Protein carbonyls as a biomarker of foetal-neonatal hypoxic stress.
OBJECTIVE
Investigation of the effect of hypoxic conditions during labour on the protein oxidative modifications and changes in plasma antioxidative capacity of newborns.


METHODS
Oxidative damage to proteins was determined by high-performance liquid chromatography. Antioxidative status was monitored by Trolox equivalent antioxidant capacity method. In our study, 11 hypoxic and 19 normoxic newborns were involved.


RESULTS
In hypoxic newborns, we have found a significant increase in protein carbonyl levels (3.55+/-0.86 versus 3.24+/-0.69 mol carbonyls/mol proteins, p=0.045) and plasma antioxidant capacity (1.76+/-0.056 versus 1.68+/-0.097 mmol Trolox/L, p=0.004) when compared to normoxic children. Bilirubin levels were unchanged (p=0.87).


CONCLUSIONS
Our results show elevated levels of carbonyls in hypoxic neonates compared to normoxic children. The oxidative damage to proteins is not sufficiently prevented by increased antioxidant capacity detected in plasma of hypoxic newborns.
Zitnanová
Ingrid
I
Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Sasinkova 2, Bratislava, Slovak Republic.
Sumegová
Katarína
K
Simko
Martin
M
Maruniaková
Alexandra
A
Chovanová
Zuzana
Z
Chavko
Mikulás
M
Duracková
Zdenka
Z
D000975:Antioxidants / Q000378:metabolism; D001663:Bilirubin / Q000378:metabolism; D015415:Biomarkers / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D005312:Fetal Blood / Q000378:metabolism*; D006801:Humans; D000860:Hypoxia / Q000503:physiopathology; D007231:Infant, Newborn; D010084:Oxidation-Reduction; D050050:Protein Carbonylation; D011506:Proteins / Q000032:analysis* / Q000378:metabolism
D000975:Antioxidants; D015415:Biomarkers; D011506:Proteins; D001663:Bilirubin