title: A comparison of the gene expression profiles of CRL-1807 colonocytes exposed to endogenous AAPH-generated peroxides and exogenous peroxides from heated oil.
abstract: Oxidation of PUFAs in the diet has the potential to be genotoxic and hence carcinogenic. Such carcinogenic processes originate within stem cells of the colon. These cells appear to be predisposed to the carcinogenic process. In colon cells (CRL-1807) exposed to chemical reactions simulating exogenous and endogenous peroxidation reactions, we have observed that undifferentiated cells could mount an effective recombinational repair/TCR response to an endogenous peroxidative DNA damage insult, but not to an external exogenous peroxidative insult as one would encounter from a dietary source. This may suggest that defects in such specific DNA repair may play a role in tumour development in undifferentiated colonocytes exposed to a diet-derived lipid peroxides.
authors.affiliation: Molecular Toxicology Group, Department of Forensic Science and Drug Monitoring, School of Health and Life Sciences, King's College London, London, UK. Eugene.Halligan@kcl.ac.uk
authors.forename: Eugene P
authors.initials: EP
authors.lastname: Halligan
authors.forename: Damon
authors.initials: D
authors.lastname: Lowes
authors.forename: Nalini
authors.initials: N
authors.lastname: Mistry
authors.forename: Rosamund
authors.initials: R
authors.lastname: Dove
authors.forename: Marcus
authors.initials: M
authors.lastname: Cooke
authors.forename: Mark
authors.initials: M
authors.lastname: Evans
authors.forename: Joseph
authors.initials: J
authors.lastname: Lunec
mesh_terms: D000578:Amidines / Q000633:toxicity*; D002453:Cell Cycle / Q000187:drug effects; D003106:Colon / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000235:genetics*; D004249:DNA Damage / Q000187:drug effects; D004260:DNA Repair / Q000187:drug effects*; D004032:Diet; D020869:Gene Expression Profiling; D019398:Genes, BRCA1 / Q000187:drug effects; D006358:Hot Temperature; D006801:Humans; D010545:Peroxides / Q000633:toxicity*
chemical_list: D000578:Amidines; D010545:Peroxides; C046728:2,2'-azobis(2-amidinopropane)