Structural and functional analysis of hypothetical proteins in mouse hippocampus from two-dimensional gel electrophoresis.
Protein profiling in five individual mouse strains showed strain-specific expression of three hypothetical proteins (HPs). As functional and structural assignment of HPs were based on predictions and low identity to known structures, HPs were identified by MALDI-TOF/TOF, and their proposed tentative function was determined by enzyme assays. Three identified HPs were extracted from gels and renatured, and pyridoxal phosphate phosphatase, inorganic pyrophosphate phosphatase, and antioxidant activities were revealed, findings in agreement with functional predictions.
Afjehi-Sadat
Leila
L
Department of Pediatrics, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria.
Yang
Jae-Won
JW
Pollak
Arnold
A
Kim
Dae-Won
DW
Choi
Soo-Young
SY
Lubec
Gert
G
D000595:Amino Acid Sequence; D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D006624:Hippocampus / Q000737:chemistry*; D013058:Mass Spectrometry; D051379:Mice; D008958:Models, Molecular; D008962:Models, Theoretical; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000032:analysis*; D010446:Peptide Fragments / Q000737:chemistry; D011487:Protein Conformation; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D009419:Nerve Tissue Proteins; D010446:Peptide Fragments