An absorption study of dietary administered acrylamide in swine.
Acrylamide is a food toxicant suspected to be carcinogenic to humans. It is formed in the heat processing of carbohydrate-rich food. A current issue in food safety is whether acrylamide actually represents a risk for human health. At present, available information is insufficient to reach any conclusions. Inter alias, a still unclear matter is the fraction of acrylamide ingested by food that is absorbed and metabolized. This study compared the in vivo relative absorption of acrylamide formed in cooked food with that of the pure compound dissolved in drinking water using the pig (25 Italian Large White females) as the animal model. Acrylamide intakes of about 0.8 and 8 microg kg(-1) pig body wt day(-1) equal to one and ten times, respectively, the maximum average acrylamide daily intake for humans from the diet (expressed on a body wt basis) in industrialized countries, were chosen for the study. Adducts with the N-terminal valine of haemoglobin formed by acrylamide and its epoxide metabolite glycidamide, were used as exposure markers. Analyses were carried out by gas chromatography/mass spectrometry following in-house method validation. Both for the low and the high dose regimen, the glycidamide adduct levels in swine globins were lower of the limit of quantification of the method. As concerns acrylamide adducts, it was found that the relative absorption of acrylamide from feed and water was the same and that there is a direct proportionality between the adduct concentration and acrylamide intake.
Aureli
Federica
F
National Center for Food Quality and Risk Assessment, Istituto Superiore di Sanità, viale Regina Elena 299, 00161 Rome, Italy.
Di Pasquale
Mauro
M
Lucchetti
Dario
D
Aureli
Paolo
P
Coni
Ettore
E
D020106:Acrylamide / Q000008:administration & dosage / Q000493:pharmacokinetics*; D000284:Administration, Oral; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002274:Carcinogens, Environmental / Q000008:administration & dosage / Q000493:pharmacokinetics*; D003296:Cooking; D004032:Diet; D004305:Dose-Response Relationship, Drug; D004326:Drinking; D004852:Epoxy Compounds / Q000737:chemistry / Q000378:metabolism; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006454:Hemoglobins / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D013552:Swine; D014633:Valine / Q000031:analogs & derivatives / Q000097:blood
D015415:Biomarkers; D002274:Carcinogens, Environmental; D004852:Epoxy Compounds; D006454:Hemoglobins; D020106:Acrylamide; C071834:glycidamide; D014633:Valine