Microarray analysis of apple gene expression engaged in early fruit development.
To evaluate gene expressions mostly engaged in early development of apple fruit, we performed the identification of transcripts differentially expressed in young fruit by using microarrays spotted with 6,253 cDNAs collected from young and mature apple fruits of the cultivar Fuji (Malus domestica Borkh. cv. Fuji). A total of 3,484 cDNAs out of 6,253 were selected after quality control of microarray spots and analyzed for differential gene expression patterns between young fruit and other tissues (mature fruit, leaf and flower). Among them, 192 cDNAs displayed a signal value higher than twofold in young fruit compared with other tissues. Blast analysis of the 192 cDNA clones identified 88 non-redundant groups encoding proteins with known function and 50 non-redundant groups with unknown function. The putative protein products were classified into the following categories: photosynthesis (16.7%), protein synthesis (12.3%), cell proliferation and differentiation (10.9%), cell enlargement (5.8%), metabolism (8.0%), stress response (7.2%), others (2.9%), and unknown functions (32.2%). Furthermore, confirming the microarray data by reverse transcription-polymerase chain reaction revealed that the wide range of transcripts differentially expressed in young fruit was expressed in other organs but not in the mature fruit. The data presented suggested that apple fruit development depends on the tight regulation of the expression of a number of genes, which are also expressed in other organs.
Lee
Young-Pyo
YP
Biotech Application Team, Dongbu Advanced Research Institute, Daejeon 305-708, South Korea.
Yu
Gyung-Hee
GH
Seo
Young Sam
YS
Han
Sang Eun
SE
Choi
Yeon-Ok
YO
Kim
Daeil
D
Mok
Il-Gin
IG
Kim
Woo Taek
WT
Sung
Soon-Kee
SK
D005638:Fruit / Q000235:genetics* / Q000254:growth & development*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D027845:Malus / Q000235:genetics* / Q000254:growth & development*; D020411:Oligonucleotide Array Sequence Analysis; D013997:Time Factors