Use of hematopoietic progenitor cell count on the Sysmex XE-2100 for peripheral blood stem cell harvest monitoring.
Successful peripheral blood stem cell (PBSC) collection depends on the timing of apheresis based on CD34+ cell enumeration. Because this analysis is expensive and induces organization difficulties, we evaluated hematopoietic progenitor cell (HPC) quantification on the Sysmex XE-2100 as a surrogate analysis. We tested 157 blood samples for CD34+ cells and HPC counts. We found a good linear correlation between HPC and CD34+ and determined simple rules allowing to use HPC count in daily practice. We set a positive cut-off >30 HPC/mm(3) for allowing PBSC harvest and a negative cut-off at 0 HPC/mm(3) for which collection should be delayed. These two situations accounted for 62% of cases and CD34+ cell count by flow cytometry confirmed HPC result in 95% of cases. Between 0 and 30 HPC/mm3, CD34+ enumeration is required for decision-making. We conclude that HPC count may be a useful surrogate for CD34+ enumeration in PBSC harvest monitoring.
Letestu
Rémi
R
Laboratoire Central d'Hématologie, Hôpital Necker Enfants Malades, Paris, France. remi.letestu@avc.ap-hop-paris.fr
Marzac
Christophe
C
Audat
Françoise
F
Belhocine
Ramdane
R
Tondeur
Sylvie
S
Baccini
Véronique
V
Garçon
Loïc
L
Cortivo
Liliane Dal
LD
Perrot
Jean-Yves
JY
Lefrère
François
F
Valensi
Françoise
F
Ajchenbaum-Cymbalista
Florence
F
D018952:Antigens, CD34 / Q000032:analysis / Q000097:blood; D001772:Blood Cell Count / Q000295:instrumentation*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D012680:Sensitivity and Specificity; D020858:Tissue and Organ Harvesting
D018952:Antigens, CD34