The use of the zinc-fluorophore, Zinpyr-1, in the study of zinc homeostasis in Arabidopsis roots.
* The usefulness of the zinc (Zn)-fluorophore, Zinpyr-1, to examine the localization of Zn in the roots of Arabidopsis has been investigated. * In wild-type roots Zinpyr-1 fluorescence was predominantly in the xylem. The fluorescence signal was abolished by the application of the Zn-chelator, N,N,N',N-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN), and was increased by increasing exogenous Zn in the medium, indicating that fluorescence reflected relative Zn concentrations. * In the hma2, hma4 double mutant, which is deficient in root to shoot Zn translocation, Zinpyr-1 fluorescence was low in the xylem and high in the adjacent pericycle cells in which HMA2 and HMA4 are specifically expressed in a wild type. Zinpyr-1 fluorescence was also increased in the endodermis. * These results show that Zinpyr-1 can be used to examine the effects of mutations in Zn transporters on the localization of Zn in Arabidopsis roots and should be a useful addition to the tools available for studying Zn homeostasis in plants.
Sinclair
Scott A
SA
Department of Genetics, The University of Melbourne, Parkville, Australia 3010.
Sherson
Sarah M
SM
Department of Genetics, The University of Melbourne, Parkville, Australia 3010.
Jarvis
Renée
R
Department of Genetics, The University of Melbourne, Parkville, Australia 3010.
Camakaris
James
J
Department of Genetics, The University of Melbourne, Parkville, Australia 3010.
Cobbett
Christopher S
CS
Department of Genetics, The University of Melbourne, Parkville, Australia 3010.
D000251:Adenosine Triphosphatases / Q000235:genetics; D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D002614:Chelating Agents / Q000494:pharmacology; D005029:Ethylenediamines / Q000494:pharmacology; D005240:Feasibility Studies; D005452:Fluoresceins / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D049452:Green Fluorescent Proteins / Q000235:genetics; D006706:Homeostasis; D018613:Microscopy, Confocal / Q000379:methods; D018517:Plant Roots / Q000378:metabolism*; D030821:Plants, Genetically Modified; D052584:Xylem / Q000378:metabolism; D015032:Zinc / Q000378:metabolism*
D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D002614:Chelating Agents; D005029:Ethylenediamines; D005452:Fluoresceins; D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D000251:Adenosine Triphosphatases; C494967:HMA2 protein, Arabidopsis; C484509:HMA3 protein, Arabidopsis; C477864:HMA4 protein, Arabidopsis; D015032:Zinc; C044387:N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine; C501987:zinpyr-1