HKT1;5-like cation transporters linked to Na+ exclusion loci in wheat, Nax2 and Kna1.
Bread wheat (Triticum aestivum) has a greater ability to exclude Na+ from its leaves and is more salt tolerant than durum wheat (Triticum turgidum L. subsp. durum [Desf.]). A novel durum wheat, Line 149, was found to contain a major gene for Na+ exclusion, Nax2, which removes Na+ from the xylem in the roots and leads to a high K+-to-Na+ ratio in the leaves. Nax2 was mapped to the distal region on chromosome 5AL based on linkage to microsatellite markers. The Nax2 locus on 5AL coincides with the locus for a putative Na+ transporter, HKT1;5 (HKT8). The Nax2 region on 5AL is homoeologous to the region on chromosome 4DL containing the major Na+ exclusion locus in bread wheat, Kna1. A gene member of the HKT1;5 family colocates to the deletion bin containing Kna1 on chromosome 4DL. This work provides evidence that Nax2 and Kna1 are strongly associated with HKT1;5 genes.
Byrt
Caitlin S
CS
Commonwealth Scientific and Industrial Research Organization Plant Industry, Canberra, Australian Capital Territory 2601, Australia. caitlin.byrt@csiro.au
Platten
J Damien
JD
Spielmeyer
Wolfgang
W
James
Richard A
RA
Lagudah
Evans S
ES
Dennis
Elizabeth S
ES
Tester
Mark
M
Munns
Rana
R
D000483:Alleles; D001483:Base Sequence; D027682:Cation Transport Proteins / Q000378:metabolism*; D002412:Cations; D017931:DNA Primers; D017343:Genes, Plant; D014644:Genetic Variation; D017136:Ion Transport; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics; D010940:Plant Proteins / Q000378:metabolism*; D011123:Polyploidy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012964:Sodium / Q000378:metabolism*; D027981:Symporters / Q000378:metabolism*; D014908:Triticum / Q000235:genetics / Q000378:metabolism*
D027682:Cation Transport Proteins; D002412:Cations; D017931:DNA Primers; C088864:HKT1 protein, plant; D010940:Plant Proteins; D027981:Symporters; D012964:Sodium