Comparative cellular immune host response in acute vs healed lesions of cutaneous leishmaniasis.
BACKGROUND
Cutaneous leishmaniasis (CL) has become a major public health problem in Pakistan and research is now focused to characterize the host's immune response, its clinical correlation and subsequent implications in management in this disease. This study was done to evaluate cellular immune host response in patients with active and healed CL and its possible implications in prevention of disease in susceptibles.


METHODS
This cross sectional and comparative study was conducted in Armed Forces Institute of Pathology (AFIP) and Military Hospital (MH) Rawalpindi (1998-2000). 30 biopsies of active skin lesions and 15 biopsies from healed lesions, after processing, were studied for various immunophenotype cells by using monoclonal antibodies. Total and differential T cell counts were recorded in these skin tissues. Non parametric Kruskal-Wallis Test for one way ANOVA was used to compare the median cell counts between active, healed and normal skin and p-value < 0.05 was considered significant.


RESULTS
The total cell counts, CD3+ cells and CD57+ (NK) cells were found statistically different (p = < 0.001) when active forms of the disease were compared with healed lesions and normal skin tissue. The difference was not significant (p = > 0.05) on comparing healed lesions with normal skin tissue biopsies except in case of CD3+ cell counts (p = < 0.05). However, CD4+, CD8+ and CD19+ (Plasma cells) counts were never seen significant (p = > 0.05) on comparison.


CONCLUSIONS
NK cells and gamma delta cells seem to be responsible for limitation of the disease and elimination of the parasite from the lesion in cases of acute cutaneous leishmaniasis.
Rahman
Simeen Ber
SB
Dermatology Department, Military Hospital, Rawalpindi.
ul Bari
Arfan
A
D000293:Adolescent; D000328:Adult; D017252:CD3 Complex / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes; D018414:CD8-Positive T-Lymphocytes; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007694:Killer Cells, Natural; D016773:Leishmaniasis, Cutaneous / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D014945:Wound Healing / Q000502:physiology*
D017252:CD3 Complex