A novel alternative spliced variant of neutrophil gelatinase-associated lipocalin receptor in oesophageal carcinoma cells.
Recent studies suggest that NGAL (neutrophil gelatinase-associated lipocalin) is a novel iron transporter with functions distinct from that of transferrin and mediates a new iron-delivery pathway. To get a better understanding of NGAL function in oesophageal carcinoma, we analysed the expression of NGAL receptors in oesophageal carcinoma cells and identified a novel spliced variant designated NgalR-3. When expressed in a heterologous system, the protein produced from this novel spliced variant exhibits the biochemical characteristics of interaction and co-localization with NGAL protein in vivo. This new finding suggests that NgalR-3 may act as a potential NGAL receptor and play a role in NGAL-mediated iron transport in oesophageal carcinoma.
Fang
Wang-Kai
WK
Department of Biochemistry and Molecular Biology, Medical College of Shantou University, Shantou 515041, Guangdong Province, China.
Xu
Li-Yan
LY
Lu
Xiao-Feng
XF
Liao
Lian-Di
LD
Cai
Wei-Jia
WJ
Shen
Zhong-Ying
ZY
Li
En-Min
EM
D000209:Acute-Phase Proteins / Q000235:genetics* / Q000378:metabolism*; D017398:Alternative Splicing / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics; D004938:Esophageal Neoplasms / Q000201:enzymology* / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D014644:Genetic Variation / Q000235:genetics*; D006801:Humans; D000071068:Lipocalin-2; D054834:Lipocalins; D008969:Molecular Sequence Data; D027701:Organic Cation Transport Proteins; D011485:Protein Binding; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D018000:Receptors, Peptide / Q000235:genetics* / Q000378:metabolism*; D016415:Sequence Alignment
D000209:Acute-Phase Proteins; D018076:DNA, Complementary; C489506:LCN2 protein, human; D000071068:Lipocalin-2; D054834:Lipocalins; D027701:Organic Cation Transport Proteins; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D018000:Receptors, Peptide; C518841:SLC22A17 protein, human