Expression of CD9 in frozen-thawed mouse oocytes: preliminary experience.
CD9 mRNA and protein expression levels in mouse slow frozen-rapid thawed oocytes were compared with those in fresh oocytes by using comparative quantitative real time reverse transcription-PCR and semiquantitative Western blot, respectively. The expression levels of both CD9 mRNA and protein in the frozen oocytes were significantly lower than those found in the fresh oocytes.
Wen
Yan
Y
Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford, California 94305-5317, USA. yanwen@stanford.edu
Quintero
Rudy
R
Chen
Bertha
B
Shu
Yimin
Y
Polan
Mary Lake
ML
Behr
Barry
B
D000818:Animals; D015703:Antigens, CD / Q000235:genetics* / Q000378:metabolism; D015925:Cryopreservation; D005260:Female; D005615:Freezing; D015870:Gene Expression; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009865:Oocytes / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism; D010865:Pilot Projects; D060245:Tetraspanin 29; D014018:Tissue Distribution
D015703:Antigens, CD; C554870:Cd9 protein, mouse; D008562:Membrane Glycoproteins; D060245:Tetraspanin 29