[Study on influence of buffer system on separate capacity of macroporous resin on ferulic liposome].
OBJECTIVE
To study the effect of buffer on separate capacity of macroporous resin. To evaluate the quality of ferulic acid liposome and determine its entrapment efficiency.


METHODS
Different type of macroporous resin counterpoised by buffer system of Na2 HPO3-NaH2, PO3 was used to separate the free ferulic acid from the preparation and HPLC was used to determine the concentration of the ferulic acid to calculate the entrapment efficiency.


RESULTS
This method had good linearity in the range of 0.56 - 2.8 g x mL(-1) (r = 0.999 6). The precision RSD was less than 1.1%. The adsorption effect of macroporous resin on liposome was reduced while it had no effect on the absorption ability of macroporous resin on the ferulic acid by the usage of buffer. The recovery of HPD450 resin on blank liposome was between 97.2% - 100.8%, while the average recovery is 98.1%.


CONCLUSIONS
Buffer system can enhance the separate ability of macroporous resin on liposome and free drug.
Chen
Bao-Yu
BY
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
Qin
Jing
J
Chen
Da-Wei
DW
Hu
Hai-Yang
HY
Yin
Ya-Shu
YS
Yang
Kai-Liang
KL
Luo
Yi-Fan
YF
D000327:Adsorption; D002021:Buffers; D003373:Coumaric Acids / Q000008:administration & dosage* / Q000032:analysis; D004337:Drug Carriers; D008081:Liposomes; D011786:Quality Control; D012117:Resins, Synthetic
D002021:Buffers; D003373:Coumaric Acids; D004337:Drug Carriers; D008081:Liposomes; D012117:Resins, Synthetic; C004999:ferulic acid