[Expression of HIF1-alpha on myocardium and lung in rats model of asphyxia death].
OBJECTIVE
To investigate the expression of HIF1-alpha in heart and lung tissue died from asphyxia.


METHODS
The rats model of asphyxia death was constructed by hanging, different asphyxia groups and control group sets were made according the postmortem time (0,2,6,24 h), immunohistochemistry and half-quantitative RT-PCR methods were used to investigate expression of HIF1-alpha and mRNA changes on heart and lung tissue.


RESULTS
The positive staining of HIF1-alpha could be observed in the myocardium and lung tissue. Significant differences were found between the groups of asphyxia and their corresponding control group. HIF1-alpha expression was found in all the asphyxia groups while it was only expressed in the control groups of 2 h, 6 h and 24 h. Nucleic positive staining could be detected in all the asphyxia groups but none was found in the control groups. RT-PCR showed that the expression of mRNA between 0 h asphyxia group and 0 h control group were equal in both cardic muscle and lung, but elevated expression in groups of 2,6,24h compared to their control groups.


CONCLUSIONS
The nuclear positive staining of HIF1-alpha in heart and lung can be a special character of suffocation death.
Zhang
Geng-qian
GQ
Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China. zgengqian@163.com
Zhou
Bin
B
Du
Bing
B
Yang
Zhi-hui
ZH
Zhang
Bei-lei
BL
Zhu
Yin-hua
YH
Zhang
Lin
L
D000818:Animals; D001237:Asphyxia / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism*; D020925:Hypoxia-Ischemia, Brain / Q000209:etiology / Q000378:metabolism*; D007150:Immunohistochemistry; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D008297:Male; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014018:Tissue Distribution
D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger