title: Species-specific testicular and hepatic microsomal metabolism of benzo(a)pyrene, an ubiquitous toxicant and endocrine disruptor.
abstract: Information on the metabolism of the environmental toxicant, benzo(a)pyrene (BaP) in the male reproductive system is crucial for understanding BaP-induced infertility. Microsomes were isolated from the liver and testes of rat, mouse, hamster, ram, boar, bull, and monkey and incubated with BaP. Post-incubation, samples were extracted with ethyl acetate and analyzed for BaP/metabolites by reverse-phase HPLC with fluorescence detection. A great variation among species to metabolize BaP was observed. The rodent testicular microsomes produced higher proportions of BaP 4,5-diol and 9,10-diol than did boar, ram, bull, and monkey. On the other hand, hepatic microsomes from higher mammals converted a greater proportion of BaP to 3-hydroxy and 9-hydroxy BaP, the detoxification products of BaP. Given the ability of BaP 7-8-diol 9, 10-epoxide, 3-, and 9-hydroxy BaP to bind with DNA and form adducts, there is a likelihood of risk arising from the accumulation of BaP metabolites in testicular tissues. These metabolites may interfere with the formation and function of gametes, eventually contributing to infertility.
authors.affiliation: Department of Biology, Fisk University, Nashville, TN 37208, USA.
authors.forename: Tené L
authors.initials: TL
authors.lastname: Smith
authors.forename: Shayla T
authors.initials: ST
authors.lastname: Merry
authors.forename: Deacqunita L
authors.initials: DL
authors.lastname: Harris
authors.forename: J
authors.initials: J
authors.lastname: Joe Ford
authors.forename: Justus
authors.initials: J
authors.lastname: Ike
authors.forename: Anthony E
authors.initials: AE
authors.lastname: Archibong
authors.forename: Aramandla
authors.initials: A
authors.lastname: Ramesh
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000378:metabolism*; D002273:Carcinogens / Q000378:metabolism*; D002417:Cattle; D006224:Cricetinae; D052244:Endocrine Disruptors / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D008647:Mesocricetus; D051379:Mice; D008810:Mice, Inbred C57BL; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism*; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D009928:Organ Specificity; D051381:Rats; D011916:Rats, Inbred F344; D012756:Sheep; D013045:Species Specificity; D013552:Swine; D013737:Testis / Q000187:drug effects / Q000378:metabolism*
chemical_list: D002273:Carcinogens; D052244:Endocrine Disruptors; D001564:Benzo(a)pyrene