Microbiological outcomes of quadrant versus full-mouth root planing as monitored by real-time PCR.
OBJECTIVE
To study the short-term microbiological changes following full-mouth compared with quadrant wise scaling and root planing (FMRP and QRP) as well as long-term effects.


METHODS
Twenty patients with chronic periodontitis were randomized into a test group treated in two sessions with subgingival scaling and root planing within 24 h (FMRP) and a control group treated quadrant by quadrant in four sessions at intervals of one week (QRP). Microbiological samples were taken in the two deepest pockets of the maxillary right quadrant immediately before treatment and after 1 day, 1, 2, 4, 8, 12, and 24 weeks. The samples were evaluated by real-time PCR for quantification of Actinobacillus actinomycetemcomitans, Fusobacterium nucleatum ssp., Porphyromonas gingivalis, Prevotella intermedia, Treponema denticola, and Tannerella forsythia as well as for total bacterial counts (TBC).


RESULTS
Treatment resulted in a TBC median log reduction of 0.75 (FMRP) and 0.72 (QRP). There were no differences between groups either for the short term (1 day-4 weeks) (analysis of variance: p=0.3150) or for long term (4-24 weeks) (analysis of variance: p=0.9671). Likewise, no differences were detected for selected target bacteria.


CONCLUSIONS
The results of the present study showed similar microbiological outcomes following both treatment modalities.
Jervøe-Storm
P-M
PM
Department of Periodontology, Operative and Preventive Dentistry, University of Bonn, Bonn, Germany. storm@uni-bonn.de
AlAhdab
H
H
Semaan
E
E
Fimmers
R
R
Jepsen
S
S
D000328:Adult; D016976:Aggregatibacter actinomycetemcomitans / Q000235:genetics / Q000302:isolation & purification; D000704:Analysis of Variance; D015373:Bacterial Typing Techniques; D002908:Chronic Disease; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000032:analysis; D003773:Dental Plaque / Q000382:microbiology / Q000517:prevention & control; D012534:Dental Scaling / Q000379:methods*; D005260:Female; D005500:Follow-Up Studies; D016967:Fusobacterium nucleatum / Q000235:genetics / Q000302:isolation & purification; D006090:Gram-Negative Bacteria / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010514:Periodontal Pocket / Q000382:microbiology / Q000628:therapy; D010518:Periodontitis / Q000382:microbiology / Q000628:therapy*; D016133:Polymerase Chain Reaction; D016966:Porphyromonas gingivalis / Q000235:genetics / Q000302:isolation & purification; D018713:Prevotella intermedia / Q000235:genetics / Q000302:isolation & purification; D016745:Root Planing / Q000379:methods*; D013357:Subgingival Curettage / Q000379:methods; D016896:Treatment Outcome; D048373:Treponema denticola / Q000235:genetics / Q000302:isolation & purification
D004269:DNA, Bacterial