Metabolism of glutathione in tumour cells as evidenced by 1H MRS.
1H MRS signals of glutathione and of free glutamate were examined in samples from cultured tumour cells, namely MCF-7 from mammary carcinoma and TG98 from malignant glioma, with the aim of relating signal intensities to aspects of GSH metabolism. Spectra of cells harvested at different cell densities suggest that GSH and glu signal intensities are related to cell density and proliferation and their ratio is dependent on the activity of the gamma-glutamyl cysteine synthetase. The hypothesis is confirmed by experiments performed on cells treated with buthionine sulfoximine that inhibits the enzyme activity.
Grande
S
S
Dipartimento Tecnologie e Salute and INFN Gruppo Collegato Sanita', Istituto Superiore di Sanita', Viale Regina Elena 299, 00161 Roma, Italy.
Luciani
A M
AM
Rosi
A
A
Palma
A
A
Giovannini
C
C
Sapora
O
O
Guidoni
L
L
Viti
V
V
D019328:Buthionine Sulfoximine / Q000494:pharmacology; D045744:Cell Line, Tumor; D003545:Cysteine / Q000032:analysis; D018698:Glutamic Acid / Q000032:analysis; D005978:Glutathione / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology*; D011522:Protons; D013997:Time Factors
D011522:Protons; D018698:Glutamic Acid; D019328:Buthionine Sulfoximine; D005978:Glutathione; D003545:Cysteine