title: Influence of extracellular matrix coatings on implant stability and osseointegration: an animal study.
abstract: Aim of the present study was to test the hypothesis that the application of components of the extracellular matrix such as glycosaminoglycans used as implant surface coatings in combination with collagen, with and without growth factor, can lead to enhanced ossification and thus improve implant stability compared with collagen coatings alone. Twenty miniature pigs received 120 experimental titanium implants in the mandible. Three types of surface coatings were created: (1) collagen type I (coll), (2) collagen type I/chondroitin sulphate (coll/CS), (3) collagen type I/chondroitin sulphate/BMP-4 (coll/CS/BMP). Periimplant bone formation was assessed within a defined recess along the length axis of the implant. Bone-implant contact (BIC) and bone volume density (BVD) were determined, using both histomorphometry and synchrotron radiation micro computed tomography (SRmicroCT). To measure implant stability, resonance frequency analysis was applied after implantation and 1, 3, 7, and 22 weeks after placement. BIC was highest for coll/CS coated implants, followed by coll, p = 0.082. Histomorphometric BVD did not significantly change for any coating. SRmicroCT analysis showed an increased BVD for collagen coated implants, compared with the other two surface coatings. Implant stability showed a decrease for all coatings up to the third week. At 22 weeks, all coatings showed an increase in stability without reaching their initial level. Highest stability was reached for coll coated implants, p = 0.051. It was concluded that collagen and coll/CS implant coatings have advantageous characteristics for peri-implant bone formation, compared with the further integration of BMP-4.
authors.affiliation: Faculty of Medicine, Department of Maxillofacial Surgery, University of Technology Dresden, Fetscherstr. 74, D-01307 Dresden, Germany. stadlinger@gmx.de
authors.forename: Bernd
authors.initials: B
authors.lastname: Stadlinger
authors.forename: Eckart
authors.initials: E
authors.lastname: Pilling
authors.forename: Matthias
authors.initials: M
authors.lastname: Huhle
authors.forename: Ronald
authors.initials: R
authors.lastname: Mai
authors.forename: Susanne
authors.initials: S
authors.lastname: Bierbaum
authors.forename: Ricardo
authors.initials: R
authors.lastname: Bernhardt
authors.forename: Dieter
authors.initials: D
authors.lastname: Scharnweber
authors.forename: Eberhard
authors.initials: E
authors.lastname: Kuhlisch
authors.forename: Ute
authors.initials: U
authors.lastname: Hempel
authors.forename: Uwe
authors.initials: U
authors.lastname: Eckelt
mesh_terms: D000818:Animals; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins / Q000737:chemistry / Q000378:metabolism; D001842:Bone and Bones / Q000502:physiology* / Q000648:ultrastructure; D002417:Cattle; D002809:Chondroitin Sulfates / Q000737:chemistry / Q000378:metabolism; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism*; D024042:Collagen Type I / Q000737:chemistry / Q000378:metabolism; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D020007:Implants, Experimental; D008422:Materials Testing; D016348:Osseointegration / Q000502:physiology*; D013499:Surface Properties; D013552:Swine; D014025:Titanium / Q000737:chemistry*
chemical_list: C525809:BMP4 protein, human; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins; D020099:Coated Materials, Biocompatible; D024042:Collagen Type I; D002809:Chondroitin Sulfates; D014025:Titanium