Expression of Aspergillus oryzae phytase gene in Aspergillus oryzae RIB40 niaD(-).
Aspergillus oryzae RIB40 niaD(-) was transformed using a plasmid constructed with the A. oryzae phytase gene and pNAN8142 vector. The culture broth of the transformant, which was grown in a medium containing starch as a carbon source and polyvinylpyrrolidone showed phytase activity of a maximum of 2.0 units ml(-1) at 37 degrees C, pH 5.5.
Uchida
Hiroyuki
H
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, University of Fukui, 9-1 Bunkyo 3-chome, Fukui-shi 910-8507, Japan. uchida@acbio.fukui-u.ac.jp
Arakida
Shinya
S
Sakamoto
Tatsuji
T
Kawasaki
Haruhiko
H
D010832:6-Phytase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D001236:Aspergillus oryzae / Q000145:classification* / Q000502:physiology*; D004789:Enzyme Activation; D015870:Gene Expression / Q000502:physiology; D030821:Plants, Genetically Modified / Q000201:enzymology*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism; D013045:Species Specificity
D011994:Recombinant Proteins; D010832:6-Phytase