title: HLA class II allele and haplotype frequencies in iranian patients with acute myelogenous leukemia and control group.
abstract: Previous studies have demonstrated some significant differences in HLA allele frequencies in leukemic patients and normal subjects. We have analyzed HLA class II alleles and haplotypes in 60 Iranian patients with acute myelogenous leukemia (AML) and 180 unrelated normal subjects. Blood samples were collected after obtaining informed consents. From the patients and control DNA extraction and HLA typing were performed using PCR-SSP method. Significant positive association with the disease was found for HLA-DRB1*11 allele (35% vs. 24.7%, p=0.033). Two alleles including HLA-DRB4 and -DQB1*0303 were found to be significantly decreased in patients compared to controls. Regarding haplotype analysis, no significant association was found between case and control groups. It is suggested that HLA-DRB1*11 allele plays as a presumptive predisposing factor while the HLA-DRB4 and -DQB1*0303 alleles are suggested as protective genetic factors against acute myelogenous leukemia. Larger studies are needed to confirm and establish the role of these associations with acute myelogenous leukemia.
authors.affiliation: Department of Immunology, Medical School, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Abdolfattah
authors.initials: A
authors.lastname: Sarafnejad
authors.forename: Farideh
authors.initials: F
authors.lastname: Khosravi
authors.forename: Kamran
authors.initials: K
authors.lastname: Alimoghadam
authors.forename: Saied
authors.initials: S
authors.lastname: Dianat
authors.forename: Bita
authors.initials: B
authors.lastname: Ansaripour
authors.forename: Batoul
authors.initials: B
authors.lastname: Moradi
authors.forename: Shahin
authors.initials: S
authors.lastname: Dorkhosh
authors.forename: Aliakbar
authors.initials: A
authors.lastname: Amirzargar
mesh_terms: D016022:Case-Control Studies; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006683:HLA-DQ Antigens / Q000235:genetics*; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; D006684:HLA-DR Antigens / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D007492:Iran / Q000453:epidemiology; D015470:Leukemia, Myeloid, Acute / Q000453:epidemiology / Q000235:genetics*
chemical_list: D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; C069050:HLA-DQA1 antigen; C069051:HLA-DQB1 antigen; D006684:HLA-DR Antigens