Plant genomic DNA isolation: an art or a science.
Isolating quality DNA from tissues/cells presents a variety of problems in particular when plants are used as the source material. The specific characteristics of plants like the presence of rigid polysaccharide cell wall, pigments, chemical heterogeneity of secondary metabolites found in diverse species of plants, etc., necessitate special consideration and skill during isolation procedure. Until now, numerous protocols have been published for the purpose, but none is found to be universally applicable. Various factors starting from the selection of source material to the concentration of metabolites present in the plant decide the course of the isolation procedure. The present review is an update of various methods used for plant genomic DNA isolation, and it epitomizes the various problems faced and the solutions made to contend with them during DNA isolation from plant cells.
Varma
Astha
A
B. V. Patel Pharmaceutical Education and Research Development Centre, Department of Pharmacognosy and Phytochemistry, Ahmedabad, Gujarat, India.
Padh
Harish
H
Shrivastava
Neeta
N
D001709:Biotechnology / Q000379:methods; D018744:DNA, Plant / Q000737:chemistry / Q000302:isolation & purification*; D005419:Flavonoids / Q000737:chemistry; D018745:Genome, Plant; D010636:Phenols / Q000737:chemistry; D010944:Plants / Q000235:genetics*; D059808:Polyphenols; D011134:Polysaccharides / Q000737:chemistry
D018744:DNA, Plant; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D011134:Polysaccharides