Quantitative analysis of Tenecteplase in rat plasma samples using LC-MS/MS as an alternative for ELISA.
An LC-MS/MS method has been developed for the quantitative determination of a protein drug (Tenecteplase; M(W) 58,777 Da) in rat plasma. The protein was digested with trypsin without prior clean-up of the plasma sample, without the use of a label nor internal standard. A limited validation was performed to assess the linearity, the sensitivity and the specificity of the method. In addition, the developed method was applied to the quantitative analysis of Tenecteplase in rat plasma samples originating from a single-dose study in rats.
Buscher
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BA
Business Unit Analytical Research, TNO Quality of Life, P.O. Box 360, 3700 AJ Zeist, The Netherlands. brigitte.buscher@tno.nl
Gerritsen
H
H
van Schöll
I
I
Cnubben
N H P
NH
Brüll
L P
LP
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D005343:Fibrinolytic Agents / Q000097:blood*; D051381:Rats; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000077785:Tenecteplase; D010959:Tissue Plasminogen Activator / Q000097:blood*
D005343:Fibrinolytic Agents; D010959:Tissue Plasminogen Activator; D000077785:Tenecteplase