title: Comparison of human protein-protein interaction maps.
abstract: BACKGROUND
  Large-scale mappings of protein-protein interactions have started to give us new views of the complex molecular mechanisms inside a cell. After initial projects to systematically map protein interactions in model organisms such as yeast, worm and fly, researchers have begun to focus on the mapping of the human interactome. To tackle this enormous challenge, different approaches have been proposed and pursued. While several large-scale human protein interaction maps have recently been published, their quality remains to be critically assessed.
  
  
  RESULTS
  We present here a first comparative analysis of eight currently available large-scale maps with a total of over 10,000 unique proteins and 57,000 interactions included. They are based either on literature search, orthology or by yeast-two-hybrid assays. Comparison reveals only a small, but statistically significant overlap. More importantly, our analysis gives clear indications that all interaction maps imply considerable selection and detection biases. These results have to be taken into account for future assembly of the human interactome.
  
  
  BACKGROUND
  An integrated human interaction network called Unified Human Interactome (UniHI) is made publicly accessible at http://www.mdc-berlin.de/unihi.
  
  
  BACKGROUND
  Supplementary data are available at Bioinformatics online.
authors.affiliation: Institute for Theoretical Biology, Charité, Humboldt-Universität, 10115 Berlin, Germany. m.futschik@biologie.hu-berlin.de
authors.forename: Matthias E
authors.initials: ME
authors.lastname: Futschik
authors.forename: Gautam
authors.initials: G
authors.lastname: Chaurasia
authors.forename: Hanspeter
authors.initials: H
authors.lastname: Herzel
mesh_terms: D019295:Computational Biology; D030562:Databases, Protein; D006801:Humans; D025941:Protein Interaction Mapping; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics
chemical_list: D011506:Proteins