title: Degradation of 2-nitrobenzoate by Burkholderia terrae strain KU-15.
abstract: Bacterial strain KU-15, identified as a Burkholderia terrae by 16S rRNA gene sequence analysis, was one of 11 new isolates that grew on 2-nitrobenzoate as sole source of carbon and nitrogen. Strain KU-15 was also found to grow on anthranilate, 4-nitrobenzoate, and 4-aminobenzoate. Whole cells of strain KU-15 were found to accumulate ammonia in the medium, indicating that the degradation of 2-nitrobenzoate proceeds through a reductive route. Metabolite analyses by high-performance liquid chromatography indicated that 3-hydroxyanthranilate, anthranilate, and catechol are intermediates of 2-nitrobenzoate metabolism in strain KU-15. Enzyme studies suggested that 2-nitrobenzoate degradation occurs via the formation of 2-hydroxylaminobenzoate and that the pathway branches at this point to form two different aromatic intermediates: anthranilate and 3-hydroxyanthranilate. PCR amplifications and DNA sequencing revealed DNA fragments encoding a polypeptide homologous to 2-amino-3-carboxymuconate 6-semialdehyde decarboxylase and anthranilate 1,2-dioxygenase.
authors.affiliation: Department of Biotechnology, Faculty of Engineering, Kansai University, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Iwaki
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Hasegawa
mesh_terms: D019117:Burkholderia / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002262:Carboxy-Lyases / Q000235:genetics / Q000378:metabolism; D002457:Cell Extracts; D002851:Chromatography, High Pressure Liquid; D049308:Dioxygenases / Q000378:metabolism; D015394:Molecular Structure; D009579:Nitrobenzoates / Q000737:chemistry / Q000378:metabolism*; D010802:Phylogeny; D013057:Spectrum Analysis; D062367:ortho-Aminobenzoates / Q000378:metabolism
chemical_list: C518437:2-nitrobenzoate; D002457:Cell Extracts; D009579:Nitrobenzoates; D062367:ortho-Aminobenzoates; C031385:anthranilic acid; D049308:Dioxygenases; D002262:Carboxy-Lyases