title: N-terminal extension of Saccharomyces cerevisiae translation termination factor eRF3 influences the suppression efficiency of sup35 mutations.
abstract: The eukaryotic translation termination factor eRF3 stimulates release of nascent polypeptides from the ribosome in a GTP-dependent manner. In most eukaryotes studied, eRF3 consists of an essential, conserved C-terminal domain and a nonessential, nonconserved N-terminal extension. However, in some species, this extension is required for efficient termination. Our data show that the N-terminal extension of Saccharomyces cerevisiae eRF3 also participates in regulation of termination efficiency, but acts as a negative factor, increasing nonsense suppression efficiency in sup35 mutants containing amino acid substitutions in the C-terminal domain of the protein.
authors.affiliation: Department of Genetics, St Petersburg State University, St Petersburg, Russia.
authors.forename: Kirill
authors.initials: K
authors.lastname: Volkov
authors.forename: Kirill
authors.initials: K
authors.lastname: Osipov
authors.forename: Igor
authors.initials: I
authors.lastname: Valouev
authors.forename: Sergey
authors.initials: S
authors.lastname: Inge-Vechtomov
authors.forename: Ludmila
authors.initials: L
authors.lastname: Mironova
mesh_terms: D000483:Alleles; D015153:Blotting, Western; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D008958:Models, Molecular; D016254:Mutagenesis, Insertional; D010454:Peptide Termination Factors / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D011328:Prions / Q000235:genetics*; D014176:Protein Biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D010454:Peptide Termination Factors; D011328:Prions; C068068:SUP35 protein, S cerevisiae; C080775:SUP45 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins