Poly(vinyl alcohol) functionalized poly(dimethylsiloxane) solid surface for immunoassay.
In this communication, we describe a simple and robust method for the covalent bonding of poly(vinyl alcohol) (PVA) on a silanized poly(dimethylsiloxane) (PDMS) surface. Nonspecific adsorption of proteins via hydrophobic-hydrophobic interactions of the PVA-coated surface is greatly reduced, and biomolecules can be rapidly anchored on the PVA-coated surface with high loading and uniformity. On the basis of a sandwich immunoassay with the anti-rabbit IgG and IgG pair as a model, the detection limit for IgG is down to 1 pg/mL with linearity up to 11 microg the levels often encountered in biological, forensic, and environmental samples.
Yu
Ling
L
School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore.
Li
Chang Ming
CM
Zhou
Qin
Q
Luong
John H T
JH
D000818:Animals; D002417:Cattle; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D007118:Immunoassay; D007074:Immunoglobulin G / Q000032:analysis* / Q000737:chemistry; D018625:Microscopy, Atomic Force; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D011817:Rabbits; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000032:analysis* / Q000737:chemistry
D004129:Dimethylpolysiloxanes; D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine; D011142:Polyvinyl Alcohol