The same dorsal root ganglion neurons innervate uterus and colon in the rat.
The purpose of this study was to determine whether primary sensory neurons that innervate the uterus receive convergent input from the colon. To test this, in the rat, cell bodies of colonic and uterine dorsal root ganglia were retrogradely labeled with fluorescent tracer dyes microinjected into the colon/rectum and bilaterally into the uterine horns. Ganglia were harvested, cryoprotected and cut into 20 microm slices to identify positively stained cells for fluorescent microscopy. Up to 5% of neurons were colon-specific or uterus-specific, and 10-15% of labeled ganglion neurons innervate both viscera in the L1, L2, L6 and S1-S3 levels. These results suggest a novel form of visceral sensory integration in the dorsal root ganglion that may underlie comorbidity of many functional pain syndromes.
Chaban
Victor
V
Department of Neurobiology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA. chaban@ucla.edu
Christensen
Amy
A
Wakamatsu
Micah
M
McDonald
Michelle
M
Rapkin
Andrea
A
McDonald
John
J
Micevych
Paul
P
D000344:Afferent Pathways / Q000166:cytology / Q000502:physiology; D000818:Animals; D003106:Colon / Q000294:innervation* / Q000502:physiology; D005260:Female; D005456:Fluorescent Dyes; D005727:Ganglia, Spinal / Q000166:cytology* / Q000502:physiology; D007001:Hypogastric Plexus / Q000166:cytology / Q000502:physiology; D018836:Inflammation Mediators / Q000378:metabolism; D009475:Neurons, Afferent / Q000166:cytology* / Q000502:physiology; D009619:Nociceptors / Q000166:cytology / Q000502:physiology; D017699:Pelvic Pain / Q000503:physiopathology; D051381:Rats; D020318:Rats, Long-Evans; D014599:Uterus / Q000294:innervation* / Q000502:physiology; D017833:Visceral Afferents / Q000033:anatomy & histology* / Q000502:physiology
D005456:Fluorescent Dyes; D018836:Inflammation Mediators