Transformation and fate of 2,4,6-trinitrotoluene (TNT) in anaerobic bioslurry reactors under various aeration schemes: implications for the decontamination of soils.
Energetic compounds have been used in a variety of industrial and military applications worldwide leading to widespread environmental contamination. Many of these compounds are toxic and resist degradation by oxidative enzymes resulting in a need for alternative remediation methods. It has been shown that trinitrotoluene (TNT)-contaminated soil subjected to treatment in strictly anaerobic bioreactors results in tight binding of TNT transformation products to soil organic matter. The research presented here examined the fate of TNT and its metabolites in bioreactors under three different aeration regimes. In all treatment regimes, the typical metabolites of aminodinitrotoluenes and diaminonitrotoluenes were observed prior to irreversible binding into the soil fraction of the slurry. Significant transformation of TNT into organic acids or simple diols, as others report in prior work, was not observed in any of the treatments and is an unlikely fate of TNT in anaerobic soil slurries. These results indicate that aeration does not dramatically affect transformation or fate of TNT in reactor systems that receive a rich carbon source but does affect the rate at which metabolites become tightly bound to the soil. The most rapid transformations and lowest redox potentials were observed in reactors in which an aerobic headspace was maintained suggesting that aerobes play a role in establishing conditions that are most conducive to TNT reduction.
Newcombe
David A
DA
Environmental Biotechnology Institute, University of Idaho, Food Research Center 202, PO Box 441052, Moscow, ID 83844-1052, USA.
Crawford
Ronald L
RL
D000693:Anaerobiosis; D001673:Biodegradation, Environmental; D019149:Bioreactors; D002247:Carbon Isotopes; D003666:Decontamination / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D010084:Oxidation-Reduction; D012722:Sewage / Q000382:microbiology*; D012989:Soil Pollutants / Q000302:isolation & purification*; D013997:Time Factors; D014303:Trinitrotoluene / Q000378:metabolism*
D002247:Carbon Isotopes; D012722:Sewage; D012989:Soil Pollutants; D014303:Trinitrotoluene