title: Global changes in the hippocampal proteome following exposure to an enriched environment.
abstract: Exposure to an enriched environment promotes neurochemical, structural and neurophysiological changes in the brain and is associated with enhanced synaptic plasticity and improved hippocampal-dependent learning. Using a global proteomics-based approach we have now been able to reveal the altered expression of a diverse range of hippocampal proteins following exposure to an enriched environment. Male Hooded Lister rats (8 weeks) were subjected to a 6-week regimen in which they were housed in either non-enriched (open field) or enriched conditions (toys, wheels etc.). Whole protein extracts from stratum pyramidale and stratum radiatum of area CA1 were then isolated and subjected to differential gel electrophoresis [McNair K, Davies CH, Cobb SR (2006) Plasticity-related regulation of the hippocampal proteome. Eur J Neurosci 23(2):575-580]. Of the 2469 resolvable protein spots detected in this study, 42 spots (1.7% of the detectable proteome) derived from predominantly somatic fractions and 32 proteins spots from dendritic fractions (1.3% of detectable proteome) were significantly altered in abundance following exposure to an enriched environment (somatic: 14 increased/28 decreased abundance, range -1.5 to +1.4-fold change; dendritic: 16 increased, 16 decreased abundance, range -1.6 to +3.0-fold change). Following in-gel tryptic digestion and Maldi-Tof/Q-star mass spectrometry, database searching revealed the identity of 50 protein spots displaying environmental enrichment-related modulation of expression. Identified proteins belonged to a variety of functional classes with gene ontology analysis revealing the majority (>70%) of regulated proteins to be part of the energy metabolism, cytoplasmic organization/biogenesis and signal transduction processes.
authors.affiliation: Division of Neuroscience and Biomedical Systems, IBLS, University of Glasgow, Glasgow, G12 8QQ, UK.
authors.forename: K
authors.initials: K
authors.lastname: McNair
authors.forename: J
authors.initials: J
authors.lastname: Broad
authors.forename: G
authors.initials: G
authors.lastname: Riedel
authors.forename: C H
authors.initials: CH
authors.lastname: Davies
authors.forename: S R
authors.initials: SR
authors.lastname: Cobb
mesh_terms: D000818:Animals; D003712:Dendrites / Q000378:metabolism / Q000648:ultrastructure; D004734:Energy Metabolism / Q000502:physiology; D004779:Environment Design; D005106:Exploratory Behavior / Q000502:physiology; D015870:Gene Expression / Q000502:physiology*; D006624:Hippocampus / Q000378:metabolism*; D007858:Learning / Q000502:physiology; D008297:Male; D008568:Memory / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000378:metabolism*; D009473:Neuronal Plasticity / Q000502:physiology*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D017966:Pyramidal Cells / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D015398:Signal Transduction / Q000502:physiology; D015854:Up-Regulation / Q000502:physiology
chemical_list: D009419:Nerve Tissue Proteins; D020543:Proteome