Evaluation of Etest and disk diffusion methods compared with broth microdilution antifungal susceptibility testing of clinical isolates of Candida spp. against posaconazole.
We performed Etest, disk diffusion, and broth microdilution susceptibility testing of 2,171 clinical isolates of Candida spp. against posaconazole. By using provisional breakpoints for comparison purposes only, the categorical agreement between the agar-based methods and broth microdilution results ranged from 93 to 98%, with <1% very major errors. The essential agreement (within 2 well dilutions) between the Etest and broth microdilution methods was 94%. These agar-based methods hold promise as simple and reliable methods for determination of the posaconzole susceptibilities of Candida spp.
Diekema
Daniel J
DJ
Department of Pathology, Roy J. and Lucille A. Carver College of Medicine and College of Public Health, University of Iowa, Iowa City, IA 52242, USA. daniel-diekema@uiowa.edu
Messer
Shawn A
SA
Hollis
Richard J
RJ
Boyken
Linda B
LB
Tendolkar
Shailesh
S
Kroeger
Jennifer
J
Pfaller
Michael A
MA
D000362:Agar; D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000145:classification* / Q000187:drug effects* / Q000302:isolation & purification; D002177:Candidiasis / Q000382:microbiology; D003470:Culture Media; D052978:Disk Diffusion Antimicrobial Tests / Q000379:methods* / Q000592:standards; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods* / Q000592:standards; D014230:Triazoles / Q000494:pharmacology*
D000935:Antifungal Agents; D003470:Culture Media; D014230:Triazoles; C101425:posaconazole; D000362:Agar