title: IgE expression pattern in lung: relation to systemic IgE and asthma phenotypes.
abstract: BACKGROUND
  IgE-mediated responses contribute to allergy and asthma. Little is understood regarding the relationship of tissue IgE to systemic IgE, inflammation or clinical outcomes.
  
  
  OBJECTIVE
  To evaluate local IgE expression and cellular inflammation in the proximal and distal lung of normal subjects and subjects with asthma of varying severity and relate those tissue parameters to systemic IgE levels, atopy, lung function, and history of severe exacerbations of asthma.
  
  
  METHODS
  Tissue from more than 90 subjects with eosinophilic (SAeo(+)) and noneosinophilic (SAeo(-)) severe asthma, mild asthma and normal subjects were immunostained for IgE, signal-amplifying isoform of IgE receptor (FcepsilonRIbeta) and markers of mast cells, eosinophils, and lymphocytes. Tissue expression of IgE, FcepsilonRIbeta, cellular inflammation, serum IgE, and atopy were compared. Regression models were used to determine the relationship of local and systemic IgE to lung function and severe exacerbations of asthma.
  
  
  RESULTS
  Mast cell-bound IgE was present along airways but absent in lung parenchyma. Although the groups were similar in systemic/serum IgE and atopy, local/tissue IgE was highest in SAeo(+) and correlated with eosinophils and lymphocytes (r(s) = 0.52, P < .0001; and r(s) = 0.23, P = .03, respectively). Higher local IgE was associated with better lung function, but also with more severe exacerbations of asthma.
  
  
  CONCLUSIONS
  Local IgE appears to be primarily a component of responses within the mucosal immune compartment and is related to cellular inflammation, lung function, and clinical outcomes in asthma.
  
  
  CONCLUSIONS
  Local/airway IgE-related processes rather than systemic markers of atopy may be relevant in determining clinical outcomes in asthma.
authors.affiliation: Division of Pulmonary, Allergy, and Critical Care Medicine, School of Medicine, University of Pittsburgh, UPMC Montefiore, Pittsburgh, PA 15213, USA. balzars@upmc.edu
authors.forename: Silvana
authors.initials: S
authors.lastname: Balzar
authors.forename: Matthew
authors.initials: M
authors.lastname: Strand
authors.forename: Diane
authors.initials: D
authors.lastname: Rhodes
authors.forename: Sally E
authors.initials: SE
authors.lastname: Wenzel
mesh_terms: D000368:Aged; D001249:Asthma / Q000097:blood / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis* / Q000097:blood* / Q000235:genetics; D008168:Lung / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology; D008875:Middle Aged; D010641:Phenotype; D012129:Respiratory Function Tests; D012720:Severity of Illness Index
chemical_list: D007073:Immunoglobulin E