Role of the Synechococcus PCC 7942 nitrogen regulator protein PipX in NtcA-controlled processes.
The Synechococcus sp. PCC 7942 nitrogen regulator PipX interacts in a 2-oxoglutarate-dependent manner with the global nitrogen transcription factor NtcA and the signal transduction protein P(II). In vivo, PipX is involved in the NtcA-dependent induction of glnB and glnN genes. To further investigate the extent to which PipX is involved in global nitrogen control, the effect of pipX inactivation on various nitrogen-regulated processes was determined. The PipX-deficient mutant was able to use nitrate as a nitrogen source and to efficiently inhibit the nitrate transport upon ammonium addition but showed decreased nitrate and nitrite reductase activities and a delay in the induction of nitrate utilization after transfer of cultures from ammonium- to nitrate-containing media. In contrast to the wild-type, glutamine synthetase activity was not upregulated upon depletion of combined nitrogen from cultures of the mutant strain. Inactivation of pipX impaired induction of nblA and delayed phycobilisome degradation, but did not affect recovery of nitrogen-deprived cultures. Taken together, the results indicate that PipX interacts with NtcA to facilitate efficient acclimation of cyanobacteria to conditions of nitrogen limitation.
Espinosa
Javier
J
División de Genética, Universidad de Alicante, Apartado 99, E-03080 Alicante, Spain.
Forchhammer
Karl
K
Institut für Mikrobiologie und Molekularbiologie, Universität Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany.
Contreras
Asunción
A
División de Genética, Universidad de Alicante, Apartado 99, E-03080 Alicante, Spain.
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D050901:Nitrate Reductase / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism; D009572:Nitrite Reductases / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism*; D045524:Phycobilisomes / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D046940:Synechococcus / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D009566:Nitrates; D045524:Phycobilisomes; D000644:Quaternary Ammonium Compounds; C426123:nblA protein, Synechocystis; D009572:Nitrite Reductases; D050901:Nitrate Reductase; D005974:Glutamate-Ammonia Ligase; D009584:Nitrogen