Generic detection of coronaviruses and differentiation at the prototype strain level by reverse transcription-PCR and nonfluorescent low-density microarray.
A nonfluorescent low-cost, low-density oligonucleotide array was designed for detecting the whole coronavirus genus after reverse transcription (RT)-PCR. The limit of detection was 15.7 copies/reaction. The clinical detection limit in patients with severe acute respiratory syndrome was 100 copies/sample. In 39 children suffering from coronavirus 229E, NL63, OC43, or HKU1, the sensitivity was equal to that of individual real-time RT-PCRs.
de Souza Luna
Luciano Kleber
LK
Clinical Virology Section, Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Str. 74, 20359 Hamburg, Germany.
Heiser
Volker
V
Regamey
Nicolas
N
Panning
Marcus
M
Drexler
Jan Felix
JF
Mulangu
Sabue
S
Poon
Leo
L
Baumgarte
Sigrid
S
Haijema
Bert Jan
BJ
Kaiser
Laurent
L
Drosten
Christian
C
D015373:Bacterial Typing Techniques; D017934:Coronavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D028941:Coronavirus 229E, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D028962:Coronavirus OC43, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D045473:Severe acute respiratory syndrome-related coronavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D013045:Species Specificity
D012367:RNA, Viral