Biological imaging of HEK293 cells expressing PLCgamma1 using surface-enhanced Raman microscopy.
Surface-enhanced Raman scattering (SERS) imaging has been used for the targeting and imaging of specific cancer markers in live cells. For this purpose, Au/Ag core-shell nanoparticles, conjugated with monoclonal antibodies, were prepared. The procedures to label live cells with those bimetallic nanoprobes have been developed and used for highly sensitive SERS imaging of live cells. In the present study, live HEK293 cells expressing PLCgamma1 have been used as the optical imaging target. Our results demonstrate the potential feasibility of SERS imaging technology for the highly sensitive imaging of cancer biomarkers in live cells.
Lee
Sangyeop
S
Department of Applied Chemistry, Hanyang University, Ansan 426-791, South Korea.
Kim
Sungyong
S
Choo
Jaebum
J
Shin
Soon Young
SY
Lee
Young Han
YH
Choi
Ha Young
HY
Ha
Seunghan
S
Kang
Kyungho
K
Oh
Chil Hwan
CH
D000911:Antibodies, Monoclonal; D002460:Cell Line; D003584:Cytological Techniques; D003952:Diagnostic Imaging / Q000379:methods*; D006046:Gold; D006801:Humans; D053768:Metal Nanoparticles; D009369:Neoplasms / Q000473:pathology*; D051966:Phospholipase C gamma / Q000032:analysis*; D013059:Spectrum Analysis, Raman / Q000379:methods*
D000911:Antibodies, Monoclonal; D006046:Gold; D051966:Phospholipase C gamma