title: Leukocyte-platelet aggregates and monocyte tissue factor expression in bovines implanted with ventricular assist devices.
abstract: Infection and thromboembolism remain significant complications associated with ventricular assist device (VAD) support, including the newer rotary VADs, limiting wider adoption of this promising technology. These complications persist in spite of extensive preclinical testing in large animal models. The amount of biocompatibility information collected during preclinical trials is limited due to a lack of available assays. We thus developed three flow cytometric assays to measure leukocyte-platelet aggregates and monocyte tissue factor expression and applied them to 26 bovines implanted with two types of rotary VADs. All animals displayed low levels of circulating aggregates and monocytes expressing tissue factor prior to device implant. The assay values significantly increased following VAD implant, then usually declined to a lower, yet significantly elevated versus baseline, level indicative of ongoing inflammation. The implementation of more robust biocompatibility assays for the evaluation of cardiovascular device performance and modification might ultimately contribute to the development of safer artificial organs.
authors.affiliation: Bioengineering Department, University of Pittsburgh, Pittsburgh, USA.
authors.forename: Trevor A
authors.initials: TA
authors.lastname: Snyder
authors.forename: Kenneth N
authors.initials: KN
authors.lastname: Litwak
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tsukui
authors.forename: Takehide
authors.initials: T
authors.lastname: Akimoto
authors.forename: Shin'Ichiro
authors.initials: S
authors.lastname: Kihara
authors.forename: Kenji
authors.initials: K
authors.lastname: Yamazaki
authors.forename: William R
authors.initials: WR
authors.lastname: Wagner
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002417:Cattle; D005434:Flow Cytometry; D006098:Granulocytes / Q000378:metabolism; D006353:Heart-Assist Devices; D009000:Monocytes / Q000378:metabolism*; D010974:Platelet Aggregation / Q000502:physiology*; D011184:Postoperative Period
chemical_list: D001672:Biocompatible Materials