title: Trypanosoma cruzi: attachment to perimicrovillar membrane glycoproteins of Rhodnius prolixus.
abstract: Studies were carried out to identify proteins involved in the interface of Trypanosoma cruzi with the perimicrovillar membranes (PMM) of Rhodnius prolixus. Video microscopy experiments demonstrated high level of adhesion of T. cruzi Dm 28c epimastigotes to the surface of posterior midgut cells of non-treated R. prolixus. The parasites however were unable to attach to gut cells obtained from decapitated or azadirachtin-treated insects. The influence of carbohydrates on the adhesion to insect midgut was confirmed by inhibition of parasite attachment after midgut incubation with N-acetylgalactosamine, N-acetylmannosamine, N-acetylglucosamine, D-galactose, D-mannose or sialic acid. We observed that hydrophobic proteins in the surface of epimastigotes bind to polypeptides with 47.7, 45.5, 44, 43, 40.5, 36, 31 and 13kDa from R. prolixus PMM and that pre-incubation of lectins specifically inhibited binding to 31, 40.5, 44 and 45.5kDa proteins. We suggest that glycoproteins from PMM and hydrophobic proteins from epimastigotes are important for the adhesion of the parasite to the posterior midgut cells of the vector.
authors.affiliation: Laboratório de Biologia Molecular e Doenças Endêmicas, Departamento de Bioquímica e Biologia Molecular, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, RJ, Brazil.
authors.forename: C R
authors.initials: CR
authors.lastname: Alves
authors.forename: J M
authors.initials: JM
authors.lastname: Albuquerque-Cunha
authors.forename: C B
authors.initials: CB
authors.lastname: Mello
authors.forename: E S
authors.initials: ES
authors.lastname: Garcia
authors.forename: N F
authors.initials: NF
authors.lastname: Nogueira
authors.forename: S C
authors.initials: SC
authors.lastname: Bourguingnon
authors.forename: W
authors.initials: W
authors.lastname: de Souza
authors.forename: P
authors.initials: P
authors.lastname: Azambuja
authors.forename: M S
authors.initials: MS
authors.lastname: Gonzalez
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002241:Carbohydrates / Q000494:pharmacology; D002448:Cell Adhesion / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007303:Insect Vectors / Q000469:parasitology*; D007306:Insecticides / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000469:parasitology; D036701:Limonins / Q000494:pharmacology; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D018715:Microscopy, Video; D008871:Microvilli / Q000737:chemistry / Q000469:parasitology; D012239:Rhodnius / Q000469:parasitology*; D014349:Trypanosoma cruzi / Q000502:physiology*
chemical_list: D002241:Carbohydrates; D007306:Insecticides; D036701:Limonins; D008562:Membrane Glycoproteins; C010329:azadirachtin