A novel method to fabricate patterned bilayer lipid membranes.
We introduce a new method for forming tethered bilayer lipid membranes on surfaces patterned using a photocleavable self-assembled monolayer (SAM). A SAM terminated with a hydrophobic fluorocarbon residue was bound to a gold surface through a link containing a photocleavable ortho-nitrobenzyl moiety. Hydrophilic regions were produced by irradiation with soft UV (365 nm) through a photomask. The patterned surface was characterized by scanning electron microscopy and electrochemical impedance spectroscopy. Tethered bilayer lipid membranes with well-defined bilayer and monolayer regions were then formed by exposure to egg PC vesicles. The membranes had resistance and capacitance values of 0.52 MOmega.cm2 and 0.83 microF.cm-2, respectively.
Han
Xiaojun
X
School of Physics and Astronomy, and Self-Organising Molecular Systems (SOMS) Centre, University of Leeds, LS2 9JT Leeds, United Kingdom.
Critchley
Kevin
K
Zhang
Lixin
L
Pradeep
Singh N D
SN
Bushby
Richard J
RJ
Evans
Stephen D
SD
D003432:Cross-Linking Reagents; D004563:Electrochemistry; D006046:Gold; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry; D008722:Methods; D008855:Microscopy, Electron, Scanning; D010713:Phosphatidylcholines; D013057:Spectrum Analysis; D013499:Surface Properties
D003432:Cross-Linking Reagents; D008051:Lipid Bilayers; D008081:Liposomes; D010713:Phosphatidylcholines; D006046:Gold