6-Chloro-N,N-diethyl-1,3,5-triazine-2,4-diamine (CAT) sensor based on biomimetic recognition utilizing a molecularly imprinted artificial receptor.
We aimed to develop a 6-chloro-N,N-diethyl-1,3,5-triazine-2,4-diamine (CAT)-sensing system based on a biomimetic receptor of a molecularly imprinted polymer for CAT and electrochemical determination of CAT. A molecularly imprinted polymer for CAT was prepared by the polymerization of methacrylic acid (MAA) as a functional monomer and ethylene glycol dimethacrylate (EDMA) as a cross-linker with a template molecule (CAT) in dimethyl formamide (DMF). The polymer prepared with the ratio of these monomers (CAT:MAA:EDMA = 1:7.5:20) showed the most selective rebinding to CAT, and the obtained polymer was recognized as a CAT-imprinted polymer (CAT-MIP). The effect of the specific imprinting sites of CAT-MIP was demonstrated by Scatchard analysis. In an aqueous solution of CAT, CAT-MIP showed the maximum binding of CAT in a 0.05 M phosphate buffer (PB), pH 5.0. The binding amount of CAT to CAT-MIP was 24% more than atrazine and 72% more than propazine. The CAT-sensing system was composed of a column of CAT-MIP particles and a voltammetry analyzer. The reductive current of CAT depended on the concentration of CAT up to 30 microM with the system.
Fuchiwaki
Yusuke
Y
Department of Bioengineering, Faculty of Engineering, Soka University, Tokyo, Japan.
Shimizu
Akio
A
Kubo
Izumi
I
D040761:Biomimetic Materials; D004563:Electrochemistry; D006046:Gold; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D008628:Mercury; D010316:Particle Size; D011048:Polarography; D011955:Receptors, Drug / Q000737:chemistry*; D012997:Solvents; D014227:Triazines / Q000138:chemical synthesis / Q000737:chemistry*
C517869:6-chloro-N,N-dimethyl-1,3,5-triazine-2,4-diamine; D007202:Indicators and Reagents; D011955:Receptors, Drug; D012997:Solvents; D014227:Triazines; D006046:Gold; D008628:Mercury