HpdR is a transcriptional activator of Sinorhizobium meliloti hpdA, which encodes a herbicide-targeted 4-hydroxyphenylpyruvate dioxygenase.
Sinorhizobium meliloti hpdA, which encodes the herbicide target 4-hydroxyphenylpyruvate dioxygenase, is positively regulated by HpdR. Gel mobility shift and DNase I footprinting analyses revealed that HpdR binds to a region that spans two conserved direct-repeat sequences within the hpdR-hpdA intergenic space. HpdR-dependent hpdA transcription occurs in the presence of 4-hydroxyphenylpyruvate, tyrosine, and phenylalanine, as well as during starvation.
Loprasert
Suvit
S
Laboratory of Biotechnology, Chulabhorn Research Institute, Vibhavadee-Rangsit Highway, Bangkok 10210, Thailand. suvit@cri.or.th
Whangsuk
Wirongrong
W
Dubbs
James M
JM
Sallabhan
Ratiboot
R
Somsongkul
Kumpanart
K
Mongkolsuk
Skorn
S
D015111:4-Hydroxyphenylpyruvate Dioxygenase / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D018983:DNA Footprinting; D004269:DNA, Bacterial / Q000378:metabolism; D021901:DNA, Intergenic / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D015964:Gene Expression Regulation, Bacterial; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012091:Repetitive Sequences, Nucleic Acid; D016962:Sinorhizobium meliloti / Q000235:genetics / Q000502:physiology*; D015534:Trans-Activators / Q000378:metabolism / Q000502:physiology*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D021901:DNA, Intergenic; D015534:Trans-Activators; D015111:4-Hydroxyphenylpyruvate Dioxygenase