title: Nano-manipulation of single DNA molecules based on atomic force microscopy.
abstract: Nano-manipulation of single atoms and molecules is a critical technique in nanoscience and nanotechnology. This paper will focus on the recent development of the manipulation of single DNA molecules based on atomic force microscopy (AFM) in our laboratory. Precise manipulation has been realized including varied manipulating modes such as "cutting", "pushing", "folding", "kneading", "picking up", "dipping", etc. The cutting accuracy is dominated by the size of the AFM tip, which is usually 10nm or less. Single DNA fragments can be cut and picked up and then amplified by single molecule PCR. Thus positioning isolation and sequencing can be performed.
authors.affiliation: Shanghai Institute of Applied Physics, the Chinese Academy of Sciences, Shanghai 201800.
authors.forename: Lu
authors.initials: L
authors.lastname: Jun-Hong
authors.forename: Li
authors.initials: L
authors.lastname: Hai-Kuo
authors.forename: An
authors.initials: A
authors.lastname: Hong-Jie
authors.forename: Wang
authors.initials: W
authors.lastname: Guo-Hua
authors.forename: Wang
authors.initials: W
authors.lastname: Ying
authors.forename: Li
authors.initials: L
authors.lastname: Min-Qian
authors.forename: Zhang
authors.initials: Z
authors.lastname: Yi
authors.forename: Li
authors.initials: L
authors.lastname: Bin
authors.forename: Hu
authors.initials: H
authors.lastname: Jun