Micro-scale analysis of aminoglycoside antibiotics in human plasma by capillary liquid chromatography and nanospray tandem mass spectrometry with column switching.
A simple liquid chromatographic method combined with tandem mass spectrometry (LC-MS-MS) is described for the analysis of aminoglycoside antibiotics. Clinically these antibiotics may cause both ototoxicity and nephrotoxicity; therefore, the monitoring of aminoglycoside levels in patient plasma is required for protecting human health. In this study separation of the method is based on ion-pair chromatographic technology on a short capillary reversed-phase C18 column. The method was successfully applied to analyze amikacin in human plasma. In human plasma after deproteinisation with HFBA, an aliquot of 1 microL supernatant was injected into the chromatographic system. Only a small amount of plasma sample, 10 microL, is sufficient for the monitoring of amikacin levels in clinically therapeutic range. The relative standard deviations (RSD) of the method for intra- and inter-day analyses (n=5) are less than 5.8%. Application of this method for the trace analysis of amikacin in human plasma proved simple and workable.
Lu
Chi-Yu
CY
Proteomics Research Center, National Yang-Ming University, Taipei 115, Taiwan.
Feng
Chia-Hsien
CH
D000617:Aminoglycosides / Q000097:blood*; D000900:Anti-Bacterial Agents / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D036103:Nanotechnology / Q000379:methods; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents