Chitosan-phosphorylated chitosan polyelectrolyte complex hydrogel as an osteoblast carrier.
To simulate extra-cellular matrix, a novel three-dimensional scaffold of polyelectrolyte complex (PEC) hydrogel as an osteoblast carrier was synthesized. First, chitosan, a natural glycosaminoglycan, was modified by phosphorylation to obtain a water-soluble phosphorylated chitosan (P-content: 10.7 mass%). The PEC hydrogel was then formed from equal volumes of 0.173 mass% phosphorylated chitosan in water and 1 mass% chitosan in 1% (V/V) acetic acid solution. Rat osteoblasts were seeded in the hydrogel. The PEC hydrogel had a three-dimensional hierarchically-porous structure and good cytobiocompatibility for osteoblasts in vitro. It is concluded that the PEC hydrogel is a promising material as an osteoblast carrier.
Li
Quan-Li
QL
Department of Stomatology, Anhui Medical University, Hefei, China.
Chen
Zhi-Qing
ZQ
Darvell
Brian W
BW
Liu
Lai-Kui
LK
Jiang
Hong-Bing
HB
Zen
Quan
Q
Peng
Qian
Q
Ou
Guo-Min
GM
D000818:Animals; D018914:Cells, Immobilized; D048271:Chitosan / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry / Q000494:pharmacology; D020100:Hydrogels / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D008422:Materials Testing; D010006:Osteoblasts / Q000737:chemistry / Q000187:drug effects*; D051381:Rats; D023822:Tissue Engineering
D020100:Hydrogels; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D048271:Chitosan