Simultaneous identification of Bulbus Fritillariae cirrhosae using PCR-RFLP analysis.
Bulbus Fritillariae (BF) is the most commonly used antitussive herb in China. There are nine species of Fritillaria recorded as the drug BF in the Chinese Pharmacopoeia. Bulbus Fritillariae cirrhosae (BF cirrhosae) is a group that includes four species of BF; these four species come from wild sources with higher efficiency and lower toxicity compared to the other five species of BF. Due to reasons of carelessness and reduced costs, the other five species are often sold as BF cirrhosae. Analysis through appearance, microscopic and chemical techniques has limitations. Identifying botanical resources is a primary step in the standardization of herbal medicine. In the present article, the internal transcribed spacer 1 (ITS1) regions of the nuclear ribosomal DNA (nrDNA) of nine species and one variety of Fritillaria genus have been sequenced. A mutation site in the ITS1 region among BF cirrhosae and other species of BF has been found and can be recognized by the restriction endonuclease SmaI. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the nuclear ribosomal ITS1 region was used to differentiate BF cirrhosae from other species of BF and is a successful method in distinguishing the subgroups.
Wang
C Z
CZ
Key Laboratory of Modern Chinese Medicines, and Department of Pharmacognosy, School of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing, JS 210038, China.
Li
P
P
Ding
J Y
JY
Peng
X
X
Yuan
C S
CS
D000996:Antitussive Agents / Q000737:chemistry*; D001483:Base Sequence; D017931:DNA Primers; D018744:DNA, Plant / Q000032:analysis; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D031410:Fritillaria / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D008517:Phytotherapy; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D000996:Antitussive Agents; D017931:DNA Primers; D018744:DNA, Plant; D004365:Drugs, Chinese Herbal