Production and identification of a novel compound, 7,10-dihydroxy-8(E)-hexadecenoic acid from palmitoleic acid by Pseudomonas aeruginosa PR3.
Hydroxy fatty acids are considered as important value-added product for industrial application because of their special properties such as higher viscosity and reactivity. Microbial production of the hydroxy fatty acids from various fatty acid substrates have been actively studied using several microorganisms. The new bacterial isolate Pseudomonas aeruginosa (PR3) had been reported to produce mono-, di-, and tri-hydroxy fatty acids from different unsaturated fatty acids. Of those, 7,10-dihydroxy-8(E)-octadecenoic acid (DOD) and 7,10,12-trihydroxy-8(E)-octadecenoic acid (TOD) were produced from oleic acid and ricinoleic acid, respectively. Based on the postulated common metabolic pathway involved in DOD and TOD formation by PR3, it was assumed that palmitoleic acid containing a singular 9-cis double bond, common structural property sharing with oleic acid and ricinoleic acid, could be utilized by PR3 to produce hydroxy fatty acid. In this study, we tried to use palmitoleic acid as substrate for production of hydroxy fatty acid by PR3 and firstly confirmed that PR3 could produce 7,10-dihydroxy-8(E)-hexadecenoic acid (DHD) with 23% yield from palmitoleic acid. DHD production was peaked at 72 h after the substrate was added to the 24-h-culture.
Bae
Jae-Han
JH
Department of Animal Science and Biotechnology, Kyungpook National University, Daegu, 702-701, South Korea.
Kim
Deuk-Soo
DS
Suh
Min-Jung
MJ
Oh
Sei-Ryang
SR
Lee
In-Jung
IJ
Kang
Sun-Chul
SC
Hou
Ching T
CT
Kim
Hak-Ryul
HR
D001709:Biotechnology / Q000379:methods*; D003470:Culture Media; D005229:Fatty Acids, Monounsaturated / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D009829:Oleic Acids / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000378:metabolism*
C477043:7,10-dihydroxy-8-octadecenoic acid; D003470:Culture Media; D005229:Fatty Acids, Monounsaturated; D009829:Oleic Acids; C008757:palmitoleic acid