Recombinant production and properties of binding of the full set of mouse secreted phospholipases A2 to the mouse M-type receptor.
To date, 12 secreted phospholipases A2 (sPLA2s) have been identified in the mouse species and divided into three structural collections (I/II/V/X, III, and XII). On the basis of their different molecular properties and tissue distributions, each sPLA2 is likely to exert distinct functions by acting as an enzyme or ligand for specific soluble proteins or receptors, among which the M-type receptor is the best-characterized target. Here, we present the properties of binding of the full set of mouse sPLA2s to the mouse M-type receptor. All enzymes have been produced in Escherichia coli or insect cells, and their properties of binding to the cloned and native M-type receptor have been determined. sPLA2s IB, IIA, IIE, IIF, and X are high-affinity ligands (K0.5 = 0.3-3 nM); sPLA2s IIC and V are low-affinity ligands (K0.5 = 30-75 nM), and sPLA2s IID, III, XIIA, and XIIB bind only very weakly or do not bind to the M-type receptor (K0.5 > 100 nM). Three exogenous parvoviral group XIII PLA2s and two fungal group XIV sPLA2s do not bind to the receptor. Together, these results indicate that the mouse M-type receptor is selective for only a subset of mouse sPLA2s from the group I/II/V/X structural collection. Binding of mouse sPLA2s to a recombinant soluble mouse M-type receptor leads in all cases to inhibition of enzymatic activity, and the extent of deglycosylation of the receptor decreases yet does not abolish sPLA2 binding. The physiological meaning of binding of sPLA2 to the M-type receptor is discussed on the basis of our current knowledge of sPLA2 functions.
Rouault
Morgane
M
Institut de Pharmacologie Moléculaire et Cellulaire, CNRS-UMR 6097, 660 route des Lucioles, Sophia Antipolis, 06560 Valbonne, France.
Le Calvez
Catherine
C
Boilard
Eric
E
Surrel
Fanny
F
Singer
Alan
A
Ghomashchi
Farideh
F
Bezzine
Sofiane
S
Scarzello
Sabine
S
Bollinger
James
J
Gelb
Michael H
MH
Lambeau
Gérard
G
D000818:Animals; D003001:Cloning, Molecular; D004330:Drosophila / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D051379:Mice; D010741:Phospholipases A / Q000096:biosynthesis* / Q000378:metabolism; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000502:physiology*; D054507:Receptors, Phospholipase A2; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D018411:Spodoptera / Q000378:metabolism
C516357:Pla2r1 protein, mouse; D011956:Receptors, Cell Surface; D054507:Receptors, Phospholipase A2; D011994:Recombinant Proteins; D010741:Phospholipases A