Effect of triphenyldioxane on phase I xenobiotic metabolism enzymes in the liver of rats and rabbits.
We studied the effect of triphenyldioxane on phase I xenobiotic metabolism enzymes in the liver of rats and rabbits. Total cytochrome P450 content, protein concentration, and catalytic activity of CYP2B, CYP3A, and CYP2C isoforms were measured. Triphenyldioxane significantly increases specific activity of CYP2B and CYP2C in the liver of rats and rabbits, respectively. Immunoblotting analysis of microsomal enzymes in the liver of animals showed that the increase in specific activity of CYP is related to high content of apoenzymes. We showed for the first time that rats and rabbits are characterized by interspecies differences in the induction of cytochrome P450 isoforms under the influence of triphenyldioxane.
Pustyl'nyak
V O
VO
Laboratory for Molecular Mechanisms of Carcinogenesis, Institute of Molecular Biology and Biophysics, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk. pustylnyak@ngs.ru
Cirulli
V
V
Jervazi
P J
PJ
Yaroslavtsev
D
D
Gulyaeva
L F
LF
Lyakhovich
V V
VV
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D004146:Dioxanes / Q000633:toxicity*; D004790:Enzyme Induction / Q000187:drug effects; D005557:Formaldehyde / Q000032:analysis; D015151:Immunoblotting; D008099:Liver / Q000187:drug effects* / Q000201:enzymology*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D020033:Protein Isoforms / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D013045:Species Specificity; D013730:Terphenyl Compounds / Q000633:toxicity*
D004146:Dioxanes; D020033:Protein Isoforms; D013730:Terphenyl Compounds; D005557:Formaldehyde; D003577:Cytochrome P-450 Enzyme System