title: Carbonic anhydorase isoenzyme I (CA-I) concentration in feces and urine as a temporary marker of occult blood in beagle dogs.
abstract: This study was undertaken to investigate whether the concentration of carbonic anhydorase isoenzyme I (CA-I) in canine feces and urine is useful as a temporary marker of occult blood. Concentrations of CA-I were measured by enzyme-linked immunosorbent assay (ELISA). Fecal CA-I concentrations in 113 healthy beagle dogs (50 male and 63 female) of various ages ranged from 4.3 to 16.7 ng/g feces (mean; 7.0 +/- 2.9 ng/g feces). One milliliter of blood from 3 healthy beagle dogs was found to contain 1,047, 1,062 and 1,150 microg CA-I. The fecal CA-I concentrations of dogs receiving intragastric infusions of autologous blood (10 ml) were very low. However, the fecal CA-I concentrations of dogs receiving infusion of autologous blood (5 ml) into the ascending colon were very high. Detection of fecal CA-I would be useful for identifying dogs with hemorrhaging of the large intestine. Of 55 urinary samples collected from healthy beagle dogs by catheter, chemical tests for occult blood were negative in 44, but CA-I concentrations ranged from 1.8 to 12.6 ng/ml (mean; 6.9 +/- 5.4 ng/ml) by ELISA. The CA-I concentrations of the other 11 samples, which tested positive for occult blood on chemical testing, ranged from 41.2 to 525.0 ng/ml by ELISA. Although CA-I is not a specific marker of erythrocytes, CA-I may be used to detect occult blood in canine feces and urine until a specific immunological test kit using antibody for Hb is developed.
authors.affiliation: Laboratory of Immunology, College of Environmental and Health Sciences, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 229-8501, Japan.
authors.forename: Kouji
authors.initials: K
authors.lastname: Iguchi
authors.forename: Katsuhito
authors.initials: K
authors.lastname: Kawato
authors.forename: Tetsurou
authors.initials: T
authors.lastname: Seita
authors.forename: Takashi
authors.initials: T
authors.lastname: Kuribayashi
authors.forename: Tokihiro
authors.initials: T
authors.lastname: Shimada
authors.forename: Mariko
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Manabu
authors.initials: M
authors.lastname: Yamada
authors.forename: Shizuo
authors.initials: S
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000652:urine; D002256:Carbonic Anhydrases / Q000032:analysis* / Q000652:urine; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000201:enzymology*; D005260:Female; D007527:Isoenzymes / Q000032:analysis / Q000652:urine; D008297:Male; D009780:Occult Blood
chemical_list: D015415:Biomarkers; D007527:Isoenzymes; D002256:Carbonic Anhydrases