Suppression of behavioural and physiological oestrus in the mare by vaccination against GnRH.
OBJECTIVE
To examine the immunogenicity of an equine immunocontraceptive vaccine and its efficacy in controlling hormone-related behaviour.


METHODS
A total of 24 mares at two sites in Australia were vaccinated with an immunocontraceptive vaccine comprising gonadotrophin releasing hormone (GnRH) conjugated to a carrier protein in immunostimulating complex as an adjuvant. Twelve animals at each site received a placebo of adjuvant alone and served as controls for seasonal oestrus, hormonal and behaviour patterns. Animals were observed for injection site reactions, ovarian and follicular activity, and serum levels of antibody, 17beta-oestradiol and progesterone in the weeks following vaccination. Mares were also examined for oestrous behaviour by teasing with a stallion.


RESULTS
All mares responded to vaccination. Two weeks following the second vaccination there was a peak in antibody response to GnRH that declined gradually over the following weeks. Commensurate with the elevated anti-GnRH antibody there was a marked effect on ovarian activity with a reduction in 17beta-oestradiol and progesterone levels in the 24 vaccinated mares. There was also a reduction of oestrus-related behaviour as determined by a teaser stallion. This effect lasted a minimum of 3 months and correlated with the initial level of antibody response.


CONCLUSIONS
Following a conventional two-dose immunisation regime this commercially available equine immunocontraceptive vaccine was effective at inhibiting oestrous behaviour for at least 3 months. This vaccine has a high level of safety since there were no significant local reactions nor were there any adverse systemic responses to vaccination.
Elhay
M
M
Veterinary Medicines Research and Development, Pfizer Animal Health, Parkville, VIC 3052. Martin.Elhay@pfizer.com
Newbold
A
A
Britton
A
A
Turley
P
P
Dowsett
K
K
Walker
J
J
D000818:Animals; D000906:Antibodies / Q000097:blood; D004958:Estradiol / Q000097:blood; D004971:Estrus / Q000187:drug effects / Q000502:physiology; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000276:immunology* / Q000494:pharmacology*; D006736:Horses / Q000502:physiology*; D007986:Luteinizing Hormone / Q000097:blood; D010060:Ovulation / Q000187:drug effects* / Q000502:physiology; D011374:Progesterone / Q000097:blood; D012449:Safety; D012726:Sexual Behavior, Animal / Q000187:drug effects / Q000502:physiology; D013997:Time Factors; D014611:Vaccination / Q000662:veterinary
D000906:Antibodies; D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone