title: BG60S dissolution interferes with osteoblast calcium signals.
abstract: We investigated the influence of extracellular calcium concentration, caused by the dissolution of a bioactive glass with 60% of silicon (BG60S), on intracellular calcium (Ca(i) (2 +)) signals and expression of inositol 1, 4, 5-triphosphate receptors (InsP(3)R) in primary culture of osteoblasts. We found that BG60S caused an increase in Ca(i) (2 +) signals in this cell type. Additionally, osteoblasts pre-incubated in the presence of BG60S showed an increase in Ca(i) (2 +) when cells were stimulated with vasopressin. On the other hand, a decrease in Ca(i) (2 +) signals were observed in osteoblasts pre-treated with BG60S and stimulated with KCl. We furher found that in osteoblasts, the type I InsP(3)R is preferentially distributed in the nucleus while the type II InsP(3)R in the cytoplasm. Preincubation of osteoblasts with BG60S altered the receptor expression level, increasing the type I InsP(3)R in the nucleus and decreasing type II InsP(3)R in the cytosol. Together, our results showed that in osteoblasts, BG60S increased Ca(i) (2 +)signals and altered Ca(i) (2 +) machinery.
authors.affiliation: Department of Physiology and Biophysics, Federal University of Minas Gerais, Av Antonio Carlos 6627, Pampulha, ICB, Bloco A4, Belo Horizonte, MG, Brazil. patricia.valerio@terra.com.br
authors.forename: P
authors.initials: P
authors.lastname: Valério
authors.forename: M M
authors.initials: MM
authors.lastname: Pereira
authors.forename: A M
authors.initials: AM
authors.lastname: Goes
authors.forename: M F
authors.initials: MF
authors.lastname: Leite
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000737:chemistry; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000502:physiology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D053496:Inositol 1,4,5-Trisphosphate Receptors / Q000378:metabolism*; D008422:Materials Testing; D010006:Osteoblasts / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012825:Silicon / Q000008:administration & dosage*
chemical_list: C520663:BG60S compound; D001672:Biocompatible Materials; D053496:Inositol 1,4,5-Trisphosphate Receptors; D002118:Calcium; D012825:Silicon