Heme oxygenase-1 and interleukin-11 are overexpressed in stress-induced premature senescence of human WI-38 fibroblasts induced by tert-butylhydroperoxide and ethanol.
Acute repeated exposures to subcytotoxic concentrations of tert-butylhydroperoxide and ethanol trigger premature senescence of human diploid fibroblasts. In the present work we found an increased mRNA and protein level of interleukin-11 and heme oxygenase-1 in premature senescence of WI-38 human diploid foetal lung fibroblasts induced by both tert-butylhydroperoxide and ethanol. We tested whether interleukin-11 and heme oxygenase-1 could protect against tert-butylhydroperoxide- or ethanol-induced premature senescence when stable overexpression was established using a retroviral vector-based transduction. No protective effect was found against the decrease of the proliferative potential, the increase of the proportion of senescence-associated ss-galactosidase positive cells and the increase of the mRNA levels of six senescence-associated genes.
Pascal
Thierry
T
Unit of Research on Cellular Biology (URBC), Department of Biology, University of Namur (FUNDP), Rue de Bruxelles, 5000, Namur, Belgium.
Debacq-Chainiaux
Florence
F
Boilan
Emmanuelle
E
Ninane
Noëlle
N
Raes
Martine
M
Toussaint
Olivier
O
D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D016922:Cellular Senescence / Q000187:drug effects* / Q000235:genetics; D004790:Enzyme Induction; D000431:Ethanol / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D017370:Interleukin-11 / Q000096:biosynthesis* / Q000235:genetics; D008168:Lung / Q000187:drug effects* / Q000196:embryology / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D014161:Transduction, Genetic; D015854:Up-Regulation; D001616:beta-Galactosidase / Q000378:metabolism; D020122:tert-Butylhydroperoxide / Q000494:pharmacology*
C508611:IL11 protein, human; D017370:Interleukin-11; D012333:RNA, Messenger; D000431:Ethanol; D020122:tert-Butylhydroperoxide; C496541:HMOX1 protein, human; D051547:Heme Oxygenase-1; D001616:beta-Galactosidase