Clonal Multiplication of Aconitum carmichaeli by Tip Tissue Culture and Alkaloid Contents of Clonally Propagated Plant.
Shoot tips and axillary buds of ACONITUM CARMICHAELI Debx. cultured in Murashige and Skoog medium supplemented with 5 ppm BAP at 25 +/- 1 degrees C under continuous light for 6 weeks formed multiple shoots with an average of 7 shoots per culture. Multiple shoot forming ability does not decrease at least for 6 generations. Subsequent culture of these shoots on MS medium supplemented with 0.5 ppm IAA at 20 +/- 1 degrees C under continuous light for 6 weeks resulted in root formation. The plantlets were transplanted successfully in the soil. Alkaloid contents of clonally-propagated plants showed less variation than those of the normally grown plants.
Hatano
K
K
Faculty of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812, Japan.
Kamura
K
K
Shoyama
Y
Y
Nishioka
I
I