Expression profiles of bovine genes in the rectoanal junction mucosa during colonization with Escherichia coli O157:H7.
A bovine-specific cDNA microarray was used to characterize gene expression in the bovine rectoanal junction mucosa in response to Escherichia coli O157:H7 colonization, and results were confirmed using quantitative real-time PCR. The results showed involvement of cell processes including immune response, cell structure/dynamics, signal transduction, intercellular communication, and metabolism.
Li
Jie
J
Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 83844-3052, USA.
Hovde
Carolyn J
CJ
D001003:Anal Canal / Q000382:microbiology; D000818:Animals; D002417:Cattle / Q000382:microbiology*; D019453:Escherichia coli O157 / Q000472:pathogenicity*; D020869:Gene Expression Profiling; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000382:microbiology*; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D012007:Rectum / Q000382:microbiology