Label-free biosensor: a novel phage-modified Light Addressable Potentiometric Sensor system for cancer cell monitoring.
Early diagnosis has become the most important factor influencing the cancer's curing efficiency in clinical medicine. Here we present a new way for detection of cancer markers and cancer cells based on phage-modified Light Addressable Potentiometric Sensor (phage-LAPS). Phages were immobilized on the Si(3)N(4) chip surfaces covalently. Using the back-illuminating system, this phage-LAPS is tested to detect human phosphatase of regenerating liver-3 (hPRL-3) in the concentrations of 0.04-400 nM, and the mammary adenocarcinoma cell (MDAMB231) in the concentrations of 0-105 mL. The maximum responding signal is about 10 and 60 microV, respectively. The results demonstrated that the system was more applicable to detection of cancer cell than that of cancer biomarkers. This work might show potential application in clinic assays of cancer and the study of phage-cell interaction.
Jia
Yunfang
Y
Department of Microelectronics, Nankai University, Tianjin 300071, China. xzfeng@nankai.edu.cn
Qin
Ming
M
Zhang
Hongkai
H
Niu
Wencheng
W
Li
Xiao
X
Wang
Likai
L
Li
Xin
X
Bai
Yunpeng
Y
Cao
Youjia
Y
Feng
Xizeng
X
D000230:Adenocarcinoma / Q000175:diagnosis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D001943:Breast Neoplasms / Q000175:diagnosis*; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D008027:Light; D009363:Neoplasm Proteins / Q000032:analysis*; D019151:Peptide Library; D011199:Potentiometry / Q000295:instrumentation* / Q000379:methods; D017027:Protein Tyrosine Phosphatases / Q000032:analysis*; D012680:Sensitivity and Specificity
D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D019151:Peptide Library; C484730:PTP4A3 protein, human; D017027:Protein Tyrosine Phosphatases