title: In vivo thickness and birefringence determination of the human retinal nerve fiber layer using polarization-sensitive optical coherence tomography.
abstract: Thinning of the retinal nerve fiber layer and changes in retinal nerve fiber layer birefringence may both precede clinically detectable glaucomatous vision loss. We present in vivo thickness and depth-resolved birefringence measurements of the human retinal nerve fiber layer (RNFL) by use of polarization-sensitive optical coherence tomography (PS-OCT). Using a fiber-based PS-OCT setup real-time images of the human retina in vivo were recorded, co-registered with retinal video images of the location of PS-OCT scans. PS-OCT scans around the optic nerve head (ONH) of two healthy young volunteers were made using 10 concentric circles of increasing radius. Both the mean retinal nerve fiber layer thickness and mean retinal nerve fiber birefringence for each of 48 sectors on a circle were determined. The retinal nerve fiber layer thickness and birefringence varied as a function of sector around the ONH. Measured double pass phase retardation per unit depth values around the ONH range between 0.10 and 0.35 degrees/microm. The retinal nerve fiber layer becomes thinner with increasing distance from the ONH. In contrast, the birefringence does not vary significantly with increasing distance from the ONH.
authors.affiliation: Harvard Medical School, Wellman Center for Photomedicine, MGH, Boston, MA 02114, USA.
authors.forename: B
authors.initials: B
authors.lastname: Cense
authors.forename: T C
authors.initials: TC
authors.lastname: Chen
authors.forename: J F
authors.initials: JF
authors.lastname: de Boer
mesh_terms: D000886:Anthropometry; D001718:Birefringence; D004867:Equipment Design; D006801:Humans; D009412:Nerve Fibers; D009898:Optic Disk / Q000033:anatomy & histology; D010786:Photoreceptor Cells / Q000033:anatomy & histology / Q000166:cytology*; D012016:Reference Values; D012160:Retina / Q000033:anatomy & histology*; D012165:Retinal Ganglion Cells / Q000145:classification / Q000166:cytology; D041623:Tomography, Optical Coherence / Q000295:instrumentation* / Q000379:methods*