Identifying new human oocyte marker genes: a microarray approach.
The efficacy of classical IVF techniques is still impaired by poor implantation and pregnancy rates after embryo transfer. This is mainly due to a lack of reliable criteria for the selection of embryos with sufficient development potential. Several studies have provided evidence that some gene expression levels could be used as objective markers of oocyte and embryo competence and capacity to sustain a successful pregnancy. These analyses usually use reverse transcription-polymerase chain reaction to look at small sets of pre-selected genes. However, microarray approaches allow the identification of a wider range of cellular marker genes which could include additional and perhaps more suitable genes that could serve as embryo selection markers. Microarray screenings of around 30,000 genes on U133P Affymetrix gene chips made it possible to establish the expression profile of these genes as well as other related genes in human oocytes and cumulus cells. This study identifies new potential regulators and marker genes such as BARD1, RBL2, RBBP7, BUB3 or BUB1B, which are involved in oocyte maturation.
Gasca
Stéphan
S
CHU Montpellier, Institut de Recherche en Biothérapie, Hôpital Saint-Eloi, Montpellier, F-34295, France.
Pellestor
Franck
F
Assou
Saïd
S
Loup
Vanessa
V
Anahory
Tal
T
Dechaud
Hervé
H
De Vos
John
J
Hamamah
Samir
S
D025601:Adenomatous Polyposis Coli Protein / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism; D010064:Embryo Implantation / Q000502:physiology; D020869:Gene Expression Profiling; D005819:Genetic Markers; D006801:Humans; D064246:Mad2 Proteins; D020411:Oligonucleotide Array Sequence Analysis; D009865:Oocytes / Q000254:growth & development / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases; D012097:Repressor Proteins / Q000378:metabolism; D016160:Retinoblastoma Protein / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000378:metabolism
D025601:Adenomatous Polyposis Coli Protein; D002135:Calcium-Binding Proteins; D018797:Cell Cycle Proteins; D005819:Genetic Markers; C577697:MAD2L1 protein, human; D064246:Mad2 Proteins; D012097:Repressor Proteins; D016160:Retinoblastoma Protein; D025521:Tumor Suppressor Proteins; D011494:Protein Kinases; C090464:Bub1 spindle checkpoint protein; D017346:Protein Serine-Threonine Kinases