Novel membrane-localizing TEMPO derivatives for measurement of cellular oxidative stress at the cell membrane.
Oxidative stress affecting lipid membranes is considered to be closely related to cardiovascular disease and brain ischemia. In this study, we designed and synthesized membrane-localizing TEMPO derivatives and demonstrated that one of these synthesized probes, compound 1, localized and detected oxidative stress in the cell membrane in an endotoxic model of a mouse macrophage-like cell line. Compound 1 is therefore a potentially useful probe for evaluating oxidative stress at the cell membrane.
Ban
Shizuka
S
Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya, Aichi 467-8603, Japan.
Nakagawa
Hidehiko
H
Suzuki
Takayoshi
T
Miyata
Naoki
N
D000818:Animals; D002545:Brain Ischemia / Q000473:pathology; D002318:Cardiovascular Diseases / Q000473:pathology; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D003497:Cyclic N-Oxides / Q000138:chemical synthesis*; D015195:Drug Design; D004731:Endotoxins; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D018384:Oxidative Stress; D013113:Spin Labels / Q000138:chemical synthesis*; D013329:Structure-Activity Relationship
D003497:Cyclic N-Oxides; D004731:Endotoxins; D013113:Spin Labels; C003959:TEMPO