title: Development of reagents for differential protein quantitation by subtractive parent (precursor) ion scanning.
abstract: We present a generic approach for quantitative differential proteomics that reduces data complexity in proteome analysis by automated selection of peptides for MS/MS analysis according to their isotope-labeling ratio. Isotopic reagents were developed that give products which fragment easily to generate a unique pair of signature ions. Using the ion-pair ratio, we show that it is possible to select only BSA peptides (with a 3:1 light heavy isotope ratio) for MS/MS when spiked in a whole yeast extract using Parent (precursor) Ion Quantitation Scanning (PIQS) for MS/MS.
authors.affiliation: Department of Protein Technology, Lund University, Sweden.
authors.forename: Asa
authors.initials: A
authors.lastname: Wåhlander
authors.forename: Giorgio
authors.initials: G
authors.lastname: Arrigoni
authors.forename: Kristofer
authors.initials: K
authors.lastname: Wårell
authors.forename: Fredrik
authors.initials: F
authors.lastname: Levander
authors.forename: Ronnie
authors.initials: R
authors.lastname: Palmgren
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Maloisel
authors.forename: Philippe
authors.initials: P
authors.lastname: Busson
authors.forename: Peter
authors.initials: P
authors.lastname: James
mesh_terms: D000818:Animals; D007202:Indicators and Reagents; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000302:isolation & purification; D053719:Tandem Mass Spectrometry / Q000379:methods*; D015003:Yeasts
chemical_list: D007202:Indicators and Reagents; D011506:Proteins; D012710:Serum Albumin, Bovine