title: The regulation of adipogenesis through GPR120.
abstract: Recently, it has been found that long-chain fatty acids activate the G protein-coupled receptors (GPRs), GPR120 and GPR40. However, there have been no reports to date on the possible physiological roles of these GPRs in adipose tissue development and adipocyte differentiation. GPR120 mRNA was highly expressed in the four different adipose tissues, and the amount of mRNA was elevated in adipose tissues of mice fed a high fat diet. However, GPR40 mRNA was not detected in any of the adipose tissues. The expression of GPR120 mRNA was higher in adipocytes compared to stromal-vascular (S-V) cells. The level of GPR120 mRNA increased during adipocyte differentiation in 3T3-L1 cells. Similar results were observed in human adipose tissue, human preadipocytes, and cultured adipocytes. Moreover, use of a small interference RNA (siRNA) to down-regulate GPR120 expression resulted in inhibition of adipocyte differentiation. Our results suggest that GPR120 regulates adipogenic processes such as adipocyte development and differentiation.
authors.affiliation: Department of Food Production Science, Faculty of Agriculture, Shinshu University, Nagano-ken 399-4598, Japan.
authors.forename: Chizu
authors.initials: C
authors.lastname: Gotoh
authors.forename: Yeon-Hee
authors.initials: YH
authors.lastname: Hong
authors.forename: Tomoyo
authors.initials: T
authors.lastname: Iga
authors.forename: Daisuke
authors.initials: D
authors.lastname: Hishikawa
authors.forename: Yasuki
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Sang-Houn
authors.initials: SH
authors.lastname: Song
authors.forename: Ki-Choon
authors.initials: KC
authors.lastname: Choi
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Adachi
authors.forename: Akira
authors.initials: A
authors.lastname: Hirasawa
authors.forename: Gozoh
authors.initials: G
authors.lastname: Tsujimoto
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Sasaki
authors.forename: Sang-Gun
authors.initials: SG
authors.lastname: Roh
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology*; D050156:Adipogenesis / Q000502:physiology*; D000818:Animals; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D043562:Receptors, G-Protein-Coupled / Q000502:physiology*
chemical_list: C498007:FFAR4 protein, mouse; D043562:Receptors, G-Protein-Coupled