Molecular cloning and functional analysis of goose interferon gamma.
The cDNA for goose interferon gamma (goIFN-gamma) was cloned from PHA-stimulated goose peripheral blood mononuclear cells (PBMCs) by RT-PCR. This cDNA encodes a 19-amino acid signal peptide and a 145-amino acid mature protein, which shares a high homology with duck IFN-gamma. Recombinant mature goose interferon gamma (rgoIFN-gamma) generated from both prokaryotic and eukaryotic expression systems effectively inhibited the replication of goose paramyxovirus and recombinant vesicular stomatitis virus in vitro. These antiviral activities were abrogated by rabbit anti-rgoIFN-gamma antibodies in vitro. Furthermore, rgoIFN-gamma stimulated goose peritoneal macrophages to produce nitric oxide (NO) in vitro, demonstrating its macrophage activating factor (MAF) activity. Therefore, the availability of bioactive rgoIFN-gamma and its specific antibodies provides valuable tools for studying T cell immunity in geese.
Li
Hong-Tao
HT
Department of Preventive Veterinary Medicine, College of Animal Medicine, Northeast Agricultural University, Harbin 150030, China.
Ma
Bo
B
Mi
Jing-Wei
JW
Jin
Hong-Yan
HY
Xu
Li-Na
LN
Wang
Jun-Wei
JW
D000595:Amino Acid Sequence; D000818:Animals; D018109:Avulavirus / Q000502:physiology; D016367:Baculoviridae / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005777:Geese / Q000235:genetics* / Q000276:immunology*; D007371:Interferon-gamma / Q000235:genetics* / Q000276:immunology* / Q000494:pharmacology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D014721:Vesicular stomatitis Indiana virus / Q000502:physiology; D014779:Virus Replication / Q000187:drug effects
D012333:RNA, Messenger; D011994:Recombinant Proteins; D007371:Interferon-gamma