The copper tolerance strategies and the role of antioxidative enzymes in three plant species grown on copper mine.
This study was undertaken to identify the strategies and the status of antioxidant enzyme activities involved in three plant species tolerance against Cu-toxicity in copper mine. The following methods were used for evaluations in three wild type species; Datura stramonium, Malva sylvestris and Chenopodium ambrosioides. The level of chlorophyll and the activities of superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase (CAT) by spectrometry, malondialdehyde (MDA) and dityrosine by HPLC and the levels of Cu in tissues and soils by atomic absorption spectrometry (AAS). Analysis showed that total and available copper were at toxic levels for plants growing on contaminated soil (zone 1). However, there were not any visual and conspicuous symptoms of Cu toxicity in plant species. Among three species, excess copper was transferred only into the D. stramonium and C. ambrosioides tissues. The C. ambrosioides accumulated Cu in roots and then in leaves, in which the leaves chloroplasts stored Cu around two times of vacuoles. In D. stramonium most of Cu was accumulated in leaves in which the storage rate in vacuoles and chloroplasts were 42% and 8%, respectively. In zone 1, the chlorophyll levels increased significantly in leaves of C. ambrosioides with respect to the same plant growing on uncontaminated soil (zone 2). There was insignificant decrease in chlorophyll content of D. stramonium leaves, collected from zone 1 with respect to zone 2. The D. stramonium and C. ambrosioides in zone 1, both revealed significant increase in their tissues antioxidant enzyme activities in comparison with the same samples of zone 2. There was significant elevation in oxidative damage biomarkers; MDA and dityrosine, when the aerial parts of D. stramonium in zone 1 were compared with the same parts of zone 2. We concluded that there were different tolerance strategies in studied plant species that protected them against copper toxicity. In M. sylvestris, exclusion of Cu from the roots or its stabilization in the soil restricted Cu toxicity effects. On the other hand D. stramonium and C. ambrosioides, elevated their antioxidative enzyme activities in response to cu-toxicity. In addition, the species D. stramonium accumulated excess of Cu in leaves vacuoles.
Boojar
Massod Mashhadi Akbar
MM
Department of Biology, University of Tarbiat Moalem, No. 49, Dr. Mofateh Avenue, Tehran, P.O. Box 15614, Iran. aboojar@yahoo.com
Goodarzi
Faranak
F
D000975:Antioxidants / Q000378:metabolism*; D018533:Biomass; D027462:Chenopodium / Q000201:enzymology; D002734:Chlorophyll / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism; D003300:Copper / Q000032:analysis / Q000633:toxicity*; D032302:Datura / Q000201:enzymology; D050356:Lipid Metabolism / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D028501:Malva / Q000201:enzymology; D008906:Mining; D018384:Oxidative Stress / Q000502:physiology; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry; D010944:Plants / Q000201:enzymology*; D012987:Soil / Q000032:analysis; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism; D014617:Vacuoles / Q000378:metabolism
D000975:Antioxidants; D010940:Plant Proteins; D012987:Soil; D002734:Chlorophyll; D014443:Tyrosine; D008315:Malondialdehyde; D003300:Copper; C007543:dityrosine