title: Immunohistolocalization and gene expression of the secretory carbonic anhydrase isozymes (CA-VI) in canine oral mucosa, salivary glands and oesophagus.
abstract: The immunohistolocalization of secretory carbonic anhydrase isoenzymes (CA-VI) in canine salivary glands, parotid, submandibular, sublingual and zygomatic glands, oral and oesophageal mucosa was studied using a specific antiserum against a canine CA-VI. In addition, the gene expression of CA-VI from the same tissue was studied using a real-time reverse-transcriptase polymerase chain reaction. In all salivary glands and oesophageal gland, immunostaining intensely localized CA-VI antiserum throughout the cytoplasm of serous acinar cells, including serous demilune and ductal epithelial cells. In contrast, no immunoreaction localized CA-VI in the mucous acinar cells of the gland. CA-VI gene transcripts were also detected in the same areas. The physiological significance of secretory CA-VI in the oral and oesophageal cavity is thought to play a highly specialized role in the maintenance of bicarbonate level in saliva and to protect mucosa from acid injury. It is shown that the major sites of the CA-VI secretion in dogs were in serous (demilune) secretory cells in all four major salivary glands and oesophageal glands in particular.
authors.affiliation: Laboratory of Anatomy I, Azabu University School of Veterinary Medicine, Fuchinobe 1-17-71, Sagamihara, Kanagawa, 229-8501, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kasuya
authors.forename: S
authors.initials: S
authors.lastname: Shibata
authors.forename: M
authors.initials: M
authors.lastname: Kaseda
authors.forename: N
authors.initials: N
authors.lastname: Ichihara
authors.forename: T
authors.initials: T
authors.lastname: Nishita
authors.forename: M
authors.initials: M
authors.lastname: Murakami
authors.forename: M
authors.initials: M
authors.lastname: Asari
mesh_terms: D000818:Animals; D002256:Carbonic Anhydrases / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D004285:Dogs; D004947:Esophagus / Q000201:enzymology*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D007150:Immunohistochemistry / Q000379:methods / Q000662:veterinary*; D008297:Male; D009061:Mouth Mucosa / Q000201:enzymology*; D021122:Protein Subunits / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D012469:Salivary Glands / Q000201:enzymology*
chemical_list: D021122:Protein Subunits; D002256:Carbonic Anhydrases; C477051:carbonic anhydrase VI