Immunolocalization of SNARE proteins in both type II and type III cells of rat taste buds.
Double immunohistochemistry of soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) proteins [synaptosomal-associated protein of 25 kDa (SNAP-25), syntaxin and vesicle-associated protein-2 (VAMP-2)], and specific cell markers of taste buds cells [alpha-gustducin and phospholipase Cbeta2 (PLCbeta2) for type II cells; neural cell adhesion molecule (NCAM) for type III cells] was applied to gustatory epithelia of the rat circumvallate papillae. All three SNARE proteins were present in some elongated taste buds cells as well as intra-, peri- and subgemmal nerve fibers. Double immunohisotochemistry revealed that nearly all alpha-gustducin and PLCbeta2 immunoreactive cells expressed SNAP-25, syntaxin, and VAMP-2. A majority of NCAM immunoreactive cells showed immunoreactivity for these SNARE proteins. These results indicate that these synapse-associated proteins (SNAP-25, syntaxin and VAMP-2) are present in both type II cells and type III cells. Moreover, more than 50% of intragemmal cells containing SNARE proteins showed immunoreactivities for alpha-gustducin, PLCbeta2, and NCAM, suggesting the possible presence of transitional cells having histochemical properties of both type II and type III cells.
Ueda
Katsura
K
Department of Oral Anatomy and Developmental Biology, Osaka University Graduate School of Dentistry, Japan.
Ichimori
Yasuo
Y
Okada
Hiroyuki
H
Honma
Shiho
S
Wakisaka
Satoshi
S
D000818:Animals; D048430:Cell Shape / Q000502:physiology; D007527:Isoenzymes / Q000096:biosynthesis; D008297:Male; D009412:Nerve Fibers / Q000502:physiology; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism; D016612:Paraffin Embedding; D054799:Phospholipase C beta; D050765:Qa-SNARE Proteins / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D050600:SNARE Proteins / Q000096:biosynthesis*; D050825:Synaptosomal-Associated Protein 25 / Q000096:biosynthesis / Q000235:genetics; D013650:Taste Buds / Q000166:cytology* / Q000378:metabolism*; D010738:Type C Phospholipases / Q000096:biosynthesis; D050988:Vesicle-Associated Membrane Protein 2 / Q000096:biosynthesis / Q000235:genetics
D007527:Isoenzymes; D019006:Neural Cell Adhesion Molecules; D050765:Qa-SNARE Proteins; D050600:SNARE Proteins; D050825:Synaptosomal-Associated Protein 25; D050988:Vesicle-Associated Membrane Protein 2; D010738:Type C Phospholipases; D054799:Phospholipase C beta