title: Tyrosine phosphorylation and lipid raft association of pseudorabies virus glycoprotein E during antibody-mediated capping.
abstract: In specific cell types infected with the alphaherpesviruses herpes simplex virus and pseudorabies virus (PRV), addition of virus-specific antibodies results in redistribution of cell-surface-anchored viral proteins. This redistribution is triggered by the viral protein gE and consists of the directional movement of the antibody-antigen complexes to one pole of the cell. This viral capping process has been associated with increased antibody-resistant virus spread and strongly resembles immunoreceptor capping, a process that is crucial in activation of different immune cells (e.g. capping of Fcgamma-receptors, B and T cell receptors). Here, we report that the PRV gE-mediated viral capping process results in increased Src kinase-mediated tyrosine phosphorylation of the cytoplasmic domain of gE and that a fraction of gE associates with lipid rafts, all very reminiscent of immunoreceptor capping. These results provide evidence that gE-mediated capping is a viral mimicry of immunoreceptor capping.
authors.affiliation: Department of Virology, Parasitology and Immunology, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium. Ann.Desplanques@UGent.be
authors.forename: Ann S
authors.initials: AS
authors.lastname: Desplanques
authors.forename: Hans J
authors.initials: HJ
authors.lastname: Nauwynck
authors.forename: Kelly
authors.initials: K
authors.lastname: Tilleman
authors.forename: Dieter
authors.initials: D
authors.lastname: Deforce
authors.forename: Herman W
authors.initials: HW
authors.lastname: Favoreel
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D000914:Antibodies, Viral / Q000378:metabolism*; D002460:Cell Line; D011558:Herpesvirus 1, Suid / Q000276:immunology / Q000378:metabolism*; D007152:Immunologic Capping; D021962:Membrane Microdomains / Q000737:chemistry*; D018613:Microscopy, Confocal; D010766:Phosphorylation; D017434:Protein Structure, Tertiary; D013552:Swine; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D019061:src-Family Kinases / Q000378:metabolism
chemical_list: D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; C089580:glycoprotein E, Suid herpesvirus 1; D019061:src-Family Kinases