Molecular characterization of two acetylcholinesterase cDNAs in Pediculus human lice.
Two cDNA sequences encoding Drosophila Ace-orthologous and -paralogous acetylcholinesterase precursors (AO- and AP-AChE precursors, respectively), were identified from the body louse, Pediculus humanus humanus L. In vitro inhibition studies with an insecticide-susceptible body louse strain exhibited a simplex inhibitory response of AChE. The I50 values of fenitroxon and carbaryl were estimated to be 2.2 and 1.9 microM for the susceptible lice, respectively. The mRNA level of AP-AChE gene was 3.1- and 9.3-fold higher than that of AO-AChE gene in the abdomen and the combined parts of the head and thorax, respectively, suggesting, due to its abundance, the potential significance of the AP-AChE isoform in Pediculus human lice in association with the efficacy of AChE-targeting pediculicides.
Lee
Si-Woo
SW
Department of Medical Entomology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan.
Kasai
Shinji
S
Komagata
Osamu
O
Kobayashi
Mutsuo
M
Agui
Noriaki
N
Kono
Yoshiaki
Y
Tomita
Takashi
T
D000110:Acetylcholinesterase / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D012721:Carbaryl / Q000633:toxicity; D018076:DNA, Complementary / Q000737:chemistry*; D019143:Evolution, Molecular; D005278:Fenitrothion / Q000031:analogs & derivatives / Q000633:toxicity; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50; D007306:Insecticides / Q000633:toxicity; D008969:Molecular Sequence Data; D010374:Pediculus / Q000187:drug effects / Q000235:genetics*; D016415:Sequence Alignment
D018076:DNA, Complementary; D007306:Insecticides; C007711:fenitrooxone; D000110:Acetylcholinesterase; D012721:Carbaryl; D005278:Fenitrothion