Isolation of a subtilisin-like serine protease gene (MyxSubtSP) from spores of Myxobolus cerebralis, the causative agent of whirling disease.
Proteases play important roles in parasite life cycles and host-parasite interactions. They are pathogenesis factors of many pathogenic organisms and are hence potential targets for chemotherapeutic treatment of disease. We identified a subtilisin-like serine protease gene, MyxSubtSP, expressed by Myxobolus cerebralis. After PCR with subtilisin-like serine protease primers, the gene was cloned, sequenced and aligned against the NCBI database. Its corresponding amino acid sequence included the putative conserved domains of Peptidase_S8, subtilase family and AprE, subtilisin-like serine proteases. Rapid amplification of 5' and 3' cDNA ends (RACE) was used to generate the full length (1385 bp) gene, with a 429 bp open reading frame. The gene encompasses coding regions for a catalytic triad formed by Asp-74, His-100 and Ser-110.
Dörfler
Christoph
C
Institute of Zoology, Fish Biology and Fish Diseases, University of Munich, Kaulbachstrasse 37, 80539 Munich, Germany.
El-Matbouli
Mansour
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D016054:DNA, Protozoan / Q000737:chemistry; D056890:Eukaryota / Q000201:enzymology* / Q000235:genetics* / Q000472:pathogenicity; D005393:Fish Diseases / Q000469:parasitology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011480:Protease Inhibitors / Q000494:pharmacology; D011529:Protozoan Infections, Animal / Q000469:parasitology*; D033761:Spores, Protozoan / Q000235:genetics; D020860:Subtilisin / Q000187:drug effects / Q000235:genetics*
D017931:DNA Primers; D016054:DNA, Protozoan; D011480:Protease Inhibitors; D020860:Subtilisin