Gadolinium chloride attenuates aortic occlusion-reperfusion-induced myocardial injury in rats.
OBJECTIVE
Aortic ischemia and reperfusion periods, which are often associated with infrarenal abdominal aortic cross-clamping and declamping, cause injury in distant organs including the heart. We recently reported that Kupffer cell blockage with gadolinium chloride (GdCl3) attenuates lung injury induced by aortic ischemia-reperfusion (IR). Therefore, we hypothesized that GdCl3 may attenuate myocardial injury induced by aortic IR.


METHODS
The study was carried out in June 2005, in the Laboratory of Experimental Studies of Suleyman Demirel University Medical School, Isparta, Turkey. We studied the effect of GdCl3 on myocardial injury induced by abdominal aortic occlusion-reperfusion in rats by measuring the tissue levels of superoxide dismutase, catalase, malondialdehyde and activity of myeloperoxidase in rat heart specimens. Wistar-Albino rats (8 per group) were randomized into 3 groups. The control group underwent midline laparotomy and dissection of the infrarenal abdominal aorta without occlusion; the aortic IR group underwent laparotomy and clamping of the infrarenal abdominal aorta for 30 minutes followed by 60 minutes of reperfusion; and the GdCl3 + aortic IR group was pretreated with intravenous GdCl3 10 mg/kg 24 hours before the aortic IR.


RESULTS
Aortic IR significantly increased whereas pretreatment with GdCl3 significantly decreased oxygen free radical production, lipid peroxidation and neutrophil activation in the heart tissues of the rats.


CONCLUSIONS
Our results indicate that Kupffer cell blockage with GdCl3 attenuates the myocardial injury induced by aortic IR. We think that the novel findings of the present study may be a basis for further studies investigating the role of GdCl3 pretreatment in reducing myocardial morbidity and mortality caused by aortic IR during aortic surgery.
Kiris
Ilker
I
Department of Cardiovascular Surgery, Suleyman Demirel University Medical School, Isparta, Turkey. kirisilker@yahoo.com
Okutan
Huseyin
H
Savas
Cagri
C
Yonden
Zafer
Z
Delibas
Namik
N
D000818:Animals; D001157:Arterial Occlusive Diseases / Q000097:blood / Q000517:prevention & control; D015415:Biomarkers; D002316:Cardiotonic Agents / Q000494:pharmacology; D004195:Disease Models, Animal; D005260:Female; D005682:Gadolinium / Q000494:pharmacology*; D007728:Kupffer Cells / Q000166:cytology / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000032:analysis / Q000378:metabolism*; D017202:Myocardial Ischemia / Q000097:blood / Q000517:prevention & control*; D009195:Peroxidase / Q000032:analysis / Q000378:metabolism*; D011336:Probability; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D015427:Reperfusion Injury / Q000097:blood / Q000517:prevention & control; D012680:Sensitivity and Specificity; D013482:Superoxide Dismutase / Q000032:analysis / Q000378:metabolism*
D015415:Biomarkers; D002316:Cardiotonic Agents; D008315:Malondialdehyde; D005682:Gadolinium; D009195:Peroxidase; D013482:Superoxide Dismutase; C038958:gadolinium chloride