Immunohistochemical detection of interleukin-12 and interferon-gamma in pigs experimentally infected with Mycoplasma hyopneumoniae.
The expression of interleukin-12 (IL-12) and interferon-gamma (IFN-gamma) was examined immunohistochemically in the lungs of pigs aged 21 days infected experimentally with Mycoplasma hyopneumoniae (Mh). Ten pigs were inoculated intranasally with Mh and killed in pairs weekly from 7 to 35 days post-infection (dpi). Immunolabelling for IL-12 and IFN-gamma was usually associated with inflammation, particularly in macrophages and lymphocytes in the thickened alveolar septa and in the hyperplastic bronchus-associated lymphoid tissue (BALT). Cells positive for both cytokines were detected at 7 dpi, their numbers increasing at 14 and 21 dpi, and slightly decreasing thereafter. The results suggest that IL-12 and IFN-gamma play a role in pulmonary defence mechanisms against Mh infection.
Rodríguez
F
F
Department of Comparative Pathology, Veterinary Faculty, University of Las Palmas de Gran Canaria, Trasmontaña s/n, 35416 Arucas, Gran Canaria, Spain. frodriguez@dmor.ulpgc.es
Quesada
O
O
Poveda
J B
JB
Fernández
A
A
Lorenzo
H
H
D000818:Animals; D004195:Disease Models, Animal; D007150:Immunohistochemistry / Q000662:veterinary*; D007371:Interferon-gamma / Q000032:analysis*; D018664:Interleukin-12 / Q000032:analysis*; D008168:Lung / Q000276:immunology / Q000378:metabolism; D045705:Mycoplasma hyopneumoniae / Q000276:immunology*; D045729:Pneumonia of Swine, Mycoplasmal / Q000139:chemically induced / Q000175:diagnosis* / Q000276:immunology / Q000378:metabolism; D013552:Swine
D018664:Interleukin-12; D007371:Interferon-gamma