title: Construction of a pair of practical Nocardia-Escherichia coli shuttle vectors.
abstract: We constructed a pair of Nocardia-Escherichia coli shuttle vectors, pNV18 and pNV19, by combining the mycobacterial plasmid pAL5000 with the E. coli vector pK18 or pK19. These vectors have a number of useful features, including small size (4.4 kb), a multiple cloning site, and blue/white selection. To our knowledge, pNV18 and pNV19 are the first cloning vectors for practical use in Nocardia spp.
authors.affiliation: Department of Bioactive Molecules, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Chiba
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Hoshino
authors.forename: Keiko
authors.initials: K
authors.lastname: Ishino
authors.forename: Takahisa
authors.initials: T
authors.lastname: Kogure
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Mikami
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Uehara
authors.forename: Jun
authors.initials: J
authors.lastname: Ishikawa
mesh_terms: D003001:Cloning, Molecular; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D007763:Lac Operon; D009161:Mycobacterium / Q000235:genetics; D009615:Nocardia / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D014031:Tobramycin / Q000494:pharmacology; D014169:Transformation, Bacterial / Q000235:genetics
chemical_list: D014031:Tobramycin