title: Spectrophotometric and spectrofluorimetric methods for the determination of tranexamic acid in pharmaceutical formulation.
abstract: Two simple and sensitive spectrophotometric and fluorimetric methods for the determination of tranexamic acid in tablets are developed. The methods are based on condensation the primary amino group of tranexamic acid with acetyl acetone and formaldehyde producing a yellow coloured product, which is measured spectrophotometrically at 335 nm or fluorimetrically at 480 nm the colour was stable for at least 1 h. Beer's law was valid within a concentration rang of 0.05-2.0 microg ml-1 spectrophotometrically and 0.05-0.25 microg ml-1 fluorimetrically. All the variables were studied to optimize the reaction conditions. No interference was observed in the presence of common pharmaceutical excipints. The validity of both methods was tested by analyzing tranexamic acid in its pharmaceutical preparations. Good recoveries were obtained and the results were comparable with those obtained by standard method.
authors.affiliation: Pharmaceutical Chemistry Department, Faculty of Pharmacy, Al-Fateh University, Tripoli, Libya.
authors.forename: Khalifa Abulghasem
authors.initials: KA
authors.lastname: El-Aroud
authors.forename: Adel Mohamed
authors.initials: AM
authors.lastname: Abushoffa
authors.forename: Hisham Ezzat
authors.initials: HE
authors.lastname: Abdellatef
mesh_terms: D002626:Chemistry, Pharmaceutical; D015394:Molecular Structure; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013053:Spectrophotometry / Q000379:methods*; D014148:Tranexamic Acid / Q000737:chemistry*
chemical_list: D014148:Tranexamic Acid