title: Ginsenoside Rb1 protects against damage to the spiral ganglion cells after cochlear ischemia.
abstract: The effects of transient cochlear ischemia on spiral ganglion cells (SGCs) were studied in Mongolian gerbils. Ischemic insult was induced by occluding the bilateral vertebral arteries of gerbils for 15min. Seven days after ischemia, the percentage of SGCs decreased to 67.5% from the preischemic baseline in the basal turn. Evaluation with immunohistochemical staining showed TUNEL-positive reactions in the SGCs with fragmented nuclei. In addition, we investigated the protective effects of ginsenoside Rb1 (gRb1) against ischemic injury to SGCs. Seven days after ischemia, the auditory brainstem response threshold shift was significantly reduced and the percentage of SGCs decreased to 90.2% from the preischemic baseline in the basal turn in the gRb1-treated group. These findings suggest that gRb1 prevented hearing loss caused by ischemic injury to SGCs in Mongolian gerbils.
authors.affiliation: Department of Otolaryngology, Ehime University School of Medicine, Ehime, Japan.
authors.forename: Kensuke
authors.initials: K
authors.lastname: Fujita
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Hakuba
authors.forename: Ryuji
authors.initials: R
authors.lastname: Hata
authors.forename: Isao
authors.initials: I
authors.lastname: Morizane
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yoshida
authors.forename: Masachika
authors.initials: M
authors.lastname: Shudou
authors.forename: Masahiro
authors.initials: M
authors.lastname: Sakanaka
authors.forename: Kiyofumi
authors.initials: K
authors.lastname: Gyo
mesh_terms: D000161:Acoustic Stimulation; D000818:Animals; D002452:Cell Count / Q000379:methods; D015834:Cochlear Diseases / Q000188:drug therapy / Q000473:pathology* / Q000503:physiopathology; D016057:Evoked Potentials, Auditory, Brain Stem / Q000187:drug effects / Q000502:physiology; D005849:Gerbillinae; D036145:Ginsenosides / Q000494:pharmacology* / Q000627:therapeutic use; D020287:In Situ Nick-End Labeling / Q000379:methods; D007511:Ischemia / Q000188:drug therapy / Q000473:pathology* / Q000503:physiopathology; D046529:Microscopy, Electron, Transmission / Q000379:methods; D009474:Neurons / Q000187:drug effects* / Q000648:ultrastructure; D018696:Neuroprotective Agents / Q000494:pharmacology* / Q000627:therapeutic use; D013136:Spiral Ganglion / Q000473:pathology*; D051020:bcl-X Protein / Q000378:metabolism
chemical_list: D036145:Ginsenosides; D018696:Neuroprotective Agents; D051020:bcl-X Protein; C442759:ginsenoside Rb1