[Study on rat's DNA damage of p53 induced by chlorinated acetic acids of drinking water disinfection by-products].
OBJECTIVE
To study on the DNA damage of p53 induced by dichloroacetic acid(DCA) and trichloroacetic acid( TCA), approve their genotoxicity and discuss molecular mechanism of their carcinogenic action.


METHODS
Administered SD rats with DCA or TCA by i.p. injection, extracted DNA from rat's liver, and then used RDPCR to detect DNA damage of exon 70f p53 gene.


RESULTS
Two hybridization bands were detected in treated group induced by DCA. It was indicated that DCA can result in DNA damage of exon 7 of p53 gene of rat's liver tissue, and there were two broken sites. It was not detected damage of exon 7 of p53 gene of rat induced by TCA.


CONCLUSIONS
There may be the relationship between DCA carcinogenic action and the damage of p53. The result that the damage of p53 of target tissue detected by RDPCR was consistent well with rat carcinogenic test.
Yang
Yuan
Y
West China School of Public Health, Sichuan University, Chengdu 610041, China.
Heng
Zheng-chang
ZC
D000818:Animals; D004247:DNA / Q000187:drug effects; D004249:DNA Damage; D003999:Dichloroacetic Acid / Q000633:toxicity*; D005091:Exons / Q000187:drug effects; D016158:Genes, p53 / Q000187:drug effects*; D008297:Male; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley; D014238:Trichloroacetic Acid / Q000633:toxicity*
D014238:Trichloroacetic Acid; D004247:DNA; D003999:Dichloroacetic Acid