title: Systematic investigation of the thermodynamics of HSA adsorption to N-iso-propylacrylamide/N-tert-butylacrylamide copolymer nanoparticles. Effects of particle size and hydrophobicity.
abstract: Nanoparticles in biological fluids almost invariably become coated with proteins that may confer nanomedical and nanotoxicological effects. Understanding these effects requires quantitative measurements using simple systems. Adsorption of HSA to copolymer nanoparticles of varying hydrophobicity and curvature was studied using ITC, yielding stoichiometry, affinity, and enthalpy changes upon binding. The hydrophobicity was controlled via the co-monomer ratio, N-iso-propylacrylamide/N-tert-butylacrylamide. The most hydrophobic particles become fully covered with a single layer of protein, except at high curvature.
authors.affiliation: Department of Biophysical Chemistry, Chemical Centre, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden.
authors.forename: Stina
authors.initials: S
authors.lastname: Lindman
authors.forename: Iseult
authors.initials: I
authors.lastname: Lynch
authors.forename: Eva
authors.initials: E
authors.lastname: Thulin
authors.forename: Hanna
authors.initials: H
authors.lastname: Nilsson
authors.forename: Kenneth A
authors.initials: KA
authors.lastname: Dawson
authors.forename: Sara
authors.initials: S
authors.lastname: Linse
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D000327:Adsorption; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003198:Computer Simulation; D003460:Crystallization / Q000379:methods; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008422:Materials Testing; D008956:Models, Chemical; D008968:Molecular Conformation; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry* / Q000648:ultrastructure; D013499:Surface Properties; D013816:Thermodynamics
chemical_list: D000178:Acrylamides; D020099:Coated Materials, Biocompatible; D012709:Serum Albumin; C067295:N-isopropylacrylamide; C115287:tert-butylacrylamide