Expression of the IFN-inducible p53-target gene TRIM22 is down-regulated during erythroid differentiation of human bone marrow.
The interferon inducible protein TRIM22 has been identified as a p53 target gene, with possible involvement in proliferation and differentiation of leukaemia cells. Here, the expression levels of TRIM22 during haematopoietic differentiation are characterised. Expression of TRIM22 correlates inversely to differentiation, as TRIM22 is highly expressed in CD34(+) human bone marrow progenitor cells, but declines in mature populations. The erythroid lineage appears as a special case, as TRIM22 expression shows an extreme decrease during late erythroid maturation and is completely undetectable in nucleated erythroid populations in contrast to other lineages. In conclusion, our data could suggest lineage-specific roles for TRIM22 during haematopoietic differentiation.
Obad
Susanna
S
Department of Haematology and Transfusion Medicine, BioMedical Centre, Lund University, Lund, Sweden. Susanna.Obad@med.lu.se
Olofsson
Tor
T
Mechti
Nadir
N
Gullberg
Urban
U
Drott
Kristina
K
D018952:Antigens, CD34 / Q000378:metabolism; D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D015536:Down-Regulation; D041905:Erythroid Cells / Q000166:cytology*; D005434:Flow Cytometry; D015870:Gene Expression; D006098:Granulocytes / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007372:Interferons / Q000494:pharmacology; D015778:Minor Histocompatibility Antigens; D012333:RNA, Messenger / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics*; D000070584:Tripartite Motif Proteins
D018952:Antigens, CD34; D015778:Minor Histocompatibility Antigens; D012333:RNA, Messenger; D012097:Repressor Proteins; C094314:TRIM22 protein, human; D014157:Transcription Factors; D000070584:Tripartite Motif Proteins; D007372:Interferons