Regulation of the chitobiose-phosphotransferase system in Vibrio cholerae.
Vibrio cholerae harbours a phosphotransferase system (PTS) enabling the organism to utilise chitosan oligosaccharide, e.g. derived from deacetylated chitin. As shown recently, this utilization system is encoded by the ORFs VC1281-1283 (Meibom et al. in Proc Natl Acad Sci USA, 101:2524-2529, 2004). By using a transcriptional reporter fusion technique, we identified the regulator of the system and characterised gene expression. Furthermore, we found that gene expression of this PTS system is influenced by catabolite regulation and also by an Mlc homologue (VC2007), which in E. coli is a global regulator of sugar metabolism.
Berg
Thorsten
T
Institut für Hygiene und Mikrobiologie, Universität Würzburg, Josef Schneider Str. 2, E1, 97080 Würzburg, Germany.
Schild
Stefan
S
Reidl
Joachim
J
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D048271:Chitosan / Q000378:metabolism; D000242:Cyclic AMP / Q000235:genetics / Q000378:metabolism; D004187:Disaccharides / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D009876:Operon; D010770:Phosphotransferases / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014734:Vibrio cholerae / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D004187:Disaccharides; D029968:Escherichia coli Proteins; C111065:Mlc protein, E coli; D012097:Repressor Proteins; C032438:chitobiose; D048271:Chitosan; D000242:Cyclic AMP; D010770:Phosphotransferases