title: Degradation of DNA by bisulfite treatment.
abstract: A significant level of target degradation was caused by bisulfite treatment for methylcytosine-selective hydrolysis. The depyrimidination proceeded via addition of bisulfite to pyrimidines in DNA. The quantification with real-time PCR after conventional bisulfite treatment showed a large decrease in the amount of full-length DNA.
authors.affiliation: Frontier Research System, RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Tanaka
authors.forename: Akimitsu
authors.initials: A
authors.lastname: Okamoto
mesh_terms: D000042:Absorption; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D003596:Cytosine / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D015195:Drug Design; D044127:Epigenesis, Genetic; D015870:Gene Expression; D008956:Models, Chemical; D008968:Molecular Conformation; D016133:Polymerase Chain Reaction; D011108:Polymers / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013447:Sulfites / Q000737:chemistry*; D013997:Time Factors
chemical_list: D011108:Polymers; D013447:Sulfites; D003596:Cytosine; D004247:DNA; C042345:hydrogen sulfite