title: [Porcine MSC-derived CLC induced by rabbit's cardiomyocytes extract did not induce immune reaction in vivo].
abstract: OBJECTIVE
  To compare the in vivo immune reaction of transplanting porcine MSC-derived CLC with rabbit cardiomyocytes extracts induced differentiation or in vitro cultured porcine MSC.
  
  
  METHODS
  After injecting the MSC-derived CLC or MSC to the original porcines, the number of CD4+, CD8+ T cells were determined by flow cytometry. The serum concentrations of IL-2, IL-4 were measured by ELISA, and the porcine spleen lymphocyte CTL cytotoxicity was determined by Cell Counting Kit-8 Assay.
  
  
  RESULTS
  The numbers of CD4+ and CD8+ T cells, the serum concentrations of IL-2, IL-4 and spleen lymphocyte CTL cytotoxicity were all similar in porcines received MSC-derived CLC induced by rabbit's CMs extract or MSC transplantation.
  
  
  CONCLUSIONS
  The porcine MSC-derived CLC induced by rabbit's CMs extract did not induce extra immune reaction when injected back to the original porcine.
authors.affiliation: Department of Cardiology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Xin-Xin
authors.initials: XX
authors.lastname: Cao
authors.forename: Yu-Hua
authors.initials: YH
authors.lastname: Dai
authors.forename: Shu-Yang
authors.initials: SY
authors.lastname: Zhang
authors.forename: Lian-Feng
authors.initials: LF
authors.lastname: Chen
authors.forename: Jin-Zhi
authors.initials: JZ
authors.lastname: Lai
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Yan
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001854:Bone Marrow Cells / Q000276:immunology*; D016026:Bone Marrow Transplantation / Q000276:immunology*; D015704:CD4 Antigens / Q000276:immunology; D016516:CD4-CD8 Ratio; D016827:CD8 Antigens / Q000276:immunology; D005260:Female; D008297:Male; D032383:Myocytes, Cardiac / Q000166:cytology*; D011817:Rabbits; D013552:Swine; D013556:Swine, Miniature
chemical_list: D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D016827:CD8 Antigens