title: The structure of human collapsin response mediator protein 2, a regulator of axonal growth.
abstract: Axonal growth cone guidance is a central process in nervous system development and repair. Collapsin response mediator protein 2 (CRMP-2) is a neurite extension-promoting neuronal cytosolic molecule involved in the signalling of growth inhibitory cues from external stimuli, such as semaphorin 3A and the myelin-associated glycoprotein. We have determined the crystal structure of human tetrameric CRMP-2, which is structurally related to the dihydropyriminidases; however, the active site is not conserved. The wealth of earlier functional mapping data for CRMP-2 are discussed in light of the three-dimensional structure of the protein. The differences in oligomerisation interfaces between CRMP-1 and CRMP-2 are used to model CRMP-1/2 heterotetramers.
authors.affiliation: Structural Genomics Consortium, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden.
authors.forename: Pål
authors.initials: P
authors.lastname: Stenmark
authors.forename: Derek
authors.initials: D
authors.lastname: Ogg
authors.forename: Susanne
authors.initials: S
authors.lastname: Flodin
authors.forename: Alex
authors.initials: A
authors.lastname: Flores
authors.forename: Tetyana
authors.initials: T
authors.lastname: Kotenyova
authors.forename: Tomas
authors.initials: T
authors.lastname: Nyman
authors.forename: Pär
authors.initials: P
authors.lastname: Nordlund
authors.forename: Petri
authors.initials: P
authors.lastname: Kursula
mesh_terms: D000818:Animals; D003001:Cloning, Molecular / Q000379:methods; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000737:chemistry* / Q000235:genetics; D051379:Mice; D008958:Models, Molecular; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000235:genetics; D014176:Protein Biosynthesis / Q000502:physiology; D000098855:Collapsin Response Mediator Protein 1
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; C502872:Crmp1 protein, mouse; D000098855:Collapsin Response Mediator Protein 1; C095114:collapsin response mediator protein-2