From exogenous to endogenous: the inevitable imprint of mass spectrometry in metabolomics.
Mass spectrometry (MS) is an established technology in drug metabolite analysis and is now expanding into endogenous metabolite research. Its utility derives from its wide dynamic range, reproducible quantitative analysis, and the ability to analyze biofluids with extreme molecular complexity. The aims of developing mass spectrometry for metabolomics range from understanding basic biochemistry to biomarker discovery and the structural characterization of physiologically important metabolites. In this review, we will discuss the techniques involved in this exciting area and the current and future applications of this field.
Want
Elizabeth J
EJ
Department of Molecular Biology, The Scripps Center for Mass Spectrometry, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Nordström
Anders
A
Morita
Hirotoshi
H
Siuzdak
Gary
G
D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D013058:Mass Spectrometry / Q000379:methods; D008660:Metabolism; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D020543:Proteome
D011506:Proteins; D020543:Proteome