Differentiation of Entamoeba histolytica and Entamoeba dispar by PCR: a preliminary study in Izmir, Turkey.
The causative agent of amoebiasis is currently attributed to two distinct species (E. histolytica and E. dispar). The aim of this study was to differentiate these species by PCR in stool samples. Isolated genomic DNA was amplified by PCR and band products of 101 bp (E. dispar) were obtained. All seven stool samples were found to be E. dispar, not E. histolytica. Our results demonstrated the significance of E. histolytica/dispar differentiation in the diagnosis of amoebiasis. This study is preliminary to our current research project entitled "Investigation of the prevalence of amoebiasis and Entamoeba species in Izmir and its hinterland".
Dagci
Hande
H
Ege University School of Medicine, Department of Parasitology, Izmir, Turkey. hande.dagci@ege.edu.tr
Erdogan
Derya Dirim
DD
Toz
Seray Ozensoy
SO
Kurt
Ozgur
O
Ustun
Sebnem
S
Akarca
Ulus
U
D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis / Q000302:isolation & purification; D004747:Entamoeba / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004748:Entamoeba histolytica / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004749:Entamoebiasis / Q000175:diagnosis* / Q000469:parasitology*; D005243:Feces / Q000469:parasitology; D016133:Polymerase Chain Reaction / Q000379:methods*; D014421:Turkey
D016054:DNA, Protozoan