Mutation of rpoS enhances Pseudomonas sp. KL28 growth at higher concentrations of m-cresol and changes its surface-related phenotypes.
A Tn5 transposon mutant was isolated of the alkylphenol degrader Pseudomonas sp. KL28 that showed increased growth at higher levels of m-cresol on solid and in liquid cultures. The transposon was inserted at the 5'-terminus of rpoS, which encodes a stationary-phase sigma factor. When grown on agar plates, the rpoS mutant developed prominent wrinkles, especially at lower temperatures, and spread faster on soft agar. In addition, the rpoS mutant had enhanced biofilm-forming ability that was not due to self-produced diffusible signals.
Yun
Ji In
JI
Department of Microbiology, Changwon National University, Changwon-si, Kyongnam, Korea.
Cho
Kyoung Mi
KM
Kim
Jin-Kyoo
JK
Lee
Soo O
SO
Cho
Kyungyun
K
Lee
Kyoung
K
D000362:Agar; D001426:Bacterial Proteins / Q000235:genetics*; D001431:Bacteriological Techniques; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D002876:Chromosomes, Bacterial; D003408:Cresols / Q000494:pharmacology*; D004251:DNA Transposable Elements; D009154:Mutation; D010641:Phenotype; D011549:Pseudomonas / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D012808:Sigma Factor / Q000235:genetics*; D013696:Temperature
D001426:Bacterial Proteins; D003408:Cresols; D004251:DNA Transposable Elements; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria; C032538:4-cresol; D000362:Agar