title: The contribution of B cells to renal interstitial inflammation.
abstract: Local B-cell infiltrates play a role in tissue fibrosis, neolymphangiogenesis, and renal allograft survival. We sought to characterize the B-cell infiltrates, factors involved in B-cell recruitment, and lymphangiogenesis in renal interstitial injury (ie, acute and chronic interstitial nephritis and chronic IgA nephropathy). CD20-positive B cells formed a prominent part of the interstitial infiltrating cells. Together with CD3-positive T cells, the CD20-positive B cells formed larger nodular structures. CD10-positive pre-B cells were rare, and the majority were mature CD27-positive B cells. Proliferating B cells were detected within nodular infiltrates. The level of mRNA expression of the chemokine CXCL13 was increased and correlated with CD20 mRNA in the tubulointerstitial space. CXCL13 protein was predominantly found at sites of nodular infiltrates, in association with CXCR5-positive B cells. Furthermore, sites of chronic interstitial inflammation were associated with a high number of lymphatic vessels. B-cell infiltrates form a prominent part of the interstitial infiltrates both in primary interstitial lesions and in IgA nephropathy. CXCR5-positive B cells might be recruited via the chemokine CXCL13 and seem to contribute to the formation of intrarenal lymphoid follicle-like structures. These might represent an intrarenal immune system.
authors.affiliation: Medizinische Poliklinik-Innenstadt, University of Munich, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Heller
authors.forename: Maja T
authors.initials: MT
authors.lastname: Lindenmeyer
authors.forename: Clemens D
authors.initials: CD
authors.lastname: Cohen
authors.forename: Ulrike
authors.initials: U
authors.lastname: Brandt
authors.forename: Dan
authors.initials: D
authors.lastname: Draganovici
authors.forename: Michael
authors.initials: M
authors.lastname: Fischereder
authors.forename: Matthias
authors.initials: M
authors.lastname: Kretzler
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Anders
authors.forename: Thomas
authors.initials: T
authors.lastname: Sitter
authors.forename: Isabella
authors.initials: I
authors.lastname: Mosberger
authors.forename: Dontscho
authors.initials: D
authors.lastname: Kerjaschki
authors.forename: Heinz
authors.initials: H
authors.lastname: Regele
authors.forename: Detlef
authors.initials: D
authors.lastname: Schlöndorff
authors.forename: Stephan
authors.initials: S
authors.lastname: Segerer
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology* / Q000473:pathology; D054382:Chemokine CXCL13; D019743:Chemokines, CXC / Q000276:immunology; D002648:Child; D002908:Chronic Disease; D005260:Female; D005922:Glomerulonephritis, IGA / Q000276:immunology* / Q000473:pathology; D006801:Humans; D042601:Lymphatic Vessels / Q000276:immunology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009395:Nephritis, Interstitial / Q000276:immunology* / Q000473:pathology; D054380:Receptors, CXCR5; D019707:Receptors, Chemokine; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology
chemical_list: D015703:Antigens, CD; C110663:CXCL13 protein, human; C095085:CXCR5 protein, human; D054382:Chemokine CXCL13; D019743:Chemokines, CXC; D054380:Receptors, CXCR5; D019707:Receptors, Chemokine