Microarray-based screening of jasmonate-responsive genes in Arabidopsis thaliana.
Jasmonates comprise a family of plant hormones that regulate gene expression to modulate diverse developmental and defensive processes. To screen a set of jasmonate-responsive Arabidopsis genes, we performed a microarray analysis using an Affymetrix GeneChip containing about 8,300 gene probes synthesized in situ. External treatment with 100 microM methyl jasmonate resulted in significant changes (more than twofold increases or decreases) in the expression levels of 137 genes in the rosette leaves of 5-week-old Arabidopsis plants. Of these, 74 genes were up-regulated, including those involved in jasmonate biosynthesis, defense responses, oxidative stress responses, senescence, and cell wall modification. In contrast, the expression of genes involved in chlorophyll constitution and photosynthesis was down-regulated. Most importantly, the jasmonate treatment significantly reduced transcripts of abscisic acid-responsive cold/drought-stress genes, which suggests that an antagonistic interaction occurs between the jasmonate and abscisic acid signaling pathways in abiotic stress responses. Northern blot analysis of some selected genes revealed that the jasmonate-responsive genes exhibited unique time-course expression patterns after the external jasmonate treatment. Based on the basic clustering of the genes, we established a likely regulation scenario: the genes induced early after treatment are involved in signaling mechanisms that activate or repress other genes, whereas intermediate- and late-accumulating genes are activated by the signaling mechanisms and are subsequently involved in the ultimate jasmonate-modulated cellular responses.
Jung
Choonkyun
C
School of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University, Seoul 151-921, Korea.
Lyou
Seoung Hyun
SH
Yeu
SongYion
S
Kim
Myeong Ae
MA
Rhee
Sangkee
S
Kim
Minkyun
M
Lee
Jong Seob
JS
Choi
Yang Do
YD
Cheong
Jong-Joo
JJ
D000085:Acetates / Q000494:pharmacology*; D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics*; D003517:Cyclopentanes / Q000494:pharmacology*; D005030:Ethylenes / Q000494:pharmacology; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D054883:Oxylipins; D015854:Up-Regulation / Q000187:drug effects
D000085:Acetates; D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D005030:Ethylenes; D054883:Oxylipins; C072239:methyl jasmonate; C036216:ethylene