title: CD34(+) progenitors are reproducibly recovered in thawed umbilical grafts, and positively influence haematopoietic reconstitution after transplantation.
abstract: Cord blood (CB) units are increasingly used for allogeneic transplantation. Cell dose, a major factor for CB selection, is evaluated before freezing by each CB bank, using various techniques. This may introduce variability and affect the prediction of cell recovery after thawing, or haematopoietic reconstitution. Forty-two children were transplanted at the same institution with unrelated CB units. All units were thawed and evaluated at the same cell therapy facility, using standard procedures. We investigated: (i) factors that affect cell loss after thawing, and (ii) the importance of CD34(+) cell doses. Prefreeze and post-thaw CD34(+) cell doses were statistically correlated, thus suggesting that variability in numeration techniques used by different CB banks does not compromise the biological and clinical value of these figures. CD34(+) cell recovery appeared to be correlated with the absolute number of CD34(+) cells per frozen bag. Infused CD34(+) is the cell dose that better correlates with platelet reconstitution delay; in addition, when using a quartile comparison, haematopoietic recovery appeared to be related with prefreeze and post-thaw CD34(+) cell doses. We conclude that enumeration of CD34(+) cells in CB units is of biological significance, and may help select CB units and identify patients at risk of delayed recovery.
authors.affiliation: Centre de Thérapie Cellulaire et Génique, Département de Biologie, Institut Paoli-Calmettes, Marseille, France. thercell@marseille.fnclcc.fr
authors.forename: C
authors.initials: C
authors.lastname: Lemarie
authors.forename: B
authors.initials: B
authors.lastname: Esterni
authors.forename: B
authors.initials: B
authors.lastname: Calmels
authors.forename: B
authors.initials: B
authors.lastname: Dazey
authors.forename: V
authors.initials: V
authors.lastname: Lapierre
authors.forename: L
authors.initials: L
authors.lastname: Lecchi
authors.forename: A
authors.initials: A
authors.lastname: Meyer
authors.forename: D
authors.initials: D
authors.lastname: Rea
authors.forename: I
authors.initials: I
authors.lastname: Thuret
authors.forename: H
authors.initials: H
authors.lastname: Chambost
authors.forename: C
authors.initials: C
authors.lastname: Curtillet
authors.forename: C
authors.initials: C
authors.lastname: Chabannon
authors.forename: G
authors.initials: G
authors.lastname: Michel
mesh_terms: D015703:Antigens, CD / Q000097:blood; D018952:Antigens, CD34 / Q000097:blood*; D018929:Cell Culture Techniques / Q000592:standards; D002648:Child; D036101:Cord Blood Stem Cell Transplantation / Q000379:methods* / Q000592:standards; D005312:Fetal Blood; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods* / Q000592:standards; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D007700:Kinetics; D007958:Leukocyte Count; D009369:Neoplasms / Q000628:therapy*; D010976:Platelet Count; D015203:Reproducibility of Results; D012189:Retrospective Studies; D019172:Transplantation Conditioning; D014184:Transplantation, Homologous; D014916:Whole-Body Irradiation
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; D007166:Immunosuppressive Agents