A conditionally immortalized cell line model for the study of human prostatic epithelial cell differentiation.
In the normal human prostate, undifferentiated proliferative cells reside in the basal layer and give rise to luminal secretory cells. There are, however, few epithelial cell lines that have a basal cell phenotype and are able to differentiate. We set out to develop a cell line with these characteristics that would be suitable for the study of the early stages of prostate epithelial cell differentiation. We produced a matched pair of conditionally immortalized prostate epithelial and stromal cell lines derived from the same patient. The growth of these cells is temperature dependent and differentiation can be induced following a rise in culture temperature. Three-dimensional co-cultures of these cell lines elicited gland-like structures reminiscent of prostatic acini. cDNA microarray analysis of the epithelial line demonstrated changes in gene expression consistent with epithelial differentiation. These genes may prove useful as markers for different prostate cell types. The cell lines provide a model system with which to study the process of prostatic epithelial differentiation and stromal-epithelial interactions. This may prove to be useful in the development of differentiation-targeted prostate cancer therapies.
Daly-Burns
Bernadette
B
Prostate Cancer Research Centre, Institute of Urology, University College London, 67 Riding House Street, London W1W 7EJ, UK.
Alam
Tahirah N
TN
Mackay
Alan
A
Clark
Jeremy
J
Shepherd
Christopher J
CJ
Rizzo
Siân
S
Tatoud
Roger
R
O'Hare
Michael J
MJ
Masters
John R
JR
Hudson
David L
DL
D000368:Aged; D002454:Cell Differentiation; D002461:Cell Line, Transformed / Q000166:cytology*; D004847:Epithelial Cells / Q000166:cytology*; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008875:Middle Aged; D008954:Models, Biological; D011467:Prostate / Q000166:cytology / Q000254:growth & development*; D012333:RNA, Messenger / Q000032:analysis; D013696:Temperature
D012333:RNA, Messenger