Identification and characterization of a novel acidotolerant Fe(III)-reducing bacterium from a 3,000-year-old acidic rock drainage site.
Acidic, ochre-precipitating springs at Mam Tor, East Midlands, UK, are analogous to sites impacted by acid mine drainage over prolonged periods of time, and were studied for the presence of Fe(III)-reducing bacteria. From enrichment cultures inoculated with Mam Tor sediment, a facultative anaerobe capable of reducing Fe(III) at pH values as low as three was isolated. 16S rRNA gene analysis showed that this bacterium is a close relative of Serratia species and not previously shown to respire using Fe(III) as an electron acceptor. Direct cell counts of the isolate grown with Fe(III)-NTA coupled with protein assays suggest that this bacterium is able to conserve energy for growth through Fe(III) reduction.
Adams
Laura K
LK
Williamson Research Centre for Molecular Environmental Science and The School of Earth, Atmospheric and Environmental Sciences, University of Manchester, Manchester, UK.
Boothman
Christopher
C
Lloyd
Jonathan R
JR
D000143:Acids; D005290:Ferric Compounds / Q000378:metabolism*; D019015:Geologic Sediments / Q000382:microbiology*; D010084:Oxidation-Reduction; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012705:Serratia / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology; D013045:Species Specificity; D006113:United Kingdom
D000143:Acids; D005290:Ferric Compounds; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S