[Effects of butyrylchitosan on expression of proliferating cell nuclear antigen in fibroblast proliferation of rabbit eyes after filtering operation].
OBJECTIVE
To investigate the effect of butyrylchitosan on the expression of proliferating cell nuclear antigen ( PCNA) in fibroblast proliferation of rabbit eyes after filtering operation.


METHODS
Twenty-four New Zealand rabbits were randomly divided into 2 groups, with 12 rabbits in each group. Rabbits in one group received butyrylchitosan under scleral patch of trabeculectomy in right eyes and trabeculectomy in left eyes (trabeculectomy group). Rabbits in the other group received mitomycin C (MMC) in trabeculectomy in right eyes (MMC group) and without operation in left eyes. Rabbits were killed 1, 4, and 12 weeks after operations. Immunohistochemical staining was used to detected PCNA expression in fibroblast.


RESULTS
After use of butyrylchitosan, the PCNA expression significantly decreased compared with trabeculectomy group (P < 0. 001). PCNA expression in MMC group was significantly lower than in trabeculectomy group (P <0. 001).


CONCLUSIONS
Using butyrylchitosan under scleral patch of trabeculectomy decreases PCNA expression in proliferating cell and inhibits the scarring at filtering site.
Wei
Ping
P
Department of Ophthalmology, the First People's Hospital of Lanzhou City, Lanzhou 730050, China. weiping966@163.com
Wang
Ai-qin
AQ
Lei
Xun-wen
XW
Cao
Zhi-xin
ZX
Li
Xiao-lin
XL
Wang
Qin
Q
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000031:analogs & derivatives / Q000494:pharmacology; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D018463:Filtering Surgery; D008297:Male; D008567:Membranes, Artificial; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis*; D011817:Rabbits
D008567:Membranes, Artificial; D018809:Proliferating Cell Nuclear Antigen; D048271:Chitosan