Coupling permanganate oxidation with microbial dechlorination of tetrachloroethene.
For sites contaminated with chloroethene non-aqueous-phase liquids, designing a remediation system that couples in situ chemical oxidation (ISCO) with potassium permanganate (KMnO4) and microbial dechlorination may be complicated because of the potentially adverse effects of ISCO on anaerobic bioremediation processes. Therefore, one-dimensional column studies were conducted to understand the effect of permanganate oxidation on tetrachloroethene (PCE) dechlorination by the anaerobic mixed culture KB-1. Following the confirmation of PCE dechlorination, KMnO4 was applied to all columns at a range of concentrations and application velocities to simulate varied distances from oxidant injection. Immediately following oxidation, reductive dechlorination was inhibited; however, after passing several pore volumes of sterile growth medium through the columns after oxidation, a rebound of PCE dechlorination activity was observed in every inoculated column without the need to reinoculate. The volume of medium required for a rebound of dechlorination activity differed from 1.1 to 8.1 pore volumes (at a groundwater velocity of 4 cm/d), depending on the specific condition of oxidant application.
Sahl
Jason W
JW
Colorado School of Mines/ESE division, Golden, CO 80401, USA. jsahl@mines.edu
Munakata-Marr
Junko
J
Crimi
Michelle L
ML
Siegrist
Robert L
RL
D001673:Biodegradation, Environmental; D041862:Chloroflexi / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D003470:Culture Media; D010084:Oxidation-Reduction; D011196:Potassium Permanganate / Q000737:chemistry* / Q000494:pharmacology; D012989:Soil Pollutants / Q000737:chemistry* / Q000378:metabolism; D013750:Tetrachloroethylene / Q000737:chemistry* / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000737:chemistry* / Q000378:metabolism; D018508:Water Purification
D003470:Culture Media; D012989:Soil Pollutants; D014874:Water Pollutants, Chemical; D011196:Potassium Permanganate; D013750:Tetrachloroethylene