title: Compositae dermatitis from airborne parthenolide.
abstract: BACKGROUND
  Compositae dermatitis confined to exposed skin has often been considered on clinical grounds to be airborne. Although anecdotal clinical and plant chemical reports suggest true airborne allergy, no proof has been procured. Feverfew (Tanacetum parthenium) is a European Compositae plant suspected of causing airborne contact allergy, and its most important allergen is the sesquiterpene lactone (SQL) parthenolide (PHL).
  
  
  OBJECTIVE
  The aims of this study were to (i) assess the allergenicity of feverfew-derived monoterpenes and sesquiterpenes and their oxidized products in feverfew-allergic patients and (ii) re-assess the role of PHL and other SQLs in airborne contact allergy.
  
  
  METHODS
  Feverfew-allergic patients were patch tested with extracts and fractions containing volatile monoterpenes and sesquiterpenes as well as extracts of airborne particles from flowering feverfew plants, obtained by fractionation of ether extracts, dynamic headspace and high-volume air sampler (HIVAS) technique, respectively.
  
  
  RESULTS
  Among 12 feverfew-allergic patients, eight had positive patch-test reactions to a HIVAS filter extract, while two tested positive to a headspace extract. Subsequent analysis of the HIVAS extract by gas chromatography and mass spectrometry detected PHL in a concentration of 510 ng mL(-1) in the HIVAS extract. Testing with a dilution series of PHL showed positive reactions down to 8.1 ng in selected patients. None of the 12 patients tested positive to monoterpenes or sesquiterpenes, whether they were oxidized or not.
  
  
  CONCLUSIONS
  The clinical results have proved that some feverfew-allergic patients are sensitive to airborne particles released from the plant, and isolation of PHL from the particle-containing HIVAS extract in allergenic amounts is strong evidence of PHL as the responsible allergen.
authors.affiliation: Department of Dermatology, Odense University Hospital, DK-5000 Odense C, Denmark. evy.paulsen@ouh.fyns-amt.dk
authors.forename: E
authors.initials: E
authors.lastname: Paulsen
authors.forename: L P
authors.initials: LP
authors.lastname: Christensen
authors.forename: K E
authors.initials: KE
authors.lastname: Andersen
mesh_terms: D000328:Adult; D000368:Aged; D000393:Air Pollutants / Q000032:analysis / Q000276:immunology*; D000485:Allergens / Q000032:analysis / Q000276:immunology; D017449:Dermatitis, Allergic Contact / Q000209:etiology*; D004784:Environmental Monitoring / Q000379:methods; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D008297:Male; D008875:Middle Aged; D010328:Patch Tests; D012717:Sesquiterpenes / Q000032:analysis / Q000276:immunology*; D020903:Tanacetum parthenium / Q000276:immunology*; D014835:Volatilization
chemical_list: D000393:Air Pollutants; D000485:Allergens; D012717:Sesquiterpenes; C002669:parthenolide