title: CD83 is a regulator of murine B cell function in vivo.
abstract: The transmembrane glycoprotein CD83 has been described as a specific maturation marker for dendritic cells and several lines of evidence suggest that CD83 regulates thymic T cell maturation as well as peripheral T cell activation. Here we show for the first time that CD83 is involved also in the regulation of B cell function. CD83 is up-regulated on activated B cells in vivo, specifically in the draining lymph nodes of Leishmania major-infected mice. The ubiquitous transgenic (Tg) expression of CD83 interferes with Leishmania-specific T cell-dependent and with T cell-independent antibody production. This defect is restricted to the B cell population since the antigen-specific T cell response of CD83Tg mice to L. major infection is unchanged. The defective immunoglobulin (Ig) response is due to Tg expression of CD83 on the B cells because wild-type B cells display normal antigen-specific responses in CD83Tg hosts and CD83Tg B cells do not respond to immunization in a mixed wild-type/CD83Tg bone marrow chimera. Finally, the treatment of non-Tg C57BL/6 mice with anti-CD83 mAb induces a dramatic increase in the antigen-specific IgG response to immunization, thus demonstrating a regulatory role for naturally induced CD83 on wild-type B cells.
authors.affiliation: Bernhard-Nocht-Institute for Tropical Medicine, Hamburg, Germany. MBreloer@aol.com
authors.forename: Minka
authors.initials: M
authors.lastname: Breloer
authors.forename: Birte
authors.initials: B
authors.lastname: Kretschmer
authors.forename: Katja
authors.initials: K
authors.lastname: Lüthje
authors.forename: Svenja
authors.initials: S
authors.lastname: Ehrlich
authors.forename: Uwe
authors.initials: U
authors.lastname: Ritter
authors.forename: Thomas
authors.initials: T
authors.lastname: Bickert
authors.forename: Christiane
authors.initials: C
authors.lastname: Steeg
authors.forename: Simon
authors.initials: S
authors.lastname: Fillatreau
authors.forename: Kai
authors.initials: K
authors.lastname: Hoehlig
authors.forename: Vassiliki
authors.initials: V
authors.lastname: Lampropoulou
authors.forename: Bernhard
authors.initials: B
authors.lastname: Fleischer
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis; D015703:Antigens, CD / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D005260:Female; D007136:Immunoglobulins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D018320:Leishmania major / Q000276:immunology; D016773:Leishmaniasis, Cutaneous / Q000276:immunology; D008213:Lymphocyte Activation / Q000235:genetics; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D015854:Up-Regulation / Q000276:immunology; D000097802:CD83 Antigen
chemical_list: D000913:Antibodies, Protozoan; D015703:Antigens, CD; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen