title: Quantitative demonstration of intrathecal synthesis of high affinity immunoglobulin G in herpes simplex encephalitis using affinity-mediated immunoblotting.
abstract: Three paired serial samples of CSF and serum (from days 8, 13 and 22) were taken from a patient referred to the National Hospital for Neurology and Neurosurgery with what was duly confirmed as having herpes simplex encephalitis using PCR. The samples were investigated using affinity-mediated immunoblotting followed by incubation with sodium thiocyanate. Digitisation of the blots enabled further analysis. We showed that the clones of antigen-specific IgG, which were produced intrathecally, were of higher relative affinity than polyclonal antigen-specific IgG.
authors.affiliation: Department of Neuroimmunology, Institute of Neurology, Queen Square, London, WC1N 3BG, UK. m.chapman@ion.ucl.ac.uk
authors.forename: Miles D
authors.initials: MD
authors.lastname: Chapman
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Thompson
authors.forename: Paul M
authors.initials: PM
authors.lastname: Candler
authors.forename: Russell C
authors.initials: RC
authors.lastname: Dale
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Church
authors.forename: Gavin
authors.initials: G
authors.lastname: Giovannoni
mesh_terms: D000368:Aged; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000097:blood / Q000134:cerebrospinal fluid*; D000915:Antibody Affinity; D019540:Area Under Curve; D004279:DNA, Viral; D020803:Encephalitis, Herpes Simplex / Q000134:cerebrospinal fluid* / Q000276:immunology; D005260:Female; D018259:Herpesvirus 1, Human / Q000276:immunology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D015151:Immunoblotting / Q000379:methods*; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood / Q000134:cerebrospinal fluid*; D016133:Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D007074:Immunoglobulin G