Liquid chromatographic-electrospray tandem mass spectrometric determination of bisoprolol in human plasma.
An analytical method for the determination of bisoprolol in human plasma has been developed based on liquid chromatography-tandem mass spectrometry (LC-MS/MS). The analyte and internal standard (IS) diphenhydramine were cleaned up by protein precipitation with acetonitrile, reconstituted in mobile phase and separated by reversed-phase high-performance liquid chromatography (HPLC) using methanol:10 mm ammonium acetate:formic acid (70:30:0.1 v/v/v) as mobile phase. Detection was carried out by multiple reaction monitoring (MRM) on an LC-MS/MS system and was completed within 2.5 min. The assay was linear over the range 0.5-100 ng/mL with a limit of quantitation (LOQ) of 0.5 ng/mL. The intra- and inter-day precision levels were within 5.54 and 9.95%, respectively, while the accuracy was in the range 89.4-113%. This method has been utilized in a pharmacokinetic study, where healthy volunteers were treated with an oral dose of 5 mg bisoprolol.
Liu
Mingyuan
M
Research Center for Drug Metabolism, College of Life Science, Jilin University, Changchun 130023, People's Republic of China.
Zhang
Dan
D
Sun
Yantong
Y
Wang
Yingwu
Y
Liu
Zeyuan
Z
Gu
Jingkai
J
D000319:Adrenergic beta-Antagonists / Q000097:blood* / Q000493:pharmacokinetics; D017298:Bisoprolol / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000319:Adrenergic beta-Antagonists; D017298:Bisoprolol