Bioactive components of the hop strobilus: comparison of different extraction methods by capillary electrophoretic and chromatographic methods.
In the present study, the composition of the hop strobilus extract by using different extraction methods under different solvent conditions was analysed and compared. Several separation methods were applied to obtaining detailed information about the hop extract: capillary zone electrophoresis (CZE), high-performance liquid chromatography-mass-spectrometry (HPLC-MS/MS) and gas chromatography-mass-spectrometry (GC-MS). The electropherograms of different extracts varied dramatically. The oxidation reaction of the hop strobilus extract was examined.
Helmja
Kati
K
Department of Chemistry, Faculty of Science, Tallinn University of Technology, Tallinn, Estonia. helmja@staff.ttu.ee <helmja@staff.ttu.ee>
Vaher
Merike
M
Püssa
Tõnu
T
Kamsol
Kristjan
K
Orav
Anne
A
Kaljurand
Mihkel
M
D000975:Antioxidants / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods; D005419:Flavonoids / Q000302:isolation & purification*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D027582:Humulus / Q000737:chemistry*; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000737:chemistry*; D052616:Solid Phase Extraction
D000975:Antioxidants; D005419:Flavonoids; D010936:Plant Extracts