Nano-manipulation of single DNA molecules based on atomic force microscopy.
Nano-manipulation of single atoms and molecules is a critical technique in nanoscience and nanotechnology. This paper will focus on the recent development of the manipulation of single DNA molecules based on atomic force microscopy (AFM) in our laboratory. Precise manipulation has been realized including varied manipulating modes such as "cutting", "pushing", "folding", "kneading", "picking up", "dipping", etc. The cutting accuracy is dominated by the size of the AFM tip, which is usually 10nm or less. Single DNA fragments can be cut and picked up and then amplified by single molecule PCR. Thus positioning isolation and sequencing can be performed.
Jun-Hong
Lu
L
Shanghai Institute of Applied Physics, the Chinese Academy of Sciences, Shanghai 201800.
Hai-Kuo
Li
L
Hong-Jie
An
A
Guo-Hua
Wang
W
Ying
Wang
W
Min-Qian
Li
L
Yi
Zhang
Z
Bin
Li
L
Jun
Hu
H