Surfactin from Bacillus subtilis displays anti-proliferative effect via apoptosis induction, cell cycle arrest and survival signaling suppression.
The effect of surfactin on the proliferation of LoVo cells, a human colon carcinoma cell line, was examined. Surfactin strongly blocked the proliferation of LoVo cells by inducing pro-apoptotic activity and arresting the cell cycle, according to several lines of evidence on DNA fragmentation, Annexin V staining, and altered levels of poly (ADP-ribose) polymerase, caspase-3, p21(WAF1/Cip1), p53, CDK2 and cyclin E. The anti-proliferative activity of surfactin was mediated by inhibiting extracellular-related protein kinase and phosphoinositide 3-kinase/Akt activation, as assessed by phosphorylation levels. Therefore, our data suggest that surfactin may have anti-cancer properties as a result of its ability to downregulate the cell cycle and suppress its survival.
Kim
Seo-Young
SY
Department of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
Kim
Joo Young
JY
Kim
Seol-Hee
SH
Bae
Hyun Jin
HJ
Yi
Hwaseon
H
Yoon
Sang Hong
SH
Koo
Bon Sung
BS
Kwon
Moosik
M
Cho
Jae Youl
JY
Lee
Choong-Eun
CE
Hong
Sungyoul
S
D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001412:Bacillus subtilis / Q000737:chemistry*; D053148:Caspase 3 / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D024161:Collagen Type XI / Q000378:metabolism; D053222:Fas Ligand Protein / Q000378:metabolism; D006801:Humans; D055666:Lipopeptides; D010456:Peptides, Cyclic / Q000302:isolation & purification / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D019014:fas Receptor / Q000378:metabolism
D000970:Antineoplastic Agents; C499319:COL11A2 protein, human; D024161:Collagen Type XI; C491901:FAS protein, human; D053222:Fas Ligand Protein; D055666:Lipopeptides; D010456:Peptides, Cyclic; D019014:fas Receptor; C009365:surfactin peptide; D053148:Caspase 3