Peroxidation of mineral oil used in droplet culture is detrimental to fertilization and embryo development.
Unexpected deterioration of human embryos led us to an investigation of possible toxic factors in the oil covering culture medium. Elevated levels of peroxidation in the oil by oxygen exposure or unfavorable storage were found to be the main causes of toxicity.
Otsuki
Junko
J
Department of Biology, Ochanomizu University, Tokyo, Japan. midori@nagai-cl.com <midori@nagai-cl.com>
Nagai
Yasushi
Y
Chiba
Kazuyoshi
K
D001755:Blastocyst / Q000187:drug effects / Q000473:pathology; D018929:Cell Culture Techniques; D047108:Embryonic Development; D005260:Female; D005306:Fertilization / Q000187:drug effects / Q000502:physiology*; D005307:Fertilization in Vitro; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008899:Mineral Oil / Q000633:toxicity*; D011247:Pregnancy
D008899:Mineral Oil