title: Characterization of class I Newcastle disease virus isolates from Hong Kong live bird markets and detection using real-time reverse transcription-PCR.
abstract: Newcastle disease viruses isolated from Hong Kong live bird markets (LBMs) were not detected by a USDA-validated matrix gene real-time reverse transcription-PCR (RT-PCR) assay. Based upon phylogenetic analysis of the fusion gene, these viruses were related to lentogenic class I viruses found in U.S. LBMs and wild waterfowl. An alternative real-time RT-PCR assay which complements the matrix gene assay was developed to efficiently detect class I viruses.
authors.affiliation: Southeast Poultry Research Laboratory, USDA-ARS, 934 College Station Rd., Athens, GA 30605, USA.
authors.forename: L Mia
authors.initials: LM
authors.lastname: Kim
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: King
authors.forename: David L
authors.initials: DL
authors.lastname: Suarez
authors.forename: Chun W
authors.initials: CW
authors.lastname: Wong
authors.forename: Claudio L
authors.initials: CL
authors.lastname: Afonso
mesh_terms: D000818:Animals; D001717:Birds; D006723:Hong Kong; D008969:Molecular Sequence Data; D009521:Newcastle Disease / Q000821:virology*; D009522:Newcastle disease virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D014760:Viral Fusion Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014760:Viral Fusion Proteins