title: [The effects of tetrandrine on activity of collagenase derived from human hypertrophic scar].
abstract: OBJECTIVE
  To observe the effect of tetrandrine on activity of collagenase derived from human hypertrophic scar for the sake of clarifying the mechanism as tetrandrine acting on scar.
  
  
  METHODS
  The experimental concentration was controlled below that of cell proliferation inhibited, SDS-PAGE electrophoresis was adopted to separate collagenase from extracellular matrix, and then activated by trypsin analyzed the activity of collagenase with density scanning apparatus. At the same time quantity of extracellular collagen was measured using improved chloraseptine T oxidizing assay, moreover analyzed correlation between activity of collagenase and quantity of extracellular collagen.
  
  
  RESULTS
  In the concentration below the lever of inhibiting fibroblast proliferation, the total activity of collagenase could be significantly increased by tetrandrine with dosage-dependence associated with quantity of extracellular collagen reduced, which was much greater than that of triamcinolone.
  
  
  CONCLUSIONS
  Increasing activity of collagenase on degradation of collagen even in a lower concentration was one of the mechanisms of tetrandrine treating hypertrophic scar.
authors.affiliation: Department of Orthopedics, the First Aid Center of Chongqing, Chongqing 400014, China.
authors.forename: Zhi-dong
authors.initials: ZD
authors.lastname: Cao
authors.forename: Chong-rong
authors.initials: CR
authors.lastname: Shi
authors.forename: Chong-ben
authors.initials: CB
authors.lastname: Huang
authors.forename: Bang-chun
authors.initials: BC
authors.lastname: Li
authors.forename: Jing-yue
authors.initials: JY
authors.lastname: Gou
authors.forename: Yuan-lu
authors.initials: YL
authors.lastname: Liu
authors.forename: Kui
authors.initials: K
authors.lastname: Zhang
mesh_terms: D044182:Benzylisoquinolines / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D017439:Cicatrix, Hypertrophic / Q000378:metabolism* / Q000473:pathology; D017364:Collagenases / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology; D006801:Humans
chemical_list: D044182:Benzylisoquinolines; C009438:tetrandrine; D017364:Collagenases