title: Application of pressure cycling technology to tissue sample preparation for 2-DE.
abstract: 2-DE is a powerful protein analytical tool whose major strengths include semiglobal quantitation and charge separation of complex protein mixtures, enabling the analysis of differential protein expression, and variable post-translational modification. One of 2-DE's limitations relates to its limited dynamic range and consequently the number of proteins expressed that can be analyzed on a single gel. In an attempt to improve the yield of detectable proteins during sample preparation, we applied a novel extraction technique called pressure cycling technology.
authors.affiliation: Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, IN 46202-2111, USA.
authors.forename: Heather
authors.initials: H
authors.lastname: Ringham
authors.forename: Richard L
authors.initials: RL
authors.lastname: Bell
authors.forename: Gary B
authors.initials: GB
authors.lastname: Smejkal
authors.forename: James
authors.initials: J
authors.lastname: Behnke
authors.forename: Frank A
authors.initials: FA
authors.lastname: Witzmann
mesh_terms: D053000:Analytic Sample Preparation Methods; D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D006874:Hydrostatic Pressure; D011506:Proteins / Q000032:analysis*; D051381:Rats
chemical_list: D011506:Proteins