Polyamines as physiological regulators of 14-3-3 interaction with the plant plasma membrane H+-ATPase.
Polyamines are abundant polycationic compounds involved in many plant physiological processes such as cell division, dormancy breaking, plant morphogenesis and response to environmental stresses. In this study, we investigated the possible role of these polycations in modulating the association of 14-3-3 proteins with the H(+)-ATPase. In vivo experiments demonstrate that, among the different polyamines, spermine brings about 2-fold stimulation of the H(+)-ATPase activity and this effect is due to an increase in 14-3-3 levels associated with the enzyme. In vivo administration of polyamine synthesis inhibitors causes a small but statistically significant decrease of the H(+)-ATPase phosphohydrolytic activity, demonstrating a physiological role for the polyamines in regulating the enzyme activity. Spermine stimulates the activity of the H(+)-ATPase AHA1 expressed in yeast, in the presence of exogenous 14-3-3 proteins, with a calculated S(50) of 70 microM. Moreover, spermine enhances the in vitro interaction of 14-3-3 proteins with the H(+)-ATPase and notably induces 14-3-3 association with the unphosphorylated C-terminal domain of the proton pump. Comparison of spermine with Mg(2+), necessary for binding of 14-3-3 proteins to different target proteins, shows that the polyamine effect is stronger than and additive to that of the divalent cation.
Garufi
Alessandra
A
Department of Biology, University of Rome Tor Vergata, via della Ricerca Scientifica, I-00133 Rome, Italy.
Visconti
Sabina
S
Camoni
Lorenzo
L
Aducci
Patrizia
P
D048948:14-3-3 Proteins / Q000737:chemistry / Q000378:metabolism*; D015317:Biogenic Polyamines / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D017494:Proton Pumps / Q000187:drug effects / Q000378:metabolism; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D013096:Spermine / Q000494:pharmacology; D003313:Zea mays / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*
D048948:14-3-3 Proteins; D015317:Biogenic Polyamines; D004268:DNA-Binding Proteins; C091848:GRF1 protein, Zea mays; D010940:Plant Proteins; D017494:Proton Pumps; D011994:Recombinant Proteins; D013096:Spermine; D006180:Proton-Translocating ATPases; D008274:Magnesium