Anti-asthmatic effects of Perilla seed oil in the guinea pig in vitro and in vivo.
The aim of this study was to investigate the anti-asthmatic effects of Perilla seed oil in vitro and in vivo in sensitized guinea pigs. Aerosolized antigen caused an immediate bronchoconstriction. Perilla seed oil per os inhibited the increase in lung resistance and the decrease in dynamic lung compliance in a dose-dependent manner with an ED50 (95 % confidence interval, CI) of 1.10 (0.98 - 1.24) g/kg and 1.07 (0.94 - 1.22) g/kg, respectively. Infiltration of leukocytes, mononuclear cells, eosinophils and neutrophils induced by inhaling antigen was also inhibited by Perilla seed oil in a dose-dependent manner with an ED50 (95 % CI) of 1.00 (0.86 - 1.15), 1.24 (1.10 - 1.38), 0.63 (0.51 - 0.77) and 0.61 (0.38 - 0.98) g/kg, respectively. Perilla seed oil (5 - 500 microg/mL) inhibited the slow reaction substance of anaphylaxis (SRS-A) release induced by antigen challenge in lung tissue of sensitized guinea pigs. It also inhibited calcium ionophore (A(23187))-induced leukotriene (LT) D4 release from the lung tissue of non-sensitized guinea pigs in a concentration-dependent manner with an IC50 (95 % CI) of 50 (36 - 69) microg/mL. These results indicate that Perilla seed oil may improve lung function in asthma by controlling eicosanoid production and suppressing LT generation.
Deng
Yang-mei
YM
Zhejiang Respiratory Drugs Research Laboratory of State Food and Drug Administration, Medical Science College of Zhejiang University, People's Republic of China.
Xie
Qiang-min
QM
Zhang
Shui-juan
SJ
Yao
Hong-yi
HY
Zhang
Hui
H
D000818:Animals; D000941:Antigens; D001986:Bronchial Spasm / Q000139:chemically induced / Q000517:prevention & control; D001993:Bronchodilator Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D006168:Guinea Pigs; D066298:In Vitro Techniques; D020128:Inhibitory Concentration 50; D008168:Lung / Q000166:cytology / Q000187:drug effects; D008297:Male; D010047:Ovalbumin / Q000276:immunology; D031343:Perilla; D008517:Phytotherapy; D010938:Plant Oils / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D017962:alpha-Linolenic Acid / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use
D000941:Antigens; D001993:Bronchodilator Agents; D010938:Plant Oils; D017962:alpha-Linolenic Acid; C058216:perilla seed oil; D010047:Ovalbumin