Enhancement of ethanol production by promoting surface contact between starch granules and arming yeast in direct ethanol fermentation.
The specific ethanol production rate of raw starch by arming yeast cells displaying alpha-amylase and glucoamylase increased significantly when the cells and starch granules settled together. The specific ethanol production rate also increased when the size distribution of starch granules was almost same as that of the yeast cells. These results indicate that the surface contact between starch granules and yeast cells is important for increasing the apparent specific activity of alpha-amylase, which was the rate-limiting factor of the direct fermentation.
Khaw
Teik Seong
TS
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Katakura
Yoshio
Y
Ninomiya
Kazuaki
K
Moukamnerd
Churairat
C
Kondo
Akihiko
A
Ueda
Mitsuyoshi
M
Shioya
Suteaki
S
D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media / Q000737:chemistry; D000431:Ethanol / Q000378:metabolism*; D008863:Microspheres; D010316:Particle Size; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013213:Starch / Q000737:chemistry* / Q000378:metabolism*; D013499:Surface Properties
D003470:Culture Media; D000431:Ethanol; D013213:Starch