Concordant evolution of mitochondrial and nuclear genomes in the wheat pathogen Phaeosphaeria nodorum.
We compared patterns of mitochondrial restriction fragment length polymorphism (RFLP) diversity with patterns of nuclear RFLP diversity to investigate the effects of selection, gene flow, and sexual reproduction on the population genetic structure and evolutionary history of the wheat pathogen Phaeosphaeria nodorum. A total of 315 fungal isolates from Texas, Oregon, and Switzerland were analyzed using seven nuclear RFLP probes that hybridized to discrete loci and purified mitochondrial DNA that hybridized to the entire mtDNA genome. Forty-two different mitochondrial haplotypes and 298 different nuclear haplotypes were detected. The two most frequent mtDNA haplotypes were present in every population and represented 32% of all isolates. High levels of gene flow, low levels of population subdivision, no evidence for either host specificity or cyto-nuclear disequilibrium were inferred from the analysis of both genomes. The concordance in estimates of these population genetic parameters from both genomes suggests that the two genomes experienced similar degrees of migration, genetic drift and selection.
Sommerhalder
Rubik J
RJ
Plant Pathology, Institute of Integrative Biology, ETH Zurich, LFW, Universitaetstrasse 2, CH-8092 Zürich, Switzerland.
McDonald
Bruce A
BA
Zhan
Jiasui
J
D001203:Ascomycota / Q000235:genetics* / Q000302:isolation & purification; D015825:Chromosomes, Fungal / Q000235:genetics*; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D019143:Evolution, Molecular; D051456:Gene Flow; D014644:Genetic Variation; D016681:Genome, Fungal / Q000235:genetics*; D006239:Haplotypes; D009154:Mutation; D009922:Oregon; D012150:Polymorphism, Restriction Fragment Length; D012641:Selection, Genetic; D013557:Switzerland; D013781:Texas; D014908:Triticum / Q000382:microbiology*
D004271:DNA, Fungal; D004272:DNA, Mitochondrial