[Biodistribution study of 131I-gM-CSF in SCID mice bearing human leukemia].
OBJECTIVE
To investigate the biodistribution of 131I-GM-CSF in SCID mice bearing human AML in vivo.


METHODS
The xenograft model of human leukemia was established in SCID mice. In the leukemia mice, the biodistribution of 131I-GM-CSF produced by chlo amine-T method was studied.


RESULTS
(1)The inoculated HL-60 cells could grow in SCID mice, which developed leukemia after 4 weeks. (2) 131 I-GM-CSF was concentrated in spleen, bone marrow and tumor tissue of the mice. In spleen and bone marrow, 131 I-GM-CSF was uptaken to peak in 30 minutes after injection, the up taking rate was (442. 9+/-86. 4) % ID/g and (4283. 8+/-252. 8)% ID/g, respectively, and maintained on higher level in 24 hours. The injection of 131I resulted in an even distribution in the whole body.


CONCLUSIONS
131 I-GM-CSF is able to concentrate electively in spleen, bone marrow and organs infiltrated by leukemia cells. The biodistribution of 131I-GM-CSF in the leukemia mice is tissue specific.
Xu
Yi-zhi
YZ
Department of Hematology, the Second College of Clinical Medicine, Chongqing Medical University, Chongqing 400010, China.
Lou
Shi-feng
SF
Deng
Yang-jia
YJ
D000818:Animals; D005260:Female; D005434:Flow Cytometry; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000493:pharmacokinetics*; D018922:HL-60 Cells; D006801:Humans; D007457:Iodine Radioisotopes; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism*; D051379:Mice; D016513:Mice, SCID; D023041:Xenograft Model Antitumor Assays
D007457:Iodine Radioisotopes; D016178:Granulocyte-Macrophage Colony-Stimulating Factor