Bioluminescent signal system: bioluminescence immunoassay of pathogenic organisms.
The Ca(2+)-regulated photoprotein obelin has been examined as a label for bioluminescence immunoassay of infective agents. The hepatitis B virus (HbsAg) and the bacteria Escherichia coli and Shigella sonnei lipopolysaccharide (LPS) were chosen as model antigens. Chemically synthesized obelin-corresponding antibody conjugates were used in a solid-phase microplate immunoassay. The sensitivities achieved by the assay were 0.25 ng/mL for S. sonnei LPS and 0.375 ng/mL for HbsAg. A novel, filter-based immunoassay to determine bacterial admixtures in the environment was proposed. The NanoCeram filters were effectively applied to 'trap' and pre-concentrate pathogens from samples under study for the purposes of further detection and measurement of the absorbed material by bioluminescence immunoassay.
Frank
L
L
Institute of Biophysics, Russian Academy of Sciences Siberian Branch, Krasnoyarsk, 660036, Russia. lfrank@yandex.ru
Markova
S
S
Remmel
N
N
Vysotski
E
E
Gitelson
I
I
D000942:Antigens, Bacterial / Q000302:isolation & purification; D004926:Escherichia coli / Q000276:immunology / Q000302:isolation & purification* / Q000472:pathogenicity; D006514:Hepatitis B Surface Antigens / Q000302:isolation & purification; D006515:Hepatitis B virus / Q000276:immunology / Q000302:isolation & purification* / Q000472:pathogenicity; D007118:Immunoassay / Q000379:methods*; D008070:Lipopolysaccharides / Q000302:isolation & purification; D049449:Luminescence; D012764:Shigella sonnei / Q000276:immunology / Q000302:isolation & purification* / Q000472:pathogenicity; D015398:Signal Transduction
D000942:Antigens, Bacterial; D006514:Hepatitis B Surface Antigens; D008070:Lipopolysaccharides