Iron-sulfur cluster stability. Kinetics and mechanism of ligand-promoted cluster degradation.
Reactivity studies of iron-sulfur cluster proteins with chelating ligands model the reactivity of cluster scaffold proteins such as ISU, and suggest a rate law [k(obs) = k2[ligand]n/{[ligand]n + K(D)}] consistent with formation of a pre-reaction complex between the Fe-S protein and one chelate ligand.
Wu
Shu-pao
SP
Evans Laboratory of Chemistry, Ohio State University, 100 West, 18th Ave, Columbus, OH 43210, USA.
Cowan
J A
JA
D014013:1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt / Q000737:chemistry; D015082:2,2'-Dipyridyl / Q000737:chemistry; D019943:Amino Acid Substitution; D002614:Chelating Agents / Q000737:chemistry*; D005288:Ferredoxins / Q000737:chemistry; D006801:Humans; D007501:Iron / Q000737:chemistry; D007506:Iron-Sulfur Proteins / Q000737:chemistry* / Q000235:genetics; D007700:Kinetics; D008024:Ligands; D012568:Schizosaccharomyces / Q000737:chemistry; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet
D002614:Chelating Agents; D005288:Ferredoxins; D007506:Iron-Sulfur Proteins; D008024:Ligands; D014013:1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt; D015082:2,2'-Dipyridyl; D007501:Iron