Structural basis for cyclophellitol inhibition of a beta-glucosidase.
The structural basis for beta-glucosidase inhibition by cyclophellitol is demonstrated using X-ray crystallography, enzyme kinetics and mass spectrometry.
Gloster
Tracey M
TM
York Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York, UK YO10 5YW.
Madsen
Robert
R
Davies
Gideon J
GJ
D018360:Crystallography, X-Ray; D003511:Cyclohexanols / Q000737:chemistry / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D004795:Enzyme Stability; D006868:Hydrolysis; D007700:Kinetics; D013058:Mass Spectrometry; D001617:beta-Glucosidase / Q000037:antagonists & inhibitors* / Q000378:metabolism
D003511:Cyclohexanols; D004791:Enzyme Inhibitors; C063285:cyclophellitol; D001617:beta-Glucosidase