[Mitigating the repress of cinnamic acid to cucumber growth by microbial strain].
Cucumber is one of the most important vegetable species. Its continuous planting has become a common practice demand in many areas of China, but an obstacle from continuous planting made sustainable production of this crop to be prohibited. The self-toxic effect was considered as an important negative factor to continuous cropping cucumber. And cinnamic acid was found to be the main substance to cause self-toxic. Strain Ha8, which isolated from waste water estuary in Zhuhai city and has been authenticated as Cellulosimicrobium cellulans, was found to be able to degrade cinnamic acid, benzoic acid, paraaminobenzoic acid and phenol. Its biologic degrading rate to cinnamic acid was 64.1% and its total degrading rate to cinnamic acid was 79.32% . Therefore, strain Ha8 was used to mitigate the growth stress of cucumber caused by cinnamic acid in the research. In the experiment by hydroponic culturing method, it was found that the stem length, root length, stem weight, leaf weight, root weight, numbers of flower and harvest weight of cucumbers were lower than those untreated ones when added 2micromol/L or 10micromol/L cinnamic acid in culturing solution. But when added 10(7)cfu/L of strain Ha8 and 2micromol/L or 10micromol/L cinnamic acid in same culturing solution, these parameters were higher than those treated only by 2mircomol/L or 10micromol/L cinnamic acid. The result shown that strain Ha8 could mitigate the self-toxic effect caused by cinnamic acid. In edaphic culturing experiments, it was found that organic fertilizer mixed with strain Ha8 could mitigate the growth stress of cucumber caused by 100mg/kg cinnamic acid. When added 3mg/kg sterilized organic fertilizer with strain Ha8 (> or = 10(6)cfu/g dry organic fertilizer) in the culturing soil, the result was satisfied. This treatment could not only improve the growth of cucumber, enhance their root dehydrogenase activity and output, promote their nutrition absorption rate, but also adjust the microbial groups in nonrhizospheric soil of cucumber, increase the number of beneficial bacteria and actinomycete, decrease the number of fungi.
Yu
Guo-hui
GH
Zhuhai Agricultural Science Research Center, Zhuhai 519070, China. ygh76411@yahoo.com.cn
Xie
Yin-hua
YH
Chen
Yan-hong
YH
Chen
Yuan-feng
YF
Cheng
Ping
P
D000192:Actinomycetales / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002934:Cinnamates / Q000378:metabolism* / Q000633:toxicity; D018556:Crops, Agricultural / Q000254:growth & development; D018553:Cucumis sativus / Q000254:growth & development* / Q000378:metabolism; D012988:Soil Microbiology; D014871:Water Microbiology
D002934:Cinnamates; C029010:cinnamic acid