title: [Production of alpha-, beta-, and lambda-interferons by epithelial and mononuclear cells during acute respiratory viral infection].
abstract: Role of several types of cells (human broncho-epithelial cells, BEAS-2B cell line, and mononuclear cells as model of macrophages) in production of alpha-, beta- and lambda-interferons during acute respiratory viral infection was studied. Kits for detection of these interferons by quantitative PCR assay has been developed. In human broncho-epithelial cells respiratory viruses induced statistically significant expression of alpha-interferon mRNA at 8 hours after infection, beta-interferon mRNA--at 24 hours after infection, IL-29 mRNA (lambda-interferon) - at 24 hours after infection, IL-28 mRNA (lambda-interferon) - at 8 and 24 hours after infection. In BEAS-2B cell line induction of alpha-interferon mRNA expression was observed at 8 hours after infection, beta-interferon mRNA expression - at 24 hours after infection, IL-29 mRNA (lambda-interferon) expression - at 8 and 24 hours after viral challenge. Production of beta- and lambda-interferons by ELISA at 24 hours after infection has been detected. When polymorphonuclear cells were challenged, induction of alpha-, beta-, and lambda-interferons expression was observed at 8 hours after infection. Production of alpha-, beta- and lambda-interferons has been detected by ELISA at 24 hours after infection by rhinovirus 16.
authors.forename: M R
authors.initials: MR
authors.lastname: Khaitov
authors.forename: V
authors.initials: V
authors.lastname: Laza-Stantca
authors.forename: M R
authors.initials: MR
authors.lastname: Edwards
authors.forename: S L
authors.initials: SL
authors.lastname: Johnston
mesh_terms: D001980:Bronchi / Q000276:immunology; D002460:Cell Line; D004847:Epithelial Cells / Q000276:immunology; D006801:Humans; D007372:Interferons / Q000096:biosynthesis* / Q000235:genetics; D008264:Macrophages / Q000276:immunology; D009975:Orthomyxoviridae / Q000276:immunology*; D009976:Orthomyxoviridae Infections / Q000276:immunology*; D010850:Picornaviridae Infections / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis; D012229:Rhinovirus / Q000276:immunology*; D013997:Time Factors
chemical_list: D012333:RNA, Messenger; D007372:Interferons