Exogenous H(2)O(2) and catalase treatments interfere with Tri genes expression in liquid cultures of Fusarium graminearum.
Effect of exogenous H(2)O(2) and catalase was tested in liquid cultures of the deoxynivalenol and 15-acetyldeoxynivalenol-producing fungus Fusarium graminearum. Accordingly to previous results, H(2)O(2) supplementation of the culture medium leads to increased toxin production. This study indicates that this event seems to be linked to a general up regulation of genes involved in the deoxynivalenol and 15-acetyldeoxynivalenol biosynthesis pathway, commonly named Tri genes. In catalase-treated cultures, toxin accumulation is reduced, and Tri genes expression is significantly down regulated. Furthermore, kinetics of expression of several Tri genes is proposed in relation to toxin accumulation. Biological meanings of these findings are discussed.
Ponts
Nadia
N
INRA Centre de Bordeaux, UPR1264 MycSA, 71 Avenue Edouard Bourleaux, BP81, 33883 Villenave d'Ornon Cedex, France.
Pinson-Gadais
Laetitia
L
Barreau
Christian
C
Richard-Forget
Florence
F
Ouellet
Thérèse
T
D001483:Base Sequence; D002374:Catalase / Q000494:pharmacology*; D004271:DNA, Fungal / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005670:Fusarium / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D005800:Genes, Fungal / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007700:Kinetics; D008954:Models, Biological; D018384:Oxidative Stress; D014255:Trichothecenes / Q000096:biosynthesis / Q000235:genetics
D004271:DNA, Fungal; D005656:Fungal Proteins; D014255:Trichothecenes; C046760:15-acetyldeoxynivalenol; D006861:Hydrogen Peroxide; D002374:Catalase; C007262:deoxynivalenol