Activation of crystalline cellulose to cellulose III(I) results in efficient hydrolysis by cellobiohydrolase.
The crystalline polymorphic form of cellulose (cellulose I(alpha)-rich) of the green alga, Cladophora, was converted into cellulose III(I) and I(beta) by supercritical ammonium and hydrothermal treatments, respectively, and the hydrolytic rate and the adsorption of Trichoderma viride cellobiohydrolase I (Cel7A) on these products were evaluated by a novel analysis based on the surface density of the enzyme. Cellobiose production from cellulose III(I) was more than 5 times higher than that from cellulose I. However, the amount of enzyme adsorbed on cellulose III(I) was less than twice that on cellulose I, and the specific activity of the adsorbed enzyme for cellulose III(I) was more than 3 times higher than that for cellulose I. When cellulose III(I) was converted into cellulose I(beta) by hydrothermal treatment, cellobiose production was dramatically decreased, although no significant change was observed in enzyme adsorption. This clearly indicates that the enhanced hydrolysis of cellulose III(I) is related to the structure of the crystalline polymorph. Thus, supercritical ammonium treatment activates crystalline cellulose for hydrolysis by cellobiohydrolase.
Igarashi
Kiyohiko
K
Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, University of Tokyo, Japan.
Wada
Masahisa
M
Samejima
Masahiro
M
D000327:Adsorption; D000641:Ammonia / Q000737:chemistry; D002384:Catalysis; D002475:Cellobiose / Q000737:chemistry / Q000378:metabolism; D002482:Cellulose / Q000737:chemistry* / Q000378:metabolism; D043366:Cellulose 1,4-beta-Cellobiosidase / Q000737:chemistry* / Q000378:metabolism; D000460:Chlorophyta / Q000737:chemistry; D006358:Hot Temperature; D006868:Hydrolysis; D007700:Kinetics; D008958:Models, Molecular; D012972:Sodium Hydroxide / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D014242:Trichoderma / Q000201:enzymology; D014867:Water / Q000737:chemistry
D014867:Water; D002475:Cellobiose; D012972:Sodium Hydroxide; D000641:Ammonia; D002482:Cellulose; D043366:Cellulose 1,4-beta-Cellobiosidase