Factors influencing success rate of leukocyte immunization and anti-paternal antibodies in spontaneous recurrent miscarriage.
OBJECTIVE
Paternal leukocyte immunotherapy has been used as treatment for spontaneous recurrent miscarriage; however, controversies have raged against the factors influencing its success.


METHODS
Five hundred and forty-nine women with three or more unexplained pregnancy losses were offered immunization. Two weeks after the second immunization, serum was tested for anti-paternal cytotoxic antibody (APCA) by cross matching and if revealed positive she was advised pregnancy. Meanwhile, decision trees have been developed by Waikato Environment for Knowledge Analysis package to predict factors influencing APCA production and pregnancy.


RESULTS
Of 549 subjects, 93 (16.9%) revealed to have positive APCA, among whom 49(52.7%) had clinically successful pregnancy (> or =28 weeks). Patient's age, consanguinity between couples and previous history of surgery, and infectious, endocrine, immunologic, uterine and cervix disorders were inversely associated with APCA production and clinically successful pregnancy, whilst, number of children was a potent positive predictor.


CONCLUSIONS
Leukocyte immunization could be borne in mind as a therapeutic approach in selected subjects. Younger females with negative past medical history who are not frequent aborters are more likely to take benefit from this therapeutic approach if they do not have any familial relationship with their spouse.
Chaichian
Shahla
S
Islamic Azad University, School of Medical Sciences of Tehran, Zargandeh, Dr. Shariati Avenue, Tehran 19168, Iran. drchaichian@yahoo.com
Shoaee
Shervan
S
Saremi
Abutaleb
A
Pedar
Setareh
S
Firouzi
Farzad
F
D000026:Abortion, Habitual / Q000276:immunology / Q000628:therapy*; D000328:Adult; D000906:Antibodies / Q000276:immunology*; D000961:Antilymphocyte Serum / Q000276:immunology; D015331:Cohort Studies; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D006801:Humans; D007114:Immunization; D007492:Iran; D007962:Leukocytes / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D011247:Pregnancy; D011256:Pregnancy Outcome; D012307:Risk Factors; D016896:Treatment Outcome
D000906:Antibodies; D000961:Antilymphocyte Serum