title: [Biodiversity of acidophiles in the sediment at an acid mine drainage site].
abstract: A sediment sample was collected at an acid mine drainage site in Anhui. The acidophiles composition and community structure in the sediment was studied with 16S rDNA clone library and denaturing gradient gel electrophoresis (DGGE). The results indicated that the acidophiles in the sediment were novel compared with the data in GenBank. The acidophiles affiliated with Acidobacteria, beta/gamma-Proteobacteria, delta-Proteobacteria, Nitrospira, Candidate Division TM7, low G + C Gram-positive. delta-Proteobacteria were major group in this acidic ecosystem. The research of aicdophiles community in sediment can help us develop high efficient method to treat acid mine drainage (AMD).
authors.affiliation: Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. hao20709@yahoo.com.cn
authors.forename: Chun-Bo
authors.initials: CB
authors.lastname: Hao
authors.forename: Hong-Xun
authors.initials: HX
authors.lastname: Zhang
authors.forename: Zhi-Hui
authors.initials: ZH
authors.lastname: Bai
authors.forename: Bao-Guo
authors.initials: BG
authors.lastname: Zhang
authors.forename: Xu-Xiang
authors.initials: XX
authors.lastname: Zhang
mesh_terms: D042763:Acidithiobacillus / Q000302:isolation & purification; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D019015:Geologic Sediments / Q000382:microbiology*; D007220:Industrial Waste / Q000032:analysis; D008906:Mining; D010802:Phylogeny; D020560:Proteobacteria / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013455:Sulfur; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000032:analysis
chemical_list: D007220:Industrial Waste; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; D013455:Sulfur