Radiation hybrid mapping and comparative sequence analysis of bovine RIG-I and MAVS genes.
Retinoic acid inducible gene I (RIG-I) and mitochondrial antiviral signaling (MAVS) proteins have recently been found to operate in a pathway for the detection and subsequent elimination of replicating viral genomes. Because of this innate immunity role, RIG-I and MAVS are candidates for studies of disease resistance. The objectives of this work were to (1) radiation hybrid (RH) map bovine RIG-I and MAVS and (2) perform comparative sequence analysis of partial genomic sequence from each gene. Using a bovine 5000(rad) RH panel, RIG-I was localized to BTA08 (LOD > 12) and MAVS was localized to BTA13 (LOD > 12). RIG-I exon 14 and partial MAVS exon five were sequenced in nine breeds and compared with available sequence from the Bovine Genome Project. RIG-I exon 14 and partial MAYS exon five were conserved in all samples examined. One T-A transversion SNP was found in intronic sequence downstream of RIG-I exon 14.
Cargill
Edward J
EJ
Department of Pathobiology, College of Veterinary Medicine, Texas A & M University, College Station, TX 77843-4467, USA.
Paetzold
Li
L
Womack
James E
JE
D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D019295:Computational Biology; D053487:DEAD-box RNA Helicases / Q000235:genetics*; D017931:DNA Primers; D024101:Mitochondrial Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D021101:Radiation Hybrid Mapping; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D024101:Mitochondrial Proteins; D053487:DEAD-box RNA Helicases