title: Expression of CD9 in frozen-thawed mouse oocytes: preliminary experience.
abstract: CD9 mRNA and protein expression levels in mouse slow frozen-rapid thawed oocytes were compared with those in fresh oocytes by using comparative quantitative real time reverse transcription-PCR and semiquantitative Western blot, respectively. The expression levels of both CD9 mRNA and protein in the frozen oocytes were significantly lower than those found in the fresh oocytes.
authors.affiliation: Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford, California 94305-5317, USA. yanwen@stanford.edu
authors.forename: Yan
authors.initials: Y
authors.lastname: Wen
authors.forename: Rudy
authors.initials: R
authors.lastname: Quintero
authors.forename: Bertha
authors.initials: B
authors.lastname: Chen
authors.forename: Yimin
authors.initials: Y
authors.lastname: Shu
authors.forename: Mary Lake
authors.initials: ML
authors.lastname: Polan
authors.forename: Barry
authors.initials: B
authors.lastname: Behr
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics* / Q000378:metabolism; D015925:Cryopreservation; D005260:Female; D005615:Freezing; D015870:Gene Expression; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009865:Oocytes / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism; D010865:Pilot Projects; D060245:Tetraspanin 29; D014018:Tissue Distribution
chemical_list: D015703:Antigens, CD; C554870:Cd9 protein, mouse; D008562:Membrane Glycoproteins; D060245:Tetraspanin 29