title: Erratum to "Diapause-associated metabolic traits reiterated in long-lived daf-2 mutants in the nematode Caenorhabditis elegans" [Mech. Ageing Dev. 127 (5) (2006) 458-472].
abstract: The longevity of the Caenorhabditis elegans diapausal dauer larva greatly exceeds that of the adult. Dauer formation and adult ageing are both regulated by insulin/IGF-1 signalling (IIS). Reduced IIS, e.g. by mutation of the daf-2 insulin/IGF-1 receptor gene, increases adult lifespan. This may reflect mis-expression in the adult of dauer longevity-assurance processes. Since IIS plays a central role in the regulation of metabolism, metabolic alterations shared by dauer larvae and daf-2 adults represent candidate mechanisms for lifespan determination. We have conducted a detailed comparison of transcript profile data from dauers and daf-2 mutant adults, focusing on expression of metabolic pathway genes. Our results imply up-regulation in both dauers and daf-2 mutant adults of gluconeogenesis, glyoxylate pathway activity, and trehalose biosynthesis. Down-regulation of the citric acid cycle and mitochondrial respiratory chain occurs in dauers, but not daf-2 adults. However, the F1 ATPase inhibitor was up-regulated in both, implying enhanced homeostasis in conditions where mitochondria are stressed. Overall, the data implies increased conversion of fat to carbohydrate, and conservation of ATP stocks in daf-2 mutant adults, suggesting a state of increased energy availability. We postulate that this fuels increased somatic maintenance activity, as suggested by the disposable soma theory.
authors.affiliation: Department of Biology, University College London, Gower Street, London WC1E 6BT, UK.
authors.forename: Joshua J
authors.initials: JJ
authors.lastname: McElwee
authors.forename: Eugene
authors.initials: E
authors.lastname: Schuster
authors.forename: Eric
authors.initials: E
authors.lastname: Blanc
authors.forename: Janet
authors.initials: J
authors.lastname: Thornton
authors.forename: David
authors.initials: D
authors.lastname: Gems
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics*; D004734:Energy Metabolism / Q000235:genetics*; D007814:Larva / Q000235:genetics; D008136:Longevity / Q000235:genetics*; D009154:Mutation; D011972:Receptor, Insulin / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics*
chemical_list: D029742:Caenorhabditis elegans Proteins; C107483:DAF-2 protein, C elegans; D011972:Receptor, Insulin