title: Depolarization and cardiolipin depletion in aged rat brain mitochondria: relationship with oxidative stress and electron transport chain activity.
abstract: A noticeable loss of cardiolipin, a significant accumulation of fluorescent products of lipid peroxidation and an increased ability to produce reactive oxygen species in vitro are characteristics of aged rat brain mitochondria, as has been demonstrated in this study. In contrast mitochondrial electron transport chain activity is not significantly compromised except a marginal decline in complex IV activity in aged rat brain. On the other hand, a striking loss of mitochondrial membrane potential occurs in brain mitochondria during aging, which may be attributed to peroxidative membrane damage in this condition. Such mitochondrial dysfunctions as reported here may lead to uncoupling of oxidative phosphorylation, ATP depletion and activation of apoptotic cascade in aged rat brain.
authors.affiliation: Department of Biochemistry, Dr. B.C. Roy Post-graduate Institute of Basic Medical Sciences & IPGMER, 244B, Acharya J.C. Bose Road, Calcutta 700020, India.
authors.forename: Tanusree
authors.initials: T
authors.lastname: Sen
authors.forename: Nilkantha
authors.initials: N
authors.lastname: Sen
authors.forename: Sirsendu
authors.initials: S
authors.lastname: Jana
authors.forename: Firoj Hossain
authors.initials: FH
authors.lastname: Khan
authors.forename: Uttara
authors.initials: U
authors.lastname: Chatterjee
authors.forename: Sasanka
authors.initials: S
authors.lastname: Chakrabarti
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D002308:Cardiolipins / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000378:metabolism*; D005260:Female; D008297:Male; D008564:Membrane Potentials; D008928:Mitochondria / Q000378:metabolism*; D018384:Oxidative Stress; D051381:Rats
chemical_list: D002308:Cardiolipins; D003576:Electron Transport Complex IV