Localization of the single copy gene Mdh2 on Xenopus tropicalis chromosomes by FISH-TSA.
A single copy gene, mitochondrial malate dehydrogenase 2 (Mdh2), was localized on Xenopus tropicalis chromosomes by fluorescence in situ hybridization coupled with tyramide signal amplification (FISH-TSA). The respective cDNA was cloned and sequenced. The labeled probe hybridized with a subcentromeric region of the long arms of homologous chromosomes 3. Results of comparison of the gene localization with previously mapped X. laevis paralogs strongly suggest a common evolutionary origin of chromosomes 3 and 8 in X. laevis and chromosome 3 in X. tropicalis. This is the first time that a single copy gene has been visualized on X. tropicalis chromosomes. The FISH-TSA method gives a strong signal with a 1-kb labeled probe.
Krylov
V
V
Charles University in Prague, Faculty of Science, Department of Physiology and Developmental Biology, Prague, Czech Republic. vkrylov@natur.cuni.cz
Tlapakova
T
T
Macha
J
J
D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D008040:Genetic Linkage; D006824:Hybridization, Genetic; D017404:In Situ Hybridization, Fluorescence; D008291:Malate Dehydrogenase / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D017386:Sequence Homology, Amino Acid; D014981:Xenopus / Q000235:genetics*; D029867:Xenopus Proteins / Q000235:genetics*; D014982:Xenopus laevis
D018076:DNA, Complementary; D029867:Xenopus Proteins; D008291:Malate Dehydrogenase