title: Enhanced absorption of anthocyanins after oral administration of phytic acid in rats and humans.
abstract: Many studies on the bioavailability of polyphenols have been reported. However, the relative urinary excretions of AC are also low, ranging from 0.004% to 0.1%. By contrast, other polyphenols show higher urinary excretion levels. Here, we studied the enhancing effects of phytic acid (IP6) on absorption of blackcurrant anthocyanins (BCAs) in rats and humans. In rats after oral administration of BCAs (as 241 mg of AC/kg body weight) in IP6 (0%, 0.25%, 0.5%, 1%, 2.5%) solution, the ACs recovery in urine was increased dependent on IP6 dose. These results suggest that the IP6 enhances gastrointestinal absorption of ACs. At the further analysis of IP6 enhancement effect in rat, whereas BCAs were normally passed through the stomach and duodenum within 2 h, in IP6 group, after 2-6 h post-administration, stomach and jejunum content's weights were specifically heavy, and large amounts of ACs were also detected in stomach, duodenum, and jejunum. These results suggested that the mixture of BCAs and IP6 reduced the gastrointestinal motility. Prolongation of ACs residue in gastrointestinal tract then caused the enhancing effects of IP6 on absorption of AC. In the human study, each subject was orally administrated a BCA beverage containing BCA concentrate (AC 4 mg/kg body weight), 1% of IP6, and 1% of sodium citrate as a pH stabilizer. Both the plasma level and the urinary excretion of AC were increased as compared to BCA administration without IP6. AC intake with IP6 may increase the bioavailability of AC to the comparative level as other polyphenols. Yet, phytic acid, being a strong chelator of important minerals, contributes to mineral deficiencies. An interference with iron uptake has been reported. Safety tests are therefore necessary before high dose IP6 can be used in foods.
authors.affiliation: Food and Health R&D Laboratories, Meiji Seika Kaisha, Ltd., 5-3-1, Chiyoda, Sakado-shi, Saitama 350-0289, Japan. hitoshi_matsumoto@meiji.co.jp
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Matsumoto
authors.forename: Kyoko
authors.initials: K
authors.lastname: Ito
authors.forename: Kumiko
authors.initials: K
authors.lastname: Yonekura
authors.forename: Takanori
authors.initials: T
authors.lastname: Tsuda
authors.forename: Takashi
authors.initials: T
authors.lastname: Ichiyanagi
authors.forename: Masao
authors.initials: M
authors.lastname: Hirayama
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Konishi
mesh_terms: D000328:Adult; D000818:Animals; D000872:Anthocyanins / Q000493:pharmacokinetics* / Q000652:urine; D001682:Biological Availability; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007408:Intestinal Absorption / Q000187:drug effects*; D008297:Male; D010833:Phytic Acid / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000872:Anthocyanins; D010833:Phytic Acid