Correlation of interferon-gamma production by peripheral blood mononuclear cells with childhood malnutrition and susceptibility to amebiasis.
The contribution of interferon-gamma (IFN-gamma) to immunity from amebiasis was assessed in a three-year prospective study of children 2-5 years of age in an urban slum of Dhaka, Bangladesh. IFN-gamma produced by peripheral blood mononuclear cells stimulated with soluble amebic antigen was measured upon enrollment. Thirty-one of the 209 enrolled children had Entamoeba histolytica-associated diarrhea. Children who produced higher than the median level of IFN-gamma (median = 580 pg/mL) had longer survival without E. histolytica diarrhea/dysentery (log rank test P = 0.03) and a reduction in the risk of E. histolytica diarrhea/dysentery by more than half (Cox proportional hazard regression = 0.45, P = 0.04). When adjusted for stunting, the association between IFN-gamma and the time to the first episode of E. histolytica-associated diarrhea remained marginally significant (Cox proportional hazard regression = 0.49, P = 0.07). We conclude that production of IFN-gamma is linked to nutritional status and predicts future susceptibility to symptomatic amebiasis.
Haque
Rashidul
R
International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh.
Mondal
Dinesh
D
Shu
Jianfen
J
Roy
Shantanu
S
Kabir
Mamun
M
Davis
Andrea N
AN
Duggal
Priya
P
Petri
William A
WA
D000818:Animals; D000886:Anthropometry; D001459:Bangladesh; D002675:Child, Preschool; D015331:Cohort Studies; D003967:Diarrhea / Q000097:blood / Q000276:immunology / Q000469:parasitology; D004748:Entamoeba histolytica / Q000276:immunology*; D004749:Entamoebiasis / Q000097:blood / Q000276:immunology* / Q000469:parasitology; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000097:blood / Q000276:immunology; D015848:Interleukin-5 / Q000096:biosynthesis / Q000276:immunology; D053208:Kaplan-Meier Estimate; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008297:Male; D044342:Malnutrition / Q000097:blood / Q000276:immunology*; D011204:Poverty Areas; D016016:Proportional Hazards Models; D011446:Prospective Studies; D014505:Urban Population
D015848:Interleukin-5; D007371:Interferon-gamma