Association of a functional polymorphism in the mu-opioid receptor gene with alcohol response and consumption in male rhesus macaques.
BACKGROUND
Innate differences in opioid neurotransmission are hypothesized to influence abuse liability of alcohol. In humans, a variant of the mu-opioid receptor gene (OPRM1A118G) increases receptor affinity, alcohol-induced euphoria, and risk for alcohol use disorders.


OBJECTIVE
To determine whether a variant in the mu-opioid receptor gene (OPRM1C77G) that increases affinity of the receptor is associated with alcohol response and consumption in macaques.


METHODS
Young adult rhesus macaques (Macaca mulatta) were intravenously administered 2.0 to 2.1 g of ethanol per kilogram of body weight and assessed for alcohol response. Animals were later given simultaneous access to an aspartame-sweetened 8.4% (vol/vol) ethanol solution and a vehicle for 1 hour per day, 5 days a week, for a period of 6 weeks. Animals (N = 82) were genotyped for the OPRM1C77G polymorphism; the effects of the genotype on alcohol response and consumption were determined by analysis of variance, with sex included as a nominal independent variable.


METHODS
Alcohol response (ataxia, stimulation, and sedation), average alcohol consumption, the percentage of days during which an animal consumed alcohol at a level sufficient to produce intoxication (> or =0.67 g of alcohol per kilogram of body weight), and alcohol preference (calculated as 100 x {alcoholic solution/[alcoholic solution + nonalcoholic solution]}).


RESULTS
Increased alcohol-induced stimulation was observed among male macaques carrying the OPRM1C77G allele. OPRM1C77G allele carriers consumed more ethanol and exhibited increased ethanol preference. Male carriers of the OPRM1C77G allele exhibited higher alcohol preference and consumption, and drank to intoxication more frequently than did C/C males.


CONCLUSIONS
These findings demonstrate that the rhesus macaques' equivalent of the OPRM1A118G variant is associated with increased alcohol response, consumption, and preference. Our results reveal effects of the OPRM1C77G genotype to be male-restricted or more marked among male macaques. This is of interest, given the fact that early-onset type II alcoholism is more common among men and that, among addicted individuals, men are more responsive to mu-opioid receptor blockade.
Barr
Christina S
CS
Laboratory of Clinical and Translational Studies, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Poolesville, MD 20837, USA. cbarr@mail.nih.gov
Schwandt
Melanie
M
Lindell
Stephen G
SG
Chen
Scott A
SA
Goldman
David
D
Suomi
Stephen J
SJ
Higley
J Dee
JD
Heilig
Markus
M
D017668:Age of Onset; D000428:Alcohol Drinking / Q000235:genetics*; D000437:Alcoholism / Q000453:epidemiology; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects* / Q000502:physiology; D003216:Conditioning, Operant / Q000187:drug effects / Q000502:physiology; D004195:Disease Models, Animal; D004327:Drinking Behavior / Q000187:drug effects / Q000502:physiology; D000431:Ethanol / Q000494:pharmacology*; D014644:Genetic Variation / Q000235:genetics; D005838:Genotype; D008253:Macaca mulatta / Q000235:genetics*; D008297:Male; D011110:Polymorphism, Genetic / Q000235:genetics*; D017450:Receptors, Opioid, mu / Q000235:genetics*; D012737:Sex Factors
D017450:Receptors, Opioid, mu; D000431:Ethanol