title: Cloning and characterization of a novel splice variant of human U2AF1L3 gene.
abstract: Pre-mRNA splicing allows individual genes to produce multiple protein isoforms with diverse functions. Recognition of functional splice sites in pre-mRNAs is very important in this splicing process and requires some protein auxiliary factors such as U2 small nuclear ribonucleoprotein auxiliary factor small subunit (U2AF35, encoded by U2AF1). By its RNA binding domains, U2AF35 interacts with U2AF65 to bind 3' splice site of pre-mRNA and initiates splicing. Another protein, which is named as U2AF1-like3 (U2AF1L3), shows high similarity with U2AF35 and may have related function in pre-mRNA splicing. Here, we report a splice variant of U2AF1L3, which is 767 bp in length and has an open reading frame (ORF) coding a predicted 181 amino acids protein. Reverse transcription-PCR (RT-PCR) shows that this isoform has different expression pattern with U2AF1L3 and is highly expressed in heart, brain and lung.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Chen
authors.forename: Chaoneng
authors.initials: C
authors.lastname: Ji
authors.forename: Tonghai
authors.initials: T
authors.lastname: Dou
authors.forename: Nan
authors.initials: N
authors.lastname: Zheng
authors.forename: Rui
authors.initials: R
authors.lastname: Qiu
authors.forename: Jing
authors.initials: J
authors.lastname: Peng
authors.forename: Weiqun
authors.initials: W
authors.lastname: Fang
authors.forename: Congjing
authors.initials: C
authors.lastname: Feng
authors.forename: Yi
authors.initials: Y
authors.lastname: Xie
authors.forename: Yumin
authors.initials: Y
authors.lastname: Mao
mesh_terms: D017398:Alternative Splicing / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D019295:Computational Biology; D017931:DNA Primers; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D022821:RNA Splice Sites / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012261:Ribonucleoproteins / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D000072668:Splicing Factor U2AF
chemical_list: D017931:DNA Primers; D009687:Nuclear Proteins; D022821:RNA Splice Sites; D012261:Ribonucleoproteins; D000072668:Splicing Factor U2AF; C486290:U2AF1L4 protein, human