[The N-terminal pro-brain natriuretic peptide (NT-proBNP) assay with the Stratus CS analyzer].
BACKGROUND
NT-proBNP is an efficient biomarker for the evaluation, management and prognosis of patients with heart failure.


METHODS
We evaluated the analytical performance of the NT-proBNP immunoenzymatic assay with the Stratus CS semi-automated analyzer in two hospital laboratories. The characteristics assessed included imprecision, functional sensitivity, linearity/recovery, interferences study, high-dose hook effect and a comparison of Acute Care(TM) pPBNP (on Stratus)CS) versus PBNP (on Dimension HM) results on patient heparinized plasma samples.


RESULTS
Total imprecision reached < 5% coefficient of variation at NT-proBNP concentrations of 186-19,649 ng/L; recovery values for diluted samples were between 89.0 and 110.0 %; functional sensitivity reached 21 ng/L; there was no high-dose hook effect at concentrations up to 400,000 ng/L; hemaoglobin affected negatively but <10% the NT-proBNP assay, while bilirubin and triglycerides did not affected it more than 5%; Stratus CS results were strongly correlated with Dimension HM results (R(2)=1,00).


CONCLUSIONS
The Stratus CS Acute Care pPBNP assay demonstrated excellent analytical performance which agreed with the Dimension HM PBNP assay. This analyzer is therefore suitable for use by low NT-proBNP test volume laboratories, and also by Emergency departments and Intensive care units.
Chenevier-Gobeaux
C
C
Laboratoire de Biochimie A, Hôpital Cochin, Assistance Publique - Hôpitaux de Paris (AP-HP), Paris. camille.gobeaux@cch.ap-hop-paris.fr
Guillot
D
D
Ursulet
J
J
Paul
J-L
JL
Ekindjian
O G
OG
Desmoulins
D
D
Benoit
M-O
MO
D015415:Biomarkers / Q000097:blood; D006333:Heart Failure / Q000097:blood*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D016014:Linear Models; D020097:Natriuretic Peptide, Brain / Q000097:blood*; D010446:Peptide Fragments / Q000097:blood*; D015203:Reproducibility of Results
D015415:Biomarkers; D010446:Peptide Fragments; C109794:pro-brain natriuretic peptide (1-76); D020097:Natriuretic Peptide, Brain