title: Capsaicin induces cofilin dephosphorylation in human intestinal cells: the triggering role of cofilin in tight-junction signaling.
abstract: Previously, we demonstrated that capsaicin induces tight-junction (TJ) opening in human intestinal Caco-2 cells. In order to clarify the mechanism underlying the TJ opening action of capsaicin, we performed a proteomics study on capsaicin-treated Caco-2 cells. Phosphorylated cofilin was decreased significantly by capsaicin treatment. In addition, capsaicin induced Ca2+ influx in Caco-2 cells and there was a clear correlation between Ca2+) influx and cofilin dephosphorylation (activation). The Ca2+-chelating reagent EGTA blocked the cofilin dephosphorylation induced by both capsaicin and ionomycin, suggesting that the dephosphorylation was mediated by Ca2+ influx. Finally, transepithelial electrical resistance measurements showed that TJ opening accompanied cofilin dephosphorylation. Our data suggest that TJ opening is mediated by cofilin dephosphorylation, which is caused by capsaicin stimuli, including Ca2+ influx. This is the first report of capsaicin action via the dephosphorylation of cofilin in human intestinal cells.
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Nagumo
authors.forename: Junkyu
authors.initials: J
authors.lastname: Han
authors.forename: Mitsue
authors.initials: M
authors.lastname: Arimoto
authors.forename: Hiroko
authors.initials: H
authors.lastname: Isoda
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Tanaka
mesh_terms: D015153:Blotting, Western; D018938:Caco-2 Cells; D002118:Calcium / Q000378:metabolism; D002211:Capsaicin / Q000494:pharmacology*; D051338:Cofilin 1 / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology / Q000187:drug effects*; D017136:Ion Transport; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D019108:Tight Junctions / Q000187:drug effects* / Q000378:metabolism
chemical_list: D051338:Cofilin 1; D010750:Phosphoproteins; D002211:Capsaicin; D002118:Calcium