title: Dynamic changes in histone acetylation during sheep oocyte maturation.
abstract: The changes in histone acetylation are not always consistent in various cell types and at different developmental stages. We immunostained specific antibodies against acetylated lysine 9 of histone H3 and acetylated lysines 5 and 12 of histone H4 in an effort to understand the detailed changes in histone acetylation during sheep oocyte meiosis. We found that the acetylation fluorescence signals of H3/K9 and H4/K12 on chromatin appeared intensively in the germinal vesicle (GV), late-GV (L-GV), and germinal vesicle breakdown (GVBD) stages and became weak in metaphase I (MI); however staining reappeared in anaphase I-telophase-I (AI-TI) and metaphase II (MII). Furthermore, staining was detected in the first polar bodies. The fluorescence signals of H4/K5 first appeared in the MI stage and became intensive in the AI-TI stage; however they were barely detectable in MII stage chromosomes and first polar bodies. We conclude that the acetylation patterns of H3/K9 and H4/K12 during oocyte meiotic maturation are similar and that the pattern of H4/K5 is unique.
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, P. R. China.
authors.forename: Lian-Sheng
authors.initials: LS
authors.lastname: Tang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Xiong
authors.forename: Yi
authors.initials: Y
authors.lastname: Hou
authors.forename: Yong-Zhong
authors.initials: YZ
authors.lastname: Zhang
authors.forename: Qing-Yuan
authors.initials: QY
authors.lastname: Sun
authors.forename: Shu-Ying
authors.initials: SY
authors.lastname: Wang
mesh_terms: D000107:Acetylation; D000123:Acetyltransferases / Q000378:metabolism*; D000818:Animals; D018929:Cell Culture Techniques; D005260:Female; D006657:Histones / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism*; D008540:Meiosis / Q000502:physiology; D009865:Oocytes / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D012756:Sheep
chemical_list: D006657:Histones; D000123:Acetyltransferases; D008239:Lysine