The fragile X mental retardation protein-RNP granules show an mGluR-dependent localization in the post-synaptic spines.
The localization of RNA/mRNA in dendrites plays a role in both local and temporal regulation of protein synthesis, which is required for certain forms of synaptic plasticity. A key molecule in these processes is the fragile X mental retardation protein (FMRP). Using in situ hybridization coupled to immunofluorescence confocal microscopy, we find that the FMRP-RNP particle contains alphaCaMKII and BC1 RNAs as well as Staufen and CPEB proteins. Furthermore, following mGluR activation, the FMRP-mRNP complex moves into spines as shown by co-localization with the PSD-95 and Shank proteins. This study shows, for the first time, that the translationally inactive FMRP-mRNP complex relocates into neuronal spines after stimulation and that de novo protein synthesis mainly contributes to the pool of FMRP at synapses.
Ferrari
Francesca
F
Fondazione Santa Lucia, Rome, Italy.
Mercaldo
Valentina
V
Piccoli
Giovanni
G
Sala
Carlo
C
Cannata
Stefano
S
Achsel
Tilmann
T
Bagni
Claudia
C
D000818:Animals; D001921:Brain / Q000166:cytology; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D006224:Cricetinae; D049229:Dendritic Spines / Q000187:drug effects / Q000378:metabolism*; D000076122:Disks Large Homolog 4 Protein; D004622:Embryo, Mammalian; D005455:Fluorescent Antibody Technique / Q000379:methods; D051860:Fragile X Messenger Ribonucleoprotein 1 / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000502:physiology; D051528:Guanylate Kinases; D017403:In Situ Hybridization; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D008734:Methoxyhydroxyphenylglycol / Q000031:analogs & derivatives / Q000494:pharmacology; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000166:cytology*; D016601:RNA-Binding Proteins / Q000378:metabolism; D018094:Receptors, Metabotropic Glutamate / Q000037:antagonists & inhibitors / Q000502:physiology*; D013997:Time Factors; D014162:Transfection; D016875:tau Proteins / Q000378:metabolism
D000076122:Disks Large Homolog 4 Protein; D051860:Fragile X Messenger Ribonucleoprotein 1; D051528:Guanylate Kinases; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D008734:Methoxyhydroxyphenylglycol; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins; D018094:Receptors, Metabotropic Glutamate; D016875:tau Proteins; C501447:Dlg4 protein, mouse; C079240:Mtap2 protein, mouse; C492187:Stau1 protein, mouse; C010117:3,4-dihydroxyphenylglycol