Estrogen stimulates the neuronal differentiation of human umbilical cord blood mesenchymal stem cells (CD34-).
This study evaluated the effects of estrogen on the neuronal differentiation of human umbilical cord blood mesenchymal stem cells. Human umbilical cord blood mesenchymal stem cells cultured in a neuronal differentiation medium containing dimethylsulfoxide and butylated hydroxyanisole showed the expression of the neuronal cell-specific protein marker, beta-tubulin III. The estrogen treatment increased the proportion of neurons and neurite branching but reduced the mean neurite length. The relative expression of neurotropic factors such as brain-derived neurotropic factor, glial cell derived neurotropic factor, nerve growth factor, neurotrophin-3, and growth-associated protein 43 were higher in the estrogen-treated group than in the nontreated and estrogen receptor antagonist (ICI-182,780)-treated groups. These results suggest that estrogen stimulates the differentiation of neurons derived from human umbilical cord blood mesenchymal stem cells through the gene expression of neurotrophic factors.
Kang
Ji Hye
JH
School of Biotechnology and Biomedical Science, Inje University, Gimhae 621-749, Korea.
Lee
Chae Kwan
CK
Kim
Ju Ran
JR
Yu
Seong Jin
SJ
Jo
Jong Hee
JH
Do
Byung-Rok
BR
Kim
Hae Kwon
HK
Kang
Sung Goo
SG
D002454:Cell Differentiation / Q000187:drug effects*; D004347:Drug Interactions; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology; D004965:Estrogen Antagonists / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology*; D000077267:Fulvestrant; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014470:Umbilical Cord / Q000166:cytology*
D004965:Estrogen Antagonists; D004967:Estrogens; D009414:Nerve Growth Factors; D000077267:Fulvestrant; D004958:Estradiol