title: Mapuera virus, a rubulavirus that inhibits interferon signalling in a wide variety of mammalian cells without degrading STATs.
abstract: Mapuera virus (MPRV) is a paramyxovirus that was originally isolated from bats, but its host range remains unknown. It was classified as a member of the genus Rubulavirus on the basis of structural and genetic features. Like other rubulaviruses it encodes a V protein (MPRV/V) that functions as an interferon (IFN) antagonist. Here we show that MPRV/V differs from the IFN antagonists of other rubulaviruses in that it does not induce the proteasomal degradation of STAT proteins, key factors in the IFN signalling cascade. Rather, MPRV/V prevents the nuclear translocation of STATs in response to IFN stimulation and inhibits the formation of the transcription factor complex ISGF3. We also show that MPRV/V blocks IFN signalling in cells from diverse mammalian species and discuss the IFN response as a barrier to cross-species infections.
authors.affiliation: School of Biology, University of St Andrews, Fife KY16 9TS, UK.
authors.forename: K
authors.initials: K
authors.lastname: Hagmaier
authors.affiliation: School of Biology, University of St Andrews, Fife KY16 9TS, UK.
authors.forename: N
authors.initials: N
authors.lastname: Stock
authors.affiliation: School of Biology, University of St Andrews, Fife KY16 9TS, UK.
authors.forename: B
authors.initials: B
authors.lastname: Precious
authors.affiliation: Division of Basic Medical Sciences, St George's, University of London, London SW17 0RE, UK.
authors.forename: K
authors.initials: K
authors.lastname: Childs
authors.affiliation: CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, VIC 3220, Australia.
authors.forename: L-F
authors.initials: LF
authors.lastname: Wang
authors.affiliation: Division of Basic Medical Sciences, St George's, University of London, London SW17 0RE, UK.
authors.forename: S
authors.initials: S
authors.lastname: Goodbourn
authors.affiliation: School of Biology, University of St Andrews, Fife KY16 9TS, UK.
authors.forename: R E
authors.initials: RE
authors.lastname: Randall
mesh_terms: D021581:Active Transport, Cell Nucleus; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D006801:Humans; D050832:Interferon-Stimulated Gene Factor 3 / Q000378:metabolism; D007372:Interferons / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D010766:Phosphorylation; D019213:Rubulavirus / Q000276:immunology*; D050791:STAT Transcription Factors / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology*
chemical_list: D050832:Interferon-Stimulated Gene Factor 3; D050791:STAT Transcription Factors; D014764:Viral Proteins; D007372:Interferons