Discrimination of Cryptosporidium species by denaturing gradient gel electrophoresis.
Denaturing gradient gel electrophoresis (DGGE) was used for the discrimination of three species and one genotype of the protozoan parasite Cryptosporidium: the C. parvum, C. andersoni, C. muris, and C. muris Japanese field mouse genotype. A set of primers specific for the 18S rRNA gene of Cryptosporidium was used in the DGGE; consequently, the four strains showed different banding patterns. This is a potentially convenient and precise method for the discrimination of Cryptosporidium spp.
Satoh
Masaaki
M
Laboratory of Animal Health and Management, Graduate School of Agricultural Science, Tohoku University, Naruko-Onsen, Osaki City, Miyagi, 989-6711, Japan.
Nakai
Yutaka
Y
D000818:Animals; D001483:Base Sequence; D003458:Cryptosporidium / Q000145:classification* / Q000235:genetics*; D016172:DNA Fingerprinting; D016054:DNA, Protozoan / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005838:Genotype; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation; D012337:RNA, Ribosomal, 18S / Q000235:genetics*
D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S