[Cytotoxicity study of vascular endothelial cells by different kinds of arsenicals].
OBJECTIVE
To observe the cytotoxicity of bovine aortic endothelial cells (BAEC) by different kinds of arsenicals.


METHODS
Cultured BAEC was exposed to arsenite (iAsIII , 0 - 50 micromol/L), arsenate (iAsV ,0 - 10mmol/L) or monomethylarsonous acid (MMAH III, 0 - 2micromol/L) for 24h, respectively. MTT assay and ICP-MS method were used to evaluate the cell viability and the total arsenic concentration (tAs) of BAEC, respectively.


RESULTS
iAsm , iAsV and MMAIII could all decrease the cell viability in a dose-dependent manner (P <0.01). The IC50 values for iAsm , iAsV and MMAIII were determined to be approximately 24.1 micromol/L, 155.9 micromol/L and 1.71 micromol/L, respectively. The order of cytoxicity in BAEC was MMAIII > iAsIII > iAsV . The take-up rate of MMA' by BAEC was significantly higher than iAsR exposure (P < 0.01).


CONCLUSIONS
MMAIII , one of the active intermediate in inorganic arsenic methylation, is highly toxic in BAEC. Methylation of arsenic could be more like a bioactivation process rather than a detoxification process.
Li
Bing
B
Department of Occupational Health, School of Public Health, China Medical University, Shenyang 110001, China.
Lu
Chun-wei
CW
Sun
Gui-fan
GF
D000818:Animals; D001011:Aorta / Q000166:cytology; D001149:Arsenates / Q000633:toxicity*; D001151:Arsenic / Q000032:analysis; D018053:Arsenites / Q000633:toxicity*; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000166:cytology*; D009942:Organometallic Compounds / Q000633:toxicity*
D001149:Arsenates; D018053:Arsenites; D009942:Organometallic Compounds; C406082:monomethylarsonous acid; C015001:arsenite; D001151:Arsenic; C025657:arsenic acid