A role for proteoglycans in mineralized tissue-titanium adhesion.
Biomechanical properties of the bone-titanium interface have rarely been studied, due to the technical limitations involved; whether biological bonding mechanisms exist has not been determined. We hypothesized that a selected set of proteoglycan/glycosaminoglycan complexes plays a role in establishing the adhesion between bone and titanium, and utilized the rat bone-marrow-derived osteoblastic culture model to gain an insight into the hypothesis. Gene expression of selected proteoglycan core proteins was up-regulated in the osteoblasts cultured on titanium compared with those on polystyrene. Various sulfated glycosaminoglycans were immunochemically localized at mineralized tissue-titanium interfaces. The administration of various glycosaminoglycan-degrading enzymes into the cultures resulted in a 25-45% reduction of the tissue-titanium interfacial strength, measured by a nanoscratch test; while the hardness and elastic modulus of the mineralized tissue, evaluated by nano-indentation, were not altered. In conclusion, glycosaminoglycan degradation resulted in a decreased interfacial strength between cultured mineralized tissue and titanium, but did not alter the intrinsic strength of the mineralized tissue, suggesting a role for proteoglycan/glycosaminoglycan complexes in the establishment of tissue-titanium adhesion.
Nakamura
H K
HK
The Jane and Jerry Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, Biomaterials and Hospital Dentistry, UCLA School of Dentistry, Los Angeles, California 90095-1668, USA.
Butz
F
F
Saruwatari
L
L
Ogawa
T
T
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002478:Cells, Cultured; D003799:Dental Stress Analysis; D004548:Elasticity; D015870:Gene Expression; D006025:Glycosaminoglycans / Q000032:analysis / Q000378:metabolism*; D006244:Hardness; D008297:Male; D016348:Osseointegration / Q000502:physiology*; D010006:Osteoblasts / Q000378:metabolism*; D011509:Proteoglycans / Q000096:biosynthesis / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014025:Titanium
D006025:Glycosaminoglycans; D011509:Proteoglycans; D014025:Titanium