Single cell-based analysis of torenia petal pigments by a combination of ArF excimer laser micro sampling and nano-high performance liquid chromatography (HPLC)-mass spectrometry.
The molecular constituents of the petal pigments of the Torenia plant (Torenia hybrida) were analyzed on a single-cell basis by a combination of newly developed laser-microsampling and nano-flow liquid chromatography-electro spray ionization mass spectrometry (LC-ESIMS) techniques. Our method should provide a facile method for obtaining precise metabolic profiles of each cell in a single plant tissue.
Kajiyama
Shin'ichiro
S
Department of Biotechnology, Faculty of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Harada
Kazuo
K
Fukusaki
Eiichiro
E
Kobayashi
Akio
A
D000872:Anthocyanins / Q000378:metabolism*; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D035264:Flowers / Q000378:metabolism; D007834:Lasers; D019684:Magnoliopsida / Q000378:metabolism*; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods; D042282:Microdissection / Q000295:instrumentation* / Q000379:methods; D008846:Micromanipulation / Q000295:instrumentation / Q000379:methods; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods; D010860:Pigments, Biological / Q000378:metabolism; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods
D000872:Anthocyanins; D010860:Pigments, Biological