Genetic structure of Tuber mesentericum Vitt. based on polymorphisms at the ribosomal DNA ITS.
Tuber mesentericum fruit bodies are in increasing demand on the food market and are an important economic resource for southern Italy, their major production area. Because molecular studies on this truffle species are very scarce, we analyzed ITS1 and ITS2 nucleotide variability of 126 ascocarps of T. mesentericum collected in different European areas, mainly southern Italy. The results of haplotype distribution, analysis of molecular variance, and spatial analysis of molecular variance analyses show strong genetic structuring of the samples collected in the different geographic areas, confirmed by parsimony and distance analyses. In particular, the Italian samples seem to be a well-distinguished group.
Sica
Maria
M
Department of Biological Sciences, Section of Genetics and Molecular Biology, University of Naples Federico II, via Mezzocannone 8, 80134, Napoli, Italy.
Gaudio
Luciano
L
Department of Biological Sciences, Section of Genetics and Molecular Biology, University of Naples Federico II, via Mezzocannone 8, 80134, Napoli, Italy.
Aceto
Serena
S
Department of Biological Sciences, Section of Genetics and Molecular Biology, University of Naples Federico II, via Mezzocannone 8, 80134, Napoli, Italy. serena.aceto@unina.it.
D001203:Ascomycota / Q000145:classification* / Q000235:genetics*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics*; D004783:Environmental Microbiology; D005060:Europe; D048690:Fruiting Bodies, Fungal / Q000235:genetics*; D006239:Haplotypes; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer