title: Huperzine A protects C6 rat glioma cells against oxygen-glucose deprivation-induced injury.
abstract: The protective effects of huperzine A against oxygen-glucose deprivation (OGD)-induced injury in C6 cells were investigated. OGD for 6h and reoxygenation for 6h enhanced phosphorylation and degradation of IkappaBalpha and nuclear translocation of nuclear factor-kappa B (NF-kappaB), triggered overexpression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and nitric oxide (NO) in C6 cells. Along with inhibiting acetylcholinesterase activity, treatment with 1 microM huperzine A inhibited activation of NF-kappaB, attenuated iNOS, COX-2 and NO overexpression, and promoted survival in C6 cells subjected to OGD insult. The protective effects of huperzine A were partly mediated by "cholinergic anti-inflammatory pathway" through alpha7 nicotinic acetylcholine receptor.
authors.affiliation: State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, People's Republic of China.
authors.forename: Zhi Fei
authors.initials: ZF
authors.lastname: Wang
authors.forename: Xi Can
authors.initials: XC
authors.lastname: Tang
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000470:Alkaloids; D000818:Animals; D002038:Bungarotoxins / Q000494:pharmacology; D015687:Cell Hypoxia / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000378:metabolism; D005910:Glioma / Q000201:enzymology / Q000473:pathology*; D005947:Glucose / Q000172:deficiency*; D020672:I-kappa B Proteins / Q000378:metabolism; D008464:Mecamylamine / Q000494:pharmacology; D000072000:NF-KappaB Inhibitor alpha; D009569:Nitric Oxide / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D011392:Proline / Q000031:analogs & derivatives / Q000494:pharmacology; D021381:Protein Transport / Q000187:drug effects; D051381:Rats; D012717:Sesquiterpenes / Q000494:pharmacology*; D013859:Thiocarbamates / Q000494:pharmacology; D051996:Transcription Factor RelA / Q000378:metabolism
chemical_list: D000470:Alkaloids; D002038:Bungarotoxins; D020672:I-kappa B Proteins; C000606206:Nfkbia protein, rat; D012717:Sesquiterpenes; D013859:Thiocarbamates; D051996:Transcription Factor RelA; C050426:huperzine A; C066229:prolinedithiocarbamate; D000072000:NF-KappaB Inhibitor alpha; D009569:Nitric Oxide; D008464:Mecamylamine; D011392:Proline; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; D000110:Acetylcholinesterase; D005947:Glucose