Substrate supply for effective biocatalysis.
Using biocatalysis for some chemical synthesis steps has unique advantages such as achieving higher product selectivity under ambient process conditions. However, a common limitation with such systems is the inhibition or toxicity posed by the starting substrate as well as limited aqueous solubility in many cases. In this review, we discuss the supply of substrate to bioconversions. The delivery of substrate via an auxiliary, which may be water-miscible, or a second phase such as a water-immiscible organic solvent, adsorbing resin, or a gas, is examined through recent examples in the field. Finally, guidelines for experimental planning and process considerations are suggested to facilitate the choice of substrate delivery method and accelerate process development.
Kim
Pei-Yi
PY
Department of Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, United Kingdom.
Pollard
David J
DJ
Woodley
John M
JM
D002384:Catalysis; D004798:Enzymes / Q000737:chemistry* / Q000378:metabolism*; D013379:Substrate Specificity; D013678:Technology, Pharmaceutical / Q000639:trends*
D004798:Enzymes