title: Simultaneous use of multiple fluorescent reporter dyes for glucose sensing in aqueous solution.
abstract: The simultaneous use of several fluorescent reporter dyes in a multicomponent boronic acid-based glucose sensing system is reported. In one application, two dyes with widely different emission wavelengths are used to report changes in glucose concentration. A third glucose-insensitive dye was then added to act as a reference dye and provide for a ratiometric correction to the two reporter dye signals. The inclusion of such a reference dye reduces errors arising from sources such as fluctuations in lamp intensity and sample dilution.
authors.affiliation: Department of Chemistry and Biochemistry, University of California, Santa Cruz, 1156 High Street, Santa Cruz, CA 95064, USA.
authors.forename: David B
authors.initials: DB
authors.lastname: Cordes
authors.forename: Aaron
authors.initials: A
authors.lastname: Miller
authors.forename: Soya
authors.initials: S
authors.lastname: Gamsey
authors.forename: Bakthan
authors.initials: B
authors.lastname: Singaram
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D012996:Solutions; D014867:Water / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D012996:Solutions; D014867:Water; D005947:Glucose