title: Construction of nanogold hollow balls with dendritic surface as immobilized affinity support for protein adsorption.
abstract: This contribution introduced the construction of nanosize gold hollow balls (NGB) with dendritic surface as the immobilized affinity support for aflatoxin B(1) antibody (anti-AFB(1)) adsorption, as a model protein, in biorecognition interface. The interaction between nanogold hollow balls and anti-AFB(1) was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, and quartz crystal microbalance (QCM) technique. The deposited mass of anti-AFB(1) on the nanogold hollow ball-modified electrodes was more than that on the nanogold particle-modified electrodes via QCM analysis. The cyclic voltammogram tends to be more irreversible with anti-AFB(1) concentration increased. The antigen-antibody interaction was examined by using the nanogold hollow ball-modified QCM probes. Experimental results show that the developed protein assay system is sensitive to the concentration of AFB(1) as low as 0.05 ng mL(-1). Thus, the nanogold hollow ball is a useful matrix, and can be used as the immobilized affinity support for the adsorption of other proteins.
authors.affiliation: College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China. liaoswu@msn.com
authors.forename: Jia Yao
authors.initials: JY
authors.lastname: Liao
mesh_terms: D000327:Adsorption; D016604:Aflatoxin B1 / Q000276:immunology; D000465:Algorithms; D000908:Antibodies, Fungal / Q000737:chemistry; D050091:Dendrimers; D004563:Electrochemistry; D006046:Gold / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000908:Antibodies, Fungal; D050091:Dendrimers; D011506:Proteins; D006046:Gold; D016604:Aflatoxin B1