title: Potential of various archae- and eubacterial strains as industrial polyhydroxyalkanoate producers from whey.
abstract: Three different microbial wild-type strains are compared with respect to their potential as industrial scale polyhydroxyalkanoate (PHA) producers from the feed stock whey lactose. The halophilic archaeon Haloferax mediterranei as well as two eubacterial strains (Pseudomonas hydrogenovora and Hydrogenophaga pseudoflava) are investigated. H. mediterranei accumulated 50 wt.-% of poly-3-(hydroxybutyrate-co-8%-hydroxyvalerate) from hydrolyzed whey without addition of 3-hydroxyvalerate (3HV) precursors (specific productivity q(p): 9.1 mg x g(-1) x h(-1)). Using P. hydrogenovora, the final percentage of poly-3-hydroxybutyrate (PHB) amounted to 12 wt.-% (q(p): 2.9 mg x g(-1) x h(-1)). With H. pseudoflava, it was possible to reach 40 wt.-% P-3(HB-co-5%-HV) on non-hydrolyzed whey lactose plus addition of valeric acid as 3HV precursor (q(p): 12.5 mg x g(-1) x h(-1)). A detailed characterization of the isolated biopolyesters and an evaluation with regard to the economic feasibility completes the study.
authors.affiliation: Graz University of Technology, Institute of Biotechnology and Bioprocess Engineering, Petersgasse 12 A-8010 Graz, Austria. martin.koller@tugraz.at
authors.forename: Martin
authors.initials: M
authors.lastname: Koller
authors.forename: Paula
authors.initials: P
authors.lastname: Hesse
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Bona
authors.forename: Christoph
authors.initials: C
authors.lastname: Kutschera
authors.forename: Aid
authors.initials: A
authors.lastname: Atlić
authors.forename: Gerhart
authors.initials: G
authors.lastname: Braunegg
mesh_terms: D000818:Animals; D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D002152:Calorimetry, Differential Scanning; D042621:Comamonadaceae / Q000378:metabolism*; D019623:Haloferax mediterranei / Q000378:metabolism*; D006880:Hydroxy Acids / Q000378:metabolism*; D006885:Hydroxybutyrates; D007700:Kinetics; D008892:Milk / Q000737:chemistry*; D010421:Pentanoic Acids; D011091:Polyesters; D011108:Polymers / Q000378:metabolism*; D011549:Pseudomonas / Q000378:metabolism*; D000094122:Polyhydroxybutyrates
chemical_list: D006880:Hydroxy Acids; D006885:Hydroxybutyrates; D010421:Pentanoic Acids; D011091:Polyesters; D011108:Polymers; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C038780:n-pentanoic acid