title: Fine mapping of Ahl3 affecting both age-related and noise-induced hearing loss.
abstract: A region in the vicinity of D17Mit119 on mouse chromosome 17 harbors a susceptibility gene, designated as Ahl3, to age-related hearing loss (AHL). We produced congenic lines of C57BL/6 background that substituted regions around D17Mit119 with MSM-derived ones, and examined auditory brainstem response (ABR) thresholds for their hearing capacity at 6 and 12months of age. Three congenic lines carrying the approximately 14-Mb region between D17Mit274 and D17Mit183 retained normal hearing at 12months of age whereas two congenic lines not carrying this region tended to lose hearing at that age. We also investigated noise-induced hearing loss (NIHL) in congenic lines at 1, 7 and 14days after exposure to the noise of 100dB for 1h. Most congenic mice carrying the 14-Mb region did not exhibit permanent threshold shift (PTS) whereas mice not carrying this region exhibited a strong tendency of PTS, indicating the role of Ahl3 in susceptibility to NIHL. These results indicate that Ahl3 exists within the 14-Mb region and affects not only AHL but also NIHL.
authors.affiliation: Department of Molecular Genetics, Niigata University Graduate School of Medical and Dental Sciences, Asahimachi 1-757, Niigata 951-8510, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Morita
authors.forename: Sachiko
authors.initials: S
authors.lastname: Hirokawa
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kikkawa
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Nomura
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Yonekawa
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Shiroishi
authors.forename: Sugata
authors.initials: S
authors.lastname: Takahashi
authors.forename: Ryo
authors.initials: R
authors.lastname: Kominami
mesh_terms: D000375:Aging / Q000235:genetics / Q000502:physiology*; D000818:Animals; D015820:Cadherins / Q000235:genetics*; D002874:Chromosome Mapping; D003051:Cochlea / Q000473:pathology / Q000648:ultrastructure; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D016057:Evoked Potentials, Auditory, Brain Stem / Q000502:physiology*; D005796:Genes / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D034381:Hearing Loss / Q000235:genetics* / Q000503:physiopathology; D006317:Hearing Loss, Noise-Induced / Q000235:genetics* / Q000503:physiopathology; D051379:Mice; D008855:Microscopy, Electron, Scanning; D014851:Wakefulness
chemical_list: D015820:Cadherins; C499091:Cdh23 protein, mouse; D004247:DNA