The imprinted H19 noncoding RNA is a primary microRNA precursor.
Although H19 was the first imprinted noncoding transcript to be identified, the function of this conserved RNA has remained unclear. Here, we identify a 23-nucleotide microRNA derived from H19 that is endogenously expressed in human keratinocytes and neonatal mice and overexpressed in cells transfected with human or mouse H19 expression plasmids. These data demonstrate that H19 can function as a primary microRNA precursor and suggest that H19 expression results in the post-transcriptional downregulation of specific mRNAs during vertebrate development.
Cai
Xuezhong
X
Center for Virology and Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA.
Cullen
Bryan R
BR
D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D018507:Gene Expression Regulation, Developmental; D018392:Genomic Imprinting; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism*; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated / Q000235:genetics / Q000378:metabolism*
C094335:H19 long non-coding RNA; D035683:MicroRNAs; D012322:RNA Precursors; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated