title: [Molecular relationship of Eurytrema coelmaticum inferred from 18S rRNA sequence].
abstract: OBJECTIVE
  To elucidate the taxonomic position of Eurytrema coelmaticum by using molecular technology.
  
  
  METHODS
  18S rRNA fragment was amplified from E. coelmaticum genomic DNA by specific conservative primers and sequenced. Homology and phylogenic tree of 18S rRNA sequences between E. coelmaticum and other Dicrocoeliidae trematodes were analyzed and constructed by DNAStar and MEGA3 respectively, and their evolutionary relationship was determined.
  
  
  RESULTS
  E. coelmaticum 18S rRNA sequence was with high homology to those from Dicrocoelium dendriticum, Lyperosomum collurionis and Brachylecithum lobatum. Among them, the diversity of E. coelmaticum from D. dendriticum was 2.42%, and that from L. collurionis was 1.75%; D. dendriticum and B. lobatum were closer in evolution only with 1.09% diversity.
  
  
  CONCLUSIONS
  For Dicrocoeliidae trematodes, classification based on 18S rRNA target is valid and the sequences are highly conservative. E. coelmaticum is evolutionarily closer to L. collurionis than to D. dendriticum and B. lobatum.
authors.affiliation: Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, CAAS, Lanzhou 730046, China.
authors.forename: Ya-dong
authors.initials: YD
authors.lastname: Zheng
authors.forename: Xue-nong
authors.initials: XN
authors.lastname: Luo
authors.forename: Cheng-hong
authors.initials: CH
authors.lastname: Shi
authors.forename: Rui-qian
authors.initials: RQ
authors.lastname: Zong
authors.forename: Zhi-zhong
authors.initials: ZZ
authors.lastname: Jing
authors.forename: Xue-peng
authors.initials: XP
authors.lastname: Cai
mesh_terms: D000818:Animals; D001483:Base Sequence; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D004012:Dicrocoeliidae / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D018510:RNA, Helminth / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D018509:DNA, Helminth; D018510:RNA, Helminth; D012337:RNA, Ribosomal, 18S