Exercise training raises expression of the cytosolic components of NADPH oxidase in rat neutrophils.
Exercise activates neutrophil burst and this effect is dependent on training status and exercise intensity. In this study, the chronic effect of treadmill exercise on phagocytosis, production of reactive oxygen metabolites and expression of NADPH oxidase components in rat neutrophils was investigated. Neutrophils were obtained by intraperitoneal lavage with PBS. After 11 weeks of training the exercised group showed increased phagocytosis capacity (49%) and production of reactive oxygen metabolites (6.6-fold) when compared with neutrophils from the sedentary group. Exercised had no effect on expression of the membrane components of NADPH oxidase (p22( phox ), gp91( phox )). In contrast, there was an increase of the p47( phox ) mRNA levels (by 126%), the cytosolic component of the enzyme. In addition, exercise increased the protein content of p47( phox ) (by 22%) and of p67( phox ) (by 2.8-fold) in neutrophils. Evidence is then presented that training to moderate exercise increases phagocytosis and production of reactive oxygen metabolites and the expression of p47( phox ) and p67( phox ) in neutrophils. Therefore, moderate exercise might enable neutrophils to respond more efficiently when exposed to pathogens.
Levada-Pires
Adriana C
AC
Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
Lambertucci
Rafael H
RH
Mohamad
Marian
M
Hirabara
Sandro M
SM
Curi
Rui
R
Pithon-Curi
Tania Cristina
TC
D000818:Animals; D003600:Cytosol / Q000201:enzymology*; D005080:Exercise Test; D015971:Gene Expression Regulation, Enzymologic; D008297:Male; D019255:NADPH Oxidases / Q000378:metabolism*; D009504:Neutrophils / Q000166:cytology* / Q000201:enzymology*; D010101:Oxygen Consumption / Q000502:physiology; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar
D019255:NADPH Oxidases