Rab 5 is required for the cellular entry of dengue and West Nile viruses.
The mechanisms of cellular entry of dengue and West Nile viruses are not well characterized. We show that both these viruses enter HeLa cells by clathrin-dependent endocytosis and require vacuolar acidic pH. Inhibition of the GTPase Rab 5 or 7, which regulates transport to early or late endosomes, respectively, demonstrated that Rab 5 was essential for survival of both dengue and West Nile virus. These data broaden our understanding of the pathways required for productive dengue and West Nile virus infection and may facilitate new strategies for combating disease.
Krishnan
Manoj N
MN
Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, The Anlyan Center for Medical Research and Education, 300 Cedar Street, New Haven, CT 06520-8031, USA.
Sukumaran
Bindu
B
Pal
Utpal
U
Agaisse
Herve
H
Murray
James L
JL
Hodge
Thomas W
TW
Fikrig
Erol
E
D003716:Dengue Virus / Q000378:metabolism*; D004705:Endocytosis / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D034622:RNA Interference; D014617:Vacuoles / Q000737:chemistry / Q000378:metabolism; D053586:Virus Internalization; D014902:West Nile virus / Q000378:metabolism*; D020696:rab5 GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
D049452:Green Fluorescent Proteins; D020696:rab5 GTP-Binding Proteins