Moderate ferulate and diferulate levels do not impede maize cell wall degradation by human intestinal microbiota.
The degradation of plant fiber by human gut microbiota could be restricted by xylan substitution and cross-linking by ferulate and diferulates, for example, by hindering the association of enzymes such as xylanases with their substrates. To test the influence of feruloylation on cell wall degradability by human intestinal microbiota, nonlignified primary cell walls from maize cell suspensions, containing various degrees of ferulate substitution and diferulate cross-linking, were incubated in nylon bags in vitro with human fecal microbiota. Degradation rates were determined gravimetrically, and the cell walls were analyzed for carbohydrates, ferulate monomers, dehydrodiferulates, dehydrotriferulates, and other minor phenolic constituents. Shifting cell wall concentrations of total ferulates from 1.5 to 15.8 mg/g and those of diferulates from 0.8 to 2.6 mg/g did not alter the release of carbohydrates or the overall degradation of cell walls. After 24 h of fermentation, the degradation of xylans and pectins exceeded 90%, whereas cellulose remained undegraded. The results indicate that low to moderate levels of ferulates and diferulates do not interfere with hydrolysis of nonlignified cell walls by human gut microbiota.
Funk
Carola
C
Institute of Biochemistry and Food Chemistry, Department of Food Chemistry, University of Hamburg, Grindelallee 117, 20146 Hamburg, Germany.
Braune
Annett
A
Grabber
John H
JH
Steinhart
Hans
H
Bunzel
Mirko
M
D001419:Bacteria / Q000378:metabolism*; D002473:Cell Wall / Q000737:chemistry* / Q000378:metabolism; D003373:Coumaric Acids / Q000032:analysis* / Q000737:chemistry; D003432:Cross-Linking Reagents; D005285:Fermentation; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D003313:Zea mays / Q000378:metabolism*
D003373:Coumaric Acids; D003432:Cross-Linking Reagents; C516502:diferulic acid; C004999:ferulic acid