A silicone-based controlled-release device for accelerated proteolytic debridement of wounds.
A new device for rapid enzymatic debridement of cutaneous wounds has been developed using a controlled-release, silicone-based, dried emulsion. A dehydrated serine protease of the subtilisin family, previously untested for wound debridement, was incorporated into the emulsion. This device exhibited excellent storage stability. Moisture from the wound triggered an even, reproducible, and complete release of the enzyme within the first 8 hours. The device maintains a moist wound environment that allows the enzyme to achieve nearly complete digestion of the hardened eschar of full-thickness burns in a porcine model after an exposure period of 24 hours. Debridement was faster than in untreated wounds or wounds treated with a currently available enzyme ointment. Following rapid enzymatic debridement, healing appeared to progress normally, with no histological evidence of damage to adjacent healthy tissue.
Bott
Richard
R
Danisco, Palo Alto, California, USA. rick.bott@danisco.com
Crissman
James
J
Kollar
Csilla
C
Saldajeno
Mae
M
Ganshaw
Grant
G
Thomas
Xavier
X
Lane
Thomas H
TH
Klykken
Paal
P
Davidson
Jeffrey M
JM
Nanney
Lillian B
LB
D000818:Animals; D002056:Burns / Q000601:surgery*; D003646:Debridement / Q000295:instrumentation* / Q000379:methods; D003879:Dermatologic Agents / Q000008:administration & dosage*; D004195:Disease Models, Animal; D016503:Drug Delivery Systems; D004655:Emulsions; D009779:Occlusive Dressings; D009824:Ointments; D010206:Papain / Q000008:administration & dosage; D010865:Pilot Projects; D012828:Silicones; D020860:Subtilisin / Q000008:administration & dosage*; D013552:Swine
D003879:Dermatologic Agents; D004655:Emulsions; D009824:Ointments; D012828:Silicones; D020860:Subtilisin; D010206:Papain