Analysis of melatonin using a pH- and temperature-responsive aqueous chromatography system.
A new method for the qualitative and quantitative analysis of an intracerebral hormone, such as melatonin, has been proposed, utilizing newly designed copolymers that include ion-exchange groups. These copolymers responded to both the temperature and the pH, and the copolymers were modified with cross-linked hydrogel applied onto aminopropyl silica beads. The products were evaluated as HPLC packing materials for a pH- and temperature-responsive chromatography. The property of the surface of the stationary phase was altered from hydrophilic to hydrophobic, and from charged to non-charged by changes in both the temperature and the pH. In the chromatographic system, we investigated how to change the retention of melatonin by varying the temperature. A pH- and temperature-responsive chromatography is expected to be useful for the separation of pharmaceuticals and biomolecules.
Ayano
Eri
E
Department of Physical Pharmaceutical Chemistry, Kyoritsu University of Pharmacy, Tokyo, Japan.
Suzuki
Yusuke
Y
Kanezawa
Masahito
M
Sakamoto
Chikako
C
Morita-Murase
Yuko
Y
Nagata
Yoshiko
Y
Kanazawa
Hideko
H
Kikuchi
Akihiko
A
Okano
Teruo
T
D000180:Acrylic Resins; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007475:Ion Exchange Resins; D008550:Melatonin / Q000032:analysis*; D012701:Serotonin / Q000031:analogs & derivatives / Q000302:isolation & purification; D013696:Temperature; D014364:Tryptophan / Q000302:isolation & purification
D000180:Acrylic Resins; D007475:Ion Exchange Resins; C052970:poly-N-isopropylacrylamide; D012701:Serotonin; D014364:Tryptophan; D008550:Melatonin; C006389:N-acetylserotonin