Endothelin-1 and its receptors ET(A) and ET(B) in drug-induced gingival overgrowth.
BACKGROUND
The purpose of this study was to study the expression of endothelin-1 (ET-1) and its receptors ETA and ETB in normal human gingiva and cyclosporin-induced gingival fibroblasts.


METHODS
Gingival samples were collected from eight normal healthy individuals, eight patients with periodontitis, and eight patients with cyclosporin A (CsA)-induced gingival overgrowth. Total RNA was extracted from tissue samples, and reverse transcriptase-polymerase chain reaction was performed for ET-1, ETA, and ETB. ET-1 protein was estimated from the tissues by enzyme-linked immunosorbent assay. The expression of ET-1 and its receptors was also examined in gingival fibroblast cells treated with CsA.


RESULTS
ET-1 mRNA expression was significantly higher in patients with CsA-induced gingival overgrowth (P <0.001) than in patients with periodontitis and the controls. ETA mRNA was expressed more than the ETB in all examined samples. In human gingival fibroblasts, ET-1 expression was increased with CsA incorporation compared to controls (P <0.001).


CONCLUSIONS
These results suggest that CsA can modulate the expression of ET-1 in gingival fibroblasts and CsA-induced gingival overgrowth.
Tamilselvan
S
S
Department of Periodontics, Sri Ramachandra Dental College and Hospital, Sri Ramachandra Medical College and Research Institute, Deemed University, Porur, Chennai, Tamilnadu, India.
Raju
Surya Narayana
SN
Loganathan
D
D
Kamatchiammal
S
S
Abraham
Georgie
G
Suresh
R
R
D000328:Adult; D016022:Case-Control Studies; D002478:Cells, Cultured; D016572:Cyclosporine / Q000633:toxicity*; D019332:Endothelin-1 / Q000096:biosynthesis*; D005260:Female; D005347:Fibroblasts / Q000187:drug effects; D019214:Gingival Overgrowth / Q000139:chemically induced* / Q000378:metabolism*; D006801:Humans; D007166:Immunosuppressive Agents / Q000633:toxicity*; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000378:metabolism; D044022:Receptor, Endothelin A / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D019332:Endothelin-1; D007166:Immunosuppressive Agents; D044022:Receptor, Endothelin A; D016572:Cyclosporine