title: [Preparation and characterization of the monoclonal antibody against human soluble mesothelin-related proteins].
abstract: OBJECTIVE
  To prepare the monoclonal antibody (mAb) against human soluble mesothelin-related proteins (SMR) and identify its properties.
  
  
  METHODS
  The B-cell epitopes of mesothelin (MSLN) were predicted by multi-parameter prediction method. Then BALB/c mice were immunized with compound MSLN polypeptide prepare the mAb by hybridoma technique. The specificity of antibody was evaluated by immuocytochemistry and Western blot respectively.
  
  
  RESULTS
  Using the multi-parameter prediction of B-cell epitopes, the 471-481 epitope domain was selected and synthesized. Then the mice were immunized and mAb against human SMR was prepared and designated 2H10. The specificity of mAb 2H10 was evaluated to be high by using immuocytochemistry and Western blot.
  
  
  CONCLUSIONS
  The successful preparation of the mAb against SMR will provide efficient reagent for further detection of SMR by ELISA.
authors.affiliation: Department of Obstetrics and Gynaecology, the Fourth Hosipital of Hebei Medical University, Shijiazhuang, China.
authors.forename: Shu-Cheng
authors.initials: SC
authors.lastname: Chen
authors.forename: Ya-Kun
authors.initials: YK
authors.lastname: Liu
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Wu
authors.forename: Bao-En
authors.initials: BE
authors.lastname: Shan
authors.forename: Xing
authors.initials: X
authors.lastname: Chen
authors.forename: Dong-Xia
authors.initials: DX
authors.lastname: Sun
authors.forename: Li
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D015153:Blotting, Western; D058851:GPI-Linked Proteins; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007150:Immunohistochemistry; D008562:Membrane Glycoproteins / Q000276:immunology* / Q000378:metabolism; D000090204:Mesothelin; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000911:Antibodies, Monoclonal; D058851:GPI-Linked Proteins; C000715527:MSLN protein, human; D008562:Membrane Glycoproteins; C000715528:Msln protein, mouse; D000090204:Mesothelin