title: Differentially expressed proteins of pathogenic Penicillium marneffei in yeast and mycelial phases.
abstract: Penicillium marneffei is a dimorphic fungus endemic in southeast Asia. The incidence of P. marneffei infection has increased greatly in this region with the spread of human immunodeficiency virus, but the infection routes and pathogenic mechanisms of P. marneffei remain poorly understood. P. marneffei is an opportunistic human pathogen exhibiting a temperature-dependent dimorphic switch. At 25 degrees C it grows as filamentous hyphae, whilst at 37 degrees C it forms uninucleate yeast cells and divides by fission. Dimorphic fungal pathogenicity is frequently associated with the dimorphic switch, but the mechanism that regulates the switch has remained obscure. In this report, two-dimensional difference gel electrophoresis was used to investigate the proteins expressed differentially in the yeast and mycelial phases of a wild-type isolate of P. marneffei. Among thousands of protein molecules displayed, more than 500 showed differential expression between the two phases. In particular, 26 proteins were identified using matrix-assisted laser desorption/ionization time-of-flight MS. Expression of catalase-peroxidase, isocitrate lyase, Hsp90, binding protein and cytochrome P-450 increased significantly in the yeast phase, whereas levels of poly(A) polymerase and SNF22 were reduced.
authors.affiliation: Department of Dermatology, Second Affiliated Hospital, Sun Yat-Sen University, 107 West Yanjiang Road, Guangzhou 510120, China.
authors.forename: Liyan
authors.initials: L
authors.lastname: Xi
authors.affiliation: Department of Dermatology, Second Affiliated Hospital, Sun Yat-Sen University, 107 West Yanjiang Road, Guangzhou 510120, China.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Xu
authors.affiliation: Center for Proteomics, School for Basic Medical Science, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Dermatology, Second Affiliated Hospital, Sun Yat-Sen University, 107 West Yanjiang Road, Guangzhou 510120, China.
authors.forename: Xiqing
authors.initials: X
authors.lastname: Li
authors.affiliation: Center for Proteomics, School for Basic Medical Science, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Center for Proteomics, School for Basic Medical Science, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Mingtao
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Dermatology, Second Affiliated Hospital, Sun Yat-Sen University, 107 West Yanjiang Road, Guangzhou 510120, China.
authors.forename: Junmin
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Microbiology, School for Basic Medical Science, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Mengfeng
authors.initials: M
authors.lastname: Li
mesh_terms: D002675:Child, Preschool; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000096:biosynthesis*; D015966:Gene Expression Regulation, Fungal; D006801:Humans; D013058:Mass Spectrometry; D025282:Mycelium / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D009181:Mycoses / Q000382:microbiology; D010407:Penicillium / Q000166:cytology / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D020543:Proteome / Q000032:analysis
chemical_list: D005656:Fungal Proteins; D020543:Proteome