Effect of both ultraviolet B irradiation and histamine receptor function on allergic responses to an inhaled antigen.
Exposure of skin to UVB radiation (290-320 nm) modulates the immune system, with most studies showing a suppression of Th1-driven immune responses. This study investigated the effects of UVB on Th2-associated immune responses using a murine model of allergic respiratory inflammation. C57BL/6, histamine receptor-1 knockout (H1RKO), and histamine receptor-2 knockout (H2RKO) mice were exposed to a single 4 kJ/m(2) dose of UVB (twice a minimal edemal dose) on shaved dorsal skin 3 days before intranasal sensitization with papain, a cysteine protease homologue of the dust mite allergen Der p 1. H1RKO mice demonstrated enhanced papain-specific inflammatory responses in the lung-draining lymph nodes (LDLNs), whereas the responses of H2RKO mice closely mimicked those of C57BL/6 mice. UVB irradiation 3 days before sensitization reduced in vitro papain-specific proliferation of LDLN cells of C57BL/6 and H1RKO mice but not H2RKO mice 24 h after challenge. The regulatory effect of UVB was transferred by adoptive transfer of unfractionated LDLN cells from UVB-irradiated, papain-sensitized C57BL/6 and H1RKO donor mice in naive recipients of the corresponding strain that were subsequently sensitized and challenged with papain. Additionally, UVB exposure suppressed papain-induced IL-5 and IL-10 production in vitro by LDLN cells from H1RKO mice but not from C57BL/6 mice or H2RKO mice. The results of this study demonstrate systemic immunomodulation of responses to intranasally delivered Ag by UVB irradiation and implicate a role for the H2 receptor in UVB-induced suppression of Ag-specific responses in the draining lymph nodes.
McGlade
Jacqueline P
JP
Telethon Institute for Child Health Research and Centre for Child Health Research, University of Western Australia, West Perth, WA, Australia.
Gorman
Shelley
S
Lenzo
Jason C
JC
Tan
Jamie W
JW
Watanabe
Takeshi
T
Finlay-Jones
John J
JJ
Thomas
Wayne R
WR
Hart
Prue H
PH
D000281:Administration, Intranasal; D019264:Adoptive Transfer; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000276:immunology*; D060829:Arthropod Proteins; D003546:Cysteine Endopeptidases; D005260:Female; D006967:Hypersensitivity / Q000276:immunology* / Q000517:prevention & control; D007108:Immune Tolerance / Q000528:radiation effects*; D007249:Inflammation / Q000276:immunology / Q000517:prevention & control; D008198:Lymph Nodes / Q000276:immunology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D010206:Papain / Q000276:immunology / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011968:Receptors, Histamine / Q000172:deficiency / Q000276:immunology*; D014466:Ultraviolet Rays
D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; D011968:Receptors, Histamine; D003546:Cysteine Endopeptidases; C451162:Dermatophagoides pteronyssinus antigen p 1; D010206:Papain