title: Purification, partial characterization, crystallization and preliminary X-ray diffraction of a novel cardiotoxin-like basic protein from Naja naja atra (South Anhui) venom.
abstract: A novel cardiotoxin-like basic protein was isolated from the venom of the Chinese cobra (Naja naja atra) from the south of Anhui in China. The protein inhibits the expression of vascular endothelial growth factor and basic fibroblast growth factor in human lung cancer cell line H1299 and induces the haemolysis of rabbit erythrocytes under low-lecithin conditions. After a two-step chromatographic purification, the resultant 7 kDa protein was crystallized by the hanging-drop vapour-diffusion method at room temperature. A complete data set was collected to 2.35 A resolution using an in-house X-ray diffraction system. The crystal belongs to space group P4(1)2(1)2, with unit-cell parameters a = b = 43.2, c = 147.9 A. There are two molecules in the crystallographic asymmetric unit.
authors.affiliation: Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230027, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Rong
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Xiao-hua
authors.initials: XH
authors.lastname: Lou
authors.forename: Xio
authors.initials: X
authors.lastname: Zhang
authors.forename: Yong-xiang
authors.initials: YX
authors.lastname: Gao
authors.forename: Mai-kun
authors.initials: MK
authors.lastname: Teng
authors.forename: Li-wen
authors.initials: LW
authors.lastname: Niu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004179:Cobra Cardiotoxin Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D003460:Crystallization; D018360:Crystallography, X-Ray; D004546:Elapid Venoms / Q000737:chemistry*; D017815:Elapidae; D004912:Erythrocytes / Q000187:drug effects; D005260:Female; D006461:Hemolysis / Q000187:drug effects; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D011817:Rabbits; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D004179:Cobra Cardiotoxin Proteins; D004546:Elapid Venoms