[The experimental study on bone marrow stem cell transplantition combinated with bushen fang therapy for DMD].
OBJECTIVE
To investigate the effect on bone marrow stem cell transplantition (BMST) combinated with Bushen fang therapy in mdx mice.


METHODS
Cultured the bone marrow cells of C56BL/6 in vitro and tranplante these cells to mdx mice after irradiation. Bushen fang was used to cure the mdx mice after BMST. The survivel rate, symptom of Graft Versus Host Disease (GVHD) and motor function of model mice following irradiation and transplantation were observed 4 or 8 months after BMST, prepared the freeze-splice of muscles of each group, and made HE staining. The dystrophin (DYS) was detected by SABC immunofluorescene.


RESULTS
Bushen fang could decrease the rate of centrally nucleated fibers (CNF), enhance expressional rate of DYS in different level and relieve the symptom of GVHD in transplanted mice.


CONCLUSIONS
Bushen fang can improve the therapeutic efficacy of BMST.
Lu
Xi-lin
XL
Department of Neurology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China.
Li
Qun
Q
Yao
Xiao-li
XL
Zhang
Wei-xi
WX
Zhang
Cheng
C
D000818:Animals; D003131:Combined Modality Therapy; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D016189:Dystrophin / Q000096:biosynthesis; D005260:Female; D006086:Graft vs Host Disease / Q000473:pathology; D018380:Hematopoietic Stem Cell Transplantation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D018482:Muscle, Skeletal / Q000378:metabolism / Q000473:pathology; D020388:Muscular Dystrophy, Duchenne / Q000188:drug therapy / Q000401:mortality / Q000628:therapy*; D008517:Phytotherapy; D010946:Plants, Medicinal / Q000737:chemistry; D015996:Survival Rate
D004365:Drugs, Chinese Herbal; D016189:Dystrophin