title: Immuno-electron microscopic localisation of caveolin 1 in human placenta.
abstract: We have localised the placental endothelial marker caveolin-1 at the ultrastructural level using indirect immunogold labelling. The particulate label has been quantified to assess the distribution of the target protein within term placental chorionic villi. The mesodermal compartment of the tissue was more heavily labelled than the ectodermally derived trophoblast. Basal plate lining endothelium and villous endothelium had similar immunoreactivity with anti-caveolin-1 antibody. A polarised distribution of the caveolin within chorionic villous capillary endothelial cells was observed. As evidenced by immuno-reactivity, the protein was statistically significantly more concentrated in the region associated with the basal membrane than the apical membrane. The latter region contained in turn significantly more anti-caveolin-1 immunoreactivity than the central region. These differences are discussed in the light of possible transport and signalling platform rôles for villous and basal plate endothelium.
authors.affiliation: Laboratory for Developmental Cell Sciences, Department of Infection Immunity and Inflammation, School of Medicine and Biological Sciences, University of Leicester and Warwick Medical Schools, University Road, Leicester LE1 9HN, UK.
authors.forename: Simon
authors.initials: S
authors.lastname: Byrne
authors.forename: John
authors.initials: J
authors.lastname: Ahenkorah
authors.forename: Bismarck
authors.initials: B
authors.lastname: Hottor
authors.forename: C
authors.initials: C
authors.lastname: Lockwood
authors.forename: Colin D
authors.initials: CD
authors.lastname: Ockleford
mesh_terms: D051242:Caveolin 1 / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D004730:Endothelium, Vascular / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D005347:Fibroblasts / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D016253:Microscopy, Immunoelectron; D010920:Placenta / Q000378:metabolism* / Q000648:ultrastructure*; D011247:Pregnancy
chemical_list: D051242:Caveolin 1