Advanced applications of counter-current chromatography in the isolation of anti-tuberculosis constituents from Dracaena angustifolia.
Through several steps of in vitro based bioassay-guided fractionation, three highly potent anti-mycobacterial constituents (1-2 microg/mL minimum inhibitory concentrations) were isolated from Dracaena angustifolia Roxb. (Dracaenaceae). Isolation was expedited due to the application of new techniques in counter-current chromatography (CCC). The first of these applications, gradient-array CCC, enables the expansion of the high-resolution window (sweet spot) by applying successive CCC separations in different solvent systems to a crude extract. Further fractionation was also performed using the recently designed 1L fast-centrifugal partition chromatography (FCPC) rotor with the solvent system hexane:methyl-tert butylether:acetonitrile (10:1:10). Results indicated that gradient-array CCC and high-capacity FCPC can facilitate drug discovery efforts from complex natural products, increase reproducibility of separation schemes, and provide more rigid dereplication of previously isolated bioactive compounds.
Case
Ryan J
RJ
Institute for Tuberculosis Research, University of Illinois at Chicago, Chicago, IL 60612, USA.
Wang
Yuehong
Y
Franzblau
Scott G
SG
Soejarto
D Doel
DD
Matainaho
Lohi
L
Piskaut
Pius
P
Pauli
Guido F
GF
D003377:Countercurrent Distribution / Q000379:methods*; D031409:Dracaena / Q000737:chemistry*; D015394:Molecular Structure; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000254:growth & development; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D015203:Reproducibility of Results
D010936:Plant Extracts