[Prognostic and diagnostic value of telomerase in the head and neck cancer].
Telomerase is an enzyme responsible for maintaining the constant length of chromosomal telomers, which are necessary for normal function of eucaryotic cells. Presence of active telomerase in neoplastic cells prevents shortening of telomers what makes this cells immortal. Telomerase plays an important role in carcinogenesis. Aim of this study was to investigate the activity of telomerase in head and neck tumors and assess its diagnostic and prognostic value.


METHODS
Material consisted of 30 head and neck tumors treated surgically and 9 samples from healthy skin and mucous membranes. Telomerase activity was investigated using TeloTAGGG Telomerase PCR ELISA method.


RESULTS
Telomerase activity was found in 24 (80%) malignant tumors. Relative activity of telomerase in neoplasms was from 16.9 to 766,2 RTA. Telomerase expression was much lower in samples of healthy skin and mucous membrane (RTA <5). Statistically significant difference was proven for T1 and T2 tumors, comparing to T4 tumors (p < 0.05). No statistically significant difference in telomerase activity for G1 and G2 differentiation tumors comparing to G3 and for tumors without lymph nodes metastases comparing to tumors with metastases.


CONCLUSIONS
Malignant head and neck tumors show high activity of telomerase comparing to healthy tissue. Detection of telomerase activity in head and neck malignant neoplasms can be a useful marker for cancer assessment. The quantification of telomerase activity has clinical diagnostic value for head and neck malignant neoplasms. To make a convenience of telomerase as a marker for diagnostic and prognosis in patients with malignant head and neck tumor needs further investigations.
Kuczkowski
Jerzy
J
Katedra i Klinika Chorób Uszu, Nosa, Gardła i Krtani AM w Gdańsku. jerzyk@amg.gda.pl
Mikaszewski
Bogusław
B
Pawełczykl
Tadeusz
T
D014408:Biomarkers, Tumor / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000201:enzymology*; D004797:Enzyme-Linked Immunosorbent Assay; D006258:Head and Neck Neoplasms / Q000175:diagnosis* / Q000201:enzymology*; D006801:Humans; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction / Q000379:methods; D011379:Prognosis; D019098:Telomerase / Q000032:analysis*
D014408:Biomarkers, Tumor; D019098:Telomerase