title: Involvement of Candida albicans pyruvate dehydrogenase complex protein X (Pdx1) in filamentation.
abstract: For 50 years, physiologic studies in Candida albicans have associated fermentation with filamentation and respiration with yeast morphology. Analysis of the mitochondrial proteome of a C. albicans NDH51 mutant, known to be defective in filamentation, identified increased expression of several proteins in the respiratory pathway. Most notable was a 15-fold increase in pyruvate dehydrogenase complex protein X (Pdx1), an essential component of the pyruvate dehydrogenase complex. In basal salts medium with < or = 100 mM glucose as carbon source, two independent pdx1 mutants displayed a filamentation defect identical to ndh51; reintegration of one PDX1 allele restored filamentation. Concentrations of glucose < or = 100 mM did not correct the filamentation defect. Expanding on previous work, these studies suggest that increased expression of proteins extraneous to the electron transport chain compensates for defects in the respiratory pathway to maintain yeast morphology. Mitochondrial proteomics can aid in the identification of C. albicans genes not previously implicated in filamentation.
authors.affiliation: Department of Pediatrics and Program in Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06520-8064, USA.
authors.forename: Vincent F
authors.initials: VF
authors.lastname: Vellucci
authors.forename: Scott E
authors.initials: SE
authors.lastname: Gygax
authors.forename: Margaret K
authors.initials: MK
authors.lastname: Hostetter
mesh_terms: D002176:Candida albicans / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D004579:Electron Transport; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D025301:Hyphae / Q000201:enzymology / Q000254:growth & development; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D011768:Pyruvate Dehydrogenase Complex / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D012402:Rotenone / Q000494:pharmacology
chemical_list: D005656:Fungal Proteins; D011768:Pyruvate Dehydrogenase Complex; D012402:Rotenone; D005947:Glucose