title: Human cord blood CD133+ cells immunoselected by a clinical-grade apparatus differentiate in vitro into endothelial- and cardiomyocyte-like cells.
abstract: BACKGROUND
  Recent findings on human hematopoietic stem cell (HSC) properties suggest a possible therapeutic role of human umbilical cord blood (UCB) HSC-based cellular therapies in the treatment of myocardial infarction.
  
  
  METHODS
  Nine UCB units were subjected to sequential red cell removal, freezing, and postthawing CD133+ HSC immunoselection by a clinical-grade, CE-approved, magnetic apparatus and microbead-coated anti-CD133 monoclonal antibody. Selected UCB CD133+ cells were cultured in vitro in medium supporting either endothelial or cardiomyocytic differentiation in parallel experiments.
  
  
  RESULTS
  Immunoselection allowed recovery of 79 percent of initial CD133+ cells with a CD133+ cell purity of 81 percent, on average. Parallel cultures showed the appearance of endothelial markers (VE-cadherin, CD146, and KDR and bright expression of CD105), morphofunctional features of endothelium in endothelial-supporting cultures, of cardiac muscle proteins (troponin I and myosin ventricular heavy chain alpha/beta; MYHC) and specific gene expression (GATA4, NKX2.5, troponin I, and MYHC) in cardiomyocyte-oriented cultures.
  
  
  CONCLUSIONS
  The appearance of both endothelial- and cardiomyocyte-like cells from parallel cultures of frozen-thawed-immunoselected UCB CD133+ cells by a clinical-grade method and previously reported data on lack of major signs of rejection of these cells in immunocompetent rats subjected to experimental liver damage suggest a possible role of these allogeneic HSCs in cell therapies designed for regenerative treatments of ischemic diseases of human myocardium.
authors.affiliation: Department of Gynecology and Obstetrics, Catholic University Medical School, Rome, Italy.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Bonanno
authors.forename: Andrea
authors.initials: A
authors.lastname: Mariotti
authors.forename: Annabella
authors.initials: A
authors.lastname: Procoli
authors.forename: Maria
authors.initials: M
authors.lastname: Corallo
authors.forename: Sergio
authors.initials: S
authors.lastname: Rutella
authors.forename: Gloria
authors.initials: G
authors.lastname: Pessina
authors.forename: Giovanni
authors.initials: G
authors.lastname: Scambia
authors.forename: Salvatore
authors.initials: S
authors.lastname: Mancuso
authors.forename: Luca
authors.initials: L
authors.lastname: Pierelli
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism*; D002454:Cell Differentiation; D019070:Cell Lineage; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism / Q000637:transplantation*; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D006023:Glycoproteins / Q000276:immunology / Q000378:metabolism*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D009203:Myocardial Infarction / Q000628:therapy*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism / Q000637:transplantation*; D010455:Peptides / Q000276:immunology / Q000378:metabolism*; D011247:Pregnancy
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; C000606025:Prom1 protein, rat