Reliable universal RT-PCR assays for studying influenza polymerase subunit gene sequences from all 16 haemagglutinin subtypes.
Realizable one-step RT-PCR assays specific for influenza PB2, PB1 and PA segments are described in this report. The designs of the consensus primers were based on more than five thousands polymerase genes derived from avian or mammalian viral strains. All the viral RNA tested in this study could be consistently amplified by the assays. The reaction products were specific and could be used for direct DNA sequencing. These assays might be useful tools to study the sequences of these genes.
Li
Olive T W
OT
State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong SAR, China.
Barr
Ian
I
Leung
Connie Y H
CY
Chen
Honglin
H
Guan
Yi
Y
Peiris
J S Malik
JS
Poon
Leo L M
LL
D000818:Animals; D001483:Base Sequence; D016384:Consensus Sequence; D017931:DNA Primers; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000145:classification* / Q000235:genetics; D006801:Humans; D009980:Influenza A virus / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*
D017931:DNA Primers; D019267:Hemagglutinin Glycoproteins, Influenza Virus; C061290:PA protein, influenza viruses; C057027:PB2 protein, Influenzavirus A; D014764:Viral Proteins; C077346:influenza virus polymerase basic protein 1; D012324:RNA-Dependent RNA Polymerase