[Effect of Fuzheng Jiangan formula on liver fibrosis induced by albumin in rats].
OBJECTIVE
To observe the effect of Fuzheng Jiangan formula( FZJGF) on liver fibrosis using immune induced liver fibrosis rat model.


METHODS
The rat models with immunity liver fibrosis were induced by the human serum albumin. Rats were treated with normal saline, FZJGF (9. 85,39. 4 g x kg(-1) , two dosage groups) and Colchicine (0. 000 1 g . kg(-1) ). The activities of ALT, AST, contents of ALB and TP, and A/G, The contents of Laminin (LN), Hyaluronic acid (HA) and collagen type IV (IV-C) in rat serum were measured by radioimmunoassay method. The level of hydroxyproline (Hyp) in liver was detected by chemistry method. The pathological changes of liver tissue were observed by HE and Von-Gieson staining.


RESULTS
FZJGF could significant decrease the serum activities of ALT and AST, and increase the levels of TP,Alb and ratio A/G. The levels of LN, HA and IV-C were decreased significantly after the treatment using FZJGF. The pathological improvements were observed. FZJGF could markedly alleviate the deposition of collageneous fiber, and reduce the liver pseudoluboli and the fibrosis scores in the liver tissue compared with model group.


CONCLUSIONS
FZJGF can inhibit formation and development of rat hepatic fibrosis induced by the human serum albumin.
Huang
Xiang-an
XA
Department of Infectious Diseases, Dong Fang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China. hxiangan@163.com
Sun
Li-hong
LH
Song
Chong-shun
CS
Cui
Ning
N
Chen
Yan-li
YL
Xu
Hong-yan
HY
Ren
Ying
Y
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D024141:Collagen Type IV / Q000097:blood; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D006820:Hyaluronic Acid / Q000097:blood; D006909:Hydroxyproline / Q000378:metabolism; D007797:Laminin / Q000097:blood; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008106:Liver Cirrhosis, Experimental / Q000097:blood / Q000139:chemically induced / Q000517:prevention & control*; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012709:Serum Albumin
D024141:Collagen Type IV; D004365:Drugs, Chinese Herbal; D007797:Laminin; D012709:Serum Albumin; C528249:fuzheng jiangan; D006820:Hyaluronic Acid; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D006909:Hydroxyproline