High level expression of STAG1/PMEPA1 in an androgen-independent prostate cancer PC3 subclone.
In this paper, we describe the isolation and characterization of two PC3 subclones. One subclone, mr, showed an epithelial phenotype, the other, M1, showed a sarcomatous morphology. Transplanted into nude mice, mr developed tumors at a dramatically faster rate than M1. Comparing the two subclones, differentially expressed genes were identified, including E-cadherin, IL-8 and STAG1/PMEPA1. These genes were expressed at higher levels in mr than in M1.
Hirokawa
Yoshifumi S
YS
Department of Pathological Oncology, Institute of Molecular and Experimental Medicine, Faculty of Medicine, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie, 514-8507, Japan. ultray2k@clin.medic.mie-u.ac.jp
Takagi
Akimitsu
A
Uchida
Katsunori
K
Kozuka
Yuji
Y
Yoneda
Misao
M
Watanabe
Masatoshi
M
Shiraishi
Taizo
T
D000728:Androgens / Q000378:metabolism*; D000818:Animals; D015703:Antigens, CD; D015820:Cadherins / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002999:Clone Cells; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D040521:Subcutaneous Tissue / Q000473:pathology; D023041:Xenograft Model Antitumor Assays
D000728:Androgens; D015703:Antigens, CD; C513384:CDH1 protein, human; C508606:CXCL8 protein, human; D015820:Cadherins; D016209:Interleukin-8; D009687:Nuclear Proteins; C108522:STAG1 protein, human