title: A label-free photonic crystal biosensor imaging method for detection of cancer cell cytotoxicity and proliferation.
abstract: A label-free method for detecting the attachment of human cancer cells to a biosensor surface for rapid screening for biological activity is described, in which attachment of a cell results in highly localized increase of the resonant reflected wavelength of a photonic crystal narrowband reflectance filter incorporated into a standard 96-well microplate. An imaging detection instrument is used to determine the spatial distribution of attached cells by mapping the shift in reflected resonant wavelength as a function of position. The method enables monitoring of cancer cell attachment, cell proliferation, and cell detachment that is induced by exposure of the cells to drug compounds. We demonstrate the efficacy of this method as an early screening technique for the rapid quantification of the rate of cancer cell proliferation on the sensor surface, and subsequently as a means for quantifying cell detachment resulting from apoptosis that is induced by exposure of the cells to cytotoxic chemicals.
authors.affiliation: Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
authors.forename: Leo L
authors.initials: LL
authors.lastname: Chan
authors.forename: Saujanya L
authors.initials: SL
authors.lastname: Gosangari
authors.forename: Kenneth L
authors.initials: KL
authors.lastname: Watkin
authors.forename: Brian T
authors.initials: BT
authors.lastname: Cunningham
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002448:Cell Adhesion / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000494:pharmacology; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D020128:Inhibitory Concentration 50
chemical_list: D004317:Doxorubicin