In vitro and in vivo antiallergic effects of Glycyrrhiza glabra and its components.
Licorice (Glycyrrhiza glabra L., Leguminosae) is frequently used in traditional medicine to treat inflammatory and allergic diseases. In this study, the main components (glycyrrhizin, 18beta-glycyrrhetinic acid, isoliquiritin, and liquiritigenin) were isolated from licorice, and their anti-allergic effects, such as antiscratching behavior and IgE production-inhibitory activity, were evaluated both in vitro and in vivo. Liquiritigenin and 18beta-glycyrrhetinic acid most potently inhibited the degranulation of RBL-2H3 cells induced by IgE with the antigen (DNP-HSA) and rat peritoneal mast cells induced by compound 48/80. Liquiritigenin and 18beta-glycyrrhetinic acid potently inhibited the passive cutaneous anaphylactic reaction as well as the scratching behavior in mice induced by compound 48/80. These components inhibited the production of IgE in ovalbumin-induced asthma mice but liquiritigenin had little effect. This suggests that the antiallergic effects of licorice are mainly due to glycyrrhizin, 18beta-glycyrrhetinic acid, and liquiritigenin, which can relieve IgE-induced allergic diseases such as dermatitis and asthma.
Shin
Yong-Wook
YW
College of Pharmacy, Kyung Hee University, Dongdaemun-ku, Seoul, Korea.
Bae
Eun-Ah
EA
Lee
Bomi
B
Lee
Seung Ho
SH
Kim
Jeong Ah
JA
Kim
Yeong-Shik
YS
Kim
Dong-Hyun
DH
D000818:Animals; D018926:Anti-Allergic Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D001249:Asthma / Q000139:chemically induced / Q000517:prevention & control*; D001522:Behavior, Animal / Q000187:drug effects; D006035:Glycyrrhiza; D007073:Immunoglobulin E / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D010047:Ovalbumin; D010323:Passive Cutaneous Anaphylaxis; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D018517:Plant Roots; D003189:p-Methoxy-N-methylphenethylamine
D018926:Anti-Allergic Agents; D010936:Plant Extracts; D007073:Immunoglobulin E; D003189:p-Methoxy-N-methylphenethylamine; D010047:Ovalbumin