[Immunohistochemical detection of transcription factors CREB and c-Fos activation in the land snail nervous system induced by pentylenetetrazole].
Phosphorylation of transcription factor CREB and expression of immediate early gene c-fos play a key role in molecular mechanisms of long-term neuronal plasticity in the vertebrate brain. Here, we have defined the procedure of immunohistochemical detection of pCREB and c-Fos in the nervous system of the land snail Helix lucorum (Pulmonata: Stylommatophora) and have shown its activation after the convulsant pentylenetetrazole injection. Baseline pCREB1 and c-Fos levels in the intact snail nervous system appeared to be low. In contrast, injection of pentylenetetrazole (600 mg/kg) produced a rapid induction of CREB phosphorylation and c-Fos expression in a wide range of neurons including a number of identified cells. Double immunofluorescence for pCREB and c-Fos showed that c-Fos was always colocalized with pCREB-immunoreactivity, although the latter had a broader pattern. The results suggest that transcription factors CREB and c-Fos can be used as molecular markers for mapping long-term neuronal plasticity in molluscan nervous system.
Efimova
O I
OI
Ierusalimskiĭ
V N
VN
Anokhin
K V
KV
Balaban
P M
PM
D000818:Animals; D003292:Convulsants / Q000494:pharmacology*; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D006372:Helix, Snails / Q000187:drug effects / Q000378:metabolism*; D007150:Immunohistochemistry; D009420:Nervous System / Q000187:drug effects / Q000378:metabolism*; D010433:Pentylenetetrazole / Q000494:pharmacology*; D010766:Phosphorylation; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*
D003292:Convulsants; D017362:Cyclic AMP Response Element-Binding Protein; D016760:Proto-Oncogene Proteins c-fos; D010433:Pentylenetetrazole