[Study of gene expression of cytokines and apoptotic factors in blood and tissues from patients with surgery infection by the method of real-time polymerase chain reaction].
The expression of cytokines and markers of apoptosis was studied in the whole blood of 10 volunteers by means of reverse transcription method combined with real-time PCR. These factors were also measured in the whole blood, in inflammation nodus tissues and in amputation level nodal tissues taken from 17 patients with local surgical infection. No expression of examined factors in whole blood of volunteers has been observed. However, genes of cytokines and apoptotic markers were expressed in different levels in lymphocytes of whole blood for the case of patients with local surgery infection. This expression was lower in tissues from amputation level and was almost absent in tissues from inflammation nodus, except of the gene, encoding caspase-8. These results suggest that number of pro- and antiinflammating cytokines and apoptotic factors are expressed in peripheral blood and tissue lymphocytes at the formation of System Inflammation Response Syndrom.
Globa
A G
AG
Dikova
O N
ON
Demidova
V S
VS
Karelin
A A
AA
D000671:Amputation, Surgical / Q000009:adverse effects; D017209:Apoptosis / Q000235:genetics; D015415:Biomarkers / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics; D006801:Humans; D007239:Infections / Q000209:etiology / Q000235:genetics / Q000378:metabolism*; D007249:Inflammation / Q000209:etiology / Q000235:genetics / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018746:Systemic Inflammatory Response Syndrome / Q000209:etiology / Q000235:genetics / Q000378:metabolism
D015415:Biomarkers; D016207:Cytokines