[Immune response to HSP70-HBcAg(18-27) complex in HBV transgenic mice].
OBJECTIVE
To study the cellular immune response to HSP70-HBcAg(18-27) complex in HBV transgenic mice.


METHODS
HSP70-HBcAg(18-27) complex was reconstituted in vitro, then it was injected into HBV transgenic mice to observe the cellular immune response. At the same time, we investigated whether HSP70-HBcAg(18-27) complex could generate antigen specific cytotoxic T lymphocyte responses in spleen cells.


RESULTS
Our results demonstrated that HSP70-HBcAg(18-27) complex increased levels of CD4+ and CD8+ T cells in the spleens and peripheral blood of HBV transgenic mice, and the complex also activated dendritic and natural killer cells.


CONCLUSIONS
HSP70-HBcAg(18-27) complex has an immunological antigenicity in raising the immunoresponse to chronic HBV infection in HBV transgenic mice. HSP70-HBcAg(18-27) complex might be considered as a candidate for further studies on its role as a therapeutic vaccine against chronic HBV infection in humans.
Hu
Huai-dong
HD
Key Laboratory of Molecular Biology for Infectious Diseases, PRC Ministry of Education, Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China.
Hu
Peng
P
Chen
Guo-lin
GL
Peng
Ming-li
ML
Chen
Min
M
Ren
Hong
H
D000818:Animals; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology*; D006512:Hepatitis B Core Antigens / Q000276:immunology*; D006515:Hepatitis B virus / Q000235:genetics / Q000276:immunology*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D013154:Spleen / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D018840:HSP70 Heat-Shock Proteins; D006512:Hepatitis B Core Antigens