title: Thioredoxin prevents the development and progression of elastase-induced emphysema.
abstract: Thioredoxin 1 (TRX1) is a redox (reduction/oxidation)-active protein that scavenges reactive oxygen species. Here we examined whether endogenous or exogenous administration of TRX1 prevented the development and progression of elastase-induced pulmonary emphysema. Mice were treated with intratracheal elastase via microspray on day 0, and were given recombinant human TRX1 (rhTRX1) every other day from days -1 to 21. To determine the effects of TRX1 on the progression of established emphysema, mice were treated intratracheally with elastase on day 0, and rhTRX1 was administered from days 14 to 21. Histopathologic examination was performed on day 21. TRX1-transgenic but not transgene-negative mice demonstrated a decrease in the physiological indicators of elastase-induced emphysema. TRX1 administration from days -1 to 19 significantly decreased the signs of elastase-induced emphysema. Moreover, TRX1 administration beginning 14 days after elastase treatment significantly slowed the progression of emphysema. TRX1 may be of clinical benefit for the treatment of COPD.
authors.affiliation: Division of Respirology, Neurology, and Rheumatology, Department of Medicine, Kurume University School of Medicine, Kurume, Fukuoka 830-0011, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kinoshita
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Hoshino
authors.forename: Haruki
authors.initials: H
authors.lastname: Imaoka
authors.forename: Hiroko
authors.initials: H
authors.lastname: Ichiki
authors.forename: Masaki
authors.initials: M
authors.lastname: Okamoto
authors.forename: Tomotaka
authors.initials: T
authors.lastname: Kawayama
authors.forename: Junji
authors.initials: J
authors.lastname: Yodoi
authors.forename: Seiya
authors.initials: S
authors.lastname: Kato
authors.forename: Hisamichi
authors.initials: H
authors.lastname: Aizawa
mesh_terms: D000818:Animals; D018450:Disease Progression; D004646:Emphysema / Q000139:chemically induced / Q000517:prevention & control*; D051379:Mice; D008822:Mice, Transgenic; D010084:Oxidation-Reduction; D010196:Pancreatic Elastase; D029424:Pulmonary Disease, Chronic Obstructive / Q000517:prevention & control*; D011656:Pulmonary Emphysema / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D013879:Thioredoxins / Q000627:therapeutic use*; D013997:Time Factors
chemical_list: C515559:Txn1 protein, mouse; D013879:Thioredoxins; D010196:Pancreatic Elastase