Nitrification Inhibitors from the root tissues of Brachiaria humidicola, a tropical grass.
Nitrification inhibitory activity was found in root tissue extracts of Brachiaria humidicola, a tropical pasture grass. Two active inhibitory compounds were isolated by activity-guided fractionation, using recombinant Nitrosomonas europaea containing luxAB genes derived from the bioluminescent marine gram-negative bacterium Vibrio harveyi. The compounds were identified as methyl-p-coumarate and methyl ferulate, respectively. Their nitrification inhibitory properties were confirmed in chemically synthesized preparations of each. The IC50 values of chemically synthesized preparations were 19.5 and 4.4 microM, respectively. The ethyl, propyl, and butyl esters of p-coumaric and ferulic acids inhibited nitrification, whereas the free acid forms did not show inhibitory activity.
Gopalakrishnan
Subramaniam
S
Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, Tsukuba, Ibaraki 305-8686, Japan.
Subbarao
Guntur V
GV
Nakahara
Kazuhiko
K
Yoshihashi
Tadashi
T
Ito
Osamu
O
Maeda
Ikuko
I
Ono
Hiroshi
H
Yoshida
Mitsuru
M
D000641:Ammonia / Q000378:metabolism; D003373:Coumaric Acids / Q000494:pharmacology; D008745:Methylation; D009566:Nitrates / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D009584:Nitrogen / Q000037:antagonists & inhibitors* / Q000378:metabolism; D009606:Nitrosomonas / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry*; D006109:Poaceae / Q000737:chemistry*; D011422:Propionates; D014733:Vibrio / Q000235:genetics / Q000378:metabolism
D003373:Coumaric Acids; D009566:Nitrates; D009573:Nitrites; D011422:Propionates; D000641:Ammonia; C004999:ferulic acid; C495469:p-coumaric acid; D009584:Nitrogen