NopM and NopD are rhizobial nodulation outer proteins: identification using LC-MALDI and LC-ESI with a monolithic capillary column.
We have explored the potential of commercial polystyrene-divinylbenzene monolithic capillary nanoLC-MS/MS for identifying Sinorhizobium fredii HH103 nodulation outer proteins. Monolithic nanoLC with off-line MALDI-TOF/TOF and on-line ESI-q-oTOF is fast and robust, generating complementary data and offering high-confidence protein identifications from gel bands too weak for successful analysis using traditional approaches. This has allowed identification of two proteins not previously described as being type III-secreted in rhizobia, NopM and NopD.
Rodrigues
João A
JA
Department of Chemistry, University of York, Heslington, York, United Kingdom.
López-Baena
Francisco Javier
FJ
Ollero
Francisco Javier
FJ
Vinardell
José María
JM
Espuny
María Del Rosario
Mdel R
Bellogín
Ramon A
RA
Ruiz-Sainz
José Enrique
JE
Thomas
Jerry R
JR
Sumpton
Dave
D
Ault
James
J
Thomas-Oates
Jane
J
D001425:Bacterial Outer Membrane Proteins / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000302:isolation & purification*; D002853:Chromatography, Liquid; D036103:Nanotechnology / Q000295:instrumentation; D042181:Sinorhizobium fredii / Q000302:isolation & purification*; D013025:Glycine max / Q000382:microbiology; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins