Trypanosoma cruzi-triggered meningoencephalitis is a CCR1/CCR5-independent inflammatory process.
Encephalitis rarely occurs during acute Trypanosoma cruzi infection. However, the central nervous system (CNS) is the major site of infection reactivation in immunocompromised patients. We show that the acute T. cruzi-triggered CD8-enriched meningoencephalitis paralleled the in situ expression of CCL3/MIP-1alpha and CCL5/RANTES mRNA. The frequency of CCR5-bearing cells was increased among peripheral blood mononuclear cells (PBMC) of infected mice. Further, CCL5/RANTES-driven in vitro PBMC migration was partially abrogated by the CCR1/CCR5 antagonist Met-RANTES. However, Met-RANTES treatment of infected mice altered neither parasitism nor intensity and nature of the CNS inflammation, indicating that T. cruzi-elicited meningoencephalitis is a CCR1/CCR5 independent process.
Silva
Andréa Alice
AA
Laboratory of Autoimmunity and Immunoregulation, Institute Oswaldo Cruz, Fiocruz, Av. Brasil 4365, Rio de Janeiro, 21045900, Brazil.
Roffê
Ester
E
Santiago
Helton
H
Marino
Ana Paula
AP
Kroll-Palhares
Karina
K
Teixeira
Mauro Martins
MM
Gazzinelli
Ricardo Tostes
RT
Lannes-Vieira
Joseli
J
D000818:Animals; D016827:CD8 Antigens / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects; D018946:Chemokine CCL5 / Q000031:analogs & derivatives / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D015870:Gene Expression; D007150:Immunohistochemistry; D007249:Inflammation / Q000188:drug therapy / Q000209:etiology*; D008590:Meningoencephalitis / Q000150:complications / Q000188:drug therapy / Q000209:etiology* / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D054389:Receptors, CCR1; D019713:Receptors, CCR5 / Q000502:physiology*; D019707:Receptors, Chemokine / Q000502:physiology*; D014349:Trypanosoma cruzi / Q000472:pathogenicity*
D016827:CD8 Antigens; C515301:Ccr1 protein, mouse; D018946:Chemokine CCL5; D004791:Enzyme Inhibitors; C108335:RANTES, Met-; D054389:Receptors, CCR1; D019713:Receptors, CCR5; D019707:Receptors, Chemokine