In vitro sildenafil citrate use as a sperm motility stimulant.
The effect of different concentrations of sildenafil citrate (Viagra) solution in vitro on human sperm motility was assessed in 85 asthenozoospermic semen specimens. Semen samples were divided equally into six tubes, one as a control and to the others, sildenafil dissolved solution was added (v/v) in vitro with five different concentrations (4.0, 2.0, 1.0, 0.5, 0.1 mg/mL). The tubes were incubated and were followed up for sperm motility changes at 0.5, 1, 2, and 3 hours. It is demonstrated that the in vitro use of sildenafil citrate solution has a concentration-related stimulatory effect on ejaculated sperm motility.
Mostafa
Taymour
T
Andrology & Sexology Department, Faculty of Medicine, Cairo University, Cairo, Egypt. taymour1155@link.net
D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000037:antagonists & inhibitors; D000293:Adolescent; D000328:Adult; D053627:Asthenozoospermia / Q000503:physiopathology; D054706:Cyclic Nucleotide Phosphodiesterases, Type 5; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D010879:Piperazines / Q000494:pharmacology*; D011687:Purines / Q000494:pharmacology; D000068677:Sildenafil Citrate; D013081:Sperm Motility / Q000187:drug effects*; D013450:Sulfones / Q000494:pharmacology*
D010879:Piperazines; D011687:Purines; D013450:Sulfones; D000068677:Sildenafil Citrate; D015106:3',5'-Cyclic-GMP Phosphodiesterases; D054706:Cyclic Nucleotide Phosphodiesterases, Type 5; C517381:PDE5A protein, human