Fertility of spermatozoa cryopreserved with 2% acetamide or glycerol through artificial insemination in the Japanese white rabbit.
The rabbit is considered to be a valuable laboratory animal. We compared 2% acetamide and glycerol as cryoprotectants in egg-yolk diluent for ejaculated Japanese white rabbit spermatozoa to improve sperm cryopreservation methods. Fertility through artificial insemination, forward progressive motility and plasma membrane integrity of the post-thaw spermatozoa were examined. The rates of forward progressive motility and plasma membrane integrity of the spermatozoa frozen with acetamide (27.1 +/- 8.3% and 24.5 +/- 6.5%) were significantly (P < 0.05) higher than those of the spermatozoa frozen with glycerol (16.3 +/- 10.9% and 14.3 +/- 7.6%). Though there was no significant difference in the kindling rates, the litter size of females inseminated with spermatozoa frozen with acetamide (6.0 +/- 1.1) were significantly (P < 0.05) higher than those of spermatozoa frozen with glycerol (3.0 +/- 0.4). The results indicate that 2% acetamide has a higher cryoprotective effect than 2% glycerol for sperm cryopreservation in the Japanese white rabbit.
Okuda
Yasushi
Y
Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University, Fuchinobe, Sagamihara, Kanagawa 229-8501, Japan.
Seita
Yasunari
Y
Hisamatsu
Shin
S
Sonoki
Shigenori
S
Shino
Masao
M
Masaoka
Toshio
T
Inomata
Tomo
T
Kamijo
Shin-ichi
S
Kashiwazaki
Naomi
N
D000081:Acetamides; D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D005260:Female; D005298:Fertility; D005990:Glycerol; D007315:Insemination, Artificial; D008297:Male; D011817:Rabbits; D012662:Semen Preservation / Q000379:methods*; D013094:Spermatozoa / Q000502:physiology*
D000081:Acetamides; D003451:Cryoprotective Agents; C030686:acetamide; D005990:Glycerol