title: Effect of dietary intervention with different pre- and probiotics on intestinal bacterial enzyme activities.
abstract: OBJECTIVE
  To investigate the influence of different pre- and probiotics on faecal beta-glucuronidase and beta-glucosidase activity, as one of the claimed beneficial effects of pre- and probiotics is the hypothesis that these substrates are able to reduce the production of toxic and carcinogenic metabolites by suppressing specific enzyme activities in the colon.
  
  
  METHODS
  Department of Gastrointestinal Research, University Hospital Gasthuisberg, KU Leuven, Belgium.
  
  
  METHODS
  The effect was evaluated in a randomized, crossover study in 53 healthy volunteers who were randomly assigned to one of five treatment groups.
  
  
  METHODS
  At the start and after a 4-week treatment period, the healthy volunteers collected faeces during 72 h. Lactulose and oligofructose-enriched inulin (OF-IN) were chosen as prebiotics, whereas Lactobacillus casei Shirota, Bifidobacterium breve and Saccharomyces boulardii were selected as probiotics. Two synbiotic combinations were evaluated as well. The enzyme activity was assessed spectrophotometricly.
  
  
  RESULTS
  Lactulose and OF-IN significantly decreased beta-glucuronidase activity, whereas a tendency to a decreased beta-glucuronidase activity was observed after L. casei Shirota and B. breve intake. To the contrary, B. breve increased beta-glucosidase levels. Supplementation with the synbiotic did not appear to be more beneficial than either compound alone. No influence of S. boulardii was noted.
  
  
  CONCLUSIONS
  Administration of lactulose, OF-IN, L. casei Shirota or B. breve resulted in a decrease of the beta-glucuronidase activity, which is considered beneficial for the host.
authors.affiliation: Department of Gastrointestinal Research, University Hospital Gasthuisberg, Leuven, Belgium.
authors.forename: V
authors.initials: V
authors.lastname: De Preter
authors.forename: H
authors.initials: H
authors.lastname: Raemen
authors.forename: L
authors.initials: L
authors.lastname: Cloetens
authors.forename: E
authors.initials: E
authors.lastname: Houben
authors.forename: P
authors.initials: P
authors.lastname: Rutgeerts
authors.forename: K
authors.initials: K
authors.lastname: Verbeke
mesh_terms: D000328:Adult; D001644:Bifidobacterium / Q000502:physiology; D003106:Colon / Q000201:enzymology; D018592:Cross-Over Studies; D004305:Dose-Response Relationship, Drug; D005243:Feces / Q000201:enzymology*; D005260:Female; D005966:Glucuronidase / Q000378:metabolism*; D006801:Humans; D007444:Inulin / Q000494:pharmacology*; D007780:Lacticaseibacillus casei / Q000502:physiology; D007792:Lactulose / Q000494:pharmacology; D008297:Male; D009844:Oligosaccharides / Q000494:pharmacology; D019936:Probiotics; D012440:Saccharomyces / Q000502:physiology; D013053:Spectrophotometry / Q000379:methods; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D009844:Oligosaccharides; C120489:oligofructose; D007792:Lactulose; D007444:Inulin; D001617:beta-Glucosidase; D005966:Glucuronidase