Activity of the notch-signalling pathway in circulating human chronic lymphocytic leukaemia cells.
Dysregulation of the Notch-pathway has been implicated in the pathogenesis of chronic lymphocytic leukaemia (B-CLL). We characterized the mRNA expression of Notch pathway elements in circulating normal B- and B-CLL cells, and compared expression profiles with clinical and prognostic data. Similar expression profiles were found in normal B-cells and B-CLL cells, however, most B-CLL samples showed lower Hairy/Enhancer of Split-1 expression than normal B-cells, which suggests that the pathway is not over-activated in B-CLL. The expression of Notch-pathway genes did not correlate with other prognostic factors of B-CLL. The importance of Notch-signalling in CLL cells in lymphatic tissue microenvironments remains to be determined.
Hajdu
M
M
I Department of Pathology and Experimental Cancer Research, Budapest, Hungary. melindahajdu@freemail.hu
Sebestyén
A
A
Barna
G
G
Reiniger
L
L
Jánosi
J
J
Sréter
L
L
Várkonyi
J
J
Demeter
J
J
Kopper
L
L
D051997:ADP-ribosyl Cyclase 1 / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D015153:Blotting, Western; D005434:Flow Cytometry; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D007143:Immunoglobulin Heavy Chains; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000378:metabolism*; D011379:Prognosis; D012333:RNA, Messenger / Q000032:analysis; D051880:Receptors, Notch / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000276:immunology*; D027041:Somatic Hypermutation, Immunoglobulin
D007143:Immunoglobulin Heavy Chains; D012333:RNA, Messenger; D051880:Receptors, Notch; D051997:ADP-ribosyl Cyclase 1