Protein profiling of microdissected pancreas carcinoma and identification of HSP27 as a potential serum marker.
BACKGROUND
Patients with pancreatic adenocarcinomas have a poor prognosis because of late clinical manifestation and the tumor's aggressive nature. We used proteomic techniques to search for markers of pancreatic carcinoma.


METHODS
We performed protein profiling of microdissected cryostat sections of 9 pancreatic adenocarcinomas and 10 healthy pancreatic tissue samples using ProteinChip technology (surface-enhanced laser desorption/ionization). We identified proteins by use of 2-dimensional gel electrophoresis, peptide fingerprint mapping, and immunodepletion and used immunohistochemistry for in situ localization of the proteins found. We used ELISA to quantify these proteins in preoperative serum samples from 35 patients with pancreatic cancer and 37 healthy individuals.


RESULTS
From among the differentially expressed signals that were detected by ProteinChip technology, we identified 2 proteins, DJ-1 and heat shock protein 27 (HSP27). We then detected HSP27 in sera of patients by use of ELISA, indicating a sensitivity of 100% and a specificity of 84% for the recognition of pancreatic cancer.


CONCLUSIONS
The detection of DJ-1 and HSP27 in pure defined tissue and the retrieval of HSP27 in serum by antibody-based methods identifies a potential marker for pancreatic cancer.
Melle
Christian
C
Core Unit Chip Application, Institute of Human Genetics and Anthropology, Medical Faculty at Friedrich Schiller University Jena, Jena, Germany.
Ernst
Günther
G
Escher
Niko
N
Hartmann
Daniel
D
Schimmel
Bettina
B
Bleul
Annett
A
Thieme
Heike
H
Kaufmann
Roland
R
Felix
Klaus
K
Friess
Helmut M
HM
Settmacher
Utz
U
Hommann
Merten
M
Richter
Konrad K
KK
Daffner
Wolfgang
W
Täubig
Horst
H
Manger
Thomas
T
Claussen
Uwe
U
von Eggeling
Ferdinand
F
D000230:Adenocarcinoma / Q000175:diagnosis* / Q000473:pathology; D014408:Biomarkers, Tumor / Q000032:analysis*; D015153:Blotting, Western; D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D042282:Microdissection; D018832:Molecular Chaperones; D009363:Neoplasm Proteins / Q000032:analysis*; D010190:Pancreatic Neoplasms / Q000175:diagnosis* / Q000473:pathology; D040081:Protein Array Analysis; D020543:Proteome / Q000032:analysis*
D014408:Biomarkers, Tumor; D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D009363:Neoplasm Proteins; D020543:Proteome