A facile method for construction of antifouling surfaces by self-assembled polymeric monolayers of PEG-silane copolymers formed in aqueous medium.
Self-assembled polymeric monolayers (PMs) on Si/SiO2 wafers were prepared in water from a series of random copolymers of poly(ethylene glycol) methyl ether methacrylate (PEGMA) and 3-(trimethoxysilyl)propyl methacrylate (TMSMA), denoted as poly(TMSMA-r-PEGMA). Four polymers of poly(TMSMA-r-PEGMA) were synthesized by free radical polymerization with a systematic variation of co-monomer feed ratios. Regardless of PEG grafting density in the copolymers, all PMs formed approximately 1 nm-thick film as measured by ellipsometry. However, the PMs with a higher grafting density of PEG resulted in more hydrophilic surfaces in terms of water contact angle. The protein resistance of the PMs was evaluated using bovine serum albumin (BSA) as a model protein. Analyses by ellipsometry, atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS) showed that the PMs of the copolymers markedly reduced the nonspecific adsorption of proteins compared to the unmodified Si/SiO2 wafers. The study also revealed that the PMs prepared from the copolymers with a higher PEG grafting density were more effective in resisting the nonspecific protein adsorption.
Park
Sangjin
S
Department of Life Science, Gwangju Institute of Science and Technology, Gwangiu 500-712, Korea.
Chi
Young Shik
YS
Choi
Insung S
IS
Seong
Jiehyun
J
Jon
Sangyong
S
D000327:Adsorption; D000818:Animals; D002417:Cattle; D004577:Electron Probe Microanalysis; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D008956:Models, Chemical; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012821:Silanes / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D013499:Surface Properties; D014867:Water / Q000737:chemistry
D011108:Polymers; D011506:Proteins; D012821:Silanes; C490327:poly(ethylene glycol)silane; D014867:Water; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D012822:Silicon Dioxide; D012825:Silicon