Sulphite oxidase as key enzyme for protecting plants against sulphur dioxide.
Sulphur dioxide (SO(2)) is known as a strongly damaging air pollutant. After conversion to sulphite in aqueous solution, it becomes a strong nucleophilic agent that attacks numerous compounds in the cell. Therefore, plants have developed a mechanism to control sulphite levels. Recently, we have cloned and characterized the enzyme sulphite oxidase (SO) from Arabidopsis thaliana. Yet, its physiological role remained unclear. Here, we describe results demonstrating that SO is essential for detoxifying excessive amounts of sulphite in the cell which is important for the survival of the plant. T-DNA-tagged A. thaliana plants lacking the enzyme showed a decrease in vitality during SO(2) fumigation and a change in their S-metabolites. The same was found with RNA-interference (RNAi) plants that were generated for tobacco. On the contrary, over-expression of SO helped the plant to survive SO(2) concentrations that are detrimental for non-transformed wild-type (WT) plants, as was shown with poplar plants which are known to be particularly sensitive to SO(2). Fumigation induced the expression of the enzyme as demonstrated by promoter-reporter gene fusion, by immunoblot analysis of SO-protein and by induction of enzyme activity. This implies that SO, as an otherwise constitutively expressed protein, is under additional control by SO(2) in the environment.
Lang
Christina
C
Institut für Pflanzenbiologie, Technische Universität Braunschweig, Humboldtstrasse 1, D-38106 Braunschweig, Germany.
Popko
Jennifer
J
Wirtz
Markus
M
Hell
Rüdiger
R
Herschbach
Cornelia
C
Kreuzwieser
Jürgen
J
Rennenberg
Heinz
H
Mendel
Ralf R
RR
Hänsch
Robert
R
D000220:Adaptation, Biological; D017360:Arabidopsis / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D015536:Down-Regulation; D018506:Gene Expression Regulation, Plant; D005966:Glucuronidase / Q000032:analysis; D016297:Mutagenesis, Site-Directed; D010641:Phenotype; D032107:Populus / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D034622:RNA Interference; D050876:Sulfite Oxidase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D013458:Sulfur Dioxide / Q000494:pharmacology*; D014026:Nicotiana / Q000187:drug effects / Q000201:enzymology / Q000235:genetics
D013458:Sulfur Dioxide; D050876:Sulfite Oxidase; D005966:Glucuronidase