title: Comparison of the Digene Hybrid Capture System Cytomegalovirus (CMV) DNA (version 2.0), Roche CMV UL54 analyte-specific reagent, and QIAGEN RealArt CMV LightCycler PCR reagent tests using AcroMetrix OptiQuant CMV DNA quantification panels and specimens from allogeneic-stem-cell transplant recipients.
abstract: The Digene Hybrid Capture system cytomegalovirus (CMV) DNA (version 2.0), Roche CMV UL54 analyte-specific reagent, and QIAGEN RealArt CMV LightCycler PCR reagent tests were compared using whole-virus standards and plasma specimens collected from allogeneic-stem-cell transplant recipients. PCR assays showed better speed, sensitivity, and specificity.
authors.affiliation: Duke University Medical Center, Durham, NC 27710, USA. kim.hanson@duke.edu
authors.forename: Kimberly E
authors.initials: KE
authors.lastname: Hanson
authors.forename: L Barth
authors.initials: LB
authors.lastname: Reller
authors.forename: Joanne
authors.initials: J
authors.lastname: Kurtzberg
authors.forename: Mitchell
authors.initials: M
authors.lastname: Horwitz
authors.forename: Gwynn
authors.initials: G
authors.lastname: Long
authors.forename: Barbara D
authors.initials: BD
authors.lastname: Alexander
mesh_terms: D000328:Adult; D002648:Child; D002675:Child, Preschool; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000821:virology; D004279:DNA, Viral / Q000032:analysis / Q000097:blood / Q000302:isolation & purification; D004259:DNA-Directed DNA Polymerase / Q000032:analysis / Q000235:genetics; D006801:Humans; D007202:Indicators and Reagents; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D033581:Stem Cell Transplantation / Q000009:adverse effects*; D014184:Transplantation, Homologous / Q000009:adverse effects*; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics
chemical_list: D004279:DNA, Viral; D007202:Indicators and Reagents; D011933:Reagent Kits, Diagnostic; C109367:UL54 protein, Human herpesvirus 5; D014764:Viral Proteins; D004259:DNA-Directed DNA Polymerase