title: Enhanced heterologous production of eicosapentaenoic acid in Escherichia coli cells that co-express eicosapentaenoic acid biosynthesis pfa genes and foreign DNA fragments including a high-performance catalase gene, vktA.
abstract: Cellular eicosapentaenoic acid (EPA) makes up approximately 3% of total fatty acids in Escherichia coli DH5alpha, a strain that carries EPA biosynthesis genes (pEPADelta1). EPA was increased to 12% of total fatty acids when the host cell co-expressed the vector pGBM3::sa1(vktA), which carried the high-performance catalase gene, vktA. Where this vector was co-expressed, the transformant accumulated a large amount of VktA protein. However, the EPA production of cells carrying the vector, that included the insert lacking almost the entire vktA gene, was approximately 6%. This suggests that the retention of a large DNA insert in the vector and the accumulation of the resulting protein, but not the catalytic activity of VktA catalase, would potentially be able to increase the content of EPA.
authors.affiliation: Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo, 060-0810, Japan.
authors.forename: Yoshitake
authors.initials: Y
authors.lastname: Orikasa
authors.forename: Yukiya
authors.initials: Y
authors.lastname: Ito
authors.forename: Takanori
authors.initials: T
authors.lastname: Nishida
authors.forename: Kazuo
authors.initials: K
authors.lastname: Watanabe
authors.forename: Naoki
authors.initials: N
authors.lastname: Morita
authors.forename: Takuji
authors.initials: T
authors.lastname: Ohwada
authors.forename: Isao
authors.initials: I
authors.lastname: Yumoto
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Okuyama
mesh_terms: D002374:Catalase / Q000235:genetics*; D004247:DNA / Q000378:metabolism*; D015118:Eicosapentaenoic Acid / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D015870:Gene Expression; D005798:Genes, Bacterial; D015183:Restriction Mapping
chemical_list: D004247:DNA; D015118:Eicosapentaenoic Acid; D002374:Catalase