title: Tracing field hybridization in Ryegrass species using microsatellite and morphological markers.
abstract: We have assessed the utility of morphological and microsatellite markers for tracing field hybridization between Lolium multiflorum and Lolium perenne in cereal-enclosed gene flow plots. The presence of awns on the inflorescence of F(1) hybrids was found to be a reliable, but underscoring, indicator of L. multiflorum paternity in L. perenne derived seed as determined by inheritance of species-specific alleles at the microsatellite locus 'H01 H06' in these progeny. A positive correlation was evident in the experimental treatment between the number of pollen donor plants in a given plot and the frequency of hybrid F(1) seed harvested from pollen receptor plants in that plot. These experiments have established the utility of naturally occurring heritable markers for the measurement of gene flow rates in field Ryegrass populations, with particular significance for risk assessment modeling of potential gene flow from transgenic grass cultivars.
authors.affiliation: Molecular Ecology Laboratory, Institute of Bioengineering and Agroecology, National University of Ireland, Maynooth, Co. Kildare, Ireland.
authors.forename: Eimear
authors.initials: E
authors.lastname: Ryan
authors.forename: Ewen
authors.initials: E
authors.lastname: Mullins
authors.forename: James
authors.initials: J
authors.lastname: Burke
authors.forename: Martin
authors.initials: M
authors.lastname: Downes
authors.forename: Conor
authors.initials: C
authors.lastname: Meade
mesh_terms: D000483:Alleles; D051456:Gene Flow; D005819:Genetic Markers; D006824:Hybridization, Genetic; D008129:Lolium / Q000033:anatomy & histology / Q000235:genetics* / Q000502:physiology; D018895:Microsatellite Repeats; D011058:Pollen / Q000235:genetics; D016133:Polymerase Chain Reaction; D013045:Species Specificity
chemical_list: D005819:Genetic Markers