Accumulation and depuration of cyanobacterial toxin nodularin and biomarker responses in the mussel Mytilus edulis.
Blue mussels (Mytilus edulis) were exposed to an extract made of natural cyanobacterial mixture containing toxic cyanobacterium Nodularia spumigena (70-110 microg nodularin l(-1), 24-h exposure followed by 144-h depuration period in clean water). Toxin concentration increased from initial 400 to 1100 mg kg(-1) after 24-h exposure, measured by liquid chromatography/mass spectrometry (LC/MS). Acetylcholinesterase activity (AChE), a biomarker of direct neurotoxic effects, showed inhibition after 12 and 24h exposure but returned to control level during the depuration period. Catalase (CAT) activity, an indicator of oxidative stress, showed significantly elevated levels in exposed mussels but only 72 h after the end of the exposure. No change in the activity of glutathione-S-transferase (GST) involved in conjugation reactions could be observed. A gradual yet incomplete elimination of nodularin (from 1100 to 600 mg kg(-1)) was observed during the depuration period, and the tissue levels were 30% lower in clean water after 24 h. The observed increase in oxidative stress indicated by elevated CAT activity is likely connected to detoxification reactions leading to the production of reactive oxygen species, including an apparent time lag in this specific enzymatic defence response. That no change in GST activity was observed suggests that this enzyme is not significantly involved in the detoxification process of nodularin-containing cyanobacterial extract in M. edulis.
Kankaanpää
Harri
H
Finnish Institute of Marine Research, Erik Palménin aukio 1, FI-00561 Helsinki, Finland. kankaanpaa@fimr.fi
Leiniö
Sari
S
Olin
Miikka
M
Sjövall
Olli
O
Meriluoto
Jussi
J
Lehtonen
Kari K
KK
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001427:Bacterial Toxins / Q000378:metabolism* / Q000494:pharmacology; D015415:Biomarkers / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D002853:Chromatography, Liquid; D000458:Cyanobacteria / Q000378:metabolism; D000087522:Cyanobacteria Toxins; D004789:Enzyme Activation / Q000187:drug effects; D005880:Gills / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008387:Marine Toxins / Q000378:metabolism* / Q000494:pharmacology; D013058:Mass Spectrometry; D052998:Microcystins / Q000378:metabolism* / Q000494:pharmacology; D049880:Mytilus edulis / Q000187:drug effects / Q000201:enzymology*; D010456:Peptides, Cyclic / Q000378:metabolism* / Q000494:pharmacology; D013997:Time Factors
D001427:Bacterial Toxins; D015415:Biomarkers; D002800:Cholinesterase Inhibitors; D000087522:Cyanobacteria Toxins; D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; C063998:nodularin; D002374:Catalase; D005982:Glutathione Transferase; D000110:Acetylcholinesterase