[Expression of fms-like tyrosine kinase receptor 1 in placenta of pre-eclampsia].
OBJECTIVE
To investigate the expression of fms-like tyrosine kinase receptor 1 (Flt-1) in placentas of pre-eclampsia.


METHODS
The expression of Flt-1 mRNA in the placentas from 20 pre-eclampsia patients and 20 pregnant women with normal blood pressure was detected by semi-quantitative reverse transcription-polymerase chain reaction. The protein expression of Flt-1 was analyzed using western blot in 18 pre-eclampsia patients and 18 normotensive pregnant women.


RESULTS
Placental Flt-1 mRNA level in pre-eclampsia was 2.25 +/- 0.19 (intensity ratios of Flt-1 mRNA to beta-actin mRNA), significantly higher than in normotensive pregnant women 1.23 +/- 0.29 (P < 0.05). Western blot showed that Flt-1 protein level in pre-eclamptic placenta was 2.67 +/- 1.19 [western blot signal intensity ratios of Flt-1 to glyceraldehyde-3-phosphate dehydrogenase (GAPDH)], significantly higher than in pregnant women with normal blood pressure 0.94 +/- 0.51 (P < 0.05).


CONCLUSIONS
Increased Flt-1 expression in pre-eclamptic placenta may be involved in pathogenesis of pre-eclampsia.
Hou
Xiu-Hong
XH
Department of Obstetrics and Gynecology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
Li
Dong-Hong
DH
Li
Yi
Y
Zhao
Hong-Xi
HX
Zhang
Chun-Li
CL
Huang
Liang
L
Yao
Yuan-Qing
YQ
D000328:Adult; D015153:Blotting, Western; D005260:Female; D005865:Gestational Age; D006801:Humans; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000209:etiology / Q000378:metabolism*; D011247:Pregnancy; D011263:Pregnancy Trimester, Third; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000096:biosynthesis*
D012333:RNA, Messenger; C501162:FLT1 protein, human; D040281:Vascular Endothelial Growth Factor Receptor-1