Impact of a new biopesticide produced by Paenibacillus sp. strain B2 on the genetic structure and density of soil bacterial communities.
The effect of paenimyxin, a new biopesticide produced by Paenibacillus sp. strain B2, on the density of soil bacterial communities was assessed by colony counting and by 16S rDNA and nirK quantitative polymerase chain reaction (PCR). Paenimyxin had a negative effect on the bacterial colony-forming unit (CFU) number, which was significantly reduced 2 and 4 days after treatment. The effect of paenimyxin on cultivatable bacteria was negligible 7 days after treatment. Approximately 10(7) 16S rDNA sequences per gram of soil (dry weight) were detected by quantitative PCR in all samples. Paenimyxin did not affect the quantification of 16S rDNA or of the denitrifying bacterial community. In addition, RISA fingerprinting showed that the genetic structure of the bacterial communities was significantly modified 2 days after paenimyxin application at 50 microM and 4 days after treatment at lower concentrations (0.5 and 5 microM). The impact of paenimyxin treatment on the genetic structure of soil bacterial communities was transient, as no effect could be observed after 7, 14 and 28 days when compared with the untreated control.
Selim
Sameh
S
UMR INRA 1088/CNRS 5184/Université de Bourgogne, Plante-Microbe-Environnement CMSE-INRA, 17 rue Sully, BP 86510, 21065 Dijon Cedex, France.
Martin-Laurent
Fabrice
F
Rouard
Nadine
N
Gianinazzi
Silvio
S
van Tuinen
Diederik
D
D001419:Bacteria / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D016172:DNA Fingerprinting; D021903:DNA, Ribosomal Spacer / Q000737:chemistry; D010575:Pesticides / Q000633:toxicity*; D011113:Polymyxins / Q000633:toxicity*; D012336:RNA, Ribosomal, 16S / Q000737:chemistry; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D021903:DNA, Ribosomal Spacer; D010575:Pesticides; D011113:Polymyxins; D012336:RNA, Ribosomal, 16S; C519371:paenimyxin