title: Regionalized expression of ADAM13 during chicken embryonic development.
abstract: ADAMs are a family of membrane proteins possessing a disintegrin domain and a metalloprotease domain, which have functions in cell-cell adhesion, cell-matrix adhesion, and protein shedding, respectively. ADAMs are involved in morphogenesis and tissue formation during embryonic development. In the present study, chicken ADAM13 was cloned and identified, and its expression was investigated by semiquantitative reverse transcriptase-polymerase chain reaction and in situ hybridization during chicken embryonic development. Our results show that ADAM13 expression is temporally and spatially regulated in chicken embryos. At early developmental stages, ADAM13 is expressed in the head mesenchyme, which later develops into the craniofacial skeleton, in the branchial arches, and in the meninges surrounding the brain. Furthermore, ADAM13 mRNA was also detected in several tissues and organs, such as the somites and their derived muscles, the meninges surrounding the spinal cord, the dorsal aorta, the developing kidney, and several digestive organs.
authors.affiliation: Institute of Anatomy I, Friedrich Schiller University of Jena, Jena, Germany.
authors.forename: Juntang
authors.initials: J
authors.lastname: Lin
authors.forename: Christoph
authors.initials: C
authors.lastname: Redies
authors.forename: Jiankai
authors.initials: J
authors.lastname: Luo
mesh_terms: D051722:ADAM Proteins / Q000235:genetics*; D000818:Animals; D030161:Avian Proteins / Q000235:genetics*; D001921:Brain / Q000196:embryology / Q000378:metabolism; D002642:Chick Embryo; D002645:Chickens; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D007668:Kidney / Q000196:embryology / Q000378:metabolism; D008969:Molecular Sequence Data; D009432:Neural Crest / Q000196:embryology / Q000378:metabolism; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D019170:Somites / Q000378:metabolism
chemical_list: D030161:Avian Proteins; D018076:DNA, Complementary; D051722:ADAM Proteins