title: Endothelial cells derived from human embryonic stem cells form durable blood vessels in vivo.
abstract: We describe the differentiation of human embryonic stem (hES) cells into endothelial cells using a scalable two-dimensional method that avoids an embryoid-body intermediate. After transplantation into severe combined immunodeficient (SCID) mice, the differentiated cells contributed to arborized blood vessels that integrated into the host circulatory system and served as blood conduits for 150 d.
authors.affiliation: Maine Medical Center Research Institute, Scarborough, Maine 04074, USA.
authors.forename: Zack Z
authors.initials: ZZ
authors.lastname: Wang
authors.forename: Patrick
authors.initials: P
authors.lastname: Au
authors.forename: Tong
authors.initials: T
authors.lastname: Chen
authors.forename: Ying
authors.initials: Y
authors.lastname: Shao
authors.forename: Laurence M
authors.initials: LM
authors.lastname: Daheron
authors.forename: Hao
authors.initials: H
authors.lastname: Bai
authors.forename: Melanie
authors.initials: M
authors.lastname: Arzigian
authors.forename: Dai
authors.initials: D
authors.lastname: Fukumura
authors.forename: Rakesh K
authors.initials: RK
authors.lastname: Jain
authors.forename: David T
authors.initials: DT
authors.lastname: Scadden
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000166:cytology* / Q000254:growth & development*; D002454:Cell Differentiation; D053595:Embryonic Stem Cells / Q000166:cytology*; D042783:Endothelial Cells / Q000166:cytology* / Q000502:physiology; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D014741:Video Recording