Histomorphometry and immunohistochemical features of grade I (WHO) oral radiomucositis.
OBJECTIVE
The aims of this study were to describe the immunohistopathological and morphometric features of oral mucositis grade I (WHO).


METHODS
Ten samples of oral mucositis were biopsied and submitted to histopathological, morphometric and immunohistochemical analyses (CD68, Ki-67 and p53). The samples were compared with the buccal mucosa of head and neck cancer patients before radiotherapy (NMCP), normal buccal mucosa (NM) and oral dysplasia (OD).


RESULTS
Epithelial thickness, area and perimeter were decreased in oral mucositis and inflammatory components, increased when compared with NMCP. CD68 immunoreactivity, near to the epithelium, was more evident in oral mucositis than in NMCP (P = 0.01). The Ki-67 counts were higher in oral mucositis than in NM and NMCP (P = 0.001 and P = 0.043, respectively), but without any difference with OD (P = 0.284). The p53 staining was present in all cases of mucositis and oral dysplasia, but negative in NMCP and NM.


CONCLUSIONS
Oral mucositis grade I (WHO) presented epithelial atypia and atrophy, increased inflammatory response, with relevant Ki-67 count and positiveness for p53.
Bonan
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PR
Oral Diagnosis, Dentistry School, State University of Montes Claros, UNIMONTES, Minas Gerais, Brazil. pbonan@yahoo.com
Kaminagakura
E
E
Pires
F R
FR
Vargas
P A
PA
de Almeida
O P
OP
D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D001284:Atrophy; D001706:Biopsy; D004848:Epithelium / Q000473:pathology / Q000528:radiation effects; D005260:Female; D006258:Head and Neck Neoplasms / Q000532:radiotherapy*; D006651:Histocytochemistry; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis; D008264:Macrophages / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000473:pathology / Q000528:radiation effects; D011832:Radiation Injuries / Q000209:etiology / Q000473:pathology*; D013280:Stomatitis / Q000209:etiology / Q000473:pathology*; D016159:Tumor Suppressor Protein p53 / Q000032:analysis; D000097382:CD68 Molecule
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D019394:Ki-67 Antigen; D016159:Tumor Suppressor Protein p53; C067980:CD68 antigen, human; D000097382:CD68 Molecule