title: Sealer penetration and marginal permeability after apicoectomy varying retrocavity preparation and retrofilling material.
abstract: Apicoectomy failure is generally related to inappropriate marginal sealing of the retrocavity, which allows percolation of microorganisms and their products from root canal system to periapex. This study evaluated tubular penetration of canal sealers and marginal permeability after retrocavity irradiation with Er;Cr:YSGG laser and retrofilling with MTA or cyanoacrylate. Twenty-two single-rooted teeth were decoronated and endodontically treated, their apical 3 mm were resected and the root ends were retroprepared with a low-speed bur. Twenty roots were randomly assigned to 4 groups (n=5): GI and GII--retrofilling with MTA and cyanoacrylate, respectively; GIII and GIV--retrocavity irradiation with Er;Cr:YSGG laser (2.78 microm, 4 W, 20 Hz, 70.8 J/cm(2)) and retrofilling with MTA and cyanoacrylate, respectively. The remaining 2 roots served as positive and negative controls. The analysis of rhodamine B dye infiltration (p=0.05) demonstrated that laser irradiation and MTA retrofilling presented significantly higher permeability rates (p<0.05). Retrofilling with cyanoacrylate showed significantly lower permeability, either when laser was used or not on retrocavity. SEM analysis depicted more cyanoacrylate penetration through dentinal tubules when compared to MTA, suggesting a more efficient marginal sealing. Based on these results, it may be concluded that cyanoacrylate provided a less permeable retrofilling regardless of the retropreparation method, suggesting a more favorable condition to the establishment of the periapical healing.
authors.affiliation: Special Laboratory of Lasers in Dentistry, Center of Research, Teaching and Clinics of Laser in Dentistry.
authors.forename: Renata
authors.initials: R
authors.lastname: Winik
authors.forename: Angela Toshie
authors.initials: AT
authors.lastname: Araki
authors.forename: José Augusto Alves
authors.initials: JA
authors.lastname: Negrão
authors.forename: Marina Stella
authors.initials: MS
authors.lastname: Bello-Silva
authors.forename: José Luiz
authors.initials: JL
authors.lastname: Lage-Marques
mesh_terms: D017607:Aluminum Compounds / Q000737:chemistry / Q000627:therapeutic use; D001047:Apicoectomy; D017610:Calcium Compounds / Q000737:chemistry / Q000627:therapeutic use; D003487:Cyanoacrylates / Q000737:chemistry / Q000627:therapeutic use; D001840:Dental Bonding; D003763:Dental Leakage / Q000145:classification; D003786:Dental Pulp Cavity / Q000648:ultrastructure; D003804:Dentin / Q000648:ultrastructure; D004338:Drug Combinations; D005456:Fluorescent Dyes; D006801:Humans; D053685:Laser Therapy; D008855:Microscopy, Electron, Scanning; D010087:Oxides / Q000737:chemistry / Q000627:therapeutic use; D010539:Permeability; D012184:Retrograde Obturation / Q000379:methods*; D012235:Rhodamines; D012387:Root Canal Filling Materials / Q000737:chemistry / Q000627:therapeutic use*; D018915:Root Canal Preparation / Q000379:methods*; D017640:Silicates / Q000737:chemistry / Q000627:therapeutic use; D013499:Surface Properties
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D003487:Cyanoacrylates; D004338:Drug Combinations; D005456:Fluorescent Dyes; D010087:Oxides; D012235:Rhodamines; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; C029773:rhodamine B