title: Large scale preparation of the mammalian high mobility group protein A2 for biophysical studies.
abstract: Due to asymmetrical charge distribution of the mammalian high mobility group protein A2 (HMGA2), which makes HMGA2 bind to both cation- and anion-exchange columns, we developed a rapid procedure for purifying HMGA2 in the milligram range. This purification procedure greatly facilitated biophysical studies, which require large amounts of the protein.
authors.affiliation: Department of Chemistry and Biochemistry, Florida International University, Miami, FL 33199, USA.
authors.forename: Tengjiao
authors.initials: T
authors.lastname: Cui
authors.forename: Suzanne
authors.initials: S
authors.lastname: Joynt
authors.forename: Victor
authors.initials: V
authors.lastname: Morillo
authors.forename: Maria
authors.initials: M
authors.lastname: Baez
authors.forename: Zhichun
authors.initials: Z
authors.lastname: Hua
authors.forename: Xiaotang
authors.initials: X
authors.lastname: Wang
authors.forename: Fenfei
authors.initials: F
authors.lastname: Leng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002852:Chromatography, Ion Exchange; D015870:Gene Expression; D025743:HMGA2 Protein / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D025743:HMGA2 Protein; D011994:Recombinant Proteins