Phylogenetic relationships of Auriculoscypha based on ultrastructural and molecular studies.
The phylogeny of Auriculoscypha anacardiicola, an associate of scale insects in India, is investigated using subcellular characters and MP and Bayesian analyses of combined nuLSU-rDNA, nuSSU-rDNA and 5.8S rDNA sequence data. It has simple septa with a pulley-wheel-shaped pore plug, which is diagnostic of phytoparasitic members of the Pucciniomycetes, and hyphal wall break on branching, a phenomenon unique to some simple septate heterobasidiomycetes. The septal ultrastructure of A. anacardiicola is similar to that of the genus Septobasidium. The close relationship to Septobasidium is also confirmed by rDNA sequence analyses. The polyphyletic nature of the order Platygloeales, noted in earlier studies, is evident from the present molecular analysis as well. The placement of Auriculoscypha in the Platygloeales can no longer be justified and both ultrastructural and molecular evidence strongly support the placement of Auriculoscypha in the Septobasidiales.
Kumar
T K Arun
TK
Department of Plant Biology, University of Minnesota, 1445 Gortner Avenue, St Paul, MN 55108-1095, USA. arunkumartk3@rediffmail.com
Celio
Gail J
GJ
Matheny
P Brandon
PB
McLaughlin
David J
DJ
Hibbett
David S
DS
Manimohan
P
P
D001487:Basidiomycota / Q000145:classification* / Q000235:genetics / Q000648:ultrastructure; D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D007194:India; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010802:Phylogeny; D012331:RNA, Fungal / Q000235:genetics; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012331:RNA, Fungal; D012340:RNA, Ribosomal, 5.8S