title: Reduction of worm fecundity and canine host blood loss mediates protection against hookworm infection elicited by vaccination with recombinant Ac-16.
abstract: Hookworm infection is one of most important parasitic infection of humans, occurring in 740 million people. Here we report the protective vaccination of dogs with Ac-16, an immunodominant surface antigen from the hookworm Ancylostoma caninum. We show that immunization with Ac-16 formulated with AS03 elicited specific humoral and cellular immune responses and provided partial protection against hookworm infection and morbidity as evidenced by a significant reduction of hookworm egg counts (64% reduction; P = 0.0078) and worm-induced blood loss (P < 0.05). Moreover, specific anti-Ac-16 antibodies recognized the native protein on the surface of third-stage larvae and blocked their migration through tissue in vitro. Our data support the use of Ac-16 as a potential candidate for vaccination against hookworm infection.
authors.affiliation: Department of Microbiology, Immunology and Tropical Medicine, The George Washington University, Ross Hall Room 727, 2300 Eye St. NW, Washington, DC 20037, USA.
authors.forename: Ricardo T
authors.initials: RT
authors.lastname: Fujiwara
authors.forename: Bin
authors.initials: B
authors.lastname: Zhan
authors.forename: Susana
authors.initials: S
authors.lastname: Mendez
authors.forename: Alex
authors.initials: A
authors.lastname: Loukas
authors.forename: Lilian L
authors.initials: LL
authors.lastname: Bueno
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Jordan
authors.initials: J
authors.lastname: Plieskatt
authors.forename: Yelena
authors.initials: Y
authors.lastname: Oksov
authors.forename: Sara
authors.initials: S
authors.lastname: Lustigman
authors.forename: Maria Elena
authors.initials: ME
authors.lastname: Bottazzi
authors.forename: Peter
authors.initials: P
authors.lastname: Hotez
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Bethony
mesh_terms: D000595:Amino Acid Sequence; D000722:Ancylostoma / Q000276:immunology*; D000740:Anemia / Q000517:prevention & control*; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D049109:Cell Proliferation; D003001:Cloning, Molecular; D016207:Cytokines / Q000096:biosynthesis; D004285:Dogs; D005260:Female; D015801:Helminth Proteins / Q000235:genetics / Q000276:immunology*; D006725:Hookworm Infections / Q000517:prevention & control*; D008297:Male; D008969:Molecular Sequence Data; D010270:Parasite Egg Count; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D016207:Cytokines; D015801:Helminth Proteins; D014614:Vaccines, Synthetic