title: Surfactin from Bacillus subtilis displays anti-proliferative effect via apoptosis induction, cell cycle arrest and survival signaling suppression.
abstract: The effect of surfactin on the proliferation of LoVo cells, a human colon carcinoma cell line, was examined. Surfactin strongly blocked the proliferation of LoVo cells by inducing pro-apoptotic activity and arresting the cell cycle, according to several lines of evidence on DNA fragmentation, Annexin V staining, and altered levels of poly (ADP-ribose) polymerase, caspase-3, p21(WAF1/Cip1), p53, CDK2 and cyclin E. The anti-proliferative activity of surfactin was mediated by inhibiting extracellular-related protein kinase and phosphoinositide 3-kinase/Akt activation, as assessed by phosphorylation levels. Therefore, our data suggest that surfactin may have anti-cancer properties as a result of its ability to downregulate the cell cycle and suppress its survival.
authors.affiliation: Department of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
authors.forename: Seo-Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Joo Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Seol-Hee
authors.initials: SH
authors.lastname: Kim
authors.forename: Hyun Jin
authors.initials: HJ
authors.lastname: Bae
authors.forename: Hwaseon
authors.initials: H
authors.lastname: Yi
authors.forename: Sang Hong
authors.initials: SH
authors.lastname: Yoon
authors.forename: Bon Sung
authors.initials: BS
authors.lastname: Koo
authors.forename: Moosik
authors.initials: M
authors.lastname: Kwon
authors.forename: Jae Youl
authors.initials: JY
authors.lastname: Cho
authors.forename: Choong-Eun
authors.initials: CE
authors.lastname: Lee
authors.forename: Sungyoul
authors.initials: S
authors.lastname: Hong
mesh_terms: D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001412:Bacillus subtilis / Q000737:chemistry*; D053148:Caspase 3 / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D024161:Collagen Type XI / Q000378:metabolism; D053222:Fas Ligand Protein / Q000378:metabolism; D006801:Humans; D055666:Lipopeptides; D010456:Peptides, Cyclic / Q000302:isolation & purification / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D019014:fas Receptor / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; C499319:COL11A2 protein, human; D024161:Collagen Type XI; C491901:FAS protein, human; D053222:Fas Ligand Protein; D055666:Lipopeptides; D010456:Peptides, Cyclic; D019014:fas Receptor; C009365:surfactin peptide; D053148:Caspase 3