title: Fatty acid synthase effects on bovine adipose fat and milk fat.
abstract: A quantitative trait locus (QTL) was identified by linkage analysis on bovine Chromosome 19 that affects the fatty acid, myristic acid (C14:0), in subcutaneous adipose tissue of pasture-fed beef cattle (99% level: experiment-wise significance). The QTL was also shown to have significant effects on ten fatty acids in the milk fat of pasture-fed dairy cattle. A positional candidate gene for this QTL was identified as fatty acid synthase (FASN), which is a multifunctional enzyme with a central role in the metabolism of lipids. Five single nucleotide polymorphisms (SNPs) were identified in the bovine FASN gene, and animals were genotyped for FASN SNPs in three different cattle resource populations. Linkage and association mapping results using these SNPs were consistent with FASN being the gene underlying the QTL. SNP substitution effects for C14:0 percentage were found to have an effect in the opposite direction in adipose fat to that in milk fat. It is concluded that SNPs in the bovine FASN gene are associated with variation in the fatty acid composition of adipose fat and milk fat.
authors.affiliation: AgResearch, Ruakura Research Centre, PB 3123, Hamilton, New Zealand. chris.morris@agresearch.co.nz
authors.forename: Chris A
authors.initials: CA
authors.lastname: Morris
authors.forename: Neil G
authors.initials: NG
authors.lastname: Cullen
authors.forename: Belinda C
authors.initials: BC
authors.lastname: Glass
authors.forename: Dianne L
authors.initials: DL
authors.lastname: Hyndman
authors.forename: Tim R
authors.initials: TR
authors.lastname: Manley
authors.forename: Sharon M
authors.initials: SM
authors.lastname: Hickey
authors.forename: John C
authors.initials: JC
authors.lastname: McEwan
authors.forename: Wayne S
authors.initials: WS
authors.lastname: Pitchford
authors.forename: Cynthia D K
authors.initials: CD
authors.lastname: Bottema
authors.forename: Michael A H
authors.initials: MA
authors.lastname: Lee
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics* / Q000378:metabolism*; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D017931:DNA Primers / Q000235:genetics; D064429:Fatty Acid Synthases / Q000235:genetics*; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D006239:Haplotypes; D015810:Linkage Disequilibrium; D008297:Male; D008892:Milk / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci
chemical_list: D017931:DNA Primers; D005227:Fatty Acids; D064429:Fatty Acid Synthases