Cytotoxic and antitumor activity of momilactone B from rice hulls.
The cytotoxic and antitumor activity of methanolic extract of rice hulls (MERH) were evaluated by the MTT-dye reduction assay against human colon cancer cells and the colonic aberrant crypt foci (ACF) assay in 1,2-dimethylhydrazine (DMH)-injected F344 male rats, respectively. MERH was found to be highly cytotoxic, with IC50 values of 0.5 microg/mL in vitro. Forty weeks of MERH supplementation (50 mg/kg of body weight/day) reduced colonic pre-neoplastic ACF formation by 35% (p < 0.01). An active compound, momilactone B, was isolated from MERH by silica gel chromatography, Sephadex LH-20 chromatography, and HPLC. The cytotoxic activity of momilactone B was evaluated by the MTT-dye reduction, lactate dehydrogenase (LDH), and colony-forming ability assays in human colon cancer HT-29 and SW620 cells. The results indicated that momilactone B from rice hulls might be a new candidate for chemotherapeutic agent against human colon cancer.
Kim
Sun-Jung
SJ
Department of Food Science and Biotechnology, Kyungnam University, Masan 631-701, Korea.
Park
Hae-Ryong
HR
Park
Eunju
E
Lee
Seung-Cheol
SC
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D003110:Colonic Neoplasms / Q000517:prevention & control; D004224:Diterpenes / Q000494:pharmacology*; D019073:HT29 Cells; D006801:Humans; D007783:Lactones / Q000494:pharmacology*; D008297:Male; D012275:Oryza / Q000737:chemistry*; D011230:Precancerous Conditions / Q000517:prevention & control; D051381:Rats; D011916:Rats, Inbred F344; D012639:Seeds / Q000737:chemistry*
D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; D007783:Lactones; C477203:momilactone B