title: Hybrid polymer/thin film impedance system for label free monitoring of cells.
abstract: Impedance measurements (capacitive cytometry) have been used to perform label-free analysis of cells. Two devices were constructed using a simple liquid photopolymerization technique known as muFluidicTectonics. This platform has made it possible to rapidly fabricate with great ease and without the use of traditional MEMS technology a cell impedance measurement system. Measurements were made using not only a single 1 kHz frequency but also a frequency sweep from 50 Hz to 20 kHz. Single frequency measurements on SP2 mouse cells showed show that there is a strong, linear relationship between the DNA content of individual cells and their dielectric (or capacitance) response to a 1 kHz field. Frequency sweeps were conducted on SF9 cells as means to perform diagnostic measurements of the device when different number of cells are present.
authors.affiliation: Dept. of Biomed. Eng., Wisconsin Univ., Madison, WI, USA.
authors.forename: Swomitra K
authors.initials: SK
authors.lastname: Mohanty
authors.forename: Lydia L
authors.initials: LL
authors.lastname: Sohn
authors.forename: David J
authors.initials: DJ
authors.lastname: Beebe