title: NopM and NopD are rhizobial nodulation outer proteins: identification using LC-MALDI and LC-ESI with a monolithic capillary column.
abstract: We have explored the potential of commercial polystyrene-divinylbenzene monolithic capillary nanoLC-MS/MS for identifying Sinorhizobium fredii HH103 nodulation outer proteins. Monolithic nanoLC with off-line MALDI-TOF/TOF and on-line ESI-q-oTOF is fast and robust, generating complementary data and offering high-confidence protein identifications from gel bands too weak for successful analysis using traditional approaches. This has allowed identification of two proteins not previously described as being type III-secreted in rhizobia, NopM and NopD.
authors.affiliation: Department of Chemistry, University of York, Heslington, York, United Kingdom.
authors.forename: João A
authors.initials: JA
authors.lastname: Rodrigues
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: López-Baena
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: Ollero
authors.forename: José María
authors.initials: JM
authors.lastname: Vinardell
authors.forename: María Del Rosario
authors.initials: Mdel R
authors.lastname: Espuny
authors.forename: Ramon A
authors.initials: RA
authors.lastname: Bellogín
authors.forename: José Enrique
authors.initials: JE
authors.lastname: Ruiz-Sainz
authors.forename: Jerry R
authors.initials: JR
authors.lastname: Thomas
authors.forename: Dave
authors.initials: D
authors.lastname: Sumpton
authors.forename: James
authors.initials: J
authors.lastname: Ault
authors.forename: Jane
authors.initials: J
authors.lastname: Thomas-Oates
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000302:isolation & purification*; D002853:Chromatography, Liquid; D036103:Nanotechnology / Q000295:instrumentation; D042181:Sinorhizobium fredii / Q000302:isolation & purification*; D013025:Glycine max / Q000382:microbiology; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins