title: Alignment-based approach for durable data storage into living organisms.
abstract: The practical realization of DNA data storage is a major scientific goal. Here we introduce a simple, flexible, and robust data storage and retrieval method based on sequence alignment of the genomic DNA of living organisms. Duplicated data encoded by different oligonucleotide sequences was inserted redundantly into multiple loci of the Bacillus subtilis genome. Multiple alignment of the bit data sequences decoded by B. subtilis genome sequences enabled the retrieval of stable and compact data without the need for template DNA, parity checks, or error-correcting algorithms. Combined with the computational simulation of data retrieval from mutated message DNA, a practical use of this alignment-based method is discussed.
authors.affiliation: Institute for Advanced Biosciences, Keio University, 403-1 Nipponkoku, Daihoji, Tsuruoka, 997-0017 Yamagata, Japan.
authors.forename: Nozomu
authors.initials: N
authors.lastname: Yachie
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Sekiyama
authors.forename: Junichi
authors.initials: J
authors.lastname: Sugahara
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Ohashi
authors.forename: Masaru
authors.initials: M
authors.lastname: Tomita
mesh_terms: D001483:Base Sequence; D003198:Computer Simulation; D039301:Computers, Molecular; D004252:DNA Mutational Analysis / Q000379:methods; D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics*; D005815:Genetic Code; D016680:Genome, Bacterial / Q000235:genetics; D016247:Information Storage and Retrieval / Q000379:methods*; D008956:Models, Chemical; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016415:Sequence Alignment / Q000379:methods*; D012815:Signal Processing, Computer-Assisted
chemical_list: D004269:DNA, Bacterial