Identification of lactic acid bacteria isolated from human blood cultures.
Fifteen lactic acid bacterial strains were isolated from blood cultures from 15 different patients in the Faculty Hospital in Brno, Czech Republic. All strains were identified using biochemical tests and repetitive PCR using the (GTG)5 primer. Doubtful identification results were confirmed by whole-cell protein analysis. The strains were assigned to the genera Lactobacillus (eight strains representing seven species), Leuconostoc (six strains representing four species) and Weissella (one strain). Antibiotic susceptibility testing was performed using the E-test and revealed high-level resistance to cotrimoxazol, metronidazole, vancomycin and teicoplanin, but nearly all strains were susceptible to erythromycin, clindamycin, ampicillin and penicillin.
Svec
Pavel
P
Czech Collection of Microorganisms, Faculty of Science, Masaryk University, Brno, Czech Republic. mpavel@sci.muni.cz
Sevcíková
Alena
A
Sedlácek
Ivo
I
Bednárová
Jana
J
Snauwaert
Cindy
C
Lefebvre
Karen
K
Vandamme
Peter
P
Vancanneyt
Marc
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016470:Bacteremia / Q000382:microbiology*; D001426:Bacterial Proteins / Q000032:analysis; D015373:Bacterial Typing Techniques; D001769:Blood / Q000382:microbiology*; D002648:Child; D002675:Child, Preschool; D018153:Czech Republic; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D006094:Gram-Positive Bacteria / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology*; D006785:Hospitals, University; D006801:Humans; D007223:Infant; D007778:Lactobacillus / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D007934:Leuconostoc / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial