UVB induced oxidative stress in human keratinocytes and protective effect of antioxidant agents.
This study aims at exploring the oxidative stress in keratinocytes induced by UVB irradiation and the protective effect of nutritional antioxidants. Cultured Colo-16 cells were exposed to UVB in vitro followed by measurement of reactive oxygen species (ROS), endogenous antioxidant enzyme activity, as well as cell death in the presence or absence of supplementation with vitamin C, vitamin E, or Ginsenoside Panoxatriol. Intracellular ROS content was found significantly reduced 1 h after exposure, but increased at later time points. After exposure to 150-600 J m(-2) UVB, reduction of ROS content was accompanied by increased activity of catalase and CuZn-superoxide dismutase at early time points. Vitamins C and E, and Ginsenoside Panoxatriol counteracted the increase of ROS in the Colo-16 cells induced by acute UVB irradiation. At the same time, Ginsenoside Panoxatriol protected the activity of CuZn-superoxide dismutase, while vitamin E showed only a moderate protective role. Vitamins C and E, and Ginsenoside Panoxatriol in combination protected the Colo-16 cells from UVB-induced apoptosis, but not necrosis. These findings suggest that vitamins C and E as well as Ginsenoside Panoxatriol are promising protective agents against UVB-induced damage in skin cells.
Jin
Guang-Hui
GH
MH Radiobiology Research Unit, Jilin University School of Public Health, 1163 Xinmin Street, Changchun, Jilin, 130021, People's Republic of China.
Liu
Yang
Y
Jin
Shun-Zi
SZ
Liu
Xiao-Dong
XD
Liu
Shu-Zheng
SZ
D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000528:radiation effects; D001205:Ascorbic Acid / Q000494:pharmacology; D002478:Cells, Cultured; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D036145:Ginsenosides / Q000494:pharmacology; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism / Q000528:radiation effects*; D018384:Oxidative Stress / Q000528:radiation effects*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D014466:Ultraviolet Rays; D014810:Vitamin E / Q000494:pharmacology
D000975:Antioxidants; D036145:Ginsenosides; D011837:Radiation-Protective Agents; D014810:Vitamin E; D001205:Ascorbic Acid