[Features of the formation of self-fertile euploid lines (2n = 42) by self-pollination of the 46-chromosome barley-wheat BC1 hybrid Hordeum marinum subsp. gussoneanum Hudson (= H. geniculatum All.) (2n = 28) x Triticum aestivum L. (2n = 42)].
We studied some features of the development of self-fertile 42-chromosome lines on the base of self-pollination progeny of 46-chromosome plants obtained by backcrossing of barley--wheat hybrids Hordeum marinum subsp. gussoneanum Hudson (= H. geniculatum All.) (2n = 28) x Triticum aestivum L. (2n = 42). The stabilization of karyotypes, resulting in 42-chromosome plants of the wheat type was generally completed by generation BC1F10. The plants of all self-pollination progenies, including BC1F10, showed some phenotypic traits characteristic of wild barley. Plants of BC1F10 with the chromosome sets 2n = 42 and 2n = 42 + t were analyzed by RAPD with a set of 115 primers. Fragments of the wild barley genome were detected in RAPD patterns with 19 primers. Cross-hybridization confirmed that these fragments belonged to the wild barley genome. We raised four phenotypically different 42-chromosome lines from grains obtained from plants of generation BC1F10, and these lines proved to be cytogenetically stable and self-fertile when grown in the field.
Pershina
L A
LA
Trubacheeva
N V
NV
Rakovtseva
T S
TS
Belova
L I
LI
Deviatkina
E P
EP
Kravtsova
L A
LA
D002678:Chimera / Q000033:anatomy & histology / Q000235:genetics / Q000502:physiology*; D032461:Chromosomes, Plant / Q000235:genetics; D003433:Crosses, Genetic; D018744:DNA, Plant / Q000032:analysis; D005298:Fertility / Q000235:genetics; D001467:Hordeum / Q000033:anatomy & histology / Q000235:genetics*; D011058:Pollen; D019105:Random Amplified Polymorphic DNA Technique; D012639:Seeds / Q000033:anatomy & histology; D014908:Triticum / Q000033:anatomy & histology / Q000235:genetics*
D018744:DNA, Plant