title: High mobility group HMGI(Y) protein expression in head and neck squamous cell carcinoma.
abstract: CONCLUSIONS
  We conclude that increased expression level of the high mobility group I (HMGI(Y)) is closely associated with malignant transformation in head and neck squamous cell carcinomas (HNSCCs), and the measurement of HMGI(Y) levels in HNSCCs may be useful as a prognostic marker.
  
  
  OBJECTIVE
  To investigate whether HMGI overexpression is observed in HNSCCs, and its value as a prognostic marker in HNSCCs.
  
  
  METHODS
  HMGI(Y) expression was determined at the protein level by immunohistochemisty using a HMGI(Y)-specific antibody and RT-PCR in 10 surgically resected specimens of non-neoplastic tissue (normal palatal tissue) and 40 HNSCCs. We also evaluated the association of HMGI(Y) overexpression within clinicopathologic parameters, i.e. clinical stage, pathologic grade, status of cervical lymph node metastasis, recurrence rate.
  
  
  RESULTS
  Expression of HMGI(Y) by immunohistochemical staining was observed in 35 of 40 (87.5%) HNSCC samples, whereas normal mucosa and/or the mucosa adjacent to the tumor tissue showed negative or weakly positive staining (p<0.05). Semi-quantification of HMGI(Y) by RT-PCR was 2.98+/-2.24 in cancer and 0.47+/-0.25 in normal tissue (p<0.001). High expression of HMGI(Y) was observed in recurrent cases, compared with non-recurrent cases (p<0.05). However, no significant correlation was observed between the levels of HMGI(Y) expression and other clinical factors such as clinical stage, pathologic grade, and status of cervical lymph node metastasis.
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, College of Medicine, Hallym University, Seoul, South Korea. ys20805@chol.com
authors.forename: Young Soo
authors.initials: YS
authors.lastname: Rho
authors.forename: Young Chang
authors.initials: YC
authors.lastname: Lim
authors.forename: Il Seok
authors.initials: IS
authors.lastname: Park
authors.forename: Jin Hwan
authors.initials: JH
authors.lastname: Kim
authors.forename: Hwoe Young
authors.initials: HY
authors.lastname: Ahn
authors.forename: Seong Jin
authors.initials: SJ
authors.lastname: Cho
authors.forename: Hyung Sik
authors.initials: HS
authors.lastname: Shin
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000906:Antibodies / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D005260:Female; D025741:HMGA1a Protein / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009092:Mucous Membrane / Q000276:immunology / Q000378:metabolism; D009367:Neoplasm Staging; D011379:Prognosis; D012313:RNA / Q000235:genetics / Q000276:immunology / Q000378:metabolism
chemical_list: D000906:Antibodies; D017931:DNA Primers; D025741:HMGA1a Protein; D012313:RNA