Mass spectrometric analysis of 4-hydroxy-1-(3-pyridyl)-1-butanone-releasing DNA adducts in human lung.
An improved analytical method was developed for the analysis of 4-hydroxy-1-(3-pyridyl)-1-butanone (HPB)-releasing DNA adducts in lung samples of patients undergoing surgery for lung cancer. HPB-releasing adducts can be formed by metabolic activation of the tobacco-specific nitrosamines 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone and N'-nitrosonornicotine, and have been reported to play an important role in tobacco carcinogenesis. [2,2,3,3-D(4)]HPB (D(4)-HPB) was used as an internal standard, and HPB released by acid hydrolysis of DNA was determined by gas chromatography/mass spectrometry in the negative ion chemical ionisation mode. The method is sensitive with a limit of detection of 5.9 fmol HPB and a limit of quantification of 15.2 fmol HBP/mg DNA. The recovery of HPB was 82+/-17% and the background response was 10.1+/-1.8 fmol HPB/sample. The concentration of HPB-releasing lung DNA adducts was significantly higher (p<0.0001) in 21 self-reported smokers compared to in 11 self-reported nonsmokers (404+/-258 fmol versus 59+/-56 fmol HPB/mg DNA, respectively). HPB-releasing hemoglobin adduct concentrations were only marginally higher in a subset of 12 smokers compared to in 7 nonsmokers (63+/-53 fmol versus 42+/-34 fmol HPB/g hemoglobin; p=0.36). No correlation was found between HPB-releasing adducts in DNA and hemoglobin (p=0.074).
Hölzle
Daniel
D
Walther Straub Institute of Pharmacology and Toxicology, Ludwig-Maximilians University, Goethestrasse 33, D-80336 Munich, Germany.
Schlöbe
Daniela
D
Tricker
Anthony R
AR
Richter
Elmar
E
D000230:Adenocarcinoma / Q000737:chemistry / Q000209:etiology / Q000378:metabolism; D000368:Aged; D000369:Aged, 80 and over; D002074:Butanones / Q000032:analysis* / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000378:metabolism; D018736:DNA Adducts / Q000032:analysis* / Q000378:metabolism; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D008168:Lung / Q000737:chemistry* / Q000378:metabolism; D008175:Lung Neoplasms / Q000737:chemistry / Q000209:etiology / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011725:Pyridines / Q000032:analysis* / Q000378:metabolism; D012680:Sensitivity and Specificity; D012907:Smoking / Q000378:metabolism
D002074:Butanones; D018736:DNA Adducts; D006454:Hemoglobins; D011725:Pyridines; C482281:4-hydroxy-1-(3-pyridyl)-1-butanone