Preventive effects of Ginkgo biloba extract on ischemia-reperfusion injury in rat bladder.
OBJECTIVE
The aim of this experimental study was to evaluate the effects of Ginkgo biloba (EGb 761) on ischemia-reperfusion (I-R) injury in the rat bladder.


METHODS
A bladder I-R injury was induced by abdominal aorta occlusion by ischemia for 30 min, followed by 45 min reperfusion in rats. The rats were divided into four groups of 7 rats each; the control, I-R, and I-R groups were pretreated intraperitoneally with 50 or 100 mg/kg G. biloba 60 min before ischemia induction. Contractile responses to carbachol through isolated organ bath studies were recorded, histological sections were evaluated by light microscopy, and TUNEL staining was performed for the evaluation of apoptosis.


RESULTS
In the I-R group, the contractile responses of the bladder strips were lower than those of the control group (p < 0.01-0.001) and were restored by pretreatment with 100 mg/kg G. biloba (p < 0.05-0.001). Decreased polymorphonuclear leukocyte infiltration was detected in the G. biloba pretreatment groups when compared to the I-R group during histological evaluation. The ratio of TUNEL-positive nuclei was 1.84% in the I-R group, whereas it was decreased in both of the G. biloba pretreatment groups (p < 0.01, p < 0.01).


CONCLUSIONS
Our data indicated that G. biloba has a preventive effect on I-R injury in rat urinary bladder.
Yenilmez
Aydin
A
Department of Urology, Eskisehir Osmangazi University Medical Faculty, Eskisehir, Turkey. aydinyenilmez@yahoo.com
Kilic
Fatma Sultan
FS
Sirmagul
Basar
B
Isikli
Burhanettin
B
Aral
Erinc
E
Oner
Setenay
S
D000818:Animals; D002317:Cardiovascular Agents / Q000627:therapeutic use*; D020441:Ginkgo biloba; D008297:Male; D009119:Muscle Contraction; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*; D001743:Urinary Bladder / Q000098:blood supply*; D000096983:Ginkgo Extract
D002317:Cardiovascular Agents; D010936:Plant Extracts; C063170:Ginkgo biloba extract; D000096983:Ginkgo Extract