title: Bile salts stimulate recruitment of IpaB to the Shigella flexneri surface, where it colocalizes with IpaD at the tip of the type III secretion needle.
abstract: Shigella flexneri uses its type III secretion apparatus (TTSA) to deliver invasins into human cells. This TTSA possesses an external needle with IpaD at its tip. We now show that deoxycholate promotes the stable recruitment of IpaB to the needle tip without inducing a rapid burst of type III secretion. The maintenance of IpaB at the needle tip requires a stable association of IpaD with the Shigella surface. This is the first demonstration of a translocator protein being stably associated with the TTSA needle.
authors.affiliation: Department of Molecular Biosciences, University of Kansas, 1200 Sunnyside Avenue, Lawrence, KS 66045, USA.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Olive
authors.forename: Roma
authors.initials: R
authors.lastname: Kenjale
authors.forename: Marianela
authors.initials: M
authors.lastname: Espina
authors.forename: David S
authors.initials: DS
authors.lastname: Moore
authors.forename: Wendy L
authors.initials: WL
authors.lastname: Picking
authors.forename: William D
authors.initials: WD
authors.lastname: Picking
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001647:Bile Acids and Salts / Q000494:pharmacology*; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D046529:Microscopy, Electron, Transmission; D009154:Mutation; D012763:Shigella flexneri / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D014774:Virulence
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001647:Bile Acids and Salts; C095770:IpaD protein, Shigella flexneri; C087447:ipaB protein, Shigella