Testis-specific expression of the beta2 tubulin promoter of Aedes aegypti and its application as a genetic sex-separation marker.
Sex-specific expression of transgenes in pest insects enables novel genetic control strategies, based either on genetic sexing or the spread of transgenes through the germ-line, to be developed and then tested for implementation. We describe the isolation of the beta tubulin genes from the yellow fever mosquito, Aedes aegypti, and the identification of the particular beta2 tubulin gene which has expression confined to the testes. We demonstrate that the beta2 tubulin promoter of Ae. aegypti can direct the expression of a DsRed genetic marker in the testes and show that labelled sperm can be detected in inseminated spermathecae. The applications for this technology in the genetic control of Ae. aegypti are discussed.
Smith
R C
RC
Program in Cell, Molecular, and Developmental Biology, University of California, Riverside, CA 92521, USA.
Walter
M F
MF
Hice
R H
RH
O'Brochta
D A
DA
Atkinson
P W
PW
D000330:Aedes / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D005819:Genetic Markers / Q000235:genetics; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D030781:Organisms, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012727:Sex Characteristics; D013737:Testis / Q000378:metabolism*; D014404:Tubulin / Q000235:genetics*
D005819:Genetic Markers; D019476:Insect Proteins; D008164:Luminescent Proteins; D014404:Tubulin