Flow injection chemiluminescence determination of sudan I in hot chilli sauce.
A chemiluminescence method based on the luminol-H2O2 system with flow injection technology was proposed for the determination of sudan I in hot chilli sauce. It was found that sudan I could enhance chemiluminescence intensity generated from the luminol-H2O2 system. The increment of chemiluminescence intensity was proportional to the concentration of sudan I, giving a calibration graph linear over the concentration from 10 pg mL-1 to 7 ng mL-1 (R 2 = 0.9980) with the detection limit of 3 pg mL-1 (3sigma) and the quantification limit of 7.5 pg mL-1. At a flow rate of 2.0 mL min-1, one analysis cycle, including sampling and washing, could be accomplished in 60 s with a relative standard deviation of <5.0%. The method has been applied successfully to the determination of sudan I in Pixian douban, Golden Mark guilin chilli sauce, and Golden Mark satay sauce, and the recovery was 90.6-110.0%.
Liu
Yanhong
Y
Department of Chemistry, Northwest University, 710069 Xi'an, China.
Song
Zhenghua
Z
Dong
Faxin
F
Zhang
Lin
L
D002212:Capsicum / Q000737:chemistry; D002273:Carcinogens / Q000032:analysis*; D003212:Condiments / Q000032:analysis*; D017022:Flow Injection Analysis; D005505:Food Coloring Agents / Q000032:analysis*; D006861:Hydrogen Peroxide; D008163:Luminescent Measurements; D008165:Luminol; D009284:Naphthols / Q000032:analysis*
D002273:Carcinogens; D005505:Food Coloring Agents; D009284:Naphthols; C024336:1-phenylazo-2-naphthol; D008165:Luminol; D006861:Hydrogen Peroxide