The structure of human collapsin response mediator protein 2, a regulator of axonal growth.
Axonal growth cone guidance is a central process in nervous system development and repair. Collapsin response mediator protein 2 (CRMP-2) is a neurite extension-promoting neuronal cytosolic molecule involved in the signalling of growth inhibitory cues from external stimuli, such as semaphorin 3A and the myelin-associated glycoprotein. We have determined the crystal structure of human tetrameric CRMP-2, which is structurally related to the dihydropyriminidases; however, the active site is not conserved. The wealth of earlier functional mapping data for CRMP-2 are discussed in light of the three-dimensional structure of the protein. The differences in oligomerisation interfaces between CRMP-1 and CRMP-2 are used to model CRMP-1/2 heterotetramers.
Stenmark
Pål
P
Structural Genomics Consortium, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden.
Ogg
Derek
D
Flodin
Susanne
S
Flores
Alex
A
Kotenyova
Tetyana
T
Nyman
Tomas
T
Nordlund
Pär
P
Kursula
Petri
P
D000818:Animals; D003001:Cloning, Molecular / Q000379:methods; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000737:chemistry* / Q000235:genetics; D051379:Mice; D008958:Models, Molecular; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000235:genetics; D014176:Protein Biosynthesis / Q000502:physiology; D000098855:Collapsin Response Mediator Protein 1
D036341:Intercellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; C502872:Crmp1 protein, mouse; D000098855:Collapsin Response Mediator Protein 1; C095114:collapsin response mediator protein-2