title: Selective cell proliferation can be controlled with CPC particle coatings.
abstract: To develop implantable, engineered, cartilage constructs supported by a scaffold, techniques to encourage rapid tissue growth into, and on the scaffold are essential. Preliminary studies indicated that human endothelial cells proliferated at different rates on different calcium phosphate ceramic (CPC) particles. Judicious selection of particles may encourage specific cell proliferation, leading to an ordered growth of tissues for angiogenesis, osteogenesis, and chondrogenesis. The goal of this study was to identify CPC surfaces that encourage bone and vascular cell growth, and other surfaces that support chondrocyte growth while inhibiting proliferation of vascular cells. Differences in bone and vascular cell proliferation were observed when using epoxy without embedded CPCs to encourage bone cells, and when three CPCs were tested, which encouraged vascular cell proliferation. One of these (CPC 7) also substantially depressed cartilage cell proliferation. Only one small-diameter crystalline CPC (CPC 2) supported rapid chondrocyte proliferation, and maintained the cartilage cell phenotype.
authors.affiliation: Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, University of Arizona, Tucson, Arizona, USA. szivek@u.arizona.edu
authors.forename: J A
authors.initials: JA
authors.lastname: Szivek
authors.forename: D S
authors.initials: DS
authors.lastname: Margolis
authors.forename: A B
authors.initials: AB
authors.lastname: Schnepp
authors.forename: W A
authors.initials: WA
authors.lastname: Grana
authors.forename: S K
authors.initials: SK
authors.lastname: Williams
mesh_terms: D001842:Bone and Bones / Q000166:cytology* / Q000187:drug effects; D002130:Calcium Phosphates / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002516:Ceramics / Q000378:metabolism*; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D011509:Proteoglycans / Q000378:metabolism; D013499:Surface Properties; D014961:X-Ray Diffraction
chemical_list: D002130:Calcium Phosphates; D011509:Proteoglycans; C020243:calcium phosphate