology, Ocean University of China, 5 Yushan Road, Qingdao 266003, People's Republic of China.
Zhang
Xiao-Hua
XH
Tang
Xuexi
X
Wang
Shushan
S
Zhong
Yingbin
Y
Chen
Jixiang
J
Austin
Brian
B
D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005393:Fish Diseases / Q000382:microbiology*; D005412:Flatfishes / Q000382:microbiology*; D006460:Hemolysin Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006461:Hemolysis; D008958:Models, Molecular; D009154:Mutation; D010740:Phospholipases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014733:Vibrio / Q000201:enzymology / Q000235:genetics / Q000472:pathogenicity*; D014735:Vibrio Infections / Q000382:microbiology / Q000662:veterinary; D014774:Virulence
D001426:Bacterial Proteins; D006460:Hemolysin Proteins; C073845:hemolysin, Vibrio; D010740:Phospholipases