Aloe-emodin induces in vitro G2/M arrest and alkaline phosphatase activation in human oral cancer KB cells.
Aloe-emodin is a natural anthraquinone compound from the root and rhizome of Rheum palmatum. In this study, KB cells were treated with 2.5, 5, 10, 20, and 40 microM aloe-emodin for 1 to 5 days. The results showed that aloe-emodin inhibited cancer cells in a dose-dependent manner. Treatment with aloe-emodin at 10 to 40 microM resulted in cell cycle arrest at G2/M phase. The alkaline phosphatase (ALP) activity in KB cells increased upon treatment with aloe-emodin when compared to controls. This is one of the first studies to focus on the expression of ALP in human oral carcinomas cells treated with aloe-emodin. These results indicate that aloe-emodin has anti-cancer effect on oral cancer, which may lead to its use in chemotherapy and chemopreventment of oral cancer.
Xiao
Bingxiu
B
School of Medicine, Ningbo University, Ningbo 315211, China.
Guo
Junming
J
Liu
Donghai
D
Zhang
Shun
S
D000469:Alkaline Phosphatase / Q000378:metabolism; D000880:Anthraquinones / Q000627:therapeutic use*; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D004789:Enzyme Activation; D016195:G2 Phase; D006801:Humans; D007624:KB Cells; D009062:Mouth Neoplasms / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D008517:Phytotherapy
D000880:Anthraquinones; D004365:Drugs, Chinese Herbal; C518327:aloe emodin; D000469:Alkaline Phosphatase