title: Intracellular distribution of TiO2-DNA oligonucleotide nanoconjugates directed to nucleolus and mitochondria indicates sequence specificity.
abstract: Deoxyribonucleic acid (DNA) oligonucleotides hybridize to matching DNA sequences in cells, as established in the literature, depending on active transcription of the target sequence and local molarity of the oligonucleotide. We investigated the intracellular distribution of nanoconjugates composed of DNA oligonucleotides attached to TiO2 nanoparticles, thus creating a locally increased concentration of the oligonucleotide. Two types of nanoconjugates, with oligonucleotides matching mitochondrial or nucleolar DNA, were specifically retained in mitochondria or nucleoli.
authors.affiliation: Department of Radiation Oncology, Northwestern University, Chicago, Illinois 60611, USA.
authors.forename: Tatjana
authors.initials: T
authors.lastname: Paunesku
authors.forename: Stefan
authors.initials: S
authors.lastname: Vogt
authors.forename: Barry
authors.initials: B
authors.lastname: Lai
authors.forename: Jörg
authors.initials: J
authors.lastname: Maser
authors.forename: Natasa
authors.initials: N
authors.lastname: Stojićević
authors.forename: Kenneth T
authors.initials: KT
authors.lastname: Thurn
authors.forename: Clodia
authors.initials: C
authors.lastname: Osipo
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Daniel
authors.initials: D
authors.lastname: Legnini
authors.forename: Zhou
authors.initials: Z
authors.lastname: Wang
authors.forename: Chung
authors.initials: C
authors.lastname: Lee
authors.forename: Gayle E
authors.initials: GE
authors.lastname: Woloschak
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002466:Cell Nucleolus / Q000378:metabolism* / Q000648:ultrastructure; D004272:DNA, Mitochondrial / Q000378:metabolism; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000378:metabolism* / Q000648:ultrastructure; D036103:Nanotechnology; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000378:metabolism*; D016716:PC12 Cells; D051381:Rats; D014025:Titanium / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D004272:DNA, Mitochondrial; D009838:Oligodeoxyribonucleotides; C009495:titanium dioxide; D014025:Titanium