title: Alteration of monoamine concentrations in the brain of medaka, Oryzias latipes, exposed to tributyltin.
abstract: We measured the concentrations of monoamines in the brain of Japanese medaka, Oryzias latipes, exposed to tributyltin (TBT). Fish were exposed to 0, 1, 5, 25, or 125 microg g(-1) of TBT via the diet for 21 days. After the administration period, six males and six females in each treatment group were dissected and their brains were collected. The following monoamines were analyzed: dopamine (DA), norepinephrine (NE), epinephrine (E), and 5-hydroxytryptamine (5-HT). The metabolites of DA, 3,4-dihydroxyphenylacetic acid and homovanilic acid, and the metabolite of 5-HT, 5-hydroxyindolacetic acid were also analyzed. The concentration of DA in the brain of male medaka and the concentrations of 5-HT and NE in the female brains were significantly decreased by exposure to 125 microg TBT g(-1). The concentrations of 5-HT and NE in males and of DA in females were slightly decreased by 125 micrg g(-1) of TBT, although the differences were not statistically significant. The present study demonstrates that TBT alters monoamine concentrations in the brain of medaka.
authors.affiliation: Center for Marine Environmental Studies, Ehime University, 2-5 Bunkyo-cho, Matsuyama 790-8577, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Nakayama
authors.forename: Yuji
authors.initials: Y
authors.lastname: Oshima
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Tachibana
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Furuse
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Honjo
mesh_terms: D015102:3,4-Dihydroxyphenylacetic Acid / Q000032:analysis / Q000378:metabolism; D000818:Animals; D015306:Biogenic Monoamines / Q000032:analysis / Q000378:metabolism*; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D004298:Dopamine / Q000032:analysis / Q000378:metabolism; D004837:Epinephrine / Q000032:analysis / Q000378:metabolism; D005260:Female; D006719:Homovanillic Acid / Q000032:analysis / Q000378:metabolism; D007211:Indoles / Q000032:analysis / Q000378:metabolism; D008297:Male; D009638:Norepinephrine / Q000032:analysis / Q000378:metabolism; D009990:Oryzias; D012701:Serotonin / Q000032:analysis / Q000378:metabolism; D013997:Time Factors; D014220:Trialkyltin Compounds / Q000633:toxicity*; D014873:Water Pollutants / Q000633:toxicity*
chemical_list: D015306:Biogenic Monoamines; D007211:Indoles; D014220:Trialkyltin Compounds; D014873:Water Pollutants; D015102:3,4-Dihydroxyphenylacetic Acid; D012701:Serotonin; C011559:tributyltin; C018079:5-(hydroxy-3-indolyl)lactic acid; D004298:Dopamine; D009638:Norepinephrine; D006719:Homovanillic Acid; D004837:Epinephrine