Intragastric immunization with recombinant Lactobacillus casei expressing flagellar antigen confers antibody-independent protective immunity against Salmonella enterica serovar Enteritidis.
A recombinant Lactobacillus casei expressing a flagellar antigen from Salmonella enterica serovar Enteritidis was constructed and evaluated as a mucosal vaccine. Intragastric immunization of the recombinant strain conferred protective immunity against Salmonella infection in mice. This immunization did not result in antigen-specific antibody in either feces or sera but induced the release of IFN-gamma on restimulation of primed lymphocytes ex vivo. The results suggested that the protective efficacy provided by flagellin-expressing L. casei is mainly attributable to cell-mediated immune responses. In addition, an adjuvant-type effect of the antigen delivery system with L. casei was also observed.
Kajikawa
Akinobu
A
Division of Biomedical Food Research, National Institute of Health Sciences, Tokyo, Japan. kajikawa@nihs.go.jp
Satoh
Eiichi
E
Leer
Rob J
RJ
Yamamoto
Shigeki
S
Igimi
Shizunobu
S
D000284:Administration, Oral; D000818:Animals; D005260:Female; D005408:Flagellin / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007114:Immunization / Q000379:methods; D007371:Interferon-gamma / Q000096:biosynthesis; D007780:Lacticaseibacillus casei / Q000235:genetics*; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D011995:Recombination, Genetic; D012481:Salmonella Infections, Animal / Q000382:microbiology / Q000517:prevention & control*; D022562:Salmonella Vaccines / Q000008:administration & dosage* / Q000276:immunology; D012477:Salmonella enteritidis / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
D022562:Salmonella Vaccines; D005408:Flagellin; D007371:Interferon-gamma