title: Reduced iodide transport (stunning) and DNA synthesis in thyrocytes exposed to low absorbed doses from 131I in vitro.
abstract: Thyroid stunning refers to reduced uptake of (131)I in the thyroid tissue (or tumor) during radioiodine ((131)I) therapy compared with the uptake measured after the previous administration of (131)I for diagnostic purposes. The phenomenon is clinically important, as it can potentially lead to the undertreatment of thyroid cancer or to unnecessarily high absorbed doses in critical organs. Previous clinical and experimental studies indicated that thyroid stunning is absorbed dose dependent. The aim of this study was to investigate the effects of (131)I irradiation on (125)I(-) transport and cell proliferation at low absorbed doses in vitro.
  
  
  METHODS
  Primary cultured porcine thyroid cells were grown to form a confluent monolayer of epithelial cells on a filter in a bicameral culture system. The cells were continuously irradiated with (131)I in the culture medium for 48 h to obtain 0.0015-1.5 Gy. At 3 d after irradiation was stopped, the transepithelial iodide transport capacity was evaluated by measuring (125)I(-) transport from the basal chamber compartment to the apical chamber compartment. The effect of (131)I irradiation on DNA synthesis was estimated by pulse labeling with (3)H-thymidine of both subconfluent and confluent cells irradiated with up to 9 Gy. Total DNA content was measured to quantify cell numbers.
  
  
  RESULTS
  A statistically significant reduction in (125)I(-) transport was seen at absorbed doses of >or=0.15 Gy, with a 50% reduction at 1.5 Gy, compared with the results observed for nonirradiated control cells. (3)H-Thymidine incorporation was already statistically significantly reduced at absorbed doses of 0.01-0.1 Gy, but 0.15-0.3 Gy did not affect DNA synthesis. However, absorbed doses of >or=1 Gy again resulted in reduced DNA synthesis. A 50% reduction was obtained at 4 Gy. Total DNA measurements revealed a statistically significant reduction in cell numbers at 8 Gy.
  
  
  CONCLUSIONS
  The lowest absorbed dose from (131)I that reduced iodide transport was 0.15 Gy. Because stunning was found at low absorbed doses, it might occur for (131)I treatment not only of thyroid cancer but also of thyrotoxicosis. On the basis of differences in dose responses, radiation-induced thyroid stunning and cell cycle arrest may be independent phenomena.
authors.affiliation: Department of Radiation Physics, Göteborg University, Göteborg, Sweden. charlotta.lundh@radfys.gu.se
authors.forename: Charlotta
authors.initials: C
authors.lastname: Lundh
authors.forename: Madeleine M
authors.initials: MM
authors.lastname: Nordén
authors.forename: Mikael
authors.initials: M
authors.lastname: Nilsson
authors.forename: Eva
authors.initials: E
authors.lastname: Forssell-Aronsson
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D004247:DNA / Q000096:biosynthesis*; D007454:Iodides / Q000493:pharmacokinetics*; D007457:Iodine Radioisotopes / Q000627:therapeutic use*; D011829:Radiation Dosage; D013552:Swine; D013961:Thyroid Gland / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*
chemical_list: D007454:Iodides; D007457:Iodine Radioisotopes; D004247:DNA