title: Re-expression of proteins involved in cytokinesis during cardiac hypertrophy.
abstract: Cardiomyocytes stop dividing after birth and postnatal heart growth is only achieved by increase in cell volume. In some species, cardiomyocytes undergo an additional incomplete mitosis in the first postnatal week, where karyokinesis takes place in the absence of cytokinesis, leading to binucleation. Proteins that regulate the formation of the actomyosin ring are known to be important for cytokinesis. Here we demonstrate for the first time that small GTPases like RhoA along with their downstream effectors like ROCK I, ROCK II and Citron Kinase show a developmental stage specific expression in heart, with high levels being expressed in cardiomyocytes only at stages when cytokinesis still occurs (i.e. embryonic and perinatal). This suggests that downregulation of many regulatory and cytoskeletal components involved in the formation of the actomyosin ring may be responsible for the uncoupling of cytokinesis from karyokinesis in rodent cardiomyocytes after birth. Interestingly, when the myocardium tries to adapt to the increased workload during pathological hypertrophy a re-expression of proteins involved in DNA synthesis and cytokinesis can be detected. Nevertheless, the adult cardiomyocytes do not appear to divide despite this upregulation of the cytokinetic machinery. The inability to undergo complete division could be due to the presence of stable, highly ordered and functional sarcomeres in the adult myocardium or could be because of the inefficiency of degradation pathways, which facilitate the division of differentiated embryonic cardiomyocytes by disintegrating myofibrils.
authors.affiliation: Institute of Cell Biology, ETH Zürich-Hönggerberg, CH-8093 Zürich, Switzerland.
authors.forename: Preeti
authors.initials: P
authors.lastname: Ahuja
authors.forename: Evelyne
authors.initials: E
authors.lastname: Perriard
authors.forename: Thierry
authors.initials: T
authors.lastname: Pedrazzini
authors.forename: Shinji
authors.initials: S
authors.lastname: Satoh
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Perriard
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Ehler
mesh_terms: D000205:Actomyosin / Q000378:metabolism; D000577:Amides / Q000494:pharmacology; D000804:Angiotensin II / Q000378:metabolism; D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology; D015415:Biomarkers / Q000032:analysis; D002154:Calpain / Q000378:metabolism; D006332:Cardiomegaly / Q000139:chemically induced / Q000378:metabolism*; D048750:Cell Nucleus Division; D044842:Cullin Proteins / Q000378:metabolism; D048749:Cytokinesis; D006321:Heart / Q000196:embryology* / Q000254:growth & development; D006973:Hypertension / Q000473:pathology; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D051379:Mice; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000502:physiology*; D009210:Myofibrils / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011725:Pyridines / Q000494:pharmacology; D051381:Rats; D020303:Rats, Inbred Dahl; D015854:Up-Regulation; D020741:rho GTP-Binding Proteins / Q000378:metabolism; D054460:rho-Associated Kinases
chemical_list: D000577:Amides; D000959:Antihypertensive Agents; D015415:Biomarkers; D044842:Cullin Proteins; D047908:Intracellular Signaling Peptides and Proteins; D011725:Pyridines; D000804:Angiotensin II; C108830:Y 27632; D000205:Actomyosin; D017346:Protein Serine-Threonine Kinases; D054460:rho-Associated Kinases; D002154:Calpain; D020741:rho GTP-Binding Proteins