Serum and urinary cytokine homeostasis and renal tubular function in children with type 1 diabetes mellitus.
OBJECTIVE
To explore the relationships between tumor necrosis factor-alpha (TNFalpha), interleukin-6 (IL-6) and urinary N-acetyl-beta-D-glucosaminidase (NAG) and the function of renal proximal tubules in children with type 1 diabetes mellitus (DM1).


METHODS
Fifty-six children with DM1 and 35 healthy controls were analyzed. We measured NAG (A and B isoforms) in urine as well as serum TNFalpha and urinary IL-6.


RESULTS
The children with DM1 with microalbuminuria (group A) had significantly higher urinary IL-6 and serum TNFa than the children without microalbuminuria (group B). The diabetic patients with no sign of nephropathy showed significantly higher TNFalpha and NAG and its A and B isoforms in urine compared to the healthy group. Additionally, groups A and B both showed a positive significant correlation between serum TNFalpha and urinary isoform B.


CONCLUSIONS
From our pilot results it appears that TNFalpha might be a sensitive marker of damage to the renal proximal tubules occurring prior to microalbuminuria. Conversely, the increase in NAG and its isoform B activity in patients with no clinical sign of diabetic nephropathy may indicate the onset of microalbuminuria.
Myśliwiec
Małgorzata
M
Institute of Pediatrics, Hematology, Oncology and Endocrinology, Medical University of Gdańsk, Debinki 7, 80-211 Gdańsk, Poland. mysliwiec@amg.gda.pl
Balcerska
Anna
A
Zorena
Katarzyna
K
Myśliwska
Jolanta
J
Lipska
Beata S
BS
Wiśniewski
Piotr
P
Myśliwski
Andrzej
A
D000118:Acetylglucosaminidase / Q000652:urine*; D000293:Adolescent; D000419:Albuminuria / Q000473:pathology / Q000652:urine*; D015415:Biomarkers / Q000097:blood / Q000652:urine; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000473:pathology / Q000652:urine*; D003928:Diabetic Nephropathies / Q000097:blood* / Q000652:urine*; D005260:Female; D006706:Homeostasis; D006801:Humans; D015850:Interleukin-6 / Q000652:urine*; D007527:Isoenzymes / Q000652:urine; D008297:Male; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*
D015415:Biomarkers; D015850:Interleukin-6; D007527:Isoenzymes; D014409:Tumor Necrosis Factor-alpha; D000118:Acetylglucosaminidase