Determination of xanthohumol in hops (Humulus lupulus L.) by nonaqueous CE.
Xanthohumol (XN) is a prenylated chalcone with antimutagenic and anticancer activity from hops. A nonaqueous reverse polarity capillary electrophoretic method for the determination of XN in hop extract was developed and validated. The optimal parameters were a 64.5 cm long fused-silica capillary with 50 microm id at 25 degrees C; 30 kV negative voltage (anode at detector side of the capillary); nonaqueous buffer with 75 mM NaOH and 50 mM boric acid in methanol; hydrodynamical injection with 10 mbar for 40 s; and detection at 440 nm. XN, isoxanthohumol (IX), colupulone, adlupulone, and n-lupulone were well resolved on the electropherogram. The LOD for XN was 0.05 mg/L and RSD for peak area was below 3%. The amount of XN in different samples of hop pellets varied from 0.14 to 0.42%.
Kac
Javor
J
Faculty of Pharmacy, University of Ljubljana, Ljubljana, Slovenia. javor.kac@ffa.uni-lj.si
Zakrajsek
Jure
J
Mlinaric
Ales
A
Kreft
Samo
S
Filipic
Metka
M
D001888:Boric Acids / Q000737:chemistry; D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D005419:Flavonoids; D027582:Humulus / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry; D011427:Propiophenones / Q000032:analysis*; D012822:Silicon Dioxide / Q000737:chemistry; D012972:Sodium Hydroxide / Q000737:chemistry
D001888:Boric Acids; D002021:Buffers; D005419:Flavonoids; D011427:Propiophenones; D012972:Sodium Hydroxide; D012822:Silicon Dioxide; C032688:boric acid; C104536:xanthohumol; D000432:Methanol