[A method to produce the animal model of diabetic cardiomyopathy].
OBJECTIVE
To study a simple and scientific method to produce the animal model of diabetic cardiomyopathy.


METHODS
Injecting the streptozotocin (STZ) liquid at the left iliac region only once in the model group, observing the general condition, the change of eating level, drinking level, urine level and weight, examining the level of creatine kinase ( CK- MB) , lactate dehydrogenase (LDH-1 ) , insulin, blood sugar and urine sugar. Observe the cardiac muscle ultrastructure, the large vessel tunica intima and electrocardiogram.


RESULTS
After injecting the STZ 3 weeks, the model rats appear morbid, the value of eating, drinking, urine are increased and weight decreased. The model rat blood sugar, urine sugar, CK and LDH levels are higher than control rat. The model rat insulin level is lower than control group. The model rat cardiac muscle ultrastructure accord with the diabetic cardiomyopathy' s, and the fibrous or atheromatous plaque do not been found at the large vessel tunica intima.


CONCLUSIONS
The SD model rat appear the diabetic cardiomyopathy condition by injected the STZ (55mg/kg) at the left iliac region only once in 3 weeks. It is a simple and scientific method to produce the animal model of diabetic cardiomyopathy.
Zhang
Chun-hong
CH
Division of Cardiovascular Physiology and Pharmacology, School of Medicine, Xi'an Jiaotong University, Xi'an 710061, China.
Zang
Wei-jin
WJ
Xu
Jing
J
Yu
Xiao-jiang
XJ
Lv
Jun
J
Jing
Ai-yu
AY
Chen
Li-na
LN
Hu
Hao
H
Sun
Qiang
Q
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D009202:Cardiomyopathies / Q000209:etiology* / Q000503:physiopathology; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D005260:Female; D007274:Injections, Intraperitoneal; D008297:Male; D009206:Myocardium / Q000201:enzymology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin / Q000008:administration & dosage
D001786:Blood Glucose; D013311:Streptozocin