[Effects of Dengue virus infection on PGIS expression and PGI2 secretion of human vascular endothelial cells].
OBJECTIVE
To elucidate the effects of Dengue virus on the expression and secretion of prostacyclin (PGI(2)) in endothelial cells.


METHODS
Cultured human umbilical vein endothelial cells (HUVEC) were infected by Dengue virus II(DV(2)) for different duration. PGI(2) level in the supernatant was determined by radioimmune assay. Cytoplasmic RNA of the infected HUVEC was prepared using the Trizol method and was assayed for prostacyclin synthase (PGIS) by RT-PCR.


RESULTS
PGIS expression as well as PGI(2) secretion of the DV(2) group were significantly increased (P<0.05) at 48 h, 72 h and 96 h post-infection compared to the control group.


CONCLUSIONS
DV(2) could promote the expression of PGIS mRNA in HUVEC and increase the level of PGI(2), which may increase the vascular permeability. The dysfunction of vascular endothelial cell (EC) induced by DV may be related to the pathogenesis of Dengue haemorrhagic fever (DHF) and Dengue shock syndrome(DSS).
Jiang
Zhen-you
ZY
Department of Microbiology and Immunology, Medical College of Jinan University, Guangzhou 510632, China. tjzhy@jnu.edu.cn
Xiao
Rui
R
D002478:Cells, Cultured; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D003715:Dengue / Q000378:metabolism / Q000503:physiopathology*; D003716:Dengue Virus / Q000502:physiology*; D042783:Endothelial Cells / Q000378:metabolism* / Q000821:virology*; D011464:Epoprostenol / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D003577:Cytochrome P-450 Enzyme System; D011464:Epoprostenol; D019746:Intramolecular Oxidoreductases; C022461:prostacyclin synthetase