title: Tap and Dbp5, but not Gag, are involved in DR-mediated nuclear export of unspliced Rous sarcoma virus RNA.
abstract: All retroviruses must circumvent cellular restrictions on the export of unspliced RNAs from the nucleus. While the unspliced RNA export pathways for HIV and Mason-Pfizer monkey virus are well characterized, that of Rous sarcoma virus (RSV) is not. We have previously reported that the RSV direct repeat (DR) elements are involved in the cytoplasmic accumulation of unspliced viral RNA. Here, using fluorescent in situ hybridization (FISH), we demonstrate that unspliced viral RNAs bearing a single point mutation (G8863C) in the DR exhibit a restricted cellular localization in and around the nucleus. In contrast, wild type unspliced viral RNA had a diffuse localization throughout the nucleus and cytoplasm. Since the RSV Gag protein has a transient localization in the nucleus, we examined the effect of Gag over-expression on a DR-mediated reporter construct. While Gag did not enhance DR-mediated nuclear export, the dominant-negative expression of two cellular export factors, Tap and Dbp5, inhibited expression of the same reporter construct. Furthermore, FISH studies using the dominant-negative Dbp5 demonstrated that unspliced wild type RSV RNA was retained within the nucleus. Taken together, these results further implicate the DR in nuclear RNA export through interactions with Tap and Dbp5.
authors.affiliation: Department of Biology, Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD 21218, USA.
authors.forename: Jason J
authors.initials: JJ
authors.lastname: LeBlanc
authors.forename: Sabena
authors.initials: S
authors.lastname: Uddowla
authors.forename: Benjamin
authors.initials: B
authors.lastname: Abraham
authors.forename: Sarah
authors.initials: S
authors.lastname: Clatterbuck
authors.forename: Karen L
authors.initials: KL
authors.lastname: Beemon
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D015683:Gene Products, gag / Q000502:physiology; D009154:Mutation; D029543:Nucleocytoplasmic Transport Proteins / Q000502:physiology*; D012326:RNA Splicing; D012367:RNA, Viral / Q000378:metabolism*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D052736:Rous sarcoma virus / Q000235:genetics* / Q000502:physiology; D001357:Sarcoma, Avian / Q000378:metabolism / Q000821:virology; D014157:Transcription Factors / Q000502:physiology*
chemical_list: D015683:Gene Products, gag; D029543:Nucleocytoplasmic Transport Proteins; D012367:RNA, Viral; D014157:Transcription Factors