Colonization capacity and serum bactericidal activity of Haemophilus parasuis thy mutants.
The bacterial thyA gene encodes the enzyme thymidylate synthase, which is essential for dTMP synthesis and, consequently, for DNA replication. In this work, a Haemophilus parasuis thyA mutant was constructed in order to analyze its colonization characteristics and its capacity to generate serum bactericidal activity in infected guinea pigs. The data showed that colonization by the H. parasuis thyA mutant was much less than that of the wild-type strain. Nevertheless, the mutant generated a strong immunogenic response in the host, as detected by measuring serum bactericidal activity.
Bigas
Anna
A
Department of Genetics and Microbiology, Autonomous University of Barcelona, Bellaterra, Spain.
Garrido
M Elena
ME
Badiola
Ignacio
I
Barbé
Jordi
J
Llagostera
Montserrat
M
D000818:Animals; D001770:Blood Bactericidal Activity; D006168:Guinea Pigs; D006192:Haemophilus Infections / Q000097:blood / Q000276:immunology* / Q000382:microbiology; D044137:Haemophilus parasuis / Q000235:genetics / Q000254:growth & development / Q000276:immunology / Q000502:physiology*; D013940:Thymidylate Synthase / Q000235:genetics / Q000378:metabolism
D013940:Thymidylate Synthase