Properties of recombinant glycine decarboxylase P- and H-protein subunits from the cyanobacterium Synechocystis sp. strain PCC 6803.
The multi-enzyme complex glycine decarboxylase is important for one-carbon metabolism, essential for the photorespiratory glycolate cycle of plants, and comprises four different polypeptides, P-, H-, T-, and L-protein. We report on the production and properties of recombinant P-protein from the cyanobacterium Synechocystis and also describe features of recombinant H-protein from the same organism. The P-protein shows enzymatic activity with lipoylated H-protein and very low activity with H-apoprotein or lipoate as artificial cofactors. Its affinity towards glycine is unaffected by the presence and nature of the methyleneamine acceptor molecule. The cyanobacterial H-protein apparently forms stable dimers.
Hasse
Dirk
D
Universität Rostock, Institut für Biowissenschaften, Abteilung Pflanzenphysiologie, Albert-Einstein-Strasse 3, D-18059 Rostock, Germany.
Mikkat
Stefan
S
Thrun
Hans-Albrecht
HA
Hagemann
Martin
M
Bauwe
Hermann
H
D019281:Dimerization; D005998:Glycine / Q000737:chemistry; D050961:Glycine Decarboxylase Complex H-Protein / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D050841:Glycine Dehydrogenase (Decarboxylating) / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D021122:Protein Subunits; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D046939:Synechocystis / Q000201:enzymology*
D050961:Glycine Decarboxylase Complex H-Protein; D021122:Protein Subunits; D011994:Recombinant Proteins; D050841:Glycine Dehydrogenase (Decarboxylating); D005998:Glycine