Identification of acid-sensing ion channels in adenoid cystic carcinomas.
Tissue acidosis is an important feature of tumor. The response of adenoid cystic carcinoma (ACC) cells to acidic solution was studied using whole-cell patch-clamp recording in the current study. An inward, amiloride-sensitive Na(+) current was identified in cultured ACC-2 cells while not in normal human salivary gland epithelial cells. Electrophysiological and pharmacological properties of the currents suggest that heteromeric acid-sensing ion channels (ASICs) containing 2a and 3 may be responsible for the proton-induced currents in the majority of ACC-2 cells. Consistent with it, analyses of RT-PCR and Western blotting demonstrated the presences of ASIC2a and 3 in ACC-2 cells. Furthermore, we observed the enhanced expression of ASIC2a and 3 in the sample of ACC tissues. These results indicate that the functional expression of ASICs is characteristic feature of ACC cells.
Ye
Jin-Hai
JH
Department of Oral and Maxillofacial Surgery, School of Stomatology, Affiliated Ninth People's Hospital, Shanghai Jiaotong University, Shanghai Research Institute of Stomatology, Shanghai 200011, China.
Gao
Jun
J
Wu
Yu-Nong
YN
Hu
Yong-Jie
YJ
Zhang
Chen-Ping
CP
Xu
Tian-Le
TL
D062565:Acid Sensing Ion Channels; D003528:Carcinoma, Adenoid Cystic / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D004594:Electrophysiology; D005786:Gene Expression Regulation; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D011522:Protons; D012469:Salivary Glands / Q000378:metabolism; D015222:Sodium Channels / Q000235:genetics / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism
D062565:Acid Sensing Ion Channels; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D011522:Protons; D015222:Sodium Channels; D015032:Zinc