Visualization of hepatobiliary excretory function by intravital multiphoton microscopy.
Intravital imaging of hepatobiliary excretion is vital for elucidating liver metabolism. In this work, we describe a novel method to observe the intravital dynamics of the uptake, processing, and excretion of an organic anion, 6-carboxyfluorescein diacetate (6-CFDA) in the hepatobiliary system. This is achieved by the use of multiphoton microscopy and an intravital hepatic imaging chamber. The high-quality images show sequential uptake and processing of 6-CFDA from the hepatocytes and the subsequent excretion into bile canaliculi within approximately 50 min. This is a promising technique to study intravital hepatic physiology and metabolism.
Liu
Yuan
Y
National Taiwan University, Department of Physics, Taipei 106, Taiwan.
Chen
Hsiao-Ching
HC
Yang
Shu-Mei
SM
Sun
Tzu-Lin
TL
Lo
Wen
W
Chiou
Ling-Ling
LL
Huang
Guan Tarn
GT
Dong
Chen-Yuan
CY
Lee
Hsuan-Shu
HS
D000818:Animals; D001659:Biliary Tract / Q000378:metabolism*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005452:Fluoresceins / Q000378:metabolism*; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D005452:Fluoresceins; C093616:6-carboxyfluorescein diacetate