title: MPSS profiling of embryonic gonad and primordial germ cells in chicken.
abstract: The massively parallel signature sequencing (MPSS) provides a greater depth of coverage than expressed sequence tag scan or microarray and provides a comprehensive expression profile. We used the MPSS technology to uncover gene expression profiling in the early embryonic gonads and primordial germ cells (PGCs) in the chicken. Total numbers of sequenced signatures were 1,012,533 and 995,676 for the PGCs and gonad, respectively. Using a noise distribution model, we found that 1.67% of all signatures are expressed at a higher level in PCGs and 2.81% of all signatures are expressed at a higher level in the gonad. The MPSS data are presented via an interactive web interface available at http://snugenome.snu.ac.kr/MPSS. The MPSS data have been submitted to the Gene Expression Omnibus of the National Center for Biotechnology Information (accession number GSM137300 and GSM137301 for PGCs and gonad, respectively).
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul, Korea.
authors.forename: Heebal
authors.initials: H
authors.lastname: Kim
authors.forename: Tae Sub
authors.initials: TS
authors.lastname: Park
authors.forename: Woon Kyu
authors.initials: WK
authors.lastname: Lee
authors.forename: Sunjin
authors.initials: S
authors.lastname: Moon
authors.forename: Jin Nam
authors.initials: JN
authors.lastname: Kim
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Shin
authors.forename: Jin Gyoung
authors.initials: JG
authors.lastname: Jung
authors.forename: Seon Duk
authors.initials: SD
authors.lastname: Lee
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Park
authors.forename: Kyung Je
authors.initials: KJ
authors.lastname: Park
authors.forename: Mi A
authors.initials: MA
authors.lastname: Kim
authors.forename: Sang Su
authors.initials: SS
authors.lastname: Shin
authors.forename: Tae Min
authors.initials: TM
authors.lastname: Kim
authors.forename: Jungrye
authors.initials: J
authors.lastname: Nam
authors.forename: Yeonkyung
authors.initials: Y
authors.lastname: Kang
authors.forename: Jeong Mook
authors.initials: JM
authors.lastname: Lim
authors.forename: Jae Yong
authors.initials: JY
authors.lastname: Han
mesh_terms: D017398:Alternative Splicing / Q000235:genetics; D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D002874:Chromosome Mapping; D030541:Databases, Genetic; D020869:Gene Expression Profiling / Q000379:methods*; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D005854:Germ Cells / Q000378:metabolism*; D006066:Gonads / Q000196:embryology* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D012333:RNA, Messenger