title: The structure of Plasmodium vivax phosphatidylethanolamine-binding protein suggests a functional motif containing a left-handed helix.
abstract: The structure of a putative Raf kinase inhibitor protein (RKIP) homolog from the eukaryotic parasite Plasmodium vivax has been studied to a resolution of 1.3 A using multiple-wavelength anomalous diffraction at the Se K edge. This protozoan protein is topologically similar to previously studied members of the phosphatidylethanolamine-binding protein (PEBP) sequence family, but exhibits a distinctive left-handed alpha-helical region at one side of the canonical phospholipid-binding site. Re-examination of previously determined PEBP structures suggests that the P. vivax protein and yeast carboxypeptidase Y inhibitor may represent a structurally distinct subfamily of the diverse PEBP-sequence family.
authors.affiliation: Structural Genomics of Pathogenic Protozoa Consortium, USA.
authors.forename: Tracy
authors.initials: T
authors.lastname: Arakaki
authors.forename: Helen
authors.initials: H
authors.lastname: Neely
authors.forename: Erica
authors.initials: E
authors.lastname: Boni
authors.forename: Natasha
authors.initials: N
authors.lastname: Mueller
authors.forename: Frederick S
authors.initials: FS
authors.lastname: Buckner
authors.forename: Wesley C
authors.initials: WC
authors.lastname: Van Voorhis
authors.forename: Angela
authors.initials: A
authors.lastname: Lauricella
authors.forename: George
authors.initials: G
authors.lastname: DeTitta
authors.forename: Joseph
authors.initials: J
authors.lastname: Luft
authors.forename: Wim G J
authors.initials: WG
authors.lastname: Hol
authors.forename: Ethan A
authors.initials: EA
authors.lastname: Merritt
mesh_terms: D020816:Amino Acid Motifs / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites / Q000502:physiology; D018360:Crystallography, X-Ray; D008969:Molecular Sequence Data; D049488:Phosphatidylethanolamine Binding Protein / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology*; D010966:Plasmodium vivax / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D047428:Protein Kinase Inhibitors / Q000737:chemistry / Q000378:metabolism; D017433:Protein Structure, Secondary / Q000502:physiology; D015800:Protozoan Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology
chemical_list: D049488:Phosphatidylethanolamine Binding Protein; D047428:Protein Kinase Inhibitors; D015800:Protozoan Proteins