title: The involvement of protein kinase A in the immune response of Galleria mellonella larvae to bacteria.
abstract: The role of protein kinase A (PKA) in the humoral immune response of the greater wax moth Galleria mellonella larvae to live gram-positive bacteria Micrococcus lysodeikticus and gram-negative bacteria Escherichia coli was investigated. The immune challenge of larvae with both kinds of bacteria caused an increase in fat body PKA activity depending on the injected bacteria. Gram-positive M. lysodeikticus was a much better inducer of the enzyme activity than gram-negative E. coli. The PKA activity was increased about 2.5-fold and 1.5-fold, after M. lysodeikticus and E. coli injection, respectively. The in vivo inhibition of the enzyme activity by a cell permeable selective PKA inhibitor, Rp-8-Br-cAMPS, was correlated with considerable changes of fat body lysozyme content and hemolymph antimicrobial activity in bacteria-challenged insects. The kinetics of changes were different and dependent on the bacteria used for the immune challenge of G. mellonella larvae.
authors.affiliation: Department of Invertebrate Immunology, Institute of Biology, Maria Curie-Skłodowska University, Lublin, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Cytryńska
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Zdybicka-Barabas
authors.forename: Teresa
authors.initials: T
authors.lastname: Jakubowicz
mesh_terms: D000273:Adipose Tissue / Q000201:enzymology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000276:immunology*; D000917:Antibody Formation; D001419:Bacteria / Q000276:immunology; D017868:Cyclic AMP-Dependent Protein Kinases / Q000276:immunology* / Q000378:metabolism; D006458:Hemolymph / Q000276:immunology; D007814:Larva / Q000276:immunology* / Q000382:microbiology; D009036:Moths / Q000254:growth & development / Q000276:immunology* / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D017868:Cyclic AMP-Dependent Protein Kinases