title: Osteoprogenitor response to low-adhesion nanotopographies originally fabricated by electron beam lithography.
abstract: It is considered that cells can use filopodia, or microspikes, to locate sites suitable for adhesion. This has been investigated using a number of mature cell types, but, to our knowledge, not progenitor cells. Chemical and topographical cues on the underlying substrate are a useful tool for producing defined features for cells to respond to. In this study, arrays of nanopits with different symmetries (square or hexagonal arrays with 120 nm diameters, 300 nm center-centre spacings) and osteoprogenitor cells were considered. The pits were fabricated by ultra-high precision electron-beam lithography and then reproduced in polycarbonate by injection moulding with a nickel stamp. Using scanning electron and fluorescence microscopies, the initial interactions of the cells via filopodia have been observed, as have subsequent adhesion and cytoskeletal formation. The results showed increased filopodia interaction with the surrounding nanoarchitecture leading to a decrease in cell spreading, focal adhesion formation and cytoskeletal organisation.
authors.affiliation: Centre for Cell Engineering, Division of Infection and Immunity, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, UK.
authors.forename: Andrew
authors.initials: A
authors.lastname: Hart
authors.forename: Nikolaj
authors.initials: N
authors.lastname: Gadegaard
authors.forename: Chris D W
authors.initials: CD
authors.lastname: Wilkinson
authors.forename: Richard O C
authors.initials: RO
authors.lastname: Oreffo
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Dalby
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology*; D000368:Aged; D000369:Aged, 80 and over; D001854:Bone Marrow Cells / Q000166:cytology*; D002448:Cell Adhesion; D005260:Female; D006801:Humans; D049329:Nanostructures; D011554:Pseudopodia / Q000502:physiology