Molecular detection of Chlamydia trachomatis and HPV infections in cervical samples with normal and abnormal cytopathological findings.
It has been suggested that Chlamydia trachomatis (CT) and human papillomaviruses (HPV) co-infection could contribute to development of intraepithelial lesions. In this study, HPV and CT-DNA were investigated in 250 cervicovaginal samples of patients from Minas Gerais, Brazil. The cytological analysis revealed that 70% of samples (175) were negative, 5.2% (13) presented atypical squamous or glandular cells of undetermined significance (ASCUS/AGUS), 12.4% (31) presented low-grade squamous intraepithelial lesion (LSIL), 10.8% (27) high-grade squamous intraepithelial lesion (HSIL), and 1.6% (4) invasive carcinoma. HPV-DNA and HPV/CT co-infection was observed in 40% (100/250) and in 5.2% (13/250) of samples, respectively. Among the positive cytological samples, HPV-DNA was detected in 73.3% and CT-DNA in 9.33% and in 13%, if only the HPV positive samples were considered. The highest co-infection rate (15.4%) was observed among ASCUS/AGUS samples. Although a significant association was found for HPV infection and the precursor lesions of cervical cancer, it was not possible to establish a significant association between these lesions and CT or HPV/CT co-infection.
de Paula
Francisco Danilo Ferreira
FD
Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, CEP 31270 901 Belo Horizonte, Minas Gerais, Brazil.
Fernandes
Ana Paula
AP
Carmo
Bianca Bianco do
BB
Vieira
Daniela Cristina Duarte
DC
Dutra
Miriam Santos
MS
Santos
Caleb Guedes Miranda dos
CG
Souza
Maria do Carmo M
Mdo C
Andrade
Túlio César Alvim
TC
Vago
Annamaria Ravara
AR
Fernandes
Paula Avila
PA
D000328:Adult; D002584:Cervix Uteri / Q000473:pathology* / Q000821:virology*; D002690:Chlamydia Infections / Q000150:complications* / Q000453:epidemiology; D002692:Chlamydia trachomatis / Q000302:isolation & purification; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000150:complications* / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D011230:Precancerous Conditions / Q000821:virology; D014412:Tumor Virus Infections / Q000150:complications / Q000453:epidemiology*; D002583:Uterine Cervical Neoplasms / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000821:virology
D004279:DNA, Viral