title: Optimization of culture of Leptospira from humans with leptospirosis.
abstract: A prospective study of 989 patients with acute febrile illness was performed in northeast Thailand to define the yield of Leptospira from four different types of blood sample. Based on a comparison of the yields from whole blood, surface plasma, deposit from spun plasma, and clotted blood samples from 80 patients with culture-proven leptospirosis, we suggest a sampling strategy in which culture is performed using whole blood and deposit from spun plasma.
authors.affiliation: Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Road, Bangkok 10400, Thailand. lek@tropmedres.ac
authors.forename: Vanaporn
authors.initials: V
authors.lastname: Wuthiekanun
authors.forename: Wirongrong
authors.initials: W
authors.lastname: Chierakul
authors.forename: Direk
authors.initials: D
authors.lastname: Limmathurotsakul
authors.forename: Lee D
authors.initials: LD
authors.lastname: Smythe
authors.forename: Meegan L
authors.initials: ML
authors.lastname: Symonds
authors.forename: Michael F
authors.initials: MF
authors.lastname: Dohnt
authors.forename: Andrew T
authors.initials: AT
authors.lastname: Slack
authors.forename: Roongreung
authors.initials: R
authors.lastname: Limpaiboon
authors.forename: Yupin
authors.initials: Y
authors.lastname: Suputtamongkol
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: White
authors.forename: Nicholas P J
authors.initials: NP
authors.lastname: Day
authors.forename: Sharon J
authors.initials: SJ
authors.lastname: Peacock
mesh_terms: D001431:Bacteriological Techniques; D001769:Blood / Q000382:microbiology*; D006801:Humans; D007919:Leptospira / Q000254:growth & development* / Q000302:isolation & purification*; D007922:Leptospirosis / Q000382:microbiology*; D012680:Sensitivity and Specificity; D013048:Specimen Handling; D013785:Thailand