title: Rapid screening and sensitive detection of NPM1 (nucleophosmin) exon 12 mutations in acute myeloid leukaemia.
abstract: Nucleophosmin mutations of exon 12 (NPM1 mutations) represent the most frequent molecular aberration that can be found in patients with acute myeloid leukaemia (AML) and can be detected in about 35% of AML patients. NPM1 mutations are characterised by four basepair insertions within the region corresponding to the C-terminus of the protein leading to its translocation out of the nucleus. Until now, more than 40 different subsets of mutations have been identified and about 90% of NPM1 mutations are represented by subtype A and B (78% versus 12%, respectively). So far, standard screening of NPM1 mutations using conventional polymerase chain reaction (PCR) followed by capillary electrophoresis is rather time consuming. We established a new method for rapid screening of NPM1 mutations using the fluorescence resonance energy transfer (FRET) principle. Furthermore, based on individual NPM1 mutations type A and B, we designed mutation specific primers to perform a highly sensitive PCR assay that can be applied for the detection of minimal residual disease (MRD). In summary, we demonstrate new methodological approaches for rapid screening of NPM1 mutations as well as for MRD analyses based on the most frequent NPM1 mutations.
authors.affiliation: Department of Internal Medicine II (Oncology and Haematology), Friedrich Schiller University, Jena, Germany. sebastian.scholl@med.uni-jena.de
authors.forename: S
authors.initials: S
authors.lastname: Scholl
authors.forename: L-O
authors.initials: LO
authors.lastname: Mügge
authors.forename: O
authors.initials: O
authors.lastname: Landt
authors.forename: I F
authors.initials: IF
authors.lastname: Loncarevic
authors.forename: C
authors.initials: C
authors.lastname: Kunert
authors.forename: J H
authors.initials: JH
authors.lastname: Clement
authors.forename: K
authors.initials: K
authors.lastname: Höffken
mesh_terms: D000208:Acute Disease; D017931:DNA Primers; D005091:Exons / Q000235:genetics*; D005820:Genetic Testing; D006801:Humans; D007951:Leukemia, Myeloid / Q000175:diagnosis* / Q000235:genetics*; D009154:Mutation / Q000235:genetics*; D018365:Neoplasm, Residual / Q000175:diagnosis / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics*; D000090243:Nucleophosmin; D010750:Phosphoproteins / Q000235:genetics; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D010750:Phosphoproteins; D000090243:Nucleophosmin