title: Thrombelastographic method to quantify the contribution of factor XIII to coagulation kinetics.
abstract: Factor XIII (FXIII) plays a critical role in clot strength, and FXIII deficiency or excess is associated with hemorrhage or thrombosis, respectively. Our goal was to design a thrombelastography-based method to characterize the effects of FXIII on plasma clot strength. Normal human plasma was exposed to 0 or 200 mug/ml anti-FXIII antibodies for 20 min prior to celite activation and calcium addition. Other plasma had addition of fibrinogen (625 mg/dl)/FXIII (2 U/ml) or 30% dilution with hydroxyethyl starch before exposure to 0 or 200 mug/ml anti-FXIII antibodies. Thromboelastography was performed and data were collected until stable clot strength was observed. The exposure of normal plasma to anti-FXIII antibodies resulted in a significant (P < 0.05) decrease in clot strength (63%) compared with plasma without antibodies. Further samples exposed to anti-FXIII antibodies had clot strength no different from FXIII-deficient plasma. The FXIII-mediated clot strength varied between 44 and 50% in hypercoagulable and hypocoagulable plasma, respectively. In conclusion, the present investigation successfully demonstrated a novel method to detect the impact of FXIII activity in plasma samples. Further actuarial investigation will be required to determine the utility of this approach in the diagnosis and treatment of patients with either acquired FXIII deficiency or excess and concordant coagulopathy.
authors.affiliation: Department of Anesthesiology, The University of Alabama at Birmingham, Birmingham, Alabama 35249, USA. vnielsen@uab.edu
authors.forename: Vance G
authors.initials: VG
authors.lastname: Nielsen
authors.forename: James K
authors.initials: JK
authors.lastname: Kirklin
authors.forename: Hugh
authors.initials: H
authors.lastname: Hoogendoorn
authors.forename: Truitt C
authors.initials: TC
authors.lastname: Ellis
authors.forename: William L
authors.initials: WL
authors.lastname: Holman
mesh_terms: D000906:Antibodies / Q000494:pharmacology; D001696:Biomechanical Phenomena; D001777:Blood Coagulation; D005176:Factor XIII / Q000138:chemical synthesis / Q000276:immunology / Q000494:pharmacology*; D005177:Factor XIII Deficiency / Q000175:diagnosis / Q000276:immunology; D006801:Humans; D007700:Kinetics; D013916:Thrombelastography / Q000379:methods* / Q000592:standards; D019851:Thrombophilia
chemical_list: D000906:Antibodies; D005176:Factor XIII