title: A method for Pmo25-associated kinase assay in fission yeast: the activity is dependent on two gC kinases Nak1 and Sid1.
abstract: In fission yeast, the conserved proteins, MO25/Pmo25, GC kinase/Nak1, Furry/Mor2, NDR kinase/Orb6, and Mob2, constitute the morphogenesis Orb6 network (MOR). Previously we showed that Pmo25 functions as an upstream component of MOR and that it plays a connecting role between the septation initiation network (SIN) and MOR. Here we establish a Pmo25-associated kinase assay and show that the activity is dependent on Nak1/MOR and Sid1/SIN.
authors.affiliation: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Kume
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Goshima
authors.forename: Kohji
authors.initials: K
authors.lastname: Miyahara
authors.forename: Takashi
authors.initials: T
authors.lastname: Toda
authors.forename: Dai
authors.initials: D
authors.lastname: Hirata
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D049452:Green Fluorescent Proteins / Q000235:genetics; D004676:Myelin Basic Protein / Q000235:genetics; D011494:Protein Kinases / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases; D012568:Schizosaccharomyces / Q000201:enzymology*; D029702:Schizosaccharomyces pombe Proteins / Q000032:analysis* / Q000378:metabolism*; D014461:Ultracentrifugation
chemical_list: D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D004676:Myelin Basic Protein; C508251:Pmo25 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins; D049452:Green Fluorescent Proteins; D011494:Protein Kinases; C409036:Sid1 kinase; C500976:Nak1 protein, S pombe; D017346:Protein Serine-Threonine Kinases