title: Characterization of Plasmodium vivax heat shock protein 70 and evaluation of its value for serodiagnosis of tertian malaria.
abstract: We have characterized Plasmodium vivax heat shock protein 70 (PvHSP70) and evaluated serodiagnostic applicability of recombinant PvHSP70 (rPvHSP70). In enzyme-linked immunosorbent assays and immunoblot analyses, rPvHSP70 showed high sensitivity (88.8%; 203/228 cases). P. falciparum-infected sera revealed positive reactions (78.8%). The predominant immunoglobulin G (IgG) subclasses were segregated with IgG1 and IgG3.
authors.affiliation: Department of Molecular Parasitology, Sungkyunkwan University School of Medicine, Suwon 440-746, Korea.
authors.forename: Byoung-Kuk
authors.initials: BK
authors.lastname: Na
authors.forename: Jae-Won
authors.initials: JW
authors.lastname: Park
authors.forename: Hyeong-Woo
authors.initials: HW
authors.lastname: Lee
authors.forename: Klin
authors.initials: K
authors.lastname: Lin
authors.forename: Seon-Hee
authors.initials: SH
authors.lastname: Kim
authors.forename: Young-An
authors.initials: YA
authors.lastname: Bae
authors.forename: Woon-Mok
authors.initials: WM
authors.lastname: Sohn
authors.forename: Tong-Soo
authors.initials: TS
authors.lastname: Kim
authors.forename: Yoon
authors.initials: Y
authors.lastname: Kong
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D001483:Base Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000145:classification; D008288:Malaria / Q000175:diagnosis*; D008969:Molecular Sequence Data; D010966:Plasmodium vivax / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D012680:Sensitivity and Specificity; D012698:Serologic Tests
chemical_list: D000913:Antibodies, Protozoan; D018840:HSP70 Heat-Shock Proteins; D007074:Immunoglobulin G; D011994:Recombinant Proteins