[Role of endothelin-1 and nitric oxide level in pathogenesis preeclampsia].
The changes in blood vessel endothelium have impact on pathogenesis of preeclampsia. The aim of the study was establishment of nitric oxide level (NO) and endothelin-1 content in blood during preeclampsia. The level of NO and content of endothelin-1 were studied in blood of healthy reproductive age nonpregnant, practically healthy pregnant patients, and patients with complicated pregnancy (preeclampsia) (III trem). The free NO content was established by electron paramagnetic resonance (EPR) method using spin-trap Na diethil-ditiocarbomate (DETC) (Sigma). Endotheline-1 content was determined by immuno-ensyzatic method with Reagent Kit (Endothelin-1, Cayman Chemical). Free NO level and endothelin-1 content in blood was similar in both--healthy reproductive age nonpregnant women and practically healthy pregnant patients. It was unchanged during physiological pregnancy. During preeclampsia the free NO content was 10% decreased, and endotheline-1 content 71% increased compared to control (physiological pregnancy) level.


CONCLUSIONS
during preeclampsia the low activity of endothelial NO-syntheses and redox-dependent transformation of NO in peroxynitrite provoke decrease nitric oxide level in blood. Increase of Endothelin-1 content in blood may be compensatory to low placental perfusion during preeclampsia. High endothelin-1 is cytotoxity, causes intensification of oxidative stress in trophoblasts and disorders of cell membranes of blood vessel endothelium. Free nitric oxide reduction and rising of endothelin-1 level in blood during preeclampsia causes redoubling of endothelial function.
Khetsuriani
T
T
Tbilisi State Medical University.
Chabashvili
N
N
Sanikidze
T
T
D019332:Endothelin-1 / Q000502:physiology*; D005260:Female; D006801:Humans; D006973:Hypertension / Q000453:epidemiology; D009569:Nitric Oxide / Q000502:physiology*; D011225:Pre-Eclampsia / Q000453:epidemiology / Q000503:physiopathology*; D011247:Pregnancy; D011248:Pregnancy Complications / Q000453:epidemiology; D011507:Proteinuria / Q000453:epidemiology
D019332:Endothelin-1; D009569:Nitric Oxide