title: Heat-shock proteins modulate the incidence of apoptosis and oxidative stress in preimplantation mouse embryos.
abstract: We investigated the effect of antibodies to heat-shock proteins 60 and 70 on development, reactive oxygen species production, and incidence of apoptosis in preimplantation mouse embryos. Supplementation of culture media with antibodies to heat-shock proteins 60 and 70 resulted in significantly lower blastocyst development, hatching rate, reduced total cell number, and trophectoderm cell number, and increased the incidence of apoptosis in blastocysts compared to the control group.
authors.affiliation: Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Department of Obstetrics Gynecology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA.
authors.forename: Navid
authors.initials: N
authors.lastname: Esfandiari
authors.forename: Tommaso
authors.initials: T
authors.lastname: Falcone
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Goldberg
authors.forename: Ashok
authors.initials: A
authors.lastname: Agarwal
authors.forename: Rakesh K
authors.initials: RK
authors.lastname: Sharma
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001755:Blastocyst / Q000166:cytology* / Q000378:metabolism / Q000502:physiology*; D046149:Embryo Culture Techniques / Q000379:methods; D047108:Embryonic Development / Q000502:physiology; D005260:Female; D006360:Heat-Shock Proteins / Q000502:physiology*; D051379:Mice; D018384:Oxidative Stress / Q000502:physiology*; D011247:Pregnancy; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D006360:Heat-Shock Proteins; D017382:Reactive Oxygen Species