title: Non-optimal levels of dietary selenomethionine alter splenocyte response and modify oxidative stress markers in female mice.
abstract: Many studies evaluating the effects of selenium (Se) status on immunity utilize inorganic Se, although selenomethionine (Se-Met) has been suggested to be more bioavailable and less toxic. In the current study, we investigated the effects of dietary Se-Met on immune system function and cellular redox status in C57BL/6N female mice fed with low (0.02 ppm), sufficient (0.2 ppm, control group), or excess Se-Met (2 ppm) in the diet for 50 days. Low Se-Met intake reduced glutathione peroxidase (GPx) activity and glutathione concentration without modifying lipoperoxidation. While low Se-Met intake also reduced the number of B cells in the spleen, it increased mitogen-induced proliferation, IL-4 and IL-12 secretion when compared to the sufficient Se-Met intake group. In comparison to controls, excess Se-Met intake increased splenocyte proliferation and reduced B cell numbers, IL-4, and IL-12 secretion without affecting oxidative stress markers. These data suggest that Se-Met supplementation should be carefully evaluated as it many influence immune function.
authors.affiliation: Sección Externa de Toxicología, Centro de Investigación y de Estudios Avanzados, Av. IPN 2508, San Pedro Zacatenco, México, DF 07360, Mexico. lvega@cinvestav.mx
authors.forename: Libia
authors.initials: L
authors.lastname: Vega
authors.forename: Miriam
authors.initials: M
authors.lastname: Rodríguez-Sosa
authors.forename: Eliud A
authors.initials: EA
authors.lastname: García-Montalvo
authors.forename: Luz María
authors.initials: LM
authors.lastname: Del Razo
authors.forename: Guillermo
authors.initials: G
authors.lastname: Elizondo
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000633:toxicity; D001402:B-Lymphocytes / Q000187:drug effects / Q000473:pathology; D015415:Biomarkers / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D004032:Diet; D004305:Dose-Response Relationship, Drug; D005260:Female; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007107:Immune System / Q000187:drug effects* / Q000473:pathology; D018664:Interleukin-12 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000187:drug effects*; D012645:Selenomethionine / Q000008:administration & dosage* / Q000633:toxicity; D013154:Spleen / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D015430:Weight Gain / Q000187:drug effects
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D018664:Interleukin-12; D015847:Interleukin-4; D012645:Selenomethionine; D005979:Glutathione Peroxidase; D005978:Glutathione