Induction of HIV-specific memory T-cell responses by topical DermaVir vaccine.
In vivo antigen expression by plasmid DNA could provide a potent and cost-effective vaccine platform if its immunogenicity were improved to induce antigen-specific memory T-cell responses. To study these immune responses, we compared naked DNA vaccine with topical DermaVir formulated with the same HIV-1 (Gag) DNA in the mouse model. Topical DermaVir induced HIV-specific effector memory CD8(+) T-cell responses, which were 2.4-fold higher than those elicited by intramuscular injection of naked DNA. DermaVir, but not naked DNA vaccination, induced robust HIV-specific central memory T-cells responses, which are likely to be more efficient in mediating protective immunity. DermaVir formulation combined with topical administration provides an improved immunogenicity of antigen-expressing DNA vaccines.
Calarota
Sandra A
SA
Research Institute for Genetic and Human Therapy (RIGHT), 4400 East-West Hwy, Suite 1126, Bethesda, MD 20814, USA.
Weiner
David B
DB
Lori
Franco
F
Lisziewicz
Julianna
J
D016915:AIDS Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology*; D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000517:prevention & control*; D000287:Administration, Topical; D000818:Animals; D015483:HIV Antibodies / Q000097:blood; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D007156:Immunologic Memory; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D014611:Vaccination / Q000379:methods
D016915:AIDS Vaccines; C501366:DermaVir; D015483:HIV Antibodies