In vivo fluorescent imaging of the mouse retina using adaptive optics.
In vivo imaging of the mouse retina using visible and near infrared wavelengths does not achieve diffraction-limited resolution due to wavefront aberrations induced by the eye. Considering the pupil size and axial dimension of the eye, it is expected that unaberrated imaging of the retina would have a transverse resolution of 2 microm. Higher-order aberrations in retinal imaging of human can be compensated for by using adaptive optics. We demonstrate an adaptive optics system for in vivo imaging of fluorescent structures in the retina of a mouse, using a microelectromechanical system membrane mirror and a Shack-Hartmann wavefront sensor that detects fluorescent wavefront.
Biss
David P
DP
Schepens Eye Research Institute, Boston, Massachusetts 02114, USA. david.biss@schepens.harvard.edu
Sumorok
Daniel
D
Burns
Stephen A
SA
Webb
Robert H
RH
Zhou
Yaopeng
Y
Bifano
Thomas G
TG
Côté
Daniel
D
Veilleux
Israel
I
Zamiri
Parisa
P
Lin
Charles P
CP
D000818:Animals; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005451:Fluorescein Angiography / Q000379:methods*; D007089:Image Enhancement / Q000295:instrumentation*; D007909:Lenses; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence / Q000379:methods*; D015203:Reproducibility of Results; D012160:Retina / Q000033:anatomy & histology*; D012680:Sensitivity and Specificity