Crystallization of the archaeal transcription termination factor NusA: a significant decrease in twinning under microgravity conditions.
The transcription termination factor NusA from Aeropyrum pernix was crystallized using a counter-diffusion technique in both terrestrial and microgravity environments. Crystallization under microgravity conditions significantly reduced the twinning content (1.0%) compared with terrestrially grown crystals (18.3%) and improved the maximum resolution from 3.0 to 2.29 A, with similar unit-cell parameters. Based on a comparison of the crystal parameters, the effect of microgravity on protein crystallization is discussed.
Tanaka
Hiroaki
H
Confocal Science Inc., Wakamatsu Building 7F, 3-3-6 Nihonbashi Hon-cho, Chuo-ku, Tokyo 103-0023, Japan.
Umehara
Takashi
T
Inaka
Koji
K
Takahashi
Sachiko
S
Shibata
Rie
R
Bessho
Yoshitaka
Y
Sato
Masaru
M
Sugiyama
Shigeru
S
Fusatomi
Emiko
E
Terada
Takaho
T
Shirouzu
Mikako
M
Sano
Satoshi
S
Motohara
Moritoshi
M
Kobayashi
Tomoyuki
T
Tanaka
Tetsuo
T
Tanaka
Akiko
A
Yokoyama
Shigeyuki
S
D041522:Aeropyrum / Q000737:chemistry*; D019843:Archaeal Proteins / Q000737:chemistry*; D018360:Crystallography, X-Ray; D010445:Peptide Elongation Factors / Q000737:chemistry*; D014157:Transcription Factors / Q000737:chemistry*
D019843:Archaeal Proteins; D010445:Peptide Elongation Factors; D014157:Transcription Factors