title: Endothelin-3 and PRL levels in the maternal and fetal circulation at delivery.
abstract: OBJECTIVE
  The aim of the present cross-sectional study was to test the hypothesis that endothelin-3 (ET-3) is involved in PRL secretion via systemic hormonal interaction during labor.
  
  
  METHODS
  Fifty healthy pregnant women with singleton pregnancies were included in the present study. At delivery, blood samples were drawn from umbilical vein and artery. At the same time, a blood sample was obtained from a peripheral vein of the mother. In all blood samples, plasma ET-3 and serum PRL concentrations were determined. The main outcome measures were the differences between maternal peripheral blood, umbilical artery and vein in terms of ET-3 and PRL levels, and the associations between ET-3 and PRL levels.
  
  
  RESULTS
  ET-3 values (mean+/-SEM) in umbilical artery did not differ significantly from those in umbilical vein (4.94+/-0.27 vs 5.05+/-0.32 pg/ml) but were in both vessels significantly higher than in maternal vein (1.14+/-0.56 pg/ml, p<0.001). Serum PRL values showed similar patterns. There was a significant positive correlation of the ET-3 levels between umbilical artery and vein (r=0.906, p<0.001), but not between maternal peripheral venous blood and the umbilical vessels. Similar correlations were found for PRL values. However, no significant correlations were found between ET-3 and PRL levels in all vessels studied.
  
  
  CONCLUSIONS
  The present study demonstrates for the first time that ET-3 levels are higher in fetal than in maternal circulation at term. The lack of correlation between ET-3 and PRL levels suggests that ET-3 does not play an important endocrine role in the control of maternal and fetal PRL secretion during labor.
authors.affiliation: Department of Obstetrics and Gynecology, University of Thessalia, 22 Papakiriazi street, 41222 Larissa, Greece.
authors.forename: K
authors.initials: K
authors.lastname: Dafopoulos
authors.forename: A
authors.initials: A
authors.lastname: Boli
authors.forename: A
authors.initials: A
authors.lastname: Kallitsaris
authors.forename: A
authors.initials: A
authors.lastname: Malamitsi-Puchner
authors.forename: G
authors.initials: G
authors.lastname: Kollios
authors.forename: I E
authors.initials: IE
authors.lastname: Messinis
mesh_terms: D000328:Adult; D003430:Cross-Sectional Studies; D036861:Delivery, Obstetric; D019334:Endothelin-3 / Q000097:blood*; D005260:Female; D005312:Fetal Blood / Q000737:chemistry*; D006801:Humans; D007231:Infant, Newborn; D007743:Labor, Obstetric / Q000097:blood*; D008297:Male; D008431:Maternal-Fetal Exchange; D011247:Pregnancy; D011388:Prolactin / Q000097:blood*
chemical_list: D019334:Endothelin-3; D011388:Prolactin