[Expression and clinical significance of autophagy gene Beclin 1 in cervical squamous cell carcinoma].
OBJECTIVE
Autophagy gene Beclin 1 plays an important role in several types of human cancers. So we, in this study, employed the immunohistochemical manner to detect the Beclin 1 gene expression and explore its clinical significance in cervical aquamous cell carcinoma.


METHODS
SP immunohistochemistry technique was used to detect the expression of Beclin 1 gene in specimens of 81 cervical squamous cell carcinoma, 20 cervical intraepithelial neoplasm (CIN, II - III), and 20 normal cervix. Correlations between the expressions of Beclin 1 gene and the clinicopathologic factors of cervical squamous cell carcinoma were statistically analyzed.


RESULTS
The rates of negative, weak, strong expression of Beclin 1 in cervical squamous cell carcinoma were 43.2% (35/81), 34.6% (28/81) or 22.2% (18/81) respectively, and the significantly lower than those in normal cervix and CIN II - III (P = 0.011). The Beclin 1 expressions did not associate with FIGO stage, age, depth of cervical infiltration, tumor size, and gross type of cervical lesion (P < 0.05), but were related to pelvic lymph node metastases and histological tumor grade (P < 0.05).


CONCLUSIONS
s Expression of autophagy gene Beclin 1 decreases in cervical aquamous cell carcinoma, and is closely related to pelvic lymph node metastases and histological tumor grade.
Wang
Zan-Hong
ZH
Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Peng
Zhi-Lan
ZL
Duan
Zhen-Ling
ZL
Liu
Hui
H
D000328:Adult; D000368:Aged; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D001343:Autophagy / Q000235:genetics*; D000071186:Beclin-1; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D016022:Case-Control Studies; D002584:Cervix Uteri / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000378:metabolism*; D008875:Middle Aged; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D002578:Uterine Cervical Dysplasia / Q000235:genetics / Q000378:metabolism / Q000473:pathology
D051017:Apoptosis Regulatory Proteins; C491997:BECN1 protein, human; D000071186:Beclin-1; D008565:Membrane Proteins