Complexation of fatty acids and fatty acid-CoAs with synthetic O-methylated polysaccharides.
An experimentally simple, but highly reproducible and reliable method has been developed to follow the complexation event of fatty acid (FA) and FA-CoA with polysaccharides. This method was based on the recent discovery of the unique blue-shifted UV absorption associated with the aggregation of tetraenoic fatty acids (TE-FAs) in aqueous solution. Complexation was monitored by recording the intensity of UV absorption at 250 nm ([free TE-FA]) and 303 nm ([complexed TE-FA]), and the K aggregate of C(20) t,t,t,t-TE-FA exhibited the ideal property for this purpose. Synthetic 3-O-methyl-D-mannose- and 6-O-methyl-D-glucose-containing lipopolysaccharides were found to exhibit a broad range of the binding affinities with C(20) t,t,t,t-TE-FAs as well as saturated FAs/FA-CoAs.
Cheon
Hwan-Sung
HS
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA.
Wang
Yonghui
Y
Ma
Jianguo
J
Kishi
Yoshito
Y
D000214:Acyl Coenzyme A / Q000737:chemistry*; D005227:Fatty Acids / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D008766:Methylmannosides / Q000737:chemistry*; D015394:Molecular Structure; D011134:Polysaccharides / Q000737:chemistry*
D000214:Acyl Coenzyme A; D005227:Fatty Acids; D005456:Fluorescent Dyes; D008766:Methylmannosides; D011134:Polysaccharides; C043123:3-O-methylmannose