title: A Candida albicans petite mutant strain with uncoupled oxidative phosphorylation overexpresses MDR1 and has diminished susceptibility to fluconazole and voriconazole.
abstract: We showed that a Candida albicans petite mutant in which oxidative phosphorylation is uncoupled was eightfold more resistant to fluconazole and voriconazole than SC5314 but equally susceptible to ketoconazole, itraconazole, and amphotericin B. Strain P5 significantly overexpressed MDR1, which likely accounts for the decreased drug susceptibility.
authors.affiliation: University of Florida College of Medicine, JHMHC, Gainesville, FL 32610, USA.
authors.forename: Shaoji
authors.initials: S
authors.lastname: Cheng
authors.forename: Cornelius J
authors.initials: CJ
authors.lastname: Clancy
authors.forename: Katherine T
authors.initials: KT
authors.lastname: Nguyen
authors.forename: William
authors.initials: W
authors.lastname: Clapp
authors.forename: M Hong
authors.initials: MH
authors.lastname: Nguyen
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000502:physiology*; D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D025141:Drug Resistance, Fungal; D015725:Fluconazole / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D009154:Mutation; D010085:Oxidative Phosphorylation; D011743:Pyrimidines / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014230:Triazoles / Q000494:pharmacology*; D065819:Voriconazole
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000935:Antifungal Agents; D011743:Pyrimidines; D014230:Triazoles; D015725:Fluconazole; D065819:Voriconazole