[Adhesional inhibiton of polyclonal anti-porin I antibody].
To investigate the blockness effects of purified polyclonal anti-porin I antibody on N. gonorrhoeae adherence to genitourinary tract epithelia of BALB/c mouse.



Polyclonal anti GST-PI antibody was generated by immunizing rabbit with GST-PI fusion protein which was constructed and expressed by ourselves. The purified immunoglobulin G was obtained by ammonium sulphate deposition and DEAE cellulose chromatography. Mice model of gonorrhea was established. In order to evaluate the effects of PI-IgG on gonococcus adhesion to vagina mucus, the macroscopic and pathological assessing as well as gonococcus culture was employed after gonococcus challenge on PI-IgG immunized mice.



No pus and pathological inflammation were observed on mice vagina mucus treated with 1 mg/ml PI-IgG 3 hours before gonococcus challenge. Gonococcus could not be detected in the smears and washing solutions from vagina. Pathological inflammation was found in mice treated with anti PI-IgG, in which the concentrations were lower than 1 mg/ml or the treated time was longer than 3 hours prior to gonococcus challenge.



The purified anti PI-IgG can effectively inhibit the adherence and infection of gonococci to genitourinary tract epithelia of BALB/c mice. In addition, the blocking duration of anti PI-IgG is associated with antibody concentration.
Zheng
Lei
L
The Affiliated Childrenos Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China. gauiano@163.com
Ruan
Li-Ming
LM
Cheng
Hao
H
Cenhang
Jian-Ping
JP
Shen
Jian-Gen
JG
Lin
Jie
J
Zhang
Hang
H
Yao
Yun-Bin
YB
Wang
Qi
Q
Chen
Da-Fang
DF
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D004848:Epithelium / Q000187:drug effects / Q000382:microbiology; D005260:Female; D005982:Glutathione Transferase / Q000096:biosynthesis / Q000235:genetics; D006069:Gonorrhea / Q000382:microbiology / Q000517:prevention & control; D051379:Mice; D008807:Mice, Inbred BALB C; D009344:Neisseria gonorrhoeae / Q000187:drug effects / Q000502:physiology; D018272:Porins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D014566:Urogenital System / Q000187:drug effects / Q000382:microbiology
D000911:Antibodies, Monoclonal; D018272:Porins; D011993:Recombinant Fusion Proteins; C057699:porin protein, Neisseria; D005982:Glutathione Transferase