title: Somatic mutations of the beta-TrCP gene in gastric cancer.
abstract: Beta-TrCP is a component of the ubiquitin ligase complex targeting beta-catenin for proteasomal degradation, and is a negative regulator of Wnt/beta-catenin signaling. To determine whether genetic alterations of the beta-TrCP gene are involved in the development or progression of gastric cancer, we analyzed its somatic mutations in 95 gastric cancers by single-strand conformational polymorphism and sequencing. We found five missense mutations (5.3%): A99V, H342Y, H425Y, C206Y, and G260E. Tissue carrying mutations showed moderate to strong cytoplasmic and/or nuclear staining of beta-catenin by immunohistochemistry. Thus, somatic mutations of the beta-TrCP gene may contribute to the development of gastric cancer through beta-catenin stabilization.
authors.affiliation: Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors.forename: Chang Jae
authors.initials: CJ
authors.lastname: Kim
authors.forename: Jae Hwi
authors.initials: JH
authors.lastname: Song
authors.forename: Yong Gu
authors.initials: YG
authors.lastname: Cho
authors.forename: Young Sill
authors.initials: YS
authors.lastname: Kim
authors.forename: Su Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Suk Woo
authors.initials: SW
authors.lastname: Nam
authors.forename: Nam Jin
authors.initials: NJ
authors.lastname: Yoo
authors.forename: Jung Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Won Sang
authors.initials: WS
authors.lastname: Park
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D009154:Mutation; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D051176:beta Catenin / Q000378:metabolism; D044844:beta-Transducin Repeat-Containing Proteins / Q000235:genetics*
chemical_list: D017931:DNA Primers; D004273:DNA, Neoplasm; D051176:beta Catenin; D044844:beta-Transducin Repeat-Containing Proteins