title: Altered gene expression in Choristoneura fumiferana and Manduca sexta in response to sublethal intoxication by Bacillus thuringiensis Cry1Ab toxin.
abstract: In order to understand how lepidopteran insects react physiologically to Bacillus thuringiensis crystal toxin ingestion, transcriptional profiling of Choristoneura fumiferana larvae exposed to sublethal doses of Cry1Ab protoxin were monitored using a C. fumiferana-specific cDNA microarray derived from a protoxin-specific subtractive library. Differential gene expression occurred primarily between 2 and 5 h postingestion. Metabolic enzymes such as lipases and proteases were generally repressed, whereas genes involved in detoxification, immune system regulation or general stress response were upregulated. A similar protoxin-specific transcriptional pattern was also observed with Manduca sexta larvae, using three upregulated genes (serpin, cytochrome P450 and carboxyl/cholinesterase) and one downregulated gene (beta-glucosidase), suggesting that a susceptible larval response to Cry toxin exposure might be universal among lepidopterous insects.
authors.affiliation: National Research Council of Canada, Biotechnology Research Institute, Montreal, Quebec, Canada.
authors.forename: M
authors.initials: M
authors.lastname: van Munster
authors.forename: G
authors.initials: G
authors.lastname: Préfontaine
authors.forename: L
authors.initials: L
authors.lastname: Meunier
authors.forename: M
authors.initials: M
authors.lastname: Elias
authors.forename: A
authors.initials: A
authors.lastname: Mazza
authors.forename: R
authors.initials: R
authors.lastname: Brousseau
authors.forename: L
authors.initials: L
authors.lastname: Masson
mesh_terms: D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000494:pharmacology*; D001427:Bacterial Toxins / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004731:Endotoxins / Q000494:pharmacology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D006460:Hemolysin Proteins / Q000494:pharmacology*; D007306:Insecticides / Q000494:pharmacology; D007814:Larva / Q000187:drug effects / Q000235:genetics; D009036:Moths / Q000187:drug effects* / Q000235:genetics*
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis