Liquid chromatography tandem mass spectrometry assay to determine the pharmacokinetics of aildenafil in human plasma.
A simple, sensitive and specific liquid chromatography/tandem mass spectrometry method for the quantitation of aildenafil, a new phosphodiesterase V inhibitor, in human plasma is presented. The analyte and internal standard, sildenafil, were extracted by a one-step liquid-liquid extraction in alkaline conditions and separated on a C(18) column using ammonia:10mM ammonium acetate buffer:methanol (0.1:15:85, v/v/v) as the mobile phase. The detection by an API 4000 triple quadrupole mass spectrometer in multiple-reaction monitoring mode was completed within 2.5 min. The calibration curve exhibited a linear dynamic range of 0.05-100 ng/ml with a 10 pg/ml limit of detection. The intra- and inter-day precisions measured as relative standard deviation were within 8.04% and 5.72%, respectively. This method has been used in a pharmacokinetic study of aildenafil in healthy male volunteers each given an oral administration of one of the three dosages.
Wang
Jiang
J
Research Center for Drug Metabolism, College of Life Science, Jilin University, Changchun 130023, China.
Jiang
Yao
Y
Wang
Yingwu
Y
Zhao
Xia
X
Cui
Yimin
Y
Gu
Jingkai
J
D000085:Acetates / Q000737:chemistry; D000284:Administration, Oral; D000641:Ammonia / Q000737:chemistry; D019540:Area Under Curve; D002021:Buffers; D002138:Calibration; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D005215:Fasting; D006207:Half-Life; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008657:Metabolic Clearance Rate; D000432:Methanol / Q000737:chemistry; D015394:Molecular Structure; D010726:Phosphodiesterase Inhibitors / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification / Q000493:pharmacokinetics*; D010879:Piperazines / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D011687:Purines; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D000068677:Sildenafil Citrate; D013450:Sulfones / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D013607:Tablets; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors
D000085:Acetates; D002021:Buffers; D010726:Phosphodiesterase Inhibitors; D010879:Piperazines; D011687:Purines; D013450:Sulfones; D013607:Tablets; D000641:Ammonia; C520604:aildenafil; D000068677:Sildenafil Citrate; C018824:ammonium acetate; D000432:Methanol