[Detection of urothelial carcinoma of the urinary bladder by multicolor fluorescence in situ hybridization].
OBJECTIVE
Urothelial carcinoma of the urinary bladder is a common malignant neoplasm of the genitourinary system. This study was to analyze chromosome aberrations in urothelial carcinoma of the urinary bladder in Chinese, and evaluate the possibility and validity of multicolor fluorescence in situ hybridization (M-FISH) in detecting urothelial carcinoma of the urinary bladder.


METHODS
The probes of chromosome 3, 7, 17 centromeres and 9p21 region were labeled by random primer method. M-FISH was performed on interphase nuclei of 57 samples of urothelial carcinoma to analyze the correlations of chromosome aberration to diagnosis and pathology of urothelial carcinoma.


RESULTS
The rate of aneuploidy was 47.4% for chromosome 3, 50.9% for chromosome 7, 56.1% for chromosome 17, and 59.6% for chromosome 9p21 in urothelial carcinoma. All the aberrations had no correlations to tumor stage. The aberrations of chromosomes 3, 7, 17 were significantly correlated to pathologic grade (P<0.01). As using the 4 chromosome probes in combination, the sensitivity for detecting urothelial carcinoma of the urinary bladder was 54.4%.


CONCLUSIONS
M-FISH may be helpful for detecting urothelial carcinoma of the urinary bladder.
Zhang
Ye-Gui
YG
Department of Histology and Embryology, Anhui Medical University,Hefei, Anhui, PR China.
Bi
Xin-Gang
XG
Han
Ya-Ling
YL
Cai
Yan
Y
Xu
Xin
X
Wu
Yu-Peng
YP
Yang
Yi-Ling
YL
Ma
Jian-Hui
JH
Zhao
Ping
P
Jia
Xue-Mei
XM
Wang
Ming-Rong
MR
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000782:Aneuploidy; D002295:Carcinoma, Transitional Cell / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D002869:Chromosome Aberrations; D002886:Chromosomes, Human, Pair 17; D002893:Chromosomes, Human, Pair 3; D002897:Chromosomes, Human, Pair 7; D002899:Chromosomes, Human, Pair 9; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology