[Determination of the minimum enrichment time for the qualitative detection of Listeria monocytogenes in minced pork meat using multiplex-PCR, microarray and ELISA].
A qualitative detection of Listeria monocytogenes was performed in spiked minced pork meat using ELISA, Multiplex-PCR, Microarray and cultural reference method. Minced pork meat in batches of 10 or 25 g was spiked with 25 cfu Listeria monocytogenes per gram and incubated in selective enrichment solutions. During enrichment samples were collected continiously and a Listeria monocytogenes-ELISA, a Multiplex-PCR with electrophoretical detection (Listeria duplex) and a PCR with detection by Microarray (NUTRI Chip) were performed. The enrichment time after which all sub-samples were positive was defined as minimum enrichment time. With the cultural reference method Listeria monocytogenes was detected in 100 % of the samples after a total analysis time of 5 days. With the ELISA kit used in this study positive results were achieved after enrichment for 24 h. Multiplex-PCR with electrophoretical detection was positive after only 16 h of enrichment. The most sensitive detection method was the microarray. Using this technique, an enrichment time of 10 h was sufficient to get positive results in all samples.
Leidreiter
Melanie
M
Landesamt für Verbraucherschutz und Lebensmittelsicherheit Niedersachsen, Lebensmittelinstitut Oldenburg. Melanie.Leidreiter@laves.niedersachsen.de
Kühne
Michael
M
D000818:Animals; D015169:Colony Count, Microbial; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D006801:Humans; D008089:Listeria monocytogenes / Q000302:isolation & purification*; D008461:Meat Products / Q000382:microbiology*; D046228:Microarray Analysis / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D013552:Swine