Determination of binding constant and binding region of programmed cell death 5-heparin by capillary zone electrophoresis.
A sensitive and selective high-performance analytical method based on capillary zone electrophoresis (CZE) was developed for investigating interactions between heparin and programmed cell death 5 (PDCD5) qualitatively and quantitatively. The binding constant of the interaction between PDCD5 and heparin calculated by Scatchard analysis was 4.17x10(4) M(-1) and the binding sites located in the C-terminal region of PDCD5 (residues 109-115). The precisions of migration times, peak heights and binding constants, expressed as the relative standard deviation, were less than 2.4%, 1.1% and 5.7%, respectively.
Yi
Liu
L
Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China.
Xiaomei
Ling
L
Hui
Fan
F
Yingcheng
Zhong
Z
Dan
Li
L
Ying
Wang
W
D000595:Amino Acid Sequence; D051017:Apoptosis Regulatory Proteins / Q000737:chemistry / Q000378:metabolism*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006493:Heparin / Q000378:metabolism*; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D012680:Sensitivity and Specificity
D051017:Apoptosis Regulatory Proteins; D009363:Neoplasm Proteins; C117283:PDCD5 protein, human; D006493:Heparin