[Influence of glucose-6-phosphate dehydrogenase on rat adrenal 11-beta-hydroxylase].
Previous work has shown NADPH to be a necessary cofactor in adrenal production of steroids. Synthesis is, in effect, a function of dehydrogenase activity and the adrenals are particularly rich in these enzymes. The present paper deals with an appraisal of the importance of G 6-PD as a source of NADPH for mitochondrial hydroxylation. 11-beta-hydroxylation was used as parameter. It was found that the activity of this enzyme, and hence NAPDH production, was stimulated by the administration of ACTH; no parallel increase in 11-beta-hydroxylase activity was noted.
Lauro
R
R
Istituto di Patologia Speciale Medica e Metodologia Clinica dell'Università di Torino.
Fonzo
D
D
Dogliotti
L
L
De Martinis
C
C
D000302:Adrenal Cortex / Q000187:drug effects / Q000378:metabolism*; D000305:Adrenal Cortex Hormones / Q000096:biosynthesis; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000818:Animals; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000502:physiology*; D006900:Hydroxylation / Q000187:drug effects; D007016:Hypophysectomy; D008928:Mitochondria / Q000378:metabolism; D009249:NADP / Q000096:biosynthesis*; D051381:Rats; D013252:Steroid 11-beta-Hydroxylase / Q000378:metabolism*
D000305:Adrenal Cortex Hormones; D009249:NADP; D000324:Adrenocorticotropic Hormone; D005954:Glucosephosphate Dehydrogenase; D013252:Steroid 11-beta-Hydroxylase