[Simultaneous determination of eight organic acids in Fructus mume by RP-HPLC].
OBJECTIVE
To develop an HPLC method for the simultaneous separation and determination of oxalic acid (OA), tartaric acid(TA), malic acid(MA), vitamin C (VC), lactic acid (LA), acetic acid (AA) citric acid (CA) and succinic acid (SA) in Fructus mume.


METHODS
Analytical column was Zorbax Eclipse XDB C18. Mobile phase was 0.5% (NH4) H2PO4 aqueous solution and detection wavelength was 214 nm. The flow rate of mobile phase was 0.5 mL x min(-1).


RESULTS
The regression equations (pH 2. 8, adjusted with phosphoric acid) of eight constituents have been established, r = 0.999 7, 0. 999 8, 0.999 2, 0.999 6, 0.999 1, 0.999 5, 0.999 8, 0.999 2 respectively. Meanwhile, the content and proportion relationship of eight organic acids in Fructus mume which yielded in Fujian (China) were investigated.


CONCLUSIONS
This method was simple, accuracy and quick. The method can be used for the purpose of routine analysis and the quality control of a botanic (Fructus mume) containing these organic acid components.
Chen
Zhan-Guo
ZG
College of Chemistry and Materials Science, Shaaxi Normal University, Xi'an 710062, China. chzhg@snnu.edu.cn
En
Bo-Ti
BT
Zhang
Zhi-Qi
ZQ
D019342:Acetic Acid / Q000032:analysis; D001205:Ascorbic Acid / Q000032:analysis; D002264:Carboxylic Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019343:Citric Acid / Q000032:analysis; D005638:Fruit / Q000737:chemistry; D019344:Lactic Acid / Q000032:analysis; D008293:Malates / Q000032:analysis; D019815:Oxalic Acid / Q000032:analysis; D010946:Plants, Medicinal / Q000737:chemistry*; D027861:Prunus / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D019802:Succinic Acid / Q000032:analysis; D013644:Tartrates / Q000032:analysis
D002264:Carboxylic Acids; D008293:Malates; D013644:Tartrates; D019343:Citric Acid; D019344:Lactic Acid; C030298:malic acid; D019815:Oxalic Acid; D019802:Succinic Acid; D001205:Ascorbic Acid; D019342:Acetic Acid; C029768:tartaric acid