A novel cold-inducible expression system for Bacillus subtilis.
Production of recombinant proteins at low temperatures is one strategy to prevent formation of protein aggregates and the use of an expensive inducer such as IPTG. We report on the construction of two expression vectors both containing the cold-inducible des promoter of Bacillus subtilis, where one allows intra- and the other extracellular synthesis of recombinant proteins. Production of recombinant proteins started within the first 30min after temperature downshock to 25 degrees C and continued for about 5h.
Thuy Le
Ai Thi
AT
Institute of Genetics, Universität Bayreuth, D-95440 Bayreuth, Germany.
Schumann
Wolfgang
W
D001412:Bacillus subtilis / Q000235:genetics*; D001483:Base Sequence; D003080:Cold Temperature; D004269:DNA, Bacterial / Q000235:genetics; D015870:Gene Expression; D050939:Gene Fusion; D005798:Genes, Bacterial; D017930:Genes, Reporter; D005822:Genetic Vectors; D007763:Lac Operon; D008969:Molecular Sequence Data; D009876:Operon; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D000516:alpha-Amylases / Q000096:biosynthesis / Q000235:genetics; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
D004269:DNA, Bacterial; D011994:Recombinant Proteins; D000516:alpha-Amylases; D001616:beta-Galactosidase