Low dose of Concanavalin-A enhances innate immune response and prevents liver injury in mice infected with Candida albicans.
The mechanisms through which Candida albicans is recognized by immune cells and how it triggers host defence are not completely understood. In this study, we evaluated the effect of Concanavalin-A on the clearance of C. albicans by infected mice and their production of proinflammatory cytokine tumor necrosis factor-alpha (TNF-alpha). Subgroups of 5 animals were pretreated with Con-A (250 mug mL(-1) PBS) and after 96 h were infected intraperitoneally with 10(7) cells of C. albicans CR15 (an isolate from a HIV+ person); 30 min, 2, 6, 24 or 72 h after infection the mice were sacrificed. Phagocytosis of C. albicans by peritoneal macrophages increased 30 min after infection in mice pretreated with Con-A. The liver presented the greatest number of CFUs, and this number was reduced by pretreatment with Con-A. Control animals infected with C. albicans presented a significant increase in plasmatic alanine aminotransferase, which was not observed in mice treated with Con-A. Two hours after infection the production of TNF-alpha in the liver of mice pretreated with Con-A was significantly increased. These results suggest that a single dose of Con-A caused a beneficial modulating action of the inflammatory response during infection with C. albicans.
Conchon-Costa
I
I
Department of Pathological Sciences, Universidade Estadual de Londrina, Londrina, Paraná, Brazil.
Loyola
W
W
Gaziri
L C J
LC
Custódio
L A
LA
Felipe
I
I
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D002176:Candida albicans / Q000254:growth & development / Q000276:immunology*; D002177:Candidiasis / Q000188:drug therapy / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D015169:Colony Count, Microbial; D003208:Concanavalin A / Q000008:administration & dosage*; D004195:Disease Models, Animal; D006651:Histocytochemistry; D007155:Immunologic Factors / Q000008:administration & dosage*; D008099:Liver / Q000276:immunology / Q000382:microbiology* / Q000473:pathology; D017737:Macrophages, Peritoneal / Q000276:immunology; D051379:Mice; D010587:Phagocytosis; D016019:Survival Analysis; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D007155:Immunologic Factors; D014409:Tumor Necrosis Factor-alpha; D003208:Concanavalin A; D000410:Alanine Transaminase