Beta1 integrins control the formation of cell chains in the adult rostral migratory stream.
The subventricular zone (SVZ) of the lateral ventricle is the major site of neurogenesis in the adult brain. Neuroblasts that are born in the SVZ migrate as chains along the rostral migratory stream (RMS) to the olfactory bulb. Little is known about the mechanisms that control interactions between neuroblasts during their migration. Here we show that migrating neuroblasts express beta1 integrins and that the integrin ligand laminin is localized to cell chains. Using genetically modified mice and time-lapse video recordings of SVZ explants, we demonstrate that beta1 integrins and laminin promote the formation of cell chains. Laminin also induces the aggregation of purified neuroblasts. We conclude that the formation of cell chains in the RMS is controlled in part by beta1 integrins via binding to laminin. In addition, we provide evidence that beta1 class integrins are required for the maintenance of the glial tubes and that defects in the glial tubes lead to the ectopic migration of neuroblasts into the surrounding tissue.
Belvindrah
Richard
R
The Scripps Research Institute, Department of Cell Biology, Institute for Childhood and Neglected Disease, La Jolla, California 92037, USA.
Hankel
Sabine
S
Walker
John
J
Patton
Bruce L
BL
Müller
Ulrich
U
D000818:Animals; D002449:Cell Aggregation / Q000502:physiology; D002465:Cell Movement / Q000502:physiology*; D039641:Integrin beta Chains / Q000378:metabolism; D019012:Integrin beta1 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D007797:Laminin / Q000378:metabolism / Q000502:physiology; D020547:Lateral Ventricles / Q000166:cytology* / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout; D009457:Neuroglia / Q000502:physiology; D009474:Neurons / Q000473:pathology / Q000502:physiology*; D009830:Olfactory Bulb / Q000473:pathology; D009929:Organ Size
D039641:Integrin beta Chains; D019012:Integrin beta1; D007797:Laminin; C084344:integrin beta5