title: Chromosome 11 aneusomy in esophageal cancers and precancerous lesions--an early event in neoplastic transformation: an interphase fluorescence in situ hybridization study from south India.
abstract: OBJECTIVE
  To detect aneusomic changes with respect to chromosome 11 copy number in esophageal precancers and cancers wherein the generation of cancer-specific phenotypes is believed to be associated with specific chromosomal aneuploidies.
  
  
  METHODS
  We performed fluorescence in situ hybridization (FISH) on esophageal tissue paraffin sections to analyze changes in chromosome 11 copy number using apotome-generated images by optical sectioning microscopy. Sections were prepared from esophageal tumor tissue, tissues showing preneoplastic changes and histologically normal tissues (control) obtained from patients referred to the clinic for endoscopic evaluation.
  
  
  RESULTS
  Our results demonstrated that aneusomy was seen in all the cancers and preneoplastic tissues, while none of the controls showed aneusomic cells. There was no increase in aneusomy from precancers to cancers.
  
  
  CONCLUSIONS
  Our results suggest that evaluation of chromosome 11 aneusomy in esophageal tissue using FISH with an appropriate signal capture-analysis system, can be used as an ancillary molecular marker predictive of early neoplastic changes. Future studies can be directed towards the genes on chromosome 11, which may play a role in the neoplastic transformation of esophageal precancerous lesions to cancers.
authors.affiliation: Department of Genetics & Molecular Medicine, Kamineni Hospitals, LB Nagar, Hyderabad 500068, Andhra Pradesh, India. greenpastures@gmail.com
authors.forename: Vasavi
authors.initials: V
authors.lastname: Mohan
authors.forename: Shivani
authors.initials: S
authors.lastname: Ponnala
authors.forename: Hemakumar M
authors.initials: HM
authors.lastname: Reddy
authors.forename: Radha
authors.initials: R
authors.lastname: Sistla
authors.forename: Rachel A
authors.initials: RA
authors.lastname: Jesudasan
authors.forename: Yog Raj
authors.initials: YR
authors.lastname: Ahuja
authors.forename: Qurratulain
authors.initials: Q
authors.lastname: Hasan
mesh_terms: D000782:Aneuploidy; D002471:Cell Transformation, Neoplastic; D002869:Chromosome Aberrations; D002880:Chromosomes, Human, Pair 11; D004938:Esophageal Neoplasms / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D011230:Precancerous Conditions / Q000235:genetics*