Gas chromatography-mass spectrometric assay for propofol in cerebrospinal fluid of traumatic brain patients.
A sensitive gas chromatography-mass spectrometry method for measuring propofol in cerebrospinal fluid is described, validated and applied to four patients after traumatic brain injury. The limit of quantitation was 2 microg/L using a volume of 0.5 mL. The inter- and intra-assay coefficients of variation were 5.9 and 5.1%, respectively. The assay covers the linear concentration range of 2-50 microg/L, which is relevant in clinical pharmacokinetic studies when propofol is used in the Intensive Care Unit as a sedative agent or to lower the intracranial pressure.
Peeters
Mariska Y M
MY
Department of Clinical Pharmacy, St. Antonius Hospital, P.O. Box 2500, 3430 EM Nieuwegein, The Netherlands. r.peeters@antoniusmesosgroep.nl
Kuiper
Hiltjo
H
Greijdanus
Ben
B
van der Naalt
Joukje
J
Knibbe
Catherijne A J
CA
Uges
Donald R A
DR
D000293:Adolescent; D000328:Adult; D018686:Anesthetics, Intravenous / Q000134:cerebrospinal fluid*; D001930:Brain Injuries / Q000134:cerebrospinal fluid*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008297:Male; D015742:Propofol / Q000134:cerebrospinal fluid*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D018686:Anesthetics, Intravenous; D015742:Propofol