title: Paradoxical effects of substitution and deletion mutation of Arg56 on the structure and chaperone function of human alphaB-crystallin.
abstract: Human alphaB-crystallin is a small heat-shock protein that functions as a molecular chaperone. Recent studies indicate that deletion of a peptide (54FLRAPSWF61) from its N-terminus makes it a better chaperone, and this particular sequence is thought to participate in substrate interaction and subunit exchange with alphaA-crystallin. To determine whether the positive charge on arginine 56 (R56) influences these functions, we prepared human alphaB-crystallin mutants in which R56 was deleted (DeltaR56) or replaced by alanine (R56A). To determine if the effects are specific to R56, we generated two additional mutant proteins in which the two neighboring amino acids were deleted (DeltaL55 and DeltaA57). Dynamic light scattering studies suggested that none of the mutations affected the oligomeric mass of the protein. Far-ultraviolet circular dichroism (UV CD) spectra revealed greater helicity in the secondary structures of R56A and DeltaR56 compared to that of the wild-type (Wt) protein. Near-UV CD spectra showed that the tertiary structure is perturbed in all mutants. Insulin and citrate synthase aggregation assays showed 38 and 30% improvement of chaperone function in DeltaR56 compared to that of the Wt. In contrast, the R56A mutant lost most of its chaperone function. Deletion mutants, DeltaL55 and DeltaA57, showed no significant changes in the chaperone function compared to that of the Wt. The DeltaR56 mutant had a higher surface hydrophobicity than the Wt, but the R56A mutant had a lower hydrophobicity. Our data show paradoxical effects of the deletion and substitution of R56 and imply that the chaperone function of human alphaB-crystallin is dictated not only by the positive charge on R56 but also by the conformational change that it bestows on the protein.
authors.affiliation: Department of Ophthalmology, Case Western Reserve University, Cleveland, Ohio 44106, USA.
authors.forename: Ashis
authors.initials: A
authors.lastname: Biswas
authors.forename: Jeffery
authors.initials: J
authors.lastname: Goshe
authors.forename: Antonia
authors.initials: A
authors.lastname: Miller
authors.forename: Puttur
authors.initials: P
authors.lastname: Santhoshkumar
authors.forename: Carol
authors.initials: C
authors.lastname: Luckey
authors.forename: Manjunatha B
authors.initials: MB
authors.lastname: Bhat
authors.forename: Ram H
authors.initials: RH
authors.lastname: Nagaraj
mesh_terms: D019943:Amino Acid Substitution; D001120:Arginine / Q000235:genetics*; D002950:Citrate (si)-Synthase / Q000378:metabolism; D019281:Dimerization; D016368:Frameshift Mutation; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007328:Insulin / Q000378:metabolism; D018832:Molecular Chaperones / Q000502:physiology; D009154:Mutation; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D038203:alpha-Crystallin B Chain / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism
chemical_list: D007328:Insulin; D018832:Molecular Chaperones; D038203:alpha-Crystallin B Chain; D001120:Arginine; D002950:Citrate (si)-Synthase