title: Gadobenate dimeglumine as a contrast agent for MRI of the mouse liver.
abstract: We investigated the characteristics and utility of gadobenate dimeglumine (Gd-BOPTA) for MRI of the mouse liver. Mice were imaged under isoflurane anesthesia using a T(1)-weighted, three-dimensional fast low-angle shot (3D FLASH) sequence before and after intravenous or subcutaneous injection of Gd-BOPTA, and the time course of the contrast effect was examined. The appropriate dose for subcutaneous injection was determined visually, and the inter- and intra-observer reproducibilities in liver volumetry were evaluated with and without contrast injection. When mice were imaged sequentially before and after Gd-BOPTA injection and isoflurane anesthesia was maintained throughout the experiment, a long-lasting contrast effect was noted in the liver. Subcutaneous injection caused delayed, but favorable, enhancement. Washout from the liver was definitely accelerated in conscious mice in comparison with anesthetized mice. Visual evaluation indicated that a dose of 0.1 mmol/kg was appropriate for clear delineation of the entire liver margin, and the application of Gd-BOPTA significantly improved the inter- and intra-observer reproducibilities of liver volumetry. In conclusion, the intravenous or subcutaneous injection of Gd-BOPTA has a favorable contrast effect for the mouse liver, resulting in clear visualization of the liver border and improved reproducibility of liver volumetry. The possible influence of anesthesia on the pharmacokinetics of a contrast agent should be considered in determining the optimal scan timing.
authors.affiliation: Department of Radiology, Institute of Medical Science, University of Tokyo, Tokyo, Japan. inoueys-tky@umin.ac.jp
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Inoue
authors.forename: Kohki
authors.initials: K
authors.lastname: Yoshikawa
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Nomura
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Izawa
authors.forename: Morio
authors.initials: M
authors.lastname: Shimada
authors.forename: Arinobu
authors.initials: A
authors.lastname: Tojo
authors.forename: Kuni
authors.initials: K
authors.lastname: Ohtomo
mesh_terms: D000818:Animals; D003287:Contrast Media; D005260:Female; D007091:Image Processing, Computer-Assisted; D007668:Kidney / Q000033:anatomy & histology* / Q000187:drug effects; D007700:Kinetics; D008099:Liver / Q000033:anatomy & histology* / Q000187:drug effects; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008536:Meglumine / Q000031:analogs & derivatives* / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D009942:Organometallic Compounds / Q000494:pharmacology*
chemical_list: D003287:Contrast Media; D009942:Organometallic Compounds; C064572:gadobenic acid; D008536:Meglumine