title: The utility of peripheral thyrotropin mRNA in the diagnosis of follicular neoplasms and surveillance of thyroid cancers.
abstract: BACKGROUND
  Thyroid cells in peripheral circulation express uniquely thyrotropin receptor (TSHR) mRNA, and their detection may aid thyroid cancer management.
  
  
  METHODS
  Since 2002, 258 patients had prospective TSHR mRNA measurement by quantitative RT-PCR from peripheral blood before and/or after thyroidectomy. Thyroid cancer detection was assessed from known clinical diagnostic criteria and mRNA for patients with follicular neoplasms (n = 64) and long-term cancer follow-up (n = 13).
  
  
  RESULTS
  Adding TSHR mRNA to fine-needle aspiration biopsy (FNAB) maintained high sensitivity (90%) but improved specificity (73%) for thyroid cancer diagnosis. When FNAB specimens indicated follicular neoplasm, a decision algorithm combining TSHR mRNA and abnormal thyroid ultrasound features correctly diagnosed all cancer patients (100% sensitivity) and would have spared operation for benign disease in 38%. Elevated TSHR mRNA on postoperative day 1 predicted persistent/recurrent cancer. During long-term thyroid cancer surveillance, TSHR mRNA had a 91% concordance with radioactive iodine whole body scan (WBS)-detectable disease, agreed with thyroglobulin (Tg) levels in 64% of patents, missed disease in 5%, but was more sensitive to detecting disease than Tg levels in 31% of patients, including all patients with Tg antibodies.
  
  
  CONCLUSIONS
  Detecting circulating thyroid cancer cells is useful for initial thyroid cancer diagnosis and postoperatively predicts recurrent cancer. This novel test promises to enhance thyroid cancer patient care by management algorithms that combine histologic, genomic, and clinical criteria.
authors.affiliation: Department of General Surgery, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA. milasm@ccf.org
authors.forename: Mira
authors.initials: M
authors.lastname: Milas
authors.forename: Peter
authors.initials: P
authors.lastname: Mazzaglia
authors.forename: Su-Ynn
authors.initials: SY
authors.lastname: Chia
authors.forename: Mario
authors.initials: M
authors.lastname: Skugor
authors.forename: Eren
authors.initials: E
authors.lastname: Berber
authors.forename: Sethu
authors.initials: S
authors.lastname: Reddy
authors.forename: Manjula
authors.initials: M
authors.lastname: Gupta
authors.forename: Allan
authors.initials: A
authors.lastname: Siperstein
mesh_terms: D000236:Adenoma / Q000175:diagnosis; D000328:Adult; D000368:Aged; D000465:Algorithms; D044963:Biopsy, Fine-Needle; D002291:Carcinoma, Papillary / Q000175:diagnosis* / Q000000981:diagnostic imaging / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009360:Neoplastic Cells, Circulating / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D011989:Receptors, Thyrotropin / Q000378:metabolism*; D013964:Thyroid Neoplasms / Q000175:diagnosis* / Q000000981:diagnostic imaging / Q000378:metabolism; D014463:Ultrasonography
chemical_list: D012333:RNA, Messenger; D011989:Receptors, Thyrotropin