Immunohistochemical analysis for cytokeratin 7, KIT, and PAX2: value in the differential diagnosis of chromophobe cell carcinoma.
Immunohistochemical staining for cytokeratin 7 (CK7), KIT, and PAX2 expression was performed on 91 renal neoplasms, 37 conventional (clear cell) renal cell carcinomas (CRCCs), 20 papillary RCCs (PRCCs), 11 chromophobe RCCs (ChCs), and 23 oncocytomas, with available karyotypes. All ChCs, 19 PRCCs, 2 CRCCs, and 1 oncocytoma were CK7+; all ChCs, 22 oncocytomas, 2 CRCCs, and no PRCCs expressed KIT; PAX2 was positive in 31 CRCCs, 17 PRCCs, 20 oncocytomas, and 1 ChC. The predominant expression profiles were as follows: CRCC, CK7-/KIT-/PAX2+ (26/37); PRCC, CK7+/KIT-/PAX2+ (17/20); ChC, CK7+/KIT+/PAX2- (10/11); and oncocytoma, CK7-/KIT+/PAX2+ (19/23). Cytogenetic analysis showed that the sole PAX2+ ChC had a retained chromosome 10, and all ChCs with chromosome 10 loss were PAX2-. These results identify specific staining patterns of the 4 major histologic subtypes of renal neoplasms and raise the question of a relationship between chromosome 10 loss and loss of PAX2 expression in ChC.
Memeo
Lorenzo
L
Department of Pathology Unit, Mediterranean Institute of Oncology, Catania, Italy.
Jhang
Jeffrey
J
Assaad
Adel M
AM
McKiernan
James M
JM
Murty
Vundavalli V V S
VV
Hibshoosh
Hanina
H
Tong
Guo-Xia
GX
Mansukhani
Mahesh M
MM
D018249:Adenoma, Oxyphilic / Q000737:chemistry; D002292:Carcinoma, Renal Cell / Q000737:chemistry* / Q000175:diagnosis; D020732:Cytogenetic Analysis; D003937:Diagnosis, Differential; D006801:Humans; D007150:Immunohistochemistry; D053552:Keratin-7 / Q000032:analysis*; D007680:Kidney Neoplasms / Q000737:chemistry*; D051762:PAX2 Transcription Factor / Q000032:analysis*; D019009:Proto-Oncogene Proteins c-kit / Q000032:analysis*
D053552:Keratin-7; D051762:PAX2 Transcription Factor; C497318:PAX2 protein, human; D019009:Proto-Oncogene Proteins c-kit