Use of an inhibition enzyme-linked immunosorbent assay for quantification of capsular polysaccharide or proteins in vaccines.
An inhibition enzyme-linked immunosorbent assay (ELISA) is described for quantification of capsular polysaccharide or proteins in vaccines and other samples containing whole cells or extracts of Actinobacillus pleuropneumoniae. The assay can be used to quantify any antigen that can be purified and for which highly specific antibodies are not available. The assay can be carried out by any laboratory capable of performing an ELISA.
Inzana
Thomas J
TJ
Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061, USA. tinzana@vt.edu
Champion
Anna
A
D016977:Actinobacillus pleuropneumoniae / Q000276:immunology*; D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D016667:Bacterial Capsules / Q000032:analysis*; D001426:Bacterial Proteins / Q000032:analysis*; D001428:Bacterial Vaccines / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D011817:Rabbits
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines