title: Modifications of Phleum pratense grass pollen allergens following artificial exposure to gaseous air pollutants (O(3), NO(2), SO(2)).
abstract: BACKGROUND
  Air pollution is frequently proposed as a potential cause of the increased incidence of allergy in industrialised countries. Our objective was to investigate the impact of the major gaseous air pollutants on grass pollen allergens.
  
  
  METHODS
  Timothy grass pollen was exposed to ozone (O(3)), nitrogen dioxide (NO(2)) and sulphur dioxide (SO(2)) alone or in combination. Allergen contents were analysed by 2-dimensional immunoblot using grass pollen-sensitive patient sera.
  
  
  RESULTS
  For O(3)-treated pollen, immunoblotting showed an acidification of allergens Phl p 1b, Phl p 4, Phl p 5 and Phl p 6 and an IgE recognition decrease in Phl p 1, Phl p 2, Phl p 6 and Phl p 13. NO(2) exposure induced a decrease in Phl p 2, Phl p 5b and Phl p 6 recognition, and SO(2) treatment induced a decrease in Phl p 2, Phl p 6 and Phl p 13 recognition. Moreover, samples treated with a mix of NO(2)/O(3) or NO(2)/SO(2) showed a higher decrease in allergen content, compared with samples treated with only one pollutant. The O(3) acidification was also observed with the NO(2)/O(3) mix.
  
  
  CONCLUSIONS
  Exposure of pollen to gaseous pollutants induced a decrease in allergen detection in pollen extracts. This decrease could be due to a mechanical loss of allergens from the altered pollen grains and/or post-translational modifications affecting allergen recognition by IgE.
authors.affiliation: Institut National de l'Environnement Industriel et des Risques, Verneuil-en-Halatte, France. francoise.rogerieux@ineris.fr
authors.forename: F
authors.initials: F
authors.lastname: Rogerieux
authors.forename: D
authors.initials: D
authors.lastname: Godfrin
authors.forename: H
authors.initials: H
authors.lastname: Sénéchal
authors.forename: A C
authors.initials: AC
authors.lastname: Motta
authors.forename: M
authors.initials: M
authors.lastname: Marlière
authors.forename: G
authors.initials: G
authors.lastname: Peltre
authors.forename: G
authors.initials: G
authors.lastname: Lacroix
mesh_terms: D000393:Air Pollutants / Q000737:chemistry*; D052179:Antigens, Plant / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology; D015151:Immunoblotting; D009585:Nitrogen Dioxide / Q000737:chemistry; D010126:Ozone / Q000737:chemistry; D031784:Phleum / Q000737:chemistry*; D011058:Pollen / Q000737:chemistry*; D013458:Sulfur Dioxide / Q000737:chemistry
chemical_list: D000393:Air Pollutants; D052179:Antigens, Plant; D013458:Sulfur Dioxide; D010126:Ozone; D009585:Nitrogen Dioxide