title: Diffusion and segmental dynamics of double-stranded DNA.
abstract: Diffusion and segmental dynamics of the double-stranded lambda-phage DNA polymer are quantitatively studied over the transition range from stiff to semiflexible chains. Spectroscopy of fluorescence fluctuations of single-end fluorescently labeled monodisperse DNA fragments unambiguously shows that double-stranded DNA in the length range of 10(2) - 2 x 10(4) base pairs behaves as a semiflexible polymer with segmental dynamics controlled by hydrodynamic interactions.
authors.affiliation: Institute of Biophysics/BIOTEC, Dresden University of Technology, Tatzberg 47-51, 01307 Dresden, Germany.
authors.forename: E P
authors.initials: EP
authors.lastname: Petrov
authors.forename: T
authors.initials: T
authors.lastname: Ohrt
authors.forename: R G
authors.initials: RG
authors.lastname: Winkler
authors.forename: P
authors.initials: P
authors.lastname: Schwille
mesh_terms: D010582:Bacteriophage lambda / Q000378:metabolism*; D004247:DNA / Q000737:chemistry*; D004279:DNA, Viral / Q000737:chemistry*; D004058:Diffusion; D005456:Fluorescent Dyes / Q000737:chemistry; D013057:Spectrum Analysis
chemical_list: D004279:DNA, Viral; D005456:Fluorescent Dyes; D004247:DNA