title: Efficient downregulation of alb1 gene using an AMA1-based episomal expression of RNAi construct in Aspergillus fumigatus.
abstract: An episomal RNAi silencing construct containing the inducible cbhB promoter and a hairpin structure has been made to downregulate the alb1 gene in the human pathogen Aspergillus fumigatus. Transformation of fungal protoplasts resulted in a high number of transformants with an inducible silenced phenotype (white spores). Efficient downregulation of the alb1 gene using this system suggests that this approach may overcome the variable downregulation observed with integrative constructs.
authors.affiliation: Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran. khalajs@pasteur.ac.ir
authors.forename: Vahid
authors.initials: V
authors.lastname: Khalaj
authors.forename: Hamid
authors.initials: H
authors.lastname: Eslami
authors.forename: Mohammad
authors.initials: M
authors.lastname: Azizi
authors.forename: Nuria
authors.initials: N
authors.lastname: Rovira-Graells
authors.forename: Mike
authors.initials: M
authors.lastname: Bromley
mesh_terms: D001232:Aspergillus fumigatus / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D002266:Carboxymethylcellulose Sodium / Q000494:pharmacology; D015536:Down-Regulation / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D005800:Genes, Fungal / Q000235:genetics*; D005947:Glucose / Q000494:pharmacology; D008957:Models, Genetic; D009154:Mutation; D010641:Phenotype; D010957:Plasmids / Q000235:genetics*; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D005947:Glucose; D002266:Carboxymethylcellulose Sodium