[Effect of weiganli on apoptosis and expression of Bcl-2, bax protein in bone marrow nucleated cells of anemic mice].
OBJECTIVE
To study the effect of weiganli on apoptosis and expression of Bcl-2 Bax protein in bone marrow nucleated cells ( BMNCs) of anemic mice and investigate its mechanism, so as to find its new plinic use.


METHODS
Effects of WeiGanLi on the amount of the peripheral blood cells and BMNCs were determined on the model of myelosuppressed anemic mice. The cell cycle of BMNCs was analyzed by flow cytometer. The apoptosis of BMNCs was detected by TUNEL, and the expression of Bcl-2 and Bax protein were detected by immunohistochemical method.


RESULTS
Weiganli could significantly increase the number of RBC, WBC, HGb, Phit as well as the number of BNMCs, significantly active BNMCs from stasis into cell cycling and Markedly decrease the apoptosis of BNMCs after 2.0 Gy 60Co gamma irradition while the expression of Bcl-2 increased and Bax decreased.


CONCLUSIONS
Weiganli can significantly inhibit the apoptosis of BMNCs probably due to its enhancing effects on Bcl-2 expression, and inhibitory effect on Bax expression. In this way, it prevents the blood cells from injury, and contributes to the rehabilitation of the organic blood production.
Chen
Yong-feng
YF
School of Basic Medical Sciences of Sichuan University, ChengDu 610041, China. chenyongfeng@126.com
Zhu
Bi-de
BD
Zhang
Xin-sheng
XS
Lü
Yan
Y
D000740:Anemia / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D005434:Flow Cytometry; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010946:Plants, Medicinal / Q000737:chemistry; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis
D004365:Drugs, Chinese Herbal; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein