Detection of parC mutations in Streptococcus pneumoniae by Real-time PCR and Taqman-MGB probes.
A Real-time PCR assay was developed by using Taqman-MGB probes to screen mutations at codons Ser79 and Asp83 of Streptococcus pneumoniae parC. One hundred and thirty levofloxacin-susceptible and forty-two levofloxacin-resistant clinical strains were assayed. Mutations at codon 79 were found among all the levofloxacin-resistant strains. Mutations at codon 79 or 83 were found in ten levofloxacin-susceptible strains. This procedure is a reliable method for a rapid detection of mutations in the QRDRs of parC gene of S. pneumoniae and could be carried out in a diagnostic laboratory for some high-risk patients or in epidemiological surveys.
Alonso
Rodrigo
R
Departamento de Inmunología, Microbiología y Parasitología, Facultad de Farmacia, Universidad del País Vasco, Paseo de la Universidad, 7 01006-Vitoria-Gasteiz, Spain. rodrigo.alonso@ehu.es
Mateo
Estibaliz
E
Cisterna
Ramón
R
D000890:Anti-Infective Agents / Q000494:pharmacology; D001224:Aspartic Acid / Q000235:genetics / Q000378:metabolism; D003062:Codon; D004252:DNA Mutational Analysis / Q000379:methods*; D027101:DNA Topoisomerase IV / Q000235:genetics*; D024881:Drug Resistance, Bacterial; D064704:Levofloxacin; D009154:Mutation; D015242:Ofloxacin / Q000494:pharmacology; D015345:Oligonucleotide Probes; D011008:Pneumococcal Infections / Q000175:diagnosis; D016133:Polymerase Chain Reaction / Q000379:methods*; D012694:Serine / Q000235:genetics / Q000378:metabolism; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D019914:Taq Polymerase
D000890:Anti-Infective Agents; D003062:Codon; D015345:Oligonucleotide Probes; D001224:Aspartic Acid; D012694:Serine; D064704:Levofloxacin; D015242:Ofloxacin; D019914:Taq Polymerase; D027101:DNA Topoisomerase IV