Profiling of glycosidase activities using coumarin-conjugated glycoside cocktails.
Glycosidases are a large subgroup of carbohydrate-processing enzymes that hydrolytically cleave the glycosidic bond. Glycans formed by the action of glycosidases are involved in various biological processes. Genetic abnormalities in glycosidases are associated with inherited diseases. Thus, characterization of the catalytic activities of glycosidases is of great importance. Herein, we describe a simple and rapid approach for determining glycosidase activity profiles using coumarin-conjugated glycoside cocktails. [reaction: see text].
Park
Sungjin
S
Department of Chemistry, Yonsei University, Seoul 120-749, Korea.
Shin
Injae
I
D001236:Aspergillus oryzae / Q000201:enzymology; D001407:Bacillus / Q000201:enzymology; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000737:chemistry*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism*; D006027:Glycosides / Q000737:chemistry*; D007716:Kluyveromyces / Q000201:enzymology; D011134:Polysaccharides / Q000737:chemistry
D003374:Coumarins; D006027:Glycosides; D011134:Polysaccharides; D006026:Glycoside Hydrolases