title: Polyhedrin gene sequence and phylogenetic analysis of a nucleopolyhedrovirus isolated from Orgyia ericae Germar.
abstract: Molecular characterization of a nucleopolyhedrovirus (NPV) isolated from the diseased larva of Orgyia ericae Germar was firstly analyzed. The genomic size of O. ericae NPV was estimated to be 134.6 kb by restriction endonuclease analysis. The gene encoding the major structural protein, polyhedrin, was cloned and sequenced. Phylogenetic analyses using polyhedrin sequences revealed that O. ericae NPV (OeNPV) was a member of the Group II NPVs and was closely related to the BusuSNPV and OpSNPV cluster. Electron microscopic observations confirmed that OeNPV was a single nucleocapsid type virus (SNPV).
authors.affiliation: Institute of Insect Sciences, Zhejiang University, Hangzhou 310029, P. R. China. yangzi0224@sohu.com
authors.forename: Li-Rong
authors.initials: LR
authors.lastname: Yang
authors.forename: Dun
authors.initials: D
authors.lastname: Wang
authors.forename: Li-Qing
authors.initials: LQ
authors.lastname: Duan
authors.forename: Chuan-Xi
authors.initials: CX
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D007814:Larva / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D009036:Moths / Q000254:growth & development / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D000077183:Occlusion Body Matrix Proteins; D010802:Phylogeny; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000145:classification* / Q000235:genetics*; D015678:Viral Structural Proteins
chemical_list: D000077183:Occlusion Body Matrix Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; C009829:polyhedrin protein, Nucleopolyhedrovirus