Endothelial cell-mediated regulation of aldosterone release from human adrenocortical cells.
Endothelial cells play an important role in the development and functioning of endocrine tissue and endothelial cell-derived factors have been shown to regulate mineralocorticoid release in bovine adrenal cells. In the present study, we analysed the role of human endothelial cells in the synthesis and release of aldosterone from adrenocortical cells (NCI-H295R). Endothelial cell-induced aldosterone release was rapid and lasted as a long-term effect over a period of 48 h. This stimulant effect was influenced by the duration of endothelial cell conditioning and decreased linearly with increasing dilutions of the conditioned medium. At the molecular level, an increase in the mRNA transcripts of aldosterone synthase and StAR could be observed. Cellular interaction with endothelial cell-factors enhanced the activation of CRE, and the promoter activity of both StAR and SF-1 reporter genes. In conclusion, human endothelial cells are important intra-adrenal regulators of human aldosterone synthesis and release.
Ansurudeen
Ishrath
I
Department of Medicine III, Carl Gustav Carus Medical School, University of Technology Dresden, 01307 Dresden, Germany. Ishrath.Ansurudeen@uniklinikum-dresden.de
Kopprasch
Steffi
S
Ehrhart-Bornstein
Monika
M
Bornstein
Stefan R
SR
Willenberg
Holger S
HS
D000302:Adrenal Cortex / Q000166:cytology / Q000378:metabolism*; D000450:Aldosterone / Q000378:metabolism*; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D000242:Cyclic AMP / Q000378:metabolism; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D019405:Cytochrome P-450 CYP11B2 / Q000235:genetics; D042783:Endothelial Cells / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D010750:Phosphoproteins / Q000235:genetics; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics; D020218:Response Elements / Q000235:genetics; D054339:Steroidogenic Factor 1; D014157:Transcription Factors / Q000235:genetics; D014470:Umbilical Cord / Q000166:cytology; D000098682:Steroidogenic Acute Regulatory Protein
D017077:Culture Media, Conditioned; D017362:Cyclic AMP Response Element-Binding Protein; D018398:Homeodomain Proteins; D010750:Phosphoproteins; D018160:Receptors, Cytoplasmic and Nuclear; D054339:Steroidogenic Factor 1; D014157:Transcription Factors; D000098682:Steroidogenic Acute Regulatory Protein; D000450:Aldosterone; D000242:Cyclic AMP; D019405:Cytochrome P-450 CYP11B2