title: Multiresidue analysis of seven anticoagulant rodenticides by high-performance liquid chromatography/electrospray/mass spectrometry.
abstract: Mice and rat populations are commonly controlled by two classes of rodenticide anticoagulants, coumarins and indandiones. However, poisoning of nontarget animals also often occurs. For cases such as these, a rapid, multiresidue method, which provides positive confirmation for both classes of anticoagulant rodenticides, is needed by diagnostic laboratories. A method was developed for the determination of seven anticoagulant rodenticides, coumafuryl, pindone, warfarin, diphacinone, chlorophacinone, bromadiolone, and brodifacoum, in diverse matrices, animal feed, cooked beef, and fruit-flavored beverages using high-performance liquid chromatography/electrospray/mass spectrometry. Detection was by MS/MS with electrospray ionization in negative mode. Confirmation was by retention time, m/z of molecular ion, and two parent-daughter transitions. Recoveries from selected the matrices ranged from 61 to 117%. Limits of quantitation were as low as 1.5-4.5 ng g-1. The developed method was rapid and provided the simultaneous confirmation and quantification of the seven anticoagulant rodenticides.
authors.affiliation: Minnesota Department of Agriculture, 601 Robert Street North, St. Paul, MN 55155, USA. LeEtta.Marek@state.mn.us
authors.forename: LeEtta J
authors.initials: LJ
authors.lastname: Marek
authors.forename: William C
authors.initials: WC
authors.lastname: Koskinen
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D000925:Anticoagulants / Q000032:analysis*; D001628:Beverages / Q000032:analysis; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003374:Coumarins / Q000032:analysis; D005506:Food Contamination / Q000032:analysis; D007189:Indans / Q000032:analysis; D008460:Meat / Q000032:analysis; D010573:Pesticide Residues / Q000032:analysis*; D012378:Rodenticides / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: C413384:1,2-indanedione; D000925:Anticoagulants; D003374:Coumarins; D007189:Indans; D010573:Pesticide Residues; D012378:Rodenticides