title: Fermentative production of (R)-(-)-3-hydroxybutyrate using 3-hydroxybutyrate dehydrogenase null mutant of Ralstonia eutropha and recombinant Escherichia coli.
abstract: Two systems, one using an (R)-(-)-3-hydroxybutyrate dehydrogenase (BDH) null mutant of Ralstonia eutropha and the other using a recombinant Escherichia coli strain containing a synthetic poly[(R)-(-)-3-hydroxybutyrate] (PHB) operon and an extracellular PHB depolymerase gene, were used for the fermentative production of (R)-(-)-3-hydroxybutyrate (3HB). The concentration of 3HB in the culture supernatant of the mutant R. eutropha system reached about 30 mM after 5 d under anaerobic conditions, although it was about 4-10 mM under aerobic conditions. On the other hand, the 3HB concentration in the culture supernatant of the recombinant E. coli system reached about 70 mM after 4 d, indicating that about 70% of the glucose added was converted to 3HB.
authors.affiliation: Laboratory of Molecular Microbiology, Department of Biological Sciences, Faculty of Science, Kanagawa University, 2946 Tsuchiya, Hiratsuka, Kanagawa 259-1293, Japan.
authors.forename: Mari
authors.initials: M
authors.lastname: Shiraki
authors.forename: Takakazu
authors.initials: T
authors.lastname: Endo
authors.forename: Terumi
authors.initials: T
authors.lastname: Saito
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000378:metabolism*; D020118:Cupriavidus necator / Q000502:physiology*; D004926:Escherichia coli / Q000502:physiology*; D005285:Fermentation / Q000502:physiology; D020868:Gene Silencing / Q000502:physiology; D006884:Hydroxybutyrate Dehydrogenase / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D006884:Hydroxybutyrate Dehydrogenase; D020155:3-Hydroxybutyric Acid