Lung cancer-associated SNP at the beginning of mouse k-ras gene intron 2 is essential for transcription factor binding.
The C to T substitution in position 311 n. p. of K-ras gene intron 2 in mice resistant to lung cancer (M. spretus) attenuates NF-Y transcription factor binding site in comparison with sensitive ICR mice (M. musculus). Appreciable differences between ICR and M. spretus in general pattern of binding of nuclear proteins to K-ras gene DNA within 248-332 n. p. fragment are demonstrated.
Gorshkova
E V
EV
Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk. schly@mail.ru
Kaledin
V I
VI
Kobzev
V F
VF
Merkulova
T I
TI
D000818:Animals; D001665:Binding Sites / Q000235:genetics; D023081:CCAAT-Binding Factor / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D011905:Genes, ras / Q000235:genetics*; D007438:Introns / Q000235:genetics*; D008175:Lung Neoplasms / Q000235:genetics*; D051379:Mice / Q000235:genetics*; D008813:Mice, Inbred ICR; D009687:Nuclear Proteins / Q000378:metabolism; D009841:Oligonucleotides; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
D023081:CCAAT-Binding Factor; D009687:Nuclear Proteins; D009841:Oligonucleotides