title: Counter-current chromatography based analysis of synergy in an anti-tuberculosis ethnobotanical.
abstract: The crude extract of an Alaskan ethnobotanical plant, Oplopanax horridus, was subjected to counter-current chromatography (CCC), and the selected active regions were evaluated for their synergistic effects with an in vitro model of anti-tubercular efficacy. CCC as a support-free high-resolution separation method was employed to preclude potential irreversible absorption to a solid stationary phase. The microplate Alamar blue assay and the isobole method were used to measure the biological activity and eliminate dose-response dependent errors, respectively. Using the combination of CCC, bioassay and isobole method, significant synergistic effects were observed. Among the entire polarity range, fractions with distribution constant between 0.44 and 0.81 showed the most synergistic enhancement with an increase in potency by 108% for the recombined fractions.
authors.affiliation: Institute for Tuberculosis Research, College of Pharmacy, University of Illinois at Chicago, 833 South Wood Street, Chicago, IL 60612, USA.
authors.forename: Taichi
authors.initials: T
authors.lastname: Inui
authors.forename: Yuehong
authors.initials: Y
authors.lastname: Wang
authors.forename: Shixin
authors.initials: S
authors.lastname: Deng
authors.forename: David C
authors.initials: DC
authors.lastname: Smith
authors.forename: Scott G
authors.initials: SG
authors.lastname: Franzblau
authors.forename: Guido F
authors.initials: GF
authors.lastname: Pauli
mesh_terms: D003377:Countercurrent Distribution / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000254:growth & development; D028002:Oplopanax / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D010936:Plant Extracts