title: Characterization of the SOS response of Pseudomonas fluorescens strain DC206 using whole-genome transcript analysis.
abstract: DNA microarray technology was used to survey changes in gene expression in Pseudomonas fluorescens after mitomycin C treatment. As expected, genes associated with the SOS response were upregulated, such as those encoding the recombination protein RecA, DNA repair protein RecN, excinuclease ABC subunit A UvrA, and the LexA repressor protein. Interestingly, expression of 33 clustered bacteriophage-like genes was upregulated, suggesting that mitomycin C (MMC) may induce a prophage resident in the P. fluorescens genome. However, no phage particles were detected in P. fluorescens strain DC206 that had been treated with MMC using transmission electron microscopy. The same preparation failed to produce phage plaques on lawns of any of 10 different Pseudomonas strains tested, indicating that the 33 bacteriophage-like gene cluster represents a defective prophage.
authors.affiliation: The Dow Chemical Company, Oberlin Drive, San Diego, CA 92121, USA. hjin@dow.com
authors.forename: Hongfan
authors.initials: H
authors.lastname: Jin
authors.forename: Diane M
authors.initials: DM
authors.lastname: Retallack
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Stelman
authors.forename: C Douglas
authors.initials: CD
authors.lastname: Hershberger
authors.forename: Tom
authors.initials: T
authors.lastname: Ramseier
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial; D016685:Mitomycin / Q000494:pharmacology*; D020411:Oligonucleotide Array Sequence Analysis; D017105:Pseudomonas Phages / Q000187:drug effects / Q000502:physiology; D011551:Pseudomonas fluorescens / Q000187:drug effects / Q000235:genetics / Q000502:physiology / Q000821:virology; D013014:SOS Response, Genetics; D014158:Transcription, Genetic; D015854:Up-Regulation; D014775:Virus Activation / Q000187:drug effects / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D016685:Mitomycin