title: Diverse and potent chemokine production by lung CD11bhigh dendritic cells in homeostasis and in allergic lung inflammation.
abstract: Lung CD11c(high) dendritic cells (DC) are comprised of two major phenotypically distinct populations, the CD11b(high) DC and the integrin alpha(E)beta(7)(+) DC (CD103(+) DC). To examine whether they are functionally distinguishable, global microarray studies and real-time PCR analysis were performed. Significant differences between the two major CD11c(high) DC types in chemokine mRNA expression were found. CD11b(high) DC is a major secretory cell type and highly expressed at least 16 chemokine mRNA in the homeostatic state, whereas CD103(+) DC highly expressed only 6. Intracellular chemokine staining of CD11c(high) lung cells including macrophages, and ELISA determination of sort-purified CD11c(high) cell culture supernatants, further showed that CD11b(high) DC produced the highest levels of 9 of 14 and 5 of 7 chemokines studied, respectively. Upon LPS stimulation in vitro and in vivo, CD11b(high) DC remained the highest producer of 7 of 10 of the most highly produced chemokines. Induction of airway hyperreactivity and lung inflammation increased lung CD11b(high) DC numbers markedly, and they produced comparable or higher amounts of 11 of 12 major chemokines when compared with macrophages. Although not a major producer, CD103(+) DC produced the highest amounts of the Th2-stimulating chemokines CCL17/thymus and activation-related chemokine and CCL22/monocyte-derived chemokine in both homeostasis and inflammation. Significantly, CCL22/monocyte-derived chemokine exhibited regulatory effects on CD4(+) T cell proliferation. Further functional analysis showed that both DC types induced comparable Th subset development. These studies showed that lung CD11b(high) DC is one of the most important leukocyte types in chemokine production and it is readily distinguishable from CD103(+) DC in this secretory function.
authors.affiliation: Department of Internal Medicine, University of Virginia Health Sciences Center, Charlottesville, VA 22908, USA.
authors.forename: Steven R
authors.initials: SR
authors.lastname: Beaty
authors.forename: C Edward
authors.initials: CE
authors.lastname: Rose
authors.forename: Sun-Sang J
authors.initials: SS
authors.lastname: Sung
mesh_terms: D000818:Animals; D015703:Antigens, CD; D018845:CD11 Antigens; D015496:CD4-Positive T-Lymphocytes; D049109:Cell Proliferation; D018925:Chemokines / Q000096:biosynthesis* / Q000235:genetics; D003713:Dendritic Cells / Q000276:immunology*; D020869:Gene Expression Profiling; D006706:Homeostasis; D006967:Hypersensitivity / Q000276:immunology / Q000473:pathology*; D007249:Inflammation / Q000276:immunology*; D039001:Integrin alpha Chains; D008171:Lung Diseases / Q000276:immunology* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000032:analysis; D018418:Th2 Cells
chemical_list: D015703:Antigens, CD; D018845:CD11 Antigens; D018925:Chemokines; D039001:Integrin alpha Chains; D012333:RNA, Messenger; C091367:alpha E integrins