Genetic diversity of Enterococcus faecium isolated from Bryndza cheese.
One hundred and seventy-six Enterococcus faecium isolates from Slovak dairy product Bryndza were tested for the presence of plasmid DNA. Eighty-two isolates were positive and their plasmid DNA was isolated and digested by EcoRI and HindIII restriction endonucleases. The patterns obtained were compared with those obtained after pulsed-field gel electrophoresis of macrorestriction fragments (PFGE), (GTG)(5)-PCR and ERIC-PCR. All these molecular approaches were applied for the study of genetic variability and determination of strain relatednesses among plasmid-positive isolates of E. faecium. In general, all methods revealed a considerable genetic diversity of E. faecium isolates. Plasmid profiling and ERIC-PCR have offered a higher resolution than PFGE and (GTG)(5)-PCR.
Jurkovic
D
D
Institute of Cell Biology, Faculty of Natural Sciences, Mlynská dolina, 84215 Bratislava, Slovakia. jurkovicd@stonline.sk
Krizková
L
L
Sojka
M
M
Takácová
M
M
Dusinský
R
R
Krajcovic
J
J
Vandamme
P
P
Vancanneyt
M
M
D002611:Cheese / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis*; D016521:Electrophoresis, Gel, Pulsed-Field; D016984:Enterococcus faecium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D005516:Food Microbiology; D014644:Genetic Variation; D010957:Plasmids; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping
D004269:DNA, Bacterial