Scanning holographic microscopy with resolution exceeding the Rayleigh limit of the objective by superposition of off-axis holograms.
We present what we believe to be a new application of scanning holographic microscopy to superresolution. Spatial resolution exceeding the Rayleigh limit of the objective is obtained by digital coherent addition of the reconstructions of several off-axis Fresnel holograms. Superresolution by holographic superposition and synthetic aperture has a long history, which is briefly reviewed. The method is demonstrated experimentally by combining three off-axis holograms of fluorescent beads showing a transverse resolution gain of nearly a factor of 2.
Indebetouw
Guy
G
Department of Physics, Virginia Tech, Blacksburg, Virginia 24061-0435, USA. gindebet@vt.edu
Tada
Yoshitaka
Y
Rosen
Joseph
J
Brooker
Gary
G
D006696:Holography / Q000295:instrumentation / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D008962:Models, Theoretical