Expression of suppressor of cytokine signaling 3 (SOCS3) and cytokine-inducible Src homology 2-containing protein (CIS) induced in Burkholderia pseudomallei--infected mouse macrophages requires bacterial internalization.
We recently reported that Burkholderia pseudomallei was able to activate the expression of suppressor of cytokine signaling 3 (SOCS3) and cytokine-inducible Src homology 2-containing protein (CIS). In the present study, we presented evidence showing that the induction of these negative regulators was most probably triggered from within rather than at the cell surface of mouse macrophage cell line (RAW264.7) suggesting that macrophage activation most likely requires the interaction of bacteria with a putative host cell cytoplasmic component(s).
Ekchariyawat
P
P
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
Pudla
S
S
Limposuwan
K
K
Arjcharoen
S
S
Sirisinha
S
S
Utaisincharoen
P
P
D000818:Animals; D016957:Burkholderia pseudomallei / Q000502:physiology*; D002460:Cell Line; D008264:Macrophages / Q000378:metabolism / Q000382:microbiology; D008554:Melioidosis / Q000378:metabolism* / Q000382:microbiology; D051379:Mice; D015398:Signal Transduction; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000378:metabolism*
D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins; C491411:Socs3 protein, mouse