Development of a semiquantitative degenerate real-time pcr-based assay for estimation of numbers of butyryl-coenzyme A (CoA) CoA transferase genes in complex bacterial samples.
We describe a new degenerate real-time PCR approach to simultaneously quantify phylogenetically different butyrate-producing bacteria based on the detection of butyryl-coenzyme A (CoA) CoA transferase genes. This pathway is present in numerically important groups of butyrate producers within the human colon, and thus this assay estimates the butyrate-producing ability of the microbiota.
Louis
Petra
P
Microbial Ecology Group, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, United Kingdom. p.louis@rowett.ac.uk
Flint
Harry J
HJ
D000595:Amino Acid Sequence; D001419:Bacteria / Q000201:enzymology*; D002087:Butyrates / Q000378:metabolism; D015256:Coenzyme A-Transferases / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics*; D005243:Feces / Q000382:microbiology; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D017386:Sequence Homology, Amino Acid
D002087:Butyrates; D017931:DNA Primers; D015256:Coenzyme A-Transferases