title: Multidrug resistance protein 1 is not associated to detergent-resistant membranes.
abstract: Multidrug resistance protein 1 (MRP1) is a member of the ATP-binding cassette superfamily. Using the energy provided by ATP hydrolysis, it transports a broad spectrum of substrates across the plasma membrane, including hormones, leukotriene C(4), bile salts, and anti-cancer drugs. Recent works have suggested that P-glycoprotein is associated to cholesterol and sphingolipid-rich membrane microdomains and that cholesterol upregulates its ATPase and drug transport activities. Confocal microscopy experiments and Triton X-100 extraction of detergent-resistant membranes provide evidence that MRP1 is not located in raft-like structures and that its activity is downregulated by cholesterol. The data are discussed in terms of cholesterol-protein interaction and topology.
authors.affiliation: Structure et Fonction des Membranes Biologiques, Centre de Biologie Structurale et de Bioinformatique, Université Libre de Bruxelles, Boulevard du Triomphe, B-1050 Brussels, Belgium.
authors.forename: Emilie
authors.initials: E
authors.lastname: Cerf
authors.forename: Régis
authors.initials: R
authors.lastname: Gasper
authors.forename: Jitendra D
authors.initials: JD
authors.lastname: Belani
authors.forename: Scott
authors.initials: S
authors.lastname: Rychnovsky
authors.forename: Xiu-Bao
authors.initials: XB
authors.lastname: Chang
authors.forename: Frédéric
authors.initials: F
authors.lastname: Buyse
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Ruysschaert
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000378:metabolism; D001692:Biological Transport; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D018613:Microscopy, Confocal; D017830:Octoxynol / Q000494:pharmacology*; D011485:Protein Binding
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D017830:Octoxynol; D000251:Adenosine Triphosphatases