title: Expression of Aspergillus oryzae phytase gene in Aspergillus oryzae RIB40 niaD(-).
abstract: Aspergillus oryzae RIB40 niaD(-) was transformed using a plasmid constructed with the A. oryzae phytase gene and pNAN8142 vector. The culture broth of the transformant, which was grown in a medium containing starch as a carbon source and polyvinylpyrrolidone showed phytase activity of a maximum of 2.0 units ml(-1) at 37 degrees C, pH 5.5.
authors.affiliation: Department of Applied Chemistry and Biotechnology, Faculty of Engineering, University of Fukui, 9-1 Bunkyo 3-chome, Fukui-shi 910-8507, Japan. uchida@acbio.fukui-u.ac.jp
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Uchida
authors.forename: Shinya
authors.initials: S
authors.lastname: Arakida
authors.forename: Tatsuji
authors.initials: T
authors.lastname: Sakamoto
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Kawasaki
mesh_terms: D010832:6-Phytase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D001236:Aspergillus oryzae / Q000145:classification* / Q000502:physiology*; D004789:Enzyme Activation; D015870:Gene Expression / Q000502:physiology; D030821:Plants, Genetically Modified / Q000201:enzymology*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism; D013045:Species Specificity
chemical_list: D011994:Recombinant Proteins; D010832:6-Phytase