title: The DYNLT3 light chain directly links cytoplasmic dynein to a spindle checkpoint protein, Bub3.
abstract: Cytoplasmic dynein is the motor protein responsible for the intracellular transport of various organelles and other cargoes toward microtubule minus ends. However, it remains to be determined how dynein is regulated to accomplish its varied roles. The dynein complex contains six subunits, including three classes of light chains. The two isoforms of the DYNLT (Tctex1) family of light chains, DYNLT1 and DYNLT3, have been proposed to link dynein to specific cargoes. However, no specific binding partner had been found for the DYNLT3 light chain. We find that DYNLT3 binds to Bub3, a spindle checkpoint protein. Bub3 binds exclusively to DYNLT3 and not to the other dynein light chains. Glutathione S-transferase pull-down and co-immunoprecipitation assays demonstrate that Bub3 interacts with the cytoplasmic dynein complex. DYNLT3 is present on kinetochores at prometaphase, but not later mitotic stages, demonstrating that this dynein light chain, like Bub3 and other checkpoint proteins, is depleted from the kinetochore during chromosome alignment. Knockdown of DYNLT3 with small interference RNA increases the mitotic index, in particular, the number of cells in prophase/prometaphase. These results demonstrate that dynein binds directly to a component of the spindle checkpoint complex through the DYNLT3 light chain. Thus, DYNLT3 contributes to dynein cargo binding specificity. These data also suggest that the subpopulation of dynein, containing the DYNLT3 light chain, may be important for chromosome congression, in addition to having a role in the transport of checkpoint proteins from the kinetochore to the spindle pole.
authors.affiliation: Department of Cell Biology, School of Medicine, University of Virginia, Charlottesville, Virginia 22908-0732, USA.
authors.forename: Kevin W-H
authors.initials: KW
authors.lastname: Lo
authors.forename: John M
authors.initials: JM
authors.lastname: Kogoy
authors.forename: K Kevin
authors.initials: KK
authors.lastname: Pfister
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002522:Chlorocebus aethiops; D002868:Chromosomal Proteins, Non-Histone; D003593:Cytoplasm / Q000378:metabolism*; D004398:Dyneins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D018386:Kinetochores / Q000378:metabolism; D051379:Mice; D008938:Mitosis; D000075223:Poly-ADP-Ribose Binding Proteins; D011485:Protein Binding; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats
chemical_list: C489444:BUB3 protein, human; C489445:Bub3 protein, mouse; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D000075223:Poly-ADP-Ribose Binding Proteins; D034741:RNA, Small Interfering; C519849:DYNLT3 protein, mouse; C519850:DYNLT3 protein, rat; D004398:Dyneins