title: [Hematoporphyrin derivative-mediated photodynamic therapy for human nasopharyngeal carcinoma: a comparative study with CNE2 and C666-1 cell lines in vitro].
abstract: OBJECTIVE
  To investigate biological effect of hematoporphyrin derivative (HpD) photodynamic therapy (PDT) on in vitro cultured nasopharyngeal carcinoma (NPC) cell lines CNE2 and C666-1.
  
  
  METHODS
  CNE2 and C666-1 cells cultured in vitro were incubated in a medium containing HpD at different concentrations (0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, and 4.0 microg/ml) for 4 h followed by exposure to different light doses (2, 5, 10, and 20 J/cm2) using a diode laser at 630 nm with power density of 20 mW/cm2. After 24 h of incubation with HpD-PDT, the survival rate of CNE2 and C666-1 cells were analyzed by MTT assay.
  
  
  RESULTS
  HpD-PDT produced effective killing of CNE2 and C666-1 cells cultured in vitro, and the killing effects were positively correlated with HpD concentration and the irradiation dose. Exposure of CNE2 and C666-1 cells to irradiation dose of 20 J/cm2 resulted in the IC50 of 0.7 and 1.2 microg/ml, respectively (P<0.01). With the same HpD concentration and irradiation dose, the survival rate of C666-1 cells, however, was significantly higher than that of CNE2 cells (P<0.05).
  
  
  CONCLUSIONS
  HpD-PDT may result in effective killing of CNE2 and C666-1 cells cultured in vitro, although C666-1 cells are less sensitive to HpD-PDT than CNE2 cells.
authors.affiliation: Oncotherapy Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. xiaomin-yang@163.com
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Yang
authors.forename: Rong-Cheng
authors.initials: RC
authors.lastname: Luo
authors.forename: Hong-Jing
authors.initials: HJ
authors.lastname: Ma
authors.forename: Li-Bo
authors.initials: LB
authors.lastname: Li
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Ding
authors.forename: Xiao
authors.initials: X
authors.lastname: Yan
authors.forename: Cheng-Wei
authors.initials: CW
authors.lastname: Lü
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Zhou
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D017324:Hematoporphyrin Derivative / Q000494:pharmacology*; D006414:Hematoporphyrin Photoradiation / Q000379:methods; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000473:pathology; D010778:Photochemotherapy / Q000379:methods; D017319:Photosensitizing Agents / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D017319:Photosensitizing Agents; D017324:Hematoporphyrin Derivative