title: Ultra-performance liquid chromatography-tandem mass spectrometry for the determination of atypical antipsychotics and some metabolites in in vitro samples.
abstract: The ultra-performance liquid chromatography-electrospray tandem mass spectrometry (UPLC-ESI-MS/MS) method has been developed to perform the determination of quetiapine, perospirone, aripiprazole and quetiapine sulfoxide in in vitro samples in less than 3 min. The UPLC separation was carried out using an Acquity UPLC BEH C18 column (100 mm x 2.1mm i.d., 1.7 microm particle size) that provided high efficiency and resolution in combination with high linear velocities. The UPLC system was coupled to a Waters Micromass Quattro Premier XE tandem quadrupole mass spectrometer. This system permits high-speed data acquisition without peak intensity degradation, and produces sharp and narrow chromatographic peaks (w(h) about 2.5s) of compounds. The determination was performed in multiple reaction monitoring (MRM) mode. The quantification parameters of the developed method were established, obtaining instrumental LODs lower than 0.005 microg/l and a repeatability at a low concentration level lower than 10% CV (n=10). Finally, the method was successfully applied to the analysis of atypical antipsychotics and some metabolites in in vitro samples.
authors.affiliation: Clinical Pharmaceutical Research Institute, XiangYa Second Hospital, Central South University, Changsha 410011, China.
authors.forename: Kun-Yan
authors.initials: KY
authors.lastname: Li
authors.forename: Yan-Gang
authors.initials: YG
authors.lastname: Zhou
authors.forename: Hua-Yi
authors.initials: HY
authors.lastname: Ren
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Bi-Kui
authors.initials: BK
authors.lastname: Zhang
authors.forename: Huan-De
authors.initials: HD
authors.lastname: Li
mesh_terms: D014150:Antipsychotic Agents / Q000032:analysis*; D000068180:Aripiprazole; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003987:Dibenzothiazepines / Q000032:analysis*; D007211:Indoles / Q000032:analysis*; D054833:Isoindoles; D010879:Piperazines / Q000032:analysis*; D000069348:Quetiapine Fumarate; D015363:Quinolones / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013844:Thiazoles / Q000032:analysis*
chemical_list: D014150:Antipsychotic Agents; D003987:Dibenzothiazepines; D007211:Indoles; D054833:Isoindoles; D010879:Piperazines; D015363:Quinolones; D013844:Thiazoles; D000069348:Quetiapine Fumarate; D000068180:Aripiprazole; C065533:perospirone