title: Mass Spectral Evidence of the Occurrence of Vindoline in Heterotrophic Cultures of Catharanthus roseus Cells.
abstract: Vindoline concentrations in the leaves of 70 CATHARANTHUS ROSEUS of 3 cultivars were analyzed by HPLC, and 3 plants were selected for starting callus cultures on different media. When the initial calli were analyzed using a vindoline-specific RIA, the assay suggested a vindoline content of about 10 (-5)% dry weight for one-third of the first 60 cultures examined. Due to the unexpectedly high incidence of vindoline-positive calli, the screening programme was discontinued and efforts were concentrated on verifying the existence of this alkaloid in the cells. Suspension cultures derived from the 5 most immunopositive calli in an alkaloid production medium were analyzed by HPLC and GC/MS. Comparison with reference material showed that the heterotrophic suspension cultures contained a compound identical to vindoline.
authors.affiliation: Department of Pharmaceutical Chemistry, University of Kuopio, FOB 6, SF-70211 Kuopio, Finland.
authors.forename: T
authors.initials: T
authors.lastname: Naaranlahti
authors.forename: S P
authors.initials: SP
authors.lastname: Lapinjoki
authors.forename: A
authors.initials: A
authors.lastname: Huhtikangas
authors.forename: L
authors.initials: L
authors.lastname: Toivonen
authors.forename: U
authors.initials: U
authors.lastname: Kurtén
authors.forename: V
authors.initials: V
authors.lastname: Kauppinen
authors.forename: M
authors.initials: M
authors.lastname: Lounasmaa