Extracorporeal bioartificial liver using the radial-flow bioreactor in treatment of fatal experimental hepatic encephalopathy.
An extracorporeal bioartificial liver (BAL) that could prevent death from hepatic encephalopathy in acute hepatic insufficiency was aimed to develop. A functional human hepatocellular carcinoma cell line (FLC-4) was cultured in a radial-flow bioreactor. The function of the BAL was tested in mini-pigs with acute hepatic failure induced by alpha-amanitin and lipopolysaccharide. When the BAL system was connected with cultured FLC-4 to three pigs with hepatic dysfunction, all demonstrated electroencephalographic improvement and survived. Relatively low plasma concentrations of S-100 beta protein, as a marker of astrocytic damage, from pigs with hepatic failure during BAL therapy were noted. BAL therapy can prevent irreversible brain damage from hepatic encephalopathy in experimental acute hepatic failure.
Kanai
Hideki
H
Department of Surgery, The Jikei University School of Medicine, Tokyo, Japan.
Marushima
Hideki
H
Kimura
Naofumi
N
Iwaki
Takamasa
T
Saito
Masaya
M
Maehashi
Haruka
H
Shimizu
Keiko
K
Muto
Makiko
M
Masaki
Takahiro
T
Ohkawa
Kiyoshi
K
Yokoyama
Keitaro
K
Nakayama
Masaaki
M
Harada
Tohru
T
Hano
Hiroshi
H
Hataba
Yoshiaki
Y
Fukuda
Takahiro
T
Nakamura
Masahiko
M
Totsuka
Naoto
N
Ishikawa
Shutaro
S
Unemura
Yasuki
Y
Ishii
Yuji
Y
Yanaga
Katsuhiko
K
Matsuura
Tomokazu
T
D000546:Amanitins; D000818:Animals; D019149:Bioreactors; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D006501:Hepatic Encephalopathy / Q000097:blood / Q000139:chemically induced / Q000628:therapy*; D008070:Lipopolysaccharides; D019164:Liver, Artificial; D008297:Male; D009414:Nerve Growth Factors / Q000097:blood; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000097:blood; D013552:Swine; D013556:Swine, Miniature
D000546:Amanitins; D008070:Lipopolysaccharides; D009414:Nerve Growth Factors; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins