title: Evidence of immunostimulating lipoprotein existing in the natural lipoteichoic acid fraction.
abstract: Lipoteichoic acid (LTA) is a cell surface glycoconjugate of gram-positive bacteria and is reported to activate the innate immune system. We previously reported that purified LTA obtained from Enterococcus hirae has no immunostimulating activity, but a subfraction (Eh-AF) in an LTA fraction possesses activity. In this study, we established a mouse monoclonal antibody neutralizing the activity of Eh-AF and investigated its inhibitory effects. Monoclonal antibody (MAbEh1) was established by the immunization of BALB/c mice with Eh-AF, followed by hybridoma screening based on its inhibitory effect for the production of interleukin-6 (IL-6) induced by Eh-AF. MAbEh1 neutralized the production of IL-6 by LTA fraction from not only E. hirae but also Staphylococcus aureus, while it failed to block that of lipopolysaccharide, suggesting that the antibody recognized a common active structure(s) in LTA fractions. Synthetic glycolipids in these LTAs did not induce cytokine production, at least in our system. Interestingly, the antibody was found to inhibit the activity of immunostimulating synthetic lipopeptides, Pam(3)CSK(4) and FSL-1. These results suggest that MAbEh1 neutralizes the activity of lipoprotein-like compounds which is responsible for the activity of the LTA fraction of E. hirae and S. aureus.
authors.affiliation: Department of Nanostructure and Advanced Materials, Kagoshima University, Korimoto 1-21-40, Kagoshima 890-0065, Japan.
authors.forename: Masahito
authors.initials: M
authors.lastname: Hashimoto
authors.forename: Maiko
authors.initials: M
authors.lastname: Furuyashiki
authors.forename: Ryoko
authors.initials: R
authors.lastname: Kaseya
authors.forename: Yuka
authors.initials: Y
authors.lastname: Fukada
authors.forename: Mai
authors.initials: M
authors.lastname: Akimaru
authors.forename: Kazue
authors.initials: K
authors.lastname: Aoyama
authors.forename: Toshiomi
authors.initials: T
authors.lastname: Okuno
authors.forename: Toshihide
authors.initials: T
authors.lastname: Tamura
authors.forename: Teruo
authors.initials: T
authors.lastname: Kirikae
authors.forename: Fumiko
authors.initials: F
authors.lastname: Kirikae
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Eiraku
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Morioka
authors.forename: Yukari
authors.initials: Y
authors.lastname: Fujimoto
authors.forename: Koichi
authors.initials: K
authors.lastname: Fukase
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Takashige
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Moriya
authors.forename: Shoichi
authors.initials: S
authors.lastname: Kusumoto
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Suda
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D045744:Cell Line, Tumor; D016983:Enterococcus / Q000276:immunology*; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology* / Q000302:isolation & purification; D008074:Lipoproteins / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D009500:Neutralization Tests; D013211:Staphylococcus aureus / Q000276:immunology; D013682:Teichoic Acids / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D015850:Interleukin-6; D008070:Lipopolysaccharides; D008074:Lipoproteins; D013682:Teichoic Acids; C009900:lipoteichoic acid