title: [Multi-dimensional fingerprints of Japan Jiuxin pill].
abstract: OBJECTIVE
  To develop HPLC-UV/ELSD and HPLC-UV/MS3 fingerprints of Japan Jiuxin pill using multi-dimensional hyphenated methods and study on its chemical base.
  
  
  METHODS
  Separation was performed on Altima C18 analytical column (250 mm x 4.6 mm ID, 5 microm). The mobile phases consisted of water containing 0.2% formic acid and acetonitrile was used as gradient elute. The flow rate was 1.0 mL x min(-1). An electrospray ion trap mass spectrometer was utilized for qualitative analysis and both positive and negative secondary ion scan mode were applied.
  
  
  RESULTS
  HPLC-UV/ELSD and HPLC-UV/ MS3 fingerprints of Japan Jiuxin pill with well separation and reproducibility were obtained. Totally 21 components in the complex formula were identified and the chemical structural information of glycocholic acid and its isomer were suggested.
  
  
  CONCLUSIONS
  The research is propitious to offer an effective and reliable pattern for analyzing Japanese Kampo Medicines as well as its quality control.
authors.affiliation: Jiangxi College of Traditional Chinese Medicine, Nanchang 330006, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Wang
authors.forename: Guo-An
authors.initials: GA
authors.lastname: Luo
mesh_terms: D002317:Cardiovascular Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D006331:Heart Diseases / Q000188:drug therapy*; D006801:Humans; D007564:Japan; D008516:Medicine, Chinese Traditional; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D002317:Cardiovascular Agents