title: Brominated lipids identify lipid binding sites on the surface of the reaction center from Rhodobacter sphaeroides.
abstract: This study describes the use of brominated phospholipids to distinguish between lipid and detergent binding sites on the surface of a typical alpha-helical membrane protein. Reaction centers isolated from Rhodobacter sphaeroides were cocrystallized with added brominated phospholipids. X-ray structural analysis of these crystals has revealed the presence of two lipid binding sites from the characteristic strong X-ray scattering from the bromine atoms. These results demonstrate the usefulness of this approach to mapping lipid binding sites at the surface of membrane proteins.
authors.affiliation: Department of Chemistry, WestCHEM.
authors.forename: Aleksander W
authors.initials: AW
authors.lastname: Roszak
authors.forename: Alastair T
authors.initials: AT
authors.lastname: Gardiner
authors.forename: Neil W
authors.initials: NW
authors.lastname: Isaacs
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Cogdell
mesh_terms: D001665:Binding Sites; D001966:Bromine; D003460:Crystallization; D018360:Crystallography, X-Ray; D020404:Glycerophospholipids / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry*; D008958:Models, Molecular; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry*; D012242:Rhodobacter sphaeroides / Q000737:chemistry* / Q000378:metabolism
chemical_list: D020404:Glycerophospholipids; D008565:Membrane Proteins; D045322:Photosynthetic Reaction Center Complex Proteins; D001966:Bromine