Effects of continuous ingestion of herbal teas on intestinal CYP3A in the rat.
Tenryocha, rooibos, and guava teas are widely consumed as herbal beverages, especially as a therapy against pollen allergy. To investigate the possible herbal tea-drug interaction the effect of continuous ingestion of these teas on cytochrome P450 (CYP) 3A were studied. Rats (n = 6) were allowed free access to either tea (experimental groups) or water (control) for two weeks. Midazolam (MDZ) (20 mg/kg) was orally administered and the serum concentration was determined. The area under the serum concentration-time curve (AUC(0-infinity)) and the maximum serum concentrations (C(max)) of MDZ were reduced by more than 60% after the treatment of tenryocha and rooibos tea (P<0.05). Intestinal MDZ 1'- and 4-hydroxylation activities mediated by CYP3A were increased in tenryocha and rooibos tea-treated group by 50% compared to the control group, although the results were not statistically significant. Furthermore, the Western blot analysis showed that CYP3A content was significantly increased in the intestine after the treatment of these teas (P<0.05). Hepatic MDZ hydroxylation and CYP3A content were slightly increased by these teas. The results suggested that two weeks ingestion of tenryocha and rooibos tea reduced serum concentration of MDZ by the induction of intestinal CYP3A. The possible interaction between tenryocha or rooibos tea and medicines mediated by CYP3A was suggested.
Matsuda
Kazuhiro
K
Department of Pharmacology, School of Medicine, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
Nishimura
Yuki
Y
Kurata
Norimitsu
N
Iwase
Mariko
M
Yasuhara
Hajime
H
D000818:Animals; D019540:Area Under Curve; D015153:Blotting, Western; D051544:Cytochrome P-450 CYP3A / Q000378:metabolism*; D018757:GABA Modulators / Q000097:blood / Q000494:pharmacology; D006207:Half-Life; D007422:Intestines / Q000187:drug effects* / Q000201:enzymology*; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D008874:Midazolam / Q000097:blood / Q000494:pharmacology; D028321:Plant Preparations / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D018757:GABA Modulators; D028321:Plant Preparations; D051544:Cytochrome P-450 CYP3A; D008874:Midazolam