title: Molecular characterization of a new serotype of duck hepatitis virus.
abstract: Duck hepatitis strains 90D and 04G were determined to be antigenically unrelated to type 1 duck hepatitis virus (DHV-1) by in vitro cross-neutralization assay. The genome sequences of 90D and 04G revealed that both strains of the new serotype DHV (N-DHV) possessed a typical picornavirus genome organization apart from the unique possession of three in-tandem 2A genes present in DHV-1. The 2A1, 2A2, and 2A3 proteins represented an aphthovirus-like 2A protein, AIG1-like protein, and human parechovirus-like 2A protein, respectively. The N-DHV genome displayed unique features, compared to the DHV-1 genome. The 366 nt 3'UTR of N-DHV, the largest determined thus far among picornaviruses, was 52 nt longer than DHV-1. The pairwise percent identity of the nucleic acid and amino acid sequences at 1D region of N-DHV and DHV-1 were only 69.1-69.7 and 70.1-70.5%, respectively. Finally, phylogenetic and evolutionary analysis of N-DHV revealed that the N-DHV and DHV-1 belong to two different clusters of a novel genus in the Picornaviridae family.
authors.affiliation: Graduate Institute of Veterinary Medicine, College of Bioresource and Agriculture, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 106, Taiwan.
authors.forename: Chun-Hsien
authors.initials: CH
authors.lastname: Tseng
authors.forename: Hsiang-Jung
authors.initials: HJ
authors.lastname: Tsai
mesh_terms: D020121:5' Untranslated Regions; D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral; D001483:Base Sequence; D003429:Cross Reactions; D004279:DNA, Viral / Q000235:genetics; D004372:Ducks; D019143:Evolution, Molecular; D016679:Genome, Viral; D004371:Hepatitis Virus, Duck / Q000145:classification* / Q000235:genetics* / Q000276:immunology; D006524:Hepatitis, Viral, Animal / Q000821:virology; D006801:Humans; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D010850:Picornaviridae Infections / Q000662:veterinary / Q000821:virology; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D012703:Serotyping; D014764:Viral Proteins / Q000235:genetics
chemical_list: D020121:5' Untranslated Regions; D000956:Antigens, Viral; D004279:DNA, Viral; D012367:RNA, Viral; D014764:Viral Proteins