Sergeia podlipaevi gen. nov., sp. nov. (Trypanosomatidae, Kinetoplastida), a parasite of biting midges (Ceratopogonidae, Diptera).
Three strains of a trypanosomatid protozoan were isolated from the midguts of two naturally infected species of biting midges [Culicoides (Oecacta) festivipennis and Culicoides (Oecacta) truncorum] and characterized by light and electron microscopy and by molecular techniques. Morphological characteristics and sequences of the 18S rRNA, 5S rRNA, spliced leader RNA and glycosomal glyceraldehyde-3-phosphate dehydrogenase genes indicate that the studied flagellates represent a novel phylogenetic lineage within the Trypanosomatidae. Based on phylogenetic analyses, the novel endosymbiont-free, monoxenous trypanosomatid was classified as Sergeia podlipaevi gen. nov., sp. nov. Interestingly, it is closely related to another trypanosomatid species that parasitizes the sand fly Lutzomyia evansi, a blood-sucking dipteran from South America. The type strain of S. podlipaevi sp. nov., ICUL/CZ/2000/CER3, was obtained from Malpighian tubes. Of 2518 females of seven species of biting midges trapped in the Czech Republic, more than 1.5 % were infected by trypanosomatid parasites. An unrelated insect species, Culicoides (Monoculicoides) nubeculosus, was experimentally infected with S. podlipaevi, demonstrating that its host range extends to different subgenera of biting midges.
Svobodová
Milena
M
Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic.
Zídková
Lenka
L
Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic.
Čepička
Ivan
I
Department of Zoology, Faculty of Science, Charles University, Prague, Czech Republic.
Oborník
Miroslav
M
Biology Centre, Institute of Parasitology, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic.
Lukeš
Julius
J
Faculty of Biology, University of South Bohemia, České Budějovice (Budweis), Czech Republic.|Biology Centre, Institute of Parasitology, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic.
Votýpka
Jan
J
Biology Centre, Institute of Parasitology, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic.|Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic.
D000818:Animals; D002519:Ceratopogonidae / Q000166:cytology / Q000469:parasitology* / Q000648:ultrastructure; D018105:DNA, Kinetoplast / Q000032:analysis; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D041981:Gastrointestinal Tract / Q000469:parasitology; D020459:Genes, rRNA; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / Q000235:genetics; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010802:Phylogeny; D016053:RNA, Protozoan / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014351:Trypanosomatina / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification*
D018105:DNA, Kinetoplast; D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D016053:RNA, Protozoan; D012337:RNA, Ribosomal, 18S; D012341:RNA, Ribosomal, 5S; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)