Multilayer assembly of positively charged polyelectrolyte and negatively charged glucose oxidase on a 3D Nafion network for detecting glucose.
In this paper, a novel amperometric glucose biosensor was constructed by alternative self-assembly of positively charged poly(diallydimethylammonium chloride) (PDDA) and negatively charged glucose oxidase (GOx) onto a 3D Nafion network via electrostatic adsorption. The amount of Nafion in the electrode and the number of the (PDDA/GOx)(n) multilayers were optimized to develop a sensitive and selective glucose biosensor. Under optimal conditions, the glucose biosensor with (PDDA/GOx)(5) multilayers exhibited remarkable electrocatalytic activity, capable of detecting glucose with enhanced sensitivity of 9.55 microA/mM cm(2) and a commendably low detection limit of 20 microM (S/N=3). A linear response range of 0.05-7 mM (a linear correlation coefficient of 0.9984, n=20) was achieved. In addition, the glucose biosensor demonstrated superior selectivity towards glucose over some interferents, such as ascorbic acid (AA) and uric acid (UA), at an optimized detection potential of 0.6 V versus Ag/AgCl reference.
Chen
Xiaojun
X
School of Mechanical & Aerospace Engineering, Nanyang Technological University, Nanyang Avenue, Singapore 639798, Singapore. chenxj@pmail.ntu.edu.sg
Yan
Xingbin
X
Khor
Khiam Aik
KA
Tay
Beng Kang
BK
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D005465:Fluorocarbon Polymers / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry*; D011095:Polyethylenes / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*
D005465:Fluorocarbon Polymers; D011095:Polyethylenes; D000644:Quaternary Ammonium Compounds; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride; C040402:perfluorosulfonic acid; D005949:Glucose Oxidase; D005947:Glucose