Salidroside production by hairy roots of Rhodiola sachalinensis obtained after transformation with Agrobacterium rhizogenes.
Hairy roots induced by Agrobacterium rhizogenes grow faster, and are considered as genetically stable. These hairy roots can be used as an interesting material for the production of secondary metabolites of pharmaceutical value. Salidroside has been identified as the major compounds from the roots of Rhodiola sachalinensis A. BOR. Here, we provide an update that adds new perspectives on the prospects and challenges of producing Salidroside from hairy roots induced by Agrobacterium rhizogene in Rhodiola sachalinensis A. BOR. For high salidroside production, the optimal concentration for precursor (Tyrosol, Tyrosine, and Phenylalanine) and elicitor (Aspergillus niger, Coriolus versicolor, and Ganoderma lucidum) was added in the LB liquid medium, respectively. The addition of elicitor in the liquid MS medium and the utilization of precursor from chemical feeding enhanced biomass accumulation and salidroside production. The optimal concentration for elicitor and precursor in the liquid medium was 0.05 mg/l and 1 mmol/l, respectively.
Zhou
Xiaofu
X
Institute of Genetics and Physiology, Jilin Normal University, Siping, Jilin 136000, China.
Wu
Yuxia
Y
Wang
Xingzhi
X
Liu
Bao
B
Xu
Hongwei
H
D000363:Agaricales / Q000502:physiology; D001234:Aspergillus niger / Q000502:physiology; D018533:Biomass; D032902:Ganoderma / Q000502:physiology; D005960:Glucosides / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism*; D010649:Phenylalanine / Q000378:metabolism; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D030821:Plants, Genetically Modified; D012231:Rhizobium / Q000235:genetics*; D029771:Rhodiola / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D014170:Transformation, Genetic; D014443:Tyrosine / Q000737:chemistry / Q000378:metabolism
D005960:Glucosides; D010636:Phenols; D010937:Plant Growth Regulators; C011867:4-hydroxyphenylethanol; D014443:Tyrosine; D010649:Phenylalanine; C009172:rhodioloside; D010626:Phenylethyl Alcohol