Establishment of cell lines derived from the rat suprachiasmatic nucleus.
Physiological and behavioral circadian rhythms in mammals are orchestrated by a central circadian clock located in the suprachiasmatic nucleus (SCN) of the hypothalamus. Photic input entrains the phase of the central clock, and many peripheral clocks are regulated by neural or hormonal output from the SCN. We established cell lines derived from the rat embryonic SCN to examine the molecular network of the central clock. An established cell line exhibited the stable circadian expression of clock genes. The circadian oscillation was abruptly phase-shifted by forskolin, and abolished by siBmal1. These results are compatible with in vivo studies of the SCN.
Kawaguchi
Soshi
S
Research Group of Chronogenomics, Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194-8511, Japan.
Shinozaki
Atsuki
A
Obinata
Masuo
M
Saigo
Kaoru
K
Sakaki
Yoshiyuki
Y
Tei
Hajime
H
D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D001683:Biological Clocks / Q000235:genetics; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology; D017931:DNA Primers; D020869:Gene Expression Profiling; D007150:Immunohistochemistry; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013493:Suprachiasmatic Nucleus / Q000166:cytology* / Q000187:drug effects / Q000196:embryology / Q000378:metabolism
D017931:DNA Primers; D005576:Colforsin