An informatic pipeline for the data capture and submission of quantitative proteomic data using iTRAQ.
BACKGROUND
Proteomics continues to play a critical role in post-genomic science as continued advances in mass spectrometry and analytical chemistry support the separation and identification of increasing numbers of peptides and proteins from their characteristic mass spectra. In order to facilitate the sharing of this data, various standard formats have been, and continue to be, developed. Still not fully mature however, these are not yet able to cope with the increasing number of quantitative proteomic technologies that are being developed.


RESULTS
We propose an extension to the PRIDE and mzData XML schema to accommodate the concept of multiple samples per experiment, and in addition, capture the intensities of the iTRAQ reporter ions in the entry. A simple Java-client has been developed to capture and convert the raw data from common spectral file formats, which also uses a third-party open source tool for the generation of iTRAQ reported intensities from Mascot output, into a valid PRIDE XML entry.


CONCLUSIONS
We describe an extension to the PRIDE and mzData schemas to enable the capture of quantitative data. Currently this is limited to iTRAQ data but is readily extensible for other quantitative proteomic technologies. Furthermore, a software tool has been developed which enables conversion from various mass spectrum file formats and corresponding Mascot peptide identifications to PRIDE formatted XML. The tool represents a simple approach to preparing quantitative and qualitative data for submission to repositories such as PRIDE, which is necessary to facilitate data deposition and sharing in public domain database. The software is freely available from http://www.mcisb.org/software/PrideWizard.
Siepen
Jennifer A
JA
Faculty of Life Sciences, University of Manchester, M13 9PT, UK.
Swainston
Neil
N
Manchester Interdisciplinary Biocentre, University of Manchester, UK.
Jones
Andrew R
AR
Faculty of Life Sciences, University of Manchester, M13 9PT, UK.|School of Computer Science, Faculty of Engineering and Physical Sciences, University of Manchester, UK.
Hart
Sarah R
SR
MBCMS, School of Chemistry, Manchester Interdisciplinary Biocentre, University of Manchester, UK.
Hermjakob
Henning
H
EMBL Outstation EBI, Wellcome Trust Genome Campus, Hinxton, Cambs, UK.
Jones
Philip
P
EMBL Outstation EBI, Wellcome Trust Genome Campus, Hinxton, Cambs, UK.
Hubbard
Simon J
SJ
Faculty of Life Sciences, University of Manchester, M13 9PT, UK.