Biodegradation of crystal violet by Pseudomonas putida.
Crystal violet (CV), which has been extensively used as a biological stain and a commercial textile dye, is a recalcitrant molecule. A strain of Pseudomonas putida was isolated that effectively degraded CV: up to 80% of 60 microM CV as the sole carbon source, was degraded in liquid media within 1 week. Nine degradation products were isolated and identified. We propose that CV degradation occurs via a stepwise demethylation process to yield mono-, di-, tri-, tetra-, penta- and hexa-demethylated CV species.
Chen
Chiing-Chang
CC
Department of General Education, National Taichung Nursing College, Taichung, Taiwan.
Liao
Hung-Ju
HJ
Cheng
Chiu-Yu
CY
Yen
Chia-Yuen
CY
Chung
Ying-Chien
YC
D001673:Biodegradation, Environmental; D005840:Gentian Violet / Q000378:metabolism*; D016958:Pseudomonas putida / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D018508:Water Purification / Q000379:methods*
D014874:Water Pollutants, Chemical; D005840:Gentian Violet