title: [Osteogenic potential of platelet-rich plasma combined with cells and artificial bone].
abstract: OBJECTIVE
  To investigate the ability of platelet-rich plasma(PRP) combined with cells and artificial bone in accelerating the repair of bone defect.
  
  
  METHODS
  The marrow stromal stem cells (MSCs) of rabbit were cultured and induced into the osteoblast-like cells in vitro. PRP was produced with low-density twice centrifugations. Forty-eight New Zealand rabbits were made 1.2 cm bilateral radius defect models and divided into 4 groups averagely at random: group A (left:PRP/MSCs/beta-tricalcium phosphate (beta-TCP), right: MSCs/beta-TCP), group B (left:auto-radius, right: PRP/MSCs/beta-TCP); group C (left:auto-radius, right: MSCs/beta-TCP), and group D (left:PRP/beta-TCP; right:beta-TCP). At 2, 6 and 12 weeks after operation, the repair of bone defect was evaluated by the general observation, histology, biomechanics and histomorphology.
  
  
  RESULTS
  There was a stable platelet concentration in PRP and it was about 5.45+/-0. 23 times of whole blood. In the aspect of bone bridge and conture of the defects, at 2 and 6 weeks, PRP/MSCs/ beta-TCP and MSCs/beta-TCP displayed a similar outcome and were less than auto in general sample and X-ray; at 12 weeks, PRP/MSCs/beta-TCP was similar to auto-radius and better than MSCs/beta-TCP. in the aspect of quantity and quality of bone formation, histology showed that PRP/MSCs/beta-TCP and auto-radius were better than MSCs/beta-TCP (P < 0.05), and there was no significant difference between PRP/MSCs/beta-TCP and auto-radius (P>0.05). At 2 and 6 weeks, there was no significant difference between PRP/beta-TCP and beta-TCP (P>0.05). At 12 weeks, PRP/beta-TCP was better than beta-TCP (P<0.05). In the aspect of intensity of bone formation, at 6 and 12 weeks, PRP/MSCs/beta-TCP and auto-radius were better than MSCs/beta-TCP (P<0.05). At 6 weeks, auto-radius was better than PRP/MSCs/beta-TCP (P < 0.05). At 12 weeks, there was no significant difference between PRP/MSCs/beta-TCP and auto (P>0.05). PRP/TCP and beta-TCP had no significant difference at 12 weeks (P> 0.05).
  
  
  CONCLUSIONS
  PRP/MSCs/beta-TCP demonstrated excellent ability of forming bone in experiment. PRP was most likely to accelerate the repair of bone defect through increasing the activity of proliferation and differentiation of MSCs and osteoblasts.
authors.affiliation: Department of Orthopaedics, the Sixth People's Hospital of Shanghai, Shanghai Jiaotong University, Shanghai, 200233, P. R. China.
authors.forename: Sibo
authors.initials: S
authors.lastname: Li
authors.forename: Changqing
authors.initials: C
authors.lastname: Zhang
authors.forename: Ting
authors.initials: T
authors.lastname: Yuan
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D018786:Bone Substitutes; D016025:Bone Transplantation; D002130:Calcium Phosphates / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology*; D053657:Platelet-Rich Plasma; D011817:Rabbits; D011884:Radius / Q000293:injuries* / Q000601:surgery; D011897:Random Allocation; D023822:Tissue Engineering
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate