[Assessment of angiogenesis in children' s osteosarcoma].
BACKGROUND
Tumour angiogenesis is one of the most important hallmarks of cancer, which enables its development, progression and metastasizing. The extent of angiogenesis seems to be an essential prognostic factor in many solid tumours of children and adults. There have also been reports on the significance of angiogenesis in osteosarcoma. The most common methods to estimate angiogenesis is assessment of microvessel density (MVD) and vascular endothelial growth factor (VEGF) expression.


OBJECTIVE
The aim of the study was assessment of angiogenesis in osteosarcoma patients treated in our centre on the basis of MVD and VEGF expression.


METHODS
Histopathological specimens of 16 patients with osteosarcoma aged 9-18 years (median 13.5), treated in Department of Paediatrics, Paediatric Gastroenterology and Paediatric Oncology of Medical University in Gdansk, between 1997-2004, were studied retrospectively. Immunochemistry was performed using anti CD34 monoclonal antibody and chromogen to highlight vessels, which were counted at 200 x magnification on 3 microscopic fields. In the same specimens VEGF expression was evaluated semiquantitatively using immunohistochemical method. Patients were divided into two groups depending on presence of metastases. The two parameters were also compared in patients who died and the survivors.


RESULTS
11 of 16 patients are alive, with time of follow up 19-100 months (median 52). Five children died. Mean vascular density ranged from 25 to 87 (46 +/- 16.5). No significant statistical difference in microvessel density between metastatic and non-metastatic patients was observed. Microvessel density in these groups is 46.8 +/- 22.7 and 45.2 +/-7.0 respectively. In the group of survivors MVD was 44.3 +/- 5.9, inpatients who died it was 47.1 +/- 21.0 showing no significant statistical difference. In all patients positive VEGF expression was seen. Only one patient presented low expression of VEGF, the rest had high or medium degree of VEGF expression. MVD in the group with high expression of VEGF was higher than in the group with low and medium expression of VEGF. It was 51.8 +/- 18.7 and 39.2 +/- 14.2. The difference was not significant.


CONCLUSIONS
In the presented group of patients no differences in the extent of angiogenesis were seen in relation to treatment outcome or presence of metastases.
Sznurkowska
Katarzyna
K
Klinika Pediatrii, Gastroenterologii i Onkologii Dzieciecej, Akademia Medyczna, ul. Nowe Ogrody 1-6, 80-803 Gdańsk, Poland. k.sznurkowska@amg.gda.pl
Lenckowski
Radosław
R
Popadiuk
Stefan
S
Szumera
Małgorzata
M
Renke
Joanna
J
Korzon
Maria
M
D000293:Adolescent; D000911:Antibodies, Monoclonal; D018952:Antigens, CD34 / Q000032:analysis; D001859:Bone Neoplasms / Q000098:blood supply* / Q000378:metabolism / Q000628:therapy; D002648:Child; D005260:Female; D006801:Humans; D007120:Immunochemistry; D008297:Male; D009389:Neovascularization, Pathologic / Q000175:diagnosis* / Q000378:metabolism*; D012516:Osteosarcoma / Q000098:blood supply* / Q000378:metabolism / Q000628:therapy; D011044:Poland; D012189:Retrospective Studies; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D018952:Antigens, CD34; D042461:Vascular Endothelial Growth Factor A