title: [Role of AcSDKP on collagen synthesis and degradation in cultured rat cardiac fibroblast].
abstract: OBJECTIVE
  To investigate the role of AcSDKP on collagen synthesis and degradation in cultured rat cardiac fibroblasts.
  
  
  METHODS
  Neonatal rat cardiac fibroblasts were isolated and stimulated by PDGF. The cell proliferation was observed by (3)H-TdR incorporation assay. The synthesis of collagen was measured by (3)H-proline incorporation assay. The expression of type I and type III collagen and MMP-1 protein were measured by Western blot. The MMP-2 and MMP-9 activity was evaluated with zymography assay.
  
  
  RESULTS
  PDGF stimulated cardiac fibroblasts proliferation with increased collagen synthesis and type I and type III collagen protein expressions as well as MMP-2 and MMP-9 activities and MMP-1 expression. AcSDKP inhibited cardiac fibroblasts proliferation induced by PDGF and reduced collagen synthesis and type I and type III collagen protein expression. AcSDKP also further up-regulated MMP-2 and MMP-9 activities and MMP-1 expression in cardiac fibroblasts induced by PDGF.
  
  
  CONCLUSIONS
  AcSDKP inhibited proliferation and collagen synthesis and up-regulated matrix metalloproteinases activity or expression induced by PDGF, which was possibly related with the effect of AcSDKP anti-fibrosis.
authors.affiliation: Experimental and Research Center, North China Coal Medical College, Tangshan 063000, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Yang
authors.forename: Xi-ling
authors.initials: XL
authors.lastname: Zhu
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Wang
authors.forename: Xu-dong
authors.initials: XD
authors.lastname: Song
authors.forename: Rui-min
authors.initials: RM
authors.lastname: Wang
authors.forename: Zhi-guo
authors.initials: ZG
authors.lastname: Li
authors.forename: Ling
authors.initials: L
authors.lastname: Luo
authors.forename: Wan-mi
authors.initials: WM
authors.lastname: Hu
authors.forename: Wen-dong
authors.initials: WD
authors.lastname: Ma
authors.forename: Xin
authors.initials: X
authors.lastname: Pei
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Zhang
authors.forename: Qi-jia
authors.initials: QJ
authors.lastname: Li
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism*; D009842:Oligopeptides / Q000502:physiology*; D010982:Platelet-Derived Growth Factor / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
chemical_list: D009842:Oligopeptides; D010982:Platelet-Derived Growth Factor; D003094:Collagen; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; C058504:goralatide