title: A novel method to fabricate patterned bilayer lipid membranes.
abstract: We introduce a new method for forming tethered bilayer lipid membranes on surfaces patterned using a photocleavable self-assembled monolayer (SAM). A SAM terminated with a hydrophobic fluorocarbon residue was bound to a gold surface through a link containing a photocleavable ortho-nitrobenzyl moiety. Hydrophilic regions were produced by irradiation with soft UV (365 nm) through a photomask. The patterned surface was characterized by scanning electron microscopy and electrochemical impedance spectroscopy. Tethered bilayer lipid membranes with well-defined bilayer and monolayer regions were then formed by exposure to egg PC vesicles. The membranes had resistance and capacitance values of 0.52 MOmega.cm2 and 0.83 microF.cm-2, respectively.
authors.affiliation: School of Physics and Astronomy, and Self-Organising Molecular Systems (SOMS) Centre, University of Leeds, LS2 9JT Leeds, United Kingdom.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Han
authors.forename: Kevin
authors.initials: K
authors.lastname: Critchley
authors.forename: Lixin
authors.initials: L
authors.lastname: Zhang
authors.forename: Singh N D
authors.initials: SN
authors.lastname: Pradeep
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Bushby
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Evans
mesh_terms: D003432:Cross-Linking Reagents; D004563:Electrochemistry; D006046:Gold; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry; D008722:Methods; D008855:Microscopy, Electron, Scanning; D010713:Phosphatidylcholines; D013057:Spectrum Analysis; D013499:Surface Properties
chemical_list: D003432:Cross-Linking Reagents; D008051:Lipid Bilayers; D008081:Liposomes; D010713:Phosphatidylcholines; D006046:Gold