[Immunosuppressive effect of Tibetan medicine, Artemisia vestita Wall Extract].
OBJECTIVE
To assess the immunosuppressive effect of Artemisia vestita Wall Extract (AV-ext) on mice.


METHODS
The proliferative reaction of lymphocyte and the mixed lymphocytes reaction were used to determine the effects of AV-ext on the proliferation of mouse splenocyte in vitro and in vivo; Proliferative reaction of mouse splenocyte was used for detecting the effects of AV-ext on the level of IL-2 secreted by mouse activated splenocyte in vitro. Gelatin enzymogram method and adherence analytical method were employed to disclose the effects of AV-ext on mouse activated T-lymphocytes mobility and adherence.


RESULTS
1-100 microg/mL AV-ext exerted no obvious toxicity to mouse splenocyte, but it had obvious inhibitory effect on proliferative reaction of mouse splenocyte and mixed lymphocytes reaction induced by ConA. It also had obvious inhibitory effect on the level of IL-2 secreted by mouse activated splenocyte, on the production of MMP-9 by mouse activated T-lymphocytes, and on adherence. 150 mg/kg and 300 mg/kg of AV-ext, given to mouse per os for 7 days, could inhibit the proliferation of splenocyte and the secretion of MMP-9 by activated splenocyte of mouse.


CONCLUSIONS
AV-ext can inhibit the cellular immune reaction of mouse obviously.
Wang
Ju-Le
JL
Medical College of Tibet University, Lasa 850002, China.
Li
Chang-Shan
CS
Dun
Zhu
Z
Zhou
Hui-Ying
HY
D000284:Administration, Oral; D000818:Animals; D019306:Artemisia / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects / Q000276:immunology; D049109:Cell Proliferation / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007166:Immunosuppressive Agents / Q000276:immunology* / Q000494:pharmacology; D007376:Interleukin-2 / Q000378:metabolism; D019169:Jurkat Cells; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D010853:Picryl Chloride / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D018609:Tibet
D004365:Drugs, Chinese Herbal; D007166:Immunosuppressive Agents; D007376:Interleukin-2; D003208:Concanavalin A; D020780:Matrix Metalloproteinase 9; D010853:Picryl Chloride