title: A new cell line from larval fat bodies of the bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae).
abstract: A new cell line, designated IOZCAS-Ha-I, was initiated from the fat body of larvae of Helicoverpa armigera (Lepidoptera: Noctuidae) in TNM-FH medium containing 10% fetal bovine serum. Spherical cells were predominant among the various cell types. The cell line showed a typical lepidopteran chromosome pattern ranging from 58 to 239 chromosomes in the majority of the cells. It was confirmed to have originated from the H. armigera by the DNA amplification- fingerprinting polymerase chain reaction (DAF-PCR) technique. The new cell line was only slightly susceptible to the multiple nucleocapsid nuclear polyhedrosis viruses (NPV) from H. armigera.
authors.affiliation: State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Zhang
authors.forename: Yong-An
authors.initials: YA
authors.lastname: Zhang
authors.forename: Qilian
authors.initials: Q
authors.lastname: Qin
authors.forename: Yuzhu
authors.initials: Y
authors.lastname: Wang
authors.forename: Xuan
authors.initials: X
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Miao
authors.forename: Zhenxian
authors.initials: Z
authors.lastname: Yin
authors.forename: Aijun
authors.initials: A
authors.lastname: Zhang
authors.forename: Liangjian
authors.initials: L
authors.lastname: Qu
authors.forename: Cui
authors.initials: C
authors.lastname: Ding
mesh_terms: D000818:Animals; D002460:Cell Line; D002875:Chromosomes / Q000235:genetics; D004247:DNA / Q000032:analysis; D005216:Fat Body / Q000166:cytology*; D002479:Inclusion Bodies; D007814:Larva / Q000166:cytology; D007915:Lepidoptera / Q000166:cytology*
chemical_list: D004247:DNA