Coating electrospun collagen and gelatin fibers with perlecan domain I for increased growth factor binding.
Electrospun natural polymer membranes were fabricated from collagen or gelatin coated with a bioactive recombinant fragment of perlecan, a natural heparan sulfate proteoglycan. The electrospinning process allowed the facile processing of a three-dimensional, porous fibril (2-6 microm in diameter) matrix suitable for tissue engineering. Laser scanning confocal microscopy revealed that osteoblast-like MG63 cells infiltrated the depth of the electrospun membrane evenly without visible apoptosis. Tissue engineering scaffolds ideally mimic the extracellular matrix; therefore, the electrospun membrane must contain both structural and functional matrix features. Fibers were coated, after processing, with perlecan domain I (PlnDI) to improve binding of basic fibroblast growth factor (FGF-2), which binds to native heparan sulfate chains on PlnDI. PlnDI-coated electrospun collagen fibers were ten times more effective than heparin-BSA collagen fibers at binding FGF-2. Because FGF-2 modulates cell growth, differentiation, migration and survival, the ability to effectively bind FGF-2 to an electrospun matrix is a key improvement in creating a successful tissue engineering scaffold.
Casper
Cheryl L
CL
Department of Materials Science and Engineering, University of Delaware, Newark, Delaware 19716, USA.
Yang
Weidong
W
Farach-Carson
Mary C
MC
Rabolt
John F
JF
D002460:Cell Line; D003094:Collagen / Q000737:chemistry*; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D019812:Heparan Sulfate Proteoglycans / Q000737:chemistry*; D006801:Humans; D008567:Membranes, Artificial; D010316:Particle Size; D016062:Porosity; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D013499:Surface Properties; D013997:Time Factors
D019812:Heparan Sulfate Proteoglycans; D008567:Membranes, Artificial; D016222:Fibroblast Growth Factor 2; C071927:perlecan; D005780:Gelatin; D003094:Collagen