title: [Study on antibacterial activity of pheromonicin-SP].
abstract: OBJECTIVE
  To evaluate the bactericidal activity of purified Ph-SP via in vitro/in vivo assays.
  
  
  METHODS
  The recombinant plasmid was transformed into E. coli TG1 cells to produce the fusion peptide, Pheromonicin-Streptococcus pneumoniae (Ph-SP), the peptide was purified by CM sepharose ion-exchange column. In vitro/in vivo bactericidal assays were performed to determine the bactericidal activity of Ph-SP against Streptococcus pneumoniae.
  
  
  RESULTS
  Data of in vitro/in vivo assessments demonstrated that Ph-SP had bactericidal effects against Streptococcus pneumoniae, which was approximately 200 times more than that of penicillin G, on a molar basis, and 50 times more than that of vancomycin.
  
  
  CONCLUSIONS
  The Ph-SP may be of value as a novel model of antibiotics against Streptococcus pneumoniae infection.
authors.affiliation: Laboratory of Transplant Engineering and Immunology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Qin
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Dai
authors.forename: Liang
authors.initials: L
authors.lastname: Sun
authors.forename: Xiao-qing
authors.initials: XQ
authors.lastname: Qiu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D005818:Genetic Engineering; D008297:Male; D051379:Mice; D008826:Microbial Sensitivity Tests; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D013045:Species Specificity; D013296:Streptococcus pneumoniae / Q000187:drug effects* / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents; D010455:Peptides