title: [Expression of matrix metalloproteinases-9 and tissue inhibitors of matrix metalloproteinases-1 in connective tissue of vaginal wall of women with stress urinary incontinence].
abstract: OBJECTIVE
  To study semi-quantitatively mRNA expression of matrix metalloproteinase-9 (MMP-9) and its inhibitor, tissue inhibitor of matrix metalloproteinase-1 (TIMP-1), in vaginal wall connective tissue in women with stress urinary incontinence (SUI) compared to continent controls, and to explore the relationship between MMP-9, TIMP-1 and SUI.
  
  
  METHODS
  Vaginal wall tissues were obtained from 24 women with SUI who were followed-up (12 cases are > 60 years old and 12 cases < or = 60 years old). Seven patients undergoing total hysterectomy for carcinoma in situ of cervix without urinary incontinence served as control group. RNA was extracted and quantified. Semi-quantitative competitive reverse transcription was carried out with oligo-nucleotide primers to quantify MMP-9 and TIMP-1 mRNA expression.
  
  
  RESULTS
  We used GeneSnap to analyze the data. MMP-9 in three groups (> 60, < or = 60 years and control) was 0.56 +/- 0.20, 0.56 +/- 0.19, 0.37 +/- 0.18, significantly decreased (P < 0.05). There was no difference between > 60 and < or = 60 year age groups (P > 0.05). TIMP-1 in three groups was 0.23 +/- 0.11, 0.31 +/- 0.12, 0.41 +/- 0.13, significantly increased (P < 0.05). There was a great difference between > 60 and < or = 60 year age groups in TIMP expression (P > 0.05). The ratio of MMP-9/TIMP-1 in > 60, < or = 60 year age groups and control group was 2.49 +/- 1.82, 1.82 +/- 1.58, 0.90 +/- 1.38, significantly decreased (P < 0.05).
  
  
  CONCLUSIONS
  Stress urinary incontinent women demonstrate a significant increase in MMP-9 mRNA expression and significant decrease in TIMP-1 mRNA expression. In SUI patients, proportion of MMP-9 and TIMP-1 was overbalanced. Both these findings are consistent with increased collagen breakdown and may play an important role in the onset and development of SUI.
authors.affiliation: Department of Obstetrics and Gynecology, Fuzhou General Hospital, Nanjing Military, Chinese People's Liberation Army, Fuzhou 350012, China.
authors.forename: Qun-Fang
authors.initials: QF
authors.lastname: Zhang
authors.forename: Yan-Feng
authors.initials: YF
authors.lastname: Song
authors.forename: Zhong-Yong
authors.initials: ZY
authors.lastname: Zhu
mesh_terms: D000367:Age Factors; D000368:Aged; D003238:Connective Tissue / Q000378:metabolism*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000096:biosynthesis* / Q000235:genetics; D014550:Urinary Incontinence, Stress / Q000378:metabolism* / Q000473:pathology; D014621:Vagina / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D019715:Tissue Inhibitor of Metalloproteinase-1; D020780:Matrix Metalloproteinase 9