Construction of an infectious cDNA clone for a Brazilian prototype strain of dengue virus type 1: characterization of a temperature-sensitive mutation in NS1.
To help understand the mechanism of pathogenesis of dengue virus (DV), we set out to create an infectious cDNA of the Brazilian prototype strain of DV serotype 1 (DV1-BR/90). PCR-amplified fragments of DV1-BR/90 cDNA were readily assembled into a subgenomic cDNA that could be used to produce replicating RNAs (replicons), lacking the structural protein-encoding regions of the genome. However, assembly of a cDNA capable of producing infectious virus was only possible using a bacterial artificial chromosome plasmid, indicating that DV1 sequences were especially difficult to propagate in E. coli. While characterizing our cDNA we discovered a fortuitous temperature-sensitive mutation in the NS1 encoding region. Using our infectious cDNA and a renilla luciferase-expressing replicon we were able to demonstrate that this mutation produced a defect in RNA replication at 37 degrees C, demonstrating that the DV1 NS1 protein plays an essential role in RNA replication.
Suzuki
Ryosuke
R
Department of Pathology, University of Texas Medical Branch, 3.206B Mary Moody Northen Pavilion, 301 University Boulevard, Galveston, TX 77555-0436, USA.
de Borba
Luana
L
Duarte dos Santos
Claudia N
CN
Mason
Peter W
PW
D000328:Adult; D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D022202:Chromosomes, Artificial, Bacterial; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D003715:Dengue / Q000821:virology; D003716:Dengue Virus / Q000235:genetics* / Q000502:physiology; D020043:Genes, Essential / Q000235:genetics; D016679:Genome, Viral / Q000235:genetics*; D006801:Humans; D008297:Male; D009154:Mutation / Q000235:genetics*; D010641:Phenotype; D020815:Polyproteins / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013696:Temperature; D014709:Vero Cells; D017361:Viral Nonstructural Proteins / Q000235:genetics*; D014779:Virus Replication
D018076:DNA, Complementary; D004279:DNA, Viral; C082515:NS1 protein, dengue-1 virus; D020815:Polyproteins; D012367:RNA, Viral; D017361:Viral Nonstructural Proteins