title: Role of the CD8+ dendritic cell subset in transmission of prions.
abstract: Controversial results have been observed in mouse models regarding the role of lymphoid tissues in prion pathogenesis. To investigate the role of dendritic cells (DC), we used a transgenic mouse model. In this model (CD11c-N17Rac1), a significant reduction of CD8+ CD11c(hi) DC has been described, and the remaining CD8+ DC demonstrate a reduced capacity for the uptake of apoptotic cells. After intraperitoneal prion infection, significantly longer incubation times were observed in CD11c-N17Rac1 mice than in controls, indicating that a defect in CD8+ CD11c(hi) DC significantly impedes neuroinvasion after intraperitoneal infection. In contrast, no distinct differences were observed between CD11c-N17Rac1 mice and controls after oral infection. This provides evidence that oral and intraperitoneal prion infections differ in lymphoreticular requirements.
authors.affiliation: Center for Neuropathology and Prion Research, Ludwig Maximilians University, Feodor-Lynen-Strasse 23, 81377 Munich, Germany.
authors.forename: Shneh
authors.initials: S
authors.lastname: Sethi
authors.forename: Kristen M
authors.initials: KM
authors.lastname: Kerksiek
authors.forename: Thomas
authors.initials: T
authors.lastname: Brocker
authors.forename: Hans
authors.initials: H
authors.lastname: Kretzschmar
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001921:Brain / Q000378:metabolism*; D039521:CD11c Antigen / Q000378:metabolism; D016827:CD8 Antigens / Q000378:metabolism; D020566:Dendritic Cells, Follicular / Q000378:metabolism*; D007150:Immunohistochemistry; D007274:Injections, Intraperitoneal; D051379:Mice; D008822:Mice, Transgenic; D017096:Prion Diseases / Q000378:metabolism / Q000635:transmission*; D011328:Prions / Q000008:administration & dosage / Q000378:metabolism*
chemical_list: D039521:CD11c Antigen; D016827:CD8 Antigens; D011328:Prions