Antioxidant properties of dihydroherbimycin A from a newly isolated Streptomyces sp.
During antioxidant screening using 1,1-diphenyl-picrylhydrazyl (DPPH) and a lipid peroxidation assay, a streptomycete strain was found to produce herbimycin A and dihydroherbimycin A as antioxidants in the culture filtrate. These molecules were identified by using spectral analyses, including infrared, ultraviolet, mass spectrum, and nuclear magnetic resonance assays. In the DPPH radical-scavenging assay, dihydroherbimycin A exhibited more potent antioxidant activity (IC(50), 1.3 microM) than alpha-tocopherol (IC(50), 2.7 microM) that was used as a reference compound. In the lipid peroxidation assay, both herbimycin A and dihydroherbimycin A demonstrated antioxidant activities of 61% and 72%, respectively, at 100 microg/ml, while alpha-tocopherol exhibited an activity of 93% at the same concentration. Therefore, dihydroherbimycin A might have the potential to be developed into a new therapeutic agent.
Chang
Hung Bae
HB
Bio Polytechnic College, Chaewoon-ri, Ganggyeong-eub, Nonsan-Si, Korea.
Kim
Jae-Heon
JH
D000975:Antioxidants / Q000737:chemistry*; D016227:Benzoquinones / Q000737:chemistry* / Q000378:metabolism*; D047029:Lactams, Macrocyclic / Q000737:chemistry* / Q000378:metabolism*; D015227:Lipid Peroxidation; D008055:Lipids / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry*; D017828:Rifabutin / Q000031:analogs & derivatives; D013045:Species Specificity; D013302:Streptomyces / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism*
D000975:Antioxidants; D016227:Benzoquinones; D047029:Lactams, Macrocyclic; D008055:Lipids; D017828:Rifabutin; C020754:herbimycin; D010100:Oxygen