PC12 cells express IL-1 receptor type I and response to IL-1beta stimulation.
PC12 cell line has been widely used in a diverse array of neurophysiological studies including in exploration of oxygen-sensing mechanism. In present study, we first identified with immunocytochemistry and Western blot methods that interleukin-1 receptor type I was expressed in the PC12 cells. We then demonstrated with patch clamping technique that extracellular application of IL-1beta dose-dependently inhibited the outward voltage-dependent and TEA-sensitive potassium currents (I(K)) in the PC12 cells, and pre-incubation with the interleukin-1 receptor antagonist almost completely abolished this inhibitory effect. In addition, application of IL-1beta shifted steady-state inactivation of I(K) in hyperpolarizing direction, but did not alter its steady-state activation. Furthermore, IL-1beta-induced inhibition of I(K) led to a membrane depolarization and a transient increase of [Ca(2+)](i) in PC12 cells. Taking together, the present study elucidates that PC12 cells bear interleukin-1 receptor and response to IL-1beta stimulation.
Shu
Hai-Feng
HF
Institute of Neurosciences, The Fourth Military Medical University, Xi'an 710032, PR China.
Wang
Bai-Ren
BR
Bi
Hui
H
Pei
Jian-Ming
JM
Wang
Xi
X
Fan
Juan
J
Ju
Gong
G
D000704:Analysis of Variance; D000818:Animals; D002118:Calcium / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D015870:Gene Expression / Q000187:drug effects*; D053590:Interleukin 1 Receptor Antagonist Protein / Q000494:pharmacology; D053583:Interleukin-1beta / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D016716:PC12 Cells / Q000187:drug effects / Q000378:metabolism; D018408:Patch-Clamp Techniques / Q000379:methods; D051381:Rats; D053573:Receptors, Interleukin-1 Type I / Q000378:metabolism*; D019789:Tetraethylammonium / Q000494:pharmacology
D053590:Interleukin 1 Receptor Antagonist Protein; D053583:Interleukin-1beta; D053573:Receptors, Interleukin-1 Type I; D019789:Tetraethylammonium; D002118:Calcium