Oviduct-specific expression of two therapeutic proteins in transgenic hens.
Recent advances in avian transgenesis have led to the possibility of utilizing the laying hen as a production platform for the large-scale synthesis of pharmaceutical proteins. Ovalbumin constitutes more than half of the protein in the white of a laid egg, and expression of the ovalbumin gene is restricted to the tubular gland cells of the oviduct. Here we describe the use of lentiviral vectors to deliver transgene constructs comprising regulatory sequences from the ovalbumin gene designed to direct synthesis of associated therapeutic proteins to the oviduct. We report the generation of transgenic hens that synthesize functional recombinant pharmaceutical protein in a tightly regulated tissue-specific manner, without any evidence of transgene silencing after germ-line transmission.
Lillico
S G
SG
Roslin Institute, Roslin Biocentre, Midlothian EH25 9PS, United Kingdom.
Sherman
A
A
McGrew
M J
MJ
Robertson
C D
CD
Smith
J
J
Haslam
C
C
Barnard
P
P
Radcliffe
P A
PA
Mitrophanous
K A
KA
Elliot
E A
EA
Sang
H M
HM
D000818:Animals; D030801:Animals, Genetically Modified; D002645:Chickens / Q000235:genetics* / Q000378:metabolism; D004527:Egg Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000627:therapeutic use; D004529:Egg White / Q000737:chemistry; D005260:Female; D005822:Genetic Vectors; D006801:Humans; D004860:Infectious Anemia Virus, Equine / Q000235:genetics; D000068556:Interferon beta-1a; D016899:Interferon-beta / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology; D008297:Male; D051379:Mice; D010057:Oviducts / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism
D004527:Egg Proteins; D011994:Recombinant Proteins; D016899:Interferon-beta; D000068556:Interferon beta-1a