title: Production of aminoglycosides in non-aminoglycoside producing Streptomyces lividans TK24.
abstract: The pRBM4 cosmid, which harbors a putative cluster of genes spanning a 31.8-kb chromosomal region of the ribostamycin producer Streptomyces ribosidificus ATCC 21294, was heterologously expressed in Streptomyces lividans TK24. ESI-MS/MS, HPLC, and LC-ESI MS analyses showed that the transformation gave rise to ribostamycin production in various culture broths. This is the first report of heterologous aminoglycoside production.
authors.affiliation: Institute of Biomolecule Reconstruction (IBR), Department of Pharmaceutical Engineering, Sun Moon University, #100, Kalsan-ri, Tangjeong-myeon, Asansi, Chungnam 336-708, Republic of Korea.
authors.forename: Bimala
authors.initials: B
authors.lastname: Subba
authors.forename: Nagendra Prasad
authors.initials: NP
authors.lastname: Kurumbang
authors.forename: Young Soo
authors.initials: YS
authors.lastname: Jung
authors.forename: Yeo Joon
authors.initials: YJ
authors.lastname: Yoon
authors.forename: Hei Chan
authors.initials: HC
authors.lastname: Lee
authors.forename: Kwangkyoung
authors.initials: K
authors.lastname: Liou
authors.forename: Jae Kyung
authors.initials: JK
authors.lastname: Sohng
mesh_terms: D000617:Aminoglycosides / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D001671:Biochemistry / Q000379:methods; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003360:Cosmids; D015195:Drug Design; D008956:Models, Chemical; D008968:Molecular Conformation; D010957:Plasmids / Q000378:metabolism; D012271:Ribostamycin / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D048170:Streptomyces lividans / Q000378:metabolism*
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D012271:Ribostamycin