title: A novel alternative spliced variant of neutrophil gelatinase-associated lipocalin receptor in oesophageal carcinoma cells.
abstract: Recent studies suggest that NGAL (neutrophil gelatinase-associated lipocalin) is a novel iron transporter with functions distinct from that of transferrin and mediates a new iron-delivery pathway. To get a better understanding of NGAL function in oesophageal carcinoma, we analysed the expression of NGAL receptors in oesophageal carcinoma cells and identified a novel spliced variant designated NgalR-3. When expressed in a heterologous system, the protein produced from this novel spliced variant exhibits the biochemical characteristics of interaction and co-localization with NGAL protein in vivo. This new finding suggests that NgalR-3 may act as a potential NGAL receptor and play a role in NGAL-mediated iron transport in oesophageal carcinoma.
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College of Shantou University, Shantou 515041, Guangdong Province, China.
authors.forename: Wang-Kai
authors.initials: WK
authors.lastname: Fang
authors.forename: Li-Yan
authors.initials: LY
authors.lastname: Xu
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Lu
authors.forename: Lian-Di
authors.initials: LD
authors.lastname: Liao
authors.forename: Wei-Jia
authors.initials: WJ
authors.lastname: Cai
authors.forename: Zhong-Ying
authors.initials: ZY
authors.lastname: Shen
authors.forename: En-Min
authors.initials: EM
authors.lastname: Li
mesh_terms: D000209:Acute-Phase Proteins / Q000235:genetics* / Q000378:metabolism*; D017398:Alternative Splicing / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics; D004938:Esophageal Neoplasms / Q000201:enzymology* / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D014644:Genetic Variation / Q000235:genetics*; D006801:Humans; D000071068:Lipocalin-2; D054834:Lipocalins; D008969:Molecular Sequence Data; D027701:Organic Cation Transport Proteins; D011485:Protein Binding; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D018000:Receptors, Peptide / Q000235:genetics* / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: D000209:Acute-Phase Proteins; D018076:DNA, Complementary; C489506:LCN2 protein, human; D000071068:Lipocalin-2; D054834:Lipocalins; D027701:Organic Cation Transport Proteins; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D018000:Receptors, Peptide; C518841:SLC22A17 protein, human