title: Fluorimetric and mass spectrometric study of the interaction of beta-cyclodextrin and osthole.
abstract: The inclusion complex of beta-cyclodextrin (beta-CD) and osthole was studied by the electrospray ionization mass spectrometry (ESI-MS) and fluorescence spectrometry. From the mass spectrum, the 1:1 stoichiometric inclusion complex of beta-CD and osthole was observed. The tandem mass spectrum was performed. The fluorescence intensity of osthole increased in the present of beta-CD. According to the 1:1 beta-CD-osthole mode, the dissociation constant (KD) was obtained by ESI-MS and fluorescence spectrometry. The KD of beta-CD-osthole inclusion complex is 6.96 x 10(-3) mol L(-1) obtained by mass spectrometry and that is 8.14 x10 (-3) mol L(-1) obtained by fluorescence spectrometry, which is consistent with each other.
authors.affiliation: College of Chemistry, Jilin University, Changchun, PR China.
authors.forename: Huarong
authors.initials: H
authors.lastname: Zhang
authors.forename: Hanqi
authors.initials: H
authors.lastname: Zhang
authors.forename: Chenling
authors.initials: C
authors.lastname: Qu
authors.forename: Lifei
authors.initials: L
authors.lastname: Bai
authors.forename: Lan
authors.initials: L
authors.lastname: Ding
mesh_terms: D003374:Coumarins / Q000737:chemistry*; D007700:Kinetics; D013050:Spectrometry, Fluorescence; D021241:Spectrometry, Mass, Electrospray Ionization; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D003374:Coumarins; D047392:beta-Cyclodextrins; C031215:betadex; C046627:osthol