title: High-throughput genotyping of filamentous fungus Aspergillus oryzae based on colony direct polymerase chain reaction.
abstract: High-throughput genotyping of Aspergillus oryzae was achieved using an FTA card for the extraction of a genomic DNA template for polymerase chain reaction from a fungal colony growing on an agar plate. This method was then applied to detect other fungal species from agar slants and food materials. This method offers a convenient tool for the genotyping of filamentous fungi without using an organic solvent or specialized equipment.
authors.affiliation: National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan. satosuz@affrc.go.jp
authors.forename: Satoshi
authors.initials: S
authors.lastname: Suzuki
authors.forename: Hiroko
authors.initials: H
authors.lastname: Taketani
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Kusumoto
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kashiwagi
mesh_terms: D001236:Aspergillus oryzae / Q000235:genetics* / Q000302:isolation & purification*; D002874:Chromosome Mapping / Q000379:methods*; D004271:DNA, Fungal / Q000235:genetics*; D016681:Genome, Fungal / Q000235:genetics*; D005838:Genotype; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004271:DNA, Fungal