[Construction of tissue engineering bone with deproteinized bone as scaffold in vitro: experiment with rabbits].
OBJECTIVE
To construct a tissue engineering bone with deproteinized bone (DPB) as scaffold in vitro.


METHODS
The upper end of tibia, lower end of humerus, and cancellous bone of upper end of humerus of a New Zealand rabbit were taken out to make bars of DPB. The physical and chemical characteristics and the protein content therein were tested. The morphological characteristics were observed by light, Inverted phase contrast, and stereo microscopes. Osteoblasts (OBs) were obtained from the cranial bone of a fetal New Zealand rabbit and inoculated on the scaffold of DBP. The DBP scaffolds inoculated with OBs were cultured. Enzyme digestion method was used to observe the growth of OBs. Inverted phase microscopy was used to observe the morphology and adhesion of OBs. The growth of OBs on the DPB 1 and 7 days after culture was observed by scanning electron microscopy.


RESULTS
The protein content in the DPB was trivial. The OBs attached to the outside and inside surfaces and grew well.


CONCLUSIONS
The compound body of DPB and OBs show certain biological characteristics of tissue engineering bone, thus laying an experimental foundation for further observation of its revascularization and bone formation in vivo and for the repair of large bone defect.
Fan
Wei
W
Department of Orthopedics, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
An
Hong
H
Jiang
Dian-ming
DM
Cao
Ben-zhen
BZ
D000818:Animals; D018786:Bone Substitutes / Q000378:metabolism; D001842:Bone and Bones / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D008855:Microscopy, Electron, Scanning; D008858:Microscopy, Phase-Contrast; D010006:Osteoblasts / Q000166:cytology* / Q000648:ultrastructure; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
D018786:Bone Substitutes