title: Comparison of quantity and structures of hydroxyproline-containing peptides in human blood after oral ingestion of gelatin hydrolysates from different sources.
abstract: We compared quantity and structures of food-derived gelatin hydrolysates in human blood from three sources of type I collagen in a single blind crossover study. Five healthy male volunteers ingested type I gelatin hydrolysates from fish scale, fish skin, or porcine skin after 12 h of fasting. Amounts of free form Hyp and Hyp-containing peptide were measured over a 24-h period. Hyp-containing peptides comprised approximately 30% of all detected Hyp. The total area under the concentration-time curve of the fish scale group was significantly higher than that of the porcine skin group. Pro-Hyp was a major constituent of Hyp-containing peptides. Ala-Hyp, Leu-Hyp, Ile-Hyp, Phe-Hyp, and Pro-Hyp-Gly were detected only with fish scale or fish skin gelatin hydrolysates. Ala-Hyp-Gly and Ser-Hyp-Gly were detected only with fish scale gelatin hydrolysate. The quantity and structure of Hyp-containing peptides in human blood after oral administration of gelatin hydrolysate depends on the gelatin source.
authors.affiliation: Food and Health R&D Laboratories, Meiji Seika Kaisha, Ltd., 5-3-1, Chiyoda, Sakado-shi, Saitama 350-0289, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Ohara
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Matsumoto
authors.forename: Kyoko
authors.initials: K
authors.lastname: Ito
authors.forename: Koji
authors.initials: K
authors.lastname: Iwai
authors.forename: Kenji
authors.initials: K
authors.lastname: Sato
mesh_terms: D000328:Adult; D000818:Animals; D024042:Collagen Type I / Q000008:administration & dosage / Q000737:chemistry; D018592:Cross-Over Studies; D005399:Fishes; D005780:Gelatin / Q000008:administration & dosage*; D006801:Humans; D006909:Hydroxyproline / Q000097:blood*; D008297:Male; D010455:Peptides / Q000097:blood*; D011492:Protein Hydrolysates / Q000008:administration & dosage*; D012867:Skin / Q000737:chemistry; D013552:Swine
chemical_list: D024042:Collagen Type I; D010455:Peptides; D011492:Protein Hydrolysates; D005780:Gelatin; D006909:Hydroxyproline