A water-soluble, octacationic zinc phthalocyanine as molecular probe for nucleic acid secondary structure.
The interaction between CT-DNA and the zinc phthalocyanine ZnPc (1) was studied by UV/VIS and fluorescence titration, as well as by thermal denaturation. ZnPc was found to strongly bind to CT-DNA (K(app)=7.35 x 10(5) M(-1)) in a non-intercalative mode. The photosensitized cleavage of pBR322 DNA was found to efficiently proceed via singlet-oxygen ((1)O(2)) production. Further, ZnPc (1) caused site-specific scission of guanine (G) bases around the bulge of the hairpin oligonucleotides OD1-OD3, as clearly shown by gel-electrophoresis experiments.
Zhang
An-Ming
AM
College of Chemistry and Molecular Sciences, Wuhan University, Hubei, Wuhan, PR China.
Huang
Jing
J
Weng
Xiao-Cheng
XC
Li
Jin-Xi
JX
Ren
Li-Ge
LG
Song
Zhi-Bin
ZB
Xiong
Xiao-Qin
XQ
Zhou
Xiang
X
Cao
Xiao-Ping
XP
Zhou
Yan
Y
D004247:DNA / Q000378:metabolism*; D007211:Indoles / Q000378:metabolism*; D054833:Isoindoles; D015335:Molecular Probes; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009942:Organometallic Compounds / Q000378:metabolism*; D010782:Photolysis; D013056:Spectrophotometry, Ultraviolet; D017967:Zinc Compounds
D007211:Indoles; D054833:Isoindoles; D015335:Molecular Probes; D009942:Organometallic Compounds; D017967:Zinc Compounds; C052159:Zn(II)-phthalocyanine; D004247:DNA