title: Antibody isotype responses to paramyosin, a vaccine candidate for schistosomiasis, and their correlations with resistance and fibrosis in patients infected with Schistosoma japonicum in Leyte, The Philippines.
abstract: We examined whether antibody isotype responses to paramyosin (PM), a vaccine candidate for schistosomiasis, are associated with age-dependent resistance and pathology in liver fibrosis using human sera collected from 139 individuals infected with Schistosoma japonicum in Leyte, The Philippines. We report that IgA and IgG3 responses to PM showed a positive correlation with age and that the epitopes responsible were localized predominantly within the N-terminal half of PM. In addition, the IgG3 response to PM was associated with serum level of procollagen-III-peptide (P-III-P), an indicator of progression of liver fibrosis. These results imply that IgG3 against PM may not only provoke age-dependent resistance to S. japonicum infection but also enhance liver fibrosis. In contrast, levels of IgE to PM and to multiple PM fragments showed a negative correlation with P-III-P level. Thus, in contrast to IgG3, increases in PM-specific IgE may contribute to suppression of liver pathogenesis in schistosomiasis.
authors.affiliation: Department of Molecular and Cellular Parasitology, Juntendo University School of Medicine, Tokyo, Japan. tnara@med.juntendo.ac.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nara
authors.forename: Kyoichi
authors.initials: K
authors.lastname: Iizumi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ohmae
authors.forename: Orlando S
authors.initials: OS
authors.lastname: Sy
authors.forename: Soichi
authors.initials: S
authors.lastname: Tsubota
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Inaba
authors.forename: Akiko
authors.initials: A
authors.lastname: Tsubouchi
authors.forename: Masanobu
authors.initials: M
authors.lastname: Tanabe
authors.forename: Somei
authors.initials: S
authors.lastname: Kojima
authors.forename: Takashi
authors.initials: T
authors.lastname: Aoki
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D002648:Child; D015331:Cohort Studies; D024141:Collagen Type IV / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D005260:Female; D006801:Humans; D007132:Immunoglobulin Isotypes / Q000276:immunology*; D008103:Liver Cirrhosis / Q000276:immunology* / Q000469:parasitology / Q000517:prevention & control; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000276:immunology; D010679:Philippines; D011347:Procollagen / Q000276:immunology; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000276:immunology* / Q000469:parasitology / Q000517:prevention & control; D018709:Statistics, Nonparametric; D014335:Tropomyosin / Q000235:genetics / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D024141:Collagen Type IV; D000939:Epitopes; D007132:Immunoglobulin Isotypes; D010446:Peptide Fragments; D011347:Procollagen; D014335:Tropomyosin; C041876:procollagen Type III-N-terminal peptide; D012313:RNA