title: Prognostic significance of vascularity in cutaneous melanoma: pilot study using in vivo confocal scanning laser microscopy.
abstract: BACKGROUND
  Tumor vascularity may be of strong prognostic significance in cutaneous melanoma. We are the first to use a novel, noninvasive, in vivo confocal scanning laser microscope (CSLM) to evaluate vascularity in cutaneous melanoma.
  
  
  OBJECTIVE
  Our purpose was to apply a CSLM to assess vascularity in melanoma and to evaluate the prognostic significance of these findings.
  
  
  METHODS
  Patients with a suspicious pigmented lesion were prospectively recruited to undergo CSLM prior to skin biopsy, and those diagnosed with melanoma were included in this study. A blinded observer graded tumor vascularity from still digital CSLM images. The CSLM vascularity grading was correlated to tumor thickness and ulceration as a proxy for clinical prognosis.
  
  
  RESULTS
  Sixty-six patients and 67 lesions underwent imaging with CSLM. Eleven patients were diagnosed with melanoma, including six in situ and five invasive melanomas. Prominent vascularity was observed in all advanced melanomas. There was an overall increase in mean tumor thickness between the absent (x = 0.315 mm) to prominent (x = 1.51 mm) categories.
  
  
  CONCLUSIONS
  In this pilot study, vascularity was readily detected in cutaneous melanomas using CSLM. Prominent vascularity was observed in patients with advanced cutaneous melanomas. Our preliminary results are encouraging and indicate potential for the use of CSLM to assess vascularity in cutaneous melanoma, with potential prognostic and therapeutic implications.
authors.affiliation: Division of Anatomical Pathology, Department of Medicine, Dalhousie University, Halifax, NS.
authors.forename: Shannon
authors.initials: S
authors.lastname: Humphrey
authors.forename: Noreen M
authors.initials: NM
authors.lastname: Walsh
authors.forename: Laura
authors.initials: L
authors.lastname: Delaney
authors.forename: Iva
authors.initials: I
authors.lastname: Propperova
authors.forename: Richard G B
authors.initials: RG
authors.lastname: Langley
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D007834:Lasers; D008297:Male; D008545:Melanoma / Q000098:blood supply* / Q000473:pathology; D018613:Microscopy, Confocal; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D010865:Pilot Projects; D011379:Prognosis; D011446:Prospective Studies; D012878:Skin Neoplasms / Q000098:blood supply* / Q000473:pathology