In vivo recovery and survival of red cells after photodynamic treatment with thiopyrylium and red light using a canine model.
Recently, we have shown that thiopyrylium has robust inactivation capabilities against a broad spectrum of pathogens in the human red blood cell (RBC) suspensions while retaining key RBC in vitro properties. The 24-h recovery and survival of canine red cells were measured upon autologus reinfusion of control and phototreated units. The 24-h recovery of control and phototreated RBCs was 75.7 +/- 6.4% and 87.5 +/- 8.5%, respectively. The time for 50% survival of labelled control RBCs was similar to that of phototreated group (206 +/- 58 h vs. 255 +/- 63 h, respectively). Results suggest that thiopyrylium phototreatment does not negatively affect canine RBC in vivo recovery.
Skripchenko
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Holland Laboratory for the Biomedical Sciences, American Red Cross, 15601 Crabbs Branch Way, Rockville, MD 20855, USA. skripcha@usa.redcross.org
Balch
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Mackin
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Wagner
S J
SJ
D000814:Aniline Compounds / Q000494:pharmacology*; D000818:Animals; D001424:Bacterial Infections / Q000517:prevention & control; D001793:Blood Preservation / Q000379:methods*; D002470:Cell Survival; D003666:Decontamination / Q000379:methods; D004285:Dogs; D004910:Erythrocyte Membrane / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects* / Q000382:microbiology / Q000821:virology; D006461:Hemolysis / Q000187:drug effects*; D023421:Models, Animal; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011714:Pyrans / Q000494:pharmacology*; D014777:Virus Diseases / Q000517:prevention & control
D000814:Aniline Compounds; D017319:Photosensitizing Agents; D011714:Pyrans; C084408:2-methyl-4,6-bis(N,N-dimethylaminophenyl)pyrylium