Proteomic analyses of methamphetamine (METH)-induced differential protein expression by immature dendritic cells (IDC).
In the US, the increase in methamphetamine (METH) use has been associated with increased human immunodeficiency virus (HIV-1) infection. Dendritic cells (DC) are the first line of defense against HIV-1. DC play a critical role in harboring HIV-1 and facilitate the infection of neighboring T cells. However, the role of METH on HIV-1 infectivity and the expression of the proteome of immature dendritic cells (IDC) has not been elucidated. We hypothesize that METH modulates the expression of a number of proteins by IDC that foster the immunopathogenesis of HIV-1 infection. We utilized LTR amplification, p24 antigen assay and the proteomic method of difference gel electrophoresis (DIGE) combined with protein identification through high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) to analyze the effects of METH on HIV-1 infectivity (HIV-1 IIIB; CXCR4-tropic, X4 strain) and the proteomic profile of IDC. Our results demonstrate that METH potentiates HIV-1 replication in IDC. Furthermore, METH significantly differentially regulates the expression of several proteins including CXCR3, protein disulfide isomerase, procathepsin B, peroxiredoxin and galectin-1. Identification of unique, METH-induced proteins may help to develop novel markers for diagnostic, preventive and therapeutic targeting in METH using subjects.
Reynolds
Jessica L
JL
Department of Medicine, Division of Allergy, Immunology and Rheumatology, State University of New York at Buffalo, Buffalo General Hospital, Buffalo, NY 14203, USA. jlr8@buffalo.edu
Mahajan
Supriya D
SD
Sykes
Donald E
DE
Schwartz
Stanley A
SA
Nair
Madhavan P N
MP
D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism* / Q000821:virology; D005786:Gene Expression Regulation / Q000187:drug effects; D015497:HIV-1 / Q000502:physiology; D006801:Humans; D008694:Methamphetamine / Q000494:pharmacology*; D010544:Peroxidases / Q000096:biosynthesis; D054464:Peroxiredoxins; D040901:Proteomics / Q000379:methods; D014779:Virus Replication / Q000187:drug effects
D008694:Methamphetamine; D010544:Peroxidases; D054464:Peroxiredoxins