Induced parthenogenesis in mandarin for haploid production: induction procedures and genetic analysis of plantlets.
This study focused on haploid induction in mandarin through in situ gynogenesis by pollination with irradiated pollen of 'Meyer' lemon. Pollination was carried out for three genotypes of mandarin with four levels of gamma-ray-irradiated pollen (150, 300, 600, and 900 Gy). The resulting seeds were characterised by a small size. Embryos were rescued in vitro and the ploidy level of the plantlets was determined by flow cytometry analysis. Haploid, diploid, triploid plantlets were obtained. The haploid parthenogenetic origin was confirmed using microsatellite marker analysis and chromosome count. Diploid and triploid plants were the result of crosses between mandarin and lemon. The induction of gynogenetic haploids of 'Fortune' (Citrus clementina Hort ex Tan. x Citrus tangerina Hort ex Tan.) and 'Ellendale' (Citrus reticulata Blanco x Citrus sinensis L. Osb) is reported here for the first time.
Froelicher
Yann
Y
Unité de Recherche Multiplication végétative, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), San Giuliano 20230, France. froelicher@cirad.fr
Bassene
Jean-Baptiste
JB
Jedidi-Neji
Emna
E
Dambier
Dominique
D
Morillon
Raphael
R
Bernardini
Geneviève
G
Costantino
Gilles
G
Ollitrault
Patrick
P
D002957:Citrus / Q000235:genetics* / Q000254:growth & development*; D004307:Dose-Response Relationship, Radiation; D005638:Fruit / Q000254:growth & development; D018525:Germination; D006238:Haploidy; D010312:Parthenogenesis / Q000235:genetics*; D011058:Pollen / Q000528:radiation effects; D012639:Seeds / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects