title: Artificial reporter gene providing MRI contrast based on proton exchange.
abstract: Existing magnetic resonance reporter genes all rely on the presence of (super)paramagnetic substances and employ water relaxation to gain contrast. We designed a nonmetallic, biodegradable, lysine rich-protein (LRP) reporter, the prototype of a potential family of genetically engineered reporters expressing artificial proteins with frequency-selective contrast. This endogenous contrast, based on transfer of radiofrequency labeling from the reporter's amide protons to water protons, can be switched on and off.
authors.affiliation: The Russell H. Morgan Department of Radiology and Radiological Science, Neurosection, Division of MR Research, Traylor Building Rm. 217, 720 Rutland Ave., Baltimore, Maryland 21205, USA.
authors.forename: Assaf A
authors.initials: AA
authors.lastname: Gilad
authors.forename: Michael T
authors.initials: MT
authors.lastname: McMahon
authors.forename: Piotr
authors.initials: P
authors.lastname: Walczak
authors.forename: Paul T
authors.initials: PT
authors.lastname: Winnard
authors.forename: Venu
authors.initials: V
authors.lastname: Raman
authors.forename: Hanneke W M
authors.initials: HW
authors.lastname: van Laarhoven
authors.forename: Cynthia M
authors.initials: CM
authors.lastname: Skoglund
authors.forename: Jeff W M
authors.initials: JW
authors.lastname: Bulte
authors.forename: Peter C M
authors.initials: PC
authors.lastname: van Zijl
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D003287:Contrast Media; D017930:Genes, Reporter; D005910:Glioma / Q000473:pathology*; D007089:Image Enhancement / Q000379:methods*; D008239:Lysine / Q000235:genetics; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D011522:Protons; D011994:Recombinant Proteins
chemical_list: D003287:Contrast Media; D011522:Protons; D011994:Recombinant Proteins; D008239:Lysine