[Effect of verapamil on the proliferation of normal gingival fibroblast].
OBJECTIVE
To evaluate the effect of verapamil on the proliferation of normal gingival fibroblast (NGF) in vitro.


METHODS
NGF was isolated and cultured. The 5th passage of NGF was incubated with 0, 0.1, 1, 10, 100 micromol/L verapamil respectively. Methyl thiazolyl tetrazolium (MTT) assay and flow cytometry were used to detect cell proliferation and cell cycles.


RESULTS
Incubated with 100 micromol/L verapamil for 66 h, the A value of normal gingival fibroblast was significantly lower than those without verapamil groups (P < 0.01). Incubated with 100 micromol/L verapamil for 18 h, 69% of cells were at the G(0) - G(1) phase, 27% were at the S phase. For control group (without verapamil) 41% of cells were at G(0) - G(1) phase and 49% cells were at S phase. There was significant difference between the two groups (P < 0.001).


CONCLUSIONS
100 micromol/L verapamil inhibited proliferation of normal gingival fibroblast by a cell-cycle arrest.
Luan
Qing-xian
QX
Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China. luanqx@yahoo.com.cn
Cao
Cai-fang
CF
D002121:Calcium Channel Blockers / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects*; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D014700:Verapamil / Q000494:pharmacology*
D002121:Calcium Channel Blockers; D014700:Verapamil