title: [Assessment of occurrence frequency of cotinine and trans-3'-hydroxycotinine in chosen body fluid samples].
abstract: Kind of the main nicotine metabolites, occurrence frequency of trans-3'-hydroxycotinine (HK) and cotinine (K) in mother and foetus serum, amniotic fluid and urine of chosen group of children (10 - 12 years old) determined by planar chromatography with densitometry as well as ratio of (HK)/(K) has been compared. The obtained data allowed to state, that the main nicotine metabolite concentrations (HK and K) were higher in foetus serum in comparison to mother serum. In the case of occurrence both of them ratio of (HK)/(K) was close to 1 in 20% of examined body fluid samples, but it was above this value in significant majority cases. It can be a prove that trans-3'-hydroxycotinine was dominating metabolite of nicotine in these samples and statistically significant more often occur in foetus serum, in the case when cotinine was determined in mother serum.
authors.affiliation: Katedra i Zakład Chemii, Slaska Akademia Medyczna w Katowicach. ktyrpien@wp.pl
authors.forename: Krystyna
authors.initials: K
authors.lastname: Tyrpień
authors.forename: Tomasz
authors.initials: T
authors.lastname: Wielkoszyński
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Kowalska
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Mańka
authors.forename: Cezary
authors.initials: C
authors.lastname: Dobosz
mesh_terms: D000653:Amniotic Fluid / Q000737:chemistry; D001826:Body Fluids / Q000737:chemistry*; D002648:Child; D002855:Chromatography, Thin Layer; D003367:Cotinine / Q000031:analogs & derivatives* / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D009538:Nicotine / Q000378:metabolism*; D011247:Pregnancy; D012907:Smoking / Q000378:metabolism*; D014028:Tobacco Smoke Pollution / Q000032:analysis*
chemical_list: D014028:Tobacco Smoke Pollution; C001381:hydroxycotinine; D009538:Nicotine; D003367:Cotinine