Maid (GCIP) is involved in cell cycle control of hepatocytes.
The function of Maid (GCIP), a cyclinD-binding helix-loop-helix protein, was analyzed by targeted disruption in mice. We show that Maid function is not required for normal embryonic development. However, older Maid-deficient mice-in contrast to wild-type controls--develop hepatocellular carcinomas. Therefore, we studied the role of Maid during cell cycle progression after partial hepatectomy (PH). Lack of Maid expression after PH was associated with a delay in G1/S-phase progression as evidenced by delayed cyclinA expression and DNA replication in Maid-deficient mice. However, at later time points liver mass was restored normally.


CONCLUSIONS
These results indicate that Maid is involved in G1/S-phase progression of hepatocytes, which in older animals is associated with the development of liver tumors.
Sonnenberg-Riethmacher
Eva
E
ZMNH (Centre for Molecular Neurobiology) University of Hamburg, Hamburg, Germany.
Wüstefeld
Torsten
T
Miehe
Michaela
M
Trautwein
Christian
C
Riethmacher
Dieter
D
D018248:Adenoma, Liver Cell / Q000473:pathology; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology*; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D002455:Cell Division / Q000235:genetics / Q000502:physiology; D047108:Embryonic Development / Q000235:genetics / Q000502:physiology; D005260:Female; D022781:Hepatocytes / Q000166:cytology / Q000502:physiology*; D008099:Liver / Q000166:cytology / Q000196:embryology / Q000502:physiology*; D008113:Liver Neoplasms / Q000473:pathology; D008115:Liver Regeneration / Q000502:physiology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009154:Mutation / Q000235:genetics; D011247:Pregnancy; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*
C107359:Ccndbp1 protein, mouse; D014157:Transcription Factors