title: The transfusion of neutrophil-specific antibodies causes leukopenia and a broad spectrum of pulmonary reactions.
abstract: BACKGROUND
  Antibodies to neutrophil-specific antigens are the best characterized cause of transfusion-related acute lung injury (TRALI).
  
  
  METHODS
  A double-apheresis platelet (PLT) component was divided and transfused into two patients. One experienced chills, rigors, and dyspnea and the other experienced chills and headache. Transient leukopenia developed in both patients.
  
  
  RESULTS
  Evaluation of donor plasma revealed an anti-HNA-2a and no HLA Class I antibodies. The donor had donated 26 previous apheresis PLT components. The 27 donations resulted in 39 separate transfusions and 12 transfusion reactions in 9 patients. Five reactions occurred immediately after the transfusion, 10 within 1 hour, and all within 2.5 hours. Nine of the reactions involved symptoms or signs of pulmonary dysfunction. The symptoms were mild to moderate in nature. None of the inpatients required intensive care transfer nor did any outpatients require hospital admission. Recipient white blood cell (WBC) counts were measured within 8 hours after 38 of 39 transfusions. Leukopenia occurred in 9 of 12 (75%) transfusions with reactions and in 9 of 26 (35%) transfusions without. The reactions did not correlate with pretransfusion WBC count.
  
  
  CONCLUSIONS
  Neutrophil antibodies cause a wide variety of transfusion reactions that do not necessarily meet the definition of TRALI. Donors of blood products causing even mild pulmonary reactions or leukopenia should be tested for neutrophil-specific antibodies.
authors.affiliation: Department of Transfusion Medicine, Clinical Center, and the Pediatric Oncology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-1184, USA.
authors.forename: Emmanuel A
authors.initials: EA
authors.lastname: Fadeyi
authors.forename: Maria
authors.initials: M
authors.lastname: De Los Angeles Muniz
authors.forename: Alan S
authors.initials: AS
authors.lastname: Wayne
authors.forename: Harvey G
authors.initials: HG
authors.lastname: Klein
authors.forename: Susan F
authors.initials: SF
authors.lastname: Leitman
authors.forename: David F
authors.initials: DF
authors.lastname: Stroncek
mesh_terms: D000293:Adolescent; D000328:Adult; D019268:Antibodies, Antineutrophil Cytoplasmic; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D007970:Leukopenia / Q000209:etiology*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000276:immunology*; D012128:Respiratory Distress Syndrome / Q000209:etiology*; D065227:Transfusion Reaction
chemical_list: D019268:Antibodies, Antineutrophil Cytoplasmic