title: Nickel-resistant determinant from Leptospirillum ferriphilum.
abstract: Leptospirillum ferriphilum strain UBK03 isolated from a mine in Jiangxi, China, is resistant to Ni(2+) (30 to 40 mM). A four-gene nickel resistance cluster was identified and, when transformed into Escherichia coli, enabled growth in 6 mM nickel. Mutation experiments revealed that the genes ncrA, ncrB, and ncrC could confer nickel resistance in Escherichia coli, whereas the gene ncrY could have a negative effect on nickel resistance.
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing 100081, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Tian
authors.forename: Ningfeng
authors.initials: N
authors.lastname: Wu
authors.forename: Jiang
authors.initials: J
authors.lastname: Li
authors.forename: Yajie
authors.initials: Y
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Guo
authors.forename: Bin
authors.initials: B
authors.lastname: Yao
authors.forename: Yunliu
authors.initials: Y
authors.lastname: Fan
mesh_terms: D000595:Amino Acid Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D024881:Drug Resistance, Bacterial; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000235:genetics; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009532:Nickel / Q000494:pharmacology*
chemical_list: D009532:Nickel