Anti-inflammatory and lysosomal stability actions of Cleome gynandra L. studied in adjuvant induced arthritic rats.
The present study was aimed to assess the anti-arthritic nature of Cleome gynandra L. (Cat's whiskers) against Freund's complete adjuvant induced arthritis in rats. The ethanolic extract of C. gynandra was administered orally at a dose of 150 mg/kg body weight for 30 days to the experimental rats after the induction of adjuvant arthritis. The anti-inflammatory activity of C. gynandra leaves was assessed by paw volume measurement, and its capacity to stabilize lysosomal enzyme activities in the plasma and liver of control and experimental rats. The activity of pathophysiological enzymes such as AST, ALT, ALP, cathepsin-D, beta-glucuronidase, N-acetyl-beta-glucosaminidase LDH and the levels of glycoproteins were also estimated in plasma and liver. The increased levels of both lysosomal enzymes and protein-bound carbohydrates in arthritic rats were significantly suppressed to near normal level by the administration of C. gynandra extract. Further, the significantly elevated plasma levels of TNF-alpha found in arthritic rats were found to be significantly restored back to near normal levels by the extract in experimental animals. The membrane stabilizing activity of the extract was further evidenced by histological observations made on the limb tissue. Recently, we have reported the presence of many biologically active phyto chemicals such as triterpenes, tannins, anthroquinones, flavonoids, saponins, steroids, resins, lectins, glycosides, sugars, phenolic compounds, and alkaloids in the extract of C. gynandra and these compounds might be responsible for the anti-arthritic properties observed in the present study. The possible mechanism of action of the C. gynandra extract may be through its stabilizing action on lysosomal membranes and there by preventing the spread of inflammation.
Narendhirakannan
R T
RT
Department of Inorganic Chemistry, University of Madras, Guindy Campus, Chennai 600025, Tamil Nadu, India.
Subramanian
S
S
Kandaswamy
M
M
D000818:Animals; D001169:Arthritis, Experimental / Q000188:drug therapy* / Q000201:enzymology / Q000378:metabolism; D001172:Arthritis, Rheumatoid / Q000188:drug therapy* / Q000201:enzymology / Q000378:metabolism; D002402:Cathepsin D / Q000378:metabolism; D002462:Cell Membrane / Q000201:enzymology; D031485:Cleome / Q000737:chemistry*; D005528:Foot / Q000473:pathology; D005620:Freund's Adjuvant; D005643:Fucose / Q000378:metabolism; D006595:Hexosamines / Q000378:metabolism; D006601:Hexoses / Q000378:metabolism; D006603:Hexuronic Acids / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008247:Lysosomes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000378:metabolism
D006595:Hexosamines; D006601:Hexoses; D006603:Hexuronic Acids; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D005643:Fucose; D005620:Freund's Adjuvant; D002402:Cathepsin D; D019158:N-Acetylneuraminic Acid