Development of a frit-free SPE-based in-column preconcentration system for capillary electrophoresis.
An in-capillary sample preconcentration strategy based on solid phase extraction (SPE) technology coupled with capillary electrophoresis (CE) has been developed taking advantage of both techniques (SPE and CE). An in-line frit-free preconcentration device for capillary electrophoresis containing MCX beads, obtained from the corresponding Waters OASIS cartridges, was prepared. The retention of the particles was based on the relative diameters of the particles, carefully selected, and the capillaries. An experimental preconcentration factor of 100 was found for the system. Conditions were optimised for 3-nitrotyrosine measurement in rat urine being 4.4 microM spiked in the urine the lowest value detectable.
Saavedra
L
L
Sección de Química Analítica, Facultad de Farmacia, Universidad San Pablo-CEU, Campus Montepríncipe, 28668 Boadilla del Monte, Madrid, Spain.
Maeso
N
N
Cifuentes
A
A
Barbas
C
C
D000818:Animals; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D010316:Particle Size; D051381:Rats; D052617:Solid Phase Microextraction / Q000295:instrumentation*; D013056:Spectrophotometry, Ultraviolet; D014443:Tyrosine / Q000031:analogs & derivatives / Q000652:urine
C002744:3-nitrotyrosine; D014443:Tyrosine