title: Identification of Burkholderia pseudomallei and related bacteria by multiple-locus sequence typing-derived PCR and real-time PCR.
abstract: Close relatedness and genomic plasticity characterizing the high-threat pathogens Burkholderia pseudomallei and Burkholderia mallei render the molecular diagnosis of these species hard to guarantee with a maximal confidence level. This article describes fast molecular assays derived from compiled sequences of housekeeping genes determined in more than 1,000 strains. The assays proved to be robust and appropriate for general detection as well as species identification purposes.
authors.affiliation: Department of Bacteriology and Immunology, Veterinary and Agrochemical Research Centre, Groeselenberg 99, 1180 Brussels, Belgium. Pierre.Wattiau@var.fgov.be
authors.forename: Pierre
authors.initials: P
authors.lastname: Wattiau
authors.forename: Mieke
authors.initials: M
authors.lastname: Van Hessche
authors.forename: Heinrich
authors.initials: H
authors.lastname: Neubauer
authors.forename: Reena
authors.initials: R
authors.lastname: Zachariah
authors.forename: Ulrich
authors.initials: U
authors.lastname: Wernery
authors.forename: Hein
authors.initials: H
authors.lastname: Imberechts
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D019117:Burkholderia / Q000145:classification* / Q000235:genetics; D042726:Burkholderia mallei / Q000145:classification* / Q000235:genetics; D016957:Burkholderia pseudomallei / Q000145:classification* / Q000235:genetics; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial