title: Influence of plant matrix on microwave-assisted extraction process. The case of diosgenin extracted from fenugreek (Trigonella foenum-graecum L.).
abstract: A focused microwave-assisted extraction method was developed for the extraction of diosgenin from fenugreek (Trigonella foenum-graecum) seeds, air-dried and fresh leaves and air-dried roots. Several experimental parameters were studied, including extraction time, microwave power applied and percentage of 2-propanol in the extraction mixture as well as their interactions, in order to optimize the extraction efficiency. The two latter parameters were found to be the most important. Response surface modelling was used to predict the extraction yield of diosgenin in selected matrices. The analysis of diosgenin in crude extracts was carried out by capillary gas chromatography-mass spectrometry (GC-MS).
authors.affiliation: Laboratory of Pharmaceutical Analytical Chemistry, School of Pharmaceutical Sciences EPGL, University of Geneva, 20 bd d'Yvoy, CH-1211 Geneva 4, Switzerland.
authors.forename: Béatrice
authors.initials: B
authors.lastname: Kaufmann
authors.forename: Serge
authors.initials: S
authors.lastname: Rudaz
authors.forename: Samir
authors.initials: S
authors.lastname: Cherkaoui
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Veuthey
authors.forename: Philippe
authors.initials: P
authors.lastname: Christen
mesh_terms: D000465:Algorithms; D004144:Diosgenin / Q000737:chemistry* / Q000302:isolation & purification; D008401:Gas Chromatography-Mass Spectrometry; D008872:Microwaves; D010936:Plant Extracts / Q000032:analysis; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry; D029910:Trigonella / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D004144:Diosgenin