Effects of taxol treatment on the microtubular system and mitochondria of Tetrahymena.
Complex investigation was done using immunocytochemical confocal microscopy, electron microscopy and flow cytometry on the effect of taxol to the microtubular arrangement and dynamics. The most interesting phenomenon was the rapid disappearance of transversal microtubule bands, while longitudinal microtubule bands remained and were submitted to the known effects of taxol. There was a broad variation in mitochondrial effect, some of them remained normal, while others swollen, desintegrated and their tubules disoriented. Treatment with 50 nM taxol significantly reduced the binding of anti alpha-tubulin antibody and a lesser degree anti-acetylated tubulin antibody. The difference between the transversal and longitudinal microtubules is emphasized by the results and the paper discusses the possibilities of indirect effects of taxol to the transversal microtubules (tubulin-GTP interaction, faster turnover, mitochondrial interaction). Polyglutamylation of tubulin has not a role in this difference.
Kovács
P
P
Department of Genetics, Cell- and Immunobiology, Semmelweis University, Nagyvárad tér 4, POB 370, H-1445 Budapest, Hungary.
Csaba
G
G
Pállinger
Eva
E
Czaker
Renate
R
D000107:Acetylation; D000818:Animals; D005434:Flow Cytometry; D018613:Microscopy, Confocal; D008870:Microtubules / Q000187:drug effects*; D008928:Mitochondria / Q000187:drug effects*; D017239:Paclitaxel / Q000494:pharmacology*; D010455:Peptides / Q000378:metabolism; D013769:Tetrahymena pyriformis / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D014404:Tubulin / Q000378:metabolism
D010455:Peptides; D014404:Tubulin; C097188:polyglutamine; D017239:Paclitaxel