title: Biological sensing with an on-chip resistive pulse analyzer.
abstract: Resistive pulse sensors (or Coulter counters) detect the conductance change caused by single fluid-borne particles transiting a pore. Their simplicity in design and use, along with their capability for single-molecule sensitivity, make them well-suited to the analysis of biological particles. Here, we use standard methods of micro- and nanolithography to construct resistive-pulse devices that combine microfluidics with electronic sensing. We use the devices to detect single latex colloids, single DNA molecules, and specific antibody/antigen binding. We discuss the advantages of our design, and prospects for future applications.
authors.affiliation: Dept. de Biol., Ecole Normale Superieure, Paris, France.
authors.forename: O A
authors.initials: OA
authors.lastname: Saleh
authors.forename: L L
authors.initials: LL
authors.lastname: Sohn