Effect of modified pectin molecules on the growth of bone cells.
The aim of this study was to investigate molecular candidates for bone implant nanocoatings, which could improve biocompatibility of implant materials. Primary rat bone cells and murine preosteoblastic MC3T3-E1 cells were cultured on enzymatically modified hairy regions (MHR-A and MHR-B) of apple pectins. MHRs were covalently attached to tissue culture polystyrene (TCPS) or glass. Uncoated substrata or bone slices were used as controls. Cell attachment, proliferation, and differentiation were investigated with fluorescence and confocal microscopy. Bone cells seem to prefer MHR-B coating to MHR-A coating. On MHR-A samples, the overall numbers as well as proportions of active osteoclasts were diminished compared to those on MHR-B, TCPS, or bone. Focal adhesions indicating attachment of the osteoblastic cells were detected on MHR-B and uncoated controls but not on MHR-A. These results demonstrate the possibility to modify surfaces with pectin nanocoatings.
Kokkonen
Hanna E
HE
Department of Anatomy and Cell Biology, University of Oulu, Oulu, Finland. hanna.kokkonen@oulu.fi
Ilvesaro
Joanna M
JM
Morra
Marco
M
Schols
Henk A
HA
Tuukkanen
Juha
J
D016475:3T3 Cells; D000818:Animals; D001842:Bone and Bones / Q000166:cytology*; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D027845:Malus / Q000737:chemistry; D051379:Mice; D010368:Pectins / Q000494:pharmacology*; D051381:Rats; D046509:Tissue Culture Techniques
D010368:Pectins