Detection of transcript for brain-type fatty Acid-binding protein in tumor and urine of patients with renal cell carcinoma.
OBJECTIVE
To clarify the gene expression patterns of fatty acid-binding protein (FABP) and evaluate it as a potential marker for the diagnosis of renal cell carcinoma (RCC). RCC is the most common renal neoplasm.


METHODS
The expression of eight FABP genes in normal human tissues, tumor cell lines, and surgically resected RCC tissues (n = 54) was evaluated by reverse transcriptase-polymerase chain reaction. Additionally, the gene expression of FABPs in the urine of healthy volunteers (n = 12) and patients with RCC (n = 5) was investigated.


RESULTS
In these results, the carcinoma tissues but not the noncancerous (normal) parts of the kidney samples resected from patients with RCC expressed the transcript for brain-type FABP (B-FABP), indicating that expression of the B-FABP gene is a novel marker for RCC. Furthermore, the B-FABP cDNA fragment was not amplified by reverse transcriptase-polymerase chain reaction in the urine samples of healthy donors or patients with RCC after surgical operation. However, B-FABP cDNA was amplified in the patients' urine samples collected before surgery.


CONCLUSIONS
This novel method can be used as a powerful ancillary in the diagnosis of RCC.
Teratani
Takumi
T
Laboratory of Host Defenses, University of Shizuoka Graduate School of Health Science, Suruga-ku, Shizuoka, Japan.
Domoto
Tomohiro
T
Kuriki
Ken
K
Kageyama
Teruyo
T
Takayama
Tatsuya
T
Ishikawa
Akira
A
Ozono
Seiichiro
S
Nozawa
Ryushi
R
D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000097:blood*; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000473:pathology / Q000652:urine; D045744:Cell Line, Tumor; D016009:Chi-Square Distribution; D018076:DNA, Complementary / Q000032:analysis; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics* / Q000473:pathology / Q000652:urine; D008969:Molecular Sequence Data; D011336:Probability; D011379:Prognosis; D012313:RNA / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012494:Sampling Studies; D012680:Sensitivity and Specificity; D046509:Tissue Culture Techniques
D014408:Biomarkers, Tumor; D018076:DNA, Complementary; D050556:Fatty Acid-Binding Proteins; D012313:RNA