Selection of appropriate organic additives for enhancing Zn and Cd phytoextraction by hyperaccumulators.
Chelant-enhanced phytoextraction is one of the most promising technologies to remove heavy metals from soil. The key of the technology is to choose suitable additives in combination with a suitable plant. In the present study, laboratory batch experiment of metal solubilization, cress seeds germination were undertaken to investigate the metal-mobilizing capability and the phytotoxicity of organic additives, including ethylene diamine triacetic acid (EDTA), citric acid, acetic acid, oxalic acid, glutamine and monosodium glutamate waste liquid (MGWL) from food industry. Experiments in pots were carried out to study the effects of the additives on Zn and Cd phytoextraction. Furthermore, a leaching experiment with lysimeter was performed to evaluate the environmental risks of additive-induced leaching to underground water. The results showed that EDTA had a strong mobilizing ability for Zn and Cd, followed by mixed reagent (MR) and MGWL. MGWL and acetic acid at 5 mmol equivalent per liter resulted in seed germination index less than 2%. Experiments in pots verified the phytotoxicity of acetic acid and MGWL. Addition of the mixed reagent at 6-10 mmol/kg significantly increased Zn phytoextraction by Thlaspi caerulescens. The same for EDTA and the mixed reagent at 10 mmol/kg by Sedum alfredii. But only mixed reagents could significantly increase Cd phytoextraction by the studied hyperaccumulators. This suggested that the strong chelant was not always the good agent to enhance phytoextraction. S. alfredii combined with 2-10 mmol/kg soil MR was preferred for phytoremediation of Cd/Zn contaminated soils in southern China, this could result in high phytoextraction of Cd/Zn and reduce the leaching risk to underground water than EDTA assisted phytoextration.
Wu
Qi-tang
QT
College of Natural Resource and Environment, South China Agricultural University, Guangzhou 510642, China. qitangwu@pub.guangzhou.gd.cn
Deng
Jin-chuan
JC
Long
Xin-xian
XX
Morel
Jean-louis
JL
Schwartz
Christophe
C
D019342:Acetic Acid; D001673:Biodegradation, Environmental; D002104:Cadmium / Q000378:metabolism* / Q000493:pharmacokinetics; D002614:Chelating Agents / Q000378:metabolism*; D002681:China; D019343:Citric Acid; D004492:Edetic Acid; D004787:Environmental Pollution / Q000517:prevention & control*; D019649:Food Industry; D018525:Germination / Q000187:drug effects; D005973:Glutamine; D019815:Oxalic Acid; D031963:Sedum / Q000187:drug effects / Q000378:metabolism*; D012970:Sodium Glutamate; D012989:Soil Pollutants / Q000378:metabolism* / Q000493:pharmacokinetics; D031227:Thlaspi / Q000187:drug effects / Q000378:metabolism*; D014866:Waste Products / Q000032:analysis; D015032:Zinc / Q000378:metabolism* / Q000493:pharmacokinetics
D002614:Chelating Agents; D012989:Soil Pollutants; D014866:Waste Products; D002104:Cadmium; D005973:Glutamine; D019343:Citric Acid; D019815:Oxalic Acid; D004492:Edetic Acid; D015032:Zinc; D019342:Acetic Acid; D012970:Sodium Glutamate