title: Novel expressed sequences identified in a model of androgen independent prostate cancer.
abstract: BACKGROUND
  Prostate cancer is the most frequently diagnosed cancer in American men, and few effective treatment options are available to patients who develop hormone-refractory prostate cancer. The molecular changes that occur to allow prostate cells to proliferate in the absence of androgens are not fully understood.
  
  
  RESULTS
  Subtractive hybridization experiments performed with samples from an in vivo model of hormonal progression identified 25 expressed sequences representing novel human transcripts. Intriguingly, these 25 sequences have small open-reading frames and are not highly conserved through evolution, suggesting many of these novel expressed sequences may be derived from untranslated regions of novel transcripts or from non-coding transcripts. Examination of a large metalibrary of human Serial Analysis of Gene Expression (SAGE) tags demonstrated that only three of these novel sequences had been previously detected. RT-PCR experiments confirmed that the 6 sequences tested were expressed in specific human tissues, as well as in clinical samples of prostate cancer. Further RT-PCR experiments for five of these fragments indicated they originated from large untranslated regions of unannotated transcripts.
  
  
  CONCLUSIONS
  This study underlines the value of using complementary techniques in the annotation of the human genome. The tissue-specific expression of 4 of the 6 clones tested indicates the expression of these novel transcripts is tightly regulated, and future work will determine the possible role(s) these novel transcripts may play in the progression of prostate cancer.
authors.affiliation: Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, BC, V5Z 4E6, Canada. squayle@bccrc.ca <squayle@bccrc.ca>
authors.forename: Steven N
authors.initials: SN
authors.lastname: Quayle
authors.forename: Heidi
authors.initials: H
authors.lastname: Hare
authors.forename: Allen D
authors.initials: AD
authors.lastname: Delaney
authors.forename: Martin
authors.initials: M
authors.lastname: Hirst
authors.forename: Dorothy
authors.initials: D
authors.lastname: Hwang
authors.forename: Jacqueline E
authors.initials: JE
authors.lastname: Schein
authors.forename: Steven J M
authors.initials: SJ
authors.lastname: Jones
authors.forename: Marco A
authors.initials: MA
authors.lastname: Marra
authors.forename: Marianne D
authors.initials: MD
authors.lastname: Sadar
mesh_terms: D000728:Androgens / Q000494:pharmacology; D000818:Animals; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D052138:Genes, Neoplasm; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008969:Molecular Sequence Data; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014407:Tumor Cells, Cultured; D020506:Untranslated Regions
chemical_list: D000728:Androgens; D012333:RNA, Messenger; D020506:Untranslated Regions