title: The 8-epimer of prostaglandin F(2alpha), a marker of lipid peroxidation and oxidative stress, is decreased in the nipple aspirate fluid of women with breast cancer.
abstract: Breast cancer (BC), a worldwide disease with increasing incidence, develops from ductal/lobular epithelium. Nipple aspirate fluid (NAF), secreted from the breast ducts and lobules, can be analyzed to assess breast metabolic activity. Whether lipid peroxidation in the mammary gland promotes or prevents tumorigenesis is unclear. Malondialdehyde (MDA) and the 8-epimer of Prostaglandin F(2alpha) (8-iso-PGF(2alpha)), two lipid peroxidation markers, were studied in milk (n = 10), NAF (n = 140) and plasma (n = 35) samples. MDA was detected in all plasma, in 80% of milk samples and in 95% of NAF samples. MDA levels in NAF and plasma were significantly higher than in milk (p = 0.016 and p = 0.029, respectively). We found no significant difference between levels of MDA in NAF samples from BC patients compared to healthy controls. 8-iso-PGF(2alpha) was detectable in all samples. 8-iso-PGF(2alpha) median levels in NAF were significantly higher than in both milk and plasma (p < 0.0001). The highest 8-iso-PGF(2alpha) levels were found in NAF from healthy women, significantly higher than in women with BC (p < 0.0001). No significant differences were found in both markers after the age-adjustment. High levels of lipid peroxidation products in NAF suggest their in situ production in the nonlactating breast. Active lipid peroxidation may have a physiologic role in the normal mammary gland. Lower levels of 8-iso-PGF(2alpha) in NAF from BC patients suggest altered production of arachidonic acid metabolites during breast carcinogenesis.
authors.affiliation: Institute of Histology and Laboratory Analysis, University "Carlo Bo," Urbino, Italy. f.mannello@uniurb.it
authors.forename: Ferdinando
authors.initials: F
authors.lastname: Mannello
authors.forename: Gaetana A M
authors.initials: GA
authors.lastname: Tonti
authors.forename: Silvia
authors.initials: S
authors.lastname: Pagliarani
authors.forename: Serena
authors.initials: S
authors.lastname: Benedetti
authors.forename: Franco
authors.initials: F
authors.lastname: Canestrari
authors.forename: Weizhu
authors.initials: W
authors.lastname: Zhu
authors.forename: Wenyi
authors.initials: W
authors.lastname: Qin
authors.forename: Edward R
authors.initials: ER
authors.lastname: Sauter
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers; D001943:Breast Neoplasms / Q000378:metabolism*; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000032:analysis; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000032:analysis; D008875:Middle Aged; D008895:Milk, Human / Q000737:chemistry; D009558:Nipples / Q000737:chemistry* / Q000378:metabolism; D018384:Oxidative Stress
chemical_list: D015415:Biomarkers; C075750:8-epi-prostaglandin F2alpha; D008315:Malondialdehyde; D015237:Dinoprost