title: Structure of archaeal glyoxylate reductase from Pyrococcus horikoshii OT3 complexed with nicotinamide adenine dinucleotide phosphate.
abstract: Glyoxylate reductase catalyzes the NAD(P)H-linked reduction of glyoxylate to glycolate. Here, the 1.7 A crystal structure of glyoxylate reductase from the hyperthermophilic archaeon Pyrococcus horikoshii OT3 complexed with nicotinamide adenine dinucleotide phosphate [NADP(H)] determined by the single-wavelength anomalous dispersion (SAD) method is reported. The monomeric structure comprises the two domains typical of NAD(P)-dependent dehydrogenases: the substrate-binding domain (SBD) and the nucleotide-binding domain (NBD). The crystal structure and analytical ultracentrifugation results revealed dimer formation. In the NADP(H)-binding site, the pyrophosphate moiety and the 2'-phosphoadenosine moiety are recognized by the glycine-rich loop (residues 157-162) and by loop residues 180-182, respectively. Furthermore, the present study revealed that P. horikoshii glyoxylate reductase contains aromatic clusters and has a larger number of ion pairs and a lower percentage of hydrophobic accessible surface area than its mesophilic homologues, suggesting its thermostability mechanism.
authors.affiliation: Protein Research Group, RIKEN Genomic Sciences Center, Tsurumi, Yokohama 230-0045, Japan.
authors.forename: Seiko
authors.initials: S
authors.lastname: Yoshikawa
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Arai
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Kinoshita
authors.forename: Tomomi
authors.initials: T
authors.lastname: Uchikubo-Kamo
authors.forename: Taisuke
authors.initials: T
authors.lastname: Wakamatsu
authors.forename: Ryogo
authors.initials: R
authors.lastname: Akasaka
authors.forename: Ryoji
authors.initials: R
authors.lastname: Masui
authors.forename: Takaho
authors.initials: T
authors.lastname: Terada
authors.forename: Seiki
authors.initials: S
authors.lastname: Kuramitsu
authors.forename: Mikako
authors.initials: M
authors.lastname: Shirouzu
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yokoyama
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry*; D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000737:chemistry*; D001665:Binding Sites; D019281:Dimerization; D008969:Molecular Sequence Data; D009249:NADP / Q000737:chemistry*; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D043902:Pyrococcus horikoshii / Q000201:enzymology*; D016415:Sequence Alignment
chemical_list: D019843:Archaeal Proteins; D009249:NADP; D000429:Alcohol Oxidoreductases; C020830:glyoxylate reductase; C025665:glyoxylate reductase (NADP+)