Detection of nodavirus in seawater from rearing facilities for Atlantic halibut Hippoglossus hippoglossus larvae.
We used (1) ultracentrifugation followed by RT-PCR and (2) real-time RT-PCR to detect and quantify nodaviruses in seawater in which Atlantic halibut Hippoglossus hippoglossus larvae/fry had been held at rearing facilities. Evaluated against in vitro propagated viruses, the viral concentration corresponded to 1.6 x 10(4) TCID50 (50% tissue culture infectious dose) ml(-1). Evaluated against in vitro transcribed RNA, the concentration was estimated at 2 x 10(7) virus particles ml(-1) seawater.
Nerland
Audun H
AH
Institute of Marine Research, PO Box 1870 Nordnes, 5817 Bergen, Norway. audun.nerland@imr.no
Skaar
Cecilie
C
Eriksen
Tove Boge
TB
Bleie
Hogne
H
D000818:Animals; D005393:Fish Diseases / Q000635:transmission / Q000821:virology; D005398:Fisheries / Q000379:methods*; D005432:Flounder / Q000821:virology*; D007814:Larva / Q000821:virology; D030381:Nodaviridae / Q000302:isolation & purification*; D012327:RNA Virus Infections / Q000635:transmission / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012623:Seawater / Q000821:virology*; D014461:Ultracentrifugation / Q000379:methods / Q000662:veterinary; D014871:Water Microbiology