Potency of authentic nitric oxide in inducing aortic relaxation.
The reported EC(50) of vasorelaxation for authentic nitric oxide (NO) is from the nM to muM range. The cause of this large difference is undetermined. In this study, NO electrodes were used to monitor the actual NO concentration in the organ chamber during the recording of the relaxation of rat aortic rings. It was demonstrated that both the O(2) concentration in the solution and the rate of stirring the solution markedly affected the actual NO concentration, while the vasorelaxation response to NO was also changed with the composition of the buffer solution. It was observed that the apparent EC(50) of aortic relaxation for authentic NO is 340+/-40nM and 81+/-4nM in the conventional organ chamber containing Krebs-Ringer buffer bubbled with a high content (95% O(2)+5% CO(2)) and a low content (20% O(2)+5% CO(2)+75% N(2)) of O(2) gas mixture, respectively. The apparent EC(50) was further reduced to 45+/-2nM after the Krebs-Ringer buffer, a bicarbonate buffer, was replaced by a phosphate buffer solution bubbled with a low content of O(2) gas mixture (20% O(2)+80% N(2)) or with air. By using an organ chamber, which makes low concentrations of NO more stable in the solution, it was determined that the apparent EC(50) was 9.7+/-0.4nM and the threshold of NO concentration in dilating the aortas was approximately 0.3nM. This modified organ chamber will be useful for quantitatively measuring EC(50) of vasorelaxation for authentic NO under physiological and pathological conditions.
Yan
Qingtao
Q
Davis Heart and Lung Research Institute, The Division of Cardiovascular Medicine, Department of Internal Medicine, and Biomedical Engineering Department, The Ohio State University, 473 W. 12th Avenue, Columbus, OH 43210, United States.
Liu
Qihui
Q
Zweier
Jay L
JL
Liu
Xiaoping
X
D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects / Q000378:metabolism*; D002021:Buffers; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D045462:Endothelium-Dependent Relaxing Factors / Q000378:metabolism* / Q000494:pharmacology; D004867:Equipment Design; D066298:In Vitro Techniques; D017736:Ion-Selective Electrodes; D007552:Isotonic Solutions / Q000737:chemistry; D009569:Nitric Oxide / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism; D010313:Partial Pressure; D051381:Rats; D011921:Rats, Inbred WKY; D013997:Time Factors; D014664:Vasodilation / Q000187:drug effects
D002021:Buffers; D045462:Endothelium-Dependent Relaxing Factors; D007552:Isotonic Solutions; C051865:Krebs-Ringer solution; D009569:Nitric Oxide; D010100:Oxygen