[Effects of carbon source and nitrogen source on callus growth and flavonoid content in Glycyrrhiza uralensis].
OBJECTIVE
To study the effects of carbon source and nitrogen source on callus growth, and flavonoid content in Glycyrrhiza uralensis.


METHODS
Induction and culture of callus were conducted in the media of different concentrations of sucrose, and fructose and ratio of NH4+/NO3- ,and flavonoid content was measured by HPLC.


RESULTS
The results showed that fructose was superior to sucrose for callus growth and flavonoid formation, and the optimum concentration was 2%. The flavonoids content was 2 times higher than that of sucrose as carbon sources. In the range of 2% -6% of fructose concentration, the flavonoid content was decreased along with the concentration of fructose, but the licochalcone was increased 5-fold. The highest flavonoid content of 151.47 microg x g(-1) was obtained when the ratio of NH4+/NO3- in the medium was 1/2. NH; inhibited the callus growth and flavonoid formation of G. uralensis.


CONCLUSIONS
Fructose as carbon source was superior to sucrose for callus growth and flavonoid formation, and NO3- was favorable to the callus growth and flavonoids accumulation.
Yang
Shi-hai
SH
College of Chinese Medicinal Material, Jilin Agricultural University, Changchun 130118, China. jlyangs@yahoo.com.cn
Tao
Jing
J
Liu
Xiao-feng
XF
Guo
De-an
DA
Zheng
Jun-hua
JH
D003470:Culture Media / Q000494:pharmacology; D004187:Disaccharides / Q000494:pharmacology*; D005419:Flavonoids / Q000096:biosynthesis*; D005632:Fructose / Q000494:pharmacology; D029882:Glycyrrhiza uralensis / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D009566:Nitrates / Q000494:pharmacology; D017672:Nitrogen Compounds / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology; D046509:Tissue Culture Techniques
D003470:Culture Media; D004187:Disaccharides; D005419:Flavonoids; D009566:Nitrates; D017672:Nitrogen Compounds; D000644:Quaternary Ammonium Compounds; D005632:Fructose; D013395:Sucrose