Mapping of KIT adjacent sequences on canid autosomes and B chromosomes.
B chromosomes are often considered to be one of the most mysterious elements of karyotypes (Camacho, 2004). It is generally believed that mammalian B chromosomes do not contain any protein coding genes. The discovery of a conserved KIT gene in Canidae B chromosomes has changed this view. Here we performed analysis of sequences surrounding KIT in B chromosomes of the fox and raccoon dog. The presence of the RPL23A pseudogene was shown in canid B chromosomes. The 3' end fragment of the KDR gene was found in raccoon dog B chromosomes. The size of the B-specific fragment homologous to the autosome fragment was estimated to be a minimum of 480 kbp in both species. The origin and evolution of B chromosomes in Canidae are discussed.
Yudkin
D V
DV
Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia.
Trifonov
V A
VA
Kukekova
A V
AV
Vorobieva
N V
NV
Rubtsova
N V
NV
Yang
F
F
Acland
G M
GM
Ferguson-Smith
M A
MA
Graphodatsky
A S
AS
D000818:Animals; D002871:Chromosome Banding; D002874:Chromosome Mapping / Q000379:methods*; D002875:Chromosomes / Q000648:ultrastructure*; D004285:Dogs; D005589:Foxes; D015723:Gene Library; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008957:Models, Genetic; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics*; D046031:Raccoon Dogs
D019009:Proto-Oncogene Proteins c-kit