In vivo evaluation of teicoplanin- and calcium sulfate-loaded PMMA bone cement in preventing implant-related osteomyelitis in rats.
The objective of this study was to evaluate the efficacy of teicoplanin- and calcium sulphate-loaded polymethylmethacrylate (PMMA) bone cements in preventing experimental implant-related osteomyelitis in rats. Four groups of antibiotic-loaded rods were prepared and were implanted into the lateral condylus of the rat femur after inoculation of Staphylococcus aureus. The effectiveness of these were assessed microbiologically, radiographically, and histopathologically. Radiographic evaluation revealed a significant reduction of periostal reaction and osteolysis in rats that received calcium sulphate- and teicoplanin-loaded rods. Histopathological evaluation confirmed these results. Acute infection and bone necrosis were found to be significantly lower in rats that had received calcium sulphate- and teicoplanin-loaded rods. The addition of calcium sulfate to teicoplanin-loaded PMMA bone cement appeared satisfactory as an antibiotic-carrying system for prophylaxis of experimental implant-related osteomyelitis, but further investigations are needed to reach definitive statements for clinical applications.
Tuzuner
T
T
Department of Orthopedics, Abant Izzet Baysal University, Duzce Medical School, Turkey. ttuzuner2003@yahoo.com
Sencan
I
I
Ozdemir
D
D
Alper
M
M
Duman
S
S
Yavuz
T
T
Yildirim
M
M
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000627:therapeutic use; D001843:Bone Cements / Q000737:chemistry / Q000627:therapeutic use*; D002133:Calcium Sulfate / Q000737:chemistry / Q000627:therapeutic use*; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D005260:Female; D005269:Femur / Q000382:microbiology / Q000473:pathology / Q000601:surgery; D010019:Osteomyelitis / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D016459:Prosthesis-Related Infections / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D011514:Proteus vulgaris / Q000187:drug effects / Q000302:isolation & purification; D051381:Rats; D017207:Rats, Sprague-Dawley; D013203:Staphylococcal Infections / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control; D013211:Staphylococcus aureus / Q000187:drug effects / Q000302:isolation & purification; D017334:Teicoplanin / Q000737:chemistry / Q000627:therapeutic use*; D016896:Treatment Outcome
D000900:Anti-Bacterial Agents; D001843:Bone Cements; D017334:Teicoplanin; D019904:Polymethyl Methacrylate; D002133:Calcium Sulfate