title: Photodynamic inactivation of in vitro bacterial cultures from pressure ulcers.
abstract: OBJECTIVE
  To evaluate in vitro the antibacterial effect of diode laser light of wavelength 650 nm, in association with the photosensitive substance toluidine blue, on the bacteria in infected skin ulcers.
  
  
  METHODS
  Samples were collected by means of swabs containing a medium for transporting infected material from skin ulcers. The material was inoculated into culturing medium containing azide blood agar for the growth of Gram-positive bacteria, and MacConkey agar for Gram-negative bacteria, and incubated for 48 hours. The results obtained from counting the colony-forming units were correlated and subjected to statistical analysis, adopting the significance level of p > or = 0.05.
  
  
  RESULTS
  From analysis of variance (ANOVA), the result for the general mean was p = 0.0215. Using the t test with post-hoc test, the result for TBO vs. Control was p = 0.0186, and for TBO + Laser vs. Control it was p = 0.0039.
  
  
  CONCLUSIONS
  There was a significant reduction in colony-forming units when the cultures were subjected to photodynamic therapy.
authors.affiliation: Department of Physiotherapy, UNIDERP, Brazil. ptpaulo@terra.com.br
authors.forename: Paulo de Tarso Camillo de
authors.initials: Pde T
authors.lastname: Carvalho
authors.forename: Ana Paula da Costa
authors.initials: AP
authors.lastname: Marques
authors.forename: Felipe Abdalla dos
authors.initials: FA
authors.lastname: Reis
authors.forename: Ana Carulina Guimarães
authors.initials: AC
authors.lastname: Belchior
authors.forename: Iandara Schettert
authors.initials: IS
authors.lastname: Silva
authors.forename: Carlos Alexandre
authors.initials: CA
authors.lastname: Habitante
authors.forename: Daniela Aparecida
authors.initials: DA
authors.lastname: Sussai
mesh_terms: D000704:Analysis of Variance; D015169:Colony Count, Microbial; D006090:Gram-Negative Bacteria / Q000528:radiation effects*; D006094:Gram-Positive Bacteria / Q000528:radiation effects*; D006801:Humans; D054023:Lasers, Semiconductor / Q000627:therapeutic use*; D028022:Low-Level Light Therapy; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology* / Q000627:therapeutic use; D003668:Pressure Ulcer / Q000188:drug therapy / Q000382:microbiology / Q000532:radiotherapy*; D014048:Tolonium Chloride / Q000494:pharmacology
chemical_list: D017319:Photosensitizing Agents; D014048:Tolonium Chloride