Artificial reporter gene providing MRI contrast based on proton exchange.
Existing magnetic resonance reporter genes all rely on the presence of (super)paramagnetic substances and employ water relaxation to gain contrast. We designed a nonmetallic, biodegradable, lysine rich-protein (LRP) reporter, the prototype of a potential family of genetically engineered reporters expressing artificial proteins with frequency-selective contrast. This endogenous contrast, based on transfer of radiofrequency labeling from the reporter's amide protons to water protons, can be switched on and off.
Gilad
Assaf A
AA
The Russell H. Morgan Department of Radiology and Radiological Science, Neurosection, Division of MR Research, Traylor Building Rm. 217, 720 Rutland Ave., Baltimore, Maryland 21205, USA.
McMahon
Michael T
MT
Walczak
Piotr
P
Winnard
Paul T
PT
Raman
Venu
V
van Laarhoven
Hanneke W M
HW
Skoglund
Cynthia M
CM
Bulte
Jeff W M
JW
van Zijl
Peter C M
PC
D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D003287:Contrast Media; D017930:Genes, Reporter; D005910:Glioma / Q000473:pathology*; D007089:Image Enhancement / Q000379:methods*; D008239:Lysine / Q000235:genetics; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D011522:Protons; D011994:Recombinant Proteins
D003287:Contrast Media; D011522:Protons; D011994:Recombinant Proteins; D008239:Lysine