Enhancing the success of human islet isolation through optimization and characterization of pancreas dissociation enzyme.
A major obstacle to successful human islet isolation has been the variability of the enzymatic digestion phase. The aim of this study was to define optimal enzyme activity ranges normalized by the pancreas weight and to identify valid parameters for the optimal selection of successful lots of collagenase enzyme blends. Our results from 251 islet isolations showed that optimization of thermolysin dosage based on Caseinase unit/g pancreas contributed considerably to islet isolation outcome but that collagenase dosage measured by the manufacturer (Wünsch unit/g pancreas) was not a major determinant of islet isolation outcome. We also found that lot-to-lot inconsistency of enzyme performance was not explained by the activity values provided by the manufacturer, but rather by an in-house assay of class I collagenase (CI) and class II collagenase (CII); using a lot with a lower CII/CI resulted in a higher success rate. The odds of successful isolation was 8.67 times higher when a vial with CII/CI ratio <0.204 was used than when a vial with CII/CI >or=0.204 was used, suggesting that CII/CI ratio may be a strong predictor to distinguish potential lot success. This study provides a framework for improved enzymatic digestion in human islet isolation.
Kin
T
T
Clinical Islet Transplant Program, University of Alberta and Capital Health, Canada. tkin@ualberta.ca
Zhai
X
X
Murdoch
T B
TB
Salam
A
A
Shapiro
A M James
AM
Lakey
Jonathan R T
JR
D000293:Adolescent; D000328:Adult; D000368:Aged; D002469:Cell Separation / Q000379:methods*; D002648:Child; D017364:Collagenases / Q000378:metabolism / Q000494:pharmacology*; D003922:Diabetes Mellitus, Type 1 / Q000601:surgery; D004305:Dose-Response Relationship, Drug; D005260:Female; D006085:Graft Survival; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology*; D016381:Islets of Langerhans Transplantation / Q000379:methods; D008297:Male; D020784:Matrix Metalloproteinase 8 / Q000378:metabolism / Q000494:pharmacology*; D008875:Middle Aged; D009929:Organ Size; D010179:Pancreas / Q000166:cytology; D013820:Thermolysin / Q000494:pharmacology*
D017364:Collagenases; C417249:collagenase 1; D013820:Thermolysin; D020784:Matrix Metalloproteinase 8