title: Synergetic effects of nanoporous support and urea on enzyme activity.
abstract: We report synergetic effects of functionalized mesoporous silica (FMS) and urea to promote favorable protein conformational changes. The specific activity of glucose isomerase (GI) entrapped in FMS in the presence of urea was approximately double that of GI in solution in the absence of urea. Rather than losing all activity in a denaturing solution of 8.0 M urea, the specific activity of GI entrapped in FMS remained higher than the highest specific activity of GI free in solution.
authors.affiliation: Pacific Northwest National Laboratory, Richland, Washington 99352, USA.
authors.forename: Chenghong
authors.initials: C
authors.lastname: Lei
authors.forename: Yongsoon
authors.initials: Y
authors.lastname: Shin
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Ackerman
mesh_terms: D000327:Adsorption; D019747:Aldose-Ketose Isomerases / Q000737:chemistry*; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000737:chemistry; D008422:Materials Testing; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D016062:Porosity; D011485:Protein Binding; D012822:Silicon Dioxide / Q000737:chemistry*; D014508:Urea / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D012822:Silicon Dioxide; D014508:Urea; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase