Chemo-enzymatic synthesis and biological evaluation of photolabile nicotinic acid adenine dinuclotide phosphate (NAADP+).
A chemo-enzymatic synthesis of novel caged NAADP+ without the formation of multiple cage compounds has been achieved. The biological activity of the caged NAADP+ was demonstrated by its fast uncaging in intact sea-urchin eggs.
Parkesh
Raman
R
Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK. raman.parkesh@pharm.ox.ac.uk
Vasudevan
Sridhar R
SR
Berry
Alexandra
A
Galione
Antony
A
Dowden
James
J
Churchill
Grant C
GC
D036541:ADP-ribosyl Cyclase / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D005306:Fertilization; D013058:Mass Spectrometry; D009249:NADP / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology; D010063:Ovum / Q000187:drug effects* / Q000378:metabolism; D010777:Photochemistry; D012617:Sea Urchins; D013329:Structure-Activity Relationship
D009249:NADP; C024376:NAADP; D036541:ADP-ribosyl Cyclase; D002118:Calcium