Study on the noncovalent complexes of ginsenoside and cytochrome c by electrospray ionization mass spectrometry.
The noncovalent complexes of cytochrome c and ginsenoside were studied by electrospray ionization mass spectrometry (ESI-MS). Ginsenoside Rb2 and Re were bound to cytochrome c to form several complexes with different stoichiometric relation. The 1:1 and 1:2 complexes of cytochrome c to ginsenoside were considered and the dissociation constants were obtained according to the intensities of cytochrome c and complexes when the concentrations of cytochrome c and ginsenoside have been known. Competition experiment was performed to validate the result. The K(D) values obtained with different reactive systems were consistent with each other.
Zhang
Huarong
H
College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012, PR China. analchem@mail.jlu.edu.cn
Ding
Lan
L
Qu
Chenling
C
Li
Dan
D
Zhang
Hanqi
H
D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D045304:Cytochromes c / Q000737:chemistry*; D036145:Ginsenosides / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
D036145:Ginsenosides; D046911:Macromolecular Substances; C048026:ginsenoside Rb2; C049864:ginsenoside Re; D045304:Cytochromes c