Determination of dimethindene enantiomers in pharmaceuticals by capillary electrophoresis with carboxyethyl-beta-cyclodextrin.
Cyclodextrin-mediated capillary zone electrophoresis in a hydrodynamically closed separation system with suppressed electroosmotic flow and UV absorbance photometric detection was developed for a high effective separation and quantitation of dimethindene enantiomers in various pharmaceutical formulations (solution, gel, capsules). The running buffer consisted of epsilon-aminocaproic acid (20 mmol/l) adjusted to pH 4.5 with acetic acid, negatively charged carboxyethyl-beta-cyclodextrin (2.5 mg/ml), serving as chiral selector, and 0.1% (w/v) methylhydroxyethylcellulose, serving as an electroosmotic flow suppressor. The proposed method was successfully validated appraising parameters of sensitivity, linearity, precision, accuracy/recovery and robustness and it is useful for routine use.
Mikus
P
P
Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University, Odbojárov 10, SK-832 32 Bratislava, Slovak Republic. mikus@fpharm.uniba.sk
Kubacák
P
P
Valásková
I
I
Havránek
E
E
D002626:Chemistry, Pharmaceutical; D004115:Dimethindene / Q000032:analysis*; D019075:Electrophoresis, Capillary; D005079:Excipients; D007202:Indicators and Reagents; D012015:Reference Standards; D015203:Reproducibility of Results; D013237:Stereoisomerism; D047392:beta-Cyclodextrins / Q000737:chemistry*
D005079:Excipients; D007202:Indicators and Reagents; D047392:beta-Cyclodextrins; C517669:carboxyethyl-beta-cyclodextrin; D004115:Dimethindene