A single component ratiometric pH probe with long wavelength excitation of europium emission.
Following excitation in the range 370-405 nm, the emission spectrum of a cell permeable macrocyclic Eu(III) complex incorporating an N-methylsulfonamide moiety changes form with pH, allowing ratiometric pH measurements in the range 6 to 8.
Pal
Robert
R
Department of Chemistry, Durham University, South Road, Durham, UKDH1 3LE.
Parker
David
D
D000818:Animals; D005063:Europium / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D008163:Luminescent Measurements; D047028:Macrocyclic Compounds / Q000737:chemistry*; D051379:Mice; D041681:NIH 3T3 Cells; D011874:Radiometry
D005456:Fluorescent Dyes; D008024:Ligands; D047028:Macrocyclic Compounds; D005063:Europium