title: Influence of macrophage activation on their capacity to bind bacterial antigens studied with atomic force microscopy.
abstract: In this work we studied interactions between bacterial antigens and receptors on the surface of macrophages using atomic force microscopy (AFM). We used two bacterial cell wall components: lipopolysaccharide (LPS) derived from gram-negative Escherichia coli and exopolysaccharide (EPS) derived from gram-positive Lactobacillus rhamnosus. Interactions between these bacterial antigens and immune cell receptors were studied in peritoneal macrophages derived from two strains of mice, CBA and C3H/J, in which the Toll-like receptor 4 (TLR4) is genetically disabled. We collected 500 force-distance curves for LPS-activated cells using an EPS-covered AFM tip, and for EPS-activated cells using an LPS-covered AFM tip. Nonactivated cells were tested as reference cells. The results show that LPS-primed macrophages decrease their ability to bind EPS. Surprisingly, EPS-activated macrophages maintain or even increase their ability to bind LPS. This may suggest that in vivo commensal enteric bacteria, such as lactobacilli, will enhance the defense potential of local macrophages against pathogens expressing LPS.
authors.affiliation: Institute of Physics, Jagiellonian University, Cracow, Poland. mtargosz@if.uj.edu.pl <mtargosz@if.uj.edu.pl>
authors.forename: Marta
authors.initials: M
authors.lastname: Targosz
authors.forename: Aleksander
authors.initials: A
authors.lastname: Labuda
authors.forename: Pawel
authors.initials: P
authors.lastname: Czuba
authors.forename: Rafal
authors.initials: R
authors.lastname: Biedroń
authors.forename: Magdalena
authors.initials: M
authors.lastname: Strus
authors.forename: Andrzej
authors.initials: A
authors.lastname: Gamian
authors.forename: Janusz
authors.initials: J
authors.lastname: Marcinkiewicz
authors.forename: Marek
authors.initials: M
authors.lastname: Szymoński
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology* / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects / Q000276:immunology*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology* / Q000648:ultrastructure*; D051379:Mice; D018625:Microscopy, Atomic Force
chemical_list: D000942:Antigens, Bacterial; D008070:Lipopolysaccharides