New small nuclear RNA gene-like transcriptional units as sources of regulatory transcripts.
By means of a computer search for upstream promoter elements (distal sequence element and proximal sequence element) typical of small nuclear RNA genes, we have identified in the human genome a number of previously unrecognized, putative transcription units whose predicted products are novel noncoding RNAs with homology to protein-coding genes. By elucidating the function of one of them, we provide evidence for the existence of a sense/antisense-based gene-regulation network where part of the polymerase III transcriptome could control its polymerase II counterpart.
Pagano
Aldo
A
Dipartimento di Oncologia Biologia e Genetica, Università di Genova, Genoa, Italy. aldo.pagano@istge.it
Castelnuovo
Manuele
M
Tortelli
Federico
F
Ferrari
Roberto
R
Dieci
Giorgio
G
Cancedda
Ranieri
R
D000818:Animals; D001483:Base Sequence; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics; D019295:Computational Biology; D005786:Gene Expression Regulation; D015894:Genome, Human / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008840:Microfilament Proteins / Q000235:genetics; D008957:Models, Genetic; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D009690:Nucleic Acid Conformation; D012320:RNA Polymerase III / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012342:RNA, Small Nuclear / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D016385:TATA Box / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics*
D002868:Chromosomal Proteins, Non-Histone; D008840:Microfilament Proteins; D012333:RNA, Messenger; D012342:RNA, Small Nuclear; C094772:centromere protein F; D012320:RNA Polymerase III