Nitrate reductase-mediated synthesis of silver nanoparticles from AgNO3.
Synthesis of silver nanoparticles using alpha-NADPH-dependent nitrate reductase and phytochelatin in vitro has been demonstrated for the first time. The silver ions were reduced in the presence of nitrate reductase, leading to the formation of a stable silver hydrosol 10-25 nm diam. and stabilized by the capping peptide. The nanoparticles were characterized by X-ray diffraction, transmission electron microscopy, X-ray photoelectron spectroscopy and UV-Vis absorption. These studies will help in designing a rational enzymatic strategy for the synthesis of nanomaterials of different chemical composition, shapes and sizes as well as their separation.
Anil Kumar
S
S
Biochemical Sciences Division, National Chemical Laboratory, Pune, India.
Abyaneh
Majid Kazemian
MK
Gosavi
S W
SW
Kulkarni
Sulabha K
SK
Pasricha
Renu
R
Ahmad
Absar
A
Khan
M I
MI
D003460:Crystallization / Q000379:methods; D005670:Fusarium / Q000201:enzymology*; D008968:Molecular Conformation; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods*; D050901:Nitrate Reductase / Q000737:chemistry*; D010316:Particle Size; D012834:Silver / Q000737:chemistry*; D012835:Silver Nitrate / Q000737:chemistry*
D012834:Silver; D012835:Silver Nitrate; D050901:Nitrate Reductase