title: Giardia and Cryptosporidium species and genotypes in coyotes (Canis latrans).
abstract: Feces and duodenal scrapings were collected from 22 coyotes (Canis latrans) killed in managed hunts in northeastern Pennsylvania. Polymerase chain reaction (PCR) methods were used to detect Giardia and Cryptosporidium spp. PCR-amplified fragments of Giardia and Cryptosporidium spp. SSU-rRNA genes were subjected to DNA sequence analysis for species/genotype determination. Seven coyotes (32%) were positive for G. duodenalis: three assemblage C, three assemblage D, and one assemblage B. Six coyotes (27%) were positive for Cryptosporidium spp. One isolate shared 99.7% homology with C. muris, whereas five others (23%) shared 100% homology with C. canis, coyote genotype. This is the first report on multiple genotypes of Giardia spp. in coyotes and on the prevalence of Cryptosporidium spp. genotypes in coyotes.
authors.affiliation: Environmental Microbial Safety Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, U.S. Department of Agriculture, Building 173, BARC-East, 10300 Baltimore Avenue, Beltsville, Maryland, USA, 20705.
authors.forename: James M
authors.initials: JM
authors.lastname: Trout
authors.forename: Mónica
authors.initials: M
authors.lastname: Santín
authors.forename: Ronald
authors.initials: R
authors.lastname: Fayer
mesh_terms: D000818:Animals; D046029:Coyotes / Q000469:parasitology*; D003457:Cryptosporidiosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D003458:Cryptosporidium / Q000235:genetics / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000032:analysis*; D005243:Feces / Q000469:parasitology; D005260:Female; D005784:Gene Amplification; D005838:Genotype; D005872:Giardia / Q000235:genetics / Q000302:isolation & purification; D005873:Giardiasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D008297:Male; D010414:Pennsylvania / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D013045:Species Specificity
chemical_list: D016054:DNA, Protozoan