title: Centromere protein H is a novel prognostic marker for nasopharyngeal carcinoma progression and overall patient survival.
abstract: OBJECTIVE
  The aim of the present study was to analyze the expression of Centromere protein H (CENP-H), one of the fundamental components of the human active kinetochore, in nasopharyngeal carcinoma (NPC) and to correlate it with clinicopathologic data, including patient survival.
  
  
  METHODS
  Using reverse transcription-PCR and Western blot, we detected the expression of CENP-H in normal nasopharyngeal epithelial cells, immortalized nasopharyngeal epithelial cell lines, and NPC cell lines. Using immunohistochemistry, we analyzed CENP-H protein expression in 160 clinicopathologically characterized NPC cases. Statistical analyses were applied to test for prognostic and diagnostic associations.
  
  
  RESULTS
  Reverse transcription-PCR and Western blot showed that the expression level of CENP-H was higher in NPC cell lines and in immortalized nasopharyngeal epithelial cells than in the normal nasopharyngeal epithelial cell line at both transcriptional and translational levels. By immunohistochemical analysis, we found that 76 of 160 (47.5%) paraffin-embedded archival NPC biopsies showed high expression of CENP-H. Statistical analysis showed that there was a significant difference of CENP-H expression in patients categorized according to clinical stage (P = 0.024) and T classification (P = 0.027). Patients with higher CENP-H expression had shorter overall survival time, whereas patients with lower CENP-H expression had better survival. A prognostic value of CENP-H was also found of the subgroup of N(0)-N(1) tumor classification. Multivariate analysis showed that CENP-H expression was an independent prognostic indicator for patient's survival.
  
  
  CONCLUSIONS
  Our results suggest that CENP-H protein is a valuable marker of NPC progression. High CENP-H expression is associated with poor overall survival in NPC patients.
authors.affiliation: State Key Laboratory of Oncology in Southern China and Department of Experimental Research, Sun Yat-sen University Cancer Center, 651 Dongfeng Road East, Guangzhou 510060, China.
authors.forename: Wen-Ting
authors.initials: WT
authors.lastname: Liao
authors.forename: Li-Bing
authors.initials: LB
authors.lastname: Song
authors.forename: Hui-Zhong
authors.initials: HZ
authors.lastname: Zhang
authors.forename: Xing
authors.initials: X
authors.lastname: Zhang
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.forename: Wan-Li
authors.initials: WL
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Feng
authors.forename: Bao-Hong
authors.initials: BH
authors.lastname: Guo
authors.forename: Hai-Qiang
authors.initials: HQ
authors.lastname: Mai
authors.forename: Su-Mei
authors.initials: SM
authors.lastname: Cao
authors.forename: Man-Zhi
authors.initials: MZ
authors.lastname: Li
authors.forename: Hai-De
authors.initials: HD
authors.lastname: Qin
authors.forename: Yi-Xin
authors.initials: YX
authors.lastname: Zeng
authors.forename: Mu-Sheng
authors.initials: MS
authors.lastname: Zeng
mesh_terms: D000328:Adult; D002277:Carcinoma / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology; D002681:China; D002868:Chromosomal Proteins, Non-Histone / Q000096:biosynthesis*; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology; D011379:Prognosis; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: C121933:CENPH protein, human; D002868:Chromosomal Proteins, Non-Histone