Monitoring BCR-ABL transcript levels by real-time quantitative polymerase chain reaction: a linear regression equation to convert from BCR-ABL/B2M ratio to estimated BCR-ABL/ABL ratio.
In order to overcome the problem of different control genes for BCR-ABL normalization, we used a linear regression equation to compare our results previously obtained using B2M as the control gene with those calculated using the ABL gene and validated the slope as a factor to convert from B2M to ABL results.
Iacobucci
Ilaria
I
Galletti
Livia
L
Amabile
Marilina
M
Soverini
Simona
S
Baccarani
Michele
M
Martinelli
Giovanni
G
D000465:Algorithms; D003199:Computer Systems; D016044:Fusion Proteins, bcr-abl / Q000096:biosynthesis* / Q000235:genetics; D016313:Genes, abl; D006801:Humans; D016014:Linear Models; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics; D012334:RNA, Neoplasm / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics; D001613:beta 2-Microglobulin / Q000235:genetics
D012333:RNA, Messenger; D012334:RNA, Neoplasm; D001613:beta 2-Microglobulin; D016044:Fusion Proteins, bcr-abl