title: 1H MRS signals from glutathione may act as predictive markers of apoptosis in irradiated tumour cells.
abstract: Inhibition of apoptosis in tumour cells may depend on intracellular reduced glutathione (GSH) level. In this work, GSH levels were studied by (1)H MRS in MCF-7 and HeLa cells, characterised by a different radiosensitivity. Annexin-V test showed that the fraction of apoptotic HeLa cells after irradiation is much higher than in control, although MCF-7 cells did not show a significant apoptosis. MRS signals from GSH (G) show lower intensity in HeLa with respect to MCF-7 cells; the opposite is true for free glutamic acid [glu (g)]. After irradiation, the G/g ratio decreases in MCF-7, although remaining approximately constant in HeLa cells. Buthionine sulfoximine (BSO) treated MCF-7 cells show an increase in the percentage of apoptotic cells; in parallel, G/g ratio behaves as in HeLa. This study indicates that GSH level may act as predictive marker of apoptosis by irradiation.
authors.affiliation: Department of Technology and Health and INFN Sanità Group, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Roma, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Grande
authors.forename: C
authors.initials: C
authors.lastname: Giovannini
authors.forename: L
authors.initials: L
authors.lastname: Guidoni
authors.forename: A M
authors.initials: AM
authors.lastname: Luciani
authors.forename: A
authors.initials: A
authors.lastname: Palma
authors.forename: A
authors.initials: A
authors.lastname: Rosi
authors.forename: O
authors.initials: O
authors.lastname: Sapora
authors.forename: V
authors.initials: V
authors.lastname: Viti
mesh_terms: D017209:Apoptosis / Q000528:radiation effects*; D014408:Biomarkers, Tumor / Q000032:analysis*; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D004307:Dose-Response Relationship, Radiation; D005978:Glutathione / Q000032:analysis*; D006367:HeLa Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011522:Protons; D011829:Radiation Dosage; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D011522:Protons; D005978:Glutathione