Presenilin 2 is secreted in mouse primary neurons: a release enhanced by apoptosis.
Presenilin 1 (PS1) and presenilin 2 (PS2) are homologous transmembrane proteins genetically associated with Alzheimer disease. As previously reported by our group for PS1, here we demonstrated that, in mouse primary neurons and microglial cells, PS2 C-terminal fragment (CTF) is released by shedding into the extracellular compartment as a soluble form and that this release is 4.07-fold increased during apoptosis. When compared with PS1, PS2 seems to be more susceptible to apoptosis since its secretion is increased 2.8-fold more than PS1. During apoptosis either proteins were colocalized especially within shedded vesicles. The present study suggest an active role for the presenilins CTF on putative target cells.
Ghidoni
Roberta
R
NeuroBioGen Lab-Memory Clinic, IRCCS Centro S. Giovanni di Dio-FBF, AFaR, Brescia, Italy.
Paterlini
Anna
A
Benussi
Luisa
L
Binetti
Giuliano
G
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D051379:Mice; D009474:Neurons / Q000378:metabolism*; D053766:Presenilin-2 / Q000378:metabolism*; D015854:Up-Regulation / Q000502:physiology*
D053766:Presenilin-2; C095101:Psen2 protein, mouse