The behavior of neural stem cells on biodegradable synthetic polymers.
The biocompatibility of polymer scaffolds as neural stem cell transplantation matrices has not yet been studied extensively. In this study, we evaluated the biocompatibility of various biodegradable polymers for neural stem cells. The biocompatibility tests were performed by culturing hippocampal progenitor cells (HiB5) on films of poly(lactic-co-glycolic acid) (PLGA), poly(L-lactide-co-epsilon-caprolactone) (PLCL) and poly(L-lactic acid) (PLLA) or in the presence of extracts from these polymers. Specifically, the viability, mitochondrial metabolic activity, proliferation, apoptosis and neurite out-growth of HiB5 cells were examined in biocompatibility tests. Among the tested polymers, PLGA performed best with respect to cell viability, mitochondrial metabolic activity and apoptotic activity. Compared to the other polymers, PLLA showed the worst results in all categories evaluated. PLGA also showed favorable results for neurite out-growth of HiB5 cells. The results of this study demonstrate the promising biocompatibility of PLGA as a scaffold for neural stem cell transplantation for nerve regeneration.
Bhang
Suk Ho
SH
Department of Bioengineering, Hanyang University, Seoul 133-791, South Korea.
Lim
Jung Su
JS
Choi
Cha Yong
CY
Kwon
Yunhee Kim
YK
Kim
Byung-Soo
BS
D000818:Animals; D017209:Apoptosis; D001672:Biocompatible Materials; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D006624:Hippocampus / Q000166:cytology; D019344:Lactic Acid; D008422:Materials Testing; D009416:Nerve Regeneration; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D011091:Polyesters; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D051381:Rats; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D023822:Tissue Engineering
D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C033616:poly(lactide)