title: Campylobacter coli isolates derived from chickens in Senegal: Diversity, genetic exchange with Campylobacter jejuni and quinolone resistance.
abstract: We used the multilocus sequence typing (MLST) method to study the genetic diversity of Campylobacter coli isolated from chickens in Senegal, and to check the presence of genetic exchange with Campylobacter jejuni. In addition, we assessed the resistance of the isolates to ciprofloxacin and nalidixic acid, and their gyrA sequences. MLST revealed a low level of diversity and the absence of lineages among C. coli isolates. In addition, an exchange of alleles with C. jejuni was found. Twenty percent of the ciprofloxacin-resistant isolates lacked mutations within the quinolone resistance-determining region (QRDR) of GyrA. There was no link between quinolone resistance and sequence type (ST).
authors.affiliation: Laboratoire de Biologie médicale, Institut Pasteur de Dakar, 36, avenue Pasteur, Dakar, Sénégal. kinana@pasteur.sn
authors.forename: Alfred Dieudonné
authors.initials: AD
authors.lastname: Kinana
authors.forename: Eric
authors.initials: E
authors.lastname: Cardinale
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Bahsoun
authors.forename: Fatou
authors.initials: F
authors.lastname: Tall
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Sire
authors.forename: Sebastien
authors.initials: S
authors.lastname: Breurec
authors.forename: Benoit
authors.initials: B
authors.lastname: Garin
authors.forename: Cheikh
authors.initials: C
authors.lastname: Saad-Bouh Boye
authors.forename: Jean-David
authors.initials: JD
authors.lastname: Perrier-Gros-Claude
mesh_terms: D000483:Alleles; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D017000:Campylobacter coli / Q000187:drug effects* / Q000235:genetics*; D016123:Campylobacter jejuni / Q000235:genetics; D002645:Chickens / Q000382:microbiology*; D002939:Ciprofloxacin / Q000494:pharmacology; D027081:DNA Gyrase / Q000235:genetics; D024881:Drug Resistance, Bacterial; D014644:Genetic Variation; D009268:Nalidixic Acid / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D015363:Quinolones / Q000494:pharmacology*; D012675:Senegal
chemical_list: D000890:Anti-Infective Agents; D015363:Quinolones; D009268:Nalidixic Acid; D002939:Ciprofloxacin; D027081:DNA Gyrase