Isolation and characterization of Dichelobacter nodosus from ovine and caprine footrot in Kashmir, India.
Footrot is a highly contagious and economically important disease of sheep and goats, caused by Dichelobacter nodosus, a slow growing anaerobic Gram-negative rod. The current Australian antigenic classification system, based on variation in the fimbriae, classifies D. nodosus into at least 10 serogroups (A-I and M) and 18 serotypes. This investigation was intended to determine the serological diversity of D. nodosus in this region of Kashmir, India. Exudates of footrot lesions were collected from 24 naturally infected sheep and 42 goats located in the Kashmir valley. Of these 66 samples, 24 yielded evidence of D. nodosus by PCR using 16SrDNA specific primers. Multiplex PCR using serogroup specific primers revealed the presence of serogroup B in all the samples except two, which showed the presence of serogroup E D. nodosus. This study also documents the isolation of D. nodosus and detection of serogroup E for the first time in India.
Wani
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Bacteriology Laboratory, Division of Microbiology & Immunology, Faculty of Veterinary Sciences & A.H., S.K. University of Agricultural Sciences and Technology of Kashmir, Shuhama (Alusteng), Srinagar 190006, India. microlab@sancharnet.in
Samanta
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I
Kawoosa
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D000818:Animals; D020376:Dichelobacter nodosus / Q000302:isolation & purification*; D005535:Foot Rot / Q000382:microbiology*; D015511:Goat Diseases / Q000453:epidemiology / Q000382:microbiology*; D006041:Goats / Q000382:microbiology; D016905:Gram-Negative Bacterial Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D007194:India / Q000453:epidemiology; D012336:RNA, Ribosomal, 16S; D012756:Sheep / Q000382:microbiology; D012757:Sheep Diseases / Q000453:epidemiology / Q000382:microbiology*
D012336:RNA, Ribosomal, 16S