Experimental vertical transmission of Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) from brooders to progeny in Macrobrachium rosenbergii and Artemia.
White tail disease (WTD) is a serious problem in hatcheries and nursery ponds of Macrobrachium rosenbergii in India. Experiments were carried out to determine the possibility of vertical transmission of M. rosenbergii nodavirus (MrNV) and extra small virus (XSV) in M. rosenbergii and Artemia. Prawn broodstock inoculated with MrNV and XSV by oral or immersion challenge survived without any clinical signs of WTD. The brooders spawned 5-7 days after inoculation and the eggs hatched. The survival rate of larvae gradually decreased, and 100% mortality was observed at the post-larvae (PL) stage. Whitish muscle, the typical sign of WTD, was seen in advanced larval developmental stages. The ovarian tissue and fertilized eggs were found to be positive for MrNV/XSV by reverse transcriptase-polymerase chain reaction (RT-PCR) whereas the larval stages showed positive by RT nested PCR (nRT-PCR). In Artemia, reproductive cysts and nauplii derived from challenged brooders were normal and survival rates were within the expected range for normal rearing conditions. The reproductive cysts were found to be positive for MrNV/XSV by RT-PCR whereas the nauplii showed MrNV/XSV-positive by nRT-PCR. The PL of M. rosenbergii fed nauplii derived from challenged Artemia brooders died at 9 days post-inoculum with clinical signs of WTD.
Sudhakaran
R
R
Aquaculture Biotechnology Division, Department of Zoology, C. Abdul Hakeem College, Melvisharam, Tamil Nadu, India.
Ishaq Ahmed
V P
VP
Haribabu
P
P
Mukherjee
S C
SC
Sri Widada
J
J
Bonami
J R
JR
Sahul Hameed
A S
AS
D000818:Animals; D001156:Artemia / Q000821:virology*; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D007814:Larva / Q000821:virology; D008018:Life Cycle Stages; D030381:Nodaviridae / Q000302:isolation & purification* / Q000472:pathogenicity*; D010053:Ovary / Q000821:virology; D033541:Palaemonidae / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016019:Survival Analysis; D013997:Time Factors; D014780:Viruses / Q000302:isolation & purification / Q000472:pathogenicity*; D015053:Zygote / Q000821:virology
D017931:DNA Primers