[Effects of recombinant adenovirus encoding human apM1 gene on proliferation and nitric oxide synthase activity in human umbilical vein endothelial cells].
OBJECTIVE
To evaluate the effects of recombinant adenovirus encoding human apM1 gene on proliferation and nitric oxide synthase (NOS) activity in human umbilical vein endothelial cells (HUVECs).


METHODS
Protein expression of apM1 in cell culture supernatant of HUVECs transfected with human Ad-apM1 was detected by double antibody sandwich ELISA. The effect of human adiponectin on cell proliferation was assessed by MTT assay. The total NOS and iNOS expressions were measured by chromatometre.


RESULTS
Human adiponectin protein level and total NOS and eNOS expressions were significant increased and iNOS expression significantly reduced in culture supernatant of HUVECs infected with Ad-apM1 compared to that in control HUVECs. The recombinant adenovirus had no influence on HUVECs growth as determined by MTT assay.


CONCLUSIONS
Human Ad-apM1 can be effectively expressed in HUVECs and do not influence HUVECs growth. Increased total NOS and eNOS expressions and decreased iNOS expression in HUVECs transfected with Ad-apM1 gene suggest a potential role of Ad-apM1 gene transfer for the prevention and treatment of arteriosclerosis.
Li
Bing-Rong
BR
Department of Endocrinology, First Affiliated Hospital of Chongqing University of Medical Science, Chongqing 400016, China.
Zheng
Dan
D
Deng
Hua-Cong
HC
Liu
Jin-Bo
JB
Lan
Li-Zhen
LZ
Zheng
Hong-Ting
HT
D000256:Adenoviridae / Q000235:genetics*; D052242:Adiponectin / Q000235:genetics; D049109:Cell Proliferation; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology; D015870:Gene Expression; D018014:Gene Transfer Techniques; D006801:Humans; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D014471:Umbilical Veins / Q000166:cytology
C499918:ADIPOQ protein, human; D052242:Adiponectin; D019001:Nitric Oxide Synthase