[Separation of isomeric compounds by high speed countercurrent chromatography].
High speed countercurrent chromatography (HSCCC) was successfully used for the separation and preparation of isomeric compounds. The influence of various two-phase solvent systems on the resolution of the natural catechin isomers of (-) -epigallocatechin gallate (EGCG) and (-) -gallocatechin gallate (GCG) from green tea polyphenols and the synthetic isomeric compounds of para-, ortho-, meta-bromoanilline was investigated. The results indicated that the catechin isomers of (-) -EGCG and (-) -GCG can be isolated through two runs of preparative HSCCC with two-phase solvent system composed of n-hexane-ethyl acetate-water (1: 10: 10 in volume ratio). The upper organic phase was used as stationary phase and the lower aqueous phase as mobile phase, while the mobile phase was operated at a flow rate of 3.5 mL/min, and the apparatus rotated at 800 r/min. High performance liquid chromatographic analysis of (-) -EGCG and (-) -GCG revealed that the purity was all over 98%. The isomeric compounds of para-, ortho-, meta-bromoaniline can also be effectively isolated through a single run of preparative HSCCC with the mobile phase flow rate of 3.0 mL/min and the apparatus rotation speed of 800 r/min by using tetrachloromethane-chloroform-methanol-water (7: 3: 7: 3 in volume ratio) as two-phase solvent system, while the lower phase was selected as stationary phase and the upper phase as mobile phase.
Chen
Li
L
Institute for Drug Control of Hangzhou, Hangzhou 310014, China. c224@zju.edu.cn
Deng
Lijie
L
Chen
Ping
P
D000814:Aniline Compounds / Q000302:isolation & purification*; D002392:Catechin / Q000031:analogs & derivatives* / Q000302:isolation & purification; D003377:Countercurrent Distribution; D007536:Isomerism; D013662:Tea / Q000737:chemistry*
D000814:Aniline Compounds; D013662:Tea; C417940:gallocatechin gallate; D002392:Catechin; C045651:epigallocatechin gallate