[Lectins modulate the cytostatic action of the composite bioregulators-conjugates of azaanalogues of pyrimidine and phenazine-1-carboxilic acid in Bacillus subtilis test-system].
The idea of the work was to study a combined effect of some lectins (Con A, PHA, STA, WGA, SNA, VAA) and new composite bioregulators (PhCA-1 and azapyrimidine derivatives) on growth of Bacillus subtilis cells in order to elucidate cell targets sensitive to lectin's activity. Study of combined effects of high- and low-molecular bioregulators may also be the subject of practical interest with a prospect of obtaining new bacterio-and carcinostatic drugs. Using B. subtilis mutants it was shown that lectins can modulate the cytostatic effect of initial substanses and their pyrimidine derivatives in the range: phenazine < PhCA-1 < 6-azacytosine derivative of PhCA-1 < 6-azauracyl derivative of PhCA-1. This modulating effect was absent in recP mutant, which has lost the ability to produce its own bacterial lectin, demonstrating its possible role as a mediator. The antagonistic effect of all plant lectins under study on cytostatic action of <6-azauracyl derivative of PhCA-1 in rec+ culture was observed as the result of possible competition for some common target. As both the B. subtilis lectin and the low-molecular bioregulator can inhibit transcription in vitro, it is supposed that their common target may be the DNA-dependent synthesis of RNA.
Karpova
I S
IS
Pal'chykovs'ka
L H
LH
Korets'ka
N V
NV
Platonov
M O
MO
Kovalenko
E O
EO
Het'man
K I
KI
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001372:Aza Compounds / Q000737:chemistry / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D004261:DNA Replication / Q000187:drug effects; D004269:DNA, Bacterial / Q000235:genetics; D004357:Drug Synergism; D010619:Phenazines / Q000737:chemistry / Q000494:pharmacology; D037121:Plant Lectins / Q000737:chemistry / Q000494:pharmacology*; D011743:Pyrimidines / Q000737:chemistry / Q000494:pharmacology*; D014158:Transcription, Genetic
D000900:Anti-Bacterial Agents; D001372:Aza Compounds; D004269:DNA, Bacterial; D010619:Phenazines; D037121:Plant Lectins; D011743:Pyrimidines; C037165:1-phenazinecarboxylic acid; C030986:pyrimidine