The trithorax-group gene in Drosophila little imaginal discs encodes a trimethylated histone H3 Lys4 demethylase.
Histone H3 Lys4 (H3K4) is methylated by yeast Set1-COMPASS and its mammalian homolog, the MLL complex. Human JARID1d can demethylate trimethyl-H3K4 (H3K4me3). We identified Drosophila melanogaster little imaginal discs (Lid) as the JARID1d homolog. We report that Lid knockdown using RNA interference results in a specific genome-wide increase in H3K4me3 levels without affecting other patterns of H3 methylation, and results in an altered distribution of the chromo-helicase protein Chd1.
Eissenberg
Joel C
JC
Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, 1402 South Grand Blvd., St. Louis, Missouri 63104, USA.
Lee
Min Gyu
MG
Schneider
Jessica
J
Ilvarsonn
Anne
A
Shiekhattar
Ramin
R
Shilatifard
Ali
A
D000818:Animals; D002875:Chromosomes / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D029721:Drosophila Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000201:enzymology*; D056466:Histone Demethylases; D011495:Histone-Lysine N-Methyltransferase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D007814:Larva / Q000166:cytology; D008239:Lysine / Q000378:metabolism*; D008745:Methylation; D011485:Protein Binding; D034622:RNA Interference; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
C100593:CHD1 protein, Drosophila; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D006657:Histones; D014157:Transcription Factors; C063460:Trl protein, Drosophila; D056466:Histone Demethylases; C518655:Lid protein, Drosophila; D011495:Histone-Lysine N-Methyltransferase; D008239:Lysine