Correlation among clinicopathological parameters of myocardial damage in rats treated with isoproterenol.
The correlation among clinicopathological parameters of myocardial damage was investigated in rats administered a single subcutaneous dose of isoproterenol at 0 (saline), 0.04, 0.4 and 4 mg/kg. Total lactate dehydrogenase (LDH), creatine kinase (CK), and their isoenzymes (LDH-1, LDH-2 and CK-MB), as well as troponin I and tropinin T, were measured 4 h after the administration of the drug. Troponin I was determined by a chemiluminescence method using Bayer Centaur and DPC Immulyze, as well as by ELISA. Troponin T was assayed semi-quantitatively using Trop T Sensitive. A high correlation was found among LDH isoenzymes, troponin I (Centaur) and troponin T. The present result provides a baseline for interpreting changes in the different parameters of myocardial damage assayed by different methods in toxicity studies.
Kurata
Masaaki
M
Worldwide Safety Sciences, Pfizer Global Research and Development, Nagoya Laboratories, Pfizer Inc., 5-2 Taketoyo, Aichi 470-2393, Japan.
Iidaka
Takeshi
T
Sasayama
Yukiko
Y
Fukushima
Tamio
T
Sakimura
Masanori
M
Shirai
Norimitsu
N
D000818:Animals; D015415:Biomarkers / Q000097:blood; D003402:Creatine Kinase / Q000097:blood*; D006321:Heart / Q000187:drug effects*; D007279:Injections, Subcutaneous; D007527:Isoenzymes / Q000097:blood; D007545:Isoproterenol / Q000008:administration & dosage / Q000633:toxicity*; D007770:L-Lactate Dehydrogenase / Q000097:blood*; D008297:Male; D009206:Myocardium / Q000473:pathology*; D051381:Rats; D011919:Rats, Inbred Strains; D018675:Toxicity Tests / Q000379:methods*; D019210:Troponin I / Q000097:blood*; D020107:Troponin T / Q000097:blood*
D015415:Biomarkers; D007527:Isoenzymes; D019210:Troponin I; D020107:Troponin T; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase; D007545:Isoproterenol