title: The in vitro osteoclastic degradation of nacre.
abstract: Osteoclast activity was studied on nacre, the mother of pearl (MOP) in order to assess the plasticity of bone resorbing cells and their capacity to adapt to a biomineralized material with a different organic and mineral composition from that of its natural substrate, bone. Pure MOP, a natural biomineralized CaCO(3) material, was obtained from Pinctada oyster shell. When implanted in the living system, nacre has proven to be a sustainable bone grafting material although a limited surface degradation process. Osteoclast stem cells and mature osteoclasts were cultured on MOP substrate and osteoclast precursor cells were shown to differentiate into osteoclasts capable of resorbing nacre substrate. However, analysis of the organization of the cytoskeleton showed that both a sealing zone and a podosome structure were observed on the nacre substrate. Moreover, MOP resorption efficiency was consistently found to be lower than that of bone and appeared to be a limited process.
authors.affiliation: Département Milieux et Peuplements Aquatiques USM 401, UMR/CNRS 5178 BOME, Muséum National d'Histoire Naturelle, 43, rue Cuvier, 75231 Paris cedex 05, France. duplat@mnhn.fr
authors.forename: D
authors.initials: D
authors.lastname: Duplat
authors.forename: A
authors.initials: A
authors.lastname: Chabadel
authors.forename: M
authors.initials: M
authors.lastname: Gallet
authors.forename: S
authors.initials: S
authors.lastname: Berland
authors.forename: L
authors.initials: L
authors.lastname: Bédouet
authors.forename: M
authors.initials: M
authors.lastname: Rousseau
authors.forename: S
authors.initials: S
authors.lastname: Kamel
authors.forename: C
authors.initials: C
authors.lastname: Milet
authors.forename: P
authors.initials: P
authors.lastname: Jurdic
authors.forename: M
authors.initials: M
authors.lastname: Brazier
authors.forename: E
authors.initials: E
authors.lastname: Lopez
mesh_terms: D000818:Animals; D002119:Calcium Carbonate / Q000737:chemistry*; D002448:Cell Adhesion; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000648:ultrastructure; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010010:Osteoclasts / Q000166:cytology*; D011817:Rabbits
chemical_list: D002119:Calcium Carbonate