title: Olive variety identification by ligation detection reaction in a universal array format.
abstract: Molecular methodologies are increasingly being developed to assess the origin and authenticity of raw organic materials and processed food products. Here we describe the application of a microarray-based assay for single nucleotide polymorphisms (SNPs) identification in olive cultivars. The assay distinguishes alleles in a ligation detection reaction (LDR), with subsequent fluorescent detection by hybridization on a universal array (UA). The LDR-UA approach was used to detect 17 SNPs in olive genomic sequences previously amplified by PCR from fresh olive leaves. Genotype calls obtained with the LDR-UA were in full agreement with those determined by direct sequencing. The panel of 17 SNPs is sufficient to discriminate 49 olive varieties selected among the most widely cultivated for olive oil production in the Mediterranean area.
authors.affiliation: Institute of Biomedical Technologies, National Research Council, LITA, Via Fratelli Cervi 93, I-20090 Segrate (MI), Italy. clarissa.consolandi@itb.cnr.it
authors.forename: Clarissa
authors.initials: C
authors.lastname: Consolandi
authors.forename: Luisa
authors.initials: L
authors.lastname: Palmieri
authors.forename: Silvia
authors.initials: S
authors.lastname: Doveri
authors.forename: Elena
authors.initials: E
authors.lastname: Maestri
authors.forename: Nelson
authors.initials: N
authors.lastname: Marmiroli
authors.forename: Silvia
authors.initials: S
authors.lastname: Reale
authors.forename: David
authors.initials: D
authors.lastname: Lee
authors.forename: Luciana
authors.initials: L
authors.lastname: Baldoni
authors.forename: Nicola
authors.initials: N
authors.lastname: Tosti
authors.forename: Marco
authors.initials: M
authors.lastname: Severgnini
authors.forename: Gianluca
authors.initials: G
authors.lastname: De Bellis
authors.forename: Bianca
authors.initials: B
authors.lastname: Castiglioni
mesh_terms: D016000:Cluster Analysis; D017931:DNA Primers; D005453:Fluorescence; D005838:Genotype; D046228:Microarray Analysis / Q000379:methods*; D031658:Olea / Q000235:genetics*; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D013045:Species Specificity
chemical_list: D017931:DNA Primers