Proteomic analyses of songbird (Zebra finch; Taeniopygia guttata) retina.
Proteomic analyses of male songbird (Zebra finch; Taeniopygia guttata; ZF) retina were performed resulting in identification of 129 proteins. Comparison of T. guttata retinal proteome with that of chicken found proteins detected in both retinas. Immunohistochemical analyses of T. guttata retinal sections and Western analyses of total retinal protein extract were performed confirming presence of select bona fide retinal proteins. Results demonstrate the utility of one-dimensional gel fractionation for mass spectrometry and will be useful for future proteomic comparison of songbird retina and brain tissues in different behavioral and pharmacological studies.
Sloley
Stephanie
S
Bascom Palmer Eye Institute, University of Miami, Miami, Florida, 33136, USA.
Smith
Shannon
S
Algeciras
Mabel
M
Cavett
Valerie
V
Busby
Jennifer A Caldwell
JA
London
Sarah
S
Clayton
David F
DF
Bhattacharya
Sanjoy K
SK
D000818:Animals; D001717:Birds / Q000235:genetics*; D015153:Blotting, Western; D002645:Chickens; D008297:Male; D013058:Mass Spectrometry; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D012160:Retina / Q000737:chemistry*
D011506:Proteins