A feasible and quantitative encoding method for microbeads with multicolor quantum dots.
Multicolor encoded beads were achieved by incorporating two color core-shell quantum dots (QDs) (CdSe/ZnS) to commercial polystyrene (PS) beads. By controlling the concentration ratios of the two quantum dots (QDs) in doping solutions, a series of codes with different intensity ratios were obtained. Based on the multiple encoded carboxylic modified polystyrene beads, fluorescent dyes labeled antibodies were distinguished successfully on the beads' surface. It suggests that the encoded beads from this method have the practicability in biological applications and chemical analysis.
Wang
Hai-Qiao
HQ
Key Laboratory of Biomedical Photonics of Ministry of Education-Hubei Bioinformatics and Molecular Imaging Key Laboratory, Huazhong University of Science and Technology, Wuhan, HuBei, 430074 PR China.
Huang
Zhen-Li
ZL
Liu
Tian-Cai
TC
Wang
Jian-Hao
JH
Cao
Yuan-Cheng
YC
Hua
Xiao-Feng
XF
Li
Xiu-Qing
XQ
Zhao
Yuan-Di
YD
D000906:Antibodies / Q000032:analysis; D019187:Cadmium Compounds / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D008856:Microscopy, Fluorescence / Q000379:methods*; D008863:Microspheres; D011137:Polystyrenes / Q000737:chemistry; D045663:Quantum Dots; D018036:Selenium Compounds / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry; D017967:Zinc Compounds / Q000737:chemistry
D000906:Antibodies; D019187:Cadmium Compounds; D005456:Fluorescent Dyes; D011137:Polystyrenes; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; C058667:cadmium selenide; C031238:zinc sulfide