Development of a LC/MS/MS method for the analysis of cannabinoids in human EDTA-plasma and urine after small doses of Cannabis sativa extracts.
A novel high-performance liquid chromatographic separation method with tandem-mass spectrometry detection was developed for the simultaneous determination of Delta(9)-tetrahydrocannabinol (THC) and its major metabolites 11-hydroxy-Delta(9)-tetrahydrocannabinol (11-OH-THC) and 11-nor-Delta(9)-tetrahydrocannabinol-9-carboxylic acid (THC-COOH) as well as the components cannabidiol (CBD) and cannabinol (CBN) in human EDTA-plasma and urine. Run time was 25 min. Lower limit of quantification was 0.2 ng/ml. The coefficients of variation of all inter- and intra-assay determinations were between 1.3 and 15.5%. The method was successfully applied to the determination of cannabinoids in human plasma and human urine after administration of Delta(9)-tetrahydrocannabinol or Cannabis sativa extracts.
Grauwiler
Sandra B
SB
Clinical Chemical Laboratory, Toxicology Section, University Hospital, Petersgraben 4, 4031 Basel, Switzerland.
Scholer
André
A
Drewe
Jürgen
J
D002186:Cannabinoids / Q000097:blood* / Q000493:pharmacokinetics / Q000652:urine*; D002188:Cannabis / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D004492:Edetic Acid / Q000737:chemistry*; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
D002186:Cannabinoids; D010936:Plant Extracts; D004492:Edetic Acid