title: Effect of various surface treatments on the microleakage and ultrastructure of resin-tooth interface.
abstract: This study evaluated the effect of collagen removal and sodium ascorbate treatment of acid-etched dentin on the microleakage and ultrastructure of resin-tooth interface under moist and dry conditions using an acetone-based 1 bottle adhesive system. Class V cavities were made on the buccal surfaces of 90 premolars scheduled for orthodontic extraction. The cavities were etched with 37% phosphoric acid (DPI tooth conditioning gel/India) for 15 seconds. The teeth were divided into six groups with 15 teeth each. In Group 1, the etched surface was blot dried with a dry cotton pellet, leaving it visibly moist, and Prime & Bond NT (Dentsply Detrey/ Germany) was applied. In Group 2, after acid conditioning, the cavity surface was air dried for five seconds, followed by application of Prime & Bond NT. In Group 3, 3% NaOCl (Hyposol, Prevest Denpro Ltd/India) was applied to the acid-conditioned cavity surface for two minutes. The surface was blot dried before bonding. In Group 4, after NaOCl treatment, the surface was air dried for five seconds, followed by application of the bonding agent. In Group 5, 10% sodium ascorbate (chemically pure) was applied to the NaOCl-treated acid conditioned tooth surface for one minute. The surface was blot dried before bonding. In Group 6, after sodium ascorbate treatment as in Group 5, the cavity surface was air dried for five seconds before bonding. The cavities were restored with the hybrid composite Spectrum TPH (Dentsply Detrey, Konstanz, Germany). The teeth were extracted immediately after restoration, and the specimens were prepared for microleakage testing using 2% methylene blue dye and for scanning electron microscopic evaluation. The results of the dye penetration were analyzed with Kruskal-Wallis non-parametric analysis followed by the Mann-Whitney U test at a significance level of p = 0.05. After acid etching, the conventional acid etched groups and groups with NaOCl treatment demonstrated extensive leakage. Sodium ascorbate treatment of the NaOCl-treated dentin significantly reduced microleakage. No statistically significant difference between moist and dry bonding was observed in all groups. Although resin tag penetration improved in both the NaOCl-treated and NaOCl/ascorbate-treated groups, an absence of gap at the resin dentin interface was observed only for the NaOCl/ascorbate-treated groups.
authors.affiliation: Department of Operative Dentistry & Endodontics, Government Dental College, Rohtak, Haryana, India.
authors.forename: Rajni
authors.initials: R
authors.lastname: Nagpal
authors.forename: Sanjay
authors.initials: S
authors.lastname: Tewari
authors.forename: Ruchi
authors.initials: R
authors.lastname: Gupta
mesh_terms: D001205:Ascorbic Acid / Q000627:therapeutic use*; D004396:Coloring Agents; D003763:Dental Leakage / Q000175:diagnosis / Q000517:prevention & control*; D003804:Dentin / Q000187:drug effects* / Q000648:ultrastructure; D006801:Humans; D008855:Microscopy, Electron, Scanning; D012973:Sodium Hypochlorite / Q000627:therapeutic use*; D012997:Solvents / Q000627:therapeutic use*; D018709:Statistics, Nonparametric; D013499:Surface Properties
chemical_list: D004396:Coloring Agents; D012997:Solvents; D012973:Sodium Hypochlorite; D001205:Ascorbic Acid