Studies on the production of enantioselective nitrilase in a stirred tank bioreactor by Pseudomonas putida MTCC 5110.
Nitrilases constitute an important class of hydrolases, having numerous industrial applications. The present work aims to address the production of nitrile hydrolyzing enzymes from Pseudomonas putida MTCC 5110 in a 6l bioreactor. Effect of various physico-chemical conditions and process parameters like pH, temperature, aeration and agitation rates and inducer concentration was studied. Further, the enzyme activity was enhanced by adopting the inducer feeding strategy. Various biochemical engineering parameters pertaining to the cultivation of P. putida in different physico-chemical conditions were reported. Finally, segregation of growth phase from the enzyme production phase allowed significant reduction in total fermentation time.
Naik
Subhash Chandra
SC
Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector-67, SAS Nagar, Mohali, Punjab, India.
Kaul
Praveen
P
Barse
Brajesh
B
Banerjee
Anirban
A
Banerjee
U C
UC
D000097:Acetonitriles / Q000378:metabolism; D000619:Aminohydrolases / Q000032:analysis / Q000096:biosynthesis*; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D011108:Polymers / Q000378:metabolism; D011409:Propylene Glycols / Q000378:metabolism; D016958:Pseudomonas putida / Q000201:enzymology*; D013237:Stereoisomerism; D013696:Temperature; D013997:Time Factors
D000097:Acetonitriles; D003470:Culture Media; D011108:Polymers; D011409:Propylene Glycols; C012504:polypropylene glycol; D000619:Aminohydrolases; C021867:nitrilase