Measurements of oxidative stress status and antioxidant activity in chronic leukaemia patients.
There is an interactive relationship between leukaemia and oxidative stress. Leukaemic cells produce larger amounts of reactive oxygen species (ROS) than non-leukaemic cells as they are under a continual state of oxidative siege. So, this study was performed on 20 patients with chronic leukaemia from the Oncology Centre, Mansoura University. We measured leucocytic H(2)O(2) concentrations and lipid peroxidation as serum malondialdehyde (MDA) concentration, serum total antioxidant activity, plasma ascorbic acid and dehydroascorbic acid concentrations, blood reduced glutathione concentration, haemolysate G6PD activity, blood catalase activity, serum superoxide dismutase (SOD) activity and serum anti-dsDNA concentration. We found that chronic leukaemia patients showed a significant increase (P < 0.05) in leucocytic H(2)O(2), serum MDA concentration and total antioxidant activity either before or after treatment as compared with control group. Also, there was a significant increase in the other parameters (glutathione, catalase and SOD) either before or after treatment, but we found a significant decrease in ascorbic acid concentration and G6PD activity. There was a significant increase in anti-dsDNA concentration either before or after treatment. It can be concluded that leukaemic patients produce larger amounts of ROS than non-leukaemic patients. Also, the increase in antioxidant activity in leukaemic patients is not high enough to counteract the harmful effects of free radicals. This scenario becomes worse after administration of chemotherapy.
Al-Gayyar
Mohammed M H
MM
Dept. of Biochemistry, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.
Eissa
Laila A
LA
Rabie
Ahmed M
AM
El-Gayar
Amal M
AM
D000293:Adolescent; D000328:Adult; D000368:Aged; D000974:Antibodies, Antinuclear; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D000975:Antioxidants / Q000378:metabolism*; D002908:Chronic Disease; D005260:Female; D005609:Free Radicals; D006801:Humans; D007938:Leukemia / Q000188:drug therapy* / Q000503:physiopathology*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism
D000974:Antibodies, Antinuclear; D000970:Antineoplastic Agents; D000975:Antioxidants; D005609:Free Radicals; D017382:Reactive Oxygen Species