Annexin 1: differential expression in tumor and mast cells in human larynx cancer.
Annexin 1 protein (ANXA1) expression was evaluated in tumor and mast cells in human larynx cancer and control epithelium. The effect of the exogenous ANXA1 (peptide Ac 2-26) was also examined during the cellular growth of the Hep-2 human larynx epidermoid carcinoma cell line. This peptide inhibited the proliferation of the Hep-2 cells within 144 hr. In surgical tissue specimens from 20 patients with larynx cancer, ultrastructural immunocytochemistry analysis showed in vivo down-regulation of ANXA1 expression in the tumor and increased in mast cells and Hep-2 cells treated with peptide Ac2-26. Combined in vivo and in vitro analysis demonstrated that ANXA1 plays a regulatory role in laryngeal cancer cell growth. We believe that a better understanding of the regulatory mechanisms of ANXA1 in tumor and mast cells may lead to future biological targets for the therapeutic intervention of human larynx cancer.
Silistino-Souza
Rosana
R
Department of Biology, Instituto de Biociências, Letras e Ciências Exatas (IBILCE), São Paulo State University (UNESP), São José do Rio Preto, São Paulo, Brazil.
Rodrigues-Lisoni
Flávia C
FC
Cury
Patricia M
PM
Maniglia
José V
JV
Raposo
Luis S
LS
Tajara
Eloiza H
EH
Christian
Helen C
HC
Oliani
Sonia M
SM
D000368:Aged; D000369:Aged, 80 and over; D017305:Annexin A1 / Q000032:analysis / Q000235:genetics* / Q000494:pharmacology; D045744:Cell Line, Tumor; D004847:Epithelial Cells / Q000378:metabolism / Q000648:ultrastructure; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D007830:Larynx / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D008407:Mast Cells / Q000378:metabolism*; D016253:Microscopy, Immunoelectron; D008875:Middle Aged; D010455:Peptides / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D053802:Tryptases / Q000235:genetics / Q000378:metabolism
D017305:Annexin A1; D010455:Peptides; D012333:RNA, Messenger; C096153:annexin A1 peptide (2-26); D053802:Tryptases