[Serological screening of autoantigens associated with vasculitis in systemic lupus erythematosus].
OBJECTIVE
To screen autoantigens associated with vasculitis in systemic lupus erythematosus (SLE) by serological analysis of recombinant cDNA expression library (SEREX).


METHODS
A cDNA expression library derived from human microvascular endothelial cells (HMVEC) was screened with pooled sera from patients with SLE. Pooled sera were preadsorbed to reduce the background sera activity. The obtained preadsorbed pooled sera of patients were used for immunoscreening. Immunoscreening procedure was done according to the instruction manual provided by the manufacturer (Stratagene).


RESULTS
Eleven distinct positive clones were identified. Bioinformatic analysis revealed these 11 positive clones encoding three different proteins: SSA, SSB and YB-1. As for YB-1, there are hitherto no reports unveiling its association with vasculitis in SLE.


CONCLUSIONS
The association of YB-1 with vasculitis in SLE was found for the first time in this study. SEREX has offered a new strategy to explore the autoantigens related to SLE. By SEREX, we may find out the autoantigen which is helpful to the diagnosis and treatment of SLE, and to the prognostication concerned.
Qin
Wei
W
Department of Nephrology, West China Hospital, Sichuan University, Chengdu 610041, China.
Feng
Sheng-gang
SG
Deng
Hong-xin
HX
Fan
Jun-ming
JM
Zhou
Qin
Q
Wei
Yu-quan
YQ
D000906:Antibodies / Q000097:blood / Q000276:immunology; D001324:Autoantigens / Q000097:blood*; D004268:DNA-Binding Proteins / Q000276:immunology; D042783:Endothelial Cells / Q000276:immunology; D015723:Gene Library; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood* / Q000150:complications*; D055806:Microvessels / Q000473:pathology; D009687:Nuclear Proteins / Q000276:immunology; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D012698:Serologic Tests / Q000379:methods*; D014657:Vasculitis / Q000097:blood* / Q000150:complications*; D051840:Y-Box-Binding Protein 1
D000906:Antibodies; D001324:Autoantigens; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human