title: Monitoring of hepatitis C virus quasispecies in chronic infection by matrix-assisted laser desorption ionization-time of flight mass spectrometry mutation detection.
abstract: Using both a mass spectrometry-based method and the classical method of cloning and sequencing, we demonstrated weekly changes in the hypervariable region 1 quasispecies of a chimpanzee infected with an infectious clone, coinciding with neutralizing antibody emergence. We also used the mass spectrometry method in the clinical follow-up of a chronically infected patient over a 5-year period.
authors.affiliation: Metabolism Research Program, The Hospital for Sick Children, Toronto, Canada.
authors.forename: Carmen
authors.initials: C
authors.lastname: Yea
authors.forename: Jens
authors.initials: J
authors.lastname: Bukh
authors.forename: Melissa
authors.initials: M
authors.lastname: Ayers
authors.forename: Eve
authors.initials: E
authors.lastname: Roberts
authors.forename: Mel
authors.initials: M
authors.lastname: Krajden
authors.forename: Raymond
authors.initials: R
authors.lastname: Tellier
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018420:Ape Diseases / Q000821:virology; D016174:Hepacivirus / Q000145:classification* / Q000235:genetics*; D019698:Hepatitis C, Chronic / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D002679:Pan troglodytes; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D014759:Viral Envelope Proteins; C091015:glycoprotein E2, Hepatitis C virus