title: Identification and characterization of a human monoclonal antibody that potently neutralizes a broad panel of rabies virus isolates.
abstract: Rabies is a zoonosis that results in millions of human exposures worldwide each year. Human monoclonal antibodies (HuMAbs) that neutralize rabies virus may represent one viable strategy for post-exposure prophylaxis in humans, and have many advantages over current human or equine rabies immune globulin. Transgenic mice carrying human immunoglobulin genes were used to isolate human monoclonal antibodies that neutralized rabies virus. Several HuMAbs were identified that neutralized rabies virus variants from a broad panel of isolates of public health significance. HuMAb 17C7 was the most promising antibody identified because it neutralized all rabies virus isolates tested. HuMAb 17C7 recognizes a conformational epitope on the rabies virus glycoprotein which includes antigenic site III. HuMAb 17C7 protected hamsters from a lethal dose of rabies virus in a well-established in vivo model of post-exposure prophylaxis.
authors.affiliation: Massachusetts Biologic Laboratories, University of Massachusetts Medical School, 305 South Street, Jamaica Plain, MA 02130, United States.
authors.forename: Susan E
authors.initials: SE
authors.lastname: Sloan
authors.forename: Cathleen
authors.initials: C
authors.lastname: Hanlon
authors.forename: William
authors.initials: W
authors.lastname: Weldon
authors.forename: Michael
authors.initials: M
authors.lastname: Niezgoda
authors.forename: Jesse
authors.initials: J
authors.lastname: Blanton
authors.forename: Josh
authors.initials: J
authors.lastname: Self
authors.forename: Kirk J
authors.initials: KJ
authors.lastname: Rowley
authors.forename: Robert B
authors.initials: RB
authors.lastname: Mandell
authors.forename: Gregory J
authors.initials: GJ
authors.lastname: Babcock
authors.forename: William D
authors.initials: WD
authors.lastname: Thomas
authors.forename: Charles E
authors.initials: CE
authors.lastname: Rupprecht
authors.forename: Donna M
authors.initials: DM
authors.lastname: Ambrosino
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D002460:Cell Line; D006224:Cricetinae; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D016056:Immunodominant Epitopes / Q000235:genetics / Q000276:immunology; D012889:Mephitidae; D008647:Mesocricetus; D051379:Mice; D008822:Mice, Transgenic; D011818:Rabies / Q000276:immunology / Q000517:prevention & control; D011820:Rabies virus / Q000235:genetics / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D016056:Immunodominant Epitopes; D014764:Viral Proteins