Multiplex PCR assay for detection of Vibrio vulnificus biotype 2 and simultaneous discrimination of serovar E strains.
In the present work we develop a multiplex PCR assay for the detection and identification of the fish pathogen Vibrio vulnificus biotype 2 with discriminating potential for zoonotic strains (serovar E). The PCR assay allowed the identification of two new biotype 2 serovar E human isolates from culture collections. Finally, the multiplex was successfully applied to both diagnosis and carrier detection in field samples.
Sanjuán
Eva
E
Departamento Microbiología y Ecología, Universidad de Valencia, Avenida Dr. Moliner 50, 46100 Burjassot, Valencia, Spain.
Amaro
Carmen
C
D000818:Animals; D001431:Bacteriological Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D014735:Vibrio Infections / Q000382:microbiology* / Q000662:veterinary*; D041261:Vibrio vulnificus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
D004269:DNA, Bacterial