Human immunodeficiency virus type-1 subtypes of infected patients in Espírito Santo, Brazil.
Genetic variability of human immunodeficiency virus type-1(HIV-1) is a potential threat for both diagnosis and treatment of HIV/AIDS, as well as the development of effective vaccines. Up to now, HIV subtypes circulating among HIV-positive patients in the state of Espírito Santo were not known. In the present study, blood samples from 100 therapy-naïve HIV-1 infected patients were collected and the HIV subtype was determined through the Heteroduplex Mobility Assay (HMA). Ninety-seven out of 100 studied samples were subtyped by HMA, 73 samples (75.2%) were from subtype B, 9 (9.3%) from subtype F, 3 (3.1%) from subtype C, 6 (6.2%) Benv/Fgag, and another 6 (6.2%) Fenv/Bgag, what suggests that recombinant viruses were present in the studied samples. Twenty-eight percent of the subtype B samples were represented by the Brazilian B" subtype, which were identified by RFLP with Fok I. Data presented here demonstrate that the epidemiological characteristics of the HIV epidemic in the state of Espírito Santo are similar to those from the other Southeastern states and helped to better understand the genetic polymorphism of HIV in Brazil.
Cabral
Valéria P
VP
Laboratório de Imunologia Celular e Molecular, Núcleo de Doenças Infecciosas, Universidade Federal do Espírito Santo, 29040-091 Vitória, ES, Brasil.
Cunha
Carla B
CB
Magalhaes
Eneida F L
EF
Pinto-Neto
Lauro F
LF
Couto-Fernandez
José Carlos
JC
Dietze
Reynaldo
R
Morgado
Mariza G
MG
Ribeiro-Rodrigues
Rodrigo
R
D000328:Adult; D001938:Brazil; D005260:Female; D015752:Genes, env / Q000235:genetics*; D015750:Genes, gag / Q000235:genetics*; D014644:Genetic Variation; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D020180:Heteroduplex Analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length