Characterization of class I Newcastle disease virus isolates from Hong Kong live bird markets and detection using real-time reverse transcription-PCR.
Newcastle disease viruses isolated from Hong Kong live bird markets (LBMs) were not detected by a USDA-validated matrix gene real-time reverse transcription-PCR (RT-PCR) assay. Based upon phylogenetic analysis of the fusion gene, these viruses were related to lentogenic class I viruses found in U.S. LBMs and wild waterfowl. An alternative real-time RT-PCR assay which complements the matrix gene assay was developed to efficiently detect class I viruses.
Kim
L Mia
LM
Southeast Poultry Research Laboratory, USDA-ARS, 934 College Station Rd., Athens, GA 30605, USA.
King
Daniel J
DJ
Suarez
David L
DL
Wong
Chun W
CW
Afonso
Claudio L
CL
D000818:Animals; D001717:Birds; D006723:Hong Kong; D008969:Molecular Sequence Data; D009521:Newcastle Disease / Q000821:virology*; D009522:Newcastle disease virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D014760:Viral Fusion Proteins / Q000235:genetics
D012367:RNA, Viral; D014760:Viral Fusion Proteins