Unexpected altered specificity is responsible for St. Louis encephalitis virus recombinant protease autoproteolysis.
We report herein the study of the cleavage fragments generated by autoproteolysis of the St. Louis encephalitis virus recombinant protease. The cleavage sites leading to truncated forms were identified by microsequencing, which revealed an unexpected altered specificity of the recombinant proteinase towards unusual sequences.
Pastorino
Boris A M
BA
Unité de virologie tropicale, Institut de médecine tropicale du service de santé des armées, BP 46, 13 998 Marseille armées, France. publi.viro@laposte.net
Peyrefitte
Christophe N
CN
Grandadam
Marc
M
Lebrun
Régine
R
Moinier
Danielle
D
Rolland
Dominique
D
Tolou
Hugues J
HJ
Bessaud
Maël
M
D000595:Amino Acid Sequence; D004665:Encephalitis Virus, St. Louis / Q000201:enzymology*; D010450:Endopeptidases / Q000378:metabolism*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000378:metabolism*; D013379:Substrate Specificity
D011994:Recombinant Proteins; D010450:Endopeptidases