The DNA glycosylase/lyase ROS1 functions in pruning DNA methylation patterns in Arabidopsis.
The Arabidopsis DNA glycosylase/lyase ROS1 participates in active DNA demethylation by a base-excision pathway. ROS1 has been shown to be required for demethylating a transgene promoter. To determine the function of ROS1 in demethylating endogenous loci, we carried out bisulfite-sequencing analysis of several transposons and other genes in the ros1 mutant. In the wild-type, although CpG sites at the majority of these loci are heavily methylated, many of the CpXpG and CpXpX sites have low levels of methylation or are not at all methylated. However, these CpXpG and CpXpX sites become heavily methylated in the ros1 mutant. Associated with this increased DNA methylation, these loci show decreased expression in the ros1 mutant. Our results suggest that active DNA demethylation is important in pruning the methylation patterns of the genome, and even the normally "silent" transposons are under dynamic control by both methylation and demethylation. This dynamic control may be important in keeping the plant epigenome plastic so that it can efficiently respond to developmental and environmental cues.
Zhu
Jianhua
J
Center for Plant Cell Biology, Institute for Integrative Genome Biology and Department of Botany and Plant Sciences, University of California, Riverside, California 92521, USA.
Kapoor
Avnish
A
Sridhar
Vaniyambadi V
VV
Agius
Fernanda
F
Zhu
Jian-Kang
JK
D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D019175:DNA Methylation; D004251:DNA Transposable Elements; D020862:GC Rich Sequence; D018745:Genome, Plant; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*
D029681:Arabidopsis Proteins; D004251:DNA Transposable Elements; D009687:Nuclear Proteins; C471271:ROS1 protein, Arabidopsis