Layer-by-layer films from hyaluronan and amine-modified hyaluronan.
Hyaluronan is a polysaccharide that is increasingly investigated for its role in cellular adhesion and for the preparation of biomimetic matrices for tissue engineering. Hyaluronan gels are prepared for application as space fillers, whereas hyaluronan films are usually obtained by adsorbing or grafting a single hyaluronan layer onto a biomaterial surface. Here, we examine the possibility to employ the layer-by-layer technique to deposit thin films of cationic-modified hyaluronan (HA+) and hyaluronan (HA) of controlled thicknesses. The buildup conditions are investigated, and growth is compared to that of other polyelectrolyte multilayer films containing either HA as the polyanion or HA+ as the polycation. The films could be formed in a low ionic strength medium but are required to be cross-linked prior to contact with a physiological medium. NIH3T3 fibroblasts were perfectly viable on self-assembled hyaluronan films with, however, a preference for hyaluronan ending films. These findings point out the possibility to tune the thickness of thin hyaluronan films at the nanometer scale. Such architectures could be employed for investigating cell/substrate interactions or for functionalizing biomaterial surfaces.
Schneider
Aurore
A
Institut National de la Santé et de la Recherche Médicale, Unité 595, 11 rue Humann, 67085 Strasbourg Cedex, France.
Picart
Catherine
C
Senger
Bernard
B
Schaaf
Pierre
P
Voegel
Jean-Claude
JC
Frisch
Benoit
B
D000588:Amines / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials; D002470:Cell Survival; D003432:Cross-Linking Reagents / Q000494:pharmacology; D006820:Hyaluronic Acid / Q000737:chemistry*; D051379:Mice; D018625:Microscopy, Atomic Force; D008956:Models, Chemical; D041681:NIH 3T3 Cells; D011134:Polysaccharides / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013499:Surface Properties; D023822:Tissue Engineering
D000588:Amines; D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D011134:Polysaccharides; D006820:Hyaluronic Acid