title: In vivo bioluminescence tumor imaging of RGD peptide-modified adenoviral vector encoding firefly luciferase reporter gene.
abstract: OBJECTIVE
  The goal of this study is to demonstrate the feasibility of chemically modified human adenovirus (Ad) vectors for tumor retargeting.
  
  
  METHODS
  E1- and E3-deleted Ad vectors carrying firefly luciferase reporter gene under cytomegalovirus promoter (AdLuc) was surface-modified with cyclic arginine-glycine-aspartic acid (RGD) peptides through a bifunctional poly(ethyleneglycol) linker (RGD-PEG-AdLuc) for integrin alpha(v)beta(3) specific delivery. The Coxsackie and adenovirus viral receptor (CAR) and integrin alpha(v)beta(3) expression in various tumor cell lines was determined by reverse transcriptase PCR and fluorescence-activated cell sorting. Bioluminescence imaging was performed in vitro and in vivo to evaluate RGD-modified AdLuc infectivity.
  
  
  RESULTS
  RGD-PEG-AdLuc abrogated the native CAR tropism and exhibited significantly enhanced transduction efficiency of integrin-positive tumors than AdLuc through intravenous administration.
  
  
  CONCLUSIONS
  This approach provides a robust platform for site-specific gene delivery and noninvasive monitoring of the transgene delivery efficacy and homing.
authors.affiliation: Department of Radiation Oncology, Stanford University School of Medicine, Stanford, CA, USA.
authors.forename: Gang
authors.initials: G
authors.lastname: Niu
authors.forename: Zhengming
authors.initials: Z
authors.lastname: Xiong
authors.forename: Zhen
authors.initials: Z
authors.lastname: Cheng
authors.forename: Weibo
authors.initials: W
authors.lastname: Cai
authors.forename: Sanjiv S
authors.initials: SS
authors.lastname: Gambhir
authors.forename: Lei
authors.initials: L
authors.lastname: Xing
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Chen
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D045744:Cell Line, Tumor; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D017931:DNA Primers / Q000235:genetics; D005260:Female; D015870:Gene Expression; D017930:Genes, Reporter; D005822:Genetic Vectors; D006801:Humans; D039302:Integrin alphaVbeta3 / Q000235:genetics; D049409:Luciferases, Firefly / Q000235:genetics*; D008163:Luminescent Measurements; D008325:Mammary Neoplasms, Experimental / Q000175:diagnosis / Q000235:genetics; D051379:Mice; D008819:Mice, Nude; D009842:Oligopeptides / Q000235:genetics; D011092:Polyethylene Glycols; D011991:Receptors, Virus / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics; D014161:Transduction, Genetic
chemical_list: C568771:CLMP protein, human; C568772:CLMP protein, mouse; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D017931:DNA Primers; D039302:Integrin alphaVbeta3; D009842:Oligopeptides; D011991:Receptors, Virus; D011994:Recombinant Proteins; D011092:Polyethylene Glycols; C047981:arginyl-glycyl-aspartic acid; D049409:Luciferases, Firefly