title: Proteomic analysis of cervical-vaginal fluid: identification of novel biomarkers for detection of intra-amniotic infection.
abstract: Intra-amniotic infection (IAI) is associated with preterm birth and perinatal mortality. To identify potential biomarkers, we performed a comprehensive survey of the cervical-vaginal fluid (CVF) proteome from a primate IAI model utilizing multidimensional protein identification technology (LC/LC-MS/MS) and MALDI-TOF-MS analyses. Analyses of CVF proteome identified 205 unique proteins and differential expression of 27 proteins in controls and IAI samples. Protein expression signatures and immunodetection of specific biomarkers identified can be employed for noninvasive detection of IAI.
authors.affiliation: Division of Reproductive Sciences, Oregon National Primate Research Center, Beaverton, Oregon 97006, USA. gravettm@u.washington.edu
authors.forename: Michael G
authors.initials: MG
authors.lastname: Gravett
authors.forename: Archana
authors.initials: A
authors.lastname: Thomas
authors.forename: Kimberly A
authors.initials: KA
authors.lastname: Schneider
authors.forename: Ashok P
authors.initials: AP
authors.lastname: Reddy
authors.forename: Surendra
authors.initials: S
authors.lastname: Dasari
authors.forename: Thomas
authors.initials: T
authors.lastname: Jacob
authors.forename: Xinfang
authors.initials: X
authors.lastname: Lu
authors.forename: Matthew
authors.initials: M
authors.lastname: Rodland
authors.forename: Leonardo
authors.initials: L
authors.lastname: Pereira
authors.forename: Drew W
authors.initials: DW
authors.lastname: Sadowsky
authors.forename: Charles T
authors.initials: CT
authors.lastname: Roberts
authors.forename: Miles J
authors.initials: MJ
authors.lastname: Novy
authors.forename: Srinivasa R
authors.initials: SR
authors.lastname: Nagalla
mesh_terms: D000653:Amniotic Fluid / Q000382:microbiology*; D000818:Animals; D015415:Biomarkers / Q000737:chemistry*; D001826:Body Fluids / Q000378:metabolism* / Q000382:microbiology; D002584:Cervix Uteri / Q000378:metabolism* / Q000382:microbiology; D005260:Female; D005786:Gene Expression Regulation; D007239:Infections / Q000175:diagnosis*; D008253:Macaca mulatta; D011247:Pregnancy; D011270:Pregnancy, Animal; D047928:Premature Birth / Q000517:prevention & control; D040901:Proteomics / Q000379:methods*; D014509:Ureaplasma / Q000378:metabolism; D014621:Vagina / Q000378:metabolism* / Q000382:microbiology
chemical_list: D015415:Biomarkers