Reduction of saliva-promoted adhesion of Streptococcus mutans MT8148 and dental biofilm development by tragacanth gum and yeast-derived phosphomannan.
The aim of this study was to investigate materials which reduce saliva-promoted adhesion of Streptococcus mutans onto enamel surfaces, and their potential in preventing dental biofilm development. The effects of hydroxyapatite (HA) surface pretreatment with hydrophilic polysaccharides on saliva-promoted S. mutans adhesion in vitro and de novo dental biofilm deposition in vivo were examined. Saliva-promoted adhesion of S. mutans MT8148 was significantly reduced by pretreatment of the HA surface with tragacanth gum (TG) and yeast-derived phosphoglycans. Extracellular phosphomannan (PM) from Pichia capsulata NRRL Y-1842 and TG reduced biofilm development on lower incisors in plaque-susceptible rats when administered via drinking water at concentrations of 0.5% and 0.01%, respectively. The inhibitory effect of TG on de novo dental biofilm formation was also demonstrated when administered via mouthwash in humans. It is concluded that TG and yeast-derived PM have the potential for use as anti-adherent agents and are effective in reducing de novo dental biofilm formation.
Shimotoyodome
A
A
Biological Science Laboratories, Kao Corporation, Tokyo, Japan. shimotoyodome.akira@kao.co.jp
Kobayashi
H
H
Nakamura
J
J
Tokimitsu
I
I
Hase
T
T
Inoue
T
T
Matsukubo
T
T
Takaesu
Y
Y
D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D003773:Dental Plaque / Q000382:microbiology / Q000517:prevention & control; D006882:Hydroxyapatites; D008297:Male; D008351:Mannans / Q000096:biosynthesis / Q000494:pharmacology*; D010843:Pichia / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D012463:Saliva / Q000378:metabolism*; D013295:Streptococcus mutans / Q000502:physiology*; D014070:Tooth / Q000187:drug effects / Q000382:microbiology*; D014144:Tragacanth / Q000494:pharmacology*
D006882:Hydroxyapatites; D008351:Mannans; D014144:Tragacanth; C005437:phosphomannan