Exiguobacterium profundum sp. nov., a moderately thermophilic, lactic acid-producing bacterium isolated from a deep-sea hydrothermal vent.
A facultatively anaerobic, halotolerant, moderately thermophilic and non-sporulating bacterium, designated strain 10C(T), was isolated from deep-sea hydrothermal vent samples collected on the 13 degrees N East Pacific Rise at a depth of approximately 2600 m. Cells of strain 10C(T) were Gram-positive, motile rods, and grew optimally at 45 degrees C (range 12-49 degrees C), pH 7.0 (range pH 5.5-9.5) and 0-2 % NaCl (range 0-11 %). (+)-L-Lactate was the main organic acid detected from carbohydrate fermentation with traces of formate, acetate and ethanol. Strain 10C(T) was catalase-positive, oxidase-negative and reduced nitrate to nitrite under anaerobic conditions. The DNA G+C content was 50.4 mol%. Its closest phylogenetic relatives were Exiguobacterium aestuarii TF-16(T) and Exiguobacterium marinum TF-80(T) (16S rRNA gene sequence similarity >99 %). However, strain 10C(T) differed genotypically from these two Exiguobacterium species as indicated by DNA-DNA relatedness data. Therefore, on the basis of phenotypic, genotypic and phylogenetic characteristics, strain 10C(T) is considered to represent a novel species of the genus Exiguobacterium, for which the name Exiguobacterium profundum sp. nov. is proposed. The type strain is 10C(T) (=CCUG 50949(T)=DSM 17289(T)).
Crapart
Sylvaine
S
IRD, UMR180, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
Fardeau
Marie-Laure
ML
IRD, UMR180, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
Cayol
Jean-Luc
JL
IRD, UMR180, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
Thomas
Pierre
P
IRD, UMR180, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
Sery
Christian
C
IRD, UMR180, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
Ollivier
Bernard
B
IRD, UMR180, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
Combet-Blanc
Yannick
Y
IRD, UMR180, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
D000693:Anaerobiosis; D001406:Bacillaceae / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D050260:Carbohydrate Metabolism; D002374:Catalase / Q000032:analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry; D020459:Genes, rRNA; D045482:Hot Springs / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000378:metabolism; D008969:Molecular Sequence Data; D009566:Nitrates / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000032:analysis; D010140:Pacific Ocean; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D012965:Sodium Chloride; D013171:Spores, Bacterial; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D009566:Nitrates; D009573:Nitrites; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D019344:Lactic Acid; D012965:Sodium Chloride; D010088:Oxidoreductases; D002374:Catalase