title: Sensitive and specific detection of Xanthomonas campestris pv. vesicatoria by PCR using pathovar-specific primers based on rhs family gene sequences.
abstract: The present study describes PCR assay to detect bacterial spot caused by Xanthomonas campestris pv. vesicatoria in pepper and tomato. One set of PCR primer was developed to amplify gene required for an rhs family gene homologous to rhsA, cell envelope biogenesis, outer membrane. Only a PCR product of a 517bp was produced in PCR reaction with the Xanthomonas campestris pv. vesicatoria (XCVF/XCVR) primer set. A specific, and highly sensitive and rapid PCR assay for the detection of X. campestris pv. vesicatoria was achieved. The protocol can be used as a reliable diagnostic tool for specific detection of X. campestris pv. vesicatoria in pepper or tomato.
authors.affiliation: National Institute of Agricultural Biotechnology, Rural Development Administration, 441-707 Suwon, Republic of Korea. dspark@rda.go.kr
authors.forename: Dong Suk
authors.initials: DS
authors.lastname: Park
authors.forename: Jae Kyung
authors.initials: JK
authors.lastname: Shim
authors.forename: Jung Sun
authors.initials: JS
authors.lastname: Kim
authors.forename: Chun Keun
authors.initials: CK
authors.lastname: Lim
authors.forename: Rosemary
authors.initials: R
authors.lastname: Shrestha
authors.forename: Jang Ho
authors.initials: JH
authors.lastname: Hahn
authors.forename: Hong Gi
authors.initials: HG
authors.lastname: Kim
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D002212:Capsicum / Q000382:microbiology; D017931:DNA Primers / Q000235:genetics*; D018551:Solanum lycopersicum / Q000382:microbiology; D005810:Multigene Family; D010935:Plant Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity; D016959:Xanthomonas campestris / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers