title: Detection and identification by PCR of a highly virulent phylogenetic subgroup among extraintestinal pathogenic Escherichia coli B2 strains.
abstract: Closely related Escherichia coli B2 strains O1:K1, O2:K1, O18:K1, and O45:K1 constitute a major subgroup causing extraintestinal infections. A DNA pathoarray analysis was used to develop a PCR specific for this subgroup that was included in the multiplex phylogenetic-grouping PCR method. Our PCR may serve to identify this virulent subgroup among different ecological niches.
authors.affiliation: Service de Microbiologie, Hôpital Robert Debré, 48 Bd Sérurier, 75395 Paris cedex 19, France. edouard.bingen@rdb.ap-hop-paris.fr.
authors.forename: Philippe
authors.initials: P
authors.lastname: Bidet
authors.forename: Arnaud
authors.initials: A
authors.lastname: Metais
authors.forename: Farah
authors.initials: F
authors.lastname: Mahjoub-Messai
authors.forename: Lionel
authors.initials: L
authors.lastname: Durand
authors.forename: Marie
authors.initials: M
authors.lastname: Dehem
authors.forename: Yannick
authors.initials: Y
authors.lastname: Aujard
authors.forename: Edouard
authors.initials: E
authors.lastname: Bingen
authors.forename: Xavier
authors.initials: X
authors.lastname: Nassif
authors.forename: Stéphane
authors.initials: S
authors.lastname: Bonacorsi
mesh_terms: D001483:Base Sequence; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D021521:Ribotyping; D014774:Virulence