Modular design for in vivo optical imaging and ultrasound treatment in the murine brain.
Simultaneous optical imaging and ultrasound exposure to the brain is challenging due to geometric constraints. We demonstrate sonication through the ventral surface of the mouse, leaving the dorsal aspect free for imaging. Ultrasound components are integrated into a commercial multiphoton microscope, and animals are imaged during ultrasound-induced blood-brain barrier disruption.
Raymond
Scott B
SB
Skoch
Jesse
J
Bacskai
Brian J
BJ
Hynynen
Kullervo
K
D000818:Animals; D001812:Blood-Brain Barrier / Q000166:cytology* / Q000528:radiation effects*; D001921:Brain / Q000166:cytology* / Q000528:radiation effects*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation* / Q000379:methods / Q000662:veterinary; D013010:Sonication / Q000295:instrumentation*; D018511:Systems Integration; D014464:Ultrasonic Therapy / Q000295:instrumentation* / Q000379:methods / Q000662:veterinary