Immunohistochemical evaluation of substance P and calcitonin gene-related peptide in skin and periosteum after extracorporeal shock wave therapy and radial pressure wave therapy in sheep.
OBJECTIVE
To evaluate the effect of focused extracorporeal shock wave therapy (ESWT) and radial pressure wave therapy (RPWT) on immunohistochemical staining for substance P and calcitonin gene-related peptide (CGRP) in the skin and periosteum of sheep.


METHODS
36 sheep.


METHODS
All 4 limbs of 36 sheep were treated with ESWT, RPWT, or a sham treatment. For 14 days after treatment, at least 2 sheep were euthanized daily and tissue was harvested for histologic evaluation of nerves via staining for substance P and CGRP in the skin and periosteum.


RESULTS
No effects of ESWT or RPWT were observed on the number of nerves with stain uptake for substance P or CGRP in the skin or periosteum.


CONCLUSIONS
Substance P- and CGRP-containing nerve fibers are not disrupted by EWST or RPWT. Further studies are needed to identify the mechanism of analgesia observed in association with these treatment modalities.
Abed
Janan M
JM
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.
McClure
Scott R
SR
Yaeger
Michael J
MJ
Evans
Richard B
RB
D000698:Analgesia / Q000379:methods / Q000662:veterinary; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis* / Q000378:metabolism; D019220:High-Energy Shock Waves / Q000009:adverse effects* / Q000627:therapeutic use*; D007150:Immunohistochemistry / Q000662:veterinary; D009474:Neurons / Q000378:metabolism; D010521:Periosteum / Q000378:metabolism*; D011312:Pressure; D012756:Sheep / Q000502:physiology*; D012867:Skin / Q000378:metabolism*; D013373:Substance P / Q000032:analysis* / Q000378:metabolism; D013997:Time Factors
D013373:Substance P; D015740:Calcitonin Gene-Related Peptide