title: [Effects of Notch-1 signaling in bone marrow mesenchymal stem cells differentiating into neurons: experiment in vitro].
abstract: OBJECTIVE
  To investigate the role of Notch-1 signaling in bone marrow mesenchymal stem cells (MSCs) differentiating into neurons.
  
  
  METHODS
  Mice Notch-1 small hairpin RNA (mNotch-1 shRNA) was constructed and transfected into the MSCs obtained from the tibiae of BALB/c mice. MSCs transfected with glyceraldehyde-3-phosphate hydoxygenase (GADPH) shRNA and untransfected MSCs were used as controls. The cell survival rate was detected by ELISA. The MSCs of different groups were cultured in Neurobasal-A medium so as to be induced to differentiate into neurons. Apoptosis of the MSCs was detected by TUNEL.
  
  
  RESULTS
  After induction of 6 days the MSCs transfected with mNotch-1 shRNA displayed typical neuronal morphology and high expression of neuron-specific markers: nestin, neuron-specific enolase (NSE), neurofilament 200 (NF 200), and Notch-1 protein, however, gilal fibrillary acidic protein (GFAP), the glia-specific marker, was not detected. The percentage of apoptotic cells in the MSCs transfected with mNotch-1 shRNA was 13.3% +/- 2.3%, significantly higher than those of the MSCs transfected with mGAPH shRNA and untransfected MSCs (4.7% +/- 0.5% and 4.5% +/- 0.4%, both P < 0.01).
  
  
  CONCLUSIONS
  Block of the Notch signal pathway may increase the differentiation of MSCs into neurons.
authors.affiliation: Department of Neurology, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Yan-Jie
authors.initials: YJ
authors.lastname: Jia
authors.forename: Ji-Ping
authors.initials: JP
authors.lastname: Sun
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Zhu
authors.forename: Ya-Jun
authors.initials: YJ
authors.lastname: Lian
authors.forename: Hai-Zhen
authors.initials: HZ
authors.lastname: Wang
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.forename: Bo-Ai
authors.initials: BA
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D002470:Cell Survival / Q000235:genetics / Q000502:physiology; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016900:Neurofilament Proteins / Q000032:analysis; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D051881:Receptor, Notch1 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
chemical_list: D005904:Glial Fibrillary Acidic Protein; D016900:Neurofilament Proteins; D034741:RNA, Small Interfering; D051881:Receptor, Notch1; C051774:neurofilament protein H