Non-optimal levels of dietary selenomethionine alter splenocyte response and modify oxidative stress markers in female mice.
Many studies evaluating the effects of selenium (Se) status on immunity utilize inorganic Se, although selenomethionine (Se-Met) has been suggested to be more bioavailable and less toxic. In the current study, we investigated the effects of dietary Se-Met on immune system function and cellular redox status in C57BL/6N female mice fed with low (0.02 ppm), sufficient (0.2 ppm, control group), or excess Se-Met (2 ppm) in the diet for 50 days. Low Se-Met intake reduced glutathione peroxidase (GPx) activity and glutathione concentration without modifying lipoperoxidation. While low Se-Met intake also reduced the number of B cells in the spleen, it increased mitogen-induced proliferation, IL-4 and IL-12 secretion when compared to the sufficient Se-Met intake group. In comparison to controls, excess Se-Met intake increased splenocyte proliferation and reduced B cell numbers, IL-4, and IL-12 secretion without affecting oxidative stress markers. These data suggest that Se-Met supplementation should be carefully evaluated as it many influence immune function.
Vega
Libia
L
Sección Externa de Toxicología, Centro de Investigación y de Estudios Avanzados, Av. IPN 2508, San Pedro Zacatenco, México, DF 07360, Mexico. lvega@cinvestav.mx
Rodríguez-Sosa
Miriam
M
García-Montalvo
Eliud A
EA
Del Razo
Luz María
LM
Elizondo
Guillermo
G
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000633:toxicity; D001402:B-Lymphocytes / Q000187:drug effects / Q000473:pathology; D015415:Biomarkers / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D004032:Diet; D004305:Dose-Response Relationship, Drug; D005260:Female; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007107:Immune System / Q000187:drug effects* / Q000473:pathology; D018664:Interleukin-12 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000187:drug effects*; D012645:Selenomethionine / Q000008:administration & dosage* / Q000633:toxicity; D013154:Spleen / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D015430:Weight Gain / Q000187:drug effects
D000975:Antioxidants; D015415:Biomarkers; D018664:Interleukin-12; D015847:Interleukin-4; D012645:Selenomethionine; D005979:Glutathione Peroxidase; D005978:Glutathione