A role for DHX32 in regulating T-cell apoptosis.
DHX32 is a novel putative RNA helicase with an activation-dependent pattern of expression in T-cells. To gain insight into the role of DHX32, Jurkat-DHX32 cells, a stable Jurkat T-cell line with constitutive DHX32 expression, were generated by retroviral gene transfer. There were no significant differences between control and Jurkat-DHX32 cells in terms of proliferation and response to several chemotherapeutic agents. There was an altered response of Jurkat-DHX32 cells to Fas signaling associated with down-regulation of the anti-apoptotic protein c-FLIP short. In normal peripheral blood lymphocytes, a correlation between DHX32 and c-FLIP short expression was detected in response to different T-cell specific and non-specific activation stimuli. Our results suggest that DHX32 might be involved in regulating T-cell response to certain apoptotic stimuli.
Alli
Zaman
Z
Division of Haematopathology, Department of Paediatric Laboratory Medicine, Hospital for Sick Children, Toronto, ON, Canada.
Chen
Yong
Y
Abdul Wajid
Sarah
S
Al-Saud
Bandar
B
Abdelhaleem
Mohamed
M
D017209:Apoptosis / Q000502:physiology*; D053446:CASP8 and FADD-Like Apoptosis Regulating Protein / Q000096:biosynthesis; D053487:DEAD-box RNA Helicases / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D019169:Jurkat Cells; D008213:Lymphocyte Activation; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000166:cytology* / Q000201:enzymology* / Q000276:immunology; D019014:fas Receptor / Q000378:metabolism
D053446:CASP8 and FADD-Like Apoptosis Regulating Protein; C506386:CFLAR protein, human; D019014:fas Receptor; C466365:DHX32 protein, human; D053487:DEAD-box RNA Helicases