Differential gene expression profile in spleen of mandarin fish Siniperca chuatsi infected with ISKNV, derived from suppression subtractive hybridization.
To study the interaction between an invading virus and its host, we investigated differential gene expression in mandarin fish Siniperca chuatsi experimentally infected with infectious spleen and kidney necrosis virus (ISKNV). Subtractive cDNA libraries were constructed by suppression subtractive hybridization (SSH) from spleens of mock- and ISKNV-infected fish. Both forward- and reverse-subtracted libraries were generated. In the forward library, genes of the ubiquitin-proteasome proteolytic pathway, defense-related genes, a cytoskeletal protein gene, an apoptosis-related gene encoding inhibitor of apoptosis protein and JFC/EBPb cDNA for CAAT/Enhancer binding protein beta were up-regulated after infection. In the reverse library, genes that encoded CD59/neurotoxin/Ly-6-like protein, carboxypeptidase A2, and goose-type lysozyme were down-regulated. Some of these genes were analyzed by reverse transcription-polymerase chain reaction to confirm their differential expression as a result of virus infection. The results of this study may contribute to our understanding of fish innate immune response to ISKNV.
He
Wei
W
State Key Laboratory for Biocontrol, School of Life Sciences, Zhongshan University, 135 Xingang Road West, Guangzhou 510275, PR China.
Yinlt
Zhi-Xin
ZX
Li
You
Y
Huo
Wei-Li
WL
Guan
Hao-Ji
HJ
Weng
Shao-Ping
SP
Chan
Siu-Ming
SM
He
Jian-Guo
JG
D000818:Animals; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000235:genetics / Q000378:metabolism; D016384:Consensus Sequence; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D004266:DNA Virus Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D005393:Fish Diseases / Q000276:immunology* / Q000821:virology; D015870:Gene Expression / Q000276:immunology*; D020869:Gene Expression Profiling; D015723:Gene Library; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics / Q000378:metabolism; D007497:Iridoviridae / Q000276:immunology* / Q000472:pathogenicity; D009693:Nucleic Acid Hybridization; D010473:Perciformes / Q000235:genetics* / Q000821:virology; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013154:Spleen / Q000276:immunology* / Q000503:physiopathology; D016608:Talin / Q000235:genetics / Q000378:metabolism; D025801:Ubiquitin / Q000235:genetics / Q000378:metabolism
D022782:CCAAT-Enhancer-Binding Protein-beta; D016207:Cytokines; D017931:DNA Primers; D051034:Inhibitor of Apoptosis Proteins; D012333:RNA, Messenger; D012269:Ribosomal Proteins; D016608:Talin; D025801:Ubiquitin