[Relationship between surface molecules and RelB gene expression in DC2.4 cell line].
OBJECTIVE
To explore the relationship between the expression of the nuclear factor-kappaB transcription factor RelB gene and the surface molecules in DC2.4 cell line.


METHODS
DC2.4 cell line was cultured in complete RPMI 1640 medium, whose morphology was observed with optical microscope and the intracellular structures with transmission electron microscope. Flow cytometry was performed to analyze the surface markers of the cells, including MHC-II, CD86 and CD40, and RelB mRNA expression was detected by RT-PCR.


RESULTS
Under optical microscope, the cells appeared irregular in shape with obvious dendritic cell processes, and electron microscopy revealed homogenous fat drops and phagocytic vesicles in the cytoplasm. Flow cytometry demonstrated low expression levels of MHC-II and CD40, but high level of CD86 molecules. Low-level expression of RelB mRNA was detected by RT-PCR, resembling its expression level in bone marrow-derived DC with immature phenotype.


CONCLUSIONS
DC2.4 is a mouse bone marrow dendritic cell line with strong phagocytic capacity, and the low expression of both RelB gene and surface CD40 molecules suggests an immature dendritic cell line.
Bao
Jie
J
Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. baojiemimi@163.com
Zheng
Lei
L
Zeng
Fang-Yin
FY
Yang
Hong-Ling
HL
Qiu
Yu-Rong
YR
Li
Juan
J
Wang
Qian
Q
D000818:Animals; D019013:CD40 Antigens / Q000096:biosynthesis* / Q000235:genetics; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D005434:Flow Cytometry; D051379:Mice; D008810:Mice, Inbred C57BL; D046529:Microscopy, Electron, Transmission; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051841:Transcription Factor RelB / Q000096:biosynthesis* / Q000235:genetics; D014162:Transfection
D019013:CD40 Antigens; C497594:Relb protein, mouse; D051841:Transcription Factor RelB