Clone-specific anti-CD45 blocking factor in patient plasma.
We observed inhibition of anti-CD45, but not anti-CD3, -CD4, or -CD8 staining in the whole blood of an individual clinical specimen. Subsequent experiments demonstrated the presence of a plasma factor, which likewise blocked CD45 staining of third party lymphocytes. The blocking activity was anti-CD45 clone (2D1) specific. Experiments utilizing immunoabsorption, immunofluorescence, and commercial blocking reagents failed to provide evidence for identifying blocking factor as heterophilic antibody. Biochemical identification of blocking factor was not accomplished.
Wellhausen
Samuel R
SR
Flow Cytometry Laboratory, James Graham Brown Cancer Center, University of Louisville, School of Medicine, Louisville, Kentucky 40202, USA. samuel.wellhausen@louisville.edu
Slone
Stephen P
SP
Miller
James J
JJ
D000328:Adult; D000910:Antibodies, Heterophile / Q000097:blood / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000097:blood / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D015658:HIV Infections / Q000097:blood / Q000276:immunology*; D006801:Humans; D007163:Immunosorbent Techniques; D017493:Leukocyte Common Antigens / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology / Q000821:virology
D000910:Antibodies, Heterophile; D000911:Antibodies, Monoclonal; D017493:Leukocyte Common Antigens; C516769:PTPRC protein, human