title: Proteomic investigation of glioblastoma cell lines treated with wild-type p53 and cytotoxic chemotherapy demonstrates an association between galectin-1 and p53 expression.
abstract: Global protein analysis of treated and untreated glioblastoma cell lines was performed. Proteomic analysis revealed the identity of proteins that were significantly modulated by the treatment with wild-type TP53 and the cytotoxic chemotherapy SN38. In particular, galectin-1 was found to be negatively regulated by transfection with TP53 and further down-regulated by SN38. Expression level changes were confirmed by Western blot. Subsequent analysis of several high-grade glioma cell lines demonstrated very high levels of galectin-1, regardless if the cell lines contained mutant or wild-type TP53. High expression of galectin-1 in a human orthotopic murine tumor model was also detected by immunohistochemistry and revealed a consistent pattern of preferential expression in peripheral or leading tumor edges. Further examination of galectin-1 expression through microarray analysis in tumor materials from patients confirmed galectin-1 as a valuable biomarker and possible therapeutic target. These results demonstrate the utility of using proteomic approaches to interrogate and identify potential useful targets for cancer therapy by evaluating specific tumor responses, either positive or negative, to various therapies.
authors.affiliation: Institute of Neuroscience and Physiology, Sahlgrenska Academy, Göteborg University, SU/Mölndal, SE-43180 Mölndal, Sweden.
authors.forename: Maja
authors.initials: M
authors.lastname: Puchades
authors.forename: Carol L
authors.initials: CL
authors.lastname: Nilsson
authors.forename: Mark R
authors.initials: MR
authors.lastname: Emmett
authors.forename: Kenneth D
authors.initials: KD
authors.lastname: Aldape
authors.forename: Yongjie
authors.initials: Y
authors.lastname: Ji
authors.forename: Frederick F
authors.initials: FF
authors.lastname: Lang
authors.forename: Ta-Jen
authors.initials: TJ
authors.lastname: Liu
authors.forename: Charles A
authors.initials: CA
authors.lastname: Conrad
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D015153:Blotting, Western; D002455:Cell Division; D045744:Cell Line, Tumor; D002470:Cell Survival; D015180:Electrophoresis, Gel, Two-Dimensional; D037483:Galectin 1 / Q000235:genetics* / Q000302:isolation & purification; D015316:Genetic Therapy; D005909:Glioblastoma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D013058:Mass Spectrometry; D009368:Neoplasm Transplantation; D040901:Proteomics / Q000379:methods*; D014183:Transplantation, Heterologous; D016159:Tumor Suppressor Protein p53 / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000970:Antineoplastic Agents; D037483:Galectin 1; D016159:Tumor Suppressor Protein p53