Marinobacter gudaonensis sp. nov., isolated from an oil-polluted saline soil in a Chinese oilfield.
Two novel strains, SL014B61A(T) and SL014B11A, were isolated from an oil-polluted saline soil from Gudao in the coastal Shengli Oilfield, eastern China. Cells of strains SL014B61A(T) and SL014B11A were motile, Gram-negative and rod-shaped. Growth occurred at NaCl concentrations of between 0 and 15 % and at temperatures of between 10 and 45 degrees C. Strain SL014B61A(T) had Q9 as the major respiratory quinone and C16 : 0 (21.2 %), C18 : 1omega9c (20.3 %), C16 : 1omega7c (7.3 %) and C16 : 1omega9c (6.4 %) as predominant fatty acids. The G+C content of the DNA was 57.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SL014B61A(T) belonged to the genus Marinobacter in the class Gammaproteobacteria. Strain SL014B61A(T) showed the highest 16S rRNA gene sequence similarity with Marinobacter bryozoorum (97.9 %) and showed 97.8 % sequence similarity to Marinobacter lipolyticus. DNA-DNA relatedness to the reference strains Marinobacter bryozoorum and Marinobacter lipolyticus was 35.5 % and 33.8 %, respectively. On the basis of these data, it is proposed that strains SL014B61A(T) and SL014B11A represent a novel species, Marinobacter gudaonensis sp. nov. The type strain is strain SL014B61A(T) (=DSM 18066(T)=LMG 23509(T)=CGMCC 1.6294(T)).
Gu
Jun
J
Department of Environmental Science and Engineering, State Joint Key Laboratory of Environmental Simulation and Pollution Control, Tsinghua University, Beijing 100084, China.
Cai
Hua
H
Department of Environmental Science and Engineering, State Joint Key Laboratory of Environmental Simulation and Pollution Control, Tsinghua University, Beijing 100084, China.
Yu
Su-Lin
SL
Department of Environmental Science and Engineering, State Joint Key Laboratory of Environmental Simulation and Pollution Control, Tsinghua University, Beijing 100084, China.
Qu
Ri
R
Department of Environmental Science and Engineering, State Joint Key Laboratory of Environmental Simulation and Pollution Control, Tsinghua University, Beijing 100084, China.
Yin
Bin
B
State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
Guo
Yu-Feng
YF
Department of Environmental Science and Engineering, State Joint Key Laboratory of Environmental Simulation and Pollution Control, Tsinghua University, Beijing 100084, China.
Zhao
Jin-Yi
JY
Daqing Oilfield Company Ltd, Daqing 163712, China.
Wu
Xiao-Lei
XL
Department of Environmental Science and Engineering, State Joint Key Laboratory of Environmental Simulation and Pollution Control, Tsinghua University, Beijing 100084, China.
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004787:Environmental Pollution; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry; D020459:Genes, rRNA; D053520:Marinobacter / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D009068:Movement; D009693:Nucleic Acid Hybridization; D010578:Petroleum; D010802:Phylogeny; D011809:Quinones / Q000032:analysis / Q000737:chemistry; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis*
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010578:Petroleum; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants