title: Aeromonas salmonicida toxin AexT has a Rho family GTPase-activating protein domain.
abstract: The N terminus of the Aeromonas salmonicida ADP-ribosylating toxin AexT displays in vitro GTPase-activating protein (GAP) activity for Rac1, CDC42, and RhoA. HeLa cells transfected with the AexT N terminus exhibit rounding and actin disordering. We propose that the Aeromonas salmonicida AexT toxin is a novel member of the growing family of bacterial RhoGAPs.
authors.affiliation: Department of Biological Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904 Israel.
authors.forename: Yael
authors.initials: Y
authors.lastname: Litvak
authors.forename: Zvi
authors.initials: Z
authors.lastname: Selinger
mesh_terms: D036002:ADP Ribose Transferases / Q000737:chemistry* / Q000378:metabolism; D048409:Aeromonas salmonicida / Q000201:enzymology* / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001427:Bacterial Toxins / Q000737:chemistry* / Q000378:metabolism; D020690:GTPase-Activating Proteins / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D014162:Transfection; D020764:cdc42 GTP-Binding Protein / Q000378:metabolism; D020830:rac1 GTP-Binding Protein / Q000378:metabolism; D020742:rhoA GTP-Binding Protein / Q000378:metabolism
chemical_list: D001427:Bacterial Toxins; D020690:GTPase-Activating Proteins; C107953:RAC1 protein, human; C085379:rho GTPase-activating protein; C066737:RHOA protein, human; D036002:ADP Ribose Transferases; D020764:cdc42 GTP-Binding Protein; D020830:rac1 GTP-Binding Protein; D020742:rhoA GTP-Binding Protein