title: Tyrosine nitration is a novel post-translational modification occurring on the neural intermediate filament protein peripherin.
abstract: The biological implication of protein tyrosine nitration in signaling pathways triggered by nitric oxide is recently emerging. Here we report for the first time that nitrotyrosination occurs in the neural intermediate filament protein peripherin. In neuron-like PC12 cells, nitrated peripherin is associated with the cytoskeleton fraction, its level increases during the progression of NGF-induced differentiation and the nitrated protein remains closely associated with stable microtubules. Tyr 17 and Tyr 376 were identified by MALDI-TOF analyses as two specific residues endogenously nitrated. Finally, peripherin nitration is not restricted to PC12 cells but it is also present in vivo in rat brain.
authors.affiliation: Section of Biochemistry, University of Milano, Via Celoria 10, 20100 Milano, Italy. gabriella.tedeschi@unimi.it
authors.forename: Gabriella
authors.initials: G
authors.lastname: Tedeschi
authors.forename: Graziella
authors.initials: G
authors.lastname: Cappelletti
authors.forename: Simona
authors.initials: S
authors.lastname: Nonnis
authors.forename: Francesca
authors.initials: F
authors.lastname: Taverna
authors.forename: Armando
authors.initials: A
authors.lastname: Negri
authors.forename: Cristina
authors.initials: C
authors.lastname: Ronchi
authors.forename: Severino
authors.initials: S
authors.lastname: Ronchi
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D007381:Intermediate Filament Proteins / Q000737:chemistry* / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000378:metabolism*; D016716:PC12 Cells; D064531:Peripherins; D011499:Protein Processing, Post-Translational; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D000199:Actins; D007381:Intermediate Filament Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D064531:Peripherins; C002744:3-nitrotyrosine; D014443:Tyrosine