title: Low-temperature-induced Golgi tubules are transient membranes enriched in molecules regulating intra-Golgi transport.
abstract: The incubation of HeLa cells at 15 degrees C induces the formation of Golgi tubules, which contain glycosylation enzymes but neither cargo nor matrix proteins. We now show by immunofluorescence and immunoelectron microscopy that these tubules are enriched in a specific set of SNARE and Rab proteins mediating intra-Golgi transport (Gos28, GS15 and Rab6) but excluded others involved in endoplasmic reticulum-Golgi trafficking (Sec22, membrin, Rab 1 and Rab2). In vivo experiments using cyan fluorescent protein-tagged galactosyltransferase showed that most of these tubules are dynamic transient membranes that grow to the cell periphery but then decrease until disappearing into the perinuclear area. Interestingly, in experiments carried out with cells cultured under physiological conditions, Golgi tubules containing Gos28, GS15, Rab6 and glycosylation enzymes and showing in vivo dynamics identical to that detected in low-temperature-cultured cells were observed. Together, our results support that low-temperature-induced tubules may be representatives of the carriers mediating intra-Golgi recycling of enzymes.
authors.affiliation: Department of Cell Biology, School of Medicine, University of Murcia, 30100 Murcia, Spain.
authors.forename: Emma
authors.initials: E
authors.lastname: Martínez-Alonso
authors.forename: José
authors.initials: J
authors.lastname: Ballesta
authors.forename: José A
authors.initials: JA
authors.lastname: Martínez-Menárguez
mesh_terms: D003080:Cold Temperature; D006056:Golgi Apparatus / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D021381:Protein Transport / Q000502:physiology*; D050600:SNARE Proteins / Q000502:physiology; D020691:rab GTP-Binding Proteins / Q000502:physiology
chemical_list: D050600:SNARE Proteins; D020691:rab GTP-Binding Proteins