[Triterpenes from herb of Potentilla chinesis].
OBJECTIVE
To study the chemical constituents of Potentilla chinesis and their anticancer activities.


METHODS
Chemical constituents were isolated by repeated column chromatography (Toyopearl HW-40C and preparative HPLC). The structures were elucidated on the basis of spectral data analysis. The isolated compounds were screened with two anticancer models.


RESULTS
Fifteen triterpenes, alpha-amyrin (1) , beta-amyrin (2) , ursolic acid (3) , corosolic acid (4), euscaphic acid (5) , pomolic acid (6) , tormentic acid (7), 2alpha, 3alpha-dihydroxyurs-12-en-28-oic acid (8), 2beta, 3beta, 19alpha-trihydroxyurs-12-en-28-oic acid (9), asiatic acid (10) , 24-hydroxy tormentic acid (11) , myrianthic acid (12), oleanolic acid (13), maslinic acid (14) and 2alpha, 3alpha-dihydroxyolean-12-en-28-oic acid (15) , were isolated from P. chinesis.


CONCLUSIONS
Compounds 1, 2, 4 -15 were isolated from the plant for the first time. Compounds 4, 8 - 10, 12, 14 and 15 show anticancer activities. Compounds 4, 9 show strong activities.
Liu
Pu
P
College of Pharmaceuticals and Biotechnology, Tianjin University, Tianjin 300072, China.
Duan
Hong-quan
HQ
Pan
Qin
Q
Zhang
Yan-wen
YW
Yao
Zhi
Z
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry*; D031987:Potentilla / Q000737:chemistry*; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D009828:Oleanolic Acid / Q000031:analogs & derivatives
D000972:Antineoplastic Agents, Phytogenic; D014315:Triterpenes; C435248:2,3,19-trihydroxy-12-oleanen-28-oic acid; C113861:corosolic acid; C412811:maslinic acid; D009828:Oleanolic Acid