Association of beta-adrenoceptor single nucleotide polymorphisms with resting heart rate.
The association of beta-adrenoceptor (B-AR, including three subgroups: Beta1, Beta2 and Beta3) single nucleotide polymorphisms (SNP) with resting heart rate (RHR) were analyzed. RHR of 107 healthy subjects in the supine were detected by computerized system for 8 minutes. Genotyping SNPs of S/G49 and R/G389 of Beta1-AR and W/R64 of Beta3-AR in these subjects were by means of PCR-restriction fragment length polymorphism (PCR-RFLP) technique. Genotyping SNPs of R/G16 and Q/E27 of Beta1-adrenoceptor in these subjects were by means of allele specific primer-PCR (ASP-PCR) technique. It was found that there was no significant difference among RHRs corresponding above SNPs of B-AR, but the SNP of R/G389 of Beta1-AR, in which there was significant difference among RHRs (P<0.05). Either in the female individuals or in the male, those with Beta1-AR G/G389 genotype had the highest RHR. The females with R/R389 genotype had the lowest RHR, however, the males with R/G389 genotype had the lowest RHR. The RHR was not only associated with R/G389 polymorphism of Beta1-adrenoceptor gene significantly, but also was associated with gender in Chinese.
Xie
Z X
ZX
Dept. of Biomed. Eng., Chongqing Univ., China.
Niu
Y H
YH
Ma
H X
HX
Yin
Y H
YH
Liu
Z Z
ZZ
Li
Z Y
ZY