title: Laser burn wound healing in naso-labial region of fetal and neonatal mice.
abstract: OBJECTIVE
  To investigate the characteristics of wound healing in the mouse naso-labial region in both the fetal and neonatal stages, histological and immunohistochemical analyses were performed using a newly established laser burn wound healing system.
  
  
  METHODS
  Fetal mice at embryonic day 14 (E 14) were wounded as a model of fetal wound healing. To compare it, neonatal mice at day 5 after birth (d 5) were adopted as a model of neonatal wound healing. The healing process was examined by van Gieson staining and immunohistochemistry for fibronectin and tenascin.
  
  
  RESULTS
  Relatively large damage remained after wound healing even in fetal mice. In both types of wound healing, rapid regeneration of muscle tissues were observed. Fibronectin and tenascin immunostaining was detected not only in wound healing region, but also in the endomysium of regenerating muscle tissues. Especially, tenascin showed a restricted expression pattern.
  
  
  CONCLUSIONS
  Rapid regeneration of muscle tissues in the naso-labial region in both the fetal and neonatal mice seemed to leave relatively large damage even in the fetal wound healing. Contracted force exerted by muscle tissues may be a reason for this phenomenon. Fibronectin and tenascin were closely related to the wound healing process including muscle regeneration in this region.
authors.affiliation: Maxillofacial Orthognathics, Department of Maxillofacial Reconstruction and Function, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan. a-nakasone.mort@tmd.ac.jp
authors.forename: A
authors.initials: A
authors.lastname: Nakasone
authors.forename: S
authors.initials: S
authors.lastname: Shibata
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
authors.forename: Y
authors.initials: Y
authors.lastname: Yamashita
authors.forename: K
authors.initials: K
authors.lastname: Ohyama
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D004396:Coloring Agents; D004195:Disease Models, Animal; D016326:Extracellular Matrix Proteins / Q000032:analysis; D005152:Facial Muscles / Q000196:embryology / Q000293:injuries / Q000503:physiopathology; D005260:Female; D005333:Fetus; D005353:Fibronectins / Q000032:analysis; D005865:Gestational Age; D007834:Lasers / Q000009:adverse effects*; D008046:Lip / Q000196:embryology / Q000293:injuries* / Q000503:physiopathology; D051379:Mice; D008813:Mice, Inbred ICR; D009666:Nose / Q000196:embryology / Q000293:injuries* / Q000503:physiopathology; D011247:Pregnancy; D049188:Prenatal Injuries / Q000503:physiopathology*; D012038:Regeneration / Q000502:physiology; D019063:Tenascin / Q000032:analysis; D014945:Wound Healing / Q000502:physiology
chemical_list: D004396:Coloring Agents; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D019063:Tenascin