title: Isolation and characterization of a cDNA encoding a CBF transcription factor from E. globulus.
abstract: The transcription factors CBF/DREB play an important role during low temperature, drought and high-salt stress in higher plants. In this work, we isolated one full-length CBF cDNA clone from the angiosperm Eucalyptus globulus. The derived peptide sequence reveals that it encodes a transcriptional activator that has all the characteristic motifs present in CBF proteins previously described in Arabidopsis and tomato. RT-PCR analysis shows that EgCBF1 is transiently induced in E. globulus seedlings that had been exposed to low temperature within the first 15 min. These results suggest that the isolated CBF gene participates in the cold responsive pathway of E. globulus.
authors.affiliation: Instituto Milenio MIFAB - Fundación Ciencia para la Vida, Zañartu 1482, Nuñoa, Santiago, Chile.
authors.forename: Maria C
authors.initials: MC
authors.lastname: Gamboa
authors.forename: Susana
authors.initials: S
authors.lastname: Rasmussen-Poblete
authors.forename: Pablo D T
authors.initials: PD
authors.lastname: Valenzuela
authors.forename: Erwin
authors.initials: E
authors.lastname: Krauskopf
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics; D023081:CCAAT-Binding Factor / Q000235:genetics*; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics*; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics*; D005052:Eucalyptus / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics / Q000302:isolation & purification; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D029681:Arabidopsis Proteins; D023081:CCAAT-Binding Factor; D017931:DNA Primers; D018076:DNA, Complementary; D018744:DNA, Plant; D010940:Plant Proteins; D018749:RNA, Plant