Supplementation of enrichment broths by novobiocin for detecting Shiga toxin-producing Escherichia coli from food: a controversial use.
OBJECTIVE
To investigate the assumption that usage of novobiocin (20 mg l(-1)) in Shiga toxin-producing Escherichia coli (STEC) enrichment broths could achieve false-negative results.


RESULTS
First, the minimum inhibitory concentration (MIC) of 74 E. coli O157:H7 and 55 non-O157:H7 STEC strains to novobiocin was determined. Second, to visualize the potential impact of novobiocin on the STEC growth during the enrichment step, the growth experiments were carried out in trypticase soy broth (TSB) with and without 20 mg l(-1) of novobiocin. The MIC values varied from 32 to > 64 mg l(-1) for the 74 E. coli O157:H7 strains, and from 16 to > 64 mg l(-1) for the 55 non-O157:H7 STEC strains. The E. coli O157:H7 strains were significantly (P < 0.001) more resistant to novobiocin than the non-O157:H7 STEC strains. The present study shows that the addition of novobiocin into enrichment broths inhibits the growth of some non-O157:H7 STEC strains, and slows down the growth of some STEC strains.


CONCLUSIONS
Enrichment broths supplemented by novobiocin could lead to false-negative results for detecting STEC from food.


CONCLUSIONS
We strongly suggest that novobiocin should not be systematically added into enrichment broths for detecting STEC from food.
Vimont
A
A
Unité de Microbiologie Alimentaire et Prévisionnelle, Ecole Nationale Vétérinaire de Lyon, France. a.vimont@vet-lyon.fr
Delignette-Muller
M-L
ML
Vernozy-Rozand
C
C
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003470:Culture Media; D004926:Escherichia coli / Q000187:drug effects / Q000302:isolation & purification* / Q000378:metabolism; D019453:Escherichia coli O157 / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism; D005516:Food Microbiology; D008826:Microbial Sensitivity Tests; D009675:Novobiocin / Q000494:pharmacology*; D022603:Shiga Toxins / Q000378:metabolism
D000900:Anti-Bacterial Agents; D003470:Culture Media; D022603:Shiga Toxins; D009675:Novobiocin