Myosin specific-T lymphocytes mediated myocardial inflammation in adoptive transferred rats.
Myosin specific-T lymphocytes might mediate myocardial inflammation and remodeling after AMI. Myosin-activated or unactivated T lymphocytes in vitro were transferred into naive syngeneic rats, respectively. T lymphocyte infiltration and myocyte apoptosis were explored by the HE and TUNNEL. Proteins and mRNA levels of cytokines (IL-1beta, IL-6 and TNF-alpha) in myocardium were determined by RT-PCR and immunohisto-chemistry. T lymphocyte infiltration was evidently observed after one week of activated T cell transfer. The expressions of cytokines were elevated markedly one week later. The myocyte apoptosis occurred after T lymphocyte infiltration in myocardium. Our findings suggest that cardiac myosin activated-T lymphocytes may mediate myocardial inflammation and remodeling.
Zhang
Jin
J
Laboratory of Cardiovascular Immunology, Institute of Cardiology, Union Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, China.
Liao
Yuhua
Y
Cheng
Xiang
X
Chen
Jing
J
Chen
Peng
P
Gao
Xiang
X
Zhang
Zhengjenny
Z
D019264:Adoptive Transfer; D000818:Animals; D017209:Apoptosis / Q000276:immunology; D009205:Myocarditis / Q000276:immunology*; D009206:Myocardium / Q000276:immunology*; D032383:Myocytes, Cardiac / Q000276:immunology; D009218:Myosins / Q000276:immunology*; D051381:Rats; D011917:Rats, Inbred Lew; D013601:T-Lymphocytes / Q000276:immunology*
D009218:Myosins