title: ERp57 and PDI: multifunctional protein disulfide isomerases with similar domain architectures but differing substrate-partner associations.
abstract: Secretory proteins become folded and acquire stabilizing disulfide bonds in the endoplasmic reticulum (ER). Correct disulfide bond formation is a key step in ER quality control (ERQC). Proteins with incorrect disulfide bonds are recognized by the quality control machinery and are retrotranslocated into the cytosol where they are degraded by the proteasome. The mammalian ER contains 17 disulfide isomerases and at least one of them, ERp57, works in conjunction with the ER lectin-like chaperones calnexin and calreticulin. The targeting of ERp57 to calnexin-calreticulin is mediated by its noncatalytic b' domain, and analogous domains in other disulfide isomerases likely determine their substrate and partner preferences. This review discusses some explanations for the multiplicity of disulfide isomerases and highlights structural differences in the b' domains of PDI and ERp57 as an example of how noncatalytic domains define specialized roles in oxidative folding.
authors.affiliation: Biochemistry Department, McGill University, 3655 Promenade Sir William Osler, Montréal, QC H3G 1Y6, Canada. veli-pekka.maatanen@po-box.mcgill.ca
authors.forename: P
authors.initials: P
authors.lastname: Maattanen
authors.forename: G
authors.initials: G
authors.lastname: Kozlov
authors.forename: K
authors.initials: K
authors.lastname: Gehring
authors.forename: D Y
authors.initials: DY
authors.lastname: Thomas
mesh_terms: D000595:Amino Acid Sequence; D037281:Calnexin / Q000378:metabolism; D037282:Calreticulin / Q000378:metabolism; D004220:Disulfides / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019704:Protein Disulfide-Isomerases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity
chemical_list: D037282:Calreticulin; D004220:Disulfides; D037281:Calnexin; D019704:Protein Disulfide-Isomerases