[Apoptosis and gene FasL expression induced by carbon disulfide in rat sertoli cells].
OBJECTIVE
To study apoptosis and gene FasL expression induced by carbon disulfide in sertoli cells of male rats.


METHODS
Sertoli cells were exposed to different concentrations of CS(2) (0, 0.36, 0.72, 1.44 micromol/ml) for 24 hours. Survival rate, apoptosis rate, expression level of gene FasL were measured using MTT, FCM, and RT-PCR methods respectively.


RESULTS
Sertoli cell survival rate decreased as the concentration of CS(2) increased. The survival rate (73.34% +/- 1.39%) was significantly lower than the control group (99.98% +/- 5.48%) when the concentration of CS(2) > or = 1.44 micromol/ml (P < 0.05). Apoptosis rate increased as the CS(2) concentration increased. Apoptosis rate (7.93% +/- 0.43%) was significantly higher when the concentration of CS(2) > or = 1.44 micromol/ml (P < 0.05). Expression level of the FasL significantly increased as the concentrations of CS(2) (P < 0.05).


CONCLUSIONS
CS(2) is cytotoxic to sertoli cells. It could cause apoptosis of sertoli cells.
Deng
Jing
J
Department of Labor Health & Environment Health, Tongji Medical College of Huazhong University of Science & Technology, Wuhan 430030, China.
Chen
Guo-Yuan
GY
Ji
Jia-Jia
JJ
Tan
Hao
H
Wang
Song
S
Hu
Feng-Xian
FX
Chai
Lian-Hua
LH
Liu
Si-Hai
SH
Liu
Wei-Dong
WD
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002246:Carbon Disulfide / Q000633:toxicity*; D002460:Cell Line; D002470:Cell Survival; D053222:Fas Ligand Protein / Q000378:metabolism*; D008297:Male; D051381:Rats; D012708:Sertoli Cells / Q000187:drug effects* / Q000378:metabolism*; D013737:Testis / Q000166:cytology
D053222:Fas Ligand Protein; C505662:Faslg protein, rat; D002246:Carbon Disulfide