Fungal cell wall polysaccharides isolated from Discula destructiva spp.
The alkali-extractable water-soluble polysaccharides F1SS isolated from the cell wall of four species of Discula destructiva have been studied by methylation analysis and NMR spectroscopy, and their idealized structures established as [structure: see text] where n approximately 2 for strains CBS 109771 and CBS 133.91, n approximately 1 for CBS 132.91, and it has an intermediate value in strain CBS 130.91. The mannan core was obtained by mild hydrolysis of the F1SS polysaccharide and its structure consisted of a skeleton of alpha-(1-->6)-mannopyranan, with around one out of eleven residues substituted at C-2 by short chains (one to six units) of 2-substituted mannopyranoses.
Ahrazem
Oussama
O
Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain.
Prieto
Alicia
A
Leal
Juan Antonio
JA
Inmaculada Giménez-Abián
M
M
Jiménez-Barbero
Jesús
J
Bernabé
Manuel
M
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry*; D005690:Galactose / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000737:chemistry; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D020037:Sordariales / Q000737:chemistry*
D008351:Mannans; D011134:Polysaccharides; D005690:Galactose