title: TRPV1 mediates histamine-induced itching via the activation of phospholipase A2 and 12-lipoxygenase.
abstract: Histamine provokes itching and is a major skin disease complaint. Histamine is known to excite a subset of sensory neurons, predominantly C-fibers. Although histamine is pruritogenic, its signaling pathways that excite sensory neurons have not been identified. Because the metabolic products of lipoxygenases (LOs) activate transient receptor potential vanilloid receptor-1 (TRPV1) in sensory neurons, we hypothesized that histamine excites sensory neurons by activating TRPV1 via phospholipase A2 (PLA2) and LO stimulation. In cultured sensory neurons, histamine evoked inward currents that were reduced by capsazepine, a TRPV1 blocker. Moreover, histamine provoked inward currents when histamine receptor subtype 1 (H1R) and TRPV1 were expressed heterologously, but not when H1R or TRPV1 was expressed alone. In addition, histamine caused Ca2+ influxes in sensory neurons in wild-type mice but not in TRPV1-/- mice. Furthermore, histamine caused a 2.5-fold increase in the production of 12-hydroxyeicosatetraenoic acid, a metabolite of LO, in cultured sensory neurons. When injected subcutaneously into the necks of mice, histamine caused bouts of scratching, which were greatly reduced by pretreatment with capsazepine, a TRPV1 blocker, and by inhibitors of PLA2, LO, and H1R. Furthermore, mice lacking TRPV1 markedly reduced histamine-induced scratching compared with wild type. Together, these results indicate that TRPV1 plays a key role in mediating the pruritogenic action of histamine via the PLA2/LO pathway.
authors.affiliation: Sensory Research Center, College of Pharmacy, Seoul National University, Seoul 151-742, Korea.
authors.forename: Won-Sik
authors.initials: WS
authors.lastname: Shim
authors.forename: Min-Ho
authors.initials: MH
authors.lastname: Tak
authors.forename: Mi-Hyun
authors.initials: MH
authors.lastname: Lee
authors.forename: Minjung
authors.initials: M
authors.lastname: Kim
authors.forename: Minkyung
authors.initials: M
authors.lastname: Kim
authors.forename: Jae-Yeon
authors.initials: JY
authors.lastname: Koo
authors.forename: Chang-Hun
authors.initials: CH
authors.lastname: Lee
authors.forename: Misook
authors.initials: M
authors.lastname: Kim
authors.forename: Uhtaek
authors.initials: U
authors.lastname: Oh
mesh_terms: D000818:Animals; D001092:Arachidonate 12-Lipoxygenase / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002211:Capsaicin / Q000031:analogs & derivatives / Q000494:pharmacology; D002478:Cells, Cultured; D004789:Enzyme Activation; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006632:Histamine / Q000378:metabolism / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000378:metabolism; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D051381:Rats; D050916:TRPV Cation Channels / Q000037:antagonists & inhibitors / Q000502:physiology*
chemical_list: D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse; C493922:Trpv1 protein, rat; D006632:Histamine; D001092:Arachidonate 12-Lipoxygenase; D010741:Phospholipases A; D054467:Phospholipases A2; C071423:capsazepine; D002211:Capsaicin; D002118:Calcium