The effect of vitamin e on splenocytes apoptosis of gamma- irradiated BALB/c mice.
Apoptosis, a physiologic mechanism to eliminate unwanted cells, is also induced by ionizing irradiation, through production of free radicals. It has been demonstrated that antioxidants such as vitamin E are able to protect cells from damage caused by free radicals. Taken together we found it reasonable to make an attempt to evaluate the protective effect of vitamin E against apoptosis. The irradiated mice received 1 Gy/day gamma radiation for one day (low dose) or for three successive days (high dose, 3Gy). The splenocytes were then isolated at 6, 14 and 24 h after exposure. DNA gel electrophoresis and DNA fragmentation assay were done in addition to the evaluation of splenocytes cytology.Our results showed that Vitamin E can reduce apoptosis against low dose irradiation. However it is not able to completely block programmed cell death in high dose irradiation.
Maroufi
Bahman
B
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran. ardestani@ibb.ut.ac.ir.
Kaboudanian Ardestani
Sussan
S
Kariminia
Amina
A
Naderimanesh
Hussain
H