Alpha-lactalbumin suppresses interleukin-6 release after intestinal ischemia/reperfusion via nitric oxide in rats.
Male Sprague-Dawley rats were underwent superior mesenteric artery occlusion for 45 min followed by reperfusion under urethane anesthesia. Each milk proteins (300 mg/ kg) were injected into the duodenum 1 h before the induction of ischemia. IL-6 release in the blood was measured in course of time. Whey protein isolate (WPI) had the suppressive effect against ischemia/reperfusion-induced IL-6 release, while casein and bovine serum albumin had no effect. Both alpha-lactalbumin (aLA) and beta-lactoglobulin (bLG), major components contained in WPI, significantly inhibited the increase of IL-6. However, aLA had more potent activity compared with bLG. Positive correlation between serum aLA level and efficacy for Ischmia/reperfusion was observed. N(G)-nitro-L-arginine methyl ester, nitric oxide (NO) synthase inhibitor, significantly reversed suppressive effect on IL-6 release by aLA. These findings show that aLA has a marked suppressive effect on pro-inflammatory cytokine release, at least in part, through NO-mediated mechanism.
Yamaguchi
M
M
Food Science Institute, Division of Research and Development, Meiji Dairies Corporation, 540, Naruda, Odawara, Kanagawa, 250-0862, Japan. MAKOTO_YAMAGUCHI@MEIJI-MILK.COM
Uchida
M
M
D000818:Animals; D004305:Dose-Response Relationship, Drug; D015850:Interleukin-6 / Q000378:metabolism*; D007768:Lactalbumin / Q000008:administration & dosage / Q000494:pharmacology*; D007782:Lactoglobulins / Q000494:pharmacology*; D008297:Male; D008894:Milk Proteins / Q000494:pharmacology; D009569:Nitric Oxide / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology*
D015850:Interleukin-6; D007782:Lactoglobulins; D008894:Milk Proteins; D009569:Nitric Oxide; D007768:Lactalbumin