Stereoselective determination of hydroxychloroquine and its metabolites in human urine by liquid-phase microextraction and CE.
Liquid-phase microextraction based on polypropylene hollow fibers and CE were applied for the chiral determination of hydroxychloroquine (HCQ) and its metabolites (desethylchloroquine, DCQ; desethylhydroxychloroquine, DHCQ; bisdesethylchloroquine, BDCQ) in human urine. The analytes were extracted from 3 mL of urine spiked with the internal standard (metoprolol) and alkalinized with 250 muL of 2 M NaOH. The analytes were extracted into 1-octanol impregnated in the pores of the hollow fiber, and into an acid acceptor solution inside the hollow fiber. The electrophoretic separations were carried out in 100 mmol/L Tris buffer (pH adjusted to 9.0 with phosphoric acid) containing 1% w/v S-beta-CD and 30 mg/mL HP-beta-CD with a constant voltage of +18 kV. The method was linear over the concentration range of 10-1000 ng/mL for each HCQ stereoisomer and 21-333 ng/mL for each metabolite stereoisomer. Within-day and between-day assay precision and accuracy for the analytes were studied at three concentration levels for each stereoisomer and were lower than 15%. The developed method was applied for the determination of the cumulative urinary excretion of HCQ, DCQ, and DHCQ after oral administration of rac-HCQ to a health volunteer. The results obtained are in agreement with previous literature data.
de Oliveira
Anderson Rodrigo Moraes
AR
Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil.
Cardoso
Carmen Dickow
CD
Bonato
Pierina Sueli
PS
D018501:Antirheumatic Agents / Q000737:chemistry* / Q000493:pharmacokinetics / Q000652:urine; D019075:Electrophoresis, Capillary; D006801:Humans; D006886:Hydroxychloroquine / Q000737:chemistry* / Q000493:pharmacokinetics / Q000652:urine; D007536:Isomerism
D018501:Antirheumatic Agents; D006886:Hydroxychloroquine