Cholesterol biosensor based on N-(2-aminoethyl)-3-aminopropyl-trimethoxysilane self-assembled monolayer.
Cholesterol oxidase (ChOx) has been covalently immobilized onto two-dimensional self-assembled monolayer (SAM) of N-(2-aminoethyl)-3-aminopropyl-trimethoxysilane (AEAPTS) deposited on the indium-tin oxide (ITO) coated glass plates using N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide (EDC/NHS) chemistry. These ChO x/AEAPTS/ITO bioelectrodes are characterized using contact angle (CA) measurements, UV-visible spectroscopy, atomic force microscopy (AFM), electrochemical impedance technique, and Fourier transform infrared (FT-IR) technique. The covalently immobilized ChOx-modified AEAPTS bioelectrodes are used for the estimation of cholesterol in solution using UV-visible technique. These cholesterol sensing bioelectrodes show linearity as 50 to 500 mg/dl for cholesterol solution, detection limit as 25mg/dl, sensitivity as 4.499 x 10(-5) Abs (mg/dl)(-1), K(m) value as 58.137 mg/dl (1.5mM), apparent enzyme activity as 1.81 x 10(-3) U cm(-2), shelf life of approximately 10 weeks, and electrode reusability as 10 times.
Arya
Sunil K
SK
Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, K. S. Krishnan Marg, New Delhi 110012, India; Department of Chemistry, University of Delhi, Delhi 110007, India.
Prusty
Arun K
AK
Singh
S P
SP
Solanki
Pratima R
PR
Pandey
Manoj K
MK
Datta
Monika
M
Malhotra
Bansi D
BD
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002784:Cholesterol / Q000032:analysis* / Q000378:metabolism; D002789:Cholesterol Oxidase / Q000737:chemistry / Q000378:metabolism; D004563:Electrochemistry; D004566:Electrodes; D018625:Microscopy, Atomic Force; D008956:Models, Chemical; D015394:Molecular Structure; D015203:Reproducibility of Results; D012821:Silanes / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D012821:Silanes; D002784:Cholesterol; D002789:Cholesterol Oxidase; C071717:trimethoxysilane