title: Protection of transgenic tobacco plants expressing bovine pancreatic ribonuclease against tobacco mosaic virus.
abstract: Transgenic tobacco plants (Nicotiana tabacum cv. SR1) expressing extracellular pancreatic ribonuclease from Bos taurus and characterized by an increased level of ribonuclease activity in leaf extracts were challenged with tobacco mosaic virus. The transgenic plants exhibited a significantly higher level of protection against the virus infection than the control non-transformed plants. The protection was evidenced by the absence (or significant delay) of the appearance of typical mosaic symptoms and the retarded accumulation of infectious virus and viral antigen. These results demonstrate that modulation of extracellular nuclease expression can be efficiently used in promoting protection against viral diseases.
authors.affiliation: Institute of Cytology and Genetics, 10, Lavrentieva str, Novosibirsk 630090, Russia.
authors.forename: Ekaterina A
authors.initials: EA
authors.lastname: Trifonova
authors.forename: Mikhail V
authors.initials: MV
authors.lastname: Sapotsky
authors.forename: Marina L
authors.initials: ML
authors.lastname: Komarova
authors.forename: Andrey B
authors.initials: AB
authors.lastname: Scherban
authors.forename: Vladimir K
authors.initials: VK
authors.lastname: Shumny
authors.forename: Albina M
authors.initials: AM
authors.lastname: Polyakova
authors.forename: Larisa A
authors.initials: LA
authors.lastname: Lapshina
authors.forename: Alex V
authors.initials: AV
authors.lastname: Kochetov
authors.forename: Vladimir I
authors.initials: VI
authors.lastname: Malinovsky
mesh_terms: D000818:Animals; D002417:Cattle; D010935:Plant Diseases / Q000235:genetics* / Q000821:virology*; D030821:Plants, Genetically Modified; D012259:Ribonuclease, Pancreatic / Q000235:genetics* / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism / Q000821:virology*; D014027:Tobacco Mosaic Virus / Q000502:physiology*
chemical_list: D012259:Ribonuclease, Pancreatic; C504335:pancreatic ribonuclease B, bovine