In situ studies of protein adsorptions on poly(pyrrole-co-pyrrole propylic acid) film by electrochemical surface plasmon resonance.
Poly(pyrrole-co-pyrrole propylic acid) (PPy/PPa) composite films were prepared for the first time by electrochemical copolymerization in mixed pyrrole propylic acid (Pa) and pyrrole solutions. The electrochemical growth process was investigated by in situ electrochemical surface plasmon resonance (ESPR). Atomic force microscopy and Fourier transform infrared spectroscopy were applied to characterize the prepared films. Using bovine serum albumin as a model protein, the adsorption kinetics of the protein on PPy/PPa films were studied in situ by SPR. The composition of Pa, the isoelectric point of proteins, the pH of buffers, and surfactant treatment showed dramatic effects on the protein adsorption on the PPy/PPa film. Experimental results indicated that the electrostatic interaction between the PPy/PPa film and proteins plays a critical role in protein adsorption and provided a novel strategy to efficiently immobilize proteins and to reduce nonspecific bindings of proteins in an immunobiosensor.
Hu
Weihua
W
School of Chemical & Biomedical Engineering, Center for Advanced Bionanosystems, Nanyang Technological University, 70 Nanyang Drive, Singapore 637457.
Li
Chang Ming
CM
Cui
Xiaoqiang
X
Dong
Hua
H
Zhou
Qin
Q
D000327:Adsorption; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation; D002021:Buffers; D002417:Cattle; D004563:Electrochemistry / Q000379:methods; D006863:Hydrogen-Ion Concentration; D018625:Microscopy, Atomic Force / Q000379:methods; D011422:Propionates / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D020349:Surface Plasmon Resonance / Q000379:methods*; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry
D002021:Buffers; D011422:Propionates; D011506:Proteins; D011758:Pyrroles; D013501:Surface-Active Agents; C518858:polypyrrolepropylic acid; D012710:Serum Albumin, Bovine