Highly sensitive quantitative analysis of nicotianamine using LC/ESI-TOF-MS with an internal standard.
A highly sensitive quantitative method for analyzing nicotianamine (NA) by liquid chromatography/electrospray ionization time-of-flight mass spectrometry (LC/ESI-TOF-MS) is reported. Fluorenylmethoxycarbonylation of nicotianamine reduced its polarity and enabled its retention in a reversed-phase column. The adoption of N(epsilon)-nicotyllysine (NL) as an internal standard ensured reliable quantification by giving a linear calibration curve drawn between the NA/NL molar ratios of standard solutions injected and the NA/NL area ratios in mass chromatograms. The high sensitivity of this analytical method allowed us to measure the amount of NA. This analytical method has applications to all research concerning NA.
Wada
Yasuaki
Y
Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Yamaguchi
Isomaro
I
Takahashi
Michiko
M
Nakanishi
Hiromi
H
Mori
Satoshi
S
Nishizawa
Naoko K
NK
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000032:analysis*; D001383:Azetidinecarboxylic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D012275:Oryza / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D014026:Nicotiana / Q000737:chemistry
D000806:Angiotensin-Converting Enzyme Inhibitors; D007202:Indicators and Reagents; C082893:nicotianamine; D001383:Azetidinecarboxylic Acid