The role of root damage in the chelate-enhanced accumulation of lead by Indian mustard plants.
In the present study, increasing ethylenediaminetetraacetic acid (EDTA) concentration from 0 to 0.5 mmol L(-1) resulted in progressive increases in root elongation and in shoot and root dry matter (DM) of Indian mustard seedlings (Brassica juncea. L.) exposed at 0.5 mmol L(-1) of lead (Pb). The highest concentration of Pb in the shoots of Indian mustard reached 1140 mg kg(-1) dry weight (DW) in the treatment with 0.5 mmol L(-1) of Pb + 0.25 mmol L(-1) of EDTA. A significantly positive correlation was found between the concentrations of Pb and EDTA in the shoots of mustard. Roots were pretreated with an MC (methanol:trichloromethane) solution, 0.1 mol L(-1) of HCl, and 65 degrees C hot water. The plants were then exposed to 0.5 mmol L(-1) of Pb + 3 mmol L(-1) of EDTA in solution for 2 d. The pretreatments with MC, HCl, and hot water all increased the concentration of Pb in shoots by 14-, 7-, and 15-fold, respectively, compared with the shoots that had not been pretreated. Therefore, some physiological damage to roots would be useful to enhance the uptake of metal by plants and to minimize the application of doses of chelates in the practical operation of chelate-assisted phytoremediation.
Luo
Chunling
C
College of Life Sciences, Nanjing Agricultural University, Nanjing, China.
Shen
Zhenguo
Z
Li
Xiangdong
X
Baker
Alan J M
AJ
D001673:Biodegradation, Environmental; D002614:Chelating Agents / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste / Q000517:prevention & control; D007854:Lead / Q000737:chemistry / Q000493:pharmacokinetics*; D009149:Mustard Plant / Q000254:growth & development / Q000378:metabolism*; D018517:Plant Roots; D018520:Plant Shoots; D012989:Soil Pollutants / Q000737:chemistry / Q000493:pharmacokinetics*
D002614:Chelating Agents; D007220:Industrial Waste; D012989:Soil Pollutants; D007854:Lead; D004492:Edetic Acid