title: Expression of Escherichia coli heat-labile enterotoxin b subunit (LTB) in carrot (Daucus carota L.).
abstract: We expressed the B subunit of enterotoxigenic Escherichia coli heat-labile enterotoxin (LTB) encoded by a synthetic codon-optimized gene in carrot. An Agrobacterium-mediated transformation method was used. Thirty independent transgenic lines were regenerated via somatic embryogenesis after 6 months in culture and were transferred to a greenhouse. GM1-ELISA assay was used to assess LTB protein content in mature taproots. Some transgenic lines expressed LTB up to 0.3% of the total soluble protein, which is tenfold higher than the expression levels reported earlier using the native bacterial gene in plants. Immunological assay confirmed proper assembly of the pentameric complex and in vitro activity of the recombinant LTB protein, suggesting that it can be functional in prevention of diarrhea.
authors.affiliation: División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José 2055, 78216 San Luis Potosí, S.L.P., Mexico.
authors.forename: Sergio
authors.initials: S
authors.lastname: Rosales-Mendoza
authors.forename: Ruth Elena
authors.initials: RE
authors.lastname: Soria-Guerra
authors.forename: María Teresa
authors.initials: MT
authors.lastname: de Jesús Olivera-Flores
authors.forename: Rubén
authors.initials: R
authors.lastname: López-Revilla
authors.forename: Gerardo Rafael
authors.initials: GR
authors.lastname: Argüello-Astorga
authors.forename: Juan Francisco
authors.initials: JF
authors.lastname: Jiménez-Bremont
authors.forename: Ramón Fernando
authors.initials: RF
authors.lastname: García-de la Cruz
authors.forename: Juan Pablo
authors.initials: JP
authors.lastname: Loyola-Rodríguez
authors.forename: Angel Gabriel
authors.initials: AG
authors.lastname: Alpuche-Solís
mesh_terms: D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism*; D018552:Daucus carota / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D004768:Enterotoxins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D030821:Plants, Genetically Modified
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C027538:heat-labile enterotoxin, E coli