Effect of lysozyme on plasma membranes of liver and heart cells: lectin binding assay.
Lectin binding assay was applied to polyacrylamide slices to study the effects of lysozyme on carbohydrate profile of plasma membranes from liver and heart tissues. The investigations were carried out on polyacrylamide slices immobilized with wheat germ agglutinin or Concanavalin A. It was shown that lysozyme changes the carbohydrate profile of plasma membranes of liver and heart cells.
Mikaelyan
M V
MV
Institute of Biochemistry, Physical Chemistry of Proteins, National Academy of Sciences, P.Sevak str. 5/1, Yerevan 375014, Armenia.
Poghosyan
G G
GG
Stepanyan
I E
IE
Gasparyan
V K
VK
D000180:Acrylic Resins / Q000737:chemistry; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D019411:Clinical Laboratory Techniques; D003208:Concanavalin A / Q000737:chemistry / Q000378:metabolism; D006321:Heart / Q000187:drug effects*; D022781:Hepatocytes / Q000187:drug effects* / Q000648:ultrastructure; D037102:Lectins / Q000378:metabolism*; D009113:Muramidase / Q000494:pharmacology*; D009206:Myocardium / Q000166:cytology / Q000648:ultrastructure; D011485:Protein Binding; D051381:Rats; D014020:Tissue Extracts / Q000737:chemistry; D014909:Wheat Germ Agglutinins / Q000737:chemistry
D000180:Acrylic Resins; D002241:Carbohydrates; D037102:Lectins; D014020:Tissue Extracts; D014909:Wheat Germ Agglutinins; C016680:polyacrylamide gels; D003208:Concanavalin A; D009113:Muramidase