title: [A new method for 5, 10-methylenetetrahydrofolate reductase single nucleotide polymorphisms genotyping used to study susceptibility of hematological malignancy].
abstract: The aim of this study was to set up a new method for 5, 10-Methylenetetrahydrofolate reductase (MTHFR) single nucleotide polymorphisms (SNP) genotyping, and to investigate the hereditary susceptibility of hematological malignancy. Prepared an aimed gene microarray based on cDNA microarray theory, dual-color fluorescence hybridization was used to detect SNP loci, and DNA sequencing was performed to confirm the results. The MTHFR C677T SNP loci of 157 controls and 127 patients with hematological malignancies (30 multiple myeloma, 28 non-Hodgkin's lymphoma, 22 acute lymphoblastic leukemia, 40 acute myeloid leukemia, 7 chronic myeloid leukemia) from Jiangsu province were detected. The results showed that after overlapping, homozygous wild type, heterozygote type and homozygous mutant type yielded green, yellow and red fluorescence, respectively. DNA sequencing validated these results. The allele frequency of 677C and 677T in patients and controls were 58.7% and 66.9%, 41.3% and 33.1% respectively, showing statistically significant difference (chi2 = 4.077, P = 0.043). 677TT genotype showed a significantly higher risk of MM (OR = 4.21; 95% CI = 1.50 - 11.83; P = 0.006). It is concluded that this microarray-based method is accurate, high-throughput and inexpensive, suitable for SNP genotyping in a large number of individuals. C677T polymorphisms influence the risk of hematological malignancies. 677TT genotype is susceptive to MM.
authors.affiliation: Department of Hematology, Zhongda Hospital, Sontheast University, Nanjing 210009, China. bachen@seu.edu.cn
authors.forename: Bao-An
authors.initials: BA
authors.lastname: Chen
authors.forename: Ni
authors.initials: N
authors.lastname: Jiang
authors.forename: Mei-Ju
authors.initials: MJ
authors.lastname: Ji
authors.forename: Peng
authors.initials: P
authors.lastname: Hou
authors.forename: Zu-Hong
authors.initials: ZH
authors.lastname: Lu
authors.forename: Chong
authors.initials: C
authors.lastname: Gao
authors.forename: Jia-Hua
authors.initials: JH
authors.lastname: Ding
authors.forename: Yun-Yu
authors.initials: YY
authors.lastname: Sun
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Cheng
authors.forename: Gang
authors.initials: G
authors.lastname: Zhao
mesh_terms: D000328:Adult; D000368:Aged; D001483:Base Sequence; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D019337:Hematologic Neoplasms / Q000201:enzymology / Q000235:genetics*; D006801:Humans; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008875:Middle Aged; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide
chemical_list: D042965:Methylenetetrahydrofolate Reductase (NADPH2)