title: Lissoclibadins 4-7, polysulfur aromatic alkaloids from the Indonesian ascidian Lissoclinum cf. badium.
abstract: Four new polysulfur aromatic alkaloids, lissoclibadins 4 (1), 5 (2), 6 (3), and 7 (4), were isolated from the ascidian Lissoclinum cf. badium collected in Indonesia, together with seven known alkaloids, lissoclibadins 1 (5), 2 (6), and 3 (7), lissoclinotoxins E (8) and F (9), 3,4-dimethoxy-6-(2'-N,N-dimethylaminoethyl)-5-(methylthio)benzotrithiane (10), and N,N-dimethyl-5-(methylthio)varacin (11). Compounds 1-11 were isolated from the ascidian collected in March (wet season), while 5-11 have been obtained previously from the organism collected in September (dry season) at the same site. The structures of the new compounds were assigned on the basis of their spectroscopic data. Lissoclibadins 4-7 (1-4) inhibited the colony formation of Chinese hamster V79 cells with EC50 values of 0.71, 0.06, 0.06, and 0.17 microM, respectively. Compounds 1-4 showed also weak antimicrobial activity against Staphylococcus aureus, Escherichia coli, and Saccharomyces cerevisiae.
authors.affiliation: Tohoku Pharmaceutical University, Aoba-ku, Sendai 981-8558, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Nakazawa
authors.forename: Jingzhon
authors.initials: J
authors.lastname: Xu
authors.forename: Teruaki
authors.initials: T
authors.lastname: Nishikawa
authors.forename: Taiko
authors.initials: T
authors.lastname: Oda
authors.forename: Ayako
authors.initials: A
authors.lastname: Fujita
authors.forename: Kazuyo
authors.initials: K
authors.lastname: Ukai
authors.forename: Remy E P
authors.initials: RE
authors.lastname: Mangindaan
authors.forename: Henki
authors.initials: H
authors.lastname: Rotinsulu
authors.forename: Hisayoshi
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Michio
authors.initials: M
authors.lastname: Namikoshi
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000818:Animals; D006224:Cricetinae; D003412:Cricetulus; D004926:Escherichia coli / Q000187:drug effects; D007214:Indonesia; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D013455:Sulfur / Q000737:chemistry / Q000494:pharmacology; D014561:Urochordata / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D013455:Sulfur