title: A novel microarray approach reveals new tissue-specific signatures of known and predicted mammalian microRNAs.
abstract: Microarrays to examine the global expression levels of microRNAs (miRNAs) in a systematic in-parallel manner have become important tools to help unravel the functions of miRNAs and to understand their roles in RNA-based regulation and their implications in human diseases. We have established a novel miRNA-specific microarray platform that enables the simultaneous expression analysis of both known and predicted miRNAs obtained from human or mouse origin. Chemically modified 2'-O-(2-methoxyethyl)-(MOE) oligoribonucleotide probes were arrayed onto Evanescent Resonance (ER) microchips by robotic spotting. Supplementing the complementary probes against miRNAs with carefully designed mismatch controls allowed for accurate sequence-specific determination of miRNA expression profiles obtained from a panel of mouse tissues. This revealed new expression signatures of known miRNAs as well as of novel miRNAs previously predicted using bioinformatic methods. Systematic confirmation of the array data with northern blotting and, in particular, real-time PCR suggests that the described microarray platform is a powerful tool to analyze miRNA expression patterns with rapid throughput and high fidelity.
authors.affiliation: Novartis Institutes for BioMedical Research, Genome and Proteome Sciences, Basel, Switzerland.
authors.forename: Iwan
authors.initials: I
authors.lastname: Beuvink
authors.forename: Fabrice A
authors.initials: FA
authors.lastname: Kolb
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Budach
authors.forename: Arlette
authors.initials: A
authors.lastname: Garnier
authors.forename: Joerg
authors.initials: J
authors.lastname: Lange
authors.forename: Francois
authors.initials: F
authors.lastname: Natt
authors.forename: Uwe
authors.initials: U
authors.lastname: Dengler
authors.forename: Jonathan
authors.initials: J
authors.lastname: Hall
authors.forename: Witold
authors.initials: W
authors.lastname: Filipowicz
authors.forename: Jan
authors.initials: J
authors.lastname: Weiler
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes / Q000737:chemistry; D012313:RNA / Q000737:chemistry; D014018:Tissue Distribution
chemical_list: D035683:MicroRNAs; D015345:Oligonucleotide Probes; D012313:RNA