title: Haloferax prahovense sp. nov., an extremely halophilic archaeon isolated from a Romanian salt lake.
abstract: A novel halophilic archaeon, strain TL6T, was isolated from Telega Lake, a hypersaline environment in Prahova county, Romania. Strain TL6T was able to grow in media with a salt concentration of between 2.5 and 5.2 M, with optimum growth at a concentration of 3.5 M. The novel strain was able to grow at concentrations of 1 M MgCl2 or less, with an optimum of 0.4 M Mg2+. Growth of the novel strain occurred between pH 6.0 and 8.5, with an optimum of pH 7.0-7.5. The G+C content of the total DNA was 63.7 mol%. The 16S rRNA gene sequence of the novel strain was most closely related to species of the genus Haloferax (97.3-99.3 % sequence similarity). The lipid profile of the novel strain corresponded to that of other species belonging to the genus Haloferax. A comparative analysis of the phenotypic properties and DNA-DNA hybridization between the novel strain and other species of the genus Haloferax strongly supported the conclusion that strain TL6T represents a novel species within this genus, for which the name Haloferax prahovense sp. nov., is proposed. The type strain is TL6T (=JCM 13924T=DSM 18310T).
authors.affiliation: Institute of Biology of the Romanian Academy, Splaiul Independentei 296, PO Box 56-53, Bucharest 060031, Romania.|Japan Collection of Micro-organisms RIKEN BioResource Center, Wako-shi, Saitama-ken 351-0198, Japan.
authors.forename: Madalin
authors.initials: M
authors.lastname: Enache
authors.affiliation: Japan Collection of Micro-organisms RIKEN BioResource Center, Wako-shi, Saitama-ken 351-0198, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Itoh
authors.affiliation: Halophiles Research Institute, 677-1 Shimizu, Noda-shi, Chiba-ken 278-0043, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kamekura
authors.affiliation: Institute of Biology of the Romanian Academy, Splaiul Independentei 296, PO Box 56-53, Bucharest 060031, Romania.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Teodosiu
authors.affiliation: Institute of Biology of the Romanian Academy, Splaiul Independentei 296, PO Box 56-53, Bucharest 060031, Romania.
authors.forename: Lucia
authors.initials: L
authors.lastname: Dumitru
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D050260:Carbohydrate Metabolism; D019641:DNA, Archaeal / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D020459:Genes, rRNA; D019621:Haloferax / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000032:analysis; D015636:Magnesium Chloride; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D019642:RNA, Archaeal / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012383:Romania; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D013696:Temperature; D014871:Water Microbiology
chemical_list: D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D008055:Lipids; D019642:RNA, Archaeal; D012336:RNA, Ribosomal, 16S; D015636:Magnesium Chloride; D012965:Sodium Chloride