Genetic variation within the glycoprotein B and H genes of human cytomegalovirus in solid organ transplant recipients.
This study was performed to investigate human cytomegalovirus (HCMV) infection and genetic variations within glycoprotein B (gB) and H (gH) genes in Chinese transplant recipients. A total of 245 ethylene-diamine tetraacetic acid (EDTA)-treated blood samples were obtained from 79 transplant recipients in southeast China. Based on the sequences of highly variable regions of the gB endoprotease cleavage site (gBclv), N-terminus of gp116 (gBn), and the gH N-terminus (gH), nested polymerase chain reaction assays for the detection of HCMV were established. Nucleotide sequencing was employed to differentiate gB and gH genotypes. Twenty-six of 79 (32.9%) transplant recipients were proved HCMV positive. The distribution of genotypes was gBclv1, 12/25; gBclv2, 3/25; gBclv3, 4/25; gBn1, 6/23; gBn2, 2/23; gBn3, 11/23; and no gBclv/n 4-related genotypes were presented. The distribution of gH genotypes was gH1, 11/26; gH2, 9/26; and co-infected with both gH1/2 in 6/26. These data show that genetic variability within the gB genes occurs frequently. Mixtures of gB and gH genotypes infection were common in Chinese solid organ transplant recipients.
Zhou
L
L
Key Lab of Combined Multi-Organ Transplantation, Ministry of Public Health, The First Alliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Fan
J
J
Zheng
S S
SS
Ma
W H
WH
D000595:Amino Acid Sequence; D020134:Catalytic Domain / Q000235:genetics; D002681:China / Q000453:epidemiology; D003587:Cytomegalovirus / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000453:epidemiology* / Q000209:etiology; D005814:Genes, Viral; D014644:Genetic Variation; D006801:Humans; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D016377:Organ Transplantation; D016415:Sequence Alignment; D014759:Viral Envelope Proteins / Q000235:genetics*
D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus; C071440:glycoprotein H, Cytomegalovirus