Gene structure and transcription of IRF-1 and IRF-7 in the mandarin fish Siniperca chuatsi.
The genes of IRF-1 and IRF-7 have been cloned from the mandarin fish (Siniperca chuatsi). The IRF-1 gene has 4919 nucleotides (nt) and contains 10 exons and 9 introns, with an open reading frame (ORF) of 903nt encoding 301aa. The IRF-7 gene has 6057nt and also contains 10 exons and 9 introns, with an ORF of 1308nt encoding 436aa. The IRF-1 and IRF-7 genes have only one copy each in the genome. The transcription of IRF-1 and IRF-7 in different organs was analyzed by real-time PCR, and both molecules were constitutively expressed. The IRF-1 and IRF-7 mRNAs were abundant in gill, spleen, kidney and pronephros. The temporal transcriptional changes for IRF-1, IRF-7 and Mx were investigated within 48h after poly I: C stimulation in liver, gill, spleen and pronephros. An increased transcription was detected for IRF-1 and IRF-7 12h post-stimulation, being earlier than the transcription of Mx protein; however, IRF-1 and IRF-7 transcription decreased while the Mx protein was stable at 48h post-stimulation.
Sun
B J
BJ
State Key Laboratory of Freshwater Ecology and Biotechnology, and Laboratory of Fish Diseases, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province 430072, PR China.
Chang
M X
MX
Song
Y
Y
Yao
W J
WJ
Nie
P
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern / Q000662:veterinary; D003001:Cloning, Molecular; D050856:Interferon Regulatory Factor-1 / Q000096:biosynthesis / Q000235:genetics*; D050839:Interferon Regulatory Factor-7 / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D010473:Perciformes / Q000235:genetics* / Q000276:immunology; D010802:Phylogeny; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D014158:Transcription, Genetic
D050856:Interferon Regulatory Factor-1; D050839:Interferon Regulatory Factor-7; D012313:RNA