title: The effects of airway pressure and inspiratory time on bacterial translocation.
abstract: BACKGROUND
  Mechanical ventilation with high peak inspiratory pressure (PIP) induces lung injury and bacterial translocation from the lung into the systemic circulation. We investigated the effects of increased inspiratory time on translocation of intratracheally inoculated bacteria during mechanical ventilation with and without extrinsic positive end-expiratory pressure (PEEP).
  
  
  METHODS
  Rats were ventilated in pressure-controlled mode with 14 cm H2O PIP, 0 cm H2O PEEP, I:E ratio 1/2, and Fio2 1.0. Subsequently, 0.5 mL of 10(5) cfu/mL Pseudomonas aeruginosa was inoculated through tracheostomy and rats were randomly assigned to six groups; two low-pressure groups (LP)1/2, 14 cm H2O PIP, 0 cm H2O PEEP, I:E = 1/2, and LP2/1 14 cm H2O PIP, 0 cm H2O PEEP, I:E = 2/1; two high-pressure groups (HP)1/2, 30 cm H2O PIP, 0 cm H2O PEEP, I:E = 1/2, and HP2/1, 30 cm H2O PIP, 0 cm H2O PEEP, I:E = 2/1; two HP PEEP groups (HPP)1/2, 30 cm H2O PIP, 10 cm H2O PEEP, I:E = 1/2, and HPP2/1, 30 cm H2O PIP, 10 cm H2O PEEP, I:E = 2/1. Blood cultures were obtained every 30 min. The rats were killed and their lungs were processed.
  
  
  RESULTS
  When compared with baseline values, Pao2 decreased in the LP1/2, LP2/1, HP1/2, and HP2/1 groups at the last time point, but the decline in Pao2 reached statistical significance in only the HP1/2 group. The bacterial translocation rate was greater in group HPP2/1 than group HPP1/2 (P = 0.01).
  
  
  CONCLUSIONS
  We found that high PIP, with or without prolonged inspiratory time, increased the rate of bacterial dissemination. PEEP prevented bacterial translocation in the high PIP group. However, the protective effect of PEEP was lost when inspiratory time was prolonged.
authors.affiliation: Department of Anesthesiology and Intensive Care, Istanbul Medical Faculty, Istanbul University, Capa, Istanbul, Turkey. pergin@istanbul.edu.tr
authors.forename: Perihan Ergin
authors.initials: PE
authors.lastname: Ozcan
authors.forename: Nahit
authors.initials: N
authors.lastname: Cakar
authors.forename: Simru
authors.initials: S
authors.lastname: Tugrul
authors.forename: Ozkan
authors.initials: O
authors.lastname: Akinci
authors.forename: Atahan
authors.initials: A
authors.lastname: Cagatay
authors.forename: Dilek
authors.initials: D
authors.lastname: Yilmazbayhan
authors.forename: Figen
authors.initials: F
authors.lastname: Esen
authors.forename: Lutfi
authors.initials: L
authors.lastname: Telci
authors.forename: Kutay
authors.initials: K
authors.lastname: Akpir
mesh_terms: D000818:Animals; D018988:Bacterial Translocation / Q000502:physiology*; D001239:Inhalation / Q000502:physiology*; D011175:Positive-Pressure Respiration / Q000379:methods; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012121:Respiration, Artificial / Q000379:methods; D013997:Time Factors