A derepression system based on the Bacillus subtilis sporulation pathway offers dynamic control of heterologous gene expression.
By rewiring the sporulation gene-regulatory network of Bacillus subtilis, we generated a novel expression system relying on derepression. The gene of interest is placed under the control of the abrB promoter, which is active only when Spo0A is absent, and Spo0A is controlled via an IPTG (isopropyl-beta-d-thiogalactopyranoside)-inducible promoter.
Nijland
Reindert
R
Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands.
Veening
Jan-Willem
JW
Kuipers
Oscar P
OP
D001412:Bacillus subtilis / Q000235:genetics* / Q000502:physiology; D001426:Bacterial Proteins / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D005434:Flow Cytometry; D015964:Gene Expression Regulation, Bacterial; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D013171:Spores, Bacterial / Q000502:physiology; D014121:Toxoids / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics
C060762:AbrB protein, Bacillus subtilis; D001426:Bacterial Proteins; C035454:Clostridium perfringens toxoid; D004268:DNA-Binding Proteins; D014121:Toxoids; D014157:Transcription Factors; D007544:Isopropyl Thiogalactoside