High nitrogen removal from wastewater with several new aerobic bacteria isolated from diverse ecosystems.
Three new bacteria HS-03, HS-043 and HS-047 isolated from different ecosystems were found capable of aerobic denitrification. The potential application of these strains in wastewater treatment under aerobic conditions was investigated. These three bacteria all presented high nitrogen removal from wastewater that more than 98% of 10 mmol/L nitrate could be removed in 12-24 h by adding cheap external carbon source and low concentration of iron as well as molybdate. The mechanism at molecular level was analyzed. The success of this aerobic denitrification applied to wastewater treatment may serve as an alternative to enhance the practical nitrogen removal from wastewater. Main biochemical and physiological features of these strains were characterized. The 16S rDNA sequences were compared with the published data in GenBank by using BLAST. The results of phenotype and genotype proved that strain HS-03 and HS-047 belonged to Pseudomonas stutzeri and Pseudomonas pseudoalcaligenes respectively. Strain HS-043 was identified as Delftia acidovorans of which denitrifying activity has not previously been explored.
Ping
Li
L
College of Life Sciences, Central China Normal University, Wuhan 430079, China.
De-Li
Li
L
Nahimana
Liberat
L
Chen
Shu-Li
SL
Yang
Xi
X
Zhao
Li
L
D001483:Base Sequence; D002681:China; D016000:Cluster Analysis; D019295:Computational Biology; D017931:DNA Primers; D042742:Delftia / Q000235:genetics / Q000378:metabolism*; D004783:Environmental Microbiology; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism*; D010802:Phylogeny; D011549:Pseudomonas / Q000235:genetics / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014865:Waste Disposal, Fluid / Q000379:methods*; D018508:Water Purification / Q000379:methods*
D017931:DNA Primers; D012336:RNA, Ribosomal, 16S; D009584:Nitrogen