title: Using running subtraction to detect the wavefront of cortical spreading depression.
abstract: The purpose of this paper was to present a simple method for analyzing the optical images from cortical spreading depression (CSD). The experimental data was acquired by optical intrinsic signal imaging (OISI) in Sprague-Dawley rats. In order to characterize the spatiotemporal evolution of CSD, especially the wavefront, running subtraction and background subtraction were both applied to process the series of images. By comparing the output results of the two different methods, running subtraction was more sensitive to the first transient and small phase of CSD (i.e. wavefront), and the extracted spatial pattern of CSD showed less vascular artifacts. For these advantages, running subtraction proved to be an appropriate approach to detect the wavefront of CSD.
authors.affiliation: Key Laboratory of Biomedical Photonics of Ministry of Education, Huazhong University of Science and Technology, Wuhan 430074, P.R. China (e-mail: hbnu@163.com).
authors.forename: Shangbin
authors.initials: S
authors.lastname: Chen
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Li
authors.forename: Weihua
authors.initials: W
authors.lastname: Luo
authors.forename: Shaoqun
authors.initials: S
authors.lastname: Zeng
authors.forename: Qingming
authors.initials: Q
authors.lastname: Luo