Detection of bovine meat and bone meal in animal feed at a level of 0.1%.
For the control of the transmission of bovine spongiform encephalopathy in cattle via feedstuff, a real-time polymerase chain reaction assay was developed with ruminant-specific Bov-B SINE primers, SYBR Green fluorescence detection, and melting curve analysis. In formulated cattle and chicken feed samples spiked with pure bovine and sheep meat and bone meal heated at 133 degrees C for 20 min, a contamination level of 0.1% was detected.
Aarts
Henk J M
HJ
RIKILT-Institute of Food Safety, Wageningen UR, Bornsesteeg 45, 6708 PD Wageningen, The Netherlands. henk.aarts@wur.nl
Bouw
El M
el M
Buntjer
Jaap B
JB
Lenstra
Johannes A
JA
Van Raamsdonk
Leo W D
LW
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D001842:Bone and Bones / Q000737:chemistry*; D002417:Cattle; D002645:Chickens; D004247:DNA / Q000737:chemistry; D017743:Equipment Reuse; D008460:Meat / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012756:Sheep
D004247:DNA