title: Black porgy (Acanthopagrus schlegeli) prolactin cDNA sequence: mRNA expression and blood physiological responses during freshwater acclimation.
abstract: To investigate the consequences of freshwater (FW) transfer, we studied the prolactin (PRL) cDNA sequence and its mRNA expression, and physiological responses in the black porgy (Acanthopagrus schlegeli). We cloned and characterized cDNA encoding its PRL from the pituitary gland. Black porgy PRL cDNA consists of 1492 bp and encodes a protein of 212 amino acids including 24 signal peptides. Reverse transcription-PCR showed the PRL mRNA expression in the pituitary gland. Expression of pituitary gland PRL mRNA was significantly higher during FW acclimation. Furthermore, we studied the stress responses and osmoregulatory abilities of black porgy in changing salinities. Plasma cortisol, glucose, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) significantly increased in the fish immediately after transfer to FW. We also identified significant changes in the fish in terms of plasma ions (Na(+), Cl(-), Ca(2+)) and osmolality during the acclimation period. These results suggests that PRL plays an important role in hormonal regulation in osmoregulatory organs, thereby improving the hyperosmoregulatory ability of black porgy in freshwater.
authors.affiliation: Department of Aquaculture, Pukyong National University, Busan, Republic of Korea.
authors.forename: Young Jin
authors.initials: YJ
authors.lastname: Chang
authors.forename: Byung Hwa
authors.initials: BH
authors.lastname: Min
authors.forename: Cheol Young
authors.initials: CY
authors.lastname: Choi
mesh_terms: D000064:Acclimatization; D000410:Alanine Transaminase / Q000097:blood; D000595:Amino Acid Sequence; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001483:Base Sequence; D001786:Blood Glucose / Q000032:analysis; D002118:Calcium / Q000097:blood; D002712:Chlorides / Q000097:blood; D018076:DNA, Complementary / Q000235:genetics*; D005618:Fresh Water; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006854:Hydrocortisone / Q000097:blood; D008969:Molecular Sequence Data; D009994:Osmolar Concentration; D010473:Perciformes / Q000097:blood* / Q000235:genetics*; D010902:Pituitary Gland / Q000378:metabolism; D011388:Prolactin / Q000097:blood / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012964:Sodium / Q000097:blood
chemical_list: D001786:Blood Glucose; D002712:Chlorides; D018076:DNA, Complementary; D012333:RNA, Messenger; D011388:Prolactin; D012964:Sodium; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D002118:Calcium; D006854:Hydrocortisone