Phylogenetic analysis of Heliothis armigera cytoplasmic polyhedrosis virus type 14 and a series of dwarf segments found in the genome.
Full-length nucleotide sequences for the genome segments (S1-S6) of Heliothis armigera cytoplasmic polyhedrosis virus type 14 (HaCPV-14) have been characterized. Each segment consists of a single open reading frame with conserved motifs AGAA and AGCU at the 5' and 3' ends, respectively. Comparison of the proteins of HaCPV-14 with those of other members of the family Reoviridae suggests that S1 encodes an RNA-dependent RNA polymerase (RdRp), whilst S2 encodes a major capsid protein of the virus. Phylogenetic analysis of RdRps from 16 viruses in the family Reoviridae reveals that the genera Cypovirus and Oryzavirus may have originated from a common insect virus ancestor. A series of viable dwarf segments originating from S5 of HaCPV-14 has been identified. Analysis of the predicted secondary structures for these dwarf segments suggests that the signals essential for replication and packaging are located within the terminal sequences of these segments.
Li
Yanqiu
Y
Laboratory of Insect Virology, State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, Hubei, China.
Zhang
Jiamin
J
Laboratory of Insect Virology, State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, Hubei, China.
Li
Yang
Y
Laboratory of Insect Virology, State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, Hubei, China.
Tan
Li
L
Laboratory of Insect Virology, State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, Hubei, China.
Chen
Wuguo
W
Laboratory of Insect Virology, State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, Hubei, China.
Luo
Haishan
H
Laboratory of Insect Virology, State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, Hubei, China.
Hu
Yuanyang
Y
Laboratory of Insect Virology, State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, Hubei, China.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics; D017124:Conserved Sequence / Q000235:genetics; D004261:DNA Replication; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009036:Moths / Q000821:virology; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D012087:Reoviridae / Q000145:classification* / Q000235:genetics*; D016415:Sequence Alignment; D017385:Sequence Homology; D014764:Viral Proteins / Q000235:genetics; D019065:Virus Assembly
D036022:Capsid Proteins; D004279:DNA, Viral; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase