title: Optical recording of action potentials and other discrete physiological events: a perspective from signal detection theory.
abstract: Optical imaging of physiological events in real time can yield insights into biological function that would be difficult to obtain by other experimental means. However, the detection of all-or-none events, such as action potentials or vesicle fusion events, in noisy single-trial data often requires a careful balance of tradeoffs. The analysis of such experiments, as well as the design of optical reporters and instrumentation for them, is aided by an understanding of the principles of signal detection. This review illustrates these principles, using as an example action potential recording with optical voltage reporters.
authors.affiliation: Department of Cell Biology, Yale University School of Medicine, New Haven, CT, USA. lukesjulson@gmail.com
authors.forename: Lucas
authors.initials: L
authors.lastname: Sjulson
authors.forename: Gero
authors.initials: G
authors.lastname: Miesenböck
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D018613:Microscopy, Confocal / Q000379:methods*; D008962:Models, Theoretical; D009474:Neurons / Q000502:physiology*; D055095:Optics and Photonics; D017785:Photons