title: Smaragdicoccus niigatensis gen. nov., sp. nov., a novel member of the suborder Corynebacterineae.
abstract: A polyphasic taxonomic approach was applied to determine the taxonomic position of a hydrocarbon-degrading actinomycete, strain Hou_blueT, which was isolated from soil samples collected from an oil spring in Niigata, Japan. The results of 16S rRNA and gyrB gene sequence comparisons indicated that strain Hou_blueT represented a novel lineage in the suborder Corynebacterineae. Colonies were malachite green-like in colour on 1/10 trypticase soy agar and the cell morphology was coccoid in all growth phases. The cell-wall diamino acid and sugar indicated chemotype IV and variation A1gamma. The sugars of the peptidoglycan were glycolated. The polar lipids were composed of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and some unspecified glycolipids. The organism contained two novel cyclic forms of menaquinone, smaragdiquinone A-8(H4, omega-cycl) and smaragdiquinone B-8(H4, dicycl). The major fatty acids were cis-9-18 : 1 (34.46 %) and 16 : 0 (25.1 %). Small amounts of 10-methyl-branched fatty acids were also present (10-methyl-17 : 0, 0.17 %), but not tuberculostearic acid (10-methyl-18 : 0), which has been shown to be present in all nocardiae. Gas-chromatographic analysis of the mycolic acid revealed a carbon-chain length of C43-C49. The DNA G+C content was 63.7 mol%. On the basis of phenotypic and phylogenetic distinctness, the organism is proposed to represent a novel genus and species, Smaragdicoccus niigatensis gen. nov., sp. nov., with the type strain Hou_blueT (=MBIC 06267T=DSM 44881T).
authors.affiliation: Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, Iwate 026-0001, Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Adachi
authors.affiliation: Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, Iwate 026-0001, Japan.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Katsuta
authors.affiliation: Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, Iwate 026-0001, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Matsuda
authors.affiliation: Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, Iwate 026-0001, Japan.
authors.forename: Xue
authors.initials: X
authors.lastname: Peng
authors.affiliation: Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, Iwate 026-0001, Japan.
authors.forename: Norihiko
authors.initials: N
authors.lastname: Misawa
authors.affiliation: Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, Iwate 026-0001, Japan.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Shizuri
authors.affiliation: DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkultren GmbH, Inhoffenstrasse 7b, D-38124 Braunschweig, Germany.
authors.forename: Reiner M
authors.initials: RM
authors.lastname: Kroppenstedt
authors.affiliation: Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Yokota
authors.affiliation: Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, Iwate 026-0001, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kasai
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D000599:Amino Acids, Diamino / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D002473:Cell Wall / Q000737:chemistry; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry; D020459:Genes, rRNA; D006838:Hydrocarbons / Q000378:metabolism; D007564:Japan; D008969:Molecular Sequence Data; D009171:Mycolic Acids / Q000032:analysis; D010457:Peptidoglycan / Q000737:chemistry; D010578:Petroleum / Q000382:microbiology*; D010743:Phospholipids / Q000032:analysis / Q000737:chemistry; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D011809:Quinones / Q000032:analysis / Q000737:chemistry; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D000599:Amino Acids, Diamino; D002241:Carbohydrates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D006838:Hydrocarbons; D009171:Mycolic Acids; D010457:Peptidoglycan; D010578:Petroleum; D010743:Phospholipids; D010860:Pigments, Biological; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D027081:DNA Gyrase