title: Two vaccine toxicity-related genes Agp and Hpx could prove useful for pertussis vaccine safety control.
abstract: Conventional animal tests such as leukocytosis promoting tests have been used for decades to evaluate toxicity of pertussis vaccine. Here, we examined gene expression in relation to the vaccine toxicity using a DNA microarray. Comparison of conventional animal test data with the DNA microarray-based gene expression data revealed a gene expression pattern highly correlated with leukocytosis in animals. Of 10,490 rat genes analyzed, two genes, alpha1-acid-glycoprotein (Agp) and hemopexin (Hpx), were found up-regulated by the toxin administration in a dose-dependent manner (assayed by a quantitative PCR based on the microarray). Variation of the gene expression was very small amongst the test animals, and the results were highly reproducible. These findings suggest that gene expression analysis of vaccine-treated animals can be used as an accurate and simple method of pertussis vaccine safety assessment.
authors.affiliation: Department of Safety Research on Blood and Biological Products, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan.
authors.forename: Isao
authors.initials: I
authors.lastname: Hamaguchi
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Imai
authors.forename: Haruka
authors.initials: H
authors.lastname: Momose
authors.forename: Mika
authors.initials: M
authors.lastname: Kawamura
authors.forename: Takuo
authors.initials: T
authors.lastname: Mizukami
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kato
authors.forename: Seishiro
authors.initials: S
authors.lastname: Naito
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Maeyama
authors.forename: Atsuko
authors.initials: A
authors.lastname: Masumi
authors.forename: Madoka
authors.initials: M
authors.lastname: Kuramitsu
authors.forename: Kazuya
authors.initials: K
authors.lastname: Takizawa
authors.forename: Masayo
authors.initials: M
authors.lastname: Mochizuki
authors.forename: Masaki
authors.initials: M
authors.lastname: Ochiai
authors.forename: Akihiko
authors.initials: A
authors.lastname: Yamamoto
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Horiuchi
authors.forename: Nobuo
authors.initials: N
authors.lastname: Nomura
authors.forename: Shinya
authors.initials: S
authors.lastname: Watanabe
authors.forename: Kazunari
authors.initials: K
authors.lastname: Yamaguchi
mesh_terms: D000818:Animals; D006466:Hemopexin / Q000235:genetics*; D007964:Leukocytosis / Q000209:etiology; D008099:Liver / Q000378:metabolism; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D009961:Orosomucoid / Q000235:genetics*; D037342:Pertussis Toxin / Q000032:analysis; D010567:Pertussis Vaccine / Q000633:toxicity*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D012449:Safety
chemical_list: D009961:Orosomucoid; D010567:Pertussis Vaccine; D012333:RNA, Messenger; D006466:Hemopexin; D037342:Pertussis Toxin