title: Understanding proteomics.
abstract: The purpose of this article is to describe proteomics, to discuss the importance of proteomics, to review different methods for protein measurement, and to illustrate how knowledge of proteomics might improve patient care. Among common laboratory determinations are those involving enzymatic (protein) function. Although the presence or activity of proteins may be seen clinically as incidental, proteins represent the engines through which critical life processes ensue. A selected review of the literature is presented to define and explain proteomics and to review the various techniques to measure proteins. A case-study approach is used to illustrate how proteomics can be utilized for detecting and monitoring disease. The human genome has been completely sequenced and proteomics has emerged as a way to unravel the biochemical and physiological mechanisms of diseases at the functional level. This review includes the recent discoveries regarding proteomics and its importance in the detection and treatment of disease.
authors.affiliation: School of Nursing, University of Kansas Medical Center, Kansas City, KS 66160-7504, USA. jpierce@kumc.edu
authors.forename: Janet D
authors.initials: JD
authors.lastname: Pierce
authors.forename: Mahtab
authors.initials: M
authors.lastname: Fakhari
authors.forename: Kathryn V
authors.initials: KV
authors.lastname: Works
authors.forename: J Thomas
authors.initials: JT
authors.lastname: Pierce
authors.forename: Richard L
authors.initials: RL
authors.lastname: Clancy
mesh_terms: D002874:Chromosome Mapping; D015180:Electrophoresis, Gel, Two-Dimensional; D005786:Gene Expression Regulation / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D036281:Genetic Research; D005826:Genetics, Medical / Q000295:instrumentation / Q000379:methods*; D015894:Genome, Human / Q000235:genetics*; D016045:Human Genome Project; D006801:Humans; D013058:Mass Spectrometry; D018410:Pneumonia, Bacterial / Q000150:complications / Q000175:diagnosis / Q000188:drug therapy; D040081:Protein Array Analysis; D025941:Protein Interaction Mapping; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D012128:Respiratory Distress Syndrome / Q000382:microbiology; D020539:Sequence Analysis, Protein
chemical_list: D005819:Genetic Markers