Intradermal immunization with ovalbumin-loaded poly-epsilon-caprolactone microparticles conferred protection in ovalbumin-sensitized allergic mice.
BACKGROUND
Although immunotherapy has been reported as the only treatment able to revert the T-helper type 2 (Th2) response, its administration has some disadvantages such as the requirement of multiple doses, possible side-effects provoked by conventional adjuvants and the risk of suffering an anaphylactic shock. For these reasons, drug-delivery systems appear to be a promising strategy due to its ability to (i) transport the allergens, (ii) protect them from degradation, (iii) decrease the number of administrations and (iv) act as immuno-adjuvants.


OBJECTIVE
The aim of this work was to evaluate the properties of poly-epsilon-caprolactone (PCL) microparticles as adjuvants in immunotherapy using ovalbumin (OVA) as an allergen model. For this purpose, the protection capacity of these microparticles (OVA PCL) against OVA allergy was studied in a murine model.


METHODS
The humoral and cellular-induced immune response generated by OVA encapsulated into PCL microparticles was studied by immunizing BALB/c mice intradermically. Also, OVA-sensitized mice were treated with OVA PCL and OVA adsorbed to aluminium hydroxide (OVA-Alum). Fifteen days after therapy, animals were challenged with OVA and different signs of anaphylactic shock were evaluated.


RESULTS
One single shot by an intradermal route with OVA PCL resulted in a Th2-type immune response. In OVA-sensitized mice, treatment with OVA PCL elicited high OVA-specific IgG but low levels of IgE. Furthermore, OVA PCL mice group displayed lower levels of serum histamine and higher survival rate in comparison with the positive control group.


CONCLUSIONS
The anaphylactic shock suffered by OVA PCL-treated mice was weaker than the one induced in the OVA-Alum group. Hence, the intradermal immunization with OVA PCL microparticles induced hyposensitization in OVA-allergic mice.
Roman
B S
BS
Immunoadjuvant Unit, Department of Pharmaceutical Technology, University of Navarra, Pamplona, Spain.
Espuelas
S
S
Gómez
S
S
Gamazo
C
C
Sanz
M L
ML
Ferrer
M
M
Irache
J M
JM
D000485:Allergens / Q000276:immunology*; D000707:Anaphylaxis / Q000276:immunology*; D000818:Animals; D004333:Drug Administration Routes; D006801:Humans; D007108:Immune Tolerance / Q000276:immunology*; D051379:Mice; D010047:Ovalbumin / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*
D000485:Allergens; D010047:Ovalbumin