Oct4 and Sox2 directly regulate expression of another pluripotency transcription factor, Zfp206, in embryonic stem cells.
It is well known that Oct4 and Sox2 play an important role in the maintenance of embryonic stem cell pluripotency. These transcription factors bind to regulatory regions within hundreds of target genes to control their expression. Zfp206 is a recently characterized transcription factor that has a role in maintaining stem cell pluripotency. We have demonstrated here that Zfp206 is a direct downstream target of Oct4 and Sox2. Two composite sox-oct binding sites have been identified within the first intron of Zfp206. We have demonstrated binding of Oct4 and Sox2 to this region. In addition, we have shown that Oct4 or Sox2 alone can activate transcription via one of these sox-oct elements, although the presence of both Oct4 and Sox2 gave rise to a synergistic effect. These studies extend our understanding of the transcriptional network that operates to regulate the differentiation potential of embryonic stem cells.
Wang
Zheng-Xu
ZX
Department of Stem Cell and Developmental Biology, Genome Institute of Singapore, Singapore 138672.
Teh
Christina Hui-Leng
CH
Kueh
Jacqueline L L
JL
Lufkin
Thomas
T
Robson
Paul
P
Stanton
Lawrence W
LW
D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D051379:Mice; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism*; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D020218:Response Elements / Q000502:physiology; D055748:SOXB1 Transcription Factors; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic / Q000502:physiology
D004268:DNA-Binding Proteins; D050814:Octamer Transcription Factor-3; D055748:SOXB1 Transcription Factors; D015534:Trans-Activators; D014157:Transcription Factors; C494174:Pou5f1 protein, mouse; C527783:Sox2 protein, mouse; C514588:Zscan10 protein, mouse