title: Cell adhesion and mechanical properties of a flexible scaffold for cardiac tissue engineering.
abstract: Cardiac tissue engineering is focused on obtaining functional cardiomyocyte constructs to provide an alternative to cellular cardiomyoplasty. Mechanical stimuli have been shown to stimulate protein expression and the differentiation of mammalian cells from contractile tissues. Our aim was to obtain a flexible scaffold which could be used to apply mechanical forces during tissue regeneration. Poly(1,8-octanediol-co-citric acid) (POC) is an elastomer that can be processed into scaffolds for tissue engineering. We investigated the effect of modifying the porosity on the mechanical properties of the POC scaffolds. In addition, the effects of the storage method and strain rate on material integrity were assessed. The maximum elongation of POC porous films varied from 60% to 160% of their original length. A decrease in the porosity caused a rise in this elastic modulus. The attachment of HL-1 cardiomyocytes to POC was assessed on films coated with fibronectin, collagen and laminin. These extracellular matrix proteins promoted cell adhesion in a protein-type- and concentration-dependent manner. Therefore, POC scaffolds can be optimised to meet the mechanical and biological parameters needed for cardiac culture. This porous material has the potential to be used for cardiac tissue engineering as well as for other soft tissue applications.
authors.affiliation: Division of Drug Delivery and Tissue Engineering, School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, UK.
authors.forename: L A
authors.initials: LA
authors.lastname: Hidalgo-Bastida
authors.forename: J J A
authors.initials: JJ
authors.lastname: Barry
authors.forename: N M
authors.initials: NM
authors.lastname: Everitt
authors.forename: F R A J
authors.initials: FR
authors.lastname: Rose
authors.forename: L D
authors.initials: LD
authors.lastname: Buttery
authors.forename: I P
authors.initials: IP
authors.lastname: Hall
authors.forename: W C
authors.initials: WC
authors.lastname: Claycomb
authors.forename: K M
authors.initials: KM
authors.lastname: Shakesheff
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002460:Cell Line; D002951:Citrates / Q000737:chemistry / Q000378:metabolism*; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism*; D003094:Collagen / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D020724:Elastomers / Q000737:chemistry / Q000378:metabolism*; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D005353:Fibronectins / Q000737:chemistry / Q000378:metabolism; D007797:Laminin / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D032383:Myocytes, Cardiac / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D011108:Polymers / Q000737:chemistry / Q000378:metabolism*; D016062:Porosity; D023822:Tissue Engineering / Q000379:methods*; D014057:Tomography, X-Ray Computed
chemical_list: D002951:Citrates; D020099:Coated Materials, Biocompatible; D020724:Elastomers; D005353:Fibronectins; D007797:Laminin; D011108:Polymers; D003094:Collagen