Epstein Barr virus genotypes and LMP-1 variants in HIV-infected patients.
Two Epstein Barr virus (EBV) genotypes: EBV-1 and EBV-2 have been described. A 30-bp deletion in latent membrane protein-1 gene (del-LMP-1) has been identified in various pathologies. The aim of this study was to determine EBV genotypes and 30-bp deletion frequency in HIV-infected patients from Argentina. The study was performed on 258 individuals:


METHODS
144 HIV-infected patients that included: (a) 7 AIDS patients with primary central nervous system lymphoma (PCNSL), (b) 62 AIDS patients, and (c) 75 asymptomatic HIV-infected patients.


METHODS
114 HIV-negative individuals. EBV genotypes and variants in LMP-1 gene were detected by polymerase chain reaction (PCR)-Southern blot on DNA extracted from peripheral blood mononuclear cells and brain biopsies. In PCNSL, the presence of EBV was confirmed by EBER RNA in situ hybridization, and DNA sequencing of 3' end LMP-l gene of PCR products was performed. In HIV-infected patients, EBV-1 was detected in 48.6%, EBV-2 in 18.8%, and co-infection with both genotypes in 32.6%. In control group, EBV-1 was present in 74.3%, EBV-2 in 12.4%, and co-infection in 13.3%. Del-LMP-1 was found in 44.4% of HIV-infected patients samples (20.7% alone and 23.7% co-infection with non-deleted form) while it was found in 25.3% (6.3% alone and 19% with co-infection) in HIV-negative individuals. In HIV-infected patients EBV-2, co-infection and 30-bp deletion are more prevalent than in control group. In all, PCNSL brain biopsies samples, del-LMP-1 always was detected with EBV-2, but more cases would have to be included to draw definitive conclusions.
Correa
Rita Mariel
RM
Servicio Virus Oncogénicos, Departamento Virología, Instituto Nacional de Enfermedades Infecciosas (INEI)-ANLIS Dr. Carlos G. Malbrán, Buenos Aires, Argentina. oncovir2@anlis.gov.ar
Fellner
María Dolores
MD
Durand
Karina
K
Redini
Liliana
L
Alonio
Virginia
V
Yampolsky
Claudio
C
Colobraro
Antonio
A
Sevlever
Gustavo
G
Teyssié
Angélica
A
Benetucci
Jorge
J
Picconi
María Alejandra
MA
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications; D000293:Adolescent; D000328:Adult; D001118:Argentina; D001483:Base Sequence; D001706:Biopsy; D015139:Blotting, Southern; D001921:Brain / Q000473:pathology / Q000821:virology; D016543:Central Nervous System Neoplasms / Q000150:complications / Q000473:pathology; D020031:Epstein-Barr Virus Infections / Q000150:complications* / Q000821:virology*; D014644:Genetic Variation; D006678:HIV; D015658:HIV Infections / Q000150:complications*; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D016483:Lymphoma, AIDS-Related / Q000150:complications; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D017384:Sequence Deletion; D013045:Species Specificity; D014763:Viral Matrix Proteins / Q000235:genetics*
C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins