title: Genomic analysis of two porcine encephalomyocarditis virus strains isolated in China.
abstract: Two strains of encephalomyocarditis virus (EMCV), designated BJC3 and HB1, were isolated from an aborted fetus and the heart tissue of a dead piglet that had pericardial fluid, respectively. The complete genomic sequences of the two viruses were determined and analyzed. The size of the genomes of BJC3 and HB1 were 7746 and 7735 nucleotides, respectively, including poly(A) tails. Comparative analysis with the genomic sequences of other EMCV strains showed that BJC3 and HB1 shared higher identity (92.5-99.6%) with BEL-2887A/91, EMCV-R and PV21, but lower identity (83.3-84.6%) with EMC-B, EMC-D and D variants, and only 81.0% with Mengo virus. Two amino acid mutations in the leader protein of the two viruses and one amino acid substitution in VP1 of BJC3 were found in comparison to other EMCV strains Phylogenetic analysis based on the amino acid sequences of the entire ORF revealed that the two Chinese isolates BJC3 and HB1 clustered together with the strains BEL-2887/91, EMCV-R and PV21, which belong to the same genetic subgroup as EMCV-30. Our results provide genomic information for EMCV isolated in China.
authors.affiliation: Key Laboratory of Preventive Veterinary Medicine of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, P.R. China.
authors.forename: G Q
authors.initials: GQ
authors.lastname: Zhang
authors.forename: X N
authors.initials: XN
authors.lastname: Ge
authors.forename: X
authors.initials: X
authors.lastname: Guo
authors.forename: H C
authors.initials: HC
authors.lastname: Yang
mesh_terms: D000034:Abortion, Veterinary / Q000821:virology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018188:Cardiovirus Infections / Q000662:veterinary / Q000821:virology; D002681:China; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D004682:Encephalomyelitis, Enzootic Porcine; D004686:Encephalomyocarditis virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D008969:Molecular Sequence Data; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000662:veterinary / Q000821:virology; D034421:Sus scrofa / Q000821:virology*; D013553:Swine Diseases / Q000821:virology; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D017931:DNA Primers; D004279:DNA, Viral; D015678:Viral Structural Proteins