The pattern of viral persistence in monkeys intra-tracheally infected with Simian varicella virus.
In situ PCR (ISPCR) and in situ hybridisation (ISH) was performed on 32 tissues from 10 monkeys, intra-tracheally (IT) infected with simian varicella virus (SVV) and 5 tissues from 3 uninfected control animals. The results showed persistence of SVV DNA up to 2 years post-infection (pi) and the localisation of SVV to be confined to neurons except at time points 9 and 10 months pi where SVV positive satellite cells were also detected. There was no evidence for transcription of SVV ORFs 63 and 21 in the ganglia of the one IT infected and 2 naturally infected monkeys investigated using RNA ISH.
Grinfeld
Esther
E
Department of Neurology, Institute of Neurological Sciences, University of Glasgow, Southern General Hospital, 1345 Govan Road, Glasgow G51 4TF, Scotland, UK.
Kennedy
Peter G E
PG
D000818:Animals; D002522:Chlorocebus aethiops; D004195:Disease Models, Animal; D006566:Herpesviridae Infections / Q000821:virology*; D007442:Intubation, Intratracheal; D051379:Mice; D051381:Rats; D018140:Varicellovirus / Q000235:genetics / Q000502:physiology*