title: Hydrophilic interaction chromatography using methacrylate-based monolithic capillary column for the separation of polar analytes.
abstract: A porous zwitterionic monolith was prepared by thermal copolymerization of N,N-dimethyl-N-methacryloxyethyl-N-(3-sulfopropyl)ammonium betaine and ethylene dimethacrylate inside a 100-mum-i.d. capillary. The resulting monolith was evaluated as a hydrophilic liquid chromatography (HILIC) stationary phase. No evidence of swelling or shrinking of the monolith in different polarity solvents was observed. A typical HILIC mechanism was observed at higher organic solvent content (ACN% > 60%). The poly(SPE-co-EDMA) monolith showed very good selectivity for neutral, basic, and acidic polar analytes. For charged analytes, both hydrophilic interactions and electrostatic interactions contributed to their retention, which provide chromatographers more choice to optimize the separations.
authors.affiliation: Pharmaceutical Sciences Research Division, King's College London, London, SE1 9NH, UK.
authors.forename: Zhengjin
authors.initials: Z
authors.lastname: Jiang
authors.forename: Norman W
authors.initials: NW
authors.lastname: Smith
authors.forename: Paul D
authors.initials: PD
authors.lastname: Ferguson
authors.forename: Mark R
authors.initials: MR
authors.lastname: Taylor
mesh_terms: D053801:Capillary Electrochromatography / Q000379:methods*; D002845:Chromatography; D008689:Methacrylates; D009930:Organic Chemicals / Q000302:isolation & purification; D010539:Permeability; D055672:Static Electricity
chemical_list: D008689:Methacrylates; D009930:Organic Chemicals