[Establishment and analysis of serum two-dimensional gel electrophoresis profiles of myasthenia gravis patients with spleen and kidney deficiency syndrome].
OBJECTIVE
The purpose of this study was to establish two-dimensional gel electrophoresis (2-DE) profiles of serum of myasthenia gravis patients, and to identify the differential proteomic expressions between normal persons and myasthenia gravis patients with spleen and kidney deficiency syndrome.


METHODS
Samples of serum protein were extracted by repeated freeze-thaw method and separated by two-dimensional electrophoresis. Differential proteomic expressions between the myasthenia gravis patients and the normal control persons were identified by two-dimensional polyacrylamide gel electrophoresis, silver staining, image-master 2-DE software analysis, peptide mass fingerprinting based on matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS), BioWorks and NCBI software database searching.


RESULTS
The two-dimensional polyacrylamide gel electrophoresis profiles of serum proteins were successfully established by 2-DE. Twenty-one of the significant differential proteins were selected and identified by MALDI-TOF-MS. Eight of them were finally identified.


CONCLUSIONS
The 2-DE profiles of serum proteins were established and the differential proteomic expressions were identified by proteome technique in our study. This can be an experimental basis for further research of the pathogenesis and treatment of myasthenia gravis.
Liu
Ping
P
Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China. Liuping23@sina.com
Zhang
Yi-yi
YY
Qiao
Jian
J
D001797:Blood Protein Electrophoresis / Q000379:methods*; D001798:Blood Proteins / Q000032:analysis*; D003937:Diagnosis, Differential; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008516:Medicine, Chinese Traditional; D009157:Myasthenia Gravis / Q000097:blood* / Q000175:diagnosis; D010449:Peptide Mapping / Q000379:methods; D040901:Proteomics / Q000379:methods; D013577:Syndrome; D016711:Yang Deficiency / Q000097:blood / Q000175:diagnosis
D001798:Blood Proteins