title: Differentiation of Entamoeba histolytica and Entamoeba dispar by PCR: a preliminary study in Izmir, Turkey.
abstract: The causative agent of amoebiasis is currently attributed to two distinct species (E. histolytica and E. dispar). The aim of this study was to differentiate these species by PCR in stool samples. Isolated genomic DNA was amplified by PCR and band products of 101 bp (E. dispar) were obtained. All seven stool samples were found to be E. dispar, not E. histolytica. Our results demonstrated the significance of E. histolytica/dispar differentiation in the diagnosis of amoebiasis. This study is preliminary to our current research project entitled "Investigation of the prevalence of amoebiasis and Entamoeba species in Izmir and its hinterland".
authors.affiliation: Ege University School of Medicine, Department of Parasitology, Izmir, Turkey. hande.dagci@ege.edu.tr
authors.forename: Hande
authors.initials: H
authors.lastname: Dagci
authors.forename: Derya Dirim
authors.initials: DD
authors.lastname: Erdogan
authors.forename: Seray Ozensoy
authors.initials: SO
authors.lastname: Toz
authors.forename: Ozgur
authors.initials: O
authors.lastname: Kurt
authors.forename: Sebnem
authors.initials: S
authors.lastname: Ustun
authors.forename: Ulus
authors.initials: U
authors.lastname: Akarca
mesh_terms: D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis / Q000302:isolation & purification; D004747:Entamoeba / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004748:Entamoeba histolytica / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004749:Entamoebiasis / Q000175:diagnosis* / Q000469:parasitology*; D005243:Feces / Q000469:parasitology; D016133:Polymerase Chain Reaction / Q000379:methods*; D014421:Turkey
chemical_list: D016054:DNA, Protozoan