Investigation into the factors affecting accuracy of mass measurements on a time-of-flight mass spectrometer using Design of Experiment.
The results of an investigation of the parameters which have the most significant effect on the accuracy of mass measurements on a quadrupole orthogonal acceleration time-of-flight mass spectrometer (q-oaToF) are reported. The influence of eight factors is investigated: ion abundances of reference and analyte compounds, mass difference between analyte and reference compounds, quality of calibration, number of reference acquisitions averaged and TDC (time-to-digital converter) settings (resolution, Np multiplier (number of pushes correction factor), minimum number of points, i.e. minimum acquisition width which defines a peak). To extract the maximum information from as few experiments as possible, a Design of Experiment approach was used. The data will be used as a basis for developing guidance on accurate mass measurement on q-oaToF instruments.
Laures
Alice M-F
AM
Analytical Sciences, GlaxoSmithKline, Gunnels Wood Road, Stevenage SG1 2NY, UK. alice.2.laures@gsk.com
Wolff
Jean-Claude
JC
Eckers
Christine
C
Borman
Phil J
PJ
Chatfield
Marion J
MJ
D006635:Histamine H2 Antagonists / Q000737:chemistry*; D011899:Ranitidine / Q000737:chemistry*; D015203:Reproducibility of Results; D012107:Research Design; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D006635:Histamine H2 Antagonists; D011899:Ranitidine