Effect of elevated dissolved carbon dioxide concentrations on growth of Corynebacterium glutamicum on D-glucose and L-lactate.
The effect of increased dissolved carbon dioxide concentrations on growth of Corynebacterium glutamicum was studied with continuous turbidostatic cultures. The carbon sources were either l-lactate or d-glucose. To increase the dissolved carbon dioxide concentration the carbon dioxide partial pressure of the inlet gas stream pCO2,IN was increased stepwise from 0.0003 bar (air) up to 0.79 bar, while the oxygen partial pressure of the inlet gas stream was kept constant at 0.21 bar. For each resulting carbon dioxide partial pressure pCO2 the maximum specific growth rate mu(max) was determined from the feed rate resulting from the turbidostatic control. On d-glucose and pCO2 up to 0.26 bar, mu(max) was mostly constant around 0.58 h(-1). Higher pCO2 led to a slight decrease of mu(max). On l-lactate mu(max) increased gradually with increasing carbon dioxide partial pressures from 0.37 h(-1) under aeration with air to a maximum value of 0.47 h(-1) at a pCO2 of 0.26 bar. At very high pCO2 (0.81 bar) mu(max) decreased down to 0.35 h(-1) independent of the carbon source.
Bäumchen
Carsten
C
Biochemical Engineering, RWTH Aachen University, D-52074 Aachen, Germany.
Knoll
Arnd
A
Husemann
Bernward
B
Seletzky
Juri
J
Maier
Bernd
B
Dietrich
Carsten
C
Amoabediny
Ghassem
G
Büchs
Jochen
J
D002245:Carbon Dioxide / Q000494:pharmacology*; D048230:Corynebacterium glutamicum / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D010313:Partial Pressure
D002245:Carbon Dioxide; D019344:Lactic Acid; D005947:Glucose