Preparation of protein-silicate hybrids from polyamine intercalation of layered montmorillonite.
Hybrids of the model BSA protein and layered silicate clay with d spacing of approximately 62 A were prepared from either direct or stepwise intercalation. The pristine montmorilloinite (Na+-MMT) was first modified by poly(oxyalkylene)-amine salts (POP- and POE-amine) of 2000 g/mol Mw to a gallery-expanded silicate (d spacing=53 and 18 A, respectively), which became accessible for BSA protein embedding. Subsequent BSA substitution allowed the embedding of the protein into the layered clay galleries in an uncompressed conformation. The stepwise process of embedding large molecules into the silicate gallery provides a new method for synthesizing biomaterial/clay hybrids potentially useful in drug delivery or biomedical design.
Lin
Jiang-Jen
JJ
Institute of Polymer Science and Engineering, National Taiwan University, Taipei 10617, Taiwan. jianglin@ntu.edu.tw
Wei
Jiun-Chiou
JC
Juang
Tzong-Yuan
TY
Tsai
Wei-Cheng
WC
D000327:Adsorption; D000588:Amines / Q000737:chemistry; D000818:Animals; D001546:Bentonite / Q000737:chemistry*; D002417:Cattle; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D011073:Polyamines / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D017640:Silicates / Q000737:chemistry*; D013696:Temperature; D014961:X-Ray Diffraction
D000588:Amines; D011073:Polyamines; D017640:Silicates; D001546:Bentonite; D012710:Serum Albumin, Bovine