[Relationship between nuclear factor kappa B signal transduction way and the sensitivity of tongue carcinoma cell to Pingyangmycin chemotherapy].
OBJECTIVE
To examine the relationship between NF-kappaB signal transduction way and the sensitivity of Tca8113 carcinoma cell to Pingyangmycin chemotherapy.


METHODS
2 mg/L antisense p65 oligodeoxynucle-otides (5'-GAACAGTTCGTCCATGGCCG-3') was transfected into Tca8113 cells through Lepofectin vectin, then the cells were treated with 8 mg/L Pingyangmycin. After 3 h and 6 h, the expression of p65 in nucleus was studied with immunohistochemical methods and Western blot analyses. After 48 h, the inhibitory rate of cell growth was detected with MTT assay.


RESULTS
The NF-kappaB/p65 signal transduction way in Tca8113 cells was activated after treated with Pingyangmycin. But the transfection of antisense p65 oligodeoxynucle-otides inhibited the activation of the signal transduction, the p65 expression in nucleus was decreased significantly (P < 0.05) at 6 h, and the inhibitory rate of cell growth was increased significantly (P < 0.05) at 48 h.


CONCLUSIONS
The results suggested the chemotherapeutic sensitivity of Tca8113 carcinoma cell to Pingyangmycin was improved by the inhibition of NF-kappaB/p65 signal transduction.
Wu
Jun-Lou
JL
Dept. of Oral and Maxillofacial Surgery, Shandong Provincial Hospital, Jinan 250021, China.
Liang
Xin-Hua
XH
Mao
Zu-Yi
ZY
Xiao
Gui-Zhou
GZ
D001761:Bleomycin / Q000031:analogs & derivatives; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D016328:NF-kappa B; D015398:Signal Transduction; D014062:Tongue Neoplasms; D051996:Transcription Factor RelA; D014162:Transfection
D016328:NF-kappa B; D051996:Transcription Factor RelA; D001761:Bleomycin; C025703:bleomycetin