hZG16, a novel human secreted protein expressed in liver, was down-regulated in hepatocellular carcinoma.
Here we reported a novel human secreted protein named as hZG16, with a Jacalin domain. Evolution analysis through comparing with the orthologs of other organisms suggested that ZG16 is a conserved gene under the purifying selection (d(N)/d(s)<1) in the evolution. Interestingly, Northern and dot blot analyses showed that hZG16 were highly expressed in adult liver, not in fetal liver, and moderately in gut, including jejunum, ileum, and colon, in which the tissue expression pattern of hZG16 was significantly dissimilar to that of mouse and rat orthologs that were uniquely expressed in spleen and pancreas, respectively. Unexpectedly, hZG16 was markedly down-regulated in hepatocellular carcinoma (HCC) as indicated by RT-PCR, Northern blot analysis and immunohistochemistry staining. However, the tunicamicin treatment and pulse-chase experiments showed that hZG16 protein had a similar molecular function with rZG16 that take part in glycoproteins' secretion in a bus mode.
Zhou
Yu-Bo
YB
Chinese National Human Genome Center at Shanghai, 351 Guo Shou-Jing Road, Shanghai 201203, China.
Cao
Jia-Bing
JB
Yang
Hong-Meng
HM
Zhu
Hong
H
Xu
Zhi-Gang
ZG
Wang
Ke-Sheng
KS
Zhang
Xin
X
Wang
Zhi-Qin
ZQ
Han
Ze-Guang
ZG
D000595:Amino Acid Sequence; D006528:Carcinoma, Hepatocellular / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D037102:Lectins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000737:chemistry / Q000378:metabolism*; D008113:Liver Neoplasms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D021381:Protein Transport; D014018:Tissue Distribution; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D006023:Glycoproteins; D037102:Lectins; C519401:ZG16 protein, human