title: Multilayered DNA coatings: in vitro bioactivity studies and effects on osteoblast-like cell behavior.
abstract: This study describes the effect of multilayered DNA coatings on (i) the formation of mineralized depositions from simulated body fluids (SBF); and (ii) osteoblast-like cell behavior with and without pretreatment in SBF. DNA coatings were generated using electrostatic self-assembly, with poly-d-lysine or poly(allylamine hydrochloride) as cationic polyelectrolytes, on titanium substrates. Coated substrates and non-coated controls were immersed in SBF with various compositions. The deposition of calcium phosphate was enhanced on multilayered DNA coatings as compared with non-coated controls, and was dependent on the type of cationic polyelectrolyte used in the build-up of the DNA coatings. Further analysis showed that the depositions consisted of carbonated apatite. Non-pretreated DNA coatings were found to have no effect on osteoblast-like cell behavior compared with titanium controls. On the other hand, SBF-pretreatment of DNA coatings affected the differentiation of osteoblast-like cells through an increased deposition of osteocalcin. The results of this study are indicative of the bone-bonding capacities of DNA coatings. Nevertheless, future animal experiments are required to provide conclusive evidence for the bioactivity of DNA coatings.
authors.affiliation: Department of Periodontology and Biomaterials, Radboud University Nijmegen Medical Center, Nijmegen, P.O. Box 9101, 6500 HB, The Netherlands.
authors.forename: J J J P
authors.initials: JJ
authors.lastname: van den Beucken
authors.forename: X F
authors.initials: XF
authors.lastname: Walboomers
authors.forename: S C G
authors.initials: SC
authors.lastname: Leeuwenburgh
authors.forename: M R J
authors.initials: MR
authors.lastname: Vos
authors.forename: N A J M
authors.initials: NA
authors.lastname: Sommerdijk
authors.forename: R J M
authors.initials: RJ
authors.lastname: Nolte
authors.forename: J A
authors.initials: JA
authors.lastname: Jansen
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001826:Body Fluids / Q000737:chemistry; D001854:Bone Marrow Cells / Q000166:cytology; D002130:Calcium Phosphates / Q000737:chemistry; D002244:Carbon / Q000737:chemistry; D002412:Cations / Q000737:chemistry; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003198:Computer Simulation; D004247:DNA / Q000737:chemistry* / Q000494:pharmacology; D017886:Durapatite / Q000737:chemistry; D004573:Electrolytes / Q000737:chemistry; D004577:Electron Probe Microanalysis; D005269:Femur / Q000166:cytology; D066298:In Vitro Techniques; D008297:Male; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology* / Q000648:ultrastructure; D015675:Osteocalcin / Q000032:analysis / Q000378:metabolism; D011108:Polymers / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D055672:Static Electricity; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry
chemical_list: D002130:Calcium Phosphates; D002412:Cations; D020099:Coated Materials, Biocompatible; D004573:Electrolytes; D011108:Polymers; D015675:Osteocalcin; D002244:Carbon; D004247:DNA; D017886:Durapatite; C020243:calcium phosphate; D014025:Titanium; D000469:Alkaline Phosphatase