Innate-like gammadelta T cell responses to mycobacterium Bacille Calmette-Guerin using the public V gamma 2 repertoire in Macaca fascicularis.
The V gamma 2 V delta 2 T cell subset responds to Bacille Calmette-Guerin (BCG) immunization in macaques and may be a component of protective immunity against tuberculosis. We characterized the effects of BCG on the V gamma 2 V delta 2 T cell receptor repertoire by comparing the starting population of V gamma 2 chains in cynomolgus macaques with the repertoire found after priming or booster immunization with BCG. The starting repertoire was dominated by public V gamma 2 chain sequences that were found repeatedly among unrelated animals. Primary exposure to BCG triggered expansion of cells expressing public V gamma 2 chains and booster immunization was often associated with contraction of these same subsets. Thus, BCG-reactive V gamma 2 chains were present at high frequency in the repertoire of mycobacteria-naïve macaques and they comprised the major response to primary or booster immunization. Normal selection processes that created the naïve V gamma 2 repertoire in macaques, also encoded the capacity for rapid responses to mycobacteria. The unusual composition of a normal V gamma 2 repertoire helps to explain the powerful gammadelta T cell responses to BCG immunization.
Cairo
Cristiana
C
University of Maryland School of Medicine, Institute of Human Virology, 725 W Lombard St, Baltimore, MD 21201, USA.
Hebbeler
Andrew M
AM
Propp
Nadia
N
Bryant
Joseph L
JL
Colizzi
Vittorio
V
Pauza
C David
CD
D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial / Q000008:administration & dosage; D001500:BCG Vaccine / Q000276:immunology* / Q000494:pharmacology*; D007114:Immunization; D008168:Lung / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology*; D008252:Macaca fascicularis / Q000276:immunology*; D023421:Models, Animal; D008969:Molecular Sequence Data; D009163:Mycobacterium bovis; D016692:Receptors, Antigen, T-Cell, gamma-delta; D016415:Sequence Alignment; D013045:Species Specificity; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D000942:Antigens, Bacterial; D001500:BCG Vaccine; D016692:Receptors, Antigen, T-Cell, gamma-delta