Maximum a posteriori (MAP) estimator for polymerase chain reaction (PCR) processes.
A maximum a posteriori (MAP) estimator for polymerase chain reaction (PCR) assays is presented. The estimation relies on the replication efficiencies of the PCR assay and the observed end-point concentration of the PCR product at an arbitrary cycle. This derivation is carried out in view of the stochastic progression of the amplicons, and the assumption that the end-point concentrations for any particular initial value have a jointly Gaussian distribution. In addition, we provide an extension for the estimator which can be applied to various quantitative PCR assays.
Kakavand
Hossein
H
Dept. of Electr. Eng., Stanford Univ., CA, USA.
O'brien
Deirdre
D
Hassibi
Arjang
A
Lee
Thomas H
TH