title: Halococcus qingdaonensis sp. nov., a halophilic archaeon isolated from a crude sea-salt sample.
abstract: A Gram-negative, extremely halophilic, coccoid archaeal strain, CM5(T), was isolated from a crude sea-salt sample collected near Qingdao, China. The organism grew optimally at 35-40 degrees C and pH 6.0 in the presence of 20 % (w/v) NaCl. Its colonies were red in colour and it could use glucose as a sole carbon source for growth. The 16S rRNA gene sequence of CM5(T) was most closely related to those of Halococcus species. Its pattern of antibiotic susceptibility was similar to those of other described Halococcus species. Biochemical tests revealed no sign of H(2)S production or gelatin liquefaction. The main polar lipids of strain CM5(T) were phosphatidylglycerol, phosphatidylglycerol methylphosphate and sulfated diglycosyl diether. No phosphatidylglycerol sulfate was present. The DNA G+C content of strain CM5(T) was 61.2 mol% and it gave DNA-DNA reassociation values of 33.7, 57.1 and 29.6 %, respectively, with Halococcus salifodinae DSM 8989(T), Halococcus dombrowskii DSM 14522(T) and Halococcus morrhuae ATCC 17082(T). Based on its morphological and chemotaxonomic properties and phylogenetic analysis of 16S rRNA gene sequence data, we propose that CM5(T) should be classified within a novel species, Halococcus qingdaonensis sp. nov., with strain CM5(T) (=CGMCC 1.4243(T)=JCM 13587(T)) as the type strain.
authors.affiliation: Institute of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou 730000, P. R. China.
authors.forename: Qian-Fu
authors.initials: QF
authors.lastname: Wang
authors.affiliation: Institute of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou 730000, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Institute of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou 730000, P. R. China.
authors.forename: Hai
authors.initials: H
authors.lastname: Yang
authors.affiliation: Institute of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou 730000, P. R. China.
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Liu
authors.affiliation: Institute of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou 730000, P. R. China.
authors.forename: Hai-Hua
authors.initials: HH
authors.lastname: Cao
authors.affiliation: Fachbereich Molekulare Biologie, Abt. Mikrobiologie, Billrothstrasse 11, A-5020 Salzburg, Austria.
authors.forename: Marion
authors.initials: M
authors.lastname: Dornmayr-Pfaffenhuemer
authors.affiliation: Fachbereich Molekulare Biologie, Abt. Mikrobiologie, Billrothstrasse 11, A-5020 Salzburg, Austria.
authors.forename: Helga
authors.initials: H
authors.lastname: Stan-Lotter
authors.affiliation: Institute of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou 730000, P. R. China.
authors.forename: Guang-Qin
authors.initials: GQ
authors.lastname: Guo
mesh_terms: D001482:Base Composition; D002681:China; D019641:DNA, Archaeal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D019625:Halococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D012965:Sodium Chloride
chemical_list: D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride