Quantification of leukotriene B4 glucuronide in human urine.
We have developed a method for measuring leukotriene B4 glucuronide, a marker of systemic leukotriene B4 biosynthesis, in human urine. This method involves the separation of two positional isomers of leukotriene B4 glucuronide by high-performance liquid chromatography, followed by hydrolysis with beta-glucuronidase and then leukotriene B4 quantification by enzyme immunoassay after purification by high-performance liquid chromatography. One of two positional isomers of leukotriene B4 glucuronide was predominantly present in urine. The concentration of the isomer increased in urine from aspirin-intolerant asthma patients after aspirin challenge. Urinary leukotriene E4 and leukotriene B4 glucuronide concentrations in 13 normal healthy adults were 94.6 pg/mg-creatinine (median) and 22.3 pg/mg-creatinine, respectively. Urinary LTE4 concentration increased during the first 3h after allergen inhalation in atopic patients. However, allergen-induced bronchoconstriction was not associated with an increased concentration of LTB4 glucuronide in urine. The method enabled us to precisely determine urinary leukotriene B4 glucuronide concentration.
Mita
Haruhisa
H
Clinical Research Center, Sagamihara National Hospital, 18-1 Sakuradai, Sagamihara, Kanagawa 228-8522, Japan. h-mita@sagamihara-hosp.gr.jp
Turikisawa
Naomi
N
Yamada
Tetsuo
T
Taniguchi
Masami
M
D000328:Adult; D000368:Aged; D001249:Asthma / Q000652:urine; D001760:Bleeding Time; D001985:Bronchial Provocation Tests; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005966:Glucuronidase / Q000378:metabolism; D020719:Glucuronides / Q000737:chemistry / Q000652:urine*; D006801:Humans; D006868:Hydrolysis; D007124:Immunoenzyme Techniques; D007536:Isomerism; D007700:Kinetics; D007975:Leukotriene B4 / Q000031:analogs & derivatives* / Q000737:chemistry / Q000652:urine; D017999:Leukotriene E4 / Q000652:urine; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D008875:Middle Aged; D013997:Time Factors
D020719:Glucuronides; C494103:leukotriene B4 glucuronide; D007975:Leukotriene B4; D017999:Leukotriene E4; D005966:Glucuronidase