Electrochemical detection in a microfluidic device of oxidative stress generated by macrophage cells.
The release of reactive oxygen species (ROS) or reactive nitrogen species (RNS), i.e., the initial phase of oxidative stress, by macrophage cells has been studied by electrochemistry within a microfluidic device. Macrophages were first cultured into a detection chamber containing the three electrodes system and were subsequently stimulated by the microinjection of a calcium ionophore (A23187). Their production of ROS and RNS was then measured by amperometry at the surface of a platinized microelectrode. The fabricated microfluidic device provides an accurate measurement of oxidative release kinetics with an excellent reproducibility. We believe that such a method is simple and versatile for a number of advanced applications based on the detection of biological processes of secretion by a few or even a single living cell.
Amatore
Christian
C
Ecole Normale Supérieure, Département de Chimie, UMR CNRS-ENS-UPMC 8640 "PASTEUR", 24 rue Lhomond, Paris Cedex 05, 75231, France.
Arbault
Stéphane
S
Chen
Yong
Y
Crozatier
Cécile
C
Tapsoba
Issa
I
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002138:Calibration; D002460:Cell Line; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D005609:Free Radicals; D007476:Ionophores / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D018384:Oxidative Stress; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D013997:Time Factors
D005609:Free Radicals; D007476:Ionophores; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D000001:Calcimycin