title: A microfluidic device for practical label-free CD4(+) T cell counting of HIV-infected subjects.
abstract: Practical HIV diagnostics are urgently needed in resource-limited settings. While HIV infection can be diagnosed using simple, rapid, lateral flow immunoassays, HIV disease staging and treatment monitoring require accurate counting of a particular white blood cell subset, the CD4(+) T lymphocyte. To address the limitations of current expensive, technically demanding and/or time-consuming approaches, we have developed a simple CD4 counting microfluidic device. This device uses cell affinity chromatography operated under differential shear flow to specifically isolate CD4(+) T lymphocytes with high efficiency directly from 10 microliters of unprocessed, unlabeled whole blood. CD4 counts are obtained under an optical microscope in a rapid, simple and label-free fashion. CD4 counts determined in our device matched measurements by conventional flow cytometry among HIV-positive subjects over a wide range of absolute CD4 counts (R(2) = 0.93). This CD4 counting microdevice can be used for simple, rapid and affordable CD4 counting in point-of-care and resource-limited settings.
authors.affiliation: Surgical Services and Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, and Shriners Hospital for Children, Boston, Massachusetts 02114, USA.
authors.forename: Xuanhong
authors.initials: X
authors.lastname: Cheng
authors.forename: Daniel
authors.initials: D
authors.lastname: Irimia
authors.forename: Meredith
authors.initials: M
authors.lastname: Dixon
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Sekine
authors.forename: Utkan
authors.initials: U
authors.lastname: Demirci
authors.forename: Lee
authors.initials: L
authors.lastname: Zamir
authors.forename: Ronald G
authors.initials: RG
authors.lastname: Tompkins
authors.forename: William
authors.initials: W
authors.lastname: Rodriguez
authors.forename: Mehmet
authors.initials: M
authors.lastname: Toner
mesh_terms: D015704:CD4 Antigens / Q000096:biosynthesis; D018791:CD4 Lymphocyte Count / Q000379:methods*; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism* / Q000821:virology; D002448:Cell Adhesion; D002846:Chromatography, Affinity; D005434:Flow Cytometry / Q000379:methods; D015658:HIV Infections / Q000097:blood*; D006679:HIV Seropositivity / Q000097:blood*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D013499:Surface Properties; D013997:Time Factors
chemical_list: D015704:CD4 Antigens