A dynamic model of gene expression in monocytes reveals differences in immediate/early response genes between adult and neonatal cells.
Neonatal monocytes display immaturity of numerous functions compared with adult cells. Gene expression arrays provide a promising tool for elucidating mechanisms underlying neonatal immune function. We used a well-established microarray to analyze differences between LPS-stimulated human cord blood and adult monocytes to create dynamic models for interactions to elucidate observed deficiencies in neonatal immune responses. We identified 168 genes that were differentially expressed between adult and cord monocytes after 45 min incubation with LPS. Of these genes, 95% (159 of 167) were over-expressed in adult relative to cord monocytes. Differentially expressed genes could be sorted into nine groups according to their kinetics of activation. Functional modelling suggested differences between adult and cord blood in the regulation of apoptosis, a finding confirmed using annexin binding assays. We conclude that kinetic studies of gene expression reveal potentially important differences in gene expression dynamics that may provide insight into neonatal innate immunity.
Lawrence
Shelley
S
Dept. of Pediatrics, Neonatal Section, University of Oklahoma College of Medicine, Oklahoma City, OK, USA.
Tang
Yuhong
Y
Arthritis & Immunology Program Oklahoma Medical Research Foundation, Oklahoma City, 73104, USA.
Frank
M Barton
MB
Arthritis & Immunology Program Oklahoma Medical Research Foundation, Oklahoma City, 73104, USA.
Dozmorov
Igor
I
Arthritis & Immunology Program Oklahoma Medical Research Foundation, Oklahoma City, 73104, USA.
Jiang
Kaiyu
K
Dept. of Pediatrics, Neonatal Section, University of Oklahoma College of Medicine, Oklahoma City, OK, USA.
Chen
Yanmin
Y
Dept. of Pediatrics, Neonatal Section, University of Oklahoma College of Medicine, Oklahoma City, OK, USA.
Cadwell
Craig
C
Arthritis & Immunology Program Oklahoma Medical Research Foundation, Oklahoma City, 73104, USA.
Turner
Sean
S
Arthritis & Immunology Program Oklahoma Medical Research Foundation, Oklahoma City, 73104, USA.
Centola
Michael
M
Arthritis & Immunology Program Oklahoma Medical Research Foundation, Oklahoma City, 73104, USA.
Jarvis
James N
JN
Dept. of Pediatrics, Neonatal Section, University of Oklahoma College of Medicine, Oklahoma City, OK, USA.