title: Increased midkine gene expression in childhood B-precursor acute lymphoblastic leukemia.
abstract: Midkine (MK) is a heparin-binding growth factor that is overexpressed in a number of solid cancers. However, expression in acute leukemia has not been clarified. We examined MK gene expression using real-time PCR in 94 children with acute leukemia. In 30 of the 41 patients with B-precursor ALL, MK gene expression was overexpressed than normal BM. MK gene was also overexpressed in more than half of patients with FAB M1 and M2 types of AML. Quantification of MK gene by real-time PCR offers particular promise as a prognostic marker and a marker for minimal residual disease in children with B-precursor ALL.
authors.affiliation: Department of Pediatrics, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Hidaka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yagasaki
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Asahito
authors.initials: A
authors.lastname: Hama
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Nishio
authors.forename: Makito
authors.initials: M
authors.lastname: Tanaka
authors.forename: Nao
authors.initials: N
authors.lastname: Yoshida
authors.forename: Itzel Bustos
authors.initials: IB
authors.lastname: Villalobos
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.forename: Yinyan
authors.initials: Y
authors.lastname: Xu
authors.forename: Keizo
authors.initials: K
authors.lastname: Horibe
authors.forename: Sen
authors.initials: S
authors.lastname: Chen
authors.forename: Kenji
authors.initials: K
authors.lastname: Kadomatsu
authors.forename: Seiji
authors.initials: S
authors.lastname: Kojima
mesh_terms: D000293:Adolescent; D015153:Blotting, Western; D001853:Bone Marrow / Q000378:metabolism / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D005260:Female; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D015448:Leukemia, B-Cell / Q000235:genetics* / Q000378:metabolism; D007951:Leukemia, Myeloid / Q000235:genetics* / Q000378:metabolism; D008297:Male; D000078792:Midkine; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D009414:Nerve Growth Factors / Q000235:genetics* / Q000378:metabolism; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C066254:MDK protein, human; D009363:Neoplasm Proteins; D009414:Nerve Growth Factors; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D000078792:Midkine