Protective action of indapamide, a thiazide-like diuretic, on ischemia-induced injury and barrier dysfunction in mouse brain microvascular endothelial cells.
The aim of the present study was to elucidate the effects of indapamide on ischemic damage to the blood-brain barrier (BBB) in vitro. The ischemia/reperfusion conditions employed here significantly decreased the viability of mouse brain capillary endothelial (MBEC4) cells, an effect ameliorated by indapamide. Ischemia increased the permeability of MBEC4 cells to two cellular transport markers, sodium fluorescein and Evan's blue-albumin. Indapamide reduced the ischemia-induced hyperpermeability of cells. These results suggest that indapamide may have a protective role against ischemia-induced injury and dysfunction of the BBB.
Nishioku
Tsuyoshi
T
Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, Japan.
Takata
Fuyuko
F
Yamauchi
Atsushi
A
Sumi
Noriko
N
Yamamoto
Ikumi
I
Fujino
Akiko
A
Naito
Mikihiko
M
Tsuruo
Takashi
T
Shuto
Hideki
H
Kataoka
Yasufumi
Y
D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects* / Q000503:physiopathology; D001921:Brain / Q000098:blood supply* / Q000187:drug effects; D002545:Brain Ischemia / Q000188:drug therapy* / Q000503:physiopathology; D002199:Capillary Permeability / Q000187:drug effects; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D002540:Cerebral Cortex / Q000098:blood supply / Q000187:drug effects; D004396:Coloring Agents / Q000494:pharmacology; D004232:Diuretics / Q000494:pharmacology*; D042783:Endothelial Cells / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D005070:Evans Blue / Q000494:pharmacology; D019793:Fluorescein; D005456:Fluorescent Dyes; D007190:Indapamide / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D015427:Reperfusion Injury / Q000188:drug therapy / Q000503:physiopathology / Q000517:prevention & control
D004396:Coloring Agents; D004232:Diuretics; D005456:Fluorescent Dyes; D005070:Evans Blue; D007190:Indapamide; D019793:Fluorescein