Identification of flux regulation coefficients from elementary flux modes: A systems biology tool for analysis of metabolic networks.
Within a metabolic network, the elementary flux modes enables a unique description of different operations of the network. Thus, the metabolic fluxes can be specified as convex combinations of the elementary flux modes. Here, we describe an approach to identify the set of elementary flux modes that operates in a given metabolic network through the use of measurements of macroscopic fluxes, that is, fluxes in and out of the cell. Besides enabling estimation of the metabolic fluxes, the parameters of the linear combinations of the elementary flux modes provide valuable physiological information; we call these parameters flux regulation coefficients (FRCs). These coefficients indicate which enzyme subsets are important at different growth conditions. We demonstrate how FRCs can be used to map the operation of the metabolic network of the yeast Saccharomyces sp. under different growth conditions.
Nookaew
Intawat
I
Department of Chemical Engineering, King Mongkut's University of Technology Thonburi, Bangkok 10140, Thailand.
Meechai
Asawin
A
Thammarongtham
Chinae
C
Laoteng
Kobkul
K
Ruanglek
Vasimon
V
Cheevadhanarak
Supapon
S
Nielsen
Jens
J
Bhumiratana
Sakarindr
S
D049109:Cell Proliferation; D003198:Computer Simulation; D004735:Energy Transfer / Q000502:physiology; D008954:Models, Biological; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D012984:Software; D049490:Systems Biology / Q000379:methods
D029701:Saccharomyces cerevisiae Proteins