[Detection of anti-Sp17 antibodies in infertile patients' serum and its clinical significance].
OBJECTIVE
To test anti-Sp17 antibodies in the serum of AsAb positive infertile patients, to investigate the proportion of anti-Spl7 antibodies in AsAb and their potential application to the serologic diagnosis of immune infertility and immunocontraception.


METHODS
With human recombinant Sp17 as the antigen, the ELISA method was used to detect the positive rate, antibody titre and content of anti-Sp17 antibodies in the AsAb positive serum.


RESULTS
The positive rate of anti-Sp17 antibodies in the AsAb positive serum was 56.5%, with no significant difference in the gender aspect. The percentage of anti-Sp17 antibodies in AsAb was (10.09 +/-7.45) %, with statistical significance (P <0.05).


CONCLUSIONS
Sp17 is an important sperm antigen. Anti-Sp17 antibodies in the serum can be taken as auxiliary diagnostic index of infertility, and Sp17 is shown to be a potential candidate immunocontraception vaccine.
Zhang
Chun-hua
CH
PLA Institute of Clinical Laboratory Medicine, Nanjing General Hospital of Nanjing Command, PLA, Nanjing, Jiangsu 210002, China. zch198028@163.com
Li
Fang-qiu
FQ
Yang
Ai-long
AL
Sun
Wei
W
Miao
Jia-wen
JW
D000328:Adult; D000954:Antigens, Surface / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood*; D002148:Calmodulin-Binding Proteins; D002352:Carrier Proteins / Q000276:immunology*; D003269:Contraception, Immunologic; D005260:Female; D006801:Humans; D007248:Infertility, Male / Q000097:blood / Q000276:immunology*; D008297:Male; D008565:Membrane Proteins; D013094:Spermatozoa / Q000276:immunology
D000954:Antigens, Surface; D001323:Autoantibodies; D002148:Calmodulin-Binding Proteins; D002352:Carrier Proteins; D008565:Membrane Proteins; C090172:SPA17 protein, human