[Study on supercritical carbon dioxide fluid extraction process of lignans from Kadsura heteroclita (Roxb.) Craib].
OBJECTIVE
To optimize supercritical carbon dioxide fluid extraction process parameters of lignans from Kadsura heteroclita (Roxb.) Craib.


METHODS
The supercritical carbon dioxide fluid extraction was adopted to extract lignans. The contents of total lignans and gamma-schisandrin were determined to test the influence of pressure, temperature, grinding particle size and flow rate of CO2.


RESULTS
The extraction pressure and grinding particle size had significant effects on the content of total lignans and gamma-schisandrin. However, The extraction temperature and flow rate of CO2 had no significant effect on them.


CONCLUSIONS
The optimum process parameters are as fouons: pressure is 10 MPa, temperature is 45 degrees C, flow rate of CO2 is 30 L/h and herbs are granulated.
Li
Xiao-guang
XG
College of Pharmacy, Jinan University, Guangzhou 510632, China. morninglight202@163.com
Gao
Qin
Q
Weng
Wen
W
Luo
Huan-min
HM
D000704:Analysis of Variance; D002245:Carbon Dioxide; D002851:Chromatography, High Pressure Liquid; D025924:Chromatography, Supercritical Fluid / Q000379:methods; D034242:Cyclooctanes / Q000032:analysis / Q000302:isolation & purification; D032135:Kadsura / Q000737:chemistry*; D017705:Lignans / Q000032:analysis / Q000302:isolation & purification*; D010316:Particle Size; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011312:Pressure; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature
D034242:Cyclooctanes; D017705:Lignans; D002245:Carbon Dioxide