title: Estimation of the human daily requirement of vitamin E by turnover kinetics of labeled RRR-alpha-tocopherol.
abstract: The human daily requirement of alpha-tocopherol was estimated by a determination of the turnover kinetics of deuterium-labeled RRR-alpha-tocopherol in two women and three men. The amount of alpha-tocopherol absorbed varied in proportion to the amount of fat eaten with the vitamin, increasing by 0.43 mg for each gram of fat consumed. A fat-free method for administering alpha-tocopherol was developed by vacuum impregnation of apples with the vitamin. Even in the absence of fat, about 10% of the administered alpha-tocopherol was absorbed. The daily requirement of alpha-tocopherol when consumed with a diet containing 21% fat was estimated to be 15 +/- 2 mg, an amount close to the current Estimated Average Requirement (EAR) of 12 mg/d. The study showed that vacuum-impregnated fruit might be a useful way to supplement vitamin E in people eating a low-fat diet.
authors.affiliation: Department of Nutritional Sciences and Toxicology, University of California, Berkeley, California, USA.
authors.forename: George
authors.initials: G
authors.lastname: Wolf
mesh_terms: D019540:Area Under Curve; D001682:Biological Availability; D003903:Deuterium; D005260:Female; D006801:Humans; D008297:Male; D009751:Nutritional Requirements; D014810:Vitamin E / Q000493:pharmacokinetics*; D014815:Vitamins / Q000493:pharmacokinetics*; D024502:alpha-Tocopherol / Q000493:pharmacokinetics
chemical_list: D014815:Vitamins; D014810:Vitamin E; D003903:Deuterium; D024502:alpha-Tocopherol