A new lectin from the sea worm Serpula vermicularis: isolation, characterization and anti-HIV activity.
A GlcNAc-specific lectin was isolated from the sea worm Serpula vermicularis (SVL) (Annelida) and purified by ion-exchange, affinity and gel permeation chromatography. SVL was a homotetrameric protein with native molecular mass of about 50 kDa, and consisted of identical subunits of 12.7 kDa. The carbohydrate content of 1.9% suggested that the lectin was a glycoprotein, and mainly composed by aspartic and glutamic acids, glycine, valine and serine; with relatively lower content of basic amino acids and cysteine. The first 15 residues of the N-terminal region were determined as ADTPCQMLGSRYGWR. It was stable at pH 6-9 and at temperatures up to 40 degrees C. SVL was Ca(2+)-independent lectin that agglutinated native and trypsinized human erythrocytes. Hapten inhibition studies indicated that SVL showed binding specificity only for N-acetyl-d-glucosamine and its derivatives among the monosaccharides tested and required the presence of hydroxyl group at the C-3 of GlcNAc. The presence of hydrophobic p-nitrophenyl aglycone improved inhibitory potency of N-acetyl-d-glucosamine. Ovomucoid and ovalbumin were found to be inhibitors among the glycoproteins used for inhibition assay. The anti-HIV-1 (human immunodeficiency virus) activity of SVL in vitro was determined: SVL inhibited the production of viral p24 antigen and cytopathic effect induced by HIV-1. The EC(50) values were 0.23 and 0.15 microg x mL(-1) respectively.
Molchanova
Valentina
V
Laboratory of Chemistry of Non-Infectious Immunity of Pacific Institute of Bioorganic Chemistry Far East Branch of the Russian Academy of Sciences, Vladivostok, Russia. molchanova@piboc.dvo.ru
Chikalovets
Irina
I
Chernikov
Oleg
O
Belogortseva
Natalia
N
Li
Wei
W
Wang
Jian-Hua
JH
Yang
Dong-Yun Ou
DY
Zheng
Yong-Tang
YT
Lukyanov
Pavel
P
D000371:Agglutination / Q000187:drug effects; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D019380:Anti-HIV Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects; D015497:HIV-1 / Q000187:drug effects; D006801:Humans; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D011077:Polychaeta
D000596:Amino Acids; D019380:Anti-HIV Agents; D037102:Lectins