Effects of dust storm PM2.5 on cell proliferation and cell cycle in human lung fibroblasts.
Reports on the effects of PM2.5 from dust storm on lung cells are limited. We compared the effects of PM2.5 collected in dust storm days (dust storm PM2.5) with that in sunshiny and non-dust storm days (normal PM2.5) on cell proliferation and cell cycle in human lung fibroblasts. Our results showed that both dust storm and normal PM2.5 had biphasic effects on cell proliferation, namely, stimulated cell proliferation at lower concentrations while inhibited it at higher concentrations. On the contrary, the organic and inorganic extracts from dust storm and normal PM2.5 significantly inhibited the proliferation in human lung fibroblasts at the concentrations corresponding to their mass contents in PM2.5 samples. The flow cytometry showed that the number of cells in G2/M phase increased significantly after treatment with the dust storm and normal PM2.5. The inorganic and organic extracts from PM2.5, however, induced cell arrest in S phase and G0/G1 phase, respectively. It seems that the biphasic effects of both dust storm and normal PM2.5 on cell proliferation may not be related to their inorganic or organic extractable components.
Deng
Furong
F
Department of Occupational and Environmental Health Sciences, Peking University School of Public Health, Beijing 100083, China.
Guo
Xinbiao
X
Liu
Hong
H
Fang
Xin
X
Yang
Minjuan
M
Chen
Wei
W
D002452:Cell Count; D002453:Cell Cycle / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004391:Dust / Q000032:analysis; D005347:Fibroblasts / Q000187:drug effects*; D005434:Flow Cytometry; D006801:Humans; D008168:Lung / Q000166:cytology* / Q000187:drug effects; D052638:Particulate Matter / Q000032:analysis / Q000633:toxicity*; D013778:Tetrazolium Salts; D013844:Thiazoles
D004391:Dust; D052638:Particulate Matter; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue