title: Morphological and biochemical alterations in the jejunum following iodoacetamide-induced colitis in rats.
abstract: This study aims to describe the morphological alterations in the small and large intestines as well as the expression of some enterocyte enzymes and carriers in a rat model of iodoacetamide-induced colitis. Biopsies from the large and small intestines were taken at 1, 2, 4, 8, and 16 days postinduction and studied by light microscopy. The expressions of lactase, sucrase, aminopeptidase, and Glut-5 in the jejunum were studied by immunohistochemistry. Gene expressions of enterocyte lactase and sucrase were determined by RT-PCR using specific oligonucleotides. Microscopic examination of the large intestines revealed manifestations concordant with inflammation. Such alterations peaked at 2 days, were maintained to a lesser extent for 4 days, regressed by 8 days, and healed by 16 days. In the jejunum, the expression of lactase, sucrase, and aminopeptidase decreased 2 days after colitis induction, and recovered 2 days later. Similarly, Glut-5 expression decreased transiently with partial recovery by day 8. Compared with sham, gene expression of jejunal brush border enzymes sucrase and lactase showed a 4-fold increase in lactase and a 9-fold increase in sucrase after 4 days. We conclude that colitis can induce significant functional abnormalities in distant noninflamed small bowel regions.
authors.affiliation: Faculty of Medicine, American University of Beirut, Riad El Solh, Beirut 1107-2020, Lebanon. aj00@aub.edu.lb
authors.forename: Abdo
authors.initials: A
authors.lastname: Jurjus
authors.forename: Kassem
authors.initials: K
authors.lastname: Barada
authors.forename: Naim
authors.initials: N
authors.lastname: Khoury
authors.forename: Mona Diab
authors.initials: MD
authors.lastname: Assef
authors.forename: Charlotte Jourdainne
authors.initials: CJ
authors.lastname: Foltzer
authors.forename: Jean Marie
authors.initials: JM
authors.lastname: Reimund
authors.forename: Michele
authors.initials: M
authors.lastname: Kedinger
mesh_terms: D000626:Aminopeptidases / Q000378:metabolism; D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000201:enzymology* / Q000473:pathology*; D003106:Colon / Q000201:enzymology* / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D051296:Glucose Transporter Type 5 / Q000378:metabolism; D007150:Immunohistochemistry; D007460:Iodoacetamide; D007583:Jejunum / Q000201:enzymology / Q000473:pathology*; D043322:Lactase / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012720:Severity of Illness Index; D013393:Sucrase / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
chemical_list: D051296:Glucose Transporter Type 5; D012333:RNA, Messenger; D043322:Lactase; D013393:Sucrase; D000626:Aminopeptidases; D007460:Iodoacetamide