Small-molecule regulation of zebrafish gene expression.
The zebrafish has emerged as a versatile model organism for biomedical research, yet its potential has been limited by a lack of conditional reverse-genetic tools. Here we report a chemically inducible gene expression technology that has orthogonality to vertebrate signaling processes, high induction levels, and rapid kinetics. Coupled with tissue-specific promoters, this system provides multidimensional control of gene expression and will enable new models of human disorders and diseases.
Esengil
Hanife
H
Department of Chemical and Systems Biology, Stanford University School of Medicine, 269 Campus Drive, Center for Clinical Sciences Research, Room 3155, Stanford, California 94305, USA.
Chang
Vicky
V
Mich
John K
JK
Chen
James K
JK
D000818:Animals; D002460:Cell Line; D004625:Embryo, Nonmammalian; D005786:Gene Expression Regulation / Q000502:physiology*; D023281:Genomics / Q000379:methods*; D017405:Herpes Simplex Virus Protein Vmw65 / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D007313:Insecta; D011987:Receptors, Steroid / Q000235:genetics / Q000502:physiology; D015534:Trans-Activators / Q000235:genetics; D015027:Zebrafish / Q000235:genetics*
D017405:Herpes Simplex Virus Protein Vmw65; D011987:Receptors, Steroid; D015534:Trans-Activators; C055469:ecdysone receptor