title: Expression and localization of CKLFSF2 in human spermatogenesis.
abstract: OBJECTIVE
  To investigate the expression and subcellular localization of chemokine-like factor superfamily 2 (CKLFSF2) in human testis and its potential role in spermatogenesis.
  
  
  METHODS
  A specific polyclonal antibody against CKLFSF2 was raised. The expression and cellular localization of CKLFSF2 in the seminiferous tubules was checked by immunohistochemistry method. Also, in situ hybridization was applied to localize the mRNA distribution. The EGFP-CKLFSF2 fusion protein was expressed in COS-7 cells to localize its subcellular location in vitro. In addition, the abnormal expression of CKLFSF2 in testes of patients with male infertility was assayed by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry methods.
  
  
  RESULTS
  Having a close correlation with spermatogenesis defects, CKLFSF2 was specifically expressed in meiotic and post-meiotic germ cells, which were localized to the endoplasmic reticulum (ER) near the Golgi apparatus.
  
  
  CONCLUSIONS
  CKLFSF2 could play important roles in the process of meiosis and spermiogenesis, and might be involved in the vesicular transport or membrane apposition events in the endoplasmic reticulum.
authors.affiliation: Andrology Center, Peking University First Hospital, Beijing 100009, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.forename: Zhong-Cheng
authors.initials: ZC
authors.lastname: Xin
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.forename: Long
authors.initials: L
authors.lastname: Tian
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Yuan
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Song
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Jiang
authors.forename: Ying-Lu
authors.initials: YL
authors.lastname: Guo
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D019556:COS Cells; D018925:Chemokines / Q000096:biosynthesis* / Q000276:immunology; D002522:Chlorocebus aethiops; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005854:Germ Cells / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007248:Infertility, Male / Q000378:metabolism; D062792:MARVEL Domain-Containing Proteins; D008297:Male; D008540:Meiosis; D018613:Microscopy, Confocal; D013091:Spermatogenesis / Q000502:physiology*; D013737:Testis / Q000378:metabolism
chemical_list: C505071:CMTM2 protein, human; D018925:Chemokines; D062792:MARVEL Domain-Containing Proteins