Comparative evaluation of western blotting in hepatic and pulmonary cystic echinococcosis.
Many serological tests are widely used in the diagnosis of cystic echinococcosis (CE), caused by the larval stages of Echinococcus granulosus. The present study was carried for differentiation between hepatic and pulmonary cystic echinococcosis by Western Blotting (WB). A total of 121 sera from patients with hepatic CE (37), pulmonary CE (31) and controls (53; consisting of six healthy, seven Hymenolepis nana infection, 20 hepatic and 20 pulmonary diseases other than CE) were examined. In all of the CE patients, E. gronulosus infection was confirmed by surgical intervention. Sera were previously tested using IHA and ELISA to detect the E. gronulosus specific antibodies. Sera from hepatic cases of CE reacted with 16 polypeptides of 6-116 kDa and sera from pulmonary cases of CE reacted with 14 polypeptides of 4-130 kDa by Western Blotting. The WB test enabled the detection of antibodies in the hepatic CE samples for proteins of 24, 32 34, 44-46 and 52-54 kDa in molecular weight in 78.4%, 75.7%, 78.4% and 89.2% of the patients, respectively. In the pulmonary CE samples sera WB test enabled the detection of antibodies 24, 44-46, 100, 110, 116 and 120 124 kDa in molecular weight in 81.3%, 75.0%, 87.5%, 71.9%, 84.4% and 65.6% of the patients, respectively. We indicated that the antigenic components of high molecular weight can be good candidates for differentiation of hepatic CE from pulmonary CE.
Akisu
C
C
Dokuz Eylul University, Medical Faculty, Department of Parasitology, Izmir, Turkey. ciler.akisu@deu.edu.tr
Delibas
S B
SB
Bicmen
C
C
Ozkoc
S
S
Aksoy
U
U
Turgay
N
N
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D015153:Blotting, Western / Q000379:methods*; D003551:Cysticercosis / Q000175:diagnosis* / Q000601:surgery; D003937:Diagnosis, Differential; D004444:Echinococcosis, Hepatic / Q000175:diagnosis* / Q000601:surgery; D004445:Echinococcosis, Pulmonary / Q000175:diagnosis* / Q000601:surgery; D048209:Echinococcus granulosus / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight
D000909:Antibodies, Helminth