Prevention of bacterial translocation using beta-(1-3)-D-glucan in small bowel ischemia and reperfusion in rats.
OBJECTIVE
To investigate the role of beta-(1-3)-D-glucan on 99mTc labelled Escherichia coli translocation and cytokines secretion in rats submitted to small bowel ischemia/reperfusion injury.


METHODS
Five groups (n=10 each) of Wistar rats were subjected to control(C), sham(S), group IR subjected to 45 min of bowel ischemia/60 min of reperfusion(I/R), and group I/R+glucan subjected to 45 min of bowel ischemia/60 min of reperfusion(I/R) and injected with 2 mg/Kg intramuscular. Translocation of labelled bacteria to mesenteric lymph nodes, liver, spleen, lung and serum was determined using radioactivity/count and colony forming units/g(CFU/g). Serum TNFalpha, IL-1beta, IL-6, IL-10 were measured by ELISA.


RESULTS
CFU/g and radioactivity/count were higher in I/R than in I/R+glucan rats. In C, S and S+glucan groups, bacteria and radioactivity/count were rarely detected. The I/R+glucan rats had enhancement of IL-10 and suppressed production of serum TNFalpha, IL-1beta and, IL-6, compared to I/R untreated animals.


CONCLUSIONS
The beta-(1-3)-D-glucan modulated the production of pro-inflammatory and anti-inflammatory cytokines during bowel ischemia/reperfusion, and attenuated translocation of labelled bacteria.
Araújo-Filho
Irami
I
Department of Surgery, UFRN, Brazil.
Rêgo
Amália Cínthia Meneses
AC
Pinheiro
Laíza Araújo Mohana
LA
Azevedo
Italo Medeiros
IM
Medeiros
Vítor Brasil
VB
Brandão-Neto
José
J
Medeiros
Aldo Cunha
AC
D000818:Animals; D018988:Bacterial Translocation / Q000187:drug effects* / Q000502:physiology; D015169:Colony Count, Microbial; D016207:Cytokines / Q000096:biosynthesis / Q000378:metabolism; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000502:physiology*; D018836:Inflammation Mediators / Q000502:physiology; D007413:Intestinal Mucosa / Q000382:microbiology / Q000502:physiology; D007421:Intestine, Small / Q000098:blood supply*; D008297:Male; D011509:Proteoglycans; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000382:microbiology / Q000517:prevention & control*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D047071:beta-Glucans / Q000494:pharmacology*
D016207:Cytokines; D018836:Inflammation Mediators; D011509:Proteoglycans; D014409:Tumor Necrosis Factor-alpha; D047071:beta-Glucans; C010770:polysaccharide-K