Killer Phenomenon in USTILAGO MAYDIS: The Organization of the Viral Genome.
The double-stranded RNA content, the production of inactive killer protein, and the presence of virus-like particles were examined in induced nonkiller mutants and nonkiller progeny from a cross between a killer strain and a sensitive strain. A correlation between the loss of the 0.7 x 10(6) daltons dsRNA of the Ustilago maydis P6 virus and the lack of synthesis of the killer protein was established. In vitro and in vivo complementation between nonkiller strains provide additional support for the suggestion that the 0.7 x 10(6) daltons dsRNA is related to the killer function. The coding capacity of the various species of dsRNA is discussed in relation to their possible function.
Koltin
Y
Y
Department of Microbiology, Faculty of Life Sciences, Tel-Aviv University, Ramat-Aviv, Israel.
Kandel
J S
JS