The putative glycosyltransferase-encoding gene cylJ and the group B Streptococcus (GBS)-specific gene cylK modulate hemolysin production and virulence of GBS.
Group B streptococcus (GBS) expresses a hemolysin/cytolysin that plays an important role in pathogenesis. Using the Himar1 transposon mutagenesis system, a hypohemolytic mutant carrying an interrupted cylJ gene was characterized. cylJ, encoding a putative glycosyltransferase, and cylK, whose product is unknown, are both required for the full hemolytic/cytolytic activity, pigment formation, and virulence of GBS.
Forquin
Marie-Pierre
MP
Unité de Biologie des Bactéries Pathogènes à Gram-Positif, Institut Pasteur, 25 Rue du Dr. Roux, 75724 Paris Cedex 15, France. sdramsi@pasteur.fr
Tazi
Asmae
A
Rosa-Fraile
Manuel
M
Poyart
Claire
C
Trieu-Cuot
Patrick
P
Dramsi
Shaynoor
S
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D004251:DNA Transposable Elements; D004467:Economics; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D005796:Genes; D005798:Genes, Bacterial; D005964:Glucosyltransferases / Q000235:genetics*; D006460:Hemolysin Proteins / Q000096:biosynthesis*; D016254:Mutagenesis, Insertional; D010860:Pigments, Biological / Q000096:biosynthesis; D051381:Rats; D013290:Streptococcal Infections / Q000382:microbiology; D013291:Streptococcus; D013292:Streptococcus agalactiae / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D014774:Virulence / Q000235:genetics
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D006460:Hemolysin Proteins; D010860:Pigments, Biological; D005964:Glucosyltransferases