title: Glucose biosensor based on nano-SiO2 and "unprotected" Pt nanoclusters.
abstract: The "unprotected" Pt nanoclusters (average size 2 nm) mixed with the nanoscale SiO(2) particles (average size 13 nm) were used as a glucose oxidase immobilization carrier to fabricate the amperometric glucose biosensor. The bioactivity of glucose oxidase (GOx) immobilized on the composite was maintained and the as-prepared biosensor demonstrated high sensitivity (3.85 microA mM(-1)) and good stability in glucose solution. The Pt-SiO(2) biosensor showed a detection limit of 1.5 microM with a linear range from 0.27 to 4.08 mM. In addition, the biosensor can be operated under wide pH range (pH 4.9-7.5) without great changes in its sensitivity. Cyclic voltammetry measurements showed a mixed controlled electrode reaction.
authors.affiliation: Department of Chemistry, Tsinghua University, Beijing 100084, PR China.
authors.forename: Haipeng
authors.initials: H
authors.lastname: Yang
authors.forename: Yongfa
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry; D004566:Electrodes; D005947:Glucose / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D053758:Nanoparticles / Q000737:chemistry*; D010984:Platinum; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D010984:Platinum; D012822:Silicon Dioxide; D005947:Glucose