title: CD83 expression on dendritic cells and T cells: correlation with effective immune responses.
abstract: Human CD83 is a marker molecule for mature dendritic cells (DC) and is also expressed on activated B and T cells. Although CD83 has been implicated in immune responses, its function on DC and T cells remains unclear. In this study, we wanted to assess the role of CD83 expressed on DC and T cells in the immune response. Down-regulation of CD83 expression on human DC through RNA interference (RNAi) results in a less potent induction of allogeneic T cell proliferation, reduced IFN-gamma secretion by established T cells and decreased capacity in the priming of functional tumor antigen-specific CD8+ T lymphocytes. In addition, CD83 mRNA-electroporated DC are stronger T cell stimulators. However, CD83 overexpression on Melan-A/MART-1-specific tumor-infiltrating lymphocytes (TIL) circumvents the need for CD83 expression on DC. Co-culture of immature DC with TIL or K562 cells overexpressing CD83 results in the production of enhanced levels of pro-inflammatory cytokines, whereas this production is less pronounced or even absent in co-cultures with non-modified TIL or K562 cells. In conclusion, we demonstrate that CD83 expression on T cells and DC modulates the immune response by activating DC and by delivering costimulatory signals for the stimulation of naive and memory T cells, respectively.
authors.affiliation: Laboratory of Molecular and Cellular Therapy, Department of Physiology and Immunology, Medical School of the Vrije Universiteit Brussel, Brussels, Belgium.
authors.forename: Cindy
authors.initials: C
authors.lastname: Aerts-Toegaert
authors.forename: Carlo
authors.initials: C
authors.lastname: Heirman
authors.forename: Sandra
authors.initials: S
authors.lastname: Tuyaerts
authors.forename: Jurgen
authors.initials: J
authors.lastname: Corthals
authors.forename: Joeri L
authors.initials: JL
authors.lastname: Aerts
authors.forename: Aude
authors.initials: A
authors.lastname: Bonehill
authors.forename: Kris
authors.initials: K
authors.lastname: Thielemans
authors.forename: Karine
authors.initials: K
authors.lastname: Breckpot
mesh_terms: D015703:Antigens, CD / Q000096:biosynthesis* / Q000235:genetics*; D002460:Cell Line; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis* / Q000235:genetics*; D020014:K562 Cells; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics*; D015398:Signal Transduction / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D000097802:CD83 Antigen
chemical_list: D015703:Antigens, CD; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen