title: Neutrophils invade lumbar dorsal root ganglia after chronic constriction injury of the sciatic nerve.
abstract: To test whether neutrophils (PMN) target lumbar dorsal root ganglia (DRG) following axonal injury leading to neuropathic pain, we visualized PMN infiltration in DRG tissue sections and estimated PMN count by flow cytometry following sciatic chronic constriction injury (CCI). Seven days after CCI, results show PMN within DRG where their count increased by three fold ipsilateral to injury compared to contralateral or sham, concomitant with peak neuropathic pain behavior. Superoxide burst in PMN isolated from rats d7 after CCI was elevated by 170% +/-18 compared to naïve and MCP-1 mRNA expression in DRG increased by 8.9+/-2.9 fold, but that of MIP-2, CINC-1, and RANTES did not change. We conclude that CCI causes PMN invasion of the DRG whereby the functional implication of their close proximity to neuronal axon and soma remains unknown.
authors.affiliation: Department of Surgery, Rhode Island Hospital, Brown Medical School, Providence, RI 02903, USA.
authors.forename: N
authors.initials: N
authors.lastname: Morin
authors.forename: S A
authors.initials: SA
authors.lastname: Owolabi
authors.forename: M W
authors.initials: MW
authors.lastname: Harty
authors.forename: E F
authors.initials: EF
authors.lastname: Papa
authors.forename: T F
authors.initials: TF
authors.lastname: Tracy
authors.forename: S K
authors.initials: SK
authors.lastname: Shaw
authors.forename: M
authors.initials: M
authors.lastname: Kim
authors.forename: C Y
authors.initials: CY
authors.lastname: Saab
mesh_terms: D000818:Animals; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism; D003250:Constriction; D007839:Functional Laterality; D005727:Ganglia, Spinal / Q000473:pathology*; D005786:Gene Expression Regulation / Q000502:physiology; D008161:Lumbosacral Region; D008297:Male; D009504:Neutrophils / Q000502:physiology*; D010147:Pain Measurement / Q000379:methods; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020426:Sciatic Neuropathy / Q000473:pathology* / Q000503:physiopathology*; D013997:Time Factors
chemical_list: C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D012333:RNA, Messenger