title: Detection of infectious parasites in reclaimed water.
abstract: The presence of infectious protozoan pathogens in reclaimed water may present an unacceptable health risk. This study was designed similar to a study reported by Garcia et al. (2002), which detected no infectious Giardia cysts in the final effluent of a tertiary treatment facility as determined by animal infectivity (dose 1000 cysts/gerbil). This study also included evaluation of Cryptosporidium oocyst infectivity. Infectious Giardia cysts were detected in the final effluent with 1 gerbil out of 3 inoculated with 250 cysts from reclaimed water showing signs of infection 15 days postinoculation. None of the Cryptosporidium oocysts concentrated from the reclaimed water samples appeared to be infectious.
authors.affiliation: University of South Florida, College of Marine Science, St Petersburg 33701, USA. dhuffman@seas.marine.usf.edu
authors.forename: Debra E
authors.initials: DE
authors.lastname: Huffman
authors.forename: Angela L
authors.initials: AL
authors.lastname: Gennaccaro
authors.forename: Tracy L
authors.initials: TL
authors.lastname: Berg
authors.forename: Glenda
authors.initials: G
authors.lastname: Batzer
authors.forename: Giovanni
authors.initials: G
authors.lastname: Widmer
mesh_terms: D000818:Animals; D003458:Cryptosporidium / Q000302:isolation & purification* / Q000472:pathogenicity; D005849:Gerbillinae; D005872:Giardia / Q000302:isolation & purification* / Q000472:pathogenicity; D033661:Oocysts; D014865:Waste Disposal, Fluid; D014871:Water Microbiology