Usefulness of argyrophilic nucleolar organizer regions in detection of lung cells alterations after benzo[a]pyrene instillation.
OBJECTIVE
To verify the relationship between AgNOR expression and lung tissues changes of Wistar rats after pulmonary instillation of benzo[a]pyrene (B[a]P).


METHODS
Male Rattus norvegicus albinus,Wistar lineage were given a single intrapulmonary instillation of B[a]P at doses of 10 and 20 mg/kg in a volume of approximately 0.3 ml. After 7 and 21 days the rats were killed and the lung slices submitted to a histological technique of AgNOR. AgNOR dots were quantified and the result analyzed by statistical tests; p < or = 0.05 was considered significant.


RESULTS
The mean values of AgNOR dots for the experimental groups 10/7 (1.51+/-0.86) and 10/21 (1.84+/-0.13) were statistically different (p = 0.009). Among the groups 20/7 (1.63+/-0.11) and 20/21 (2.48+/-0.28) was observed statistically significant difference (p = 0.003).


CONCLUSIONS
The AgNOR technique can be useful in identification of cells changes induced by B[a]P.
Silva
Baldomero Antonio Kato da
BA
Postgraduate Program in Health Sciences in the Central West Region UnB and UFMS, Brazil.
Silva
Iandara Schettert
IS
Pereira
Daniel Martins
DM
Aydos
Ricardo Dutra
RD
Carvalho
Paulo de Tarso Camillo de
Pde T
D000818:Animals; D034961:Antigens, Nuclear / Q000378:metabolism; D001564:Benzo(a)pyrene; D014408:Biomarkers, Tumor; D002273:Carcinogens; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D049109:Cell Proliferation / Q000187:drug effects; D008175:Lung Neoplasms / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D008297:Male; D009687:Nuclear Proteins / Q000378:metabolism; D009697:Nucleolus Organizer Region / Q000473:pathology*; D011379:Prognosis; D051381:Rats; D017208:Rats, Wistar; D016622:Silver Staining
D034961:Antigens, Nuclear; D014408:Biomarkers, Tumor; D002273:Carcinogens; D009687:Nuclear Proteins; C051963:nucleolar organizer region associated proteins; D001564:Benzo(a)pyrene