Sulfated glycopolymer thin films - preparation, characterization, and biological activity.
The impact of heparinoid characteristics on model surfaces obtained from immobilization of sole sulfate groups as well as sulfated glycosides, sulfated cellulose, and definite heparin has been investigated. The obtained layers were physico-chemically characterized regarding film thickness, chemical composition, wettability, and surface morphology. Antithrombin adsorption, studied by fluorescence labeling, revealed a strong dependence on the presence of glycosidic structures and on the molecular weight of the grafted saccharide. On contact with whole blood, the coatings resulted in a diminished plasmatic and cellular coagulation in vitro, which did not reflect well the antithrombin binding. Therefore, more complex activating pathways are discussed.
Grombe
Ringo
R
Leibniz Institute of Polymer Research, Max Bergmann Center of Biomaterials, Hohe Strasse 6, 01069 Dresden, Germany.
Gouzy
Marie F
MF
Maitz
Manfred F
MF
Freundenberg
Uwe
U
Zschoche
Stefan
S
Simon
Frank
F
Pompe
Tilo
T
Sperling
Claudia
C
Werner
Carsten
C
D000991:Antithrombins / Q000737:chemistry; D032701:Biomimetics / Q000379:methods*; D001777:Blood Coagulation; D002482:Cellulose / Q000737:chemistry; D006027:Glycosides / Q000737:chemistry; D006493:Heparin / Q000737:chemistry*; D008299:Maleic Anhydrides / Q000737:chemistry*; D018613:Microscopy, Confocal; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry*; D013329:Structure-Activity Relationship; D013463:Sulfuric Acid Esters / Q000737:chemistry*
D000991:Antithrombins; D006027:Glycosides; D008299:Maleic Anhydrides; D011108:Polymers; D013463:Sulfuric Acid Esters; D002482:Cellulose; D006493:Heparin