Characterization and expression patterns of Sinorhizobium meliloti tmRNA (ssrA).
tmRNA (ssrA) in Sinorhizobium meliloti is a small RNA annotated by homology with the Bradyrhizobium japonicum sra molecule. Here, this molecule is described in Sinorhizobium meliloti as a model for such molecules in Alphaproteobacteria subgroup-2. Northern blot analysis and mapping of both 5' and 3' ends of this tmRNA allow the identification of two pieces: a 214 nt mRNA-like domain and an 82 nt tRNA-like domain, both highly stable, whereas the premature form is unstable. Transcriptional studies reveal that Sinorhizobium meliloti tmRNA is mainly expressed during growth resumption, replication initiation and various stress responses.
Ulvé
Vincent M
VM
CNRS UMR 6061-Génétique et Développement, Groupe Modèles Génétiques, Université de Rennes 1, Faculté de médecine, Rennes, France.
Chéron
Angélique
A
Trautwetter
Annie
A
Fontenelle
Catherine
C
Barloy-Hubler
Frédérique
F
D015152:Blotting, Northern; D004261:DNA Replication; D004777:Environment; D012329:RNA, Bacterial / Q000737:chemistry / Q000378:metabolism*; D017423:Sequence Analysis, RNA; D016962:Sinorhizobium meliloti / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D014158:Transcription, Genetic
D012329:RNA, Bacterial; C096222:tmRNA