High throughput and rapid screening of marine protein hydrolysates enriched in peptides with angiotensin-I-converting enzyme inhibitory activity by capillary electrophoresis.
Twelve kinds of marine protein materials, including fish, shrimp, seashell, algae and seafood wastes were selected for the hydrolysis using four different proteases. The IC(50) values for angiotensin-converting enzyme (ACE) inhibitory activity of 48 hydrolysates were rapidly determined by capillary electrophoresis (CE). The values ranged from 0.17 to 501.7mg/ml, and were affected by both the marine protein resources and the selected proteases. Hydrolysates of the lowest IC(50) values were from shrimp (Acetes chinensis), shark meat, mackerel bone, Polysiphonia urceolata and Spirulina platensis, indicating these five kinds of marine food proteins contained beneficial materials for the production of ACE inhibitory peptides by proteolysis. The hydrolysates obtained using proteases Protamex and SM98011 had lower IC(50) values, showing these two proteases were superior to others. The CE method achieved the same sensitivity as the high performance liquid chromatography (HPLC) method. However, the CE method was faster and, as a result, more economical. Therefore, CE had potential for rapid screening of marine protein hydrolysates enriched in ACE inhibitory peptides.
He
Hai-Lun
HL
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
Chen
Xiu-Lan
XL
Wu
Hao
H
Sun
Cai-Yun
CY
Zhang
Yu-Zhong
YZ
Zhou
Bai-Cheng
BC
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D000818:Animals; D001842:Bone and Bones / Q000737:chemistry; D000458:Cyanobacteria / Q000737:chemistry; D033364:Decapoda / Q000737:chemistry; D056890:Eukaryota / Q000737:chemistry; D005399:Fishes; D008460:Meat; D010455:Peptides / Q000737:chemistry* / Q000494:pharmacology*; D011492:Protein Hydrolysates / Q000737:chemistry* / Q000494:pharmacology*; D012867:Skin / Q000737:chemistry
D000806:Angiotensin-Converting Enzyme Inhibitors; D010455:Peptides; D011492:Protein Hydrolysates