title: Effect of leptin on prolactin and insulin-like growth factor-I secretion by cultured rat endometrial stromal cells.
abstract: OBJECTIVE
  To study the possible effect of leptin on PRL and insulin-like growth factor (IGF)-I secretion from rat endometrial stromal cells.
  
  
  METHODS
  The effect of recombinant murine leptin on the secretion of PRL and IGF-I by cultured rat endometrial cells was investigated.
  
  
  METHODS
  Academic institutions.
  
  
  METHODS
  Laboratory bred virgin female rats aged 3-4 months.
  
  
  METHODS
  Endometrial stromal cell (ESC) cultures in the fourth passage stimulated with 1-1,000 ng/mL of leptin for 24 hours and with 1 ng/mL leptin for 24-72 hours.
  
  
  METHODS
  Measurement of PRL and IGF-I levels in the conditioned media by enzyme immunoassay.
  
  
  RESULTS
  Endometrial stromal cells grown in vitro secreted both PRL and IGF-I into the medium and the concentrations significantly increased with passage of time even in the absence of leptin. The increase in PRL was seen mainly at 72 hours and in IGF-I at 24 and 72 hours. Presence of leptin in the culture medium (1-1,000 ng/mL) further enhanced PRL secretion in a dose-dependent manner and this effect was seen with all leptin doses used. Leptin also increased PRL secretion in a time-dependent manner and the increase was seen at 24, 48, and 72 hours. Leptin did not significantly affect IGF-I secretion either in a dose- or a time-dependent manner.
  
  
  CONCLUSIONS
  Biological effects of leptin on the rat endometrium include dose- and time-dependent stimulatory effects on stromal cell PRL secretion.
authors.affiliation: Department of Physiology, University of Colombo, Colombo, Sri Lanka. khtennekoon@med.cmb.ac.lk
authors.forename: Kamani H
authors.initials: KH
authors.lastname: Tennekoon
authors.forename: Thampoe
authors.initials: T
authors.lastname: Eswaramohan
authors.forename: Eric H
authors.initials: EH
authors.lastname: Karunanayake
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D020738:Leptin / Q000494:pharmacology*; D011388:Prolactin / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology; D017154:Stromal Cells / Q000187:drug effects / Q000378:metabolism*
chemical_list: D020738:Leptin; D011994:Recombinant Proteins; D007334:Insulin-Like Growth Factor I; D011388:Prolactin