Molecular cloning and expression analysis of a new gene for short-chain dehydrogenase/reductase 9.
We report here the cloning and characterization of a novel human short-chain dehydrogenases/reductase gene SCDR9, isolated from a human liver cDNA library, and mapped to 4q22.1 by browsing the UCSC genomic database. SCDR9 containing an ORF with a length of 900 bp, encoding a protein with a signal peptide sequence and an adh_short domain. GFP localization shows SCDR9 protein concentrated in some site of the cytoplasm, but not in the ER. Expression pattern in eighteen tissues revealed that SCDR9 is expressed highly in liver. Soluble recombinant protein was successfully purified from Escherichia coli using pET28A(+) expression vector. Our data provides important information for further study of the function of the SCDR9 gene and its products.
Liu
Shen
S
The Institute of Genetics, Fudan University, Shanghai, People's Republic of China.
Huang
Chaoqun
C
Li
Dawei
D
Ren
Weihua
W
Zhang
Haoxing
H
Qi
Meiyan
M
Li
Xin
X
Yu
Long
L
D015067:17-Hydroxysteroid Dehydrogenases; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002894:Chromosomes, Human, Pair 4; D003001:Cloning, Molecular; D006801:Humans; D008099:Liver / Q000201:enzymology; D051379:Mice; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000235:genetics*; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D016415:Sequence Alignment
D011994:Recombinant Proteins; D010088:Oxidoreductases; D015067:17-Hydroxysteroid Dehydrogenases; C000626699:HSD17B13 protein, human; D044925:Oxidoreductases Acting on CH-CH Group Donors