title: CD5-positive mucosa-associated lymphoid tissue (MALT) lymphoma of ocular adnexal origin: usefulness of fluorescence in situ hybridization for distinction between mantle cell lymphoma and MALT lymphoma.
abstract: Extranodal marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue type (MALT lymphoma) usually lacks CD5 expression. Herein is described two cases of CD5-positive MALT lymphoma of ocular adnexal origin. The differential diagnosis between CD5-positive MALT lymphoma and mantle cell lymphoma (MCL), notably cyclin D1-negative MCL, was difficult because both cases consisted histologically of small to medium-sized cells with diffuse or vaguely nodular growth pattern, and the neoplastic cells were positive for CD5 and negative for cyclin D1. Somatic mutation analysis of the immunoglobulin heavy chain variable region (VH) gene in case 1 found a relatively higher mutation frequency (5.0%), which was not definitive to rule out MCL. Interphase fluorescence in situ hybridization (FISH) on paraffin-embedded section using IgH/cyclin D1 (CCND1) probe showed that in both cases there was no molecular evidence of t(11;14), finally leading to the diagnosis of CD5-positive MALT lymphoma. Although the present two patients had no recurrence over 34 months after initial diagnosis, careful observation is needed because the clinicopathological significance of MALT lymphoma with this rare phenotype remains obscure.
authors.affiliation: Department of Pathology, Fukushima Medical University, School of Medicine, Fukushima, Japan. tasakika@fmu.ac.jp
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Tasaki
authors.forename: Akiko
authors.initials: A
authors.lastname: Shichishima
authors.forename: Minoru
authors.initials: M
authors.lastname: Furuta
authors.forename: Sayaka
authors.initials: S
authors.lastname: Yoshida
authors.forename: Naoya
authors.initials: N
authors.lastname: Nakamura
authors.forename: Masafumi
authors.initials: M
authors.lastname: Abe
mesh_terms: D000368:Aged; D018956:CD5 Antigens / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D004273:DNA, Neoplasm / Q000235:genetics; D003937:Diagnosis, Differential; D005134:Eye Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics / Q000378:metabolism; D017404:In Situ Hybridization, Fluorescence; D018442:Lymphoma, B-Cell, Marginal Zone / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D020522:Lymphoma, Mantle-Cell / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D018294:Neoplasms, Adnexal and Skin Appendage / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism
chemical_list: D018956:CD5 Antigens; D004273:DNA, Neoplasm; D007143:Immunoglobulin Heavy Chains; D019938:Cyclin D1