title: Effects of alpha-phosphoglucomutase deficiency on cell wall properties and fitness in Streptococcus gordonii.
abstract: Streptococcus gordonii alpha-phosphoglucomutase, which converts glucose 6-phosphate to glucose 1-phosphate, is encoded by pgm. The pgm transcript is monocistronic and is initiated from a sigma(A)-like promoter. Mutants with a gene disruption in pgm exhibited an altered cell wall muropeptide pattern and a lower teichoic acid content, and had reduced fitness both in vitro and in vivo. In vitro, the reduced fitness included reduced growth, reduced viability in the stationary phase and increased autolytic activity. In vivo, the pgm-deficient strain had a lower virulence in a rat model of experimental endocarditis.
authors.affiliation: Département de Microbiologie Fondamentale, Bâtiment Biophore, Université de Lausanne, Quartier UNIL-Sorge, CH-1015 Lausanne, Switzerland.
authors.forename: Alain
authors.initials: A
authors.lastname: Bizzini
authors.affiliation: Département de Microbiologie Fondamentale, Bâtiment Biophore, Université de Lausanne, Quartier UNIL-Sorge, CH-1015 Lausanne, Switzerland.
authors.forename: Paul
authors.initials: P
authors.lastname: Majcherczyk
authors.affiliation: Département de Microbiologie Fondamentale, Bâtiment Biophore, Université de Lausanne, Quartier UNIL-Sorge, CH-1015 Lausanne, Switzerland.
authors.forename: Siham
authors.initials: S
authors.lastname: Beggah-Möller
authors.affiliation: Département de Microbiologie Fondamentale, Bâtiment Biophore, Université de Lausanne, Quartier UNIL-Sorge, CH-1015 Lausanne, Switzerland.
authors.forename: Blazenka
authors.initials: B
authors.lastname: Soldo
authors.affiliation: Département de Microbiologie Fondamentale, Bâtiment Biophore, Université de Lausanne, Quartier UNIL-Sorge, CH-1015 Lausanne, Switzerland.
authors.forename: José M
authors.initials: JM
authors.lastname: Entenza
authors.affiliation: Département de Microbiologie Fondamentale, Bâtiment Biophore, Université de Lausanne, Quartier UNIL-Sorge, CH-1015 Lausanne, Switzerland.
authors.forename: Muriel
authors.initials: M
authors.lastname: Gaillard
authors.affiliation: Département de Microbiologie Fondamentale, Bâtiment Biophore, Université de Lausanne, Quartier UNIL-Sorge, CH-1015 Lausanne, Switzerland.
authors.forename: Philippe
authors.initials: P
authors.lastname: Moreillon
authors.affiliation: Département de Microbiologie Fondamentale, Bâtiment Biophore, Université de Lausanne, Quartier UNIL-Sorge, CH-1015 Lausanne, Switzerland.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Lazarevic
mesh_terms: D001483:Base Sequence; D002473:Cell Wall / Q000737:chemistry* / Q000378:metabolism; D004697:Endocarditis, Bacterial / Q000382:microbiology / Q000503:physiopathology; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D010733:Phosphoglucomutase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013290:Streptococcal Infections / Q000382:microbiology / Q000503:physiopathology; D013291:Streptococcus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity; D014774:Virulence
chemical_list: D010733:Phosphoglucomutase