Evaluation of GSH adducts of adrenaline in biological samples.
The sustained high release of catecholamines to circulation is a deleterious condition that may induce toxicity, which seems to be partially related to the products formed by oxidation of catecholamines that can be further conjugated with glutathione (GSH). The aim of the present study was to develop a method for the determination of GSH adducts of adrenaline in biological samples. Two position isomers of the glutathion-S-yl-adrenaline were synthesized and characterized by HPLC using diode array, coulometric and mass detectors. A method for the extraction of these adducts from human plasma was also developed, based on adsorption to activated alumina, which showed adequate recoveries and proved to be crucial in removing interferences from plasma. The selectivity, precision and linearity of the method were all within the accepted values for these parameters. Furthermore, the sensitivity of this method allows the detection of adduct amounts that are within the range of the expected concentrations for these adducts under certain pathophysiological conditions and/or drug treatments. In conclusion, the development of this method allows the direct analysis of GSH adducts of adrenaline in human plasma, providing a valuable tool for the study of the catecholamine oxidation process and its related toxicity.
Silva
Renata
R
REQUIMTE, Serviço de Toxicologia, Faculdade de Farmácia, Universidade do Porto, Rua Aníbal Cunha, 164, 4099-030 Porto, Portugal.
Boldt
Sílvia
S
Costa
Vera Marisa
VM
Carmo
Helena
H
Carvalho
Márcia
M
Carvalho
Félix
F
Bastos
Maria de Lourdes
Mde L
Lemos-Amado
Francisco
F
Remião
Fernando
F
D002851:Chromatography, High Pressure Liquid; D004837:Epinephrine / Q000097:blood* / Q000737:chemistry; D005978:Glutathione / Q000097:blood* / Q000737:chemistry; D006801:Humans; D018384:Oxidative Stress; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D005978:Glutathione; D004837:Epinephrine