title: Self-phosphorylation modulates the gating of rat liver gap junction channels: a nonstationary noise analysis.
abstract: The effect of phosphorylation on the gating of rat liver gap junction hemichannels (Cx 32) has been investigated. It has been shown that self-phosphorylation of rat liver Cx 32 protein reduces the permeability of proteoliposomes as well the current flowing through multichannels in lipid bilayer membrane (BLM). The low frequency power spectral density analyses of nonstationary noise evolved due to the gating of Cx 32 multichannels demonstrated that self-phosphorylation modulated the channel functioning. A clear change in the power spectrum slopes (alpha) of the nonstationary noise profiles confirmed the modulation of the channel dynamics due to self-regulation.
authors.affiliation: Department of Biophysics, University of Delhi South campus, New Delhi-110021, India. para_ghosh@rediffmail.com
authors.forename: Paramita
authors.initials: P
authors.lastname: Ghosh
mesh_terms: D000818:Animals; D017630:Connexins / Q000032:analysis / Q000378:metabolism*; D008099:Liver / Q000737:chemistry / Q000378:metabolism*; D010539:Permeability; D010766:Phosphorylation; D011510:Proteolipids / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D000097002:Gap Junction beta-1 Protein
chemical_list: D017630:Connexins; D011510:Proteolipids; D000097002:Gap Junction beta-1 Protein; C020485:proteoliposomes