title: [The N-terminal pro-brain natriuretic peptide (NT-proBNP) assay with the Stratus CS analyzer].
abstract: BACKGROUND
  NT-proBNP is an efficient biomarker for the evaluation, management and prognosis of patients with heart failure.
  
  
  METHODS
  We evaluated the analytical performance of the NT-proBNP immunoenzymatic assay with the Stratus CS semi-automated analyzer in two hospital laboratories. The characteristics assessed included imprecision, functional sensitivity, linearity/recovery, interferences study, high-dose hook effect and a comparison of Acute Care(TM) pPBNP (on Stratus)CS) versus PBNP (on Dimension HM) results on patient heparinized plasma samples.
  
  
  RESULTS
  Total imprecision reached < 5% coefficient of variation at NT-proBNP concentrations of 186-19,649 ng/L; recovery values for diluted samples were between 89.0 and 110.0 %; functional sensitivity reached 21 ng/L; there was no high-dose hook effect at concentrations up to 400,000 ng/L; hemaoglobin affected negatively but <10% the NT-proBNP assay, while bilirubin and triglycerides did not affected it more than 5%; Stratus CS results were strongly correlated with Dimension HM results (R(2)=1,00).
  
  
  CONCLUSIONS
  The Stratus CS Acute Care pPBNP assay demonstrated excellent analytical performance which agreed with the Dimension HM PBNP assay. This analyzer is therefore suitable for use by low NT-proBNP test volume laboratories, and also by Emergency departments and Intensive care units.
authors.affiliation: Laboratoire de Biochimie A, Hôpital Cochin, Assistance Publique - Hôpitaux de Paris (AP-HP), Paris. camille.gobeaux@cch.ap-hop-paris.fr
authors.forename: C
authors.initials: C
authors.lastname: Chenevier-Gobeaux
authors.forename: D
authors.initials: D
authors.lastname: Guillot
authors.forename: J
authors.initials: J
authors.lastname: Ursulet
authors.forename: J-L
authors.initials: JL
authors.lastname: Paul
authors.forename: O G
authors.initials: OG
authors.lastname: Ekindjian
authors.forename: D
authors.initials: D
authors.lastname: Desmoulins
authors.forename: M-O
authors.initials: MO
authors.lastname: Benoit
mesh_terms: D015415:Biomarkers / Q000097:blood; D006333:Heart Failure / Q000097:blood*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D016014:Linear Models; D020097:Natriuretic Peptide, Brain / Q000097:blood*; D010446:Peptide Fragments / Q000097:blood*; D015203:Reproducibility of Results
chemical_list: D015415:Biomarkers; D010446:Peptide Fragments; C109794:pro-brain natriuretic peptide (1-76); D020097:Natriuretic Peptide, Brain