title: Yeast proteome map (update 2006).
abstract: To improve the potential of two-dimensional gel electrophoresis for proteomic investigations in yeast we have undertaken the systematic identification of Saccharomyces cerevisiae proteins separated on 2-D gels. We report here the identification of 187 novel protein spots. They were identified by two methods, mass spectrometry and gene inactivation. These identifications extend the number of protein spots identified on our yeast 2-D proteome map to 602, i.e. nearly half the detectable spots of the proteome map. These spots correspond to 417 different proteins. The reference map and the list of identified proteins can be accessed on the Yeast Protein Map server (www.ibgc.u-bordeaux2.fr/YPM).
authors.affiliation: Institut de Biochemie et Génétique Cellulaires, UMR CNRS 5095, Bordeaux, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Perrot
authors.forename: Anne-Laure
authors.initials: AL
authors.lastname: Guieysse-Peugeot
authors.forename: Aurélie
authors.initials: A
authors.lastname: Massoni
authors.forename: Christelle
authors.initials: C
authors.lastname: Espagne
authors.forename: Stéphane
authors.initials: S
authors.lastname: Claverol
authors.forename: Raquel Monteiro
authors.initials: RM
authors.lastname: Silva
authors.forename: Paul
authors.initials: P
authors.lastname: Jenö
authors.forename: Manuel
authors.initials: M
authors.lastname: Santos
authors.forename: Marc
authors.initials: M
authors.lastname: Bonneu
authors.forename: Hélian
authors.initials: H
authors.lastname: Boucherie
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D020868:Gene Silencing; D016681:Genome, Fungal; D013058:Mass Spectrometry; D010449:Peptide Mapping; D020543:Proteome; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*
chemical_list: D020543:Proteome; D029701:Saccharomyces cerevisiae Proteins