title: Interaction of ovalbumin with phospholipids Langmuir-Blodgett film.
abstract: Interaction of native ovalbumin (OVA) with 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) Langmuir-Blodgett monolayer has been studied at the air-water interface. A compressibility study shows the positive association with DPPC. Adsorption kinetics shows that the protein adsorption is a one-step process and the amount of protein adsorbed depends on the concentration of protein at the water subphase. Incorporation of protein into the DPPC layer is surface-pressure dependent. The compressibility study indicates that the DPPC-OVA interaction is hydrophobic in nature and structural reorganization is eminent to adjust the hydrophobic mismatch between DPPC acyl chains and OVA hydrophobic moieties. At higher pressure, OVA tends to squeeze out from the DPPC monolayer. A nanometer scale FE-SEM image confirms this observation. Globular aggregates of protein of dimension 60-80 nm were observed in DPPC-OVA supported film. Steady-state fluorescence spectroscopy suggests that the tryptophan residues of OVA are main emitting species. The blue shift of tryptophan fluorescence in supported film may be due to the tryptophan molecule of protein exposed to the hydrophobic air phase.
authors.affiliation: Department of Spectroscopy, Indian Association for the Cultivation of Science, Jadavpur, Kolkata--700032, India.
authors.forename: Tapanendu
authors.initials: T
authors.lastname: Kamilya
authors.forename: Prabir
authors.initials: P
authors.lastname: Pal
authors.forename: G B
authors.initials: GB
authors.lastname: Talapatra
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D000327:Adsorption; D001665:Binding Sites; D007700:Kinetics; D008567:Membranes, Artificial; D010047:Ovalbumin / Q000737:chemistry*; D010316:Particle Size; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D013499:Surface Properties
chemical_list: D008567:Membranes, Artificial; D015060:1,2-Dipalmitoylphosphatidylcholine; D010047:Ovalbumin