title: Surface plasmon resonance biochips for tuberculosis bacillus detection.
abstract: Tuberculosis is one of notifiable infectious diseases which may cause serious epidemic problems. Traditional diagnostic techniques include acid-fast stain, bacteria culture, metabolic monitoring have some disadvantages such as low sensitivity, time consumption and less specificity. In this study we proposed a new diagnostic technique based on the protein chips concept which detected by SPR phenomena. It was found that the resonance angle approximately 51.86 degrees ) shifted slightly toward right with antibody concentration of 10X, 30X, 100X, and 300X dilution under optimal concentration of immobilized TB antigen W38 (MW 41.5 kDa, 50 mug/ml). While for the W06 (MW 14 kDa, 50 mug/ml), the resonance angle was around 50.13 degrees and shifted with the same trend as W38 did.
authors.affiliation: Inst. of Biomed. Eng., Nat. Taiwan Univ., Taipei, Taiwan.
authors.forename: Jhen-Gang
authors.initials: JG
authors.lastname: Huang
authors.forename: Chi-Chan
authors.initials: CC
authors.lastname: Hung
authors.forename: Hsin-Chih
authors.initials: HC
authors.lastname: Lai
authors.forename: Chih-Kung
authors.initials: CK
authors.lastname: Lee
authors.forename: Shi-Ming
authors.initials: SM
authors.lastname: Lin
authors.forename: Ping
authors.initials: P
authors.lastname: Feng
authors.forename: Chii-Wann
authors.initials: CW
authors.lastname: Lin