[Role of dendritic cells in graft rejection after penetrating keratoplasty].
OBJECTIVE
To gain insight into the role of dendritic cells in graft rejection following penetrating keratoplasty by investigating their distribution in rat cornea.


METHODS
Orthotopical corneal transplantation was performed and immunohistochemical staining of the whole-mount cornea and the spleen tissue specimen employed to determine the distribution of the dendritic cells in the cornea.


RESULTS
Graft rejection occurred in all rats following the transplantation. No OX-62(+) dendritic cells were found in normal cornea but they were present in the epithelium of the cornea graft with allograft rejection.


CONCLUSIONS
OX-62(+) dendritic cells presenting in the rejected cornea may be related to acute graft rejection after penetrating keratoplasty.
Bai
Lang
L
Department of Ophthalmology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. bailangsfy@163.com
Lu
Xiao-He
XH
Dang
Sen-Tao
ST
Zhou
Jin
J
D000818:Animals; D003315:Cornea / Q000276:immunology / Q000473:pathology / Q000601:surgery; D003713:Dendritic Cells / Q000276:immunology* / Q000502:physiology; D005260:Female; D006084:Graft Rejection / Q000209:etiology / Q000276:immunology* / Q000503:physiopathology; D015948:Keratoplasty, Penetrating / Q000009:adverse effects / Q000379:methods*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar