Upregulation of transglutaminase in the goldfish retina during optic nerve regeneration.
To elucidate the molecular involvement of transglutaminase (TG) in central nervous system (CNS) regeneration, we cloned a full-length cDNA for neural TG (TG(N)) from axotomized goldfish retinas and produced a recombinant TG(N) protein from this cDNA. The levels of TG(N) mRNA and protein were increased at 10-30 days after optic nerve transection, and this increase in TG(N) was only localized in the ganglion cells in goldfish retinas. In retinal explant cultures, the recombinant TG(N) protein induced a drastic enhancement of neurite outgrowth, while TG(N)-specific RNAi significantly suppressed this neurite outgrowth. Taken together, these data strongly indicate that TG(N) is a key regulatory molecule for CNS regeneration.
Sugitani
Kayo
K
Department of Molecular Neurobiology, Graduate School of Medicine, Kanazawa University, 13-1 Takara-machi, Kanazawa 920-8640, Japan.
Matsukawa
Toru
T
Maeda
Ari
A
Kato
Satoru
S
D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D006054:Goldfish; D009416:Nerve Regeneration; D016501:Neurites / Q000378:metabolism*; D009474:Neurons / Q000201:enzymology* / Q000378:metabolism; D009900:Optic Nerve / Q000473:pathology*; D020221:Optic Nerve Injuries / Q000473:pathology; D034622:RNA Interference; D011994:Recombinant Proteins / Q000737:chemistry; D012160:Retina / Q000201:enzymology* / Q000378:metabolism*; D011503:Transglutaminases / Q000235:genetics*; D015854:Up-Regulation
D018076:DNA, Complementary; D011994:Recombinant Proteins; D011503:Transglutaminases