title: Upregulation of Slc39a10 gene expression in response to thyroid hormones in intestine and kidney.
abstract: A novel zinc transporter has been purified and cloned from rat renal brush border membrane. This transporter was designated as Zip10 encoded by Slc39a10 gene and characterized as zinc importer. Present study documents the impact of thyroid hormones on the expression of Zip10 encoded by Slc39a10 gene in rat model of hypo and hyperthyroidism. Serum T(3) and T(4) levels were reduced significantly in hypothyroid rats whereas these levels were significantly elevated in hyperthyroid rats as compared to euthyroid rats thereby confirming the validity of the model. Kinetic studies revealed a significant increase in the initial and equilibrium uptake of Zn(++) in both intestinal and renal BBMV of hyperthyroid rats in comparison to hypothyroid and euthyroid rats. By RT-PCR, Slc39a10 mRNA expression was found to be significantly decreased in hypothyroid and increased in hyperthyroid as compared to euthyroid rats. These findings are in conformity with the immunofluorescence studies that revealed markedly higher fluorescence intensity at periphery of both intestinal and renal cells isolated from hyperthyroid rats as compared to hypothyroid and euthyroid rats. Higher expression of Zip10 protein in hyperthyroid group was also confirmed by western blot. These findings suggest that expression of zinc transporter protein Zip10 (Slc39a10) in intestine and kidney is positively regulated by thyroid hormones.
authors.affiliation: Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh-160012, India.
authors.forename: Kaler
authors.initials: K
authors.lastname: Pawan
authors.forename: Sharma
authors.initials: S
authors.lastname: Neeraj
authors.forename: Kumar
authors.initials: K
authors.lastname: Sandeep
authors.forename: Radha
authors.initials: R
authors.lastname: Kanta Ratho
authors.forename: Prasad
authors.initials: P
authors.lastname: Rajendra
mesh_terms: D000818:Animals; D027682:Cation Transport Proteins / Q000235:genetics* / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006980:Hyperthyroidism / Q000235:genetics / Q000378:metabolism; D007037:Hypothyroidism / Q000235:genetics / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008668:Metallothionein / Q000378:metabolism; D023421:Models, Animal; D051381:Rats; D017208:Rats, Wistar; D013963:Thyroid Hormones / Q000378:metabolism*; D015854:Up-Regulation; D015032:Zinc / Q000378:metabolism
chemical_list: D027682:Cation Transport Proteins; C517001:Slc39a10 protein, rat; D013963:Thyroid Hormones; D008668:Metallothionein; D015032:Zinc