Improved synthesis methods of standards used for quantitative determination of total isothiocyanates from broccoli in human urine.
A well-known method for quantification of isothiocyanates (ITCs) and their metabolites is the condensation reaction with 1,2-benzenedithiole to produce 1,3-benzodithiole-2-thione, which can be quantified by high-performance liquid chromatography. Standards of an ITC metabolite and 1,3-benzodithiole-2-thione are required for this assay but are not commercially available. In the present study, we report on an improved synthesis of the ITC metabolite N-acetyl-S-(N-4-methylsulfinylbutylthiocarbamoyl)-L-cysteine and 1,3-benzodithiole-2-thione. The standards were used to quantify the urinary excretion of ITCs from 10 healthy subjects who consumed 350 g broccoli. The excretion was investigated throughout 48 h showing a cumulative urinary ITC excretion of 49.1+/-25.2% of the dose.
Kristensen
Mette
M
Department of Toxicology and Risk Assessment, Danish Institute for Food and Veterinary Research, 2860 Søborg, Denmark. mehxy@dfvf.dk
Krogholm
Kirstine S
KS
Frederiksen
Hanne
H
Duus
Fritz
F
Cornett
Claus
C
Bügel
Susanne H
SH
Rasmussen
Salka E
SE
D000328:Adult; D001937:Brassica / Q000737:chemistry*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D006801:Humans; D017879:Isothiocyanates / Q000592:standards / Q000652:urine*; D008297:Male; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet
D017879:Isothiocyanates