A method for Pmo25-associated kinase assay in fission yeast: the activity is dependent on two gC kinases Nak1 and Sid1.
In fission yeast, the conserved proteins, MO25/Pmo25, GC kinase/Nak1, Furry/Mor2, NDR kinase/Orb6, and Mob2, constitute the morphogenesis Orb6 network (MOR). Previously we showed that Pmo25 functions as an upstream component of MOR and that it plays a connecting role between the septation initiation network (SIN) and MOR. Here we establish a Pmo25-associated kinase assay and show that the activity is dependent on Nak1/MOR and Sid1/SIN.
Kume
Kazunori
K
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Japan.
Goshima
Tetsuya
T
Miyahara
Kohji
K
Toda
Takashi
T
Hirata
Dai
D
D002352:Carrier Proteins / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D049452:Green Fluorescent Proteins / Q000235:genetics; D004676:Myelin Basic Protein / Q000235:genetics; D011494:Protein Kinases / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases; D012568:Schizosaccharomyces / Q000201:enzymology*; D029702:Schizosaccharomyces pombe Proteins / Q000032:analysis* / Q000378:metabolism*; D014461:Ultracentrifugation
D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D004676:Myelin Basic Protein; C508251:Pmo25 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins; D049452:Green Fluorescent Proteins; D011494:Protein Kinases; C409036:Sid1 kinase; C500976:Nak1 protein, S pombe; D017346:Protein Serine-Threonine Kinases