title: Diversity of arsenite transporter genes from arsenic-resistant soil bacteria.
abstract: A PCR approach was developed to assess the occurrence and diversity of arsenite transporters in arsenic-resistant bacteria. For this purpose, three sets of degenerate primers were designed for the specific amplification of approximately 750bp fragments from arsB and two subsets of ACR3 (designated ACR3(1) and ACR3(2)) arsenite carrier gene families. These primers were used to screen a collection of 41 arsenic-resistant strains isolated from two soil samples with contrasting amounts of arsenic. PCR results showed that 70.7% of the isolates contained a gene related to arsB or ACR3, with three of them carrying both arsB and ACR3-like genes. Phylogenetic analysis of the protein sequences deduced from the amplicons indicated a prevalence of arsB in Firmicutes and Gammaproteobacteria, while ACR3(1) and ACR3(2) were mostly present in Actinobacteria and Alphaproteobacteria, respectively. In addition to validating the use of degenerate primers for the identification of arsenite transporter genes in a taxonomically wide range of bacteria, the study describes a novel collection of strains displaying interesting features of resistance to arsenate, arsenite and antimonite, and the ability to oxidize arsenite.
authors.affiliation: Laboratoire Ecotoxicité et Santé Environnementale (ESE), UMR7146, CNRS, Université Paul Verlaine, Campus Bridoux, rue du Général Delestraint, 57070 Metz, France. asma.rokbani@umail.univ-metz.fr
authors.forename: Asma Rokbani
authors.initials: AR
authors.lastname: Achour
authors.forename: Pascale
authors.initials: P
authors.lastname: Bauda
authors.forename: Patrick
authors.initials: P
authors.lastname: Billard
mesh_terms: D001151:Arsenic / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D017931:DNA Primers; D024881:Drug Resistance, Bacterial; D005798:Genes, Bacterial; D014644:Genetic Variation; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000235:genetics*; D006094:Gram-Positive Bacteria / Q000187:drug effects / Q000235:genetics*; D026901:Membrane Transport Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012988:Soil Microbiology
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D026901:Membrane Transport Proteins; D001151:Arsenic