title: Molecular cloning and functional expression of soybean allene oxide synthases.
abstract: A plant allene oxide synthase (AOS) reacting with 13S-hydroperoxy-9Z,11E,15Z-octadecatrienoic acid (13-HPOT), a lipoxygenase product of alpha-linolenic acid, provides an allene oxide which functions as an intermediate for jasmonic acid (JA) synthesis, making AOS a key enzyme regulating the JA level in plants. Although AOSs in various plants have been investigated, there is only limited information about AOSs in soybean (Glycine max). In this study, we cloned and characterized two soybean AOSs, GmAOS1 and GmAOS2, sharing 95% homology in the predicted amino acid sequences. GmAOS1 and GmAOS2 were composed of 564 and 559 amino acids respectively, with predicted N-terminal chloroplast-targeting signal peptides. Both AOSs expressed in Escherichia coli were selective for 13S-hydroperoxides of alpha-linolenic and linoleic acids, suggesting the potential of GmAOS1 and GmAOS2 to contribute to JA synthesis. GmAOS1 and GmAOS2 were expressed in leaves, stems, and roots, suggesting broad distribution in a soybean plant.
authors.affiliation: Division of Applied Resources Chemistry, The United Graduate School of Agricultural Sciences, Tottori University, Koyama-minami, Tottori, Japan.
authors.forename: Darika
authors.initials: D
authors.lastname: Kongrit
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Jisaka
authors.forename: Chitose
authors.initials: C
authors.lastname: Iwanaga
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yokomichi
authors.forename: Takuya
authors.initials: T
authors.lastname: Katsube
authors.forename: Kohji
authors.initials: K
authors.lastname: Nishimura
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Nagaya
authors.forename: Kazushige
authors.initials: K
authors.lastname: Yokota
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D005784:Gene Amplification; D019746:Intramolecular Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics*; D007700:Kinetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D018515:Plant Leaves / Q000201:enzymology; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics; D018517:Plant Roots / Q000201:enzymology; D018547:Plant Stems / Q000201:enzymology; D018749:RNA, Plant / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000201:enzymology; D013025:Glycine max / Q000201:enzymology* / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D018749:RNA, Plant; D019746:Intramolecular Oxidoreductases; C021377:hydroperoxide isomerase