title: Natriuretic peptide responsive, cyclic guanosine monophosphate producing structures in the guinea pig bladder.
abstract: OBJECTIVE
  We examined the localization of natriuretic peptide responsive, cyclic guanosine monophosphate producing cells in the guinea pig bladder.
  
  
  METHODS
  The bladder was removed from male guinea pigs sacrificed by cervical dislocation. The lateral wall of the bladder was cut into strips 2 mm thick. The tissue pieces were incubated in the presence of human atrial natriuretic peptide, rat brain natriuretic peptide and C-type natriuretic peptide or the nitric oxide donor DEANO (diethylamine NONOate or 1,1-diethyl-2-hydroxy-2-nitrosohydrazine) (Sigma). Cyclic guanosine monophosphate immunoreactivity was localized using an antibody against formaldehyde fixed cyclic guanosine monophosphate.
  
  
  RESULTS
  Atrial natriuretic peptide and brain natriuretic peptide stimulated cyclic guanosine monophosphate synthesis in suburothelial interstitial cells, whereas C-type natriuretic peptide was not effective. In contrast, DEANO stimulated cyclic guanosine monophosphate synthesis in urothelial umbrella cells, suburothelial interstitial cells, muscle interstitial cells and neurons. The effect of atrial natriuretic peptide and brain natriuretic peptide was not inhibited by ODQ (1H-[1, 2, 4]oxadiazolo[4-3a]quinoxalin-1-one), an inhibitor of nitric oxide responsive soluble guanylyl cyclase.
  
  
  CONCLUSIONS
  To our knowledge our findings show for the first time a localized effect of atrial natriuretic peptide and brain natriuretic peptide to the suburothelial cells of the guinea pig bladder. These cells express the soluble guanylyl cyclase and particulate guanylyl cyclase-A isoforms. The specific physiological role of these cells is not known but it was suggested that they may be involved in the generation or modulation of sensation. The results imply a role for natriuretic peptide-cyclic guanosine monophosphate signaling in the processing of sensory information in the bladder.
authors.affiliation: European Graduate School of Neuroscience, Department of Psychiatry and Neuropsychology, Maastricht University, The Netherlands. j.devente@np.unimaas.nl
authors.forename: J
authors.initials: J
authors.lastname: de Vente
authors.forename: M
authors.initials: M
authors.lastname: Markerink-van Ittersum
authors.forename: J I
authors.initials: JI
authors.lastname: Gillespie
mesh_terms: D000818:Animals; D006152:Cyclic GMP / Q000378:metabolism*; D006168:Guinea Pigs; D006834:Hydrazines / Q000494:pharmacology; D008297:Male; D045265:Natriuretic Peptides / Q000494:pharmacology*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D046509:Tissue Culture Techniques; D001743:Urinary Bladder / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D019459:Urothelium / Q000187:drug effects / Q000378:metabolism / Q000473:pathology
chemical_list: D006834:Hydrazines; D045265:Natriuretic Peptides; D020030:Nitric Oxide Donors; C084362:uroguanylin; C084012:1,1-diethyl-2-hydroxy-2-nitrosohydrazine; D006152:Cyclic GMP