title: Endothelin-1 and its receptors ET(A) and ET(B) in drug-induced gingival overgrowth.
abstract: BACKGROUND
  The purpose of this study was to study the expression of endothelin-1 (ET-1) and its receptors ETA and ETB in normal human gingiva and cyclosporin-induced gingival fibroblasts.
  
  
  METHODS
  Gingival samples were collected from eight normal healthy individuals, eight patients with periodontitis, and eight patients with cyclosporin A (CsA)-induced gingival overgrowth. Total RNA was extracted from tissue samples, and reverse transcriptase-polymerase chain reaction was performed for ET-1, ETA, and ETB. ET-1 protein was estimated from the tissues by enzyme-linked immunosorbent assay. The expression of ET-1 and its receptors was also examined in gingival fibroblast cells treated with CsA.
  
  
  RESULTS
  ET-1 mRNA expression was significantly higher in patients with CsA-induced gingival overgrowth (P <0.001) than in patients with periodontitis and the controls. ETA mRNA was expressed more than the ETB in all examined samples. In human gingival fibroblasts, ET-1 expression was increased with CsA incorporation compared to controls (P <0.001).
  
  
  CONCLUSIONS
  These results suggest that CsA can modulate the expression of ET-1 in gingival fibroblasts and CsA-induced gingival overgrowth.
authors.affiliation: Department of Periodontics, Sri Ramachandra Dental College and Hospital, Sri Ramachandra Medical College and Research Institute, Deemed University, Porur, Chennai, Tamilnadu, India.
authors.forename: S
authors.initials: S
authors.lastname: Tamilselvan
authors.forename: Surya Narayana
authors.initials: SN
authors.lastname: Raju
authors.forename: D
authors.initials: D
authors.lastname: Loganathan
authors.forename: S
authors.initials: S
authors.lastname: Kamatchiammal
authors.forename: Georgie
authors.initials: G
authors.lastname: Abraham
authors.forename: R
authors.initials: R
authors.lastname: Suresh
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002478:Cells, Cultured; D016572:Cyclosporine / Q000633:toxicity*; D019332:Endothelin-1 / Q000096:biosynthesis*; D005260:Female; D005347:Fibroblasts / Q000187:drug effects; D019214:Gingival Overgrowth / Q000139:chemically induced* / Q000378:metabolism*; D006801:Humans; D007166:Immunosuppressive Agents / Q000633:toxicity*; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000378:metabolism; D044022:Receptor, Endothelin A / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D019332:Endothelin-1; D007166:Immunosuppressive Agents; D044022:Receptor, Endothelin A; D016572:Cyclosporine