title: Minimum inhibitory concentration of adherence of Punica granatum Linn (pomegranate) gel against S. mutans, S. mitis and C. albicans.
abstract: The purpose of this study was to investigate the antimicrobial effect of a Punica granatum Linn (pomegranate) phytotherapeutic gel and miconazole (Daktarin oral gel) against three standard streptococci strains (mutans ATCC 25175, sanguis ATCC 10577 and mitis ATCC 9811), S. mutans clinically isolated and Candida albicans either alone or in association. The effect of minimum inhibitory concentrations of the gels on the adherence of these microorganisms to glass was assessed in the presence of 5% sucrose, using increasing and doubled concentrations of the diluted solution of the gels ranging from 1:1 to 1:1024. The minimum inhibitory concentrations of adherence of Punica granatum L. gel against the test organisms were: 1:16 for S. mutans (ATCC), S. mutans (CI) and S. sanguis; 1:128 for S. mitis and 1:64 for C. albicans. The minimum inhibitory concentrations of adherence of miconazole against the same organisms were: 1:512, 1:64, 1:4, 1:128 and 1:16, respectively. In experiments with three and four associated microorganisms, the Punica granatum L. gel had greater efficiency in inhibiting microbial adherence than the miconazole. The results of this study suggest that this phytotherapeutic agent might be used in the control of adherence of different microorganisms in the oral cavity.
authors.affiliation: Departament of Clinical and Social Dentistry, Federal University of Paraíba, João Pessoa, PB, Brazil. laurylene@uol.ocm.br
authors.forename: Laurylene César de Souza
authors.initials: LC
authors.lastname: Vasconcelos
authors.forename: Fábio Correia
authors.initials: FC
authors.lastname: Sampaio
authors.forename: Maria Carmélia Correia
authors.initials: MC
authors.lastname: Sampaio
authors.forename: Maria do Socorro Vieira
authors.initials: Mdo S
authors.lastname: Pereira
authors.forename: Jane Sheila
authors.initials: JS
authors.lastname: Higino
authors.forename: Maria Helena Pereira
authors.initials: MH
authors.lastname: Peixoto
mesh_terms: D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D000935:Antifungal Agents / Q000008:administration & dosage / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects; D002176:Candida albicans / Q000187:drug effects*; D005782:Gels; D006801:Humans; D029561:Lythraceae; D008825:Miconazole / Q000008:administration & dosage / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D008517:Phytotherapy; D028321:Plant Preparations / Q000008:administration & dosage / Q000494:pharmacology*; D034361:Streptococcus mitis / Q000187:drug effects*; D013295:Streptococcus mutans / Q000187:drug effects*; D013298:Streptococcus sanguis / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D000935:Antifungal Agents; D005782:Gels; D028321:Plant Preparations; D008825:Miconazole