Oligothiophene molecular beacons.
Oligomers of thiophene are widely studied compounds for their electronic and optoelectronic properties. Despite their strong fluorescence, their use as markers for biomolecules, especially for oligonucleotides (ONs), is still largely unexplored. Here, we describe the synthesis of a series of ON molecular beacons employing different oligothiophenes as fluorescent probes and discuss their fluorescence emissions in comparative experiments with and without dabcyl as a quencher, in their hairpin and linear conformations, and as duplexes after hybridization with a complementary target.
Cazzato
Antonella
A
Istituto per la Sintesi Organica e la Fotoreattività del Consiglio Nazionale delle Ricerche, Bologna, Italy.
Capobianco
Massimo L
ML
Zambianchi
Massimo
M
Favaretto
Laura
L
Bettini
Cristian
C
Barbarella
Giovanna
G
D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000737:chemistry* / Q000378:metabolism; D009841:Oligonucleotides / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013876:Thiophenes / Q000737:chemistry* / Q000378:metabolism
D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D009841:Oligonucleotides; D013876:Thiophenes