title: Carboxypeptidases B of Anopheles gambiae as targets for a Plasmodium falciparum transmission-blocking vaccine.
abstract: Anopheles gambiae is the major African vector of Plasmodium falciparum, the most deadly species of human malaria parasite and the most prevalent in Africa. Several strategies are being developed to limit the global impact of malaria via reducing transmission rates, among which are transmission-blocking vaccines (TBVs), which induce in the vertebrate host the production of antibodies that inhibit parasite development in the mosquito midgut. So far, the most promising components of a TBV are parasite-derived antigens, although targeting critical mosquito components might also successfully block development of the parasite in its vector. We previously identified A. gambiae genes whose expression was modified in P. falciparum-infected mosquitoes, including one midgut carboxypeptidase gene, cpbAg1. Here we show that P. falciparum up-regulates the expression of cpbAg1 and of a second midgut carboxypeptidase gene, cpbAg2, and that this up-regulation correlates with an increased carboxypeptidase B (CPB) activity at a time when parasites establish infection in the mosquito midgut. The addition of antibodies directed against CPBAg1 to a P. falciparum-containing blood meal inhibited CPB activity and blocked parasite development in the mosquito midgut. Furthermore, the development of the rodent parasite Plasmodium berghei was significantly reduced in mosquitoes fed on infected mice that had been immunized with recombinant CPBAg1. Lastly, mosquitoes fed on anti-CPBAg1 antibodies exhibited reduced reproductive capacity, a secondary effect of a CPB-based TBV that could likely contribute to reducing Plasmodium transmission. These results indicate that A. gambiae CPBs could constitute targets for a TBV that is based upon mosquito molecules.
authors.affiliation: Unité de Biologie et Génétique du Paludisme, Institut Pasteur, 25 rue du Dr Roux, 75015 Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Lavazec
authors.forename: C
authors.initials: C
authors.lastname: Boudin
authors.forename: R
authors.initials: R
authors.lastname: Lacroix
authors.forename: S
authors.initials: S
authors.lastname: Bonnet
authors.forename: A
authors.initials: A
authors.lastname: Diop
authors.forename: S
authors.initials: S
authors.lastname: Thiberge
authors.forename: B
authors.initials: B
authors.lastname: Boisson
authors.forename: R
authors.initials: R
authors.lastname: Tahar
authors.forename: C
authors.initials: C
authors.lastname: Bourgouin
mesh_terms: D000818:Animals; D000852:Anopheles / Q000201:enzymology / Q000276:immunology / Q000469:parasitology* / Q000502:physiology; D000906:Antibodies / Q000276:immunology; D043424:Carboxypeptidase B / Q000037:antagonists & inhibitors / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D041981:Gastrointestinal Tract / Q000201:enzymology / Q000469:parasitology; D006801:Humans; D008288:Malaria / Q000517:prevention & control / Q000635:transmission; D017780:Malaria Vaccines; D016778:Malaria, Falciparum / Q000517:prevention & control* / Q000635:transmission; D051379:Mice; D010962:Plasmodium berghei / Q000254:growth & development; D010963:Plasmodium falciparum / Q000254:growth & development*; D012098:Reproduction; D015854:Up-Regulation
chemical_list: D000906:Antibodies; D017780:Malaria Vaccines; D043424:Carboxypeptidase B