title: Identification of acid-sensing ion channels in adenoid cystic carcinomas.
abstract: Tissue acidosis is an important feature of tumor. The response of adenoid cystic carcinoma (ACC) cells to acidic solution was studied using whole-cell patch-clamp recording in the current study. An inward, amiloride-sensitive Na(+) current was identified in cultured ACC-2 cells while not in normal human salivary gland epithelial cells. Electrophysiological and pharmacological properties of the currents suggest that heteromeric acid-sensing ion channels (ASICs) containing 2a and 3 may be responsible for the proton-induced currents in the majority of ACC-2 cells. Consistent with it, analyses of RT-PCR and Western blotting demonstrated the presences of ASIC2a and 3 in ACC-2 cells. Furthermore, we observed the enhanced expression of ASIC2a and 3 in the sample of ACC tissues. These results indicate that the functional expression of ASICs is characteristic feature of ACC cells.
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Stomatology, Affiliated Ninth People's Hospital, Shanghai Jiaotong University, Shanghai Research Institute of Stomatology, Shanghai 200011, China.
authors.forename: Jin-Hai
authors.initials: JH
authors.lastname: Ye
authors.forename: Jun
authors.initials: J
authors.lastname: Gao
authors.forename: Yu-Nong
authors.initials: YN
authors.lastname: Wu
authors.forename: Yong-Jie
authors.initials: YJ
authors.lastname: Hu
authors.forename: Chen-Ping
authors.initials: CP
authors.lastname: Zhang
authors.forename: Tian-Le
authors.initials: TL
authors.lastname: Xu
mesh_terms: D062565:Acid Sensing Ion Channels; D003528:Carcinoma, Adenoid Cystic / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D004594:Electrophysiology; D005786:Gene Expression Regulation; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D011522:Protons; D012469:Salivary Glands / Q000378:metabolism; D015222:Sodium Channels / Q000235:genetics / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism
chemical_list: D062565:Acid Sensing Ion Channels; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D011522:Protons; D015222:Sodium Channels; D015032:Zinc