Predicted epitopes of Lig A of Leptospira interrogans by bioinformatics method: a clue for further vaccine development.
Leptospirosis is an important tropical infection. Providing sufficient antibiotic drugs and development and approval of new vaccines are the control of the possible emerging pandemic of this pathogen. For Leptospira interrogans, leptospiral Ig-like A (LigA) LigA may have value in serodiagnosis or as a protective immunogen in novel vaccines. Here, the author reports the preliminary data from the computation analysis of Lig A to find potential T-cell epitopes using a new bioinformatics tool. Among all alleles, the results from A0203, A0301 and DRB0101 show significant lower IC50 than other alleles. The 307FCISIFLSM315 corresponding to DRB0101 allele is the peptide with the best binding affinity. The determined peptides are useful for further vaccine development because it can reduce the time and minimize the total number of required tests to find the possible proper epitopes, the target for vaccine development.
Wiwanitkit
Viroj
V
Department of Laboratory Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand. wviroj@yahoo.com <wviroj@yahoo.com>
D000483:Alleles; D000596:Amino Acids; D000942:Antigens, Bacterial / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology*; D019295:Computational Biology / Q000379:methods*; D018984:Epitopes, T-Lymphocyte / Q000235:genetics / Q000276:immunology*; D007921:Leptospira interrogans / Q000235:genetics / Q000276:immunology*
D000596:Amino Acids; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D018984:Epitopes, T-Lymphocyte; C487005:LigA-m protein, Leptospira interrogans