Spectrophotometric determination of uric acid based on fading of o-hydroxyhydroquinonephthalein-palladium(II)-hexadecyltrimethyl-ammonium complex.
A simple and highly sensitive spectrophotometric method for the determination of uric acid (UA) was established based on fading of the o-hydroxyhydroquinonephthalein-palladium(II)-hexadecyltrimethylammonium complex. In the determination of UA, Beer's law is obeyed in the range of 0.01-0.20 microg ml(-1), with an effective molar absorptivity at 635 nm, the relative standard deviation being 6.5 x 10(5) dm(3) mol(-1) cm(-1) and 1.5% (n = 5). This method is about 20-times more sensitive than the conventional methods. The method was successfully applied to the assay of UA in human urine.
Yamaguchi
Takako
T
Osaka University of Pharmaceutical Sciences, Takatsuki, Osaka, Japan.
Hasegawa
Kohei
K
Kamino
Shinichiro
S
Miyachi
Kanako
K
Tominaga
Hiroshi
H
Fujita
Yoshikazu
Y
D016477:Artifacts; D002138:Calibration; D002413:Cations, Divalent; D004396:Coloring Agents / Q000737:chemistry; D006801:Humans; D006873:Hydroquinones / Q000737:chemistry*; D008670:Metals / Q000737:chemistry; D009941:Organomercury Compounds / Q000737:chemistry*; D010165:Palladium / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013053:Spectrophotometry; D013997:Time Factors; D014527:Uric Acid / Q000652:urine*
D002413:Cations, Divalent; D004396:Coloring Agents; D006873:Hydroquinones; D008670:Metals; D009941:Organomercury Compounds; D000644:Quaternary Ammonium Compounds; C039209:2-hydroxyhydroquinonephthalein; D014527:Uric Acid; D010165:Palladium