title: Poly-L-lactic acid/hydroxyapatite hybrid membrane for bone tissue regeneration.
abstract: Poly-L-lactic acid (PLLA)/hydroxyapatite (HA) hybrid membranes were fabricated via electrospinning of the PLLA/HA dispersion for use in bone tissue regeneration. The structural properties and morphologies of PLLA and PLLA/HA hybrid membrane were investigated by measuring the Brunauer-Emmett-Teller specific surface area, observations of SEM, and TEM. The dispersion and integrating of HA nanoparticles in the hybrid membrane were studied by energy dispersion X-ray analysis and FTIR. The mechanical properties of PLLA/HA membrane were also measured by tensile tests. For exploring biological behaviors of the hybrid membrane, in vitro degradation tests were carried out. The osteoblast cell (MG-63) was cultured in PLLA/HA hybrid membrane extract containing medium; the cell adhesion and growth capability were investigated by SEM observation and MTT assay. HA nanoparticles were not only dispersed in the PLLA but also reacted with the functional group of PLLA, resulting in strong surface bonding and high tensile strength of hybrid membrane. The cell adhesion and growth on the PLLA/HA hybrid membrane were far better than those on the pure PLLA membrane, which proves that the PLLA/HA hybrid membrane can be one of the promising biomaterials for bone tissue regeneration.
authors.affiliation: The Key Laboratory of Beijing City on Preparation and Processing of Novel Polymer, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Sui
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Yang
authors.forename: Fang
authors.initials: F
authors.lastname: Mei
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Hu
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Chen
authors.forename: Xuliang
authors.initials: X
authors.lastname: Deng
authors.forename: Seungkon
authors.initials: S
authors.lastname: Ryu
mesh_terms: D001696:Biomechanical Phenomena; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000737:chemistry; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D004355:Drug Stability; D017886:Durapatite / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000737:chemistry; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008970:Molecular Weight; D010006:Osteoblasts / Q000166:cytology; D011091:Polyesters; D011108:Polymers / Q000737:chemistry; D013499:Surface Properties; D013718:Tensile Strength; D014961:X-Ray Diffraction
chemical_list: D018786:Bone Substitutes; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D017886:Durapatite