title: Glucose improves the in vitro viability of Microsporum canis and Trichophyton mentagrophytes var. mentagrophytes.
abstract: We describe simple and cost-effective methods using carbohydrates to improve the in vitro viability of dermatophytes. Glucose and sucrose in different concentrations (3, 6, 9 and 12%) were used to maintain fifteen strains of M. canis and T. mentagrophytes var. mentagrophytes at 4 and -20 degrees C. The strains were phenotypically analyzed before storage and reevaluated at 1, 3, 6 and 9 months. At 1 and 3 months, any alterations in the viability or phenotype pattern of the stored strains were noted. At 6 months, both dermatophytes were 100% viable, when preserved in glucose (3, 6, 9 and 12%) at -20 degrees C. All T. mentagrophytes strains were also viable in sucrose (12%), at 4 degrees C and -20 degrees C. However, sucrose failed to improve the viability of M. canis at both temperatures. At 9 months, the higher viabilities without pleomorphism were seen for both dermatophytes preserved in glucose (9 and 12%) at -20 degrees C.
authors.affiliation: Veterinary Faculty, Post-Graduation Program in Veterinary Science, State University of Ceará, Fortaleza, CE, Brazil.
authors.forename: F A Soares
authors.initials: FA
authors.lastname: Júnior
authors.forename: R S N
authors.initials: RS
authors.lastname: Brilhante
authors.forename: R A
authors.initials: RA
authors.lastname: Cordeiro
authors.forename: E H S
authors.initials: EH
authors.lastname: Brito
authors.forename: J J C
authors.initials: JJ
authors.lastname: Sidrim
authors.forename: M F G
authors.initials: MF
authors.lastname: Rocha
mesh_terms: D003883:Arthrodermataceae / Q000166:cytology / Q000187:drug effects; D018929:Cell Culture Techniques; D005947:Glucose / Q000494:pharmacology*; D008864:Microsporum / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D013395:Sucrose / Q000494:pharmacology; D014249:Trichophyton / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure
chemical_list: D013395:Sucrose; D005947:Glucose