title: The plant alkaloid sanguinarine is a potential inhibitor of follicular angiogenesis.
abstract: Sanguinarine (SA), a phytobiotic from Sanguinaria Canadensis, has been demonstrated to inhibit vessel growth. Current restrictions on the use of antibiotic growth promoters have motivated addition of this alkaloid as a naturally appetizing feed additive for farm animals. However, concern may araise since angiogenesis is a fundamental event in ovarian follicle growth. Therefore, the aim of this study was to evaluate the potential negative role of SA in follicular angiogenesis. For this purpose, we studied the effect of 300 nM SA on the production of vascular endothelial growth factor (VEGF) by swine granulosa cells from follicles >5 mm and on the activation of Akt, the main effector of the VEGF signalling pathway. In addition, the potential interference of SA in vessel development was tested in an in vitro angiogenesis bioassay. SA inhibited both VEGF production and VEGF-induced Akt activation in swine granulosa cells. Moreover, it was able to block vessel growth induced by VEGF. Taken together, our results suggest that SA could be detrimental to follicular angiogenesis, and therefore supplementation of feed with this alkaloid should be carefully considered.
authors.affiliation: Department of Animal Production, Veterinary Biotechnology, Food Safety and Quality-Veterinary Physiology, University of Parma, Italy. basini@unipr.it
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Basini
authors.forename: Suyen Eleonora
authors.initials: SE
authors.lastname: Santini
authors.forename: Simona
authors.initials: S
authors.lastname: Bussolati
authors.forename: Francesca
authors.initials: F
authors.lastname: Grasselli
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D053119:Benzophenanthridines / Q000494:pharmacology*; D002478:Cells, Cultured; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D007546:Isoquinolines / Q000494:pharmacology*; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D006080:Ovarian Follicle / Q000098:blood supply*; D010936:Plant Extracts / Q000494:pharmacology; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D031681:Sanguinaria / Q000737:chemistry; D013552:Swine; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D000470:Alkaloids; D000890:Anti-Infective Agents; D053119:Benzophenanthridines; D007546:Isoquinolines; D010936:Plant Extracts; D042461:Vascular Endothelial Growth Factor A; C005705:sanguinarine; D051057:Proto-Oncogene Proteins c-akt