title: Comparison of transformation efficiency of piggyBac transposon among three different silkworm Bombyx mori Strains.
abstract: The transformation rate of three different strains of silkworm Bombyx mori was compared after the introduction of enhanced green fluorescence protein (EGFP)-encoding genes into the silkworm eggs by microinjection of a mixture of piggyBac vector and helper plasmid containing a transposase-encoding sequence. Although there were no significant differences among the three strains in the percentages of fertile moths in microinjected eggs (P=0.1258), the percentages of G(0) transformed moths in fertile moths and injected eggs were both significantly different (P=0.01368 and P=0.02398, respectively). The transformation rate of the Nistari strain (Indian strain) was significantly higher than that of the other two strains, Golden-yellow-cocoon (Vietnamese strain) and Jiaqiu (Chinese strain), which had similar rate. These results indicate that the transformation efficiency of the piggyBac-based system might vary with silkworm strains with different genetic backgrounds. The presence of endogenous piggyBac-like elements might be an important factor influencing the transformation efficiency of introduced piggyBac-derived vectors, and the diverse amount and activation in different silkworm strains might account for the significant differences.
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310029, China. bxzhong@zju.edu.cn
authors.forename: Boxiong
authors.initials: B
authors.lastname: Zhong
authors.forename: Jianying
authors.initials: J
authors.lastname: Li
authors.forename: Jin'e
authors.initials: J
authors.lastname: Chen
authors.forename: Jian
authors.initials: J
authors.lastname: Ye
authors.forename: Songdong
authors.initials: S
authors.lastname: Yu
mesh_terms: D000818:Animals; D012831:Bombyx / Q000145:classification / Q000235:genetics*; D002681:China; D017931:DNA Primers; D004251:DNA Transposable Elements / Q000235:genetics*; D005260:Female; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D007194:India; D010063:Ovum / Q000502:physiology; D010957:Plasmids; D016133:Polymerase Chain Reaction; D014170:Transformation, Genetic; D014744:Vietnam
chemical_list: D017931:DNA Primers; D004251:DNA Transposable Elements; D049452:Green Fluorescent Proteins