title: [Detection of urothelial carcinoma of the urinary bladder by multicolor fluorescence in situ hybridization].
abstract: OBJECTIVE
  Urothelial carcinoma of the urinary bladder is a common malignant neoplasm of the genitourinary system. This study was to analyze chromosome aberrations in urothelial carcinoma of the urinary bladder in Chinese, and evaluate the possibility and validity of multicolor fluorescence in situ hybridization (M-FISH) in detecting urothelial carcinoma of the urinary bladder.
  
  
  METHODS
  The probes of chromosome 3, 7, 17 centromeres and 9p21 region were labeled by random primer method. M-FISH was performed on interphase nuclei of 57 samples of urothelial carcinoma to analyze the correlations of chromosome aberration to diagnosis and pathology of urothelial carcinoma.
  
  
  RESULTS
  The rate of aneuploidy was 47.4% for chromosome 3, 50.9% for chromosome 7, 56.1% for chromosome 17, and 59.6% for chromosome 9p21 in urothelial carcinoma. All the aberrations had no correlations to tumor stage. The aberrations of chromosomes 3, 7, 17 were significantly correlated to pathologic grade (P<0.01). As using the 4 chromosome probes in combination, the sensitivity for detecting urothelial carcinoma of the urinary bladder was 54.4%.
  
  
  CONCLUSIONS
  M-FISH may be helpful for detecting urothelial carcinoma of the urinary bladder.
authors.affiliation: Department of Histology and Embryology, Anhui Medical University,Hefei, Anhui, PR China.
authors.forename: Ye-Gui
authors.initials: YG
authors.lastname: Zhang
authors.forename: Xin-Gang
authors.initials: XG
authors.lastname: Bi
authors.forename: Ya-Ling
authors.initials: YL
authors.lastname: Han
authors.forename: Yan
authors.initials: Y
authors.lastname: Cai
authors.forename: Xin
authors.initials: X
authors.lastname: Xu
authors.forename: Yu-Peng
authors.initials: YP
authors.lastname: Wu
authors.forename: Yi-Ling
authors.initials: YL
authors.lastname: Yang
authors.forename: Jian-Hui
authors.initials: JH
authors.lastname: Ma
authors.forename: Ping
authors.initials: P
authors.lastname: Zhao
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Jia
authors.forename: Ming-Rong
authors.initials: MR
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000782:Aneuploidy; D002295:Carcinoma, Transitional Cell / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D002869:Chromosome Aberrations; D002886:Chromosomes, Human, Pair 17; D002893:Chromosomes, Human, Pair 3; D002897:Chromosomes, Human, Pair 7; D002899:Chromosomes, Human, Pair 9; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology