title: Usefulness of argyrophilic nucleolar organizer regions in detection of lung cells alterations after benzo[a]pyrene instillation.
abstract: OBJECTIVE
  To verify the relationship between AgNOR expression and lung tissues changes of Wistar rats after pulmonary instillation of benzo[a]pyrene (B[a]P).
  
  
  METHODS
  Male Rattus norvegicus albinus,Wistar lineage were given a single intrapulmonary instillation of B[a]P at doses of 10 and 20 mg/kg in a volume of approximately 0.3 ml. After 7 and 21 days the rats were killed and the lung slices submitted to a histological technique of AgNOR. AgNOR dots were quantified and the result analyzed by statistical tests; p < or = 0.05 was considered significant.
  
  
  RESULTS
  The mean values of AgNOR dots for the experimental groups 10/7 (1.51+/-0.86) and 10/21 (1.84+/-0.13) were statistically different (p = 0.009). Among the groups 20/7 (1.63+/-0.11) and 20/21 (2.48+/-0.28) was observed statistically significant difference (p = 0.003).
  
  
  CONCLUSIONS
  The AgNOR technique can be useful in identification of cells changes induced by B[a]P.
authors.affiliation: Postgraduate Program in Health Sciences in the Central West Region UnB and UFMS, Brazil.
authors.forename: Baldomero Antonio Kato da
authors.initials: BA
authors.lastname: Silva
authors.forename: Iandara Schettert
authors.initials: IS
authors.lastname: Silva
authors.forename: Daniel Martins
authors.initials: DM
authors.lastname: Pereira
authors.forename: Ricardo Dutra
authors.initials: RD
authors.lastname: Aydos
authors.forename: Paulo de Tarso Camillo de
authors.initials: Pde T
authors.lastname: Carvalho
mesh_terms: D000818:Animals; D034961:Antigens, Nuclear / Q000378:metabolism; D001564:Benzo(a)pyrene; D014408:Biomarkers, Tumor; D002273:Carcinogens; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D049109:Cell Proliferation / Q000187:drug effects; D008175:Lung Neoplasms / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D008297:Male; D009687:Nuclear Proteins / Q000378:metabolism; D009697:Nucleolus Organizer Region / Q000473:pathology*; D011379:Prognosis; D051381:Rats; D017208:Rats, Wistar; D016622:Silver Staining
chemical_list: D034961:Antigens, Nuclear; D014408:Biomarkers, Tumor; D002273:Carcinogens; D009687:Nuclear Proteins; C051963:nucleolar organizer region associated proteins; D001564:Benzo(a)pyrene