Heterogeneous transportation of alpha1B-adrenoceptor in living cells.
The heterogeneous motion of alpha(1B)-adrenoceptor (alpha(1B)-AR) was visualized in living cells with BODIPY-labeled antagonist of AR by single molecule fluorescence microscopy at high spatial resolution. The moving trajectory was reconstructed by precise localization (better than 20 nm) with a least-square fit of a two-dimensional Gaussian point spread function to each single spot. Trajectory analysis revealed two apparent groups of movements: directed motion and hindered motion. The directed motion had speeds higher than 0.1 mum/s. The histogram of diffusion coefficients of the hindered motion showed distinction between the cell membrane and the cytoplasm: the diffusion coefficient was lower near the cell membrane than in the internal cytoplasm, suggesting that alpha(1B)-AR was located or trapped in different networks, which was consistent with the natural distribution of cytoskeleton in living cells. These results suggested that the heterogeneity in the motion of alpha(1B)-AR in living cell might be associated with different localizations of cell skeleton proteins in the cell, which could provide molecular insight of AR regulation in living cells.
Guan
Yinghua
Y
Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, and Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Xu
Ming
M
Liang
Zhangyi
Z
Xu
Ning
N
Lu
Zhizhen
Z
Han
Qide
Q
Zhang
Youyi
Y
Zhao
Xin Sheng
XS
D001665:Binding Sites; D001896:Boron Compounds; D002462:Cell Membrane / Q000378:metabolism*; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism*; D004058:Diffusion; D006801:Humans; D007668:Kidney / Q000166:cytology; D008954:Models, Biological; D011224:Prazosin; D011941:Receptors, Adrenergic; D018340:Receptors, Adrenergic, alpha-1 / Q000378:metabolism*; D014018:Tissue Distribution
C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D011941:Receptors, Adrenergic; D018340:Receptors, Adrenergic, alpha-1; D011224:Prazosin