title: An outbreak of teat papillomatosis in cattle caused by bovine papilloma virus (BPV) type 6 and unclassified BPVs.
abstract: Out of 700 heifers at a local farm in Hokkaido, the Northern island of Japan, 560 (80%) were found to have benign teat tumors. All of the analyzed tumors were macroscopically of the flat-and-round type, and no other types such as rice-grain or frond epithelial type were found. The lesions were characterized by epithelial hyperplasia, acanthosis and hyperkeratosis. Unlike in typical fibropapilloma, fibroplasia of the underlying dermis was not observed. Bovine papilloma virus (BPV) capsid antigen and virus particles were found in basophilic intranuclear inclusions of the stratum granulosum of the epidermis by immunohistochemistry and electron microscopy, respectively. BPV-specific DNA was also detected in the lesions. By means of the polymerase chain reaction (PCR) and DNA sequencing of the PCR products, the viruses causing this outbreak were identified mainly as BPV-6 (64%), partly as unclassified BPVs (14%) and their co-infections (21%). Our findings suggest that this outbreak of benign teat tumors was associated with several BPV types.
authors.affiliation: Hokkaido Research Station, National Institute of Animal Health, 4 Hitsujigaoka, Toyohira, Sapporo 062-0045, Japan.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Maeda
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Shibahara
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Wada
authors.forename: Koichi
authors.initials: K
authors.lastname: Kadota
authors.forename: Toru
authors.initials: T
authors.lastname: Kanno
authors.forename: Ikuo
authors.initials: I
authors.lastname: Uchida
authors.forename: Shinichi
authors.initials: S
authors.lastname: Hatama
mesh_terms: D000818:Animals; D001483:Base Sequence; D001941:Breast Diseases / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000821:virology*; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D004196:Disease Outbreaks / Q000662:veterinary*; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D007564:Japan / Q000453:epidemiology; D008321:Mammary Glands, Animal / Q000648:ultrastructure / Q000821:virology*; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment
chemical_list: D004279:DNA, Viral