Redundant promoter elements mediate IL-3-induced expression of a novel cytokine-inducible gene, cyclon.
Cytokines control cell differentiation, proliferation, and function by regulating gene expression program. Physiological roles and induction mechanisms of cytokine-inducible genes are not fully understood. Here, we identified a novel immediate-early cytokine-responsive gene, cyclon (cytokine-induced protein with coiled-coil domain), which is induced in a hematopoietic cell line by interleukin 3 (IL-3). cyclon gene encodes a phosphorylated nuclear protein consisting of repetitive sequences in the amino-terminus and a coiled-coil domain in the carboxyl-terminus. A novel transient reporter assay revealed that mouse cyclon promoter contains redundant elements for IL-3-induced gene expression.
Hoshino
Akemi
A
Department of Pathology, New York University School of Medicine, 550 First Avenue, MSB-126, New York, NY 10016, USA.
Fujii
Hodaka
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology*; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D020411:Oligonucleotide Array Sequence Analysis; D010766:Phosphorylation; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017386:Sequence Homology, Amino Acid; D050826:Suppressor of Cytokine Signaling Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D017931:DNA Primers; D007377:Interleukin-3; D012333:RNA, Messenger; D050826:Suppressor of Cytokine Signaling Proteins