Experimental model for the study of chronic renal ischemia in rats: morphologic, histological and ultra-structural analysis.
OBJECTIVE
To evaluate a model of chronic renal ischemia in rats and to characterize the effects on renal tissue.


METHODS
168 Wistar rats were divided into two equal groups, control (GC) and ischemia (GI). The animals of the GI (n=84) were submitted to partial ligation of the left renal artery, and the animals of GC (n=84) stayed with the renal artery intact. In seven successive and identical periods of time, in weekly intervals, 12 animals of each group were submitted to nephrectomy, with morphometric determinations and histological and ultra-structural analysis.


RESULTS
The GI presented progressive reduction in renal weight, volume and cortical thickness observed from the 7th day of the experiment, reaching maximum degree in the 49th day (p < 0.05). The proximal tubular atrophy in the GI was observed in 75/84 analysis (89,2%), with highly significant difference among the groups starting from the 7th day (p=0 .0009) and in the other periods of the experiment (p=0 .00001). The most prevalent interstitial alteration was the infiltrate, present in 98,8% of the GI, with highly significant difference among the groups in the whole experiment (p=0 .00001). Ultra-structural analysis didn't demonstrate glomerular lesions, evidencing that the glomerule preserves its intact architecture during chronic ischemia.


CONCLUSIONS
The model showed that chronic renal ischemia in rats provokes progressive renal atrophy, with preservation of glomerular structure.
Konopka
Clóvis Luís
CL
Department of Surgery, Federal University of Santa Maria, 97015-190 Santa Maria, Rio Grande do Sul, Brazil. konopka@terra.com.br
Jurach
Alexandre
A
Wender
Orlando Carlos Belmonte
OC
D000704:Analysis of Variance; D000818:Animals; D001284:Atrophy / Q000473:pathology; D002908:Chronic Disease; D004195:Disease Models, Animal; D005260:Female; D006977:Hypertension, Renal / Q000473:pathology*; D007511:Ischemia / Q000209:etiology / Q000473:pathology*; D007668:Kidney / Q000098:blood supply* / Q000601:surgery; D007678:Kidney Glomerulus / Q000473:pathology; D007687:Kidney Tubules, Proximal / Q000473:pathology; D008026:Ligation; D008854:Microscopy, Electron; D009392:Nephrectomy; D009395:Nephritis, Interstitial / Q000209:etiology / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D012078:Renal Artery Obstruction / Q000150:complications / Q000473:pathology; D018709:Statistics, Nonparametric