Nitric oxide accelerates the recovery from burn wounds.
A full-thickness burn wound model was used to evaluate the effects of a topically applied gel-based nitric oxide donor on wound healing in rats. The histological study demonstrated that the nitric oxide (NO) application significantly promoted re-epithelization that resulted in a fast recovery of burn wound. The histological sections further revealed that inflammatory cell infiltration in the NO-treated group was significantly increased in comparison to the control group. The enhanced accumulation of inflammatory cells resulted in a higher expression of myeloperoxidase (MPO) that was detected with imunoblotting. An immunohistochemistry study with CD31, a specific marker for endothelial cells, indicated that NO treatment markedly stimulated angiogenesis. Evaluation of collagen synthesis by immunohistochemistry with procollagen antibody demonstrated a significantly increased collagen synthesis in NO-treated wound bed. We concluded that NO treatment promoted re-epithelialization and wound closure by means of enhanced inflammatory cell infiltration, and that it promoted angiogenesis and facilitated collagen synthesis in the wound bed.
Zhu
Haifeng
H
Department of Integrative Biology and Pharmacology, Institute of Molecular Medicine, The University of Texas-Health Science Center at Houston, 6431 Fannin St, Houston, Texas 77030, USA.
Ka
Bian
B
Murad
Ferid
F
D000287:Administration, Topical; D000818:Animals; D015153:Blotting, Western; D002056:Burns / Q000188:drug therapy*; D004195:Disease Models, Animal; D005782:Gels; D007124:Immunoenzyme Techniques; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects; D009569:Nitric Oxide / Q000494:pharmacology*; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012977:Sodium Nitrite / Q000008:administration & dosage / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*
D005782:Gels; D009569:Nitric Oxide; D009195:Peroxidase; D012977:Sodium Nitrite