title: [Establishment of immortal lymphoblastoid cell bank of keloids pedigree].
abstract: OBJECTIVE
  To provide perpetual research materials for long term studies by establishing immortal lymphoblastoid cell bank of keloids pedigree.
  
  
  METHODS
  The immortal lymphoblastoid cell lines of keloids pedigree were established by Epstein-Barr virus transformation of peripheral blood B lymphocytes.
  
  
  RESULTS
  27 immortal lymphoblastoid cell lines of keloids pedigree were obtained successfully, all of the immortal lymphoblastoid cell lines were successfully revivificated after been frozen in liquid nitrogen.
  
  
  CONCLUSIONS
  It is important to establish immortal lymphoblastoid cell bank of keloids pedigree and provide long-term DNA materials for deep study of keloids in the future.
authors.affiliation: Department of Plastic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Song
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Gao
authors.forename: Xin
authors.initials: X
authors.lastname: Yan
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Liu
authors.forename: Yang
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001402:B-Lymphocytes; D018929:Cell Culture Techniques; D002461:Cell Line, Transformed; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007627:Keloid / Q000097:blood / Q000235:genetics*; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D014015:Tissue Banks; D055815:Young Adult