[Anaerobic humus respiration by Shewanella cinica D14T].
Experimental results suggested Shewanella cinica D14T is capable of humus respiration utilizing various organic acids and some important environmental pollutants (e.g., toluene. etc) as electron donors and AQS or AQDS as a sole terminal electron acceptor under anaerobic condition. The dissimilatory reduction of 1mmol/L AQDS can couple to the production of enough ATP to support cell growth about 60 generations; The oxidization of electron donors was coupled to the reduction of humus, as reduced humus increased corresponding with increasing of electron donor; The typical inhibitors such as Cu2+ which inhibited Fe-S center, Stigmatellin which was methyl-naphthoquinone model, Dicumarol which inhibited oxidized methyl-naphthoquinone transform to reduced one, Metyrapone which was specific inhibitor for P450 enzyme blocked the humus respiration seriously. These were powerful evidences for humus-respiration by D14.
Xu
Zhi-cheng
ZC
Rubber Research Insititute, CATAS, Danzhou 571737, China.
Hong
Yi-guo
YG
Luo
Wei
W
Chen
Xing-juan
XJ
Sun
Guo-ping
GP
Xu
Mei-ying
MY
Guo
Jun
J
Cen
Ying-hua
YH
D000693:Anaerobiosis; D000880:Anthraquinones / Q000378:metabolism; D001673:Biodegradation, Environmental; D003300:Copper / Q000494:pharmacology; D001728:Dicumarol / Q000494:pharmacology; D004579:Electron Transport; D006812:Humic Substances; D008797:Metyrapone / Q000494:pharmacology; D010084:Oxidation-Reduction; D011090:Polyenes / Q000494:pharmacology; D020592:Shewanella / Q000254:growth & development / Q000378:metabolism*
D000880:Anthraquinones; D006812:Humic Substances; D011090:Polyenes; C408124:anthraquinone-2,6-disulfonate; D003300:Copper; D001728:Dicumarol; C041573:stigmatellin; D008797:Metyrapone