Moderation of the platelet releasate response by aspirin.
Modulation of the proteins released during activation is one mechanism whereby aspirin may influence platelet-mediated human disease. We investigated the effect of aspirin on the platelet releasate using mass spectrometry and found that different agonists evoked different releasate profiles, with aspirin having a general moderating effect on the amount of protein released regardless of the agonist. These observations were confirmed for several cytokines using an antibody array approach.
Coppinger
Judith A
JA
Department of Cell Biology, Scripps Research Institute, La Jolla, CA, USA.
O'Connor
Roisin
R
Wynne
Kieran
K
Flanagan
Michelle
M
Sullivan
Matthew
M
Maguire
Patricia B
PB
Fitzgerald
Desmond J
DJ
Cagney
Gerard
G
D001241:Aspirin / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism*; D003094:Collagen / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis; D005786:Gene Expression Regulation; D006801:Humans; D007124:Immunoenzyme Techniques; D007249:Inflammation; D013058:Mass Spectrometry / Q000379:methods; D010974:Platelet Aggregation; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D019699:Thrombospondins / Q000096:biosynthesis; D013929:Thromboxane B2 / Q000096:biosynthesis; D013997:Time Factors
D016207:Cytokines; D010975:Platelet Aggregation Inhibitors; D019699:Thrombospondins; D013929:Thromboxane B2; D003094:Collagen; D001241:Aspirin