[In vivo labeling of cells in the ependyma/subventricular zone of adult rats].
OBJECTIVE
To identify the labeled cells in the ependyma/subventricular zone (SVZ) of normal adult rats by DiI injected into the lateral ventricle.


METHODS
Fifty male Sprague-Dawley rats were divided randomly into five groups (10 per group). All of the rats were injected with 10 microL of 2 g/L fluorescence dye DiI into the right lateral ventricle. The five groups of rats were sacrificed at 6 h, 12 h, 24 h, 36 h or 48 h after injection respectively. Hoechst 33258 staining was used to identify nuclei and laser confocal microscopy was used to detect the DiI-labeled cells and to measure the thickness of the tissue with DiI fluorescence in the wall of the left lateral ventricle.


RESULTS
After injection of the DiI into the right lateral ventricle, DiI- Hoechst 33258 double positive cells were found in the ependymal layer of the left lateral ventricular wall at 24 h and in the SVZ at 48 h as well. The thickness of the tissue with DiI fluorescence in the left ependyma/septal subventricular zone (SVZspt) and ependyma/postnatal equivalent of the ganglionic eminences (SVZge) remained unchanged at 12 h and 24 h after DiI injection. The thickness of the tissue with DiI fluorescence in the left ependyma/SVZge was significantly greater than that in the ependyma/SVZspt at all of the time points (P<0.05).


CONCLUSIONS
The ependyma/SVZ cells can be labeled by Dil 24-48 h after injection (10 microL of 2 g/L) into the lateral ventricle.
Zhang
Peng-bo
PB
Environment and Genes Related to Diseases Key Laboratory of Education Ministry, Medical School of Xi'an Jiaotong University, Xi'an 710061, China.
Liu
Yong
Y
Li
Jie
J
Kang
Qian-yan
QY
Tian
Ying-fang
YF
Zhao
Jian-jun
JJ
Shi
Qin-dong
QD
D000818:Animals; D001930:Brain Injuries / Q000473:pathology; D002232:Carbocyanines / Q000008:administration & dosage / Q000378:metabolism; D004805:Ependyma / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D007267:Injections; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling / Q000379:methods*; D013997:Time Factors
C035005:3,3'-dihexadecylindocarbocyanine; D002232:Carbocyanines