Magnetic counter-gravity flow separation of electrically prepolarised lymphoid cells.
A novel principle is proposed for a differential separation of live cells (such as leucocytes) from a main flow. A microfluidic device with planar insulated electrodes as the side walls of the channel was manufactured and tested. An array of insulated vertical conductor wires was inserted along the axis of the channel and used to impose Lorentz forces upon polarisable particles that moved with the flow. Polystyrene microspheres and lymphoid cell lines (DOHH2 and K562) were used to test the ability of the setting to impose a force field that induced consistent vertical motion. The direction of electric current was found to directly influence the number of cells or microspheres that were sampled at the surface of the flow. Lorentz force was considered to be active upon cells due to an overall polarisation of the membrane surface. The consequence of the magnetic force was that the polarised cells were moved vertically upwards (opposing gravity). The setting was effective for increasing the number of extracted cells from a main flow or for increasing the concentration of DOHH2 cells in a mixed population with K562 in culture medium. The limitations of the work parameters (potential-current) were found to be dependent upon the cell type.
Popa
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C
Department of Materials Science and Technology, Technical University of Cluj-Napoca, Cluj-Napoca, Romania. catalin.popa@stm.utcluj.ro
Su
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B
Vadgama
P
P
Cotter
F
F
D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D055590:Electromagnetic Phenomena; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D006112:Gravitation; D006801:Humans; D008214:Lymphocytes; D008839:Microelectrodes; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D008863:Microspheres