Halomonas alkaliphila sp. nov., a novel halotolerant alkaliphilic bacterium isolated from a salt pool in Campania (Italy).
A halotolerant and alkaliphilic Gram-negative bacterium, strain 18bAG(T), that grows aerobically at the optimum temperature of 37 degrees C, and at pH 7.5-10 (optimum 9.0), was isolated from a salt pool located in Montefredane in Campania Region (South of Italy). The isolate tolerated high concentration of NaCl up to 20%. Strain 18bAG(T) accumulated osmolytes and polyhydroxybutyrate, produced exopolysaccharide and possessed alpha-glucosidase activity. The predominant respiratory quinones were ubiquinones, Q8 and Q6(6H); phosphoethanolamine, phosphatidylglycerol and diphosphatidylglycerol were the predominant polar lipids. Major fatty acids were C16 : 1, C16 : 0, and C18 : 0. On the basis of 16S rRNA gene sequence similarity, 18bAG(T) was shown to belong to Halomonas genus. Analysis of 16S rRNA gene revealed a high similarity of strain 18bAG(T) to Halomonas venusta (DSM 4743(T)) and Halomonas hydrothermalis (DSM 15725(T)). Level of DNA-DNA relatedness between strain 18bAG(T) and the most related species Halomonas venusta and Halomonas hydrothermalis was 56.0% and 41.2%, respectively. The G+C content (mol%) of DNA was 53.0. The RiboPrinting patterns of Halomonas venusta and 18AG(T) showed a pattern similarity of 0.50. On the basis of genomic information and phenotypic characteristics strain 18bAG(T) represents a new species, for which the name Halomonas alkaliphila sp. nov. is proposed. The type strain is 18bAG(T) (=DSM 16354T =ATCC BAA-953T).
Romano
Ida
I
Istituto di Chimica Biomolecolare, Comprensorio ex Olivetti, Pozzouli, Na, Italy.
Lama
Licia
L
Nicolaus
Barbara
B
Poli
Annarita
A
Gambacorta
Agata
A
Giordano
Assunta
A
D000332:Aerobiosis; D000468:Alkalies / Q000737:chemistry; D001482:Base Composition; D001483:Base Sequence; D050260:Carbohydrate Metabolism; D004275:DNA, Ribosomal / Q000032:analysis; D020632:Halomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D006885:Hydroxybutyrates / Q000378:metabolism; D007558:Italy; D008969:Molecular Sequence Data; D010802:Phylogeny; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012492:Salts / Q000633:toxicity; D014871:Water Microbiology
D000468:Alkalies; D004275:DNA, Ribosomal; D006885:Hydroxybutyrates; D011135:Polysaccharides, Bacterial; D012336:RNA, Ribosomal, 16S; D012492:Salts