Determination of oxytetracycline levels in rainbow trout serum on a biphenyl column using high-performance liquid chromatography.
We developed a simple and sensitive high-performance liquid chromatography method on a biphenyl column to determine oxytetracycline (OTC) levels in rainbow trout serum. The assay used deproteination, filtration, and subsequent separation on a reverse-phase biphenyl column, with UV detection at 355 nm. OTC (7.8-7.9 min) was completely resolved from structurally similar riboflavin (10.4-10.5 min), a common feed supplement. Estimated limits of detection and quantitation of OTC were 0.01 and 0.04 microg/mL, respectively. The average recovery for OTC was 102% with a R.S.D. of 8.34%. Calibration standards were linear from 0.01 to 10 microg/mL.
Miller
Ron A
RA
U.S. Food and Drug Administration, Center for Veterinary Medicine, Office of Research, 8401 Muirkirk Road, Laurel, MD 20708, USA. Ron.Miller@fda.hhs.gov
Reimschuessel
Renate
R
Carson
Mary C
MC
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D017686:Oncorhynchus mykiss; D010118:Oxytetracycline / Q000097:blood*; D012680:Sensitivity and Specificity
D010118:Oxytetracycline