Photodynamic inactivation of in vitro bacterial cultures from pressure ulcers.
OBJECTIVE
To evaluate in vitro the antibacterial effect of diode laser light of wavelength 650 nm, in association with the photosensitive substance toluidine blue, on the bacteria in infected skin ulcers.


METHODS
Samples were collected by means of swabs containing a medium for transporting infected material from skin ulcers. The material was inoculated into culturing medium containing azide blood agar for the growth of Gram-positive bacteria, and MacConkey agar for Gram-negative bacteria, and incubated for 48 hours. The results obtained from counting the colony-forming units were correlated and subjected to statistical analysis, adopting the significance level of p > or = 0.05.


RESULTS
From analysis of variance (ANOVA), the result for the general mean was p = 0.0215. Using the t test with post-hoc test, the result for TBO vs. Control was p = 0.0186, and for TBO + Laser vs. Control it was p = 0.0039.


CONCLUSIONS
There was a significant reduction in colony-forming units when the cultures were subjected to photodynamic therapy.
Carvalho
Paulo de Tarso Camillo de
Pde T
Department of Physiotherapy, UNIDERP, Brazil. ptpaulo@terra.com.br
Marques
Ana Paula da Costa
AP
Reis
Felipe Abdalla dos
FA
Belchior
Ana Carulina Guimarães
AC
Silva
Iandara Schettert
IS
Habitante
Carlos Alexandre
CA
Sussai
Daniela Aparecida
DA
D000704:Analysis of Variance; D015169:Colony Count, Microbial; D006090:Gram-Negative Bacteria / Q000528:radiation effects*; D006094:Gram-Positive Bacteria / Q000528:radiation effects*; D006801:Humans; D054023:Lasers, Semiconductor / Q000627:therapeutic use*; D028022:Low-Level Light Therapy; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology* / Q000627:therapeutic use; D003668:Pressure Ulcer / Q000188:drug therapy / Q000382:microbiology / Q000532:radiotherapy*; D014048:Tolonium Chloride / Q000494:pharmacology
D017319:Photosensitizing Agents; D014048:Tolonium Chloride