Immunohistolocalization and gene expression of the secretory carbonic anhydrase isozymes (CA-VI) in canine oral mucosa, salivary glands and oesophagus.
The immunohistolocalization of secretory carbonic anhydrase isoenzymes (CA-VI) in canine salivary glands, parotid, submandibular, sublingual and zygomatic glands, oral and oesophageal mucosa was studied using a specific antiserum against a canine CA-VI. In addition, the gene expression of CA-VI from the same tissue was studied using a real-time reverse-transcriptase polymerase chain reaction. In all salivary glands and oesophageal gland, immunostaining intensely localized CA-VI antiserum throughout the cytoplasm of serous acinar cells, including serous demilune and ductal epithelial cells. In contrast, no immunoreaction localized CA-VI in the mucous acinar cells of the gland. CA-VI gene transcripts were also detected in the same areas. The physiological significance of secretory CA-VI in the oral and oesophageal cavity is thought to play a highly specialized role in the maintenance of bicarbonate level in saliva and to protect mucosa from acid injury. It is shown that the major sites of the CA-VI secretion in dogs were in serous (demilune) secretory cells in all four major salivary glands and oesophageal glands in particular.
Kasuya
T
T
Laboratory of Anatomy I, Azabu University School of Veterinary Medicine, Fuchinobe 1-17-71, Sagamihara, Kanagawa, 229-8501, Japan.
Shibata
S
S
Kaseda
M
M
Ichihara
N
N
Nishita
T
T
Murakami
M
M
Asari
M
M
D000818:Animals; D002256:Carbonic Anhydrases / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D004285:Dogs; D004947:Esophagus / Q000201:enzymology*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D007150:Immunohistochemistry / Q000379:methods / Q000662:veterinary*; D008297:Male; D009061:Mouth Mucosa / Q000201:enzymology*; D021122:Protein Subunits / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D012469:Salivary Glands / Q000201:enzymology*
D021122:Protein Subunits; D002256:Carbonic Anhydrases; C477051:carbonic anhydrase VI