Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88.
The filamentous fungus Aspergillus niger is widely exploited by the fermentation industry for the production of enzymes and organic acids, particularly citric acid. We sequenced the 33.9-megabase genome of A. niger CBS 513.88, the ancestor of currently used enzyme production strains. A high level of synteny was observed with other aspergilli sequenced. Strong function predictions were made for 6,506 of the 14,165 open reading frames identified. A detailed description of the components of the protein secretion pathway was made and striking differences in the hydrolytic enzyme spectra of aspergilli were observed. A reconstructed metabolic network comprising 1,069 unique reactions illustrates the versatile metabolism of A. niger. Noteworthy is the large number of major facilitator superfamily transporters and fungal zinc binuclear cluster transcription factors, and the presence of putative gene clusters for fumonisin and ochratoxin A synthesis.
Pel
Herman J
HJ
DSM Food Specialties, PO Box 1, 2600 MA Delft, The Netherlands.
de Winde
Johannes H
JH
Archer
David B
DB
Dyer
Paul S
PS
Hofmann
Gerald
G
Schaap
Peter J
PJ
Turner
Geoffrey
G
de Vries
Ronald P
RP
Albang
Richard
R
Albermann
Kaj
K
Andersen
Mikael R
MR
Bendtsen
Jannick D
JD
Benen
Jacques A E
JA
van den Berg
Marco
M
Breestraat
Stefaan
S
Caddick
Mark X
MX
Contreras
Roland
R
Cornell
Michael
M
Coutinho
Pedro M
PM
Danchin
Etienne G J
EG
Debets
Alfons J M
AJ
Dekker
Peter
P
van Dijck
Piet W M
PW
van Dijk
Alard
A
Dijkhuizen
Lubbert
L
Driessen
Arnold J M
AJ
d'Enfert
Christophe
C
Geysens
Steven
S
Goosen
Coenie
C
Groot
Gert S P
GS
de Groot
Piet W J
PW
Guillemette
Thomas
T
Henrissat
Bernard
B
Herweijer
Marga
M
van den Hombergh
Johannes P T W
JP
van den Hondel
Cees A M J J
CA
van der Heijden
Rene T J M
RT
van der Kaaij
Rachel M
RM
Klis
Frans M
FM
Kools
Harrie J
HJ
Kubicek
Christian P
CP
van Kuyk
Patricia A
PA
Lauber
Jürgen
J
Lu
Xin
X
van der Maarel
Marc J E C
MJ
Meulenberg
Rogier
R
Menke
Hildegard
H
Mortimer
Martin A
MA
Nielsen
Jens
J
Oliver
Stephen G
SG
Olsthoorn
Maurien
M
Pal
Karoly
K
van Peij
Noël N M E
NN
Ram
Arthur F J
AF
Rinas
Ursula
U
Roubos
Johannes A
JA
Sagt
Cees M J
CM
Schmoll
Monika
M
Sun
Jibin
J
Ussery
David
D
Varga
Janos
J
Vervecken
Wouter
W
van de Vondervoort
Peter J J
PJ
Wedler
Holger
H
Wösten
Han A B
HA
Zeng
An-Ping
AP
van Ooyen
Albert J J
AJ
Visser
Jaap
J
Stam
Hein
H
D001234:Aspergillus niger / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal / Q000235:genetics*; D016681:Genome, Fungal / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*
D010940:Plant Proteins