Isolation of cDNA sequences encoding the MAP kinase HOG1 and the MAP kinase kinase PBS2 genes of the fungus Alternaria tenuissima through a genetic approach.
Alternaria tenuissima is a fungus widely present in the environment and causes diseases in plants and humans in the world. In this study, we constructed an A. tenuissima cDNA expression library in a centromeric yeast vector that allows the isolation of functional cDNA sequences from this environmental and pathogenic fungus. Through a genetic approach we have isolated and functionally characterized the cDNA sequences encoding the MAP kinase (MAPK) Hog1p and the MAPK kinase Pbs2p of A. tenuissima. AtHOG1 cDNA encodes a protein of 355 amino acids, while AtPBS2 cDNA encodes a protein of 683 amino acids.
Feng
Fei
F
Laboratory of Molecular Mycology, Department of Microbial Pesticides and Molecular Design, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing, 100080, China.
Qiu
Dewen
D
Jiang
Linghuo
L
D000528:Alternaria / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D004271:DNA, Fungal / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D005800:Genes, Fungal; D020929:Mitogen-Activated Protein Kinase Kinases / Q000235:genetics* / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics; D016415:Sequence Alignment; D015398:Signal Transduction
D018076:DNA, Complementary; D004271:DNA, Fungal; D005656:Fungal Proteins; D020928:Mitogen-Activated Protein Kinases; D020929:Mitogen-Activated Protein Kinase Kinases