Isolation, characterization and optimization of culture parameters for production of an alkaline protease isolated from Aspergillus tamarii.
Aspergillus tamarii expresses an extracellular alkaline protease that we show to be effective in removing hair from cattle hide. Large quantities of the enzyme will be required for the optimization of the enzymatic dehairing process so the growth conditions for maximum protease expression by A. tamarii were optimized for both solid-state culture on wheat bran and for broth culture. Optimal protease expression occurred, for both cultural media, at initial pH 9; the culture was incubated at 30 degrees C for 96 h using a 5% inoculum. The crude enzyme was isolated, purified and characterized using MALDI TOF TOF. The alkaline protease was homologous to the alkaline protease expressed by Aspergillus viridinutans.
Anandan
Dayanandan
D
U.S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA 19038, USA.
Marmer
William N
WN
Dudley
Robert L
RL
D001230:Aspergillus / Q000201:enzymology* / Q000254:growth & development; D001431:Bacteriological Techniques; D002244:Carbon / Q000378:metabolism; D018929:Cell Culture Techniques; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D012697:Serine Endopeptidases / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013696:Temperature
D003470:Culture Media; D005656:Fungal Proteins; D002244:Carbon; D012697:Serine Endopeptidases; C032923:microbial serine proteinases; D009584:Nitrogen