title: Rhythmic cycle of clathrin-coated pit formation at the trans-golgi network in human MDA-MB-435 cells.
abstract: We studied the in vivo dynamics of enhanced green fluorescent protein-tagged clathrin light chain a (GFP-CLCa) at the trans-Golgi network (TGN) in MDA-MB-435 cells. The intensity of fluorescence signals of GFP-CLCa periodically increased and decreased at the TGN approximately every 100 s. This suggests that the formation of clathrin-coated pits occurs synchronously and periodically at the TGN.
authors.affiliation: Laboratory of Molecular Biology and Cell Informatics, Division of Bioscience and Informatics, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, Japan. ssaka@biochem.osakafu-u.ac.jp
authors.forename: Shinji
authors.initials: S
authors.lastname: Sakaushi
authors.forename: Kaori
authors.initials: K
authors.lastname: Senda-Murata
authors.forename: Takashi
authors.initials: T
authors.lastname: Fukada
authors.forename: Shigenori
authors.initials: S
authors.lastname: Oka
authors.forename: Kenji
authors.initials: K
authors.lastname: Sugimoto
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002966:Clathrin / Q000096:biosynthesis*; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D051379:Mice; D008856:Microscopy, Fluorescence; D018715:Microscopy, Video; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D021601:trans-Golgi Network / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D002966:Clathrin; D049452:Green Fluorescent Proteins