title: In vivo comparison of the biocompatibility of two root canal sealers implanted into the subcutaneous connective tissue of rats.
abstract: OBJECTIVE
  To evaluate the subcutaneous biocompatibility of 2 root canal sealers.
  
  
  METHODS
  The subcutaneous implant technique recommended by the Fédération Dentaire International (FDI) was used to test Endométhasone and EndoREZ root canal sealers. These materials were placed in Teflon tubes, 1 mm in diameter and 10 mm in length, and implanted into 2 pockets created in the back of 40 Calomys callosus rodents, 20 for each material. Tissue biopsies were collected and histologically examined 15, 30, 60, and 90 days after the implantation procedure. The overall level of the inflammatory tissue response was graded as none, slight, moderate, or severe on the sealer-connective tissue interface at the opening ends of the tubes. The connective tissue response along the lateral wall outside of each tube served as a negative control.
  
  
  RESULTS
  The tissue reaction to the Endométhasone diminished with time. The EndoREZ sealer was highly toxic during all experimental periods.
  
  
  CONCLUSIONS
  Endométhasone root canal sealer presented biocompatibility within the analyzed periods, whereas EndoREZ showed no biocompatible behavior and caused late hypersensitive reaction.
authors.affiliation: Department of Endodontics, College of Dentistry, Universidade Federal de Campo Grande, Campo Grande, Brazil.
authors.forename: Edilson José
authors.initials: EJ
authors.lastname: Zafalon
authors.forename: Marco Aurélio
authors.initials: MA
authors.lastname: Versiani
authors.forename: Cássio José Alves
authors.initials: CJ
authors.lastname: de Souza
authors.forename: Camila Christian Gomes
authors.initials: CC
authors.lastname: Moura
authors.forename: Paula
authors.initials: P
authors.lastname: Dechichi
mesh_terms: D000818:Animals; D003411:Arvicolinae; D003188:Composite Resins / Q000633:toxicity*; D003907:Dexamethasone / Q000633:toxicity*; D004338:Drug Combinations; D005549:Foreign-Body Reaction / Q000139:chemically induced; D005557:Formaldehyde / Q000633:toxicity*; D006854:Hydrocortisone / Q000633:toxicity*; D006968:Hypersensitivity, Delayed / Q000139:chemically induced; D020007:Implants, Experimental; D008297:Male; D008422:Materials Testing; D051381:Rats; D012387:Root Canal Filling Materials / Q000633:toxicity*; D040521:Subcutaneous Tissue / Q000187:drug effects*; D013943:Thymol / Q000031:analogs & derivatives* / Q000633:toxicity
chemical_list: D003188:Composite Resins; D004338:Drug Combinations; C481259:EndoRez; D012387:Root Canal Filling Materials; C011244:corticosteroid methanetriol mixture; D005557:Formaldehyde; D013943:Thymol; D003907:Dexamethasone; D006854:Hydrocortisone