[Relationship between adiponectin receptor 1 gene -3881T/C variant and glucose metabolism in the Chinese].
OBJECTIVE
To investigate the relationship between adiponectin receptor 1 gene (ADIPOR1) single nucleotide polymorphism (SNP) and glucose metabolism and insulin resistance in the Chinese.


METHODS
The genotypes of -3881T/C of ADIPOR1 were determined through polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) in 664 Chinese in Shanghai. Among them, 370 were subjects with normal glucose tolerance and 294 were newly diagnosed diabetic patients without taking any drug. Phenotype measured were: height, weight to calculate body mass index; systolic blood pressure and diastolic blood pressure; plasma glucose level, serum insulin and C-peptide levels of blood obtained both at 0 and 120 minute during a standard 75-gram glucose oral glucose tolerance test. Insulin resistance and beta cell function were assessed by homeostasis model assessment (HOMA-IR and HOMA-B).


RESULTS
(1) The frequencies of two alleles did not differ between the type 2 diabetic patients and ones with normal glucose tolerance (P is 0.6749). (2) The frequency of C allele is significantly lower in type 2 diabetic patients with insulin resistance compare with those without insulin resistance (P is 0.0121). (3) In type 2 diabetic patients, the C allele carriers had a significantly lower diastolic blood pressure (P is 0.0466) and HOMA-IR (P is 0.0498). (4) In subjects with normal glucose tolerance, the C allele carriers had a significantly lower fasting plasma glucose (P is 0.0140).


CONCLUSIONS
These findings suggest that variant of ADIPOR1 plays a role in glucose metabolism and insulin resistance in the Chinese.
Hu
Cheng
C
Shanghai Clinical Medicine Center for Diabetes, Shanghai Diabetes Institute, Department of Endocrinology and Metabolism, Shanghai Jiaotong University Affiliated No.6 People's Hospital, Shanghai, 200233 PR China.
Jia
Wei-ping
WP
Zhang
Rong
R
Wang
Cong-rong
CR
Fang
Qi-chen
QC
Ma
Xiao-jing
XJ
Lu
Jun-xi
JX
Xu
Jing
J
Xiang
Kun-san
KS
D000368:Aged; D000483:Alleles; D044466:Asian People / Q000235:genetics; D002681:China; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D054419:Receptors, Adiponectin / Q000235:genetics*
C475418:ADIPOR1 protein, human; D054419:Receptors, Adiponectin; D005947:Glucose