title: Discrimination of Cryptosporidium species by denaturing gradient gel electrophoresis.
abstract: Denaturing gradient gel electrophoresis (DGGE) was used for the discrimination of three species and one genotype of the protozoan parasite Cryptosporidium: the C. parvum, C. andersoni, C. muris, and C. muris Japanese field mouse genotype. A set of primers specific for the 18S rRNA gene of Cryptosporidium was used in the DGGE; consequently, the four strains showed different banding patterns. This is a potentially convenient and precise method for the discrimination of Cryptosporidium spp.
authors.affiliation: Laboratory of Animal Health and Management, Graduate School of Agricultural Science, Tohoku University, Naruko-Onsen, Osaki City, Miyagi, 989-6711, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Satoh
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Nakai
mesh_terms: D000818:Animals; D001483:Base Sequence; D003458:Cryptosporidium / Q000145:classification* / Q000235:genetics*; D016172:DNA Fingerprinting; D016054:DNA, Protozoan / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005838:Genotype; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation; D012337:RNA, Ribosomal, 18S / Q000235:genetics*
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S