title: A new method for ABO genotyping to avoid discrepancy between genetic and serological determinations.
abstract: In Japan and elsewhere, ABO genotyping is frequently used in forensic practice for identification of a decomposed body. However, the phenotype deduced from the genotyping data is occasionally inconsistent with the real phenotype. In this paper, we report a simple ABO genotyping method in which five single nucleotide polymorphism at nps 220, 261, 796, 802, and 803 are analyzed simultaneously to avoid discrepancies between genetic and serological determinations in ABO*A204, *O303, *O207, *cis-AB01 and *cis-AB02 alleles. This method can be used for the genotyping of badly decomposed remains or old bloodstains.
authors.affiliation: Department of Forensic Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo, 113-0033, Japan. kaori@m.u-tokyo.ac.jp
authors.forename: Kaori
authors.initials: K
authors.lastname: Shintani-Ishida
authors.forename: Bao-Li
authors.initials: BL
authors.lastname: Zhu
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Maeda
authors.forename: Koichi
authors.initials: K
authors.lastname: Uemura
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Yoshida
mesh_terms: D000017:ABO Blood-Group System / Q000235:genetics*; D000483:Alleles; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
chemical_list: D000017:ABO Blood-Group System; D017931:DNA Primers