Stimulatory effect of phytosulfokine-alpha on the proliferation of tobacco 'Bright Yellow 2' suspension cells.
'Bright Yellow 2' ('BY-2') tobacco (Nicotiana tabacum L.) suspension cells could not proliferate even with proper 2, 4-D concentration (0.6 mg/L) in the medium, when the initial cell density is low. However, the cells could divide and grow normally if conditioned medium (CM) was added to the medium, and the rate of proliferation of cells was proportional to the quantities of CM supplied. The same results were obtained, when the CM was replaced by synthesized phytosulfokine-alpha (PSK-alpha), a sulfated pentapeptide, PSK-alpha was found in CM of 'BY-2' cells by MS identification. From the significant linear relationship between rate of cell proliferation (measured by OD600 value) and concentrations (0.05 nmol/L-10 micromol/L) of PSK-alpha, it can be seen that the 'BY-2' suspended cells are the ideal plant material for bioassay of PSK-alpha. This result suggests that the PSK-alpha might be involved in promoting the proliferation of 'BY-2' suspension cells.
Chen
Jun-Ying
JY
College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China.
Yang
Yan-Hui
YH
Xu
Hai-Xia
HX
Cheng
Xi-Yong
XY
Chen
Xin-Jian
XJ
D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D036361:Peptide Hormones; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000138:chemical synthesis / Q000494:pharmacology*; D013535:Suspensions; D014026:Nicotiana / Q000166:cytology*
D017077:Culture Media, Conditioned; C100975:PSK-alpha protein, plant; D036361:Peptide Hormones; D010937:Plant Growth Regulators; D010940:Plant Proteins; D013535:Suspensions