title: Amphiphilic effects of dibucaine HCl on rotational mobility of n-(9-anthroyloxy)stearic acid in neuronal and model membranes.
abstract: We studied dibucaine's effects on specific locations of n-(9-anthroyloxy)palmitic acid or stearic acid (n-AS) within phospholipids of synaptosomal plasma membrane vesicles isolated from bovine cerebral cortex (SPMV) and model membranes. Giant unilamellar vesicles (GUVs) were prepared with total lipids (SPMVTL) and mixture of several phospholipids (SPMVPL) extracted from SPMV. Dibucaine.HCl increased rotational mobility (increased disordering) of hydrocarbon interior, but it decreased mobility (increased ordering) of membrane interface, in both native and model membranes. The degree of rotational mobility in accordance with the carbon atom numbers of phospholipids comprising neuronal and model membranes was in the order at the 16, 12, 9, 6 and 2 position of aliphatic chain present in phospholipids. The sensitivity of increasing or decreasing effect of rotational mobility of hydrocarbon interior or surface region by dibucaine.HCl differed depending on the neuronal and model membranes in the descending order of SPMV, SPMVPL and SPMVTL.
authors.affiliation: Department of Dental Pharmacology and Biophysics, College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University, Busan 602-739, Republic of Korea.
authors.forename: Young-Ho
authors.initials: YH
authors.lastname: Lee
authors.forename: No-Soo
authors.initials: NS
authors.lastname: Park
authors.forename: Jong-Deok
authors.initials: JD
authors.lastname: Kwon
authors.forename: Ju-Seok
authors.initials: JS
authors.lastname: Park
authors.forename: Gye-Beom
authors.initials: GB
authors.lastname: Shin
authors.forename: Chung-Seob
authors.initials: CS
authors.lastname: Lee
authors.forename: Tae-Sang
authors.initials: TS
authors.lastname: Jung
authors.forename: Nak-Jin
authors.initials: NJ
authors.lastname: Choi
authors.forename: Jin-Hyeok
authors.initials: JH
authors.lastname: Yoon
authors.forename: Jin-Seok
authors.initials: JS
authors.lastname: Ok
authors.forename: Ung-Chan
authors.initials: UC
authors.lastname: Yoon
authors.forename: Moon-Kyung
authors.initials: MK
authors.lastname: Bae
authors.forename: Hye-Ock
authors.initials: HO
authors.lastname: Jang
authors.forename: Il
authors.initials: I
authors.lastname: Yun
mesh_terms: D000779:Anesthetics, Local / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D002540:Cerebral Cortex / Q000166:cytology; D003992:Dibucaine / Q000737:chemistry / Q000494:pharmacology*; D006838:Hydrocarbons / Q000737:chemistry; D009474:Neurons / Q000648:ultrastructure; D010743:Phospholipids / Q000737:chemistry; D013229:Stearic Acids / Q000737:chemistry*; D013500:Surface Tension; D013570:Synaptic Membranes / Q000737:chemistry* / Q000187:drug effects*
chemical_list: D000779:Anesthetics, Local; D006838:Hydrocarbons; D010743:Phospholipids; D013229:Stearic Acids; D003992:Dibucaine