Early-phase endotoxic shock-induced myocardial injury increases iNOS and selectin expression in macaque primate.
BACKGROUND
Our previous study has established a macaque model with early-phase endotoxic shock. The present study further investigated myocardial and blood vessel injury in Macaques by examining the subsequent expression of ACP, selectins, iNOS, and cTnI in response to LPS treatment.


METHODS
In an experiment with anaesthetised, instrumental macaques, eleven animals were randomised into: an En group (n=6), receiving a dose of 2.8 mg kg(-1) lipopolysaccharides (LPS) by i.v.; and a Co group (n=5), injected with normal saline of 1 ml kg(-1). Cytochemistry of acid phosphatase (ACPase) in heart was performed by electron microscope at 120 min following endotoxin injection. Three immunochemical stains, namely, L-selectin, P-selectin and iNOS protein in heart, were studied. In addition, cardiac troponin I (cTnI), L-selectin and P-selectin in plasma were detected.


RESULTS
In the early phase of endotoxic shock, LPS caused myocardial lysosome damage. The data of immunochemical staining showed the thrombus formation in vessels and the increase of iNOS, L-Selectin and P-Selectin expression in heart, but LPS challenge did not change L-selectin, P-selectin and cTnI in plasma.


CONCLUSIONS
The increase of iNOS, L-selectin and P-selectin protein expression following endotoxin administration may have caused vessel injury and myocardial damage in macaques.
Yin
Guo Qing
GQ
College of Veterinary Medicine, Nanjing Agricultural University, 6 Tongwei Road, Nanjing, Jiangsu 210095, PR China. yinguoq@jlonline.com
Du
Kai He
KH
Gu
Fei Rong
FR
Fang
Zhi Xun
ZX
Tang
Jia Qi
JQ
Zhong
Bei
B
Zhu
Xiao Yun
XY
Wu
Ying Wei
YW
Lu
Cheng Ping
CP
D000818:Animals; D004195:Disease Models, Animal; D004926:Escherichia coli; D005260:Female; D019041:L-Selectin / Q000378:metabolism*; D008070:Lipopolysaccharides; D008251:Macaca; D008297:Male; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D019007:P-Selectin / Q000378:metabolism*; D012772:Shock, Septic / Q000150:complications* / Q000378:metabolism / Q000473:pathology; D019210:Troponin I / Q000097:blood
D008070:Lipopolysaccharides; D019007:P-Selectin; D019210:Troponin I; D019041:L-Selectin; D052247:Nitric Oxide Synthase Type II