Novel aerobic perchloroethylene degradation by the white-rot fungus Trametes versicolor.
Perchloroethylene (PCE) is one of the most important groundwater pollutants around the world. It is a suspected carcinogen and is believed to be recalcitrant to microbial degradation. We report here, for the first time, aerobic degradation of PCE by the white rot fungus, Trametes versicolor, to less hazardous products. Aerobic degradation rate of PCE was 0.20 and 0.28 nmol h(-1) mg(-1) dry weight of fungal biomass. Trichloroacetic acid (TCA) was identified as the main intermediate using [2-13C]-PCE as the substrate. Chloride released and TCA produced were stoichiometric with PCE degradation. Our studies using 1 -aminobenzotriazole (ABT), an inhibitor of cytochrome P-450, suggested that a cytochrome P-450 system may be involved in PCE degradation by T. versicolor. These results are of particular interest because TCA production from PCE has not been reported to date in bacteria or fungi.
Marco-Urrea
Ernest
E
Departament d'Enginyeria Química (EQ) and Institut de CiMncia i Tecnologia Ambiental (ICTA), Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
Gabarrell
Xavier
X
Sarrà
Montserrat
M
Caminal
Gloria
G
Vicent
Teresa
T
Reddy
C Adinarayana
CA
D000332:Aerobiosis; D001487:Basidiomycota / Q000201:enzymology / Q000378:metabolism*; D002712:Chlorides / Q000378:metabolism; D065607:Cytochrome P-450 Enzyme Inhibitors; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D042845:Laccase / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D010100:Oxygen / Q000378:metabolism; D013750:Tetrachloroethylene / Q000378:metabolism*
D002712:Chlorides; D065607:Cytochrome P-450 Enzyme Inhibitors; D004791:Enzyme Inhibitors; D042845:Laccase; D010100:Oxygen; D013750:Tetrachloroethylene