title: Improved calcium imaging in transgenic mice expressing a troponin C-based biosensor.
abstract: Fluorescent Ca(2+) indicator proteins (FCIPs) are attractive tools for studying Ca(2+) dynamics in live cells. Here we describe transgenic mouse lines expressing a troponin C (TnC)-based biosensor. The biosensor is widely expressed in neurons and has improved Ca(2+) sensitivity both in vitro and in vivo. This allows FCIP-based two-photon Ca(2+) imaging of distinct neurons and their dendrites in vivo, and opens a new avenue for structure-function analysis of intact neuronal circuits.
authors.affiliation: Max Planck Institute of Neurobiology, Am Klopferspitz 18, 82152 Martinsried, Germany.
authors.forename: Nicola
authors.initials: N
authors.lastname: Heim
authors.forename: Olga
authors.initials: O
authors.lastname: Garaschuk
authors.forename: Michael W
authors.initials: MW
authors.lastname: Friedrich
authors.forename: Marco
authors.initials: M
authors.lastname: Mank
authors.forename: Ruxandra I
authors.initials: RI
authors.lastname: Milos
authors.forename: Yury
authors.initials: Y
authors.lastname: Kovalchuk
authors.forename: Arthur
authors.initials: A
authors.lastname: Konnerth
authors.forename: Oliver
authors.initials: O
authors.lastname: Griesbeck
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D001921:Brain / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D003712:Dendrites / Q000378:metabolism; D005260:Female; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D009474:Neurons / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D019209:Troponin C / Q000235:genetics / Q000378:metabolism*
chemical_list: C485182:Cyan Fluorescent Protein; D019209:Troponin C; D049452:Green Fluorescent Proteins; D002118:Calcium