[Activity changes of protein kinase C in effect of interferon-gamma on wound healing and cicatrisation].
OBJECTIVE
To study the roles of protein kinase C (PKC) in effect of interferon-gamma (IFN-gamma) on wound healing and cicatrization.


METHODS
IFN-gamma was applied on the wound and into the scar tissues of rabbit ear before or after wound healing. PKC activities in the tissues from 0, 3, 6 d, 11-16 d post-wounding and from 14, 30 and 45d post-epithelization were measured by phosphorus (32p) incorporation. The time of wound epithelization and scar changes were also observed.


RESULTS
The PKC activity in granulation tissue, wound margin tissue and scar tissue elevated obviously in comparing with that of normal skin (P < 0.01). IFN-gamma did not change PKC activity (P > 0.05). But it delayed the wound healing (P < 0.01) and inhibited scar hyperplasia (P <0.05).


CONCLUSIONS
PKC might not mediate the signal of IFN-gamma inhibiting the wound healing and scar hyperplasia. But PKC might be related to the wound healing and scar hyperplasia.
Zhang
Xuan-fen
XF
Department of Plastic Surgery, Second Hospital of Lanzhou University, Lanzhou 730030, China.
Guo
Shu-zhong
SZ
Zhang
Lin-xi
LX
Liu
Kai
K
D000818:Animals; D002921:Cicatrix / Q000378:metabolism*; D005260:Female; D007371:Interferon-gamma / Q000494:pharmacology*; D008297:Male; D011493:Protein Kinase C / Q000187:drug effects* / Q000378:metabolism*; D011817:Rabbits; D015398:Signal Transduction; D012867:Skin / Q000293:injuries; D014945:Wound Healing / Q000187:drug effects*
D007371:Interferon-gamma; D011493:Protein Kinase C