Influence of coagulation factor zymogens on the infectivity of adenoviruses pseudotyped with fibers from subgroup D.
Recent evidence supports a role for vitamin K-dependent coagulation zymogens in adenovirus serotype 5 (Ad5, subgroup C) infection of hepatocytes. Here, we assessed the effect of virus-zymogen interaction on cellular transduction using a panel of fiber (f)-pseudotyped viruses derived from subgroup D (f47, f33, f24, f45, f17, f30). Each virus directly bound factor X (FX) as determined by surface plasmon resonance, resulting in enhanced cell surface binding. Infection of HepG2 cells was promoted by FX but not by FVII or FIX, while transduction of CHO cells was blocked in heparan sulfate proteoglycan-deficient cells. This suggests a broad role for FX in adenovirus infectivity.
Parker
Alan L
AL
British Heart Foundation Glasgow Cardiovascular Research Centre, Division of Cardiovascular and Medical Sciences, University of Glasgow, 126 University Place, Glasgow G12 8TA, United Kingdom.
McVey
John H
JH
Doctor
Jessica H
JH
Lopez-Franco
Oscar
O
Waddington
Simon N
SN
Havenga
Menzo J E
MJ
Nicklin
Stuart A
SA
Baker
Andrew H
AH
D000256:Adenoviridae / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001779:Blood Coagulation Factors / Q000378:metabolism*; D006224:Cricetinae; D004792:Enzyme Precursors / Q000378:metabolism*; D006801:Humans; D010802:Phylogeny; D011485:Protein Binding; D053586:Virus Internalization
D001779:Blood Coagulation Factors; D004792:Enzyme Precursors