title: Quantitative technologies establish a novel microRNA profile of chronic lymphocytic leukemia.
abstract: MicroRNAs (miRNAs) are a novel class of small noncoding RNAs that modulate the expression of genes at the posttranscriptional level. These small molecules have been shown to be involved in cancer, apoptosis, and cell metabolism. In the present study we provide an informative profile of the expression of miRNAs in primary chronic lymphocytic leukemia (CLL) cells using 2 independent and quantitative methods: miRNA cloning and quantitative real-time-polymerase chain reaction (qRT-PCR) of mature miRNAs. Both approaches show that miR-21 and miR-155 are dramatically overexpressed in patients with CLL, although the corresponding genomic loci are not amplified. miR-150 and miR-92 are also significantly deregulated in patients with CLL. In addition, we detected a marked miR-15a and miR-16 decrease in about 11% of cases. Finally, we identified a set of miRNAs whose expression correlates with biologic parameters of prognostic relevance, particularly with the mutational status of the IgV(H) genes. In summary, the results of this study offer for the first time a comprehensive and quantitative profile of miRNA expression in CLL and their healthy counterpart, suggesting that miRNAs could play a primary role in the disease itself.
authors.affiliation: Dipartimento di Biotecnologie Cellulari ed Ematologia, Sezione di Genetica Molecolare, Università di Roma La Sapienza, Rome, Italy.
authors.forename: Valerio
authors.initials: V
authors.lastname: Fulci
authors.forename: Sabina
authors.initials: S
authors.lastname: Chiaretti
authors.forename: Marina
authors.initials: M
authors.lastname: Goldoni
authors.forename: Gianluca
authors.initials: G
authors.lastname: Azzalin
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Carucci
authors.forename: Simona
authors.initials: S
authors.lastname: Tavolaro
authors.forename: Leandro
authors.initials: L
authors.lastname: Castellano
authors.forename: Armando
authors.initials: A
authors.lastname: Magrelli
authors.forename: Franca
authors.initials: F
authors.lastname: Citarella
authors.forename: Monica
authors.initials: M
authors.lastname: Messina
authors.forename: Roberta
authors.initials: R
authors.lastname: Maggio
authors.forename: Nadia
authors.initials: N
authors.lastname: Peragine
authors.forename: Simona
authors.initials: S
authors.lastname: Santangelo
authors.forename: Francesca Romana
authors.initials: FR
authors.lastname: Mauro
authors.forename: Pablo
authors.initials: P
authors.lastname: Landgraf
authors.forename: Thomas
authors.initials: T
authors.lastname: Tuschl
authors.forename: David B
authors.initials: DB
authors.lastname: Weir
authors.forename: Minchen
authors.initials: M
authors.lastname: Chien
authors.forename: James J
authors.initials: JJ
authors.lastname: Russo
authors.forename: Jingyue
authors.initials: J
authors.lastname: Ju
authors.forename: Robert
authors.initials: R
authors.lastname: Sheridan
authors.forename: Chris
authors.initials: C
authors.lastname: Sander
authors.forename: Mihaela
authors.initials: M
authors.lastname: Zavolan
authors.forename: Anna
authors.initials: A
authors.lastname: Guarini
authors.forename: Robin
authors.initials: R
authors.lastname: Foà
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Macino
mesh_terms: D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D020869:Gene Expression Profiling; D005821:Genetic Techniques; D016678:Genome; D006801:Humans; D007136:Immunoglobulins / Q000737:chemistry; D017404:In Situ Hybridization, Fluorescence; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000175:diagnosis* / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D012323:RNA Processing, Post-Transcriptional; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051746:ZAP-70 Protein-Tyrosine Kinase / Q000378:metabolism
chemical_list: D007136:Immunoglobulins; D035683:MicroRNAs; D051746:ZAP-70 Protein-Tyrosine Kinase