Targeted deletion of p97 (VCP/CDC48) in mouse results in early embryonic lethality.
The highly conserved AAA ATPase p97 (VCP/CDC48) has well-established roles in cell cycle progression, proteasome degradation and membrane dynamics. Gene disruption in Saccromyces cerevisiae, Drosophila melanogaster and Trypanosoma brucei demonstrated that p97 is essential in unicellular and multicellular organisms. To explore the requirement for p97 in mammalian cell function and embryogenesis, we disrupted the p97 locus by gene targeting. Heterozygous p97+/- mice were indistinguishable from their wild-type littermates, whereas homozygous mutants did not survive to birth and died at a peri-implantation stage. These results show that p97 is an essential gene for early mouse development.
Müller
J M M
JM
Endothelial Cell Biology Laboratory, Cancer Research UK, 44 Lincoln's Inn Fields, London WC2A 3PX, UK.
Deinhardt
K
K
Rosewell
I
I
Warren
G
G
Shima
D T
DT
D000251:Adenosine Triphosphatases / Q000172:deficiency* / Q000235:genetics* / Q000502:physiology; D000818:Animals; D001947:Breeding; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000502:physiology; D010064:Embryo Implantation / Q000235:genetics; D020964:Embryo Loss / Q000235:genetics*; D053595:Embryonic Stem Cells; D005260:Female; D017353:Gene Deletion; D018390:Gene Targeting; D006579:Heterozygote; D006720:Homozygote; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D018345:Mice, Knockout; D009687:Nuclear Proteins / Q000172:deficiency* / Q000235:genetics* / Q000502:physiology; D000074405:Valosin Containing Protein
D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; D000251:Adenosine Triphosphatases; C404942:p97 ATPase; D000074405:Valosin Containing Protein; C000614179:Vcp protein, mouse