Antibody catalyzed hydrolysis of a quorum sensing signal found in Gram-negative bacteria.
N-(3-Oxo-acyl) homoserine lactones are used by Gram-negative bacteria to signal the establishment of specific population densities and coordinate population-wide gene expression. Herein we report the antibody-catalyzed hydrolysis of N-(3-oxo-acyl) homoserine lactone (AHL) using a reactive immunization strategy with a squaric monoester monoamide hapten. Kinetic analysis of the most efficient antibody revealed a modest k(cat), with AHL hydrolysis competitively inhibited by original squaric monoester monoamide hapten. These studies suggest that antibody catalysis could provide a new avenue for blocking quorum sensing in bacteria.
De Lamo Marin
Sandra
S
Department of Chemistry, The Skaggs Institute for Chemical Biology, and Worm Institute for Research and Medicine (WIRM), The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Xu
Yang
Y
Meijler
Michael M
MM
Janda
Kim D
KD
D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000502:physiology; D000907:Antibodies, Bacterial / Q000494:pharmacology*; D002384:Catalysis; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006868:Hydrolysis; D007700:Kinetics; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D011710:Pyocyanine / Q000096:biosynthesis; D053038:Quorum Sensing / Q000187:drug effects*; D015398:Signal Transduction / Q000187:drug effects*
D000907:Antibodies, Bacterial; C088386:homoserine lactone; D011710:Pyocyanine; D015107:4-Butyrolactone