Detection of tumor markers using single-walled carbon nanotube field effect transistors.
We have developed a biosensor capable of detecting carcinoembryonic antigen (CEA) markers using single-walled carbon nanotube field effect transistors (SWNT-FETs). These SWNT-FETs were fabricated using nanotubes produced by a patterned catalyst growth technique, where the top contact electrodes were generated using conventional photolithography. For biosensor applications, SU-8 negative photoresist patterns were used as an insulation layer. CEA antibodies were employed as recognition elements to specific tumor markers, and were successfully immobilized on the sides of a single-walled carbon nanotube using CDI-Tween 20 linking molecules. The binding of tumor markers to these antibody-functionalized SWNT-FETs was then monitored continuously during exposure to dilute CEA solutions. The observed sharp decrease in conductance demonstrates the possibility of realizing highly sensitive, label-free SWNT-FET-based tumor sensors.
Park
Dong-Won
DW
Department of Chemical Engineering, Chungbuk National University, Cheongju 500-712, Korea.
Kim
Yo-Han
YH
Kim
Beom Soo
BS
So
Hye-Mi
HM
Won
Keehoon
K
Lee
Jeong-O
JO
Kong
Ki-Jeong
KJ
Chang
Hyunju
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015374:Biosensing Techniques; D002272:Carcinoembryonic Antigen / Q000096:biosynthesis*; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D006801:Humans; D051379:Mice; D036103:Nanotechnology / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009369:Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D011136:Polysorbates / Q000737:chemistry; D012680:Sensitivity and Specificity; D013997:Time Factors
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D037742:Nanotubes, Carbon; D011136:Polysorbates