title: Establishment of cell lines derived from the rat suprachiasmatic nucleus.
abstract: Physiological and behavioral circadian rhythms in mammals are orchestrated by a central circadian clock located in the suprachiasmatic nucleus (SCN) of the hypothalamus. Photic input entrains the phase of the central clock, and many peripheral clocks are regulated by neural or hormonal output from the SCN. We established cell lines derived from the rat embryonic SCN to examine the molecular network of the central clock. An established cell line exhibited the stable circadian expression of clock genes. The circadian oscillation was abruptly phase-shifted by forskolin, and abolished by siBmal1. These results are compatible with in vivo studies of the SCN.
authors.affiliation: Research Group of Chronogenomics, Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194-8511, Japan.
authors.forename: Soshi
authors.initials: S
authors.lastname: Kawaguchi
authors.forename: Atsuki
authors.initials: A
authors.lastname: Shinozaki
authors.forename: Masuo
authors.initials: M
authors.lastname: Obinata
authors.forename: Kaoru
authors.initials: K
authors.lastname: Saigo
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Sakaki
authors.forename: Hajime
authors.initials: H
authors.lastname: Tei
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D001683:Biological Clocks / Q000235:genetics; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology; D017931:DNA Primers; D020869:Gene Expression Profiling; D007150:Immunohistochemistry; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013493:Suprachiasmatic Nucleus / Q000166:cytology* / Q000187:drug effects / Q000196:embryology / Q000378:metabolism
chemical_list: D017931:DNA Primers; D005576:Colforsin