Amplified fragment length polymorphism (AFLP) is useful for finding markers associated with QTL for architectural trait in Hedysarum coronarium L.
Amplified fragment length polymorphism (AFLP) has been proposed as a valuable tool for finding molecular markers linked to QTL controlling architectural trait. Segregation of polymorphic AFLP fragments was followed in F2 offsprings Hedysarum coronarium derived from two native wild accesions crossing: Jebel Zit x El Haouaria, which had previously characterised by morphological and AFLP markers. A comparison of profiles from the morphologically contrasted parental individuals and F2 offspring has been assessed using five AFLP primer combinations. These generated 178 bands revealed by silver-stained denaturing polyacrylamide gels, of which 150 were polymorphic. Several AFLP markers appeared to be implied in the orthotropic form that can assist the selection and improve Hedysarum forage crop.
Marghali
S
S
Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie, Faculté des Sciences de Tunis, Campus universitaire, 2092 El Manar, Tunis, Tunisia.
Chennaoui
H
H
Bourguiba
H
H
Marrakchi
M
M
Trifi-Farah
N
N
D001483:Base Sequence; D003433:Crosses, Genetic; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D007887:Fabaceae / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D018522:Gravitropism / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques; D040641:Quantitative Trait Loci; D014416:Tunisia
D017931:DNA Primers; D018744:DNA, Plant