The sugar phosphotransferase system of Vibrio fischeri inhibits both motility and bioluminescence.
Magnesium-dependent induction of Vibrio fischeri flagellar (Mif) biogenesis depends upon two diguanylate cyclases, suggesting an inhibitory role for cyclic di-GMP. Here, we report that cells defective for the sugar phosphotransferase system (PTS) exhibited a magnesium-independent phenotype similar to that of mutants of the Mif pathway. Unlike Mif mutants, PTS mutants also were hyperbioluminescent.
Visick
Karen L
KL
Department of Microbiology and Immunology, Loyola University Medical School, 2160 S. First Ave., Bldg. 105, Rm. 3846, Maywood, IL 60153, USA.
O'Shea
Therese M
TM
Klein
Adam H
AH
Geszvain
Kati
K
Wolfe
Alan J
AJ
D048908:Aliivibrio fischeri / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins; D005407:Flagella / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial; D049449:Luminescence; D008274:Magnesium / Q000378:metabolism; D009068:Movement / Q000502:physiology*; D009154:Mutation; D010641:Phenotype; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics / Q000378:metabolism*; D019761:Phosphorus-Oxygen Lyases / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D019761:Phosphorus-Oxygen Lyases; C060720:diguanylate cyclase; D008274:Magnesium