Crohn's disease and polymorphism of heat shock protein gene HSP70-2 in the Tunisian population.
OBJECTIVE
Crohn's disease is a multifactorial disorder with a pivotal role of the genetic component. HSP70-2 gene, located in IBD3 region, has a PstI polymorphic site associated recently with Crohn's disease especially with a perforating form. In this study, we sought to determine whether this polymorphism was associated with Crohn's disease in the Tunisian population and its correlation with clinical manifestation of the disease.


METHODS
In all, 148 patients with Crohn's disease and 81 healthy individuals were genotyped for the HSP70-2 PstI polymorphism by restriction fragment length polymorphism analysis.


RESULTS
The allele and genotype frequency of the PstI polymorphism did not differ between patients and controls. Furthermore, this polymorphism was not associated with specific disease behavior.


CONCLUSIONS
This study reported the absence of association between Crohn's disease and HSP70-2 gene in the Tunisian population. The allele A of PstI polymorphism was not associated with phenotype of the disease.
Zouiten-Mekki
Lilia
L
Department of Gastroenterology, La Rabta Hospital, Tunis, Tunisia. w.mekki@laposte.net
Karoui
Sami
S
Kharrat
Maher
M
Fekih
Monia
M
Matri
Samira
S
Boubaker
Jalel
J
Filali
Azza
A
Chaabouni
Habiba
H
D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D003424:Crohn Disease / Q000235:genetics* / Q000601:surgery; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D008297:Male; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D013997:Time Factors
D018840:HSP70 Heat-Shock Proteins; C091879:HSPA2 protein, human