[Isolation and identification of a phenanthrene-degrading bacterial strain].
Through selective enriched culture, a phenanthrene-degrading bacterial strain was isolated from the oil-contaminated soil in Shenfu irrigation area of Shenyang, Northeast China. The morphological and physiological-biochemical identification, 16S rDNA sequence analysis, and phylogenetic study showed that this strain was belonged to genus Acinetobacter and named as Acinetobacter sp. L2, and closest to Acinetobacter sp. DG880 [AY258108]. It could use phenanthrene as the sole carbon source. After 7 days culture, the degradation rate of phenanthrene was 96.3%. According to the activity of catechol 2,3-dioxygenase, the strain probably had phenanthrene-degrading genes.
Zhu
Rugang
R
Key Laboratory of Agricultural Biotechnology of Liaoning Province, Shenyang Agricultural University, Shenyang 110161, China. zrg_luck@163.com
Zhong
Ming
M
Zhou
Qixing
Q
Liu
Haining
H
Li
Yushuang
Y
D000150:Acinetobacter / Q000201:enzymology / Q000302:isolation & purification* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D050557:Catechol 2,3-Dioxygenase / Q000378:metabolism*; D010616:Phenanthrenes / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017421:Sequence Analysis
D010616:Phenanthrenes; D012336:RNA, Ribosomal, 16S; C031181:phenanthrene; D050557:Catechol 2,3-Dioxygenase