[Effects of coriaria lactone on the sodium currents of actuely isolated rat hippocampal neurons].
OBJECTIVE
The present study tried to find out the effects of coriaria lactone (CL) on the sodium currents of the rat hippocampal neurons and their significance.


METHODS
The sodium currents of the acutely isolated Sprague-Dawley rat hippocampal neurons were recorded by employing the patch-clamp technique in the whole-cell voltage-clamp mode. The effects of CL on the peak currents in the neuron membrane were assessed and analyzed.


RESULTS
Both 0.1 mg/mL and 0.2 mg/mL CL increased the maximal peak density of sodium currents significantly (17.32%+/-8.52% vs 37.98%+/-4.91%). The effect of 0.2 mg/mL CL (P < 0.05) was more significant than that of 0.1 mg/mL CL (P > 0.05), when compared with the control (3.16%+/-0.65%).


CONCLUSIONS
CL can increase the amplitudes of the voltage-dependent sodium currents in the acutely isolated hippocampal neurons. This effect may contribute to the enhanced excitability of hippocampal neurons and play a role in the epileptogenetic process of CL.
Zhang
Qin
Q
Department of Neurology, West China Hospital, Sichuan University, Chengdu 610041, China.
Lai
Xiao-hui
XH
Liao
Da-qing
DQ
Liu
Jie
J
Zhou
Dong
D
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004553:Electric Conductivity; D006624:Hippocampus / Q000166:cytology* / Q000187:drug effects* / Q000502:physiology; D066298:In Vitro Techniques; D007783:Lactones / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D051381:Rats; D012964:Sodium / Q000378:metabolism*
D007783:Lactones; C049836:coriaria lactone; D012964:Sodium