title: Intestinal intraluminal injection of glutamine increases trolox total equivalent antioxidant capacity (TEAC) in hepatic ischemia-reperfusion.
abstract: OBJECTIVE
  To evaluate the effects of intraluminal injection of glutamine on the serum trolox equivalent antioxidant capacity in an experimental model of ischemia-reperfusion of the liver observing the applicability of modifications on the original assay method.
  
  
  METHODS
  Thirty Wistar rats underwent laparotomy to perform a 20 cm blind sac of small bowel and occlusion of the hepatic hilo for 30 minutes and reperfusion for 5 minutes. Into the gut sac it was injected glutamine (glutamine group, n=10) or distilled water (control group, n=10). Ten other animals (sham group) underwent laparotomy without artery occlusion. Blood samples were collected for trolox equivalent antioxidant capacity assays in different temperature conditions, reagent quantities and time for spectrophotometer readings.
  
  
  RESULTS
  Total antioxidant capacity was significantly greater in glutamine group than in both control group (1.60[1.55-1.77] vs 1.44[1.27-1.53]) and sham group (1.60[1.55-1.77] vs 1.48[1.45-1.59]).
  
  
  CONCLUSIONS
  Glutamine enhanced serum antioxidant capacity. The assay technique consistently reflected the changes in the antioxidant defenses in this experimental model.
authors.affiliation: Department of Surgery, Federal University of Mato Grosso, Brazil.
authors.forename: Alberto Bicudo
authors.initials: AB
authors.lastname: Salomão
authors.forename: José Eduardo
authors.initials: JE
authors.lastname: Aguilar-Nascimento
authors.forename: Sandro
authors.initials: S
authors.lastname: Percário
authors.forename: Victor
authors.initials: V
authors.lastname: Sano
authors.forename: Nicole Ribeiro
authors.initials: NR
authors.lastname: Marques
authors.forename: Claudia Cristina Gomes de Oliveira
authors.initials: CC
authors.lastname: Dias
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism*; D002839:Chromans / Q000097:blood* / Q000737:chemistry; D004195:Disease Models, Animal; D005973:Glutamine / Q000494:pharmacology*; D007421:Intestine, Small / Q000187:drug effects*; D008099:Liver / Q000098:blood supply*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy*
chemical_list: D000975:Antioxidants; D002839:Chromans; D005973:Glutamine; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid