Oxygenation monitoring of tissue vasculature by resonance Raman spectroscopy.
Resonance Raman spectroscopy offers a mechanism for the noninvasive measurement of in vivo and in situ hemoglobin oxygen saturation (HbO(2)Sat) in living tissue. Clinically informative signals can be provided by resonance enhancement with deep violet excitation. It is notable that fluorescence does not significantly degrade the quality of the signals. During the controlled hemorrhage and resuscitation of rats, signal intensity ratios of oxy- vs. deoxyhemoglobin from sublingual mucosa correlated with co-oximetry values of blood withdrawn from a central venous catheter. The spectroscopic application described here has potential as a noninvasive method for the diagnosis of clinical shock and guidance of its therapy.
Ward
Kevin R
KR
Department of Anesthesiology, Virginia Commonwealth University, Richmond, Virginia 23298, USA.
Barbee
R Wayne
RW
Reynolds
Penny S
PS
Torres Filho
Ivo P
IP
Tiba
M Hakam
MH
Torres
Luciana
L
Pittman
Roland N
RN
Terner
James
J
D000818:Animals; D006801:Humans; D010100:Oxygen / Q000097:blood*; D010108:Oxyhemoglobins / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman / Q000379:methods* / Q000592:standards
D010108:Oxyhemoglobins; D010100:Oxygen