Activities of enzymes that hydrolyze adenine nucleotides in platelets from rats experimentally demyelinated with ethidium bromide and treated with interferon-beta.
The activities of the enzymes NTPDase (EC 3.6.1.5, apyrase, CD39) and 5'-nucleotidase (EC 3.1.3.5, CD73) were analyzed in platelets from rats submitted to demyelination by ethidium bromide (EB) and treated with interferon beta (IFN-beta). The following groups were studied: I - control (saline), II - (saline and IFN-beta), III - (EB) and IV - (EB and IFN-beta). After 7, 15 and 30 days, the animals (n=7) were sacrificed and the platelets were separated by the method of Lunkes et al. [Lunkes, G., Lunkes D., Morsch, V., Mazzanti, C., Morsch, A., Miron, V., Schetinger, M.R.C., 2004. NTPDase and 5'-nucleotidase in rats alloxan- induced diabetes. Diabetes Research and Clinical Practice 65, 1-6]. NTPDase activity for ATP and ADP substrates was significantly lower in groups II and III after seven days, when compared to control (p<0.001). At fifteen days, ATP hydrolysis was significantly lower in group III and IV and higher in group II (p<0.001), while there was an activation of ADP hydrolysis in group II (p<0.001), when compared with the control. 5'-nucleotidase activity was significantly higher in group IV (p<0.001) after seven days, and lower in the groups III and IV (p<0.001) after fifteen days in relation to the control. No significant differences were observed in NTPDase and 5'-nucleotidase activities after thirty days. In conclusion, our study demonstrated that the hydrolysis of adenine nucleotides is modified in platelets of rats demyelinated and treated with IFN-beta.
Spanevello
Roselia M
RM
Pós Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil.
Mazzanti
Cinthia M
CM
Maldonado
Paula A
PA
Zanin
Rafael
R
Morsch
André
A
Hannel
Laura
L
Mazzanti
Alexandre
A
Festugatto
Rafael
R
Graça
Dominguita
D
Schmatz
Roberta
R
Loro
Vânia Lúcia
VL
Schetinger
Maria Rosa C
MR
Morsch
Vera M
VM
D015720:5'-Nucleotidase / Q000378:metabolism*; D000227:Adenine Nucleotides / Q000378:metabolism*; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D001081:Apyrase / Q000378:metabolism*; D001792:Blood Platelets / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D003711:Demyelinating Diseases / Q000097:blood / Q000188:drug therapy / Q000201:enzymology; D004195:Disease Models, Animal; D004996:Ethidium; D006868:Hydrolysis; D016899:Interferon-beta / Q000627:therapeutic use*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D000227:Adenine Nucleotides; D015703:Antigens, CD; D016899:Interferon-beta; D015720:5'-Nucleotidase; D001081:Apyrase; C067869:CD39 antigen; D004996:Ethidium