Comparison of histone modifications in in vivo and in vitro fertilization mouse embryos.
Histone modifications are thought to play important roles in various cellular functions. In this article, the distribution patterns of acetylation on histone H4, methylation on histone H3 lysine 9, and phosphorylation on histone H3 serine 10 were examined in in vivo and in vitro fertilization (IVF) preimplantation mouse embryos by using indirect immunofluorescence and scanning confocal microscopy. We desired to know whether the IVF, which has been widely used as a routine assisted reproductive technology in animal and human, was safe at the epigenetic level. As results, we found that there was no difference in these histone modification patterns in in vivo and IVF mouse embryos from zygote to blastocyst stage. Moreover, these histone modifications had different distributions at all examined stages, but they were consistent with the mouse embryo developmental stages.
Huang
Jun-Cheng
JC
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China.
Lei
Zi-Li
ZL
Shi
Li-Hong
LH
Miao
Yi-Liang
YL
Yang
Ji-Wen
JW
Ouyang
Ying-Chun
YC
Sun
Qing-Yuan
QY
Chen
Da-Yuan
DY
D000107:Acetylation; D000818:Animals; D019175:DNA Methylation; D004622:Embryo, Mammalian / Q000378:metabolism*; D044127:Epigenesis, Genetic / Q000502:physiology; D005260:Female; D005306:Fertilization / Q000502:physiology*; D005307:Fertilization in Vitro; D006657:Histones / Q000378:metabolism*; D051379:Mice; D010766:Phosphorylation; D011247:Pregnancy; D014018:Tissue Distribution
D006657:Histones