Electrochemical detection of DNA hybridization by means of osmium tetroxide complexes and protective oligonucleotides.
We have utilized protective oligonucleotides to modify DNA fragments with osmium tetroxide complexes without compromising their ability to hybridize with immobilized thiol-linked probe-SAMs on gold electrodes. Due to reversible voltammetric signals of Os(VI/IV), this method allowed sensitive electrochemical hybridization detection of short (25 bases) and long (120 bases) thymine-containing DNA targets. The detection limit was 3.2 nM for the long target. We found an optimum 40 degrees C hybridization temperature for the short target. No interference by noncomplementary DNA was observed. At least 10 repetitive hybridization experiments at the same probe-SAM were possible with thermal denaturation in between. Such use of protective strands could be useful also for other types of DNA recognition and even for other DNA-modifying agents. Moreover, it is possible to produce electrochemically active oligonucleotides (targets and reporter probes) in ones own laboratory in a simple way.
Flechsig
Gerd-Uwe
GU
Department of Chemistry, University of Rostock, Dr.-Lorenz-Weg 1, D-18059 Rostock, Germany. gerd-uwe.flechsig@uni-rostock.de
Reske
Thomas
T
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods*; D009841:Oligonucleotides / Q000737:chemistry*; D009993:Osmium Tetroxide / Q000737:chemistry*; D011199:Potentiometry / Q000379:methods*; D013941:Thymine / Q000032:analysis
D009841:Oligonucleotides; D004247:DNA; D009993:Osmium Tetroxide; D013941:Thymine