title: Reliability of the interval death rate analysis for estimating the time course of the motoneurone afterhyperpolarization in humans.
abstract: The reliability of the afterhyperpolarization (AHP) time course, as estimated by the interval death rate (IDR) analysis was evaluated both within and between investigators. The IDR analysis uses the firing history of a single motor unit train at low tonic firing rates to calculate an estimate of the AHP time course [Matthews PB. Relationship of firing intervals of human motor units to the trajectory of post-spike after-hyperpolarization and synaptic noise. J Physiol 1996;492:597-628]. Single motor unit trains were collected from the tibialis anterior (TA) to determine intra-rater reliability (within investigator). Data from the first dorsal interosseus (FDI), collected in a previous investigation [Gossen ER, Ivanova TD, Garland SJ. The time course of the motoneurone afterhyperpolarization is related to motor unit twitch speed in human skeletal muscle. J Physiol 2003;552:657-64], were used to examine the inter-rater reliability (between investigators). The lead author was blinded to the original time constants and file identities for the re-analysis. The intra-rater reliability of the AHP time constant in the TA data was high (r(2)=0.88; p<0.001; ICC=0.91). The inter-rater reliability for the FDI data was also strong (r(2)=0.92; p<0.001; ICC=0.95). The standard error of measurement was 0.61 ms for the TA and 0.55 ms for FDI. It is concluded that the interval death rate analysis is a reliable tool for estimating the AHP time course with experienced investigators.
authors.affiliation: Graduate Program in Neuroscience, University of Western Ontario, London, Ontario, Canada. cmacdone@uwo.ca
authors.forename: Christopher William
authors.initials: CW
authors.lastname: MacDonell
authors.forename: Tanya Dimitrova
authors.initials: TD
authors.lastname: Ivanova
authors.forename: S Jayne
authors.initials: SJ
authors.lastname: Garland
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000328:Adult; D016923:Cell Death; D005260:Female; D006801:Humans; D007700:Kinetics; D008297:Male; D009046:Motor Neurons / Q000166:cytology* / Q000502:physiology*; D015588:Observer Variation; D012016:Reference Values; D012044:Regression Analysis; D015203:Reproducibility of Results