title: Role of phospholipase A2 in the induction of drip loss in porcine muscle.
abstract: The role of phospholipase A2 in the induction of drip loss from pig muscle has been investigated. In samples from porcine M. longissimus dorsi, total PLA2 activity as well as mRNA and protein levels of the group VIA iPLA2 (iPLA2-VIA) increased during the initial 4 h post-mortem period. Morphological studies of porcine muscle showed that at 4 h post-mortem, gaps had formed between muscle fibers and that the sarcolemma membrane borders appeared blurred. At the same time iPLA2-VIA protein levels were increased inside muscle fibers and at the sarcolemma. iPLA2-VIA mRNA abundance in samples from different breeds of pigs with variations in drip loss revealed no clear correlation between drip loss level and iPLA2-VIA expression. Together, these data indicate that during the post-mortem period, iPLA2-VIA expression and activity is increased at the muscle fiber membranes. PLA2 activity may affect membrane permeability and consequently the progression of drip formation in porcine muscle.
authors.affiliation: Institute of Molecular Biology, The August Krogh Building, Universitetsparken 13, DK-2100 Copenhagen Ø, Denmark.
authors.forename: Kristian A
authors.initials: KA
authors.lastname: Poulsen
authors.forename: Jette F
authors.initials: JF
authors.lastname: Young
authors.forename: Peter
authors.initials: P
authors.lastname: Theil
authors.forename: Miriam
authors.initials: M
authors.lastname: Kolko
authors.forename: Niels
authors.initials: N
authors.lastname: Oksbjerg
authors.forename: Ian H
authors.initials: IH
authors.lastname: Lambert
mesh_terms: D000818:Animals; D001834:Body Water / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D018482:Muscle, Skeletal / Q000201:enzymology* / Q000502:physiology*; D010741:Phospholipases A / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D054467:Phospholipases A2; D011180:Postmortem Changes; D012333:RNA, Messenger / Q000032:analysis; D013552:Swine
chemical_list: D012333:RNA, Messenger; D010741:Phospholipases A; D054467:Phospholipases A2