title: Genetic differences between Culex pipiens f. molestus and Culex pipiens pipiens (Diptera: Culicidae) in New York.
abstract: The definition and phylogenetic placement of the autogenous molestus form of Culex pipiens has puzzled entomologists for decades. We identified genetic differences between Cx. p. pipiens (L.) and Cx. pipiens f. molestus Forskål in the SH60 fragment described previously. Single-strand conformation polymorphism analysis, cloning, and sequencing of this fragment demonstrated high polymorphism within and among individual Cx. p. pipiens, with common SH60 variants shared among individuals from distant locations. In contrast, Cx. pipiens f. molestus from New York City each contained a single SH60 variant, which was not identified in any other Cx. p. pipiens specimens analyzed. Supporting microsatellite analysis demonstrated significant but reduced gene flow between Cx. p. pipiens and Cx. pipiens f. molestus in New York relative to Cx. p. pipiens populations in New York and California. Results are discussed in the context of two contrasting hypotheses regarding the origin of Cx. pipiens f. molestus populations.
authors.affiliation: The W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 N. Wolfe St., Rm E5132, Baltimore, MD 21205, USA. rcmosquito@yahoo.com
authors.forename: Rebekah J
authors.initials: RJ
authors.lastname: Kent
authors.forename: Laura C
authors.initials: LC
authors.lastname: Harrington
authors.forename: Douglas E
authors.initials: DE
authors.lastname: Norris
mesh_terms: D000818:Animals; D001483:Base Sequence; D003465:Culex / Q000235:genetics*; D051456:Gene Flow / Q000235:genetics; D014644:Genetic Variation; D007303:Insect Vectors / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D009518:New York; D016415:Sequence Alignment