title: Proteomic analysis of salivary acidic proline-rich proteins in human preterm and at-term newborns.
abstract: A 1 year follow-up investigation of salivary acidic proline-rich proteins (aPRPs) in preterm and at-term newborns using HPLC-ESI-IT-MS showed that (i) this class of proteins is constitutive rather than inducible, as it is still found in the oral cavity of preterm newborns from 180 days of postconception age (PCA); (ii) the expression of PRH-2 locus anticipates that of PRH-1, since Db isoforms are expressed some months after the PRP-1 and PRP-2 isoforms. The evaluation of the relative abundances of the different aPRPs isoforms and derivatives (differently phosphorylated and cleaved) as a function of PCA showed that (iii) the proteolytic enzymes generating truncated isoforms are also constitutive because they are fully active since 180 days of PCA; (iv) the kinase involved in aPRP phosphorylation is not fully mature in preterm newborns, but its activity increases with PCA, synchronizing with that of at-term newborns and reaching the adult levels at about 500-600 days of PCA, in concomitance with the beginning of deciduous dentition.
authors.affiliation: Istituto di Biochimica e Biochimica Clinica, Istituto Scientifico Internazionale Paolo VI di ricerca sulla fertilità ed infertilità umana, Roma, Italy.
authors.forename: Rosanna
authors.initials: R
authors.lastname: Inzitari
authors.forename: Giovanni
authors.initials: G
authors.lastname: Vento
authors.forename: Ettore
authors.initials: E
authors.lastname: Capoluongo
authors.forename: Simona
authors.initials: S
authors.lastname: Boccacci
authors.forename: Chiara
authors.initials: C
authors.lastname: Fanali
authors.forename: Tiziana
authors.initials: T
authors.lastname: Cabras
authors.forename: Costantino
authors.initials: C
authors.lastname: Romagnoli
authors.forename: Bruno
authors.initials: B
authors.lastname: Giardina
authors.forename: Irene
authors.initials: I
authors.lastname: Messana
authors.forename: Massimo
authors.initials: M
authors.lastname: Castagnola
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007231:Infant, Newborn / Q000378:metabolism*; D007234:Infant, Premature / Q000378:metabolism*; D009055:Mouth / Q000737:chemistry / Q000378:metabolism; D010455:Peptides / Q000032:analysis* / Q000378:metabolism; D010766:Phosphorylation; D047928:Premature Birth; D055232:Proline-Rich Protein Domains; D020033:Protein Isoforms / Q000032:analysis / Q000378:metabolism; D040901:Proteomics; D012463:Saliva / Q000737:chemistry / Q000378:metabolism; D055273:Salivary Proline-Rich Proteins; D012471:Salivary Proteins and Peptides / Q000032:analysis* / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: C524536:PRH1 protein, human; D010455:Peptides; D020033:Protein Isoforms; D055273:Salivary Proline-Rich Proteins; D012471:Salivary Proteins and Peptides