Inflammatory lipoproteins purified from a toxigenic and arthritogenic strain of Mycoplasma arthritidis are dependent on Toll-like receptor 2 and CD14.
Mycoplasma arthritidis is a naturally occurring murine pathogen, and the disease model has been used extensively to understand inflammatory mechanisms. Recently, Triton X-114 extracts of a virulent strain of M. arthritidis were found to be more potent in activating macrophages than were those from an avirulent strain, suggesting a role in disease. Here, octyl glucoside extraction of cells was used to identify four distinct bioactive moieties, with molecular masses of approximately 41, 37, 34, and 17 kDa. Their bioactivities were resistant to proteinase K but were destroyed by alkaline hydrolysis and oxidation. As for MALP-2, all were dependent upon Toll-like receptor 2, but unlike MALP-2, they were also dependent upon CD14. The M. arthritidis lipoproteins exhibited infrared absorbances at 2,900 cm(-1) and 1,662 cm(-1), similar to those seen in Pam(3)-Cys-Ser-(Lys)(4). Edman degradation failed to reveal N-terminal sequences, suggesting that they were blocked and therefore might be triacylated. However, mass spectrometry of fragments revealed that the 41-kDa moiety, which binds to serum apolipoprotein A-1, had similarity with the recently described MlpD lipoprotein of M. arthritidis.
Hasebe
Akira
A
Division of Rheumatology, Department of Internal Medicine, University of Utah School of Medicine, 30 North 1900 East, Salt Lake City, UT 84132, USA.
Mu
Hong-Hua
HH
Washburn
Leigh R
LR
Chan
Fok V
FV
Pennock
Nathan D
ND
Taylor
Michael L
ML
Cole
Barry C
BC
D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D002457:Cell Extracts / Q000276:immunology; D002460:Cell Line; D002478:Cells, Cultured; D005260:Female; D018950:Lipopolysaccharide Receptors / Q000502:physiology*; D008074:Lipoproteins / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification*; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology*; D017737:Macrophages, Peritoneal / Q000276:immunology; D013058:Mass Spectrometry; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D023421:Models, Animal; D008970:Molecular Weight; D045645:Mycoplasma arthritidis / Q000276:immunology* / Q000472:pathogenicity; D013055:Spectrophotometry, Infrared; D051195:Toll-Like Receptor 2 / Q000502:physiology*
D001426:Bacterial Proteins; D002457:Cell Extracts; D018950:Lipopolysaccharide Receptors; D008074:Lipoproteins; D051195:Toll-Like Receptor 2