Protective effect of steamed American ginseng (Panax quinquefolius L.) on V79-4 cells induced by oxidative stress.
Heat-processed Asian ginseng roots (Panax ginseng C.A. Meyer), also known as "red ginseng" in Asia, are reported to have more bioactivity than the no-processed white ginseng roots. Therefore, American fresh ginseng roots (Panax quinquefolius L.) were processed to the red ginseng and examined changes in bioactivity during heating process. The fresh America ginseng roots were steamed at 100 degrees C for 30, 60, 90 and 120 min, and their bioactivities were examined by analyzing the content of ginsenosides and total phenolics, and measuring DPPH and superoxide radical scavenging acivity and their protective effects on V79-4 cells viability and lipid peroxidation. The heating treatment proportionally increased total ginsenosides (4.97%, w/w) content compared with white ginseng (3.27%) and total phenolics from 444.5 mg GAE/100 g to 489.6-574.2 mg GAE/100 g. The antioxidant activity also increased from 285 mg/100 g (vitamin C equivalent) to 353-487 mg/100 g. Heated ginseng showed high levels of DPPH radical scavenging activity (59.5-88.5%) and the high level of superoxide radical scavenging activity (44.2-90.9%). The heated ginseng protected cell viability against H2O2-induced oxidative damage, and enhanced the activities of superoxide dismutase and catalase by dose dependently in V79-4 cells.
Kim
Kyung Tack
KT
Korea Food Research Institute, San 46-1, Baekhyun-Dong, Bundang-Ku, Songnam-Si, Kyunggi-Do 463-746, Republic of Korea.
Yoo
Kyung Mi
KM
Lee
Jin Wook
JW
Eom
Seok Hyun
SH
Hwang
In Kyeong
IK
Lee
Chang Y
CY
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D001713:Biphenyl Compounds; D002374:Catalase / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000494:pharmacology; D005609:Free Radicals / Q000378:metabolism; D036145:Ginsenosides / Q000302:isolation & purification / Q000494:pharmacology; D006358:Hot Temperature; D006861:Hydrogen Peroxide; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D005894:Panax / Q000737:chemistry*; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology; D008517:Phytotherapy; D010851:Picrates; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D018517:Plant Roots; D010946:Plants, Medicinal / Q000737:chemistry; D013482:Superoxide Dismutase / Q000187:drug effects
D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D005609:Free Radicals; D036145:Ginsenosides; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D002374:Catalase; D013482:Superoxide Dismutase