title: First Serbian isolates of bovine herpesvirus 4 (BoHV-4) from a herd with a history of postpartum metritis.
abstract: Two farms in the Belgrade area have experienced serious problems with postpartal metritis. Serological examination of BoHV-4 infection was done using ELISA test and vaginal swabs were used for virus isolation. Average seroprevalence of BoHV-4 in these farms was 84.37%. BoHV-4 isolation was successful from three vaginal swabs on the MDBK cell line. Rising values of BoHV-4 antibodies were recorded in nine of ten cows with clinical signs of postpartal metritis. PCR and restriction analysis were used for better characterisation and isolate classification. Two isolates showed similarity with MOVAR 33/63 virus type, but one differed in polyrepetitive and other parts of DNA. This was the first isolation and characterisation of BoHV4 from Serbian herds.
authors.affiliation: Department of Virology, The Institute of Veterinary Medicine of Serbia, Belgrade, Belgrade, Serbia. vetina@verat.net
authors.forename: Veljko M
authors.initials: VM
authors.lastname: Nikolin
authors.forename: Gaetano
authors.initials: G
authors.lastname: Donofrio
authors.forename: Bratislav
authors.initials: B
authors.lastname: Milosevic
authors.forename: Simone
authors.initials: S
authors.lastname: Taddei
authors.forename: Vladimir
authors.initials: V
authors.lastname: Radosavljevic
authors.forename: Vesna
authors.initials: V
authors.lastname: Milicevic
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000821:virology; D004196:Disease Outbreaks; D004716:Endometritis / Q000662:veterinary* / Q000821:virology; D005260:Female; D006566:Herpesviridae Infections / Q000662:veterinary* / Q000821:virology; D026021:Herpesvirus 4, Bovine / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D049590:Postpartum Period; D011247:Pregnancy; D015183:Restriction Mapping; D015022:Yugoslavia / Q000453:epidemiology
chemical_list: D000914:Antibodies, Viral