title: Augmentation of 11beta-hydroxysteroid dehydrogenase type 1 in LPS-activated J774.1 macrophages--role of 11beta-HSD1 in pro-inflammatory properties in macrophages.
abstract: Macrophage infiltration in obese adipose tissue provokes local inflammation and insulin resistance. Evidence has accumulated that activation of 11beta-HSD1 in adipocytes is critically involved in dysfunction of adipose tissue. However, the potential role of 11beta-HSD1 in macrophages still remains unclear. We here demonstrate that a murine macrophage cell line, J774.1 cells expressed 11beta-HSD1 mRNA and reductase activity, both of which were augmented by lipopolysaccharide (LPS)-induced cell activation. Three kinds of pharmacological inhibition of 11beta-HSD1 in LPS-treated macrophages significantly suppressed the expression and secretion of interleukin 1beta, tumor necrosis factor alpha or monocyte chemoattractant protein 1, thereby highlighting a novel role of 11beta-HSD1 in pro-inflammatory properties of activated macrophages.
authors.affiliation: Division of Endocrinology and Metabolism, Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, 54 Shogoin Kawaharacho, Sakyoku, Kyoto 606-8507, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Ishii
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Masuzaki
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Tanaka
authors.forename: Naoki
authors.initials: N
authors.lastname: Arai
authors.forename: Shintaro
authors.initials: S
authors.lastname: Yasue
authors.forename: Nozomi
authors.initials: N
authors.lastname: Kobayashi
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Tomita
authors.forename: Michio
authors.initials: M
authors.lastname: Noguchi
authors.forename: Junji
authors.initials: J
authors.lastname: Fujikura
authors.forename: Ken
authors.initials: K
authors.lastname: Ebihara
authors.forename: Kiminori
authors.initials: K
authors.lastname: Hosoda
authors.forename: Kazuwa
authors.initials: K
authors.lastname: Nakao
mesh_terms: D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1 / Q000235:genetics / Q000378:metabolism*; D017667:Adipocytes / Q000502:physiology; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D003907:Dexamethasone / Q000494:pharmacology; D007249:Inflammation / Q000473:pathology / Q000503:physiopathology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology*; D051379:Mice; D009765:Obesity / Q000473:pathology / Q000503:physiopathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D017931:DNA Primers; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D003907:Dexamethasone; D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1