Preparative isolation and purification of bioactive constituents from Aconitum coreanum by high-speed counter-current chromatography coupled with evaporative light scattering detection.
Preparative high-speed counter-current chromatography (HSCCC) coupled with evaporative light scattering detection (ELSD) was employed for the isolation and purification of alkaloids from the roots of Aconitum coreanum (Lèvl.) Rapaics. The two-phase solvent system used in HSCCC was n-hexane-ethyl acetate-methanol-0.2M HCl (1:3.5:2:4.5, v/v/v/v). Six alkaloids were obtained and yielded 10.4 mg of Guanfu base P, 9.2 mg of Guanfu base G, 9.5 mg of Guanfu base F, 8.9 mg of atisine, 11.9 mg of Guanfu base A and 25.7 mg of Guanfu base I from 2 g of crude extracts. The purity of these compounds was 96.9%, 95.7%, 91.5%, 98.9%, 95.8% and 95.5%, respectively, as determined by high-performance liquid chromatography (HPLC). Their chemical structures were identified by MS, (1)H NMR and (13)C NMR.
Tang
Qingfa
Q
Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, China.
Yang
Chunhua
C
Ye
Wencai
W
Liu
Jinghan
J
Zhao
Shouxun
S
D031841:Aconitum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003377:Countercurrent Distribution / Q000379:methods*; D006576:Heterocyclic Compounds, 4 or More Rings / Q000302:isolation & purification*; D015394:Molecular Structure; D028321:Plant Preparations / Q000302:isolation & purification*; D018517:Plant Roots / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D012542:Scattering, Radiation; D012997:Solvents
D006576:Heterocyclic Compounds, 4 or More Rings; D028321:Plant Preparations; D012997:Solvents; C040615:Guan-fu base A