The development of a bioengineered organ germ method.
To bioengineer ectodermal organs such as teeth and whisker follicles, we developed a three-dimensional organ-germ culture method. The bioengineered tooth germ generated a structurally correct tooth, after both in vitro organ culture as well as transplantation under a tooth cavity in vivo, showing penetration of blood vessels and nerve fibers. Our method provides a substantial advance in the development of bioengineered organ replacement strategies and regenerative therapies.
Nakao
Kazuhisa
K
Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba, 278-8510, Japan.
Morita
Ritsuko
R
Saji
Yasumitsu
Y
Ishida
Kentaro
K
Tomita
Yusuke
Y
Ogawa
Miho
M
Saitoh
Masahiro
M
Tomooka
Yasuhiro
Y
Tsuji
Takashi
T
D000818:Animals; D021522:Bioartificial Organs; D001698:Biomedical Engineering / Q000379:methods; D002454:Cell Differentiation; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D009924:Organ Culture Techniques / Q000379:methods; D038081:Organogenesis / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*; D014070:Tooth / Q000166:cytology*; D014738:Vibrissae / Q000166:cytology*