Chemical interaction of polyphosphoric acid with titanium and its effect on human bone marrow derived mesenchymal stem cell behavior.
The aim of this study was to evaluate the effect of treating titanium (Ti) with polyphosphoric acid on the attachment and proliferation of human bone marrow derived mesenchymal stem cells (hBMSCs). Cleaned Ti disks were immersed into three different concentrations of polyphosphoric acid solution (0.1, 1, and 10 wt %) and 10 wt % orthophosphoric acid solution for 24 h at 37 degrees C. Ti immersed in distilled water for 24 h at 37 degrees C served as control. The level of polyphosphoric acid that interacted with the Ti surface was determined by measuring the surface P/Ti ratio (atom%/atom%) using X-ray photoelectron spectroscopy. Degrees of cell attachment (1, 3, 5 h after cell seed) and proliferation (1, 3, 5, and 7 days after cell seed) on each treated Ti disk were evaluated by MTS assay. The mean surface P/Ti ratios increased in a polyphosphoric acid concentration dependent manner. A significantly higher cell attachment was found on Ti treated with polyphosphoric acid in contrast to untreated Ti (control) for all three culture periods. MTS assay also revealed that cell proliferation levels significantly increased following a polyphosphoric acid dose dependency. Ti surface treatment with orthophosphoric acid did not influence the cell attachment and proliferation. It was concluded that polyphosphoric acid treatment of Ti enhanced the attachment and proliferation of hBMSCs.
Maekawa
Kenji
K
Department of Oral and Maxillofacial Rehabilitation, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan. maekawa@md.okayama-u.ac.jp
Yoshida
Yasuhiro
Y
Mine
Atsushi
A
Fujisawa
Takuo
T
Van Meerbeek
Bart
B
Suzuki
Kazuomi
K
Kuboki
Takuo
T
D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible; D004347:Drug Interactions; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D010756:Phosphoric Acids / Q000737:chemistry* / Q000494:pharmacology; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D013057:Spectrum Analysis; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology; D014965:X-Rays
D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D010756:Phosphoric Acids; D011108:Polymers; C047269:polyphosphoric acid; D014025:Titanium