The use of enzyme-linked immunosorbent assays (ELISA) for the determination of pollutants in environmental and industrial wastes.
Twelve enzyme-linked immunosorbent assays (ELISA), for the determination of surfactants [linear alkylbenzene sulfonates (LAS), alkyl ethoxylates (AE), and alkylphenol ethoxylates (APE)], endocrine disruptors [alkylphenol (AP), AP + APE, and bisphenol A (BPA)], estrogens [17beta-estradiol (E2), estrone (El), estrogen (ES: El + E2 + estriol (E3)), 1 7alfa-ethynylestradiol (EE2)], dioxins and polychlorinated biphenyls (PCBs), were validated on environmental water and industrial wastes. The lowest quantification limits of these ELISAs were 0.05 microg/L (BPA, E2, El, ES and EE2), 2 microg/L (AE), 3 microg/L (dioxins and PCBs), 5 microg/L (AP, AP + APE) and 20 microg/L (LAS and APE). To apply these ELISAs to environmental or industrial waste samples, simple and appropriate pre-treatment methods were also developed for each ELISA. With optimized pre-treatments, the values of ELISAs were well co-related, in all cases, to those of instrumental analytical methods such as liquid chromatography (HPLC), liquid chromatography-tandem mass spectrometry (LC-MS/MS), and high-resolution gas chromatography mass spectrometry (HR-GC-MS), etc.
Hirobe
M
M
Japan EnviroChemicals, Ltd., 2-17-85 Juso-Honmachi Yodogawa-ku, Osaka 532-0024, Japan. hirobe_masato@jechem.co.jp
Goda
Y
Y
Okayasu
Y
Y
Tomita
J
J
Takigami
H
H
Ike
M
M
Tanaka
H
H
D004147:Dioxins / Q000032:analysis; D004785:Environmental Pollutants / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004967:Estrogens / Q000032:analysis; D005618:Fresh Water; D007220:Industrial Waste / Q000032:analysis*; D011078:Polychlorinated Biphenyls / Q000032:analysis; D012623:Seawater; D014873:Water Pollutants / Q000032:analysis*
D004147:Dioxins; D004785:Environmental Pollutants; D004967:Estrogens; D007220:Industrial Waste; D014873:Water Pollutants; D011078:Polychlorinated Biphenyls