Cloning of a rat-specific long PCP4/PEP19 isoform.
We report the identification of a cDNA that encodes a putative protein of 94 amino acids and expected molecular weight of 10.7 kDa, the C-terminal half of which is identical to that of PEP19, a small, brain-specific protein involved in Ca++/calmodulin signaling. The novel rat-specific protein, tentatively named long PEP19 isoform (LPI), is the product of alternative splicing of the rat PCP4 gene encoding PEP19. We found that antibodies raised against the first 13 N-terminal amino acids of LPI, not present in PEP19, recognize a protein enriched in the developing rat brain.
Sala
Anna
A
Dipartimento di Scienze Biochimiche, University of Palermo, Palermo, Italy.
Scaturro
Maria
M
Proia
Patrizia
P
Schiera
Gabriella
G
Balistreri
Epifania
E
Aflalo-Rattenbach
Revital
R
Créau
Nicole
N
Di Liegro
Italia
I
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D002148:Calmodulin-Binding Proteins; D002457:Cell Extracts; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D005091:Exons / Q000235:genetics; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D007438:Introns / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D010455:Peptides / Q000276:immunology; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D000906:Antibodies; D002148:Calmodulin-Binding Proteins; D002457:Cell Extracts; D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; C489090:Pcp4 protein, rat; D010455:Peptides; D020033:Protein Isoforms; D012333:RNA, Messenger; D016601:RNA-Binding Proteins