Effect of L-glutamic acid on the growth and ammonium removal from ammonium solution and natural wastewater by Chlorella vulgaris NTM06.
The main objective of this laboratory scale experiment was to study the effect of l-glutamic acid on the growth in media and removal of ammonium from ammonium solution and natural wastewater by Chlorella vulgaris NTM06. It was observed that higher levels (1.0% and 1.5%) of l-glutamic acid compared to control (0% l-glutamic acid) negatively affected growth of C. vulgaris NTM06 and enhanced removal of ammonium from ammonium solution as well as natural wastewater. After 24h of incubation, 99% of 169.3mg NH(4)(+)-N/l was removed from ammonium solution by 1.5% l-glutamic acid treated C. vulgairs NTM06 cultures; removal in case of control was 70%. In case of natural wastewaters with initial ammonium concentrations of 1550, 775, 310 and 155 mg NH(4)(+)-N/l, removal after 48 h of incubation were 60%, 88%, 61% and 55% respectively. Ammonium removals from ammonium solutions of pH 4.0-8.0 were similar, whereas adsorption of ammonium ions on to the surface of dead C. vulgaris NTM06 cells was around 11%. Compared to dark, cultures incubated under the light showed higher initial removal of ammonium, however, after 24h, differences were not significant. Further research on the role of l-glutamic acid in micro-algal treatment of wastewater and its combination with other approaches such as co-immobilization of micro-algae with other organisms, starvation of micro-algal cells and the use of polymers is recommended.
Khan
Masil
M
Department of Biochemistry and Applied Biosciences, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, 889-2192 Miyazaki-shi, Miyazaki, Japan.
Yoshida
Naoto
N
D001673:Biodegradation, Environmental / Q000187:drug effects; D048408:Chlorella vulgaris / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development* / Q000528:radiation effects; D018698:Glutamic Acid / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration / Q000187:drug effects / Q000528:radiation effects; D008027:Light; D050296:Microbial Viability / Q000187:drug effects / Q000528:radiation effects; D000644:Quaternary Ammonium Compounds / Q000302:isolation & purification*; D012996:Solutions; D014865:Waste Disposal, Fluid
D000644:Quaternary Ammonium Compounds; D012996:Solutions; D018698:Glutamic Acid