Characterization of the gene expression related to the process of DNA damage tolerance in Schistosoma mansoni.
In the course of its complex life cycle, the parasite Schistosoma mansoni need to adapt to distinct environments, and consequently is exposed to various DNA damaging agents. The Schistosoma genome sequencing initiative has uncovered sequences from genes and transcripts related to the process of DNA damage tolerance as the enzymes UBC13, MMS2, and RAD6. In the present work, we evaluate the importance of this process in different stages of the life cycle of this parasite. The importance is evidenced by expression and phylogenetic profiles, which show the conservation of this pathway from protozoa to mammalians on evolution.
Silva
S H
SH
Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, 14049-900 Ribeirão Preto, SP, Brazil.
Pereira-Júnior
O S
OS
Silva
C S
CS
Brigatto
O M
OM
Macedo
E
E
Rodrigues
V
V
D000818:Animals; D004249:DNA Damage; D018509:DNA, Helminth / Q000235:genetics*; D020869:Gene Expression Profiling; D008018:Life Cycle Stages; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012550:Schistosoma mansoni / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D044763:Ubiquitin-Conjugating Enzymes / Q000235:genetics*
D018509:DNA, Helminth; D044763:Ubiquitin-Conjugating Enzymes