title: PSF is an IbeA-binding protein contributing to meningitic Escherichia coli K1 invasion of human brain microvascular endothelial cells.
abstract: During the development of Escherichia coli K1 meningitis, interaction between E. coli invasion protein IbeA and surface protein(s) on human brain microvascular endothelial cells (HBMEC) is required for invasion and IbeA-mediated signaling. Here, an IbeA-binding protein was identified as polypyrimidine tract-binding protein (PTB)-associated splicing factor (PSF). The specific binding was confirmed by ligand overlay assay. The cell surface-expressed PSF was verified by the confocal microscopy. Recombinant PSF blocked E. coli K1 invasion of HBMEC effectively. Overexpression of PSF in the lentivirus-transducted HBMEC significantly enhanced E. coli K1 invasion. These results suggest that IbeA interacts with PSF for the E. coli K1 invasion of HBMEC.
authors.affiliation: Saban Research Institute, Childrens Hospital Los Angeles, Los Angeles, CA 90027, USA.
authors.forename: Yanming
authors.initials: Y
authors.lastname: Zou
authors.forename: Lina
authors.initials: L
authors.lastname: He
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Wu
authors.forename: Hong
authors.initials: H
authors.lastname: Cao
authors.forename: Zhi-Hua
authors.initials: ZH
authors.lastname: Xie
authors.forename: Yannan
authors.initials: Y
authors.lastname: Ouyang
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Ambrose
authors.initials: A
authors.lastname: Jong
authors.forename: Sheng-He
authors.initials: SH
authors.lastname: Huang
mesh_terms: D000595:Amino Acid Sequence; D001812:Blood-Brain Barrier; D001921:Brain / Q000098:blood supply / Q000382:microbiology*; D002460:Cell Line; D042783:Endothelial Cells / Q000382:microbiology*; D004926:Escherichia coli / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D000072670:PTB-Associated Splicing Factor; D011485:Protein Binding; D016601:RNA-Binding Proteins / Q000378:metabolism*
chemical_list: C096350:CusC protein, E coli; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D000072670:PTB-Associated Splicing Factor; D016601:RNA-Binding Proteins