title: Inhibition of airway Na+ transport by respiratory syncytial virus.
abstract: In previous studies, we have shown that two major respiratory pathogens, influenza virus and parainfluenza virus, produce acute alterations in ion transport upon contacting the apical membrane of the respiratory epithelium. In the present study, we examine the effects on ion transport by the mouse tracheal epithelium of a third major respiratory pathogen, respiratory syncytial virus (RSV). RSV infections are associated with fluid accumulation in the respiratory tract and cause illnesses that range in severity from rhinitis, sinusitis, otitis media, and bronchitis to bronchiolitis and pneumonia. We find that within minutes of RSV contacting the apical membrane; it inhibits amiloride-sensitive Na+ transport by the epithelium. This effect is mediated by protein kinase C and is reproduced by recombinant viral F (fusion) protein. Since this inhibition is not accompanied by any alteration in the epithelial responses to carbachol or to forskolin plus 3-isobutyl-1-methylxanthine (IBMX), it is not due to a nonspecific toxic action of the virus. The inhibition also appears to require Toll-like receptor 4 and the presence of asialogangliosides in the apical membrane. Since the concentration range over which this inhibition is observed (10(2) to 10(5) PFU/ml) is comparable to the viral concentrations observed in clinical and experimental RSV infections, it seems likely that direct inhibition by the virus of epithelial Na+ transport may contribute to the fluid accumulation that is observed in RSV infections.
authors.affiliation: Institut für Physiologie, Universität Regensburg, Universitätstrasse 31, D-93053 Regensburg, Germany. uqkkunze@mailbox.uq.edu.au
authors.forename: Karl
authors.initials: K
authors.lastname: Kunzelmann
authors.forename: Jane
authors.initials: J
authors.lastname: Sun
authors.forename: Jayesh
authors.initials: J
authors.lastname: Meanger
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: King
authors.forename: David I
authors.initials: DI
authors.lastname: Cook
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry; D053503:Epithelial Sodium Channels / Q000502:physiology*; D006028:Glycosphingolipids / Q000502:physiology; D017136:Ion Transport; D051379:Mice; D011493:Protein Kinase C / Q000502:physiology; D011994:Recombinant Proteins / Q000378:metabolism; D020545:Respiratory Mucosa / Q000378:metabolism* / Q000821:virology*; D012136:Respiratory Syncytial Viruses / Q000502:physiology*; D012964:Sodium / Q000378:metabolism*; D051197:Toll-Like Receptor 4 / Q000502:physiology; D014132:Trachea / Q000378:metabolism / Q000821:virology; D014760:Viral Fusion Proteins / Q000502:physiology
chemical_list: D053503:Epithelial Sodium Channels; D006028:Glycosphingolipids; D011994:Recombinant Proteins; D051197:Toll-Like Receptor 4; D014760:Viral Fusion Proteins; D012964:Sodium; D011493:Protein Kinase C