title: HTLV-I alters the multidrug resistance associated protein 1 (ABCC1/MRP1) expression and activity in human T cells.
abstract: The human T cell lymphotropic/leukaemia virus type I (HTLV-I) causes HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP). The multidrug resistance associated protein 1 (ABCC1) plays multiple functions in physiopathologic responses. The expression and activity of ABCC1 was studied in T lymphocytes from uninfected and HTLV-I-infected individuals (both asymptomatic and symptomatic/HAM/TSP). ABCC1 expression and activity was reduced to nearly half in T lymphocytes from infected patients compared to control lymphocytes. Only 51.6% of CD4(+) cells from HAM/TSP patients expressed ABCC1 whereas this was seen in 60.3% from asymptomatic individuals, compared to an expression of around 86% in controls. Our results suggest that ABCC1 is negatively regulated in HTVL-I infection, supplying a novel target to investigate the pathogenesis of HTLV-I.
authors.affiliation: Instituto de Pesquisa Clínica Evandro Chagas (IPEC), Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil.
authors.forename: Juliana
authors.initials: J
authors.lastname: Echevarria-Lima
authors.forename: Vivian M
authors.initials: VM
authors.lastname: Rumjanek
authors.forename: Fernanda
authors.initials: F
authors.lastname: Kyle-Cezar
authors.forename: Ramza C
authors.initials: RC
authors.lastname: Harab
authors.forename: Ana Claudia Celestino B
authors.initials: AC
authors.lastname: Leite
authors.forename: Débora
authors.initials: D
authors.lastname: dos Santos Ornellas
authors.forename: Marcelo Marcos
authors.initials: MM
authors.lastname: Moralles
authors.forename: Abelardo Q C
authors.initials: AQ
authors.lastname: Araújo
authors.forename: Maria José
authors.initials: MJ
authors.lastname: Andrada-Serpa
mesh_terms: D000328:Adult; D000368:Aged; D015153:Blotting, Western; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism / Q000821:virology*; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism / Q000821:virology; D005260:Female; D005434:Flow Cytometry; D015490:HTLV-I Infections / Q000378:metabolism*; D015368:Human T-lymphotropic virus 1; D006801:Humans; D008297:Male; D008875:Middle Aged; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000096:biosynthesis*; D015493:Paraparesis, Tropical Spastic / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D027425:ATP-Binding Cassette, Sub-Family C Proteins; C421109:multidrug resistance-associated protein 1