Effect of FPS1 deletion on the fermentation properties of Saccharomyces cerevisiae.
OBJECTIVE
To investigate the effect of FPS1 deletion on the fermentation properties of Saccharomyces cerevisiae and to evaluate whether FPS1 deletion would result in higher ethanol yield.


RESULTS
FPS1 of S. cerevisiae was knocked out using the one-step gene replacement method. The fermentation properties of the fps1Delta mutant under microaerobic conditions were investigated and compared with that of the wild type. Consumption of glucose, yield of ethanol, yield of glycerol, acetic acid and pyruvic acid were monitored. Compared with the wild type, the ethanol yield of the fps1Delta mutant was improved by 10 +/- 2% and glycerol yield decreased by 18.8 +/- 2%. Meanwhile, acetic acid yield decreased by 5.4 +/- 1%, and pyruvic acid yield decreased by 58.6 +/- 1%.


CONCLUSIONS
FPS1 deletion of S. cerevisiae resulted in reduced glycerol yield and higher ethanol yield.


CONCLUSIONS
The cost of carbon source in ethanol fermentation is an important factor in determining ethanol production. Approximately 5% carbon source is converted into glycerol in ethanol fermentation. Eliminating formation of glycerol through FPS1 deletion can be used to increase ethanol yield of S. cerevisiae without increasing the overall cost of carbon source.
Zhang
A
A
School of Chemical Engineering and Technology, Tianjin University, Weijing Road 92, Tianjin, China.
Kong
Q
Q
Cao
L
L
Chen
X
X
D005285:Fermentation; D005990:Glycerol / Q000378:metabolism; D008565:Membrane Proteins / Q000502:physiology*; D009243:NAD / Q000378:metabolism; D019289:Pyruvic Acid / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000502:physiology*
C093263:FPS1 protein, S cerevisiae; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; D009243:NAD; D019289:Pyruvic Acid; D005990:Glycerol