[Primary application of spectral karyotyping in leukemia].
OBJECTIVE
To establish a spectral karyotyping (SKY) technique and explore the value of SKY in leukemia research.


METHODS
SKY technique was conducted on 2 samples of peripheral blood from 2 healthy volunteers, then on the samples from 8 patients with leukemia, including chronic myelocytic leukemia (CML) and acute myelocytic leukemia (AML) confirmed by R-banding. In addition, four patients underwent dual fusion-fluorescence in situ hybridization (DF-FISH) to detect the mixed lineage leukemia (MLL), PML/RARa, and BCR/ABL fusion genes. By comparing the results of SKY, R-band karyotyping, and DF-FISH, the stability and reliability of SKY was judged.


RESULTS
All 10 samples were successfully hybridized and karyotyped. The 2 cases of healthy volunteers showed normal karyotypes, thus, a specific SKY technique was successfully established. In the 8 cases of leukemia patients, SKY identified aberrations including 9q-, t (9; 22), t (15; 17) and the complex karyotype 47, XY, +9?ins (1;5) (q23;q23), t (6;7) (q23?; p13), in addition, the SKY technique detected some number abnormalities. The results of SKY confirmed the results of R-band karyotyping and DF-FISH; moreover, the SKY technique provided more accurate karyotypes.


CONCLUSIONS
With high stability, accuracy, and sensitivity, the SKY technique established by this study can be applied in leukemia research.
Guo
Bo
B
General Hospital of People's Liberation Army, Beijing 100853, China.
DA
Wan-ming
WM
Han
Xiao-ping
XP
Sun
Jing-fen
JF
Huang
Wen-rong
WR
Wang
Ke
K
D000328:Adult; D000368:Aged; D002871:Chromosome Banding / Q000379:methods; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007938:Leukemia / Q000175:diagnosis* / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D015514:Oncogene Proteins, Fusion / Q000235:genetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D032681:Spectral Karyotyping / Q000379:methods*; D014178:Translocation, Genetic
D015514:Oncogene Proteins, Fusion