title: Bacterial quantification in teeth with apical periodontitis related to instrumentation and different intracanal medications: a randomized clinical trial.
abstract: The antibacterial efficacy of intracanal medication with calcium hydroxide [Ca(OH)2], 2% chlorhexidine gel (CHX), and a combination of both [Ca(OH)2/CHX] was assessed in teeth with chronic apical periodontitis. Thirty-three canals were instrumented, randomly divided into three groups, and medicated with either Ca(OH)2, CHX, or Ca(OH)2/CHX. Bacteriological samples obtained from the operative field and the root canals before (S1) and after instrumentation (S2) in the first treatment session, and after medication (S3) in the second session 1 week later, were assessed for bacterial growth, observed by turbidity and in agar plates, and viable colony-forming unit (CFU) counts. Bacterial growth and CFU counts decreased significantly from S1 to S2 (Mann-Whitney, p<0.05). Differences in growth and counts between S2 to S3 were not statistically significant for all three intracanal medication groups. It was concluded that the antibacterial efficacy of Ca(OH)2, CHX, and Ca(OH)2/CHX was comparable.
authors.affiliation: Discipline of Endodontics, Faculty of Dentistry, University of Toronto, Ontario, Canada. aldomanzur@gmail.com
authors.forename: Aldo
authors.initials: A
authors.lastname: Manzur
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: González
authors.forename: Amaury
authors.initials: A
authors.lastname: Pozos
authors.forename: Daniel
authors.initials: D
authors.lastname: Silva-Herzog
authors.forename: Shimon
authors.initials: S
authors.lastname: Friedman
mesh_terms: D000293:Adolescent; D000328:Adult; D001421:Bacteria, Anaerobic / Q000187:drug effects*; D002126:Calcium Hydroxide / Q000494:pharmacology*; D002648:Child; D002710:Chlorhexidine / Q000494:pharmacology*; D002908:Chronic Disease; D015169:Colony Count, Microbial; D004338:Drug Combinations; D006801:Humans; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry; D010485:Periapical Periodontitis / Q000382:microbiology* / Q000628:therapy; D012388:Root Canal Irrigants / Q000494:pharmacology*; D018915:Root Canal Preparation / Q000295:instrumentation / Q000379:methods; D018709:Statistics, Nonparametric
chemical_list: D004338:Drug Combinations; D012388:Root Canal Irrigants; D002126:Calcium Hydroxide; D002710:Chlorhexidine