Bolevenine, a toxic protein from the Japanese toadstool Boletus venenatus.
A toxic protein, called bolevenine, was isolated from the toxic mushroom Boletus venenatus based on its lethal effects on mice. On SDS-PAGE, in either the presence or absence of 2-mercaptoethanol, this protein showed a single band of approximately 12 kDa. In contrast, based on gel filtration and MALDI-TOFMS, its relative molecular mass was estimated to be approximately 30 kDa and approximately 33 kDa, respectively, indicating that the protein consists of three identical subunits. This toxin exhibited its lethal activity following injection at 10mg/kg into mice. The N-terminal amino acid sequence was determined up to 18, and found to be similar to the previously reported bolesatine, a toxic compound isolated from Boletus satanas.
Matsuura
Masanori
M
Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan.
Yamada
Mina
M
Saikawa
Yoko
Y
Miyairi
Kazuo
K
Okuno
Toshikatsu
T
Konno
Katsuhiro
K
Uenishi
Jun'ichi
J
Hashimoto
Kimiko
K
Nakata
Masaya
M
D000363:Agaricales / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005656:Fungal Proteins / Q000302:isolation & purification / Q000378:metabolism / Q000633:toxicity*; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D051379:Mice; D008969:Molecular Sequence Data; D009183:Mycotoxins / Q000302:isolation & purification / Q000378:metabolism / Q000633:toxicity*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D018675:Toxicity Tests / Q000379:methods
D005656:Fungal Proteins; D009183:Mycotoxins; C060924:Bolesatine protein, Boletus satanas