SELEX and dynamic combinatorial chemistry interplay for the selection of conjugated RNA aptamers.
SELEX (for Systematic Evolution of Ligands by Exponential enrichment) has proven to be extraordinarily powerful for the isolation of DNA or RNA aptamers that bind with high affinity and specificity to a wide range of molecular targets. However, the modest chemical functionality of nucleic acids poses some limits on the versatility of aptamers as binders and catalysts. To further improve the properties of aptamers, additional chemical diversity must be introduced. The design of chemical modifications is not a trivial task. Recently, dynamic combinatorial chemistry (DCC) has been introduced as an alternative to traditional combinatorial chemistry. DCC employs equilibrium shifting to effect molecular evolution of a dynamic combinatorial library of molecules. Herein, we describe an original process that combines DCC and SELEX for the in vitro selection of modified aptamers which are conjugated to chemically diverse small-molecules. Its successful application for the selection of small-molecule conjugated RNA aptamers that bind tightly to the transactivation-response (TAR) element of HIV-1 is presented.
Bugaut
Anthony
A
INSERM U386, 146 rue Leo Saignat, 33076 BORDEAUX Cedex, France. ab605@cam.ac.uk
Toulmé
Jean-Jacques
JJ
Rayner
Bernard
B
D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D001483:Base Sequence; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D019020:Directed Molecular Evolution; D006678:HIV / Q000737:chemistry; D016325:HIV Long Terminal Repeat / Q000187:drug effects; D008969:Molecular Sequence Data; D015394:Molecular Structure; D012367:RNA, Viral / Q000737:chemistry*; D052156:SELEX Aptamer Technique / Q000379:methods*; D017423:Sequence Analysis, RNA / Q000379:methods
D052157:Aptamers, Nucleotide; D012367:RNA, Viral