title: Pseudomonas delhiensis sp. nov., from a fly ash dumping site of a thermal power plant.
abstract: A phenanthrene- and citronellol-degrading bacterium, strain RLD-1(T), was isolated from the fly ash dumping site of a thermal power plant in Delhi, India. The 16S rRNA gene sequence indicated that this strain belongs to the genus Pseudomonas; high levels of sequence similarity were found with respect to Pseudomonas citronellolis DSM 50332(T) (98.9 %), Pseudomonas jinjuensis DSM 16612(T) (97.6 %) and Pseudomonas nitroreducens DSM 14399(T) (97.5 %). Phylogenetic analysis based on 16S rRNA gene sequences placed the strain within the clade represented by these three strains. Strain RLD-1(T) showed low levels of DNA-DNA hybridization with respect to P. citronellolis DSM 50332(T) (36 %), P. jinjuensis DSM 16612(T) (4 %) and P. nitroreducens (13.7 %). Strain RLD-1(T) can also be distinguished from these three strains on the basis of several biochemical and physiological attributes. The novel strain contained high levels of cellular fatty acids 18 : 1omega7c, 16 : 0 and 16 : 1omega7c, along with 10 : 0 3-OH and 12 : 0 3-OH. Thus, strain RLD-1(T) represents a novel species of the genus Pseudomonas, for which the name Pseudomonas delhiensis sp. nov. is proposed. The type strain is RLD-1(T) (=MTCC 7601(T)=CCM 7361(T)).
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi 110 007, India.
authors.forename: Om
authors.initials: O
authors.lastname: Prakash
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi 110 007, India.
authors.forename: Kirti
authors.initials: K
authors.lastname: Kumari
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi 110 007, India.
authors.forename: Rup
authors.initials: R
authors.lastname: Lal
mesh_terms: D000080462:Acyclic Monoterpenes; D002244:Carbon; D060729:Coal Ash; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D007194:India; D008969:Molecular Sequence Data; D039821:Monoterpenes / Q000378:metabolism; D052638:Particulate Matter; D010616:Phenanthrenes / Q000378:metabolism; D010802:Phylogeny; D011210:Power Plants; D011549:Pseudomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D000080462:Acyclic Monoterpenes; D060729:Coal Ash; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D039821:Monoterpenes; D052638:Particulate Matter; D010616:Phenanthrenes; D012336:RNA, Ribosomal, 16S; C031181:phenanthrene; D002244:Carbon; C007078:citronellol