The binding avidity of a nanoparticle-based multivalent targeted drug delivery platform.
Dendrimer-based anticancer nanotherapeutics containing approximately 5 folate molecules have shown in vitro and in vivo efficacy in cancer cell targeting. Multivalent interactions have been inferred from observed targeting efficacy, but have not been experimentally proven. This study provides quantitative and systematic evidence for multivalent interactions between these nanodevices and folate-binding protein (FBP). A series of the nanodevices were synthesized by conjugation with different amounts of folate. Dissociation constants (K(D)) between the nanodevices and FBP measured by SPR are dramatically enhanced through multivalency ( approximately 2,500- to 170,000-fold). Qualitative evidence is also provided for a multivalent targeting effect to KB cells using flow cytometry. These data support the hypothesis that multivalent enhancement of K(D), not an enhanced rate of endocytosis, is the key factor resulting in the improved biological targeting by these drug delivery platforms.
Hong
Seungpyo
S
Program in Macromolecular Science and Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
Leroueil
Pascale R
PR
Majoros
István J
IJ
Orr
Bradford G
BG
Baker
James R
JR
Banaszak Holl
Mark M
MM
D000970:Antineoplastic Agents / Q000008:administration & dosage; D002352:Carrier Proteins / Q000378:metabolism; D045744:Cell Line, Tumor; D050091:Dendrimers / Q000627:therapeutic use; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry*; D016503:Drug Delivery Systems; D005434:Flow Cytometry; D058973:Folate Receptors, GPI-Anchored; D005492:Folic Acid / Q000737:chemistry; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry / Q000627:therapeutic use*; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000378:metabolism; D020349:Surface Plasmon Resonance
D000970:Antineoplastic Agents; D002352:Carrier Proteins; D050091:Dendrimers; D004337:Drug Carriers; D058973:Folate Receptors, GPI-Anchored; D011956:Receptors, Cell Surface; D005492:Folic Acid