Identification of lanthionine synthase C-like protein-1 as a prominent glutathione binding protein expressed in the mammalian central nervous system.
Proteomic experiments were performed to identify novel glutathione (GSH) binding proteins expressed in the mammalian central nervous system. Bovine brain lysate was affinity purified using an immobilized glutathione-Sepharose column. Proteins that bound the immobilized glutathione were eluted with free glutathione and identified by one- and two-dimensional electrophoresis coupled with mass spectrometric analysis of tryptic fragments. Major proteins purified by this technique were glutathione S-transferase-mu (GST-mu) and GST-pi and lanthionine synthase C-like protein-1 (LanCL1). LanCL1 is a mammalian homologue of a prokaryotic enzyme responsible for the synthesis of thioether (lanthionine) cross-links within nascent polypeptide chains, yielding macrocyclic proteins with potent microbicidal activity. An antibody against LanCL1 was generated and applied to immunochemical studies of spinal cord tissue from SOD1G93A transgenic mice, a model for amyotrophic lateral sclerosis (ALS), wherein LanCL1 expression was found to be increased at presymptomatic stages of the disease. These results indicate LanCL1 is a glutathione binding protein possibly significant to neurodegenerative disease.
Chung
Charlotte H Y
CH
Oklahoma Medical Research Foundation, University of Oklahoma Health Science Center, Oklahoma City, Oklahoma 73118, USA.
Kurien
Biji T
BT
Mehta
Padmaja
P
Mhatre
Molina
M
Mou
Shenyun
S
Pye
Quentin N
QN
Stewart
Charles
C
West
Melinda
M
Williamson
Kelly S
KS
Post
Jan
J
Liu
Lucy
L
Wang
Rachel
R
Hensley
Kenneth
K
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D005978:Glutathione / Q000378:metabolism*; D051549:Glutathione S-Transferase pi / Q000302:isolation & purification; D005982:Glutathione Transferase / Q000302:isolation & purification; D006836:Hydro-Lyases / Q000302:isolation & purification*; D051379:Mice; D008822:Mice, Transgenic; D009097:Multienzyme Complexes / Q000302:isolation & purification*; D011485:Protein Binding; D040901:Proteomics / Q000379:methods; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
C485882:Lancl1 protein, mouse; D009097:Multienzyme Complexes; D043562:Receptors, G-Protein-Coupled; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; D006836:Hydro-Lyases; C099654:lanthionine synthase; D005978:Glutathione