title: Sergeia podlipaevi gen. nov., sp. nov. (Trypanosomatidae, Kinetoplastida), a parasite of biting midges (Ceratopogonidae, Diptera).
abstract: Three strains of a trypanosomatid protozoan were isolated from the midguts of two naturally infected species of biting midges [Culicoides (Oecacta) festivipennis and Culicoides (Oecacta) truncorum] and characterized by light and electron microscopy and by molecular techniques. Morphological characteristics and sequences of the 18S rRNA, 5S rRNA, spliced leader RNA and glycosomal glyceraldehyde-3-phosphate dehydrogenase genes indicate that the studied flagellates represent a novel phylogenetic lineage within the Trypanosomatidae. Based on phylogenetic analyses, the novel endosymbiont-free, monoxenous trypanosomatid was classified as Sergeia podlipaevi gen. nov., sp. nov. Interestingly, it is closely related to another trypanosomatid species that parasitizes the sand fly Lutzomyia evansi, a blood-sucking dipteran from South America. The type strain of S. podlipaevi sp. nov., ICUL/CZ/2000/CER3, was obtained from Malpighian tubes. Of 2518 females of seven species of biting midges trapped in the Czech Republic, more than 1.5 % were infected by trypanosomatid parasites. An unrelated insect species, Culicoides (Monoculicoides) nubeculosus, was experimentally infected with S. podlipaevi, demonstrating that its host range extends to different subgenera of biting midges.
authors.affiliation: Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic.
authors.forename: Milena
authors.initials: M
authors.lastname: Svobodová
authors.affiliation: Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Zídková
authors.affiliation: Department of Zoology, Faculty of Science, Charles University, Prague, Czech Republic.
authors.forename: Ivan
authors.initials: I
authors.lastname: Čepička
authors.affiliation: Biology Centre, Institute of Parasitology, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Oborník
authors.affiliation: Faculty of Biology, University of South Bohemia, České Budějovice (Budweis), Czech Republic.|Biology Centre, Institute of Parasitology, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic.
authors.forename: Julius
authors.initials: J
authors.lastname: Lukeš
authors.affiliation: Biology Centre, Institute of Parasitology, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic.|Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Votýpka
mesh_terms: D000818:Animals; D002519:Ceratopogonidae / Q000166:cytology / Q000469:parasitology* / Q000648:ultrastructure; D018105:DNA, Kinetoplast / Q000032:analysis; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D041981:Gastrointestinal Tract / Q000469:parasitology; D020459:Genes, rRNA; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / Q000235:genetics; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010802:Phylogeny; D016053:RNA, Protozoan / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014351:Trypanosomatina / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D018105:DNA, Kinetoplast; D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D016053:RNA, Protozoan; D012337:RNA, Ribosomal, 18S; D012341:RNA, Ribosomal, 5S; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)