Realizing a two-dimensional ordered array of ferritin molecules directly on a solid surface utilizing carbonaceous material affinity peptides.
A two-dimensional hexagonally close-packed (2D-HCP) array of ferritin molecules with a nanoparticle core was fabricated directly on a carbonaceous solid substrate by genetically modifying the outer surface of the ferritin with carbonaceous materials-specific binding peptides. The displayed peptides endowed ferritins with a specific protein-substrate interaction and masked the strong nonspecific interaction. The specific interaction was weak enough to allow ferritins to be rearranged as well as an attractive protein-protein interaction that could be adjusted by selecting the buffer conditions. This method not only produced 2D-HCP arrays of ferritin but also 2D-ordered arrays of independent inorganic nanoparticles after protein elimination that can be applied to floating gate memories.
Matsui
Takuro
T
Advanced Technology Research Laboratories, Matsushita Electric Industrial Co., Ltd. (Panasonic), 3-4 Hikaridai, Seika-cho, Soraku-gun, Kyoto 619-0237, Japan.
Matsukawa
Nozomu
N
Iwahori
Kenji
K
Sano
Ken-Ichi
K
Shiba
Kiyotaka
K
Yamashita
Ichiro
I
D000595:Amino Acid Sequence; D005293:Ferritins / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure*; D010455:Peptides / Q000737:chemistry*
D010455:Peptides; D005293:Ferritins