Ire1 regulated XBP1 mRNA splicing is essential for the unfolded protein response (UPR) in Drosophila melanogaster.
The ability of cells to survive and recover from deteriorating effects of endoplasmic reticulum (ER) stress relies on the unfolded protein response (UPR). The signaling pathway of Ire1p mediate mRNA splicing plays diverge role in UPR response in different organisms from yeast to mammals. Here, we report that Ire1p mediated XBP1 mRNA splicing mechanism is extremely conserved and exerts a critical role for modulating Xbp1 protein synthesis in Drosophila melanogaster. This system is operative in Drosophila S2 cells as a prominent mechanism to mediate transcriptional activation of UPR responsive genes during ER stress.
Plongthongkum
Nongluk
N
The Institute of Molecular Biology and Genetics, Mahidol University, Salaya, Nakhonpathom 73170, Thailand.
Kullawong
Niwed
N
Panyim
Sakol
S
Tirasophon
Witoon
W
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D004331:Drosophila melanogaster; D004721:Endoplasmic Reticulum / Q000378:metabolism; D004722:Endoribonucleases / Q000502:physiology*; D018832:Molecular Chaperones / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology; D012326:RNA Splicing; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D018832:Molecular Chaperones; D029701:Saccharomyces cerevisiae Proteins; C517798:Xbp1 protein, Drosophila; C488472:unfolding protein, S cerevisiae; D004722:Endoribonucleases; C511918:inositol requiring enzyme-1, Drosophila