title: Sck1 activator coordinates glucose transport and glycolysis and is controlled by Rag8 casein kinase I in Kluyveromyces lactis.
abstract: Casein kinases I (CKI) are ubiquitous in eukaryotic cells and are crucial factors for nutrient-signalling pathways in yeasts. In Kluyveromyces lactis, the KlRgt1 repressor represses the expression of the glucose transporter RAG1 gene in absence of glucose, but in response to glucose availability, Rag8 CKI cooperates with the Rag4 glucose sensor to inactivate KlRgt1. The SCK1 gene, a rag8 mutation suppressor, encodes a bHLH activator required for maximal expression of the RAG1 and glycolytic genes in the presence of glucose. We investigated further the function of Sck1 and its relationship to Rag8. We demonstrated that Sck1 regulates the RAG1 and glycolytic genes by directly binding to their promoter. We also found that SCK1 gene expression was induced by glucose and repressed by KlRgt1. In addition, we showed that (i) Sck1 was phosphorylated in vivo, (ii) Sck1 was phosphorylated in vitro by Rag8, and (iii) Sck1 was rapidly degraded in a rag8 mutant. We therefore suggest that Sck1 coordinates glucose import and glycolysis in K. lactis and that Rag8 controls this transcription factor by transcriptional and post-translational regulations.
authors.affiliation: Institut Pasteur, Paris, France.
authors.forename: Helen
authors.initials: H
authors.lastname: Neil
authors.forename: Martina
authors.initials: M
authors.lastname: Hnatova
authors.forename: Micheline
authors.initials: M
authors.lastname: Wésolowski-Louvel
authors.forename: Adriana
authors.initials: A
authors.lastname: Rycovska
authors.forename: Marc
authors.initials: M
authors.lastname: Lemaire
mesh_terms: D000595:Amino Acid Sequence; D019677:Artificial Gene Fusion; D047389:Casein Kinase I / Q000378:metabolism*; D004271:DNA, Fungal / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D005656:Fungal Proteins / Q000502:physiology; D015966:Gene Expression Regulation, Fungal; D017930:Genes, Reporter; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis / Q000235:genetics; D007716:Kluyveromyces / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D016415:Sequence Alignment; D015534:Trans-Activators / Q000378:metabolism*; D001616:beta-Galactosidase / Q000032:analysis / Q000235:genetics
chemical_list: D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D015534:Trans-Activators; D047389:Casein Kinase I; D001616:beta-Galactosidase; D005947:Glucose