Novel elastic material from collagen for tissue engineering.
Elastic collagen gel (e-gel) was prepared from salmon atelocollagen fibrillar gel reinforced by 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) mediated cross-linking (f-gel). The preparation consisted of a simple heat treatment of the f-gel at 80 degrees C, in which the f-gel drastically shrank and the collagen fibril structure was deformed. The e-gel obtained showed rubber-like elasticity; its stress-strain behavior little changed through repeated stretching. The elongation at the breaking point was approximately 230%. Furthermore, normal human osteoblasts showed good attachment and proliferation on the e-gel. These results suggest its potential to be utilized for the development of tissue engineering.
Yunoki
Shunji
S
Division of Molecular Chemistry, Graduate School of Engineering, Hokkaido University, Kita-13, Nishi-8, Kita-ku, Sapporo, Hokkaido 060-8628, Japan. yunoki@cris.hokudai.ac.jp
Mori
Kazuo
K
Suzuki
Takeshi
T
Nagai
Nobuhiro
N
Munekata
Masanobu
M
D001672:Biocompatible Materials / Q000737:chemistry*; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D004548:Elasticity; D024022:Fibrillar Collagens / Q000737:chemistry* / Q000648:ultrastructure*; D006244:Hardness; D006801:Humans; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D013314:Stress, Mechanical; D013718:Tensile Strength; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D024022:Fibrillar Collagens