Effects of arachidonate metabolism inhibitors on basal and human chorionic gonadotropin-stimulated progesterone secretion by rat corpus luteum cells in vitro.
Arachidonic acid (AA) and its metabolites mediate many physiological processes including reproduction and endocrinology. The current study investigated effects of several inhibitors of AA cascade on steroidogenesis by rat corpus luteum cells in vitro. Dispersed luteal cells prepared from rat corpus luteum on day 6 of pseudopregnancy secreted progesterone (P4) in time-dependent and human chorinonic gonadotropin (hCG)-dependent fashion. Arachidonyl trifluoromethyl ketone, a preferential inhibitor of the type IVA phospholipase A(2) (PLA(2)-IVA), stimulated basal P4 secretion and had no influence on hCG-stimulated steroidogenesis. A novel and more specific inhibitor pyrrophenone inhibited hCG-induced P4 secretion. A cyclooxygenase inhibitor indomethacin did not affect basal secretion but inhibited hCG-stimulated secretion. Nordihydroguaiaretic acid tended to decrease basal P4 secretion and completely inhibited hCG-stimulated secretion. Obtained results suggest that AA metabolic cascade, which is triggered at least in part by PLA(2)-IVA activity, is potentially implicated in hCG-stimulated P4 secretory response in the rat corpus luteum.
Kurusu
Shiro
S
Laboratory of Physiology, Kitasato University School of Veterinary Medicine and Animal Sciences, Towada, Aomori 034-8628, Japan. kurusu@vmas.kitasato-u.ac.jp
Ohkawa
Mina
M
Kawaminami
Mitsumori
M
D000818:Animals; D016718:Arachidonic Acid / Q000037:antagonists & inhibitors*; D001095:Arachidonic Acids / Q000494:pharmacology; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D008184:Luteal Cells / Q000187:drug effects* / Q000378:metabolism*; D009637:Masoprocol / Q000494:pharmacology; D011374:Progesterone / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D001095:Arachidonic Acids; D006063:Chorionic Gonadotropin; D004791:Enzyme Inhibitors; C081565:arachidonyltrifluoromethane; D016718:Arachidonic Acid; D011374:Progesterone; D009637:Masoprocol; D007213:Indomethacin