Construction of a pair of practical Nocardia-Escherichia coli shuttle vectors.
We constructed a pair of Nocardia-Escherichia coli shuttle vectors, pNV18 and pNV19, by combining the mycobacterial plasmid pAL5000 with the E. coli vector pK18 or pK19. These vectors have a number of useful features, including small size (4.4 kb), a multiple cloning site, and blue/white selection. To our knowledge, pNV18 and pNV19 are the first cloning vectors for practical use in Nocardia spp.
Chiba
Kazuhiro
K
Department of Bioactive Molecules, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
Hoshino
Yasutaka
Y
Ishino
Keiko
K
Kogure
Takahisa
T
Mikami
Yuzuru
Y
Uehara
Yoshimasa
Y
Ishikawa
Jun
J
D003001:Cloning, Molecular; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D007763:Lac Operon; D009161:Mycobacterium / Q000235:genetics; D009615:Nocardia / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D014031:Tobramycin / Q000494:pharmacology; D014169:Transformation, Bacterial / Q000235:genetics
D014031:Tobramycin