title: Effect of carbon dioxide pneumoretroperitoneum on bacterial translocation in an experimental retroperitoneoscopy model.
abstract: OBJECTIVE
  To evaluate the effect of artificial CO2 pneumoretroperitoneum on bacterial translocation in an experimental retroperitoneoscopy model.
  
  
  METHODS
  Eighteen adult male New Zealand White rabbits weighing 2.5 to 3 kg were divided into two groups. Group 1 (control group) consisted of 6 rabbits, while the remaining 12 served as the pneumoretroperitoneum group (group 2). In group 1, the left retroperitoneal space was dissected with a 50-mL balloon without CO2 insufflation, and the animals were kept under anesthesia for 3 hours with the balloons inflated. In group 2, after balloon dissection as in group 1, CO2 insufflation was applied at 1 L/min to achieve a pressure of 10 to 12 mm Hg for 3 hours. Afterward, all animals were sacrificed, and samples were taken from the blood, retroperitoneal area, lungs, liver, mesentery, heart, kidneys, ureters, bladder, colon, small intestine, and spleen and carried to the microbiology laboratory in Carry-Blair medium. Bacterial growth was evaluated using standard techniques.
  
  
  RESULTS
  All animals survived the experimental procedures. None of the rabbits in the control group demonstrated any bacterial translocation in the sampled tissues. In the pneumoretroperitoneum group, one rabbit was found to have 10(2) colony-forming units of E. coli in the kidney, but this was considered to be the result of contamination, not translocation.
  
  
  CONCLUSIONS
  Carbon dioxide pneumoretroperitoneum does not seem to cause bacteremia or bacterial translocation in this experimental model. Retroperitoneoscopy probably does not create any additional risk of septic complications.
authors.affiliation: Department of Urology, Afyon Kocatepe University School of Medicine, Afyonkarahisar, Turkey. emretuzel@aku.edu.tr
authors.forename: Emre
authors.initials: E
authors.lastname: Tüzel
authors.forename: Cem
authors.initials: C
authors.lastname: Güler
authors.forename: Orhan Cem
authors.initials: OC
authors.lastname: Aktepe
authors.forename: Kadir
authors.initials: K
authors.lastname: Saritaş
authors.forename: M Murat
authors.initials: MM
authors.lastname: Samli
authors.forename: Murat
authors.initials: M
authors.lastname: Demirbaş
mesh_terms: D000818:Animals; D018988:Bacterial Translocation / Q000187:drug effects*; D002245:Carbon Dioxide / Q000494:pharmacology*; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000502:physiology*; D008297:Male; D011817:Rabbits; D012188:Retropneumoperitoneum / Q000139:chemically induced* / Q000382:microbiology*
chemical_list: D002245:Carbon Dioxide