Resistin-binding peptide antagonizes role of resistin on white adipose tissue.
OBJECTIVE
To investigate the direct effects of resistin and resistin-binding peptide (RBP) on lipid metabolism and endocrine function in adipose tissue.


METHODS
Rat white adipose tissue was cultured in vitro and incubated for 24 h with 30 ng/mL recombinant rat resistin protein (rResistin) or combined with RBP of varying concentrations(1x10(-12) mol/L, 1x10(-10) mol/L, 1x10(-8) mol/L). Free fatty acids (FFA) released into medium was measured by a colorimetric kit. The levels of protein secretion and mRNA expression of TNF-alpha and adiponectin were detected by ELISA kit and RT-PCR respectively.


RESULTS
The levels of FFA released into medium were significantly increased after 24 h of exposure to rResistin, but significantly decreased after RBP was applied, although there was no difference between the 3 concentrations. The protein level and gene expression of TNF-alpha in adipose tissue were significantly increased after 24 h of exposure to rResistin, but only obviously decreased after incubated with 1x10(-8) mol/L RBP. The levels of protein secretion and mRNA expression of adiponectin in adipose tissue were significantly decreased after 24 h of exposure to rResistin, but increased after incubated with RBP with the higher concentrations.


CONCLUSIONS
RBP can effectively antagonize the role of resistin on the lipid metabolism and endocrine function of adipose tissue.
Gu
Nan
N
Department of Pediatrics, Nanjing Maternal and Child Health Hospital of Nanjing Medical University, Nanjing 210029, China.
Han
Shu-ping
SP
Fei
Li
L
Pan
Xiao-qin
XQ
Guo
Mei
M
Chen
Rong-hua
RH
Guo
Xi-rong
XR
D052242:Adiponectin / Q000235:genetics; D052436:Adipose Tissue, White / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins / Q000502:physiology*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D008066:Lipolysis; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D052243:Resistin / Q000037:antagonists & inhibitors / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D052242:Adiponectin; D002352:Carrier Proteins; D005230:Fatty Acids, Nonesterified; D052243:Resistin; C499622:Retn protein, rat; D014409:Tumor Necrosis Factor-alpha