title: Unusual codon bias in vinyl chloride reductase genes of Dehalococcoides species.
abstract: Vinyl chloride reductases (VC-RDase) are the key enzymes for complete microbial reductive dehalogenation of chloroethenes, including the groundwater pollutants tetrachloroethene and trichloroethene. Analysis of the codon usage of the VC-RDase genes vcrA and bvcA showed that these genes are highly unusual and are characterized by a low G+C fraction at the third position. The third position of codons in VC-RDase genes is biased toward the nucleotide T, even though available Dehalococcoides genome sequences indicate the absence of any tRNAs matching codons that end in T. The comparatively high level of abnormality in the codon usage of VC-RDase genes suggests an evolutionary history that is different from that of most other Dehalococcoides genes.
authors.affiliation: Department of Civil and Environmental Engineering, James H Clark Center East Wing, E250A, Stanford University, Stanford, CA 94305-5429, USA.
authors.forename: Paul J
authors.initials: PJ
authors.lastname: McMurdie
authors.forename: Sebastian F
authors.initials: SF
authors.lastname: Behrens
authors.forename: Susan
authors.initials: S
authors.lastname: Holmes
authors.forename: Alfred M
authors.initials: AM
authors.lastname: Spormann
mesh_terms: D001482:Base Composition; D041862:Chloroflexi / Q000201:enzymology / Q000235:genetics*; D003062:Codon / Q000235:genetics*; D005798:Genes, Bacterial; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000235:genetics*; D012343:RNA, Transfer / Q000235:genetics; D014752:Vinyl Chloride / Q000378:metabolism*
chemical_list: D003062:Codon; D012343:RNA, Transfer; D010088:Oxidoreductases; D014752:Vinyl Chloride