title: Apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G: a possible role in the resistance to HIV of HIV-exposed seronegative individuals.
abstract: Apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G (APOBEC3G), a human cytidine deaminase, is a potent inhibitor of HIV replication. To explore a possible role of this protein in modulating in vivo susceptibility to HIV infection, we analyzed APOBEC3G expression in HIV-exposed seronegative individuals, HIV-seropositive patients, and healthy control subjects. The results showed that the expression of APOBEC3G is significantly increased in peripheral blood mononuclear cells (PBMCs)--mainly CD14(+) cells--and in cervical tissues of HIV-exposed seronegative individuals. Higher APOBEC3G expression correlated with a reduced susceptibility of PBMCs to in vitro infection with the HIV-1(Ba-L) R5 strain. APOBEC3G could be important in modulating in vivo susceptibility to sexually transmitted HIV infection.
authors.affiliation: Department of Immunology, Dipartimento di Scienze Precliniche Laboratorio Interdisciplinare Tecnologie Avanzate Vialba, Milano University Medical School, Milano, Italy.
authors.forename: Mara
authors.initials: M
authors.lastname: Biasin
authors.forename: Luca
authors.initials: L
authors.lastname: Piacentini
authors.forename: Sergio
authors.initials: S
authors.lastname: Lo Caputo
authors.forename: Yasuyoshi
authors.initials: Y
authors.lastname: Kanari
authors.forename: Giuliana
authors.initials: G
authors.lastname: Magri
authors.forename: Daria
authors.initials: D
authors.lastname: Trabattoni
authors.forename: Valentina
authors.initials: V
authors.lastname: Naddeo
authors.forename: Lucia
authors.initials: L
authors.lastname: Lopalco
authors.forename: Alberto
authors.initials: A
authors.lastname: Clivio
authors.forename: Eugenio
authors.initials: E
authors.lastname: Cesana
authors.forename: Francesca
authors.initials: F
authors.lastname: Fasano
authors.forename: Cristina
authors.initials: C
authors.lastname: Bergamaschi
authors.forename: Francesco
authors.initials: F
authors.lastname: Mazzotta
authors.forename: Masaaki
authors.initials: M
authors.lastname: Miyazawa
authors.forename: Mario
authors.initials: M
authors.lastname: Clerici
mesh_terms: D000071479:APOBEC-1 Deaminase; D016022:Case-Control Studies; D003564:Cytidine Deaminase / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015658:HIV Infections / Q000097:blood / Q000821:virology*; D006679:HIV Seropositivity; D015497:HIV-1 / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D017393:RNA Editing; D012333:RNA, Messenger / Q000032:analysis; D014779:Virus Replication / Q000502:physiology
chemical_list: D012333:RNA, Messenger; D000071479:APOBEC-1 Deaminase; C000605089:APOBEC1 protein, human; D003564:Cytidine Deaminase