Increased midkine gene expression in childhood B-precursor acute lymphoblastic leukemia.
Midkine (MK) is a heparin-binding growth factor that is overexpressed in a number of solid cancers. However, expression in acute leukemia has not been clarified. We examined MK gene expression using real-time PCR in 94 children with acute leukemia. In 30 of the 41 patients with B-precursor ALL, MK gene expression was overexpressed than normal BM. MK gene was also overexpressed in more than half of patients with FAB M1 and M2 types of AML. Quantification of MK gene by real-time PCR offers particular promise as a prognostic marker and a marker for minimal residual disease in children with B-precursor ALL.
Hidaka
Hirokazu
H
Department of Pediatrics, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Yagasaki
Hiroshi
H
Takahashi
Yoshiyuki
Y
Hama
Asahito
A
Nishio
Nobuhiro
N
Tanaka
Makito
M
Yoshida
Nao
N
Villalobos
Itzel Bustos
IB
Wang
Yue
Y
Xu
Yinyan
Y
Horibe
Keizo
K
Chen
Sen
S
Kadomatsu
Kenji
K
Kojima
Seiji
S
D000293:Adolescent; D015153:Blotting, Western; D001853:Bone Marrow / Q000378:metabolism / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D005260:Female; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D015448:Leukemia, B-Cell / Q000235:genetics* / Q000378:metabolism; D007951:Leukemia, Myeloid / Q000235:genetics* / Q000378:metabolism; D008297:Male; D000078792:Midkine; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D009414:Nerve Growth Factors / Q000235:genetics* / Q000378:metabolism; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
C066254:MDK protein, human; D009363:Neoplasm Proteins; D009414:Nerve Growth Factors; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D000078792:Midkine