title: Subgingival plaque microbiota in HIV positive patients.
abstract: OBJECTIVE
  To describe and compare the predominant bacterial and fungal species associated with gingivitis, periodontitis, and linear gingival erythema (LGE), in HIV positive subjects with different immune status.
  
  
  METHODS
  Viral loads and CD4 levels determined HIV disease status. From pooled subgingival plaque, 16S and 18S rDNA were cloned and sequenced to determine species identity.
  
  
  RESULTS
  One hundred and nine bacterial species were identified from 14 subjects. Nearly half of the species were not cultivable. Notably, the classical putative periodontal pathogens, Treponema denticola, Porphyromonas gingivalis and Tannerella forsythia were below the limit of detection and were not detected. Species of Gemella, Dialister, Streptococcus and Veillonella were predominant. In one HIV positive subject with periodontitis and low viral load, Gemella morbillorum, a known opportunistic pathogen, constituted 84% of the clones. Saccharomyces cerevisiae was the only fungal species detected in an LGE subject and in periodontitis subjects with high viral loads. In periodontitis patients with low viral loads, Candida albicans was predominant, while S. cerevisiae was only a minor component.
  
  
  CONCLUSIONS
  These case studies suggest that other bacterial species, rather than the classical periodontal pathogens, may be involved in periodontal diseases of subjects with HIV. These data are indicative of opportunistic infections in a highly susceptible immunocompromised host.
authors.affiliation: Forsyth Institute, Boston, MA, USA. jornaaas@odont.uio.no
authors.forename: J A
authors.initials: JA
authors.lastname: Aas
authors.forename: S M
authors.initials: SM
authors.lastname: Barbuto
authors.forename: T
authors.initials: T
authors.lastname: Alpagot
authors.forename: I
authors.initials: I
authors.lastname: Olsen
authors.forename: F E
authors.initials: FE
authors.lastname: Dewhirst
authors.forename: B J
authors.initials: BJ
authors.lastname: Paster
mesh_terms: D000328:Adult; D003773:Dental Plaque / Q000382:microbiology*; D005891:Gingivitis / Q000382:microbiology; D006679:HIV Seropositivity / Q000276:immunology / Q000382:microbiology*; D006801:Humans; D008297:Male; D010518:Periodontitis / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D012337:RNA, Ribosomal, 18S / Q000032:analysis; D017422:Sequence Analysis, DNA / Q000379:methods; D019562:Viral Load
chemical_list: D012336:RNA, Ribosomal, 16S; D012337:RNA, Ribosomal, 18S