Myelin protein zero is one of the components of the detergent-resistant membrane microdomain fraction prepared from rat pituitary.
Pituitary gland is a well-known endocrine tissue. The hypothalamo-neurohypophysial system, containing arginine vasopressin and oxytocin, shows a reversible morphological reorganization of both neurons and glial cells during chronic physiological stimulations. Since many signal transducing and cell adhesion molecules (CAMs) are recovered in membrane microdomain (MD) fractions, MDs are considered as signaling platforms of cells. In order to know the molecular background for these endocrine systems, we characterized MD-components derived from rat pituitary and found specific enrichment of several proteins in the fraction. One of them was identified as myelin protein zero (P0) with mass analysis and this result was further confirmed by a result that a specific antibody to this protein reacted to the authentic P0 protein in the myelin fraction of rat sciatic nerve. P0 is one of type-I transmembrane CAMs and a major structural component of mammalian peripheral nerve myelin. In mammals, expression of P0 has been considered to be restricted to peripheral nervous system. This result however indicates that P0 expresses more widely and its enrichment in the MD-fraction from rat pituitary suggests the participation in cell-cell communications.
Taguchi
Katsutoshi
K
Division of Bioinformation, Department of Biosystems Science, Graduate School of Science and Technology, Kobe-University, Rokkodai-cho 1-1, Nada-ku, Kobe 657-8501, Japan.
Kumanogoh
Haruko
H
Nakamura
Shun
S
Miyata
Seiji
S
Maekawa
Shohei
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D001127:Arginine Vasopressin / Q000378:metabolism; D007150:Immunohistochemistry; D021962:Membrane Microdomains / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D018993:Myelin P0 Protein / Q000096:biosynthesis* / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D010121:Oxytocin / Q000378:metabolism; D010902:Pituitary Gland / Q000737:chemistry / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000737:chemistry / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*
D000911:Antibodies, Monoclonal; D018993:Myelin P0 Protein; D001127:Arginine Vasopressin; D010121:Oxytocin