Reporter gene imaging of protein-protein interactions in living subjects.
In the past few years there has been a veritable explosion in the field of reporter gene imaging, with the aim of determining the location, duration and extent of gene expression within living subjects. An important application of this approach is the molecular imaging of interacting protein partners, which could pave the way to functional proteomics in living animals and might provide a tool for the whole-body evaluation of new pharmaceuticals targeted to modulate protein-protein interactions. Three general methods are currently available for imaging protein-protein interactions in living subjects using reporter genes: a modified mammalian two-hybrid system, a bioluminescence resonance energy transfer (BRET) system, and split reporter protein complementation and reconstitution strategies. In the future, these innovative approaches are likely to enhance our appreciation of entire biological pathway systems and their pharmacological regulation.
Massoud
Tarik F
TF
Department of Radiology, University of Cambridge School of Clinical Medicine, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QQ, UK. tfm23@radiol.cam.ac.uk <tfm23@radiol.cam.ac.uk>
Paulmurugan
Ramasamy
R
De
Abhijit
A
Ray
Pritha
P
Gambhir
Sanjiv S
SS
D000818:Animals; D015870:Gene Expression; D017930:Genes, Reporter; D008156:Luciferases / Q000378:metabolism; D008163:Luminescent Measurements; D011506:Proteins / Q000378:metabolism*
D011506:Proteins; D008156:Luciferases