An enzymatic method for site-specific labeling of recombinant proteins with oligonucleotides.
DNA was site-specifically conjugated to a substrate peptide of microbial transglutaminase fused to the N- or C-terminus of target proteins without the loss of the proteins' functions of interest.
Tominaga
Jo
J
Department of Applied Chemistry, Graduate School of Engineering, 744 Motooka, Fukuoka, 819-0395, Japan.
Kemori
Yoshinori
Y
Tanaka
Yusuke
Y
Maruyama
Tatsuo
T
Kamiya
Noriho
N
Goto
Masahiro
M
D000595:Amino Acid Sequence; D009841:Oligonucleotides / Q000737:chemistry* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D011503:Transglutaminases / Q000378:metabolism*
D009841:Oligonucleotides; D011994:Recombinant Proteins; D011503:Transglutaminases