[Establishment of immortal lymphoblastoid cell bank of keloids pedigree].
OBJECTIVE
To provide perpetual research materials for long term studies by establishing immortal lymphoblastoid cell bank of keloids pedigree.


METHODS
The immortal lymphoblastoid cell lines of keloids pedigree were established by Epstein-Barr virus transformation of peripheral blood B lymphocytes.


RESULTS
27 immortal lymphoblastoid cell lines of keloids pedigree were obtained successfully, all of the immortal lymphoblastoid cell lines were successfully revivificated after been frozen in liquid nitrogen.


CONCLUSIONS
It is important to establish immortal lymphoblastoid cell bank of keloids pedigree and provide long-term DNA materials for deep study of keloids in the future.
Song
Mei
M
Department of Plastic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Gao
Jian-hua
JH
Yan
Xin
X
Liu
Xiao-jun
XJ
Chen
Yang
Y
D000293:Adolescent; D000328:Adult; D000368:Aged; D001402:B-Lymphocytes; D018929:Cell Culture Techniques; D002461:Cell Line, Transformed; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007627:Keloid / Q000097:blood / Q000235:genetics*; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D014015:Tissue Banks; D055815:Young Adult