Development of an automated SNP analysis method using a paramagnetic beads handling robot.
Biological and medical importance of the single nucleotide polymorphism (SNP) has led to development of a wide variety of methods for SNP typing. Aiming for establishing highly reliable and fully automated SNP typing, we have developed the adapter ligation method in combination with the paramagnetic beads handling technology, Magtration(R). The method utilizes sequence specific ligation between the fluorescently labeled adapter and the sample DNAs at the cohesive end produced by a type IIS restriction enzyme. Evaluation of the method using human genomic DNA showed clear discrimination of the three genotypes without ambiguity using the same reaction condition for any SNPs examined. The operations following PCR amplification were automatically performed by the Magtration(R)-based robot that we have previously developed. Multiplex typing of two SNPs in a single reaction by using four fluorescent dyes was successfully preformed at the almost same sensitivity and reliability as the single typing. These results demonstrate that the automated paramagnetic beads handling technology, Magtration(R), is highly adaptable to the automated SNP analysis and that our method best fits to an automated in-house SNP typing for laboratory and medical uses.
Hagiwara
Hiroko
H
Advanced Institute of Industrial Science and Technology (AIST), Japan.
Sawakami-Kobayashi
Kazumi
K
Yamamoto
Midori
M
Iwasaki
Shoji
S
Sugiura
Mika
M
Abe
Hatsumi
H
Kunihiro-Ohashi
Sumiko
S
Takase
Kumiko
K
Yamane
Noriko
N
Kato
Kaoru
K
Son
Renkon
R
Nakamura
Michihiro
M
Segawa
Osamu
O
Yoshida
Mamiko
M
Yohda
Masafumi
M
Tajima
Hideji
H
Kobori
Masato
M
Takahama
Yousuke
Y
Itakura
Mitsuo
M
Machida
Masayuki
M
D004867:Equipment Design / Q000295:instrumentation; D005820:Genetic Testing; D015894:Genome, Human; D005838:Genotype; D006801:Humans; D008280:Magnetics; D016133:Polymerase Chain Reaction / Q000295:instrumentation*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012371:Robotics / Q000295:instrumentation*; D017422:Sequence Analysis, DNA / Q000295:instrumentation*