Olivibacter sitiensis gen. nov., sp. nov., isolated from alkaline olive-oil mill wastes in the region of Sitia, Crete.
A novel, Gram-negative, non-motile, non-sporulating, rod-shaped bacterium isolated from a viscous two-phase olive-oil mill waste ('alpeorujo') is described. The strain, designated AW-6T, is an obligate aerobe, forming irregular, pigmented creamy white colonies. The pH and temperature ranges for growth were pH 5-8 and 5-45 degrees C, with optimal pH and temperature for growth of pH 6-7 and 28-32 degrees C, respectively. Strain AW-6T was chemo-organotrophic and utilized mostly D+ -glucose, protocatechuate and D+ -xylose, followed by L-cysteine, D- -fructose, D+ -galactose, L-histidine, lactose, sorbitol and sucrose. Menaquinone-7 was detected in the respiratory chain of strain AW-6T. The major fatty acids of strain AW-6T were C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH, iso-C(15 : 0), iso-C(17 : 0) 3-OH and C(16 : 0). The closest phylogenetic relative of strain AW-6T was clone BIti35 (89.7 % 16S rRNA gene sequence similarity), while Sphingobacterium thalpophilum DSM 11723T was the closest recognized relative within the Sphingobacteriaceae (88.2 % similarity). Strain AW-6T showed a low level of DNA-DNA relatedness to S. thalpophilum DSM 11723T (33.8-37.0 %). The DNA G+C content of strain AW-6T was 45.6 mol%. Physiological and chemotaxonomic data further confirmed the distinctiveness of strain AW-6T from members of the genera Sphingobacterium and Pedobacter. Thus, strain AW-6T is considered to represent a novel species of a new genus within the family Sphingobacteriaceae, for which the name Olivibacter sitiensis gen. nov., sp. nov. is proposed. The type strain is AW-6T =DSM 17696T=CECT 7133T).
Ntougias
Spyridon
S
National Agricultural Research Foundation, Institute of Kalamata, Lakonikis 87, 24100 Kalamata, Greece.
Fasseas
Constantinos
C
Agricultural University of Athens, Department of Agricultural Biotechnology, Electron Microscopy Laboratory, Iera Odos 75, 11855 Athens, Greece.
Zervakis
Georgios I
GI
National Agricultural Research Foundation, Institute of Kalamata, Lakonikis 87, 24100 Kalamata, Greece.
D000332:Aerobiosis; D000596:Amino Acids / Q000378:metabolism; D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D001482:Base Composition; D050260:Carbohydrate Metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry; D020459:Genes, rRNA; D006115:Greece; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D008969:Molecular Sequence Data; D009068:Movement; D000069463:Olive Oil; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D010938:Plant Oils; D011809:Quinones / Q000032:analysis / Q000737:chemistry; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013171:Spores, Bacterial; D013696:Temperature
D000596:Amino Acids; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D007220:Industrial Waste; D000069463:Olive Oil; D010860:Pigments, Biological; D010938:Plant Oils; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S