Establishment and characterization of a cell line (NABCA) derived from metastatic lymph nodes of breast scirrhous carcinoma.
We successfully established a breast scirrhous carcinoma cell line (designated as NABCA) derived from metastatic tumors of the lymph node. The cells grew as multi-layered cultures without contact inhibition. The population doubling time was approximately 66 h. G-band karyotype of NABCA revealed 66% diploid, XX. Surprisingly, the cells had a number of secretory granules and straight microvilli as a brash border. In heterotransplantation, the cells produced a tumor resembling the original tumor. The NABCA is sensitive to Adriamycin (doxorubicin; KYOWA HAKKO KOGYO, Tokyo, Japan) and Taxol (paclitaxel; Bristol-Myers KK, Tokyo, Japan). This cell line is useful for studying the mechanism of lymphatic metastasis and susceptibility of anticancer drugs in human breast scirrhous cancer.
Ohi
Satoshi
S
Department of Anatomy II, Jikei University School of Medicine, Tokyo, Japan. satoshi-ohi@jikei.ac.jp
Kyoda
Shigeya
S
Tabei
Isao
I
Ninomiya
Kouzou
K
Sugiyama
Kayoko
K
Hashimoto
Hisashi
H
Tachibana
Toshiaki
T
Ishikawa
Hiroshi
H
D002293:Adenocarcinoma, Scirrhous / Q000235:genetics / Q000473:pathology* / Q000556:secondary / Q000648:ultrastructure; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology* / Q000648:ultrastructure; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm; D005260:Female; D006801:Humans; D007621:Karyotyping; D008207:Lymphatic Metastasis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D008875:Middle Aged; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous
D000970:Antineoplastic Agents