Gene silencing of TKTL1 by RNAi inhibits cell proliferation in human hepatoma cells.
We detected a strong upregulation of the mutated transketolase transcript (TKTL1) in human hepatoma cell line HepG2, whereas transketolase (TKT) and transketolase-like-2 (TKTL2) transcripts were not upregulated. We inhibited the expression of TKTL1 by RNAi in HepG2 cells. It was found that total transketolase activity was dramatically downregulated and the proliferation of cancer cells was significantly inhibited in HepG2 cells. These results indicate that TKTL1 gene influences total transketolase activity and cell proliferation in human hepatoma cells, suggesting that TKTL1 gene plays an important role on glycometabolism in tumors and it might become a novel target for tumor gene therapy.
Zhang
Song
S
Department of Otolaryngology, Union Hospital of Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430022, China.
Yang
Ju-Hong
JH
Guo
Chang-Kai
CK
Cai
Peng-Cheng
PC
D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D020868:Gene Silencing; D006801:Humans; D034622:RNA Interference; D014162:Transfection; D014174:Transketolase / Q000235:genetics* / Q000378:metabolism
C505431:TKTL1 protein, human; D014174:Transketolase