Recent developments in sample preparation for chromatographic analysis of carbohydrates.
Carbohydrates are a very important group of compounds due to their roles as structural materials, sources of energy, biological functions and environmental analytes; they are characterized by their structural diversity and the high number of isomers they present. While many advances have been made in carbohydrate analysis, the sample preparation remains difficult. This review aims to summarize the most important treatments which have been recently developed to be applied prior to the analysis of carbohydrates by chromatographic techniques. Due to the multiplicity of structures and matrices, many different techniques are required for clean-up, fractionation and derivatization. A number of new techniques which could be potentially adequate for carbohydrate characterization have also been revised.
Sanz
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ML
Instituto de Química Orgánica General, Consejo Superior de Investigaciones Científicas (CSIC), C/Juan de la Cierva, 3 E-28006 Madrid, Spain.
Martínez-Castro
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I
D053000:Analytic Sample Preparation Methods / Q000379:methods*; D002241:Carbohydrates / Q000032:analysis* / Q000737:chemistry; D002845:Chromatography / Q000379:methods*; D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D020374:Chromatography, Micellar Electrokinetic Capillary; D006358:Hot Temperature; D006868:Hydrolysis; D011312:Pressure; D052616:Solid Phase Extraction; D015003:Yeasts / Q000378:metabolism
D002241:Carbohydrates