Synthesis and evaluation of a novel lipid-peptide conjugate for functionalized liposome.
A novel lipid analog based on amino acids for liposome modification was developed. It consisted of three different kinds of amino acid derivatives and two fatty acids, and can react directly with the peptide synthesized first on resin by Fmoc solid-phase synthesis. In this study, lipid analog conjugated with HIV-TAT peptide (domain of human immunodeficiency virus TAT protein) was synthesized and successfully incorporated into liposome. The liposome containing the lipopeptide bearing HIV-TAT exhibited efficient cellular uptake.
Yagi
Nobuhiro
N
Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi, Chiba 274-8510, Japan.
Yano
Yuko
Y
Hatanaka
Kentaro
K
Yokoyama
Yuusaku
Y
Okuno
Hiroaki
H
D000818:Animals; D019556:COS Cells; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002522:Chlorocebus aethiops; D002851:Chromatography, High Pressure Liquid; D015195:Drug Design; D005227:Fatty Acids / Q000737:chemistry; D015696:Gene Products, tat / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D008968:Molecular Conformation; D010455:Peptides / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D005227:Fatty Acids; D015696:Gene Products, tat; D008055:Lipids; D008081:Liposomes; D010455:Peptides