[The mechanism of ginsenosides Rg1 and Rb1 promoting glutamic acid release from PC12 cells].
OBJECTIVE
To study the mechanism of ginsenosides Rg1 and Rb1 promoting glutamic acid release from PC12 cells.


METHODS
The amount of glutamic acid released from PC12 cells was measured by high performance liquid chromatography (HPLC). The effect of Rg1 and Rb1 on the phosphorylation of synapsins was detected with immunofluorescent staining and Western blotting.


RESULTS
Both Rg1 (10 micromol x L(-1)) and Rb1 (10 micromol x L(-1)) increased glutamic acid release from PC12 cells. The release of glutamic acid was decreased by pre-incubating with the PKA inhibitor H89. H89 inhibited the release of glutamic acid induced by Rb1, but had no effect on the release of glutamic acid induced by Rg1. Moreover, Rb1 enhanced the phosphorylation of synapsins via PKA pathway, Rg1 was out of touch with this.


CONCLUSIONS
Rb1 may promote release of neurotransmitters by increasing the phosphorylation of synapsins via PKA pathway, whereas the up-regulation of neurotransmitters release induced by Rg1 is independent of the phosphorylation of synapsins.
Xue
Jian-Fei
JF
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Hu
Jin-Feng
JF
Liu
Zhi-Jun
ZJ
Chen
Hong
H
Zhang
Jun-Tian
JT
Chen
Nai-Hong
NH
D000818:Animals; D015153:Blotting, Western; D017868:Cyclic AMP-Dependent Protein Kinases / Q000502:physiology; D005455:Fluorescent Antibody Technique; D036145:Ginsenosides / Q000494:pharmacology*; D018698:Glutamic Acid / Q000378:metabolism*; D007546:Isoquinolines / Q000494:pharmacology; D018377:Neurotransmitter Agents / Q000378:metabolism*; D016716:PC12 Cells; D010766:Phosphorylation; D051381:Rats; D013449:Sulfonamides / Q000494:pharmacology; D016704:Synapsins / Q000378:metabolism
D036145:Ginsenosides; D007546:Isoquinolines; D018377:Neurotransmitter Agents; D013449:Sulfonamides; D016704:Synapsins; D018698:Glutamic Acid; C442759:ginsenoside Rb1; D017868:Cyclic AMP-Dependent Protein Kinases; C063509:N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide; C035054:ginsenoside Rg1