Gene gun-delivered pGM-CSF adjuvant induces enhanced emigration of two dendritic cell subsets from the skin.
Two subsets of sheep afferent lymph dendritic cells (DC) are defined by the differential expression of CD172a and CD45RA. The majority (~70%) of CD172a(+) subset is CD45RA/CD11c(+)/CD207(+)/TLR4(+). The CD172a(-) DC are CD45RA(+)/CD207(-) and express low levels of CD11c and CD86. Real-time RT-PCR showed that CD172(+) DC produce IL-1beta and IL-10 and high levels of IL-18 but almost no IL-12p40; CD172a(-) DC express IL-12p40 but no IL-10 and low levels of IL-1beta and IL-18. Gene gun-delivered granulocyte-macrophage colony-stimulating factor (pGM-CSF) caused an early rise in the output of CD172a(+) DC, changes to DC phenotype and significant increases in the levels of expression cytokine transcripts. However, pGM-CSF did not affect any qualitative changes to cytokine expression, CD172a(+) DC remained IL-10(+)/IL-12p40(-) and the CD172(-) DC remained IL-10(-)/IL-12p40(+).
Matthews
K
K
Centre for Infectious Diseases, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, UK.
Bailey
S L
SL
Gossner
A G
AG
Watkins
C
C
Dalziel
R G
RG
Hopkins
J
J
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D019470:Biolistics; D002465:Cell Movement / Q000276:immunology*; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology; D005434:Flow Cytometry; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000008:administration & dosage*; D008196:Lymph / Q000166:cytology; D010641:Phenotype; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012756:Sheep; D012867:Skin / Q000166:cytology*
D000276:Adjuvants, Immunologic; D016207:Cytokines; D016178:Granulocyte-Macrophage Colony-Stimulating Factor