title: Altered HDAC5 and CREB mRNA expressions in the peripheral leukocytes of major depression.
abstract: BACKGROUND
  Gene expressions of the peripheral leukocytes in depressive patients might reflect the systemic dysfunction of major depression. We determined mRNA expression levels of Histone deacetylase 5 (HDAC5) gene and cyclic AMP response element-binding protein 1 (CREB) gene in the leukocyte of depressive patients. HDAC5 and CREB are reported to be important targets of antidepressants, the latter being located in the downstream of the former in lymphocyte calcium signaling.
  
  
  METHODS
  25 patients with major depression and 25 age- and sex-matched healthy controls were included in this study. Twenty patients were able to be followed up until the 8 week-treatment. The mRNA levels were determined by a quantitative RT-PCR method.
  
  
  RESULTS
  Levels of HDAC5 and CREB mRNA were significantly higher in drug-free depressive patients than those of controls and the higher mRNA levels decreased to control levels after 8-week paroxetine treatment. There were positive correlation between levels of HDAC5 and CREB.
  
  
  CONCLUSIONS
  Our results suggest the alteration of HDAC5 and CREB gene expression in the systemic pathophysiology of major depression.
authors.affiliation: Department of Psychiatry, Course of Integrated Brain Sciences, University of Tokushima School of Medicine, Tokushima 770-8503, Japan.
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Iga
authors.forename: Shu-Ichi
authors.initials: S
authors.lastname: Ueno
authors.forename: Ken
authors.initials: K
authors.lastname: Yamauchi
authors.forename: Shusuke
authors.initials: S
authors.lastname: Numata
authors.forename: Sawako
authors.initials: S
authors.lastname: Kinouchi
authors.forename: Sumiko
authors.initials: S
authors.lastname: Tayoshi-Shibuya
authors.forename: Hongwei
authors.initials: H
authors.lastname: Song
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Ohmori
mesh_terms: D000328:Adult; D000368:Aged; D050882:CREB-Binding Protein / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D003865:Major Depressive Disorder / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D017374:Paroxetine / Q000097:blood; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018709:Statistics, Nonparametric
chemical_list: D012333:RNA, Messenger; D017374:Paroxetine; D050882:CREB-Binding Protein; C494363:CREBBP protein, human; C404500:HDAC5 protein, human; D006655:Histone Deacetylases