Cardiac side population cells have a potential to migrate and differentiate into cardiomyocytes in vitro and in vivo.
Side population (SP) cells, which can be identified by their ability to exclude Hoechst 33342 dye, are one of the candidates for somatic stem cells. Although bone marrow SP cells are known to be long-term repopulating hematopoietic stem cells, there is little information about the characteristics of cardiac SP cells (CSPs). When cultured CSPs from neonatal rat hearts were treated with oxytocin or trichostatin A, some CSPs expressed cardiac-specific genes and proteins and showed spontaneous beating. When green fluorescent protein-positive CSPs were intravenously infused into adult rats, many more ( approximately 12-fold) CSPs were migrated and homed in injured heart than in normal heart. CSPs in injured heart differentiated into cardiomyocytes, endothelial cells, or smooth muscle cells (4.4%, 6.7%, and 29% of total CSP-derived cells, respectively). These results suggest that CSPs are intrinsic cardiac stem cells and involved in the regeneration of diseased hearts.
Oyama
Tomomi
T
Department of Cardiovascular Science and Medicine, Chiba University Graduate School of Medicine, Chuo-ku, Chiba-shi, Chiba 260-8670, Japan.
Nagai
Toshio
T
Wada
Hiroshi
H
Naito
Atsuhiko Thomas
AT
Matsuura
Katsuhisa
K
Iwanaga
Koji
K
Takahashi
Toshinao
T
Goto
Motohiro
M
Mikami
Yoko
Y
Yasuda
Noritaka
N
Akazawa
Hiroshi
H
Uezumi
Akiyoshi
A
Takeda
Shin'ichi
S
Komuro
Issei
I
D017667:Adipocytes / Q000166:cytology; D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000502:physiology; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000235:genetics; D006877:Hydroxamic Acids / Q000494:pharmacology; D066298:In Vitro Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D009206:Myocardium / Q000166:cytology*; D032383:Myocytes, Cardiac / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000166:cytology; D010011:Osteocytes / Q000166:cytology; D010121:Oxytocin / Q000494:pharmacology; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects
D006877:Hydroxamic Acids; D011500:Protein Synthesis Inhibitors; D049452:Green Fluorescent Proteins; C012589:trichostatin A; D010121:Oxytocin