Simultaneous detection of Pseudomonas fragi, P. lundensis, and P. putida from meat by use of a multiplex PCR assay targeting the carA gene.
Species-specific primers and a multiplex PCR assay were developed for the simultaneous identification and differentiation of Pseudomonas fragi, P. lundensis, and P. putida based on the coamplification of different portions of the small subunit of the carbamoyl phosphate synthase gene (carA). The carA multiplex PCR was used to detect the presence of the three Pseudomonas species from beef, chicken, and pork samples and proved to be effective in showing their evolution during the storage of meat.
Ercolini
Danilo
D
Dipartimento di Scienza degli Alimenti, Università degli Studi di Napoli Federico II, via Università 100, 80055 Portici, NA, Italy. ercolini@unina.it
Russo
Federica
F
Blaiotta
Giuseppe
G
Pepe
Olimpia
O
Mauriello
Gianluigi
G
Villani
Francesco
F
D000818:Animals; D001483:Base Sequence; D002222:Carbamoyl-Phosphate Synthase (Ammonia) / Q000235:genetics*; D017931:DNA Primers; D004463:Ecology; D008460:Meat / Q000382:microbiology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D011549:Pseudomonas / Q000235:genetics / Q000302:isolation & purification*; D044206:Pseudomonas fragi / Q000235:genetics / Q000302:isolation & purification*; D016958:Pseudomonas putida / Q000235:genetics / Q000302:isolation & purification*
D017931:DNA Primers; D002222:Carbamoyl-Phosphate Synthase (Ammonia)