[Study on transdifferentiation of renal tubular cells in rat chronic renal interstitial fibrosis induced by Radix Aristolochiae Fangchi Extract].
OBJECTIVE
To investigate the relationship between renal tubular cells transdifferentiation and chronic renal interstitial fibrosis induced by Fangchi Extract in rat.


METHODS
The chronic renal interstitial fibrosis rat model was made by giving Radix Aristolochiae Fangchi extract (RAFE) and aristolichic acid (AA) respectively to rats through infusing stomach about 22 weeks discontinuously. Through immunnal histochemistry methods, investigating the expression of symbol proteins: Cytokine( CK) , alpha-Smooth muscle actin ( alpha-SMA) and Vimentin, and also the important fibrosis inducing factor-Transforming growth factor-beta (TGF-beta1 )on renal tubular cells.


RESULTS
In RAFE and AA Groups, the expression of CK on renal tubular cells is declined comparing with the Control Group, and the enhanced expression of alpha-SMA and Vimentin can be observed on tubular cells. The expression of TGF-beta1 on renal tubular cells stronglhy increased, too.


CONCLUSIONS
Part of the renal tubular cells was transdifferentiated into myofibroblasts. Renal tubular cells may participate the occurance of chronic renal interstitial fibrosis, TGF-beta1 may accelerate the transdifferentiation of tubular cells.
Zhou
Su-juan
SJ
Center for Health Food Evaluation, State Food and Drug Administration, Beijing 100061, China.
Du
Gui-you
GY
Zhao
Yong
Y
Cui
Hai-feng
HF
Wang
Xiu-rong
XR
Li
Li
L
Xiao
Yong-qing
YQ
D000199:Actins / Q000378:metabolism; D000818:Animals; D030022:Aristolochia / Q000737:chemistry*; D034341:Aristolochic Acids / Q000302:isolation & purification / Q000633:toxicity; D054338:Cell Transdifferentiation / Q000187:drug effects*; D016207:Cytokines / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000633:toxicity*; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D005260:Female; D005355:Fibrosis; D007674:Kidney Diseases / Q000139:chemically induced / Q000378:metabolism / Q000503:physiopathology; D007684:Kidney Tubules / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism
D000199:Actins; D034341:Aristolochic Acids; D016207:Cytokines; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D053773:Transforming Growth Factor beta1; D014746:Vimentin; C498862:Acta2 protein, rat