title: Aberrant termination of reproduction-related TMEM30C transcripts in the hominoids.
abstract: Finding genetic novelties that may contribute to human-specific physiology and diseases is a key issue of current biomedical studies. TMEM30C is a gene containing two transmembrane (TM) domains and homologous to the yeast CDC50 family, which is related to polarized cell division. It is conserved among mammals along with two other paralogs, TMEM30A and TMEM30B. We found that TMEM30C is expressed specifically in the testis of mammals, in contrast to the relatively wide expression distributions of the other paralogs. While macaques expressed two alternative splicing isoforms which include one or two TM domains, humans and chimpanzees predominantly expressed truncated transcripts because of the mutations in the splicing and/or poly(A) signal sites. The major transcript in humans harbored non-stop ORF (open reading frame) while the chimpanzee counterpart encoded a protein with one TM domain. The difference was due to the 1-bp indel upstream of the poly(A) signal site. In addition, both the hominoids expressed minor transcripts encoding short proteins with one TM domain. Phylogenetic analysis has showed the acceleration of amino acid substitution after the human and chimpanzee divergence, which may have been caused by a recent relaxation in functional constraints or positive selection on TMEM30C. Elucidating the precise reproductive function of TMEM30C in mammals will be important to the foundation of divergence in higher primates at a molecular level.
authors.affiliation: Division of Biomedical Research Resources, National Institute of Biomedical Innovation, 7-6-8 Saito-Asagi, Ibaraki, Osaka 567-0085, Japan. nosada@nibio.go.jp
authors.forename: Naoki
authors.initials: N
authors.lastname: Osada
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Hashimoto
authors.forename: Momoki
authors.initials: M
authors.lastname: Hirai
authors.forename: Jun
authors.initials: J
authors.lastname: Kusuda
mesh_terms: D000818:Animals; D001483:Base Sequence; D019143:Evolution, Molecular; D020869:Gene Expression Profiling; D008040:Genetic Linkage; D015186:Hominidae / Q000235:genetics* / Q000502:physiology*; D006801:Humans; D007246:Infertility / Q000235:genetics*; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D002679:Pan troglodytes / Q000235:genetics; D010802:Phylogeny; D012333:RNA, Messenger / Q000032:analysis; D012098:Reproduction / Q000235:genetics*
chemical_list: D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D012333:RNA, Messenger; C520097:TMEM30CP protein, human