title: High level expression of biologically active canine interferon-alpha subtype 4 using a baculovirus.
abstract: In this study, a high amount of bioactive recombinant canine interferon-alpha subtype 4 (CaIFN-alpha4) was expressed in a baculovirus system. For easy purification, it was expressed as a CaIFN-alpha4 bearing histidine hexamer at the C-terminal region, designated CaIFN-alpha4His. CaIFN-alpha4His was detected in culture supernatants of insect cells infected with the recombinant virus using sodium dodecyl sulfate-polyarcylamide gel electrophoresis (SDS-PAGE) and Coomassie Brilliant Blue staining. The level of expression was very high, and approximately 1 mg of purified protein, with 5.0 x 10(7) units/mg, was obtained from 300 ml of culture supernatant. The purified product showed antiviral activity against Vesicular stomatitis virus on canine tumor cell line A72 and chicken embryo fibroblast cells.
authors.affiliation: Laboratory of Zoonoses, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori, Japan.
authors.forename: Ruttapong
authors.initials: R
authors.lastname: Ruttanapumma
authors.forename: Manami
authors.initials: M
authors.lastname: Anzai
authors.forename: Masaoki
authors.initials: M
authors.lastname: Takegawa
authors.forename: Masashi
authors.initials: M
authors.lastname: Okamura
authors.forename: Nobutoshi
authors.initials: N
authors.lastname: Maehara
authors.forename: Ko
authors.initials: K
authors.lastname: Sato
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Yoshioka
authors.forename: Akira
authors.initials: A
authors.lastname: Itoh
authors.forename: Masayuki
authors.initials: M
authors.lastname: Nakamura
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Takehara
mesh_terms: D000818:Animals; D016367:Baculoviridae; D002460:Cell Line; D004285:Dogs; D005786:Gene Expression Regulation; D007313:Insecta; D016898:Interferon-alpha / Q000378:metabolism*
chemical_list: D016898:Interferon-alpha