[Production of interleukin-11 and interleukin-6 in cultured human gingival fibroblasts with the stimulation of lipopolysaccharides].
OBJECTIVE
To observe the effects of Porphyromonas gingivalis (Pg), Actinobacillus actinomycetemcomitans (Aa), Escherichia coli (Ec) lipopolysaccharides (LPS) on the production of IL-11 and IL-6 from healthy human gingival fibroblasts (HGF), and the effects of endogenous prostaglandin on HGF IL-11 and IL-6 production stimulated with the above LPS.


METHODS
HGF were stimulated with Pg-, Aa-, Ec-LPS of different concentrations (0.1, 1, 10 mg/L) for 24 h. And HGF were also stimulated with the combinations of 10 mg/L Pg-, Aa-, Ec-LPS and 10(-6) mol/L indomethacin respectively for 24 h. Levels of IL-11 and IL-6 in the supernatants were quantitated by ELISA.


RESULTS
LPS from Aa, Ec, at the concentration of 10 mg/L and from Pg at the concentrations 1, 10 mg/L significantly augmented IL-11 production by HGF. IL-6 production was also significantly increased by stimulation with Aa-LPS at concentrations 1, 10 mg/L and with Ec-, Pg-LPS at concentrations 0.1, 1, 10 mg/L. In addition, IL-11 production was lower than IL-6 production by HGF stimulated with LPS. Indomethacin significantly inhibited IL-6 and IL-11 production in LPS-stimulated HGF.


CONCLUSIONS
Aa-, Pg-, Ec-LPS may significantly increase IL-11 and IL-6 level in the supernatants of HGF, and endogenous prostaglandin may upregulate IL-11 and IL-6 production in LPS-stimulated HGF.
He
Lu
L
Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China. helubj@tom.com
Nagasawa
Toshiyuki
T
Ishikawa
Isao
I
D016976:Aggregatibacter actinomycetemcomitans / Q000737:chemistry; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000737:chemistry; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D005881:Gingiva / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D017370:Interleukin-11 / Q000096:biosynthesis*; D015850:Interleukin-6 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D016966:Porphyromonas gingivalis / Q000737:chemistry
D017370:Interleukin-11; D015850:Interleukin-6; D008070:Lipopolysaccharides; D007213:Indomethacin