title: Crystallization of the archaeal transcription termination factor NusA: a significant decrease in twinning under microgravity conditions.
abstract: The transcription termination factor NusA from Aeropyrum pernix was crystallized using a counter-diffusion technique in both terrestrial and microgravity environments. Crystallization under microgravity conditions significantly reduced the twinning content (1.0%) compared with terrestrially grown crystals (18.3%) and improved the maximum resolution from 3.0 to 2.29 A, with similar unit-cell parameters. Based on a comparison of the crystal parameters, the effect of microgravity on protein crystallization is discussed.
authors.affiliation: Confocal Science Inc., Wakamatsu Building 7F, 3-3-6 Nihonbashi Hon-cho, Chuo-ku, Tokyo 103-0023, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Tanaka
authors.forename: Takashi
authors.initials: T
authors.lastname: Umehara
authors.forename: Koji
authors.initials: K
authors.lastname: Inaka
authors.forename: Sachiko
authors.initials: S
authors.lastname: Takahashi
authors.forename: Rie
authors.initials: R
authors.lastname: Shibata
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Bessho
authors.forename: Masaru
authors.initials: M
authors.lastname: Sato
authors.forename: Shigeru
authors.initials: S
authors.lastname: Sugiyama
authors.forename: Emiko
authors.initials: E
authors.lastname: Fusatomi
authors.forename: Takaho
authors.initials: T
authors.lastname: Terada
authors.forename: Mikako
authors.initials: M
authors.lastname: Shirouzu
authors.forename: Satoshi
authors.initials: S
authors.lastname: Sano
authors.forename: Moritoshi
authors.initials: M
authors.lastname: Motohara
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Tanaka
authors.forename: Akiko
authors.initials: A
authors.lastname: Tanaka
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yokoyama
mesh_terms: D041522:Aeropyrum / Q000737:chemistry*; D019843:Archaeal Proteins / Q000737:chemistry*; D018360:Crystallography, X-Ray; D010445:Peptide Elongation Factors / Q000737:chemistry*; D014157:Transcription Factors / Q000737:chemistry*
chemical_list: D019843:Archaeal Proteins; D010445:Peptide Elongation Factors; D014157:Transcription Factors