High-throughput genotyping of filamentous fungus Aspergillus oryzae based on colony direct polymerase chain reaction.
High-throughput genotyping of Aspergillus oryzae was achieved using an FTA card for the extraction of a genomic DNA template for polymerase chain reaction from a fungal colony growing on an agar plate. This method was then applied to detect other fungal species from agar slants and food materials. This method offers a convenient tool for the genotyping of filamentous fungi without using an organic solvent or specialized equipment.
Suzuki
Satoshi
S
National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan. satosuz@affrc.go.jp
Taketani
Hiroko
H
Kusumoto
Ken-Ichi
K
Kashiwagi
Yutaka
Y
D001236:Aspergillus oryzae / Q000235:genetics* / Q000302:isolation & purification*; D002874:Chromosome Mapping / Q000379:methods*; D004271:DNA, Fungal / Q000235:genetics*; D016681:Genome, Fungal / Q000235:genetics*; D005838:Genotype; D016133:Polymerase Chain Reaction / Q000379:methods*
D004271:DNA, Fungal