title: Absorbents as packing materials in on-line coupling of reversed phase liquid chromatography and gas chromatography via a programmed temperature vaporizer.
abstract: A method based on the use of absorbents as packing materials in the interface of the direct coupling between reversed phase liquid chromatography and gas chromatography (RPLC-GC) is proposed. To that end, a comparative study on different adsorbents and absorbents was carried out. Specifically, Tenax TA and Gaschrom were used as adsorbents while polydimethylsiloxane and poly(50% phenyl:50% methylsiloxane) were the absorbents tested. Some experimental variables involved in the solvent elimination were separately optimised for adsorbent and absorbent materials. Relative standard deviations (RSD) lower than 10% were achieved in all cases but the use of absorbents showed interesting advantages with respect to adsorbents, namely a simpler performance of the experimental work, which facilitates the sample preparation step and the subsequent gas chromatographic analysis to be performed.
authors.affiliation: Instituto de Fermentaciones Industriales, Consejo Superior de Investigaciones Científicas (CSIC), c/ Juan de la Cierva 3, 28006 Madrid, Spain.
authors.forename: Gema
authors.initials: G
authors.lastname: Flores
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Ruiz Del Castillo
authors.forename: Marta
authors.initials: M
authors.lastname: Herraiz
mesh_terms: D000042:Absorption; D002849:Chromatography, Gas / Q000295:instrumentation / Q000379:methods*; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D004129:Dimethylpolysiloxanes; D009862:Online Systems; D011108:Polymers / Q000737:chemistry; D012828:Silicones; D012833:Siloxanes
chemical_list: D004129:Dimethylpolysiloxanes; D011108:Polymers; D012828:Silicones; D012833:Siloxanes; C010514:tenax; C014763:polymethylphenylsiloxane; C013830:baysilon