title: [Differential analysis of two-dimensional gel electrophoresis profiles of spermatozoal protein in human normal semen and idiopathic asthenospermia].
abstract: OBJECTIVE
  To evaluate the application of two-dimensional electrophoresis in the research of differentially expressed proteins in human spermatozoa.
  
  
  METHODS
  Two-dimensional gel electrophoresis was performed on 4 sperm samples from normal healthy men and another 4 from asthenospermia patients. After silver staining, the differential expression proteins were analyzed by PDQuest 2D analysis software.
  
  
  RESULTS
  Seven differential protein spots were identified, 2 expressed highly in the asthenospermia sperm but lowly in the normal spermatozoa, while the other 5 expressed just the opposite way.
  
  
  CONCLUSIONS
  The protein profiles of differential expression between the normal spermatozoa and idiopathic asthenospermia were established and some differential proteins were found. The data obtained in this study may help prepare the ground for further studies on the isolation and identification of differentially expressed proteins in human asthenospermia sperm.
authors.affiliation: Department of Urology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China. shenforest@163.com
authors.forename: Shu-lin
authors.initials: SL
authors.lastname: Shen
authors.forename: Yong
authors.initials: Y
authors.lastname: Luo
authors.forename: Liang
authors.initials: L
authors.lastname: Ning
authors.forename: Da-lin
authors.initials: DL
authors.lastname: He
mesh_terms: D016022:Case-Control Studies; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008297:Male; D009845:Oligospermia / Q000378:metabolism*; D029589:Seminal Plasma Proteins / Q000032:analysis / Q000096:biosynthesis*; D013094:Spermatozoa / Q000737:chemistry
chemical_list: D029589:Seminal Plasma Proteins