CNTF promotes the survival and differentiation of adult spinal cord-derived oligodendrocyte precursor cells in vitro but fails to promote remyelination in vivo.
Delivery of factors capable of promoting oligodendrocyte precursor cell (OPC) survival and differentiation in vivo is an important therapeutic strategy for a variety of pathologies in which demyelination is a component, including multiple sclerosis and spinal cord injury. Ciliary neurotrophic factor (CNTF) is a neuropoietic cytokine that promotes both survival and maturation of a variety of neuronal and glial cell populations, including oligodendrocytes. Present results suggest that, although CNTF has a potent survival and differentiation promoting effect in vitro on OPCs isolated from the adult spinal cord, CNTF administration in vivo is not sufficient to promote oligodendrocyte remyelination in the glial-depleted environment of unilateral ethidium bromide (EB) lesions.
Talbott
Jason F
JF
MD/PhD Program, University of Louisville, Louisville, KY 40292, USA.
Cao
Qilin
Q
Bertram
James
J
Nkansah
Michael
M
Benton
Richard L
RL
Lavik
Erin
E
Whittemore
Scott R
SR
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D020934:Ciliary Neurotrophic Factor / Q000494:pharmacology*; D004996:Ethidium; D009186:Myelin Sheath / Q000187:drug effects; D009416:Nerve Regeneration / Q000187:drug effects; D009836:Oligodendroglia / Q000166:cytology* / Q000502:physiology*; D051381:Rats; D013116:Spinal Cord / Q000166:cytology* / Q000503:physiopathology; D013118:Spinal Cord Diseases / Q000139:chemically induced / Q000503:physiopathology; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology*
D020934:Ciliary Neurotrophic Factor; D004996:Ethidium