Two-dimensional ultra-thin-layer chromatography and atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry in bioanalysis.
The feasibility of ultra-thin-layer chromatography (UTLC) and atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry (AP-MALDI-MS) for bioanalysis was studied with benzodiazepines as model substances in human urine. Two-dimensional (2D) UTLC was shown to be an efficient technique for the separation of benzodiazepines. Separations occurred in 4-12 min, and the separated compounds were identified by AP-MALDI-MS. The limits of detection with AP-MALDI-MS and AP-MALDI-MS/MS were in the picomole range and thus low enough for bioanalysis. The applicability of the 2D UTLC-AP-MALDI-MS was demonstrated in detection of metabolites with an authentic biological urine sample.
Salo
Piia K
PK
Faculty of Pharmacy, Division of Pharmaceutical Chemistry, and Drug Discovery and Development Technology Center, University of Helsinki, Helsinki, Finland.
Vilmunen
Suvi
S
Salomies
Hannele
H
Ketola
Raimo A
RA
Kostiainen
Risto
R
D001274:Atmospheric Pressure; D001569:Benzodiazepines / Q000302:isolation & purification / Q000652:urine*; D002855:Chromatography, Thin Layer / Q000379:methods*; D006801:Humans; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D001569:Benzodiazepines