Participation of nitric oxide reductase in survival of Pseudomonas aeruginosa in LPS-activated macrophages.
Nitric oxide (NO) plays a crucial role in the antimicrobial activity of host defense systems. We investigated the function of Pseudomonas aeruginosa NO reductase as a detoxifying enzyme in phagocytes. We found that the growth of the NO reductase-deficient mutant of P. aeruginosa under a microaerobic condition was inhibited by the exogenous NO. Furthermore, the intracellular survival assay within the NO-producing RAW 264.7 macrophages revealed that the wild-type strain survived longer than the NO reductase-deficient mutant. These results suggest that the P. aeruginosa NO reductase may contribute to the intracellular survival by acting as a counter component against the host's defense systems.
Kakishima
Kohei
K
Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa, Ishikawa 920-1192, Japan.
Shiratsuchi
Akiko
A
Taoka
Azuma
A
Nakanishi
Yoshinobu
Y
Fukumori
Yoshihiro
Y
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects* / Q000201:enzymology; D051379:Mice; D009569:Nitric Oxide / Q000494:pharmacology; D010088:Oxidoreductases / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development
D017931:DNA Primers; D008070:Lipopolysaccharides; D009569:Nitric Oxide; D010088:Oxidoreductases; C034747:nitric-oxide reductase