title: Characterisation of the subtelomeric regions of Giardia lamblia genome isolate WBC6.
abstract: Giardia trophozoites are polyploid and have five chromosomes. The chromosome homologues demonstrate considerable size heterogeneity due to variation in the subtelomeric regions. We used clones from the genome project with telomeric sequence at one end to identify six subtelomeric regions in addition to previously identified subtelomeric regions, to study the telomeric arrangement of the chromosomes. The subtelomeric regions included two retroposons, one retroposon pseudogene, and two vsp genes, in addition to the previously identified subtelomeric regions that include ribosomal DNA repeats. The presence of vsp genes in a subtelomeric region suggests that telomeric rearrangements may contribute to the generation of vsp diversity. These studies of the subtelomeric regions of Giardia may contribute to our understanding of the factors that maintain stability, while allowing diversity in chromosome structure.
authors.affiliation: Department of Immunobiology, University of Arizona College of Medicine, 1501 N. Campbell, Tucson, AZ 85724-5049, USA.
authors.forename: Anjali
authors.initials: A
authors.lastname: Prabhu
authors.forename: Hilary G
authors.initials: HG
authors.lastname: Morrison
authors.forename: Charles R
authors.initials: CR
authors.lastname: Martinez
authors.forename: Rodney D
authors.initials: RD
authors.lastname: Adam
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics; D002875:Chromosomes / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D017125:Genes, Protozoan / Q000235:genetics; D018503:Genome, Protozoan / Q000235:genetics*; D016829:Giardia lamblia / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics; D009693:Nucleic Acid Hybridization / Q000379:methods; D015800:Protozoan Proteins / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D018626:Retroelements / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D016615:Telomere / Q000235:genetics*
chemical_list: D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D008565:Membrane Proteins; D015800:Protozoan Proteins; D018626:Retroelements; C103927:Vsp protein, Giardia intestinalis