Combination treatment with donor interleukin-12p35 silenced dendritic cells and cyclosporine induces long-term survival of intestinal allografts in rats.
Immune tolerance promises to enhance allograft survival while reducing risks inherent to immunosuppressants. In this study, we evaluated the effect of interleukin (IL)-12p35 silenced dendritic cells (DC) combined with cyclosporine (CsA) on induction of immune tolerance in intestinal transplantation. The results showed that combination treatment with donor IL-12p35 silenced DC and a low dose of CsA (8 mg/kg) prolonged rat intestinal allograft survival. However, a higher dose of CsA (20 mg/kg) did not further prolong allograft survival.
Xu
H
H
Department of Gastrointestinal Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
Chen
T
T
Wang
H Q
HQ
Hua
Y B
YB
Wu
W W
WW
D000818:Animals; D003131:Combined Modality Therapy; D016572:Cyclosporine / Q000627:therapeutic use*; D003713:Dendritic Cells / Q000276:immunology* / Q000637:transplantation*; D020868:Gene Silencing; D006085:Graft Survival / Q000276:immunology; D053765:Interleukin-12 Subunit p35 / Q000235:genetics*; D007421:Intestine, Small / Q000637:transplantation*; D007422:Intestines / Q000637:transplantation*; D008297:Male; D023421:Models, Animal; D051381:Rats; D011920:Rats, Inbred WF; D017207:Rats, Sprague-Dawley; D013534:Survival; D014184:Transplantation, Homologous / Q000276:immunology*
D053765:Interleukin-12 Subunit p35; D016572:Cyclosporine