title: [The experimental study on bone marrow stem cell transplantition combinated with bushen fang therapy for DMD].
abstract: OBJECTIVE
  To investigate the effect on bone marrow stem cell transplantition (BMST) combinated with Bushen fang therapy in mdx mice.
  
  
  METHODS
  Cultured the bone marrow cells of C56BL/6 in vitro and tranplante these cells to mdx mice after irradiation. Bushen fang was used to cure the mdx mice after BMST. The survivel rate, symptom of Graft Versus Host Disease (GVHD) and motor function of model mice following irradiation and transplantation were observed 4 or 8 months after BMST, prepared the freeze-splice of muscles of each group, and made HE staining. The dystrophin (DYS) was detected by SABC immunofluorescene.
  
  
  RESULTS
  Bushen fang could decrease the rate of centrally nucleated fibers (CNF), enhance expressional rate of DYS in different level and relieve the symptom of GVHD in transplanted mice.
  
  
  CONCLUSIONS
  Bushen fang can improve the therapeutic efficacy of BMST.
authors.affiliation: Department of Neurology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China.
authors.forename: Xi-lin
authors.initials: XL
authors.lastname: Lu
authors.forename: Qun
authors.initials: Q
authors.lastname: Li
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Yao
authors.forename: Wei-xi
authors.initials: WX
authors.lastname: Zhang
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000818:Animals; D003131:Combined Modality Therapy; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D016189:Dystrophin / Q000096:biosynthesis; D005260:Female; D006086:Graft vs Host Disease / Q000473:pathology; D018380:Hematopoietic Stem Cell Transplantation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D018482:Muscle, Skeletal / Q000378:metabolism / Q000473:pathology; D020388:Muscular Dystrophy, Duchenne / Q000188:drug therapy / Q000401:mortality / Q000628:therapy*; D008517:Phytotherapy; D010946:Plants, Medicinal / Q000737:chemistry; D015996:Survival Rate
chemical_list: D004365:Drugs, Chinese Herbal; D016189:Dystrophin