[Inheritance instability of chromosome 6q in patients with gastric cancer].
OBJECTIVE
To investigate the possible correlation between the microsatellite DNA instability (MSI), loss of heterozygosity (LOH ) on the long arm of chromosome 6 (6q) and the clinicopathologic features in Chinese patients with gastric cancer.


METHODS
PCR-SSLP-Silver Stain method was used to detect four loci MSI and LOH in gastric tumor and paired normal control tissues of twenty and seven cases.


RESULTS
In our study, the positive of MSI at one or more loci were observed in 16 out of 27 informative individuals (59.2%). 7 cases of 11 informative individuals without MSI were detected to happen the LOH at one or more loci (63.6%). And both MSI and LOH were frequently resulting at loci D6S434 (6q16.3-q21) and D6S404 (6q16.3-q23.2). MSI and LOH occurred in both well-differentiated and poor- differentiated gastric cancers, with a slight tendency of high grade MSI (MSI-H) existing chiefly in well-differentiated gastric cancer.


CONCLUSIONS
The existence of MSI and/or LOH on 6q in 80% gastric carcinoma suggests that the genetic instability of 6q plays an important role in Chinese gastric cancer. The frequency of MSI and LOH has no significant relationship to clinicopathologic features such as age, gender, histological grade of differentiation and stages of tumor. The critical region of allelic deletion on 6q in Chinese gastric cancer is similar to those in other countries, and further confirms the presence of a putative tumor suppressor gene in this region.
Chen
Ping
P
Department of Genetics and Molecule Biology, Xi'an Jiaotong University School of Medicine, Xi'an 710061, China.
Chu
Yong-Lie
YL
Song
Tu-Sheng
TS
Lei
Li
L
Wang
Jian-Sheng
JS
Wang
Kang
K
Ni
Lei
L
Niu
Yue-Ying
YY
D000328:Adult; D000368:Aged; D043171:Chromosomal Instability; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics*; D042241:Early Diagnosis; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D040582:Inheritance Patterns / Q000235:genetics*; D019656:Loss of Heterozygosity; D008297:Male; D053842:Microsatellite Instability; D008875:Middle Aged; D016622:Silver Staining; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology / Q000628:therapy