[Assessment of occurrence frequency of cotinine and trans-3'-hydroxycotinine in chosen body fluid samples].
Kind of the main nicotine metabolites, occurrence frequency of trans-3'-hydroxycotinine (HK) and cotinine (K) in mother and foetus serum, amniotic fluid and urine of chosen group of children (10 - 12 years old) determined by planar chromatography with densitometry as well as ratio of (HK)/(K) has been compared. The obtained data allowed to state, that the main nicotine metabolite concentrations (HK and K) were higher in foetus serum in comparison to mother serum. In the case of occurrence both of them ratio of (HK)/(K) was close to 1 in 20% of examined body fluid samples, but it was above this value in significant majority cases. It can be a prove that trans-3'-hydroxycotinine was dominating metabolite of nicotine in these samples and statistically significant more often occur in foetus serum, in the case when cotinine was determined in mother serum.
Tyrpień
Krystyna
K
Katedra i Zakład Chemii, Slaska Akademia Medyczna w Katowicach. ktyrpien@wp.pl
Wielkoszyński
Tomasz
T
Kowalska
Małgorzata
M
Mańka
Grzegorz
G
Dobosz
Cezary
C
D000653:Amniotic Fluid / Q000737:chemistry; D001826:Body Fluids / Q000737:chemistry*; D002648:Child; D002855:Chromatography, Thin Layer; D003367:Cotinine / Q000031:analogs & derivatives* / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D009538:Nicotine / Q000378:metabolism*; D011247:Pregnancy; D012907:Smoking / Q000378:metabolism*; D014028:Tobacco Smoke Pollution / Q000032:analysis*
D014028:Tobacco Smoke Pollution; C001381:hydroxycotinine; D009538:Nicotine; D003367:Cotinine