title: The inhibitory binding site(s) of Zn2+ in cytochrome c oxidase.
abstract: EXAFS analysis of Zn binding site(s) in bovine-heart cytochrome c oxidase and characterization of the inhibitory effect of internal zinc on respiratory activity and proton pumping of the liposome reconstituted oxidase are presented. EXAFS identifies tetrahedral coordination site(s) for Zn(2+) with two N-histidine imidazoles, one N-histidine imidazol or N-lysine and one O-COOH (glutamate or aspartate), possibly located at the entry site of the proton conducting D pathway in the oxidase and involved in inhibition of the oxygen reduction catalysis and proton pumping by internally trapped zinc.
authors.affiliation: Department of Biology, University of Bologna, Bologna, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Francia
authors.forename: Lisa
authors.initials: L
authors.lastname: Giachini
authors.forename: Federico
authors.initials: F
authors.lastname: Boscherini
authors.forename: Giovanni
authors.initials: G
authors.lastname: Venturoli
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Capitanio
authors.forename: Pietro Luca
authors.initials: PL
authors.lastname: Martino
authors.forename: Sergio
authors.initials: S
authors.lastname: Papa
mesh_terms: D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D002417:Cattle; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D004579:Electron Transport / Q000187:drug effects; D003576:Electron Transport Complex IV / Q000037:antagonists & inhibitors* / Q000737:chemistry* / Q000378:metabolism; D006736:Horses; D008081:Liposomes; D008958:Models, Molecular; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D017494:Proton Pumps / Q000187:drug effects; D011522:Protons; D015032:Zinc / Q000378:metabolism* / Q000494:pharmacology*
chemical_list: D008081:Liposomes; D021122:Protein Subunits; D017494:Proton Pumps; D011522:Protons; D003576:Electron Transport Complex IV; D015032:Zinc