The potential of cloud point system as a novel two-phase partitioning system for biotransformation.
Although the extractive biotransformation in two-phase partitioning systems have been studied extensively, such as the water-organic solvent two-phase system, the aqueous two-phase system, the reverse micelle system, and the room temperature ionic liquid, etc., this has not yet resulted in a widespread industrial application. Based on the discussion of the main obstacles, an exploitation of a cloud point system, which has already been applied in a separation field known as a cloud point extraction, as a novel two-phase partitioning system for biotransformation, is reviewed by analysis of some topical examples. At the end of the review, the process control and downstream processing in the application of the novel two-phase partitioning system for biotransformation are also briefly discussed.
Wang
Zhilong
Z
School of Pharmacy, Shanghai Jiao Tong University, Shanghai, PR China. zlwang@sjtu.edu.cn
D001709:Biotechnology / Q000379:methods*; D002384:Catalysis; D009161:Mycobacterium / Q000254:growth & development / Q000378:metabolism*; D010399:Penicillin Amidase / Q000378:metabolism*; D004364:Pharmaceutical Preparations / Q000302:isolation & purification* / Q000378:metabolism; D010648:Phenylacetates / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*; D012997:Solvents / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology; D014867:Water / Q000494:pharmacology
D004364:Pharmaceutical Preparations; D010648:Phenylacetates; D012997:Solvents; D013501:Surface-Active Agents; D014867:Water; D010399:Penicillin Amidase; C025136:phenylacetic acid