title: Highly sensitive quantitative analysis of nicotianamine using LC/ESI-TOF-MS with an internal standard.
abstract: A highly sensitive quantitative method for analyzing nicotianamine (NA) by liquid chromatography/electrospray ionization time-of-flight mass spectrometry (LC/ESI-TOF-MS) is reported. Fluorenylmethoxycarbonylation of nicotianamine reduced its polarity and enabled its retention in a reversed-phase column. The adoption of N(epsilon)-nicotyllysine (NL) as an internal standard ensured reliable quantification by giving a linear calibration curve drawn between the NA/NL molar ratios of standard solutions injected and the NA/NL area ratios in mass chromatograms. The high sensitivity of this analytical method allowed us to measure the amount of NA. This analytical method has applications to all research concerning NA.
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Wada
authors.forename: Isomaro
authors.initials: I
authors.lastname: Yamaguchi
authors.forename: Michiko
authors.initials: M
authors.lastname: Takahashi
authors.forename: Hiromi
authors.initials: H
authors.lastname: Nakanishi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Mori
authors.forename: Naoko K
authors.initials: NK
authors.lastname: Nishizawa
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000032:analysis*; D001383:Azetidinecarboxylic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D012275:Oryza / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D014026:Nicotiana / Q000737:chemistry
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D007202:Indicators and Reagents; C082893:nicotianamine; D001383:Azetidinecarboxylic Acid