title: Cytotoxicity of submicron emulsions and solid lipid nanoparticles for dermal application.
abstract: The cytotoxicity and physical properties of various submicron O/W emulsions and solid lipid nanoparticles for dermal applications were investigated. Droplet size and zetapotential of submicron emulsions depended on the composition of the cosurfactant blend used. The viability of J774 macrophages, mouse 3T3 fibroblasts and HaCaT keratinocytes was significantly reduced in the presence of stearylamine. Nanoparticles consisting of stearic acid or different kinds of adeps solidus could be manufactured when formulated with lecithin, sodium taurocholate, polysorbate 80 and stearylamine. Survival of macrophages was highly affected by stearic acid and stearylamine. In general a viability of more than 90% was observed when semi-synthetic glycerides or hard fat was employed to formulate nanoparticles.
authors.affiliation: Laboratory of Pharmaceutical Technology and Biopharmacy, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium.
authors.forename: Wim
authors.initials: W
authors.lastname: Weyenberg
authors.forename: Plamen
authors.initials: P
authors.lastname: Filev
authors.forename: Dave
authors.initials: D
authors.lastname: Van den Plas
authors.forename: Jo
authors.initials: J
authors.lastname: Vandervoort
authors.forename: Kris
authors.initials: K
authors.lastname: De Smet
authors.forename: Philippe
authors.initials: P
authors.lastname: Sollie
authors.forename: Annick
authors.initials: A
authors.lastname: Ludwig
mesh_terms: D000279:Administration, Cutaneous; D000588:Amines / Q000633:toxicity; D000818:Animals; D041702:BALB 3T3 Cells; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers; D004655:Emulsions; D005223:Fats / Q000633:toxicity; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects; D008055:Lipids / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D053758:Nanoparticles; D010316:Particle Size; D010713:Phosphatidylcholines / Q000633:toxicity; D010718:Phosphatidylserines / Q000633:toxicity; D011136:Polysorbates / Q000633:toxicity; D013024:Soybean Oil / Q000633:toxicity; D013229:Stearic Acids / Q000633:toxicity; D013501:Surface-Active Agents / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D013656:Taurocholic Acid / Q000633:toxicity; D014867:Water / Q000737:chemistry
chemical_list: D000588:Amines; D004337:Drug Carriers; D004655:Emulsions; D005223:Fats; D008055:Lipids; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; D011136:Polysorbates; D013229:Stearic Acids; D013501:Surface-Active Agents; D014867:Water; C031183:stearic acid; D013656:Taurocholic Acid; D013024:Soybean Oil; C009317:stearylamine