High expression of co-stimulatory and adhesion molecules are observed on eosinophils during human Schistosoma mansoni infection.
Herein we have focused attention on major phenotypic features of peripheral blood eosinophils from chronic Schistosoma mansoni-infected patients. For this purpose, detailed immunophenotypic profiles of a range of cell surface markers were performed, including activation markers (CD23/CD69/CD25/HLA-DR), co-stimulatory molecules (CD28/CD80/CD86), chemokine receptors (CXCR1/CXCR2/CCR3/CCR5) besides L-selectin-CD62L and adhesion molecules (CD18/CD54). Our major findings pointed out increased frequency of CD23+-cells, besides decreased percentages of CD69+-eosinophils, suggesting a chronic activation status with low frequency of early activated eosinophils in chronic S. mansoni-infected patients (INT) in comparison to non-infected individuals (NI). Moreover, a dichotomic expression of beta-chemokine receptors was observed during human schistosomiasis mansoni with higher CCR5 and lower levels of CCR3 observed between groups. Enhanced expression of co-stimulatory receptors (CD28/CD86) and adhesion molecules (CD54/CD18), besides striking lower frequency of L-selectin+ were reported for eosinophils from INT group as compared to NI. Interestingly, the frequency of CD62L+-eosinophils and a range of cell activation related molecules pointed out an opposite pattern of association in NI and INT, where only INT patients that display lower frequency of CD62L+-eosinophils (first CD62L tertile) kept the unusual relationship between the expression of L-selectin and the CD23 activation marker. These findings suggest that distinct dynamic of activation markers expressed by eosinophils may occur during chronic S. mansoni infection.
Silveira-Lemos
Denise
D
Laboratório de Imunologia Celular e Molecular, Centro de Pesquisas René-Rachou-Fiocruz, Av. Augusto de Lima 1715, 31190-002 Belo Horizonte, MG, Brazil.
Teixeira-Carvalho
Andréa
A
Martins-Filho
Olindo Assis
OA
Oliveira
Lúcia Fraga Alves
LF
Corrêa-Oliveira
Rodrigo
R
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D016022:Case-Control Studies; D015815:Cell Adhesion Molecules / Q000276:immunology*; D002908:Chronic Disease; D004802:Eosinophilia / Q000276:immunology*; D004804:Eosinophils / Q000276:immunology*; D006801:Humans; D016130:Immunophenotyping; D008875:Middle Aged; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000276:immunology*
D015815:Cell Adhesion Molecules