Bioanalytical profile of the L-arginine/nitric oxide pathway and its evaluation by capillary electrophoresis.
This review briefly summarizes recent progress in fundamental understanding and analytical profiling of the L-arginine/nitric oxide (NO) pathway. It focuses on key analytical references of NO actions and the experimental acquisition of these references in vivo, with capillary electrophoresis (CE) and high-performance capillary electrophoresis (HPCE) comprising one of the most flexible and technologically promising analytical platform for comprehensive high-resolution profiling of NO-related metabolites. Another aim of this review is to express demands and bridge efforts of experimental biologists, medical professionals and chemical analysis-oriented scientists who strive to understand evolution and physiological roles of NO and to develop analytical methods for use in biology and medicine.
Boudko
Dmitri Y
DY
The Whitney Laboratory for Marine Bioscience, 9505 Ocean Shore Blvd., St. Augustine, FL 32080, USA. boudko@whitney.ufl.edu
D000818:Animals; D001120:Arginine / Q000032:analysis* / Q000378:metabolism; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D009569:Nitric Oxide / Q000032:analysis* / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism
D009569:Nitric Oxide; D001120:Arginine; D019001:Nitric Oxide Synthase