title: Phenotypic transformation including host-range transition through superinfection of T-even phages.
abstract: Mosaic genome design, considered evidence of horizontal gene transfer, is prominent in T-even phage tail fiber genes involved in host recognition. The possibility of direct gene transfer was assessed through superinfection with two virulent phages T2 and PP01, which caused host recognition shift. Two recombinant phages designated as TPr03 and TPr04 were isolated. PCR-restriction fragment length polymorphism analysis and sequence analysis suggested that 18% of the TPr03 and 38% of the TPr04 genome derived from PP01. Both isolates showed host ranges identical to PP01. The results suggested the possibility of generating various recombinant phages by intentional dual infections and of the occasional occurrence in nature of generation of phage showing new characteristics through superinfection, followed by the genomic recombination.
authors.affiliation: Department of Bioengineering, Tokyo Institute of Technology, Nagatsuta-cho, Midori-ku, Yokohama, Japan.
authors.forename: Michiharu
authors.initials: M
authors.lastname: Abe
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Izumoji
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Tanji
mesh_terms: D004926:Escherichia coli / Q000145:classification / Q000821:virology*; D022761:Gene Transfer, Horizontal; D016679:Genome, Viral; D010641:Phenotype; D013604:T-Phages / Q000235:genetics* / Q000472:pathogenicity