title: Polyelectrolyte complexes between (cross-linked) N-carboxyethylchitosan and (quaternized) poly[2-(dimethylamino)ethyl methacrylate]: preparation, characterization, and antibacterial properties.
abstract: Novel polyelectrolyte complexes (PECs) between N-carboxyethylchitosan (CECh) and well-defined (quaternized) poly[2-(dimethylamino)ethyl methacrylate] (PDMAEMA) have been obtained. The modification of chitosan into CECh allows the preparation of PECs in a pH range in which chitosan cannot form complexes. The CECh/PDMAEMA complex is formed in a narrow pH range around 7. The quaternization of the tertiary amino groups of PDMAEMA enables complex formation with CECh both in neutral and in alkaline medium. Cross-linked CECh is also capable of forming complexes with (quaternized) PDMAEMA. The antibacterial activity of (cross-linked) CECh, (quaternized) PDMAEMA, and their complexes against Escherichia coli has been evaluated. In contrast to (quaternized) PDMAEMA, (cross-linked) CECh exhibits no antibacterial activity. The complex formation between cross-linked CECh and (quaternized) PDMAEMA results in a loss of the inherent antibacterial activity of the latter in neutral medium. In acidic medium, the complexes exhibit strong antibacterial activity due to complex disintegration and release of (quaternized) PDMAEMA.
authors.affiliation: Laboratory of Bioactive Polymers, Institute of Polymers, Bulgarian Academy of Sciences, Acad. G. Bonchev Street Bl. 103A, 1113 Sofia, Bulgaria.
authors.forename: Elena
authors.initials: E
authors.lastname: Yancheva
authors.forename: Dilyana
authors.initials: D
authors.lastname: Paneva
authors.forename: Vera
authors.initials: V
authors.lastname: Maximova
authors.forename: Laetitia
authors.initials: L
authors.lastname: Mespouille
authors.forename: Philippe
authors.initials: P
authors.lastname: Dubois
authors.forename: Nevena
authors.initials: N
authors.lastname: Manolova
authors.forename: Iliya
authors.initials: I
authors.lastname: Rashkov
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D001671:Biochemistry / Q000379:methods; D002240:Carbohydrate Sequence; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004573:Electrolytes / Q000737:chemistry*; D004926:Escherichia coli / Q000378:metabolism; D004952:Esters / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D046911:Macromolecular Substances / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D008956:Models, Chemical; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D011108:Polymers / Q000737:chemistry; D019904:Polymethyl Methacrylate / Q000737:chemistry
chemical_list: D000890:Anti-Infective Agents; D003432:Cross-Linking Reagents; D004573:Electrolytes; D004952:Esters; D046911:Macromolecular Substances; C480500:N-carboxyethylchitosan ethyl ester; D011108:Polymers; D019904:Polymethyl Methacrylate; D048271:Chitosan