Multichannel recording and stimulation of neuronal cultures grown on microstamped poly-D-lysine.
We report progress toward designable, reproducible, patterned in vitro neuronal cultures. Cell adhesive proteins were directly microcontact printed on microelectrode arrays (MEAs) used as substrates for hippocampal neurons grown in a defined culture medium. The patterned neuronal network circuits were maintained up to one month. The recorded activity was comparable to that of cultures grown on unpatterned uniform surfaces. Time-locked evoked responses were recorded across the networks.
Nam
Yoonkey
Y
Department of Electrical and Computer Engineering, University of Illinois at Urbana--Champaign, Urbana, IL, USA.
Wheeler
Bruce C
BC