Protective effects of histidine dipeptides on the modification of neurofilament-L by the cytochrome c/hydrogen peroxide system.
Neurofilament-L (NF-L) is a major element of the neuronal cytoskeleton and is essential for neuronal survival. Moreover, abnormalities in NF-L result in neurodegenerative disorders. Carnosine and the related endogeneous histidine dipeptides prevent protein modifications such as oxidation and glycation. In the present study, we investigated whether histidine dipeptides, carnosine, homocarnosine, or anserine protect NF-L against oxidative modification during reaction between cytochrome c and H(2)O(2). Carnosine, homocarnosine and anserine all prevented cytochrome c/H(2)O(2)-mediated NF-L aggregation. In addition, these compounds also effectively inhibited the formation of dityrosine, and this inhibition was found to be associated with the reduced formations of oxidatively modified proteins. Our results suggest that carnosine and histidine dipeptides have antioxidant effects on brain proteins under pathophysiological conditions leading to degenerative damage, such as, those caused by neurodegenerative disorders.
Kim
Nam Hoon
NH
Department of Genetic Engineering, Cheongju University, Cheongju 360-764, Korea.
Kang
Jung Hoon
JH
D000818:Animals; D000861:Anserine / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D002336:Carnosine / Q000031:analogs & derivatives / Q000494:pharmacology; D045304:Cytochromes c / Q000502:physiology*; D004151:Dipeptides / Q000494:pharmacology*; D006639:Histidine / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D051379:Mice; D016900:Neurofilament Proteins / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D020011:Protective Agents / Q000494:pharmacology; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
D000975:Antioxidants; D004151:Dipeptides; D016900:Neurofilament Proteins; D020011:Protective Agents; C044882:neurofilament protein L; C005038:homocarnosine; D014443:Tyrosine; D006639:Histidine; D002336:Carnosine; D045304:Cytochromes c; D006861:Hydrogen Peroxide; C007543:dityrosine; D000861:Anserine