[Investigation of role in antitumor immune of mouse recombinant alpha-fetoprotein conjugated with mannan].
OBJECTIVE
To investigate how to deliver AFP to the immune system for generating the appropriate immune response.


METHODS
We prepared the oxidized mannan linked to recombinant mouse alpha-fetoprotein (mAFP) as a vaccine (Ox-M-mAFP), and the vaccine based on mAFP without mannan modification was also prepared as a control.


RESULTS
We found that immunotherapy with Ox-M-mAFP was effective on protective and therapeutic antitumor immunity in mice. No marked toxicity was found in the immunized mice.


CONCLUSIONS
These findings may be important to further exploration of the breaking of immune tolerance to self molecules, and may provide a new vaccine strategy for the treatment of AFP-positive hepatocellular carcinoma.
Zhang
Wei
W
State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
Wei
Yu-Quan
YQ
Chen
Li-Juan
LJ
Liu
Ji-Yan
JY
Wu
Yang
Y
Ding
Zhen-Yu
ZY
Wen
Yan-Jun
YJ
Yang
Jin-Liang
JL
Fan
Ling-Yu
LY
Li
Jiong
J
Kan
Bing
B
Deng
Hong-Xin
HX
D000818:Animals; D019496:Cancer Vaccines / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D045744:Cell Line, Tumor; D005260:Female; D008214:Lymphocytes / Q000276:immunology; D008351:Mannans / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009369:Neoplasms / Q000276:immunology*; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism*; D000509:alpha-Fetoproteins / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism*
D019496:Cancer Vaccines; D008351:Mannans; D011994:Recombinant Proteins; D000509:alpha-Fetoproteins