[Specific cytotoxicity of CTL which was induced by DC pulsed with mannosylated antigen].
OBJECTIVE
To investigate the role of mannosylated tumor antigen in the course of anti-tumor response.


METHODS
L2 domain of HER-2/neu (ErbB2) ectodomain was expressed in E.coli, purified and mannosylated. Dendritic cells(DCs) were induced from human peripheral blood mononuclear cell by GM-CSF and IL-4. The maturation and functional capacity of DCs pulsed with mannosylated L2 (mL2) protein was investigated.


RESULTS
L2 protein could induce DC maturation, which was accompanied by elevated expression of MHC and costimulatory molecules. The effect of mL2 protein on DC maturation was more remarkable than that of non-mannosylated L2 protein. Furthermore, DCs pulsed with mL2 could stimulate high tumor cell lysis by CTL.


CONCLUSIONS
Our experiment provide the foundation for the study of new tumor vaccine by mannosylation.
Zhong
Guo-cheng
GC
Department of Oncology, 452 Hospital, Chengdu 610021, China. zgc005@yahoo.com.cn
Xu
Ming
M
Shi
Ming
M
Hu
Mei-ru
MR
Min
Min
M
Chen
Jian
J
D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000166:cytology; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects*
D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D015847:Interleukin-4; D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human