A new method of defense response analysis using a transient expression system in rice protoplasts.
We established a new plant defense response assay using a transient expression system in rice protoplasts. The assay system sensitively detected defense induction by flagellin, which had previously been assigned to a specific elicitor. Our assay system provides a rapid and efficient way to dissect rice defense mechanisms.
Takai
Ryota
R
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, Japan.
Kaneda
Takashi
T
Isogai
Akira
A
Takayama
Seiji
S
Che
Fang-Sik
FS
D000818:Animals; D002478:Cells, Cultured; D005408:Flagellin / Q000235:genetics / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D017930:Genes, Reporter / Q000235:genetics; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D012275:Oryza / Q000166:cytology / Q000502:physiology*; D010935:Plant Diseases; D011523:Protoplasts / Q000502:physiology*; D048790:Renilla / Q000737:chemistry
D005408:Flagellin; D008156:Luciferases