title: [The mechanism of ginsenosides Rg1 and Rb1 promoting glutamic acid release from PC12 cells].
abstract: OBJECTIVE
  To study the mechanism of ginsenosides Rg1 and Rb1 promoting glutamic acid release from PC12 cells.
  
  
  METHODS
  The amount of glutamic acid released from PC12 cells was measured by high performance liquid chromatography (HPLC). The effect of Rg1 and Rb1 on the phosphorylation of synapsins was detected with immunofluorescent staining and Western blotting.
  
  
  RESULTS
  Both Rg1 (10 micromol x L(-1)) and Rb1 (10 micromol x L(-1)) increased glutamic acid release from PC12 cells. The release of glutamic acid was decreased by pre-incubating with the PKA inhibitor H89. H89 inhibited the release of glutamic acid induced by Rb1, but had no effect on the release of glutamic acid induced by Rg1. Moreover, Rb1 enhanced the phosphorylation of synapsins via PKA pathway, Rg1 was out of touch with this.
  
  
  CONCLUSIONS
  Rb1 may promote release of neurotransmitters by increasing the phosphorylation of synapsins via PKA pathway, whereas the up-regulation of neurotransmitters release induced by Rg1 is independent of the phosphorylation of synapsins.
authors.affiliation: Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
authors.forename: Jian-Fei
authors.initials: JF
authors.lastname: Xue
authors.forename: Jin-Feng
authors.initials: JF
authors.lastname: Hu
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Liu
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Jun-Tian
authors.initials: JT
authors.lastname: Zhang
authors.forename: Nai-Hong
authors.initials: NH
authors.lastname: Chen
mesh_terms: D000818:Animals; D015153:Blotting, Western; D017868:Cyclic AMP-Dependent Protein Kinases / Q000502:physiology; D005455:Fluorescent Antibody Technique; D036145:Ginsenosides / Q000494:pharmacology*; D018698:Glutamic Acid / Q000378:metabolism*; D007546:Isoquinolines / Q000494:pharmacology; D018377:Neurotransmitter Agents / Q000378:metabolism*; D016716:PC12 Cells; D010766:Phosphorylation; D051381:Rats; D013449:Sulfonamides / Q000494:pharmacology; D016704:Synapsins / Q000378:metabolism
chemical_list: D036145:Ginsenosides; D007546:Isoquinolines; D018377:Neurotransmitter Agents; D013449:Sulfonamides; D016704:Synapsins; D018698:Glutamic Acid; C442759:ginsenoside Rb1; D017868:Cyclic AMP-Dependent Protein Kinases; C063509:N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide; C035054:ginsenoside Rg1