Characterization of polymethoxylated flavones in Fructus aurantii by liquid chromatography with atmospheric pressure chemical ionization combined with tandem mass spectrometry.
Atmospheric pressure chemical ionization mass spectrometry (APCI-MS) was operated in positive mode (PI) to characterize polymethoxylated flavonoids (PMFs) through its specific radical cations by collision-induced dissociation (CID). The fragments of [M + H - n x 15]+ produced by loss of one or more methyl group from the protonated molecule, as well as [M + H - 29]+, [M + H - 31]+, [M + H - 33]+, [M + H - 43]+, [M + H - 46]+, and [M + H - 61]+ fragment ions were detected, which were diagnostic for the polymethoxylated species, and could be adopted to form the multiple MS (MS(n)) "fingerprint" of PMFs. Based on this "fingerprint", 29 PMFs were screened out from extracts of Fructus aurantii, among which two of them were identified as sinensetin and tangeretin. It was proved that the PI was suitable for structural characterization of PMFs by APCI-MS(n).
Zhou
Da-Yong
DY
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China.
Chen
De-Liang
DL
Xu
Qing
Q
Xue
Xing-Ya
XY
Zhang
Fei-Fang
FF
Liang
Xin-Miao
XM
D001274:Atmospheric Pressure; D002853:Chromatography, Liquid / Q000379:methods*; D002957:Citrus / Q000737:chemistry*; D047309:Flavones / Q000032:analysis* / Q000737:chemistry*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D053719:Tandem Mass Spectrometry / Q000379:methods*
D047309:Flavones; D010936:Plant Extracts