Simple colorimetric microplate test of phage lysis in Salmonella enterica.
A simple microplate method, based on conversion of tetrazolium to formazan, was devised for rapidly assessing Salmonella survival after phage treatment. Results were easily interpretable. Monitoring with a microplate reader was useful, but not required. The method was used in defining phage-Salmonella interactions for selection of phage biocontrol cocktails.
McLaughlin
M R
MR
United States Department of Agriculture, Agricultural Research Service, Waste Management, and Forage Research Unit, Mississippi State, Mississippi, 39762-5367, USA. mmclaughlin@ars.usda.gov
D003124:Colorimetry / Q000379:methods; D005562:Formazans / Q000032:analysis* / Q000378:metabolism; D012483:Salmonella Phages / Q000378:metabolism*; D019779:Salmonella enterica / Q000254:growth & development* / Q000821:virology*; D013778:Tetrazolium Salts / Q000737:chemistry* / Q000378:metabolism; D010948:Viral Plaque Assay
D005562:Formazans; D013778:Tetrazolium Salts; C009591:triphenyltetrazolium