Osteogenic differentiation of rabbit mesenchymal stem cells in thermo-reversible hydrogel constructs containing hydroxyapatite and bone morphogenic protein-2 (BMP-2).
The aim of this study was to assess the efficacy of ectopic bone formation in a three-dimensional hybrid scaffold in combination with hydroxyapatite (HA) and poly(NiPAAm-co-AAc) as an injectable vehicle in the form of a supporting matrix for the osteogenic differentiation of rabbit mesenchymal stem cells (MSCs). Osteogenic differentiation of MSCs in the hybrid scaffold was greatly influenced by the addition of growth factors. When the osteoinduction activity of hybrid scaffold was studied following implantation into the back subcutis of nude mouse in terms of histological and biochemical examinations, significantly homogeneous bone formation was histologically observed throughout the hybrid scaffolds containing growth factor (BMP-2: bone morphogenic protein-2). The level of alkaline phosphatase activity and osteocalcin content at the implanted sites of hybrid scaffolds were significantly high for the perfusion group compared with those in static culture group. We conclude that combination of MSC-seeded hybrid scaffold containing BMP-2 was a promising method by which to enhance in vitro osteogenic differentiation of MSC and in vivo ectopic bone formation.
Na
Kun
K
Division of Biotechnology, The Catholic University of Korea, 43-1 Yokkok2-dong, Wonmi-gu, Bucheon 420-743, Republic of Korea.
Kim
Sung Won
SW
Sun
Bo Kyung
BK
Woo
Dae Gyun
DG
Yang
Han Na
HN
Chung
Hyung Min
HM
Park
Keun Hong
KH
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000737:chemistry / Q000378:metabolism*; D002113:Calcification, Physiologic; D002232:Carbocyanines / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism; D020100:Hydrogels / Q000737:chemistry / Q000378:metabolism; D006882:Hydroxyapatites / Q000737:chemistry / Q000378:metabolism*; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D051379:Mice; D008819:Mice, Nude; D010012:Osteogenesis / Q000502:physiology*; D011817:Rabbits; D013696:Temperature; D016212:Transforming Growth Factor beta / Q000737:chemistry / Q000378:metabolism*
D001672:Biocompatible Materials; C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; C098793:CY5.5 cyanine dye; D002232:Carbocyanines; D024042:Collagen Type I; D024043:Collagen Type II; D020100:Hydrogels; D006882:Hydroxyapatites; D016212:Transforming Growth Factor beta; D000469:Alkaline Phosphatase