Promoting potential of a Jamaica quassia extract in a rat medium-term hepatocarcinogenesis bioassay.
Jamaica quassia extract (JQE), a natural bittering agent, was investigated for hepatocarcinogenesis-promoting potential using a medium-term liver bioassay system. F344 male rats were given a single intraperitoneal injection of diethylnitrosamine (200mg/kg body weight) and then starting 2 weeks later, received JQE in the diet at concentrations of 500, 5000 or 30,000 ppm for 6 weeks. Animals for tumor promotion (+) and (-) controls were fed 500 ppm sodium phenobarbital (PB) and basal diet, respectively during the promotion phase in this model. All animals were subjected to two-thirds partial hepatectomy at week 3 and killed at week 8. As with the PB-promoted case, both numbers and areas of glutathione S-transferase placental form-positive liver cell foci were significantly increased by JQE at 30,000 ppm, with non-significant increases evident at 5000 ppm. The results thus indicate that JQE at high dose has promoting potential for rat hepatocarcinogenesis.
Woo
Gye-Hyeong
GH
Division of Pathology, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
Shibutani
Makoto
M
Inoue
Kaoru
K
Fujimoto
Hitoshi
H
Takahashi
Miwa
M
Lee
Kyoung-Youl
KY
Hirose
Masao
M
D000818:Animals; D002273:Carcinogens / Q000008:administration & dosage / Q000633:toxicity*; D004032:Diet; D004052:Diethylnitrosamine / Q000008:administration & dosage / Q000633:toxicity; D005982:Glutathione Transferase / Q000378:metabolism; D006498:Hepatectomy; D022781:Hepatocytes / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D007274:Injections, Intraperitoneal; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced* / Q000201:enzymology / Q000473:pathology; D008297:Male; D032270:Picrasma / Q000737:chemistry*; D010936:Plant Extracts / Q000008:administration & dosage / Q000633:toxicity*; D011230:Precancerous Conditions / Q000139:chemically induced* / Q000201:enzymology / Q000473:pathology; D032271:Quassia / Q000737:chemistry*; D051381:Rats; D011916:Rats, Inbred F344
D002273:Carcinogens; D010936:Plant Extracts; D004052:Diethylnitrosamine; D005982:Glutathione Transferase