title: Biological imaging of HEK293 cells expressing PLCgamma1 using surface-enhanced Raman microscopy.
abstract: Surface-enhanced Raman scattering (SERS) imaging has been used for the targeting and imaging of specific cancer markers in live cells. For this purpose, Au/Ag core-shell nanoparticles, conjugated with monoclonal antibodies, were prepared. The procedures to label live cells with those bimetallic nanoprobes have been developed and used for highly sensitive SERS imaging of live cells. In the present study, live HEK293 cells expressing PLCgamma1 have been used as the optical imaging target. Our results demonstrate the potential feasibility of SERS imaging technology for the highly sensitive imaging of cancer biomarkers in live cells.
authors.affiliation: Department of Applied Chemistry, Hanyang University, Ansan 426-791, South Korea.
authors.forename: Sangyeop
authors.initials: S
authors.lastname: Lee
authors.forename: Sungyong
authors.initials: S
authors.lastname: Kim
authors.forename: Jaebum
authors.initials: J
authors.lastname: Choo
authors.forename: Soon Young
authors.initials: SY
authors.lastname: Shin
authors.forename: Young Han
authors.initials: YH
authors.lastname: Lee
authors.forename: Ha Young
authors.initials: HY
authors.lastname: Choi
authors.forename: Seunghan
authors.initials: S
authors.lastname: Ha
authors.forename: Kyungho
authors.initials: K
authors.lastname: Kang
authors.forename: Chil Hwan
authors.initials: CH
authors.lastname: Oh
mesh_terms: D000911:Antibodies, Monoclonal; D002460:Cell Line; D003584:Cytological Techniques; D003952:Diagnostic Imaging / Q000379:methods*; D006046:Gold; D006801:Humans; D053768:Metal Nanoparticles; D009369:Neoplasms / Q000473:pathology*; D051966:Phospholipase C gamma / Q000032:analysis*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D000911:Antibodies, Monoclonal; D006046:Gold; D051966:Phospholipase C gamma