Phenotyping transgenic embryonic murine hearts using optical coherence tomography.
We used optical coherence tomography (OCT) to characterize the morphological phenotype of embryonic murine hearts discerning hexamethylene-bis-acetamide-inducible protein 1 (HEXIM1) mutants from their wild-type littermates. At E12.5 and E13.5 murine embryos were excised from the mother, the hearts were removed, and 3D OCT data sets were obtained from each heart in the litter. Next, we segmented the morphological borders to obtain cavity volumes and wall thicknesses. The mutant hearts exhibited increased ventricular chamber volume and decreased compact myocardium wall thickness when compared with their wild-type littermates. Also, the E13.5 HEXIM1 -/- embryo was distinguished by morphological asymmetry (underdeveloped left side).
Jenkins
Michael W
MW
Department of Biomedical Engineering, Case Western Reserve University, Ohio 44106, USA. mwj5@case.edu
Patel
Pankti
P
Deng
Huayun
H
Montano
Monica M
MM
Watanabe
Michiko
M
Rollins
Andrew M
AM
D000818:Animals; D006321:Heart / Q000033:anatomy & histology* / Q000196:embryology*; D051379:Mice / Q000235:genetics; D018345:Mice, Knockout; D008822:Mice, Transgenic / Q000033:anatomy & histology* / Q000196:embryology*; D010641:Phenotype; D016601:RNA-Binding Proteins / Q000235:genetics*; D041623:Tomography, Optical Coherence / Q000379:methods*; D014157:Transcription Factors
C476123:HEXIM1 protein, human; D016601:RNA-Binding Proteins; D014157:Transcription Factors