DNA/Lipid complex incorporated with fibronectin to cell adhesion enhances transfection efficiency in prostate cancer cells and xenografts.
Previously we have described the development and applications of lipid-based nanoparticles for gene delivery vector. In an attempt to improve transfection efficiency using the cell adhesion of extracellular matrix (ECM) to DNA/lipid complex (nanoplex), the mRNA expression of integrin alpha2beta1 and CD44 in prostate cancer cells was detected as adhesion molecules for fibronectin (Fn), collagen I (Col) and laminin (Lam) using a commercially available cDNA array (GEArray) system. These ECM proteins could enhance DNA transfection activity in cells when coated on the nanoplex. Among the ECM proteins, Fn-coating nanoplexes significantly increased transfection activity 2-fold in prostate cancer PC-3 cells, and exhibited higher DNA transfection activities to PC-3 xenografts, compared with commercially available cationic polymer in vivo jetPEI. These results indicated that Fn-coating nanoplexes could facilitate efficient transfection of prostate tumor cells.
Hattori
Yoshiyuki
Y
Institute of Medicinal Chemistry, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan. yoshie@hoshi.ac.jp
Maitani
Yoshie
Y
D000818:Animals; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000235:genetics; D045744:Cell Line, Tumor; D024042:Collagen Type I / Q000737:chemistry / Q000235:genetics; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D005353:Fibronectins / Q000737:chemistry / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D018960:Hyaluronan Receptors / Q000737:chemistry / Q000235:genetics; D038982:Integrin alpha2beta1 / Q000737:chemistry / Q000235:genetics; D007797:Laminin / Q000737:chemistry / Q000235:genetics; D008055:Lipids / Q000737:chemistry*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053758:Nanoparticles; D010316:Particle Size; D011471:Prostatic Neoplasms / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014162:Transfection / Q000379:methods*; D023041:Xenograft Model Antitumor Assays / Q000379:methods
D015815:Cell Adhesion Molecules; D024042:Collagen Type I; D005353:Fibronectins; D018960:Hyaluronan Receptors; D038982:Integrin alpha2beta1; D007797:Laminin; D008055:Lipids; D012333:RNA, Messenger; D004247:DNA