A new optical and nuclear dual-labeled imaging agent targeting interleukin 11 receptor alpha-chain.
Optical imaging has great potential for studying molecular recognitions both in vivo and in vitro, yet nuclear imaging is the most effective clinical molecular imaging modality. The combination of optical and nuclear imaging modalities may provide complementary information for improving diagnosis and management of diseases. In this study we developed an optical and nuclear dual-labeled imaging agent, 111In-DTPA-Bz-NH-SA-K(IR-783-S-Ph-CO)-c(CGRRAGGSC)NH2, called DLIA-IL11Ralpha. 111In-DTPA-Bz-NH-SA is the radiotracer moiety; a near-infrared dye IR-783-S-Ph-COOH serves as the fluorescent emitter; and the cyclic peptide c(CGRRAGGSC), which is known to target interleukin 11 receptor alpha-chain (IL-11Ralpha), delivers the desired imaging agent to its target. Experiments revealed that the cyclic peptide c(CGRRAGGSC) continued to possess the targeting capability to IL-11Ralpha after the conjugation of the optical and nuclear tracers. Furthermore, the presence of the metal isotope chelator did not cause quenching of fluorescence emission. The cross validation and direct comparison of optical and nuclear imaging of a tumor was achieved using a single injection, and the preliminary results show the conjugate has tumor targeting capabilities in vivo.
Wang
Wei
W
Department of Radiology, Division of Molecular Imaging, Baylor College of Medicine, Houston, Texas 77030, USA. ww1@bcm.edu
Ke
Shi
S
Kwon
Sunkuk
S
Yallampalli
Sasidhar
S
Cameron
Arlin G
AG
Adams
Kristen E
KE
Mawad
Michel E
ME
Sevick-Muraca
Eva M
EM
D000818:Animals; D001943:Breast Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D006801:Humans; D007205:Indium Radioisotopes / Q000493:pharmacokinetics; D008027:Light; D051379:Mice; D010446:Peptide Fragments / Q000493:pharmacokinetics; D053702:Receptors, Interleukin-11 / Q000378:metabolism*; D015899:Tomography, Emission-Computed, Single-Photon; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
D005456:Fluorescent Dyes; D007205:Indium Radioisotopes; D010446:Peptide Fragments; D053702:Receptors, Interleukin-11