Impact of neonatal hypothyroidism on reproduction in the male hamster, Mesocricetus auratus.
Thyroid hormones play an important role in brain development. In the present study, we examined the influence of transient postnatal hypothyroidism on reproductive neuroendocrine and behavioral outcomes in the male Syrian (golden) hamster. Hamster pups were rendered hypothyroid following exposure to the goitrogen, 6-n-propyl-2-thiouracil (PTU), between postnatal (PN) day 0 (birth) and PN25 (weaning). By 15 days after cessation of PTU, exposure (PN40) serum thyroxine levels had returned to control levels. The testes of treated males were approximately 30% heavier than controls and daily sperm production was increased by 73%. Immunocytochemistry for GnRH revealed that the total number of GnRH neurons did not vary between groups; however, a shift in the distribution of GnRH neurons was observed in treated males such that more GnRH immunoreactive neurons were found in the caudal portion of their normal distribution. The shift in GnRH distribution was associated with a significant reduction (40-50%) in pituitary gonadotropin secretion. Behaviorally, treated males took significantly longer to investigate the anogenital region and then mount a receptive female. A corresponding reduction in the total number of anogenital investigations and mounts was observed. This difference between treated males and controls was reduced, but not eliminated, over successive trials and by the third trial the number of intromission was similar between treated and control males. We conclude that the full complement of adult reproductive functions observed in the male golden hamster requires thyroid hormones during the early postnatal period. The severity of the effects induced by early hypothyroidism in this species varies from transient to permanent, depending on the endpoint.
Jansen
Heiko T
HT
Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, Washington State University, 205 Wegner Hall Pullman, WA 99164-6520, United States. heiko@vetmed.wsu.edu
Kirby
John D
JD
Cooke
Paul S
PS
Arambepola
Niromi
N
Iwamoto
Gary A
GA
D000367:Age Factors; D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D000963:Antimetabolites; D001921:Brain / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D006224:Cricetinae; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D007037:Hypothyroidism / Q000139:chemically induced / Q000503:physiopathology*; D007150:Immunohistochemistry; D008297:Male; D008647:Mesocricetus; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D009490:Neurosecretory Systems / Q000254:growth & development / Q000502:physiology; D009929:Organ Size; D011441:Propylthiouracil; D012098:Reproduction / Q000502:physiology*; D012726:Sexual Behavior, Animal / Q000502:physiology; D013737:Testis / Q000033:anatomy & histology / Q000502:physiology*; D013974:Thyroxine / Q000097:blood
D000963:Antimetabolites; D007987:Gonadotropin-Releasing Hormone; D011441:Propylthiouracil; D013974:Thyroxine