Estimation of luminal mucin content in rats by measurement of O-linked oligosaccharide chains and direct ELISA.
Changes in the small intestinal mucin contents in rats were evaluated by two methods, viz., a newly established ELISA and a method based on the measurement of O-linked oligosaccharide chains (OSC) as a mucin marker. Significant correlation was observed between the values of ELISA-derived mucins and OSC. The results confirm the usefulness of measurement of OSC as an alternative method for mucin determination.
Tanabe
Hiroki
H
Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Shizuoka, Japan.
Ito
Hiroyuki
H
Sugiyama
Kimio
K
Kiriyama
Shuhachi
S
Morita
Tatsuya
T
D000818:Animals; D015415:Biomarkers; D001835:Body Weight / Q000187:drug effects; D004032:Diet; D004435:Eating; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D007150:Immunohistochemistry; D008297:Male; D009077:Mucins / Q000032:analysis*; D009844:Oligosaccharides / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D012794:Sialic Acids / Q000032:analysis; D015430:Weight Gain / Q000502:physiology
D015415:Biomarkers; D009077:Mucins; D009844:Oligosaccharides; D012794:Sialic Acids