Depolarization and cardiolipin depletion in aged rat brain mitochondria: relationship with oxidative stress and electron transport chain activity.
A noticeable loss of cardiolipin, a significant accumulation of fluorescent products of lipid peroxidation and an increased ability to produce reactive oxygen species in vitro are characteristics of aged rat brain mitochondria, as has been demonstrated in this study. In contrast mitochondrial electron transport chain activity is not significantly compromised except a marginal decline in complex IV activity in aged rat brain. On the other hand, a striking loss of mitochondrial membrane potential occurs in brain mitochondria during aging, which may be attributed to peroxidative membrane damage in this condition. Such mitochondrial dysfunctions as reported here may lead to uncoupling of oxidative phosphorylation, ATP depletion and activation of apoptotic cascade in aged rat brain.
Sen
Tanusree
T
Department of Biochemistry, Dr. B.C. Roy Post-graduate Institute of Basic Medical Sciences & IPGMER, 244B, Acharya J.C. Bose Road, Calcutta 700020, India.
Sen
Nilkantha
N
Jana
Sirsendu
S
Khan
Firoj Hossain
FH
Chatterjee
Uttara
U
Chakrabarti
Sasanka
S
D000375:Aging / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D002308:Cardiolipins / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000378:metabolism*; D005260:Female; D008297:Male; D008564:Membrane Potentials; D008928:Mitochondria / Q000378:metabolism*; D018384:Oxidative Stress; D051381:Rats
D002308:Cardiolipins; D003576:Electron Transport Complex IV