title: Enzymatic formation of the sarpagan-bridge: a key step in the biosynthesis of sarpagine- and ajmaline-type alkaloids.
abstract: The glucoalkaloid strictosidine has been converted under cell-free conditions into 10-deoxysarpagine (= normacusine B) in the presence of a crude soluble enzyme extract and microsomal protein isolated from cell suspensions of Rauwolfia serpentina. The enzymatic formation of this alkaloid bearing the C-5/C-16 bond (sarpagan-bridge), which is characteristic for all sarpagine- and ajmaline-type alkaloids, is dependent on NADPH and oxygen. Inhibition studies indicate that for the synthesis of 10-deoxysarpagine a cytochrome P450 dependent monoxygenase is necessary.
authors.affiliation: Lehrstuhl für Pharmazeutische Biologie der Johannes Gutenberg-Universität, Mainz, Institut für Pharmazie, Staudinger Weg 5, D-55099 Mainz, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Schmidt
authors.forename: J
authors.initials: J
authors.lastname: Stöckigt