Production of aminoglycosides in non-aminoglycoside producing Streptomyces lividans TK24.
The pRBM4 cosmid, which harbors a putative cluster of genes spanning a 31.8-kb chromosomal region of the ribostamycin producer Streptomyces ribosidificus ATCC 21294, was heterologously expressed in Streptomyces lividans TK24. ESI-MS/MS, HPLC, and LC-ESI MS analyses showed that the transformation gave rise to ribostamycin production in various culture broths. This is the first report of heterologous aminoglycoside production.
Subba
Bimala
B
Institute of Biomolecule Reconstruction (IBR), Department of Pharmaceutical Engineering, Sun Moon University, #100, Kalsan-ri, Tangjeong-myeon, Asansi, Chungnam 336-708, Republic of Korea.
Kurumbang
Nagendra Prasad
NP
Jung
Young Soo
YS
Yoon
Yeo Joon
YJ
Lee
Hei Chan
HC
Liou
Kwangkyoung
K
Sohng
Jae Kyung
JK
D000617:Aminoglycosides / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D001671:Biochemistry / Q000379:methods; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003360:Cosmids; D015195:Drug Design; D008956:Models, Chemical; D008968:Molecular Conformation; D010957:Plasmids / Q000378:metabolism; D012271:Ribostamycin / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D048170:Streptomyces lividans / Q000378:metabolism*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D012271:Ribostamycin