A method for the quantitative determination of anthraquinones and alkaloids in cell and tissue cultures of cinchona sp.
A procedure is described for the extraction of both anthraquinones and alkaloids from tissue culture material of CINCHONA species. Using this extraction method, it is possible to quantitatively determine the two groups of secondary metabolites produced in CINCHONA tissue cultures.
Wijnsma
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Biotechnology Delft Leiden (BDL), Section of Pharmacognosy, Center for Bio-Pharmaceutical Sciences, Leiden University, Gorlaeus Laboratories, P. O. Box 9502, 2300 RA Leiden, The Netherlands.
van Vliet
T B
TB
Harkes
P A
PA
van Groningen
H J
HJ
van der Heijden
R
R
Verpoorte
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R
Svendsen
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AB