title: Biochemical implications of a three-dimensional model of monomeric actin bound to magnesium-chelated ATP.
abstract: Actin structure is of intense interest in biology due to its importance in cell function and motility mediated by the spatial and temporal regulation of actin monomer-filament interconversions in a wide range of developmental and disease states. Despite this interest, the structure of many functionally important actin forms has eluded high-resolution analysis. Due to the propensity of actin monomers to assemble into filaments structural analysis of Mg-bound actin monomers has proven difficult, whereas high-resolution structures of actin with a diverse array of ligands that preclude polymerization have been quite successful. In this work, we provide a high-resolution structural model of the Mg-ATP-actin monomer using a combination of computational methods and experimental footprinting data that we have previously published. The key conclusion of this study is that the structure of the nucleotide binding cleft defined by subdomains 2 and 4 is essentially closed, with specific contacts between two subdomains predicted by the data.
authors.affiliation: Case Center for Proteomics, Case Western Reserve University, 10090 Euclid Avenue, Cleveland, OH 44106, USA. keiji.takamoto@case.edu
authors.forename: Keiji
authors.initials: K
authors.lastname: Takamoto
authors.forename: J K Amisha
authors.initials: JK
authors.lastname: Kamal
authors.forename: Mark R
authors.initials: MR
authors.lastname: Chance
mesh_terms: D000199:Actins / Q000737:chemistry*; D000255:Adenosine Triphosphate / Q000737:chemistry*; D000818:Animals; D002384:Catalysis; D002417:Cattle; D002614:Chelating Agents / Q000737:chemistry; D019295:Computational Biology; D018360:Crystallography, X-Ray; D030562:Databases, Protein; D008274:Magnesium / Q000737:chemistry*; D008958:Models, Molecular; D011487:Protein Conformation; D023302:Protein Footprinting; D017434:Protein Structure, Tertiary; D051381:Rats
chemical_list: D000199:Actins; D002614:Chelating Agents; D000255:Adenosine Triphosphate; D008274:Magnesium