Effects of alimentary lipemia and inflammation on platelet CD40-ligand.
BACKGROUND
In patients with chronic hypercholesterolemia, the CD40-CD40L dyad is upregulated, contributing to the initiation and progression of atherosclerosis. Our aim was to describe the role of postprandial lipemia and inflammatory stimulation on platelet and monocyte activation and CD40-ligand (CD40L) levels.


RESULTS
Before and 2 h after consumption of a defined fatty meal, whole blood samples of 31 healthy subjects were incubated with endotoxin (LPS). CD40-ligand and CD62P expression on platelets, tissue-factor expression on monocytes and platelet-monocyte aggregates were measured with flow cytometry. Soluble CD40-ligand plasma levels were measured with an ELISA. After the meal, serum triglyceride levels increased from 137.6+/-60.5 mg/dl to 201.5+/-75.0 mg/dl. Expression of CD40L and CD62P on platelets and plasma levels of soluble CD40L were significantly decreased. No significant changes after the meal were observed concerning tissue factor expression on monocytes and platelet-monocyte aggregates. Addition of LPS showed no significant effect concerning CD40L or CD62P expression on platelets, whereas the amount of platelet-monocyte aggregates significantly increased under LPS stimulation after the fatty meal.


CONCLUSIONS
Acute alimenatry lipemia leads to a decreased expression of CD40L on platelets and a reduced plasma level of sCD40L, suggesting an increased turnover in the CD40L system.


CONCLUSIONS
Before and after a fatty meal, blood samples of 31 healthy subjects were incubated with LPS. After the meal, expression of CD40L and CD62P on platelets and plasma levels of soluble CD40L were significantly decreased. Addition of LPS showed no effect concerning CD40L or CD62P expression, whereas the amount of platelet-monocyte aggregates significantly increased under LPS stimulation after the fatty meal.
Kälsch
Thorsten
T
1st Department of Medicine, University Hospital Mannheim, Medical Faculty Mannheim, University of Heidelberg, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany.
Elmas
Elif
E
Nguyen
Xuan Duc
XD
Kralev
Stefan
S
Leweling
Hans
H
Klüter
Harald
H
Dempfle
Carl-Erik
CE
Borggrefe
Martin
M
D000328:Adult; D000368:Aged; D001792:Blood Platelets / Q000378:metabolism*; D019013:CD40 Antigens / Q000096:biosynthesis*; D023201:CD40 Ligand / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood; D006949:Hyperlipidemias / Q000378:metabolism*; D007249:Inflammation; D019041:L-Selectin / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D019518:Postprandial Period; D013925:Thromboplastin / Q000378:metabolism
D019013:CD40 Antigens; D008070:Lipopolysaccharides; D019041:L-Selectin; D023201:CD40 Ligand; D013925:Thromboplastin