Sherlock Holmes and the proteome--a detective story.
The performance of a hexapeptide ligand library in capturing the 'hidden proteome' is illustrated and evaluated. This library, insolubilized on an organic polymer and available under the trade name 'Equalizer Bead Technology', acts by capturing all components of a given proteome, by concentrating rare and very rare proteins, and simultaneously diluting the abundant ones. This results in a proteome of 'normalized' relative abundances, amenable to analysis by MS and any other analytical tool. Examples are given of analysis of human urine and serum, as well as cell and tissue lysates, such as Escherichia coli and Saccharomyces cerevisiae extracts. Another important application is impurity tracking and polishing of recombinant DNA products, especially biopharmaceuticals meant for human consumption.
Righetti
Pier Giorgio
PG
Department of Chemistry Materials and Chemical Engineering Giulio Natta, Polytechnic of Milano, Milan, Italy. piergiorgio.righetti@polimi.it
Boschetti
Egisto
E
D015415:Biomarkers / Q000737:chemistry; D001798:Blood Proteins / Q000032:analysis / Q000302:isolation & purification; D020650:Combinatorial Chemistry Techniques; D004274:DNA, Recombinant / Q000737:chemistry; D029968:Escherichia coli Proteins / Q000032:analysis / Q000302:isolation & purification; D006801:Humans; D008024:Ligands; D013058:Mass Spectrometry; D019151:Peptide Library; D020543:Proteome / Q000032:analysis* / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods*; D029701:Saccharomyces cerevisiae Proteins / Q000032:analysis / Q000302:isolation & purification; D014556:Urine / Q000737:chemistry
D015415:Biomarkers; D001798:Blood Proteins; D004274:DNA, Recombinant; D029968:Escherichia coli Proteins; D008024:Ligands; D019151:Peptide Library; D020543:Proteome; D029701:Saccharomyces cerevisiae Proteins