In vitro interaction of poliovirus with cytoplasmic dynein.
OBJECTIVE
Poliovirus (PV) enters the host by the oral route and can infect the central nervous system (CNS) by two mechanisms: crossing the blood-brain barrier and traveling along the nerves from the muscle to the spinal cord. In the latter mechanism, the PV receptor, CD155, and the motor protein, dynein, have been implicated in the transport of PV to the CNS. In this work we analyzed the possible interaction of PV with dynein.


METHODS
PV was bound to a Sepharose 4B beads and they were used to analyze the interaction of PV with cytoplasmic proteins from neuroblastoma cells by affinity chromatography and Western blot.


RESULTS
The interaction with cytoplasmic dynein was observed only when the Sepharose beads bound to PV were used and not in the control ones, where proteins from uninfected cells were coupled.


CONCLUSIONS
These preliminary results open the possibility that PV uses the dynein directly in its retrograde axonal transport.
González Durán
Elizabeth
E
Programa Institucional de Biomedicina Molecular, Escuela Nacional de Medicina y Homeopatía del Instituto Politécnico Nacional, Mexico D.F., Mexico.
del Angel
Rosa M
RM
Salas Benito
Juan Santiago
JS
D001370:Axonal Transport; D015153:Blotting, Western; D002460:Cell Line; D002846:Chromatography, Affinity; D004398:Dyneins / Q000378:metabolism*; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D017955:Poliovirus / Q000502:physiology*; D011485:Protein Binding; D011991:Receptors, Virus / Q000378:metabolism; D014764:Viral Proteins / Q000378:metabolism
D008565:Membrane Proteins; D011991:Receptors, Virus; D014764:Viral Proteins; C066100:poliovirus receptor; D004398:Dyneins