title: Novel method for preparing spheroplasts from cells with an internal cellulosic cell wall.
abstract: Protoplast and spheroplast preparations allow the transfer of macromolecules into cells and provide the basis for the generation of engineered organisms. Crypthecodinium cohnii cells harvested from polyethylene glycol-containing agar plates possessed significantly lower levels of cellulose in their cortical layers, which facilitated the delivery of fluorescence-labeled oligonucleotides into these cells.
authors.affiliation: Department of Biology, Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong SAR, People's Republic of China.
authors.forename: Alvin C M
authors.initials: AC
authors.lastname: Kwok
authors.forename: Carmen C M
authors.initials: CC
authors.lastname: Mak
authors.forename: Francis T W
authors.initials: FT
authors.lastname: Wong
authors.forename: Joseph T Y
authors.initials: JT
authors.lastname: Wong
mesh_terms: D000818:Animals; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D002482:Cellulose / Q000096:biosynthesis*; D004141:Dinoflagellida / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification / Q000648:ultrastructure*; D016193:G1 Phase / Q000187:drug effects / Q000502:physiology; D016195:G2 Phase; D008560:Membrane Fluidity; D030781:Organisms, Genetically Modified; D010539:Permeability / Q000187:drug effects; D011092:Polyethylene Glycols / Q000494:pharmacology; D011523:Protoplasts / Q000187:drug effects / Q000378:metabolism; D013104:Spheroplasts / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification / Q000648:ultrastructure*; D013194:Staining and Labeling
chemical_list: D011092:Polyethylene Glycols; D002482:Cellulose