Molecular cloning and functional expression of soybean allene oxide synthases.
A plant allene oxide synthase (AOS) reacting with 13S-hydroperoxy-9Z,11E,15Z-octadecatrienoic acid (13-HPOT), a lipoxygenase product of alpha-linolenic acid, provides an allene oxide which functions as an intermediate for jasmonic acid (JA) synthesis, making AOS a key enzyme regulating the JA level in plants. Although AOSs in various plants have been investigated, there is only limited information about AOSs in soybean (Glycine max). In this study, we cloned and characterized two soybean AOSs, GmAOS1 and GmAOS2, sharing 95% homology in the predicted amino acid sequences. GmAOS1 and GmAOS2 were composed of 564 and 559 amino acids respectively, with predicted N-terminal chloroplast-targeting signal peptides. Both AOSs expressed in Escherichia coli were selective for 13S-hydroperoxides of alpha-linolenic and linoleic acids, suggesting the potential of GmAOS1 and GmAOS2 to contribute to JA synthesis. GmAOS1 and GmAOS2 were expressed in leaves, stems, and roots, suggesting broad distribution in a soybean plant.
Kongrit
Darika
D
Division of Applied Resources Chemistry, The United Graduate School of Agricultural Sciences, Tottori University, Koyama-minami, Tottori, Japan.
Jisaka
Mitsuo
M
Iwanaga
Chitose
C
Yokomichi
Hiroshi
H
Katsube
Takuya
T
Nishimura
Kohji
K
Nagaya
Tsutomu
T
Yokota
Kazushige
K
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D005784:Gene Amplification; D019746:Intramolecular Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics*; D007700:Kinetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D018515:Plant Leaves / Q000201:enzymology; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics; D018517:Plant Roots / Q000201:enzymology; D018547:Plant Stems / Q000201:enzymology; D018749:RNA, Plant / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000201:enzymology; D013025:Glycine max / Q000201:enzymology* / Q000235:genetics*
D018076:DNA, Complementary; D010940:Plant Proteins; D018749:RNA, Plant; D019746:Intramolecular Oxidoreductases; C021377:hydroperoxide isomerase