A new parvicapsulid (Myxosporea) species in adult pink salmon, Oncorhynchus gorbuscha, from the Quinsam River, British Columbia, Canada.
Myxospores consistent with species of Parvicapsula were observed in kidney of 15 of 95 (15.8%) adult pink salmon, Oncorhynchus gorbuscha, collected from the Quinsam River, British Columbia, Canada. The spores were elongate and curved with unequal valves, and 2 spherical-to-subspherical polar capsules within a highly refractile capsular region. The spores were unlike those of P. minibicornis found in nearby populations of Pacific salmon, Oncorhynchus spp. The spore dimensions were similar to those of Parvicapsula pseudobranchicola from Atlantic salmon, Salmo salar, in Norway, and the spores seemed similar to an undescribed Parvicapsula sp. from several Oncorhynchus spp. in Puget Sound, Washington. A sequence of 1,480 base pairs (bp) of the small subunit ribosomal RNA gene (SSU rDNA) of the parasite from pink salmon was most similar to, but distinct from, that of other Parvicapsula spp. The parasite is described as a new species, Parvicapsula kabatai n. sp. Polymerase chain reactions amplified a 158-bp sequence, unique to P. kabatai n. sp., from 22 of 93 (23.7%) adult pink salmon kidney samples, from 3 of 3 juvenile pink salmon collected in the ocean 125 km north of the Quinsam River, and from 2 of 5 archival coho salmon, Oncorhynchus kisutch, samples from Puget Sound. The parasite occurs within the lumen and epithelium of renal tubules and ducts, and within the renal interstitium. Concurrent infections with extrasporogonic stages of the myxosporean CKX, the microsporidian Loma salmonae, and a Myxidium sp. also were observed in the adult pink salmon.
Jones
Simon
S
Pacific Biological Station, 3190 Hammond Bay Road, Nanaimo, British Columbia, V9T 6N7 Canada. joness@pac.dfo-mpo.gc.ca
Prosperi-Porta
Gina
G
Dawe
Sheila
S
D000818:Animals; D001483:Base Sequence; D001955:British Columbia / Q000453:epidemiology; D003001:Cloning, Molecular; D016054:DNA, Protozoan / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D056890:Eukaryota / Q000145:classification* / Q000235:genetics / Q000648:ultrastructure; D005393:Fish Diseases / Q000453:epidemiology / Q000469:parasitology*; D007668:Kidney / Q000469:parasitology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D011529:Protozoan Infections, Animal / Q000453:epidemiology / Q000469:parasitology*; D016053:RNA, Protozoan / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D045483:Rivers; D012474:Salmon / Q000469:parasitology*; D033761:Spores, Protozoan / Q000648:ultrastructure
D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D016053:RNA, Protozoan; D012335:RNA, Ribosomal