[Expression of synaptophysin in experimental diffuse brain injury].
OBJECTIVE
To investigate the changes in the expression_level of synaptophysin following diffuse brain injury (DBI) in rats and to correlate the changes of the synaptophysin expression_level with the post injury time interval.


METHODS
Wister rats were used as a DBI model induced by Marmarou method. The changes of synaptophysin immunoreactivity on coronal sections of the rats sampled at different post-injury time intervals were used as a marker. The densitometry of the synaptophysin immunoreactivity was documented by imaging technique and analyzed by SPSS software.


RESULTS
The expression level of synaptophysin in DBI rats showed dynamic changes following DBI as well as during the repairing period.


CONCLUSIONS
The changes of synaptophysin level may be used as a marker for estimation of the post injury time interval in DBI.
Fan
Shuan-liang
SL
Department of Forensic Science, Medical College, Xi'an Jiaotong University, Xi'an 710061, China. xafansl@mail.xjtu.edu.cn
Huang
Ping
P
Liu
Yan-feng
YF
Zhang
Pin
P
Wang
Zhen-yuan
ZY
D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D001930:Brain Injuries / Q000378:metabolism* / Q000473:pathology; D002540:Cerebral Cortex / Q000378:metabolism / Q000473:pathology; D020833:Diffuse Axonal Injury / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D020198:Intracranial Hemorrhage, Traumatic / Q000378:metabolism / Q000473:pathology; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling; D013569:Synapses / Q000378:metabolism / Q000473:pathology; D016708:Synaptophysin / Q000378:metabolism*; D013997:Time Factors
D016708:Synaptophysin