title: Solution structure of an atypical WW domain in a novel beta-clam-like dimeric form.
abstract: The WW domain is known as one of the smallest protein modules with a triple-stranded beta-sheet fold. Here, we present the solution structure of the second WW domain from the mouse salvador homolog 1 protein. This WW domain forms a homodimer with a beta-clam-like motif, as evidenced by size exclusion chromatography, analytical ultracentrifugation and NMR spectroscopy. While typical WW domains are believed to function as monomeric modules that recognize proline-rich sequences, by using conserved aromatic and hydrophobic residues that are solvent-exposed on the surface of the beta-sheet, this WW domain buries these residues in the dimer interface.
authors.affiliation: Genomic Sciences Center, RIKEN, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ohnishi
authors.forename: Peter
authors.initials: P
authors.lastname: Güntert
authors.forename: Seizo
authors.initials: S
authors.lastname: Koshiba
authors.forename: Tadashi
authors.initials: T
authors.lastname: Tomizawa
authors.forename: Ryogo
authors.initials: R
authors.lastname: Akasaka
authors.forename: Naoya
authors.initials: N
authors.lastname: Tochio
authors.forename: Manami
authors.initials: M
authors.lastname: Sato
authors.forename: Makoto
authors.initials: M
authors.lastname: Inoue
authors.forename: Takushi
authors.initials: T
authors.lastname: Harada
authors.forename: Satoru
authors.initials: S
authors.lastname: Watanabe
authors.forename: Akiko
authors.initials: A
authors.lastname: Tanaka
authors.forename: Mikako
authors.initials: M
authors.lastname: Shirouzu
authors.forename: Takanori
authors.initials: T
authors.lastname: Kigawa
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yokoyama
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D019281:Dimerization; D066298:In Vitro Techniques; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012996:Solutions; D013816:Thermodynamics
chemical_list: D018797:Cell Cycle Proteins; D011994:Recombinant Proteins; C485935:Sav1 protein, mouse; D012996:Solutions