Imaging receptor microdomains on leukocyte subsets in live mice.
We present a simple method to identify the recruitment of leukocyte subsets and determine concurrent surface-receptor clustering in live mice. We show that CD45+ F4/80- Gr-1+ neutrophils are robustly recruited in surgery-activated cremasteric venules, whereas adherent CD45+ B220+ B lymphocytes were dominant in bone marrow venules. Most adherent Gr-1+ leukocytes are not firmly stationary but actively migrate on TNF-alpha-activated cremasteric venular endothelium and exhibit marked polarization of surface PSGL-1, but not LFA-1, to the trailing edge.
Chiang
Elaine Y
EY
Department of Medicine and Immunobiology Center, Mount Sinai School of Medicine, One Gustave L. Levy Place, New York, New York 10029, USA.
Hidalgo
Andrés
A
Chang
Jungshan
J
Frenette
Paul S
PS
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology*; D016231:Fluorescence Polarization Immunoassay / Q000379:methods*; D007962:Leukocytes / Q000166:cytology* / Q000276:immunology*; D021962:Membrane Microdomains / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*
D000954:Antigens, Surface