Peptidomic analysis of human peripheral monocytes persistently infected by Chlamydia trachomatis.
Peptidomic analysis using Differential Peptide Display (DPD) of human peripheral blood mononuclear cells (PBMC) mock-infected or persistently infected by Chlamydia trachomatis (CT) revealed 10 peptides, expressed upon CT infection. Analysis of these 10 candidates by tandem mass spectrometry enabled the determination of seven candidates as fragments from the precursors (I) ferritin heavy chain subunit, (II) HLA class II histocompatibility antigen, (III) vimentin, (IV) indoleamine 2,3-dioxygenase, (V and VI) pre-B cell enhancing factor (PBEF), and (VII) Interleukin-8 (CXCL8). The identified candidates proved the presence of anti-bacterial and immunologically active monocytic proteins after CT infection.
Krausse-Opatz
Birgit
B
Division of Rheumatology, Department of Internal Medicine, Hannover Medical School, Hannover, Germany. krausse-opatz.birgit@mh-hannover.de
Busmann
Annette
A
Tammen
Harald
H
Menzel
Christoph
C
Möhring
Thomas
T
Le Yondre
Nicolas
N
Schmidt
Cornelia
C
Schulz-Knappe
Peter
P
Zeidler
Henning
H
Selle
Hartmut
H
Köhler
Lars
L
D002692:Chlamydia trachomatis / Q000276:immunology* / Q000472:pathogenicity / Q000502:physiology; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D006801:Humans; D009000:Monocytes / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology*; D010455:Peptides / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
D016207:Cytokines; D010455:Peptides