Development of vaccines and passive immunotherapy against SARS corona virus using SCID-PBL/hu mouse models.
We have investigated novel vaccine strategies against severe acute respiratory syndrome (SARS) CoV using cDNA constructs encoding the structural antigens: (S), (M), (E), or (N) protein, derived from SARS CoV. PBL from healthy human volunteers were administered i.p. into IL-2 receptor gamma-chain disrupted SCID mice, and SCID-PBL/hu mice were constructed. These mice can be used to analyze the human immune response in vivo. SARS M DNA vaccine and N DNA vaccine induced human CTL specific for SARS CoV antigens. Alternatively, SARS M DNA vaccines inducing human neutralizing antibodies and human monoclonal antibodies against SARS CoV are now being developed. These results show that these vaccines can induce virus-specific immune responses and should provide a useful tool for development of protective and therapeutic vaccines.
Okada
Masaji
M
Clinical Research Center, National Hospital Organization Kinki-Chuo Chest Medical Center, 1180 Nagasone, Kitaku, Sakai, Osaka 591-8555, Japan. okm@kch.hosp.go.jp
Okuno
Yoshinobu
Y
Hashimoto
Satomi
S
Kita
Yoko
Y
Kanamaru
Noriko
N
Nishida
Yasuko
Y
Tsunai
Yoshie
Y
Inoue
Ruriko
R
Nakatani
Hitoshi
H
Fukamizu
Reiko
R
Namie
Yumi
Y
Yamada
Junko
J
Takao
Kyoko
K
Asai
Ritsuko
R
Asaki
Ryoko
R
Kase
Tetsuo
T
Takemoto
Yuji
Y
Yoshida
Shigeto
S
Peiris
J S M
JS
Chen
Pei-Jer
PJ
Yamamoto
Naoki
N
Nomura
Tatsuji
T
Ishida
Isao
I
Morikawa
Shigeru
S
Tashiro
Masato
M
Sakatani
Mitsunori
M
D000818:Animals; D004195:Disease Models, Animal; D007116:Immunization, Passive / Q000379:methods*; D007167:Immunotherapy; D051379:Mice; D016513:Mice, SCID; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D045169:Severe Acute Respiratory Syndrome / Q000517:prevention & control*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics; D014765:Viral Vaccines / Q000008:administration & dosage*
D019444:Vaccines, DNA; D014765:Viral Vaccines