title: Applications of high-throughput RNA interference screens to problems in cell and developmental biology.
abstract: RNA interference (RNAi) in tissue culture cells has emerged as an excellent methodology for identifying gene functions systematically and in an unbiased manner. Here, we describe how RNAi high-throughput screening (HTS) in Drosophila cells are currently being performed and emphasize the strengths and weaknesses of the approach. Further, to demonstrate the versatility of the technology, we provide examples of the various applications of the method to problems in signal transduction and cell and developmental biology. Finally, we discuss emerging technological advances that will extend RNAi-based screening methods.
authors.affiliation: Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02175, USA. perrimon@receptor.med.harvard.edu
authors.forename: Norbert
authors.initials: N
authors.lastname: Perrimon
authors.forename: Bernard
authors.initials: B
authors.lastname: Mathey-Prevot
mesh_terms: D000818:Animals; D015509:Developmental Biology; D016678:Genome; D006801:Humans; D010641:Phenotype; D034622:RNA Interference