title: Factors related to contraction and mechanical strength of collagen gels seeded with canine endotenon cells.
abstract: Fibroblasts can construct a hydrated collagen lattice to a tissue-like structure that is greatly influenced by initial culture conditions. The purpose of this study was to investigate the effects of cell concentration and collagen concentration on the contraction kinetics and mechanical properties of resultant endotenon-derived fibroblast-seeded collagen lattice. The experiment was designed to evaluate the effect of cell concentration (0, 0.25, 0.5, and 1.0 x10(6) cells/mL) and collagen concentration (0.5, 1.0, 1.5, and 2.0 mg/mL). Collagen lattice contraction was recorded for 42 days, after which time the lattices were mechanically tested. The collagen lattices seeded with higher initial cell concentration had a shorter contraction lag phase (p < 0.01), and exhibited a higher ultimate stress (p < 0.01) and instantaneous and equilibrium modulus (p < 0.01) than those seeded with a lower initial cell concentration. The collagen lattices cultured with a lower initial collagen concentration also had a shorter contraction lag phase, and exhibited greater instantaneous and equilibrium modulus (p < 0.01) than those cultured with higher initial collagen concentration. The collagen lattices of initial 0.5 mg/mL collagen concentration had the highest value of ultimate stress (p < 0.03).
authors.affiliation: Biomechanics Laboratory, Division of Orthopedic Research, Mayo Clinic College of Medicine, Rochester, Minnesota, USA.
authors.forename: Meng-Yi
authors.initials: MY
authors.lastname: Chen
authors.forename: Yulong
authors.initials: Y
authors.lastname: Sun
authors.forename: Chunfeng
authors.initials: C
authors.lastname: Zhao
authors.forename: Mark E
authors.initials: ME
authors.lastname: Zobitz
authors.forename: Kai-Nan
authors.initials: KN
authors.lastname: An
authors.forename: Steven L
authors.initials: SL
authors.lastname: Moran
authors.forename: Peter C
authors.initials: PC
authors.lastname: Amadio
mesh_terms: D000818:Animals; D002452:Cell Count; D018914:Cells, Immobilized; D024042:Collagen Type I / Q000737:chemistry*; D004285:Dogs; D004548:Elasticity; D005347:Fibroblasts / Q000737:chemistry*; D005782:Gels; D007700:Kinetics; D013314:Stress, Mechanical; D023822:Tissue Engineering
chemical_list: D024042:Collagen Type I; D005782:Gels