title: Temporal trends in the incidence of HIV infection in antenatal clinic attendees in Addis Ababa, Ethiopia, 1995-2003.
abstract: BACKGROUND
  The HIV incidence data are relevant in depicting the current dynamics and trend of the epidemic. Using a new laboratory method for HIV-1 incidence, we aimed at estimating a 10-year trend in HIV-1 incidence in Addis Ababa, Ethiopia.
  
  
  METHODS
  We determined the temporal trends in HIV incidence based on a total of 7744 serum specimens from pregnant women who attended antenatal clinics in Addis Ababa between 1995 and 2003. HIV incidence was determined by IgG-capture HIV-1 BED incidence enzyme immunoassay following a validation using a well-characterized panel of serial serum specimens from subtype C-infected seroconverters.
  
  
  RESULTS
  Of the 1350 HIV+ specimens tested as part of the annual sentinel survey between 1995 and 2003, a total of 1332 (98.7%) were tested by BED HIV-1 incidence assay. The incidence rate of HIV-1 infection declined significantly from 7.7% (95% CI, 3.9-11.5%) in 1995 to 2.0% (95% CI, 0.7-3.3%) in 2003. Although there was a trend, amongst the age group of 15-29 years, in age-specific decline in incidence, it was not statistically significant. No change in HIV incidence rate was observed for the group aged above 30 years.
  
  
  CONCLUSIONS
  A corresponding decline in the incidence of HIV infection was observed with the decline in the prevalence of HIV infection between 1995 and 2003 in Addis Ababa City. Whether the declines were because of changes in sexual behaviours or other reasons needs to be explored. The BED HIV-1 incidence assay provides a valuable tool in obtaining information on recent HIV-1 infection.
authors.affiliation: Ethiopian Health and Nutrition Research Institute (EHNRI)-Centers for Disease Control and Prevention (CDC) Programme on AIDS, STI and Tuberculosis, Addis Ababa, Ethiopia. d_wolday@yahoo.com
authors.forename: D
authors.initials: D
authors.lastname: Wolday
authors.forename: H
authors.initials: H
authors.lastname: Meles
authors.forename: E
authors.initials: E
authors.lastname: Hailu
authors.forename: T
authors.initials: T
authors.lastname: Messele
authors.forename: Y
authors.initials: Y
authors.lastname: Mengistu
authors.forename: M
authors.initials: M
authors.lastname: Fekadu
authors.forename: B S
authors.initials: BS
authors.lastname: Parekh
authors.forename: T
authors.initials: T
authors.lastname: Wuhib
mesh_terms: D000293:Adolescent; D000328:Adult; D017677:Age Distribution; D005002:Ethiopia / Q000453:epidemiology; D005260:Female; D015658:HIV Infections / Q000453:epidemiology*; D006801:Humans; D015994:Incidence; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000453:epidemiology*; D015995:Prevalence; D013997:Time Factors