Effects of dietary broccoli on human in vivo caffeine metabolism: a pilot study on a group of Jordanian volunteers.
OBJECTIVE
Induction or inhibition of cytochrome P450 (CYP) enzyme activities, enzymes that activate or detoxify xenobiotics, is one mechanism by which vegetables may alter cancer risk. As the effect of food on CYP enzyme activities have not been studied in the Jordanian population, we examined the effect of supplementing the diet with broccoli on CYP1A2 and CYP2A6 activities.


METHODS
Five men and five women, non-smokers, consumed a standard diet of broccoli (500 g) for 6 days. Enzyme activities were determined by measuring urinary metabolite ratios after a 100 mg caffeine tablet on the seventh day.


RESULTS
The mean CYP1A2 activity for men (21.1+/-3.2) was significantly lower than that for women (27.6+/-1.6) before the consumption of broccoli (P<0.05). These activities were significantly induced in both men (52.5+/-6.6) and women (36.6+/-8.4) after a standard diet of broccoli (P<0.005). Similarly, the mean value of CYP2A6 activity for men was 0.061+/-0.040 and for women, 0.144+/-0.039 before consumption of broccoli, which were significantly different (P<0.05). The activity of CYP2A6 was induced in both groups significantly after broccoli consumption (P<0.05). The mean value for men was 0.193+/-0.02 and for women, 0.214+/-0.064.


CONCLUSIONS
Our study on a group of Jordanians confirmed the well-established observation that broccoli induces CYP1A2 activity. This study also demonstrates the effect of gender and broccoli consumption on CYP2A6 activity in Jordanians.
Hakooz
Nancy
N
Faculty of Pharmacy, University of Jordan, Amman 11942 Jordan. nhakooz@ju.edu.jo
Hamdan
Imad
I
D000328:Adult; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism; D001937:Brassica; D002110:Caffeine / Q000493:pharmacokinetics* / Q000652:urine; D019388:Cytochrome P-450 CYP1A2 / Q000378:metabolism; D065637:Cytochrome P-450 CYP2A6; D004032:Diet; D005260:Female; D006801:Humans; D007597:Jordan; D008297:Male; D008875:Middle Aged; D006899:Mixed Function Oxygenases / Q000378:metabolism; D012737:Sex Factors
D002110:Caffeine; D006899:Mixed Function Oxygenases; D001189:Aryl Hydrocarbon Hydroxylases; C504573:CYP1A2 protein, human; C585710:CYP2A6 protein, human; D019388:Cytochrome P-450 CYP1A2; D065637:Cytochrome P-450 CYP2A6