Probiotic effects on 5-fluorouracil-induced mucositis assessed by the sucrose breath test in rats.
The sucrose breath test (SBT) was employed to noninvasively assess the efficacy of probiotics in 5-fluorouracil (5-FU)-induced intestinal mucositis. Dark Agouti rats were allocated to 5 groups (n = 10): 5-FU + L. fermentum BR 11, 5-FU + L. rhamnosus GG, 5-FU + B. lactis BB 12, 5-FU + skim milk (SM), and saline + SM. Probiotics were administered by oral gavage for 10 days. Mucositis was induced on day 7 by intraperitoneal injection of 5-FU (150 mg/kg) or vehicle (saline). Rats were sacrificed 72 h after 5-FU injection. The SBT measured breath 13CO2 (expressed as percentage cumulative dose at 90 min; %CD90) on days 0, 7, and 10. %CD90 was significantly lower in 5-FU-treated controls compared with that in saline-treated controls on day 10. 5-FU caused an 83% reduction in sucrase and a 510% increase in MPO activity. The SBT detected damage induced by 5-FU and is a simple, noninvasive indicator of small bowel injury. The probiotics assessed offered no protection from mucositis at the dose tested.
Mauger
Chad A
CA
Centre for Paediatric and Adolescent Gastroenterology, Children, Youth and Women's Health Service, 72 King William Road, North Adelaide, South Australia, Australia.
Butler
Ross N
RN
Geier
Mark S
MS
Tooley
Katie L
KL
Howarth
Gordon S
GS
D000818:Animals; D001644:Bifidobacterium; D001944:Breath Tests; D004195:Disease Models, Animal; D005260:Female; D005472:Fluorouracil / Q000009:adverse effects; D007082:Ileum / Q000378:metabolism; D007166:Immunosuppressive Agents / Q000009:adverse effects; D007583:Jejunum / Q000473:pathology; D052197:Limosilactobacillus fermentum; D052201:Lacticaseibacillus rhamnosus; D009195:Peroxidase / Q000378:metabolism; D019936:Probiotics / Q000627:therapeutic use*; D051381:Rats; D011919:Rats, Inbred Strains; D013280:Stomatitis / Q000139:chemically induced / Q000175:diagnosis / Q000628:therapy*; D013393:Sucrase / Q000378:metabolism
D007166:Immunosuppressive Agents; D009195:Peroxidase; D013393:Sucrase; D005472:Fluorouracil