The influence of EDDS and EDTA on the uptake of heavy metals of Cd and Cu from soil with tobacco Nicotiana tabacum.
Phytoextraction, the use of plants to extract contaminants from soils and groundwater, is a promising approach for cleaning up soils contaminated with heavy metals. In order to enhance phytoextraction the use of chelating agents has been proposed. This study aims to assess whether ethylene diamine disuccinate (EDDS), a biodegradable chelator, can be used for enhanced phytoextraction purposed, as an alternative to ethylene diamine tetraacetate (EDTA). EDDS revealed a higher toxicity to tobacco (Nicotiana tabacum) in comparison to EDTA, but no toxicity to microorganisms. The uptake of Cu was increased by the addition of EDTA and EDDS, while no increase was observed in the uptake of Cd. Both chelating agents showed a very low root to shoot translocation capability and the translocation factor was lower than the one of the control. Heavy metals where significantly more phytoavailable than in the control, even after harvesting, resulting in a high heavy metal leaching possibility, probably owing to a low biodegradation rate of EDDS. New seedlings which were transplanted into the EDDS treated pots 7d after the phytoextraction experiment, showed signs of necrosis and chlorosis, which resulted in a significantly lower biomass in comparison to the control. The seedlings on the EDTA treated pots showed no toxicity signs. Contrary to previous opinions the results of this study revealed the chelating agents EDTA and EDDS as unsuitable for enhanced phytoextraction using tobacco.
Evangelou
Michael W H
MW
Institut für Biologie V, RWTH Aachen, Worringerweg 1, 52056 Aachen, Germany. evangelou@bio5.rwth-aachen.de
Bauer
Uwe
U
Ebel
Mathias
M
Schaeffer
Andreas
A
D001673:Biodegradation, Environmental / Q000187:drug effects*; D018533:Biomass; D002104:Cadmium / Q000378:metabolism*; D002614:Chelating Agents / Q000494:pharmacology* / Q000633:toxicity; D003300:Copper / Q000378:metabolism*; D004492:Edetic Acid / Q000494:pharmacology* / Q000633:toxicity; D005029:Ethylenediamines / Q000494:pharmacology* / Q000633:toxicity; D012989:Soil Pollutants / Q000378:metabolism*; D013386:Succinates / Q000494:pharmacology* / Q000633:toxicity; D014026:Nicotiana / Q000187:drug effects / Q000378:metabolism*
D002614:Chelating Agents; D005029:Ethylenediamines; D012989:Soil Pollutants; D013386:Succinates; D002104:Cadmium; C041447:N,N'-ethylenediamine disuccinic acid; D003300:Copper; D004492:Edetic Acid