title: Seasonal change in osteoid thickness and mineralization lag time in ambulant patients.
abstract: Low vitamin D levels are common. Bone biopsies taken from 121 ambulant patients were therefore reviewed. Seasonal changes in mineralization correlated inversely with serum 25-hydroxyvitamin D but not the more active metabolite, 1,25-dihydroxyvitamin D. This implies that the latter is produced in bone.
  
  
  BACKGROUND
  It has been 30 yr since a seasonal variation in osteoid surfaces and calcification fronts was noted in bone biopsies from hip fracture patients in Leeds and attributed to vitamin D status. It was suggested at that time that mild vitamin D deficiency might cause osteoporosis from malabsorption of calcium and more severe deficiency osteomalacia, but little has been published on this subject since.
  
  
  METHODS
  We examined bone biopsies, calcium absorption data, and serum vitamin D metabolites in 121 patients attending our osteoporosis clinics in Adelaide. Biopsies were collected from the anterior iliac crest with a Jamshidi needle after two stat oral doses of 1 g of tetracycline 10 days apart, processed into plastic without demineralization, and all parameters were measured by point counting using a Weibel II graticule. Calcium absorption was measured after an oral dose of 5 microCi of (45)Ca in 250 ml of water with 20 mg of calcium carrier. Serum 25-hydroxyvitamin D [25(OH)D] was measured by radioimmunoassay and 1,25-dihydroxyvitamin D [1,25(OH)(2)D] by radioimmunoassay after high-performance liquid chromatography (HPLC).
  
  
  RESULTS
  25(OH)D levels were lower from late autumn to early spring (April to September) than from late spring to early autumn (October to March) (51 +/- 23 versus 61 +/- 27 [SD] nM; p=0.040). None of the biopsies yielded a diagnosis of osteomalacia, but osteoid thickness (O.Th.) was greater in the winter than the summer months (8.5 +/- 3.6 versus 7.1 +/- 2.8 microm; p=0.015) as was mineralization lag time (MLT; 11.9 +/- 5.2 versus 9.5 +/- 3.6; p=0.005). O.Th and log MLT were both inversely related to serum 25(OH)D (p=0.014 and 0.036) but not serum 1,25(OH)(2)D. Calcium absorption was related to serum 1,25(OH)(2)D but not serum 25(OH)D.
  
  
  CONCLUSIONS
  We conclude that circulating 25(OH)D affects the mineralization process, whereas circulating 1,25(OH)(2)D affects bone indirectly through its effect on calcium absorption.
authors.affiliation: Institute of Medical and Veterinary Science, Adelaide, South Australia, Australia. allan.need@imvs.sa.gov.au
authors.forename: Allan G
authors.initials: AG
authors.lastname: Need
authors.forename: Michael
authors.initials: M
authors.lastname: Horowitz
authors.forename: Howard A
authors.initials: HA
authors.lastname: Morris
authors.forename: Robert
authors.initials: R
authors.lastname: Moore
authors.forename: Christopher
authors.initials: C
authors.lastname: Nordin
mesh_terms: D000042:Absorption; D000284:Administration, Oral; D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000008:administration & dosage; D001315:Australia; D015519:Bone Density / Q000187:drug effects; D002113:Calcification, Physiologic / Q000187:drug effects; D002117:Calcitriol / Q000097:blood* / Q000172:deficiency; D002118:Calcium / Q000008:administration & dosage / Q000378:metabolism; D005260:Female; D006801:Humans; D007085:Ilium / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D010024:Osteoporosis / Q000097:blood* / Q000188:drug therapy / Q000503:physiopathology; D010045:Outpatients; D012621:Seasons; D013752:Tetracycline / Q000008:administration & dosage; D044465:White People
chemical_list: D000900:Anti-Bacterial Agents; D013752:Tetracycline; D002117:Calcitriol; D002118:Calcium