title: Comparative in vitro study of the proliferation and growth of human osteoblast-like cells on various biomaterials.
abstract: In vitro studies about the growth behavior of osteoblasts onto biomaterials is a basic knowledge and a screening method for the development and application of scaffolds in vivo. In this in vitro study human osteoblast-like (HOB) cells were cultured on seven different biomaterials used in dental and craniomaxillofacial surgery, respectively. The tested biomaterials were synthetic biodegradable (MacroPore, Ethisorb, PDS, Beriplast P) and nonbiodegradable polymers (Palacos) as well as calcium phosphate cement (BoneSource) and titanium. The cell proliferation and cell colonization were analyzed by scanning electron microscopy and EZ4U-test. Statistical analysis were performed. HOB-like cells cultivated on Ethisorb showed the highest proliferation rate. The proliferation rate was statistically significant compared with Palacos, MacroPore, and BoneSource. Whereas, Beriplast, PDS, and titanium yielded lower proliferation rates. However, there was no statistically significant difference compared with Palacos, MacroPore, and BoneSource. SEM analysis showed no significant difference in individual cell features and cell colonization. But an infiltration and a growth of HOB-like cells throughout the porous structure of Ethisorb, which is formed by crossing fibers, is a striking different feature (macrotopography). This feature can explain the highest proliferation rate of Ethisorb. The results showed that HOB-like cells appear to be sensitive to substrate composition and topography. Moreover, the basis for further studies with such biomaterial/osteoblast constructs in vivo are provided. Further focusing points are developing techniques to fabricate three-dimensional porous biomaterial/cell constructs, studying the tissue reaction and the bone regeneration of such constructs compared with the use of autologous bone.
authors.affiliation: Klinik für Mund-, Kiefer- und Gesichtschirurgie, Universität Freiburg, Hugstetterstrasse 55, D-79106 Freiburg, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Itthichaisri
authors.forename: M
authors.initials: M
authors.lastname: Wiedmann-Al-Ahmad
authors.forename: U
authors.initials: U
authors.lastname: Huebner
authors.forename: A
authors.initials: A
authors.lastname: Al-Ahmad
authors.forename: R
authors.initials: R
authors.lastname: Schoen
authors.forename: R
authors.initials: R
authors.lastname: Schmelzeisen
authors.forename: N-C
authors.initials: NC
authors.lastname: Gellrich
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001672:Biocompatible Materials; D018786:Bone Substitutes; D049109:Cell Proliferation; D024042:Collagen Type I / Q000378:metabolism; D003764:Dental Materials; D015718:Fibrin Tissue Adhesive; D006801:Humans; D006882:Hydroxyapatites; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D015675:Osteocalcin / Q000378:metabolism; D016687:Polydioxanone; D019904:Polymethyl Methacrylate; D023822:Tissue Engineering; D014025:Titanium
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D024042:Collagen Type I; D003764:Dental Materials; D015718:Fibrin Tissue Adhesive; D006882:Hydroxyapatites; C475044:hydroxyapatite cement; D015675:Osteocalcin; D016687:Polydioxanone; D019904:Polymethyl Methacrylate; D014025:Titanium; D000469:Alkaline Phosphatase