Taurine reduces caspase-8 and caspase-9 expression induced by ischemia in the mouse hypothalamic nuclei.
Taurine is a sulphur-containing amino acid abundant in the nervous system. It protects cells from ischemia-induced apoptosis, but the mechanism underlying this is not well established. The aim of our study was to explore the effects of taurine on two main pathways of apoptosis induced by ischemia: receptor-mediated and mitochondrial cell death. Brain slices containing the supraoptic (SON) and paraventricular (PVN) nuclei of the hypothalamus were incubated in vitro under control and simulated ischemic (oxygen-glucose deprivation for 30 min) conditions in the absence and presence of 20 mM taurine. Brain slices were harvested after the 180-min "postischemic" period and fixed in 4% paraformaldehyde. To estimate apoptosis, immunostaining was done for caspase-8 and caspase-9 in paraffin-embedded sections. Immunoreactive caspase-8 and caspase-9 cells were observed in SON and PVN in all experimental groups, but in the "ischemic" group the expression of caspase-8 and caspase-9 and the number of immunoreactive cells was significantly increased in both hypothalamic nuclei. Addition of taurine (20 mM) to the incubation medium induced a marked decrease in caspase-8 and caspase-9 immunoreactivity after ischemia in SON and PVN when compared with the taurine-untreated "ischemic" group. Taurine reduces ischemia-induced caspase-8 and caspase-9 expression, the key inductors of apoptosis in SON and PVN.
Taranukhin
A G
AG
Brain Research Center, University of Tampere Medical School, Tampere, Finland. Andrey.Taranukhin@uta.fi
Taranukhina
E Y
EY
Saransaari
P
P
Djatchkova
I M
IM
Pelto-Huikko
M
M
Oja
S S
SS
D000818:Animals; D053181:Caspase 8 / Q000378:metabolism*; D053453:Caspase 9 / Q000378:metabolism*; D007031:Hypothalamus / Q000187:drug effects* / Q000201:enzymology*; D007511:Ischemia / Q000201:enzymology*; D008297:Male; D051379:Mice; D013654:Taurine
D013654:Taurine; D053181:Caspase 8; D053453:Caspase 9