Micro-determination of nucleic acids with a simple probe manganese chloride based on the fine enhanced resonance light scattering.
Manganese chloride can form large particles with nucleic acids by electrostatic forces, which results in strong enhancement of resonance light scattering (RLS) signals. Based on this phenomenon, a novel and very simple assay of DNA was established. The work conditions have been investigated including the concentration of probe, the acidity of solution, the effect of ionic strength and the selectivity. In acidic solution, the enhanced RLS intensity at 389.5 nm was proportional to the concentration of nucleic acids in the range 0.05-10.0 microg ml(-1) for both ctDNA and fsDNA and 1.0-10.0 microg ml(-1) for yRNA. The limits of detection (LOD, 3sigma) were 0.17, 0.13 and 0.53 ng ml(-1) for ctDNA, fsDNA and yRNA, respectively. Synthetic samples were determined satisfactorily.
Chen
Zhanguang
Z
Department of Chemistry, Shantou University, Shantou 515063, China. kqlu@stu.edu.cn
Liao
Xuhong
X
Zhu
Li
L
Liu
Jinbin
J
Han
Yali
Y
D000818:Animals; D002417:Cattle; D002712:Chlorides / Q000737:chemistry; D004247:DNA / Q000032:analysis; D005399:Fishes; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008027:Light; D008297:Male; D017895:Manganese Compounds / Q000737:chemistry; D008832:Microchemistry / Q000379:methods*; D009691:Nucleic Acid Denaturation; D009696:Nucleic Acids / Q000032:analysis*; D009994:Osmolar Concentration; D012331:RNA, Fungal / Q000032:analysis; D012542:Scattering, Radiation; D012996:Solutions; D055672:Static Electricity
D002712:Chlorides; D017895:Manganese Compounds; D009696:Nucleic Acids; D012331:RNA, Fungal; D012996:Solutions; D004247:DNA; C025340:manganese chloride