title: Shiga toxin-producing Escherichia coli isolated from patients with diarrhoea in Bangladesh.
abstract: The prevalence of Shiga toxin-producing Escherichia coli (STEC) and its characteristics were determined among hospitalized patients with diarrhoea and children with diarrhoea in an urban slum community of Dhaka city using sensitive culture and PCR methods. Stool samples were collected from 410 patients with diarrhoea enrolled in the 2% surveillance system (every 50th patient attending the hospital with diarrhoeal disease is included) at the ICDDR,B hospital and from 160 children of 2-5 years of age with diarrhoea living in an urban slum in Dhaka, between September 2004 and April 2005. Shiga toxin genes (stx) were detected by multiplex PCR in the enrichment broth of nine samples (2.2%) from hospitalized patients and 11 samples (6.9%) from the community patients. STEC was isolated from five stool samples with positive PCR results using a colony patch technique. All five isolates were positive in the Vero cell assay and PCR fragments of stx genes were confirmed by sequencing. Two isolates were positive for the E. coli attaching-and-effacing (eae) gene and four were positive for the enterohaemolysin (hlyEHEC) gene and enterohaemolysin production. The five isolates belonged to five different serotypes:O32:H25, O2:H45, O76:H19, ONT:H25 and ONT:H19. It can be concluded that STEC is not a common pathogen in Bangladesh among hospitalized patients with diarrhoea nor among mild cases of diarrhoea in the community.
authors.affiliation: Laboratory of Food Microbiology, Wageningen University, The Netherlands.|Food and Consumer Product Safety Authority, Zutphen, The Netherlands.|International Centre for Diarrhoeal Disease Research, Bangladesh: Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh.
authors.forename: M Aminul
authors.initials: MA
authors.lastname: Islam
authors.affiliation: Food and Consumer Product Safety Authority, Zutphen, The Netherlands.
authors.forename: A E
authors.initials: AE
authors.lastname: Heuvelink
authors.affiliation: Food and Consumer Product Safety Authority, Zutphen, The Netherlands.
authors.forename: E
authors.initials: E
authors.lastname: de Boer
authors.affiliation: Department of Medical Microbiology, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands.
authors.forename: P D
authors.initials: PD
authors.lastname: Sturm
authors.affiliation: Laboratory of Food Microbiology, Wageningen University, The Netherlands.
authors.forename: R R
authors.initials: RR
authors.lastname: Beumer
authors.affiliation: Laboratory of Food Microbiology, Wageningen University, The Netherlands.
authors.forename: M H
authors.initials: MH
authors.lastname: Zwietering
authors.affiliation: International Centre for Diarrhoeal Disease Research, Bangladesh: Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh.
authors.forename: A S G
authors.initials: ASG
authors.lastname: Faruque
authors.affiliation: International Centre for Diarrhoeal Disease Research, Bangladesh: Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh.
authors.forename: R
authors.initials: R
authors.lastname: Haque
authors.affiliation: International Centre for Diarrhoeal Disease Research, Bangladesh: Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh.
authors.forename: D A
authors.initials: DA
authors.lastname: Sack
authors.affiliation: International Centre for Diarrhoeal Disease Research, Bangladesh: Centre for Health and Population Research, GPO Box-128, Dhaka-1000, Bangladesh.
authors.forename: K A
authors.initials: KA
authors.lastname: Talukder
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics; D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D001459:Bangladesh; D002648:Child; D002675:Child, Preschool; D002522:Chlorocebus aethiops; D004269:DNA, Bacterial / Q000235:genetics; D003967:Diarrhea / Q000382:microbiology*; D004926:Escherichia coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000096:biosynthesis / Q000235:genetics; D005243:Feces / Q000382:microbiology; D005260:Female; D006460:Hemolysin Proteins / Q000096:biosynthesis / Q000235:genetics; D006761:Hospitals; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012703:Serotyping; D022603:Shiga Toxins / Q000096:biosynthesis* / Q000235:genetics; D014505:Urban Population; D014709:Vero Cells
chemical_list: D018829:Adhesins, Bacterial; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; D022603:Shiga Toxins; C066219:enterohemolysin; C073819:eaeA protein, E coli