title: Lack of evidence of sustained hematopoietic reconstitution after transplantation of unmanipulated adult liver stem cells in monkeys.
abstract: The aim of this study was to search for hematopoietic potential in the liver of non-human primates. Lethally irradiated (2 x 5 Gy gamma) macaque monkeys were given autologous hepatic mononuclear cells (HMNC) isolated from a liver lobe by perfusion and digestion with 0.1% collagenase. Two monkeys were given intramedullary injections of HMNC (18.6 x 10(6)/kg, 20.4 x 10(6)/kg) and two others were co-transplanted with HMNC (14.35 x 10(6)/kg, 96.5 x 10(6)/kg) and bone marrow mesenchymal stem cells (0.42 x 10(6)/kg, 1.16 x 10(6)/kg). All monkeys exhibited a transient neutrophil recovery from day 22 for 10 days, but failed to produce platelets and remained transfusion-dependent. In conclusion, adult liver stem cells from a monkey model show a low level of in vivo hematopoietic potential, suggesting ex vivo manipulation will be required before clinical use of such cells.
authors.affiliation: Experimental Radiohematology Unit, Centre de Recherches du Service de Santé des Armées, 24 Avenue des Maquis du Grésivaudan, 38702, La Tronche, France. micheldrouet@crssa.net
authors.forename: Michel
authors.initials: M
authors.lastname: Drouet
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Mayol
authors.forename: Françoise
authors.initials: F
authors.lastname: Norol
authors.forename: André
authors.initials: A
authors.lastname: Peinnequin
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Zarski
authors.forename: Christian
authors.initials: C
authors.lastname: Létoublon
authors.forename: Francis
authors.initials: F
authors.lastname: Hérodin
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000378:metabolism; D016026:Bone Marrow Transplantation / Q000379:methods*; D006413:Hematopoietic System; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008099:Liver / Q000166:cytology*; D008110:Liver Extracts / Q000378:metabolism; D008252:Macaca fascicularis; D059630:Mesenchymal Stem Cells / Q000166:cytology; D009504:Neutrophils / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D033581:Stem Cell Transplantation / Q000379:methods*
chemical_list: D008110:Liver Extracts