Studies on the uptake of glucose derivatives by red blood cells.
Erythrocytes express the same glucose transporter (GLUT-1) as is present in the blood-brain barrier. With the aim of testing the viability of using this transport system to deliver glucosyl drug derivatives to the brain, the uptake of several dopamine-glucose conjugates and a few structurally related analogues by erythrocytes was studied with HPLC and (1)H MAS NMR spectroscopy. The results showed that slight structural changes determine the uptake of glycoconjugates by red blood cells. However, experiments in the presence of glucose transport inhibitors showed that none of the conjugates that efficiently crossed the cell membrane were transported by GLUT-1.
García-Alvarez
Isabel
I
Instituto de Química Orgánica General, CSIC, Juan de la Cierva 3, 28006 Madrid, Spain.
Garrido
Leoncio
L
Fernández-Mayoralas
Alfonso
A
D004298:Dopamine / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D015195:Drug Design; D004912:Erythrocytes / Q000378:metabolism*; D005947:Glucose / Q000031:analogs & derivatives* / Q000378:metabolism*; D051272:Glucose Transporter Type 1 / Q000378:metabolism; D006801:Humans; D013329:Structure-Activity Relationship
D051272:Glucose Transporter Type 1; C495677:SLC2A1 protein, human; D005947:Glucose; D004298:Dopamine