Colony-forming cell assay for human hematopoietic progenitor cells harvested by a novel continuous-flow cell separation method.
In order to prove the functional potentiality of cells separated according to their densities by a novel continuous-flow cell separation method, the colony-forming cell (CFC) assay was performed on the harvested cells from peripheral blood and umbilical cord blood. The number of colony-forming unit-granulocyte, erythroid, macrophage, megakaryocyte (CFU-GEMM), which are considered to be stem cells, was approximately 3% of colonies developed from the peripheral blood and approximately 4% of colonies from the umbilical cord blood. It appears that this new method could allow us for harvesting of hematopoietic progenitor cells without losing their native ability to proliferate.
Shiono
Hiroyuki
H
Department of Physiology, Aichi Medical University School of Medicine, Nagakute, Aichi 480-1195, Japan. shiono-h@aichi-med-u.ac.jp
Chen
Hong Miao
HM
Okada
Tadashi
T
Ito
Yoichiro
Y
D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D003114:Colony-Forming Units Assay / Q000379:methods*; D005312:Fetal Blood / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans