title: NOx (nitrite/nitrate) in patients with pediatric nephrotic syndrome.
abstract: To better understand the role of nitric oxide (NO) in pediatric nephrotic syndrome, we measured nitrite/nitrate (NOx) [NO2- and NO3-] in serum obtained from patients with several pediatric kidney diseases and investigated the locations of endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS). NOx in serum showed significantly higher levels than those in healthy controls (mean +/- SE: 297 +/- 55.7 vs. 158 +/- 13.1 pmol/10 microl). There was no significant difference between six patients with frequent relapse and five patients with nonfrequent relapse. The studies with immunostaining of iNOS and nitrotyrosine were negative for glomerulus in patients with nephrotic syndrome. Those findings suggest that NOx might indirectly influence disease progression in nephrotic syndrome.
authors.affiliation: Department of Paediatrics, Tokyo Medical University, 6-7-1 Nishishinjuku, Shinjuku-ku, Tokyo, 160-0023, Japan. hisashi@tokyo-med.ac.jp
authors.forename: Hisashi
authors.initials: H
authors.lastname: Kawashima
authors.forename: Yasuyo
authors.initials: Y
authors.lastname: Kashiwagi
authors.forename: Chiako
authors.initials: C
authors.lastname: Watanabe
authors.forename: Satoshi
authors.initials: S
authors.lastname: Sato
authors.forename: Shigeo
authors.initials: S
authors.lastname: Nishimata
authors.forename: Kouji
authors.initials: K
authors.lastname: Takekuma
authors.forename: Akinori
authors.initials: A
authors.lastname: Hoshika
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Watanabe
mesh_terms: D000293:Adolescent; D015415:Biomarkers / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D007678:Kidney Glomerulus / Q000201:enzymology / Q000473:pathology; D008297:Male; D009404:Nephrotic Syndrome / Q000097:blood* / Q000473:pathology; D009566:Nitrates / Q000097:blood*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism*; D009573:Nitrites / Q000097:blood*; D012008:Recurrence
chemical_list: D015415:Biomarkers; D009566:Nitrates; D009573:Nitrites; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III