Adenovirus E1A negatively regulates E1AF, an ets family of the protein.
E1AF was first identified as a transcription factor that binds to enhancer motifs of the adenovirus E1A gene and is thought to be a human homologue of mouse PEA3, one of the ets oncoprotein families. Here we show the effect of E1A on the gene expression and function of E1AF. E1A repressed the activity of E1AF promoter, and the N-terminal region of E1A, which is involved in the oncogenic activity of E1A, was essential for this repression. The ability as a transcription factor of E1AF, as well as those of the other PEA3 subfamily members ER81 and ERM, was also repressed by E1A via the same oncogenic domain. Furthermore, E1AF repressed the transformation activity of E1A cooperating with E1B, whereas the other ets family Ets-1 enhanced this activity. These results suggest that E1AF is one of the targets of E1A.
Takahashi
Akiko
A
Department of Oral Pathobiological Science, Hokkaido University Graduate School of Dental Medicine, N-13 W-7, Kita-ku, Sapporo 060-8586, Japan.
Higashino
Fumihiro
F
Aoyagi
Mariko
M
Nakayama
Masayuki
M
Yanagawa
Aya
A
Hasegawa
Hiroichi
H
Hatta
Mitsutoki
M
Ishida
Setsuko
S
Nakajima
Koichi
K
Totsuka
Yasunori
Y
Shindoh
Masanobu
M
D017500:Adenovirus E1A Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation / Q000235:genetics*; D006801:Humans; D008156:Luciferases / Q000235:genetics; D010957:Plasmids; D011401:Promoter Regions, Genetic; D050784:Proto-Oncogene Protein c-ets-1 / Q000235:genetics*; D051381:Rats
D017500:Adenovirus E1A Proteins; D050784:Proto-Oncogene Protein c-ets-1; D008156:Luciferases