Omega-3 polyunsaturated fatty acids improve host response in chronic Pseudomonas aeruginosa lung infection in mice.
Pseudomonas aeruginosa is a gram-negative bacilli frequently encountered in human pathology. This pathogen is involved in a large number of nosocomial infections and chronic diseases. Herein we investigated the effects of polyunsaturated fatty acids (PUFA) in chronic Pseudomonas aeruginosa lung infection. C57BL/6 mice were fed for 5 wk with specifically designed diets with high contents in either omega-3 (omega-3) or omega-6 PUFA and compared to a control diet. P. aeruginosa included in agarose beads was then instilled intratracheally, and the animals were studied for 7 days. On the 4th day, the mice fed with the omega-3 diet had a higher lean body mass gain and a lower omega-6:omega-3 ratio of fatty acids extracted from the lung tissue compared with the other groups (P < 0.05). The omega-3 group had the lowest mortality. Distal alveolar fluid clearance (DAFC) as well as the inflammatory response and the cellular recruitment were higher in the omega-3 group on the 4th day. The effect on DAFC was independent of alpha-epithelial Na(+) channels (alpha-ENaC), beta-ENaC, and alpha(1)-Na-K-ATPase mRNA expressions, which were not altered by the different diets. In conclusion, a diet enriched in omega-3 PUFA can change lung membrane composition and improve survival in chronic pneumonia. This effect on survival is probably multifactorial involving the increased DAFC capacity as well as the optimization of the initial inflammatory response. This work suggests that a better control of the omega-6/omega-3 PUFA balance may represent an interesting target in the prevention and/or control of P. aeruginosa infection in patients.
Pierre
Maud
M
Faculté de Médecine, Hôpital Jeanne de Flandre, Université de Lille and CHRU de Lille, France.
Husson
Marie-Odile
MO
Le Berre
Rozenn
R
Desseyn
Jean-Luc
JL
Galabert
Claude
C
Béghin
Laurent
L
Beermann
Christopher
C
Dagenais
André
A
Berthiaume
Yves
Y
Cardinaud
Bruno
B
Barbry
Pascal
P
Gottrand
Frédéric
F
Guery
Benoît P
BP
D000821:Animal Feed; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D053503:Epithelial Sodium Channels / Q000235:genetics; D015633:Extravascular Lung Water / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000276:immunology; D018410:Pneumonia, Bacterial / Q000188:drug therapy* / Q000276:immunology; D011552:Pseudomonas Infections / Q000188:drug therapy* / Q000276:immunology; D011550:Pseudomonas aeruginosa; D011650:Pulmonary Alveoli / Q000378:metabolism / Q000382:microbiology; D012333:RNA, Messenger / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D015431:Weight Loss
D053503:Epithelial Sodium Channels; D015525:Fatty Acids, Omega-3; D015850:Interleukin-6; D012333:RNA, Messenger; C507049:Scnn1b protein, mouse; D014409:Tumor Necrosis Factor-alpha; D000254:Sodium-Potassium-Exchanging ATPase