title: The Notch-1 intracellular domain is found in sub-nuclear bodies in SH-SY5Y neuroblastomas and in primary cortical neurons.
abstract: Notch signalling affects most aspects of development, not least the determination of neural stem cell fate. Here, we describe the presence of the Notch-1 intracellular domain (N1(ICD)) in sub-nuclear bodies in SH-SY5Y neuroblastomas and in primary rat cortical neurons as well as several other mammalian cell lines. We also demonstrate that these N1(ICD)-positive sub-nuclear bodies are distinct from premyelocytic leukaemia (PML) and SC35 bodies. Furthermore, using Notch deletion constructs we determined that a region C-terminal of amino acid 2094 is involved in targeting the N1(ICD) into sub-nuclear bodies. These findings have ramifications for nuclear architecture and gene transcription.
authors.affiliation: King's College London, MRC Centre for Neurodegenerative Research, Institute of Psychiatry, De Crespigny Park, Denmark Hill, London SE5 8AF, UK.
authors.forename: Claudie
authors.initials: C
authors.lastname: Hooper
authors.forename: J Paul
authors.initials: JP
authors.lastname: Chapple
authors.forename: Simon
authors.initials: S
authors.lastname: Lovestone
authors.forename: Richard
authors.initials: R
authors.lastname: Killick
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology*; D006224:Cricetinae; D003412:Cricetulus; D004622:Embryo, Mammalian; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D016296:Mutagenesis / Q000502:physiology; D009363:Neoplasm Proteins / Q000378:metabolism; D009447:Neuroblastoma / Q000473:pathology*; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure; D009687:Nuclear Proteins / Q000378:metabolism; D000071156:Promyelocytic Leukemia Protein; D017434:Protein Structure, Tertiary / Q000502:physiology; D051381:Rats; D051881:Receptor, Notch1 / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism; D014162:Transfection / Q000379:methods; D025521:Tumor Suppressor Proteins / Q000378:metabolism
chemical_list: C497761:NOTCH1 protein, human; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D000071156:Promyelocytic Leukemia Protein; D051881:Receptor, Notch1; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; C070576:PML protein, human; D049452:Green Fluorescent Proteins