Sodium butyrate: a chemical inducer of in vivo reactivation of herpes simplex virus type 1 in the ocular mouse model.
Recent studies have explored the chromatin structures associated with the herpes simplex virus type 1 (HSV-1) genome during latency, particularly with regard to specific histone tail modifications such as acetylation and dimethylation. The objective of our present study was to develop a rapid systemic method of in vivo HSV-1 reactivation to further explore the changes that occur in the chromatin structures associated with HSV-1 at early time points after the initiation of HSV reactivation. We present a uniform, rapid, and reliable method of in vivo HSV-1 reactivation in mice that yields high reactivation frequencies (75 to 100%) by using sodium butyrate, a histone deacetylase inhibitor, and demonstrate that the reactivating virus can be detected at the original site of infection.
Neumann
Donna M
DM
Department of Ophthalmology (LSU Eye Center of Excellence), Louisiana State University Health Sciences Center at New Orleans, New Orleans, Louisiana 70112, USA.
Bhattacharjee
Partha S
PS
Hill
James M
JM
D000818:Animals; D002087:Butyrates / Q000494:pharmacology*; D004195:Disease Models, Animal; D005260:Female; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000502:physiology*; D016849:Keratitis, Herpetic / Q000821:virology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010865:Pilot Projects; D011817:Rabbits; D012964:Sodium / Q000494:pharmacology; D014775:Virus Activation / Q000187:drug effects* / Q000502:physiology*; D017735:Virus Latency / Q000187:drug effects / Q000502:physiology
D002087:Butyrates; D012964:Sodium