Nonradioactive northern and Southern analyses from plant samples.
Several specific problems are encountered when nonradioactive detection methods are used in conjunction with plant nucleic acids. In this chapter, we describe protocols for the isolation of DNA and RNA from plant leaves and the preparation of probe molecules by either PCR or in vitro transcription with different haptens. Furthermore, standard conditions and possible modifications for hybridization and detection of probes are given.
Peterhaensel
Christoph
C
RWTH Aachen University, Biology I, Germany.
Weier
Dagmar
D
Lahaye
Thomas
T
D001710:Biotin; D015152:Blotting, Northern / Q000379:methods*; D015139:Blotting, Southern / Q000379:methods*; D002021:Buffers; D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D018744:DNA, Plant / Q000235:genetics* / Q000302:isolation & purification*; D004076:Digoxigenin; D004587:Electrophoresis, Agar Gel; D019793:Fluorescein; D006146:Guanidines; D008967:Molecular Biology / Q000379:methods; D015336:Molecular Probe Techniques; D010636:Phenols; D018515:Plant Leaves / Q000737:chemistry; D016133:Polymerase Chain Reaction; D018749:RNA, Plant / Q000235:genetics* / Q000302:isolation & purification*; D014158:Transcription, Genetic
D002021:Buffers; D002593:Cetrimonium Compounds; D018744:DNA, Plant; D006146:Guanidines; D010636:Phenols; D018749:RNA, Plant; C411644:trizol; D001710:Biotin; D004076:Digoxigenin; D019793:Fluorescein; D000077286:Cetrimonium