Toxicological stress response and cadmium distribution in hybrid tilapia (Oreochromis sp.) upon cadmium exposure.
Adult tilapia were exposed to 0 (control) and 4.45 microM Cd for 0 h, 5 h, 5 days, and 15 days, and the physiological responses of fish were described. The physiological responses were first expressed in gill tissue, in which mucus cells secretion increased, Cd accumulated, cortisol secretion was significantly higher, but serum ACH(50) activity (alternative complement hemolytic assay) was significantly lower than in controlled fish. After 5 days of Cd exposure, the ACH(50) activity showed a greater decrease, but lysozyme and cortisol contents showed significant increases over the control. Cd levels significantly increased in intestines, liver, and kidneys, and a significant induction of metallothionein (MT) protein in hepatic tissue was noted. Finally, the Cd accumulation rate still showed significant increases in these organs. However, the MT content was similar at 5 days and at 15 days after Cd exposure, and the cortisol contents had recovered to the pre-exposure level. In addition, Cd accumulation in muscle was higher after Cd exposure than in controls (t-test, p<0.05). Our results demonstrate (1) that tilapia readily regulate their physiological parameters in order to acclimate to a sublethal Cd environment; (2) these changes of physiological parameters may be related with a succession of cortisol levels following Cd exposure; (3) increasing rate of hepatic MT contents and Cd accumulation rate didn't appear identical after 5-15 days of Cd exposure. This was confirmed that hepatic MT was not a good indicator for Cd levels in tilapia.
Wu
Su Mei
SM
Department of Aquatic Biosciences, National Chiayi University, Chiayi 600, Taiwan. sumei@mail.ncyu.edu.tw
Shih
Mei-Jyun
MJ
Ho
Yi-Chia
YC
D000818:Animals; D002104:Cadmium / Q000493:pharmacokinetics / Q000633:toxicity*; D002678:Chimera; D003170:Complement Pathway, Alternative / Q000187:drug effects; D005880:Gills / Q000166:cytology / Q000378:metabolism; D006854:Hydrocortisone / Q000097:blood; D007413:Intestinal Mucosa / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008668:Metallothionein / Q000378:metabolism; D009113:Muramidase / Q000097:blood; D009132:Muscles / Q000378:metabolism; D013312:Stress, Physiological / Q000276:immunology / Q000378:metabolism; D017210:Tilapia / Q000276:immunology / Q000378:metabolism
D002104:Cadmium; D008668:Metallothionein; D009113:Muramidase; D006854:Hydrocortisone