RNA electrophoretic mobility shift assay using a fluorescent DNA sequencer.
The RNA electrophoretic mobility shift assay is a simple and rapid method for visualizing the existence of specific RNA-protein interaction. We have developed a useful method for the detection of mRNA-binding proteins using fluorescence-labeled synthetic RNA. In this method, RNA was prepared by in vitro transcription using Texas Red-labeled nucleotides. This method has higher resolution than the conventional method using slab gels, is safer, and provides results rapidly because of the use of fluorescence detection.
Eguchi
Yukinori
Y
Research Laboratory, Faculty of Medicine, University of Ryukyus, Okinawa, Japan.
D000818:Animals; D001483:Base Sequence; D001854:Bone Marrow Cells / Q000378:metabolism; D002645:Chickens; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D024202:Electrophoretic Mobility Shift Assay / Q000379:methods*; D005456:Fluorescent Dyes; D021141:Nucleic Acid Amplification Techniques; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic; D014966:Xanthenes
D017931:DNA Primers; D018076:DNA, Complementary; D005456:Fluorescent Dyes; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D014966:Xanthenes; C034657:Texas red