title: Trypanosoma cruzi: involvement of glycoinositolphospholipids in the attachment to the luminal midgut surface of Rhodnius prolixus.
abstract: Trypanosoma cruzi epimastigotes adhere in vivo to the luminal surface of their triatomid vector digestive tract by molecular mechanisms, as yet, unknown. Here, we show that the administration of 0.5 microM epimastigote major surface glycoinositolphospholipids (GIPLs) to the infected bloodmeal inhibits up to 90% parasite infection in Rhodnius prolixus. The parasite behavior was investigated in vitro using fragments of the insect midgut. The addition of GIPLs in concentration as low as 50-100 nM impaired 95% the attachment of epimastigotes. Previous treatment of GIPLs with trifluoroacetic acid to remove the terminal beta-galactofuranosyl residues reversed 50% the epimastigote in vitro attachment. The binding sites of purified GIPLs on the luminal surface of the posterior midgut were exposed by immunofluorescence microscopy. These observations indicate that GIPLs are one of the components involved in the adhesion of T. cruzi to the luminal insect midgut surface and possibly one of the determinants of parasite infection in the insect vector.
authors.affiliation: Laboratório de Biologia Celular e Tecidual, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ, Brazil.
authors.forename: Nadir F S
authors.initials: NF
authors.lastname: Nogueira
authors.forename: Marcelo S
authors.initials: MS
authors.lastname: Gonzalez
authors.forename: José Eugênio
authors.initials: JE
authors.lastname: Gomes
authors.forename: Wanderley
authors.initials: W
authors.lastname: de Souza
authors.forename: Eloi S
authors.initials: ES
authors.lastname: Garcia
authors.forename: Patricia
authors.initials: P
authors.lastname: Azambuja
authors.forename: Lilian L
authors.initials: LL
authors.lastname: Nohara
authors.forename: Igor C
authors.initials: IC
authors.lastname: Almeida
authors.forename: Bianca
authors.initials: B
authors.lastname: Zingales
authors.forename: Walter
authors.initials: W
authors.lastname: Colli
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D014355:Chagas Disease / Q000469:parasitology / Q000635:transmission; D004305:Dose-Response Relationship, Drug; D006017:Glycolipids / Q000737:chemistry / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D007303:Insect Vectors / Q000469:parasitology*; D018613:Microscopy, Confocal; D018715:Microscopy, Video; D010743:Phospholipids / Q000737:chemistry / Q000502:physiology*; D011817:Rabbits; D012239:Rhodnius / Q000469:parasitology*; D021241:Spectrometry, Mass, Electrospray Ionization; D014349:Trypanosoma cruzi / Q000737:chemistry / Q000187:drug effects / Q000502:physiology*
chemical_list: D006017:Glycolipids; D010743:Phospholipids; C115336:glycoinositolphospholipid, Trypanosoma