title: Enteral vaccination of rats against Fasciola hepatica using recombinant cysteine proteinase (cathepsin L1).
abstract: Cysteine proteinases released by Fasciola hepatica play a key role in parasite feeding, migration through host tissues and in immune evasion. Hence, a recombinant cysteine proteinase (CPFhW) expressed as inclusion bodies in Escherichia coli was used for enteral vaccination of rats against fasciolosis. We managed to activate this proteinase and found it to have cathepsin L1-like substrate preference. Enteral vaccination of rats induced a 78-80% protection against challenge with fluke metacercariae (mc). The immunised rats showed clear immunological response. The challenge with mc caused a remarkable infiltration of eosinophils into the peritoneal cavity of both the vaccinated rats and challenge control rats. However, CD8+ and CD4+ lymphocytes appeared in significantly higher numbers in the peritoneal fluid of vaccinated rats than in controls.
authors.affiliation: Institute of Biotechnology and Antibiotics, Department of Bioengineering, Starościńska 5, 02-516 Warsaw, Poland. kesikm@iba.waw.pl
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Kesik
authors.forename: Luiza
authors.initials: L
authors.lastname: Jedlina-Panasiuk
authors.forename: Monika
authors.initials: M
authors.lastname: Kozak-Cieszczyk
authors.forename: Andrzej
authors.initials: A
authors.lastname: Płucienniczak
authors.forename: Halina
authors.initials: H
authors.lastname: Wedrychowicz
mesh_terms: D000284:Administration, Oral; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002403:Cathepsins / Q000235:genetics / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics*; D005210:Fasciola hepatica / Q000201:enzymology / Q000235:genetics / Q000276:immunology*; D005211:Fascioliasis / Q000276:immunology / Q000517:prevention & control*; D005260:Female; D002479:Inclusion Bodies; D008297:Male; D010529:Peritoneal Cavity; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D014611:Vaccination / Q000379:methods; D014614:Vaccines, Synthetic / Q000008:administration & dosage* / Q000276:immunology
chemical_list: D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D002403:Cathepsins; C509464:cathepsin L1, Fasciola hepatica