title: A silicone-based controlled-release device for accelerated proteolytic debridement of wounds.
abstract: A new device for rapid enzymatic debridement of cutaneous wounds has been developed using a controlled-release, silicone-based, dried emulsion. A dehydrated serine protease of the subtilisin family, previously untested for wound debridement, was incorporated into the emulsion. This device exhibited excellent storage stability. Moisture from the wound triggered an even, reproducible, and complete release of the enzyme within the first 8 hours. The device maintains a moist wound environment that allows the enzyme to achieve nearly complete digestion of the hardened eschar of full-thickness burns in a porcine model after an exposure period of 24 hours. Debridement was faster than in untreated wounds or wounds treated with a currently available enzyme ointment. Following rapid enzymatic debridement, healing appeared to progress normally, with no histological evidence of damage to adjacent healthy tissue.
authors.affiliation: Danisco, Palo Alto, California, USA. rick.bott@danisco.com
authors.forename: Richard
authors.initials: R
authors.lastname: Bott
authors.forename: James
authors.initials: J
authors.lastname: Crissman
authors.forename: Csilla
authors.initials: C
authors.lastname: Kollar
authors.forename: Mae
authors.initials: M
authors.lastname: Saldajeno
authors.forename: Grant
authors.initials: G
authors.lastname: Ganshaw
authors.forename: Xavier
authors.initials: X
authors.lastname: Thomas
authors.forename: Thomas H
authors.initials: TH
authors.lastname: Lane
authors.forename: Paal
authors.initials: P
authors.lastname: Klykken
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Davidson
authors.forename: Lillian B
authors.initials: LB
authors.lastname: Nanney
mesh_terms: D000818:Animals; D002056:Burns / Q000601:surgery*; D003646:Debridement / Q000295:instrumentation* / Q000379:methods; D003879:Dermatologic Agents / Q000008:administration & dosage*; D004195:Disease Models, Animal; D016503:Drug Delivery Systems; D004655:Emulsions; D009779:Occlusive Dressings; D009824:Ointments; D010206:Papain / Q000008:administration & dosage; D010865:Pilot Projects; D012828:Silicones; D020860:Subtilisin / Q000008:administration & dosage*; D013552:Swine
chemical_list: D003879:Dermatologic Agents; D004655:Emulsions; D009824:Ointments; D012828:Silicones; D020860:Subtilisin; D010206:Papain