title: Gene gun-delivered pGM-CSF adjuvant induces enhanced emigration of two dendritic cell subsets from the skin.
abstract: Two subsets of sheep afferent lymph dendritic cells (DC) are defined by the differential expression of CD172a and CD45RA. The majority (~70%) of CD172a(+) subset is CD45RA/CD11c(+)/CD207(+)/TLR4(+). The CD172a(-) DC are CD45RA(+)/CD207(-) and express low levels of CD11c and CD86. Real-time RT-PCR showed that CD172(+) DC produce IL-1beta and IL-10 and high levels of IL-18 but almost no IL-12p40; CD172a(-) DC express IL-12p40 but no IL-10 and low levels of IL-1beta and IL-18. Gene gun-delivered granulocyte-macrophage colony-stimulating factor (pGM-CSF) caused an early rise in the output of CD172a(+) DC, changes to DC phenotype and significant increases in the levels of expression cytokine transcripts. However, pGM-CSF did not affect any qualitative changes to cytokine expression, CD172a(+) DC remained IL-10(+)/IL-12p40(-) and the CD172(-) DC remained IL-10(-)/IL-12p40(+).
authors.affiliation: Centre for Infectious Diseases, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, UK.
authors.forename: K
authors.initials: K
authors.lastname: Matthews
authors.forename: S L
authors.initials: SL
authors.lastname: Bailey
authors.forename: A G
authors.initials: AG
authors.lastname: Gossner
authors.forename: C
authors.initials: C
authors.lastname: Watkins
authors.forename: R G
authors.initials: RG
authors.lastname: Dalziel
authors.forename: J
authors.initials: J
authors.lastname: Hopkins
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D019470:Biolistics; D002465:Cell Movement / Q000276:immunology*; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology; D005434:Flow Cytometry; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000008:administration & dosage*; D008196:Lymph / Q000166:cytology; D010641:Phenotype; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012756:Sheep; D012867:Skin / Q000166:cytology*
chemical_list: D000276:Adjuvants, Immunologic; D016207:Cytokines; D016178:Granulocyte-Macrophage Colony-Stimulating Factor