Gene variants of monocyte chemoattractant protein 1 and components of metabolic syndrome in KORA S4, Augsburg.
OBJECTIVE
Monocyte chemoattractant protein 1 (MCP-1) has been suggested to be involved in the development of several components of metabolic syndrome (MetS). The present study investigated the association of nine MCP-1 single nucleotide polymorphisms (SNPs) with MetS, type 2 diabetes mellitus and metabolic risk factors.


METHODS
The population-based study sample comprised 1630 subjects aged 55-74 years from KORA S4 (Cooperative Health Research in the Region of Augsburg Survey 4). Genotyping was carried out by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) analysis of allele-dependent primer extension products.


RESULTS
The MCP-1 SNP c.-3813C>T exhibited trends for differences between the genotype groups in triglycerides, 2-h glucose and uric acid (P = 0.0084, 0.014, 0.027). Other trends were observed for c.-928G>C associated with height and fasting glucose (P = 0.0024, 0.033), for c.105T>C with height and leukocytes (P = 0.0095, 0.047), for c.*65C>T and c.*3879C>T with MCP-1 levels (both P = 0.012) and for c.-2138A>T with interleukin-6 levels. After correction for multiple testing, none of the analysed SNPs, except c.-928G>C in men showed a significant association with MetS, T2DM or other analysed parameters. Haplotype MCP-1*1 and c.-928G>C in men (P = 0.0002, 0.0004) were significantly associated with an increase in height.


CONCLUSIONS
This is the first study to investigate the associations of MCP-1 SNPs with MetS. We found trends for several components of MetS. These parameters were hyperlipidaemia, fasting and 2-h glucose, and uric acid. A new finding is that MCP-1*1 haplotype is associated with height. Further investigation in larger populations is needed to clarify the involvement of MCP-1 in MetS.
Sedlmeier
Eva-Maria
EM
Institute of Epidemiology, GSF National Research Centre for Environment and Health, Ingolstädter Landstrasse 1, D-85764 Neuherberg, Germany.
Grallert
Harald
H
Huth
Cornelia
C
Löwel
Hannelore
H
Herder
Christian
C
Strassburger
Klaus
K
Giani
Guido
G
Wichmann
H-Erich
HE
Hauner
Hans
H
Illig
Thomas
T
Rathmann
Wolfgang
W
D000368:Aged; D001786:Blood Glucose / Q000032:analysis; D001827:Body Height / Q000235:genetics; D018932:Chemokine CCL2 / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000379:methods; D005215:Fasting; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D005858:Germany; D006239:Haplotypes; D006306:Health Surveys; D006801:Humans; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D024821:Metabolic Syndrome / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001786:Blood Glucose; D018932:Chemokine CCL2; D015850:Interleukin-6