Use of controlled ovulation of the dominant follicle to assess oocyte maturation during natural menstrual cycles in rhesus macaques.
To determine the practicality of controlled ovulation of the dominant follicle as a technique to study meiotic maturation of oocytes during contraceptive research, we developed a technique for aspiration of the single dominant follicle using a dual-needle continuous irrigation technique 27 hours after an ovulatory stimulus. All of the oocytes (3/3) recovered from control animals, but only 1/6 (17%) of oocytes from animals treated with the meiotic inhibitor ORG 9935 exhibited germinal vesicle breakdown, indicating resumption of meiosis.
Jensen
Jeffrey T
JT
Department of Obstetrics & Gynecology, Oregon Health & Science University, Portland, Oregon 97239, USA. jensenje@ohsu.edu
Stanley
Jessica E
JE
Zelinski
Mary B
MB
Stouffer
Richard L
RL
Jacob
Darla
D
Fanton
John
J
D000818:Animals; D005260:Female; D008253:Macaca mulatta; D008597:Menstrual Cycle / Q000502:physiology*; D009865:Oocytes / Q000502:physiology*; D006080:Ovarian Follicle / Q000502:physiology*; D010062:Ovulation Induction; D012016:Reference Values