Tooth regeneration from newly established cell lines from a molar tooth germ epithelium.
In order to investigate tooth development, several cell lines of the dental epithelium and ectomesenchyme have been established. However, no attempt has been reported to regenerate teeth with cell lines. Here, we have established several clonal cell lines of the dental epithelium from a p53-deficient fetal mouse. They expressed specific markers of the dental epithelium such as ameloblastin and amelogenin. A new method has been developed to bioengineer tooth germs with dental epithelial and mesenchymal cells. Reconstructed tooth germs with cell lines and fetal mesenchymal cells were implanted under kidney capsule. The germs regenerated teeth with well-calcified structures as seen in natural tooth. Germs without the cell lines developed bone. This is the first success to regenerate teeth with dental epithelial cell lines. They are useful models in vitro for investigation of mechanisms in morphogenesis and of cell lineage in differentiation, and for clinical application for tooth regeneration.
Komine
Akihiko
A
Department of Biological Science and Technology, and Tissue Engineering Research Center, Tokyo University of Science, Yamazaki 2641, Noda, Chiba 278-8510, Japan.
Suenaga
Momoko
M
Nakao
Kazuhisa
K
Tsuji
Takashi
T
Tomooka
Yasuhiro
Y
D053523:Amelogenin / Q000032:analysis; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002460:Cell Line; D003746:Dental Enamel Proteins / Q000032:analysis; D004848:Epithelium / Q000502:physiology; D005854:Germ Cells / Q000166:cytology / Q000502:physiology; D051379:Mice; D008817:Mice, Mutant Strains; D008963:Molar / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D012038:Regeneration; D023822:Tissue Engineering; D014070:Tooth / Q000166:cytology / Q000502:physiology; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
C490890:Ambn protein, mouse; D053523:Amelogenin; D015415:Biomarkers; D003746:Dental Enamel Proteins; D016159:Tumor Suppressor Protein p53