Genetic Mapping of the pho2, PHO82-pho4 and pho85 Loci of Yeast.
The PHO82-pho4 (PHOO-phoD) locus was mapped on the right arm of chromosome VI, 6.5 cM centromere-distal to met10. A pho85 (phoU) mutant was newly isolated, and its map location was determined on the left arm of chromosome XVI, 15 cM centromere-distal to rad1. A mutant gene that causes temperature-sensitive growth on nutrient medium was found very near (or at) the pho85 locus. The supertriploid method was successfully applied to locate the pho2 (phoB) locus on chromosome IV. The pho2 locus was 40 cM from rna11 on the left arm of chromosome IV.
Toh-E
A
A
Laboratory of Biochemical Pharmacology, National Institute of Arthritis, Metabolism, and Digestive Diseases, National Institutes of Health, Bethesda, Maryland 20205.