Identification of immunoreactive antigens in membrane proteins enriched fraction from Francisella tularensis LVS.
Francisella tularensis is a Gram-negative, facultative intracellular bacterium causing disease in many mammalian species. The low infectious dose of F. tularensis and the ease of air-borne transmission are the main features responsible for the classification of this bacterium as a potential biological weapon. The live attenuated strain of F. tularensis live vaccine strain (LVS) is currently only effective vaccine against tularemia, however, this type of vaccine has not been approved for human use. In the presented study, sub-immunoproteome analysis was performed to search for new immunogenic proteins of Francisella tularensis LVS grown under different conditions. By this approach 35 immunoreactive antigens were identified, 19 of them showed to be novel immunogens. In conclusion, sub-immunoproteome analysis resulted in successful identification of novel immunoreactive proteins.
Janovská
Sylva
S
Institute of Molecular Pathology, University of Defence, Trebesska 1575, Hradec Kralove, Czech Republic. janovska@pmfhk.cz
Pávková
Ivona
I
Hubálek
Martin
M
Lenco
Juraj
J
Macela
Ales
A
Stulík
Jirí
J
D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D019295:Computational Biology; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005604:Francisella tularensis / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007525:Isoelectric Focusing; D008565:Membrane Proteins / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
D000942:Antigens, Bacterial; D007106:Immune Sera; D008565:Membrane Proteins