title: New advances in fluorochrome sequential labelling of teeth using seven different fluorochromes and spectral image analysis.
abstract: Fluorochrome sequential labelling of mineralizing tissues is commonly used in different fields of clinical and basic research. Recently we improved polychrome fluorescent sequential labelling of bone by applying spectral image analysis to discriminate seven different fluorochromes. Although basic mineralization processes of bone and teeth follow comparable principles, the respective tissues differ in terms of matrix composition and mineral assembly. The aim of this study therefore was to investigate the feasibility of this new technique for polychrome sequential labelling of teeth and to demonstrate the advantages in the field of dentistry. Furthermore, the exact labelled area of each fluorochrome could be measured, even in regions of overlapping fluorochromes. The technique presented may provide a basis for further investigations of mineralization processes of different anatomical dental structures.
authors.affiliation: Department of Oral and Cranio-Maxillofacial Surgery, Technical University of Munich, Germany. christoph.pautke@gmx.net
authors.forename: Christoph
authors.initials: C
authors.lastname: Pautke
authors.forename: Thomas
authors.initials: T
authors.lastname: Tischer
authors.forename: Stephan
authors.initials: S
authors.lastname: Vogt
authors.forename: Cornelia
authors.initials: C
authors.lastname: Haczek
authors.forename: Herbert
authors.initials: H
authors.lastname: Deppe
authors.forename: Andreas
authors.initials: A
authors.lastname: Neff
authors.forename: Hans-Henning
authors.initials: HH
authors.lastname: Horch
authors.forename: Matthias
authors.initials: M
authors.lastname: Schieker
authors.forename: Andreas
authors.initials: A
authors.lastname: Kolk
mesh_terms: D000818:Animals; D005456:Fluorescent Dyes; D007091:Image Processing, Computer-Assisted; D008297:Male; D008856:Microscopy, Fluorescence / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D013053:Spectrophotometry; D014070:Tooth / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes