title: Long-lasting protective immune response to the 19-kilodalton carboxy-terminal fragment of Plasmodium yoelii merozoite surface protein 1 in mice.
abstract: Merozoite surface protein 1 (MSP1) is the major protein on the surface of the plasmodial merozoite, and its carboxy terminus, the 19-kDa fragment (MSP1(19)), is highly conserved and effective in induction of a protective immune response against malaria parasite infection in mice and monkeys. However, the duration of the immune response has not been elucidated. As such, we immunized BALB/c mice with a standard four-dose injection of recombinant Plasmodium yoelii MSP1(19) formulated with Montanide ISA51 and CpG oligodeoxynucleotide (ODN) and monitored the MSP1(19)-specific antibody levels for up to 12 months. The antibody titers persisted constantly over the period of time without significant waning, in contrast to the antibody levels induced by immunization with Freund's adjuvant, where the antibody levels gradually declined to significantly lower levels 12 months after immunization. Investigation of immunoglobulin G (IgG) subclass longevity revealed that only the IgG1 antibody level (Th2 type-driven response) decreased significantly by 6 months, while the IgG2a antibody level (Th1 type-driven response) did not change over the 12 months after immunization, but the boosting effect was seen in the IgG1 antibody responses but not in the IgG2a antibody responses. After challenge infection, all immunized mice survived with negligibly patent parasitemia. These findings suggest that protective immune responses to MSP1(19) following immunization using oil-based Montanide ISA51 and CpG ODN as an adjuvant are very long-lasting and encourage clinical trials for malaria vaccine development.
authors.affiliation: Department of Microbiology, Mahidol University, Bangkok, Thailand.
authors.forename: Pimmada
authors.initials: P
authors.lastname: Jeamwattanalert
authors.forename: Yuvadee
authors.initials: Y
authors.lastname: Mahakunkijcharoen
authors.forename: Leera
authors.initials: L
authors.lastname: Kittigul
authors.forename: Pakpimol
authors.initials: P
authors.lastname: Mahannop
authors.forename: Sathit
authors.initials: S
authors.lastname: Pichyangkul
authors.forename: Chakrit
authors.initials: C
authors.lastname: Hirunpetcharat
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis / Q000097:blood; D018899:CpG Islands / Q000276:immunology; D005260:Female; D008288:Malaria / Q000276:immunology / Q000517:prevention & control*; D017780:Malaria Vaccines / Q000008:administration & dosage* / Q000276:immunology; D008353:Mannitol / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000276:immunology; D020066:Merozoite Surface Protein 1 / Q000008:administration & dosage / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009829:Oleic Acids / Q000008:administration & dosage / Q000276:immunology; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage; D010446:Peptide Fragments / Q000008:administration & dosage / Q000276:immunology; D010967:Plasmodium yoelii / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000008:administration & dosage
chemical_list: D000276:Adjuvants, Immunologic; D000913:Antibodies, Protozoan; C408982:CPG-oligonucleotide; D017780:Malaria Vaccines; D020066:Merozoite Surface Protein 1; D009829:Oleic Acids; D009838:Oligodeoxyribonucleotides; D010446:Peptide Fragments; D014614:Vaccines, Synthetic; C477385:montanide ISA 51; D008353:Mannitol