Improved calcium imaging in transgenic mice expressing a troponin C-based biosensor.
Fluorescent Ca(2+) indicator proteins (FCIPs) are attractive tools for studying Ca(2+) dynamics in live cells. Here we describe transgenic mouse lines expressing a troponin C (TnC)-based biosensor. The biosensor is widely expressed in neurons and has improved Ca(2+) sensitivity both in vitro and in vivo. This allows FCIP-based two-photon Ca(2+) imaging of distinct neurons and their dendrites in vivo, and opens a new avenue for structure-function analysis of intact neuronal circuits.
Heim
Nicola
N
Max Planck Institute of Neurobiology, Am Klopferspitz 18, 82152 Martinsried, Germany.
Garaschuk
Olga
O
Friedrich
Michael W
MW
Mank
Marco
M
Milos
Ruxandra I
RI
Kovalchuk
Yury
Y
Konnerth
Arthur
A
Griesbeck
Oliver
O
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D001921:Brain / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D003712:Dendrites / Q000378:metabolism; D005260:Female; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D009474:Neurons / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D019209:Troponin C / Q000235:genetics / Q000378:metabolism*
C485182:Cyan Fluorescent Protein; D019209:Troponin C; D049452:Green Fluorescent Proteins; D002118:Calcium