A comprehensive analysis of allelic methylation status of CpG islands on human chromosome 11q: comparison with chromosome 21q.
It was generally believed that autosomal CpG islands (CGIs) escape methylation. However, our comprehensive analysis of allelic methylation status of 149 CGIs on human chromosome 21q revealed that a sizable fraction of them are methylated on both alleles even in normal blood cells. Here, we performed a similar analysis of 656 CGIs on chromosome 11q, which is gene-rich in contrast with 21q. The results indicate that 11q contains less methylated CGIs, especially those with tandem repeats and those in the coding or 3'-untranslated regions (UTRs), than 21q. Thus, methylation status of CGIs may substantially differ from one chromosome to another.
Yamada
Yoichi
Y
Department of Information and Systems Engineering, Faculty of Engineering, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
Shirakawa
Tomoyo
T
Taylor
Todd D
TD
Okamura
Kohji
K
Soejima
Hidenobu
H
Uchiyama
Michiko
M
Iwasaka
Tsuyoshi
T
Mukai
Tsunehiro
T
Muramoto
Ken-Ichiro
K
Sakaki
Yoshiyuki
Y
Ito
Takashi
T
D001483:Base Sequence; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics*; D018899:CpG Islands / Q000235:genetics*; D019175:DNA Methylation; D017931:DNA Primers; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D017931:DNA Primers