[Multi-dimensional fingerprints of Japan Jiuxin pill].
OBJECTIVE
To develop HPLC-UV/ELSD and HPLC-UV/MS3 fingerprints of Japan Jiuxin pill using multi-dimensional hyphenated methods and study on its chemical base.


METHODS
Separation was performed on Altima C18 analytical column (250 mm x 4.6 mm ID, 5 microm). The mobile phases consisted of water containing 0.2% formic acid and acetonitrile was used as gradient elute. The flow rate was 1.0 mL x min(-1). An electrospray ion trap mass spectrometer was utilized for qualitative analysis and both positive and negative secondary ion scan mode were applied.


RESULTS
HPLC-UV/ELSD and HPLC-UV/ MS3 fingerprints of Japan Jiuxin pill with well separation and reproducibility were obtained. Totally 21 components in the complex formula were identified and the chemical structural information of glycocholic acid and its isomer were suggested.


CONCLUSIONS
The research is propitious to offer an effective and reliable pattern for analyzing Japanese Kampo Medicines as well as its quality control.
Zhang
Min
M
Jiangxi College of Traditional Chinese Medicine, Nanchang 330006, China.
Wang
Yi-Ming
YM
Luo
Guo-An
GA
D002317:Cardiovascular Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D006331:Heart Diseases / Q000188:drug therapy*; D006801:Humans; D007564:Japan; D008516:Medicine, Chinese Traditional; D021241:Spectrometry, Mass, Electrospray Ionization
D002317:Cardiovascular Agents