Structure and function of bacterial kid-kis and related toxin-antitoxin systems.
Toxin-antitoxin systems were discovered as plasmid auxiliary maintenance cassettes. In recent years, an increasing amount of structural and functional information has become available about the proteins involved, allowing the understanding of bacterial cell growth inhibition by the toxins on a molecular level. A well-studied TA system is formed by the proteins Kid and Kis, encoded by the parD operon of the Escherichia coli plasmid R1. The toxicity of Kid has been related to its endoribonuclease activity, which is counteracted by binding of the antitoxin Kis at the proposed active site. In this review, the structural studies on the Kid-Kis system are compared to those of three related toxin-antitoxin systems: MazF-MazE, CcdB-CcdA and RelE-RelB.
Kamphuis
Monique B
MB
Bijvoet Center for Biomolecular Research, Department of NMR Spectroscopy, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
Monti
Maria Chiara
MC
van den Heuvel
Robert H H
RH
López-Villarejo
Juan
J
Díaz-Orejas
Ramón
R
Boelens
Rolf
R
D000595:Amino Acid Sequence; D000992:Antitoxins; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism*; D001427:Bacterial Toxins / Q000737:chemistry* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism; D008024:Ligands; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011815:R Factors / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D016415:Sequence Alignment; D013329:Structure-Activity Relationship
D000992:Antitoxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D029968:Escherichia coli Proteins; C068353:Kid protein, Bacteria; C068354:Kis protein, Bacteria; D008024:Ligands; D012313:RNA