Lysobacter niabensis sp. nov. and Lysobacter niastensis sp. nov., isolated from greenhouse soils in Korea.
Two bacterial strains, designated GH34-4(T) and GH41-7(T), were isolated from greenhouse soil cultivated with cucumber. The bacteria were strictly aerobic, Gram-negative, rod-shaped and oxidase- and catalase-positive. 16S rRNA gene sequence analysis indicated that these strains belong to the genus Lysobacter within the Gammaproteobacteria. Strain GH34-4(T) showed highest sequence similarity to Lysobacter yangpyeongensis GH19-3(T) (97.5 %) and Lysobacter koreensis Dae16(T) (96.4 %), and strain GH41-7(T) showed highest sequence similarity to Lysobacter antibioticus DSM 2044(T) (97.5 %), Lysobacter enzymogenes DSM 2043(T) (97.5 %) and Lysobacter gummosus ATCC 29489(T) (97.4 %). Levels of DNA-DNA relatedness indicated that strains GH34-4(T) and GH41-7(T) represented species clearly different from L. yangpyeongensis, L. antibioticus, L. enzymogenes and L. gummosus. The major cellular fatty acids of strains GH34-4(T) and GH41-7(T) were iso-C(16 : 0), iso-C(15 : 0) and iso-C(17 : 1)omega9c, and the major isoprenoid quinone was Q-8. The DNA G+C contents of GH34-4(T) and GH41-7(T) were 62.5 and 66.6 mol%, respectively. On the basis of the polyphasic taxonomic data presented, it is evident that each of these strains represents a novel species of the genus Lysobacter, for which the names Lysobacter niabensis sp. nov. (type strain GH34-4(T)=KACC 11587(T)=DSM 18244(T)) and Lysobacter niastensis sp. nov. (type strain GH41-7(T)=KACC 11588(T)=DSM 18481(T)) are proposed.
Weon
Hang-Yeon
HY
Applied Microbiology Division, National Institute of Agricultural Science and Technology, Rural Development Administration (RDA), Suwon 441-707, Republic of Korea.
Kim
Byung-Yong
BY
Korean Agricultural Culture Collection (KACC), Microbial Genetics Division, National Institute of Agricultural Biotechnology, RDA, Suwon 441-707, Republic of Korea.
Kim
Min-Kyeong
MK
Environment and Ecology Division, National Institute of Agricultural Science and Technology, RDA, Suwon 441-707, Republic of Korea.
Yoo
Seung-Hee
SH
Korean Agricultural Culture Collection (KACC), Microbial Genetics Division, National Institute of Agricultural Biotechnology, RDA, Suwon 441-707, Republic of Korea.
Kwon
Soon-Wo
SW
Korean Agricultural Culture Collection (KACC), Microbial Genetics Division, National Institute of Agricultural Biotechnology, RDA, Suwon 441-707, Republic of Korea.
Go
Seung-Joo
SJ
Korean Agricultural Culture Collection (KACC), Microbial Genetics Division, National Institute of Agricultural Biotechnology, RDA, Suwon 441-707, Republic of Korea.
Stackebrandt
Erko
E
Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, D-38124 Braunschweig, Germany.
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids / Q000032:analysis; D007723:Korea; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D012988:Soil Microbiology; D044166:Xanthomonadaceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S