title: Further evidence of the role of frizzled-related protein gene polymorphisms in osteoarthritis.
abstract: OBJECTIVE
  To replicate the association of frizzled-related protein (FRZB) non-synonymous polymorphisms with osteoarthritis (OA) susceptibility.
  
  
  METHODS
  Three groups of Spanish patients with OA were included: with total joint replacement due to primary OA in the hip (n = 310), or the knee (n = 277), or with hand OA (n = 242). Controls were more than 55 years old and did not show OA (n = 294). SNPs rs288326 (R200W) and rs7775 (R324G) were genotyped.
  
  
  RESULTS
  There were no significant differences in allele frequencies between controls and each of the three groups of OA patients. However, allele G of the R324G SNP showed a trend to be more frequent in patients with a clinical OA syndrome at multiple joints (p = 0.07), specifically in women of the total hip replacement group (8.3% in patients without other affected joints, 13.1% with one, 15.9% with two and 24.1% with more than two additional joints, p for trend = 0.008).
  
  
  CONCLUSIONS
  No direct replication of previous OA association findings was obtained but the results suggest that the R324G SNP of the FRZB gene may have an effect in OA development in multiple joints, with a specific severe involvement of the hip in women. This phenotype could reconcile previous studies that showed association either with generalised OA or with hip OA in women.
authors.affiliation: Laboratorio de Investigacion 2, Hospital Clinico Universitario de Santiago, Travesia de Choupana sn, 15706-Santiago de Compostela, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Rodriguez-Lopez
authors.forename: M
authors.initials: M
authors.lastname: Pombo-Suarez
authors.forename: M
authors.initials: M
authors.lastname: Liz
authors.forename: J J
authors.initials: JJ
authors.lastname: Gomez-Reino
authors.forename: A
authors.initials: A
authors.lastname: Gonzalez
mesh_terms: D000368:Aged; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006023:Glycoproteins / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D010003:Osteoarthritis / Q000235:genetics*; D015207:Osteoarthritis, Hip / Q000235:genetics; D020370:Osteoarthritis, Knee / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D000099274:Frizzled-Related Proteins
chemical_list: D006023:Glycoproteins; D047908:Intracellular Signaling Peptides and Proteins; D000099274:Frizzled-Related Proteins