Isolation and identification of two xylanase-producing extremely alkali-tolerant strains of Bacillus halodurans from Turpan in China.
Bacillus halodurans XJU-1 and XJU-80 were characterized in terms of physiological and biochemical characteristics, and 16S rDNA sequence homology and DNA-DNA hybridization analysis. The two isolates can grow in nutrient broth at a broad range of pH values from 4.5 to 12.6 for XJU-1 and from 3.8 to 12.8 for XJU-80, respectively. And the optimum temperature of growth were around 39 degrees C and 42 degrees C, respectively. Phylogenetic analysis of the two strains based on comparison of 16S rRNA sequence revealed that they are closely related to Bacillus halodurans C-125 and DSM497Twith 99% identity. DNA-DNA hybridization showed that the highest levels of DNA-DNA relatedness were found between the two strains (85%) and the B. halodurans type strains (81.3% and 71.5%), respectively. Moreover, the G + C content of the genomic DNA was 40.5 mol% for XJU-1 and 42.2 mol% for XJU-80.Our results demonstrate that strains XJU-1 and XJU-80 should be classified as two new members of the species B. halodurans.
Yi
Xia
X
College of Life Science and Technology, Xinjiang University, Urumqi 830046, China. yixia0110@163.com
Xie
Zhou-jie
ZJ
Deng
Ai-hua
AH
Wang
Ning
N
Erkin
Rahman
R
D001407:Bacillus / Q000145:classification / Q000201:enzymology / Q000302:isolation & purification* / Q000502:physiology; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D012336:RNA, Ribosomal, 16S; D043364:Endo-1,4-beta Xylanases