Assessment of a DNA vaccine encoding an anchored-glycosylphosphatidylinositol tegumental antigen complexed to protamine sulphate on immunoprotection against murine schistosomiasis.
Protamine sulphate/DNA complexes have been shown to protect DNA from DNase digestion in a lipid system for gene transfer. A DNA-based vaccine complexed to protamine sulphate was used to induce an immune response against Schistosoma mansoni anchored-glycosylphosphatidylinositol tegumental antigen in BALB/c mice. The protection elicited ranged from 33 to 44%. The spectrum of the elicited immune response induced by the vaccine formulation without protamine was characterized by a high level of IgG (IgG1> IgG2a). Protamine sulphate added to the DNA vaccine formulation retained the green fluorescent protein encoding-plasmid longer in muscle and spleen. The experiments in vivo showed that under protamine sulphate effect, the scope of protection remained unchanged, but a modulation in antibody production (IgG1= IgG2a) was observed.
Nascimento
Eduardo J M
EJ
Centro de Pesquisas Ageu Magalhães, Fiocruz, Cidade Universitária, Recife, PE, Brasil.
Amorim
Rosa V
RV
Cavalcanti
André
A
Alves
Veruska F
VF
Nakazawa
Mineo
M
Pereira
Valéria R A
VR
Lucena-Silva
Norma
N
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood / Q000276:immunology*; D000947:Antigens, Helminth / Q000008:administration & dosage / Q000276:immunology*; D005260:Female; D017261:Glycosylphosphatidylinositols / Q000008:administration & dosage / Q000276:immunology*; D006494:Heparin Antagonists / Q000008:administration & dosage / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011479:Protamines / Q000008:administration & dosage / Q000276:immunology*; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000517:prevention & control; D013997:Time Factors; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D017261:Glycosylphosphatidylinositols; D006494:Heparin Antagonists; D007074:Immunoglobulin G; D011479:Protamines; D019444:Vaccines, DNA