Exoenzymes of Trametes versicolor can metabolize coplanar PCB congeners and hydroxy PCB.
Two fractions containing the oxidase activity toward 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonate) (ABTS) were obtained using ion-exchange DEAE-Sepharose column chromatography of the culture fluid of white-rot fungus, Trametes versicolor. These two fractions can reduce the level of coplanar PCB congeners (Co-PCBs). The ABTS oxidase in the first fraction passed through the DEAE-Sepharose column. The ABTS oxidase in the second fraction was adsorbed to the column at <pH 5.6. Overall, the first fraction has a higher activity to reduce each level of Co-PCBs than the second fraction does. In equally chlorinated congeners, non-ortho congeners were less metabolized than ortho congeners; a 4-hydroxy-pentachlorobiphenyl (hydroxy PeCB) was more susceptible to metabolism than a structurally related non-hydroxy PeCB or HxCB.
Takagi
Shigeyuki
S
Graduate School of Environmental Health, Azabu University, Kanagawa 229-8501, Japan.
Shirota
Chiaki
C
Sakaguchi
Kazuko
K
Suzuki
Jun
J
Sue
Takumi
T
Nagasaka
Hiromitsu
H
Hisamatsu
Shin
S
Sonoki
Shigenori
S
D000818:Animals; D052160:Benzothiazoles / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002852:Chromatography, Ion Exchange; D010088:Oxidoreductases / Q000378:metabolism*; D011078:Polychlorinated Biphenyls / Q000378:metabolism* / Q000633:toxicity; D020072:Polyporales / Q000201:enzymology*; D013451:Sulfonic Acids / Q000302:isolation & purification / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism* / Q000633:toxicity
D052160:Benzothiazoles; D013451:Sulfonic Acids; D014874:Water Pollutants, Chemical; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D011078:Polychlorinated Biphenyls; D010088:Oxidoreductases