Effects of cadium, zinc and lead on soil enzyme activities.
Heavy metal (HM) is a major hazard to the soil-plant system. This study investigated the combined effects of cadium (Cd), zinc (Zn) and lead (Pb) on activities of four enzymes in soil, including calatase, urease, invertase and alkalin phosphatase. HM content in tops of canola and four enzymes activities in soil were analyzed at two months after the metal additions to the soil. Pb was not significantly inhibitory than the other heavy metals for the four enzyme activities and was shown to have a protective role on calatase activity in the combined presence of Cd, Zn and Pb; whereas Cd significantly inhibited the four enzyme activities, and Zn only inhibited urease and calatase activities. The inhibiting effect of Cd and Zn on urease and calatase activities can be intensified significantly by the additions of Zn and Cd. There was a negative synergistic inhibitory effect of Cd and Zn on the two enzymes in the presence of Cd, Zn and Pb. The urease activity was inhibited more by the HM combinations than by the metals alone and reduced approximately 20%-40% of urease activity. The intertase and alkaline phosphatase activities significantly decreased only with the increase of Cd concentration in the soil. It was shown that urease was much more sensitive to HM than the other enzymes. There was a obvious negative correlation between the ionic impulsion of HM in soil, the ionic impulsion of HM in canola plants tops and urease activity. It is concluded that the soil urease activity may be a sensitive tool for assessing additive toxic combination effect on soil biochemical parameters.
Yang
Zhi-xin
ZX
College of Resource and Environment, China Agricultural University, Beijing 100094, China. yangzhixin@126.com
Liu
Shu-qing
SQ
Zheng
Da-wei
DW
Feng
Sheng-dong
SD
D000469:Alkaline Phosphatase / Q000378:metabolism; D001937:Brassica / Q000187:drug effects* / Q000254:growth & development; D002374:Catalase / Q000037:antagonists & inhibitors / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism*; D019216:Metals, Heavy / Q000378:metabolism* / Q000633:toxicity; D012987:Soil / Q000032:analysis*; D014510:Urease / Q000037:antagonists & inhibitors / Q000378:metabolism; D043324:beta-Fructofuranosidase / Q000378:metabolism
D004798:Enzymes; D019216:Metals, Heavy; D012987:Soil; D002374:Catalase; D000469:Alkaline Phosphatase; D043324:beta-Fructofuranosidase; D014510:Urease