Cytological analysis of the mother cell death process during sporulation in Bacillus subtilis.
We have identified the following events during the late stage in the mother cell in Bacillus subtilis: spore detachment from the polar site of the mother cell, membrane rupture, cell wall collapse, and release of the free spore. The membrane rupture was followed by mother cell lysis. Moreover, we found that NucB, an extracellular nuclease, is involved in DNA degradation after mother cell lysis.
Hosoya
Shigeo
S
International Environmental and Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
Lu
Zuolei
Z
Ozaki
Yousuke
Y
Takeuchi
Michio
M
Sato
Tsutomu
T
D001412:Bacillus subtilis / Q000166:cytology* / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001433:Bacteriolysis; D003470:Culture Media; D004269:DNA, Bacterial / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods*; D009154:Mutation; D013171:Spores, Bacterial / Q000502:physiology*; D013997:Time Factors
D001426:Bacterial Proteins; D003470:Culture Media; D004269:DNA, Bacterial; D003851:Deoxyribonucleases