Exhaustive exercise, animal stress, and environmental hypercapnia on motility of sperm of steelhead trout (Oncorhynchus mykiss).
Motility of salmonid sperm is inhibited by the presence of carbon dioxide (CO2) in vitro; however, whether this occurs in response to challenges to the adult in vivo is not known. To determine whether CO2 negatively impacts sperm function in vivo, mature males were exposed to exhaustive exercise as well as to acute stress, chronic stress, tricaine anesthesia and environmental hypercapnia and sperm motility and semen CO2 tensions and pH values assessed. Semen CO2 rose and pH decreased significantly only in response to exhaustive exercise and environmental hypercapnia (13 kPa CO2). These changes in semen CO2 and pH were associated with reductions in numbers of sperm becoming motile upon water activation. Chronic and acute stress and tricaine anesthesia were without effect on sperm motility or on semen CO2 or pH. The time course of CO2 inhibition and recovery was evaluated in vitro. At least 50 min was required to note 50% of the inhibitory effect of low CO2 tensions on motility when sperm were exposed to 1.6-3.1 kPa CO2. At higher CO2 levels sperm motility displayed 50% of the inhibitory effect of these tensions within about 30 min. Sperm recovered maximal motility within 1 h of being placed in a nominally CO2-free environment. This study demonstrates sperm vulnerability to not only in vitro CO2 exposure but also in vivo exposure during exhaustive exercise and as result of environmental hypercapnia.
Zuccarelli
Micah D
MD
Department of Biological Sciences and Center for Reproductive Biology, University of Idaho, Moscow, ID 83844-3051, USA.
Ingermann
Rolf L
RL
D000086:Acetazolamide / Q000494:pharmacology; D000818:Animals; D002245:Carbon Dioxide / Q000097:blood / Q000494:pharmacology; D004777:Environment; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D006935:Hypercapnia / Q000139:chemically induced / Q000473:pathology*; D017686:Oncorhynchus mykiss / Q000502:physiology*; D010805:Physical Conditioning, Animal / Q000502:physiology*; D012661:Semen / Q000187:drug effects / Q000378:metabolism; D013081:Sperm Motility / Q000187:drug effects / Q000502:physiology*; D013312:Stress, Physiological / Q000503:physiopathology*; D013997:Time Factors
D002245:Carbon Dioxide; D000086:Acetazolamide