Analysis of CD8+ T cell-mediated anti-viral responses in mice with targeted deletions of the M1 or M5 muscarinic cholinergic receptors.
A number of studies have demonstrated that non-neuronal acetylcholine can play a role in the regulation of T cell function. Recently, we reported that CD8(+) T cells, from mice with a targeted deletion of the M(1) muscarinic receptor, had a defect in differentiating into cytolytic T lymphocytes when stimulated in vitro. In the current report, we analyze the in vivo function of CD8(+) T cells from mice with targeted deletions of either M(1) or M(5) muscarinic receptors. M(1) or M(5) knockout mice were infected with either lymphocytic choriomeningitis virus or vesicular stomatitis virus. Expansion of anti-viral CD8(+) T cells was monitored by staining with tetramer reagents specific for the immunodominant peptides of the viruses. No defect in expansion of CD8(+) T cells was observed in either M(1) or M(5) knockout mice. The extent to which one can draw a generalized conclusion that M(1) and M(5) are not involved in anti-viral immunity depends upon issues of antigen strength, genetic background, induction of redundant receptors, and the potential for qualitative defects in the expanded CD8(+) T cells.
Vezys
Vaiva
V
Emory Vaccine Center and Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322, USA.
Masopust
David
D
Desmarets
Maxime
M
Wess
Jürgen
J
Zimring
James C
JC
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000821:virology; D005260:Female; D005434:Flow Cytometry; D017353:Gene Deletion; D008217:Lymphocytic choriomeningitis virus / Q000254:growth & development / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D043643:Receptor, Muscarinic M1 / Q000235:genetics / Q000502:physiology*; D043642:Receptor, Muscarinic M5 / Q000235:genetics / Q000502:physiology*; D013997:Time Factors; D014721:Vesicular stomatitis Indiana virus / Q000254:growth & development / Q000276:immunology; D014777:Virus Diseases / Q000276:immunology*
D043643:Receptor, Muscarinic M1; D043642:Receptor, Muscarinic M5