[Study on extraction process of polysaccharide from Sargassum fusiforme by enzymatic treatment].
OBJECTIVE
To investigate the optimal extraction process of polysaccharides from S. fusiforme by enzymatic treatment.


METHODS
The optimum extraction conditions were obtained by the experiment with the orthogonal design. The content of polysaccharides of S. fusiforme was determined by spectraphotometry.


RESULTS
The amount of enzyme and temperature significantly affected total polysaccharides of S. fusiforme.


CONCLUSIONS
The optimum extraction conditions include the addition of 1. 2 x 10 (4) U x 100 g(-1) enzyme into water at pH 4. 5, and the subsequent treatment for 10 min while the temperature is maintained at 45 degrees C.
Zhang
Hua-fang
HF
School of Medicine, Shaoxing University, Shaoxing 312000, China. huafang_zhang@hotmail.com
Jin
Jing-shun
JS
Tan
Rong-mei
RM
Shen
Cheng
C
D002480:Cellulase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D011134:Polysaccharides / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D044647:Sargassum / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods; D013696:Temperature
D011134:Polysaccharides; D002480:Cellulase