[Effects of grape seed proanthocyanidins extracts on experimental diabetic nephropathy in rats].
OBJECTIVE
To study the effects of grape seed proanthocyanidins extracts(GSPE) and its mechanism on early renal lesions of diabetic rats.


METHODS
Diabetic rats induced by alloxan were given GSPE intragastrically for 6 weeks, then the antioxidative indexes and NO content, NOS activity in kidney and serum were measured, and the renal function indexes were tested as well.


RESULTS
Compared with the diabetic group, the urinary protein/24h, levels of blood urea nitrogen(BUN)and serum creatinine(SCr), creatinine clearance rate(CCr) and the ratio of kidney weight/body weight were decreased, the SOD activity in kidney was raised while MDA content was fall in the GSPE group(high dose), and the differences were all significant. The NO content in the kidney and NOS activity in kidney and serum decreased in the GSPE (low dose)group, and there was significant difference when compared with diabetic group( P <0.05) .


CONCLUSIONS
GSPE has the effect in protecting kidney of diabetic rats, the mechanism might be related with its action in increasing the renal antioxidative ability,decreasing the content of NO and the activity of NOS in kidney and serum.
Liu
Yan-ni
YN
Department of Nutrition and Food Hygiene, School of Public Health, Fudan University, Shanghai 200032, China.
Shen
Xin-nan
XN
Yao
Guo-ying
GY
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000378:metabolism; D003928:Diabetic Nephropathies / Q000517:prevention & control*; D007668:Kidney / Q000378:metabolism; D007677:Kidney Function Tests; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D044945:Proanthocyanidins / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012639:Seeds / Q000737:chemistry; D027843:Vitis / Q000737:chemistry*
D000975:Antioxidants; D010936:Plant Extracts; D044945:Proanthocyanidins; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase