Metal particle-enhanced fluorescent immunoassays on metal mirrors.
We present fluoroimmunoassays on plain metal-coated surfaces (metal mirrors) enhanced by metal nanoparticles (silver island films [SIFs]). Metal mirrors (aluminum, gold, or silver protected with a thin silica layer) were coated with SIFs, and an immunoassay (model assay for rabbit immunoglobulin G or myoglobin immunoassay) was performed on this surface using fluorescently labeled antibodies. Our results showed that SIFs alone (on glass surface not coated with metal) enhance the immunoassay signal approximately 3- to 10-fold. Using a metal mirror instead of glass as support for SIFs results in up to 50-fold signal enhancement.
Matveeva
Evgenia G
EG
Department of Molecular Biology and Immunology, Health Science Center, University of North Texas, Fort Worth, TX 76107, USA. ematveev@hsc.unt.edu <ematveev@hsc.unt.edu>
Gryczynski
Ignacy
I
Barnett
Anne
A
Leonenko
Zoya
Z
Lakowicz
Joseph R
JR
Gryczynski
Zygmunt
Z
D000818:Animals; D015200:Fluoroimmunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis; D053768:Metal Nanoparticles / Q000737:chemistry*; D008670:Metals / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008962:Models, Theoretical; D009211:Myoglobin / Q000032:analysis; D036103:Nanotechnology / Q000379:methods; D011817:Rabbits; D015203:Reproducibility of Results; D012834:Silver / Q000737:chemistry
D007074:Immunoglobulin G; D008670:Metals; D009211:Myoglobin; D012834:Silver