title: Involvement of UL24 in herpes-simplex-virus-1-induced dispersal of nucleolin.
abstract: UL24 of herpes simplex virus 1 is important for efficient viral replication, but its function is unknown. We generated a recombinant virus, vHA-UL24, encoding UL24 with an N-terminal hemagglutinin tag. By indirect immunofluorescence at 9 h post-infection (hpi), we detected HA-UL24 in nuclear foci and in cytoplasmic speckles. HA-UL24 partially co-localized with nucleolin, but not with ICP8 or coilin, markers for nucleoli, viral replication compartments, and Cajal bodies respectively. HA-UL24 staining was often juxtaposed to that of another nucleolar protein, fibrillarin. Analysis of HSV-1-induced nucleolar modifications revealed that by 18 hpi, nucleolin staining had dispersed, and fibrillarin staining went from clusters of small spots to a few separate but prominent spots. Fibrillarin redistribution appeared to be independent of UL24. In contrast, cells infected with a UL24-deficient virus retained foci of nucleolin staining. Our results demonstrate involvement of UL24 in dispersal of nucleolin during infection.
authors.affiliation: INRS-Institut Armand-Frappier, Université du Québec, 531 boul. des Prairies, Laval, Québec, Canada H7V 1B7. maria.lymberopoulos@iaf.inrs.ca <maria.lymberopoulos@iaf.inrs.ca>
authors.forename: Maria H
authors.initials: MH
authors.lastname: Lymberopoulos
authors.forename: Angela
authors.initials: A
authors.lastname: Pearson
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002522:Chlorocebus aethiops; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D006561:Herpes Simplex / Q000821:virology; D018259:Herpesvirus 1, Human / Q000378:metabolism*; D009697:Nucleolus Organizer Region / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D014709:Vero Cells; D014764:Viral Proteins / Q000502:physiology*; D000097796:Nucleolin
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D014764:Viral Proteins; D000097796:Nucleolin; C086267:UL24 protein, Human herpesvirus 1; C047458:fibrillarin