Deltamethrin induces mitochondrial membrane permeability and altered expression of cytochrome C in rat brain.
Deltamethrin is a type II synthetic pyrethroid insecticide and is used extensively. Numerous studies have demonstrated that deltamethrin can cause severe central nervous system symptoms. The mechanism of neuro-toxicity caused by deltamethrin is still poorly understood. This study investigated the effect of deltamethrin on the mitochondria-mediated apoptosis pathway in rat brain. The cortex and hippocampus mitochondrial membrane potential, permeability transition, expression of cytochrome c and activity of cytochrome c oxidase was assayed at 5, 24, 48 and 72 h following deltamethrin treatment. The study observed that the membrane potential, change of absorbance at 540 nm (A(540)), intensities of cytochrome c in mitochondria and activity of cytochrome c oxidase in the cortex and hippocampus of treated groups were significantly lower than those of the control group. The results of the study demonstrated that deltamethrin induced a decreased mitochondrial membrane potential and increased permeability, and reduced the expression of cytochrome c, thus depressing the activity of cytochrome c oxidase significantly. It indicates that deltamethrin may have an effect on mitochondria-mediated apoptosis of nerve cells in the rat brain.
Chen
Dan
D
Department of Toxicology, MOE Key Laboratory of Environmental and Health, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, Hubei, China.
Huang
Xiaowei
X
Liu
Linlin
L
Shi
Nian
N
D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D045304:Cytochromes c / Q000096:biosynthesis*; D003576:Electron Transport Complex IV / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D007274:Injections, Intraperitoneal; D007306:Insecticides / Q000008:administration & dosage / Q000633:toxicity; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D033681:Mitochondrial Membrane Transport Proteins / Q000378:metabolism; D051336:Mitochondrial Membranes / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D009570:Nitriles / Q000008:administration & dosage / Q000633:toxicity*; D010539:Permeability / Q000187:drug effects; D011722:Pyrethrins / Q000008:administration & dosage / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D007306:Insecticides; D033681:Mitochondrial Membrane Transport Proteins; D009570:Nitriles; D011722:Pyrethrins; C017180:decamethrin; D045304:Cytochromes c; D003576:Electron Transport Complex IV