title: A new method of defense response analysis using a transient expression system in rice protoplasts.
abstract: We established a new plant defense response assay using a transient expression system in rice protoplasts. The assay system sensitively detected defense induction by flagellin, which had previously been assigned to a specific elicitor. Our assay system provides a rapid and efficient way to dissect rice defense mechanisms.
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, Japan.
authors.forename: Ryota
authors.initials: R
authors.lastname: Takai
authors.forename: Takashi
authors.initials: T
authors.lastname: Kaneda
authors.forename: Akira
authors.initials: A
authors.lastname: Isogai
authors.forename: Seiji
authors.initials: S
authors.lastname: Takayama
authors.forename: Fang-Sik
authors.initials: FS
authors.lastname: Che
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005408:Flagellin / Q000235:genetics / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D017930:Genes, Reporter / Q000235:genetics; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D012275:Oryza / Q000166:cytology / Q000502:physiology*; D010935:Plant Diseases; D011523:Protoplasts / Q000502:physiology*; D048790:Renilla / Q000737:chemistry
chemical_list: D005408:Flagellin; D008156:Luciferases