HHV-6 DNA in peripheral blood mononuclear cells after liver transplantation.
BACKGROUND
Human herpesvirus-6 (HHV-6) infections have been reported after liver transplantation. In this study, the detection of HHV-6 DNA in peripheral blood mononuclear cells (PBMC) was compared with HHV-6 antigenemia in liver transplant patients.


OBJECTIVE
Forty-three adult liver recipients were frequently monitored by HHV-6 antigenemia test, which detects the viral antigens in PBMC, but is rather qualitative than quantitative.


METHODS
From the same PBMC specimens HHV-6 DNA was demonstrated by in situ hybridization using a biotinylated probe and quantified as positive cells/10, 000 PBMC. Altogether 330 blood specimens were analyzed.


RESULTS
During the first 6 months (mean 12 days) after transplantation, 35/43 patients developed HHV-6 antigenemia. Concurrently, HHV-6 DNA-positive cells with mean peak number of 661(+/-574)/10, 000 were detected in 33/35 patients. Seven patients received ganciclovir treatment because of concurrent CMV infection with mean peak number of HHV-6 DNA-positive cells 381(+/-336) before and 34(+/-59)/10, 000 after the treatment (p = 0.03). All CMV infections responded to ganciclovir, but HHV-6 DNAemia disappeared slowly, within 79 days (mean 36 days). Without antivirals, HHV-6 DNAemia/antigenemia lasted usually longer.


CONCLUSIONS
Detection of HHV-6 DNA in PBMC correlated well with HHV-6 antigenemia, and may be used in the monitoring of transplant patients.
Loginov
Raisa
R
Department of Virology, HUSLAB, Helsinki University Central Hospital and University of Helsinki, FIN-00290 Helsinki, Finland.
Härmä
Maiju
M
Halme
Leena
L
Höckerstedt
Krister
K
Lautenschlager
Irmeli
I
D000956:Antigens, Viral / Q000032:analysis; D003586:Cytomegalovirus Infections / Q000188:drug therapy; D004279:DNA, Viral / Q000032:analysis; D015774:Ganciclovir / Q000627:therapeutic use; D015654:Herpesvirus 6, Human / Q000302:isolation & purification*; D006801:Humans; D017403:In Situ Hybridization; D007963:Leukocytes, Mononuclear / Q000821:virology*; D017093:Liver Failure / Q000150:complications* / Q000601:surgery; D016031:Liver Transplantation; D019349:Roseolovirus Infections / Q000821:virology*
D000956:Antigens, Viral; D004279:DNA, Viral; D015774:Ganciclovir