title: Nitric oxide and oxygen radical attack on GDP-dissociation inhibitor 2 (GDI-2) in spinal cord injury of the rat.
abstract: Protein oxidation and nitration have been described during spinal cord injury (SCI) in animal models. Herein, mass spectrometry unambiguously identified GDP-dissociation inhibitor-2 (GDI-2) in SCI with post-translational modifications of 3-aminotyrosine (8 h post-injury) and an acrolein adduct of GDI-2 (72 h post-injury). On the basis of mass spectrometry evidence, we conclude that lipid-peroxidation and protein nitration do take place on an important signalling protein that may be prevented by specific experimental therapeutic interventions.
authors.affiliation: Department of Pediatrics, Medical University of Vienna, Vienna, Austria.
authors.forename: Julius Paul Pradeep
authors.initials: JP
authors.lastname: John
authors.forename: Oliver
authors.initials: O
authors.lastname: Pintsov
authors.forename: Alexander
authors.initials: A
authors.lastname: Petter-Puchner
authors.forename: Heinz
authors.initials: H
authors.lastname: Redl
authors.forename: Arnold
authors.initials: A
authors.lastname: Pollak
authors.forename: Wei-Qiang
authors.initials: WQ
authors.lastname: Chen
authors.forename: Gert
authors.initials: G
authors.lastname: Lubec
mesh_terms: D000171:Acrolein / Q000032:analysis; D000595:Amino Acid Sequence; D000818:Animals; D020730:Guanine Nucleotide Dissociation Inhibitors / Q000737:chemistry / Q000378:metabolism*; D015227:Lipid Peroxidation; D008297:Male; D008969:Molecular Sequence Data; D009569:Nitric Oxide / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011499:Protein Processing, Post-Translational; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013119:Spinal Cord Injuries / Q000378:metabolism*; D014443:Tyrosine / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: C521203:Gdi2 protein, rat; D020730:Guanine Nucleotide Dissociation Inhibitors; D017382:Reactive Oxygen Species; C019253:3-aminotyrosine; D009569:Nitric Oxide; D014443:Tyrosine; D000171:Acrolein