title: PtdIns(3,4,5)P(3)-dependent and -independent roles for PTEN in the control of cell migration.
abstract: BACKGROUND
  Phosphatase and tensin homolog (PTEN) mediates many of its effects on proliferation, growth, survival, and migration through its PtdIns(3,4,5)P(3) lipid phosphatase activity, suppressing phosphoinositide 3-kinase (PI3K)-dependent signaling pathways. PTEN also possesses a protein phosphatase activity, the role of which is less well characterized.
  
  
  RESULTS
  We have investigated the role of PTEN in the control of cell migration of mesoderm cells ingressing through the primitive streak in the chick embryo. Overexpression of PTEN strongly inhibits the epithelial-to-mesenchymal transition (EMT) of mesoderm cells ingressing through the anterior and middle primitive streak, but it does not affect EMT of cells located in the posterior streak. The inhibitory activity on EMT is completely dependent on targeting PTEN through its C-terminal PDZ binding site, but can be achieved by a PTEN mutant (PTEN G129E) with only protein phosphatase activity. Expression either of PTEN lacking the PDZ binding site or of the PTEN C2 domain, or inhibition of PI3K through specific inhibitors, does not inhibit EMT, but results in a loss of both cell polarity and directional migration of mesoderm cells. The PTEN-related protein TPTE, which normally lacks any detectable lipid and protein phosphatase activity, can be reactivated through mutation, and only this reactivated mutant leads to nondirectional migration of these cells in vivo.
  
  
  CONCLUSIONS
  PTEN modulates cell migration of mesoderm cells in the chick embryo through at least two distinct mechanisms: controlling EMT, which involves its protein phosphatase activity; and controlling the directional motility of mesoderm cells, through its lipid phosphatase activity.
authors.affiliation: Division of Molecular Physiology, University of Dundee, Dundee, DD1 5EH, Scotland, United Kingdom. n.r.leslie@dundee.ac.uk
authors.forename: Nick R
authors.initials: NR
authors.lastname: Leslie
authors.forename: Xuesong
authors.initials: X
authors.lastname: Yang
authors.forename: C Peter
authors.initials: CP
authors.lastname: Downes
authors.forename: Cornelis J
authors.initials: CJ
authors.lastname: Weijer
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D002642:Chick Embryo; D005775:Gastrula / Q000201:enzymology*; D008648:Mesoderm / Q000201:enzymology*; D051059:PTEN Phosphohydrolase / Q000378:metabolism*; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism*; D017434:Protein Structure, Tertiary
chemical_list: D018129:Phosphatidylinositol Phosphates; D051059:PTEN Phosphohydrolase