Huperzine A protects C6 rat glioma cells against oxygen-glucose deprivation-induced injury.
The protective effects of huperzine A against oxygen-glucose deprivation (OGD)-induced injury in C6 cells were investigated. OGD for 6h and reoxygenation for 6h enhanced phosphorylation and degradation of IkappaBalpha and nuclear translocation of nuclear factor-kappa B (NF-kappaB), triggered overexpression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and nitric oxide (NO) in C6 cells. Along with inhibiting acetylcholinesterase activity, treatment with 1 microM huperzine A inhibited activation of NF-kappaB, attenuated iNOS, COX-2 and NO overexpression, and promoted survival in C6 cells subjected to OGD insult. The protective effects of huperzine A were partly mediated by "cholinergic anti-inflammatory pathway" through alpha7 nicotinic acetylcholine receptor.
Wang
Zhi Fei
ZF
State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, People's Republic of China.
Tang
Xi Can
XC
D000110:Acetylcholinesterase / Q000378:metabolism; D000470:Alkaloids; D000818:Animals; D002038:Bungarotoxins / Q000494:pharmacology; D015687:Cell Hypoxia / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000378:metabolism; D005910:Glioma / Q000201:enzymology / Q000473:pathology*; D005947:Glucose / Q000172:deficiency*; D020672:I-kappa B Proteins / Q000378:metabolism; D008464:Mecamylamine / Q000494:pharmacology; D000072000:NF-KappaB Inhibitor alpha; D009569:Nitric Oxide / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D011392:Proline / Q000031:analogs & derivatives / Q000494:pharmacology; D021381:Protein Transport / Q000187:drug effects; D051381:Rats; D012717:Sesquiterpenes / Q000494:pharmacology*; D013859:Thiocarbamates / Q000494:pharmacology; D051996:Transcription Factor RelA / Q000378:metabolism
D000470:Alkaloids; D002038:Bungarotoxins; D020672:I-kappa B Proteins; C000606206:Nfkbia protein, rat; D012717:Sesquiterpenes; D013859:Thiocarbamates; D051996:Transcription Factor RelA; C050426:huperzine A; C066229:prolinedithiocarbamate; D000072000:NF-KappaB Inhibitor alpha; D009569:Nitric Oxide; D008464:Mecamylamine; D011392:Proline; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; D000110:Acetylcholinesterase; D005947:Glucose