title: Metabolic regulation of floral scent in Petunia axillaris lines: biosynthetic relationship between dihydroconiferyl acetate and iso-eugenol.
abstract: Aromatic scent-related compounds in flowers were comprehensively analyzed by high-performance liquid chromatography (HPLC) based on their absorption spectra to understand regulation of metabolism leading to floral scent diversity in Petunia axillaris lines. An unknown compound occurring at similar levels to scent compounds in some plant lines was identified to be dihydroconiferyl acetate. Based on the structure, dihydroconiferyl acetate is likely to be a biosynthetically closely related compound to aromatic scent compounds, especially iso-eugenol. Similar time-course changes of the concentrations suggest that the metabolism of dihydroconiferyl acetate is underlaid by the similar regulation to aromatic scent compounds. Dihydroconiferyl acetate and iso-eugenol occurred almost exclusively, implying that metabolism of the common precursors to each compound is selectively regulated in these plants. The branching of the biosynthetic pathway into dihydroconiferyl acetate and iso-eugenol is probably one of regulatory steps leading to scent diversity in P. axillaris lines.
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Kondo
authors.forename: Naomi
authors.initials: N
authors.lastname: Oyama-Okubo
authors.forename: Masanori
authors.initials: M
authors.lastname: Sagae
authors.forename: Toshio
authors.initials: T
authors.lastname: Ando
authors.forename: Eduardo
authors.initials: E
authors.lastname: Marchesi
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Nakayama
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002940:Circadian Rhythm; D005054:Eugenol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D035264:Flowers / Q000737:chemistry / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D009812:Odorants / Q000032:analysis*; D032306:Petunia / Q000737:chemistry / Q000378:metabolism*; D010636:Phenols / Q000737:chemistry / Q000378:metabolism*; D014835:Volatilization
chemical_list: D010636:Phenols; C106832:dihydroconiferyl alcohol; D005054:Eugenol; C036643:isoeugenol