title: Rapid purification and scale-up of honokiol and magnolol using high-capacity high-speed counter-current chromatography.
abstract: In this paper, a rapid separation approach has been developed using high-capacity high-speed counter-current chromatography (high-capacity HSCCC) to isolate and purify honokiol and magnolol, which are the main bioactive constituents from Houpu. The optimization of the solvent selection process, sample loading volume and flow rate is systematically studied using analytical high-capacity HSCCC. The optimized parameters obtained rapidly at analytical scale were used for a 1000 x scale-up preparative run using pilot scale high-capacity HSCCC in a MAXI-DE centrifuge. A crude sample of 43 g was successfully separated and the fractions were analysed by high-performance liquid chromatography (HPLC). This large scale preparative single step run yielded 16.9 and 19.4 g of honokiol and magnolol with purities of 98.6 and 99.9%, in only 20 min. This is the first time that high-performance counter-current chromatography has been used to purify multiple gram grade bioactive compounds in less than 1h and at such high concentrations of final products (10.8 g/l for magnolol and 7.0 g/l for honokiol).
authors.affiliation: State Key laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China. lijuan17@hotmail.com
authors.forename: Lijuan
authors.initials: L
authors.lastname: Chen
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.forename: Guangli
authors.initials: G
authors.lastname: Yang
authors.forename: Linyu
authors.initials: L
authors.lastname: Fan
authors.forename: James
authors.initials: J
authors.lastname: Tang
authors.forename: Ian
authors.initials: I
authors.lastname: Garrard
authors.forename: Svetlana
authors.initials: S
authors.lastname: Ignatova
authors.forename: Derek
authors.initials: D
authors.lastname: Fisher
authors.forename: Ian A
authors.initials: IA
authors.lastname: Sutherland
mesh_terms: D001713:Biphenyl Compounds / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D017705:Lignans / Q000302:isolation & purification*; D010865:Pilot Projects; D000498:Allyl Compounds; D010636:Phenols
chemical_list: D001713:Biphenyl Compounds; D017705:Lignans; C005498:magnolol; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols