Interactions between apelin and angiotensin II on rat portal vein.
Apelin (AP), an endogenous ligand of the APJ receptor, is involved in the regulation of cardiovascular homeostasis. Regardless the multiple similarities between AP and angiotensin II (Ang II), their roles seem to be different. We studied both the interactions between Apelin 13 (AP13) and Ang II and to what extent, if any, nitric oxide (NO) is involved. The experiments were performed in endothelium-denuded or endothelium-intact rat portal vein in the presence of 10 microM N(G)-nitro L-arginine methyl ester or 10 microM aminoguanidine. AP13 did not modify the isolated rat portal vein tone by itself, but inhibited the Ang II-induced contractions acting mainly by a NO-dependent mechanism. Our results sustain the hypothesis that AP13 could increase the activity of both constitutive and inducible NO synthase on either endothelium intact or endothelium denuded rat portal vein rings.
Gurzu
Bogdan
B
Faculty of Medicine, Department of Physiology, University of Medicine and Pharmacy Gr. T. Popa, 16 Universitatii Str., RO-700115 Iasi, Romania. bgurzu@yahoo.com
Petrescu
Bogdan Cristian
BC
Costuleanu
Marcel
M
Petrescu
Gheorghe
G
D000804:Angiotensin II / Q000502:physiology*; D000818:Animals; D000073861:Apelin; D002352:Carrier Proteins / Q000502:physiology*; D066298:In Vitro Techniques; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D011169:Portal Vein / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D014666:Vasomotor System / Q000502:physiology*
D000073861:Apelin; C498579:Apln protein, rat; D002352:Carrier Proteins; D036341:Intercellular Signaling Peptides and Proteins; D000804:Angiotensin II