MPSS profiling of embryonic gonad and primordial germ cells in chicken.
The massively parallel signature sequencing (MPSS) provides a greater depth of coverage than expressed sequence tag scan or microarray and provides a comprehensive expression profile. We used the MPSS technology to uncover gene expression profiling in the early embryonic gonads and primordial germ cells (PGCs) in the chicken. Total numbers of sequenced signatures were 1,012,533 and 995,676 for the PGCs and gonad, respectively. Using a noise distribution model, we found that 1.67% of all signatures are expressed at a higher level in PCGs and 2.81% of all signatures are expressed at a higher level in the gonad. The MPSS data are presented via an interactive web interface available at http://snugenome.snu.ac.kr/MPSS. The MPSS data have been submitted to the Gene Expression Omnibus of the National Center for Biotechnology Information (accession number GSM137300 and GSM137301 for PGCs and gonad, respectively).
Kim
Heebal
H
Department of Agricultural Biotechnology, Seoul National University, Seoul, Korea.
Park
Tae Sub
TS
Lee
Woon Kyu
WK
Moon
Sunjin
S
Kim
Jin Nam
JN
Shin
Ji Hye
JH
Jung
Jin Gyoung
JG
Lee
Seon Duk
SD
Park
Sang Hyun
SH
Park
Kyung Je
KJ
Kim
Mi A
MA
Shin
Sang Su
SS
Kim
Tae Min
TM
Nam
Jungrye
J
Kang
Yeonkyung
Y
Lim
Jeong Mook
JM
Han
Jae Yong
JY
D017398:Alternative Splicing / Q000235:genetics; D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D002874:Chromosome Mapping; D030541:Databases, Genetic; D020869:Gene Expression Profiling / Q000379:methods*; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D005854:Germ Cells / Q000378:metabolism*; D006066:Gonads / Q000196:embryology* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D017422:Sequence Analysis, DNA / Q000379:methods*
D012333:RNA, Messenger