Novel multi-gas chromatography-olfactometry device and software for the identification of odour-active compounds.
The aim of this work was to devise a novel fully computerised chromatography-olfactometry system where eight sniffers detect volatile compounds obtained from a single chromatographic separation. The technical options taken to implement this system are described in detail. The new methods and software developed and applied to analyse data obtained by gas chromatography-olfactometry and mass spectrometry (GC-O/MS) are presented. We found that 46 odorous zones could be detected by a panel of eight sniffers during the 35min of the analysis of the volatile compounds extracted from a cheese by purge-and-trap. Synchronisation of olfactometry and mass spectrometry data enabled us to propose 44 structures to account for 44 out of these 46 odorous zones.
Berdagué
J L
JL
INRA, UR370 QuaPA, Equipe Typicité Aromatique et Authentification, F-63122 Saint Genès Champanelle, France. berdague@clermont.inra.fr
Tournayre
P
P
Cambou
S
S
D002611:Cheese / Q000032:analysis; D005504:Food Analysis / Q000379:methods*; D005638:Fruit / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D009812:Odorants / Q000032:analysis*; D015203:Reproducibility of Results; D012984:Software; D014835:Volatilization; D015014:Yogurt / Q000032:analysis