Rapid detection of Panton-Valentine leukocidin-positive Staphylococcus aureus by real-time PCR targeting the lukS-PV gene.
In order to assess the speed and accuracy of a real-time PCR assay targeting the lukS-PV gene of Panton-Valentine leukocidin (PVL)-positive Staphylococcus aureus, 700 S. aureus strains were tested and the results were compared to those achieved with block cycler PCR. Cross-reactivity was tested with 166 other bacterial species. Using this homogeneous real-time PCR assay format, the presence or absence of genetic information for PVL, which is also found in community-associated methicillin-resistant S. aureus, was correctly identified from pure culture and directly in various types of clinical specimens.
Reischl
U
U
Institute of Medical Microbiology and Hygiene, University Hospital of Regensburg, Franz-Josef-Strauss-Allee 11, 93053, Regensburg, Germany. udo.reischl@klinik.uni-regensburg.de
Tuohy
M J
MJ
Hall
G S
GS
Procop
G W
GW
Lehn
N
N
Linde
H
H
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001427:Bacterial Toxins / Q000235:genetics* / Q000378:metabolism; D003470:Culture Media; D005098:Exotoxins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007956:Leukocidins / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D013997:Time Factors
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003470:Culture Media; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; C108483:leukocidin S-component protein, Staphylococcus