Differentiation of wine lactic acid bacteria species based on RFLP analysis of a partial sequence of rpoB gene.
PCR-RFLP analysis of rpoB sequences were used to identify lactic acid bacteria (LAB) species commonly isolated from wine. Strains of seven cocci and 12 lactobacilli species could be identified after single digestion with AciI for the cocci and two or three digestions (AciI, HinfI and MseI) for the rods and preceded by colonies isolation on solid selective medium and microscope observation to distinguish cocci and rods cells.
Claisse
Olivier
O
UMR oenologie, ISVV INRA-Université Victor Segalen Bordeaux2, 351 cours de la libération, 33405 Talence cedex, France.
Renouf
Vincent
V
Lonvaud-Funel
Aline
A
D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005798:Genes, Bacterial; D007777:Lactobacillaceae / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D014920:Wine / Q000382:microbiology*
D004269:DNA, Bacterial; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; C066171:endodeoxyribonuclease AciI; C055927:endodeoxyribonuclease MseI; D015252:Deoxyribonucleases, Type II Site-Specific; C032229:GANTC-specific type II deoxyribonucleases