Design of sphingomonad-detecting probes for a DNA array, and its application to investigate the behavior, distribution, and source of Rhizospherous sphingomonas and other sphingomonads inhabiting an acid sulfate soil paddock in Kalimantan, Indonesia.
Throughout Central and South Kalimantan, Indonesia, strongly acidic soil (pH 2.1-3.7) is widely distributed, and the local acidic soil-tolerant plants, including local rice varieties, often possess sphingomonads in their rhizosphere and rhizoplane. To investigate the behavior of sphingomonads inhabiting the rhizosphere of such acid-tolerant plants, we designed 13 different DNA array probes (each of 72 mer) specific to a group of sphingomonads, using a hypervariable V6 region of the 16S rRNA gene. This DNA array system was used preliminarily for an analysis of microfloral dynamisms, particularly of sphingomonads, in acidic paddock ecosystems, and the results suggest that the acid-tolerant local rice shares rhizospherous sphingomonads with wild Juncus sp., a predominant weed that thrives in acidic paddocks during the off-season for rice farming. This tentative conclusion supports the bio-rationality of the traditional rice farming system with respect to functional rhizobacteria.
Hashidoko
Yasuyuki
Y
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan. yasu-h@abs.agr.hokudai.ac.jp
Kitagawa
Emiko
E
Iwahashi
Hitoshi
H
Purnomo
Erry
E
Hasegawa
Toshihiro
T
Tahara
Satoshi
S
D016956:Burkholderia cepacia / Q000737:chemistry / Q000235:genetics; D015342:DNA Probes / Q000737:chemistry*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007214:Indonesia; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology; D020578:Sphingomonas / Q000235:genetics*; D013431:Sulfates / Q000737:chemistry
D015342:DNA Probes; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012987:Soil; D013431:Sulfates