Advantages and limitation of BODIPY as a probe for the evaluation of lipid peroxidation and its inhibition by antioxidants in plasma.
Oxidative stress and the role of antioxidants are currently one of the most important subjects in the field of life science. In the present study, we assessed the oxidation of plasma lipids induced by free radicals and its inhibition by antioxidants with a fluorescence probe BODIPY. Vitamin E and C-depleted plasma was used to evaluate the inherent action of several antioxidants. BODIPY reacted with free radicals in plasma to emit fluorescence (ex. 510 nm, em. 520 nm), which was suppressed by the antioxidants in a concentration-dependent manner. However, the suppression of fluorescence emission by antioxidants did not always correlate quantitatively with the suppression of lipid peroxidation. For example, alpha-tocopherol suppressed BODIPY fluorescence but enhanced the peroxidation of plasma lipids in the absence of ascorbic acid. 2,2,5,7,8-Pentamethyl-6-chromanol, a vitamin E analogue without a phytyl side chain, almost completely suppressed both fluorescence emission and lipid peroxidation in the plasma. These results show that BODIPY can be used as a convenient probe for radical scavenging, but that care should be taken for the evaluation of antioxidant capacity.
Itoh
Nanako
N
National Institute of Advanced Industrial Science & Technology (AIST), Human Stress Signal Research Center, 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan.
Cao
Jiaofei
J
Chen
Zhi-Hua
ZH
Yoshida
Yasukazu
Y
Niki
Etsuo
E
D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D001896:Boron Compounds / Q000493:pharmacokinetics / Q000494:pharmacology*; D002352:Carrier Proteins / Q000235:genetics; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005609:Free Radicals; D015227:Lipid Peroxidation; D051379:Mice; D018345:Mice, Knockout; D008956:Models, Chemical; D008968:Molecular Conformation; D018384:Oxidative Stress; D051381:Rats; D024502:alpha-Tocopherol / Q000737:chemistry*
C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D000975:Antioxidants; D001896:Boron Compounds; D002352:Carrier Proteins; D016166:Free Radical Scavengers; D005609:Free Radicals; C017763:alpha-tocopherol transfer protein; D024502:alpha-Tocopherol