Microfluidic device for the detection of glucose using a micro direct methanol fuel cell as an amperometric detection power source.
We designed and prepared a novel microbiosensing system consisting of a microbioreactor fabricated using photosensitive sheets intercalated between Pyrex wafers as a dam structure, together with a micro fuel cell as a power source device between the electrodes for amperometric detection. The dam structure retains enzyme (glucose oxidase, GOx)-immobilized microbeads in a microchannel. Microelectrodes are used as an integrated detector within a microchannel located downstream of the dam structure, and these are used to detect the oxidation current of hydrogen peroxide produced from a glucose sample and GOx. A micro direct methanol fuel cell (mu-DMFC, i.d. 500 microm) was fabricated on a polymeric substrate and was used to supply a potential for the electrochemical detector. In this case, two mu-DMFCs were stacked on one substrate to increase the voltage for the oxidation of hydrogen peroxide. A linear response curve was obtained in range from 0.1 to 10 mM glucose for the designed microbiosensing system. These results show that a microfluidic biosensing system designed with a mu-DMFC device is useful and has the potential to assist minuaturization and simplification of the sensing system, in addition to increasing disposability of the device.
Ito
Takeshi
T
Kanagawa Industrial Technology Center, Shimoimaizumi, Ebina, Kanagawa 243-0435, Japan. taito@kanagawa-iri.go.jp
Kunimatsu
Masayuki
M
Kaneko
Satoru
S
Ohya
Seishiro
S
Suzuki
Koji
K
D019149:Bioreactors; D011211:Electric Power Supplies; D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry; D000432:Methanol / Q000737:chemistry*; D008839:Microelectrodes; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors
D004800:Enzymes, Immobilized; D005949:Glucose Oxidase; D005947:Glucose; D000432:Methanol