title: Detection of low-copy-number genomic DNA sequences in individual bacterial cells by using peptide nucleic acid-assisted rolling-circle amplification and fluorescence in situ hybridization.
abstract: An approach is proposed for in situ detection of short signature DNA sequences present in single copies per bacterial genome. The site is locally opened by peptide nucleic acids, and a circular oligonucleotide is assembled. The amplicon generated by rolling circle amplification is detected by hybridization with fluorescently labeled decorator probes.
authors.affiliation: Center for Advanced Biotechnology and Department of Biomedical Engineering, Boston University, 36 Cummington St., Boston, MA 02215, USA.
authors.forename: Irina
authors.initials: I
authors.lastname: Smolina
authors.forename: Charles
authors.initials: C
authors.lastname: Lee
authors.forename: Maxim
authors.initials: M
authors.lastname: Frank-Kamenetskii
mesh_terms: D001412:Bacillus subtilis / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis* / Q000737:chemistry; D004926:Escherichia coli / Q000302:isolation & purification*; D018628:Gene Dosage; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D020135:Peptide Nucleic Acids / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D020135:Peptide Nucleic Acids