Suppression of azoxymethane-induced colonic premalignant lesion formation by coenzyme Q10 in rats.
Reactive oxygen species cause damage to proteins, lipids and DNA. Coenzyme Q10 (CoQ10) is a compound with mitochondrial bioenergetic functions. The reduced form of CoQ10 shows antioxidant activity. In the present study, effects of CoQ10 on development of azoxymethane (AOM)-induced aberrant crypt foci (ACF) and mucin-depleted foci (MDF) in F344 male rats were investigated. To induce ACF and MDF, 6-week old rats were given two weekly subcutaneous injections of AOM (15 mg/kg body weight) and also received a control diet or experimental diets containing CoQ10 (200 or 500 ppm) for 4 weeks, starting one day before the first dose of AOM. At 10 weeks of age, all animals were sacrificed and their colons were evaluated for numbers and sizes of ACF and MDF. Administration of 200 and 500 ppm CoQ10 resulted in reduction of ACF numbers, to 77% and 68% of the carcinogen control value, respectively. The percentages of ACF consisting of more than 4 crypts in these groups were also significantly lower than in the controls. Treatment with 500 ppm CoQ10 furthermore decreased the number of sialomucin-producing ACF and MDF per colon to 42% and 38% of the carcinogen control value without CoQ10, respectively. These results suggest that CoQ10 may be an effective chemopreventive agent against colon carcinogenesis.
Sakano
Katsuhisa
K
Cancer Prevention Basic Research Project, National Cancer Center Research Institute, Tokyo 104-0045, Japan.
Takahashi
Mami
M
Kitano
Mitsuaki
M
Sugimura
Takashi
T
Wakabayashi
Keiji
K
D000818:Animals; D001397:Azoxymethane; D003067:Coenzymes; D003106:Colon / Q000187:drug effects* / Q000473:pathology*; D008297:Male; D011230:Precancerous Conditions / Q000473:pathology / Q000517:prevention & control*; D051381:Rats; D011916:Rats, Inbred F344; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000494:pharmacology; D014815:Vitamins / Q000494:pharmacology*
D003067:Coenzymes; D014815:Vitamins; D014451:Ubiquinone; C024989:coenzyme Q10; D001397:Azoxymethane