title: [Multi-components quantitation by one marker new method for quality evaluation of Chinese herbal medicine].
abstract: OBJECTIVE
  To establish a new quality evaluation method for Chinese herbal medicine, using one chemical reference substance to calculate multi-components simultaneously.
  
  
  METHODS
  Relative correction factors (RCF) of mutongsaponin B and C to saponin PJ1, a characteristic component as marker, were calcalated in The chromatographic conditions for determination of the three saponins in Caulis Akebiae akebiae as a research model. The contents of saponin PJ1 were determined by external standard method, and those of mutongsaponin B and C were calculated by saponin PJ1 and their RCF. The accuracy of the new method was evaluated by comparing the calculated contents with the determined contents.
  
  
  RESULTS
  The analysis procedures were validated. There has been no significant difference between the calculated contents and the determined contents, according to the angle cosine value, and the calcalated RCF have confidence value.
  
  
  CONCLUSIONS
  The method of multi-components quantitation by one marker has been verified in caulis akebiae and it is to be a new quality evaluation pattern for Chinese herbal medicines.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medicial Sciences, Beijing 100700, China.
authors.forename: Zhi-min
authors.initials: ZM
authors.lastname: Wang
authors.forename: Hui-min
authors.initials: HM
authors.lastname: Gao
authors.forename: Xue-tao
authors.initials: XT
authors.lastname: Fu
authors.forename: Wei-hao
authors.initials: WH
authors.lastname: Wang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000592:standards; D006801:Humans; D019684:Magnoliopsida / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis
chemical_list: C526388:2,3,23-trihydroxyurs-12-en-28-oic acid O-xylopyranosyl-1-3-rhamnopyranosyl-1-4-glucopyranosyl-1-6-glucopyranosyl ester; D004365:Drugs, Chinese Herbal; D012503:Saponins