title: Hybrid bioelectrocatalyst for hydrogen peroxide reduction: immobilization of enzyme within organic-inorganic film of structured Prussian Blue and PEDOT.
abstract: Fabrication of structured film (on glassy carbon substrate) composed of compact Prussian Blue (that has been prepared by alternate immersions and through assembling within ultra-thin layers of 4(pyrrole-1-yl)-benzoic acid, PPyBA) and poly(3,4-ethylendioxythiophene), PEDOT, is described. This functionalized film has been characterized by fast charge propagation, and it has served as a redox conducting template for permanent attachment of a model enzyme, horseradish peroxidase, HRP. The resulting organic-inorganic system acts as an effective hybrid bioelectrocatalyst for electroreduction of hydrogen peroxide, a model reactant for biosensors and biofuel cells. Among important issues are rigidity, permanence of enzyme attachment, morphology, hydrophilicity, and attractive mediating capabilities of the PEDOT-stabilized Prussian Blue based structured film.
authors.affiliation: Department of Chemistry, University of Warsaw, Pasteura 1, PL-02-093 Warsaw, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Ernst
authors.forename: Oktawian
authors.initials: O
authors.lastname: Makowski
authors.forename: Barbara
authors.initials: B
authors.lastname: Kowalewska
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Miecznikowski
authors.forename: Pawel J
authors.initials: PJ
authors.lastname: Kulesza
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry*; D002244:Carbon / Q000737:chemistry; D002384:Catalysis; D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005295:Ferrocyanides / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000737:chemistry*; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D004800:Enzymes, Immobilized; D005295:Ferrocyanides; D011108:Polymers; C121383:poly(3,4-ethylene dioxythiophene); D002244:Carbon; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; C000170:ferric ferrocyanide