title: Maternal and embryonic sources of tyrosine hydroxylase during Drosophila embryogenesis.
abstract: Tyrosine hydroxylase (TH), the enzyme which catalyzes the conversion of tyrosine to L-DOPA and is rate limiting in catecholamine biosynthesis, is biochemically expressed in late stage wild-type Drosophila oocytes as well as in early embryogenesis. Null mutant alleles of TH (pale) are embryonic lethals with death occurring in the late embryonic or early larval periods of development. Staging of embryos demonstrated that inhibition of the enzymatic activity of TH by alpha-methyl-p-tyrosine (alphaMT) retards the progression of embryos primarily during the organogenesis stages of embryonic development, with lesser effects on earlier and later stages. On the other hand, time of gene action studies with a conditional temperature sensitive pale mutant (ple(ts1)) at its restrictive temperature (29 degrees C) indicate an onset of tyrosine hydroxylase gene action beginning in the oocyte stage of development. Thus, maternal as well as embryonic effects on the secretion and/or functionality of this enzyme may play roles in the early developmental program of the organism.
authors.forename: Robert
authors.initials: R
authors.lastname: Pendleton
authors.forename: Adrian
authors.initials: A
authors.lastname: Alday
authors.forename: Prasuna
authors.initials: P
authors.lastname: Paluru
authors.forename: Jeannettee
authors.initials: J
authors.lastname: Joyner
authors.forename: Natalie
authors.initials: N
authors.lastname: Jerome
authors.forename: Ralph
authors.initials: R
authors.lastname: Hillman
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000196:embryology / Q000201:enzymology*; D004625:Embryo, Nonmammalian / Q000201:enzymology* / Q000502:physiology; D047108:Embryonic Development; D005260:Female; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D009865:Oocytes / Q000201:enzymology; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics / Q000378:metabolism*
chemical_list: D014446:Tyrosine 3-Monooxygenase