Enzymatic production of galactooligosaccharides by beta-galactosidase from Bifidobacterium longum BCRC 15708.
The production of galactooligosaccharides (GOSs) by transgalactosylation using beta-galactosidase from Bifidobacterium longum BCRC 15708 was studied. Other than lactose, galactose, and glucose, two types of GOSs, tri- and tetrasaccharides, were formed after beta-galactosidase action on 40% lactose. Trisaccharides were the major type of GOS formed. Generally, an increase of the initial lactose concentration in the reaction mixture resulted in a higher GOS production. A maximum yield of 32.5% (w/w) GOSs could be achieved from 40% lactose solution at 45 degrees C, pH 6.8, when the lactose conversion was 59.4%. The corresponding productivity of GOSs was 13.0 g/(L.h). Transgalactosylation activity of beta-galactosidase from a test organism showed a relatively lower sensitivity toward glucose and galactose than that from other organisms. The addition of 5% or 10% glucose or galactose to the reaction mixture did not significantly (p>0.05) reduce the transgalactosylation reaction of beta-galactosidase.
Hsu
C A
CA
Graduate Institute of Food Science & Technology, National Taiwan University, Taipei, Taiwan.
Lee
S L
SL
Chou
C C
CC
D001644:Bifidobacterium / Q000201:enzymology*; D005690:Galactose / Q000378:metabolism* / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D007785:Lactose / Q000032:analysis / Q000494:pharmacology; D009844:Oligosaccharides / Q000096:biosynthesis*; D001616:beta-Galactosidase / Q000378:metabolism*
D009844:Oligosaccharides; D001616:beta-Galactosidase; D005947:Glucose; D007785:Lactose; D005690:Galactose