The conserved carboxyl terminus of human parainfluenza virus type 2 V protein plays an important role in virus growth.
Our previous results have shown that some residues of V protein-specific domain in human parainfluenza virus type 2 (hPIV2) are essential not only for STAT protein degradation but also for promoting virus growth. Here, we demonstrated that the virus growth of these recombinant hPIV2s (rPIV2) expressing mutated V proteins were improved in HeLa cell transiently expressing the wild-type V protein, but not in the cells constitutively expressing it. Consequently, we identified the region of the V protein that is essential for its oligomerization and for complex formation with NP protein. We also identified a host protein, AlP1/Alix, involved in apoptosis and efficient budding of several enveloped viruses as an interacting partner of the V and NP proteins. Depletion of AIP1/Alix by small interfering RNA suppressed virus growth. These data suggest that the conserved carboxyl terminus of the V protein plays an important role in virus growth.
Nishio
Machiko
M
Department of Microbiology, Mie University Graduate School of Medicine, 2-174, Edobashi, Tsu, Mie, 514-8507, Japan. nishio@doc.medic.mie-u.ac.jp
Tsurudome
Masato
M
Ishihara
Hisamitsu
H
Ito
Morihiro
M
Ito
Yasuhiko
Y
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism; D002522:Chlorocebus aethiops; D056827:Endosomal Sorting Complexes Required for Transport; D005816:Genetic Complementation Test; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D009698:Nucleoproteins / Q000378:metabolism; D010223:Parainfluenza Virus 2, Human / Q000254:growth & development* / Q000378:metabolism; D011485:Protein Binding; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary; D034622:RNA Interference; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D056827:Endosomal Sorting Complexes Required for Transport; C407031:NP protein, Human parainfluenza virus 2; D009698:Nucleoproteins; C117020:PDCD6IP protein, human; C068854:V protein, human parainfluenza virus type 1; D014764:Viral Proteins