[The mechanism and significance of cellular signal transduction in kidney glomerular mesangial cells of rat with obstructive jaundice].
OBJECTIVE
To study the mechanism of ceramide-induced cellular signal transduction and its effect on renal injury.


METHODS
Sixty male Sprague-Dawley rats were randomly assigned to four groups (n = 15): control group, sham operation group, experiment group one and experiment group two. Except the control and the sham operation group, the other two experiment groups underwent the common bile duct bound to form the rat model of obstructive jaundice. The renal glomerular mesangial cells (GMC) were cultured primarily. After GMC were given different stimulating factors, we performed the measurement of the PLD activity and the GMC apoptosis analyzed by flow cytometry.


RESULTS
The biological activity of PC-PLD was significantly decreased by TNF-alpha and ceramide (P< 0.05). On the other hand, the GMC apoptosis was induced by TNF-alpha and ceramide (P <0. 05). However, other opposite results were obtained in addition of ceramide inhibitor to GMC.


CONCLUSIONS
TNF-alpha can induce the ceramide increase in GMC, which is significant to effect on the kidney injury of the rats with obstructive jaundice, by the means of inducing GMC apoptosis and decreasing the activity of PC-PLD.
Peng
Bing
B
Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Qiao
An-yi
AY
Luo
Yan-li
YL
Li
Zhi-yao
ZY
He
Sheng
S
Wu
Yan-tao
YT
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D002518:Ceramides / Q000494:pharmacology; D041781:Jaundice, Obstructive / Q000378:metabolism / Q000473:pathology*; D007678:Kidney Glomerulus / Q000187:drug effects / Q000473:pathology*; D008297:Male; D050527:Mesangial Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D002518:Ceramides; D014409:Tumor Necrosis Factor-alpha