Composite organic-inorganic nanoparticles as Raman labels for tissue analysis.
Composite organic-inorganic nanoparticles (COINs) are novel optical labels for detection of biomolecules. We have previously developed methods to encapsulate COINs and to functionalize them with antibodies. Here we report the first steps toward application of COINs to the detection of proteins in human tissues. Two analytes, PSA and CK18, are detected simultaneously using two different COINs in a direct binding assay, and two different COINs are shown to simultaneously label PSA in tissue samples.
Sun
Lei
L
Biomedical/Life Sciences, Digital Health Group, Intel Corporation, SC3-41, 2200 Mission College Boulevard, Santa Clara, California 95054, USA.
Sung
Kung-Bin
KB
Dentinger
Claire
C
Lutz
Barry
B
Nguyen
Lienchi
L
Zhang
Jingwu
J
Qin
Haoyu
H
Yamakawa
Mineo
M
Cao
Manqiu
M
Lu
Yu
Y
Chmura
A J
AJ
Zhu
Jing
J
Su
Xing
X
Berlin
Andrew A
AA
Chan
Selena
S
Knudsen
Beatrice
B
D000906:Antibodies; D004797:Enzyme-Linked Immunosorbent Assay; D006651:Histocytochemistry / Q000379:methods; D006801:Humans; D053538:Keratin-18 / Q000032:analysis; D008297:Male; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D011467:Prostate / Q000737:chemistry; D017430:Prostate-Specific Antigen / Q000032:analysis; D011485:Protein Binding; D013059:Spectrum Analysis, Raman
D000906:Antibodies; D053538:Keratin-18; D017430:Prostate-Specific Antigen