High mobility group HMGI(Y) protein expression in head and neck squamous cell carcinoma.
CONCLUSIONS
We conclude that increased expression level of the high mobility group I (HMGI(Y)) is closely associated with malignant transformation in head and neck squamous cell carcinomas (HNSCCs), and the measurement of HMGI(Y) levels in HNSCCs may be useful as a prognostic marker.


OBJECTIVE
To investigate whether HMGI overexpression is observed in HNSCCs, and its value as a prognostic marker in HNSCCs.


METHODS
HMGI(Y) expression was determined at the protein level by immunohistochemisty using a HMGI(Y)-specific antibody and RT-PCR in 10 surgically resected specimens of non-neoplastic tissue (normal palatal tissue) and 40 HNSCCs. We also evaluated the association of HMGI(Y) overexpression within clinicopathologic parameters, i.e. clinical stage, pathologic grade, status of cervical lymph node metastasis, recurrence rate.


RESULTS
Expression of HMGI(Y) by immunohistochemical staining was observed in 35 of 40 (87.5%) HNSCC samples, whereas normal mucosa and/or the mucosa adjacent to the tumor tissue showed negative or weakly positive staining (p<0.05). Semi-quantification of HMGI(Y) by RT-PCR was 2.98+/-2.24 in cancer and 0.47+/-0.25 in normal tissue (p<0.001). High expression of HMGI(Y) was observed in recurrent cases, compared with non-recurrent cases (p<0.05). However, no significant correlation was observed between the levels of HMGI(Y) expression and other clinical factors such as clinical stage, pathologic grade, and status of cervical lymph node metastasis.
Rho
Young Soo
YS
Department of Otorhinolaryngology, Head and Neck Surgery, College of Medicine, Hallym University, Seoul, South Korea. ys20805@chol.com
Lim
Young Chang
YC
Park
Il Seok
IS
Kim
Jin Hwan
JH
Ahn
Hwoe Young
HY
Cho
Seong Jin
SJ
Shin
Hyung Sik
HS
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000906:Antibodies / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D005260:Female; D025741:HMGA1a Protein / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009092:Mucous Membrane / Q000276:immunology / Q000378:metabolism; D009367:Neoplasm Staging; D011379:Prognosis; D012313:RNA / Q000235:genetics / Q000276:immunology / Q000378:metabolism
D000906:Antibodies; D017931:DNA Primers; D025741:HMGA1a Protein; D012313:RNA