title: Tanshinone IIA from Salvia miltiorrhiza induces heme oxygenase-1 expression and inhibits lipopolysaccharide-induced nitric oxide expression in RAW 264.7 cells.
abstract: Tanshinone IIA exerts anti-inflammatory effects and influences electron transfer reaction in mitochondria. In the present study, we demonstrated that tanshinone IIA increased intracellular production of reactive oxygen species (ROS), which in turn induces heme oxygenase-1 (HO-1) expression in RAW 264.7 macrophages. Tanshinone IIA inhibited COX-2 and iNOS expression in lipopolysaccharide-activated RAW 264.7 macrophages. Inhibition of HO-1 or scavenging of CO significantly reversed the inhibition of LPS-stimulated nitrite accumulation by tanshinone IIA, suggesting a novel role of HO-1 in the anti-inflammatory effect of tanshinone IIA.
authors.affiliation: Department of Internal Medicine, Taipei Municipal Wan-Fang Hospital, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan.
authors.forename: Tso-Hsiao
authors.initials: TH
authors.lastname: Chen
authors.forename: Yu-Tern
authors.initials: YT
authors.lastname: Hsu
authors.forename: Cheng-Hsien
authors.initials: CH
authors.lastname: Chen
authors.forename: Shu-Hwei
authors.initials: SH
authors.lastname: Kao
authors.forename: Horng-Mo
authors.initials: HM
authors.lastname: Lee
mesh_terms: D045784:Abietanes; D000818:Animals; D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000096:biosynthesis; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000201:enzymology; D008565:Membrane Proteins / Q000096:biosynthesis*; D051379:Mice; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors; D010616:Phenanthrenes / Q000494:pharmacology*; D027561:Salvia miltiorrhiza / Q000737:chemistry; D015398:Signal Transduction
chemical_list: D045784:Abietanes; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D010616:Phenanthrenes; C021751:tanshinone; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse; D051546:Cyclooxygenase 2; D048049:Extracellular Signal-Regulated MAP Kinases