title: Histopathologic changes of acellularized xenogenic carotid vascular grafts implanted in a pig-to-goat model.
abstract: BACKGROUND
  The present study was designed to determine the in vivo patency and recellularization pattern of acellularized small-diameter xenogenic arterial grafts. We implanted acellularized porcine carotid arteries in bilateral carotid arteries of goats and microscopically analyzed the recellularization pattern of these grafts with the recipient's cells over time.
  
  
  METHODS
  Carotid arteries of pigs weighing 30-40 kg were harvested and decellularized with hypertonic saline followed by sodium dodecyl sulfate. Acellularized porcine carotid vascular xenografts (0.4-0.5 cm in diameter) were prepared into 4 cm-long segments and implanted bilaterally in the carotid arteries of 10 black-haired goats. The in vivo patency of the implanted acellularized xenogenic grafts was evaluated at regular intervals by color Doppler ultrasonography. The goats were sacrificed at predetermined intervals (1 week, 1 month, 3 months, 6 months, 12 months after implantation), two animals at each interval. Upon retrieval, visual inspections and histopathologic examinations of the grafts were performed. To identify smooth muscle cells and functioning endothelial cells, immunohistochemical staining for alpha-smooth muscle actin and von Willebrand factor were also performed.
  
  
  CONCLUSIONS
  All experimental animals survived the observation period. Nineteen out of 20 implanted grafts showed patency with no thrombi. Microscopic analysis revealed that the grafts were completely covered with the hosts' endothelial cells, beginning from anastomotic sites. The grafts were gradually recellularized with recipients'cells including fibroblasts, myofibroblasts and smooth muscle cells. In conclusion, this study suggested that acellularized xenogenic vascular grafts can be a good alternative for the small-diameter vascular graft.
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Samsung Seoul Hospital Sungkyunkwan University School of Medicine, Seoul, Korea.
authors.forename: W S
authors.initials: WS
authors.lastname: Kim
authors.forename: J W
authors.initials: JW
authors.lastname: Seo
authors.forename: J R
authors.initials: JR
authors.lastname: Rho
authors.forename: W G
authors.initials: WG
authors.lastname: Kim
mesh_terms: D000818:Animals; D001705:Bioprosthesis; D002339:Carotid Arteries / Q000166:cytology / Q000637:transplantation*; D049109:Cell Proliferation; D004730:Endothelium, Vascular / Q000166:cytology; D006041:Goats; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D034421:Sus scrofa; D023822:Tissue Engineering; D018615:Ultrasonography, Doppler, Color; D014654:Vascular Patency