title: Sequencing and analysis of chromosome 1 of Eimeria tenella reveals a unique segmental organization.
abstract: Eimeria tenella is an intracellular protozoan parasite that infects the intestinal tracts of domestic fowl and causes coccidiosis, a serious and sometimes lethal enteritis. Eimeria falls in the same phylum (Apicomplexa) as several human and animal parasites such as Cryptosporidium, Toxoplasma, and the malaria parasite, Plasmodium. Here we report the sequencing and analysis of the first chromosome of E. tenella, a chromosome believed to carry loci associated with drug resistance and known to differ between virulent and attenuated strains of the parasite. The chromosome--which appears to be representative of the genome--is gene-dense and rich in simple-sequence repeats, many of which appear to give rise to repetitive amino acid tracts in the predicted proteins. Most striking is the segmentation of the chromosome into repeat-rich regions peppered with transposon-like elements and telomere-like repeats, alternating with repeat-free regions. Predicted genes differ in character between the two types of segment, and the repeat-rich regions appear to be associated with strain-to-strain variation.
authors.affiliation: Malaysia Genome Institute, UKM-MTDC Smart Technology Centre, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor DE, Malaysia.
authors.forename: King-Hwa
authors.initials: KH
authors.lastname: Ling
authors.forename: Marie-Adele
authors.initials: MA
authors.lastname: Rajandream
authors.forename: Pierre
authors.initials: P
authors.lastname: Rivailler
authors.forename: Alasdair
authors.initials: A
authors.lastname: Ivens
authors.forename: Soon-Joo
authors.initials: SJ
authors.lastname: Yap
authors.forename: Alda M B N
authors.initials: AM
authors.lastname: Madeira
authors.forename: Karen
authors.initials: K
authors.lastname: Mungall
authors.forename: Karen
authors.initials: K
authors.lastname: Billington
authors.forename: Wai-Yan
authors.initials: WY
authors.lastname: Yee
authors.forename: Alan T
authors.initials: AT
authors.lastname: Bankier
authors.forename: Fionnadh
authors.initials: F
authors.lastname: Carroll
authors.forename: Alan M
authors.initials: AM
authors.lastname: Durham
authors.forename: Nicholas
authors.initials: N
authors.lastname: Peters
authors.forename: Shu-San
authors.initials: SS
authors.lastname: Loo
authors.forename: Mohd Noor Mat
authors.initials: MN
authors.lastname: Isa
authors.forename: Jeniffer
authors.initials: J
authors.lastname: Novaes
authors.forename: Michael
authors.initials: M
authors.lastname: Quail
authors.forename: Rozita
authors.initials: R
authors.lastname: Rosli
authors.forename: Mariana
authors.initials: M
authors.lastname: Nor Shamsudin
authors.forename: Tiago J P
authors.initials: TJ
authors.lastname: Sobreira
authors.forename: Adrian R
authors.initials: AR
authors.lastname: Tivey
authors.forename: Siew-Fun
authors.initials: SF
authors.lastname: Wai
authors.forename: Sarah
authors.initials: S
authors.lastname: White
authors.forename: Xikun
authors.initials: X
authors.lastname: Wu
authors.forename: Arnaud
authors.initials: A
authors.lastname: Kerhornou
authors.forename: Damer
authors.initials: D
authors.lastname: Blake
authors.forename: Rahmah
authors.initials: R
authors.lastname: Mohamed
authors.forename: Martin
authors.initials: M
authors.lastname: Shirley
authors.forename: Arthur
authors.initials: A
authors.lastname: Gruber
authors.forename: Matthew
authors.initials: M
authors.lastname: Berriman
authors.forename: Fiona
authors.initials: F
authors.lastname: Tomley
authors.forename: Paul H
authors.initials: PH
authors.lastname: Dear
authors.forename: Kiew-Lian
authors.initials: KL
authors.lastname: Wan
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D022004:Chromosome Structures / Q000235:genetics*; D019295:Computational Biology; D016786:Eimeria tenella / Q000235:genetics*; D017125:Genes, Protozoan / Q000235:genetics*; D018598:Minisatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA