title: Endocrine function and lymphocyte infiltration of newborn rat ovaries after ultrarapid freezing and allotransplantation.
abstract: OBJECTIVE
  To evaluate the endocrine function and lymphocyte infiltration of ultrarapidly frozen newborn rat ovaries in adult recipients.
  
  
  METHODS
  Animal study.
  
  
  METHODS
  Reproductive Medicine Laboratory of Ningxia Medical College.
  
  
  METHODS
  Newborn rats within 24 hours after birth and Sprague-Dawley female adult rats.
  
  
  METHODS
  Newborn or adult rat ovary tissues were cryopreserved with ultrarapid freezing method, using 1.5 M propylene glycol and 0.1 M sucrose as a cryoprotectant agent. After thawing, they were allotransplanted under the kidney capsule of ovariectomized adult female rats to assess the function and the microstructure.
  
  
  METHODS
  Vaginal smear, serum E2 level in recipients, grafts histologic observation, 3beta-hydroxy steroid dehydrogenase histochemistry staining, and CD4(+)/CD8(+) T cell immunohistochemistry staining.
  
  
  RESULTS
  Frozen-thawed newborn rat ovaries survived and established a vascular network with the kidney of recipients. More growing follicles were found in these survival grafts. No significant differences were found in both resumption rates, the day of initiating estrous cycles, and E2 level between the frozen adult and frozen newborn rat ovaries transplant group. Among all the groups, the number of lymphocyte in the frozen newborn rat ovary transplant group is the lowest.
  
  
  CONCLUSIONS
  This is the first report for ultrarapid cryopreservation that can preserve the development potential of immature ovaries in ovariectomized adult recipients and further reduce their immunogenicity successfully.
authors.affiliation: Department of Histology and Embryology, Ningxia Medical College, Yinchuan, Ningxia, People's Republic of China. 4083304@163.com
authors.forename: Yanrong
authors.initials: Y
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Shen
authors.forename: Chengjun
authors.initials: C
authors.lastname: Zhao
authors.forename: Xiqing
authors.initials: X
authors.lastname: Zhao
authors.forename: Yueping
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes; D004958:Estradiol / Q000097:blood; D005260:Female; D005615:Freezing; D006728:Hormones / Q000502:physiology*; D008214:Lymphocytes / Q000502:physiology*; D010052:Ovariectomy; D010053:Ovary / Q000254:growth & development / Q000502:physiology* / Q000637:transplantation*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014184:Transplantation, Homologous; D014626:Vaginal Smears
chemical_list: D006728:Hormones; D004958:Estradiol