Enhanced cutinase production with Thermobifida fusca by two-stage pH control strategy.
A mutant of Thermobifida fusca ATCC 27730 was used for cutinase production. Acetate was the most suitable carbon source for cell growth and cutinase production compared with others. The pH was one of the most important factors affecting cutinase yield and productivity. Batch cutinase fermentations by mutant Thermobifida fusca WSH04 at various pH values ranging from 7.0 to 7.9 were studied. Based on the effects of different pH values on the specific cell growth rate and specific cutinase formation rate, a two-stage pH control strategy was developed, in which the pH was set at 7.3 for the first 20 h, and switched to 7.6 afterwards. By applying this two-stage pH control strategy for cutinase fermentation, the maximal cutinase activity reached 19.8 U/mL.
Du
Guo-Cheng
GC
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Southern Yangtze University, Wuxi, China.
Zhang
Shou-Liang
SL
Hua
Zhao-Zhe
ZZ
Zhu
Yang
Y
Chen
Jian
J
D000192:Actinomycetales / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis*; D000431:Ethanol / Q000494:pharmacology; D005285:Fermentation / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology / Q000379:methods; D008563:Membrane Lipids / Q000378:metabolism; D009154:Mutation; D019346:Sodium Acetate / Q000494:pharmacology; D013213:Starch / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology
D008563:Membrane Lipids; D000431:Ethanol; D019346:Sodium Acetate; C000521:cutin; D013395:Sucrose; D013213:Starch; D002265:Carboxylic Ester Hydrolases; C019952:cutinase; D005947:Glucose