title: A FAFLP system for the improved identification of plant-pathogenic and plant-associated species of the genus Pantoea.
abstract: The majority of Pantoea species are either plant-pathogenic or plant-associated and cause a wide variety of symptoms on a range of hosts. Identification of Pantoea species is difficult due to minor differences in phenotypic characteristics between them and related Enterobacteriaceae. Fluorescent amplified fragment length polymorphism (FAFLP) analysis was investigated for use as a rapid, molecular-based identification technique to the species level of the genus Pantoea. Following analysis of the band patterns generated by FAFLP, seven distinct clusters were observed, one for each validly published species of the genus. FAFLP has proven to be a rapid, reproducible identification technique for all species of the genus Pantoea.
authors.affiliation: Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria 0002, South Africa. Carrie.Brady@fabi.up.ac.za
authors.forename: Carrie
authors.initials: C
authors.lastname: Brady
authors.forename: Stephanus
authors.initials: S
authors.lastname: Venter
authors.forename: Ilse
authors.initials: I
authors.lastname: Cleenwerck
authors.forename: Marc
authors.initials: M
authors.lastname: Vancanneyt
authors.forename: Jean
authors.initials: J
authors.lastname: Swings
authors.forename: Teresa
authors.initials: T
authors.lastname: Coutinho
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D020636:Pantoea / Q000145:classification* / Q000235:genetics; D010935:Plant Diseases / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000032:analysis* / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S