Distribution of supplemental Escherichia coli AppA2 phytase activity in digesta of various gastrointestinal segments of young pigs.
The objective of this study was to determine the functional location and disappearance of activity of a supplemental Escherichia coli AppA2 phytase and its impact on digesta P and Ca concentrations in the gastrointestinal tract of pigs. In Exp. 1, 18 pigs (8.3 +/- 0.2 kg of BW) were allotted to 3 groups (n = 6 each) and fed a low-P (0.4%) corn-soybean meal, basal diet (BD), BD + phytase [500 units (U)/kg of feed], or BD + inorganic P (iP, 0.1%) for 4 wk. In Exp. 2, 30 pigs (14.5 +/- 0.2 kg of BW) were allotted to 3 groups (n = 10 each) and fed BD, BD + 500 U of phytase/kg of feed, or BD + 2,000 U of phytase/kg of feed for 2 wk. Five or six pigs from each treatment group were killed at the end of both experiments to assay for digesta phytase activity and soluble P concentration in 6 segments of the digestive tract and digesta total P and Ca concentrations in stomach and colon. Compared with pigs fed BD, pigs fed BD + 500 U of phytase/kg of feed in Exp. 1 and BD + 2,000 U of phytase/kg of feed in Exp. 2 had greater (P < 0.05) phytase activities in the digesta of the stomach and upper jejunum (2 m aborally from the duodenum). No phytase activity was detected in the digesta of the lower jejunum (2.12 m cranial to the ileocecal junction) or ileum from any of the treatment groups in either trial. Concentrations of digesta-soluble P peaked in the upper jejunum of pigs fed BD in Exp. 1 and 2, but showed gradual decreases between the stomach and the upper jejunum of pigs fed BD + phytase or BD + iP. In both experiments, pigs fed only BD had greater (P < 0.05) colonic digesta phytase activity and soluble P concentrations than those fed phytase. In Exp. 2, total colonic digesta P or Ca concentrations, or both, of pigs displayed a phytase-dose-dependent reduction (P < 0.05). In conclusion, supplemental dietary AppA2 mainly functioned in the stomach and was associated with a reduced phytase activity in colonic digesta of weanling pigs.
Pagano
A R
AR
Department of Animal Science, Cornell University, Ithaca, NY 14853, USA.
Roneker
K R
KR
Lei
X G
XG
D010832:6-Phytase / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D000135:Acid Phosphatase / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002118:Calcium / Q000737:chemistry / Q000378:metabolism; D003106:Colon / Q000187:drug effects / Q000201:enzymology; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements; D004386:Duodenum / Q000187:drug effects / Q000201:enzymology; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D005766:Gastrointestinal Contents / Q000737:chemistry*; D007082:Ileum / Q000201:enzymology; D007583:Jejunum / Q000201:enzymology; D009097:Multienzyme Complexes / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D010758:Phosphorus / Q000737:chemistry / Q000378:metabolism; D013270:Stomach / Q000201:enzymology; D013552:Swine / Q000033:anatomy & histology / Q000378:metabolism*
D029968:Escherichia coli Proteins; D009097:Multienzyme Complexes; D010758:Phosphorus; D000135:Acid Phosphatase; D010832:6-Phytase; C118716:AppA2 protein, E coli; D002118:Calcium