title: Development and validation of a lateral flow device for the detection of nicarbazin contamination in poultry feeds.
abstract: Concentrations of the coccidiostat nicarbazin as low as 2 mg/kg in feed can result in violative drug residues arising in poultry liver. A lateral flow device (LFD) was developed for the detection of contaminating concentrations of nicarbazin following solvent extraction of poultry feeds. Test results, as determined by both visual and instrumental measurement, are available within minutes. For 22 feed samples, nicarbazin-free and fortified at 2 mg/kg, the % relative inhibition ranged from 0 to 45% and from 53 to 85%, respectively. Nicarbazin contamination at the critical concentration (2 mg/kg) can be determined in all cases providing the sampling is representative. A wide range of feed samples taken at a mill that incorporated nicarbazin into poultry feed were analyzed. Data generated for these samples by both the LFDs and a mass spectrometric method were compared, and a significant correlation was achieved.
authors.affiliation: Institute of Agri-Food and Land Use, Queen's University, David Keir Building, Stranmillis Road, Belfast BT9 5AG, UK. katrina.campbell@qub.ac.uk
authors.forename: Katrina
authors.initials: K
authors.lastname: Campbell
authors.forename: Terence
authors.initials: T
authors.lastname: Fodey
authors.forename: Jonathan
authors.initials: J
authors.lastname: Flint
authors.forename: Christopher
authors.initials: C
authors.lastname: Danks
authors.forename: Martin
authors.initials: M
authors.lastname: Danaher
authors.forename: Michael
authors.initials: M
authors.lastname: O'Keeffe
authors.forename: D Glenn
authors.initials: DG
authors.lastname: Kennedy
authors.forename: Christopher
authors.initials: C
authors.lastname: Elliott
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D003049:Coccidiostats / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D009528:Nicarbazin / Q000032:analysis*; D011200:Poultry; D015203:Reproducibility of Results
chemical_list: D003049:Coccidiostats; D009528:Nicarbazin