title: Prediction of the functional class of metal-binding proteins from sequence derived physicochemical properties by support vector machine approach.
abstract: Metal-binding proteins play important roles in structural stability, signaling, regulation, transport, immune response, metabolism control, and metal homeostasis. Because of their functional and sequence diversity, it is desirable to explore additional methods for predicting metal-binding proteins irrespective of sequence similarity. This work explores support vector machines (SVM) as such a method. SVM prediction systems were developed by using 53,333 metal-binding and 147,347 non-metal-binding proteins, and evaluated by an independent set of 31,448 metal-binding and 79,051 non-metal-binding proteins. The computed prediction accuracy is 86.3%, 81.6%, 83.5%, 94.0%, 81.2%, 85.4%, 77.6%, 90.4%, 90.9%, 74.9% and 78.1% for calcium-binding, cobalt-binding, copper-binding, iron-binding, magnesium-binding, manganese-binding, nickel-binding, potassium-binding, sodium-binding, zinc-binding, and all metal-binding proteins respectively. The accuracy for the non-member proteins of each class is 88.2%, 99.9%, 98.1%, 91.4%, 87.9%, 94.5%, 99.2%, 99.9%, 99.9%, 98.0%, and 88.0% respectively. Comparable accuracies were obtained by using a different SVM kernel function. Our method predicts 67% of the 87 metal-binding proteins non-homologous to any protein in the Swissprot database and 85.3% of the 333 proteins of known metal-binding domains as metal-binding. These suggest the usefulness of SVM for facilitating the prediction of metal-binding proteins. Our software can be accessed at the SVMProt server http://jing.cz3.nus.edu.sg/cgi-bin/svmprot.cgi.
authors.affiliation: Bioinformatics and Drug Design Group, Department of Pharmacy, National University of Singapore, Blk SOC1, Level 7, 3 Science Drive 2, Singapore 117543. honghuang@nus.edu.sg
authors.forename: H H
authors.initials: HH
authors.lastname: Lin
authors.forename: L Y
authors.initials: LY
authors.lastname: Han
authors.forename: H L
authors.initials: HL
authors.lastname: Zhang
authors.forename: C J
authors.initials: CJ
authors.lastname: Zheng
authors.forename: B
authors.initials: B
authors.lastname: Xie
authors.forename: Z W
authors.initials: ZW
authors.lastname: Cao
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Chen
mesh_terms: D000465:Algorithms; D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry*; D006801:Humans; D008670:Metals / Q000378:metabolism; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000145:classification* / Q000378:metabolism; D020539:Sequence Analysis, Protein / Q000379:methods*; D040681:Structural Homology, Protein
chemical_list: D002352:Carrier Proteins; D008670:Metals; D011506:Proteins