Hemodialysis shortens long in vitro closure times as measured by the PFA-100.
BACKGROUND
Hemorrhagic complications are commonly encountered in patients with end-stage renal disease (ESRD). Uremic patients show a bleeding diathesis mainly due to abnormalities in platelet function. There are several tests to detect and measure impairment of hemostasis in these patients, but none appear to be ideal. In recent years, the PFA-100 (platelet function analyzer) was introduced to measure primary, platelet-dependent hemostasis. In this study, the effect of hemodialysis on platelet function was evaluated using the PFA-100 in patients with ESRD.


METHODS
The study was performed on 45 patients with ESRD undergoing regular hemodialysis aged between 20-76 years (median: 54 years). Collagen/epinephrine (CEPI) and collagen/ADP (CADP) closure times were measured before and after the hemodialysis session using the PFA.


RESULTS
CEPI (normal range: 85-165 sec) was significantly shortened from 230+/-60 to 206+/-63 sec after hemodialysis (p<0.05). The CADP (normal range: 71-118 sec) was also shortened by hemodialysis from 177+/-69 to 169+/-71 sec (p>0.05). CEPI closure times of 10 (26%) in 38 patients with long CT returned to normal after hemodialysis. CADP closure times of 9 (25%) of 36 patients with long CT returned to normal after hemodialysis.


CONCLUSIONS
This study confirms the existence of a dysfunction of primary hemostasis in patients with ESRD, and hemodialysis has the ability to correct some part of the hemostatic disturbances. As a sensitive, specific, reproducible, easy to perform, and noninvasive test for platelet-related primary hemostasis, the PFA-100 system may become a useful tool for an overall evaluation of primary hemostasis in patients with ESRD.
Bilgin
Aynur Ugur
AU
Akdeniz University School Of Medicine, Department Of Hematology, Antalya, Turkey
Karadogan
Ihsan
I
Artac
Mehmet
M
Kizilors
Aytug
A
Bligin
Rasim
R
Undar
Levent
L
D000244:Adenosine Diphosphate / Q000378:metabolism; D000328:Adult; D000368:Aged; D001792:Blood Platelets / Q000166:cytology / Q000502:physiology*; D003094:Collagen / Q000378:metabolism; D004837:Epinephrine / Q000378:metabolism; D006400:Hematocrit; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D010976:Platelet Count; D010979:Platelet Function Tests; D006435:Renal Dialysis; D013997:Time Factors
D006454:Hemoglobins; D000244:Adenosine Diphosphate; D003094:Collagen; D004837:Epinephrine