title: Application of quantum dots to multicolor flow cytometry.
abstract: The coupling of quantum dots (QDs) to antibodies heralds a new era of versatility in multicolor flow cytometry. This chapter introduces the properties of QDs (as relevant to flow cytometric applications), the advantages derived from these properties, and the procedure for conjugating antibodies to QDs. Finally, we discuss strategies for choosing the combinations of QDs and antibodies best suited for multicolor experiments.
authors.affiliation: Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Pratip K
authors.initials: PK
authors.lastname: Chattopadhyay
authors.forename: Joanne
authors.initials: J
authors.lastname: Yu
authors.forename: Mario
authors.initials: M
authors.lastname: Roederer
mesh_terms: D000906:Antibodies / Q000737:chemistry; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D045663:Quantum Dots; D015203:Reproducibility of Results
chemical_list: D000906:Antibodies; D005456:Fluorescent Dyes