Biosynthesis of intracellular 5-aminolevulinic acid by a newly identified halotolerant Rhodobacter sphaeroides.
Of 23 strains of halotolerant (up to 12% w/v NaCl) photosynthetic bacteria isolated from various sources, one isolate, SH5, accumulated intracellular 5-aminolevulinic acid (ALA) at 0.45 microg/g dry cell wt (DCW) growing aerobically in the dark. The strain was identified as Rhodobacter sphaeroides using 16S rDNA sequencing. Biosynthesis of ALA was enhanced to 14 microg/g DCW using modified glutamate/glucose (50 mM) medium with the addition of 10 mM levulinic acid after 24 h cultivation. Addition of 30 microM Fe(2+) to this medium increased the yield to 226 microg/g DCW.
Tangprasittipap
Amornrat
A
Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand.
Prasertsan
Poonsuk
P
Choorit
Wanna
W
Sasaki
Ken
K
D000622:Aminolevulinic Acid / Q000378:metabolism*; D001483:Base Sequence; D003470:Culture Media; D004275:DNA, Ribosomal / Q000235:genetics; D005798:Genes, Bacterial; D007501:Iron / Q000494:pharmacology; D007982:Levulinic Acids / Q000494:pharmacology; D008969:Molecular Sequence Data; D010788:Photosynthesis / Q000187:drug effects; D010802:Phylogeny; D012242:Rhodobacter sphaeroides / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D013997:Time Factors
D003470:Culture Media; D004275:DNA, Ribosomal; D007982:Levulinic Acids; D000622:Aminolevulinic Acid; D007501:Iron; C032246:levulinic acid