Electrochemically addressable functionalization and parallel readout of a DNA biosensor array.
We introduce an electrochemical method for the directed in situ assembly of various specific oligonucleotide capture probes onto different sensing elements of a multielectrode array in the aqueous environment of a flow cell. Surface plasmon microscopy is utilized for the on-line recording of the various functionalization steps. Hybridization reactions between targets from solution to the different surface-bound complementary probes are monitored by surface plasmon field-enhanced fluorescence microscopy using targets that are either labeled with organic dyes or with semiconducting quantum dots for color-multiplexing. This study provides a new approach for the fabrication of (small) DNA arrays and the recording and quantitative evaluation of parallel hybridization reactions.
Niu
Lifang
L
Institute of Materials Research & Engineering, 3 Research Link, Singapore.
Knoll
Wolfgang
W
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D004563:Electrochemistry; D004566:Electrodes; D008856:Microscopy, Fluorescence / Q000295:instrumentation; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods*; D015345:Oligonucleotide Probes / Q000737:chemistry; D045663:Quantum Dots; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods; D013997:Time Factors; D014867:Water / Q000737:chemistry
D015345:Oligonucleotide Probes; D014867:Water