[Analyzing the time course of changes in the titers of specific antibodies while immunization of animals with the biological agent EgFB with different adjuvants].
The recombinant Echinococcus antigens EgF and EgB were prepared and purified by gel filtration and ion-exchange chromatography (800 and 780 mg, respectively being obtained). Four vaccinating complexes, the major component of which were the recombinant antigens EgF and EgB, were developed and tested in an experiment on rabbits. The time course of changes in antibody genesis was studied during a single immunization of the animals with the biological agent EgFB with different adjuvants for 120 days. The adjuvant substances ensuring as components of the biological agent EgFB a long circulation of specific antibodies to the Echinococcus antigens in the blood flow of the immunized animals (complete Freund's adjuvant, multicomponent oil emulsion) are presented.
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D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000918:Antibody Specificity; D000947:Antigens, Helminth / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004443:Echinococcosis / Q000097:blood*; D048209:Echinococcus granulosus / Q000276:immunology*; D004655:Emulsions / Q000494:pharmacology; D005620:Freund's Adjuvant / Q000494:pharmacology; D007114:Immunization; D007279:Injections, Subcutaneous; D009842:Oligopeptides / Q000494:pharmacology; D016052:Protozoan Vaccines / Q000008:administration & dosage / Q000276:immunology*; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology; D013997:Time Factors; D017160:Trichinella spiralis / Q000276:immunology* / Q000378:metabolism; D014235:Trichinellosis / Q000097:blood*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000187:drug effects / Q000276:immunology
D000276:Adjuvants, Immunologic; D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D004655:Emulsions; D009842:Oligopeptides; D016052:Protozoan Vaccines; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D005620:Freund's Adjuvant