On-line monitoring of changes in host cell physiology during the one-step growth cycle of Bacillus phage Bam35.
In this study an on-line electrochemical method was developed to examine the one-step growth cycle (OSGC) of the bacteriophage Bam35. The on-line conditions for monitoring the OSGC and the effect of aeration on the duration of the OSGC were defined. The data indicate that binding of phenyldicarbaundecaborane anions to Bacillus thuringiensis cells infected with Bam35 can be used as a sensitive indicator of cell lysis.
Daugelavicius
Rimantas
R
Department of Biological and Environmental Sciences and Institute of Biotechnology, University of Helsinki, Finland.
Gaidelyte
Ausra
A
Cvirkaite-Krupovic
Virginija
V
Bamford
Dennis H
DH
D017103:Bacillus Phages / Q000254:growth & development*; D001413:Bacillus thuringiensis / Q000378:metabolism / Q000821:virology*; D001896:Boron Compounds / Q000378:metabolism; D018929:Cell Culture Techniques; D008242:Lysogeny / Q000502:physiology*; D013997:Time Factors
D001896:Boron Compounds; C100079:phenyldicarbaundecaborane