title: The vanilloid receptor initiates and maintains colonic hypersensitivity induced by neonatal colon irritation in rats.
abstract: OBJECTIVE
  Robust chemical or mechanical irritation of the colon of neonatal rats leads to chronic visceral hypersensitivity. The clinical and physiologic relevance of such noxious stimulation in the context of human irritable bowel syndrome is questionable. The aims of this study were to determine whether mild chemical irritation of the colon of neonatal rats produced persistent changes in visceral sensitivity and to evaluate the role of transient receptor potential vanilloid 1 (TRPV1) in the initiation and maintenance of visceral hypersensitivity.
  
  
  METHODS
  Ten-day-old rat pups received an intracolonic infusion of 0.5% acetic acid in saline. TRPV1 inhibitors were administered 30 minutes before acetic acid sensitization. Sensitivity of the colon to balloon distention (CRD) in adults was measured by grading their abdominal withdrawal reflex and electromyographic responses. In adult rats, TRPV1 antagonist was injected intraperitoneally 30 minutes before CRD.
  
  
  RESULTS
  Neonatal acetic acid treatment resulted in higher sensitivity to CRD in adult rats compared with controls in the absence of histopathologic signs of inflammation. Treatment of colons of adult rats with acetic acid did not produce persistent sensitization. Antagonism of the TRPV1 before neonatal administration of acetic acid and after established visceral hypersensitivity attenuated sensitivity to CRD. TRPV1 expression was increased in dorsal root ganglia-containing colon afferent neurons.
  
  
  CONCLUSIONS
  We have described a new model for persistent colonic sensory dysfunction following a transient noxious stimulus in the neonatal period and a potentially important role for TRPV1 in initiation and maintenance of persistent visceral hypersensitivity.
authors.affiliation: Enteric Neuromuscular Disorders and Pain Laboratory, Division of Gastroenterology and Hepatology, Department of Internal Medicine, University of Texas Medical Branch, Galveston 77555-0764, USA.
authors.forename: John
authors.initials: J
authors.lastname: Winston
authors.forename: Mohan
authors.initials: M
authors.lastname: Shenoy
authors.forename: Dylan
authors.initials: D
authors.lastname: Medley
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Naniwadekar
authors.forename: Pankaj Jay
authors.initials: PJ
authors.lastname: Pasricha
mesh_terms: D019342:Acetic Acid; D000367:Age Factors; D000813:Anilides / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D002211:Capsaicin / Q000494:pharmacology; D002404:Catheterization; D002934:Cinnamates / Q000494:pharmacology; D003106:Colon / Q000187:drug effects / Q000294:innervation / Q000473:pathology / Q000503:physiopathology*; D004195:Disease Models, Animal; D004224:Diterpenes / Q000494:pharmacology; D004576:Electromyography; D005727:Ganglia, Spinal / Q000378:metabolism; D006930:Hyperalgesia / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology; D043183:Irritable Bowel Syndrome / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D008297:Male; D010146:Pain / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012020:Reflex, Abdominal / Q000187:drug effects; D050916:TRPV Cation Channels / Q000187:drug effects / Q000378:metabolism*; D017833:Visceral Afferents / Q000187:drug effects / Q000378:metabolism* / Q000503:physiopathology
chemical_list: D000813:Anilides; D002934:Cinnamates; D004224:Diterpenes; C477659:N-(3-methoxyphenyl)-4-chlorocinnamanilide; D050916:TRPV Cation Channels; C493922:Trpv1 protein, rat; C468130:iodoresiniferatoxin; D019342:Acetic Acid; D002211:Capsaicin