An interferon-gamma-producing Th1 subset is the major source of IL-17 in experimental autoimmune encephalitis.
ThIL-17 (IL-17+/IFN-gamma-) cell lines are significantly more encephalitogenic than Th1 (IL-17-/IFN-gamma+) cell lines in adoptive transfer EAE models. In actively induced EAE short ex vivo peptide stimulation identifies an IL-17+/IFN-gamma+ population of CD4+ CNS-infiltrating MOG35-55-specific T cells, which outnumber IL-17+/IFN-gamma- cells by approximately 3:1 as disease develops. A decrease in numbers of IL-17+/IFN-gamma+ cells following in vitro culture is accompanied by an increase in IL-17-/IFN-gamma+ cell numbers. Together these ex vivo and in vitro observations imply that the Th1 lineage is more encephalitogenic than is suggested by adoptive transfer of Th1 (IL-17-/IFN-gamma+) cell lines which have been terminally differentiated in vitro.
Suryani
Santi
S
The Garvan Institute of Medical Research, Department of Inflammation, 384 Victoria Street, Darlinghurst, Sydney, NSW 2010, Australia.
Sutton
Ian
I
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002490:Central Nervous System / Q000473:pathology; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000139:chemically induced / Q000473:pathology*; D005434:Flow Cytometry / Q000379:methods; D006023:Glycoproteins; D007371:Interferon-gamma / Q000494:pharmacology*; D020381:Interleukin-17 / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments; D018417:Th1 Cells / Q000187:drug effects* / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014316:Tritium / Q000378:metabolism
D016207:Cytokines; D006023:Glycoproteins; D020381:Interleukin-17; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments; C445081:myelin oligodendrocyte glycoprotein (35-55); D014316:Tritium; D007371:Interferon-gamma; D013936:Thymidine