title: Presynaptic GABAA receptors facilitate GABAergic transmission to dopaminergic neurons in the ventral tegmental area of young rats.
abstract: Gamma-aminobutyric acid A receptor (GABA(A)R)-mediated postsynaptic currents (IPSCs) were recorded from dopaminergic neurons of the ventral tegmental area of young rats in acute brain slices and from mechanically dissociated neurons. Low concentrations (0.1-0.3 microm) of muscimol, a selective GABA(A)R agonist, increased the amplitude, and reduced the paired pulse ratio of evoked IPSCs. Moreover, muscimol increased the frequency but not the amplitude of spontaneous IPSCs (sIPSCs). These data point to a presynaptic locus of muscimol action. It is interesting that 1 microm muscimol caused an inhibition of sIPSCs, which was reversed to potentiation by the GABA(B) receptor antagonist CGP52432. Isoguvacine, a selective GABA(A)R agonist that belongs to a different class, mimicked the effects of muscimol on sIPSCs: it increased them at low (<or= 0.5 microm), and decreased them at a higher concentration (1 microm). Hence, the activation of presynaptic GABA(A)Rs facilitates GABA release, which is limited by presynaptic GABA(B)Rs. Furthermore, facilitation of sIPSCs by muscimol was eliminated in a medium containing tetrodotoxin or cadmium. It is noteworthy that sIPSC frequency was greatly increased by 4,5,6,7-tetrahydroisoxazolo[5,4-c]pyridin-3-ol(gaboxadol, or THIP), an agonist with preferential effects on extrasynaptic GABA(A)Rs containing alpha4betadelta subunits, or by guvacine, a GABA transport blocker, which increases ambient GABA levels. In addition, sIPSC frequency was attenuated by furosemide, a selective antagonist of alpha6 subunits. Thus, the presynaptic GABA(A)Rs may be situated at extrasynaptic sites and may contain alpha4/6betadelta subunits. Given the marked sensitivity of extrasynaptic GABA(A)Rs to ambient GABA, alcohols and anaesthetics, these receptors may present a critical site for regulating synaptic function in the developing brain in both physiological and pathological situations.
authors.affiliation: Department of Anesthesiology, Pharmacology and Physiology, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, 185 South Orange Avenue, Newark, NJ 07103, USA.
authors.forename: Cheng
authors.initials: C
authors.lastname: Xiao
authors.forename: Chunyi
authors.initials: C
authors.lastname: Zhou
authors.forename: Keyong
authors.initials: K
authors.lastname: Li
authors.forename: Jiang-Hong
authors.initials: JH
authors.lastname: Ye
mesh_terms: D000818:Animals; D002104:Cadmium / Q000494:pharmacology; D004298:Dopamine / Q000378:metabolism*; D018755:GABA Agonists / Q000494:pharmacology; D066298:In Vitro Techniques; D053444:Inhibitory Postsynaptic Potentials / Q000187:drug effects; D009118:Muscimol / Q000494:pharmacology; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D017729:Presynaptic Terminals / Q000378:metabolism*; D020033:Protein Isoforms / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000502:physiology*; D009435:Synaptic Transmission / Q000502:physiology*; D013779:Tetrodotoxin / Q000494:pharmacology; D017557:Ventral Tegmental Area / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
chemical_list: D018755:GABA Agonists; D020033:Protein Isoforms; D011963:Receptors, GABA-A; D002104:Cadmium; D009118:Muscimol; D013779:Tetrodotoxin; D005680:gamma-Aminobutyric Acid; D004298:Dopamine