title: Susceptibility of Neisseria gonorrhoeae to antimicrobial peptides from amphibian skin, dermaseptin, and derivatives.
abstract: We evaluated the antimicrobial effect of antimicrobial peptides from frog skin belonging to the dermaseptin family against reference and clinical Neisseria gonorrhoeae strains, including penicillin-resistant strains. Dermaseptin S4 exhibited anti-N. gonorrhoeae activity against all strains with MICs ranging between 10 and 100 microg/mL. We then used derivatives of DS4 and determined the anti-N. gonorrhoeae activity of each of analogs. All the derivatives showed antimicrobial activity. Among the different molecules tested, we found that dermaseptins K4S4 (1-16)a and K4S4 (1-28) were the more potent to inhibit N. gonorrhoeae growth with MIC of 10 microg/mL against all strains.
authors.affiliation: Laboratory of Biochemistry (UR/08-45), Faculty of Medicine, 4002 Sousse, Tunisia.
authors.forename: Amira
authors.initials: A
authors.lastname: Zairi
authors.forename: Frédéric
authors.initials: F
authors.lastname: Tangy
authors.forename: Magaly
authors.initials: M
authors.lastname: Ducos-Galand
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Alonso
authors.forename: Khaled
authors.initials: K
authors.lastname: Hani
mesh_terms: D000595:Amino Acid Sequence; D029845:Amphibian Proteins / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000138:chemical synthesis / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009344:Neisseria gonorrhoeae / Q000187:drug effects*; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000494:pharmacology*; D010455:Peptides / Q000138:chemical synthesis
chemical_list: D029845:Amphibian Proteins; D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D010446:Peptide Fragments; D010455:Peptides; C520531:dermaseptin K4S4 (1-16)a; C520530:dermaseptin K4S4 (1-28); C070905:dermaseptin