title: [Study on the rapid detection of Dengue viruses by Taqman MGB real-time fluorescent PCR].
abstract: OBJECTIVE
  To develop the Taqman MGB real-time fluorescent PCR assay for rapid detection of Dengue viruses.
  
  
  METHODS
  Using Taqman MGB technique, a pair of universal primers and Taqman MGB probe were designed according to a highly reserved sequence of the 3'-noncoding region of dengue viruses type 1-4. Dengue virus strains were used as standard and Japanese encephalitis virus strains were used as control, the real-time PCR assay for specific and sensitive detection of the dengue viruses was established. While 8 serum specimens of ELISA positive were detected by the RT-PCR and fluorescent PCR.
  
  
  RESULTS
  The sensitivity of real-time PCR was 0.17pg/microl (cDNA)or 10(-5)TCID50. There was no cross-reaction with Japanese encephalitis virus. Of 8 specimens, 2 were positive by RT-PCR and 5 were positive by real-time PCR. The test could be completed in 4 hours.
  
  
  CONCLUSIONS
  The Taqman MGB real-time PCR assay was fast, sensitive and specific. It could be applied to the quick early diagnosis of dengue viruses.
authors.affiliation: Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China.
authors.forename: Jian-jun
authors.initials: JJ
authors.lastname: Liu
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.forename: Jian-fan
authors.initials: JF
authors.lastname: He
authors.forename: Jia-min
authors.initials: JM
authors.lastname: Chen
authors.forename: Ya-qing
authors.initials: YQ
authors.lastname: He
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D003715:Dengue / Q000175:diagnosis / Q000821:virology*; D003716:Dengue Virus / Q000235:genetics / Q000302:isolation & purification*; D005456:Fluorescent Dyes; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D005456:Fluorescent Dyes