Isolation and characterization of a mannoglucan from edible Cordyceps sinensis mycelium.
Cordyceps sinensis is a well known tonic food or invigorant with broad-spectrum medicinal properties that is widely used in the People's Republic of China. A neutral mannoglucan 1 with a molar mass of approximately 7.7x10(3) Da was obtained from the 0.05 M acetate buffer extract of C. sinensis mycelium. It had [alpha](D)(20)+126 (c 0.2, H(2)O) and consisted of Man and Glc units in the molar ratio of 1:9. A combination of chemical analysis and NMR and IR spectroscopy together with digestion with alpha-D-amylase showed 1 to have a alpha-D-glucan backbone with (1-->4)- and (1-->3)-linkages, and the side chains of alpha-D-(1-->6)-Manp were attached to the backbone via O-6 of alpha-(1-->3)-Glcp residues. Compound 1 showed weak cytotoxicity activity against SPC-I (IC(50)=63 microg/mL) cancer line, and no obvious cytotoxicity activities against BCAP37 (IC(50)>100 microg/mL) and SW480 (IC(50)>100 microg/mL) cancer lines.
Wu
Yalin
Y
Department of Chemistry, Zhejiang University, Hangzhou 310027, People's Republic of China.
Hu
Nan
N
Pan
Yuanjiang
Y
Zhou
Lijun
L
Zhou
Xuxia
X
D002240:Carbohydrate Sequence; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002850:Chromatography, Gel; D032661:Cordyceps / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D008970:Molecular Weight; D025282:Mycelium / Q000737:chemistry*; D010084:Oxidation-Reduction; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000091402:Prohibitins; D013055:Spectrophotometry, Infrared; D000516:alpha-Amylases / Q000737:chemistry / Q000378:metabolism
C000717331:PHB2 protein, human; D011134:Polysaccharides; D000091402:Prohibitins; C493955:mannoglucan; D000516:alpha-Amylases