[Expression of mACh receptor ml-m5 subunits in the vestibular endorgans of rat].
OBJECTIVE
To investigate the expression of mACh receptor ml - m5 subunits in the vestibular endorgans of rat.


METHODS
Gene specific primers used were based upon the rat mACh receptor ml-m5 subunits sequence. Reverse transcription polymerase chain reaction (RT-PCR) was carried out on the vestibular endorgans. The PCR products were directly sequenced.


RESULTS
It was shown that mACh receptor ml - m5 subunits mRNA was detected and sequenced from vestibular endorgans by RT-PCR technique. Sequence analysis of the PCR products confirmed identity to rat mACh receptor ml--m5 subunits cDNA sequence in the predicted region.


CONCLUSIONS
Present data confirmed the expression of ACh receptor ml-m5 subunits in the vestibular endorgans of rat, giving the direct evidence to date that ACh is one of the important neurotransmitters in the peripheral vestibular system of rat.
Cheng
Huanmao
H
Department of Otolaryngology, the First People's Hospital, Jingmen, 438000, China.
Kong
Weijia
W
Yao
Qi
Q
D000818:Animals; D012313:RNA / Q000302:isolation & purification*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011976:Receptors, Muscarinic / Q000378:metabolism*; D014722:Vestibule, Labyrinth / Q000378:metabolism*
D011976:Receptors, Muscarinic; D012313:RNA