title: [Hairy roots induction of Phellodendron chinense and production of its active constituents].
abstract: OBJECTIVE
  To introduce the hairy roots of Phellodendron chinense and determine the content of its active constituents.
  
  
  METHODS
  Transformed hairy roots of P. chinense were obtained by the transformation of Agrobacterium rhizogenes A4, R1600, ATCC15834 and R1000.
  
  
  CONCLUSIONS
  It was clearly demonstrated that T-DNA of A. rhizogenes Ri plasmid was integrated into the cells of hairy roots by PCR. The content of berberine hydrochlodride, which was determined by HPLC, was higher in hairy roots than that in the axenic plantet and callus.
authors.affiliation: Department of Biological Engineering, Chengdu University, Chengdu 610106, China.
authors.forename: Yue-hua
authors.initials: YH
authors.lastname: Wang
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Gou
authors.forename: Jie
authors.initials: J
authors.lastname: Wu
authors.forename: Wen-zhao
authors.initials: WZ
authors.lastname: Yan
authors.forename: Xian-jun
authors.initials: XJ
authors.lastname: Ma
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
mesh_terms: D001599:Berberine / Q000032:analysis / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D046508:Culture Techniques / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics; D032102:Phellodendron / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D010946:Plants, Medicinal / Q000235:genetics / Q000378:metabolism*; D012231:Rhizobium / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D004269:DNA, Bacterial; C036483:T-DNA; D001599:Berberine