[Studies on diterpenes in needles of Pinus sylvestris].
OBJECTIVE
To research the constituents in needles of Pinus sylvestris.


METHODS
Repeated column chromatography and preparation HPLC are used for compound isolation, and their structures were elucidated on the basis of spectral data analysis.


RESULTS
Six compounds, pinifolic acid (1), 15-oxo-8 (17) -labden-18-oic acid (2) , 15-acetoxy-labd-8 ( 17)-en-18-oic acid (3), dehydroabietic acid (4), 7alpha-hydroxydehydroabietic acid (5), 7beta-hydroxydehydroabietic acid (6) were isolated from the needles of P. sylvestris.


CONCLUSIONS
Compound 3-6 were isolated from the needles of P. sylvestris for the first time, and compound 3 is a new natural product. The petroleum ether and EtOAc extracts showed significant cytotoxic effects to Hela and A549. Compounds 2, 4-6 revealed a positive distinction compared to the control group.
Zhang
Rong
R
The School of Pharmacy, Tianjin Medical University, Tianjin 300070, China.
Duan
Hong-quan
HQ
Yao
Zhi
Z
Pan
Qin
Q
Zhang
Fu-geng
FG
D045784:Abietanes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D015394:Molecular Structure; D041605:Pinus sylvestris / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D013329:Structure-Activity Relationship
C528007:15-acetoxy-labd-8(17)-en-18-oic acid; C528300:7alpha-hydroxydehydroabietic acid; D045784:Abietanes; D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; C013913:dehydroabietic acid