title: Near full-length genome characterization of three additional HIV type 1 CRF13_cpx strains from Cameroon.
abstract: Recombinant forms of HIV-1 contribute significantly to the ongoing epidemic. In the present study, we characterized the near full-length genomes of three candidate HIV-1 CRF13_cpx strains originating in Cameroon, 04CM-173-9, 04CM-632-28, and 02CM-A1394. Bootscanning, recombination breakpoint analysis, and phylogenetic trees confirmed similar genomic structures with identical breakpoint positions compared to the three available CRF13_cpx sequences. The candidate and reference sequences formed a distinct cluster well separated from other group M subtypes and had a mosaic structure derived from subtypes A1, G, J, and CRF01_AE. The similarity in genomic composition and position of recombination breakpoints suggest that these isolates share a common ancestor. The epidemiological significance of CRF13_cpx strains in Cameroon is unknown; however, the availability of three additional genomic sequences will improve our understanding of the overall genetic diversity within this recombinant form of HIV-1.
authors.affiliation: Abbott Diagnostics, AIDS Research and Retrovirus Discovery, Abbott Laboratories, 100 Abbott Park Road, Abbott Park, IL 60064, USA.
authors.forename: Ka-Cheung
authors.initials: KC
authors.lastname: Luk
authors.forename: Vera
authors.initials: V
authors.lastname: Holzmayer
authors.forename: Julie
authors.initials: J
authors.lastname: Yamaguchi
authors.forename: Priscilla
authors.initials: P
authors.lastname: Swanson
authors.forename: Catherine A
authors.initials: CA
authors.lastname: Brennan
authors.forename: Charlotte
authors.initials: C
authors.lastname: Ngansop
authors.forename: Dora
authors.initials: D
authors.lastname: Mbanya
authors.forename: Hermine
authors.initials: H
authors.lastname: Gayum
authors.forename: Marcelline Ngounoue
authors.initials: MN
authors.lastname: Djuidje
authors.forename: Nicaise
authors.initials: N
authors.lastname: Ndembi
authors.forename: Donatien
authors.initials: D
authors.lastname: Kamdem
authors.forename: Lazare
authors.initials: L
authors.lastname: Kaptué
authors.forename: Lutz
authors.initials: L
authors.lastname: Gürtler
authors.forename: Sushil G
authors.initials: SG
authors.lastname: Devare
authors.forename: John
authors.initials: J
authors.lastname: Hackett
mesh_terms: D000328:Adult; D002163:Cameroon; D005260:Female; D015658:HIV Infections / Q000235:genetics*; D015497:HIV-1 / Q000145:classification / Q000235:genetics*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D016865:Reassortant Viruses / Q000145:classification / Q000235:genetics*; D017423:Sequence Analysis, RNA