Impact of competitive inhibition and sequence variation upon the sensitivity of malaria PCR.
Real-time PCR assays for Plasmodium species utilizing universal and species-specific primers were compared to investigate variables influencing decreased assay sensitivity. Sequence variation in oligomer targets and competitive inhibition of dual-species templates in universal-primer mixes were found to decrease assay sensitivity.
Bialasiewicz
Seweryn
S
Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Building C28, Back Road, Royal Children's Hospital & Health Service District, Herston, Queensland, Australia 4029. seweryn@uq.edu.au
Whiley
David M
DM
Nissen
Michael D
MD
Sloots
Theo P
TP
D000818:Animals; D016054:DNA, Protozoan / Q000235:genetics; D005188:False Negative Reactions; D014644:Genetic Variation; D016778:Malaria, Falciparum / Q000175:diagnosis* / Q000469:parasitology; D016780:Malaria, Vivax / Q000175:diagnosis* / Q000469:parasitology; D010961:Plasmodium / Q000235:genetics* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D016053:RNA, Protozoan / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012680:Sensitivity and Specificity; D013045:Species Specificity
D016054:DNA, Protozoan; D016053:RNA, Protozoan; D012337:RNA, Ribosomal, 18S