title: Protective action of indapamide, a thiazide-like diuretic, on ischemia-induced injury and barrier dysfunction in mouse brain microvascular endothelial cells.
abstract: The aim of the present study was to elucidate the effects of indapamide on ischemic damage to the blood-brain barrier (BBB) in vitro. The ischemia/reperfusion conditions employed here significantly decreased the viability of mouse brain capillary endothelial (MBEC4) cells, an effect ameliorated by indapamide. Ischemia increased the permeability of MBEC4 cells to two cellular transport markers, sodium fluorescein and Evan's blue-albumin. Indapamide reduced the ischemia-induced hyperpermeability of cells. These results suggest that indapamide may have a protective role against ischemia-induced injury and dysfunction of the BBB.
authors.affiliation: Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Nishioku
authors.forename: Fuyuko
authors.initials: F
authors.lastname: Takata
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yamauchi
authors.forename: Noriko
authors.initials: N
authors.lastname: Sumi
authors.forename: Ikumi
authors.initials: I
authors.lastname: Yamamoto
authors.forename: Akiko
authors.initials: A
authors.lastname: Fujino
authors.forename: Mikihiko
authors.initials: M
authors.lastname: Naito
authors.forename: Takashi
authors.initials: T
authors.lastname: Tsuruo
authors.forename: Hideki
authors.initials: H
authors.lastname: Shuto
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Kataoka
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects* / Q000503:physiopathology; D001921:Brain / Q000098:blood supply* / Q000187:drug effects; D002545:Brain Ischemia / Q000188:drug therapy* / Q000503:physiopathology; D002199:Capillary Permeability / Q000187:drug effects; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D002540:Cerebral Cortex / Q000098:blood supply / Q000187:drug effects; D004396:Coloring Agents / Q000494:pharmacology; D004232:Diuretics / Q000494:pharmacology*; D042783:Endothelial Cells / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D005070:Evans Blue / Q000494:pharmacology; D019793:Fluorescein; D005456:Fluorescent Dyes; D007190:Indapamide / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D015427:Reperfusion Injury / Q000188:drug therapy / Q000503:physiopathology / Q000517:prevention & control
chemical_list: D004396:Coloring Agents; D004232:Diuretics; D005456:Fluorescent Dyes; D005070:Evans Blue; D007190:Indapamide; D019793:Fluorescein