Microbial degradation of cyanobacterial cyclic peptides.
Bacterial strain B-9 possesses hydrolytic enzymes capable of degrading microcystins (MCs) and nodularin that are toxic cyclic peptides produced by cyanobacteria. In the present study, the degradation activities of the cell extract of B-9 against non-toxic cyanobacterial cyclic peptides other than the MCs and nodularin were investigated, and the degradation products were analyzed by liquid chromatography/ion trap tandem mass spectrometry (LC/ITMS). It was confirmed that B-9 could also degrade these cyanobacterial cyclic peptides by hydrolysis of their peptide bonds. These results indicated that this bacterium possesses a very unique hydrolytic activity that can degrade structurally different cyclic peptides and that this may be effective for the detoxification of hazardous cyclic peptides.
Kato
Hajime
H
Graduate School of Environmental and Human Sciences, Meijo University, 150, Yagotoyama, Tempaku-ku, Nagoya 468-8503, Japan. q0691501@ccmailg.meijo-u.ac.jp
Imanishi
Susumu Y
SY
Tsuji
Kiyomi
K
Harada
Ken-ichi
K
D001673:Biodegradation, Environmental; D000458:Cyanobacteria / Q000378:metabolism*; D010456:Peptides, Cyclic / Q000378:metabolism*; D042301:Sphingomonadaceae / Q000737:chemistry / Q000378:metabolism*
D010456:Peptides, Cyclic