Oligonucleotide microarrays for the study of coastal microbial communities.
DNA microarrays are well suited as a tool for analyzing functional gene diversity as well as community composition in aquatic environments. Microarrays allow for the semi-quantitative characterization of target genes by means of specific hybridization of labeled target gene sequences, amplified from the environment, to the corresponding oligonucleotide probes on the slide. Specificity and sensitivity are determined by the probe design. In their current implementation, environmental DNA microarrays are useful for analyzing microbial communities as well as for analyzing the presence of functional genes involved in larger biogeochemical processes, such as nitrogen cycling. Here, we lay out a basic protocol to analyze genes in the environment, which can be applied to most target genes of interest.
Taroncher-Oldenburg
Gaspar
G
Nature Biotechnology, NPG America Inc., New York, NY, USA.
Ward
Bess B
BB
D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D017753:Ecosystem; D019015:Geologic Sediments / Q000382:microbiology; D015336:Molecular Probe Techniques; D015335:Molecular Probes; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods* / Q000706:statistics & numerical data; D016415:Sequence Alignment; D013816:Thermodynamics; D014871:Water Microbiology
D004269:DNA, Bacterial; D015335:Molecular Probes