title: Appropriate suppression of Notch signaling by Mesp factors is essential for stripe pattern formation leading to segment boundary formation.
abstract: Mesp1 and Mesp2 are homologous transcription factors that are co-expressed in the anterior presomitic mesoderm (PSM) during mouse somitogenesis. The loss of Mesp2 alone in our conventional Mesp2-null mice results in the complete disruption of somitogenesis, including segment border formation, rostro-caudal patterning and epithelialization of somitic mesoderm. This has led us to interpret that Mesp2 is solely responsible for somitogenesis. Our novel Mesp2 knock-in alleles, however, exhibit a remarkable upregulation of Mesp1. Removal of the pgk-neo cassette from the new allele leads to localization of Mesp1 and several gene expression, and somite formation in the tail region. Moreover, a reduction in the gene dosage of Mesp1 by one copy disrupts somite formation, confirming the involvement of Mesp1 in the rescue events. Furthermore, we find that activated Notch1 knock-in significantly upregulates Mesp1 expression, even in the absence of a Notch signal mediator, Psen1. This indicates that the Psen1-independent effects of activated Notch1 are mostly attributable to the induction of Mesp1. However, we have also confirmed that Mesp2 enhances the expression of the Notch1 receptor in the anterior PSM. The activation and subsequent suppression of Notch signaling might thus be a crucial event for both stripe pattern formation and boundary formation.
authors.affiliation: Cellular and Molecular Toxicology Division, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagayaku, Tokyo 158-8501, Japan. yutak@nihs.go.jp
authors.forename: Yu
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Yukuto
authors.initials: Y
authors.lastname: Yasuhiko
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kitajima
authors.forename: Jun
authors.initials: J
authors.lastname: Kanno
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Saga
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D019521:Body Patterning; D004622:Embryo, Mammalian / Q000378:metabolism; D018628:Gene Dosage; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D008817:Mice, Mutant Strains; D053764:Presenilin-1 / Q000502:physiology; D051881:Receptor, Notch1 / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction; D019170:Somites / Q000502:physiology*; D015854:Up-Regulation
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D053764:Presenilin-1; D051881:Receptor, Notch1; C101538:Mesp1 protein, mouse; C107407:Mesp2 protein, mouse; C497762:Notch1 protein, mouse