Role of DNA repair by nonhomologous-end joining in Bacillus subtilis spore resistance to extreme dryness, mono- and polychromatic UV, and ionizing radiation.
The role of DNA repair by nonhomologous-end joining (NHEJ) in spore resistance to UV, ionizing radiation, and ultrahigh vacuum was studied in wild-type and DNA repair mutants (recA, splB, ykoU, ykoV, and ykoU ykoV mutants) of Bacillus subtilis. NHEJ-defective spores with mutations in ykoU, ykoV, and ykoU ykoV were significantly more sensitive to UV, ionizing radiation, and ultrahigh vacuum than wild-type spores, indicating that NHEJ provides an important pathway during spore germination for repair of DNA double-strand breaks.
Moeller
Ralf
R
Space Life Sciences Laboratory, Building M6-1025/SLSL, Kennedy Space Center, FL 32953, USA.
Stackebrandt
Erko
E
Reitz
Günther
G
Berger
Thomas
T
Rettberg
Petra
P
Doherty
Aidan J
AJ
Horneck
Gerda
G
Nicholson
Wayne L
WL
D001412:Bacillus subtilis / Q000502:physiology* / Q000528:radiation effects*; D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair; D011839:Radiation, Ionizing; D013171:Spores, Bacterial / Q000502:physiology / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects; D014618:Vacuum