Expression, crystallization and X-ray data collection from microcrystals of the extracellular domain of the human inhibitory receptor expressed on myeloid cells IREM-1.
IREM-1 is an inhibitory receptor involved in the functional regulation of myeloid cells. The expression, in vitro folding, purification, crystallization and X-ray data collection of the Ig-V like domain of IREM-1 are reported. X-ray data were collected from a microcrystal (300 x 10 x 10 microm) at 100 K and a diffraction pattern was obtained to 2.6 A resolution on microfocus beamline ID23-2 at the ESRF. The crystal belongs to space group P3(1)21, with unit-cell parameters a = b = 54.23, c = 72.02 A, alpha = gamma = 90, beta = 120 degrees. Assuming the presence of one molecule per asymmetric unit, V(M) (the Matthews coefficient) was calculated to be 1.96 A3 Da(-1) and the solvent content was estimated to be 37.27%. Determination of the IREM-1 structure will provide insights into its structural requirements for ligand discrimination and binding.
Dimasi
Nazzareno
N
Istituto Giannina Gaslini, Largo Gerolamo Gaslini 5, 16147 Genova, Italy. ndimasi@gmail.com
Flot
David
D
Dupeux
Florine
F
Márquez
José A
JA
D000954:Antigens, Surface / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D001483:Base Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D045604:Extracellular Fluid / Q000737:chemistry / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D022423:Myeloid Cells / Q000737:chemistry / Q000378:metabolism*; D017510:Protein Folding; D017434:Protein Structure, Tertiary
D000954:Antigens, Surface; C444139:CD300C protein, human; D007135:Immunoglobulin Variable Region; D008562:Membrane Glycoproteins