[Detection of anisodamine and its metabolites in rat feces by tandem mass spectrometry].
OBJECTIVE
To establish a LC-MS(n) method for the identification of anisodamine and its metabolites in rat feces.


METHODS
Feces samples were collected after single administration of 25 mg x kg(-1) anisodamine to rats, and dipped in water for 1 h. Samples were then extracted by ethyl acetate. The pretreated samples were separated on a reversed-phase C18 column using a mobile phase of methanol / 0.01% triethylamine (adjusted to pH 3.5 with formic acid) (60 : 40, v/v) and detected by LC-MS". Identification of the metabolites and elucidation of their structures were performed by comparing their changes in molecular masses (deltaM), retention-times and full scan MS(n) spectra with those of the parent drug and blank feces.


RESULTS
The parent drug and its seven metabolites (6beta-hydroxytropine, nor-6beta-hydroxytropine, aponoranisodamine, apoanisodamine, noranisodamine and hydroxyanisodamine, tropic acid) were found in rat feces.


CONCLUSIONS
This method is sensitive, rapid, simple, effective, and suitable for the rapid identification of drug and its metabolites in biologic samples.
Chen
Huai-Xia
HX
Hubei Province Key Lab of Bio-Technology of Traditional Chinese Medicine, Hubei University, Wuhan 430062, China.
Du
Peng
P
Han
Feng-Mei
FM
Chen
Yong
Y
D000818:Animals; D005243:Feces / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D012991:Solanaceous Alkaloids / Q000032:analysis* / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D012991:Solanaceous Alkaloids; C003922:anisodamine