title: Expression of asparagine synthetase genes in sunflower (Helianthus annuus) under various environmental stresses.
abstract: In sunflower, asparagine synthetase (AS; EC 6.3.5.4) is encoded by a small family of three genes (HAS1, HAS1.1 and HAS2) that are differentially regulated by light, carbon and nitrogen availability. In this study, the response of each gene to various stress conditions was examined by Northern analysis with gene-specific probes in leaves and roots. The expression of HAS1 and HAS1.1 genes was induced by osmotic stress (300 mM mannitol), salt stress (150 mM NaCl), and heavy-metal stress (20 microM CuSO(4)), more in roots than in leaves. The expression of HAS2 was not significantly altered by stress treatments. The positive response of HAS1 and HAS1.1 genes to osmotic and salt stresses occurred in the light, in contrast to that previously found in unstressed plants. Measurements of sucrose and total free amino acid contents in leaves and roots indicate that the expression of root HAS1 and HAS1.1 genes in stressed plants is not under metabolic control by the intracellular C/N ratio, suggesting the involvement of some specific stress factor(s). Growth of plants at 40 degrees C for 12h negatively affected the expression of HAS1 and HAS1.1 but not that of HAS2.
authors.affiliation: Departamento de Fisiología, Anatomía y Biología Celular, Area de Fisiología Vegetal, Facultad de Ciencias Experimentales, Universidad Pablo de Olavide, Ctra. de Utrera. km 1, 41013 Sevilla, Spain.
authors.forename: María Begoña
authors.initials: MB
authors.lastname: Herrera-Rodríguez
authors.forename: Rafael
authors.initials: R
authors.lastname: Pérez-Vicente
authors.forename: José-María
authors.initials: JM
authors.lastname: Maldonado
mesh_terms: D001217:Aspartate-Ammonia Ligase / Q000235:genetics*; D002244:Carbon / Q000378:metabolism; D004777:Environment; D006368:Helianthus / Q000201:enzymology / Q000235:genetics*; D008027:Light; D019216:Metals, Heavy / Q000633:toxicity; D009584:Nitrogen / Q000378:metabolism; D018515:Plant Leaves / Q000201:enzymology; D018517:Plant Roots / Q000201:enzymology
chemical_list: D019216:Metals, Heavy; D002244:Carbon; D001217:Aspartate-Ammonia Ligase; D009584:Nitrogen