Femtosecond laser keratome creation of partial thickness donor corneal buttons for lamellar keratoplasty.
OBJECTIVE
To evaluate the thickness and diameter accuracy of the IntraLase femtosecond laser in harvesting corneal discs for lamellar keratoplasty. The stromal bed quality of resected corneas was evaluated by scanning electron microscopy.


METHODS
Two IntraLase units were used to create corneal discs at 225-, 300-, 400-, and 500-microm thickness settings and 7-, 8-, 8.5-, and 9-mm diameter settings (n = 28). Achieved thickness was measured using a digital caliper and achieved diameter was measured using imaging software. Samples were preserved and processed for scanning electron microscopy to observe stromal bed quality.


RESULTS
On both units, the mean deviation from attempted thickness was -9.5 +/- 8.6 microm (range: +6 to -28 microm). All of the obtained corneal discs were circular (horizontal versus vertical diameters, P > .05). The raster pattern produced a smoother stromal bed compared to the spiral pattern.


CONCLUSIONS
The IntraLase femtosecond laser keratome provides depth and diameter accuracy even at increased depth settings. Stromal bed was smooth with the raster pattern and can further be smoothed by excimer phototherapeutic keratectomy treatment. Femtosecond laser lamellar keratoplasty is a promising procedure and clinical data are needed to determine effectiveness.
Sarayba
Melvin A
MA
IntraLase Corp, Irvine, USA. msarayba@intralase.com
Maguen
Ezra
E
Salz
James
J
Rabinowitz
Yaron
Y
Ignacio
Teresa S
TS
D003315:Cornea / Q000166:cytology* / Q000601:surgery; D003319:Corneal Stroma / Q000648:ultrastructure; D016039:Corneal Transplantation / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D006801:Humans; D008855:Microscopy, Electron, Scanning; D013524:Surgical Flaps / Q000592:standards*; D014019:Tissue Donors