Inhibition of Porphyromonas gingivalis hemagglutinating activity by IgY against a truncated HagA.
Porphyromonas gingivalis has been implicated as an important pathogen in the development of periodontitis. Hemagglutinins have been identified as important adhesion molecules, allowing Porphyromonas gingivalis to adhere to gingival tissue cells, and to attach and lyse erythrocytes in order to uptake Fe ions as essential nutrition. One hemagglutinin, hemagglutinin A (HagA), has been molecularly cloned via functional screening for hemagglutinating activity. We previously cloned the gene encoding the 200-kDa cell-surface antigenic protein that was reacted by sera from periodontitis patients and was identified as a truncated protein of HagA by nucleotide sequence analysis. We further subcloned the gene encoding an 122-kDa protein (122k-HagA) which is a fusion protein composed of an 80-kDa truncated HagA containing the functional motif PVQNLT and a 42-kDa maltose binding protein. Passive immunization against infectious pathogens by specific antibodies produced from hen egg yolk antibody (IgY) has been extensively developed. In the present study, to develop passive immunotherapy against periodontal disease, we purified the recombinant 122k-HagA and used this to immunize hens and produce IgY. The purified IgY reacted with the recombinant 122k-HagA and the synthetic peptide containing PVQNLT, and inhibited hemagglutinating activity of Porphyromonas gingivalis. Thus, the novel IgY may be useful in the development of a passive immunization against periodontal diseases caused by P. gingivalis infection.
Tezuka
Akihide
A
Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
Hamajima
Susumu
S
Hatta
Hajime
H
Abiko
Yoshimitsu
Y
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors* / Q000737:chemistry; D015153:Blotting, Western; D002645:Chickens; D005260:Female; D006384:Hemagglutination / Q000187:drug effects*; D007116:Immunization, Passive; D007136:Immunoglobulins / Q000276:immunology* / Q000494:pharmacology*; D037102:Lectins / Q000037:antagonists & inhibitors / Q000737:chemistry; D016966:Porphyromonas gingivalis / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000037:antagonists & inhibitors
D001426:Bacterial Proteins; C016512:IgY; D007136:Immunoglobulins; D037102:Lectins; D011993:Recombinant Fusion Proteins; C114923:hemagglutinin A, Porphyromonas gingivalis