Mycobacterium seoulense sp. nov., a slowly growing scotochromogenic species.
A previously undescribed, slowly growing, scotochromogenic mycobacterium was isolated from a patient with symptomatic pulmonary infection during hsp65 sequence-based identification of Korean clinical isolates. Phenetic characteristics of this strain were generally similar to those of Mycobacterium nebraskense and Mycobacterium scrofulaceum. However, some phenetic characteristics differentiated it from these two species. Its 16S rRNA gene sequences were unique and phylogenetic analysis based on 16S rRNA gene sequences placed the organism in the slowly growing Mycobacterium group close to M. nebraskense and M. scrofulaceum. Its unique mycolic acid profiles and the results of phylogenetic analysis based on two independent alternative chronometer molecules, hsp65 and rpoB, confirmed the taxonomic status of this strain as representing a novel species. These data support the conclusion that this strain represents a novel mycobacterial species, for which the name Mycobacterium seoulense sp. nov. is proposed. The type strain is strain 03-19(T) (=DSM 44998(T)=KCTC 19146(T)).
Mun
Ho-Suk
HS
Department of Microbiology and Immunology, Cancer Research Institute and Liver Research Institute, College of Medicine, Seoul National University, Seoul 110-799, Korea.
Kim
Hyun-Ju
HJ
Department of Microbiology and Immunology, Cancer Research Institute and Liver Research Institute, College of Medicine, Seoul National University, Seoul 110-799, Korea.
Oh
Eun-Ju
EJ
Department of Microbiology and Immunology, Cancer Research Institute and Liver Research Institute, College of Medicine, Seoul National University, Seoul 110-799, Korea.
Kim
Hong
H
Department of Microbiology and Immunology, Cancer Research Institute and Liver Research Institute, College of Medicine, Seoul National University, Seoul 110-799, Korea.
Bai
Gil-Han
GH
The Korean Institute of Tuberculosis, The Korean National Tuberculosis Association, Seoul 137-140, Korea.
Yu
Hee-Kyung
HK
The Korean Institute of Tuberculosis, The Korean National Tuberculosis Association, Seoul 137-140, Korea.
Park
Young-Gil
YG
The Korean Institute of Tuberculosis, The Korean National Tuberculosis Association, Seoul 137-140, Korea.
Cha
Chang-Yong
CY
Department of Microbiology and Immunology, Cancer Research Institute and Liver Research Institute, College of Medicine, Seoul National University, Seoul 110-799, Korea.
Kook
Yoon-Hoh
YH
Department of Microbiology and Immunology, Cancer Research Institute and Liver Research Institute, College of Medicine, Seoul National University, Seoul 110-799, Korea.
Kim
Bum-Joon
BJ
Department of Microbiology and Immunology, Cancer Research Institute and Liver Research Institute, College of Medicine, Seoul National University, Seoul 110-799, Korea.
D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005260:Female; D006801:Humans; D008171:Lung Diseases / Q000382:microbiology*; D008875:Middle Aged; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics; D009164:Mycobacterium Infections / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S