Potential application of urea-derived herbicides as cytokinins in plant tissue culture.
Various urea-derived herbicides and different cytokinin analogues were used to determine their effects on callusing response and shoot regenerating capacity of alfalfa (Medicago sativa L.) and Coleus (Coleus forskohlii Briq.). The herbicides monuron and diuron evoked profuse callusing response from Coleus leaf segments and alfalfa petiole explants on Murashige and Skoog medium. Shoot regeneration by monuron (2.0 mg/l) showed a maximum of 3 multiple shoots both in alfalfa and Coleus with a frequency of 92% and 75%, respectively. Whereas diuron (0.5 mg/l) showed a high frequency of shoot regeneration (89%)with a mean number of 5 shoots in alfalfa, in C.forskohlii, the frequency of regeneration was 90%with a mean number of 6 shoots. Diuron with two chloride groups in the phenyl ring showed significantly higher cytokinin-like activity than single chloride substitution monuron. This study demonstrates the potential use of monuron and diuron as cytokinins in plant tissue culture.
Srinivasan
Malathi
M
Department of Biochemistry, Indian Institute of Science, Bangalore 560 012, India.
Nachiappan
Vasanthi
V
Rajasekharan
Ram
R
D031330:Coleus / Q000187:drug effects* / Q000502:physiology; D003583:Cytokinins / Q000494:pharmacology*; D004237:Diuron / Q000737:chemistry / Q000494:pharmacology*; D006540:Herbicides / Q000737:chemistry / Q000494:pharmacology*; D000455:Medicago sativa / Q000187:drug effects* / Q000502:physiology; D008782:Methylurea Compounds / Q000737:chemistry / Q000494:pharmacology*; D018520:Plant Shoots / Q000187:drug effects / Q000502:physiology; D012038:Regeneration; D046509:Tissue Culture Techniques
D003583:Cytokinins; D006540:Herbicides; D008782:Methylurea Compounds; D004237:Diuron; C100088:monuron