In vivo adaptation of the Wayne model of latent tuberculosis.
Cultures of Mycobacterium tuberculosis grown under oxygen depletion conditions enter into a state of nonreplicating persistence that may reflect a physiologically latent state. When these cultures were harvested and injected intranasally into mice, no bacteria could be recovered from the lungs for about 3 weeks, but after that evidence of regrowth was observed. Preimmunization of mice with a panel of selected vaccine candidates slowed or prevented this event. This simple model has potential for identifying vaccines targeting latent tuberculosis.
Woolhiser
Lisa
L
Mycobacteria Research Laboratories, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA.
Tamayo
Marcela Henao
MH
Wang
Baolin
B
Gruppo
Veronica
V
Belisle
John T
JT
Lenaerts
Anne J
AJ
Basaraba
Randall J
RJ
Orme
Ian M
IM
D000222:Adaptation, Physiological; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D008168:Lung / Q000382:microbiology; D051379:Mice; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis / Q000254:growth & development* / Q000378:metabolism / Q000472:pathogenicity* / Q000502:physiology; D010100:Oxygen / Q000494:pharmacology*; D013047:Specific Pathogen-Free Organisms; D014397:Tuberculosis, Pulmonary / Q000382:microbiology / Q000473:pathology*
D010100:Oxygen