title: Crystal structure of the multidrug efflux transporter AcrB at 3.1A resolution reveals the N-terminal region with conserved amino acids.
abstract: Crystal structures of the bacterial multidrug transporter AcrB in R32 and C2 space groups showing both symmetric and asymmetric trimeric assemblies, respectively, supplemented with biochemical investigations, have provided most of the structural basis for a molecular level understanding of the protein structure and mechanisms for substrate uptake and translocation carried out by this 114-kDa inner membrane protein. They suggest that AcrB captures ligands primarily from the periplasm. Substrates can also enter the inner cavity of the transporter from the cytoplasm, but the exact mechanism of this remains undefined. Analysis of the amino acid sequences of AcrB and its homologs revealed the presence of conserved residues at the N-terminus including two phenylalanines which may be exposed to the cytoplasm. Any potential role that these conserved residues may play in function has not been addressed by existing biochemical or structural studies. Since phenylalanine residues elsewhere in the protein have been implicated in ligand binding, we explored the structure of this N-terminal region to investigate structural determinants near the cytoplasmic opening that may mediate drug uptake. Our structure of AcrB in R32 space group reveals an N-terminus loop, reducing the diameter of the central opening to approximately 15 A as opposed to the previously reported value of approximately 30 A for crystal structures in this space group with disordered N-terminus. Recent structures of the AcrB in C2 space group have revealed a helical conformation of this N-terminus but have not discussed its possible implications. We present the crystal structure of AcrB that reveals the structure of the N-terminus containing the conserved residues. We hope that the structural information provides a structural basis for others to design further biochemical investigation of the role of this portion of AcrB in mediating cytoplasmic ligand discrimination and uptake.
authors.affiliation: Berkeley Structural Genomics Center, Physical Biosciences Division, Lawrence Berkeley National Laboratory, USA. ddas@lbl.gov
authors.forename: Debanu
authors.initials: D
authors.lastname: Das
authors.forename: Qian Steven
authors.initials: QS
authors.lastname: Xu
authors.forename: Jonas Y
authors.initials: JY
authors.lastname: Lee
authors.forename: Irina
authors.initials: I
authors.lastname: Ankoudinova
authors.forename: Candice
authors.initials: C
authors.lastname: Huang
authors.forename: Yun
authors.initials: Y
authors.lastname: Lou
authors.forename: Andy
authors.initials: A
authors.lastname: DeGiovanni
authors.forename: Rosalind
authors.initials: R
authors.lastname: Kim
authors.forename: Sung-Hou
authors.initials: SH
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry*; D001692:Biological Transport; D017124:Conserved Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D003600:Cytosol / Q000378:metabolism; D008969:Molecular Sequence Data; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000378:metabolism; D011487:Protein Conformation
chemical_list: D001426:Bacterial Proteins; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D004364:Pharmaceutical Preparations