title: TRPM2 immunoreactivity is increased in fibroblasts, but not nerves, of symptomatic human dental pulp.
abstract: Transient receptor potential (TRP) channels function in diverse processes such as acting as second messenger systems, regulating of ionic concentrations, and aiding in thermoception. TRPM2 channels, members of the melastatin subfamily, mediate calcium influx in response to oxidative stress but during pathological states facilitate hyperexcitability and cellular necrosis via calcium excitotoxicity. We hypothesized that TRPM2 channel expression is upregulated in pulpal tissue of symptomatic teeth with signs of irreversible pulpitis. TRPM2 channel expression was significantly increased in pulp from clinically diagnosed symptomatic teeth compared with pulp from asymptomatic teeth. Additionally, increased TRPM2 expression in symptomatic pulp was the result of increased immunoreactivity in fibroblasts, whereas neural expression of TRPM2 was absent. We provide a possible mechanism explaining the association between TRPM2 channel expression with pain and necrosis. We suggest that TRPM2 channel antagonists could be administered in attempts to inhibit the progression of or even reverse pulpal degradation.
authors.affiliation: Southern Illinois University, School of Dental Medicine, Alton, Illinois 62002, USA. krowlan@siue.edu
authors.forename: Kevin C
authors.initials: KC
authors.lastname: Rowland
authors.forename: Courtney B
authors.initials: CB
authors.lastname: Kanive
authors.forename: Jason E
authors.initials: JE
authors.lastname: Wells
authors.forename: John F
authors.initials: JF
authors.lastname: Hatton
mesh_terms: D001369:Axons / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D003782:Dental Pulp / Q000166:cytology / Q000294:innervation / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D011671:Pulpitis / Q000378:metabolism*; D050053:TRPM Cation Channels / Q000032:analysis / Q000096:biosynthesis*; D014098:Toothache / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D050053:TRPM Cation Channels; C121934:TRPM2 protein, human; D002118:Calcium