[Genetic control of intercellular adhesion or how cadherins shape the fruitfly Drosophila melanogaster].
The beauty and diversity of cell shapes have always fascinated both biologists and physicists. In the early 1950, J. Holtfreter coined the term "tissue affinities" to describe the forces behind the spontaneous shaping of groups of cells. These tissue affinites were later on related to adhesive properties of cell membranes. In the 1960, Malcom Steinberg proposed the differential adhesion hypothesis (DAH) as a physical explanation of the liquid-like behaviour of tissues and cells during morphogenesis. However, the link between the cellular properties of adhesion molecules, such as the cadherins, and the physical rules that shape the body, has remained unclear. Recent in vitro studies have now shown that surface tensions, which drive the spontaneous liquid-like behaviour of cell rearrangements, are a direct and linear function of cadherin expression levels. Tissue surface tensions thus arise from differences in intercellular adhesiveness, which validates the DAH in vitro. The DAH was also vindicated in vivo by stunning experiments in Drosophila. The powerful genetic tools available in Drosophila allow to manipulate the levels and patterns of expression of several cadherins and to create artificially differences in intercellular adhesiveness. The results showed that simple laws of thermodynamics, as well as quantitative and qualitative differences in cadherins expression were sufficient to explain processes as complex as the establishment of the anterior-posterior axis and the formation of the compound eye in Drosophila.
Bécam
Isabelle
I
Institut Jacques-Monod, CNRS, Universités Paris 6 et 7, 2, place Jussieu, 75251 Paris Cedex 05, France.
Huynh
Jean-René
JR
D000818:Animals; D019521:Body Patterning / Q000502:physiology*; D015820:Cadherins / Q000502:physiology*; D002448:Cell Adhesion / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000502:physiology*; D019070:Cell Lineage; D002642:Chick Embryo; D029721:Drosophila Proteins / Q000502:physiology*; D004331:Drosophila melanogaster / Q000166:cytology / Q000196:embryology / Q000254:growth & development*; D005123:Eye / Q000166:cytology / Q000254:growth & development; D005136:Eye Proteins / Q000502:physiology; D005260:Female; D009865:Oocytes / Q000166:cytology; D006080:Ovarian Follicle / Q000166:cytology / Q000378:metabolism; D013500:Surface Tension; D013816:Thermodynamics
D015820:Cadherins; D015815:Cell Adhesion Molecules; D029721:Drosophila Proteins; D005136:Eye Proteins; C501889:shg protein, Drosophila