title: Transition from long- to short-lived transient pores in giant vesicles in an aqueous medium.
abstract: We have observed large pores in the membrane of giant vesicles in an aqueous medium. The lifetime of the pores can reach 2 min and their size (a few micrometers) enables their visualization by fluorescence microscopy. These pores are obtained thanks to a destabilization of the membrane due to the synergistic action of a cone-shaped and nitrobenzodiazole (NBD) labeled phospholipid illuminated in the presence of dithionite. The opening of the pore occurs immediately after illumination starts so that it can be accurately triggered. A concomitant decrease of the vesicle radius is observed; we interpret it as a solubilization of the membrane. Depending on the rate of this solubilization, long- or short-lived pores were observed. At the transition between both regimes for a 30 microm vesicle, the solubilization rate was about 1/300 s{-1} . In order to interpret these observations, we have revisited the current model of pore opening to take into account this solubilization. This proposed model along with simulations enables us to prove that solubilization explains why the large long-lived pores are observed even in an aqueous medium. The model also predicts the solubilization rate at the transition between a single long-lived pore and a cascade of short-lived pores.
authors.affiliation: CNRS, UMR 7099, Paris, F-75005 France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Rodriguez
authors.forename: Sophie
authors.initials: S
authors.lastname: Cribier
authors.forename: Frédéric
authors.initials: F
authors.lastname: Pincet
mesh_terms: D003198:Computer Simulation; D007700:Kinetics; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D008560:Membrane Fluidity; D021962:Membrane Microdomains / Q000737:chemistry; D008956:Models, Chemical; D008958:Models, Molecular; D010743:Phospholipids / Q000737:chemistry*; D016062:Porosity; D012996:Solutions; D013997:Time Factors; D014867:Water / Q000737:chemistry*
chemical_list: D008051:Lipid Bilayers; D008081:Liposomes; D010743:Phospholipids; D012996:Solutions; D014867:Water