title: Embedding of individual ferritin molecules in large, self-supporting silica nanofilms.
abstract: We report herein the fabrication of ferritin-embedded self-supporting silica nanofilms via a simple spin-coating process. Ferritin was employed as a template molecule, and solutions of ferritin and silica were spread on a polymer-coated silicon substrate, in this order. After dissolving the polymer underlayer by simply immersing ethanol, a centimeter-sized self-supporting nanofilm of ferritin/silica composite with a thickness of 15 nm was successfully transferred onto an alumina membrane without the film breaking. Ozone and hydrochloric acid solution treatment removed the template ferritin molecules from the composite film to produce corresponding transmembrane nanoholes. The reported method is very simple, and the fabrication of a protein-embedded self-supporting nanofilm enables the design of biomembrane-mimetic devices.
authors.affiliation: Topochemical Design Laboratory, and Innovative Nanopatterning Laboratory, RIKEN, Wako, Saitama 351-0198, Japan. sfuji@riken.jp
authors.forename: Shigenori
authors.initials: S
authors.lastname: Fujikawa
authors.forename: Emi
authors.initials: E
authors.lastname: Muto
authors.forename: Toyoki
authors.initials: T
authors.lastname: Kunitake
mesh_terms: D032701:Biomimetics; D002627:Chemistry, Physical / Q000379:methods; D000431:Ethanol / Q000737:chemistry; D005293:Ferritins / Q000737:chemistry*; D006851:Hydrochloric Acid / Q000737:chemistry; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D010126:Ozone / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D012825:Silicon / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D011108:Polymers; D000431:Ethanol; D010126:Ozone; D012822:Silicon Dioxide; D005293:Ferritins; D006851:Hydrochloric Acid; D012825:Silicon