title: ELISA IgG concentrations and opsonophagocytic activity following pneumococcal protein D conjugate vaccination and relationship to efficacy against acute otitis media.
abstract: We explored the relationship between efficacy against acute otitis media (AOM) and both ELISA anti-polysaccharide IgG geometric mean concentrations (GMCs) and opsonophagocytic (OPA) geometric mean titres (GMTs) following primary and booster vaccination with pneumococcal protein D (Haemophilus influenzae-derived) conjugate vaccine. It was possible to distinguish between the OPA GMTs of low and high efficacy serotypes, however no such distinction was evident for ELISA GMCs. Also, there was a trend towards lower ELISA and OPA serotype-specific responses in subjects who developed AOM compared to controls. We conclude that serotype-specific OPA GMTs are an important endpoint to consider for evaluation of pneumococcal conjugate vaccines with respect to the protective efficacy against AOM (Study ID: 347414/NCT00119743).
authors.affiliation: GlaxoSmithKline Biologicals, Rixensart, Belgium.
authors.forename: Lode
authors.initials: L
authors.lastname: Schuerman
authors.forename: Roman
authors.initials: R
authors.lastname: Prymula
authors.forename: Isabelle
authors.initials: I
authors.lastname: Henckaerts
authors.forename: Jan
authors.initials: J
authors.lastname: Poolman
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood*; D001426:Bacterial Proteins / Q000276:immunology; D015415:Biomarkers; D002352:Carrier Proteins / Q000276:immunology; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007117:Immunization, Secondary; D007072:Immunoglobulin D / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood; D007223:Infant; D008074:Lipoproteins / Q000276:immunology; D010033:Otitis Media / Q000517:prevention & control*; D010587:Phagocytosis; D011008:Pneumococcal Infections / Q000517:prevention & control*; D022242:Pneumococcal Vaccines / Q000276:immunology*; D018074:Vaccines, Conjugate / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D015415:Biomarkers; D002352:Carrier Proteins; D007072:Immunoglobulin D; D007074:Immunoglobulin G; D008074:Lipoproteins; D022242:Pneumococcal Vaccines; D018074:Vaccines, Conjugate; C067989:glpQ protein, Haemophilus influenzae