Novel method for preparing spheroplasts from cells with an internal cellulosic cell wall.
Protoplast and spheroplast preparations allow the transfer of macromolecules into cells and provide the basis for the generation of engineered organisms. Crypthecodinium cohnii cells harvested from polyethylene glycol-containing agar plates possessed significantly lower levels of cellulose in their cortical layers, which facilitated the delivery of fluorescence-labeled oligonucleotides into these cells.
Kwok
Alvin C M
AC
Department of Biology, Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong SAR, People's Republic of China.
Mak
Carmen C M
CC
Wong
Francis T W
FT
Wong
Joseph T Y
JT
D000818:Animals; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D002482:Cellulose / Q000096:biosynthesis*; D004141:Dinoflagellida / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification / Q000648:ultrastructure*; D016193:G1 Phase / Q000187:drug effects / Q000502:physiology; D016195:G2 Phase; D008560:Membrane Fluidity; D030781:Organisms, Genetically Modified; D010539:Permeability / Q000187:drug effects; D011092:Polyethylene Glycols / Q000494:pharmacology; D011523:Protoplasts / Q000187:drug effects / Q000378:metabolism; D013104:Spheroplasts / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification / Q000648:ultrastructure*; D013194:Staining and Labeling
D011092:Polyethylene Glycols; D002482:Cellulose