title: Evaluation of the VITROS 5,1 FS chemistry system in a large pediatric laboratory.
abstract: The VITROS 5,1 FS analytical system was evaluated. The measurement of the serum proteins and HDL cholesterol with the newly developed MicroTip technology was compared with other nephelometric and turbidimetric methods. The within-run imprecision for apolipoprotein A1, apolipoprotein B, complement 3, complement 4, transferrin, immunoglobulin A, immunoglobulin M, immunoglobulin G and HDL cholesterol showed coefficients of variation between 0.4% and 4.7%. The day-to-day imprecision showed coefficients of variation between 0.5% and 4.0%. The correlation with the comparative methods (nephelometric or turbidimetric) was good, with Pearson correlation coefficients of between 0.959 and 0.995. The comparison of apolipoprotein Al showed a slope of about 0.76. An explanation for the difference could not be found. The VITROS 5,1 FS analytical system is a reliable routine instrument which fits into a large pediatric laboratory of a university.
authors.affiliation: Allgemeines Krankenhaus, Universitäts-Kinderklinik, Vienna, Austria.
authors.forename: Kurt
authors.initials: K
authors.lastname: Herkner
authors.forename: Rosmarie
authors.initials: R
authors.lastname: Reiter-Reisacher
authors.forename: Oswald
authors.initials: O
authors.lastname: Sonntag
mesh_terms: D001053:Apolipoproteins / Q000097:blood; D001774:Blood Chemical Analysis / Q000295:instrumentation* / Q000379:methods*; D008076:Cholesterol, HDL / Q000097:blood; D003165:Complement System Proteins / Q000032:analysis; D005069:Evaluation Studies as Topic; D006801:Humans; D007136:Immunoglobulins / Q000097:blood; D009391:Nephelometry and Turbidimetry / Q000295:instrumentation* / Q000379:methods*; D010372:Pediatrics / Q000295:instrumentation / Q000379:methods; D012044:Regression Analysis; D014168:Transferrin / Q000032:analysis
chemical_list: D001053:Apolipoproteins; D008076:Cholesterol, HDL; D007136:Immunoglobulins; D014168:Transferrin; D003165:Complement System Proteins