Inhibition by epigallocatechin gallate of CoCl2-induced apoptosis in rat PC12 cells.
Epigallocatechin-3-gallate (EGCG) is a major constituent of green tea polyphenols. This study was aimed to investigate the possible mechanisms of EGCG-mediated inhibition against apoptosis in rat pheochromocytoma PC12 cells by exposure to CoCl(2). Exposure to CoCl(2) caused the generation of ROS and induced cell death with appearance of apoptotic morphology and DNA fragmentation. However, EGCG rescued the loss of viability in the cells exposed to CoCl(2) and led the reduction of DNA fragmentation and sub-G(1) fraction of cell cycle. Also, EGCG attenuated the CoCl(2)-induced disruption of mitochondrial membrane potential (DeltaPsim), release of cytochrome c from the mitochondria to cytosol and abolished the CoCl(2)-stimulated activities of the caspase cascades, caspase-9 and caspase-3. In addition, EGCG ameliorated the increase in the Bax to Bcl-2 ratio, a marker of apoptosis proceeding, induced by CoCl(2) treatment. Taken together, the present results suggest that EGCG inhibit the CoCl(2)-induced apoptosis of PC12 cells through the mitochondria-mediated apoptosis pathway involved in modulating the Bcl-2 family.
Jung
Ji-Yeon
JY
Dental Science Research Institute, School of Dentistry, 2nd Stage of Brain Korea 21 for School of Dentistry, Chonnam National University, Gwang Ju 500-757, South Korea.
Mo
Hyun-Chul
HC
Yang
Kyu-Ho
KH
Jeong
Yeon-Jin
YJ
Yoo
Hyeon-Gyeong
HG
Choi
Nam-Ki
NK
Oh
Won-Mann
WM
Oh
Hee-Kyun
HK
Kim
Sun-Hun
SH
Lee
Jin-Ha
JH
Kim
Hyun-Jin
HJ
Kim
Won-Jae
WJ
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D003035:Cobalt / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D053938:DNA Fragmentation / Q000187:drug effects; D004587:Electrophoresis, Agar Gel; D005434:Flow Cytometry; D019254:Genes, bcl-2 / Q000235:genetics; D020925:Hypoxia-Ischemia, Brain / Q000473:pathology; D008564:Membrane Potentials / Q000187:drug effects; D051336:Mitochondrial Membranes / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016716:PC12 Cells; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D013778:Tetrazolium Salts; D013844:Thiazoles
D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; D013778:Tetrazolium Salts; D013844:Thiazoles; D003035:Cobalt; D002392:Catechin; C045651:epigallocatechin gallate; D053148:Caspase 3; D053453:Caspase 9; C022616:thiazolyl blue; C018021:cobaltous chloride