RNA microarray for estimating relative abundance of 16S rRNA in microbial communities.
We developed an RNA microarray protocol in which total RNA from a microbial community was attached to a slide glass, and rRNA was detected by fluorescently labeled oligonucleotide probes. The RNA microarray requires only 4 h for hybridization and enables double staining and estimating relative abundance of rRNA.
Hoshino
Tatsuhiko
T
Department of Chemical Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku-ku, Tokyo 169-8555, Japan. hoshino@microbial-ecology.net
Furukawa
Kazuhiro
K
Tsuneda
Satoshi
S
Inamori
Yuhei
Y
D009606:Nitrosomonas / Q000235:genetics / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D010231:Paracoccus denitrificans / Q000235:genetics / Q000302:isolation & purification*; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D012722:Sewage / Q000382:microbiology*
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012722:Sewage