Thermodynamic properties of enzyme-catalyzed reactions involving cytosine, uracil, thymine, and their nucleosides and nucleotides.
The standard Gibbs energies of formation of species in the cytidine triphosphate series, uridine triphosphate series, and thymidine triphosphate series have been calculated on the basis of the convention that Delta(f)G=0 for the neutral form of cytidine in aqueous solution at 298.15 K at zero ionic strength. This makes it possible to calculate apparent equilibrium constants for a number of reactions for which apparent equilibrium constants have not been measured or cannot be measured because they are too large. This paper adds fifteen reactants to the database BasicBiochemData3 at MathSource that includes 199 reactants. The standard transformed Gibbs energies of formation of these fifteen reactants are used to calculate apparent equilibrium constants at 298.15 K, ionic strength 0.25 M, and pHs 5, 6, 7, 8, and 9 for thirty two reactions. The pKs, standard Gibbs energies of hydrolysis, and standard Gibbs energies of deamination are given for these fifteen reactants.
Alberty
Robert A
RA
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, United States. alberty@mit.edu
D002384:Catalysis; D003596:Cytosine / Q000737:chemistry; D004798:Enzymes / Q000737:chemistry*; D006868:Hydrolysis; D008956:Models, Chemical; D011741:Pyrimidine Nucleosides / Q000737:chemistry*; D011742:Pyrimidine Nucleotides / Q000737:chemistry*; D011744:Pyrimidinones / Q000737:chemistry*; D013816:Thermodynamics; D013941:Thymine / Q000737:chemistry; D014498:Uracil / Q000737:chemistry
D004798:Enzymes; D011741:Pyrimidine Nucleosides; D011742:Pyrimidine Nucleotides; D011744:Pyrimidinones; D014498:Uracil; D003596:Cytosine; D013941:Thymine