title: Heterophilic binding of the adhesion molecules poliovirus receptor and immunoglobulin superfamily 4A in the interaction between mouse spermatogenic and Sertoli cells.
abstract: The cell adhesion protein immunoglobulin superfamily 4A (IGSF4A) is expressed on the surfaces of spermatogenic cells in the mouse testis. During spermatogenesis, IGSF4A is considered to bind to the surface of Sertoli cells in a heterophilic manner. To identify this unknown partner of IGSF4A, we generated rat monoclonal antibodies against the membrane proteins of mouse Sertoli cells grown in primary culture. Using these monoclonal antibodies, we isolated a clone that immunostained Sertoli cells and reacted with the product of immunoprecipitation of the homogenate of mouse testis with anti-IGSF4A antibody. Subsequently, to identify the Sertoli cell membrane protein that is recognized by this monoclonal antibody, we performed expression cloning of a cDNA library from the mouse testis. As a result, we identified poliovirus receptor (PVR), which is another IGSF-type cell adhesion molecule, as the binding partner of IGSF4A. The antibodies raised against PVR and IGSF4A immunoprecipitated both antigens in the homogenate of mouse testis. Immunoreactivity for PVR was present in Sertoli cells but not in spermatogenic cells at all stages of spermatogenesis. Overexpression of PVR in TM4, a mouse Sertoli cell line, increased more than three-fold its capacity to adhere to Tera-2, which is a human cell line that expresses IGSF4A. These findings suggest that the heterophilic binding of PVR to IGSF4A is responsible, at least in part, for the interaction between Sertoli and spermatogenic cells during mouse spermatogenesis.
authors.affiliation: Department of Histology and Embryology, Graduate School of Medical Science, Kanazawa University, 13-1 Takara-machi, Kanazawa 920-8640, Japan. twaka@basic.m.kanazawa-u.ac.jp
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Wakayama
authors.forename: Yoshimichi
authors.initials: Y
authors.lastname: Sai
authors.forename: Akihiko
authors.initials: A
authors.lastname: Ito
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kato
authors.forename: Miho
authors.initials: M
authors.lastname: Kurobo
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Murakami
authors.forename: Emi
authors.initials: E
authors.lastname: Nakashima
authors.forename: Akira
authors.initials: A
authors.lastname: Tsuji
authors.forename: Yukihiko
authors.initials: Y
authors.lastname: Kitamura
authors.forename: Shoichi
authors.initials: S
authors.lastname: Iseki
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D005260:Female; D007136:Immunoglobulins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008817:Mice, Mutant Strains; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism*; D012708:Sertoli Cells / Q000378:metabolism*; D013087:Spermatids / Q000166:cytology / Q000378:metabolism*; D013091:Spermatogenesis / Q000502:physiology; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C498598:Cadm1 protein, mouse; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules; D007136:Immunoglobulins; D008565:Membrane Proteins; D011991:Receptors, Virus; D025521:Tumor Suppressor Proteins; C066100:poliovirus receptor