In vivo and in vitro anaerobic mating in Candida albicans.
Candida albicans cells of opposite mating types are thought to conjugate during infection in mammalian hosts, but paradoxically, the mating-competent opaque state is not stable at mammalian body temperatures. We found that anaerobic conditions stabilize the opaque state at 37 degrees C, block production of farnesol, and permit in vitro mating at 37 degrees C at efficiencies of up to 84%. Aerobically, farnesol prevents mating because it kills the opaque cells necessary for mating, and as a corollary, farnesol production is turned off in opaque cells. These in vitro observations suggest that naturally anaerobic sites, such as the efficiently colonized gastrointestinal (GI) tract, could serve as niches for C. albicans mating. In a direct test of mating in the mouse GI tract, prototrophic cells were obtained from auxotrophic parent cells, confirming that mating will occur in this organ. These cells were true mating products because they were tetraploid, mononuclear, and prototrophic, and they contained the heterologous hisG marker from one of the parental strains.
Dumitru
Raluca
R
School of Biological Sciences, University of Nebraska, Lincoln, NE 68588-0666, USA.
Navarathna
Dhammika H M L P
DH
Semighini
Camile P
CP
Elowsky
Christian G
CG
Dumitru
Razvan V
RV
Dignard
Daniel
D
Whiteway
Malcolm
M
Atkin
Audrey L
AL
Nickerson
Kenneth W
KW
D000693:Anaerobiosis / Q000502:physiology; D000818:Animals; D002176:Candida albicans / Q000166:cytology* / Q000235:genetics* / Q000378:metabolism; D003227:Conjugation, Genetic / Q000502:physiology; D005204:Farnesol / Q000378:metabolism / Q000494:pharmacology; D005260:Female; D041981:Gastrointestinal Tract / Q000382:microbiology* / Q000502:physiology; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects / Q000235:genetics; D049770:Genes, Mating Type, Fungal / Q000187:drug effects / Q000235:genetics*; D005812:Genes, Switch / Q000235:genetics*; D051379:Mice; D008815:Mice, Inbred Strains; D008856:Microscopy, Fluorescence; D008858:Microscopy, Phase-Contrast; D010641:Phenotype; D015398:Signal Transduction; D013045:Species Specificity; D013696:Temperature
D005204:Farnesol