Haemoperfused liver as an ex vivo model for organ invasion of Candida albicans.
To study invasion of the human fungal pathogen Candida albicans, several infection models have been established. This study describes the successful establishment of an ex vivo haemoperfused liver as a model to study invasion of C. albicans. Perfused organs from pigs could be kept functional for up to 12 h. By comparing a non-invasive and invasive strain of C. albicans and by following a time course of invasion, it was shown that the invasion process in the perfused liver infection model is very similar to the in vivo situation after intraperitoneal infection of mice. The advantage of this set-up compared with other models of invasion is discussed.
Thewes
Sascha
S
Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany.
Reed
Hilde-Kristin
HK
Department of Comparative Medicine and Experimental Animal Science, Charité School of Medicine, 13353 Berlin, Germany.
Grosse-Siestrup
Christian
C
Department of Comparative Medicine and Experimental Animal Science, Charité School of Medicine, 13353 Berlin, Germany.
Groneberg
David A
DA
Department of Comparative Medicine and Experimental Animal Science, Charité School of Medicine, 13353 Berlin, Germany.
Meissler
Michael
M
Department of Comparative Medicine and Experimental Animal Science, Charité School of Medicine, 13353 Berlin, Germany.
Schaller
Martin
M
Institute of Dermatology, Eberhard Karls University, 72076 Tübingen, Germany.
Hube
Bernhard
B
Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany.
D000818:Animals; D002176:Candida albicans / Q000254:growth & development / Q000472:pathogenicity*; D002177:Candidiasis / Q000382:microbiology*; D004195:Disease Models, Animal; D005260:Female; D006464:Hemoperfusion; D006651:Histocytochemistry; D066298:In Vitro Techniques; D008099:Liver / Q000382:microbiology*; D008107:Liver Diseases / Q000382:microbiology*; D008297:Male; D013552:Swine; D013997:Time Factors