title: Pericytes of human skeletal muscle are myogenic precursors distinct from satellite cells.
abstract: Cells derived from blood vessels of human skeletal muscle can regenerate skeletal muscle, similarly to embryonic mesoangioblasts. However, adult cells do not express endothelial markers, but instead express markers of pericytes, such as NG2 proteoglycan and alkaline phosphatase (ALP), and can be prospectively isolated from freshly dissociated ALP(+) cells. Unlike canonical myogenic precursors (satellite cells), pericyte-derived cells express myogenic markers only in differentiated myotubes, which they form spontaneously with high efficiency. When transplanted into severe combined immune deficient-X-linked, mouse muscular dystrophy (scid-mdx) mice, pericyte-derived cells colonize host muscle and generate numerous fibres expressing human dystrophin. Similar cells isolated from Duchenne patients, and engineered to express human mini-dystrophin, also give rise to many dystrophin-positive fibres in vivo. These data show that myogenic precursors, distinct from satellite cells, are associated with microvascular walls in the human skeletal muscle, may represent a correlate of embryonic 'mesoangioblasts' present after birth and may be a promising candidate for future cell-therapy protocols in patients.
authors.affiliation: Stem Cell Research Institute, San Raffaele Scientific Institute, 58 Via Olgettina, 20132 Milan, Italy.
authors.forename: Arianna
authors.initials: A
authors.lastname: Dellavalle
authors.forename: Maurilio
authors.initials: M
authors.lastname: Sampaolesi
authors.forename: Rossana
authors.initials: R
authors.lastname: Tonlorenzi
authors.forename: Enrico
authors.initials: E
authors.lastname: Tagliafico
authors.forename: Benedetto
authors.initials: B
authors.lastname: Sacchetti
authors.forename: Laura
authors.initials: L
authors.lastname: Perani
authors.forename: Anna
authors.initials: A
authors.lastname: Innocenzi
authors.forename: Beatriz G
authors.initials: BG
authors.lastname: Galvez
authors.forename: Graziella
authors.initials: G
authors.lastname: Messina
authors.forename: Roberta
authors.initials: R
authors.lastname: Morosetti
authors.forename: Sheng
authors.initials: S
authors.lastname: Li
authors.forename: Marzia
authors.initials: M
authors.lastname: Belicchi
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Peretti
authors.forename: Jeffrey S
authors.initials: JS
authors.lastname: Chamberlain
authors.forename: Woodring E
authors.initials: WE
authors.lastname: Wright
authors.forename: Yvan
authors.initials: Y
authors.lastname: Torrente
authors.forename: Stefano
authors.initials: S
authors.lastname: Ferrari
authors.forename: Paolo
authors.initials: P
authors.lastname: Bianco
authors.forename: Giulio
authors.initials: G
authors.lastname: Cossu
mesh_terms: D000293:Adolescent; D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D000368:Aged; D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D018929:Cell Culture Techniques / Q000379:methods; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D018101:Mice, Inbred mdx; D008819:Mice, Nude; D016513:Mice, SCID; D008875:Middle Aged; D009124:Muscle Proteins / Q000032:analysis / Q000235:genetics; D018482:Muscle, Skeletal / Q000737:chemistry / Q000166:cytology* / Q000502:physiology; D020388:Muscular Dystrophy, Duchenne / Q000503:physiopathology / Q000601:surgery; D020286:Pericytes / Q000737:chemistry / Q000166:cytology* / Q000637:transplantation; D012038:Regeneration / Q000502:physiology*; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D033581:Stem Cell Transplantation / Q000379:methods; D016896:Treatment Outcome
chemical_list: D015703:Antigens, CD; D009124:Muscle Proteins