A new molecular genetic diagnosis of the Btk(xid) mice.
A new method of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was developed for genetic typing of a point mutation of the Bruton's tyrosine kinase (Btk) gene in CBA/N mice bearing an X-linked recessive immunodeficiency (xid). Since restriction site useful for RFLP analysis does not exist in the spontaneous mutant Btk(xid) locus, an artificial restriction site was introduced by PCR amplification with a modified primer. The five genotypes of the Btk locus (Btk(xid)/ Btk(xid), Btk(xid) /Btk+ and Btk+/Btk+ females and Btk(xid)/Btk(null) and Btk+/Btk(null) males) could be distinguished by three patterns clearly and easily. This PCR-RFLP analytic method will be useful as a tool in the production of congenic mice and mice with multiple immunodeficient genes.
Takagi
Yumie
Y
Japan SLC, Inc., 3-5-1 Aoihigashi, Hamamatsu, Shizuoka 433-8114, Japan.
Masui
Norio
N
Yasuda
Mami
M
Ono
Shiro
S
D000077329:Agammaglobulinaemia Tyrosine Kinase; D000818:Animals; D005838:Genotype; D051379:Mice; D008808:Mice, Inbred CBA; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D014960:X Chromosome; D053632:X-Linked Combined Immunodeficiency Diseases / Q000175:diagnosis*
D011505:Protein-Tyrosine Kinases; D000077329:Agammaglobulinaemia Tyrosine Kinase; C000625951:Btk protein, mouse