DNA-methyltransferase SsoII as a bifunctional protein: features of the interaction with the promoter region of SsoII restriction-modification genes.
DNA duplexes bearing an aldehyde group at the 2'-position of the sugar moiety were used for affinity modification of (cytosine-5)-DNA methyltransferase SsoII. It is shown that lysine residues of M.SsoII N-terminal region are located in proximity to DNA sugar-phosphate backbone of a regulatory sequence of promoter region of SsoII restriction-modification enzyme coding genes. The ability of the two M.SsoII subunits to interact with DNA regulatory sequence has been demonstrated by affinity modification using DNA duplexes with two 2'-aldehyde groups. Changes in nucleotide sequence of one half of the regulatory region prevented cross-linking of the second M.SsoII subunit. The results on sequential affinity modification of M.SsoII by two types of modified DNA ligands (i.e. by 2'-aldehyde-containing and phosphoryldisulfide-containing) have demonstrated the possibility of covalent attachment of the protein to two different DNA recognition sites: regulatory sequence and methylation site.
Romanenkov
A S
AS
Chemical Faculty, Lomonosov Moscow State University, Moscow, 119992, Russia.
Kisil
O V
OV
Zatsepin
T S
TS
Iamskova
O V
OV
Kariagina
A S
AS
Metelev
V G
VG
Oretskaia
T S
TS
Kubareva
E A
EA
D020134:Catalytic Domain; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D015280:DNA Restriction-Modification Enzymes / Q000737:chemistry* / Q000378:metabolism; D015257:DNA-Cytosine Methylases / Q000737:chemistry* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding
D015280:DNA Restriction-Modification Enzymes; D004247:DNA; C063974:DNA modification methylase SsoII; D015257:DNA-Cytosine Methylases