title: Evaluation of the role of CYP6B cytochrome P450s in pyrethroid resistant Australian Helicoverpa armigera.
abstract: The AN02 strain of Helicoverpa armigera from eastern Australia exhibits 50-fold, PBO-suppressible resistance to the pyrethroid insecticide fenvalerate. The semidominant resistance gene RFen1 was previously mapped to AFLP Linkage Group 13. In evaluating the cytochrome P450 genes CYP6B7, CYP6B6, and CYP6B2 as candidates for RFen1, we found that they occur in a tandem array in the genome, next to the gene encoding the para-type sodium channel; the target of pyrethroid insecticides. We mapped these genes to AFLP Linkage Group 14, thus rejecting mutations within the P450 cluster or para as candidates for RFen1. RFen1 genotypes produced slightly different mRNA levels of the three P450s, but the differences were too small to convincingly account for resistance. We conclude that even if one or more of these P450s metabolize fenvalerate, they are unlikely to be responsible for the resistance in AN02.
authors.affiliation: CESAR, Department of Genetics, University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: Vladimir D
authors.initials: VD
authors.lastname: Grubor
authors.forename: David G
authors.initials: DG
authors.lastname: Heckel
mesh_terms: D000818:Animals; D001315:Australia; D001483:Base Sequence; D002874:Chromosome Mapping; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D008040:Genetic Linkage; D005838:Genotype; D007305:Insecticide Resistance / Q000235:genetics*; D007306:Insecticides / Q000494:pharmacology; D009036:Moths / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D010802:Phylogeny; D011722:Pyrethrins / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D007306:Insecticides; D011722:Pyrethrins; D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System