title: Investigation of in vivo measurement of cerebral cytochrome-c-oxidase redox changes using near-infrared spectroscopy in patients with orthostatic hypotension.
abstract: We have previously used a continuous four-wavelength near-infrared spectrometer to measure changes in the cerebral concentrations of oxy-haemoglobin (Delta[HbO(2)] and deoxy-haemoglobin (Delta[HHb]) during head-up tilt in patients with primary autonomic failure. The measured changes in light attenuation also allow calculation of changes in the concentration of oxidized cytochrome-c-oxidase (Delta[(ox)CCO]), and this paper analyses the Delta[(ox)CCO] during the severe episodes of orthostatic hypotension produced by this experimental protocol. We studied 12 patients during a passive change in position from supine to a 60 degrees head-up tilt. The challenge caused a reduction in mean blood pressure of 59.93 (+/-26.12) mmHg (Mean (+/-SD), p < 0.0001), which was associated with a reduction in the total concentration of haemoglobin (Delta[HbT] = Delta[HbO(2)] + Delta[HHb]) of 5.02 (+/-3.81) microM (p < 0.0001) and a reduction in the haemoglobin difference concentration (Delta[Hb(diff)] = Delta[HbO(2)] - Delta[HHb]) of 14.4 (+/-6.73) microM (p < 0.0001). We observed a wide range of responses in Delta[(ox)CCO]. Six patients demonstrated a drop in Delta[(ox)CCO] (0.17 +/- 0.15 microM); four patients demonstrated no change (0.01 +/- 0.12 microM) and two patients showed an increase in Delta[(ox)CCO] (0.21 +/- 0.01 microM). Investigation of the association between the changes in concentrations of haemoglobin species and the Delta[(ox)CCO] for each patient show a range of relationships. This suggests that a simple mechanism for crosstalk, which might produce artefactual changes in [(ox)CCO], is not present between the haemoglobin and the (ox)CCO NIRS signals. Further investigation is required to determine the clinical significance of the changes in [(ox)CCO].
authors.affiliation: Department of Medical Physics and Bioengineering, Malet Place Engineering Building, University College London, Gower Street, London, UK. iliastac@medphys.ucl.ac.uk
authors.forename: I
authors.initials: I
authors.lastname: Tachtsidis
authors.forename: M
authors.initials: M
authors.lastname: Tisdall
authors.forename: T S
authors.initials: TS
authors.lastname: Leung
authors.forename: C E
authors.initials: CE
authors.lastname: Cooper
authors.forename: D T
authors.initials: DT
authors.lastname: Delpy
authors.forename: M
authors.initials: M
authors.lastname: Smith
authors.forename: C E
authors.initials: CE
authors.lastname: Elwell
mesh_terms: D000328:Adult; D000368:Aged; D000465:Algorithms; D001794:Blood Pressure / Q000502:physiology; D001921:Brain / Q000201:enzymology*; D003627:Data Interpretation, Statistical; D003576:Electron Transport Complex IV / Q000737:chemistry*; D005260:Female; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D007024:Hypotension, Orthostatic / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D010108:Oxyhemoglobins / Q000378:metabolism; D019265:Spectroscopy, Near-Infrared; D016683:Supine Position / Q000502:physiology
chemical_list: D006454:Hemoglobins; D010108:Oxyhemoglobins; C100003:deoxyhemoglobin; D003576:Electron Transport Complex IV