title: Depuration dynamics of oysters (Crassostrea gigas) artificially contaminated by Salmonella enterica serovar Typhimurium.
abstract: The State of Santa Catarina produces the greatest quantity of edible mollusks in Brazil. To guarantee sanitary qualify, mollusk cultures should be monitored for contamination by pathogenic microorganisms. A self-purification or "depuration" system that eliminates Salmonella enterica serovar Typhimurium contamination from oysters has been developed and evaluated. The depuration process occurred within a closed system, in which 1000 L of water was recirculated for 24 h. The water was sterilized with ultraviolet (UV) light, chlorine, or both together. Oysters (Crassostrea gigas) artificially contaminated with S. typhimurium were harvested every 6 h. Samples of oyster tissue were excised and both the presence and numbers of bacteria were determined. Combined UV light and chlorine treatments resulted in total elimination of bacteria within 12 h. Polymerase chain reaction detected bacteria in water exposed to the three treatments. This pioneering study is the first of its kind in Brazil and represents a major contribution to commercial mollusk culture in this country.
authors.affiliation: Laboratório de Virologia Aplicada, Departamento de Microbiologia e Parasitologia, CCB, Campus Universitario Trindade, 88040-900 Florianópolis-SC, Brazil.
authors.forename: Adriana
authors.initials: A
authors.lastname: de Abreu Corrêa
authors.forename: Jonas Dutra
authors.initials: JD
authors.lastname: Albarnaz
authors.forename: Vanessa
authors.initials: V
authors.lastname: Moresco
authors.forename: Carlos Rogério
authors.initials: CR
authors.lastname: Poli
authors.forename: Adriano Luiz
authors.initials: AL
authors.lastname: Teixeira
authors.forename: Cláudia Maria
authors.initials: CM
authors.lastname: Oliveira Simões
authors.forename: Célia Regina
authors.initials: CR
authors.lastname: Monte Barardi
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D017756:Aquaculture / Q000379:methods*; D001938:Brazil; D002713:Chlorine / Q000633:toxicity; D017931:DNA Primers; D004586:Electrophoresis; D005506:Food Contamination / Q000517:prevention & control*; D010125:Ostreidae / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics / Q000528:radiation effects*; D012758:Shellfish / Q000382:microbiology*; D014466:Ultraviolet Rays
chemical_list: D017931:DNA Primers; D002713:Chlorine