Surface modification of three-dimensional poly(d,l-lactic acid) scaffolds with baicalin: a histological study.
To improve the biocompatibility of three dimensional (3D) poly(d,l-lactic acid) (PDLLA), surface modification with baicalin was performed via a physical entrapment method in this study. The tissue reactions and bone conductivities of such modified PDLLA scaffolds were histologically assessed by using a rabbit radialis defect model in vivo. The native PDLLA scaffolds were prepared via a thermally induced phase separation technique and were characterized by scanning electron microscopy and fluorescence microscopy. The tissue reactions and bone conductivities of both baicalin-modified PDLLA scaffolds and native PDLLA scaffolds were comparably evaluated with histological assay and histomorphometry at different implantation intervals (2, 4, 8 and 12 weeks). Osteocalcin assay, a method to evaluate the bone formation potential, has shown that the osteocalcin production in the baicalin-modified 3D PDLLA scaffold group was significantly higher (p<0.01 or <0.05) than that in the control. Histological observation and histomorphometry results reflected a higher bone formation potential and better biocompatibility of baicalin-modified PDLLA scaffold when compared with those of the native PDLLA scaffolds.
Cai
Kaiyong
K
College of Bioengineering, Chong Qing University, Chong Qing 400044, China. Kaiyong_cai@cqu.edu.cn
Yao
Kangde
K
Yang
Zhiming
Z
Li
Xiuqiong
X
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000627:therapeutic use*; D005419:Flavonoids / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry* / Q000627:therapeutic use*; D008970:Molecular Weight; D015675:Osteocalcin / Q000032:analysis; D011091:Polyesters; D011108:Polymers / Q000737:chemistry* / Q000627:therapeutic use*; D016062:Porosity; D019736:Prostheses and Implants; D011817:Rabbits; D011884:Radius / Q000293:injuries / Q000473:pathology / Q000648:ultrastructure; D013499:Surface Properties; D013997:Time Factors
D001672:Biocompatible Materials; D005419:Flavonoids; D011091:Polyesters; D011108:Polymers; D015675:Osteocalcin; D019344:Lactic Acid; C038044:baicalin; C033616:poly(lactide)