title: Efficient gene transfer into murine pancreatic islets using adenovirus vectors.
abstract: We investigated the efficiency of gene transduction into murine pancreatic islets using the adenovirus (Ad) vector. Western blotting analysis showed that mouse pancreatic islets express coxsackievirus and adenovirus receptor, a receptor for Ad. Nevertheless, gene expression after transduction of the Ad vector in vitro was observed only in the periphery of the islets, probably due to physical obstruction against Ad infection of the cells in the inside of islets. Ca(2+)-free treatment before the Ad vector transduction enhanced transduction efficiency in the islets, but not the cells in the inside of islets. The Ad vector transduction through the celiac artery in vivo and then cultivation of islets in vitro resulted in efficient transduction even in the inside of islets. Thus we propose a new strategy for efficient gene transfer to pancreatic beta-cells.
authors.affiliation: Laboratory of Gene Transfer and Regulation, National Institute of Biomedical Innovation, 7-6-8 Saito, Asagi, Ibaraki, Osaka, Japan.
authors.forename: Eri
authors.initials: E
authors.lastname: Mukai
authors.forename: Shimpei
authors.initials: S
authors.lastname: Fujimoto
authors.forename: Fuminori
authors.initials: F
authors.lastname: Sakurai
authors.forename: Kenji
authors.initials: K
authors.lastname: Kawabata
authors.forename: Manabu
authors.initials: M
authors.lastname: Yamashita
authors.forename: Nobuya
authors.initials: N
authors.lastname: Inagaki
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Mizuguchi
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000008:administration & dosage / Q000235:genetics*; D007515:Islets of Langerhans / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL