[Study on E-cadherin, Cyclin D1 and Cyclin E expression in anti-malignant transformation by chlorophyllin].
OBJECTIVE
To investigate the effects of chlorophyllin on the regulations of proteins related to cell cycle in vitro in human bronchial epithelial cell line 16HBE transformed by trans-benzo(a) pyrene-trans-7, 8-dihydrodiol-9, 10-epoxide (trans-BPDE).


METHODS
RT-PCR and fluoroimmunocytochemistry methods were used to detect the expression of E-cadherin, in mRNA and protein levels, among untreated control cells, malignant transformed cells induced by trans-BPDE and anti-transformed cells treated with chlorophyllin. The expression of cyclins such as Cyclin D1 and Cyclin E was also detected by fluoroimmunocytochemistry method.


RESULTS
The loss of E-cadherin expression was found in mRNA and protein levels after being transformed by trans-BPDE, while its expression existed normally after being anti-transformed by chlorophyllin. Cyclin D1 and Cyclin E had no expression in control 16HBE, but expressions of the proteins were enhanced obviously in malignant transformed cell line while those were inhibited significantly in the anti-transformed cells treated with 100pmol/L chlorophyllin.


CONCLUSIONS
The ability of chlorophyllin to anti-malignant transformants 16HBE cells exposed to trans-BPDE is correlated with arrest the loss of E-cadherin expression. Chlorophyllin has a significant effect on expressions of Cyclin Dl and Cyclin E during the course of anti-malignant transformation.
Fu
Juan
J
Guangzhou Medical College, Guangzhou 510182, China.
Jiang
Yi-guo
YG
Bin
Xiao-nong
XN
Chen
Xue-min
XM
D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; D016587:Antimutagenic Agents / Q000494:pharmacology*; D015820:Cadherins / Q000096:biosynthesis* / Q000235:genetics; D002273:Carcinogens; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002735:Chlorophyllides / Q000494:pharmacology*; D019938:Cyclin D1 / Q000096:biosynthesis* / Q000235:genetics; D019927:Cyclin E / Q000096:biosynthesis* / Q000235:genetics; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D016587:Antimutagenic Agents; D015820:Cadherins; D002273:Carcinogens; D002735:Chlorophyllides; D019927:Cyclin E; D012333:RNA, Messenger; D019938:Cyclin D1; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; C007020:chlorophyllin