title: Structural analysis of mucoidan, an acidic extracellular polysaccharide produced by a pristane-assimilating marine bacterium, Rhodococcus erythropolis PR4.
abstract: Rhodococcus erythropolis PR4 is a marine bacterium that can degrade various alkanes including pristane, a C(19) branched alkane. This strain produces a large quantity of extracellular polysaccharides (EPS), which are assumed to play an important role in the hydrocarbon tolerance of R. erythropolis PR4. The strain produced an acidic EPS, mucoidan, together with a fatty acid-containing EPS, PR4 FACEPS. The chemical structure of the mucoidan was determined using (1)H and (13)C NMR spectroscopy and by conducting 2D DQF-COSY, TOCSY, HMQC, HMBC, and NOESY experiments. The mucoidan was shown to consist of a pentasaccharide repeating unit with the following structure: [structure: see text].
authors.affiliation: Laboratory of Molecular Microbiology, Department of Applied Biological Science, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa 252-8510, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Urai
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yoshizaki
authors.forename: Hirosi
authors.initials: H
authors.lastname: Anzai
authors.forename: Jun
authors.initials: J
authors.lastname: Ogihara
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Iwabuchi
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Harayama
authors.forename: Michio
authors.initials: M
authors.lastname: Sunairi
authors.forename: Mutsuyasu
authors.initials: M
authors.lastname: Nakajima
mesh_terms: D002240:Carbohydrate Sequence; D005110:Extracellular Space / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008745:Methylation; D009005:Monosaccharides / Q000032:analysis / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D012240:Rhodococcus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012623:Seawater / Q000382:microbiology*; D013729:Terpenes / Q000378:metabolism*
chemical_list: D009005:Monosaccharides; D011135:Polysaccharides, Bacterial; D013729:Terpenes; C520410:mucoidan, Rhodococcus erythropolis; C009042:pristane