[Correlation of chemokines and chemokine receptors with clinical features in newly diagnosed systemic lupus erythematosus].
OBJECTIVE
To explore the correlation of chemokines and chemokine receptors with clinical features of newly diagnosed systemic lupus erythematosus (SLE).


METHODS
Samples of venous blood were collected from 37 newly diagnosed SLE patients, 2 males and 35 females, aged 32.5 (15-54), and 20 healthy controls. The serum concentration of macrophage inflammatory protein-1alpha (MIP-1alpha), MIP-1beta and reduced upon activation normal T cell expressed and secreted (RANTES) were measured by ELISA. Samples of anticoagulative serum were collected from 18 of the 37 newly diagnosed SLE patients and 10 healthy controls. The expression rates of CCR1, CCR3, and CCR5 on CD4+ T cells were detected by flow cytometry. Indirect immunofluorescence method was used to detect the anti-ds-DNA antibody, and blot immunoassay was used to detect the anti-RNP and anti-SSA antibodies. The associations of chemokines and chemokine receptors with the clinical features were analyzed.


RESULTS
The serum concentration of MIP-1alpha of the SLE patients was (37 +/- 25) ng/L, significantly higher than that of the controls (8 +/- 9) ng/L (P < 0.001). The serum concentration of MIP-1beta of the SLE patients was (160 +/- 140) ng/L, significantly higher than that of the controls (76 +/- 41) ng/L (P = 0.003). The serum concentration of RANTES of the SLE patients was (184 +/- 22) ng/L, not significantly different from that of the controls (144 +/- 79) ng/L (P > 0.05). The serum concentration of MIP-1alpha of the SLE patients with fever was 52 ng/L +/- 27 ng/L, significantly higher than that of the SLE patients without fever (28 ng/L +/- 19 ng/L, P = 0.006). The serum concentration of MIP-1beta of the SLE patients with arthritis was 221 ng/L +/- 158 ng/L, significantly higher than that of the SLE patients without arthritis (95 ng/L +/- 83 ng/L, P = 0.008). The serum concentration of RANTES of the SLE patients with low blood platelet count was 130 ng/L +/- 122 ng/L, significantly lower than that of the SLE patients with normal blood platelet count (212 ng/L +/- 114 ng/L, P = 0.049). The percentage of CD4+ CCR3+ T cell subgroup in CD4+ T cell in peripheral blood of the SLE patients positive in anti-RNP antibody was 14.8% +/- 3.0%, significantly lower than that of the SLE patients negative in anti-RNP antibody (11.3% +/- 2.6%, P = 0.018).


CONCLUSIONS
Abnormality of different chemokines and chemokine receptors may concern with different clinical features of SLE.
Tao
Jin-hui
JH
Department of Rheumatology and Immunology, Anhui Provincial Hospital, Hefei 23001, China.
Li
Xiang-pei
XP
Li
Xiao-mei
XM
Kan
Ming
M
Zhai
Zhi-min
ZM
Wang
Wei
W
D000293:Adolescent; D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D054405:Chemokine CCL3 / Q000097:blood; D054407:Chemokine CCL4 / Q000097:blood; D018946:Chemokine CCL5 / Q000097:blood; D018925:Chemokines / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D054389:Receptors, CCR1 / Q000097:blood; D054397:Receptors, CCR3 / Q000097:blood; D019713:Receptors, CCR5 / Q000097:blood; D019707:Receptors, Chemokine / Q000096:biosynthesis*
D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018946:Chemokine CCL5; D018925:Chemokines; D054389:Receptors, CCR1; D054397:Receptors, CCR3; D019713:Receptors, CCR5; D019707:Receptors, Chemokine