title: Inhibitory effects of methyl brevifolincarboxylate isolated from Phyllanthus niruri L. on platelet aggregation.
abstract: A platelet-aggregatory inhibitor was isolated from the 50% MeOH extract of Phyllanthus niruri L. leaf. Its structure was determined to be methyl brevifolincarboxylate on the basis of the 1H-, 13C-NMR, and high-resolution mass spectral data. We compared the antiplatelet aggregatory effects of the constituent with adenosine, a well-known inhibitor of platelet aggregation. Platelet aggregation was induced by collagen or adenosine 5'-diphosphate as an activating agent; the extent of inhibition was monitored with a platelet aggregometer employing a laser-scattering method. The inhibitory effects of methyl brevifolincarboxylate were found to be as potent as adenosine that is known to act on an A2A subtype receptor.
authors.affiliation: Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo, Japan. iizuka@hoshi.ac.jp
authors.forename: Toru
authors.initials: T
authors.lastname: Iizuka
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nagai
authors.forename: Akemi
authors.initials: A
authors.lastname: Taniguchi
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Moriyama
authors.forename: Keiko
authors.initials: K
authors.lastname: Hoshi
mesh_terms: D001578:Benzopyrans / Q000302:isolation & purification / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects; D006801:Humans; D029803:Phyllanthus / Q000737:chemistry*; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D001578:Benzopyrans; D010975:Platelet Aggregation Inhibitors; C087786:methyl brevifolincarboxylate