[Preparation of porous polylactic-acid/ bone matrix gelatin composite as scaffold materials for bone-tissue engineering].
OBJECTIVE
To develop a novel porous poly lactic-acid(PLA)/bone matrix gelatin (BMG) bioactive composite biomaterial with supercritical carbon dioxide (SC-CO2) extraction and determine the optimal proportion of the ingredients by evaluating the physical and chemical characteristics and cellular compatibility of the composites.


METHODS
The PLA/BMG composite biomaterials with various PLA/BMG proportions were prepared with SC-CO2 technique and evaluated by macroscopic observation, porosity and mechanical assessment, and scanning electron micrograph (SEM). The optimal proportion of PLA/BMG was determined in conjunction with the results of cellular compatibility evaluation.


RESULTS
BMG proportion in BLA/BMG composite biomaterials prepared with SC-CO2 technique was positively associated with the cellular compatibility and porosity, and inversely with the mechanics of the prepared composite. The results showed BMG content of 30% in PLA/BMG composite was optimal.


CONCLUSIONS
The composite with PLA and BMG proportion of 7:3 possesses good physical and chemical characteristics and cellular compatibility, suggesting its potential as the bone implant biomaterial and bone-tissue engineering scaffold.
Zhang
Yu-min
YM
Research Center of Tissue Engineering, Southern Medical University, Guangzhou 510515, China. zymsir@sina.com
Li
Bao-xing
BX
Li
Ji
J
Ma
Hong-qiang
HQ
Zhao
Ya-ping
YP
Yuan
Lin
L
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001857:Bone Matrix / Q000737:chemistry; D018786:Bone Substitutes / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D051381:Rats; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; D018786:Bone Substitutes; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D005780:Gelatin