Analysis of carvedilol in human plasma using hydrophilic interaction liquid chromatography with tandem mass spectrometry.
A rapid, sensitive and selective method for the determination of carvedilol in human plasma was developed using hydrophilic interaction liquid chromatography with tandem mass spectrometry (HILIC-MS/MS). Carvedilol and cisapride (internal standard) were extracted from human plasma with methyl tert-butyl ether at basic pH and analyzed on an Atlantis HILIC Silica column with the mobile phase of acetonitrile-ammonium formate (50 mM, pH 4.5) (90:10, v/v). The analytes were detected using an electrospray ionization tandem mass spectrometry in the multiple-reaction-monitoring mode. The standard curve was linear (r=0.9998) over the concentration range of 0.1-200 ng/ml. The lower limit of quantification for carvedilol was 0.1 ng/ml using 50 microl plasma sample. The coefficient of variation and relative error for intra- and inter-assay at four QC levels were 1.6-4.5% and -6.4 to 4.8%, respectively. The absolute and relative matrix effect for carvedilol and cisapride were practically absent. The extraction recoveries of carvedilol and cisapride were 81.6 and 85.2%, respectively. This method was successfully applied to the bioequivalence study of carvedilol in humans.
Jeong
Dong Won
DW
Drug Metabolism and Bioanalysis Laboratory, College of Pharmacy, Wonkwang University, Shinyongdong, Iksan 570-749, Republic of Korea.
Kim
Young Hoon
YH
Ji
Hye Young
HY
Youn
Yu Seok
YS
Lee
Kang Choon
KC
Lee
Hye Suk
HS
D000328:Adult; D000959:Antihypertensive Agents / Q000097:blood*; D002138:Calibration; D002227:Carbazoles / Q000097:blood*; D000077261:Carvedilol; D002853:Chromatography, Liquid; D002945:Cisplatin / Q000032:analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D011412:Propanolamines / Q000097:blood*; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D012822:Silicon Dioxide; D012997:Solvents; D053719:Tandem Mass Spectrometry; D013810:Therapeutic Equivalency
D000959:Antihypertensive Agents; D002227:Carbazoles; D011412:Propanolamines; D012997:Solvents; D000077261:Carvedilol; D012822:Silicon Dioxide; D002945:Cisplatin