title: Sets of transposon-generated sequence-tagged mutants for structure-function analysis and engineering.
abstract: Various genetic strategies are available for the isolation of small, in-frame insertional mutants. Here, we summarize some of the ways in which the resulting mutant libraries in particular genes have been used for the analysis of protein structure-function relationships and in engineering applications.
authors.affiliation: Department of Microbiology, University of Washington, Seattle, WA, USA.
authors.forename: Beth
authors.initials: B
authors.lastname: Traxler
authors.forename: Eliora
authors.initials: E
authors.lastname: Gachelet
mesh_terms: D004251:DNA Transposable Elements; D020224:Expressed Sequence Tags; D015723:Gene Library; D005798:Genes, Bacterial; D005818:Genetic Engineering; D009154:Mutation; D013329:Structure-Activity Relationship
chemical_list: D004251:DNA Transposable Elements