title: Influence of the allelic variants encoded at the Gli-B1 locus, responsible for a major allergen of wheat, on IgE reactivity for patients suffering from food allergy to wheat.
abstract: Wheat presents an important genetic diversity that could be useful to look for cultivars with reduced allergencity. omega5-Gliadins have been described as major allergens for wheat allergic patients suffering from wheat-dependent exercise-induced anaphylaxis (WDEIA) and some cases of chronic urticaria (U). Our objective was to study the influence of genetic variability at the Gli-B1 locus encoding for omega5-gliadins on the reactivity of IgE antibodies from these patients. We selected cultivars expressing 13 alleles at Gli-B1 including a wheat/rye translocation and studied the reactivity to gliadins of a rabbit antiserum specific for omega5-gliadins and of IgE from 10 patients. The antiserum and IgE from nine patients with WDEIA and U strongly detected omega5-gliadins expressed by most of the Gli-B1 alleles but showed no or faint responses to the gliadins and secalins extracted from the translocated wheat. The selection of genotypes lacking the Gli-B1 locus may reduce wheat allergenicity.
authors.affiliation: Unité de Recherche sur les Biopolymères, leurs Interactions et Assemblages (BIA), INRA, Rue de la Géraudière, BP 71627, 44316 Nantes cedex 3, France. denery@nantes.inra.fr
authors.forename: Sandra
authors.initials: S
authors.lastname: Denery-Papini
authors.forename: Michel
authors.initials: M
authors.lastname: Lauriére
authors.forename: Gérard
authors.initials: G
authors.lastname: Branlard
authors.forename: Martine
authors.initials: M
authors.lastname: Morisset
authors.forename: Catherine
authors.initials: C
authors.lastname: Pecquet
authors.forename: Dominique
authors.initials: D
authors.lastname: Choudat
authors.forename: Marielle
authors.initials: M
authors.lastname: Merlino
authors.forename: Florence
authors.initials: F
authors.lastname: Pineau
authors.forename: Yves
authors.initials: Y
authors.lastname: Popineau
authors.forename: Elodie
authors.initials: E
authors.lastname: Boulenc
authors.forename: Isabelle
authors.initials: I
authors.lastname: Bouchez-Mahiout
authors.forename: Marie
authors.initials: M
authors.lastname: Bodinier
authors.forename: Denise-Anne
authors.initials: DA
authors.lastname: Moneret-Vautrin
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D000485:Allergens / Q000032:analysis / Q000235:genetics* / Q000276:immunology*; D000707:Anaphylaxis / Q000276:immunology; D000818:Animals; D052179:Antigens, Plant; D015444:Exercise; D005512:Food Hypersensitivity / Q000276:immunology*; D014644:Genetic Variation / Q000276:immunology*; D005903:Gliadin / Q000032:analysis / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology*; D008875:Middle Aged; D011817:Rabbits; D014908:Triticum / Q000737:chemistry / Q000276:immunology; D014581:Urticaria / Q000276:immunology
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D007106:Immune Sera; C475180:omega-5 gliadin, wheat; D007073:Immunoglobulin E; D005903:Gliadin