title: Diffusion tensor imaging of native and degenerated human articular cartilage.
abstract: Diffusion tensor imaging (DTI) is potentially sensitive to collagen degeneration in cartilage. In this study, DTI was measured on human cartilage samples with interventions of trypsin and collagenase. The measured preferred diffusion direction was consistent with the zonal structure of collagen network. The glycosaminoglycan concentration decreased and apparent diffusion coefficient increased with both interventions. The fractional anisotropy (FA) was not affected by trypsin and showed a slight increase with combined trypsin and collagenase intervention. DTI in cartilage is technically challenging due to the low FA and the almost undetectable change with collagen disruption seen here.
authors.affiliation: Department of Radiology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Xiang
authors.initials: X
authors.lastname: Deng
authors.forename: Michelle
authors.initials: M
authors.lastname: Farley
authors.forename: Miika T
authors.initials: MT
authors.lastname: Nieminen
authors.forename: Martha
authors.initials: M
authors.lastname: Gray
authors.forename: Deborah
authors.initials: D
authors.lastname: Burstein
mesh_terms: D002102:Cadaver; D002358:Cartilage, Articular / Q000737:chemistry / Q000473:pathology*; D017364:Collagenases / Q000032:analysis; D038524:Diffusion Magnetic Resonance Imaging / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D005269:Femur; D006025:Glycosaminoglycans / Q000032:analysis; D006801:Humans; D007091:Image Processing, Computer-Assisted; D020781:Matrix Metalloproteinase 1 / Q000032:analysis; D014357:Trypsin / Q000032:analysis
chemical_list: D006025:Glycosaminoglycans; D014357:Trypsin; D017364:Collagenases; D020781:Matrix Metalloproteinase 1