title: Detection of SRV/D shedding in body fluids of cynomolgus macaques and comparison of partial gp70 sequences in SRV/D-T isolates.
abstract: We previously reported the isolation of a novel subtype of SRV/D-Tsukuba (SRV/D-T) from two cynomolgus monkeys (Macaca facicularis) in the breeding colony of Tsukuba Primate Research Center (TPRC). We surveyed for SRV/D infection in the TPRC cynomolgus colony using SRV/D-specific PCR primer sets designed based on the entire gag region sequence. The only SRV/D subtype detected in the colony was SRV/D-T with a positive infection rate of 22.4% (n = 49). It has been reported that the mode of transmission of SRV/D is via contact with virus shed in the body fluids. In this report, to investigate the infection route of SRV/D-T in monkeys at TPRC, we performed virus isolation and PCR for detection of the SRV/D genome from peripheral blood mononuclear cells (PBMCs), plasma, saliva, urine, and feces. Virus isolation and PCR detection were positive in plasma, saliva, urine, and fecal samples from all monkeys on which virus was isolated from PBMCs. This suggests that the spread of SRV/D-T infection in TPRC is via contact with virus shed in saliva, urine, and/or feces. Also, comparison of sequences of gp70 on multiple SRV/D-T isolates revealed that there was little intra- and inter-monkey variation, suggesting that SRV/D-T is fairly stable.
authors.affiliation: Tsukuba Primate Research Center, National Institute of Biomedical Innovation, 1 Hachimanndai, Tsukuba 305-0843, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Hara
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Kikuchi
authors.forename: Tetsutaro
authors.initials: T
authors.lastname: Sata
authors.forename: Noriko
authors.initials: N
authors.lastname: Nakajima
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Ami
authors.forename: Yuko
authors.initials: Y
authors.lastname: Sato
authors.forename: Keiko
authors.initials: K
authors.lastname: Tanaka
authors.forename: Toyoko
authors.initials: T
authors.lastname: Narita
authors.forename: Fumiko
authors.initials: F
authors.lastname: Ono
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Akari
authors.forename: Keiji
authors.initials: K
authors.lastname: Terao
authors.forename: Ryozaburo
authors.initials: R
authors.lastname: Mukai
mesh_terms: D000818:Animals; D001483:Base Sequence; D001826:Body Fluids / Q000821:virology*; D005243:Feces / Q000821:virology; D005260:Female; D006023:Glycoproteins / Q000097:blood* / Q000235:genetics / Q000652:urine*; D018445:Infectious Disease Transmission, Vertical; D008252:Macaca fascicularis; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000097:blood* / Q000652:urine*; D012192:Retroviridae Infections / Q000635:transmission / Q000821:virology; D015301:Retroviruses, Simian / Q000145:classification / Q000302:isolation & purification / Q000502:physiology*; D012463:Saliva / Q000821:virology; D014412:Tumor Virus Infections / Q000635:transmission / Q000821:virology; D014764:Viral Proteins / Q000097:blood* / Q000235:genetics / Q000652:urine*; D017201:Virus Shedding / Q000502:physiology*
chemical_list: D006023:Glycoproteins; D010446:Peptide Fragments; D014764:Viral Proteins; C095282:glycoprotein gp70, simian retrovirus-2