High formation of superoxide anion and nitric oxide, and matrix metalloproteinases activity in vascular wall of rectal carcinoma vessels.
OBJECTIVE
To study the relationship between the level of generation of reactive oxygen species (ROS) and nitric oxide (NO) and activity of matrix metalloproteinases (MMPs) MMP-2 and MMP-9 in the vessels isolated from rectal tumors and Arteria rectalis superior.


METHODS
EPR at the room temperature and 77 degrees K, Spin Traps technology and zymography in polyacrylamide gels were applied.


RESULTS
In the vessels isolated from rectal tumors and Arteria rectalis superior high levels of ROS, NO and formation of complexes of NO with FeS-proteins at the sites of electron-transporting chain of mitochondria have been detected. High activities of MMP-2 and MMP-9 in vascular wall were also observed. The direct positive correlation between the rate of NO generation and formation of complexes of NO with FeS-proteins as well as between ROS and NO formation and MMPs activities have been revealed.


CONCLUSIONS
Altered oxidative equilibrium in mitochondria of cells in vascular wall promotes formation of cell hypoxia and its autocatalytic potentiation accompanied with activation of MMPs.
Burlaka
A P
AP
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine.
Sidorik
E P
EP
Ganusevich
I I
II
Lestchenko
Yu M
YM
Burlaka
A A
AA
Osinsky
S P
SP
D004578:Electron Spin Resonance Spectroscopy; D004730:Endothelium, Vascular / Q000378:metabolism*; D006801:Humans; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis*; D012004:Rectal Neoplasms / Q000098:blood supply* / Q000378:metabolism*; D019097:Spin Trapping; D013481:Superoxides / Q000378:metabolism*
D013481:Superoxides; D009569:Nitric Oxide; D020782:Matrix Metalloproteinases