title: Transcriptional regulation of human chromatin assembly factor ASF1.
abstract: Antisilencing function 1 (ASF1) is a conserved histone chaperone implicated in nucleosome assembly, transcriptional silencing, and the cellular response to DNA damage. Here, we report the identification of human ASF1B, but not ASF1A, as a direct transcriptional target of transcription factor E2F1. We demonstrated that overexpression of E2F1 by adenoviral-mediated gene transfer upregulated ASF1B mRNA expression in HeLa cells. Analysis of human ASF1B promoter constructs showed that an E2F-responsive sequence was necessary for E2F1-induced activation of the ASF1B gene transcription. Oligonucleotides including an E2F consensus sequence were specifically bound by E2F1 protein in vitro. Chromatin immunoprecipitation analysis demonstrated that E2F1 bound to an E2F-responsive sequence of the human ASF1B gene. Among the members of the E2F family, E2F1 to E2F5, but not E2F6, activated the ASF1B reporter construct. Sp1 and NFYA failed to induce the activity of the ASF1A and ASF1B promoter. ASF1A and ASF1B mRNA were upregulated by serum stimulation. Taken together, our results suggest that the expression of human ASF1A and ASF1B are upregulated followed by cell proliferation signal, but that of ASF1B is uniquely regulated by transcription factors E2F during cell cycle progression.
authors.affiliation: Department of Life Sciences, School of Agriculture, Meiji University, Kanagawa, Japan.
authors.forename: Reiko
authors.initials: R
authors.lastname: Hayashi
authors.forename: Yuya
authors.initials: Y
authors.lastname: Goto
authors.forename: Rena
authors.initials: R
authors.lastname: Tanaka
authors.forename: Kaori
authors.initials: K
authors.lastname: Oonogi
authors.forename: Masaharu
authors.initials: M
authors.lastname: Hisasue
authors.forename: Kenichi
authors.initials: K
authors.lastname: Yoshida
mesh_terms: D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000096:biosynthesis* / Q000235:genetics; D049109:Cell Proliferation; D050687:E2F1 Transcription Factor / Q000235:genetics / Q000502:physiology*; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D018832:Molecular Chaperones; D011401:Promoter Regions, Genetic; D050436:Regulatory Elements, Transcriptional
chemical_list: C491641:ASF1A protein, human; C491640:ASF1B protein, human; D018797:Cell Cycle Proteins; D050687:E2F1 Transcription Factor; D018832:Molecular Chaperones