title: Functional integrin subunits regulating cell-matrix interactions in the intervertebral disc.
abstract: Cellular interactions with the extracellular matrix are key factors regulating cell survival, differentiation, and response to environmental stimuli in cartilagenous tissues. Much is known about the extracellular matrix proteins in the intervertebral disc (IVD) and their variations with region, age, or degenerative state of the tissue. In contrast, little is known of the integrin cell surface receptors that directly bind to and interact with these matrix proteins in the IVD. In almost all tissues, these integrin-mediated cell-matrix interactions are important for transducing environmental cues arising from mechanical stimuli, matrix degradation fragments, and cytokines into intracellular signals. In this study, cells from the nucleus pulposus and anulus fibrosus regions of porcine IVDs were analyzed via flow cytometry to quantify integrin expression levels upon isolation and after monolayer culture. Assays of cell attachment to collagens, fibronectin, and laminin were performed after functional blocking of select integrin subunits to evaluate the role of specific integrins in cell attachment. In situ distribution and co-localization of integrins and laminin were also characterized. Results identify integrin receptors critical for IVD cell interactions with collagens (alpha1beta1) and fibronectin (alpha5beta1). Additionally, dramatic differences in cell-laminin interactions were observed between cells of the nucleus and anulus regions, including differences in alpha6 integrin expression, cell adhesion to laminin, and in situ pericellular environments. These findings suggest laminin-cell interactions may be important and unique to the nucleus pulposus region of the IVD. The results of this study provide new information on functional cell-matrix interactions in tissues of the IVD.
authors.affiliation: Department of Biomedical Engineering, Duke University, Durham, NC 27708-0281, USA.
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Gilchrist
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: William J
authors.initials: WJ
authors.lastname: Richardson
authors.forename: Richard F
authors.initials: RF
authors.lastname: Loeser
authors.forename: Lori A
authors.initials: LA
authors.lastname: Setton
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology*; D048429:Cell Size; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D005434:Flow Cytometry; D005782:Gels; D006706:Homeostasis / Q000502:physiology; D007150:Immunohistochemistry; D016023:Integrins / Q000378:metabolism*; D007403:Intervertebral Disc / Q000166:cytology* / Q000378:metabolism*; D007797:Laminin / Q000378:metabolism; D021122:Protein Subunits / Q000378:metabolism; D013552:Swine
chemical_list: D005353:Fibronectins; D005782:Gels; D016023:Integrins; D007797:Laminin; D021122:Protein Subunits; D003094:Collagen