[The of gamma-radiation for induction of apomixis in sea-buckthorn (Hippophaë rhamnoides L.)].
The results of studying of the induced apomixis in pollen of sea-buckthorn irradiated by the 60Co gamma-Radiation are considered. Was established that the most effective dose for pollination of the experimental plants is 50 k Gy. In total, from 46 seedlings 19 cases of apomictic origin were revealed, 7 individuals were found to be haploid (n = = 12) and 19 ones were diploid (2n = 24) of maternal origin. Was supposed that apomictic plants (19 seedlings) have parthenogenetic origin. The reason for such conclusion is that the irradiated anomalous pollen tubes despite not having spermia, are entering embryo sac and stimulate the development of apomictic embryo from non-fertilized female gametes. Apparently, pollen tubes cause the induction of DNA replication in the ovules and the development of parthenogenetic plants. Consequently, the described method can be used for the regulation of parthenogenesis in sea-buckthorn to change natural ratio (1 : 1) of male to female plants in desirable quantity.
Todya
V A
VA
Chankseliani
Z Zh
ZZh
Mchedlishvili
M V
MV
D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006238:Haploidy; D031281:Hippophae / Q000502:physiology* / Q000528:radiation effects*; D011058:Pollen / Q000528:radiation effects; D012100:Reproduction, Asexual / Q000528:radiation effects*; D012639:Seeds / Q000502:physiology / Q000528:radiation effects