title: A comprehensive proteome map of the lipid-requiring nosocomial pathogen Corynebacterium jeikeium K411.
abstract: Corynebacterium jeikeium is a lipid-requiring pathogen that is considered as part of the normal microflora of the human skin and associated with severe nosocomial infections. Systematic reference maps of the cytoplasmic, cell surface-associated, and extracellular proteome fractions of the clinical isolate C. jeikeium K411 were examined by 2-DE coupled with MALDI-TOF MS. A sum total of 555 protein spots were identified by PMF, corresponding to 358 different proteins that were classified into functional categories and integrated into metabolic pathways. The majority of the proteins were linked to housekeeping functions in energy production and translation and to physiological processes in amino acid, carbohydrate, nucleotide, and lipid metabolism. A complete enzymatic machinery necessary to utilize exogenous fatty acids by beta-oxidation was detected in the cytoplasmic proteome fraction. In addition, several predicted virulence factors of C. jeikeium K411 were identified in the cell surface-associated and extracellular subproteome, including the cell surface proteins SurA and SurB, the surface-anchored pilus subunits SapA and SapB, the surface-anchored collagen adhesin CbpA, the cholesterol esterase Che, and the acid phosphatase AcpA.
authors.affiliation: Lehrstuhl für Genetik, Fakultät für Biologie, Universität Bielefeld, Bielefeld, Germany.
authors.forename: Nicole
authors.initials: N
authors.lastname: Hansmeier
authors.forename: Tzu-Chiao
authors.initials: TC
authors.lastname: Chao
authors.forename: Svenja
authors.initials: S
authors.lastname: Daschkey
authors.forename: Mathias
authors.initials: M
authors.lastname: Müsken
authors.forename: Jörn
authors.initials: J
authors.lastname: Kalinowski
authors.forename: Alfred
authors.initials: A
authors.lastname: Pühler
authors.forename: Andreas
authors.initials: A
authors.lastname: Tauch
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D003352:Corynebacterium / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry; D053858:Metabolic Networks and Pathways; D008969:Molecular Sequence Data; D020543:Proteome
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D020543:Proteome