title: Seed defensins from T. kiharae and related species: genome localization of defensin-encoding genes.
abstract: Ten new defensins have been isolated from seeds of Triticum kiharae and related species of the Triticum and Aegilops genera by a combination of chromatographic procedures including affinity-, size-exclusion, and reversed-phase high-performance liquid chromatography. Nine were completely sequenced and shown to represent a family of closely related peptides with highly conserved amino acid sequences. Analysis of defensin compositions in diploid A-, B-, and D-genome donors to polyploid wheat allowed us for the first time to assign most defensin-encoding genes to particular hexaploid wheat genomes.
authors.affiliation: Laboratory of Plant Genetics, Vavilov Institute of General Genetics, Russian Academy of Sciences, ul. Gubkina 3, 117809 Moscow, Russian Federation.
authors.forename: Tatyana I
authors.initials: TI
authors.lastname: Odintsova
authors.forename: Tsezi A
authors.initials: TA
authors.lastname: Egorov
authors.forename: Alexander Kh
authors.initials: AKh
authors.lastname: Musolyamov
authors.forename: Margarita S
authors.initials: MS
authors.lastname: Odintsova
authors.forename: Vitaliy A
authors.initials: VA
authors.lastname: Pukhalsky
authors.forename: Eugene V
authors.initials: EV
authors.lastname: Grishin
mesh_terms: D000595:Amino Acid Sequence; D002845:Chromatography; D023082:Defensins / Q000235:genetics*; D017343:Genes, Plant; D018745:Genome, Plant; D010940:Plant Proteins; D012639:Seeds / Q000276:immunology*; D014908:Triticum / Q000235:genetics / Q000276:immunology*
chemical_list: D023082:Defensins; D010940:Plant Proteins