Recognition of sugars on surface-bound cap-shaped gold particles modified with a polymer brush.
A dithiolated random copolymer with pendent phenylboronic acid residues (Cys-Poly(3-acrylamidophenylboronic acid-co-2-dimethylaminopropyl methacrylamide), Cys-Poly(APBA-co-DMAPMA)) obtained by photo-iniferter method was accumulated as a polymer brush on a cap-shaped gold particles deposited on a vacuum-evaporated gold film, and the usefulness of the polymer brush as a sensing element for glycoprotein, ovalbumin (OVA), was examined by using UV-vis spectroscopy with a help of surface plasmon resonance. A similar system was constructed with a dithiolated mannose-carrying polymer, dithiolated-poly(2-methacryloyloxyethyl-D-mannopyranoside) (DT-PMEMan), prepared by the atom transfer radical polymerization (ATRP). The brush composed of this polymer was examined as a sensing element for lectin, concanavalin A (Con A). The sensor cells modified with Cys-Poly(APBA-co-DMAPMA) and DT-PMEMan showed a concentration-dependent binding of OVA and Con A, respectively, with a comparable detection limit to those with a monolayer of polymer brush-coated gold particle deposited on a glass substrate. Using this system, it can be expected to open a new perspective to various functional polymer brushes fixed to the cap-shaped gold particle on a solid substrate.
Anraku
Yasutaka
Y
Department of Applied Chemistry, Graduate School of Science and Engineering, University of Toyama, Toyama 930-8555, Japan.
Takahashi
Yoshiko
Y
Kitano
Hiromi
H
Hakari
Mami
M
D000178:Acrylamides / Q000737:chemistry; D000327:Adsorption; D015374:Biosensing Techniques; D001897:Boronic Acids / Q000737:chemistry; D002118:Calcium / Q000737:chemistry; D002241:Carbohydrates / Q000737:chemistry*; D003208:Concanavalin A / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry; D008345:Manganese / Q000737:chemistry; D008863:Microspheres; D010047:Ovalbumin / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D020349:Surface Plasmon Resonance; D013499:Surface Properties
D000178:Acrylamides; D001897:Boronic Acids; D002241:Carbohydrates; D037102:Lectins; D011108:Polymers; D003208:Concanavalin A; D008345:Manganese; D006046:Gold; D010047:Ovalbumin; C099046:N,N-dimethylacrylamide; C010686:benzeneboronic acid; D002118:Calcium