title: Vesicular monoamine transporter 1 mediates dopamine secretion in rat proximal tubular cells.
abstract: Renal dopamine, synthesized by proximal tubules, plays an important role in the regulation of renal sodium excretion. Although the renal dopaminergic system has been extensively investigated in both physiological and pathological situations, the mechanisms whereby dopamine is stored and secreted by proximal tubule cells remain obscure. In the present study we investigated whether vesicular monoamine transporters (VMAT)-1 and -2, which participate in amine storing and secretion, are expressed in rat renal proximal tubules, and we defined their involvement in dopamine secretion. By combining RT-PCR, Western blot, and immunocytochemistry we showed that VMAT-1 is the predominant isoform expressed in isolated proximal tubule cells. These results were confirmed by immunohistochemistry analysis of rat renal cortex showing that VMAT-1 was found in proximal tubules but not in glomeruli. Functional studies showed that, as previously reported for VMAT-dependent amine transporters, dopamine release by cultured proximal tubule cells was partially inhibited by disruption of intracellular H(+) gradient. In addition, dopamine secretion was prevented by the VMAT-1/VMAT-2 inhibitor reserpine but not by the VMAT-2 inhibitor tetrabenazine. Finally, we demonstrated that tubular VMAT-1 mRNA and protein expression were significantly upregulated during a high-sodium diet. In conclusion, our results show for the first time the expression of a VMAT in the renal proximal tubule and its involvement in regulation of dopamine secretion. These data represent the first step toward the comprehension of the role of this transporter in renal dopamine handling and its involvement in pathological situations.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale U858 and Université Toulouse III, Institut de Médecine Moléculaire de Rangueil, Toulouse, France.
authors.forename: Agnès
authors.initials: A
authors.lastname: Maurel
authors.forename: Odile
authors.initials: O
authors.lastname: Spreux-Varoquaux
authors.forename: Francesco
authors.initials: F
authors.lastname: Amenta
authors.forename: Seyed Khosrow
authors.initials: SK
authors.lastname: Tayebati
authors.forename: Daniele
authors.initials: D
authors.lastname: Tomassoni
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Seguelas
authors.forename: Angelo
authors.initials: A
authors.lastname: Parini
authors.forename: Nathalie
authors.initials: N
authors.lastname: Pizzinat
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D004298:Dopamine / Q000096:biosynthesis / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D007150:Immunohistochemistry; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017673:Sodium Chloride, Dietary / Q000008:administration & dosage / Q000494:pharmacology; D050493:Vesicular Monoamine Transport Proteins / Q000378:metabolism / Q000502:physiology*
chemical_list: D017673:Sodium Chloride, Dietary; D050493:Vesicular Monoamine Transport Proteins; D004298:Dopamine