Depuration dynamics of oysters (Crassostrea gigas) artificially contaminated by Salmonella enterica serovar Typhimurium.
The State of Santa Catarina produces the greatest quantity of edible mollusks in Brazil. To guarantee sanitary qualify, mollusk cultures should be monitored for contamination by pathogenic microorganisms. A self-purification or "depuration" system that eliminates Salmonella enterica serovar Typhimurium contamination from oysters has been developed and evaluated. The depuration process occurred within a closed system, in which 1000 L of water was recirculated for 24 h. The water was sterilized with ultraviolet (UV) light, chlorine, or both together. Oysters (Crassostrea gigas) artificially contaminated with S. typhimurium were harvested every 6 h. Samples of oyster tissue were excised and both the presence and numbers of bacteria were determined. Combined UV light and chlorine treatments resulted in total elimination of bacteria within 12 h. Polymerase chain reaction detected bacteria in water exposed to the three treatments. This pioneering study is the first of its kind in Brazil and represents a major contribution to commercial mollusk culture in this country.
de Abreu Corrêa
Adriana
A
Laboratório de Virologia Aplicada, Departamento de Microbiologia e Parasitologia, CCB, Campus Universitario Trindade, 88040-900 Florianópolis-SC, Brazil.
Albarnaz
Jonas Dutra
JD
Moresco
Vanessa
V
Poli
Carlos Rogério
CR
Teixeira
Adriano Luiz
AL
Oliveira Simões
Cláudia Maria
CM
Monte Barardi
Célia Regina
CR
D000704:Analysis of Variance; D000818:Animals; D017756:Aquaculture / Q000379:methods*; D001938:Brazil; D002713:Chlorine / Q000633:toxicity; D017931:DNA Primers; D004586:Electrophoresis; D005506:Food Contamination / Q000517:prevention & control*; D010125:Ostreidae / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics / Q000528:radiation effects*; D012758:Shellfish / Q000382:microbiology*; D014466:Ultraviolet Rays
D017931:DNA Primers; D002713:Chlorine