title: Antimicrobial analysis of different root canal filling pastes used in pediatric dentistry by two experimental methods.
abstract: The objective of this study was to compare, by two experimental methods, the antimicrobial efficacy of different root canal filling pastes used in pediatric dentistry. The tested materials were: Guedes-Pinto paste (GPP), zinc oxide-eugenol paste (OZEP), calcium hydroxide paste (CHP), chloramphenicol + tetracycline + zinc oxide and eugenol paste (CTZP) and Vitapex. Fiven microbial strains (S. aureus, E. faecalis, P. aeruginosa, B. subtilis and C. albicans) obtained from the American Type Culture Collection were inoculated in Brain Heart Infusion (BHI) and incubated at 37 degrees C for 24 h. For the direct exposure test (DET), 72 paper points were contaminated with the standard microbial suspensions and exposed to the root canal filling pastes for 1, 24, 48 and 72 h. The points were immersed in Letheen Broth (LB), followed by incubation at 37 degrees C for 48 h. An inoculum of 0.1 mL obtained from LB was then transferred to 7 mL of BHI, under identical incubations conditions and the microbial growth was evaluated. The pastes showed activity between 1 and 24 h, depending on the material. For the agar diffusion test (ADT), 30 Petri plates with 20 mL of BHI agar were inoculated with 0.1 mL of the microbial suspension, using sterile swabs that were spread on the medium. Three cavities were made in each agar plate (total = 90) and completely filled with one of the filling root canal pastes. The plates were pre-incubated for 1 h at room temperature and then incubated at 37 degrees C for 24 to 48 h. The inhibition zone around each well was recorded in mm. The complete antimicrobial effect in the direct exposure test was observed after 24 h on all microbial indicators. All root canal filling materials induced the formation of inhibition zones, except for Vitapex (range, 6.0-39.0 mm).
authors.affiliation: School of Dentistry, Federal University of Goiás, Rua 1, 74115-040 Goiânia, GO, Brazil. lilianguedes@cultura.com.br
authors.forename: Lilian de Fátima Guedes de
authors.initials: Lde F
authors.lastname: Amorim
authors.forename: Orlando Airton de
authors.initials: OA
authors.lastname: Toledo
authors.forename: Cyntia Rodrigues de Araújo
authors.initials: CR
authors.lastname: Estrela
authors.forename: Daniel de Almeida
authors.initials: Dde A
authors.lastname: Decurcio
authors.forename: Carlos
authors.initials: C
authors.lastname: Estrela
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects; D002126:Calcium Hydroxide / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects; D002701:Chloramphenicol / Q000494:pharmacology; D015169:Colony Count, Microbial; D003786:Dental Pulp Cavity / Q000382:microbiology*; D004338:Drug Combinations; D013293:Enterococcus faecalis / Q000187:drug effects; D006801:Humans; D006847:Hydrocarbons, Iodinated / Q000494:pharmacology; D010377:Pediatric Dentistry; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D012828:Silicones / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects; D013752:Tetracycline / Q000494:pharmacology; D013997:Time Factors; D015035:Zinc Oxide-Eugenol Cement / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D000891:Anti-Infective Agents, Local; D004338:Drug Combinations; D006847:Hydrocarbons, Iodinated; D012387:Root Canal Filling Materials; D012828:Silicones; D015035:Zinc Oxide-Eugenol Cement; C029584:calcium hydroxide, iodoform, silicone oil drug combination; D002701:Chloramphenicol; D013752:Tetracycline; C010473:iodoform; D002126:Calcium Hydroxide