title: [Expression and subcellular localization of XIAP and XAF1 in human normal oral keratinocytes and Tca8113 cells].
abstract: OBJECTIVE
  To clarify expression and subcellular localization of XIAP and XAF1 protein in human normal oral keratinocytes (hNOK) and Tca8113 cells human tongue carcinoma cell line.
  
  
  METHODS
  The hNOKs and Tca8113 cells were cultured in vitro. Expression and subcellular localization of XIAP and XAF1 protein were examined by combination of indirect immunofluorescence and confocal laser scanning microscopy.
  
  
  RESULTS
  XIAP expression was weak in the hNOKs and fluorescence staining localized chiefly in the cytoplasm and perinuclear areas. In the Tca8113 cells, high level of XIAP protein could be detected in both the cytoplasm and the nucleus. In the hNOKs, XAF1 distributed mostly in the nucleus. Homogeneous nuclear and cytoplasmic distribution of XAF1 could be visualized in the Tca8113 cells.
  
  
  CONCLUSIONS
  In cancerization of oral mucosa, XIAP protein could play an important antiapoptotic role by overexpression, while XAF1 protein does not appear to antagonize effectively the role of XIAP.
authors.affiliation: Department of Oral Mucosal Diseases, School of Stomatology, Jilin University, Changchun 130041, China. gaowenxin01@sina.com
authors.forename: Wen-xin
authors.initials: WX
authors.lastname: Gao
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
authors.forename: Xiu-feng
authors.initials: XF
authors.lastname: Wei
authors.forename: Ying-xin
authors.initials: YX
authors.lastname: Chen
authors.forename: Juan
authors.initials: J
authors.lastname: Zhang
authors.forename: Lai-kuan
authors.initials: LK
authors.lastname: Zhu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D015603:Keratinocytes / Q000378:metabolism* / Q000473:pathology; D009061:Mouth Mucosa / Q000166:cytology* / Q000378:metabolism; D009363:Neoplasm Proteins / Q000378:metabolism*; D014062:Tongue Neoplasms / Q000378:metabolism* / Q000473:pathology; D051636:X-Linked Inhibitor of Apoptosis Protein / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009363:Neoplasm Proteins; D051636:X-Linked Inhibitor of Apoptosis Protein; C417551:XAF1 protein, human; C496866:XIAP protein, human