title: [Comparison of direct and indirect environmental DNA extraction methods for samples from a high-temperature environment].
abstract: Two different direct DNA extraction methods and one indirect DNA extraction method were applied to recover environmental DNA from six mat samples, which were sampled closely around a high-temperature spring vent in Tengchong Rehai of the western Yunnan province. As the criteria, quantity and purity of the extracted crude DNA, the result of PCR amplification for denaturing gradient gel electrophoresis (DGGE) before and after crude DNA purification, and the Shannon-wiener index of DGGE profiles were used to evaluate the direct and indirect DNA extraction methods. For the samples of less biomass, the indirect method yielded available crude DNA with high purity, and the DNA was amplified without purification by PCR for DGGE. The two direct methods extracted less DNA than indirect method from the samples of less biomass, and the crude DNA extraction could not be amplified by PCR. Despite the lower quantity of DNA yield, the indirect DNA extraction method for the other' mat samples presented more diverse bacterial community than that of the direct DNA extraction methods according to the Shannon-wiener index of the PCR-DGGE profiles.
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, China. cliff.liu@163.com
authors.forename: Hong-wei
authors.initials: HW
authors.lastname: Liu
authors.forename: Qian
authors.initials: Q
authors.lastname: Peng
authors.forename: Qin-yuan
authors.initials: QY
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
authors.forename: Xiao-long
authors.initials: XL
authors.lastname: Cui
authors.forename: Lian-bing
authors.initials: LB
authors.lastname: Lin
authors.forename: Zhi-jian
authors.initials: ZJ
authors.lastname: Liu
authors.forename: Cheng-lin
authors.initials: CL
authors.lastname: Jiang
authors.forename: Li-hua
authors.initials: LH
authors.lastname: Xu
authors.forename: Yi-guang
authors.initials: YG
authors.lastname: Chen
authors.forename: Zhi-gang
authors.initials: ZG
authors.lastname: Wang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Ren
authors.forename: Lan
authors.initials: L
authors.lastname: Deng
authors.forename: Meng-liang
authors.initials: ML
authors.lastname: Wen
mesh_terms: D004269:DNA, Bacterial / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D004783:Environmental Microbiology; D045482:Hot Springs / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial