Anti-TNF-alpha antibody reduces pain-behavioral changes induced by epidural application of nucleus pulposus in a rat model depending on the timing of administration.
METHODS
An experimental animal study.


OBJECTIVE
To study if antitumor necrosis factor-alpha (TNF-alpha) antibody, which is administered at different times, reduces the pain behavior induced by application of nucleus pulposus (NP) to the nerve root.


BACKGROUND
Treatment with TNF-alpha inhibitor reduces the pain-related behavior induced by epidural application of NP in rats.


METHODS
Left L5 partial laminectomy was performed and NP was applied to the L5 nerve root in 24 rats. The rats were divided into 4 groups. In 3 groups, anti-rat TNF-alpha antibody was intravenously administered immediately after, or 6 or 20 days after NP application. The fourth group was not treated with anti-rat TNF-alpha antibody (untreated rats). The withdrawal threshold of the plantar surface was determined 1 day before up through 28 days after NP application.


RESULTS
The withdrawal threshold of rats that had been treated with anti-rat TNF-alpha antibody immediately after or 6 days after, but not 20 days after, NP application, was significantly higher than that of the untreated rats.


CONCLUSIONS
Anti-TNF-alpha antibody reduced allodynia only when it was administered soon after the onset of allodynia. Late administration of anti-TNF-alpha antibody did not have an antiallodynic effect.
Sasaki
Nobuhisa
N
Department of Orthopedic Surgery, Fukushima Medical University School of Medicine, Fukushima City, Fukushima, Japan. non@fmu.ac.jp
Kikuchi
Shin-ichi
S
Konno
Shin-ichi
S
Sekiguchi
Miho
M
Watanabe
Kazuyuki
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000627:therapeutic use*; D004824:Epidural Space / Q000503:physiopathology; D000069285:Infliximab; D007275:Injections, Intravenous; D007403:Intervertebral Disc / Q000503:physiopathology* / Q000637:transplantation; D007405:Intervertebral Disc Displacement / Q000378:metabolism / Q000503:physiopathology*; D008159:Lumbar Vertebrae / Q000294:innervation / Q000503:physiopathology; D008297:Male; D010146:Pain / Q000188:drug therapy* / Q000209:etiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014182:Transplantation, Autologous; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D014409:Tumor Necrosis Factor-alpha; D000069285:Infliximab