title: Magnesium ion selective two-photon fluorescent probe based on a benzo[h]chromene derivative for in vivo imaging.
abstract: A novel, two-photon probe for the detection of free Mg2+ ions in living cells and live tissues has been developed. The probe can be excited by 880 nm laser photons, emits strong two-photon excited fluorescence in response to Mg2+ ions, can be easily loaded into the cell and tissue, shows high photostability, and can measure the Mg2+ ion concentration without interference by Ca2+ ions in living cells. The intracellular dissociation constant (Kdi) for Mg2+ determined by the two-photon process is 2.5 mM, which is suitable for dynamic Mg2+ concentration measurement. In addition, the probe is capable of imaging endogenous stores of free Mg2+ at a few hundred micrometers depth in live tissues using two-photon microscopy (TPM).
authors.affiliation: Molecular Opto-Electronics Laboratory, Department of Chemistry and Center for Electro- and Photo-Responsive Molecules, Graduate School of Life Sciences and Biotechnology, Korea University, 1-Anamdong, Seoul 136-701, Korea.
authors.forename: Hwan Myung
authors.initials: HM
authors.lastname: Kim
authors.forename: Pil Rye
authors.initials: PR
authors.lastname: Yang
authors.forename: Mun Sik
authors.initials: MS
authors.lastname: Seo
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Yi
authors.forename: Jin Hee
authors.initials: JH
authors.lastname: Hong
authors.forename: Seung-Joon
authors.initials: SJ
authors.lastname: Jeon
authors.forename: Young-Gyu
authors.initials: YG
authors.lastname: Ko
authors.forename: Kyoung J
authors.initials: KJ
authors.lastname: Lee
authors.forename: Bong Rae
authors.initials: BR
authors.lastname: Cho
mesh_terms: D000818:Animals; D001578:Benzopyrans; D002477:Cells / Q000737:chemistry; D005456:Fluorescent Dyes; D006801:Humans; D008274:Magnesium / Q000032:analysis* / Q000493:pharmacokinetics; D017785:Photons
chemical_list: D001578:Benzopyrans; D005456:Fluorescent Dyes; D008274:Magnesium