Microscopic detection of thermogenesis in a single HeLa cell.
We report here the technique for detection and measurement of the temperature changes in single cells using a recently devised microthermometer (a glass micropipette filled with the thermosensitive fluorescent dye Europium (III) thenoyltrifluoroacetonate trihydrate). We found that the heat production in a single HeLa cell occurred with some time delay after the ionomycin-induced Ca(2+) influx from the extracellular space. The time delay inversely depended on extracellular [Ca(2+)], and the increase in temperature was suppressed when Ca(2+)-ATPases were blocked by thapsigargin. These observations strongly suggest that the enzymatic activity of Ca(2+)-ATPases in endoplasmic reticulum leads to the heat production. This study has therefore paved the way for studying the thermogenesis at the single-cell level.
Suzuki
Madoka
M
Tseeb
Vadim
V
Oyama
Kotaro
K
Ishiwata
Shin'ichi
S
D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*; D022722:Thermogenesis / Q000502:physiology*; D013817:Thermography / Q000295:instrumentation / Q000379:methods*