Human papilloma virus prevalence in laryngeal squamous cell carcinoma.
OBJECTIVE
To determine the prevalence and type of human papilloma virus deoxyribonucleic acid (DNA) in cases of laryngeal squamous cell carcinoma.


METHODS
We analysed the prevalence of human papilloma virus infection in archived paraffin block specimens taken from 99 cases of laryngeal squamous cell carcinoma between 1990 and 2005, using polymerase chain reaction techniques. Biopsy specimens from five proven verrucous skin lesions were used as positive controls, and peripheral blood samples from five healthy volunteers were used as negative controls.


RESULTS
Four test samples were found to have inadequate deoxyribonucleic acid purity and were therefore excluded from the study. Human papilloma virus deoxyribonucleic acid was detected in seven of 95 cases of laryngeal squamous cell carcinoma (7.36 per cent). Human papilloma virus genotyping revealed double human papilloma virus infection in three cases and single human papilloma virus infection in the remaining four cases. The human papilloma virus genotypes detected were 6, 11 and 16 (the latter detected in only one case).


CONCLUSIONS
In our series, a very low human papilloma virus prevalence was found among laryngeal squamous cell carcinoma cases. The human papilloma virus genotypes detected were mostly 6 and/or 11, and 16 in only one case. To the best of our knowledge, this is the first report of human papilloma virus prevalence in laryngeal squamous cell carcinoma, based on polymerase chain reaction genotyping in a Turkish population.
Gungor
A
A
Department of Otolaryngology, Haydarpasa Educational Hospital, Istanbul, Turkey.
Cincik
H
H
Baloglu
H
H
Cekin
E
E
Dogru
S
S
Dursun
E
E
D052685:Alphapapillomavirus / Q000235:genetics / Q000302:isolation & purification*; D002294:Carcinoma, Squamous Cell / Q000821:virology*; D004279:DNA, Viral / Q000302:isolation & purification*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000821:virology*; D016133:Polymerase Chain Reaction; D014421:Turkey
D004279:DNA, Viral