[Effect of insulin-like growth factor I on the transdifferentiation and collagen synthesis of human renal tubular epithelial cells].
OBJECTIVE
To investigate the role of insulin-like growth factor I(IGF-I) in transdifferentiation and collagen synthesis of human renal tubular epithelial cell line HK2 in vitro.


METHODS
Cultured HK2 cells were divided into two groups: (1) control group; (2) IGF-I-treated group(25, 50, 100, 200 microg/L, respectively). The cell morphological changes were traced with inverted microscope, and the expression of alpha-smooth muscle actin (alpha-SMA), collagen I alpha and collagen III alpha mRNA was detected by RT-PCR. Concentration of collagen I secreted into the culture supernatant was determined by ELISA.


RESULTS
In HK2 cells, IGF-I stimulated the morphological oval-to-fusiform transdifferentiation of the cells, and upregulated alpha-SMA, collagen I alpha and collagen III alpha mRNA expression(P<0.05). Secreted collagen I level was also up-regulate by IGF-I in the concentration of 100 and 200 microg/L, respectively.


CONCLUSIONS
IGF-I can promote the transdifferentiation of human renal tubular epithelial cells and stimulate the synthesis of collagen.
Zhang
Ai-qing
AQ
Institute of Pediatrics, Nanjing Medical University, Nanjing 210029, China. njaiqing@163.com
Gan
Wei-hua
WH
Chen
Rong-hua
RH
Pan
Xiao-Qin
XQ
Fei
Li
L
D000199:Actins / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D054338:Cell Transdifferentiation; D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics*; D024042:Collagen Type I / Q000235:genetics; D024061:Collagen Type III / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D007684:Kidney Tubules / Q000166:cytology* / Q000187:drug effects; D020133:Reverse Transcriptase Polymerase Chain Reaction
C541116:ACTA2 protein, human; D000199:Actins; D024042:Collagen Type I; D024061:Collagen Type III; D007334:Insulin-Like Growth Factor I; D003094:Collagen