Mapuera virus, a rubulavirus that inhibits interferon signalling in a wide variety of mammalian cells without degrading STATs.
Mapuera virus (MPRV) is a paramyxovirus that was originally isolated from bats, but its host range remains unknown. It was classified as a member of the genus Rubulavirus on the basis of structural and genetic features. Like other rubulaviruses it encodes a V protein (MPRV/V) that functions as an interferon (IFN) antagonist. Here we show that MPRV/V differs from the IFN antagonists of other rubulaviruses in that it does not induce the proteasomal degradation of STAT proteins, key factors in the IFN signalling cascade. Rather, MPRV/V prevents the nuclear translocation of STATs in response to IFN stimulation and inhibits the formation of the transcription factor complex ISGF3. We also show that MPRV/V blocks IFN signalling in cells from diverse mammalian species and discuss the IFN response as a barrier to cross-species infections.
Hagmaier
K
K
School of Biology, University of St Andrews, Fife KY16 9TS, UK.
Stock
N
N
School of Biology, University of St Andrews, Fife KY16 9TS, UK.
Precious
B
B
School of Biology, University of St Andrews, Fife KY16 9TS, UK.
Childs
K
K
Division of Basic Medical Sciences, St George's, University of London, London SW17 0RE, UK.
Wang
L-F
LF
CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, VIC 3220, Australia.
Goodbourn
S
S
Division of Basic Medical Sciences, St George's, University of London, London SW17 0RE, UK.
Randall
R E
RE
School of Biology, University of St Andrews, Fife KY16 9TS, UK.
D021581:Active Transport, Cell Nucleus; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D006801:Humans; D050832:Interferon-Stimulated Gene Factor 3 / Q000378:metabolism; D007372:Interferons / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D010766:Phosphorylation; D019213:Rubulavirus / Q000276:immunology*; D050791:STAT Transcription Factors / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology*
D050832:Interferon-Stimulated Gene Factor 3; D050791:STAT Transcription Factors; D014764:Viral Proteins; D007372:Interferons