Novel mitochondria-localizing TEMPO derivative for measurement of cellular oxidative stress in mitochondria.
Neurodegenerative disorders, such as Alzheimer's and Parkinson's diseases, and apoptosis, are thought to be associated with oxidative stress affecting mitochondria. In this study, we designed and synthesized a fluorescein-tagged TEMPO derivative, compound 1, with triphenylphosphino moiety. Synthesized 1 localized in mitochondria and detected oxidative stress in an endotoxic model of a mouse macrophage-like cell line. Compound 1 is therefore a potentially useful probe for evaluating oxidative stress in mitochondria.
Ban
Shizuka
S
Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya, Aichi 467-8603, Japan.
Nakagawa
Hidehiko
H
Suzuki
Takayoshi
T
Miyata
Naoki
N
D000818:Animals; D002460:Cell Line; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D003497:Cyclic N-Oxides / Q000737:chemistry* / Q000494:pharmacology*; D015195:Drug Design; D004578:Electron Spin Resonance Spectroscopy; D051379:Mice; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism*; D008956:Models, Chemical; D008968:Molecular Conformation; D018384:Oxidative Stress; D013997:Time Factors
D003497:Cyclic N-Oxides; C003959:TEMPO