title: [Use of ultrasensitive time-resolved fluoroimmunoassay for rapid detection of clenbuterol hydrochloride in urine].
abstract: OBJECTIVE
  In order to provide a rapid and selectivity method for the determination of clenbuterol(CBL), an indirect competitive time-resolved fluoroimmunoassay (TRFIA) was developed.
  
  
  METHODS
  Anti-CBL antibody, was raised by immunization against CBL-BSA in rabbits. CBL-OVA was coated by physical adsorption onto the microtitre plate, CBL or sample with CBL as a competitor. Both them were incubated with limited anti- CBL antibody. and a goat antirabbit IgG-Eu3+ conjugate was used as a tracer.
  
  
  RESULTS
  The sensitivity of CBL-TRFIA was 0.01microg/L, and the recovery rate was 99.7%. RSD of CBL-TRFIA was 3.9% . The sensitivity of CBL-TRFIA provided a linear response from 0.01 - 25microg/L, with ED50 of (1.47+/-0.11) microg/L or ED80 of (0.07+/-0.01)microg/L and ED, of (23.6+/- 0.56) microg/L. The cross reactivity of the CBL-TRFIA with salbutamol, epinephrine hydrochloride and epinephrine bitartrate was negligible, while that with isoprenaline hydrochloride was 0.01% . Both CBL-TRFIA and CBL-ELISA test were applied for the quantitative measurement of CBL in the same urine, and the coefficient of correlation was 0.932.
  
  
  CONCLUSIONS
  The CBL-TRFIA could be applied to detect the CBL in urine and it is useful to screening easily for CBL contamination in meat or foods.
authors.affiliation: School of Biotechnology, Southern Yangtze University, Wuxi 214036, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Shi
authors.forename: Biao
authors.initials: B
authors.lastname: Huang
authors.forename: Lian-fen
authors.initials: LF
authors.lastname: Zhang
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Mi
authors.forename: Gan-rong
authors.initials: GR
authors.lastname: Xu
authors.forename: Jian
authors.initials: J
authors.lastname: Jin
mesh_terms: D000818:Animals; D002976:Clenbuterol / Q000652:urine*; D015200:Fluoroimmunoassay / Q000379:methods*; D008297:Male; D011817:Rabbits; D012680:Sensitivity and Specificity
chemical_list: D002976:Clenbuterol