Cryopreservation of hematopoietic stem cells.
Stem cell transplantation represents a critical approach for the treatment of many malignant and non-malignant diseases. The foundation for these approaches is the ability to cryopreserve marrow cells for future use. This technique is routinely employed in all autologous settings and is critical for cord blood transplantation. A variety of cryopreservatives have been used with multiple freezing and thawing techniques as outlined in the later chapters. Freezing efficiency has been proven repeatedly and the ability of long-term stored marrow to repopulate has been established. Standard approaches outlined here are used in many labs as the field continues to evolve.
Berz
David
D
Roger Williams Medical Center, Bone Marrow Transplant Unit, East Wing, Providence, Rhode Island 02908-4735, USA. BERZ@sbcglobal.net
McCormack
Elise M
EM
Winer
Eric S
ES
Colvin
Gerald A
GA
Quesenberry
Peter J
PJ
D003114:Colony-Forming Units Assay; D015925:Cryopreservation / Q000379:methods / Q000592:standards; D005615:Freezing; D006412:Hematopoietic Stem Cells; D006801:Humans; D017053:Infection Control