Construction and transplantation of an engineered hepatic tissue using a polyaminourethane-coated nonwoven polytetrafluoroethylene fabric.
BACKGROUND
Acute liver failure (ALF) is a serious condition that has a high mortality rate. Construction of an efficient culture and transplantation engineering system of hepatic tissue is an important approach to treat patients suffering from ALF to provide short-term hepatic support until the damaged liver spontaneously recovers or a donor liver becomes available for transplantation. Here, we evaluate the construction and transplantation of an engineered hepatic tissue (EHT) using primary isolated hepatocytes cultured onto polyaminourethane (PAU)-coated, nonwoven polytetrafluoroethylene (PTFE) fabric.


METHODS
The isolated hepatocytes cultured onto PAU-coated PTFE fabric were able to adhere and spread over the individual fibers of the net and formed hepatic clusters after 3 days, such clusters revealed Gap junctions and well-developed bile canaliculi.


RESULTS
When PAU-coated PTFE was utilized, ammonia-, and diazepam- metabolizing capacities and albumin production ability were significantly increased compared with collagen control. To test the function of this hepatic tissue in vivo, we transplanted a nonwoven PAU-coated PTFE fabric inoculated with one million hepatocytes on the surface of the spleen of Balb/c mice suffering from ALF induced by 90% hepatectomy, and found that this EHT prolonged the survival of liver failure-induced mice without adverse effects. Ultrastructure analyses showed good attachment of the cells on the surface of PTFE fabric and strong albumin expression seven days after the newly formed hepatic tissue was transplanted.


CONCLUSIONS
We have here demonstrated the efficient construction and transplantation of hepatic tissue using primary hepatocytes and PAU-coated PTFE fabric.
Soto-Gutierrez
Alejandro
A
Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan.
Navarro-Alvarez
Nalu
N
Rivas-Carrillo
Jorge D
JD
Tanaka
Kimiaki
K
Chen
Yong
Y
Misawa
Haruo
H
Okitsu
Teru
T
Noguchi
Hirofumi
H
Tanaka
Noriaki
N
Kobayashi
Naoya
N
D000418:Albumins / Q000096:biosynthesis; D000641:Ammonia / Q000378:metabolism; D000818:Animals; D002470:Cell Survival; D003975:Diazepam / Q000378:metabolism; D006498:Hepatectomy; D006801:Humans; D008099:Liver / Q000166:cytology / Q000378:metabolism / Q000601:surgery; D016031:Liver Transplantation / Q000379:methods*; D019164:Liver, Artificial; D051379:Mice; D046529:Microscopy, Electron, Transmission; D011138:Polytetrafluoroethylene; D011140:Polyurethanes; D015996:Survival Rate; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*
D000418:Albumins; D011140:Polyurethanes; D000641:Ammonia; D011138:Polytetrafluoroethylene; D003975:Diazepam