Biodegradation of methylthio-s-triazines by Rhodococcus sp. strain FJ1117YT, and production of the corresponding methylsulfinyl, methylsulfonyl and hydroxy analogues.
A novel bacterial strain FJ1117YT was isolated from an enrichment culture with the herbicide simetryn. The isolate was capable of degrading the herbicide supplied as the sole sulfur source in an aquatic batch culture. The strain FJ1117YT was identified as that belonging to Rhodococcus sp. on the basis of comparative morphology, physiological characteristics and comparison of the 16S rRNA gene sequence. The biodegradation pathway of simetryn was established by isolating the methylsulfinyl analogue as the first metabolite and by identification of the methylsulfonyl intermediate and the hydroxy analogue by liquid chromatography-mass spectrometry (LC-MS) and/or nuclear magnetic resonance (NMR) analysis. The results indicate that the methylthio group was progressively oxidised and hydrolysed by the strain FJ1117YT. The same strain is also able to metabolise other methylthio-s-triazines such as ametryn, desmetryn, dimethametryn and prometryn through similar pathways.
Fujii
Kunihiko
K
Kowa Research Institute, Kowa Co., Ltd., 1-25-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
Takagi
Kazuhiro
K
Hiradate
Syuntaro
S
Iwasaki
Akio
A
Harada
Naoki
N
D001673:Biodegradation, Environmental; D002853:Chromatography, Liquid; D006540:Herbicides / Q000737:chemistry / Q000378:metabolism*; D007477:Ions / Q000737:chemistry; D013058:Mass Spectrometry; D019906:Nuclear Magnetic Resonance, Biomolecular; D010084:Oxidation-Reduction; D011400:Prometryne / Q000737:chemistry / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000145:classification / Q000235:genetics; D012240:Rhodococcus / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D013431:Sulfates / Q000737:chemistry; D014227:Triazines / Q000737:chemistry / Q000378:metabolism*; D014815:Vitamins / Q000737:chemistry
D006540:Herbicides; D007477:Ions; D012336:RNA, Ribosomal, 16S; D013431:Sulfates; D014227:Triazines; D014815:Vitamins; C033807:simetryn; D011400:Prometryne; C100057:ametryne; C077544:dimethametryn; C009232:semeron