title: Benzo(a)pyrene inhibits growth and functional differentiation of mouse bone marrow-derived dendritic cells. Downregulation of RelB and eIF3 p170 by benzo(a)pyrene.
abstract: In this study, we have investigated effects of benzo(a)pyrene (BP) on growth and functional differentiation of mouse bone marrow (BM)-derived dendritic cells (DC). 1 microM BP dramatically inhibited growth of BM cultured in the presence of granulocyte-macrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4). Although little alterations in surface expression of CD11c, major histocompatibility complex (MHC II), and CD86 molecules characteristic of mature DC were induced by BP, production of cytokines including IL-12, IL-10, and TNF-alpha, and allogeneic T cell stimulating ability were severely impaired. Some of the effects of BP were dependent on arylhydrocarbon receptor (AhR), because alpha-naphthoflavone, an AhR antagonist, suppressed the effects of BP on IL-12 production and T cell stimulating ability, but not on DC proliferation. Expression of RelB, a transcription factor necessary for DC differentiation and function, and eIF3 p170, a subunit of eukaryotic translation initiation factor (eIF)3, was reduced upon BP treatment.
authors.affiliation: Department of Biology, Changwon National University, Changwon, Kyungnam 641-773, South Korea.
authors.forename: Ju-Ae
authors.initials: JA
authors.lastname: Hwang
authors.forename: Jin-Ah
authors.initials: JA
authors.lastname: Lee
authors.forename: Seon-Woo
authors.initials: SW
authors.lastname: Cheong
authors.forename: Hyun-Joo
authors.initials: HJ
authors.lastname: Youn
authors.forename: Joo-Hung
authors.initials: JH
authors.lastname: Park
mesh_terms: D000818:Animals; D051940:B7-2 Antigen / Q000096:biosynthesis; D001564:Benzo(a)pyrene / Q000633:toxicity*; D001854:Bone Marrow Cells / Q000187:drug effects*; D039521:CD11c Antigen / Q000096:biosynthesis; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000187:drug effects*; D015536:Down-Regulation / Q000187:drug effects; D039621:Eukaryotic Initiation Factor-3 / Q000096:biosynthesis* / Q000235:genetics; D005434:Flow Cytometry; D005802:Genes, MHC Class II / Q000187:drug effects; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D007166:Immunosuppressive Agents; D007202:Indicators and Reagents; D015847:Interleukin-4 / Q000096:biosynthesis; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008807:Mice, Inbred BALB C; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification; D018336:Receptors, Aryl Hydrocarbon / Q000187:drug effects; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013723:Teratogens / Q000633:toxicity; D051841:Transcription Factor RelB / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D051940:B7-2 Antigen; D039521:CD11c Antigen; D016207:Cytokines; D039621:Eukaryotic Initiation Factor-3; D007166:Immunosuppressive Agents; D007202:Indicators and Reagents; D000072317:Polychlorinated Dibenzodioxins; D018336:Receptors, Aryl Hydrocarbon; C497594:Relb protein, mouse; D013723:Teratogens; D051841:Transcription Factor RelB; D015847:Interleukin-4; D001564:Benzo(a)pyrene; D012313:RNA; D016178:Granulocyte-Macrophage Colony-Stimulating Factor