title: [Study on purification of Radix Paeoniae Alba by HPD100 macroporous resin].
abstract: OBJECTIVE
  To set up the method for purification of paeoniflorin by HPD100 macroporous resin.
  
  
  METHODS
  With the adsorption ration and desorption rate of paeoniflorin as the evaluating quotas, the single factor test, orhogonal experimental designs and variance analysis were applied to optimize the manipulation parameters of macrporous resin.
  
  
  RESULTS
  The HPD100 had the best adsorbive and separative properties to paeoniflorin. The applicable technology was as follows: the sample concentration was 0.5 g/ml, the maximum capacity for medicinal materials was 10 g/g, the current velocit was 2 BV/h, the eluting reagent was 30% ethanol (4 times the volume of bottle).
  
  
  CONCLUSIONS
  HPD100 resin can be used in purify of Radix Paeoniae Alba successfully. The purity of paeoniflorin was above 30%.
authors.affiliation: Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Ya-jun
authors.initials: YJ
authors.lastname: Zhang
authors.forename: Lian-ying
authors.initials: LY
authors.lastname: Xu
mesh_terms: D000327:Adsorption; D001565:Benzoates / Q000032:analysis / Q000302:isolation & purification*; D001952:Bridged-Ring Compounds / Q000032:analysis / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D000431:Ethanol; D005960:Glucosides / Q000032:analysis / Q000302:isolation & purification*; D039821:Monoterpenes; D029596:Paeonia / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012117:Resins, Synthetic; D013678:Technology, Pharmaceutical / Q000379:methods*; D006027:Glycosides
chemical_list: D001565:Benzoates; D001952:Bridged-Ring Compounds; D004365:Drugs, Chinese Herbal; D000431:Ethanol; D005960:Glucosides; D039821:Monoterpenes; D012117:Resins, Synthetic; C015423:peoniflorin; D006027:Glycosides