title: [Expression of phosphorylated NMDA receptor 1 in the cerebral cortex after NMDA microinjection in vivo].
abstract: OBJECTIVE
  The activation of N-methyl-D-aspartate(NMDA) receptors plays critical roles in the pathogenesis of diseases of the brain. This study aimed to examine the expression of phosphor-NR1 S897 in the cerebral cortex after NMDA microinjection in vivo.
  
  
  METHODS
  Forty seven-day-old Sprague-Dawley rats were randomly assigned into normal control and NMDA injection groups. The rats from the NMDA injection group were injected with 10 mmol of NMDA and were sacrificed 1 hr after injection. 2, 3, 5-triphenyltetrazolium chloride (TTC) and fluorescent immunohistochemical stainings were conducted and the fluorescence intensity OD value between the two groups was compared.
  
  
  RESULTS
  TTC staining from the two groups was normal. Expression of phosphor-NR1 S897 in the cerebral cortex of the ipsilateral hemisphere to injection in the NMDA injection group decreased significantly compared with the normal control group, with OD values of 0.366 +/- 0.087 vs 1.364 +/- 0.268 (P < 0.01).
  
  
  CONCLUSIONS
  NMDA microinjection, as a hypoxia-ischemia (HI) insult, significantly decreased the expression of phosphor-NR1 S897. This indicates the importance of the "HI-NMDA-phospho-NR1 S897 dephosphorylation-cell damage" pathway in HI brain damage.
authors.affiliation: Division of Neonatology, Third Xiangya Hospital of Central South University, Changsha 410013, China. heiming_yan@yahoo.com.cn
authors.forename: Ming-Yan
authors.initials: MY
authors.lastname: Hei
authors.forename: Mi-Li
authors.initials: ML
authors.lastname: Xiao
authors.forename: Shou-Jin
authors.initials: SJ
authors.lastname: Kuang
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D020925:Hypoxia-Ischemia, Brain / Q000378:metabolism; D008297:Male; D008845:Microinjections; D016202:N-Methylaspartate / Q000008:administration & dosage*; D010766:Phosphorylation; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000032:analysis / Q000187:drug effects*
chemical_list: C409466:NR1 NMDA receptor; D016194:Receptors, N-Methyl-D-Aspartate; D016202:N-Methylaspartate