Distribution and characterization of nitric oxide synthase in the nervous system of Triatoma infestans (Insecta: Heteroptera).
The biochemical characterization of nitric oxide synthase (NOS) and its distribution in the central nervous system (CNS) were studied in the heteropteran bug Triatoma infestans. NOS-like immunoreactivity was found in the brain, subesophageal ganglion, and thoracic ganglia by using immunocytochemistry. In the protocerebrum, NOS-immunoreactive (IR) somata were detected in the anterior, lateral, and posterior soma rinds. In the optic lobe, numerous immunostained somata were observed at the level of the first optic chiasma, around the lobula, and in the proximal optic lobe. In the deutocerebrum, NOS-IR perikarya were mainly observed in the lateral soma rind, surrounding the sensory glomeruli, and a few cell bodies were seen in association with the antennal mechanosensory and motor neuropil. No immunostaining could be detected in the antennal nerve. The subesophageal and prothoracic ganglia contained scattered immunostained cell bodies. NOS-IR somata were present in all the neuromeres of the posterior ganglion. Western blotting showed that a universal NOS antiserum recognized a band at 134 kDa, in agreement with the expected molecular weight of the protein. Analysis of the kinetics of nitric oxide production revealed a fully active enzyme in tissue samples of the CNS of T. infestans.
Settembrini
Beatriz P
BP
Facultad de Ciencias Biomédicas, Universidad Austral, Pte Perón 1500, B1629AHJ, Pilar, Pcia de Buenos Aires, Argentina. settembrini@macn.gov.ar
Coronel
María F
MF
Nowicki
Susana
S
Nighorn
Alan J
AJ
Villar
Marcelo J
MJ
D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000201:enzymology; D017952:Ganglia, Invertebrate / Q000201:enzymology; D007700:Kinetics; D008297:Male; D009412:Nerve Fibers / Q000201:enzymology; D009420:Nervous System / Q000166:cytology / Q000201:enzymology*; D009569:Nitric Oxide / Q000096:biosynthesis; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D021381:Protein Transport; D014224:Triatoma / Q000201:enzymology*
D009569:Nitric Oxide; D019001:Nitric Oxide Synthase