title: Candidate genes and quantitative trait loci affecting fruit ascorbic acid content in three tomato populations.
abstract: Fresh fruit and vegetables are a major source of ascorbic acid (vitamin C), an important antioxidant for the human diet and also for plants. Ascorbic acid content in fruit exhibits a quantitative inheritance. Quantitative trait loci (QTL) for ascorbic acid content have been mapped in three tomato populations derived from crosses between cultivated tomato varieties (Solanum lycopersicum accessions) and three related wild species or subspecies. The first population consists of a set of introgression lines derived from Solanum pennellii, each containing a unique fragment of the wild species genome. The second population is an advanced backcross population derived from a cross between a cultivated tomato and a Solanum habrochaites (formerly Lycopersicum hirsutum) accession. The third population is a recombinant inbred line population derived from the cross between a cherry tomato line and a large fruited line. Common regions controlling ascorbic acid content have been identified on chromosomes 2, 8, 9, 10, and 12. In general, the wild alleles increased ascorbic acid content, but some improvement could also be provided by S. lycopersicum. Most QTLs appeared relatively stable over years and in different environments. Mapping of candidate genes involved in the metabolism of ascorbic acid has revealed a few colocations between genes and QTLs, notably in the case of a monodehydroascorbate reductase gene and a QTL present in two of the populations on chromosome 9 (bin 9-D), and a previously mapped GDP-mannose epimerase and a QTL on chromosome 9 (bin 9-J).
authors.affiliation: Institut National de la Recherche Agronomique, UR1052, Unité de génétique et amélioration des fruits et légumes, Domaine St. Maurice BP94, 84143 Montfavet, France. stevens@avignon.inra.fr
authors.forename: Rebecca
authors.initials: R
authors.lastname: Stevens
authors.forename: Michel
authors.initials: M
authors.lastname: Buret
authors.forename: Philippe
authors.initials: P
authors.lastname: Duffé
authors.forename: Cécile
authors.initials: C
authors.lastname: Garchery
authors.forename: Pierre
authors.initials: P
authors.lastname: Baldet
authors.forename: Christophe
authors.initials: C
authors.lastname: Rothan
authors.forename: Mathilde
authors.initials: M
authors.lastname: Causse
mesh_terms: D001205:Ascorbic Acid / Q000378:metabolism*; D014644:Genetic Variation; D018551:Solanum lycopersicum / Q000235:genetics* / Q000378:metabolism; D040641:Quantitative Trait Loci; D013045:Species Specificity
chemical_list: D001205:Ascorbic Acid