Induction of human umbilical cord blood-derived stem cells with embryonic stem cell phenotypes into insulin producing islet-like structure.
Success in islet-transplantation-based therapies for type I diabetes, coupled with a worldwide shortage of transplant-ready islets, has motivated efforts to develop renewable sources of islet-replacement tissue. Embryonic stem cells (ESCs) have been successfully induced into insulin producing islet-like structure in several studies. However, the source of the ESCs has presented ethical and technical concerns. Here, we isolated a population of stem cells from human cord blood (UCB), which expressed embryo stage specific maker, SSEA-4, and the multi-potential stem cell marker, Oct4. Subsequently, we successfully induced them into insulin-producing islet-like structures, which co-express insulin and C-peptide. These findings might have a significant potential to advance human UCB derived stem-cell-based therapeutics for diabetes.
Sun
Bo
B
Laboratory of Stem Cell and Tumor Biology, College of Veterinary Medicine, Seoul National University, Seoul, South Korea.
Roh
Kyung-Hwan
KH
Lee
Sae-Rom
SR
Lee
Yong-Soon
YS
Kang
Kyung-Sun
KS
D002096:C-Peptide / Q000096:biosynthesis; D002454:Cell Differentiation / Q000502:physiology; D036101:Cord Blood Stem Cell Transplantation; D053595:Embryonic Stem Cells / Q000502:physiology*; D005312:Fetal Blood / Q000166:cytology / Q000378:metabolism; D006028:Glycosphingolipids / Q000096:biosynthesis; D006801:Humans; D007328:Insulin / Q000096:biosynthesis; D050417:Insulin-Secreting Cells / Q000502:physiology*; D050814:Octamer Transcription Factor-3 / Q000096:biosynthesis; D010641:Phenotype; D055746:Stage-Specific Embryonic Antigens; D013234:Stem Cells / Q000378:metabolism
D002096:C-Peptide; D006028:Glycosphingolipids; D007328:Insulin; D050814:Octamer Transcription Factor-3; D055746:Stage-Specific Embryonic Antigens; C066584:stage-specific embryonic antigen-4