title: PTA wastewater molecular toxicity detected with gene chip.
abstract: The purified terephthalic acid (PTA) petrochemical wastewater molecular toxicity detected by use of Mouse Genome 430A 2.0 GeneChip was conducted in this research. The toxic dose to male mice was 0.03 g/(kg x d) of PTA in the wastewater. The mice liver total RNA was isolated as the temple for synthesis of cDNA and then the cDNA as the temple for synthesis of cRNA. Hybridizing the cRNA with the target genes on the gene chip, there were 232 genes expression levels up-regulated and 74 genes down-regulated discovered obviously. The foremost 40 genes for both the highest and the lowest expression levels involved endogenetic steroid and hormone metabolism, immune system, the leukocyte activity and inflammation, detoxification in liver, reproduction and growth hormone, regulation immune factors of anti-tumor and anti-infection and cancer to the mice sampled. The data suggest the PTA wastewater contained over 5 aromatics and their toxicities integrated were much higher than the pure chemical PTA. And the pure chemical PTA toxicities data cannot be used to evaluate the toxicity of the PTA wastewater instead.
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, China.
authors.forename: Cheng-Jun
authors.initials: CJ
authors.lastname: Zhu
authors.forename: Shu-Pei
authors.initials: SP
authors.lastname: Cheng
authors.forename: Xu-Xiang
authors.initials: XX
authors.lastname: Zhang
authors.forename: You-Zhe
authors.initials: YZ
authors.lastname: Lang
authors.forename: Shi-Lei
authors.initials: SL
authors.lastname: Sun
authors.forename: Ji-Dong
authors.initials: JD
authors.lastname: Gu
authors.forename: Da-Yong
authors.initials: DY
authors.lastname: Zhao
authors.forename: Wen-Yang
authors.initials: WY
authors.lastname: Pan
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Yu
mesh_terms: D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000235:genetics; D008099:Liver / Q000737:chemistry; D008297:Male; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D010795:Phthalic Acids / Q000032:analysis / Q000633:toxicity*; D012313:RNA / Q000302:isolation & purification*; D018675:Toxicity Tests / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
chemical_list: D018076:DNA, Complementary; D010795:Phthalic Acids; D014874:Water Pollutants, Chemical; D012313:RNA; C011363:terephthalic acid