Saccharomyces cerevisiae strain 905 reduces the translocation of Salmonella enterica serotype Typhimurium and stimulates the immune system in gnotobiotic and conventional mice.
Previous results in the laboratory of the authors showed that Saccharomyces cerevisiae strain 905, isolated during 'cachaça' production, was able to colonize and survive in the gastrointestinal tract of germ-free and conventional mice, and to protect these animals against oral challenge with Salmonella enterica serotype Typhimurium or Clostridium difficile. In the present work, the effects of S. cerevisiae 905 on the translocation of Salm. Typhimurium (mesenteric lymph nodes, Peyer's patches, spleen, liver) as well as on the immune system (number of Küpffer cells, immunoglobulin production, clearance of Escherichia coli B41) were evaluated in gnotobiotic and/or conventional mice. The treatment with the yeast reduced significantly the translocation of Salm. Typhimurium to liver in gnotobiotic animals and to all the organs tested in conventional mice. The number of Küpffer cells per 100 hepatocytes in liver was significantly higher (P<0.05) in yeast mono-associated mice (52.9+/-15.7) than in germ-free controls (38.1+/-9.0). Probably as a consequence, clearance of E. coli B41 from the bloodstream was more efficient in yeast mono-associated animals when compared to germ-free mice. Higher levels (P<0.05) of secretory IgA in intestinal content and of IgA and IgM in serum were observed in yeast mono-associated mice when compared to germ-free group. Concluding, the protection against pathogenic bacteria observed in a previous study was probably due to a modulation of both local and systemic immunity of mice treated with S. cerevisiae 905.
Martins
Flaviano S
FS
Centro de Desenvolvimento da Tecnologia Nuclear/Comissão Nacional de Energia Nuclear (CDTN/CNEN), Belo Horizonte, MG, Brazil.|Departamento de Pediatria, Faculdade de Medicina, Universidade Federal de Minas Gerais, C.P. 486, 30161-970, Belo Horizonte, MG, Brazil.|Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, C.P. 486, 30161-970, Belo Horizonte, MG, Brazil.
Rodrigues
Ana Cristina P
ACP
Faculdade de Medicina, UNIFENAS, Belo Horizonte, MG, Brazil.
Tiago
Fabiana C P
FCP
Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, C.P. 486, 30161-970, Belo Horizonte, MG, Brazil.
Penna
Francisco J
FJ
Departamento de Pediatria, Faculdade de Medicina, Universidade Federal de Minas Gerais, C.P. 486, 30161-970, Belo Horizonte, MG, Brazil.
Rosa
Carlos A
CA
Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, C.P. 486, 30161-970, Belo Horizonte, MG, Brazil.
Arantes
Rosa M E
RME
Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, C.P. 486, 30161-970, Belo Horizonte, MG, Brazil.
Nardi
Regina M D
RMD
Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, C.P. 486, 30161-970, Belo Horizonte, MG, Brazil.
Neves
Maria J
MJ
Centro de Desenvolvimento da Tecnologia Nuclear/Comissão Nacional de Energia Nuclear (CDTN/CNEN), Belo Horizonte, MG, Brazil.
Nicoli
Jacques R
JR
Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, C.P. 486, 30161-970, Belo Horizonte, MG, Brazil.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000097:blood; D016470:Bacteremia / Q000276:immunology; D018988:Bacterial Translocation; D004195:Disease Models, Animal; D004926:Escherichia coli; D004927:Escherichia coli Infections / Q000276:immunology; D005243:Feces / Q000737:chemistry; D005856:Germ-Free Life; D007070:Immunoglobulin A / Q000097:blood; D007071:Immunoglobulin A, Secretory / Q000032:analysis; D007075:Immunoglobulin M / Q000097:blood; D007728:Kupffer Cells; D008099:Liver / Q000382:microbiology / Q000473:pathology; D008198:Lymph Nodes / Q000382:microbiology; D051379:Mice; D010581:Peyer's Patches / Q000382:microbiology; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D012481:Salmonella Infections, Animal / Q000276:immunology* / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000276:immunology* / Q000472:pathogenicity*; D013154:Spleen / Q000382:microbiology
D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory; D007075:Immunoglobulin M