title: Template matching as a tool for annotation of tomograms of stained biological structures.
abstract: In recent years, electron tomography has improved our three-dimensional (3D) insight in the structural architecture of cells and organelles. For studies that involve the 3D imaging of stained sections, manual annotation of tomographic data has been an important method to help understand the overall 3D morphology of cellular compartments. Here, we postulate that template matching can provide a tool for more objective annotation and contouring of cellular structures. Also, this technique can extract information hitherto unharvested in tomographic studies. To evaluate the performance of template matching on tomograms of stained sections, we generated several templates representing a piece of microtubule or patches of membranes of different staining-thicknesses. These templates were matched to tomograms of stained electron microscopy sections. Both microtubules and ER-Golgi membranes could be detected using this method. By matching cuboids of different thicknesses, we were able to distinguish between coated and non-coated endosomal membrane-domains. Finally, heterogeneity in staining-thickness of endosomes could be observed. Template matching can be a useful addition to existing annotation-methods, and provide additional insights in cellular architecture.
authors.affiliation: Cellular Architecture and Dynamics, Utrecht University, The Netherlands. M.N.Lebbink@bio.uu.nl
authors.forename: Misjaël N
authors.initials: MN
authors.lastname: Lebbink
authors.forename: Willie J C
authors.initials: WJ
authors.lastname: Geerts
authors.forename: Theo P
authors.initials: TP
authors.lastname: van der Krift
authors.forename: Maurice
authors.initials: M
authors.lastname: Bouwhuis
authors.forename: Louis O
authors.initials: LO
authors.lastname: Hertzberger
authors.forename: Arie J
authors.initials: AJ
authors.lastname: Verkleij
authors.forename: Abraham J
authors.initials: AJ
authors.lastname: Koster
mesh_terms: D002462:Cell Membrane / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000648:ultrastructure; D006801:Humans; D021621:Imaging, Three-Dimensional; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D008870:Microtubules / Q000648:ultrastructure; D013194:Staining and Labeling