title: Adherence of enterohemorrhagic Escherichia coli O157, O26, and O111 strains to bovine intestinal explants ex vivo.
abstract: We used bovine intestinal organ culture to study infection by enterohemorrhagic Escherichia coli serogroups O157, O26, and O111. We show colonization and attaching and effacing lesion formation on explants derived from the ileum, colon, and rectum. Intimin and Tir were detected at the sites of adherent bacteria; Tir was essential for colonization.
authors.affiliation: Division of Cell and Molecular Biology, Imperial College London, London, UK.
authors.forename: Francis
authors.initials: F
authors.lastname: Girard
authors.forename: Francis
authors.initials: F
authors.lastname: Dziva
authors.forename: Pauline
authors.initials: P
authors.lastname: van Diemen
authors.forename: Alan D
authors.initials: AD
authors.lastname: Phillips
authors.forename: Mark P
authors.initials: MP
authors.lastname: Stevens
authors.forename: Gad
authors.initials: G
authors.lastname: Frankel
mesh_terms: D018829:Adhesins, Bacterial / Q000378:metabolism; D000818:Animals; D001422:Bacterial Adhesion; D002417:Cattle; D054324:Enterohemorrhagic Escherichia coli / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000378:metabolism; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000382:microbiology*; D011956:Receptors, Cell Surface / Q000378:metabolism; D013045:Species Specificity; D046509:Tissue Culture Techniques
chemical_list: D018829:Adhesins, Bacterial; D029968:Escherichia coli Proteins; D011956:Receptors, Cell Surface; C109287:Tir protein, E coli; C073819:eaeA protein, E coli