Colorimetric phage-based assay for detection of rifampin-resistant Mycobacterium tuberculosis.
Tests based on bacteriophage replication enable rapid screening of Mycobacterium tuberculosis for drug resistance. We describe a novel broth-based colorimetric method for detecting phage replication. When clinical isolates were tested by this novel method, high concordance was observed with both the traditional phage assay and gene mutation analysis for detection of resistance to rifampin.
McNerney
Ruth
R
Department of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, Keppel Street, London WC1E 7HT, United Kingdom. Ruth.Mcnerney@lshtm.ac.uk
Mallard
Kim
K
Urassa
Honorathy M R
HM
Lemma
Eshetu
E
Donoghue
Helen D
HD
D000904:Antibiotics, Antitubercular / Q000494:pharmacology*; D003124:Colorimetry; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D050296:Microbial Viability; D009160:Mycobacteriophages / Q000502:physiology; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000302:isolation & purification / Q000821:virology; D012293:Rifampin / Q000494:pharmacology*; D013223:Statistics as Topic; D014376:Tuberculosis / Q000382:microbiology; D010948:Viral Plaque Assay; D014779:Virus Replication
D000904:Antibiotics, Antitubercular; D004269:DNA, Bacterial; D012293:Rifampin