title: Effect of haemin on growth, protein content and the antioxidant defence system in Trypanosoma cruzi.
abstract: A nutritional characteristic of trypanosomatid protozoa is that in vitro they need a haem-compound as a growth factor, which is supplied as haemoglobin, haematin or haemin. Because haemin and related porphyrins are an important source of oxidative stress in biological systems, the effect of haemin on growth, protein content and the antioxidant defence system in Trypanosoma cruzi was evaluated. We have observed that, in epimastigotes grown under different haemin concentrations in the culture medium (0-30 mg/l), 5 mg/l was the haemin concentration yielding optimum growth. Above 15 mg/l there was a clear decrease in growth rate, producing the epimastigote to amastigote transformation. Such morphological change was observed together with a marked injury of the enzymatic machinery of the parasite, leading to diminished protein synthesis as well as lower activity of the antioxidant enzymes (superoxide dismutase, ascorbate peroxidase and trypanothione reductase), reduced total thiol content and a marked increase in the HaemOx-1 activity and expression. The current work demonstrates that there is a correlation between higher haemin concentrations in the culture medium and oxidative damage in the cells. Under these conditions induction of HaemOx-1 would indicate the important role of this enzyme as an antioxidant defence response in Trypanosoma cruzi.
authors.affiliation: Centro de Investigaciones sobre Porfirinas y Porfirias, CONICET-UBA, Argentina.
authors.forename: A
authors.initials: A
authors.lastname: Ciccarelli
authors.forename: L
authors.initials: L
authors.lastname: Araujo
authors.forename: A
authors.initials: A
authors.lastname: Batlle
authors.forename: E
authors.initials: E
authors.lastname: Lombardo
mesh_terms: D000818:Animals; D060387:Ascorbate Peroxidases; D003470:Culture Media; D015870:Gene Expression / Q000187:drug effects; D051547:Heme Oxygenase-1 / Q000032:analysis / Q000096:biosynthesis; D006427:Hemin / Q000494:pharmacology*; D009247:NADH, NADPH Oxidoreductases / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D010544:Peroxidases / Q000187:drug effects / Q000378:metabolism; D011506:Proteins / Q000032:analysis / Q000187:drug effects; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013482:Superoxide Dismutase / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D014349:Trypanosoma cruzi / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology*
chemical_list: D003470:Culture Media; D011506:Proteins; D013438:Sulfhydryl Compounds; D006427:Hemin; D010544:Peroxidases; D060387:Ascorbate Peroxidases; D051547:Heme Oxygenase-1; D013482:Superoxide Dismutase; D009247:NADH, NADPH Oxidoreductases; C049608:trypanothione reductase