Organization of flaviviral replicase proteins in virus-induced membranes: a role for NS1' in Japanese encephalitis virus RNA synthesis.
The organization of flaviviral replicase proteins within the membrane-bound replication complexes of West Nile (WNV), dengue (DENV) and Japanese encephalitis viruses (JEV) was probed by investigating the combined effect of detergents and trypsin on both viral replicase activity and profile of metabolically labelled viral proteins. While trypsin treatment of virus-induced membrane fractions degraded the vast majority of replicase proteins, viral RNA-dependent RNA polymerase (RdRp) activity remained completely unaffected. Solubilization of the membranes with deoxycholate (DOC) however rendered the replicase accessible to trypsin. Triton X-100 (TX100) treatment reduced RdRp activity by half in WNV but totally destroyed RdRp activity in JEV. TX100 also dissociated NS1' in addition to NS1 from NS5 and NS3 inJEV. Antibodies to NS3 coprecipitated NS1' along with NS5 only from DOC-solubilized but not from TX100-treated extracts, the former of which alone retained RdRp activity. Exogenous addition of recombinant NS1' to TX100 treated JEV-induced membranes restored the defect in the release step of RNA synthesis. Our results suggest for the first time a direct role for JEV NS1' in viral RNA synthesis in vitro.
Satchidanandam
Vijaya
V
Department of Microbiology and Cell Biology, Sir C.V. Raman Avenue, Indian Institute of Science, Bangalore 560012, India.
Uchil
Pradeep Devappa
PD
Kumar
Priti
P
D015153:Blotting, Western; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D004664:Encephalitis Virus, Japanese / Q000235:genetics*; D020365:RNA Helicases / Q000737:chemistry / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism*; D015531:Radioimmunoprecipitation Assay; D012697:Serine Endopeptidases / Q000737:chemistry / Q000378:metabolism; D014357:Trypsin / Q000494:pharmacology; D017361:Viral Nonstructural Proteins / Q000737:chemistry* / Q000378:metabolism; D014779:Virus Replication; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
D020365:RNA Helicases; D012367:RNA, Viral; D012697:Serine Endopeptidases; D014357:Trypsin; D017361:Viral Nonstructural Proteins; C100315:NS1 protein, Flavivirus; C060028:NS3 protein, flavivirus; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases; C096041:NS5 protein, flavivirus