Glycosylation-induced depolarization facilitates subthreshold membrane oscillation in injured primary sensory neurons.
Subthreshold membrane potential oscillations (SMPO) in the injured dorsal root ganglion (DRG) neurons are involved in the generation of spontaneous activity, which can directly evoke neuropathic pain. Nerve injury usually triggers the synthesis of large quantities of membrane protein in nerve injured DRG neurons. Membrane proteins are glycosylated by addition of sugars, especially negatively charged sialic acid residues, which may depolarize the resting membrane potential (Vm), open voltage-gated channels in injured neurons, and cause spontaneous activity. In the present study, we aimed to determine if increased negative charge on the cell surface, carried by the sialic acid residues, could contribute to the generation of SMPO in injured DRG neurons. Intracellular recording was performed in DRG neurons following chronic constrictive injury (CCI) of the sciatic nerve. Results indicated that both A- and C-type injured DRG neurons exhibited a higher incidence of SMPO and more depolarized Vm than those of the control neurons. Ca(2+), Mg(2+), Mn(2+), or poly-lysine, a positively charged organic compound, when topically applied to the DRG, not only reduced SMPO but also caused a rapid hyperpolarizing shift in Vm. Topical application of neuraminidase to selectively remove sialic acid residues on the extracellular membrane normalized the depolarized Vm and inhibited both spontaneous and evoked SMPO. However, application of Ca(2+), Mg(2+), Mn(2+) or neuraminidase had no effect on excitability and Vm in normal neurons. The results demonstrated that the increase in negatively charged sialic acid residues on the extracellular membrane of neuronal somata is a critical factor in the generation of SMPO and hyperexcitability in injured sensory neurons.
Li
Chen-Xu
CX
Department of Physiology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China.
Jing
Yan-Ling
YL
Xie
Yi-Kuan
YK
D000704:Analysis of Variance; D000818:Animals; D005727:Ganglia, Spinal / Q000166:cytology / Q000502:physiology*; D006031:Glycosylation; D008564:Membrane Potentials / Q000502:physiology*; D008565:Membrane Proteins / Q000378:metabolism; D019158:N-Acetylneuraminic Acid / Q000378:metabolism*; D009409:Nerve Crush / Q000009:adverse effects; D009410:Nerve Degeneration / Q000150:complications / Q000503:physiopathology*; D009475:Neurons, Afferent / Q000502:physiology*; D009991:Oscillometry; D010146:Pain / Q000209:etiology / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000293:injuries / Q000378:metabolism / Q000503:physiopathology
D008565:Membrane Proteins; D019158:N-Acetylneuraminic Acid