Xenopus Xp54 and human RCK/p54 helicases functionally replace yeast Dhh1p in brome mosaic virus RNA replication.
By using a Brome mosaic virus (BMV)-Saccharomyces cerevisiae system, we previously showed that the cellular Lsm1p-7p/Pat1p/Dhh1p decapping-activator complex functions in BMV RNA translation and replication. As a first approach in investigating whether the corresponding human homologues play a similar role, we expressed human Lsm1p (hLsm1p) and RCK/p54 in yeast. Expression of RCK/p54 but not hLsm1p restored the defect in BMV RNA translation and replication observed in the dhh1Delta and lsm1Delta strains, respectively. This functional conservation, together with the common replication strategies of positive-stranded RNA viruses, suggests that RCK/p54 may also play a role in the replication of positive-stranded RNA viruses that infect humans.
Alves-Rodrigues
Isabel
I
Departamento Ciencias Experimentales y de la Salud, Universitat Pompeu Fabra, Dr. Aiguader 80, 08003 Barcelona, Spain.
Mas
Antonio
A
Díez
Juana
J
D000818:Animals; D017795:Bromovirus / Q000502:physiology*; D031724:Bromus; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000502:physiology*; D017353:Gene Deletion; D005816:Genetic Complementation Test; D006801:Humans; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000502:physiology*; D020365:RNA Helicases / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D014779:Virus Replication; D014981:Xenopus; D029867:Xenopus Proteins / Q000235:genetics / Q000502:physiology*
D011518:Proto-Oncogene Proteins; D029701:Saccharomyces cerevisiae Proteins; D029867:Xenopus Proteins; C094683:DDX6 protein, human; C486437:Xp54 RNA helicase, Xenopus; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases