Possible involvement of oxidative stress in salivary gland of patients with Sjogren's syndrome.
OBJECTIVE
To determine the involvement of oxidative stress in the salivary gland of patients with Sjogren's syndrome (SS).


METHODS
Oxidative damage to the gland was measured by 8-hydroxy-2'-deoxyguanosine (8-OHdG) and hexanoyl-lysine (HEL) using the SS saliva. In addition, lactate dehydrogenase (LDH) and mitochondrial glutamic-oxaloacetic transaminase (m-GOT), both general markers for cell damage, were also analyzed.


RESULTS
Increased levels of 8-OHdG and HEL were found in the saliva of SS patients, but not in that of patients with other salivary gland dysfunction or of healthy individuals. Levels of LDH and m-GOT were significantly correlated with 8-OHdG and HEL levels, respectively. Furthermore, the increased levels of 8-OHdG and HEL were also correlated in the SS saliva.


CONCLUSIONS
These findings suggested the involvement of oxidative stress in glandular tissue destruction in SS. It was indicated that the detection of 8-OHdG and HEL in the saliva may become a useful tool for the diagnosis of SS.
Ryo
Koufuchi
K
Department of Pathology, Tsurumi University School of Dental Medicine, Yokohama, Japan.
Yamada
Hiroyuki
H
Nakagawa
Yoichi
Y
Tai
Yoshinori
Y
Obara
Kumi
K
Inoue
Hiroko
H
Mishima
Kenji
K
Saito
Ichiro
I
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001219:Aspartate Aminotransferases / Q000032:analysis; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000032:analysis; D005260:Female; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008875:Middle Aged; D008928:Mitochondria / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology*; D012463:Saliva / Q000737:chemistry; D012469:Salivary Glands / Q000378:metabolism*; D012859:Sjogren's Syndrome / Q000175:diagnosis* / Q000378:metabolism*
D000080242:8-Hydroxy-2'-Deoxyguanosine; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D003849:Deoxyguanosine