Influence of simvastatin on bone regeneration of tibial defects and blood cholesterol level in rats.
The purpose of this study was to evaluate the effects of simvastatin, by oral or subcutaneous administration, on tibial defects regeneration and blood cholesterol level in rats. A surgical defect was made on the right tibia of 40 male animals assigned to 4 groups (n=10), based on two routes of administration and on the use or not of simvastatin: subcutaneous injection of simvastatin (7 mg/kg) (group AT) or only the vehicle of drug suspension (group AC), above the defect area, for 5 days; and 20 mg/kg of simvastatin macerated on water (group BT) or only water (group BC), orally, daily, during the whole observation period. The animals were sacrificed after 15 or 30 days, when blood samples were analyzed to check plasma cholesterol levels. Tibiae were removed and, after decalcification and routine laboratorial processing, histological and histomorphometrical analyses were carried out. ANOVA was used for statistical analysis at 5% significance level. The histological and histomorphometrical analyses showed significant differences only between the experimental periods (p<0.05). Animals sacrificed after 30 days showed better bone repair (p<0.05). There was no statistically significant difference (p>0.05) for blood cholesterol levels between the groups. In conclusion, simvastatin administration either orally or subcutaneously did not improve bone repair of experimental tibial defects and did not alter blood cholesterol levels in rats.
Anbinder
Ana Lia
AL
Department of Dentistry, University of Taubaté, Rua dos Operários 9, 12020-270 Taubaté, SP, Brazil. anaanbinder@hotmail.com
Junqueira
Juliana Campos
JC
Mancini
Maria Nadir G
MN
Balducci
Ivan
I
Rocha
Rosilene Fernandes da
RF
Carvalho
Yasmin Rodarte
YR
D000284:Administration, Oral; D000818:Animals; D000924:Anticholesteremic Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D001847:Bone Diseases / Q000188:drug therapy* / Q000473:pathology; D001861:Bone Regeneration / Q000187:drug effects*; D016723:Bone Remodeling / Q000187:drug effects; D002784:Cholesterol / Q000097:blood*; D002863:Chromogenic Compounds; D007279:Injections, Subcutaneous; D008297:Male; D010006:Osteoblasts / Q000187:drug effects / Q000473:pathology; D010011:Osteocytes / Q000187:drug effects / Q000473:pathology; D010012:Osteogenesis / Q000187:drug effects; D010521:Periosteum / Q000187:drug effects / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D019821:Simvastatin / Q000008:administration & dosage / Q000627:therapeutic use*; D013977:Tibia / Q000187:drug effects* / Q000473:pathology; D013997:Time Factors
D000924:Anticholesteremic Agents; D002863:Chromogenic Compounds; D002784:Cholesterol; D019821:Simvastatin