title: A thaumatin-like antifungal protein from the emperor banana.
abstract: A 20-kDa protein with substantial N-terminal sequence homology to thaumatin-like proteins was isolated from ripe fruits of the emperor banana, Musa basjoo cv. 'emperor banana'. The isolation procedure entailed (NH(4))(2)SO(4) precipitation, ion exchange chromatography on DEAE-cellulose, and affinity chromatography on Affi-gel blue gel. The thaumatin-like protein inhibited mycelial growth in Fusarium oxysporum and Mycosphaerella arachidicola. However, it did not affect the mitogenic response of murine splenocytes or [methyl-(3)H] thymidine incorporation by tumor cells. The activity of HIV-1 reverse transcriptase was slightly inhibited.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
authors.forename: Vincent S M
authors.initials: VS
authors.lastname: Ho
authors.forename: Jack H
authors.initials: JH
authors.lastname: Wong
authors.forename: T B
authors.initials: TB
authors.lastname: Ng
mesh_terms: D000595:Amino Acid Sequence; D000645:Ammonium Sulfate / Q000737:chemistry; D000818:Animals; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001203:Ascomycota / Q000187:drug effects; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005670:Fusarium / Q000187:drug effects; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors / Q000378:metabolism; D020128:Inhibitory Concentration 50; D051379:Mice; D008810:Mice, Inbred C57BL; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D028521:Musa / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D016415:Sequence Alignment; D013154:Spleen / Q000166:cytology / Q000187:drug effects
chemical_list: D000935:Antifungal Agents; D010940:Plant Proteins; C003427:thaumatin protein, plant; D054303:HIV Reverse Transcriptase; D000645:Ammonium Sulfate