title: Protein profiling of microdissected pancreas carcinoma and identification of HSP27 as a potential serum marker.
abstract: BACKGROUND
  Patients with pancreatic adenocarcinomas have a poor prognosis because of late clinical manifestation and the tumor's aggressive nature. We used proteomic techniques to search for markers of pancreatic carcinoma.
  
  
  METHODS
  We performed protein profiling of microdissected cryostat sections of 9 pancreatic adenocarcinomas and 10 healthy pancreatic tissue samples using ProteinChip technology (surface-enhanced laser desorption/ionization). We identified proteins by use of 2-dimensional gel electrophoresis, peptide fingerprint mapping, and immunodepletion and used immunohistochemistry for in situ localization of the proteins found. We used ELISA to quantify these proteins in preoperative serum samples from 35 patients with pancreatic cancer and 37 healthy individuals.
  
  
  RESULTS
  From among the differentially expressed signals that were detected by ProteinChip technology, we identified 2 proteins, DJ-1 and heat shock protein 27 (HSP27). We then detected HSP27 in sera of patients by use of ELISA, indicating a sensitivity of 100% and a specificity of 84% for the recognition of pancreatic cancer.
  
  
  CONCLUSIONS
  The detection of DJ-1 and HSP27 in pure defined tissue and the retrieval of HSP27 in serum by antibody-based methods identifies a potential marker for pancreatic cancer.
authors.affiliation: Core Unit Chip Application, Institute of Human Genetics and Anthropology, Medical Faculty at Friedrich Schiller University Jena, Jena, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Melle
authors.forename: Günther
authors.initials: G
authors.lastname: Ernst
authors.forename: Niko
authors.initials: N
authors.lastname: Escher
authors.forename: Daniel
authors.initials: D
authors.lastname: Hartmann
authors.forename: Bettina
authors.initials: B
authors.lastname: Schimmel
authors.forename: Annett
authors.initials: A
authors.lastname: Bleul
authors.forename: Heike
authors.initials: H
authors.lastname: Thieme
authors.forename: Roland
authors.initials: R
authors.lastname: Kaufmann
authors.forename: Klaus
authors.initials: K
authors.lastname: Felix
authors.forename: Helmut M
authors.initials: HM
authors.lastname: Friess
authors.forename: Utz
authors.initials: U
authors.lastname: Settmacher
authors.forename: Merten
authors.initials: M
authors.lastname: Hommann
authors.forename: Konrad K
authors.initials: KK
authors.lastname: Richter
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Daffner
authors.forename: Horst
authors.initials: H
authors.lastname: Täubig
authors.forename: Thomas
authors.initials: T
authors.lastname: Manger
authors.forename: Uwe
authors.initials: U
authors.lastname: Claussen
authors.forename: Ferdinand
authors.initials: F
authors.lastname: von Eggeling
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis* / Q000473:pathology; D014408:Biomarkers, Tumor / Q000032:analysis*; D015153:Blotting, Western; D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D042282:Microdissection; D018832:Molecular Chaperones; D009363:Neoplasm Proteins / Q000032:analysis*; D010190:Pancreatic Neoplasms / Q000175:diagnosis* / Q000473:pathology; D040081:Protein Array Analysis; D020543:Proteome / Q000032:analysis*
chemical_list: D014408:Biomarkers, Tumor; D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D009363:Neoplasm Proteins; D020543:Proteome