title: Nogo-A is a reliable oligodendroglial marker in adult human and mouse CNS and in demyelinated lesions.
abstract: The unambiguous identification of oligodendrocytes in tissue sections, especially in myelinated tracts, is often difficult. Most of the antibodies used to identify oligodendrocytes label the myelin sheath as well. Originally described as an inhibitor of axonal outgrowth, Nogo-A is known to be strongly expressed in mature oligodendrocytes in vivo. In the present investigation we analyzed the expression patterns of Nogo-A in adult mouse and human CNS as well as in demyelinating animal models and multiple sclerosis lesions. Nogo-A expression was compared with that of other frequently used oligodendroglial markers such as CC1, CNP, and in situ hybridization for proteolipid protein mRNA. Nogo-A strongly and reliably labeled oligodendrocytes in the adult CNS as well as in demyelinating lesions and thus represents a valuable tool for the identification of oligodendrocytes in human and mouse CNS tissue.
authors.affiliation: Department of Neuropathology, University of Göttingen, Göttingen, Germany. tanjakuhlmann@gmx.de
authors.forename: Tanja
authors.initials: T
authors.lastname: Kuhlmann
authors.forename: Leah
authors.initials: L
authors.lastname: Remington
authors.forename: Brigitte
authors.initials: B
authors.lastname: Maruschak
authors.forename: Trevor
authors.initials: T
authors.lastname: Owens
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Brück
mesh_terms: D000328:Adult; D000818:Animals; D000941:Antigens / Q000378:metabolism; D015415:Biomarkers; D002490:Central Nervous System / Q000378:metabolism* / Q000473:pathology*; D004195:Disease Models, Animal; D004827:Epilepsy / Q000473:pathology; D005260:Female; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D009185:Myelin Proteins / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009444:Neuritis, Autoimmune, Experimental / Q000473:pathology*; D000070798:Nogo Proteins; D009836:Oligodendroglia / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism; D000099216:Chondroitin Sulfate Proteoglycan 4
chemical_list: D000941:Antigens; D015415:Biomarkers; D047908:Intracellular Signaling Peptides and Proteins; D009185:Myelin Proteins; D009419:Nerve Tissue Proteins; D000070798:Nogo Proteins; D011509:Proteoglycans; D000099216:Chondroitin Sulfate Proteoglycan 4; C000603199:RTN4 protein, human; C000603201:Rtn4 protein, mouse