title: Regulation and enzymatic basis of bone resorption by human osteoclasts.
abstract: Although much has been learned recently of the mechanisms that regulate osteoclastic differentiation, much less is known of the means through which their resorptive activity is controlled. This is especially so for human osteoclasts. We have recently developed an assay that allows us to measure resorptive activity while minimizing confounding effects on differentiation by optimizing osteoclastogenesis, so that measurable resorption occurs over a short period, and by relating resorption in each culture during the test period to the resorption that had occurred in the same culture in a prior control period. In the present study, we found that RANKL (receptor activator of nuclear factor kappaB ligand) strongly stimulated the release of CTX-I (C-terminal telopeptide degradation product of type I collagen) by osteoclasts over a similar range to that over which it induces osteoclastic differentiation, consistent with a distinct action on osteoclastic function. CT (calcitonin) dose-dependently inhibited bone resorption, whereas PTH (parathyroid hormone), IL (interleukin)-1, TNF-alpha (tumour necrosis factor-alpha), IL-6, IL-8, VEGF (vascular endothelial growth factor), MCP-1 (monocyte chemoattractant protein-1), MIP-1gamma (macrophage inflammatory protein-1gamma), IFN (interferon)-gamma and dibutyryl cGMP had no significant effect. Ca(2+), cyclosporin A, IFN-beta and dibutyryl cAMP all strongly suppressed resorption. Bone resorption was also strongly suppressed by alendronate, the cysteine protease inhibitor E64 and the cathepsin K inhibitor MV061194. Inhibitors of MMPs (matrix metalloproteinases) had no effect on CTX-I release. Moreover, the release of the MMP-derived collagen fragment ICTP (C-terminal cross-linked telopeptide of type I collagen) represented less that 0.01% of the quantity of CTX-I released in our cultures. This suggests that MMPs make, at most, a very small contribution to the bone-resorptive activity of osteoclasts.
authors.affiliation: Department of Cellular Pathology, St George's, University of London, Cranmer Terrace, Tooting, London, UK.
authors.forename: Karen
authors.initials: K
authors.lastname: Fuller
authors.forename: Barrie
authors.initials: B
authors.lastname: Kirstein
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Chambers
mesh_terms: D000328:Adult; D050071:Bone Density Conservation Agents / Q000494:pharmacology; D001862:Bone Resorption / Q000201:enzymology*; D002116:Calcitonin / Q000494:pharmacology; D056657:Cathepsin K; D002403:Cathepsins / Q000502:physiology*; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D016207:Cytokines / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D006706:Homeostasis / Q000502:physiology; D006801:Humans; D008297:Male; D020782:Matrix Metalloproteinases / Q000502:physiology*; D008875:Middle Aged; D010010:Osteoclasts / Q000201:enzymology*; D010455:Peptides / Q000378:metabolism; D053245:RANK Ligand / Q000494:pharmacology
chemical_list: D050071:Bone Density Conservation Agents; D024042:Collagen Type I; D016207:Cytokines; D004791:Enzyme Inhibitors; D010455:Peptides; D053245:RANK Ligand; C505688:TNFSF11 protein, human; C052929:collagen type I trimeric cross-linked peptide; D002116:Calcitonin; D002403:Cathepsins; C534130:CTSK protein, human; D056657:Cathepsin K; D020782:Matrix Metalloproteinases