Biodegradation of bisphenol A and related compounds by Sphingomonas sp. strain BP-7 isolated from seawater.
A bacterium capable of assimilating 2,2-bis(4-hydroxyphenyl)propane (bisphenol A), strain BP-7, was isolated from offshore seawater samples on a medium containing bisphenol A as sole source of carbon and energy, and identified as Sphingomonas sp. strain BP-7. Other strains, Pseudomonas sp. strain BP-14, Pseudomonas sp. strain BP-15, and strain no. 24A, were also isolated from bisphenol A-enrichment culture of the seawater. These strains did not degrade bisphenol A, but accelerated the degradation of bisphenol A by Sphingomonas sp. strain BP-7. A mixed culture of Sphingomonas sp. strain BP-7 and Pseudomonas sp. strain BP-14 showed complete degradation of 100 ppm bisphenol A within 7 d in SSB-YE medium, while Sphingomonas sp. strain BP-7 alone took about 40 d for complete consumption of bisphenol A accompanied by accumulation of 4-hydroxyacetophenone. On a nutritional supplementary medium, Sphingomonas sp. strain BP-7 completely degraded bisphenol A and 4-hydroxyacetophenone within 20 h. The strain degraded a variety of bisphenols, such as 1,1-bis(4-hydroxyphenyl)ethane, 2,2-bis(4-hydroxy-3-methylphenyl)propane, 2,2-bis(4-hydroxyphenyl)butane, and 1,1-bis(4-hydroxyphenyl)cyclohexane, and hydroxy aromatic compounds such as 4-hydroxyacetophenone, 4-hydroxybenzoic acid, catechol, protocatechuic acid, and hydroquinone. The strain did not degrade bis(4-hydroxyphenyl)methane, bis(4-hydroxyphenyl)sulfone, or bis(4-hydroxyphenyl)sulfide.
Sakai
Kiyofumi
K
Department of Biochemistry, Osaka Municipal Technical Research Institute, Japan. sakai@omtri.city.osaka.jp
Yamanaka
Hayato
H
Moriyoshi
Kunihiko
K
Ohmoto
Takashi
T
Ohe
Tatsuhiko
T
D001559:Benzhydryl Compounds; D003470:Culture Media; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry* / Q000145:classification / Q000378:metabolism*; D012623:Seawater / Q000382:microbiology*; D020578:Sphingomonas / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D003470:Culture Media; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds