title: Perioperative administration of probiotics: effects on immune response, anastomotic resistance and colonic mucosal trophism.
abstract: OBJECTIVE
  To investigate the effects of perioperative administration of diet containing probiotics on the immune response, anastomotic resistance, and colonic mucosal trophism in rats underwent left-sided colonic anastomosis.
  
  
  METHODS
  Sixty adults Wistar rats were randomized to two groups (A and B) with 30 animals each to receive or not a diet supplemented with probiotics (Streptococcus thermophilus e Lactobacillus helveticus) during pre- and postoperative period. They underwent laparotomy followed by left colon section and immediate anastomosis. Groups of 15 animals were killed either on the 4th (groups A1 and B1) or 7th PO (groups A2 and B2) days. The outcome variables were serum proteins, albumin, globulins and IgA, length and weight of the colon, mucosal weight, DNA content and anastomotic bursting pressure.
  
  
  RESULTS
  Colonic weight was greater in probiotic group compared to controls (1.69+/-0.34 g vs 1.48+/-0.26 g; p<0.05). Total serum proteins and globulin were higher in probiotic animals (proteins = 7.05+/-0.97 g/dl vs 6.57+/-0.66 g/dl e globulins = 5.1+/-0.62 g/dl vs 4.68+/-1.1 g/dl; p<0.05). Serum IgA increased from basal to post-operative days only in the probiotic group (p<0.01).
  
  
  CONCLUSIONS
  Perioperative probiotics enhanced the immune response and colonic weight. Probiotics failed to increase anastomotic resistance.
authors.affiliation: Department of Surgery, Medical School, Federal University of Mato Grosso, Brazil. aguilar@terra.com.br
authors.forename: José Eduardo
authors.initials: JE
authors.lastname: Aguilar-Nascimento
authors.forename: Sávio
authors.initials: S
authors.lastname: Prado
authors.forename: Geazi
authors.initials: G
authors.lastname: Zaffani
authors.forename: Alberto Bicudo
authors.initials: AB
authors.lastname: Salomão
authors.forename: José de Souza
authors.initials: Jde S
authors.lastname: Neves
authors.forename: Diana Borges
authors.initials: DB
authors.lastname: Dock-Nascimento
authors.forename: Paulo Roberto Bezerra de
authors.initials: PR
authors.lastname: Mello
authors.forename: Thelma Suely
authors.initials: TS
authors.lastname: Okay
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D003106:Colon / Q000601:surgery*; D018928:Immunity, Mucosal; D007413:Intestinal Mucosa / Q000276:immunology*; D008297:Male; D019990:Perioperative Care; D011184:Postoperative Period; D019936:Probiotics / Q000008:administration & dosage*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013718:Tensile Strength; D014945:Wound Healing / Q000276:immunology*