title: Distinguishing between cis/trans isomers of monounsaturated fatty acids by FAB MS.
abstract: Fast-atom bombardment (FAB) mass spectrometry in the negative ion mode can be used to unambiguously distinguish between cis and trans isomers of monounsaturated fatty acids by the relative signal strengths of an intense pair of ion signals. Under normal FAB ionization/desorption conditions, the deprotonated molecules (i.e., [M - H]-) of six fatty acids underwent charge remote fragmentation. A characteristic fragmentation pattern of two intense peak clusters of peaks with three weak intervening clusters of peaks are used in each case to identify the position of the double bond. The possibility of resonance electron capture occurring during the FAB process is discussed.
authors.affiliation: Department of Chemistry, Oregon State University, Corvallis, Oregon 97331, USA.
authors.forename: Hong
authors.initials: H
authors.lastname: Ji
authors.forename: Valery G
authors.initials: VG
authors.lastname: Voinov
authors.forename: Max L
authors.initials: ML
authors.lastname: Deinzer
authors.forename: Douglas F
authors.initials: DF
authors.lastname: Barofsky
mesh_terms: D005229:Fatty Acids, Monounsaturated / Q000032:analysis*; D012680:Sensitivity and Specificity; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000379:methods*; D013237:Stereoisomerism
chemical_list: D005229:Fatty Acids, Monounsaturated