[Detection of qualitative abnormalities of HDL by measurement of prebeta1-HDL concentration].
Prebeta1-HDL is a native lipid-poor HDL that promotes cholesterol efflux from cell membranes. Prebeta1-HDL is a good substrate of lecithin-cholesterol acyltransferase (LCAT) and is converted into a-migrating spherical HDL by LCAT activity. Prebeta1-HDL is probably secreted from the liver and is also generated from a-HDL by several regulatory factors. At present, prebeta1-HDL concentration is determined by native two-dimensional gel electrophoresis, ultrafiltration-isotope dilution method, or immunoassay using monoclonal antibody. Plasma samples for immunoassay are pretreated with 50% sucrose, which stabilizes prebeta1-HDL during storage at -20 degrees C as well as at 4 degrees C. We determined the LCAT-dependent conversion rate of prebeta1-HDL to detect abnormal HDL metabolism. In this review, we discuss the physiological role of prebeta1 HDL and the clinical significance of plasma prebeta1-HDL concentration.
Miida
Takashi
T
Division of Central Clinical Laboratory, Niigata University Medical and Dental Hospital, Niigata.
D000818:Animals; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D008076:Cholesterol, HDL / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D053442:High-Density Lipoproteins, Pre-beta / Q000097:blood* / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D007118:Immunoassay; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000502:physiology; D014462:Ultrafiltration
D008076:Cholesterol, HDL; D053442:High-Density Lipoproteins, Pre-beta; D007862:Phosphatidylcholine-Sterol O-Acyltransferase