title: Study of the virulence and cross-neutralization capability of recent porcine parvovirus field isolates and vaccine viruses in experimentally infected pregnant gilts.
abstract: The pathogenicity of two recent German field isolates of Porcine parvovirus (PPV-27a and PPV-143a) and two vaccine viruses [PPV-NADL-2 and PPV-IDT (MSV)], which are used for the production of inactivated vaccines, was investigated by inoculation of pregnant sows at day 40 of gestation. Post-infection sera of these sows as well as antisera prepared in rabbits by immunization with the four above-mentioned PPV isolates and with the virulent strain PPV-Challenge (Engl.) were tested for their homologous and heterologous neutralization activities. All antisera had high neutralization activity against the vaccine viruses, the PPV-Challenge (Engl.) virus and PPV-143a, but much lower activity against PPV-27a. These results suggest that PPV-27a represents a new antigenic variant or type of PPV and vaccines based on the established vaccine viruses may not be fully protective against this field isolate. PPV-27a has been characterized based on the amino acid sequences of the capsid protein as a member of a new and distinct PPV cluster (Zimmermann et al., 2006). Interestingly, the homologous neutralizing antibody titres of the sera of all three pigs and both rabbits inoculated or immunized with PPV-27a were 100- to 1000-fold lower than the heterologous titres against any of the other viruses. The low homologous neutralizing antibody titres suggest a possible, yet undefined, immune escape mechanism of this PPV isolate.
authors.affiliation: Institute for Animal Hygiene and Veterinary Public Health, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 1, 04103 Leipzig, Germany.
authors.forename: E J L
authors.initials: EJL
authors.lastname: Zeeuw
authors.affiliation: Institute for Animal Hygiene and Veterinary Public Health, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 1, 04103 Leipzig, Germany.
authors.forename: N
authors.initials: N
authors.lastname: Leinecker
authors.affiliation: Impfstoffwerke Dessau-Tornau GmbH, PF 400214, 06855 Rosslau, Germany.
authors.forename: V
authors.initials: V
authors.lastname: Herwig
authors.affiliation: Impfstoffwerke Dessau-Tornau GmbH, PF 400214, 06855 Rosslau, Germany.
authors.forename: H-J
authors.initials: HJ
authors.lastname: Selbitz
authors.affiliation: Institute for Animal Hygiene and Veterinary Public Health, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 1, 04103 Leipzig, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Truyen
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D003429:Cross Reactions; D005260:Female; D009500:Neutralization Tests; D010322:Parvoviridae Infections / Q000276:immunology / Q000503:physiopathology / Q000662:veterinary*; D028323:Parvovirus, Porcine / Q000276:immunology / Q000472:pathogenicity*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000276:immunology / Q000503:physiopathology / Q000662:veterinary* / Q000821:virology; D011817:Rabbits; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000503:physiopathology* / Q000821:virology; D014765:Viral Vaccines / Q000276:immunology*; D014774:Virulence
chemical_list: D000914:Antibodies, Viral; D014765:Viral Vaccines