Dynamic kinetic capillary isoelectric focusing: a powerful tool for studying protein-DNA interactions.
A new method called dynamic kinetic capillary isoelectric focusing (DK-CIEF) is presented for the study of protein-DNA interactions. The method is based on CIEF with laser-induced fluorescence-whole column imaging detection in which protein-DNA complexes are separated with spatial resolution while dissociations of the complexes are dynamically monitored using a CCD camera with temporal resolution. This method allows for the discrimination of different complexes and the measurement of the individual dissociation rate constants.
Liu
Zhen
Z
Department of Chemistry, Nanjing University, Nanjing 210093, China. zhenliu@nju.edu.cn
Drabovich
Andrei P
AP
Krylov
Sergey N
SN
Pawliszyn
Janusz
J
D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D019075:Electrophoresis, Capillary; D005453:Fluorescence; D007525:Isoelectric Focusing / Q000295:instrumentation / Q000379:methods*; D007834:Lasers; D011485:Protein Binding
D004268:DNA-Binding Proteins; D004247:DNA