title: Intranasal CpG DNA therapy during allergen exposure in allergic rhinitis.
abstract: OBJECTIVE
  1) To estimate the effectiveness of intranasal administration of CpG DNA alone on allergic rhinitis compared with intradermal administration; and 2) to find out how CpG DNA therapy is useful in treatment of allergic rhinitis.
  
  
  METHODS
  Mice were intraperitoneally sensitized and intranasally challenged with Japanese cedar. Therapy with CpG DNA alone was also performed during challenge, either intranasally or intradermally. Immunologic variables and nasal symptom were studied.
  
  
  RESULTS
  Intranasal administration of CpG DNA alone significantly reduced the levels of IgE, IL-5 productions from nasal lymphocytes and splenocytes, nasal eosinophilia, and nasal symptoms, although intradermal administration of CpG DNA alone showed no significant reduction.
  
  
  CONCLUSIONS
  This study demonstrated that CpG DNA has effects not only on splenocytes but also on nasal lymphocytes to attenuate allergic rhinitis, and that intranasal administration, but not intradermal administration, of CpG DNA alone during allergen exposure is useful for control of allergic rhinitis.
authors.affiliation: Department of Otorhinolaryngology, Graduate School of Medical Sciences, Nagoya City University, Nagoya, Japan. msuzuki5@uwo.ca
authors.forename: Motohiko
authors.initials: M
authors.lastname: Suzuki
authors.forename: Tamami
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Nobuo
authors.initials: N
authors.lastname: Ohta
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Min
authors.forename: Shingo
authors.initials: S
authors.lastname: Murakami
mesh_terms: D000279:Administration, Cutaneous; D000281:Administration, Intranasal; D000284:Administration, Oral; D000485:Allergens / Q000276:immunology*; D000818:Animals; D018899:CpG Islands / Q000276:immunology*; D032485:Cryptomeria / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D004802:Eosinophilia / Q000517:prevention & control; D018984:Epitopes, T-Lymphocyte; D005260:Female; D007073:Immunoglobulin E / Q000276:immunology; D015848:Interleukin-5 / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011058:Pollen / Q000276:immunology*; D012220:Rhinitis / Q000188:drug therapy* / Q000276:immunology
chemical_list: D000485:Allergens; D018984:Epitopes, T-Lymphocyte; D015848:Interleukin-5; D007073:Immunoglobulin E