Almost forgotten or latest practice? AFLP applications, analyses and advances.
Amplified fragment length polymorphism (AFLP) DNA fingerprinting is a firmly established molecular marker technique, with broad applications in population genetics, shallow phylogenetics, linkage mapping, parentage analyses, and single-locus PCR marker development. Technical advances have presented new opportunities for data analysis, and recent studies have addressed specific areas of the AFLP technique, including comparison to other genotyping methods, assessment of errors, homoplasy, phylogenetic signal and appropriate analysis techniques. Here we provide a synthesis of these areas and explore new directions for the AFLP technique in the genomic era, with the aim of providing a review that will be applicable to all AFLP-based studies.
Meudt
Heidi M
HM
Allan Wilson Centre for Molecular Ecology and Evolution, Massey University, Private Bag 11222, Palmerston North 4442, New Zealand. heidim@tepapa.govt.nz
Clarke
Andrew C
AC
D000818:Animals; D002874:Chromosome Mapping; D016172:DNA Fingerprinting; D005838:Genotype; D010802:Phylogeny; D010944:Plants / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length