NRAMP1 and TNF-alpha polymorphisms and susceptibility to tuberculosis in Thais.
OBJECTIVE
Polymorphisms in the natural resistance-associated macrophage protein gene 1 (NRAMP1) and tumour necrosis factor (TNF)-alpha gene have been found to be associated with susceptibility to tuberculosis in different populations. However, the results are inconsistent. This study aimed to determine whether NRAMP1 and TNF-alpha variants are associated with tuberculosis in Thais.


METHODS
Polymorphisms of NRAMP1 at INT4, D543N and the 3' untranslated region, and of TNF-alpha at +488, -238, and -308, were examined in 149 tuberculosis patients and 147 healthy controls. PCR using sequence-specific oligonucleotides and sequence-specific priming were used to genotype the NRAMP1 and TNF polymorphisms, respectively.


RESULTS
There were no significant differences in the distribution of the genotype frequencies for the NRAMP1 and TNF-alpha polymorphisms between the patients and controls.


CONCLUSIONS
The NRAMP1 and TNF-alpha genes are not associated with susceptibility to tuberculosis in Thais.
Vejbaesya
Sasijit
S
Department of Transfusion Medicine, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand. sisve@mahidol.ac.th
Chierakul
Nitipatana
N
Luangtrakool
Panpimon
P
Sermduangprateep
Chutima
C
D000293:Adolescent; D000328:Adult; D000368:Aged; D027682:Cation Transport Proteins / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D015995:Prevalence; D013785:Thailand / Q000453:epidemiology; D014376:Tuberculosis / Q000453:epidemiology / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D000099173:Natural Resistance-associated Macrophage Protein 1
D027682:Cation Transport Proteins; D004247:DNA; D014409:Tumor Necrosis Factor-alpha; D000099173:Natural Resistance-associated Macrophage Protein 1