Endothelial cells derived from human embryonic stem cells form durable blood vessels in vivo.
We describe the differentiation of human embryonic stem (hES) cells into endothelial cells using a scalable two-dimensional method that avoids an embryoid-body intermediate. After transplantation into severe combined immunodeficient (SCID) mice, the differentiated cells contributed to arborized blood vessels that integrated into the host circulatory system and served as blood conduits for 150 d.
Wang
Zack Z
ZZ
Maine Medical Center Research Institute, Scarborough, Maine 04074, USA.
Au
Patrick
P
Chen
Tong
T
Shao
Ying
Y
Daheron
Laurence M
LM
Bai
Hao
H
Arzigian
Melanie
M
Fukumura
Dai
D
Jain
Rakesh K
RK
Scadden
David T
DT
D000818:Animals; D001808:Blood Vessels / Q000166:cytology* / Q000254:growth & development*; D002454:Cell Differentiation; D053595:Embryonic Stem Cells / Q000166:cytology*; D042783:Endothelial Cells / Q000166:cytology* / Q000502:physiology; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D014741:Video Recording