title: 3D model of RNA polymerase and bidirectional transcription.
abstract: In the in vitro mitochondrial (mt) transcription initiation system with mt RNA polymerase fraction and mt lysate, the transcription initiation products were shown to be synthesized bidirectionally from the only H-strand-promoter (HSP)/L-strand-promoter region (LSP) of the mitochondrial D-loop genome segment. These transcription products ranged between >100 and >800 bp with the purified mitochondrial RNA polymerase fraction, but were larger (>2030-4000 bp) in size with the mitochondrial lysate in both human and mouse. In this brief report, an in vitro reconstituted mitochondrial transcription system purified by affinity chromatography (heparin-Sepharose) from mouse hypotetraploid letter Ehrlich ascites tumor cell mitochondria was shown to initiate transcription bidirectionally from the mitochondrial D-loop region (HSP/LSP), as evidenced by in vitro generated transcription products. The in vitro generated transcription products were separated by sequencing gel. But this in vitro reconstituted transcription system was not studied beyond the D-loop region. A 3D model of the enzyme RNA polymerase was docked with both ATP and CTP.
authors.affiliation: Department of Biochemistry, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA. ronaldraegon@yahoo.com
authors.forename: Pradip
authors.initials: P
authors.lastname: Bhattacharya
mesh_terms: D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry* / Q000378:metabolism*; D006860:Hydrogen Bonding; D008297:Male; D051379:Mice; D008928:Mitochondria / Q000201:enzymology / Q000235:genetics; D008930:Mitochondria, Liver / Q000201:enzymology / Q000235:genetics; D008958:Models, Molecular; D051381:Rats; D017207:Rats, Sprague-Dawley; D013367:Submitochondrial Particles / Q000201:enzymology / Q000235:genetics; D013698:Templates, Genetic; D013816:Thermodynamics; D014158:Transcription, Genetic
chemical_list: D012321:DNA-Directed RNA Polymerases