Osteopontin expression and function within the dorsal root ganglion.
Osteopontin expression has previously been demonstrated in the adult rat dorsal root ganglion, although its function remains unclear. Here, we demonstrate, using real-time reverse transcription-polymerase (RT-PCR) chain reaction, that osteopontin mRNA expression is increased 1 and 3 weeks following sciatic nerve section (axotomy). Further, immunohistochemical staining suggests that this increase is restricted to neurons already expressing the protein. Osteopontin knock-out animals have significantly increased mechanosensory thresholds in the intact adult compared with the wild-type controls; however no differences in allodynia are noted between genotypes using a model of neuropathic pain. Lastly, exogenous recombinant osteopontin has no effect on neurite outgrowth from adult wild-type sensory neurons, nor were differences in neurite outgrowth observed in osteopontin knock-out animals compared with wild-type controls.
Marsh
Barnaby C L
BC
Department of Pharmacology at South Bristol, University of Bristol, Bristol, UK.
Kerr
Niall C
NC
Isles
Nathalie
N
Denhardt
David T
DT
Wynick
David
D
D000367:Age Factors; D000818:Animals; D019771:Axotomy; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology / Q000502:physiology*; D015870:Gene Expression / Q000502:physiology; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D009437:Neuralgia / Q000378:metabolism / Q000503:physiopathology*; D016501:Neurites / Q000502:physiology; D009475:Neurons, Afferent / Q000502:physiology / Q000648:ultrastructure; D009619:Nociceptors / Q000502:physiology; D053495:Osteopontin / Q000235:genetics* / Q000378:metabolism*; D017288:Pain Threshold / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012584:Sciatic Nerve / Q000502:physiology
D012333:RNA, Messenger; D053495:Osteopontin