title: Inhibition by epigallocatechin gallate of CoCl2-induced apoptosis in rat PC12 cells.
abstract: Epigallocatechin-3-gallate (EGCG) is a major constituent of green tea polyphenols. This study was aimed to investigate the possible mechanisms of EGCG-mediated inhibition against apoptosis in rat pheochromocytoma PC12 cells by exposure to CoCl(2). Exposure to CoCl(2) caused the generation of ROS and induced cell death with appearance of apoptotic morphology and DNA fragmentation. However, EGCG rescued the loss of viability in the cells exposed to CoCl(2) and led the reduction of DNA fragmentation and sub-G(1) fraction of cell cycle. Also, EGCG attenuated the CoCl(2)-induced disruption of mitochondrial membrane potential (DeltaPsim), release of cytochrome c from the mitochondria to cytosol and abolished the CoCl(2)-stimulated activities of the caspase cascades, caspase-9 and caspase-3. In addition, EGCG ameliorated the increase in the Bax to Bcl-2 ratio, a marker of apoptosis proceeding, induced by CoCl(2) treatment. Taken together, the present results suggest that EGCG inhibit the CoCl(2)-induced apoptosis of PC12 cells through the mitochondria-mediated apoptosis pathway involved in modulating the Bcl-2 family.
authors.affiliation: Dental Science Research Institute, School of Dentistry, 2nd Stage of Brain Korea 21 for School of Dentistry, Chonnam National University, Gwang Ju 500-757, South Korea.
authors.forename: Ji-Yeon
authors.initials: JY
authors.lastname: Jung
authors.forename: Hyun-Chul
authors.initials: HC
authors.lastname: Mo
authors.forename: Kyu-Ho
authors.initials: KH
authors.lastname: Yang
authors.forename: Yeon-Jin
authors.initials: YJ
authors.lastname: Jeong
authors.forename: Hyeon-Gyeong
authors.initials: HG
authors.lastname: Yoo
authors.forename: Nam-Ki
authors.initials: NK
authors.lastname: Choi
authors.forename: Won-Mann
authors.initials: WM
authors.lastname: Oh
authors.forename: Hee-Kyun
authors.initials: HK
authors.lastname: Oh
authors.forename: Sun-Hun
authors.initials: SH
authors.lastname: Kim
authors.forename: Jin-Ha
authors.initials: JH
authors.lastname: Lee
authors.forename: Hyun-Jin
authors.initials: HJ
authors.lastname: Kim
authors.forename: Won-Jae
authors.initials: WJ
authors.lastname: Kim
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D003035:Cobalt / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D053938:DNA Fragmentation / Q000187:drug effects; D004587:Electrophoresis, Agar Gel; D005434:Flow Cytometry; D019254:Genes, bcl-2 / Q000235:genetics; D020925:Hypoxia-Ischemia, Brain / Q000473:pathology; D008564:Membrane Potentials / Q000187:drug effects; D051336:Mitochondrial Membranes / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016716:PC12 Cells; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; D013778:Tetrazolium Salts; D013844:Thiazoles; D003035:Cobalt; D002392:Catechin; C045651:epigallocatechin gallate; D053148:Caspase 3; D053453:Caspase 9; C022616:thiazolyl blue; C018021:cobaltous chloride