Rapid quantification of polycyclic aromatic hydrocarbons in hydroxypropyl-beta-cyclodextrin (HPCD) soil extracts by synchronous fluorescence spectroscopy (SFS).
Synchronous fluorescence spectroscopy (SFS) was directly applied to rapidly quantify selected polycyclic aromatic hydrocarbons (PAHs: benzo[a]pyrene and pyrene) in aqueous hydroxypropyl-beta-cyclodextrin (HPCD) soil extract solutions from a variety of aged contaminated soils containing four different PAHs. The method was optimized and validated. The results show that SFS can be used to analyse benzo[a]pyrene and pyrene in HPCD based soil extracts with high sensitivity and selectivity. The linear calibration ranges were 4.0x10(-6)-1.0x10(-3)mM for benzo[a]pyrene and 6.0x10(-6)-1.2x10(-3)mM for pyrene in 10mM HPCD aqueous solution alone. The detection limits according to the error propagation theory for benzo[a]pyrene and pyrene were 3.9x10(-6) and 5.4x10(-6)mM, respectively. A good agreement between SFS and HPLC was reached for both determinations of PAHs in HPCD alone and in soil HPCD extracts. Hence, SFS is a potential means to simplify the present non-exhaustive hydroxypropyl-beta-cyclodextrin (HPCD)-based extraction technique for the evaluation of PAH bioavailability in soil.
Hua
Guoxiong
G
School of Biology and Psychology, Institute for Research on Environment and Sustainability, Newcastle University, Newcastle upon Tyne, UK. gh15@st-andrews.ac.uk
Broderick
John
J
Semple
Kirk T
KT
Killham
Ken
K
Singleton
Ian
I
D000073738:2-Hydroxypropyl-beta-cyclodextrin; D001564:Benzo(a)pyrene / Q000032:analysis; D004784:Environmental Monitoring / Q000379:methods*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis*; D011721:Pyrenes / Q000032:analysis; D012989:Soil Pollutants / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods; D047392:beta-Cyclodextrins
D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; D012989:Soil Pollutants; D047392:beta-Cyclodextrins; D000073738:2-Hydroxypropyl-beta-cyclodextrin; D001564:Benzo(a)pyrene; C030984:pyrene