title: Immunolocalization of insulin-like growth factor I (IGF-I) during preimplantation in rat uterus.
abstract: OBJECTIVE
  The aim of this study was to determine the insulin-like growth factor (IGF-I) immunolocalization in rat uterus at preimplantation period.
  
  
  METHODS
  The tissue samples were examined from pregnant animals between gestational day 1 and 5 using immunocytochemistry.
  
  
  RESULTS
  While both the uterine luminal epithelium and the glandular epithelium were stained strongly from gestational day 1 - 3 by IGF-I antibody, the IGF-I immunoreactivity was moderate in myometrium and capillary endothelium. At this period, the IGF-I immunoreactivity was weakly present in a few endometrial stromal cells. At day 4, while IGF-I immunostaining intensity was particularly decreased in the basal domains of uterine luminal epithelium and glandular epithelium, it was similar to the first 3 days of gestation in myometrium and capillary endothelium. The endometrial vascularization and the number of stromal cells immunoreacting with IGF-I increased. The differentiation of stromal cells into decidual cells was seen at gestational day 5 and IGF-I was strongly expressed in the decidualizing cells. The IGF-I immunoreactivity in uterine luminal epithelium and glandular epithelium was similar to gestational day 4. IGF-I immunoreactivity was strongly detected in all of endometrial stromal cells.
  
  
  CONCLUSIONS
  These results indicate that IGF-I expression in the rat uterus changes in the early pregnancy process and increase toward to day when implantation will be initiated.
authors.affiliation: Department of Histology and Embryology, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, 15100 Burdur, Turkey. jaleoner@hotmail.com
authors.forename: Jale
authors.initials: J
authors.lastname: Oner
authors.forename: Hakan
authors.initials: H
authors.lastname: Oner
mesh_terms: D000818:Animals; D010064:Embryo Implantation; D030762:Estrous Cycle / Q000378:metabolism; D005260:Female; D007150:Immunohistochemistry; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D011247:Pregnancy; D011270:Pregnancy, Animal; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution; D014599:Uterus / Q000378:metabolism*
chemical_list: D007334:Insulin-Like Growth Factor I