title: Reference strand-mediated conformation analysis-based typing of multiple alleles in the rhesus macaque MHC class I Mamu-A and Mamu-B loci.
abstract: The rhesus macaque exhibits individual differences in susceptibility and resistance to infectious agents such as simian immunodeficiency virus (SIV) under experimental conditions, and these may be genetically determined at least in part by major histocompatibility complex (MHC) class I polymorphism. Although the importance of defining MHC class I polymorphism is well recognized, development of a generic and comprehensive molecular typing method of MHC class I alleles of the rhesus macaque has been hampered because, during the evolution of this species, multiple copies of similar DNA sequences have been generated by duplication events including the coding sequences of Mamu-A and Mamu-B loci. We report here a newly developed reference strand-mediated conformation analysis (RSCA)-based typing method of multiple Mamu-A and Mamu-B cDNAs that allowed us to estimate the number of expressed alleles. This technique detected 1-7 Mamu-A signals and 2-12 Mamu-B signals in a single sample, indicating that the number of functional alleles may vary. By comparing the data from the parents with those from the descendants in the breeding colony, several MHC class I haplotypes consisting of variable numbers of functional Mamu-A and Mamu-B alleles could be assigned.
authors.affiliation: Department of Molecular Pathogenesis, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Tanaka-Takahashi
authors.forename: Michio
authors.initials: M
authors.lastname: Yasunami
authors.forename: Taeko
authors.initials: T
authors.lastname: Naruse
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Hinohara
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Matano
authors.forename: Kazuyasu
authors.initials: K
authors.lastname: Mori
authors.forename: Masaaki
authors.initials: M
authors.lastname: Miyazawa
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Honda
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yasutomi
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Nagai
authors.forename: Akinori
authors.initials: A
authors.lastname: Kimura
mesh_terms: D000483:Alleles; D000818:Animals; D018984:Epitopes, T-Lymphocyte / Q000235:genetics*; D005805:Genes, MHC Class I / Q000235:genetics*; D006239:Haplotypes; D008253:Macaca mulatta / Q000235:genetics / Q000276:immunology*; D018807:Polymorphism, Single-Stranded Conformational; D016097:Simian Acquired Immunodeficiency Syndrome / Q000235:genetics*; D015302:Simian Immunodeficiency Virus / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D018984:Epitopes, T-Lymphocyte