A uniform HPLC method developed for the analysis of Salvia miltiorrhiza, Panax notoginseng, and Fufang Danshen.
Fufang Danshen (FFDS) is a famous typical Chinese complex prescription, which is mainly composed of Radix Salvia miltiorrhiza Bunge (SM) and Radix Panax notoginseng (PN). An HPLC method is developed to analyze SM, PN, and FFDS effectively; the effective analysis is achieved by using a gradient elution procedure with a mobile phase consisting of acetonitrile and 0.025% aqueous phosphoric acid (v/v). Through this method, 33 peaks in FFDS are clearly exhibited, and the components that make up the 33 peaks in FFDS are evaluated. Also, the chemical ingredients are compared between the single herbs (SM and PN) and the complex prescription (FFDS). The result indicate that the chemical ingredients in FFDS are not simply a combination of SM and PN. In addition, the HPLC method is suitable for the routine quality control of SM, PN, and FFDS, which could present a uniform quality control method for single medicines and one of the most commonly used Traditional Chinese Medicine-complex prescriptions.
Zeng
Guifeng
G
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Liu
Jianxun
J
Wang
Longxing
L
Xu
Qing
Q
Xiao
Hongbin
H
Liang
Xinmiao
X
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D053723:Panax notoginseng / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D027561:Salvia miltiorrhiza / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; C517866:fufang danshen