title: Suppression by Curcuma comosa Roxb. of pro-inflammatory cytokine secretion in phorbol-12-myristate-13-acetate stimulated human mononuclear cells.
abstract: Curcuma comosa Roxb. is a medicinal plant that has traditionally been used in Thailand for treatment of inflammation in postpartum uterine bleeding. The purpose of this study was to evaluate its anti-inflammatory effects using peripheral blood mononuclear cells (PBMC) and human pro-monocytic cell line (U937). Pretreatment with hexane or ethanol extract or two diarylhepatanoids (5-hydroxy-7-(4-hydroxyphenyl)-1-phenyl-(1E)-1-heptene and 7-(3,4-dihydroxyphenyl)-5-hydroxy-1-phenyl-(1E)-1-heptene) of C. comosa significantly decreased the release of pro-inflammatory cytokines, tumor necrosis factor alpha (TNF-alpha) and interleukin-1beta, from phorbol-12-myristate-13-acetate (PMA)-stimulated PBMC and U937 cells. In PMA-stimulated U937 cells, the two C. comosa diarylhepatanoids reduced the expression of TNF-alpha and suppressed expression of IkappaB kinase and activation of nuclear factor kappa B. These results indicated that C. comosa and its diarylheptanoids have anti-inflammatory properties which could be exploited for clinical use.
authors.affiliation: Toxicology Graduate Program, Faculty of Science, Mahidol University, Bangkok, Thailand.
authors.forename: Amorntus
authors.initials: A
authors.lastname: Sodsai
authors.forename: Pawinee
authors.initials: P
authors.lastname: Piyachaturawat
authors.forename: Samaisukh
authors.initials: S
authors.lastname: Sophasan
authors.forename: Apichart
authors.initials: A
authors.lastname: Suksamrarn
authors.forename: Molvibha
authors.initials: M
authors.lastname: Vongsakul
mesh_terms: D000328:Adult; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D030024:Curcuma / Q000737:chemistry*; D036381:Diarylheptanoids / Q000494:pharmacology; D006801:Humans; D051550:I-kappa B Kinase / Q000037:antagonists & inhibitors / Q000378:metabolism; D053583:Interleukin-1beta / Q000037:antagonists & inhibitors / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000378:metabolism; D020298:U937 Cells
chemical_list: D000893:Anti-Inflammatory Agents; D036381:Diarylheptanoids; D053583:Interleukin-1beta; D016328:NF-kappa B; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D051550:I-kappa B Kinase; D013755:Tetradecanoylphorbol Acetate