Central domain of a potyvirus VPg is involved in the interaction with the host translation initiation factor eIF4E and the viral protein HcPro.
Using recombinant proteins produced in bacteria or in infected plants, interactions between the VPg and HcPro of Lettuce mosaic potyvirus (LMV) and between LMV VPg and the lettuce translation initiation factor 4E, the cap-binding protein (eIF4E), were demonstrated in vitro. Interaction with eIF4E and HcPro both involved the same VPg central domain. The structure of this domain in the VPg context was predicted to include an amphiphilic alpha-helix, with the amino acids related to biological functions in various potyviruses exposed at the hydrophilic side.
Roudet-Tavert
G
G
IPV, UMR GDPP INRA-Bordeaux 2, IBVM, BP 81, F-33883 Villenave d'Ornon Cedex, France.
Michon
T
T
IPV, UMR GDPP INRA-Bordeaux 2, IBVM, BP 81, F-33883 Villenave d'Ornon Cedex, France.
Walter
J
J
IPV, UMR GDPP INRA-Bordeaux 2, IBVM, BP 81, F-33883 Villenave d'Ornon Cedex, France.
Delaunay
T
T
IPV, UMR GDPP INRA-Bordeaux 2, IBVM, BP 81, F-33883 Villenave d'Ornon Cedex, France.
Redondo
E
E
IPV, UMR GDPP INRA-Bordeaux 2, IBVM, BP 81, F-33883 Villenave d'Ornon Cedex, France.
Le Gall
O
O
IPV, UMR GDPP INRA-Bordeaux 2, IBVM, BP 81, F-33883 Villenave d'Ornon Cedex, France.
D039561:Eukaryotic Initiation Factor-4E / Q000378:metabolism*; D018545:Lactuca / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism; D017800:Potyvirus / Q000502:physiology*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary / Q000502:physiology; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D039561:Eukaryotic Initiation Factor-4E; D010940:Plant Proteins; D011994:Recombinant Proteins; D014764:Viral Proteins