Overexpression of Arabidopsis AGD7 causes relocation of Golgi-localized proteins to the endoplasmic reticulum and inhibits protein trafficking in plant cells.
ADP ribosylation factor (Arf) GTPase-activating proteins (GAPs) promote the hydrolysis of GTP bound to Arfs to GDP, which plays a pivotal role in regulating Arfs by converting the active GTP-bound forms of these proteins into their inactive GDP-bound forms. Here, we investigated the biological role of AGD7, an Arf GAP homolog, in Arabidopsis (Arabidopsis thaliana). We show that AGD7 bears a highly conserved N-terminal region and a unique C-terminal region, interacts with Arf1 both in vitro and in vivo, and stimulates Arf1 GTPase activity in a phosphatidic acid-dependent manner in vitro. In plant cells, AGD7 localized to the Golgi complex, where its overexpression was found to inhibit the Golgi localization of gamma-subunit of coat proteins and promote the relocation of Golgi proteins into the endoplasmic reticulum in both protoplasts and transgenic plants. Furthermore, overexpression of AGD7 inhibited anterograde trafficking of proteins from the endoplasmic reticulum. We propose that AGD7 functions as a GAP for Arf1 in the Golgi complex and plays a critical role in protein trafficking by controlling Arf1 activity.
Min
Myung Ki
MK
Center for Plant Intracellular Trafficking, Division of Molecular and Life Sciences, and Department of Biology, Pohang University of Science and Technology, Pohang, 790-784, Korea.
Kim
Soo Jin
SJ
Miao
Yansong
Y
Shin
Juyoun
J
Jiang
Liwen
L
Hwang
Inhwan
I
D017360:Arabidopsis / Q000166:cytology / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D001483:Base Sequence; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D020558:GTP Phosphohydrolases / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism*; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D021381:Protein Transport; D014157:Transcription Factors / Q000378:metabolism
C503841:ARF1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D017931:DNA Primers; D004268:DNA-Binding Proteins; D014157:Transcription Factors; D020558:GTP Phosphohydrolases