title: New fluorescent tools for watching nanometer-scale conformational changes of single molecules.
abstract: Single-molecule biophysics has been serving biology for more than two decades. Fluorescence microscopy is one of the most commonly used tools to identify molecules of interest and to visualize biological events. Here we describe some of the most commonly used fluorescence imaging tools to measure nanoscale movements and the rotational dynamics of biomolecules.
authors.affiliation: Center for Biophysics and Computational Biology, University of Illinois, Urbana-Champaign, Urbana, Illinois 61801, USA. toprak@uiuc.edu
authors.forename: Erdal
authors.initials: E
authors.lastname: Toprak
authors.forename: Paul R
authors.initials: PR
authors.lastname: Selvin
mesh_terms: D000818:Animals; D001703:Biophysics / Q000379:methods*; D002627:Chemistry, Physical / Q000379:methods; D003599:Cytoskeleton / Q000378:metabolism; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008856:Microscopy, Fluorescence; D015233:Models, Statistical; D008968:Molecular Conformation; D024701:Myosin Type V / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D016011:Normal Distribution; D017785:Photons; D013997:Time Factors
chemical_list: D024701:Myosin Type V