Exploiting nucleotidylyltransferases to prepare sugar nucleotides.
[reaction: see text] Enzymatic approaches to prepare sugar nucleotides are gaining in importance and offer several advantages over chemical synthesis including high yields and stereospecificity. We report the cloning, expression, and purification of two new wild-type thymidylyltransferases and observed catalysis with a wide variety of substrates. Significant product inhibition was not observed with the enzymes studied over a 24 h period, enabling the efficient preparation of 15 sugar nucleotides, clearly demonstrating the synthetic utility of these biocatalysts.
Timmons
Shannon C
SC
Department of Chemistry, Dalhousie University, Halifax, N.S., Canada B3H 4J3.
Mosher
Roy H
RH
Knowles
Sheryl A
SA
Jakeman
David L
DL
D002241:Carbohydrates / Q000737:chemistry*; D002384:Catalysis; D015394:Molecular Structure; D009711:Nucleotides / Q000737:chemistry* / Q000378:metabolism*; D009713:Nucleotidyltransferases / Q000378:metabolism*; D010710:Phosphates / Q000737:chemistry; D013291:Streptococcus / Q000201:enzymology; D013379:Substrate Specificity
D002241:Carbohydrates; D009711:Nucleotides; D010710:Phosphates; D009713:Nucleotidyltransferases