[A preliminary study of an inherited macrothrombocytopenia disorder with abnormal large granules].
OBJECTIVE
To study the platelet morphology and function of an inherited macrothrombocytopenia disorder with abnormal large granules.


METHODS
Platelet size and structure were investigated by both light microscopy and electron microscopy. The platelet membrane expression of GP I b, GP II b, GPIII a, P-selectin and CD63 were analyzed by using respective monoclonal antibodies. Platelet 5-hydroxy-tryptamine was measured with spectrophotofluorometer.


RESULTS
Both the patient and her father had large granules in their platelets, with exocytosis being easily observed. The expressions of GP I b, GP II b and GP II a on the platelets were in normal range, while P-selectin and CD63 were somewhat increased. The abnormal large granules were not the alpha granules, lysosomes or dense bodies.


CONCLUSIONS
Both morphological and functional abnormalities of the platelets from the patient are clearly distinguishable from other hereditary giant platelet disorders. It would probably represent a novel platelet disorder.
Wu
Shu-Yan
SY
Jiangsu Institute of Hematology, The First Affiliated Hospital of Suzhou University, Suzhou 215006, China.
Wang
Zhao-Yue
ZY
Dai
Lan
L
Huang
Rui
R
Wang
Xiang-Ying
XY
Li
Su-An
SA
Mao
Di-Hua
DH
Ruan
Chang-Geng
CG
D000328:Adult; D001792:Blood Platelets / Q000378:metabolism / Q000648:ultrastructure*; D005260:Female; D006801:Humans; D039661:Integrin beta3 / Q000096:biosynthesis; D016253:Microscopy, Immunoelectron; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000096:biosynthesis; D040201:Platelet Membrane Glycoprotein IIb / Q000096:biosynthesis; D013921:Thrombocytopenia / Q000235:genetics / Q000473:pathology*
D039661:Integrin beta3; D019038:Platelet Glycoprotein GPIb-IX Complex; D040201:Platelet Membrane Glycoprotein IIb