title: Mapping of KIT adjacent sequences on canid autosomes and B chromosomes.
abstract: B chromosomes are often considered to be one of the most mysterious elements of karyotypes (Camacho, 2004). It is generally believed that mammalian B chromosomes do not contain any protein coding genes. The discovery of a conserved KIT gene in Canidae B chromosomes has changed this view. Here we performed analysis of sequences surrounding KIT in B chromosomes of the fox and raccoon dog. The presence of the RPL23A pseudogene was shown in canid B chromosomes. The 3' end fragment of the KDR gene was found in raccoon dog B chromosomes. The size of the B-specific fragment homologous to the autosome fragment was estimated to be a minimum of 480 kbp in both species. The origin and evolution of B chromosomes in Canidae are discussed.
authors.affiliation: Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia.
authors.forename: D V
authors.initials: DV
authors.lastname: Yudkin
authors.forename: V A
authors.initials: VA
authors.lastname: Trifonov
authors.forename: A V
authors.initials: AV
authors.lastname: Kukekova
authors.forename: N V
authors.initials: NV
authors.lastname: Vorobieva
authors.forename: N V
authors.initials: NV
authors.lastname: Rubtsova
authors.forename: F
authors.initials: F
authors.lastname: Yang
authors.forename: G M
authors.initials: GM
authors.lastname: Acland
authors.forename: M A
authors.initials: MA
authors.lastname: Ferguson-Smith
authors.forename: A S
authors.initials: AS
authors.lastname: Graphodatsky
mesh_terms: D000818:Animals; D002871:Chromosome Banding; D002874:Chromosome Mapping / Q000379:methods*; D002875:Chromosomes / Q000648:ultrastructure*; D004285:Dogs; D005589:Foxes; D015723:Gene Library; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008957:Models, Genetic; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics*; D046031:Raccoon Dogs
chemical_list: D019009:Proto-Oncogene Proteins c-kit