title: Solution structure of the twelfth cysteine-rich ligand-binding repeat in rat megalin.
abstract: Megalin, an approx. 600 kDa transmembrane glycoprotein that acts as multi-ligand transporter, is a member of the low density lipoprotein receptor gene family. Several cysteine-rich repeats, each consisting of about 40 residues, are responsible for the multispecific binding of ligands. The solution structure of the twelfth cysteine-rich ligand-binding repeat with class A motif found in megalin features two short beta-strands and two helical turns, yielding the typical fold with a I-III, II-V and IV-VI disulfide bridge connectivity pattern and a calcium coordination site at the C-terminal end. The resulting differences in electrostatic surface potential compared to other ligand-binding modules of this gene family, however, may be responsible for the functional divergence.
authors.affiliation: Institute of Biophysical Chemistry, Center for Biomolecular Magnetic Resonance, J.W. Goethe-University of Frankfurt, 60439 Frankfurt, Germany.
authors.forename: Christian A
authors.initials: CA
authors.lastname: Wolf
authors.forename: Felician
authors.initials: F
authors.lastname: Dancea
authors.forename: Meichen
authors.initials: M
authors.lastname: Shi
authors.forename: Veronika
authors.initials: V
authors.lastname: Bade-Noskova
authors.forename: Heinz
authors.initials: H
authors.lastname: Rüterjans
authors.forename: Dontscho
authors.initials: D
authors.lastname: Kerjaschki
authors.forename: Christian
authors.initials: C
authors.lastname: Lücke
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D002118:Calcium / Q000737:chemistry; D003545:Cysteine / Q000737:chemistry*; D008024:Ligands; D026561:Low Density Lipoprotein Receptor-Related Protein-2 / Q000737:chemistry*; D008969:Molecular Sequence Data; D017510:Protein Folding; D017433:Protein Structure, Secondary; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid; D012996:Solutions / Q000737:chemistry; D055672:Static Electricity
chemical_list: D008024:Ligands; D026561:Low Density Lipoprotein Receptor-Related Protein-2; D012996:Solutions; D003545:Cysteine; D002118:Calcium