A novel inhibitory protein in adipose tissue, the aldo-keto reductase AKR1B7: its role in adipogenesis.
The aldo-keto reductase 1B7 (AKR1B7) encodes an aldose-reductase that has been reported as a detoxification enzyme until now. We have demonstrated that AKR1B7 is differently expressed in various mouse white adipose tissues depending on their location. Its expression is associated with a higher ratio of preadipocytes vs. adipocytes. The cells that express AKR1B7 did not contain lipid droplets, and the expression level of akr1b7 was very low in mature adipocytes. We have defined the role of AKR1B7 in adipogenesis using either primary cultures of adipose stromal cells (containing adipocyte precursors) or the 3T3-L1 cell line. Under the same differentiation conditions, adipose stromal cells from tissues that expressed AKR1B7 had a decreased capacity to accumulate lipids compared with those that did not express it. Moreover, the overexpression of sense or antisense AKR1B7 in 3T3-L1 preadipocytes inhibited or accelerated, respectively, their rate of differentiation into adipocytes. In vivo experiments demonstrated that AKR1B7-encoding mRNA expression decreased in adipose tissues from mice where obesity was induced by a high-fat diet. All these results attributed for the first time a novel role to AKR1B7, which is the inhibition of adipogenesis in some adipose tissues.
Tirard
Julien
J
Institut National de la Santé et de la Recherche Médicale Unité 449-Institut National de la Recherche Agronomique Unité Mixte de Recherche 1235, Université Claude Bernard, 69000 Lyon Cedex 08, France.
Gout
Johann
J
Lefrançois-Martinez
Anne Marie
AM
Martinez
Antoine
A
Begeot
Martine
M
Naville
Danielle
D
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000201:enzymology; D050156:Adipogenesis / Q000502:physiology*; D000273:Adipose Tissue / Q000201:enzymology*; D000449:Aldehyde Reductase / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002458:Cell Fractionation; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000235:genetics; D004041:Dietary Fats / Q000494:pharmacology; D004822:Epididymis / Q000166:cytology / Q000201:enzymology; D015971:Gene Expression Regulation, Enzymologic; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000378:metabolism* / Q000503:physiopathology; D010750:Phosphoproteins / Q000235:genetics; D016372:RNA, Antisense; D012333:RNA, Messenger / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology / Q000201:enzymology; D000098682:Steroidogenic Acute Regulatory Protein
D004041:Dietary Fats; D010750:Phosphoproteins; D016372:RNA, Antisense; D012333:RNA, Messenger; D000098682:Steroidogenic Acute Regulatory Protein; C066498:Akr1b7 protein, mouse; D000449:Aldehyde Reductase; D002786:Cholesterol Side-Chain Cleavage Enzyme