Coordinated and selective recruitment of eIF4E and eIF4G factors for potyvirus infection in Arabidopsis thaliana.
The translation initiation factors eIF4E and eIF(iso)4E play a key role during virus infection in plants. During mRNA translation, eIF4E provides the cap-binding function and is associated with the protein eIF4G to form the eIF4F complex. Susceptibility analyses of Arabidopsis mutants knocked-out for At-eIF4G genes showed that eIF4G factors are indispensable for potyvirus infection. The colonization pattern by a viral recombinant carrying GFP indicated that eIF4G is involved at a very early infection step. Like eIF4E, eIF4G isoforms are selectively recruited for infection. Moreover, the eIF4G selective involvement parallels eIF4E recruitment. This is the first report of a coordinated and selective recruitment of eIF4E and eIF4G factors, suggesting the whole eIF4F recruitment.
Nicaise
Valérie
V
Interactions Plante-Virus (IPV), UMR 1090, INRA Bordeaux 2, BP 81, F-33883 Villenave d'Ornon, France.
Gallois
Jean-Luc
JL
Chafiai
Faccila
F
Allen
Leah M
LM
Schurdi-Levraud
Valérie
V
Browning
Karen S
KS
Candresse
Thierry
T
Caranta
Carole
C
Le Gall
Olivier
O
German-Retana
Sylvie
S
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D039561:Eukaryotic Initiation Factor-4E / Q000235:genetics / Q000378:metabolism*; D039603:Eukaryotic Initiation Factor-4G / Q000235:genetics / Q000378:metabolism*; D017343:Genes, Plant; D005816:Genetic Complementation Test; D009154:Mutation; D010935:Plant Diseases / Q000235:genetics / Q000821:virology; D017800:Potyvirus / Q000235:genetics / Q000472:pathogenicity*; D014176:Protein Biosynthesis
D029681:Arabidopsis Proteins; D004279:DNA, Viral; D039561:Eukaryotic Initiation Factor-4E; D039603:Eukaryotic Initiation Factor-4G