title: [Application of multiplex allele-specific PCR for authentication of Panax ginseng and P. quinquefolius].
abstract: OBJECTIVE
  Searching and identifying SNP in Panax species and using multiplex allele-specific PCR (MAS-PCR) to authenticate P. ginseng and P. quenquefolium.
  
  
  METHODS
  Based on genbank database of Panax species, using DNAMAN to align the sequences, identify SNP of P. ginseng and P. quenquefolium. Design allele-specific primers for P. ginseng and P. quenquefolium, optimize the PCR reaction system including the usage amount of Taq, dNTP, primer, etc. Optimized system was performed with the total DNA of 20 different sources of P. ginseng and P. quenquefolium.
  
  
  RESULTS
  When the annealing temperature was 66 'C, the template DNA of P. ginseng could be amplified 249 bp band whereas P. quenquefolium amplified 1 049 bp band.
  
  
  CONCLUSIONS
  The MAS-PCR have the advantages of highly specific, good reproducibility and could be identify P. ginseng and P. quenquefolium in the same PCR tube. It was a potential method to use in the molecular identification of other meteria medica.
authors.affiliation: Institute of Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Guang-hong
authors.initials: GH
authors.lastname: Cui
authors.forename: Xiao-jing
authors.initials: XJ
authors.lastname: Tang
authors.forename: Lu-qi
authors.initials: LQ
authors.lastname: Huang
mesh_terms: D000483:Alleles; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers; D005894:Panax / Q000145:classification / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics; D010946:Plants, Medicinal / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide; D015203:Reproducibility of Results; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers