title: Early-phase endotoxic shock-induced myocardial injury increases iNOS and selectin expression in macaque primate.
abstract: BACKGROUND
  Our previous study has established a macaque model with early-phase endotoxic shock. The present study further investigated myocardial and blood vessel injury in Macaques by examining the subsequent expression of ACP, selectins, iNOS, and cTnI in response to LPS treatment.
  
  
  METHODS
  In an experiment with anaesthetised, instrumental macaques, eleven animals were randomised into: an En group (n=6), receiving a dose of 2.8 mg kg(-1) lipopolysaccharides (LPS) by i.v.; and a Co group (n=5), injected with normal saline of 1 ml kg(-1). Cytochemistry of acid phosphatase (ACPase) in heart was performed by electron microscope at 120 min following endotoxin injection. Three immunochemical stains, namely, L-selectin, P-selectin and iNOS protein in heart, were studied. In addition, cardiac troponin I (cTnI), L-selectin and P-selectin in plasma were detected.
  
  
  RESULTS
  In the early phase of endotoxic shock, LPS caused myocardial lysosome damage. The data of immunochemical staining showed the thrombus formation in vessels and the increase of iNOS, L-Selectin and P-Selectin expression in heart, but LPS challenge did not change L-selectin, P-selectin and cTnI in plasma.
  
  
  CONCLUSIONS
  The increase of iNOS, L-selectin and P-selectin protein expression following endotoxin administration may have caused vessel injury and myocardial damage in macaques.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, 6 Tongwei Road, Nanjing, Jiangsu 210095, PR China. yinguoq@jlonline.com
authors.forename: Guo Qing
authors.initials: GQ
authors.lastname: Yin
authors.forename: Kai He
authors.initials: KH
authors.lastname: Du
authors.forename: Fei Rong
authors.initials: FR
authors.lastname: Gu
authors.forename: Zhi Xun
authors.initials: ZX
authors.lastname: Fang
authors.forename: Jia Qi
authors.initials: JQ
authors.lastname: Tang
authors.forename: Bei
authors.initials: B
authors.lastname: Zhong
authors.forename: Xiao Yun
authors.initials: XY
authors.lastname: Zhu
authors.forename: Ying Wei
authors.initials: YW
authors.lastname: Wu
authors.forename: Cheng Ping
authors.initials: CP
authors.lastname: Lu
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004926:Escherichia coli; D005260:Female; D019041:L-Selectin / Q000378:metabolism*; D008070:Lipopolysaccharides; D008251:Macaca; D008297:Male; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D019007:P-Selectin / Q000378:metabolism*; D012772:Shock, Septic / Q000150:complications* / Q000378:metabolism / Q000473:pathology; D019210:Troponin I / Q000097:blood
chemical_list: D008070:Lipopolysaccharides; D019007:P-Selectin; D019210:Troponin I; D019041:L-Selectin; D052247:Nitric Oxide Synthase Type II