title: Screening of rifamycin producing marine sponge bacteria by LC-MS-MS.
abstract: HPLC-MS-MS has been used for the identification and characterisation of rifamycin B and rifamycin SV in various strains of the marine sponge-derived bacterium Salinispora. Gradient elution using acetonitrile/water/ammonium acetate was used to separate the rifamycins from the matrix and negative ion-electrospray mass spectrometry was used for detection and confirmation. The presence of rifamycin in bacterial extracts was confirmed by matching retention times, parent ion spectra and the fragmentated parent ion spectra of the standard compounds and the bacterial extracts. All strains of the marine sponge bacterium Salinispora tested were found to contain rifamycin thus an alternate actinobacterial source of rifamycin was established.
authors.affiliation: School of Pharmacy, The University of Queensland, Brisbane, Queensland 4072, Australia. a.hewavitharana@pharmacy.uq.edu.au
authors.forename: Amitha K
authors.initials: AK
authors.lastname: Hewavitharana
authors.forename: P Nicholas
authors.initials: PN
authors.lastname: Shaw
authors.forename: Tae Kyung
authors.initials: TK
authors.lastname: Kim
authors.forename: John A
authors.initials: JA
authors.lastname: Fuerst
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006094:Gram-Positive Bacteria / Q000378:metabolism*; D011161:Porifera / Q000382:microbiology*; D012294:Rifamycins / Q000096:biosynthesis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D012294:Rifamycins