Organotypic slice culture of the hypothalamic paraventricular nucleus of rat.
Organotypic slice cultures have been developed as an alternative to acute brain slices because the neuronal viability and synaptic connectivity in these cultures can be preserved well for a prolonged period of time. This study evaluated a stationary organotypic slice culture developed for the hypothalamic paraventricular nucleus (PVN) of rat. The results showed that the slice cultures maintain the typical shape of the nucleus, the immunocytochemical signals for oxytocin, vasopressin, and corticotropin-releasing hormone, and the electrophysiological properties of PVN neurons for up to 3 weeks in vitro. The PVN neurons in the culture expressed the green fluorescent protein gene that had been delivered by the adenoviral vectors. The results indicate that the cultured slices preserve the properties of the PVN neurons, and can be used in longterm studies on these neurons in vitro.
Cho
Eun Seong
ES
Laboratory of Veterinary Pharmacology, BK21 Program for Veterinary Science and College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
Lee
So Yeong
SY
Park
Jae Yong
JY
Hong
Seong Geun
SG
Ryu
Pan Dong
PD
D000256:Adenoviridae; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism; D004594:Electrophysiology; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007150:Immunohistochemistry; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D010078:Oxazines; D010121:Oxytocin / Q000378:metabolism; D010286:Paraventricular Hypothalamic Nucleus / Q000033:anatomy & histology* / Q000166:cytology / Q000378:metabolism; D051381:Rats; D014667:Vasopressins / Q000378:metabolism
D010078:Oxazines; D014667:Vasopressins; D049452:Green Fluorescent Proteins; C008619:Nile Blue; D010121:Oxytocin; D003346:Corticotropin-Releasing Hormone