Immunohistochemical and molecular study of severe cervical dysplasia associated with HPV-83.
BACKGROUND
Based on epidemiological data, infections with specific types of HPV can be classified as high-risk (HR), low-risk (LR) or intermediate-risk (IR) HPV depending on the risk of progression to cervical cancer.


METHODS
A 70-year-old woman consulted for relapse of abnormal cytology with high-grade squamous intraepithelial lesions (HSIL) associated with HPV-83 infection. Histological examination demonstrated high-grade cervical intraepithelial neoplasia (CIN III) with strong and diffuse staining by the P16(INK4a)-specific antibody.


CONCLUSIONS
This patient's intermediate-risk HPV infection (HPV-83) rapidly progressed to severe cervical intraepithelial neoplasia (CIN III) with strong anti-P16(INK4a) immunolabelling. Analysis of the E6 peptide sequence revealed several mutations in one of the two putative zinc finger regions (AA residues 107-135) associated with p53 binding.
Albrecht
Valérie
V
Laboratoire de Virologie, BP 3079, Hôpital de l'Archet, 151 route de Saint-Antoine de Ginestière, 06200 Nice cedex 3, France.
Chevallier
Anne
A
Bongain
André
A
Lefebvre
Jean-Claude
JC
Giordanengo
Valérie
V
D000368:Aged; D001483:Base Sequence; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000821:virology*; D002578:Uterine Cervical Dysplasia / Q000821:virology*; D002583:Uterine Cervical Neoplasms / Q000821:virology*