title: The combination of ApoCIII, hepatic lipase and hormono sensitive lipase gene polymorphisms suggests an association with susceptibility to gestational hypertension.
abstract: Dyslipidemia and insulin resistance contribute to the endothelial cell dysfunction in hypertensive disorders of pregnancy (HDP) and increase the long-term risk of cardiovascular disease (CVD). The genes linking susceptibility to gestational hypertension (GH) and/or preeclampsia (PE) to the long-term risk of CVD are still unknown. We evaluated the potential association between 14 polymorphisms from six genes involved in lipid metabolism and insulin action and the risk of HDP: namely the lipoprotein lipase (LPL), hepatic lipase (LIPC), hormone sensitive lipase (LIPE), cholesteryl ester transfer protein (CETP), ApoCIII and ApoE gene polymorphisms. Overall, 169 women with HDP [proteinuria (PE) and gestational hypertension without proteinuria (GH)] and 169 controls matched for age and year of delivery were genotyped. Homozygosity of the -514T allele of the -514C > T polymorphism (LIPC gene) decreased the risk of GH (OR = 0.17, CI(95): 0.02-0.76), while there were more -60G carriers of the -60C > G LIPE gene polymorphism (OR = 3.51, CI(95):1.02-12.10) among GH cases, but not in PE cases. The common ApoCIII two-locus -482CC/3238CC genotype was lower in women with GH compared with controls (OR = 0.53, CI(95): 0.3-0.9). The combined frequency of at-risk genotypes was higher in cases of GH compared with controls [one at-risk genotype: OR = 3.38 (95% CI: 0.48-41.8); two or more at-risk genotypes: OR = 7.14 (95% CI: 1.21-92.3, P = 0.01)], suggesting a gene-dose effect. We conclude that the combined effect of LIPC, LIPE and ApoCIII gene polymorphisms may increase the likelihood of GH, but seemingly not of PE.
authors.affiliation: Unité de recherche en périnatalogie, Centre de recherche du CHUQ, Hôpital Saint-François d'Assise, 10, rue de l'Espinay, G1L 3L5, Quebec, QC, Canada.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Bernard
authors.affiliation: Unité de recherche en périnatalogie, Centre de recherche du CHUQ, Hôpital Saint-François d'Assise, 10, rue de l'Espinay, G1L 3L5, Quebec, QC, Canada.|Département de Biologie médicale, Faculté de médecine, Université Laval, Quebec, QC, Canada.
authors.forename: Joël
authors.initials: J
authors.lastname: Girouard
authors.affiliation: Unité de recherche en périnatalogie, Centre de recherche du CHUQ, Hôpital Saint-François d'Assise, 10, rue de l'Espinay, G1L 3L5, Quebec, QC, Canada.|Département de Biologie médicale, Faculté de médecine, Université Laval, Quebec, QC, Canada.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Forest
authors.affiliation: Unité de recherche en périnatalogie, Centre de recherche du CHUQ, Hôpital Saint-François d'Assise, 10, rue de l'Espinay, G1L 3L5, Quebec, QC, Canada. Yves.Giguere@crsfa.ulaval.ca.|Département de Biologie médicale, Faculté de médecine, Université Laval, Quebec, QC, Canada. Yves.Giguere@crsfa.ulaval.ca.
authors.forename: Yves
authors.initials: Y
authors.lastname: Giguère
mesh_terms: D000328:Adult; D053305:Apolipoprotein C-III / Q000235:genetics*; D001057:Apolipoproteins E / Q000235:genetics; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D046110:Hypertension, Pregnancy-Induced / Q000201:enzymology* / Q000235:genetics*; D015810:Linkage Disequilibrium / Q000235:genetics; D008049:Lipase / Q000235:genetics*; D011110:Polymorphism, Genetic; D011225:Pre-Eclampsia / Q000235:genetics; D011247:Pregnancy; D012307:Risk Factors; D002787:Sterol Esterase / Q000235:genetics*
chemical_list: D053305:Apolipoprotein C-III; D001057:Apolipoproteins E; C504173:LIPC protein, human; D002787:Sterol Esterase; D008049:Lipase