Teratogenic effects of the interaction acetylsalicylic acid (ASA) and ethanol: morphologic and morphometric evaluation of the lingual epithelium in rat fetuses.
The objective of the present work was to evaluate the teratogenic effects of the interaction between acetylsalicylic acid (ASA) and ethanol on the epithelium of the lingual mucosa in rat fetuses. On the 10th pregnancy day, a single intraperitoneal ethanol dose (2.96 g/kg body weight) (Group I), ASA (200 mg/kg body weight) (Group II) and ASA plus ethanol, in the same doses (Group III), or saline (Group IV - control), were administrated. The epithelial alterations were assessed by means of histological and morphometric methods, on posterior dorsal, anterior dorsal and ventral regions of the tongue. ASA reduced, in rat fetuses, the ethanol deleterious effects on nuclear size in the epithelial prickle cell of the lingual mucosa. On the other hand, ASA did not influence the effects of ethanol in both epithelial layers of the lingual mucosa, when the nuclear shape, cell volume or epithelial layers thickness were evaluated.
Marinho
S A
SA
Department of Oral and Maxillofacial Surgery, School of Dentistry of Ribeirão Preto, University of São Paulo, 14040-904 Ribeirão Preto, SP, Brazil.
Sala
M A
MA
Lopes
R A
RA
de Moraes Grisi
M F
MF
Novaes
A B
AB
de Souza
S L S
SL
Taba
M
M
D000014:Abnormalities, Drug-Induced / Q000662:veterinary*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000633:toxicity; D001241:Aspirin / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004847:Epithelial Cells; D000431:Ethanol / Q000633:toxicity*; D005260:Female; D005333:Fetus / Q000002:abnormalities* / Q000187:drug effects; D018811:Maternal Exposure / Q000009:adverse effects; D009061:Mouth Mucosa / Q000166:cytology / Q000187:drug effects / Q000473:pathology; D011247:Pregnancy; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013723:Teratogens; D013997:Time Factors
D000894:Anti-Inflammatory Agents, Non-Steroidal; D013723:Teratogens; D000431:Ethanol; D001241:Aspirin