title: Development of a new E. coli strain to detect oxidative mutation and its application to the fungicide o-phenylphenol and its metabolites.
abstract: Oxidative mutation is mainly induced by reactive oxygen species (ROS), such as the superoxide anion radical (O(2)(-)) and hydrogen peroxide (H(2)O(2)). However, in Escherichia coli (E. coli), ROS are eliminated by enzymes such as superoxide dismutase and catalase, which are coded by sodAB and katEG genes. In this study, to detect mutagens that induce oxidative mutation, a mutant (WP2katEGsodAB) with katEG and sodAB deleted was constructed by gene manipulation of E. coli WP2. H(2)O(2) and menadione sodium bisulfite generated mutation in WP2katEGsodAB but not in WP2. o-Phenylphenol (OPP) and its metabolites (phenylhydroquinone (PHQ) and phenyl-1,4-benzoquinone (PBQ)), which had been shown to be negative in the Ames test but reported to be carcinogenic, induced mutation in WP2katEGsodAB but not in WP2. These results suggest that the new assay may be useful for the detection of oxidative mutagens.
authors.affiliation: Urban Environmental Engineering, Kyoto University, Kyoto 606-8540, Japan. tani@risk.env.kyoto-u.ac.jp
authors.forename: Shusuke
authors.initials: S
authors.lastname: Tani
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Yonezawa
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Morisawa
authors.forename: Hajime
authors.initials: H
authors.lastname: Nishioka
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D016227:Benzoquinones / Q000633:toxicity; D001713:Biphenyl Compounds / Q000378:metabolism / Q000633:toxicity*; D002374:Catalase / Q000235:genetics; D004249:DNA Damage; D024881:Drug Resistance, Bacterial / Q000187:drug effects / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics; D005659:Fungicides, Industrial / Q000378:metabolism / Q000633:toxicity*; D006861:Hydrogen Peroxide / Q000633:toxicity; D006873:Hydroquinones / Q000633:toxicity; D008769:Methylnitronitrosoguanidine / Q000633:toxicity; D050296:Microbial Viability / Q000187:drug effects; D009154:Mutation; D030781:Organisms, Genetically Modified; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000235:genetics; D024483:Vitamin K 3 / Q000633:toxicity
chemical_list: D001426:Bacterial Proteins; D016227:Benzoquinones; D001713:Biphenyl Compounds; D029968:Escherichia coli Proteins; D005659:Fungicides, Industrial; D006873:Hydroquinones; D008769:Methylnitronitrosoguanidine; C029322:phenylbenzoquinone; D024483:Vitamin K 3; D006861:Hydrogen Peroxide; C004369:2-phenylphenol; C102664:hydroperoxidase II; D002374:Catalase; C051253:katG protein, E coli; C095500:SodA protein, Bacteria; C087504:SodB protein, Bacteria; D013482:Superoxide Dismutase