A fundamental dual regulatory role of citrate on the biosyntheses of thuringiensin and poly-beta-hydroxybutyrate in Bacillus thuringiensis YBT-032.
The production of alpha-ketoglutarate, adenine, thuringiensin production rate and thuringiensin yield on glucose consumed increased by 22%, 36%, 40% and 40%, respectively, in presence of 2 g citrate/l. However, citrate decreased pyruvate production, poly-beta-hydroxybutyrate (PHB) production rate and PHB yield by 62%, 31% and 45%, respectively. The activities of pyruvate kinase and glucose-6-phosphate dehydrogenase were 36%-45% lower and 50%-120% higher than those of the control, respectively. The results suggest that citrate regulated the carbon flux to synthesis of adenine present in thuringiensin with a higher efficiency of utilization of glucose by decreasing PHB synthesis.
Wang
Zhi
Z
State Key Laboratory of Agricultural Microbiology, National Engineering Research Center for Microbial Pesticides, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, PR China.
Chen
Shouwen
S
Sun
Ming
M
Yu
Ziniu
Z
D000241:Adenosine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D001413:Bacillus thuringiensis / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D002951:Citrates / Q000494:pharmacology*; D005561:Formates / Q000378:metabolism; D006885:Hydroxybutyrates / Q000378:metabolism*; D007656:Ketoglutaric Acids / Q000378:metabolism; D011091:Polyesters / Q000378:metabolism*; D013400:Sugar Acids; D013997:Time Factors; D000094122:Polyhydroxybutyrates
D000241:Adenosine; D002951:Citrates; D005561:Formates; D006885:Hydroxybutyrates; D007656:Ketoglutaric Acids; D011091:Polyesters; D013400:Sugar Acids; C030544:formic acid; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C068295:thuringiensin