Coagulation parameters in apheresis and leukodepleted whole-blood plasma during storage.
BACKGROUND
Thawing fresh-frozen plasma (FFP) may cause delay in delivery, and one approach to circumvent this is to store plasma at +4 degrees C. Thawed plasma is commonly discarded after a few days of storage, owing to the assumption that coagulation factor activity decreases to clinically unacceptable levels.


METHODS
Eighteen apheresis plasma (AP) units were collected from blood donors. The collected plasma was divided into two equal parts: one part frozen at -74 degrees C as FFP and one part stored at +4 degrees C as fresh liquid plasma (FLP). Thirty-nine units of whole blood (WB) were collected from blood donors and leukodepleted by inline filtration, followed by plasma separation. Twenty plasma units were frozen at -74 degrees C as FFP and 19 plasma units were stored at +4 degrees C as FLP for 28 days. Plasma aliquots were collected before freezing and immediately after thawing FFP and before and during storage of FLP at Days 14 and 28. Factor (F)V, FVIII, D-dimers, and C1-esterase inhibitor levels were assessed.


RESULTS
No significant differences in coagulation factor levels were assessed between FLP prepared from AP and FLP prepared from WB. FV and FVIII levels decreased on average 25 and 50 percent, respectively, at Day 14 of storage. C1-esterase inhibitor and D-dimers levels were not affected.


CONCLUSIONS
Leukodepleted apheresis and WB plasma stored for 14 days retain sufficient levels of FV and FVIII activity for maintenance of normal hemostasis and could therefore be considered useful in selected clinical situations.
Boström
F
F
Department of Clinical Immunology, Karolinska University Hospital, Karolinska Institutet, Stockholm, Sweden. fredrik.bostrom@karolinska.se
Sjödahl
M
M
Wehlin
L
L
Egberg
N
N
Lundahl
J
J
D001777:Blood Coagulation; D001779:Blood Coagulation Factors / Q000032:analysis; D001781:Blood Component Removal / Q000379:methods*; D001793:Blood Preservation / Q000009:adverse effects*; D001800:Blood Specimen Collection / Q000379:methods*; D015925:Cryopreservation; D006801:Humans; D047589:Leukocyte Reduction Procedures; D007962:Leukocytes; D012034:Refrigeration
D001779:Blood Coagulation Factors