title: Escherichia coli AspP activity is enhanced by macromolecular crowding and by both glucose-1,6-bisphosphate and nucleotide-sugars.
abstract: Escherichia coli ADP-sugar pyrophosphatase (AspP) is a "Nudix" hydrolase that catalyzes the hydrolytic breakdown of ADP-glucose linked to glycogen biosynthesis. Moderate increases of AspP activity in the cell are accompanied by significant reductions of the glycogen content. In vitro analyses showed that AspP activity is strongly enhanced by macromolecular crowding and by both glucose-1,6-bisphosphate and nucleotide-sugars, providing a first set of indicative evidences that AspP is a highly regulated enzyme. To our knowledge, AspP is the sole bacterial enzyme described to date which is activated by both G1,6P(2) and nucleotide-sugars.
authors.affiliation: Instituto de Agrobiotecnología, Universidad Pública de Navarra/Gobierno de Navarra/Consejo Superior de Investigaciones Científicas, Carretera de Mutilva s/n, 31192 Mutilva Baja, Navarra, Spain.
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Morán-Zorzano
authors.forename: Alejandro Miguel
authors.initials: AM
authors.lastname: Viale
authors.forename: Francisco José
authors.initials: FJ
authors.lastname: Muñoz
authors.forename: Nora
authors.initials: N
authors.lastname: Alonso-Casajús
authors.forename: Gustavo Gabriel
authors.initials: GG
authors.lastname: Eydallín
authors.forename: Beatriz
authors.initials: B
authors.lastname: Zugasti
authors.forename: Edurne
authors.initials: E
authors.lastname: Baroja-Fernández
authors.forename: Javier
authors.initials: J
authors.lastname: Pozueta-Romero
mesh_terms: D000247:Adenosine Diphosphate Sugars / Q000378:metabolism / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D019298:Glucose-6-Phosphate / Q000031:analogs & derivatives* / Q000494:pharmacology; D006003:Glycogen / Q000378:metabolism; D007700:Kinetics; D046911:Macromolecular Substances; D009702:Nucleoside Diphosphate Sugars / Q000494:pharmacology*; D011755:Pyrophosphatases / Q000378:metabolism*
chemical_list: D000247:Adenosine Diphosphate Sugars; D046911:Macromolecular Substances; D009702:Nucleoside Diphosphate Sugars; D019298:Glucose-6-Phosphate; D006003:Glycogen; C016473:glucose-1,6-bisphosphate; D011755:Pyrophosphatases