Hydroxylation of the triterpenoid nigranoic acid by the fungus Gliocladium roseum YMF1.00133.
The ability of the fungus Gliocladium roseum YMF1.00133 to transform the bioactive nigranoic acid (=(24Z)-9,19-cyclo-3,4-secolanosta-4(28),24-diene-3,26-dioic acid) was investigated. Three new products from the co-cultures of nigranoic acid and G. roseum YMF1.00133 were obtained by employing a combination of Sephadex LH-20 and silica-gel column chromatography. The major metabolite was identified as 15beta-hydroxynigranoic acid, and the minor metabolites as 6alpha,15beta-dihydroxynigranoic acid and 7beta,15beta-dihydroxynigranoic acid by mass spectrometry and NMR spectroscopy. This is the first report of the biotransformation of the A-ring-secocycloartene triterpenoid, nigranoic acid.
Dong
Jin-Yan
JY
Key Laboratory for Conservation and Utilization of Bioresources, Yunnan University, Kunming, Yunnan 650091, People's Republic of China.
Chen
Ye-Gao
YG
Song
Hong-Chuan
HC
Zhu
Yan-Hui
YH
Zhou
Yong-Ping
YP
Li
Lei
L
He
Yan-Ping
YP
Cao
Jie
J
Zhang
Ke-Qin
KQ
D020462:Gliocladium / Q000378:metabolism*; D006900:Hydroxylation; D013058:Mass Spectrometry; D014315:Triterpenes / Q000737:chemistry / Q000378:metabolism*
D014315:Triterpenes; C100860:nigranoic acid