Hydroxymethyl methacrylate-based monolithic columns designed for separation of oligonucleotides in hydrophilic-interaction capillary liquid chromatography.
Hydroxymethyl methacrylate-based monolithic columns for separation of oligonucleotides by capillary liquid chromatography (CLC) were prepared. We optimized composition of the polymerization mixture, which contained the monomer mixture consisting of N-(hydroxymethyl) methacrylamide (HMMAA) and ethylene dimethacrylate (EDMA), and the porogenic system composed of propane-1-ol, butane-1,4-diol and alpha, alpha'-azoisobutyronitrile (AIBN) as initiator. Separations of oligonucleotides were performed in HILIC (hydrophilic-interaction) mode using 100 mM triethylamine acetate (TEAA) in acetonitrile and in water as eluents. The influence of steepness of the mobile phase gradient on separation of the oligonucleotides was evaluated as well as the reproducibility of HMMAA monolith preparation.
Holdsvendová
Petra
P
Charles University in Prague, Faculty of Science, Department of Analytical Chemistry, Albertov 2030, 128 40 Prague 2, Czech Republic.
Suchánková
Jana
J
Buncek
Martin
M
Backovská
Veronika
V
Coufal
Pavel
P
D002853:Chromatography, Liquid / Q000379:methods*; D008768:Methylmethacrylates; D009841:Oligonucleotides / Q000737:chemistry / Q000302:isolation & purification*
D008768:Methylmethacrylates; D009841:Oligonucleotides; C021302:hydroxymethylmethacrylate