[Experimental study of two histological quantitative methods of hepatic fibrosis].
To evaluate the value of two histological quantitative methods of hepatic fibrosis: semiquantative scoring system (SSS) and image analysis by computer. The prophylactic and therapeutic effect of Ganzhifu on hepatic fibrosis induced by CCl4 were studied on a total 73 of specimens from liver tissue of rats. All specimen were analyzed quantatively by two methods of SSS marks and image analysis respectively. Difference between groups was compared and hydroxyproline (Hyp) content of each liver tissue was examined. Correlation analysis was done between SSS marks, image analysis and Hyp content. Both prophylactic and therapeutic study showed the same information. Results of SSS marks, image analysis and Hyp content were coincidence. It suggest that both SSS marks and image analysis were interrelated well with Hyp content (P < 0.01). The result suggests that both SSS marks of hepatic fibrosis and image analysis by computer can be taken as reliable histological quantitative method of hepatic fibrosis.
Wang
Lichun
L
Key Laboratory of Biotherapy of Human Diseases, Ministry of Education, Department of Infectious Disease, West China Hospital, Sichuan University, Chengdu 610041, China.
Zhao
Liansan
L
Tang
Hong
H
Liu
Li
L
Li
Cong
C
Yao
Fan
F
Zhang
Hongying
H
D000818:Animals; D002251:Carbon Tetrachloride; D002252:Carbon Tetrachloride Poisoning; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use; D005260:Female; D006909:Hydroxyproline / Q000032:analysis*; D007091:Image Processing, Computer-Assisted; D008099:Liver / Q000737:chemistry / Q000473:pathology*; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology*; D008297:Male; D008517:Phytotherapy; D051381:Rats; D017208:Rats, Wistar
D004365:Drugs, Chinese Herbal; D002251:Carbon Tetrachloride; D006909:Hydroxyproline