title: Clonal propagation of Cnidium officinale by shoot tip culture.
abstract: Multiple shoots were induced from the apical domes of shoot tips of Cnidium officinale Makino (Apiaceae) by culturing them on Murashige and Skoog (MS) 1 static media solidified with 0.2% gelrite and supplemented with 6-benzylaminopurine (BAP) 10 (-6)M. An average of 5.3 shoots per segment were obtained within 6 weeks and this ability did not decline even after two years of subculture. Subsequent transfer of these regenerated shoots on MS media supplemented with alpha-naphthaleneacetic acid (NAA) 10 (-7)M and BAP 10 (-7)M resulted in root formation. Rooted plantlets were able to grow in soil after a short period of acclimatization. Cytological observation in root tip cells of cultivated, as well as in vitro propagated plantlets revealed that in both cases the cells had 2n = 22 chromosomes indicating the homogeneity of the clonally propagated plants.
authors.affiliation: Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine, 1-2-3 Kasumi, Minami-ku, Hiroshima 734, Japan.
authors.forename: B
authors.initials: B
authors.lastname: Pant
authors.forename: H
authors.initials: H
authors.lastname: Kohda
authors.forename: A
authors.initials: A
authors.lastname: Namera