title: Characterization of Japanese flounder karyotype by chromosome bandings and fluorescence in situ hybridization with DNA markers.
abstract: The chromosomes of Japanese flounder, Paralichthys olivaceus, were examined by conventional differential staining methods including G-, Q-, C-, silver (Ag)-, fluorochrome, and replication R-bandings and by fluorescence in situ hybridization (FISH) with 5S and 18S rDNAs and telomeric DNA as probes. Replication R-banding substantially made it possible to identify 24 homologous pairs by their RBG-banding pattern and relative length. Both rDNA loci were mapped to chromosome 1, where 5S and 18S rDNA loci were located at the centromeric region and secondary constriction, respectively. C-banding revealed that both rDNA loci were heterochromatic, and 18S rDNA loci were positive for chromomycin A(3) but negative for 4',6-diamidino-2-phenylindole (DAPI) staining. Telomeric FISH signals were observed at all chromosome ends and at the interstitial region of some chromosomes. The observed results were discussed in relation to the karyotype evolution in the order Pleuronectiformes.
authors.affiliation: Inland Station, Fisheries Research Agency, National Research Institute of Aquaculture, Tamaki Mie 519-0423, Japan. jiwara@fra.affrc.go.jp
authors.forename: Atushi
authors.initials: A
authors.lastname: Fujiwara
authors.forename: Mika
authors.initials: M
authors.lastname: Fujiwara
authors.forename: Chizuko
authors.initials: C
authors.lastname: Nishida-Umehara
authors.forename: Syuiti
authors.initials: S
authors.lastname: Abe
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Masaoka
mesh_terms: D000818:Animals; D002871:Chromosome Banding; D002874:Chromosome Mapping; D020732:Cytogenetic Analysis / Q000379:methods*; D004275:DNA, Ribosomal; D019143:Evolution, Molecular; D005432:Flounder / Q000235:genetics*; D005819:Genetic Markers; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping / Q000379:methods*; D013194:Staining and Labeling; D016615:Telomere
chemical_list: D004275:DNA, Ribosomal; D005819:Genetic Markers