title: Novel mitochondria-localizing TEMPO derivative for measurement of cellular oxidative stress in mitochondria.
abstract: Neurodegenerative disorders, such as Alzheimer's and Parkinson's diseases, and apoptosis, are thought to be associated with oxidative stress affecting mitochondria. In this study, we designed and synthesized a fluorescein-tagged TEMPO derivative, compound 1, with triphenylphosphino moiety. Synthesized 1 localized in mitochondria and detected oxidative stress in an endotoxic model of a mouse macrophage-like cell line. Compound 1 is therefore a potentially useful probe for evaluating oxidative stress in mitochondria.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya, Aichi 467-8603, Japan.
authors.forename: Shizuka
authors.initials: S
authors.lastname: Ban
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Nakagawa
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Naoki
authors.initials: N
authors.lastname: Miyata
mesh_terms: D000818:Animals; D002460:Cell Line; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D003497:Cyclic N-Oxides / Q000737:chemistry* / Q000494:pharmacology*; D015195:Drug Design; D004578:Electron Spin Resonance Spectroscopy; D051379:Mice; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism*; D008956:Models, Chemical; D008968:Molecular Conformation; D018384:Oxidative Stress; D013997:Time Factors
chemical_list: D003497:Cyclic N-Oxides; C003959:TEMPO