title: Polymorphism of the porcine leptin gene promoter and analysis of its association with gene expression and fatness traits.
abstract: We describe for the first time a 245 bp fragment of the porcine leptin gene promoter in the proximity of the transcription start site. Altogether, 720 pigs were screened with the PCR-SSCP technique for polymorphism in this region. Four SNPs, segregating as two haplotypes, have been identified, one of them (C113G) in the putative consensus site for the AP-2 transcription factor. This polymorphism was evenly distributed in the Duroc breed (n=21) and was absent in the Polish Landrace (n=248) and Pietrain breed (n=12). In the Polish Large White (n=191) and synthetic line 990 (n=243), allele G occurred with a very low frequency. The investigation was performed to test if the C113G SNP affects leptin mRNA level in subcutaneous fat and leptin protein concentration in serum. Additionally, the effect of this polymorphism on fatness traits was statistically analyzed. Although there was a trend toward decreased expression in GG animals, the differences were not significant between genotypes. We also found no evidence for an association of the LEP promoter genotype with the analyzed fatness traits.
authors.affiliation: Department of Genetics and Animal Breeding, Agricultural University of Poznan, Poznan, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Stachowiak
authors.forename: M
authors.initials: M
authors.lastname: Mackowski
authors.forename: Z
authors.initials: Z
authors.lastname: Madeja
authors.forename: M
authors.initials: M
authors.lastname: Szydlowski
authors.forename: A
authors.initials: A
authors.lastname: Buszka
authors.forename: P
authors.initials: P
authors.lastname: Kaczmarek
authors.forename: B
authors.initials: B
authors.lastname: Rubis
authors.forename: P
authors.initials: P
authors.lastname: Mackowiak
authors.forename: K W
authors.initials: KW
authors.lastname: Nowak
authors.forename: M
authors.initials: M
authors.lastname: Switonski
mesh_terms: D000273:Adipose Tissue / Q000033:anatomy & histology*; D000818:Animals; D001483:Base Sequence; D001835:Body Weight / Q000235:genetics; D005786:Gene Expression Regulation / Q000235:genetics*; D005787:Gene Frequency; D006239:Haplotypes; D020738:Leptin / Q000235:genetics*; D008969:Molecular Sequence Data; D010641:Phenotype; D011110:Polymorphism, Genetic / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D019655:Quantitative Trait, Heritable; D013552:Swine / Q000033:anatomy & histology / Q000235:genetics*
chemical_list: D020738:Leptin