[Determination of urinary cotinine and 1-hydroxypyrene and blood carboxyhemoglobine as the biomarkers of tobacco smoke exposure].
The following biomarkers: cotinine, 1-hydroxypyrene (1-OHP) and carboxyhemoglobin (HbCO) were used for the estimation of the tobacco smoke exposure. In our study urine and blood sampled from 98 volunteers were subjected to assessment of the biomarkers concentrations under investigation. According to the self-reports of the volunteers, all of the volunteers were divided into three groups: non-smokers, passive and active smokers. The mean values of the biomarker concentrations were calculated for these groups. In order to verify the tobacco smoke exposure specified by volunteers, we tested the real urinary cotinine amount. Based on obtained values of this biomarker, the another volunteer's partition into the groups of nonsmokers, passive and active smokers were done. The new mean values for all of these groups were calculated. The mean values obtained for both group partitions differed. The latter partition gave opportunity to use 1-OHP for the estimation of the tobacco smoke exposure not only for the active smokers but also for the passive smokers. When HbCO was taken into consideration as the biomarker, the estimation of the tobacco smoke exposure of the passive smokers was not significant different, as compared with the group of nonsmokers. The analysis of the correlation between the concentrations of 1-OHP and cotinine gave opportunity for the estimation to tobacco smoke exposure of the passive and active smokers. The estimation of the correlation, between the HbCO and cotinine concentrations was restricted for this purpose.
Zielińska-Danch
Wioleta
W
Zakład Chemii Ogólnej i Nieorganicznej, Katedra Chemii Ogólnej i Analitycznej, Slaskiej Akademii Medycznej w Katowicach. wzdanch@slam.katowice.pl
Wardas
Władysław
W
Sobczak
Andrzej
A
D000328:Adult; D015415:Biomarkers / Q000032:analysis; D002263:Carboxyhemoglobin / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003367:Cotinine / Q000652:urine*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011721:Pyrenes / Q000032:analysis*; D012680:Sensitivity and Specificity; D012907:Smoking / Q000097:blood* / Q000652:urine*; D014028:Tobacco Smoke Pollution / Q000032:analysis*
D015415:Biomarkers; D011721:Pyrenes; D014028:Tobacco Smoke Pollution; D002263:Carboxyhemoglobin; D003367:Cotinine; C033146:1-hydroxypyrene