Proliferative effect of calcitonin gene-related peptide is induced by at least five proteins as identified by proteome profiling.
Calcitonin gene-related peptide is a 37 amino acid neuropeptide. Although calcitonin gene-related peptide activates a G-protein-coupled receptor, recent evidence suggests that calcitonin gene-related peptide induces more complex signaling cascades including the activation of MAP kinases [Eur J Pharmacol; 389:125-130 (2000), Neuropeptides; 34:229-233 (2000)]. However, the molecular mechanisms of this activation still remain to be elucidated. For the first time we applied a proteomics approach in order to identify molecular targets of calcitonin gene-related peptide downstream signaling in the neuroblastoma cell line SK-N-MC and identified proteins that changed either their expression, location, or their post-translational modifications in a time-dependent manner after calcitonin gene-related peptide stimulation.
Pluder
Franka
F
Institute of Biochemistry, Faculty of Bioscience, Pharmacy and Psychology, University of Leipzig, Brüderstrasse 34, D-04103 Leipzig, Germany.
Sinz
Andrea
A
Beck-Sickinger
Annette G
AG
D000595:Amino Acid Sequence; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology*; D045744:Cell Line, Tumor / Q000166:cytology / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D020869:Gene Expression Profiling; D006801:Humans; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009447:Neuroblastoma / Q000378:metabolism / Q000473:pathology; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism; D011499:Protein Processing, Post-Translational / Q000187:drug effects* / Q000502:physiology; D040901:Proteomics; D018015:Receptors, Calcitonin Gene-Related Peptide / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects* / Q000502:physiology; D013997:Time Factors
D009479:Neuropeptides; D018015:Receptors, Calcitonin Gene-Related Peptide; D020928:Mitogen-Activated Protein Kinases; D015740:Calcitonin Gene-Related Peptide