Effect of clomazone herbicide on biochemical and histological aspects of silver catfish (Rhamdia quelen) and recovery pattern.
The effects of the herbicide, clomazone, on acetylcholinesterase (AChE), catalase and TBARS formation in teleost fish (Rhamdia quelen) were studied. The fish were exposed to 0.5 or 1.0 mg L(-1) of clomazone for 12, 24, 48, 96 and 192 h. After 192 h of exposure period, fish were transferred to clean water and kept in the same for 192 h to study the recovery response. Same parameters as that of exposure period were assayed after 96 and 192 h of recovery period. Specific AChE activity was reduced in the brain and muscle after treatments, reaching a maximum inhibition of 47% in the brain and 45% in the muscle after 12h of exposure. Fish exposed to clomazone increased TBARS production in the liver for all exposure periods. The brain presented elevated TBARS levels after 12, 24 and 48 h, but after 96 and 192 h, these levels decreased. The decrease of TBARS levels persisted in brain tissue after 96 h of recovery and returned to the control value after 192 h in clean water. Catalase activity was reduced for all periods of exposure. Histological analysis showed vacuolation in the liver after herbicide exposure. Some of the alterations observed were completely restored after recovery period.
Crestani
Márcia
M
Department of Chemistry, Federal University of Santa Maria, 97105 900 Santa Maria, RS, Brazil.
Menezes
Charlene
C
Glusczak
Lissandra
L
dos Santos Miron
Denise
D
Spanevello
Roselia
R
Silveira
Aron
A
Gonçalves
Fábio Ferreira
FF
Zanella
Renato
R
Loro
Vânia Lúcia
VL
D000110:Acetylcholinesterase / Q000032:analysis; D000383:Agriculture; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D002374:Catalase / Q000378:metabolism; D005260:Female; D005399:Fishes / Q000378:metabolism / Q000502:physiology*; D006540:Herbicides / Q000633:toxicity*; D007555:Isoxazoles / Q000633:toxicity*; D008099:Liver / Q000201:enzymology / Q000473:pathology; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects / Q000201:enzymology; D012275:Oryza; D023303:Oxazolidinones / Q000633:toxicity*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D006540:Herbicides; D007555:Isoxazoles; D023303:Oxazolidinones; D017392:Thiobarbituric Acid Reactive Substances; C095255:clomazone; D002374:Catalase; D000110:Acetylcholinesterase