title: Morphologic and histologic comparisons between in vivo and nuclear transfer derived porcine embryos.
abstract: Nuclear transfer (NT) is an inefficient but invaluable tool of the biotechnology industry. This study looked at abnormalities associated with peri-implantation NT porcine embryos. Four experimental groups were examined: nonpregnant animals, in vivo pregnant animals, NT recipients, and manipulation control embryos (MC). Embryos (Day 10, 12, or 14) were evaluated for embryonic disc diameter, gross morphology, nucleoli density, and mitotic figure index. Day 12 (P < or = 0.03) and Day 14 (P < or = 0.01) NT embryos had increased numbers of nucleoli, and Day 14 NT embryos had an increased (P < or = 0.03) mitotic index compared to in vivo and MC embryos. In vivo produced Day 14 embryos had increased (P < or = 0.01) disk diameters when compared to other embryos except for MC Day 14, which also showed increases (P < or = 0.01) in disk diameter except when compared to in vivo produced Day 12 and Day 14 embryos. In vivo produced Day 12 had greater (P < or = 0.03) disk diameters when compared to NT and MC embryos except for MC Day 14, and in vivo produced Day 14 embryos, which had a significantly increased (P < or = 0.01) disk diameter. In vivo produced Day 14 embryos were morphologically more advanced (P < or = 0.01) than Day 14 NT and MC counterparts. NT embryos develop at a slower rate than their in vivo produced counterparts. The increase in nucleoli and mitotic index of NT embryos suggest the cell cycle may be affected or the NT embryos are employing other means to compensate for slow development. The techniques used during NT also appear to compromise embryo development.
authors.affiliation: Division of Animal Sciences, University of Missouri-Columbia, Columbia, MO 65211, USA.
authors.forename: L
authors.initials: L
authors.lastname: Martin
authors.forename: C
authors.initials: C
authors.lastname: Besch-Williford
authors.forename: L
authors.initials: L
authors.lastname: Lai
authors.forename: H-T
authors.initials: HT
authors.lastname: Cheong
authors.forename: G-S
authors.initials: GS
authors.lastname: Im
authors.forename: K-W
authors.initials: KW
authors.lastname: Park
authors.forename: C
authors.initials: C
authors.lastname: Murphy
authors.forename: Y
authors.initials: Y
authors.lastname: Hao
authors.forename: M R
authors.initials: MR
authors.lastname: Ellersieck
authors.forename: D H
authors.initials: DH
authors.lastname: Keisler
authors.forename: H
authors.initials: H
authors.lastname: Schatten
authors.forename: J A
authors.initials: JA
authors.lastname: Green
authors.forename: R S
authors.initials: RS
authors.lastname: Prather
mesh_terms: D000818:Animals; D002466:Cell Nucleolus / Q000648:ultrastructure; D004622:Embryo, Mammalian / Q000166:cytology / Q000502:physiology*; D005260:Female; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D008938:Mitosis; D053652:Nuclear Transfer Techniques; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D011897:Random Allocation; D013552:Swine; D014599:Uterus / Q000033:anatomy & histology
chemical_list: D011374:Progesterone; D007334:Insulin-Like Growth Factor I