The use of functional genomics for the identification of a gene cluster encoding for the biosynthesis of an antifungal tambjamine in the marine bacterium Pseudoalteromonas tunicata.
A large insert library was created in Escherichia coli from the DNA of the surface-associated marine bacterium Pseudoalteromonas tunicata. Screening of the library for antifungal activity resulted in the detection and identification of a large gene cluster encoding for the biosynthesis of an antifungal tambjamine. A biosynthetic pathway has been proposed based on analysis and annotation of the gene cluster.
Burke
Catherine
C
School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney 2052, Australia.
Thomas
Torsten
T
Egan
Suhelen
S
Kjelleberg
Staffan
S
D000935:Antifungal Agents / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D015723:Gene Library; D023281:Genomics; D005810:Multigene Family; D031161:Pseudoalteromonas / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D012623:Seawater / Q000382:microbiology*
D000935:Antifungal Agents; D001426:Bacterial Proteins