Comparison of in vitro toxicity of single vs double exposures of indocyanine green in human retinal pigment epithelial cell cultures.
OBJECTIVE
To compare the in vitro effect of a single brief indocyanine green (ICG) exposure with a double exposure on retinal pigment epithelial (RPE) cells.


METHODS
In vitro laboratory experimental study.


METHODS
Human ARPE-19 cells were exposed to a single dilute ICG exposure (0.5 mg/ml) or to two sequential exposures of identical volume and concentration. Viability was measured with a mitochondrial dehydrogenase assay and compared with nonexposed control cells.


RESULTS
Cell viability was not statistically different between the single-exposed, double-exposed, or control cell populations.


CONCLUSIONS
When used intraoperatively to stain the internal limiting membrane, dilute ICG dye sometimes does not adequately enhance visualization of the internal limiting membrane. Occasionally, it is necessary to repeat the dye exposure to achieve the desired visibility. In this study, RPE cells subjected to two consecutive short exposures of ICG showed no marked difference in cell survival when compared with cells exposed to a single application of ICG and to control cells.
Proulx
Alain A
AA
Ivey Eye Institute, London, Ontario, Canada.
Gonder
John R
JR
D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents / Q000633:toxicity*; D005562:Formazans; D006801:Humans; D007208:Indocyanine Green / Q000633:toxicity*; D008928:Mitochondria / Q000201:enzymology; D010088:Oxidoreductases / Q000378:metabolism; D010857:Pigment Epithelium of Eye / Q000187:drug effects*; D013778:Tetrazolium Salts
D004396:Coloring Agents; D005562:Formazans; D013778:Tetrazolium Salts; C079782:MTT formazan; D010088:Oxidoreductases; D007208:Indocyanine Green