title: Immunohistochemical evaluation of substance P and calcitonin gene-related peptide in skin and periosteum after extracorporeal shock wave therapy and radial pressure wave therapy in sheep.
abstract: OBJECTIVE
  To evaluate the effect of focused extracorporeal shock wave therapy (ESWT) and radial pressure wave therapy (RPWT) on immunohistochemical staining for substance P and calcitonin gene-related peptide (CGRP) in the skin and periosteum of sheep.
  
  
  METHODS
  36 sheep.
  
  
  METHODS
  All 4 limbs of 36 sheep were treated with ESWT, RPWT, or a sham treatment. For 14 days after treatment, at least 2 sheep were euthanized daily and tissue was harvested for histologic evaluation of nerves via staining for substance P and CGRP in the skin and periosteum.
  
  
  RESULTS
  No effects of ESWT or RPWT were observed on the number of nerves with stain uptake for substance P or CGRP in the skin or periosteum.
  
  
  CONCLUSIONS
  Substance P- and CGRP-containing nerve fibers are not disrupted by EWST or RPWT. Further studies are needed to identify the mechanism of analgesia observed in association with these treatment modalities.
authors.affiliation: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.
authors.forename: Janan M
authors.initials: JM
authors.lastname: Abed
authors.forename: Scott R
authors.initials: SR
authors.lastname: McClure
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Yaeger
authors.forename: Richard B
authors.initials: RB
authors.lastname: Evans
mesh_terms: D000698:Analgesia / Q000379:methods / Q000662:veterinary; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis* / Q000378:metabolism; D019220:High-Energy Shock Waves / Q000009:adverse effects* / Q000627:therapeutic use*; D007150:Immunohistochemistry / Q000662:veterinary; D009474:Neurons / Q000378:metabolism; D010521:Periosteum / Q000378:metabolism*; D011312:Pressure; D012756:Sheep / Q000502:physiology*; D012867:Skin / Q000378:metabolism*; D013373:Substance P / Q000032:analysis* / Q000378:metabolism; D013997:Time Factors
chemical_list: D013373:Substance P; D015740:Calcitonin Gene-Related Peptide