title: Novel cell culture device enabling three-dimensional cell growth and improved cell function.
abstract: A better understanding of cell biology and cell-cell interactions requires three-dimensional (3-D) culture systems that more closely represent the natural structure and function of tissues in vivo. Here, we present a novel device that provides an environment for routine 3-D cell growth in vitro. We have developed a thin membrane of polystyrene scaffold with a well defined and uniform porous architecture and have adapted this material for cell culture applications. We have exemplified the application of this technology by growing HepG2 liver cells on 2- and 3-D substrates. The performance of HepG2 cells grown on scaffolds was significantly enhanced compared to functional activity of cells grown on 2-D plastic. The incorporation of thin membranes of porous polystyrene to create a novel device has been successfully demonstrated as a new 3-D cell growth technology for routine use in cell culture.
authors.affiliation: School of Biological and Biomedical Science, Durham University, South Road, Durham, UK.
authors.forename: Maria
authors.initials: M
authors.lastname: Bokhari
authors.forename: Ross J
authors.initials: RJ
authors.lastname: Carnachan
authors.forename: Neil R
authors.initials: NR
authors.lastname: Cameron
authors.forename: Stefan A
authors.initials: SA
authors.lastname: Przyborski
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000378:metabolism; D018929:Cell Culture Techniques / Q000295:instrumentation*; D045744:Cell Line, Tumor; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D011137:Polystyrenes; D012709:Serum Albumin / Q000096:biosynthesis
chemical_list: D011137:Polystyrenes; D012709:Serum Albumin