title: Quantitative surface-enhanced resonance Raman scattering of phthalocyanine-labelled oligonucleotides.
abstract: The evaluation of phthalocyanine labels for the surface-enhanced resonance Raman scattering (SERRS) detection of oligonucleotides is reported. Three phthalocyanine-labelled oligonucleotides were assessed, each containing a different metal centre. Detection limits for each labelled oligonucleotide were determined using two excitation frequencies where possible. Limits of detection as low as 2.8 x 10(-11) mol. dm(-3) were obtained which are comparable to standard fluorescently labelled probes used in previous SERRS studies. The identification of two phthalocyanine-labelled oligonucleotides without separation was also demonstrated indicating their suitability for multiplexing. This study extends the range of labels suitable for quantitative surface-enhanced resonance Raman scattering with silver nanoparticles and offers more flexibility and choice when considering SERRS for quantitative DNA detection.
authors.affiliation: Centre for Molecular Nanometrology, WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow, UK.
authors.forename: A
authors.initials: A
authors.lastname: Macaskill
authors.forename: A A
authors.initials: AA
authors.lastname: Chernonosov
authors.forename: V V
authors.initials: VV
authors.lastname: Koval
authors.forename: E A
authors.initials: EA
authors.lastname: Lukyanets
authors.forename: O S
authors.initials: OS
authors.lastname: Fedorova
authors.forename: W E
authors.initials: WE
authors.lastname: Smith
authors.forename: K
authors.initials: K
authors.lastname: Faulds
authors.forename: D
authors.initials: D
authors.lastname: Graham
mesh_terms: D004247:DNA / Q000032:analysis; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry*; D054833:Isoindoles; D008670:Metals / Q000737:chemistry; D015345:Oligonucleotide Probes / Q000032:analysis / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D007211:Indoles; D054833:Isoindoles; D008670:Metals; D015345:Oligonucleotide Probes; D004247:DNA; C013647:phthalocyanine