Genetic variation of Bmy1 alleles in barley (Hordeum vulgare L.) investigated by CAPS analysis.
The enzyme beta-amylase is one of the most important hydrolytic enzymes in the grain of malting barley and is encoded by the gene Bmy1. To learn more about its structure and function, a total of 657 barley accessions including 541 Hordeum vulgare ssp. vulgare (HV), and 116 H. vulgare ssp. spontaneum (HS) were selected for the cleaved amplified polymorphic sequence (CAPS) analysis. These materials, covering all the 16 kinds of beta-amylase phenotypes screened from more than 8,500 accessions of the world barley germplasm, were classified into 13 CAPS types in the present study. A combined assay of phenotypes and CAPS types revealed extensive genetic variation at the Bmy1 locus, and in total 23 Bmy1 allele types were identified. The newly identified alleles (A-I-11, A-II-6, A-II-7, A-II-10, B-I-3, B-I-12 and B-I-13) provided us with a novel resource for barley breeding and Bmy1 study. In HV barley, six out of seven major allele types (C-II-1, B-II-2, B-Ia-3, A-II-5, A-II-6, and A-II-7) were shared with HS barley; the B-I-8 allele, which was predominant in north European cultivated barley, was found to be unique. Remarkably, very low Bmy1 genetic variation was detected in Tibetan barleys, which puts the validity of the hypothesis that Tibet is one of the original centers of cultivated barley into question.
Zhang
Wen Sheng
WS
The State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, People's Republic of China. wszhang@ms.sjziam.ac.cn
Li
Xia
X
Liu
Jian Bing
JB
D000483:Alleles; D004262:DNA Restriction Enzymes / Q000378:metabolism; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D004795:Enzyme Stability; D017343:Genes, Plant; D014644:Genetic Variation; D005843:Geography; D001467:Hordeum / Q000145:classification / Q000235:genetics*; D006358:Hot Temperature; D007525:Isoelectric Focusing; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010940:Plant Proteins / Q000032:analysis / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013698:Templates, Genetic; D001614:beta-Amylase / Q000032:analysis* / Q000235:genetics
D018744:DNA, Plant; D010940:Plant Proteins; D004262:DNA Restriction Enzymes; D001614:beta-Amylase