Formation of high-resistance supported lipid bilayer on the surface of a silicon substrate with microelectrodes.
We have developed two basic technologies for fabrication of supported planar lipid bilayer membrane ion channel biosensors: a defect-free lipid bilayer formation on the substrate surface with electrode pores and a patterning technique for the hydrophobic self-assembled-monolayer to form the guard ring that reduces the lipid bilayer edge-leak current. The importance of the supported-membrane structure to achieve low noise and high-speed performance is suggested on the basis of the observed relation between the single-ion-channel current noise and the pore size.
Urisu
Tsuneo
T
Department of Vacuum UV Photoscience, Institute for Molecular Science, The Graduate University for Advanced Studies, Myodaiji, Okazaki, Japan. urisu@ims.ac.jp
Rahman
Md Mashiur
MM
Uno
Hidetaka
H
Tero
Ryugo
R
Nonogaki
Yoichi
Y
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D003460:Crystallization / Q000379:methods; D017097:Electric Impedance; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D006096:Gramicidin / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D008839:Microelectrodes; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D010316:Particle Size; D013499:Surface Properties
D008051:Lipid Bilayers; D006096:Gramicidin