Pleiotrophin promotes functional recovery after neural transplantation in rats.
Pleiotrophin promotes survival of dopaminergic neurons in vitro. To investigate whether pleiotrophin promotes survival of grafted dopaminergic neurons in vivo, donor cells from ventral mesencephalon were treated with pleiotrophin (100 ng/ml) during cell preparation and grafted into striatum of hemi-Parkinson model rats. Functional recovery in methamphetamine-induced rotations was improved, and more tyrosine hydroxylase-positive cells survived in the striatum in the pleiotrophin-treated group. Pleiotrophin addition to cells just before transplantation also resulted in better functional recovery; however, no caspase-3 activation was seen during cell preparation. Interestingly, the effect of pleiotrophin on the survival was additive to that of glial-cell line-derived neutropic factor. These results revealed that pleiotrophin had effects on donor cells in neural transplantation in vivo.
Hida
Hideki
H
Department of Neurophysiology and Brain Science, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan. hhida@med.nagoya-cu.ac.jp
Masuda
Tadashi
T
Sato
Toyohiro
T
Kim
Tae-Sun
TS
Misumi
Sachiyo
S
Nishino
Hitoo
H
D000818:Animals; D016380:Brain Tissue Transplantation; D002352:Carrier Proteins / Q000494:pharmacology*; D003342:Corpus Striatum / Q000166:cytology / Q000601:surgery; D016207:Cytokines / Q000494:pharmacology*; D004195:Disease Models, Animal; D004298:Dopamine / Q000502:physiology; D005260:Female; D016332:Fetal Tissue Transplantation; D006085:Graft Survival / Q000187:drug effects / Q000502:physiology; D008636:Mesencephalon / Q000166:cytology; D009414:Nerve Growth Factors / Q000494:pharmacology; D009474:Neurons / Q000637:transplantation*; D010300:Parkinson Disease / Q000473:pathology / Q000628:therapy*; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function / Q000187:drug effects* / Q000502:physiology
D002352:Carrier Proteins; D016207:Cytokines; D009414:Nerve Growth Factors; C066845:pleiotrophin; D004298:Dopamine