Quantitative determination of isotope ratios from experimental isotopic distributions.
Isotope variability due to natural processes provides important information for studying a variety of complex natural phenomena from the origins of a particular sample to the traces of biochemical reaction mechanisms. These measurements require high-precision determination of isotope ratios of a particular element involved. Isotope ratio mass spectrometers (IRMS) are widely employed tools for such a high-precision analysis, which have some limitations. This work aims at overcoming the limitations inherent to IRMS by estimating the elemental isotopic abundance from the experimental isotopic distribution. In particular, a computational method has been derived that allows the calculation of 13C/12C ratios from the whole isotopic distributions, given certain caveats, and these calculations are applied to several cases to demonstrate their utility. The limitations of the method in terms of the required number of ions and S/N ratio are discussed. For high-precision estimates of the isotope ratios, this method requires very precise measurement of the experimental isotopic distribution abundances, free from any artifacts introduced by noise, sample heterogeneity, or other experimental sources.
Kaur
Parminder
P
Department of Electrical and Computer Engineering, Boston University, 8 St. Mary's St, Boston, Massachusetts 02215, USA.
O'Connor
Peter B
PB
D007554:Isotopes / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*
D007554:Isotopes