title: The role of myelin in Theiler's virus persistence in the central nervous system.
abstract: Theiler's virus, a picornavirus, persists for life in the central nervous system of mouse and causes a demyelinating disease that is a model for multiple sclerosis. The virus infects neurons first but persists in white matter glial cells, mainly oligodendrocytes and macrophages. The mechanism, by which the virus traffics from neurons to glial cells, and the respective roles of oligodendrocytes and macrophages in persistence are poorly understood. We took advantage of our previous finding that the shiverer mouse, a mutant with a deletion in the myelin basic protein gene (Mbp), is resistant to persistent infection to examine the role of myelin in persistence. Using immune chimeras, we show that resistance is not mediated by immune responses or by an efficient recruitment of inflammatory cells into the central nervous system. With both in vivo and in vitro experiments, we show that the mutation does not impair the permissiveness of neurons, oligodendrocytes, and macrophages to the virus. We demonstrate that viral antigens are present in cytoplasmic channels of myelin during persistent infection of wild-type mice. Using the optic nerve as a model, we show that the virus traffics from the axons of retinal ganglion cells to the cytoplasmic channels of myelin, and that this traffic is impaired by the shiverer mutation. These results uncover an unsuspected axon to myelin traffic of Theiler's virus and the essential role played by the infection of myelin/oligodendrocyte in persistence.
authors.affiliation: Unité des Virus Lents, Département de Virologie, Institut Pasteur and Centre National de la Recherche Scientifique, Paris, France.
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Roussarie
authors.forename: Claude
authors.initials: C
authors.lastname: Ruffié
authors.forename: Michel
authors.initials: M
authors.lastname: Brahic
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001854:Bone Marrow Cells / Q000502:physiology; D001921:Brain / Q000821:virology*; D018188:Cardiovirus Infections / Q000276:immunology; D002478:Cells, Cultured; D051379:Mice; D008809:Mice, Inbred C3H; D009154:Mutation; D009186:Myelin Sheath / Q000235:genetics / Q000502:physiology*; D009836:Oligodendroglia / Q000821:virology; D017945:Theilovirus / Q000254:growth & development* / Q000472:pathogenicity
chemical_list: D000956:Antigens, Viral