Purification and characterization of new special ginsenosidase hydrolyzing multi-glycisides of protopanaxadiol ginsenosides, ginsenosidase type I.
In this paper, the new type ginsenosidase which hydrolyzing multi-glycosides of ginsenoside, named ginsenoside type I from Aspergillus sp.g48p strain was isolated, characterized and generally described. The enzyme molecular weight was about 80 kDa. Ginsenosidase type I can hydrolyze different glycoside of protopanaxadiol type ginsenosides (PPD); i.e., can hydrolyze the 3(carbon)-O-beta-glucoside of Rb1, Rb2, Rb3, Rc, Rd; can hydrolyze 20(carbon)-O-beta-glucoside of Rb1, 20-O-beta-xyloside of Rb3, 20-O-alpha-arabinoside(p) of Rb2 and 20-O-alpha-arabinoside(f) of Rc to produce mainly F2, compound-K (C-K) and small Rh2, but can not hydrolyze the glycosides of protopanaxatriol type ginsenoside (PPT) such as Re, Rf, Rg1. So, when the ginsenosidase type I hydrolyzed ginsenosides, the enzyme selected ginsenoside-aglycone type, can hydrolyze different glycosides of PPD type ginsenoside; however no selected glycoside type, can hydrolyze multi-glycosides of PPD type ginsenosides. These properties were novel properties, and differentiated with the other previously described glycosidases.
Yu
Hongshan
H
College of Bio & Food Technology, Dalian Institute of Light Industry Qinggong-yuan No 1, Dalian, People's Republic of China.
Zhang
Chunzhi
C
Lu
Mingchun
M
Sun
Fang
F
Fu
Yaoyao
Y
Jin
Fengxie
F
D001232:Aspergillus fumigatus / Q000201:enzymology*; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D036145:Ginsenosides / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008970:Molecular Weight; D010946:Plants, Medicinal; D012502:Sapogenins / Q000378:metabolism*; D013696:Temperature; D014315:Triterpenes / Q000378:metabolism*
D036145:Ginsenosides; D006026:Glycoside Hydrolases; D012502:Sapogenins; D014315:Triterpenes; C112772:ginsenoside compound K; C518847:ginsenosidase type I, Aspergillus; C062916:protopanaxadiol