Influence of plant matrix on microwave-assisted extraction process. The case of diosgenin extracted from fenugreek (Trigonella foenum-graecum L.).
A focused microwave-assisted extraction method was developed for the extraction of diosgenin from fenugreek (Trigonella foenum-graecum) seeds, air-dried and fresh leaves and air-dried roots. Several experimental parameters were studied, including extraction time, microwave power applied and percentage of 2-propanol in the extraction mixture as well as their interactions, in order to optimize the extraction efficiency. The two latter parameters were found to be the most important. Response surface modelling was used to predict the extraction yield of diosgenin in selected matrices. The analysis of diosgenin in crude extracts was carried out by capillary gas chromatography-mass spectrometry (GC-MS).
Kaufmann
Béatrice
B
Laboratory of Pharmaceutical Analytical Chemistry, School of Pharmaceutical Sciences EPGL, University of Geneva, 20 bd d'Yvoy, CH-1211 Geneva 4, Switzerland.
Rudaz
Serge
S
Cherkaoui
Samir
S
Veuthey
Jean-Luc
JL
Christen
Philippe
P
D000465:Algorithms; D004144:Diosgenin / Q000737:chemistry* / Q000302:isolation & purification; D008401:Gas Chromatography-Mass Spectrometry; D008872:Microwaves; D010936:Plant Extracts / Q000032:analysis; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry; D029910:Trigonella / Q000737:chemistry*
D010936:Plant Extracts; D004144:Diosgenin