Seed defensins from T. kiharae and related species: genome localization of defensin-encoding genes.
Ten new defensins have been isolated from seeds of Triticum kiharae and related species of the Triticum and Aegilops genera by a combination of chromatographic procedures including affinity-, size-exclusion, and reversed-phase high-performance liquid chromatography. Nine were completely sequenced and shown to represent a family of closely related peptides with highly conserved amino acid sequences. Analysis of defensin compositions in diploid A-, B-, and D-genome donors to polyploid wheat allowed us for the first time to assign most defensin-encoding genes to particular hexaploid wheat genomes.
Odintsova
Tatyana I
TI
Laboratory of Plant Genetics, Vavilov Institute of General Genetics, Russian Academy of Sciences, ul. Gubkina 3, 117809 Moscow, Russian Federation.
Egorov
Tsezi A
TA
Musolyamov
Alexander Kh
AKh
Odintsova
Margarita S
MS
Pukhalsky
Vitaliy A
VA
Grishin
Eugene V
EV
D000595:Amino Acid Sequence; D002845:Chromatography; D023082:Defensins / Q000235:genetics*; D017343:Genes, Plant; D018745:Genome, Plant; D010940:Plant Proteins; D012639:Seeds / Q000276:immunology*; D014908:Triticum / Q000235:genetics / Q000276:immunology*
D023082:Defensins; D010940:Plant Proteins