Analysis of ethylidene-bridged flavan-3-ols in wine.
A method was developed to determine the amount of ethylidene-bridged flavan-3-ols in wine. The method was based upon the analysis of 2,2'-ethylidenediphloroglucinol (EDP), a product formed after acid-catalyzed cleavage of wine flavan-3-ols in the presence of excess phloroglucinol. In the developed analytical method, the wine was purified and concentrated using C18 solid-phase extraction before the phloroglucinolysis reaction was carried out. This procedure was used to quantify ethylidene-bridged flavan-3-ols in wine and the molar ratio between ethylidene-bridged linkages and native interflavan linkages. The method validation showed 9.2% repeatability. The recovery of the ethylidene-bridged flavanols in wine was 90% for concentrations up to 4.5 mg.L-1 of ethylidene-bridged linkages, and it decreases to 83% above and until the concentration reached 7.6 mg.L-1. Initial results showed that the concentration of ethylidene-bridged flavan-3-ols measured in wines was very low (less than 1.3 mg.L-1) and that they represented less than 1.3% of the total interflavonoid linkages on a molar ratio.
Drinkine
Jessica
J
Faculté d'Oenologie de Bordeaux, Université Victor Segalen Bordeaux 2, UMR 1219 INRA ISVV, 351 Cours de la Libération, 33405 Talence Cedex, France.
Lopes
Paulo
P
Kennedy
James A
JA
Teissedre
Pierre-Louis
PL
Saucier
Cedric
C
D002392:Catechin / Q000032:analysis; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D010696:Phloroglucinol / Q000031:analogs & derivatives* / Q000032:analysis; D015203:Reproducibility of Results; D014920:Wine / Q000032:analysis*
C520047:2,2'-ethylidenediphloroglucinol; D005419:Flavonoids; C404987:flavan-3-ol; D002392:Catechin; D010696:Phloroglucinol