title: Use of hepatocytes from Hoplias malabaricus to characterize the toxicity of a complex mixture of lipophilic halogenated compounds.
abstract: Organisms are continuously exposed to a plethora of anthropogenic toxicants daily released to the environment. In the present study, the effects of a mixture of halogenated organic compounds (HOCs) extracted from hepatic lipids were evaluated on the primary hepatocyte culture from fish Hoplias malabaricus. Cells were isolated through non-enzymatic perfusion protocol and cultured during 3 days to allow attachment. Two concentrations of the mixture of HOCs (10 ng ml(-1) [Mix10] and 50 ng ml(-1) [Mix50]) were tested in cells for 2 days by medium replacement. The control groups, with and without solvent (DMSO) were run in the same conditions. Both tested concentrations of HOCs increased the catalase and GST activities, but only the Mix50 increase the DNA damage and decreased the GSH concentration and cell viability. Lipid peroxidation increased in the Mix10 group, but it seems to be more a consequence of DMSO presence than the HOCs themselves. The DMSO at 0.1% increased the lipid peroxidation, GSH concentration, apoptosis and DNA damage. The present data suggest that DMSO interferes with the hepatocytes of H. malabaricus in culture and that the mixture of HOCs tested alters the redox state of the hepatocytes.
authors.affiliation: Departamento de Biologia Celular, Universidade Federal do Paraná, Cx. Postal 19031, CEP: 81.531-990, Curitiba, PR, Brazil.
authors.forename: F
authors.initials: F
authors.lastname: Filipak Neto
authors.forename: S M
authors.initials: SM
authors.lastname: Zanata
authors.forename: H C
authors.initials: HC
authors.lastname: Silva de Assis
authors.forename: D
authors.initials: D
authors.lastname: Bussolaro
authors.forename: M V M
authors.initials: MV
authors.lastname: Ferraro
authors.forename: M A F
authors.initials: MA
authors.lastname: Randi
authors.forename: J R M
authors.initials: JR
authors.lastname: Alves Costa
authors.forename: M M
authors.initials: MM
authors.lastname: Cestari
authors.forename: H
authors.initials: H
authors.lastname: Roche
authors.forename: C A
authors.initials: CA
authors.lastname: Oliveira Ribeiro
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002374:Catalase / Q000378:metabolism; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002849:Chromatography, Gas; D020552:Comet Assay; D045424:Complex Mixtures / Q000737:chemistry / Q000633:toxicity*; D004249:DNA Damage; D005399:Fishes / Q000502:physiology*; D005982:Glutathione Transferase / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects*; D006846:Hydrocarbons, Halogenated / Q000737:chemistry / Q000633:toxicity*; D006861:Hydrogen Peroxide / Q000378:metabolism; D020287:In Situ Nick-End Labeling; D015227:Lipid Peroxidation / Q000187:drug effects; D008055:Lipids / Q000737:chemistry; D010084:Oxidation-Reduction
chemical_list: D045424:Complex Mixtures; D006846:Hydrocarbons, Halogenated; D008055:Lipids; D006861:Hydrogen Peroxide; D002374:Catalase; D005982:Glutathione Transferase