title: Apolipoprotein AI-CIII-AIV gene cluster polymorphisms in relation to cholesterol gallstone.
abstract: OBJECTIVE
  To investigate the frequency of variants at Xmn I, Msp I sites of apolipoprotein (Apo), A I-CIII-AIV gene cluster, and its relation to cholesterol gallstones in Chinese patients.
  
  
  METHODS
  Restriction fragment length polymorphisms (RFLP) at Xmn I, Msp I sites of ApoAI-CIII-AIV gene cluster were studied using a polymerase chain reaction (PCR) in 161 patients with cholesterol gallstones and 94 healthy subjects from a Chinese population in Sichuan Province.
  
  
  RESULTS
  In both the cholesterol gallstone group and the healthy control group, X1 and M1 alleles were the major alleles and homozygous X1X1 and M1M1 genotypes were the most frequent. However, the frequency of X2 allele mutation in female patients of the cholesterol gallstones group was significantly higher than that in women in the healthy control group (P<0.05), but no difference was found in the frequency of M2 alleles mutation (P>0.05).
  
  
  CONCLUSIONS
  The data showed that Xmn I RFLP of ApoAI-CIII-AIV gene cluster is associated to some extent with cholesterol gallstones in female Chinese patients.
authors.affiliation: Ya'an People's Hospital, and Sichuan University, China. yyg3969@yahoo.com.cn
authors.forename: You Gui
authors.initials: YG
authors.lastname: Yao
authors.forename: Xiong
authors.initials: X
authors.lastname: Qiu
authors.forename: Ming Kun
authors.initials: MK
authors.lastname: Ma
authors.forename: Dao Sheng
authors.initials: DS
authors.lastname: Pu
authors.forename: Lu Jia
authors.initials: LJ
authors.lastname: Xiao
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016632:Apolipoprotein A-I / Q000235:genetics*; D053305:Apolipoprotein C-III / Q000235:genetics*; D001054:Apolipoproteins A / Q000235:genetics*; D002784:Cholesterol / Q000032:analysis; D005260:Female; D042882:Gallstones / Q000737:chemistry / Q000235:genetics*; D005787:Gene Frequency; D005838:Genotype; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D008297:Male; D008875:Middle Aged; D005810:Multigene Family / Q000235:genetics*; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D016632:Apolipoprotein A-I; D053305:Apolipoprotein C-III; D001054:Apolipoproteins A; C022205:apolipoprotein A-IV; D002784:Cholesterol