title: Structural and functional analysis of hypothetical proteins in mouse hippocampus from two-dimensional gel electrophoresis.
abstract: Protein profiling in five individual mouse strains showed strain-specific expression of three hypothetical proteins (HPs). As functional and structural assignment of HPs were based on predictions and low identity to known structures, HPs were identified by MALDI-TOF/TOF, and their proposed tentative function was determined by enzyme assays. Three identified HPs were extracted from gels and renatured, and pyridoxal phosphate phosphatase, inorganic pyrophosphate phosphatase, and antioxidant activities were revealed, findings in agreement with functional predictions.
authors.affiliation: Department of Pediatrics, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria.
authors.forename: Leila
authors.initials: L
authors.lastname: Afjehi-Sadat
authors.forename: Jae-Won
authors.initials: JW
authors.lastname: Yang
authors.forename: Arnold
authors.initials: A
authors.lastname: Pollak
authors.forename: Dae-Won
authors.initials: DW
authors.lastname: Kim
authors.forename: Soo-Young
authors.initials: SY
authors.lastname: Choi
authors.forename: Gert
authors.initials: G
authors.lastname: Lubec
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D006624:Hippocampus / Q000737:chemistry*; D013058:Mass Spectrometry; D051379:Mice; D008958:Models, Molecular; D008962:Models, Theoretical; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000032:analysis*; D010446:Peptide Fragments / Q000737:chemistry; D011487:Protein Conformation; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D009419:Nerve Tissue Proteins; D010446:Peptide Fragments