title: Characterization of a petunia acetyltransferase involved in the biosynthesis of the floral volatile isoeugenol.
abstract: Petunia flower petals emit large amounts of isoeugenol, which has been shown to be synthesized by isoeugenol synthase (PhIGS1) from an ester of coniferyl alcohol, hypothesized to be coniferyl acetate. This paper describes the identification and characterization of a novel petunia gene encoding an enzyme belonging to the BAHD acyltransferase family whose expression correlates with isoeugenol biosynthesis. RNAi suppression of this gene results in inhibition of isoeugenol biosynthesis. Biochemical characterization of the protein encoded by this gene showed that it has acetyltransferase activity and is most efficient with coniferyl alcohol among the alcohol substrates tested. Overall, these data support the conclusion that coniferyl acetate is the substrate of isoeugenol synthase.
authors.affiliation: Department of Environmental Horticulture, University of Florida, Gainesville, FL 32611, USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Dexter
authors.forename: Anthony
authors.initials: A
authors.lastname: Qualley
authors.forename: Christine M
authors.initials: CM
authors.lastname: Kish
authors.forename: Choong Je
authors.initials: CJ
authors.lastname: Ma
authors.forename: Takao
authors.initials: T
authors.lastname: Koeduka
authors.forename: Dinesh A
authors.initials: DA
authors.lastname: Nagegowda
authors.forename: Natalia
authors.initials: N
authors.lastname: Dudareva
authors.forename: Eran
authors.initials: E
authors.lastname: Pichersky
authors.forename: David
authors.initials: D
authors.lastname: Clark
mesh_terms: D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005054:Eugenol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D035264:Flowers / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D015394:Molecular Structure; D032306:Petunia / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D034622:RNA Interference; D011994:Recombinant Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013379:Substrate Specificity
chemical_list: D018076:DNA, Complementary; D011994:Recombinant Proteins; D005054:Eugenol; C036643:isoeugenol; D000123:Acetyltransferases