title: Chemokine receptor expression on allergen-specific T cells in asthma and allergic bronchopulmonary aspergillosis.
abstract: BACKGROUND
  Allergic bronchopulmonary aspergillosis (ABPA) is a rare variant of severe asthma resulting from hypersensitivity to Aspergillus fumigatus (Asp f) present in the airways.
  
  
  OBJECTIVE
  We analyzed the expression of a panel of six chemokine receptors (CCR3, CCR4, CCR8, CCR5, CXCR3 and CXCR4) on total blood CD4(+) T cells and Asp f-specific-T cells in ABPA patients. We hypothesized that chemokine receptor pattern on T cells differs between ABPA patients, non-ABPA allergic asthmatics sensitized to Dermatophagoides pteronyssinus (Der p) or Phleum pratense (Phl p) and healthy controls.
  
  
  METHODS
  We used the fluorescent dye PKH26, a membrane bound marker, to identify accumulated proliferating (cell-sorted PKH26(low)) CD4(+) T cells in response to allergens (Asp f, Der p, Phl p) or recall antigens (PPD and TT). Chemokine receptor expression was analyzed by flow cytometry on proliferating CD3(+) CD4(+) PKH26(low) cells.
  
  
  RESULTS
  Stimulation of CD4(+) T cells with the relevant allergen resulted in different patterns of chemokine receptor expression in ABPA and non-ABPA allergic asthmatics. Upon Asp f exposure, proliferating CD4(+) T cells from ABPA patients down-regulated the expression of CCR4 and CXCR3 while CCR4 and CXCR3 were up-regulated in allergen-specific T cells from non-ABPA allergic asthmatics. Considering each group of patients, the pattern of chemokine receptors expressed on proliferating allergen-specific CD4(+) T cells was similar to that expressed by recall antigen-specific T cells.
  
  
  CONCLUSIONS
  The down-regulation of CCR4 and CXCR3 after allergen exposure in Asp f-specific T cells seems to be a characteristic feature of ABPA patients and requires further evaluation.
authors.affiliation: Service de Pneumologie et Réanimation Respiratoire, Hôpital Antoine-Béclère, Assistance Publique Hôpitaux de Paris, France.
authors.forename: G
authors.initials: G
authors.lastname: Garcia
authors.forename: M
authors.initials: M
authors.lastname: Humbert
authors.forename: F
authors.initials: F
authors.lastname: Capel
authors.forename: A C
authors.initials: AC
authors.lastname: Rimaniol
authors.forename: P
authors.initials: P
authors.lastname: Escourrou
authors.forename: D
authors.initials: D
authors.lastname: Emilie
authors.forename: V
authors.initials: V
authors.lastname: Godot
mesh_terms: D000368:Aged; D001229:Aspergillosis, Allergic Bronchopulmonary / Q000276:immunology* / Q000382:microbiology; D001232:Aspergillus fumigatus / Q000276:immunology; D001249:Asthma / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D019707:Receptors, Chemokine / Q000096:biosynthesis* / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D019707:Receptors, Chemokine