title: 3D histological reconstruction of fiber tracts and direct comparison with diffusion tensor MRI tractography.
abstract: A classical neural tract tracer, WGA-HRP, was injected at multiple sites within the brain of a macaque monkey. Histological sections of the labeled fiber tracts were reconstructed in 3D, and the fibers were segmented and registered with the anatomical post-mortem MRI from the same animal. Fiber tracing along the same pathways was performed on the DTI data using a classical diffusion tracing technique. The fibers derived from the DTI were compared with those segmented from the histology in order to evaluate the performance of DTI fiber tracing. While there was generally good agreement between the two methods, our results reveal certain limitations of DTI tractography, particularly at regions of fiber tract crossing or bifurcation.
authors.affiliation: Computational Radiology Laboratory, Children's Hospital, Brigham and Women's Hospital, Harvard Medical School, Boston, USA.
authors.forename: Julien
authors.initials: J
authors.lastname: Dauguet
authors.forename: Sharon
authors.initials: S
authors.lastname: Peled
authors.forename: Vladimir
authors.initials: V
authors.lastname: Berezovskii
authors.forename: Thierry
authors.initials: T
authors.lastname: Delzescaux
authors.forename: Simon K
authors.initials: SK
authors.lastname: Warfield
authors.forename: Richard
authors.initials: R
authors.lastname: Born
authors.forename: Carl-Fredrik
authors.initials: CF
authors.lastname: Westin
mesh_terms: D000465:Algorithms; D000818:Animals; D001185:Artificial Intelligence; D001921:Brain / Q000166:cytology*; D038524:Diffusion Magnetic Resonance Imaging / Q000379:methods*; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008251:Macaca; D009413:Nerve Fibers, Myelinated / Q000648:ultrastructure*; D009434:Neural Pathways / Q000166:cytology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity