Effect of Cassia fistula Linn. leaf extract on diethylnitrosamine induced hepatic injury in rats.
The hepatoprotective and antioxidant effect of Cassia fistula Linn. leaf extract on liver injury induced by diethylnitrosamine (DEN) was investigated. Wistar rats weighing 200+/-10g were administered a single dose of DEN (200mg/kg b.w., i.p.) and left for 30 days. For hepatoprotective studies, ethanolic leaf extract (ELE) of C. fistula Linn. (500mg/kg b.w., p.o.) was administered daily for 30 days. AST, ALT, ALP, LDH, gamma-GT and bilirubin were estimated in serum and liver tissue. Lipid peroxidation (LPO), SOD and CAT were also estimated in liver tissue as markers of oxidative stress. DEN induced hepatotoxicity in all the treated animals were evident by elevated serum ALT, AST, ALP and bilirubin levels and a simultaneous fall in their levels in the liver tissue after 30 days. Induction of oxidative stress in the liver was evidenced by increased LPO and fall in the activities of SOD and CAT. ELE administration for 30 days prevented the DEN induced hepatic injury and oxidative stress. In conclusion, it was observed that ELE of C. fistula Linn. protects the liver against DEN induced hepatic injury in rats.
Pradeep
Kannampalli
K
Department of Pharmacology and Environmental Toxicology, Dr. A.L.M. P.G. Institute of Basic Medical Science, University of Madras, Taramani, Chennai 600113, India. kannampallipradeep@rediffmail.com
Mohan
Chandrasekaran Victor Raj
CV
Gobianand
Kuppannan
K
Karthikeyan
Sivanesan
S
D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000477:Alkylating Agents; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D002366:Cassia / Q000737:chemistry*; D002374:Catalase / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury; D004052:Diethylnitrosamine; D000431:Ethanol / Q000737:chemistry; D007770:L-Lactate Dehydrogenase / Q000097:blood; D015227:Lipid Peroxidation / Q000187:drug effects; D008107:Liver Diseases / Q000378:metabolism / Q000517:prevention & control*; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D005723:gamma-Glutamyltransferase / Q000097:blood
D000477:Alkylating Agents; D000975:Antioxidants; D010936:Plant Extracts; D004052:Diethylnitrosamine; D000431:Ethanol; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D013482:Superoxide Dismutase; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D001663:Bilirubin