title: Molecular phylogenetic diversity of the microbial community associated with a high-temperature petroleum reservoir at an offshore oilfield.
abstract: The microbial community and its diversity in production water from a high-temperature, water-flooded petroleum reservoir of an offshore oilfield in China were characterized by 16S rRNA gene sequence analysis. The bacterial and archaeal 16S rRNA gene clone libraries were constructed from the community DNA and, using sequence analysis, 388 bacterial and 220 archaeal randomly selected clones were clustered with 60 and 28 phylotypes, respectively. The results showed that the 16S rRNA genes of bacterial clones belonged to the divisions Firmicutes, Thermotogae, Nitrospirae and Proteobacteria, whereas the archaeal library was dominated by methanogen-like rRNA genes (Methanothermobacter, Methanobacter, Methanobrevibacter and Methanococcus), with a lower percentage of clones belonging to Thermoprotei. Thermophilic microorganisms were found in the production water, as well as mesophilic microorganisms such as Pseudomonas and Acinetobacter-like clones. The thermophilic microorganisms may be common inhabitants of geothermally heated specialized subsurface environments, which have been isolated previously from a number of high-temperature petroleum reservoirs worldwide. The mesophilic microorganisms were probably introduced into the reservoir as it was being exploited. The results of this work provide further insight into the composition of microbial communities of high-temperature petroleum reservoirs at offshore oilfields.
authors.affiliation: Institute of Applied Chemistry, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Shi-Zhong
authors.initials: SZ
authors.lastname: Yang
authors.forename: Bo-Zhong
authors.initials: BZ
authors.lastname: Mu
authors.forename: Zhao-Feng
authors.initials: ZF
authors.lastname: Rong
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics; D002681:China; D004275:DNA, Ribosomal / Q000032:analysis; D017753:Ecosystem; D019605:Euryarchaeota / Q000145:classification* / Q000235:genetics; D005618:Fresh Water / Q000382:microbiology; D015723:Gene Library; D019766:Genes, Archaeal; D005798:Genes, Bacterial; D020459:Genes, rRNA; D006358:Hot Temperature; D008697:Methane / Q000378:metabolism; D008969:Molecular Sequence Data; D010578:Petroleum; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004275:DNA, Ribosomal; D010578:Petroleum; D012336:RNA, Ribosomal, 16S; D008697:Methane