title: Estrogen stimulates the neuronal differentiation of human umbilical cord blood mesenchymal stem cells (CD34-).
abstract: This study evaluated the effects of estrogen on the neuronal differentiation of human umbilical cord blood mesenchymal stem cells. Human umbilical cord blood mesenchymal stem cells cultured in a neuronal differentiation medium containing dimethylsulfoxide and butylated hydroxyanisole showed the expression of the neuronal cell-specific protein marker, beta-tubulin III. The estrogen treatment increased the proportion of neurons and neurite branching but reduced the mean neurite length. The relative expression of neurotropic factors such as brain-derived neurotropic factor, glial cell derived neurotropic factor, nerve growth factor, neurotrophin-3, and growth-associated protein 43 were higher in the estrogen-treated group than in the nontreated and estrogen receptor antagonist (ICI-182,780)-treated groups. These results suggest that estrogen stimulates the differentiation of neurons derived from human umbilical cord blood mesenchymal stem cells through the gene expression of neurotrophic factors.
authors.affiliation: School of Biotechnology and Biomedical Science, Inje University, Gimhae 621-749, Korea.
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Kang
authors.forename: Chae Kwan
authors.initials: CK
authors.lastname: Lee
authors.forename: Ju Ran
authors.initials: JR
authors.lastname: Kim
authors.forename: Seong Jin
authors.initials: SJ
authors.lastname: Yu
authors.forename: Jong Hee
authors.initials: JH
authors.lastname: Jo
authors.forename: Byung-Rok
authors.initials: BR
authors.lastname: Do
authors.forename: Hae Kwon
authors.initials: HK
authors.lastname: Kim
authors.forename: Sung Goo
authors.initials: SG
authors.lastname: Kang
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects*; D004347:Drug Interactions; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology; D004965:Estrogen Antagonists / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology*; D000077267:Fulvestrant; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D004965:Estrogen Antagonists; D004967:Estrogens; D009414:Nerve Growth Factors; D000077267:Fulvestrant; D004958:Estradiol