[New container of sample: role in the reduction of bacterial contamination of standard platelet units].
BACKGROUND
Bacterial contamination of unstable blood products constitutes today the most frequent infectious risk transmitted by blood transfusion. Platelet concentrates are often incrimineted. As responsible germs are in general of cutaneous origin, a sample procedure with diversion of the first 20 ml during blood donation is studied. The aim of this study is to evaluate the results of this technique on bacterial contamination rate of standard platelet units prepared at the regional blood transfusion center in Casablanca.


METHODS
A comparative study of two types of sample pockets is made: 500 Standard Platelet concentrates (CPS) are prepared after collection using standard triple bags (Baxter) (group 1) and 560 pockets of CPS were prepared after collection using triple bags with Sample Diversion Pouch sampling system for elimination of the first 20 ml of donation (Macopharma and Terumo) (group 2). The skin was disinfected in two times with alcohol 70%. The bacteriological study was made in the two groups at the third day of conservation.


RESULTS
Six CPS of group 1 were contaminated, of which five were staphylococci coagulase negative and one bacillus sp. Six CPS of group 2 were contaminated, of which five were staphylococci coagulase negative and one staphylococcus aureus. The bacteria isolated were those of cutaneous flora at 100%. Diversion of first 20 ml of blood donation results in a 16.6% reduction in bacterial contamination of CPS (P>0.05).


CONCLUSIONS
The non-significant reduction in the prevalence of the bacterial infection of CP formulates the problem of the indication of the sampling devices with derivation of first 20 ml during blood collection.
Fehri
S
S
Centre régional de transfusion sanguine de Casablanca, rue Faidouzi, BP 5338 Maarif Casablanca, Maroc. fehrisiham@hotmail.fr
Tazi
I
I
Loukhmass
L
L
Benchemsi
N
N
D001407:Bacillus / Q000302:isolation & purification; D001424:Bacterial Infections / Q000517:prevention & control; D001792:Blood Platelets / Q000382:microbiology*; D001800:Blood Specimen Collection / Q000295:instrumentation* / Q000379:methods; D003030:Coagulase / Q000032:analysis; D004203:Disinfection / Q000379:methods; D006801:Humans; D017713:Platelet Transfusion / Q000295:instrumentation* / Q000592:standards; D012867:Skin / Q000382:microbiology; D013211:Staphylococcus aureus / Q000302:isolation & purification
D003030:Coagulase