Expression of matrix metalloproteinases of human lens epithelial cells in the cultured lens capsule bag.
AIM OF PURPOSE: To observe the different expression of matrix metalloproteinases (MMPs) between pre- and postoperation of sham cataract surgery in vitrohuman lens capsule bag model from the same donor eye in order to investigate a possible role of MMPs in posterior capsule opacification (PCO).


METHODS
Sham cataract surgeries were performed in six human donor eyes. Immunohistochemical staining was used to detect the expression of MMP-2 and -9 of human lens epithelial cells (LECs) on the anterior capsulorhexis. LEC migration on posterior capsule of human lens from the same donor eye was observed in a modified capsule bag model without pin. Total MMP-2 and -9 protein production were determined by enzyme-linked immunosorbent assay at days 2, 10, 20, and 30 postoperation, respectively.


RESULTS
MMP-2 and -9 could not be detected immunohistochemically on the anterior capsulorhexis preoperation of cataract. Lens epithelia cells at the equator began to migrate by day 4. A confluent monolayer of lens epithelia cells was present on the posterior capsule at day 20. Total MMP-2 and -9 protein production increased with time with maximum levels reached on day 30.


CONCLUSIONS
MMP-2 and -9 were showed to be upregulated following sham cataract surgery. MMP expression could play an important role in PCO.
Li
J H
JH
Beijing Tongren Eye Center, Beijing Tongren Hospital, Affiliated Hospital of Capital Medical University, Beijing, China.
Wang
N L
NL
Wang
J J
JJ
D002387:Cataract Extraction; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000201:enzymology*; D005260:Female; D006801:Humans; D007908:Lens, Crystalline / Q000201:enzymology*; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D008875:Middle Aged
C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9