One-pot synthesis, purification, and formulation of bionanoparticle-CpG oligodeoxynucleotide hepatitis B surface antigen conjugate vaccine via tangential flow filtration.
The synthesis and characterization of a Hepatitis B virus vaccine (HBsIC-ISS) candidate composed of Hepatitis B surface antigen (HBsAg) bionanoparticles conjugated to multiple copies of immunostimulatory sequence oligodeoxynucleotides is presented. An efficient tangential flow filtration (TFF) method has been developed to purify the conjugated bionanoparticles from the excess conjugation reagents. The TFF technique presented can serve as a rapid and convenient alternative to current methods like ultracentrifugation for the separation of excess small molecule/polymeric conjugation reagents from chemically modified viruses and other viruslike particles.
Raja
Krishnaswami
K
Department of Chemistry, The Institute for Macromolecular Assemblies, Center for Engineered Polymeric Materials, City University of New York at the College of Staten Island, 2800 Victory Boulevard, Staten Island, New York 10314, USA. raja@mail.csi.cuny.edu
McDonald
Ross
R
Tuck
Stephen
S
Rodriguez
Roberto
R
Milley
Bob
B
Traquina
Paula
P
D006514:Hepatitis B Surface Antigens / Q000737:chemistry* / Q000302:isolation & purification; D017325:Hepatitis B Vaccines / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification; D049329:Nanostructures / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification; D014462:Ultrafiltration; D018074:Vaccines, Conjugate / Q000737:chemistry* / Q000302:isolation & purification
C408982:CPG-oligonucleotide; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D009838:Oligodeoxyribonucleotides; D018074:Vaccines, Conjugate