Specific role of mitochondrial electron transport in blood-stage Plasmodium falciparum.
The origin of all mitochondria can be traced to the symbiotic arrangement that resulted in the emergence of eukaryotes in a world that was exclusively populated by prokaryotes. This arrangement, however, has been in continuous genetic flux: the varying degrees of gene loss and transfer from the mitochondrial genome in different eukaryotic lineages seem to signify an ongoing 'conflict' between the host and the symbiont. Eukaryotic parasites belonging to the phylum Apicomplexa provide an excellent example to support this view. These organisms contain the smallest mitochondrial genomes known, with an organization that differs among various genera; one genus, Cryptosporidium, seems to have lost the entire mitochondrial genome. Here we show that erythrocytic stages of the human malaria parasite Plasmodium falciparum seem to maintain an active mitochondrial electron transport chain to serve just one metabolic function: regeneration of ubiquinone required as the electron acceptor for dihydroorotate dehydrogenase, an essential enzyme for pyrimidine biosynthesis. Transgenic P. falciparum parasites expressing Saccharomyces cerevisiae dihydroorotate dehydrogenase, which does not require ubiquinone as an electron acceptor, were completely resistant to inhibitors of mitochondrial electron transport. Maintenance of mitochondrial membrane potential, however, was essential in these parasites, as indicated by their hypersensitivity to proguanil, a drug that collapsed the membrane potential in the presence of electron transport inhibitors. Thus, acquisition of just one enzyme can render mitochondrial electron transport nonessential in erythrocytic stages of P. falciparum.
Painter
Heather J
HJ
Center for Molecular Parasitology, Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19129, USA.
Morrisey
Joanne M
JM
Mather
Michael W
MW
Vaidya
Akhil B
AB
D000818:Animals; D030801:Animals, Genetically Modified; D053626:Atovaquone / Q000494:pharmacology; D000090722:Dihydroorotate Dehydrogenase; D004579:Electron Transport / Q000187:drug effects; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000235:genetics / Q000378:metabolism; D010963:Plasmodium falciparum / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D002727:Proguanil / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D014451:Ubiquinone / Q000378:metabolism
D000090722:Dihydroorotate Dehydrogenase; D014451:Ubiquinone; D044925:Oxidoreductases Acting on CH-CH Group Donors; D002727:Proguanil; D053626:Atovaquone