Red wine extract prevents neuronal apoptosis in vitro and reduces mortality of transgenic mice.
In this work, we have investigated the effects of nutritional antioxidants as antidegenerative agents on glutamate-induced apoptosis in primary cultures of cerebellar granule neurons (CGNs). Glutamate-induced apoptosis is also associated with intracellular [Ca(2+)]i overload, generation of reactive oxygen species (ROS), depression of cell energy metabolism, cytochrome c release, and increase in caspase-3 activity. Pretreatment (3 h) with red wine extract (5 microg/mL) and ascorbic acid (30 microM) blocks glutamate-induced apoptosis in CGNs. In vivo experiments carried out on transgenic mice expressing the human mutated Cu, Zn superoxide dismutase (SOD1) G93A (mSOD1(G93A)) show that mice fed with lyophilized red wine have significantly increased survival as compared to control, untreated animals.
Amodio
Rosalba
R
Experimental Oncology Unit--Department of Oncology, M. Ascoli, ARNAS-Civico, Palermo, Italy.
Esposito
Ennio
E
De Ruvo
Caterina
C
Bellavia
Vincenzo
V
Amodio
Emanuele
E
Carruba
Giuseppe
G
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002118:Calcium; D053148:Caspase 3 / Q000378:metabolism; D002478:Cells, Cultured; D045304:Cytochromes c / Q000378:metabolism; D018698:Glutamic Acid / Q000633:toxicity; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D009474:Neurons / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics; D000072105:Superoxide Dismutase-1; D016019:Survival Analysis; D014920:Wine
D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; C000606290:SOD1 protein, human; D018698:Glutamic Acid; D045304:Cytochromes c; C000606291:Sod1 protein, mouse; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D053148:Caspase 3; D002118:Calcium