title: Development and characterization of an infectious cDNA clone of the virulent Bucyrus strain of Equine arteritis virus.
abstract: Strains of Equine arteritis virus (EAV) differ in the severity of the disease that they induce in horses. Infectious cDNA clones are potentially useful for identification of genetic determinants of EAV virulence; to date, two clones have been derived from a cell culture-adapted variant of the original (Bucyrus) isolate of EAV, and it has previously been shown that recombinant virus derived from one of these (rEAV030) is attenuated in horses. A complete cDNA copy of the genome of the virulent Bucyrus strain of EAV has now been assembled into a plasmid vector. In contrast to rEAV030, recombinant progeny virus derived from this clone caused severe disease in horses, characterized by pyrexia, oedema, leukopenia, high-titre viraemia and substantial nasal shedding of virus. The availability of infectious cDNA clones that produce recombinant viruses of different virulence to horses will facilitate characterization of the virulence determinants of EAV through reverse genetics.
authors.affiliation: Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
authors.forename: Udeni B R
authors.initials: UBR
authors.lastname: Balasuriya
authors.affiliation: Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Snijder
authors.affiliation: Department of Biology, University of Texas at San Antonio, TX 78249, USA.
authors.forename: Hans W
authors.initials: HW
authors.lastname: Heidner
authors.affiliation: Department of Veterinary Science, Gluck Equine Research Center, University of Kentucky, Lexington, KY 40546, USA.
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Jessika C
authors.initials: JC
authors.lastname: Zevenhoven-Dobbe
authors.affiliation: Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
authors.forename: Josh D
authors.initials: JD
authors.lastname: Boone
authors.affiliation: Department of Veterinary Science, Gluck Equine Research Center, University of Kentucky, Lexington, KY 40546, USA.
authors.forename: William H
authors.initials: WH
authors.lastname: McCollum
authors.affiliation: Department of Veterinary Science, Gluck Equine Research Center, University of Kentucky, Lexington, KY 40546, USA.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Timoney
authors.affiliation: Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
authors.forename: N James
authors.initials: NJ
authors.lastname: MacLachlan
mesh_terms: D000818:Animals; D018174:Arterivirus Infections / Q000503:physiopathology / Q000662:veterinary* / Q000821:virology; D018076:DNA, Complementary; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D007240:Equartevirus / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology*; D005822:Genetic Vectors; D016679:Genome, Viral; D006734:Horse Diseases / Q000503:physiopathology / Q000821:virology*; D006736:Horses; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D014766:Viremia; D017201:Virus Shedding
chemical_list: D018076:DNA, Complementary; D004279:DNA, Viral