title: Identification and characterization of microRNAs from the bovine adipose tissue and mammary gland.
abstract: We report the identification of bovine miRNAs by cloning small RNAs from adipose tissue and the mammary gland. Fifty-nine distinct miRNAs were identified, five of them were not homologous to known mammalian miRNAs, and many of them had 3' and/or 5' end variants. Ribonuclease protection assays indicated that miR-23a and miR-24, whose genes are closely located on the same chromosome, were co-expressed in different tissues. The assays also suggested a role for several miRNAs in the mammary gland and a role for miR-133, a previously known skeletal and cardiac muscle-specific miRNA, in the rumen, an organ unique to the ruminant.
authors.affiliation: Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, 3130 Litton Reaves Hall, Blacksburg, VA 24061-0306, USA.
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Gu
authors.forename: Satyanaryana
authors.initials: S
authors.lastname: Eleswarapu
authors.forename: Honglin
authors.initials: H
authors.lastname: Jiang
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D015870:Gene Expression; D008321:Mammary Glands, Animal / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics* / Q000302:isolation & purification; D005810:Multigene Family; D012417:Rumen / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D018076:DNA, Complementary; D035683:MicroRNAs