title: Fast, ultrasensitive virus detection using a Young interferometer sensor.
abstract: We report the application of an integrated optical Young interferometer sensor for ultrasensitive, real-time, direct detection of viruses. We have validated the sensor by detecting herpes simplex virus type 1 (HSV-1), but the principle is generally applicable. Detection of HSV-1 virus particles was performed by applying the virus sample onto a sensor surface coated with a specific antibody against HSV-1. The performance of the sensor was tested by monitoring virus samples at clinically relevant concentrations. We show that the Young interferometer sensor can specifically and sensitively detect HSV-1 at very low concentrations (850 particles/mL). We have further demonstrated that the sensor can specifically detect HSV-1 suspended in serum. Extrapolation of the results indicates that the sensitivity of the sensor approaches the detection of a single virus particle binding, yielding a sensor of unprecedented sensitivity with wide applications for viral diagnostics.
authors.affiliation: Biophysical Engineering, MESA+ Institute for Nanotechnology and Institute for Biomedical Technology, University of Twente, PO Box 217, 7500 AE Enschede, The Netherlands. A.Ymeti@utwente.nl
authors.forename: Aurel
authors.initials: A
authors.lastname: Ymeti
authors.forename: Jan
authors.initials: J
authors.lastname: Greve
authors.forename: Paul V
authors.initials: PV
authors.lastname: Lambeck
authors.forename: Thijs
authors.initials: T
authors.lastname: Wink
authors.forename: Stephan W F M
authors.initials: SW
authors.lastname: van Hövell
authors.forename: Tom A M
authors.initials: TA
authors.lastname: Beumer
authors.forename: Robert R
authors.initials: RR
authors.lastname: Wijn
authors.forename: Rene G
authors.initials: RG
authors.lastname: Heideman
authors.forename: Vinod
authors.initials: V
authors.lastname: Subramaniam
authors.forename: Johannes S
authors.initials: JS
authors.lastname: Kanger
mesh_terms: D000914:Antibodies, Viral; D001769:Blood / Q000821:virology; D018259:Herpesvirus 1, Human / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D007368:Interferometry / Q000379:methods* / Q000706:statistics & numerical data; D036103:Nanotechnology / Q000379:methods; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods* / Q000706:statistics & numerical data; D014780:Viruses / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000914:Antibodies, Viral