Translocation of alpha-synuclein expressed in Escherichia coli.
alpha-Synuclein is a major component of Lewy bodies in Parkinson's disease. Although no signal sequence is apparent, alpha-synuclein expressed in Escherichia coli is mostly located in the periplasm. The possibilities that alpha-synuclein translocated into the periplasm across the inner membrane by the SecA or the Tat targeting route identified in bacteria and that alpha-synuclein was released through MscL were excluded. The signal recognition particle-dependent pathway is involved in the translocation of alpha-synuclein. The C-terminal 99-to-140 portion of the alpha-synuclein molecule plays a signal-like role for its translocation into the periplasm, cooperating with the central 61-to-95 section. The N-terminal 1-to-60 region is not required for this translocation.
Ren
Guoping
G
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China.
Wang
Xi
X
Hao
Shufeng
S
Hu
Hongyu
H
Wang
Chih-Chen
CC
D018988:Bacterial Translocation / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D006801:Humans; D010446:Peptide Fragments / Q000378:metabolism; D010957:Plasmids; D021381:Protein Transport; D011994:Recombinant Proteins / Q000378:metabolism; D014357:Trypsin; D051844:alpha-Synuclein / Q000235:genetics / Q000378:metabolism*
D010446:Peptide Fragments; D011994:Recombinant Proteins; D051844:alpha-Synuclein; D014357:Trypsin