Molecular characterization of Clostridium difficile clinical isolates in a geriatric hospital.
The discriminatory potential of a combination of various typing methods was evaluated on a set of 21 Clostridium difficile isolates obtained from symptomatic patients hospitalized in a geriatric unit and 7 non-toxigenic isolates from the same hospital. Isolates were firstly serotyped and toxinotyped. Of the 28 isolates, 19 belonged to serogroup A. PCR-ribotyping and PCR-RFLP on the fliC and slpA genes were then applied to these 19 isolates. The results suggest that the combination of PCR-ribotyping with PCR-RFLP analysis of slpA could be more discriminatory and suitable for studying C. difficile epidemiology.
Poilane
Isabelle
I
AP-HP, Hôpital Jean Verdier-René Muret, Service de Microbiologie, Bondy, France.
Humeniuk-Ainouz
Christel
C
AP-HP, Hôpital Jean Verdier-René Muret, Service de Microbiologie, Bondy, France.
Durand
Isabelle
I
AP-HP, Hôpital Jean Verdier-René Muret, Service de Microbiologie, Bondy, France.
Janoir
Claire
C
Université de Paris-Sud-XI, Faculté de Pharmacie, USC INRA EA3534, Châtenay-Malabry, France.
Cruaud
Philippe
P
AP-HP, Hôpital Jean Verdier-René Muret, Service de Microbiologie, Bondy, France.
Delmée
Michel
M
Université Catholique de Louvain, Unité de Microbiologie, Brussels, Belgium.
Popoff
Michel R
MR
Institut Pasteur, Unité des Bactéries Anaérobies et du Botulisme, Paris, France.
Collignon
Anne
A
Université de Paris-Sud-XI, Faculté de Pharmacie, USC INRA EA3534, Châtenay-Malabry, France.|AP-HP, Hôpital Jean Verdier-René Muret, Service de Microbiologie, Bondy, France.
D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000032:analysis; D015373:Bacterial Typing Techniques / Q000379:methods*; D016360:Clostridioides difficile / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D003428:Cross Infection / Q000382:microbiology*; D004761:Enterocolitis, Pseudomembranous / Q000382:microbiology*; D005602:France; D005838:Genotype; D005853:Geriatrics; D006782:Hospitals, Special; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods; D010641:Phenotype; D012150:Polymorphism, Restriction Fragment Length; D021521:Ribotyping; D012703:Serotyping
D001426:Bacterial Proteins; D001427:Bacterial Toxins; C070683:surface layer protein A, Bacteria