title: Proteomic of lipid rafts in the exocrine pancreas from diet-induced obese rats.
abstract: In the present work, we induced obesity in rats with high-energy-starch diet and studied exocrine pancreas response. The zymogen granule (ZG) or purified plasma membrane (PM) from the exocrine pancreas was used for the isolation of the detergent-resistant membranes (DRMs). Based on high content of cholesterol, GM1, the bile salt dependent lipase (BSDL), and GP2 enrichment, the low-density fractions were defined as lipid rafts. Additionally, the rafts vesicles were determined by immunogold labeling with anti BSDL. By combining MALDI-TOF/MS and nano-LC ESI Q-TOF MS/MS proteomic identification we have selected 33 proteins from the lipid rafts which were classified into at least four functional families. Our data suggest that the acinar PM from the diet-induced obesity rats may be organized into lipid rafts, and characterization of rafts proteome can contribute to improve our understanding of food digestion under obesity.
authors.affiliation: Université Paul Cézanne, FST-Institut Méditerranéen de Recherche en Nutrition IMRN, UMR INRA 1111/PNV UMR 6153 1147, Marseille, France.
authors.forename: Amine Adda
authors.initials: AA
authors.lastname: Berkane
authors.forename: Hang Thi Thu
authors.initials: HT
authors.lastname: Nguyen
authors.forename: Fabrice
authors.initials: F
authors.lastname: Tranchida
authors.forename: Abdul A
authors.initials: AA
authors.lastname: Waheed
authors.forename: Valérie
authors.initials: V
authors.lastname: Deyris
authors.forename: Léopold
authors.initials: L
authors.lastname: Tchiakpe
authors.forename: Caroline
authors.initials: C
authors.lastname: Fasano
authors.forename: Cendrine
authors.initials: C
authors.lastname: Nicoletti
authors.forename: Véronique
authors.initials: V
authors.lastname: Desseaux
authors.forename: El Hassan
authors.initials: el H
authors.lastname: Ajandouz
authors.forename: Danielle
authors.initials: D
authors.lastname: Comeau
authors.forename: Louis
authors.initials: L
authors.lastname: Comeau
authors.forename: Abel
authors.initials: A
authors.lastname: Hiol
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000737:chemistry* / Q000648:ultrastructure; D004032:Diet; D008297:Male; D021962:Membrane Microdomains / Q000737:chemistry* / Q000378:metabolism; D009765:Obesity / Q000378:metabolism*; D046790:Pancreas, Exocrine / Q000737:chemistry* / Q000378:metabolism; D011506:Proteins / Q000032:analysis; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D011506:Proteins