title: Nitrosative stress in plants.
abstract: Nitrosative stress has become a usual term in the physiology of nitric oxide in mammalian systems. However, in plants there is much less information on this type of stress. Using olive leaves as experimental model, the effect of salinity on the potential induction of nitrosative stress was studied. The enzymatic l-arginine-dependent production of nitric oxide (NOS activity) was measured by ozone chemiluminiscence. The specific activity of NOS in olive leaves was 0.280nmol NOmg(-1) proteinmin(-1), and was dependent on l-arginine, NADPH and calcium. Salt stress (200mM NaCl) caused an increase of the l-arginine-dependent production of nitric oxide (NO), total S-nitrosothiols (RSNO) and number of proteins that underwent tyrosine nitration. Confocal laser scanning microscopy analysis using either specific fluorescent probes for NO and RSNO or antibodies to S-nitrosoglutathione and 3-nitrotyrosine, showed also a general increase of these reactive nitrogen species (RNS) mainly in the vascular tissue. Taken together, these findings show that in olive leaves salinity induces nitrosative stress, and vascular tissues could play an important role in the redistribution of NO-derived molecules during nitrosative stress.
authors.affiliation: Grupo de Señalización Molecular y Sistemas Antioxidantes en Plantas, Unidad Asociada al CSIC (EEZ), Area de Bioquímica y Biología Molecular, Universidad de Jaén, E-23071 Jaén, Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Valderrama
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Corpas
authors.forename: Alfonso
authors.initials: A
authors.lastname: Carreras
authors.forename: Ana
authors.initials: A
authors.lastname: Fernández-Ocaña
authors.forename: Mounira
authors.initials: M
authors.lastname: Chaki
authors.forename: Francisco
authors.initials: F
authors.lastname: Luque
authors.forename: María V
authors.initials: MV
authors.lastname: Gómez-Rodríguez
authors.forename: Pilar
authors.initials: P
authors.lastname: Colmenero-Varea
authors.forename: Luis A
authors.initials: LA
authors.lastname: Del Río
authors.forename: Juan B
authors.initials: JB
authors.lastname: Barroso
mesh_terms: D018613:Microscopy, Confocal; D009569:Nitric Oxide / Q000378:metabolism*; D031658:Olea / Q000187:drug effects / Q000378:metabolism; D009994:Osmolar Concentration; D010944:Plants / Q000378:metabolism*; D026361:Reactive Nitrogen Species / Q000378:metabolism; D026422:S-Nitrosoglutathione / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism
chemical_list: D026361:Reactive Nitrogen Species; D013481:Superoxides; D009569:Nitric Oxide; D012965:Sodium Chloride; D026422:S-Nitrosoglutathione