title: Linear motifs in the C-terminus of D. melanogaster cryptochrome.
abstract: The C-terminus of cryptochrome (CRY) regulates light responses in Drosophila. These include the light-dependent binding of Drosophila dCRY to the clock proteins PERIOD and TIMELESS in a yeast two-hybrid system, which we proved to be a convenient and reliable readout of the behavior of dCRY in vivo. In this study, we present a combination of in silico analysis and experimental validation in yeast, to identify novel functional motifs in the C-terminal region of dCRY. Our results suggest that linear motifs are present in this small region, which is a likely hotspot for molecular interactions.
authors.affiliation: Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, UK.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Hemsley
authors.forename: Gabriella M
authors.initials: GM
authors.lastname: Mazzotta
authors.forename: Moyra
authors.initials: M
authors.lastname: Mason
authors.forename: Stephane
authors.initials: S
authors.lastname: Dissel
authors.forename: Stefano
authors.initials: S
authors.lastname: Toppo
authors.forename: Mario A
authors.initials: MA
authors.lastname: Pagano
authors.forename: Federica
authors.initials: F
authors.lastname: Sandrelli
authors.forename: Flavio
authors.initials: F
authors.lastname: Meggio
authors.forename: Ezio
authors.initials: E
authors.lastname: Rosato
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Costa
authors.forename: Silvio C E
authors.initials: SC
authors.lastname: Tosatto
mesh_terms: D000818:Animals; D056931:Cryptochromes; D004331:Drosophila melanogaster; D015180:Electrophoresis, Gel, Two-Dimensional; D005420:Flavoproteins / Q000737:chemistry* / Q000235:genetics; D047468:Immunoprecipitation; D009154:Mutation; D010766:Phosphorylation; D011487:Protein Conformation; D020798:Two-Hybrid System Techniques
chemical_list: D056931:Cryptochromes; D005420:Flavoproteins