Multiparallel microfluidic high-performance liquid chromatography for high-throughput normal-phase chiral analysis.
The suitability of the Eksigent Express 800 microfluidic eight-channel HPLC instrument for multiparallel normal-phase chiral analysis in support of high-throughput pharmaceutical process research was investigated. Analysis of test mixtures containing the two enantiomers of benzoin and the closely related (R,S)-dihydrobenzoin, was carried out in a 96-well microplate, affording rapid (<2 h) and accurate assessment of enantiopurity. In a second example, use of the instrument to support high-throughput catalyst screening of the asymmetric hydrogenation of a prochiral unsaturated ester is presented, in which method development (gradient screening of four columns and two eluents, followed by optimization to afford a fast analytical method) and analysis of a 96-well microplate was carried out within a single working day. This represents a considerable improvement over conventional analysis techniques that usually take several days to complete.
Sajonz
Peter
P
Separation & Analysis Technologies, Merck and Co., Inc., Rahway, NJ 07065, USA. peter_sajonz@merck.com
Schafer
Wes
W
Gong
Xiaoyi
X
Shultz
Scott
S
Rosner
Thorsten
T
Welch
Christopher J
CJ
D001573:Benzoin / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006865:Hydrogenation; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D015394:Molecular Structure; D015203:Reproducibility of Results; D013237:Stereoisomerism
D001573:Benzoin