title: Metabolic analysis of the cinnamate/monolignol pathway in Carthamus tinctorius seeds by a stable-isotope-dilution method.
abstract: The present study established a system for comprehensive metabolic analysis of the cinnamate/monolignol and lignan pathways by the use of a stable-isotope-dilution method. The system was successfully applied to characterization of the pathways in Carthamus tinctorius cv. Round-leaved White maturing seeds in combination with administration of stable-isotope-labelled precursors. Experimental results obtained using this technique strongly suggested the intermediacy of ferulic acid in lignan biosynthesis in the plant.
authors.affiliation: Research Institute for Sustainable Humanosphare, Kyoto University, Uji, Kyoto 611-0011, Japan.
authors.forename: Norikazu
authors.initials: N
authors.lastname: Sakakibara
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Nakatsubo
authors.forename: Shiro
authors.initials: S
authors.lastname: Suzuki
authors.forename: Daisuke
authors.initials: D
authors.lastname: Shibata
authors.forename: Mikio
authors.initials: M
authors.lastname: Shimada
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Umezawa
mesh_terms: D002247:Carbon Isotopes; D031187:Carthamus tinctorius / Q000378:metabolism*; D002934:Cinnamates / Q000737:chemistry / Q000378:metabolism*; D003903:Deuterium; D008401:Gas Chromatography-Mass Spectrometry; D007201:Indicator Dilution Techniques; D017705:Lignans / Q000096:biosynthesis* / Q000737:chemistry; D008031:Lignin / Q000096:biosynthesis* / Q000737:chemistry; D015394:Molecular Structure; D012639:Seeds / Q000378:metabolism
chemical_list: D002247:Carbon Isotopes; D002934:Cinnamates; D017705:Lignans; D008031:Lignin; D003903:Deuterium