[Expression of cathepsin-B and -D in rat's brain after traumatic brain injury].
OBJECTIVE
To study the expression of cathepsin-B and -D in different time point after traumatic brain injury.


METHODS
Traumatic brain injury (TBI) model was established on rats, cathepsin-B and cathepsin-D immunofluorescence staining and confocal microscope analysis were performed. Positive cells were counted by confocal microscope and image analysis techniques were used to determine the morphological changes in each group.


RESULTS
Immunofluorescence staining results showed that cathepsin-B was activated 1 hour after TBI while cathepsin-D was not activated until 12hour after TBI. Both of them got to their peak during 4 to 8days, and kept a high level of activating 32days after TBI. Cathepsin-B and -D positive cells did not merge with caspase-3 positive cells until 6 h after TBI.


CONCLUSIONS
Cathepsin-B and -D could be the diagnostic markers of TBI and can estimating time course of lateral TBI. They blocked caspase-3 activation at the beginning period after TBI and started to promote cell death with caspase-3 6 h after TBI.
Zhang
Yan-bo
YB
Department of Forensic Medicine of Suzhou University, Suzhou 215123, China.
Chen
Xi-ping
XP
Tao
Lu-yang
LY
Qin
Zheng-hong
ZH
Li
Sheng-xing
SX
Yang
Li
L
Yang
Ju
J
Zhang
Yun-ge
YG
Liu
Ran
R
D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D001930:Brain Injuries / Q000378:metabolism* / Q000473:pathology; D053148:Caspase 3 / Q000378:metabolism*; D002401:Cathepsin B / Q000378:metabolism*; D002402:Cathepsin D / Q000378:metabolism*; D004195:Disease Models, Animal; D049429:Forensic Pathology; D006624:Hippocampus / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D008247:Lysosomes; D008297:Male; D009474:Neurons / Q000201:enzymology / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
C505410:Casp3 protein, rat; D053148:Caspase 3; D002401:Cathepsin B; C533916:Ctsb protein, rat; D002402:Cathepsin D; C533924:Ctsd protein, rat