In vitro and in vivo effects of recombinant bovine interferon-tau on bovine leukemia virus.
The antiviral effects of recombinant bovine interferon-tau (rboIFN-tau) on bovine leukemia virus (BLV) were examined in vitro and in vivo. In the in vitro experiments, BLV titers decreased in FLK-BLV cells and in peripheral blood mononuclear cells of BLV-infected cattle treated with rboIFN-tau at a concentration higher than 10(2) U/ml. In order to examine the in vivo effects of rboIFN-tau, 10 BLV-infected cattle were subcutaneously injected with rboIFN-tau. In the first experiment, 6 cows were administrated with 10(5) U/kg body weight of rboIFN-tau 3 times per week for 4 weeks, while in the second experiment 4 cows were administrated with 10(6) U/kg body weight of rboIFN-tau 3 times per week for 3 weeks. No adverse effects were observed after the administration of rboIFN-tau. In experiment No. 1, the mean BLV titers in cattle decreased in the post-rboIFN-tau administration period compared to the pre-rboIFN-tau administration period. In experiment No. 2, the mean BLV titers in cattle decreased in the rboIFN-tau administration period. These results suggest that rboIFN-tau decreases BLV titers in vitro and in vivo and that rboIFN-tau possibly reduces the degree of BLV titer in cattle without severe side effects.
Kohara
Junko
J
Hokkaido Animal Research Center, Shintoku, Japan.
Yokomizo
Yuichi
Y
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology* / Q000627:therapeutic use*; D002417:Cattle; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D016583:Enzootic Bovine Leukosis / Q000188:drug therapy*; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D015726:Giant Cells / Q000187:drug effects; D007370:Interferon Type I / Q000494:pharmacology* / Q000627:therapeutic use*; D001909:Leukemia Virus, Bovine / Q000187:drug effects*; D011257:Pregnancy Proteins / Q000494:pharmacology* / Q000627:therapeutic use*; D011994:Recombinant Proteins
D000998:Antiviral Agents; D007370:Interferon Type I; D011257:Pregnancy Proteins; D011994:Recombinant Proteins; C020947:interferon tau