Comparison of transformation efficiency of piggyBac transposon among three different silkworm Bombyx mori Strains.
The transformation rate of three different strains of silkworm Bombyx mori was compared after the introduction of enhanced green fluorescence protein (EGFP)-encoding genes into the silkworm eggs by microinjection of a mixture of piggyBac vector and helper plasmid containing a transposase-encoding sequence. Although there were no significant differences among the three strains in the percentages of fertile moths in microinjected eggs (P=0.1258), the percentages of G(0) transformed moths in fertile moths and injected eggs were both significantly different (P=0.01368 and P=0.02398, respectively). The transformation rate of the Nistari strain (Indian strain) was significantly higher than that of the other two strains, Golden-yellow-cocoon (Vietnamese strain) and Jiaqiu (Chinese strain), which had similar rate. These results indicate that the transformation efficiency of the piggyBac-based system might vary with silkworm strains with different genetic backgrounds. The presence of endogenous piggyBac-like elements might be an important factor influencing the transformation efficiency of introduced piggyBac-derived vectors, and the diverse amount and activation in different silkworm strains might account for the significant differences.
Zhong
Boxiong
B
College of Animal Sciences, Zhejiang University, Hangzhou 310029, China. bxzhong@zju.edu.cn
Li
Jianying
J
Chen
Jin'e
J
Ye
Jian
J
Yu
Songdong
S
D000818:Animals; D012831:Bombyx / Q000145:classification / Q000235:genetics*; D002681:China; D017931:DNA Primers; D004251:DNA Transposable Elements / Q000235:genetics*; D005260:Female; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D007194:India; D010063:Ovum / Q000502:physiology; D010957:Plasmids; D016133:Polymerase Chain Reaction; D014170:Transformation, Genetic; D014744:Vietnam
D017931:DNA Primers; D004251:DNA Transposable Elements; D049452:Green Fluorescent Proteins