title: Effects of contemporary troponin assay sensitivity on the utility of the early markers myoglobin and CKMB isoforms in evaluating patients with possible acute myocardial infarction.
abstract: BACKGROUND
  The 2003 American Heart Association (AHA) definition for myocardial infarction (MI) requires an "adequate set" (i.e. at least 6 h between measurements) of biomarkers and specifically troponin for the diagnosis of MI. The aim of the present study was to assess the performance of myoglobin, the CKMB isoforms, and cardiac troponin I (cTnI) in specimens earlier than the requisite 6 h after presentation, in a population originally characterized using World Health Organization (WHO) criteria.
  
  
  METHODS
  In 1996, 228 acute coronary syndrome patients with an "adequate sample set" had their specimens assayed for CKMB isoforms and myoglobin. In 2003, the same specimens were analyzed with the AccuTnI troponin I assay and myoglobin (Beckman Coulter Access immunoassay).
  
  
  RESULTS
  The clinical sensitivities for both myoglobin and the CKMB isoforms were >90% when the population was classified by WHO criteria. However the sensitivities were <70% when the ESC/ACC MI definition was used. Analyzing cTnI at earlier time points as long as there was at least 3 h between specimens or at least 1 specimen 6 h from pain onset did not misclassify subjects based on adverse outcomes in the year following their presentation.
  
  
  CONCLUSIONS
  Contemporary assays for cTnI with increased analytical sensitivity reduce the utility of myoglobin and CKMB isoforms to rule-out an AMI.
authors.affiliation: Department of Pathology and Molecular Medicine, McMaster University Medical Centre, 1200 Main St. W., HSC 2N52, Hamilton, ON, Canada L8N 3Z5. kavsakp@mcmaster.ca
authors.forename: Peter A
authors.initials: PA
authors.lastname: Kavsak
authors.forename: Andrew R
authors.initials: AR
authors.lastname: MacRae
authors.forename: Alice M
authors.initials: AM
authors.lastname: Newman
authors.forename: Viliam
authors.initials: V
authors.lastname: Lustig
authors.forename: Glenn E
authors.initials: GE
authors.lastname: Palomaki
authors.forename: Dennis T
authors.initials: DT
authors.lastname: Ko
authors.forename: Jack V
authors.initials: JV
authors.lastname: Tu
authors.forename: Allan S
authors.initials: AS
authors.lastname: Jaffe
mesh_terms: D000208:Acute Disease; D000368:Aged; D015415:Biomarkers / Q000097:blood*; D052279:Creatine Kinase, MB Form / Q000097:blood*; D004562:Electrocardiography; D005260:Female; D006801:Humans; D007118:Immunoassay; D007527:Isoenzymes / Q000097:blood; D008297:Male; D009203:Myocardial Infarction / Q000097:blood / Q000175:diagnosis*; D009211:Myoglobin / Q000097:blood*; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D019210:Troponin I / Q000097:blood*
chemical_list: D015415:Biomarkers; D007527:Isoenzymes; D009211:Myoglobin; D019210:Troponin I; D052279:Creatine Kinase, MB Form