Contrasting effects of FLIPL overexpression in human T cells on activation-induced cell death and cytokine production.
There have been disparate findings about the role of FLIP in the survival of mouse T cells and human tumor cell lines. The role of cellular FLIP in human T cell activation and function needs to be clarified further. To study this role, we have overexpressed long transcript FLIP (FLIPL) in primary T cells, including self-antigen-reactive, melanoma-specific T cells. We found that FLIPL overexpression protects human T cells from activation-induced cell death and enhances their proliferative capacity but suppresses the ability of these cells to produce the proinflammatory cytokines IL-2 and IFN-gamma in response to CD3 or antigen-specific stimulation. The multiple effects of FLIPL indicate that this protein may influence T cell responses to antigenic stimulation.
Charo
Jehad
J
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA. j.charo@mdc-berlin.de
Robbins
Paul F
PF
D053446:CASP8 and FADD-Like Apoptosis Regulating Protein / Q000096:biosynthesis* / Q000235:genetics; D016923:Cell Death / Q000276:immunology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D016207:Cytokines / Q000096:biosynthesis*; D006801:Humans; D013601:T-Lymphocytes / Q000276:immunology*
D053446:CASP8 and FADD-Like Apoptosis Regulating Protein; D016207:Cytokines