title: Lysobacter niabensis sp. nov. and Lysobacter niastensis sp. nov., isolated from greenhouse soils in Korea.
abstract: Two bacterial strains, designated GH34-4(T) and GH41-7(T), were isolated from greenhouse soil cultivated with cucumber. The bacteria were strictly aerobic, Gram-negative, rod-shaped and oxidase- and catalase-positive. 16S rRNA gene sequence analysis indicated that these strains belong to the genus Lysobacter within the Gammaproteobacteria. Strain GH34-4(T) showed highest sequence similarity to Lysobacter yangpyeongensis GH19-3(T) (97.5 %) and Lysobacter koreensis Dae16(T) (96.4 %), and strain GH41-7(T) showed highest sequence similarity to Lysobacter antibioticus DSM 2044(T) (97.5 %), Lysobacter enzymogenes DSM 2043(T) (97.5 %) and Lysobacter gummosus ATCC 29489(T) (97.4 %). Levels of DNA-DNA relatedness indicated that strains GH34-4(T) and GH41-7(T) represented species clearly different from L. yangpyeongensis, L. antibioticus, L. enzymogenes and L. gummosus. The major cellular fatty acids of strains GH34-4(T) and GH41-7(T) were iso-C(16 : 0), iso-C(15 : 0) and iso-C(17 : 1)omega9c, and the major isoprenoid quinone was Q-8. The DNA G+C contents of GH34-4(T) and GH41-7(T) were 62.5 and 66.6 mol%, respectively. On the basis of the polyphasic taxonomic data presented, it is evident that each of these strains represents a novel species of the genus Lysobacter, for which the names Lysobacter niabensis sp. nov. (type strain GH34-4(T)=KACC 11587(T)=DSM 18244(T)) and Lysobacter niastensis sp. nov. (type strain GH41-7(T)=KACC 11588(T)=DSM 18481(T)) are proposed.
authors.affiliation: Applied Microbiology Division, National Institute of Agricultural Science and Technology, Rural Development Administration (RDA), Suwon 441-707, Republic of Korea.
authors.forename: Hang-Yeon
authors.initials: HY
authors.lastname: Weon
authors.affiliation: Korean Agricultural Culture Collection (KACC), Microbial Genetics Division, National Institute of Agricultural Biotechnology, RDA, Suwon 441-707, Republic of Korea.
authors.forename: Byung-Yong
authors.initials: BY
authors.lastname: Kim
authors.affiliation: Environment and Ecology Division, National Institute of Agricultural Science and Technology, RDA, Suwon 441-707, Republic of Korea.
authors.forename: Min-Kyeong
authors.initials: MK
authors.lastname: Kim
authors.affiliation: Korean Agricultural Culture Collection (KACC), Microbial Genetics Division, National Institute of Agricultural Biotechnology, RDA, Suwon 441-707, Republic of Korea.
authors.forename: Seung-Hee
authors.initials: SH
authors.lastname: Yoo
authors.affiliation: Korean Agricultural Culture Collection (KACC), Microbial Genetics Division, National Institute of Agricultural Biotechnology, RDA, Suwon 441-707, Republic of Korea.
authors.forename: Soon-Wo
authors.initials: SW
authors.lastname: Kwon
authors.affiliation: Korean Agricultural Culture Collection (KACC), Microbial Genetics Division, National Institute of Agricultural Biotechnology, RDA, Suwon 441-707, Republic of Korea.
authors.forename: Seung-Joo
authors.initials: SJ
authors.lastname: Go
authors.affiliation: Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, D-38124 Braunschweig, Germany.
authors.forename: Erko
authors.initials: E
authors.lastname: Stackebrandt
mesh_terms: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids / Q000032:analysis; D007723:Korea; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D012988:Soil Microbiology; D044166:Xanthomonadaceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S