Direct optical visualization of DNA-DNA interaction by nanoparticle-capture on resonant PET-films.
Based on the understanding of the absorption behavior of metal nanoparticles we aimed at the direct detection of sub-monomolecular layers of DNA with the naked eye. This extremely sensitive detection needs optical amplification techniques to be used in replacement of nanoparticle-aggregates applied e.g., in agglutination assays. We focus on the nanolayer-coated metallized-PET-chip setup and on the synthesis of DNA-nanoparticle conjugates suitable for 'resonance enhanced absorption'-point of care-tests and the application of those particles in the direct visualization of DNA-DNA binding events. Stabilization of nanoparticles and their sequence specific binding was proven with direct optical visibility of sub-monolayers of colored nanoclusters. Synthetic routes leading to suitable conjugates as well as stability tests and a biorecognition test are described in detail adding to the repertoire of tools that contribute to the application of nanoparticles in novel nano-enhanced devices.
Bauer
Maria
M
Attophotonics Biosciences GmbH, Viktor Kaplan Strasse 2, 2700 Wiener Neustadt, Austria.
Haglmüller
Jakob
J
Pittner
Fritz
F
Schalkhammer
Thomas
T
D001672:Biocompatible Materials / Q000737:chemistry; D003460:Crystallization / Q000379:methods; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008567:Membranes, Artificial; D008856:Microscopy, Fluorescence / Q000379:methods*; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D011093:Polyethylene Terephthalates / Q000737:chemistry*; D013499:Surface Properties
D001672:Biocompatible Materials; D046911:Macromolecular Substances; D008567:Membranes, Artificial; D011093:Polyethylene Terephthalates; D004247:DNA