title: Analysis of several iridoid and indole precursors of terpenoid indole alkaloids with a single HPLC run.
abstract: An isocratic HPLC system is described which allows the separation of the iridoid and indole precursors of terpenoid indole alkaloids, which are present in a single crude extract. The system consists of a column of LiChrospher 60 RP select B 5 microm, 250 x 4 mm (Merck) with an eluent of 1% formic acid-acetonitrile-trichloroacetic acid (100:10:0.25, v:v:w) at a flow of 1.2 ml/min. In the suspension cultures of Catharanthus roseus secologanin and tryptophan were detected. in the cultures of Tabernaemontana divaricata loganin, tryptophan, and tryptamine accumulated.
authors.affiliation: Division of Pharmacognosy, Center for Biopharmaceutical Sciences, Leiden University, The Netherlands.
authors.forename: D
authors.initials: D
authors.lastname: Dagnino
authors.forename: J
authors.initials: J
authors.lastname: Schripsema
authors.forename: R
authors.initials: R
authors.lastname: Verpoorte