Demethylation of trimethylated histone H3 Lys4 in vivo by JARID1 JmjC proteins.
Histone H3 Lys4 trimethylation (H3-K4me3) is a conserved mark of actively transcribed chromatin. Using a conditional mutant of the yeast H3-K4 methyltransferase, Set1p, we demonstrate rapid turnover of H3-K4me3 and H3-K4me2 in vivo and show this process requires Yjr119Cp, of the JARID1 family of JmjC proteins. Ectopic overexpression of mouse Jarid1B, a Yjr119Cp homolog, greatly diminished H3-K4me3 and H3-K4me2 in HeLa cells, suggesting these proteins function as K4 demethylases in vivo.
Seward
David J
DJ
Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, University of Colorado Health Sciences Center, MS8101, P.O. Box 6511, Aurora, Colorado 80045, USA.
Cubberley
Gabrielle
G
Kim
Soojin
S
Schonewald
Matt
M
Zhang
Lian
L
Tripet
Brian
B
Bentley
David L
DL
D000818:Animals; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005820:Genetic Testing; D006367:HeLa Cells; D011495:Histone-Lysine N-Methyltransferase / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D006801:Humans; D056484:Jumonji Domain-Containing Histone Demethylases; D008239:Lysine / Q000378:metabolism*; D008745:Methylation; D051379:Mice; D050505:Mutant Proteins / Q000378:metabolism; D009363:Neoplasm Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
D004268:DNA-Binding Proteins; D006657:Histones; D050505:Mutant Proteins; D009363:Neoplasm Proteins; D029701:Saccharomyces cerevisiae Proteins; C519119:JHD2 protein, S cerevisiae; D056484:Jumonji Domain-Containing Histone Demethylases; C485614:Kdm5b protein, mouse; D011495:Histone-Lysine N-Methyltransferase; D008239:Lysine