title: Large-scale mapping of human protein-protein interactions by mass spectrometry.
abstract: Mapping protein-protein interactions is an invaluable tool for understanding protein function. Here, we report the first large-scale study of protein-protein interactions in human cells using a mass spectrometry-based approach. The study maps protein interactions for 338 bait proteins that were selected based on known or suspected disease and functional associations. Large-scale immunoprecipitation of Flag-tagged versions of these proteins followed by LC-ESI-MS/MS analysis resulted in the identification of 24,540 potential protein interactions. False positives and redundant hits were filtered out using empirical criteria and a calculated interaction confidence score, producing a data set of 6463 interactions between 2235 distinct proteins. This data set was further cross-validated using previously published and predicted human protein interactions. In-depth mining of the data set shows that it represents a valuable source of novel protein-protein interactions with relevance to human diseases. In addition, via our preliminary analysis, we report many novel protein interactions and pathway associations.
authors.affiliation: Protana, Toronto, Ontario, Canada.
authors.forename: Rob M
authors.initials: RM
authors.lastname: Ewing
authors.forename: Peter
authors.initials: P
authors.lastname: Chu
authors.forename: Fred
authors.initials: F
authors.lastname: Elisma
authors.forename: Hongyan
authors.initials: H
authors.lastname: Li
authors.forename: Paul
authors.initials: P
authors.lastname: Taylor
authors.forename: Shane
authors.initials: S
authors.lastname: Climie
authors.forename: Linda
authors.initials: L
authors.lastname: McBroom-Cerajewski
authors.forename: Mark D
authors.initials: MD
authors.lastname: Robinson
authors.forename: Liam
authors.initials: L
authors.lastname: O'Connor
authors.forename: Michael
authors.initials: M
authors.lastname: Li
authors.forename: Rod
authors.initials: R
authors.lastname: Taylor
authors.forename: Moyez
authors.initials: M
authors.lastname: Dharsee
authors.forename: Yuen
authors.initials: Y
authors.lastname: Ho
authors.forename: Adrian
authors.initials: A
authors.lastname: Heilbut
authors.forename: Lynda
authors.initials: L
authors.lastname: Moore
authors.forename: Shudong
authors.initials: S
authors.lastname: Zhang
authors.forename: Olga
authors.initials: O
authors.lastname: Ornatsky
authors.forename: Yury V
authors.initials: YV
authors.lastname: Bukhman
authors.forename: Martin
authors.initials: M
authors.lastname: Ethier
authors.forename: Yinglun
authors.initials: Y
authors.lastname: Sheng
authors.forename: Julian
authors.initials: J
authors.lastname: Vasilescu
authors.forename: Mohamed
authors.initials: M
authors.lastname: Abu-Farha
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Lambert
authors.forename: Henry S
authors.initials: HS
authors.lastname: Duewel
authors.forename: Ian I
authors.initials: II
authors.lastname: Stewart
authors.forename: Bonnie
authors.initials: B
authors.lastname: Kuehl
authors.forename: Kelly
authors.initials: K
authors.lastname: Hogue
authors.forename: Karen
authors.initials: K
authors.lastname: Colwill
authors.forename: Katharine
authors.initials: K
authors.lastname: Gladwish
authors.forename: Brenda
authors.initials: B
authors.lastname: Muskat
authors.forename: Robert
authors.initials: R
authors.lastname: Kinach
authors.forename: Sally-Lin
authors.initials: SL
authors.lastname: Adams
authors.forename: Michael F
authors.initials: MF
authors.lastname: Moran
authors.forename: Gregg B
authors.initials: GB
authors.lastname: Morin
authors.forename: Thodoros
authors.initials: T
authors.lastname: Topaloglou
authors.forename: Daniel
authors.initials: D
authors.lastname: Figeys
mesh_terms: D006801:Humans; D047468:Immunoprecipitation; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D011506:Proteins