title: [The p16INK4a protein: a cytological marker for detecting high grade intraepithelial neoplasia of the uterine cervix].
abstract: The identification of a small percentage of high grade cervical intraepithelial neoplasia (HGCIN) among patients with a diagnosis of atypical squamous cells of undetermined significance (ASC-US) and low grade squamous intraepithelial lesion (LSIL) is one of the difficulties in cytology based cervical cancer screening. p16INK4a is a surrogate marker for the initiation of HPV mediated cervical carcinogenesis. This article describes the detection of the protein p16INK4a by immunocytochemistry coupled with the use of a nuclear score to differentiate abnormal basal cells from metaplastic or atrophic cells. The results of a pilot series of 210 liquid based cytology (LBC) specimens of which 108 were considered normal, 52 with a diagnosis of LSIL and 50 with a diagnosis of high grade SIL are described. The second series includes 137 LBC specimens with an ASC-US diagnosis and 88 with a LSIL diagnosis with an histological correlation. The overall sensitivity for the diagnosis of HGCIN using for the labeled squamous cells a nuclear score superior to 2 was 96% and the specificity was 83%. The sensitivity in the ASC-US was 95% and the specificity was 84%, in the LSIL group 100% and 81%, respectively. These data suggest to study a large series of LBC smears with a diagnosis of ASC-US and LSIL to confirm its efficacy of predicting the presence of an HGCIN.
authors.affiliation: Laboratoire Pasteur-Cerba, 95066 Cergy Pontoise Cedex 9. bergeron@pasteur-cerba.com
authors.forename: Christine
authors.initials: C
authors.lastname: Bergeron
authors.forename: Nicolas
authors.initials: N
authors.lastname: Wentzensen
authors.forename: Frederic
authors.initials: F
authors.lastname: Cas
authors.forename: Magnus
authors.initials: M
authors.lastname: von Knebel Doeberitz
mesh_terms: D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000032:analysis*; D005260:Female; D006801:Humans; D002583:Uterine Cervical Neoplasms / Q000737:chemistry* / Q000473:pathology*; D002578:Uterine Cervical Dysplasia / Q000737:chemistry* / Q000473:pathology*
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16