Active immunization against Alzheimer's beta-amyloid peptide using phage display technology.
Beta-amyloid peptide (AbetaP) aggregation, an essentially early event in Alzheimer's disease (AD) pathogenesis, became a target for therapeutics. We developed an immunization procedure, using as antigen the EFRH peptide located at the N-terminal region of AbetaP displayed on the filamentous phage, to raise anti-beta-amyloid peptide antibodies. Data demonstrated that a bacteriophage displaying different numbers of copies of self-epitope modulates the intensity of the immuno response. This immunization procedure led to improvement in cognitive functions and alleviated amyloid pathology in a transgenic mouse (Tg) model of AD.
Solomon
Beka
B
Department of Molecular Microbiology & Biotechnology, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Ramat Aviv, Tel-Aviv 69978, Israel. beka@post.tau.ac.il
D000544:Alzheimer Disease / Q000276:immunology / Q000378:metabolism / Q000517:prevention & control*; D016229:Amyloid beta-Peptides / Q000276:immunology*; D000818:Animals; D001435:Bacteriophages / Q000235:genetics / Q000276:immunology; D000939:Epitopes / Q000008:administration & dosage* / Q000737:chemistry; D005821:Genetic Techniques; D051379:Mice; D008822:Mice, Transgenic; D019151:Peptide Library; D014611:Vaccination
D016229:Amyloid beta-Peptides; D000939:Epitopes; D019151:Peptide Library