Consumption of a novel dietary formula of plant sterol esters of canola oil fatty acids, in a canola oil matrix containing 1,3-diacylglycerol, reduces oxidative stress in atherosclerotic apolipoprotein E-deficient mice.
The antiatherogenic properties of a novel dietary formula (PS-CO) of plant sterol esters of fatty acids, produced by enzymatic interesterification of plant sterols with canola oil (CO), in a CO matrix containing 1,3-diacylglycerol, were evaluated in apolipoprotein E-deficient mice. PS-CO consumption strongly tended to lower total plasma cholesterol levels by 21%, compared to the placebo group. Blood triglycerides were reduced by 38% and 36% compared to CO and placebo-fed mice, respectively. Serum lipid peroxide levels were lowered following PS-CO administration by 62% and 63%, compared to CO and placebo administration, respectively. Unlike CO supplementation, PS-CO consumption preserved serum paraoxonase (PON1) activity. Mouse peritoneal macrophages from PS-CO-fed mice exhibited reduced cellular uptake of oxidized-LDL compared to those from placebo-fed mice and demonstrated a tendency toward a decreased capability to release superoxide anions. These findings indicate that PS-CO supplementation is beneficial in reducing serum lipid levels, and serum and macrophage oxidative stress, thus contributing to the reduction in atherogenic risk factors.
Fuhrman
Bianca
B
The Lipid Research Laboratory, Technion Faculty of Medicine and Rambam Medical Center, 31096 Haifa, Israel.
Plat
Dorit
D
Herzog
Yael
Y
Aviram
Michael
M
D000818:Animals; D000975:Antioxidants / Q000032:analysis; D001057:Apolipoproteins E / Q000172:deficiency; D050197:Atherosclerosis / Q000097:blood / Q000209:etiology / Q000628:therapy*; D004032:Diet; D004075:Diglycerides / Q000008:administration & dosage*; D004952:Esters / Q000008:administration & dosage; D005227:Fatty Acids / Q000737:chemistry*; D005229:Fatty Acids, Monounsaturated / Q000008:administration & dosage / Q000737:chemistry*; D008055:Lipids / Q000097:blood; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D010840:Phytosterols / Q000008:administration & dosage*; D000074262:Rapeseed Oil
D000975:Antioxidants; D001057:Apolipoproteins E; D004075:Diglycerides; D004952:Esters; D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D008055:Lipids; D010840:Phytosterols; D000074262:Rapeseed Oil