title: Various applications of immobilized glucose oxidase and polyphenol oxidase in a conducting polymer matrix.
abstract: In this study, glucose oxidase and polyphenol oxidase were immobilized in conducting polymer matrices; polypyrrole and poly(N-(4-(3-thienyl methylene)-oxycarbonyl phenyl) maleimide-co-pyrrole) via electrochemical method. Fourier transform infrared and scanning electron microscope were employed to characterize the copolymer of (N-(4-(3-thienyl methylene)-oxycarbonyl phenyl) maleimide) with pyrrole. Kinetic parameters, maximum reaction rate and Michealis-Menten constant, were determined. Effects of temperature and pH were examined for immobilized enzymes. Also, storage and operational stabilities of enzyme electrodes were investigated. Glucose and polyphenol oxidase enzyme electrodes were used for determination of the glucose amount in orange juices and human serum and phenolic amount in red wines, respectively.
authors.affiliation: Department of Chemistry, Middle East Technical University, 06531 Ankara, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Cil
authors.forename: A E
authors.initials: AE
authors.lastname: Böyükbayram
authors.forename: S
authors.initials: S
authors.lastname: Kiralp
authors.forename: L
authors.initials: L
authors.lastname: Toppare
authors.forename: Y
authors.initials: Y
authors.lastname: Yağci
mesh_terms: D001628:Beverages; D001786:Blood Glucose / Q000032:analysis; D004156:Catechol Oxidase / Q000378:metabolism; D032084:Citrus sinensis / Q000737:chemistry; D004563:Electrochemistry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism; D005947:Glucose / Q000032:analysis; D005949:Glucose Oxidase / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D046911:Macromolecular Substances / Q000138:chemical synthesis / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D010636:Phenols / Q000032:analysis; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry; D011758:Pyrroles / Q000138:chemical synthesis / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014920:Wine / Q000032:analysis
chemical_list: D001786:Blood Glucose; D004800:Enzymes, Immobilized; D046911:Macromolecular Substances; D010636:Phenols; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D005949:Glucose Oxidase; D004156:Catechol Oxidase; D005947:Glucose