title: Genetic fidelity of in vitro regenerants, encapsulation of shoot tips and high diosgenin content in Dioscorea bulbifera L., a potential alternative source of diosgenin.
abstract: Dioscorea bulbifera L. containing the pharmaceutically important compound, diosgenin, was regenerated in vitro through nodal segments on supplemented Murashige and Skoog medium (MS). Diosgenin was at 12 mg g(-1)dry wt in 12-week-old plantlets raised on MS with various growth hormones. Random amplified polymorphic DNA (RAPD) analysis showed genetic fidelity of regenerants. Encapsulation of shoot tips in 3% (w/v) calcium alginate for storage and germplasm exchange was achieved.
authors.affiliation: Department of Biotechnology, Faculty of Science, Jamia Hamdard, New Delhi, India.
authors.forename: Alka
authors.initials: A
authors.lastname: Narula
authors.forename: Sanjeev
authors.initials: S
authors.lastname: Kumar
authors.forename: P S
authors.initials: PS
authors.lastname: Srivastava
mesh_terms: D000383:Agriculture / Q000379:methods*; D018744:DNA, Plant / Q000235:genetics*; D027742:Dioscorea / Q000235:genetics / Q000502:physiology*; D004144:Diosgenin / Q000378:metabolism*; D018520:Plant Shoots / Q000502:physiology*; D012038:Regeneration / Q000502:physiology*
chemical_list: D018744:DNA, Plant; D004144:Diosgenin