Allelic distributions of CYP2D6 gene copy number variation in the Eastern Han Chinese population.
OBJECTIVE
The human cytochrome P450 2D6 (CYP2D6) gene copy number variation, involving CYP2D6 gene deletion (CYP2D6*5) and duplication or multiduplication (CYP2D6*xN), can result in reduced or increased metabolism of many clinically used drugs. The identification of CYP2D6*5 and CYP2D6*xN and the investigation of their allelic distributions in ethnic populations can be important in determining the right drug and dosage for each patient.


METHODS
The CYP2D6*5 and CYP2D6 genes, and CYP2D6 gene duplication were identified by 2 modified long PCR, respectively. To determine duplicated alleles, a novel long PCR was developed to amplify the entire duplicated CYP2D6 gene which was used as template for subsequent PCR amplification. A total of 363 unrelated Eastern Han Chinese individuals were analyzed for CYP2D6 gene copy number variation.


RESULTS
The frequency of CYP2D6*5 and CYP2D6*xN were 4.82% (n=35) and 0.69% (n=5) in the Eastern Han Chinese population, respectively. Of the 5 duplicated alleles, 3 were CYP2D6*1xN and 2 were CYP2D6*10xN. One individual was a carrier of both CYP2D6*5 and CYP2D6*1xN. Taken together, the CYP2D6 gene rearrangements were present in 10.74% of subjects.


CONCLUSIONS
Allelic distributions of the CYP2D6 gene copy number variation differ among Chinese from different regions, indicating ethnic variety in Chinese. Long PCR are convenient, cost effective, specific and semiquantitative for the detection of the CYP2D6 gene copy number variation, and amplification of the entire duplicated CYP2D6 gene is necessary for the accurate identification of duplicated alleles.
Sheng
Hai-hui
HH
National Engineering Center for Biochip at Shanghai, Shanghai 201203, China.
Zeng
Ai-ping
AP
Zhu
Wen-xiang
WX
Zhu
Ren-fang
RF
Li
Hong-mei
HM
Zhu
Zhi-dong
ZD
Qin
Ying
Y
Jin
Wei
W
Liu
Yan
Y
Du
Yun-lan
YL
Sun
Jian
J
Xiao
Hua-sheng
HS
D000328:Adult; D000368:Aged; D000483:Alleles; D002681:China / Q000208:ethnology; D019389:Cytochrome P-450 CYP2D6 / Q000235:genetics*; D005260:Female; D018628:Gene Dosage; D006801:Humans; D008297:Male; D008875:Middle Aged
D019389:Cytochrome P-450 CYP2D6