title: [Study on extraction process of polysaccharide from Sargassum fusiforme by enzymatic treatment].
abstract: OBJECTIVE
  To investigate the optimal extraction process of polysaccharides from S. fusiforme by enzymatic treatment.
  
  
  METHODS
  The optimum extraction conditions were obtained by the experiment with the orthogonal design. The content of polysaccharides of S. fusiforme was determined by spectraphotometry.
  
  
  RESULTS
  The amount of enzyme and temperature significantly affected total polysaccharides of S. fusiforme.
  
  
  CONCLUSIONS
  The optimum extraction conditions include the addition of 1. 2 x 10 (4) U x 100 g(-1) enzyme into water at pH 4. 5, and the subsequent treatment for 10 min while the temperature is maintained at 45 degrees C.
authors.affiliation: School of Medicine, Shaoxing University, Shaoxing 312000, China. huafang_zhang@hotmail.com
authors.forename: Hua-fang
authors.initials: HF
authors.lastname: Zhang
authors.forename: Jing-shun
authors.initials: JS
authors.lastname: Jin
authors.forename: Rong-mei
authors.initials: RM
authors.lastname: Tan
authors.forename: Cheng
authors.initials: C
authors.lastname: Shen
mesh_terms: D002480:Cellulase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D011134:Polysaccharides / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D044647:Sargassum / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods; D013696:Temperature
chemical_list: D011134:Polysaccharides; D002480:Cellulase