Minimization of PCR efficiency differences between standards and samples through dilution of PCR amplicons in reverse transcription buffer.
Simard
Marc
M
Ontogeny and Reproduction Unit, Centre Hospitalier Universitaire de Québec, Pavillon Centre Hospitalier de l'Université Laval, Sainte-Foy, Qué., Canada.
Boucher
Eric
E
Provost
Pierre R
PR
Tremblay
Yves
Y
D002021:Buffers; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012015:Reference Standards; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods* / Q000592:standards
D002021:Buffers; D012333:RNA, Messenger