title: A rapid method for the quantification of the enantiomers of Warfarin, Phenprocoumon and Acenocoumarol by two-dimensional-enantioselective liquid chromatography/electrospray tandem mass spectrometry.
abstract: We describe a new fully validated enantioselective LC-MS/MS method for stereospecific quantification of both the racemic forms of Warfarin (WF), Phenprocoumon and Acenocoumarol in human plasma. Measurement specificity was assessed by using different blank donor plasma samples, where no interfering reagent peak appeared at the retention time (RT) of the targeted analytes. Response was linear for all analytes. Typical linear regression coefficients have >0.99. The recoveries ranged from 98% to 118%. Determinations in 10 normal healthy individuals revealed a high reproducibility of RTs. These findings confer to the method suitability for large population studies.
authors.affiliation: Unità di Aterosclerosi e Trombosi, I.R.C.C.S Casa Sollievo della Sofferenza, S. Giovanni Rotondo, Foggia, Italy. g.vecchione@operapadrepio.it
authors.forename: Gennaro
authors.initials: G
authors.lastname: Vecchione
authors.forename: Bruno
authors.initials: B
authors.lastname: Casetta
authors.forename: Michela
authors.initials: M
authors.lastname: Tomaiuolo
authors.forename: Elvira
authors.initials: E
authors.lastname: Grandone
authors.forename: Maurizio
authors.initials: M
authors.lastname: Margaglione
mesh_terms: D000074:Acenocoumarol / Q000032:analysis* / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D010644:Phenprocoumon / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014859:Warfarin / Q000032:analysis* / Q000737:chemistry
chemical_list: D014859:Warfarin; D000074:Acenocoumarol; D010644:Phenprocoumon