title: Abnormality of CD4(+)CD25(+) regulatory T cells in idiopathic thrombocytopenic purpura.
abstract: OBJECTIVE
  The aim of this study was to explore the profile and function of CD4(+)CD25(+) regulatory T cells (Treg cells) in idiopathic thrombocytopenic purpura (ITP) patients.
  
  
  METHODS
  Treg cell numbers were analyzed by flow cytometric analysis in peripheral blood mononuclear cells collected from healthy donors or patients with ITP. Quantification of cell proliferation was assayed by an enzyme-linked immunosorbent assay kit, based on the measurement of BrdU incorporation during DNA synthesis.
  
  
  RESULTS
  The percentage of Treg cells was significantly decreased in ITP patients in active and non-remission state(5.79 +/- 1.22%) when compared with the patients in remission(11.63 +/- 4.56%) and to healthy subjects(12.68 +/- 3.59%). The suppressive activity of Treg cells in ITP patients was also found to be impaired.
  
  
  CONCLUSIONS
  These results suggest that decreased number and function of Treg cells might be one of mechanisms that cause immune regulation dysfunction in ITP.
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.forename: Man-Chiu
authors.initials: MC
authors.lastname: Poon
authors.forename: Zhibo
authors.initials: Z
authors.lastname: Han
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Gu
authors.forename: Maoqiang
authors.initials: M
authors.lastname: Xu
authors.forename: Hairong
authors.initials: H
authors.lastname: Jia
authors.forename: Renchi
authors.initials: R
authors.lastname: Yang
authors.forename: Zhong Chao
authors.initials: ZC
authors.lastname: Han
mesh_terms: D000293:Adolescent; D000328:Adult; D015704:CD4 Antigens / Q000032:analysis; D002455:Cell Division; D002478:Cells, Cultured / Q000276:immunology; D004261:DNA Replication; D005260:Female; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D053645:Interleukin-2 Receptor alpha Subunit / Q000032:analysis; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D011239:Prednisolone / Q000627:therapeutic use; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000188:drug therapy / Q000276:immunology* / Q000473:pathology; D012074:Remission Induction; D013103:Spherocytosis, Hereditary / Q000276:immunology / Q000473:pathology; D013154:Spleen / Q000276:immunology / Q000473:pathology; D050378:T-Lymphocytes, Regulatory / Q000737:chemistry / Q000276:immunology / Q000473:pathology*
chemical_list: D015704:CD4 Antigens; D007166:Immunosuppressive Agents; D053645:Interleukin-2 Receptor alpha Subunit; D011239:Prednisolone