The Helicobacter pylori amidotransferase GatCAB is equally efficient in glutamine-dependent transamidation of Asp-tRNAAsn and Glu-tRNAGln.
The amide aminoacyl-tRNAs, Gln-tRNA(Gln) and Asn-tRNA(Asn), are formed in many bacteria by a pretranslational tRNA-dependent amidation of the mischarged tRNA species, Glu-tRNA(Gln) or Asp-tRNA(Asn). This conversion is catalyzed by a heterotrimeric amidotransferase GatCAB in the presence of ATP and an amide donor (Gln or Asn). Helicobacter pylori has a single GatCAB enzyme required in vivo for both Gln-tRNA(Gln) and Asn-tRNA(Asn) synthesis. In vitro characterization reveals that the enzyme transamidates Asp-tRNA(Asn) and Glu-tRNA(Gln) with similar efficiency (k(cat)/K(m) of 1368.4 s(-1)/mM and 3059.3 s(-1)/mM respectively). The essential glutaminase activity of the enzyme is a property of the A-subunit, which displays the characteristic amidase signature sequence. Mutations of the GatA catalytic triad residues (Lys(52), Ser(128), Ser(152)) abolished glutaminase activity and consequently the amidotransferase activity with glutamine as the amide donor. However, the latter activity was rescued when the mutant enzymes were presented with ammonium chloride. The presence of Asp-tRNA(Asn) and ATP enhances the glutaminase activity about 22-fold. H. pylori GatCAB uses the amide donor glutamine 129-fold more efficiently than asparagine, suggesting that GatCAB is a glutamine-dependent amidotransferase much like the unrelated asparagine synthetase B. Genomic analysis suggests that most bacteria synthesize asparagine in a glutamine-dependent manner, either by a tRNA-dependent or in a tRNA-independent route. However, all known bacteria that contain asparagine synthetase A form Asn-tRNA(Asn) by direct acylation catalyzed by asparaginyl-tRNA synthetase. Therefore, bacterial amide aminoacyl-tRNA formation is intimately tied to amide amino acid metabolism.
Sheppard
Kelly
K
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520-8114, USA.
Akochy
Pierre-Marie
PM
Salazar
Juan C
JC
Söll
Dieter
D
D000577:Amides / Q000737:chemistry; D000595:Amino Acid Sequence; D019881:Aminoacyltransferases / Q000737:chemistry; D002384:Catalysis; D004926:Escherichia coli / Q000378:metabolism; D005973:Glutamine / Q000737:chemistry*; D016480:Helicobacter pylori / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D019884:Nitrogenous Group Transferases / Q000235:genetics / Q000502:physiology*; D011550:Pseudomonas aeruginosa / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D012348:RNA, Transfer, Asn / Q000737:chemistry*; D012351:RNA, Transfer, Gln / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid
D000577:Amides; D012348:RNA, Transfer, Asn; D012351:RNA, Transfer, Gln; D005973:Glutamine; D012343:RNA, Transfer; D019881:Aminoacyltransferases; C023175:transamidases; C525150:GatCAB amidotransferase, Helicobacter pylori; D019884:Nitrogenous Group Transferases