Synthesis of a coumarin-based europium complex for bioanalyte labeling.
A coumarin-based europium chelate ready-to-use for analyte labeling and homogeneous time-resolved fluorescence measurements has been designed. Compound 1 displays three functional elements: an azide reactive spacer arm, a coumarin sensitizer, and a seven-coordinate europium complex. That complex can be excited at 370 nm by inexpensive UV-LEDs as a light excitation source.
Féau
Clémentine
C
Institut Gilbert-Laustriat, CNRS-Université Louis Pasteur, Faculté de Pharmacie, 74 route du Rhin, 67401 Illkirch Cedex, France.
Klein
Emmanuel
E
Kerth
Paul
P
Lebeau
Luc
L
D001386:Azides / Q000737:chemistry; D002614:Chelating Agents / Q000737:chemistry*; D003374:Coumarins / Q000138:chemical synthesis* / Q000528:radiation effects; D005063:Europium / Q000737:chemistry* / Q000528:radiation effects; D007202:Indicators and Reagents; D013050:Spectrometry, Fluorescence; D014466:Ultraviolet Rays
D001386:Azides; D002614:Chelating Agents; D003374:Coumarins; D007202:Indicators and Reagents; D005063:Europium; C030123:coumarin