Measurement of bacterial gene expression in vivo by laser capture microdissection and quantitative real-time RT-PCR.
Due to the relative small number of bacterial pathogens present in an infected host, exploration of pathogen gene expression in vivo is challenging. This study reports the development of a protocol for quantifying bacterial gene expression in vivo in Actinobacillus pleuropneumoniae using laser capture microdissection and real-time quantitative RT-PCR.
Klitgaard
Kirstine
K
National Veterinary Institute, Technical University of Denmark, Bülowsvej 27, DK-1790 Copenhagen V, Denmark.
Jensen
Tim K
TK
Angen
Øystein
Ø
Boye
Mette
M
D000189:Actinobacillus Infections / Q000382:microbiology*; D016977:Actinobacillus pleuropneumoniae / Q000235:genetics* / Q000302:isolation & purification*; D000818:Animals; D015964:Gene Expression Regulation, Bacterial; D008168:Lung / Q000382:microbiology; D042282:Microdissection / Q000379:methods*; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D013552:Swine
D012329:RNA, Bacterial