title: Protein carbonyls as a biomarker of foetal-neonatal hypoxic stress.
abstract: OBJECTIVE
  Investigation of the effect of hypoxic conditions during labour on the protein oxidative modifications and changes in plasma antioxidative capacity of newborns.
  
  
  METHODS
  Oxidative damage to proteins was determined by high-performance liquid chromatography. Antioxidative status was monitored by Trolox equivalent antioxidant capacity method. In our study, 11 hypoxic and 19 normoxic newborns were involved.
  
  
  RESULTS
  In hypoxic newborns, we have found a significant increase in protein carbonyl levels (3.55+/-0.86 versus 3.24+/-0.69 mol carbonyls/mol proteins, p=0.045) and plasma antioxidant capacity (1.76+/-0.056 versus 1.68+/-0.097 mmol Trolox/L, p=0.004) when compared to normoxic children. Bilirubin levels were unchanged (p=0.87).
  
  
  CONCLUSIONS
  Our results show elevated levels of carbonyls in hypoxic neonates compared to normoxic children. The oxidative damage to proteins is not sufficiently prevented by increased antioxidant capacity detected in plasma of hypoxic newborns.
authors.affiliation: Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Sasinkova 2, Bratislava, Slovak Republic.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Zitnanová
authors.forename: Katarína
authors.initials: K
authors.lastname: Sumegová
authors.forename: Martin
authors.initials: M
authors.lastname: Simko
authors.forename: Alexandra
authors.initials: A
authors.lastname: Maruniaková
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Chovanová
authors.forename: Mikulás
authors.initials: M
authors.lastname: Chavko
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Duracková
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D001663:Bilirubin / Q000378:metabolism; D015415:Biomarkers / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D005312:Fetal Blood / Q000378:metabolism*; D006801:Humans; D000860:Hypoxia / Q000503:physiopathology; D007231:Infant, Newborn; D010084:Oxidation-Reduction; D050050:Protein Carbonylation; D011506:Proteins / Q000032:analysis* / Q000378:metabolism
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D011506:Proteins; D001663:Bilirubin