Hybrid bioelectrocatalyst for hydrogen peroxide reduction: immobilization of enzyme within organic-inorganic film of structured Prussian Blue and PEDOT.
Fabrication of structured film (on glassy carbon substrate) composed of compact Prussian Blue (that has been prepared by alternate immersions and through assembling within ultra-thin layers of 4(pyrrole-1-yl)-benzoic acid, PPyBA) and poly(3,4-ethylendioxythiophene), PEDOT, is described. This functionalized film has been characterized by fast charge propagation, and it has served as a redox conducting template for permanent attachment of a model enzyme, horseradish peroxidase, HRP. The resulting organic-inorganic system acts as an effective hybrid bioelectrocatalyst for electroreduction of hydrogen peroxide, a model reactant for biosensors and biofuel cells. Among important issues are rigidity, permanence of enzyme attachment, morphology, hydrophilicity, and attractive mediating capabilities of the PEDOT-stabilized Prussian Blue based structured film.
Ernst
Andrzej
A
Department of Chemistry, University of Warsaw, Pasteura 1, PL-02-093 Warsaw, Poland.
Makowski
Oktawian
O
Kowalewska
Barbara
B
Miecznikowski
Krzysztof
K
Kulesza
Pawel J
PJ
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry*; D002244:Carbon / Q000737:chemistry; D002384:Catalysis; D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005295:Ferrocyanides / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000737:chemistry*; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D019086:Bridged Bicyclo Compounds, Heterocyclic; D004800:Enzymes, Immobilized; D005295:Ferrocyanides; D011108:Polymers; C121383:poly(3,4-ethylene dioxythiophene); D002244:Carbon; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; C000170:ferric ferrocyanide