title: Taxol-resistance-associated gene-3 (TRAG-3/CSAG2) expression is predictive for clinical outcome in ovarian carcinoma patients.
abstract: An obstacle in chemotherapy of ovarian cancer is the development of drug resistance. Taxol (paclitaxel)-resistance-associated gene-3 (TRAG-3/CSAG2) was found to be overexpressed in a paclitaxel-resistant ovarian carcinoma cell line. However, clinical impact of TRAG-3 in ovarian carcinoma has not been demonstrated previously. For demonstration of potential clinical impact of TRAG-3, immunohistochemistry was applied to determine TRAG-3 protein expression in specimens obtained from ovarian carcinoma patients (n=37) who received a paclitaxel-based chemotherapy at two different time points, initial laparotomy before chemotherapy, and secondary cytoreduction after chemotherapy. The TRAG-3-specific immunohistochemical staining was correlated with clinical outcome. In ovarian carcinoma specimens obtained at the initial laparotomy, an advantage in overall (P < 0.001) and progression-free (P = 0.003) survival for patients with weak TRAG-3 expression could be demonstrated. Tumor specimens excised at secondary cytoreduction procedure were not predictive for clinical outcome. In summary, TRAG-3 was found to be a prognostic factor for the prediction of clinical outcome after the application of paclitaxel-based chemotherapy.
authors.affiliation: Institute of Pathology, Charité Campus Mitte, Charitéplatz 1, 10117 Berlin, Germany.
authors.forename: Verena
authors.initials: V
authors.lastname: Materna
authors.forename: Paweł
authors.initials: P
authors.lastname: Surowiak
authors.forename: Irina
authors.initials: I
authors.lastname: Kaplenko
authors.forename: Merek
authors.initials: M
authors.lastname: Spaczyński
authors.forename: Zhenfeng
authors.initials: Z
authors.lastname: Duan
authors.forename: Maciej
authors.initials: M
authors.lastname: Zabel
authors.forename: Manfred
authors.initials: M
authors.lastname: Dietel
authors.forename: Hermann
authors.initials: H
authors.lastname: Lage
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis* / Q000235:genetics; D010051:Ovarian Neoplasms / Q000737:chemistry* / Q000401:mortality; D015996:Survival Rate
chemical_list: C118809:CSAG2 protein, human; D009363:Neoplasm Proteins