[Molecular analysis on the S gene of three Crimean-Congo hemorrhagic fever virus strains in China].
OBJECTIVE
To compare the molecular characteristics of 3 Crimean-Congo hemorrhagic fever viruses(CCHFV) isolated in Xinjiang province.


METHODS
YL05035, YT05099 and LT05146 were isolated in 2005 from Hyalomma ticks and viral RNA was extracted from suckling mouse brains infected with these three strains respectively. The polymerase chain reaction(PCR) products of S segments from the 3 strains of CCHFV were directly sequenced.


RESULTS
The full-length'S RNA from the 3 strains of CCHFV all comprised 1673 nucleotides with ORF of them including 1449 nucleotides and encoding a protein which comprised 482 amino acids in a viral complementary sense. The sequences indicated that the three strains of CCHFV isolated from ticks in Xinjiang province were highly homologenic. Data from the phylogenetic analysis showed that the obtained sequences were identical. The homology between 3 strains of CCHFV was 99.5%. Their homologies compared with that of the other strains isolated from other region of Xinjiang were also high at nucleotide levels (92.7%-99.8%). The three strains which were clustered together with 7001 strain and 79121 strain (isolated from patient and rat in Xinjiang respectively) was only different by 2%-3%. The genetic difference from the prototype CCHFV Nigerian strain IBAR10200 was 13%. In comparison, the Nigerian CCHFV tick isolate was more divergent when compared with the reference China strains 66019 and with the three variants mentioned above.


CONCLUSIONS
The CCHFV isolated from China comprised a group of genetically high conserved strains.
Sun
Su-rong
SR
Key Laboratory of Molecular Biology, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China.
Meng
Wei-wei
WW
Zhang
Yu-jiang
YJ
Wang
Xin-hui
XH
Azati
Dai
Xiang
X
Zhu
Han-ping
HP
Feng
Chong-hui
CH
Ma
Ji
J
Zhang
Fu-chun
FC
Xie
Yan-xiang
YX
Hang
Chang-shou
CS
D000818:Animals; D001921:Brain / Q000821:virology; D002681:China; D005814:Genes, Viral; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000235:genetics* / Q000302:isolation & purification; D051379:Mice; D012367:RNA, Viral / Q000032:analysis
D012367:RNA, Viral