[Study on bile excretion of scutellarein].
OBJECTIVE
A HPLC-ECD method was established to determine scutellarin in rat bile.


METHODS
The analytical column was Prontosil C18 (4.6 mm x 250 mm, 5 microm), and the mobile phase was consisted of methanol, 50 mmol x L(-1) phosphate buffer (adjusted by phosphoric acid to pH 2. 6), and tetrahydrofuran (40: 60: 10) , the flow rate was 1.0 mL x min(-1); the potential electrode voltage was 100 mv.


RESULTS
Concentration profile of scutellarin in rat bile was shown in this paper after oral administration of scutellarein.


CONCLUSIONS
Only scutellarin was detected in rat bile, while both of scutellarin and scutellarein were detected in rat plasma.
Che
Qing-Ming
QM
School of Pharmaceutical Sciences, Beijing University, Beijing 100083, China. cheqing-ming2003@sina.com
Chen
Ying
Y
Pan
Li-Yi
LY
He
Hong
H
D000818:Animals; D047310:Apigenin / Q000032:analysis* / Q000097:blood / Q000302:isolation & purification*; D001646:Bile / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D049919:Erigeron / Q000737:chemistry; D005965:Glucuronates / Q000032:analysis*; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
D005965:Glucuronates; C484876:scutellarin; D047310:Apigenin; C458179:scutellarein