Origin and characteristics of the zooplankton phosphatase activity in a coastal ecosystem of the Mediterranean sea (Toulon Bay).
In Toulon Bay (France), very high phosphatase activities have been found in the zooplankton fraction>90 microm. This work was intended to specify their origin. For that purpose, larvae, juvenile and adult Crustacea (Copepods: Calanoids, Cyclopoids, Branchiopods: Cladocera, and Cirripeds) were isolated. Their activities were measured using paranitrophenyl phosphate dissolved in sea water in order to calculate Km (the enzyme half saturation concentration) and Vmax (the reaction rate when the enzyme is saturated with substrate). Vmax were referred to protein contents of the isolated organisms to calculate specific activities. For all zooplankton groups high and low affinity phosphatase activities were found. The low affinity enzyme was responsible for at least 70% of the total phosphatase activity. Its specific activity was higher for larvae than for copepodites and adults. In Cirriped nauplii this activity was particularly high with values which were several hundred times higher than that in other Crustacea. These enzymes had optimum pH close to 8.4, magnesium requirement and were competitively inhibited by orthophosphate. Experiments with intact and lysed Cirriped nauplii confirmed that living organisms had only a weak external activity and showed that most of the activity of these larvae was primarily intracellular.
Lespilette
Magali
M
PROTEE-EBMA EA. 3819, Département de Génie Biologique, Université du Sud Toulon-Var (USTV), BP20132, F-83957 La Garde Cedex, France. boge@univ-tln.fr
Bogé
Gérard
G
Richard
Simone
S
Jamet
Jean Louis
JL
D000818:Animals; D003445:Crustacea / Q000201:enzymology*; D005602:France; D006863:Hydrogen-Ion Concentration; D007814:Larva / Q000201:enzymology; D008522:Mediterranean Sea; D010710:Phosphates / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D011506:Proteins / Q000032:analysis*; D013045:Species Specificity; D018709:Statistics, Nonparametric; D015048:Zooplankton / Q000201:enzymology*
D010710:Phosphates; D011506:Proteins; D010744:Phosphoric Monoester Hydrolases