[Quantification of PPARdelta mRNA by real-time RT-PCR in rectal cancer tissues].
OBJECTIVE
To clarify the expression change of PPARdelta gene in human rectal cancer tissues and determine the correlation of PPARdelta expression with the clinical and pathological parameters of rectal cancer.


METHODS
Applying real-time RT-PCR, we quantified PPARdelta mRNA in 86 tissues from excised primary rectal cancers. In each case, accompanying normal mucosa was collected for comparison.


RESULTS
Among the 86 rectal cancer tissues, 48 (55.8%) cases showed PPARdelta overexpression: 39 (81.3%) tumors gave an expression level 1.5 to 5.0 times, 5 (10.4%) tumors 10 to 20 times, and 4 (8.3%) tumors more than 20 times relative to normal mucosa. However, the general level of PPARdelta mRNA in rectal cancer tissues is not statistically different from that in normal mucosa. There was no evidence for the relationships of PPARdelta expression with cell differentiation, pathological categories and Dukes stages.


CONCLUSIONS
The expression of PPARdelta gene in rectal cancers is not statistically different from that in normal mucosa, and it is not correlated with cell differentiation, pathological categories and Dukes stages.
Yang
Lie
L
Division of Digestive Surgery and Organ-Microcirculation, West China Hospital, Sichuan University, Chengdu 610041, China.
Yu
Yong-yang
YY
Zhou
Zong-guang
ZG
Luo
Hong-zhi
HZ
Zhou
Bin
B
Tian
Chao
C
Gu
Jun
J
D002454:Cell Differentiation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009367:Neoplasm Staging; D047494:PPAR delta / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012004:Rectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
D047494:PPAR delta; D012333:RNA, Messenger