title: Remodeling of hepatic vascular changes after specific chemotherapy of schistosomal periportal fibrosis.
abstract: Hepatosplenic schistosomiasis was the first human disease in which the possibility of extensive long standing hepatic fibrosis being degraded and removed has been demonstrated. When such changes occurred, the main signs of portal hypertension (splenomegaly, esophageal varices) progressively disappeared, implying that a profound vascular remodeling was concomitantly occurring. Hepatic vascular alterations associated with advanced schistosomiasis have already been investigated. Obstruction of the intrahepatic portal vein branches, plus marked angiogenesis and compensatory hyperplasia and hypertrophy of the arterial tree are the main changes present. However, there are no data revealing how these vascular changes behave during the process of fibrosis regression. Here the mouse model of pipestem fibrosis was used in an investigation about these vascular alterations during the course of the infection, and also after treatment and cure of the disease. Animals representing the two polar hepatic forms of the infection were included: (1) "isolated granulomas" characterized by isolated periovular granulomas sparsely distributed throughout the hepatica parenchyma; and (2) 'pipestem fibrosis' with periovular granulomas and fibrosis being concentrated within portal spaces, before and after treatment, were studied by means of histological and vascular injection-corrosion techniques. Instances of widespread portal vein obstruction of several types were commonly found in the livers of the untreated animals. These obstructive lesions were soon repaired, and completely disappeared four months following specific treatment of schistosomiasis. Treatment was accomplished by the simultaneous administration of praziquantel and oxamniquine. The most impressive results were revealed by the technique of injection of colored masses into the portal system, followed by corrosion in strong acid. The vascular lesions of non-treated pipestem fibrosis were represented in the plastic casts by considerable diminution of the fine peripheral portal vein radicles, plus dilatation of periportal collaterals. Four months after treatment, this last picture appeared replaced by tufts of newly interwoven vessels formed along the main portal vein branches, disclosing a strong angiomatoid reparative change. Understanding about the cellular elements at play during fibro-vascular repairing changes of hepatic schistosomiasis represents a matter of considerable scientific and conceptual importance. At present time one may only speculate about the participation of some type of natural stem-cell capable of restoring the diseased liver back to normal once the cause of the disorder has been eliminated.
authors.affiliation: Laboratório de Patologia Experimental, Centro de Pesquisas Gonçalo Moniz-Fiocruz, Rua Valdemar Falcão 121, 40295-000 Salvador, BA, Brazil. zilton@cpqgm.fiocruz.br
authors.forename: Zilton A
authors.initials: ZA
authors.lastname: Andrade
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Baptista
authors.forename: Thaynã Souto
authors.initials: TS
authors.lastname: Santana
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000627:therapeutic use; D002908:Chronic Disease; D004195:Disease Models, Animal; D005260:Female; D006099:Granuloma / Q000473:pathology; D006801:Humans; D008102:Liver Circulation / Q000502:physiology*; D008103:Liver Cirrhosis / Q000469:parasitology / Q000473:pathology* / Q000503:physiopathology; D008109:Liver Diseases, Parasitic / Q000473:pathology* / Q000503:physiopathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010073:Oxamniquine / Q000627:therapeutic use; D011168:Portal System / Q000469:parasitology / Q000473:pathology* / Q000503:physiopathology; D011223:Praziquantel / Q000627:therapeutic use; D012555:Schistosomiasis mansoni / Q000150:complications* / Q000188:drug therapy / Q000473:pathology
chemical_list: D000871:Anthelmintics; D010073:Oxamniquine; D011223:Praziquantel