title: Antichlamydial antibodies and citric acid in patients with chronic prostatitis.
abstract: BACKGROUND
  The aim of the study was to evaluate the correlation between the presence of anti-C. trachomatis (C.t.) antibodies in serum and expressed prostatic secretions (EPS) and the concentration of citric acid in patients with chronic prostatitis.
  
  
  METHODS
  The study involved 34 men with chronic prostatitis. The leukocyte count, presence of anti-C.t. antibodies (IgA, IgG), and citric acid concentration were determined in the EPS. The serum was examined for IgM, IgA, and IgG anti-C.t. antibodies. Specific antibodies were determined using the EIA method. The concentration of citric acid was measured using the ultraviolet method.
  
  
  RESULTS
  Inflammation of the prostate (210 PMN) was found in 61.8% of the patients. A reduction in citric acid concentration in the EPS was detected in 58.8% of the men. Specific serum antibodies were detected in 58.8% of the patients, including 23.5% with IgM, 32.4% with IgA, and 44.1% with IgG. In all patients, serum IgM and IgA antibody titers were low, while those of IgG antibodies were strongly positive in 46.7% of the patients. Anti-C.t. antibodies in the EPS were detected in 44.1% of the patients, including 32.4% with IgA and 35.3% with IgG. In contrast to serum, the titers of IgG antibodies in the EPS were low in all the patients, while those of IgA were strongly positive in 54.5% of cases. In patients with positive serological outcomes, 85% had reduced concentrations of citric acid.
  
  
  CONCLUSIONS
  The occurrence of anti-C.t. antibodies is usually accompanied by a decrease in the concentration of citric acid in the prostatic secretion.
authors.affiliation: Center for Sexually Transmitted Disease Research and Diagnostics, Białystok, Poland centstd@poczta.onet.pl
authors.forename: Iwona
authors.initials: I
authors.lastname: Ostaszewska-Puchalska
authors.forename: Bozena
authors.initials: B
authors.lastname: Zdrodowska-Stefanow
authors.forename: Jerzy
authors.initials: J
authors.lastname: Badyda
authors.forename: Zofia
authors.initials: Z
authors.lastname: Galewska
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000097:blood; D002692:Chlamydia trachomatis / Q000276:immunology*; D002908:Chronic Disease; D019343:Citric Acid / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011467:Prostate / Q000276:immunology / Q000378:metabolism; D011472:Prostatitis / Q000097:blood / Q000276:immunology* / Q000382:microbiology*
chemical_list: D000907:Antibodies, Bacterial; D019343:Citric Acid