Structural malleability of plasticins: preorganized conformations in solution and relevance for antimicrobial activity.
Plasticins (23 long-residue glycine-leucine-rich dermaseptin-related peptides produced by the skin of South American hylids) have very similar amino acid sequences, hydrophobicities, and amphipathicities, but differ in their membrane-damaging properties and structurations (i.e. destabilized helix states, beta-hairpin, beta-sheet, and disordered states) at anionic and zwitterionic membrane interfaces. Structural malleability of plasticins in aqueous solutions together with parameters that may govern their ability to fold within beta-hairpin like structures were analyzed through circular dichroism and FTIR spectroscopic studies completed by molecular dynamics simulations in polar mimetic media. The goal of this study was to probe to which extent pre-existent peptide conformations, i.e. intrinsic "conformational landscape", may be responsible for variability in bioactive conformation and antimicrobial/hemolytic mechanisms of action of these peptides in relation with their various membrane disturbing properties. All plasticins present a turn region that does not always result in folding into a beta-hairpin shaped conformation. Residue at position 8 plays a major role in initiating the folding, while position 12 is not critical. Conformational stability has no major impact on antimicrobial efficacy. However, preformed beta-hairpin in solution may act as a conformational lock that prevents switch to alpha-helical structure. This lock lowers the antimicrobial efficiency and explains subtle differences in potencies of the most active antimicrobial plasticins.
Bruston
F
F
FRE 2852 Protéines: Biochimie Structurale et Fonctionnelle, Université Paris 6-CNRS, Peptidome de la peau d'amphibiens, tour 43, 4, Place Jussieu 75252 Paris Cedex 05, France.
Lacombe
C
C
Zimmermann
K
K
Piesse
C
C
Nicolas
P
P
El Amri
C
C
D029845:Amphibian Proteins / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry* / Q000302:isolation & purification; D001001:Anura; D002942:Circular Dichroism; D003198:Computer Simulation; D006860:Hydrogen Bonding; D015394:Molecular Structure; D011487:Protein Conformation; D011489:Protein Denaturation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D012867:Skin / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013057:Spectrum Analysis
D029845:Amphibian Proteins; D023181:Antimicrobial Cationic Peptides