Factors Influencing the Formation of MSCs APA Microcapsules.
This paper aims to produce alginate-(poly-l-lysine)- alginate(APA) microcapsules of MSCs(mesenchymal stem cell), and to investigate the influencing factors of cell microcapsules' formation. Bone marrow mesenchymal stem cells were enclosed within APA microcapsules by a high-voltage electrostatic microcapsules generator. The factors influencing the uniformity, integrity and the ratio of successful microcapsules were investigated. It showed that the cell density, the concentration of alginate solution and the purity of cell solution have a significant effect on the formation of microcapsules. When the cell density is 3&#215;106/ml, alginate concentration is 1.75%, centrifugal times is twice, perfect microcapsules with good sphericity, uniformity, and the diameter at 200 to 300&#956;m could be made.
Rong
Liu
L
Institute of Cryomedical Technology, University of Shanghai for Science and Technology1, Shanghai 200093,China (e-mail: ioasis@126.com).
Baoguo
Li
L
Huifeng
Ding
D
Tingting
Tang
T