title: Improved synthesis methods of standards used for quantitative determination of total isothiocyanates from broccoli in human urine.
abstract: A well-known method for quantification of isothiocyanates (ITCs) and their metabolites is the condensation reaction with 1,2-benzenedithiole to produce 1,3-benzodithiole-2-thione, which can be quantified by high-performance liquid chromatography. Standards of an ITC metabolite and 1,3-benzodithiole-2-thione are required for this assay but are not commercially available. In the present study, we report on an improved synthesis of the ITC metabolite N-acetyl-S-(N-4-methylsulfinylbutylthiocarbamoyl)-L-cysteine and 1,3-benzodithiole-2-thione. The standards were used to quantify the urinary excretion of ITCs from 10 healthy subjects who consumed 350 g broccoli. The excretion was investigated throughout 48 h showing a cumulative urinary ITC excretion of 49.1+/-25.2% of the dose.
authors.affiliation: Department of Toxicology and Risk Assessment, Danish Institute for Food and Veterinary Research, 2860 Søborg, Denmark. mehxy@dfvf.dk
authors.forename: Mette
authors.initials: M
authors.lastname: Kristensen
authors.forename: Kirstine S
authors.initials: KS
authors.lastname: Krogholm
authors.forename: Hanne
authors.initials: H
authors.lastname: Frederiksen
authors.forename: Fritz
authors.initials: F
authors.lastname: Duus
authors.forename: Claus
authors.initials: C
authors.lastname: Cornett
authors.forename: Susanne H
authors.initials: SH
authors.lastname: Bügel
authors.forename: Salka E
authors.initials: SE
authors.lastname: Rasmussen
mesh_terms: D000328:Adult; D001937:Brassica / Q000737:chemistry*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D006801:Humans; D017879:Isothiocyanates / Q000592:standards / Q000652:urine*; D008297:Male; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet
chemical_list: D017879:Isothiocyanates