title: Clonal Multiplication of Aconitum carmichaeli by Tip Tissue Culture and Alkaloid Contents of Clonally Propagated Plant.
abstract: Shoot tips and axillary buds of ACONITUM CARMICHAELI Debx. cultured in Murashige and Skoog medium supplemented with 5 ppm BAP at 25 +/- 1 degrees C under continuous light for 6 weeks formed multiple shoots with an average of 7 shoots per culture. Multiple shoot forming ability does not decrease at least for 6 generations. Subsequent culture of these shoots on MS medium supplemented with 0.5 ppm IAA at 20 +/- 1 degrees C under continuous light for 6 weeks resulted in root formation. The plantlets were transplanted successfully in the soil. Alkaloid contents of clonally-propagated plants showed less variation than those of the normally grown plants.
authors.affiliation: Faculty of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hatano
authors.forename: K
authors.initials: K
authors.lastname: Kamura
authors.forename: Y
authors.initials: Y
authors.lastname: Shoyama
authors.forename: I
authors.initials: I
authors.lastname: Nishioka