Intranasal immunization with chlamydial protease-like activity factor and CpG deoxynucleotides enhances protective immunity against genital Chlamydia muridarum infection.
We have reported recently that intranasal (i.n.) vaccination with chlamydial protease-like activity factor (CPAF) and interleukin-12 (IL-12) enhances protective immunity against genital chlamydial challenge. In this study, we show that i.n. or intraperitoneal (i.p.) vaccination with CPAF plus CpG deoxynucleotides (CpG), an alternative T helper 1 (Th1) adjuvant, induced robust CPAF-specific IFN-gamma responses and elevated levels of serum antibody and vaginal IgA production. CPAF+CpG vaccinated animals displayed accelerated genital chlamydial clearance, and minimal hydrosalpinx and inflammatory cellular infiltration compared to mock-immunized (PBS) challenged animals. Together, CpG dexoynucleotides are an efficacious alternative Th1 adjuvant with CPAF to induce protective anti-chlamydial immunity.
Cong
Yu
Y
South Texas Center for Emerging Infectious Diseases, Department of Biology, University of Texas at San Antonio, San Antonio, TX 78249, United States.
Jupelli
Madhulika
M
Guentzel
M Neal
MN
Zhong
Guangming
G
Murthy
Ashlesh K
AK
Arulanandam
Bernard P
BP
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000281:Administration, Intranasal; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology; D002690:Chlamydia Infections / Q000276:immunology / Q000382:microbiology; D023502:Chlamydia muridarum / Q000276:immunology*; D018899:CpG Islands / Q000276:immunology; D010450:Endopeptidases / Q000008:administration & dosage*; D005260:Female; D005831:Genital Diseases, Female / Q000276:immunology* / Q000382:microbiology; D006801:Humans; D007114:Immunization; D007071:Immunoglobulin A, Secretory / Q000378:metabolism; D007371:Interferon-gamma / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage*; D014621:Vagina / Q000276:immunology / Q000382:microbiology
D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; C408982:CPG-oligonucleotide; D007071:Immunoglobulin A, Secretory; D009838:Oligodeoxyribonucleotides; D007371:Interferon-gamma; D010450:Endopeptidases; C425923:CPA factor