title: Application of a two-dimensional model for predicting the pressure-flow and compression properties during column packing scale-up.
abstract: A two-dimensional model was formulated to describe the pressure-flow behavior of compressible stationary phases for protein chromatography at different temperatures and column scales. The model was based on the assumption of elastic deformation of the solid phase and steady-state Darcy flow. Using a single fitted value for the empirical modulus parameters, the model was applied to describe the pressure-flow behavior of several adsorbents packed using both fluid flow and mechanical compression. Simulations were in agreement with experimental data and accurately predicted the pressure-flow and compression behavior of three adsorbents over a range of column scales and operating temperatures. Use of the described theoretical model potentially improves the accuracy of the column scale-up process, allowing the use of limited laboratory scale data to predict column performance in large scale applications.
authors.affiliation: Biogen Idec Corporation, Bioprocess Development, 14 Cambridge Center, Cambridge, MA 02142, USA. Justin.mccue@biogenidec.com
authors.forename: Justin T
authors.initials: JT
authors.lastname: McCue
authors.forename: Douglas
authors.initials: D
authors.lastname: Cecchini
authors.forename: Cathy
authors.initials: C
authors.lastname: Chu
authors.forename: Wei-Han
authors.initials: WH
authors.lastname: Liu
authors.forename: Andrew
authors.initials: A
authors.lastname: Spann
mesh_terms: D001274:Atmospheric Pressure; D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods*; D008962:Models, Theoretical