Slide enzyme-linked immunosorbent assay for the diagnosis of Babesia bigemina infection in bovines.
A slide enzyme-linked immunosorbent assay (SELISA) for the diagnosis of Babesia bigemina infection in cattle was standardized. Acetone-fixed whole Babesia bigemina-infected erythrocytes on micro-slides were immunoreacted with bovine serum samples followed by antibovine horseradish peroxidase conjugate and developed using diaminobenzidine tetrahydrochloride as a substrate. The positive immunoreactivity (staining pattern) was visualized in the form of dark brown piroplasms. Using the laboratory-standardized SELISA with a sensitivity of 94.4%, the seroprevalence of babesiosis was studied in cattle from two endemic areas of the disease. In comparison to IFAT, SELISA detected higher number of serum samples positive for bovine babesiosis.
Ravindran
R
R
Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, India, drreghuravi@yahoo.com.
Mishra
A K
AK
Rao
J R
JR
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D001403:Babesia / Q000302:isolation & purification*; D001404:Babesiosis / Q000097:blood / Q000469:parasitology / Q000662:veterinary*; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000175:diagnosis / Q000469:parasitology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards / Q000662:veterinary*; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D012015:Reference Standards; D012680:Sensitivity and Specificity
D000913:Antibodies, Protozoan