title: Rapid coupling of Surface Plasmon Resonance (SPR and SPRi) and ProteinChip based mass spectrometry for the identification of proteins in nucleoprotein interactions.
abstract: We compared coupling approaches of SPR to LC-MS and ProteinChip-based mass spectrometry (SELDI) as a means of identifying proteins captured on DNA surfaces. The approach we outline has the potential to allow multiple, quantitative analysis of macromolecular interactions followed by rapid mass spectrometry identification of retained material.
authors.affiliation: Enzymologie et cinétique structurale, Laboratoire de Biotechnologies et de Pharmacologie génétique Appliquée, UMR 8113 CNRS, Institut d'Alembert, Ecole Normale Supérieur de Cachan, Cachan, France.
authors.forename: Emeline
authors.initials: E
authors.lastname: Bouffartigues
authors.forename: Hervé
authors.initials: H
authors.lastname: Leh
authors.forename: Marielle
authors.initials: M
authors.lastname: Anger-Leroy
authors.forename: Sylvie
authors.initials: S
authors.lastname: Rimsky
authors.forename: Malcolm
authors.initials: M
authors.lastname: Buckle
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis; D002853:Chromatography, Liquid; D004268:DNA-Binding Proteins / Q000032:analysis*; D009687:Nuclear Proteins / Q000032:analysis*; D040081:Protein Array Analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C031908:H-NS protein, bacteria; D009687:Nuclear Proteins