title: Novel membrane-localizing TEMPO derivatives for measurement of cellular oxidative stress at the cell membrane.
abstract: Oxidative stress affecting lipid membranes is considered to be closely related to cardiovascular disease and brain ischemia. In this study, we designed and synthesized membrane-localizing TEMPO derivatives and demonstrated that one of these synthesized probes, compound 1, localized and detected oxidative stress in the cell membrane in an endotoxic model of a mouse macrophage-like cell line. Compound 1 is therefore a potentially useful probe for evaluating oxidative stress at the cell membrane.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya, Aichi 467-8603, Japan.
authors.forename: Shizuka
authors.initials: S
authors.lastname: Ban
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Nakagawa
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Naoki
authors.initials: N
authors.lastname: Miyata
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000473:pathology; D002318:Cardiovascular Diseases / Q000473:pathology; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D003497:Cyclic N-Oxides / Q000138:chemical synthesis*; D015195:Drug Design; D004731:Endotoxins; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D018384:Oxidative Stress; D013113:Spin Labels / Q000138:chemical synthesis*; D013329:Structure-Activity Relationship
chemical_list: D003497:Cyclic N-Oxides; D004731:Endotoxins; D013113:Spin Labels; C003959:TEMPO