title: Sherlock Holmes and the proteome--a detective story.
abstract: The performance of a hexapeptide ligand library in capturing the 'hidden proteome' is illustrated and evaluated. This library, insolubilized on an organic polymer and available under the trade name 'Equalizer Bead Technology', acts by capturing all components of a given proteome, by concentrating rare and very rare proteins, and simultaneously diluting the abundant ones. This results in a proteome of 'normalized' relative abundances, amenable to analysis by MS and any other analytical tool. Examples are given of analysis of human urine and serum, as well as cell and tissue lysates, such as Escherichia coli and Saccharomyces cerevisiae extracts. Another important application is impurity tracking and polishing of recombinant DNA products, especially biopharmaceuticals meant for human consumption.
authors.affiliation: Department of Chemistry Materials and Chemical Engineering Giulio Natta, Polytechnic of Milano, Milan, Italy. piergiorgio.righetti@polimi.it
authors.forename: Pier Giorgio
authors.initials: PG
authors.lastname: Righetti
authors.forename: Egisto
authors.initials: E
authors.lastname: Boschetti
mesh_terms: D015415:Biomarkers / Q000737:chemistry; D001798:Blood Proteins / Q000032:analysis / Q000302:isolation & purification; D020650:Combinatorial Chemistry Techniques; D004274:DNA, Recombinant / Q000737:chemistry; D029968:Escherichia coli Proteins / Q000032:analysis / Q000302:isolation & purification; D006801:Humans; D008024:Ligands; D013058:Mass Spectrometry; D019151:Peptide Library; D020543:Proteome / Q000032:analysis* / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods*; D029701:Saccharomyces cerevisiae Proteins / Q000032:analysis / Q000302:isolation & purification; D014556:Urine / Q000737:chemistry
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D004274:DNA, Recombinant; D029968:Escherichia coli Proteins; D008024:Ligands; D019151:Peptide Library; D020543:Proteome; D029701:Saccharomyces cerevisiae Proteins