title: Investigation of protective effects of selenium and vitamin E against DNA oxidation, membrane damage and alteration of COMT metabolism in smoke-exposed male mice (Mus musculus, Balb/c).
abstract: In our study, the protective effects of vitamin E and Se (selenium) against cigarette smoke hazards on second-hand smoker (passive smoker) male mice (Balb/c) were investigated. Serum MDA levels in the smoke-exposed mice were found higher than serum MDA levels of control mice and Se- and vitamin E-treated mice. But, the MDA levels of smoke-exposed plus Se- and vitamin E-treated mice were found lower than MDA levels of smoke-exposed mice at the end of the three and five months. According to these results, application of vitamin E and Se, when given to smoke-exposed mice together, had an additive protective effect against cigarette smoke hazards (p < 0.05). Vitamin E also had protective effect on formation of 8-OHdG in smoke-exposed mice. The serum 8-OHdG amounts of smoke-exposed plus vitamin E-treated mice were found low, but the serum 8-OHdG amounts of smoke-exposed mice were found high. Also 8-OHdG levels in the serum of the smoke-exposed mice were increased which occurs as a result of DNA oxidation (p < 0.05). At the end of the three and five months, COMT (catechol-o-methyl transferase) activity of smoke-exposed mice livers were increased but, vitamin E and/or Se showed a significant protective effect on changing of COMT activity only at the end of the 5 months. Our results showed that MDA levels and 8-OHdG amounts were increased in the serum of smoke-exposed mice. On the other hand, vitamin E and Se had an additive protective effect against increasing MDA level. Also vitamin E had a protective effect against formation of 8-OHdG amounts and COMT activity alterations.
authors.affiliation: Biology Department, Art-Science Faculty, Akdeniz University, 07058 Antalya, Turkey.
authors.forename: K
authors.initials: K
authors.lastname: Fiskin
authors.forename: A
authors.initials: A
authors.lastname: Ozkan
authors.forename: A G
authors.initials: AG
authors.lastname: Ayhan
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology; D002394:Catechol O-Methyltransferase / Q000378:metabolism*; D004249:DNA Damage / Q000187:drug effects*; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood / Q000378:metabolism; D004357:Drug Synergism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D010084:Oxidation-Reduction; D012643:Selenium / Q000008:administration & dosage / Q000494:pharmacology*; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*; D014810:Vitamin E / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D014028:Tobacco Smoke Pollution; D014810:Vitamin E; D008315:Malondialdehyde; D000080242:8-Hydroxy-2'-Deoxyguanosine; D002394:Catechol O-Methyltransferase; D003849:Deoxyguanosine; D012643:Selenium