Interleukin-10 regulated gene expression in cells of hypothalamic-pituitary-adrenal axis origin.
OBJECTIVE
The hypothalamic-pituitary-adrenal (HPA) axis is a mediator for interactions between the immune and neuroendocrine systems. Pro-inflammatory cytokines such as interleukin-1 (IL-1), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) have been shown to activate the HPA axis. Recently, interleukin-10, an important anti-inflammatory cytokine in the immune system, has been shown to be expressed in the central nervous system and neuroendocrine system. Little is known, however, about IL-10's functions in the HPA axis.


METHODS
The Affymetrix DNA microarray (mouse genome U74Av2 Probe Array) was conducted to determine the gene expression profile regulated by IL-10 in cells of HPA axis origin.


RESULTS
In this study, we analyzed gene expression regulated by IL-10 in cells derived from the HPA axis. The results showed that quorums of genes are modulated by IL-10 in these neuroendocrine cells.


CONCLUSIONS
These findings will provide a valuable repository to aid in understanding IL-10's functions in the HPA axis at the molecular level.
Tu
Huolin
H
Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555-1019, USA.
Rady
Peter L
PL
Juelich
Terry
T
Tyring
Stephen K
SK
Koldzic-Zivanovic
Nina
N
Smith
Eric M
EM
Hughes
Thomas K
TK
D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology; D023281:Genomics; D007030:Hypothalamo-Hypophyseal System / Q000166:cytology / Q000276:immunology*; D016753:Interleukin-10 / Q000494:pharmacology* / Q000502:physiology*; D051379:Mice; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D010913:Pituitary-Adrenal System / Q000166:cytology / Q000276:immunology*
D016753:Interleukin-10