[Preparation and identification of dengue virus with deletion mutation of capsid protein].
OBJECTIVE
To generate rescued viruses with deletion mutation of capsid protein from dengue virus type 2 isolated in China (DEN2-43).


METHODS
On the basis of infectious full-length cDNA clone pD212 of DEN2-43 strain virus, the deletion mutants were constructed by fusion PCR, from which the rescued viruses with deletion mutation of capsid protein were generated by transcription in vitro and electroporation.


CONCLUSIONS
Sequence analysis demonstrated that the deletion mutations had been successfully inserted into the rescued viruses obtained. These mutant viruses may hold the key for elucidating the effects of deletion mutation of capsid protein on the biological characteristics of dengue virus.
Zhu
Wu-Yang
WY
State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.
Qin
E-de
ED
Yu
Man
M
Qin
Cheng-Feng
CF
Yu
Xue-Dong
XD
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D003716:Dengue Virus / Q000235:genetics* / Q000302:isolation & purification; D018274:Electroporation; D008969:Molecular Sequence Data; D017384:Sequence Deletion; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014779:Virus Replication / Q000235:genetics
D036022:Capsid Proteins