Bone biopsy derived marrow stromal elements rescue chronic lymphocytic leukemia B-cells from spontaneous and drug induced cell death and facilitates an "angiogenic switch".
A novel bone biopsy technique was used to generate a robust stromal cell system to study how stroma modulates CLL B-cell apoptosis and how the leukemic cell-stromal interaction influences secretion of vascular factors. Marrow stromal elements (MSE) rescued CLL B-cells from both spontaneous and drug induced apoptosis, partly due to soluble factors. When CLL B-cells were added to the MSE cultures, a dramatic increase in the secretion of basic fibroblast growth factor and decrease in the secretion of thrombospondin was observed. These results indicate the interaction between CLL B-cells and marrow stromal elements regulates angiogenic switching and may be linked to disease progression.
Kay
Neil E
NE
Mayo Clinic, Stabile 628, 200 First Street SW, Rochester, MN 55905, USA. kay.neil@mayo.edu
Shanafelt
Tait D
TD
Strege
Ann K
AK
Lee
Yean K
YK
Bone
Nancy D
ND
Raza
Azra
A
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001707:Biopsy, Needle; D001854:Bone Marrow Cells / Q000473:pathology*; D002450:Cell Communication; D002699:Chlorambucil / Q000494:pharmacology*; D018920:Coculture Techniques; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000473:pathology*; D017154:Stromal Cells / Q000473:pathology*; D019699:Thrombospondins / Q000378:metabolism
D018906:Antineoplastic Agents, Alkylating; D019699:Thrombospondins; D016222:Fibroblast Growth Factor 2; D002699:Chlorambucil