Gender and age differences in expression of GABAA receptor subunits in rat somatosensory thalamus and cortex in an absence epilepsy model.
Absence epilepsy is more prevalent in females, but reasons for this gender asymmetry are unknown. We reported previously that perinatal treatment of Long-Evans Hooded rats with the cholesterol synthesis inhibitor (CSI) AY9944 causes a life-long increase in EEG spike-wave discharges (SWDs), correlated with decreased expression of GABA(A) receptor subunit gamma2 protein levels in thalamic reticular and ventrobasal nuclei (SS thalamus) [Li, H., Kraus, A., Wu, J., Huguenard, J.R., Fisher, R.S., 2006. Selective changes in thalamic and cortical GABA(A) receptor subunits in a model of acquired absence epilepsy in the rat. Neuropharmacology 51, 121-128]. In this study, we explored time course and gender different effects of perinatal AY9944 treatment on expression of GABA(A) receptor alpha1 and gamma2 subunits in SS thalamus and SS cortex. Perinatal AY9944 treatment-induced decreases in GABA(A) gamma2 receptor subunits in rat SS thalamus and increases in SS cortex are gender and age specific. The findings suggest a mechanism for the higher prevalence of absence epilepsy in female patients.
Li
Huifang
H
Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Room A343, Stanford Medical Center, 300 Pasteur Drive, Stanford, CA 94305-5235, USA. huifang@stanford.edu
Huguenard
John R
JR
Fisher
Robert S
RS
D000367:Age Factors; D000818:Animals; D000924:Anticholesteremic Agents; D004195:Disease Models, Animal; D004832:Epilepsy, Absence / Q000139:chemically induced / Q000378:metabolism / Q000503:physiopathology*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D020646:Intralaminar Thalamic Nuclei / Q000254:growth & development / Q000502:physiology*; D008297:Male; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D020318:Rats, Long-Evans; D011963:Receptors, GABA-A / Q000235:genetics* / Q000378:metabolism; D012727:Sex Characteristics; D013003:Somatosensory Cortex / Q000254:growth & development / Q000502:physiology*; D020651:Ventral Thalamic Nuclei / Q000254:growth & development / Q000502:physiology*; D001371:trans-1,4-Bis(2-chlorobenzaminomethyl)cyclohexane Dihydrochloride
D000924:Anticholesteremic Agents; D021122:Protein Subunits; D012333:RNA, Messenger; D011963:Receptors, GABA-A; D001371:trans-1,4-Bis(2-chlorobenzaminomethyl)cyclohexane Dihydrochloride