title: Nitrate reductase-mediated synthesis of silver nanoparticles from AgNO3.
abstract: Synthesis of silver nanoparticles using alpha-NADPH-dependent nitrate reductase and phytochelatin in vitro has been demonstrated for the first time. The silver ions were reduced in the presence of nitrate reductase, leading to the formation of a stable silver hydrosol 10-25 nm diam. and stabilized by the capping peptide. The nanoparticles were characterized by X-ray diffraction, transmission electron microscopy, X-ray photoelectron spectroscopy and UV-Vis absorption. These studies will help in designing a rational enzymatic strategy for the synthesis of nanomaterials of different chemical composition, shapes and sizes as well as their separation.
authors.affiliation: Biochemical Sciences Division, National Chemical Laboratory, Pune, India.
authors.forename: S
authors.initials: S
authors.lastname: Anil Kumar
authors.forename: Majid Kazemian
authors.initials: MK
authors.lastname: Abyaneh
authors.forename: S W
authors.initials: SW
authors.lastname: Gosavi
authors.forename: Sulabha K
authors.initials: SK
authors.lastname: Kulkarni
authors.forename: Renu
authors.initials: R
authors.lastname: Pasricha
authors.forename: Absar
authors.initials: A
authors.lastname: Ahmad
authors.forename: M I
authors.initials: MI
authors.lastname: Khan
mesh_terms: D003460:Crystallization / Q000379:methods; D005670:Fusarium / Q000201:enzymology*; D008968:Molecular Conformation; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods*; D050901:Nitrate Reductase / Q000737:chemistry*; D010316:Particle Size; D012834:Silver / Q000737:chemistry*; D012835:Silver Nitrate / Q000737:chemistry*
chemical_list: D012834:Silver; D012835:Silver Nitrate; D050901:Nitrate Reductase