HPLC Analysis of the Flavonoid Glycosides from Betulae folium.
The main flavonoid glycosides from BETULAE folium are separated with a RP-HPLC method. The mobile phase for the separation of the seven most important compounds (rutin, hyperin, quercetin-3-arabinofuranoside, quercetin-3-arabinopyranoside, quercetin-3-rhamnoside, quercetin-3-glucuronide, and myricetin-3-galactoside) was optimized with the "PRISMA" model, which is a multisolvent optimization system. The isocratic separations were finished within 30 minutes and the peak purity was controlled with diode-array detection. The method can be applied for quantitative determination, as was demonstrated for different samples of B. PENDULA and B. PUBESCENS.
Dallenbach-Tölke
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Pharmazeutisches Institut, Eidgenössische Technische Hochschule Zürich, CH-8092 Zürich, Schweiz.
Nyirediy
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Meier
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B
Sticher
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O