title: Natural chlorophyll inhibits aflatoxin B1-induced multi-organ carcinogenesis in the rat.
abstract: Chemoprevention by chlorophyll (Chl) was investigated in a rat multi-organ carcinogenesis model. Twenty-one male F344 rats in three gavage groups (N = 7 rats each) received five daily doses of 250 microg/kg [(3)H]-aflatoxin B(1) ([(3)H]-AFB(1)) alone, or with 250 mg/kg chlorophyllin (CHL), or an equimolar amount (300 mg/kg) of Chl. CHL and Chl reduced hepatic DNA adduction by 42% (P = 0.031) and 55% (P = 0.008), respectively, AFB(1)-albumin adducts by 65% (P < 0.001) and 71% (P < 0.001), respectively, and the major AFB-N(7)-guanine urinary adduct by 90% (P = 0.0047) and 92% (P = 0.0029), respectively. To explore mechanisms, fluorescence quenching experiments established formation of a non-covalent complex in vitro between AFB(1) and Chl (K(d) = 1.22 +/- 0.05 microM, stoichiometry = 1Chl:1AFB(1)) as well as CHL (K(d) = 3.05 +/- 0.04 microM; stoichiometry = 1CHL:1AFB(1)). The feces of CHL and Chl co-gavaged rats contained 137% (P = 0.0003) and 412% (P = 0.0048) more AFB(1) equivalents, respectively, than control feces, indicating CHL and Chl inhibited AFB(1) uptake. However, CHL or Chl treatment in vivo did not induce hepatic quinone reductase (NAD(P)H:quinone oxidoreductase) or glutathione S-transferase (GST) above control levels. These results are consistent with a mechanism involving complex-mediated reduction of carcinogen uptake, and do not support a role for phase II enzyme induction in vivo under these conditions. In a second study, 30 rats in three experimental groups were dosed as in study 1, but for 10 days. At 18 weeks, CHL and Chl had reduced the volume percent of liver occupied by GST placental form-positive foci by 74% (P < 0.001) and 77% (P < 0.001), respectively compared with control livers. CHL and Chl reduced the mean number of aberrant crypt foci per colon by 63% (P = 0.0026) and 75% (P = 0.0004), respectively. These results show Chl and CHL provide potent chemoprotection against early biochemical and late pathophysiological biomarkers of AFB(1) carcinogenesis in the rat liver and colon.
authors.affiliation: Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA.
authors.forename: Michael T
authors.initials: MT
authors.lastname: Simonich
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Egner
authors.forename: Bill D
authors.initials: BD
authors.lastname: Roebuck
authors.forename: Gayle A
authors.initials: GA
authors.lastname: Orner
authors.forename: Carole
authors.initials: C
authors.lastname: Jubert
authors.forename: Cliff
authors.initials: C
authors.lastname: Pereira
authors.forename: John D
authors.initials: JD
authors.lastname: Groopman
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Kensler
authors.forename: Roderick H
authors.initials: RH
authors.lastname: Dashwood
authors.forename: David E
authors.initials: DE
authors.lastname: Williams
authors.forename: George S
authors.initials: GS
authors.lastname: Bailey
mesh_terms: D016604:Aflatoxin B1 / Q000037:antagonists & inhibitors* / Q000633:toxicity; D000818:Animals; D016588:Anticarcinogenic Agents / Q000008:administration & dosage* / Q000627:therapeutic use; D002273:Carcinogens / Q000037:antagonists & inhibitors* / Q000633:toxicity; D002734:Chlorophyll / Q000008:administration & dosage* / Q000502:physiology* / Q000627:therapeutic use; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D008113:Liver Neoplasms / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D008297:Male; D011897:Random Allocation; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D016588:Anticarcinogenic Agents; D002273:Carcinogens; D002734:Chlorophyll; D016604:Aflatoxin B1