Application of synthetic peptides in development of a serologic method for laboratory diagnosis of schistosomiasis mansoni.
The immunoreactivity of seven peptides synthesized from Schistosoma mansoni proteins, was evaluated by dot-blot and ELISA assays using two different sensitization methodologies. The best results were obtained on wells of the Costar 3590 microplates coated with peptides P1, P2, P3, P6, and P7 using conventional methodology. The signals increased considerably (p < 0.0003) on wells sensitized with P1 to P6 using alternative methodology. In contrast, the well coated with peptide P7 presented lower signal when compared with conventional methodology (p = 0.0019). These results, establish the basis for the application of synthetic peptides for laboratory diagnosis of schistosomiasis mansoni.
Oliveira
Edward José de
EJ
Laboratório de Imunologia Clínica, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Av. Prof. Lineu Prestes 580, 05508-900 São Paulo, SP, Brazil. edwardjo@usp.br
Kanamura
Hermínia Yohko
HY
Takei
Kioko
K
Hirata
Rosario Dominguez Crespo
RD
Nguyen
Nga Yen
NY
Hirata
Mário Hiroyuki
MH
D000818:Animals; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D015801:Helminth Proteins; D010455:Peptides; D012555:Schistosomiasis mansoni / Q000175:diagnosis*
D015801:Helminth Proteins; D010455:Peptides