Yeast proteome map (update 2006).
To improve the potential of two-dimensional gel electrophoresis for proteomic investigations in yeast we have undertaken the systematic identification of Saccharomyces cerevisiae proteins separated on 2-D gels. We report here the identification of 187 novel protein spots. They were identified by two methods, mass spectrometry and gene inactivation. These identifications extend the number of protein spots identified on our yeast 2-D proteome map to 602, i.e. nearly half the detectable spots of the proteome map. These spots correspond to 417 different proteins. The reference map and the list of identified proteins can be accessed on the Yeast Protein Map server (www.ibgc.u-bordeaux2.fr/YPM).
Perrot
Michel
M
Institut de Biochemie et Génétique Cellulaires, UMR CNRS 5095, Bordeaux, France.
Guieysse-Peugeot
Anne-Laure
AL
Massoni
Aurélie
A
Espagne
Christelle
C
Claverol
Stéphane
S
Silva
Raquel Monteiro
RM
Jenö
Paul
P
Santos
Manuel
M
Bonneu
Marc
M
Boucherie
Hélian
H
D015180:Electrophoresis, Gel, Two-Dimensional; D020868:Gene Silencing; D016681:Genome, Fungal; D013058:Mass Spectrometry; D010449:Peptide Mapping; D020543:Proteome; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*
D020543:Proteome; D029701:Saccharomyces cerevisiae Proteins