title: Inhibitory effect of phosphoenolpyruvate on glycolytic enzymes in Escherichia coli.
abstract: For analyzing the control of energy metabolism in Escherichia coli, we carried out kinetic analyses of glycolytic enzymes purified from the overexpressing clones of E. coli K12 W3110 that were constructed with the vector pCA24N. Phosphoenolpyruvate (PEP) acted as an effective inhibitor of enzymes of the preparatory phase in glycolysis. Glucokinase was potently inhibited by PEP in a competitive manner with respect to ATP: the K(i) value for PEP was 0.1mM. PEP further inhibited phosphoglucoisomerase to a lesser extent, and phosphofructokinase A and aldolase A with 10-fold the K(i) values of glucokinase and phosphoglucoisomerase. Glucose is incorporated into E. coli through two pathways: the PTS (PEP-dependent phosphotransferase system) and the glucokinase reaction. PEP, a potent inhibitor of E. coli glucokinase, unlike most eukaryotic hexokinases, can act as a signal molecule controlling glucose uptake and glycolytic flux in cells.
authors.affiliation: Department of Biochemistry, Aichi Medical University School of Medicine, Nagakute, Aichi 480-1195, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Ogawa
authors.forename: Hirotada
authors.initials: H
authors.lastname: Mori
authors.forename: Masaru
authors.initials: M
authors.lastname: Tomita
authors.forename: Masataka
authors.initials: M
authors.lastname: Yoshino
mesh_terms: D048168:Escherichia coli K12 / Q000378:metabolism*; D005634:Fructose-Bisphosphate Aldolase / Q000037:antagonists & inhibitors; D005941:Glucokinase / Q000037:antagonists & inhibitors*; D006019:Glycolysis; D010728:Phosphoenolpyruvate / Q000494:pharmacology*; D010770:Phosphotransferases / Q000037:antagonists & inhibitors
chemical_list: D010728:Phosphoenolpyruvate; D010770:Phosphotransferases; D005941:Glucokinase; D005634:Fructose-Bisphosphate Aldolase