Supramolecular-mediated immobilization of L-phenylalanine dehydrogenase on cyclodextrin-coated Au electrodes for biosensor applications.
A supramolecular approach was used for adsorbing a monolayer of adamantane-modified phenylalanine dehydrogenase on beta-cyclodextrin-coated Au electrodes. The enzyme electrode (poised at +200 mV vs. Ag/AgCl) showed a linear amperometric response up to 3 mM L-phenylalanine (L-Phe) with a lower detection limit of 15 microM. The reversible nature of this immobilization approach was confirmed.
Villalonga
Reynaldo
R
Center for Enzyme Technology, University of Matanzas, Matanzas, Cuba. reynaldo.villalonga@umcc.cu
Fujii
Akira
A
Shinohara
Hiroaki
H
Asano
Yasuhisa
Y
Cao
Roberto
R
Tachibana
Shinjiro
S
Ortiz
Pedro
P
D000594:Amino Acid Oxidoreductases / Q000737:chemistry*; D001407:Bacillus / Q000201:enzymology; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation*; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry; D008839:Microelectrodes; D046912:Multiprotein Complexes / Q000737:chemistry; D010649:Phenylalanine / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D047392:beta-Cyclodextrins / Q000737:chemistry*
D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D046912:Multiprotein Complexes; D047392:beta-Cyclodextrins; D010649:Phenylalanine; D006046:Gold; D000594:Amino Acid Oxidoreductases; C022342:phenylalanine oxidase; C031215:betadex