title: Composite organic-inorganic nanoparticles as Raman labels for tissue analysis.
abstract: Composite organic-inorganic nanoparticles (COINs) are novel optical labels for detection of biomolecules. We have previously developed methods to encapsulate COINs and to functionalize them with antibodies. Here we report the first steps toward application of COINs to the detection of proteins in human tissues. Two analytes, PSA and CK18, are detected simultaneously using two different COINs in a direct binding assay, and two different COINs are shown to simultaneously label PSA in tissue samples.
authors.affiliation: Biomedical/Life Sciences, Digital Health Group, Intel Corporation, SC3-41, 2200 Mission College Boulevard, Santa Clara, California 95054, USA.
authors.forename: Lei
authors.initials: L
authors.lastname: Sun
authors.forename: Kung-Bin
authors.initials: KB
authors.lastname: Sung
authors.forename: Claire
authors.initials: C
authors.lastname: Dentinger
authors.forename: Barry
authors.initials: B
authors.lastname: Lutz
authors.forename: Lienchi
authors.initials: L
authors.lastname: Nguyen
authors.forename: Jingwu
authors.initials: J
authors.lastname: Zhang
authors.forename: Haoyu
authors.initials: H
authors.lastname: Qin
authors.forename: Mineo
authors.initials: M
authors.lastname: Yamakawa
authors.forename: Manqiu
authors.initials: M
authors.lastname: Cao
authors.forename: Yu
authors.initials: Y
authors.lastname: Lu
authors.forename: A J
authors.initials: AJ
authors.lastname: Chmura
authors.forename: Jing
authors.initials: J
authors.lastname: Zhu
authors.forename: Xing
authors.initials: X
authors.lastname: Su
authors.forename: Andrew A
authors.initials: AA
authors.lastname: Berlin
authors.forename: Selena
authors.initials: S
authors.lastname: Chan
authors.forename: Beatrice
authors.initials: B
authors.lastname: Knudsen
mesh_terms: D000906:Antibodies; D004797:Enzyme-Linked Immunosorbent Assay; D006651:Histocytochemistry / Q000379:methods; D006801:Humans; D053538:Keratin-18 / Q000032:analysis; D008297:Male; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D011467:Prostate / Q000737:chemistry; D017430:Prostate-Specific Antigen / Q000032:analysis; D011485:Protein Binding; D013059:Spectrum Analysis, Raman
chemical_list: D000906:Antibodies; D053538:Keratin-18; D017430:Prostate-Specific Antigen