Evidence for interstate transmission and increase in prevalence of bovine group B rotavirus strains with a novel VP7 genotype among diarrhoeic calves in Eastern and Northern states of India.
During a surveillance study (2003-2005) in a cattle market in Kolkata city, state of West Bengal, Eastern India, 34 (13.0%) of 260 calves with diarrhoea were positive for group B rotaviruses (GBR) by RNA electrophoresis in polyacrylamide gels. Analysis of the partial VP7 gene sequence of 28 of the 34 GBR strains revealed maximum identities (97.7-99.5% at nucleotide level and 97.8-100% at amino-acid level) with the novel bovine GBR 'Kolkata strains' reported in an earlier surveillance study (1.5%, n=192, 2001-2002) from the same cattle market, and shared low identities of 73.7-78.9% and 80.8-89.6%; 62.6-66.2% and 59.8-65.4%; 58.9-62.2% and 48.6-54.9% at nucleotide and amino-acid level with other bovine, human, and murine GBR. The GBR-infected calves were traced to districts in neighbouring states of West Bengal. Therefore, the present study reports a rapid increase in prevalence (13.0% in 2003-2005 against 1.5% in 2001-2002) of novel GBR strains among calves with diarrhoea, and provides evidence for interstate transmission of GBR.
Ghosh
S
S
Division of Virology, National Institute of Cholera and Enteric Diseases, Beliaghata, Kolkata, India.
Varghese
V
V
Sinha
M
M
Kobayashi
N
N
Naik
T N
TN
D000818:Animals; D000956:Antigens, Viral / Q000235:genetics*; D036022:Capsid Proteins / Q000235:genetics*; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000635:transmission* / Q000821:virology; D016000:Cluster Analysis; D003967:Diarrhea / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000821:virology; D005838:Genotype; D007194:India; D017720:Molecular Epidemiology; D010802:Phylogeny; D015995:Prevalence; D012367:RNA, Viral / Q000302:isolation & purification; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology / Q000635:transmission / Q000662:veterinary* / Q000821:virology; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D000956:Antigens, Viral; D036022:Capsid Proteins; D012367:RNA, Viral; C050779:VP7 protein, Rotavirus