Optimizing annealing temperature overcomes bias in bisulfite PCR methylation analysis.
Shen
Lanlan
L
Department of Leukemia, M.D. Anderson Cancer Center, Houston, TX 77030, USA. lshen@mdanderson.org
Guo
Yi
Y
Chen
Xinli
X
Ahmed
Saira
S
Issa
Jean-Pierre J
JP
D018899:CpG Islands; D019175:DNA Methylation; D016133:Polymerase Chain Reaction / Q000379:methods*; D013431:Sulfates; D013696:Temperature
D013431:Sulfates; C012036:sodium sulfate