Safety evaluation of a heavy oil-degrading bacterium, Rhodococcus erythropolis C2.
The safety of an oil-degrading bacterium, C2 strain, was evaluated for utilization in an open system for bioremediation of oil-contaminated environments. The C2 strain was identified as Rhodococcus erythropolis by performing an alignment analysis of the whole 16S rRNA sequence. R. erythropolis was classified as a nonpathogenic (category 1) bacterium. Biological and biochemical properties of the C2 strain also confirmed its nonpathogenicity. The pathogenicity and basic ecotoxicity were studied in laboratory animals and in a variety of test species, respectively. General and inhalation toxicities were not detected; additionally, there was no evidence of skin irritation, mutagenic potential, eye irritation, skin sensitization, ecotoxicity or notable pathogenicity. The comparison of these results with human exposure levels and previously published data indicates that the C2 strain appears to be safe for utilization in bioremediation of polluted environments, requires no special occupational health precautions during the application process, and has a low environmental impact. This study suggests that the C2 strain could be suitable for bioremediation of oil-contaminated environments.
Aoshima
Hisae
H
KRI Inc., Kyoto Research Park, 134 Chudoji-minami-machi, Shimogyo-ku, Kyoto 600-8813, Japan. hisae.aoshima@vc60.com
Hirase
Tatsuaki
T
Tada
Takakiyo
T
Ichimura
Naoya
N
Kato
Hiroyuki
H
Nagata
Yohei
Y
Myoenzono
Takeshi
T
Taguchi
Mitsuru
M
Takahashi
Kazuo
K
Hukuzumi
Takao
T
Aoki
Takuya
T
Makino
Seiichi
S
Hagiya
Kozo
K
Ishiwata
Hiroyuki
H
D000818:Animals; D001673:Biodegradation, Environmental; D004785:Environmental Pollutants / Q000378:metabolism; D005260:Female; D006168:Guinea Pigs; D008297:Male; D016296:Mutagenesis; D010578:Petroleum; D011817:Rabbits; D051381:Rats; D012240:Rhodococcus / Q000737:chemistry / Q000472:pathogenicity*; D012449:Safety
D004785:Environmental Pollutants; D010578:Petroleum