title: Evaluation of the interaction of propranolol with 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) liposomes: the partitioning model.
abstract: The sorption behavior of the amine containing beta-receptor blocking agent propranolol (Ppn) in 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) vesicles was investigated. Both protonated and unprotonated Ppn were measured in the continuous phase after removal of the vesicles containing sorbed Ppn by centrifugation. In contrast, by analyzing the surface charge density, deduced from electrophoretic mobility measurements, only the sorbed protonated Ppn was determined. A partitioning model was used to describe the sorption behavior. Sensitivity analysis revealed that sufficiently reliable and independent parameters were obtained. The partition coefficient of the unprotonated Ppn was about 22 times higher than that of the protonated analogue. Statistical analysis revealed a significant increase in the intrinsic partition coefficients of both Ppn analogues with an increase in the salt concentration.
authors.affiliation: Particle and Interfacial Technology Group, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium. Jan.Cocquyt@Ugent.be
authors.forename: Jan
authors.initials: J
authors.lastname: Cocquyt
authors.forename: Pieter
authors.initials: P
authors.lastname: Saveyn
authors.forename: Paul
authors.initials: P
authors.lastname: Van der Meeren
authors.forename: Marcel
authors.initials: M
authors.lastname: De Cuyper
mesh_terms: D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008081:Liposomes; D008956:Models, Chemical; D015394:Molecular Structure; D011433:Propranolol / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D008081:Liposomes; D011433:Propranolol; D004134:Dimyristoylphosphatidylcholine