title: Concurrent high expression of human telomerase reverse transcriptase and human nonmetastatic clone 23 in high-grade squamous intraepithelial neoplasia and squamous cell carcinoma of uterine cervix.
abstract: Human telomerase reverse transcriptase (hTERT) and human nonmetastatic clone 23 (nm23-H1) may be separately involved in tumor progression of uterine cervix. We therefore investigate the correlations of hTERT and nm23-H1 in cervical carcinogenesis and further check their application. One hundred and twenty-eight cervical tissues, including 48 squamous cell carcinoma (SCC), 36 high-grade cervical intraepithelial neoplasia (CIN) (CIN 2 and CIN 3), 20 low-grade CIN 1, and 24 normal cases, were collected for immunohistochemical expression of hTERT and nm23-H1. Spearman rank correlation analysis was applied to assess their correlation in these samples. The Fisher exact or Chi-square test was used to evaluate the expression of hTERT or nm23-H1 among each subgroup. The sensitivity, specificity, positive and negative predictive values (PPV and NPV), and accuracy of hTERT and/or nm23-H1 were calculated for the prediction of high-grade CIN and SCC. We found normal cervix and CIN 1 samples had concurrent low expression of hTERT and nm23-H1, whereas high-grade CIN and SCC samples had concurrent high immunoreactivities. The hTERT alone and hTERT or nm23-H1 in combination had better sensitivity, NPV, and accuracy. The nm23-H1 alone as well as hTERT and nm23-H1 in combination had better specificity and PPV. Our results reveal a significantly positive relationship between expression of hTERT and nm23-H1 in normal and neoplastic tissues of uterine cervix. We suggest high expression of hTERT alone and hTERT or nm23-H1 in combination can be offered additional molecular information correlated with high-grade CIN and SCC.
authors.affiliation: Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.
authors.forename: C-G
authors.initials: CG
authors.lastname: Hsu
authors.forename: P-H
authors.initials: PH
authors.lastname: Wang
authors.forename: J-L
authors.initials: JL
authors.lastname: Ko
authors.forename: G-D
authors.initials: GD
authors.lastname: Chen
authors.forename: H
authors.initials: H
authors.lastname: Chang
authors.forename: S-F
authors.initials: SF
authors.lastname: Yang
authors.forename: S-C
authors.initials: SC
authors.lastname: Chen
authors.forename: L-Y
authors.initials: LY
authors.lastname: Lin
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D018450:Disease Progression; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D054778:NM23 Nucleoside Diphosphate Kinases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D012680:Sensitivity and Specificity; D019098:Telomerase / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002578:Uterine Cervical Dysplasia / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: D054778:NM23 Nucleoside Diphosphate Kinases; C517938:NME1 protein, human; C509186:TERT protein, human; D019098:Telomerase