title: The role of c-AMP-dependent protein kinase in spinal cord and post synaptic dorsal column neurons in a rat model of visceral pain.
abstract: Visceral noxious stimulation induces central neuronal plasticity changes and suggests that the c-AMP-dependent protein kinase (PKA) signal transduction cascade contributes to long-term changes in nociceptive processing at the spinal cord level. Our previous studies reported the clinical neurosurgical interruption of post synaptic dorsal column neuron (PSDC) pathway by performing midline myelotomy effectively alleviating the intractable visceral pain in patients with severe pain. However, the intracellular cascade in PSDC neurons mediated by PKA nociceptive neurotransmission was not known. In this study, by using multiple experimental approaches, we investigated the role of PKA in nociceptive signaling in the spinal cord and PSDC neurons in a visceral pain model in rats with the intracolonic injection of mustard oil. We found that mustard oil injection elicited visceral pain that significantly changed exploratory behavior activity in rats in terms of decreased numbers of entries, traveled distance, active and rearing time, rearing activity and increased resting time when compared to that of rats receiving mineral oil injection. However, the intrathecal infusion of PKA inhibitor, H89 partially reversed the visceral pain-induced effects. Results from Western blot studies showed that mustard oil injection significantly induced the expression of PKA protein in the lumbosacral spinal cord. Immunofluorescent staining in pre-labeled PSDC neurons showed that mustard oil injection greatly induces the neuronal profile numbers. We also found that the intrathecal infusion of a PKA inhibitor, H89 significantly blocked the visceral pain-induced phosphorylation of c-AMP-responsive element binding (CREB) protein in spinal cord in rats. The results of our study suggest that the PKA signal transduction cascade may contribute to visceral nociceptive changes in spinal PSDC pathways.
authors.affiliation: Department of Neurology, University of Texas Health Science Center, Houston, TX 77030, United States.
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.forename: Guangxiao
authors.initials: G
authors.lastname: Su
authors.forename: Long
authors.initials: L
authors.lastname: Ma
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhang
authors.forename: Yongzhong
authors.initials: Y
authors.lastname: Lei
authors.forename: Qing
authors.initials: Q
authors.lastname: Lin
authors.forename: Haring J W
authors.initials: HJ
authors.lastname: Nauta
authors.forename: Junfa
authors.initials: J
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Fang
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D015153:Blotting, Western; D002408:Catheters, Indwelling; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D004195:Disease Models, Animal; D007278:Injections, Spinal; D007546:Isoquinolines / Q000494:pharmacology; D008297:Male; D009149:Mustard Plant; D009474:Neurons / Q000737:chemistry / Q000378:metabolism; D010146:Pain / Q000378:metabolism*; D010938:Plant Oils / Q000008:administration & dosage; D047428:Protein Kinase Inhibitors / Q000008:administration & dosage; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D013116:Spinal Cord / Q000378:metabolism*; D013449:Sulfonamides / Q000494:pharmacology
chemical_list: D007546:Isoquinolines; D010938:Plant Oils; D047428:Protein Kinase Inhibitors; D013449:Sulfonamides; D017868:Cyclic AMP-Dependent Protein Kinases; C063509:N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide; C027793:mustard oil