title: [Apoptosis and gene FasL expression induced by carbon disulfide in rat sertoli cells].
abstract: OBJECTIVE
  To study apoptosis and gene FasL expression induced by carbon disulfide in sertoli cells of male rats.
  
  
  METHODS
  Sertoli cells were exposed to different concentrations of CS(2) (0, 0.36, 0.72, 1.44 micromol/ml) for 24 hours. Survival rate, apoptosis rate, expression level of gene FasL were measured using MTT, FCM, and RT-PCR methods respectively.
  
  
  RESULTS
  Sertoli cell survival rate decreased as the concentration of CS(2) increased. The survival rate (73.34% +/- 1.39%) was significantly lower than the control group (99.98% +/- 5.48%) when the concentration of CS(2) > or = 1.44 micromol/ml (P < 0.05). Apoptosis rate increased as the CS(2) concentration increased. Apoptosis rate (7.93% +/- 0.43%) was significantly higher when the concentration of CS(2) > or = 1.44 micromol/ml (P < 0.05). Expression level of the FasL significantly increased as the concentrations of CS(2) (P < 0.05).
  
  
  CONCLUSIONS
  CS(2) is cytotoxic to sertoli cells. It could cause apoptosis of sertoli cells.
authors.affiliation: Department of Labor Health & Environment Health, Tongji Medical College of Huazhong University of Science & Technology, Wuhan 430030, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Deng
authors.forename: Guo-Yuan
authors.initials: GY
authors.lastname: Chen
authors.forename: Jia-Jia
authors.initials: JJ
authors.lastname: Ji
authors.forename: Hao
authors.initials: H
authors.lastname: Tan
authors.forename: Song
authors.initials: S
authors.lastname: Wang
authors.forename: Feng-Xian
authors.initials: FX
authors.lastname: Hu
authors.forename: Lian-Hua
authors.initials: LH
authors.lastname: Chai
authors.forename: Si-Hai
authors.initials: SH
authors.lastname: Liu
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002246:Carbon Disulfide / Q000633:toxicity*; D002460:Cell Line; D002470:Cell Survival; D053222:Fas Ligand Protein / Q000378:metabolism*; D008297:Male; D051381:Rats; D012708:Sertoli Cells / Q000187:drug effects* / Q000378:metabolism*; D013737:Testis / Q000166:cytology
chemical_list: D053222:Fas Ligand Protein; C505662:Faslg protein, rat; D002246:Carbon Disulfide