title: The cytosolic ribonuclease inhibitor contributes to intracellular redox homeostasis.
abstract: The hypothesis that the cytosolic RNase inhibitor (cRI) has a role in the protection of the cellular redox homeostasis was investigated testing the effects of oxidants and anti-oxidants on normal, primary endothelial HUVE cells, and malignant HeLa cells, before and after their engineering into cRI-deprived cells. We found that cRI plays an important, possibly a key, physiological role in the protection of cells from redox stress, as demonstrated by decreased GSH levels as well as increased oxidant-induced DNA damage in cRI deprived cells.
authors.affiliation: Department of Structural and Functional Biology, University of Naples Federico II, Complesso Universitario M.S.Angelo, via Cinthia, 80126, Naples, Italy.
authors.forename: Daria Maria
authors.initials: DM
authors.lastname: Monti
authors.forename: Nicola
authors.initials: N
authors.lastname: Montesano Gesualdi
authors.forename: Josef
authors.initials: J
authors.lastname: Matousek
authors.forename: Franca
authors.initials: F
authors.lastname: Esposito
authors.forename: Giuseppe
authors.initials: G
authors.lastname: D'Alessio
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D006367:HeLa Cells; D006706:Homeostasis; D006801:Humans; D010084:Oxidation-Reduction; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D012260:Ribonucleases / Q000037:antagonists & inhibitors*
chemical_list: D000975:Antioxidants; D002352:Carrier Proteins; D004791:Enzyme Inhibitors; D034741:RNA, Small Interfering; C501467:RNH1 protein, human; D012260:Ribonucleases; D005978:Glutathione