[Differentiation of infection with Mycobacterium tuberculosis by recombinant Mycobacterium tuberculosis 11000 protein].
OBJECTIVE
To study the differentiation effect of recombinant Mycobacterium tuberculosis 11000 protein on infection of Mycobacterium tuberculosis.


METHODS
Guinea pigs were immunized with different strains of mycobacterium, and then all guinea pigs were given intradermal injections with recombinant Mycobacterium tuberculosis 11000 protein and purified protein derivative of tuberculin (PPD) or purified protein derived from M. intracellulare (PPD-B). Skin reactions defined with two transverse diameters were read double-blinded after 24 and (or) 48 hours, and the means of the two transverse diameters were counted as the reaction diameters.


RESULTS
All guinea pigs immunized with different strains of Mycobacteria responded to PPD or PPD-B with positive skin reactions. The recombinant Mycobacterium tuberculosis 11000 protein elicited positive skin reactions in guinea pigs infected with live Mycobacterium tuberculosis, Mycobacterium bovis, Mycobacterium africanum and Mycobacterium kansasii, and the reaction diameters were (14.7 +/- 2.0) mm, (9.3 +/- 3.8) mm, (18.7 +/- 2.4) mm and (14.8 +/- 4.2) mm, respectively. But it failed to elicit positive skin reaction in guinea pigs immunized with killed Mycobacterium tuberculosis, live BCG and other MOTT (mycobacteria other than Mycobacterium tuberculosis).


CONCLUSIONS
Recombinant Mycobacterium tuberculosis 11000 protein can differentiate infection with live Mycobacterium tuberculosis from immunization with killed Mycobacterium tuberculosis, live BCG or other MOTT.
Xu
Miao
M
Department of Pulmonary, the First Affiliated Hospital, Chongqing University of Medical Science, Chongqing 400016, China.
Luo
Yong-ai
YA
Li
You-lun
YL
Chen
Bao-wen
BW
Shen
Xiao-bing
XB
Su
Cheng
C
Wang
Guo-zhi
GZ
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D003937:Diagnosis, Differential; D005260:Female; D006168:Guinea Pigs; D009164:Mycobacterium Infections / Q000145:classification / Q000175:diagnosis*; D009169:Mycobacterium tuberculosis; D011994:Recombinant Proteins / Q000235:genetics; D012882:Skin Tests; D013045:Species Specificity
D001426:Bacterial Proteins; D011994:Recombinant Proteins