One-dimensional microfluidic beads array for multiple mRNAs expression detection.
A one-dimensional microfluidic beads array for in vitro rapid measurement of multiple mRNAs expression is presented in this paper. Gene specific capture DNA-functional beads were deposited along a microchannel to form an addressable beads array. We demonstrated that the one-dimensional beads array could perform simultaneous multiple nucleic acid targets detection and a DNA detection limit of 0.02 nM was obtained. Using this array, transcripts expression of three tumor-associated genes, including p53, H-ras, and NME1, both in CNE2 nasopharyngeal carcinoma cell lines and in normal human nasopharyngeal epithelial cells were evaluated. The responses of these three genes expression in CNE2 cells to 5-flouorouracil (5-Fu) stimuli were also assessed. Validation of these results was performed using reverse transcriptase-PCR. The presented methodology combines high throughput of microarrays and low sample consumption, convenient liquid handling of microfluidics. It enables rapid and facile determination of multi-gene expression and holds great potential in early cancer diagnostics and molecular biology.
Wen
Jianhui
J
State Key Laboratory of Chemo/Biosensing and Chemometrics, Biomedical Engineering Center, College of Chemistry and Chemical Engineering, Hunan University, Engineering Research Center for Bio-Nanotechnology of Hunan Province, Changsha, PR China.
Yang
Xiaohai
X
Wang
Kemin
K
Tan
Weihong
W
Zhou
Leiji
L
Zuo
Xinbing
X
Zhang
He
H
Chen
Yunqing
Y
D002478:Cells, Cultured; D004847:Epithelial Cells / Q000378:metabolism*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D020869:Gene Expression Profiling / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000295:instrumentation* / Q000379:methods; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics*
D012333:RNA, Messenger