title: The Enterococcus hirae Mur-2 enzyme displays N-acetylglucosaminidase activity.
abstract: Enterococcus hirae produces two autolytic enzymes named Mur-1 and Mur-2, both previously described as N-acetylmuramidases. We used tandem mass spectrometry to show that Mur-2 in fact displays N-acetylglucosaminidase activity. This result reveals that Mur-2 and its counterparts studied to date, which are members of glycosyl hydrolase family 73 from the CAZy (Carbohydrate-Active enZyme) database, display the same catalytic activity.
authors.affiliation: INSERM, U655-LRMA, Centre de Recherches Biomédicales des Cordeliers, Paris, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Eckert
authors.forename: Sophie
authors.initials: S
authors.lastname: Magnet
authors.forename: Stéphane
authors.initials: S
authors.lastname: Mesnage
mesh_terms: D000118:Acetylglucosaminidase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D000595:Amino Acid Sequence; D020134:Catalytic Domain; D016983:Enterococcus / Q000201:enzymology*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D013379:Substrate Specificity
chemical_list: D000118:Acetylglucosaminidase