title: Intranasal immunization with phosphorylcholine induces antigen specific mucosal and systemic immune responses in mice.
abstract: Phosphorylcholine (PC) is a structural component of a wide variety of pathogens including Streptococcus pneumoniae and Haemophilus influenzae, and anti-PC immune responses are known to protect mice against invasive bacterial diseases. The present study tested the capability of PC as an intranasal plurispecific vaccine against upper airway infections. BALB/c mice immunized with intranasal PC-keyhole limpet hemocyanin (KLH) plus cholera toxin (CT) as a mucosal adjuvant showed increased PC-specific IgM in serum, IgA in nasal wash and saliva, and numbers of PC-specific nasal and splenic antibody producing cells. Enhanced production of IL-4 and IFN-gamma by CD4+ T cells indicated the participation of Th2- and Th1-type cells. Salivary IgA antibodies produced by intranasal immunization with PC-KLH plus CT reacted to most strains of S. pneumoniae and H. influenzae. Further we demonstrated that the clearance of S. pneumoniae and H. influenzae from the nasal tract was significantly enhanced by nasal immunization with PC-KLH and CT. Thus, intranasal vaccination to induce PC-specific immune responses might help to prevent upper airway infections caused by S. pneumoniae and H. influenzae.
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan. noritan@m3.kufm.kagoshima-u.ac.jp
authors.forename: Norimitsu
authors.initials: N
authors.lastname: Tanaka
authors.forename: Satoshi
authors.initials: S
authors.lastname: Fukuyama
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Fukuiwa
authors.forename: Masaki
authors.initials: M
authors.lastname: Kawabata
authors.forename: Yukari
authors.initials: Y
authors.lastname: Sagara
authors.forename: Hiro-o
authors.initials: HO
authors.lastname: Ito
authors.forename: Yoko
authors.initials: Y
authors.lastname: Miwa
authors.forename: Takahiro
authors.initials: T
authors.lastname: Nagatake
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kiyono
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Kurono
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D002772:Cholera Toxin / Q000276:immunology; D005260:Female; D006193:Haemophilus influenzae / Q000276:immunology*; D006433:Hemocyanins / Q000276:immunology; D018928:Immunity, Mucosal; D007114:Immunization; D007071:Immunoglobulin A, Secretory / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D010767:Phosphorylcholine / Q000008:administration & dosage / Q000276:immunology*; D013296:Streptococcus pneumoniae / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D007071:Immunoglobulin A, Secretory; D010767:Phosphorylcholine; D002772:Cholera Toxin; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin