OsCSLD1, a cellulose synthase-like D1 gene, is required for root hair morphogenesis in rice.
Root hairs are long tubular outgrowths that form on the surface of specialized epidermal cells. They are required for nutrient and water uptake and interact with the soil microflora. Here we show that the Oryza sativa cellulose synthase-like D1 (OsCSLD1) gene is required for root hair development, as rice (Oryza sativa) mutants that lack OsCSLD1 function develop abnormal root hairs. In these mutants, while hair development is initiated normally, the hairs elongate less than the wild-type hairs and they have kinks and swellings along their length. Because the csld1 mutants develop the same density and number of root hairs along their seminal root as the wild-type plants, we propose that OsCSLD1 function is required for hair elongation but not initiation. Both gene trap expression pattern and in situ hybridization analyses indicate that OsCSLD1 is expressed in only root hair cells. Furthermore, OsCSLD1 is the only member of the four rice CSLD genes that shows root-specific expression. Given that the Arabidopsis (Arabidopsis thaliana) gene KOJAK/AtCSLD3 is required for root hair elongation and is expressed in the root hair, it appears that OsCSLD1 may be the functional ortholog of KOJAK/AtCSLD3 and that these two genes represent the root hair-specific members of this family of proteins. Thus, at least part of the mechanism of root hair morphogenesis in Arabidopsis is conserved in rice.
Kim
Chul Min
CM
Division of Applied Life Science, Plant Molecular Biology and Biotechnology Research Center, Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660-701, Korea.
Park
Sung Han
SH
Je
Byoung Il
BI
Park
Su Hyun
SH
Park
Soon Ju
SJ
Piao
Hai Long
HL
Eun
Moo Young
MY
Dolan
Liam
L
Han
Chang-deok
CD
D001483:Base Sequence; D005966:Glucuronidase / Q000032:analysis; D008969:Molecular Sequence Data; D009024:Morphogenesis / Q000235:genetics*; D016254:Mutagenesis, Insertional; D012275:Oryza / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D010940:Plant Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D018517:Plant Roots / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism
D010940:Plant Proteins; D012333:RNA, Messenger; D005966:Glucuronidase