Notch signaling inhibits growth of the human lung adenocarcinoma cell line A549.
In lung cancers the Notch signaling may function as an oncogene or a tumor suppressor depending on the tumor cell types. In this study we examined the expression of Notch receptors in the human lung adenocarcinoma cell lines A549 and SPC-A-1. We over-expressed the active form of Notch1 (NIC) in A549 cells by constitutive transfection to evaluate the effects of the Notch signaling on lung adenocarcinoma cells. Our results showed that over-expression of NIC in A549 cells inhibited the growth of A549 cells through induction of cell cycle arrest. Moreover, over-expression of NIC inhibited the colony-forming activity of A549 cells when cultured in methylcellulose medium, and their ability to form tumors in nude mice. These data suggest that the Notch signaling may function as a tumor inhibitor in human lung adenocarcinoma cells.
Zheng
Qijun
Q
State Key Laboratory of Cancer Biology, Department of Medical Genetics and Developmental Biology, Xijing Hospital, Fourth Military Medical University, Xian 710032, P.R. China.
Qin
Hongyan
H
Zhang
Hongwei
H
Li
Junchang
J
Hou
Lihong
L
Wang
Hongbin
H
Zhang
Xueping
X
Zhang
Suzhen
S
Feng
Lei
L
Liang
Yingmin
Y
Han
Hua
H
Yi
Dinghua
D
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
D051880:Receptors, Notch; D025521:Tumor Suppressor Proteins