[Hairy roots induction of Phellodendron chinense and production of its active constituents].
OBJECTIVE
To introduce the hairy roots of Phellodendron chinense and determine the content of its active constituents.


METHODS
Transformed hairy roots of P. chinense were obtained by the transformation of Agrobacterium rhizogenes A4, R1600, ATCC15834 and R1000.


CONCLUSIONS
It was clearly demonstrated that T-DNA of A. rhizogenes Ri plasmid was integrated into the cells of hairy roots by PCR. The content of berberine hydrochlodride, which was determined by HPLC, was higher in hairy roots than that in the axenic plantet and callus.
Wang
Yue-hua
YH
Department of Biological Engineering, Chengdu University, Chengdu 610106, China.
Gou
Xiao-jun
XJ
Wu
Jie
J
Yan
Wen-zhao
WZ
Ma
Xian-jun
XJ
Yang
Yi
Y
D001599:Berberine / Q000032:analysis / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D046508:Culture Techniques / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics; D032102:Phellodendron / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D010946:Plants, Medicinal / Q000235:genetics / Q000378:metabolism*; D012231:Rhizobium / Q000235:genetics*; D014170:Transformation, Genetic
D004269:DNA, Bacterial; C036483:T-DNA; D001599:Berberine