Chlamydial SET domain protein functions as a histone methyltransferase.
SET domain genes have been identified in numbers of bacterial genomes based on similarity to SET domains of eukaryotic histone methyltransferases. Herein, a Chlamydophila pneumoniae SET domain gene was clarified to be coincidently expressed with hctA and hctB genes encoding chlamydial histone H1-like proteins, Hc1 and Hc2, respectively. The SET domain protein (cpnSET) is localized in chlamydial cells and interacts with Hc1 and Hc2 through the C-terminal SET domain. As expected from conservation of catalytic sites in cpnSET, it functions as a protein methyltransferase to murine histone H3 and Hc1. However, little is known about protein methylation in the molecular pathogenesis of chlamydial infection. cpnSET may play an important role in chlamydial cell maturation due to modification of chlamydial histone H1-like proteins.
Murata
Masayuki
M
Department of Microbiology and Immunology, Yamaguchi University School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505, Japan.
Azuma
Yoshinao
Y
Department of Microbiology and Immunology, Yamaguchi University School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505, Japan.
Miura
Koshiro
K
Department of Clinical Research, National Sanyo Hospital, 685 Higashi-Kiwa, Ube, Yamaguchi 755-0241, Japan.|Department of Microbiology and Immunology, Yamaguchi University School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505, Japan.
Rahman
Mohd Akhlakur
MA
Department of Microbiology and Immunology, Yamaguchi University School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505, Japan.
Matsutani
Minenosuke
M
Department of Microbiology and Immunology, Yamaguchi University School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505, Japan.
Aoyama
Masahiro
M
Department of Microbiology and Immunology, Yamaguchi University School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505, Japan.
Suzuki
Harumi
H
Department of Microbiology and Immunology, Yamaguchi University School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505, Japan.
Sugi
Kazuro
K
Department of Clinical Research, National Sanyo Hospital, 685 Higashi-Kiwa, Ube, Yamaguchi 755-0241, Japan.
Shirai
Mutsunori
M
Department of Microbiology and Immunology, Yamaguchi University School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505, Japan.
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D016993:Chlamydophila pneumoniae / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D000076983:Histone Methyltransferases; D011495:Histone-Lysine N-Methyltransferase / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011496:Protein Methyltransferases; D015800:Protozoan Proteins / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism; D020798:Two-Hybrid System Techniques
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C088071:Hc1 protein, Chlamydia trachomatis; C082102:HctB protein, Chlamydia trachomatis; D006657:Histones; D015800:Protozoan Proteins; D016601:RNA-Binding Proteins; D000076983:Histone Methyltransferases; D011496:Protein Methyltransferases; D011495:Histone-Lysine N-Methyltransferase