[Changes of apoptosis, mitochondrion membrane potential and Ca2+ of hypothalamic neurons induced by high power microwave].
OBJECTIVE
To explore the injury effect and mechanism of hypothalamic neurons after high power microwave (HPM) exposure.


METHODS
Primarily cultured hypothalamic neurons were exposed to 10 and 30 mW/cm(2) HPM, and the inverted phase contrast microscope (IPCM) and flow cytometry (FCM) were employed to detect the injury of cells and change of mitochondrion membrane potential (MMP) and Ca(2+) in the cytoplasm of neurons.


RESULTS
The ratio of apoptosis was significantly higher than that of the sham exposure (P < 0.05) induced by 10 and 30 mW/cm(2) HPM and necrosis increased significantly (P < 0.05) in the group of 30 mW/cm(2) at 6 h after exposure. The content of Ca(2+) in the cytoplasm of neuron cells increased (P < 0.01) while MMP decreased significantly (P < 0.01) after radiation of 30 mW/cm(2) HPM at 6 h after exposure.


CONCLUSIONS
Apoptosis is one of the major death ways of hypothalamic neurons. The overloading of Ca(2+) and the decline of MMP are involved in the process.
Meng
Li
L
Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
Peng
Rui-Yun
RY
Gao
Ya-Bing
YB
Wang
Shui-Ming
SM
Ma
Jun-Jie
JJ
Hu
Wen-Hua
WH
Wang
De-Wen
DW
Su
Zhen-Tao
ZT
Dong
Bo
B
Xu
Tian-Hao
TH
D000818:Animals; D017209:Apoptosis / Q000528:radiation effects*; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D007031:Hypothalamus / Q000166:cytology / Q000528:radiation effects; D053078:Membrane Potential, Mitochondrial / Q000528:radiation effects*; D008564:Membrane Potentials; D008872:Microwaves / Q000009:adverse effects*; D009474:Neurons / Q000378:metabolism* / Q000528:radiation effects*; D051381:Rats; D017208:Rats, Wistar
D002118:Calcium