Proteomic of lipid rafts in the exocrine pancreas from diet-induced obese rats.
In the present work, we induced obesity in rats with high-energy-starch diet and studied exocrine pancreas response. The zymogen granule (ZG) or purified plasma membrane (PM) from the exocrine pancreas was used for the isolation of the detergent-resistant membranes (DRMs). Based on high content of cholesterol, GM1, the bile salt dependent lipase (BSDL), and GP2 enrichment, the low-density fractions were defined as lipid rafts. Additionally, the rafts vesicles were determined by immunogold labeling with anti BSDL. By combining MALDI-TOF/MS and nano-LC ESI Q-TOF MS/MS proteomic identification we have selected 33 proteins from the lipid rafts which were classified into at least four functional families. Our data suggest that the acinar PM from the diet-induced obesity rats may be organized into lipid rafts, and characterization of rafts proteome can contribute to improve our understanding of food digestion under obesity.
Berkane
Amine Adda
AA
Université Paul Cézanne, FST-Institut Méditerranéen de Recherche en Nutrition IMRN, UMR INRA 1111/PNV UMR 6153 1147, Marseille, France.
Nguyen
Hang Thi Thu
HT
Tranchida
Fabrice
F
Waheed
Abdul A
AA
Deyris
Valérie
V
Tchiakpe
Léopold
L
Fasano
Caroline
C
Nicoletti
Cendrine
C
Desseaux
Véronique
V
Ajandouz
El Hassan
el H
Comeau
Danielle
D
Comeau
Louis
L
Hiol
Abel
A
D000818:Animals; D002462:Cell Membrane / Q000737:chemistry* / Q000648:ultrastructure; D004032:Diet; D008297:Male; D021962:Membrane Microdomains / Q000737:chemistry* / Q000378:metabolism; D009765:Obesity / Q000378:metabolism*; D046790:Pancreas, Exocrine / Q000737:chemistry* / Q000378:metabolism; D011506:Proteins / Q000032:analysis; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley
D011506:Proteins