Trypanosoma cruzi: involvement of glycoinositolphospholipids in the attachment to the luminal midgut surface of Rhodnius prolixus.
Trypanosoma cruzi epimastigotes adhere in vivo to the luminal surface of their triatomid vector digestive tract by molecular mechanisms, as yet, unknown. Here, we show that the administration of 0.5 microM epimastigote major surface glycoinositolphospholipids (GIPLs) to the infected bloodmeal inhibits up to 90% parasite infection in Rhodnius prolixus. The parasite behavior was investigated in vitro using fragments of the insect midgut. The addition of GIPLs in concentration as low as 50-100 nM impaired 95% the attachment of epimastigotes. Previous treatment of GIPLs with trifluoroacetic acid to remove the terminal beta-galactofuranosyl residues reversed 50% the epimastigote in vitro attachment. The binding sites of purified GIPLs on the luminal surface of the posterior midgut were exposed by immunofluorescence microscopy. These observations indicate that GIPLs are one of the components involved in the adhesion of T. cruzi to the luminal insect midgut surface and possibly one of the determinants of parasite infection in the insect vector.
Nogueira
Nadir F S
NF
Laboratório de Biologia Celular e Tecidual, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ, Brazil.
Gonzalez
Marcelo S
MS
Gomes
José Eugênio
JE
de Souza
Wanderley
W
Garcia
Eloi S
ES
Azambuja
Patricia
P
Nohara
Lilian L
LL
Almeida
Igor C
IC
Zingales
Bianca
B
Colli
Walter
W
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D014355:Chagas Disease / Q000469:parasitology / Q000635:transmission; D004305:Dose-Response Relationship, Drug; D006017:Glycolipids / Q000737:chemistry / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D007303:Insect Vectors / Q000469:parasitology*; D018613:Microscopy, Confocal; D018715:Microscopy, Video; D010743:Phospholipids / Q000737:chemistry / Q000502:physiology*; D011817:Rabbits; D012239:Rhodnius / Q000469:parasitology*; D021241:Spectrometry, Mass, Electrospray Ionization; D014349:Trypanosoma cruzi / Q000737:chemistry / Q000187:drug effects / Q000502:physiology*
D006017:Glycolipids; D010743:Phospholipids; C115336:glycoinositolphospholipid, Trypanosoma