title: Local and systemic pathophysiological alterations induced by a serine proteinase from the venom of the snake Bothrops jararacussu.
abstract: The local and systemic pathophysiological alterations induced by BjussuSP-I, a thrombin-like serine proteinase from the venom of the snake Bothrops jararacussu, were assessed in mice. BjussuSP-I induced a mild edema but no local myonecrosis or hemorrhage. It did not induce any microvascular alteration in the cremaster muscle. Intramuscular injection of BjussuSP-I promoted an increase in the expression of proMMP-9, but it did not induce the activation of proMMP-2 or proMMP-9 synthesized in muscle tissue injected with a myotoxic phospholipase A(2) homolog. BjussuSP-I induced defibrin(ogen)ation upon intravenous and intramuscular injections, with reduction in plasma fibrinogen concentration and increments in the levels of fibrin degradation products and D-dimer. When compared with animals having normal coagulation, mice defibrin(ogen)ated by BjussuSP-I developed a slightly larger hemorrhagic lesion in the skin when injected with metalloproteinase BaP1. Intravenous injection of sublethal doses of BjussuSP-I promoted a series of behavioral and motor changes similar to those previously described for 'gyroxin', i.e. opisthotonus and a circular body movement along the longitudinal axis.
authors.affiliation: Facultad de Medicina, Universidad de El Salvador, San Salvador, El Salvador.
authors.forename: Aida Verónica
authors.initials: AV
authors.lastname: Pérez
authors.forename: Patricia
authors.initials: P
authors.lastname: Saravia
authors.forename: Alexandra
authors.initials: A
authors.lastname: Rucavado
authors.forename: Carolina D
authors.initials: CD
authors.lastname: Sant'Ana
authors.forename: Andreimar M
authors.initials: AM
authors.lastname: Soares
authors.forename: José María
authors.initials: JM
authors.lastname: Gutiérrez
mesh_terms: D000818:Animals; D017837:Bothrops; D003435:Crotalid Venoms / Q000201:enzymology*; D004487:Edema / Q000139:chemically induced; D004789:Enzyme Activation / Q000187:drug effects; D004792:Enzyme Precursors / Q000187:drug effects / Q000378:metabolism; D018093:Gelatinases / Q000187:drug effects; D006470:Hemorrhage / Q000139:chemically induced; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D008666:Metalloendopeptidases / Q000187:drug effects; D051379:Mice; D009132:Muscles / Q000473:pathology; D009336:Necrosis / Q000139:chemically induced; D012697:Serine Endopeptidases / Q000302:isolation & purification / Q000633:toxicity*; D018675:Toxicity Tests
chemical_list: D003435:Crotalid Venoms; D004792:Enzyme Precursors; D012697:Serine Endopeptidases; D018093:Gelatinases; D008666:Metalloendopeptidases; C120487:pro-matrix metalloproteinase 9; C069860:progelatinase; D020780:Matrix Metalloproteinase 9