title: Avian mortality surveillance for West Nile virus in Colorado.
abstract: We tested 1,549 avian carcasses of 104 species to identify targets for West Nile virus (WNV) surveillance in Colorado, determine species affected by WNV, compare virus isolation versus RNA detection applied to hearts and oral swabs from carcasses, and compare the VecTest WNV Antigen Assay (VecTest) to standard assays. Forty-two species tested positive. From June to September 2003, 86% of corvids, 34% of non-corvid passerines, and 37% of raptors tested positive. We developed the Target Species Index, which identified American crows as the most important avian indicator species. However, testing multiple species maximizes detection, which may be important early and late in the transmission season. This index may benefit surveillance for other zoonotic pathogens, such as highly pathogenic avian influenza H5N1 virus. VecTest using oral swabs was most sensitive for American crow, black-billed magpie, house finch, house sparrow, and American kestrel. Wildlife rehabilitation centers should be recruited to enhance WNV surveillance.
authors.affiliation: Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA. nnemeth@colostate.edu
authors.forename: Nicole M
authors.initials: NM
authors.lastname: Nemeth
authors.forename: Susan
authors.initials: S
authors.lastname: Beckett
authors.forename: Eric
authors.initials: E
authors.lastname: Edwards
authors.forename: Kaci
authors.initials: K
authors.lastname: Klenk
authors.forename: Nicholas
authors.initials: N
authors.lastname: Komar
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001715:Bird Diseases / Q000175:diagnosis / Q000401:mortality*; D001717:Birds / Q000821:virology*; D014901:West Nile Fever / Q000175:diagnosis / Q000401:mortality / Q000662:veterinary*; D014902:West Nile virus / Q000302:isolation & purification*
chemical_list: D000956:Antigens, Viral