title: Molecular cloning of tropomyosins identified as allergens in six species of crustaceans.
abstract: Although tropomyosin is known to be a major allergen of crustaceans, its structural information is limited to only five species. In this study, tropomyosin was confirmed to be a major allergen in six species of crustaceans (black tiger prawn, kuruma prawn, pink shrimp, king crab, snow crab, and horsehair crab) by immunoblotting. Then, the amino acid sequences of tropomyosins from these crustaceans were elucidated by a cDNA cloning technique. Sequence data for crustacean tropomyosins including the obtained results reveal that fast tropomyosins are contained in shrimps (or prawns) and lobsters, slow tropomyosins in crabs, and both tropomyosins in crayfishes and hermit crabs. Although fast and slow tropomyosins share a high sequence identity (about 90%) with each other, significant differences are observed in specific regions between both tropomyosins.
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: Kanna
authors.initials: K
authors.lastname: Motoyama
authors.forename: Yota
authors.initials: Y
authors.lastname: Suma
authors.forename: Shoichiro
authors.initials: S
authors.lastname: Ishizaki
authors.forename: Yuji
authors.initials: Y
authors.lastname: Nagashima
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shiomi
mesh_terms: D000485:Allergens / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003445:Crustacea / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D018076:DNA, Complementary / Q000737:chemistry; D005512:Food Hypersensitivity / Q000097:blood; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D014335:Tropomyosin / Q000737:chemistry / Q000235:genetics* / Q000276:immunology
chemical_list: D000485:Allergens; D018076:DNA, Complementary; D014335:Tropomyosin