Factors influencing hemolytic activity of venom from the jellyfish Rhopilema esculentum Kishinouye.
In this study, hemolytic activity of venom from the jellyfish Rhopilema esculentum Kishinouye and some factors affecting it were assayed. The HU(50) of R. esculentum full venom (RFV) against chicken erythrocytes was 3.40 microg/ml and a Hill coefficient value was 1.73 suggesting at least two molecules participated in hemolytic activity. The hemolytic activity of RFV was affected by some chemical and physical factors such as divalent cations, EDTA, (NH(4))(2)SO(4), pH and temperature. In the presence of Mg(2+), Cu(2+), Zn(2+), Fe(2+), Ca(2+) (>or=2 mM), Mn(2+) ((>or=1 mM), EDTA ((>or=2 mM) and (NH(4))(2)SO(4), the hemolytic activity of RFV was reduced. RFV had strong hemolytic activity at the pH 6-10 and the hemolytic ratios were 0.95-1.19. Hemolytic activity was temperature-sensitive and when RFV was pre-incubated at temperatures over 40 degrees C, it was sharply reduced.
Yu
Huahua
H
Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China.
Li
Cuiping
C
Li
Ronggui
R
Xing
Ronge
R
Liu
Song
S
Li
Pengcheng
P
D000645:Ammonium Sulfate / Q000494:pharmacology; D000818:Animals; D002413:Cations, Divalent / Q000494:pharmacology; D002645:Chickens; D003064:Cnidarian Venoms / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004492:Edetic Acid / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects*; D006461:Hemolysis / Q000187:drug effects*; D053878:Hemolytic Agents / Q000633:toxicity*; D006863:Hydrogen-Ion Concentration; D011042:Poisons / Q000633:toxicity*; D013696:Temperature
D002413:Cations, Divalent; D003064:Cnidarian Venoms; D053878:Hemolytic Agents; D011042:Poisons; D004492:Edetic Acid; D000645:Ammonium Sulfate