title: Vaccine potential of novel surface exposed and secreted proteins of Streptococcus equi.
abstract: Streptococcus equi, a clonal descendent of an ancestral S. zooepidemicus, causes equine strangles, a highly contagious purulent lymphadenitis of the head and neck. The aim of this study was to evaluate as vaccine components novel surface exposed or secreted S. equi proteins identified in an expression gene library with sera from resistant horses. Six proteins expressed by S. equi CF32 but not by S. zooepidemicus 631 were used to vaccinate one group of eight ponies. A second pony group was immunized with five adhesin and other proteins encoded by genes of Linkage Gr 1. All ponies made strong serum antibody responses to each protein as measured by ELISA but none were resistant to subsequent comingling challenge with S. equi CF32. These results in combination with evidence that recovered horses rapidly clear intranasally inoculated S. equi and do not make detectable serum antibody responses to its surface proteins suggest that acquired immune-mediated tonsillar clearance and not serum antibody must be stimulated by an effective strangles vaccine.
authors.affiliation: Gluck Equine Research Center, University of Kentucky, Lexington, KY 40546, United States. jtimoney@uky.edu
authors.forename: John F
authors.initials: JF
authors.lastname: Timoney
authors.forename: Aiping
authors.initials: A
authors.lastname: Qin
authors.forename: Suresh
authors.initials: S
authors.lastname: Muthupalani
authors.forename: Sergey
authors.initials: S
authors.lastname: Artiushin
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D006734:Horse Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D006736:Horses; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D013290:Streptococcal Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D022541:Streptococcal Vaccines / Q000276:immunology*; D018502:Streptococcus equi / Q000235:genetics / Q000276:immunology*; D022223:Vaccines, Subunit / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D008565:Membrane Proteins; D022541:Streptococcal Vaccines; D022223:Vaccines, Subunit