title: Analysis of underivatized gentamicin by capillary electrophoresis with UV detection.
abstract: A selective and rapid capillary zone electrophoresis method for determination of the multicomponent aminoglycoside antibiotic gentamicin is described. Baseline separation of gentamicin C1, C1a, C2, C2a and C2b components was achieved with a background electrolyte containing 0.35 mM cetyl trimethylammonium bromide, 3% methanol and 90 mM sodium pyrophosphate (pH 7.4) and detected directly with UV detection without derivatization. Quantitative analysis was performed and illustrated the potential use of capillary electrophoresis for the identification and quantitation of gentamicin components, but the application of this method is limited to a gentamicin concentration range of 2-6 mg/ml.
authors.affiliation: Centro de Química Farmacéutica, Calle 200 y 21, Atabey, Playa, P.O. Box 16042, Habana, Cuba.
authors.forename: Harold
authors.initials: H
authors.lastname: Curiel
authors.forename: W
authors.initials: W
authors.lastname: Vanderaerden
authors.forename: Herman
authors.initials: H
authors.lastname: Velez
authors.forename: Jos
authors.initials: J
authors.lastname: Hoogmartens
authors.forename: Ann
authors.initials: A
authors.lastname: Van Schepdael
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D011756:Diphosphates / Q000737:chemistry; D004573:Electrolytes / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D005839:Gentamicins / Q000032:analysis* / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D000432:Methanol / Q000737:chemistry; D015394:Molecular Structure; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D002593:Cetrimonium Compounds; D011756:Diphosphates; D004573:Electrolytes; D005839:Gentamicins; C003319:sodium pyrophosphate; D000432:Methanol; D000077286:Cetrimonium