Burkholderia soli sp. nov., isolated from soil cultivated with Korean ginseng.
A polyphasic study was carried out to clarify the taxonomic position of a Gram-negative bacterium isolated from soil cultivated with Korean ginseng in the Eumseong region of Korea. The novel strain, GP25-8(T), grew optimally at pH 6-7, 28 degrees C and 0-1 % NaCl (w/v). The major fatty acids were C(18 : 1)omega7c, summed feature 3 (C(16 : 1)omega7c/C(15 : 0) iso 2-OH) and C(16 : 0) (together representing 71.2 % of the total). The 16S rRNA gene sequence similarities between strain GP25-8(T) and members of the genus Burkholderia ranged from 94.7 to 97.4 %, indicating that this novel strain was phylogenetically related to members of that genus. The novel strain showed the highest sequence similarities to Burkholderia caryophylli ATCC 25418(T) (97.4 %) and Burkholderia phenazinium LMG 2247(T) (97.2 %); the levels of DNA-DNA hybridization with these strains were 28 and 12 %, respectively. These results support the conclusion that strain GP25-8(T) represents a novel species within the genus Burkholderia, for which the name Burkholderia soli sp. nov. is proposed. The type strain is GP25-8(T) (=KACC 11589(T)=DSM 18235(T)).
Yoo
Seung-Hee
SH
Korean Agricultural Culture Collection, Microbial Genetics Division, National Institute of Agricultural Biotechnology, National Institute of Agricultural Science and Technology, Rural Development Administration, Suwon 441-707, Korea.
Kim
Byung-Yong
BY
Korean Agricultural Culture Collection, Microbial Genetics Division, National Institute of Agricultural Biotechnology, National Institute of Agricultural Science and Technology, Rural Development Administration, Suwon 441-707, Korea.
Weon
Hang-Yeon
HY
Applied Microbiology Division, National Institute of Agricultural Science and Technology, Rural Development Administration, Suwon 441-707, Korea.
Kwon
Soon-Wo
SW
Korean Agricultural Culture Collection, Microbial Genetics Division, National Institute of Agricultural Biotechnology, National Institute of Agricultural Science and Technology, Rural Development Administration, Suwon 441-707, Korea.
Go
Seung-Joo
SJ
Korean Agricultural Culture Collection, Microbial Genetics Division, National Institute of Agricultural Biotechnology, National Institute of Agricultural Science and Technology, Rural Development Administration, Suwon 441-707, Korea.
Stackebrandt
Erko
E
Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, D-38124 Braunschweig, Germany.
D000383:Agriculture; D015373:Bacterial Typing Techniques; D019117:Burkholderia / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D007723:Korea; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D005894:Panax / Q000254:growth & development*; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S