title: Identification of steroid hormone signaling pathway in insect cell differentiation.
abstract: To dissect the steroid hormone signaling pathway involved in insect cell morphological differentiation, we extended the application of the double-stranded RNA-mediated interference (dsRNAi) method to the epidermal IAL-PID2 cell line from Plodia interpunctella Lepidoptera. We first demonstrated that dsRNA was capable of efficiently blocking the steroid hormone 20-hydroxyecdysone (20E) inducibility of proteins that belong to the nuclear receptor superfamily, including the ecdysone receptor (EcR), its partner Ultraspiracle (USP), the insect homolog of the vertebrate retinoid X receptor and the HR3 transcription factor. We then showed that inhibiting the 20E induction of EcR, USP or HR3 proteins prevented the increased synthesis of beta tubulin and consequently the morphological transformation of cells. Thanks to this functional approach, we have shown, for the first time, the participation of EcR, USP and HR3 in a 20E signaling pathway that directs morphological differentiation in insect cells by regulating beta tubulin expression.
authors.affiliation: UMR 1272 Physiologie de l'Insecte, Signalisation et Communication, Université Pierre et Marie Curie, 12 rue Cuvier, 75005 Paris, France.
authors.forename: D
authors.initials: D
authors.lastname: Siaussat
authors.forename: F
authors.initials: F
authors.lastname: Bozzolan
authors.forename: P
authors.initials: P
authors.lastname: Porcheron
authors.forename: S
authors.initials: S
authors.lastname: Debernard
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D004441:Ecdysterone / Q000378:metabolism* / Q000494:pharmacology; D000078404:Epidermal Cells; D004817:Epidermis / Q000187:drug effects; D019476:Insect Proteins / Q000378:metabolism; D007313:Insecta / Q000166:cytology* / Q000187:drug effects; D011485:Protein Binding / Q000187:drug effects; D034622:RNA Interference; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors; D014404:Tubulin / Q000378:metabolism
chemical_list: D019476:Insect Proteins; D014404:Tubulin; D004441:Ecdysterone