Synthesis of oligo(ethylenediamino)-beta-cyclodextrin modified gold nanoparticle as a DNA concentrator.
A novel oligo(ethylenediamino)-beta-cyclodextrin-modified gold nanoparticle (OEA-CD-NP) was synthesized as a vector for DNA binding and comprehensively investigated by means of absorption and circular dichroism spectroscopies as well as transmission electron microscopy, and its plasmid transfection efficiency as a carrier into cultivated cells in vitro was also evaluated. Possessing many hydrophobic cavities at the outer space, OEA-CD-NP may have a capability of carrying biological and/or medicinal substrates into cells, which will make it potentially applicable in many fields of material science and biological technology. In contrast with OEA-CD-NP, the oligo(ethylenediamino)-lipoic amido-modified gold nanoparticle (OEA-L-NP) without CD was synthesized to investigate the interaction with DNA. The results showed that OEA-L-NPs could only weakly bind DNA.
Wang
Hao
H
Department of Chemistry, State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin, PR China.
Chen
Yong
Y
Li
Xiao-Yun
XY
Liu
Yu
Y
D045744:Cell Line, Tumor; D002942:Circular Dichroism; D003505:Cyclodextrins / Q000138:chemical synthesis*; D004247:DNA / Q000737:chemistry*; D005029:Ethylenediamines; D006046:Gold / Q000737:chemistry*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D017348:Microscopy, Electron, Scanning Transmission; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D014162:Transfection
D003505:Cyclodextrins; D005029:Ethylenediamines; D006046:Gold; D004247:DNA