title: Haplotype frequencies of 16 Y-chromosome STR loci in the Barcelona metropolitan area population using Y-Filer kit.
abstract: Haplotype frequencies for 16 Y-chromosomal short tandem repeat (STR) loci, included in the Y-Filer kit, were determined in 247 unrelated healthy individuals from the Barcelona metropolitan area (Catalonia, NE Spain). After PCR amplification and denaturing PAGE electrophoresis, DYS456, DYS389I, DYS390, DYS389II, DYS458, DYS19, DYS385a/b, DYS393, DYS391, DYS439, DYS635, DYS392, Y GATA H4.1, DYS437, DYS438 and DYS448 loci were typed. The aim of this study is to evaluate the performance in our population of the 16 loci of the Y-chromosome present in the new Y-Filer commercial identification kit, and acquire haplotype frequencies for mathematic processing of the forensic diagnosis in our geographical working area. In this sample, all haplotypes were unique. From the forensic point of view, the combined polymorphisms of the Y-Filer kit provide a high diagnostic efficiency.
authors.affiliation: Legal Medicine Department, Faculty of Medicine, University of Barcelona, Barcelona, Catalonia, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Sánchez
authors.forename: C
authors.initials: C
authors.lastname: Barrot
authors.forename: A
authors.initials: A
authors.lastname: Xifró
authors.forename: M
authors.initials: M
authors.lastname: Ortega
authors.forename: I Gómez
authors.initials: IG
authors.lastname: de Aranda
authors.forename: E
authors.initials: E
authors.lastname: Huguet
authors.forename: J
authors.initials: J
authors.lastname: Corbella
authors.forename: M
authors.initials: M
authors.lastname: Gené
mesh_terms: D016172:DNA Fingerprinting / Q000379:methods*; D030561:Databases, Nucleic Acid; D005787:Gene Frequency; D050173:Genes, Y-Linked / Q000235:genetics*; D014644:Genetic Variation; D006239:Haplotypes; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D013030:Spain; D014505:Urban Population