Antiinflammatory effect of topically applied propolis extract in carrageenan-induced rat hind paw edema.
The antiinflammatory effect of an ointment containing propolis extract (3%-7%) was examined using carrageenan-induced hind paw edema in rats. Treatment with the ointment inhibited the edema moderately, and the inhibition was significant at 5% and 7%. Additionally, the effect of the ointment on chemotaxis of human polymorphonuclear leukocytes (PMNs) was investigated using the agarose plate method. Migration of PMNs toward zymosan-treated serum was inhibited in the presence of 5% propolis ointment. These results demonstrate that topical application of propolis extract is effective in inhibiting carrageenan-induced rat hind paw edema, and its inhibitory effect on the chemotaxis of PMNs may also contribute to the antiinflammatory effect.
Naito
Yukiko
Y
Laboratory of Pharmacology, Hatano Research Institute, Food and Drug Safety Center, Hadano, Kanagawa, Japan. naito.yki@fdsc.or.jp
Yasumuro
Misao
M
Kondou
Keiichiro
K
Ohara
Naoki
N
D000287:Administration, Topical; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D002351:Carrageenan; D002478:Cells, Cultured; D002633:Chemotaxis / Q000187:drug effects; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy*; D006801:Humans; D008297:Male; D009504:Neutrophils / Q000187:drug effects; D011429:Propolis / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000893:Anti-Inflammatory Agents; D002351:Carrageenan; D011429:Propolis