TMV microarrays: hybridization-based assembly of DNA-programmed viral nanotemplates.
The intrinsic ability of biological molecules to self-organize into complex structures has the potential to revolutionize methods for the assembly of nanomaterials and devices. In this work, nucleic acid hybridization was used to simultaneously assemble different Tobacco mosaic virus (TMV) nanotemplates onto a glass substrate patterned with address specific capture DNAs. To accomplish this, TMV-based nanotemplates were programmed with linker DNAs containing sequence specific addresses and hybridized directly to the capture DNAs. This assembly process proved to be a reliable, selective, and controllable means to assemble multiple TMV nanotemplates.
Yi
Hyunmin
H
Department of Materials Science and Engineering and Institute for Systems Research, University of Maryland, College Park, MD 20742, USA.
Rubloff
Gary W
GW
Culver
James N
JN
D001483:Base Sequence; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000737:chemistry; D005814:Genes, Viral; D016679:Genome, Viral; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D013395:Sucrose / Q000494:pharmacology; D014027:Tobacco Mosaic Virus / Q000235:genetics*
D013395:Sucrose; D004247:DNA