title: The development of a bioengineered organ germ method.
abstract: To bioengineer ectodermal organs such as teeth and whisker follicles, we developed a three-dimensional organ-germ culture method. The bioengineered tooth germ generated a structurally correct tooth, after both in vitro organ culture as well as transplantation under a tooth cavity in vivo, showing penetration of blood vessels and nerve fibers. Our method provides a substantial advance in the development of bioengineered organ replacement strategies and regenerative therapies.
authors.affiliation: Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, Noda, Chiba, 278-8510, Japan.
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Nakao
authors.forename: Ritsuko
authors.initials: R
authors.lastname: Morita
authors.forename: Yasumitsu
authors.initials: Y
authors.lastname: Saji
authors.forename: Kentaro
authors.initials: K
authors.lastname: Ishida
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Tomita
authors.forename: Miho
authors.initials: M
authors.lastname: Ogawa
authors.forename: Masahiro
authors.initials: M
authors.lastname: Saitoh
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Tomooka
authors.forename: Takashi
authors.initials: T
authors.lastname: Tsuji
mesh_terms: D000818:Animals; D021522:Bioartificial Organs; D001698:Biomedical Engineering / Q000379:methods; D002454:Cell Differentiation; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D009924:Organ Culture Techniques / Q000379:methods; D038081:Organogenesis / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*; D014070:Tooth / Q000166:cytology*; D014738:Vibrissae / Q000166:cytology*