title: Use of controlled ovulation of the dominant follicle to assess oocyte maturation during natural menstrual cycles in rhesus macaques.
abstract: To determine the practicality of controlled ovulation of the dominant follicle as a technique to study meiotic maturation of oocytes during contraceptive research, we developed a technique for aspiration of the single dominant follicle using a dual-needle continuous irrigation technique 27 hours after an ovulatory stimulus. All of the oocytes (3/3) recovered from control animals, but only 1/6 (17%) of oocytes from animals treated with the meiotic inhibitor ORG 9935 exhibited germinal vesicle breakdown, indicating resumption of meiosis.
authors.affiliation: Department of Obstetrics & Gynecology, Oregon Health & Science University, Portland, Oregon 97239, USA. jensenje@ohsu.edu
authors.forename: Jeffrey T
authors.initials: JT
authors.lastname: Jensen
authors.forename: Jessica E
authors.initials: JE
authors.lastname: Stanley
authors.forename: Mary B
authors.initials: MB
authors.lastname: Zelinski
authors.forename: Richard L
authors.initials: RL
authors.lastname: Stouffer
authors.forename: Darla
authors.initials: D
authors.lastname: Jacob
authors.forename: John
authors.initials: J
authors.lastname: Fanton
mesh_terms: D000818:Animals; D005260:Female; D008253:Macaca mulatta; D008597:Menstrual Cycle / Q000502:physiology*; D009865:Oocytes / Q000502:physiology*; D006080:Ovarian Follicle / Q000502:physiology*; D010062:Ovulation Induction; D012016:Reference Values