Augmentation of antigen-specific antibody production and IL-10 generation with a fraction from Rooibos (Aspalathus linearis) tea.
Rooibos tea was extracted with boiling water. The aqueous extract was chromatographed in a Diaion HP20 column eluted stepwise with water, 25%, 50% and 75% (v/v) aqueous methanol, and 100% methanol. The water eluate (fraction A) showed an augmenting effect on anti-ovalbumin (anti-OVA) immunoglobulin M (IgM) production in OVA-stimulated murine splenocytes in vitro. Fraction A also showed a strong augmenting effect on interleukin-10 generation in murine splenocytes. Furthermore, continuous ingestion of fraction A was found to increase the anti-OVA IgM level in the sera of OVA-immunized mice.
Ichiyama
Kenji
K
Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University, Okayama, Japan.
Tai
Akihiro
A
Yamamoto
Itaru
I
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D000918:Antibody Specificity / Q000187:drug effects; D036903:Aspalathus / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000235:genetics; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000096:biosynthesis / Q000235:genetics; D010936:Plant Extracts / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013268:Stimulation, Chemical; D018417:Th1 Cells / Q000187:drug effects / Q000276:immunology; D018418:Th2 Cells / Q000187:drug effects / Q000276:immunology
D007075:Immunoglobulin M; D007376:Interleukin-2; D010936:Plant Extracts; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma; D010047:Ovalbumin