title: [Preparation of mitoxantrone liposomes and study on its in vivo distribution in rats after transdermal delivery].
abstract: OBJECTIVE
  To investigate in vivo distribution of the mitoxantrone(MIT) liposomes in SD rats after transdermal delivery.
  
  
  METHODS
  The mitoxantrone liposomes were prepared by thin-film method. Particle size, particle distribution and delta-potential of colloid solution were obtained on laser scatterometer; the encapsulation efficiency was measured by membrane diffusion technique. The time courses of mitoxantrone concentration in vivo after the transdermal delivery of mitoxantrone liposomes were measured by HPLC assays and compared with the injection of mitoxantrone solution.
  
  
  RESULTS
  The mean diameter of the MIT liposomes was 50.98 nm, with the entrapping efficiency of 100%. The liposomes had perfect shape. A significantly higher amount of mitoxantrone was delivered in cutis after transdermal delivery of mitoxantrone liposomes, compared with the injection of mitoxantrone solution; meanwhile, a significantly lower amount of mitoxantrone was delivered in plasma and other tissues after transdermal delivery of mitoxantrone liposomes, compared with the injection of mitoxantrone solution.
  
  
  CONCLUSIONS
  Transdermal delivery of mitoxantrone liposomes could be a potential therapy for cutaneous malignant melanoma.
authors.affiliation: Department of Pharmaceutics Sciences, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Chen
authors.forename: Shi-Xiang
authors.initials: SX
authors.lastname: Hou
authors.forename: Yi-Yi
authors.initials: YY
authors.lastname: Sun
authors.forename: Yu
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000627:therapeutic use; D005260:Female; D008081:Liposomes; D008545:Melanoma / Q000188:drug therapy; D046529:Microscopy, Electron, Transmission; D008942:Mitoxantrone / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000627:therapeutic use; D010316:Particle Size; D051381:Rats; D017207:Rats, Sprague-Dawley; D012878:Skin Neoplasms / Q000188:drug therapy
chemical_list: D000970:Antineoplastic Agents; D008081:Liposomes; D008942:Mitoxantrone