title: An unmethylated 3' promoter-proximal region is required for efficient transcription initiation.
abstract: The promoter regions of approximately 40% of genes in the human genome are embedded in CpG islands, CpG-rich regions that frequently extend on the order of one kb 3' of the transcription start site (TSS) region. CpGs 3' of the TSS of actively transcribed CpG island promoters typically remain methylation-free, indicating that maintaining promoter-proximal CpGs in an unmethylated state may be important for efficient transcription. Here we utilize recombinase-mediated cassette exchange to introduce a Moloney Murine Leukemia Virus (MoMuLV)-based reporter, in vitro methylated 1 kb downstream of the TSS, into a defined genomic site. In a subset of clones, methylation spreads to within approximately 320 bp of the TSS, yielding a dramatic decrease in transcript level, even though the promoter/TSS region remains unmethylated. Chromatin immunoprecipitation analyses reveal that such promoter-proximal methylation results in loss of RNA polymerase II and TATA-box-binding protein (TBP) binding in the promoter region, suggesting that repression occurs at the level of transcription initiation. While DNA methylation-dependent trimethylation of H3 lysine (K)9 is confined to the intragenic methylated region, the promoter and downstream regions are hypo-acetylated on H3K9/K14. Furthermore, DNase I hypersensitivity and methylase-based single promoter analysis (M-SPA) experiments reveal that a nucleosome is positioned over the unmethylated TATA-box in these clones, indicating that dense DNA methylation downstream of the promoter region is sufficient to alter the chromatin structure of an unmethylated promoter. Based on these observations, we propose that a DNA methylation-free region extending several hundred bases downstream of the TSS may be a prerequisite for efficient transcription initiation. This model provides a biochemical explanation for the typical positioning of TSSs well upstream of the 3' end of the CpG islands in which they are embedded.
authors.affiliation: Department of Medical Genetics, The University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: Ruth
authors.initials: R
authors.lastname: Appanah
authors.forename: David R
authors.initials: DR
authors.lastname: Dickerson
authors.forename: Preeti
authors.initials: P
authors.lastname: Goyal
authors.forename: Mark
authors.initials: M
authors.lastname: Groudine
authors.forename: Matthew C
authors.initials: MC
authors.lastname: Lorincz
mesh_terms: D000107:Acetylation / Q000187:drug effects; D000818:Animals; D002999:Clone Cells; D019175:DNA Methylation / Q000187:drug effects; D018390:Gene Targeting; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D006877:Hydroxamic Acids / Q000494:pharmacology; D051379:Mice; D008969:Molecular Sequence Data; D008979:Moloney murine leukemia virus / Q000235:genetics; D009707:Nucleosomes / Q000187:drug effects / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011485:Protein Binding / Q000187:drug effects; D012319:RNA Polymerase II / Q000378:metabolism; D035181:TATA-Box Binding Protein / Q000378:metabolism; D020079:Terminal Repeat Sequences / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects; D019076:Transgenes
chemical_list: D006657:Histones; D006877:Hydroxamic Acids; D009707:Nucleosomes; D035181:TATA-Box Binding Protein; D049452:Green Fluorescent Proteins; C012589:trichostatin A; D012319:RNA Polymerase II