Detection of DNA with Catalytic Beacons Based on Peroxidase-oxidase Oscillating Reaction.
This paper aims at investigating a new method for the detection of DNA with catalytic beacons based on peroxidase-oxidase (PO) oscillation and analytic pulse perturbation technique. Two DNAzymes were constructed by the binding of specific DNA sequence with hemin or by the hybridization of target DNA with the catalytic beacon. Both DNAzymes possessed peroxidase-like activity and perturb the PO oscillator reaction when they were added into the oscillation system. The period and amplitude of oscillation increased significantly by both DNAzymes, which implied the decrease in the average rate of consumption of oxygen in solution, i.e., the decrease of the average rate of NADH oxidation. The results provide a new sensitive method for DNA detection and molecular recognition.
Zeng
Jinhong
J
Chemistry Department of Huazhong University of Science and Technology, Wuhan Hubei 430074, China (e-mail: Jintao032961@163.com).
Liu
Qiong
Q
Xu
Huibi
H
Wang
Hongmei
H
Wang
Jun
J
Cai
Ruxiu
R