title: The Aedes albopictus inhibitor of apoptosis 1 gene protects vertebrate cells from bluetongue virus-induced apoptosis.
abstract: We sequenced and characterized the inhibitor of apoptosis (iap) 1 gene from Aedes albopictus, designated as Aaiap1. The Aaiap1 gene rescued Spodoptera frugiperda (Sf9) cells from apoptosis when cotransfected with the Drosophila pro-apoptotic hid gene. The antiapoptotic function of the Aaiap1 gene was evaluated in the bluetongue virus (BTV)-induced apoptosis system. BTV infection induced apoptosis in vertebrate cells via the intrinsic apoptotic pathway. This was shown by the translocation of cytochrome C and the second mitochondria-derived activator of caspase (Smac, also known as DIABLO) from the mitochondria and the subsequent activation of caspase-9 and -3. Stable expression of the Aaiap1 gene in derivative baby hamster kidney cells delayed BTV-induced apoptosis by 24 h and reduced the BTV progeny yield by 10-fold. This study provides the first evidence that the mosquito AaIAP1 protein possesses antiapoptotic activity.
authors.affiliation: Division of Geographic Medicine, Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA. liq@uab.edu
authors.forename: Q
authors.initials: Q
authors.lastname: Li
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.forename: B J
authors.initials: BJ
authors.lastname: Blitvich
authors.forename: J
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000330:Aedes / Q000166:cytology / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D001820:Bluetongue virus / Q000502:physiology*; D002460:Cell Line; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism; D013752:Tetracycline; D014779:Virus Replication
chemical_list: D029721:Drosophila Proteins; C108880:HID protein, Drosophila; D051034:Inhibitor of Apoptosis Proteins; D009479:Neuropeptides; D013752:Tetracycline