title: Bone cell-materials interaction on Si microchannels with bioinert coatings.
abstract: Bone implant life is dependent upon integration of biomaterial surfaces with local osteoblasts. This investigation studied the effects of various microchannel parameters and surface chemistry on immortalized osteoblast precursor cell (OPC1) adhesion. Cell-materials interactions were observed within channels of varying length, width, tortuosity, convergence, divergence and chemistry. Si wafers were used to create four distinct 1cm(2) designs of varying channel dimensions. After anisotropic chemical etching to a depth of 120microm, wafers were sputter coated with gold and titanium; and on another surface SiO(2) was grown to vary the surface chemistry of these microchannels. OPC1 cells were seeded in the central cavity of each chip before incubation in tissue culture plates. On days 5, 11 and 16, samples were taken out, fixed and processed for microscopic analysis. Samples were visually characterized, qualitatively scored and analyzed. Channel walls did not contain OPC1 migration, but showed locally interrupted adhesion. Scores for channels of floor widths as narrow as 350microm were significantly reduced. No statistically significant preference was detected for gold, titanium or SiO(2) surfaces. Bands of OPC1 cells appeared to align with nearby channels, suggesting that cell morphology may be controlled by topography of the design to improve osseointegration.
authors.affiliation: W.M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164-2920, USA.
authors.forename: Russell
authors.initials: R
authors.lastname: Condie
authors.forename: Susmita
authors.initials: S
authors.lastname: Bose
authors.forename: Amit
authors.initials: A
authors.lastname: Bandyopadhyay
mesh_terms: D001842:Bone and Bones / Q000166:cytology*; D002448:Cell Adhesion; D002450:Cell Communication / Q000502:physiology*; D002461:Cell Line, Transformed; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D012822:Silicon Dioxide / Q000737:chemistry*; D013234:Stem Cells / Q000166:cytology; D013499:Surface Properties; D023822:Tissue Engineering; D014025:Titanium / Q000737:chemistry
chemical_list: D020099:Coated Materials, Biocompatible; D006046:Gold; D012822:Silicon Dioxide; D014025:Titanium