Intrahepatic interleukin-8 production during disease progression of chronic hepatitis C.
The current study was designed to investigate the contribution of chemokines to the pathogenesis of chronic hepatitis C and hepatocellular carcinoma (HCC) by measuring the production of IL-8, monocyte chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-1 alpha (MIP-1alpha). A solid-phase sandwich enzyme-linked immunosorbent assay (ELISA) was established to quantitate serum concentrations of the chemokines. Expression of chemokines in liver tissues was evaluated immunohistochemically using specific monoclonal antibodies. As the severity of chronic hepatitis escalated, serum IL-8 levels increased progressively. Moreover, in the hepatocellular carcinoma (HCC) patients, IL-8 concentrations were positively correlated with the macroscopic staging of HCC, and inversely correlated with the duration of the survival periods. The results demonstrate that IL-8 production may be augmented upon the malignant transformation of hepatocytes in chronic hepatitis C.
Tachibana
Yoshiya
Y
Department of Gastroenterology, Graduate School of Medical Science, Kanazawa University, Kanazawa 920-8641, Ishikawa, Japan.
Nakamoto
Yasunari
Y
Mukaida
Naofumi
N
Kaneko
Shuichi
S
D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000097:blood / Q000473:pathology; D018932:Chemokine CCL2 / Q000097:blood; D054405:Chemokine CCL3; D019742:Chemokines, CC / Q000097:blood; D018450:Disease Progression; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D019698:Hepatitis C, Chronic / Q000097:blood / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D016209:Interleukin-8 / Q000096:biosynthesis / Q000097:blood*; D008099:Liver / Q000378:metabolism / Q000473:pathology* / Q000821:virology; D008113:Liver Neoplasms / Q000097:blood / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D016019:Survival Analysis
C111835:CCL2 protein, human; C515429:CCL3 protein, human; D018932:Chemokine CCL2; D054405:Chemokine CCL3; D019742:Chemokines, CC; D016209:Interleukin-8