Electro-biosorptive accumulation for use in enhanced detection of fluorogenic tracers and the removal of toxic entities from dilute solutions.
An accumulating process based on electric field-assisted biosorption is described to facilitate the accumulation and enhanced detection of organic fluorogenic marker species in aqueous solution. Fluorescein was detected at concentrations as low as 0.6 microg l(-1). Using a mammalian cell-based bioassay, we demonstrate the use of the system to remove the toxic effects of species such as ethidium bromide and doxorubicin from complex solutions such as tissue culture medium. The use of such a system for the detection and removal of trace contaminants is discussed.
Nomikou
Nikolitsa
N
School of Biomedical Sciences, University of Ulster, Cromore Rd., Coleraine, Co Derry, Northern Ireland, UK.
McHale
Anthony P
AP
D000042:Absorption; D015374:Biosensing Techniques / Q000379:methods*; D045424:Complex Mixtures / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D005456:Fluorescent Dyes / Q000032:analysis*; D008832:Microchemistry / Q000379:methods*; D012996:Solutions; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*
D045424:Complex Mixtures; D005456:Fluorescent Dyes; D012996:Solutions; D014874:Water Pollutants, Chemical; D004247:DNA; C427203:calf thymus DNA