title: Fluorescent Detection of Oral Pathogens by a Solid-Phase Immunoassay on PDMS.
abstract: We have developed an array of sensors for the oral pathogen Streptococcus mutans (S. mutans) using an enzymelinked linked immunosorbent assay (ELISA) on a polydimethylsiloxane (PDMS) device. The model bacterial analyte, S. mutans, has been implicated in the initiation and progression of dental caries. The PDMS was modified with 3-aminopropyltriethoxysilance (APTS) and glutaraldehyde to covalently crosslink monoclonal anti-S. mutans immunoglobulin G (IgG) to the sensor surface. Successful IgG immobilization was verified by AFM and fluorescence imaging. Colloidal bacteria were captured on the sensor surface and labeled with immuno-active quantum dots (QDs), whose fluorescence was excited by an LED and detected by a CCD. The system was capable of detecting S. mutans concentrations as low as 6 106cells/ml in a 20 μl sample. This work represents a stable foundation for the development of a chair side diagnostic system capable of specific and sensitive detection of pathogens directly from oral fluid.
authors.affiliation: Dept. of Bioengineering, UCLA, Los Angeles, CA 90095 USA.
authors.forename: A
authors.initials: A
authors.lastname: Fung
authors.forename: C-Y
authors.initials: CY
authors.lastname: Yang
authors.forename: S
authors.initials: S
authors.lastname: Freire
authors.forename: C
authors.initials: C
authors.lastname: Montemagno
authors.forename: B
authors.initials: B
authors.lastname: Brough
authors.forename: C-M
authors.initials: CM
authors.lastname: Ho
authors.forename: F
authors.initials: F
authors.lastname: Gu
authors.forename: W
authors.initials: W
authors.lastname: Shi