Technological platforms for microarray gene expression profiling.
By using gene microarray technology, scientists can determine in a single experiment, the expression levels of thousands of genes within a given sample. DNA microarray technology is evolving rapidly and there are now numerous high-density platforms available which differ in terms of probe content, design, deposition technology, labeling and hybridization protocols. However, two major platforms for high-density microarray manufacture are in common use. The first utilizes robotic deposition or "spotting" of DNA molecules, while the second uses short oligonucleotides synthesized in situ.
Mandruzzato
Susanna
S
Oncology Section, Department Oncological and Surgical Sciences, University of Padova, Via Gattamelata 64, Italy. susanna.mandruzzato@unipd.it
D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000378:metabolism; D020869:Gene Expression Profiling; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods*; D009841:Oligonucleotides / Q000737:chemistry; D012313:RNA / Q000737:chemistry
D018076:DNA, Complementary; D009841:Oligonucleotides; D012313:RNA