Ex vivo tissue discrimination by visible and near-infrared spectra with chemometrics.
The risk of infections from zoonotic pathogens of tissues and/or tissue-derived products has been increasing. One preventive approach in reducing infection risk is tissue decontamination, where selection and screening of highly infectious tissues are strictly followed. Therefore, the development of reliable analytical methods for rapid tissue discrimination is essentially important. In the present study, a procedure has been developed for intact tissue discrimination on the basis of multivariate analysis of visible and near-infrared (Vis-NIR) spectra of certain tissues such as brain, liver, kidney and testis of mice without any pretreatment. Transmittance spectra in the 600- to 1000-nm regions were subjected to a principal component analysis (PCA), and leave-out cross-validation was employed to develop multivariate models for tissue discrimination. The plot of PCA scores against Vis-NIR spectra of brains, kidneys, livers and testes from 11 mice portrayed reliable tissue discrimination. This result suggests that Vis-NIR spectroscopy combined with chemometrics analysis may provide a potentially useful approach for rapid non-destructive discrimination of tissues.
Sakudo
Akikazu
A
Department of Molecular Immunology, School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Tsenkova
Roumiana
R
Tei
Kyoko
K
Morita
Hiroyuki
H
Ikuta
Kazuyoshi
K
Onodera
Takashi
T
D000818:Animals; D001923:Brain Chemistry; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D025341:Principal Component Analysis / Q000379:methods; D013053:Spectrophotometry / Q000379:methods*; D019265:Spectroscopy, Near-Infrared / Q000379:methods*; D013737:Testis / Q000737:chemistry