title: Isolation of two regions on rat chromosomes 5 and 18 affecting mammary cancer susceptibility.
abstract: We previously mapped several quantitative trait loci (QTLs) controlling DMBA-induced mammary tumor development in female rats derived from a SPRD-Cu3 (susceptible strain) x WKY (resistant strain) cross. Two of these QTLs were assigned to chromosomes 5 and 18. In the present study, we generated and characterized congenic strains in which a segment of WKY chromosomes 5 or 18 was introduced in the SPRD-Cu3 genetic background, thereby physically demonstrating that each of these two chromosomes controls mammary tumor multiplicity. The chromosome 5 QTL (Mcstm1) accounts for 7 tumors per animal (versus a total of 11 tumors per SPRD-Cu3 rat). The chromosome 18 QTL (Mcstm2) accounts for 3 tumors per animal and is the first chemically-induced mammary cancer susceptibility locus assigned to this chromosome. In addition, the Mcstm1 region was shown to also controls tumor latency. These loci thus play a major role in chemically-induced mammary tumor development. QTLs controlling chemically-induced or estrogen-induced mammary tumor development have independently been identified on chromosomes 5 and 18, using susceptible strains others than SPRD-Cu3. Therefore the haplotype structure of the relevant chromosome regions was analyzed in the different strains. Some chromosome regions were found to be highly mosaic (haplotype blocks < 1 Mb), while one region showed an apparently conserved haplotype block of 7.5 Mb. This analysis points to limited regions that could harbor the causative genes and also indicates that at least Mcstm2 is a novel QTL.
authors.affiliation: Université Libre de Bruxelles, Institut de Biologie et de Médecine Moléculaires, Rue Profs Jeener & Brachet, 12, B-6041 Gosselies (Charleroi), Belgium.
authors.forename: Daniel
authors.initials: D
authors.lastname: Stieber
authors.forename: Géraldine
authors.initials: G
authors.lastname: Piessevaux
authors.forename: Michèle
authors.initials: M
authors.lastname: Rivière
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Laes
authors.forename: Xiaojiang
authors.initials: X
authors.lastname: Quan
authors.forename: Josiane
authors.initials: J
authors.lastname: Szpirer
authors.forename: Claude
authors.initials: C
authors.lastname: Szpirer
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity; D000818:Animals; D020296:Animals, Congenic; D002273:Carcinogens / Q000633:toxicity; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics*; D003433:Crosses, Genetic; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D040641:Quantitative Trait Loci / Q000235:genetics*; D019655:Quantitative Trait, Heritable; D051381:Rats; D011921:Rats, Inbred WKY; D017207:Rats, Sprague-Dawley
chemical_list: D002273:Carcinogens; D015127:9,10-Dimethyl-1,2-benzanthracene