title: Structural characterization of microcystins by LC/MS/MS under ion trap conditions.
abstract: LC/MS/MS under ion trap conditions was used to analyze microcystins produced by cyanobacteria. Tandem mass spectrometry using MS2 was quite effective since ions arising from cleavage at a peptide bond provide useful sequence information. The fragmentation was confirmed by a shifting technique using structurally-related microcystins and the resulting fragmentation pattern was different from those determined by triple stage MS/MS and four sector MS/MS. Analysis of a mixture of microcystins in a bloom sample was successfully performed and two new microcystins were identified by LC/MS/MS under ion trap conditions. Thus, LC/MS/MS under ion trap conditions is effective for the structural characterization of microcystins.
authors.affiliation: Graduate School of Environmental and Human Science & Faculty of Pharmacy, Meijo University, Tempaku, Nagoya 468-8503, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Mayumi
authors.forename: Hajime
authors.initials: H
authors.lastname: Kato
authors.forename: Susumu
authors.initials: S
authors.lastname: Imanishi
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Kawasaki
authors.forename: Masateru
authors.initials: M
authors.lastname: Hasegawa
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Harada
mesh_terms: D000595:Amino Acid Sequence; D002853:Chromatography, Liquid / Q000379:methods; D000458:Cyanobacteria / Q000737:chemistry*; D052998:Microcystins / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D052998:Microcystins