Coronin 3 involvement in F-actin-dependent processes at the cell cortex.
The actin interaction of coronin 3 has been mainly documented by in vitro experiments. Here, we discuss coronin 3 properties in the light of new structural information and focus on assays that reflect in vivo roles of coronin 3 and its impact on F-actin-associated functions. Using GFP-tagged coronin 3 fusion proteins and RNAi silencing we show that coronin 3 has roles in wound healing, protrusion formation, cell proliferation, cytokinesis, endocytosis, axonal growth, and secretion. During formation of cell protrusions actin accumulation precedes the focal enrichment of coronin 3 suggesting a role for coronin 3 in events that follow the initial F-actin assembly. Moreover, we show that coronin 3 similar to other coronins interacts with the Arp2/3-complex and cofilin indicating that this family in general is involved in regulating Arp2/3-mediated events.
Rosentreter
André
A
Center for Biochemistry, Institute of Biochemistry I, Medical Faculty, University of Cologne, Joseph-Stelzmann-Str. 52, 50931 Cologne, Germany.
Hofmann
Andreas
A
Xavier
Charles-Peter
CP
Stumpf
Maria
M
Noegel
Angelika A
AA
Clemen
Christoph S
CS
D051339:Actin Depolymerizing Factors / Q000378:metabolism; D051376:Actin-Related Protein 2-3 Complex / Q000378:metabolism; D000199:Actins / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D002460:Cell Line; D002465:Cell Movement; D049109:Cell Proliferation; D002942:Circular Dichroism; D018360:Crystallography, X-Ray; D003599:Cytoskeleton / Q000378:metabolism; D015536:Down-Regulation; D005347:Fibroblasts / Q000502:physiology; D006801:Humans; D047468:Immunoprecipitation; D008840:Microfilament Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009638:Norepinephrine / Q000378:metabolism; D016716:PC12 Cells; D010873:Pinocytosis; D017434:Protein Structure, Tertiary; D034622:RNA Interference; D051381:Rats
D051339:Actin Depolymerizing Factors; D051376:Actin-Related Protein 2-3 Complex; D000199:Actins; D008840:Microfilament Proteins; C072061:coronin proteins; D009638:Norepinephrine