PSF is an IbeA-binding protein contributing to meningitic Escherichia coli K1 invasion of human brain microvascular endothelial cells.
During the development of Escherichia coli K1 meningitis, interaction between E. coli invasion protein IbeA and surface protein(s) on human brain microvascular endothelial cells (HBMEC) is required for invasion and IbeA-mediated signaling. Here, an IbeA-binding protein was identified as polypyrimidine tract-binding protein (PTB)-associated splicing factor (PSF). The specific binding was confirmed by ligand overlay assay. The cell surface-expressed PSF was verified by the confocal microscopy. Recombinant PSF blocked E. coli K1 invasion of HBMEC effectively. Overexpression of PSF in the lentivirus-transducted HBMEC significantly enhanced E. coli K1 invasion. These results suggest that IbeA interacts with PSF for the E. coli K1 invasion of HBMEC.
Zou
Yanming
Y
Saban Research Institute, Childrens Hospital Los Angeles, Los Angeles, CA 90027, USA.
He
Lina
L
Wu
Chun-Hua
CH
Cao
Hong
H
Xie
Zhi-Hua
ZH
Ouyang
Yannan
Y
Wang
Yang
Y
Jong
Ambrose
A
Huang
Sheng-He
SH
D000595:Amino Acid Sequence; D001812:Blood-Brain Barrier; D001921:Brain / Q000098:blood supply / Q000382:microbiology*; D002460:Cell Line; D042783:Endothelial Cells / Q000382:microbiology*; D004926:Escherichia coli / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D000072670:PTB-Associated Splicing Factor; D011485:Protein Binding; D016601:RNA-Binding Proteins / Q000378:metabolism*
C096350:CusC protein, E coli; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D000072670:PTB-Associated Splicing Factor; D016601:RNA-Binding Proteins