Defective responsiveness of CD5+ B1 cells to lipopolysaccharide in cytokine production.
Previously, we found that mouse TH2.52 cells possess the characteristic of CD5(+) B1 cells and proliferate in response to lipopolysaccharide (LPS). The effect of LPS on cytokine production by TH2.52 B1 cells was studied. TH2.52 cells constitutively produced a small amount of tumor necrosis factor (TNF)-alpha and interleukin (IL)-6, and TNF-alpha and IL-6 production was markedly enhanced by LPS stimulation. Although interferon (IFN)-gamma caused the production of various cytokines, such as IL-2, IL-4, IL-6 and TNF-alpha in TH2.52 cells, LPS did not cause the production of such cytokines. LPS did not induce IFN-beta production in TH2.52 cells and TH2.52 cells lacked the expression of several molecules participating in the MyD88-independent pathway in LPS signaling. Defective responsiveness of TH2.52 B1 cells to LPS in cytokine production might be responsible for the failure of IFN-beta production due to the lack of molecules participating in the MyD88-independent pathway.
Koide
Naoki
N
Department of Microbiology and Immunology, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan. koide@aichi-med-u.ac.jp
Morikawa
Akiko
A
Ito
Hiroyasu
H
Sugiyama
Tsuyoshi
T
Hassan
Ferdaus
F
Islam
Shamima
S
Tumurkhuu
Gantsetseg
G
Mori
Isamu
I
Yoshida
Tomoaki
T
Yokochi
Takashi
T
D000818:Animals; D015703:Antigens, CD / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D018956:CD5 Antigens / Q000276:immunology*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000633:toxicity*; D051379:Mice; D013154:Spleen / Q000276:immunology
D015703:Antigens, CD; D018956:CD5 Antigens; D016207:Cytokines; D008070:Lipopolysaccharides