[The effect of transforming growth factor-beta1 on EDA region of fibronectin in oral squamous cell carcinoma and adenoid cystic carcinoma cells].
OBJECTIVE
To investigate the effect of transforming growth factor-beta1 (TGF-beta1) on EDA region of fibronectin in oral squamous cell carcinoma and adenoid cystic carcinoma cells.


METHODS
Immunohistochemistry and reverse transcription polymerase chain reaction technique were used to detect the changes of EDA proteins and mRNAs expression.


RESULTS
The immunostaining ratio in Tca83 cells with TGF-beta1 was greatly higher than that in Tca83 cells without TGF-beta1 (P < 0.01), the immunostaining ratio in SACC-83 cells with TGF-beta1 was higher than that in SACC-83 cells without TGF-beta1 (P < 0.05), but there was no significant difference between SACC-LM cells with TGF-beta1 and SACC-LM cells without TGF-beta1 (P > 0.05). In the three oral carcinoma cells, the expression of EDA(+) mRNAs in the group with TGF-beta1 was higher than that in the group without TGF-beta1 (P < 0.05). The expression of EDA(-) mRNAs in the group with TGF-beta1 was lower than that in the group without TGF-beta1 (P < 0.05).


CONCLUSIONS
TGF-beta1 could influence EDA region splicings and upregulate the expression of EDA region in Tca83, SACC-83 and SACC-LM cells, and might play an important role in accelerating oral carcinoma cells adhesion and tumor invasion and metastasis.
Zhang
Jian-peng
JP
Department of Cariology and Endodontology, Capital Medical University, School of Stomatology, Beijing 100050, China.
Li
Cui-ying
CY
D003528:Carcinoma, Adenoid Cystic / Q000378:metabolism / Q000473:pathology*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D009062:Mouth Neoplasms / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*
D005353:Fibronectins; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1