title: Crohn's disease and polymorphism of heat shock protein gene HSP70-2 in the Tunisian population.
abstract: OBJECTIVE
  Crohn's disease is a multifactorial disorder with a pivotal role of the genetic component. HSP70-2 gene, located in IBD3 region, has a PstI polymorphic site associated recently with Crohn's disease especially with a perforating form. In this study, we sought to determine whether this polymorphism was associated with Crohn's disease in the Tunisian population and its correlation with clinical manifestation of the disease.
  
  
  METHODS
  In all, 148 patients with Crohn's disease and 81 healthy individuals were genotyped for the HSP70-2 PstI polymorphism by restriction fragment length polymorphism analysis.
  
  
  RESULTS
  The allele and genotype frequency of the PstI polymorphism did not differ between patients and controls. Furthermore, this polymorphism was not associated with specific disease behavior.
  
  
  CONCLUSIONS
  This study reported the absence of association between Crohn's disease and HSP70-2 gene in the Tunisian population. The allele A of PstI polymorphism was not associated with phenotype of the disease.
authors.affiliation: Department of Gastroenterology, La Rabta Hospital, Tunis, Tunisia. w.mekki@laposte.net
authors.forename: Lilia
authors.initials: L
authors.lastname: Zouiten-Mekki
authors.forename: Sami
authors.initials: S
authors.lastname: Karoui
authors.forename: Maher
authors.initials: M
authors.lastname: Kharrat
authors.forename: Monia
authors.initials: M
authors.lastname: Fekih
authors.forename: Samira
authors.initials: S
authors.lastname: Matri
authors.forename: Jalel
authors.initials: J
authors.lastname: Boubaker
authors.forename: Azza
authors.initials: A
authors.lastname: Filali
authors.forename: Habiba
authors.initials: H
authors.lastname: Chaabouni
mesh_terms: D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D003424:Crohn Disease / Q000235:genetics* / Q000601:surgery; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D008297:Male; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D013997:Time Factors
chemical_list: D018840:HSP70 Heat-Shock Proteins; C091879:HSPA2 protein, human