Synthesis of two carboxylic haptens for raising antibodies to 25-hydroxyvitamin D3 and 1alpha,25-dihydroxyvitamin D3.
Hapten derivatives of 25-hydroxyvitamin D(3) and 1alpha,25-dihydroxyvitamin D(3) were synthesized using the Wittig-Horner approach. Both haptens bearing a carboxylic group at the side chain that can be linked to a protein for raising antibodies of potential utility for the determination of 25-hydroxyvitamin D(3), 1alpha,25-dihydroxyvitamin D(3) and 1alpha-hydroxylated vitamin D(3) analogues.
Gregorio
Carlos
C
Departamento de Química Orgánica y Unidad Asociada al C.S.I.C., Universidad de Santiago de Compostela, E-15706 Santiago de Compostela, Spain.
Eduardo
Silvina
S
Rodrigues
Luis C
LC
Regueira
María A
MA
Fraga
Ramón
R
Riveiros
Ricardo
R
Maestro
Miguel
M
Mouriño
Antonio
A
D002112:Calcifediol / Q000138:chemical synthesis* / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D006241:Haptens / Q000737:chemistry* / Q000276:immunology; D015394:Molecular Structure; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology / Q000378:metabolism
D006241:Haptens; C118756:dihydroxy-vitamin D3; D014807:Vitamin D; D002112:Calcifediol