Single multiplex assay to identify simultaneously enteropathogenic, enteroaggregative, enterotoxigenic, enteroinvasive and Shiga toxin-producing Escherichia coli strains in Brazilian children.
A multiplex PCR to differentiate typical and atypical enteropathogenic Escherichia coli (EPEC), enteroaggregative E. coli (EAEC), enterotoxigenic (ETEC), enteroinvasive E. coli (EIEC) and Shiga toxin-producing E. coli (STEC) strains was developed and evaluated. The targets selected for each group were eae and bfpA for EPEC, aggR for EAEC, elt and est for ETEC, ipaH for EIEC and stx for STEC isolates. This PCR was specific and sensitive for rapid detection of target isolates in stools. Among 79 children with acute diarrhea, this technique identified 13 (16.4%) with atypical EPEC, four (5%) with EAEC, three (3.8%) with typical EPEC, one (1.3%) with ETEC and one (1.3%) with EIEC.
Aranda
Katia R S
KR
Departamento de Microbiologia, Imunologia e Parasitologia, São Paulo, SP, Brazil.
Fabbricotti
Sandra H
SH
Fagundes-Neto
Ulysses
U
Scaletsky
Isabel C A
IC
D015373:Bacterial Typing Techniques; D001938:Brazil; D002675:Child, Preschool; D003967:Diarrhea / Q000382:microbiology; D004926:Escherichia coli / Q000145:classification* / Q000302:isolation & purification / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D022621:Shiga Toxin / Q000378:metabolism
D022621:Shiga Toxin