Examining Ca2+ extrusion of Na+/Ca2+-K+ exchangers.
Na+/Ca2+-K+ exchangers (NCKX) are plasma membrane transporters that are thought to mainly mediate Ca2+ extrusion (along with K+) at the expense of the Na+ electrochemical gradient. However, because they are bidirectional, most assays have relied on measuring their activity in the reverse (Ca2+ import) mode. Herein we describe a method to control intracellular ionic conditions, and examine the forward (Ca2+ extrusion) mode of exchange of NCKX2.
Altimimi
Haider F
HF
Department of Physiology and Biophysics, Faculty of Medicine, University of Calgary, 3330 Hospital Dr., N.W. Calgary, Alberta, T2N 4N1, Canada.
Schnetkamp
Paul P M
PP
D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D004563:Electrochemistry; D006801:Humans; D019831:Sodium-Calcium Exchanger / Q000378:metabolism*
D019831:Sodium-Calcium Exchanger; D002118:Calcium