Target-directed proteolysis in vivo.
The experimental problems associated with in vivo studies of essential proteins or integral membrane proteins have triggered geneticists to generate novel approaches that have often led to insights of general relevance (Shuman and Silhavy, 2003). In order to extend the experimental portfolio, we developed target-directed proteolysis (TDP), an in vivo method allowing structural and functional characterization of target proteins in living cells. TDP is based on the activity of the highly sequence-specific NIa protease from tobacco etch virus. When its recognition site of seven residues is engineered into target proteins and NIa protease is expressed under tight promoter control, substrates can be conditionally processed while other cellular proteins remain unaffected. Applications include conditional inactivation as well as functional characterization of target proteins.
Eser
Markus
M
School of Biosciences, Cardiff University, Cardiff, Wales, UK.
Henrichs
Tanja
T
Boyd
Dana
D
Ehrmann
Michael
M
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004251:DNA Transposable Elements; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D006868:Hydrolysis; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D011506:Proteins; D014764:Viral Proteins; D010450:Endopeptidases