Fluorescence analysis with quantum dot probes for hepatoma under one- and two-photon excitation.
A new class of fluorescent probe produced by conjugating semiconductor quantum dots (QDs) with protein molecule is proposed as an alternative to conventional organic labels. However the fluorescence characteristics of the QD bioconjugates are not clear while they are excitied with one- or two-photon laser pulse. We synthesized specific immunofluorescent probes by linking QDs to alpha fetoprotein (AFP) antibody for specific binding alpha-fetoprotein -an important marker for hepatocellular carcinoma cell lines, and archived specific fluorescence detection with the QDs-Anti-AFP in nude mice. Then, we have analyzed the fluorescence characteristics of QDs-Anti-AFP and original QDs both under one- and two-photon excitations. The results demonstrated that QDs-Anti-AFP's fluorescent spectral and lifetime haven't varied much from that of original QDs. Moreover, QDs-Anti-AFP have exhibited higher fluorescence efficiency than QDs under two-photon examination.
Yu
Xuefeng
X
Key Laboratory of Acoustic and Photonic Materials and Devices of Ministry of Education & Department of Physics, Wuhan University, Wuhan 430072, China.
Chen
Liangdong
L
Deng
Yuliang
Y
Li
Kaiyang
K
Wang
Ququan
Q
Li
Yan
Y
Xiao
Si
S
Zhou
Li
L
Luo
Xuan
X
Liu
Jia
J
Pang
Daiwen
D
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis*; D005453:Fluorescence; D005455:Fluorescent Antibody Technique / Q000379:methods*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D008113:Liver Neoplasms / Q000175:diagnosis*; D051379:Mice; D008819:Mice, Nude; D017785:Photons; D045663:Quantum Dots; D000509:alpha-Fetoproteins / Q000032:analysis* / Q000138:chemical synthesis / Q000737:chemistry*
D005456:Fluorescent Dyes; D000509:alpha-Fetoproteins