title: Myosin 1E interacts with synaptojanin-1 and dynamin and is involved in endocytosis.
abstract: Myosin 1E is one of two "long-tailed" human Class I myosins that contain an SH3 domain within the tail region. SH3 domains of yeast and amoeboid myosins I interact with activators of the Arp2/3 complex, an important regulator of actin polymerization. No binding partners for the SH3 domains of myosins I have been identified in higher eukaryotes. In the current study, we show that two proteins with prominent functions in endocytosis, synaptojanin-1 and dynamin, bind to the SH3 domain of human Myo1E. Myosin 1E co-localizes with clathrin- and dynamin-containing puncta at the plasma membrane and this co-localization requires an intact SH3 domain. Expression of Myo1E tail, which acts in a dominant-negative manner, inhibits endocytosis of transferrin. Our findings suggest that myosin 1E may contribute to receptor-mediated endocytosis.
authors.affiliation: Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06511, USA. mira.krendel@yale.edu
authors.forename: Mira
authors.initials: M
authors.lastname: Krendel
authors.forename: Emily K
authors.initials: EK
authors.lastname: Osterweil
authors.forename: Mark S
authors.initials: MS
authors.lastname: Mooseker
mesh_terms: D016475:3T3 Cells; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D022163:Clathrin-Coated Vesicles / Q000378:metabolism; D034281:Dynamins / Q000378:metabolism*; D004705:Endocytosis; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation; D051379:Mice; D024461:Myosin Type I / Q000737:chemistry / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D011485:Protein Binding; D021381:Protein Transport; D051381:Rats; D013569:Synapses / Q000378:metabolism; D014020:Tissue Extracts; D014168:Transferrin / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D018909:src Homology Domains
chemical_list: D009419:Nerve Tissue Proteins; D014020:Tissue Extracts; D014168:Transferrin; C097702:synaptojanin; D010744:Phosphoric Monoester Hydrolases; C511011:MYO1E protein, human; D024461:Myosin Type I; D034281:Dynamins