title: Indirect immunofluorescent assay for antinuclear antibodies on McCoy-Plovdiv serum-free cell line substrate.
abstract: Indirect immunofluorescent assay for antinuclear antibodies (IIFA-ANA) on HEp-2 cell substrate is a widely used test for diagnosis of connective tissue diseases. Recently, serum-free fibroblast cell line McCoy-Plovdiv has been developed to provide an alternative substrate. The aim of the present study was to evaluate the diagnostic performance of IIFA-ANA on McCoy-Plovdiv cell substrate for systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) and compare it with that of the standard HEp-2 cell substrate. Sera from 72 patients with RA and 23 patients with SLE were tested with IIFA-ANA on both substrates. The control group consisted of 100 sera from healthy individuals. The agreement (Cohen's kappa) and the diagnostic performance (ROC analysis) of both methods were evaluated. IIFA-ANA on McCoy-Plovdiv cells proved to be comparable with the standard IIFA-ANA on HEp-2 cell substrate.
authors.affiliation: Department of Microbiology and Immunology, Medical University-Plovdiv, 15A Vassil Aprilov Street, 4000 Plovdiv, Bulgaria.
authors.forename: Marin Yordanov
authors.initials: MY
authors.lastname: Zagorov
authors.forename: Marian Marinov
authors.initials: MM
authors.lastname: Draganov
authors.forename: Stanislava Aleksandrova
authors.initials: SA
authors.lastname: Alimanska
authors.forename: Nedyalka Doncheva
authors.initials: ND
authors.lastname: Staykova
authors.forename: Rumen Stefanov
authors.initials: RS
authors.lastname: Stefanov
authors.forename: Monica
authors.initials: M
authors.lastname: Trayancheva
authors.forename: Stefka Ivanova
authors.initials: SI
authors.lastname: Kuzmanova
authors.forename: Marianna Atanasova
authors.initials: MA
authors.lastname: Murdjeva
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000974:Antibodies, Antinuclear / Q000097:blood*; D001172:Arthritis, Rheumatoid / Q000175:diagnosis*; D016022:Case-Control Studies; D002460:Cell Line; D005260:Female; D005347:Fibroblasts; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity
chemical_list: D000974:Antibodies, Antinuclear