A simple method of preparing diverse neoagaro-oligosaccharides with beta-agarase.
In order to prepare pure and well-defined oligosaccharides from agarose in a rapid and simple manner, an enzymatic degradation method was developed, which includes degradation with either recombinant beta-agarase (EC 3.2.1.81) AgaA or AgaB and gel permeation chromatography. Agarose was degraded with AgaA at the optimized conditions, yielding 47% and 45% of neoagarotetraose and neoagarohexaose, respectively. These neoagaro-oligosaccharides were conveniently separated by consecutive column chromatography on Bio-Gel P2 or P6 and were identified by FACE. The structure of these neoagaro-oligosaccharides was confirmed by MALDI-TOF MS and (13)C NMR spectroscopy.
Li
Jingbao
J
Marine Drug and Food Institute, Ocean University of China, 5 Yushan Road, Qingdao 266003, China.
Han
Feng
F
Lu
Xinzhi
X
Fu
Xiaoyan
X
Ma
Cuiping
C
Chu
Yan
Y
Yu
Wengong
W
D002236:Carbohydrate Conformation; D004926:Escherichia coli / Q000201:enzymology; D006026:Glycoside Hydrolases / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000138:chemical synthesis*; D011994:Recombinant Proteins / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D009844:Oligosaccharides; D011994:Recombinant Proteins; D006026:Glycoside Hydrolases; C019624:agarase