[Simultaneous determination of residues of 12 amide herbicides in soybeans by high performance liquid chromatography].
An analytical method was developed for the simultaneous determination of 12 amide herbicide residues in soybeans. The 12 amide herbicides were extracted with acetone from soybean samples. The extract was cleaned up using a Florisil cartridge, and then determined by high performance liquid chromatography. Kromasil 100-5 C18 column (4.6 mm i. d. x 250 mm, 5 microm) as analytical column, pure water-acetonitrile as mobile phase with a flow rate of 1.0 mL/min and gradient elution, and a diode array detector set at 210 nm were used. The recoveries of amide herbicides from soybeans spiked at levels of 0.05, 0.1 and 1.0 mg/kg were in the range of 75% - 102% with relative standard deviations of 1.9% - 16.1%. The method has good linear in the range of 0.05 - 1.0 mg/L for each analyte (nu > 0.999). The detection limit for each amide herbicide was less than 0.05 mg/kg. The method is simple, rapid and accurate. It can be used for the inspection of amide herbicide residues in soybeans.
Li
Jianzhong
J
Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100025, China. leemaster_2000@sina.com
Chu
Xiaogang
X
Cai
Huixia
H
An
Juan
J
Yang
Qiang
Q
D000577:Amides / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D006540:Herbicides / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D013025:Glycine max / Q000737:chemistry*
D000577:Amides; D006540:Herbicides; D010573:Pesticide Residues