Preimplantation diagnosis for immunodeficiencies.
Preimplantation genetic diagnosis (PGD) has become an established procedure for the detection of single gene disorders, and has recently been performed together with human leukocyte antigen (HLA) typing for couples with children affected by genetic disorders that require HLA-identical stem cell transplantation therapy. For these couples, PGD can ensure the birth of an unaffected child, and because HLA-matched stem cell transplantation improves or completely restores the immune system, this child may also serve as a potential stem cell donor for affected siblings. This paper presents the first cumulative experience (18 cycles) of PGD for detection of the following immunodeficiencies: Wiscott-Aldrich syndrome, X-linked hyper-IgM syndrome (HIGM), X-linked hypohidrotic ectodermal dysplasia with immune deficiency (HED-ID), ataxia telangiectasia and Omenn syndrome, resulting in the transfer of unaffected embryos in 13 cycles and the birth of seven unaffected children, with one healthy pregnancy ongoing. HLA-identical stem cells from some of these children have been used for transplantation therapy, resulting in the restoration of normal function in siblings with HIGM and HED-ID.
Verlinsky
Yury
Y
Reproductive Genetics Institute, 2825 North Halsted Street, Chicago, IL 60657, USA.
Rechitsky
Svetlana
S
Sharapova
Tatyana
T
Laziuk
Katya
K
Barsky
Irina
I
Verlinsky
Oleg
O
Tur-Kaspa
Ilan
I
Kuliev
Anver
A
D004622:Embryo, Mammalian / Q000276:immunology*; D005260:Female; D006680:HLA Antigens; D006650:Histocompatibility Testing / Q000379:methods; D006801:Humans; D007153:Immunologic Deficiency Syndromes / Q000175:diagnosis*; D011247:Pregnancy; D019836:Preimplantation Diagnosis
D006680:HLA Antigens