Solid-state 31P NMR spectroscopy of precipitated guanine nucleotide-binding protein Ras in complexes with its effector molecules Raf kinase and RalGDS.
Liquid-state 31P NMR spectroscopy is a well-established method for the study of guanine nucleotide-binding proteins (GNB proteins) such as the proto-oncogene Ras. Solid-state 31P NMR spectroscopy could meanwhile also be used to study microcrystalline samples of Ras as well as its partial loss-of-function mutants Ras(T35S) and Ras(T35A). However, solid-state NMR studies of the latter mutants in complex with effector molecules such as RalGDS or Raf kinase were so far prevented, since it has been impossible to crystallize these complexes yet. The aim of the present contribution is to make such complexes accessible to solid-state 31P NMR spectroscopy by the application of precipitation methods. The complex formed by Ras(T35S) and Raf kinase is preserved during precipitation. In contrast, the weakly bound complex of Ras(T35S) with RalGDS is dissociated or at least perturbed by the precipitation procedure. Solid-state 31P NMR experiments on precipitates of these complexes deliver spectra of high resolution and signal-to-noise ratio which allows the application of two-dimensional techniques. Precipitates prepared using polyethylene glycol 6000 (PEG) as precipitant were found to exhibit spectra of maximum resolution and signal-to-noise ratio. Interestingly, the 31P signal due to the alpha-phosphate of GppNHp bound to Ras(T35S) in crystalline samples or aged precipitates has a significantly different isotropic chemical shift than in the liquid state or in freshly prepared precipitates. This directly indicates that the crystal structure differs from the equilibrium solution structure at least in the neighborhood of the alpha-phosphate group.
Ader
Christian
C
Institute of Biophysics and Physical Biochemistry, University of Regensburg, D-93040 Regensburg, Germany.
Spoerner
Michael
M
Kalbitzer
Hans Robert
HR
Brunner
Eike
E
D006150:Guanine Nucleotides / Q000737:chemistry*; D009154:Mutation; D019906:Nuclear Magnetic Resonance, Biomolecular; D011092:Polyethylene Glycols; D011485:Protein Binding; D048490:raf Kinases / Q000737:chemistry*; D020726:ral Guanine Nucleotide Exchange Factor / Q000737:chemistry*; D018631:ras Proteins / Q000737:chemistry* / Q000235:genetics
D006150:Guanine Nucleotides; D020726:ral Guanine Nucleotide Exchange Factor; D011092:Polyethylene Glycols; D048490:raf Kinases; D018631:ras Proteins