Hemocytes ultrastructure of Aedes aegypti (Diptera: Culicidae).
Mosquitoes have an efficient defence system against infection. Insect blood cells (hemocytes) play an essential role in defense against parasites and other pathogenic organisms that infect insects. We have identified by light and transmission electron microscopy six hemocytes cell types from the hemolymph of Aedes aegypti. They were: prohemocytes (20%), adipohemocytes (29%), granulocytes (16%), plasmatocytes (27%), oenocytoids (7%) and thrombocytoids (0.9%). The prohemocytes were the smallest hemocytes found in the hemolymph. Its cytoplasm occupies only a narrow area around the nucleus. The adipohemocytes were the most abundant cell type presented. These hemocytes exhibited a large lipid like vesicle and mitochondria. In electron micrographs, the granulocytes showed cytoplasm containing dilated rough endoplasmic reticulum (RER) and a round or elongated mitochondria. Electron-dense granules with a proteinaceous material were also present. The plasmatocytes were polymorphic and exhibited plasma membrane with irregular processes, philopodia and pseudopodia. Ultrastructural investigation revealed that the reticular cytoplasm showed a well-developed RER, a Golgi and vacuoles. Oenocytoids showed homogeneous cytoplasm with many mitochondria and ribosomes are scattered throughout the cytoplasm, abundant RER and a small smooth endoplasmic reticulum (SER) present at the cell poles. Thrombocytoids were very fragile and few in number. Similar characteristics were found in oenocytoids, possessing a homogeneous cytoplasm with poorly developed organelles, few mitochondria and granules.
Araújo
H C R
HC
Departamento de Biologia Celular e Ultra-estrutura, Universidade Federal de Pernambuco, Brazil. helena@cpqam.fiocruz.br
Cavalcanti
M G S
MG
Santos
S S
SS
Alves
L C
LC
Brayner
F A
FA
D000330:Aedes / Q000648:ultrastructure*; D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D006434:Hemocytes / Q000145:classification / Q000648:ultrastructure*; D008854:Microscopy, Electron