title: C-kit positive interstitial cells of Cajal network in primary obstructive megaureter.
abstract: OBJECTIVE
  Recent reports have shown an altered density of interstitial cells of Cajal (ICCs) and peripherin immunoreactive nerve fibres in uretopelvic junction obstruction. The aim was to investigate ICCs immunoexpression and ureteral innervation in primary obstructive megaureter (POM).
  
  
  METHODS
  Eight specimens of POM were obtained during tailoring. Restricted segments of ureters were divided from dilated segments. C-kit and peripherin immunohistochemistry were performed.
  
  
  RESULTS
  A normal distribution of ICCs was observed in both the circular and longitudinal muscle layers of the dilated segments. Marked muscle hypoplasia and sparse or no ICCs occurred in the longitudinal muscle layer of the restricted ureteral segments. A normal distribution of peripherin positive fibres was present in both the dilated and restricted segments.
  
  
  CONCLUSIONS
  Our data confirm defective muscularization in restricted aperistaltic POM. The lack of ICCs in the longitudinal muscular layer is probably due to the absence of c-kit positive muscle embryological precursors. No alteration in the peripherin immunoreactive nerve fibres network was observed in either the dilated or the restricted ureteral segments.
authors.affiliation: Unit of Pediatric Surgery, Department of Medical and Surgical Pediatric Sciences, University of Messina, Messina, Italy. arenasal@inwind.it
authors.forename: F
authors.initials: F
authors.lastname: Arena
authors.forename: P A
authors.initials: PA
authors.lastname: Nicòtina
authors.forename: S
authors.initials: S
authors.lastname: Arena
authors.forename: C
authors.initials: C
authors.lastname: Romeo
authors.forename: B
authors.initials: B
authors.lastname: Zuccarello
authors.forename: G
authors.initials: G
authors.lastname: Romeo
mesh_terms: D020541:Coiled Bodies / Q000378:metabolism*; D005260:Female; D006801:Humans; D007223:Infant; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D008297:Male; D009130:Muscle, Smooth / Q000378:metabolism / Q000473:pathology*; D009412:Nerve Fibers / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000378:metabolism; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism*; D014517:Ureteral Obstruction / Q000378:metabolism* / Q000473:pathology*
chemical_list: D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; D019009:Proto-Oncogene Proteins c-kit