title: Stimulation of cysteinyl leukotriene production in mast cells by heat shock and acetylsalicylic acid.
abstract: Immunoglobulin (Ig) E-dependent activation of mast cells is central to the allergic response. The engagement of IgE-occupied receptors initiates a series of molecular events that causes the release of preformed, and de novo synthesis of, allergic mediators. Cysteinyl leukotrienes are able to contract airway smooth muscle and increase mucus secretion and vascular permeability and recruit eosinophils. Mast cells have also recently been recognized as active participants in innate immune responses. Heat stress can modulate innate immunity by inducing stress proteins such as heat-shock proteins (HSPs). We previously demonstrated that treatment of mast cells with heat shock or acetylsalicylic acid results in an increase of TNF-alpha and IL-6 release. This effect was paralleled by expression of HSP70. In the current study, we further investigated the effects of heat shock and acetylsalicylic acid on the activation of mast cells and the release of cysteinyl leukotrienes. In mouse mast cells, derived from a culture of bone marrow cells, responsiveness to heat shock, acetylsalicylic acid and exogenous or endogenous HSP70 was monitored by measuring leukotriene C4 release. We show that after heat shock treatment and exposure to acetylsalicylic acid leukotriene production was increased. Moreover, exogenous rHSP70 also induced leukotriene production. Because it has been reported that leukotriene production in mast cells may be mediated by Toll like receptor (TLR) activation, and HSP70 also activates TLRs signaling, we further explored these issues by using mast cells that are not able to produce HSP70, i.e. heat shock factor-1 (HSF-1) knockout cells. We found that in HSF-1 knockout bone marrow derived mast cells, heat shock and acetylsalicylic acid failed to induce release of leukotrienes. Moreover, in wild type cells the surface expression of TLR4 was attenuated, whereas the intracellular expression was up-regulated. We conclude that heat shock and acetylsalicylic acid induce the production and release of heat shock proteins from mast cells, which in turn stimulate leukotriene synthesis through activation of TLR4.
authors.affiliation: Division of Pharmacology and Pathophysiology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, P O BOX 80082, 3508 TB Utrecht, The Netherlands.
authors.forename: Esmaeil
authors.initials: E
authors.lastname: Mortaz
authors.forename: Frank A
authors.initials: FA
authors.lastname: Redegeld
authors.forename: Kathy
authors.initials: K
authors.lastname: Dunsmore
authors.forename: Kelli
authors.initials: K
authors.lastname: Odoms
authors.forename: Hector R
authors.initials: HR
authors.lastname: Wong
authors.forename: Frans P
authors.initials: FP
authors.lastname: Nijkamp
authors.forename: Ferdi
authors.initials: F
authors.lastname: Engels
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001241:Aspirin / Q000494:pharmacology*; D015153:Blotting, Western; D002470:Cell Survival; D003545:Cysteine / Q000187:drug effects* / Q000378:metabolism; D005434:Flow Cytometry; D018840:HSP70 Heat-Shock Proteins / Q000187:drug effects / Q000378:metabolism* / Q000494:pharmacology; D018869:Heat-Shock Response; D017997:Leukotriene C4 / Q000096:biosynthesis / Q000378:metabolism; D015289:Leukotrienes / Q000378:metabolism; D008407:Mast Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D051195:Toll-Like Receptor 2 / Q000187:drug effects / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000187:drug effects* / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D018840:HSP70 Heat-Shock Proteins; D015289:Leukotrienes; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; C112381:cysteinyl-leukotriene; D017997:Leukotriene C4; D003545:Cysteine; D001241:Aspirin