Histological study of the liver and biochemistry of the blood of Wistar rats following ligature of right hepatic duct.
OBJECTIVE
To observe the histological alterations in the liver and biochemistry in the blood that can happen in Wistar rat, after the ligature of right hepatic duct.


METHODS
In this study were used rats (n=46) of Wistar pedigree. The animal groups (n=46) were distributed in 6 experimented sub-groups (n=6). It was held a ligature surgery of the right hepatic duct and euthanasia in 7, 14, 21, 28, 60 and 90 days and the biochemistry control group (n=10), that animals had 2ml of their blood taken by cardiac puncture for biochemistry study with value analyses of bilirubins, transaminasis, lactic desidrogenasis, alkaline phophatase and gamma-glutamil-transferase. Given the expected time of each group, the animals were submitted to anesthesia procedure and cavity re-opening, being held intra-cardiac puncture and with 2ml blood collected for biochemistry analyses. It was proceeded the liver resection, being the liver put in formol solution to 10% for a period of 24 hours and taken to the histology.


RESULTS
It was not possible to identify results that express significant differences as the existence of alterations histological and biochemical between the different groups.


CONCLUSIONS
At the end of the study, it was not possible to identify histological and biochemical alterations that express significant differences between livers of the animals from the right linked hepatic duct and the animals of the control group.
Quaresma
Abel Botelho
AB
Surgical Technique and Experimental Surgery, Federal University of Santa Catarina, 88024-420 Florianópolis, SC, Brazil.
d'Acampora
Armando José
AJ
Tramonte
Ricardo
R
Farias
Débora Cadore de
DC
Joly
Fabrícia Slomsky
FS
D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001652:Bile Ducts / Q000601:surgery*; D001663:Bilirubin / Q000097:blood; D002779:Cholestasis / Q000097:blood* / Q000209:etiology; D004195:Disease Models, Animal; D005355:Fibrosis; D008026:Ligation; D008099:Liver / Q000473:pathology*; D008297:Male; D011677:Punctures; D051381:Rats; D017208:Rats, Wistar; D018709:Statistics, Nonparametric; D000267:Tissue Adhesions; D000637:Transaminases / Q000097:blood
D000637:Transaminases; D000469:Alkaline Phosphatase; D001663:Bilirubin