title: Proteomic analysis of Burkholderia cepacia MBA4 in the degradation of monochloroacetate.
abstract: Burkholderia cepacia MBA4 is a bacterium that degrades 2-haloacids by removing the halogen and subsequent metabolism of the product for energy. In this study, 2-DE, MS/MS, and N-terminal amino acid sequencing were used to investigate the protein expression profiles of MBA4 grown in a 2-haloacid (monochloroacetate, MCA) and in the corresponding metabolic product (glycolate). Glycolate was used as a control to eliminate the proteins induced by it. Five proteins were found to be up-regulated and five proteins were down-regulated in response to MCA. The differentially expressed proteins were examined, seven of them were identified by MS/MS and two of them were sequenced by Edman degradation. Our results definitely provide an insight for understanding the physiology of B. cepacia MBA4 in response to organohalide contaminated site.
authors.affiliation: Department of Botany, Molecular Microbiology Laboratory, The University of Hong Kong, Hong Kong. h0492057@hkusua.hku.hk
authors.forename: Sui-Yi
authors.initials: SY
authors.lastname: Kwok
authors.forename: Annie Fung-Ming
authors.initials: AF
authors.lastname: Siu
authors.forename: Sai-Ming
authors.initials: SM
authors.lastname: Ngai
authors.forename: Chi-Ming
authors.initials: CM
authors.lastname: Che
authors.forename: Jimmy S H
authors.initials: JS
authors.lastname: Tsang
mesh_terms: D000085:Acetates / Q000378:metabolism*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D016956:Burkholderia cepacia / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D015964:Gene Expression Regulation, Bacterial; D006016:Glycolates / Q000378:metabolism; D008969:Molecular Sequence Data; D040901:Proteomics; D053719:Tandem Mass Spectrometry
chemical_list: D000085:Acetates; D001426:Bacterial Proteins; D006016:Glycolates; C031149:glycolic acid; C006972:chloroacetic acid