A new cell line from larval fat bodies of the bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae).
A new cell line, designated IOZCAS-Ha-I, was initiated from the fat body of larvae of Helicoverpa armigera (Lepidoptera: Noctuidae) in TNM-FH medium containing 10% fetal bovine serum. Spherical cells were predominant among the various cell types. The cell line showed a typical lepidopteran chromosome pattern ranging from 58 to 239 chromosomes in the majority of the cells. It was confirmed to have originated from the H. armigera by the DNA amplification- fingerprinting polymerase chain reaction (DAF-PCR) technique. The new cell line was only slightly susceptible to the multiple nucleocapsid nuclear polyhedrosis viruses (NPV) from H. armigera.
Zhang
Huan
H
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China.
Zhang
Yong-An
YA
Qin
Qilian
Q
Wang
Yuzhu
Y
Li
Xuan
X
Miao
Lin
L
Yin
Zhenxian
Z
Zhang
Aijun
A
Qu
Liangjian
L
Ding
Cui
C
D000818:Animals; D002460:Cell Line; D002875:Chromosomes / Q000235:genetics; D004247:DNA / Q000032:analysis; D005216:Fat Body / Q000166:cytology*; D002479:Inclusion Bodies; D007814:Larva / Q000166:cytology; D007915:Lepidoptera / Q000166:cytology*
D004247:DNA