title: The role of the surface chemistry of CoCr alloy particles in the phagocytosis and DNA damage of fibroblast cells.
abstract: Surface chemistry of CoCr particles is demonstrated to be fundamental to the process of phagocytosis by fibroblast cells in vitro. Particles preincubated in serum for 5 days and washed in water before addition to cell cultures were phagocytosed less readily than were particles preincubated in minimal essential medium (MEM) for 1 h and washed in water. This was explained by the coating of calcium phosphate and protein on the serum-immersed particles investigated by time-of-flight secondary ion mass spectroscopy. The cells incubated with the serum-immersed particles had a reduced mitotic index when compared with the MEM-immersed particles, indicating that the phagocytosed particles were causing cell cycle arrest. The release of soluble ions measured by electrothermal atomic absorption spectroscopy within the first hour of particle immersion in MEM was identified as the most likely cause for the DNA damage measured by single cell gel electrophoresis ("Comet" assay). Cryofocused ion beam SEM with a spatial resolution of 8 nm was used to cross section cells, to investigate the location of the phagocytosed particles, some of which were found within the nuclear membrane. This paper demonstrated that consideration of the surface chemistry is essential to understand the processes of the effects of orthopedic wear debris.
authors.affiliation: Interface Analysis Centre, University of Bristol, 121 St. Michael's Hill, Bristol BS2 8BS, United Kingdom.
authors.forename: A C
authors.initials: AC
authors.lastname: Lewis
authors.forename: D
authors.initials: D
authors.lastname: Ladon
authors.forename: P J
authors.initials: PJ
authors.lastname: Heard
authors.forename: L
authors.initials: L
authors.lastname: Peto
authors.forename: I
authors.initials: I
authors.lastname: Learmonth
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000633:toxicity*; D002453:Cell Cycle / Q000187:drug effects; D002478:Cells, Cultured; D002858:Chromium Alloys / Q000737:chemistry* / Q000633:toxicity*; D004249:DNA Damage; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010587:Phagocytosis; D011475:Prosthesis Failure; D018629:Spectrometry, Mass, Secondary Ion; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D002858:Chromium Alloys