title: [Therapy of severe chondral defects of the patella by autologous chondrocyte implantation].
abstract: OBJECTIVE
  The aim of this study was to present the results of treatment of deep chondral defects of the patella by transplantation of a solid chondral graft formed by autologous cultured chondrocytes.
  
  
  METHODS
  Indications for autologous chondrocyte implantation most frequently included acute trauma to the knee, particularly the patella, which resulted in a severe focal cartilage defect. The lesions were assessed on the basis of the Bessette and Hunter classification preoperatively, and on the Outerbridge classification during arthroscopy. A sample of healthy cartilage was harvested from a non-weight-bearing area of the trochlea femoris. After cultivation of chondrocytes for 28 to 42 days in the tissue bank, a solid chondral graft, prepared with the use of fibrin glue (Tissucol), was ready for implantation.
  
  
  RESULTS
  Six patients, two males and four females (average age, 21.1 years; range, 13 to 39 years) were treated by this method in the period from July 2003 to October 2005. Follow-up ranged from 1 to 25 months, with an average of 18.5 months. The patients were examined postoperatively by magnetic resonance imaging (MRI) at 2 weeks, 2 and 6 months, and at 1 year. The clinical results were evaluated by the Meyers, the Tegner and the Lysholm knee scoring systems. Two of the six patients, in whom the follow-up period was shorter than 6 months, were not included in the evaluation.
  
  
  CONCLUSIONS
  A significant improvement in knee function was recorded when the preoperative and final follow-up stages were compared, as well as on comparison with the healthy contralateral knee joint. MRI examination showed good graft incorporation in all patients.
authors.affiliation: Ortopedická Klinika Detí a Dospelých 2. LF UK a FN Motol, Praha. milan.handl@lfmotol.cuni.cz
authors.forename: M
authors.initials: M
authors.lastname: Handl
authors.forename: T
authors.initials: T
authors.lastname: Trc
authors.forename: M
authors.initials: M
authors.lastname: Hanus
authors.forename: E
authors.initials: E
authors.lastname: Stastný
authors.forename: M
authors.initials: M
authors.lastname: Fricová-Poulová
authors.forename: J
authors.initials: J
authors.lastname: Neuwirth
authors.forename: J
authors.initials: J
authors.lastname: Adler
authors.forename: D
authors.initials: D
authors.lastname: Havranová
authors.forename: F
authors.initials: F
authors.lastname: Varga
mesh_terms: D000293:Adolescent; D000328:Adult; D002358:Cartilage, Articular / Q000293:injuries*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000637:transplantation*; D005260:Female; D006801:Humans; D008297:Male; D010329:Patella / Q000293:injuries*; D023822:Tissue Engineering; D014182:Transplantation, Autologous