title: Dientamoeba fragilis in swine population: a preliminary investigation.
abstract: Dientamoeba fragilis, a protozoan with worldwide distribution is considered to be responsible for enteric disease in humans. A wide spectrum of clinical symptoms including; diarrhoea (acute or prolonged), flatulence, abdominal pains and other unspecific bowel symptoms have been ascribed to this parasite. Asymptomatic infection has also been reported. Dientamoeba fragilis is as its name indicates an extremely delicate protozoon and only the trophozoite has ever been demonstrated in stool samples. The definitive diagnosis of this infection is based on demonstration in permanently stained stool samples. In Italy examination of ova and parasite (O&P) samples are not currently performed. This protozoan is extremely difficult to cultivate but molecular techniques such as the Polymerase Chain Reaction offer promise as a means of diagnosing infection. The epidemiology of Dientamoebiasis is not clear. This paper will present preliminary results from a study looking for the parasite's presence in swine faeces. The possible role of pigs as a reservoir of infection was studied; 121 faecal samples from breeding and fattening pigs were examined using a Giemsa permanent stain. Dientamoeba fragilis was found in 53 (43.8%) of the stool samples examined.
authors.affiliation: Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Via G. Salvemini 1, 06126 Perugia, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Crotti
authors.forename: M
authors.initials: M
authors.lastname: Sensi
authors.forename: S
authors.initials: S
authors.lastname: Crotti
authors.forename: V
authors.initials: V
authors.lastname: Grelloni
authors.forename: E
authors.initials: E
authors.lastname: Manuali
mesh_terms: D000818:Animals; D004029:Dientamoeba / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology; D013552:Swine / Q000469:parasitology*