Single amino acid substitutions in puroindoline-b mutants influence lipid binding properties.
External reflectance Fourier transform infrared (ER-FTIR) spectroscopy and surface pressure measurements have been used to characterize the interaction of wild-type puroindoline-b (Pin-b) and two mutant forms featuring single residue substitutions-namely, Gly-46 to Ser-46 (Pin-bH) and Trp-44 to Arg-44 (Pin-bS)-with condensed-phase monolayers of zwitterionic (L-alpha-dipalmitoylphosphatidylcholine, DPPC) and anionic (L-alpha-dipalmitoylphosphatidyl-dl-glycerol, DPPG) phospholipids. The interaction with anionic DPPG monolayers, monitored by surface pressure isotherms, was influenced significantly by mutations in Pin-b (p < 0.05); wild-type Pin-b showed the highest surface pressure change of 10.6 +/- 1.0 mN m-1, followed by Pin-bH (7.9 +/- 1.6 mN m-1) and Pin-bS (6.3 +/- 1.0 mN m-1), and the surface pressure isotherm kinetics were also different in each case. Integrated Amide I peak areas from corresponding ER-FTIR spectra confirmed the differences in adsorption kinetics, but also showed that differences in adsorbed amount were less significant, suggesting that mutations influence the degree of penetration into DPPG films. All Pin-b types showed evidence of interaction with DPPC films, detected as changes in surface pressure (5.6 +/- 1.1 mN m-1); however, no protein peaks were detected in the ER-FTIR spectra, which indicated that the interaction was via penetration with limited adsorption at the lipid/water interface. The expression of Pin-b mutants is linked to wheat endosperm hardness; therefore, the data presented here suggest that the lipid binding properties may be pivotal within the mechanism for this quality trait. In addition, the data suggest antimicrobial activities of Pin-b mutants would be lower than those of the wild-type Pin-b, because of decreased selectivity toward anionic phospholipids.
Clifton
Luke A
LA
School of Chemistry, Food Biosciences and Pharmacy, University of Reading, PO Box 226, Whiteknights, Reading RG6 6AP, United Kingdom.
Lad
Mitaben D
MD
Green
Rebecca J
RJ
Frazier
Richard A
RA
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000378:metabolism*; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D008969:Molecular Sequence Data; D010715:Phosphatidylglycerols / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D017354:Point Mutation; D017550:Spectroscopy, Fourier Transform Infrared; D014908:Triticum / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D010715:Phosphatidylglycerols; D010940:Plant Proteins; C082942:puroindoline protein, Triticum aestivum; D015060:1,2-Dipalmitoylphosphatidylcholine; C030345:1,2-dipalmitoylphosphatidylglycerol