title: Phenotypes, serotypes and antibiotic susceptibility of Swedish Porphyromonas gingivalis isolates from periodontitis and periodontal abscesses.
abstract: This study was conducted to reveal phenotypic, serological subtypes and antibiotic susceptibility among fresh isolates of Porphyromonas gingivalis in a Swedish population with periodontitis and periodontal abscess. Fifty-five subgingival strains were isolated and tentatively designated as P. gingivalis from 55 consecutive paper-point samples taken from 51 patients with periodontitis (at least one site with >6-mm pocket depth) in Sweden and were sent in for microbiological evaluation. Eight P. gingivalis strains from periodontal abscesses were also included. Four P. gingivalis strains served as reference and another four type strains were included. The strains were characterized by colony morphology, biochemical tests, enzyme profile, gas-liquid chromatography and antibiotic susceptibility. The strains were further characterized for whole cell protein profiles using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and were identified to serotype by specific monoclonal antibodies. Among the 55 P. gingivalis strains 35 had smooth (S), 13 rough (R) and seven semi-rough colony morphologies. All strains were phenotypically homogeneous in biochemical tests, enzyme profile and antibiotic susceptibility. All strains produced phenylacetic acid and alpha-fucosidase. Almost all (96%) of the subgingival strains, but relatively fewer (62%) of the abscess strains, belonged to serotype A. Two subgingival and three abscess strains were classified as serotype B. No specific SDS-PAGE protein profiles were recorded for the two serotypes. The P. gingivalis strains from Swedish periodontitis cases showed homogeneity in terms of biochemical phenotypes and antibiotic susceptibility patterns. The strains fell into two serotypes, of which serotype A predominated in the periodontitis cases and serotype B was overrepresented in periodontal abscesses.
authors.affiliation: Department of Oral Microbiology, Faculty of Odontology, Sahlgrenska Academy at Göteborg University, Göteborg, Sweden. dahlen@odontologi.gu.se
authors.forename: G
authors.initials: G
authors.lastname: Dahlén
authors.forename: R
authors.initials: R
authors.lastname: Gmür
authors.forename: T
authors.initials: T
authors.lastname: Yoshino
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000032:analysis; D006801:Humans; D008826:Microbial Sensitivity Tests; D010508:Periodontal Abscess / Q000382:microbiology*; D010518:Periodontitis / Q000382:microbiology*; D010641:Phenotype; D016966:Porphyromonas gingivalis / Q000145:classification* / Q000187:drug effects* / Q000201:enzymology; D012703:Serotyping; D013548:Sweden
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins