Evaluation of Anti- HLA Class I Antibodies in Chronic Rejection of Kidney Transplantation.
Studies have shown that patients who do not produce donor specific and /or panel reactive anti-HLA antibodies have a longer graft survival. The purpose of this study was to evaluate the posttransplant humoral immune response towards HLA-class I antigens and the measurement of the serum creatinine levels which are used in monitoring posttransplant function of kidney. Serum samples from 132 renal transplant recipients were screened for preformed anti-HLA class I panel reactive antibodies (PRA) by means of microlymphocytotoxicity assay. The results revealed the presence of PRA in 26 (19.7%) out of 132 transplanted patients. Graft function was evaluated by measurement of serum creatinine levels which revealed the mean of 1.75 mg/dl (SD: 1.08). Because of clinical significance of presence of different PRA amounts (>10%, > 20% and >50% of panel reactivity) in patients, correlation with kidney function status was analyzed. The obtained data highlighted a higher presence of serum creatinine levels in PRA-positive patients compared to negative patients (P<0.01). These results (and further studies for class II, ...) can be used to implement new therapeutic strategies to curtail post transplant alloantibodies production and better allografts survival.
Nicknam
Mohammad Hossein
MH
Immunology Department, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran. nicknam_m@yahoo.com.
Torkashvand
Ali
A
Ghods
Ahmad
A
Amirzargar
Ali Akbar
AA
Amirkhani
Aref
A
Khosravi
Farideh
F
Nikbin
Behrouz
B