Application of quantum dots to multicolor flow cytometry.
The coupling of quantum dots (QDs) to antibodies heralds a new era of versatility in multicolor flow cytometry. This chapter introduces the properties of QDs (as relevant to flow cytometric applications), the advantages derived from these properties, and the procedure for conjugating antibodies to QDs. Finally, we discuss strategies for choosing the combinations of QDs and antibodies best suited for multicolor experiments.
Chattopadhyay
Pratip K
PK
Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
Yu
Joanne
J
Roederer
Mario
M
D000906:Antibodies / Q000737:chemistry; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D045663:Quantum Dots; D015203:Reproducibility of Results
D000906:Antibodies; D005456:Fluorescent Dyes