title: Electrospun PHBV/collagen composite nanofibrous scaffolds for tissue engineering.
abstract: Electrospinning has recently emerged as a leading technique for the formation of nanofibrous structures made of synthetic and natural extracellular matrix components. In this study, nanofibrous scaffolds were obtained by electrospinning a combination of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and type-I collagen in 1,1,1,3,3,3-hexafluoro-2-isopropanol (HIFP). The resulting fibers ranged from 300 to 600 nm in diameter. Their surfaces were characterized by attenuated total reflection Fourier transform infrared spectroscopy, electron spectroscopy for chemical analysis and atomic force microscopy. The PHBV and collagen components of the PHBV/collagen nanofibrous scaffold were biodegraded by PHB depolymerase and a type-I collagenase aqueous solution, respectively. The cell culture experiments indicated that the PHBV/collagen nanofibrous scaffold accelerated the adhesion and growth of NIH3T3 cells more effectively than the PHBV nanofibrous scaffold, thus making the former a good scaffold for tissue engineering.
authors.affiliation: Department of Polymer Science, Kyungpook National University, Daegu 702-701, South Korea.
authors.forename: Wan
authors.initials: W
authors.lastname: Meng
authors.forename: Se-Yong
authors.initials: SY
authors.lastname: Kim
authors.forename: Jiang
authors.initials: J
authors.lastname: Yuan
authors.forename: Jung Chul
authors.initials: JC
authors.lastname: Kim
authors.forename: Oh Hyeong
authors.initials: OH
authors.lastname: Kwon
authors.forename: Naoki
authors.initials: N
authors.lastname: Kawazoe
authors.forename: Guoping
authors.initials: G
authors.lastname: Chen
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ito
authors.forename: Inn-Kyu
authors.initials: IK
authors.lastname: Kang
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D049109:Cell Proliferation; D024042:Collagen Type I / Q000737:chemistry*; D017364:Collagenases / Q000378:metabolism; D051379:Mice; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D041681:NIH 3T3 Cells; D049329:Nanostructures; D011091:Polyesters / Q000737:chemistry*; D020005:Propanols; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D000094122:Polyhydroxybutyrates; D006845:Hydrocarbons, Fluorinated
chemical_list: D024042:Collagen Type I; D017364:Collagenases; D011091:Polyesters; D020005:Propanols; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C001337:hexafluoroisopropanol; D006845:Hydrocarbons, Fluorinated; C417249:collagenase 1