Expression of Escherichia coli heat-labile enterotoxin b subunit (LTB) in carrot (Daucus carota L.).
We expressed the B subunit of enterotoxigenic Escherichia coli heat-labile enterotoxin (LTB) encoded by a synthetic codon-optimized gene in carrot. An Agrobacterium-mediated transformation method was used. Thirty independent transgenic lines were regenerated via somatic embryogenesis after 6 months in culture and were transferred to a greenhouse. GM1-ELISA assay was used to assess LTB protein content in mature taproots. Some transgenic lines expressed LTB up to 0.3% of the total soluble protein, which is tenfold higher than the expression levels reported earlier using the native bacterial gene in plants. Immunological assay confirmed proper assembly of the pentameric complex and in vitro activity of the recombinant LTB protein, suggesting that it can be functional in prevention of diarrhea.
Rosales-Mendoza
Sergio
S
División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José 2055, 78216 San Luis Potosí, S.L.P., Mexico.
Soria-Guerra
Ruth Elena
RE
de Jesús Olivera-Flores
María Teresa
MT
López-Revilla
Rubén
R
Argüello-Astorga
Gerardo Rafael
GR
Jiménez-Bremont
Juan Francisco
JF
García-de la Cruz
Ramón Fernando
RF
Loyola-Rodríguez
Juan Pablo
JP
Alpuche-Solís
Angel Gabriel
AG
D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism*; D018552:Daucus carota / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D004768:Enterotoxins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D030821:Plants, Genetically Modified
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C027538:heat-labile enterotoxin, E coli