Combined use of 2,4,6-trihydroxyacetophenone as matrix and enzymatic deglycosylation in organic-aqueous solvent systems for the simultaneous characterization of complex glycoproteins and N-glycans by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
Fenaille
François
F
Le Mignon
Maxime
M
Groseil
Catherine
C
Siret
Laurent
L
Bihoreau
Nicolas
N
D000098:Acetophenones / Q000737:chemistry*; D000911:Antibodies, Monoclonal / Q000737:chemistry; D000991:Antithrombins / Q000737:chemistry; D017242:Complement Factor H / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry*; D006031:Glycosylation; D011134:Polysaccharides / Q000737:chemistry*; D011516:Prothrombin / Q000737:chemistry; D012260:Ribonucleases / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D000098:Acetophenones; D000911:Antibodies, Monoclonal; D000991:Antithrombins; C493495:CFH protein, human; D006023:Glycoproteins; D011134:Polysaccharides; D017242:Complement Factor H; C107067:2,4,6-trihydroxyacetophenone; D011516:Prothrombin; D012260:Ribonucleases; C029600:ribonuclease B