title: On-line automatic SPE-CE coupling for the determination of biological markers in urine.
abstract: Automatic SPE has been coupled on-line to CE by a transfer tube and the replenishment system of the CE instrument. The approach allows the target analytes (viz. creatinine, creatine, xanthine, hypoxanthine, uric acid, p-aminohippuric acid and ascorbic acid in urine samples) to be removed from the sample matrix, cleaned up, preconcentrated and injected into the capillary. The detection limits range between 0.14 and 4.50 microg/mL, the quantification limits between 0.45 and 15.0 microg/mL, and linear dynamic ranges - which include the reference healthy human values - from the quantification limits to 1332 microg/mL. The precision, expressed as RSD, ranges between 0.38 and 2.22% for repeatability and between 1.79 and 7.61% for within-laboratory reproducibility. The errors, expressed as RSD for all compounds, range between 0.20 and 6.90%. The time for automatic SPE and that necessary for the individual separation-detection of the target analytes are 13 and 12 min, respectively; the analysis frequency is 5 h(-1). The accuracy of the method and potential matrix effects were studied by using spiked samples and recoveries between 96.00 and 103.07 % were obtained. The proposed method was applied to samples from healthy young students.
authors.affiliation: Department of Analytical Chemistry, University of Córdoba, Córdoba, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Ruiz-Jiménez
authors.forename: José M
authors.initials: JM
authors.lastname: Mata-Granados
authors.forename: Maria Dolores
authors.initials: MD
authors.lastname: Luque de Castro
mesh_terms: D000293:Adolescent; D000328:Adult; D001205:Ascorbic Acid / Q000652:urine; D015415:Biomarkers / Q000652:urine*; D003401:Creatine / Q000652:urine; D003404:Creatinine / Q000652:urine; D019075:Electrophoresis, Capillary / Q000379:methods; D006801:Humans; D019271:Hypoxanthine / Q000652:urine; D052616:Solid Phase Extraction; D014527:Uric Acid / Q000652:urine; D019820:Xanthine / Q000652:urine; D010130:p-Aminohippuric Acid / Q000652:urine
chemical_list: D015415:Biomarkers; D019820:Xanthine; D014527:Uric Acid; D019271:Hypoxanthine; D003404:Creatinine; D003401:Creatine; D001205:Ascorbic Acid; D010130:p-Aminohippuric Acid