Characterisation and bioactivity of protein-bound polysaccharides from submerged-culture fermentation of Coriolus versicolor Wr-74 and ATCC-20545 strains.
The protein-bound polysaccharides of Coriolus versicolor (CPS) have been reported to stimulate overall immune functions against cancers and various infectious diseases by activating specific cell functions. A New Zealand isolate (Wr-74) and a patented strain (ATCC-20545) of C. versicolor were compared in this study. The fruit bodies of both strains were grown for visual verification. Both strains were grown in submerged-culture using an airlift fermentor with milk permeate as the base medium supplemented with glucose, yeast extract and salt. Metabolic profiles of both strains obtained over 7-day fermentation showed very similar trends in terms of biomass production (8.9-10.6 mg/ml), amounts of extracellular polysaccharide (EPS) from the culture medium (1150-1132 microg/ml), and intracellular polysaccharide (IPS) from the mycelium (80-100 microg/ml). Glucose was the dominant sugar in both EPS and IPS, and the polymers each consisted of three molecular weight fractions ranging from 2 x 10(6) to 3 x 10(3 )Da. Both the EPS and IPS were able to significantly induce cytokine production (interleukin 12 and gamma interferon) in murine splenocytes in vitro. Highest levels of interleukin 12 (291 pg/ml) and gamma interferon (6,159 pg/ml) were obtained from samples containing Wr-74 IPS (0.06 microg/ml) and ATCC 20545 IPS (0.1 microg/ml), respectively. The results indicated that lower levels of EPS and IPS generally resulted in higher immune responses than did higher polymer concentrations.
Cui
Jian
J
Riddet Centre, Massey University, Private Bag 11 222, Palmerston North, New Zealand.
Goh
Kelvin Kim Tha
KK
Archer
Richard
R
Singh
Harjinder
H
D000596:Amino Acids / Q000737:chemistry; D018533:Biomass; D003470:Culture Media; D016207:Cytokines / Q000096:biosynthesis; D005285:Fermentation; D048690:Fruiting Bodies, Fungal / Q000254:growth & development; D005656:Fungal Proteins / Q000737:chemistry; D008828:Microbiological Techniques / Q000295:instrumentation; D008970:Molecular Weight; D020072:Polyporales / Q000145:classification / Q000254:growth & development / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*
D000596:Amino Acids; D003470:Culture Media; D016207:Cytokines; D005656:Fungal Proteins; D011134:Polysaccharides