title: Promoting plasticity in the spinal cord with chondroitinase improves functional recovery after peripheral nerve repair.
abstract: Functional recovery after peripheral nerve repair in humans is often disappointing. A major reason for this is the inaccuracy of re-innervation of muscles and sensory structures. We hypothesized that promoting plasticity in the spinal cord, through digestion of chondroitin sulphate proteoglycans (CSPGs) with chondroitinase ABC (ChABC), might allow the CNS to compensate for inaccurate peripheral re-innervation and improve functional recovery. The median and ulnar nerves were injured and repaired to produce three grades of inaccuracy of peripheral re-innervation by (i) crush of both nerves; (ii) correct repair of median to median and ulnar to ulnar; and (iii) crossover of the median and ulnar nerves. Mapping of the motor neuron pool of the flexor carpi radialis muscle showed precise re-innervation after nerve crush, inaccurate regeneration after correct repair, more inaccurate after crossover repair. Recovery of forelimb function, assessed by skilled paw reaching, grip strength and sensory testing varied with accuracy of re-innervation. This was not due to differences in the number of regenerated axons. Single injections of ChABC into the spinal cord led to long-term changes in the extracellular matrix, with hyaluronan and neurocan being removed and not fully replaced after 8 weeks. ChABC treatment produce increased sprouting visualized by MAP1BP staining and improved functional recovery in skilled paw reaching after correct repair and in grip strength after crossover repair. There was no hyperalgesia. Enhanced plasticity in the spinal cord, therefore, allows the CNS to compensate for inaccurate motor and sensory re-innervation of the periphery, and may be a useful adjunct therapy to peripheral nerve repair.
authors.affiliation: Centre for Brain Repair, Department of Clinical Neurosciences, University of Cambridge, Cambridge, UK.
authors.forename: Clare M
authors.initials: CM
authors.lastname: Galtrey
authors.forename: Richard A
authors.initials: RA
authors.lastname: Asher
authors.forename: Fatiha
authors.initials: F
authors.lastname: Nothias
authors.forename: James W
authors.initials: JW
authors.lastname: Fawcett
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology; D002452:Cell Count; D019765:Chondroitin ABC Lyase / Q000008:administration & dosage*; D004195:Disease Models, Animal; D005109:Extracellular Matrix / Q000187:drug effects; D005552:Forelimb / Q000503:physiopathology; D008297:Male; D008475:Median Nerve / Q000293:injuries / Q000503:physiopathology / Q000601:surgery; D009068:Movement / Q000502:physiology; D018482:Muscle, Skeletal / Q000503:physiopathology; D009416:Nerve Regeneration / Q000187:drug effects / Q000502:physiology; D009473:Neuronal Plasticity / Q000187:drug effects* / Q000502:physiology; D009929:Organ Size; D010146:Pain / Q000503:physiopathology; D059348:Peripheral Nerve Injuries; D010525:Peripheral Nerves / Q000503:physiopathology / Q000601:surgery; D051381:Rats; D020127:Recovery of Function / Q000187:drug effects* / Q000502:physiology; D013116:Spinal Cord / Q000187:drug effects* / Q000503:physiopathology; D014459:Ulnar Nerve / Q000293:injuries / Q000503:physiopathology / Q000601:surgery
chemical_list: D019765:Chondroitin ABC Lyase