A semiempirical approach to the intra-phycocyanin and inter-phycocyanin fluorescence resonance energy-transfer pathways in phycobilisomes.
A semiempirical methodology to model the intra-phycocyanin and inter-phycocyanin fluorescence resonance energy-transfer (FRET) pathways in the rods of the phycobilisomes (PBSs) from Fremyella diplosiphon is presented. Using the Förster formulation of FRET and combining experimental data and PM3 calculation of the dipole moments of the aromatic portions of the chromophores, transfer constants between pairs of chromophores in the phycocyanin (PC) structure were obtained. Protein docking of two PC hexamers was used to predict the optimal distance and axial rotation angle for the staked PCs in the PBSs' rods. Using the distance obtained by the docking process, transfer constants between pairs of chromophores belonging to different PC hexamers were calculated as a function of the angle of rotation. We show that six preferential FRET pathways within the PC hexameric ring and 15 pathways between hexamers exist, with transfer constants consistent with experimental results. Protein docking predicted the quaternary structure for PCs in rods with inter-phycocyanin distance of 55.6 A and rotation angle of 20.5 degrees . The inter-phycocyanin FRET constant between chromophores at positions beta(155) is maximized at the rotation angle predicted by docking revealing the crucial role of this specific inter-phycocyanin channel in defining the complete set of FRET pathways in the system.
Matamala
Adelio R
AR
Facultad de Ciencias Químicas, Universidad de Concepción, Casilla 160-C, Edmundo Larenas 129, Concepción, Chile. amatamal@udec.cl
Almonacid
Daniel E
DE
Figueroa
Maximiliano F
MF
Martínez-Oyanedel
José
J
Bunster
Marta C
MC
D031541:Fluorescence Resonance Energy Transfer; D008958:Models, Molecular; D008968:Molecular Conformation; D045524:Phycobilisomes / Q000737:chemistry*; D010798:Phycocyanin / Q000737:chemistry*
D045524:Phycobilisomes; D010798:Phycocyanin