Insulin resistance following thermal injury: an animal study.
The aim of this study was to investigate the changes of glucose tolerance, insulin sensitivity, and euglycemic-hyperinsulinemic glucose clamps following a 30% TBSA full thickness third degree burn in rats. Sprague-Dawley rats weighing 160-170g received 30% TBSA full thickness third degree burn by immersing the back of trunk for 12s in a boiling water bath under anesthesia. Weight- and time-matched sham burn group (control) was treated in the same manner as the trauma group, except that they were immersed in a room-temperature water bath. After 12h overnight fasting, plasma insulin concentration was determined by ELISA using rat-insulin enzyme immunoassay kit (SPI-BIO) and blood glucose was assayed by glucose analyzer at 3 days after burn. Insulin sensitivity index was calculated by using slightly modified formula. The rat was injected with 5% glucose (2g/kg body weight, intraperitoneally) to observe the change of glucose tolerance at 3 days after burn. Euglycemic-hyperinsulinemic glucose clamps were performed at 4 days after burn. Insulin sensitivity index of burn group was significantly reduced compared with control group at 3 days after burn (0.58+/-0.23 versus 1.23+/-0.16, P<0.01). The significant difference of glucose tolerance was observed between the two groups and the glucose infused rate measured in burned rats was significantly decreased compared with that in control at 4 days after injury (7.23+/-1.35 versus 12.31+/-0.54, P<0.01).


CONCLUSIONS
Burn causes the significant change of glucose metabolism and results in insulin resistance in rats.
Xin-Long
Chen
C
Burn Center, Changhai Hospital, Second Military Medical University, Shanghai 200433, People's Republic of China; Department of Burn, Pingjin Hospital, 300162 Tianjin, People's Republic of China.
Zhao-Fan
Xia
X
Dao-Feng
Ben
B
Jian-Guang
Tian
T
Duo
Wei
W
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D002056:Burns / Q000097:blood*; D015309:Glucose Clamp Technique; D005951:Glucose Tolerance Test; D007004:Hypoglycemic Agents / Q000008:administration & dosage; D007328:Insulin / Q000008:administration & dosage / Q000378:metabolism*; D007333:Insulin Resistance / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D001786:Blood Glucose; D007004:Hypoglycemic Agents; D007328:Insulin