Electrospun PHBV/collagen composite nanofibrous scaffolds for tissue engineering.
Electrospinning has recently emerged as a leading technique for the formation of nanofibrous structures made of synthetic and natural extracellular matrix components. In this study, nanofibrous scaffolds were obtained by electrospinning a combination of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and type-I collagen in 1,1,1,3,3,3-hexafluoro-2-isopropanol (HIFP). The resulting fibers ranged from 300 to 600 nm in diameter. Their surfaces were characterized by attenuated total reflection Fourier transform infrared spectroscopy, electron spectroscopy for chemical analysis and atomic force microscopy. The PHBV and collagen components of the PHBV/collagen nanofibrous scaffold were biodegraded by PHB depolymerase and a type-I collagenase aqueous solution, respectively. The cell culture experiments indicated that the PHBV/collagen nanofibrous scaffold accelerated the adhesion and growth of NIH3T3 cells more effectively than the PHBV nanofibrous scaffold, thus making the former a good scaffold for tissue engineering.
Meng
Wan
W
Department of Polymer Science, Kyungpook National University, Daegu 702-701, South Korea.
Kim
Se-Yong
SY
Yuan
Jiang
J
Kim
Jung Chul
JC
Kwon
Oh Hyeong
OH
Kawazoe
Naoki
N
Chen
Guoping
G
Ito
Yoshihiro
Y
Kang
Inn-Kyu
IK
D000818:Animals; D002448:Cell Adhesion; D049109:Cell Proliferation; D024042:Collagen Type I / Q000737:chemistry*; D017364:Collagenases / Q000378:metabolism; D051379:Mice; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D041681:NIH 3T3 Cells; D049329:Nanostructures; D011091:Polyesters / Q000737:chemistry*; D020005:Propanols; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D000094122:Polyhydroxybutyrates; D006845:Hydrocarbons, Fluorinated
D024042:Collagen Type I; D017364:Collagenases; D011091:Polyesters; D020005:Propanols; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C001337:hexafluoroisopropanol; D006845:Hydrocarbons, Fluorinated; C417249:collagenase 1