Inhibition of avian metapneumovirus (AMPV) replication by RNA interference targeting nucleoprotein gene (N) in cultured cells.
Avian metapneumovirus (AMPV) is the primary causative agent of severe rhinotracheitis in turkeys. It is associated with swollen head syndrome in chickens and is the source of significant economic losses to animal food production. In this study, we designed specific short interfering RNA (siRNA) targeting the AMPV nucleoprotein (N) and fusion (F) genes. Three days post-virus infection, virus titration, real time RT-PCR, and RT-PCR assays were performed to verify the effect of siRNA in AMPV replication. A marked decrease in virus titers from transfected CER cells treated with siRNA/N was observed. Also, the production of N, F, and G mRNAs in AMPV was decreased. Results indicate that N-specific siRNA can inhibit virus replication. In future studies, a combination of siRNAs targeting the RNA polymerase complex may be used as a tool to study AMPV replication and/or antiviral therapy.
Ferreira
Helena Lage
HL
Laboratório de Virologia Animal, Departamento de Microbiologia e Imunologia, IB/UNICAMP, PO Box 6109, Campinas CEP: 13083970, SP, Brazil. arns@unicamp.br
Spilki
Fernando Rosado
FR
de Almeida
Renata Servan
RS
Santos
Márcia M A B
MM
Arns
Clarice Weis
CW
D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D029121:Metapneumovirus / Q000235:genetics / Q000254:growth & development*; D018184:Paramyxoviridae Infections / Q000821:virology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000138:chemical synthesis / Q000235:genetics; D012367:RNA, Viral / Q000138:chemical synthesis / Q000235:genetics; D013045:Species Specificity; D014162:Transfection / Q000379:methods; D014760:Viral Fusion Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics; D014779:Virus Replication
D034741:RNA, Small Interfering; D012367:RNA, Viral; D014760:Viral Fusion Proteins; D014764:Viral Proteins