title: Dissociaton of conditioned eye and limb responses in the cerebellar interpositus.
abstract: Thompson and colleagues have demonstrated that the lateral interpositus nucleus of the cerebellum is the essential locus for the classical conditioning of the somatic eyeblink response. Preliminary studies reported that lesioning the cerebellar interpositus nucleus ipsilateral to the side of training also appears to abolish conditioned limb flexion responses. Previous studies have suggested that the interpositus nucleus is somatotopically organized with the eye being represented laterally and the hindlimb medially. Presently, we employed a double dissociation paradigm to examine the effects of muscimol (a GABA(A) agonist) injections on eyeblink versus limb flexion conditioned responses in the ipsilateral cerebellar interpositus nucleus of New Zealand white rabbits. For eyeblink conditioning, the conditioned stimulus (CS) was a 14-V lamp bulb and the unconditioned stimulus (US) was a 3-psi corneal airpuff to the left eye. For limb flexion conditioning, the CS was a 1-kHz, 85-95 dB SPL tone and the US was a 3- to 5-mA shock to the upper left hindlimb. Upon training on both responses to a 60-100% criterion, the rabbits were then tested on eyeblink and limb flexion responses after injections of muscimol (0.1-0.3 mul of a 0.01- to 1.0-M solution) into either the lateral (eyeblink) or medial (limb flexion) interpositus nucleus. We have been able to successfully decrease or abolish the percent conditioned responses (CRs) of both the eyeblink and limb flexion conditioning selectively without affecting the other. These results thus lend further support for the notion of the existence of a somatotopic map in the interpositus nucleus for learning.
authors.affiliation: Program in Neuroscience, University Southern California, Los Angeles, CA 90089-2529, USA. smojtahedian@innovneurotech.com
authors.forename: S
authors.initials: S
authors.lastname: Mojtahedian
authors.forename: D R
authors.initials: DR
authors.lastname: Kogan
authors.forename: S A
authors.initials: SA
authors.lastname: Kanzawa
authors.forename: R F
authors.initials: RF
authors.lastname: Thompson
authors.forename: D G
authors.initials: DG
authors.lastname: Lavond
mesh_terms: D000818:Animals; D001931:Brain Mapping; D002529:Cerebellar Nuclei / Q000187:drug effects / Q000502:physiology*; D003215:Conditioning, Eyelid / Q000187:drug effects / Q000502:physiology*; D007839:Functional Laterality / Q000502:physiology; D018755:GABA Agonists / Q000008:administration & dosage / Q000494:pharmacology; D058785:GABA-A Receptor Agonists; D006614:Hindlimb / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008845:Microinjections; D009118:Muscimol / Q000008:administration & dosage / Q000494:pharmacology; D011817:Rabbits; D012018:Reflex / Q000187:drug effects / Q000502:physiology
chemical_list: D018755:GABA Agonists; D058785:GABA-A Receptor Agonists; D009118:Muscimol