Degrade naphthalene using cells immobilized combining with low-intensity ultrasonic technique.
In this paper, we studied the naphthalene degradation by using Pseudomonas aeruginosa under low-intensity ultrasonic stimulation. In our experiment, the degradation rate of naphthalene was the main parameter. We found that low-intensity ultrasonic could not only promote the growth of immobilized P. aeruginosa, but also could improve the degradation of naphthalene. In this article, 1% naphthalene was added into MM culture medium as imitation wastewater. The effect of low-intensity ultrasonic parameter and gel-globes size were considered. We found the influence was obvious, and the optimum degradation rate was acquired when the parameters of ultrasonic are: frequency, 24 kHz; power, 8 W; ultrasonic time, interval time, 10 s; total time, 10 m and the gel-globes were made by using injector no. 14. The naphthalene degradation rate of immobilized cells with ultrasonic stimulation is 82%, which is 12.9 and 42.2% higher than that of immobilized cells and suspended cells without ultrasonic stimulation, respectively.
Wang
Bochu
B
Key Laboratory of Biomechanics and Tissue Engineering under the State Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China. wangbc2000@126.com
Wang
Qinghong
Q
Liancai
Zhu
Z
Fengwei
Yuan
Y
D001673:Biodegradation, Environmental; D018914:Cells, Immobilized; D003470:Culture Media; D009281:Naphthalenes / Q000737:chemistry / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D014465:Ultrasonics; D014865:Waste Disposal, Fluid
D003470:Culture Media; D009281:Naphthalenes; C031721:naphthalene