title: The impact of polymerase chain reaction assays for the detection of hepatitis C virus infection in a hemodialysis unit.
abstract: Hepatitis C virus (HCV) infection is most often diagnosed by detection of antibodies against the virus (HCV Ab). However, it has been reported that some HCV Ab negative patients test positive for HCV-RNA. Over a study period of 30 months, all patients on hemodialysis at the Al Hada Armed Forces Hospital in Taif, Saudi Arabia were tested monthly for HCV Ab and twice per year for HCV-RNA. HCV Ab was tested by a third generation microparticle enzyme immunoassay (MEIA), and HCV-RNA by a qualitative hepatitis-RNA assay, second version (COBAS Amplicor PCR), which was recently introduced in the Molecular Pathology Laboratory of our hospital. Of the 180 patients studied, 34 (18.9%) had positive HCV Ab, and of the 146 HCV Ab negative patients, five patients tested positive for HCV-RNA (3.42%). Our study further finds that, when applying HCV Ab testing only, some patients with HCV viremia may be undetected. For better HCV infection control, routine HCV-RNA testing of dialysis patients should be considered, particularly in areas where the infection is common and in units applying isolation policies.
authors.affiliation: Department of Nephrology and Dialysis, Al Hada Armed Forces Hospital, Taif, Kingdom of Saudi Arabia.
authors.forename: Magdi M
authors.initials: MM
authors.lastname: Hussein
authors.forename: Jaap M
authors.initials: JM
authors.lastname: Mooij
authors.forename: Mohamed S
authors.initials: MS
authors.lastname: Hegazy
authors.forename: Mohammed S
authors.initials: MS
authors.lastname: Bamaga
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D005260:Female; D006436:Hemodialysis Units, Hospital; D016174:Hepacivirus / Q000235:genetics*; D006526:Hepatitis C / Q000175:diagnosis* / Q000635:transmission / Q000821:virology; D018937:Hepatitis C Antibodies / Q000097:blood; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods; D017053:Infection Control / Q000379:methods; D008297:Male; D008403:Mass Screening / Q000379:methods*; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests; D012367:RNA, Viral / Q000097:blood*; D006435:Renal Dialysis / Q000009:adverse effects*; D012529:Saudi Arabia; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D018937:Hepatitis C Antibodies; D012367:RNA, Viral