Knockout mutagenesis of the kpsE gene of Campylobacter jejuni 81116 and its involvement in bacterium-host interactions.
Campylobacter jejuni is a common cause of bacterial enteritis. The surface capsular polysaccharides are important for this bacterium to survive in the environment, but little is known about their involvement in bacterium-host interactions. This study showed that the C. jejuni capsular polysaccharides play an important role in adherence to and invasion of human embryonic epithelial cells. However, no significant role of capsular polysaccharides was shown in colonization of the chicken gut.
Bachtiar
Boy M
BM
Biotechnology and Environmental Biology, RMIT University, Melbourne, Australia.
Coloe
Peter J
PJ
Fry
Benjamin N
BN
D000818:Animals; D001422:Bacterial Adhesion; D016667:Bacterial Capsules / Q000096:biosynthesis / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002169:Campylobacter Infections / Q000382:microbiology; D016123:Campylobacter jejuni / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D002645:Chickens; D020868:Gene Silencing; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D016297:Mutagenesis, Site-Directed; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000235:genetics
D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; D011135:Polysaccharides, Bacterial