Increased plasma malondialdehyde and fructosamine in anemic H pylori infected patients: effect of treatment.
OBJECTIVE
To unravel the possible association of malondialdehyde (MDA) and fructosamine in anemic H pylori infected patients and to observe the alteration in MDA and fructosamine levels in these patients after treatment for one month.


METHODS
Fructosamine, MDA and glucose were estimated in 22 anemic H pylori infected patients and 16 healthy controls. Hematological parameters were also evaluated in both the groups using Sysmex-K-100 automated cell counter. The H pylori infected patients were randomly divided into two groups. H pylori infected patients in Group I received both iron supplementation and anti-H pylori therapy, while patients in Group II received only iron supplementation. All the biochemical and hematological parameters were estimated after one month of treatment.


RESULTS
In anemic H pylori infected patients, while MDA (5.41 +/- 2.16 vs 2.26 +/- 0.50; P < 0.05) and fructosamine (2.64 +/- 0.93 vs 1.60 +/- 0.35; P < 0.05) were significantly increased, iron (32.72 +/- 14.93 vs 110.25 +/- 26.58; P < 0.05), hemoglobin (6.9 +/- 2.6 vs 12.66 +/- 0.74; P < 0.05) and ferritin (28.82 +/- 16.27 vs 140.43 +/- 30.72; P < 0.05) levels were significantly decreased compared with the controls. With partial correlation analysis, fructosamine was found to have a significant positive correlation with MDA. In Group I, while MDA level decreased significantly (3.11 +/- 1.73 vs 5.50 +/- 2.46; P < 0.05), there was a significant increase in iron (84.09 +/- 29.51 vs 36.09 +/- 17.81; P < 0.05), hemoglobin (10.40 +/- 1.11 vs 7.42 +/- 1.90; P < 0.05) and ferritin (116.91 +/- 63.34 vs 30.46 +/- 17.81; P < 0.05) levels after one month. There was no significant change in the levels of fructosamine in group I after treatment. Similarly, no significant alterations were noted in the levels of MDA, fructosamine, hemoglobin or ferritin in Group II patients after one month of treatment.


CONCLUSIONS
An increased level of fructosamine and MDA was found in anemic H pylori infected patients. Present data supports the premise that lipid peroxides per se do play a role in the glycation of plasma proteins. Furthermore, the findings from this study indicate that treatment for both anemia and H pylori infections is required for lowering the levels of lipid peroxides in these patients.
Vijayan
G
G
Department of Biochemistry, Jawaharlal Institute of Postgraduate Medical Education and Research, Pondicherry 605006, India.
Sundaram
R C
RC
Bobby
Zachariah
Z
Hamide
Abdoul
A
Selvaraj
N
N
Dasse
N Rattina
NR
D000740:Anemia / Q000188:drug therapy* / Q000378:metabolism* / Q000382:microbiology; D000900:Anti-Bacterial Agents / Q000008:administration & dosage; D019270:Fructosamine / Q000097:blood*; D016481:Helicobacter Infections / Q000150:complications / Q000188:drug therapy / Q000378:metabolism*; D016480:Helicobacter pylori; D006801:Humans; D007501:Iron / Q000008:administration & dosage*; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000097:blood*
D000900:Anti-Bacterial Agents; D019270:Fructosamine; D008315:Malondialdehyde; D007501:Iron