Diffusion and segmental dynamics of double-stranded DNA.
Diffusion and segmental dynamics of the double-stranded lambda-phage DNA polymer are quantitatively studied over the transition range from stiff to semiflexible chains. Spectroscopy of fluorescence fluctuations of single-end fluorescently labeled monodisperse DNA fragments unambiguously shows that double-stranded DNA in the length range of 10(2) - 2 x 10(4) base pairs behaves as a semiflexible polymer with segmental dynamics controlled by hydrodynamic interactions.
Petrov
E P
EP
Institute of Biophysics/BIOTEC, Dresden University of Technology, Tatzberg 47-51, 01307 Dresden, Germany.
Ohrt
T
T
Winkler
R G
RG
Schwille
P
P
D010582:Bacteriophage lambda / Q000378:metabolism*; D004247:DNA / Q000737:chemistry*; D004279:DNA, Viral / Q000737:chemistry*; D004058:Diffusion; D005456:Fluorescent Dyes / Q000737:chemistry; D013057:Spectrum Analysis
D004279:DNA, Viral; D005456:Fluorescent Dyes; D004247:DNA