Alternative medium for production of Pleurotus ostreatus biomass and potential antitumor polysaccharides.
Pleurotus species are recognized for producing beta-glucans with important medicinal properties as a constituent of the cellular wall of the fruiting body or of the mycelium. The aims of this work were to select a culture medium that maximized the production of biomass and polysaccharides produced by Pleurotus ostreatus DSM 1833 and to evaluate the selected medium in two values of initial oxygen transfer rate -K(L)a (10.2 and 19.3 h(-1)). A 2* *4 factorial design was constructed to evaluate the supplementation of wheat extract with corn steep liquor--CSL (10 or 20 g L(-1)), yeast extract--YE (2 or 5gL(-1)), ammonium sulfate--AS (0 or 5 g L(-1)) and glucose (20 or 40 g L(-1)). In terms of maximum productivity in biomass and global productivity in polysaccharides, the best values were obtained when 5 g L(-1) of YE and 40 g L(-1) of glucose were used. In terms of maximum concentration of biomass, the best results were obtained when 20 g L(-1) of CSL and 40 g L(-1) of glucose were used. The best results in terms of production of biomass and polysaccharides were achieved when lower initial K(L)a (10.2 h(-1)) was used.
Gern
Regina Maria Miranda
RM
Department of Biology Sciences, University of the Region of Joinville, UNIVILLE, M Box 246, CEP 89.201-972 Joinville, Santa Catarina, Brazil. rgern@univille.edu.br
Wisbeck
Elisabeth
E
Rampinelli
Jamile Rosa
JR
Ninow
Jorge Luiz
JL
Furlan
Sandra Aparecida
SA
D000970:Antineoplastic Agents / Q000378:metabolism*; D018533:Biomass; D002244:Carbon / Q000737:chemistry; D003470:Culture Media / Q000737:chemistry*; D009584:Nitrogen / Q000737:chemistry; D010100:Oxygen / Q000378:metabolism; D020076:Pleurotus / Q000254:growth & development* / Q000378:metabolism*; D011134:Polysaccharides / Q000096:biosynthesis*
D000970:Antineoplastic Agents; D003470:Culture Media; D011134:Polysaccharides; D002244:Carbon; D009584:Nitrogen; D010100:Oxygen