Structure-activity relationship studies on quorum sensing ComX(RO-E-2) pheromone.
The ComX pheromone is a posttranslationally modified oligopeptide that stimulates natural genetic competence in Bacillus subtilis. Various ComX(RO-E-2) analogs were synthesized and their biological activities were studied to investigate structure-activity relationships. These results showed that the minimal active unit was the tripeptide, [3-5]ComX(RO-E-2), and all residues except the modified tryptophan residue were replaceable by alanine without total loss of activity.
Okada
Masahiro
M
Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan. okada-org@mail.tains.tohoku.ac,jp
Yamaguchi
Hisao
H
Sato
Isao
I
Cho
Soo Jeong
SJ
Dubnau
David
D
Sakagami
Youji
Y
D000409:Alanine / Q000737:chemistry; D019943:Amino Acid Substitution; D001407:Bacillus / Q000235:genetics; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D007763:Lac Operon / Q000235:genetics; D013058:Mass Spectrometry; D053038:Quorum Sensing / Q000187:drug effects*; D013329:Structure-Activity Relationship; D014364:Tryptophan / Q000737:chemistry
D001426:Bacterial Proteins; C087040:ComX protein, Bacillus subtilis; D007202:Indicators and Reagents; D014364:Tryptophan; D000409:Alanine