title: The effect of centaurein on interferon-gamma expression and Listeria infection in mice.
abstract: We previously found that centaurein enhanced IFN-gamma transcription in T cells. Here, we demonstrate that centaurein increased the IFN-gamma expression in T and NK cells and the serum IFN-gamma level in mice. Centaurein elevated the transcription of T-bet but not GATA-3, which is consistent with its effect on that of IFN-gamma but not IL-4. Additionally, centaurein effectively protected mice against Listeria infection. Moreover, centaurein per se or in combination with antibiotics could treat Listeria infection. Our mechanistic studies suggest that centaurein augments IFN-gamma expression via a transcriptional up-regulation of T-bet and that centaurein protects against or treats Listeria infection via a modulation of IFN-gamma expression.
authors.affiliation: Agricultural Biotechnology Research Center, 128, Sec. 2, Academia Road, Nankang, Taipei 115, Taiwan.
authors.forename: Shu-Lin
authors.initials: SL
authors.lastname: Chang
authors.forename: Hsu-Hua
authors.initials: HH
authors.lastname: Yeh
authors.forename: Yu-Shiun
authors.initials: YS
authors.lastname: Lin
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Chiang
authors.forename: Tung-Kung
authors.initials: TK
authors.lastname: Wu
authors.forename: Wen-Chin
authors.initials: WC
authors.lastname: Yang
mesh_terms: D000818:Animals; D031176:Bidens / Q000737:chemistry; D018274:Electroporation; D005419:Flavonoids / Q000494:pharmacology*; D005960:Glucosides / Q000494:pharmacology*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D007694:Killer Cells, Natural / Q000187:drug effects / Q000378:metabolism; D008088:Listeriosis / Q000188:drug therapy* / Q000382:microbiology; D008156:Luciferases / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008519:Medicine, Traditional; D051379:Mice; D008810:Mice, Inbred C57BL; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D013268:Stimulation, Chemical; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism
chemical_list: D005419:Flavonoids; D005960:Glucosides; C111521:centaurein; D007371:Interferon-gamma; D008156:Luciferases