title: Vaccination of horses against strangles using recombinant antigens from Streptococcus equi.
abstract: Strangles is an upper respiratory tract infection in horses, which is highly contagious and one of the more costly diseases of the horse. Three recombinant antigens were used to vaccinate horses, which were then experimentally challenged with Streptococcus equi, the causative agent for strangles. The vaccinated horses showed significantly reduced bacterial growth (p=0.02) and nasal discharge (p=0.0004), a typical symptom of strangles. Other clinical signs of strangles were also reduced and at post mortem examination, lower rate of empyaema or scarring of the guttural pouches was found in the vaccinated group (p=0.01). The antigens used were EAG (alpha2-macroglobulin, albumin, and IgG-binding protein), CNE (a collagen-binding protein), and SclC (a collagen-like protein). The adjuvant used was Abisco, a saponin derived matrix. No adverse effects were observed following vaccination with the antigens and adjuvant.
authors.affiliation: Animal Health Trust, Newmarket, UK.
authors.forename: Andrew
authors.initials: A
authors.lastname: Waller
authors.forename: Margareta
authors.initials: M
authors.lastname: Flock
authors.forename: Ken
authors.initials: K
authors.lastname: Smith
authors.forename: Carl
authors.initials: C
authors.lastname: Robinson
authors.forename: Zoe
authors.initials: Z
authors.lastname: Mitchell
authors.forename: Asa
authors.initials: A
authors.lastname: Karlström
authors.forename: Jonas
authors.initials: J
authors.lastname: Lannergård
authors.forename: Rune
authors.initials: R
authors.lastname: Bergman
authors.forename: Bengt
authors.initials: B
authors.lastname: Guss
authors.forename: Jan-Ingmar
authors.initials: JI
authors.lastname: Flock
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006734:Horse Diseases / Q000382:microbiology / Q000517:prevention & control*; D006736:Horses; D007074:Immunoglobulin G / Q000097:blood; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology*; D012141:Respiratory Tract Infections / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D013290:Streptococcal Infections / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary; D022541:Streptococcal Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D018502:Streptococcus equi / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D014611:Vaccination / Q000662:veterinary
chemical_list: D000942:Antigens, Bacterial; D007074:Immunoglobulin G; D008565:Membrane Proteins; D011994:Recombinant Proteins; D022541:Streptococcal Vaccines