Molecular characterisation of canine nonsteroidal anti-inflammatory drug-activated gene (NAG-1).
Nonsteroidal anti-inflammatory drug (NSAID)-activated gene (NAG-1), a divergent member of the transforming growth factor beta superfamily, was previously identified as a gene induced by several anti-tumorigenic compounds, including NSAIDs and peroxisome proliferator-activated receptor gamma (PPARgamma) ligands in humans. In this study, canine NAG-1 was characterised from a canine genomic database. Gene induction by some NSAIDs and PPARgamma ligands was demonstrated in canine osteosarcoma cell lines. Phylogenetic analysis indicates that canine NAG-1 is more homologous with the corresponding mouse and rat genes than with human NAG-1. Expression of canine NAG-1 was increased by treatment with piroxicam and SC-560 (NSAIDs) and the PPARgamma ligand rosiglitazone. This study demonstrates that canine NAG-1 is up-regulated by some anti-tumorigenic compounds in osteosarcoma cell lines and may provide an important target of chemotherapy in canine cancer.
Yamaguchi
Kiyoshi
K
Department of Pathobiology, College of Veterinary Medicine, University of Tennessee, Knoxville, TN 37996, USA.
Whitlock
Nichelle C
NC
Liggett
Jason L
JL
Legendre
Alfred M
AM
Fry
Michael M
MM
Baek
Seung Joon
SJ
D000595:Amino Acid Sequence; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology / Q000627:therapeutic use; D045744:Cell Line, Tumor; D016207:Cytokines / Q000235:genetics*; D004283:Dog Diseases / Q000188:drug therapy; D004285:Dogs; D005786:Gene Expression Regulation / Q000187:drug effects; D055436:Growth Differentiation Factor 15; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D012516:Osteosarcoma / Q000188:drug therapy / Q000662:veterinary; D047495:PPAR gamma / Q000494:pharmacology / Q000627:therapeutic use; D010802:Phylogeny; D012313:RNA / Q000032:analysis; D051381:Rats; D016415:Sequence Alignment; D013045:Species Specificity; D015533:Transcriptional Activation; D015854:Up-Regulation
D000894:Anti-Inflammatory Agents, Non-Steroidal; D016207:Cytokines; C526140:GDF15 protein, human; C526141:Gdf15 protein, mouse; C526142:Gdf15 protein, rat; D055436:Growth Differentiation Factor 15; D047495:PPAR gamma; D012313:RNA