Cholesterol depletion inhibits fatty acid uptake without affecting CD36 or caveolin-1 distribution in adipocytes.
Caveolin-1 and CD36 are plasma membrane fatty acid binding proteins that participate in adipocyte fatty acid uptake and metabolism. Both are associated with cholesterol-enriched caveolae/lipid rafts in the plasma membrane that are important for long chain fatty acid uptake. Depletion of plasma membrane cholesterol reversibly inhibited oleate uptake by adipocytes without altering the amount or the cell surface distribution of either caveolin-1 or CD36. Cholesterol levels thus regulate fatty acid uptake by adipocytes via a pathway that does not involve altered cell surface localization of caveolin-1 or CD36.
Covey
Scott D
SD
Department of Biochemistry and Biomedical Sciences, 1200 Main St. W. Hamilton, Ont., Canada L8N 3Z5.
Brunet
Rachelle H
RH
Gandhi
Shephali G
SG
McFarlane
Nicole
N
Boreham
Douglas R
DR
Gerber
Gerhard E
GE
Trigatti
Bernardo L
BL
D016475:3T3 Cells; D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D018955:CD36 Antigens / Q000378:metabolism*; D051242:Caveolin 1 / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism*; D051379:Mice; D019301:Oleic Acid / Q000378:metabolism*
D018955:CD36 Antigens; D051242:Caveolin 1; D019301:Oleic Acid; D002784:Cholesterol