Identification of an inflammation-inducible serum protein recognized by anti-disialic acid antibodies as carbonic anhydrase II.
Acute-phase proteins are an important marker of inflammation and sometimes have a role in the general defense response towards tissue injury. In the present study, we identified a 32-kDa protein that was immunoreactive with monoclonal antibody 2-4B (mAb.2-4B), which is specific to di/oligoNeu5Gc structures, and that behaved as an acute-phase protein following stimulation with either turpentine oil or lipopolysaccharides. The 32-kDa protein was identified as carbonic anhydrase II (CA-II), based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analyses of the purified protein. Mouse and human CA-II was immunoreactive and immunoprecipitated with mAb.2-4B, but contained no sialic acid. In addition to mAb.2-4B, the mAb. S2-566 an antibody specific for diNeu5Ac-containing glycans, recognized the CA-II, whereas an anti-oligo/polysialic acid antibody did not. These results indicate that a part of the CA-II protein structure mimics the disialic acid structure recognized by the monoclonal antibodies. This is the first report that CA-II circulates in the serum following inflammation.
Yasukawa
Zenta
Z
Laboratory of Animal Cell Function, Bioscience and Biotechnology Center, Nagoya University, Nagoya 464-8601, Japan.
Sato
Chihiro
C
Kitajima
Ken
K
D000209:Acute-Phase Proteins / Q000032:analysis*; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D024402:Carbonic Anhydrase II / Q000097:blood* / Q000276:immunology; D002417:Cattle; D002846:Chromatography, Affinity; D006801:Humans; D007249:Inflammation / Q000097:blood* / Q000139:chemically induced; D008070:Lipopolysaccharides; D051379:Mice; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D012794:Sialic Acids / Q000276:immunology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014425:Turpentine
D000209:Acute-Phase Proteins; D000911:Antibodies, Monoclonal; D008070:Lipopolysaccharides; D012794:Sialic Acids; D024402:Carbonic Anhydrase II; D014425:Turpentine