title: [Study on E-cadherin, Cyclin D1 and Cyclin E expression in anti-malignant transformation by chlorophyllin].
abstract: OBJECTIVE
  To investigate the effects of chlorophyllin on the regulations of proteins related to cell cycle in vitro in human bronchial epithelial cell line 16HBE transformed by trans-benzo(a) pyrene-trans-7, 8-dihydrodiol-9, 10-epoxide (trans-BPDE).
  
  
  METHODS
  RT-PCR and fluoroimmunocytochemistry methods were used to detect the expression of E-cadherin, in mRNA and protein levels, among untreated control cells, malignant transformed cells induced by trans-BPDE and anti-transformed cells treated with chlorophyllin. The expression of cyclins such as Cyclin D1 and Cyclin E was also detected by fluoroimmunocytochemistry method.
  
  
  RESULTS
  The loss of E-cadherin expression was found in mRNA and protein levels after being transformed by trans-BPDE, while its expression existed normally after being anti-transformed by chlorophyllin. Cyclin D1 and Cyclin E had no expression in control 16HBE, but expressions of the proteins were enhanced obviously in malignant transformed cell line while those were inhibited significantly in the anti-transformed cells treated with 100pmol/L chlorophyllin.
  
  
  CONCLUSIONS
  The ability of chlorophyllin to anti-malignant transformants 16HBE cells exposed to trans-BPDE is correlated with arrest the loss of E-cadherin expression. Chlorophyllin has a significant effect on expressions of Cyclin Dl and Cyclin E during the course of anti-malignant transformation.
authors.affiliation: Guangzhou Medical College, Guangzhou 510182, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Fu
authors.forename: Yi-guo
authors.initials: YG
authors.lastname: Jiang
authors.forename: Xiao-nong
authors.initials: XN
authors.lastname: Bin
authors.forename: Xue-min
authors.initials: XM
authors.lastname: Chen
mesh_terms: D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; D016587:Antimutagenic Agents / Q000494:pharmacology*; D015820:Cadherins / Q000096:biosynthesis* / Q000235:genetics; D002273:Carcinogens; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002735:Chlorophyllides / Q000494:pharmacology*; D019938:Cyclin D1 / Q000096:biosynthesis* / Q000235:genetics; D019927:Cyclin E / Q000096:biosynthesis* / Q000235:genetics; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D016587:Antimutagenic Agents; D015820:Cadherins; D002273:Carcinogens; D002735:Chlorophyllides; D019927:Cyclin E; D012333:RNA, Messenger; D019938:Cyclin D1; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; C007020:chlorophyllin