title: [Analysis of different mutations in regulatory sequence of prolactin gene during the formation of 17 beta-estradiol-induced prolactinoma in eutopic and ectopic pituitary of rats].
abstract: OBJECTIVE
  To analyze different mutations in regulatory sequence of prolactin (PRL) gene during the formation of 17 beta-estradiol (E2 ) -induced prolactinoma in eutopic and ectopic pituitary of rats.
  
  
  METHODS
  Male Sprague-Dawley rats transplanted with an isologaus pituitary under renal capsule were treated with subcutaneous implantation of an empty or E2-laden silastic capsule. Reverse transcription-polymerase chain reaction was employed to evaluate the expression of PRL mRNA in pituitary glands, and DNA sequencing was used to analyze the mutation in regulatory sequence of PRL gene.
  
  
  RESULTS
  After treated with E2 for 120 days, both the eutopic and ectopic pituitaries were three times more heavier than those from control group (P < 0. 01) , and the body weight of rats was decreased to 42. 90% of the control group (P < 0 01 ). The PRL mRNA expressions in glands from the eutopic and ectopic pituitaries 120 days after treated with E2 were much more than those in untreated pituitary glands (P <0. 01). DNA sequencing showed seven mutations in the regulatory sequence of PRL gene in the eutopic pituitaries 120 days after treated with E2 , while the mutation in the ectopic pituitaries was decreased.
  
  
  CONCLUSIONS
  Prolactinomas can be induced by chronic treatment with E2 in both the eutopic and the ectopic pituitaries transplanted under renal capsule distant from the hypothalamus. Different mechanisms exist in the formation of eutopic and ectopic prolactinomas.
authors.affiliation: Department of Physiology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
authors.forename: Feng-zhu
authors.initials: FZ
authors.lastname: Zhang
authors.forename: Quan-hui
authors.initials: QH
authors.lastname: Yang
authors.forename: Yun-chun
authors.initials: YC
authors.lastname: Mao
authors.forename: Rong-kun
authors.initials: RK
authors.lastname: Xu
mesh_terms: D000818:Animals; D004958:Estradiol; D008297:Male; D009154:Mutation; D010902:Pituitary Gland / Q000637:transplantation; D010911:Pituitary Neoplasms / Q000139:chemically induced* / Q000235:genetics; D011388:Prolactin / Q000235:genetics*; D015175:Prolactinoma / Q000139:chemically induced* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014184:Transplantation, Homologous
chemical_list: D012333:RNA, Messenger; D004958:Estradiol; D011388:Prolactin