Improved polymerase chain reaction technique for determining the species composition of Eimeria in poultry litter.
An improved polymerase chain reaction (PCR)-based method for determining the species composition of Eimeria in poultry litter was developed by incorporating species-specific internal standards in the assay. Internal standard molecules were prepared by fusing seven different Eimeria species-specific intervening transcribed sequence 1 (ITS1) rDNA primer pairs to a non-Eimeria DNA molecule and by cloning the hybrid DNA molecules into a plasmid. The internal DNA standards were then used in Eimeria-specific ITS 1 PCR, and they were found to be capable of detecting E. acervulina, E. maxima, E. praecox, and E. tenella oocysts isolated directly from poultry litter.
Jenkins
M C
MC
Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, ARS, USDA, Beltsville, MD 20705, USA.
Miska
K
K
Klopp
S
S
D000818:Animals; D002645:Chickens / Q000469:parasitology*; D021901:DNA, Intergenic / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics; D004539:Eimeria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D005430:Floors and Floorcoverings; D006799:Housing, Animal; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity
D021901:DNA, Intergenic; D016054:DNA, Protozoan