title: Altered K+ fluxes and insulin release in pancreatic islets from omega3 fatty acid-depleted rats.
abstract: A low intake of long-chain polyunsaturated omega3 fatty acid often prevails in Western populations. Its consequences in terms of the control of fuel homeostasis led us to explore functional events in pancreatic islets isolated from either normal or omega3-depleted rats (second generation). In the latter rats, the inflow of K+ by both ouabain-sensitive and ouabain-resistant modalities was decreased, this coinciding with an impaired insulin secretory response to ouabain. The intravenous injection of a medium-chain triglyceride:fish oil emulsion to omega3-depleted rats 2 h before sacrifice restored a normal value for the inflow of K+ by the ouabainsensitive modality, i.e., that linked to the activity of the Na,K-ATPase, but failed to correct the entry of K+ by the ouabain-resistant modality and the defect of the insulin secretory response to ouabain. In conclusion, an impaired activity of the Na,K-ATPase in insulin-producing cells apparently represents a key determinant of altered islet function in omega3-depleted rats.
authors.affiliation: Laboratorie of Experimental Hormonology, Brussels Free University, Brussels, Belgium.
authors.forename: Abdullah
authors.initials: A
authors.lastname: Sener
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Karim
authors.initials: K
authors.lastname: Louchami
authors.forename: Berrin
authors.initials: B
authors.lastname: Oguzhan
authors.forename: Philippe
authors.initials: P
authors.lastname: Courtois
authors.forename: Laurence
authors.initials: L
authors.lastname: Portois
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Chardigny
authors.forename: Yvon A
authors.initials: YA
authors.lastname: Carpentier
authors.forename: Willy J
authors.initials: WJ
authors.lastname: Malaisse
mesh_terms: D000818:Animals; D003571:Cytochalasin B / Q000494:pharmacology; D015525:Fatty Acids, Omega-3 / Q000502:physiology*; D005260:Female; D005395:Fish Oils / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D017136:Ion Transport; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D007328:Insulin; D003571:Cytochalasin B; D010042:Ouabain; D005947:Glucose; D013755:Tetradecanoylphorbol Acetate; D011188:Potassium