Signal enhancement of surface plasmon resonance based on gold nanoparticle-antibody complex for immunoassay.
In the immunoassay based on surface plasmon resonance (SPR) system, the signal enhancement was done by means of the conjugate of gold (Au) nanoparticle-antibody fragment. Antibody fragment was prepared for the improved immobilization based on Au-thiol interaction. Through the ellipsometric analysis on surface, the conjugation between Au and antibody fragment was performed in the oriented manner. The optimal fabrication conditions such as concentration and incubation time were determined for the constant size of the fabricated nanoparticle-antibody conjugate. Through the plot of SPR angle difference versus antigen concentration, the linear correlation was achieved, of which the detection limit was 100 fg/ml.
Lee
Woochang
W
Interdisciplinary Program of Integrated Biotechnology, Sogang University, 1 Shinsoo-Dong, Mapo-Gu, Seoul 121-742, Korea.
Oh
Byung-Keun
BK
Kim
Yong-Wan
YW
Choi
Jeong-Woo
JW
D000818:Animals; D000906:Antibodies / Q000737:chemistry; D002417:Cattle; D003543:Cysteamine / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000379:methods*; D013499:Surface Properties
D000906:Antibodies; D012709:Serum Albumin; D013438:Sulfhydryl Compounds; D003543:Cysteamine; D006046:Gold