title: Calculation of metabolic flow of xylose in Lactococcus lactis.
abstract: A circuit diagram is proposed on the basis of an analysis of metabolic pathways of lactic acid bacteria, namely, a phosphoketolase pathway and a pentose phosphate/glycolic pathway. An augmented matrix was derived from carbon balances and stoichiometries from the circuit diagram, and solved by Gaussian elimination. Results indicated that 70% of the amount of xylose was metabolized through the pentose phosphate/glycolic pathway and that the yield of lactic acid from xylose ranged from 0.34 to 0.52 (mol/mol), whereas that of pyruvic acid was more than 1.4 (mol/mol).
authors.affiliation: R and D Center for Bio-based Materials, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan. ohara@kit.jp
authors.forename: Hitomi
authors.initials: H
authors.lastname: Ohara
authors.forename: Michiko
authors.initials: M
authors.lastname: Owaki
authors.forename: Kenji
authors.initials: K
authors.lastname: Sonomoto
mesh_terms: D000446:Aldehyde-Lyases / Q000378:metabolism*; D003198:Computer Simulation; D006019:Glycolysis / Q000502:physiology*; D007700:Kinetics; D013294:Lactococcus lactis / Q000378:metabolism*; D008657:Metabolic Clearance Rate; D008954:Models, Biological; D010427:Pentose Phosphate Pathway / Q000502:physiology*; D014994:Xylose / Q000378:metabolism*
chemical_list: D014994:Xylose; D000446:Aldehyde-Lyases; C022367:phosphoketolase