title: The Instability of Neurospora Duplication Dp(IL-->IR)H4250 , and Its Genetic Control.
abstract: Previous work (Newmeyer and Taylor 1967) showed that a nontandem duplication, Dp(IL-->IR)H4250, is regularly produced by recombination in crosses heterozygous for the effectively terminal pericentric inversion In(IL-->IR)H4250. The duplications initially have strongly inhibited growth because they are heterozygous for mating type, which behaves like a vegetative-incompatibility (het) locus. Such cultures "escape" from the inhibition as a result of events that eliminate the mating-type heterozygosity. The product of a given escape event may be barren or fertile. (Neurospora duplications are characteristically barren; that is, when crossed, they make many perithecia but few ascospores.)-The present paper reports on a genetic analysis of the instability of Dp(IL-->IR)H4250 . Most of the barren escape products behave as if due either to mitotic crossovers, which make mating type and distal markers homozygous, or to very long deletions which uncover mating type and all distal markers; presumably the latter would retain enough duplicated material to render them barren. It is difficult to distinguish between these two possibilities, but homozygosis seems more probable and has been clearly demonstrated in one case. Only a few barren escapes could be due to short deletions or to changes at the mating-type locus.-The fertile escape products appear to be euploid. Most of these behave as if they arose by precise deletion of one or the other duplicated segment, thus restoring one of the parental sequences. A large majority of the precise deletions restore normal sequence; only a few restore inversion sequence. Preferential restoration of the normal sequence has also been found by other workers for Neurospora duplications from several other rearrangements. A hypothesis is presented to explain these findings; it is posulated that the precise deletions result from mitotic crossing over in homologous material located at chromosome tips and tip-break-points.-There is a smaller group of fertile escapes that are unlike either parental sequence; at least one of these involves a chromosome break outside the duplicated region.-Duplications in which the vegetative incompatibility is suppressed by the unlinked modifier tol are extremely barren; they only rarely lose a duplicated segment so as to become fertile.-The instability of Dp(IL-->IR)H4250 , with and without tol, is markedly altered by factors in the genetic background. The two factors studied in detail have qualitatively different effects.
authors.affiliation: Department of Biological Sciences, Stanford University, Stanford, California 94305.
authors.forename: D
authors.initials: D
authors.lastname: Newmeyer
authors.forename: D R
authors.initials: DR
authors.lastname: Galeazzi