title: A putative gene cluster for aminoarabinose biosynthesis is essential for Burkholderia cenocepacia viability.
abstract: Using a conditional mutagenesis strategy we demonstrate here that a gene cluster encoding putative aminoarabinose (Ara4N) biosynthesis enzymes is essential for the viability of Burkholderia cenocepacia. Loss of viability is associated with dramatic changes in bacterial cell morphology and ultrastructure, increased permeability to propidium iodide, and sensitivity to sodium dodecyl sulfate, suggesting a general cell envelope defect caused by the lack of Ara4N.
authors.affiliation: Department of Microbiology and Immunology, Infectious Diseases Research Group, Siebens-Drake Medical Research Institute, University of Western Ontario, London, Ontario, Canada.
authors.forename: Ximena P
authors.initials: XP
authors.lastname: Ortega
authors.forename: Silvia T
authors.initials: ST
authors.lastname: Cardona
authors.forename: Alan R
authors.initials: AR
authors.lastname: Brown
authors.forename: Slade A
authors.initials: SA
authors.lastname: Loutet
authors.forename: Ronald S
authors.initials: RS
authors.lastname: Flannagan
authors.forename: Dominic J
authors.initials: DJ
authors.lastname: Campopiano
authors.forename: John R W
authors.initials: JR
authors.lastname: Govan
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Valvano
mesh_terms: D001089:Arabinose / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000235:genetics; D042602:Burkholderia cepacia complex / Q000166:cytology / Q000235:genetics* / Q000502:physiology*; D005798:Genes, Bacterial; D020043:Genes, Essential; D050296:Microbial Viability / Q000235:genetics*; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D005810:Multigene Family; D016254:Mutagenesis, Insertional; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic
chemical_list: C016982:aminoarabinose; D001089:Arabinose