[Apoptotic effect of the combined treatment of engineered antibody with paclitaxel on BT474 cells].
OBJECTIVE
To explore the apoptotic effect of the combined treatment of anti-p185(c-erbB-2/neu) engineered antibody with paclitaxel on p185-overexpressing human malignant breast cancer cell lines BT474 and to study its emerging mechanism.


METHODS
The prohibitory effect of engineeded antibody plus paclitaxel on BT474 cells was assessed by MTS assay. The number of apoptotic cells was detected by Annexin V-FITC/PI. DNA content and cell cycle distribution were determined by FCM; DNA-binding activity of NF-kappaB was demonstrated by EMSA.


RESULTS
Anti-p185(c-erbB-2/neu) engineered antibody plus paclitaxel resulted in synergistic effect on proliferative inhibiton of BT474 cells, which was mediated via apoptotic induction and caused cell cycle to arrest at G1 phase remarkably. Furthermore, the combined treatment of the engineered antibody with paclitaxel effectively suppressed the activation of NF-kappaB in BT474 cells.


CONCLUSIONS
The combined treatment of anti-p185(c-erbB-2/neu) engineered antibody with paclitaxel rendered p185-overexpressing human malignant breast cancer cells BT474 more susceptible to paclitaxel-induced apoptosis by the effective suppression of NF-kappaB activation.
Yin
Hui-Jing
HJ
Lab of Molecular Genetics, School of Life Science, Anhui Agricultural University, Hefei, China. yhj_an@yahoo.com.cn
Cheng
Lian-Sheng
LS
Duan
Jia-Long
JL
D000906:Antibodies / Q000276:immunology / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D024202:Electrophoretic Mobility Shift Assay; D005434:Flow Cytometry; D016193:G1 Phase / Q000187:drug effects; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism; D017239:Paclitaxel / Q000494:pharmacology*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000276:immunology
D000906:Antibodies; D000972:Antineoplastic Agents, Phytogenic; D016328:NF-kappa B; D017239:Paclitaxel; D018719:Erb-b2 Receptor Tyrosine Kinases