Spore detection in aerobic granules by different dipicolinic acid releasing methods.
This work aimed at developing a procedure for spore quantification. Spore content was determined by analyzing dipicolinic acid (dpa) extracted from aerobic granules by 13 methods. Concentrated HCl was able to release dpa completely. Results showed that dpa constituted 33.7 mg per g SS, meaning that about 337 mg per g SS were spores, not the normal vegetative cells.
Wang
L
L
Environmental Science and Engineering College, Ocean University of China, 266100 Qingdao, China.
Lin
Y M
YM
D001420:Bacteria, Aerobic / Q000145:classification / Q000378:metabolism*; D019149:Bioreactors; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D010848:Picolinic Acids / Q000302:isolation & purification / Q000378:metabolism*; D013171:Spores, Bacterial / Q000378:metabolism* / Q000648:ultrastructure
D010848:Picolinic Acids; C004860:dipicolinic acid