Complementary suppression of T cell activation by peritoneal macrophages and CTLA-4-Ig.
Peritoneal macrophages suppressed T lymphocyte activation by amino acid catabolism. CTLA-4-Ig complemented this form of suppression by blocking the costimulation of T cells. Inhibition of the amino acid catabolizing enzymes or blockade of the IFNgamma signaling essential for macrophage-mediated suppression did not impact CTLA-4-Ig efficacy. Except for phytohemagglutinin, T cell responses to superantigen, mitogen, and anti-CD3 were further reduced when CTLA-4-Ig was combined with peritoneal macrophages. The combination of these complimentary forms of immune regulation potently suppresses adaptive immunity.
Yeh
Kenny
K
Department of Biology, Rider University, Lawrenceville, NJ 08648-3099, USA.
Silberman
Daniel
D
Gonzalez
Diana
D
Riggs
James
J
D000069594:Abatacept; D000818:Animals; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D018796:Immunoconjugates / Q000276:immunology* / Q000378:metabolism; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000276:immunology / Q000378:metabolism; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D017737:Macrophages, Peritoneal / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D016513:Mice, SCID; D052247:Nitric Oxide Synthase Type II / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
D018796:Immunoconjugates; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D000069594:Abatacept; D007371:Interferon-gamma; D052247:Nitric Oxide Synthase Type II