title: Some fungal endophytes from vegetable crops and their anti-oomycete activities against tomato late blight.
abstract: OBJECTIVE
  To isolate endophytic fungi from vegetable plants and examine their in vivo anti-oomycete activity against Phytophthora infestans in tomato plants.
  
  
  RESULTS
  Endophytic fungi were isolated from surface-sterilized plant tissues and anti-oomycete activity was measured by in vivo assay using tomato seedlings. Endophytic fungi showing potent anti-oomycete activity were identified by morphological characteristics and nuclear ribosomal ITS1-5.8S-ITS2 sequence analysis. A total of 152 isolates were obtained from 66 healthy tissue samples of cucumber, red pepper, tomato, pumpkin and Chinese cabbage and the fermentation broths of 23 isolates showed potent in vivo anti-oomycete activity against tomato late blight with control values over 90%. The Fusarium oxysporum strain EF119, which was isolated from roots of red pepper, showed the most potent disease control efficacy against tomato late blight. In dual-culture tests, it inhibited the growth of Pythium ultimum, P. infestans and Phytophthora capsici.
  
  
  CONCLUSIONS
  Among endophytic fungi isolated from healthy tissues of vegetable plants, F. oxysporum EF119 showed the most potent in vivo anti-oomycete activity against tomato late blight and in vitro anti-oomycete activity against several oomycete pathogens.
  
  
  CONCLUSIONS
  Endophytic fungi showing anti-oomycete activity in vitro and in vivo may be used as biocontrol agents particularly of tomato late blight.
authors.affiliation: Biological Function Research Team, Korea Research Institute of Chemical Technology, Taejon, Korea.
authors.forename: H-Y
authors.initials: HY
authors.lastname: Kim
authors.forename: G J
authors.initials: GJ
authors.lastname: Choi
authors.forename: H B
authors.initials: HB
authors.lastname: Lee
authors.forename: S-W
authors.initials: SW
authors.lastname: Lee
authors.forename: H K
authors.initials: HK
authors.lastname: Lim
authors.forename: K S
authors.initials: KS
authors.lastname: Jang
authors.forename: S W
authors.initials: SW
authors.lastname: Son
authors.forename: S O
authors.initials: SO
authors.lastname: Lee
authors.forename: K Y
authors.initials: KY
authors.lastname: Cho
authors.forename: N D
authors.initials: ND
authors.lastname: Sung
authors.forename: J-C
authors.initials: JC
authors.lastname: Kim
mesh_terms: D018556:Crops, Agricultural / Q000382:microbiology*; D005658:Fungi / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology*; D018551:Solanum lycopersicum / Q000382:microbiology*; D009868:Oomycetes / Q000502:physiology*; D010572:Pest Control, Biological; D010935:Plant Diseases / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology; D014675:Vegetables / Q000382:microbiology*