[Expression of ngR mRNA and ngR protein in brain tissues of newborn rats with hypoxic ischemic brain damage].
OBJECTIVE To study the expression of NgR mRNA and NgR protein in the brain tissues of newborn rats with hypoxic ischemic brain damage (HIBD) its implications.


METHODS
Seventy two newborn SD rats (7 days) were randomly divided into two groups, with or without surgical induced HIBD. RT-PCR was used to detect the expression of NgR mRNA. The immunohistochemistry method and the Western Blot were used to detect the distribution and expression of NgR protein in the brain tissues.


RESULTS
Compared with the rats without HIBD, the expression of NgR mRNA and protein in the rats with HIBD increased significantly (P< 0. 05), although the expression started to decrease after 24 hours in HIBD condition.


CONCLUSIONS
Increased NgR mRNA and NgR protein may be associated with the hypoxic-ischemic injury and may inhibit the neuron regeneration. The decreased expression of NgR mRNA and protein at a later stage may be associated with the loss of nerve cells.
Wang
Hua
H
Department of Neonatal, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Yao
Yu-jia
YJ
Chen
Da-peng
DP
D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism* / Q000473:pathology*; D002545:Brain Ischemia / Q000150:complications*; D005260:Female; D058851:GPI-Linked Proteins; D005786:Gene Expression Regulation; D002534:Hypoxia, Brain / Q000150:complications / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D007150:Immunohistochemistry; D008297:Male; D009185:Myelin Proteins / Q000235:genetics* / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D000070816:Nogo Receptor 1; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D058851:GPI-Linked Proteins; D009185:Myelin Proteins; D000070816:Nogo Receptor 1; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; C486581:Rtn4r protein, rat