Dense molecular marking reveals genetic diversity in morphologically similar isolates of the nematophagous fungus Duddingtonia flagrans.
Three morphologically similar isolates of Duddingtonia flagrans [(Duddington) R. C. Cooke] viz., Df-2550, Df-2507 and Df-BJ were subjected to RAPD (Randomly Amplified Polymorphic DNA) and SRFA (Selective Fragment Length Amplification) mode of DNA fingerprinting analysis to generate 233 different anonymous DNA markers. Mean number of alleles per primer/primer pair for RAPD and SRFA primers was 13.75 and 29.66 respectively. Phylogenetic analysis through bootstrapping of 1000 simulated replicates of the data set demonstrated that Df-2550 was ancestral in the group of three and did not align with Df-2507 and Df-BJ, which appeared to diversify recently and therefore remained at the end of the phylogenetic tree. Genomic islands were also identified by three SRFA primer pairs, where Df-2550 aligned with Df-BJ, which is reverse to the master consensus-grouping pattern. Scanning image of the amplicon profiles when represented graphically generated unique molecular signature for the isolates.
Mukhopadhyaya
P N
PN
Biotechnology Laboratory, National Dairy Development Board, Anand-388001, Gujarat, India. pratapm@nddb.coop
Nagee
A
A
Sanyal
P K
PK
D000818:Animals; D001203:Ascomycota / Q000166:cytology* / Q000235:genetics* / Q000302:isolation & purification; D016172:DNA Fingerprinting; D005819:Genetic Markers; D014644:Genetic Variation; D003904:Mitosporic Fungi / Q000166:cytology* / Q000235:genetics* / Q000302:isolation & purification; D009348:Nematoda / Q000382:microbiology*
D005819:Genetic Markers