Antioxidant properties of neohesperidin dihydrochalcone: inhibition of hypochlorous acid-induced DNA strand breakage, protein degradation, and cell death.
Neohesperidin dihydrochalcone (NHDC), a non-nutritive sweetening agent, is simply produced by hydrogenation of neohesperidin. The aim of this study is to evaluate the antioxidant and radical scavenging properties of neohesperidin dihydrochalcone and other structurally related compounds (phloridzin, neohesperidin) toward different reactive radical and oxygen species including .ABTS+, .O2-, .OH, H2O2, and HOCl in vitro. NHDC showed remarkable radical scavenging activity against stable radical and reactive oxygen species (ROS) in concentration dependent manner. Especially, NHDC was the most potent inhibitor of H2O2 and HOCl. NHDC showed HOCl scavenging activity of 93.5% and H2O2 scavenging property of 73.5% which was more than those of all the tested compounds including ascorbic acid and BHT. Moreover, NHDC could inhibit protein degradation, plasmid DNA strand cleavage and HIT-T15, HUVEC cell death from HOCl attack while mannitol, BHT, and ascorbic acid could not protect them effectively. These results suggest that NHDC is a potent antioxidant, especially it is evaluated as a novel HOCl scavenger. This study implies the possibility of therapeutic effect of NHDC on ROS-related inflammatory diseases.
Choi
Je-Min
JM
Department of Biotechnology, College of Engineering, Yonsei University, Seoul, Republic of Korea.
Yoon
Byoung-Seok
BS
Lee
Sang-Kyou
SK
Hwang
Jae-Kwan
JK
Ryang
Ryung
R
D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D047188:Chalcones / Q000494:pharmacology*; D006224:Cricetinae; D053837:DNA Cleavage / Q000187:drug effects; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006569:Hesperidin / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D006997:Hypochlorous Acid / Q000378:metabolism / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D012709:Serum Albumin / Q000378:metabolism; D013549:Sweetening Agents / Q000494:pharmacology*
D047188:Chalcones; D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D012709:Serum Albumin; D013549:Sweetening Agents; C013613:neohesperidin dihydrochalcone; D006997:Hypochlorous Acid; D006861:Hydrogen Peroxide; D006569:Hesperidin