A flow injection sampling resonance light scattering system for total protein determination in human serum.
A novel flow injection method with resonance light scattering detection was developed for the determination of total protein concentrations. This method is based on the enhancement of RLS signals from Methyl Blue (MB) by protein. The enhanced RLS intensities at 333 nm, in a pH 4.1 acidic aqueous solution, were proportional to the protein concentration over the range 2.0-37.3 and 1.0-36.0 microg ml-1 for human serum albumin (HSA) and bovine serum albumin (BSA), respectively. The corresponding limits of detection (3sigma) of 45 ng ml-1 for HSA and 80 ng ml-1 for BSA were attained. The method was successfully applied to the quantification of total proteins in human serum samples, the maximum relative error is less than 1% and the recovery is between 98% and 102%. The sample throughput was 60 h-1.
Dong
Lijun
L
Department of Chemistry, Lanzhou University, Lanzhou 730000, PR China.
Li
Ying
Y
Zhang
Yaheng
Y
Chen
Xingguo
X
Hu
Zhide
Z
D000596:Amino Acids; D000818:Animals; D001557:Benzenesulfonates / Q000737:chemistry; D001798:Blood Proteins / Q000032:analysis*; D002417:Cattle; D017022:Flow Injection Analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008027:Light; D012044:Regression Analysis; D012542:Scattering, Radiation; D013997:Time Factors
D000596:Amino Acids; D001557:Benzenesulfonates; D001798:Blood Proteins; C414357:Methyl Blue