title: Effect of Bauhinia racemosa stem bark on N-nitrosodiethylamine-induced hepatocarcinogenesis in rats.
abstract: The aim of this study is to investigate the antioxidant defense system induced by the methanol extract of Bauhinia racemosa L.(MEBR) against N-nitrosodiethylamine (NDEA)-induced hepatocarcinogenesis in Wister albino rats. The effects of MEBR on surface visible macroscopic (Morphometry) liver lesions (neoplastic nodules) and the levels of serum enzymes, lipid peroxidation and antioxidants were evaluated in NDEA-induced hepatocarcinogenesis in rats. In rats treated, with NDEA, significantly elevated levels of serum enzymes (SGOT, SGPT and ALP), bilirubin and decreased levels of protein and uric acid were observed. Significantly elevated amount of malondialdehyde (MDA), the end product of lipidperoxidation, indicated higher levels of lipid peroxidation, which was accompanied by significantly decreased levels of antioxidants like vitamin C, vitamin E, reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT). Administration of MEBR was able to suppress nodule development/hepatocellular lesion formation in rats. The extract treatment increases in antioxidant levels and dramatic decreases in lipid peroxidation levels. MEBR also produced a protective effect by decreasing the level of serum enzymes, bilirubin and increased the protein and uric acid levels. The results suggest that MEBR exert chemopreventive effects by suppressing nodule development and decreasing lipid peroxidation and enhancing the levels of antioxidants in NDEA carcinogenesis by reducing the formation of free radicals.
authors.affiliation: Natural Products Research Laboratories, JKK Nataraja College of Pharmacy, Komarapalayam, Namakkal-638 183, Tamilnadu, India. sambathju2002@yahoo.co.in
authors.forename: Ramanathan Sambath
authors.initials: RS
authors.lastname: Kumar
authors.forename: Rajagopal Shanmuga
authors.initials: RS
authors.lastname: Sunderam
authors.forename: Thangavel
authors.initials: T
authors.lastname: Sivakumar
authors.forename: Palanivel
authors.initials: P
authors.lastname: Sivakumar
authors.forename: Raman
authors.initials: R
authors.lastname: Sureshkumar
authors.forename: Rengaswamy
authors.initials: R
authors.lastname: Kanagasabi
authors.forename: Manickum
authors.initials: M
authors.lastname: Vijaya
authors.forename: Baskaran Perumal
authors.initials: BP
authors.lastname: Perumal
authors.forename: Malaya
authors.initials: M
authors.lastname: Gupta
authors.forename: Upal Kanti
authors.initials: UK
authors.lastname: Mazumdar
authors.forename: Mani Senthil
authors.initials: MS
authors.lastname: Kumar
authors.forename: Kuppuswamy Asok
authors.initials: KA
authors.lastname: Kumar
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000477:Alkylating Agents; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001219:Aspartate Aminotransferases / Q000097:blood; D036481:Bauhinia; D001663:Bilirubin / Q000097:blood; D006528:Carcinoma, Hepatocellular / Q000139:chemically induced / Q000517:prevention & control*; D018890:Chemoprevention; D004052:Diethylnitrosamine; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008113:Liver Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008517:Phytotherapy / Q000379:methods*; D024301:Plant Bark; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000477:Alkylating Agents; D000975:Antioxidants; D010936:Plant Extracts; D004052:Diethylnitrosamine; D008315:Malondialdehyde; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D001663:Bilirubin