title: Molecular characterization of infectious bursal disease virus (IBDV): diversity of very virulent IBDV in Tanzania.
abstract: Nucleotide sequences of the VP2 hypervariable region (VP2-HVR) of 14 infectious bursal disease viruses (IBDVs) isolated in Tanzania from 2001 to 2004 were determined. Phylogenetic analysis showed that the isolates diverged into two genotypes and belonged to the very virulent (VV) type. In the phylogenetic tree, strains in one genotype clustered in a distinct group and were closely related to some strains isolated in western Africa, with nucleotide similarities of 96.1-96.8%, while strains in another genotype were clustered within the European/Asian VV type with nucleotide similarities ranging from 97.5 to 99.3%. Both genotypes were widely distributed throughout Tanzania, and had conserved putative virulence marker amino acids (aa) at positions 222(A), 242(I), 256(I), 294(I) and 299(S). Our findings demonstrate for the first time the existence of both African and European/Asian VV-IBDV variants in Tanzania.
authors.affiliation: Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan.
authors.forename: C J
authors.initials: CJ
authors.lastname: Kasanga
authors.forename: T
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: P N
authors.initials: PN
authors.lastname: Wambura
authors.forename: A D
authors.initials: AD
authors.lastname: Maeda-Machang'u
authors.forename: K
authors.initials: K
authors.lastname: Ohya
authors.forename: H
authors.initials: H
authors.lastname: Fukushi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018175:Birnaviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D002645:Chickens / Q000821:virology*; D017124:Conserved Sequence; D005838:Genotype; D007243:Infectious bursal disease virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D008969:Molecular Sequence Data; D010802:Phylogeny; D011201:Poultry Diseases / Q000453:epidemiology / Q000821:virology*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013636:Tanzania / Q000453:epidemiology; D015678:Viral Structural Proteins / Q000737:chemistry / Q000235:genetics*; D014774:Virulence / Q000235:genetics
chemical_list: C082513:VP2 protein, infectious bursal disease virus; D015678:Viral Structural Proteins