title: Analysis on the factors affecting start-up intensity in the upstream sequence of phycocyanin beta subunit gene from Arthrospira platensis by site-directed mutagenesis.
abstract: Six promoters in the 419 bp upstream sequence of the phycocyanin beta subunit gene of Arthrospira platensis FACHB341 have been previously cloned. Site-directed mutagenesis has now been used to introduce mutations in the -10 and -35 boxes of promoter 3, -10 box of promoter 4, and -35 box of promoter 6. The expression level of green fluorescent protein gene was measured by flow cytometry. Results showed that the effects of site-directed mutagenesis in different promoters were dissimilar: some increased and some declined.
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao, PR China.
authors.forename: Nan
authors.initials: N
authors.lastname: Guo
authors.forename: Xuecheng
authors.initials: X
authors.lastname: Zhang
authors.forename: Yongzhong
authors.initials: Y
authors.lastname: Lu
authors.forename: Xiaojin
authors.initials: X
authors.lastname: Song
mesh_terms: D001483:Base Sequence; D000458:Cyanobacteria / Q000235:genetics*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D010798:Phycocyanin / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D015202:Protein Engineering / Q000379:methods*; D021122:Protein Subunits / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*
chemical_list: D021122:Protein Subunits; D010798:Phycocyanin