title: An approach to the measurement of decay, active fraction of biomass and extracellular polymeric substances: applied to hydrogen-driven denitrification.
abstract: A general method for measurement of active biomass and decay coefficient and Extracellular polymeric substances (EPS) concentration in steady-state biomass was developed. The model was applied to the process of hydrogenotrophic denitrification in order to measure biomass constituents and decay and yield coefficients. It was found that steady-state biomass obtained after operation at 20 day solids retention time (SRT) was composed of 41% active biomass, 25.6% cell debris and 33.4% extracellular polymeric substance. The value of 0.041 d(-1), and 0.27 mg active biomass per mg NO3-N were obtained for decay coefficient and true yield, respectively.
authors.affiliation: Department of Civil Engineering, University of Manitoba, Winnipeg, MB, Canada R3T 5V6.
authors.forename: B
authors.initials: B
authors.lastname: Rezania
authors.forename: J A
authors.initials: JA
authors.lastname: Oleszkiewicz
authors.forename: N
authors.initials: N
authors.lastname: Cicek
mesh_terms: D001673:Biodegradation, Environmental; D018533:Biomass; D006859:Hydrogen / Q000378:metabolism*; D009566:Nitrates / Q000032:analysis; D009584:Nitrogen / Q000302:isolation & purification*; D010100:Oxygen; D011108:Polymers / Q000032:analysis*; D012995:Solubility; D013997:Time Factors
chemical_list: D009566:Nitrates; D011108:Polymers; D006859:Hydrogen; D009584:Nitrogen; D010100:Oxygen