Role of anti-oxidant enzymes superoxide dismutase and catalase in the development of cataract : study of serum levels in patients with senile and diabetic cataracts.
Cataract is a universal phenomenon in old age and the most common cause of blindness worldover. Oxidative stress is believed to be a key factor in the gradual loss of transparency of lens. Free radicals formed result in derangement and opacification of lens fibres. Superoxide dismutase, catalase and glutathione peroxidase are major anti-oxidant enzymes, which protect against free radicals. The purpose of this study is to evaluate the role of anti-oxidant enzymes - superoxide dismutase and catalase in the development of cataract in senile and diabetic persons. Serum levels of major anti-oxidant enzymes-superoxide dismutase and catalase were estimated in 20 patients with diabetic cataract, and matched with another 20 patients of senile cataract taken as control. Estimation of superoxide dismutase was done by improved spectrophotometric assay based on epinephrine auto-oxidation at 480nm, while catalase estimation was done by the method of Hugo Aebi. The mean serum levels of superoxide dismutase and catalase were lower as age increased. These enzymes were also significantly lower in diabetic cataracts (9.13 and 16.42 units/ml) as compared to senile cataracts (25.30 and 57.27units/ml). Oxidative stress is one of the major factors contributing to cataract formation. In diabetics where there is increased oxidative stress, the serum levels of the major anti-oxidant enzymes decrease, which lead to early cataract formation. It may be concluded that oxidative stress is an important factor in the development of diabetic cataracts and anti-oxidants may have a role in decreasing the incidence of cataract.
Maurya
O P S
OP
Department of Ophthalmology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005.
Mohanty
Lipa
L
Bhaduri
Gautam
G
Chandra
Abhishek
A
D000368:Aged; D000544:Alzheimer Disease / Q000097:blood*; D016022:Case-Control Studies; D002374:Catalase / Q000097:blood*; D002386:Cataract / Q000201:enzymology*; D048909:Diabetes Complications; D003920:Diabetes Mellitus / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000097:blood*
D002374:Catalase; D013482:Superoxide Dismutase