title: Proteomic approach for identification and characterization of novel immunostimulatory proteins from soluble antigens of Leishmania donovani promastigotes.
abstract: Visceral leishmaniasis (VL) caused by Leishmania donovani is a major parasitic disease prevalent in endemic regions of Bihar in India. In the absence of good chemotherapeutic options, there is a need to develop an effective vaccine against VL which should be dependent on the generation of a T helper type 1 (Th1) immune response. We have shown that soluble proteins from promastigote of a new clinical isolate of L. donovani (2001) ranging from 68 to 97.4 kDa (F2 fraction), induce Th1 responses in the peripheral blood mononuclear cells of cured Leishmania patients and hamsters and also showed significant prophylactic potential. To understand the nature of F2 proteins, it was further characterized using 2-DE, MALDI-TOF and MALDI-TOF/TOF-MS. In all, 63 spots were cut from a CBB stained gel for analysis and data was retrieved for 52 spots. A total of 33 proteins were identified including six hypothetical/unknown proteins. Major immunostimulatory proteins were identified as elongation factor-2, p45, heat shock protein (HSP)70, HSP83, aldolase, enolase, triosephosphate isomerase, protein disulfideisomerase and calreticulin. This study substantiates the usefulness of proteomics in characterizing a complex protein fraction (F2) map of soluble L. donovani promastigote antigen identified as Th1 stimulatory for its potential as vaccine targets against VL.
authors.affiliation: Division of Parasitology, Central Drug Research Institute, Lucknow, India.
authors.forename: Shraddha Kumari
authors.initials: SK
authors.lastname: Gupta
authors.forename: Brijesh Singh
authors.initials: BS
authors.lastname: Sisodia
authors.forename: Sudhir
authors.initials: S
authors.lastname: Sinha
authors.forename: Krishnan
authors.initials: K
authors.lastname: Hajela
authors.forename: Sita
authors.initials: S
authors.lastname: Naik
authors.forename: Ajit Kumar
authors.initials: AK
authors.lastname: Shasany
authors.forename: Anuradha
authors.initials: A
authors.lastname: Dube
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000737:chemistry / Q000276:immunology*; D006224:Cricetinae; D006801:Humans; D007893:Leishmania donovani / Q000737:chemistry / Q000276:immunology*; D020543:Proteome / Q000737:chemistry / Q000276:immunology*; D040901:Proteomics
chemical_list: D000953:Antigens, Protozoan; D020543:Proteome