title: Bradykinin-related peptides, including a novel structural variant, (Val1)-bradykinin, from the skin secretion of Guenther's frog, Hylarana guentheri and their molecular precursors.
abstract: Multiple bradykinin-related peptides including a novel bradykinin structural variant, (Val(1))-bradykinin, have been identified from the defensive skin secretion of Guenther's frog, Hylarana guentheri by a tandem mass spectrometry method. Subsequently, four different preprobradykinin cDNAs, which encoded multiple bradykinin copies and its structural variants, were consistently cloned from a skin derived cDNA library. These preprobradykinin cDNAs showed little structural similarity with mammalian kininogens and the kininogens from the skin of toads, but have regions that are highly conserved in the kininogens from another ranid frog, Odorrana schmackeri. Alignment of these preprobradykinins revealed that preprobradykinin 1, 2 and 3 may derive from a single gene by alternative exon splicing.
authors.affiliation: Institute of Biomedical Sciences, University of Ulster, Cromore Road, Coleraine BT52 1SA, Northern Ireland, UK.
authors.forename: Jianwu
authors.initials: J
authors.lastname: Zhou
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Bjourson
authors.forename: Daniel J M
authors.initials: DJ
authors.lastname: Coulter
authors.forename: Tianbao
authors.initials: T
authors.lastname: Chen
authors.forename: Chris
authors.initials: C
authors.lastname: Shaw
authors.forename: Martin
authors.initials: M
authors.lastname: O'rourke
authors.forename: David G
authors.initials: DG
authors.lastname: Hirst
authors.forename: Yany
authors.initials: Y
authors.lastname: Zhang
authors.forename: Pingfan
authors.initials: P
authors.lastname: Rao
authors.forename: Stephen
authors.initials: S
authors.lastname: McClean
mesh_terms: D000595:Amino Acid Sequence; D029845:Amphibian Proteins / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D001001:Anura / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001920:Bradykinin / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D007704:Kininogens / Q000737:chemistry; D008297:Male; D008969:Molecular Sequence Data; D009130:Muscle, Smooth / Q000187:drug effects; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D011498:Protein Precursors / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D017208:Rats, Wistar; D017422:Sequence Analysis, DNA; D012867:Skin / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D029845:Amphibian Proteins; D018076:DNA, Complementary; D007704:Kininogens; D010455:Peptides; D011498:Protein Precursors; D001920:Bradykinin