title: Dextransucrase production using cashew apple juice as substrate: effect of phosphate and yeast extract addition.
abstract: Cashew apples are considered agriculture excess in the Brazilian Northeast because cashew trees are cultivated primarily with the aim of cashew nut production. In this work, the use of cashew apple juice as a substrate for Leuconostoc mesenteroides cultivation was investigated. The effect of yeast extract and phosphate addition was evaluated using factorial planning tools. Both phosphate and yeast extract addition were significant factors for biomass growth, but had no significant effect on maximum enzyme activity. The enzyme activities found in cashew apple juice assays were at least 3.5 times higher than the activity found in the synthetic medium. Assays with pH control (pH = 6.5) were also carried out. The pH-controlled fermentation enhanced biomass growth, but decreased the enzyme activity. Crude enzyme free of cells produced using cashew apple juice was stable for 16 h at 30 degrees C at a pH of 5.0.
authors.affiliation: Departamento de Tecnologia de Alimentos, Universidade Federal do Ceara, Bloco 858--Campus do Pici, Fortaleza, CE, Brazil.
authors.forename: Clarice M A
authors.initials: CM
authors.lastname: Chagas
authors.forename: Talita L
authors.initials: TL
authors.lastname: Honorato
authors.forename: Gustavo A S
authors.initials: GA
authors.lastname: Pinto
authors.forename: Geraldo A
authors.initials: GA
authors.lastname: Maia
authors.forename: Sueli
authors.initials: S
authors.lastname: Rodrigues
mesh_terms: D001628:Beverages; D018533:Biomass; D001709:Biotechnology; D003470:Culture Media; D005285:Fermentation; D005964:Glucosyltransferases / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007934:Leuconostoc / Q000201:enzymology / Q000254:growth & development; D027845:Malus; D010710:Phosphates; D015003:Yeasts
chemical_list: D003470:Culture Media; D010710:Phosphates; D005964:Glucosyltransferases; C020025:dextransucrase