Paenibacillus strain MP-1: a new source of mutanase.
A mutan-degrading bacterium, closely related to Paenibacillus curdlanolyticus, was isolated from soil. It produced 0.4 U mutanase ml(-1 )in 2 days in shake-flask cultures when bacterial mutan was the sole carbon source. Mutanase activity was optimal at pH 6.2 and 45 degrees C over 1 h and was stable between pH 5.8 and 12 at 4 degrees C for 24 h and up to 40 degrees C for 1 h. Mutan produced by Streptococcus mutans was rapidly hydrolyzed by this enzyme. The hydrolysis of mutan (1 g l(-1)) resulted in 17% saccharification over 2 h and, at the same time, glucan was entirely solubilized.
Pleszczyńska
M
M
Department of Industrial Microbiology, Maria Curie-Skłodowska University, Akademicka 19, 20-033, Lublin, Poland. mplesz@poczta.onet.pl
Marek-Kozaczuk
M
M
Wiater
A
A
Szczodrak
J
J
D001407:Bacillus / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification; D002244:Carbon / Q000494:pharmacology; D005936:Glucans / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000096:biosynthesis / Q000378:metabolism*; D006868:Hydrolysis / Q000187:drug effects; D013997:Time Factors
D005936:Glucans; D002244:Carbon; D006026:Glycoside Hydrolases; C021780:exo-1,3-alpha-glucanase