[Evaluation of strand displacement amplification in the diagnosis of tuberculosis].
OBJECTIVE
To study the clinical significance and reliability of strand displacement amplification (SDA) in detecting Mycobacterium tuberculosis complex.


METHODS
SDA and fluorescence quantitative polymerase chain reaction (FQ-PCR) were employed to detect samples from 453 cases of tuberculosis, including 332 sputum samples, 78 samples of pleural effusion, and 43 samples of cerebrospinal fluid.


RESULTS
In the 332 sputum samples, 131 were culture-positive, of which 110 samples (88 smear-positive) were Mycobacterium tuberculosis complex and 21 samples (20 smear-positive) were nontuberculous Mycobacteria. The sensitivity and specificity of SDA for the 110 samples of Mycobacterium tuberculosis complex and 21 samples of nontuberculous Mycobacteria were 99.1%, 95.2% and 94.6%, 95.2%, respectively. The positive rates in the 311 cases of Mycobacterium tuberculosis complex for SDA and FQ-PCR were 55.3% (172/311) and 47.0% (146/311), respectively. There were 20 smear positive samples in the 121 samples of pleural effusion and cerebrospinal fluid, of which 19 were Mycobacterium tuberculosis, 1 nontuberculous Mycobacterium. The positive rates for SDA and FQ-PCR were 43.4% (52/120) and 33.4% (40/120), respectively. Internal amplification control (IAC) was designed for SDA to achieve accuracy of the results.


CONCLUSIONS
The automatic assay system of SDA is a rapid and specific test for detecting Mycobacterium tuberculosis complex.
Tan
Yao-ju
YJ
Institute of Pulmonary Diseases of Guangzhou Chest Hospital, Guangzhou 510095, China.
Li
Hong-yu
HY
Tang
Lin-guo
LG
Chen
Jiang-hua
JH
Ma
Zhi-ming
ZM
Lin
Qing-yu
QY
He
Ju-lian
JL
Chen
Zhi-cheng
ZC
D001431:Bacteriological Techniques / Q000379:methods*; D004269:DNA, Bacterial; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D013183:Sputum / Q000382:microbiology; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology
D004269:DNA, Bacterial