title: Osteogenic growth peptide C-terminal pentapeptide [OGP(10-14)] acts on rat bone marrow mesenchymal stem cells to promote differentiation to osteoblasts and to inhibit differentiation to adipocytes.
abstract: Cumulative evidence indicates that bone marrow mesenchymal stem cells (MSCs) are multipotent cells capable of differentiating to osteogenic and adipogenic lineages when stimulated under appropriate conditions. Whether OGP(10-14) directly regulates the progenitor cells differentiating into osteoblasts or adipocytes remains unknown. In the present study, we investigated the roles of OGP(10-14) in differentiation along these separate lineages using rat bone marrow MSCs. Our results showed that OGP(10-14) promoted osteogenic differentiation of the stem cells and concurrently inhibited adipocyte formation. OGP(10-14) increased alkaline phosphatase (ALP) activity and mineralized nodule formation, and stimulated osteoblast-specific mRNA expression of core-binding factor 1 (cbfa1). In contrast, OGP(10-14) decreased adipocyte numbers and inhibited adipocyte-specific mRNA expression of peroxisome proliferator-activated receptor-gamma 2 (PPARgamma2). These observations suggest that commitment of MSCs into osteogenic or adipogenic lineages is regulated by OGP(10-14).
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Life Science, Lanzhou University, Lanzhou 730000, PR China.
authors.forename: Zhi-xin
authors.initials: ZX
authors.lastname: Chen
authors.forename: Min
authors.initials: M
authors.lastname: Chang
authors.forename: Ya-li
authors.initials: YL
authors.lastname: Peng
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
authors.forename: Yu-rui
authors.initials: YR
authors.lastname: Zhan
authors.forename: Li-jing
authors.initials: LJ
authors.lastname: Wang
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics; D004723:Endorphins / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008858:Microscopy, Phase-Contrast; D041681:NIH 3T3 Cells; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; D004723:Endorphins; C050198:historphin; D000469:Alkaline Phosphatase