title: Effects of gonadotropin-releasing hormone analog (GnRHa) on steroidogenic factor-1 (SF-1) and estrogen receptor beta (ERbeta) gene expression in the black porgy (Acanthopagrus schlegeli).
abstract: We examined effects of GnRHa on expression of steroidogenic factor-1 (SF-1) and estrogen receptor beta (ERbeta) in the pituitary and gonad of protandrous black porgy (Acanthopagrus schlegeli). Fish were intraperitoneally injected with 0.2 microg GnRHa/g fish and then pituitary, gonad and plasma were sampled at 0, 6, 12, 24 and 48 h after injection. In gonad, the mRNA levels of the SF-1 were high at 6 h post injection, and then continuously decreased until 24 h; high expression of ERbeta mRNA levels was only observed at 12 h. In contrast, pituitary SF-1 mRNA levels were very low during the experimental period. GnRHa stimulation caused a significant increase of plasma testosterone (T) and estradiol-17beta (E(2)) after 24 h. We suggest that SF-1 and ERbeta play an important role in the development of gonad and these genes are involved with sex change in fish.
authors.affiliation: Division of Marine Environment and Bioscience, Korea Maritime University, Busan, Korea. choic@hhu.ac.kr
authors.forename: Cheol Young
authors.initials: CY
authors.lastname: Choi
authors.forename: Kwang Wook
authors.initials: KW
authors.lastname: An
authors.forename: Pil Gue
authors.initials: PG
authors.lastname: Jo
authors.forename: Duk-Young
authors.initials: DY
authors.lastname: Kang
authors.forename: Young Jin
authors.initials: YJ
authors.lastname: Chang
mesh_terms: D000818:Animals; D004958:Estradiol / Q000097:blood; D047629:Estrogen Receptor beta / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives* / Q000494:pharmacology*; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D010473:Perciformes / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics* / Q000378:metabolism; D054339:Steroidogenic Factor 1; D013739:Testosterone / Q000097:blood; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D047629:Estrogen Receptor beta; D018398:Homeodomain Proteins; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D054339:Steroidogenic Factor 1; D014157:Transcription Factors; D007987:Gonadotropin-Releasing Hormone; D013739:Testosterone; D004958:Estradiol