title: Applications of LC/MS in structure identifications of small molecules and proteins in drug discovery.
abstract: With advancements in ionization methods and instrumentation, liquid chromatography/mass spectrometry (LC/MS) has become a powerful technology for the characterization of small molecules and proteins. This article will illustrate the role of LC/MS analysis in drug discovery process. Examples will be given on high-throughput analysis, structural analysis of trace level impurities in drug substances, identification of metabolites, and characterization of therapeutic protein products for process improvement. Some unique MS techniques will also be discussed to demonstrate their effectiveness in facilitating structural identifications.
authors.affiliation: Schering-Plough Research Institute, 2015 Galloping Hill Road, Kenilworth, NJ 07033, USA.
authors.forename: Guodong
authors.initials: G
authors.lastname: Chen
authors.forename: Birendra N
authors.initials: BN
authors.lastname: Pramanik
authors.forename: Yan-Hui
authors.initials: YH
authors.lastname: Liu
authors.forename: Urooj A
authors.initials: UA
authors.lastname: Mirza
mesh_terms: D001704:Biopolymers / Q000737:chemistry* / Q000627:therapeutic use; D002853:Chromatography, Liquid / Q000379:methods*; D015195:Drug Design; D013058:Mass Spectrometry / Q000379:methods*; D010449:Peptide Mapping / Q000379:methods*; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry* / Q000627:therapeutic use; D020539:Sequence Analysis, Protein / Q000379:methods*
chemical_list: D001704:Biopolymers; D011506:Proteins