Rhythmic cycle of clathrin-coated pit formation at the trans-golgi network in human MDA-MB-435 cells.
We studied the in vivo dynamics of enhanced green fluorescent protein-tagged clathrin light chain a (GFP-CLCa) at the trans-Golgi network (TGN) in MDA-MB-435 cells. The intensity of fluorescence signals of GFP-CLCa periodically increased and decreased at the TGN approximately every 100 s. This suggests that the formation of clathrin-coated pits occurs synchronously and periodically at the TGN.
Sakaushi
Shinji
S
Laboratory of Molecular Biology and Cell Informatics, Division of Bioscience and Informatics, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, Japan. ssaka@biochem.osakafu-u.ac.jp
Senda-Murata
Kaori
K
Fukada
Takashi
T
Oka
Shigenori
S
Sugimoto
Kenji
K
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002966:Clathrin / Q000096:biosynthesis*; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D051379:Mice; D008856:Microscopy, Fluorescence; D018715:Microscopy, Video; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D021601:trans-Golgi Network / Q000378:metabolism* / Q000648:ultrastructure
D002966:Clathrin; D049452:Green Fluorescent Proteins