title: Isolation and characterization of actinomycetes strains that produce phospholipase D having high transphosphatidylation activity.
abstract: The present study was conducted to screen microorganisms that produce phospholipase D (PLD), and we especially focused on the strains having high transphosphatidylation activity. Eighty bacterial strains were isolated from soil samples by a screening method utilizing a preliminary selection medium with phosphatidylcholine (PC) as the sole carbon source. The culture supernatants were then assayed for PLD activity. The finding of dual PLD activities in cultures revealed that the hydrolytic and transphosphatidylation activities were correlated. Consequently, six strains were selected as stably producing PLD enzyme(s) during continuous subcultures. The culture supernatants of selected strains synthesized phosphatidylglycerol, phosphatidylserine and phosphatidylethanolamine from PC with high conversion rates. These isolated strains will be made available to carry out phospholipid modification through the efficient transphosphatidylation activity of the PLD that they produce.
authors.affiliation: Department of Applied Biology and Chemistry, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
authors.forename: Yozo
authors.initials: Y
authors.lastname: Nakazawa
authors.forename: Masataka
authors.initials: M
authors.lastname: Uchino
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Sagane
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Sato
authors.forename: Katsumi
authors.initials: K
authors.lastname: Takano
mesh_terms: D039903:Actinobacteria / Q000737:chemistry / Q000201:enzymology* / Q000302:isolation & purification* / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D010739:Phospholipase D / Q000737:chemistry / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D012988:Soil Microbiology
chemical_list: D001426:Bacterial Proteins; D010743:Phospholipids; D010739:Phospholipase D