title: Structure-activity relationship studies on quorum sensing ComX(RO-E-2) pheromone.
abstract: The ComX pheromone is a posttranslationally modified oligopeptide that stimulates natural genetic competence in Bacillus subtilis. Various ComX(RO-E-2) analogs were synthesized and their biological activities were studied to investigate structure-activity relationships. These results showed that the minimal active unit was the tripeptide, [3-5]ComX(RO-E-2), and all residues except the modified tryptophan residue were replaceable by alanine without total loss of activity.
authors.affiliation: Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan. okada-org@mail.tains.tohoku.ac,jp
authors.forename: Masahiro
authors.initials: M
authors.lastname: Okada
authors.forename: Hisao
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: Isao
authors.initials: I
authors.lastname: Sato
authors.forename: Soo Jeong
authors.initials: SJ
authors.lastname: Cho
authors.forename: David
authors.initials: D
authors.lastname: Dubnau
authors.forename: Youji
authors.initials: Y
authors.lastname: Sakagami
mesh_terms: D000409:Alanine / Q000737:chemistry; D019943:Amino Acid Substitution; D001407:Bacillus / Q000235:genetics; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D007763:Lac Operon / Q000235:genetics; D013058:Mass Spectrometry; D053038:Quorum Sensing / Q000187:drug effects*; D013329:Structure-Activity Relationship; D014364:Tryptophan / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; C087040:ComX protein, Bacillus subtilis; D007202:Indicators and Reagents; D014364:Tryptophan; D000409:Alanine