Involvement of microglia in the ethanol-induced neuropathic pain-like state in the rat.
Central mechanisms of neuropathy induced by chronic ethanol treatment are almost unknown. In this study, rats were treated with ethanol-diet for 72 days. Mechanical hyperalgesia was observed during ethanol consumption, even after ethanol withdrawal. Under these conditions, a microglial marker ionized calcium-binding adaptor molecule 1-, but not a neuron marker microtuble associated protein-2-, like immunoreactivies were increased in the rat spinal cord. Furthermore, hypertrophy of microglia was clearly observed following chronic ethanol consumption. These findings support the idea that the activation and hypertrophy of microglia in the spinal cord may be, at least in part, associated with in the induction of ethanol-dependent neuropathic pain-like state.
Narita
Minoru
M
Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan. narita@hoshi.ac.jp
Miyoshi
Kan
K
Narita
Michiko
M
Suzuki
Tsutomu
T
D000344:Afferent Pathways / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D020269:Alcoholic Neuropathy / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000187:drug effects / Q000378:metabolism; D002492:Central Nervous System Depressants / Q000009:adverse effects; D000431:Ethanol / Q000009:adverse effects; D006930:Hyperalgesia / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology; D006984:Hypertrophy / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology; D008297:Male; D008840:Microfilament Proteins; D017628:Microglia / Q000187:drug effects* / Q000473:pathology; D009475:Neurons, Afferent / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D010147:Pain Measurement / Q000187:drug effects; D017288:Pain Threshold / Q000187:drug effects / Q000502:physiology; D010525:Peripheral Nerves / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D051381:Rats; D011916:Rats, Inbred F344; D013116:Spinal Cord / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology*; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
C100745:Aif1 protein, rat; D015415:Biomarkers; D002135:Calcium-Binding Proteins; D002492:Central Nervous System Depressants; D008840:Microfilament Proteins; D000431:Ethanol