In situ hybridization of termite microbes.
In situ hybridization is one of the most direct and reliable ways to ascertain the origin of the gene from complex mixed cellular systems. This method is essential for studying communities of uncultured microorganism in their natural ecosystem. In this chapter, we introduce our protocols for the in situ hybridization of the messenger RNA of uncultured symbiotic protists of termite hindgut and the ribosomal RNA of the symbiotic bacteria of the protists using nonradioactive labeling protocols. We hope that you will find these protocols useful in your own work to unravel the complex functions and to discover new organisms in the ecosystem.
Moriya
Shigeharu
S
Environmental Molecular Biology Laboratory, RIKEN Institute, Yokohama City, Japan.
Noda
Satoko
S
Ohkuma
Moriya
M
Kudo
Toshiaki
T
D000818:Animals; D001431:Bacteriological Techniques; D001483:Base Sequence; D004064:Digestive System / Q000382:microbiology; D017403:In Situ Hybridization / Q000379:methods*; D020049:Isoptera / Q000382:microbiology*; D008967:Molecular Biology / Q000379:methods; D015336:Molecular Probe Techniques; D015345:Oligonucleotide Probes / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification; D012335:RNA, Ribosomal / Q000235:genetics / Q000302:isolation & purification; D013559:Symbiosis
D015345:Oligonucleotide Probes; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012335:RNA, Ribosomal