title: [Theory, principles and applications of fluorescent technologies in cellular biology and cancer research].
abstract: Using fluorescent technologies under the microscope is becoming unavoidable in biology research laboratories. These technologies, stemming from instrumental developments of biophysics, raised with the use of fluorescent proteins derived from the GFP (green fluorescent protein) as well as the market development of commercial instruments. They allow studying the dynamics of molecules of interests (FRAP, FCS...) as well as their interactions (FRET, FLIM...) on cells, cellular extracts or tissues. Unfortunately, these are often used without detailed knowledge of their principles as well as their theories. These aspects are developed and illustrated here through means of literature example.
authors.affiliation: CNRS, Université de Nice, PRISM, Institute of Signaling Developmental Biology and Cancer, UMR6543 Centre de Biochimie, Faculté des Sciences, 06108 Nice Cedex 2.
authors.forename: Cedric
authors.initials: C
authors.lastname: Matthews
authors.forename: Cyril
authors.initials: C
authors.lastname: Favard
mesh_terms: D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000235:genetics; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D012106:Research
chemical_list: D049452:Green Fluorescent Proteins