Optimization of culture of Leptospira from humans with leptospirosis.
A prospective study of 989 patients with acute febrile illness was performed in northeast Thailand to define the yield of Leptospira from four different types of blood sample. Based on a comparison of the yields from whole blood, surface plasma, deposit from spun plasma, and clotted blood samples from 80 patients with culture-proven leptospirosis, we suggest a sampling strategy in which culture is performed using whole blood and deposit from spun plasma.
Wuthiekanun
Vanaporn
V
Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Road, Bangkok 10400, Thailand. lek@tropmedres.ac
Chierakul
Wirongrong
W
Limmathurotsakul
Direk
D
Smythe
Lee D
LD
Symonds
Meegan L
ML
Dohnt
Michael F
MF
Slack
Andrew T
AT
Limpaiboon
Roongreung
R
Suputtamongkol
Yupin
Y
White
Nicholas J
NJ
Day
Nicholas P J
NP
Peacock
Sharon J
SJ
D001431:Bacteriological Techniques; D001769:Blood / Q000382:microbiology*; D006801:Humans; D007919:Leptospira / Q000254:growth & development* / Q000302:isolation & purification*; D007922:Leptospirosis / Q000382:microbiology*; D012680:Sensitivity and Specificity; D013048:Specimen Handling; D013785:Thailand