title: The GCR1, GPA1, PRN1, NF-Y signal chain mediates both blue light and abscisic acid responses in Arabidopsis.
abstract: Different classes of biotic (e.g. plant hormones) and abiotic (e.g. different wavelengths of light) signals act through specific signal transduction mechanisms to coordinate higher plant development. While a great deal of progress has been made, full signal transduction chains have not yet been described for most blue light- or abscisic acid-mediated events. Based on data derived from T-DNA insertion mutants and yeast (Saccharomyces cerevisiae) two-hybrid and coprecipitation assays, we report a signal transduction chain shared by blue light and abscisic acid leading to light-harvesting chlorophyll a/b-binding protein expression in etiolated Arabidopsis (Arabidopsis thaliana) seedlings. The chain consists of GCR1 (the sole Arabidopsis protein coding for a potential G-protein-coupled receptor), GPA1 (the sole Arabidopsis Galpha-subunit), Pirin1 (PRN1; one of four members of an iron-containing subgroup of the cupin superfamily), and a nuclear factor Y heterotrimer comprised of A5, B9, and possibly C9. We also demonstrate that this mechanism is present in imbibed seeds wherein it affects germination rate.
authors.affiliation: Laboratory for Molecular Biology, Department of Biological Sciences , University of Illinois, Chicago, Illinois 60607, USA.
authors.forename: Katherine M
authors.initials: KM
authors.lastname: Warpeha
authors.forename: Snehali
authors.initials: S
authors.lastname: Upadhyay
authors.forename: Jennifer
authors.initials: J
authors.lastname: Yeh
authors.forename: Julia
authors.initials: J
authors.lastname: Adamiak
authors.forename: Samuel I
authors.initials: SI
authors.lastname: Hawkins
authors.forename: Yevgeniya R
authors.initials: YR
authors.lastname: Lapik
authors.forename: Mary Beth
authors.initials: MB
authors.lastname: Anderson
authors.forename: Lon S
authors.initials: LS
authors.lastname: Kaufman
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D017360:Arabidopsis / Q000196:embryology / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D018525:Germination; D008027:Light; D012639:Seeds / Q000254:growth & development; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques
chemical_list: D029681:Arabidopsis Proteins; D000040:Abscisic Acid