Determination of atenolol and metoprolol by capillary electrophoresis with tris(2,2'-bipyridyl)ruthenium(ii) electrochemiluminescence detection.
Capillary electrophoresis (CE) coupling with a tris(2,2'-bipyridyl)ruthenium(II) (Ru(bpy)(3)(2+)) electrochemiluminescence (ECL) detection technique was developed for the analysis of two beta-blockers, atenolol (AT) and metoprolol (ME). The parameters that influence the separation and detection, including the buffer pH and concentration, the separation voltage, the detection potential and Ru(bpy)(3)(2+) concentration, were optimized in detail. The calibration curve was linear over a concentration range of two or three orders of magnitude for the two beta-blockers. The detection limits for AT and ME were 0.075 and 0.005 microM (S/N = 3). The relative standard deviations (n = 8) of the ECL intensity and the migration time were 2.65 and 0.22% for AT, 2.82 and 0.34% for ME, respectively. The proposed method was applied to determine AT and ME in spiked urine samples; satisfactory results were obtained.
Huang
Jianshe
J
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, China.
Sun
Jinying
J
Zhou
Xiaoguang
X
You
Tianyan
T
D000319:Adrenergic beta-Antagonists / Q000032:analysis*; D001262:Atenolol / Q000032:analysis*; D002021:Buffers; D002138:Calibration; D004563:Electrochemistry; D019075:Electrophoresis, Capillary; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements / Q000379:methods*; D008790:Metoprolol / Q000032:analysis*; D009942:Organometallic Compounds / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
D000319:Adrenergic beta-Antagonists; D002021:Buffers; D009942:Organometallic Compounds; C415768:tris(2,2'-bipyridyl)ruthenium(II); D001262:Atenolol; D008790:Metoprolol