title: Real-time PCR fluorescent chemistries.
abstract: There are more than a dozen formats available for the fluorescent detection of amplified DNA in kinetic (real-time) PCR. These chemistries are adaptable to most real-time PCR instruments and may offer benefits over the usual manufacturer-recommended chemistries for the instrument. The most popular chemistries are the generic dye, SYBR Green I, TaqMan, and hybridization probes. However, there are now new dyes being reported with superior fluorescent detection and product resolution; as well as new probe formats that may offer advanced multiplexing opportunities for quantification and genotyping.
authors.affiliation: Applied Science, Roche Diagnostics, Mt Wellington, Auckland, New Zealand.
authors.forename: John
authors.initials: J
authors.lastname: Mackay
authors.forename: Olfert
authors.initials: O
authors.lastname: Landt
mesh_terms: D052160:Benzothiazoles; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D003959:Diamines; D005456:Fluorescent Dyes / Q000737:chemistry*; D006868:Hydrolysis; D007700:Kinetics; D015336:Molecular Probe Techniques; D015345:Oligonucleotide Probes; D009930:Organic Chemicals; D016133:Polymerase Chain Reaction / Q000379:methods*; D011804:Quinolines; D013816:Thermodynamics
chemical_list: D052160:Benzothiazoles; D017931:DNA Primers; D003959:Diamines; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I; D004247:DNA