Anti-tumor effects of Rubratoxin B on cell toxicity, inhibition of cell proliferation, cytotoxic activity and matrix metalloproteinase-2,9.
Terrestrial fungi are a prolific source of metabolites with significant biological activities. Many important anticancer, antifungal and antibacterial chemotherapeutics are either microbial metabolites or semisynthetic derivatives. Investigating the metabolites of fungi may increase the chance of finding novel compounds. A bioassay-guided fractionation of soil fungus Penicillium purpurogenum fermentation, yielded the metabolite Rubratoxin B using morphological deformation of Pyricularia oryzae mycelia. Analysis by flow cytometry showed that Rubratoxin B inhibited the cell cycle progression of tsFT210 cells in the G2/M phase at the concentration of 24 microM. Cytotoxic activity analysis showed that Rubratoxin B has a cytotoxic activity and IC50 was 67.3 +/- 1.4 microM on human fibrosarcoma cell (HT1080), and Rubratoxin B exhibited inhibitory activities against matrix metalloproteinase (MMP)-2 and 9 on HT 1080 cells with an inhibitory rate of 61.5% and 74.7% at 30 microM, respectively.
Wang
Tao
T
Department of Pharmacognosy, Kyoto Pharmaceutical University, Kyoto 607-8412, Japan. wangtao@poppy.kyoto-phu.ac.jp
Zhang
Yi
Y
Wang
Yi
Y
Pei
Yue-hu
YH
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D061965:Matrix Metalloproteinase Inhibitors; D025282:Mycelium / Q000737:chemistry / Q000187:drug effects / Q000648:ultrastructure; D009183:Mycotoxins / Q000494:pharmacology*; D010407:Penicillium / Q000737:chemistry / Q000254:growth & development; D012988:Soil Microbiology; D013778:Tetrazolium Salts; D013844:Thiazoles
D000970:Antineoplastic Agents; D061965:Matrix Metalloproteinase Inhibitors; D009183:Mycotoxins; D013778:Tetrazolium Salts; D013844:Thiazoles; C100297:rubratoxins; C022616:thiazolyl blue