Ion chromatography on a latex-coated silica monolith column.
A silica monolith column (Merck Chromolith, 100 mm x 4.6 mm) has been coated with Dionex AS9-SC latex nanoparticles to convert the column into an anion-exchange stationary phase. For comparison purposes, a reversed-phase silica monolith was also converted into an anion-exchange column by coating with the cationic surfactant didodecyldimethylammonium bromide (DDAB). Separations of common inorganic anions were carried out using 7.5 or 5.0 mM 4-hydroxybenzoic acid at pH 7.0 along with suppressed conductivity detection. Direct comparisons were then made between the two columns in terms of selectivity, efficiency and stability. The latex-coated column was on average 50% more efficient than the DDAB-coated column. A 10% decrease in retention times was observed on the DDAB column over 11 h of continuous eluent flow, while the latex coating exhibited <1% change in retention even after 2.5 months of periodic use.
Glenn
Karen M
KM
Department of Chemistry, University of Alberta, Gunning/Lemieux Chemistry Centre, Edmonton, AB, Canada T6G 2G2.
Lucy
Charles A
CA
Haddad
Paul R
PR
D000838:Anions / Q000737:chemistry; D002852:Chromatography, Ion Exchange / Q000295:instrumentation* / Q000379:methods*; D007840:Latex / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry*
D000838:Anions; D007840:Latex; D012822:Silicon Dioxide