In vitro cytotoxic and non-genotoxic effects of gutta-percha solvents on mouse lymphoma cells by single cell gel (comet) assay.
Chloroform and eucalyptol are widely used in clinical dentistry as gutta-percha solvents. However, these compounds may represent a hazard to human health, especially by causing injury to genetic apparatus and/or inducing cellular death. In this study, the genotoxic and cytotoxic potentials associated with exposure to chloroform and eucalyptol were assessed on mouse lymphoma cells in vitro by the single cell gel (comet) assay and trypan blue exclusion test, respectively. Both gutta-percha solvents proved to be cytotoxic at the same levels in concentrations of 2.5, 5 and 10 microL/mL (p<0.05). On the other hand, neither of the solvents induced DNA breakage. Taken together, these results suggest that although both tested compounds (chloroform and eucalyptol) are strong cytotoxicants, it seems that they are not likely to increase the level of DNA damage on mammalian cells.
Ribeiro
Daniel Araki
DA
Center of Genotoxin and Carcinogen Evaluation, Department of Pathology, School of Medicine of Botucatu, Sao Paulo State University, Botucatu, SP, Brazil. araki@fmb.unesp.br
Marques
Mariângela Esther Alencar
ME
Salvador
Daisy Maria Fávero
DM
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002725:Chloroform / Q000008:administration & dosage / Q000633:toxicity*; D004396:Coloring Agents; D020552:Comet Assay; D003511:Cyclohexanols / Q000008:administration & dosage / Q000633:toxicity*; D004247:DNA / Q000187:drug effects; D053960:DNA Breaks; D000077591:Eucalyptol; D005052:Eucalyptus; D006171:Gutta-Percha / Q000737:chemistry; D007940:Leukemia L5178 / Q000473:pathology*; D051379:Mice; D039821:Monoterpenes / Q000008:administration & dosage / Q000633:toxicity*; D009153:Mutagens / Q000633:toxicity; D012997:Solvents / Q000008:administration & dosage / Q000633:toxicity*; D014343:Trypan Blue
D004396:Coloring Agents; D003511:Cyclohexanols; D039821:Monoterpenes; D009153:Mutagens; D012997:Solvents; D002725:Chloroform; D006171:Gutta-Percha; D004247:DNA; D014343:Trypan Blue; D000077591:Eucalyptol