Karyometric analysis of nasal polyps.
OBJECTIVE
Nuclear characteristics of epithelial cells in nasal polyps were analyzed.


METHODS
The slides of 35 patients who underwent surgery for nasal polyposis and 18 slides of normal mucosa were examined. The specimens were stained with hematoxylin-eosin. Tissue sections were analyzed by a CCD camera and the Karyotype Program of Human Cytogenetics Nomenclature System on a Macintosh computer (OS 9). Fifty epithelial cells in each slide were randomly selected and transferred to the automated karyotyping system and photographed. The diameters of the nuclei were measured and some peculiar nuclear features were examined, including unsmooth appearance of the nuclear membrane, anaphase-bridge, and binucleation.


RESULTS
There were significant differences between nasal polyp and normal mucosa groups in terms of nuclear diameter, perimeter, and volume, with all variables being greater in the nasal polyp group (p=0.001). Unusual nuclear features differed significantly (unsmooth nuclear membrane appearance, p<0.001; anaphase-bridge, p=0.041; binucleation, p=0.018), as well.


CONCLUSIONS
Epithelial cells of nasal polyps exhibit nuclear instability.
Yildirim
Altan
A
Department of Otolaryngology, Medicine Faculty of Cumhuriyet University, Sivas, Turkey. altan11@hotmail.com
Ozdemir
Oztürk
O
Gül
Eylem
E
Yildirim
Beyazit
B
Elagöz
Sahende
S
Kunt
Tanfer
T
D000328:Adult; D016022:Case-Control Studies; D002467:Cell Nucleus / Q000473:pathology*; D004847:Epithelial Cells / Q000473:pathology*; D005260:Female; D006801:Humans; D007620:Karyometry; D007621:Karyotyping; D008297:Male; D009297:Nasal Mucosa / Q000166:cytology; D009298:Nasal Polyps / Q000473:pathology*