Pedobacter panaciterrae sp. nov., isolated from soil in South Korea.
A Gram-negative, facultatively anaerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated Gsoil 042T, was isolated from a soil sample of a ginseng field in Pocheon province (South Korea) and was characterized taxonomically by using a polyphasic approach. The isolate was positive for catalase and oxidase, but negative for gelatinase and production of indole and H2S. The predominant isoprenoid quinone was menaquinone 7 (MK-7). The major cellular fatty acids were summed feature 4 (C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH; 35.7 %), iso-C(15 : 0) (24.3 %) and iso-C(17 : 0) 3-OH (11.6 %). Comparative 16S rRNA gene sequence analysis showed that strain Gsoil 042T fell within the radiation of the cluster comprising Pedobacter species. On the basis of 16S rRNA gene sequence similarities, the phylogenetically closest recognized species of the genus Pedobacter to strain Gsoil 042T included Pedobacter heparinus DSM 2366T (98.1 %), Pedobacter africanus DSM 12126(T) (97.8 %) and Pedobacter caeni LMG 22862T (97.2 %). The 16S rRNA gene sequence similarity with other recognized species of the genus Pedobacter was less than 96.1 %. DNA-DNA hybridization experiments showed that the DNA-DNA relatedness between strain Gsoil 042T and its phylogenetically closest neighbours was less than 43 %. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 042T should be classified as representing a novel species in the genus Pedobacter, for which the name Pedobacter panaciterrae sp. nov. is proposed. The type strain is Gsoil 042T (=KCTC 12594T=LMG 23400T).
Yoon
Min-Ho
MH
Department of Bioenvironmental Chemistry, College of Agriculture and Life Sciences, Chungnam National University, Gung-dong 22, Yusung-gu, Daejeon 305-764, Republic of Korea.
Ten
Leonid N
LN
Department of Biology & Medicinal Science, Pai Chai University, Daejon 302-735, Republic of Korea.|Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Im
Wan-Taek
WT
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Lee
Sung-Taik
ST
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
D000693:Anaerobiosis; D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D050260:Carbohydrate Metabolism; D002374:Catalase / Q000032:analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry; D018093:Gelatinases / Q000032:analysis; D020459:Genes, rRNA; D006862:Hydrogen Sulfide / Q000378:metabolism; D007211:Indoles / Q000378:metabolism; D007723:Korea; D008969:Molecular Sequence Data; D009068:Movement; D009693:Nucleic Acid Hybridization; D010088:Oxidoreductases / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis / Q000737:chemistry; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D007211:Indoles; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C030374:indole; D010088:Oxidoreductases; D002374:Catalase; D018093:Gelatinases; D006862:Hydrogen Sulfide