Campylobacter coli isolates derived from chickens in Senegal: Diversity, genetic exchange with Campylobacter jejuni and quinolone resistance.
We used the multilocus sequence typing (MLST) method to study the genetic diversity of Campylobacter coli isolated from chickens in Senegal, and to check the presence of genetic exchange with Campylobacter jejuni. In addition, we assessed the resistance of the isolates to ciprofloxacin and nalidixic acid, and their gyrA sequences. MLST revealed a low level of diversity and the absence of lineages among C. coli isolates. In addition, an exchange of alleles with C. jejuni was found. Twenty percent of the ciprofloxacin-resistant isolates lacked mutations within the quinolone resistance-determining region (QRDR) of GyrA. There was no link between quinolone resistance and sequence type (ST).
Kinana
Alfred Dieudonné
AD
Laboratoire de Biologie médicale, Institut Pasteur de Dakar, 36, avenue Pasteur, Dakar, Sénégal. kinana@pasteur.sn
Cardinale
Eric
E
Bahsoun
Ibrahim
I
Tall
Fatou
F
Sire
Jean-Marie
JM
Breurec
Sebastien
S
Garin
Benoit
B
Saad-Bouh Boye
Cheikh
C
Perrier-Gros-Claude
Jean-David
JD
D000483:Alleles; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D017000:Campylobacter coli / Q000187:drug effects* / Q000235:genetics*; D016123:Campylobacter jejuni / Q000235:genetics; D002645:Chickens / Q000382:microbiology*; D002939:Ciprofloxacin / Q000494:pharmacology; D027081:DNA Gyrase / Q000235:genetics; D024881:Drug Resistance, Bacterial; D014644:Genetic Variation; D009268:Nalidixic Acid / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D015363:Quinolones / Q000494:pharmacology*; D012675:Senegal
D000890:Anti-Infective Agents; D015363:Quinolones; D009268:Nalidixic Acid; D002939:Ciprofloxacin; D027081:DNA Gyrase