Trehalose-6-phosphate Phosphatase from Mycobacterium tuberculosis induces humoral and cellular immune responses.
Trehalose-6-phosphate phosphatase is an enzyme strictly essential for the growth of mycobacteria. Subcellular fractionation of Mycobacterium tuberculosis and M. bovis bacillus Calmette-Guérin (BCG) located the trehalose-6-phosphate phosphatase in the cell wall and membrane fractions. Trehalose-6-phosphate phosphatase induced an increased Th1-type immune response in mice, characterized by an elevated level of interferon-gamma in antigen-stimulated splenocyte culture and a strong IgG2a antibody response. The trehalose-6-phosphate phosphatase was recognized by the sera of tuberculosis patients and BCG-vaccinated donors. The mycobacterial trehalose-6-phosphate phosphatase is an immunodominant antigen, and it may be a candidate for vaccine development for the control of tuberculosis.
Zhang
Min
M
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai, China.
Yang
Yanping
Y
Xu
Ying
Y
Qie
Yaqing
Y
Wang
Jiuling
J
Zhu
Bingdong
B
Wang
Qingzhong
Q
Jin
Ruiliang
R
Xu
Shengfeng
S
Wang
Honghai
H
D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D001500:BCG Vaccine / Q000276:immunology / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D051379:Mice; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis / Q000201:enzymology* / Q000276:immunology; D010744:Phosphoric Monoester Hydrolases / Q000276:immunology* / Q000378:metabolism / Q000494:pharmacology; D014376:Tuberculosis / Q000276:immunology* / Q000382:microbiology
D001500:BCG Vaccine; D007074:Immunoglobulin G; C023178:trehalose-phosphatase; D010744:Phosphoric Monoester Hydrolases