title: A type IV-secretion-like system is required for conjugative DNA transport of broad-host-range plasmid pIP501 in gram-positive bacteria.
abstract: Plasmid pIP501 has a very broad host range for conjugative transfer among a wide variety of gram-positive bacteria and gram-negative Escherichia coli. Functionality of the pIP501 transfer (tra) genes in E. coli was proven by pIP501 retrotransfer to Enterococcus faecalis (B. Kurenbach, C. Bohn, J. Prabhu, M. Abudukerim, U. Szewzyk, and E. Grohmann, Plasmid 50:86-93, 2003). The 15 pIP501 tra genes are organized in a single operon (B. Kurenbach, J. Kopeć, M. Mägdefrau, K. Andreas, W. Keller, C. Bohn, M. Y. Abajy, and E. Grohmann, Microbiology 152:637-645, 2006). The pIP501 tra operon is negatively autoregulated at the transcriptional level by the conjugative DNA relaxase TraA. Three of the 15 pIP501-encoded Tra proteins show significant sequence similarity to the Agrobacterium type IV secretion system proteins VirB1, VirB4, and VirD4. Here we report a comprehensive protein-protein interaction map of all of the pIP501-encoded Tra proteins determined by the yeast two-hybrid assay. Most of the interactions were verified in vitro by isolation of the protein complexes with pull-down assays. In conjunction with known or postulated functions of the pIP501-encoded Tra proteins and computer-assisted prediction of their cellular location, we propose a model for the first type IV-secretion-like system encoded by a conjugative plasmid from gram-positive bacteria.
authors.affiliation: Department of Environmental Microbiology/Genetics, FR1-2, Franklinstrasse 28/29, University of Technology Berlin, D-10587 Berlin, Germany.
authors.forename: Mohammad Y
authors.initials: MY
authors.lastname: Abajy
authors.forename: Jolanta
authors.initials: J
authors.lastname: Kopeć
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Schiwon
authors.forename: Michal
authors.initials: M
authors.lastname: Burzynski
authors.forename: Mike
authors.initials: M
authors.lastname: Döring
authors.forename: Christine
authors.initials: C
authors.lastname: Bohn
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Grohmann
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D003227:Conjugation, Genetic; D004254:DNA Nucleotidyltransferases / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D013293:Enterococcus faecalis / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D022761:Gene Transfer, Horizontal; D016366:Open Reading Frames / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D020798:Two-Hybrid System Techniques
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004254:DNA Nucleotidyltransferases; C110340:DNA relaxase