title: Alginate as an antiglycating agent for human serum albumin.
abstract: Hyperglycemia and the accumulation of advanced glycation endproducts (AGEs) in tissues and serum have important roles in diabetic complications. Therefore, the identification of anti-glycation compounds is attracting considerable interest. In this study, the interaction of human serum albumin (HSA) with fructose, in the absence and presence of alginate, was studied by circular dichroism, absorbance and fluorescence techniques. The characterization study of AGEs was performed using autofluorescence, fibrillar formation, the increase in absorbance and the quantification of free lysine side chains. The results indicate that alginate inhibits the fructation of HSA as observed by a reduction in the formation of fluorescent AGEs and fibrils. Furthermore, alginate reduces the amount of modified lysine side chains, signified by the lack of increase in absorbance, and increases the helicity of this protein.
authors.affiliation: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
authors.forename: Naghmeh
authors.initials: N
authors.lastname: Sattarahmady
authors.forename: Fariba
authors.initials: F
authors.lastname: Khodagholi
authors.forename: Ali A
authors.initials: AA
authors.lastname: Moosavi-Movahedi
authors.forename: Hossein
authors.initials: H
authors.lastname: Heli
authors.forename: Gholam H
authors.initials: GH
authors.lastname: Hakimelahi
mesh_terms: D000464:Alginates / Q000737:chemistry*; D005632:Fructose / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D017127:Glycation End Products, Advanced / Q000737:chemistry* / Q000378:metabolism; D006031:Glycosylation; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D006943:Hyperglycemia / Q000378:metabolism; D008239:Lysine / Q000737:chemistry / Q000378:metabolism; D017433:Protein Structure, Secondary; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism; D013057:Spectrum Analysis
chemical_list: D000464:Alginates; D017127:Glycation End Products, Advanced; D006603:Hexuronic Acids; D012709:Serum Albumin; D005632:Fructose; D020723:Glucuronic Acid; D008239:Lysine