title: [Study on association of interleukin-1 receptor antagonist(RA) gene + 2018 locus mutation with silicosis].
abstract: OBJECTIVE
  To approach whether + 2018 locus (T-- C) mutation within the interleukin- receptor antagonist (IL-1RA) gene promoter region are associated with susceptibility to silicosis in Southwest Chinese Han population.
  
  
  METHODS
  75 cases of silicosis and 137 silica-exposed workers(being employed) were selected as subjects.5ml peripheral vein blood was drawn from each subject. By real-time quantitative polymerase chain reaction and sequencing techniques, IL-1RA gene mutation of all subjects were analyzed.
  
  
  RESULTS
  The frequency of IL-1RA + 2018C among the cases with silicosis was higher than that among the silica-exposed workers (controls) (28.0% versus 15.0% , Fisher' s exact P < 0.01 ). The frequency of allelic C was higher in cases with phase I silicosis than in controls(31.8% versus 15.0%, Fisher's exact P < 0.01 ). The frequency of allelic C was higher in cases with phase I and Ill silicosis than in controls, but which statistic difference being not significant(22.6% versus 15.0%, Fisher's exact P < 0.01). After adjusted for confounding factors, the frequency of IL-1RA + 2018C among silicoses and its subgroups was still higher than among controls, which statistic difference being significant (Fisher' s exact P < 0.01).
  
  
  CONCLUSIONS
  IL-1RA gene polymorphisms played an important role in making the Han population of Southwest China susceptible to silicosis. IL-1RA might be took as a gene to be selected for studying the susceptibility of silicosis.
authors.affiliation: Shandong Center for Disease Control and Prevention, Ji' nan 250014, China.
authors.forename: De-jun
authors.initials: DJ
authors.lastname: Wang
authors.forename: Yue-lin
authors.initials: YL
authors.lastname: Yang
authors.forename: Qing-jie
authors.initials: QJ
authors.lastname: Xia
authors.forename: Ya-jia
authors.initials: YJ
authors.lastname: Lan
authors.forename: Mian-zhen
authors.initials: MZ
authors.lastname: Wang
authors.forename: Ji-ping
authors.initials: JP
authors.lastname: Han
authors.forename: Yu-lin
authors.initials: YL
authors.lastname: Yang
authors.forename: Fan-ling
authors.initials: FL
authors.lastname: Kong
mesh_terms: D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction; D012829:Silicosis / Q000235:genetics*
chemical_list: D053590:Interleukin 1 Receptor Antagonist Protein