title: On-line monitoring of changes in host cell physiology during the one-step growth cycle of Bacillus phage Bam35.
abstract: In this study an on-line electrochemical method was developed to examine the one-step growth cycle (OSGC) of the bacteriophage Bam35. The on-line conditions for monitoring the OSGC and the effect of aeration on the duration of the OSGC were defined. The data indicate that binding of phenyldicarbaundecaborane anions to Bacillus thuringiensis cells infected with Bam35 can be used as a sensitive indicator of cell lysis.
authors.affiliation: Department of Biological and Environmental Sciences and Institute of Biotechnology, University of Helsinki, Finland.
authors.forename: Rimantas
authors.initials: R
authors.lastname: Daugelavicius
authors.forename: Ausra
authors.initials: A
authors.lastname: Gaidelyte
authors.forename: Virginija
authors.initials: V
authors.lastname: Cvirkaite-Krupovic
authors.forename: Dennis H
authors.initials: DH
authors.lastname: Bamford
mesh_terms: D017103:Bacillus Phages / Q000254:growth & development*; D001413:Bacillus thuringiensis / Q000378:metabolism / Q000821:virology*; D001896:Boron Compounds / Q000378:metabolism; D018929:Cell Culture Techniques; D008242:Lysogeny / Q000502:physiology*; D013997:Time Factors
chemical_list: D001896:Boron Compounds; C100079:phenyldicarbaundecaborane