title: Double-quantum filtered heteronuclear correlation spectroscopy under magic angle spinning.
abstract: We present a simple experimental method to extract the van Vleck second moment of a multiple-spin system under high-resolution condition. The idea is to incorporate a double-quantum (DQ) filter into the pulse sequence of heteronuclear correlation spectroscopy so that a DQ excitation profile can be obtained by measuring a series of 2D spectra. The effects of spinning frequency and proton decoupling are demonstrated on the measurements of two model compounds, viz. hydroxyapatite and brushite. Based on the results obtained for the model compounds, the P-31 homonuclear second moment of the apatite component in rat dentin is characterized. The method is generally suited for the study of bone, enamel and dentin.
authors.affiliation: Department of Chemistry, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei, Taiwan.
authors.forename: Yao-Hung
authors.initials: YH
authors.lastname: Tseng
authors.forename: Yi-Ling
authors.initials: YL
authors.lastname: Tsai
authors.forename: Tim W T
authors.initials: TW
authors.lastname: Tsai
authors.forename: Chun-Pin
authors.initials: CP
authors.lastname: Lin
authors.forename: Shih-Hao
authors.initials: SH
authors.lastname: Huang
authors.forename: Chung-Yuan
authors.initials: CY
authors.lastname: Mou
authors.forename: Jerry C C
authors.initials: JC
authors.lastname: Chan
mesh_terms: D000818:Animals; D003198:Computer Simulation; D003804:Dentin / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D066298:In Vitro Techniques; D007180:Incisor / Q000737:chemistry*; D008954:Models, Biological; D008956:Models, Chemical; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013113:Spin Labels
chemical_list: D013113:Spin Labels; D017886:Durapatite