Localization of protein secretion in fungal colonies using a novel culturing technique; the ring-plate system.
A novel culturing technique, called the ring-plate system, is described. Fungal colonies are grown on a polycarbonate membrane placed on a plate with 6 ring-shaped wells that are filled with liquid medium. This culturing technique enables simultaneous monitoring of environmental conditions and secretion in different parts of the fungal colony.
Levin
Ana M
AM
Microbiology, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
de Vries
Ronald P
RP
Wösten
Han A B
HA
D001234:Aspergillus niger / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000378:metabolism*; D008828:Microbiological Techniques / Q000379:methods*
D005656:Fungal Proteins