title: Lipoteichoic acid from Lactobacillus plantarum elicits both the production of interleukin-23p19 and suppression of pathogen-mediated interleukin-10 in THP-1 cells.
abstract: In this study, the stimulatory effects of different lactic acid bacteria strains, and their subcellular fractions, on the THP-1 cell line were evaluated. Lactobacillus plantarum was found in particular to induce high levels of IL-23p19 mRNA, but it moderately induced TNF-alpha production. IL-10 production was not entirely affected by L. plantarum stimulation. When subcellular fractions of L. plantarum were used to treat THP-1 cells, IL-23p19 mRNA expression was enhanced in a dose-responsive manner, specifically by lipoteichoic acid (LTA). The cotreatment of THP-1 cells by both L. plantarum and Staphylococcus aureus LTA resulted in decreased IL-10 production when compared with cells treated by S. aureus LTA alone. Taken together, these data suggest that LTA isolated from L. plantarum elicits stimulatory effects upon the expression of IL-23p19 and inhibitory effects on pathogen-mediated IL-10 production.
authors.affiliation: School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Suwon, Korea.
authors.forename: Han Geun
authors.initials: HG
authors.lastname: Kim
authors.forename: Min Geun
authors.initials: MG
authors.lastname: Gim
authors.forename: Joo Yun
authors.initials: JY
authors.lastname: Kim
authors.forename: Hyun Jin
authors.initials: HJ
authors.lastname: Hwang
authors.forename: Min Seok
authors.initials: MS
authors.lastname: Ham
authors.forename: Jung Min
authors.initials: JM
authors.lastname: Lee
authors.forename: Thomas
authors.initials: T
authors.lastname: Hartung
authors.forename: Jung Woo
authors.initials: JW
authors.lastname: Park
authors.forename: Seung Hyun
authors.initials: SH
authors.lastname: Han
authors.forename: Dae Kyun
authors.initials: DK
authors.lastname: Chung
mesh_terms: D002460:Cell Line; D004306:Dose-Response Relationship, Immunologic; D015870:Gene Expression; D006801:Humans; D016753:Interleukin-10 / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D053760:Interleukin-23 Subunit p19 / Q000096:biosynthesis*; D048191:Lactiplantibacillus plantarum / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology*; D009000:Monocytes / Q000382:microbiology*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D013211:Staphylococcus aureus / Q000276:immunology; D013682:Teichoic Acids / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D053760:Interleukin-23 Subunit p19; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D013682:Teichoic Acids; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; C009900:lipoteichoic acid