title: Imaging receptor microdomains on leukocyte subsets in live mice.
abstract: We present a simple method to identify the recruitment of leukocyte subsets and determine concurrent surface-receptor clustering in live mice. We show that CD45+ F4/80- Gr-1+ neutrophils are robustly recruited in surgery-activated cremasteric venules, whereas adherent CD45+ B220+ B lymphocytes were dominant in bone marrow venules. Most adherent Gr-1+ leukocytes are not firmly stationary but actively migrate on TNF-alpha-activated cremasteric venular endothelium and exhibit marked polarization of surface PSGL-1, but not LFA-1, to the trailing edge.
authors.affiliation: Department of Medicine and Immunobiology Center, Mount Sinai School of Medicine, One Gustave L. Levy Place, New York, New York 10029, USA.
authors.forename: Elaine Y
authors.initials: EY
authors.lastname: Chiang
authors.forename: Andrés
authors.initials: A
authors.lastname: Hidalgo
authors.forename: Jungshan
authors.initials: J
authors.lastname: Chang
authors.forename: Paul S
authors.initials: PS
authors.lastname: Frenette
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000276:immunology*; D016231:Fluorescence Polarization Immunoassay / Q000379:methods*; D007962:Leukocytes / Q000166:cytology* / Q000276:immunology*; D021962:Membrane Microdomains / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*
chemical_list: D000954:Antigens, Surface