Anti-formyl peptide antibodies.
Antibodies that selectively bind to N-formylmethionyl leucyl phenylalanine (fMLF, also known as fMLP) have been generated. These antibodies bound to fMLF with higher affinity than to non-formylated peptide MLF: the differences in the binding energies between fMLF and MLF were 1.4->2.1 kcal/mol.
Tanaka
Fujie
F
Department of Molecular Biology and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA. ftanaka@scripps.edu
Jones
Teresa
T
Kubitz
Diane
D
Lerner
Richard A
RA
D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D002417:Cattle; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D015195:Drug Design; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry; D007700:Kinetics; D008956:Models, Chemical; D008968:Molecular Conformation; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000737:chemistry* / Q000378:metabolism; D010455:Peptides / Q000737:chemistry*; D011485:Protein Binding; D044042:Receptors, Formyl Peptide / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry
D000906:Antibodies; D007074:Immunoglobulin G; D010455:Peptides; D044042:Receptors, Formyl Peptide; D012710:Serum Albumin, Bovine; D009240:N-Formylmethionine Leucyl-Phenylalanine