Influence of Cr(III) and Cr(VI) on the interaction between sparfloxacin and calf thymus DNA.
Cr(III) and Cr(VI) have different binding capacity with sparfloxacin, and have different combination modes with calf thymus DNA. Selecting these two different metal ions, the influence of them on the binding constants between sparfloxacin (SPFX) and calf thymus DNA, as well as the related mechanism has been studied by using absorption and fluorescence spectroscopy. The result shows that Cr(III) has weaker binding capacity to SPFX in the SPFX-Cr(III) binary system, but influences the binding between SPFX and DNA obviously in SPFX-DNA-Cr(III) ternary system. However, although Cr(VI) has a stronger binding capacity to SPFX, it has no effect on the binding between SPFX and DNA. Referring to the different modes of Cr(III) and Cr(VI) binding to DNA, the mechanism of the influence of metal ions on the binding between SPFX and DNA has been proposed. SPFX can directly bind to DNA by chelating DNA base sites. If a metal ion at certain concentration binds mainly to DNA bases, it can decrease the binding constants between SPFX and DNA through competing with SPFX. While if a metal ion at certain concentration mainly binds to phosphate groups of DNA, it can increase the binding constants by building a bridge between SPFX and DNA. If a metal ion at certain concentrations binds neither to bases nor phosphate groups in DNA, it will have no effect on the binding constant between SPFX and DNA. Our result supports Palumbo's conclusion that the binding between SPFX and the phosphata groups is the precondition for the combination between SPFX and DNA, which is stabilized through stacking interactions between the condensed rings of SPFX and DNA bases.
Guo
Dong-Sheng
DS
College of Environment and Resources, Shanxi University, Wucheng Road 580, Taiyuan 030006, China. dongsguo@sxu.edu.cn
Yuan
Xiao-Ying
XY
Wu
Jian-Bing
JB
D000818:Animals; D001667:Binding, Competitive; D002417:Cattle; D002857:Chromium / Q000737:chemistry*; D017608:Chromium Compounds / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D024841:Fluoroquinolones / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D017608:Chromium Compounds; D024841:Fluoroquinolones; D002857:Chromium; D004247:DNA; C427203:calf thymus DNA; C061363:sparfloxacin