Quantitation of Armillarisin A in human plasma by liquid chromatography-electrospray tandem mass spectrometry.
A rapid and sensitive LC-MS/MS method for quantifying Armillarisin A in human plasma after a single oral dose (40 mg) has been developed and validated. Sample preparation used liquid-liquid extraction with a mixture of diethyl ether-dichloromethane (60:40, v/v) in an acidic environment. The retention times of Armillarisin A and the internal standard, probenecid, were 1.63 and 1.78 min, respectively. The calibration curve was linear over the range 0.15-50 ng/mL with a limit of quantitation of 0.15 ng/mL. The coefficient of variation as a measure of intra- and inter-day precision was <9.3% and the accuracy was in the range 92.5-108.0%. The Armillarisin A concentration-time profile in human plasma was determined after an oral dose of a 40 mg tablet.
Wang
Yan
Y
Research Center for Drug Metabolism, Jilin University, Changchun 130023, China.
Wang
Yingwu
Y
Li
Pengfei
P
Tang
Yunbiao
Y
Fawcett
J Paul
JP
Gu
Jingkai
J
D000284:Administration, Oral; D001578:Benzopyrans / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D003374:Coumarins / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D004986:Ether / Q000737:chemistry; D006801:Humans; D008752:Methylene Chloride / Q000737:chemistry; D015394:Molecular Structure; D011339:Probenecid / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013607:Tablets; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors
D001578:Benzopyrans; D003374:Coumarins; D013607:Tablets; D004986:Ether; D008752:Methylene Chloride; C023435:armillarisin A; D011339:Probenecid