Effect of variants in the ovine skeletal-muscle-specific calpain gene on body weight.
The ovine skeletal-muscle-specific calpain gene (p94), which is known also as the n-calpain or calpain 3 gene (CAPN3), was screened with primers. Selection of the PCR primers was based on the ovine cDNA sequence (GenBank accession No. AF087570). After sequence alignment between the ovine and human (AY902237) genes, exon and intron boundaries were determined. Polymorphisms were observed in the intron region for the CAPN31112 and CAPN31213 segments, and the sequences for these segments were submitted to the GenBank (AF309635 and AY102617, respectively). Body weight was recorded at birth, weaning and post-weaning. Calpain 3 genotypes of the CAPN31112 segment were associated with birth weight (P < 0.01), and a dominant gene effect was observed. Breeding group, birth type, and rearing type were significantly associated with weight traits. Allele frequencies were similar in purebred and crossbred animals.
Chung
Hoyoung
H
Animal Genomics and Bioinformatics Division, National Livestock Research Institute, Suwon 441-701 Korea. chung133@rda.go.kr
Choi
Bonghwan
B
Jang
Gilwon
G
Lee
Kyungtai
K
Kim
Hyunju
H
Yoon
Sungho
S
Im
Seokki
S
Davis
Michael
M
Hines
Harold
H
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D001835:Body Weight / Q000235:genetics*; D002154:Calpain / Q000235:genetics*; D004247:DNA / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D014644:Genetic Variation; D009124:Muscle Proteins / Q000235:genetics*; D018482:Muscle, Skeletal / Q000378:metabolism; D018807:Polymorphism, Single-Stranded Conformational; D012756:Sheep / Q000033:anatomy & histology / Q000235:genetics*
D017931:DNA Primers; D009124:Muscle Proteins; D004247:DNA; D002154:Calpain