title: Survival change of ventral mesencephalon-derived progenitor cells after grafting into unilateral intact adult rat striatum.
abstract: Neural transplantation is one of the most promising treatments for neurodegenerative disorders. Survival rates of embryonic dopamine (DA) neurons following transplantation are low, between 2% and 20% in a number of animal models. To further establish survival changes of the transplanted gestational day 13.5 ventral mesencephalic (VM) cells into left intact adult rat striata so that design strategies of increasing survival of DA neurons, the tyrosine hydroxylase (TH) expression of VM-derived progenitor cells has been examined using immunohistochemistry and Western blot analysis. TH immunostaining revealed that the grafted VM cells developed to mature TH-positive neurons strongly at 3 weeks, peaked at 4 weeks, thereafter, gradually dropped following the degenerative expression of the grafted cells at both 5 and 6 weeks after transplantation. Western blot analysis also showed that the TH proteins were maximally expressed at 4 weeks post-grafting. Our finding suggested that the peak of surviving VM-derived TH positive cells occurred approximately 4 weeks after transplantation.
authors.affiliation: Division of Life Science, College of Natural Sciences and Applied Life Science (Brain Korea 21), Gyeongsang National University, Chinju, South Korea.
authors.forename: Moon Seok
authors.initials: MS
authors.lastname: Park
authors.forename: Hae Young
authors.initials: HY
authors.lastname: Lee
authors.forename: Shu Peng
authors.initials: SP
authors.lastname: Li
authors.forename: Phil-Ok
authors.initials: PO
authors.lastname: Koh
authors.forename: Jong Yoon
authors.initials: JY
authors.lastname: Bahk
authors.forename: Bong Chul
authors.initials: BC
authors.lastname: Chung
authors.forename: Myeong Ok
authors.initials: MO
authors.lastname: Kim
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002470:Cell Survival / Q000502:physiology; D003342:Corpus Striatum / Q000378:metabolism / Q000502:physiology*; D005260:Female; D007150:Immunohistochemistry; D008636:Mesencephalon / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000502:physiology*; D013997:Time Factors; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D014446:Tyrosine 3-Monooxygenase