title: Flavobacterium defluvii sp. nov., isolated from activated sludge.
abstract: A Gram-negative bacterium, designated strain EMB117T, was isolated from a municipal wastewater treatment plant and characterized by polyphasic taxonomy. The cells were non-spore-forming rods that showed gliding motility. Optimal growth occurred at 25-30 degrees C and pH 7.0-8.0. Strain EMB117T contained phosphatidylethanolamine as the predominant polar lipid, and the major fatty acids were iso-C(15 : 0), iso-C(17 : 0) 3-OH, iso-C(15 : 0) 3-OH and summed feature 3 (C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH). The G+C content of the genomic DNA was 33.5 mol% and the major isoprenoid quinone was MK-6. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain EMB117T belonged to the genus Flavobacterium and was most closely related to Flavobacterium johnsoniae DSM 425(T) (97.8 % sequence similarity). The DNA-DNA relatedness between strain EMB117T and F. johnsoniae ATCC 17061T was about 18 %. On the basis of the phenotypic, chemotaxonomic and molecular data, strain EMB117T represents a novel species within the genus Flavobacterium, for which the name Flavobacterium defluvii sp. nov. is proposed. The type strain is EMB117T (=KCTC 12612T=DSM 17963T).
authors.affiliation: Division of Applied Life Science, EB-NCRC, PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Minjeong
authors.initials: M
authors.lastname: Park
authors.affiliation: Division of Applied Life Science, EB-NCRC, PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Seung Hyun
authors.initials: SH
authors.lastname: Ryu
authors.affiliation: Division of Applied Life Science, EB-NCRC, PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Thu-Huong Thi
authors.initials: TT
authors.lastname: Vu
authors.affiliation: Department of Microbiology and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Hyeon-Su
authors.initials: HS
authors.lastname: Ro
authors.affiliation: Jeju Hi-Tech Industry Development Institute, 4-8 Ara-1 dong, Jeju 690-121, Republic of Korea.
authors.forename: Pil-Yong
authors.initials: PY
authors.lastname: Yun
authors.affiliation: Division of Applied Life Science, EB-NCRC, PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Che Ok
authors.initials: CO
authors.lastname: Jeon
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry; D005417:Flavobacterium / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D020459:Genes, rRNA / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D007723:Korea; D008969:Molecular Sequence Data; D009068:Movement; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000032:analysis / Q000302:isolation & purification; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D013696:Temperature; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012722:Sewage; C483858:phosphatidylethanolamine