Cytocompatibility and blood compatibility of multifunctional fibroin/collagen/heparin scaffolds.
An applicable matrix used in tissue engineering should not only have suitable mechanical properties, porous structures and biocompatibility that facilitate the adhesion, growth and proliferation of tissue cells, but also have the ability to release bioactive factors to provide a more conducive and inductive environment for tissue growth. Because of the harsh preparation conditions and deficiency of mechanical properties, it is still difficult for fibroin and collagen matrices to possess these multifunctional properties. In this research, we successfully prepared fibroin/collagen hybrid scaffolds containing heparin that possess multifunctional properties under mild conditions. These scaffolds maintain outstanding mechanical properties and porous structures of fibroin-based scaffolds. Furthermore, the scaffolds keep the bioactivity of collagen, becoming delivering systems that release heparin slowly to make the scaffolds blood compatible. Compared with fibroin/collagen scaffolds, the scaffolds containing heparin further facilitate the growth of HepG2 cells since a more complex, dynamic environment was formed to promote the cell growth. Considering the mild aqueous preparation environment without crosslinking reaction, besides promoting the progress in blood contacting tissue engineering, our research has also opened a door to prepare various multifunctional fibroin/collagen hybrid matrices that combine the advantages of fibroin and collagen.
Lu
Qiang
Q
Laboratory of Advanced Materials, Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, People's Republic of China.
Zhang
Shenjia
S
Hu
Kun
K
Feng
Qingling
Q
Cao
Chuanbao
C
Cui
Fuzhai
F
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001696:Biomechanical Phenomena; D012831:Bombyx; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D002452:Cell Count; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003094:Collagen / Q000737:chemistry* / Q000648:ultrastructure; D005349:Fibroins / Q000737:chemistry* / Q000648:ultrastructure; D005612:Freeze Drying; D005782:Gels / Q000737:chemistry; D006493:Heparin / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D006863:Hydrogen-Ion Concentration; D019476:Insect Proteins / Q000737:chemistry* / Q000648:ultrastructure; D007700:Kinetics; D008113:Liver Neoplasms / Q000473:pathology; D009391:Nephelometry and Turbidimetry; D016062:Porosity; D011517:Prothrombin Time; D013918:Thrombin Time; D023822:Tissue Engineering; D014867:Water / Q000737:chemistry
D001672:Biocompatible Materials; D005782:Gels; D019476:Insect Proteins; D014867:Water; D006493:Heparin; D003094:Collagen; D005349:Fibroins