title: Immunomodulatory effects of two sapogenins 1 and 2 isolated from Luffa cylindrica in Balb/C mice.
abstract: Two Triterpenoids (sapogenins 1 and 2) isolated from Luffa cylindrica were subjected to immunomodulatory activity in male Balb/c mice. Mice were treated with three doses of sapogenins 1 and 2 (10, 30 and 100 mg/kg) and levamisole (2.5 mg/kg) used as a standard reference drug for 15 days. Immune responses to T-dependent antigen SRBCs were observed using parameters like HA, PFC, DTH, lymphocyte proliferation and phagocytosis. As regards these parameters, sapogenins 1 and 2 elicited a significant increase in the HA, PFC and DTH response at dose 10 mg/kg (P<0.01) and 100 mg/kg (P<0.001), respectively. Sapogenins 1 and 2 also showed significant dose-dependent decrease and increase in lymphocyte proliferation assay and phagocytic activity of macrophages. Overall, sapogenins 1 and 2 showed dose relative immunostimulatory effect on in vivo immune functions in mice.
authors.affiliation: Division of Pharmacology, Regional Research Laboratory (CSIR), Jammu, Tawi 180001, India.
authors.forename: Anamika
authors.initials: A
authors.lastname: Khajuria
authors.forename: Amit
authors.initials: A
authors.lastname: Gupta
authors.forename: Saraswati
authors.initials: S
authors.lastname: Garai
authors.forename: Basanti Purinima
authors.initials: BP
authors.lastname: Wakhloo
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000276:immunology; D006462:Hemolytic Plaque Technique; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007155:Immunologic Factors / Q000494:pharmacology*; D029776:Luffa / Q000737:chemistry*; D008214:Lymphocytes / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008807:Mice, Inbred BALB C; D012502:Sapogenins / Q000302:isolation & purification* / Q000494:pharmacology*; D012756:Sheep / Q000276:immunology; D013329:Structure-Activity Relationship; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D007155:Immunologic Factors; D012502:Sapogenins; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue