Gastrulation of Gastrotheca riobambae in comparison with other frogs.
Blastopore formation, the embryonic disk, archenteron and notochord elongation, and Brachyury expression in the marsupial frog Gastrotheca riobambae was compared with embryos of Xenopus laevis and of the dendrobatids Colostethus machalilla and Epipedobates anthonyi. In contrast with X. laevis embryos, the blastopore closes before elongation of the archenteron and notochord in the embryos of G. riobambae and of the dendrobatid frogs. Moreover, the circumblastoporal collar (CBC) thickens due to the accumulation of involuted cells. An embryonic disk, however, is formed only in the G. riobambae gastrula. We differentiate three gastrulation patterns according to the speed of development: In X. laevis, elongation of the archenteron and notochord begin in the early to mid gastrula, whereas in the dendrobatids C. machalilla and E. anthonyi the archenteron elongates at mid gastrula and the notochord elongates after gastrulation. In G. riobambae, only involution takes place during gastrulation. Archenteron and notochord elongation occur in the post gastrula. In the non-aquatic reproducing frogs, the margin of the archenteron expands anisotropically, resulting in an apparent displacement of the CBC from a medial to a posterior location, resembling the displacement of Hensen's node in the chick and mouse. The differences detected indicate that amphibian gastrulation is modular.
Moya
Iván M
IM
Pontificia Universidad Católica del Ecuador, Escuela de Ciencias Biológicas, Apartado 17-01-2184, Avenida 12 de Octubre y Robles, Quito, Ecuador.
Alarcón
Ingrid
I
del Pino
Eugenia M
EM
D000818:Animals; D001001:Anura / Q000196:embryology* / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D005326:Fetal Proteins / Q000096:biosynthesis; D005775:Gastrula / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D009672:Notochord / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D020825:T-Box Domain Proteins / Q000096:biosynthesis; D000098908:Brachyury Protein
D005326:Fetal Proteins; D020825:T-Box Domain Proteins; D000098908:Brachyury Protein