title: The locus encoding an oligomorphic family of MHC-A alleles (Mane-A*06/Mamu-A*05) is present at high frequency in several macaque species.
abstract: Several macaques species are used for HIV pathogenesis and vaccine studies, and the characterization of their major histocompatibility complex (MHC) class I genes is required to rigorously evaluate the cellular immune responses induced after immunization and/or infection. In this study, we demonstrate that the gene expressing the Mane-A*06 allele of pig-tailed macaques is an orthologue of the locus encoding the Mamu-A*05 allele family in rhesus macaques. Analysis of the distribution of this locus in a cohort of 63 pig-tailed macaques revealed that it encodes an oligomorphic family of alleles, highly prevalent (90%) in the pig-tailed macaque population. Similarly, this locus was very frequently found (62%) in a cohort of 80 Indian rhesus macaques. An orthologous gene was also detected in cynomolgus monkeys originating from four different geographical locations, but was absent in two African monkey species. Expression analysis in pig-tailed macaques revealed that the Mane-A*06 alleles encoded by this locus are transcribed at 10- to 20-fold lower levels than other MHC-A alleles (Mane-A*03 or Mane-A*10). Despite their conservation and high prevalence among Asian macaque species, the alleles of the Mane-A*06 family and, by extension their orthologues in rhesus and cynomolgus monkeys, may only modestly contribute to cellular immune responses in macaques because of their low level of expression.
authors.affiliation: Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Bernard A P
authors.initials: BA
authors.lastname: Lafont
authors.forename: Christopher M
authors.initials: CM
authors.lastname: McGraw
authors.forename: Sabriya A
authors.initials: SA
authors.lastname: Stukes
authors.forename: Alicia
authors.initials: A
authors.lastname: Buckler-White
authors.forename: Ronald J
authors.initials: RJ
authors.lastname: Plishka
authors.forename: Russell A
authors.initials: RA
authors.lastname: Byrum
authors.forename: Vanessa M
authors.initials: VM
authors.lastname: Hirsch
authors.forename: Malcolm A
authors.initials: MA
authors.lastname: Martin
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019143:Evolution, Molecular; D020440:Gene Duplication; D005787:Gene Frequency; D005805:Genes, MHC Class I; D007438:Introns; D008251:Macaca / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic; D017386:Sequence Homology, Amino Acid