Enantioselective separation of phenylglycidates by capillary electrophoresis employing sulfated beta-cyclodextrin as chiral selector.
A capillary electrophoresis (CE) method for the enantioseparation of phenylglycidates has been developed. Successful enantioseparation was achieved using sulfated beta-cyclodextrin as chiral selector in a phosphate buffer. The effects of varying pH, sulfated beta-cyclodextrin concentration and electrophoresis voltage were systematically investigated and the optimized separation conditions were thus obtained. When the migration time was set at the threshold value, it was found that the best enantioseparation was obtained at 10 kV with 3% (w/v) sulfated beta-cyclodextrin at pH 6.5. A range of substituted phenylglycidates were successfully separated using the method and the results shown to be superior to those obtained using gas chromatography (GC).
Wang
Jianjun
J
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China.
Yuan
Qipeng
Q
Evans
David G
DG
Yang
Liu
L
Zheng
Guojun
G
Sun
Wanru
W
D019075:Electrophoresis, Capillary / Q000379:methods*; D005998:Glycine / Q000031:analogs & derivatives / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D047392:beta-Cyclodextrins / Q000737:chemistry*
D047392:beta-Cyclodextrins; C031215:betadex; D005998:Glycine