1H MRS signals from glutathione may act as predictive markers of apoptosis in irradiated tumour cells.
Inhibition of apoptosis in tumour cells may depend on intracellular reduced glutathione (GSH) level. In this work, GSH levels were studied by (1)H MRS in MCF-7 and HeLa cells, characterised by a different radiosensitivity. Annexin-V test showed that the fraction of apoptotic HeLa cells after irradiation is much higher than in control, although MCF-7 cells did not show a significant apoptosis. MRS signals from GSH (G) show lower intensity in HeLa with respect to MCF-7 cells; the opposite is true for free glutamic acid [glu (g)]. After irradiation, the G/g ratio decreases in MCF-7, although remaining approximately constant in HeLa cells. Buthionine sulfoximine (BSO) treated MCF-7 cells show an increase in the percentage of apoptotic cells; in parallel, G/g ratio behaves as in HeLa. This study indicates that GSH level may act as predictive marker of apoptosis by irradiation.
Grande
S
S
Department of Technology and Health and INFN Sanità Group, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Roma, Italy.
Giovannini
C
C
Guidoni
L
L
Luciani
A M
AM
Palma
A
A
Rosi
A
A
Sapora
O
O
Viti
V
V
D017209:Apoptosis / Q000528:radiation effects*; D014408:Biomarkers, Tumor / Q000032:analysis*; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D004307:Dose-Response Relationship, Radiation; D005978:Glutathione / Q000032:analysis*; D006367:HeLa Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011522:Protons; D011829:Radiation Dosage; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D014408:Biomarkers, Tumor; D011522:Protons; D005978:Glutathione