title: Frequent epigenetic silencing of protocadherin 10 by methylation in multiple haematologic malignancies.
abstract: Epigenetic silencing of tumour suppressor genes (TSG) inactivates TSG functions. Previously, we identified PCDH10 as a methylated TSG in carcinomas. Here, we detected its frequent silencing and methylation in lymphoma cell lines including 100% Burkitt, 100% diffuse large B cell, 86% Hodgkin, 100% nasal natural killer/T-cell lymphoma and 1/3 of leukaemia cell lines, and in primary tumours but not in normal peripheral blood mononuclear cells or lymph nodes. PCDH10 silencing could be reversed by demethylation with 5-aza-2'-deoxycytidine. Methylation was further detected in 14% of Hodgkin lymphoma sera. Thus, PCDH10 methylation is frequently involved in lymphomagenesis and could serve as a tumour-specific biomarker.
authors.affiliation: Cancer Epigenetics Laboratory, Department of Clinical Oncology, The Chinese University of Hong Kong, Hong Kong.
authors.forename: Jianming
authors.initials: J
authors.lastname: Ying
authors.forename: Zifen
authors.initials: Z
authors.lastname: Gao
authors.forename: Hongyu
authors.initials: H
authors.lastname: Li
authors.forename: Gopesh
authors.initials: G
authors.lastname: Srivastava
authors.forename: Paul G
authors.initials: PG
authors.lastname: Murray
authors.forename: Hwee Koon
authors.initials: HK
authors.lastname: Goh
authors.forename: Chai Yen
authors.initials: CY
authors.lastname: Lim
authors.forename: Yajun
authors.initials: Y
authors.lastname: Wang
authors.forename: Teresa
authors.initials: T
authors.lastname: Marafioti
authors.forename: David Y
authors.initials: DY
authors.lastname: Mason
authors.forename: Richard F
authors.initials: RF
authors.lastname: Ambinder
authors.forename: Anthony T C
authors.initials: AT
authors.lastname: Chan
authors.forename: Qian
authors.initials: Q
authors.lastname: Tao
mesh_terms: D014408:Biomarkers, Tumor; D002051:Burkitt Lymphoma / Q000235:genetics; D015820:Cadherins / Q000235:genetics*; D045744:Cell Line, Tumor; D018899:CpG Islands; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D019337:Hematologic Neoplasms / Q000235:genetics*; D006689:Hodgkin Disease / Q000235:genetics; D006801:Humans; D007938:Leukemia / Q000235:genetics; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics; D016399:Lymphoma, T-Cell / Q000235:genetics; D000091363:Protocadherins; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D015820:Cadherins; C438603:PCDH10 protein, human; D000091363:Protocadherins