Comparative evaluation of different DNA extraction procedures from food samples.
Five methodologies for extracting DNA from food samples are described. The food products analyzed are from either soybean or maize. They were selected on the basis of the mechanical, thermal, and chemical treatments that they had been subjected to during industrial processing. DNA preparations were evaluated for purity, yield, and average fragment size. Two endogenous genes, soybean lectin gene and alcohol dehydrogenase gene (adh1), were used to assess the degree of DNA degradation at different stages of the transformation chain. The goal of this study was to determine the role that extraction methods play in DNA amplification in order to select the best protocol for a food sample. This comparative evaluation can be specifically useful for detection of genetically modified ingredients in a variety of food matrices.
Di Bernardo
G
G
Department of Experimental Medicine, Section of Biotechnologies and Molecular Biology, and CRISCEB, 2nd University of Naples, Via Costantinopoli 16, 80138 Naples, Italy.
Del Gaudio
S
S
Galderisi
U
U
Cascino
A
A
Cipollaro
M
M
D018744:DNA, Plant / Q000235:genetics* / Q000302:isolation & purification*; D005504:Food Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D052616:Solid Phase Extraction / Q000379:methods*; D013025:Glycine max / Q000235:genetics*; D013048:Specimen Handling / Q000379:methods; D003313:Zea mays / Q000235:genetics*
D018744:DNA, Plant