Efficient gene silencing induction in tomato by a viral satellite DNA vector.
Virus-induced gene silencing (VIGS) in tomato (Lycopersicon esculentum Mill.) is currently routinely analysed using Tobacco rattle virus (TRV)-based vector. We recently reported a new vector system modified from DNA beta (DNAm beta) of Tomato yellow leaf curl China virus (TYLCCNV) for VIGS analysis in Solanaceous species including tomato. Here, we describe DNAm beta-induced gene silencing in tomato. We found that DNAm beta-induced gene silencing was initiated from vascular tissues, and later scattered to other tissues. Once initiated in seedlings, the silencing phenotype lasted for the entire life span of the plants, was expressed in a variety of tissues and organs including leaf, shoot, stem, flower and fruit, and could be achieved at any growth stage. It was insensitive to temperature as high as 32 degrees C and no symptoms were observed in silenced plants. The DNAm beta vector worked efficiently in at least seven tomato cultivars, indicating that this system has great potential as a versatile VIGS system for routine functional analysis of genes in tomato.
Cai
Xinzhong
X
State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, PR China.
Wang
Changchun
C
Xu
Youping
Y
Xu
Qiufang
Q
Zheng
Zhong
Z
Zhou
Xueping
X
D004276:DNA, Satellite / Q000235:genetics*; D017791:Geminiviridae / Q000235:genetics; D020868:Gene Silencing; D005822:Genetic Vectors; D018551:Solanum lycopersicum / Q000235:genetics* / Q000254:growth & development; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013696:Temperature
D004276:DNA, Satellite