Expression of ABCA2 protein in both non-myelin-forming and myelin-forming Schwann cells in the rodent peripheral nerve.
We previously reported that ABCA2, of the A subclass of the ATP-binding cassette (ABC) transporter superfamily, is expressed in mature oligodendrocytes and Schwann cells, the cells responsible for myelination in the brain and the peripheral nerve, respectively. However, unidentified cells expressing ABCA2 also were found. Here, we provide evidence for the expression of ABCA2 in the rodent sciatic nerve not only in Schwann cells, which express the Schwann cell marker S100beta and a zinc finger transcription factor Krox20 (a marker for myelin-forming Schwann cells), but also in Krox20-negative cells, which express glial fibrillary acidic protein (GFAP), a cell adhesion molecule L1, and S100beta weekly. We also analyzed developmental changes in ABCA2 expression in Schwann cells. The expression of ABCA2 in Krox20+/S100beta+ Schwann cells was found initially in rat facial nerve at postnatal day (PD) 8, in half (52.4%) of the cells showing myelinization at PD 14, and in all of the cells in the adult stage. These results demonstrate that ABCA2 is expressed in non-myelin-forming as well as in myelin-forming Schwann cells, and that ABCA2 may be involved in transport of a substance associated with cellular maturation rather than initial myelin formation in both types of Schwann cells.
Saito
Takashi
T
Department of Physiology, Akita University School of Medicine, Akita 010-8543, Japan.
Yamada
Katsuya
K
Wang
Yan
Y
Tanaka
Yukiko
Y
Ohtomo
Kazuo
K
Ishikawa
Kazuo
K
Inagaki
Nobuya
N
D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D000375:Aging / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D051766:Early Growth Response Protein 1 / Q000378:metabolism; D005154:Facial Nerve / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009186:Myelin Sheath / Q000378:metabolism*; D009413:Nerve Fibers, Myelinated / Q000378:metabolism / Q000648:ultrastructure; D009414:Nerve Growth Factors / Q000378:metabolism; D039842:Neural Cell Adhesion Molecule L1 / Q000378:metabolism; D010525:Peripheral Nerves / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000378:metabolism; D012583:Schwann Cells / Q000166:cytology / Q000378:metabolism*; D012584:Sciatic Nerve / Q000166:cytology / Q000254:growth & development / Q000378:metabolism
D018528:ATP-Binding Cassette Transporters; C498824:Abca2 protein, mouse; D015415:Biomarkers; D051766:Early Growth Response Protein 1; C486109:Egr1 protein, mouse; D005904:Glial Fibrillary Acidic Protein; D009414:Nerve Growth Factors; D039842:Neural Cell Adhesion Molecule L1; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins