Structural characterization of a glucan isolated from Hedyotis diffusa Willd.
OBJECTIVE
To isolate a homogeneous polysaccharide RP from RDP (a crude polysaccharide from Dioscorea opposita Thunb.), and study its preliminary com-position and structure.


METHODS
RP was obtained with water extraction, alcohol sedimentation, CTAB deprotein, cellulose column and SephadexG-100 column. The purities of RP were identified by SephadexG-200. PC analysis on its acidic hydrolysates was used to determine the sugar components. Sephadex chromatography was used to mensurate its molecular weight. IR was used to analysis RP.


RESULTS
RP was homogeneous. IR indicated that RP had P configurations. The compositon was identified by paper chromatography as glucose, D-mannose, D-galactose.


CONCLUSIONS
The research could provide a theoretical foundation for further development and utilization.
Cui
Jian
J
Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Wang
Shun-chun
SC
Shi
Song-shan
SS
Wang
Zheng-tao
ZT
D005936:Glucans / Q000737:chemistry* / Q000302:isolation & purification*; D005947:Glucose / Q000737:chemistry; D032064:Hedyotis / Q000737:chemistry*; D015394:Molecular Structure; D008970:Molecular Weight; D010946:Plants, Medicinal / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
D005936:Glucans; D005947:Glucose