Two-dimensional versus three-dimensional morphometry of monogenoidean sclerites.
A new method of three-dimensional (3-D) analysis of sclerotised structures of monogenoids was performed by processing z-series images using 3D-Doctor. Z-series were obtained from Gomori's trichrome-stained specimens of marine and freshwater monogenoids under laser scanning confocal fluorescence microscopy. Measurements obtained from 3-D images were then compared with those from 2-D images taken from both flattened and unflattened specimens. Data comparison demonstrated that 3-D morphometry allowed avoidance of over-estimation due to deformation and the reduction of errors associated with different spatial orientations. Moreover, study of 3-D images permitted observation of morphological details that are not detectable in 2-D representations.
Galli
Paolo
P
Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milano, Italy. paolo.galli@unimib.it
Strona
Giovanni
G
Villa
Anna Maria
AM
Benzoni
Francesca
F
Stefani
Fabrizio
F
Doglia
Silvia Maria
SM
Kritsky
Delane C
DC
D000818:Animals; D001699:Biometry / Q000379:methods; D005393:Fish Diseases / Q000469:parasitology*; D005399:Fishes / Q000469:parasitology; D006374:Helminthiasis, Animal / Q000469:parasitology*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D010986:Platyhelminths / Q000033:anatomy & histology*; D025341:Principal Component Analysis