Nitric oxide and oxygen radical attack on GDP-dissociation inhibitor 2 (GDI-2) in spinal cord injury of the rat.
Protein oxidation and nitration have been described during spinal cord injury (SCI) in animal models. Herein, mass spectrometry unambiguously identified GDP-dissociation inhibitor-2 (GDI-2) in SCI with post-translational modifications of 3-aminotyrosine (8 h post-injury) and an acrolein adduct of GDI-2 (72 h post-injury). On the basis of mass spectrometry evidence, we conclude that lipid-peroxidation and protein nitration do take place on an important signalling protein that may be prevented by specific experimental therapeutic interventions.
John
Julius Paul Pradeep
JP
Department of Pediatrics, Medical University of Vienna, Vienna, Austria.
Pintsov
Oliver
O
Petter-Puchner
Alexander
A
Redl
Heinz
H
Pollak
Arnold
A
Chen
Wei-Qiang
WQ
Lubec
Gert
G
D000171:Acrolein / Q000032:analysis; D000595:Amino Acid Sequence; D000818:Animals; D020730:Guanine Nucleotide Dissociation Inhibitors / Q000737:chemistry / Q000378:metabolism*; D015227:Lipid Peroxidation; D008297:Male; D008969:Molecular Sequence Data; D009569:Nitric Oxide / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011499:Protein Processing, Post-Translational; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013119:Spinal Cord Injuries / Q000378:metabolism*; D014443:Tyrosine / Q000031:analogs & derivatives / Q000032:analysis
C521203:Gdi2 protein, rat; D020730:Guanine Nucleotide Dissociation Inhibitors; D017382:Reactive Oxygen Species; C019253:3-aminotyrosine; D009569:Nitric Oxide; D014443:Tyrosine; D000171:Acrolein