title: Isolation of Leptospira noguchii from sheep.
abstract: The main goal of this study was to obtain new isolates of Leptospira spp. from sheep. A total of 10 kidney samples and 44 blood samples were collected from sheep slaughtered in Pelotas, Southern Brazil. One isolate was obtained which was identified by 16S rRNA gene sequencing and serogrouping to be Leptospira noguchii serogroup Autumnalis. Microscopic agglutination test (MAT) evaluation revealed that 4.5% of the sheep sera reacted against the Autumnalis serogroup. This is the first report of isolation of L. noguchii from sheep. Together these findings indicate that L. noguchii infections may be a potentially important veterinary problem in this domestic animal species.
authors.affiliation: Centro de Controle de Zoonoses, Faculdade de Medicina Veterinária, Brazil. efsilva@ufpel.edu.br
authors.forename: Everton F
authors.initials: EF
authors.lastname: Silva
authors.forename: Claudiomar S
authors.initials: CS
authors.lastname: Brod
authors.forename: Gustavo M
authors.initials: GM
authors.lastname: Cerqueira
authors.forename: Débora
authors.initials: D
authors.lastname: Bourscheidt
authors.forename: Núbia
authors.initials: N
authors.lastname: Seyffert
authors.forename: Adriano
authors.initials: A
authors.lastname: Queiroz
authors.forename: Cleiton S
authors.initials: CS
authors.lastname: Santos
authors.forename: Albert I
authors.initials: AI
authors.lastname: Ko
authors.forename: Odir A
authors.initials: OA
authors.lastname: Dellagostin
mesh_terms: D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001483:Base Sequence; D006224:Cricetinae; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D007674:Kidney Diseases / Q000097:blood / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D007919:Leptospira / Q000235:genetics / Q000302:isolation & purification*; D007922:Leptospirosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology / Q000382:microbiology*
chemical_list: D000907:Antibodies, Bacterial; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S