Independent evolution of human immunodeficiency virus type 1 env V1/V2 and V4/V5 hypervariable regions during chronic infection.
Using DNA heteroduplex tracking assays, we characterized human immunodeficiency virus type 1 env V4/V5 genetic populations in multiple blood plasma samples collected over an average of 7 months from 24 chronically infected human subjects. We observed complex and dynamic V4/V5 genetic populations in most subjects. Comparisons of V4/V5 and V1/V2 population changes over the course of the study showed that major shifts in genetic populations frequently occurred in one region but not the other, and these observations were independently confirmed in one subject by single-genome sequencing. These results suggest that the V1/V2 and V4/V5 regions of env often evolve independently during chronic infection.
Harrington
Patrick R
PR
University of North Carolina at Chapel Hill, 22-062 Lineberger Cancer Center, CB#7295, Chapel Hill, NC 27599-7295, USA.
Nelson
Julie A E
JA
Kitrinos
Kathryn M
KM
Swanstrom
Ronald
R
D019143:Evolution, Molecular; D015699:HIV Envelope Protein gp120 / Q000235:genetics*; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000235:genetics*; D020180:Heteroduplex Analysis; D006801:Humans; D008969:Molecular Sequence Data; D010949:Plasma / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA
D015699:HIV Envelope Protein gp120; D012367:RNA, Viral