title: EST-SSR DNA polymorphism in durum wheat (Triticum durum L.) collections.
abstract: SSRs derived from EST were molecular markers belonging to the transcribed region of the genome. Therefore, any polymorphism detected using EST-SSRs might reflect the better relationship among species or varieties. Using wheat EST-SSR markers, 60 durum wheat (Triticum durum L.) accessions from seven countries were investigated. Twenty-five primer pairs could amplify successfully in the 60 durum wheat accessions, of which tri-nucleotide repeats were the dominant type, and revealed 26 loci on all seven wheat homologous chromosome groups. A total of 87 eSSR alleles were detected, and the number of alleles detected by a single pair of primers ranged from 1 to 11, with an average of 3.3 alleles per locus. Higher numbers of alleles and PIC were identified on the B genome than those on the A genome.
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Ya'an, Sichuan China.
authors.forename: Han-Yan
authors.initials: HY
authors.lastname: Wang
authors.forename: Yu-Ming
authors.initials: YM
authors.lastname: Wei
authors.forename: Ze-Hong
authors.initials: ZH
authors.lastname: Yan
authors.forename: You-Liang
authors.initials: YL
authors.lastname: Zheng
mesh_terms: D000483:Alleles; D032461:Chromosomes, Plant / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D020224:Expressed Sequence Tags; D018745:Genome, Plant; D018895:Microsatellite Repeats; D010802:Phylogeny; D011110:Polymorphism, Genetic; D014908:Triticum / Q000145:classification / Q000235:genetics*
chemical_list: D018744:DNA, Plant