title: CNTF promotes the survival and differentiation of adult spinal cord-derived oligodendrocyte precursor cells in vitro but fails to promote remyelination in vivo.
abstract: Delivery of factors capable of promoting oligodendrocyte precursor cell (OPC) survival and differentiation in vivo is an important therapeutic strategy for a variety of pathologies in which demyelination is a component, including multiple sclerosis and spinal cord injury. Ciliary neurotrophic factor (CNTF) is a neuropoietic cytokine that promotes both survival and maturation of a variety of neuronal and glial cell populations, including oligodendrocytes. Present results suggest that, although CNTF has a potent survival and differentiation promoting effect in vitro on OPCs isolated from the adult spinal cord, CNTF administration in vivo is not sufficient to promote oligodendrocyte remyelination in the glial-depleted environment of unilateral ethidium bromide (EB) lesions.
authors.affiliation: MD/PhD Program, University of Louisville, Louisville, KY 40292, USA.
authors.forename: Jason F
authors.initials: JF
authors.lastname: Talbott
authors.forename: Qilin
authors.initials: Q
authors.lastname: Cao
authors.forename: James
authors.initials: J
authors.lastname: Bertram
authors.forename: Michael
authors.initials: M
authors.lastname: Nkansah
authors.forename: Richard L
authors.initials: RL
authors.lastname: Benton
authors.forename: Erin
authors.initials: E
authors.lastname: Lavik
authors.forename: Scott R
authors.initials: SR
authors.lastname: Whittemore
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D020934:Ciliary Neurotrophic Factor / Q000494:pharmacology*; D004996:Ethidium; D009186:Myelin Sheath / Q000187:drug effects; D009416:Nerve Regeneration / Q000187:drug effects; D009836:Oligodendroglia / Q000166:cytology* / Q000502:physiology*; D051381:Rats; D013116:Spinal Cord / Q000166:cytology* / Q000503:physiopathology; D013118:Spinal Cord Diseases / Q000139:chemically induced / Q000503:physiopathology; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology*
chemical_list: D020934:Ciliary Neurotrophic Factor; D004996:Ethidium