C3b complexation diversifies naturally processed T cell epitopes.
In addition to its well-established role in innate immunity, the complement component C3 is of critical importance in modulating the humoral response. In this study, we examined the effect of C3b linkage to tetanus toxin (TeNT) in the production of antigenic peptides inside human APC. We purified HLA-DR associated peptides isolated either from TeNT or TeNT-C3b pulsed cells. This study revealed that TeNT-C3b derived antigenic peptides are different and more numerous than TeNT derived peptides. This increased production of antigenic peptides correlated with a C3b-induced TeNT modification of proteolysis. These findings argue in favour of a new role for C3b in the modulation of T cell epitope during antigen processing and presentation.
Cretin
François C
FC
Laboratoire d'Immunochimie, Département Réponse et Dynamique Cellulaires, Commissariat à l'Energie Atomique-Grenoble, INSERM Unité 548, Université Joseph Fourier Grenoble, 17 rue des Martyrs, 38054 Grenoble Cedex, France.
Serra
Vincent A
VA
Villiers
Marie-Bernadette
MB
Laharie
Anne-Marie
AM
Marche
Patrice N
PN
Gabert
Françoise M
FM
D000818:Animals; D017951:Antigen Presentation; D002460:Cell Line; D003179:Complement C3b / Q000276:immunology*; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D007113:Immunity, Innate; D008213:Lymphocyte Activation; D013744:Tetanus Toxin / Q000276:immunology
D018984:Epitopes, T-Lymphocyte; D006684:HLA-DR Antigens; D013744:Tetanus Toxin; D003179:Complement C3b