title: Functional genomics of calcium channels in human melanoma cells.
abstract: Ca(2+)-signaling of human melanoma is in the focus of intensive research since the identification of the role of WNT-signaling in melanomagenesis. Genomic and functional studies pointed to the important role of various Ca(2+) channels in melanoma, but these data were contradictory. In the present study we clearly demonstrate, in a number of different ways including microarray analysis, DNA sequencing and immunocytochemistry, that various human melanoma cell lines and melanoma tissues overexpress ryanodine receptor type 2 (RyR2) and express P2X(7) channel proteins as compared to melanocytes. These channels, although retain some of their usual characteristics and pharmacological properties, display unique features in melanoma cells, including a functional interaction between the two molecules. Unlike P2X(7), RyR2 does not function as a calcium channel. On the other hand, the P2X(7) receptor has an antiapoptotic function in melanoma cells, since ATP-activation suppresses induced apoptosis, while knock down of the gene expression significantly enhances that.
authors.affiliation: Department of Physiology, Research Center for Molecular Medicine, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary.
authors.forename: Tamás
authors.initials: T
authors.lastname: Deli
authors.forename: Norbert
authors.initials: N
authors.lastname: Varga
authors.forename: Attila
authors.initials: A
authors.lastname: Adám
authors.forename: István
authors.initials: I
authors.lastname: Kenessey
authors.forename: Erzsébet
authors.initials: E
authors.lastname: Rásó
authors.forename: László G
authors.initials: LG
authors.lastname: Puskás
authors.forename: József
authors.initials: J
authors.lastname: Tóvári
authors.forename: János
authors.initials: J
authors.lastname: Fodor
authors.forename: Mónika
authors.initials: M
authors.lastname: Fehér
authors.forename: Gyula P
authors.initials: GP
authors.lastname: Szigeti
authors.forename: László
authors.initials: L
authors.lastname: Csernoch
authors.forename: József
authors.initials: J
authors.lastname: Tímár
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D015972:Gene Expression Regulation, Neoplastic; D015894:Genome, Human / Q000235:genetics*; D006801:Humans; D008544:Melanocytes / Q000187:drug effects / Q000378:metabolism; D008545:Melanoma / Q000235:genetics* / Q000378:metabolism*; D009506:Nevus / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D018048:Receptors, Purinergic P2 / Q000235:genetics / Q000378:metabolism; D058486:Receptors, Purinergic P2X7; D019837:Ryanodine Receptor Calcium Release Channel / Q000235:genetics* / Q000378:metabolism*
chemical_list: C546468:P2RX7 protein, human; D034741:RNA, Small Interfering; D018048:Receptors, Purinergic P2; D058486:Receptors, Purinergic P2X7; D019837:Ryanodine Receptor Calcium Release Channel; D000255:Adenosine Triphosphate; D002118:Calcium