Biocompatible porous ceramics for the cultivation of hematopoietic cells.
Porous ceramics made of alumina and hydroxyapatite were created using a protein foaming method. Porosity and pore size distribution were successfully varied by means of chemical modification of the foaming protein Bovine serum albumin (BSA). The effectiveness of the BSA and of its chemical modifications as well as the influence of the dispersing agent were investigated using synchrotron tomography. Resulting porous ceramic materials were used as three-dimensional substrates for the cultivation of human peripheral stem cells. The cells proliferated and differentiated in culture. Five cell lines consistent with human blood cell lines were observed.
Berthold
Almuth
A
TU Berlin, Institut für Werkstoffwissenschaft und -technologien, Englische Strasse 20, 10587 Berlin, Germany. almuth.berthold@ms.tu-berlin.de
Haibel
Astrid
A
Brandes
Natascha
N
Kroh
Lothar
L
Gross
Ulrich
U
Uharek
Lutz
L
Schubert
Helmut
H
D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D002516:Ceramics / Q000737:chemistry*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D008422:Materials Testing; D016062:Porosity; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials