Separation and determination of three water-soluble compounds in Salvia miltiorrhiza Bunge and two related traditional medicinal preparations by flow injection-capillary electrophoresis.
A sensitive and reproducible method was developed for the separation and determination of three water-soluble components-protocatechuic aldehyde (PAH), beta-(3,4-dihydroxyphenyl) lactic acid (DSS) and protocatechuic acid (PA) in medicine plant Salvia miltiorrhiza Bunge and two related traditional medicinal preparations using flow injection-capillary electrophoresis system. This analysis was carried out by using an unmodified fused-silica capillary (28.4 cm x 75 microm i.d.x 375 microm o.d., 25 cm effective separation length) and direct ultraviolet detection at 214 nm, 7.0 kV applied voltage. With boric acid (200 mM) adjusted to pH 7.8 as a background electrolyte. The separation was achieved in 9 min. The sample throughput rate could reach up to 15 h(-1). Calibration curves showed good linearity with correlation coefficients (r) more than 0.9986. The repeatability (defined as R.S.D.) were 0.20%, 0.46%, 0.47% with migration time evaluation and 0.62%, 3.66%, 1.50% with peak area evaluation for PAH, DSS and PA, respectively. The limits of detection (S/N=3) were 0.36 microg/mL, 0.84 microg/mL, and 0.73 microg/mL for PAH, DSS, and PA, respectively. The mean recoveries of PAH, DSS and PA were 103.2%, 98.1% and 100.5%, respectively. This method has been applied successfully to monitor these three components in Salvia miltiorrhiza Bunge and its two traditional medicinal preparations.
Liu
Xiumei
X
Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
Zhang
Jingshu
J
Chen
Xingguo
X
D002138:Calibration; D019075:Electrophoresis, Capillary / Q000379:methods*; D008516:Medicine, Chinese Traditional; D015203:Reproducibility of Results; D027544:Salvia / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012995:Solubility; D014867:Water
D014867:Water