The transcriptional factor Osterix directly interacts with RNA helicase A.
Osterix is an osteoblast-specific transcriptional factor, required for bone formation and osteoblast differentiation. Here, we identified new Osterix interacting factors by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Among the candidates, RNA helicase A was identified to interact with Osterix. To determine the interaction of Osterix with RNA helicase A, immunoprecipitation assay was performed. Western analysis confirmed the association between Osterix and RNA helicase A. Immunocytochemical analysis also showed that Osterix and RNA helicase A were co-localized in HEK 293 cells. Our data suggest that RNA helicase A might be a component of Osterix regulation.
Amorim
Bruna Rabelo
BR
Department of Histology and Oral Histology, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima 770-8504, Japan.
Okamura
Hirohiko
H
Yoshida
Kaya
K
Qiu
Lihong
L
Morimoto
Hiroyuki
H
Haneji
Tatsuji
T
D000595:Amino Acid Sequence; D002460:Cell Line; D006801:Humans; D047468:Immunoprecipitation; D008969:Molecular Sequence Data; D011485:Protein Binding; D020365:RNA Helicases / Q000737:chemistry / Q000378:metabolism*; D000076182:Sp7 Transcription Factor; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014157:Transcription Factors / Q000378:metabolism*
D000076182:Sp7 Transcription Factor; C479466:Sp7 protein, mouse; D014157:Transcription Factors; D020365:RNA Helicases