[STR loci D2S1338 and D19S433 in a population sample from the Lower Silesia region].
Over the past decade, the human identity testing community has established a set of core short tandem repeat (STR) loci that are widely used for DNA typing applications [1]. The present paper analyzed the usefulness of STR D2S1338 and D19S433 loci in paternity testing and human identity establishment. The population study was performed on 1500 individuals, inhabitants of the Lower Silesia region. The DNA samples were amplified simultaneously at 15 STR loci using a multiplex kit AmpFISTR Identifiler PCR Amplification Kit. The amplified products were separated by capillary electrophoresis using an ABI Prism 310 Genetic Analyzer (Applied Biosystems) with the separation medium POP4 according to the manufacturer's recommended protocols. Loci D2S1338 and D19S433 are highly polymorphic [2]. There were no detectable differences from Hardy-Weinberg expectations (HWE) in both the tested loci. The distribution of the observed allele is shown in Figures 1 and 2. The observed and expected homozygotes, the exact test for departures from HWE, probability of discrimination (PD), power of exclusion (PE), matching probability (MP), polymorphism information content (PIC), typical paternity index (TPI) and probability of paternity (PP) were also calculated and presented.
Krzyzanńska
Agnieszka
A
Katedry Medycyny Sadowej, Akademii Medycznej we Wrocławiu. agniek@forensic.am.wroc.pl
Kowalczyk
Elzbieta
E
Markowska
Joanna
J
Dobosz
Tadeusz
T
D016172:DNA Fingerprinting / Q000379:methods*; D053803:Forensic Genetics / Q000379:methods*; D005554:Forensic Medicine / Q000379:methods; D005787:Gene Frequency; D005799:Genes, Dominant; D014644:Genetic Variation; D005828:Genetics, Population; D006801:Humans; D011044:Poland; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012016:Reference Values; D020080:Tandem Repeat Sequences