[Expression and clinical value of eukaryotic initiation factor 4E and matrix metalloproteinase-9 in supraglottic and hypopharyngeal carcinoma and metastasis lymph].
OBJECTIVE
To study the expression of eukaryotic initiation factor4E (eIF4E)and matrix metalloproteinase-9 (MMP-9) in supraglottic, hypopharyngeal carcinoma, different distance surgical margins and lymphatic metastasis; to study the relationship of two molecular markers.


METHODS
Expression of elF4E and MMP-9 in carcinoma and different margins were detected by HE-stained, routine pathological section, immunohistochemical SP method and image analysis.


RESULTS
(1) Immunohistochemical staining for MMP-9 was performed with a monoclonal anti-MMP-9 antibody. The positive expression rate was 68. 4 % . There was significant difference between lymph node metastasis and no lymph node metastasis, but there was no significant difference between T3 and T4 or T1 and T2 ( P >0. 05). There was no significant difference between MMP-9 and carcinoma position,pathology grade ( P> 0. 05). Positive expression rate of MMP-9 was decreased in tumour , 5 mm, 10 mm different distant margin of supraglottic and hypopharyngeal carcinoma as tissue progressed adjacent to carcinoma. (2) Staining positive rate of elF4E in supraglottic and hypopharyngeal carcinoma was 89%. There was significant association between eIF4E expression intensity and clinical stage, lymphatic metastasis. The positive expression rate of eIF4E was increased in sequence from 10 mm, 5 mm distant mucosa tissue to carcinoma nest in supraglottic and hypopharyngeal carcinoma. (3) Negative expression of MMP-9 and eIF4E was seen in the normal lymph nodes. MMP-9 positive expression rate was 28% in metastasis lymph nodes,elF4E was 42 %. (4) There was no significant correlation between elF4E and MMP-9 in margins according to spearman rank correlation test.


CONCLUSIONS
eIF4E and MMP-9 expression was significantly higher in supraglottic and hypopharyngeal carcinoma than distant margin and normal mucosa tissue. elF4E positive rate was higher than MMP-9,expression sensitivity and reliability of elF4E in margins was higher than MMP-9. elF4E is a sensitive marker in growth, infiltration and metastasis of supraglottic and hypopharyngeal carcinoma.
Sun
Shujun
S
Department of Otolaryngology, Weifang Medical University Affiliated Hospital, Weifang, 261031, China. shujunsun720430@126.com
Liu
Qingming
Q
Zhang
Ailing
A
He
Enhai
E
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D039561:Eukaryotic Initiation Factor-4E / Q000378:metabolism*; D005260:Female; D006801:Humans; D007012:Hypopharyngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D007822:Laryngeal Neoplasms / Q000378:metabolism / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D008875:Middle Aged
D014408:Biomarkers, Tumor; D039561:Eukaryotic Initiation Factor-4E; D020780:Matrix Metalloproteinase 9