Suppression of mitochondrial oxidative stress provides long-term neuroprotection in experimental optic neuritis.
OBJECTIVE
Axonal loss is thought to contribute to the persistence of visual loss in optic neuritis and multiple sclerosis (MS). The mechanisms of injury are poorly understood. The authors investigated the contribution of mitochondrial oxidative stress and the effects of modulating mitochondrial antioxidant gene expression in the optic nerves of mice induced with experimental allergic encephalomyelitis (EAE), with a focus on long-term neuroprotection.


METHODS
Optic nerves from mice with EAE were probed for reactive oxygen species (ROS) with the use of dichlorofluorescein diacetate (DCFDA), dihydroethidium, and cerium chloride. To modulate mitochondrial oxidative stress, recombinant AAV containing the human SOD2 gene or a ribozyme targeting murine SOD2 was injected into the vitreous. Control eyes received the recombinant virus without a therapeutic gene. Mice were sensitized for EAE and were monitored by serial contrast-enhanced MRI. The effects of SOD2 modulation on the EAE optic nerve were gauged by computerized analysis of optic nerve volume, myelin fiber area, and retinal ganglion cell loss at 1, 3, and 12 months after sensitization for EAE.


RESULTS
ROS were detected in the EAE optic nerve as early as 3 days after antigenic sensitization. Colocalization suggested mitochondria as the source of ROS activity in the absence of inflammation. The ribozyme suppressing SOD2 gene expression increased myelin fiber injury by 27%. Increasing SOD2 levels twofold in the optic nerve by virally mediated gene transfer ameliorated myelin fiber injury by 51% and RGC loss fourfold, limiting it to 7% in EAE at 1 year.


CONCLUSIONS
Amelioration of mitochondrial oxidative stress by SOD2 gene delivery may be a therapeutic strategy for suppressing neurodegeneration in optic neuritis.
Qi
Xiaoping
X
Department of Ophthalmology, University of Florida, College of Medicine, Gainesville, FL 32610-0284, USA.
Lewin
Alfred S
AS
Sun
Liang
L
Hauswirth
William W
WW
Guy
John
J
D000818:Animals; D018929:Cell Culture Techniques; D000229:Dependovirus / Q000235:genetics; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000201:enzymology / Q000473:pathology / Q000517:prevention & control*; D019084:Fluorescent Antibody Technique, Indirect; D005456:Fluorescent Dyes; D015316:Genetic Therapy; D008279:Magnetic Resonance Imaging; D051379:Mice; D008811:Mice, Inbred DBA; D008928:Mitochondria / Q000201:enzymology*; D009186:Myelin Sheath / Q000473:pathology; D009900:Optic Nerve / Q000201:enzymology / Q000473:pathology; D009902:Optic Neuritis / Q000201:enzymology / Q000473:pathology / Q000517:prevention & control*; D018384:Oxidative Stress; D016337:RNA, Catalytic / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D012165:Retinal Ganglion Cells / Q000201:enzymology / Q000473:pathology; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D000099156:Superoxide Dismutase 2
D005456:Fluorescent Dyes; D016337:RNA, Catalytic; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase; D000099156:Superoxide Dismutase 2