Modulation of transforming growth factor beta2 (TGF-beta2) by inositol hexaphosphate in colon carcinogenesis in rats.
OBJECTIVE
To evaluate modulation in the expression of Transforming growth factor beta2 (TGF-beta2) in short-term colon carcinogenesis.


METHODS
64 male rats was used, comprising 4 groups of 16 animals each: group 1 received Inositol hexaphosphate (IP6) and azoxymethane (AOM); group 2, AOM alone; group 3, IP6 alone; group 4 was used as control. Groups 1 and 3 were given 1% IP6 in drinking water for 6 weeks. AOM was administered subcutaneously at weeks 3 and 4 of the experiment at 20 mg/kg of body weight each week. Immunohistochemical processing was performed with the use of anti-TGF-beta2 primary antibodies in right colon samples and quantitation of TGF-beta2 as percentage of expression, through computer-assisted image processing.


RESULTS
mean values of TGF-beta2 expression were 9.0 +/- 3.9% for group 4 (control), 12.7 +/- 4.0% for group 3 (IP6), 19.3 +/- 6.2% for group 2 (AOM), and 13.1 +/- 5.3% for group 1 (IP6+AOM). The value of p was calculated as 0.0001 for a 5% or lower significance level.


CONCLUSIONS
the experiment revealed a significant increase in TGF-beta2 expression in right colon with the administration of AOM, and a significant decrease in TGF-beta2 expression when IP6 was administered with AOM.
Marks
Guido
G
Department of Surgery, UFMS, Brazil. gmarks@brturbo.com.br
Aydos
Ricardo Dutra
RD
Fagundes
Djalma José
DJ
Pontes
Elenir Rose Jardim Cury
ER
Takita
Luiz Carlos
LC
Amaral
Eva Glória Abrão Siufi do
EG
Rossini
Airton
A
Ynouye
Celso Massaschi
CM
D000818:Animals; D001397:Azoxymethane; D015197:Carcinogenicity Tests; D002273:Carcinogens; D003106:Colon / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D004195:Disease Models, Animal; D007091:Image Processing, Computer-Assisted; D008297:Male; D010833:Phytic Acid / Q000627:therapeutic use*; D051381:Rats; D053781:Transforming Growth Factor beta2 / Q000187:drug effects* / Q000378:metabolism
D002273:Carcinogens; D053781:Transforming Growth Factor beta2; D010833:Phytic Acid; D001397:Azoxymethane