Establishment and characterization of a cell line derived from a human serous surface papillary carcinoma of the ovary.
A novel serous surface papillary carcinoma of the ovary (SSPC) cell line, HYKSSPC, was established successfully. Carcinoma cells were obtained from ascitic fluid of a 60-year-old Japanese woman. The population doubling time was 51.4 h. A phase contrast micrograph showed a pavement stone-like arrangement without contact inhibition. The chromosome number showed a wide distribution of aneuploidy, and the mode was in 46-47. An immunocytochemical study showed that CA125, BerER4 and cytokeratin were positive and that CEA, calretinin and thrombomodulin were negative. This cell line preserved some characters of the adenocarcinoma while growing in vitro. A chemosensitivity test revealed that HYKSSPC cells were sensitive to CDDP (cis-platinum), 5-fluorouracil, mitomycin C, paclitaxel and irinotecan. To our knowledge, HYKSSPC is the first established cell line derived from SSPC, and it may offer some useful information for investigating this disease.
Kobayashi
Yoichi
Y
Department of Obstetrics and Gynecology, St Marianna University, School of Medicine, Kawasaki, and Ishiwata Hospital, Mito, Japan. y5koba@marianna-u.ac.jp
Mizuhara
Hiroshi
H
Ohara
Tatsuru
T
Kondo
Haruhiro
H
Sato
Sojiro
S
Kiguchi
Kazushige
K
Ishizuka
Bunpei
B
Ishiwata
Isamu
I
Tadokoro
Mamoru
M
D000782:Aneuploidy; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D018394:CA-125 Antigen / Q000032:analysis; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division; D045744:Cell Line, Tumor; D018283:Cystadenocarcinoma, Papillary / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D019008:Drug Resistance, Neoplasm; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D014183:Transplantation, Heterologous
D000970:Antineoplastic Agents; D018394:CA-125 Antigen