Ricin antitoxins based on lyotropic mesophases containing galactose amphiphiles.
Lyotropic mesophases of the inverse hexagonal or cubic type are nanostructured materials that result from the self-assembly of amphiphilic surfactant molecules in water. The extremely large area of the surfactant-water interface inherent within these structures makes them attractive media for sorbent or encapsulant systems. Here, we report on the development of a new class of polyvalent materials that are based on the incorporation of bioactive ligands within lyotropic mesophases. In particular, we have studied the potential for these materials to behave as polyvalent antitoxins by incorporating synthetic galactose amphiphiles, which mimic the natural cell surface ligand for the protein toxin ricin. The study demonstrates that cubic morphology lyotropic mesophases containing galactose amphiphiles exhibit high specificity ricin uptake, with favorably high dissociation constants and high capacities. We suggest that lyotropic mesophase polyvalent ligands are thus promising materials for the incorporation of a broad range of cell surface recognition moieties and hence may have wide applicability as materials capable of partaking in biological recognition processes.
Hartley
P G
PG
CSIRO Molecular & Health Technologies, Private Bag 10, Clayton South, Victoria 3169, Australia. patrick.hartley@csiro.au
Alderton
M R
MR
Dawson
R M
RM
Wells
D
D
D000992:Antitoxins / Q000737:chemistry*; D005690:Galactose / Q000737:chemistry*; D015394:Molecular Structure; D012276:Ricin / Q000737:chemistry*; D012996:Solutions; D013501:Surface-Active Agents / Q000737:chemistry*
D000992:Antitoxins; D012996:Solutions; D013501:Surface-Active Agents; D012276:Ricin; D005690:Galactose