Treatment with beta2-adrenoceptor agonist in vivo induces human clock gene, Per1, mRNA expression in peripheral blood.
This study examined whether in vivo exposure to a beta2-adrenoceptor agonist, tulobuterol, induces human Period1 (hPer1) mRNA expression in cells from peripheral whole blood. In one experiment, oral tulobuterol was administered to five healthy volunteers at 22:00 h, while in another, a transdermally tulobuterol patch was applied to the same five subjects at 20:00 h. In each experiment, serum tulobuterol concentrations were measured at four time points, and total RNA was isolated from peripheral blood cells for determinations of hPer1 mRNA expression by real-time polymerase chain reaction. Both the tulobuterol tablet and the transdermal patch increased hPer1 mRNA expression, suggesting that analyses of human peripheral blood cells could reliably represent peripheral clock gene mRNA expression in vivo.
Burioka
Naoto
N
Division of Medical Oncology and Molecular Respirology, Tottori University, Nishimachi, Yonago, Japan. burioka@grape.med.tottori-u.ac.jp
Takata
Miyako
M
Endo
Masahiro
M
Miyata
Masanori
M
Takeda
Kenichi
K
Chikumi
Hiroki
H
Tomita
Katsuyuki
K
Fukuoka
Yasushi
Y
Nakazaki
Hirofumi
H
Sano
Hiroyuki
H
Shimizu
Eiji
E
D000318:Adrenergic beta-Agonists / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D000328:Adult; D018797:Cell Cycle Proteins / Q000097:blood* / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D008297:Male; D056950:Period Circadian Proteins; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics; D013726:Terbutaline / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood / Q000494:pharmacology
D000318:Adrenergic beta-Agonists; D018797:Cell Cycle Proteins; C539022:PER1 protein, human; D056950:Period Circadian Proteins; D012333:RNA, Messenger; C010809:tulobuterol; D013726:Terbutaline