title: Hepatic pre-sinusoidal vessels contract in anaphylactic hypotension in rabbits.
abstract: OBJECTIVE
  The purpose of this study was to determine whether anaphylactic hypotension in rabbits is accompanied by hepatic venoconstriction, and the effects of anaphylaxis on hepatic segmental vascular resistances and liver weight in isolated perfused rabbit livers.
  
  
  METHODS
  The rabbits were sensitized by subcutaneous injection of antigen of 2.5 mg ovalbumin with complete Freund's adjuvant three times at 1 week interval. One week after sensitization, anaphylaxis was induced by an injection of 2.5 mg ovalbumin into the jugular vein of pentobarbital anaesthetized rabbits or the perfusate of rabbit livers perfused via the portal vein at a constant flow. Using the double occlusion technique to estimate the hepatic sinusoidal pressure, pre- (R(pre)) and post-sinusoidal (R(post)) resistances were calculated for the isolated perfused livers.
  
  
  RESULTS
  An antigen injection into the sensitized rabbits caused not only a decrease in systemic arterial pressure from 79 +/- 2 to 40 +/- 4 mmHg, but also an increase in portal venous pressure (P(pv)) from 9.5 +/- 2.2 to 24.1 +/- 3.9 cmH(2)O. Portal hypertension persisted for 8 min after the antigen injection. An injection of antigen into the perfusate caused a marked increase in P(pv) from 5.4 +/- 0.1 to 28.6 +/- 2.4 cmH(2)O at 6 min, but only a slight increase in double occlusion pressure from 2.2 +/- 0.2 to 3.8 +/- 0.2 cmH(2)O, resulting in a selective increase in R(pret) rather than R(post). Concomitant with the hepatic pre-sinusoidal constriction, liver weight loss occurred.
  
  
  CONCLUSIONS
  Anaphylactic hypotension in rabbits is accompanied by hepatic venoconstriction which is characterized by pre-sinusoidal contraction.
authors.affiliation: Department of Physiology, Kanazawa Medical University, Uchinada, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Karasawa
authors.forename: T
authors.initials: T
authors.lastname: Shibamoto
authors.forename: S
authors.initials: S
authors.lastname: Cui
authors.forename: H
authors.initials: H
authors.lastname: Takano
authors.forename: Y
authors.initials: Y
authors.lastname: Kurata
authors.forename: H
authors.initials: H
authors.lastname: Tsuchida
mesh_terms: D000707:Anaphylaxis / Q000276:immunology / Q000503:physiopathology*; D000818:Animals; D000941:Antigens / Q000008:administration & dosage; D001794:Blood Pressure / Q000502:physiology; D003251:Constriction, Pathologic / Q000503:physiopathology; D006503:Hepatic Veins / Q000503:physiopathology; D007022:Hypotension / Q000276:immunology / Q000503:physiopathology*; D007279:Injections, Subcutaneous; D008099:Liver / Q000098:blood supply* / Q000276:immunology; D008102:Liver Circulation / Q000502:physiology*; D008297:Male; D009929:Organ Size / Q000502:physiology; D010477:Perfusion; D011169:Portal Vein / Q000503:physiopathology; D011817:Rabbits; D014655:Vascular Resistance / Q000502:physiology
chemical_list: D000941:Antigens