title: [Distribution of neuronal nitric oxide synthase-immunopositive neurons in rat corpus striatum and their ultrastructures].
abstract: OBJECTIVE
  To observe the distribution of neuronal nitric oxide synthase (nNOS)-immunopositive neurons in rat corpus striatum and their ultrastructural features.
  
  
  METHODS
  Brain tissue specimens were obtained from normal SD rats, in which nNOS-immunopositive neurons were visualized by ABC immunocytochemistry and observed under immunoelectron microscope with pre-embedding staining.
  
  
  RESULTS
  Under light microscope, nNOS-immunopositive neurons appeared brown with distinct profiles of the cell body and processes. These neurons, mostly medium-sized and small cells, were located mainly in the lateral region of the corpus striatum. Only a few immunopositive neurons were detected in the medial region of the corpus striatum. Immunohistochemistry and transmission electron microscopy identified the nNOS-immunopositive neurons as interneurons possessing large nuclei with small amount of cytoplasma. The immunopositive granules were visualized as black plaques, and the larger ones distributed mainly in the cell bodies, some with monolayer membrane encapsulation. The small granules did not have the encapsulation, scattering in perinuclear regions and under the cell membrane, but not in the cell body. The immunopositive granules were also found in the axons and dendrites, but not in the vesicles of the synapses. In addition, many immunopositive terminals were found close to the blood vessels.
  
  
  CONCLUSIONS
  nNOS-immunopositive neurons in rat corpus striatum are mainly medium-sized and small cells as is typical of the interneurons. The immunopositive granules locate in the cytoplasma, axons and dendrites, and larger granules have membrane coating while small ones do not, possibly in relation to their functions.
authors.affiliation: Institute of Clinical Anatomy, Key Laboratory of Tissue Construction and Detection of Guangdong Province, Southern Medical University, Guangzhou 510515, China. tgqguo@jnu.edu.cn
authors.forename: Guo-Qing
authors.initials: GQ
authors.lastname: Guo
authors.forename: Ai-Jing
authors.initials: AJ
authors.lastname: Shan
authors.forename: Wei-Zai
authors.initials: WZ
authors.lastname: Shen
authors.forename: Xiao-Zhong
authors.initials: XZ
authors.lastname: Qiu
authors.forename: Lei
authors.initials: L
authors.lastname: Yu
authors.forename: Jian-Qiang
authors.initials: JQ
authors.lastname: Qin
authors.forename: Jun
authors.initials: J
authors.lastname: Ouyang
authors.forename: Hua
authors.initials: H
authors.lastname: Liao
authors.forename: Shi-Zhen
authors.initials: SZ
authors.lastname: Zhong
mesh_terms: D000818:Animals; D003342:Corpus Striatum / Q000201:enzymology* / Q000648:ultrastructure; D007150:Immunohistochemistry; D008297:Male; D046529:Microscopy, Electron, Transmission; D009474:Neurons / Q000201:enzymology* / Q000648:ultrastructure; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D052248:Nitric Oxide Synthase Type I