Intracellular distribution of TiO2-DNA oligonucleotide nanoconjugates directed to nucleolus and mitochondria indicates sequence specificity.
Deoxyribonucleic acid (DNA) oligonucleotides hybridize to matching DNA sequences in cells, as established in the literature, depending on active transcription of the target sequence and local molarity of the oligonucleotide. We investigated the intracellular distribution of nanoconjugates composed of DNA oligonucleotides attached to TiO2 nanoparticles, thus creating a locally increased concentration of the oligonucleotide. Two types of nanoconjugates, with oligonucleotides matching mitochondrial or nucleolar DNA, were specifically retained in mitochondria or nucleoli.
Paunesku
Tatjana
T
Department of Radiation Oncology, Northwestern University, Chicago, Illinois 60611, USA.
Vogt
Stefan
S
Lai
Barry
B
Maser
Jörg
J
Stojićević
Natasa
N
Thurn
Kenneth T
KT
Osipo
Clodia
C
Liu
Hong
H
Legnini
Daniel
D
Wang
Zhou
Z
Lee
Chung
C
Woloschak
Gayle E
GE
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002466:Cell Nucleolus / Q000378:metabolism* / Q000648:ultrastructure; D004272:DNA, Mitochondrial / Q000378:metabolism; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000378:metabolism* / Q000648:ultrastructure; D036103:Nanotechnology; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000378:metabolism*; D016716:PC12 Cells; D051381:Rats; D014025:Titanium / Q000737:chemistry* / Q000378:metabolism*
D004272:DNA, Mitochondrial; D009838:Oligodeoxyribonucleotides; C009495:titanium dioxide; D014025:Titanium