title: Mast cells and lymphocyte subsets in pulps from healthy and carious human teeth.
abstract: OBJECTIVE
  To evaluate the presence of cytolytic T lymphocytes (CD8+), memory T cells (CD45RO+), helper T lymphocytes (CD4+), and mast cells in pulps from healthy and carious human teeth.
  
  
  METHODS
  The teeth were separated into groups: I = unerupted; II = partially erupted, without caries; III = erupted, without caries; IV = erupted with shallow dentine caries; and V = teeth with pulp polyps. The immunoperoxidase staining procedure was used to detect CD8, CD45RO, CD4, and tryptase (mast cell marker) antigens. The number of each cell type was obtained by counting the number of cells per mm2.
  
  
  RESULTS
  Mast cells were only present in pulp polyps. Pulps from carious teeth contained more CD4+ and CD8+ cells than from noncarious teeth. There was a significant decrease in the number of lymphocytes in pulp polyps in comparison to the other groups.
  
  
  CONCLUSIONS
  Mast cells probably do not contribute to the early vascular or specific immune responses in the initial dental pulp pathosis, although they may be involved in a chronic phase of pulp inflammation such as pulp polyps. On the other hand, CD4+ and CD8+ T cells participate mainly in initial phenomena of the immune response to incipient caries and seem not to substantially contribute to the response in pulp polyps.
authors.affiliation: Department of Stomatology, Bauru Dental School, University of São Paulo, Bauru, Brazil.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Freitas
authors.forename: Carolina Purens
authors.initials: CP
authors.lastname: Novaretti
authors.forename: Camila Oliveira
authors.initials: CO
authors.lastname: Rodini
authors.forename: Aline Carvalho
authors.initials: AC
authors.lastname: Batista
authors.forename: Vanessa Soares
authors.initials: VS
authors.lastname: Lara
mesh_terms: D000293:Adolescent; D000328:Adult; D000704:Analysis of Variance; D015704:CD4 Antigens / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes; D016827:CD8 Antigens / Q000032:analysis; D018414:CD8-Positive T-Lymphocytes; D003731:Dental Caries / Q000276:immunology* / Q000473:pathology; D003782:Dental Pulp / Q000166:cytology / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D007156:Immunologic Memory; D017493:Leukocyte Common Antigens / Q000032:analysis; D018655:Lymphocyte Count; D008407:Mast Cells / Q000276:immunology*; D011671:Pulpitis / Q000276:immunology* / Q000473:pathology; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic; D053802:Tryptases / Q000032:analysis
chemical_list: D015704:CD4 Antigens; D016827:CD8 Antigens; D017493:Leukocyte Common Antigens; D053802:Tryptases