Molecular cloning of porcine RP105/MD-1 involved in recognition of extracellular phosphopolysaccharides from Lactococcus lactis ssp. cremoris.
In this study, we cloned the cDNAs encoding porcine RP105 (poRP105) and porcine MD-1 (poMD-1) from Peyer's patches of adult swine. The complete open reading frames of poRP105 and poMD-1 contain 1986 and 480bp and encode 661 and 159 amino acid residues, respectively. These two proteins were more similar to the human (77.6% and 76.5% amino acid identity) than the mouse counterparts (70.0% and 71.1% amino acid identity). The results of several experiments in cells cotransfected with poRP105 and poMD-1 indicated both lipopolysaccharide and extracellular phosphopolysaccharide from Lactococcus lactis subsp. cremoris (Lc.cremoris) strongly activate nuclear factor-kappaB and induce the expression of various cytokines via RP105. These effects were mediated by phosphatidylinositol 3-kinase and Bruton's tyrosine kinase. Thus, we identified extracellular polysaccharide from Lc.cremoris as an active substance that can induce immune activation via RP105 and MD-1.
Tohno
Masanori
M
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Aobaku, Sendai 981-8555, Japan.
Shimazu
Tomoyuki
T
Ueda
Wataru
W
Anzawa
Daisuke
D
Aso
Hisashi
H
Nishimura
Junko
J
Kawai
Yasushi
Y
Saito
Yasuo
Y
Saito
Tadao
T
Kitazawa
Haruki
H
D000077329:Agammaglobulinaemia Tyrosine Kinase; D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology*; D000954:Antigens, Surface / Q000235:genetics / Q000276:immunology*; D003001:Cloning, Molecular; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D013294:Lactococcus lactis / Q000276:immunology*; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000378:metabolism; D000081082:Phosphoinositide-3 Kinase Inhibitors; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D013552:Swine / Q000235:genetics / Q000276:immunology*
D015703:Antigens, CD; D000954:Antigens, Surface; D016207:Cytokines; D016328:NF-kappa B; D000081082:Phosphoinositide-3 Kinase Inhibitors; D011135:Polysaccharides, Bacterial; D011505:Protein-Tyrosine Kinases; D000077329:Agammaglobulinaemia Tyrosine Kinase; C000625951:Btk protein, mouse