Hydrogen peroxide-induced chlorophyll a bleaching in the cytochrome b6f complex: a simple and effective assay for stability of the complex in detergent solutions.
The instability of cytochrome b ( 6 ) f complex in detergent solutions is a well-known problem that has been studied extensively, but without finding a satisfactory solution. One of the important reasons can be short of the useful method to verify whether the complex suspended in different detergent is in an intact state or not. In this article, a simple and effective assay for stability of the complex was proposed based on the investigation on the different effects of the two detergents, n-octyl-beta-D: -glucopyranoside (OG) and dodecyl-beta-D: -maltoside (DDM), on the properties of the complex. DDM stabilizes the complex preparation more effectively whereas OG denatures the interactions of the heme groups and pigment molecules with the protein environment, leading to the bleaching of chlorophyll a induced by addition of hydrogen peroxide. The assay of the use of hydrogen peroxide to characterize the complex by studying the bleaching of chlorophyll induced by hydrogen peroxide and the peroxidase activity of the complex was discussed. This simple method will probably be useful to study the stability of the complex.
Chen
Xiao-Bo
XB
Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Nan Xincun 20, Xiangshan, 100093, Beijing, P.R. China.
Zhao
Xiao-Hui
XH
Zhu
Yi
Y
Gong
Yan-Dao
YD
Li
Liang-Bi
LB
Zhang
Jian-Ping
JP
Kuang
Ting-Yun
TY
D002734:Chlorophyll / Q000737:chemistry*; D000077194:Chlorophyll A; D002942:Circular Dichroism; D045346:Cytochrome b6f Complex / Q000737:chemistry*; D004583:Electrons; D005960:Glucosides / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D007700:Kinetics; D038761:Photobleaching; D013053:Spectrophotometry
D005960:Glucosides; D002734:Chlorophyll; C018619:octyl-beta-D-glucoside; C040358:dodecyl maltoside; D045346:Cytochrome b6f Complex; D006861:Hydrogen Peroxide; D000077194:Chlorophyll A