title: Microfluidic library screening for mapping antibody epitopes.
abstract: The capability to screen molecular libraries using disposable microfluidic devices provides the potential to simplify and automate reagent generation and to develop integrated bioanalytical systems for clinical diagnostics. Here, antibody epitopes were mapped using a disposable microfluidic device to screen a combinatorial peptide library composed of 5 x 108 members displayed on bacterial cells. On-chip library screening was achieved in a two-stage, continuous-flow microfluidic sorter that separates antibody-binding target cells captured on microspheres through dielectrophoretic funneling. The antibody fingerprints identified were comparable to those obtained using state-of-the-art commercial cell sorting instrumentation.
authors.affiliation: Department of Chemical Engineering, and Institute for Collaborative Biotechnologies, University of California, Santa Barbara, California 93106, USA.
authors.forename: Paul H
authors.initials: PH
authors.lastname: Bessette
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Hu
authors.forename: Hyongsok T
authors.initials: HT
authors.lastname: Soh
authors.forename: Patrick S
authors.initials: PS
authors.lastname: Daugherty
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D018604:Epitope Mapping / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008969:Molecular Sequence Data; D019151:Peptide Library
chemical_list: D000911:Antibodies, Monoclonal; D019151:Peptide Library