An inhibitory role of nitric oxide in the dynamic regulation of the blood-brain barrier function.
1. The present study aimed at elucidating the effect of nitric oxide (NO) on blood-brain barrier (BBB) function with mouse brain capillary endothelial (MBEC4) cells.2. Histamine (20-100 microM) evoked NO production (1.6-7 microM) in MBEC4 cells in a dose-dependent manner.3. The permeability coefficient of sodium fluorescein for MBEC4 cells and the cellular accumulation of rhodamine 123 in MBEC4 cells were increased dose-dependently by the addition of NO solutions (14 and 28 microM) every 10 min during a 30-min period.4. The present study demonstrated that NO increased the permeability and inhibited the P-glycoprotein efflux pump of brain capillary endothelial cells, suggesting that NO plays an inhibitory role in the dynamic regulation of the BBB function.
Yamauchi
Atsushi
A
Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, Jonan-ku, Fukuoka 814-0180, Japan.
Dohgu
Shinya
S
Nishioku
Tsuyoshi
T
Shuto
Hideki
H
Naito
Mikihiko
M
Tsuruo
Takashi
T
Sawada
Yasufumi
Y
Kataoka
Yasufumi
Y
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism; D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects* / Q000502:physiology; D002463:Cell Membrane Permeability / Q000187:drug effects; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects; D004730:Endothelium, Vascular / Q000378:metabolism / Q000502:physiology; D019793:Fluorescein / Q000493:pharmacokinetics; D006632:Histamine / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000378:metabolism / Q000494:pharmacology* / Q000502:physiology*; D020112:Rhodamine 123 / Q000493:pharmacokinetics; D019108:Tight Junctions / Q000187:drug effects / Q000502:physiology
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D020112:Rhodamine 123; D009569:Nitric Oxide; D006632:Histamine; D019793:Fluorescein