Differential impact of Lhx2 deficiency on expression of class I and class II odorant receptor genes in mouse.
Odorant receptor (OR) genes can be classified into two types: fish-like class I OR genes and mammalian-specific class II OR genes. We have previously shown that Lhx2, a LIM-homeodomain protein, binds to the homeodomain site in the promoter region of mouse M71, a class II OR, and that a knockout mutation in Lhx2 precludes expression of all tested class II OR genes including M71. Here, we report that most class I OR genes, which are expressed in a dorsal region of the olfactory epithelium, are still expressed in Lhx2-deficient embryos. There are two exceptions: two class I OR genes, which are normally expressed in a more ventral region, are no longer expressed in Lhx2 mutant mice. Lhx2 is transcribed in olfactory sensory neurons irrespective of expression of class I or class II OR genes. Thus, a deficiency of Lhx2 has a differential impact on class I and class II OR gene expression.
Hirota
Junji
J
The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA; Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan.
Omura
Masayo
M
Mombaerts
Peter
P
D000818:Animals; D004622:Embryo, Mammalian; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D060850:LIM-Homeodomain Proteins; D051379:Mice; D009831:Olfactory Mucosa / Q000196:embryology / Q000378:metabolism; D018034:Olfactory Receptor Neurons / Q000196:embryology* / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D018035:Receptors, Odorant / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000172:deficiency* / Q000235:genetics
D018398:Homeodomain Proteins; D060850:LIM-Homeodomain Proteins; C489784:Lhx2 protein, mouse; D012333:RNA, Messenger; D018035:Receptors, Odorant; D014157:Transcription Factors