Progenitor cells are responsible for formation primary epithelial cultures in the prostate epithelial model.
The adult stem cells (ASC) are supposed to regenerate epithelium. We hypothesized prostate epithelial CD133-positive ASC to be responsible for establishing the primary cell culture. The prostate epithelial stem cells were isolated using anti-CD133 microbeads in order to form different cell populations. The morphology of cultures developed from CD133(+) and CD133(-) prostate epithelial cells were compared with prostate epithelium cell culture obtained after simple isolation procedure. Four 8-week-old Wistar rats were used in the experiment and six cultures were obtained. Double CD133(+) and CD133(-) cultures from two rats were established after enzymatic digestion and positive selection by SuperMACS device, and two non-selected CD133(+)/CD133(-) cultures were developed by simple prostate epithelial cell isolation from two other rats. The epithelial nature was confirmed by anti-cytokeratine antibodies. It was observed that growth of the CD133(+)/CD133(-) and CD133(+)culture resembled epithelial-like prostate cell culture. It was not possible to establish epithelial-like culture from CD133(-) cell population. The primary epithelial cell culture collapsed in a few days after the CD133-positive ASC were removed. We concluded that the epithelial progenitor cells are responsible for establishing primary prostate epithelial cultures in vitro.
Drewa
Tomasz
T
Department of Tissue Engineering, Chair of Medical Biology, Nicolaus Copernicus University, Karlowicza 24, 85-092, Bydgoszcz, Poland. tomaszdrewa@wp.pl
Styczynski
Jan
J
D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D008297:Male; D008863:Microspheres; D023421:Models, Animal; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000378:metabolism*; D051381:Rats; D014407:Tumor Cells, Cultured
D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; D010455:Peptides; C000606025:Prom1 protein, rat