Dynamic force spectroscopy of the specific interaction between the PDZ domain and its recognition peptides.
To characterize the molecular basis of specific interactions of PDZ proteins, dynamic force spectroscopy (DFS) for the PDZ protein Tax-interacting protein-1 (TIP-1) and its recognition peptide (PDZ-pep) derived from beta-catenin was performed using an atomic force microscope (AFM), together with measurement of thermodynamic and kinetic parameters using surface plasmon resonance (SPR). The unbinding force of this pair was measured under different conditions of AFM tip-retraction velocity. The relationship between the unbinding force and the logarithmic force-loading rate, that is, the dynamic force spectrum, exhibited two different rate regimes, for each of which the forces increased linearly with the force-loading rate. On the basis of the theoretical treatment of the Bell-Evans model, the positions of two different activation barriers in the reaction coordinate and dissociation rate constants in each barrier were evaluated from slopes and x-intercepts of the two linear regimes (first barrier: 0.04 nm and 1.10 x 10 s(-1); second barrier: 0.21 nm and 2.77 x 10(-2) s(-1), respectively). Although two-step unbinding kinetics between TIP-1 and PDZ-pep was suggested from the DFS analysis, SPR results showed single-step dissociation kinetics with a rate constant of 2.89 x 10(-1) s(-1). Different shapes of the free energy profile of the unbinding process were deduced from each result of DFS and SPR. The reason for such topographic differences in the energy landscape is discussed in relation to the differences in the pathways of forced unbinding and spontaneous dissociation.
Maki
Tei
T
Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency, 4-1-8 Honcho, Saitama 332-0012, Japan.
Kidoaki
Satoru
S
Usui
Kengo
K
Suzuki
Harukazu
H
Ito
Masayoshi
M
Ito
Fuyu
F
Hayashizaki
Yoshihide
Y
Matsuda
Takehisa
T
D002352:Carrier Proteins / Q000737:chemistry*; D002627:Chemistry, Physical / Q000379:methods*; D005898:Glass; D005982:Glutathione Transferase / Q000737:chemistry; D007700:Kinetics; D008024:Ligands; D008780:Methyltransferases; D018625:Microscopy, Atomic Force / Q000379:methods; D010455:Peptides / Q000737:chemistry*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D013057:Spectrum Analysis / Q000379:methods*; D020349:Surface Plasmon Resonance; D013499:Surface Properties; D013816:Thermodynamics
D002352:Carrier Proteins; D008024:Ligands; D010455:Peptides; D008780:Methyltransferases; C503312:tension-induced inhibited protein, mouse; D005982:Glutathione Transferase