[Repair alveolar cleft bone defects with bone marrow stromal cells].
OBJECTIVE
To explore the feasibility of repairing alveolar cleft bone defects with bone marrow stromal cells.


METHODS
Total 7 patients of alveolar cleft were included in this study. The hBMSCs were isolated by percoll gradient centrifugation from patient's bone marrow aspirated from iliac crest. The hBMSCs were cultured in vitro and induced to become osteogenic cells in the DMEM medium containing 10% self-serum, beta-glycerophosphate (10 nmol/L) dexamethasone (10(-8) mol/L) , L-2-ascorbic acid(50 micromol/L), and 1, 25 (OH)2 VD3 (10 nmol/L). Induced hBMSCs of passage 3 were harvested and seeded onto partly demineralized allogenic bone matrix (pDBM) to form a cell-scaffold construct and in vitro co-culture for 1 week. The defects were repaired with the cell-scaffold construct. All cases were followed up for 3, 6 months post-operation as short-term evaluation and 1 to 3 years post-operation as long-term evaluation by three-dimensional computerized tomography (3D-CT) and clinical examination.


RESULTS
3D-CT demonstrated that engineered bone was formed in 3 months post-operation. Additionally, formed bone maintained stable up to 1 - 3 years without absorption.


CONCLUSIONS
Engineered bone can be used to repair clinical alveolar cleft bone defects.
Chai
Gang
G
Department of Plastic and Reconstructive Surgery, Ninth People's Hospital, Shanghai Second Medical University, Shanghai Tissue Engineering Center, Shanghai 200011, China.
Zhang
Yan
Y
Hu
Xiao-jie
XJ
Wang
Min
M
Liu
Wei
W
Cui
Lei
L
Cao
Yi-lin
YL
D000293:Adolescent; D000328:Adult; D001854:Bone Marrow Cells; D001861:Bone Regeneration; D018786:Bone Substitutes; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells; D017154:Stromal Cells / Q000166:cytology / Q000637:transplantation; D023822:Tissue Engineering / Q000379:methods*; D020390:Tooth Socket / Q000293:injuries / Q000601:surgery*; D055815:Young Adult
D018786:Bone Substitutes