EGO, a novel, noncoding RNA gene, regulates eosinophil granule protein transcript expression.
Gene expression profiling of early eosinophil development shows increased transcript levels of proinflammatory cytokines, chemokines, transcription factors, and a novel gene, EGO (eosinophil granule ontogeny). EGO is nested within an intron of the inositol triphosphate receptor type 1 (ITPR1) gene and is conserved at the nucleotide level; however, the largest open reading frame (ORF) is 86 amino acids. Sucrose density gradients show that EGO is not associated with ribosomes and therefore is a noncoding RNA (ncRNA). EGO transcript levels rapidly increase following interleukin-5 (IL-5) stimulation of CD34(+) hematopoietic progenitors. EGO RNA also is highly expressed in human bone marrow and in mature eosinophils. RNA silencing of EGO results in decreased major basic protein (MBP) and eosinophil derived neurotoxin (EDN) mRNA expression in developing CD34(+) hematopoietic progenitors in vitro and in a CD34(+) cell line model. Therefore, EGO is a novel ncRNA gene expressed during eosinophil development and is necessary for normal MBP and EDN transcript expression.
Wagner
Lori A
LA
School of Medicine, Department of Dermatology, University of Utah, Salt Lake City, Utah 84132, USA. lori.wagner@hsc.utah.edu
Christensen
Clarissa J
CJ
Dunn
Diane M
DM
Spangrude
Gerald J
GJ
Georgelas
Ann
A
Kelley
Linda
L
Esplin
M Sean
MS
Weiss
Robert B
RB
Gleich
Gerald J
GJ
D002478:Cells, Cultured; D047091:Eosinophil Granule Proteins / Q000235:genetics*; D047093:Eosinophil Major Basic Protein / Q000235:genetics*; D047208:Eosinophil-Derived Neurotoxin / Q000235:genetics*; D004804:Eosinophils / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000235:genetics*; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D006801:Humans; D053496:Inositol 1,4,5-Trisphosphate Receptors / Q000235:genetics; D022661:RNA, Untranslated / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic
D047091:Eosinophil Granule Proteins; D053496:Inositol 1,4,5-Trisphosphate Receptors; D022661:RNA, Untranslated; D047208:Eosinophil-Derived Neurotoxin; D047093:Eosinophil Major Basic Protein