title: [Simultaneous determination of eight organic acids in Fructus mume by RP-HPLC].
abstract: OBJECTIVE
  To develop an HPLC method for the simultaneous separation and determination of oxalic acid (OA), tartaric acid(TA), malic acid(MA), vitamin C (VC), lactic acid (LA), acetic acid (AA) citric acid (CA) and succinic acid (SA) in Fructus mume.
  
  
  METHODS
  Analytical column was Zorbax Eclipse XDB C18. Mobile phase was 0.5% (NH4) H2PO4 aqueous solution and detection wavelength was 214 nm. The flow rate of mobile phase was 0.5 mL x min(-1).
  
  
  RESULTS
  The regression equations (pH 2. 8, adjusted with phosphoric acid) of eight constituents have been established, r = 0.999 7, 0. 999 8, 0.999 2, 0.999 6, 0.999 1, 0.999 5, 0.999 8, 0.999 2 respectively. Meanwhile, the content and proportion relationship of eight organic acids in Fructus mume which yielded in Fujian (China) were investigated.
  
  
  CONCLUSIONS
  This method was simple, accuracy and quick. The method can be used for the purpose of routine analysis and the quality control of a botanic (Fructus mume) containing these organic acid components.
authors.affiliation: College of Chemistry and Materials Science, Shaaxi Normal University, Xi'an 710062, China. chzhg@snnu.edu.cn
authors.forename: Zhan-Guo
authors.initials: ZG
authors.lastname: Chen
authors.forename: Bo-Ti
authors.initials: BT
authors.lastname: En
authors.forename: Zhi-Qi
authors.initials: ZQ
authors.lastname: Zhang
mesh_terms: D019342:Acetic Acid / Q000032:analysis; D001205:Ascorbic Acid / Q000032:analysis; D002264:Carboxylic Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019343:Citric Acid / Q000032:analysis; D005638:Fruit / Q000737:chemistry; D019344:Lactic Acid / Q000032:analysis; D008293:Malates / Q000032:analysis; D019815:Oxalic Acid / Q000032:analysis; D010946:Plants, Medicinal / Q000737:chemistry*; D027861:Prunus / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D019802:Succinic Acid / Q000032:analysis; D013644:Tartrates / Q000032:analysis
chemical_list: D002264:Carboxylic Acids; D008293:Malates; D013644:Tartrates; D019343:Citric Acid; D019344:Lactic Acid; C030298:malic acid; D019815:Oxalic Acid; D019802:Succinic Acid; D001205:Ascorbic Acid; D019342:Acetic Acid; C029768:tartaric acid