Large scale preparation of the mammalian high mobility group protein A2 for biophysical studies.
Due to asymmetrical charge distribution of the mammalian high mobility group protein A2 (HMGA2), which makes HMGA2 bind to both cation- and anion-exchange columns, we developed a rapid procedure for purifying HMGA2 in the milligram range. This purification procedure greatly facilitated biophysical studies, which require large amounts of the protein.
Cui
Tengjiao
T
Department of Chemistry and Biochemistry, Florida International University, Miami, FL 33199, USA.
Joynt
Suzanne
S
Morillo
Victor
V
Baez
Maria
M
Hua
Zhichun
Z
Wang
Xiaotang
X
Leng
Fenfei
F
D000595:Amino Acid Sequence; D000818:Animals; D002852:Chromatography, Ion Exchange; D015870:Gene Expression; D025743:HMGA2 Protein / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*
D025743:HMGA2 Protein; D011994:Recombinant Proteins