Isolation of the rstA gene as a multicopy suppressor of YjeE, an essential ATPase of unknown function in Escherichia coli.
YjeE is an essential ATPase in Escherichia coli whose cellular function remains uncharacterized. Using a genomic library, we have identified rstA as a multicopy suppressor of a conditional yjeE deletion strain. High-copy rstA is the first recorded suppressor for a lesion in yjeE, and this newly charted genetic interaction has the potential to be informative about the function, with further study of the interacting partners.
Campbell
Tracey L
TL
Antimicrobial Research Centre, Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main St. West, Hamilton, Ontario, Canada.
Ederer
Claudia S
CS
Allali-Hassani
Abdellah
A
Brown
Eric D
ED
D000251:Adenosine Triphosphatases / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D029968:Escherichia coli Proteins / Q000378:metabolism; D005798:Genes, Bacterial; D016153:Genes, Suppressor; D015698:Genomic Library
D029968:Escherichia coli Proteins; C500230:YjeE protein, E coli; D000251:Adenosine Triphosphatases