title: [In vivo labeling of cells in the ependyma/subventricular zone of adult rats].
abstract: OBJECTIVE
  To identify the labeled cells in the ependyma/subventricular zone (SVZ) of normal adult rats by DiI injected into the lateral ventricle.
  
  
  METHODS
  Fifty male Sprague-Dawley rats were divided randomly into five groups (10 per group). All of the rats were injected with 10 microL of 2 g/L fluorescence dye DiI into the right lateral ventricle. The five groups of rats were sacrificed at 6 h, 12 h, 24 h, 36 h or 48 h after injection respectively. Hoechst 33258 staining was used to identify nuclei and laser confocal microscopy was used to detect the DiI-labeled cells and to measure the thickness of the tissue with DiI fluorescence in the wall of the left lateral ventricle.
  
  
  RESULTS
  After injection of the DiI into the right lateral ventricle, DiI- Hoechst 33258 double positive cells were found in the ependymal layer of the left lateral ventricular wall at 24 h and in the SVZ at 48 h as well. The thickness of the tissue with DiI fluorescence in the left ependyma/septal subventricular zone (SVZspt) and ependyma/postnatal equivalent of the ganglionic eminences (SVZge) remained unchanged at 12 h and 24 h after DiI injection. The thickness of the tissue with DiI fluorescence in the left ependyma/SVZge was significantly greater than that in the ependyma/SVZspt at all of the time points (P<0.05).
  
  
  CONCLUSIONS
  The ependyma/SVZ cells can be labeled by Dil 24-48 h after injection (10 microL of 2 g/L) into the lateral ventricle.
authors.affiliation: Environment and Genes Related to Diseases Key Laboratory of Education Ministry, Medical School of Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Peng-bo
authors.initials: PB
authors.lastname: Zhang
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Qian-yan
authors.initials: QY
authors.lastname: Kang
authors.forename: Ying-fang
authors.initials: YF
authors.lastname: Tian
authors.forename: Jian-jun
authors.initials: JJ
authors.lastname: Zhao
authors.forename: Qin-dong
authors.initials: QD
authors.lastname: Shi
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000473:pathology; D002232:Carbocyanines / Q000008:administration & dosage / Q000378:metabolism; D004805:Ependyma / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D007267:Injections; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling / Q000379:methods*; D013997:Time Factors
chemical_list: C035005:3,3'-dihexadecylindocarbocyanine; D002232:Carbocyanines