[Recombinant hPR gene yeast for assessing in drinking water].
A bioassay method using recombinant human progesterone receptor (hPR) gene yeast to evaluate the environmental endocrine disrupter effects was introduced. This method was used to determine the inhibition of hPR activity in pollutants emitted from a water works in the south China. The result showed that the recombinant gene yeast had steroid specificity and dose-respond curve for progesterone with EC50 value of 0.5 nmol/L; The estrogenic chemicals pentachlorophenol and p-nonylphenol inhibited the activity of hPR in the yeast which had IC50 values of 2.4 micromol/L and 3.7 micromol/L, respectively. The results indicate that the recombinant progesterone gene yeast assay technique is an efficient and rapid method for screening and quantitatively analyzing the endocrine disrupters which can be the inhibitor of hPR activity. Combined with the solid-phase extraction (SPE) procedure, the yeast assay showed an obvious inhibition of hPR activity for water works sample and the inhibition rates were more than 58 %. Therefore, it is reliable to use the recombinant hPR gene yeast for assessing the environmental endocrine disrupters.
Li
Jian
J
State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Cui
Qing
Q
Ma
Mei
M
Rao
Kai-feng
KF
Wang
Zi-jian
ZJ
D001681:Biological Assay; D052244:Endocrine Disruptors / Q000032:analysis / Q000633:toxicity; D006801:Humans; D010416:Pentachlorophenol / Q000032:analysis* / Q000633:toxicity; D010636:Phenols / Q000032:analysis* / Q000633:toxicity; D011980:Receptors, Progesterone / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000633:toxicity; D014881:Water Supply / Q000032:analysis*; D015003:Yeasts / Q000187:drug effects / Q000235:genetics / Q000378:metabolism
D052244:Endocrine Disruptors; D010636:Phenols; D011980:Receptors, Progesterone; D011994:Recombinant Proteins; D014874:Water Pollutants, Chemical; C025256:nonylphenol; D010416:Pentachlorophenol