Analysis of micafungin in serum by capillary zone electrophoresis.
A method is developed for measuring the concentration of micafungin, an echinocandin antifungal agent, in serum, using capillary zone electrophoresis. With a 0.1M borate buffer (pH 10.0) as the run buffer, detection is carried out at 200 nm. Pretreatment is performed by adding acetonitrile to serum (1:2) for deproteinization, allowing the supernatant fluid to be taken for measurement. The detection limit of the assay is 0.5 mg/L at a signal-to-noise ratio of 3.0. Coefficients of variation for intra- and inter-assay precision are 3.45-4.47% and 2.61-7.15%, respectively, at a nominal concentration of 5.0-25.0 mg/L. Their recovery rates are 92-110%. It is convenient for the monitoring of micafungin therapy in patients contracting clinically important deep mycoses.
Kitahashi
Toshihiro
T
Department of Laboratory Medicine, Kinki University School of Medicine, 377-2 Ohno-higashi, Osakasayama, Osaka 589-8511, Japan. kitahasi@med.kindai.ac.jp
Furuta
Itaru
I
D000935:Antifungal Agents / Q000097:blood*; D054714:Echinocandins; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D055666:Lipopeptides; D008074:Lipoproteins / Q000097:blood*; D000077551:Micafungin; D010456:Peptides, Cyclic / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000935:Antifungal Agents; D054714:Echinocandins; D055666:Lipopeptides; D008074:Lipoproteins; D010456:Peptides, Cyclic; D000077551:Micafungin