DNA-fingerprinting (AFLP and RFLP) for genotypic identification in species of the Pleurotus eryngii complex.
Wild populations of edible species are important source of genetic variability for cultivated lines that can undergo a drastic loss of diversity resulting from man's selection. The development of tools aimed at the clear-cut and safe identification and assessment of genetic variability of the wild and cultivated strains is thus a fundamental goal of molecular genetic research. In this study, we used two polymerase chain reaction (PCR)-based fingerprinting methods-amplified fragment length polymorphism (AFLP) and restriction fragment length polymorphism (RFLP) of laccase and manganese peroxidase genes-to assess genetic differences among strains and independently evolving lineages belonging to the Pleurotus eryngii complex. Both laccase RFLP and AFLP have been proved to distinguish unambiguously the three taxa studied: Pleurotus ferulae, P. eryngii, and P. eryngii var. nebrodensis. AFLP also showed enough sensitivity to detect polymorphisms among the strains, proving to be an efficient DNA fingerprinting tool in studies of strain assignment. The divergent RFLP laccase and manganese peroxidase patterns are also discussed in relation to the role played by these genes in the interaction between these fungi and their host plants.
Urbanelli
S
S
Department of Genetics and Molecular Biology, University "La Sapienza" of Rome, Via dei Sardi 70, 00185, Rome, Italy. sandra.urbanelli@uniroma1.it
Della Rosa
V
V
Punelli
F
F
Porretta
D
D
Reverberi
M
M
Fabbri
A A
AA
Fanelli
C
C
D016000:Cluster Analysis; D016172:DNA Fingerprinting / Q000379:methods*; D004271:DNA, Fungal / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics; D005838:Genotype; D007062:Identification, Psychological; D042845:Laccase / Q000235:genetics; D010544:Peroxidases / Q000235:genetics; D020076:Pleurotus / Q000145:classification* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity
D004271:DNA, Fungal; D005656:Fungal Proteins; D042845:Laccase; D010544:Peroxidases; C051129:manganese peroxidase