Alterations of tumour suppressor gene PPP2R1B in hepatocellular carcinoma.
To evaluate whether the tumour suppressor gene, PPP2R1B, is involved in pathogenesis of hepatocellular carcinoma (HCC), reverse transcription-polymerase chain reaction (RT-PCR) and cDNA sequencing were performed. Eleven of 38 (29%) tumours and 1 of 34 (3%) corresponding non-tumour tissues showed coexpression of wild-type and aberrant mRNA. Various deletions were found in aberrant transcripts. Southern blot analysis did not show gene deletion in tumours, suggesting abnormal RNA splicing may be involved. These data suggest the possibility that aberrant transcripts of PPP2R1B might be associated with the development of HCC.
Chou
Huei-Chi
HC
Department of Internal Medicine, National, Taiwan University Hospital and National Taiwan University College of Medicine, 7 Chung-Shan South Road, Taipei 10016, Taiwan.
Chen
Chien-Hung
CH
Lee
Hsuan-Shu
HS
Lee
Cha-Ze
CZ
Huang
Guan-Tarn
GT
Yang
Pei-Ming
PM
Lee
Po-Huang
PH
Sheu
Jin-Chuan
JC
D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D005260:Female; D017353:Gene Deletion; D016147:Genes, Tumor Suppressor; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics*; D054648:Protein Phosphatase 2; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D009363:Neoplasm Proteins; C432785:PPP2R1B protein, human; D054648:Protein Phosphatase 2