Amplification and translocation of 3q26 with overexpression of EVI1 in Fanconi anemia-derived childhood acute myeloid leukemia with biallelic FANCD1/BRCA2 disruption.
Fanconi anemia (FA) is an inherited disease with congenital abnormalities and an extreme risk of acute myeloid leukemia (AML). Genetic events occurring during malignant transformation in FA and the biology of FA-associated AML are poorly understood, but are often preceded by the development of chromosomal aberrations involving 3q26-29 in bone marrow of FA patients. We report here the molecular cytogenetic characterization of FA-derived AML cell lines SB1685CB and SB1690CB by conventional and array comparative genomic hybridization, fluorescence in situ hybridization, and SKY. We identified gains of a 3.7 MB chromosomal region on 3q26.2-26.31, which preceded transformation to overt leukemia. This region harbors the oncogenic transcription factor EVI1. A third FA-derived cell line, FA-AML1, carried a translocation with ectopic localization of 3q26 including EVI1. Rearrangements of 3q, which are rare in childhood AML, commonly result in overexpression of EVI1, which determines specific gene expression patterns and confers poor prognosis. We detected overexpression of EVI1 in all three FA-derived AML. Our results suggest a link between the FA defect, chromosomal aberrations involving 3q and overexpression of EVI1. We hypothesize that constitutional or acquired FA defects might be a common factor for the development of 3q abnormalities in AML. In addition, cryptic imbalances as detected here might account for overexpression of EVI1 in AML without overt 3q26 rearrangements.
Meyer
Stefan
S
Academic Unit of Paediatric Oncology, Christie Hospital Trust, University of anchester, Manchester, UK. stefan.meyer@manchester.ac.uk
Fergusson
William D
WD
Whetton
Anthony D
AD
Moreira-Leite
Flavia
F
Pepper
Stuart D
SD
Miller
Crispin
C
Saunders
Emma K
EK
White
Daniel J
DJ
Will
Andrew M
AM
Eden
Tim
T
Ikeda
Hideyuki
H
Ullmann
Reinhard
R
Tuerkmen
Seval
S
Gerlach
Antje
A
Klopocki
Eva
E
Tönnies
Holger
H
D000208:Acute Disease; D024682:BRCA2 Protein / Q000235:genetics*; D002460:Cell Line; D002648:Child; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D005199:Fanconi Anemia / Q000235:genetics*; D005784:Gene Amplification; D006801:Humans; D007951:Leukemia, Myeloid / Q000235:genetics*; D000074008:MDS1 and EVI1 Complex Locus Protein; D011519:Proto-Oncogenes / Q000235:genetics*; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*; D014178:Translocation, Genetic
D024682:BRCA2 Protein; D004268:DNA-Binding Proteins; D000074008:MDS1 and EVI1 Complex Locus Protein; C063575:MECOM protein, human; D014157:Transcription Factors