title: Loss of SHP-1 tyrosine phosphatase expression correlates with the advanced stages of cutaneous T-cell lymphoma.
abstract: Cutaneous T-cell lymphoma (CTCL) comprises distinct and often progressive stages of skin involvement by patches, plaques, and tumors. We have previously demonstrated that CTCL-derived malignant T-cell lines display loss of a tumor suppressor SHP-1 tyrosine phosphatase because of epigenetic silencing of its gene. The silencing is induced by an activated phosphorylated (p)-STAT3 transcription factor in cooperation with DNA methyltransferase 1 (DNMT1), the key member of the epigenetic gene silencing machinery. To determine at which stage of CTCL the loss of SHP-1 occurs and how it correlates with the expression of (p)-STAT3 and DNMT1, we examined by immunohistochemistry 47 formalin-fixed skin biopsies from various stages of CTCL. Six pairs of the biopsies were obtained before and after CTCL progression at the patch or plaque and tumor stage, respectively. In 5 of these pairs, we identified loss of SHP-1 expression in atypical lymphocytes at the tumor stage; less prominent SHP-1 loss was noted in 3 biopsies from the earlier stage. The SHP-1 loss was also observed in 5 of 6 tumor, 12 of 18 plaque, and only 2 of 11 patch stages in patients with single biopsies. The expression of (p)-STAT3 and DNMT1 could be identified in almost all cases in at least a subset of the lesional cells. Based on these findings, we postulate that expression of (p)-STAT3 and DNMT1 occurs at the early stages of CTCL, and that this expression alone seems insufficient to induce loss of SHP-1 expression. In turn, SHP-1 loss correlates with, and may contribute to, progression of CTCL.
authors.affiliation: Department of Pathology, Jefferson Medical College, Philadelphia, PA 19107, USA.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Witkiewicz
authors.forename: Puthiyaveettil
authors.initials: P
authors.lastname: Raghunath
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Wasik
authors.forename: Jacqueline M
authors.initials: JM
authors.lastname: Junkins-Hopkins
authors.forename: Dan
authors.initials: D
authors.lastname: Jones
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Niels
authors.initials: N
authors.lastname: Odum
authors.forename: Mariusz A
authors.initials: MA
authors.lastname: Wasik
mesh_terms: D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000096:biosynthesis; D020868:Gene Silencing; D006801:Humans; D007150:Immunohistochemistry; D016410:Lymphoma, T-Cell, Cutaneous / Q000473:pathology / Q000503:physiopathology*; D009182:Mycosis Fungoides / Q000473:pathology / Q000503:physiopathology; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6 / Q000096:biosynthesis*; D050796:STAT3 Transcription Factor / Q000096:biosynthesis; D012878:Skin Neoplasms / Q000473:pathology / Q000503:physiopathology*
chemical_list: D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases; C000619380:DNMT1 protein, human; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6