Abl-SH3 binding protein 2, 3BP2, interacts with CIN85 and HIP-55.
The adapter 3BP2 is involved in leukocyte signaling downstream Src/Syk-kinases coupled immunoreceptors. Here, we show that 3BP2 directly interacts with the endocytic scaffold protein CIN85 and the actin-binding protein HIP-55. 3BP2 co-localized with CIN85 and HIP-55 in T cell rafts and at the T cell/APC synapse, an active zone of receptors and proteins recycling. A binding region of CIN85 SH3 domains on 3BP2 was mapped to a PVPTPR motif in the first proline-rich region of 3BP2, whereas the C-terminal SH3 domain of HIP-55 bound a more distal proline-rich domain of 3BP2. Together, our data suggest an unexpected role of 3BP2 in endocytic and cytoskeletal regulation through its interaction with CIN85 and HIP-55.
Le Bras
Séverine
S
Institut National de la Santé et de la Recherche Médicale, Université de Nice-Sophia-Antipolis, UMR 576, Hôpital de l'Archet, Route Saint Antoine de Ginestière, Nice cédex 3, F-06202, France.
Moon
Cheol
C
Foucault
Isabelle
I
Breittmayer
Jean-Philippe
JP
Deckert
Marcel
M
D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001665:Binding Sites; D002460:Cell Line; D003599:Cytoskeleton / Q000378:metabolism; D004705:Endocytosis; D006801:Humans; D066298:In Vitro Techniques; D019169:Jurkat Cells; D008840:Microfilament Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008954:Models, Biological; D011392:Proline / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D018909:src Homology Domains / Q000235:genetics
D048868:Adaptor Proteins, Signal Transducing; C117063:DBNL protein, human; D008840:Microfilament Proteins; D011994:Recombinant Proteins; C109073:SH3BP2 protein, human; C433810:SH3KBP1 protein, human; D011392:Proline