Linear motifs in the C-terminus of D. melanogaster cryptochrome.
The C-terminus of cryptochrome (CRY) regulates light responses in Drosophila. These include the light-dependent binding of Drosophila dCRY to the clock proteins PERIOD and TIMELESS in a yeast two-hybrid system, which we proved to be a convenient and reliable readout of the behavior of dCRY in vivo. In this study, we present a combination of in silico analysis and experimental validation in yeast, to identify novel functional motifs in the C-terminal region of dCRY. Our results suggest that linear motifs are present in this small region, which is a likely hotspot for molecular interactions.
Hemsley
Matthew J
MJ
Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, UK.
Mazzotta
Gabriella M
GM
Mason
Moyra
M
Dissel
Stephane
S
Toppo
Stefano
S
Pagano
Mario A
MA
Sandrelli
Federica
F
Meggio
Flavio
F
Rosato
Ezio
E
Costa
Rodolfo
R
Tosatto
Silvio C E
SC
D000818:Animals; D056931:Cryptochromes; D004331:Drosophila melanogaster; D015180:Electrophoresis, Gel, Two-Dimensional; D005420:Flavoproteins / Q000737:chemistry* / Q000235:genetics; D047468:Immunoprecipitation; D009154:Mutation; D010766:Phosphorylation; D011487:Protein Conformation; D020798:Two-Hybrid System Techniques
D056931:Cryptochromes; D005420:Flavoproteins