Constitutive IL-10 expression by lung inflammatory cells and risk for bronchopulmonary dysplasia.
Expression of IL-10 is decreased in lungs of preterm infants. We determined the constitutive and lipopolysaccharide (LPS)-induced IL-10 synthesis by lung inflammatory cells from preterm and term infants and examined their relationship to gestational age and/or incidence of bronchopulmonary dysplasia (BPD). A total of 37 infants; preterm neonates at gestational ages of 23-27 wk (group 1); 28-34 wk (group 2), and four full-term infants with meconium aspiration (group 3) were enrolled. One sample of lung inflammatory cells, obtained during postnatal d 1-3, and another during postnatal d 4-7 were cultured in vitro in presence or absence of 100 mug/mL of LPS. Secreted IL-10 was measured by ELISA. A positive relationship was found between gestational age and LPS-induced, but not constitutive IL-10 production within 1-3 d of life; group 1 on d 1-3 had a significant number of IL-10 nonresponders compared with group 2. All term neonates in group 3 had positive LPS-induced IL-10 response. Thus, in utero maturation of IL-10 gene expression is due to acquisition of inducibility. In contrast, constitutive IL-10 production within d 1-3 of life correlated with, and predicted the incidence of BPD in the highly vulnerable very premature infants.
Garingo
Arlene
A
Division of Neonatology, Department of Pediatrics, Women's & Children's Hospital, Keck School of Medicine, University of Southern California, Los Angeles, California, 90033, USA.
Tesoriero
Linda
L
Cayabyab
Rowena
R
Durand
Manuel
M
Blahnik
Martin
M
Sardesai
Smeeta
S
Ramanathan
Rangasamy
R
Jones
Craig
C
Kwong
Kenny
K
Li
Changgong
C
Minoo
Parviz
P
D001997:Bronchopulmonary Dysplasia / Q000453:epidemiology*; D002478:Cells, Cultured; D005260:Female; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000235:genetics; D016209:Interleukin-8 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008168:Lung / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D008297:Male; D011014:Pneumonia / Q000276:immunology*; D011446:Prospective Studies; D012307:Risk Factors
D016209:Interleukin-8; D008070:Lipopolysaccharides; D016753:Interleukin-10