Enhanced heterologous production of eicosapentaenoic acid in Escherichia coli cells that co-express eicosapentaenoic acid biosynthesis pfa genes and foreign DNA fragments including a high-performance catalase gene, vktA.
Cellular eicosapentaenoic acid (EPA) makes up approximately 3% of total fatty acids in Escherichia coli DH5alpha, a strain that carries EPA biosynthesis genes (pEPADelta1). EPA was increased to 12% of total fatty acids when the host cell co-expressed the vector pGBM3::sa1(vktA), which carried the high-performance catalase gene, vktA. Where this vector was co-expressed, the transformant accumulated a large amount of VktA protein. However, the EPA production of cells carrying the vector, that included the insert lacking almost the entire vktA gene, was approximately 6%. This suggests that the retention of a large DNA insert in the vector and the accumulation of the resulting protein, but not the catalytic activity of VktA catalase, would potentially be able to increase the content of EPA.
Orikasa
Yoshitake
Y
Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo, 060-0810, Japan.
Ito
Yukiya
Y
Nishida
Takanori
T
Watanabe
Kazuo
K
Morita
Naoki
N
Ohwada
Takuji
T
Yumoto
Isao
I
Okuyama
Hidetoshi
H
D002374:Catalase / Q000235:genetics*; D004247:DNA / Q000378:metabolism*; D015118:Eicosapentaenoic Acid / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D015870:Gene Expression; D005798:Genes, Bacterial; D015183:Restriction Mapping
D004247:DNA; D015118:Eicosapentaenoic Acid; D002374:Catalase