title: Study of galactomannose interaction with solids using AFM, IR and allied techniques.
abstract: Guar gum (GG) and locust bean gum (LBG) are two galactomannose polysaccharides with different mannose/galactose ratio which is widely used in many industrial sectors including food, textiles, paper, adhesive, paint, pharmaceuticals, cosmetics and mineral processing. They are natural nonionic polymers that are non-toxic and biodegradable. These properties make them ideal for industrial applications. However, a general lack of understanding of the interactions between the polysaccharides and solid surfaces has hindered wider application of these polymers. In this work, adsorption of locust bean gum and guar gum at the solid-liquid interface was investigated using adsorption tests, electrophoretic mobility measurements, FTIR, fluorescence spectroscopy, AFM and molecular modeling. Electrokinetic studies showed that the adsorption of GG and LBG on talc do not change its isoelectric point. In addition, GG and LBG adsorption on talc was found not to be affected by changes in solution conditions such as pH and ionic strength, which suggests a minor role of electrostatic force in adsorption. On the other hand, fluorescence spectroscopy studies conducted to investigate the role of hydrophobic bonding using pyrene probe showed no evidence of the formation of hydrophobic domains at talc-aqueous interface. Moreover, urea, a hydrogen bond breaker, markedly reduced the adsorption of LBG and GG on talc, supporting hydrogen bonding as an important role. In FTIR study, the changes in the infrared bands, associated with the CO stretch coupled to the CC stretch and OH deformation, were significant and therefore also supporting hydrogen bonding of GG and LBG to the solid surface. In addition, Langmuir modeling of adsorption isotherm further suggested that hydrogen bonding is the dominant force for polysaccharide adsorption since the adsorption free energy of these polymers is close to that for hydrogen bond formation. From molecular modeling, different helical structures are observed for LBG and GG because of their different galactose/mannose ratio and these polymers were found to adsorb flat on solid to let more of its OH groups in contact with the surface. All of the above results suggest that the main driving force for adsorption both of GG and LBG on talc is hydrogen bonding rather than hydrophobic force even though there is difference in G/M ratio between them.
authors.affiliation: NSF Industrial/University Cooperative Research Center for Advanced Studies in Novel Surfactants, Columbia University, New York, NY 10027, USA.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Ponisseril
authors.initials: P
authors.lastname: Somasundaran
mesh_terms: D000327:Adsorption; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004563:Electrochemistry; D005685:Galactans / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008351:Mannans / Q000737:chemistry*; D018625:Microscopy, Atomic Force / Q000379:methods*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009994:Osmolar Concentration; D010316:Particle Size; D053149:Plant Gums / Q000737:chemistry*; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013499:Surface Properties
chemical_list: D005685:Galactans; D008351:Mannans; D053149:Plant Gums; C007894:guar gum; C017471:locust bean gum