title: The lipoprotein lipase gene serine 447 stop variant influences hypertension-induced left ventricular hypertrophy and risk of coronary heart disease.
abstract: LVH [LV (left ventricular) hypertrophy] is an independent risk factor for CHD (coronary heart disease). During LVH, the preferred cardiac energy substrate switches from FAs (fatty acids) to glucose. LPL (lipoprotein lipase) is the key enzyme in triacylglycerol (triglyceride) hydrolysis and supplies FAs to the heart. To investigate whether substrate utilization influences cardiac growth and CHD risk, we examined the association between the functional LPL S447X (rs328) variant and hypertension-induced LV growth and CHD risk. LPL-X447 has been shown to be more hydrolytically efficient and would therefore release more free FAs than LPL-S477. In a cohort of 190 hypertensive subjects, LPL X447 was associated with a greater LV mass index [85.2 (1.7) in S/S compared with 91.1 (3.4) in S/X+X/X; P=0.01], but no such association was seen in normotensive controls (n=60). X447 allele frequency was higher in hypertensives with than those without LVH {0.14 [95% CI (confidence interval), 0.08-0.19] compared with 0.07 (95% CI, 0.05-0.10) respectively; odds ratio, 2.52 (95% CI, 1.17-5.40), P=0.02}. The association of LPL S447X with CHD risk was then examined in a prospective study of healthy middle-aged U.K. men (n=2716). In normotensive individuals, compared with S447 homozygotes, X447 carriers were protected from CHD risk [HR (hazard ratio), 0.48 (95% CI, 0.23-1.00); P=0.05], whereas, in the hypertensives, X447 carriers had increased risk [HR, 1.54 (95% CI, 1.13-2.09) for S/S (P=0.006) and 2.30 (95% CI, 1.53-3.45) for X447+ (P<0.0001)] and had a significant interaction with hypertension in CHD risk determination (P=0.007). In conclusion, hypertensive LPL X447 carriers have increased risk of LVH and CHD, suggesting that altered FA delivery constitutes a mechanism through which LVH and CHD are associated in hypertensive subjects.
authors.affiliation: Centre for Cardiovascular Genetics, British Heart Foundation Laboratories, The Rayne Building, Department of Medicine, Royal Free and University College Medical School, London, UK. p.talmud@ucl.ac.uk
authors.forename: Philippa J
authors.initials: PJ
authors.lastname: Talmud
authors.forename: David M
authors.initials: DM
authors.lastname: Flavell
authors.forename: Khaled
authors.initials: K
authors.lastname: Alfakih
authors.forename: Jackie A
authors.initials: JA
authors.lastname: Cooper
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Balmforth
authors.forename: Mohan
authors.initials: M
authors.lastname: Sivananthan
authors.forename: Hugh E
authors.initials: HE
authors.lastname: Montgomery
authors.forename: Alistair S
authors.initials: AS
authors.lastname: Hall
authors.forename: Steve E
authors.initials: SE
authors.lastname: Humphries
mesh_terms: D000328:Adult; D001053:Apolipoproteins / Q000097:blood; D003327:Coronary Disease / Q000235:genetics* / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D006720:Homozygote; D006801:Humans; D006973:Hypertension / Q000150:complications / Q000235:genetics* / Q000378:metabolism; D017379:Hypertrophy, Left Ventricular / Q000235:genetics* / Q000378:metabolism; D008055:Lipids / Q000097:blood; D008071:Lipoprotein Lipase / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000378:metabolism; D011446:Prospective Studies; D012307:Risk Factors; D012694:Serine / Q000235:genetics*; D014280:Triglycerides / Q000097:blood
chemical_list: D001053:Apolipoproteins; D005227:Fatty Acids; D008055:Lipids; D014280:Triglycerides; D012694:Serine; D008071:Lipoprotein Lipase