title: Comprehensive analysis of gene expression in testes producing haploid germ cells using DNA microarray analysis.
abstract: The comprehensive changes in testicular gene expression before and after haploid germ cell differentiation were examined using microarray analysis. Approximately 14,000 expressed sequence tag (EST) clones of Mouse FANTOM Array ver.1 were hybridized with probes generated from mRNA of adult and juvenile (17 days postpartum) testes before the onset of spermiogenesis. Of 1315 genes that exhibited reproducible changes in expression (p < 0.05), 46% exhibited an increase of twofold or more in adults compared to juveniles, and 22% a decrease of twofold or more. The analysis not only confirmed the reported haploid-specific expression of several known genes, but also provided new information on the differential expression of various other genes, including upregulated genes such as Allc and Skd3 and downregulated genes such as hbb b1, before or after the onset of spermiogenesis. Based on the fundamental difference in expression profiles, and molecular functions of the encoded products, the genes were classified into several groups: postmeiotically upregulated genes encoding various enzymes, structural and regulatory proteins, and chaperones, and downregulated genes encoding haemoglobins and oxidation/reduction-related proteins or the machinery associated with protein synthesis, such as ribosomal proteins.
authors.affiliation: Department of Science for Laboratory Animal Experimentation, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Ike
authors.forename: Keizo
authors.initials: K
authors.lastname: Tokuhiro
authors.forename: Mika
authors.initials: M
authors.lastname: Hirose
authors.forename: Masami
authors.initials: M
authors.lastname: Nozaki
authors.forename: Yoshitake
authors.initials: Y
authors.lastname: Nishimune
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D020869:Gene Expression Profiling; D006238:Haploidy; D008297:Male; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D013094:Spermatozoa / Q000378:metabolism*; D013737:Testis / Q000166:cytology / Q000378:metabolism*