[Fosinopril but not metoprolol attenuated the increased matrix metallo-proteinases 9 expression at mRNA and protein levels of human umbilical vein endothelial cells exposed to oscillatory flow].
OBJECTIVE
To investigate the effect of fosinopril and metoprolol on metalloproteinases 9 (MMP9) expression of human umbilical vein endothelial cells (HUVECs) stimulated by oscillatory flow.


METHODS
HUVECs were exposed to steady laminar flow or oscillatory flow, laminar flow or oscillatory flow plus various concentrations (1 x 10(-7) mol/L, 1 x 10(-5) mol/L) of fosinopril and metoprolol for 4 and 24 hours. MMP9 mRNA and protein expressions of HUVECs were determined by RT-PCR and Western blot, respectively.


RESULTS
MMP9 expression at mRNA and protein levels were significantly increased in HUVECs exposed to oscillatory flow than that to laminar flow and these could be down-regulated by coincubation with fosinopril (1 x 10(-7) mol/L, 1 x 10(-5) mol/L, P < 0.01, P < 0.05, respectively) but not by co-incubation with metoprolol.


CONCLUSIONS
Fosinopril can attenuate the increased MMP9 expression at mRNA and protein levels of HUVECs exposed to oscillatory flow.
Cheng
Wen-Li
WL
National Intergrative Medicine Center for Cardiovascular Diseases, China-Japan Friendship Hospital, Beijing 100029, China.
Ke
Yuan-Nan
YN
Wang
Yong
Y
Chen
Li
L
Fan
Shu-Ying
SY
D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism*; D004730:Endothelium, Vascular; D017328:Fosinopril / Q000494:pharmacology*; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D008790:Metoprolol / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D013314:Stress, Mechanical; D014471:Umbilical Veins / Q000166:cytology
D012333:RNA, Messenger; D020780:Matrix Metalloproteinase 9; D008790:Metoprolol; D017328:Fosinopril