Dopamine but not 3,4-dihydroxy phenylacetic acid (DOPAC) inhibits brain respiratory chain activity by autoxidation and mitochondria catalyzed oxidation to quinone products: implications in Parkinson's disease.
This study reveals that, in contrast to dopamine (DA), 3,4 dihydroxyphenylacetic acid (DOPAC) during in vitro incubation up to 2 h causes only marginal inhibition of rat brain mitochondrial respiratory chain activity, a minimal loss of protein free thiols and very little quinoprotein adduct formation. The damaging effects of DA on brain mitochondria are, however, conspicuous and apparently mediated by quinone oxidation products generated by autoxidation of DA as well as catalyzed by a mitochondrial activity, inhibitable by clorgyline (2.5-10 microM) and cyanide (1 mM).
Jana
Sirsendu
S
Department of Biochemistry, Institute of Post Graduate Medical Education & Research, 244B, Acharya J.C. Bose Road, Kolkata-700020, India.
Maiti
Arpan Kumar
AK
Bagh
Maria Bindu
MB
Banerjee
Kalpita
K
Das
Amitabha
A
Roy
Arun
A
Chakrabarti
Sasanka
S
D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D016227:Benzoquinones / Q000378:metabolism*; D001921:Brain / Q000201:enzymology; D004298:Dopamine / Q000378:metabolism*; D004579:Electron Transport / Q000502:physiology; D045222:Electron Transport Chain Complex Proteins / Q000187:drug effects / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D010084:Oxidation-Reduction; D010300:Parkinson Disease / Q000201:enzymology / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D016227:Benzoquinones; D045222:Electron Transport Chain Complex Proteins; D015102:3,4-Dihydroxyphenylacetic Acid; C004532:quinone; D004298:Dopamine