Susceptibility of CCR5-deficient mice to genital herpes simplex virus type 2 is linked to NK cell mobilization.
Following genital herpes simplex virus type 2 (HSV-2) exposure, NK cells and T cells are mobilized to sites of infection to control viral replication and spread. The present investigation sought to determine the role of the chemokine receptor CCR5 in this process. Mice deficient in CCR5 (CCR5-/-) displayed a significant reduction in cumulative survival following infection in comparison to wild-type, HSV-2-infected controls. Associated with decreased resistance to viral infection, CCR5-/- mice yielded significantly more virus and expressed higher levels of tumor necrosis factor alpha, CXCL1, CCL2, CCL3, and CCL5 in the vagina, spinal cord, and/or brain stem than did wild-type mice. Whereas there was no difference in absolute number of leukocytes (CD45high), CD4 T cells, or CD8 T cells residing in the draining lymph nodes, spleen, spinal cord, or brain stem comparing HSV-2-infected wild-type to CCR5-/- mice prior to or after infection, there were significantly more NK cells (NK1.1+ CD3-) residing in the brain stem and spleen of infected wild-type mice. Functionally, NK activity from cells isolated from the brain stem of HSV-2-infected wild-type mice was greater than that from HSV-2-infected CCR5-/- mice. In addition, antibody-mediated depletion of NK cells resulted in an increase in HSV-2 levels in the vaginal, spinal cord, and brain stem tissue of wild-type but not CCR5-/- mice. Collectively, the absence of CCR5 expression significantly impacts the ability of the host to control genital HSV-2 infection, inflammation, and spread associated with a specific reduction in NK cell expansion, infiltration, and activity in the nervous system.
Thapa
Manoj
M
Department of Microbiology, DMEI #415, The University of Oklahoma Health Sciences Center, 608 Stanton L. Young Blvd., Oklahoma City, OK 73104, USA.
Kuziel
William A
WA
Carr
Daniel J J
DJ
D000818:Animals; D001933:Brain Stem / Q000166:cytology / Q000276:immunology / Q000821:virology; D018925:Chemokines / Q000096:biosynthesis; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression; D053804:Granzymes / Q000096:biosynthesis; D006558:Herpes Genitalis / Q000276:immunology*; D018258:Herpesvirus 2, Human / Q000276:immunology*; D007113:Immunity, Innate; D007694:Killer Cells, Natural / Q000276:immunology*; D047589:Leukocyte Reduction Procedures; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D052899:Pore Forming Cytotoxic Proteins / Q000096:biosynthesis; D019713:Receptors, CCR5 / Q000172:deficiency / Q000276:immunology*; D013116:Spinal Cord / Q000276:immunology / Q000821:virology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D016019:Survival Analysis; D014621:Vagina / Q000276:immunology / Q000821:virology; D014779:Virus Replication; D014780:Viruses
D018925:Chemokines; D052899:Pore Forming Cytotoxic Proteins; D019713:Receptors, CCR5; C483886:perforin, mouse; D053804:Granzymes