[Study on continued degradation of BaP and its metabolites].
Two metabolites, cis-BP4, 5-dihydrodiol and cis-BP7, 8-dihydrodiol, were identified by high-performance liquid chromatography (HPLC) during the degradation of BaP by Bacillus-07 (BA-07). The two metabolites were hardly further metabolized for their toxicity to microorganism. To promote degradation of BaP and decrease accumulation of cis-BP4, 5-dihydrodiol and cis-BP7, 8-dihydrodiol, two methods (degradation only by BA-07, degradation by coupling the BA-07 and KMnO4) were compared. In addition, parameters of continued degradation of BaP and the two metabolites were optimized under the experiment conditions. The results showed that (1)the method of coupling the chemical oxidation and biodegradation (BA-07 and KMnO4) was better than only biodegradation (BA-07); (2) residue rate of cis-BP4, 5-dihydrodiol was higher than that of cis-BP7, 8-dihydrodiol when the samples were determined in the same time; (3)the effect of continued degradation was the best when the initial concentration of BaP was 40 microg/mL, pH value of the culture was 7.0, co-metabolic substrates was sodium succinate. Meanwhile, it was put forward that the method of coupling the chemical oxidation and biodegradation was effective on continued degradation of persistent organic contaminants in the environment.
Zang
Shu-yan
SY
Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China. zangshuyan@126.com
Li
Pei-Jun
PJ
Zhou
Qi-xing
QX
Wang
Xin
X
Lin
Gui-feng
GF
Wang
Juan
J
D001407:Bacillus / Q000254:growth & development / Q000378:metabolism*; D001564:Benzo(a)pyrene / Q000737:chemistry / Q000378:metabolism*; D001673:Biodegradation, Environmental; D004101:Dihydroxydihydrobenzopyrenes / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D012989:Soil Pollutants / Q000737:chemistry / Q000378:metabolism*
D004101:Dihydroxydihydrobenzopyrenes; D012989:Soil Pollutants; C017228:benzo(a)pyrene 7,8-dihydrodiol; C015813:4,5-dihydroxy-4,5-dihydrobenzo(a)pyrene; D001564:Benzo(a)pyrene