title: Characterization of the role of the receptors PEX5 and PEX7 in the import of proteins into glycosomes of Trypanosoma brucei.
abstract: Peroxins 5 and 7 are receptors for protein import into the peroxisomal matrix. We studied the involvement of these peroxins in the biogenesis of glycosomes in the protozoan parasite Trypanosoma brucei. Glycosomes are peroxisome-like organelles in which a major part of the glycolytic pathway is sequestered. We here report the characterization of the T. brucei homologue of PEX7 and provide several data strongly suggesting that it can bind to PEX5. Depletion of PEX5 or PEX7 by RNA interference had a severe effect on the growth of both the bloodstream-form of the parasite, that relies entirely on glycolysis for its ATP supply, and the procyclic form representative of the parasite living in the tsetse-fly midgut and in which also other metabolic pathways play a prominent role. The role of the two receptors in import of glycosomal matrix proteins with different types of peroxisome/glycosome-targeting signals (PTS) was analyzed by immunofluorescence and subcellular fractionation studies. Knocking down the expression of either receptor gene resulted, in procyclic cells, in the mislocalization of proteins with both a type 1 or 2 targeting motif (PTS1, PTS2) located at the C- and N-termini, respectively, and proteins with a sequence-internal signal (I-PTS) to the cytosol. Electron microscopy confirmed the apparent integrity of glycosomes in these procyclic cells. In bloodstream-form trypanosomes, PEX7 depletion seemed to affect only the subcellular distribution of PTS2-proteins. Western blot analysis suggested that, in both life-cycle stages of the trypanosome, the levels of both receptors are controlled in a coordinated fashion, by a mechanism that remains to be determined. The observation that both PEX5 and PEX7 are essential for the viability of the parasite indicates that the respective branches of the glycosome-import pathway in which each receptor acts might be interesting drug targets.
authors.affiliation: Research Unit for Tropical Diseases, Christian de Duve Institute of Cellular Pathology and Laboratory of Biochemistry, Université catholique de Louvain, Brussels, Belgium.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Galland
authors.forename: Fabian
authors.initials: F
authors.lastname: Demeure
authors.forename: Véronique
authors.initials: V
authors.lastname: Hannaert
authors.forename: Emilie
authors.initials: E
authors.lastname: Verplaetse
authors.forename: Didier
authors.initials: D
authors.lastname: Vertommen
authors.forename: Patrick
authors.initials: P
authors.lastname: Van der Smissen
authors.forename: Pierre J
authors.initials: PJ
authors.lastname: Courtoy
authors.forename: Paul A M
authors.initials: PA
authors.lastname: Michels
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D003001:Cloning, Molecular; D008018:Life Cycle Stages; D008830:Microbodies / Q000378:metabolism* / Q000648:ultrastructure; D008969:Molecular Sequence Data; D000074437:Peroxisomal Targeting Signal 2 Receptor; D000074435:Peroxisome-Targeting Signal 1 Receptor; D011485:Protein Binding; D021381:Protein Transport; D015800:Protozoan Proteins / Q000378:metabolism*; D034622:RNA Interference; D018160:Receptors, Cytoplasmic and Nuclear / Q000737:chemistry / Q000172:deficiency / Q000302:isolation & purification / Q000378:metabolism*; D020539:Sequence Analysis, Protein; D017385:Sequence Homology; D014346:Trypanosoma brucei brucei / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D000074437:Peroxisomal Targeting Signal 2 Receptor; D000074435:Peroxisome-Targeting Signal 1 Receptor; D015800:Protozoan Proteins; D018160:Receptors, Cytoplasmic and Nuclear