title: Identification and characterization of a functional Candida albicans homolog of the Saccharomyces cerevisiae TCO89 gene.
abstract: As one of the components of target of rapamycin complex 1 (TORC1), ScTco89p is involved in rapamycin sensitivity and cellular integrity in Saccharomyces cerevisiae. Here we provide evidence showing that deletion of ScTCO89 causes yeast cells to be hypersensitive to salt stress in a high osmolarity glycerol pathway-independent fashion. In addition, we have identified and characterized a functional Candida albicans homolog (CaTCO89) of ScTCO89, which encodes a protein of 708 amino acids that shows overall 15% identity with ScTco89p at the amino acid level. However, CaTCO89 could complement the functions of ScTCO89 in rapamycin sensitivity, salt tolerance, and cellular integrity. Candida albicans cells disrupted for CaTCO89 are also sensitive to rapamycin and lithium salt, but not susceptible to challenges to cellular integrity.
authors.affiliation: Laboratory of Molecular Microbiology, Department of Molecular and Cellular Pharmacology, College of Pharmaceuticals and Biotechnology, Tianjin University, Tianjin, China.
authors.forename: Changlong
authors.initials: C
authors.lastname: Zheng
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Yan
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Linghuo
authors.initials: L
authors.lastname: Jiang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002176:Candida albicans / Q000235:genetics* / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017353:Gene Deletion; D018020:Lithium Compounds / Q000378:metabolism; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D020123:Sirolimus / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D018020:Lithium Compounds; D029701:Saccharomyces cerevisiae Proteins; C485218:Tco89 protein, S cerevisiae; D020123:Sirolimus