Characterization of Campylobacter spp. using restriction fragment length polymorphism and SDS-polyacrylamide gel electrophoresis.
Twenty four Campylobacter jejuni and coli isolates obtained from Egyptian children were characterized using restriction fragment length polymorphism (RFLP) of flagellin genes and polyacrylamide gel electrophoresis of whole cell and glycine-extracted proteins. The isolates were found to fall into nine polymorphism groups, eight of which were reported previously in Egypt but one group displayed by 3 isolates represented a new group that was not reported before. Furthermore, the relative prevalence of polymorphic groups in the population studied is different from that reported previously. Analysis of whole-cell and acid glycine-extracted proteins showed that the profiles of these isolates are typical profiles of Campylobacters isolated from other humans.
Hadi
H A
HA
Enteric Microbiology Branch, U.S. Naval Medical Research Unit No. 3, Cairo, Egypt.
Mohran
Z S
ZS
Hakam
A A
AA
Mourad
A
A
Oyofo
B A
BA
D017000:Campylobacter coli / Q000235:genetics* / Q000302:isolation & purification; D016123:Campylobacter jejuni / Q000235:genetics* / Q000302:isolation & purification; D002648:Child; D004534:Egypt; D004591:Electrophoresis, Polyacrylamide Gel; D005408:Flagellin / Q000235:genetics; D005798:Genes, Bacterial; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length
D005408:Flagellin; C067138:flaA protein, bacteria