title: The effect of vitamin e on splenocytes apoptosis of gamma- irradiated BALB/c mice.
abstract: Apoptosis, a physiologic mechanism to eliminate unwanted cells, is also induced by ionizing irradiation, through production of free radicals. It has been demonstrated that antioxidants such as vitamin E are able to protect cells from damage caused by free radicals. Taken together we found it reasonable to make an attempt to evaluate the protective effect of vitamin E against apoptosis. The irradiated mice received 1 Gy/day gamma radiation for one day (low dose) or for three successive days (high dose, 3Gy). The splenocytes were then isolated at 6, 14 and 24 h after exposure. DNA gel electrophoresis and DNA fragmentation assay were done in addition to the evaluation of splenocytes cytology.Our results showed that Vitamin E can reduce apoptosis against low dose irradiation. However it is not able to completely block programmed cell death in high dose irradiation.
authors.affiliation: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran. ardestani@ibb.ut.ac.ir.
authors.forename: Bahman
authors.initials: B
authors.lastname: Maroufi
authors.forename: Sussan
authors.initials: S
authors.lastname: Kaboudanian Ardestani
authors.forename: Amina
authors.initials: A
authors.lastname: Kariminia
authors.forename: Hussain
authors.initials: H
authors.lastname: Naderimanesh