title: Efficacy of Sargassum polycystum (Phaeophyceae) sulphated polysaccharide against paracetamol-induced DNA fragmentation and modulation of membrane-bound phosphatases during toxic hepatitis.
abstract: 1. The aim of the present study was to assess the protective effect of Sargassum polycystum (sulphated polysaccharide) extract against paracetamol-induced DNA strand breaks and modulation of membrane-bound phosphatases, protein thiols and inorganic cations during toxic hepatitis. 2. Seaweed extract (200 mg/kg per day for 21 days) was administered to male Wistar rats against paracetamol challenge. Serum and liver tissues were used to assess levels of ATPase, protein thiols and inorganic cations using atomic absorption spectroscopy. The fragmentation of DNA was assessed by agarose gel electrophoresis. 3. Paracetamol induced intracellular stress, accompanied by changes in the structural and functional characteristics of liver cell membranes, which affected DNA integrity, membrane-bound ATPase and inorganic cations homeostasis. Rats intoxicated with paracetamol (800 mg/kg, i.p.) showed significant impairment in activities of total ATPase, Mg2+-ATPase, Ca+-ATPase and Na+/K+-ATPase, with concomitant changes in the levels of tissue protein thiols and inorganic cations, such as Na+, K+ and Ca2+. These changes were prevented in animals pretreated with S. polycystum extract, which indicates that S. polycystum supplementation could exert some protective effect against paracetamol-induced toxic hepatitis in rats. 4. The protective effect of the seaweed extract may be due to the presence of sulphated compounds that have free radical-scavenging activity.
authors.affiliation: Department of Biochemistry, University of Madras, Guindy Campus, Chennai, Tamilnadu, India.
authors.forename: H B
authors.initials: HB
authors.lastname: Raghavendran
authors.forename: A
authors.initials: A
authors.lastname: Sathivel
authors.forename: R S S K
authors.initials: RS
authors.lastname: Yogeeta
authors.forename: T
authors.initials: T
authors.lastname: Devaki
mesh_terms: D000082:Acetaminophen / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D000975:Antioxidants; D002412:Cations / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000201:enzymology / Q000473:pathology*; D004247:DNA / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects*; D006651:Histocytochemistry; D008099:Liver / Q000201:enzymology; D008297:Male; D008566:Membranes / Q000201:enzymology; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D011134:Polysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D044647:Sargassum / Q000737:chemistry* / Q000166:cytology; D013054:Spectrophotometry, Atomic; D013431:Sulfates; D013438:Sulfhydryl Compounds / Q000378:metabolism
chemical_list: D000975:Antioxidants; D002412:Cations; D011134:Polysaccharides; D013431:Sulfates; D013438:Sulfhydryl Compounds; D000082:Acetaminophen; D004247:DNA; D010744:Phosphoric Monoester Hydrolases; D000251:Adenosine Triphosphatases