title: Fitness cost of staphylococcal cassette chromosome mec in methicillin-resistant Staphylococcus aureus by way of continuous culture.
abstract: We examined the effect of introducing type I or IV staphylococcal cassette chromosome mec (SCCmec) elements on the growth yield of Staphylococcus aureus in glucose-limited continuous culture. Type I showed increased glucose consumption and ATP demand per gram of cells synthesized and decreased cell yield compared to those of the parent strain. In contrast, type IV SCCmec elements had no adverse energetic effect.
authors.affiliation: Department of Microbiology and Immunology, Otago School of Medical Sciences, University of Otago, P.O. Box 56, Dunedin, New Zealand.
authors.forename: Sui Mae
authors.initials: SM
authors.lastname: Lee
authors.forename: Miriam
authors.initials: M
authors.lastname: Ender
authors.forename: Rajan
authors.initials: R
authors.lastname: Adhikari
authors.forename: John M B
authors.initials: JM
authors.lastname: Smith
authors.forename: Brigitte
authors.initials: B
authors.lastname: Berger-Bächi
authors.forename: Gregory M
authors.initials: GM
authors.lastname: Cook
mesh_terms: D002876:Chromosomes, Bacterial / Q000235:genetics*; D003470:Culture Media; D005798:Genes, Bacterial; D005947:Glucose / Q000378:metabolism*; D008712:Methicillin / Q000494:pharmacology; D016106:Methicillin Resistance / Q000235:genetics*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics*
chemical_list: D003470:Culture Media; D005947:Glucose; D008712:Methicillin