title: Surface modification of Ti45Nb alloy by immobilization of RGD peptide via self assembled monolayer.
abstract: A new low modulus beta Ti-Nb alloy with low elastic modulus and excellent corrosion resistance is currently under consideration as a surgical implant material. The usefulness of such materials can be dramatically enhanced if their surface structure and surface chemistry can be controlled. This control is achieved by attaching a self assembled monolayer (SAM) based on 11-chloroacetyl-1-undecylphosphonic acid, CAUDPA, to the surface and immobilization of a peptide to the monolayer. The SAM is characterized by Fourier Transform Infrared Spectroscopy (FTIR) and X-ray Photoelectron Spectroscopy (XPS) at two different takeoff angles. The CAUDPA molecules were covalently bonded on the substrate in a configuration in which the phosphonic group turns toward the Ti45Nb while the acetyl chloride end group tail turns to the topmost surface. In such configuration sequential in situ reaction is possible by exchange between the chloride and a biological molecule. Such biological molecule is the arginine-glycine-aspartic acid-cysteine, RGDC, small amino acid sequence present in many molecules of the extracellular matrix. Preliminary cell culture in-vitro result shows an improvement of the response of osteoblast cells to Ti45Nb after the peptide immobilization.
authors.affiliation: Faculty of Materials Engineering, Technion, Haifa, Israel.
authors.forename: G
authors.initials: G
authors.lastname: Zorn
authors.forename: I
authors.initials: I
authors.lastname: Gotman
authors.forename: E Y
authors.initials: EY
authors.lastname: Gutmanas
authors.forename: R
authors.initials: R
authors.lastname: Adadi
authors.forename: C N
authors.initials: CN
authors.lastname: Sukenik
mesh_terms: D000327:Adsorption; D000497:Alloys / Q000737:chemistry; D000818:Animals; D000831:Animals, Newborn; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D008422:Materials Testing; D009556:Niobium / Q000737:chemistry*; D009842:Oligopeptides / Q000008:administration & dosage* / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D014025:Titanium / Q000737:chemistry*
chemical_list: D000497:Alloys; D020099:Coated Materials, Biocompatible; D009842:Oligopeptides; D009556:Niobium; C047981:arginyl-glycyl-aspartic acid; D014025:Titanium