title: UV spectrophotometric determination of bioflavonoids from a semisolid pharmaceutical dosage form containing Trichilia catigua Adr. Juss and Ptychopetalum olacoides Bentham standardized extract: analytical method validation and statistical procedures.
abstract: A precise, accurate, and sensitive UV spectrophotometric method was developed and validated for routine quantification of total bioflavonoids, expressed as rutin, from a topical oil-in-water pharmaceutical emulsion containing the extract of Trichilia catigua Adr. Juss and Ptychopetalum olacoides Bentham. The method was validated experimentally, and the data were treated rigorously by statistical analysis. The following analytical parameters were assessed: linearity, specificity, intra- and interrun precision measured as relative standard deviation (RSD, %), intra- and interrun accuracy (E, %), recovery (Rec., %), limit of detection (LOD, microg/mL), and limit of quantification (LOQ, microg/mL). The UV spectrophotometric method was linear (r = 0.9995) for standard rutin over the concentration range of 5.0-15.0 microg/mL with specificity for total bioflavonoids (expressed as rutin) at 361.0 nm with an absence of interferents from the complex matrix; RSD of < or = 1.79%, intrarun (E = 97.88 +/- 1.75 to 99.0 +/- 0.33%) and interrun (E = 98.38 +/- 1.12 to 100.79 +/- 1.30%) accuracy; Rec. = 98.64 +/- 0.42 to 100.74 +/- 0.41%; LOD = 0.20 microg/mL; and LOQ = 0.30 microg/mL.
authors.affiliation: University of São Paulo, School of Pharmaceutical Sciences, Department of Pharmacy, FCF-USP, 580 Prof. Lineu Prestes Ave, Bloco 13, Conjunto das Quimicas, Cidade Universitária, 05508-900, São Paulo, Brazil. andrerb@usp.br
authors.forename: André Rolim
authors.initials: AR
authors.lastname: Baby
authors.forename: Carolina P M
authors.initials: CP
authors.lastname: Maciel
authors.forename: Telma M
authors.initials: TM
authors.lastname: Kaneko
authors.forename: Maria Valéria R
authors.initials: MV
authors.lastname: Velasco
mesh_terms: D000287:Administration, Topical; D000704:Analysis of Variance; D002138:Calibration; D003627:Data Interpretation, Statistical; D005419:Flavonoids / Q000032:analysis*; D007202:Indicators and Reagents; D029582:Meliaceae / Q000737:chemistry*; D031656:Olacaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis; D012015:Reference Standards; D015203:Reproducibility of Results; D012431:Rutin / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005419:Flavonoids; D007202:Indicators and Reagents; D010936:Plant Extracts; D012431:Rutin