[Differential analysis of two-dimensional gel electrophoresis profiles of spermatozoal protein in human normal semen and idiopathic asthenospermia].
OBJECTIVE
To evaluate the application of two-dimensional electrophoresis in the research of differentially expressed proteins in human spermatozoa.


METHODS
Two-dimensional gel electrophoresis was performed on 4 sperm samples from normal healthy men and another 4 from asthenospermia patients. After silver staining, the differential expression proteins were analyzed by PDQuest 2D analysis software.


RESULTS
Seven differential protein spots were identified, 2 expressed highly in the asthenospermia sperm but lowly in the normal spermatozoa, while the other 5 expressed just the opposite way.


CONCLUSIONS
The protein profiles of differential expression between the normal spermatozoa and idiopathic asthenospermia were established and some differential proteins were found. The data obtained in this study may help prepare the ground for further studies on the isolation and identification of differentially expressed proteins in human asthenospermia sperm.
Shen
Shu-lin
SL
Department of Urology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China. shenforest@163.com
Luo
Yong
Y
Ning
Liang
L
He
Da-lin
DL
D016022:Case-Control Studies; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008297:Male; D009845:Oligospermia / Q000378:metabolism*; D029589:Seminal Plasma Proteins / Q000032:analysis / Q000096:biosynthesis*; D013094:Spermatozoa / Q000737:chemistry
D029589:Seminal Plasma Proteins