[Study on antibacterial activity of pheromonicin-SP].
OBJECTIVE
To evaluate the bactericidal activity of purified Ph-SP via in vitro/in vivo assays.


METHODS
The recombinant plasmid was transformed into E. coli TG1 cells to produce the fusion peptide, Pheromonicin-Streptococcus pneumoniae (Ph-SP), the peptide was purified by CM sepharose ion-exchange column. In vitro/in vivo bactericidal assays were performed to determine the bactericidal activity of Ph-SP against Streptococcus pneumoniae.


RESULTS
Data of in vitro/in vivo assessments demonstrated that Ph-SP had bactericidal effects against Streptococcus pneumoniae, which was approximately 200 times more than that of penicillin G, on a molar basis, and 50 times more than that of vancomycin.


CONCLUSIONS
The Ph-SP may be of value as a novel model of antibiotics against Streptococcus pneumoniae infection.
Qin
Yan
Y
Laboratory of Transplant Engineering and Immunology, West China Hospital, Sichuan University, Chengdu 610041, China.
Zhang
Jie
J
Dai
Ping
P
Sun
Liang
L
Qiu
Xiao-qing
XQ
D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D005818:Genetic Engineering; D008297:Male; D051379:Mice; D008826:Microbial Sensitivity Tests; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D013045:Species Specificity; D013296:Streptococcus pneumoniae / Q000187:drug effects* / Q000254:growth & development
D000900:Anti-Bacterial Agents; D010455:Peptides