Aluminum hydroxide associated to Schistosoma mansoni 22.6 kDa protein abrogates partial protection against experimental infection but not alter interleukin-10 production.
The need to develop a vaccine against schistosomiasis led several researches and our group to investigate proteins from Schistosoma mansoni as vaccine candidates. Sm22.6 is a protein from S. mansoni that shows high identity with Sj22.6 and Sh22.6 (79 and 91%, respectively). These proteins are associated with high levels of IgE and protection to reinfection. Previously, we have shown that Sm22.6 induced a partial protection of 34.5% when used together with Freund's adjuvant and produced a Th0 type of immune response with interferon-g and interleukin-4. In this work, mice were immunized with Sm22.6 alone or with aluminum hydroxide adjuvant and high levels of IgG, IgG1, and IgG2a were measured. Unfortunately, no protection was detected. Since IL-10 is a modulating cytokine in schistosomiasis, we also observed a high level of this molecule in splenocytes of vaccinated mice. In conclusion, we did not observe the adjuvant effect of aluminum hydroxide associated with rSm22.6 in protective immunity.
Pacífico
Lucila G G
LG
Departamento de Bioquímica e Imunologia, Instituto de Investigação em Imunologia, Instituto Millennium, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
Fonseca
Cristina T
CT
Barsante
Michele M
MM
Cardoso
Luciana S
LS
Araújo
Maria Ilma
MI
Oliveira
Sérgio C
SC
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000536:Aluminum Hydroxide / Q000008:administration & dosage*; D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D004195:Disease Models, Animal; D005260:Female; D015801:Helminth Proteins / Q000008:administration & dosage*; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology; D016753:Interleukin-10 / Q000096:biosynthesis*; D051379:Mice; D008810:Mice, Inbred C57BL; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000276:immunology* / Q000517:prevention & control
D000276:Adjuvants, Immunologic; D000909:Antibodies, Helminth; D015801:Helminth Proteins; D007074:Immunoglobulin G; D016753:Interleukin-10; D000536:Aluminum Hydroxide