[Use of molecular genetic systems for diagnostics of inflammatory diseases of oral mucosa and periodontium].
Structure of bacterial microflora of periodontal pocket using classical cultural and molecular genetic methods (PCR and RT-PCR assays) was studied in patients with chronic generalized severe or intermediate periodontitis in acute phase and in healthy subjects. Structure of viral and fungal microflora using PCR or ELISA assays was studied also. Markers of Gram-negative bacteria (B. forsithus, T. denticola, A. actinomicetemcommitans, P. intermedia and P. gingivalis) were detected with different rates. Rate of detection of pigment-forming P. intermedia and P. gingivalis was significantly higher when PCR assay was used compared with traditional bacteriologic method. Detection rate of Candida albicans DNA in tooth-gingival fluid was virtually not different from one when cultural method was used. PCR assay is recommended as laboratory method for detection of DNA of microorganisms associated with inflammatory diseases of oral mucosa and periodontium.
Tsarev
V N
VN
Nikolaeva
E N
EN
Nosik
A S
AS
D001431:Bacteriological Techniques; D002176:Candida albicans / Q000235:genetics / Q000302:isolation & purification; D002177:Candidiasis / Q000175:diagnosis / Q000382:microbiology*; D002353:Carrier State / Q000175:diagnosis / Q000382:microbiology; D002908:Chronic Disease; D004269:DNA, Bacterial / Q000032:analysis; D004271:DNA, Fungal / Q000032:analysis; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000302:isolation & purification*; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis / Q000382:microbiology*; D006801:Humans; D007249:Inflammation / Q000382:microbiology / Q000473:pathology; D010514:Periodontal Pocket / Q000382:microbiology; D010518:Periodontitis / Q000175:diagnosis / Q000382:microbiology*; D010519:Periodontium / Q000382:microbiology / Q000473:pathology; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial; D004271:DNA, Fungal; D011933:Reagent Kits, Diagnostic