PPE protein (Rv3425) from DNA segment RD11 of Mycobacterium tuberculosis: a potential B-cell antigen used for serological diagnosis to distinguish vaccinated controls from tuberculosis patients.
Proteins encoded by a 9.5-kb DNA segment, termed the region of difference (RD), of Mycobacterium tuberculosis have been demonstrated to be important in bacterial virulence, vaccine development and the design of diagnostic reagents. This study evaluated the immunogenic properties of Rv3425, a member of the PPE family of proteins, encoded by an open reading frame found in RD11 of M. tuberculosis, in comparison with two other well-known antigens, the early secreted antigen target 6 (ESAT-6) and the 10-kDa culture filtrate protein (CFP-10). RT-PCR demonstrated that Rv3425 mRNA is expressed in liquid culture by M. tuberculosis H37Rv. When tested in a conventional ELISA in the form of a His-tagged recombinant protein, Rv3425 revealed a statistically significant antigenic distinction between healthy bacille Calmette-Guérin (BCG)-vaccinated controls and tuberculosis (TB) patients (p <0.0001). The anti-IgG response to recombinant Rv3425 was almost equal to that for CFP-10, and was higher than that for ESAT-6. The results highlight the immunosensitive and immunospecific nature of Rv3425, which shows promise for use in the serodiagnosis of TB.
Zhang
H
H
State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai.
Wang
J
J
State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai.
Lei
J
J
State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai.
Zhang
M
M
State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai.
Yang
Y
Y
State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai.
Chen
Y
Y
No. 6 Hospital of Zhengzhou, Henan, China.
Wang
H
H
State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai. Electronic address: hhwang@fudan.edu.cn.
D000328:Adult; D000368:Aged; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D001500:BCG Vaccine / Q000276:immunology; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000276:immunology*; D016366:Open Reading Frames; D010446:Peptide Fragments / Q000276:immunology; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000276:immunology / Q000302:isolation & purification; D012698:Serologic Tests; D014376:Tuberculosis / Q000175:diagnosis / Q000276:immunology; D014611:Vaccination
D000942:Antigens, Bacterial; D001500:BCG Vaccine; D001426:Bacterial Proteins; D010446:Peptide Fragments; D012333:RNA, Messenger; D011994:Recombinant Proteins; C000592808:Rv3425 protein, Mycobacterium tuberculosis