Molecular epidemiological analysis of Japanese encephalitis virus in China.
Sixty-two new Japanese encephalitis virus (JEV) isolates were obtained from mosquitoes, biting midges, human cerebrospinal fluid and human blood samples in China during 2002-2005. The E and prM genes were sequenced and phylogenetic analyses were performed with 38 JEV other isolates from China and 36 JEV strains from other countries. Phylogenetic trees based on the E and prM gene sequences were similar. The results indicate that: (i) recent JEV isolates from China are divided into two genotypes, genotype 1 and genotype 3; (ii) recent JEV isolates from China are grouped into the same clusters within genotypes 1 and 3; and (iii) genotype 1 JEV strains have been isolated in China since 1979, whilst genotype 3 JEV strains were isolated before the 1970s. The results suggest that genotype 1 JEV was introduced to China around 1979 and that JEV strains belonging to genotypes 1 and 3 circulate in China.
Wang
Huan Yu
HY
State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 100 YingXinJie, XuanWuQu, Beijing 100052, People's Republic of China.
Takasaki
Tomohiko
T
Department of Virology 1, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
Fu
Shi Hong
SH
State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 100 YingXinJie, XuanWuQu, Beijing 100052, People's Republic of China.
Sun
Xiao Hong
XH
State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 100 YingXinJie, XuanWuQu, Beijing 100052, People's Republic of China.
Zhang
Hai Lin
HL
Department of Arbovirus and Chlamydia, Yunnan Institute of Endemic Diseases Control and Prevention, 33 Wenhua Rd, Dali City 67100, Yunnan Province, People's Republic of China.
Wang
Zhao Xiao
ZX
Department of Virology, Guizhou Province Center for Disease Control and Prevention, 73 BaGeYanLu, GuiYang City 550004, Guizhou Province, People's Republic of China.
Hao
Zong Yu
ZY
Department of Virology, Henan Province Center for Disease Control and Prevention, 47 WeiWuLu, Zhengzhou City 450003, Henan Province, People's Republic of China.
Zhang
Jia Ke
JK
Department of Virology, Sichuan Province Center for Disease Control and Prevention, 40 HuaiShuJie, Chendu City 610031, SiChuan Province, People's Republic of China.
Tang
Qing
Q
State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 100 YingXinJie, XuanWuQu, Beijing 100052, People's Republic of China.
Kotaki
Akira
A
Department of Virology 1, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
Tajima
Shigeru
S
Department of Virology 1, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
Liang
Xiao Feng
XF
Chinese Center for Disease Control and Prevention, 29 NanWeiLu, XuanWuQu, Beijing 100050, People's Republic of China.
Yang
Wei Zhong
WZ
Chinese Center for Disease Control and Prevention, 29 NanWeiLu, XuanWuQu, Beijing 100050, People's Republic of China.
Kurane
Ichiro
I
Department of Virology 1, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
Liang
Guo Dong
GD
State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 100 YingXinJie, XuanWuQu, Beijing 100052, People's Republic of China.
D000818:Animals; D001769:Blood / Q000821:virology; D002519:Ceratopogonidae / Q000821:virology; D002555:Cerebrospinal Fluid / Q000821:virology; D002681:China / Q000453:epidemiology; D009033:Culicidae / Q000821:virology; D004664:Encephalitis Virus, Japanese / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D004672:Encephalitis, Japanese / Q000453:epidemiology* / Q000821:virology*; D005814:Genes, Viral; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014759:Viral Envelope Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics
D008562:Membrane Glycoproteins; D012367:RNA, Viral; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C080628:glycoprotein E, Japanese encephalitis virus