Effect of carbon dioxide pneumoretroperitoneum on bacterial translocation in an experimental retroperitoneoscopy model.
OBJECTIVE
To evaluate the effect of artificial CO2 pneumoretroperitoneum on bacterial translocation in an experimental retroperitoneoscopy model.


METHODS
Eighteen adult male New Zealand White rabbits weighing 2.5 to 3 kg were divided into two groups. Group 1 (control group) consisted of 6 rabbits, while the remaining 12 served as the pneumoretroperitoneum group (group 2). In group 1, the left retroperitoneal space was dissected with a 50-mL balloon without CO2 insufflation, and the animals were kept under anesthesia for 3 hours with the balloons inflated. In group 2, after balloon dissection as in group 1, CO2 insufflation was applied at 1 L/min to achieve a pressure of 10 to 12 mm Hg for 3 hours. Afterward, all animals were sacrificed, and samples were taken from the blood, retroperitoneal area, lungs, liver, mesentery, heart, kidneys, ureters, bladder, colon, small intestine, and spleen and carried to the microbiology laboratory in Carry-Blair medium. Bacterial growth was evaluated using standard techniques.


RESULTS
All animals survived the experimental procedures. None of the rabbits in the control group demonstrated any bacterial translocation in the sampled tissues. In the pneumoretroperitoneum group, one rabbit was found to have 10(2) colony-forming units of E. coli in the kidney, but this was considered to be the result of contamination, not translocation.


CONCLUSIONS
Carbon dioxide pneumoretroperitoneum does not seem to cause bacteremia or bacterial translocation in this experimental model. Retroperitoneoscopy probably does not create any additional risk of septic complications.
Tüzel
Emre
E
Department of Urology, Afyon Kocatepe University School of Medicine, Afyonkarahisar, Turkey. emretuzel@aku.edu.tr
Güler
Cem
C
Aktepe
Orhan Cem
OC
Saritaş
Kadir
K
Samli
M Murat
MM
Demirbaş
Murat
M
D000818:Animals; D018988:Bacterial Translocation / Q000187:drug effects*; D002245:Carbon Dioxide / Q000494:pharmacology*; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000502:physiology*; D008297:Male; D011817:Rabbits; D012188:Retropneumoperitoneum / Q000139:chemically induced* / Q000382:microbiology*
D002245:Carbon Dioxide