title: Resistin-binding peptide antagonizes role of resistin on white adipose tissue.
abstract: OBJECTIVE
  To investigate the direct effects of resistin and resistin-binding peptide (RBP) on lipid metabolism and endocrine function in adipose tissue.
  
  
  METHODS
  Rat white adipose tissue was cultured in vitro and incubated for 24 h with 30 ng/mL recombinant rat resistin protein (rResistin) or combined with RBP of varying concentrations(1x10(-12) mol/L, 1x10(-10) mol/L, 1x10(-8) mol/L). Free fatty acids (FFA) released into medium was measured by a colorimetric kit. The levels of protein secretion and mRNA expression of TNF-alpha and adiponectin were detected by ELISA kit and RT-PCR respectively.
  
  
  RESULTS
  The levels of FFA released into medium were significantly increased after 24 h of exposure to rResistin, but significantly decreased after RBP was applied, although there was no difference between the 3 concentrations. The protein level and gene expression of TNF-alpha in adipose tissue were significantly increased after 24 h of exposure to rResistin, but only obviously decreased after incubated with 1x10(-8) mol/L RBP. The levels of protein secretion and mRNA expression of adiponectin in adipose tissue were significantly decreased after 24 h of exposure to rResistin, but increased after incubated with RBP with the higher concentrations.
  
  
  CONCLUSIONS
  RBP can effectively antagonize the role of resistin on the lipid metabolism and endocrine function of adipose tissue.
authors.affiliation: Department of Pediatrics, Nanjing Maternal and Child Health Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Gu
authors.forename: Shu-ping
authors.initials: SP
authors.lastname: Han
authors.forename: Li
authors.initials: L
authors.lastname: Fei
authors.forename: Xiao-qin
authors.initials: XQ
authors.lastname: Pan
authors.forename: Mei
authors.initials: M
authors.lastname: Guo
authors.forename: Rong-hua
authors.initials: RH
authors.lastname: Chen
authors.forename: Xi-rong
authors.initials: XR
authors.lastname: Guo
mesh_terms: D052242:Adiponectin / Q000235:genetics; D052436:Adipose Tissue, White / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins / Q000502:physiology*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D008066:Lipolysis; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D052243:Resistin / Q000037:antagonists & inhibitors / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D052242:Adiponectin; D002352:Carrier Proteins; D005230:Fatty Acids, Nonesterified; D052243:Resistin; C499622:Retn protein, rat; D014409:Tumor Necrosis Factor-alpha