title: Multisite recording of extracellular potentials produced by microchannel-confined neurons in-vitro.
abstract: Towards establishing electrical interfaces with patterned in vitro neurons, we have previously described the fabrication of hybrid elastomer-glass devices polymer-on-multielectrode array technology and obtained single-electrode recordings of extracellular potentials from confined neurons (Claverol-Tinturé et al., 2005). Here, we demonstrate the feasibility of spatially localized multisite recordings from individual microchannel-guided neurites extending from microwell-confined somas with good signal-to-noise ratios (20 dB) and spike magnitudes of up to 300 microV. Single-cell current source density (scCSD) analysis of the spatio-temporal patterns of membrane currents along individual processes is illustrated.
authors.affiliation: Department of Electronics, Technical University of Catalonia, Barcelona 08028, Spain. ectmail@eel.upc.edu
authors.forename: E
authors.initials: E
authors.lastname: Claverol-Tinturé
authors.forename: J
authors.initials: J
authors.lastname: Cabestany
authors.forename: X
authors.initials: X
authors.lastname: Rosell
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002478:Cells, Cultured; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008839:Microelectrodes; D009415:Nerve Net / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D012908:Snails / Q000502:physiology*