title: Morphometric and some immunohistochemical characteristics of human choroids plexus stroma and psammoma bodies.
abstract: Psammoma bodies (PBs) are one of many choroids plexus aging changes. The aim of our research was to perform the quantification of PBs' presence in human choroids plexus stroma, as well as to evaluate the characteristics of choroids plexus stroma in cases in which PBs were present. Afterwards, the observations of the histochemical analysis would be confirmed by immunohistochemical analysis. Choroid plexuses of 30 cadavers were used for the histochemical and, choroids plexuses of 15 cadavers in which PBs' presence was confirmed during the histochemical analysis, were used as material for the immunohistochemical analysis. Light microscopy, histochemical, immunohistochemical, and morphometric method were applied during the study. Classification of the cases was performed by cluster analysis. We observed increase of choroids plexus PBs' presence during the aging process. But this increase is not linear. Their presence is the largest in the second cluster that is younger than the third and older than the first. Nuclear morphometric parameters of the stroma in these cases showed that the cellular composition in this cluster is different than in other two and, that contain larger number of lymphoid cells. Immunohistochemical analysis showed PBs' positive reaction on vimentin, CD45R0, and LCA markers, while in their vicinity, as well as inside them, numerous T-cells were observed. So, the presence of CD45R0 and LCA-positive T cells, PBs' positive reaction on the same markers, indirectly connect these cells with PBs' formation process.
authors.affiliation: Department of Anatomy, Medical Faculty, University of Nis, Nis, Serbia. ivanjov@junis.ni.ac.yu
authors.forename: Ivan
authors.initials: I
authors.lastname: Jovanović
authors.forename: Sladjana
authors.initials: S
authors.lastname: Ugrenović
authors.forename: Svetlana
authors.initials: S
authors.lastname: Antić
authors.forename: Natalija
authors.initials: N
authors.lastname: Stefanović
authors.forename: Dragan
authors.initials: D
authors.lastname: Mihailović
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging; D002113:Calcification, Physiologic; D002490:Central Nervous System / Q000098:blood supply / Q000166:cytology* / Q000502:physiology; D002831:Choroid Plexus / Q000737:chemistry / Q000502:physiology / Q000648:ultrastructure*; D006801:Humans; D007150:Immunohistochemistry; D002479:Inclusion Bodies / Q000737:chemistry* / Q000648:ultrastructure*; D008875:Middle Aged; D017154:Stromal Cells / Q000737:chemistry / Q000648:ultrastructure*; D014746:Vimentin / Q000032:analysis
chemical_list: D014746:Vimentin