A fraction of Acorus calamus L. extract devoid of beta-asarone enhances adipocyte differentiation in 3T3-L1 cells.
The effects of fractions partitioned from the ethanol extract of Acorus calamus L. (AC) on adipocyte differentiation were investigated using cultured mouse 3T3-L1 preadipocytes. The degree of differentiation was evaluated by measuring the cellular triglycerides and protein expression of the glucose transporter GLUT4 in 3T3-L1 cells. The ethyl acetate fraction of the AC extract (ACE) was found to enhance adipocyte differentiation as did rosiglitazone. The results of further fractionation of ACE indicated that the active fraction does not consist of beta-asarone, which is a toxic component of this plant. This finding suggests that ACE has potential insulin-sensitizing activity like rosiglitazone, and may improve type 2 diabetes.
Wu
Hao-shu
HS
Department of Pharmacology and Toxicology, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.
Li
Ying-yin
YY
Weng
Lin-jia
LJ
Zhou
Chang-xin
CX
He
Qiao-jun
QJ
Lou
Yi-jia
YJ
D041721:3T3-L1 Cells; D000085:Acetates / Q000737:chemistry; D029023:Acorus / Q000737:chemistry*; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000081248:Allylbenzene Derivatives; D000818:Animals; D000840:Anisoles / Q000737:chemistry*; D002454:Cell Differentiation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D051275:Glucose Transporter Type 4 / Q000378:metabolism; D051379:Mice; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D000077154:Rosiglitazone; D045162:Thiazolidinediones / Q000494:pharmacology; D014280:Triglycerides / Q000378:metabolism
D000085:Acetates; D000081248:Allylbenzene Derivatives; D000840:Anisoles; D051275:Glucose Transporter Type 4; D010936:Plant Extracts; D045162:Thiazolidinediones; D014280:Triglycerides; C012195:asarone; D000077154:Rosiglitazone; C007650:ethyl acetate