title: Lateral diffusion of TGF-beta type I receptor studied by single-molecule imaging.
abstract: In this report, we investigated the lateral diffusion of transforming growth factor beta (TGF-beta) type I receptor (TbetaRI) in living cells by imaging and tracking individual green fluorescent protein tagged TbetaRI on the cell membrane. We found that when co-expressed with TGF-beta type II receptor (TbetaRII), the mobility of TbetaRI decreased significantly after TGF-beta1 stimulation. However, in the cells that had been depleted of cholesterol with Nystatin or methyl-beta-cyclodextrin, the diffusion rate of TbetaRI was not changed by TGF-beta1 treatment. Our observations suggest that membrane lipid-rafts provide an environment that facilitates the association of TbetaRI and TbetaRII for cell signaling.
authors.affiliation: Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China.
authors.forename: Xinyong
authors.initials: X
authors.lastname: Ma
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Yaxin
authors.initials: Y
authors.lastname: Jiang
authors.forename: Zeyu
authors.initials: Z
authors.lastname: Xiao
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Fang
authors.forename: Ye-Guang
authors.initials: YG
authors.lastname: Chen
mesh_terms: D030201:Activin Receptors, Type I / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D007476:Ionophores / Q000494:pharmacology; D021962:Membrane Microdomains / Q000187:drug effects / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods*; D009761:Nystatin / Q000494:pharmacology; D017346:Protein Serine-Threonine Kinases; D021381:Protein Transport / Q000187:drug effects; D000077293:Receptor, Transforming Growth Factor-beta Type I; D000077294:Receptor, Transforming Growth Factor-beta Type II; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology
chemical_list: D007476:Ionophores; D018125:Receptors, Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D009761:Nystatin; D049452:Green Fluorescent Proteins; D017346:Protein Serine-Threonine Kinases; D030201:Activin Receptors, Type I; D000077293:Receptor, Transforming Growth Factor-beta Type I; D000077294:Receptor, Transforming Growth Factor-beta Type II