[Experimental study on dog's bone marrow stem cells transfected by pIRES2-EGFP-IGF-1 gene].
OBJECTIVE
To establish the bone marrow stem cells (MSC) model which could highly express the insulin-like growth factor 1 (IGF-1) transfected by dog's IGF-1 gene.


METHODS
pIRES2-EGFP-IGF-1 was transfected into MSC by lipofectamine. Positive clones were selected with G418. The expression of IGF-1 protein in the MSC was determined by immunohistochemistry and Western blot analysis. The IGF-1 in the supernatant of the transfected MSC was detected by sandwich-in ELISA. The periodontal ligament cells (PDLC) were cultured in the supernatant of the transfected MSC. The changes of PDLC' proliferation were observed by MTT.


RESULTS
IGF-1-transfected MSC could apparently express IGF-1. The IGF-1 protein in the supernatant of the transfected MSC was confirmed by sandwich-in ELISA. IGF-1 could promote the PDLC' proliferation.


CONCLUSIONS
The MSC transfected by dog's IGF-1 gene can highly express IGF-1, which may lay the foundation for further study on periodontal regeneration.
Zhu
Guo-qiang
GQ
Department of Stomatology, The 264th Hospital of PLA, Taiyuan 030001, China.
Wu
Zhi-fen
ZF
Li
Yuan-fei
YF
Hu
De-hua
DH
Wang
Qin-tao
QT
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002478:Cells, Cultured; D004285:Dogs; D005822:Genetic Vectors; D007334:Insulin-Like Growth Factor I / Q000235:genetics* / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D014162:Transfection
D007334:Insulin-Like Growth Factor I