In vitro characterization of primary SIVsmm isolates belonging to different lineages. In vitro growth on rhesus macaque cells is not predictive for in vivo replication in rhesus macaques.
We report in vitro characterization of 11 SIVsmm strains of six lineages co-circulating in naturally infected sooty mangabeys (SMs) from US Primate Centers and showed no major differences in the in vitro replication pattern between different SIVsmm lineages. Primary SIVsmm isolates utilized CCR5 and Bonzo co-receptors in vitro. SIVsmm growth in human T cell lines was isolate-, not lineage-specific, with poor replication on Molt4-Clone8, CEMss and PM1 cells and better replication on MT2, SupT1 and CEMx174 cells. All primary SIVsmm isolates replicated on SM and human PBMCs. In vitro replication in macaques varied widely, with moderate to high replication in pig-tailed macaque PBMCs, enhanced by CD8+ T cell depletion, and highly variable replication on rhesus macaque (Rh) PBMCs. Primary SIVsmm isolates replicated in Rh monocyte-derived dendritic cells (MDDCs) and monocyte-derived macrophages (MDMs). In vivo, SIVsmm isolates replicated at high levels in all SIVsmm-infected Rh. The poor in vitro replication of primary SIVsmm isolates in Rh cells did not correlate with in vivo replication, emphasizing the value of in vivo studies.
Gautam
Rajeev
R
Division of Microbiology, Tulane National Primate Research Center, Covington, LA 70433, USA.
Carter
Anders Chase
AC
Katz
Nathalia
N
Butler
Isolde F
IF
Barnes
Mary
M
Hasegawa
Atsuhiko
A
Ratterree
Marion
M
Silvestri
Guido
G
Marx
Preston A
PA
Hirsch
Vanessa M
VM
Pandrea
Ivona
I
Apetrei
Cristian
C
D000818:Animals; D002460:Cell Line; D002469:Cell Separation; D002478:Cells, Cultured; D016665:Cercocebus atys; D003713:Dendritic Cells / Q000821:virology; D015683:Gene Products, gag / Q000097:blood; D006801:Humans; D008253:Macaca mulatta; D008264:Macrophages / Q000821:virology; D012367:RNA, Viral / Q000097:blood; D019713:Receptors, CCR5 / Q000378:metabolism; D011991:Receptors, Virus / Q000378:metabolism; D016097:Simian Acquired Immunodeficiency Syndrome / Q000821:virology; D015302:Simian Immunodeficiency Virus / Q000145:classification / Q000254:growth & development* / Q000302:isolation & purification / Q000472:pathogenicity*; D016176:T-Lymphocyte Subsets / Q000821:virology; D013601:T-Lymphocytes / Q000821:virology; D014481:United States; D019562:Viral Load; D014779:Virus Replication
C066594:Gag protein p27, Simian immunodeficiency virus; D015683:Gene Products, gag; D012367:RNA, Viral; D019713:Receptors, CCR5; D011991:Receptors, Virus