title: Confocal light absorption and scattering spectroscopic microscopy.
abstract: We have developed a novel optical method for observing submicrometer intracellular structures in living cells, which is called confocal light absorption and scattering spectroscopic (CLASS) microscopy. It combines confocal microscopy, a well-established high-resolution microscopic technique, with light-scattering spectroscopy. CLASS microscopy requires no exogenous labels and is capable of imaging and continuously monitoring individual viable cells, enabling the observation of cell and organelle functioning at scales of the order of 100 nm.
authors.affiliation: Biomedical Imaging and Spectroscopy Laboratory, Department of Medicine, Beth Israel Deaconess Medical Center, MA 02215, USA.
authors.forename: Hui
authors.initials: H
authors.lastname: Fang
authors.forename: Le
authors.initials: L
authors.lastname: Qiu
authors.forename: Edward
authors.initials: E
authors.lastname: Vitkin
authors.forename: Munir M
authors.initials: MM
authors.lastname: Zaman
authors.forename: Charlotte
authors.initials: C
authors.lastname: Andersson
authors.forename: Saira
authors.initials: S
authors.lastname: Salahuddin
authors.forename: Lauren M
authors.initials: LM
authors.lastname: Kimerer
authors.forename: Patsy B
authors.initials: PB
authors.lastname: Cipolloni
authors.forename: Mark D
authors.initials: MD
authors.lastname: Modell
authors.forename: Bradley S
authors.initials: BS
authors.lastname: Turner
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Keates
authors.forename: Irving
authors.initials: I
authors.lastname: Bigio
authors.forename: Irving
authors.initials: I
authors.lastname: Itzkan
authors.forename: Steven D
authors.initials: SD
authors.lastname: Freedman
authors.forename: Rama
authors.initials: R
authors.lastname: Bansil
authors.forename: Eugene B
authors.initials: EB
authors.lastname: Hanlon
authors.forename: Lev T
authors.initials: LT
authors.lastname: Perelman
mesh_terms: D004867:Equipment Design; D019544:Equipment Failure Analysis; D005240:Feasibility Studies; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis / Q000295:instrumentation* / Q000379:methods; D041623:Tomography, Optical Coherence / Q000295:instrumentation* / Q000379:methods