title: Chlamydial Hsp60-2 is iron responsive in Chlamydia trachomatis serovar E-infected human endometrial epithelial cells in vitro.
abstract: Chlamydial 60-kDa heat shock proteins (cHsp60s) are known to play a prominent role in the immunopathogenesis of disease. It is also known that several stress-inducing growth conditions, such as heat, iron deprivation, or exposure to gamma interferon, result in the development of persistent chlamydial forms that often exhibit enhanced expression of cHsp60. We have shown previously that the expression of cHsp60 is greatly enhanced in Chlamydia trachomatis serovar E propagated in an iron-deficient medium. The objective of this work was to determine which single cHsp60 or combination of the three cHsp60 homologs encoded by this organism responds to iron limitation. Using monospecific polyclonal peptide antisera that recognize only cHsp60-1, cHsp60-2, or cHsp60-3, we found that expression of cHsp60-2 is responsive to iron deprivation. Overall, our studies suggest that the expression of cHsp60 homologs differs among the mechanisms currently known to induce persistence.
authors.affiliation: Department of Microbiology, James H. Quillen College of Medicine, East Tennessee State University, P.O. Box 70579, Johnson City, TN 37614-1708, USA.
authors.forename: Richard W
authors.initials: RW
authors.lastname: LaRue
authors.forename: Brian D
authors.initials: BD
authors.lastname: Dill
authors.forename: David K
authors.initials: DK
authors.lastname: Giles
authors.forename: Judy D
authors.initials: JD
authors.lastname: Whittimore
authors.forename: Jane E
authors.initials: JE
authors.lastname: Raulston
mesh_terms: D018834:Chaperonin 60 / Q000235:genetics / Q000378:metabolism*; D002692:Chlamydia trachomatis / Q000145:classification / Q000254:growth & development* / Q000472:pathogenicity; D004717:Endometrium / Q000166:cytology / Q000382:microbiology*; D004847:Epithelial Cells / Q000382:microbiology*; D005260:Female; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D007501:Iron / Q000378:metabolism*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D012703:Serotyping
chemical_list: D018834:Chaperonin 60; D007501:Iron