Williamsia serinedens sp. nov., isolated from an oil-contaminated soil.
The taxonomic status of a bacterium designated strain IMMIB SR-4(T) isolated from an oil-contaminated soil sample was characterized by using a polyphasic approach. Chemotaxonomic investigations revealed the presence of cell-wall chemotype IV, short-chain mycolic acids that co-migrated with those extracted from members of the genus Williamsia and that on pyrolysis GC produce C(16 : 0) and C(18 : 0) fatty acids, and dihydrogenated menaquinone with nine isoprene units as the predominant menaquinone. The generic assignment was confirmed by 16S rRNA gene sequence analysis. Comparative analysis of the 16S rRNA gene sequence showed that strain IMMIB SR-4(T) formed a distinct phyletic line within the genus Williamsia, displaying sequence similarities of 95.5-98.1 % with the type strains of recognized Williamsia species. Strain IMMIB SR-4(T) was distinguished from the type strains of recognized species of the genus Williamsia based on a set of phenotypic features. The genotypic and phenotypic data indicated that strain IMMIB SR-4(T) represents a novel species of the genus Williamsia, for which the name Williamsia serinedens sp. nov. is proposed. The type strain is IMMIB SR-4(T) (=DSM 45037(T)=CCUG 53151(T)).
Yassin
A F
AF
Institut für Medizinische Mikrobiologie und Immunologie der Universität Bonn, 53127 Bonn, Germany.
Young
Chiu Chung
CC
College of Agriculture and Natural Resources, Department of Soil & Environmental Sciences, National Chung Hsing University, Taichung 402, Taiwan, ROC.
Lai
Wei-An
WA
College of Agriculture and Natural Resources, Department of Soil & Environmental Sciences, National Chung Hsing University, Taichung 402, Taiwan, ROC.
Hupfer
H
H
Kekulé-Institut für Organische Chemie und Biochemie der Universität Bonn, 53121 Bonn, Germany.
Arun
A B
AB
College of Agriculture and Natural Resources, Department of Soil & Environmental Sciences, National Chung Hsing University, Taichung 402, Taiwan, ROC.
Shen
Fo-Ting
FT
College of Agriculture and Natural Resources, Department of Soil & Environmental Sciences, National Chung Hsing University, Taichung 402, Taiwan, ROC.
Rekha
P D
PD
College of Agriculture and Natural Resources, Department of Soil & Environmental Sciences, National Chung Hsing University, Taichung 402, Taiwan, ROC.
Ho
Mann-Jing
MJ
College of Agriculture and Natural Resources, Department of Soil & Environmental Sciences, National Chung Hsing University, Taichung 402, Taiwan, ROC.
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009171:Mycolic Acids / Q000032:analysis; D010578:Petroleum; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D012989:Soil Pollutants; D013624:Taiwan
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D009171:Mycolic Acids; D010578:Petroleum; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants