title: Electrochemical detection in a microfluidic device of oxidative stress generated by macrophage cells.
abstract: The release of reactive oxygen species (ROS) or reactive nitrogen species (RNS), i.e., the initial phase of oxidative stress, by macrophage cells has been studied by electrochemistry within a microfluidic device. Macrophages were first cultured into a detection chamber containing the three electrodes system and were subsequently stimulated by the microinjection of a calcium ionophore (A23187). Their production of ROS and RNS was then measured by amperometry at the surface of a platinized microelectrode. The fabricated microfluidic device provides an accurate measurement of oxidative release kinetics with an excellent reproducibility. We believe that such a method is simple and versatile for a number of advanced applications based on the detection of biological processes of secretion by a few or even a single living cell.
authors.affiliation: Ecole Normale Supérieure, Département de Chimie, UMR CNRS-ENS-UPMC 8640 "PASTEUR", 24 rue Lhomond, Paris Cedex 05, 75231, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Amatore
authors.forename: Stéphane
authors.initials: S
authors.lastname: Arbault
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.forename: Cécile
authors.initials: C
authors.lastname: Crozatier
authors.forename: Issa
authors.initials: I
authors.lastname: Tapsoba
mesh_terms: D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002138:Calibration; D002460:Cell Line; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D005609:Free Radicals; D007476:Ionophores / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D018384:Oxidative Stress; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D013997:Time Factors
chemical_list: D005609:Free Radicals; D007476:Ionophores; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D000001:Calcimycin