Adjuvant effect of green propolis on humoral immune response of bovines immunized with bovine herpesvirus type 5.
Despite recent technological advances in vaccine production, most vaccines depend on the association with adjuvant substances. In this study, propolis, which has been attracting the attention of researchers due to its bioactive properties, was evaluated as an immunological adjuvant. The association of 40mg/dose of an ethanolic extract of green propolis with an inactivated oil vaccine against bovine herpesvirus type 5 (BoHV-5), resulted in a significant increase (P<0.01) in the neutralizing antibody levels, comparing to the bovines that received the same vaccine without propolis. Besides, propolis increased the percentage of animals with high antibody titers (above 32). Phenolic compounds such as artepillin C (3,5-diprenyl-4-hydroxycinnamic acid) and the derivatives of cinnamic acid besides other flavonoid substances were abundant in the propolis extract used, and they could be the main substances with adjuvant action. The effect of the green propolis extract on the humoral immune response can be exploited in the development of new vaccines.
Fischer
Geferson
G
Centro de Biotecnologia, Universidade Federal de Pelotas (UFPel), Campus Universitário, Caixa Postal 354, 96010-900, Pelotas, RS, Brazil. geferson.fischer@gmail.com
Cleff
Marlete Brum
MB
Dummer
Luana Alves
LA
Paulino
Niraldo
N
Paulino
Amarílis Scremin
AS
de Oliveira Vilela
Camila
C
Campos
Fabrício Souza
FS
Storch
Tiago
T
D'Avila Vargas
Gilberto
G
de Oliveira Hübner
Silvia
S
Vidor
Telmo
T
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D018792:Encephalitis, Viral / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D005260:Female; D006566:Herpesviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D025881:Herpesvirus 5, Bovine / Q000276:immunology*; D022283:Herpesvirus Vaccines / Q000276:immunology* / Q000494:pharmacology; D007114:Immunization / Q000379:methods / Q000662:veterinary; D008297:Male; D008590:Meningoencephalitis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D009500:Neutralization Tests / Q000662:veterinary; D011429:Propolis / Q000276:immunology / Q000494:pharmacology*
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D022283:Herpesvirus Vaccines; D011429:Propolis