title: Study on the noncovalent complexes of ginsenoside and cytochrome c by electrospray ionization mass spectrometry.
abstract: The noncovalent complexes of cytochrome c and ginsenoside were studied by electrospray ionization mass spectrometry (ESI-MS). Ginsenoside Rb2 and Re were bound to cytochrome c to form several complexes with different stoichiometric relation. The 1:1 and 1:2 complexes of cytochrome c to ginsenoside were considered and the dissociation constants were obtained according to the intensities of cytochrome c and complexes when the concentrations of cytochrome c and ginsenoside have been known. Competition experiment was performed to validate the result. The K(D) values obtained with different reactive systems were consistent with each other.
authors.affiliation: College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012, PR China. analchem@mail.jlu.edu.cn
authors.forename: Huarong
authors.initials: H
authors.lastname: Zhang
authors.forename: Lan
authors.initials: L
authors.lastname: Ding
authors.forename: Chenling
authors.initials: C
authors.lastname: Qu
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Hanqi
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D045304:Cytochromes c / Q000737:chemistry*; D036145:Ginsenosides / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D036145:Ginsenosides; D046911:Macromolecular Substances; C048026:ginsenoside Rb2; C049864:ginsenoside Re; D045304:Cytochromes c