title: Trypanosoma cruzi: exploring the nuclear genome of zymodeme 3 stocks by chromosome size polymorphism.
abstract: Chagas disease is emerging in the Brazilian Amazon. We evaluated the position of eight zymodeme 3 isolates from Amazonian sylvatic vectors and one human case in relation to Trypanosoma cruzi I and II major groups and hybrid strains by chromosome size polymorphism. Nineteen isolates were analyzed by mapping nine coding sequences on chromosomal bands (0.6-3.3Mbp). Numerical analysis was based on the absolute chromosomal size difference index (aCSDI). A dendrogram was obtained applying the minimum evolution criterion and considering the aCSDI values to estimate the branch lengths. The isolates were distributed in four groups. Group A clustered hybrid isolates; Groups B and C, T. cruzi II and T. cruzi I isolates, respectively. Seven Z3 stocks were clustered in Group D, which showed low intra-group diversity and was the most divergent. The proportion of two different-sized homologous chromosomes was determined. Wild vectors harboring Z3 stocks constitute a potential reservoir of human infection in the Amazon.
authors.affiliation: Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Avenida Professor Lineu Prestes, 748, CEP 05508-000, São Paulo, SP, Brazil.
authors.forename: Aurélio
authors.initials: A
authors.lastname: Pedroso
authors.forename: Elisa
authors.initials: E
authors.lastname: Cupolillo
authors.forename: Bianca
authors.initials: B
authors.lastname: Zingales
mesh_terms: D000818:Animals; D014355:Chagas Disease / Q000469:parasitology*; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics / Q000648:ultrastructure; D016000:Cluster Analysis; D005819:Genetic Markers; D014644:Genetic Variation; D018503:Genome, Protozoan / Q000235:genetics*; D006801:Humans; D007303:Insect Vectors / Q000469:parasitology; D011110:Polymorphism, Genetic / Q000235:genetics*; D014225:Triatominae / Q000469:parasitology; D014349:Trypanosoma cruzi / Q000145:classification / Q000235:genetics*
chemical_list: D005819:Genetic Markers