[High performance liquid chromatography-mass spectrometry analysis of radix Angelica sciensis].
OBJECTIVE
To analyze the chemical components in Danggui (the roots of Angelica sinensis (Oliv.) Diel).


METHODS
HPLC-MS/MS was used to identify the main components in Danggui. Furthermore, the MS fragmentation regularity of the phthalides was proposed. The mobile phase of HPLC consisted of 0.5% acetic acid in water and 0.5% acetic acid in acetonitrile, analytical column was Hypersil ODS2 (250 mm x 4.6 mm, 5 microm), flow rate 1.0 mL x min(-1), injected volume 2 microL. The ionization source was ESI in positive ion mode.


RESULTS
Ferulic acid, nine known phthalides and one unknown phthalide derivative were tentatively identified in chromatograms based on their MS data and the comparison of their UV spectra with those published in the literatures.


CONCLUSIONS
The structural information of phthalides was obtained via HPLC-MS/MS, which provides an accurate and fast method to identify the phthalides and provides more scientific information for quality control of Danggui.
Yang
Fan
F
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Xiao
Yuan-sheng
YS
Zhang
Fei-fang
FF
Xue
Xing-ya
XY
Xu
Qing
Q
Liang
Xin-miao
XM
D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry; D029971:Angelica sinensis / Q000737:chemistry*; D001572:Benzofurans / Q000032:analysis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003373:Coumaric Acids / Q000032:analysis / Q000737:chemistry; D015394:Molecular Structure; D010796:Phthalic Anhydrides / Q000032:analysis / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D001572:Benzofurans; D003373:Coumaric Acids; D010796:Phthalic Anhydrides; C027820:ligustilide; C045855:3-N-butyl-4,5-dihydrophthalide; C004999:ferulic acid; D015107:4-Butyrolactone; C026105:butylidenephthalide