title: Evaluation of immunodiagnostics for toxocarosis in experimental porcine cysticercosis.
abstract: We assessed whether immunodiagnostic tests for cysticercosis can cross-react with the currently available immunodiagnostic tests for Toxocara canis in an established animal model for cysticercosis infection in pigs, known host for Toxocara. We examined by TES-enzyme-linked immunosorbent test and immunoblot assay for toxocarosis and cysticercosis the baseline and final follow-up sera of 10 pigs, before and after (3 months) infection with Taenia solium. After successful cysticercosis infection, the nine evaluable pigs became seropositive to T. solium (enzyme-linked immunoelectrotransfer blot assay), but did remain seronegative for Toxocara in both assays, documenting the lack of cross-reactivity with anti-T. solium antibodies in both T. canis assays. These findings should help clinicians better interpret serology for toxocariosis and cysticercosis in endemic areas for both helminth infections.
authors.affiliation: Department of Microbiology, Universidad Peruana Cayetano Heredia, San Martin de Porres, Lima, Peru.
authors.forename: Hector H
authors.initials: HH
authors.lastname: García
authors.forename: Gabriella
authors.initials: G
authors.lastname: Cancrini
authors.forename: Filippo
authors.initials: F
authors.lastname: Bartalesi
authors.forename: Silvia
authors.initials: S
authors.lastname: Rodriguez
authors.forename: Juan A
authors.initials: JA
authors.lastname: Jimenez
authors.forename: William
authors.initials: W
authors.lastname: Roldan
authors.forename: Antonia
authors.initials: A
authors.lastname: Mantella
authors.forename: Alessandra
authors.initials: A
authors.lastname: Nicoletti
authors.forename: Alessandro
authors.initials: A
authors.lastname: Bartoloni
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology; D003429:Cross Reactions / Q000276:immunology; D003551:Cysticercosis / Q000175:diagnosis* / Q000276:immunology; D004195:Disease Models, Animal; D019353:Endemic Diseases; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D015151:Immunoblotting / Q000379:methods; D013552:Swine; D017166:Toxocara canis / Q000276:immunology*; D014120:Toxocariasis / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth