In vivo treatment of melanoma (B16F10) with a homeopathic agent and with a cytokine (IFN-alpha).
Among the numerous agents tested on melanoma, cytokines have attracted much attention over recent decades, in particular interferon-alpha (IFN-alpha). However, in a small number of experimental assays, homeopathic products have also been used. This study aimed to analyze the effects of INF-alpha and Lymphomyosot, administered individually or in combination, on the growth of B16F10 melanoma transplanted in C57BL/6J mice. Two experiments were performed using 72 young male mice, treated with 1 x 10(6) B16F10 cells and treated with phosphate-buffered saline (I), INF-alpha (II), Lymphomyosot (III), and both INF-alpha and Lymphomyosot (IV). Subsequent morphological and immunohistochemical studies were performed. All treatments produced a reduction in tumor weight with significant differences in those treated with INF-alpha and Lymphomyosot. INF-alpha reduced the cell proliferation index and the spread of inflammatory infiltrates and produced an increase in the extent of intratumoral necrosis. An antitumour effect was displayed by both agents, as was the cytotoxicity of INF-alpha and the immune response-stimulating effect of Lymphomyosot.
Pascual-Carpe
Fernando
F
Department of Pathology, Faculty of Medicine, Murcia University, Espinardo Campus,30100 Murcia, Spain.
Vicente-Ortega
Vicente
V
Campos-Aranda
Matilde
M
Yañez-Gascón
Josefa
J
D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004359:Drug Therapy, Combination; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation / Q000188:drug therapy / Q000473:pathology; D007262:Infusions, Intravenous; D016898:Interferon-alpha / Q000008:administration & dosage* / Q000627:therapeutic use; D008297:Male; D008419:Materia Medica / Q000008:administration & dosage* / Q000627:therapeutic use; D008545:Melanoma / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D009336:Necrosis / Q000188:drug therapy / Q000473:pathology; D009374:Neoplasms, Experimental / Q000473:pathology; D010936:Plant Extracts / Q000008:administration & dosage* / Q000627:therapeutic use; D016896:Treatment Outcome; D023041:Xenograft Model Antitumor Assays
D016898:Interferon-alpha; C518246:Lymphomyosot; D008419:Materia Medica; D010936:Plant Extracts