Lipoteichoic acid from Lactobacillus plantarum elicits both the production of interleukin-23p19 and suppression of pathogen-mediated interleukin-10 in THP-1 cells.
In this study, the stimulatory effects of different lactic acid bacteria strains, and their subcellular fractions, on the THP-1 cell line were evaluated. Lactobacillus plantarum was found in particular to induce high levels of IL-23p19 mRNA, but it moderately induced TNF-alpha production. IL-10 production was not entirely affected by L. plantarum stimulation. When subcellular fractions of L. plantarum were used to treat THP-1 cells, IL-23p19 mRNA expression was enhanced in a dose-responsive manner, specifically by lipoteichoic acid (LTA). The cotreatment of THP-1 cells by both L. plantarum and Staphylococcus aureus LTA resulted in decreased IL-10 production when compared with cells treated by S. aureus LTA alone. Taken together, these data suggest that LTA isolated from L. plantarum elicits stimulatory effects upon the expression of IL-23p19 and inhibitory effects on pathogen-mediated IL-10 production.
Kim
Han Geun
HG
School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Suwon, Korea.
Gim
Min Geun
MG
Kim
Joo Yun
JY
Hwang
Hyun Jin
HJ
Ham
Min Seok
MS
Lee
Jung Min
JM
Hartung
Thomas
T
Park
Jung Woo
JW
Han
Seung Hyun
SH
Chung
Dae Kyun
DK
D002460:Cell Line; D004306:Dose-Response Relationship, Immunologic; D015870:Gene Expression; D006801:Humans; D016753:Interleukin-10 / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D053760:Interleukin-23 Subunit p19 / Q000096:biosynthesis*; D048191:Lactiplantibacillus plantarum / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology*; D009000:Monocytes / Q000382:microbiology*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D013211:Staphylococcus aureus / Q000276:immunology; D013682:Teichoic Acids / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D053760:Interleukin-23 Subunit p19; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D013682:Teichoic Acids; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; C009900:lipoteichoic acid