title: Immunohistochemical detection with quantum dots.
abstract: Quantum dot (QD) conjugates have many immunohistochemical applications. The optical, excitation/emission, and photostable properties of QDs offer several advantages over the use of chromogens or organic fluorophores in these applications. Here, we describe the use of QD conjugates to detect primary antibody binding in fixed tissue sections. We also describe the use of QDs in simultaneous and sequential multilabeling procedures and in combination with enzyme-based signal amplification techniques. QD conjugates expand the arsenal of the immunohistochemist and increase experimental flexibility in many applications.
authors.affiliation: Division of Neuropathology, Department of Pathology, University of Alabama at Birmingham, AL, USA.
authors.forename: Rizwan S
authors.initials: RS
authors.lastname: Akhtar
authors.forename: Cecelia B
authors.initials: CB
authors.lastname: Latham
authors.forename: Dario
authors.initials: D
authors.lastname: Siniscalco
authors.forename: Carlo
authors.initials: C
authors.lastname: Fuccio
authors.forename: Kevin A
authors.initials: KA
authors.lastname: Roth
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes / Q000737:chemistry; D007150:Immunohistochemistry; D051379:Mice; D008856:Microscopy, Fluorescence; D045663:Quantum Dots
chemical_list: D000906:Antibodies; D005456:Fluorescent Dyes