title: A quantitative proteomic analysis of light adaptation in a globally significant marine cyanobacterium Prochlorococcus marinus MED4.
abstract: In this study, we conducted biological and technical replicate proteomic experiments using isobaric tags for relative and absolute quantification (iTRAQ), to elucidate the light adaptation strategies of Prochlorococcus marinus MED4. The MED4 strain is adapted to an oceanic environment characterized by low nutrient levels, and ever-changing light intensities. Approximately 11% of the proteome was identified, with an average coefficient of variation of iTRAQ quantification values of 0.15. Fifteen proteins were deemed to be statistically and significantly differentially expressed in changing light intensities, particularly the down-regulation of photosystem-related proteins, and the up-regulation of the stress-related chaperone GroEL in high light compared to low light.
authors.affiliation: Biological and Environmental Systems Group, Department of Chemical and Process Engineering, The University of Sheffield, Mappin Street, Sheffield S1 3JD, United Kingdom.
authors.forename: Jagroop
authors.initials: J
authors.lastname: Pandhal
authors.forename: Phillip C
authors.initials: PC
authors.lastname: Wright
authors.forename: Catherine A
authors.initials: CA
authors.lastname: Biggs
mesh_terms: D000221:Adaptation, Ocular; D001426:Bacterial Proteins / Q000032:analysis*; D000458:Cyanobacteria / Q000737:chemistry / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial / Q000528:radiation effects; D015335:Molecular Probes; D009792:Oceans and Seas; D048929:Prochlorococcus / Q000737:chemistry* / Q000502:physiology*; D040901:Proteomics / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D015335:Molecular Probes