title: Effects of alimentary lipemia and inflammation on platelet CD40-ligand.
abstract: BACKGROUND
  In patients with chronic hypercholesterolemia, the CD40-CD40L dyad is upregulated, contributing to the initiation and progression of atherosclerosis. Our aim was to describe the role of postprandial lipemia and inflammatory stimulation on platelet and monocyte activation and CD40-ligand (CD40L) levels.
  
  
  RESULTS
  Before and 2 h after consumption of a defined fatty meal, whole blood samples of 31 healthy subjects were incubated with endotoxin (LPS). CD40-ligand and CD62P expression on platelets, tissue-factor expression on monocytes and platelet-monocyte aggregates were measured with flow cytometry. Soluble CD40-ligand plasma levels were measured with an ELISA. After the meal, serum triglyceride levels increased from 137.6+/-60.5 mg/dl to 201.5+/-75.0 mg/dl. Expression of CD40L and CD62P on platelets and plasma levels of soluble CD40L were significantly decreased. No significant changes after the meal were observed concerning tissue factor expression on monocytes and platelet-monocyte aggregates. Addition of LPS showed no significant effect concerning CD40L or CD62P expression on platelets, whereas the amount of platelet-monocyte aggregates significantly increased under LPS stimulation after the fatty meal.
  
  
  CONCLUSIONS
  Acute alimenatry lipemia leads to a decreased expression of CD40L on platelets and a reduced plasma level of sCD40L, suggesting an increased turnover in the CD40L system.
  
  
  CONCLUSIONS
  Before and after a fatty meal, blood samples of 31 healthy subjects were incubated with LPS. After the meal, expression of CD40L and CD62P on platelets and plasma levels of soluble CD40L were significantly decreased. Addition of LPS showed no effect concerning CD40L or CD62P expression, whereas the amount of platelet-monocyte aggregates significantly increased under LPS stimulation after the fatty meal.
authors.affiliation: 1st Department of Medicine, University Hospital Mannheim, Medical Faculty Mannheim, University of Heidelberg, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany.
authors.forename: Thorsten
authors.initials: T
authors.lastname: Kälsch
authors.forename: Elif
authors.initials: E
authors.lastname: Elmas
authors.forename: Xuan Duc
authors.initials: XD
authors.lastname: Nguyen
authors.forename: Stefan
authors.initials: S
authors.lastname: Kralev
authors.forename: Hans
authors.initials: H
authors.lastname: Leweling
authors.forename: Harald
authors.initials: H
authors.lastname: Klüter
authors.forename: Carl-Erik
authors.initials: CE
authors.lastname: Dempfle
authors.forename: Martin
authors.initials: M
authors.lastname: Borggrefe
mesh_terms: D000328:Adult; D000368:Aged; D001792:Blood Platelets / Q000378:metabolism*; D019013:CD40 Antigens / Q000096:biosynthesis*; D023201:CD40 Ligand / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood; D006949:Hyperlipidemias / Q000378:metabolism*; D007249:Inflammation; D019041:L-Selectin / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D019518:Postprandial Period; D013925:Thromboplastin / Q000378:metabolism
chemical_list: D019013:CD40 Antigens; D008070:Lipopolysaccharides; D019041:L-Selectin; D023201:CD40 Ligand; D013925:Thromboplastin