A small heat shock protein of Ostertagia ostertagi: stage-specific expression, heat inducibility, and protection trial.
In this study, we isolated and analyzed a small heat shock protein (HSP) of Ostertagia ostertagi (Oo-HSP18). Oo-hsp18 is encoded by a single-copy gene and the full-length cDNA represents an 18-kDa protein. The expression of Oo-hsp18 is highly stage specific and restricted to the adult stage. The protein is synthesized in a tissue-specific manner and localized in the body muscle layer. The levels of Oo-hsp18 mRNAs are sharply induced by heat shock but not by other stressors such as levamisole and H2O2. A vaccination trial with recombinant Oo-HSP18 failed to protect calves against a challenge infection.
Vercauteren
Isabel
I
Laboratory of Parasitology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan, 133, B-9820 Merelbeke, Belgium.
De Maere
Veerle
V
Vercruysse
Jozef
J
Stevens
Mieke
M
Gevaert
Kris
K
Claerebout
Edwin
E
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D018509:DNA, Helminth / Q000737:chemistry / Q000302:isolation & purification; D015870:Gene Expression; D050888:Heat-Shock Proteins, Small / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D006358:Hot Temperature; D008297:Male; D008969:Molecular Sequence Data; D010028:Ostertagia / Q000235:genetics / Q000276:immunology*; D010029:Ostertagiasis / Q000517:prevention & control / Q000662:veterinary; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D018411:Spodoptera; D014158:Transcription, Genetic
D018076:DNA, Complementary; D018509:DNA, Helminth; D050888:Heat-Shock Proteins, Small; D011994:Recombinant Proteins