Microbiologyc control in human heart valves.
OBJECTIVE
To evaluate, from microbiological point of view, the valves processed by Human Heart Valve Bank of Santa Casa de Misericórdia of Curitiba for use in cardiovascular surgeries.


METHODS
The processing of 1,671 valves, accomplished within the period of time between July 1999 and June 2004, was evaluated. Out of the valves and the solutions involved in the process, samples were collected and spread in culture mediums, such as fluid thioglycollate medium, tryptic soy broth and Sabouraud broth, for incubation during 14 days, using a modified methodology based on the Farmacopéia Brasileira 1988 (Brazilian Pharmacopeia) and USP 1990 (United States Pharmacopeia). The samples in which growing was observed were submitted to microbian identification.


RESULTS
In a set of 1,671 samples, 92% were considered proper for use under microbiological point of view, since they did not display microbian contamination. The remaining 8% were rejected for clinical use because of contamination in some stage of the valve processing.


CONCLUSIONS
From the Analysis of the results, it was observed the importance of microbiological control in human grafts, in order to avoid using microbiologically contaminated valves in patients submitted to cardiovascular surgery.
Peruzzo
Angela Maria
AM
Cardioprótese--Ind. e Com. de Produtos para Saúde, Pontifícia Universidade Católica do Paraná, Universidade Federal do Paraná, Passo Fundo, RS, Brazil. angelamariaperuzzo@yahoo.com.br
da Costa
Francisco Diniz Affonso
FD
Abrahão
Wanda Moscalewski
WM
D015925:Cryopreservation / Q000592:standards*; D006351:Heart Valves / Q000382:microbiology* / Q000637:transplantation*; D006801:Humans; D014015:Tissue Banks / Q000592:standards*