Effect of Bauhinia racemosa stem bark on N-nitrosodiethylamine-induced hepatocarcinogenesis in rats.
The aim of this study is to investigate the antioxidant defense system induced by the methanol extract of Bauhinia racemosa L.(MEBR) against N-nitrosodiethylamine (NDEA)-induced hepatocarcinogenesis in Wister albino rats. The effects of MEBR on surface visible macroscopic (Morphometry) liver lesions (neoplastic nodules) and the levels of serum enzymes, lipid peroxidation and antioxidants were evaluated in NDEA-induced hepatocarcinogenesis in rats. In rats treated, with NDEA, significantly elevated levels of serum enzymes (SGOT, SGPT and ALP), bilirubin and decreased levels of protein and uric acid were observed. Significantly elevated amount of malondialdehyde (MDA), the end product of lipidperoxidation, indicated higher levels of lipid peroxidation, which was accompanied by significantly decreased levels of antioxidants like vitamin C, vitamin E, reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT). Administration of MEBR was able to suppress nodule development/hepatocellular lesion formation in rats. The extract treatment increases in antioxidant levels and dramatic decreases in lipid peroxidation levels. MEBR also produced a protective effect by decreasing the level of serum enzymes, bilirubin and increased the protein and uric acid levels. The results suggest that MEBR exert chemopreventive effects by suppressing nodule development and decreasing lipid peroxidation and enhancing the levels of antioxidants in NDEA carcinogenesis by reducing the formation of free radicals.
Kumar
Ramanathan Sambath
RS
Natural Products Research Laboratories, JKK Nataraja College of Pharmacy, Komarapalayam, Namakkal-638 183, Tamilnadu, India. sambathju2002@yahoo.co.in
Sunderam
Rajagopal Shanmuga
RS
Sivakumar
Thangavel
T
Sivakumar
Palanivel
P
Sureshkumar
Raman
R
Kanagasabi
Rengaswamy
R
Vijaya
Manickum
M
Perumal
Baskaran Perumal
BP
Gupta
Malaya
M
Mazumdar
Upal Kanti
UK
Kumar
Mani Senthil
MS
Kumar
Kuppuswamy Asok
KA
D000410:Alanine Transaminase / Q000097:blood; D000477:Alkylating Agents; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001219:Aspartate Aminotransferases / Q000097:blood; D036481:Bauhinia; D001663:Bilirubin / Q000097:blood; D006528:Carcinoma, Hepatocellular / Q000139:chemically induced / Q000517:prevention & control*; D018890:Chemoprevention; D004052:Diethylnitrosamine; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008113:Liver Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008517:Phytotherapy / Q000379:methods*; D024301:Plant Bark; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar
D000477:Alkylating Agents; D000975:Antioxidants; D010936:Plant Extracts; D004052:Diethylnitrosamine; D008315:Malondialdehyde; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D001663:Bilirubin