title: Long serial analysis of gene expression for transcriptome profiling during the initiation of ligninolytic enzymes production in Phanerochaete chrysosporium.
abstract: To analyze the transcriptome profile during the initiation of manganese peroxidase (MnP) and lignin peroxidase (LiP) production in Phanerochaete chrysosporium, we constructed long serial analysis of gene expression (LongSAGE) libraries. A total of 13,666 tags (the number of cumulative counted tags) that included 6,945 unique tags (the number of distinct tags) were isolated from the day-3 culture, which just started the enzymes production and was 24 h after veratryl alcohol addition and oxygen-purge into the culture (day-2 culture). A total of 12,402 tags that included 6,396 unique tags were isolated from the day-2 culture, in which the activity of enzymes is not detected. The comparison of the two libraries suggested that 38 genes showed significant (p < or = 0.01) fourfold or greater upregulation; this included the MnP gene (mnp2, mnp3) and LiP H8 gene. On the other hand, 43 genes showed significant (p < or = 0.01) fourfold or greater downregulation. This LongSAGE analysis found many new candidate genes regulating the enzymes production.
authors.affiliation: Department of Environmental Science, The University of Shiga Prefecture, 2500 Hassaka-cho, Hikone, Shiga, 522-8533, Japan.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Minami
authors.forename: Orie
authors.initials: O
authors.lastname: Kureha
authors.forename: Mari
authors.initials: M
authors.lastname: Mori
authors.forename: Hisatoshi
authors.initials: H
authors.lastname: Kamitsuji
authors.forename: Kazumi
authors.initials: K
authors.lastname: Suzuki
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Irie
mesh_terms: D020869:Gene Expression Profiling / Q000379:methods*; D015723:Gene Library; D005800:Genes, Fungal; D008031:Lignin / Q000378:metabolism; D010544:Peroxidases / Q000235:genetics* / Q000378:metabolism; D020075:Phanerochaete / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
chemical_list: D008031:Lignin; D010544:Peroxidases; C042858:lignin peroxidase; C051129:manganese peroxidase