title: Microarray analysis of apple gene expression engaged in early fruit development.
abstract: To evaluate gene expressions mostly engaged in early development of apple fruit, we performed the identification of transcripts differentially expressed in young fruit by using microarrays spotted with 6,253 cDNAs collected from young and mature apple fruits of the cultivar Fuji (Malus domestica Borkh. cv. Fuji). A total of 3,484 cDNAs out of 6,253 were selected after quality control of microarray spots and analyzed for differential gene expression patterns between young fruit and other tissues (mature fruit, leaf and flower). Among them, 192 cDNAs displayed a signal value higher than twofold in young fruit compared with other tissues. Blast analysis of the 192 cDNA clones identified 88 non-redundant groups encoding proteins with known function and 50 non-redundant groups with unknown function. The putative protein products were classified into the following categories: photosynthesis (16.7%), protein synthesis (12.3%), cell proliferation and differentiation (10.9%), cell enlargement (5.8%), metabolism (8.0%), stress response (7.2%), others (2.9%), and unknown functions (32.2%). Furthermore, confirming the microarray data by reverse transcription-polymerase chain reaction revealed that the wide range of transcripts differentially expressed in young fruit was expressed in other organs but not in the mature fruit. The data presented suggested that apple fruit development depends on the tight regulation of the expression of a number of genes, which are also expressed in other organs.
authors.affiliation: Biotech Application Team, Dongbu Advanced Research Institute, Daejeon 305-708, South Korea.
authors.forename: Young-Pyo
authors.initials: YP
authors.lastname: Lee
authors.forename: Gyung-Hee
authors.initials: GH
authors.lastname: Yu
authors.forename: Young Sam
authors.initials: YS
authors.lastname: Seo
authors.forename: Sang Eun
authors.initials: SE
authors.lastname: Han
authors.forename: Yeon-Ok
authors.initials: YO
authors.lastname: Choi
authors.forename: Daeil
authors.initials: D
authors.lastname: Kim
authors.forename: Il-Gin
authors.initials: IG
authors.lastname: Mok
authors.forename: Woo Taek
authors.initials: WT
authors.lastname: Kim
authors.forename: Soon-Kee
authors.initials: SK
authors.lastname: Sung
mesh_terms: D005638:Fruit / Q000235:genetics* / Q000254:growth & development*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D027845:Malus / Q000235:genetics* / Q000254:growth & development*; D020411:Oligonucleotide Array Sequence Analysis; D013997:Time Factors