[Gene expression of tumor cells both in vitro and in vivo enhanced by integrin-targeting adenovirus vector].
OBJECTIVE
To construct an efficient recombinant viral vector for gene therapy.


METHODS
First-generation adenovirus (Ad) vector was modified with the RGD peptide inserted into the fiber. Both in vitro and in vivo experiments of gene expression in different tumor cells with conventional and recombinant vectors were conducted. RT-PCR was used for detecting the expression of coxackievirus and adenovirus receptor and integrin at the surface of Meth-A cells.


RESULTS
Fiber-mutant adenovirus vector showed a notably enhanced gene expression in A2058, B16BL6, OV-HM, and Meth-A tumor cells compared with that of conventional ones. In vivo study carried out using Meth-A tumor-bearing mice also demonstrated that the intra-tumoral injection of recombinant adenovirus induced strong gene expression in these CAR-deficient tumor cells.


CONCLUSIONS
The recombinant vector can be a promising one for effective cancer gene therapy.
Gao
Jian-qing
JQ
Institute of Pharmaceutics, Zhejiang University, Hangzhou 310031, China. gaojianqing1029@yahoo.com.cn
Chen
Hai-liang
HL
Nakagawa
Shinsaku
S
Mizuguchi
Hiroyuki
H
Liang
Wen-quan
WQ
D000256:Adenoviridae / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D045744:Cell Line, Tumor; D004770:Enterovirus / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D016023:Integrins / Q000235:genetics* / Q000378:metabolism; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D009154:Mutation; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D009842:Oligopeptides / Q000235:genetics; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
D016023:Integrins; D009842:Oligopeptides; D011991:Receptors, Virus; D049452:Green Fluorescent Proteins; C047981:arginyl-glycyl-aspartic acid; D008156:Luciferases