title: Multiple forms of maize endosperm adp-glucose pyrophosphorylase and their control by shrunken-2 and brittle-2.
abstract: Heat-labile and heat stable forms of ADP-glucose pyrophosphorylase were identified in the maize endosperm. The heat-labile form is destroyed by normal electrophoretic conditions. The heat-stable form corresponds to pyrophosphorylase B. In wild type, 96% of the total activity is heat labile. Both forms are reduced in 11 brittle-2 (bt2) and 12 shrunken-2 (sh2) mutants. The heat-labile form is reduced to a greater extent than is the heat-stable form in each of the 23 mutants. Deletion of sh2 abolishes both forms. The original ratio of the two forms is restored after sh2 function is expressed via transposition of Dissociation from sh2. The possible roles of these genes in the control of ADP-glucose pyrophosphorylase are discussed.
authors.affiliation: Vegetable Crops Department, Department of Microbiology and Cell Science, University of Florida, Gainesville, Florida 32611.
authors.forename: L C
authors.initials: LC
authors.lastname: Hannah
authors.forename: D M
authors.initials: DM
authors.lastname: Tuschall
authors.forename: R J
authors.initials: RJ
authors.lastname: Mans