title: Determination of muscimol and ibotenic acid in Amanita mushrooms by high-performance liquid chromatography and liquid chromatography-tandem mass spectrometry.
abstract: A reliable analytical method was developed for the quantification and identification of muscimol (MUS) and ibotenic acid (IBO), the toxic constituents of Amanita muscaria and Amanita pantherina. MUS and IBO were extracted from mushrooms by aqueous methanol and derivatized with dansyl chloride (DNS-Cl). After extraction with ethyl acetate and evaporation of the solvent, the residue was ethylated with 1.25 M hydrogen chloride in ethanol. The resulting derivatives were quantified by high-performance liquid chromatography with UV detection and identified by liquid chromatography electrospray ionization tandem mass spectrometry. Calibration curves were linear in the range of 25-2500 ppm for MUS and 40-2500 ppm for IBO, respectively. This method was successfully applied to identify and quantify MUS and IBO in Amanita mushrooms naturally grown and circulated in the drug market.
authors.affiliation: National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan. tujikawa@nrips.go.jp
authors.forename: Kenji
authors.initials: K
authors.lastname: Tsujikawa
authors.forename: Kenji
authors.initials: K
authors.lastname: Kuwayama
authors.forename: Hajime
authors.initials: H
authors.lastname: Miyaguchi
authors.forename: Tatsuyuki
authors.initials: T
authors.lastname: Kanamori
authors.forename: Yuko
authors.initials: Y
authors.lastname: Iwata
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Inoue
authors.forename: Takemi
authors.initials: T
authors.lastname: Yoshida
authors.forename: Tohru
authors.initials: T
authors.lastname: Kishi
mesh_terms: D000545:Amanita / Q000737:chemistry*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007051:Ibotenic Acid / Q000032:analysis*; D009118:Muscimol / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013056:Spectrophotometry, Ultraviolet; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D007051:Ibotenic Acid; D009118:Muscimol