title: ESTs from a wild Arachis species for gene discovery and marker development.
abstract: BACKGROUND
  Due to its origin, peanut has a very narrow genetic background. Wild relatives can be a source of genetic variability for cultivated peanut. In this study, the transcriptome of the wild species Arachis stenosperma accession V10309 was analyzed.
  
  
  RESULTS
  ESTs were produced from four cDNA libraries of RNAs extracted from leaves and roots of A. stenosperma. Randomly selected cDNA clones were sequenced to generate 8,785 ESTs, of which 6,264 (71.3%) had high quality, with 3,500 clusters: 963 contigs and 2537 singlets. Only 55.9% matched homologous sequences of known genes. ESTs were classified into 23 different categories according to putative protein functions. Numerous sequences related to disease resistance, drought tolerance and human health were identified. Two hundred and six microsatellites were found and markers have been developed for 188 of these. The microsatellite profile was analyzed and compared to other transcribed and genomic sequence data.
  
  
  CONCLUSIONS
  This is, to date, the first report on the analysis of transcriptome of a wild relative of peanut. The ESTs produced in this study are a valuable resource for gene discovery, the characterization of new wild alleles, and for marker development. The ESTs were released in the [GenBank:EH041934 to EH048197].
authors.affiliation: Departamento de Biologia Celular, Universidade de Brasília, Campus I, Brasília, DF. Brazil. proite@cenargen.embrapa.br
authors.forename: Karina
authors.initials: K
authors.lastname: Proite
authors.forename: Soraya C M
authors.initials: SC
authors.lastname: Leal-Bertioli
authors.forename: David J
authors.initials: DJ
authors.lastname: Bertioli
authors.forename: Márcio C
authors.initials: MC
authors.lastname: Moretzsohn
authors.forename: Felipe R
authors.initials: FR
authors.lastname: da Silva
authors.forename: Natalia F
authors.initials: NF
authors.lastname: Martins
authors.forename: Patrícia M
authors.initials: PM
authors.lastname: Guimarães
mesh_terms: D010367:Arachis / Q000235:genetics*; D020224:Expressed Sequence Tags; D015723:Gene Library; D017343:Genes, Plant; D005819:Genetic Markers; D018745:Genome, Plant; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein
chemical_list: D005819:Genetic Markers