Detection of rabies virus genes by in-situ polymerase chain reaction.
Rabies is diagnosed by FAT in the impression smears of brain tissues. In this study, an attempt was made to diagnose rabies using in situ polymerase chain reaction (ISPCR). A digoxigenin-labelled double-stranded probe specific for a portion of the 'N' gene of rabies virus was used. Positive signals were identified as blue dots in the intraneuronal and neuropil areas.
Praveena
P Ezhil
PE
Indian Veterinary Research Institute, Izatnagar, Bareilly.
Jayakumar
R
R
Balachandran
C
C
Thirumurugan
G
G
Raj
G Dhinakar
GD
Manohar
B Murali
BM
D000818:Animals; D002493:Central Nervous System Diseases / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D004283:Dog Diseases / Q000175:diagnosis / Q000821:virology*; D004285:Dogs; D005260:Female; D006624:Hippocampus / Q000821:virology; D007150:Immunohistochemistry / Q000662:veterinary; D008297:Male; D019590:Nucleocapsid Proteins / Q000737:chemistry / Q000235:genetics; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D011818:Rabies / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D011820:Rabies virus / Q000235:genetics* / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D013687:Telencephalon / Q000821:virology
D019590:Nucleocapsid Proteins; D012367:RNA, Viral