title: [Study on BEAS-2B cell line exposure to extractable organic matter from ambient fine particles for inflammatory damages].
abstract: OBJECTIVE
  To ascertain the effect of extractable organic matters(EOM) of PM2.5 on inducing inflammation through observing the alteration of IL-8, IL-1p, sICAM-1 that BEAS-2B release. and study the expression of surface molecule CD25 on lymphocyte to find the role of PM2.5 in the process of immunity.
  
  
  METHODS
  To expose BEAS-2B to EOM of PM2.5 and examine the cytokine in culture medium by ELISA, exposing cytokines that BEAS-2B release on human blood lymphocyte and measure the expression of CD25 with flow cytometry.
  
  
  RESULTS
  The control group has a small quantity IL-8,IL-I ,slICAM-1 expressing and increase a little with the prolongation of time. Exposure groups have more notable expression of IL-8, IL-1beta, sICAM-1 than control group, and the difference has statistical significance ( P < 0.01 ).
  
  
  CONCLUSIONS
  BEAS-2B cells exposed to EOM of PM2., could release IL-8, IL-1beta, sICAM-1, which maybe played a role in airway inflammation and airway high responses, and the cytokines released by BEAS-2B cells could advance T lymphocyte proliferate and express molecule CD25, consequently inspired the inflammatory process of airway high responses.
authors.affiliation: School of Public Health, Sun Yat-sen University, Guangzhou 510080, China.
authors.forename: Yi-jian
authors.initials: YJ
authors.lastname: Yang
authors.forename: Hong
authors.initials: H
authors.lastname: Song
mesh_terms: D000393:Air Pollutants; D001980:Bronchi / Q000166:cytology / Q000378:metabolism; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007249:Inflammation / Q000473:pathology; D053645:Interleukin-2 Receptor alpha Subunit / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism
chemical_list: D000393:Air Pollutants; D016207:Cytokines; D053645:Interleukin-2 Receptor alpha Subunit