title: An evaluation of GC-MS and HPLC-FD methods for analysis of protein binders in paintings.
abstract: Two chromatographic methods have been compared for analysis of protein-binding media used in paintings, namely, HPLC with fluorescence detection and GC-MS. The proteins were hydrolyzed to the corresponding amino acids (AAs) by gaseous HCl and the AAs were derivatized with methyl chloroformate, followed by GC-MS or by HPLC after derivatization with the AccQ fluorescence reagent. The hydrolysis, derivatization reactions and the chromatographic procedures have been optimized and applied to standard binding media, model and real samples of paintings. The methods have been compared and critically evaluated.
authors.affiliation: Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Albertov, Prague, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Prikryl
authors.forename: Lenka
authors.initials: L
authors.lastname: Havlícková
authors.forename: Vĕra
authors.initials: V
authors.lastname: Pacáková
authors.forename: Janka
authors.initials: J
authors.lastname: Hradilová
authors.forename: Karel
authors.initials: K
authors.lastname: Stulík
authors.forename: Petr
authors.initials: P
authors.lastname: Hofta
mesh_terms: D001843:Bone Cements / Q000032:analysis / Q000737:chemistry; D002364:Caseins / Q000032:analysis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004527:Egg Proteins / Q000032:analysis / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D010150:Paint; D010151:Paintings; D011485:Protein Binding; D011506:Proteins / Q000032:analysis* / Q000737:chemistry
chemical_list: D001843:Bone Cements; D002364:Caseins; D004527:Egg Proteins; D011506:Proteins