[Ribosomal DNA ITS sequence analyses of Pinellia ternata from different geographical origin in China].
OBJECTIVE
To study the ITS sequence variation of Pinellia ternata from different population in China, and it correlation to geographical distribution and morpha of the plant.


METHODS
The rDNA ITS regions of various P. ternata were amplified and sequenced. And they were analyzed by means of the software of CLUSTRAL and MEGA.


RESULTS
Complete sequence of ITS and 5.8S rDNA of 16 different P. ternata population were obtained. The sequences of ITS1, 5.8S and ITS2 are 276,162 and 246 bp, respectively. ITS1 was more conservative than ITS2. Phylogenetic tree based on ITS1 and ITS2 sequences data was conducted by Neighbor-joining method.


CONCLUSIONS
Ribosomal DNA ITS sequence analyses can be applied to the resource research of P. ternata.
Zhang
Jun-Yi
JY
Institute of Chinese Medicinal Materials, Nanjing Agriculture University, Nanjing 210095, China.
Guo
Qiao-Sheng
QS
Wu
Li-Wei
LW
Hang
Yue-Yu
YY
D001483:Base Sequence; D002681:China; D018744:DNA, Plant / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D017753:Ecosystem; D008969:Molecular Sequence Data; D010802:Phylogeny; D030021:Pinellia / Q000235:genetics*; D035281:Plant Tubers / Q000235:genetics; D010946:Plants, Medicinal / Q000235:genetics*; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D017422:Sequence Analysis, DNA
D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer; D012340:RNA, Ribosomal, 5.8S