Stress-induced enhancement of activity of lymphocyte lysosomal enzymes in pigs of different stress-susceptibility.
To evaluate a possible mechanism of stress-induced lymphopenic effect we assessed the activity of lymphocyte lysosomal enzymes (LE) under immobilization. The effects of immobilization stress on LE (AP, acid phosphatase, cathepsin D and L, beta-N-acetyl-glucosamidase) activity in lymphocytes, number of lymphocytes and plasma cortisol (COR) level in the peripheral blood were examined in the cross-bred Pietrain pigs showing genotypic (presence or lack of RyR1 gene mutation) and phenotypic (reactivity to halothane) differences. It was found that immobilization stress evoked an increase in LE which was concomitant with lymphopenia and a rise of COR level. The most pronounced enhancement of LE, which may reflect a tendency to lymphocyte cytolysis, was found in the recessive homozygotes RyR1 (nn) phenotypically defined as stress/halothane susceptible as well as in the heterozygotes RyR1 (Nn) included in the group of stress/halothane resistant. Despite this individual variability the stress-induced increase in LE activity was present in all the animals. It seems that a possibility of destruction (lysis) of lymphocyte cells should not be excluded as one of the causes of stress lymphopenia.
Stojek
W
W
Department of Animal Physiology, Institute of Biology, University of Gdańsk, Gdańsk, Poland.
Borman
A
A
Glac
W
W
Baracz-Jóźwik
B
B
Witek
B
B
Kamyczek
M
M
Tokarski
J
J
D000118:Acetylglucosaminidase / Q000097:blood; D000135:Acid Phosphatase / Q000097:blood; D000818:Animals; D002402:Cathepsin D / Q000097:blood; D056668:Cathepsin L; D002403:Cathepsins / Q000097:blood; D003546:Cysteine Endopeptidases / Q000097:blood; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006854:Hydrocortisone / Q000097:blood; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000201:enzymology*; D008231:Lymphopenia / Q000201:enzymology / Q000209:etiology; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D012149:Restraint, Physical; D013312:Stress, Physiological / Q000097:blood / Q000201:enzymology* / Q000209:etiology / Q000235:genetics; D013552:Swine / Q000097:blood* / Q000235:genetics
D000135:Acid Phosphatase; D000118:Acetylglucosaminidase; D002403:Cathepsins; D003546:Cysteine Endopeptidases; D056668:Cathepsin L; D002402:Cathepsin D; D006854:Hydrocortisone