title: N-3 PUFAs modulate global gene expression profile in cultured rat cardiomyocytes. Implications in cardiac hypertrophy and heart failure.
abstract: In cardiac cells the effects of n-3 PUFAs on the whole genome are still unknown despite their recognized cardioprotective effects and ability to modulate gene expression. We have evaluated the effects of n-3 PUFAs supplementation on the global gene expression profile in cultured neonatal rat cardiomyocytes, detecting many genes related to lipid transport and metabolism among the upregulated ones. Many of the downregulated genes appeared related to inflammation, cell growth, extracellular and cardiac matrix remodelling, calcium movements and ROS generation. Our data allow to speculate that the cardioprotective effect of n-3 PUFAs is related to a direct modulation of genes in cardiac cells.
authors.affiliation: Nutrition Research Center, Department of Biochemistry "G. Moruzzi", University of Bologna, Via Irnerio, 48 - 40126 Bologna, Italy. alessandra.bordoni@unibo.it
authors.forename: Alessandra
authors.initials: A
authors.lastname: Bordoni
authors.forename: Annalisa
authors.initials: A
authors.lastname: Astolfi
authors.forename: Luca
authors.initials: L
authors.lastname: Morandi
authors.forename: Andrea
authors.initials: A
authors.lastname: Pession
authors.forename: Francesca
authors.initials: F
authors.lastname: Danesi
authors.forename: Mattia
authors.initials: M
authors.lastname: Di Nunzio
authors.forename: Monica
authors.initials: M
authors.lastname: Franzoni
authors.forename: Pierluigi
authors.initials: P
authors.lastname: Biagi
authors.forename: Annalisa
authors.initials: A
authors.lastname: Pession
mesh_terms: D000818:Animals; D001483:Base Sequence; D006332:Cardiomegaly / Q000235:genetics / Q000517:prevention & control; D002316:Cardiotonic Agents / Q000494:pharmacology; D002478:Cells, Cultured; D017931:DNA Primers / Q000235:genetics; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D006333:Heart Failure / Q000235:genetics / Q000517:prevention & control; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism*; D051381:Rats
chemical_list: D002316:Cardiotonic Agents; D017931:DNA Primers; D015525:Fatty Acids, Omega-3