Microinfusion into the rat brain of antibodies against Semliki Forest Virus produces changes in behavioral response to apomorphine.
Anti-SFV antibodies generated during SFV infection may affect CNS function due to cross-reactivity with a peptide of oligodendrocyte myelin glycoprotein. To explore this possibility, total IgG from SFV immunized or normal control rabbits was unilaterally microinfused into the subthalamic region of normal rat brain. Behavior of the IgG-infused rats was determined using a bioassay, measuring rotational locomotion following systemic injection of apomorphine. Anti-SFV IgG-infused rats demonstrated a significantly increased (p<0.005) ipsilateral turning response compared to control rats, persisting for at least a month. Results suggest that brain cross-reactive antibodies in anti-SFV IgG may affect brain function.
Knopf
Paul M
PM
Department of Molecular Microbiology and Immunology, Brown University Medical School, Division of Biology and Medicine, Brown University, Providence, RI 02912, USA. paul_knopf@brown.edu
Harling-Berg
Christine J
CJ
Lee
Darrin J
DJ
Hallett
Joseph J
JJ
Stopa
Edward G
EG
Mokhtarian
Foroozan
F
D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D001058:Apomorphine / Q000494:pharmacology*; D001522:Behavior, Animal / Q000187:drug effects*; D001921:Brain / Q000187:drug effects; D018491:Dopamine Agonists / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D008297:Male; D009043:Motor Activity / Q000187:drug effects; D051381:Rats; D011916:Rats, Inbred F344; D045442:Rotarod Performance Test; D012672:Semliki forest virus / Q000737:chemistry*; D013997:Time Factors; D014764:Viral Proteins / Q000276:immunology*
D000906:Antibodies; D018491:Dopamine Agonists; D014764:Viral Proteins; D001058:Apomorphine