Gr-1+ cells play an essential role in an experimental model of disseminated histoplasmosis.
Recent studies have shown the participation of Gr-1(+) cells in many types of infections; however, the role played by these cells in the immune response to fungal pathogens is controversial. In this study we determined whether Gr-1(+) cells are involved in the protective immune response in systemic Histoplasma capsulatum infection. Depletion of Gr-1(+) cells using the monoclonal antibody (MAb) RB6-8C5 increased histoplasmosis severity and inhibited the subsequent development of a protective immune response. In addition to the increased fungal burden in lungs and spleens, the Th1 response was found to be unbalanced in these mice and the suppression of the cellular immune response seemed to be associated with increased nitric oxide production. Taken together, these results indicate that Gr-1(+) cell depletion at the beginning of infection allows yeast multiplication and increases mice mortality. This study improves the understanding of the role of Gr-1(+) cells on the protective immunity in histoplasmosis.
Sá-Nunes
Anderson
A
Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. do Café s/no, Ribeirão Preto, São Paulo 14040-903, Brazil.
Medeiros
Alexandra I
AI
Sorgi
Carlos A
CA
Soares
Edson G
EG
Maffei
Cláudia M L
CM
Silva
Célio L
CL
Faccioli
Lúcia H
LH
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000276:immunology; D000943:Antigens, Differentiation / Q000276:immunology / Q000378:metabolism*; D001772:Blood Cell Count; D016207:Cytokines / Q000378:metabolism; D006098:Granulocytes / Q000276:immunology* / Q000378:metabolism; D006658:Histoplasma / Q000302:isolation & purification / Q000472:pathogenicity*; D006660:Histoplasmosis / Q000276:immunology* / Q000382:microbiology / Q000401:mortality; D006801:Humans; D008168:Lung / Q000276:immunology / Q000382:microbiology; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000378:metabolism; D013154:Spleen / Q000276:immunology / Q000382:microbiology
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D016207:Cytokines; D009569:Nitric Oxide