Two alternatively spliced transcripts generated from OsMUS81, a rice homolog of yeast MUS81, are up-regulated by DNA-damaging treatments.
OsMUS81, a rice homolog of the yeast MUS81 endonuclease gene, produced two alternative transcripts, OsMUS81alpha and OsMUS81beta. OsMus81alpha contained a Helix-hairpin-Helix (HhH) motif at the N- and C-termini, and a conserved XPF-like motif in the center, while the OsMus81beta isoform lacked the second HhH motif by alternative splicing of a cryptic intron generating a truncated protein. The two transcripts were induced after DNA-damaging treatments such as high intensity light, UV-C and gamma-radiation. The yeast two-hybrid assay detected a strong interaction between OsMus81 and OsRad54 recombinational repair proteins. These findings suggest that OsMus81 functions in maintaining genome integrity through homologous recombination.
Mimida
Naozumi
N
National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, 305-8602 Japan.
Kitamoto
Hiroko
H
Osakabe
Keishi
K
Nakashima
Marina
M
Ito
Yuji
Y
Heyer
Wolf-Dietrich
WD
Toki
Seiichi
S
Ichikawa
Hiroaki
H
D017398:Alternative Splicing / Q000235:genetics* / Q000528:radiation effects; D000595:Amino Acid Sequence; D001483:Base Sequence; D004249:DNA Damage / Q000528:radiation effects; D018744:DNA, Plant; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D004720:Endonucleases / Q000737:chemistry / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D012275:Oryza / Q000235:genetics* / Q000528:radiation effects; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics*; D017385:Sequence Homology; D020798:Two-Hybrid System Techniques; D015854:Up-Regulation / Q000235:genetics* / Q000528:radiation effects
D018744:DNA, Plant; D004268:DNA-Binding Proteins; D010940:Plant Proteins; D029701:Saccharomyces cerevisiae Proteins; D004720:Endonucleases; C411624:MUS81 protein, S cerevisiae