Somatic mutations of the beta-TrCP gene in gastric cancer.
Beta-TrCP is a component of the ubiquitin ligase complex targeting beta-catenin for proteasomal degradation, and is a negative regulator of Wnt/beta-catenin signaling. To determine whether genetic alterations of the beta-TrCP gene are involved in the development or progression of gastric cancer, we analyzed its somatic mutations in 95 gastric cancers by single-strand conformational polymorphism and sequencing. We found five missense mutations (5.3%): A99V, H342Y, H425Y, C206Y, and G260E. Tissue carrying mutations showed moderate to strong cytoplasmic and/or nuclear staining of beta-catenin by immunohistochemistry. Thus, somatic mutations of the beta-TrCP gene may contribute to the development of gastric cancer through beta-catenin stabilization.
Kim
Chang Jae
CJ
Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Song
Jae Hwi
JH
Cho
Yong Gu
YG
Kim
Young Sill
YS
Kim
Su Young
SY
Nam
Suk Woo
SW
Yoo
Nam Jin
NJ
Lee
Jung Young
JY
Park
Won Sang
WS
D001483:Base Sequence; D017931:DNA Primers; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D009154:Mutation; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D051176:beta Catenin / Q000378:metabolism; D044844:beta-Transducin Repeat-Containing Proteins / Q000235:genetics*
D017931:DNA Primers; D004273:DNA, Neoplasm; D051176:beta Catenin; D044844:beta-Transducin Repeat-Containing Proteins