title: The role of calcium utilization of intestinal flora in urinary calcium excretion.
abstract: OBJECTIVE
  We aimed to evaluate whether Ca(+2) utilization of intestinal flora (IF) has an effect on urinary excretion of Ca(+2) (UCaE) levels.
  
  
  METHODS
  Fecal samples (0.1 g/mL) of children who underwent UCaE examination in the past year were implanted in broths. Labeled (45)Ca (5 muL) was added to the samples and incubated. From these samples, a 200-muL quantity was filtrated with a 0.45-micrometer membrane and was rinsed in 200 muL pure water. (45)Ca activity in the membrane was measured and defined as percent activity per bacteria ((45)Ca(act) %/CFU). Levels of aerobic and anaerobic (45)Ca(act) %/CFU and their correlations with UCaE were compared between hypercalciuric (Group I) and normocalciuric (Group II) patients.
  
  
  RESULTS
  Levels of (45)Ca %/CFU were similar between groups (P > .05). Aerobic and anaerobic (45)Ca(act)%/CFU levels were not significantly correlated to UCaE, either in normocalciuric (P = .079, r = -0.503; P = .260, r = -0.420, stray mart respectively) or in hypercalciuric children (P = .509, r = 0.223; P = .623, r = -0.257, respectively).
  
  
  CONCLUSIONS
  Similar (45)Ca(act)%/CFU levels in the 2 groups imply that calcium utilization of IF does not have a distinct effect on UCaE.
authors.affiliation: Department of Nuclear Applications, Ege University Institute of Nuclear Sciences, Izmir, Turkey.
authors.forename: Fatma Yurt
authors.initials: FY
authors.lastname: Lambrecht
authors.forename: Salih
authors.initials: S
authors.lastname: Kavukçu
authors.forename: Belde
authors.initials: B
authors.lastname: Kasap
authors.forename: Alper
authors.initials: A
authors.lastname: Soylu
authors.forename: Mine
authors.initials: M
authors.lastname: Yücesoy
authors.forename: Mehmet
authors.initials: M
authors.lastname: Türkmen
authors.forename: Nuran
authors.initials: N
authors.lastname: Esen
authors.forename: Perihan
authors.initials: P
authors.lastname: Unak
mesh_terms: D000293:Adolescent; D002118:Calcium / Q000378:metabolism* / Q000652:urine; D002648:Child; D002675:Child, Preschool; D005243:Feces / Q000382:microbiology; D005260:Female; D006801:Humans; D053565:Hypercalciuria / Q000378:metabolism* / Q000382:microbiology; D007223:Infant; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000382:microbiology*; D008297:Male
chemical_list: D002118:Calcium