Local implantation of autologous mononuclear cells from bone marrow and peripheral blood for treatment of ischaemic digits in patients with connective tissue diseases.
OBJECTIVE
CD34-positive bone marrow mononuclear cells (MNCs) have been successfully used for regeneration of small arteries in Buerger's disease. The objective of this study is to examine the angiogenetic potential of autologous MNCs from bone marrow and peripheral blood implanted into the ischaemic digits from patients with connective tissue diseases.


METHODS
Three patients with systemic sclerosis, two with mixed connective tissue disease, and one with CREST syndrome were enrolled who had painful ischaemic digits with necrosis refractory to several vasodilators including intravenous prostaglandins. MNCs obtained from 7 ml/kg bone marrow blood and 400 ml peripheral blood were implanted into 20 different sites in palms and/or soles. The study was performed open-labelled.


RESULTS
Pain in the numeric rating scale improved remarkably up to 1 month after implantation of bone marrow or peripheral MNCs to the same extent, although no significant differences were found in transcutaneous oxygen pressure and thermogram before and after the implantation. Bone marrow MNCs increased blood flow of the hand determined by intra-arterial digital subtraction angiography, while peripheral MNCs did not.


CONCLUSIONS
Implantation of autologous MNCs from peripheral and bone marrow into the ischaemic digits was so effective in pain-relief and more clinical trials would be warranted to see whether this could be a new treatment modality for angiogenesis in connective tissue diseases as in Buerger's disease.
Kamata
Y
Y
Division of Rheumatology and Clinical Immunology, Jichi Medical University, Shimotsuke-shi, Tochigi-ken, Japan. y.kamata@jichi.ac.jp
Takahashi
Y
Y
Iwamoto
M
M
Matsui
K
K
Murakami
Y
Y
Muroi
K
K
Ikeda
U
U
Shimada
K
K
Yoshio
T
T
Okazaki
H
H
Minota
S
S
D000368:Aged; D015901:Angiography, Digital Subtraction; D018952:Antigens, CD34 / Q000032:analysis; D016026:Bone Marrow Transplantation; D017675:CREST Syndrome / Q000628:therapy; D003240:Connective Tissue Diseases / Q000150:complications / Q000628:therapy*; D005260:Female; D005385:Fingers / Q000098:blood supply; D006801:Humans; D007511:Ischemia / Q000000981:diagnostic imaging / Q000209:etiology / Q000628:therapy*; D008297:Male; D008875:Middle Aged; D018919:Neovascularization, Physiologic; D036102:Peripheral Blood Stem Cell Transplantation; D014034:Toes / Q000098:blood supply; D016896:Treatment Outcome
D018952:Antigens, CD34