Identification of Beijing lineage Mycobacterium tuberculosis with combined mycobacterial interspersed repetitive unit loci 26, 31, and ETR-A.
A rapid method for identification of Beijing lineage Mycobacterium tuberculosis is still needed in regions of tuberculosis endemicity, especially if genotyping methods are not readily accessible. After analyzing 1,557 clinical isolates, a PCR method with combined mycobacterial interspersed repetitive unit loci 26, 31, and ETR-A for differentiation of Beijing lineage isolates was established, the sensitivity and specificity of which are 94.7% and 98.5%, respectively.
Chin
Pei-Ju
PJ
Reference Laboratory of Mycobacteriology, Research and Diagnostic Center, Centers for Disease Control, Department of Health, 161 Kun-Yang St., Nan-Kang, Taipei 115, Taiwan, Republic of China.
Chiu
Chen-Che
CC
Jou
Ruwen
R
D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques / Q000379:methods*; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D005838:Genotype; D006801:Humans; D020071:Interspersed Repetitive Sequences / Q000235:genetics*; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics; D020080:Tandem Repeat Sequences; D014157:Transcription Factors / Q000235:genetics*; D014376:Tuberculosis / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000382:microbiology
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D014157:Transcription Factors