title: Reconstitution of the biochemical activities of the AttJ repressor and the AttK, AttL, and AttM catabolic enzymes of Agrobacterium tumefaciens.
abstract: The attKLM operon encodes a lactonase (AttM) that hydrolyzes acylhomoserine lactone autoinducers, as well as two putative dehydrogenases (AttK and AttL). Here we show that AttK, AttL, and AttM collectively covert gamma-butyrolactone to succinate. Two metabolic intermediates, gamma-hydroxybutyrate and succinic semialdehyde, inactivated the AttJ repressor in vitro and induced attKLM transcription in vivo.
authors.affiliation: Department of Microbiology, 361A Wing Hall, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Yunrong
authors.initials: Y
authors.lastname: Chai
authors.forename: Ching Sung
authors.initials: CS
authors.lastname: Tsai
authors.forename: Hongbaek
authors.initials: H
authors.lastname: Cho
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Winans
mesh_terms: D015107:4-Butyrolactone / Q000378:metabolism*; D016960:Agrobacterium tumefaciens / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D002265:Carboxylic Ester Hydrolases / Q000235:genetics / Q000378:metabolism*; D018983:DNA Footprinting; D004269:DNA, Bacterial / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D017353:Gene Deletion; D008954:Models, Biological; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins / Q000502:physiology*; D019802:Succinic Acid / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D012097:Repressor Proteins; D019802:Succinic Acid; D010088:Oxidoreductases; D002265:Carboxylic Ester Hydrolases; C432003:N-acyl homoserine lactonase; D015107:4-Butyrolactone