title: Viability of pathogenic Naegleria and Acanthamoeba isolates during 10 years of cryopreservation.
abstract: This is a follow-up report on the viability of pathogenic Naegleria fowleri, Naegleria australiensis and Acanthamoeba castellanii isolates during 5 to 10 years of cryopreservation at -70 degrees C. The greatest decrease in viability occurred with N. fowleri and the least occurred with N. australiensis. At 10 years of cryostorage, viability was 21% for N. fowleri, 32% for A. castellanii and 51% for N. australiensis.
authors.affiliation: Center for Health Sciences, Oklahoma State University, 1111 West 17th Street, Tulsa, Oklahoma 74107, USA. johndt@chs.okstate.edu
authors.forename: David T
authors.initials: DT
authors.lastname: John
authors.forename: Rebecca A
authors.initials: RA
authors.lastname: John
mesh_terms: D048248:Acanthamoeba castellanii; D000818:Animals; D015925:Cryopreservation; D016848:Naegleria fowleri; D013997:Time Factors