Development of reagents for differential protein quantitation by subtractive parent (precursor) ion scanning.
We present a generic approach for quantitative differential proteomics that reduces data complexity in proteome analysis by automated selection of peptides for MS/MS analysis according to their isotope-labeling ratio. Isotopic reagents were developed that give products which fragment easily to generate a unique pair of signature ions. Using the ion-pair ratio, we show that it is possible to select only BSA peptides (with a 3:1 light heavy isotope ratio) for MS/MS when spiked in a whole yeast extract using Parent (precursor) Ion Quantitation Scanning (PIQS) for MS/MS.
Wåhlander
Asa
A
Department of Protein Technology, Lund University, Sweden.
Arrigoni
Giorgio
G
Wårell
Kristofer
K
Levander
Fredrik
F
Palmgren
Ronnie
R
Maloisel
Jean-Luc
JL
Busson
Philippe
P
James
Peter
P
D000818:Animals; D007202:Indicators and Reagents; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000302:isolation & purification; D053719:Tandem Mass Spectrometry / Q000379:methods*; D015003:Yeasts
D007202:Indicators and Reagents; D011506:Proteins; D012710:Serum Albumin, Bovine