title: Identification of a soluble NADPH oxidoreductase (BmNOX) with antiviral activities in the gut juice of Bombyx mori.
abstract: Silkworms show high variability in silk quality and disease resistance. Attempts are on to combine the disease tolerance of multivoltine races and the silk quality of bivoltine races to generate new races with desirable phenotypic traits. We report the identification of a 26.5-kDa protein that is overexpressed in the gut juice of disease-resistant multivoltine races and that has anti-BmNPV activity. We have characterized this protein as a soluble NADH-oxidoreductase-like protein (BmNOX). Treatment of live BmNPV particles with BmNOX inhibited the capability of the viral particles to infect BmN cells in vitro.
authors.affiliation: Central Sericultural Research and Training Institute, Mysore, India.
authors.forename: Ruchita
authors.initials: R
authors.lastname: Selot
authors.forename: Virendra
authors.initials: V
authors.lastname: Kumar
authors.forename: Sunita
authors.initials: S
authors.lastname: Shukla
authors.forename: Kumar
authors.initials: K
authors.lastname: Chandrakuntal
authors.forename: Mopidevi
authors.initials: M
authors.lastname: Brahmaraju
authors.forename: S B
authors.initials: SB
authors.lastname: Dandin
authors.forename: Malini
authors.initials: M
authors.lastname: Laloraya
authors.forename: Pradeep G
authors.initials: PG
authors.lastname: Kumar
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology*; D012831:Bombyx / Q000201:enzymology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D005750:Gastric Juice / Q000201:enzymology*; D008970:Molecular Weight; D009247:NADH, NADPH Oxidoreductases / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology*; D012995:Solubility
chemical_list: D000998:Antiviral Agents; D009247:NADH, NADPH Oxidoreductases