Quantitative surface-enhanced resonance Raman scattering of phthalocyanine-labelled oligonucleotides.
The evaluation of phthalocyanine labels for the surface-enhanced resonance Raman scattering (SERRS) detection of oligonucleotides is reported. Three phthalocyanine-labelled oligonucleotides were assessed, each containing a different metal centre. Detection limits for each labelled oligonucleotide were determined using two excitation frequencies where possible. Limits of detection as low as 2.8 x 10(-11) mol. dm(-3) were obtained which are comparable to standard fluorescently labelled probes used in previous SERRS studies. The identification of two phthalocyanine-labelled oligonucleotides without separation was also demonstrated indicating their suitability for multiplexing. This study extends the range of labels suitable for quantitative surface-enhanced resonance Raman scattering with silver nanoparticles and offers more flexibility and choice when considering SERRS for quantitative DNA detection.
Macaskill
A
A
Centre for Molecular Nanometrology, WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow, UK.
Chernonosov
A A
AA
Koval
V V
VV
Lukyanets
E A
EA
Fedorova
O S
OS
Smith
W E
WE
Faulds
K
K
Graham
D
D
D004247:DNA / Q000032:analysis; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry*; D054833:Isoindoles; D008670:Metals / Q000737:chemistry; D015345:Oligonucleotide Probes / Q000032:analysis / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*
D005456:Fluorescent Dyes; D007211:Indoles; D054833:Isoindoles; D008670:Metals; D015345:Oligonucleotide Probes; D004247:DNA; C013647:phthalocyanine