title: Formation of high-resistance supported lipid bilayer on the surface of a silicon substrate with microelectrodes.
abstract: We have developed two basic technologies for fabrication of supported planar lipid bilayer membrane ion channel biosensors: a defect-free lipid bilayer formation on the substrate surface with electrode pores and a patterning technique for the hydrophobic self-assembled-monolayer to form the guard ring that reduces the lipid bilayer edge-leak current. The importance of the supported-membrane structure to achieve low noise and high-speed performance is suggested on the basis of the observed relation between the single-ion-channel current noise and the pore size.
authors.affiliation: Department of Vacuum UV Photoscience, Institute for Molecular Science, The Graduate University for Advanced Studies, Myodaiji, Okazaki, Japan. urisu@ims.ac.jp
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Urisu
authors.forename: Md Mashiur
authors.initials: MM
authors.lastname: Rahman
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Uno
authors.forename: Ryugo
authors.initials: R
authors.lastname: Tero
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Nonogaki
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D003460:Crystallization / Q000379:methods; D017097:Electric Impedance; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D006096:Gramicidin / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D008839:Microelectrodes; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D010316:Particle Size; D013499:Surface Properties
chemical_list: D008051:Lipid Bilayers; D006096:Gramicidin