title: Effect of elevated dissolved carbon dioxide concentrations on growth of Corynebacterium glutamicum on D-glucose and L-lactate.
abstract: The effect of increased dissolved carbon dioxide concentrations on growth of Corynebacterium glutamicum was studied with continuous turbidostatic cultures. The carbon sources were either l-lactate or d-glucose. To increase the dissolved carbon dioxide concentration the carbon dioxide partial pressure of the inlet gas stream pCO2,IN was increased stepwise from 0.0003 bar (air) up to 0.79 bar, while the oxygen partial pressure of the inlet gas stream was kept constant at 0.21 bar. For each resulting carbon dioxide partial pressure pCO2 the maximum specific growth rate mu(max) was determined from the feed rate resulting from the turbidostatic control. On d-glucose and pCO2 up to 0.26 bar, mu(max) was mostly constant around 0.58 h(-1). Higher pCO2 led to a slight decrease of mu(max). On l-lactate mu(max) increased gradually with increasing carbon dioxide partial pressures from 0.37 h(-1) under aeration with air to a maximum value of 0.47 h(-1) at a pCO2 of 0.26 bar. At very high pCO2 (0.81 bar) mu(max) decreased down to 0.35 h(-1) independent of the carbon source.
authors.affiliation: Biochemical Engineering, RWTH Aachen University, D-52074 Aachen, Germany.
authors.forename: Carsten
authors.initials: C
authors.lastname: Bäumchen
authors.forename: Arnd
authors.initials: A
authors.lastname: Knoll
authors.forename: Bernward
authors.initials: B
authors.lastname: Husemann
authors.forename: Juri
authors.initials: J
authors.lastname: Seletzky
authors.forename: Bernd
authors.initials: B
authors.lastname: Maier
authors.forename: Carsten
authors.initials: C
authors.lastname: Dietrich
authors.forename: Ghassem
authors.initials: G
authors.lastname: Amoabediny
authors.forename: Jochen
authors.initials: J
authors.lastname: Büchs
mesh_terms: D002245:Carbon Dioxide / Q000494:pharmacology*; D048230:Corynebacterium glutamicum / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D010313:Partial Pressure
chemical_list: D002245:Carbon Dioxide; D019344:Lactic Acid; D005947:Glucose