title: Analysis of the products from enzymatic scouring of cotton.
abstract: This article discusses the analysis of the hydrolysis products from one-step scouring of cotton using pectinase and two-step scouring of cotton using lipase then cellulase, protease then cellulase, or lipase/protease then cellulase, to improve water absorbency of cotton. UV spectrophotometric analysis indicated that the pectinase scouring process produced approximately 18-fold higher amounts of reducing sugars and galacturonic acid than any of the two-step scouring processes. The production rate of reducing sugars and galacturonic acid from most of the scouring processes showed a decrease with an increase in time. HPLC analysis revealed that the lipase/protease/cellulase scouring processes produced approximately 5-fold higher amounts of 17 amino acids than the pectinase scouring process. GC analysis for 18 fatty acids (C(8)-C(24)) revealed that three major fatty acids, palmitic acid, stearic acid, and behenic acid, were found on both the scoured and the unscoured fabrics. Scoured fabrics were tested for content of proteins, extractable components, waxes, and anionic components including pectins, and some differences among the fabric scoured with different enzyme combinations were found.
authors.affiliation: Department of Materials Science, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Porntip
authors.initials: P
authors.lastname: Sae-be
authors.forename: Usa
authors.initials: U
authors.lastname: Sangwatanaroj
authors.forename: Hunsa
authors.initials: H
authors.lastname: Punnapayak
mesh_terms: D002480:Cellulase / Q000378:metabolism; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D032561:Cotton Fiber; D005227:Fatty Acids / Q000378:metabolism; D003368:Gossypium / Q000737:chemistry / Q000378:metabolism*; D006603:Hexuronic Acids / Q000378:metabolism; D006868:Hydrolysis; D008049:Lipase / Q000378:metabolism; D019308:Palmitic Acid / Q000378:metabolism; D011096:Polygalacturonase / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013229:Stearic Acids / Q000378:metabolism; D013784:Textiles / Q000032:analysis / Q000592:standards
chemical_list: D005227:Fatty Acids; D006603:Hexuronic Acids; D013229:Stearic Acids; D019308:Palmitic Acid; C031183:stearic acid; C007819:galacturonic acid; D008049:Lipase; D011096:Polygalacturonase; D002480:Cellulase; C007547:behenic acid