[HIF-1alpha expression and relationship involving tumor cell proliferation and angiogenesis in human breast carcinoma].
OBJECTIVE
To investigate the expression of hypoxia- inducible 1 alpha (HIF-1alpha) in human breast cancer and its relationship with vascular endothelial growth factor (VEGF) protein, proliferating cell nuclear antigen (PCNA ) protein, other tumor biomarkers and clinical pathologic factors.


METHODS
The immunohistochemical staining (SP) was used to measure the expression of HIF-1alpha, VEGF and PCNA in human breast fibroadenoma, usual hyperplasia and breast carcinoma.


RESULTS
HIF-1alpha was not found expressing in breast fibroadenoma and hyperplastic lesions. In contrast, the positive rate of HIF-1alpha was found in the ductal carcinoma in situ 55% (DCIS, 11/20) and the invasive breast carcinoma 85% (51/60). VEGF positivity in breast carcinoma was 81.3% (65/80). The total positive rate of PCNA in breast carcinoma was 75% (60/80), that in DCIS was 65% (13/20) and that in invasive carcinoma was 78.3% (47/66). Increased levels of HIF-1alpha were statistically significantly associated with increased expression of VEGF (r = 0.762, P <0.01) and PCNA (r = 0.693, P < 0. 01). Conclusion The upregulated expression of HIF-1alpha and VEGF in breast carcinoma has a close relationship with tumor angiogenesis, tumor cell proliferation, lymph node metastasis, ER status and stages of histology. This suggests that HIF-1alpha plays an important role in the carcinogenesis and progression of breast carcinoma; is wished to become a new target for radiotherapy, chemotherapy and biotherapy of tumor, which will offer the new ways to diagnosis and treatment of tumor.
Hao
Lang-song
LS
Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Wang
Geng
G
Qian
Kun
K
Luo
Ting
T
Li
Xiao-Jun
XJ
Wu
Xiao-Ting
XT
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology* / Q000503:physiopathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D008875:Middle Aged; D009389:Neovascularization, Pathologic; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
D014408:Biomarkers, Tumor; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D018809:Proliferating Cell Nuclear Antigen; D042461:Vascular Endothelial Growth Factor A