Double-quantum filtered heteronuclear correlation spectroscopy under magic angle spinning.
We present a simple experimental method to extract the van Vleck second moment of a multiple-spin system under high-resolution condition. The idea is to incorporate a double-quantum (DQ) filter into the pulse sequence of heteronuclear correlation spectroscopy so that a DQ excitation profile can be obtained by measuring a series of 2D spectra. The effects of spinning frequency and proton decoupling are demonstrated on the measurements of two model compounds, viz. hydroxyapatite and brushite. Based on the results obtained for the model compounds, the P-31 homonuclear second moment of the apatite component in rat dentin is characterized. The method is generally suited for the study of bone, enamel and dentin.
Tseng
Yao-Hung
YH
Department of Chemistry, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei, Taiwan.
Tsai
Yi-Ling
YL
Tsai
Tim W T
TW
Lin
Chun-Pin
CP
Huang
Shih-Hao
SH
Mou
Chung-Yuan
CY
Chan
Jerry C C
JC
D000818:Animals; D003198:Computer Simulation; D003804:Dentin / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D066298:In Vitro Techniques; D007180:Incisor / Q000737:chemistry*; D008954:Models, Biological; D008956:Models, Chemical; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013113:Spin Labels
D013113:Spin Labels; D017886:Durapatite