Characterisation of porcine bone marrow progenitor cells identified by the anti-c-kit (CD117) monoclonal antibody 2B8/BM.
c-kit (CD117) plays an important role in the early stages of haematopoiesis. Previous studies of porcine haematopoietic stem cells have relied for their identification on the use of the c-kit ligand stem cell factor. Here, we describe a new mAb, 2B8/BM, that recognizes a 155-kDa protein expressed on a small subset (2-8%) of bone marrow haematopoietic cells. 2B8/BM(+) cells have a blast appearance, and are mostly negative for lineage-specific markers or express low levels of CD172a or SLA-II. In in vitro colony-forming unit assays these cells were able to give rise to erythroid and myeloid colonies. Altogether these data suggested that the 2B8/BM antigen might be the porcine orthologue of the human c-kit. This specificity was confirmed by the binding of mAb 2B8/BM to CHO cells transfected with a plasmid encoding the porcine c-kit ectodomain. This antibody can facilitate the isolation and enrichment of porcine stem cells to be used in procedures aimed to induce xenograft tolerance or to test their potential to repair damaged tissues and organs.
Pérez
C
C
Dpto. Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 28040, Madrid, Spain.
Moreno
S
S
Summerfield
A
A
Domenech
N
N
Alvarez
B
B
Correa
C
C
Alonso
F
F
Ezquerra
A
A
Domínguez
J
J
Revilla
C
C
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity; D001854:Bone Marrow Cells / Q000276:immunology*; D016466:CHO Cells; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D006224:Cricetinae; D003412:Cricetulus; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006825:Hybridomas / Q000378:metabolism; D007150:Immunohistochemistry; D016130:Immunophenotyping; D010641:Phenotype; D019009:Proto-Oncogene Proteins c-kit / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D013552:Swine; D014162:Transfection
C519884:2B8-BM monoclonal antibody; D000911:Antibodies, Monoclonal; D019009:Proto-Oncogene Proteins c-kit