title: Modification of cysteinyl leukotriene receptor expression in capsular contracture: Preliminary results.
abstract: The development of a fibrotic capsule around foreign material in the body is a physiologic reaction undertaken by the body to protect itself from a material it does not recognize. The periprosthetic capsule can pathologically contract, pressing on the implant; it can cause pain, firmness, and sometimes implant extrusion. The pathogenesis of capsular contracture is still unclear, but most reports indicate a multifactorial explanation. The aim of this study is to investigate the role of cysteinyl leukotriene receptors (cysLTR) on the inflammatory cells involved in the development of the capsular contracture. We recruited 20 patients affected by severe capsular contracture (Baker III-IV) and a control group composed of normal patients who had undergone implant substitution. In both groups, we performed a semiquantitative analysis of mRNA encoding for cysLTR1, cysLTR2, tumor necrosis factor-alpha (TNF-alpha) and interleukin 10 (IL-10) on myofibroblasts and macrophages of the periprosthetic capsular tissue. The molecular analysis showed an increase in the cysLTR2, TNF-alpha gene expression but no change in the cysLTR1 and IL-10 genes in patients affected by capsular contracture. These preliminary findings suggest a primary role for cysteinyl leukotrienes in the activation and up-regulation of capsular contraction mechanisms.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Pharmacology Section, Second University of Naples, Naples, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: D'Andrea
authors.forename: Giovanni F
authors.initials: GF
authors.lastname: Nicoletti
authors.forename: Elisa
authors.initials: E
authors.lastname: Grella
authors.forename: Roberto
authors.initials: R
authors.lastname: Grella
authors.forename: Dario
authors.initials: D
authors.lastname: Siniscalco
authors.forename: Carlo
authors.initials: C
authors.lastname: Fuccio
authors.forename: Francesco
authors.initials: F
authors.lastname: Rossi
authors.forename: Sabatino
authors.initials: S
authors.lastname: Maione
authors.forename: Vito
authors.initials: V
authors.lastname: De Novellis
mesh_terms: D000328:Adult; D018427:Breast Implants; D003238:Connective Tissue / Q000473:pathology; D003286:Contracture / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D005260:Female; D005347:Fibroblasts / Q000473:pathology; D005355:Fibrosis / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D005549:Foreign-Body Reaction / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics; D008264:Macrophages / Q000473:pathology; D008565:Membrane Proteins / Q000235:genetics*; D011183:Postoperative Complications / Q000473:pathology* / Q000601:surgery; D012333:RNA, Messenger / Q000235:genetics; D018077:Receptors, Leukotriene / Q000235:genetics*; D012016:Reference Values; D012086:Reoperation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020034:Silicone Gels; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D008565:Membrane Proteins; D012333:RNA, Messenger; D018077:Receptors, Leukotriene; D020034:Silicone Gels; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; C083169:leukotriene D4 receptor