[Role of Slingshot-1L in peripheral eosinophils of asthmatic patients].
OBJECTIVE
To investigate the expression of Slingshot-1L (SSH-1L) in peripheral blood eosinophils of asthmatic patients.


METHODS
Peripheral vein blood sample of 30 ml was collected from 15 healthy volunteers, 15 asthmatic patients with glucocorticoid treatment and 15 asthmatic patients without the treatment. The eosinophils were isolated, purified and counted for each sample, and SSH-1L/beta-actin gene fragments were amplified simultaneously by RT-PCR for the total RNA. SSH-1L protein was detected by Western blotting from the total protein of the peripheral eosinophils. The expressions of SSH-1L at both mRNA and protein levels are compared between different groups.


RESULTS
SSH-1L/beta-actin ratio significantly increased in untreated patients with asthma attacks in comparison with healthy volunteers (P<0.05), which did not occur in patients treated with glucocorticoids (P>0.05). The optical density of SSH-1L protein significantly increased in untreated asthmatic patients (P<0.05), but not in patients treated with glucocorticoids (P>0.05), as compared with the healthy volunteers.


CONCLUSIONS
Significantly increased SSH-1L expression in peripheral eosinophils may play an important role in the activation and migration of eosinophils.
Zhang
Wei-zhen
WZ
Department of Respiratory Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. zhangweizhen157@126.com
Cai
Shao-xi
SX
Zou
Fei
F
Zhao
Hai-jin
HJ
Li
Wen-jun
WJ
D051339:Actin Depolymerizing Factors / Q000378:metabolism; D000199:Actins / Q000378:metabolism; D000328:Adult; D001249:Asthma / Q000097:blood*; D015153:Blotting, Western; D004804:Eosinophils / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010749:Phosphoprotein Phosphatases / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D051339:Actin Depolymerizing Factors; D000199:Actins; D012333:RNA, Messenger; D010749:Phosphoprotein Phosphatases; C484329:SSH1 protein, human