title: Bacillus lehensis sp. nov., an alkalitolerant bacterium isolated from soil.
abstract: A Gram-positive, endospore-forming, alkalitolerant bacterial strain, designated MLB2T, was isolated from soil from Leh, India, and was subjected to a polyphasic taxonomic study. The strain exhibited phenotypic properties that included chemotaxonomic characteristics consistent with its classification in the genus Bacillus. Growth was observed at pH 7.0-11.0, but not at pH 6.0. The DNA G+C content was 41.4 mol%. The highest level of 16S rRNA gene sequence similarity was with Bacillus oshimensis JCM 12663T (98.8 %). However, DNA-DNA hybridization experiments indicated low levels of genomic relatedness with the type strains of B. oshimensis (62 %), Bacillus patagoniensis (55 %), Bacillus clausii (51 %) and Bacillus gibsonii (34 %), the species with which strain MLB2T formed a coherent cluster (based on the results of the phylogenetic analysis). On the basis of the phenotypic characteristics and genotypic distinctiveness of strain MLB2T, it should be classified within a novel species of Bacillus, for which the name Bacillus lehensis sp. nov. is proposed. The type strain is MLB2T (=MTCC 7633T=JCM 13820T).
authors.affiliation: Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology, Sector-39A, Chandigarh 160036, India.
authors.forename: A
authors.initials: A
authors.lastname: Ghosh
authors.affiliation: Microbiology, University of Delhi, South Campus, Benito Juarez Road, New Delhi 110021, India.
authors.forename: M
authors.initials: M
authors.lastname: Bhardwaj
authors.affiliation: Microbiology, University of Delhi, South Campus, Benito Juarez Road, New Delhi 110021, India.
authors.forename: T
authors.initials: T
authors.lastname: Satyanarayana
authors.affiliation: Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology, Sector-39A, Chandigarh 160036, India.
authors.forename: M
authors.initials: M
authors.lastname: Khurana
authors.affiliation: Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology, Sector-39A, Chandigarh 160036, India.
authors.forename: S
authors.initials: S
authors.lastname: Mayilraj
authors.affiliation: Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology, Sector-39A, Chandigarh 160036, India.
authors.forename: R K
authors.initials: RK
authors.lastname: Jain
mesh_terms: D000468:Alkalies / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001407:Bacillus / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial; D020459:Genes, rRNA / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D007194:India; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012988:Soil Microbiology
chemical_list: D000468:Alkalies; D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S