Tyrosine nitration is a novel post-translational modification occurring on the neural intermediate filament protein peripherin.
The biological implication of protein tyrosine nitration in signaling pathways triggered by nitric oxide is recently emerging. Here we report for the first time that nitrotyrosination occurs in the neural intermediate filament protein peripherin. In neuron-like PC12 cells, nitrated peripherin is associated with the cytoskeleton fraction, its level increases during the progression of NGF-induced differentiation and the nitrated protein remains closely associated with stable microtubules. Tyr 17 and Tyr 376 were identified by MALDI-TOF analyses as two specific residues endogenously nitrated. Finally, peripherin nitration is not restricted to PC12 cells but it is also present in vivo in rat brain.
Tedeschi
Gabriella
G
Section of Biochemistry, University of Milano, Via Celoria 10, 20100 Milano, Italy. gabriella.tedeschi@unimi.it
Cappelletti
Graziella
G
Nonnis
Simona
S
Taverna
Francesca
F
Negri
Armando
A
Ronchi
Cristina
C
Ronchi
Severino
S
D000199:Actins / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D007381:Intermediate Filament Proteins / Q000737:chemistry* / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000378:metabolism*; D016716:PC12 Cells; D064531:Peripherins; D011499:Protein Processing, Post-Translational; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism
D000199:Actins; D007381:Intermediate Filament Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D064531:Peripherins; C002744:3-nitrotyrosine; D014443:Tyrosine