Identification of a necessary element for Toxoplasma gondii SAG1 gene expression.
SAG1 codes for the stage-specific major surface antigen P30 of Toxoplasma gondii (T. gondii) tachyzoites. Six tandemly repeated, conserved 27 bp cassettes in the region from -231 to -70 bp were previously confirmed to be essential for high-level expression of SAG1 and serve as a positioning element directing the initiation of transcription. We demonstrate here that an element located between +19 and +28 bp is necessary for SAG1 gene expression by using deletion mutagenesis analysis and electrophoresis mobility shift assay (EMSA). This will provide an insight into the regulatory mechanisms of SAG1 gene expression.
Zhang
Jiaqin
J
Department of Parasitology, School of Medicine, Shandong University, and Nephrology Department, The Second Hospital of Shandong University, PR China.
Gu
Qinmin
Q
Hou
Xianghua
X
Zhou
Huaiyu
H
Cong
Hua
H
Li
Ying
Y
Zhao
Qunli
Q
Li
Shouqing
S
D000818:Animals; D000953:Antigens, Protozoan / Q000096:biosynthesis / Q000235:genetics*; D000954:Antigens, Surface / Q000096:biosynthesis / Q000235:genetics; D015342:DNA Probes / Q000737:chemistry; D024202:Electrophoretic Mobility Shift Assay; D005786:Gene Expression Regulation / Q000502:physiology*; D005822:Genetic Vectors; D006801:Humans; D008156:Luciferases / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D011401:Promoter Regions, Genetic / Q000235:genetics; D015800:Protozoan Proteins / Q000096:biosynthesis / Q000235:genetics*; D016053:RNA, Protozoan / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020080:Tandem Repeat Sequences / Q000502:physiology; D014122:Toxoplasma / Q000235:genetics* / Q000276:immunology; D014162:Transfection
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D015342:DNA Probes; D015800:Protozoan Proteins; D016053:RNA, Protozoan; C047168:SAG1 antigen, Toxoplasma; D008156:Luciferases