Detection and identification by PCR of a highly virulent phylogenetic subgroup among extraintestinal pathogenic Escherichia coli B2 strains.
Closely related Escherichia coli B2 strains O1:K1, O2:K1, O18:K1, and O45:K1 constitute a major subgroup causing extraintestinal infections. A DNA pathoarray analysis was used to develop a PCR specific for this subgroup that was included in the multiplex phylogenetic-grouping PCR method. Our PCR may serve to identify this virulent subgroup among different ecological niches.
Bidet
Philippe
P
Service de Microbiologie, Hôpital Robert Debré, 48 Bd Sérurier, 75395 Paris cedex 19, France. edouard.bingen@rdb.ap-hop-paris.fr.
Metais
Arnaud
A
Mahjoub-Messai
Farah
F
Durand
Lionel
L
Dehem
Marie
M
Aujard
Yannick
Y
Bingen
Edouard
E
Nassif
Xavier
X
Bonacorsi
Stéphane
S
D001483:Base Sequence; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D021521:Ribotyping; D014774:Virulence