Estimating the number of coding mutations in genotypic and phenotypic driven N-ethyl-N-nitrosourea (ENU) screens: revisited.
We recently described methods for estimating the number of N-ethyl-N-nitrosourea (ENU)-induced coding mutations in phenotypic and genotypic screens. In this article we revisit these methods, clarifying their application. In particular, we focus on the difference between unconditional and conditional probabilities. We also introduce a website to assist investigators in the application of these equations ( http://www.well.ox.ac.uk/enuMutRat ).
Keays
David A
DA
Psychiatric Genetics Laboratory, Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford, OX3 7BN, United Kingdom. david.keays@physiol.ox.ac.uk
Clark
Taane G
TG
Campbell
Thomas G
TG
Broxholme
John
J
Valdar
William
W
D005038:Ethylnitrosourea / Q000494:pharmacology*; D005820:Genetic Testing / Q000379:methods*; D005838:Genotype; D016296:Mutagenesis; D009154:Mutation; D010641:Phenotype; D011336:Probability
D005038:Ethylnitrosourea