Overexpression of hUMP1/POMP proteasome accessory protein enhances proteasome-mediated antioxidant defence.
The proteasome is the major cellular proteolytic machinery. It is involved in the regulation of various pathways via the selective degradation of either short-lived normal proteins or damaged proteins permitting the cellular detoxification. Proteasome has impaired function during several biological processes, including aging and diseases; however, it can be activated through overexpression of beta(5)- or beta(1)-subunits, resulting to enhanced survival and extended lifespan. In the current study, we have investigated proteasomal up-regulation via overexpression of hUMP1/POMP protein, the known accessory factor for proteasome assembly in humans. hUMP1/POMP overexpressing fibroblasts have increased levels of functional proteasome and enhanced capacity to cope better and faster with various oxidative stressors. These data highlight hUMP1/POMP role in proteasome assembly and further strengthen the prospect of genetic manipulation of the proteasomal system.
Chondrogianni
Niki
N
National Hellenic Research Foundation, Institute of Biological Research and Biotechnology, 48 Vas. Constantinou Ave., Athens 116 35, Greece.
Gonos
Efstathios S
ES
D015720:5'-Nucleotidase / Q000235:genetics / Q000378:metabolism*; D000975:Antioxidants / Q000378:metabolism*; D002460:Cell Line; D002470:Cell Survival; D002477:Cells / Q000378:metabolism*; D016922:Cellular Senescence; D015180:Electrophoresis, Gel, Two-Dimensional; D005347:Fibroblasts; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D018384:Oxidative Stress; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014162:Transfection / Q000379:methods; D015854:Up-Regulation
D000975:Antioxidants; D006023:Glycoproteins; D017382:Reactive Oxygen Species; D015720:5'-Nucleotidase; C412794:NT5C3A protein, human; D046988:Proteasome Endopeptidase Complex