title: Thermal stability of lysozyme Langmuir-Schaefer films by FTIR spectroscopy.
abstract: Fourier transform infrared spectroscopy has been applied to study the thermal stability of multilayer Langmuir-Schaefer (LS) films of lysozyme deposited on silicon substrates. The study has confirmed previous structural findings that the LS protein films have a high thermal stability that is extended in a lysozyme multilayer up to 200 degrees C. 2D infrared analysis has been used here to identify the correlated molecular species during thermal denaturation. Asynchronous 2D spectra have shown that the two components of water, fully and not fully hydrogen bonded, in the high-wavenumber range (2800-3600 cm-1) are negatively correlated with the amine stretching band at 3300 cm-1. On the grounds of the 2D spectra the FTIR spectra have been deconvoluted using three main components, two for water and one for the amine. This analysis has shown that, at the first drying stage, up to 100 degrees C, only the water that is not fully hydrogen bonded is removed. Moreover, the amine intensity band does not change up to 200 degrees C, the temperature at which the structural stability of the multilayer lysozyme films ceases.
authors.affiliation: Fondazione ELBA, Piazza Santissimi Apostoli 66, 00187 Rome, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Pechkova
authors.forename: P
authors.initials: P
authors.lastname: Innocenzi
authors.forename: L
authors.initials: L
authors.lastname: Malfatti
authors.forename: T
authors.initials: T
authors.lastname: Kidchob
authors.forename: L
authors.initials: L
authors.lastname: Gaspa
authors.forename: C
authors.initials: C
authors.lastname: Nicolini
mesh_terms: D000327:Adsorption; D000588:Amines; D004795:Enzyme Stability; D006860:Hydrogen Bonding; D009113:Muramidase / Q000737:chemistry*; D011489:Protein Denaturation; D012825:Silicon; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014867:Water
chemical_list: D000588:Amines; D014867:Water; D009113:Muramidase; D012825:Silicon