Identification of cyclin D3 as a new interaction partner of lamin A/C.
Lamin A/C is a major component of the nuclear lamina. An intact nuclear lamina has been proposed to be necessary for muscle differentiation. Cyclin D3 is known to be upregulated in differentiated muscle cells and to form insoluble complexes with cell-cycle regulatory factors in these cells. We have examined the possibility of direct binding interactions between lamin A/C and cyclin D3 by in vitro binding assays and co-immunoprecipitation studies with muscle cells. Our results indicate that cyclin D3 binds specifically to amino acid residues 383-474 of lamin A/C and associates with lamin A/C in muscle cells. The identification of cyclin D3 as a novel binding partner of lamin A/C has important implications for a role for lamin A/C in muscle differentiation.
Mariappan
Indumathi
I
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
Gurung
Ritika
R
Thanumalayan
Subramonian
S
Parnaik
Veena K
VK
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D056743:Cyclin D3; D016213:Cyclins / Q000235:genetics / Q000378:metabolism*; D034904:Lamin Type A / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D011485:Protein Binding
C534697:Ccnd3 protein, mouse; D056743:Cyclin D3; D016213:Cyclins; D034904:Lamin Type A; C440308:lamin C