title: A derepression system based on the Bacillus subtilis sporulation pathway offers dynamic control of heterologous gene expression.
abstract: By rewiring the sporulation gene-regulatory network of Bacillus subtilis, we generated a novel expression system relying on derepression. The gene of interest is placed under the control of the abrB promoter, which is active only when Spo0A is absent, and Spo0A is controlled via an IPTG (isopropyl-beta-d-thiogalactopyranoside)-inducible promoter.
authors.affiliation: Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands.
authors.forename: Reindert
authors.initials: R
authors.lastname: Nijland
authors.forename: Jan-Willem
authors.initials: JW
authors.lastname: Veening
authors.forename: Oscar P
authors.initials: OP
authors.lastname: Kuipers
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics* / Q000502:physiology; D001426:Bacterial Proteins / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D005434:Flow Cytometry; D015964:Gene Expression Regulation, Bacterial; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D013171:Spores, Bacterial / Q000502:physiology; D014121:Toxoids / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics
chemical_list: C060762:AbrB protein, Bacillus subtilis; D001426:Bacterial Proteins; C035454:Clostridium perfringens toxoid; D004268:DNA-Binding Proteins; D014121:Toxoids; D014157:Transcription Factors; D007544:Isopropyl Thiogalactoside