Characterization and multilineage differentiation of embryonic stem cells derived from a buffalo parthenogenetic embryo.
Embryonic stem (ES) cells derived from mammalian embryos have the ability to form any terminally differentiated cell of the body. We herein describe production of parthenogenetic buffalo (Bubalus Bubalis) blastocysts and subsequent isolation of an ES cell line. Established parthenogenetic ES (PGES) cells exhibited diploid karyotype and high telomerase activity. PGES cells showed remarkable long-term proliferative capacity providing the possibility for unlimited expansion in culture. Furthermore, these cells expressed key ES cell-specific markers defined for primate species including stage-specific embryonic antigen-4 (SSEA-4), tumor rejection antigen-1-81 (TRA-1-81), and octamer-binding transcription factor 4 (Oct-4). In vitro, in the absence of a feeder layer, cells readily formed embryoid bodies (EBs). When cultured for an extended period of time, EBs spontaneously differentiated into derivatives of three embryonic germ layers as detected by PCR for ectodermal (nestin, oligodendrocytes, and tubulin), mesodermal (scleraxis, alpha-skeletal actin, collagen II, and osteocalcin) and endodermal markers (insulin and alpha-fetoprotein). Differentiation of PGES cells toward chondrocyte lineage was directed by supplementing serum-containing media with ascorbic acid, beta-glycerophosphate, and dexamethasone. Moreover, when PGES cells were injected into nude mice, teratomas with derivatives representing all three embryonic germ layers were produced. Our results suggest that the cell line isolated from a parthenogenetic blastocyst holds properties of ES cells, and can be used as an in vitro model to study the effects of imprinting on cell differentiation and as an a invaluable material for extensive molecular studies on imprinted genes.
Sritanaudomchai
Hathaitip
H
Department of Biochemistry, Faculty of science, Mahidol University, Bangkok, Thailand.
Pavasuthipaisit
Kanok
K
Kitiyanant
Yindee
Y
Kupradinun
Piengchai
P
Mitalipov
Shoukhrat
S
Kusamran
Thanit
T
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002020:Buffaloes / Q000196:embryology*; D002454:Cell Differentiation; D019070:Cell Lineage / Q000502:physiology; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000637:transplantation; D051379:Mice; D008819:Mice, Nude; D010312:Parthenogenesis / Q000502:physiology*
D015415:Biomarkers