Quantitative Determination of Seco-Iridoid Glucosides from the Fruits of Ligustrum vulgare by HPLC.
A HPLC method for separation and quantitative determination of secoiridoid glucosides in fruit extracts from LIGUSTRUM VULGARE L. is described. A system using a reversed phase column and an ethanol-water gradient elution, was suitable for separation of Ligstroside, Oleuropein, Nüzhenide and LF (3) 8. The secoiridoid glucosides were detected at 235 nm. The detection limits were within 2 and 10 microg/ml. Ripe fruits yield about 8.85% of secoiridoid glucosides (dried weight).
Willems
M
M
Institut für Pharmazeutische Biologie, Johann Wolfgang-Goethe-Universität Frankfurt/Main, D-6000 Frankfurt/Main, Bundesrepublik Deutschland.