Surface imprinted beads for the recognition of human serum albumin.
The synthesis of poly-aminophenylboronic acid (ABPA) imprinted beads for the recognition of the protein human serum albumin (HSA) is reported. In order to create homogeneous recognition sites, covalent immobilisation of the template HSA was exploited. The resulting imprinted beads were selective for HSA. The indirect imprinting factor (IF) calculated from supernatant was 1.6 and the direct IF, evaluated from the protein recovered from the beads, was 1.9. The binding capacity was 1.4 mg/g, which is comparable to commercially available affinity materials. The specificity of the HSA recognition was evaluated with competitive experiments, indicating a molar ratio 4.5/1 of competitor was necessary to displace half of the bound HSA. The recognition and binding of the imprinted beads was also tested with a complex sample, human serum and targeted removal of HSA without a loss of the other protein components was demonstrated. The easy preparation protocol of derivatised beads and a good protein recognition properties make the approach an attractive solution to analytical and bio-analytical problems in the field of biotechnology.
Bonini
Francesca
F
Department of Science and Technology, University of Verona, Strada le Grazie 15, 37134 Verona, Italy.
Piletsky
Sergey
S
Turner
Anthony P F
AP
Speghini
Adolfo
A
Bossi
Alessandra
A
D015374:Biosensing Techniques / Q000295:instrumentation*; D006801:Humans; D008863:Microspheres; D012709:Serum Albumin / Q000032:analysis* / Q000302:isolation & purification
D012709:Serum Albumin