Sequence analysis of full-length cDNA of sex chromosome-linked novel gene 2d-2F9 in Gallus gallus.
We obtained two novel W chromosome-linked chick genes by the use of female-male subtraction macroarrays, one of which, 2d-2F9, (recorded as AB188527 in DDBJ) did not have sufficient length (776 bp) to reveal its real form or characteristics. Hence, we obtained full-length Z-linked and W-linked 2d-2F9 genes of 2596 bp and 2589 bp respectively by the oligo-capping and RACE methods. Sequence analysis of these genes not only revealed that there is a counterpart of the W-linked 2d-2F9 gene on the Z chromosome, but also that there is a low homologous area at 5'-UTR between the W- and Z-kinked genes. Using this information, we designed a set of primers to identify sex and to select clones having the Z and W-linked gene (named 2d-2F9-Z and 2d-2F9-W), and also prepared two sets of primers for RT-PCR. These genes were found to be expressed constitutively and ubiquitously from the early embryo to the hatched chick, and they were assigned to the AAA ATP-superfamily.
Koyama
Yoshiyuki
Y
Graduate School of Applied Life Sciences, Nihon University, Kameino, Fujisawa, Japan.
Yamada
Daisuke
D
Saito
Yuichi
Y
Sato
Takahiro
T
Miyai
Saori
S
Tasaki
Momoko
M
Kato
Jun
J
Kasumi
Takafumi
T
Seki
Taiichiro
T
Ariga
Toyohiko
T
Ogihara
Jun
J
Mizuno
Shigeki
S
D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000737:chemistry* / Q000235:genetics*; D005784:Gene Amplification; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012730:Sex Chromosomes / Q000235:genetics*; D019849:Sex Determination Processes
D017931:DNA Primers; D018076:DNA, Complementary; D000251:Adenosine Triphosphatases