Adenosine added on the primer 5' end improved TA cloning efficiency of polymerase chain reaction products.
Peng
Ri-He
RH
Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Center, Shanghai Academy of Agricultural Sciences, Shanghai, People's Republic of China.
Xiong
Ai-Sheng
AS
Liu
Jin-ge
JG
Xu
Fang
F
Bin
Cai
C
Zhu
Hong
H
Yao
Quan-Hong
QH
D000241:Adenosine; D003001:Cloning, Molecular; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics*; D005822:Genetic Vectors / Q000138:chemical synthesis*; D016133:Polymerase Chain Reaction; D019914:Taq Polymerase / Q000378:metabolism
D017931:DNA Primers; D018744:DNA, Plant; D004274:DNA, Recombinant; D019914:Taq Polymerase; D000241:Adenosine