title: Development and validation of an RP-HPLC method for quantitative determination of vanillin and related phenolic compounds in Vanilla planifolia.
abstract: A simple and fast method was developed using RP-HPLC for separation and quantitative determination of vanillin and related phenolic compounds in ethanolic extract of pods of Vanilla planifolia. Ten phenolic compounds, namely 4-hydroxybenzyl alcohol, vanillyl alcohol, 3,4-dihydroxybenzaldehyde, 4-hydroxybenzoic acid, vanillic acid, 4-hydroxybenzaldehyde, vanillin, p-coumaric acid, ferulic acid, and piperonal were quantitatively determined using ACN, methanol, and 0.2% acetic acid in water as a mobile phase with a gradient elution mode. The method showed good linearity, high precision, and good recovery of compounds of interest. The present method would be useful for analytical research and for routine analysis of vanilla extracts for their quality control.
authors.affiliation: Natural Plant Products Division, Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, India. aksinha08@rediffmail.com
authors.forename: Arun Kumar
authors.initials: AK
authors.lastname: Sinha
authors.forename: Subash Chandra
authors.initials: SC
authors.lastname: Verma
authors.forename: Upendra Kumar
authors.initials: UK
authors.lastname: Sharma
mesh_terms: D001547:Benzaldehydes / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D010636:Phenols / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D031669:Vanilla / Q000737:chemistry*
chemical_list: D001547:Benzaldehydes; D010636:Phenols; C100058:vanillin