title: High-resolution mapping for essential hypertension using microsatellite markers.
abstract: During the past decade, considerable efforts and resources have been devoted to elucidating the multiple genetic and environmental determinants responsible for hypertension and its associated cardiovascular diseases. The success of positional cloning, fine mapping, and linkage analysis based on whole-genome screening, however, has been limited in identifying multiple genetic determinants affecting diseases, suggesting that new research strategies for genome-wide typing may be helpful. Disease association (case-control) studies using microsatellite markers, distributed every 150 kb across the human genome, may have some advantages over linkage, candidate, and single nucleotide polymorphism typing methods in terms of statistical power and linkage disequilibrium for finding genomic regions harboring candidate disease genes, although it is not proven. We have carried out genome-wide mapping using 18,977 microsatellite markers in a Japanese population composed of 385 hypertensive patients and 385 normotensive control subjects. Pooled sample analysis was conducted in a 3-stage genomic screen of 3 independent case-control populations, and 54 markers were extracted from the original 18,977 microsatellite markers. As a final step, each single positive marker was confirmed by individual typing, and only 19 markers passed this test. We identified 19 allelic loci that were significantly different between the cases of essential hypertension and the controls.
authors.affiliation: Department of Medical Science and Cardiorenal Medicine, Yokohama City University School of Medicine, Yokohama, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Yatsu
authors.forename: Nobuhisa
authors.initials: N
authors.lastname: Mizuki
authors.forename: Nobuhito
authors.initials: N
authors.lastname: Hirawa
authors.forename: Akira
authors.initials: A
authors.lastname: Oka
authors.forename: Norihiko
authors.initials: N
authors.lastname: Itoh
authors.forename: Takahiro
authors.initials: T
authors.lastname: Yamane
authors.forename: Momoko
authors.initials: M
authors.lastname: Ogawa
authors.forename: Tadashi
authors.initials: T
authors.lastname: Shiwa
authors.forename: Yasuharu
authors.initials: Y
authors.lastname: Tabara
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Ohno
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Soma
authors.forename: Akira
authors.initials: A
authors.lastname: Hata
authors.forename: Kazuwa
authors.initials: K
authors.lastname: Nakao
authors.forename: Hirotsugu
authors.initials: H
authors.lastname: Ueshima
authors.forename: Toshio
authors.initials: T
authors.lastname: Ogihara
authors.forename: Hitonobu
authors.initials: H
authors.lastname: Tomoike
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Miki
authors.forename: Akinori
authors.initials: A
authors.lastname: Kimura
authors.forename: Shuhei
authors.initials: S
authors.lastname: Mano
authors.forename: Jerzy K
authors.initials: JK
authors.lastname: Kulski
authors.forename: Satoshi
authors.initials: S
authors.lastname: Umemura
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Inoko
mesh_terms: D000328:Adult; D000483:Alleles; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D006973:Hypertension / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D008875:Middle Aged