title: Identifying four Trypanosoma cruzi I isolate haplotypes from different geographic regions in Colombia.
abstract: Trypanosoma cruzi has been classified into the groups T. cruzi I and T. cruzi II. The latter is subdivided into five smaller lineages based on multilocus enzyme electrophoresis and random amplified polymorphic DNA, designated as IIa-IIe, which shows correspondence with rRNA/mini-exon lineages. Twelve previously characterised T. cruzi isolates from different hosts, including humans, Didelphis marsupialis, and triatomines were analysed to establish genetic variability in T. cruzi group T. cruzi I isolates from different geographical regions of Colombia. DNA samples were sequenced based on the mini-exon gene intergenic region. Sequences were analysed using Clustal W, Staden 1.5 and MEGA3 software, and using reported sequences from the GenBank as reference. The genetic distances were analysed using Kimura's two-parameter model. The isolates' joint alignment was of 350bp, and the calculated nucleotide divergence was of 17.5%. The differences consisted of 23 transitions (7.2%), 14 transversions (4.4%) and 19 insertion-deletions (5.9%). The Colombian T cruzi I isolates revealed sufficient genetic variability for us to propose the existence of four haplotypes identified through single nucleotide polymorphism (SNP) and insertion/deletion found in the mini-exon gene's non-transcribed spacer intergenic region.
authors.affiliation: Centro de Investigaciones en Microbiología y Parasitología Tropical, Universidad de los Andes, Bogotá, Colombia. cherrera@uniandes.edu.co
authors.forename: Claudia
authors.initials: C
authors.lastname: Herrera
authors.forename: M Dolores
authors.initials: MD
authors.lastname: Bargues
authors.forename: Anabella
authors.initials: A
authors.lastname: Fajardo
authors.forename: Marleny
authors.initials: M
authors.lastname: Montilla
authors.forename: Omar
authors.initials: O
authors.lastname: Triana
authors.forename: Gustavo Adolfo
authors.initials: GA
authors.lastname: Vallejo
authors.forename: Felipe
authors.initials: F
authors.lastname: Guhl
mesh_terms: D000818:Animals; D001483:Base Sequence; D003105:Colombia; D005091:Exons / Q000235:genetics; D005843:Geography; D006239:Haplotypes / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D014349:Trypanosoma cruzi / Q000235:genetics* / Q000302:isolation & purification*