Autonomic neurotransmitters modulate immunoglobulin A secretion in porcine colonic mucosa.
Secretory immunoglobulin A (sIgA) plays a crucial role in mucosal surface defense. We tested the hypothesis that colonic sIgA secretion is under enteric neural control. Immunohistochemistry of the porcine distal colonic mucosa revealed presumptive cholinergic and adrenergic nerve fibers apposed to secretory component (SC)-positive crypt epithelial cells and neighboring IgA(+) plasmacytes. The cholinomimetic drug carbamylcholine elicited rapid, atropine-sensitive IgA secretion into the luminal fluid bathing mucosal explants mounted in Ussing chambers. The adrenergic receptor agonist norepinephrine also increased IgA secretion, an action inhibited by phentolamine. These effects were independent of agonist-induced anion secretion. In Western blots of luminal fluid, both agonists increased the density of protein bands co-immunoreactive for IgA and SC. Mucosal exposure to enterohemorrhagic Escherichia coli did not affect IgA secretion, and carbamylcholine treatment did not affect mucosal adherence of this enteropathogen. Acetylcholine and norepinephrine, acting respectively through muscarinic cholinergic and alpha-adrenergic receptors in the colonic mucosa, stimulate sIgA secretion and may enhance mucosal defense in vivo.
Schmidt
Lisa D
LD
Department of Veterinary and Biomedical Sciences, University of Minnesota, 1988 Fitch Avenue, St. Paul, Minnesota 55108-6010, USA.
Xie
Yonghong
Y
Lyte
Mark
M
Vulchanova
Lucy
L
Brown
David R
DR
D000818:Animals; D015153:Blotting, Western; D002217:Carbachol / Q000494:pharmacology; D018679:Cholinergic Agonists / Q000494:pharmacology; D017615:Enteric Nervous System / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology; D005260:Female; D018928:Immunity, Mucosal; D007070:Immunoglobulin A / Q000096:biosynthesis* / Q000187:drug effects; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000276:immunology* / Q000294:innervation* / Q000378:metabolism; D008297:Male; D018377:Neurotransmitter Agents / Q000378:metabolism*; D009638:Norepinephrine / Q000378:metabolism; D012635:Secretory Component / Q000378:metabolism; D013552:Swine
D018679:Cholinergic Agonists; D007070:Immunoglobulin A; D018377:Neurotransmitter Agents; D012635:Secretory Component; D002217:Carbachol; D009638:Norepinephrine