[Exploration of the alternative splicing variants of rat phospholipase C-gamma 1 pre-mRNA].
OBJECTIVE
To explore the expression of phospholipase C-gamma 1 (PLC-gamma1) alternative splicing variants in rats.


METHODS
According to the sequence of human PLCG1 splicing variant, specific primers for rat PLC-gamma1 were designed and synthesized. The rat RNA was reverse transcribed into cDNA, which was amplified using the specific primers, and the PCR products were sequenced and analyzed using BLAST and bioinformatics methods. Totally 21 rat tissue samples were examined, including the heart, liver, lung, kidney, eyeball, and brain obtained in 3 different embryonic stages, 7 different early postnatal stages, and in adulthood.


RESULTS
The result did not show that rat PLC-gamma1 had the same splicing variant (PLC-gamma1a, NM_002660) as human does.


CONCLUSIONS
The same splicing variant of PLC-gamma1 detectable in human may not exist in rats, and the pre-mRNA may undergo splicing resulting predominantly in PLC-gamma1b mRNA. Very likely, the alternative splicing site of rat PLC-gamma1 is not identical to that of human.
Liu
Zhong-Ying
ZY
Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China. lzylele@yahoo.com.cn
Luo
Shen-Qiu
SQ
Zhao
Yong-Zhong
YZ
D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D008969:Molecular Sequence Data; D051966:Phospholipase C gamma / Q000235:genetics*; D012322:RNA Precursors / Q000235:genetics*; D022821:RNA Splice Sites / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D012322:RNA Precursors; D022821:RNA Splice Sites; D051966:Phospholipase C gamma