title: Trehalose lyophilized platelets for wound healing.
abstract: Fresh platelet preparations are utilized to treat a wide variety of wounds, although storage limitations and mixed results have hampered their clinical use. We hypothesized that concentrated lyophilized and reconstituted platelet preparations, preserved with trehalose, maintain and possibly enhance fresh platelets' ability to improve wound healing. We studied the ability of a single dose of trehalose lyophilized and reconstituted platelets to enhance wound healing when topically applied on full-thickness wounds in the genetically diabetic mouse. We compared these results with the application of multiple doses of fresh platelet preparations and trehalose lyophilized and reconstituted platelets as well as multiple doses of vascular endothelial growth factor (VEGF) and wounds left untreated. Trehalose lyophilized and reconstituted platelets, in single and multiple applications, multiple applications of fresh platelets and multiple applications of VEGF increased granulation tissue deposition, vascularity, and proliferation when compared with untreated wounds, as assessed by histology and immunohistochemistry. Wounds treated with multiple doses of VEGF and a single dose of freeze-dried platelets reached 90% closure faster than wounds left untreated. A single administration of trehalose lyophilized and reconstituted platelet preparations enhanced diabetic wound healing, therefore representing a promising strategy for the treatment of nonhealing wounds.
authors.affiliation: Division of Plastic Surgery, Brigham and Women's Hospital, Boston, MA 02115, USA.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Pietramaggiori
authors.forename: Arja
authors.initials: A
authors.lastname: Kaipainen
authors.forename: David
authors.initials: D
authors.lastname: Ho
authors.forename: Cindy
authors.initials: C
authors.lastname: Orser
authors.forename: Walter
authors.initials: W
authors.lastname: Pebley
authors.forename: Alan
authors.initials: A
authors.lastname: Rudolph
authors.forename: Dennis P
authors.initials: DP
authors.lastname: Orgill
mesh_terms: D000287:Administration, Topical; D000818:Animals; D001792:Blood Platelets / Q000502:physiology*; D049109:Cell Proliferation; D004797:Enzyme-Linked Immunosorbent Assay; D005612:Freeze Drying; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D023421:Models, Animal; D010949:Plasma; D017713:Platelet Transfusion; D014199:Trehalose / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology; D014945:Wound Healing / Q000502:physiology*
chemical_list: D042461:Vascular Endothelial Growth Factor A; D014199:Trehalose