title: [The influence of Solanum lyratum Thunb extract on apoptosis and the expression of fas/fasL genes in Hela cells].
abstract: OBJECTIVE
  To explore the effects of Solanum lyratum Thunb (SL) extract on the apoptosis and the expression of fas and fasL genes in Hela cells.
  
  
  METHODS
  The proliferation inhibitory rate was evaluated by MTF assay. Induction of cell apoptosis rate was determined by flow cytometry. The expression of Fas protein was detected by two-step immunhistochemical staining. The expression of fas and fasL mRNA was detected by semi-quantitive RT-PCR.
  
  
  RESULTS
  SL extract displayed strong proliferation inhibitory effect in a dose-and-time-dependent manner against Hela cell. The rate of apoptosis was increased obviously. The expression of fas mRNA and protein was increased significantly, and fasL mRNA was decreased markedly.
  
  
  CONCLUSIONS
  SL can induce apoptosis by up-regulating expression of fas and fasL genes, and inhibit the development of Hela cells.
authors.affiliation: Department of Biochemistry and Molecular Biology, Youjiang Medical College for Nationalities, Baise 533000, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Wei
authors.forename: Chao-Gan
authors.initials: CG
authors.lastname: Li
authors.forename: Song
authors.initials: S
authors.lastname: Nong
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Zhu
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Huang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D053222:Fas Ligand Protein / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis; D019657:Solanaceae / Q000737:chemistry*; D019014:fas Receptor / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D053222:Fas Ligand Protein; D012333:RNA, Messenger; D019014:fas Receptor