Shielding parenteral multivitamins from light increases vitamin A and E concentration in lung of newborn guinea pigs.
OBJECTIVE
Exposure of parenteral multivitamin preparation (MVP) to light generates peroxides. Light-exposed MVP induces an oxidant stress in lung but not in liver. This discrepancy suggests differences in handling of infused antioxidant vitamins between the two organs.


OBJECTIVE
antioxidant capacity of lung depends on the MVP concentration and light protection of infused solutions.


METHODS
Protocol 1: four groups of three-day old guinea pigs received the base solution (5% dextrose + 0.45% NaCl) enriched with 0%, 1%, 2% and 3% MVP. Protocol 2: three further groups received the base solution + 2% MVP either light-exposed or light-protected or light-protected + 300 microM H2O2. After 4 days, lung and liver were sampled for vitamin determinations. Data were analyzed by ANOVA.


RESULTS
In lung, vitamins A-C-E reached a plateau with 1% MVP. In liver, vitamin A and E increased according to their concentration in solutions. Light exposure and added-H2O2 were associated with lower vitamin E in lung and liver. Retinol was higher in lung and lower in liver of animals receiving light-protected compared to light-exposed solutions.


CONCLUSIONS
Light protection of 1% MVP is a better way to improve the pulmonary oxidant-antioxidant balance than to increase MVP (>1%) in parenteral nutrition.
Lavoie
Jean-Claude
JC
Department of Paediatrics, CHU Sainte-Justine, University of Montréal, Montréal, Que., Canada H3T 1C5. jean-claude.lavoie@recherche-ste-justine.qc.ca
Chessex
Philippe
P
Rouleau
Thérèse
T
Tsopmo
Apollinaire
A
Friel
James
J
D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D000975:Antioxidants / Q000378:metabolism*; D001205:Ascorbic Acid / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004355:Drug Stability; D004356:Drug Storage; D006168:Guinea Pigs; D008027:Light / Q000009:adverse effects*; D015227:Lipid Peroxidation / Q000528:radiation effects; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism*; D010084:Oxidation-Reduction; D010288:Parenteral Nutrition; D010777:Photochemistry; D014801:Vitamin A / Q000378:metabolism*; D014810:Vitamin E / Q000378:metabolism*
D000975:Antioxidants; D014801:Vitamin A; D014810:Vitamin E; D001205:Ascorbic Acid