title: Description of a new species, Bifidobacterium crudilactis sp. nov., isolated from raw milk and raw milk cheeses.
abstract: A new Bifidobacterium species is described based on the study of ten Gram-positive strains with fructose-6-phosphate phosphoketolase activity. They are part of a phenotypic group comprising 141 strains isolated from raw milk and raw milk cheeses in French raw milk cheese factories. This group was separated by a numerical analysis based on API 50CH, API 32A tests and growth at 46 degrees C. A strong similarity of 16S rRNA sequences (99.8%) was shown between strain FR62/b/3(T) and Bifidobacterium psychraerophilum LMG 21775(T). However, low DNA-DNA relatedness was observed between their DNAs (31%). The new isolates are able to grow at low temperatures (all ten strains up to 5 degrees C) and strain FR62/b/3(T) grows under aerobic conditions, as does B. psychraerophilum. However, contrary to B. psychraerophilum, they do not ferment L-arabinose, D-xylose, arbutin or melezitose, but they do acidify lactose. The DNA G+C content of FR62/b/3(T) is 56.4mol%. Therefore, the name Bifidobacterium crudilactis sp. nov. is proposed, with its type strain being FR62/b/3(T) (=LMG 23609(T)=CNCM I-3342(T)).
authors.affiliation: Faculty of Veterinary Medicine, Food Sciences Department, University of Liege, Sart Tilman, B43b, B-4000 Liege, Belgium.
authors.forename: V
authors.initials: V
authors.lastname: Delcenserie
authors.forename: F
authors.initials: F
authors.lastname: Gavini
authors.forename: H
authors.initials: H
authors.lastname: Beerens
authors.forename: O
authors.initials: O
authors.lastname: Tresse
authors.forename: C
authors.initials: C
authors.lastname: Franssen
authors.forename: G
authors.initials: G
authors.lastname: Daube
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001644:Bifidobacterium / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D002611:Cheese / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis*; D004275:DNA, Ribosomal / Q000032:analysis; D008892:Milk / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000032:analysis* / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S