title: Genomic DNA isolation from different biological materials.
abstract: A comprehensive collection of different methods for extracting high-quality genomic DNA from gram-positive and -negative bacteria and fungal mycelium and spores is described in this chapter. Special care has been taken in describing the ideal ratio of biological material to chemical reagents for an efficient extraction of genomic DNA, and in stating the appropriate application in molecular biology protocols (e.g., PCR or genomic DNA library-cloning quality).
authors.affiliation: Ecological Genetics Laboratory, Landcare Research Ltd., Auckland, New Zealand.
authors.forename: Duckchul
authors.initials: D
authors.lastname: Park
mesh_terms: D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000235:genetics* / Q000302:isolation & purification*; D005658:Fungi / Q000235:genetics / Q000254:growth & development; D006090:Gram-Negative Bacteria / Q000235:genetics; D006094:Gram-Positive Bacteria / Q000235:genetics; D008828:Microbiological Techniques / Q000379:methods; D008967:Molecular Biology / Q000379:methods; D019800:Phenol; D013172:Spores, Fungal / Q000235:genetics
chemical_list: D002593:Cetrimonium Compounds; D003470:Culture Media; D004269:DNA, Bacterial; D004271:DNA, Fungal; D019800:Phenol; D000077286:Cetrimonium