title: Revelation of simple and complex liver acini after portal transplantation of pancreatic islets or thyroid follicles in rats.
abstract: The microarchitecture of the liver is still not completely understood although various concepts of structural liver organization have been proposed. Among them, Rappaport's liver acinus stands out as one of the most accepted models. The correctness of this model, however, has also been doubted, and its applicability is hampered by the fact that the outlines of the liver acinus are disguised and nobody was ever able to give visual evidence by "unmasking" a simple liver acinus from the surrounding liver tissue. After intraportal transplantation of pancreatic islets or thyroid follicles into diabetic or thyroidectomized rats, respectively, the transplants engraft in small portal tracts and morphologically alter the downstream liver tissue due to excessive hormone secretion. Using a combined approach of perfusion fixation, stereomicroscopy, and light microscopy, we demonstrate in this study that these foci of altered liver tissue represent simple and complex liver acini, exactly as described by Rappaport. We present stereomicroscopical and histological examples of all important cut levels of altered simple and complex liver acini, including their topographical relation to the supplying and draining vessels and to the "central vein" liver lobule. Moreover, by computer-aided reconstruction of serial semi-thin sections, we were able to present the first 3-dimensional images of simple and complex liver acini.
  
  
  CONCLUSIONS
  Our results prove the correctness of Rappaport's acinus model and confirm the simple liver acinus as the principal microcirculatory unit of the liver.
authors.affiliation: Ernst-Moritz-Arndt-Universität, Greifswald, Germany. frank.dombrowski@uni-greifswald.de
authors.forename: Frank
authors.initials: F
authors.lastname: Dombrowski
authors.forename: Matthias
authors.initials: M
authors.lastname: Evert
mesh_terms: D000818:Animals; D022781:Hepatocytes / Q000166:cytology; D021621:Imaging, Three-Dimensional; D016381:Islets of Langerhans Transplantation; D008099:Liver / Q000098:blood supply* / Q000166:cytology*; D008833:Microcirculation; D008953:Models, Anatomic; D011169:Portal Vein; D051381:Rats; D011917:Rats, Inbred Lew; D013961:Thyroid Gland / Q000166:cytology* / Q000637:transplantation