title: Impact of aluminum on the oxidation of lipids and enzymatic lipolysis in monomolecular films at the air/water interface.
abstract: There is evidence that serious pathologies are associated with aluminum (Al). In the present work, the influence of Al on enzymatic lipolysis was studied with the aim to get more insight into the possible link between the Al-induced membrane modification and the cytotoxicity of the trivalent cation (AlIII). Lipid monolayers were used as model membranes. The monomolecular film technique allowed monitoring the Al-dependent modifications of the lipid monolayer properties and enzyme kinetics. Two enzymes, namely, Candida rugosa lipase and a calcium (CaII)-dependent phospholipase A2 from porcine pancreas, were used to catalyze the lipolysis of triglyceride and phosphoglyceride monolayers, respectively. The results obtained show that Al modifies both the monolayer structure and enzymatic reaction rates. While the enzymes used in this study can be considered as probes detecting lipid membrane properties, it cannot be excluded that in physiological conditions modulation of the enzyme action by the Al-bound membranes is among the reasons for Al toxicity.
authors.affiliation: Groupe d'Etude des Vecteurs Supramoléculaires du Médicament UMR 7565 CNRS/Université Henri Poincaré Nancy 1, Faculté des Sciences, BP 239, 54506 Vandoeuvre-lés-Nancy cedex, France.
authors.forename: Yohann
authors.initials: Y
authors.lastname: Corvis
authors.forename: Beata
authors.initials: B
authors.lastname: Korchowiec
authors.forename: Gerald
authors.initials: G
authors.lastname: Brezesinski
authors.forename: Sébastien
authors.initials: S
authors.lastname: Follot
authors.forename: Ewa
authors.initials: E
authors.lastname: Rogalska
mesh_terms: D000388:Air; D000535:Aluminum / Q000737:chemistry*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002175:Candida / Q000201:enzymology; D004798:Enzymes / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry; D008055:Lipids / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry; D010741:Phospholipases A / Q000737:chemistry; D054467:Phospholipases A2; D013499:Surface Properties; D013552:Swine; D014280:Triglycerides / Q000737:chemistry; D014867:Water / Q000737:chemistry*
chemical_list: D004798:Enzymes; D008055:Lipids; D010713:Phosphatidylcholines; D014280:Triglycerides; D014867:Water; D000535:Aluminum; D008049:Lipase; D010741:Phospholipases A; D054467:Phospholipases A2; D010100:Oxygen; D002118:Calcium; C028694:1-palmitoyl-2-oleoylphosphatidylcholine