A microfluidic device for practical label-free CD4(+) T cell counting of HIV-infected subjects.
Practical HIV diagnostics are urgently needed in resource-limited settings. While HIV infection can be diagnosed using simple, rapid, lateral flow immunoassays, HIV disease staging and treatment monitoring require accurate counting of a particular white blood cell subset, the CD4(+) T lymphocyte. To address the limitations of current expensive, technically demanding and/or time-consuming approaches, we have developed a simple CD4 counting microfluidic device. This device uses cell affinity chromatography operated under differential shear flow to specifically isolate CD4(+) T lymphocytes with high efficiency directly from 10 microliters of unprocessed, unlabeled whole blood. CD4 counts are obtained under an optical microscope in a rapid, simple and label-free fashion. CD4 counts determined in our device matched measurements by conventional flow cytometry among HIV-positive subjects over a wide range of absolute CD4 counts (R(2) = 0.93). This CD4 counting microdevice can be used for simple, rapid and affordable CD4 counting in point-of-care and resource-limited settings.
Cheng
Xuanhong
X
Surgical Services and Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, and Shriners Hospital for Children, Boston, Massachusetts 02114, USA.
Irimia
Daniel
D
Dixon
Meredith
M
Sekine
Kazuhiko
K
Demirci
Utkan
U
Zamir
Lee
L
Tompkins
Ronald G
RG
Rodriguez
William
W
Toner
Mehmet
M
D015704:CD4 Antigens / Q000096:biosynthesis; D018791:CD4 Lymphocyte Count / Q000379:methods*; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism* / Q000821:virology; D002448:Cell Adhesion; D002846:Chromatography, Affinity; D005434:Flow Cytometry / Q000379:methods; D015658:HIV Infections / Q000097:blood*; D006679:HIV Seropositivity / Q000097:blood*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D013499:Surface Properties; D013997:Time Factors
D015704:CD4 Antigens