Effect on separation of injecting samples in a solvent different from the mobile phase.
The separation of compounds possessing low solubility in the mobile phase could be improved by applying stronger solvents for dissolving the feed. A model system has been investigated with ethanol-water as the mobile phase, Chirobiotic T as the stationary phase and D,L-threonine as the sample dissolved in pure water. The adsorption isotherms of D,L-threonine were determined as a function of the water content by means of a peak fitting method. Optimal injection conditions of elution chromatography were determined by process simulation. Finally, the optima obtained were verified with experimental data.
Gedicke
Knut
K
Chemical Process Engineering, Otto-von-Guericke-University, Universitätsplatz 2, 39106 Magdeburg, Germany. knut.gedicke@vst.uni-magdeburg.de
Antos
Dorota
D
Seidel-Morgenstern
Andreas
A
D000327:Adsorption; D002853:Chromatography, Liquid; D003198:Computer Simulation; D000431:Ethanol / Q000737:chemistry; D008956:Models, Chemical; D012995:Solubility; D012997:Solvents / Q000737:chemistry*; D013816:Thermodynamics; D013912:Threonine / Q000737:chemistry; D014867:Water / Q000737:chemistry
D012997:Solvents; D014867:Water; D013912:Threonine; D000431:Ethanol