Optical monitoring of single cells using quantum dots.
Quantum dots (QDs) can be used to label live cells for long-term tracking experiments. The stability of the QDs ensures low toxicity and long-term imaging capability. The multicolor capabilities of QDs allows the simultaneous monitoring of complex cellular populations, allowing more information-rich experiments to be performed. This chapter discusses the ways in which one can label cells with QDs, and a number of ways to characterize the impact that the labeling has on the cellular populations.
Jaiswal
Jyoti K
JK
Department of Cellular Biophysics, The Rockefeller University, New York, NY, USA.
Simon
Sanford M
SM
D001710:Biotin / Q000737:chemistry / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D045663:Quantum Dots; D011990:Receptors, Transferrin / Q000737:chemistry / Q000378:metabolism
D011990:Receptors, Transferrin; D001710:Biotin