[Biodiversity of acidophiles in the sediment at an acid mine drainage site].
A sediment sample was collected at an acid mine drainage site in Anhui. The acidophiles composition and community structure in the sediment was studied with 16S rDNA clone library and denaturing gradient gel electrophoresis (DGGE). The results indicated that the acidophiles in the sediment were novel compared with the data in GenBank. The acidophiles affiliated with Acidobacteria, beta/gamma-Proteobacteria, delta-Proteobacteria, Nitrospira, Candidate Division TM7, low G + C Gram-positive. delta-Proteobacteria were major group in this acidic ecosystem. The research of aicdophiles community in sediment can help us develop high efficient method to treat acid mine drainage (AMD).
Hao
Chun-Bo
CB
Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. hao20709@yahoo.com.cn
Zhang
Hong-Xun
HX
Bai
Zhi-Hui
ZH
Zhang
Bao-Guo
BG
Zhang
Xu-Xiang
XX
D042763:Acidithiobacillus / Q000302:isolation & purification; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D019015:Geologic Sediments / Q000382:microbiology*; D007220:Industrial Waste / Q000032:analysis; D008906:Mining; D010802:Phylogeny; D020560:Proteobacteria / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013455:Sulfur; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000032:analysis
D007220:Industrial Waste; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; D013455:Sulfur