title: Direct polymerase chain reaction amplification of formalin-fixed, paraffin-wax-embedded tissue after automated sequential laser microdissection and pressure catapulting.
abstract: A robust method to facilitate rapid laser microdissection and pressure catapulting (LMPC) coupled with direct polymerase chain reaction (dPCR) to eliminate the need for extraction of DNA before a PCR-based assay is described. This sequential LMPC-dPCR method is rapid and decreases the number of processing steps, reducing the chance of tissue loss and contamination.
authors.affiliation: Cancer Biology Proteomics Group, Postgraduate Medical Institute of the University of Hull in association with the Hull-York Medical School, University of Hull, Hull, UK.
authors.forename: S L
authors.initials: SL
authors.lastname: O'Kane
authors.forename: V
authors.initials: V
authors.lastname: Garimella
authors.forename: N
authors.initials: N
authors.lastname: Sivarajasingham
authors.forename: P J
authors.initials: PJ
authors.lastname: Drew
authors.forename: L
authors.initials: L
authors.lastname: Cawkwell
mesh_terms: D005557:Formaldehyde; D006801:Humans; D007834:Lasers; D042282:Microdissection / Q000379:methods*; D018895:Microsatellite Repeats; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction / Q000379:methods*; D016707:Tissue Fixation / Q000379:methods
chemical_list: D005557:Formaldehyde