title: Sensory cell proliferation within the olfactory epithelium of developing adult Manduca sexta (Lepidoptera).
abstract: BACKGROUND
  Insects detect a multitude of odors using a broad array of phenotypically distinct olfactory organs referred to as olfactory sensilla. Each sensillum contains one to several sensory neurons and at least three support cells; these cells arise from mitotic activities from one or a small group of defined precursor cells. Sensilla phenotypes are defined by distinct morphologies, and specificities to specific odors; these are the consequence of developmental programs expressed by associated neurons and support cells, and by selection and expression of subpopulations of olfactory genes encoding such proteins as odor receptors, odorant binding proteins, and odor degrading enzymes.
  
  
  RESULTS
  We are investigating development of the olfactory epithelium of adult M. sexta, identifying events which might establish sensilla phenotypes. In the present study, antennal tissue was examined during the first three days of an 18 day development, a period when sensory mitotic activity was previously reported to occur. Each antenna develops as a cylinder with an outward facing sensory epithelium divided into approximately 80 repeat units or annuli. Mitotic proliferation of sensory cells initiated about 20-24 hrs after pupation (a.p.), in pre-existing zones of high density cells lining the proximal and distal borders of each annulus. These high density zones were observed as early as two hr. a.p., and expanded with mitotic activity to fill the mid-annular regions by about 72 hrs a.p. Mitotic activity initiated at a low rate, increasing dramatically after 40-48 hrs a.p.; this activity was enhanced by ecdysteroids, but did not occur in animals entering pupal diapause (which is also ecdysteroid sensitive).
  
  
  CONCLUSIONS
  Sensory proliferation initiates in narrow zones along the proximal and distal borders of each annulus; these zones rapidly expand to fill the mid-annular regions. These zones exist prior to any mitotic activity as regions of high density cells which form either at or prior to pupation. Mitotic sensitivity to ecdysteroids may be a regulatory mechanism coordinating olfactory development with the developmental choice of diapause entry.
authors.affiliation: Department of Biological Sciences, University of South Carolina, Columbia, South Carolina, United States of America.
authors.forename: Marie-Dominique
authors.initials: MD
authors.lastname: Franco
authors.forename: Jonathan
authors.initials: J
authors.lastname: Bohbot
authors.forename: Kenny
authors.initials: K
authors.lastname: Fernandez
authors.forename: Jayd
authors.initials: J
authors.lastname: Hanna
authors.forename: James
authors.initials: J
authors.lastname: Poppy
authors.forename: Richard
authors.initials: R
authors.lastname: Vogt
mesh_terms: D000107:Acetylation; D000825:Animal Structures / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004261:DNA Replication; D004441:Ecdysterone / Q000494:pharmacology; D005260:Female; D006657:Histones / Q000737:chemistry; D006834:Hydrazines / Q000494:pharmacology; D019476:Insect Proteins / Q000737:chemistry; D007814:Larva; D008297:Male; D018402:Manduca / Q000166:cytology* / Q000254:growth & development; D008938:Mitosis / Q000187:drug effects; D009831:Olfactory Mucosa / Q000166:cytology*; D018034:Olfactory Receptor Neurons / Q000166:cytology* / Q000187:drug effects; D011499:Protein Processing, Post-Translational; D011679:Pupa; D012679:Sense Organs / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development
chemical_list: D006657:Histones; D006834:Hydrazines; D019476:Insect Proteins; D004441:Ecdysterone; C082026:tebufenozide