Biocompatibility of gutta-percha solvents using in vitro mammalian test-system.
OBJECTIVE
Taking into consideration that DNA damage and cellular death play important roles during carcinogenesis, the purpose of the present study was to evaluate in vitro genotoxic or cytotoxic effects of chloroform and eucalyptol by single cell gel (comet) assay and trypan blue exclusion test, respectively.


METHODS
Chloroform and eucalyptol were exposed to Chinese hamster ovary cells in culture directly for 3 hours at 37 degrees C at final concentrations ranging from 1.25 to 10 microL/mL. The negative control group was treated with vehicle control (phosphate-buffered solution), and the positive control group was treated with methyl metasulfonate (MMS, at 1 microg/mL concentration). All data were analyzed by the Kruskal-Wallis nonparametric test followed by the Dunn test.


RESULTS
The results showed that both gutta-percha solvents were cytotoxic at concentrations of 2.5, 5, and 10 microL/mL (P < .05). On the other hand, both solvents did not induce DNA breakage at 1.25 microL/mL concentration.


CONCLUSIONS
These results suggest that both chloroform or eucalyptol are strong cytotoxicants, but they may not be a factor that increases the level of DNA lesions in mammalian cells.
Ribeiro
Daniel A
DA
Department of Health Sciences, Federal University of São Paulo, Botucatu, Brazil. daribeiro@unifesp.br
Matsumoto
Mariza A
MA
Marques
Mariângela E A
ME
Salvadori
Daisy M F
DM
D000818:Animals; D016466:CHO Cells / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002725:Chloroform / Q000633:toxicity*; D004396:Coloring Agents; D020552:Comet Assay; D006224:Cricetinae; D003412:Cricetulus; D003511:Cyclohexanols / Q000633:toxicity*; D004249:DNA Damage; D000077591:Eucalyptol; D006171:Gutta-Percha / Q000737:chemistry; D008422:Materials Testing; D039821:Monoterpenes / Q000633:toxicity*; D019233:Retreatment; D012997:Solvents / Q000633:toxicity*; D018709:Statistics, Nonparametric; D014343:Trypan Blue
D004396:Coloring Agents; D003511:Cyclohexanols; D039821:Monoterpenes; D012997:Solvents; D002725:Chloroform; D006171:Gutta-Percha; D014343:Trypan Blue; D000077591:Eucalyptol