Sensitive and specific detection of Xanthomonas campestris pv. vesicatoria by PCR using pathovar-specific primers based on rhs family gene sequences.
The present study describes PCR assay to detect bacterial spot caused by Xanthomonas campestris pv. vesicatoria in pepper and tomato. One set of PCR primer was developed to amplify gene required for an rhs family gene homologous to rhsA, cell envelope biogenesis, outer membrane. Only a PCR product of a 517bp was produced in PCR reaction with the Xanthomonas campestris pv. vesicatoria (XCVF/XCVR) primer set. A specific, and highly sensitive and rapid PCR assay for the detection of X. campestris pv. vesicatoria was achieved. The protocol can be used as a reliable diagnostic tool for specific detection of X. campestris pv. vesicatoria in pepper or tomato.
Park
Dong Suk
DS
National Institute of Agricultural Biotechnology, Rural Development Administration, 441-707 Suwon, Republic of Korea. dspark@rda.go.kr
Shim
Jae Kyung
JK
Kim
Jung Sun
JS
Lim
Chun Keun
CK
Shrestha
Rosemary
R
Hahn
Jang Ho
JH
Kim
Hong Gi
HG
D001426:Bacterial Proteins / Q000235:genetics*; D002212:Capsicum / Q000382:microbiology; D017931:DNA Primers / Q000235:genetics*; D018551:Solanum lycopersicum / Q000382:microbiology; D005810:Multigene Family; D010935:Plant Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity; D016959:Xanthomonas campestris / Q000235:genetics / Q000302:isolation & purification*
D001426:Bacterial Proteins; D017931:DNA Primers