title: Interleukin-24, RANTES and CCR5 gene polymorphisms are not associated with chronic adult periodontitis.
abstract: OBJECTIVE
  Cytokines, such as interleukin-10, and related genetic polymorphisms, have been implicated in the pathogenesis of chronic periodontitis. The aim of this study was to investigate a possible correlation between chronic periodontitis and genetic polymorphisms coding for two interleukin-10 related chemokines [interleukin-24 and regulated on activation, normal T cells expressed and secreted (RANTES)] as well as a RANTES receptor [CC chemokine receptor 5 (CCR5)].
  
  
  METHODS
  A single-blind, two-centre, case-controlled study was carried out with test patients from the Clinic of Periodontics, Göteborg University, and from the Department of Periodontology, Glasgow University, and control subjects from the undergraduate clinics of both schools. Blood samples were collected from 106 patients (56 women and 50 men, mean age 51.7 yr) with generalized, severe chronic periodontitis and from 69 periodontally healthy subjects (37 women and 32 men, mean age 53.3 yr). The polymerase chain reaction (PCR) was used to identify the genetic coding for interleukin-24, RANTES and CCR5. Genotype and allele frequencies were compared between the test and control groups using Fischer's exact test at the 5% level of significance.
  
  
  RESULTS
  There were no statistically significant differences between patients with chronic periodontitis and control subjects, regarding genotype distribution or allele frequency, irrespective of smoking status, in the combined Glasgow and Gothenburg cohort or in the specific location cohorts. The allele frequencies for healthy and control subjects for RANTES gave a p-value of 0.80 (allele G was 58.8% in healthy subjects and and 54.4% in subjects with periodontitis), for interleukin-24 the p-value was 0.90 (allele T was 56.2% in healthy subjects and and 54.9% in subjects with periodontitis) and for CCR5 the p-value was 0.90 (the wild-type allele was 85% in healthy subjects and and 82.7% in subjects with periodontitis).
  
  
  CONCLUSIONS
  The interleukin-24, RANTES and CCR5 polymorphisms investigated are not associated with chronic periodontitis.
authors.affiliation: University of Glasgow, Glasgow, UK.
authors.forename: L
authors.initials: L
authors.lastname: Savarrio
authors.forename: M
authors.initials: M
authors.lastname: Donati
authors.forename: C
authors.initials: C
authors.lastname: Carr
authors.forename: D F
authors.initials: DF
authors.lastname: Kinane
authors.forename: T
authors.initials: T
authors.lastname: Berglundh
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D018946:Chemokine CCL5 / Q000235:genetics*; D016009:Chi-Square Distribution; D002908:Chronic Disease; D005260:Female; D005787:Gene Frequency; D006801:Humans; D007378:Interleukins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000097:blood / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D019713:Receptors, CCR5 / Q000235:genetics*; D016037:Single-Blind Method; D000099180:Interleukin-24
chemical_list: D018946:Chemokine CCL5; D007378:Interleukins; D019713:Receptors, CCR5; D000099180:Interleukin-24