title: Sequence of plasmid pBS228 and reconstruction of the IncP-1alpha phylogeny.
abstract: The antibiotic resistance plasmid pBS228 has been completely sequenced, and revealed to be descended from a plasmid virtually identical to the Birmingham IncP-1alpha plasmid RK2/RP4/RP1. However, it has three additional transposon insertions, one of which is responsible for the extra antibiotic resistances conferred. Loss of kanamycin resistance, which is characteristic of most IncP-1alpha plasmids, is the result of this insertion. A second transposon causes inactivation of the mating pair formation apparatus, rendering the plasmid non-self-transmissible. Comparison with the published data for other IncP-1alpha plasmids gives insight into the recent evolutionary history of this group as well as the acquisition and transmission of one of the first ampicillin resistance transposons discovered.
authors.affiliation: School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
authors.forename: Anthony S
authors.initials: AS
authors.lastname: Haines
authors.forename: Karen
authors.initials: K
authors.lastname: Jones
authors.forename: Sarah M
authors.initials: SM
authors.lastname: Batt
authors.forename: Irina A
authors.initials: IA
authors.lastname: Kosheleva
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Thomas
mesh_terms: D004251:DNA Transposable Elements / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D019143:Evolution, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D010957:Plasmids / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004251:DNA Transposable Elements