title: Influence of transplantation of allogenic bone marrow mononuclear cells on the left ventricular remodeling of rat after acute myocardial infarction.
abstract: To probe into the influence of transplantation of allogenic bone marrow mononuclear cells (BM-MNCs) on the left ventricular remodeling of rat after acute myocardial infarction (AMI), 60 male Wistar rats were evenly divided into three groups at random: control group 1, control group 2 and transplantation group. In control group 1, chest was opened without ligation of coronary artery; in control group 2 and transplantation group, the left anterior descending branch of coronary artery was ligated to establish AMI model. Prepared culture medium and allogenic BM-MNCs suspension were respectively implanted the surrounding area of infarcted cardiac muscle via epicardium of control group 2 and transplantation group. Four weeks after the operation, the osteopontin gene (OPN mRNA, P<0.01), type I collagen (P<0.01) and angiotensin II (AngII, P<0.01) content in the left ventricular non-infarcted myocardium, and the Ang II density in blood plasma (P<0.05) of transplantation group and control group 2 were all significantly higher than that of control group 1. In the transplantation group, the myocardial OPN mRNA, type I collagen and Ang II content of non-infarcted zone in left ventricle, and the Ang II concentration in blood plasma were all significantly lower than those of control group 2 (P<0.05 for all). It is concluded that allogenic BM-MNCs transplantation may ease left ventricular remodeling after AMI by inhibiting the synthesis of type I collagen in the cardiac muscle and down-regulating the expression of Ang II and OPN gene.
authors.affiliation: Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. zhangrc200410@163.com
authors.forename: Ruicheng
authors.initials: R
authors.lastname: Zhang
authors.forename: Nianguo
authors.initials: N
authors.lastname: Dong
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Hou
authors.forename: Xian'en
authors.initials: X
authors.lastname: Fa
mesh_terms: D000804:Angiotensin II / Q000097:blood / Q000378:metabolism; D000818:Animals; D016026:Bone Marrow Transplantation; D024042:Collagen Type I / Q000096:biosynthesis; D004195:Disease Models, Animal; D007963:Leukocytes, Mononuclear / Q000637:transplantation; D008297:Male; D009203:Myocardial Infarction / Q000601:surgery*; D053495:Osteopontin / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020257:Ventricular Remodeling
chemical_list: D024042:Collagen Type I; C506897:Spp1 protein, rat; D053495:Osteopontin; D000804:Angiotensin II