title: Transgenic American elm shows reduced Dutch elm disease symptoms and normal mycorrhizal colonization.
abstract: The American elm (Ulmus americana L.) was once one of the most common urban trees in eastern North America until Dutch-elm disease (DED), caused by the fungus Ophiostoma novo-ulmi, eliminated most of the mature trees. To enhance DED resistance, Agrobacterium was used to transform American elm with a transgene encoding the synthetic antimicrobial peptide ESF39A, driven by a vascular promoter from American chestnut. Four unique, single-copy transgenic lines were produced and regenerated into whole plants. These lines showed less wilting and significantly less sapwood staining than non-transformed controls after O. novo-ulmi inoculation. Preliminary observations indicated that mycorrhizal colonization was not significantly different between transgenic and wild-type trees. Although the trees tested were too young to ensure stable resistance was achieved, these results indicate that transgenes encoding antimicrobial peptides reduce DED symptoms and therefore hold promise for enhancing pathogen resistance in American elm.
authors.affiliation: Faculty of Environmental and Forest Biology, SUNY College of Environmental Science and Forestry, Syracuse, NY, USA.
authors.forename: Andrew E
authors.initials: AE
authors.lastname: Newhouse
authors.forename: Franziska
authors.initials: F
authors.lastname: Schrodt
authors.forename: Haiying
authors.initials: H
authors.lastname: Liang
authors.forename: Charles A
authors.initials: CA
authors.lastname: Maynard
authors.forename: William A
authors.initials: WA
authors.lastname: Powell
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D008826:Microbial Sensitivity Tests; D038821:Mycorrhizae / Q000502:physiology*; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology; D030821:Plants, Genetically Modified; D019076:Transgenes / Q000235:genetics; D034403:Ulmus / Q000235:genetics* / Q000382:microbiology*
chemical_list: D023181:Antimicrobial Cationic Peptides; C522208:antimicrobial peptide ESF39A