title: [Effects of butyrylchitosan on expression of proliferating cell nuclear antigen in fibroblast proliferation of rabbit eyes after filtering operation].
abstract: OBJECTIVE
  To investigate the effect of butyrylchitosan on the expression of proliferating cell nuclear antigen ( PCNA) in fibroblast proliferation of rabbit eyes after filtering operation.
  
  
  METHODS
  Twenty-four New Zealand rabbits were randomly divided into 2 groups, with 12 rabbits in each group. Rabbits in one group received butyrylchitosan under scleral patch of trabeculectomy in right eyes and trabeculectomy in left eyes (trabeculectomy group). Rabbits in the other group received mitomycin C (MMC) in trabeculectomy in right eyes (MMC group) and without operation in left eyes. Rabbits were killed 1, 4, and 12 weeks after operations. Immunohistochemical staining was used to detected PCNA expression in fibroblast.
  
  
  RESULTS
  After use of butyrylchitosan, the PCNA expression significantly decreased compared with trabeculectomy group (P < 0. 001). PCNA expression in MMC group was significantly lower than in trabeculectomy group (P <0. 001).
  
  
  CONCLUSIONS
  Using butyrylchitosan under scleral patch of trabeculectomy decreases PCNA expression in proliferating cell and inhibits the scarring at filtering site.
authors.affiliation: Department of Ophthalmology, the First People's Hospital of Lanzhou City, Lanzhou 730050, China. weiping966@163.com
authors.forename: Ping
authors.initials: P
authors.lastname: Wei
authors.forename: Ai-qin
authors.initials: AQ
authors.lastname: Wang
authors.forename: Xun-wen
authors.initials: XW
authors.lastname: Lei
authors.forename: Zhi-xin
authors.initials: ZX
authors.lastname: Cao
authors.forename: Xiao-lin
authors.initials: XL
authors.lastname: Li
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000031:analogs & derivatives / Q000494:pharmacology; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D018463:Filtering Surgery; D008297:Male; D008567:Membranes, Artificial; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis*; D011817:Rabbits
chemical_list: D008567:Membranes, Artificial; D018809:Proliferating Cell Nuclear Antigen; D048271:Chitosan