Leishmania amazonensis: Anionic currents expressed in oocytes upon microinjection of mRNA from the parasite.
Transport mechanisms involved in pH homeostasis are relevant for the survival of Leishmania parasites. The presence of chloride conductive pathways in Leishmania has been anticipated since anion channel inhibitors limit the proton extrusion mediated by the H+ATPase, which is the major regulator of intracellular pH in amastigotes. In this study, we used Xenopus laevis oocytes as a heterologous expression system in which to study the expression of ion channels upon microinjection of polyA mRNA from Leishmania amazonensis. After injection of polyA mRNA into the oocytes, we measured three different types of currents. We discuss the possible origin of each, and propose that Type 3 currents could be the result of the heterologous expression of proteins from Leishmania since they show different pharmacological and biophysical properties as compared to endogenous oocyte currents.
Lagos M
Luisa F
LF
Facultad de Salud, Programa de Doctorado en Ciencias Biomédicas, Universidad del Valle, Cali, Colombia.
Moran
Oscar
O
Camacho
Marcela
M
D000818:Animals; D006863:Hydrogen-Ion Concentration; D007894:Leishmania mexicana / Q000737:chemistry / Q000235:genetics*; D008264:Macrophages / Q000737:chemistry / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects; D008845:Microinjections; D009865:Oocytes / Q000502:physiology*; D018408:Patch-Clamp Techniques; D011061:Poly A / Q000235:genetics*; D012333:RNA, Messenger / Q000008:administration & dosage* / Q000494:pharmacology / Q000502:physiology; D016053:RNA, Protozoan / Q000008:administration & dosage / Q000494:pharmacology / Q000502:physiology; D050994:Voltage-Dependent Anion Channels / Q000187:drug effects / Q000502:physiology*; D014982:Xenopus laevis
D012333:RNA, Messenger; D016053:RNA, Protozoan; D050994:Voltage-Dependent Anion Channels; D011061:Poly A