title: Assessing the anti-estrogenic activity of sodium pentachlorophenol in primary cultures of juvenile goldfish (Carassius auratus) hepatocytes using vitellogenin as a biomarker.
abstract: Both pentachlorophenol and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) had been studied widely because of their probable anti-estrogenic activity. Sodium pentachlorophenol (PCP-Na), as a industrial product used in many fields, usually contains a trace of TCDD. The aim of this study was to assess the anti-estrogenic effect of PCP-Na in juvenile goldfish (Carassius auratus) hepatocyte cultures using vitellogenin (VTG) as the biomarker. The ID50 of PCP-Na was investigated and then a series of concentrations (0.001-0.5 microg/ml) of PCP-Na were evaluated to estimate the anti-estrogenic activity. Results showed that PCP-Na was cytotoxic for hepatocytes even at very low concentration < 1.21 microg/ml, and it could not induce VTG at any concentrations tested. Since it failed to stimulate VTG production, the possibility of its anti-estrogenic effect was tested, and a well-known anti-estrogenic compound-tamoxifen was used as positive control. PCP-Na caused a reduction in VTG synthesis in juvenile goldfish (Carassius auratus) hepatocytes at concentrations > 0.1 microg/ml when co-exposure with 1 microg/ml 17beta-estradiol (E2), making its anti-estrogenic activity approximately as potent as tamoxifen. Our results indicate that PCP-Na can act as negative modulators of estrogenic function in juvenile goldfish (Carassius auratus) hepatocytes.
authors.affiliation: China Research Academy of Environmental Sciences, Beijing 100012, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhao
authors.forename: Zheng-Tao
authors.initials: ZT
authors.lastname: Liu
authors.forename: Zhang-Fa
authors.initials: ZF
authors.lastname: Xu
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002681:China; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D004965:Estrogen Antagonists / Q000633:toxicity*; D006054:Goldfish; D022781:Hepatocytes / Q000187:drug effects*; D020128:Inhibitory Concentration 50; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D010416:Pentachlorophenol / Q000633:toxicity*; D014818:Vitellogenesis / Q000187:drug effects*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D004965:Estrogen Antagonists; D014874:Water Pollutants, Chemical; D010416:Pentachlorophenol; D007770:L-Lactate Dehydrogenase