title: Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88.
abstract: The filamentous fungus Aspergillus niger is widely exploited by the fermentation industry for the production of enzymes and organic acids, particularly citric acid. We sequenced the 33.9-megabase genome of A. niger CBS 513.88, the ancestor of currently used enzyme production strains. A high level of synteny was observed with other aspergilli sequenced. Strong function predictions were made for 6,506 of the 14,165 open reading frames identified. A detailed description of the components of the protein secretion pathway was made and striking differences in the hydrolytic enzyme spectra of aspergilli were observed. A reconstructed metabolic network comprising 1,069 unique reactions illustrates the versatile metabolism of A. niger. Noteworthy is the large number of major facilitator superfamily transporters and fungal zinc binuclear cluster transcription factors, and the presence of putative gene clusters for fumonisin and ochratoxin A synthesis.
authors.affiliation: DSM Food Specialties, PO Box 1, 2600 MA Delft, The Netherlands.
authors.forename: Herman J
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authors.lastname: Pel
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authors.lastname: de Winde
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authors.lastname: Archer
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authors.lastname: Dyer
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authors.lastname: Bendtsen
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authors.lastname: Contreras
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authors.forename: Etienne G J
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authors.lastname: Dekker
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authors.lastname: d'Enfert
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authors.forename: Coenie
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authors.lastname: Henrissat
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authors.lastname: van den Hombergh
authors.forename: Cees A M J J
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authors.lastname: van den Hondel
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authors.lastname: van der Heijden
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authors.lastname: van der Kaaij
authors.forename: Frans M
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authors.lastname: Klis
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authors.lastname: Kools
authors.forename: Christian P
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authors.lastname: Kubicek
authors.forename: Patricia A
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authors.lastname: van Kuyk
authors.forename: Jürgen
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authors.lastname: Lauber
authors.forename: Xin
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authors.lastname: Lu
authors.forename: Marc J E C
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authors.lastname: van der Maarel
authors.forename: Rogier
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authors.lastname: Meulenberg
authors.forename: Hildegard
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authors.lastname: Menke
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authors.initials: MA
authors.lastname: Mortimer
authors.forename: Jens
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authors.lastname: Nielsen
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authors.lastname: Oliver
authors.forename: Maurien
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authors.lastname: Olsthoorn
authors.forename: Karoly
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authors.lastname: Pal
authors.forename: Noël N M E
authors.initials: NN
authors.lastname: van Peij
authors.forename: Arthur F J
authors.initials: AF
authors.lastname: Ram
authors.forename: Ursula
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authors.lastname: Rinas
authors.forename: Johannes A
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authors.lastname: Roubos
authors.forename: Cees M J
authors.initials: CM
authors.lastname: Sagt
authors.forename: Monika
authors.initials: M
authors.lastname: Schmoll
authors.forename: Jibin
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authors.lastname: Sun
authors.forename: David
authors.initials: D
authors.lastname: Ussery
authors.forename: Janos
authors.initials: J
authors.lastname: Varga
authors.forename: Wouter
authors.initials: W
authors.lastname: Vervecken
authors.forename: Peter J J
authors.initials: PJ
authors.lastname: van de Vondervoort
authors.forename: Holger
authors.initials: H
authors.lastname: Wedler
authors.forename: Han A B
authors.initials: HA
authors.lastname: Wösten
authors.forename: An-Ping
authors.initials: AP
authors.lastname: Zeng
authors.forename: Albert J J
authors.initials: AJ
authors.lastname: van Ooyen
authors.forename: Jaap
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authors.forename: Hein
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authors.lastname: Stam
mesh_terms: D001234:Aspergillus niger / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal / Q000235:genetics*; D016681:Genome, Fungal / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D010940:Plant Proteins