title: Genetic relatedness of Escherichia coli isolates in interstitial water from a Lake Huron (Canada) beach.
abstract: Research was undertaken to characterize Escherichia coli isolates in interstitial water samples of a sandy beach on the southeastern shore of Lake Huron, Ontario, Canada. A survey of the beach area revealed the highest abundance of E. coli in interstitial water of the foreshore beach sand next to the swash zone. Higher concentrations of E. coli (up to 1.6 x 10(6) CFU/100 ml of water) were observed in the interstitial water from the sampling holes on the beach itself compared to lake water and sediment. Repetitive extragenic palindromic PCR (REP-PCR) was used to characterize the genetic diversity of E. coli isolates from interstitial water samples on the beach. E. coli isolates from the same sampling location frequently exhibited the same REP-PCR pattern or were highly similar to each other. In contrast, E. coli isolates from different sampling locations represented populations distinct from each other. This study has identified a unique ecological niche within the foreshore area of the beach where E. coli may survive and possibly multiply outside of host organisms. The results are of interest as increasing concentrations of E. coli in recreational waters are often considered to be an indication of recent fecal pollution.
authors.affiliation: University of Guelph, Guelph, Ontario N1G 2W1, Canada.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Kon
authors.forename: Susan C
authors.initials: SC
authors.lastname: Weir
authors.forename: E Todd
authors.initials: ET
authors.lastname: Howell
authors.forename: Hung
authors.initials: H
authors.lastname: Lee
authors.forename: Jack T
authors.initials: JT
authors.lastname: Trevors
mesh_terms: D015373:Bacterial Typing Techniques; D002170:Canada; D016000:Cluster Analysis; D015169:Colony Count, Microbial; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D021901:DNA, Intergenic / Q000235:genetics; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005618:Fresh Water / Q000382:microbiology*; D014644:Genetic Variation; D005838:Genotype; D019015:Geologic Sediments / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D012988:Soil Microbiology; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D021901:DNA, Intergenic