title: Genotyping and quantitation of noroviruses in oysters from two distinct sea areas in Japan.
abstract: Norovirus (NV) is a causative agent of acute gastroenteritis in humans, and shellfishes including oysters act as major vehicles of the virus. To investigate the genetic characteristics of NVs, we collected 1,512 oysters for raw consumption between October 2002 and March 2005 from two distinct areas (area A: the Sanriku Sea area; area B: the Setouchi Sea area). We detected the capsid gene and subjected it to phylogenetic analysis. By further quantification of the copy number of the genome by using real-time PCR, the NV capcid gene was detected in approximately 5% of the oysters, and they showed wide diversity. Two percent of the oysters from area B showed relatively large number of NVs, i.e., over 100 copies of capsid gene/oyster, whereas this was not observed in area A. Most of the detected NVs from oysters and humans were genetically related when the capsid region was compared. These results suggested that NVs obtained from humans and those obtained from oysters showed a potential relationship to each other and that some populations of Japanese oysters accumulated a relatively large number of NVs.
authors.affiliation: Yamaguchi Prefectural Research Institute of Public Health, Yamaguchi, Japan. nishida.tomoko@pref.yamaguchi.lg.jp
authors.forename: Tomoko
authors.initials: T
authors.lastname: Nishida
authors.forename: Osamu
authors.initials: O
authors.lastname: Nishio
authors.forename: Masahiko
authors.initials: M
authors.lastname: Kato
authors.forename: Takehisa
authors.initials: T
authors.lastname: Chuma
authors.forename: Hirotomo
authors.initials: H
authors.lastname: Kato
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Iwata
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Kimura
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D005516:Food Microbiology; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007564:Japan; D029322:Norovirus / Q000235:genetics* / Q000302:isolation & purification; D010125:Ostreidae / Q000821:virology*; D010802:Phylogeny; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral