Collagenase-2 (MMP-8) and matrilysin-2 (MMP-26) expression in human wounds of different etiologies.
Wound healing involves highly controlled events including reepithelialization, neoangiogenesis, and reformation of the stromal compartment. Matrix metalloproteinases (MMPs) are a family of neutral zinc-dependent endopeptidases known to be essential for the wound-healing process. MMP-8 (collagenase-2) is a neutrophil-derived highly effective type I collagenase, recently indicated to be important for acute wound healing. MMP-26 is a more recent and less well-studied member of the MMP family. Our aim was to study the expression of MMP-8 and MMP-26 in human cutaneous wound repair and chronic wounds using histological methods and cell culture. MMP-8 expression was associated with epithelial cells, neutrophils, and other inflammatory cells in chronic human wounds. MMP-26 was prominently expressed in the extracellular compartment of most chronic wounds in close vicinity to the basement membrane area. MMP-26 was also expressed in acute day 1 wounds with declining expression thereafter. In vitro wound experiments showed that both MMP-8 and MMP-26 were expressed by migrating human mucosal keratinocytes. Inhibiting MMP-26 resulted in aberrant keratinocyte migration and proliferation. We conclude that MMP-8 and MMP-26 are differentially expressed in acute and chronic wounds.
Pirilä
Emma
E
Institute of Dentistry, University of Oulu, Oulu, Finland. emma.pirila@oulu.fi
Korpi
Jarkko T
JT
Korkiamäki
Timo
T
Jahkola
Tiina
T
Gutierrez-Fernandez
Ana
A
Lopez-Otin
Carlos
C
Saarialho-Kere
Ulpu
U
Salo
Tuula
T
Sorsa
Timo
T
D000208:Acute Disease; D000368:Aged; D000369:Aged, 80 and over; D002908:Chronic Disease; D048909:Diabetes Complications / Q000150:complications; D005260:Female; D006801:Humans; D008297:Male; D020784:Matrix Metalloproteinase 8 / Q000378:metabolism*; D053505:Matrix Metalloproteinases, Secreted / Q000378:metabolism*; D008875:Middle Aged; D012883:Skin Ulcer / Q000209:etiology* / Q000378:metabolism*; D014647:Varicose Ulcer / Q000378:metabolism*; D014657:Vasculitis / Q000150:complications; D014945:Wound Healing / Q000502:physiology
C410599:MMP26 protein, human; D053505:Matrix Metalloproteinases, Secreted; C488762:MMP8 protein, human; D020784:Matrix Metalloproteinase 8