Effects of probiotic Lactobacillus paracasei treatment on the host gut tissue metabolic profiles probed via magic-angle-spinning NMR spectroscopy.
We have used a simplified gnotobiotic mouse model to evaluate the effects of single bacterial species, Lactobacillus paracasei NCC2461, on the metabolic profiles of intact intestinal tissues using high-resolution magic-angle-spinning 1H NMR spectroscopy (HRMAS). A total of 24 female gnotobiotic mice were divided into three groups: a control group supplemented with water and two groups supplemented with either live L. paracasei or a gamma-irradiated equivalent. HRMAS was used to characterize the biochemical components of intact epithelial tissues from the duodenum, jejunum, ileum, proximal, and distal colons in all animals and data were analyzed using chemometrics. Variations in relative concentrations of amino acids, anti-oxidant, and creatine were observed relating to different physiological properties in each intestinal tissue. Metabolic characteristics of lipogenesis and fat storage were observed in the jejunum and colon. Colonization with live L. paracasei induced region-dependent changes in the metabolic profiles of all intestinal tissues, except for the colon, consistent with modulation of intestinal digestion, absorption of nutrients, energy metabolism, lipid synthesis and protective functions. Ingestion of gamma-irradiated bacteria produced no effects on the observed metabolic profiles. 1H MAS NMR spectroscopy was able to generate characteristic metabolic signatures reflecting the structure and function of intestinal tissues. These signals acted as reference profiles with which to compare changes in response to gut microbiota manipulation at the tissue level as demonstrated by ingestion of a bacterial probiotic.
Martin
Francois-Pierre J
FP
Department of Biomolecular Medicine, Division of Surgery, Oncology, Reproductive Biology and Anaesthetics, Faculty of Medicine, Imperial College London, South Kensington, London SW7 2AZ, UK.
Wang
Yulan
Y
Sprenger
Norbert
N
Holmes
Elaine
E
Lindon
John C
JC
Kochhar
Sunil
S
Nicholson
Jeremy K
JK
D000596:Amino Acids / Q000032:analysis / Q000378:metabolism; D000818:Animals; D004734:Energy Metabolism; D005260:Female; D005856:Germ-Free Life; D007249:Inflammation / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000187:drug effects* / Q000382:microbiology; D007778:Lactobacillus / Q000528:radiation effects; D008055:Lipids / Q000032:analysis / Q000096:biosynthesis; D051379:Mice; D008815:Mice, Inbred Strains; D009119:Muscle Contraction; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D019936:Probiotics / Q000494:pharmacology* / Q000528:radiation effects
D000596:Amino Acids; D008055:Lipids