title: Characterization of interactions between human serum albumin and tumor-inhibiting amino alcohol platinum(II) complexes using capillary electrophoresis.
abstract: Platinum(II) complexes with amino alcohol ligands are of growing interest as anticancer agents capable of changing their reactivity toward biomolecules at different pH values. The binding of such compounds to the transport protein, human serum albumin (HSA), under simulated physiological conditions (pH 7.4, 100mM chloride, 37 degrees C) has been studied by capillary electrophoresis (CE), with the objective to acquire and compare their binding parameters. The association constants and stoichiometric ratios of the platinum-HSA adducts were determined by measuring the concentration changes of the peak area response of the Pt complex (after a 48 h incubation of the reaction mixture to attain equilibrium), constructing the binding isotherms, and their mathematical analysis. The investigated Pt(II) compounds were found to show moderate affinity toward HSA, with association constants ranging from 1.0 x 10(3) to 2.4 x 10(4)M(-1). Such binding behavior was attributed to a distinctive structural feature of bis(amino alcohol)platinum(II) complexes, that is, existence of an equilibrium between ring-opened and ring-closed forms in solution.
authors.affiliation: Institute of Inorganic Chemistry, University of Vienna, Waehringer Str. 42, A-1090, Vienna, Austria.
authors.forename: Svetlana S
authors.initials: SS
authors.lastname: Aleksenko
authors.forename: Christian G
authors.initials: CG
authors.lastname: Hartinger
authors.forename: Olga
authors.initials: O
authors.lastname: Semenova
authors.forename: Kristof
authors.initials: K
authors.lastname: Meelich
authors.forename: Andrei R
authors.initials: AR
authors.lastname: Timerbaev
authors.forename: Bernhard K
authors.initials: BK
authors.lastname: Keppler
mesh_terms: D000605:Amino Alcohols / Q000097:blood / Q000378:metabolism*; D000970:Antineoplastic Agents / Q000097:blood / Q000378:metabolism*; D001797:Blood Protein Electrophoresis; D019075:Electrophoresis, Capillary; D006801:Humans; D017671:Platinum Compounds / Q000097:blood / Q000378:metabolism*; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*
chemical_list: D000605:Amino Alcohols; D000970:Antineoplastic Agents; D017671:Platinum Compounds; D012709:Serum Albumin