Large-scale analysis of chlorophyll fluorescence kinetics in Synechocystis sp. PCC 6803: identification of the factors involved in the modulation of photosystem stoichiometry.
Since chlorophyll fluorescence reflects the redox state of photosynthetic electron transport chain, monitoring of chlorophyll fluorescence has been successfully applied for the screening of photosynthesis-related genes. Here we report that the mutants having a defect in the regulation of photosystem stoichiometry could be identified through the simple comparison of the induction kinetics of chlorophyll fluorescence. We made a library containing 500 mutants in the cyanobacterium Synechocystis sp. PCC 6803 with transposon-mediated gene disruption, and the mutants were used for the measurement of chlorophyll fluorescence kinetics for 45 s. We picked up two genes, pmgA and sll1961, which are involved in the modulation of photosystem stoichiometry. The disruptants of the two genes share common characteristics in their fluorescence kinetics, and we searched for mutants that showed such characteristics. Out of six mutants identified so far, five showed a different photosystem stoichiometry under high-light conditions. Thus, categorization based on the similarity of fluorescence kinetics is an excellent way to identify the function of genes.
Ozaki
Hiroshi
H
Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa-shi, Chiba, 277-8562, Japan.
Ikeuchi
Masahiko
M
Ogawa
Teruo
T
Fukuzawa
Hideya
H
Sonoike
Kintake
K
D001483:Base Sequence; D002734:Chlorophyll / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D005453:Fluorescence; D015723:Gene Library; D005798:Genes, Bacterial; D007700:Kinetics; D016254:Mutagenesis, Insertional; D009154:Mutation; D010641:Phenotype; D010788:Photosynthesis / Q000235:genetics*; D013050:Spectrometry, Fluorescence; D046939:Synechocystis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*
D004269:DNA, Bacterial; D002734:Chlorophyll