title: Molecular characterization of an ethylene receptor gene (CcETR1) in coffee trees, its relationship with fruit development and caffeine content.
abstract: To understand the importance of ethylene receptor genes in the quality of coffee berries three full-length cDNAs corresponding to a putative ethylene receptor gene (ETR1) were isolated from Coffea canephora cDNA libraries. They differed by their 3'UTR and contained a main ORF and a 5'UTR short ORF putatively encoding a small polypeptide. The CcETR1 gene, present as a single copy in the C. canephora genome, contained five introns in the coding region and one in its 5'UTR. Alternative splicing can occur in C. canephora and C. pseudozanguebariae, leading to a truncated polypeptide. C. pseudozanguebariae ETR1 transcripts showed various forms of splicing alterations. This gene was equally expressed at all stages of fruit development. A segregation study on an inter-specific progeny showed that ETR1 is related to the fructification time, the caffeine content of the green beans, and seed weight. Arabidopsis transformed etiolated seedlings, which over-expressed CcETR1, displayed highly reduced gravitropism, but the triple response was observed in an ethylene enriched environment. These plants behaved like a low-concentration ethylene-insensitive mutant thus confirming the receptor function of the encoded protein. This gene showed no induction during the climacteric crisis but some linkage with traits related to quality.
authors.affiliation: Instituto Nacional de Investigaciones Agrícolas, Bramón, Rubio, Táchira, Venezuela.
authors.forename: José
authors.initials: J
authors.lastname: Bustamante-Porras
authors.forename: Claudine
authors.initials: C
authors.lastname: Campa
authors.forename: Valérie
authors.initials: V
authors.lastname: Poncet
authors.forename: Michel
authors.initials: M
authors.lastname: Noirot
authors.forename: Thierry
authors.initials: T
authors.lastname: Leroy
authors.forename: Serge
authors.initials: S
authors.lastname: Hamon
authors.forename: Alexandre
authors.initials: A
authors.lastname: de Kochko
mesh_terms: D017398:Alternative Splicing; D017360:Arabidopsis / Q000235:genetics; D002110:Caffeine / Q000032:analysis*; D040503:Coffea / Q000737:chemistry / Q000235:genetics* / Q000254:growth & development; D018076:DNA, Complementary; D005638:Fruit / Q000254:growth & development*; D018628:Gene Dosage; D007438:Introns; D008969:Molecular Sequence Data; D010641:Phenotype; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D011110:Polymorphism, Genetic; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D011956:Receptors, Cell Surface; C083750:ethylene receptors, plant; D002110:Caffeine