title: Calcifying epithelial odontogenic tumor: an immunohistochemical case study.
abstract: Calcifying epithelial odontogenic tumor (CEOT) is a rare benign odontogenic tumor. A case of CEOT in a 25-year-old female is presented here. Histologically, the case showed sheets of polyhedral epithelial cells with deep eosinophilic cytoplasm and prominent nuclei. Nuclear pleomorphism and hyperchromatism were evident. Globules of amyloid-like material among the tumor cells were prominent. Also found was a small area demonstrating a cribriform pattern. Immunohistochemical studies with antibodies against basement membrane proteins (laminins 1 and 5, collagen type IV and fibronectin), pan-cytokeratins AE1/AE3, vimentin, S-100 protein and CD 1a were performed. Tumor cells expressed laminins 1 and 5, fibronectin, cytokeratins and vimentin. The amyloid-like material was not reactive to all antibodies examined. A number of dendritic cells among sheets of tumor cells were revealed with strong staining for S-100 protein and CD 1a. These dendritic cells are likely to be Langerhans cells. Hence, immunohistochemistry is a useful method to study the variant of CEOT.
authors.affiliation: Department of Oral Pathology, Faculty of Dentistry, Mahidol University, Yothi Street, Bangkok 10400, Thailand. spoomsawat@yahoo.com
authors.forename: Sopee
authors.initials: S
authors.lastname: Poomsawat
authors.forename: Jirapa
authors.initials: J
authors.lastname: Punyasingh
mesh_terms: D000328:Adult; D002467:Cell Nucleus / Q000378:metabolism / Q000473:pathology; D003593:Cytoplasm / Q000378:metabolism / Q000473:pathology; D003713:Dendritic Cells / Q000378:metabolism / Q000473:pathology; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008339:Mandibular Neoplasms / Q000378:metabolism* / Q000473:pathology*; D009363:Neoplasm Proteins / Q000378:metabolism*; D018333:Odontogenic Cyst, Calcifying / Q000378:metabolism* / Q000473:pathology*
chemical_list: D009363:Neoplasm Proteins