title: Sonoreactor-based technology for fast high-throughput proteolytic digestion of proteins.
abstract: Fast (120 s) and high-throughput (more than six samples at once) in-gel trypsin digestion of proteins using sonoreactor technology has been achieved. Successful protein identification was done by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, MALDI-TOF-MS. Specific identification of the adenylylsulphate reductase alfa subunit from a complex protein mixture from Desulfovibrio desulfuricans ATCC 27774 was done as a proof of the methodology. The new sample treatment is of easy implementation, saves time and money, and can be adapted to online procedures and robotic platforms.
authors.affiliation: REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Quinta da Torre, 2829-516 Monte de Caparica, Portugal.
authors.forename: R
authors.initials: R
authors.lastname: Rial-Otero
authors.forename: R J
authors.initials: RJ
authors.lastname: Carreira
authors.forename: F M
authors.initials: FM
authors.lastname: Cordeiro
authors.forename: A J
authors.initials: AJ
authors.lastname: Moro
authors.forename: L
authors.initials: L
authors.lastname: Fernandes
authors.forename: I
authors.initials: I
authors.lastname: Moura
authors.forename: J L
authors.initials: JL
authors.lastname: Capelo
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry; D003198:Computer Simulation; D045285:Desulfovibrio desulfuricans / Q000201:enzymology; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000737:chemistry; D010447:Peptide Hydrolases; D011506:Proteins / Q000737:chemistry*; D013010:Sonication; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001426:Bacterial Proteins; D011506:Proteins; D050862:Oxidoreductases Acting on Sulfur Group Donors; C019618:adenylylsulfate reductase; D010447:Peptide Hydrolases