[Vitro culture of blastocystis hominis in medium DMEM].
OBJECTIVE
To establish the vitro culture of Blastocystis hominis (B. h) in medium DMEM for the further research on diagnosis, life cycle and pathogenicity of this intestinal protoza.


METHODS
The growth, reproduction and relevant factors of B . h under different culture conditions including sorts and concentrations of serum, pHs and number of inoculation were compared.


RESULTS
Conditions for the continuously anaerobic culture of B. h in medium DMEM were as follows: the number of inoculation were no less than 10(5) cells per tube, pHs ranged 7.0 - 8.0, concentrations of calf serum (or human serum and horse serum) ranged 10% - 30% , antibiotics and Amphotericin B should be added, subculturing could be choose at the each peaking-day 3,6 or 5 at 37 degrees C.


CONCLUSIONS
The medium DMEM could be used in diagnosis and continuously vitro culture for B. h.
Zhang
Xu
X
Key Laboratory of Environment and Genes Related to Diseases, Ministery of Education, Xi'an Jiaotong University, Xi'an 710061, China.
Qiao
Ji-ying
JY
Da
Rong
R
Li
Ya-qing
YQ
Yao
Fan-rong
FR
D000818:Animals; D016845:Blastocystis hominis / Q000254:growth & development* / Q000502:physiology; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D012098:Reproduction / Q000502:physiology
D003470:Culture Media