Analysis of slow depolarizing potential in frog taste cell induced by parasympathetic efferent stimulation under hypoxia.
Strong electrical stimulation (ES) of the frog glossopharyngeal (GP) efferent nerve induced slow depolarizing potentials (DPs) in taste cells under hypoxia. This study aimed to elucidate whether the slow DPs were postsynaptically induced in taste cells. After a block of parasympathetic nerve (PSN) ganglia by tubocurarine, ES of GP nerve never induced slow DPs in the taste cells, so slow DPs were induced by PSN. When Ca(2+) in the blood plasma under hypoxia was decreased to approximately 0.5 mM, the slow DPs reduced in amplitude and lengthened in latency. Increasing the normal Ca(2+) to approximately 20 mM increased the amplitude of slow DPs and shortened the latency. Addition of Cd(2+) to the plasma greatly reduced the amplitude of slow DPs and lengthened the latency. These data suggest that the slow DPs depend on Ca(2+) and Cd(2+) concentration at the presynaptic PSN terminals of taste disk. Antagonists, [D-Arg(1), D-Trp(7,9), Leu(11)]-substance P and L-703 606, of neurotransmitter substance P neurokinin(1) receptor completely blocked the slow DPs. Intravenous application of substance P induced a DP of approximately 7 mV and a reduction of membrane resistance of approximately 48% in taste cells. A nonselective cation channel antagonist, flufenamic acid, completely blocked the slow DPs. These findings suggest that the slow DPs are postsynaptically initiated in frog taste cells under hypoxia by opening nonselective cation channels on the postsynaptic membrane after substance P is probably released from the presynaptic PSN axon terminals.
Sato
Toshihide
T
Division of Integrated Sensory Physiology, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki 852-8588, Japan. toshi@net.nagasaki-u.ac.jp
Nishishita
Kazushisa
K
Okada
Yukio
Y
Toda
Kazuo
K
D000818:Animals; D002118:Calcium / Q000378:metabolism; D015687:Cell Hypoxia; D004525:Efferent Pathways; D005439:Flufenamic Acid / Q000494:pharmacology; D008564:Membrane Potentials; D011892:Rana catesbeiana; D013373:Substance P / Q000494:pharmacology; D013650:Taste Buds / Q000187:drug effects / Q000502:physiology*
D013373:Substance P; D005439:Flufenamic Acid; D002118:Calcium