Altered HDAC5 and CREB mRNA expressions in the peripheral leukocytes of major depression.
BACKGROUND
Gene expressions of the peripheral leukocytes in depressive patients might reflect the systemic dysfunction of major depression. We determined mRNA expression levels of Histone deacetylase 5 (HDAC5) gene and cyclic AMP response element-binding protein 1 (CREB) gene in the leukocyte of depressive patients. HDAC5 and CREB are reported to be important targets of antidepressants, the latter being located in the downstream of the former in lymphocyte calcium signaling.


METHODS
25 patients with major depression and 25 age- and sex-matched healthy controls were included in this study. Twenty patients were able to be followed up until the 8 week-treatment. The mRNA levels were determined by a quantitative RT-PCR method.


RESULTS
Levels of HDAC5 and CREB mRNA were significantly higher in drug-free depressive patients than those of controls and the higher mRNA levels decreased to control levels after 8-week paroxetine treatment. There were positive correlation between levels of HDAC5 and CREB.


CONCLUSIONS
Our results suggest the alteration of HDAC5 and CREB gene expression in the systemic pathophysiology of major depression.
Iga
Jun-Ichi
J
Department of Psychiatry, Course of Integrated Brain Sciences, University of Tokushima School of Medicine, Tokushima 770-8503, Japan.
Ueno
Shu-Ichi
S
Yamauchi
Ken
K
Numata
Shusuke
S
Kinouchi
Sawako
S
Tayoshi-Shibuya
Sumiko
S
Song
Hongwei
H
Ohmori
Tetsuro
T
D000328:Adult; D000368:Aged; D050882:CREB-Binding Protein / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D003865:Major Depressive Disorder / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D017374:Paroxetine / Q000097:blood; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018709:Statistics, Nonparametric
D012333:RNA, Messenger; D017374:Paroxetine; D050882:CREB-Binding Protein; C494363:CREBBP protein, human; C404500:HDAC5 protein, human; D006655:Histone Deacetylases