title: Statistical evaluation of the isoform patterns of N-acetyl-beta-hexosaminidase from human renal cancer tissue separated by isoelectrofocusing.
abstract: OBJECTIVE
  Isoenzymes of HEX from human renal carcinoma and neighbouring macroscopically normal renal tissue can show different patterns on isoelectrofocusing gels. The aim of our work was to elaborate the method for statistical evaluation of differences.
  
  
  METHODS
  Isoenzymes of N-acetyl-beta-hexosaminidase were separated from renal (control and cancerous) tissues of 15 patients. Isoenzymes were electrofocused in Multiphor II, with ampholine pH 3.5-9.0 (2%) and then evaluated densitometrically and analysed statistically.
  
  
  RESULTS
  A similar pattern in activity of isoforms of isoenzymes A and B in normal and cancerous renal tissue was observed.
  
  
  CONCLUSIONS
  The proposed method of statistical evaluation of differences in isoforms of N-acetyl-beta-glucosaminidase can also be adapted to estimate the isoforms of other enzymes in different tissues.
authors.affiliation: Department of Pharmaceutical Biochemistry, Medical University, Mickiewicza 2A, 15-230 Białystok, Poland. gocha@amb.edu.pl
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Borzym-Kluczyk
authors.forename: Iwona
authors.initials: I
authors.lastname: Radziejewska
authors.forename: Ewa
authors.initials: E
authors.lastname: Olszewska
authors.forename: Sławomir
authors.initials: S
authors.lastname: Szajda
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Knaś
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Zwierz
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism; D003627:Data Interpretation, Statistical; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods*; D007527:Isoenzymes / Q000378:metabolism; D007680:Kidney Neoplasms / Q000201:enzymology*; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism*
chemical_list: D007527:Isoenzymes; D000118:Acetylglucosaminidase; D001619:beta-N-Acetylhexosaminidases