title: [Capillary blood flow as an index of the therapeutic effect of folinic acid in ischemia-reperfusion syndrome].
abstract: OBJECTIVE
  An intestinal reperfusion study with two aims: a) to assess the usefulness of intestinal capillary blood flow measurement by laser-Doppler for intestinal reperfusion studies; and b) to compare the effects of racemic and levo forms of folinic acid in treating the syndrome.
  
  
  METHODS
  A murine model of intestinal ischemia by completely clamping the superior mesenteric artery for 90 minutes. A comparison was made of three treatment groups: saline, folinic acid, and levo-folinic acid. The following factors were analyzed: changes in biochemical parameters (levels of creatine kinase, lactic dehydrogenase, and alkaline phosphatase at 60 minutes, and at two and seven days after restoring blood flow), capillary flow in the jejunum and ileum by laser-Doppler (during ischemia and after the first hour of reperfusion), intestinal mucosa injury, and survival curve.
  
  
  RESULTS
  Laser-Doppler provided reliable data on how the different treatments affected capillary flow during intestinal reperfusion. Levo-folinic acid improved capillary flow in the ileum after 25 minutes of reperfusion, and also reduced mucosal injury in the same stretch of intestine by the seventh day post-reperfusion (p<0.05). On the other hand, it produced an initial increase in serum enzymes during reperfusion, and did not modify survival.
  
  
  CONCLUSIONS
  The changes observed in intestinal capillary blood flow measurement by laser-Doppler have similarities with the effects of drugs on pathological mucosal changes. We could not prove that the levo form of folinic acid has a stronger protective effect versus racemic folinic acid in intestinal ischemia-reperfusion syndrome.
authors.affiliation: Laboratorio de Cirugía Experimental, Facultad de Medicina y Odontología, Universidad del País Vasco, Vizcaya, Spain.
authors.forename: J
authors.initials: J
authors.lastname: San Cristóbal
authors.forename: I
authors.initials: I
authors.lastname: Cearra
authors.forename: B
authors.initials: B
authors.lastname: Otero
authors.forename: T
authors.initials: T
authors.lastname: Martínez-Astorquiza
authors.forename: H
authors.initials: H
authors.lastname: Marín
authors.forename: I
authors.initials: I
authors.lastname: García-Alonso
mesh_terms: D000818:Animals; D002196:Capillaries; D007422:Intestines / Q000098:blood supply*; D002955:Leucovorin / Q000627:therapeutic use*; D008297:Male; D051381:Rats; D012039:Regional Blood Flow; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000201:enzymology
chemical_list: D002955:Leucovorin