Fluorimetric and mass spectrometric study of the interaction of beta-cyclodextrin and osthole.
The inclusion complex of beta-cyclodextrin (beta-CD) and osthole was studied by the electrospray ionization mass spectrometry (ESI-MS) and fluorescence spectrometry. From the mass spectrum, the 1:1 stoichiometric inclusion complex of beta-CD and osthole was observed. The tandem mass spectrum was performed. The fluorescence intensity of osthole increased in the present of beta-CD. According to the 1:1 beta-CD-osthole mode, the dissociation constant (KD) was obtained by ESI-MS and fluorescence spectrometry. The KD of beta-CD-osthole inclusion complex is 6.96 x 10(-3) mol L(-1) obtained by mass spectrometry and that is 8.14 x10 (-3) mol L(-1) obtained by fluorescence spectrometry, which is consistent with each other.
Zhang
Huarong
H
College of Chemistry, Jilin University, Changchun, PR China.
Zhang
Hanqi
H
Qu
Chenling
C
Bai
Lifei
L
Ding
Lan
L
D003374:Coumarins / Q000737:chemistry*; D007700:Kinetics; D013050:Spectrometry, Fluorescence; D021241:Spectrometry, Mass, Electrospray Ionization; D047392:beta-Cyclodextrins / Q000737:chemistry*
D003374:Coumarins; D047392:beta-Cyclodextrins; C031215:betadex; C046627:osthol