title: Molecular bases of viral RNA targeting by viral small interfering RNA-programmed RISC.
abstract: RNA silencing is conserved in a broad range of eukaryotes and operates in the development and maintenance of genome integrity in many organisms. Plants have adapted this system for antiviral defense, and plant viruses have in turn developed mechanisms to suppress RNA silencing. RNA silencing-related RNA inactivation is likely based on target RNA cleavage or translational arrest. Although it is widely assumed that virus-induced gene silencing (VIGS) promotes the endonucleolytic cleavage of the viral RNA genome, this popular assumption has never been tested experimentally. Here we analyzed the viral RNA targeting by VIGS in tombusvirus-infected plants, and we show evidence that antiviral response of VIGS is based on viral RNA cleavage by RNA-induced silencing effector complex (RISC) programmed by virus-specific small interfering RNAs (siRNAs). In addition, we found that the RISC-mediated cleavages do not occur randomly on the viral genome. Indeed, sequence analysis of cloned cleavage products identified hot spots for target RNA cleavage, and the regions of specific RISC-mediated cleavages are asymmetrically distributed along the positive- and negative-sense viral RNA strands. In addition, we identified viral siRNAs containing high-molecular-mass protein complexes purified from the recovery leaves of the silencing suppressor mutant virus-infected plants. Strikingly, these large nucleoproteins cofractionated with microRNA-containing complexes, suggesting that these nucleoproteins are silencing related effector complexes.
authors.affiliation: Agricultural Biotechnology Center, P.O. Box 411, H-2101 Gödöllo, Hungary.
authors.forename: Vitantonio
authors.initials: V
authors.lastname: Pantaleo
authors.forename: György
authors.initials: G
authors.lastname: Szittya
authors.forename: József
authors.initials: J
authors.lastname: Burgyán
mesh_terms: D002458:Cell Fractionation; D046911:Macromolecular Substances / Q000302:isolation & purification; D035683:MicroRNAs / Q000302:isolation & purification; D009698:Nucleoproteins / Q000302:isolation & purification / Q000378:metabolism; D018515:Plant Leaves / Q000821:virology; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism*; D034743:RNA-Induced Silencing Complex / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics* / Q000821:virology*; D017864:Tombusvirus / Q000235:genetics / Q000502:physiology*
chemical_list: D046911:Macromolecular Substances; D035683:MicroRNAs; D009698:Nucleoproteins; D034741:RNA, Small Interfering; D012367:RNA, Viral; D034743:RNA-Induced Silencing Complex