Comparison of the effects of acylation and amidation on the antimicrobial and antiviral properties of lactoferrin.
OBJECTIVE
To compare amidation and acylation of lactoferrin (LF) from bovine milk, as a means of enhancing its antimicrobial and antiviral properties.


RESULTS
LF was chemically modified by amidation with a 1-ethyl-3-[3-(dimethylamino) propyl] carbodiimide (EDC) in the presence of ammonium ions or by acylation with either succinic or acetic anhydride. In the test systems used, amidation substantially enhanced the activity of LF against Pseudomonas fluorescens in comparison with native LF. However, increasing the net negative charge of LF by acylation had no effect on the activity of LF against P. fluorescens, and abrogated the antimicrobial activity of LF against Bacillus subtilis and Saccharomyces cerevisiae. Increasing the net negative charges of LF by acylation eliminated its antimicrobial and antiviral effects against poliovirus and feline calicivirus (nonenveloped viruses).


CONCLUSIONS
The addition of positive charges to LF via amidation enhanced antimicrobial properties in contrast to increasing the negative charges by acylation, which abolished both the antimicrobial and antiviral properties of LF.


CONCLUSIONS
The effects of charge alteration of LF determined in this study provides a basis for further development of LF formulations with enhanced antimicrobial effectiveness for use in food process hygiene, veterinary and health-care applications.
Pan
Y
Y
School of Agriculture and Food Systems, The University of Melbourne, Werribee, Victoria, Australia.
Wan
J
J
Roginski
H
H
Lee
A
A
Shiell
B
B
Michalski
W P
WP
Coventry
M J
MJ
D000215:Acylation; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D002417:Cattle; D007781:Lactoferrin / Q000737:chemistry* / Q000494:pharmacology*; D008892:Milk / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D014780:Viruses / Q000187:drug effects
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D000998:Antiviral Agents; D007781:Lactoferrin