title: Development and validation of a liquid chromatographic method for the analysis of positron emission tomography radiopharmaceuticals with Ru(bpy)(3)(2+)-KMnO(4) chemiluminescence detection.
abstract: A sensitive, selective and rapid high-performance liquid chromatographic (HPLC) method with chemiluminescence (CL) detection was developed and validated for the analysis of positron emission tomography (PET) radiopharmaceuticals. This method is based on the CL reaction of PET compounds with tris(2,2'-bipyridyl)ruthenium(II) [Ru(bpy)(3)(2+)] and acidic potassium permanganate [KMnO(4)]. After optimization of the reaction conditions, 12 of the 14 PET compounds investigated could be successfully detected and showed good performance in terms of sensitivity, linearity and reproducibility. In particular, for compounds with a tertiary amine functional group, the limits of detection were ppb levels for a 20 microL injection volume. Finally, this method was used to determine PET compounds for calculating of specific radioactivity in pharmaceutical samples.
authors.affiliation: Molecular Imaging Center, National Institute of Radiological Sciences, Anagawa, Inage, Chiba, Japan. nakaor@nirs.go.jp
authors.forename: Ryuji
authors.initials: R
authors.lastname: Nakao
authors.forename: Kenji
authors.initials: K
authors.lastname: Furutsuka
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Suzuki
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry; D000588:Amines / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056831:Coordination Complexes; D017022:Flow Injection Analysis / Q000379:methods; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements / Q000379:methods; D049268:Positron-Emission Tomography / Q000379:methods; D011196:Potassium Permanganate / Q000737:chemistry*; D019275:Radiopharmaceuticals; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors; D017975:Ruthenium Compounds
chemical_list: D015082:2,2'-Dipyridyl; D000588:Amines; D056831:Coordination Complexes; D011196:Potassium Permanganate; D019275:Radiopharmaceuticals; D011933:Reagent Kits, Diagnostic; C547232:tris(2,2-bipyridine)-ruthenium(II); D017975:Ruthenium Compounds