title: Characterization of the genetic background of Vibrio cholerae O1 biotype El Tor serotype Inaba strains isolated in Trivandrum, southern India.
abstract: Isolates of Vibrio cholerae O1 biotype El Tor serotype Inaba associated with an outbreak of cholera in Trivandrum, southern India, were characterized. PCR testing revealed that all five isolates examined carried the TCP pathogenicity island, the CTX genetic element and the RTX toxin, and produced cholera toxin (CT). RFLP analysis revealed that these Inaba isolates possessed a single copy of the CTX element flanked by two tandemly arranged copies of the RS element upstream of the core region. The isolates were resistant to ampicillin, nalidixic acid, trimethoprim, sulfamethoxazole, streptomycin and the vibriostatic agent 2,4-diamino-6,7-diisopropylpteridine (O/129). Ribotyping of these Inaba isolates revealed a hybridization profile similar to a strain of serotype Ogawa prevalent in southern India.
authors.affiliation: Institute of Life Sciences, Nalco Square, Bhubaneswar 751 023, India.
authors.forename: Saswat S
authors.initials: SS
authors.lastname: Mohapatra
authors.affiliation: Rajiv Gandhi Centre for Biotechnology, Jagathy, Trivandrum 695 014, India.
authors.forename: Dhanya
authors.initials: D
authors.lastname: Ramachandran
authors.affiliation: Institute of Life Sciences, Nalco Square, Bhubaneswar 751 023, India.
authors.forename: Chinmay K
authors.initials: CK
authors.lastname: Mantri
authors.affiliation: Institute of Life Sciences, Nalco Square, Bhubaneswar 751 023, India.
authors.forename: Durg V
authors.initials: DV
authors.lastname: Singh
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002771:Cholera / Q000453:epidemiology / Q000382:microbiology*; D002772:Cholera Toxin / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004196:Disease Outbreaks; D024881:Drug Resistance, Bacterial; D018628:Gene Dosage; D005798:Genes, Bacterial; D044404:Genomic Islands / Q000235:genetics; D006801:Humans; D007194:India / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D021521:Ribotyping; D012703:Serotyping; D041241:Vibrio cholerae O1 / Q000145:classification* / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D002772:Cholera Toxin