Interleukin-18 and its three gene polymorphisms relating to allergic rhinitis.
The study aimed to examine an association of three different single nucleotide polymorphisms (SNPs) of the IL-18 gene (-607 C/A, -137 G/C and -133 C/G) on chromosome 11q22 with allergic rhinitis (AR). Genotyping for the SNPs was performed using 539 patients with AR and 312 healthy control volunteers. Positivity to the skin prick test for the fungus Alternaria sp. in patients with AR, and IgE levels according to particular genotypes of selected SNPs, were also determined. There were no significant differences in the distribution of single IL-18 alleles or genotypes between controls and AR patients. However, frequencies of combined IL-18 genotypes arising from combinations of the three common polymorphisms (-607, -137 and -133) were significantly different between both groups (P = 0.009, P (corr) < 0.05, OR = 5.35, 95% CI: 1.9-15.2). There was a marginally significant association of the IL-18-607 variant with IgE levels (P = 0.05) in patients, but not in the case of the other SNPs. Patients allergic to Alternaria, but not those allergic to other antigens, showed a significant association with the IL-18-607 polymorphism (P = 0.0037, P (corr) < 0.05). Results suggest that IL-18 gene variants may be one of the factors participating in the pathogenesis of AR or its intermediary phenotypes.
Sebelova
Sarka
S
Department of Pathophysiology, Faculty of Medicine, Masaryk University, Komenského nám. 2, 662 43, Brno, Czech Republic. ssebelova@chello.cz.
Izakovicova-Holla
Lydie
L
Department of Pathophysiology, Faculty of Medicine, Masaryk University, Komenského nám. 2, 662 43, Brno, Czech Republic.
Stejskalova
Andrea
A
Department of Pathophysiology, Faculty of Medicine, Masaryk University, Komenského nám. 2, 662 43, Brno, Czech Republic.
Schüller
Marcel
M
Department of Pathophysiology, Faculty of Medicine, Masaryk University, Komenského nám. 2, 662 43, Brno, Czech Republic.
Znojil
Vladimir
V
Department of Pathophysiology, Faculty of Medicine, Masaryk University, Komenského nám. 2, 662 43, Brno, Czech Republic.
Vasku
Anna
A
Department of Pathophysiology, Faculty of Medicine, Masaryk University, Komenského nám. 2, 662 43, Brno, Czech Republic.
D000328:Adult; D016022:Case-Control Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D020382:Interleukin-18 / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012221:Rhinitis, Allergic, Perennial / Q000235:genetics*; D006255:Rhinitis, Allergic, Seasonal / Q000235:genetics*
D020382:Interleukin-18; D007073:Immunoglobulin E