Characterization of a Trypanosoma cruzi acetyltransferase: cellular location, activity and structure.
Trypanosomatids are widespread parasites that cause three major tropical diseases. In trypanosomatids, as in most other organisms, acetylation is a common protein modification that is important in multiple, diverse processes. This paper describes a new member of the Trypanosoma cruzi acetyltransferase family. The gene is single copy and orthologs are also present in the other two sequenced trypanosomatids, Trypanosoma brucei and Leishmania major. This protein (TcAT-1) has the essential motifs present in members of the GCN5-related acetyltransferase (GNAT) family, as well as an additional motif also found in some enzymes from plant and animal species. The protein is evolutionarily more closely related to this group of enzymes than to histone acetyltransferases. The native protein has a cytosolic cellular location and is present in all three life-cycle stages of the parasite. The recombinant protein was shown to have autoacetylation enzymatic activity.
Ochaya
Stephen
S
Department of Cell and Molecular Biology, Karolinska Institutet, Berzelius väg 35, Stockholm 17177, Sweden.
Respuela
Patricia
P
Simonsson
Maria
M
Saraswathi
Abhiman
A
Branche
Carole
C
Lee
Jennifer
J
Búa
Jacqueline
J
Nilsson
Daniel
D
Aslund
Lena
L
Bontempi
Esteban J
EJ
Andersson
Björn
B
D000107:Acetylation; D000123:Acetyltransferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D003593:Cytoplasm / Q000378:metabolism; D017125:Genes, Protozoan; D008969:Molecular Sequence Data; D010802:Phylogeny; D026723:Polyadenylation; D017433:Protein Structure, Secondary; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D014349:Trypanosoma cruzi / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism
D012333:RNA, Messenger; D011994:Recombinant Proteins; D000123:Acetyltransferases