title: The detection of serum anti-p53 antibodies from patients with gastric carcinoma in China.
abstract: BACKGROUND
  Gastric carcinoma is one of the most frequently occurring cancers. The aim of this research was to increase the detection efficiency of anti-p53 antibodies in the sera of patients with gastric carcinoma and to improve the diagnosis for patients with gastric carcinoma.
  
  
  METHODS
  We prepared phage-displayed peptide DO7 and established an enzyme-linked immunosorbent assay method to detect the anti-p53 antibodies. We detected the anti-p53 antibodies of 61 patients with gastric carcinoma using the method and our previous ELISA method assisted by the recombinant wild-type human p53 protein to detect the anti-p53 antibodies. We studied the correlation between the anti-p53 antibodies and the clinicopathological data including sex, age, carcinoembryonic antigen, tumor size, tumor TNM staging, and lymph-node status.
  
  
  RESULTS
  The anti-p53 antibodies positive rate for patients with gastric carcinoma was increased (31.1%, 19/61) through the combination of p53-ELISA and phage-ELISA. We found that the positive anti-p53 antibodies correlated significantly with tumor size (P=0.047). The combination of the anti-p53 antibodies and carcinoembryonic antigen could improve the diagnosis for patients with gastric carcinoma.
  
  
  CONCLUSIONS
  This approach indicated an increased anti-p53 antibodies positive rate for patients with gastric carcinoma and provided a useful marker for clinical diagnosis for patients with gastric carcinoma.
authors.affiliation: Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin Province, PR China.
authors.forename: Lin-Lin
authors.initials: LL
authors.lastname: Qiu
authors.forename: Pan-Yu
authors.initials: PY
authors.lastname: Hua
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Ye
authors.forename: Yong-Cai
authors.initials: YC
authors.lastname: Wang
authors.forename: Tian
authors.initials: T
authors.lastname: Qiu
authors.forename: Hui-Zheng
authors.initials: HZ
authors.lastname: Bao
authors.forename: Li
authors.initials: L
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000912:Antibodies, Neoplasm / Q000097:blood*; D001323:Autoantibodies / Q000097:blood*; D014408:Biomarkers, Tumor / Q000097:blood*; D015153:Blotting, Western; D002272:Carcinoembryonic Antigen / Q000097:blood; D016022:Case-Control Studies; D002681:China; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D019151:Peptide Library; D013274:Stomach Neoplasms / Q000276:immunology* / Q000473:pathology; D016159:Tumor Suppressor Protein p53 / Q000276:immunology*
chemical_list: D000912:Antibodies, Neoplasm; D001323:Autoantibodies; D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D019151:Peptide Library; D016159:Tumor Suppressor Protein p53