title: Application of response surface methodology in medium optimization for pyruvic acid production of Torulopsis glabrata TP19 in batch fermentation.
abstract: Response surface methodology (RSM) was used to optimize the fermentation medium for enhancing pyruvic acid production by Torulopsis glabrata TP19. In the first step of optimization, with Plackett-Burman design, ammonium sulfate, glucose and nicotinic acid were found to be the important factors affecting pyruvic acid production significantly. In the second step, a 2(3) full factorial central composite design and RSM were applied to determine the optimal concentration of each significant variable. A second-order polynomial was determined by the multiple regression analysis of the experimental data. The optimum values for the critical components were obtained as follows: ammonium sulfate 0.7498 (10.75 g/L), glucose 0.9383 (109.38 g/L) and nicotinic acid 0.3633 (7.86 mg/L) with a predicted value of maximum pyruvic acid production of 42.2 g/L. Under the optimal conditions, the practical pyruvic acid production was 42.4 g/L. The determination coefficient (R(2)) was 0.9483, which ensures adequate credibility of the model. By scaling up fermentation from flask to jar fermentor, we obtained promising results.
authors.affiliation: Tianjin Key Lab of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China. zj96sk@163.com
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Nian-fa
authors.initials: NF
authors.lastname: Gao
mesh_terms: D000465:Algorithms; D019149:Bioreactors / Q000382:microbiology*; D041221:Candida glabrata / Q000254:growth & development* / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D003198:Computer Simulation; D008954:Models, Biological; D019289:Pyruvic Acid / Q000378:metabolism*; D011786:Quality Control
chemical_list: D019289:Pyruvic Acid