title: Quantification of active principles and pigments in leaf extracts of Isatis tinctoria by HPLC/UV/MS.
abstract: An HPLC method has been developed and validated for the quantification of the pharmacologically active principles tryptanthrin (1), 1,3-dihydro-3-[(4-hydroxy-3,5-dimethoxyphenyl)methylene]-2 H-indol-2-one (indolinone) (3), indirubin (4), alpha-linolenic acid (2), and indigo (5), an isomer of indirubin, in extracts from the traditional anti-inflammatory plant Isatis tinctoria (woad). The chromatographic separation was performed on a C-18 column with a linear gradient of acetonitrile in water containing 0.1% formic acid. The method combines UV and electrospray MS detection in the positive ion mode for the detection of the alkaloids, with a switch to the negative mode for the analysis of alpha-linolenic acid. The method was applied to the analysis of woad extracts obtained by supercritical fluid (SFE) CO2 extraction, and by pressurized liquid extraction (PLE) with dichloromethane and methanol, respectively. While the highest concentration of alpha-linolenic acid was found in the SFE extract (7.43%), the concentrations of tryptanthrin , indolinone, indirubin and indigo were the highest in the dichloromethane extract (0.30, 0.035, 2.48 and 0.84%, respectively). Compound 3 was not detected in the methanolic extract and only traces of compounds 1, 4 and 5 and low amount of alpha-linolenic acid (0.39%) were present in this extract.
authors.affiliation: Institute of Pharmaceutical Biology, University of Basel, Basel, Switzerland.
authors.forename: Tobias
authors.initials: T
authors.lastname: Mohn
authors.forename: Olivier
authors.initials: O
authors.lastname: Potterat
authors.forename: Matthias
authors.initials: M
authors.lastname: Hamburger
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007203:Indigo Carmine; D007211:Indoles / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D031219:Isatis / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D023421:Models, Animal; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D011799:Quinazolines / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013056:Spectrophotometry, Ultraviolet; D017962:alpha-Linolenic Acid / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000893:Anti-Inflammatory Agents; D007211:Indoles; D010936:Plant Extracts; D011799:Quinazolines; D017962:alpha-Linolenic Acid; C046243:tryptanthrine; D007203:Indigo Carmine; C027185:indirubin