Chromate reduction by Burkholderia cepacia MCMB-821, isolated from the pristine habitat of alkaline crater lake.
The Cr(VI)-reducing bacterial strain MCMB-821 was isolated from the alkaline crater lake of Lonar and was identified as Burkholderia cepacia. MCMB-821 was resistant to 1,000-ppm Cr(VI) and reduced 98% of the 75 ppm Cr(VI) within 36 h at pH 9.0 in the presence of 2% salt and lactose as the electron donor. The chromate-reducing efficiency of MCMB-821 was comparable under both aerobic as well as anaerobic conditions. Electron paramagnetic resonance spectroscopy data suggested that MCMB-821 reduced Cr(VI) to Cr(III) via the formation of transient Cr(V) intermediate. The chromate-reducing ability of MCMB-821 was suppressed in the presence of membrane inhibitors and enhanced in the presence of 2,4-dinitrophenol, suggesting the involvement of electron transport chain in the Cr(VI) bioreduction.
Wani
Revati
R
Biochemistry Division, Department of Chemistry, University of Pune, Ganeshkhind, Pune, 411007, India.
Kodam
K M
KM
Gawai
K R
KR
Dhakephalkar
P K
PK
D019297:2,4-Dinitrophenol / Q000494:pharmacology; D000968:Antimycin A / Q000494:pharmacology; D001386:Azides / Q000494:pharmacology; D016956:Burkholderia cepacia / Q000302:isolation & purification / Q000378:metabolism*; D002840:Chromates / Q000737:chemistry / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D004579:Electron Transport / Q000187:drug effects; D005618:Fresh Water / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction
D001386:Azides; D002840:Chromates; D000968:Antimycin A; D019297:2,4-Dinitrophenol