Real-time PCR assay using specimens on filter disks as a template for detection of cytomegalovirus in urine.
Since congenital cytomegalovirus (CMV) infection causes late-onset sequelae, the identification of CMV-infected newborns is important. For this purpose, we established a simple real-time PCR assay using a filter disk. Combined with the collection of urine using filter papers placed in the diaper, this assay can make CMV screening more feasible and cost-effective.
Nozawa
Naoki
N
Department of Virology I, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
Koyano
Shin
S
Yamamoto
Yumiko
Y
Inami
Yuhki
Y
Kurane
Ichiro
I
Inoue
Naoki
N
D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D016133:Polymerase Chain Reaction / Q000379:methods*; D014556:Urine / Q000821:virology*; D014773:Virology / Q000379:methods*
D004279:DNA, Viral