title: XPS investigation of DNA binding to zirconium-phosphonate surfaces.
abstract: The surface coverage of phosphorylated oligonucleotides immobilized on a zirconium-phosphonate surface was analyzed using X-ray photoelectron spectroscopy (XPS). By quantifying the intensity of the N 1s signal originating from the oligonucleotide and the Zr 3d peak from the metal-phosphonate surface, the surface coverage of the oligonucleotide could be calculated with a modified substrate-overlayer model. We found relatively low surface coverages indicating that once covalently bound via the terminal phosphate the polymer chain further physisorbs to the surface limiting the adsorption of additional molecules.
authors.affiliation: Department of Chemistry, University of Florida, Gainesville, FL 32611-7200, USA.
authors.forename: Sarah M
authors.initials: SM
authors.lastname: Lane
authors.forename: Julien
authors.initials: J
authors.lastname: Monot
authors.forename: Marc
authors.initials: M
authors.lastname: Petit
authors.forename: Bruno
authors.initials: B
authors.lastname: Bujoli
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Talham
mesh_terms: D004277:DNA, Single-Stranded / Q000737:chemistry*; D063065:Organophosphonates / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods*; D013499:Surface Properties; D014965:X-Rays
chemical_list: D004277:DNA, Single-Stranded; D063065:Organophosphonates