title: Unilateral vestibular deafferentation-induced changes in calcium signaling-related molecules in the rat vestibular nuclear complex.
abstract: Inquiries into the neurochemical mechanisms of vestibular compensation, a model of lesion-induced neuronal plasticity, reveal the involvement of both voltage-gated Ca(2+) channels (VGCC) and intracellular Ca(2+) signaling. Indeed, our previous microarray analysis showed an up-regulation of some calcium signaling-related genes such as the alpha2 subunit of L-type calcium channels, calcineurin, and plasma membrane Ca(2+) ATPase 1 (PMCA1) in the ipsilateral vestibular nuclear complex (VNC) following unilateral vestibular deafferentation (UVD). To further elucidate the role of calcium signaling-related molecules in vestibular compensation, we used a quantitative real-time polymerase chain reaction (PCR) method to confirm the microarray results and investigated changes in expression of these molecules at various stages of compensation (6 h to 2 weeks after UVD). We also investigated the changes in gene expression during Bechterew's phenomenon and the effects of a calcineurin inhibitor on vestibular compensation. Real-time PCR showed that genes for the alpha2 subunit of VGCC, PMCA2, and calcineurin were transiently up-regulated 6 h after UVD in ipsilateral VNC. A subsequent UVD, which induced Bechterew's phenomenon, reproduced a complete mirror image of the changes in gene expressions of PMCA2 and calcineurin seen in the initial UVD, while the alpha2 subunit of VGCC gene had a trend to increase in VNC ipsilateral to the second lesion. Pre-treatment by FK506, a calcineurin inhibitor, decelerated the vestibular compensation in a dose-dependent manner. Although it is still uncertain whether these changes in gene expression are causally related to the molecular mechanisms of vestibular compensation, this observation suggests that after increasing the Ca(2+) influx into the ipsilateral VNC neurons via up-regulated VGCC, calcineurin may be involved in their synaptic plasticity. Conversely, an up-regulation of PMCA2, a brain-specific Ca(2+) pump, would increase an efflux of Ca(2+) from those neurons and perhaps prevent cell damage following UVD.
authors.affiliation: Department of Otolaryngology, Osaka University School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Chisako
authors.initials: C
authors.lastname: Masumura
authors.forename: Arata
authors.initials: A
authors.lastname: Horii
authors.forename: Kenji
authors.initials: K
authors.lastname: Mitani
authors.forename: Tadashi
authors.initials: T
authors.lastname: Kitahara
authors.forename: Atsuhiko
authors.initials: A
authors.lastname: Uno
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kubo
mesh_terms: D000344:Afferent Pathways / Q000502:physiology*; D000818:Animals; D019703:Calcineurin / Q000378:metabolism; D065095:Calcineurin Inhibitors; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000235:genetics; D003199:Computer Systems; D003714:Denervation; D015870:Gene Expression; D008297:Male; D009759:Nystagmus, Pathologic / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology; D053499:Plasma Membrane Calcium-Transporting ATPases / Q000235:genetics; D016133:Polymerase Chain Reaction; D020033:Protein Isoforms / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000502:physiology*; D016559:Tacrolimus / Q000494:pharmacology; D014726:Vestibular Nuclei / Q000378:metabolism / Q000502:physiology*
chemical_list: D065095:Calcineurin Inhibitors; D015220:Calcium Channels; D020033:Protein Isoforms; D019703:Calcineurin; D053499:Plasma Membrane Calcium-Transporting ATPases; D002118:Calcium; D016559:Tacrolimus