Bacterial outer membrane protein analysis by electrophoresis and microchip technology.
Outer membrane proteins are indispensable components of bacterial cells and participate in several relevant functions of the microorganisms. Changes in the outer membrane protein composition might alter antibiotic sensitivity and pathogenicity. Furthermore, the effects of various factors on outer membrane protein expression, such as antibiotic treatment, mutation, changes in the environment, lipopolysaccharide modification and biofilm formation, have been analyzed. Traditionally, the outer membrane protein profile determination was performed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Converting this technique to capillary electrophoresis format resulted in faster separation, lower sample consumption and automation. Coupling capillary electrophoresis with mass spectrometry enabled the fast identification of bacterial proteins, while immediate quantitative analysis permitted the determination of up- and downregulation of certain outer membrane proteins. Adapting capillary electrophoresis to microchip format ensured a further ten- to 100-fold decrease in separation time. Application of different separation techniques combined with various sensitive detector systems has ensured further opportunities in the field of high-throughput bacterial protein analysis. This review provides an overview using selected examples of outer membrane proteins and the development and application of the electrophoretic and microchip technologies for the analysis of these proteins.
Kustos
Ildikó
I
University of Pécs, Department of Medical Microbiology & Immunology, Faculty of Medicine, Pécs, Hungary. ildiko.kustos@aok.pte.hu
Kocsis
Béla
B
Kilár
Ferenc
F
D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D004586:Electrophoresis; D046208:Microchip Analytical Procedures; D040901:Proteomics / Q000379:methods*
D001425:Bacterial Outer Membrane Proteins