title: [Sorting of spermatogonial stem cells in mice].
abstract: OBJECTIVE
  To search for an effective method for enriching spermatogonial stem cells in mice.
  
  
  METHODS
  Bilateral artificial cryptorchidism was performed on 20 six-week old male Kunming mice. Three months after the operation, the testes were removed and single cell suspension prepared by two-step enzyme digestion. FITC-conjugated anti-alpha6-integrin antibody and PE-conjugated anti-c-kit antibody were added for adequate time on ice. Then the cells with low side scatter light-scattering properties were sorted and positively stained for alpha6-integrin and negative c-kit expression. And the viability of the isolated cells was assessed by trypan blue exclusion.
  
  
  RESULTS
  The sorted spermatogonial stem cells constitute 2.8% of the testis cells and over 95% of them were viable.
  
  
  CONCLUSIONS
  FACS can be used to isolate quantities of viable spermatogonial stem cells.
authors.affiliation: Department of Urology, Renmin Hospital, Wuhan University, Wuhan, Hubei 430060, China.
authors.forename: Chun-xia
authors.initials: CX
authors.lastname: Liu
authors.forename: Ci
authors.initials: C
authors.lastname: Zhang
authors.forename: Ling-long
authors.initials: LL
authors.lastname: Wang
authors.forename: Xiao-xiang
authors.initials: XX
authors.lastname: Yu
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods*; D003456:Cryptorchidism; D004195:Disease Models, Animal; D005434:Flow Cytometry; D005453:Fluorescence; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011897:Random Allocation; D013093:Spermatogonia / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology*