Comparative analysis of GDF 8 (myostatin) in Bos indicus and Bos taurus.
We report the myostatin gene sequence of Bos indicus cattle in comparison to Bos taurus. B. indicus genomic sequence was obtained by overlapping PCR amplification of genomic DNA. Exon splice sites were confirmed by mRNA sequencing. There were 5 exonic single nucleotide polymorphisms (SNP) only one of which was a non-synonymous mutation that resulted in a serine to asparagine (S214N) amino acid substitution. The B. indicus gene has two insertions of 16 and 12 bases in the first intron. In addition, SNPs in the 3' UTR and intronic regions are also reported.
Tantia
Madhu S
MS
National Bureau of Animal Genetic Resources, Post Box 129, Karnal 132001, Haryana, India. mstantia@nbagr.ernet.in
Vijh
Ramesh K
RK
Kumar
S T Bharani
ST
Mishra
Bina
B
Reecy
James M
JM
D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D017931:DNA Primers; D004251:DNA Transposable Elements / Q000235:genetics; D008969:Molecular Sequence Data; D055435:Myostatin; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D012333:RNA, Messenger / Q000235:genetics*; D017423:Sequence Analysis, RNA; D013045:Species Specificity; D016212:Transforming Growth Factor beta / Q000235:genetics*
D017931:DNA Primers; D004251:DNA Transposable Elements; D055435:Myostatin; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta