Effect of nitric oxide on phagocytic activity of lipopolysaccharide-induced macrophages: possible role of exogenous L-arginine.
Among the antimicrobial mechanisms associated with macrophages, NO produced by iNOS plays a major role in intracellular killing, but the relationship between NO and phagocytic activity after injection of inflammatory agents into the peritoneal cavity is not clear. The aim of the present study was to investigate the effect of nitric oxide (NO) on macrophage function after treatment with intraperitoneal lipopolysaccharide (LPS) and the role of exogenous L-arginine administration in this event. Six experimental groups and one control group, each consisting of seven Wistar rats were used: Group I: Control; Group II: LPS; Group III: LPS+L-arginine; Group IV: LPS+L-arginine+Aminoguanidine; Group V: LPS+Aminoguanidine; Group VI: L-arginine; Group VII: Aminoguanidine. Macrophage phagocytic activity and total plasma nitrite levels were increased in the LPS group. In the LPS+L-arginine group, both the phagocytic activity and total plasma nitrite levels showed large increases. Administration of aminoguanidine (AG), a specific iNOS inhibitor, abolished macrophage phagocytic activity and total plasma nitrite levels in the LPS and LPS+L-arginine groups. As a result, we showed that NO produced by macrophages has a role not only in intracellular killing, but also in phagocytic activity.
Tümer
Cemil
C
Department of Physiology, Faculty of Medicine, Mustafa Kemal University, Uur Mumcu Street, 31100 Hatay, Turkey. ctumer@gmail.com
Bilgin
Hakki Murat
HM
Obay
Basra Deniz
BD
Diken
Hüda
H
Atmaca
Mukadder
M
Kelle
Mustafa
M
D000818:Animals; D001120:Arginine / Q000494:pharmacology*; D006146:Guanidines / Q000008:administration & dosage / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000187:drug effects*; D008297:Male; D009569:Nitric Oxide / Q000494:pharmacology*; D009573:Nitrites / Q000097:blood; D010587:Phagocytosis / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
D006146:Guanidines; D008070:Lipopolysaccharides; D009573:Nitrites; D009569:Nitric Oxide; D001120:Arginine; C004479:pimagedine