title: Polyphasic approach to the taxonomy of the Rhizopus stolonifer group.
abstract: Phylogenetic analysis of Rhizopus strains based on the D1/D2 region of LSU rDNA sequences yielded a phylogram with four well-supported clades. The R. microsporus clade concurs with classification obtained by traditional methods. The R. oryzae group was found to include species of the genus Amylomyces. The traditional R. stolonifer group was divided into two well-supported clades in the phylogram, with one clade comprising R. stolonifer var. stolonifer, R. sexualis var. sexualis, and R. sexualis var. americanus and the other clade comprising taxa with recurved sporangiophores; R. reflexus, R. stolonifer var. lyococcus, and R. circinans, identifying recurved sporangiophores as an important taxonomic character. The molecular data supported the recognition of this clade at the species level: R. lyococcus (basionym: Sporotrichum lyococcum).
authors.affiliation: Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, Taiwan. gyl@firdi.org.tw
authors.forename: Guey-Yuh
authors.initials: GY
authors.lastname: Liou
authors.forename: Shyue-Ru
authors.initials: SR
authors.lastname: Chen
authors.forename: Yu-Hui
authors.initials: YH
authors.lastname: Wei
authors.forename: Fwu-Ling
authors.initials: FL
authors.lastname: Lee
authors.forename: Hui-Mei
authors.initials: HM
authors.lastname: Fu
authors.forename: Gwo-Fang
authors.initials: GF
authors.lastname: Yuan
authors.forename: Joost A
authors.initials: JA
authors.lastname: Stalpers
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D002965:Classification; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D005516:Food Microbiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012339:RNA, Ribosomal, 28S / Q000737:chemistry / Q000235:genetics; D012233:Rhizopus / Q000145:classification* / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D004271:DNA, Fungal; D012339:RNA, Ribosomal, 28S