title: Intravitreal injection of ciliary neurotrophic factor (CNTF) causes peripheral remodeling and does not prevent photoreceptor loss in canine RPGR mutant retina.
abstract: Ciliary neurotrophic factor (CNTF) rescues photoreceptors in several animal models of retinal degeneration and is currently being evaluated as a potential treatment for retinitis pigmentosa in humans. This study was conducted to test whether CNTF prevents photoreceptor cell loss in XLPRA2, an early onset canine model of X-linked retinitis pigmentosa caused by a frameshift mutation in RPGR exon ORF15. Four different treatment regimens of CNTF were tested in XLPRA2 dogs. Under anesthesia, the animals received at different ages an intravitreal injection of 12 microg of CNTF in the left eye. The right eye served as a control and was injected with a similar volume of phosphate buffered saline (PBS). Ocular examinations were performed regularly during the treatment periods. At termination, the dogs were euthanatized, eyes collected and the retinas were processed for embedding in optimal cutting temperature (OCT) medium. The outer nuclear layer (ONL) thickness was evaluated on H&E sections and values in both CNTF- and PBS-treated eyes were compared. Morphologic alterations in the peripheral retina were characterized by immunohistochemistry using cell-specific markers. Cell proliferation in the retinas was examined on semi-thin plastic sections, and by BrdU pulse-labeling and Ki67 immunohistochemistry on cryosections. All CNTF-treated eyes showed early clinical signs of corneal epitheliopathy, subcapsular cataracts and uveitis. No statistically significant difference in ONL thickness was seen between the CNTF- and PBS-injected eyes. Prominent retinal remodeling that consisted in an abnormal increase in the number of rods, and in misplacement of some rods, cones, bipolar and Müller cells, was observed in the peripheral retina of CNTF-treated eyes. This was only seen when CNTF was in injected before the age at which the canine retina reaches full maturation. In XLPRA2 dogs, intravitreal injections of CNTF failed to prevent photoreceptors from undergoing cell death in the central and mid-peripheral retina. CNTF also caused ocular side-effects and morphologic alterations in the periphery that were consistent with cell dedifferentiation and proliferation. Our findings suggest that some inherited forms of retinal degeneration may not respond to CNTF's neuroprotective effects.
authors.affiliation: Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA. wbeltran@vet.upenn.edu
authors.forename: William A
authors.initials: WA
authors.lastname: Beltran
authors.forename: Rong
authors.initials: R
authors.lastname: Wen
authors.forename: Gregory M
authors.initials: GM
authors.lastname: Acland
authors.forename: Gustavo D
authors.initials: GD
authors.lastname: Aguirre
mesh_terms: D000818:Animals; D002386:Cataract / Q000139:chemically induced; D002452:Cell Count; D016923:Cell Death / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D020934:Ciliary Neurotrophic Factor / Q000008:administration & dosage* / Q000009:adverse effects; D004195:Disease Models, Animal; D004285:Dogs; D019573:Epithelium, Corneal / Q000187:drug effects; D008040:Genetic Linkage / Q000235:genetics; D007150:Immunohistochemistry / Q000379:methods; D007267:Injections; D009154:Mutation; D017956:Photoreceptor Cells, Invertebrate / Q000187:drug effects*; D012160:Retina / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D017949:Retinal Cone Photoreceptor Cells / Q000187:drug effects; D012162:Retinal Degeneration / Q000473:pathology / Q000503:physiopathology; D017948:Retinal Rod Photoreceptor Cells / Q000187:drug effects; D012174:Retinitis Pigmentosa / Q000188:drug therapy* / Q000235:genetics / Q000503:physiopathology; D014605:Uveitis / Q000139:chemically induced; D014822:Vitreous Body
chemical_list: D020934:Ciliary Neurotrophic Factor