title: Selective desulfurization of cysteine in the presence of Cys(Acm) in polypeptides obtained by native chemical ligation.
abstract: Increased versatility for the synthesis of proteins and peptides by native chemical ligation requires the ability to ligate at positions other than Cys. Here, we report that Raney nickel can be used under standard conditions for the selective desulfurization of Cys in the presence of Cys(Acm). This simple and practical tactic enables the more common Xaa-Ala junctions to be used as ligation sites for the chemical synthesis of Cys-containing peptides and proteins. [reaction: see text].
authors.affiliation: Department of Chemistry, Institute for Biophysical Dynamics, Gordon Center for Integrative Science, The University of Chicago, Chicago Illinois 60637, USA.
authors.forename: Brad L
authors.initials: BL
authors.lastname: Pentelute
authors.forename: Stephen B H
authors.initials: SB
authors.lastname: Kent
mesh_terms: D000595:Amino Acid Sequence; D000682:Amyloid / Q000138:chemical synthesis; D003545:Cysteine / Q000031:analogs & derivatives* / Q000737:chemistry*; D058228:Islet Amyloid Polypeptide; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis*; D021241:Spectrometry, Mass, Electrospray Ionization; D013455:Sulfur / Q000737:chemistry*
chemical_list: D000682:Amyloid; D058228:Islet Amyloid Polypeptide; D010455:Peptides; D013455:Sulfur; D003545:Cysteine