Molecular polymorphism of O alleles in the Chinese Han population.
The ABO blood group system is the most important in transfusion medicine. O blood group is common in Chinese Han people, but the distribution of various O alleles is unknown. Sequences of exon6 and exon7 of the O allele at the ABO gene locus were studied in 100 individuals of the O phenotype randomly selected from the Chinese Han population. Some samples, when required, were cloned and sequenced spanning exon6 and exon7. Eight O alleles were found in the Chinese population. Most have the 2 common O01 or O02 alleles. The allele frequency of ABO*O01 was 0.47, and that of ABO*O02 was 0.495. One individual was found to have O05 allele. Five alleles were found to differ from all alleles reported to date. Four of these alleles differed from either the O01 allele (1 out of 4) or O02 allele (3 out of 4) by 1 point mutation at A468G, G489A, T526C, or T1104G. The fifth allele differed from the O01 allele since it does not have nt261G deletion but has C467T mutation. This novel allele occurred in 2 individuals. O genetic analysis suggests that the O01 allele prevails, with O1v accounting for about 97% of these in the Chinese Han population. The O03 allele that has been shown to occur with a frequency of <5% in other populations was not detected. But the novel O allele without 261G deletion has been found in Chinese for the first time. Surely more O alleles will be found in the Chinese population.
Yang
Bao-Cheng
BC
ShenZhen Blood Center, Ni-Gang Xi Road, Mei-Gang Nan Street, ShenZhen, Guangdong, P.R. China.
Zeng
Jian-Qiang
JQ
Yu
Qiong
Q
Liang
Yan-Lian
YL
Su
Yu-Qing
YQ
Deng
Zhi-Hui
ZH
D000017:ABO Blood-Group System / Q000235:genetics*; D044466:Asian People / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers; D005091:Exons / Q000235:genetics; D005787:Gene Frequency; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
D000017:ABO Blood-Group System; D017931:DNA Primers