In vivo demonstration of FNR dimers in response to lower O(2) availability.
Escherichia coli FNR is an O(2)-sensing transcription factor. In vitro studies indicate that anaerobic iron-sulfur cluster acquisition promotes FNR dimerization. Here, two-hybrid assays show that iron-sulfur cluster-dependent FNR dimers are formed in vivo in response to lower O(2) availability, consistent with the current model of FNR activation.
Jervis
Adrian J
AJ
Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom.
Green
Jeffrey
J
D002876:Chromosomes, Bacterial; D004268:DNA-Binding Proteins / Q000378:metabolism; D019281:Dimerization; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D007506:Iron-Sulfur Proteins / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption; D011993:Recombinant Fusion Proteins / Q000378:metabolism
D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C060704:FNR protein, E coli; D007506:Iron-Sulfur Proteins; D011993:Recombinant Fusion Proteins; D010100:Oxygen