[Mean level of expression of c-myb gene in leukaemia of children].
OBJECTIVE
Measurement of c-myb expression in leukaemia cells in children and in normal cells of healthy controls.


METHODS
37 patients, 23 boys and 14 girls with acute leukaemia, aged 1-17 years, were included in the study (32 with acute lymphoblastic leukaemia and 5 with acute myeloblasts leukaemia) Control group consisted of 17 healthy children, 8 boys and 9 girls, 4-18 years old. After the isolation of mononuclear cells from bone marrow I peripheral blood mRNA was isolated, then with the use of reverse transcriptase cDNA was synthesized. The level of expression of c-myb was analyzed with polymerase chain reaction method. The final result was analyzed as the ratio between fluorescence of c-myb gene and the control gene.


RESULTS
Mean level of expression of c-myb gene in leukaemia cells was statistically significantly higher than in the control group (0.71+/-0.53 vs. 0.51+/-0.22; p=0.05), as well as c-myb level between leukaemia cells in relapse cases and controls (0.83+/-0.23 vs. 0.51+/-0.22; p=0.01). There was no difference between c-myb expression in different diagnosis. The comparison of c-myb expression in good and poor response group was not statistically significant (p=0.33).


CONCLUSIONS
Possible influence of increased expression of c-myb gene in the promotion of leukaemia was found. The role of c-myb expression as a prognostic factor in acute leukaemias of children was not confirmed.
Szmyd
Krzysztof
K
Klinika Transplantacji Szpiku, Onkologii i Hematologii Dzieciecej, ul. Bujwida 44, 50-345 Wrocław, Poland. krzysztofszmyd@yahoo.com
Chaber
Radoslaw
R
Fiszer-Maliszewska
Lucja
L
Reich
Adam
A
Grotthus
Elzbieta
E
Weclawek-Tompol
Jadwiga
J
Chybicka
Alicja
A
D000293:Adolescent; D001853:Bone Marrow / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D011044:Poland; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000378:metabolism; D020598:Proto-Oncogene Proteins c-myb / Q000378:metabolism*; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity
D020598:Proto-Oncogene Proteins c-myb