Analysis on the factors affecting start-up intensity in the upstream sequence of phycocyanin beta subunit gene from Arthrospira platensis by site-directed mutagenesis.
Six promoters in the 419 bp upstream sequence of the phycocyanin beta subunit gene of Arthrospira platensis FACHB341 have been previously cloned. Site-directed mutagenesis has now been used to introduce mutations in the -10 and -35 boxes of promoter 3, -10 box of promoter 4, and -35 box of promoter 6. The expression level of green fluorescent protein gene was measured by flow cytometry. Results showed that the effects of site-directed mutagenesis in different promoters were dissimilar: some increased and some declined.
Guo
Nan
N
College of Marine Life Sciences, Ocean University of China, Qingdao, PR China.
Zhang
Xuecheng
X
Lu
Yongzhong
Y
Song
Xiaojin
X
D001483:Base Sequence; D000458:Cyanobacteria / Q000235:genetics*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D010798:Phycocyanin / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D015202:Protein Engineering / Q000379:methods*; D021122:Protein Subunits / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*
D021122:Protein Subunits; D010798:Phycocyanin