title: Diverse sugar-binding specificities of marine invertebrate C-type lectins.
abstract: The sugar-binding specificities of C-type lectins isolated from marine invertebrates were investigated by frontal affinity chromatography (FAC) using 100 oligosaccharides. The lectins included BRA-2 and BRA-3, multiple lectins from the hemolymph of the acorn barnacle, Megabalanus rosa, and BRL from the acorn barnacle, Balanus rostatus. The diverse sugar-binding specificities of the C-type lectins were determined by FAC analysis. BRA-2 recognized alpha2-6 sialylation but not alpha2-3 sialylation on glycans. On the other hand, BRA-3 showed high affinity for oligosaccharides with alpha-linked non-reducing terminal galactose, but not for sialylated forms, and BRL showed enhanced recognition activity towards Lewis(x) and Lewis(a) epitopes.
authors.affiliation: Department of Biomolecular Science, Graduate School of Life Sciences, Tohoku University, Sendai, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Matsubara
authors.forename: Sachiko
authors.initials: S
authors.lastname: Nakamura-Tsuruta
authors.forename: Jun
authors.initials: J
authors.lastname: Hirabayashi
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Jimbo
authors.forename: Hisao
authors.initials: H
authors.lastname: Kamiya
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Ogawa
authors.forename: Koji
authors.initials: K
authors.lastname: Muramoto
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry*; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D007700:Kinetics; D037102:Lectins / Q000737:chemistry*; D037181:Lectins, C-Type / Q000737:chemistry*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry; D001468:Thoracica / Q000737:chemistry*
chemical_list: C519433:BRA-2 lectin, Megabalanus rosa; C519432:BRA-3 lectin, Megabalanus rosa; D002241:Carbohydrates; D037102:Lectins; D037181:Lectins, C-Type; D009844:Oligosaccharides; D011134:Polysaccharides