Analytical detection and biological assay of antileukemic drug 5-fluorouracil using gold nanoparticles as probe.
Gold nanoparticles are reported and evaluated as probes for the detection of anticancer drug 5-fluorouracil (5FU). The nature of binding between 5FU and gold nanoparticles via complexation is investigated using ultraviolet visible spectrophotometry, cyclic voltammetry, transmission electron microscopy, fluorescence and Fourier transform infrared (FTIR) spectroscopy. The bound antileukemic drug is fluorescent and the quenching property of gold nanoparticles could be exploited for biological investigations. The 5FU-colloidal gold complex (Au@5FU) is observed to have appreciable antibacterial and antifungal activity against Micrococcus luteus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Aspergillus fumigatus, and Aspergillus niger. The experimental studies suggest that gold nanoparticles have the potential to be used as effective carriers for anticancer drugs.
Selvaraj
Vaithilingam
V
Department of Chemical Engineering, Alagappa College of Technology, Anna University, Chennai 600 025, India. rajselva_77@yahoo.co.in
Alagar
Muthukaruppan
M
D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D000964:Antimetabolites, Antineoplastic / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D001232:Aspergillus fumigatus / Q000187:drug effects / Q000254:growth & development; D001234:Aspergillus niger / Q000187:drug effects / Q000254:growth & development; D001681:Biological Assay / Q000379:methods*; D004337:Drug Carriers; D004563:Electrochemistry / Q000379:methods; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D005472:Fluorouracil / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D017612:Gold Compounds / Q000737:chemistry / Q000494:pharmacology; D007938:Leukemia / Q000188:drug therapy; D053768:Metal Nanoparticles; D016982:Micrococcus luteus / Q000187:drug effects / Q000254:growth & development; D046529:Microscopy, Electron, Transmission; D015336:Molecular Probe Techniques; D015335:Molecular Probes / Q000737:chemistry / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development
D000890:Anti-Infective Agents; D000964:Antimetabolites, Antineoplastic; D004337:Drug Carriers; D017612:Gold Compounds; D015335:Molecular Probes; C038016:gold chloride; D005472:Fluorouracil