Differentiation of mesophilic and thermophilic bacteria with fourier transform infrared spectroscopy.
In the present study the characterization and differentiation of mesophilic and thermophilic bacteria were investigated by using Fourier transform infrared (FT-IR) spectroscopy. Our results showed significant differences between the FT-IR spectra of mesophilic and thermophilic bacteria. The protein-to-lipid ratio was significantly higher for thermophiles compared to mesophiles. The absorption intensity of the CH(3) asymmetric stretching vibration was higher in thermophilic bacteria, indicating a change in the composition of the acyl chains. The higher intensity/area observed in the CH(2) symmetric stretching mode at 2857 cm(-1), and the CH(2) bending vibration band at 1452 cm(-1), indicated a higher amount of saturated lipids in thermophilic bacteria. The lipid C=O stretching vibration at 1739 cm(-1), which was observed in the mesophilic group, was not observed clearly in the thermophilic group, indicating a difference in packing that is presumably due to the decreased proportion of unsaturated acyl chains in thermophilic bacteria. In addition, the carbonyl groups become hydrogen bonded and the cellular DNA content was lower in thermophilic bacteria. Moreover, in the 1000-400 cm(-1) frequency region, the spectra of each bacterial species belonging to both the mesophilic and thermophilic bacterial groups, showed characteristic differences that were discriminated via dendrogram using cluster analysis. The current study implies that FT-IR spectroscopy could be successfully applied for the rapid comparison of bacterial groups and species to establish either similarities or discrepancies, as well as to confirm biochemical or physiological characteristics.
Garip
Sebnem
S
Department of Biology, Middle East Technical University, 06531 Ankara, Turkey.
Bozoglu
Faruk
F
Severcan
Feride
F
D001419:Bacteria / Q000737:chemistry*; D018407:Bacterial Physiological Phenomena; D001426:Bacterial Proteins / Q000737:chemistry; D016000:Cluster Analysis; D003470:Culture Media; D004269:DNA, Bacterial / Q000737:chemistry; D003627:Data Interpretation, Statistical; D007202:Indicators and Reagents; D008055:Lipids / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
D001426:Bacterial Proteins; D003470:Culture Media; D004269:DNA, Bacterial; D007202:Indicators and Reagents; D008055:Lipids