title: [Microbiology and clinical analysis of six cases of hospital-acquired pneumonia caused by Acinetobacter baumannii].
abstract: OBJECTIVE
  To study the microbiology, clinical features and treatment outcomes of hospital-acquired pneumonia (HAP) caused by Acinetobacter baumannii.
  
  
  METHODS
  Six cases of HAP, caused by Acinetobacter baumannii producing PER-1 type extended-spectrum beta-lactamase verified by molecular biological methods, were studied in Bethune International Peace Hospital, from January to August 2005. Clinical data were collected and analyzed. Pulsed-field gel electrophoresis (PFGE) were used to analyze the homology of these strains. The genes of beta-lactamase (BLA), aminoglycoside-modifying enzymes (AME), resistant to disinfectant-sulfanilamide (qacEDelta1-sul1), and class 1 integrase (intl1) were analyzed using PCR and verified by DNA sequencing.
  
  
  RESULTS
  Among these 6 strains of Acinetobacter baumannii, five were susceptible only to imipenem, and 4 were susceptible to meropenem. The PFGE types identified from these isolates were A1 (n=2) and A2-A5 (n=1, respectively). They all were positive for PER-1, TEM-1, and genes of qacEDelta1-sull and intl1. Three of them harbored genes of aac (3)-I and aac (6')-I. Two had aac (3)-I and ant (3'')-I. One had aac (3)-I. All the patients had severe underlying diseases, and had received mechanical ventilation. They all had received broad spectrum antibiotics within 15 days before Acinetobacter baumannii were identified. Although carbapenem and/or cefoperazone/sulbactam had been used, only 3 patients survived.
  
  
  CONCLUSIONS
  The 6 PER-1 type extended-spectrum beta-lactamase-producing Acinetobacter baumannii isolates were closely related and had complex mechanisms of drug resistance. The prognosis of patients infected by them was poor.
authors.affiliation: Department of Laboratory of Bethune International Peace Hospital, Shijiazhuang 050082, China.
authors.forename: Tian-wen
authors.initials: TW
authors.lastname: Hou
authors.forename: Xiao-lin
authors.initials: XL
authors.lastname: Yin
authors.forename: Chao-ying
authors.initials: CY
authors.lastname: Jiang
authors.forename: Zhong-hua
authors.initials: ZH
authors.lastname: Wang
authors.forename: Qing-kui
authors.initials: QK
authors.lastname: Chen
authors.forename: Xing
authors.initials: X
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Yun
authors.initials: Y
authors.lastname: Bai
mesh_terms: D000151:Acinetobacter Infections / Q000382:microbiology*; D040981:Acinetobacter baumannii / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003428:Cross Infection / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018410:Pneumonia, Bacterial / Q000382:microbiology*; D001618:beta-Lactamases / Q000096:biosynthesis
chemical_list: D004269:DNA, Bacterial; D001618:beta-Lactamases