Polyploidy in mouse embryos derived from in vivo and in vitro fertilization is dependent on the timing of pregnant mare serum gonadotropin (PMSG) injection.
Nine hundred eighty-seven mouse embryos were cytogenetically analyzed at the two-cell stage to compare aneuploidy rates in embryos derived from both in vivo and in vitro fertilization using two different ovarian stimulation protocols. The rate of polyploidy was significantly (P<.001) elevated in both in vivo fertilized (15.6%) and in vitro fertilized (5.9%) embryos when PMSG injection was given in the afternoon instead of the morning (2.1% and 4.0% respectively).
Carrell
Douglas T
DT
Department of Surgery (Urology), University of Utah School of Medicine, Salt Lake City, Utah, USA. douglas.carrell@hsc.utah.edu <douglas.carrell@hsc.utah.edu>
Liu
Lihua
L
Christensen
Greg
G
D000818:Animals; D004622:Embryo, Mammalian / Q000187:drug effects / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro; D006064:Gonadotropins, Equine / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D023421:Models, Animal; D011123:Polyploidy; D011247:Pregnancy
D006064:Gonadotropins, Equine