A comparison of promastigote and amastigote antigens of Leishmania infantum in the diagnosis of visceral Leishmaniosis by ELISA and IFA tests in Turkey.
This study was designed to evaluate the ELISA and IFA tests for diagnosis of visceral leishmaniosis (VL) using soluble and crude antigens from promastigote and amastigote forms of Leishmania infantum. A total of 70 sera were tested, including 30 patients who were suspected for Leishmaniosis (10 healthy persons, 10 patients with Toxoplasmosis, 10 patients with Giardiosis). In the 30 patients suspected to have Leishmaniosis, smears were prepared from bone marrow aspirates and all material aspirated was cultured in NNN medium. The results were compared with those of parasitological and serological tests. While 2 patients were serologically positive and parasitologically negative from the 30 patients, 8 patients were serologically and parasitologically positive. The amastigote and promastigote antigens were 100% specific. Using these antigens with ELISA and IFA tests did not result and diagnosis different than VL.
Altintaş
N
N
Ege University Medical Faculty, Department of Parasitology, Izmir, Turkey.
Ozbel
Y
Y
D000818:Animals; D000941:Antigens / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D007231:Infant, Newborn; D018314:Leishmania infantum / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000175:diagnosis* / Q000469:parasitology; D014421:Turkey
D000941:Antigens