Osteochondral defect repair using a novel tissue engineering approach: sheep model study.
Porous calcium polyphosphate (CPP) constructs of desired density were formed by sintering CPP powders. Articular cartilage was formed on these constructs in cell culture over an 8-week period with the resulting cartilage layer forming on the CPP surface and within the near surface pores thereby mechanically anchoring the cartilage to the CPP. The biphasic constructs so formed were implanted in sheep femoral condyle sites and left for short-term periods (3 to 4 months) or longer periods (9 months). Implant fixation within the condyle sites was achieved through bone ingrowth into the inferior CPP pores. The properties and characteristics of the as-in vitro-formed, short- and long-term implanted tissues were compared. The results indicated that such implants might be useful for repair of small subchondral defects.
Pilliar
R M
RM
Faculty of Dentistry, University of Toronto, Institute of Biomaterials & Biomedical Engineering, Toronto, Canada. bob.pilliar@utoronto.ca
Kandel
R A
RA
Grynpas
M D
MD
Zalzal
P
P
Hurtig
M
M
D000818:Animals; D001672:Biocompatible Materials; D001861:Bone Regeneration; D018786:Bone Substitutes; D002130:Calcium Phosphates; D002357:Cartilage Diseases / Q000628:therapy*; D002358:Cartilage, Articular / Q000293:injuries*; D018929:Cell Culture Techniques; D019902:Chondrocytes / Q000737:chemistry*; D016348:Osseointegration; D011122:Polyphosphates; D034561:Sheep, Domestic; D023822:Tissue Engineering
D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; D011122:Polyphosphates