title: [Investigation of role in antitumor immune of mouse recombinant alpha-fetoprotein conjugated with mannan].
abstract: OBJECTIVE
  To investigate how to deliver AFP to the immune system for generating the appropriate immune response.
  
  
  METHODS
  We prepared the oxidized mannan linked to recombinant mouse alpha-fetoprotein (mAFP) as a vaccine (Ox-M-mAFP), and the vaccine based on mAFP without mannan modification was also prepared as a control.
  
  
  RESULTS
  We found that immunotherapy with Ox-M-mAFP was effective on protective and therapeutic antitumor immunity in mice. No marked toxicity was found in the immunized mice.
  
  
  CONCLUSIONS
  These findings may be important to further exploration of the breaking of immune tolerance to self molecules, and may provide a new vaccine strategy for the treatment of AFP-positive hepatocellular carcinoma.
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Yu-Quan
authors.initials: YQ
authors.lastname: Wei
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Chen
authors.forename: Ji-Yan
authors.initials: JY
authors.lastname: Liu
authors.forename: Yang
authors.initials: Y
authors.lastname: Wu
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Ding
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Wen
authors.forename: Jin-Liang
authors.initials: JL
authors.lastname: Yang
authors.forename: Ling-Yu
authors.initials: LY
authors.lastname: Fan
authors.forename: Jiong
authors.initials: J
authors.lastname: Li
authors.forename: Bing
authors.initials: B
authors.lastname: Kan
authors.forename: Hong-Xin
authors.initials: HX
authors.lastname: Deng
mesh_terms: D000818:Animals; D019496:Cancer Vaccines / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D045744:Cell Line, Tumor; D005260:Female; D008214:Lymphocytes / Q000276:immunology; D008351:Mannans / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009369:Neoplasms / Q000276:immunology*; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism*; D000509:alpha-Fetoproteins / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D019496:Cancer Vaccines; D008351:Mannans; D011994:Recombinant Proteins; D000509:alpha-Fetoproteins