title: Sulfite reductase-mediated synthesis of gold nanoparticles capped with phytochelatin.
abstract: An enzymatic synthesis route to peptide-capped gold nanoparticles has been developed. Gold nanoparticles were synthesized using alpha-NADPH-dependent sulfite reductase and phytochelatin in vitro. The gold ions were reduced in the presence of the enzyme sulfite reductase, leading to the formation of a stable gold hydrosol of dimensions 7-20 nm and were stabilized by the capping peptide. The nanoparticles were characterized by X-ray diffraction, transmission electron microscopy, X-ray photoelectron spectroscopy and UV-visible optical absorption. These studies will help in designing a rational enzymatic strategy for the synthesis of nanomaterials of different chemical compositions, shapes and sizes as well as their separation.
authors.affiliation: Biochemical Sciences Division, National Chemical Laboratory, Pune, India.
authors.forename: Suresh Anil
authors.initials: SA
authors.lastname: Kumar
authors.forename: Majid Kazemian
authors.initials: MK
authors.lastname: Abyaneh
authors.forename: Suresh W
authors.initials: SW
authors.lastname: Gosavi
authors.forename: Sulabha K
authors.initials: SK
authors.lastname: Kulkarni
authors.forename: Absar
authors.initials: A
authors.lastname: Ahmad
authors.forename: M Islam
authors.initials: MI
authors.lastname: Khan
mesh_terms: D000327:Adsorption; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005978:Glutathione / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D054811:Phytochelatins; D050864:Sulfite Reductase (NADPH) / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D006046:Gold; D054811:Phytochelatins; D050864:Sulfite Reductase (NADPH); D005978:Glutathione