Study of superoxide dismutase's expression in the colon produced by azoxymethane and inositol hexaphosfate's paper, in mice.
OBJECTIVE
To investigate the expression of superoxide dismutase (SOD), with use of antioxidant inositol hexaphosfate, in the presence of the carcinogen azoxymethane, in FCA of colon rats.


METHODS
Wistar rats (n=48) were distributed in four groups of 12 mice. Divided in control (n=12); with azoxymethane administration AOM (n=12); administration of IP6 (n=12) and with administration of IP6/AOM (n=12). The subcutaneous administration of azoxymethane happened in the week 3 and 4 of the experiment, in dose 20 mg/Kg, weekly; and administration of IP6 to 1% in water of drinking for 6 weeks in the group 3 and 4. The identification of the expression SOD-1 was accomplished through the quantification imunohistochemistry by the image processing attended by computer in crypts and focus of aberrant crypts in right colon.


RESULTS
The group control presented expression of SOD1, on average 16.0%; group AOM, 26.7%; group IP6, 16.9%; group IP6/AOM, 20.9%. Variance analysis among the groups, was calculated 0.0078.


CONCLUSIONS
The azoxymethane increase expression SOD1, while inositol hexaphosphate decreases in a significant way the expression of SOD1 promoted by the administration of the carcinogen azoxymethane.
Amaral
Eva Glória Abrão Siufi do
EG
Department of Surgery, Federal University of Mato Grosso do Sul, Brazil.
Fagundes
Djalma José
DJ
Marks
Guido
G
Inouye
Celso Massachi
CM
D000818:Animals; D001397:Azoxymethane / Q000633:toxicity*; D002273:Carcinogens / Q000633:toxicity*; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000201:enzymology* / Q000517:prevention & control; D008297:Male; D010833:Phytic Acid / Q000494:pharmacology*; D011230:Precancerous Conditions / Q000139:chemically induced / Q000517:prevention & control; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism*
D002273:Carcinogens; D010833:Phytic Acid; D013482:Superoxide Dismutase; D001397:Azoxymethane