Near full-length genome characterization of three additional HIV type 1 CRF13_cpx strains from Cameroon.
Recombinant forms of HIV-1 contribute significantly to the ongoing epidemic. In the present study, we characterized the near full-length genomes of three candidate HIV-1 CRF13_cpx strains originating in Cameroon, 04CM-173-9, 04CM-632-28, and 02CM-A1394. Bootscanning, recombination breakpoint analysis, and phylogenetic trees confirmed similar genomic structures with identical breakpoint positions compared to the three available CRF13_cpx sequences. The candidate and reference sequences formed a distinct cluster well separated from other group M subtypes and had a mosaic structure derived from subtypes A1, G, J, and CRF01_AE. The similarity in genomic composition and position of recombination breakpoints suggest that these isolates share a common ancestor. The epidemiological significance of CRF13_cpx strains in Cameroon is unknown; however, the availability of three additional genomic sequences will improve our understanding of the overall genetic diversity within this recombinant form of HIV-1.
Luk
Ka-Cheung
KC
Abbott Diagnostics, AIDS Research and Retrovirus Discovery, Abbott Laboratories, 100 Abbott Park Road, Abbott Park, IL 60064, USA.
Holzmayer
Vera
V
Yamaguchi
Julie
J
Swanson
Priscilla
P
Brennan
Catherine A
CA
Ngansop
Charlotte
C
Mbanya
Dora
D
Gayum
Hermine
H
Djuidje
Marcelline Ngounoue
MN
Ndembi
Nicaise
N
Kamdem
Donatien
D
Kaptué
Lazare
L
Gürtler
Lutz
L
Devare
Sushil G
SG
Hackett
John
J
D000328:Adult; D002163:Cameroon; D005260:Female; D015658:HIV Infections / Q000235:genetics*; D015497:HIV-1 / Q000145:classification / Q000235:genetics*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D016865:Reassortant Viruses / Q000145:classification / Q000235:genetics*; D017423:Sequence Analysis, RNA