title: Identification of a fibrinolytic enzyme by Bacillus vallismortis and its potential as a bacteriolytic enzyme against Streptococcus mutans.
abstract: A potent fibrinolytic enzyme-producing bacterium was isolated from the traditional Korean condiment Chungkook-jang and identified as Bacillus vallismortis Ace02. The extracellular fibrinolytic enzyme was purified with a 18% recovery of activity from supernatant cultures using CM-Sepharose column chromatography and Sephacryl S-200 gel filtration. The specific activity of the purified enzyme was 757 kFU mg(-1). Its molecular mass was about 28 kDa and the initial amino acids of the N-terminal sequence were AQSVPYGVSQ. The full amino acid sequence of fibrinolytic enzyme Ace02 corresponded with bacteriolytic enzyme, L27, from Bacillus licheniformis, which has strong lytic activity against Streptococcus mutans, a major causative strain of dental caries. This suggests that the purified enzyme should be used for prevention of dental caries as well as being an effective thrombolytic agent.
authors.affiliation: AceBiotech Co., Ltd., #114 Bio-Venture Center, KRIBB, Yuseong, Daejeon, Korea.
authors.forename: Jae-Bum
authors.initials: JB
authors.lastname: Kim
authors.forename: Woo-Hyuk
authors.initials: WH
authors.lastname: Jung
authors.forename: Ji-Myung
authors.initials: JM
authors.lastname: Ryu
authors.forename: Yeo-Joon
authors.initials: YJ
authors.lastname: Lee
authors.forename: Joon-Ki
authors.initials: JK
authors.lastname: Jung
authors.forename: Hyung-Wook
authors.initials: HW
authors.lastname: Jang
authors.forename: Sung-Woo
authors.initials: SW
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology*; D001433:Bacteriolysis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005240:Feasibility Studies; D005342:Fibrinolysis; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000008:administration & dosage* / Q000737:chemistry* / Q000378:metabolism; D013295:Streptococcus mutans / Q000166:cytology* / Q000187:drug effects*
chemical_list: D010447:Peptide Hydrolases