Expression of CYP2S1 in human hepatic stellate cells.
Activated stellate cells are myofibroblast-like cells associated with the generation of fibrotic scaring in chronically damaged liver. Gene chip analysis was performed on cultured fibrotic stellate cells. Of the 51 human CYP genes known, 13 CYP and 5 CYP reduction-related genes were detected with 4 CYPs (CYP1A1, CYP2E1, CY2S1 and CYP4F3) consistently present in stellate cells isolated from three individuals. Quantitative RT-PCR indicated that CYP2S1 was a major expressed CYP mRNA transcript. The presence of a CYP2A-related protein and testosterone metabolism in stellate cell cultures suggest that stellate cells express specific functional isoforms of CYP of which a major form is CYP2S1.
Marek
Carylyn J
CJ
School of Medical Sciences, Institute of Medical Science, University of Aberdeen, Aberdeen, UK.
Tucker
Steven J
SJ
Koruth
Matthew
M
Wallace
Karen
K
Wright
Matthew C
MC
D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism*; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D022781:Hepatocytes / Q000201:enzymology*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System; C422564:CYP2S1 protein, human