title: Identification of differentially expressed genes in senescence-accelerated mouse testes by suppression subtractive hybridization analysis.
abstract: Senescence-accelerated mouse (SAM) strains constitute a model of accelerated senescence coupled with a short lifespan and the early development of various age-related disorders. To identify differential gene expression in testes between senescence-accelerated SAMP1 and control SAMR1 mice, we performed suppression subtractive hybridization. We observed that the expression of three genes related to cell proliferation (myosin regulatory light chain B, aldolase 1A isoform, and cytochrome c oxidase subunit VIc) were upregulated and four genes implicated in spermatogenesis were downregulated in SAMP1 mice. Asb-8, a member of ankyrin repeat-containing proteins, was abundantly expressed in the testes and downregulated in SAMP1. The other three downregulated genes (germ cell-specific gene 1, T-complex polypeptide 1b, and activator of cAMP responsive element modulator in testis) have been reported to regulate late-stage spermatogenesis. These gene expression profiles might explain the findings of early testicular maturation and rapid decline in the ability to produce spermatozoa with advancing age in SAMP1 mice.
authors.affiliation: Department of Investigative Pathology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, 852-8523, Japan. takuya@net.nagasaki-u.ac.jp
authors.forename: Takuya
authors.initials: T
authors.lastname: Chiba
authors.forename: Junjie
authors.initials: J
authors.lastname: Yao
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Higami
authors.forename: Isao
authors.initials: I
authors.lastname: Shimokawa
authors.forename: Masanori
authors.initials: M
authors.lastname: Hosokawa
authors.forename: Keiichi
authors.initials: K
authors.lastname: Higuchi
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D003576:Electron Transport Complex IV / Q000235:genetics*; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D008297:Male; D051379:Mice; D018994:Myosin Light Chains / Q000235:genetics; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013091:Spermatogenesis; D013737:Testis / Q000254:growth & development / Q000502:physiology*
chemical_list: D018994:Myosin Light Chains; D012333:RNA, Messenger; D012313:RNA; D003576:Electron Transport Complex IV; D005634:Fructose-Bisphosphate Aldolase