A high-resolution structure of ligand-free human glutamate carboxypeptidase II.
Human glutamate carboxypeptidase II (GCPII; EC 3.4.17.21) is an established marker for prostate-cancer diagnosis as well as a candidate therapeutic target for the treatment of diverse pathologies that involve glutamatergic transmission. Structural data on GCPII are thus valuable for the design and optimization of GCPII-specific inhibitors and diagnostic probes. The currently available structure of ligand-free GCPII was refined to a resolution of 3.5 A. This work reports the structure of the protein refined to 1.65 A resolution, with crystallographic values of R = 0.207 and R(free) = 0.228. The new structure extends the resolution appreciably and the new model based on this data shows significant differences when compared with the previously published model.
Barinka
Cyril
C
National Cancer Institute at Frederick, Center for Cancer Research, Frederick, MD 21702, USA. cyril@ncifcrf.gov
Starkova
Jana
J
Konvalinka
Jan
J
Lubkowski
Jacek
J
D000818:Animals; D000954:Antigens, Surface / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites / Q000502:physiology; D003460:Crystallization; D018360:Crystallography, X-Ray; D004330:Drosophila; D043425:Glutamate Carboxypeptidase II / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008024:Ligands; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism
D000954:Antigens, Surface; D008024:Ligands; D011994:Recombinant Proteins; C473848:FOLH1 protein, human; D043425:Glutamate Carboxypeptidase II