title: Characterization of a Suspension Culture of Tabernaemontana elegans on Growth, Nutrient Uptake, and Accumulation of Indole Alkaloids*.
abstract: A suspension culture of TABERNAEMONTANA ELEGANS was characterized on growth, nutrient uptake (carbohydrate, nitrogen, and phosphate), and on accumulation of indole alkaloids. Besides tryptamine seven indole alkaloids were isolated and identified: vobasine, vobasinol, perivine, isositsirikine, apparicine, tubotaiwine, and O-acetylvallesamine. In spite of the presence of vobasinol as major product, only trace amounts of dimeric indole alkaloids could be detected. Alkaloid formation started at the early stationary phase.
authors.affiliation: Center for Bio-Pharmaceutical Sciences, Division of Pharmacognosy, State University of Leiden, P.O. Box 9502, 2300 RA Leiden, The Netherlands.
authors.forename: R
authors.initials: R
authors.lastname: van der Heijden
authors.forename: C L
authors.initials: CL
authors.lastname: Louwe
authors.forename: E R
authors.initials: ER
authors.lastname: Verhey
authors.forename: P A
authors.initials: PA
authors.lastname: Harkes
authors.forename: R
authors.initials: R
authors.lastname: Verpoorte