title: Novel elastic material from collagen for tissue engineering.
abstract: Elastic collagen gel (e-gel) was prepared from salmon atelocollagen fibrillar gel reinforced by 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) mediated cross-linking (f-gel). The preparation consisted of a simple heat treatment of the f-gel at 80 degrees C, in which the f-gel drastically shrank and the collagen fibril structure was deformed. The e-gel obtained showed rubber-like elasticity; its stress-strain behavior little changed through repeated stretching. The elongation at the breaking point was approximately 230%. Furthermore, normal human osteoblasts showed good attachment and proliferation on the e-gel. These results suggest its potential to be utilized for the development of tissue engineering.
authors.affiliation: Division of Molecular Chemistry, Graduate School of Engineering, Hokkaido University, Kita-13, Nishi-8, Kita-ku, Sapporo, Hokkaido 060-8628, Japan. yunoki@cris.hokudai.ac.jp
authors.forename: Shunji
authors.initials: S
authors.lastname: Yunoki
authors.forename: Kazuo
authors.initials: K
authors.lastname: Mori
authors.forename: Takeshi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Nagai
authors.forename: Masanobu
authors.initials: M
authors.lastname: Munekata
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D004548:Elasticity; D024022:Fibrillar Collagens / Q000737:chemistry* / Q000648:ultrastructure*; D006244:Hardness; D006801:Humans; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D013314:Stress, Mechanical; D013718:Tensile Strength; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D024022:Fibrillar Collagens