Sequence and annotation of the 288-kb ATCV-1 virus that infects an endosymbiotic chlorella strain of the heliozoon Acanthocystis turfacea.
Acanthocystis turfacea chlorella virus (ATCV-1), a prospective member of the family Phycodnaviridae, genus Chlorovirus, infects a unicellular, eukaryotic, chlorella-like green alga, Chlorella SAG 3.83, that is a symbiont in the heliozoon A. turfacea. The 288,047-bp ATCV-1 genome is the first virus to be sequenced that infects Chlorella SAG 3.83. ATCV-1 contains 329 putative protein-encoding and 11 tRNA-encoding genes. The protein-encoding genes are almost evenly distributed on both strands and intergenic space is minimal. Thirty-four percent of the viral gene products resemble entries in the public databases, including some that are unexpected for a virus. For example, these unique gene products include ribonucleoside-triphosphate reductase, dTDP-d-glucose 4,6 dehydratase, potassium ion transporter, aquaglyceroporin, and mucin-desulfating sulfatase. Comparison of ATCV-1 protein-encoding genes with the prototype chlorella virus PBCV-1 indicates that about 80% of the ATCV-1 genes are present in PBCV-1.
Fitzgerald
Lisa A
LA
Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.
Graves
Michael V
MV
Li
Xiao
X
Hartigan
James
J
Pfitzner
Artur J P
AJ
Hoffart
Ella
E
Van Etten
James L
JL
D000818:Animals; D001483:Base Sequence; D002708:Chlorella / Q000502:physiology / Q000821:virology; D004260:DNA Repair; D004261:DNA Replication; D021901:DNA, Intergenic / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D004798:Enzymes / Q000235:genetics; D056890:Eukaryota / Q000382:microbiology / Q000502:physiology; D016679:Genome, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000378:metabolism; D017862:Phycodnaviridae / Q000235:genetics*; D012343:RNA, Transfer / Q000235:genetics; D017385:Sequence Homology; D013559:Symbiosis; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
D021901:DNA, Intergenic; D004279:DNA, Viral; D004798:Enzymes; D009711:Nucleotides; D014764:Viral Proteins; D012343:RNA, Transfer