Evaluation of fluorescently labeled xylopyranosides as probes for proteoglycan biosynthesis.
A new fluorescent analog to the antiproliferative 2-(6-hydroxynaphthyl)-beta-d-xylopyranoside has been synthesized and tested on a T24 cell line. The new analog was efficiently uptaken by the T24 cells but did not initiate priming of GAG chains. The results are similar to other fluorescently labeled analogs and we propose that these compounds are too large and unpolar to efficiently function as GAG-primers.
Johnsson
Richard
R
Organic Chemistry, Lund University, PO Box 124, SE-221 00 Lund, Sweden.
Mani
Katrin
K
Ellervik
Ulf
U
D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000494:pharmacology; D002461:Cell Line, Transformed; D005456:Fluorescent Dyes; D006027:Glycosides / Q000138:chemical synthesis* / Q000494:pharmacology*; D006801:Humans; D008856:Microscopy, Fluorescence; D014176:Protein Biosynthesis / Q000187:drug effects; D011509:Proteoglycans / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D013050:Spectrometry, Fluorescence
D000970:Antineoplastic Agents; D005456:Fluorescent Dyes; D006027:Glycosides; D011509:Proteoglycans; C010807:xylosides