[Isolation and characterization of two aniline-degrading strains and compare of functional genes].
Two aniline-degrading bacterial strains, AN30 and DN425, were isolated from activated sludge of textile-printing wastewater treatment plant and identified as Pseudomonas sp. and Shewanella sp. , respectively. Under shaking condition, 250 mg/L aniline was removed 96.1% and 13.8% within 72h by strains AN30 and DN425, respectively. Under static condition, their degrading rates were 39.6% and 8.6% , respectively. Under static condition, the decolorizing rate of strain DN425 reached 96% for azo dye Great Red GR in 4h, exhibiting a remarkable color removal capability. However, strain AN30 was not capable of decolorizing Great Red GR. With two sets of specific primers for tdnQ gene and fre gene, the two genes were detected by PCR amplification. The results indicated the two strains possess both tdnQ gene and fre gene.
Ren
Sui-zhou
SZ
Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou 510070, China. rensz@163.com
Guo
Jun
J
Zeng
Guo-qu
GQ
Cen
Ying-hua
YH
Sun
Guo-ping
GP
D000814:Aniline Compounds / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011549:Pseudomonas / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology; D020592:Shewanella / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D000814:Aniline Compounds; D001426:Bacterial Proteins; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D014874:Water Pollutants, Chemical; C023650:aniline