title: Correlation of ultrasonographic appearance of lesions and cytologic and histologic diagnoses in splenic aspirates from dogs and cats: 32 cases (2002-2005).
abstract: OBJECTIVE
  To determine the accuracy of cytologic diagnosis, compared with histologic diagnosis, in determination of disease in ultrasound-guided fine-needle aspirates of splenic lesions.
  
  
  METHODS
  Retrospective study.
  
  
  METHODS
  Splenic specimens from 29 dogs and 3 cats.
  
  
  METHODS
  Records were searched for dogs and cats that had undergone ultrasound-guided splenic aspiration. Criteria for inclusion were ultrasonographic identification of splenic lesions and cytologic and histologic evaluation of tissue from the same lesion. Cytologic samples were obtained by fine-needle aspiration, and histologic specimens were obtained via surgical biopsy, ultrasound-guided biopsy, or necropsy.
  
  
  RESULTS
  Cytologic diagnoses corresponded with histologic diagnoses in 19 of 31 (61.3%) cases and differed in 5 of 31(16.1%) cases, and 1 aspirate was inadequate for evaluation. In 7 of 31 (22.6%) cases, histologic evaluation of tissue architecture was required to distinguish between reactive and neoplastic conditions. On the basis of histologic diagnosis in 14 animals with nonneoplastic conditions, the cytologic diagnosis was correct in 11 cases, not definitive in 2 cases, and incorrect in 1 case. In 17 animals with malignant neoplastic diseases, the cytologic diagnosis was correct in 8 cases, not definitive but consistent with possible neoplasia in 5 cases, and incorrect in 4 cases. Multiple similar-appearing nodules were significantly associated with malignancy, whereas single lesions were more often benign.
  
  
  CONCLUSIONS
  Ultrasound-guided aspiration of splenic lesions is a minimally invasive tool for obtaining specimens for cytologic evaluation. Although cytologic diagnoses often reflect histologic results, if missampling or incomplete sampling occurs or tissue architecture is required to distinguish between reactive and neoplastic conditions, accurate diagnosis with fine-needle aspiration may not be possible.
authors.affiliation: Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706, USA.
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Ballegeer
authors.forename: Lisa J
authors.initials: LJ
authors.lastname: Forrest
authors.forename: Ryan M
authors.initials: RM
authors.lastname: Dickinson
authors.forename: Melissa M
authors.initials: MM
authors.lastname: Schutten
authors.forename: Fern A
authors.initials: FA
authors.lastname: Delaney
authors.forename: Karen M
authors.initials: KM
authors.lastname: Young
mesh_terms: D000818:Animals; D044963:Biopsy, Fine-Needle / Q000379:methods / Q000662:veterinary*; D002371:Cat Diseases / Q000175:diagnosis* / Q000000981:diagnostic imaging / Q000473:pathology; D002415:Cats; D003584:Cytological Techniques / Q000379:methods / Q000662:veterinary*; D003937:Diagnosis, Differential; D004283:Dog Diseases / Q000175:diagnosis* / Q000000981:diagnostic imaging / Q000473:pathology; D004285:Dogs; D005260:Female; D008297:Male; D012189:Retrospective Studies; D013158:Splenic Diseases / Q000175:diagnosis / Q000000981:diagnostic imaging / Q000473:pathology / Q000662:veterinary*; D014463:Ultrasonography