title: Phosphoinositides specify polarity during epithelial organ development.
abstract: The molecular mechanisms that integrate cellular polarity with tissue architecture during epithelial morphogenesis are poorly understood. Using a three-dimensional model of epithelial morphogenesis, report that the phosphatase PTEN and phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] regulate the GTPase Cdc42 and the kinase aPKC to generate the apical plasma membrane domain and maintain apical-basolateral polarity.
authors.affiliation: Laboratory of Cellular and Molecular Biology, Center for Cancer Research, NCI, NIH, 37 Convent Drive, Bldg. 37, Room 2066, Bethesda, MD 20892, USA.
authors.forename: Frank I
authors.initials: FI
authors.lastname: Comer
authors.forename: Carole A
authors.initials: CA
authors.lastname: Parent
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D016764:Cell Polarity / Q000502:physiology*; D004848:Epithelium / Q000196:embryology / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D038081:Organogenesis / Q000502:physiology*; D051059:PTEN Phosphohydrolase / Q000378:metabolism; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D020764:cdc42 GTP-Binding Protein / Q000378:metabolism
chemical_list: D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols; C115015:PKC-3 protein; D011493:Protein Kinase C; D051059:PTEN Phosphohydrolase; D020764:cdc42 GTP-Binding Protein