[Inhibition activity of semenogelin and its peptides to human spermatozoa].
OBJECTIVE
To study the inhibition activity of Semenogelin (Sg) and its different peptides to human spermatozoa.


METHODS
Human Sg DNA and its N-terminal Sg and C-terminal Sg DNA were cloned into PET-100 vector. Positive colonies were screened and transformed into E. Coli BL21 (DE3). Recombinant Sg and its peptides were induced and expressed in high competent E. coli BL21 (DE3) , and purified by 50% Ni-NTA column. Inhibition activity assay was done by adding 4 different concentrations of semenogelin and its two peptides, 0, 1, 5 and 10 ng/microl, to human spermatozoa.


RESULTS
The peptide of Semenogelin that inhibits the activity of human spermatozoa was located in its N-terminal fragment. C-terminal Sg did not inhibit the activity of spermatozoa.


CONCLUSIONS
N-terminal Sg is the inhibition peptide of the whole molecular Sg. During semen liquefaction, this peptide should be cut off from the surface of human spermatozoa before they move forward.
Wang
Zeng-jun
ZJ
Department of Urology, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China. zengjunwang2002@hotmail.com
Zhang
Wei
W
Wu
Hong-fei
HF
Xu
Yuan-geng
YG
D003001:Cloning, Molecular; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000494:pharmacology; D029607:Seminal Vesicle Secretory Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*; D013081:Sperm Motility / Q000187:drug effects*
D011994:Recombinant Proteins; D029607:Seminal Vesicle Secretory Proteins; C424482:seminal vesicle-specific antigen