Screening of some medicinal plants from cameroon for beta-lactamase inhibitory activity.
In efforts to find new bioactive beta-lactamase inhibitors, this study investigated 16 Cameroonian plants belonging to 10 families which were evaluated for anti-beta-lactamase activity. The investigation showed that extracts 2, 6, 3 and 5 of the 16 plants investigated presented interesting in vitro beta-lactamase inhibition (over 90%), respectively, of the beta-lactamases TEM-1, OXA-10, IMP-1 and P99. These extracts were from Mammea africana (all beta-lactamases), Garcinia lucida, G. kola (OXA-10, IMP-1 and P99), Bridelia micrantha (OXA-10, P99), Ochna afzelii (OXA-10, P99), Prunus africana (IMP-1) and Adenia lobata (TEM-1). After elimination of tannins (according to the European Pharmacopoeia) the extracts from B. micrantha, G. lucida and M. africana were tested further for their anti-beta-lactamase activity. The extracts from B. micrantha and G. lucida exhibited potent inhibitory activity, respectively, of beta-lactamase OXA-10 (IC(50) = 0.02 mg/mL) and P99 (IC(50) = 0.01 mg/mL). The anti-beta-lactamase activity of M. africana extract was weak. The isolation and the structural elucidation of the active constituents of G. lucida and B. micrantha will provide useful leads in the development of beta-lactamase inhibitors.
Gangoué-Piéboji
Joseph
J
Centre for Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plant Studies, P.O. Box 8404, Yaoundé, Cameroon. jgangoue@yahoo.fr
Baurin
Stéphane
S
Frère
Jean-Marie
JM
Ngassam
Pierre
P
Ngameni
Bathelemy
B
Azebaze
Anatole
A
Pegnyemb
Dieudonné Emmanuel
DE
Watchueng
Jean
J
Goffin
Colette
C
Galleni
Moreno
M
D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D001419:Bacteria / Q000201:enzymology*; D002163:Cameroon; D004791:Enzyme Inhibitors / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D005638:Fruit; D006801:Humans; D020128:Inhibitory Concentration 50; D008519:Medicine, Traditional; D008517:Phytotherapy; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D018517:Plant Roots; D010946:Plants, Medicinal; D012639:Seeds; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases / Q000096:biosynthesis*
D000900:Anti-Bacterial Agents; D004791:Enzyme Inhibitors; D010936:Plant Extracts; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases