A challenging insight on the structural unit 1 of molluscan Rapana venosa hemocyanin.
Hemocyanins of mollusks are high molecular mass glycoproteins with a complex quaternary structure which still remains to be defined in detail for most of its species as far as number, spatial distribution and interactions of their structural units is concerned. In the present study, we isolated the functional units of the structural subunit RvH1 of Rapana venosa hemocyanin, combining enzymatic and non-enzymatic methods. Our results suggest that Hc's carbohydrate moieties play a basic role in the organization of the structural units, resulting from post-translational polymerization of the 50 kDa functional units and involving sugar moieties that link between them.
Dolashka-Angelova
Pavlina
P
Institute of Organic Chemistry, Bulgarian Academy of Sciences, G. Bonchev 9, Sofia 1113, Bulgaria. pda54@yahoo.com
Stevanovic
Stefan
S
Dolashki
Alexandar
A
Devreese
Bart
B
Tzvetkova
Bonka
B
Voelter
Wolfgang
W
Van Beeumen
Jozef
J
Salvato
Benedetto
B
D000595:Amino Acid Sequence; D000818:Animals; D006433:Hemocyanins / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D008974:Mollusca / Q000201:enzymology*; D013329:Structure-Activity Relationship; D014508:Urea / Q000737:chemistry*
D014508:Urea; D006433:Hemocyanins