title: Pilocarpine-loaded chitosan-PAA nanosuspension for ophthalmic delivery.
abstract: Chitosan-poly(acrylic acid) (CS-PAA) nanoparticles, to be used as ophthalmic drug carrier, were successfully prepared using template polymerization of acrylic acid (AA) in a chitosan solution. When the polymerization was done at 70 degrees C for 45 min with a CS/AA weight ratio of 1:1, the surface structure of the prepared nanoparticles was most stable with the smallest mean diameter (92.0 +/- 7.5 nm) and a stable zeta potential (25.5 +/- 2.6 mV) in a buffer solution (pH 4.5). The size of the nanoparticles dramatically increased with the pH value of the medium. Both in vitro and in vivo studies revealed that the prepared nanoparticle suspension was better at sustaining the release of pilocarpine than either simulated tear fluid or commercial eye drops.
authors.affiliation: Department of Chemical and Material Engineering, Southern Taiwan University of Technology, Tainan 710, Taiwan. hrlin@mail.stut.edu.tw
authors.forename: Hong-Ru
authors.initials: HR
authors.lastname: Lin
authors.forename: Shi-Ping
authors.initials: SP
authors.lastname: Yu
authors.forename: Chun-Jung
authors.initials: CJ
authors.lastname: Kuo
authors.forename: Huei-Jen
authors.initials: HJ
authors.lastname: Kao
authors.forename: Yu-Li
authors.initials: YL
authors.lastname: Lo
authors.forename: Yiu-Jiuan
authors.initials: YJ
authors.lastname: Lin
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry; D004337:Drug Carriers; D005260:Female; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008297:Male; D008422:Materials Testing; D008916:Miotics / Q000008:administration & dosage* / Q000493:pharmacokinetics; D008970:Molecular Weight; D053758:Nanoparticles; D009883:Ophthalmic Solutions; D010316:Particle Size; D010862:Pilocarpine / Q000008:administration & dosage* / Q000493:pharmacokinetics; D011817:Rabbits; D017550:Spectroscopy, Fourier Transform Infrared; D013535:Suspensions
chemical_list: D000180:Acrylic Resins; D001672:Biocompatible Materials; D004337:Drug Carriers; D008916:Miotics; D009883:Ophthalmic Solutions; D013535:Suspensions; D010862:Pilocarpine; C065246:chitosan-poly(acrylic acid); D048271:Chitosan