[The regulation effect of interleukin-1 on expression of cartilage specificity gene Sox9 mRNA in the human intervertebral discs].
OBJECTIVE
To assess the regulating effects of interleukin-1 (IL-1) on gene expression of cartilage specificity gene Sox9 and type II collagen mRNA in the human intervertebral discs.


METHODS
RT-PCR were used to investigate the effects of IL-1 on gene expression of Sox9 and type II collagen mRNA in intervertebral discs cells cultures of embryo.


RESULTS
The Sox9 and type II collagen mRNA in intervertebral discs were decreased progressively along with the addition concentrations of IL-1 than the controls. And the mRNA of Sox9 and type II collagen also markedly decreased with the time of culture.


CONCLUSIONS
IL-1 could cause dose-dependent and time-dependent inhibition effects on Sox9 and type II collagen gene expression in human intervertebral discs.
Zhao
Yong
Y
Department of Orthopaedics, the First Affiliated Hospital, Harbin Medical University, Harbin 150001, China.
Wang
Wen-bo
WB
Lu
Yu
Y
Ge
Yun
Y
Yu
Zhan-ge
ZG
Xue
Fei
F
D002478:Cells, Cultured; D024043:Collagen Type II / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects; D006609:High Mobility Group Proteins / Q000235:genetics*; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D007403:Intervertebral Disc / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055758:SOX9 Transcription Factor; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics*
D024043:Collagen Type II; D006609:High Mobility Group Proteins; D007375:Interleukin-1; D012333:RNA, Messenger; D055758:SOX9 Transcription Factor; C527932:SOX9 protein, human; D014157:Transcription Factors