title: A water-soluble, octacationic zinc phthalocyanine as molecular probe for nucleic acid secondary structure.
abstract: The interaction between CT-DNA and the zinc phthalocyanine ZnPc (1) was studied by UV/VIS and fluorescence titration, as well as by thermal denaturation. ZnPc was found to strongly bind to CT-DNA (K(app)=7.35 x 10(5) M(-1)) in a non-intercalative mode. The photosensitized cleavage of pBR322 DNA was found to efficiently proceed via singlet-oxygen ((1)O(2)) production. Further, ZnPc (1) caused site-specific scission of guanine (G) bases around the bulge of the hairpin oligonucleotides OD1-OD3, as clearly shown by gel-electrophoresis experiments.
authors.affiliation: College of Chemistry and Molecular Sciences, Wuhan University, Hubei, Wuhan, PR China.
authors.forename: An-Ming
authors.initials: AM
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Huang
authors.forename: Xiao-Cheng
authors.initials: XC
authors.lastname: Weng
authors.forename: Jin-Xi
authors.initials: JX
authors.lastname: Li
authors.forename: Li-Ge
authors.initials: LG
authors.lastname: Ren
authors.forename: Zhi-Bin
authors.initials: ZB
authors.lastname: Song
authors.forename: Xiao-Qin
authors.initials: XQ
authors.lastname: Xiong
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Cao
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D004247:DNA / Q000378:metabolism*; D007211:Indoles / Q000378:metabolism*; D054833:Isoindoles; D015335:Molecular Probes; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009942:Organometallic Compounds / Q000378:metabolism*; D010782:Photolysis; D013056:Spectrophotometry, Ultraviolet; D017967:Zinc Compounds
chemical_list: D007211:Indoles; D054833:Isoindoles; D015335:Molecular Probes; D009942:Organometallic Compounds; D017967:Zinc Compounds; C052159:Zn(II)-phthalocyanine; D004247:DNA