Characterization of microvascular basal lamina damage and blood-brain barrier dysfunction following subarachnoid hemorrhage in rats.
Vasogenic brain edema is one of the major determinants for mortality following subarachnoid hemorrhage (SAH). Although the formation of vasogenic brain edema occurs on the microvascular level by opening of endothelial tight junctions and disruption of the basal lamina, microvascular changes following experimental SAH are poorly characterized. The aim of the present study was therefore to investigate the time course of blood-brain barrier (BBB) dysfunction and basal lamina damage following SAH as a basis for the better understanding of the pathophysiology of SAH. SAH was induced in Sprague-Dawley rats by an endovascular filament. Animals were sacrificed 6, 24, 48, and 72 h thereafter (n=9 per group). Microvascular basal lamina damage was quantified by collagen type IV immunostaining. Western blotting was used to quantify collagen IV protein content and bovine serum albumin (BSA) extravasation as a measure for basal lamina damage and blood-brain barrier disruption, respectively. BSA Western blot revealed significant (p<0.05) BBB opening in the cerebral cortex ipsilateral to the hemorrhage beginning 6 h and peaking 48 h after SAH. Significant (p<0.05) basal lamina damage occurred with gradual increase from 24 to 72 h. Basal lamina damage correlated significantly with BBB dysfunction (r=-0.63; p=0.0001). Microvascular damage as documented by collagen IV degradation and albumin extravasation is a long lasting and ongoing process following SAH. Due to its delayed manner microvascular damage may be prone for therapeutic interventions. However, further investigations are needed to determine the molecular mechanisms responsible for basal lamina degradation and hence damage of the microvasculature following SAH.
Schöller
Karsten
K
Department of Neurosurgery, University of Munich Medical Center-Grosshadern, Ludwig-Maximilians-University, Munich, Germany.
Trinkl
Andreas
A
Klopotowski
Mariusz
M
Thal
Serge C
SC
Plesnila
Nikolaus
N
Trabold
Raimund
R
Hamann
Gerhard F
GF
Schmid-Elsaesser
Robert
R
Zausinger
Stefan
S
D000818:Animals; D001485:Basement Membrane / Q000378:metabolism* / Q000473:pathology; D001812:Blood-Brain Barrier / Q000473:pathology / Q000503:physiopathology*; D002199:Capillary Permeability / Q000502:physiology; D002560:Cerebrovascular Circulation / Q000502:physiology; D024141:Collagen Type IV / Q000378:metabolism; D004195:Disease Models, Animal; D005070:Evans Blue; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013345:Subarachnoid Hemorrhage / Q000150:complications* / Q000473:pathology*; D013997:Time Factors
D024141:Collagen Type IV; D012710:Serum Albumin, Bovine; D005070:Evans Blue