TopBP1 regulates human papillomavirus type 16 E2 interaction with chromatin.
Human papillomavirus type 16 (HPV16) E2 regulates transcription from and replication of the viral genome, in association with viral and cellular factors. HPV16 E2 interacts functionally with TopBP1, a cellular protein essential for the initiation of cellular, and potentially viral, DNA replication. This report demonstrates that the absence of TopBP1 results in the redistribution of HPV16 E2 into an alternative cellular protein complex, resulting in enhanced affinity for chromatin. This redistribution does not significantly alter the ability of HPV16 E2 to either activate or repress transcription. We also show colocalization of both proteins on chromatin at late stages of mitosis, suggesting that TopBP1 could be the mitotic chromatin receptor for HPV16 E2. The possible significance of the results for the regulation of the viral life cycle is discussed.
Donaldson
Mary M
MM
Institute of Comparative Medicine, Division of Pathological Sciences, University of Glasgow Faculty of Veterinary Medicine, Bearsden Road, Glasgow, G61 1QH Scotland.
Boner
Winifred
W
Morgan
Iain M
IM
D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D002843:Chromatin / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D020868:Gene Silencing; D052162:Human papillomavirus 16 / Q000378:metabolism*; D006801:Humans; D018613:Microscopy, Confocal; D009687:Nuclear Proteins / Q000378:metabolism*; D009856:Oncogene Proteins, Viral / Q000378:metabolism*; D011485:Protein Binding; D014158:Transcription, Genetic
D002352:Carrier Proteins; D002843:Chromatin; D004268:DNA-Binding Proteins; C054855:E2 protein, Human papillomavirus type 16; D009687:Nuclear Proteins; D009856:Oncogene Proteins, Viral; C110513:TOPBP1 protein, human