title: The formation mechanism by yeast of 4-hydroxy-2(or 5)-ethyl-5(or 2)-methyl-3(2H)-furanone in Miso.
abstract: The mechanism of the formation of 4-hydroxy-2(or 5)-ethyl-5-(or 2)-methyl-3(2H)-furanone (HEMF) with yeast under caltivation in a medium containing amino-carbonyl reactants of ribose and glycine was investigated using stable isotopes of the corresponding compounds. It was confirmed that the skeleton of the five-membered ring and the methyl group of the side chain of HEMF was formed from ribose, and that the ethyl group was derived from the glucose metabolite by yeast. The formation of HEMF was confirmed when acetaldehyde as the glucose metabolite and a cell-free extract from yeast were added to the medium containing amino-carbonyl reactants. These results suggest that the role of yeast in HEMF formation is not only to provide the glucose metabolite, but also in combining the amino-carbonyl reactants with the glucose metabolite.
authors.affiliation: Faculty of Education, Iwate University, Moreoka, Japan.
authors.forename: Motoko
authors.initials: M
authors.lastname: Ohata
authors.forename: Keiko
authors.initials: K
authors.lastname: Kohama
authors.forename: Yasujiro
authors.initials: Y
authors.lastname: Morimitsu
authors.forename: Kikue
authors.initials: K
authors.lastname: Kubota
authors.forename: Etsuko
authors.initials: E
authors.lastname: Sugawara
mesh_terms: D000079:Acetaldehyde / Q000378:metabolism; D002474:Cell-Free System; D003470:Culture Media; D005663:Furans / Q000737:chemistry / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D005947:Glucose / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D007202:Indicators and Reagents; D012266:Ribose / Q000378:metabolism; D045730:Soy Foods / Q000032:analysis*; D020068:Zygosaccharomyces / Q000254:growth & development / Q000378:metabolism*
chemical_list: D003470:Culture Media; D005663:Furans; D007202:Indicators and Reagents; D012266:Ribose; D000079:Acetaldehyde; D005947:Glucose; D005998:Glycine