title: Background selection at the chitin synthase II (chs2) locus in Paracoccidioides brasiliensis species complex.
abstract: In fungi, chitin synthases have been classified into five classes according to differences in regions of high sequence conservation. The current investigation was initiated to examine the causes for the polymorphism patterns found in a class II chitin synthase gene (chs2) of Paracoccidioides brasiliensis, in an attempt to determine the evolutionary forces affecting the chitin synthesis metabolic pathway. Neutrality tests were applied to the chs2 sequences exhibited by P. brasiliensis species complex. According to these tests and based on non-synonymous differences, P. brasiliensis data rejected the null hypothesis for a pure drift mutational process owing to a large excess of unique polymorphisms. In contrast, the synonymous and intron site differences did not reject the null hypothesis. This pattern appears consistent with weak selection against most amino acid changes, in which the effect of background selection was not detectable at synonymous nor at intron sites.
authors.affiliation: Corporación para Investigaciones Biológicas (CIB), Medellín, Colombia. dmatute@uchicago.edu
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Matute
authors.forename: Isaura P
authors.initials: IP
authors.lastname: Torres
authors.forename: Catalina
authors.initials: C
authors.lastname: Salgado-Salazar
authors.forename: Angela
authors.initials: A
authors.lastname: Restrepo
authors.forename: Juan G
authors.initials: JG
authors.lastname: McEwen
mesh_terms: D002687:Chitin Synthase / Q000235:genetics*; D005787:Gene Frequency; D006239:Haplotypes; D008957:Models, Genetic; D010228:Paracoccidioides / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D010802:Phylogeny; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D002687:Chitin Synthase; C400821:chitin synthase 2