Construction of nanogold hollow balls with dendritic surface as immobilized affinity support for protein adsorption.
This contribution introduced the construction of nanosize gold hollow balls (NGB) with dendritic surface as the immobilized affinity support for aflatoxin B(1) antibody (anti-AFB(1)) adsorption, as a model protein, in biorecognition interface. The interaction between nanogold hollow balls and anti-AFB(1) was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, and quartz crystal microbalance (QCM) technique. The deposited mass of anti-AFB(1) on the nanogold hollow ball-modified electrodes was more than that on the nanogold particle-modified electrodes via QCM analysis. The cyclic voltammogram tends to be more irreversible with anti-AFB(1) concentration increased. The antigen-antibody interaction was examined by using the nanogold hollow ball-modified QCM probes. Experimental results show that the developed protein assay system is sensitive to the concentration of AFB(1) as low as 0.05 ng mL(-1). Thus, the nanogold hollow ball is a useful matrix, and can be used as the immobilized affinity support for the adsorption of other proteins.
Liao
Jia Yao
JY
College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China. liaoswu@msn.com
D000327:Adsorption; D016604:Aflatoxin B1 / Q000276:immunology; D000465:Algorithms; D000908:Antibodies, Fungal / Q000737:chemistry; D050091:Dendrimers; D004563:Electrochemistry; D006046:Gold / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D013499:Surface Properties
D000908:Antibodies, Fungal; D050091:Dendrimers; D011506:Proteins; D006046:Gold; D016604:Aflatoxin B1