title: Elimination of redundant protein identifications in high throughput proteomics.
abstract: Tandem mass spectrometry followed by data base search is the preferred method for protein identification in high throughput proteomics. However, standard analysis methods give rise to highly redundant lists of proteins with many proteins identified by the same sets of peptides. In essence, this is a list of all proteins that might be present in the sample. Here we present an algorithm that eliminates redundancy and determines the minimum number of proteins needed to explain the peptides observed. We demonstrate that application of the algorithm results in a significantly smaller set of proteins and greatly reduces the number of "shared" peptides.
authors.affiliation: Department of Biomedical Engineering McGill University.
authors.forename: Robert
authors.initials: R
authors.lastname: Kearney
authors.forename: Francois
authors.initials: F
authors.lastname: Blondeau
authors.forename: Peter
authors.initials: P
authors.lastname: McPherson
authors.forename: Alex
authors.initials: A
authors.lastname: Bell
authors.forename: Florence
authors.initials: F
authors.lastname: Servant
authors.forename: Mathieu
authors.initials: M
authors.lastname: Drapeau
authors.forename: Sebastien
authors.initials: S
authors.lastname: de Grandpre
authors.forename: John
authors.initials: J
authors.lastname: Jm Bergeron