title: Induced parthenogenesis in mandarin for haploid production: induction procedures and genetic analysis of plantlets.
abstract: This study focused on haploid induction in mandarin through in situ gynogenesis by pollination with irradiated pollen of 'Meyer' lemon. Pollination was carried out for three genotypes of mandarin with four levels of gamma-ray-irradiated pollen (150, 300, 600, and 900 Gy). The resulting seeds were characterised by a small size. Embryos were rescued in vitro and the ploidy level of the plantlets was determined by flow cytometry analysis. Haploid, diploid, triploid plantlets were obtained. The haploid parthenogenetic origin was confirmed using microsatellite marker analysis and chromosome count. Diploid and triploid plants were the result of crosses between mandarin and lemon. The induction of gynogenetic haploids of 'Fortune' (Citrus clementina Hort ex Tan. x Citrus tangerina Hort ex Tan.) and 'Ellendale' (Citrus reticulata Blanco x Citrus sinensis L. Osb) is reported here for the first time.
authors.affiliation: Unité de Recherche Multiplication végétative, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), San Giuliano 20230, France. froelicher@cirad.fr
authors.forename: Yann
authors.initials: Y
authors.lastname: Froelicher
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Bassene
authors.forename: Emna
authors.initials: E
authors.lastname: Jedidi-Neji
authors.forename: Dominique
authors.initials: D
authors.lastname: Dambier
authors.forename: Raphael
authors.initials: R
authors.lastname: Morillon
authors.forename: Geneviève
authors.initials: G
authors.lastname: Bernardini
authors.forename: Gilles
authors.initials: G
authors.lastname: Costantino
authors.forename: Patrick
authors.initials: P
authors.lastname: Ollitrault
mesh_terms: D002957:Citrus / Q000235:genetics* / Q000254:growth & development*; D004307:Dose-Response Relationship, Radiation; D005638:Fruit / Q000254:growth & development; D018525:Germination; D006238:Haploidy; D010312:Parthenogenesis / Q000235:genetics*; D011058:Pollen / Q000528:radiation effects; D012639:Seeds / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects