title: Effectiveness of novel PGM-containing incomplete Seppic adjuvants in rabbits.
abstract: Peptidoglycan monomer (PGM) is adjuvant active molecule in experimental mice, although its adjuvanticity is much lower in comparison to potent adjuvants. The novel adjuvant formulations were developed by incorporation of PGM into Montanide ISA 206 and Montanide ISA 720 adjuvants, with the aim to enhance its adjuvanticity by protecting it from the fast degradation and metabolic clearance. Adjuvanticity of the novel adjuvant formulations was tested in rabbits for induction of protein-specific antibodies. Both novel adjuvants ISA206(PGM) and ISA720(PGM) were significantly stronger than Montanide adjuvants themselves, and also significantly more potent than Complete Freund Adjuvant. Montanide ISA 720 was shown as much better carrier of PGM, since the novel ISA720(PGM) adjuvant was significantly stronger adjuvant than the ISA206(PGM).
authors.affiliation: Research and Development Department, Institute of Immunology, Inc., Rockefellerova 10, HR-10000 Zagreb, Croatia. bhalassy@imz.hr
authors.forename: Beata
authors.initials: B
authors.lastname: Halassy
authors.forename: Valerija
authors.initials: V
authors.lastname: Vdović
authors.forename: Lidija
authors.initials: L
authors.lastname: Habjanec
authors.forename: Maja Lang
authors.initials: ML
authors.lastname: Balija
authors.forename: Branka
authors.initials: B
authors.lastname: Gebauer
authors.forename: Ante
authors.initials: A
authors.lastname: Sabioncello
authors.forename: Tomislav
authors.initials: T
authors.lastname: Santek
authors.forename: Jelka
authors.initials: J
authors.lastname: Tomasić
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D008353:Mannitol / Q000031:analogs & derivatives* / Q000494:pharmacology; D009829:Oleic Acids / Q000494:pharmacology*; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology; D010457:Peptidoglycan / Q000494:pharmacology*; D011817:Rabbits
chemical_list: D000276:Adjuvants, Immunologic; C408982:CPG-oligonucleotide; D007074:Immunoglobulin G; D009829:Oleic Acids; D009838:Oligodeoxyribonucleotides; D010457:Peptidoglycan; C001681:mannide monooleate; D008353:Mannitol; C032972:peptidoglycan pentapeptide-mDap3