title: Safety assessment of transgenic Bacillus thuringiensis with VIP insecticidal protein gene by feeding studies.
abstract: The aim of this study was to evaluate the toxicology safety of a genetically modified (GM) Bacillus thuringiensis with vegetative insecticidal protein (VIP) gene. Acute and subacute toxicity studies by using its powder preparation were conducted in Wistar rats. The result of the acute study showed the no-observable-adverse-effect level (NOAEL) of this GM B. thuringiensis powder preparation was greater than 5000 mg/kg body weight (BW). In the subacute study, the data analysis of body weight gain, food and water consumptions, clinical observations, haematology, serum biochemistry, organ weight ratios and histopathological findings did not show significant differences between control and treated groups. These results proved the NOAEL of this GM B. thuringiensis powder preparation in subacute test was greater than 5000 mg/kg BW. Since both the acute and subacute oral toxicity were not detected at the highest dose recommended by OECD guidelines, this GM B. thuringiensis could be generally regarded as safe for use in bio-pesticide industry.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Donghai
authors.initials: D
authors.lastname: Peng
authors.forename: Shouwen
authors.initials: S
authors.lastname: Chen
authors.forename: Lifang
authors.initials: L
authors.lastname: Ruan
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Ziniu
authors.initials: Z
authors.lastname: Yu
authors.forename: Ming
authors.initials: M
authors.lastname: Sun
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000235:genetics / Q000633:toxicity*; D019963:Clinical Chemistry Tests; D004305:Dose-Response Relationship, Drug; D004326:Drinking / Q000187:drug effects; D004435:Eating / Q000187:drug effects; D005260:Female; D006403:Hematologic Tests; D007306:Insecticides / Q000008:administration & dosage / Q000633:toxicity*; D008136:Longevity / Q000187:drug effects; D008297:Male; D019055:No-Observed-Adverse-Effect Level; D009929:Organ Size / Q000187:drug effects; D030781:Organisms, Genetically Modified; D010572:Pest Control, Biological; D011208:Powders; D051381:Rats; D017208:Rats, Wistar; D018675:Toxicity Tests / Q000379:methods*; D015430:Weight Gain / Q000187:drug effects
chemical_list: D001426:Bacterial Proteins; D007306:Insecticides; D011208:Powders; C447139:Vip3A protein, Bacillus thuringiensis