XPS investigation of DNA binding to zirconium-phosphonate surfaces.
The surface coverage of phosphorylated oligonucleotides immobilized on a zirconium-phosphonate surface was analyzed using X-ray photoelectron spectroscopy (XPS). By quantifying the intensity of the N 1s signal originating from the oligonucleotide and the Zr 3d peak from the metal-phosphonate surface, the surface coverage of the oligonucleotide could be calculated with a modified substrate-overlayer model. We found relatively low surface coverages indicating that once covalently bound via the terminal phosphate the polymer chain further physisorbs to the surface limiting the adsorption of additional molecules.
Lane
Sarah M
SM
Department of Chemistry, University of Florida, Gainesville, FL 32611-7200, USA.
Monot
Julien
J
Petit
Marc
M
Bujoli
Bruno
B
Talham
Daniel R
DR
D004277:DNA, Single-Stranded / Q000737:chemistry*; D063065:Organophosphonates / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods*; D013499:Surface Properties; D014965:X-Rays
D004277:DNA, Single-Stranded; D063065:Organophosphonates