[rgpB genetic polymorphism of Porphyromonas gingivalis in subgingival plaque].
OBJECTIVE
To analyse the genetic polymorphism of Arg-gingipainB (rgpB), a virulent factors of Porphyromonas gingivalis (P.gingivalis) and discuss the role of the different genotypes in the genesis and progress of chronic periodontitis.


METHODS
A total of 104 subgingival plaque samples were included in this study. The extracted DNA was amplified with the primers designed to obtain the gene encoding the catalytic domain of rgpB (rgpB-cd), P.gingivalis was typed into four genotypes by restriction fragment length polymorphism (RFLP).


RESULTS
In lesion site, the detection rate of type IV was the highest (52.78%), which was higher than those of type I and III (P < 0.05 or P < 0.01). While in non-lesion site, The detection rate of type II was the highest (75.86%), which was higher than those of other types (P < 0.01).


CONCLUSIONS
The polymorphism of rgpB-cd gene may influence the virulence of P.gingivalis. P.gingivalis type IV may be well related to periodontitis, while type II may be a indigenous flora.
Li
Chen
C
Department of Periodontology, School of Stomatology, China Medical University, Shengyang 110002, China.
Pan
Ya-ping
YP
Lin
Li
L
D018829:Adhesins, Bacterial / Q000235:genetics*; D000328:Adult; D000368:Aged; D055113:Chronic Periodontitis / Q000382:microbiology*; D003546:Cysteine Endopeptidases / Q000235:genetics*; D003773:Dental Plaque / Q000382:microbiology; D005260:Female; D000080867:Gingipain Cysteine Endopeptidases; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D016966:Porphyromonas gingivalis / Q000235:genetics* / Q000472:pathogenicity
D018829:Adhesins, Bacterial; D000080867:Gingipain Cysteine Endopeptidases; D003546:Cysteine Endopeptidases