The liver plays a key role in whole body sterol accretion of the neonatal Golden Syrian hamster.
Neonates have a significant requirement for cholesterol. From -1 to 25 days of age, the liver accrues 6.9 mg cholesterol and the extra-hepatic tissues accrue 107.7 mg cholesterol in the hamster. It is currently unknown if each of these body compartments synthesizes their own cholesterol or if they have alternative source(s) of sterol. Using (3)H(2)O, in vivo hepatic sterol synthesis rates (per g liver per animal) increased between -1 and 5 days of age, decreased by 10 days of age, and increased again by 15 days of age. HMG-CoA reductase (HMGR) expression levels paralleled in vivo synthesis rates. Extra-hepatic sterol synthesis rates followed the same pattern as sterol synthesis rates in the liver. When sterol synthesis rates were converted to the mass of sterol synthesized per day, the liver synthesized 38.9 and the extra-hepatic tissues synthesized 63.9 mg cholesterol in the 26-day neonatal period. Comparing the amount of cholesterol accrued to that synthesized, one can conclude that the liver is a major source of sterol for the whole body during the neonatal period of the hamster. These results may help elucidate the cause(s) of reduced growth rates in neonates with liver disease or in neonates with compromised sterol synthesis rates.
Yao
Lihang
L
Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, OH 45237-0507, USA.
Horn
Paul S
PS
Heubi
James E
JE
Woollett
Laura A
LA
D000818:Animals; D000831:Animals, Newborn; D001835:Body Weight; D008078:Cholesterol, LDL / Q000097:blood; D015243:Cholesterol, VLDL / Q000097:blood; D006224:Cricetinae; D005227:Fatty Acids / Q000096:biosynthesis; D005260:Female; D008099:Liver / Q000033:anatomy & histology / Q000378:metabolism*; D008297:Male; D008647:Mesocricetus / Q000378:metabolism*; D009929:Organ Size; D013261:Sterols / Q000096:biosynthesis / Q000378:metabolism*
D008078:Cholesterol, LDL; D015243:Cholesterol, VLDL; D005227:Fatty Acids; D013261:Sterols