The effect of pleiotrophin signaling on adipogenesis.
Pleiotrophin (PTN) plays diverse roles in cell growth and differentiation. In this investigation, we demonstrate that PTN plays a negative role in adipogensis and that glycogen synthase kinase 3beta (GSK-3beta) and beta-catenin are involved in the regulation of PTN-mediated preadipocyte differentiation. Knocking down the expression of PTN using siRNA resulted in an increase in phospho-GSK-3beta expression, and the accumulation of nuclear beta-catenin, which are critical downstream signaling proteins for both the PTN and Wnt signaling pathways. Our investigation suggests that there is a PTN/PI3K/AKT/GSK-3beta/beta-catenin signaling pathway, which cross-talks with the Wnt/Fz/GSK-3beta/beta-catenin pathway and negatively regulates adipogenesis.
Gu
Dayong
D
Life Science Division, Graduate School at Shenzhen, Tsinghua University, Room 407, Building L, Tsinghua Campus, University Town, Shenzhen, Guangdong 518055, PR China.
Yu
Bing
B
Zhao
Chen
C
Ye
Wenbin
W
Lv
Qing
Q
Hua
Zhong
Z
Ma
Jiangan
J
Zhang
Yaou
Y
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects; D016207:Cytokines / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D003907:Dexamethasone / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects; D020869:Gene Expression Profiling; D051379:Mice; D008954:Models, Biological; D047495:PPAR gamma / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction; D051153:Wnt Proteins / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism
C495267:CTNNB1 protein, mouse; D002352:Carrier Proteins; D016207:Cytokines; D047495:PPAR gamma; D034741:RNA, Small Interfering; D051153:Wnt Proteins; D051176:beta Catenin; C066845:pleiotrophin; D003907:Dexamethasone