title: [Conservation of the MC domains in eukaryotic termination factor eRF3].
abstract: Eukaryotic translation termination employs two protein factors, eRF1 and eRF3. Proteins of the eRF3 family each consist of three domains. The N and M domains vary in different species, while the C domains are highly homologous. The MC domains of Homo sapiens eRF3a (hGSPT I), Xenopus laevis eRF3 (XSup35), and Mus musculus eRF3a (mGSPTI) and eRF3b (mGSPT2) were found to compensate for the sup35-21(ts) temperature-sensitive mutation and lethal disruption of the SUP35 gene in yeast Saccharomyces cerevisiae. At the same time, strains containing the MC domains of the eRF3 proteins from different species differed in growth rate and the efficiency of translation termination.
authors.forename: G A
authors.initials: GA
authors.lastname: Zhuravleva
authors.forename: O M
authors.initials: OM
authors.lastname: Zemlianko
authors.forename: C
authors.initials: C
authors.lastname: Le Goff
authors.forename: A V
authors.initials: AV
authors.lastname: Petrova
authors.forename: M
authors.initials: M
authors.lastname: Philippe
authors.forename: S G
authors.initials: SG
authors.lastname: Inge-Vechtomov
mesh_terms: D000818:Animals; D018388:Codon, Terminator; D006801:Humans; D051379:Mice; D009154:Mutation; D010454:Peptide Termination Factors / Q000235:genetics* / Q000378:metabolism; D011328:Prions / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism; D014982:Xenopus laevis
chemical_list: D018388:Codon, Terminator; D010454:Peptide Termination Factors; D011328:Prions; C068068:SUP35 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D029867:Xenopus Proteins; C087971:peptide-chain-release factor 3