New fluorescent tools for watching nanometer-scale conformational changes of single molecules.
Single-molecule biophysics has been serving biology for more than two decades. Fluorescence microscopy is one of the most commonly used tools to identify molecules of interest and to visualize biological events. Here we describe some of the most commonly used fluorescence imaging tools to measure nanoscale movements and the rotational dynamics of biomolecules.
Toprak
Erdal
E
Center for Biophysics and Computational Biology, University of Illinois, Urbana-Champaign, Urbana, Illinois 61801, USA. toprak@uiuc.edu
Selvin
Paul R
PR
D000818:Animals; D001703:Biophysics / Q000379:methods*; D002627:Chemistry, Physical / Q000379:methods; D003599:Cytoskeleton / Q000378:metabolism; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008856:Microscopy, Fluorescence; D015233:Models, Statistical; D008968:Molecular Conformation; D024701:Myosin Type V / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D016011:Normal Distribution; D017785:Photons; D013997:Time Factors
D024701:Myosin Type V