Effectiveness of novel PGM-containing incomplete Seppic adjuvants in rabbits.
Peptidoglycan monomer (PGM) is adjuvant active molecule in experimental mice, although its adjuvanticity is much lower in comparison to potent adjuvants. The novel adjuvant formulations were developed by incorporation of PGM into Montanide ISA 206 and Montanide ISA 720 adjuvants, with the aim to enhance its adjuvanticity by protecting it from the fast degradation and metabolic clearance. Adjuvanticity of the novel adjuvant formulations was tested in rabbits for induction of protein-specific antibodies. Both novel adjuvants ISA206(PGM) and ISA720(PGM) were significantly stronger than Montanide adjuvants themselves, and also significantly more potent than Complete Freund Adjuvant. Montanide ISA 720 was shown as much better carrier of PGM, since the novel ISA720(PGM) adjuvant was significantly stronger adjuvant than the ISA206(PGM).
Halassy
Beata
B
Research and Development Department, Institute of Immunology, Inc., Rockefellerova 10, HR-10000 Zagreb, Croatia. bhalassy@imz.hr
Vdović
Valerija
V
Habjanec
Lidija
L
Balija
Maja Lang
ML
Gebauer
Branka
B
Sabioncello
Ante
A
Santek
Tomislav
T
Tomasić
Jelka
J
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D008353:Mannitol / Q000031:analogs & derivatives* / Q000494:pharmacology; D009829:Oleic Acids / Q000494:pharmacology*; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology; D010457:Peptidoglycan / Q000494:pharmacology*; D011817:Rabbits
D000276:Adjuvants, Immunologic; C408982:CPG-oligonucleotide; D007074:Immunoglobulin G; D009829:Oleic Acids; D009838:Oligodeoxyribonucleotides; D010457:Peptidoglycan; C001681:mannide monooleate; D008353:Mannitol; C032972:peptidoglycan pentapeptide-mDap3