Beta vulgaris L. suspension cultures permeabilized with triton X-100 retain cell viability and betacyanines production ability: a digital image analysis study.
The influence of Triton X-100 on Beta vulgaris L. permeabilized cell culture viability, regrowth, and ability to produce betacyanines was evaluated in this study. A non-destructive method based on the analysis of images in the RGB (red, green, blue) system was developed to estimate betacyanines content. A treatment for 15 min with 0.7 mM Triton X-100 induced the release of 30% of betacyanines without loss of cell viability (>or=70%). After this permeabilization treatment, B. vulgaris cultures regrew normally, reaching a maximum biomass concentration of 48% higher than non-permeabilized cultures after 14 days of culture. Also, maximum betacyanines concentration was only 25% lower than that of non-permeabilized cultures.
Trejo-Tapia
Gabriela
G
Departamento de Biotecnología, Centro de Desarrollo de Productos Bióticos del IPN, P.O. Box 24, 62730 Yautepec, Morelos, Mexico.
Cuevas-Celis
Jonathan
J
Salcedo-Morales
Guadalupe
G
Trejo-Espino
José Luis
JL
Arenas-Ocampo
Martha L
ML
Jiménez-Aparicio
Antonio
A
D027461:Beta vulgaris / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D050859:Betacyanins / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D002470:Cell Survival; D003124:Colorimetry / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods*; D017830:Octoxynol / Q000008:administration & dosage*; D010539:Permeability / Q000187:drug effects; D012815:Signal Processing, Computer-Assisted; D015398:Signal Transduction / Q000187:drug effects
D050859:Betacyanins; D017830:Octoxynol