Production and characterization of monoclonal antibodies against a major membrane protein of Mycobacterium avium subsp. paratuberculosis.
The Mycobacterium avium subsp. paratuberculosis 35-kDa major membrane protein (MMP) encoded by MAP2121c is an important membrane antigen recognized in cattle with Johne's disease. In this study, purified recombinant MMP was used to produce two stable monoclonal antibodies, termed 8G2 and 13E1, which were characterized by immunoblotting, epitope mapping, and immunofluorescence microscopy.
Bannantine
John P
JP
National Animal Disease Center, ARS-USDA, 2300 North Dayton Ave., Ames, IA 50010, USA. jbannant@nadc.ars.usda.gov
Radosevich
Thomas J
TJ
Stabel
Judith R
JR
Berger
Sven
S
Griffin
J Frank T
JF
Paustian
Michael L
ML
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology*; D005260:Female; D008565:Membrane Proteins / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology*; D010283:Paratuberculosis / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000276:immunology*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins