[Study on monocyte HLA-DR expression in critically ill patients after surgery].
OBJECTIVE
To explore the HLA-DR expression on CD14(+) monocyte in peripheral blood of critically ill patients after surgery and observe its relationship with prognosis of patients.


METHODS
HLA-DR expression on CD14(+) monocytes in peripheral blood was measured in critically ill patients after surgery (30 cases) by using flow cytometry on the day 1, 4 and 7 after entered the ICU, and were compared with those in the healthy volunteers (28 cases). APACHE II score, sepsis-related organ failure assessment (SOFA) score, age, sex and 28 d prognosis were recorded.


RESULTS
The HLA-DR expression on CD14(+) monocyte in peripheral blood in critically ill patients after surgery were lower than that of healthy volunteers (P < 0.01). The CD14(+) monocyte HLA-DR expression on day 1 after entered ICU was not correlated with APACHE II score, SOFA and 28 d prognosis. However, compared with those with decreased HLA-DR expression, those with increased monocyte HLA-DR expression on day 7 had a better 28 d survival rate (P < 0.01).


CONCLUSIONS
In critically ill patients after surgery, the decreased HLA-DR expression on CD14(+) monocyte in peripheral blood on day 1 after entered ICU could not be regarded as a prognostic parameter, but it is significative to monitor the increased expression of HLA-DR on CD14(+) monocyte for evaluating the 28 d prognosis.
Zhang
Yun-Tao
YT
The Department of ICU, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.
Fang
Qiang
Q
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016638:Critical Illness; D005260:Female; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000097:blood*; D006801:Humans; D007362:Intensive Care Units; D018950:Lipopolysaccharide Receptors / Q000097:blood; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D011184:Postoperative Period; D011379:Prognosis
D006684:HLA-DR Antigens; D018950:Lipopolysaccharide Receptors