Free-space fluorescence molecular tomography utilizing 360 degrees geometry projections.
Fluorescence tomography of diffuse media can yield optimal three-dimensional imaging when multiple projections over 360 degrees geometries are captured, compared with limited projection angle systems such as implementations in the slab geometry. We demonstrate how it is possible to perform noncontact, 360 degrees projection fluorescence tomography of mice using CCD-camera-based detection in free space, i.e., in the absence of matching fluids. This approach achieves high spatial sampling of photons propagating through tissue and yields a superior information content data set compared with fiber-based 360 degrees implementations. Reconstruction feasibility using 36 projections in 10 degrees steps is demonstrated in mice.
Deliolanis
Nikolaos
N
Laboratory for Biooptics and Molecular Imaging, Center for Molecular Imaging Research, Massachusetts General Hospital & Harvard Medical School, Charlestown, MA 02129, USA. ndeliolanis@partners.org
Lasser
Tobias
T
Hyde
Damon
D
Soubret
Antoine
A
Ripoll
Jorge
J
Ntziachristos
Vasilis
V
D000818:Animals; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation*; D021621:Imaging, Three-Dimensional / Q000295:instrumentation*; D051379:Mice; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D041622:Tomography, Optical / Q000295:instrumentation* / Q000379:methods