Bacteria-surface interaction in the presence of proteins and surface attached poly(ethylene glycol) methacrylate chains.
This study analyzes the adhesion behavior of the gram positive bacteria, Staphylococcus aureus (S. aureus), and the gram negative bacteria, Escherichia coli (E. coli), on polypyrrole (PPY) surfaces in the presence of poly(ethylene glycol) methacrylate (PEGMA) chains and plasma proteins (bovine serum albumin and bovine plasma fibrinogen) either preadsorbed on the film surface or in the bacterial suspension. Bacterial adhesion experiments performed in a suspension of bacterial cells and protein may give important insights on the behavior of bacterial adhesion in an in vivo environment. Protein adsorption and bacterial adhesion on PEGMA-grafted PPY films were reduced by about a factor of 2-4 compared with those on the pristine PPY films. In addition, the number of bacterial cells adhering on the substrate is dependent not only on the type of protein present, but also the sequence of exposure to the protein relative to the bacteria. Furthermore, bacteria-surface adhesion force was measured using the atomic force microscopy with increasing lateral force to detach the individual cell. The adhesion force of S. aureus is influenced by PEGMA and plasma protein modification and is significantly higher than that of E. coli for all substrates tested. The number of adherent cells on the substrate is shown to be directly correlated to the bacterial adhesion force.
Tedjo
Chrysanty
C
Department of Chemical and Biomolecular Engineering, National University of Singapore, Kent Ridge, Singapore 119260.
Neoh
K G
KG
Kang
E T
ET
Fang
Ning
N
Chan
Vincent
V
D000327:Adsorption; D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D018441:Biofilms / Q000254:growth & development; D002417:Cattle; D020099:Coated Materials, Biocompatible; D004926:Escherichia coli / Q000472:pathogenicity / Q000502:physiology; D005340:Fibrinogen; D066298:In Vitro Techniques; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D011092:Polyethylene Glycols; D011108:Polymers; D016459:Prosthesis-Related Infections / Q000517:prevention & control; D011758:Pyrroles; D012710:Serum Albumin, Bovine; D013057:Spectrum Analysis; D013211:Staphylococcus aureus / Q000472:pathogenicity / Q000502:physiology; D014965:X-Rays
D020099:Coated Materials, Biocompatible; D011108:Polymers; D011758:Pyrroles; C450003:poly(ethylene glycol) methacrylate polymer; D012710:Serum Albumin, Bovine; C067635:polypyrrole; D011092:Polyethylene Glycols; D005340:Fibrinogen