Chemically induced expression of rice OSB2 under the control of the OsPR1.1 promoter confers increased anthocyanin accumulation in transgenic rice.
Anthocyanin pigmentation provides an excellent system with which to study the regulation of gene expression in higher plants. In this study, OsPR1.1 promoter was isolated and the promoter activity was monitored using a reporter gene OSB2, which encodes a transcription factor for anthocyanin synthesis in rice plants. We introduced PR::OSB2 plasmid into an isogenic Taichung 65, no. 99-962 T-65 CBA B9F5 (T65 CBA), rice line (Oryza sativa L.) and found that the transgenic rice plants exhibited anthocyanin accumulation by the induced expression of OSB2 after chemical treatments with methyl jasmonate (MeJA) and 2,6-dichloroisonicotinic acid (DCINA). The shoots of the PR::OSB2 transgenic rice plants changed color to red after application of the chemicals accompanying with the increased anthocyanin content to approximately 5-fold by MeJA and 2-fold by DCINA, respectively. The anthocyanin accumulation was consistent with the increase of the expression of OSB2 and anthocyanidin synthase (ANS). This color change system could provide a useful and easy way to produce transgenic plants for monitoring of chemicals in the environment.
Kawahigashi
Hiroyuki
H
National Institute of Agrobiological Sciences (NIAS), 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan. shiwak@affrc.go.jp
Hirose
Sakiko
S
Iwai
Takayoshi
T
Ohashi
Yuko
Y
Sakamoto
Wataru
W
Maekawa
Masahiko
M
Ohkawa
Yasunobu
Y
D000085:Acetates / Q000494:pharmacology; D000872:Anthocyanins / Q000096:biosynthesis* / Q000235:genetics; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics*; D003517:Cyclopentanes / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects*; D007539:Isonicotinic Acids / Q000494:pharmacology; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D054883:Oxylipins; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics*
D000085:Acetates; D000872:Anthocyanins; D051792:Basic Helix-Loop-Helix Proteins; D003517:Cyclopentanes; D007539:Isonicotinic Acids; D054883:Oxylipins; D010940:Plant Proteins; C443663:PLW-OSB2 protein, Oryza sativa; C077261:2,6-dichloroisonicotinic acid; C072239:methyl jasmonate