Kinetics and organ distribution of IL-17-producing CD4 cells in proteolipid protein 139-151 peptide-induced experimental autoimmune encephalomyelitis of SJL mice.
In experimental autoimmune encephalomyelitis (EAE), the production of proinflammatory cytokines by neuroantigen-specific T cells is thought to initiate and maintain the inflammatory autoimmune pathology. Because gene knockout strategies have shown that IFN-gamma and TNF are not essential for EAE development, there is increasing interest in establishing the role of other proinflammatory cytokines, primarily IL-17 in EAE. We used an IL-17 ELISPOT assay to track the neuroantigen-specific IL-17-producing T cells at single-cell resolution in various organs of SJL mice undergoing PLP 139-151-induced EAE. Overall, the migration patterns and population kinetics of the PLP 139-151-specific IL-17-producing CD4 cells were reminiscent of the IFN-gamma-producing cells, with the exception of IL-17 producers far outnumbering the IFN-gamma and IL-2 producers in the inflamed CNS. The selective enrichment of IL-17-producing CD4 cells in the CNS is suggestive of the pathogenic role of an independent (non-Th1) IL-17-producing proinflammatory effector T cell class in EAE.
Hofstetter
Harald H
HH
Department of Pathology, Case Western Reserve University, Cleveland, OH 44106, USA.
Toyka
Klaus V
KV
Tary-Lehmann
Magdalena
M
Lehmann
Paul V
PV
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology*; D002465:Cell Movement; D002490:Central Nervous System / Q000166:cytology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000139:chemically induced / Q000276:immunology*; D007249:Inflammation / Q000209:etiology; D007371:Interferon-gamma; D020381:Interleukin-17 / Q000032:analysis*; D007376:Interleukin-2; D007700:Kinetics; D051379:Mice; D008815:Mice, Inbred Strains; D018991:Myelin Proteolipid Protein / Q000009:adverse effects; D009928:Organ Specificity; D010446:Peptide Fragments / Q000009:adverse effects; D006377:T-Lymphocytes, Helper-Inducer
D020381:Interleukin-17; D007376:Interleukin-2; D018991:Myelin Proteolipid Protein; D010446:Peptide Fragments; C435023:myelin proteolipid protein (139-151); D007371:Interferon-gamma