title: Induction of programmed cell death in aging Prorocentrum donghaiense cells as was evidenced preliminarily by the identification of associated transcripts.
abstract: Prorocentrum donghaiense caused large-scale red tides off Chinese coast in recent years. Expressed sequence tag (EST) analysis was carried out for this dinoflagellate in order to identify the genes involved in its proliferation and death. A cDNA library was constructed for P. donghaiense at late exponential growth phase, and 308 groups of EST were generated, which include 36 contigs and 272 singletons. Among 22 groups showed homologies with known genes, 2 matched significantly with caspase and proliferating cell nuclear antigen. Caspase and proliferating cell nuclear antigen are 2 key proteins involved in programmed cell death. Their identification evidenced preliminarily the induction of PCD in aging P. donghaiense. The identified included also calmodulin and protein phosphatase, two proteins involved in diverse cell processes including PCD by binding to or modifying others.
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
authors.forename: Xiufang
authors.initials: X
authors.lastname: Zhang
authors.forename: G
authors.initials: G
authors.lastname: Yang
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: W
authors.initials: W
authors.lastname: Yu
authors.forename: K
authors.initials: K
authors.lastname: Pan
authors.forename: Ruixiang
authors.initials: R
authors.lastname: Li
authors.forename: M
authors.initials: M
authors.lastname: Zhu
mesh_terms: D020418:Algal Proteins / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D048028:DNA, Algal / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics; D004141:Dinoflagellida / Q000166:cytology* / Q000235:genetics* / Q000254:growth & development; D020224:Expressed Sequence Tags; D015723:Gene Library; D008969:Molecular Sequence Data; D010839:Phytoplankton / Q000166:cytology* / Q000235:genetics* / Q000254:growth & development; D015800:Protozoan Proteins / Q000235:genetics; D017386:Sequence Homology, Amino Acid
chemical_list: D020418:Algal Proteins; D048028:DNA, Algal; D018076:DNA, Complementary; D016054:DNA, Protozoan; D015800:Protozoan Proteins