title: [Autologous chondrocyte implantation in the treatment of cartilage lesions of ankle joint].
abstract: OBJECTIVE
  The authors present the results of surgical treatment of talar dome cartilage defects. They used the transplantation of autologous cultured chondrocytes in the form of a solid chondral graft.
  
  
  METHODS
  Patients with chondral lesions categorized as grades II to IV by the Anderson classification or as grades II to IV by the Berndt and Harty classification were indicated for this treatment. After preoperative MRI examination, a small sample of healthy cartilage was harvested arthroscopically from the non-weight-bearing area of the talus and was sent to the Tissue Bank in Brno for chondrocyte cultivation. After 28 to 42 days the cultured chondrocytes were formed into a solid chondral graft, implanted at the damaged site of the talar dome and fixed with fibrin glue (Tissucol).
  
  
  RESULTS
  Between July 2003 and October 2005 five patients, three males and two females, were treated using this method. Their age ranged from 22 to 46 years. Follow-up was 6 to 24 months, with an average of 12.6 months, and the patients were examined by MRI at 2 weeks, 2 and 6 months and at 1 year. The clinical results were evaluated on the basis of the Mazur and Weber scoring systems.
  
  
  CONCLUSIONS
  A significant improvement in clinical function of the ankle joint was achieved in three patients and the condition remained unchanged in one patient. In one patient, the surgical outcome was too recent for evaluation, but the MRI results indicated tendency to good incorporation of the graft.
authors.affiliation: Ortopedická klinika detí a dospelých 2. LF UK, FN Motol, Praha. milan.handl@lfmotol.cuni.cz
authors.forename: M
authors.initials: M
authors.lastname: Handl
authors.forename: T
authors.initials: T
authors.lastname: Trc
authors.forename: M
authors.initials: M
authors.lastname: Hanus
authors.forename: E
authors.initials: E
authors.lastname: Stastný
authors.forename: M
authors.initials: M
authors.lastname: Fricová-Poulová
authors.forename: J
authors.initials: J
authors.lastname: Neuwirth
authors.forename: J
authors.initials: J
authors.lastname: Adler
authors.forename: D
authors.initials: D
authors.lastname: Havranová
authors.forename: F
authors.initials: F
authors.lastname: Varga
mesh_terms: D000328:Adult; D016512:Ankle Injuries / Q000601:surgery*; D000843:Ankle Joint; D002358:Cartilage, Articular / Q000166:cytology / Q000293:injuries*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000637:transplantation*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D023822:Tissue Engineering