[Determination of main flavone glycosides in Flos Chrysanthemi and observation of factors influenced contents].
OBJECTIVE
To determine and compare the content of luteolin-7-O-beta-D-glucoside and apigenin-7-O-beta-D-glucoside in Flos Chrysanthemi from different collection time, sources, grades and processes.


METHODS
The contents were determined by RP-HPLC. Zorbax SB C18 column (4.6 mm x 250 mm, 5 microm) was used as analysis column, the mobile phase was acetonitrile-pH 2.0 phosphate buffer solution with gradient elution, the detector was set at 338 nm.


RESULTS
The contents of two components changed at some degree in Flos Chrysanthemi from different collection time, different plant sites or with different grades, while the contents varied obviously among Flos Chrysanthemi from different source and different sorts. No obvious difference was found in Flos Chrysanthemi from different year.


CONCLUSIONS
The contents of two components were influenced by process, plane site, source and sorts, especially by source and sorts.
Hu
Bi-Bo
BB
Department of Pharmaceutical Analysis and Drug Metabolism, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
Wu
Zu-Shuai
ZS
Cai
Jun
J
Jiang
Hui-Di
HD
D047310:Apigenin / Q000032:analysis*; D002681:China; D031202:Chrysanthemum / Q000737:chemistry*; D004339:Drug Compounding; D017753:Ecosystem; D035264:Flowers / Q000737:chemistry; D005960:Glucosides / Q000032:analysis*; D047311:Luteolin / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D012621:Seasons
D005960:Glucosides; C057792:apigetrin; D047310:Apigenin; C066408:luteolin-7-glucoside; D047311:Luteolin