Synthesis and recognition of novel isonucleoside triphosphates by DNA polymerases.
Isonucleosides have been attracting a lot of attention in recent years due to the chemical and enzymatic stability and potential anticancer and antiviral activities. We have reported some of the isonucleosides which exhibited significant anticancer activity and found that the oligonucleotide incorporated with isonucleoside could increase the enzymatic stability against the degradation by phosphodiesterase. In this paper, we investigated the recognition of the isonucleoside triphosphates 1-6 by Taq, Vent(exo(-)), DeepVent(exo(-)), 9 degrees Nm, and Therminator DNA polymerases by a non-radioactivity method. We found that most of the isonucleoside triphosphates can be recognized by various DNA polymerase and act as terminators. Isonucleoside triphosphates 2 and 6 can be incorporated as substrates into the primer at 3' terminus to lengthen the chain dependent on a DNA template by Vent(exo(-)) and DeepVent(exo(-)) DNA polymerases.
Jiang
Caiwu
C
State Key Laboratory of Natural and Biomimetic Drug, School of Pharmaceutical Sciences, Peking University, Beijing 100083, PR China.
Li
Bingchao
B
Guan
Zhu
Z
Yang
Zhenjun
Z
Zhang
Liangren
L
Zhang
Lihe
L
D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000494:pharmacology*; D000998:Antiviral Agents / Q000138:chemical synthesis* / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000138:chemical synthesis* / Q000494:pharmacology*; D007202:Indicators and Reagents; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D019384:Nucleic Acid Synthesis Inhibitors; D009705:Nucleosides / Q000378:metabolism; D013237:Stereoisomerism
D000970:Antineoplastic Agents; D000998:Antiviral Agents; D017931:DNA Primers; D004791:Enzyme Inhibitors; D007202:Indicators and Reagents; D019384:Nucleic Acid Synthesis Inhibitors; D009705:Nucleosides; D004247:DNA