Dynamics modelling of biolistic gene guns.
The gene transfer process using biolistic gene guns is a highly dynamic process. To achieve good performance, the process needs to be well understood and controlled. Unfortunately, no dynamic model is available in the open literature for analysing and controlling the process. This paper proposes such a model. Relationships of the penetration depth with the helium pressure, the penetration depth with the acceleration distance, and the penetration depth with the micro-carrier radius are presented. Simulations have also been conducted. The results agree well with experimental results in the open literature. The contribution of this paper includes a dynamic model for improving and manipulating performance of the biolistic gene gun.
Zhang
Mingjun
M
Life Science and Chemical Analysis Division, Agilent Technologies, Santa Clara, CA 95051, USA. mingjunzhang@ieee.org
Tao
Weimin
W
Pianetta
Piero A
PA
D019470:Biolistics / Q000295:instrumentation* / Q000379:methods*; D003198:Computer Simulation; D004247:DNA / Q000008:administration & dosage*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D008954:Models, Biological
D004247:DNA