title: Human endothelial progenitor cell attachment to polysaccharide-based hydrogels: a pre-requisite for vascular tissue engineering.
abstract: A hydrogel was prepared from polysaccharides (pullulan/dextran/fucoidan) and evaluated as a novel biomaterial for Endothelial Progenitor Cell (EPC) culture. Using a cross-linking process with sodium trimetaphosphate in aqueous solution, homogeneous, transparent and easy to handle gels were obtained with a water content higher than 90%. Circular scaffolds (6 mm diameter and 2 mm thickness discs) were used for cell culture. Different types of EPCs were used: CD34+ derived ECs from cord blood and two sorts of CD133+ derived ECs from human bone marrow, old (30 days) and young (4 days) cells. EPCs were characterised as endothelial cells by immunofluorescent stainings for CD31 and Dil-Ac-LDL. CD133+ derived ECs from bone marrow were characterized by RT-PCR for CD31, VE-cadherin and KDR. HSVECs (Human Saphenous Vein Endothelial Cells) were used as control cells. We evaluated whether different kinds of EPCs could adhere on this novel hydrogel 4 h and 24 h after seeding, by a colorimetric quantitative test. EPCs adhered to hydrogels in serum- free conditions with values being over than 80% for young CD133+ cells at 4 h and 24 h. This pullulan-based hydrogel could constitute a suitable support for vascular cell adhesion as a pre-requisite for vascular tissue engineering.
authors.affiliation: Inserm, U577, Bordeaux, F-33076 France. noelie.thebaud@bordeaux.inserm.fr
authors.forename: Noélie-Brunehilde
authors.initials: NB
authors.lastname: Thébaud
authors.forename: Dorothée
authors.initials: D
authors.lastname: Pierron
authors.forename: Reine
authors.initials: R
authors.lastname: Bareille
authors.forename: Catherine
authors.initials: C
authors.lastname: Le Visage
authors.forename: Didier
authors.initials: D
authors.lastname: Letourneur
authors.forename: Laurence
authors.initials: L
authors.lastname: Bordenave
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D001807:Blood Vessel Prosthesis; D001808:Blood Vessels / Q000166:cytology* / Q000254:growth & development*; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D008422:Materials Testing; D040542:Mechanotransduction, Cellular / Q000502:physiology; D010316:Particle Size; D011134:Polysaccharides / Q000737:chemistry*; D033081:Shear Strength; D013234:Stem Cells / Q000166:cytology / Q000502:physiology; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D011134:Polysaccharides