A battery of DNA effect biomarkers to evaluate environmental exposure of Flemish adolescents.
The present paper deals with the evaluation of a battery of genotoxicity biomarkers in healthy Flemish adolescents and their relation with common pollutants occurring in their life environment. DNA damage as reflected by the comet assay appeared to be most sensitive to ozone (partial r(2) = 0.102, p < 0.00001), and to a lesser extent to ortho-cresol (partial r(2) = 0.055; p = 0.001) and 1-hydroxy-pyrene (1-OH-pyrene, partial r(2) = 0.031; p = 0.013). 8-hydroxy-deoxyguanosine (8-OHdG) was only related to ortho-cresol (r(2) = 0.069; p < 0.007). Interestingly, the comet assay results and urinary 8-OHdG concentrations were positively correlated with a Pearson r = 0.21 (p = 0.003, N = 200). Logistic regression models revealed significant relations between chromatid breaks and 1-OH-pyrene (relative risk (RR): 1.58; p = 0.008), and t,t-muconic acid (RR: 1.71; p = 0.014). There was no correlation between micronucleus formation or occurrence of chromosomal or chromatid breaks on the one hand and comet or 8-OHdG results on the other hand. Thus, in this study the comet assay on whole blood samples and urine 8-OHdG measurements especially appeared sensitive biomarkers for assessing the genetic effects of environmental pollutants to which adolescents may be exposed.
Koppen
G
G
Center of Expertise in Environmental Toxicology, Flemish Institute of Technological Research (VITO), Mol, Belgium.
Verheyen
G
G
Maes
A
A
Van Gorp
U
U
Schoeters
G
G
Hond
E Den
ED
Staessen
J
J
Nawrot
T
T
Roels
H A
HA
Vlietinck
R
R
Verschaeve
L
L
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000293:Adolescent; D001530:Belgium; D015415:Biomarkers / Q000032:analysis* / Q000097:blood / Q000652:urine; D002869:Chromosome Aberrations; D020552:Comet Assay / Q000379:methods; D003404:Creatinine / Q000652:urine; D003408:Cresols / Q000737:chemistry / Q000652:urine; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine; D004781:Environmental Exposure / Q000032:analysis*; D004785:Environmental Pollutants / Q000032:analysis / Q000097:blood / Q000652:urine; D000431:Ethanol / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D048629:Micronuclei, Chromosome-Defective; D018977:Micronutrients / Q000097:blood; D011721:Pyrenes / Q000032:analysis; D012643:Selenium / Q000097:blood; D012737:Sex Factors; D013011:Sorbic Acid / Q000031:analogs & derivatives / Q000032:analysis; D014801:Vitamin A / Q000097:blood; D014810:Vitamin E / Q000097:blood
D015415:Biomarkers; D003408:Cresols; D004785:Environmental Pollutants; D018977:Micronutrients; D011721:Pyrenes; D014801:Vitamin A; D014810:Vitamin E; D000431:Ethanol; C005939:muconic acid; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003404:Creatinine; D003849:Deoxyguanosine; D012643:Selenium; C033146:1-hydroxypyrene; D013011:Sorbic Acid; C034047:2-cresol