Involvement of oxidative stress in seizures induced by diphenyl diselenide in rat pups.
In the present study the potential neurotoxicity of diphenyl diselenide, as measured by the manifestation of seizures in rat pups (postnatal days, PND, 12-14) was evaluated. The results suggest that the latency for the appearance of tonic-clonic seizures, characterized by rearing and falling of rat pups body, was dependent of the dose tested. Diphenyl diselenide at high doses induced seizure episodes in rat pups. The highest dose of diphenyl diselenide (500 mg/kg) increased the levels of lipid peroxidation and catalase activity as well as decreased delta-ALA-D (delta-aminolevulinate dehydratase) and Na(+), K(+) ATPase activity in the brain of rat pups. Our results indicate the possible involvement of free radical oxygen injury in diphenyl diselenide-induced seizures. The data obtained with the dose of 150 mg/kg in the brain of rats that exhibited seizures are: an increase in lipid peroxidation levels; the lack of effect on catalase activity; an inhibition of delta-ALA-D activity, supporting that the enzyme activity is more sensitive than other parameters analyzed as an indicator of oxidative stress. The lowest dose of diphenyl diselenide emphasizes the relationship between the appearance of seizures and the latency for the onset of the first episode. Taken together, this paper could add to our understanding of diphenyl diselenide neurotoxic effect demonstrated by the appearance of seizures which are, at least in part, related to the oxidative stress.
Prigol
Marina
M
Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, CEP 97105-900, RS, Brazil.
Wilhelm
Ethel A
EA
Schneider
Caroline C
CC
Rocha
Joao B T
JB
Nogueira
Cristina W
CW
Zeni
Gilson
G
D000284:Administration, Oral; D000367:Age Factors; D000704:Analysis of Variance; D000818:Animals; D001555:Benzene Derivatives / Q000008:administration & dosage / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology; D009498:Neurotoxins / Q000008:administration & dosage / Q000633:toxicity*; D016566:Organoselenium Compounds / Q000008:administration & dosage / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D000623:Porphobilinogen Synthase / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000139:chemically induced* / Q000378:metabolism; D016037:Single-Blind Method; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects* / Q000378:metabolism
D001555:Benzene Derivatives; D009498:Neurotoxins; D016566:Organoselenium Compounds; C061132:diphenyldiselenide; D000623:Porphobilinogen Synthase; D000254:Sodium-Potassium-Exchanging ATPase