A new colorimetric method for determining the isomerization activity of sucrose isomerase.
A new colorimetric method for determining the isomerization activity of sucrose isomerase was developed. This colorimetric method is based on the enzymatic reactions of invertase and glucose oxidase-peroxidase (GOD-POD). The main scheme for assaying sucrose isomerase activity is to degrade sucrose in the reaction mixture to glucose and fructose by invertase and to detect the concentration of glucose generated using GOD-POD. The concentrations of trehalulose and isomaltulose, reaction products of sucrose isomerase, are calculated from the concentration of glucose. This method allows rapid and accurate determination of the isomerization activity of sucrose isomerase without inhibition by hydrolysis activity.
Park
Sang-Eun
SE
Institute of Life Science and Resources, Graduate School of Biotechnology, KyungHee University, Yongin, Korea.
Cho
Mee-Hyun
MH
Lim
Jin Kyu
JK
Kim
Jong-Sang
JS
Kim
Jeong Hwan
JH
Kwon
Dae Young
DY
Park
Cheon-Seok
CS
D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003124:Colorimetry; D005964:Glucosyltransferases / Q000737:chemistry / Q000378:metabolism*; D007536:Isomerism; D007700:Kinetics; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000737:chemistry; D013395:Sucrose / Q000737:chemistry; D043324:beta-Fructofuranosidase / Q000737:chemistry
D013395:Sucrose; D010544:Peroxidases; C023057:glucose peroxidase; D005964:Glucosyltransferases; C116853:sucrose isomerase; D043324:beta-Fructofuranosidase