Effect of heterologous expression of phaG [(R)-3-hydroxyacyl-ACP-CoA transferase] on polyhydroxyalkanoate accumulation from the aromatic hydrocarbon phenylacetic acid in Pseudomonas species.
Five Pseudomonas strains capable of growth with the aromatic carboxylic acid phenylacetic acid were investigated with a view to improving PHA accumulation. The overexpression of (R)-3-hydroxyacyl-ACP-CoA transferase (PhaG) from Pseudomonas putida CA-3 increased PHA accumulation in only one of the five strains tested, namely Pseudomonas jessenii C8. Recombinant P. jessenii C8 harbouring the phaG gene showed a 4.1-fold increase (9.6-39% cell dry weight) in PHA accumulation when grown on phenylacetic acid (15 mM) compared with the wild-type strain. This is the highest reported level of PHA accumulation from phenylacetic acid. This is also the first time the heterologous expression of phaG has resulted in improved PHA accumulation from an aromatic carbon source. The growth patterns of the wild type and recombinant strains were very similar, with no significant differences observed in carbon and nitrogen utilization.
Tobin
Karen M
KM
School of Biomolecular and Biomedical Sciences, College of Life Sciences, and Centre for Synthesis and Chemical Biology, The Conway Institute for Biomolecular and Biomedical research, University College Dublin, Belfield, Dublin, Ireland.
O'Leary
Niall D
ND
Dobson
Alan D W
AD
O'Connor
Kevin E
KE
D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D015964:Gene Expression Regulation, Bacterial; D006841:Hydrocarbons, Aromatic / Q000378:metabolism*; D010648:Phenylacetates / Q000378:metabolism*; D011091:Polyesters / Q000378:metabolism*; D011549:Pseudomonas / Q000145:classification / Q000235:genetics / Q000254:growth & development; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D006841:Hydrocarbons, Aromatic; D010648:Phenylacetates; D011091:Polyesters; D011994:Recombinant Proteins; D000217:Acyltransferases; C114682:3-hydroxyacyl-CoA-acyl carrier protein transferase; C025136:phenylacetic acid