The chromophore structural changes during the photocycle of phytochrome: a combined resonance Raman and quantum chemical approach.
Phytochromes are sensory photoreceptors that, upon light irradiation, can be transformed between an inactive and an active state. The conversion is initiated by the photoisomerization of the cofactor, a linear methine-bridged tetrapyrrole, followed by conformational relaxations of the chromophore and the protein matrix that finally leads to the formation of the signaling state. To elucidate the underlying molecular processes, resonance Raman spectroscopy combined with quantum chemical calculations constitutes a powerful approach since it allows determination of the chromophore structure in the various states of phytochrome. On the basis of these studies, a molecular model for the photoinduced reaction cycle is derived.
Mroginski
Maria Andrea
MA
Technische Universität Berlin, Institut für Chemie, Max-Volmer-Laboratorium für Biophysikalische Chemie, Sekr. PC 14, Strasse des 17, Juni 135, D-10623 Berlin, Germany. andrea.mroginski@tu-berlin.de
Murgida
Daniel H
DH
Hildebrandt
Peter
P
D000465:Algorithms; D006860:Hydrogen Bonding; D007536:Isomerism; D008027:Light; D008697:Methane / Q000031:analogs & derivatives; D015394:Molecular Structure; D010834:Phytochrome / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects*; D011789:Quantum Theory; D013059:Spectrum Analysis, Raman / Q000379:methods; D045725:Tetrapyrroles / Q000737:chemistry
D045725:Tetrapyrroles; D010834:Phytochrome; D008697:Methane