Simultaneous use of multiple fluorescent reporter dyes for glucose sensing in aqueous solution.
The simultaneous use of several fluorescent reporter dyes in a multicomponent boronic acid-based glucose sensing system is reported. In one application, two dyes with widely different emission wavelengths are used to report changes in glucose concentration. A third glucose-insensitive dye was then added to act as a reference dye and provide for a ratiometric correction to the two reporter dye signals. The inclusion of such a reference dye reduces errors arising from sources such as fluctuations in lamp intensity and sample dilution.
Cordes
David B
DB
Department of Chemistry and Biochemistry, University of California, Santa Cruz, 1156 High Street, Santa Cruz, CA 95064, USA.
Miller
Aaron
A
Gamsey
Soya
S
Singaram
Bakthan
B
D005456:Fluorescent Dyes / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D012996:Solutions; D014867:Water / Q000737:chemistry
D005456:Fluorescent Dyes; D012996:Solutions; D014867:Water; D005947:Glucose