title: Enzymatic transformation of biologically active 1,3;1,6-beta-D-glucan. Structure and activity of resulting fragments.
abstract: The fragmentation of the biologically active 1,3;1,6-beta-D-glucan Antivir by endo-1,3-beta-D-glucanase LIV from crystalline styles of the marine mollusk Spisula sachalinensis was carried out. It was found that low molecular mass oligomers possessing a stabilizing effect on membranes and anti-viral activity against tobacco mosaic virus appeared in the process of enzymatic hydrolysis of Antivir. Biological activity of 1,3;1,6-beta-D-glucooligo- and polysaccharides was found to be associated with molecular mass (polymerization degree (n) not less than 14) and with presence of intralinked beta-1,6-connected monosaccharide residues. Probably, decrease in molecular mass is compensated by increase in number of intralinked beta-1,6-connected monosaccharide residues.
authors.affiliation: Pacific Institute of Bioorganic Chemistry, Far East Division, Russian Academy of Sciences, Vladivostok, 690022, Russia.
authors.forename: L A
authors.initials: LA
authors.lastname: Elyakova
authors.forename: V V
authors.initials: VV
authors.lastname: Isakov
authors.forename: L A
authors.initials: LA
authors.lastname: Lapshina
authors.forename: V P
authors.initials: VP
authors.lastname: Nagorskaya
authors.forename: G N
authors.initials: GN
authors.lastname: Likhatskaya
authors.forename: T N
authors.initials: TN
authors.lastname: Zvyagintseva
authors.forename: A V
authors.initials: AV
authors.lastname: Reunov
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry; D043326:Glucan 1,3-beta-Glucosidase / Q000378:metabolism*; D005936:Glucans / Q000737:chemistry* / Q000378:metabolism; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000737:chemistry; D049890:Spisula / Q000201:enzymology*; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
chemical_list: D000998:Antiviral Agents; D005936:Glucans; D009844:Oligosaccharides; C033671:epiglucan; D043326:Glucan 1,3-beta-Glucosidase