title: Effect of bioactive peptides isolated from yeastolate, lactalbumin and NZCase in the insect cell growth.
abstract: In this study, we have described the biological activity of various hydrolysates and its effect on cell growth, growth rate and doubling time. A potent cell culture enhancer factor was observed in the yeastolate hydrolysates, mainly in the protein fractions with low molecular weight. In this case, a growth enhancer of 60.66% was obtained. Despite a lower efficiency of crude lactalbumin hydrolysates (14%), when lactalbumin and yeastolate were added together to the culture, the cell yields were of 102%, showing a synergic effect. Nevertheless, sub fraction from LMW, of lactalbumin, obtained by Sephadex G-10 gel filtration chromatography showed a higher positive effect (23.3%) than low molecular weight fraction of lactalbumin without this chromatography step (11.3%). It is suggested that low molecular weight lactalbumin could have some inhibitory protein. On the other hand, NZCase low molecular weight showed a positive effect of 29.33%, while its sub fractions showed a negative effect of 5.5%. With these data we can suggest that these hydrolysates could be an important element to design new media, serum free, being helpful in protein recombinant production.
authors.affiliation: Laboratório de Imunologia Viral, Instituto Butantan, CEP, São Paulo, SP, Brazil. zucatelli@uol.com.br
authors.forename: Ronaldo Zucatelli
authors.initials: RZ
authors.lastname: Mendonça
authors.forename: Elizabeth Cristina
authors.initials: EC
authors.lastname: de Oliveira
authors.forename: Carlos Augusto
authors.initials: CA
authors.lastname: Pereira
authors.forename: Ivo
authors.initials: I
authors.lastname: Lebrun
mesh_terms: D000818:Animals; D001709:Biotechnology; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D003470:Culture Media / Q000737:chemistry; D006868:Hydrolysis; D007768:Lactalbumin / Q000737:chemistry*; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000096:biosynthesis; D018411:Spodoptera; D015003:Yeasts / Q000737:chemistry
chemical_list: D003470:Culture Media; D010455:Peptides; D011994:Recombinant Proteins; D007768:Lactalbumin