Development of a pair of bifunctional expression vectors for Escherichia coli and Bacillus licheniformis.
A pair of bifunctional expression vectors, pBL-WZX and pHY-WZX, for Escherichia coli and Bacillus licheniformis was constructed to express interesting genes in a secretory manner. The vectors contain an expression cassette consisted of the promoter and signal peptide region of B. licheniformis amyL as well as an artificial multiple cloning site and a terminator and utilize kanamycin-resistance and/or tetracycline-resistance for selection in both B. licheniformis and E. coli. Both vectors contain a part of 3' terminal fragment of B. licheniformis amyL. The 5'-terminal or 3'-terminal fragment of B. licheniformis amyL can cause the integration and amplification of expression cassette in the chromosome of B. licheniformis under a kanamycin-selection pressure. pBL-WZX is an integrational vector while pHY-WZX is free one for B. licheniformis. Both vectors were succeeded in secretory expression of manL in both B. licheniformis and E. coli.
Niu
Dandan
D
The Key Laboratory of Industrial Biotechnology, Ministry of Education and School of Biotechnology, Southern Yangtze University, Wuxi, 214036, China.
Wang
Zheng-Xiang
ZX
D001407:Bacillus / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D018274:Electroporation; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors / Q000737:chemistry*; D007613:Kanamycin Resistance / Q000235:genetics; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000378:metabolism; D013753:Tetracycline Resistance / Q000235:genetics; D014170:Transformation, Genetic; D044902:beta-Mannosidase / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D011994:Recombinant Proteins; D044902:beta-Mannosidase