title: Immunohistochemical marker panels for distinguishing between epithelioid mesothelioma and lung adenocarcinoma.
abstract: The distinction between epithelioid mesothelioma and lung adenocarcinoma remains an important diagnostic challenge for surgical pathologists. The aim of the present study was to select a limited and appropriate panel of antibodies that can differentiate between epithelioid mesothelioma and lung adenocarcinoma. Specimens of 90 epithelioid mesotheliomas and 51 lung adenocarcinomas obtained from Japanese cases were examined using calretinin, WT1, AE1/AE3, CAM5.2, cytokeratin (CK) 5/6, vimentin, epithelial membrane antigen (EMA), thrombomodulin, CEA, CA19-9, and CA125. Ninety-six percent of epithelioid mesotheliomas were positive for calretinin; 99% for WT1; 100% for AE1/AE; 97% for CAM5.2; 70% for CK 5/6; 91% for vimentin; 96% for EMA; 71% for thrombomodulin; 77% for mesothelin; 7% for CEA; 17% for CA19-9; and 85% for CA125. In contrast, 33% of lung adenocarcinomas were positive for calretinin; 16% for WT1; 100% for AE1/AE3, CAM5.2, and EMA; 41% for CK 5/6; 47% for vimentin; 20% for thrombomodulin; 69% for mesothelin; 98% for CEA; 73% for CA19-9; and 80% for CA125. For distinguishing between epithelioid mesothelioma and lung adenocarcinoma, the combination of CEA, calretinin and each WT1 or thrombomodulin was suggested to be the best panel of immunohistochemical markers.
authors.affiliation: Department of Pathology, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Kushitani
authors.forename: Yukio
authors.initials: Y
authors.lastname: Takeshima
authors.forename: Vishwa Jeet
authors.initials: VJ
authors.lastname: Amatya
authors.forename: Osamu
authors.initials: O
authors.lastname: Furonaka
authors.forename: Akio
authors.initials: A
authors.lastname: Sakatani
authors.forename: Kouki
authors.initials: K
authors.lastname: Inai
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis* / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000378:metabolism*; D064032:Calbindin 2; D002272:Carcinoembryonic Antigen / Q000032:analysis / Q000378:metabolism; D003937:Diagnosis, Differential; D015622:Epithelioid Cells / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D008654:Mesothelioma / Q000175:diagnosis* / Q000378:metabolism; D064030:S100 Calcium Binding Protein G / Q000032:analysis / Q000378:metabolism; D012680:Sensitivity and Specificity; D018180:Thrombomodulin / Q000032:analysis / Q000378:metabolism; D025721:WT1 Proteins / Q000032:analysis / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; C576764:CALB2 protein, human; D064032:Calbindin 2; D002272:Carcinoembryonic Antigen; D064030:S100 Calcium Binding Protein G; D018180:Thrombomodulin; D025721:WT1 Proteins