title: Occurrence and genetic variability of Candida parapsilosis sensu lato in Hungary.
abstract: The occurrence and genetic variability of Candida parapsilosis isolates in two Hungarian hospitals, located in Debrecen and Pécs, were examined. Among the 209 Candida isolates examined, 20 were found to belong to C. parapsilosis sensu lato, based on morphological, physiological and molecular data. The frequency of occurrence of C. parapsilosis isolates (9.6%) was lower than that observed in Europe but higher than that observed previously in Hungary. The genetic variability of C. parapsilosis sensu lato isolates was also examined using random amplified polymorphic DNA (RAPD) analysis and sequence analysis of the intergenic transcribed spacer (ITS) region of the rRNA gene cluster. The genetic variability of the isolates was relatively high, as revealed by RAPD analysis. Two isolates were found to belong to the recently described Candida metapsilosis species (C. parapsilosis group III), based on ITS sequence data, RAPD analysis and phenotypic data. These two isolates could also be distinguished from C. parapsilosis sensu stricto isolates using a primer pair developed for the detection of C. parapsilosis group I isolates. To the best of the authors' knowledge, this is the first report on the identification of C. metapsilosis from bloodstream infection.
authors.affiliation: Department of Microbiology, Faculty of Sciences, University of Szeged, PO Box 533, H-6701 Szeged, Hungary.
authors.forename: Sándor
authors.initials: S
authors.lastname: Kocsubé
authors.affiliation: Department of Microbiology, Faculty of Sciences, University of Szeged, PO Box 533, H-6701 Szeged, Hungary.
authors.forename: Mónika
authors.initials: M
authors.lastname: Tóth
authors.affiliation: Department of Microbiology, Faculty of Sciences, University of Szeged, PO Box 533, H-6701 Szeged, Hungary.
authors.forename: Csaba
authors.initials: C
authors.lastname: Vágvölgyi
authors.affiliation: Department of Clinical Microbiology, Faculty of Medicine, University of Szeged, PO Box 427, H-6701 Szeged, Hungary.
authors.forename: Ilona
authors.initials: I
authors.lastname: Dóczi
authors.affiliation: Department of General and Environmental Microbiology, University of Pécs, H-7601 Pécs, Hungary.
authors.forename: Miklós
authors.initials: M
authors.lastname: Pesti
authors.affiliation: Department of Microbiology and Biotechnology, Medical and Health Science Center, University of Debrecen, PO Box 63, H-4010, Debrecen, Hungary.
authors.forename: István
authors.initials: I
authors.lastname: Pócsi
authors.affiliation: Institute of Medical Microbiology, Faculty of Sciences, University of Debrecen, PO Box 63, H-4010, Debrecen, Hungary.
authors.forename: Judit
authors.initials: J
authors.lastname: Szabó
authors.affiliation: CBS Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.|Department of Microbiology, Faculty of Sciences, University of Szeged, PO Box 533, H-6701 Szeged, Hungary.
authors.forename: János
authors.initials: J
authors.lastname: Varga
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology; D002175:Candida / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D002177:Candidiasis / Q000382:microbiology*; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D016469:Fungemia / Q000382:microbiology; D006761:Hospitals; D006814:Hungary; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA
chemical_list: D000935:Antifungal Agents; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer