Neurotoxic activity of Gln49 phospholipase A(2) from Gloydius ussuriensis snake venom.
A novel neurotoxic protein phospholipase A(2) (PLA(2)), molecular weight 13 881.83 Da, has been isolated from snake venom of Gloydius ussuriensis, named as Gln49-PLA(2), which shows weak lethal toxic, myotoxic and apparent anticoagulant activity, but lacks phospholipase activity. The Gln49-PLA(2) obviously induced an increase of the pain threshold in intoxicated 615 mice compared with the control group, suggesting it is a neurotoxin. Hot-plate tests also showed that its analgesic activity was dose-dependent, and naloxone antagonized the analgesic effect, implying the mechanism of action of Gln49-sPLA(2) is correlated with opioid receptors. Electrophysiology studies revealed decreases in the action potential and the nerve conduction velocity in isolated hoptoad (Bufo bufo gargarizans Cantor) sciatic nerve, indicating Gln49-PLA(2) most probably had effects on ion channels.
Chang
Yan
Y
Department of Bioscience and Biotechnology, Dalian University of Technology, 2 Linggong road, Dalian 116024, P. R. China.
Li
Yingxue
Y
Bao
Yongming
Y
An
Lijia
L
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D003435:Crotalid Venoms / Q000633:toxicity*; D005260:Female; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D009270:Naloxone / Q000494:pharmacology; D009431:Neural Conduction / Q000187:drug effects; D009498:Neurotoxins / Q000633:toxicity*; D010741:Phospholipases A / Q000633:toxicity*
D003435:Crotalid Venoms; D009498:Neurotoxins; D009270:Naloxone; D010741:Phospholipases A