title: Interaction of adrenomedullin and calcitonin gene-related peptide with the periodontal pathogen Porphyromonas gingivalis.
abstract: The nature of the interaction between Porphyromonas gingivalis and the multifunctional peptides adrenomedullin and calcitonin gene-related peptide (CGRP) was investigated. Growth of P. gingivalis was not inhibited in the presence of either of these peptides [minimal inhibitory concentration (MIC)>250 microg mL(-1)]. The ability of the arginine- and lysine-specific proteases from P. gingivalis to breakdown these peptides was investigated. Adrenomedullin and CGRP were incubated with culture supernatants from wild-type and protease gene knockout strains. No significant effect on antimicrobial activity against the indicator organism Escherichia coli BUE55 was found (MIC=6.25 microg mL(-1) in all cases). The role of anionic components on the surface of P. gingivalis, which may alter binding of these cationic peptides, was also investigated in relation to adrenomedullin. Growth of gene knockout strains lacking surface polysaccharide and capsule components was not inhibited (MIC>250 microg mL(-1)). It is suggested that a lack of sensitivity to adrenomedullin and CGRP may enable P. gingivalis to persist in the oral cavity and cause disease.
authors.affiliation: Oral Microbiology, Institute of Cell and Molecular Science, Queen Mary, University of London, London, UK. r.p.allaker@qmul.ac.uk
authors.forename: Robert P
authors.initials: RP
authors.lastname: Allaker
authors.forename: Barry E
authors.initials: BE
authors.lastname: Sheehan
authors.forename: David C
authors.initials: DC
authors.lastname: McAnerney
authors.forename: Ian J
authors.initials: IJ
authors.lastname: McKay
mesh_terms: D053607:Adrenomedullin / Q000378:metabolism / Q000494:pharmacology*; D000595:Amino Acid Sequence; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism / Q000494:pharmacology*; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000235:genetics / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000235:genetics / Q000378:metabolism; D016966:Porphyromonas gingivalis / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
chemical_list: D011135:Polysaccharides, Bacterial; D053607:Adrenomedullin; D010447:Peptide Hydrolases; D015740:Calcitonin Gene-Related Peptide