title: Hydrogen peroxide ototoxicity in unblocking ventilation tubes: a chinchilla pilot study.
abstract: OBJECTIVE
  Some clinicians use hydrogen peroxide (H2O2) to clear the lumen of ventilation tubes that become blocked postoperatively. The ototoxicity associated with H2O2 has been controversial.
  
  
  METHODS
  We designed an experiment to test if H2O2 damages the cochlear hair cells using a Chinchilla laniger animal model.
  
  
  METHODS
  Nine chinchillas (18 ears) were included in this study. Each animal was used as its own control. Following the insertion of ventilation tubes in both ears and baseline recordings of the auditory brain stem responses (ABR), we instilled 2 ml of 3 percent H2O2 into their right external auditory canals (experimental ears). H2O2 was left in the external auditory canal for a total of 5 minutes and then was drained. We instilled a normal saline control solution in their left ears (control ears) in a similar fashion. ABR recordings were performed 1 day after the last instillation of H2O2 and 5 days later.
  
  
  RESULTS
  There was no statistically significant difference in the ABR thresholds of the experimental and control ears.
  
  
  CONCLUSIONS
  H2O2 did not appear to cause ototoxicity in chinchilla ears with tympanostomy tubes exposed to H2O2 instillation using a standard clinical protocol.
authors.affiliation: Department of Otolaryngology, McGill University Health Care Centre/Montreal Children's Hospital, Quebec, Canada.
authors.forename: Marc-Elie
authors.initials: ME
authors.lastname: Nader
authors.forename: Maria
authors.initials: M
authors.lastname: Kourelis
authors.forename: Sam Joseph
authors.initials: SJ
authors.lastname: Daniel
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage / Q000633:toxicity*; D002682:Chinchilla; D016057:Evoked Potentials, Auditory, Brain Stem / Q000187:drug effects; D005260:Female; D006198:Hair Cells, Auditory / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000008:administration & dosage / Q000633:toxicity*; D007322:Instillation, Drug; D008876:Middle Ear Ventilation / Q000009:adverse effects*; D010865:Pilot Projects
chemical_list: D000891:Anti-Infective Agents, Local; D006861:Hydrogen Peroxide