Galactose oxidase immobilized on silica in an analytical determination of galactose-containing carbohydrates.
Galactose oxidase from Fusarium graminearum IMV-1060 adsorbed on, and covalently bound to, silica carriers has been used for analytical determinations of D-galactose and galactose-containing sugars. Using a flowing oxygen electrode of the Clark-type, sensor system for enzymatic analysis of water solutions of galactose-containing carbohydrates was made. Measurements were taken both in the pulse and continuous modes of a substrate flowing through a column with an immobilized biocatalyst. The linear measurement ranges for galactose-containing carbohydrates concentrations were determined.
Kondakova
Lyudmila
L
Institute of Surface Chemistry of National Academy of Sciences of Ukraine, Kyiv.
Yanishpolskii
Victor
V
Tertykh
Valentin
V
Buglova
Tat'yana
T
D000327:Adsorption; D002241:Carbohydrates / Q000032:analysis*; D003611:Dairy Products / Q000032:analysis; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005670:Fusarium / Q000201:enzymology; D005690:Galactose / Q000032:analysis*; D005692:Galactose Oxidase / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007223:Infant; D007225:Infant Food / Q000032:analysis; D007785:Lactose / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry; D012822:Silicon Dioxide
D002241:Carbohydrates; D004800:Enzymes, Immobilized; D012822:Silicon Dioxide; D005692:Galactose Oxidase; D007785:Lactose; D010100:Oxygen; D005690:Galactose