Effects of BPA and DES on longchin goby (Chasmichthys dolichognathus) in vitro during the oocyte maturation.
This study was performed in order to assess whether bisphenol (BPA) and diethylstilbestrol (DES) had agonistic or antagonistic effects on oocyte maturation using marine fish. We tested the effects of these chemicals on in vitro maturation, germinal vesicle breakdown (GVBD), assay using oocytes from the longchin goby, Chasmichthys dolichognathus. During the maturation process, low concentrations of BPA and DES triggered GVBD depending on the stage of oocyte development; BPA at 0.044 nM and DES at 0.037, 0.37, and 3.73 nM induced GVBD in 0.82-0.88 mm diameter oocytes (germinal vesicle located near the center of oocytes). In 0.76-0.80 mm diameter oocytes (fully vitellogenic oocytes), BPA induced GVBD at relatively higher concentrations (4.38, 43.8, and 438 nM). In 0.86-0.90 mm diameter oocytes, BPA and DES had no observable effect on GVBD at the concentrations tested. Oocytes with diameters between 0.82 and 0.88 mm appeared to be more sensitive to these chemicals. Moreover, our results showed that BPA and DES did not inhibit GVBD.
Baek
Hea Ja
HJ
Department of Marine Biology, Pukyong National University, Busan 608-737, Republic of Korea. hjbaek@pknu.ac.kr
Hwang
In Joon
IJ
Kim
Kyung Sun
KS
Lee
Young Don
YD
Kim
Hyung Bae
HB
Yoo
Myong Suk
MS
D000818:Animals; D001559:Benzhydryl Compounds; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004054:Diethylstilbestrol / Q000633:toxicity*; D005260:Female; D009865:Oocytes / Q000187:drug effects*; D010473:Perciformes / Q000502:physiology*; D010636:Phenols / Q000633:toxicity*; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D006063:Chorionic Gonadotropin; D004054:Diethylstilbestrol; D010636:Phenols; D014874:Water Pollutants, Chemical; C006780:bisphenol A; D000098826:Bisphenol A Compounds