Lycium barbarum polysaccharides regulate phenotypic and functional maturation of murine dendritic cells.
Lycium barbarum polysaccharides (LBPs) have been known to have a variety of immunomodulatory functions including activation of T cells, B cells and NK cells. Dendritic cells (DC) are potent antigen-presenting cells that play pivotal roles in the initiation of the primary immune response. However, little is known about the immunomodulatory effects of LBPs on murine bone marrow derived dendritic cells (BMDC). In the present study, the effects of LBPs on the phenotypic and functional maturation of murine BMDC were investigated in vitro. Compared to the BMDC that were only subjected to treatment with RPMI1640, the co-expression of I-A/I-E, CD11c and secretion of IL-12 p40 by BMDC stimulated with LBPs (100 microg/ml) were increased. In addition, the endocytosis of FITC-dextran by LBPs-treated BMDC (100 microg/ml) was impaired, whereas the activation of proliferation of allogenic lymphocytes by BMDC was enhanced. Our results strongly suggest that LBPs are capable of promoting both the phenotypic and functional maturation of murine BMDC in vitro.
Zhu
Jie
J
Department of Biochemistry and Molecular Biology, College of Medicine, Zhejiang University, Hangzhou 310006, Republic of China.
Zhao
Lu-Hang
LH
Zhao
Xiao-Ping
XP
Chen
Zhi
Z
D000818:Animals; D000941:Antigens / Q000276:immunology; D039521:CD11c Antigen / Q000276:immunology; D049109:Cell Proliferation / Q000187:drug effects; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology*; D003911:Dextrans / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D053762:Interleukin-12 Subunit p40 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D051379:Mice; D010641:Phenotype; D015854:Up-Regulation / Q000187:drug effects
D000941:Antigens; D039521:CD11c Antigen; D003911:Dextrans; D004365:Drugs, Chinese Herbal; D053762:Interleukin-12 Subunit p40; D008070:Lipopolysaccharides; C500223:lycium barbarum polysaccharide; D016650:Fluorescein-5-isothiocyanate