Biomonitoring in a clean and a multi-contaminated estuary based on biomarkers and chemical analyses in the endobenthic worm Nereis diversicolor.
Relationships between biochemical and physiological biomarkers (acetylcholinesterase [AChE], catalase, and glutathione S-transferase [GST] activities, thiobarbituric acid reactive substances, glycogen, lipids and proteins) and accumulated concentrations of contaminants (polychlorinated biphenyls [PCBs], polycyclic aromatic hydrocarbons and metals) were examined in the keystone species Nereis diversicolor. The chemical analyses of worms and sediments allowed the designation of the Seine estuary and the Authie estuary as a polluted and relatively clean site respectively. Worms from the Seine estuary exhibited higher GST and lower AChE activities. Generally, larger worms had higher concentrations of energy reserves. Principal component analyses clearly highlighted intersite differences: in the first plan, GST activities and chemical concentrations were inversely related to concentrations of energy reserves; in the second one, PCB concentrations and AChE activity were inversely related. Depleted levels of energy reserves could be a consequence of combating toxicants and might predict effects at higher levels of biological organization. The use of GST and AChE activities and energy reserve concentrations as biomarkers is validated in the field in this keystone species.
Durou
Cyril
C
CNRS, Université de Nantes, Pôle Mer et Littoral, SMAB, 2 rue de la Houssinière, BP 92208, F-44322 Nantes Cedex 3, France. cyril.durou@uco.fr
Poirier
Laurence
L
Amiard
Jean-Claude
JC
Budzinski
Hélène
H
Gnassia-Barelli
Mauricette
M
Lemenach
Karyn
K
Peluhet
Laurent
L
Mouneyrac
Catherine
C
Roméo
Michèle
M
Amiard-Triquet
Claude
C
D000110:Acetylcholinesterase / Q000032:analysis; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002374:Catalase / Q000032:analysis; D004734:Energy Metabolism; D004784:Environmental Monitoring / Q000379:methods; D005602:France; D005982:Glutathione Transferase / Q000032:analysis; D006003:Glycogen / Q000378:metabolism; D050356:Lipid Metabolism; D011077:Polychaeta / Q000737:chemistry* / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000032:analysis; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis; D025341:Principal Component Analysis / Q000379:methods; D011506:Proteins / Q000378:metabolism; D045483:Rivers / Q000737:chemistry; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis*
D015415:Biomarkers; D011084:Polycyclic Aromatic Hydrocarbons; D011506:Proteins; D017392:Thiobarbituric Acid Reactive Substances; D014874:Water Pollutants, Chemical; D006003:Glycogen; D011078:Polychlorinated Biphenyls; D002374:Catalase; D005982:Glutathione Transferase; D000110:Acetylcholinesterase