Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of DHNA synthetase from Geobacillus kaustophilus.
The aerobic Gram-positive bacterium Geobacillus kaustophilus is a bacillus species that was isolated from deep-sea sediment from the Mariana Trench. 1,4-Dihydroxy-2-naphthoate (DHNA) synthetase plays a vital role in the biosynthesis of menaquinone (vitamin K(2)) in this bacterium. DHNA synthetase from Geobacillus kaustophilus was crystallized in the orthorhombic space group C222(1), with unit-cell parameters a = 77.01, b = 130.66, c = 131.69 A. The crystal diffracted to a resolution of 2.2 A. Preliminary studies and molecular-replacement calculations reveal the presence of three monomers in the asymmetric unit.
Kanaujia
Shankar Prasad
SP
Bioinformatics Centre (Centre of Excellence in Structural Biology and Biocomputing), Indian Institute of Science, Bangalore 560 012, India.
Ranjani
Chellamuthu Vasuki
CV
Jeyakanthan
Jeyaraman
J
Baba
Seiki
S
Kuroishi
Chizu
C
Ebihara
Akio
A
Shinkai
Akeo
A
Kuramitsu
Seiki
S
Shiro
Yoshitsugu
Y
Sekar
Kanagaraj
K
Yokoyama
Shigeyuki
S
D001406:Bacillaceae / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D006836:Hydro-Lyases / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
D006836:Hydro-Lyases; C021853:naphthoate synthetase