Photodynamic therapy against cyanobacteria.
This study explores the use of photosensitizers and reactive oxygen species (ROS) to limit growth of cyanobacteria. We chose 12 phthalocyanines, tetraphenol porphyrine, and methylene blue as compounds producing singlet oxygen. Hydrogen peroxide was chosen as another source of ROS. These compounds were tested using algal toxicity tests in microplates on three cultures of green algae (Pseudokirchneriella subcapitata, Scenedesmus quadricauda, and Chlorella kessleri) and on three cultures of cyanobacteria (Synechococcus nidulans, Microcystis incerta, and Anabaena sp.). Results indicate that photosensitizers and singlet oxygen could be highly toxic for some selected phytoplankton species. Green alga Scenedesmus quadricauda was highly sensitive (EC50 = 0.07 mg/L) to compounds producing singlet oxygen, although it was not sensitive to hydrogen peroxide, which was about 10 times more toxic for cyanobacteria. We conclude that the compounds producing hydroxyl radical species seems to be more promising to treat cyanobacterial blooms than the compounds producing the singlet oxygen.
Drábková
M
M
Research Centre for Environmental Chemistry and Ecotoxicology (RECETOX), Masaryk University, Kamenice 126/3, 625 00 Brno, Czech Republic.
Marsálek
B
B
Admiraal
W
W
D000460:Chlorophyta / Q000187:drug effects* / Q000254:growth & development / Q000528:radiation effects; D000458:Cyanobacteria / Q000187:drug effects* / Q000254:growth & development / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D006861:Hydrogen Peroxide / Q000494:pharmacology / Q000633:toxicity; D017665:Hydroxyl Radical / Q000378:metabolism; D008751:Methylene Blue / Q000494:pharmacology; D010777:Photochemistry; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology* / Q000633:toxicity; D010839:Phytoplankton / Q000187:drug effects* / Q000254:growth & development / Q000528:radiation effects; D011166:Porphyrins / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000494:pharmacology* / Q000633:toxicity; D026082:Singlet Oxygen / Q000378:metabolism; D018675:Toxicity Tests
D017319:Photosensitizing Agents; D011166:Porphyrins; D017382:Reactive Oxygen Species; D026082:Singlet Oxygen; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide; D008751:Methylene Blue