title: Hydroxylation of the triterpenoid nigranoic acid by the fungus Gliocladium roseum YMF1.00133.
abstract: The ability of the fungus Gliocladium roseum YMF1.00133 to transform the bioactive nigranoic acid (=(24Z)-9,19-cyclo-3,4-secolanosta-4(28),24-diene-3,26-dioic acid) was investigated. Three new products from the co-cultures of nigranoic acid and G. roseum YMF1.00133 were obtained by employing a combination of Sephadex LH-20 and silica-gel column chromatography. The major metabolite was identified as 15beta-hydroxynigranoic acid, and the minor metabolites as 6alpha,15beta-dihydroxynigranoic acid and 7beta,15beta-dihydroxynigranoic acid by mass spectrometry and NMR spectroscopy. This is the first report of the biotransformation of the A-ring-secocycloartene triterpenoid, nigranoic acid.
authors.affiliation: Key Laboratory for Conservation and Utilization of Bioresources, Yunnan University, Kunming, Yunnan 650091, People's Republic of China.
authors.forename: Jin-Yan
authors.initials: JY
authors.lastname: Dong
authors.forename: Ye-Gao
authors.initials: YG
authors.lastname: Chen
authors.forename: Hong-Chuan
authors.initials: HC
authors.lastname: Song
authors.forename: Yan-Hui
authors.initials: YH
authors.lastname: Zhu
authors.forename: Yong-Ping
authors.initials: YP
authors.lastname: Zhou
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: He
authors.forename: Jie
authors.initials: J
authors.lastname: Cao
authors.forename: Ke-Qin
authors.initials: KQ
authors.lastname: Zhang
mesh_terms: D020462:Gliocladium / Q000378:metabolism*; D006900:Hydroxylation; D013058:Mass Spectrometry; D014315:Triterpenes / Q000737:chemistry / Q000378:metabolism*
chemical_list: D014315:Triterpenes; C100860:nigranoic acid