Upregulation of Mark3 and Rpgrip1 mRNA expression by jujuboside A in mouse hippocampus.
OBJECTIVE
To investigate the effect of jujuboside A (JuA) on modulating gene expression in the hippocampus.


METHODS
The spontaneous activity of mice was monitored, and the differential display polymerase chain reaction was adapted to screen differentially-expressed genes modulated by JuA in the mouse hippocampus.


RESULTS
JuA significantly decreased the total activity intensity (P<0.01 vs control) at a dosage of 80 mg/kg, and the genes MAP/microtubule affinity-regulating kinase3 (Mark3) and retinitis pigmentosa GTPase regulator interacting protein1 (Rpgrip1) were upregulated by JuA in the mouse hippocampus.


CONCLUSIONS
JuA had an inhibitory effect on the spontaneous activity of the mice, and JuA regulated the transcription of Mark3 and Rpgrip1 in the mouse hippocampus.
Wang
Cheng
C
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China.
You
Zi-Li
ZL
Xia
Qing
Q
Xiong
Tao
T
Xia
Yang
Y
Yao
De-Zhong
DZ
D000818:Animals; D003598:Cytoskeletal Proteins; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D006993:Hypnotics and Sedatives / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009043:Motor Activity / Q000187:drug effects; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis*; D011506:Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012503:Saponins / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects
D003598:Cytoskeletal Proteins; D006993:Hypnotics and Sedatives; D011506:Proteins; D012333:RNA, Messenger; C540216:Rpgrip1 protein, mouse; D012503:Saponins; C087808:jujuboside A; C540215:Mark3 protein, mouse; D017346:Protein Serine-Threonine Kinases