A mushroom lectin from ascomycete Cordyceps militaris.
A mushroom lectin has been purified from ascomycete Cordyceps militaris, which is one of the most popular mushrooms in eastern Asia used as a nutraceutical and in traditional Chinese medicine. This lectin, designated CML, exhibited hemagglutination activity in mouse and rat erythrocytes, but not in human ABO erythrocytes. SDS-PAGE of CML revealed a single band with a molecular mass of 31.0 kDa under both nonreducing and reducing conditions that was stained by silver nitrate, and a 31.4 kDa peak in a Superdex-200 HR gel-filtration column. The hemagglutination activity was inhibited by sialoglycoproteins, but not in by mono- or disaccharides, asialoglycoproteins, or de-O-acetylated glycoprotein. The activity was maximal at pH 6.0-9.1 and at temperatures below 50 degrees C. Circular dichroism spectrum analysis revealed that CML comprises 27% alpha-helix, 12% beta-sheets, 29% beta-turns, and 32% random coils. Its binding specificity and secondary structure are similar to those of a fungal lectin from Arthrobotrys oligospora. However, the N-terminal amino acid sequence of CML differs greatly from those of other lectins. CML exhibits mitogenic activity against mouse splenocytes.
Jung
Eui Cha
EC
Physical Pharmacy Laboratory, College of Pharmacy, Chung-Ang University, 221 Huksuk-dong, Dongjak-ku, Seoul 156-756, South Korea.
Kim
Ki Don
KD
Bae
Chan Hyung
CH
Kim
Ju Cheol
JC
Kim
Dae Kyong
DK
Kim
Ha Hyung
HH
D000595:Amino Acid Sequence; D000818:Animals; D001203:Ascomycota / Q000737:chemistry*; D002850:Chromatography, Gel; D002942:Circular Dichroism; D032661:Cordyceps / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D005615:Freezing; D006384:Hemagglutination / Q000187:drug effects*; D006386:Hemagglutination Tests; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008938:Mitosis / Q000187:drug effects; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010084:Oxidation-Reduction; D037121:Plant Lectins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D011485:Protein Binding; D017433:Protein Structure, Secondary; D051381:Rats; D012795:Sialoglycoproteins / Q000494:pharmacology; D016622:Silver Staining; D013154:Spleen / Q000166:cytology / Q000187:drug effects
D037121:Plant Lectins; D012795:Sialoglycoproteins