Solid-phase synthesis and on-column deprotection of RNA from 2'- (and 3'-) O-levulinated (Lv) ribonucleoside monomers.
[reaction: see text] The solid-phase synthesis of oligoribonucleotides derived from ribonucleosides esterified at the 2'- (or 3'-) position with the levulinyl (Lv) group is described. The oligomers can be released from the solid support as 2'-O-Lv ester derivatives or fully deprotected while still attached to the solid support.
Lackey
Jeremy G
JG
Department of Chemistry, McGill University, Montreal, QC, Canada.
Sabatino
David
D
Damha
Masad J
MJ
D002851:Chromatography, High Pressure Liquid; D015870:Gene Expression; D020868:Gene Silencing; D007982:Levulinic Acids / Q000737:chemistry*; D015394:Molecular Structure; D012313:RNA / Q000138:chemical synthesis* / Q000737:chemistry; D012263:Ribonucleosides / Q000737:chemistry*
D007982:Levulinic Acids; D012263:Ribonucleosides; D012313:RNA