title: Total phenolic contents, free radical scavenging activities and inhibitory effects on the activation of NF-kappa B of eight Centaurea L. species.
abstract: Extracts obtained from aerial parts of eight Centaurea L. (Asteraceae) species (C. calolepis, C. cariensis subsp. maculiceps, C. cariensis subsp. microlepis, C. hierapolitana, C. cadmea, C. ensiformis, C. depressa and C. urvillei subsp. urvillei), were tested for their free radical scavenging activity (FRSA) in the DPPH screening assay, for their in vitro antiinflammatory activity in the reporter gene assay for inhibition of transcription factor NF-kappaB and for their total phenolic content. Methanol extracts (0.25 mg/4 mL) of C. urvillei, C. cadmea and C. ensiformis showed strong antioxidant activity with 90.41 +/- 2.98%, 86.66 +/- 2.67% and 86.19 +/- 2.94% FRSA, respectively. Antioxidant capacity results were consistent with the total phenolic content. Chloroform extracts of C. hierapolitana, C. calolepis and C. cadmea showed strong in vitro antiinflammatory activity with IC(50) values of 2.5, 4.4 and 6.2 microg/mL, respectively.
authors.affiliation: Department of Pharmaceutical Botany, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey. canan.karamenderes@ege.edu.tr
authors.forename: Canan
authors.initials: C
authors.lastname: Karamenderes
authors.forename: Sibel
authors.initials: S
authors.lastname: Konyalioglu
authors.forename: Shabana
authors.initials: S
authors.lastname: Khan
authors.forename: Ikhlas A
authors.initials: IA
authors.lastname: Khan
mesh_terms: D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D031179:Centaurea / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017930:Genes, Reporter; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D010636:Phenols / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000893:Anti-Inflammatory Agents; D016166:Free Radical Scavengers; D016328:NF-kappa B; D010636:Phenols; D010936:Plant Extracts