Hemoglobin protein hollow shells fabricated through covalent layer-by-layer technique.
Hemoglobin (Hb) protein microcapsules held together by cross-linker, glutaraldehyde (GA), were successfully fabricated by covalent layer-by-layer (LbL) technique. The Schiff base reaction occurred on the colloid templates between the aldehyde groups of GA and free amino sites of Hb results in the formation of GA/Hb microcapsules after the removal of the templates. The structure of obtained monodisperse protein microcapsule was characterized by transmission electron microscopy (TEM) and confocal laser scanning microscopy (CLSM). The UV-Vis spectra measurements demonstrate the existence of Hb in the assembled capsules. Cyclic voltammetry (CV) and potential-controlled amperometric measurements (I-t curve) confirm that hemoglobin microcapsules after fabrication remain their heme electroactivity. Moreover, direct electron transfer process from protein to electrode surface was performed to detect the heme electrochemistry without using any mediator or promoter. The experiments of fluorescence recovery after photobleaching (FRAP) by CLSM demonstrate that the hemoglobin protein microcapsules have an improved permeability comparing to the conventional polyelectrolyte microcapsules.
Duan
Li
L
Beijing National Laboratory for Molecular Sciences (BNLMS), International Joint Lab, CAS Key Lab of Colloid and Interface Science, Institute of Chemistry, Chinese Academy of Sciences, Zhong Guan Cun, Bei Yi Jie No 2, Beijing 100080, China.
He
Qiang
Q
Yan
Xuehai
X
Cui
Yue
Y
Wang
Kewei
K
Li
Junbai
J
D000818:Animals; D002214:Capsules / Q000138:chemical synthesis; D002254:Carbonates / Q000138:chemical synthesis; D002417:Cattle; D003432:Cross-Linking Reagents / Q000737:chemistry; D005976:Glutaral / Q000737:chemistry; D006454:Hemoglobins / Q000138:chemical synthesis* / Q000648:ultrastructure; D008345:Manganese; D046529:Microscopy, Electron, Transmission
D002214:Capsules; D002254:Carbonates; D003432:Cross-Linking Reagents; D006454:Hemoglobins; D008345:Manganese; C045327:manganese carbonate; D005976:Glutaral