Extraction of plant RNA.
Optimal sampling procedures for sampling plant tissue for RNA extractions are outlined in this chapter. To extract RNA, kits supplied from biotechnology companies are appropriate, but some procedures will not work with particular plant tissues. Two alternative methods are supplied for troublesome material. Basic methods to check RNA quantity and, more particularly, RNA quality before use are supplied.
MacRae
Elspeth
E
Biomaterials Research, Scion, Rotorua, New Zealand.
D017360:Arabidopsis / Q000235:genetics; D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D006146:Guanidines; D017879:Isothiocyanates; D008967:Molecular Biology / Q000379:methods; D010636:Phenols; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D018749:RNA, Plant / Q000235:genetics / Q000302:isolation & purification*
D002593:Cetrimonium Compounds; D006146:Guanidines; D017879:Isothiocyanates; D010636:Phenols; D012333:RNA, Messenger; D018749:RNA, Plant; C054435:guanidine isothiocyanate; C411644:trizol; D000077286:Cetrimonium