Frequent epigenetic silencing of protocadherin 10 by methylation in multiple haematologic malignancies.
Epigenetic silencing of tumour suppressor genes (TSG) inactivates TSG functions. Previously, we identified PCDH10 as a methylated TSG in carcinomas. Here, we detected its frequent silencing and methylation in lymphoma cell lines including 100% Burkitt, 100% diffuse large B cell, 86% Hodgkin, 100% nasal natural killer/T-cell lymphoma and 1/3 of leukaemia cell lines, and in primary tumours but not in normal peripheral blood mononuclear cells or lymph nodes. PCDH10 silencing could be reversed by demethylation with 5-aza-2'-deoxycytidine. Methylation was further detected in 14% of Hodgkin lymphoma sera. Thus, PCDH10 methylation is frequently involved in lymphomagenesis and could serve as a tumour-specific biomarker.
Ying
Jianming
J
Cancer Epigenetics Laboratory, Department of Clinical Oncology, The Chinese University of Hong Kong, Hong Kong.
Gao
Zifen
Z
Li
Hongyu
H
Srivastava
Gopesh
G
Murray
Paul G
PG
Goh
Hwee Koon
HK
Lim
Chai Yen
CY
Wang
Yajun
Y
Marafioti
Teresa
T
Mason
David Y
DY
Ambinder
Richard F
RF
Chan
Anthony T C
AT
Tao
Qian
Q
D014408:Biomarkers, Tumor; D002051:Burkitt Lymphoma / Q000235:genetics; D015820:Cadherins / Q000235:genetics*; D045744:Cell Line, Tumor; D018899:CpG Islands; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D019337:Hematologic Neoplasms / Q000235:genetics*; D006689:Hodgkin Disease / Q000235:genetics; D006801:Humans; D007938:Leukemia / Q000235:genetics; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics; D016399:Lymphoma, T-Cell / Q000235:genetics; D000091363:Protocadherins; D020133:Reverse Transcriptase Polymerase Chain Reaction
D014408:Biomarkers, Tumor; D015820:Cadherins; C438603:PCDH10 protein, human; D000091363:Protocadherins