Synchronization modulation of Na/K pump molecules can hyperpolarize the membrane resting potential in intact fibers.
Previously, we have theoretically studied the possibility of electrical rhythmic entrainment of carrier-mediated ion transporters, and experimentally realized synchronization and acceleration of the Na/K pumping rate in the cell membrane of skeletal muscle fibers by a specially designed synchronization modulation electric field. In these studies we either used cut fibers under a voltage clamp or intact fibers, but in the presence of ion channels blockers. A question remained as to whether the field-induced activation observed in the pump molecules could effectively increase the intracellular ionic concentration and the membrane potential at physiological conditions. In this paper, we studied the effects of the field on intact fibers without any channel blockers. We monitored the field-induced changes in the ionic concentration gradient across the cell membrane and the membrane potential non-invasively by using a fluorescent probe and confocal microscopic imaging techniques. The results clearly show that the entrainment of the pump molecules by the synchronization modulation electric field can effectively increase the ionic concentration gradient, and hence, hyperpolarize the membrane potential.
Chen
Wei
W
Cellular and Molecular Biophysics, University of South Florida, Tampa, FL 33620, USA. wchen@cas.usf.edu
Dando
Robin
R
D000818:Animals; D002462:Cell Membrane / Q000502:physiology; D004558:Electric Stimulation; D004574:Electromagnetic Fields; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000502:physiology*; D009119:Muscle Contraction / Q000502:physiology; D018485:Muscle Fibers, Skeletal / Q000502:physiology*; D018482:Muscle, Skeletal / Q000502:physiology*; D009942:Organometallic Compounds / Q000737:chemistry; D011894:Rana pipiens / Q000502:physiology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000502:physiology*
D009942:Organometallic Compounds; C110932:tetramethyl rhodamine ethyl ester; D000254:Sodium-Potassium-Exchanging ATPase