A sperm cytoskeletal protein TSA70 is a novel phosphorylated member of cenexin/odf2 family.
Using post-vasectomy monoclonal antibody we recently identified a testis specific sperm auto-antigen called TSA70 which is post-meiotically expressed and plays a role in sperm motility and capacitation-acrosome reaction. In the present study, we report its cytoskeletal nature based on its resistance to various high ionic salt solutions. TSA70 is developmentally regulated and appears postpubertally. The two protein spots identified by 2D WB namely TSA1-pI=5.821, MW=77.050 and TSA3-pI=6.173, MW=75.519 showed sequence homology to Cenexin/odf2 indicating that two are isoforms of the same protein. The immunoreactivity of TSA70 with anti-Cenexin antibody substantiates its homology with Cenexin/odf2. In silico analysis revealed the presence of two leucine zippers in TSA70 and also predicted potential phosphorylation sites at serine, threonine, and tyrosine residues. The phosphorylated status of TSA70 was further confirmed by immunoblot analysis. The differential cellular expression suggests that TSA70 is a novel member of Cenexin/odf2 family that exhibits functional divergence.
Wakle
Monali
M
Gamete Immunobiology Department, National Institute for Research in Reproductive Health, J.M. Street, Parel, Mumbai, India.
Khole
Vrinda
V
D000818:Animals; D001324:Autoantigens / Q000737:chemistry / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D010766:Phosphorylation; D051381:Rats; D013094:Spermatozoa / Q000737:chemistry / Q000378:metabolism*
D001324:Autoantigens; D003598:Cytoskeletal Proteins; D006360:Heat-Shock Proteins; C490105:Odf2 protein, rat; C518683:testis-specific antigen 70, rat