Evolution of mammalian CD1: marsupial CD1 is not orthologous to the eutherian isoforms and is a pseudogene in the opossum Monodelphis domestica.
CD1 is a member of the major histocompatibility complex (MHC) class I family of proteins that present lipid antigens to T cells and natural killer (NK) T cells; it is found in both eutherian mammals and birds. In eutherians, duplication of the CD1 gene has resulted in multiple isoforms. A marsupial CD1 homologue was identified in a set of expressed sequence tags from the thymus of the bandicoot Isoodon macrourus. Southern blot and genomic sequence analyses revealed that CD1 is a single copy gene in both I. macrourus and a distantly related marsupial, the opossum Monodelphis domestica, which is currently the only marsupial species for which a whole genome sequence is available. We found that the opossum CD1 is located in a genomic region with a high degree of conserved synteny to the chromosomal regions containing human and mouse CD1. A phylogenetic analysis of mammalian CD1 revealed that marsupial CD1 is not orthologous to the eutherian CD1 isoforms, consistent with the latter having emerged by duplication after the separation of marsupials and eutherians 170-180 million years ago. The I. macrourus CD1 gene is actively transcribed and appears to encode a functional protein. In contrast, transcription of the M. domestica CD1 was not detected in any tissue and the predicted CD1 gene sequence contains a number of deletions that appear to render the locus a pseudogene.
Baker
Michelle L
ML
Center for Evolutionary and Theoretical Immunology, Department of Biology, University of New Mexico, Albuquerque NM 87131, USA. mlbaker@unm.edu
Miller
Robert D
RD
D000595:Amino Acid Sequence; D000818:Animals; D018949:Antigens, CD1 / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D019143:Evolution, Molecular; D006801:Humans; D008394:Marsupialia / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D048309:Monodelphis / Q000235:genetics / Q000276:immunology; D010802:Phylogeny; D011544:Pseudogenes; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D016415:Sequence Alignment; D013045:Species Specificity; D013154:Spleen / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology
D018949:Antigens, CD1