[The expression of gene encoding folitropin receptor in granulosa cells in women smoking cigarettes and nonsmokers participating in vitro fertilization programme].
This research was stated on the group of infertile patient taking part in in vitro fertilization. The whole group of 60 random patients was divided into two subgroups: 10 persons smoking cigarettes and 50 nonsmokers. The number of follicles obtained after controlled ovarian hiperstimulation, the number of mature and immature oocytes and concentration of 17beta-estradiol in serum at the hCG day were measured for all women. There was also calculated the number of granulosa cells and the relative expression of the gene encoding FSHR. Comparing all testing groups, in smokers the worse ovarian answer for hiper-stimulation was observed. It was characterized by both the lower number of follicles and oocytes and also the lower concentration of estradiol in serum at hCG day. There was also observed lower number of granulosa cells in fluid from all folicles at diameter > 12 mm in smoking patients compared with nonsmokers. Analizing the relative expression of gene coding FSHR in granulosa cells significant differences between these two groups were demonstrated. The evaluation of the expression level showed its reduction in smokers (2). To compare it the average level of expression in nonsmokers was 24. Smoking cigarettes is correlated with the lower number of granulosa cells and the lower expression level of the gene coding for folitropin receptor.
Jósiak
Magdalena
M
Klinika Niepłodności i Endokrynologii Rozrodu, Katedry Ginekologii i Połoznictwa, Akademii Medycznej w Poznaniu.
Jedrzejczak
Piotr
P
Depa-Martynów
Magdalena
M
Pawelczyk
Leszek
L
D000328:Adult; D015536:Down-Regulation; D004958:Estradiol / Q000097:blood; D005260:Female; D005307:Fertilization in Vitro; D005786:Gene Expression Regulation; D006107:Granulosa Cells / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007247:Infertility, Female / Q000503:physiopathology* / Q000628:therapy*; D009865:Oocytes / Q000166:cytology / Q000187:drug effects; D011247:Pregnancy; D011962:Receptors, FSH / Q000235:genetics*; D012907:Smoking / Q000503:physiopathology*; D016896:Treatment Outcome
D011962:Receptors, FSH; D004958:Estradiol