title: Induction of apoptosis and transient increase of phosphorylated MAPKs by diallyl disulfide treatment in human nasopharyngeal carcinoma CNE2 cells.
abstract: This study was undertaken to elucidate the effect of diallyl disulfide (DADS), an oil-soluble organosulfur compound found in garlic, in suppressing human nasopharyngeal carcinoma cells. A potent increase (of at least 9-fold) in apoptotic cells has accompanied 1) a decrease in cell viability, 2) a increase of the fraction of S-phase cells by up to 63.8%, and 3) a transient increase of the phospho-p38 and phospho-p42/44 (phosphorylated p38 MAPK and phosphorylated p42/44 MAPK) in a time- and concentration-dependent manner. These results indicate that DADS can induce apoptosis in human nasopharyngeal carcinoma cells via, at least partly, S-phase block of the cell cycle, related to a rise in MAPK phosphorylation.
authors.affiliation: Research Institute for Molecular Pharmacology and Therapeutics, Central South University, Changsha, Hunan 410083, China.
authors.forename: Yi Wei
authors.initials: YW
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Wen
authors.forename: Jian Bo
authors.initials: JB
authors.lastname: Xiao
authors.forename: Simon G
authors.initials: SG
authors.lastname: Talbot
authors.forename: Gloria C
authors.initials: GC
authors.lastname: Li
authors.forename: Ming
authors.initials: M
authors.lastname: Xu
mesh_terms: D000498:Allyl Compounds / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002277:Carcinoma / Q000188:drug therapy*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004220:Disulfides / Q000494:pharmacology*; D005737:Garlic / Q000737:chemistry; D006801:Humans; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D009303:Nasopharyngeal Neoplasms / Q000188:drug therapy*; D010766:Phosphorylation; D016196:S Phase / Q000187:drug effects; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D000498:Allyl Compounds; D004220:Disulfides; C028009:diallyl disulfide; D019950:Mitogen-Activated Protein Kinase 1; D048051:p38 Mitogen-Activated Protein Kinases