title: Dermal condensation formation in the chick embryo: requirement for integrin engagement and subsequent stabilization by a possible notch/integrin interaction.
abstract: During embryonic development, feathers appear first as primordia consisting of an epidermal placode associated with a dermal condensation. When 7-day chick embryo dorsal skin fragments showing three rows of feather primordia are cultured, they undergo a complete reorganization, which involves the down-regulation of morphogenetic genes and dispersal of dermal fibroblasts, leading to the disappearance of primordia. This loss of organisation is followed by de novo differentiation events. We have used this model to study potential factors involved in the formation of dermal condensations. Activation of Integrins by extracellular Manganese or intracellular Calcium prevents the initial disappearance of the dermal condensations. New primordia formation occurs even after inhibition of the Notch pathway albeit with some fusion between primordia. In conclusion, dermal fibroblast migration requires beta1-Integrin whereas the stability of dermal condensations could depend on Notch/Integrin interaction.
authors.affiliation: Centre de Recherche INSERM-Institut Albert Bonniot U823, Ontogenesis and Stem Cell of the Tegument Team, Grenoble, France.
authors.forename: Frederic
authors.initials: F
authors.lastname: Michon
authors.forename: Marie
authors.initials: M
authors.lastname: Charveron
authors.forename: Danielle
authors.initials: D
authors.lastname: Dhouailly
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D002465:Cell Movement / Q000235:genetics / Q000502:physiology; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D020405:Dermis / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D005241:Feathers / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D016023:Integrins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism*
chemical_list: D005353:Fibronectins; D016023:Integrins; D051880:Receptors, Notch