title: [Quantification of PPARdelta mRNA by real-time RT-PCR in rectal cancer tissues].
abstract: OBJECTIVE
  To clarify the expression change of PPARdelta gene in human rectal cancer tissues and determine the correlation of PPARdelta expression with the clinical and pathological parameters of rectal cancer.
  
  
  METHODS
  Applying real-time RT-PCR, we quantified PPARdelta mRNA in 86 tissues from excised primary rectal cancers. In each case, accompanying normal mucosa was collected for comparison.
  
  
  RESULTS
  Among the 86 rectal cancer tissues, 48 (55.8%) cases showed PPARdelta overexpression: 39 (81.3%) tumors gave an expression level 1.5 to 5.0 times, 5 (10.4%) tumors 10 to 20 times, and 4 (8.3%) tumors more than 20 times relative to normal mucosa. However, the general level of PPARdelta mRNA in rectal cancer tissues is not statistically different from that in normal mucosa. There was no evidence for the relationships of PPARdelta expression with cell differentiation, pathological categories and Dukes stages.
  
  
  CONCLUSIONS
  The expression of PPARdelta gene in rectal cancers is not statistically different from that in normal mucosa, and it is not correlated with cell differentiation, pathological categories and Dukes stages.
authors.affiliation: Division of Digestive Surgery and Organ-Microcirculation, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Lie
authors.initials: L
authors.lastname: Yang
authors.forename: Yong-yang
authors.initials: YY
authors.lastname: Yu
authors.forename: Zong-guang
authors.initials: ZG
authors.lastname: Zhou
authors.forename: Hong-zhi
authors.initials: HZ
authors.lastname: Luo
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Chao
authors.initials: C
authors.lastname: Tian
authors.forename: Jun
authors.initials: J
authors.lastname: Gu
mesh_terms: D002454:Cell Differentiation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009367:Neoplasm Staging; D047494:PPAR delta / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012004:Rectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D047494:PPAR delta; D012333:RNA, Messenger