title: A mariner-based transposition system for Listeria monocytogenes.
abstract: In this study, we developed a new mariner-based transposition system for Listeria monocytogenes. The mariner-based system has a high rate of transposition and a low rate of plasmid retention, and transposition is very random, making it an ideal tool for high-throughput transposon mutagenesis in L. monocytogenes.
authors.affiliation: Department of Microbiology and Immunology, VMC C5-169, Cornell University, Ithaca, NY 14853-6401, USA.
authors.forename: Min
authors.initials: M
authors.lastname: Cao
authors.forename: Alan Pavinski
authors.initials: AP
authors.lastname: Bitar
authors.forename: Hélène
authors.initials: H
authors.lastname: Marquis
mesh_terms: D004251:DNA Transposable Elements / Q000235:genetics*; D005822:Genetic Vectors; D008089:Listeria monocytogenes / Q000235:genetics*; D016254:Mutagenesis, Insertional / Q000379:methods*; D010957:Plasmids / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements