Determination of the systematic and random measurement error in an LC-FTICR mass spectrometry analysis of a partially characterized complex peptide mixture.
In high-throughput proteomics, a promising approach presently being explored is the use of liquid chromatography coupled to Fourier transform ion cyclotron resonance mass spectrometry (LC-FTICR-MS) to provide measurements of the masses of tryptic peptides in complex mixtures, which can then be used to identify the proteins which gave rise to those peptides. In order to apply this method, it is necessary to account for any systematic measurement error, and it is useful to have an estimate of the random error in measured masses. In this investigation, a complex mixture of peptides derived from a partially characterized sample was analyzed by LC-FTICR-MS. Through the application of a Bayesian probability model of the data, partial knowledge of the composition of the sample is sufficient both to determine any systematic error and to estimate the random error in measured masses.
Yanofsky
C M
CM
Dept. of Biomedical Eng., McGill Univ., Que., Canada.
Kearney
R E
RE
Morales
F
F
Lam
T-K T
TK
Blakney
G T
GT
Marshall
A G
AG
Carrillo
B
B
Lekpor
K
K
Boismenu
D
D
Bell
A W
AW