title: In vitro osteogenesis on a highly bioactive glass-ceramic (Biosilicate).
abstract: One of the strategies to improve the mechanical performance of bioactive glasses for load-bearing implant devices has been the development of glass-ceramic materials. The present study aimed to evaluate the effect of a highly bioactive, fully-crystallized glass-ceramic (Biosilicate) of the system P(2)O(5)-Na(2)O-CaO-SiO(2) on various key parameters of in vitro osteogenesis. Surface characterization was carried out by scanning electron microscopy and Fourier transform infrared spectroscopy. Osteogenic cells were obtained by enzymatic digestion of newborn rat calvarial bone and by growing on Biosilicate discs and on control bioactive glass surfaces (Biosilicate) parent glass and Bioglass(R) 45S5) for periods of up to 17 days. All materials developed an apatite layer in simulated body fluid for 24h. Additionally, as early as 12 h under culture conditions and in the absence of cells, all surfaces developed a layer of silica-gel that was gradually covered by amorphous calcium phosphate deposits, which remained amorphous up to 72 h. During the proliferative phase of osteogenic cultures, the majority of cells exhibited disassembly of the actin cytoskeleton, whereas reassembly of actin stress fibers took place only in areas of cell multilayering by day 5. Although no significant differences were detected in terms of total protein content and alkaline phosphatase activity at days 11 and 17, Biosilicate supported significantly larger areas of calcified matrix at day 17. The results indicate that full crystallization of bioactive glasses in a range of compositions of the system P(2)O(5)-Na(2)O-CaO-SiO(2) may promote enhancement of in vitro bone-like tissue formation in an osteogenic cell culture system.
authors.affiliation: Cell Culture Laboratory, Faculty of Dentistry of Ribeirão Preto, University of São Paulo, Av. do Café, s/n, CEP 14040-904, Ribeirão Preto, São Paulo, Brazil.
authors.forename: João
authors.initials: J
authors.lastname: Moura
authors.forename: Lucas Novaes
authors.initials: LN
authors.lastname: Teixeira
authors.forename: Christian
authors.initials: C
authors.lastname: Ravagnani
authors.forename: Oscar
authors.initials: O
authors.lastname: Peitl
authors.forename: Edgar Dutra
authors.initials: ED
authors.lastname: Zanotto
authors.forename: Márcio Mateus
authors.initials: MM
authors.lastname: Beloti
authors.forename: Heitor
authors.initials: H
authors.lastname: Panzeri
authors.forename: Adalberto Luiz
authors.initials: AL
authors.lastname: Rosa
authors.forename: Paulo Tambasco
authors.initials: PT
authors.lastname: de Oliveira
mesh_terms: D000818:Animals; D001031:Apatites; D018786:Bone Substitutes; D001842:Bone and Bones / Q000166:cytology*; D002113:Calcification, Physiologic; D002130:Calcium Phosphates; D002478:Cells, Cultured; D002516:Ceramics; D010012:Osteogenesis; D051381:Rats; D017640:Silicates; D022502:Stress Fibers; D013499:Surface Properties
chemical_list: D001031:Apatites; C022107:Bioglass; D018786:Bone Substitutes; D002130:Calcium Phosphates; D017640:Silicates; C519480:amorphous calcium phosphate