Characterization of extended-host-range pseudo-T-even bacteriophage Kpp95 isolated on Klebsiella pneumoniae.
Kpp95, isolated on Klebsiella pneumoniae, is a bacteriophage with the morphology of T4-type phages and is capable of rapid lysis of host cells. Its double-stranded genomic DNA (ca. 175 kb, estimated by pulsed-field gel electrophoresis) can be cut only by restriction endonucleases with a cleavage site flanked either by A and T or by T, as tested, suggesting that it contains the modified derivative(s) of G and/or C. Over 26 protein bands were visualized upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the virion proteins. N-terminal sequencing indicated that the most abundant band (46 kDa) is the major coat protein (gp23) which has been cleaved from a signal peptide likely with a length similar to that of T4. Phylogenetic analyses based on the sequences of the central region (263 amino acid residues) of gp23 and the full length of gp18 and gp19 placed Kpp95 among the pseudo-T-even subgroup, most closely related to the coliphage JS98. In addition to being able to lyse many extended-spectrum beta-lactamase strains of K. pneumoniae, Kpp95 can lyse Klebsiella oxytoca, Enterobacter agglomerans, and Serratia marcescens cells. Thus, Kpp95 deserves further studies for development as a component of a therapeutic cocktail, owing to its high efficiencies of host lysis plus extended host range.
Wu
Lii-Tzu
LT
Institute of Medical Biotechnology, Central Taiwan University of Science and Technology, Taichung 406, Taiwan, Republic of China. yhtseng@ctust.edu.tw.
Chang
Shu-Ying
SY
Yen
Ming-Ren
MR
Yang
Tsuey-Ching
TC
Tseng
Yi-Hsiung
YH
D001435:Bacteriophages / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology* / Q000648:ultrastructure; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004754:Enterobacter / Q000821:virology; D016679:Genome, Viral; D041121:Klebsiella oxytoca / Q000821:virology; D007711:Klebsiella pneumoniae / Q000821:virology*; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012706:Serratia marcescens; D013604:T-Phages / Q000235:genetics; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D014771:Virion / Q000648:ultrastructure
D004279:DNA, Viral; D014764:Viral Proteins; D004247:DNA; D004262:DNA Restriction Enzymes