title: Leukocyte polycyclic aromatic hydrocarbon-DNA adduct formation and colorectal adenoma.
abstract: Consumption of charbroiled red meat and meat-derived polycyclic aromatic hydrocarbons (PAHs) has been associated with risk of colorectal adenoma, a precursor of colorectal cancer. Furthermore, leukocyte PAH-DNA adduct levels have been demonstrated to increase in response to charbroiled red meat intake but to date there have been no studies that have investigated the relationship between leukocyte PAH-DNA adduct levels and risk of colorectal adenoma. We investigated the relation of leukocyte PAH-DNA adduct formation and colorectal adenoma in a clinic-based case-control study of colorectal adenomas. The study comprised 82 cases of colorectal adenoma and 111 polyp-free controls, none of whom were current smokers. Leukocyte PAH-DNA adducts were measured by a sensitive chemiluminescence immunoassay using an antiserum elicited against DNA modified with (+/-)-7beta,8alpha-dihydroxy-9alpha,10alpha-epoxy-7,8,9,10-tetrahydro-benzo[a]pyrene that recognizes several PAHs bound to human DNA. Leukocyte PAH-DNA adduct levels were higher among colorectal adenoma cases (median, 1.4 adducts per 10(8) nucleotides) than polyp-free controls (median, 1.2 adducts per 10(8) nucleotides) (P = 0.02). There was a positive association between PAH-DNA adduct level and adenoma prevalence: each unit increase in PAH-DNA adduct level (per 10(8) nucleotides) was associated with an odds ratio (OR) of 1.5 [95% confidence interval (CI), 1.1-2.2]. In addition, a comparison of the lowest quartile for PAH-DNA adduct level with the highest quartile yielded an OR of 2.8 (95% CI, 1.2-6.5; P(trend) = 0.048) for risk of colorectal adenoma. These data support a link between PAH exposure and colorectal adenoma.
authors.affiliation: Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Division of Health and Human Services, Bethesda, MD 20852, USA. mgunter@aecom.yu.edu
authors.forename: Marc J
authors.initials: MJ
authors.lastname: Gunter
authors.forename: Rao L
authors.initials: RL
authors.lastname: Divi
authors.forename: Martin
authors.initials: M
authors.lastname: Kulldorff
authors.forename: Roel
authors.initials: R
authors.lastname: Vermeulen
authors.forename: Kathryn J
authors.initials: KJ
authors.lastname: Haverkos
authors.forename: Maryanne M
authors.initials: MM
authors.lastname: Kuo
authors.forename: Paul
authors.initials: P
authors.lastname: Strickland
authors.forename: Miriam C
authors.initials: MC
authors.lastname: Poirier
authors.forename: Nathaniel
authors.initials: N
authors.lastname: Rothman
authors.forename: Rashmi
authors.initials: R
authors.lastname: Sinha
mesh_terms: D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000378:metabolism; D000236:Adenoma / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D000368:Aged; D002273:Carcinogens / Q000378:metabolism*; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000378:metabolism*; D018736:DNA Adducts / Q000097:blood* / Q000276:immunology; D005260:Female; D006801:Humans; D007118:Immunoassay; D007417:Intestinal Polyps / Q000378:metabolism; D007962:Leukocytes / Q000378:metabolism*; D049449:Luminescence; D008297:Male; D008875:Middle Aged; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*
chemical_list: D002273:Carcinogens; D018736:DNA Adducts; D011084:Polycyclic Aromatic Hydrocarbons; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide