[Polymorphism of killer cell immunoglobulin-like receptor 2DL3 gene in Zhejiang Han population].
OBJECTIVE
To analyze the allelic polymorphism of killer cell immunoglobulin-like receptor 2DL3 (KIR2DL3) gene in Zhejiang Han population.


METHODS
Allelic of KIR2DL3 was detected by PCR sequence based typing (PCR-SBT) method. Two specific fragments of KIR2DL3 were amplified using different primers and sequenced for exons 4,5,7,8,9 of KIR2DL3. All the samples would be assigned to KIR2DL3 allele according to the polymorphism site patterns.


RESULTS
Two KIR2DL3 alleles were observed, with KIR2DL3*001 having higher frequency, 0.77.


CONCLUSIONS
The PCR-SBT method is reliable and there are distinctive frequencies of KIR2DL3 alleles in Zhejiang Han population.
Zhu
Fa-ming
FM
Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, Zhejiang, 310006 PR China.
Jiang
Kan
K
Lv
Qin-feng
QF
Zhang
Wei
W
He
Ji
J
Xu
Xian-guo
XG
Yan
Li-xing
LX
D000483:Alleles; D001483:Base Sequence; D002681:China; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D054344:Receptors, KIR2DL3 / Q000235:genetics*; D017422:Sequence Analysis, DNA
D054344:Receptors, KIR2DL3