title: Analysis of minimal residual disease by Ig/TCR gene rearrangements: guidelines for interpretation of real-time quantitative PCR data.
abstract: Most modern treatment protocols for acute lymphoblastic leukaemia (ALL) include the analysis of minimal residual disease (MRD). To ensure comparable MRD results between different MRD-polymerase chain reaction (PCR) laboratories, standardization and quality control are essential. The European Study Group on MRD detection in ALL (ESG-MRD-ALL), consisting of 30 MRD-PCR laboratories worldwide, has developed guidelines for the interpretation of real-time quantitative PCR-based MRD data. The application of these guidelines ensures identical interpretation of MRD data between different laboratories of the same MRD-based clinical protocol. Furthermore, the ESG-MRD-ALL guidelines will facilitate the comparison of MRD data obtained in different treatment protocols, including those with new drugs.
authors.affiliation: Department of Immunology, Erasmus MC, Rotterdam, The Netherlands.
authors.forename: V H J
authors.initials: VH
authors.lastname: van der Velden
authors.forename: G
authors.initials: G
authors.lastname: Cazzaniga
authors.forename: A
authors.initials: A
authors.lastname: Schrauder
authors.forename: J
authors.initials: J
authors.lastname: Hancock
authors.forename: P
authors.initials: P
authors.lastname: Bader
authors.forename: E R
authors.initials: ER
authors.lastname: Panzer-Grumayer
authors.forename: T
authors.initials: T
authors.lastname: Flohr
authors.forename: R
authors.initials: R
authors.lastname: Sutton
authors.forename: H
authors.initials: H
authors.lastname: Cave
authors.forename: H O
authors.initials: HO
authors.lastname: Madsen
authors.forename: J M
authors.initials: JM
authors.lastname: Cayuela
authors.forename: J
authors.initials: J
authors.lastname: Trka
authors.forename: C
authors.initials: C
authors.lastname: Eckert
authors.forename: L
authors.initials: L
authors.lastname: Foroni
authors.forename: U
authors.initials: U
authors.lastname: Zur Stadt
authors.forename: K
authors.initials: K
authors.lastname: Beldjord
authors.forename: T
authors.initials: T
authors.lastname: Raff
authors.forename: C E
authors.initials: CE
authors.lastname: van der Schoot
authors.forename: J J M
authors.initials: JJ
authors.lastname: van Dongen
mesh_terms: D004273:DNA, Neoplasm / Q000235:genetics; D015321:Gene Rearrangement; D005803:Genes, Immunoglobulin; D006801:Humans; D018365:Neoplasm, Residual / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000382:microbiology; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm; D011948:Receptors, Antigen, T-Cell