Sensitive and rapid LC-ESI-MS method for the determination of trimetazidine in human plasma.
A sensitive method, based on liquid chromatography-electrospray ionisation-mass spectrometry (LC-ESI-MS), was developed for the determination of trimetazidine in human plasma. Buflomedil was used as the internal standard (IS). Plasma samples were extracted with a mixture of cyclohexane-diethyl ether (1:1, v/v) and the analytes were chromatographically separated on a phenomenex Luna 5 mu C18 (2) 100A HPLC column with a mobile phase of 10 mM ammonium acetate buffer solution containing 0.1% acetic acid-methanol (45:55, v/v). The electrospray ionization was employed in a single quadrupole mass spectrometer for the analytical determination. The lower limit of quantification (LLOQ) was 0.5 ng/ml for trimetazidine and the measuring ranges were from 0.5 to 200 ng/ml. The intra- and inter-run standard deviation was less than 4.1% and 7.8%, respectively. The method was successfully applied to study the pharmacokinetics of trimetazidine in healthy Chinese volunteers.
Ding
Li
L
Department of Pharmaceutical Analysis, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, China. dinglidl@hotmail.com
Gong
Bin
B
Chu
Xiaoxiang
X
Hu
Jingjing
J
Zheng
Hen
H
D000328:Adult; D019540:Area Under Curve; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006207:Half-Life; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions; D021241:Spectrometry, Mass, Electrospray Ionization; D014292:Trimetazidine / Q000097:blood* / Q000493:pharmacokinetics; D014665:Vasodilator Agents / Q000097:blood* / Q000493:pharmacokinetics
D007202:Indicators and Reagents; D012996:Solutions; D014665:Vasodilator Agents; D014292:Trimetazidine