Characterisation of cyclin D1 down-regulation in coronavirus infected cells.
The positive strand RNA coronavirus, infectious bronchitis virus (IBV), induces a G2/M phase arrest and reduction in the G1 and G1/S phase transition regulator cyclin D1. Quantitative real-time RT-PCR and Western blot analysis demonstrated that cyclin D1 was reduced post-transcriptionally within infected cells independently of the cell-cycle stage at the time of infection. Confocal microscopy revealed that cyclin D1 decreased in IBV-infected cells as infection progressed and inhibition studies indicated that a population of cyclin D1 could be targeted for degradation by a virus mediated pathway. In contrast to the SARS-coronavirus, IBV nucleocapsid protein did not interact with cyclin D1.
Harrison
Sally M
SM
Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK.
Dove
Brian K
BK
Rothwell
Lisa
L
Kaiser
Pete
P
Tarpey
Ian
I
Brooks
Gavin
G
Hiscox
Julian A
JA
D000818:Animals; D015153:Blotting, Western; D002522:Chlorocebus aethiops; D017934:Coronavirus; D019938:Cyclin D1 / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D001351:Infectious bronchitis virus; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D012367:RNA, Viral / Q000032:analysis / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014709:Vero Cells
D012333:RNA, Messenger; D012367:RNA, Viral; D019938:Cyclin D1