title: Effect of blood handling on extracellular Hsp72 concentration after high-intensity exercise in humans.
abstract: Heat shock protein 72 (Hsp72) has been detected in the peripheral circulation of humans. Because intracellular Hsp72 binds to aggregated proteins, we hypothesized that postexercise plasma-derived Hsp72 concentrations would be greater than serum-derived Hsp72 because of binding of Hsp72 to aggregated clotting proteins in serum. Postexercise serum, heparin, and ethylenediaminetetraacetic acid (EDTA) samples were collected from 9 recreationally active males and were analyzed for Hsp72 by enzyme-linked immunosorbent assay. In line with our hypothesis, EDTA-treated blood was significantly higher in Hsp72 concentration than all other treatments (P < or = 0.001), whilst heparin plasma (LH) was significantly higher than serum derived on ice (SI) and at room temperature (SR) (P < 0.05; EDTA: 6.46 +/- 0.76, LH: 2.73 +/- 2.26, SI: 0.13 +/- 0.24, SR: 0.20 +/- 0.32 ng/mL). Because previous research has tended to report serum data at the lowest point of the detectable range of the assay, it is recommended that EDTA specimen tubes be used in future investigations.
authors.affiliation: School of Sport, Health, and Exercise Sciences, University of Wales, Bangor, George Building, Holyhead Road, Bangor, Gwynedd LL57 2PZ, UK. m.whitham@bangor.ac.uk
authors.forename: M
authors.initials: M
authors.lastname: Whitham
authors.forename: M B
authors.initials: MB
authors.lastname: Fortes
mesh_terms: D001769:Blood; D001774:Blood Chemical Analysis / Q000379:methods*; D004492:Edetic Acid / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D015444:Exercise; D050884:HSP72 Heat-Shock Proteins / Q000097:blood*; D006339:Heart Rate; D006454:Hemoglobins / Q000378:metabolism*; D006461:Hemolysis; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D008297:Male
chemical_list: D050884:HSP72 Heat-Shock Proteins; D006454:Hemoglobins; D006493:Heparin; D004492:Edetic Acid