Carbenoxolone and triterpenoids inhibited mucin secretion from airway epithelial cells.
This study investigated whether carbenoxolone, oleanolic acid and ursolic acid affect ATP-induced mucin secretion from cultured airway epithelial cells. Confluent primary hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled using (3)H-glucosamine for 24 h and chased for 30 min in the presence of varying concentrations of each agent to assess the effects on (3)H-mucin secretion. The possible cytotoxicity of each agent was investigated with a lactate dehydrogenase assay. The results were as follows: (1) carbenoxolone, oleanolic acid and ursolic acid significantly inhibited the secretion of airway mucin induced by ATP; (2) none of the compounds showed significant cytotoxicity at any concentration. This result suggests that carbenoxolone, oleanolic acid and ursolic acid can regulate 'mucin secretion induced by ATP'--phenomenon simulating mucus overproduction from inflamed airway epithelial cells--y directly acting on airway mucin-secreting cells.
Heo
Ho Jin
HJ
Department of Pharmacology, College of Medicine, Chungnam National University, Daejeon, Korea.
Kim
Cheolsu
C
Lee
Hyun Jae
HJ
Kim
Young Sik
YS
Kang
Sam Sik
SS
Seo
Un Kyo
UK
Kim
Yun Hee
YH
Park
Yang Chun
YC
Seok
Jeong Ho
JH
Lee
Choong Jae
CJ
D000255:Adenosine Triphosphate / Q000378:metabolism / Q000502:physiology; D000818:Animals; D002229:Carbenoxolone / Q000494:pharmacology*; D002478:Cells, Cultured; D006224:Cricetinae; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D009077:Mucins / Q000378:metabolism*; D009828:Oleanolic Acid / Q000494:pharmacology*; D020545:Respiratory Mucosa / Q000187:drug effects* / Q000378:metabolism; D014315:Triterpenes / Q000494:pharmacology*; D000097245:Ursolic Acid
D000255:Adenosine Triphosphate; D002229:Carbenoxolone; D009077:Mucins; D009828:Oleanolic Acid; D014315:Triterpenes; D000097245:Ursolic Acid