Sonoreactor-based technology for fast high-throughput proteolytic digestion of proteins.
Fast (120 s) and high-throughput (more than six samples at once) in-gel trypsin digestion of proteins using sonoreactor technology has been achieved. Successful protein identification was done by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, MALDI-TOF-MS. Specific identification of the adenylylsulphate reductase alfa subunit from a complex protein mixture from Desulfovibrio desulfuricans ATCC 27774 was done as a proof of the methodology. The new sample treatment is of easy implementation, saves time and money, and can be adapted to online procedures and robotic platforms.
Rial-Otero
R
R
REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Quinta da Torre, 2829-516 Monte de Caparica, Portugal.
Carreira
R J
RJ
Cordeiro
F M
FM
Moro
A J
AJ
Fernandes
L
L
Moura
I
I
Capelo
J L
JL
D001426:Bacterial Proteins / Q000737:chemistry; D003198:Computer Simulation; D045285:Desulfovibrio desulfuricans / Q000201:enzymology; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000737:chemistry; D010447:Peptide Hydrolases; D011506:Proteins / Q000737:chemistry*; D013010:Sonication; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001426:Bacterial Proteins; D011506:Proteins; D050862:Oxidoreductases Acting on Sulfur Group Donors; C019618:adenylylsulfate reductase; D010447:Peptide Hydrolases