Sensitive detection of protein by an aptamer-based label-free fluorescing molecular switch.
We report an aptamer-based method for the sensitive detection of proteins by a label-free fluorescing molecular switch (ethidium bromide), which shows promising potential in making protein assay simple and economical.
Li
Bingling
B
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, P R China.
Wei
Hui
H
Dong
Shaojun
S
D052157:Aptamers, Nucleotide / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D004996:Ethidium / Q000737:chemistry*; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D011506:Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods; D013917:Thrombin / Q000032:analysis / Q000235:genetics / Q000378:metabolism
D052157:Aptamers, Nucleotide; D016376:Oligonucleotides, Antisense; D011506:Proteins; D013917:Thrombin; D004996:Ethidium