Production of membrane proteins using cell-free expression systems.
Different overexpression systems are widely used in the laboratory to produce proteins in a reasonable amount for functional and structural studies. However, to optimize these systems without modifying the cellular functions of the living organism remains a challenging task. Cell-free expression systems have become a convenient method for the high-throughput expression of recombinant proteins, and great effort has been focused on generating high yields of proteins. Furthermore, these systems represent an attractive alternative for producing difficult-to-express proteins, such as membrane proteins. In this review, we highlight the recent improvements of these cell-free expression systems and their direct applications in the fields of membrane proteins production, protein therapy and modern proteomics.
Liguori
Lavinia
L
University Joseph Fourier, HumProTher Laboratory, GREPI, CHU-Grenoble, 38043 Grenoble, France. lliguori@ujf-grenoble.fr
Marques
Bruno
B
Villegas-Méndez
Ana
A
Rothe
Romy
R
Lenormand
Jean-Luc
JL
D000818:Animals; D002474:Cell-Free System; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis*; D040901:Proteomics / Q000379:methods; D011994:Recombinant Proteins / Q000096:biosynthesis
D008565:Membrane Proteins; D011994:Recombinant Proteins