Nickel-resistant determinant from Leptospirillum ferriphilum.
Leptospirillum ferriphilum strain UBK03 isolated from a mine in Jiangxi, China, is resistant to Ni(2+) (30 to 40 mM). A four-gene nickel resistance cluster was identified and, when transformed into Escherichia coli, enabled growth in 6 mM nickel. Mutation experiments revealed that the genes ncrA, ncrB, and ncrC could confer nickel resistance in Escherichia coli, whereas the gene ncrY could have a negative effect on nickel resistance.
Tian
Jian
J
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing 100081, China.
Wu
Ningfeng
N
Li
Jiang
J
Liu
Yajie
Y
Guo
Jun
J
Yao
Bin
B
Fan
Yunliu
Y
D000595:Amino Acid Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D024881:Drug Resistance, Bacterial; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000235:genetics; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009532:Nickel / Q000494:pharmacology*
D009532:Nickel