title: Short-term in vitro culture of field isolates of Plasmodium vivax using umbilical cord blood.
abstract: Plasmodium vivax research has been hampered by the lack of technology for culturing this parasite. Culturing P. vivax is difficult because the parasite selectively invades reticulocytes. Here we describe a modified procedure to establish and maintain short-term cultures of freshly collected P. vivax parasites using reticulocyte-enriched cord blood. Using this method, parasites could be cultured for a month. Manipulation of the culture allowed procurement of synchronized stages of the parasite. This short-term culture method can be easily adapted to study various aspects of the parasite biology.
authors.affiliation: Department of Pathobiology, Mahidol University, Bangkok, Thailand. scrud@mahidol.ac.th
authors.forename: Rachanee
authors.initials: R
authors.lastname: Udomsangpetch
authors.forename: Sangdao
authors.initials: S
authors.lastname: Somsri
authors.forename: Tasanee
authors.initials: T
authors.lastname: Panichakul
authors.forename: Kesinee
authors.initials: K
authors.lastname: Chotivanich
authors.forename: Jeeraphat
authors.initials: J
authors.lastname: Sirichaisinthop
authors.forename: Zhaoqing
authors.initials: Z
authors.lastname: Yang
authors.forename: Liwang
authors.initials: L
authors.lastname: Cui
authors.forename: Jetsumon
authors.initials: J
authors.lastname: Sattabongkot
mesh_terms: D000818:Animals; D005260:Female; D005312:Fetal Blood; D006432:Hemochromatosis / Q000097:blood; D006801:Humans; D008018:Life Cycle Stages; D016780:Malaria, Vivax / Q000469:parasitology*; D010966:Plasmodium vivax / Q000254:growth & development* / Q000302:isolation & purification; D012156:Reticulocytes / Q000469:parasitology*