The post-translational phenotype of collagen synthesized by SAOS-2 osteosarcoma cells.
The human osteosarcoma-derived cell line, SAOS-2, exhibits many of the phenotypic characteristics of osteoblasts including the deposition of types I and V collagens in an extracellular matrix. Lesser amounts of collagen XI chains were also detected. The cell layer collagen contains hydroxylysyl pyridinoline cross-links but without the accompanying lysyl pyridinoline typical of human bone collagen. This indicates that the lysine residues at the two helical cross-linking loci are fully hydroxylated. The isoform of lysyl hydroxylase, LH1, known to be required for full hydroxylation at these sites, was shown to be highly expressed by SAOS-2 cells. Our findings provide insight on the mechanism of post-translational overmodification of lysine residues in collagen made by osteosarcoma tumors, and may be relevant for understanding a similar overmodification observed in osteoporotic bone.
Fernandes
Russell J
RJ
Department of Orthopaedics and Sports Medicine, Box 356500, University of Washington, Seattle, WA 98195-6500, USA. rjf@u.washington.edu
Harkey
Michael A
MA
Weis
Maryann
M
Askew
Jennifer W
JW
Eyre
David R
DR
D000595:Amino Acid Sequence; D045744:Cell Line, Tumor; D003094:Collagen / Q000096:biosynthesis* / Q000737:chemistry; D005786:Gene Expression Regulation; D015894:Genome, Human / Q000235:genetics; D006801:Humans; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D012516:Osteosarcoma / Q000378:metabolism*; D010641:Phenotype; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase / Q000235:genetics / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000235:genetics
D012333:RNA, Messenger; D003094:Collagen; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase