title: Analysis of FMO genes and off flavour in pork.
abstract: Off flavours in pork sometimes produce tastes such as sourness, fishy, metallic or other non-typical flavours and are often caused by low pH. Loss of function mutations in flavin containing mono-oxygenase 3 (FMO3) are known to be associated with a fishy off flavour in both chicken eggs and cow's milk and a similar autosomal recessive disorder is present in humans resulting in a fishy odour. FMO3 is a member of a gene family that is clustered on human chromosome 1. Comparative mapping suggested that FMO3 and the remaining FMO genes (ex. FMO1 and FMO5) might map to the orthologous region on pig chromosome 9 (SSC9) where a quantitative trait locus (QTL) for off flavour was previously identified. Primers were designed to amplify FMO1, FMO3 and FMO5 gene fragments and several SNPs were discovered and genotyping tests developed. The genotypes from the Iowa State University Berkshire x Yorkshire resource population were used to linkage map FMO1 and FMO3 to SSC9 and FMO5 to pig chromosome 4 (SSC4). QTL and associations analyses were performed using the map containing FMO1 and FMO3. Results demonstrated that FMO3 and FMO1 mapped less than 1 cM away from the peak for the off flavour QTL previously detected on SSC9 and provide indications of an association between the FMO3 polymorphism and off flavour in pork.
authors.affiliation: Department of Animal Science and the Center for Integrated Animal Genomics, Iowa State University, Ames, IA 50011-3150, USA.
authors.forename: K L
authors.initials: KL
authors.lastname: Glenn
authors.forename: A M
authors.initials: AM
authors.lastname: Ramos
authors.forename: M F
authors.initials: MF
authors.lastname: Rothschild
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D033481:Chromosomes, Mammalian / Q000235:genetics*; D017931:DNA Primers; D005838:Genotype; D008460:Meat / Q000032:analysis*; D008969:Molecular Sequence Data; D010105:Oxygenases / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D012044:Regression Analysis; D017422:Sequence Analysis, DNA; D034421:Sus scrofa / Q000235:genetics*
chemical_list: D017931:DNA Primers; D010105:Oxygenases; C020066:dimethylaniline monooxygenase (N-oxide forming)