Midgut protease activities in monophagous larvae of Apollo butterfly, Parnassius apollo ssp. frankenbergeri.
We assayed the relative activities of midgut proteolytic enzymes in individuals of the fourth (L(4)) and fifth (L(5)) instar of Apollo larvae, inhabiting Pieniny Mts (southern Poland). The comparisons between midgut tissue with glicocalyx (MT) and liquid midgut contents with peritrophic membrane (MC) were made. Optimal media pHs of the assayed proteolytic enzymes in P. apollo midgut samples were similar to those of other lepidopteran species. Endopeptidases, as well as carboxypeptidases, digested effectively in alkaline environment, while aminopeptidases were active in a broad pH range. Trypsin is probably the main endoprotease (correlation with caseinolytic activity in MC of L(5) larvae: r=0.606; p=0.004); however, its activity was low as compared with that in other leaf-eating Lepidoptera. This suggests a minor role of trypsin and chymotrypsin in protein digestion in Apollo larvae, probably due to limited availability of the leaf proteins. Instead, due to very high carboxypeptidase A activity in midgut tissue, the larvae obtain exogenous amino acids either directly or from oligopeptides and glycoproteins. High and significant positive correlations between the enzyme activity and glucosidase as well as galactosidase activities strongly support this opinion.
Nakonieczny
Mirosław
M
University of Silesia, Department of Animal Physiology and Ecotoxicology, Bankowa 9, 40-007 Katowice, Poland. nakon@us.edu.pl
Michalczyk
Katarzyna
K
Kedziorski
Andrzej
A
D000626:Aminopeptidases / Q000032:analysis; D000818:Animals; D002080:Butterflies / Q000201:enzymology / Q000254:growth & development*; D043422:Carboxypeptidases A / Q000032:analysis; D002918:Chymotrypsin / Q000032:analysis; D005696:Galactosidases / Q000032:analysis; D005959:Glucosidases / Q000032:analysis; D006026:Glycoside Hydrolases / Q000032:analysis*; D019476:Insect Proteins / Q000032:analysis; D007422:Intestines / Q000201:enzymology*; D007814:Larva / Q000201:enzymology; D010447:Peptide Hydrolases / Q000032:analysis*; D011044:Poland; D014357:Trypsin / Q000032:analysis
D019476:Insect Proteins; D005696:Galactosidases; D005959:Glucosidases; D006026:Glycoside Hydrolases; D010447:Peptide Hydrolases; D000626:Aminopeptidases; D043422:Carboxypeptidases A; D002918:Chymotrypsin; D014357:Trypsin