title: Elevated copy number of L-A virus in yeast mutant strains defective in ribosomal stalk.
abstract: The eukaryotic ribosomal stalk, composed of the P-proteins, is a part of the GTPase-associated-center which is directly responsible for stimulation of translation-factor-dependent GTP hydrolysis. Here we report that yeast mutant strains lacking P1/P2-proteins show high propagation of the yeast L-A virus. Affinity-capture-MS analysis of a protein complex isolated from a yeast mutant strain lacking the P1A/P2B proteins using anti-P0 antibodies showed that the Gag protein, the major coat protein of the L-A capsid, is associated with the ribosomal stalk. Proteomic analysis revealed that the elongation factor eEF1A was also present in the isolated complex. Additionally, yeast strains lacking the P1/P2-proteins are hypersensitive to paromomycin and hygromycin B, underscoring the fact that structural perturbations in the stalk strongly influence the ribosome function, especially at the level of elongation.
authors.affiliation: Department of Molecular Biology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland.
authors.forename: Dawid
authors.initials: D
authors.lastname: Krokowski
authors.forename: Marek
authors.initials: M
authors.lastname: Tchorzewski
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Boguszewska
authors.forename: Adam R
authors.initials: AR
authors.lastname: McKay
authors.forename: Sarah L
authors.initials: SL
authors.lastname: Maslen
authors.forename: Carol V
authors.initials: CV
authors.lastname: Robinson
authors.forename: Nikodem
authors.initials: N
authors.lastname: Grankowski
mesh_terms: D036022:Capsid Proteins / Q000378:metabolism; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006921:Hygromycin B / Q000494:pharmacology; D009154:Mutation; D010303:Paromomycin / Q000494:pharmacology; D020543:Proteome; D012270:Ribosomes; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000821:virology*; D053719:Tandem Mass Spectrometry
chemical_list: D036022:Capsid Proteins; D020543:Proteome; D006921:Hygromycin B; D010303:Paromomycin