Schistosoma mansoni antigen-driven interleukin-10 production in infected asthmatic individuals.
Asthmatics infected with Schistosoma mansoni have a less severe course of asthma and an inhibition of the Th2 inflammatory response that seems to be mediated by interleukin (IL-10). The objective of this study was to evaluate the capacity of some S. mansoni antigens to stimulate IL-10 production in vitro by cells of asthmatic infected individuals. Peripheral bloods mononuclear cells were stimulated with the S. mansoni recombinant antigens Sm22.6, Sm14, P24, and PIII antigen. IL-10 was measured in the supernatants of cultures. As the recombinant antigens were cloned in Escherichia coli, we blocked contaminant endotoxin with polymyxin B added to the cultures. We demonstrated that all antigens used drove high production of IL-10 in S. mansoni infected individuals (n = 13, 408 +/- 514 and 401 +/- 383 pg/ml, 484 +/- 245 pg/ml, 579 +/- 468 pg/ml, respectively). In asthmatics infected with S. mansoni (n = 21) rP24 induced higher levels of IL-10 (565 +/- 377 pg/ml) when compared to PIII, rSm14 and rSm22.6 (184 +/- 209 pg/ml; 292 +/- 243 pg/ml; 156 +/- 247 pg/ml, respectively).


CONCLUSIONS
the S. mansoni antigens evaluated in this study stimulated IL-10 production by cells from infected individuals and therefore they have the potential to be used as a modulator of the inflammatory response in asthma.
Cardoso
Luciana S
LS
Serviço de Imunologia, Hospital Universitário Professor Edgard Santos, Universidade Federal da Bahia, 40110-160 Salvador, BA, Brazil.
Oliveira
Sergio C
SC
Pacífico
Lucila G G
LG
Góes
Alfredo M
AM
Oliveira
Ricardo R
RR
Fonseca
Cristina T
CT
Carvalho
Edgar M de
EM
Araújo
Maria Ilma
MI
D000293:Adolescent; D000328:Adult; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D001249:Asthma / Q000150:complications / Q000276:immunology* / Q000469:parasitology; D002478:Cells, Cultured; D002648:Child; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000469:parasitology; D008297:Male; D011112:Polymyxin B / Q000494:pharmacology; D011994:Recombinant Proteins / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000150:complications / Q000276:immunology*
D000947:Antigens, Helminth; D011994:Recombinant Proteins; D016753:Interleukin-10; D011112:Polymyxin B