title: Alteration of myocardial sarcoplasmic reticulum Ca2+-ATPase and Na+-Ca2+ exchanger expression in human left ventricular volume overload.
abstract: BACKGROUND
  Reduced myocardial contractility is often attributed to altered Ca(2+) transients and expression of Ca(2+)-ATPase of the SR (SERCA) and Na+/Ca(2+)exchanger (NCX) genes.
  
  
  OBJECTIVE
  To assess myocardial expression of SERCA and NCX protein levels in left ventricular (LV) remodelling due to chronic severe mitral regurgitation (MR).
  
  
  METHODS
  Myocardial expression of SERCA/NCX in biopsy specimens obtained during mitral surgery was assessed in 36 MR patients with LV remodelling and plasma neurohumoral/cytokine activation and in four non-failing hearts (NFH).
  
  
  RESULTS
  Myocardial protein levels of SERCA were significantly (20%) lower in the MR group than in NFH group (p=0.016). No significant changes in NCX were observed. However, a lack of homogeneity with regard to SERCA/NCX proteins was observed. Moreover, SERCA was negatively correlated with BNP (r=-0.49, p=0.02), TNFalpha (r=-0.68, p=0.0005) and IL-6 (r=-0.52, p=0.02), whereas NCX was only negatively correlated with TNFalpha (r=-0.62, p=0.002).
  
  
  CONCLUSIONS
  MR patients showed wide variations in SERCA/NCX protein expression. Myocardial protein levels of SERCA were significantly lower in the MR population. Moreover, a correlation between BNP, cytokines (IL-6, TNFalpha) and the expression of SERCA/NCX proteins was observed.
authors.affiliation: Heart Failure and Transplantology Department, National Institute of Cardiology, 04-628 Warsaw, Alpejska 42 St, Poland. leszekp@ikard.pl <leszekp@ikard.pl>
authors.forename: Przemyslaw
authors.initials: P
authors.lastname: Leszek
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Szperl
authors.forename: Anna
authors.initials: A
authors.lastname: Klisiewicz
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Janas
authors.forename: Andrzej
authors.initials: A
authors.lastname: Biederman
authors.forename: Tomasz
authors.initials: T
authors.lastname: Rywik
authors.forename: Walerian
authors.initials: W
authors.lastname: Piotrowski
authors.forename: Mieczyslaw
authors.initials: M
authors.lastname: Kopacz
authors.forename: Jerzy
authors.initials: J
authors.lastname: Korewicki
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D016207:Cytokines / Q000097:blood; D005260:Female; D015870:Gene Expression / Q000502:physiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D008944:Mitral Valve Insufficiency / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology; D009200:Myocardial Contraction / Q000235:genetics*; D018377:Neurotransmitter Agents / Q000097:blood; D010210:Papillary Muscles / Q000473:pathology / Q000503:physiopathology; D012519:Sarcoplasmic Reticulum / Q000235:genetics* / Q000473:pathology; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000235:genetics*; D019831:Sodium-Calcium Exchanger / Q000235:genetics*; D018487:Ventricular Dysfunction, Left / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology; D020257:Ventricular Remodeling / Q000235:genetics* / Q000502:physiology
chemical_list: D016207:Cytokines; D018377:Neurotransmitter Agents; D019831:Sodium-Calcium Exchanger; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases