Determination of non-steroidal anti-inflammatory drugs in urine by combining an immobilized carboxylated carbon nanotubes minicolumn for solid-phase extraction with capillary electrophoresis-mass spectrometry.
Last years, the usefulness of the use of carbon nanotubes (CNTs) as sorbent material have been demonstrated for a wide variety of compounds. In this work, it has been demonstrated for first time that immobilized carboxylated single-walled carbon nanotubes (c-SWNTs) offer clear advantages over the use of CNTs. The higher adsorption capacity has been attributed to the special orientation of c-SWNTs molecules on the glass surface. The potential of this new sorbent was evaluated for the preconcentration of non-steroidal anti-inflammatory drugs (NSAIDs) from urine samples. Purified samples were analysed by capillary electrophoresis-mass spectrometry detection allowing the determination of 1.6 to 2.6 microg/L of NSAIDs with only 5 mL of sample. The precision of the method for the determination of real spiked urine samples ranged from 5.4 to 7.4% and the recoveries from 98.6 to 102.2%.
Suárez
B
B
Department of Analytical Chemistry, Marie Curie Building (Annex), Campus de Rabanales, University of Cordoba, E-14071 Cordoba, Spain.
Simonet
B M
BM
Cárdenas
S
S
Valcárcel
M
M
D000327:Adsorption; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000652:urine*; D002264:Carboxylic Acids / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007213:Indomethacin / Q000652:urine; D007660:Ketoprofen / Q000652:urine; D000432:Methanol / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry*; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014046:Tolmetin / Q000652:urine; D014556:Urine / Q000737:chemistry*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D002264:Carboxylic Acids; D037742:Nanotubes, Carbon; D007660:Ketoprofen; D014046:Tolmetin; D007213:Indomethacin; D000432:Methanol