title: A neutralizable epitope is induced on HGF upon its interaction with its receptor cMet.
abstract: A new conformational neutralizable epitope is created on heptocyte growth factor (HGF), when it interacts with its receptor, cMet. By immunizing rabbits with HGF-cMet complex, we successfully generated a monoclonal antibody (SFN68) that inhibits HGF-cMet interaction, and blocks the biological function mediated by HGF. To define the epitope, we screened out an epitope-mimicking peptide, KSLSRHDHIHHH, from a phage display of combinatorial peptide library. In molecular mimicry this peptide bound to cMet and inhibited HGF-cMet interaction. No humoral response was induced to this epitope-mimicking peptide when immunization was done with HGF alone.
authors.affiliation: Cancer Research Institute, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-gu, Seoul, Republic of Korea.
authors.forename: Kisu
authors.initials: K
authors.lastname: Kim
authors.forename: Youngmi
authors.initials: Y
authors.lastname: Hur
authors.forename: En-Kyung
authors.initials: EK
authors.lastname: Ryu
authors.forename: Jung-Hyo
authors.initials: JH
authors.lastname: Rhim
authors.forename: Cha Yong
authors.initials: CY
authors.lastname: Choi
authors.forename: Cheol-Min
authors.initials: CM
authors.lastname: Baek
authors.forename: Jae-Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Junho
authors.initials: J
authors.lastname: Chung
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology*; D018604:Epitope Mapping; D000939:Epitopes / Q000276:immunology*; D017228:Hepatocyte Growth Factor / Q000276:immunology*; D009500:Neutralization Tests; D019151:Peptide Library; D025941:Protein Interaction Mapping; D019859:Proto-Oncogene Proteins c-met / Q000276:immunology*; D011817:Rabbits
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D019151:Peptide Library; D017228:Hepatocyte Growth Factor; D019859:Proto-Oncogene Proteins c-met