title: Prevalence of Rickettsia felis DNA in the blood of cats and their fleas in the United States.
abstract: Rickettsia felis is associated with fever, headache, myalgia, and macular rash in some infected humans and has been detected in the cat flea (Ctenocephalides felis) in many countries around the world. While some naturally exposed cats have been assessed for antibodies against R felis, to our knowledge, no one has reported use of polymerase chain reaction (PCR) to attempt to amplify R felis DNA from client-owned cats and the fleas collected from them. In this study, we assayed 92 pairs of cat blood and flea extracts from Alabama, Maryland and Texas, using PCR assays that amplify a region of the citrate synthase gene (gltA) and the outer membrane protein B gene (ompB). Of the 92 pairs, 62 of 92 (67.4%) flea extracts and none of the cat blood samples were positive for R felis DNA.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA. jrhawley@lamar.colostate.edu
authors.forename: Jennifer R
authors.initials: JR
authors.lastname: Hawley
authors.forename: Susan E
authors.initials: SE
authors.lastname: Shaw
authors.forename: Michael R
authors.initials: MR
authors.lastname: Lappin
mesh_terms: D000407:Alabama / Q000453:epidemiology; D000818:Animals; D002371:Cat Diseases / Q000097:blood / Q000453:epidemiology / Q000382:microbiology* / Q000469:parasitology; D002415:Cats / Q000097:blood* / Q000382:microbiology; D005260:Female; D008297:Male; D008396:Maryland / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D012282:Rickettsia Infections / Q000097:blood / Q000453:epidemiology* / Q000662:veterinary*; D041161:Rickettsia felis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D005423:Siphonaptera / Q000382:microbiology*; D013781:Texas / Q000453:epidemiology