Application of pressure cycling technology to tissue sample preparation for 2-DE.
2-DE is a powerful protein analytical tool whose major strengths include semiglobal quantitation and charge separation of complex protein mixtures, enabling the analysis of differential protein expression, and variable post-translational modification. One of 2-DE's limitations relates to its limited dynamic range and consequently the number of proteins expressed that can be analyzed on a single gel. In an attempt to improve the yield of detectable proteins during sample preparation, we applied a novel extraction technique called pressure cycling technology.
Ringham
Heather
H
Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, IN 46202-2111, USA.
Bell
Richard L
RL
Smejkal
Gary B
GB
Behnke
James
J
Witzmann
Frank A
FA
D053000:Analytic Sample Preparation Methods; D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D006874:Hydrostatic Pressure; D011506:Proteins / Q000032:analysis*; D051381:Rats
D011506:Proteins