title: Mito-DEPMPO synthesized from a novel NH2-reactive DEPMPO spin trap: a new and improved trap for the detection of superoxide.
abstract: Mito-DEPMPO, a new DEPMPO analogue bearing a triphenylphosphonium group, was synthesized via a novel NH2-reactive DEPMPO. The half-life of the Mito-DEPMPO superoxide adduct was estimated to be ca. 40 min. Using Mito-DEPMPO, reactive oxygen species generated in intact mitochondria were detected and characterized by EPR.
authors.affiliation: SREP, UMR 6517 CNRS et Universités Aix-Marseille 1, 2 et 3, Centre de Saint Jérôme, 13013 Marseille, France.
authors.forename: Micael
authors.initials: M
authors.lastname: Hardy
authors.forename: Florence
authors.initials: F
authors.lastname: Chalier
authors.forename: Olivier
authors.initials: O
authors.lastname: Ouari
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Finet
authors.forename: Antal
authors.initials: A
authors.lastname: Rockenbauer
authors.forename: Balaraman
authors.initials: B
authors.lastname: Kalyanaraman
authors.forename: Paul
authors.initials: P
authors.lastname: Tordo
mesh_terms: D000818:Animals; D003497:Cyclic N-Oxides / Q000138:chemical synthesis* / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D019271:Hypoxanthine / Q000378:metabolism; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D010010:Osteoclasts / Q000166:cytology / Q000378:metabolism; D013113:Spin Labels; D019097:Spin Trapping; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*; D014969:Xanthine Oxidase / Q000378:metabolism
chemical_list: D003497:Cyclic N-Oxides; D013113:Spin Labels; D013481:Superoxides; C098505:5-diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide; D019271:Hypoxanthine; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase