Effect of testosterone on oxidative stress and cell damage induced by 3-nitropropionic acid in striatum of ovariectomized rats.
This paper evaluates the effects of testosterone (0.5 mg/kg subcutaneously (s.c.) for 8 days) on oxidative stress and cell damage induced by 3-nitropropionic acid (20 mg/kg intraperitoneally (i.p.) for 4 days) in ovariectomized rats. Gonadectomy triggered oxidative damage and cell loss, evaluated by the detection of caspase-3, whereas 3-nitropropionic acid increased the levels of oxidative stress induced by ovariectomy and prompted cell damage characterized by enhanced levels of lactate dehydrogenase. These changes were blocked by testosterone administration. Our results support the following conclusions: i) ovariectomy triggers oxidative and cell damage via caspase-3 in the striatum; ii) 3-nitropropionic acid exacerbates oxidative stress induced by ovariectomy and leads to cell damage characterized by increased levels of lactate dehydrogenase; iii) testosterone administration decreases oxidative stress and cell damage. Additionally, these data support the hypothesis that testosterone might play an important role in the onset and development of neurodegenerative diseases.
Túnez
Isaac
I
Departamento de Bioquimica y Biologia Molecular, Facultad de Medicina, Universidad de Cordoba, Avda. Menendez Pidal s/n, 14004 Cordoba, Spain. fm2tufii@uco.es
Feijóo
Montserrat
M
Collado
Juan A
JA
Medina
Francisco J
FJ
Peña
José
J
Muñoz
María del C
Mdel C
Jimena
Ignacio
I
Franco
Francisco
F
Rueda
Ignacio
I
Muntané
Jordi
J
Montilla
Pedro
P
D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D016923:Cell Death / Q000187:drug effects; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D004336:Drug Antagonism; D005260:Female; D006816:Huntington Disease; D007274:Injections, Intraperitoneal; D007279:Injections, Subcutaneous; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D009498:Neurotoxins / Q000633:toxicity*; D009574:Nitro Compounds / Q000633:toxicity*; D010052:Ovariectomy; D018384:Oxidative Stress / Q000187:drug effects*; D011422:Propionates / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D013739:Testosterone / Q000494:pharmacology*
D000728:Androgens; D009498:Neurotoxins; D009574:Nitro Compounds; D011422:Propionates; D013739:Testosterone; D007770:L-Lactate Dehydrogenase; C015392:3-nitropropionic acid