title: Low prevalence of Chlamydia psittaci in ocular adnexal lymphomas from Cuban patients.
abstract: Most ocular adnexal lymphomas (OAL) are extranodal marginal zone B-cell lymphomas (EMZL) of mucosa-associated lymphoid tissue (MALT)-type. Chronic antigen stimulation has been suggested to have a pathogenetic role in EMZL and Chlamydia psittaci chronic infection has been recently associated with the development of OAL in a series of patients from Italy. To assess this association, an evaluation of the presence of C. psittaci was made in a different OAL population. DNA samples were obtained from formalin-fixed, paraffin-embedded sections samples of 26 patients with OAL, 20 non-OAL and 20 benign ocular lesions, diagnosed and treated between 1998 and 2003 at National Institute of Oncology in Havana, Cuba. All samples were histologically reviewed by an expert pathologist. Fluorescence in situ hybrization (FISH) analysis of translocations involving MALT1 was performed. The presence of bacterial DNA was assessed with a multiplex touchdown enzyme time release polymerase chain reaction. DNA sequencing was performed to confirm suspicious bands. Seventy-three percent of the OAL cases were EMZL and 81% were in stage IE. FISH analysis was performed in 13 OAL cases and none of them evidenced MALT1 translocations. DNA of C. psittaci was detected in 11% of the 46 lymphomas: two orbital EMZL and three non-OAL. All 20 benign ocular lesions were negative for C. psittaci. The low prevalence of C. psittaci in OAL suggests geographical differences in the etiology of this entity. International studies are needed to clarify the role of C. psittaci in OALs.
authors.affiliation: National Institute of Oncology and Radiobiology, La Habana, Cuba.
authors.forename: Elías
authors.initials: E
authors.lastname: Gracia
authors.forename: Patrizia
authors.initials: P
authors.lastname: Froesch
authors.forename: Luca
authors.initials: L
authors.lastname: Mazzucchelli
authors.forename: Vittoria
authors.initials: V
authors.lastname: Martin
authors.forename: Delvys
authors.initials: D
authors.lastname: Rodríguez-Abreu
authors.forename: Julio
authors.initials: J
authors.lastname: Jiménez
authors.forename: María
authors.initials: M
authors.lastname: Melgares
authors.forename: Dania
authors.initials: D
authors.lastname: Santos
authors.forename: Virginia
authors.initials: V
authors.lastname: Capó
authors.forename: Franco
authors.initials: F
authors.lastname: Cavalli
authors.forename: Emanuele
authors.initials: E
authors.lastname: Zucca
authors.forename: Francesco
authors.initials: F
authors.lastname: Bertoni
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D020169:Caspases / Q000235:genetics; D002691:Chlamydophila psittaci / Q000235:genetics / Q000302:isolation & purification*; D003462:Cuba / Q000453:epidemiology; D004269:DNA, Bacterial / Q000302:isolation & purification; D005134:Eye Neoplasms / Q000150:complications / Q000235:genetics / Q000382:microbiology*; D005260:Female; D006801:Humans; D016393:Lymphoma, B-Cell / Q000150:complications / Q000235:genetics / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D000074884:Mucosa-Associated Lymphoid Tissue Lymphoma Translocation 1 Protein; D009363:Neoplasm Proteins / Q000235:genetics; D015995:Prevalence; D009956:Psittacosis / Q000150:complications* / Q000453:epidemiology; D012189:Retrospective Studies
chemical_list: D004269:DNA, Bacterial; D009363:Neoplasm Proteins; D020169:Caspases; C400109:MALT1 protein, human; D000074884:Mucosa-Associated Lymphoid Tissue Lymphoma Translocation 1 Protein