title: GP35 ANOAL, an abundant acidic glycoprotein of female Anopheles albimanus saliva.
abstract: Salivary glands of female mosquitoes produce proteins, not completely described yet, that participate in carbohydrate and blood feeding. Here, we report an acidic glycoprotein of 35 kDa (GP35 ANOAL) secreted in the saliva of the malaria vector mosquito Anopheles albimanus. GP35 ANOAL is produced exclusively in the distal lateral lobes of adult female salivary glands, it has a pI of 4.45 and is negatively stained by regular silver stain. An 888 bp cDNA clone encoding a predicted product of 240 amino acids has a signal peptide, potential post-translational modification sites, and a disintegrin signature RGD. The GP35 ANOAL sequence depicts high similarities with the 30 kDa saliva allergen of Aedes aegypti, 30 kDa allergen-like hypothetical proteins, and GE-rich proteins present in several Anopheles species, as well as in Ae. albopictus and Culex pipiens quinquefasciatus. The function of this protein family is still unknown.
authors.affiliation: Centro de Investigación Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, Mexico.
authors.forename: F E
authors.initials: FE
authors.lastname: Cázares-Raga
authors.forename: M
authors.initials: M
authors.lastname: González-Lázaro
authors.forename: C
authors.initials: C
authors.lastname: Montero-Solís
authors.forename: L
authors.initials: L
authors.lastname: González-Cerón
authors.forename: F
authors.initials: F
authors.lastname: Zamudio
authors.forename: J
authors.initials: J
authors.lastname: Martínez-Barnetche
authors.forename: J A
authors.initials: JA
authors.lastname: Torres-Monzón
authors.forename: M
authors.initials: M
authors.lastname: Ovilla-Muñoz
authors.forename: J
authors.initials: J
authors.lastname: Aguilar-Fuentes
authors.forename: M H
authors.initials: MH
authors.lastname: Rodríguez
authors.forename: F
authors.initials: F
authors.lastname: de la Cruz Hernández-Hernández
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D001483:Base Sequence; D005260:Female; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D012469:Salivary Glands / Q000378:metabolism
chemical_list: D006023:Glycoproteins; D019476:Insect Proteins; D012333:RNA, Messenger