[The influence of tobacco smoke on plasma atherogenic compounds levels in active smokers (preliminary report)].
The aim of the study was to evaluate the influence of tobacco smoke on plasma concentration of atherogenic compounds, namely total homocysteine (tHcy), symmetric dimethylarginine (SDMA) and asymmetric dimethylarginine (ADMA). The study population consisted of 104 healthy males between the age of 34 and 45 years. Survey questionnaire and determination of plasma cotinine concentration were used to divide the group into smokers (62 males) and non-smokers (42 males). The arbitrary threshold value of plasma cotinine concentration was set to 15 ng/ml. High performance liquid chromatography (HPLC) was used to estimate the plasma concentration of tHcy, ADMA, SDMA and cotinine. Significantly higher (by 38.5%) plasma concentration of tHcy in the group of active smokers in comparison with non-smokers was observed. The concentration of ADMA and SDMA was found out to be higher by 12.8% and 13.5% respectively, however those differences were highly non-significant. Positive significant correlation between concentration of tHcy and cotinine was determined, in the smoking group as well as in the entire study population (r = 0.303, P = 0.017; r 0.257, P = 0.009 respectively). There was no evidence, however, of correlation between the concentration of both ADMA and SDMA and cotinine. Correlation between the concentrations of atherogenic parameters showed weak but significant correlations for tHcy versus ADMA and tHcy versus SDMA and high for the ADMA versus SDMA correlations (r = 0.671 in the smoking group and r = 0.672 in the entire population). The results obtained indicate that tobacco smoke probably has a weak influence on smokers' plasma ADMA and SDMA concentration, while significant rising the concentration of homocysteine, an independent risk factor of atherosclerosis.
Szołtysek-Bołdys
Izabela
I
Zakład Chemii Ogólnej i Nieorganicznej, Katedry Chemii Ogólnej i Analitycznej, Slaskiej Akademii Medycznej w Sosnowcu.
Sobczak
Andrzej
A
Król
Dorota
D
D000328:Adult; D001120:Arginine / Q000031:analogs & derivatives* / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D003367:Cotinine / Q000097:blood; D006711:Homocystine / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012907:Smoking / Q000097:blood*
C487735:dimethylarginine; D006711:Homocystine; C018524:N,N-dimethylarginine; D001120:Arginine; D003367:Cotinine