title: Synthesis of two carboxylic haptens for raising antibodies to 25-hydroxyvitamin D3 and 1alpha,25-dihydroxyvitamin D3.
abstract: Hapten derivatives of 25-hydroxyvitamin D(3) and 1alpha,25-dihydroxyvitamin D(3) were synthesized using the Wittig-Horner approach. Both haptens bearing a carboxylic group at the side chain that can be linked to a protein for raising antibodies of potential utility for the determination of 25-hydroxyvitamin D(3), 1alpha,25-dihydroxyvitamin D(3) and 1alpha-hydroxylated vitamin D(3) analogues.
authors.affiliation: Departamento de Química Orgánica y Unidad Asociada al C.S.I.C., Universidad de Santiago de Compostela, E-15706 Santiago de Compostela, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Gregorio
authors.forename: Silvina
authors.initials: S
authors.lastname: Eduardo
authors.forename: Luis C
authors.initials: LC
authors.lastname: Rodrigues
authors.forename: María A
authors.initials: MA
authors.lastname: Regueira
authors.forename: Ramón
authors.initials: R
authors.lastname: Fraga
authors.forename: Ricardo
authors.initials: R
authors.lastname: Riveiros
authors.forename: Miguel
authors.initials: M
authors.lastname: Maestro
authors.forename: Antonio
authors.initials: A
authors.lastname: Mouriño
mesh_terms: D002112:Calcifediol / Q000138:chemical synthesis* / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D006241:Haptens / Q000737:chemistry* / Q000276:immunology; D015394:Molecular Structure; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology / Q000378:metabolism
chemical_list: D006241:Haptens; C118756:dihydroxy-vitamin D3; D014807:Vitamin D; D002112:Calcifediol