Influence of novel resin monomer on viability of L-929 mouse fibroblasts in vitro.
We have previously synthesized a novel acrylic resin monomer, methacryloyloxyethyl methyl succinate (TA). The aim of this in vitro study, therefore, was to examine its influence on cell viability using L-929 mouse fibroblasts and then compare the results with MMA, EMA, and LMA. Medium containing each monomer was changed every 15 minutes as some monomers were volatile. After one hour of exposure, these mediums were replaced with a normal medium and cells were further incubated for 72 hours. IC50 value for each monomer was determined, and chronological cell viability and cytomorphologic observation were evaluated. Viability was impaired in a dose-dependent manner. All monomers, except TA, tended to correlate between molecular weight and cell viability. On the other hand, TA showed excellent viability and did not impair growth abruptly. These results thus demonstrated that cellular damage by TA was much lower than that by other monomers.
Jinno
Satoshi
S
Department of Periodontology, School of Dentistry, Aichi-Gakuin University, 2-11 Suemori-dori, Chikusa-ku, Nagoya 464-8651, Japan. sjin@sdent.aichi-gakuin.ac.jp
Kawai
Tatsushi
T
Ishikawa
Atsuko
A
Suzuki
Tomoo
T
Hattori
Nobuaki
N
Okeya
Hiroyuki
H
Hayashi
Tatsuhide
T
Maeda
Hatsuhiko
H
Ohno
Yuzo
Y
Ito
Masamitsu
M
Noguchi
Toshihide
T
D000180:Acrylic Resins / Q000737:chemistry* / Q000633:toxicity*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects*; D020128:Inhibitory Concentration 50; D007739:L Cells; D051379:Mice; D008970:Molecular Weight; D013997:Time Factors; D014835:Volatilization
D000180:Acrylic Resins