title: Residues around the catalytic pocket of DdsA are important for isoprenoids chain elongation.
abstract: Nineteen mutants of decaprenyl diphosphate synthase from Gluconobacter suboxydans were generated and their production of ubiquinones (UQs) was compared to that of the wild type protein. The accessible surface area and the polarity of amino acid around the catalytic pocket wield remarkable influences on the isoprenoid chain elongation of UQs.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, Mailbox 431, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Liu
authors.forename: Qinsheng
authors.initials: Q
authors.lastname: Yuan
authors.forename: Huizhan
authors.initials: H
authors.lastname: Zhang
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D000596:Amino Acids / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D009154:Mutation / Q000235:genetics; D017434:Protein Structure, Tertiary; D013379:Substrate Specificity; D013729:Terpenes / Q000378:metabolism*; D014451:Ubiquinone / Q000096:biosynthesis*
chemical_list: D000596:Amino Acids; D013729:Terpenes; D014451:Ubiquinone; D019883:Alkyl and Aryl Transferases; C039821:decaprenyl pyrophosphate synthetase