title: [Determination of ginsenoside rd in human plasma by LC/MS/MS].
abstract: OBJECTIVE
  To develop a HPLC/MS/MS method for the determination of ginsenoside Rd in human plasma.
  
  
  METHODS
  Plasma samples were pretreated by solid phase extraction (SPE). Ginsenoside Rd (m/z 964.6-->m/z 767.5) and gentiopicrin (m/z 374.1-->195.1) was detected by the positive electrospray ionization (ES+I)-MS method under multiple reaction monitoring (MRM) mode.
  
  
  RESULTS
  The calibration curve of ginsenoside Rd in plasma was linear over the range of 3.00-5000.00 ng/ml. The limit of quantitation was 3.00 ng/ml. The relative recovery was 81.01-83.39%. The within-day and between-day RSDs were less than 15%. CONCLUSION This method is accurate, sensitive, and specific. It is suitable for the measurement of plasma ginsenoside Rd concentration.
authors.affiliation: Department of Clinical Pharmacology, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510120, China.
authors.forename: Yuan-hui
authors.initials: YH
authors.lastname: Deng
authors.forename: Yi
authors.initials: Y
authors.lastname: Feng
authors.forename: Xing
authors.initials: X
authors.lastname: Zeng
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
authors.forename: Yi-ming
authors.initials: YM
authors.lastname: Liu
authors.forename: Yu
authors.initials: Y
authors.lastname: Huang
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Dan
authors.initials: D
authors.lastname: Zhou
authors.forename: Wei-xiong
authors.initials: WX
authors.lastname: Liang
mesh_terms: D019540:Area Under Curve; D001682:Biological Availability; D004355:Drug Stability; D036145:Ginsenosides / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D007275:Injections, Intravenous; D010946:Plants, Medicinal / Q000737:chemistry; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D036145:Ginsenosides