Allicin-induced suppression of Mycobacterium tuberculosis 85B mRNA in human monocytes.
Despite of encountering a robust immune response, Mycobacterium tuberculosis (MTB) successfully survives and persists in the human host. We investigated the early regulation of MTB 85B gene by allicin in MTB-infected human monocytes. During the first 24h of infection, levels of both MTB 85B intracellular mRNA and secreted protein were significantly down-regulated by allicin in a dose-dependent manner, which was mediated by inhibition of glutathione and NF-kappaB pathway. Allicin-induced MTB 85B suppression correlated with suppression of TNF-alpha released from infected monocytes. The allicin-induced up-regulation of glutathione and IFN-gamma with simultaneous decrease in TNF-alpha supports the anti-inflammatory property of allicin by elicitation of protective immune response. Thus, allicin may prove to be valuable in the containment of MTB and therefore be useful as an adjunct in treatment of tuberculosis.
Hasan
Nazarul
N
Department of Biochemistry, Faculty of Medicine, J.N. Medical College, A.M.U., Aligarh 202002, U.P., India.
Siddiqui
Mashiat Ullah
MU
Toossi
Zahra
Z
Khan
Saba
S
Iqbal
Jawed
J
Islam
Najmul
N
D001483:Base Sequence; D017931:DNA Primers; D004220:Disulfides; D004305:Dose-Response Relationship, Drug; D005798:Genes, Bacterial; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D009000:Monocytes / Q000378:metabolism / Q000382:microbiology*; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D012329:RNA, Bacterial / Q000187:drug effects* / Q000235:genetics; D012333:RNA, Messenger / Q000187:drug effects* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013441:Sulfinic Acids / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D017931:DNA Primers; D004220:Disulfides; D012329:RNA, Bacterial; D012333:RNA, Messenger; D013441:Sulfinic Acids; D014409:Tumor Necrosis Factor-alpha; C006452:allicin; D007371:Interferon-gamma; D005978:Glutathione