Phylogenetic analysis of two putative Nosema isolates from Cruciferous Lepidopteran pests in Taiwan.
In this study, a new microsporidian, PX2, was isolated from the diamondback moth, Plutella xylostella, and then compared with another isolate (PX1), and with Nosema spodopterae and N. bombycis. Sequence data showed that the rRNA gene organizations of PX1 and PX2 exhibited a typical Nosema-specific organization: 5'-LSUrRNA (large subunit ribosomal RNA)-ITS (internal transcribed spacer)-SSUrRNA-IGS (intergenic spacer)-5S-3'. Phylogenetic analysis (maximum likelihood, neighbor joining, maximum parsimony, and Bayesian analysis) of the LSUrRNA and SSUrRNA gene sequences, and the sequences of the alpha-tubulin, beta-tubulin, and RPB1 (DNA dependent RNA polymerase II largest subunit) genes found that PX1 was closer to N. bombycis and N. spodopterae than to PX2. Comparison of the identities of the rRNA domains and of the other three genes showed a high divergence in the sequences of the rRNA spacer regions (ITS and IGS). This is consistent with the hypothesis that PX2, if not PX1, might represent a new Nosema species.
Ku
Chin-Tai
CT
Department of Entomology, National Taiwan University, Taipei 106, Taiwan.
Wang
Chih-Yuan
CY
Tsai
Yi-Chun
YC
Tzeng
Ching-Chou
CC
Wang
Chung-Hsiung
CH
D000818:Animals; D019607:Brassicaceae; D004271:DNA, Fungal / Q000235:genetics; D007915:Lepidoptera / Q000382:microbiology*; D016881:Microsporidiosis / Q000235:genetics; D016818:Nosema / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity*; D010802:Phylogeny; D012331:RNA, Fungal / Q000235:genetics; D013624:Taiwan
D004271:DNA, Fungal; D012331:RNA, Fungal