title: In vivo adaptation of the Wayne model of latent tuberculosis.
abstract: Cultures of Mycobacterium tuberculosis grown under oxygen depletion conditions enter into a state of nonreplicating persistence that may reflect a physiologically latent state. When these cultures were harvested and injected intranasally into mice, no bacteria could be recovered from the lungs for about 3 weeks, but after that evidence of regrowth was observed. Preimmunization of mice with a panel of selected vaccine candidates slowed or prevented this event. This simple model has potential for identifying vaccines targeting latent tuberculosis.
authors.affiliation: Mycobacteria Research Laboratories, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA.
authors.forename: Lisa
authors.initials: L
authors.lastname: Woolhiser
authors.forename: Marcela Henao
authors.initials: MH
authors.lastname: Tamayo
authors.forename: Baolin
authors.initials: B
authors.lastname: Wang
authors.forename: Veronica
authors.initials: V
authors.lastname: Gruppo
authors.forename: John T
authors.initials: JT
authors.lastname: Belisle
authors.forename: Anne J
authors.initials: AJ
authors.lastname: Lenaerts
authors.forename: Randall J
authors.initials: RJ
authors.lastname: Basaraba
authors.forename: Ian M
authors.initials: IM
authors.lastname: Orme
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D008168:Lung / Q000382:microbiology; D051379:Mice; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis / Q000254:growth & development* / Q000378:metabolism / Q000472:pathogenicity* / Q000502:physiology; D010100:Oxygen / Q000494:pharmacology*; D013047:Specific Pathogen-Free Organisms; D014397:Tuberculosis, Pulmonary / Q000382:microbiology / Q000473:pathology*
chemical_list: D010100:Oxygen