title: c-Abl tyrosine kinase activates p21 transcription via interaction with p53.
abstract: c-Abl non-receptor tyrosine kinase has been implicated in many cellular processes including cell differentiation, stress response and regulating gene transcription. The mechanism by which c-Abl is involved in the regulation of gene transcription remains to be elucidated. In this study, we investigated the functions of c-Abl in the activation of p21 promoter. Our results showed that overexpression of c-Abl tyrosine kinase activated p21 promoter and endogenous p21 transcription in U2OS cells. We found that p53 is involved in the activation of p21 promoter by c-Abl, and integrative structure of p53 is required for regulating p21 transcription. In addition, the chromatin immunoprecipitation study demonstrated that c-Abl and p53 can be recruited to the region containing p53 binding site of p21 promoter, and c-Abl increases the DNA binding activity of p53 to the p21 promoter. Furthermore, not only the activation of p21 promoter but also the recruitment to p21 promoter by c-Abl is dependent on the interaction between c-Abl and p53 protein.
authors.affiliation: Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China.
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Jing
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Wen
authors.initials: W
authors.lastname: Tang
authors.forename: Tianyang
authors.initials: T
authors.lastname: Qi
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Gu
authors.forename: Shui
authors.initials: S
authors.lastname: Hao
authors.forename: Xianlu
authors.initials: X
authors.lastname: Zeng
mesh_terms: D045744:Cell Line, Tumor; D047369:Chromatin Immunoprecipitation; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D011401:Promoter Regions, Genetic / Q000502:physiology; D016315:Proto-Oncogene Proteins c-abl / Q000502:physiology*; D014158:Transcription, Genetic / Q000502:physiology*; D016159:Tumor Suppressor Protein p53 / Q000502:physiology*
chemical_list: C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016159:Tumor Suppressor Protein p53; D016315:Proto-Oncogene Proteins c-abl