title: Migration behavior of isoluminol isothiocyanate-labeled alpha-amino acids in capillary electrophoresis with an absorption/chemiluminescence dual detection system.
abstract: In our previous study, we developed capillary electrophoresis with an ultraviolet absorption/chemiluminescence dual detection system. Here, to demonstrate one of the possible applications of the capable system as well as confirm the advantage, migration behavior of isoluminol isothiocyanate-labeled alpha-amino acids was examined in the capillary electrophoresis with a dual detection system. The labeled samples were first analyzed by absorption detection with an on-capillary, followed by chemiluminescence detection with an end-capillary. The system easily, rapidly, and simultaneously produced useful information concerning chemiluminescence quenching and amino group-labeling due to the presence of both absorption and chemiluminescence detections.
authors.affiliation: Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University, Kyotanabe 610-0321, Japan. ktsukago@mail.doshisha.ac.jp
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Tsukagoshi
authors.forename: Kaori
authors.initials: K
authors.lastname: Sawanoi
authors.forename: Riichiro
authors.initials: R
authors.lastname: Nakajima
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D017879:Isothiocyanates / Q000737:chemistry*; D049449:Luminescence; D008165:Luminol / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D000596:Amino Acids; D017879:Isothiocyanates; C032487:isoluminol; D008165:Luminol