title: Presenilin 2 is secreted in mouse primary neurons: a release enhanced by apoptosis.
abstract: Presenilin 1 (PS1) and presenilin 2 (PS2) are homologous transmembrane proteins genetically associated with Alzheimer disease. As previously reported by our group for PS1, here we demonstrated that, in mouse primary neurons and microglial cells, PS2 C-terminal fragment (CTF) is released by shedding into the extracellular compartment as a soluble form and that this release is 4.07-fold increased during apoptosis. When compared with PS1, PS2 seems to be more susceptible to apoptosis since its secretion is increased 2.8-fold more than PS1. During apoptosis either proteins were colocalized especially within shedded vesicles. The present study suggest an active role for the presenilins CTF on putative target cells.
authors.affiliation: NeuroBioGen Lab-Memory Clinic, IRCCS Centro S. Giovanni di Dio-FBF, AFaR, Brescia, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Ghidoni
authors.forename: Anna
authors.initials: A
authors.lastname: Paterlini
authors.forename: Luisa
authors.initials: L
authors.lastname: Benussi
authors.forename: Giuliano
authors.initials: G
authors.lastname: Binetti
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D051379:Mice; D009474:Neurons / Q000378:metabolism*; D053766:Presenilin-2 / Q000378:metabolism*; D015854:Up-Regulation / Q000502:physiology*
chemical_list: D053766:Presenilin-2; C095101:Psen2 protein, mouse