Protein engineering.
This chapter focuses on protein engineering strategies that aim to increase the chances of obtaining crystals suitable for X-ray diffraction. The chapter is divided into three main parts: one dealing with protein engineering through a bioinformatics approach, the second focusing on DNA modifications via random mutagenesis, and the third describing a nonexhaustive number of in vitro modifications based on site-directed mutagenesis.
Longhi
Sonia
S
Architecture et Fonction des Macromolécules Biologiques, Centre National de la Recherche Scientifique, Universités Aix-Marseille I et II, Marseille, France.
Ferron
François
F
Egloff
Marie-Pierre
MP
D001483:Base Sequence; D003460:Crystallization; D004247:DNA / Q000235:genetics / Q000378:metabolism; D016296:Mutagenesis; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D015202:Protein Engineering / Q000379:methods*
D004247:DNA