Simple method for the extraction and reversed-phase high-performance liquid chromatographic analysis of carotenoid pigments from red yeasts (Basidiomycota, Fungi).
A simple method for the extraction of carotenoid pigments from frozen wet cells of red yeasts (Basidiomycota) and their analysis by reversed-phase HPLC using a C(18) column and a water/acetone solvent system is described. Typical red yeast carotenoids belonging to an oxidative series from the monocyclic gamma-carotene to 2-hydroxytorularhodin and from the bicyclic beta-carotene to astaxanthin were separated. Pigment identity was confirmed by LC-atmospheric pressure chemical ionisation (APCI) mass spectrometry using similar chromatographic conditions.
Weber
Roland W S
RW
Lehrbereich Biotechnologie, Technische Universität Kaiserslautern, Erwin-Schroedinger-Str. 56, D-67663 Kaiserslautern, Germany.
Anke
Heidrun
H
Davoli
Paolo
P
D001487:Basidiomycota / Q000737:chemistry*; D002338:Carotenoids / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015394:Molecular Structure; D015203:Reproducibility of Results; D024341:Xanthophylls / Q000032:analysis / Q000737:chemistry; D019207:beta Carotene / Q000032:analysis / Q000737:chemistry
D024341:Xanthophylls; D019207:beta Carotene; D002338:Carotenoids; C078922:torulene; C005948:astaxanthine; C078923:torularhodin