Production and characterization of monoclonal antibodies sensitive to conformation in the 5HT2c serotonin receptor.
mAbs that are sensitive to protein conformation can be helpful in studies of protein structure and function; in particular, mAb fragments are useful reagents in membrane protein crystallization. We immunized mice with the rat 5HT2c serotonin receptor and derived clonal hybridoma cells, which we tested for specific antigen reactivity by using the complementarity of purified protein from bacteria and receptor-embedded mammalian cell membranes. Nine mAbs met our criteria for specificity, affinity, and sensitivity to conformational features. Epitopes were mapped in various additional tests. Five of the nine mAbs have cytoplasmic epitopes, and two of these are sensitive to the ligand state of the receptor. These properties should be useful both for structural analysis and in probes of function.
Mancia
Filippo
F
Department of Biochemistry and Molecular Biophysics and the Howard Hughes Medical Institute, Columbia University, New York, NY 10032, USA.
Brenner-Morton
Susan
S
Siegel
Risa
R
Assur
Zahra
Z
Sun
Yonghua
Y
Schieren
Ira
I
Mendelsohn
Monica
M
Axel
Richard
R
Hendrickson
Wayne A
WA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D002462:Cell Membrane / Q000378:metabolism; D002469:Cell Separation; D003460:Crystallization; D003593:Cytoplasm / Q000378:metabolism; D018604:Epitope Mapping; D004926:Escherichia coli / Q000378:metabolism; D006825:Hybridomas / Q000378:metabolism; D008024:Ligands; D051379:Mice; D041681:NIH 3T3 Cells; D011487:Protein Conformation; D051381:Rats; D011985:Receptors, Serotonin / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D008024:Ligands; D011985:Receptors, Serotonin