title: Mapping of QTLs for oral alcohol self-administration in B6.C and B6.I quasi-congenic RQI strains.
abstract: One strategy to identify neurochemical pathways of addiction is to map the relevant genes. In the present study we used 43 B6.C and 35 B6.I inbred RQI mouse strains, carrying <3% donor genome on C57BL/6ByJ background, for gene mapping. The strains were phenotyped for consumption of alcohol (12% v/v) in a two-bottle-choice paradigm, and genotyped for 396 microsatellite markers. The current mapping study extends our earlier experiment scanning five mouse chromosomes (Vadasz et al. (2000) Scanning of five chromosomes for alcohol consumption loci. Alcohol 22:25-34) to a whole-genome study, and discusses the differences and limitations. Data were analyzed with composite interval (CIM) and multiple interval (MIM) QTL mapping methods. CIM of B6.C strains detected significant QTLs on chrs. 6 and 12. A suggestive, but not significant, locus was detected in the B6.I strains on chr. 12. The best MIM model for B6.C strains confirmed one QTL on chr. 6 and one QTL on chr. 12, while the MIM model for the B6.I strains confirmed the suggestive locus on chr. 12. Some of the QTLs for alcohol consumption are new, while others confirm previously reported QTLs for alcohol preference, and alcohol acceptance.
authors.affiliation: Laboratory of Neurobehavioral Genetics, Nathan S. Kline Institute for Psychiatric Research, 140 Old Orangeburg Road, Orangeburg, NY 10962, USA. vadasz@nki.rfmh.org
authors.forename: Csaba
authors.initials: C
authors.lastname: Vadasz
authors.forename: Mariko
authors.initials: M
authors.lastname: Saito
authors.forename: Beatrix M
authors.initials: BM
authors.lastname: Gyetvai
authors.forename: Melinda
authors.initials: M
authors.lastname: Oros
authors.forename: Istvan
authors.initials: I
authors.lastname: Szakall
authors.forename: Krisztina M
authors.initials: KM
authors.lastname: Kovacs
authors.forename: Vidudala V T S
authors.initials: VV
authors.lastname: Prasad
authors.forename: Grant
authors.initials: G
authors.lastname: Morahan
authors.forename: Reka
authors.initials: R
authors.lastname: Toth
mesh_terms: D000428:Alcohol Drinking / Q000235:genetics*; D000818:Animals; D002874:Chromosome Mapping; D000431:Ethanol / Q000008:administration & dosage*; D014644:Genetic Variation; D008297:Male; D051379:Mice; D020297:Mice, Congenic; D008815:Mice, Inbred Strains / Q000235:genetics*; D010641:Phenotype; D040641:Quantitative Trait Loci; D012646:Self Administration
chemical_list: D000431:Ethanol