title: UCP2 is a mitochondrial transporter with an unusual very short half-life.
abstract: This study focused on the stability of UCP2 (uncoupling protein 2), a mitochondrial carrier located in the inner membrane of mitochondrion. UCP2 is very unstable, with a half-life close to 30min, compared to 30h for its homologue UCP1, a difference that may highlight different physiological functions. Heat production by UCP1 in brown adipocytes is generally a long and adaptive phenomenon, whereas control of mitochondrial ROS by UCP2 needs more subtle regulation. We show that a mutation in UCP2 shown to modify its activity, actually decreases its stability.
authors.affiliation: CNRS-UPR 9078; Université Paris Descartes, Faculté de Médecine, site Necker, 75730 Paris Cedex 15, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Rousset
authors.forename: Julien
authors.initials: J
authors.lastname: Mozo
authors.forename: Geneviève
authors.initials: G
authors.lastname: Dujardin
authors.forename: Yalin
authors.initials: Y
authors.lastname: Emre
authors.forename: Sandrine
authors.initials: S
authors.lastname: Masscheleyn
authors.forename: Daniel
authors.initials: D
authors.lastname: Ricquier
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Cassard-Doulcier
mesh_terms: D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics; D004355:Drug Stability; D006207:Half-Life; D006801:Humans; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D000071256:Uncoupling Protein 1; D000071246:Uncoupling Protein 2
chemical_list: D007473:Ion Channels; D024101:Mitochondrial Proteins; D011994:Recombinant Proteins; C000604371:UCP1 protein, human; C000604389:UCP2 protein, human; C000604372:Ucp1 protein, mouse; C000604390:Ucp2 protein, mouse; D000071256:Uncoupling Protein 1; D000071246:Uncoupling Protein 2; D004247:DNA