On-line liquid chromatography-electrospray ionisation mass spectrometry for kappa-carrageenan oligosaccharides with a porous graphitic carbon column.
Enzymatically digested oligosaccharides of kappa-carrageenans were separated on a porous graphitic carbon (PGC) column and characterised on-line by electrospray ionisation mass spectrometry (ESI-MS). Two different developing ions were applied. Among them ammonium hydrogencarbonate showed more eluting power as it should on normal anion-exchange stationary phases. The oligosaccharides were detected by ESI-MS as fully deprotonated oligosaccharides.
Antonopoulos
Aristotelis
A
Institut de Chimie Organique et Analytique (ICOA), UMR CNRS 6005, Université d'Orléans, B.P. 6759, 45067 Orléans Cedex 2, France.
Favetta
Patrick
P
Helbert
William
W
Lafosse
Michel
M
D000837:Anion Exchange Resins; D002244:Carbon / Q000737:chemistry*; D002351:Carrageenan / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D006108:Graphite / Q000737:chemistry*; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry; D016062:Porosity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D000837:Anion Exchange Resins; D009844:Oligosaccharides; D002244:Carbon; D006108:Graphite; D002351:Carrageenan