title: Detection of rabies virus genes by in-situ polymerase chain reaction.
abstract: Rabies is diagnosed by FAT in the impression smears of brain tissues. In this study, an attempt was made to diagnose rabies using in situ polymerase chain reaction (ISPCR). A digoxigenin-labelled double-stranded probe specific for a portion of the 'N' gene of rabies virus was used. Positive signals were identified as blue dots in the intraneuronal and neuropil areas.
authors.affiliation: Indian Veterinary Research Institute, Izatnagar, Bareilly.
authors.forename: P Ezhil
authors.initials: PE
authors.lastname: Praveena
authors.forename: R
authors.initials: R
authors.lastname: Jayakumar
authors.forename: C
authors.initials: C
authors.lastname: Balachandran
authors.forename: G
authors.initials: G
authors.lastname: Thirumurugan
authors.forename: G Dhinakar
authors.initials: GD
authors.lastname: Raj
authors.forename: B Murali
authors.initials: BM
authors.lastname: Manohar
mesh_terms: D000818:Animals; D002493:Central Nervous System Diseases / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D004283:Dog Diseases / Q000175:diagnosis / Q000821:virology*; D004285:Dogs; D005260:Female; D006624:Hippocampus / Q000821:virology; D007150:Immunohistochemistry / Q000662:veterinary; D008297:Male; D019590:Nucleocapsid Proteins / Q000737:chemistry / Q000235:genetics; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D011818:Rabies / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D011820:Rabies virus / Q000235:genetics* / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D013687:Telencephalon / Q000821:virology
chemical_list: D019590:Nucleocapsid Proteins; D012367:RNA, Viral