Lactobacillus rhamnosus GG exacerbates intestinal ulceration in a model of indomethacin-induced enteropathy.
Lactobacillus rhamnosus GG (LGG) and Bifidobacterium lactis Bb12 (Bb12) were assessed for their potential to prevent indomethacin-induced ulceration in the small intestine of Sprague-Dawley rats. Rats were gavaged skim milk, LGG, or Bb12 twice daily for 14 days. Between days 7-14, rats were gavaged indomethacin (Indo; 6 mg/kg). At sacrifice, small intestine was scored for ulceration and sampled for histologic, immunohistochemical, and myeloperoxidase (MPO) analyses. Indo+LGG-treated rats exhibited a 2.3-fold increase in MPO activity and a 9.8-fold increase in ulceration area compared to Indo-treated controls; these parameters did not differ significantly between Indo+Bb12 and Indo-treated controls. Crypt cell apoptosis decreased by 82% in Indo+Bb12-treated and 55% in Indo+LGG-treated rats compared to Indo-treated controls. Proliferation increased by 209% in Indo+LGG-treated animals compared to Indo-treated controls. Bb12 did not reduce indomethacin-induced intestinal ulceration, whereas LGG actually increased some indicators of injury. LGG and Bb12, at the doses tested, cannot alleviate indomethacin-induced intestinal injury.
Kamil
Rasha
R
entre for Paediatric and Adolescent Gastroenterology, Children, Youth and Women's Health Service, 72 King William Road, North Adelaide, South Australia, Australia.
Geier
Mark S
MS
Butler
Ross N
RN
Howarth
Gordon S
GS
D000818:Animals; D017209:Apoptosis; D001644:Bifidobacterium; D049109:Cell Proliferation; D004195:Disease Models, Animal; D007213:Indomethacin; D007410:Intestinal Diseases / Q000139:chemically induced / Q000201:enzymology / Q000382:microbiology* / Q000473:pathology / Q000517:prevention & control*; D007421:Intestine, Small / Q000201:enzymology / Q000382:microbiology* / Q000473:pathology; D052201:Lacticaseibacillus rhamnosus; D008297:Male; D010437:Peptic Ulcer / Q000139:chemically induced / Q000201:enzymology / Q000382:microbiology* / Q000473:pathology / Q000517:prevention & control*; D009195:Peroxidase / Q000032:analysis; D019936:Probiotics / Q000009:adverse effects*; D051381:Rats; D017207:Rats, Sprague-Dawley
D009195:Peroxidase; D007213:Indomethacin