[Myocardial reporter gene expression imaging].
Radionuclide gene expression imaging can visualize and measure transcription activity of target genes noninvasively by co-expressing a reporter gene, of which product can be traced with complementary radioactive reporter probes. While in the cardiac field it was at first aiming at monitoring angiogenic gene therapy, its application is directed to cell tracking in stem/progenitor cell therapy, due to disappointing results in the clinical trials and potential risks of transfer vectors. In this article, we give a general outline of cardiac radionuclide gene expression imaging using reporter genes, including its principle, various combinations of reporter gene/reporter probe, the current status of cardiac gene therapy, and its application for cell tracking.
Inubushi
Masayuki
M
Department of Molecular Imaging, Hokkaido University Graduate School of Medicine.
Yoshinaga
Keiichiro
K
Morita
Koichi
K
Tamaki
Nagara
N
D000818:Animals; D017690:Cell Transplantation; D015870:Gene Expression; D017930:Genes, Reporter; D015316:Genetic Therapy; D011877:Radionuclide Imaging; D051381:Rats