title: Deposition of bone-like hydroxyapatite on the surface of silk cloth with the aid of immobilized urease.
abstract: Continuous layers of hydroxyapatite were deposited on silk cloth from aqueous solutions by using urease as the precipitant supplier. Silk cloth was surface-modified with urease and was immersed in an aqueous solution containing Ca2+, PO4(3-) , and urea. As urea was hydrolyzed to form ammonia with the aid of the immobilized urease, hydroxyapatite precipitated predominantly on the surface of the silk cloth. It took only a few hours to form continuous layers of hydroxyapatite on the silk cloth. The resultant hydroxyapatite was found to be bone-like apatite because it had low crystallinity, contained carbonate ion in the lattice, and had a calcium-deficient composition.
authors.affiliation: Department of Chemistry and Chemical Engineering, Faculty of Engineering, Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata 992-8510, Japan. unuma@yz.yamagata-u.ac.jp
authors.forename: Hidero
authors.initials: H
authors.lastname: Unuma
authors.forename: Makiko
authors.initials: M
authors.lastname: Hiroya
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ito
mesh_terms: D018786:Bone Substitutes / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D047011:Silk / Q000737:chemistry*; D012996:Solutions; D013499:Surface Properties; D014508:Urea / Q000737:chemistry; D014510:Urease / Q000737:chemistry* / Q000378:metabolism
chemical_list: D018786:Bone Substitutes; D004800:Enzymes, Immobilized; D047011:Silk; D012996:Solutions; D014508:Urea; D017886:Durapatite; D014510:Urease