title: Dlx homeobox genes promote cortical interneuron migration from the basal forebrain by direct repression of the semaphorin receptor neuropilin-2.
abstract: Dlx homeobox genes play an important role in vertebrate forebrain development. Dlx1/Dlx2 null mice die at birth with an abnormal cortical phenotype, including impaired differentiation and migration of GABAergic interneurons to the neocortex. However, the molecular basis for these defects downstream of loss of Dlx1/Dlx2 function is unknown. Neuropilin-2 (NRP-2) is a receptor for Class III semaphorins, which inhibit neuronal migration. Herein, we show that Neuropilin-2 is a specific DLX1 and DLX2 transcriptional target by applying chromatin immunoprecipitation to embryonic forebrain tissues. Both homeobox proteins repress Nrp-2 expression in vitro, confirming the functional significance of DLX binding. Furthermore, the homeodomain of DLX1 and DLX2 is necessary for DNA binding and this binding is essential for Dlx repression of Nrp-2 expression. Of importance, there is up-regulated and aberrant expression of NRP-2 in the forebrains of Dlx1/Dlx2 null mice. This is the first report that DLX1 or DLX2 can function as transcriptional repressors. Our data show that DLX proteins specifically mediate the repression of Neuropilin-2 in the developing forebrain. As well, our results support the hypothesis that down-regulation of Neuropilin-2 expression may facilitate tangential interneuron migration from the basal forebrain.
authors.affiliation: Department of Biochemistry and Medical Genetics, Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba R3E 0V9, Canada.
authors.forename: Trung N
authors.initials: TN
authors.lastname: Le
authors.forename: Guoyan
authors.initials: G
authors.lastname: Du
authors.forename: Mario
authors.initials: M
authors.lastname: Fonseca
authors.forename: Qing-Ping
authors.initials: QP
authors.lastname: Zhou
authors.forename: Jeffrey T
authors.initials: JT
authors.lastname: Wigle
authors.forename: David D
authors.initials: DD
authors.lastname: Eisenstat
mesh_terms: D000818:Animals; D001483:Base Sequence; D002465:Cell Movement / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism*; D007395:Interneurons / Q000473:pathology* / Q000502:physiology; D051379:Mice; D008818:Mice, Neurologic Mutants; D008969:Molecular Sequence Data; D039943:Neuropilin-2 / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics / Q000502:physiology; D016548:Prosencephalon / Q000002:abnormalities* / Q000473:pathology; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*
chemical_list: C088334:Distal-less homeobox proteins; D018398:Homeodomain Proteins; D039943:Neuropilin-2; D014157:Transcription Factors