[Anti-mite activities of 25 kinds of traditional Chinese medicines for Demodex folliculorum].
OBJECTIVE
To explore traditional Chinese medicines (TCMs) with anti-mite activities for Dernodex folliculorum in vitro.


METHODS
Active Demodex folliculorum were selected from patients with moderate to severe Demodex infestation. A total of 25 TCMs were individually extracted with 80% ethanol by heat under reflux, and then prepared to be concentration of 200 mg/ml. The extractions were used individually in mite-killing experiment in vitro to judge anti-mite activity by observing time of killing mites. Physiological saline and Radix Stemonae extraction were selected as blank control and positive control, respectively.


RESULTS
The TCMs with average time of killing mites below 5 min were Cortex Phellodendri; Herba Taraxac, Herba Agrimoniae, Semen Hydnocarpi, Pericarpium Citri Reticulatae, Rhizoma Dioscoreae Hypoglaucae, Flos Genkwa and Radix Stemona. Among them, time with Cortex phellodendri, Herba Taraxac, Herba Agrimoniae, Semen Hydnocarpi and Pericarpium Citri Reticulatae was statistically shorter than that with Radix Stemonae (P < 0.05).


CONCLUSIONS
Cortex Phellodendri, Herba Taraxac and Herba Agrimoniae et al. had remarkable anti-mite activities to Demodex folliculorum.
Tian
Ye
Y
School of Medicine, Anhui University of Science and Technology, Huainan 232001, China. yetian001@126.com
Li
Chao-pin
CP
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D018859:Hair Follicle / Q000469:parasitology; D006801:Humans; D007306:Insecticides / Q000494:pharmacology*; D008924:Mite Infestations / Q000469:parasitology*; D008925:Mites / Q000187:drug effects*; D010946:Plants, Medicinal / Q000737:chemistry*; D012867:Skin / Q000469:parasitology; D013995:Time
D004365:Drugs, Chinese Herbal; D007306:Insecticides