Novel quasi-interpenetrating network/gold nanoparticles composite matrices for DNA sequencing by CE.
In order to further improve ssDNA sequencing performances using quasi-interpenetrating network (quasi-IPN) as a matrix composed of linear polyacrylamide (LPA) with lower viscosity-average molecular mass (3.3 MDa) and poly(N,N-dimethylacrylamide) (PDMA), gold nanoparticles (GNPs) were prepared and added into this quasi-IPN to form polymer/metal composite sieving matrices. The studies of intrinsic viscosity and differential scanning calorimetry (DSC) on quasi-IPN and quasi-IPN/GNPs indicate that there were interactions between GNPs and polymer chains. The sequencing performances on ssDNA using quasi-IPN and quasi-IPN/GNPs (with different GNPs concentrations) as sieving matrices were studied and compared by CE at different temperatures. The results show that resolutions of quasi-IPN/GNPs were higher than those of quasi-IPN without GNPs and approximated those of quasi-IPN composed of LPA with higher MW (6.5 MDa) and PDMA without GNPs in the bare fused-silica capillaries. Furthermore, the sequencing time of quasi-IPN/GNPs was shorter than that of quasi-IPN under the same sequencing conditions. The influences of GNPs and sequencing temperature on the sequencing performances of ssDNA were also discussed. The separation reproducibility of quasi-IPN/GNPs solution was excellent and its shelf life was more than 8 months.
Zhou
Dan
D
Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, PR China.
Wang
Yanmei
Y
Zhang
Wenlong
W
Yang
Runmiao
R
Shi
Ronghua
R
D000178:Acrylamides / Q000737:chemistry; D000180:Acrylic Resins / Q000737:chemistry; D002152:Calorimetry, Differential Scanning; D004277:DNA, Single-Stranded / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D017422:Sequence Analysis, DNA / Q000379:methods*; D013696:Temperature; D014783:Viscosity; D000099166:Polyacrylamides
D000178:Acrylamides; D000180:Acrylic Resins; D004277:DNA, Single-Stranded; D006046:Gold; D000099166:Polyacrylamides; C429790:poly(N,N-dimethylacrylamide)