JEG-3 cell culture supernatants cause reduced interferon-gamma and interleukin-17 production in mixed-lymphocyte reactions.
OBJECTIVE
Immunoregulatory effects of choriocarcinoma-derived factors on leukocytes have been documented. The present study was designed to investigate the effect of JEG-3 culture supernatants on interferon-gamma (IFN-gamma), interleukin-17 (IL-17) and IL-1beta production in the mixed lymphocyte reactions (MLRs).


METHODS
A human choriocarcinoma cell line JEG-3 was used to test the effects of its culture supernatants on the proliferation and cytokine production in the MLRs. The cell proliferation was assessed using the BrdU incorporation and the amounts of cytokines were measured using enzyme-linked immunosorbent assays.


RESULTS
The JEG-3 culture supernatants caused significantly reduced IFN-gamma and IL-17 production in the MLRs. However, the supernatants did not influence MLR production of IL-1beta.


CONCLUSIONS
IFN-gamma and IL-17 are mainly produced by activated T cells but IL-1beta is primarily produced by monocytes, thus suggesting that immunoregulatory factors of JEG-3 cells selectively inhibit cytokine production by activated T cells rather than that of the monocytes.
Pongcharoen
Sutatip
S
Department of Medicine, Faculty of Medicine, Naresuan University, Phitsanulok 65000, Thailand. sutatipp@nu.ac.th
Niumsup
Pannika R
PR
Sanguansermsri
Donruedee
D
D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002822:Choriocarcinoma / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D017077:Culture Media, Conditioned / Q000494:pharmacology; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism*; D020381:Interleukin-17 / Q000378:metabolism*; D053583:Interleukin-1beta / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism*; D007959:Lymphocyte Culture Test, Mixed; D011247:Pregnancy; D014594:Uterine Neoplasms / Q000276:immunology / Q000378:metabolism / Q000473:pathology
D017077:Culture Media, Conditioned; D020381:Interleukin-17; D053583:Interleukin-1beta; D007371:Interferon-gamma