[Synthesis of gastrodin by microbial transformation].
OBJECTIVE
To synthesize gastrodin via biotransformation of microorganism using p-hydroxybenzaldehyde as substrate.


METHODS
Using resting cell techniques with TLC and HPLC analysis, a strain of Rhizopus chinensis Staito AS3. 1165 which has the capability of biotransforming p-hydroxybenzaldehyde into gastrodin was screened from 50 strains of microorganisms. Preparative scale biotransformation was carried out under the optimal transforming condition. The product was obtained by chromatography, and characterized on the basis of its physical and spectral data.


RESULTS
The product was identified as gastrodin on the basis of its 1H NMR, 13C NMR and EI-MS spectral data.


CONCLUSIONS
The fact that gastrodin could be synthesized by microbial transformation implies a new approach to gastrodin production. As a result, this research will be of a great economic and social value.
Zhu
Hong-li
HL
College of Life Science, Northwest University, Xi' an 710069, China. zhuyjw@nwu.edu.cn
Song
Ji-rong
JR
Huang
Jian-xin
JX
Zhang
Jia
J
Ma
Zhen-Yu
ZY
Yang
Ming-yan
MY
D001547:Benzaldehydes / Q000737:chemistry / Q000378:metabolism*; D001592:Benzyl Alcohols / Q000737:chemistry; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005960:Glucosides / Q000096:biosynthesis* / Q000737:chemistry; D008954:Models, Biological; D015394:Molecular Structure; D012233:Rhizopus / Q000378:metabolism*
D001547:Benzaldehydes; D001592:Benzyl Alcohols; D005960:Glucosides; C045345:gastrodin; C011483:4-hydroxybenzaldehyde