title: Role of DNA methylation in stable gene repression.
abstract: A large fraction of the animal genome is maintained in a transcriptionally repressed state throughout development. By generating viable Dnmt1(-)(/)(-) mouse cells we have been able to study the effect of DNA methylation on both gene expression and chromatin structure. Our results confirm that the underlying methylation pattern has a profound effect on histone acetylation and is the major effector of me-H3(K4) in the animal genome. We demonstrate that many methylated genes are subject to additional repression mechanisms that also impact on histone acetylation, and the data suggest that late replication timing may play an important role in this process.
authors.affiliation: Department of Cellular Biochemistry and Human Genetics, Hebrew University Medical School, Ein Kerem, 91120 Jerusalem, Israel.
authors.forename: Laura
authors.initials: L
authors.lastname: Lande-Diner
authors.forename: Jianmin
authors.initials: J
authors.lastname: Zhang
authors.forename: Ittai
authors.initials: I
authors.lastname: Ben-Porath
authors.forename: Ninette
authors.initials: N
authors.lastname: Amariglio
authors.forename: Ilana
authors.initials: I
authors.lastname: Keshet
authors.forename: Merav
authors.initials: M
authors.lastname: Hecht
authors.forename: Veronique
authors.initials: V
authors.lastname: Azuara
authors.forename: Amanda G
authors.initials: AG
authors.lastname: Fisher
authors.forename: Gideon
authors.initials: G
authors.lastname: Rechavi
authors.forename: Howard
authors.initials: H
authors.lastname: Cedar
mesh_terms: D000107:Acetylation; D000818:Animals; D002843:Chromatin / Q000737:chemistry; D018899:CpG Islands; D004247:DNA / Q000737:chemistry; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics* / Q000502:physiology*; D019175:DNA Methylation; D004261:DNA Replication; D044127:Epigenesis, Genetic; D005347:Fibroblasts / Q000378:metabolism; D005786:Gene Expression Regulation; D006657:Histones / Q000737:chemistry; D051379:Mice; D008822:Mice, Transgenic; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D002843:Chromatin; D006657:Histones; D004247:DNA; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases; C000619381:Dnmt1 protein, mouse