title: Yeast populations residing on healthy or botrytis-infected grapes from a vineyard in Attica, Greece.
abstract: The yeast flora associated with healthy and Botrytis-infected grapes was assessed. Molecular identification methods assigned isolates to six genera and nine species. For the first time Hanseniaspora opuntiae was encountered as an inhabitant of the grape ecosystem. By using DraI, an informative restriction fragment length polymorphism pattern was generated to distinguish H. opuntiae from the closely related organism Hanseniaspora guilliermondii. Botrytis infection resulted in a larger population and greater diversity of yeasts enriched with fermentative or spoilage species.
authors.affiliation: Laboratory of Microbiology and Biotechnology of Foods, Department of Food Science and Technology, Agricultural University of Athens, Iera Odos 75, Athens, Greece.
authors.forename: Aspasia A
authors.initials: AA
authors.lastname: Nisiotou
authors.forename: George-John E
authors.initials: GJ
authors.lastname: Nychas
mesh_terms: D044822:Biodiversity; D020171:Botrytis / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D006115:Greece; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D027843:Vitis / Q000382:microbiology*; D015003:Yeasts / Q000145:classification* / Q000302:isolation & purification*
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D015252:Deoxyribonucleases, Type II Site-Specific; C035594:TTTAAA -specific type II deoxyribonucleases