Direct determination of non-steroidal anti-inflammatory drugs by column-switching LC-MS.
A method for determination of 16 non-steroidal anti-inflammatory drugs (NSAIDs) in human plasma samples without time-consuming sample pre-treatments was developed. The system consisted of two pumps for mobile phase delivery, a six-port switching valve, a pre-column (Oasis HLB Cartridge Column), and a reversed phase analytical column (COSMOSIL 3C18-MS-II). The analytes were trapped on the precolumn and subsequently separated on the analytical column. The present method allowed on-line sample clean-up and enrichment, leading to improved sensitivity without any tedious sample preparation. The recoveries of NSAIDs from human plasma by column-switching were greater than 72.6%. The total analysis time for a single analytical run was approximately 11 min. The detection limits of NSAIDs were 0.0025 to 0.2 microg/mL using the selected ion monitoring mode.
Suenami
Koichi
K
Department of Chemistry, Faculty of Engineering, Gifu University, Gifu, Japan. c18871@pref.gifu.lg.jp
Wah Lim
Lee
L
Takeuchi
Toyohide
T
Sasajima
Yasuhide
Y
Sato
Kiyohito
K
Takekoshi
Yuji
Y
Kanno
Susumu
S
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis*; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*
D000894:Anti-Inflammatory Agents, Non-Steroidal