Ginsenoside Rb1 protects against damage to the spiral ganglion cells after cochlear ischemia.
The effects of transient cochlear ischemia on spiral ganglion cells (SGCs) were studied in Mongolian gerbils. Ischemic insult was induced by occluding the bilateral vertebral arteries of gerbils for 15min. Seven days after ischemia, the percentage of SGCs decreased to 67.5% from the preischemic baseline in the basal turn. Evaluation with immunohistochemical staining showed TUNEL-positive reactions in the SGCs with fragmented nuclei. In addition, we investigated the protective effects of ginsenoside Rb1 (gRb1) against ischemic injury to SGCs. Seven days after ischemia, the auditory brainstem response threshold shift was significantly reduced and the percentage of SGCs decreased to 90.2% from the preischemic baseline in the basal turn in the gRb1-treated group. These findings suggest that gRb1 prevented hearing loss caused by ischemic injury to SGCs in Mongolian gerbils.
Fujita
Kensuke
K
Department of Otolaryngology, Ehime University School of Medicine, Ehime, Japan.
Hakuba
Nobuhiro
N
Hata
Ryuji
R
Morizane
Isao
I
Yoshida
Tadashi
T
Shudou
Masachika
M
Sakanaka
Masahiro
M
Gyo
Kiyofumi
K
D000161:Acoustic Stimulation; D000818:Animals; D002452:Cell Count / Q000379:methods; D015834:Cochlear Diseases / Q000188:drug therapy / Q000473:pathology* / Q000503:physiopathology; D016057:Evoked Potentials, Auditory, Brain Stem / Q000187:drug effects / Q000502:physiology; D005849:Gerbillinae; D036145:Ginsenosides / Q000494:pharmacology* / Q000627:therapeutic use; D020287:In Situ Nick-End Labeling / Q000379:methods; D007511:Ischemia / Q000188:drug therapy / Q000473:pathology* / Q000503:physiopathology; D046529:Microscopy, Electron, Transmission / Q000379:methods; D009474:Neurons / Q000187:drug effects* / Q000648:ultrastructure; D018696:Neuroprotective Agents / Q000494:pharmacology* / Q000627:therapeutic use; D013136:Spiral Ganglion / Q000473:pathology*; D051020:bcl-X Protein / Q000378:metabolism
D036145:Ginsenosides; D018696:Neuroprotective Agents; D051020:bcl-X Protein; C442759:ginsenoside Rb1