title: Proteomic analyses of songbird (Zebra finch; Taeniopygia guttata) retina.
abstract: Proteomic analyses of male songbird (Zebra finch; Taeniopygia guttata; ZF) retina were performed resulting in identification of 129 proteins. Comparison of T. guttata retinal proteome with that of chicken found proteins detected in both retinas. Immunohistochemical analyses of T. guttata retinal sections and Western analyses of total retinal protein extract were performed confirming presence of select bona fide retinal proteins. Results demonstrate the utility of one-dimensional gel fractionation for mass spectrometry and will be useful for future proteomic comparison of songbird retina and brain tissues in different behavioral and pharmacological studies.
authors.affiliation: Bascom Palmer Eye Institute, University of Miami, Miami, Florida, 33136, USA.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Sloley
authors.forename: Shannon
authors.initials: S
authors.lastname: Smith
authors.forename: Mabel
authors.initials: M
authors.lastname: Algeciras
authors.forename: Valerie
authors.initials: V
authors.lastname: Cavett
authors.forename: Jennifer A Caldwell
authors.initials: JA
authors.lastname: Busby
authors.forename: Sarah
authors.initials: S
authors.lastname: London
authors.forename: David F
authors.initials: DF
authors.lastname: Clayton
authors.forename: Sanjoy K
authors.initials: SK
authors.lastname: Bhattacharya
mesh_terms: D000818:Animals; D001717:Birds / Q000235:genetics*; D015153:Blotting, Western; D002645:Chickens; D008297:Male; D013058:Mass Spectrometry; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D012160:Retina / Q000737:chemistry*
chemical_list: D011506:Proteins