Evaluation of alloreactivity in responder-stimulator pairs by determination of gamma interferon-producing cells and cytotoxic-T-lymphocyte precursor frequencies.
We used the enzyme-linked immunospot (ELISPOT) assay and the cytotoxic-T-lymphocyte precursor frequency assay to evaluate alloreactivity in responder-stimulator pairs. High frequencies of responder cells among cells from HLA-mismatched pairs and low frequencies among cells from pairs of siblings with identical HLA types were detected by both assays. The ELISPOT assay thus illustrated the helper and cytotoxic-T-cell response to allogeneic HLA antigens.
Sizzano
Federico
F
Transplantation Immunology, San Giovanni Battista Hospital, Turin, Italy. fsizzano@molinette.piemonte.it
Dametto
Ennia
E
Amoroso
Antonio
A
D000818:Animals; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D008213:Lymphocyte Activation / Q000276:immunology*; D018655:Lymphocyte Count; D051379:Mice; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology* / Q000378:metabolism
D007371:Interferon-gamma