Isolation of a gene that is involved in Campylobacter jejuni 81116 cytotoxin activation.
Cytotoxin fractions were isolated from Campylobacter jejuni 81116 and semi-purified by size-exclusion liquid chromatography. The fraction showing the strongest toxicity was injected into mice to produce antiserum. The antiserum was used to screen a C. jejuni 81116 cosmid library. Nine genes were identified in overlapping cosmid inserts that induced reactivity with the antiserum. One of these genes showed high similarity to a periplasmic protein of unknown function and its isogenic mutant showed decreased toxicity compared to the C. jejuni 81116 wild type. This gene contains a Gram-negative bacterial RTX toxin-activating protein C signature, which suggests it may play a role in C. jejuni 81116 cytotoxin activation.
Liu
Kaiyan
K
Landguard Development Facility, Orica Australia Pty Ltd. Bundoora, Victoria, Australia.
Fry
Benjamin N
BN
Coloe
Peter J
PJ
D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western / Q000379:methods; D016466:CHO Cells; D002169:Campylobacter Infections / Q000382:microbiology; D016123:Campylobacter jejuni / Q000235:genetics* / Q000276:immunology; D003360:Cosmids / Q000235:genetics*; D006224:Cricetinae; D003412:Cricetulus; D003603:Cytotoxins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009500:Neutralization Tests / Q000379:methods; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction / Q000379:methods; D015183:Restriction Mapping
D003603:Cytotoxins; D004269:DNA, Bacterial