Rapid ambient mass spectrometric profiling of intact, untreated bacteria using desorption electrospray ionization.
Desorption electrospray ionization (DESI) allows the rapid acquisition of highly reproducible mass spectra from intact microorganisms under ambient conditions; application of principal component analysis to the data allows sub-species differentiation.
Song
Yishu
Y
Department of Chemistry, 560 Oval Drive, Purdue University, West Lafayette, IN 47906, USA.
Talaty
Nari
N
Tao
W Andy
WA
Pan
Zhengzheng
Z
Cooks
R Graham
RG
D001419:Bacteria / Q000737:chemistry / Q000302:isolation & purification*; D004926:Escherichia coli / Q000737:chemistry / Q000302:isolation & purification; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D010714:Phosphatidylethanolamines / Q000032:analysis; D025341:Principal Component Analysis; D015203:Reproducibility of Results; D012486:Salmonella typhimurium / Q000737:chemistry / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D005231:Fatty Acids, Unsaturated; D010714:Phosphatidylethanolamines; C483858:phosphatidylethanolamine