Evaluation of the clastogenicity and anticlastogenicity of vitamin B6 in human lymphocyte cultures.
Insufficient intakes of many micronutrients found in fruits and vegetables, such as folic acid, vitamins C and B6 may lead to DNA damage, cancer, and degenerative disease. The investigation of dietary antioxidants is a field of great interest for elucidating mechanisms of mutagenesis/carcinogenesis. The present study was undertaken to investigate the effects of vitamin B6 on the induction of chromosomal aberrations in cultured human lymphocytes and to examine the possible anticlastogenic effect of this vitamin on chromosomal damage induced by the antitumor drug doxorubicin. The results showed that when the cultures treated with vitamin B6 were compared with the untreated control in terms of total chromosomal damage and abnormal metaphases, pre- and simultaneous treatment with this vitamin showed no significant differences. In the post-treatment, average and above average concentrations of vitamin B6 alone showed a clastogenic effect. In the simultaneous protocol, this vitamin (15, 90 and 120 microg/mL) was effective in inhibiting chromosomal aberrations induced by doxorubicin (p<0.05), with a reduction of 33.1% with the highest concentration tested. However, in the post-treatment, the associations of vitamin B6 and doxorubicin exerted a more evident clastogenic effect than that observed in the cultures exposed only to the antitumor drug. In the present investigation, the inability of vitamin B6 to decrease chromosomal damage induced by doxorubicin in the pre- and post-treatments could be justified by the instability of this vitamin as a free radical scavenger. In conclusion, the results from this study confirmed that vitamin B6 is protective against chromosomal damage induced by doxorubicin in cultured human lymphocytes, but that the effects depend on concentration and form of treatment.
Takeuchi
Paula Lumy
PL
Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto (SP), Brazil. paulinha_lumyt@hotmail.com
Antunes
Lusânia Maria Greggi
LM
Takahashi
Catarina Satie
CS
D016587:Antimutagenic Agents; D002478:Cells, Cultured; D002842:Chromatids / Q000187:drug effects; D002869:Chromosome Aberrations / Q000187:drug effects; D004317:Doxorubicin / Q000633:toxicity; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008677:Metaphase / Q000187:drug effects; D008940:Mitotic Index; D009153:Mutagens; D025101:Vitamin B 6 / Q000494:pharmacology* / Q000633:toxicity*
D016587:Antimutagenic Agents; D009153:Mutagens; D004317:Doxorubicin; D025101:Vitamin B 6