title: The expression level of luciferase within tumour cells can alter tumour growth upon in vivo bioluminescence imaging.
abstract: In vivo bioluminescence imaging is becoming a very important tool for the study of a variety of cellular and molecular events or disease processes in living systems. In vivo bioluminescence imaging is based on the detection of light emitted from within an animal. The light is generated as a product of the luciferase-luciferin reaction taking place in a cell. In this study, we implanted mice with tumour cells expressing either a high or a low level of luciferase. In vivo bioluminescence imaging was used to follow tumour progression. Repeated luciferin injection and imaging of high and low luciferase-expressing tumours was performed. While low luciferase-expressing tumours grew similarly to vector controls, growth of the high luciferase-expressing tumours was severely inhibited. The observation that a high level of luciferase expression will inhibit tumour cell growth when an animal is subjected to serial in vivo bioluminescence imaging is potentially an important factor in designing these types of studies.
authors.affiliation: Department of Obstetrics and Gynecology, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
authors.forename: Susan
authors.initials: S
authors.lastname: Brutkiewicz
authors.forename: Marc
authors.initials: M
authors.lastname: Mendonca
authors.forename: Keith
authors.initials: K
authors.lastname: Stantz
authors.forename: Kathleen
authors.initials: K
authors.lastname: Comerford
authors.forename: Robert
authors.initials: R
authors.lastname: Bigsby
authors.forename: Gary
authors.initials: G
authors.lastname: Hutchins
authors.forename: Mark
authors.initials: M
authors.lastname: Goebl
authors.forename: Maureen
authors.initials: M
authors.lastname: Harrington
mesh_terms: D000818:Animals; D002455:Cell Division; D005260:Female; D008156:Luciferases / Q000378:metabolism*; D008163:Luminescent Measurements / Q000379:methods*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000201:enzymology* / Q000473:pathology
chemical_list: D008156:Luciferases