title: [Bacteriocinogenic highly antagonistic strains of lactobacilli].
abstract: Ability to secrete bacteriocines and microcines was studied in 25 cultures of lactobacilli isolated from intestine of healthy children. Sixteen (64%) of them produced microcines with wide spectrum of antagonistic activity. Susceptibility of microcines-secreting cultures to antibacterial preparations, different dilutions of hydrochloric acid and bile was studied along with their acid-producing ability. Five strains without DNA-se, RNA-se, gelatinase, lecitinase and caseinolytic activity were selected from Sixteen microcines-producing cultures. Three of the selected strains carried plasmid DNA and two didn't have plasmids. Bacteria were characterized by tolerance to hydrochloric acid and bile - minimal inhibitory concentrations for them were 1,25 and 10% respectively. Strains without plasmids were susceptible to majority of wide-spectrum antibiotics and resistant to fluorochinolones. Microcines-producing lactobacilli with wide spectrum of antagonistic activity against pathogenic and conditionally pathogenic microorganisms and tolerance to physiological concentrations of hydrochloric acid and bile have a potential to be used in manufacturing of probiotics.
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Chervinets
authors.forename: V M
authors.initials: VM
authors.lastname: Bondarenko
authors.forename: N A
authors.initials: NA
authors.lastname: Shabanova
authors.forename: A M
authors.initials: AM
authors.lastname: Samoukina
authors.forename: V M
authors.initials: VM
authors.lastname: Chervinets
mesh_terms: D000143:Acids / Q000378:metabolism; D000898:Antibiosis; D003086:Bacteriocin Plasmids / Q000032:analysis; D001430:Bacteriocins / Q000378:metabolism* / Q000494:pharmacology*; D001646:Bile; D002648:Child; D005260:Female; D006801:Humans; D006851:Hydrochloric Acid / Q000494:pharmacology; D007422:Intestines / Q000382:microbiology; D007778:Lactobacillus / Q000187:drug effects / Q000302:isolation & purification / Q000502:physiology*; D008297:Male; D008826:Microbial Sensitivity Tests
chemical_list: D000143:Acids; D001430:Bacteriocins; D006851:Hydrochloric Acid