Distinct sites on the Sindbis virus RNA-dependent RNA polymerase for binding to the promoters for the synthesis of genomic and subgenomic RNA.
Sindbis virus-infected cells make two positive-strand RNAs, a genomic (G) RNA and a subgenomic (SG) RNA. Here we report the amino acid sequence in nonstructural protein 4 (nsP4), the viral RNA-dependent RNA polymerase, that binds to the promoter for the synthesis of G RNA. In addition, using a cell-free system that makes both G and SG RNA, we show that specific amino acid changes in nsP4 that abolish the synthesis of SG RNA have no effect on the synthesis of G RNA. Our findings indicate that nsP4 has distinct sites for the recognition of the G and SG promoters.
Li
Mei-Ling
ML
Department of Molecular Genetics, Microbiology, and Immunology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA.
Stollar
Victor
V
D020816:Amino Acid Motifs; D012321:DNA-Directed RNA Polymerases; D016679:Genome, Viral; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012313:RNA; D012367:RNA, Viral / Q000096:biosynthesis*; D012845:Sindbis Virus / Q000235:genetics / Q000502:physiology*; D017361:Viral Nonstructural Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
D012367:RNA, Viral; D017361:Viral Nonstructural Proteins; C499947:nsP4 protein, Sindbis virus; D012313:RNA; D012321:DNA-Directed RNA Polymerases