N-3 PUFAs modulate global gene expression profile in cultured rat cardiomyocytes. Implications in cardiac hypertrophy and heart failure.
In cardiac cells the effects of n-3 PUFAs on the whole genome are still unknown despite their recognized cardioprotective effects and ability to modulate gene expression. We have evaluated the effects of n-3 PUFAs supplementation on the global gene expression profile in cultured neonatal rat cardiomyocytes, detecting many genes related to lipid transport and metabolism among the upregulated ones. Many of the downregulated genes appeared related to inflammation, cell growth, extracellular and cardiac matrix remodelling, calcium movements and ROS generation. Our data allow to speculate that the cardioprotective effect of n-3 PUFAs is related to a direct modulation of genes in cardiac cells.
Bordoni
Alessandra
A
Nutrition Research Center, Department of Biochemistry "G. Moruzzi", University of Bologna, Via Irnerio, 48 - 40126 Bologna, Italy. alessandra.bordoni@unibo.it
Astolfi
Annalisa
A
Morandi
Luca
L
Pession
Andrea
A
Danesi
Francesca
F
Di Nunzio
Mattia
M
Franzoni
Monica
M
Biagi
Pierluigi
P
Pession
Annalisa
A
D000818:Animals; D001483:Base Sequence; D006332:Cardiomegaly / Q000235:genetics / Q000517:prevention & control; D002316:Cardiotonic Agents / Q000494:pharmacology; D002478:Cells, Cultured; D017931:DNA Primers / Q000235:genetics; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D006333:Heart Failure / Q000235:genetics / Q000517:prevention & control; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism*; D051381:Rats
D002316:Cardiotonic Agents; D017931:DNA Primers; D015525:Fatty Acids, Omega-3