title: Microscopic detection of thermogenesis in a single HeLa cell.
abstract: We report here the technique for detection and measurement of the temperature changes in single cells using a recently devised microthermometer (a glass micropipette filled with the thermosensitive fluorescent dye Europium (III) thenoyltrifluoroacetonate trihydrate). We found that the heat production in a single HeLa cell occurred with some time delay after the ionomycin-induced Ca(2+) influx from the extracellular space. The time delay inversely depended on extracellular [Ca(2+)], and the increase in temperature was suppressed when Ca(2+)-ATPases were blocked by thapsigargin. These observations strongly suggest that the enzymatic activity of Ca(2+)-ATPases in endoplasmic reticulum leads to the heat production. This study has therefore paved the way for studying the thermogenesis at the single-cell level.
authors.forename: Madoka
authors.initials: M
authors.lastname: Suzuki
authors.forename: Vadim
authors.initials: V
authors.lastname: Tseeb
authors.forename: Kotaro
authors.initials: K
authors.lastname: Oyama
authors.forename: Shin'ichi
authors.initials: S
authors.lastname: Ishiwata
mesh_terms: D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*; D022722:Thermogenesis / Q000502:physiology*; D013817:Thermography / Q000295:instrumentation / Q000379:methods*