title: Transformation of Metarhizium anisopliae with benomyl resistance and green fluorescent protein genes provides a tag for genetically engineered strains.
abstract: A plasmid, pBGFP, carrying green fluorescent protein (gfp) and benomyl-resistance genes was constructed and transformed into Metarhizium anisopliae. The transformants grew normally and GFP fluorescence was detected. No change was found in virulence for the transformants. Fluorescence was detected in hyphae from the haemolymph of the infected locust, and the benomyl-resistance was maintained. Results suggested that the two markers provided a useful tool for screening and monitoring the engineered strains even after infection.
authors.affiliation: Genetic Engineering Research Center, Institute of Bioengineering, Chongqing University, Chongqing, PR China.
authors.forename: Yueqing
authors.initials: Y
authors.lastname: Cao
authors.forename: Guoxiong
authors.initials: G
authors.lastname: Peng
authors.forename: Zhengbo
authors.initials: Z
authors.lastname: He
authors.forename: Zhongkang
authors.initials: Z
authors.lastname: Wang
authors.forename: Youping
authors.initials: Y
authors.lastname: Yin
authors.forename: Yuxian
authors.initials: Y
authors.lastname: Xia
mesh_terms: D000818:Animals; D001542:Benomyl / Q000494:pharmacology*; D015139:Blotting, Southern; D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics; D025141:Drug Resistance, Fungal / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000378:metabolism; D006458:Hemolymph / Q000382:microbiology; D025301:Hyphae / Q000235:genetics / Q000378:metabolism; D048188:Locusta migratoria / Q000382:microbiology; D052981:Metarhizium / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D008957:Models, Genetic; D010957:Plasmids / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D004271:DNA, Fungal; D049452:Green Fluorescent Proteins; D001542:Benomyl