HTLV-I alters the multidrug resistance associated protein 1 (ABCC1/MRP1) expression and activity in human T cells.
The human T cell lymphotropic/leukaemia virus type I (HTLV-I) causes HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP). The multidrug resistance associated protein 1 (ABCC1) plays multiple functions in physiopathologic responses. The expression and activity of ABCC1 was studied in T lymphocytes from uninfected and HTLV-I-infected individuals (both asymptomatic and symptomatic/HAM/TSP). ABCC1 expression and activity was reduced to nearly half in T lymphocytes from infected patients compared to control lymphocytes. Only 51.6% of CD4(+) cells from HAM/TSP patients expressed ABCC1 whereas this was seen in 60.3% from asymptomatic individuals, compared to an expression of around 86% in controls. Our results suggest that ABCC1 is negatively regulated in HTVL-I infection, supplying a novel target to investigate the pathogenesis of HTLV-I.
Echevarria-Lima
Juliana
J
Instituto de Pesquisa Clínica Evandro Chagas (IPEC), Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil.
Rumjanek
Vivian M
VM
Kyle-Cezar
Fernanda
F
Harab
Ramza C
RC
Leite
Ana Claudia Celestino B
AC
dos Santos Ornellas
Débora
D
Moralles
Marcelo Marcos
MM
Araújo
Abelardo Q C
AQ
Andrada-Serpa
Maria José
MJ
D000328:Adult; D000368:Aged; D015153:Blotting, Western; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism / Q000821:virology*; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism / Q000821:virology; D005260:Female; D005434:Flow Cytometry; D015490:HTLV-I Infections / Q000378:metabolism*; D015368:Human T-lymphotropic virus 1; D006801:Humans; D008297:Male; D008875:Middle Aged; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000096:biosynthesis*; D015493:Paraparesis, Tropical Spastic / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D027425:ATP-Binding Cassette, Sub-Family C Proteins; C421109:multidrug resistance-associated protein 1