title: Epidermal hyperplasia and oral carcinoma in mice overexpressing the transcription factor ATF3 in basal epithelial cells.
abstract: ATF3 is a highly conserved eukaryotic transcription factor that is ubiquitously upregulated transcriptionally during cellular responses to a variety of stresses, in particular DNA damage. However, the role of ATF3 in the DNA damage response is unclear. Transgenic mice that overexpress human ATF3 in basal epithelial cells under the control of the bovine keratin 5 (K5) promoter were constructed and characterized for epidermal alterations. Strong, nuclear expression of the exogenous ATF3 protein was seen in basal cells of the epidermis, hair follicles, and oral mucosa. Hyperplastic changes in the K5-expressing, outer root sheath (ORS) cells of the hair follicle were observed in young mice, resulting in multiple layers of ORS cells in the mature follicle and large aberrantly shaped follicles. Mild hyperplasia of the interfollicular epidermis was also noted, increasing with age. However, no epidermal tumors were identified in BK5.ATF3 mice observed for 16 mo. At 16 mo of age, most transgenic mice exhibited multi-focal areas of hyperplasia and dysplasia in the oral mucosa, with cellular atypia and underlying acute inflammatory changes. Neoplastic lesions were also seen in the oral cavity of BK5.ATF3 mice, including oral squamous cell carcinoma (60% incidence) and basal cell tumors with follicular differentiation (70% incidence), but not in non-transgenic FVB/N littermates. Heterogeneous nuclear expression (or stabilization) of p53 protein was seen in some oral dysplasias, with a patchy distribution primarily in the least differentiated layers of the lesions. This represents the first indication that ATF3 may have oncogenic properties in epithelial cells.
authors.affiliation: Department of Carcinogenesis, The University of Texas, M.D. Anderson Cancer Center, Smithville, Texas 78957, USA.
authors.forename: Aijin
authors.initials: A
authors.lastname: Wang
authors.forename: Stacey
authors.initials: S
authors.lastname: Arantes
authors.forename: Claudio
authors.initials: C
authors.lastname: Conti
authors.forename: Mark
authors.initials: M
authors.lastname: McArthur
authors.forename: C Marcelo
authors.initials: CM
authors.lastname: Aldaz
authors.forename: Michael C
authors.initials: MC
authors.lastname: MacLeod
mesh_terms: D051700:Activating Transcription Factor 3 / Q000502:physiology*; D000818:Animals; D018076:DNA, Complementary / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D005838:Genotype; D006801:Humans; D053555:Keratin-5 / Q000096:biosynthesis; D007633:Keratins / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009062:Mouth Neoplasms / Q000378:metabolism*; D018517:Plant Roots; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: C490449:ATF3 protein, human; D051700:Activating Transcription Factor 3; D018076:DNA, Complementary; D053555:Keratin-5; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53; D007633:Keratins