Analytical aspects of plant metabolite profiling platforms: current standings and future aims.
Over the past years, metabolic profiling has been established as a comprehensive systems biology tool. Mass spectrometry or NMR spectroscopy-based technology platforms combined with unsupervised or supervised multivariate statistical methodologies allow a deep insight into the complex metabolite patterns of plant-derived samples. Within this review, we provide a thorough introduction to the analytical hard- and software requirements of metabolic profiling platforms. Methodological limitations are addressed, and the metabolic profiling workflow is exemplified by summarizing recent applications ranging from model systems to more applied topics.
Seger
Christoph
C
Institute of Pharmacy/Pharmacognosy, Center of Molecular Biosciences, University of Innsbruck, Innrain 52, A-6020 Innsbruck, Austria. christoph.seger@uibk.ac.at
Sturm
Sonja
S
D002845:Chromatography; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D010944:Plants / Q000378:metabolism*; D012106:Research / Q000639:trends; D012680:Sensitivity and Specificity
D010940:Plant Proteins