Changes in tissue defence system in white spot syndrome virus (WSSV) infected Penaeus monodon.
The present study examined the changes occurring in the pro phenoloxidase system and antioxidant defence status in haemolymph, hepatopancreas and muscle tissue of white spot syndrome virus (WSSV) infected Penaeus monodon. Tiger shrimps (P. monodon) were infected with white spot virus by intramuscular injection of the virus inoculum. Levels of lipid peroxides and the activities of phenoloxidase, glutathione-dependent antioxidant enzymes [glutathione peroxidase (GPX), glutathione-S-transferase (GST)] and antiperoxidative enzymes [superoxide dismutase (SOD) and catalase (CAT)] were determined. WSSV infection induced a significant increase in lipid peroxidation in haemolymph, muscle and hepatopancreas of experimental P. monodon compared to normal controls. This was paralleled by significant reduction in the activities of phenol oxidase, glutathione-dependent antioxidant enzymes and antiperoxidative enzymes. The results of the present study indicate that the tissue antioxidant defence system in WSSV infected P. monodon is operating at a lower rate, which ultimately resulted in the failure of counteraction of free radicals, leading to oxidative stress as evidenced by the increased level of lipid peroxidation.
Mathew
Suseela
S
Biochemistry and Nutrition Division, Central Institute of Fisheries Technology, Matsyapuri PO, Cochin 682 029, Kerala, India. suseela@ciftmail.org
Kumar
K Ashok
KA
Anandan
R
R
Viswanathan Nair
P G
PG
Devadasan
K
K
D000818:Animals; D002374:Catalase / Q000378:metabolism; D015227:Lipid Peroxidation; D014442:Monophenol Monooxygenase / Q000378:metabolism; D033561:Penaeidae / Q000276:immunology / Q000378:metabolism / Q000821:virology*; D013482:Superoxide Dismutase / Q000378:metabolism; D046848:White spot syndrome virus 1 / Q000502:physiology*
D002374:Catalase; D014442:Monophenol Monooxygenase; D013482:Superoxide Dismutase