Stomatal development and patterning are regulated by environmentally responsive mitogen-activated protein kinases in Arabidopsis.
Stomata are specialized epidermal structures that regulate gas (CO(2) and O(2)) and water vapor exchange between plants and their environment. In Arabidopsis thaliana, stomatal development is preceded by asymmetric cell divisions, and stomatal distribution follows the one-cell spacing rule, reflecting the coordination of cell fate specification. Stomatal development and patterning are regulated by both genetic and environmental signals. Here, we report that Arabidopsis MITOGEN-ACTIVATED PROTEIN KINASE3 (MPK3) and MPK6, two environmentally responsive mitogen-activated protein kinases (MAPKs), and their upstream MAPK kinases, MKK4 and MKK5, are key regulators of stomatal development and patterning. Loss of function of MKK4/MKK5 or MPK3/MPK6 disrupts the coordinated cell fate specification of stomata versus pavement cells, resulting in the formation of clustered stomata. Conversely, activation of MKK4/MKK5-MPK3/MPK6 causes the suppression of asymmetric cell divisions and stomatal cell fate specification, resulting in a lack of stomatal differentiation. We further establish that the MKK4/MKK5-MPK3/MPK6 module is downstream of YODA, a MAPKKK. The establishment of a complete MAPK signaling cascade as a key regulator of stomatal development and patterning advances our understanding of the regulatory mechanisms of intercellular signaling events that coordinate cell fate specification during stomatal development.
Wang
Huachun
H
Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211, USA.
Ngwenyama
Njabulo
N
Liu
Yidong
Y
Walker
John C
JC
Zhang
Shuqun
S
D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D019521:Body Patterning / Q000235:genetics / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D004789:Enzyme Activation; D018506:Gene Expression Regulation, Plant; D005819:Genetic Markers; D020930:MAP Kinase Kinase Kinases / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000502:physiology*; D020929:Mitogen-Activated Protein Kinase Kinases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D009154:Mutation; D018515:Plant Leaves / Q000166:cytology / Q000201:enzymology / Q000254:growth & development
D029681:Arabidopsis Proteins; D005819:Genetic Markers; C458516:AtMPK3 protein, Arabidopsis; C436293:MPK6 protein, Arabidopsis; D020928:Mitogen-Activated Protein Kinases; D020930:MAP Kinase Kinase Kinases; C481302:YODA protein, Arabidopsis; C519871:MKK4 protein, Arabidopsis; C519872:MKK5 protein, Arabidopsis; D020929:Mitogen-Activated Protein Kinase Kinases