[Microwave-assisted extraction of flavonoids in Mulberry leaf and root-bark].
OBJECTIVE
To extract flavonoids in Mulberry leaf and White Mulberry root-bark by microuave-assisted method.


METHODS
An orthogonal table was used to find the best procedure for microwave-assisted extraction (MAE) of flavonoids. The advantages of MAE were explored by comparing with the commonly used extraction methods. The different contents of flavonoids in Mulberry Leaf and Mulberry Root-bark were also compared.


RESULTS
The best condition for procedure was extracting 20 min at 60 degrees C with 12 times of 70% ethanol. Among all factors, the ethanol concentration was significant This experiment implied that flavonoids in Mulberry leaf were 9 times as that in Mulberry Root-bark probably. Moreover, the flavonoids extracted from Mulberry Leaf and White Mulberry root-bark by MAE were 55% and 32% more than that from commonly used extraction method individually.


CONCLUSIONS
MAE is advantageous than commonly used extraction method. It can be used for batch production.
Chen
Jing-jing
JJ
College of Pharmaceutical Science, Zhejiang University, Hangzhou 310031, China.
Li
Xiang-rong
XR
D000704:Analysis of Variance; D000431:Ethanol; D005419:Flavonoids / Q000032:analysis / Q000302:isolation & purification*; D008872:Microwaves; D031625:Morus / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012997:Solvents; D013678:Technology, Pharmaceutical / Q000379:methods*; D013995:Time
D005419:Flavonoids; D012997:Solvents; D000431:Ethanol