Requirement of a mip-like gene for virulence in the phytopathogenic bacterium Xanthomonas campestris pv. campestris.
Macrophage infectivity potentiators (Mips) are FKBP domain-containing proteins reported as virulence factors in several human pathogens, such as members of genera Legionella, Salmonella and Chlamydia. The putative peptidylprolyl cis-trans isomerase (PPIase) encoded by XC2699 of the plant bacterial pathogen Xanthomonas campestris pv. campestris 8004 exhibits a 49% similarity at the amino-acid level to the Mip protein of Legionella pneumophila. This mip-like gene, XC2699, was overexpressed in Escherichia coli and the purified (His)6-tagged Mip-like protein encoded by XC2699 exhibited a PPIase activity specifically inhibited by FK-506. A mutation in the mip-like gene XC2699 led to significant reductions in virulence and replication capacity in the host plant Chinese radish (Raphanus sativus L. var. radiculus Pers.). Furthermore, the production of exopolysaccharide and the activity of extracellular proteases, virulence factors of X. campestris pv. campestris, were significantly decreased in the mip-like mutant. These results reveal that the mip-like gene is involved in the pathogenesis of X. campestris pv. campestris through an effect on the production of these virulence factors.
Zang
Ning
N
College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China.
Tang
Dong-Jie
DJ
Wei
Mei-Liang
ML
He
Yong-Qiang
YQ
Chen
Baoshan
B
Feng
Jia-Xun
JX
Xu
Jing
J
Gan
Yong-Qi
YQ
Jiang
Bo-Le
BL
Tang
Ji-Liang
JL
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D016572:Cyclosporine / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects; D008070:Lipopolysaccharides / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D010447:Peptide Hydrolases / Q000378:metabolism; D019696:Peptidylprolyl Isomerase / Q000235:genetics* / Q000378:metabolism; D019897:Periplasm / Q000201:enzymology; D010935:Plant Diseases / Q000382:microbiology; D011994:Recombinant Proteins / Q000378:metabolism; D016415:Sequence Alignment; D016559:Tacrolimus / Q000494:pharmacology; D013997:Time Factors; D014774:Virulence / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism; D016959:Xanthomonas campestris / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity
D001426:Bacterial Proteins; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D037521:Virulence Factors; D016572:Cyclosporine; D010447:Peptide Hydrolases; C519620:XC2699 protein, Xanthomonas campestris; C512922:Mip protein, Legionella pneumophila; D019696:Peptidylprolyl Isomerase; D016559:Tacrolimus