[Optimization of cultivation conditions of Penicillium palitans strain 266 which synthesizes alpha-L-rhamnosidase].
The influence of different factors on the biosynthesis of extracellular alpha-L-rhamnosidase in Penicillium palitans at submerged cultivation has been investigated. It was shown that rhamnose and NaNO3 are the optimum carbon and nitrogen sources, respectively. The temperature of 26 degrees C, pH 5.0, growing in 50 ml of the medium at swing velocity 160 rev/min, adding of 5% of inoculum with cultivation for 4 days are the optimal parameters.
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D001431:Bacteriological Techniques; D002244:Carbon / Q000737:chemistry; D003470:Culture Media; D006026:Glycoside Hydrolases / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D009566:Nitrates / Q000737:chemistry; D010407:Penicillium / Q000201:enzymology* / Q000254:growth & development*; D013696:Temperature
D003470:Culture Media; D009566:Nitrates; D002244:Carbon; C031618:sodium nitrate; D006026:Glycoside Hydrolases; C022525:alpha-L-rhamnosidase