Characterization of murine Dhx32.
In this report, we studied the expression of Dhx32, the murine counterpart of the human putative RNA helicase DHX32. Northern analysis showed widespread but variable level of expression among different tissues. RT-PCR detected the presence of two mRNA transcripts in several tissues that result from alternative usage of either exon 1 or exon 2 at the 5' UTR. Immunohistochemistry showed the presence of Dhx32 in all tissues examined including spleen, kidney and liver. The distribution of Dhx32 proteins was not uniform among different cell types within the same tissue, suggesting cell type-dependent regulation of expression. Finally, electron microscope immunocytochemistry detected the presence of Dhx32 in the mitochondria of hepatocytes.
Alli
Zaman
Z
Division of Haematopathology, Department of Paediatric Laboratory Medicine, Rm 3691, Atrium, Hospital for Sick Children, 555 University Avenue, Toronto, ON, Canada M5G 1X8.
Ho
Michael
M
Ackerley
Cameron
C
Abdelhaleem
Mohamed
M
D000818:Animals; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism*; D005091:Exons / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D051379:Mice; D016253:Microscopy, Immunoelectron; D008928:Mitochondria / Q000378:metabolism / Q000648:ultrastructure; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic
D012333:RNA, Messenger; D053487:DEAD-box RNA Helicases; C521665:Dhx32 protein, mouse