[Hematoporphyrin derivative-mediated photodynamic therapy for human nasopharyngeal carcinoma: a comparative study with CNE2 and C666-1 cell lines in vitro].
OBJECTIVE
To investigate biological effect of hematoporphyrin derivative (HpD) photodynamic therapy (PDT) on in vitro cultured nasopharyngeal carcinoma (NPC) cell lines CNE2 and C666-1.


METHODS
CNE2 and C666-1 cells cultured in vitro were incubated in a medium containing HpD at different concentrations (0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, and 4.0 microg/ml) for 4 h followed by exposure to different light doses (2, 5, 10, and 20 J/cm2) using a diode laser at 630 nm with power density of 20 mW/cm2. After 24 h of incubation with HpD-PDT, the survival rate of CNE2 and C666-1 cells were analyzed by MTT assay.


RESULTS
HpD-PDT produced effective killing of CNE2 and C666-1 cells cultured in vitro, and the killing effects were positively correlated with HpD concentration and the irradiation dose. Exposure of CNE2 and C666-1 cells to irradiation dose of 20 J/cm2 resulted in the IC50 of 0.7 and 1.2 microg/ml, respectively (P<0.01). With the same HpD concentration and irradiation dose, the survival rate of C666-1 cells, however, was significantly higher than that of CNE2 cells (P<0.05).


CONCLUSIONS
HpD-PDT may result in effective killing of CNE2 and C666-1 cells cultured in vitro, although C666-1 cells are less sensitive to HpD-PDT than CNE2 cells.
Yang
Xiao-Min
XM
Oncotherapy Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. xiaomin-yang@163.com
Luo
Rong-Cheng
RC
Ma
Hong-Jing
HJ
Li
Li-Bo
LB
Ding
Xue-Mei
XM
Yan
Xiao
X
Lü
Cheng-Wei
CW
Zhou
Xiao-Ping
XP
D000970:Antineoplastic Agents / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D017324:Hematoporphyrin Derivative / Q000494:pharmacology*; D006414:Hematoporphyrin Photoradiation / Q000379:methods; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000473:pathology; D010778:Photochemotherapy / Q000379:methods; D017319:Photosensitizing Agents / Q000494:pharmacology*
D000970:Antineoplastic Agents; D017319:Photosensitizing Agents; D017324:Hematoporphyrin Derivative