title: Identification of potential anti-photoageing algal compounds using an in-vitro model of photoageing.
abstract: Stress-induced premature senescence (SIPS) has been proposed as an in-vitro model for testing the long-term effects of stressful events and to find molecules/natural extracts that protect against such stress. Premature senescence of human skin diploid fibroblasts (HDFs) can be induced by repeated subcytotoxic exposure to UVB, with the appearance of so-called biomarkers of senescence such as growth arrest, senescence-associated beta-galactosidase activity, senescence-associated gene over-expression and the common 4977-bp mitochondrial DNA deletion. This model of UVB-induced premature senescence has been acknowledged as a robust in-vitro model in photoageing research. In this study, the potential anti-photoageing effects of a series of algal extracts were tested. The appearance of the biomarkers of UVB-induced premature senescence of HDFs was studied with or without algal extracts. One algal extract was shown to be particularly protective against UVB-induced SIPS. The results obtained here reinforce the notion that UVB-induced premature senescence of HDFs can be used to screen potential anti-photoageing compounds.
authors.affiliation: Laboratory of Biochemistry and Cellular Biology, Department of Biology, University of Namur (FUNDP), Belgium.
authors.forename: F
authors.initials: F
authors.lastname: Debacq-Chainiaux
authors.forename: C
authors.initials: C
authors.lastname: Borlon
authors.forename: B
authors.initials: B
authors.lastname: De Hertogh
authors.forename: J
authors.initials: J
authors.lastname: Remacle
authors.forename: P-Y
authors.initials: PY
authors.lastname: Morvan
authors.forename: R
authors.initials: R
authors.lastname: Vallée
authors.forename: O
authors.initials: O
authors.lastname: Toussaint
mesh_terms: D002470:Cell Survival / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects; D051152:Clusterin / Q000235:genetics / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000235:genetics; D017484:Dermatitis, Phototoxic / Q000517:prevention & control; D056890:Eukaryota / Q000737:chemistry*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D008938:Mitosis / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015595:Skin Aging / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D013473:Sunscreening Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014466:Ultraviolet Rays; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D051152:Clusterin; D004272:DNA, Mitochondrial; D005353:Fibronectins; D012333:RNA, Messenger; D013473:Sunscreening Agents; D014316:Tritium; D001616:beta-Galactosidase; D013936:Thymidine