title: Discovery of a natural thiamine adenine nucleotide.
abstract: Several important cofactors are adenine nucleotides with a vitamin as the catalytic moiety. Here, we report the discovery of the first adenine nucleotide containing vitamin B1: adenosine thiamine triphosphate (AThTP, 1), or thiaminylated ATP. We discovered AThTP in Escherichia coli and found that it accumulates specifically in response to carbon starvation, thereby acting as a signal rather than a cofactor. We detected smaller amounts in yeast and in plant and animal tissues.
authors.affiliation: Center for Cellular and Molecular Neurobiology, University of Liège, Avenue de l'Hôpital 1, B-4000 Liège, Belgium. l.bettendorff@ulg.ac.be
authors.forename: Lucien
authors.initials: L
authors.lastname: Bettendorff
authors.forename: Barbara
authors.initials: B
authors.lastname: Wirtzfeld
authors.forename: Alexander F
authors.initials: AF
authors.lastname: Makarchikov
authors.forename: Gabriel
authors.initials: G
authors.lastname: Mazzucchelli
authors.forename: Michel
authors.initials: M
authors.lastname: Frédérich
authors.forename: Tiziana
authors.initials: T
authors.lastname: Gigliobianco
authors.forename: Marjorie
authors.initials: M
authors.lastname: Gangolf
authors.forename: Edwin
authors.initials: E
authors.lastname: De Pauw
authors.forename: Luc
authors.initials: L
authors.lastname: Angenot
authors.forename: Pierre
authors.initials: P
authors.lastname: Wins
mesh_terms: D000255:Adenosine Triphosphate / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D008968:Molecular Conformation; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D013838:Thiamine Triphosphate / Q000302:isolation & purification*
chemical_list: C519454:adenosine thiamine triphosphate; D013838:Thiamine Triphosphate; D000255:Adenosine Triphosphate