Metabolic analysis of the cinnamate/monolignol pathway in Carthamus tinctorius seeds by a stable-isotope-dilution method.
The present study established a system for comprehensive metabolic analysis of the cinnamate/monolignol and lignan pathways by the use of a stable-isotope-dilution method. The system was successfully applied to characterization of the pathways in Carthamus tinctorius cv. Round-leaved White maturing seeds in combination with administration of stable-isotope-labelled precursors. Experimental results obtained using this technique strongly suggested the intermediacy of ferulic acid in lignan biosynthesis in the plant.
Sakakibara
Norikazu
N
Research Institute for Sustainable Humanosphare, Kyoto University, Uji, Kyoto 611-0011, Japan.
Nakatsubo
Tomoyuki
T
Suzuki
Shiro
S
Shibata
Daisuke
D
Shimada
Mikio
M
Umezawa
Toshiaki
T
D002247:Carbon Isotopes; D031187:Carthamus tinctorius / Q000378:metabolism*; D002934:Cinnamates / Q000737:chemistry / Q000378:metabolism*; D003903:Deuterium; D008401:Gas Chromatography-Mass Spectrometry; D007201:Indicator Dilution Techniques; D017705:Lignans / Q000096:biosynthesis* / Q000737:chemistry; D008031:Lignin / Q000096:biosynthesis* / Q000737:chemistry; D015394:Molecular Structure; D012639:Seeds / Q000378:metabolism
D002247:Carbon Isotopes; D002934:Cinnamates; D017705:Lignans; D008031:Lignin; D003903:Deuterium