[Effect of endogeneous gangliosides on integrin alpha2beta1-mediated adhesion of neuroblastoma cells to collagen].
OBJECTIVE
To study the effect of endogeneous gangliosides (Gls) on integrin alpha2beta1-mediated adhesion of neuroblastoma cells to collagen (Col).


METHODS
Neuroblastoma SK-N-SH cell line was cultured in the modified eagle's medium with the presence of 10 mum D-threo-1-phenyl-2-decanolamino-3-morphinolin-1-propanol (D-PDMP), an inhibitor of glucosylceramide synthase. Flow cytometry was used to detect the expression of integrin alpha2beta1 in the cell line. The effects of Mg2(+) and monoclonal antibodies to integrin alpha2beta1 on the adhesion of the cell line to immobilized Col were observed. The adhesion cell number was measured with the BCA method and presented with absorptance A570.


RESULTS
There was a high expression of integrin alpha2beta1 in the SK-N-SH cell line without D-PDMP treatment. Endogenous Gls in the cells were almost depleted after 6-day exposure to D-PDMP, but the integrin alpha2beta1 expression was not significantly changed. 1 mmoL/L Mg2(+) treatment increased significantly the number of adhesion cells in the SK-N-SH cell line. The adhesion to Col of the SK-N-SH cells exposed to D-PDMP which Gls was depleted was significantly reduced compared with the control SK-N-SH cells treated with 1 mmoL/L Mg2(+) (A570: 0.33 +/- 0.016 vs 0.57 +/- 0.033; P < 0.01). After endogeneous Gls was added into the Gls-depleted SK-N-SH cells, the adhesion of the cells was restored (A570: 0.52 +/- 0.035). The adhesion of SK-N-SH cells was significantly blocked by anti-alpha2 and anti-beta1 monoclonal antibodies, with A570 of 0.31 +/- 0.018 and 0.36 +/- 0.021 respectively.


CONCLUSIONS
Endogenous tumor Gls increases neuroblastoma cell adhesion to Col by regulating the function of integrin alpha2beta1, but has no effects on the integrin expression. It is suggested that tumor Gls may play a role in migration, invasion and metastasis of tumor cells.
Liu
Zhi-Ping
ZP
Department of Pediatrics, Second Clinical College of Medicine, Jinan University, Shenzhen, Guangdong 518020, China.
Wen
Fei-Qiu
FQ
Chen
Yi-Xin
YX
Wang
Sha-Yan
SY
Zhou
Ke-Ying
KY
Xia
Quan
Q
D000911:Antibodies, Monoclonal / Q000276:immunology; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D003094:Collagen / Q000502:physiology*; D005732:Gangliosides / Q000502:physiology*; D006801:Humans; D038982:Integrin alpha2beta1 / Q000502:physiology*; D008274:Magnesium / Q000494:pharmacology; D009025:Morpholines / Q000494:pharmacology; D009447:Neuroblastoma / Q000473:pathology*
D000911:Antibodies, Monoclonal; D005732:Gangliosides; D038982:Integrin alpha2beta1; D009025:Morpholines; C033110:RV 538; D003094:Collagen; D008274:Magnesium