Functional identification of cytokinesis-related genes from tobacco BY-2 cells.
The molecular mechanisms controlling cytokinesis in plant cell division cycle remains largely unknown. In this study, a functional approach was taken to identify genes that may play roles in cytokinesis in tobacco BY-2 cells, using fission yeast as the host organism. A total of 22 BY-2 genes that perturbed the terminal stage of cell division when ectopically expressed in yeast cells were isolated, among which, several encode for uncharacterized genes. Additionally, RT-PCR analysis indicated that four of the isolated genes were expressed in a cell cycle-dependent manner. Our results demonstrate that fission yeast system can be efficiently used to identify the genes that may function, either positively or negatively, in the regulation of cytokinesis. More importantly, the candidate genes we have isolated in this work can provide useful information for unraveling the regulators controlling cell separation at the late stage of BY-2 cell division.
Yu
Yi
Y
National Center for Plant Gene Research, National Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Wang
Hai-Yun
HY
Liu
Li-Na
LN
Chen
Zhi-Ling
ZL
Xia
Gui-Xian
GX
D002453:Cell Cycle; D002460:Cell Line; D048749:Cytokinesis / Q000235:genetics*; D005786:Gene Expression Regulation; D010940:Plant Proteins / Q000235:genetics*; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000166:cytology* / Q000235:genetics*
D010940:Plant Proteins