[Identification of fetal nucleated erythrocytes in maternal blood using short tandem repeat typing after primer extension preamplification].
OBJECTIVE
To develop a method for identifying fetal nucleated erythrocytes (NRBCs) in maternal blood.


METHODS
NRBCs in maternal blood were detected by benzidine staining and collected by micromanipulation. After primer extension preamplification (PEP) of the entire genome from a single NRBC, short tandem repeat (STR) genotype was analysed after further amplification of this gene. Single NRBC was differentiated as fetal or maternal origin by comparison of STR genotype of NRBC with its corresponding parents.


RESULTS
NRBCs were found in all of 28 pregnant women in a range of 4 to 13 per 5 ml venous blood. About 43. 6% of NRBCs were determined to be fetal origin by STR typing.


CONCLUSIONS
This method provides effective identification of fetal NRBCs and allows non-invasive prenatal genetic diagnosis using single fetal NRBC.
Wei
Hong-ying
HY
Department of Pediatrics, The First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China.
Long
Gui-fang
GF
Lin
Wei-xiong
WX
Li
Shu-quan
SQ
D000328:Adult; D004900:Erythroblasts; D004906:Erythrocyte Count; D005260:Female; D005333:Fetus / Q000166:cytology; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D020411:Oligonucleotide Array Sequence Analysis; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D017086:beta-Thalassemia / Q000175:diagnosis*