Sulfite reductase-mediated synthesis of gold nanoparticles capped with phytochelatin.
An enzymatic synthesis route to peptide-capped gold nanoparticles has been developed. Gold nanoparticles were synthesized using alpha-NADPH-dependent sulfite reductase and phytochelatin in vitro. The gold ions were reduced in the presence of the enzyme sulfite reductase, leading to the formation of a stable gold hydrosol of dimensions 7-20 nm and were stabilized by the capping peptide. The nanoparticles were characterized by X-ray diffraction, transmission electron microscopy, X-ray photoelectron spectroscopy and UV-visible optical absorption. These studies will help in designing a rational enzymatic strategy for the synthesis of nanomaterials of different chemical compositions, shapes and sizes as well as their separation.
Kumar
Suresh Anil
SA
Biochemical Sciences Division, National Chemical Laboratory, Pune, India.
Abyaneh
Majid Kazemian
MK
Gosavi
Suresh W
SW
Kulkarni
Sulabha K
SK
Ahmad
Absar
A
Khan
M Islam
MI
D000327:Adsorption; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005978:Glutathione / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D054811:Phytochelatins; D050864:Sulfite Reductase (NADPH) / Q000737:chemistry*; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; D006046:Gold; D054811:Phytochelatins; D050864:Sulfite Reductase (NADPH); D005978:Glutathione