[Evaluation of serum pepsinogen I , II and osteopontin co-detection in gastric cancer screening].
OBJECTIVE
To explore the value of combined assay of serum PG and OPN concentration for gastric cancer screening.


METHODS
Pepsinogen I , II and osteopontin (OPN) concentrations in fasting serum were measured by ELISA in 570 subjects, including 144 gastric cancer, 60 dysplasia, 113 atrophic gastritis, 70 erosion or ulcer, 92 superficial gastritis and 91 healthy control. The cut off point for PG and OPN was determined using receiver operator characteristics curves (ROC).


RESULTS
Using a serum PG I concentration < or =80 ng/ml, I: II ration < or =5.0 and OPN concentration > or =34 ng/ml or > or =30.4 ng/ml (based on ROC) for gastric cancer screening,the specificity, positive and negative predictive values were superior to that obtained by PG concentration only. Using a serumPGI concentration < or =50 ng/ml, I : II ration C 5. 0 and OPN concentration > or =35.2 ng/ml or > or =29. 2 ng/ml (based on ROC), the sensitivity, positive and negative predictive values were superior to that obtained by PG concentration only. Combining PG and OPN for gastric cancer screening, both sensitivity and specificity were more than 70% , while with OPN alone, only good specificity can be achieved.


CONCLUSIONS
Combining different serum PG and OPN concentration for gastric cancer screening is superior to PG or OPN only. This may be used as a new method in gastric cancer mass screening.
Gong
Yue-hua
YH
Cancer Institute of The First Affiliated Hospital, China Medical University, Shenyang 110001, China.
Sun
Li-ping
LP
Yuan
Yuan
Y
D005757:Gastritis, Atrophic / Q000097:blood / Q000175:diagnosis; D006801:Humans; D008403:Mass Screening / Q000379:methods*; D053495:Osteopontin / Q000097:blood*; D020116:Pepsinogen A / Q000097:blood*; D020115:Pepsinogen C / Q000097:blood*; D011230:Precancerous Conditions / Q000097:blood / Q000175:diagnosis; D012372:ROC Curve; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013274:Stomach Neoplasms / Q000097:blood / Q000175:diagnosis*; D013276:Stomach Ulcer / Q000097:blood / Q000175:diagnosis
D053495:Osteopontin; D020115:Pepsinogen C; D020116:Pepsinogen A