Assessment of the solid-state composition of an active salicylanilide compound by FT-Raman spectroscopy.
A biologically active salicylanilide compound currently appears in three known solid-state forms: polymorph I (Pol I), polymorph II (Pol II) and the amorphous form (Amorph). The obtained FT-Raman spectra revealed several regions of interest (ROIs) qualitatively distinguishing the different forms, allowing samples with an unknown polymorphic composition to be quantitatively analysed by FT-Raman spectroscopy. The Markov-transformed peak areas of the Raman-bands in the ROIs from the samples were determined and compared with the transformed peak areas obtained for the reference solid-state forms. A constrainted linear regression model estimated the contribution of each reference to the different samples. The applicability of this approach was demonstrated by analysing commercially available batches.
De Spiegeleer
B
B
Drug Quality & Registration (DruQuaR) Group, Department of Pharmaceutical Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, B-9000 Gent, Belgium. bart.despiegeleer@ugent.be
Baert
B
B
Diericx
N
N
Seghers
D
D
Verpoort
F
F
Van Vooren
L
L
Burvenich
C
C
Slegers
G
G
D008390:Markov Chains; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000737:chemistry*; D012016:Reference Values; D012458:Salicylanilides / Q000032:analysis*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*
D004364:Pharmaceutical Preparations; D012458:Salicylanilides; C034596:salicylanilide