title: Dynamics and genetics of PrPSc placental accumulation in sheep.
abstract: Placentae from scrapie-affected ewes are an important source of contamination. This study confirmed that scrapie-incubating ewes bearing susceptible genotypes could produce both abnormal prion protein (PrPSc)-positive and -negative placentae, depending only on the PRP genotype of the fetus. The results also provided evidence indicating that scrapie-incubating ARR/VRQ ewes may be unable to accumulate prions in the placenta, whatever the genotype of their progeny. Multinucleated trophoblast cells appeared to play a key role in placental PrPSc accumulation. PrPSc accumulation began in syncytiotrophoblasts before disseminating to uninucleated trophoblasts. As these result from trophoblast/uterine epithelial cell fusion, syncytiotrophoblast cells expressed maternal and fetal PrPC, whilst uninucleated trophoblast cells only expressed fetal PrPC. In ARR/VRQ scrapie-infected ewes, expression of the ARR allele by syncytiotrophoblasts appeared to prevent initiation of PrPSc placental deposition. The absence of prions in affected ARR/VRQ sheep placentae reinforces strongly the interest in ARR selection for scrapie control.
authors.affiliation: UMR INRA ENVT 1225, Interactions Hôte Agent Pathogène, Ecole Nationale Vétérinaire de Toulouse, 23 Chemin des Capelles, 31076 Toulouse, France.
authors.forename: C
authors.initials: C
authors.lastname: Lacroux
authors.affiliation: UMR INRA ENVT 1225, Interactions Hôte Agent Pathogène, Ecole Nationale Vétérinaire de Toulouse, 23 Chemin des Capelles, 31076 Toulouse, France.
authors.forename: F
authors.initials: F
authors.lastname: Corbière
authors.affiliation: UMR INRA ENVT 1225, Interactions Hôte Agent Pathogène, Ecole Nationale Vétérinaire de Toulouse, 23 Chemin des Capelles, 31076 Toulouse, France.
authors.forename: G
authors.initials: G
authors.lastname: Tabouret
authors.affiliation: UMR INRA ENVT 1225, Interactions Hôte Agent Pathogène, Ecole Nationale Vétérinaire de Toulouse, 23 Chemin des Capelles, 31076 Toulouse, France.
authors.forename: S
authors.initials: S
authors.lastname: Lugan
authors.affiliation: UMR INRA ENVT 1225, Interactions Hôte Agent Pathogène, Ecole Nationale Vétérinaire de Toulouse, 23 Chemin des Capelles, 31076 Toulouse, France.
authors.forename: P
authors.initials: P
authors.lastname: Costes
authors.affiliation: UMR INRA ENVT 1225, Interactions Hôte Agent Pathogène, Ecole Nationale Vétérinaire de Toulouse, 23 Chemin des Capelles, 31076 Toulouse, France.
authors.forename: J
authors.initials: J
authors.lastname: Mathey
authors.affiliation: UMR INRA ENVT 1225, Interactions Hôte Agent Pathogène, Ecole Nationale Vétérinaire de Toulouse, 23 Chemin des Capelles, 31076 Toulouse, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Delmas
authors.affiliation: INRA Domaine de Langlade, 31450 Pompertuzat, France.
authors.forename: J L
authors.initials: JL
authors.lastname: Weisbecker
authors.affiliation: UMR INRA ENVT 1225, Interactions Hôte Agent Pathogène, Ecole Nationale Vétérinaire de Toulouse, 23 Chemin des Capelles, 31076 Toulouse, France.
authors.forename: G
authors.initials: G
authors.lastname: Foucras
authors.affiliation: UMR INRA ENVT 1225, Interactions Hôte Agent Pathogène, Ecole Nationale Vétérinaire de Toulouse, 23 Chemin des Capelles, 31076 Toulouse, France.
authors.forename: H
authors.initials: H
authors.lastname: Cassard
authors.affiliation: INRA Station d'Amélioration Génétique des Animaux, 31326 Castanet Tolosan, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Elsen
authors.affiliation: UMR INRA ENVT 1225, Interactions Hôte Agent Pathogène, Ecole Nationale Vétérinaire de Toulouse, 23 Chemin des Capelles, 31076 Toulouse, France.
authors.forename: F
authors.initials: F
authors.lastname: Schelcher
authors.affiliation: UMR INRA ENVT 1225, Interactions Hôte Agent Pathogène, Ecole Nationale Vétérinaire de Toulouse, 23 Chemin des Capelles, 31076 Toulouse, France.
authors.forename: O
authors.initials: O
authors.lastname: Andréoletti
mesh_terms: D000483:Alleles; D000818:Animals; D005260:Female; D005333:Fetus; D005838:Genotype; D007150:Immunohistochemistry; D010920:Placenta / Q000737:chemistry* / Q000473:pathology; D018620:PrPSc Proteins / Q000032:analysis*; D011247:Pregnancy; D011248:Pregnancy Complications / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D012608:Scrapie / Q000378:metabolism* / Q000473:pathology; D012756:Sheep; D014327:Trophoblasts / Q000737:chemistry
chemical_list: D018620:PrPSc Proteins