High-throughput synthesis and analysis of acylated cyanohydrins.
The yields and optical purities of products obtained from chiral Lewis acid/Lewis base-catalysed additions of alpha-ketonitriles to prochiral aldehydes could be accurately determined by an enzymatic method. The amount of remaining aldehyde was determined after its reduction to an alcohol, whilst the two product enantiomers were analysed after subsequent hydrolysis first by the (S)-selective Candida antarctica lipase B and then by the unselective pig liver esterase. The method could be used for analysis of products obtained from a number of aromatic aldehydes and aliphatic ketonitriles. Microreactor technology was successfully combined with high-throughput analysis for efficient catalyst optimization.
Hamberg
Anders
A
School of Biotechnology, Department of Biochemistry, AlbaNova University Center, SE 106 91 Stockholm, Sweden.
Lundgren
Stina
S
Wingstrand
Erica
E
Moberg
Christina
C
Hult
Karl
K
D000818:Animals; D002384:Catalysis; D005656:Fungal Proteins; D008049:Lipase / Q000737:chemistry*; D009570:Nitriles / Q000138:chemical synthesis* / Q000737:chemistry*; D011084:Polycyclic Aromatic Hydrocarbons / Q000138:chemical synthesis* / Q000737:chemistry*; D013552:Swine
D005656:Fungal Proteins; D009570:Nitriles; D011084:Polycyclic Aromatic Hydrocarbons; D008049:Lipase; C431349:lipase B, Candida antarctica