title: Steroid 9alpha-hydroxylation during testosterone degradation by resting rhodococcus equi cells.
abstract: The conversion pathway of testosterone to androst-4-ene-3,17-dione and 9alpha-hydroxy androstane metabolites, 9alpha-hydroxyandrost-4-ene-3,17-dione and 9alpha,17beta-dihydroxyandrost-4-en-3-one was proposed for the ring degradation in steroids by a minimal liquid medium (NMMP)-dispersed Rhodococcus equi ATCC 14887. The microorganism produced 9alpha-hydroxy androstane metabolites from testosterone at high conversion ratio without the addition of ring degradation inhibitory agents. Several NMMP-based media showed the similar effect on the microbial transformation, in which the respective molar yields of 9alpha-hydroxyandrost-4-ene-3,17-dione and 9alpha,17beta-dihydroxyandrost-4-en-3-one were approx. 3 to 47% and approx. 3 to 11%, respectively, whereas nutrient broth, a rich medium, basically showed no accumulation. On the basis of this evidence, magnesium sulfate and casamino acids among the components of NMMP were found to compromise the determinant for the production of the 9alpha-hydroxy androstane metabolites without appreciable decomposition of the steroid ring system.
authors.affiliation: Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
authors.forename: Yong-ung
authors.initials: YU
authors.lastname: Kim
authors.forename: Jaehong
authors.initials: J
authors.lastname: Han
authors.forename: Sang Sup
authors.initials: SS
authors.lastname: Lee
authors.forename: Kuniyoshi
authors.initials: K
authors.lastname: Shimizu
authors.forename: Yuji
authors.initials: Y
authors.lastname: Tsutsumi
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Kondo
mesh_terms: D000731:Androstanes / Q000378:metabolism; D001711:Biotransformation; D003470:Culture Media; D006900:Hydroxylation; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D016929:Rhodococcus equi / Q000378:metabolism*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013250:Steroid Hydroxylases / Q000378:metabolism*; D013739:Testosterone / Q000378:metabolism*
chemical_list: D000731:Androstanes; D003470:Culture Media; D013739:Testosterone; D013250:Steroid Hydroxylases; C036815:steroid 9-alpha-hydroxylase; C033936:androstane