Resealing of electroporation of porcine epidermis using phospholipids and poloxamers.
The resealing of porcine epidermis after electroporation is investigated. Porcine epidermis was subjected to electroporation (30 pulses at 100 V, 1 ms and at 1 Hz) in a vertical diffusion apparatus, in the presence of 2 mg/ml dimyristoylphosphatidylserine, to produce a long lasting permeable state. Resealing treatments include incubation in 0.0625-0.25 mM poloxamer 188 (P188), or incorporation of phosphatidylcholines (PC) and/or cationic lipids with additional pulses. The recovery of electric resistance of the epidermis samples after electroporation with or without resealing treatments was monitored. The transports of carboxyfluorescein and glucose were measured during the recovery process. Both P188 and PC were effective in resealing in terms of electric conductance and transport, with P188 reacting more rapidly and completely. P188 mediated lipid exchange between stratum corneum lipid particles was measured by fluorescence resonance energy transfer (FRET). Lipid reorganization facilitated by P188 and PC is suggested to be a major resealing mechanism of electroporation damage.
Burgess
Sarah E
SE
Cancer Biology Department, Roswell Park Cancer Institute, Elm & Carlton Streets, Buffalo, NY 14263, USA.
Zhao
Yali
Y
Sen
Arindam
A
Hui
Sek Wen
SW
D000279:Administration, Cutaneous; D000818:Animals; D001692:Biological Transport; D002412:Cations / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004553:Electric Conductivity; D018274:Electroporation; D004817:Epidermis / Q000187:drug effects / Q000378:metabolism*; D005452:Fluoresceins / Q000493:pharmacokinetics; D031541:Fluorescence Resonance Energy Transfer; D005712:Galvanic Skin Response / Q000187:drug effects; D005947:Glucose / Q000493:pharmacokinetics; D008055:Lipids / Q000494:pharmacology*; D010539:Permeability; D010713:Phosphatidylcholines / Q000494:pharmacology*; D010718:Phosphatidylserines / Q000494:pharmacology; D020442:Poloxamer / Q000494:pharmacology*; D013552:Swine
D002412:Cations; D005452:Fluoresceins; D008055:Lipids; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; D020442:Poloxamer; C024098:6-carboxyfluorescein; C042859:dimyristoylphosphatidylserine; D005947:Glucose