title: The effect of growth medium on the antioxidant defense of Saccharomyces cerevisiae.
abstract: We compared the oxidation of dihydrorhodamine 123, glutathione contents and activities of superoxide dismutase (SOD) and catalase for three wild-type strains of Saccharomyces cerevisiae grown on media with different carbon sources. The rate of oxidation of dihydrorhodamine 123 was much higher in respiring cells grown on ethanol or glycerol media than in fermenting cells grown on glucose medium. The total SOD activity was highest on glycerol medium and lowest on ethanol medium, while the catalase activity was highest on glycerol medium. The sequence of glutathione content values was: glucose > ethanol > glycerol.
authors.affiliation: Department of Molecular Biophysics, University of Łódź, Banacha 12/16, 90-237, Łódź, Poland. ematrix@biol.uni.lodz.pl
authors.forename: Ewa
authors.initials: E
authors.lastname: Macierzyńska
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Grzelak
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Bartosz
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D003470:Culture Media / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D012235:Rhodamines / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D003470:Culture Media; D012235:Rhodamines; C058319:dihydrorhodamine 123; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione