[DNA extraction from formalin fixed and paraffin embedded tissues].
Owing to the DNA degradation induced by formalin and the obstruction of paraffin to DNA extraction, it is difficult to recover high-quality DNA from Formalin fixed and paraffin embedded tissue (FFPET). In recent years, a lot of researches indicate that the DNA extraction from FFPET can be developed by improving the pretreatment, optimizing the digestion condition of proteinase, simplizing the procedures of the DNA extraction, purifying the extracted DNA and so on, which may pave a way for popularizing FFPET in DNA analysis.
Tan
Zhuo-yi
ZY
School of Forensic Medicine, China Medical University, Shenyang 110001, China. kukler@sina.com
Ding
Mei
M
D004247:DNA / Q000302:isolation & purification*; D005554:Forensic Medicine / Q000379:methods; D005557:Formaldehyde / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D016612:Paraffin Embedding / Q000379:methods*; D016133:Polymerase Chain Reaction; D013048:Specimen Handling / Q000379:methods; D013997:Time Factors; D016707:Tissue Fixation
D005557:Formaldehyde; D004247:DNA