Calcium alginate entrapped preparations of Aspergillus oryzae beta galactosidase: its stability and applications in the hydrolysis of lactose.
Insoluble concanavalin A-beta galactosidase complex was obtained by using jack bean extract and this complex was crosslinked with glutaraldehyde, in order to maintain the integrity of complex in the presence of its substrate or products. Concanavalin A-beta galactosidase complex retained 92% of the initial enzyme activity whereas crosslinked complex showed 88% activity. Entrapment of concanavalin A-beta galactosidase complex into calcium alginate beads provided suitability to use this preparation in reactors. Temperature- and pH-optima of the various immobilized beta galactosidase preparations were the same as its soluble counterpart. Entrapped crosslinked concanavalin A-beta galactosidase complex retained more than 50% activity after 1h exposure with 4.0 M urea at room temperature. Moreover, entrapped crosslinked concanavalin A-beta galactosidase complex retained 81 and 62% of the original enzymatic activity in the presence of 5% calcium chloride and 5% galactose, respectively. Entrapped crosslinked concanavalin A-beta galactosidase complex preparation was more superior in the continuous hydrolysis of lactose in a batch process as compared to the other entrapped preparations. This entrapped crosslinked concanavalin A-beta galactosidase complex retained 95% activity after seventh repeated use and 93% of its original activity even after 2 months storage at 4 degrees C.
Haider
Toshiba
T
Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, UP, India.
Husain
Qayyum
Q
D000464:Alginates; D001236:Aspergillus oryzae / Q000201:enzymology*; D002122:Calcium Chloride / Q000494:pharmacology; D032449:Canavalia; D003208:Concanavalin A / Q000737:chemistry; D003432:Cross-Linking Reagents; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D005690:Galactose / Q000494:pharmacology; D020723:Glucuronic Acid; D005976:Glutaral; D006603:Hexuronic Acids; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007785:Lactose / Q000378:metabolism; D046911:Macromolecular Substances / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry; D011489:Protein Denaturation / Q000187:drug effects; D013696:Temperature; D014508:Urea / Q000494:pharmacology; D001616:beta-Galactosidase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
D000464:Alginates; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D006603:Hexuronic Acids; D046911:Macromolecular Substances; D010936:Plant Extracts; D003208:Concanavalin A; D020723:Glucuronic Acid; D014508:Urea; D001616:beta-Galactosidase; D007785:Lactose; D002122:Calcium Chloride; D005976:Glutaral; D005690:Galactose