Quantitative demonstration of intrathecal synthesis of high affinity immunoglobulin G in herpes simplex encephalitis using affinity-mediated immunoblotting.
Three paired serial samples of CSF and serum (from days 8, 13 and 22) were taken from a patient referred to the National Hospital for Neurology and Neurosurgery with what was duly confirmed as having herpes simplex encephalitis using PCR. The samples were investigated using affinity-mediated immunoblotting followed by incubation with sodium thiocyanate. Digitisation of the blots enabled further analysis. We showed that the clones of antigen-specific IgG, which were produced intrathecally, were of higher relative affinity than polyclonal antigen-specific IgG.
Chapman
Miles D
MD
Department of Neuroimmunology, Institute of Neurology, Queen Square, London, WC1N 3BG, UK. m.chapman@ion.ucl.ac.uk
Thompson
Edward J
EJ
Candler
Paul M
PM
Dale
Russell C
RC
Church
Andrew J
AJ
Giovannoni
Gavin
G
D000368:Aged; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000097:blood / Q000134:cerebrospinal fluid*; D000915:Antibody Affinity; D019540:Area Under Curve; D004279:DNA, Viral; D020803:Encephalitis, Herpes Simplex / Q000134:cerebrospinal fluid* / Q000276:immunology; D005260:Female; D018259:Herpesvirus 1, Human / Q000276:immunology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D015151:Immunoblotting / Q000379:methods*; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood / Q000134:cerebrospinal fluid*; D016133:Polymerase Chain Reaction; D013997:Time Factors
D000914:Antibodies, Viral; D004279:DNA, Viral; D007074:Immunoglobulin G