title: A new colorimetric method for determining the isomerization activity of sucrose isomerase.
abstract: A new colorimetric method for determining the isomerization activity of sucrose isomerase was developed. This colorimetric method is based on the enzymatic reactions of invertase and glucose oxidase-peroxidase (GOD-POD). The main scheme for assaying sucrose isomerase activity is to degrade sucrose in the reaction mixture to glucose and fructose by invertase and to detect the concentration of glucose generated using GOD-POD. The concentrations of trehalulose and isomaltulose, reaction products of sucrose isomerase, are calculated from the concentration of glucose. This method allows rapid and accurate determination of the isomerization activity of sucrose isomerase without inhibition by hydrolysis activity.
authors.affiliation: Institute of Life Science and Resources, Graduate School of Biotechnology, KyungHee University, Yongin, Korea.
authors.forename: Sang-Eun
authors.initials: SE
authors.lastname: Park
authors.forename: Mee-Hyun
authors.initials: MH
authors.lastname: Cho
authors.forename: Jin Kyu
authors.initials: JK
authors.lastname: Lim
authors.forename: Jong-Sang
authors.initials: JS
authors.lastname: Kim
authors.forename: Jeong Hwan
authors.initials: JH
authors.lastname: Kim
authors.forename: Dae Young
authors.initials: DY
authors.lastname: Kwon
authors.forename: Cheon-Seok
authors.initials: CS
authors.lastname: Park
mesh_terms: D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003124:Colorimetry; D005964:Glucosyltransferases / Q000737:chemistry / Q000378:metabolism*; D007536:Isomerism; D007700:Kinetics; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000737:chemistry; D013395:Sucrose / Q000737:chemistry; D043324:beta-Fructofuranosidase / Q000737:chemistry
chemical_list: D013395:Sucrose; D010544:Peroxidases; C023057:glucose peroxidase; D005964:Glucosyltransferases; C116853:sucrose isomerase; D043324:beta-Fructofuranosidase