title: Potencies of phosphine peptide inhibitors of mammalian thimet oligopeptidase and neurolysin on two bacterial pz peptidases.
abstract: Pz peptidases A and B, from a thermophile Geobacillus collagenovorans MO-1, recognize collagen-specific tripeptide units (Gly-Pro-Xaa). They share similarities in function but extremely low identities in primary sequence with mammalian thimet oligopeptidase (TOP) and neurolysin. Three phosphine peptide inhibitors that selectively inhibit TOP and neurolysin on two bacterial Pz peptidases were investigated. They showed potent inhibition of both Pz peptidases in a range from 10 to 100 nM.
authors.affiliation: Department of Applied Biochemistry, Kyoto Prefectural University, Sakyo, Kyoto, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Sugihara
authors.forename: Akio
authors.initials: A
authors.lastname: Kawasaki
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Tsujimoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Matsui
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D007700:Kinetics; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000378:metabolism*; D008970:Molecular Weight; D010720:Phosphines / Q000494:pharmacology*; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D011480:Protease Inhibitors / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D010720:Phosphines; D011480:Protease Inhibitors; D011994:Recombinant Proteins; D008666:Metalloendopeptidases; C022510:thimet oligopeptidase; C062316:neurolysin