Effect of local antimicrobial agents on excisional palatal wound healing: a clinical and histomorphometric study in rats.
OBJECTIVE
To assess the effect of topically applied antimicrobial agents on palatal excisional wound in rats.


METHODS
Excisional wounds, 5 mm in diameter, were made in the centre of the palate of 125 Wistar male rats. In four experimental groups, chlorhexidine digluconate (CHX) 0.12% solution, 1% CHX gel, phenolic compounds solution (Listerine), amine/stannous fluoride solution (Meridol) and saline solution as a control group were applied daily for 1 min. The wound area was measured photographically and the epithelialization rate was determined histologically at 3, 7, 14 and 21 days post-surgery.


RESULTS
The mean wound area and mean distance between the epithelial margins decreased significantly with time (p<0.001) in experimental and control groups, with the greatest wound area reduction and rate of epithelialization on day 14. A significantly superior rate of wound epithelialization (p=0.03) was presented following use of 1% CHX gel and Listerine and a comparatively inferior one when the Meridol solution was applied.


CONCLUSIONS
Each tested antimicrobial agent when applied on an excisional wound with epithelial and connective tissue deficiency did not have a negative effect on the rate of wound closure. The best results were achieved with 1%CHX gel and Listerine.
Kozlovsky
Avital
A
Department of Periodontology, The Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv, Israel. kavital@post.tau.ic.il
Artzi
Zvi
Z
Hirshberg
Avraham
A
Israeli-Tobias
Chen
C
Reich
Liat
L
D000588:Amines / Q000627:therapeutic use; D000818:Animals; D000891:Anti-Infective Agents, Local / Q000627:therapeutic use*; D002710:Chlorhexidine / Q000031:analogs & derivatives / Q000627:therapeutic use; D003238:Connective Tissue / Q000187:drug effects / Q000502:physiology; D003239:Connective Tissue Cells / Q000187:drug effects; D004338:Drug Combinations; D004847:Epithelial Cells / Q000187:drug effects* / Q000502:physiology; D008297:Male; D009061:Mouth Mucosa / Q000166:cytology / Q000187:drug effects; D010159:Palate / Q000166:cytology / Q000187:drug effects / Q000502:physiology* / Q000601:surgery; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration / Q000187:drug effects*; D012459:Salicylates / Q000627:therapeutic use; D013729:Terpenes / Q000627:therapeutic use; D014002:Tin Fluorides / Q000627:therapeutic use; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects*
D000588:Amines; D000891:Anti-Infective Agents, Local; D004338:Drug Combinations; D012459:Salicylates; D013729:Terpenes; D014002:Tin Fluorides; C059813:Meridol; C057473:Listerine; C010882:chlorhexidine gluconate; D002710:Chlorhexidine