title: Antibacterial effect of autologous platelet gel enriched with growth factors and other active substances: an in vitro study.
abstract: Platelet-rich plasma is a new inductive therapy which is being increasingly used for the treatment of the complications of bone healing, such as infection and nonunion. The activator for platelet-rich plasma is a mixture of thrombin and calcium chloride which produces a platelet-rich gel. We analysed the antibacterial effect of platelet-rich gel in vitro by using the platelet-rich plasma samples of 20 volunteers. In vitro laboratory susceptibility to platelet-rich gel was determined by the Kirby-Bauer disc-diffusion method. Baseline antimicrobial activity was assessed by measuring the zones of inhibition on agar plates coated with selected bacterial strains. Zones of inhibition produced by platelet-rich gel ranged between 6 mm and 24 mm (mean 9.83 mm) in diameter. Platelet-rich gel inhibited the growth of Staphylococcus aureus and was also active against Escherichia coli. There was no activity against Klebsiella pneumoniae, Enterococcus faecalis, and Pseudomonas aeruginosa. Moreover, platelet-rich gel seemed to induce the in vitro growth of Ps. aeruginosa, suggesting that it may cause an exacerbation of infections with this organism. We believe that a combination of the inductive and antimicrobial properties of platelet-rich gel can improve the treatment of infected delayed healing and nonunion.
authors.affiliation: Department and Clinic of Orthopaedics, Medical University of Silesia, Sosnowiec, Poland. tomekbiel@o2.pl
authors.forename: T M
authors.initials: TM
authors.lastname: Bielecki
authors.forename: T S
authors.initials: TS
authors.lastname: Gazdzik
authors.forename: J
authors.initials: J
authors.lastname: Arendt
authors.forename: T
authors.initials: T
authors.lastname: Szczepanski
authors.forename: W
authors.initials: W
authors.lastname: Król
authors.forename: T
authors.initials: T
authors.lastname: Wielkoszynski
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D001792:Blood Platelets / Q000502:physiology*; D052978:Disk Diffusion Antimicrobial Tests / Q000379:methods; D013293:Enterococcus faecalis / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D005782:Gels; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D007711:Klebsiella pneumoniae / Q000187:drug effects; D007958:Leukocyte Count; D010976:Platelet Count; D053657:Platelet-Rich Plasma; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D013917:Thrombin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D005782:Gels; D036341:Intercellular Signaling Peptides and Proteins; D013917:Thrombin