title: Gas chromatography-mass spectrometry following pressurized hot water extraction and solid-phase microextraction for quantification of eucalyptol, camphor, and borneol in Chrysanthemum flowers.
abstract: Chrysanthemum flower is a common traditional Chinese medicine (TCM). In this work, pressurized hot water extraction (PHWE) followed by headspace solid-phase microextraction (HS-SPME) and GC-MS was developed for the determination of three main active volatile compounds of eucalyptol, camphor, and borneol in Chrysanthemum flowers from four different growing areas in China by internal standard method. The parameters of PHWE and HS-SPME were optimized. The method was also validated. The results showed that PHWE-SPME-GC-MS is a simple, rapid, efficient, and solvent-free technique for the quantitative determination of eucalyptol, camphor, and borneol in TCMs and is potentially useful for the TCM quality assessment.
authors.affiliation: Zhongshan Hospital, Shanghai Medical College, Fudan University, Shanghai, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Dong
authors.forename: Jiyao
authors.initials: J
authors.lastname: Wang
authors.forename: Chunhui
authors.initials: C
authors.lastname: Deng
authors.forename: Xizhong
authors.initials: X
authors.lastname: Shen
mesh_terms: D001892:Camphanes / Q000032:analysis* / Q000737:chemistry; D002164:Camphor / Q000032:analysis* / Q000737:chemistry; D031202:Chrysanthemum / Q000737:chemistry*; D003511:Cyclohexanols / Q000032:analysis* / Q000737:chemistry; D000077591:Eucalyptol; D035264:Flowers / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D039821:Monoterpenes / Q000032:analysis* / Q000737:chemistry; D052617:Solid Phase Microextraction
chemical_list: D001892:Camphanes; D003511:Cyclohexanols; D039821:Monoterpenes; D002164:Camphor; C022871:isoborneol; D000077591:Eucalyptol