title: Changes in the expression of gallinacins, antimicrobial peptides, in ovarian follicles during follicular growth and in response to lipopolysaccharide in laying hens (Gallus domesticus).
abstract: The aim of this study was to identify the types of gallinacin genes (GALs) expressed in ovarian follicles and to determine the changes in their expression during follicular growth and in response to lipopolysaccharide (LPS). Follicles at different stages of growth were collected from laying hens (n = 5) and LPS-injected hens (n = 3). The expression of GALs in the theca and granulosa layers was examined by semi-quantitative RT-PCR. The expression of GAL-1, -2, -7, -8, -10, and -12 in the theca layer and GAL-1, - 8, -10, and -12 in the granulosa layer was identified in white and yellow follicles. The expression of these genes was not changed in the theca and granulosa layers during follicular growth except for a decrease in that of GAL-1 in theca. The expression of GAL-1, -7, and -12 in the theca layer of the third largest follicles was increased in response to LPS at a dose of 1 mg/kg body weight and this increase was induced within 3 h and maintained until 12h postinjection. Granulosa layers did not respond to LPS until 12h injection. These results show that six and four types of GALs are expressed in the theca and granulosa layers of healthy follicles respectively, and their levels do not change with follicular growth except for GAL-1 in theca. Elevated levels of GAL-1, -7, and -12 expression in theca in response to LPS suggest that the theca cells expressing these GALs function to eliminate LPS-containing bacteria.
authors.affiliation: Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan.
authors.forename: Kalpana
authors.initials: K
authors.lastname: Subedi
authors.forename: Naoki
authors.initials: N
authors.lastname: Isobe
authors.forename: Masahide
authors.initials: M
authors.lastname: Nishibori
authors.forename: Yukinori
authors.initials: Y
authors.lastname: Yoshimura
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D002645:Chickens / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D023082:Defensins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D006107:Granulosa Cells / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D006080:Ovarian Follicle / Q000737:chemistry / Q000378:metabolism*; D010058:Oviposition; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013799:Theca Cells / Q000378:metabolism; D023083:beta-Defensins / Q000032:analysis / Q000235:genetics / Q000378:metabolism
chemical_list: D030161:Avian Proteins; D017931:DNA Primers; D023082:Defensins; D008070:Lipopolysaccharides; D023083:beta-Defensins; C086738:gallinacin 2 protein, chicken; C086732:gallinacin 1 protein, chicken