Essential role of Elovl4 in very long chain fatty acid synthesis, skin permeability barrier function, and neonatal survival.
Mutations in the gene ELOVL4 have been shown to cause stargardt-like macular dystrophy. ELOVL4 is part of a family of fatty acid elongases and is yet to have a specific elongase activity assigned to it. We generated Elovl4 Y270X mutant mice and characterized the homozygous mutant as well as homozygous Elovl4 knockout mice in order to better understand the function or role of Elovl4. We found that mice lacking a functional Elovl4 protein died perinatally. The cause of death appears to be from dehydration due to faulty permeability barrier formation in the skin. Further biochemical analysis revealed a significant reduction in free fatty acids longer than C26 in homozygous mutant and knockout mouse skin. These results implicate the importance of these long chain fatty acids in skin barrier development. Furthermore, we suggest that Elovl4 is likely involved in the elongation of C26 and longer fatty acids.
Cameron
D Joshua
DJ
Department of Ophthalmology and Visual Sciences, Moran Eye Center, University of Utah School of Medicine, Salt Lake City, UT 84132, USA.
Tong
Zongzhong
Z
Yang
Zhenglin
Z
Kaminoh
Jack
J
Kamiyah
Shin
S
Chen
Haoyu
H
Zeng
Jiexi
J
Chen
Yali
Y
Luo
Ling
L
Zhang
Kang
K
D000818:Animals; D000831:Animals, Newborn; D001835:Body Weight; D003681:Dehydration; D005136:Eye Proteins / Q000235:genetics / Q000378:metabolism*; D005227:Fatty Acids / Q000096:biosynthesis* / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D016014:Linear Models; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009154:Mutation; D010539:Permeability; D012867:Skin / Q000196:embryology / Q000378:metabolism / Q000473:pathology; D012879:Skin Physiological Phenomena; D015996:Survival Rate
C498834:Elovl4 protein, mouse; D005136:Eye Proteins; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D008565:Membrane Proteins