System for inducible expression of cre-recombinase from the Foxa2 locus in endoderm, notochord, and floor plate.
We targeted the reverse tetracycline controlled transactivator (rtTA) to the Foxa2 locus (Foxa2(ITA)) to generate a system for regulating Cre-recombinase activity within Foxa2 expression domains, including the endoderm, notochord, and floor plate of early mouse embryos. The use of an internal ribosomal entry site to obtain rtTA expression preserves Foxa2 function of the targeted allele. Cre activity with this system reflects the level of endogenous Foxa2 activity and is also tightly controlled by doxycycline. The location of Cre activity within the broader Foxa2 expression domain can be restricted by altering the timing of doxycycline administration. Isolated floor plate expression can be obtained in this manner. This system will provide a useful tool for manipulating gene expression in endoderm, notochord, and floor plate, all of which are tissues with important structural and patterning functions during embryogenesis.
Frank
Deborah U
DU
Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, Utah, USA.
Elliott
Sarah A
SA
Park
Eon Joo
EJ
Hammond
Jennetta
J
Saijoh
Yukio
Y
Moon
Anne M
AM
D000818:Animals; D002490:Central Nervous System / Q000196:embryology / Q000378:metabolism*; D004318:Doxycycline / Q000494:pharmacology; D047108:Embryonic Development / Q000235:genetics; D004707:Endoderm / Q000378:metabolism*; D018628:Gene Dosage; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018390:Gene Targeting; D051542:Hepatocyte Nuclear Factor 3-beta / Q000235:genetics* / Q000378:metabolism; D019426:Integrases / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D008954:Models, Biological; D009672:Notochord / Q000378:metabolism*; D050436:Regulatory Elements, Transcriptional; D019076:Transgenes
C496503:Foxa2 protein, mouse; D051542:Hepatocyte Nuclear Factor 3-beta; C045073:Cre recombinase; D019426:Integrases; D004318:Doxycycline