title: [Genotype of TEM- and SHV-type beta-lactamase producing Klebsiella pneumoniae in Guangzhou area].
abstract: OBJECTIVE
  To determine the prevalence and genotypes of TEM- and SHV-type beta-lactamase-producing Klebsiella pneumoniae in Guangzhou area.
  
  
  METHODS
  Genotype determination of TEM- and SHV-type beta-lactamases were performed by PCR amplification followed by denaturing high-performance liquid chromatography (DHPLC) and DNA sequencing among 57 strain of klebsiella pneumonia obtained from the hospitals in Guangzhou.
  
  
  RESULTS
  The genes bla(TEM) and bla(SHV) were amplified from all strains. TEM-116 type ESBLs were found in 56.14% (32/57) of the isolates. SHV-12 (n = 19), -11 (n = 11), -2a (n = 6), -28 (n = 4), -26 (n = 1), -33 (n = 1) and five novel SHV-type beta-lactamases were identified among the SHV-type beta-lactamases. These novel enzymes were designated from SHV-64, -65, -66, -67 and SHV-69, which derived from SHV-1 beta-lactamase or SHV-12 ESBL. A total of 94.74% of the isolates (54/57) produced both TEM- and SHV-type beta-lactamases simultaneously, in which 13.0% were TEM-116 and SHV-12 (n = 12).
  
  
  CONCLUSIONS
  TEM-116 and SHV-12 are the predominant TEM- and SHV-type genotypes of Klebsiella pneumoniae producing beta-lactamases in Guangzhou. This area is confronted with the prevalence of the phenotypes of Klebsiella pneumoniae that simultaneously produce TEM- and SHV-type beta-lactamases.
authors.affiliation: First Municipal People's Hospital Affiliated to Guangzhou Medical College, Guangzhou 510180, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zuo
authors.forename: Zhao-Hui
authors.initials: ZH
authors.lastname: Liu
authors.forename: Han-Ping
authors.initials: HP
authors.lastname: Wang
authors.forename: Yin-Mei
authors.initials: YM
authors.lastname: Yang
authors.forename: Jin-Long
authors.initials: JL
authors.lastname: Chen
authors.forename: Hui-Fen
authors.initials: HF
authors.lastname: Ye
mesh_terms: D002681:China / Q000453:epidemiology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology; D005787:Gene Frequency; D005838:Genotype; D007710:Klebsiella Infections / Q000453:epidemiology / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D015995:Prevalence; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D001618:beta-Lactamases