title: Gas chromatography-mass spectrometric assay for propofol in cerebrospinal fluid of traumatic brain patients.
abstract: A sensitive gas chromatography-mass spectrometry method for measuring propofol in cerebrospinal fluid is described, validated and applied to four patients after traumatic brain injury. The limit of quantitation was 2 microg/L using a volume of 0.5 mL. The inter- and intra-assay coefficients of variation were 5.9 and 5.1%, respectively. The assay covers the linear concentration range of 2-50 microg/L, which is relevant in clinical pharmacokinetic studies when propofol is used in the Intensive Care Unit as a sedative agent or to lower the intracranial pressure.
authors.affiliation: Department of Clinical Pharmacy, St. Antonius Hospital, P.O. Box 2500, 3430 EM Nieuwegein, The Netherlands. r.peeters@antoniusmesosgroep.nl
authors.forename: Mariska Y M
authors.initials: MY
authors.lastname: Peeters
authors.forename: Hiltjo
authors.initials: H
authors.lastname: Kuiper
authors.forename: Ben
authors.initials: B
authors.lastname: Greijdanus
authors.forename: Joukje
authors.initials: J
authors.lastname: van der Naalt
authors.forename: Catherijne A J
authors.initials: CA
authors.lastname: Knibbe
authors.forename: Donald R A
authors.initials: DR
authors.lastname: Uges
mesh_terms: D000293:Adolescent; D000328:Adult; D018686:Anesthetics, Intravenous / Q000134:cerebrospinal fluid*; D001930:Brain Injuries / Q000134:cerebrospinal fluid*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008297:Male; D015742:Propofol / Q000134:cerebrospinal fluid*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D018686:Anesthetics, Intravenous; D015742:Propofol