title: Type III collagen is essential for growth acceleration of human osteoblastic cells by ascorbic acid 2-phosphate, a long-acting vitamin C derivative.
abstract: Collagen has been reported to be essential for the proliferation of various kinds of cells including human osteoblastic cells [Takamizawa, S., Maehata, Y., Imai, K., Senoo, H., Sato, S., Hata, R., 2004. Effects of ascorbic acid and ascorbic acid 2-phosphate, a long-acting vitamin C derivative, on the proliferation and differentiation of human osteoblast-like cells. Cell Biol. Int. 28, 255-265], but the type(s) of collagen responsible for growth regulation is not known. Presently we found that ascorbic acid 2-phosphate, a long-acting vitamin C derivative, stimulated both cell growth and the expression of mRNA for type III collagen in human osteoblast-like MG-63 cells and in normal human osteoblasts, as well as in human bone marrow mesenchymal stem cells, but not the expression of type I collagen in these cells. Epidermal growth factor also stimulated both cell growth and expression of type III collagen mRNA in MG-63 cells. Among MG-63 cell clones, their growth rates correlated significantly with their COL3A1 messenger RNA levels but not with their COL1A1 or COL1A2 messenger RNA levels. Transfection of MG-63 cells with siRNA for COL3A1 but not with that for COL1A1 decreased the growth rates of the transfected cells concomitant with a drop in the level of COL3A1 mRNA. Furthermore, cell proliferation as observed by thymidine incorporation into DNA and cell number was increased when MG-63 cells were cultured on type III collagen-coated dishes. Taken together, our results indicate that type III collagen is the collagen component responsible for the growth stimulation of human osteoblastic cells.
authors.affiliation: Department of Biochemistry and Molecular Biology, Kanagawa Dental College, Yokosuka, Japan.
authors.forename: Yojiro
authors.initials: Y
authors.lastname: Maehata
authors.forename: Shinji
authors.initials: S
authors.lastname: Takamizawa
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Ozawa
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Izukuri
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Kato
authors.forename: Sadao
authors.initials: S
authors.lastname: Sato
authors.forename: Masaichi-Chang-Il
authors.initials: MC
authors.lastname: Lee
authors.forename: Akinori
authors.initials: A
authors.lastname: Kimura
authors.forename: Ryu-Ichiro
authors.initials: R
authors.lastname: Hata
mesh_terms: D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D024061:Collagen Type III / Q000235:genetics / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005786:Gene Expression Regulation; D006801:Humans; D010006:Osteoblasts / Q000187:drug effects / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism
chemical_list: D024061:Collagen Type III; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D004815:Epidermal Growth Factor; D001205:Ascorbic Acid