title: [Polymorphism of killer cell immunoglobulin-like receptor 2DL3 gene in Zhejiang Han population].
abstract: OBJECTIVE
  To analyze the allelic polymorphism of killer cell immunoglobulin-like receptor 2DL3 (KIR2DL3) gene in Zhejiang Han population.
  
  
  METHODS
  Allelic of KIR2DL3 was detected by PCR sequence based typing (PCR-SBT) method. Two specific fragments of KIR2DL3 were amplified using different primers and sequenced for exons 4,5,7,8,9 of KIR2DL3. All the samples would be assigned to KIR2DL3 allele according to the polymorphism site patterns.
  
  
  RESULTS
  Two KIR2DL3 alleles were observed, with KIR2DL3*001 having higher frequency, 0.77.
  
  
  CONCLUSIONS
  The PCR-SBT method is reliable and there are distinctive frequencies of KIR2DL3 alleles in Zhejiang Han population.
authors.affiliation: Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, Zhejiang, 310006 PR China.
authors.forename: Fa-ming
authors.initials: FM
authors.lastname: Zhu
authors.forename: Kan
authors.initials: K
authors.lastname: Jiang
authors.forename: Qin-feng
authors.initials: QF
authors.lastname: Lv
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Ji
authors.initials: J
authors.lastname: He
authors.forename: Xian-guo
authors.initials: XG
authors.lastname: Xu
authors.forename: Li-xing
authors.initials: LX
authors.lastname: Yan
mesh_terms: D000483:Alleles; D001483:Base Sequence; D002681:China; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D054344:Receptors, KIR2DL3 / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D054344:Receptors, KIR2DL3