title: Effects of mitogenic hormones on HSP70 expression in a silver sea bream fibroblast cell line and a primary macrophage preparation.
abstract: This study was performed in order to elucidate the role and importance of three mitogenic hormones [growth hormone (GH), insulin-like growth factor-I (IGF-1) and prolactin (PRL)] on heat shock protein 70 (HSP70) expression in a silver sea bream fibroblast cell line and a primary macrophage preparation. Fibroblasts and macrophages that were exposed to GH at concentrations of 1-1000ng/ml did not exhibit modulated HSP70 expression, whereas GH at an exposure concentration of 10ng/ml lowered HSP70 levels in macrophages. Upon exposure to IGF-1 it was found that HSP70 expression remained unchanged in fibroblasts but was significantly decreased in macrophages at exposure concentrations of 1-10ng/ml. Finally, and using a preparation of ovine PRL it was found that HSP70 expression decreased in fibroblasts at exposure concentrations of 1-1000ng/ml and also decreased in macrophages at exposure concentrations of 1-100ng/ml.
authors.affiliation: Department of Biology, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR, China.
authors.forename: Eddie E
authors.initials: EE
authors.lastname: Deane
authors.forename: Liran
authors.initials: L
authors.lastname: Zhou
authors.forename: Norman Y S
authors.initials: NY
authors.lastname: Woo
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D013006:Growth Hormone / Q000494:pharmacology; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006728:Hormones / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008934:Mitogens / Q000494:pharmacology; D010587:Phagocytosis / Q000187:drug effects / Q000502:physiology; D011388:Prolactin / Q000494:pharmacology; D021541:Sea Bream
chemical_list: D018840:HSP70 Heat-Shock Proteins; D006728:Hormones; D008934:Mitogens; D007334:Insulin-Like Growth Factor I; D011388:Prolactin; D013006:Growth Hormone