[The effect of icariin and astragalosid I on the proliferation and differentiation of bone marrow stromal cells].
OBJECTIVE
To observe the effects of icariin and astragalosid I on the proliferative and alkaline phosphatase (ALP) activity of dog bone marrow stromal cells (BMSCs).


METHODS
The dog's BMSCs were isolated and cultured in vitro. The 3th generation BMSCs were treated with icariin or astragalosid I at the concentration of 50 ng/ml and compared with BMSCs of BMP-2 group and control group. The growth curves of BMSCs were drawn by 3-(4,5-dimiethylthiazole-2-yl)-2, 5-hiphenyl tetrazolium bromide (MTT) colorimetric assay every day from the 1st to the 8th day to estimate the proliferative ability of BMSCs. The curves of OD value of ALP excreted by BMSCs on the 1st, 3th, 6th, 10th and the 14th day were recorded to estimate the ALP activity of BMSCs.


RESULTS
After the pertreatment with icariin and astragalosid I, the BMSCs acquired higher MTF values and higher ALP's OD values as compared with control group and the difference between experiment group and control group was statistically significant (P < 0.05).


CONCLUSIONS
Icariin and Astragealosid I can accelerate the proliferation and ALP excretion of BMSCs. At the same time, the osteogenesis ability of these cells is greatly improved.
Liu
Hai-jiang
HJ
The College of Stomatology, Tongji University, Shanghai 200072, China.
Wang
Xiao-ping
XP
Lin
Juan
J
Yu
You-cheng
YC
Jiang
Xin-quan
XQ
Zhang
Xiu-li
XL
Zhou
Zeng-tong
ZT
Zhang
Wei-dong
WD
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D027885:Astragalus propinquus / Q000737:chemistry; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004285:Dogs; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005419:Flavonoids / Q000494:pharmacology*; D010012:Osteogenesis / Q000187:drug effects; D010946:Plants, Medicinal / Q000737:chemistry; D012503:Saponins / Q000494:pharmacology*; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D014315:Triterpenes / Q000494:pharmacology*
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D012503:Saponins; D014315:Triterpenes; C052064:astragaloside A; D000469:Alkaline Phosphatase; C056599:icariin