title: Drosophila cacophony channels: a major mediator of neuronal Ca2+ currents and a trigger for K+ channel homeostatic regulation.
abstract: The cacophony (cac) locus in Drosophila encodes a Ca2+ channel alpha subunit, but little is known about properties of cac-mediated currents and functional consequences of cac mutations in central neurons. We found that, in Drosophila cultured neurons, Ca2+ currents were mediated predominantly by the cac channels. The cac channels contribute to low- and high-threshold, fast- and slow-inactivating types of Ca2+ currents, take part in membrane depolarization, and strongly activate Ca2+-activated K+ current [I(K(Ca))]. In cac neurons, unexpectedly, voltage-activated transient K+ current I(A) is upregulated to a level that matches I(K(Ca)) reduction, implicating a homeostatic regulation that was mimicked by chronic pharmacological blockade of Ca2+ currents in wild-type neurons. Among K+ channel transcripts, Shaker mRNA levels were preferentially increased in cac flies. However, Ca2+ current expression levels remained unaltered in several K+ channel mutants, illustrating a key role of cac in developmental regulation of Drosophila neuronal excitability.
authors.affiliation: Department of Biological Sciences, University of Iowa, Iowa City, Iowa 52242, USA.
authors.forename: I-Feng
authors.initials: IF
authors.lastname: Peng
authors.forename: Chun-Fang
authors.initials: CF
authors.lastname: Wu
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000378:metabolism* / Q000502:physiology; D002478:Cells, Cultured; D004330:Drosophila; D029721:Drosophila Proteins / Q000502:physiology*; D006706:Homeostasis / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D015221:Potassium Channels / Q000378:metabolism* / Q000502:physiology
chemical_list: D015220:Calcium Channels; D029721:Drosophila Proteins; D015221:Potassium Channels; C472405:cac protein, Drosophila