Efficient gene transduction of neurons by lentivirus with enhanced neuron-specific promoters.
In the field of basic and clinical neurosciences, it is important to develop a method for easy delivery and persistent expression of transgene in central neurons. We firstly generated lentiviral vectors with five kinds of neuron-specific promoters, such as synapsin I (SYN), calcium/calmodulin-dependent protein kinase II, tubulin alpha I, neuron-specific enolase and platelet-derived growth factor beta chain promoters and then novel hybrid promoters by fusing cytomegalovirus enhancer (E) to those neuron-specific promoters. Neuron-specific expression of green fluorescent protein (GFP) with those promoters was examined in vivo by injecting the lentiviral vectors into the rat neostriatum, thalamus and neocortex. Among all the promoters, SYN promoter displayed the highest specificity for neuronal expression in all the regions examined (more than 96%). Although GFP production by the hybrid promoters was about 2-4 times larger than the non-enhanced promoters, the neuronal specificity was significantly decreased in most cases. However, the neuronal specificity of E/SYN hybrid promoter exhibited the least decrease only in the thalamus. Furthermore, the transcriptional activity and neuronal specificity of E/SYN promoter were sustained for up to 8 weeks. Thus, lentivirus with E/SYN promoter is the best vector for strong persistent expression in neurons.
Hioki
H
H
Department of Morphological Brain Science, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Kameda
H
H
Nakamura
H
H
Okunomiya
T
T
Ohira
K
K
Nakamura
K
K
Kuroda
M
M
Furuta
T
T
Kaneko
T
T
D000818:Animals; D001921:Brain / Q000378:metabolism; D003587:Cytomegalovirus / Q000235:genetics; D004742:Enhancer Elements, Genetic; D015870:Gene Expression; D005818:Genetic Engineering; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000008:administration & dosage* / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D015497:HIV-1 / Q000235:genetics*; D017919:Hepatitis B Virus, Woodchuck / Q000235:genetics; D007267:Injections; D008297:Male; D018613:Microscopy, Confocal; D023421:Models, Animal; D009474:Neurons / Q000378:metabolism* / Q000821:virology; D010300:Parkinson Disease / Q000628:therapy; D011401:Promoter Regions, Genetic; D051381:Rats; D017208:Rats, Wistar; D016704:Synapsins / Q000235:genetics; D014161:Transduction, Genetic / Q000379:methods*; D014162:Transfection / Q000379:methods
D016704:Synapsins; D049452:Green Fluorescent Proteins