title: [Urethral reconstruction with buccal mucosa and colonic mucosa onlay grafts: experiment with dogs].
abstract: OBJECTIVE
  To investigate the possibility of urethral reconstruction with free buccal mucosa and colonic mucosa onlay graft.
  
  
  METHODS
  Twenty male mongrel dogs were randomly divided into 2 groups (n = 10 each). In group A: 4 cm x 0.8 cm segment of perineal urethra was excised and onlay urethroplasty was performed using free buccal mucosa. In group B: 4 cm x 0.8 cm segment of perineal urethra was excised and onlay urethroplasty was performed using free colonic mucosa. Retrograde urethrography was done at week 12. Autopsy specimens were calibrated with a 8Fr catheter. Graft length were measured immediately after scarification in all dogs. Histological study of all autopsy specimens was done.
  
  
  RESULTS
  Urethral structures were diagnosed by radiology and confirmed by calibration in 1 of the 10 dogs (10%) in group A and 2 of the 10 dogs (20%) in group B. Graft shrinkage was less than 20% for buccal mucosa and colonic mucosa. Histological examination revealed that the buccal mucosa and colonic mucosa survived inside the urethral lumen of all dogs. Plicae surface and unilaminar cylindric epithelium of the buccal mucosa and colonic mucosa were not observed and metaplastic transitional epithelium covered a large proportion of the urethral mucosa in all dogs.
  
  
  CONCLUSIONS
  Urethral mucosa can be replaced by colonic mucosa as well as buccal mucosa by using onlay urethroplasty.
authors.affiliation: Department of Urology, Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China.
authors.forename: Ying-long
authors.initials: YL
authors.lastname: Sa
authors.forename: Yue-min
authors.initials: YM
authors.lastname: Xu
authors.forename: Bin-feng
authors.initials: BF
authors.lastname: Chen
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.forename: Zhang-shun
authors.initials: ZS
authors.lastname: Liu
authors.forename: Chao
authors.initials: C
authors.lastname: Feng
mesh_terms: D000818:Animals; D004285:Dogs; D007413:Intestinal Mucosa / Q000637:transplantation*; D008297:Male; D023421:Models, Animal; D009061:Mouth Mucosa / Q000637:transplantation*; D011897:Random Allocation; D019651:Plastic Surgery Procedures / Q000379:methods; D013524:Surgical Flaps; D014521:Urethra / Q000601:surgery*; D013520:Urologic Surgical Procedures / Q000379:methods