Genomic sequence and expression profile of murine Bat1a and Nfkbil1.
In humans, susceptibility to several immunopathologic diseases maps to a conserved block encompassing the polymorphic BAT1, NFKBIL1 (IKBL) and TNF genes in the central MHC. As a pre-requisite for studies of these genes in animal models, we characterized Bat1a and Nfkbil1 in inbred mice differing in their H2 haplotype. We identified two indels and nine single nucleotide polymorphisms (SNP) upstream of Nfkbil1, one indel, nine SNP upstream of Bat1a and a synonymous SNP in exon 2 of Bat1a. H2(g7) and H2(b) mice yielded identical Bat1a and Nfkbil1 sequences. Real time PCR (RT-PCR) showed Bat1a was expressed in adult brain, heart, kidney, liver, lung, pancreas and spleen. Expression of Bat1a was higher in brain and liver of 15-day embryos compared to 1-day old mice and increased moderately in liver and lung of adult mice 2-4 h after LPS challenge. Nfkbil1 expression was low or undetetectable in all tissues and cell lines.
Allcock
Richard
R
School of Surgery and Pathology, University of Western Australia, Nedlands 6009, WA, Australia.
Dolecki
Katarzyna J
KJ
Boodhoo
Alvin
A
Abdul Khalil
Alizan
A
Wong
Agnes M L
AM
Price
Patricia
P
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D001483:Base Sequence; D053487:DEAD-box RNA Helicases / Q000235:genetics* / Q000378:metabolism; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D008070:Lipopolysaccharides; D008285:Major Histocompatibility Complex / Q000235:genetics*; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D012316:RNA Nucleotidyltransferases / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; D017931:DNA Primers; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D008070:Lipopolysaccharides; C518886:Nfkbil1 protein, mouse; D009687:Nuclear Proteins; D014157:Transcription Factors; C518885:Ddx39b protein, mouse; D012316:RNA Nucleotidyltransferases; D053487:DEAD-box RNA Helicases