[Effects of programmed cell death on human dental follicle cells and changes of programmed cell death under different hydrostatic pressures].
OBJECTIVE
Tooth eruption requires the presence of the dental follicle (DF) around the unerupted tooth. This study is to investigate programmed cell death on human dental follicle cells and changes of programmed cell death under different hydrostatic pressures: 0, 50 and 100 kPa.


METHODS
Human dental follicles from third mandibular molars were surgically removed from adolescents who need for orthodontics treatment after informed content, then trypsinized and cultured. Human dental follicle cells were divided into three groups according to different hydrostatic pressures: 0, 50 and 100 kPa and their programmed cell death were labeled by using TdT-medi-ated-dUTP nick and labeling (TUNEL).


RESULTS
Dental follicle cells cultured were elongate shape and exhibited fibroblastic characteristics. Compared with 0 kPa, programmed cell death cells on human dental follicle cells were increased 0.23% and 31.65% under 50 kPa and 100 kPa hydrostatic pressures respectively. 100 kPa group increased significantly (P < 0.01).


CONCLUSIONS
It suggested that programmed cell death occured in human dental follicle cells cultured in vitro and was influenced by different hydrostatic pressures. Hydrostatic pressure may improve tooth erup-tion through dental follicle.
Jin
Zuo-Lin
ZL
Dept. of Orthodontics, Stomatological College, The Fourth Military Medical University, Xi'an 710032, China.
Luo
Song-Jiao
SJ
Lin
Zhu
Z
Jiao
Guang-Hai
GH
Wang
Hai-Xue
HX
D000293:Adolescent; D017209:Apoptosis; D003795:Dental Sac; D005347:Fibroblasts; D006801:Humans; D006874:Hydrostatic Pressure; D008963:Molar; D014078:Tooth Eruption