Inhibition of cyanide-insensitive respiration in Klebsiella oxytoca SYSU-011 by 8-hydroxyquinolone.
The inhibition of the cyanide (KCN)-insensitive respiration of Klebsiella oxytoca SYSU-011 by 8-hydroxyquinoline (8-HQ) was determined. Results showed that the profile of the rate of oxygen uptake of normal-grown and 8-HQ-grown K. oxytoca SYSU-011 was biphasic and similar, suggesting that 8-HQ did not inhibit the respiration of normal-grown K. oxytoca SYSU-011. A different biphasic KCN inhibition profile of respiration was observed for KCN-grown cells treated with and without 8-HQ. No decrease in respiration rate of KCN-grown cells and a 40% decrease in respiration rate of KCN-grown cells treated with 8-HQ were observed when KCN concentration was 10(-1) mM. Comparing differences of the profiles of oxygen uptake in KCN-grown cells with and without 8-HQ addition indicated that 8-HQ inhibited expression of the KCN-insensitive pathway carried out by nonheme oxidase. Greater inhibition of NADH oxidase activity by 2-n-heptyl-4-hydroxyquinoline-N-oxide from the cell membrane of the KCN-grown cells treated with 8-HQ, and more H2O2 production from these cells with than without 8-HQ, suggest that the function of the cyanide-insensitive pathway can stabilize the respiration of the cyanide-grown cells to prevent the production of H2O2.
Kao
Chih Ming
CM
Institute of Environmental Engineering, National SunYat-Sen University, Kaohsiung, Taiwan, ROC.
Hseu
You Cheng
YC
Huang
Yen Ling
YL
Tang
Peturs
P
Chen
Ssu Ching
SC
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000378:metabolism; D041121:Klebsiella oxytoca / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000037:antagonists & inhibitors; D009247:NADH, NADPH Oxidoreductases / Q000037:antagonists & inhibitors; D010088:Oxidoreductases / Q000037:antagonists & inhibitors; D010100:Oxygen / Q000378:metabolism; D010101:Oxygen Consumption / Q000187:drug effects*; D015125:Oxyquinoline / Q000494:pharmacology*; D011190:Potassium Cyanide / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D009097:Multienzyme Complexes; D015125:Oxyquinoline; D006861:Hydrogen Peroxide; D010088:Oxidoreductases; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases; D011190:Potassium Cyanide; D010100:Oxygen