Cardiac accumulation of bone marrow mononuclear progenitor cells after intracoronary or intravenous injection in pigs subjected to acute myocardial infarction with subsequent reperfusion.
OBJECTIVE
The purpose of the present study was to compare the efficacy of intracoronary and intravenous injection of autologous progenitor cells for homing to the acutely infarcted but reperfused myocardium in pigs.


METHODS
Myocardial infarction was induced in 11 anesthetized pigs by 60-min balloon inflation in the mid LAD. After balloon deflation, reperfusion was verified and autologous CD31(+) progenitor cells, or bone marrow mononuclear cells, labeled with PKH67, were injected either intracoronarily (n=6) or intravenously (n=3). By autopsy, 4-5 days after induction of infarction, tissue from the heart and other organs was obtained for fluorescence microscopy.


RESULTS
In the heart, PKH(+) cells were detected throughout the reperfused infarcted myocardium, and the number of PKH(+) cells was significantly higher after intracoronary than after intravenous injection (3.2+/-0.55 vs. 0.33+/-0.17 cells/high-power field/10(6) cells injected, P=.01). Few PKH(+) cells were detected in the spleen, lung, mesenteric lymph node, and bone marrow. In an additional animal with a coil placed in the mid LAD, progenitor cells were not detected in the infarcted myocardium or in the normal myocardium.


CONCLUSIONS
Autologous mononuclear and CD31(+) cells from bone marrow accumulated in the infarcted myocardium when injected intracoronarily or intravenously after established reperfusion, and the accumulation of cells was significantly greater after intracoronary injection than after intravenous injection. Accumulation of PKH(+) cells did not appear in the normal myocardium or in the nonreperfused infarcted myocardium. PKH(+) cells were detected in spleen, lung, and bone marrow but to a lesser degree than in the infarcted myocardium.
Grøgaard
Haakon K
HK
Institute for Experimental Medical Research (IEMR), Ulleval University Hospital, N-0407 Oslo, Norway. h.k.grogaard@klinmed.uio.no
Sigurjonsson
Olafur E
OE
Brekke
Magne
M
Kløw
Nils E
NE
Landsverk
Kirsti Solberg
KS
Lyberg
Torstein
T
Eriksen
Morten
M
Egeland
Torstein
T
Ilebekk
Arnfinn
A
D000818:Animals; D001854:Bone Marrow Cells / Q000737:chemistry / Q000166:cytology* / Q000502:physiology; D016026:Bone Marrow Transplantation / Q000379:methods*; D006321:Heart / Q000502:physiology*; D007269:Injections, Intra-Arterial; D007275:Injections, Intravenous; D009203:Myocardial Infarction / Q000601:surgery*; D015425:Myocardial Reperfusion; D009206:Myocardium / Q000166:cytology; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000737:chemistry / Q000166:cytology* / Q000502:physiology; D013552:Swine; D016896:Treatment Outcome
D019408:Platelet Endothelial Cell Adhesion Molecule-1