[Determination of astragulin in Thesium chinese by RP-HPLC].
OBJECTIVE
To establish a determination method of astragulin in Thesium chinese.


METHODS
RP-HPLC analytical method was established using a Polaris C18 column and acetonitrile-water (23:77) as the mobile phase, with a flow rate of 10 mL x min(-1), detected at 346 nm. The method of sample is refluxing exation by 50% alcohol for 2 times.


RESULTS
The content of astragulin was from 0. 120% to 0. 155%, in different groups of T. chinese collected from the same location.


CONCLUSIONS
The method was validated to show convenient and reliable.
Liu
Yang
Y
School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China.
Deng
Yu-Lin
YL
Yu
Yu-Hong
YH
Dai
Rong-Ji
RJ
Xu
Zhi-Hui
ZH
Meng
Wei-Wei
WW
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005419:Flavonoids / Q000032:analysis*; D005960:Glucosides / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D029630:Santalaceae / Q000737:chemistry*
D005419:Flavonoids; D005960:Glucosides; C524214:astragulin