[Induction of hypoxia-inducible factor-1alpha in two kinds of rats asphyxiation death models].
OBJECTIVE
To investigate the expression of hypoxia-inducible factor 1-alpha (HIF1-alpha) in the heart, lung, liver and kidney in rats died of two typical models of asphyxia.


METHODS
Two asphyxia models were made and tissue samples of the dead rats were collected from different groups at various postmortem duration. The expression and the changes of HIF1-alpha in various tissues were examined by immunohistochemistry and image analysis techniques. Results Significant expression of HIF1-alpha was observed in the myocardial fibers, kidney cells, liver cells and lung cells in both asphyxia models, but not in the control group. The expression of HIF1-alpha in various tissues in the rat died of nitrogen gas breathing was found in the nuclei at 0 hour and the expression level decreased gradually thereafter. The HIF1-alpha expression level and duration in various tissues of the rat died of hanging were higher and longer than that of the former group, with a peak of the expression level observed 6 hours after death, and then started to decline in all tissues except the heart where the expression still showed an increase 24 hours after death. The control groups showed a steady expression in the cytoplasm but not in the nuclei.


CONCLUSIONS
HIF1-alpha appears to be a valuable biomarker in the diagnosis of asphyxia within 24 hours after death.
Zhang
Bei-lei
BL
Department of Forensic Biology, West China School of Preclinical and Forensic medicine, Sichuan Univesity, Chengdu 610041, China. xiangzecai@gmail.com
Yang
Zhi-hui
ZH
Ran
Peng
P
Liang
Wei-bo
WB
Zhou
Bin
B
Zhang
Geng-qian
GQ
Lu
Mei-li
ML
Zhang
Lin
L
D000818:Animals; D001237:Asphyxia / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D020925:Hypoxia-Ischemia, Brain / Q000209:etiology / Q000378:metabolism*; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D008297:Male; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D009584:Nitrogen / Q000506:poisoning; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D009584:Nitrogen