Effects of mannosylated glycopolymers on specific interaction to bone marrow hematopoietic and progenitor cells derived from murine species.
Poly[N-pvinylbenzyl-O-D-galactopyranosyl-(1-4)-D-glucoamide], poly[N-pvinylbenzyl-O-D-glucopyranosyl-(1-4)-D-glucoamide], and poly[N-p-vinylbenzyl-O-mannopyranosyl-(1-4)-D-gluconamide] (referred to as PVLA, PVMA, and PV-Man) are polystyrene derivatives that contain galactose, glucose, and mannose moieties, which interact with hematopoietic cells (HCs). To clarify the specific interaction between the glucopolymers and hematopoietic cells, glycopolymers labeled with fluorescent isothiocyanate (FITC) were used to follow the specific interaction, which was visualized by confocal laser microscopy. We found that PV-Man binds strongly to HCs, probably because of a specific interaction mediated by specific receptors present on the cell membrane, while some cytotoxicity when was observed when PV-Man interacted with the cell membrane. The fluorescence intensity between PV-Man and HCs was up to four-fold (0.14 +/- 0.04) that of PVMA and PVLA with hematopoietic HCs (0.033 +/- 0.01). Moreover, cellular fluorescence increased significantly with increasing incubation time and increasing polymer concentration. Using hematopoietic lineage-specific antibodies, cells were stained and analyzed by flow cytometry to confirm which HCs showed specific binding with glycopolymers, especially hematopoietic stem cells and progenitor cells (c-kit+), B-lymphocyte progenitor cells (B220+), monocyte cells (CD11b+), and erythrocytes (Ter119+).
Park
Keun-Hong
KH
College of Medicine, CHA Stem Cell Institute 606-16, Pochon CHA University, Yeoksam 1-dong, Kangnam-gu, Seoul 135-081, Republic of Korea.
Na
Kun
K
Lee
Yong Su
YS
Chang
Won-Kyong
WK
Park
Jin-Ki
JK
Akaike
Thoshihiro
T
Kim
Dong Ku
DK
D000818:Animals; D001665:Binding Sites; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000378:metabolism*; D066298:In Vitro Techniques; D007785:Lactose / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D008362:Mannosides / Q000737:chemistry; D008422:Materials Testing; D051379:Mice; D018613:Microscopy, Confocal; D011137:Polystyrenes / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*
D001672:Biocompatible Materials; D005456:Fluorescent Dyes; D008362:Mannosides; D011137:Polystyrenes; C074112:poly-N-4-vinylbenzyllactonamide; D016650:Fluorescein-5-isothiocyanate; D007785:Lactose