Mito-DEPMPO synthesized from a novel NH2-reactive DEPMPO spin trap: a new and improved trap for the detection of superoxide.
Mito-DEPMPO, a new DEPMPO analogue bearing a triphenylphosphonium group, was synthesized via a novel NH2-reactive DEPMPO. The half-life of the Mito-DEPMPO superoxide adduct was estimated to be ca. 40 min. Using Mito-DEPMPO, reactive oxygen species generated in intact mitochondria were detected and characterized by EPR.
Hardy
Micael
M
SREP, UMR 6517 CNRS et Universités Aix-Marseille 1, 2 et 3, Centre de Saint Jérôme, 13013 Marseille, France.
Chalier
Florence
F
Ouari
Olivier
O
Finet
Jean-Pierre
JP
Rockenbauer
Antal
A
Kalyanaraman
Balaraman
B
Tordo
Paul
P
D000818:Animals; D003497:Cyclic N-Oxides / Q000138:chemical synthesis* / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D019271:Hypoxanthine / Q000378:metabolism; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D010010:Osteoclasts / Q000166:cytology / Q000378:metabolism; D013113:Spin Labels; D019097:Spin Trapping; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*; D014969:Xanthine Oxidase / Q000378:metabolism
D003497:Cyclic N-Oxides; D013113:Spin Labels; D013481:Superoxides; C098505:5-diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide; D019271:Hypoxanthine; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase