title: Monitoring the reaction of hemoglobin with hydrogen peroxide by capillary electrophoresis-chemiluminescence detection.
abstract: The reaction of hemoglobin (Hb) with hydrogen peroxide (H2O2) leads to fluorescent product and heme degradation. We applied capillary electrophoresis-chemiluminescence (CE-CL) detection to monitor the course of Hb reacting with H2O2. Hb and released free iron ion (Fe3+) were detected based on their enhancement effects on CL of the luminol-H2O2 system. In this study, we discovered an intermediate of this reaction which intensely enhances the luminol-H2O2 CL system. The ratio of max CL signals of Fe3+, Hb and this intermediate is circa 1:10:60.
authors.affiliation: Department of Chemistry, Wuhan University, Wuhan 430072, China.
authors.forename: Shi-Lai
authors.initials: SL
authors.lastname: Zhou
authors.forename: Jun-Hua
authors.initials: JH
authors.lastname: Wang
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Huang
authors.forename: Xin
authors.initials: X
authors.lastname: Lu
authors.forename: Jie-Ke
authors.initials: JK
authors.lastname: Cheng
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D004912:Erythrocytes / Q000737:chemistry; D006454:Hemoglobins / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry*; D049449:Luminescence
chemical_list: D006454:Hemoglobins; D006861:Hydrogen Peroxide