Anticlastogenic effect of redistilled cow's urine distillate in human peripheral lymphocytes challenged with manganese dioxide and hexavalent chromium.
OBJECTIVE
To study the anticlastogenic effect of redistilled cow's urine distillate (RCUD) in human peripheral lymphocytes (HLC) challenged with manganese dioxide and hexavalent chromium.


METHODS
The anticlastogenic activity of redistilled cow's urine distillate was studied in human polymorphonuclear leukocytes (HPNLs) and human peripheral lymphocytes in vitro challenged with manganese dioxide and hexavalent chromium as established genotoxicants and clastogens which could cause induction of DNA strand break, chromosomal aberration and micronucleus. Three different levels of RCUD: 1 microL/mL, 50 microL/mL and 100 microL/mL, were used in the study.


RESULTS
Manganese dioxide and hexavalent chromium caused statistically significant DNA strand break, chromosomal aberration and micronucleus formation, which could be protected by redistilled cow's urine distillate.


CONCLUSIONS
The redistilled cow's urine distillate posseses strong antigenotoxic and anticlastogenic properties against HPNLs and HLC treated with Cr+6 and MnO2. This property is mainly due to the antioxidants present in RCUD.
Dutta
Dipanwita
D
Environmental Biotechnology Division, National Environmental Engineering Research Institute, Nehru Marg, Nagpur--440020, INDIA.
Devi
S Saravana
SS
Krishnamurthi
K
K
Chakrabarti
T
T
D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology; D002417:Cattle / Q000652:urine*; D002478:Cells, Cultured; D002857:Chromium / Q000037:antagonists & inhibitors* / Q000633:toxicity; D004249:DNA Damage; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008214:Lymphocytes / Q000187:drug effects; D017895:Manganese Compounds / Q000037:antagonists & inhibitors*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity; D010087:Oxides / Q000037:antagonists & inhibitors* / Q000633:toxicity; D014556:Urine / Q000737:chemistry
D016587:Antimutagenic Agents; D000975:Antioxidants; D017895:Manganese Compounds; D009153:Mutagens; D010087:Oxides; D002857:Chromium; C074702:chromium hexavalent ion; C016552:manganese dioxide