In vitro Propagation of Valeriana wallichii.
A tissue culture procedure has been developed for the rapid multiplication of VALERIANA WALLICHII D C. through shoot tip and axillary bud explants. MS medium containing Kn or BAP (5.0 mg/l (-1)) in combination with IAA (1.0 mg/l (-1)) induced an optimal growth of shoots within 6-8 days from both apical and axillary bud explants. The roots developed on the same medium within 2-3 weeks. Hardening of IN VITRO grown plantlets in pots under glass-house conditions was dependent upon the temperature and humidity. A cold-temperate climate favoured early establishment. Following the given procedure, a large number of plants have been established under field conditions at two locations. The method has implications in the early introduction of an elite population as well as its improvement.
Mathur
J
J
Division of Plant Tissue Culture, Central Institute of Medicinal and Aromatic Plants (CIMAP), P.B. No. 1, R. S. M. Nagar, Lucknow-226016, India.
Ahuja
P S
PS
Mathur
A
A
Kukreja
A K
AK
Shah
N C
NC