Identification by surface plasmon resonance of the mycobacterial lipomannan and lipoarabinomannan domains involved in binding to CD14 and LPS-binding protein.
The mycobacterial lipoglycans, lipomannan (LM) and lipoarabinomannan (LAM), regulate host defence mechanisms through their interaction with pattern recognition receptors such as Toll-like receptors (TLRs). We have developed a surface plasmon resonance assay to analyse the molecular basis for the recognition of Mycobacterium kansasii LM or LAM, by immobilized CD14 and LPS-binding protein (LBP) both being capable to promote presentation of bacterial glycolipids to TLRs. The affinity of either LM/LAM was higher to CD14 than to LBP. Kinetic and Scatchard analyses were consistent with a model involving a single class of binding sites. These interactions required the lipidic anchor, but not the carbohydrate domains, of LM or LAM. We also provide evidence that addition of recombinant LBP enhanced the stimulatory effect of LM or LAM on matrix metalloproteinase-9 expression and secretion in macrophages, through a TLR1/TLR2-dependent mechanism.
Elass
Elisabeth
E
Unité Mixte de Recherche n 8576 du Centre National de la Recherche Scientifique, Institut Fédératif de Recherche 147, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq Cedex, France. elisabeth.elass@univ-lille1.fr
Coddeville
Bernadette
B
Guérardel
Yann
Y
Kremer
Laurent
L
Maes
Emmanuel
E
Mazurier
Joël
J
Legrand
Dominique
D
D000209:Acute-Phase Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D019909:Mycobacterium kansasii; D017434:Protein Structure, Tertiary; D020349:Surface Plasmon Resonance; D051194:Toll-Like Receptor 1 / Q000276:immunology; D051195:Toll-Like Receptor 2 / Q000276:immunology; D000099231:Lipopolysaccharide-Binding Protein
D000209:Acute-Phase Proteins; D002352:Carrier Proteins; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D020780:Matrix Metalloproteinase 9; D008562:Membrane Glycoproteins; D051194:Toll-Like Receptor 1; D051195:Toll-Like Receptor 2; D000099231:Lipopolysaccharide-Binding Protein; C050016:lipoarabinomannan; C064351:lipomannan