title: Removing the regulatory N-terminal domain of cardiac troponin I diminishes incompatibility during bacterial expression.
abstract: Troponin I (TnI) is a muscle-specific protein and plays an allosteric function in the Ca(2+) regulation of cardiac and skeletal muscle contraction. Expression of cloned cDNA in Escherichia coli is an essential approach to preparing human TnI and mutants for structural and functional studies. The expression level of cardiac TnI in E. coli is very low. To reduce the potential toxicity of cardiac TnI to the host cell, we constructed a bi-cistronic expression vector to co-express cardiac TnI and cardiac/slow troponin C (TnC), a natural binding partner of TnI and a protein that readily expresses in E. coli at high levels. The co-expression moderately increased the expression of cardiac TnI although a high amount of TnC protein was produced from the bi-cistronic mRNA. The use of an E. coli strain containing additional tRNAs for certain low bacterial usage eukaryotic codons improved the expression of cardiac TnI. Modifications of two 5'-regional codons that have predicted low usages in bacterial cells did not reproduce the improvement, indicating that not the 5' but the overall codon usage restricts the translational efficiency of cardiac TnI mRNA in E. coli. However, deletion of the cardiac TnI-specific N-terminal 28 amino acids significantly improved the protein expression independent of the host cell tRNA modifications. The results suggest that the regulatory N-terminal domain of cardiac TnI is a dominant factor for the incompatibility in bacterial cells, supporting its role in modulating the overall molecular conformation.
authors.affiliation: Section of Molecular Cardiology, Evanston Northwestern Healthcare and Northwestern University Feinberg School of Medicine, Evanston, IL 60201, USA.
authors.forename: Zhi-Bin
authors.initials: ZB
authors.lastname: Yu
authors.forename: Jiang-Ping
authors.initials: JP
authors.lastname: Jin
mesh_terms: D000328:Adult; D000818:Animals; D003001:Cloning, Molecular / Q000379:methods; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005822:Genetic Vectors / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D051379:Mice; D009206:Myocardium / Q000378:metabolism*; D010446:Peptide Fragments / Q000096:biosynthesis / Q000235:genetics* / Q000633:toxicity*; D011487:Protein Conformation; D017434:Protein Structure, Tertiary / Q000235:genetics; D017384:Sequence Deletion / Q000235:genetics*; D019209:Troponin C / Q000096:biosynthesis / Q000235:genetics; D019210:Troponin I / Q000096:biosynthesis* / Q000235:genetics* / Q000633:toxicity
chemical_list: D010446:Peptide Fragments; D019209:Troponin C; D019210:Troponin I