Genetic typing of equine arteritis virus isolates from Argentina.
We report the nucleotide sequence and genetic diversity of four Equine Arteritis Virus (EAV) ORF 5 and 6 from Argentina isolates, obtained from asymptomatic virus-shedding stallions. Nucleic acid recovered from the isolates were amplified by RT-PCR and sequenced. Nucleotide and deduced amino acid sequences from the Argentine isolates were compared with 17 sequences available from the GenBank. Phylogenetic analysis revealed that the Argentine isolates grouped together in a definite cluster near European strains. Despite the greater genetic variability among ORF 5 from different isolates and strains of EAV, phylogenetic trees based on ORF 5 and 6 are similar. Both trees showed that virus sequences from America and Europe segregate into distinct clades based on sequence analysis of either ORF 5 or 6. This study constitutes the first characterization of Argentine EAV isolates.
Echeverría
María Gabriela
MG
Virology, Faculty of Veterinary Sciences, National University of La Plata, 60 and 118, CC 296, 1900 La Plata, Argentina. gecheverria@fcv.unlp.edu.ar
Díaz
Silvina
S
Metz
Germán Ernesto
GE
Serena
María Soledad
MS
Panei
Carlos Javier
CJ
Nosetto
Edgardo
E
D000595:Amino Acid Sequence; D000818:Animals; D001118:Argentina; D002460:Cell Line; D007240:Equartevirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D005260:Female; D006736:Horses / Q000821:virology*; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000302:isolation & purification; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012661:Semen / Q000821:virology; D016415:Sequence Alignment
D012367:RNA, Viral