title: [Vitro culture of blastocystis hominis in medium DMEM].
abstract: OBJECTIVE
  To establish the vitro culture of Blastocystis hominis (B. h) in medium DMEM for the further research on diagnosis, life cycle and pathogenicity of this intestinal protoza.
  
  
  METHODS
  The growth, reproduction and relevant factors of B . h under different culture conditions including sorts and concentrations of serum, pHs and number of inoculation were compared.
  
  
  RESULTS
  Conditions for the continuously anaerobic culture of B. h in medium DMEM were as follows: the number of inoculation were no less than 10(5) cells per tube, pHs ranged 7.0 - 8.0, concentrations of calf serum (or human serum and horse serum) ranged 10% - 30% , antibiotics and Amphotericin B should be added, subculturing could be choose at the each peaking-day 3,6 or 5 at 37 degrees C.
  
  
  CONCLUSIONS
  The medium DMEM could be used in diagnosis and continuously vitro culture for B. h.
authors.affiliation: Key Laboratory of Environment and Genes Related to Diseases, Ministery of Education, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.forename: Ji-ying
authors.initials: JY
authors.lastname: Qiao
authors.forename: Rong
authors.initials: R
authors.lastname: Da
authors.forename: Ya-qing
authors.initials: YQ
authors.lastname: Li
authors.forename: Fan-rong
authors.initials: FR
authors.lastname: Yao
mesh_terms: D000818:Animals; D016845:Blastocystis hominis / Q000254:growth & development* / Q000502:physiology; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D012098:Reproduction / Q000502:physiology
chemical_list: D003470:Culture Media