[Effect of dexamethasone on expression of intercellular adhesion molecule-1 in orbital fibroblasts from thyroid associated ophthalmopathy].
OBJECTIVE
To investigate the effects of dexamethasone (DEX) on the expression of intercellular adhesion molecule-1 (ICAM-1) in orbital fibroblasts (OF) from patients with thyroid associated ophthalmopathy (TAO).


METHODS
OFs from 10 patients with TAO and from 5 normal control subjects were cultured in vitro. OFs were incubated with DEX, IFN-gamma or DEX and IFN-gamma compound for 72 hours. Flow cytometry analyses were performed to determine ICAM-1 expression.


RESULTS
Baral ICAM-1 expression in OFs from TAO was significantly higher than that in the controls. DEX (10(-6) mol/L) inhibited expression of ICAM-1. IFN-gamma (300 U/ mL) stimulated ICAM-1 expression significantly, which was inhibited by DEX (10(-6) mol/L).


CONCLUSIONS
ICAM-1 plays an important role in the pathogenesis of TAO. DEX not only inhibits ICAM-1 expression, but also inhibits IFN-gamma-induced ICAM-1 expression on cultured OF in vitro. This may be part of the mechanism by which glucocorticoid has efficacy in the treatment of TAO.
He
Wei-min
WM
Department of Ophthalmology, West China Hospital, Shichuan University, Chengdu 610041, China.
Luo
Qing-li
QL
Zeng
Ji-hong
JH
D000328:Adult; D016022:Case-Control Studies; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology* / Q000627:therapeutic use; D005260:Female; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects*; D049970:Graves Ophthalmopathy / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008297:Male; D009915:Orbit / Q000473:pathology*
D018799:Intercellular Adhesion Molecule-1; D003907:Dexamethasone