[Sampling of exhaled gas after intravenous administration of octafluoropropane-containing human albumin micropheres in dogs].
OBJECTIVE
To develop a method for quantitative collection of exhaled gas in anesthetized dogs at given time following intravenous administration of octafluoropropane (OFP)-containing human albumin micropheres for assessing the gas kinetics of OFP.


METHODS
OFP-containing albumin micropheres were administered intravenously at 0.4, 0.8 and 1.2 ml/kg, respectively, in anesthetized and ventilated dogs. The exhaled air samples were analyzed by gas chromatography-tandem mass spectrometry (GC-MS-MS).


RESULTS
The correlation curve between the area under curve (AUC) and administered dose was roughly linear (Y=1162.5X-417.38, r square=0.949 9). The total recovery rate was (119.49-/+27.62)% which was not significantly different from the rate of 100% (P>0.05). GC-MS-MS was accurate, sensitive, precise and applicable for OFP determination.


CONCLUSIONS
The sampling method is useful for characterizing OFP pharmacokinetics in dogs, and also applicable for studying the pharmacokinetics of other gas-containing drugs.
Yang
Ling
L
Department of Pharmacy, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Chen
Zhi-liang
ZL
Liu
Shi-ting
ST
Yang
Li
L
D000418:Albumins / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics*; D000818:Animals; D004285:Dogs; D045853:Exhalation; D005260:Female; D005466:Fluorocarbons / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D007275:Injections, Intravenous; D008297:Male; D008863:Microspheres; D015203:Reproducibility of Results
D000418:Albumins; D005466:Fluorocarbons; C042852:perflutren