title: Changes in salivary proteome following allogeneic hematopoietic stem cell transplantation.
abstract: OBJECTIVE
  Allogeneic hematopoietic stem cell transplantation (allo-HCT) is frequently complicated by severe infections and graft-vs-host disease (GVHD). Saliva contains many components of adaptive and innate immune response crucial for local host defenses. Changes in salivary constituents could reflect systemic processes such as immune reconstitution and development of GVHD that occur posttransplant. This study was an initial evaluation of salivary protein changes that occur after allo-HCT.
  
  
  METHODS
  Serially collected saliva samples from 41 patients undergoing allo-HCT were evaluated. Changes in salivary proteome were initially examined by SELDI-TOF mass spectrometry. Individual protein changes were identified by 2-dimensional differential in-gel electrophoresis (2D-DIGE) with subsequent MS/MS sequencing and ELISA.
  
  
  RESULTS
  Significant increases and decreases in multiple salivary proteins that lasted at least 2 months posttransplant were detected by SELDI-TOF mass spectrometry. Lactoferrin and secretory leukocyte protease inhibitor demonstrated elevations 1 month post-HCT that persisted at least 6 months. Secretory IgA (sIgA) levels were decreased 1 month posttransplant, with recovery at approximately 6 months. Levels of salivary beta(2)-microglobulin were elevated at 6 months and correlated with sIgA levels.
  
  
  CONCLUSIONS
  Allo-HCT is associated with long-term changes in several salivary proteins important for innate immune responses. These results support further studies on the association of salivary proteins with posttransplant complications including infections and GVHD.
authors.affiliation: Clinical Research Core, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Matin M
authors.initials: MM
authors.lastname: Imanguli
authors.forename: Jane C
authors.initials: JC
authors.lastname: Atkinson
authors.forename: Kristen E
authors.initials: KE
authors.lastname: Harvey
authors.forename: Gerard T
authors.initials: GT
authors.lastname: Hoehn
authors.forename: Ok Hee
authors.initials: OH
authors.lastname: Ryu
authors.forename: Tianxia
authors.initials: T
authors.lastname: Wu
authors.forename: Albert
authors.initials: A
authors.lastname: Kingman
authors.forename: A John
authors.initials: AJ
authors.lastname: Barrett
authors.forename: Michael R
authors.initials: MR
authors.lastname: Bishop
authors.forename: Richard W
authors.initials: RW
authors.lastname: Childs
authors.forename: Daniel H
authors.initials: DH
authors.lastname: Fowler
authors.forename: Steven Z
authors.initials: SZ
authors.lastname: Pavletic
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Hart
mesh_terms: D000328:Adult; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006086:Graft vs Host Disease / Q000175:diagnosis / Q000276:immunology; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007781:Lactoferrin / Q000032:analysis; D008297:Male; D015999:Multivariate Analysis; D020543:Proteome / Q000737:chemistry*; D012463:Saliva / Q000737:chemistry*; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D053481:Secretory Leukocyte Peptidase Inhibitor / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods; D014184:Transplantation, Homologous; D001613:beta 2-Microglobulin / Q000097:blood
chemical_list: D007070:Immunoglobulin A; D020543:Proteome; D012471:Salivary Proteins and Peptides; D053481:Secretory Leukocyte Peptidase Inhibitor; D001613:beta 2-Microglobulin; D007781:Lactoferrin