Sera from mice immunized with DNA encoding Porphyromonas gingivalis catalytic or adhesin part of HRgpA inhibit degradation of human fibronectin by HRgpA.
Gingipains are potent virulence factors of Porphyromonas gingivalis and are likely to be associated with the development of periodontitis. It is, therefore, suggested that gingipain inhibition by vaccination could be a useful therapy for adult periodontitis. This study investigated the ability of antibodies raised against the catalytic part and the adhesin/haemagglutinin part of HRgpA to prevent haemagglutination and fibronectin degradation caused by P. gingivalis. We constructed two DNA vaccines, one containing the adhesin part of HRgpA and one with the catalytic part of HRgpA. BALB/c mice were immunized intramuscularly with either catalytic-part-encoding plasmids, adhesin-part-encoding plasmids or empty control plasmids. Sera from mice immunized with the catalytic vaccine or the adhesin vaccine each showed inhibition of human fibronectin degradation. A DNA vaccine encoding the adhesin or catalytic part of HRgpA induces responses that inhibit the degradation of molecules important for the structure and function of gingival and bone tissues.
Vågnes
K S
KS
Department of Oral Sciences--Oral Microbiology, Bergen, Norway. kari.vagnes@odont.uib.no
Vågnes
O
O
Bakken
V
V
D018829:Adhesins, Bacterial / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D004269:DNA, Bacterial / Q000627:therapeutic use*; D005353:Fibronectins / Q000378:metabolism*; D018014:Gene Transfer Techniques; D000080867:Gingipain Cysteine Endopeptidases; D006388:Hemagglutinins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007114:Immunization / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis; D007132:Immunoglobulin Isotypes / Q000032:analysis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018482:Muscle, Skeletal / Q000201:enzymology; D010447:Peptide Hydrolases / Q000378:metabolism; D016966:Porphyromonas gingivalis / Q000201:enzymology* / Q000235:genetics
D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D004269:DNA, Bacterial; D005353:Fibronectins; D000080867:Gingipain Cysteine Endopeptidases; D006388:Hemagglutinins; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D010447:Peptide Hydrolases; D003546:Cysteine Endopeptidases