[Effect of three-step sequential method on Th1/Th2 in patients with corticosteroid-dependent asthma].
OBJECTIVE
To investigate the therapeutic mechanism of three-step sequential method (TSSM) on patients with corticosteroid-dependent asthma (SDA).


METHODS
Forty patients with SDA were randomly assigned according to the randomizing number table to two groups equally, the treated group treated with three-step sequential recipes plus inhalation of Pulmicort Turbuhaler 200 microg, twice a day, and the control group treated with Pulmicort Turbuhaler alone. The therapeutic course for both groups was 12 - 14 weeks. Changes of the symptom score of asthma, the corticosteroid dosage used and the lung function were observed and the positive expression rate of IFN-gamma and IL-4 in peripheral CD4+ T cells were determined by flow cytometry before and after treatment.


RESULTS
There was significant difference in the asthma symptom score, the oral corticosteroid dosage and the lung function between the treated group and the control group after treatment (P < 0.01). The expression rate of Th2 reduced, the ratio of Th1/Th2 increased significantly after treatment in both groups (P < 0.01, P < 0.05), but the changes were more remarkable in the treated group than those in the control group, showing significant difference between them (P < 0.01), while the expression rate of Th1 had no obvious change after treatment with no significant difference shown between the two groups (P > 0.05).


CONCLUSIONS
TSSM can regulate imbalance of Th1/Th2, inhibit generation of inflammatory cytokines, decrease airway hyper-response, and therefore improve the pulmonary function, alleviate the asthmatic symptoms and reduce the patients' dependence on corticosteroid.
Cui
Hong-Sheng
HS
Department of Respiratory Diseases, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing. hshcui@hotmail.com
Cui
Wei
W
Wen
Zhi-Hao
ZH
D000328:Adult; D001249:Asthma / Q000188:drug therapy*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000627:therapeutic use*; D005260:Female; D005938:Glucocorticoids / Q000009:adverse effects / Q000627:therapeutic use*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008517:Phytotherapy / Q000379:methods*; D019966:Substance-Related Disorders / Q000188:drug therapy / Q000209:etiology; D016176:T-Lymphocyte Subsets / Q000187:drug effects; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects*
D004365:Drugs, Chinese Herbal; D005938:Glucocorticoids