Human cellular prion protein hPrPC is sorted to the apical membrane of epithelial cells.
Propagation of the scrapie isoform of the prion protein (PrP(Sc)) depends on the expression of endogenous cellular prion (PrP(C)). During oral infection, PrP(Sc) propagates, by conversion of the PrP(C) to PrP(Sc), from the gastrointestinal tract to the nervous system. Intestinal epithelium could serve as the primary site for PrP(C) conversion. To investigate PrP(C) sorting in epithelia cells, we have generated both a green fluorescent protein (EGFP) or hemagglutinin (HA) tagged human PrP(C) (hPrP(C)). Combined molecular, biochemical, and single living polarized cell imaging characterizations suggest that hPrP(C) is selectively targeted to the apical side of Madin-Darby canine kidney (MDCKII) and of intestinal epithelia (Caco2) cells.
De Keukeleire
B
B
CEA Grenoble, UMR 5249, CEA/CNRS/Université Joseph Fourrier, Institut de Recherches en Technologies et Sciences pour le Vivant, Laboratoire de Chimie et Biologie des Métaux, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France.
Donadio
S
S
Micoud
J
J
Lechardeur
D
D
Benharouga
M
M
D000818:Animals; D016466:CHO Cells; D018938:Caco-2 Cells / Q000378:metabolism; D016764:Cell Polarity; D006224:Cricetinae; D003412:Cricetulus; D004285:Dogs; D004847:Epithelial Cells / Q000378:metabolism* / Q000648:ultrastructure*; D006801:Humans; D018621:PrPC Proteins / Q000096:biosynthesis / Q000378:metabolism*; D021381:Protein Transport
D018621:PrPC Proteins