Immunomagnetic indirect positive sorting of precartilaginous stem cells from neonatal rat.
To investigate the technique of sorting high-purity precartilaginous stem cells from rat's perichondrium, neonatal rat's perichondrium cells suspensions were incubated with monoclone antibody of anti-fibroblast growth factor receptor-3 (anti-FGFR-3), and the labeled cells were separated from the suspension in the magnetic field by immuno-beads coated with the second antibody. Purity of the sorted neural stem cells was found to be 93.0%-99.0%, with living cells amounting to 80% -85 %. The magnetic cell sorting system could effectively separate precartilaginous stem cells from perichondrium cell suspensions.
Cheng
Hao
H
Department of Orthopedics, Tongji Hospital, Tongji Medical College of Huangzhong University of Science & Technology.
Chen
Anmin
A
You
Hongbo
H
D000818:Animals; D000831:Animals, Newborn; D002356:Cartilage / Q000166:cytology*; D002478:Cells, Cultured; D018189:Immunomagnetic Separation / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology*