[Haplotype and heteroplasmy in the HV II region of mtDNA in human blood detected by PCR-DGGE].
OBJECTIVE
To establish a simple and effective technique for detecting haplotype and heteroplasmy of mtDNA, and investigate their frequencies in Chinese Han population.


METHODS
The fragments from 29-290 nt of mtDNA HV II from peripheral leukocytes of 200 unrelated Wuhan Han individuals were analyzed by using PCR-DGGE technique.


RESULTS
Seventeen haplotypes were found in the range of 29-290 nt, and the haplotype diversity (HD) was 0.8844. The heterogeneity was observed from 4 individuals, and its frequency was 2%.


CONCLUSIONS
PCR-DGGE is a simple, sensitive and effective technique in analyzing polymorphism and heteroplasmy of mtDNA, and can be used in forensic practice.
Zhai
Xian-dun
XD
Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. sanmao506@163.com
Huang
Dai-xin
DX
Yin
Hui
H
Yang
Qin-en
QE
D044466:Asian People / Q000235:genetics; D002681:China / Q000208:ethnology; D004272:DNA, Mitochondrial / Q000097:blood / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D018740:Genetic Heterogeneity; D005828:Genetics, Population; D006239:Haplotypes; D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA
D004272:DNA, Mitochondrial