Synthesis of Clostridium cellulovorans minicellulosomes by intercellular complementation.
The ability of two strains of bacteria to cooperate in the synthesis of an enzyme complex (a minicellulosome) was examined. Three strains of Bacillus subtilis were constructed to express Clostridium cellulovorans genes engB, xynB, and minicbpA. MiniCbpA, EngB, and XynB were synthesized and secreted into the medium by B. subtilis. When the strains with the minicbpA and engB genes or with xynB were cocultured, minicellulosomes were synthesized, consisting in one case of miniCbpA and EngB and in the second case of miniCbpA and XynB. Both minicellulosomes showed their respective enzymatic activities. We call this phenomenon "intercellular complementation." Interesting implications concerning bacterial cooperation are suggested from these results.
Arai
Takamitsu
T
Section of Molecular and Cellular Biology, University of California, Davis, CA 95616-8535, USA.
Matsuoka
Satoshi
S
Cho
Hee-Yeon
HY
Yukawa
Hideaki
H
Inui
Masayuki
M
Wong
Sui-Lam
SL
Doi
Roy H
RH
D001412:Bacillus subtilis / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D001671:Biochemistry / Q000379:methods; D002352:Carrier Proteins / Q000235:genetics; D020134:Catalytic Domain; D002480:Cellulase / Q000235:genetics; D044603:Cellulosomes / Q000737:chemistry* / Q000378:metabolism*; D003001:Cloning, Molecular; D046974:Clostridium cellulovorans / Q000378:metabolism*; D018920:Coculture Techniques; D043364:Endo-1,4-beta Xylanases / Q000235:genetics; D009097:Multienzyme Complexes / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D013045:Species Specificity; D001617:beta-Glucosidase / Q000235:genetics
D001426:Bacterial Proteins; D002352:Carrier Proteins; D009097:Multienzyme Complexes; C074074:cellulose binding protein A, Clostridium cellulovorans; D001617:beta-Glucosidase; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases; C090001:XynB xylanase