In vitro analysis of the two-component system MtrB-MtrA from Corynebacterium glutamicum.
The two-component system MtrBA is involved in the osmostress response of Corynebacterium glutamicum. MtrB was reconstituted in a functionally active form in liposomes and showed autophosphorylation and phosphatase activity. In proteoliposomes, MtrB activity was stimulated by monovalent cations used by many osmosensors for the detection of hypertonicity. Although MtrB was activated by monovalent cations, they lead in vitro to a general stabilization of histidine kinases and do not represent the stimulus for MtrB to sense hyperosmotic stress.
Möker
Nina
N
Institut für Biochemie der Universität zu Köln, Zülpicher Str. 47, D-50674 Köln, Germany.
Krämer
Jens
J
Unden
Gottfried
G
Krämer
Reinhard
R
Morbach
Susanne
S
D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000222:Adaptation, Physiological; D001426:Bacterial Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D002414:Cations, Monovalent / Q000494:pharmacology; D048230:Corynebacterium glutamicum / Q000235:genetics / Q000502:physiology*; D020536:Enzyme Activators / Q000494:pharmacology; D008081:Liposomes; D009997:Osmotic Pressure; D010744:Phosphoric Monoester Hydrolases / Q000187:drug effects / Q000378:metabolism; D010766:Phosphorylation; D016601:RNA-Binding Proteins / Q000187:drug effects / Q000378:metabolism*; D015398:Signal Transduction; D014157:Transcription Factors / Q000187:drug effects / Q000378:metabolism*
D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D002414:Cations, Monovalent; D020536:Enzyme Activators; D008081:Liposomes; C100938:MtrA protein, Bacteria; C079092:MtrB protein, Bacteria; D016601:RNA-Binding Proteins; D014157:Transcription Factors; D010744:Phosphoric Monoester Hydrolases