Correlates of serum matrix metalloproteinase-8 (MMP-8) concentrations in nondiabetic subjects without cardiovascular disease.
BACKGROUND
Matrix metalloproteinase-8 (MMP-8) has been implicated in the pathogenesis of cardiovascular disease. We evaluated the relative contribution of genetic and non-genetic variables to serum MMP-8 concentrations in nondiabetic subjects without known cardiovascular disease.


METHODS
A blood sample was obtained from 100 subjects > or =18 y. Total serum MMP-8 concentrations were assayed by ELISA. MMP8 genotypes (-799 C/T and -381 A/G) were determined by PCR-pyrosequencing.


RESULTS
MMP-8 concentrations were significantly higher in subjects with the metabolic syndrome (n=40) compared to those without the metabolic syndrome (11.71 vs 6.81 ng/ml, p<0.001) and in current smokers compared to non-smokers (13.51 vs 7.53 ng/ml, p=0.001). MMP-8 concentrations were significantly correlated with body mass index, triglyceride/HDL ratio, triglycerides, fasting plasma glucose, and age. MMP-8 concentrations were not significantly different between MMP8 genotype groups. In regression analysis, variables that were significantly associated with serum MMP-8 concentrations were presence of the metabolic syndrome and smoking (p<0.001 and p=0.009, respectively), accounting for 21.4% of the variability in serum MMP-8 concentrations.


CONCLUSIONS
Our data demonstrate that the metabolic syndrome and smoking are independently associated with elevated serum MMP-8 concentrations. The relationship between MMP-8 concentrations and cardiovascular risk merits further investigation.
Aquilante
Christina L
CL
Department of Pharmaceutical Sciences, University of Colorado at Denver and Health Sciences Center, School of Pharmacy, Denver, Colorado, USA. Christina.aquilante@UCHSC.edu
Beitelshees
Amber L
AL
Zineh
Issam
I
D000328:Adult; D002318:Cardiovascular Diseases / Q000175:diagnosis; D003920:Diabetes Mellitus / Q000175:diagnosis; D005260:Female; D006801:Humans; D008297:Male; D020784:Matrix Metalloproteinase 8 / Q000097:blood* / Q000235:genetics; D024821:Metabolic Syndrome / Q000175:diagnosis*; D011110:Polymorphism, Genetic; D012907:Smoking / Q000097:blood*
D020784:Matrix Metalloproteinase 8