Heat-shock proteins modulate the incidence of apoptosis and oxidative stress in preimplantation mouse embryos.
We investigated the effect of antibodies to heat-shock proteins 60 and 70 on development, reactive oxygen species production, and incidence of apoptosis in preimplantation mouse embryos. Supplementation of culture media with antibodies to heat-shock proteins 60 and 70 resulted in significantly lower blastocyst development, hatching rate, reduced total cell number, and trophectoderm cell number, and increased the incidence of apoptosis in blastocysts compared to the control group.
Esfandiari
Navid
N
Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Department of Obstetrics Gynecology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA.
Falcone
Tommaso
T
Goldberg
Jeffrey M
JM
Agarwal
Ashok
A
Sharma
Rakesh K
RK
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001755:Blastocyst / Q000166:cytology* / Q000378:metabolism / Q000502:physiology*; D046149:Embryo Culture Techniques / Q000379:methods; D047108:Embryonic Development / Q000502:physiology; D005260:Female; D006360:Heat-Shock Proteins / Q000502:physiology*; D051379:Mice; D018384:Oxidative Stress / Q000502:physiology*; D011247:Pregnancy; D017382:Reactive Oxygen Species / Q000378:metabolism
D006360:Heat-Shock Proteins; D017382:Reactive Oxygen Species