[The pathogenetic role of peripheral T-lymphocytes activation-induced cell death in hemorrhagic fever with renal syndrome].
OBJECTIVE
To study the activation-induced cell death (AICD) in peripheral blood T-lymphocytes (PBL-T) from patients of hemorrhagic fever with renal syndrome (HFRS) and the effect of AICD on the pathogenesis of HFRS.


METHODS
The PBL-T of patients with HFRS were isolated by negative selection with magnetic beads and cultured with or without phytohemagglutinin (PHA) and ionomycin. The apoptotic ratio of PBL-T was detected with TUNEL staining and assessed with flow cytometry. The level of caspase-3 of PBL-T in patients with HFRS was detected with Western blot. Characteristic gene fragment of Hantaan virus and Seoul virus was detected with reverse transcription polymerase chain reaction.


RESULTS
In HFRS patients, the apoptotic ratio (27.79 +/- 0.99)% of PBL-T activated with PHA and ionomycin was significantly higher than that without activation (17.16 +/- 1.14)%, (P < 0.01). The apoptotic ratio of PBL-T in HFRS patients was higher than that of healthy control (P < 0.01), no matter with or without activation. The expression level of caspase-3 increased in the induced group of patients with HFRS and the expression level of 17 000 (the active component of caspase-3) increased significantly. The results of sequencing showed that amplification production from the serum are positive in 19 cases, seven being Hantaan virus and twelve Seoul virus.


CONCLUSIONS
AICD in PBL-T was found in patients of with HFRS. The high expression rate of caspase-3 in the PBL-T of patients with HFRS was related with AICD in the patients with HFRS. In the HFRS patients with specific IgM antibody positive, characteristic fragments can be obtained with RT-PCR from 79.2% patients. AICD of T lymphocytes may play an important role in the pathogenesis of HFRS.
Wang
Xiao-yan
XY
The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China.
Kang
Peng
P
Gao
Jie
J
Yu
Jian-wu
JW
Lü
Shu-lan
SL
D000328:Adult; D016022:Case-Control Studies; D053148:Caspase 3 / Q000096:biosynthesis*; D016923:Cell Death; D002460:Cell Line; D005260:Female; D005434:Flow Cytometry; D006480:Hemorrhagic Fever with Renal Syndrome / Q000209:etiology* / Q000276:immunology / Q000473:pathology; D006801:Humans; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000276:immunology*
D053148:Caspase 3