The cytosolic ribonuclease inhibitor contributes to intracellular redox homeostasis.
The hypothesis that the cytosolic RNase inhibitor (cRI) has a role in the protection of the cellular redox homeostasis was investigated testing the effects of oxidants and anti-oxidants on normal, primary endothelial HUVE cells, and malignant HeLa cells, before and after their engineering into cRI-deprived cells. We found that cRI plays an important, possibly a key, physiological role in the protection of cells from redox stress, as demonstrated by decreased GSH levels as well as increased oxidant-induced DNA damage in cRI deprived cells.
Monti
Daria Maria
DM
Department of Structural and Functional Biology, University of Naples Federico II, Complesso Universitario M.S.Angelo, via Cinthia, 80126, Naples, Italy.
Montesano Gesualdi
Nicola
N
Matousek
Josef
J
Esposito
Franca
F
D'Alessio
Giuseppe
G
D000975:Antioxidants / Q000378:metabolism; D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D006367:HeLa Cells; D006706:Homeostasis; D006801:Humans; D010084:Oxidation-Reduction; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D012260:Ribonucleases / Q000037:antagonists & inhibitors*
D000975:Antioxidants; D002352:Carrier Proteins; D004791:Enzyme Inhibitors; D034741:RNA, Small Interfering; C501467:RNH1 protein, human; D012260:Ribonucleases; D005978:Glutathione