Multiplex specific PCR for identification of the genera Actinopolyspora and Streptomonospora, two groups of strictly halophilic filamentous actinomycetes.
The genera Actinopolyspora and Streptomonospora are two groups of extremely halophilic filamentous actinomycetes. Members of these two genera are isolated frequently, probably due to the high occurrence of these actinomycetes in the hypersaline soil environment. Although members of these genera can be identified by micromorphological criteria, the extensive chemotaxonomic characterization of each new isolates is a time-consuming task which cannot always be undertaken when handling large numbers of isolates as is the case in natural products screening programmes. In this work, the design of one set of genus-specific PCR primers which allows rapid detection of members of the genus Actinopolyspora by means of PCR amplification is presented. And we developed a multiplex PCR protocol for identification of the species of the genera Actinopolyspora and Streptomonospora, simultaneously.
Zhi
Xiao-Yang
XY
Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, People's Republic of China.
Yang
Ling-Ling
LL
Wu
Jin-Yuan
JY
Tang
Shu-Kun
SK
Li
Wen-Jun
WJ
D039903:Actinobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D017931:DNA Primers; D005800:Genes, Fungal; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers