[Isolation, identification and characteristics of a fenpropathrin-degrading bacterium JQL4-5].
A bacterium capable of utilizing fenpropathrin as sole carbon source was isolated from activated sludge collected from wastewater treating system of a pesticide manufacturer. This bacterium was identified as Sphingomonas sp. according to its physiological & biochemical analysis and the similarity analysis of its 16S rDNA sequence (GenBank Accession No. DQ177525). This bacterium could degrade 99.8% of 20 mg/L fenpropathrin in 24h. The optimal pH and temperature for the degradation were 7.0 and 30 degrees C, respectively. The degradation speed was related positively to initial inoculum size. The enzyme distribution experiment showed that the degrading-enzyme in the bacterium was endoenzyme.
Hong
Yuan-fan
YF
Key Laboratory of Microbiological Engineering Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China. hyf152003@yahoo.com.cn
Hong
Qing
Q
Wu
Jun
J
Zhang
Zhong-hui
ZH
Li
Shun-peng
SP
D001673:Biodegradation, Environmental; D006863:Hydrogen-Ion Concentration; D007306:Insecticides / Q000378:metabolism; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010802:Phylogeny; D011722:Pyrethrins / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D020578:Sphingomonas / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D013696:Temperature
D007306:Insecticides; D011722:Pyrethrins; D012336:RNA, Ribosomal, 16S; C044267:fenpropathrin