Stability of synthetic exendin-4 in human plasma in vitro.
Sites of exendin-4 that that are relatively susceptible to degradation in plasma were identified with the aim of providing information for designing new exendin-4 analogues. The stability of exendin-4 in human plasma was evaluated in vitro. The results showed that the peptide was slowly degraded with a half-life of 9.57 h and the principal cleavage sites are between Thr5 and Phe6, Phe6 and Thr7, and Thr7 and Ser8 of the N-terminus region of exendin-4.
Chen
Jie
J
College of Life Science, Jilin University, Changchun 130012, P.R. China.
Yu
Ling
L
Wang
Liping
L
Fang
Xuexun
X
Li
Li
L
Li
Wei
W
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D000077270:Exenatide; D052216:Glucagon-Like Peptide 1 / Q000737:chemistry; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000097:blood* / Q000737:chemistry*; D017433:Protein Structure, Secondary; D014688:Venoms / Q000097:blood* / Q000737:chemistry*
D010455:Peptides; D014688:Venoms; D052216:Glucagon-Like Peptide 1; D000077270:Exenatide