Poor ovarian response to gonadotropin stimulation is associated with low expression of follicle-stimulating hormone receptor in granulosa cells.
OBJECTIVE
To explore the importance of follicle-stimulating hormone receptor (FSHR) in granulosa cells in the ovarian response to gonadotropin stimulation.


METHODS
Prospective study.


METHODS
A women's hospital in China.


METHODS
One hundred infertile women undergoing ovarian stimulation with recombinant follicle-stimulating hormone (rFSH).


METHODS
These women were divided into three groups: poor, moderate, and high responders, according to the number of follicles with diameter >/=14 mm. The FSHR expression at both mRNA and protein levels was determined by either reverse transcription-polymerase chain reaction or Western blot in granulosa cells. E(2) concentrations in serum and FSH levels in serum/follicular fluid (FF) were measured by electrochemiluminescence immunoassay.


METHODS
Relative expression of mRNA and protein of FSHR in granulosa cells, serum E(2), FSH level in serum and FF, and the number of mature follicles.


RESULTS
The expression of FSHR, at both the mRNA and protein levels, was significantly different among the three groups, with the lowest expression in the poor responders. The level of FSHR protein was positively correlated with the peak level of serum E(2) and the number of mature oocytes. FSH levels in FF and the dosage of rFSH used were significantly different among the three groups, with the highest values in the poor responders.


CONCLUSIONS
Different levels of FSHR expression in granulosa cells result in different ovarian response, and lower expression of FSHR may account for poor ovarian response to gonadotropin stimulation, which suggests the critical role of FSHR in the ovarian response to gonadotropin stimulation.
Cai
Jie
J
Department of Reproductive Endocrinology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
Lou
Hang-ying
HY
Dong
Min-yue
MY
Lu
Xiu-e
XE
Zhu
Yi-min
YM
Gao
Hui-juan
HJ
Huang
He-feng
HF
D000328:Adult; D004958:Estradiol / Q000097:blood; D005260:Female; D005307:Fertilization in Vitro; D005640:Follicle Stimulating Hormone / Q000097:blood / Q000627:therapeutic use; D015571:Follicular Fluid / Q000187:drug effects / Q000502:physiology*; D006062:Gonadotropins / Q000502:physiology*; D006107:Granulosa Cells / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007247:Infertility, Female / Q000209:etiology; D010053:Ovary / Q000187:drug effects / Q000503:physiopathology; D010062:Ovulation Induction; D011446:Prospective Studies; D011962:Receptors, FSH / Q000235:genetics*; D011994:Recombinant Proteins / Q000627:therapeutic use; D020133:Reverse Transcriptase Polymerase Chain Reaction
D006062:Gonadotropins; D011962:Receptors, FSH; D011994:Recombinant Proteins; D004958:Estradiol; D005640:Follicle Stimulating Hormone