title: C/EBPbeta participates in regulating transcription of the p53 gene in response to mitogen stimulation.
abstract: The tightly regulated expression of p53 contributes to genomic stability, and transcription of the p53 gene is induced prior to cells entering S phase, possibly as a mechanism to ensure a rapid p53 response in the event of DNA damage. We have previously described the cloning of an additional 1000 bp of upstream p53 sequences that we have demonstrated play a role in the regulated expression of p53. As described in an earlier report, we preliminarily identified that a member of the CAAT/enhancer-binding protein (C/EPB) family of transcription factors may play a role in regulating p53. Here we have demonstrated that a particular C/EBPbeta isoform, C/EBPbeta-2, efficiently binds to the p53 promoter and induces its expression in a fashion that reflects the pattern of p53 expression seen as cells are induced to enter S phase and is absent from cells that are defective in proper p53 regulation. We conclude from these findings that C/EBPbeta-2 plays a central role in the regulating of p53 transcription during the transition into S phase.
authors.affiliation: Department of Biological Sciences, University of South Carolina, Columbia, South Carolina 29208, USA.
authors.forename: Kristy
authors.initials: K
authors.lastname: Boggs
authors.forename: David
authors.initials: D
authors.lastname: Reisman
mesh_terms: D000818:Animals; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000378:metabolism / Q000502:physiology*; D002453:Cell Cycle; D003001:Cloning, Molecular; D005347:Fibroblasts / Q000378:metabolism; D017353:Gene Deletion; D005786:Gene Expression Regulation; D016158:Genes, p53; D051379:Mice; D008822:Mice, Transgenic; D008934:Mitogens / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D016196:S Phase; D014158:Transcription, Genetic; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D022782:CCAAT-Enhancer-Binding Protein-beta; D008934:Mitogens; D016159:Tumor Suppressor Protein p53