Bradykinin-related peptides, including a novel structural variant, (Val1)-bradykinin, from the skin secretion of Guenther's frog, Hylarana guentheri and their molecular precursors.
Multiple bradykinin-related peptides including a novel bradykinin structural variant, (Val(1))-bradykinin, have been identified from the defensive skin secretion of Guenther's frog, Hylarana guentheri by a tandem mass spectrometry method. Subsequently, four different preprobradykinin cDNAs, which encoded multiple bradykinin copies and its structural variants, were consistently cloned from a skin derived cDNA library. These preprobradykinin cDNAs showed little structural similarity with mammalian kininogens and the kininogens from the skin of toads, but have regions that are highly conserved in the kininogens from another ranid frog, Odorrana schmackeri. Alignment of these preprobradykinins revealed that preprobradykinin 1, 2 and 3 may derive from a single gene by alternative exon splicing.
Zhou
Jianwu
J
Institute of Biomedical Sciences, University of Ulster, Cromore Road, Coleraine BT52 1SA, Northern Ireland, UK.
Bjourson
Anthony J
AJ
Coulter
Daniel J M
DJ
Chen
Tianbao
T
Shaw
Chris
C
O'rourke
Martin
M
Hirst
David G
DG
Zhang
Yany
Y
Rao
Pingfan
P
McClean
Stephen
S
D000595:Amino Acid Sequence; D029845:Amphibian Proteins / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D001001:Anura / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001920:Bradykinin / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D007704:Kininogens / Q000737:chemistry; D008297:Male; D008969:Molecular Sequence Data; D009130:Muscle, Smooth / Q000187:drug effects; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D011498:Protein Precursors / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D017208:Rats, Wistar; D017422:Sequence Analysis, DNA; D012867:Skin / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
D029845:Amphibian Proteins; D018076:DNA, Complementary; D007704:Kininogens; D010455:Peptides; D011498:Protein Precursors; D001920:Bradykinin