Ubiquitous and uniform in vivo fluorescence in ROSA26-EGFP BAC transgenic mice.
Transplantation studies and cell lineage analyses require the ability to explicitly distinguish morphologically identical cells that have an identifiable marker indicating their origin in vivo. Several reporter mouse strains have been generated for such studies, but pancellular detection of the marker in all tissues has not been achieved. In this report, we describe the generation of transgenic mice that express enhanced green fluorescent protein (EGFP) under control of a 187 kb bacterial artificial chromosome (BAC) containing the murine ROSA26 locus, and show several advantages over existing EGFP reporter lines. It is demonstrated that EGFP is ubiquitously and reproducibly expressed from the murine BAC transgene in all organs and tissues analyzed, including the hematolymphoid compartment. Using this new reporter strain in hematopoietic cell transplantation studies, it is demonstrated that leukocytes in recipients maintain uniform transgene expression and are easily distinguished by flow cytometric analysis of live cells. The results suggest that the ROSA26 BAC is an efficient strategy for expressing complex transgene cassettes in vivo.
Giel-Moloney
Maryann
M
Division of Gastroenterology, GRASP Digestive Disease Center, Tufts-New England Medical Center, Boston, Massachusetts, USA.
Krause
Daniela S
DS
Chen
Gang
G
Van Etten
Richard A
RA
Leiter
Andrew B
AB
D000818:Animals; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic; D011506:Proteins / Q000235:genetics*; D022661:RNA, Untranslated
C110853:Gt(ROSA)26Sor non-coding RNA, mouse; D011506:Proteins; D022661:RNA, Untranslated; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins