title: Involvement of Na+/Ca2+ exchanger type-1 in ischemia-induced neovascularization in the mouse hindlimb.
abstract: The Na+/Ca2+ exchanger (NCX) is considered to be involved in endothelial nitric oxide (NO) production and endothelium-dependent vasorelaxation, but little is known about the physiological and pathological roles of endothelial NCX in these processes. We examined the role of NCX1 in neovascularization in mice with hindlimb ischemia. Unilateral hindlimb ischemia was induced surgically in wild-type and heterozygous NCX1 knockout mice (NCX1+/-) mice. We found that in NCX1+/- mice, blood flow recovery was significantly augmented compared with that in wild-type mice. N(G)-nitro-L-arginine methyl ester treatment eliminated enhanced angiogenesis observed in NCX1+/- mice. These results suggest that NCX1 is involved in eNOS-dependent angiogenesis.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Fukuoka University, Fukuoka 814-0180, Japan.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Matsui
authors.forename: Satomi
authors.initials: S
authors.lastname: Kita
authors.forename: Takeshi
authors.initials: T
authors.lastname: Katsuragi
authors.forename: Issei
authors.initials: I
authors.lastname: Komuro
authors.forename: Takahiro
authors.initials: T
authors.lastname: Iwamoto
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ohjimi
mesh_terms: D000818:Animals; D006614:Hindlimb / Q000098:blood supply*; D007511:Ischemia / Q000503:physiopathology*; D051379:Mice; D018345:Mice, Knockout; D009389:Neovascularization, Pathologic; D019831:Sodium-Calcium Exchanger / Q000235:genetics / Q000502:physiology*
chemical_list: D019831:Sodium-Calcium Exchanger; C110374:sodium-calcium exchanger 1