title: Variations of agar screen tests for detection of methicillin resistance in staphylococci: focus on cefoxitin.
abstract: Members of the genus Staphylococcus are among the most important human pathogens, and strains demonstrating resistance to methicillin are an increasing problem worldwide, both within and outside of hospital environments. The objective of this study was to evaluate the use of variations of agar screening tests with cefoxitin and oxacillin to detect methicillin resistance in staphylococcal isolates. The agar screening test with cefoxitin (4 microg/ml) showed 99.4% accuracy for detecting both S. aureus and coagulase-negative staphylococci. The performance of the agar screening test with cefoxitin (4 microg/ml) either equaled or was superior to the other agar screening test variations evaluated and can be used to characterize the presence of the mecA gene among staphylococcal species.
authors.affiliation: Microbiology Department, Fundação Faculdade Federal de Ciências Médicas de Porto Alegre, Sarmento Leite Street 245/211, 90050-170 Porto Alegre, RS, Brazil. leandro.reus@gmail.com
authors.forename: L R R
authors.initials: LR
authors.lastname: Perez
authors.forename: A L S
authors.initials: AL
authors.lastname: Antunes
authors.forename: A L
authors.initials: AL
authors.lastname: Barth
authors.forename: P A
authors.initials: PA
authors.lastname: d'Azevedo
mesh_terms: D000362:Agar; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D002440:Cefoxitin / Q000494:pharmacology*; D006801:Humans; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests / Q000379:methods*; D010068:Oxacillin / Q000494:pharmacology; D046915:Penicillin-Binding Proteins; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000382:microbiology; D013210:Staphylococcus / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D046915:Penicillin-Binding Proteins; C487789:mecA protein, Staphylococcus aureus; D002440:Cefoxitin; D000362:Agar; D010068:Oxacillin