title: Dilution of dye improves parametrial SLN detection in patients with cervical cancer.
abstract: OBJECTIVE
  Aim of this study to improve the detection rate of parametrial sentinel nodes in patients with early cervical cancer by using a new dosage of blue dye in a randomized trial.
  
  
  METHODS
  Standard labelling volume of 4 ml Patent Blue was compared to 2 ml Patent Blue diluted with 8 ml NaCl 0.9% in 60 patients using a randomized protocol. Tc-99 was not applied in any patient. All patients underwent open lymphadenectomy.
  
  
  RESULTS
  In each arm 30 patients were enrolled. Overall detection rate of sentinel nodes was 93.3%. Both groups did not differ with regard to patient's age, BMI, tumor stage, number of lymph nodes harvested, number of sentinel nodes detected and detection rate. Significantly more patients with parametrial sentinel nodes were detected in the diluted protocol (37.0% vs. 10.3%) which is due to the lateral part of the cardinal ligament (29.6% vs. 6.9%).
  
  
  CONCLUSIONS
  In our study overall detection of sentinel nodes using a dye was high. The diluted solution of Patent Blue led to a higher detection rate of parametrial sentinel nodes. These findings might help explain the discrepancy between the high reported rate of parametrial lymph nodes in anatomical studies compared with the low rates in clinical sentinel series.
authors.affiliation: Department of Obstetrics and Gynecology, University Hospital of Schleswig-Holstein, Campus Lübeck, Ratzeburger Allee 160, 23538 Luebeck, Germany. altgassen@frauenklinik.uni-luebeck.de
authors.forename: C
authors.initials: C
authors.lastname: Altgassen
authors.forename: A
authors.initials: A
authors.lastname: Paseka
authors.forename: H
authors.initials: H
authors.lastname: Urbanczyk
authors.forename: T
authors.initials: T
authors.lastname: Dimpfl
authors.forename: K
authors.initials: K
authors.lastname: Diedrich
authors.forename: G
authors.initials: G
authors.lastname: Dahmen
authors.forename: H
authors.initials: H
authors.lastname: Hertel
mesh_terms: D000328:Adult; D000368:Aged; D004396:Coloring Agents; D005260:Female; D006801:Humans; D008197:Lymph Node Excision; D008198:Lymph Nodes / Q000473:pathology* / Q000601:surgery; D008207:Lymphatic Metastasis; D008875:Middle Aged; D012394:Rosaniline Dyes; D021701:Sentinel Lymph Node Biopsy / Q000379:methods*; D002583:Uterine Cervical Neoplasms / Q000473:pathology* / Q000601:surgery
chemical_list: D004396:Coloring Agents; D012394:Rosaniline Dyes; C008769:sulfan blue