Green fluorescent protein expression in the symbiotic basidiomycete fungus Hebeloma cylindrosporum.
The symbiotic basidiomycete Hebeloma cylindrosporum is a model fungal species used to study ectomycorrhizal symbiosis at the molecular level. In order to have a vital marker, we developed a green fluorescent protein (GFP) reporter system efficiently expressed in H. cylindrosporum using the sgfp coding region bordered by two introns fused to the saprophytic basidiomycete Coprinopsis cinerea cgl1 promoter. Expression of this reporter system was tested under different environmental conditions in two transformants, and glucose was shown to repress gfp expression. Such a reporter system will be used in plant-fungus interaction to evaluate sugar supply by the plant to the compatible mycorrhizal symbiont and to compare the expression of various genes of interest in the free-living mycelia, in the symbiotic (mycorrhizas) and the reproductive (fruit bodies) structures formed by H. cylindrosporum.
Rekangalt
David
D
Université de Lyon, Université Lyon 1, Ecologie Microbienne (UMR CNRS 5557, USC INRA 1193), Villeurbanne, France.
Verner
Marie-Christine
MC
Kües
Ursula
U
Walser
Piers James
PJ
Marmeisse
Roland
R
Debaud
Jean-Claude
JC
Fraissinet-Tachet
Laurence
L
D000363:Agaricales / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D037161:Galectins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D017930:Genes, Reporter; D005947:Glucose / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D009172:Mycology / Q000379:methods; D038821:Mycorrhizae; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013559:Symbiosis; D014170:Transformation, Genetic
D037161:Galectins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D005947:Glucose