title: Genetic instability induced by low doses of x-rays in hamster cells.
abstract: OBJECTIVE
  Genomic instability involves time delayed events and can be manifested as elevated rates of heritable changes in the progeny of irradiated cells. To study the induction of chromosomal instability by very low doses of radiation Chinese Hamster Ovary (CHO) cells were exposed to 10-50 milisieverts (mSv) (approximately 10-50 miligrays (mGy)) of x-rays.
  
  
  METHODS
  Control and irradiated cell populations were assayed for chromosomal aberrations and assessed using a micronucleus test and anaphase-telophase analysis at the first cell division post-irradiation and at every four population doublings thereafter up to 16 population doublings post-irradiation.
  
  
  RESULTS
  Frequencies of micronuclei, anaphase-telophase alterations and chromosomal aberrations were increased when the cells were analysed immediately after x-ray exposure. Micronuclei and anaphase-telophase alterations showed significantly increased frequencies when they were analysed at 12 and 16 population doublings after exposure to 50 mSv. Chromosomal aberrations increased significantly at 12 and 16 population doublings after exposure to 10 mSv and 50 mSv.
  
  
  CONCLUSIONS
  Our results are consistent with the presence of a phenomenon by which the initial DNA damage in the surviving cells is memorized. Micronuclei and achromatic lessions were the main cytogenetic damage observed in cells exposed to very low doses of x-rays, indicating that these low doses are able to induce genetic instability.
authors.affiliation: Centro de Investigaciones en Genética Básica y Aplicada (CIGEBA), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Argentina. aseoane@fcv.unlp.edu.ar
authors.forename: A
authors.initials: A
authors.lastname: Seoane
authors.forename: A
authors.initials: A
authors.lastname: Güerci
authors.forename: F
authors.initials: F
authors.lastname: Dulout
mesh_terms: D000818:Animals; D016466:CHO Cells / Q000528:radiation effects; D002869:Chromosome Aberrations / Q000528:radiation effects*; D006224:Cricetinae; D003412:Cricetulus; D020732:Cytogenetic Analysis / Q000379:methods; D004307:Dose-Response Relationship, Radiation; D005260:Female; D010053:Ovary / Q000166:cytology / Q000528:radiation effects*; D014965:X-Rays