title: [Changes of apoptosis, mitochondrion membrane potential and Ca2+ of hypothalamic neurons induced by high power microwave].
abstract: OBJECTIVE
  To explore the injury effect and mechanism of hypothalamic neurons after high power microwave (HPM) exposure.
  
  
  METHODS
  Primarily cultured hypothalamic neurons were exposed to 10 and 30 mW/cm(2) HPM, and the inverted phase contrast microscope (IPCM) and flow cytometry (FCM) were employed to detect the injury of cells and change of mitochondrion membrane potential (MMP) and Ca(2+) in the cytoplasm of neurons.
  
  
  RESULTS
  The ratio of apoptosis was significantly higher than that of the sham exposure (P < 0.05) induced by 10 and 30 mW/cm(2) HPM and necrosis increased significantly (P < 0.05) in the group of 30 mW/cm(2) at 6 h after exposure. The content of Ca(2+) in the cytoplasm of neuron cells increased (P < 0.01) while MMP decreased significantly (P < 0.01) after radiation of 30 mW/cm(2) HPM at 6 h after exposure.
  
  
  CONCLUSIONS
  Apoptosis is one of the major death ways of hypothalamic neurons. The overloading of Ca(2+) and the decline of MMP are involved in the process.
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Li
authors.initials: L
authors.lastname: Meng
authors.forename: Rui-Yun
authors.initials: RY
authors.lastname: Peng
authors.forename: Ya-Bing
authors.initials: YB
authors.lastname: Gao
authors.forename: Shui-Ming
authors.initials: SM
authors.lastname: Wang
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Ma
authors.forename: Wen-Hua
authors.initials: WH
authors.lastname: Hu
authors.forename: De-Wen
authors.initials: DW
authors.lastname: Wang
authors.forename: Zhen-Tao
authors.initials: ZT
authors.lastname: Su
authors.forename: Bo
authors.initials: B
authors.lastname: Dong
authors.forename: Tian-Hao
authors.initials: TH
authors.lastname: Xu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000528:radiation effects*; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D007031:Hypothalamus / Q000166:cytology / Q000528:radiation effects; D053078:Membrane Potential, Mitochondrial / Q000528:radiation effects*; D008564:Membrane Potentials; D008872:Microwaves / Q000009:adverse effects*; D009474:Neurons / Q000378:metabolism* / Q000528:radiation effects*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002118:Calcium