Transient beta-glucuronidase expression in lily (Lilium longflorum L.) pollen via wounding-assisted Agrobacterium-mediated transformation.
A pollen-based transient expression system has been developed. Lily pollen grains, wounded by vigorous shaking in the presence of aluminum oxide particles, were transformed by infiltration with Agrobacterium tumefaciens LBA4404 cells harboring the beta-glucuronidase (GUS) gene construct, pBI121. In histochemical and fluorometric GUS analysis, the wounding processes allowed efficient transformation and, in cDNA blot hybridization, GUS mRNA synthesis was clearly detected. Lily pollen with appropriate wounds, therefore, can be used conveniently for the rapid production of recombinant proteins.
Kim
Su-Sung
SS
Department of Biotechnology, Catholic University of Daegu, Kyungsan, Kyungbuk, Korea.
Shin
Dong-Il
DI
Park
Hee-Sung
HS
D016960:Agrobacterium tumefaciens / Q000235:genetics* / Q000254:growth & development; D015971:Gene Expression Regulation, Enzymologic; D005966:Glucuronidase / Q000235:genetics* / Q000378:metabolism; D006651:Histocytochemistry; D027762:Lilium / Q000201:enzymology / Q000235:genetics* / Q000382:microbiology; D030821:Plants, Genetically Modified; D011058:Pollen / Q000201:enzymology / Q000235:genetics* / Q000382:microbiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013314:Stress, Mechanical; D014170:Transformation, Genetic
D012333:RNA, Messenger; D005966:Glucuronidase