title: Development of dextran-derivative arrays to identify physicochemical properties involved in biofouling from serum.
abstract: To control protein adsorption on surfaces, low-fouling polymer coatings such as poly(ethylene oxide) (PEG or PEO) and polysaccharides are used. Their ability to resist protein adsorption is related to the layer structure, hence the immobilization mode. A polymer array technology was developed to study the structural diversity of carboxymethyl dextran (CMD) layers, whose immobilization conditions were varied. CMD arrays were analyzed by X-ray photoelectron spectroscopy (XPS) and by atomic force microscopy (AFM) colloidal probe force measurements. Serum protein adsorption was studied directly on the CMD arrays using surface plasmon resonance (SPR) microscopy. Physicochemical characterization revealed that pinning density regulates surface coverage and the amount of adsorbed molecules, and that salt concentration influences the surface structure of the charged polymer, forming extended or short layers. Protein adsorption experiments from serum showed that repulsive CMD layers are dense, with extended flexible chains. The present study underlines the usefulness of polymer arrays to study structural diversity of thin graft layers and to relate their physicochemical properties to their resistance to nonspecific protein adsorption.
authors.affiliation: Laboratoire de Bioingénierie et de Biophysique de l'Université de Sherbrooke, Department of Chemical Engineering, Université de Sherbrooke, 2500 blvd de l'Université, Sherbrooke, Québec, Canada J1K 2R1.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Monchaux
authors.forename: Patrick
authors.initials: P
authors.lastname: Vermette
mesh_terms: D000327:Adsorption; D017609:Barium Compounds / Q000737:chemistry; D002627:Chemistry, Physical / Q000379:methods*; D003911:Dextrans / Q000737:chemistry*; D006801:Humans; D018625:Microscopy, Atomic Force; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D044967:Serum / Q000737:chemistry*; D017640:Silicates / Q000737:chemistry; D013052:Spectrometry, X-Ray Emission; D020349:Surface Plasmon Resonance; D013499:Surface Properties
chemical_list: D017609:Barium Compounds; D003911:Dextrans; D011108:Polymers; D011134:Polysaccharides; D011506:Proteins; D017640:Silicates; D011092:Polyethylene Glycols