The effect of betamethasone and IFN-gamma on replication of Toxoplasma gondii (RH strain) and nitric oxide production in Hela cell culture.
Toxoplasmosis is a protozoal infection caused by Toxoplasma gondii. Toxoplasmosis produce severe damage in patients who are immunosuppressed. In those who are immunosuppressed, latent infection can be reactivated resulting in acute disseminating disease. Betamethasone is a synthetic glycocorticoid, used as an anti-inflammatory and immunosuppressant in a wide variety of disorders. The aim of this study was evaluation of betamethasone as an immunosuppressor drug on infected cells by Toxoplasma gondii. In this study, at first HeLa cells were grown in 24 well culture plates in culture medium . When confluent monolayer was obtained, we compared 6 groups to evaluate the effect of betamethasone as a corticosteroid drug (two concentrations 4 and 40 micro g/ml) and the effect of IFN-gamma (100 IU/ml ) on growth, replication and Nitric Oxide (NO) production. The results showed, that high number of plaques were seen in group with 40 mug/ml of betamethasone and the lowest number of plaques were seen in group with 100 IU of IFN-gamma. The difference between plaque number in control and groups treated with IFN-gamma and betamethasone was significant (P<0.05). The groups with betamethasone or IFN-gamma without tachyzoites did not show any effect on cell structures. Replication rates in the wells treated with IFN-gamma were decreased significantly 72 h post inoculation in comparison with control group (P<0.05). There was no significant difference among different groups in NO production. The results indicated that betamethasone increase the invasion of tachyzoites to host cells in vitro.
Ghaffarifar
Fatemeh
F
Department of Parasitology, Faculty of Medicine, Tarbiat Modares University of Medical Sciences, Tehran, Iran. ghaffarifar@yahoo.com
Asl
Abdolhossein Dalimi
AD
Sharifi
Zohreh
Z
Ghasemi
Sakineh
S
Solhjoo
Kavos
K
Mohammadi
Shahla Roodbar
SR
D000818:Animals; D001623:Betamethasone / Q000494:pharmacology*; D005938:Glucocorticoids / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D007371:Interferon-gamma / Q000502:physiology*; D009569:Nitric Oxide / Q000096:biosynthesis*; D014122:Toxoplasma / Q000187:drug effects* / Q000378:metabolism
D005938:Glucocorticoids; D009569:Nitric Oxide; D007371:Interferon-gamma; D001623:Betamethasone