title: Localization of lipids in the aortic wall with imaging TOF-SIMS.
abstract: Time-of-flight secondary-ion-mass-spectrometry (TOF-SIMS) was utilized to address the issue of localization of lipids and inorganic ions in healthy rat aorta and human atherosclerotic plaque. Pieces of rat aorta were high pressure frozen, freeze-fractured and freeze dried. The samples were analyzed by imaging TOF-SIMS equipped with a Bi(1-7)(+)-source. Reference lipid samples were analyzed and compared to data obtained by analysis of the rat aorta samples. Fatty acids, cholesterol, oxysterol and diacylglycerols were detected and localized. A heterogeneous lipid distribution could be shown in the aorta, where the lamellae of the aorta, distinguished by imaging of CN(-), appeared enriched in cholesterol, oxysterol and diacylglycerols, while the smooth muscle tissue, identified by imaging of PO(3), appeared enriched in phosphocholine. Palmitic/palmitoleic acid and stearic/oleic acid appeared to be heterogeneously distributed over the aorta with high concentration areas located especially in the tunica media region of the aorta. Human atherosclerotic plaque showed an irregular cholesterol distribution mainly located in spots in the intima region with elongated diacylglycerol regions located mainly in the media region.
authors.affiliation: Department of Anatomy and Cell Biology, Göteborg University, P.O. Box 420, SE - 405 30 Göteborg, Sweden.
authors.forename: Per
authors.initials: P
authors.lastname: Malmberg
authors.forename: Katrin
authors.initials: K
authors.lastname: Börner
authors.forename: Yun
authors.initials: Y
authors.lastname: Chen
authors.forename: Peter
authors.initials: P
authors.lastname: Friberg
authors.forename: Birgit
authors.initials: B
authors.lastname: Hagenhoff
authors.forename: Jan-Erik
authors.initials: JE
authors.lastname: Månsson
authors.forename: Håkan
authors.initials: H
authors.lastname: Nygren
mesh_terms: D000818:Animals; D001011:Aorta / Q000378:metabolism* / Q000648:ultrastructure; D050197:Atherosclerosis / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D005229:Fatty Acids, Monounsaturated / Q000378:metabolism; D005612:Freeze Drying; D006801:Humans; D008055:Lipids / Q000032:analysis*; D019301:Oleic Acid / Q000378:metabolism; D019308:Palmitic Acid / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D018629:Spectrometry, Mass, Secondary Ion / Q000379:methods*; D013229:Stearic Acids / Q000378:metabolism
chemical_list: D005229:Fatty Acids, Monounsaturated; D008055:Lipids; D013229:Stearic Acids; C008757:palmitoleic acid; D019301:Oleic Acid; D019308:Palmitic Acid; C031183:stearic acid; D002784:Cholesterol