Effects of propofol and ketamine on ATP-induced contraction of the rat trachea.
OBJECTIVE
ATP causes airway smooth-muscle contraction in patients with asthma and chronic obstructive pulmonary disease. Propofol and ketamine attenuate the airway smooth-muscle contraction induced by histamine and acetylcholine. However, it is not clear whether propofol and ketamine affect the ATP-induced airway smooth-muscle contraction.


METHODS
We examined the effects of propofol and ketamine on the ATP-induced contraction and ATP-P(2)-purinoceptor binding.


RESULTS
Propofol attenuated the ATP-induced contraction in a dose-dependent manner, with a 50% inhibitory concentration of 54 +/- 22 microM. Ketamine at 300 microM attenuated the ATP-induced contraction. In the binding study, propofol attenuated the binding of the P(2)-purinoceptor with [(3)H]-ATP in a dose-dependent manner, while ketamine did not attenuate this binding.


CONCLUSIONS
Propofol attenuates ATP-induced contraction through the inhibition of ATP-P(2)-purinoceptor binding.
Yamaguchi
Masakazu
M
Department of Anesthesiology, Nagasaki University School of Medicine, 1-7-1 Sakamoto, Nagasaki, 852-8501, Japan.
Shibata
Osamu
O
Saito
Masataka
M
Yoshimura
Maki
M
Nishioka
Kenji
K
Makita
Tetsuji
T
Sumikawa
Koji
K
D000255:Adenosine Triphosphate / Q000008:administration & dosage*; D000700:Analgesics / Q000494:pharmacology*; D018686:Anesthetics, Intravenous / Q000494:pharmacology*; D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D007649:Ketamine / Q000494:pharmacology*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D015742:Propofol / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D018048:Receptors, Purinergic P2 / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D014132:Trachea / Q000187:drug effects* / Q000378:metabolism
D000700:Analgesics; D018686:Anesthetics, Intravenous; D018048:Receptors, Purinergic P2; D007649:Ketamine; D000255:Adenosine Triphosphate; D015742:Propofol