title: Evidences for interaction of PsbS with photosynthetic complexes in maize thylakoids.
abstract: The PsbS subunit of Photosystem II (PSII) has received much attention in the past few years, given its crucial role in photoprotection of higher plants. The exact location of this small subunit in thylakoids is also debated. In this work possible interaction partners of PsbS have been identified by immunoaffinity and immunoprecipitation, performed with mildly solubilized whole thylakoid membrane. The interacting proteins, as identified by mass spectrometry analysis of the immunoaffinity eluate, include CP29, some LHCII components, but also components of Photosystem I, of the cytochrome b(6)f complex as well as of ATP synthase. These proteins can be co-immunoprecipitated by using highly specific anti-PsbS antibodies and, vice-versa, PsbS is co-immunoprecipitated by antisera against components of the interacting complexes. We also find that PsbS co-migrates with bands containing PSII, ATP synthase and cytochrome b(6)f as well as with LHCII-containing bands on non-denaturing Deriphat PAGE. These results suggest multiple location of PsbS in the thylakoid membrane and point to an unexpected lateral mobility of this PSII subunit. As revealed by immunogold labelling with antibody against PsbS, the protein is associated either with granal membranes or prevalently with stroma lamellae in low or high-intensity light-treated intact leaves, respectively. This finding is consistent with the capability of PsbS to interact with complexes located in stroma lamellae, even though the exact physiological condition(s) under which these interactions may take place remain to be clarified.
authors.affiliation: Department of Biology, University of Padova, Viale G. Colombo 3, 35131 Padua, Italy.
authors.forename: Enrico
authors.initials: E
authors.lastname: Teardo
authors.forename: Patrizia Polverino
authors.initials: PP
authors.lastname: de Laureto
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Bergantino
authors.forename: Francesca
authors.initials: F
authors.lastname: Dalla Vecchia
authors.forename: Fernanda
authors.initials: F
authors.lastname: Rigoni
authors.forename: Ildikò
authors.initials: I
authors.lastname: Szabò
authors.forename: Giorgio Mario
authors.initials: GM
authors.lastname: Giacometti
mesh_terms: D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism* / Q000648:ultrastructure; D045332:Photosystem II Protein Complex / Q000378:metabolism* / Q000648:ultrastructure; D010940:Plant Proteins / Q000378:metabolism* / Q000648:ultrastructure; D020524:Thylakoids / Q000378:metabolism* / Q000648:ultrastructure; D003313:Zea mays / Q000378:metabolism*
chemical_list: D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins