title: Loss and fragmentation of chromosome 5 are major events linked to the adaptation of rad52-DeltaDelta strains of Candida albicans to sorbose.
abstract: Candida albicans can adapt and grow on sorbose plates by losing one copy of Chr5. Since rad52 mutants of Saccharomyces cerevisiae lose chromosomes at a high rate, we have investigated the ability of C. albicans rad52 to adapt to sorbose. Carad52-DeltaDelta mutants generate Sou(+) strains earlier than wild-type but the final yield is lower, probably because they die at a higher rate in sorbose. As other strains of C. albicans, CAF2 and rad52-DeltaDelta derivatives generate Sou(+) strains by a loss of one copy of Chr5 about 75% of the time. In addition, rad52 strains were able to produce Sou(+) strains by a fragmentation/deletion event in one copy of Chr5, consisting of loss of a region adjacent to the right telomere. Finally, both CAF2 and rad52-DeltaDelta produced Sou(+) strains with two apparent full copies of Chr5, suggesting that additional genomic changes may also regulate adaptation to sorbose.
authors.affiliation: Departamento de Microbiología, Facultad de Ciencias, Universidad de Extremadura, 06071 Badajoz, Spain.
authors.forename: Encarnación
authors.initials: E
authors.lastname: Andaluz
authors.forename: Jonathan
authors.initials: J
authors.lastname: Gómez-Raja
authors.forename: Belén
authors.initials: B
authors.lastname: Hermosa
authors.forename: Toni
authors.initials: T
authors.lastname: Ciudad
authors.forename: E
authors.initials: E
authors.lastname: Rustchenko
authors.forename: Richard
authors.initials: R
authors.lastname: Calderone
authors.forename: Germán
authors.initials: G
authors.lastname: Larriba
mesh_terms: D000220:Adaptation, Biological / Q000235:genetics*; D002176:Candida albicans / Q000235:genetics / Q000502:physiology*; D015825:Chromosomes, Fungal / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics; D006580:Genetic Carrier Screening; D007621:Karyotyping; D020641:Polymorphism, Single Nucleotide; D017384:Sequence Deletion; D013013:Sorbose / Q000378:metabolism*
chemical_list: D004271:DNA, Fungal; D013013:Sorbose