Rabbit IgG antibodies against phospholipase A2 from Crotalus durissus terrificus neutralize the lethal activity of the venom.
Crotalus durissus terrificus (C.d.t.) (South American rattlesnake) venom possesses myotoxic and neurotoxic activities, both of which are also expressed by crotoxin, the principal toxin of this venom. Crotoxin contains a basic phospholipase A2 (PLA2) and a non toxic acidic protein, crotapotin. We have produced and investigated the ability of IgG antibodies raised in rabbits against PLA2 to neutralize the lethality of the whole venom. PLA2 was isolated by gel filtration chromatography (Sephadex G-75). Specific antibodies were obtained by subcutaneous and intramuscular inoculation of PLA2 (700 microg) with Freund adjuvant. Groups of six mice (20 + 2 g) were inoculated with 0.5 ml i.p. of C. d t. venom (4 microg) or a mixture of venom that had been preincubated with the desired volume of IgG antibodies. Mortality, recorded 24 and 48 h after inoculation, showed that IgG anti-PLA2 were more effective than anticrotalic serum in neutralizing the lethal activity. These results demonstrate that it could be possible to obtain an anti-venom made by specific antibodies with a high level of protection against the lethal component of C.d.t. venom, and/or the inclusion of these antibodies as a supplement in heterologous anti-venoms.
Rodriguez
Juan P
JP
Cátedra de Química Biológica I, Facultad de Ciencias Exactas y Naturales y Agrimensura, Universidad Nacional del Nordeste, Corrientes, Argentina.
De Marzi
Mauricio
M
Maruñak
Silvana
S
Malchiodi
Emilio L
EL
Leiva
Laura C
LC
Acosta
Ofelia
O
D000818:Animals; D000918:Antibody Specificity; D000997:Antivenins / Q000096:biosynthesis / Q000276:immunology* / Q000494:pharmacology; D002021:Buffers; D017839:Crotalus / Q000276:immunology*; D003439:Crotoxin / Q000037:antagonists & inhibitors*; D006461:Hemolysis / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology* / Q000494:pharmacology; D008297:Male; D051379:Mice; D019148:Neuromuscular Blockade; D009500:Neutralization Tests / Q000379:methods*; D010741:Phospholipases A / Q000276:immunology* / Q000302:isolation & purification; D054467:Phospholipases A2; D011817:Rabbits
D000997:Antivenins; D002021:Buffers; D007074:Immunoglobulin G; D003439:Crotoxin; D010741:Phospholipases A; D054467:Phospholipases A2