Plasma level of stromal derived factor-1 (SDF-1) is increased in disseminated intravascular coagulation patients who have poor outcomes: in vitro effect of SDF-1 on coagulopathy.
Stromal cell-derived factor-1 (SDF-1) is a CXC chemokine that activates and directs the migration of leukocytes that have CXCR4, which is the unique receptor for SDF-1. Although SDF-1/CXCR4 interaction has been implicated in various inflammatory conditions, its role in modulating coagulation has not been determined. We studied the plasma SDF-1 levels in 90 patients with suspected disseminated intravascular coagulation (DIC) and we found that circulating SDF-1 was significantly increased in the overt DIC patients and was also increased in overt DIC patients who have a poor outcome. We then tested in vitro whether SDF-1 can affect the expression of monocyte tissue factor (TF) and endothelial thrombomodulin (TM), and both of these play important roles in coagulopathy. SDF-1 did not affect the expression of surface TF protein and its function and the TF mRNA level in both monocytes and the monocytic leukemia cell line THP-1. SDF-1 also did not change the surface TM expression of endothelial cells. SDF-1 could enhance low-dose ADP induced platelet aggregation, although it failed by itself to induce aggregation. These findings suggest that plasma SDF-1 might be closely associated with hypercoagulability though its action as a platelet activator.
Kim
Hyun Kyung
HK
Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea.
Kim
Ji-Eun
JE
Chung
Junho
J
Lee
Dong Soon
DS
Han
Kyou-Sup
KS
Park
Seonyang
S
Cho
Han-Ik
HI
D002478:Cells, Cultured; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000097:blood* / Q000494:pharmacology; D004211:Disseminated Intravascular Coagulation / Q000097:blood*; D042783:Endothelial Cells / Q000378:metabolism; D006801:Humans; D009000:Monocytes / Q000378:metabolism; D010979:Platelet Function Tests; D011379:Prognosis; D018180:Thrombomodulin / Q000187:drug effects; D019851:Thrombophilia / Q000209:etiology*; D013925:Thromboplastin / Q000187:drug effects
C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D019743:Chemokines, CXC; D018180:Thrombomodulin; D013925:Thromboplastin