title: Odontogenic potential of bone marrow mesenchymal stem cells.
abstract: OBJECTIVE
  This study aimed to investigate the odontogenic potential of bone marrow mesenchymal stem cells (BM-MSCs) for seeding in tooth regeneration.
  
  
  METHODS
  In this study, BM-MSCs were co-cultured with oral epithelial cells derived from rat embryos. Expression of the odontogenic genes Pax9, DMP1, and DSPP was detected by the reverse-transcription polymerase chain reaction (RT-PCR) technique. To further characterize the odontogenic potential of BM-MSCs, the gold standard in vivo transplantation system was used.
  
  
  RESULTS
  The results revealed that Pax9, DMP1, and DSPP expression was detected by RT-PCR only after co-culture of BM-MSCs and oral epithelial cells derived from embryos age E11.5. Histological analyses of the BM-MSCs/epithelial cell mass demonstrated the presence of tooth-like structures.
  
  
  CONCLUSIONS
  The series of experiments both in vitro and in vivo demonstrated that BM-MSCs can differentiate into functional odontoblast-like cells. This implies that BM-MSCs may become a novel source of cells for seeding in tooth regeneration research.
authors.affiliation: Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Li
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Yun-Feng
authors.initials: YF
authors.lastname: Lin
authors.forename: Sheng-Wei
authors.initials: SW
authors.lastname: Li
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Tian
mesh_terms: D053687:Adult Stem Cells / Q000502:physiology / Q000637:transplantation; D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D018920:Coculture Techniques; D004622:Embryo, Mammalian / Q000166:cytology; D004847:Epithelial Cells / Q000502:physiology* / Q000637:transplantation; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D009055:Mouth / Q000166:cytology; D009804:Odontoblasts / Q000166:cytology / Q000502:physiology; D009805:Odontogenesis / Q000235:genetics / Q000502:physiology*; D051764:PAX9 Transcription Factor / Q000096:biosynthesis; D010750:Phosphoproteins / Q000096:biosynthesis; D011498:Protein Precursors / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein
chemical_list: D016326:Extracellular Matrix Proteins; D051764:PAX9 Transcription Factor; D010750:Phosphoproteins; D011498:Protein Precursors; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein; C081635:Dmp1 protein, rat