Concanavalin A induced expression of Toll-like receptors in murine peritoneal macrophages in vitro.
The differential expression of Toll-like receptors (TLRs 1-9) and their associated proteins in murine peritoneal macrophages in vitro, on treatment with plant lectin concanavalin A (Con A) has been investigated. It is observed that there is enhanced expression of TLRs 2-9 and downstream molecules--MyD88, IRAK1, TRAF6 and IRF3 in murine peritoneal macrophages on in vitro treatment with Con A. Pretreatment of macrophages with inhibitors of JNK, p38, p42/44 and NF-kappaB, significantly decreased the Con A induced expression of TLRs. When cells are pre-treated with Con A and subsequently treated with TLR ligands--Zymosan A, PolyI:C, LPS, CpG DNA, there is enhanced production of proinflammatory cytokines (TNF-alpha, IL-1beta, IL-12 and IFN-gamma,), nitric oxide and iNOS expression in murine peritoneal macrophages. The results suggest that treatment of macrophages with Con A renders them more susceptible to subsequent activation and induction of proinflammatory cytokines and nitric oxide production by different TLR ligands.
Sodhi
Ajit
A
School of Biotechnology, Banaras Hindu University, Varanasi, India. ajit.sodhi@lycos.com
Tarang
Shikha
S
Kesherwani
Varun
V
D000818:Animals; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology*; D005260:Female; D050838:Interferon Regulatory Factor-3 / Q000096:biosynthesis; D053592:Interleukin-1 Receptor-Associated Kinases / Q000096:biosynthesis; D018664:Interleukin-12 / Q000096:biosynthesis; D053583:Interleukin-1beta / Q000096:biosynthesis; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D053594:Myeloid Differentiation Factor 88 / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D048029:TNF Receptor-Associated Factor 6 / Q000096:biosynthesis; D051193:Toll-Like Receptors / Q000096:biosynthesis* / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D015854:Up-Regulation
D050838:Interferon Regulatory Factor-3; D053583:Interleukin-1beta; C494233:Irf3 protein, mouse; C507780:Myd88 protein, mouse; D053594:Myeloid Differentiation Factor 88; D012333:RNA, Messenger; D048029:TNF Receptor-Associated Factor 6; D051193:Toll-Like Receptors; D014409:Tumor Necrosis Factor-alpha; D003208:Concanavalin A; D018664:Interleukin-12; D053592:Interleukin-1 Receptor-Associated Kinases; C507769:Irak1 protein, mouse