title: Oxidative stress induced by torsion of the spermatic cord in young rats.
abstract: OBJECTIVE
  To evaluate the effects of the oxidative stress in an experimental model of torsion/detorsion of the spermatic cord and the legitimacy of this model for oxidative stress studies.
  
  
  METHODS
  Forty-eight male Wistar rats were randomized in two groups (n=24): G-1 (Sham) and G-2 (Ischemia/Reperfusion). All rats received intraperitoneal saline injections (2.0 ml), at 21, 9, and 1 h before right spermatic cord torsion or first sham operation. Detorsion or second sham operation was carried out 3 h later followed by testis and blood samples collection (T-0). Additional samples were collected at 1-3-6 h time-points for assessment of testis malonaldehyde, glutathione, and plasma total antioxidant power (TAP).
  
  
  RESULTS
  Spermatic cord torsion/detorsion induced a significant increase in testicular malonaldehyde contents and a significant decrease in glutathione concentrations in ischemic rats compared with sham animals. Additional increase in malonaldehyde levels occurred during reperfusion in G-2 rats. TAP was similar in both groups denoting absence of systemic effects in this study.
  
  
  CONCLUSIONS
  Torsion/detorsion of the spermatic cord for 3 h induces significant lipid peroxidation and reduction in glutathione content of the testis and is, therefore, a valid model for studying the oxidative stress effects of the ischemia/reperfusion injury in young rat testis.
authors.affiliation: Department of Surgery, Experimental Surgical Research Laboratory, Federal University of Ceará, Rua Prof. Costa Mendes 1608, 60430-140 Fortaleza, Ceará, Brazil. mcirur@ufc.br
authors.forename: Sergio Botelho
authors.initials: SB
authors.lastname: Guimarães
authors.forename: Alan Arruda
authors.initials: AA
authors.lastname: Aragão
authors.forename: Jefferson Menezes Viana
authors.initials: JM
authors.lastname: Santos
authors.forename: Osamu de Sandes
authors.initials: Ode S
authors.lastname: Kimura
authors.forename: Paulo Hudson Uchoa
authors.initials: PH
authors.lastname: Barbosa
authors.forename: Paulo Roberto Leitão de
authors.initials: PR
authors.lastname: Vasconcelos
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis; D005978:Glutathione / Q000097:blood; D007274:Injections, Intraperitoneal; D015227:Lipid Peroxidation / Q000502:physiology; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D023421:Models, Animal; D018384:Oxidative Stress / Q000502:physiology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000150:complications; D012965:Sodium Chloride / Q000008:administration & dosage; D013086:Spermatic Cord Torsion / Q000150:complications / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D013997:Time Factors
chemical_list: D000975:Antioxidants; D017392:Thiobarbituric Acid Reactive Substances; D012965:Sodium Chloride; D008315:Malondialdehyde; D005978:Glutathione