A new method for ABO genotyping to avoid discrepancy between genetic and serological determinations.
In Japan and elsewhere, ABO genotyping is frequently used in forensic practice for identification of a decomposed body. However, the phenotype deduced from the genotyping data is occasionally inconsistent with the real phenotype. In this paper, we report a simple ABO genotyping method in which five single nucleotide polymorphism at nps 220, 261, 796, 802, and 803 are analyzed simultaneously to avoid discrepancies between genetic and serological determinations in ABO*A204, *O303, *O207, *cis-AB01 and *cis-AB02 alleles. This method can be used for the genotyping of badly decomposed remains or old bloodstains.
Shintani-Ishida
Kaori
K
Department of Forensic Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo, 113-0033, Japan. kaori@m.u-tokyo.ac.jp
Zhu
Bao-Li
BL
Maeda
Hitoshi
H
Uemura
Koichi
K
Yoshida
Ken-Ichi
K
D000017:ABO Blood-Group System / Q000235:genetics*; D000483:Alleles; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
D000017:ABO Blood-Group System; D017931:DNA Primers