Migration behavior of isoluminol isothiocyanate-labeled alpha-amino acids in capillary electrophoresis with an absorption/chemiluminescence dual detection system.
In our previous study, we developed capillary electrophoresis with an ultraviolet absorption/chemiluminescence dual detection system. Here, to demonstrate one of the possible applications of the capable system as well as confirm the advantage, migration behavior of isoluminol isothiocyanate-labeled alpha-amino acids was examined in the capillary electrophoresis with a dual detection system. The labeled samples were first analyzed by absorption detection with an on-capillary, followed by chemiluminescence detection with an end-capillary. The system easily, rapidly, and simultaneously produced useful information concerning chemiluminescence quenching and amino group-labeling due to the presence of both absorption and chemiluminescence detections.
Tsukagoshi
Kazuhiko
K
Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University, Kyotanabe 610-0321, Japan. ktsukago@mail.doshisha.ac.jp
Sawanoi
Kaori
K
Nakajima
Riichiro
R
D000596:Amino Acids / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D017879:Isothiocyanates / Q000737:chemistry*; D049449:Luminescence; D008165:Luminol / Q000031:analogs & derivatives* / Q000737:chemistry
D000596:Amino Acids; D017879:Isothiocyanates; C032487:isoluminol; D008165:Luminol