Simultaneously determination of five ginsenosides in rabbit plasma using solid-phase extraction and HPLC/MS technique after intravenous administration of 'SHENMAI' injection.
In this study, a sensitive and reliable analytical method for the simultaneous determination of five ginsenosides (R(g1), R(f), R(e), R(d) and R(b1)) in rabbit plasma was developed by high performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-ESI-MS). Chromatographic separation was carried out on a Zorbax SB-C18 Column (150 mm x 2.1 mm i.d., 5.0 microm particle size) with a simple linear gradient elution. The detection was conducted on a single quadrupole mass spectrometer by selected ion monitoring mode via electrospray ionization source. Good linearity over the investigated concentration ranges was observed with the values of R(2) higher than 0.991 for all the analytes. Limits of detection of the analytes varied from 0.25 ng/ml to 1.45 ng/ml, and the average recoveries, examined at three concentration levels, ranged from 90.6% to 106.9%. The validated method was successfully applied to the determination of the ginsenosides in the rabbit plasma after intravenous administration of 'SHENMAI' injection.
Yu
Ke
K
Department of Chinese Medicine Science and Engineering, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
Ma
Yanhong
Y
Shao
Qing
Q
Qu
Haibin
H
Cheng
Yiyu
Y
D000818:Animals; D002138:Calibration; D002491:Central Nervous System Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics*; D036145:Ginsenosides / Q000097:blood*; D007202:Indicators and Reagents; D007275:Injections, Intravenous; D013058:Mass Spectrometry; D011786:Quality Control; D011817:Rabbits; D012015:Reference Standards; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization
D002491:Central Nervous System Agents; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; D007202:Indicators and Reagents; C009252:fructus schizandrae, radix ginseng, radix ophiopogonis drug combination