title: Ensifer mexicanus sp. nov. a new species nodulating Acacia angustissima (Mill.) Kuntze in Mexico.
abstract: A new lineage of Ensifer nodulating the American legume Acacia angustissima in the tropical forest of Chiapas and Morelos, Mexico is described. Bacteria were identified as Ensifer with ssb or nolR specific primers. Phylogenetic analysis with partial sequences of the five chromosomal genes gyrA, nolR, recA, rpoB and rrs revealed that this new lineage is related to African Ensifer terangae. The results of total DNA-DNA hybridization and selected phenotypic tests among the A. angustissima strains and E. terangae indicated that they belong to different species. The phylogeny with the symbiotic nifH gene also separates this group as a different clade but with close affinities to bacteria belonging to the genus Ensifer isolated from American hosts. ITTG R7(T) (=CFN ER1001, HAMBI 2910, CIP 109033, ATCC BAA-1312, DSM18446) is the type strain of a new species for which the name Ensifer mexicanus sp. nov. is proposed.
authors.affiliation: Centro de Ciencias Genómicas, UNAM, Apdo. Postal 565-A, CP 62251, Cuernavaca, Morelos, Mexico.
authors.forename: Lourdes
authors.initials: L
authors.lastname: Lloret
authors.forename: Ernesto
authors.initials: E
authors.lastname: Ormeño-Orrillo
authors.forename: Reiner
authors.initials: R
authors.lastname: Rincón
authors.forename: Julio
authors.initials: J
authors.lastname: Martínez-Romero
authors.forename: Marco Antonio
authors.initials: MA
authors.lastname: Rogel-Hernández
authors.forename: Esperanza
authors.initials: E
authors.lastname: Martínez-Romero
mesh_terms: D000045:Acacia / Q000382:microbiology*; D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000235:genetics; D002876:Chromosomes, Bacterial / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D008800:Mexico; D009586:Nitrogen Fixation / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D017385:Sequence Homology; D020579:Sinorhizobium / Q000145:classification; D013045:Species Specificity; D013559:Symbiosis
chemical_list: D001426:Bacterial Proteins; C084296:nifL protein, Bacteria