title: Participation of dihydrostyryl-2-pyrones and styryl-2-pyrones in the central effects of Polygala sabulosa (Polygalaceae), a folk medicine topical anesthetic.
abstract: This study was undertaken to evaluate the psychopharmacological effects in mice of the hydroethanolic extract (HE), aqueous, hexane and ethyl acetate (EA) fractions, and 6-methoxy-7-prenyloxycoumarin, three dihydrostyryl-2-pyrones and three styryl-2-pyrones isolated from Polygala sabulosa (Polygalaceae), a folk medicine used as a topical anesthetic. In the elevated plus-maze test (EPM), the HE of P. sabulosa and its EA induced an increase in the percentage of time spent on, and in the frequency of entries into the open arms, as well as in the number of unprotected head-dipping, besides a reduction in protected stretch-attend postures, thus indicating an anxiolytic-like profile of action for this plant species. In the hypnosis test, HE and EA enhanced the duration of pentobarbital-induced sleep, a hypnosedative effect confirmed in ethyl ether-induced hypnosis. Moreover, both preparations reduced the duration of the first convulsion induced by pentylenetetrazol, besides decreasing the severity of the seizures. The dihydrostyryl-2-pyrones (1) and (3) as well as styryl-2-pyrones (4) and (7), centrally administered, showed a similar anxiolytic-like effect in the EPM test, while the dihydrostyryl-2-pyrone (2) and styryl-2-pyrone (5) were inactive at the doses used here. These results suggest that P. sabulosa is a herbal medicine which possesses anxiolytic-like, hypnosedative and anticonvulsant effects, and these central effects can be attributed to the presence of the dihydrostyryl-2-pyrone and styryl-2-pyrone compounds.
authors.affiliation: Laboratory of Neuropharmacology, Department of Pharmacology, Universidade Federal de Santa Catarina, 88049-900, Florianópolis, SC, Brazil.
authors.forename: Filipe Silveira
authors.initials: FS
authors.lastname: Duarte
authors.forename: Marcelo
authors.initials: M
authors.lastname: Duzzioni
authors.forename: Beatriz Garcia
authors.initials: BG
authors.lastname: Mendes
authors.forename: Moacir Geraldo
authors.initials: MG
authors.lastname: Pizzolatti
authors.forename: Thereza Christina Monteiro
authors.initials: TC
authors.lastname: De Lima
mesh_terms: D018685:Anesthetics, Inhalation / Q000494:pharmacology; D000779:Anesthetics, Local / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology; D001007:Anxiety / Q000523:psychology; D003292:Convulsants; D004149:Dioxoles / Q000737:chemistry / Q000494:pharmacology; D000431:Ethanol; D004986:Ether; D006586:Hexanes; D006993:Hypnotics and Sedatives / Q000494:pharmacology; D007276:Injections, Intraventricular; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D010424:Pentobarbital / Q000494:pharmacology; D010433:Pentylenetetrazole; D010936:Plant Extracts / Q000494:pharmacology; D031801:Polygala / Q000737:chemistry*; D004856:Postural Balance / Q000187:drug effects; D011753:Pyrones / Q000737:chemistry / Q000494:pharmacology*; D012640:Seizures / Q000139:chemically induced / Q000517:prevention & control; D012890:Sleep / Q000187:drug effects; D012997:Solvents; D013343:Styrenes / Q000737:chemistry / Q000494:pharmacology*; D014867:Water
chemical_list: D018685:Anesthetics, Inhalation; D000779:Anesthetics, Local; D014151:Anti-Anxiety Agents; D003292:Convulsants; D004149:Dioxoles; D006586:Hexanes; D006993:Hypnotics and Sedatives; D010936:Plant Extracts; D011753:Pyrones; D012997:Solvents; D013343:Styrenes; D014867:Water; D004986:Ether; D000431:Ethanol; D010424:Pentobarbital; D010433:Pentylenetetrazole