[Isolation, culture, and differentiation induction of nestin-positive cells in fetal rat hepatic cells].
OBJECTIVE
To establish the method for isolation, culture, and differentiation induction of nestin-positive cells in fetal rat hepatic cells.


METHODS
Hepatic cells were obtained from fetal rats by means of mechanical separation and hanging-drop culture, and after two days of primary culture, the medium was changed for further cell culture in the presence of 20% fetal bovine serum (containing glucose 25 ml/L, mycillin 100 U/ml, pH 7.6), 10 mmol/L nicotinamide, 1 mg/L insulin, affix N2, basic fibroblast growth factor, stem cell factor, epidermal growth factor and leukemia inhibitory factor.


CONCLUSIONS
Nestin-positive cells were obtained from fetal rat liver, which can differentiate into islet beta cells after culture and expansion in vitro.
Liu
Jun
J
Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China.
An
Jing
J
Cehn
Jin-quan
JQ
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002469:Cell Separation / Q000379:methods; D054338:Cell Transdifferentiation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D005333:Fetus; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology; D007381:Intermediate Filament Proteins / Q000096:biosynthesis*; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects; D053605:Leukemia Inhibitory Factor / Q000494:pharmacology; D008099:Liver; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D064231:Nestin; D051381:Rats; D017208:Rats, Wistar; D019089:Stem Cell Factor / Q000494:pharmacology
D007328:Insulin; D007381:Intermediate Filament Proteins; D053605:Leukemia Inhibitory Factor; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin; D019089:Stem Cell Factor; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor