title: A panel of monoclonal antibodies recognizing the Staphylococcus epidermidis fibrinogen-binding MSCRAMM SdrG.
abstract: Staphylococcus epidermidis is an important opportunistic human pathogen that has recently emerged as a major cause of foreign-body infections. The most important stage contributing to the pathogenesis of this bacteria is the initial adherence to host tissue. SdrG is a cell-wall-anchored fibrinogen-binding adhesin of S. epidermidis that has been shown to be necessary for bacterial binding to fibrinogen-coated foreign bodies, such as catheters. Here we report the generation and characterization of a panel of monoclonal antibodies (MAbs) directed against this S. epidermidis virulence factor. Through the use of multiple in vitro assays, surface plasmon resonance, and flow cytometry, we have characterized a diverse array of MAbs that may prove to be beneficial in studies that address the precise biologic role of SdrG.
authors.affiliation: Inhibitex, Inc., Alpharetta, Georgia 30004, USA.
authors.forename: Andrea E
authors.initials: AE
authors.lastname: Hall
authors.forename: Pratiksha R
authors.initials: PR
authors.lastname: Patel
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Domanski
authors.forename: Bradley D
authors.initials: BD
authors.lastname: Prater
authors.forename: Elena L
authors.initials: EL
authors.lastname: Gorovits
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Syribeys
authors.forename: John H
authors.initials: JH
authors.lastname: Vernachio
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Patti
authors.forename: Jeff T
authors.initials: JT
authors.lastname: Hutchins
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005340:Fibrinogen / Q000378:metabolism*; D013294:Lactococcus lactis / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011485:Protein Binding / Q000276:immunology; D013212:Staphylococcus epidermidis / Q000235:genetics / Q000276:immunology*
chemical_list: D018829:Adhesins, Bacterial; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D002352:Carrier Proteins; C112556:Fbe protein, bacteria; C509820:MSCRAMM proteins, Staphylococcus; D005340:Fibrinogen