Miniaturised isotachophoresis of DNA.
This paper presents the findings of a feasibility study investigating the behaviour of DNA under conditions of miniaturised isotachophoresis. An electrolyte system comprising a leading electrolyte of 5mM perchloric acid at pH 6.0 and a terminating electrolyte of 10mM gallic acid was devised and used to perform isotachophoresis of DNA containing samples on a miniaturised poly(methyl methacrylate) device. Under such conditions it was found that no separation of DNA fragments was observed with the substance migrating instead as a single isotachophoretic zone. Whilst such a result shows the method is unsuitable for analysis DNA it offers significant potential as a means of sample preparation for subsequent analysis using another method. This is because the single zone of DNA formed is preconcentrated to a constant concentration governed by the leading ion and is separated from all species with different effective electrophoretic mobilities.
Prest
Jeff E
JE
School of Chemical Engineering and Analytical Science, The University of Manchester, Manchester, UK. j.prest@manchester.ac.uk
Baldock
Sara J
SJ
Day
Philip J R
PJ
Fielden
Peter R
PR
Goddard
Nicholas J
NJ
Treves Brown
Bernard J
BJ
D000818:Animals; D004247:DNA / Q000302:isolation & purification*; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods*; D015894:Genome, Human; D006801:Humans; D008904:Miniaturization / Q000379:methods*; D019904:Polymethyl Methacrylate; D012474:Salmon / Q000235:genetics
D004247:DNA; D019904:Polymethyl Methacrylate