Simultaneous determination of lithospermic acid B and its three metabolites by liquid chromatography/tandem mass spectrometry.
A rapid and sensitive method based on liquid chromatography/tandem mass spectrometry (LC/MS/MS) was developed for the simultaneous determination of lithospermic acid B and its three main O-methylated metabolites in rat serum with silibinin as the internal standard. The calibration curves for LSB, and the three metabolites were linear over the ranges of 16-4096, and 8-2048 ng/ml, respectively, with coefficients of correlation >0.998. For LSB, the intra-assay coefficient of variance (CV) was less than 9.3% and the inter-assay CV was less than 8.9%. The inter-assay mean accuracy was between 92.8% and 104.7%. For the three metabolites, the intra-assay CV was less than 8.7% and the inter-assay CV was less than 9.9%. The inter-assay mean accuracy was between 92.5% and 107.9%. This quantitation method was successfully applied to a pharmacokinetic study of LSB in rats. Also, a total recovery of 5.2% was found in bile after oral administration.
Li
Xiaochuan
X
State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, China.
Yu
Chen
C
Wang
Li
L
Lu
Youli
Y
Wang
Wenyi
W
Xuan
Lijiang
L
Wang
Yiping
Y
D000042:Absorption; D000284:Administration, Oral; D000818:Animals; D001322:Autoanalysis; D001572:Benzofurans / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics*; D001647:Bile Acids and Salts / Q000032:analysis / Q000737:chemistry; D001682:Biological Availability; D002853:Chromatography, Liquid / Q000379:methods*; D053630:Depsides / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics*; D004355:Drug Stability; D005561:Formates / Q000737:chemistry; D007275:Injections, Intravenous; D008745:Methylation; D015394:Molecular Structure; D008970:Molecular Weight; D051381:Rats; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D000077385:Silybin; D012838:Silymarin / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000737:chemistry
D001572:Benzofurans; D001647:Bile Acids and Salts; D053630:Depsides; D005561:Formates; D012838:Silymarin; D014867:Water; C030544:formic acid; D000077385:Silybin; C076944:salvianolic acid B