Long-term culture of porcine esophageal epithelial cells without use of feeder layers.
Epithelial cells from normal pig esophagus survived in culture for about 4 months undergoing about 12 passages and nearly 40 doublings before showing signs of slow proliferation and senescence. Epithelial cells did not show any attachment and proliferation in serum free media, compared to cells supplemented with 10% serum, where the doubling time was between 48 and 60h. Fibroblasts never became the prominent cell type in these cultures at any given time point. The epithelial cells reacted with antibodies to keratin AE1/AE3, keratin 14 and to involucrin, the differentiating marker.
Mhaisalkar
Priyadarshini
P
Biomedical Engineering Research Centre, Nanyang Technological University, Singapore 639798, Singapore.
Sin
Chian Kerm
CK
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division; D002470:Cell Survival; D016895:Culture Media, Serum-Free; D004847:Epithelial Cells / Q000166:cytology*; D004947:Esophagus / Q000166:cytology*; D007150:Immunohistochemistry; D007700:Kinetics; D013552:Swine
D016895:Culture Media, Serum-Free