Hybrid phosphorescence and fluorescence native spectroscopy for breast cancer detection.
Fluorescence and phosphorescence measurements are performed on normal and malignant ex vivo human breast tissues using UV LED and xenon lamp excitation. Tryptophan (trp) phosphorescence intensity is higher in both normal glandular and adipose tissue when compared to malignant tissue. An algorithm based on the ratio of trp fluorescence intensity at 345 nm to phosphorescence intensity at 500 nm is successfully used to separate normal from malignant tissue types. Normal specimens consistently exhibited a low I(345)I(500) ratio (<10), while for malignant specimens, the I(345)I(500) ratio is consistently high (>15). The ratio analysis correlates well with histopathology. Intensity ratio maps with a spatial resolution of 0.5 mm are generated in which local regions of malignancy could be identified.
Alimova
Alexandra
A
The City College of New York, Department of Physics, Institute for Ultrafast Spectroscopy and Lasers, New York, New York 10031, USA.
Katz
A
A
Sriramoju
Vidyasagar
V
Budansky
Yuri
Y
Bykov
Alexei A
AA
Zeylikovich
Roman
R
Alfano
R R
RR
D014408:Biomarkers, Tumor / Q000032:analysis*; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D003936:Diagnosis, Computer-Assisted / Q000379:methods*; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014364:Tryptophan / Q000032:analysis*
D014408:Biomarkers, Tumor; D014364:Tryptophan