[A study on the mechanism of inducing apoptosis of Tca8113 cells by means of ultrasound hyperthermia].
OBJECTIVE
To investigate the mechanism of apoptosis in Tca8113 cells induced by ultrasound hyperthermia by detecting changes in related index.


METHODS
Tca8113 cells were treated in vitro by ultrasound hyperthermia in different heating temperatures (38 degrees C to 44 degrees C,10 minutes) and heating times (42 degrees C,10 to 60 minutes), and then the dynamic changes of early apoptosis and secondary necrosis treated by 42 degrees C for 10 minutes were assayed to detect deltapsim and Caspase-3 levels of different groups by flow cytometry (FCM). The means of each group were compared by ANOVA with SAS6.12 software package.


RESULTS
After heated by ultrasound in 42 degrees C for 10 minutes, the early apoptosis of Tca8113 was detected. The apoptosis index reached its highest level at the 6th to 8th hour, then decreased rapidly and maintained in a lower level after 12 hours. The level of secondary necrosis increased with the level of early apoptosis, but kept in a higher level until the 10th hour, the level of secondary necrosis correlated with that of the early apoptosis (r = 0.7909, P = 0.0064). The fraction of cells with low mitochondria membrane potential and increased activity of Caspase-3 were detected either in the heating-temperature grads group or in the heating-time grads group, which showed significant relationship between thetwo apoptosis related index (r = 0.89189, P = 0.0029 in the heating-temperature grads group; r = 0.9679, P = 0.0003 in the heating-time grads group).


CONCLUSIONS
Tca8113 cells developed apoptosis after heated by ultrasound hyperthermia along with deltapsim level decreasing and Caspase-3 activity increasing. Ultrasound hyperthermia induces apoptosis of Tca8113 cells by the mitochondrum-Caspase pathway.
Ren
Guo-Xin
GX
Biomedical Instrument, College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. renguoxin@sjtu.edu.cn
Guo
Wei
W
Ye
Dong-Xia
DX
Shen
Guo-Feng
GF
Bai
Jing-Feng
JF
Li
Sheng-Jiao
SJ
Chen
Ya-Zhu
YZ
D017209:Apoptosis; D002294:Carcinoma, Squamous Cell; D053148:Caspase 3; D005434:Flow Cytometry; D006358:Hot Temperature; D006801:Humans; D008928:Mitochondria; D014062:Tongue Neoplasms; D014464:Ultrasonic Therapy
D053148:Caspase 3