title: Controlling retinal pigment epithelium injury after experimental detachment of the retina.
abstract: OBJECTIVE
  Damage induced by detachment of the neural retina and the retinal pigment epithelium (RPE) can be reduced by dark adaptation. The authors evaluated the influence of the duration of dark adaptation, time of day, and modification of the melatonin-dopamine pathway on acute RPE lesions induced by mechanical detachment.
  
  
  METHODS
  BALB/c mice were studied at different times of day and different periods of dark adaptation. Some mice were treated with melatonin or sulpiride, a D2 dopamine receptor antagonist. Enucleated eyes and different saline solutions were used in experiments ex vivo. Retinal detachments in vivo were made by subretinal injections of hyaluronic acid. RPE cell damage was quantitatively evaluated with a dye exclusion procedure, and their viability was tested by preservation of tight junctions in culture. Lectin histochemistry was used to examine the interphotoreceptor matrix (IPM).
  
  
  RESULTS
  Significant propidium iodide (PI) incorporation in RPE cells was detected after ex vivo separation during daytime, but it was very low when detachment took place at night after 24 to 48 hours of dark adaptation. PI exclusion was achieved during daytime after a single hour of dark adaptation when mice were pretreated with melatonin or sulpiride. Reduction of RPE cell damage was accompanied by decreased lectin binding to cone sheaths.
  
  
  CONCLUSIONS
  A combination of time of day and length of dark adaptation decreased damage induced by detachment of the retina ex vivo and in vivo. Melatonin or sulpiride could replace these environmental factors. Therefore, melatonin and dopamine pathways might be involved in the control of IPM properties and retina/RPE interactions.
authors.affiliation: Facultad de Ciencias Biomédicas, Universidad Austral, Buenos Aires, Argentina.
authors.forename: María
authors.initials: M
authors.lastname: Iribarne
authors.forename: M Valeria
authors.initials: MV
authors.lastname: Canto-Soler
authors.forename: Vanesa
authors.initials: V
authors.lastname: Torbidoni
authors.forename: Angela M
authors.initials: AM
authors.lastname: Suburo
mesh_terms: D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D003623:Dark Adaptation; D065127:Dopamine D2 Receptor Antagonists; D005109:Extracellular Matrix; D006651:Histocytochemistry; D037102:Lectins / Q000378:metabolism; D008297:Male; D008550:Melatonin / Q000494:pharmacology; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D023421:Models, Animal; D010750:Phosphoproteins / Q000378:metabolism; D010857:Pigment Epithelium of Eye / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D011419:Propidium / Q000378:metabolism; D012163:Retinal Detachment / Q000473:pathology*; D013469:Sulpiride / Q000494:pharmacology; D013997:Time Factors; D062826:Zonula Occludens-1 Protein
chemical_list: D065127:Dopamine D2 Receptor Antagonists; D037102:Lectins; D008565:Membrane Proteins; D010750:Phosphoproteins; C569043:Tjp1 protein, mouse; D062826:Zonula Occludens-1 Protein; D011419:Propidium; D013469:Sulpiride; D008550:Melatonin