Online phototransformation-flow injection chemiluminescence determination of triclosan.
A highly selective and sensitive chemiluminescence method for the determination of triclosan is proposed. The method is based on the phototransformation of triclosan to a light-emitting precursor in the presence of fluorescein in alkaline medium and the chemiluminescence reaction is then triggered by strong base or oxidants such as N-bromosuccinimide. Based on this reaction an online phototransformation-flow injection manifold was developed, in which the photoreactor comprises a 150-cm-long x 0.8-mm-i.d. piece of PTFE tubing coiled around a 25-W fluorescent lamp, and the phototransformed products were then injected into a carrier stream of borate buffer. After mixing with the oxidant stream the produced light was detected by a photomultiplier. A wide calibration range from 8.0 x 10(-8) to 1.0 x 10(-4) mol L(-1) was obtained under the optimized conditions, and the detection limit was as low as 5.0 x 10(-8) mol L(-1). The whole process of analysis, including the online phototransformation and subsequent chemiluminescence detection, could be completed in 6 min. Most of the foreign substances tested showed high tolerance levels, and the proposed method was directly applied to the determination of triclosan in toothpaste samples without any pre-separation procedure. Figure Schematic representation of the phototransformation of triclosan and subsequent chemiluminescence reaction.
Song
Shujuan
S
School of Chemical and Material Engineering, Southern Yangtze University, Wuxi 214122, China.
Song
Qi Jun
QJ
Chen
Zhongliang
Z
D000891:Anti-Infective Agents, Local / Q000032:analysis*; D002138:Calibration; D017022:Flow Injection Analysis / Q000379:methods*; D049449:Luminescence; D010777:Photochemistry; D012680:Sensitivity and Specificity; D013501:Surface-Active Agents / Q000737:chemistry; D014260:Triclosan / Q000032:analysis*
D000891:Anti-Infective Agents, Local; D013501:Surface-Active Agents; D014260:Triclosan