title: Bacterial infection promotes DNA hypermethylation.
abstract: Maternal oral infection, caused by bacteria such as C. rectus or P. gingivalis, has been implicated as a potential source of placental and fetal infection and inflammatory challenge, which increases the relative risk for pre-term delivery and growth restriction. Intra-uterine growth restriction has also been reported in various animal models infected with oral organisms. Analyzing placental tissues of infected growth-restricted mice, we found down-regulation of the imprinted Igf2 gene. Epigenetic modification of imprinted genes via changes in DNA methylation plays a critical role in fetal growth and development programming. Here, we assessed whether C. rectus infection mediates changes in the murine placenta Igf2 methylation patterns. We found that infection induced hypermethylation in the promoter region-P0 of the Igf2 gene. This novel finding, correlating infection with epigenetic alterations, provides a mechanism linking environmental signals to placental phenotype, with consequences for development.
authors.affiliation: University of North Carolina at Chapel Hill, Center for Oral and Systemic Diseases, Department of Periodontology, UNC School of Dentistry, CB #7455, DRC Rm 222, Chapel Hill, NC 27599-7455, USA.
authors.forename: Y A
authors.initials: YA
authors.lastname: Bobetsis
authors.forename: S P
authors.initials: SP
authors.lastname: Barros
authors.forename: D M
authors.initials: DM
authors.lastname: Lin
authors.forename: J R
authors.initials: JR
authors.lastname: Weidman
authors.forename: D C
authors.initials: DC
authors.lastname: Dolinoy
authors.forename: R L
authors.initials: RL
authors.lastname: Jirtle
authors.forename: K A
authors.initials: KA
authors.lastname: Boggess
authors.forename: J D
authors.initials: JD
authors.lastname: Beck
authors.forename: S
authors.initials: S
authors.lastname: Offenbacher
mesh_terms: D000818:Animals; D002169:Campylobacter Infections / Q000150:complications*; D044883:Campylobacter rectus; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D005260:Female; D005317:Fetal Growth Retardation / Q000209:etiology*; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D010920:Placenta / Q000378:metabolism / Q000473:pathology; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000235:genetics*; D011401:Promoter Regions, Genetic
chemical_list: C513121:IGF2 protein, mouse; D007335:Insulin-Like Growth Factor II