title: Effects of dietary salt load and salt depletion on the course of hypertension and angiotensin II levels in male and female heterozygous Ren-2 transgenic rats.
abstract: BACKGROUND
  In the present study we evaluated plasma and kidney angiotensin II (ANG II) levels in female and male Ren-2 transgenic rats (TGR) in comparison to age-matched female and male normotensive Hannover Sprague-Dawley rats.
  
  
  METHODS
  The rats were maintained on a normal sodium (NS) diet (0.6% NaCl) or fed a high sodium (HS) diet (2% NaCl) for 4 days or were sodium depleted by administration of 40 mg furosemide per liter drinking water overnight followed by 3 days of low sodium diet (0.01% NaCl) (LS + F). ANG II levels were determined by radioimmunoassay.
  
  
  RESULTS
  Female TGR at the age of 38 days were already hypertensive and had developed cardiac hypertrophy, whereas male TGR at this age still exhibited a normotensive phenotype. HS diet increased the blood pressure (BP) but did not alter the ANG II levels in TGR at any age. LS + F decreased the BP without significant change in ANG II concentrations in TGR. Female TGR responded to salt loading and salt depletion by more pronounced changes in BP than male TGR.
  
  
  CONCLUSIONS
  Female TGR develop hypertension more rapidly and the salt-sensitive component of hypertension is more pronounced in female than in male TGR.
authors.affiliation: Department of Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Husková
authors.forename: Herbert
authors.initials: H
authors.lastname: Kramer
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Vanourková
authors.forename: Monika
authors.initials: M
authors.lastname: Thumová
authors.forename: Jan
authors.initials: J
authors.lastname: Malý
authors.forename: Martin
authors.initials: M
authors.lastname: Opocenský
authors.forename: Petra
authors.initials: P
authors.lastname: Skaroupková
authors.forename: Alexander
authors.initials: A
authors.lastname: Kolský
authors.forename: Zdena
authors.initials: Z
authors.lastname: Vernerová
authors.forename: Ludek
authors.initials: L
authors.lastname: Cervenka
mesh_terms: D000804:Angiotensin II / Q000097:blood* / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D030801:Animals, Genetically Modified; D001794:Blood Pressure; D006332:Cardiomegaly / Q000097:blood / Q000178:diet therapy; D005260:Female; D006579:Heterozygote; D006977:Hypertension, Renal / Q000097:blood / Q000178:diet therapy*; D007668:Kidney / Q000098:blood supply / Q000503:physiopathology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012083:Renin / Q000235:genetics* / Q000378:metabolism; D012084:Renin-Angiotensin System / Q000502:physiology; D012727:Sex Characteristics; D017673:Sodium Chloride, Dietary / Q000627:therapeutic use*
chemical_list: D017673:Sodium Chloride, Dietary; D000804:Angiotensin II; D012083:Renin