title: [Axonal regeneration of retinal ganglion cells after optic nerve crush: experiment of Nogo-A knockout mice].
abstract: OBJECTIVE
  To investigate the role Nogo-A gene plays in the axonal regeneration of retinal ganglion cells (RGCs) after optic nerve crush.
  
  
  METHODS
  Twenty Nogo-A knockout C57BL mice and 20 normal C57BL/6 mice underwent clipping at the optic nerve 2 mm behind the eyeball by specially designed clip so as to cause partial optic nerve injury. Then the mice in each group were subdivided into subgroups of day 1 (n = 7), day 3 (n = 7), and day 7 (n = 6). The optical nerves of different groups were taken out to detect the mRNA expression of Nogo-A gene by in situ hybridization. Frozen sections of optical nerve were immunostained to investigate the expression of GAP-43, a protein showing axonal regeneration, by immunofluorescence assay. RGCs were cultured and immunostained with Thy1.1 antibody and GAP-43 antibody. The axonal growth of the RGCs was calculated with a computerized image analyzer.
  
  
  RESULTS
  Nogo-A expression could be seen in the optical nerves of the control mice, however, not in the Nogo-A knockout mice. The expression levels of GAP-43 at different time points of the Nogo-A knockout mice were all significantly higher than those of the control mice (t = 2.12, 3.56, 2.63, P < 0.01). Staining of GAP-43 antibody could be seen in the axons of the cultured RGCs. The neurite growth of the Nogo-A knockout mice was significantly longer than that of the control mice at different time points (F = 41.36, 31.23, P < 0.01).
  
  
  CONCLUSIONS
  Nogo-A gene plays an important role in inhibition of axonal regeneration of optic nerve after injury.
authors.affiliation: Department of Ophthalmology, First Clinical College, Harbin Medical University, Harbin 150001, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Su
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Shi-guang
authors.initials: SG
authors.lastname: Zhao
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Hao
authors.initials: H
authors.lastname: Cui
authors.forename: Yan
authors.initials: Y
authors.lastname: Teng
mesh_terms: D000818:Animals; D019922:GAP-43 Protein / Q000096:biosynthesis; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009185:Myelin Proteins / Q000096:biosynthesis* / Q000235:genetics; D009416:Nerve Regeneration; D000070798:Nogo Proteins; D009900:Optic Nerve / Q000378:metabolism / Q000503:physiopathology; D020221:Optic Nerve Injuries / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D019922:GAP-43 Protein; D009185:Myelin Proteins; D000070798:Nogo Proteins; D012333:RNA, Messenger; C000603201:Rtn4 protein, mouse