The sympathetic postganglionic and sensory innervation of oviducal magnum in hen: a choleratoxin subunit B-conjugated horseradish peroxidase study.
The anatomy of the extrinsic innervation of the avian magnum has not been accurately demonstrated previously. In the present study, choleratoxin subunit B-conjugated horseradish peroxidase (CB-HRP) was used as a retrograde tracer to determine the sympathetic postganglionic and sensory innervation of the magnum of hens. With regard to the sympathetic postganglionic innervation, following CB-HRP injections under the serosa of the magnum, CB-HRP-positive neurons were found bilaterally in the C12-LS13 ganglia of the sympathetic chain, splanchnic ganglia and adrenal ganglia. The number of labelled neurons in the left ganglia of the sympathetic chain and splanchnic ganglia was approximately 2.1 times that in the right ganglia. This suggests that the unilateral magnum is bilaterally innervated with sympathetic postganglionic nerves, the left nerves being predominant. With regard to the sensory innervation, following tracer injections, CB-HRP-positive neurons were found bilaterally in the spinal ganglia C13-LS12, jugular ganglia and nodose ganglia. The number of positive cells in the left ganglia was about 2.2 times that in the right ganglia. In the spinal ganglia, 85.6% of the labelled neurons were in the T5-LS2 and LS8-LS11 ganglia. These results suggest that the sensory nerve fibres of the magnum reach the central nervous system principally via two groups of spinal ganglia and vagus nerves, and that the innervation is bilateral although the left-hand route predominates. Moreover, 45.7% of all the CB-HRP-labelled neurons were found in the rectal region of the intestinal nerve of Remak (INR), which suggests that the INR plays a very important role in the functional regulation of the magnum.
Liu
Jinxiong
J
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
Wang
Wenjie
W
Feng
Yamei
Y
Li
Meiying
M
Bao
Huijun
H
Chen
Qiusheng
Q
D000818:Animals; D001338:Autonomic Fibers, Postganglionic / Q000166:cytology*; D002645:Chickens / Q000033:anatomy & histology*; D002772:Cholera Toxin; D004396:Coloring Agents; D005260:Female; D006735:Horseradish Peroxidase; D009434:Neural Pathways / Q000502:physiology; D009475:Neurons, Afferent / Q000166:cytology*; D010057:Oviducts / Q000294:innervation*; D013116:Spinal Cord / Q000166:cytology; D013194:Staining and Labeling; D013564:Sympathetic Nervous System / Q000502:physiology*
D004396:Coloring Agents; C052635:cholera toxin, B subunit-horseradish peroxidase; D002772:Cholera Toxin; D006735:Horseradish Peroxidase