Apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G: a possible role in the resistance to HIV of HIV-exposed seronegative individuals.
Apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G (APOBEC3G), a human cytidine deaminase, is a potent inhibitor of HIV replication. To explore a possible role of this protein in modulating in vivo susceptibility to HIV infection, we analyzed APOBEC3G expression in HIV-exposed seronegative individuals, HIV-seropositive patients, and healthy control subjects. The results showed that the expression of APOBEC3G is significantly increased in peripheral blood mononuclear cells (PBMCs)--mainly CD14(+) cells--and in cervical tissues of HIV-exposed seronegative individuals. Higher APOBEC3G expression correlated with a reduced susceptibility of PBMCs to in vitro infection with the HIV-1(Ba-L) R5 strain. APOBEC3G could be important in modulating in vivo susceptibility to sexually transmitted HIV infection.
Biasin
Mara
M
Department of Immunology, Dipartimento di Scienze Precliniche Laboratorio Interdisciplinare Tecnologie Avanzate Vialba, Milano University Medical School, Milano, Italy.
Piacentini
Luca
L
Lo Caputo
Sergio
S
Kanari
Yasuyoshi
Y
Magri
Giuliana
G
Trabattoni
Daria
D
Naddeo
Valentina
V
Lopalco
Lucia
L
Clivio
Alberto
A
Cesana
Eugenio
E
Fasano
Francesca
F
Bergamaschi
Cristina
C
Mazzotta
Francesco
F
Miyazawa
Masaaki
M
Clerici
Mario
M
D000071479:APOBEC-1 Deaminase; D016022:Case-Control Studies; D003564:Cytidine Deaminase / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015658:HIV Infections / Q000097:blood / Q000821:virology*; D006679:HIV Seropositivity; D015497:HIV-1 / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D017393:RNA Editing; D012333:RNA, Messenger / Q000032:analysis; D014779:Virus Replication / Q000502:physiology
D012333:RNA, Messenger; D000071479:APOBEC-1 Deaminase; C000605089:APOBEC1 protein, human; D003564:Cytidine Deaminase