title: Human muscle fatigue: the significance of muscle fibre type variability studied using a micro-dissection approach.
abstract: During human locomotion the ability to generate and sustain mechanical power output is dependent on the organised variability in contractile and metabolic properties of the muscle fibres that comprise the active muscles. In studies of human exercise we have used a micro-dissection technique to obtain fragments of single muscle fibres from needle biopsies before and after exercise. Each fibre fragment is divided into two parts. One part is used to characterize the fibre type in respect of the heavy chain myosin isoform expressed. The other part of the fragment is analysed for high energy phosphate concentrations. Fibres are classified on the basis of expressing either type I, type IIA, or type IIX myosin heavy chain isoforms. It should be noted however that in the type II population many fibres co-express both IIA and the IIX isoforms and we therefore characterize these fibres on the basis of the degree of co-expression. We have used this technique to examine the time course of high energy phosphate concentration and fatigue in different fibre populations during exercise. The progressive reduction of power during maximal sprint efforts may be interpreted as the cumulative effect of metabolic depletion in successive fibre type populations from IIX to IIXa to IIAx to IIA to I. One important application of the micro-dissection technique is that PCr content may also be used as a very sensitive metabolic marker for fibre type recruitment during very short duration concentric, isometric and eccentric exercise.
authors.affiliation: Institute for Biophysical and Clinical Research into Human Movement, Manchester Metropolitan University, Manchester, UK. a.j.sargeant@mmu.ac.uk
authors.forename: A J
authors.initials: AJ
authors.lastname: Sargeant
authors.forename: A
authors.initials: A
authors.lastname: de Haan
mesh_terms: D001642:Bicycling / Q000502:physiology; D001707:Biopsy, Needle; D004734:Energy Metabolism / Q000502:physiology; D015444:Exercise / Q000502:physiology; D005080:Exercise Test; D006801:Humans; D042282:Microdissection; D018763:Muscle Fatigue / Q000502:physiology*; D018485:Muscle Fibers, Skeletal / Q000502:physiology*; D018995:Myosin Heavy Chains / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*; D020033:Protein Isoforms
chemical_list: D010710:Phosphates; D020033:Protein Isoforms; D018995:Myosin Heavy Chains