title: Supplementation effect of ectoine on thermostability of phytase.
abstract: In this study, we elucidated the supplementation effect of compatible solutes on the thermostability of phytase, designated as PHYA II, which was encoded by the phytase gene phyA I (GeneBank AY013315) from Aspergillus ficuum As3.324 and expressed in Pichia pastoris GS115. When PHYA II in acetate buffer was heated at 90 degrees C for 15 min, more than 80% of the residual activity was retained by adding the cyclic amino acid ectoine, a representative compatible solute. Furthermore, the presence of ectoine led to an increase in the relative hydrolytic rate of sodium phytate by 15.7% with heating at 80 degrees C for 15 min. Among the compatible solutes examined, ectoine was confirmed to be the most efficient thermoprotectant for PHYA II.
authors.affiliation: College of Environmental Science and Engineering, Dalian Maritime University, Lingshui Road, Dalian 116026, P.R. China.
authors.forename: Linghua
authors.initials: L
authors.lastname: Zhang
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.forename: Chunyu
authors.initials: C
authors.lastname: Zhang
authors.forename: Yunji
authors.initials: Y
authors.lastname: Wang
authors.forename: Daochen
authors.initials: D
authors.lastname: Zhu
authors.forename: Chenxiang
authors.initials: C
authors.lastname: Wang
authors.forename: Shinichi
authors.initials: S
authors.lastname: Nagata
mesh_terms: D000599:Amino Acids, Diamino / Q000737:chemistry*; D001230:Aspergillus / Q000737:chemistry* / Q000201:enzymology*; D004795:Enzyme Stability; D006868:Hydrolysis; D011489:Protein Denaturation; D012995:Solubility; D013696:Temperature
chemical_list: D000599:Amino Acids, Diamino; C045628:ectoine