Cyclin E expression and chemotherapeutic sensitivity in breast cancer cells.
The effects of the cyclin E expression levels on chemotherapeutic sensitivity of breast cancer cell line were explored. After the cyclin E expression was knockdown in MDA-MB-435 by RNA interference, FACS analysis and SA-beta-gal staining were used to evaluate the response sensitivity of breast cancer cells to DNA damage drugs (adriamycin, etc.). Adriamycin could induce G1 arrest in cyclin E knockdown MDA-MB-435 breast cell line and increase the percentage of cell senescence in cyclin E knockdown MDA-MB-435 cells. It was suggested that cyclin E knockdown could increase the chemotherapeutic sensitivity of breast cancer cells to DNA damage drugs.
Chen
Jianying
J
Department of General Surgery, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, China.
Wang
Guobin
G
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D016922:Cellular Senescence; D019927:Cyclin E / Q000235:genetics / Q000378:metabolism*; D004249:DNA Damage / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology; D055785:Gene Knockdown Techniques; D006801:Humans; D015513:Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference
D000903:Antibiotics, Antineoplastic; C475820:CCNE1 protein, human; D019927:Cyclin E; D015513:Oncogene Proteins; D004317:Doxorubicin