title: Cerebrospinal fluid viral loads reach less than 2 copies/ml in HIV-1-infected patients with effective antiretroviral therapy.
abstract: BACKGROUND
  A low-grade persisting viraemia despite long-term treatment with highly active antiretroviral therapy (HAART) has previously been demonstrated in HIV-1-infected patients. Whether ongoing viral replication also could be detected in cerebrospinal fluid (CSF) in those circumstances has not been studied before.
  
  
  METHODS
  Paired CSF and blood samples from 13 neurologically asymptomatic HIV-1-infected patients on stable HAART were analysed regarding HIV-1 RNA, by using a PCR assay with a detection limit of 2 copies/ml.
  
  
  RESULTS
  All 13 patients had HIV-1 RNA < 2 copies/ml in CSF, compared with 8/13 in plasma.
  
  
  CONCLUSIONS
  We could not demonstrate any persistent viral replication in the CSF of neurologically asymptomatic HIV-1-infected patients on effective HAART, rendering it unlikely that CSF acts as a viral reservoir in this category of patients.
authors.affiliation: Department of Infectious Diseases, The Sahlgrenska Academy at Göteborg University, Sweden. aylin.yilmaz@infect.gu.se
authors.forename: Aylin
authors.initials: A
authors.lastname: Yilmaz
authors.forename: Bo
authors.initials: B
authors.lastname: Svennerholm
authors.forename: Lars
authors.initials: L
authors.lastname: Hagberg
authors.forename: Magnus
authors.initials: M
authors.lastname: Gisslén
mesh_terms: D000368:Aged; D019380:Anti-HIV Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D023241:Antiretroviral Therapy, Highly Active; D005260:Female; D015658:HIV Infections / Q000188:drug therapy* / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000134:cerebrospinal fluid*; D016896:Treatment Outcome
chemical_list: D019380:Anti-HIV Agents; D012367:RNA, Viral