Rapid and sensitive liquid chromatography-tandem mass spectrometry method for the quantitation of colchicine in human plasma.
A rapid and sensitive method to determine colchicine in human plasma by liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed. Colchicine and the internal standard (I.S.), tegafur, were extracted from the matrix with n-hexane:dichloromethane:isopropanol (300:150:15, v/v/v) and separated by reversed-phase high-performance liquid chromatography (HPLC) using formic acid:10 mM ammonium acetate:methanol (1:49:75, v/v/v) as the mobile phase in a run time of 2.5 min. Detection was carried out by electrospray positive ionization mass spectrometry in the multiple-reaction monitoring (MRM) mode. The assay was linear in the concentration range 0.050-10 ng/ml with intra- and inter-day precision (as relative standard deviation (R.S.D.)) of <2 and <7%, respectively. The method was applied to a pharmacokinetic study of colchicine in healthy volunteers given an oral dose of 2.0 mg.
Jiang
Yao
Y
Research Center for Drug Metabolism, College of Life Science, Jilin University, 130023 Changchun, China.
Wang
Jiang
J
Wang
Yingwu
Y
Li
Hao
H
Fawcett
J Paul
JP
Gu
Jingkai
J
D000328:Adult; D000893:Anti-Inflammatory Agents / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003078:Colchicine / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D008297:Male; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000893:Anti-Inflammatory Agents; D003078:Colchicine