title: Genome-wide functional analysis of pathogenicity genes in the rice blast fungus.
abstract: Rapid translation of genome sequences into meaningful biological information hinges on the integration of multiple experimental and informatics methods into a cohesive platform. Despite the explosion in the number of genome sequences available, such a platform does not exist for filamentous fungi. Here we present the development and application of a functional genomics and informatics platform for a model plant pathogenic fungus, Magnaporthe oryzae. In total, we produced 21,070 mutants through large-scale insertional mutagenesis using Agrobacterium tumefaciens-mediated transformation. We used a high-throughput phenotype screening pipeline to detect disruption of seven phenotypes encompassing the fungal life cycle and identified the mutated gene and the nature of mutation for each mutant. Comparative analysis of phenotypes and genotypes of the mutants uncovered 202 new pathogenicity loci. Our findings demonstrate the effectiveness of our platform and provide new insights on the molecular basis of fungal pathogenesis. Our approach promises comprehensive functional genomics in filamentous fungi and beyond.
authors.affiliation: Department of Agricultural Biotechnology, Center for Fungal Genetic Resources, and Center for Agricultural Biomaterials, Seoul National University, Seoul 151-921, Korea.
authors.forename: Junhyun
authors.initials: J
authors.lastname: Jeon
authors.forename: Sook-Young
authors.initials: SY
authors.lastname: Park
authors.forename: Myoung-Hwan
authors.initials: MH
authors.lastname: Chi
authors.forename: Jaehyuk
authors.initials: J
authors.lastname: Choi
authors.forename: Jongsun
authors.initials: J
authors.lastname: Park
authors.forename: Hee-Sool
authors.initials: HS
authors.lastname: Rho
authors.forename: Soonok
authors.initials: S
authors.lastname: Kim
authors.forename: Jaeduk
authors.initials: J
authors.lastname: Goh
authors.forename: Sungyong
authors.initials: S
authors.lastname: Yoo
authors.forename: Jinhee
authors.initials: J
authors.lastname: Choi
authors.forename: Ju-Young
authors.initials: JY
authors.lastname: Park
authors.forename: Mihwa
authors.initials: M
authors.lastname: Yi
authors.forename: Seonyoung
authors.initials: S
authors.lastname: Yang
authors.forename: Min-Jung
authors.initials: MJ
authors.lastname: Kwon
authors.forename: Seong-Sook
authors.initials: SS
authors.lastname: Han
authors.forename: Byeong Ryun
authors.initials: BR
authors.lastname: Kim
authors.forename: Chang Hyun
authors.initials: CH
authors.lastname: Khang
authors.forename: Bongsoo
authors.initials: B
authors.lastname: Park
authors.forename: Se-Eun
authors.initials: SE
authors.lastname: Lim
authors.forename: Kyongyong
authors.initials: K
authors.lastname: Jung
authors.forename: Sunghyung
authors.initials: S
authors.lastname: Kong
authors.forename: Maruthachalam
authors.initials: M
authors.lastname: Karunakaran
authors.forename: Hong-Sik
authors.initials: HS
authors.lastname: Oh
authors.forename: Hyojeong
authors.initials: H
authors.lastname: Kim
authors.forename: Seryun
authors.initials: S
authors.lastname: Kim
authors.forename: Jaejin
authors.initials: J
authors.lastname: Park
authors.forename: Soyoung
authors.initials: S
authors.lastname: Kang
authors.forename: Woo-Bong
authors.initials: WB
authors.lastname: Choi
authors.forename: Seogchan
authors.initials: S
authors.lastname: Kang
authors.forename: Yong-Hwan
authors.initials: YH
authors.lastname: Lee
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D005800:Genes, Fungal / Q000502:physiology; D016681:Genome, Fungal; D005838:Genotype; D020082:Magnaporthe / Q000235:genetics*; D008954:Models, Biological; D030781:Organisms, Genetically Modified; D010641:Phenotype; D037521:Virulence Factors / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology*
chemical_list: D037521:Virulence Factors