title: Uncovering a link between a plastid translocon component and rhomboid proteases using yeast mitochondria-based assays.
abstract: Rhomboid proteases are present in bacteria, insects, yeasts, parasites, mammals and plants. These proteases are part of the regulated intramembrane proteolysis mechanism for controlling processes such as development, stress response, lipid metabolism and mitochondrial membrane remodeling. Specific rhomboid protease substrates linked to these processes have been identified from insects to mammals, but not for plants. Identification of a link is a key step for elucidating the role of each rhomboid protease. Here, using a yeast mitochondria-based approach, we report evidence of a potential link between a plastid translocon component and organellar rhomboid proteases. This identification expands the types of processes involving regulated intramembrane proteolysis potentially to include at least one aspect of plastid protein transport.
authors.affiliation: Department of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6.
authors.forename: Katherine
authors.initials: K
authors.lastname: Karakasis
authors.forename: Darcie
authors.initials: D
authors.lastname: Taylor
authors.forename: Kenton
authors.initials: K
authors.lastname: Ko
mesh_terms: D017360:Arabidopsis / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D018832:Molecular Chaperones / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism*; D018087:Plastids / Q000378:metabolism*; D012278:Ricinus / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D008565:Membrane Proteins; D018832:Molecular Chaperones; C476397:Tic40 protein, Arabidopsis; D010447:Peptide Hydrolases