Lordosis facilitation by LHRH, PGE2 or db-cAMP requires activation of the kinase A signaling pathway in estrogen primed rats.
Dose-response curves for lordosis and proceptive behaviors were obtained for luteinizing hormone releasing hormone (LHRH), prostaglandin E2 (PGE2) and dibutyryl cyclic AMP (db-cAMP), by infusing them in the right lateral ventricle (i.c.v.) of ovariectomized (OVX) estradiol benzoate (E2B; 2 microg) treated rats. Two dose levels, one producing the maximal effect and the other one producing a submaximal response (approximately ED50) were selected for testing the capacity of Rp-cAMPS, a kinase A blocker, to modify the behavioral response to the three compounds. I.c.v. injections of Rp-cAMPS, significantly depressed both lordosis and proceptive responses induced by LHRH, PGE2 and db-cAMP. The results show that these agents use the cAMP-kinase A signaling pathway to elicit their stimulating effect on estrous behavior in the rat.
Ramírez-Orduña
Juan Manuel
JM
Centro de Investigación en Reproducción Animal CINVESTAV-Universidad Autónoma de Tlaxcala, Mexico.
Lima-Hernández
Francisco Javier
FJ
García-Juárez
Marcos
M
González-Flores
Oscar
O
Beyer
Carlos
C
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000494:pharmacology; D017868:Cyclic AMP-Dependent Protein Kinases / Q000502:physiology*; D015232:Dinoprostone / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D005300:Fertility Agents, Female / Q000494:pharmacology*; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D007276:Injections, Intraventricular; D010052:Ovariectomy; D010120:Oxytocics / Q000494:pharmacology*; D011187:Posture; D051381:Rats; D012726:Sexual Behavior, Animal / Q000187:drug effects*; D015398:Signal Transduction / Q000187:drug effects; D013873:Thionucleotides / Q000494:pharmacology
D004967:Estrogens; D005300:Fertility Agents, Female; D010120:Oxytocics; D013873:Thionucleotides; D007987:Gonadotropin-Releasing Hormone; C015929:8-((4-chlorophenyl)thio)cyclic-3',5'-AMP; D004958:Estradiol; D003994:Bucladesine; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases; D015232:Dinoprostone