title: Pepsin assay: a marker for reflux in pediatric glue ear.
abstract: OBJECTIVE
  To evaluate if analysis of pepsin/pepsinogen in middle ear effusions can be considered a diagnostic marker for laryngopharyngeal reflux (LPR) in children with otitis media with effusion (OME).
  
  
  METHODS
  Ambulatory 24-hour dual-probe pH monitoring was carried out on 31 children with OME. Middle ear effusions were collected from 17 children during myringotomy. Total pepsin/pepsinogen concentrations in effusions were measured by ELISA using antipepsin antibody.
  
  
  RESULTS
  Dual-probe pH monitoring showed that 22/31 (71%) of the studied children had significant LPR. The concentrations of pepsin/pepsinogen in middle ear effusions, ranged from 0.085 to 5.02 microg/ml, were found to be up to 4.5 to 231.44 times higher than the serum levels. There was a significant positive correlation between the level of pepsin/pepsinogen assayed in the effusions of the 17 children and the number of pharyngeal reflux episodes measured by pH monitoring.
  
  
  CONCLUSIONS
  Control of LPR may be an essential component in the successful management of OME in pediatric patients. Pepsin/pepsinogen analysis in effusions of children, using ELISA, can be considered a reliable marker for assessment of reflux in children with OME.
authors.affiliation: Department of Otolaryngology, Mansoura University, Mansoura, Egypt.
authors.forename: Ahmed M
authors.initials: AM
authors.lastname: Abd El-Fattah
authors.forename: Gamal A
authors.initials: GA
authors.lastname: Abdul Maksoud
authors.forename: Ahmed S
authors.initials: AS
authors.lastname: Ramadan
authors.forename: Ahmed F
authors.initials: AF
authors.lastname: Abdalla
authors.forename: Mohamed M
authors.initials: MM
authors.lastname: Abdel Aziz
mesh_terms: D000233:Adenoidectomy; D015415:Biomarkers / Q000032:analysis / Q000097:blood; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D050782:Esophageal pH Monitoring; D005764:Gastroesophageal Reflux / Q000175:diagnosis* / Q000503:physiopathology; D006801:Humans; D007013:Hypopharynx / Q000503:physiopathology*; D007223:Infant; D008876:Middle Ear Ventilation; D018670:Monitoring, Ambulatory / Q000379:methods; D010034:Otitis Media with Effusion / Q000175:diagnosis* / Q000503:physiopathology; D010434:Pepsin A / Q000032:analysis* / Q000097:blood; D020116:Pepsinogen A / Q000032:analysis* / Q000097:blood; D011446:Prospective Studies; D014068:Tonsillectomy
chemical_list: D015415:Biomarkers; D020116:Pepsinogen A; D010434:Pepsin A