[Experimental studies on a new bone tissue engineered scaffold biomaterials combined with cultured marrow stromal stem cells in vitro].
OBJECTIVE
To explore the biocompatibility of poly (lactic acid/glycolic acid/asparagic acid-copolyethylene glycol) biomaterials (PLGA-ASP-PEG) and biological behaviors of cultured marrow stroml stem cells (MSCs) combined with this new type of scaffold in tissue engineering.


METHODS
The PLGA-ASP-PEG tri-block copolymers were obtained through bulk ring-opening copolymerization method. MSCs were isolated from the bone marrow of 4-week-old New Zealand rabbits. The 3rd-generation MSCs were cultured combining with PLGA-ASP-PEG in vitro, while cells cultured in PLGA as control group. The cell adhesion rate and the adhesive power were examined by conventional precipitation method and micropipette aspiration technique respectively. The morphological features were studied by scanning electron microscope. The proliferation behavior of the cells was analyzed by MTT assay. The cell cycle, proliferation index, DNA index and apoptosis of the cells were detected by flow cytometry. The synthesis of protein and collagen were examined by Coomassie Brilliant Blue dyes and 3H-Proline incorporation test.


RESULTS
The MSCs adhered and grew well on the surface of the biomaterial PLGA-ASP-PEG. The powers of cell adhesion, proliferation and protein and collagen synthesis of the cells were all significantly higher than those of PLGA group (P< 0.05), but the apoptosis rate was significantly lower than that of PLGA group (P < 0.05). The DNA indexes showed the cells of both PLGA-ASP-PEG group and PLGA group were normal diploid cells.


CONCLUSIONS
PLGA-ASP-PEG showed good biocompatibility and the biological properties improved greatly compared with the PLGA scaffold materials. These results demonstrated that the promise of PLGA-ASP-PEG can be used as an ideal scaffold material for construction of tissue engineered bone to restore bone defects in bone tissue engineering.
Pan
Haitao
H
Department of Orthopedics, Affiliated Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan Hubei, 430022, P. R.China.
Zheng
Qixin
Q
Guo
Xiaodong
X
D000818:Animals; D001224:Aspartic Acid / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry; D001854:Bone Marrow Cells / Q000166:cytology*; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D019344:Lactic Acid / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D011817:Rabbits; D017154:Stromal Cells / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; D011100:Polyglycolic Acid; D001224:Aspartic Acid; D019344:Lactic Acid