title: Effects of budesonide and probiotics enemas on the colonic mucosa of rats with experimental colitis.
abstract: OBJECTIVE
  To investigate the effect of enemas containing probiotics and budesonide on the colonic mucosa in experimental colitis.
  
  
  METHODS
  Fifty male Wistar rats with experimental colitis induced by 10% acetic acid enema were randomized to five groups (10 rats each) according to the treatment: group 1--saline solution, group 2--budesonide (0.75 mg/kg/day), group 3--probiotics (1mg/day), group 4--probiotics plus budesonide, and group 5--control, with not-treated rats. The following variables were studied: body weight, macroscopic and microscopic score of the colonic mucosa, and DNA content of the mucosa.
  
  
  RESULTS
  All animals lost weight between the beginning and the end of the experiment (280+ 16 mg versus 249+21 mg, p< 0.001). There was no significant difference among the groups in relation to both the macroscopic and histological score. The budesonide + probiotic group showed higher DNA content than control group (1.24+ 0.15 versus 0.92+ 0.30 mg/100 mg of tissue, p=0.01).
  
  
  CONCLUSIONS
  Budesonide in addition to probiotics enhances the mucosal trophism in experimental colitis.
authors.affiliation: Department of Surgery, Medical School, Federal University of Mato Grosso, Brazil.
authors.forename: Mardem Machado de
authors.initials: MM
authors.lastname: Souza
authors.forename: José Eduardo de
authors.initials: JE
authors.lastname: Aguilar-Nascimento
authors.forename: Maria Helena
authors.initials: MH
authors.lastname: Gomes-da-Silva
authors.forename: Rubens
authors.initials: R
authors.lastname: Carlos Junior
mesh_terms: D019342:Acetic Acid; D000704:Analysis of Variance; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D001706:Biopsy; D001835:Body Weight / Q000187:drug effects; D019819:Budesonide / Q000008:administration & dosage / Q000627:therapeutic use*; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000188:drug therapy* / Q000235:genetics; D004247:DNA / Q000032:analysis; D004195:Disease Models, Animal; D004733:Enema; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000473:pathology; D008297:Male; D019936:Probiotics / Q000008:administration & dosage / Q000627:therapeutic use*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D018709:Statistics, Nonparametric
chemical_list: D000893:Anti-Inflammatory Agents; D019819:Budesonide; D004247:DNA; D019342:Acetic Acid