title: Inflammatory reaction in rats muscle after implantation of biphasic calcium phosphate micro particles.
abstract: Several studies have shown that macro micro porous bioceramics ectopically implanted promote bone tissue formation. This study aims at investigating the inflammatory response towards biphasic calcium phosphate (BCP) ceramic micro particles. BCP composed of hydroxyapatite (HA) and beta-tricalcium phosphate, HA/beta -TCP ratio of 50/50, were prepared by sintering at 1200 degrees C for 5 h. After crushing, 3 fractions of BCP micro particles < 20, 40-80 and 80-200 micro m were sieved. The micro particles were carefully characterized by using X-ray diffraction (XRD), scanning electron microscopy (SEM) and laser scattering. The inflammatory reactions induced by BCP micro particles implanted in quadriceps muscles of rats for 7, 14 and 21 days were studied by histology (n = 8/group). A fibrous tissue encapsulation of the BCP micro particles implanted in muscle tissue was observed and fibrosis was similar for the 3 groups of micro particles. The comparison of the cellular response indicated that the total number of cells was significantly higher for BCP < 20 micro m than for 40-80 and 80-200 micro m (p < 0.0001). The number of macrophages was relatively higher for the smallest than for the intermediate and largest fractions (p < 0.0001). The relative percentage of giant cells was higher for the intermediate and largest size of particles than for the smallest. The number of lymphocytes was comparable for the 3 fractions and after the 3 delays. Therefore, the BCP micro particles < 20 micro m initiated an inflammatory response which might play an important role in osteogenesis.
authors.affiliation: INSERM U791, Laboratory for Osteoarticular and Dental Tissue Engineering, Place Alexis Ricordeau, 44042 Nantes, France. borhane.fellah@univ-nantes.fr
authors.forename: Borhane H
authors.initials: BH
authors.lastname: Fellah
authors.forename: Nicolas
authors.initials: N
authors.lastname: Josselin
authors.forename: Daniel
authors.initials: D
authors.lastname: Chappard
authors.forename: Pierre
authors.initials: P
authors.lastname: Weiss
authors.forename: Pierre
authors.initials: P
authors.lastname: Layrolle
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects / Q000737:chemistry; D002130:Calcium Phosphates / Q000009:adverse effects* / Q000737:chemistry*; D018929:Cell Culture Techniques / Q000379:methods; D020007:Implants, Experimental / Q000009:adverse effects*; D007249:Inflammation / Q000139:chemically induced* / Q000276:immunology / Q000473:pathology*; D008422:Materials Testing; D011109:Polymethacrylic Acids / Q000009:adverse effects* / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D013329:Structure-Activity Relationship; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D011109:Polymethacrylic Acids; C485829:calcium phosphate, dibasic, anhydrous