title: A comprehensive analysis of allelic methylation status of CpG islands on human chromosome 11q: comparison with chromosome 21q.
abstract: It was generally believed that autosomal CpG islands (CGIs) escape methylation. However, our comprehensive analysis of allelic methylation status of 149 CGIs on human chromosome 21q revealed that a sizable fraction of them are methylated on both alleles even in normal blood cells. Here, we performed a similar analysis of 656 CGIs on chromosome 11q, which is gene-rich in contrast with 21q. The results indicate that 11q contains less methylated CGIs, especially those with tandem repeats and those in the coding or 3'-untranslated regions (UTRs), than 21q. Thus, methylation status of CGIs may substantially differ from one chromosome to another.
authors.affiliation: Department of Information and Systems Engineering, Faculty of Engineering, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Yamada
authors.forename: Tomoyo
authors.initials: T
authors.lastname: Shirakawa
authors.forename: Todd D
authors.initials: TD
authors.lastname: Taylor
authors.forename: Kohji
authors.initials: K
authors.lastname: Okamura
authors.forename: Hidenobu
authors.initials: H
authors.lastname: Soejima
authors.forename: Michiko
authors.initials: M
authors.lastname: Uchiyama
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Iwasaka
authors.forename: Tsunehiro
authors.initials: T
authors.lastname: Mukai
authors.forename: Ken-Ichiro
authors.initials: K
authors.lastname: Muramoto
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Sakaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Ito
mesh_terms: D001483:Base Sequence; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics*; D018899:CpG Islands / Q000235:genetics*; D019175:DNA Methylation; D017931:DNA Primers; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers