title: Cytoskeleton dynamics: fluctuations within the network.
abstract: Out-of-equilibrium systems, such as the dynamics of a living cytoskeleton (CSK), are inherently noisy with fluctuations arising from the stochastic nature of the underlying biochemical and molecular events. Recently, such fluctuations within the cell were characterized by observing spontaneous nano-scale motions of an RGD-coated microbead bound to the cell surface [Bursac et al., Nat. Mater. 4 (2005) 557-561]. While these reported anomalous bead motions represent a molecular level reorganization (remodeling) of microstructures in contact with the bead, a precise nature of these cytoskeletal constituents and forces that drive their remodeling dynamics are largely unclear. Here, we focused upon spontaneous motions of an RGD-coated bead and, in particular, assessed to what extent these motions are attributable to (i) bulk cell movement (cell crawling), (ii) dynamics of focal adhesions, (iii) dynamics of lipid membrane, and/or (iv) dynamics of the underlying actin CSK driven by myosin motors.
authors.affiliation: Molecular and Integrative Physiological Sciences, Department of Environmental Health, Harvard School of Public Health, Boston, MA 02115, USA.
authors.forename: Predrag
authors.initials: P
authors.lastname: Bursac
authors.forename: Ben
authors.initials: B
authors.lastname: Fabry
authors.forename: Xavier
authors.initials: X
authors.lastname: Trepat
authors.forename: Guillaume
authors.initials: G
authors.lastname: Lenormand
authors.forename: James P
authors.initials: JP
authors.lastname: Butler
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Jeffrey J
authors.initials: JJ
authors.lastname: Fredberg
authors.forename: Steven S
authors.initials: SS
authors.lastname: An
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002465:Cell Movement; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D009130:Muscle, Smooth / Q000648:ultrastructure*; D009218:Myosins / Q000378:metabolism; D051381:Rats
chemical_list: D000199:Actins; D008024:Ligands; D009218:Myosins