title: Neurospheres from human adipose tissue transplanted into cultured mouse embryos can contribute to craniofacial morphogenesis: a preliminary report.
abstract: Adipose-derived stromal cells (ASCs) are one of the most promising stem cell populations that differentiate into the mesodermal as well as neural lineages in vitro. In this study, we examined the neural differentiating potential of human ASCs by a neurosphere culture method. Neurospheres derived from human ASCs expressed Nestin and Musashi-1 genes, which are marker genes for neural stem cells. When these cells were labeled with green fluorescent protein gene transfection by Sendai virus vector and transplanted into the head region of mouse embryos using a whole embryo culture system, these cells were incorporated into the craniofacial development. Some transplanted cells appeared to migrate along the second branchial arches, implicating some similarity to the cranial neural crest cells. Although preliminary, our results support an idea that ASC-derived neurospheres have properties of neural progenitors in vitro and in vivo.
authors.affiliation: Clinical Research Center, National Hospital Organization Murayama Medical Center, Tokyo, Japan. tnagase@fb3.so-net.ne.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Nagase
authors.forename: Daisuke
authors.initials: D
authors.lastname: Matsumoto
authors.forename: Miki
authors.initials: M
authors.lastname: Nagase
authors.forename: Kotaro
authors.initials: K
authors.lastname: Yoshimura
authors.forename: Tomokuni
authors.initials: T
authors.lastname: Shigeura
authors.forename: Makoto
authors.initials: M
authors.lastname: Inoue
authors.forename: Mamoru
authors.initials: M
authors.lastname: Hasegawa
authors.forename: Masaaki
authors.initials: M
authors.lastname: Yamagishi
authors.forename: Masafumi
authors.initials: M
authors.lastname: Machida
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000637:transplantation; D000818:Animals; D002417:Cattle; D004622:Embryo, Mammalian / Q000601:surgery*; D005147:Facial Bones / Q000196:embryology; D005819:Genetic Markers; D006801:Humans; D007381:Intermediate Filament Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D009024:Morphogenesis / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D064231:Nestin; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D012886:Skull / Q000196:embryology; D018874:Spheroids, Cellular / Q000378:metabolism / Q000637:transplantation; D017154:Stromal Cells / Q000637:transplantation*
chemical_list: D005819:Genetic Markers; D007381:Intermediate Filament Proteins; C491297:MSI1 protein, human; C577484:NES protein, human; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D016601:RNA-Binding Proteins