Clonorchis sinensis: molecular cloning, enzymatic activity, and localization of yolk ferritin.
Ferritin is an intracellular protein that is involved in iron metabolism. A cDNA clone of Clonorchis sinensis (CsFtn), 565 bp long, encoded a putative polypeptide of 166 amino acids. CsFtn cDNA revealed a putative loop-stem structure similar to iron-responsive element (IRE). CsFtn polypeptide appeared homologous to the ferritin of trematodes with high sequential identity. Phylogenetic tree analysis showed that CsFtn clustered with the ferritins of other flukes. Recombinant CsFtn protein was produced and purified from an Escherichia coli system, and immune mouse serum was raised against CsFtn. Recombinant CsFtn showed iron-uptake ability. In adult C. sinensis, CsFtn protein was found to localize in vitelline follicles and eggs. Based on these results, CsFtn cDNA is considered to encode a C. sinensis yolk ferritin.
Tang
Yi
Y
Department of Parasitology, Chung-Ang University College of Medicine, Tongjak-gu, Seoul 156-756, Republic of Korea.
Cho
Pyo Yun
PY
Kim
Tae Im
TI
Hong
Sung-Jong
SJ
D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D001483:Base Sequence; D003004:Clonorchis sinensis / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000737:chemistry; D018509:DNA, Helminth / Q000737:chemistry; D005293:Ferritins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting; D007501:Iron / Q000378:metabolism; D035925:Iron-Regulatory Proteins / Q000737:chemistry / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D012333:RNA, Messenger / Q000737:chemistry; D011817:Rabbits; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
D000947:Antigens, Helminth; D018076:DNA, Complementary; D018509:DNA, Helminth; D007106:Immune Sera; D035925:Iron-Regulatory Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D005293:Ferritins; D007501:Iron