Impairment of innate immune responses of airway epithelium by infection with bovine viral diarrhea virus.
Bovine viral diarrhea virus (BVDV) infection is an important risk factor for development of shipping fever pneumonia in feedlot cattle, and infects but does not cause morphologic evidence of damage to airway epithelial cells. We hypothesized that BVDV predisposes to bacterial pneumonia by impairing innate immune responses in airway epithelial cells. Primary cultures of bovine tracheal epithelial cells were infected with BVDV for 48 h, then stimulated with LPS for 16 h. Expression of tracheal antimicrobial peptide (TAP) and lingual antimicrobial peptide (LAP) mRNA was measured by quantitative RT-PCR, and lactoferrin concentrations were measured in culture supernatant by ELISA. BVDV infection had no detectable effect on the constitutive expression of TAP and LAP mRNA or lactoferrin concentration in culture supernatant. LPS treatment provoked a significant increase in TAP mRNA expression and lactoferrin concentration in the culture supernatant (p<0.01), and these effects were significantly (p<0.02, p<0.01) abrogated by prior infection of the tracheal epithelial cells with the type 2 ncp-BVDV isolate. In contrast, infection with the type 1 ncp-BVDV isolate had no effect on TAP mRNA expression or lactoferrin secretion. LPS treatment induced a significant (p<0.001) upregulation of LAP mRNA expression, which was not significantly affected by prior infection with BVDV. These data indicate that infection with a type 2 BVDV isolate inhibits the LPS-induced upregulation of TAP mRNA expression and lactoferrin secretion by tracheal epithelial cells, suggesting a novel mechanism by which this virus abrogates respiratory innate immune responses and predisposes to bacterial pneumonia in cattle.
Al-Haddawi
M
M
Department of Pathobiology, University of Guelph, Guelph, Ontario, Canada N1G 2W1.
Mitchell
G B
GB
Clark
M E
ME
Wood
R D
RD
Caswell
J L
JL
D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics; D001483:Base Sequence; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000150:complications / Q000276:immunology; D002417:Cattle; D002478:Cells, Cultured; D017931:DNA Primers / Q000235:genetics; D030241:Diarrhea Virus 1, Bovine Viral / Q000276:immunology / Q000472:pathogenicity*; D030242:Diarrhea Virus 2, Bovine Viral / Q000276:immunology / Q000472:pathogenicity*; D004847:Epithelial Cells / Q000276:immunology / Q000378:metabolism / Q000821:virology; D015870:Gene Expression; D007113:Immunity, Innate; D007781:Lactoferrin / Q000235:genetics / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D012766:Pasteurellosis, Pneumonic / Q000209:etiology / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012307:Risk Factors; D014132:Trachea / Q000166:cytology / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D023083:beta-Defensins / Q000235:genetics
D023181:Antimicrobial Cationic Peptides; D017931:DNA Primers; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D023083:beta-Defensins; C092480:lingual antimicrobial peptide; C068629:tracheal antimicrobial peptide, bovine; D007781:Lactoferrin