title: Alkaloids of Hernandia voyronii: chloroquine-potentiating activity and structure elucidation of herveline D.
abstract: Further investigation of Hernandia voyronii led to the isolation of a new pavine-benzyltetrahydroisoquinoline (pavine-BTIQ) dimer, herveline D, together with herveline A, five aporphine alkaloids, two morphinane alkaloids, and their bio-synthetic precursor, i.e., the BTIQ ( S)-reticuline. Hervelines A-D have a moderate intrinsic in vitro antimalarial activity (IC (50) in the range of 1.68-3.28 microM), but displayed different effects ranging from synergism for herveline B and herveline C to simple additive effect for herveline A, and antagonism for herveline D in a chloroquine (CQ) combination evaluation and this was confirmed in vivo for hervelines A and B. Furthermore, the antiplasmoidal activity of CQ was potentiated in vitro by reticuline and its dimethyl derivative laudanosine (for the latter also in vivo), whereas herveline C moderately potentiated in vitro the anti-plasmodial activity of herveline D.
authors.affiliation: Institut Malgache de Recherches Appliquées, Antananarivo, Madagascar.
authors.forename: P
authors.initials: P
authors.lastname: Rasoanaivo
authors.forename: S
authors.initials: S
authors.lastname: Ratsimamanga-Urverg
authors.forename: H
authors.initials: H
authors.lastname: Rafatro
authors.forename: D
authors.initials: D
authors.lastname: Ramanitrahasimbola
authors.forename: G
authors.initials: G
authors.lastname: Palazzino
authors.forename: C
authors.initials: C
authors.lastname: Galeffi
authors.forename: M
authors.initials: M
authors.lastname: Nicoletti