Survival change of ventral mesencephalon-derived progenitor cells after grafting into unilateral intact adult rat striatum.
Neural transplantation is one of the most promising treatments for neurodegenerative disorders. Survival rates of embryonic dopamine (DA) neurons following transplantation are low, between 2% and 20% in a number of animal models. To further establish survival changes of the transplanted gestational day 13.5 ventral mesencephalic (VM) cells into left intact adult rat striata so that design strategies of increasing survival of DA neurons, the tyrosine hydroxylase (TH) expression of VM-derived progenitor cells has been examined using immunohistochemistry and Western blot analysis. TH immunostaining revealed that the grafted VM cells developed to mature TH-positive neurons strongly at 3 weeks, peaked at 4 weeks, thereafter, gradually dropped following the degenerative expression of the grafted cells at both 5 and 6 weeks after transplantation. Western blot analysis also showed that the TH proteins were maximally expressed at 4 weeks post-grafting. Our finding suggested that the peak of surviving VM-derived TH positive cells occurred approximately 4 weeks after transplantation.
Park
Moon Seok
MS
Division of Life Science, College of Natural Sciences and Applied Life Science (Brain Korea 21), Gyeongsang National University, Chinju, South Korea.
Lee
Hae Young
HY
Li
Shu Peng
SP
Koh
Phil-Ok
PO
Bahk
Jong Yoon
JY
Chung
Bong Chul
BC
Kim
Myeong Ok
MO
D000818:Animals; D015153:Blotting, Western; D002470:Cell Survival / Q000502:physiology; D003342:Corpus Striatum / Q000378:metabolism / Q000502:physiology*; D005260:Female; D007150:Immunohistochemistry; D008636:Mesencephalon / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000502:physiology*; D013997:Time Factors; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D014446:Tyrosine 3-Monooxygenase