title: Ectopic brown adipose tissue in muscle provides a mechanism for differences in risk of metabolic syndrome in mice.
abstract: C57BL/6 (B6) mice subjected to a high-fat diet develop metabolic syndrome with obesity, hyperglycemia, and insulin resistance, whereas 129S6/SvEvTac (129) mice are relatively protected from this disorder because of differences in higher basal energy expenditure in 129 mice, leading to lower weight gain. At a molecular level, this difference correlates with a marked higher expression of uncoupling protein 1 (UCP1) and a higher degree of uncoupling in vitro in mitochondria isolated from muscle of 129 versus B6 mice. Detailed histological examination, however, reveals that this UCP1 is in mitochondria of brown adipocytes interspersed between muscle bundles. Indeed, the number of UCP1-positive brown fat cells in intermuscular fat in 129 mice is >700-fold higher than in B6 mice. These brown fat cells are subject to further up-regulation of UCP1 after stimulation with a beta3-adrenergic receptor agonist. Thus, ectopic deposits of brown adipose tissue in intermuscular depots with regulatable expression of UCP1 provide a genetically based mechanism of protection from weight gain and metabolic syndrome between strains of mice.
authors.affiliation: Research Division, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Katrine
authors.initials: K
authors.lastname: Almind
authors.forename: Monia
authors.initials: M
authors.lastname: Manieri
authors.forename: William I
authors.initials: WI
authors.lastname: Sivitz
authors.forename: Saverio
authors.initials: S
authors.lastname: Cinti
authors.forename: C Ronald
authors.initials: CR
authors.lastname: Kahn
mesh_terms: D002001:Adipose Tissue, Brown / Q000737:chemistry / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D000818:Animals; D004041:Dietary Fats / Q000008:administration & dosage / Q000009:adverse effects; D004734:Energy Metabolism; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D024821:Metabolic Syndrome / Q000209:etiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D009132:Muscles / Q000737:chemistry / Q000648:ultrastructure; D012306:Risk; D000071256:Uncoupling Protein 1; D015854:Up-Regulation / Q000235:genetics; D015430:Weight Gain
chemical_list: D004041:Dietary Fats; D007473:Ion Channels; D024101:Mitochondrial Proteins; C000604372:Ucp1 protein, mouse; D000071256:Uncoupling Protein 1