title: Relationship between the presence of the citrate permease plasmid and high electron-donor surface properties of Lactococcus lactis ssp. lactis biovar. diacetylactis.
abstract: Some strains of Lactococcus lactis subspecies possess a citrate permease that enables them to utilize citrate and to produce diacetyl. Such strains are classified as diacetylactis biovariants (L. lactis ssp. lactis biovar. diacetylactis). We investigated the electron-donor surface properties of L. lactis strains and observed that the diacetylactis biovariants presented increased adhesion to electron-acceptor solvents (microbial adhesion to solvents electron-donor characteristics of cells of <27% for L. lactis and about 50% for L. lactis ssp. lactis biovar diacetylactis). We investigated the properties of a pCitP- derivative and observed for a diacetylactis biovariant strain a loss of the electron-donor characteristics falling from 47% for a pCitP+ strain to 8% for its pCitP- derivative. This suggests that the presence of high electron-donor characteristics on the surface of L. lactis results to a large extent from the presence of the citrate permease plasmid.
authors.affiliation: Laboratoire de Microbiologie UMR UB/INRA 1232, Qualités des Aliments, ENSBANA, Dijon, France.
authors.forename: Mai Hu'o'ng
authors.initials: MH
authors.lastname: Lý
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Cavin
authors.forename: Rémy
authors.initials: R
authors.lastname: Cachon
authors.forename: Thanh Mai
authors.initials: TM
authors.lastname: Lê
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Belin
authors.forename: Yves
authors.initials: Y
authors.lastname: Waché
mesh_terms: D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics*; D002462:Cell Membrane / Q000502:physiology; D003931:Diacetyl / Q000378:metabolism; D004583:Electrons; D013294:Lactococcus lactis / Q000502:physiology*; D027361:Organic Anion Transporters / Q000235:genetics*; D010957:Plasmids / Q000032:analysis*; D012997:Solvents / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D027361:Organic Anion Transporters; D012997:Solvents; C065431:citP protein, Lactococcus lactis; D003931:Diacetyl