Amphiphilic effects of dibucaine HCl on rotational mobility of n-(9-anthroyloxy)stearic acid in neuronal and model membranes.
We studied dibucaine's effects on specific locations of n-(9-anthroyloxy)palmitic acid or stearic acid (n-AS) within phospholipids of synaptosomal plasma membrane vesicles isolated from bovine cerebral cortex (SPMV) and model membranes. Giant unilamellar vesicles (GUVs) were prepared with total lipids (SPMVTL) and mixture of several phospholipids (SPMVPL) extracted from SPMV. Dibucaine.HCl increased rotational mobility (increased disordering) of hydrocarbon interior, but it decreased mobility (increased ordering) of membrane interface, in both native and model membranes. The degree of rotational mobility in accordance with the carbon atom numbers of phospholipids comprising neuronal and model membranes was in the order at the 16, 12, 9, 6 and 2 position of aliphatic chain present in phospholipids. The sensitivity of increasing or decreasing effect of rotational mobility of hydrocarbon interior or surface region by dibucaine.HCl differed depending on the neuronal and model membranes in the descending order of SPMV, SPMVPL and SPMVTL.
Lee
Young-Ho
YH
Department of Dental Pharmacology and Biophysics, College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University, Busan 602-739, Republic of Korea.
Park
No-Soo
NS
Kwon
Jong-Deok
JD
Park
Ju-Seok
JS
Shin
Gye-Beom
GB
Lee
Chung-Seob
CS
Jung
Tae-Sang
TS
Choi
Nak-Jin
NJ
Yoon
Jin-Hyeok
JH
Ok
Jin-Seok
JS
Yoon
Ung-Chan
UC
Bae
Moon-Kyung
MK
Jang
Hye-Ock
HO
Yun
Il
I
D000779:Anesthetics, Local / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D002540:Cerebral Cortex / Q000166:cytology; D003992:Dibucaine / Q000737:chemistry / Q000494:pharmacology*; D006838:Hydrocarbons / Q000737:chemistry; D009474:Neurons / Q000648:ultrastructure; D010743:Phospholipids / Q000737:chemistry; D013229:Stearic Acids / Q000737:chemistry*; D013500:Surface Tension; D013570:Synaptic Membranes / Q000737:chemistry* / Q000187:drug effects*
D000779:Anesthetics, Local; D006838:Hydrocarbons; D010743:Phospholipids; D013229:Stearic Acids; D003992:Dibucaine