Fitness cost of staphylococcal cassette chromosome mec in methicillin-resistant Staphylococcus aureus by way of continuous culture.
We examined the effect of introducing type I or IV staphylococcal cassette chromosome mec (SCCmec) elements on the growth yield of Staphylococcus aureus in glucose-limited continuous culture. Type I showed increased glucose consumption and ATP demand per gram of cells synthesized and decreased cell yield compared to those of the parent strain. In contrast, type IV SCCmec elements had no adverse energetic effect.
Lee
Sui Mae
SM
Department of Microbiology and Immunology, Otago School of Medical Sciences, University of Otago, P.O. Box 56, Dunedin, New Zealand.
Ender
Miriam
M
Adhikari
Rajan
R
Smith
John M B
JM
Berger-Bächi
Brigitte
B
Cook
Gregory M
GM
D002876:Chromosomes, Bacterial / Q000235:genetics*; D003470:Culture Media; D005798:Genes, Bacterial; D005947:Glucose / Q000378:metabolism*; D008712:Methicillin / Q000494:pharmacology; D016106:Methicillin Resistance / Q000235:genetics*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics*
D003470:Culture Media; D005947:Glucose; D008712:Methicillin