Surface modification of a porous polyurethane through a culture of human osteoblasts and an electromagnetic bioreactor.
There is increasing interest in new biomaterials and new culture methods for bone tissue engineering, in order to produce, in vitro, living constructs able to integrate in the surrounding tissue. Using an electromagnetic bioreactor (magnetic field intensity, 2 mT; frequency, 75 Hz), we investigated the effects of electromagnetic stimulation on SAOS-2 human osteoblasts seeded onto a porous polyurethane. In comparison with control conditions, the electromagnetic stimulation caused higher cell proliferation, increased surface coating with decorin and type-I collagen, and higher calcium deposition. The immunolocalization of decorin and type-I collagen showed their colocalization in the cell-rich areas. The use of an electromagnetic bioreactor aimed at obtaining the surface modification of the porous polyurethane in terms of cell colonization and coating with calcified matrix. The superficially modified biomaterial could be used, in clinical applications, as an implant for bone repair.
Fassina
L
L
Dipartimento di Informatica e Sistemistica, University of Pavia, Pavia, Italy. lorenzo.fassina@unipv.it
Visai
L
L
De Angelis
M G Cusella
MG
Benazzo
F
F
Magenes
G
G
D019149:Bioreactors; D002118:Calcium; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D024042:Collagen Type I / Q000737:chemistry; D058575:Decorin; D004558:Electric Stimulation; D004574:Electromagnetic Fields; D005109:Extracellular Matrix; D016326:Extracellular Matrix Proteins; D006801:Humans; D007150:Immunohistochemistry; D010006:Osteoblasts / Q000737:chemistry*; D011140:Polyurethanes; D011509:Proteoglycans; D023822:Tissue Engineering / Q000379:methods*
D024042:Collagen Type I; C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; D011140:Polyurethanes; D011509:Proteoglycans; D002118:Calcium