An siRNA-based system for differential regulation of ectopic gene expression constructs.
We describe an siRNA (short interfering RNA)-based approach for temporary and reversible regulation of engineered expression constructs in cells. Control of cloned genes can be achieved by cotransfection of unique siRNAs, complementary to artificial target sequences, which are integral parts of an expression vector. Application of this method allows simultaneous or mutual-differential regulation of two or more gene constructs within the same cell, reducing unwanted side effects. This method provides several advantages over promoter regulatory systems employing chemical compounds.
Hahn
Peter
P
QIAGEN GmbH, Qiagen Strasse 1, 40724 Hilden, Germany.
Niemann
Gesa
G
Grewe
Anja
A
Bielke
Wolfgang
W
D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D020868:Gene Silencing; D049452:Green Fluorescent Proteins / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection; D000097573:Red Fluorescent Protein
D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D034741:RNA, Small Interfering; D000097573:Red Fluorescent Protein