title: Innate-like gammadelta T cell responses to mycobacterium Bacille Calmette-Guerin using the public V gamma 2 repertoire in Macaca fascicularis.
abstract: The V gamma 2 V delta 2 T cell subset responds to Bacille Calmette-Guerin (BCG) immunization in macaques and may be a component of protective immunity against tuberculosis. We characterized the effects of BCG on the V gamma 2 V delta 2 T cell receptor repertoire by comparing the starting population of V gamma 2 chains in cynomolgus macaques with the repertoire found after priming or booster immunization with BCG. The starting repertoire was dominated by public V gamma 2 chain sequences that were found repeatedly among unrelated animals. Primary exposure to BCG triggered expansion of cells expressing public V gamma 2 chains and booster immunization was often associated with contraction of these same subsets. Thus, BCG-reactive V gamma 2 chains were present at high frequency in the repertoire of mycobacteria-naïve macaques and they comprised the major response to primary or booster immunization. Normal selection processes that created the naïve V gamma 2 repertoire in macaques, also encoded the capacity for rapid responses to mycobacteria. The unusual composition of a normal V gamma 2 repertoire helps to explain the powerful gammadelta T cell responses to BCG immunization.
authors.affiliation: University of Maryland School of Medicine, Institute of Human Virology, 725 W Lombard St, Baltimore, MD 21201, USA.
authors.forename: Cristiana
authors.initials: C
authors.lastname: Cairo
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Hebbeler
authors.forename: Nadia
authors.initials: N
authors.lastname: Propp
authors.forename: Joseph L
authors.initials: JL
authors.lastname: Bryant
authors.forename: Vittorio
authors.initials: V
authors.lastname: Colizzi
authors.forename: C David
authors.initials: CD
authors.lastname: Pauza
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial / Q000008:administration & dosage; D001500:BCG Vaccine / Q000276:immunology* / Q000494:pharmacology*; D007114:Immunization; D008168:Lung / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology*; D008252:Macaca fascicularis / Q000276:immunology*; D023421:Models, Animal; D008969:Molecular Sequence Data; D009163:Mycobacterium bovis; D016692:Receptors, Antigen, T-Cell, gamma-delta; D016415:Sequence Alignment; D013045:Species Specificity; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D001500:BCG Vaccine; D016692:Receptors, Antigen, T-Cell, gamma-delta