title: Carbenoxolone and triterpenoids inhibited mucin secretion from airway epithelial cells.
abstract: This study investigated whether carbenoxolone, oleanolic acid and ursolic acid affect ATP-induced mucin secretion from cultured airway epithelial cells. Confluent primary hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled using (3)H-glucosamine for 24 h and chased for 30 min in the presence of varying concentrations of each agent to assess the effects on (3)H-mucin secretion. The possible cytotoxicity of each agent was investigated with a lactate dehydrogenase assay. The results were as follows: (1) carbenoxolone, oleanolic acid and ursolic acid significantly inhibited the secretion of airway mucin induced by ATP; (2) none of the compounds showed significant cytotoxicity at any concentration. This result suggests that carbenoxolone, oleanolic acid and ursolic acid can regulate 'mucin secretion induced by ATP'--phenomenon simulating mucus overproduction from inflamed airway epithelial cells--y directly acting on airway mucin-secreting cells.
authors.affiliation: Department of Pharmacology, College of Medicine, Chungnam National University, Daejeon, Korea.
authors.forename: Ho Jin
authors.initials: HJ
authors.lastname: Heo
authors.forename: Cheolsu
authors.initials: C
authors.lastname: Kim
authors.forename: Hyun Jae
authors.initials: HJ
authors.lastname: Lee
authors.forename: Young Sik
authors.initials: YS
authors.lastname: Kim
authors.forename: Sam Sik
authors.initials: SS
authors.lastname: Kang
authors.forename: Un Kyo
authors.initials: UK
authors.lastname: Seo
authors.forename: Yun Hee
authors.initials: YH
authors.lastname: Kim
authors.forename: Yang Chun
authors.initials: YC
authors.lastname: Park
authors.forename: Jeong Ho
authors.initials: JH
authors.lastname: Seok
authors.forename: Choong Jae
authors.initials: CJ
authors.lastname: Lee
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism / Q000502:physiology; D000818:Animals; D002229:Carbenoxolone / Q000494:pharmacology*; D002478:Cells, Cultured; D006224:Cricetinae; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D009077:Mucins / Q000378:metabolism*; D009828:Oleanolic Acid / Q000494:pharmacology*; D020545:Respiratory Mucosa / Q000187:drug effects* / Q000378:metabolism; D014315:Triterpenes / Q000494:pharmacology*; D000097245:Ursolic Acid
chemical_list: D000255:Adenosine Triphosphate; D002229:Carbenoxolone; D009077:Mucins; D009828:Oleanolic Acid; D014315:Triterpenes; D000097245:Ursolic Acid