A network-based analysis of polyanion-binding proteins utilizing human protein arrays.
The existence of interactions between many cellular proteins and various polyanionic surfaces within a cell is now well established. The functional role of such interactions, however, remains to be clearly defined. The existence of protein arrays, with a large selection of different kinds of proteins, provides a way to better address a number of aspects of this question. We have therefore investigated the interaction between five cellular polyanions (actin, tubulin, heparin, heparan sulfate, and DNA) and approximately 5,000 human proteins using protein microarrays in an attempt to better understand the functional nature of such interaction(s). We demonstrate that a large number of polyanion-binding proteins exist that contain multiple positively charged regions, are often disordered, are involved in phosphorylation processes, and appear to play a role in protein-protein interaction networks. Considering the crowded nature of cellular interiors, we propose that polyanion-binding proteins interact with a wide variety of polyanionic surfaces in cells in a functionally significant manner.
Salamat-Miller
Nazila
N
Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, Kansas 66047, USA.
Fang
Jianwen
J
Seidel
Christopher W
CW
Assenov
Yassen
Y
Albrecht
Mario
M
Middaugh
C Russell
CR
D006801:Humans; D000071228:Polyelectrolytes; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D040081:Protein Array Analysis; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism
D000071228:Polyelectrolytes; D011108:Polymers; D011506:Proteins