Structural analysis of an acidic, fatty acid ester-bonded extracellular polysaccharide produced by a pristane-assimilating marine bacterium, Rhodococcus erythropolis PR4.
Rhodococcus erythropolis PR4 is a marine bacterium that can degrade various alkanes including pristane, a C(19) branched alkane. This strain produces a large quantity of extracellular polysaccharides, which are assumed to play an important role in the hydrocarbon tolerance of this bacterium. The strain produced two acidic extracellular polysaccharides, FR1 and FR2, and the latter showed emulsifying activity toward clove oil, whereas the former did not. FR2 was composed of D-galactose, D-glucose, D-mannose, D-glucuronic acid, and pyruvic acid at a molar ratio of 1:1:1:1:1, and contained 2.9% (w/w) stearic acid and 4.3% (w/w) palmitic acid attached via ester bonds. Therefore, we designated FR2 as a PR4 fatty acid-containing extracellular polysaccharide or FACEPS. The chemical structure of the PR4 FACEPS polysaccharide chain was determined by 1D (1)H and (13)C NMR spectroscopies as well as by 2D DQF-COSY, TOCSY, HMQC, HMBC, and NOESY experiments. The sugar chain of PR4 FACEPS was shown to consist of tetrasaccharide repeating units having the following structure: [structure: see text].
Urai
Makoto
M
Laboratory of Molecular Microbiology, Department of Applied Biological Science, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa 252-8510, Japan.
Yoshizaki
Hiroshi
H
Anzai
Hirosi
H
Ogihara
Jun
J
Iwabuchi
Noriyuki
N
Harayama
Shigeaki
S
Sunairi
Michio
M
Nakajima
Mutsuyasu
M
D002240:Carbohydrate Sequence; D005110:Extracellular Space / Q000737:chemistry* / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008745:Methylation; D009005:Monosaccharides / Q000032:analysis / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000378:metabolism*; D012240:Rhodococcus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012623:Seawater / Q000382:microbiology*; D013729:Terpenes / Q000378:metabolism*
D005227:Fatty Acids; D009005:Monosaccharides; D011135:Polysaccharides, Bacterial; D013729:Terpenes; C009042:pristane