title: The construction of a Solanum habrochaites LYC4 introgression line population and the identification of QTLs for resistance to Botrytis cinerea.
abstract: Tomato (Solanum lycopersicum) is susceptible to grey mold (Botrytis cinerea). Partial resistance to this fungus has been identified in accessions of wild relatives of tomato such as Solanum habrochaites LYC4. In a previous F(2) mapping study, three QTLs conferring resistance to B. cinerea (Rbcq1, Rbcq2 and Rbcq4a) were identified. As it was probable that this study had not identified all QTLs involved in resistance we developed an introgression line (IL) population (n = 30), each containing a S. habrochaites introgression in the S. lycopersicum cv. Moneymaker genetic background. On average each IL contained 5.2% of the S. habrochaites genome and together the lines provide an estimated coverage of 95%. The level of susceptibility to B. cinerea for each of the ILs was assessed in a greenhouse trial and compared to the susceptible parent S. lycopersicum cv. Moneymaker. The effect of the three previously identified loci could be confirmed and seven additional loci were detected. Some ILs contains multiple QTLs and the increased resistance to B. cinerea in these ILs is in line with a completely additive model. We conclude that this set of QTLs offers good perspectives for breeding of B. cinerea resistant cultivars and that screening an IL population is more sensitive for detection of QTLs conferring resistance to B. cinerea than the analysis in an F(2) population.
authors.affiliation: Graduate School Experimental Plant Sciences, Laboratory of Plant Breeding, Wageningen University, PO Box 386, 6700 AJ Wageningen, The Netherlands. Richard.Finkers@wur.nl
authors.forename: Richard
authors.initials: R
authors.lastname: Finkers
authors.forename: Adriaan W
authors.initials: AW
authors.lastname: van Heusden
authors.forename: Fien
authors.initials: F
authors.lastname: Meijer-Dekens
authors.forename: Jan A L
authors.initials: JA
authors.lastname: van Kan
authors.forename: Paul
authors.initials: P
authors.lastname: Maris
authors.forename: Pim
authors.initials: P
authors.lastname: Lindhout
mesh_terms: D020171:Botrytis / Q000145:classification / Q000472:pathogenicity*; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D003433:Crosses, Genetic; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D005819:Genetic Markers; D005828:Genetics, Population; D018745:Genome, Plant; D006579:Heterozygote; D006720:Homozygote; D007113:Immunity, Innate / Q000235:genetics*; D008957:Models, Genetic; D021141:Nucleic Acid Amplification Techniques; D011110:Polymorphism, Genetic; D040641:Quantitative Trait Loci; D011995:Recombination, Genetic; D012639:Seeds / Q000235:genetics; D012984:Software; D032322:Solanum / Q000145:classification / Q000235:genetics* / Q000276:immunology*
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers