title: Effect of interleukin-10 on the Paracoccidioides brasiliensis killing by gamma-interferon activated human neutrophils.
abstract: Paracoccidioidomycosis, a deep mycosis endemic in Latin America, is a chronic granulomatous disease caused by the fungus Paracoccidioides brasiliensis. Phagocytic cells play a critical role against this fungus, and several studies have shown the effects of activator and suppressive cytokines on macrophage and monocyte functions. However, studies on polymorphonuclear neutrophils (PMNs), that are the first cells recruited to the infection sites, are scarcer. Thus, the objective of this paper was to assess whether interleukin-10 (IL-10), a potent anti-inflammatory cytokine, is able to block the activity of IFN-gamma-activated human PMNs upon P. brasiliensis intracellular killing, in vitro. The results showed that IFN-gamma-activated PMNs have an effective fungicidal activity against the fungus. This activity was associated with the release of high levels of H(2)O(2), the metabolite involved in phagocytic cells antifungal activities. However, the concomitant incubation of these cells with IFN-gamma and IL-10 significantly blocked IFN-gamma activation. As a consequence, PMNs killing activity and H(2)O(2) release were inhibited. Together, our results show the importance of PMNs exposure to activator or suppressor cytokines in the early stages of paracoccidioidomycosis infection.
authors.affiliation: Department of Microbiology and Immunology, Biosciences Institute, São Paulo State University-UNESP, Brazil.
authors.forename: Diego Luís
authors.initials: DL
authors.lastname: Costa
authors.forename: Luciane Alarcão
authors.initials: LA
authors.lastname: Dias-Melicio
authors.forename: Michele Janegitz
authors.initials: MJ
authors.lastname: Acorci
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Bordon
authors.forename: Elisandra Garcia
authors.initials: EG
authors.lastname: Tavian
authors.forename: Maria Terezinha Serrão
authors.initials: MT
authors.lastname: Peraçoli
authors.forename: Angela Maria Victoriano de Campos
authors.initials: AM
authors.lastname: Soares
mesh_terms: D000328:Adult; D006801:Humans; D006861:Hydrogen Peroxide / Q000276:immunology; D007371:Interferon-gamma / Q000037:antagonists & inhibitors* / Q000276:immunology*; D016753:Interleukin-10 / Q000276:immunology*; D008297:Male; D050296:Microbial Viability; D008875:Middle Aged; D009504:Neutrophils / Q000276:immunology*; D010228:Paracoccidioides / Q000276:immunology*
chemical_list: D016753:Interleukin-10; D007371:Interferon-gamma; D006861:Hydrogen Peroxide