[Expression of Daxx in children with acute leukemia].
OBJECTIVE
To investigate Daxx expression and its clinical significance in children with acute leukemia.


METHODS
The expression of Daxx protein was detected by immunohistochemical assay in 50 children with newly diagnosed acute leukemia (34 cases of acute lymphocytic leukemia and 16 cases of acute non-lymphocytic leukemia). Twenty children with normal bone marrow were used as the control group.


RESULTS
Daxx protein was expressed in 38.0% of 50 children with acute leukemia, which was significantly higher than that of the control group (5.0%) (P < 0.05). The children with acute non-lymphocytic leukemia had significantly higher Daxx expression levels (62.5%) than those with acute lymphocytic leukemia (26.5%; P < 0.05) as well as the control group (P < 0.05). There were no significant differences in the Daxx expression between acute lymphocytic leukemia children and the control group. Daxx protein was expressed in 55.6% of high risk group of acute lymphocytic leukemia but it was not expressed in standard risk group of acute lymphocytic leukemia (P < 0.05).


CONCLUSIONS
Daxx expression is abnormal in children with acute leukemia and associated with some clinical features of acute leukemia, suggesting that it may play an important role in the genesis and development of acute leukemia.
Liu
Jing
J
Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. liujing821218@sohu.com
Zhang
Liu-Qing
LQ
Hu
Qun
Q
Lin
Han-Hua
HH
Liu
Ai-Guo
AG
Tao
Hong-Fang
HF
Song
Yan-Qing
YQ
Zhang
Xiao-Ling
XL
D048868:Adaptor Proteins, Signal Transducing / Q000032:analysis*; D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D056970:Co-Repressor Proteins; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy / Q000378:metabolism*; D008297:Male; D018832:Molecular Chaperones; D016328:NF-kappa B / Q000378:metabolism; D009687:Nuclear Proteins / Q000032:analysis*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000188:drug therapy / Q000378:metabolism*
D048868:Adaptor Proteins, Signal Transducing; D056970:Co-Repressor Proteins; C106985:DAXX protein, human; D018832:Molecular Chaperones; D016328:NF-kappa B; D009687:Nuclear Proteins