[Estimation of blood-group-dependent hyperphosphatasemia in healthy subjects by agarose gel electrophoresis].
Intestinal alkaline phosphatase (IAP) isozymes in the healthy human sera appears in two isoforms: normal-molecular-weight IAP (NIAP) and high-molecular-weight IAP (HIAP). We have demonstrated that the reference range for ALP activity is higher in blood group B and O secretors than in the other blood groups, for the appearance of these isoforms is depended on blood group B or O secretors. We currently reported that blood groups dependent hyperphosphatasemia in healthy subjects only appeared in blood group B or O secretors. In the present paper, we examined estimation of blood groups dependent hyperphosphatasemia in healthy subjects by use of agarose gel electrophoresis. We classified 104 healthy subjects with the B or O secretors into two groups of blood groups dependent hyperphosphatasemia (n = 5) and normal ALP activity (n = 99). The mean HIAP percentage in blood groups dependent hyperphosphatasemia group (18.5%) was higher than that in normal ALP activity group (9.9%). These results suggested that blood groups dependent hyperphosphatasemia in healthy subjects can be estimated from the HIAP levels by agarose gel electrophoresis.
Matsushita
Makoto
M
Department of Clinical Chemistry, Saitama Prefectural University, Koshigaya.
Irino
Tsutomu
T
Kase
Hitomi
H
Kamiyama
Kiyoshi
K
Komoda
Tsugikazu
T
D000017:ABO Blood-Group System; D000328:Adult; D000368:Aged; D000469:Alkaline Phosphatase / Q000097:blood* / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D006801:Humans; D007527:Isoenzymes / Q000097:blood / Q000302:isolation & purification; D008875:Middle Aged; D008970:Molecular Weight
D000017:ABO Blood-Group System; D007527:Isoenzymes; D000469:Alkaline Phosphatase