Production of poly(3-hydroxybutyric-co-3-hydroxyvaleric acid) having a high hydroxyvalerate content with valeric acid feeding.
The capability of different organic acids to produce a derivative of PHB [poly(3-hydroxybutyric-co-3-hydroxyvaleric acid), P(3HB-co-3HV)] was examined in shake flask cultivations. Propionic and valeric acids demonstrated the potential to produce P(3HB-co-3HV) under nitrogen limiting conditions at 30 degrees C. The addition time and the initial concentration of valeric acid needed for a high cellular HV content were identified by extensive experimentation. Fed-batch cultivation in 7-l bioreactor with valeric acid feeding resulted in the production of PHA containing 54% HV units.
Khanna
Shilpi
S
Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi, 110016, India. ashokiitd@hotmail.com
Srivastava
Ashok K
AK
D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D003470:Culture Media; D049921:Cupriavidus / Q000254:growth & development / Q000378:metabolism*; D010421:Pentanoic Acids / Q000378:metabolism*; D011091:Polyesters / Q000737:chemistry / Q000378:metabolism*; D011422:Propionates / Q000378:metabolism; D000094122:Polyhydroxybutyrates
D003470:Culture Media; D010421:Pentanoic Acids; D011091:Polyesters; D011422:Propionates; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C038780:n-pentanoic acid; C029658:propionic acid