[Expression of minichromosome maintenance protein 6 in craniopharyngioma and its correlation with prognosis].
OBJECTIVE
To investigate the expression of minichromosome maintenance protein 6 (MCM6) in tissue sections of craniopharyngioma and observe its relation with the outcome of patients with craniopharyngiomas.


METHODS
Prospective cohorts were composed of 32 adamantine epithelioma (AE) patients and 31 squamous papillary tumor (SP) patients. The average of follow-up phase was 84. 26 months, of 60 patients with craniopharyngioma, 20 suffered from recurrence and underwent operation again for removal of tumor, and the specimens of the tumors patients were collected. MCM6 as proliferative marker expression in the specimen sections was measured by immunohistochemical method (avidin-biotin-peroxidase); quantitatively, scoring for MCM6 protein variation was performed by TE2000-U inverted biological microscope and Image-Pro Plus professional image analysis software. Oncocyte proliferation potential was evaluated for inter-group comparison in three pair of groups, including AE/SP, recurrence/recurrence-free, and primary/relapse groups.


RESULTS
14 of 32 AE patients and 6 of 31 SP patients had recurrence during follow-up. MCM6 protein expression showed significant difference between AE/SP groups and between recurrence/recurrence-free groups (P < 0.05, two-tailed), but there was no statistically significant difference between primary and recurrent craniopharyngiomas.


CONCLUSIONS
The subtype and MCM6 protein expression in craniopharyngiomas are related to the prognosis of tumor and thus may be useful in predicting the risk of tumor relapse.
Xu
Jian-guo
JG
Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, China.
You
Chao
C
Wang
Xiao-jie
XJ
Shuai
Ke-guang
KG
Wang
Xiao-shu
XS
D000818:Animals; D018797:Cell Cycle Proteins / Q000378:metabolism*; D003397:Craniopharyngioma / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D064151:Minichromosome Maintenance Complex Component 6; D011379:Prognosis; D012008:Recurrence
D018797:Cell Cycle Proteins; C103258:MCM6 protein, human; D064151:Minichromosome Maintenance Complex Component 6