Novel Shear-horizontal Surface Acoustic Wave Based Immunosensors Using SiO<inf>2</inf>Waveguiding Layers And Flow Injection Analysis.
Surface acoustic wave (SAW) devices based on shear-horizontal (SH) waves can be used as mass-sensitive immunosensors. This paper presents a novel SH-SAW sensor to detect anti-immunoglobulin (IgG) molecules by means of the antibody-antigen binding mechanism. The sensor system comprising dual delay lines was fabricated on 36&#176; Y-X LiTaO<inf>3</inf>substrate. A SiO<inf>2</inf>layer was used as love mode waveguiding layers, well as insulating and chemically resistant protective layer. Moreover, flow injection analysis (FIA) method was used for continuous detection the protein molecules. The protein A was immobilized on the optional surface of the gold layer, then coupled with IgG to adsorb the antigens to be measured in the protein solution. The operational frequency of the system changed due to the interaction of antibody-antigen binding. The experimental result demonstrates the sensor has stable frequency response to the mass loading effect of the various anti-IgG concentrations with the sensitivity up to 3.3ng/ml/Hz.
Guo
X S
XS
Biosensor National Special Laboratory, Dept. of Biomedical Engineering, Zhejiang University, Hangzhou 310027, P.R.China; College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310027, P.R.China. E-mail: guoxszju@yahoo.com.cn.
Chen
Y Q
YQ
Yang
X L
XL
Wang
L R
LR