In vivo mutagenesis using EZ-Tn5.
Epicentre Biotechnologies has developed a suite of transposon-based tools for use in modern bacterial genetics. This chapter highlights the EZ-Tn5 Transposome system and focuses on in vivo mutagenesis and subsequent rescue cloning. Many other applications and variations have been described and are available through Epicentre's website at http://www.epibio.com/.
Kirby
John R
JR
School of Biology, Georgia Institute of Technology, Atlanta, GA, USA.
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D016680:Genome, Bacterial; D016254:Mutagenesis, Insertional; D019895:Transposases
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D019895:Transposases