title: Bolevenine, a toxic protein from the Japanese toadstool Boletus venenatus.
abstract: A toxic protein, called bolevenine, was isolated from the toxic mushroom Boletus venenatus based on its lethal effects on mice. On SDS-PAGE, in either the presence or absence of 2-mercaptoethanol, this protein showed a single band of approximately 12 kDa. In contrast, based on gel filtration and MALDI-TOFMS, its relative molecular mass was estimated to be approximately 30 kDa and approximately 33 kDa, respectively, indicating that the protein consists of three identical subunits. This toxin exhibited its lethal activity following injection at 10mg/kg into mice. The N-terminal amino acid sequence was determined up to 18, and found to be similar to the previously reported bolesatine, a toxic compound isolated from Boletus satanas.
authors.affiliation: Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Matsuura
authors.forename: Mina
authors.initials: M
authors.lastname: Yamada
authors.forename: Yoko
authors.initials: Y
authors.lastname: Saikawa
authors.forename: Kazuo
authors.initials: K
authors.lastname: Miyairi
authors.forename: Toshikatsu
authors.initials: T
authors.lastname: Okuno
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Konno
authors.forename: Jun'ichi
authors.initials: J
authors.lastname: Uenishi
authors.forename: Kimiko
authors.initials: K
authors.lastname: Hashimoto
authors.forename: Masaya
authors.initials: M
authors.lastname: Nakata
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005656:Fungal Proteins / Q000302:isolation & purification / Q000378:metabolism / Q000633:toxicity*; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D051379:Mice; D008969:Molecular Sequence Data; D009183:Mycotoxins / Q000302:isolation & purification / Q000378:metabolism / Q000633:toxicity*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D018675:Toxicity Tests / Q000379:methods
chemical_list: D005656:Fungal Proteins; D009183:Mycotoxins; C060924:Bolesatine protein, Boletus satanas