title: Translocation of alpha-synuclein expressed in Escherichia coli.
abstract: alpha-Synuclein is a major component of Lewy bodies in Parkinson's disease. Although no signal sequence is apparent, alpha-synuclein expressed in Escherichia coli is mostly located in the periplasm. The possibilities that alpha-synuclein translocated into the periplasm across the inner membrane by the SecA or the Tat targeting route identified in bacteria and that alpha-synuclein was released through MscL were excluded. The signal recognition particle-dependent pathway is involved in the translocation of alpha-synuclein. The C-terminal 99-to-140 portion of the alpha-synuclein molecule plays a signal-like role for its translocation into the periplasm, cooperating with the central 61-to-95 section. The N-terminal 1-to-60 region is not required for this translocation.
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China.
authors.forename: Guoping
authors.initials: G
authors.lastname: Ren
authors.forename: Xi
authors.initials: X
authors.lastname: Wang
authors.forename: Shufeng
authors.initials: S
authors.lastname: Hao
authors.forename: Hongyu
authors.initials: H
authors.lastname: Hu
authors.forename: Chih-Chen
authors.initials: CC
authors.lastname: Wang
mesh_terms: D018988:Bacterial Translocation / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D006801:Humans; D010446:Peptide Fragments / Q000378:metabolism; D010957:Plasmids; D021381:Protein Transport; D011994:Recombinant Proteins / Q000378:metabolism; D014357:Trypsin; D051844:alpha-Synuclein / Q000235:genetics / Q000378:metabolism*
chemical_list: D010446:Peptide Fragments; D011994:Recombinant Proteins; D051844:alpha-Synuclein; D014357:Trypsin