title: Identification of a necessary element for Toxoplasma gondii SAG1 gene expression.
abstract: SAG1 codes for the stage-specific major surface antigen P30 of Toxoplasma gondii (T. gondii) tachyzoites. Six tandemly repeated, conserved 27 bp cassettes in the region from -231 to -70 bp were previously confirmed to be essential for high-level expression of SAG1 and serve as a positioning element directing the initiation of transcription. We demonstrate here that an element located between +19 and +28 bp is necessary for SAG1 gene expression by using deletion mutagenesis analysis and electrophoresis mobility shift assay (EMSA). This will provide an insight into the regulatory mechanisms of SAG1 gene expression.
authors.affiliation: Department of Parasitology, School of Medicine, Shandong University, and Nephrology Department, The Second Hospital of Shandong University, PR China.
authors.forename: Jiaqin
authors.initials: J
authors.lastname: Zhang
authors.forename: Qinmin
authors.initials: Q
authors.lastname: Gu
authors.forename: Xianghua
authors.initials: X
authors.lastname: Hou
authors.forename: Huaiyu
authors.initials: H
authors.lastname: Zhou
authors.forename: Hua
authors.initials: H
authors.lastname: Cong
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Qunli
authors.initials: Q
authors.lastname: Zhao
authors.forename: Shouqing
authors.initials: S
authors.lastname: Li
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000096:biosynthesis / Q000235:genetics*; D000954:Antigens, Surface / Q000096:biosynthesis / Q000235:genetics; D015342:DNA Probes / Q000737:chemistry; D024202:Electrophoretic Mobility Shift Assay; D005786:Gene Expression Regulation / Q000502:physiology*; D005822:Genetic Vectors; D006801:Humans; D008156:Luciferases / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D011401:Promoter Regions, Genetic / Q000235:genetics; D015800:Protozoan Proteins / Q000096:biosynthesis / Q000235:genetics*; D016053:RNA, Protozoan / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020080:Tandem Repeat Sequences / Q000502:physiology; D014122:Toxoplasma / Q000235:genetics* / Q000276:immunology; D014162:Transfection
chemical_list: D000953:Antigens, Protozoan; D000954:Antigens, Surface; D015342:DNA Probes; D015800:Protozoan Proteins; D016053:RNA, Protozoan; C047168:SAG1 antigen, Toxoplasma; D008156:Luciferases