title: [X-chromosomal inactivation skewing in blood cells is associated with early development of lung].
abstract: OBJECTIVE
  To observe the relationship between skewed X-chromosomal inactivation (SXCI) and development of lung cancer in females.
  
  
  METHODS
  DNA was isolated from peripheral blood cells from patients with primary lung cancer (n = 148) and control subjects (n =289). Exon 1 of androgen receptor ( AR) gene was amplified, with its products from different alleles resolved on denaturing polyacrylamide gels and visualized by silver staining. The corrected ratio (CR) between products from different AR alleles before and after Hpa II pretreatment was calculated. All statistical tests were two-sided.
  
  
  RESULTS
  With CR> or = 10 adopted as the criterion, SXCI was found more frequently in the younger patients ( C50 years; 7. 9%) than in the controls of the same age group (1. 2% ; P = 0. 046). The SXCI frequency, however, were not significantly different between the old patients ( > 50 years; 4. 5% ) and the controls of the same age group (5. 4% ; P =0. 488). Whether taking CR> or =3 or CR> or =10 as the criteria, the average ages of the patients with SXCI were more than 10 years younger than those without SXCI (P < 0. 05).
  
  
  CONCLUSIONS
  SXCI in blood cells is associated with early development of lung cancer in females.
authors.affiliation: Department of Pathology, Tangdu Hospital, The Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.forename: Qin
authors.initials: Q
authors.lastname: Su
authors.forename: Gui-qiu
authors.initials: GQ
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Gong
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Shao-jun
authors.initials: SJ
authors.lastname: Zhu
authors.forename: He-long
authors.initials: HL
authors.lastname: Zhang
authors.forename: Ying-ming
authors.initials: YM
authors.lastname: Feng
authors.forename: Yu-hai
authors.initials: YH
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000483:Alleles; D041321:Chromosomes, Human, X / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000378:metabolism; D019003:Deoxyribonuclease HpaII / Q000378:metabolism; D005091:Exons; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011944:Receptors, Androgen / Q000235:genetics; D049951:X Chromosome Inactivation
chemical_list: D011944:Receptors, Androgen; D004247:DNA; D019003:Deoxyribonuclease HpaII