Quantification of foot and mouth disease virus excretion and transmission within groups of lambs with and without vaccination.
Sheep are well known to be susceptible for foot and mouth disease virus (FMDV), but it is unknown whether the infection can spread and persist in a sheep population. We therefore quantified virus transmission by performing experiments with FMD virus strain O/NET/2001 in groups of lambs. We used six groups of four lambs each, in which half of each group was inoculated and the other half was contact-exposed. To quantify the effectiveness of a single vaccination we also included six groups of four lambs each, vaccinated with O Manisa vaccine, 14 days prior to inoculation. Oropharyngeal fluid was obtained with a swab (OPF-swab), and blood samples were collected daily to determine virus excretion and serological response. We calculated the transmission rate beta (the number of new infections per day per infectious animal), and the reproduction ratio R (the number of secondary infections caused by one infectious individual). The mean daily virus excretion and the number of days the lambs excreted virus in the OPF differed significantly between vaccinated and non-vaccinated lambs. The transmission rate beta in the unvaccinated groups was 0.105 (95% confidence limit 0.044; 0.253) per day. The duration of the infectious period (T) was 21.11 (95% confidence limit 10.6; 42.1) days. With the final size of infection we estimated the reproduction ratio R in the non-vaccinated groups to be 1.14 (0.3; 3.3), and in vaccinated groups 0.22 (0.01; 1.78). Virus transmission as quantified by the final size did not differ statistically between the vaccinated and the non-vaccinated groups of lambs. In conclusion, FMDV seems able to persist in a sheep population, although the reproduction ratio was only slightly larger than one. As a consequence, vaccination might only have a small effect on transmission, in spite of the fact that virus excretion and duration of virus excretion is significantly reduced after vaccination.
Orsel
K
K
Faculty of Veterinary Medicine, Department of Farm Animal Health, Utrecht University, The Netherlands. k.orsel@vet.uu.nl
Dekker
A
A
Bouma
A
A
Stegeman
J A
JA
de Jong
M C M
MC
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005536:Foot-and-Mouth Disease / Q000635:transmission*; D029544:Foot-and-Mouth Disease Virus / Q000302:isolation & purification*; D012756:Sheep; D014611:Vaccination; D014765:Viral Vaccines / Q000276:immunology*
D000914:Antibodies, Viral; D014765:Viral Vaccines