title: Purification, crystallization and preliminary X-ray diffraction studies of D-tagatose 3-epimerase from Pseudomonas cichorii.
abstract: D-Tagatose 3-epimerase (D-TE) from Pseudomonas cichorii catalyzes the epimerization of various ketohexoses at the C3 position. The epimerization of D-psicose has not been reported with epimerases other than P. cichorii D-TE and D-psicose 3-epimerase from Agrobacterium tumefaciens. Recombinant P. cichorii D-TE has been purified and crystallized. Crystals of P. cichorii D-TE were obtained by the sitting-drop method at room temperature. The crystal belongs to the monoclinic space group P2(1), with unit-cell parameters a = 76.80, b = 94.92, c = 91.73 A, beta = 102.82 degrees . Diffraction data were collected to 2.5 A resolution. The asymmetric unit is expected to contain four molecules.
authors.affiliation: Molecular Structure Research Group, Information Technology Center and Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0793, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Yoshida
authors.forename: Mitsugu
authors.initials: M
authors.lastname: Yamada
authors.forename: Takeyori
authors.initials: T
authors.lastname: Nishitani
authors.forename: Goro
authors.initials: G
authors.lastname: Takada
authors.forename: Ken
authors.initials: K
authors.lastname: Izumori
authors.forename: Shigehiro
authors.initials: S
authors.lastname: Kamitori
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003460:Crystallization; D018360:Crystallography, X-Ray; D005632:Fructose / Q000378:metabolism; D006601:Hexoses / Q000737:chemistry* / Q000378:metabolism; D011549:Pseudomonas / Q000201:enzymology*; D019998:Racemases and Epimerases / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D006601:Hexoses; C003243:psicose; D005632:Fructose; D019998:Racemases and Epimerases; C030192:tagatose