[Study on the correlationship between the expression of nm23H1mRNA, TGF-beta1mRNA and tumor metastases, survival rate with prostate cancer].
All the specimens were marked by CD31 antibody, the expression of nm23HlmRNA, TGF-beta1mRNA was detected in 42 cases of prostate cancer in situ hybridization with EnVision and Leica-Qwin computer image analysis system. The Weidner's highest vessel density counting method was used to analysis the microvessel density(MVD) of the specimens,and the patients' viability rate after operation was investigated. The correlation between the expression of nm23H1mRNA, TGF-betamRNA,CD31 and neoangiogenesis, hyperplasia of microvessel, tumor metastases in prostate cancer was studied. The positive expression of nm23H1mRNA in prostate cancer was 66.67% (28 cases). There was negative correlation between the positive expression of nm23H1mRNA and bone metastasizing, TNM staging,MVD in prostate cancer (P < 0.05). The positive expression of TGF-beta1mRNA was 78.75% (33 cases), and was positive correlative to the bone metastasis, TNM stages, MVD in prostate cancer (P < 0.05). It is significantly different (P < 0.01 or P < 0.05) with that in adjacent nontumorous tissue. The MVD (78.51 +/- 10.29/mm2) in prostate cancer was significantly (P < 0.05) higher than that in adjacent nontumorous tissue (34.19 +/- 9.27/mm2). The MVD (92.41 +/- 15.42/mm2) in those who died in five years (18 cases) was significantly higher (P < 0.05) than that (62.79 +/- 13.58/mm2) in those who were still alive. In tumor pathology grading,different differentiation, the difference of nm23H1mRNA positive expression was statistically significant (P < 0.05). Higher the nm23H1mRNA expressed,lower the bone metastasized,and higher the viability rate was. Therefore, the nm23H1 gene was thought to have the effect of suppressing prostate cancer occurring and bone metastasizing. Higher the TGF-beta1mRNA expressed, higher the tumor bone metastasized, and lower the viability rate was. Therefore, the TGF-beta1 gene was thought to have the promote genesis and metastasize of prostate cancer. There is close correlation between the CD31 expression, MVD and prostate cancer bone metastasizing. The higher MVD level indicated neoangiogenesis in prostate cancer. TGF-beta1mRNA promote neoangiogenesis induced by tumor and play an important role in bone metastasizing.
Ding
Guo Fang
GF
Medical College of Zhejiang Ocean University, Zhoushan.
Li
Ji Cheng
JC
Xu
Yin Feng
YF
D000368:Aged; D000369:Aged, 80 and over; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D054778:NM23 Nucleoside Diphosphate Kinases / Q000235:genetics*; D009362:Neoplasm Metastasis; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015996:Survival Rate; D053773:Transforming Growth Factor beta1 / Q000235:genetics*
D054778:NM23 Nucleoside Diphosphate Kinases; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; C517938:NME1 protein, human