Solution structure of the twelfth cysteine-rich ligand-binding repeat in rat megalin.
Megalin, an approx. 600 kDa transmembrane glycoprotein that acts as multi-ligand transporter, is a member of the low density lipoprotein receptor gene family. Several cysteine-rich repeats, each consisting of about 40 residues, are responsible for the multispecific binding of ligands. The solution structure of the twelfth cysteine-rich ligand-binding repeat with class A motif found in megalin features two short beta-strands and two helical turns, yielding the typical fold with a I-III, II-V and IV-VI disulfide bridge connectivity pattern and a calcium coordination site at the C-terminal end. The resulting differences in electrostatic surface potential compared to other ligand-binding modules of this gene family, however, may be responsible for the functional divergence.
Wolf
Christian A
CA
Institute of Biophysical Chemistry, Center for Biomolecular Magnetic Resonance, J.W. Goethe-University of Frankfurt, 60439 Frankfurt, Germany.
Dancea
Felician
F
Shi
Meichen
M
Bade-Noskova
Veronika
V
Rüterjans
Heinz
H
Kerjaschki
Dontscho
D
Lücke
Christian
C
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D002118:Calcium / Q000737:chemistry; D003545:Cysteine / Q000737:chemistry*; D008024:Ligands; D026561:Low Density Lipoprotein Receptor-Related Protein-2 / Q000737:chemistry*; D008969:Molecular Sequence Data; D017510:Protein Folding; D017433:Protein Structure, Secondary; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid; D012996:Solutions / Q000737:chemistry; D055672:Static Electricity
D008024:Ligands; D026561:Low Density Lipoprotein Receptor-Related Protein-2; D012996:Solutions; D003545:Cysteine; D002118:Calcium