[A component of earthworm fibrinolytic enzyme having higher thrombolytic activity than total components in vivo].
OBJECTIVE
To select higher thrombolytic and lower toxic single component of earthworm fibrinolytic enzymes (EFE).


METHODS
EFE containing total components were obtained by affinity chromatography from Eisenia fetida. Using ion-exchange chromatography to separate three main components EfP-0-2, EfP-I-1 and EfP-I-2 from EFE, their thrombolytic activity and toxicity were compared with EFE.


RESULTS
Among these components, EfP-I-1 had higher thrombolytic activity in vitro. When 4.5 mg x kg(-1) of these components were injected, the contents of fibrinogen in rat serum were not affected, but only EfP-I-1 exhibited distinct thrombolytic activity. When 6.0 mg x kg(-1) of them were injected intravenously, the bleeding time was not evidently delayed only by EfP-I-1. The acute toxicity test showed that the LD50 of EfP-I-1 was higher than EFE by 2. 17 times.


CONCLUSIONS
Because of distinct thrombolytic activity, lower toxicity in vivo, higher content in EFE and easy to purify, EfP-I-1 was adapted to be developed as a single component medicine for treating thrombus.
Zhao
Xiao-yu
XY
Life Science College of Hebei University, Baoding 071002, China. uyoaix@yahoo.com.cn
Liu
Ze-ming
ZM
Jing
Tian-yu
TY
Wu
Jin-xia
JX
Zhao
Zhuan-you
ZY
D000595:Amino Acid Sequence; D000818:Animals; D001760:Bleeding Time; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005340:Fibrinogen / Q000378:metabolism; D005343:Fibrinolytic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009835:Oligochaeta / Q000737:chemistry / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar; D020539:Sequence Analysis, Protein; D013055:Spectrophotometry, Infrared; D020246:Venous Thrombosis / Q000097:blood / Q000188:drug therapy*
D005343:Fibrinolytic Agents; D005340:Fibrinogen