Magnesium ion selective two-photon fluorescent probe based on a benzo[h]chromene derivative for in vivo imaging.
A novel, two-photon probe for the detection of free Mg2+ ions in living cells and live tissues has been developed. The probe can be excited by 880 nm laser photons, emits strong two-photon excited fluorescence in response to Mg2+ ions, can be easily loaded into the cell and tissue, shows high photostability, and can measure the Mg2+ ion concentration without interference by Ca2+ ions in living cells. The intracellular dissociation constant (Kdi) for Mg2+ determined by the two-photon process is 2.5 mM, which is suitable for dynamic Mg2+ concentration measurement. In addition, the probe is capable of imaging endogenous stores of free Mg2+ at a few hundred micrometers depth in live tissues using two-photon microscopy (TPM).
Kim
Hwan Myung
HM
Molecular Opto-Electronics Laboratory, Department of Chemistry and Center for Electro- and Photo-Responsive Molecules, Graduate School of Life Sciences and Biotechnology, Korea University, 1-Anamdong, Seoul 136-701, Korea.
Yang
Pil Rye
PR
Seo
Mun Sik
MS
Yi
Jae-Sung
JS
Hong
Jin Hee
JH
Jeon
Seung-Joon
SJ
Ko
Young-Gyu
YG
Lee
Kyoung J
KJ
Cho
Bong Rae
BR
D000818:Animals; D001578:Benzopyrans; D002477:Cells / Q000737:chemistry; D005456:Fluorescent Dyes; D006801:Humans; D008274:Magnesium / Q000032:analysis* / Q000493:pharmacokinetics; D017785:Photons
D001578:Benzopyrans; D005456:Fluorescent Dyes; D008274:Magnesium