A new test for long-term spatial memory using an operant chamber in mice.
As part of ongoing efforts to develop fully automated and standardized behavioral tasks to probe cognitive and mnemonic capabilities of mice, we have constructed a new rectangular operant chamber. The chamber contains numerous nose poke holes, distributed over three of its inner walls that are identifiable by their spatial locations. Using this apparatus, we have developed a 'spatial' memory task using a successive reversal discrimination paradigm. Mice learn to discriminate, by trial and error, the position of a single valid hole during a Presentation session wherein they obtained a maximum of 20 reinforcements or 15 min time elapsed. Following a delay interval, they were resubmitted to the same task (Test) using the same reinforced hole. Results indicated that C57BL/6 mice exhibited a significant improvement during the Test, the magnitude of the improvement (memory savings) being dependent on the length of retention intervals ranging from 5 min to 24h. In addition, discrimination performance was sensitive to scopolamine in a dose dependent manner. The simplicity in task set up and the minimal labor and space requirements make this task suitable for high throughput behavioral characterization of genetically modified mice.
Delcasso
Sebastien
S
Laboratory of Cognitive Neuroscience, CNRS UMR 5106, Avenue des Facultés 33405 Talence Cedex, France.
Jeantet
Yannick
Y
Cho
Yoon H
YH
D000704:Analysis of Variance; D000818:Animals; D035841:Behavioral Research / Q000379:methods*; D003216:Conditioning, Operant / Q000187:drug effects / Q000502:physiology*; D004193:Discrimination Learning / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D005106:Exploratory Behavior / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008568:Memory / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018727:Muscarinic Antagonists / Q000494:pharmacology; D015203:Reproducibility of Results; D012601:Scopolamine / Q000494:pharmacology; D013037:Spatial Behavior / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors
D018727:Muscarinic Antagonists; D012601:Scopolamine