title: Upregulation of IGF-I in the goldfish retinal ganglion cells during the early stage of optic nerve regeneration.
abstract: Goldfish retinal ganglion cells (RGCs) can regrow their axons after optic nerve injury. However, the reason why goldfish RGCs can regenerate after nerve injury is largely unknown at the molecular level. To investigate regenerative properties of goldfish RGCs, we divided the RGC regeneration process into two components: (1) RGC survival, and (2) axonal elongation processes. To characterize the RGC survival signaling pathway after optic nerve injury, we investigated cell survival/death signals such as Bcl-2 family members in the goldfish retina. Amounts of phospho-Akt (p-Akt) and phospho-Bad (p-Bad) in the goldfish retina rapidly increased four- to five-fold at the protein level by 3-5 days after nerve injury. Subsequently, Bcl-2 levels increased 1.7-fold, accompanied by a slight reduction in caspase-3 activity 10-20 days after injury. Furthermore, level of insulin-like growth factor-I (IGF-I), which activates the phosphatidyl inositol-3-kinase (PI3K)/Akt system, increased 2-3 days earlier than that of p-Akt in the goldfish retina. The cellular localization of these molecular changes was limited to RGCs. IGF-I treatment significantly induced phosphorylation of Akt, and strikingly induced neurite outgrowth in the goldfish retina in vitro. On the contrary, addition of the PI3K inhibitor wortmannin, and IGF-I antibody inhibited Akt phosphorylation and neurite outgrowth in an explant culture. Thus, we demonstrated, for the first time, the signal cascade for early upregulation of IGF-I, leading to RGC survival and axonal regeneration in adult goldfish retinas through PI3K/Akt system after optic nerve injury. The present data strongly indicate that IGF-I is one of the most important molecules for controlling regeneration of RGCs after optic nerve injury.
authors.affiliation: Department of Molecular Neurobiology, Graduate School of Medicine, Kanazawa University, Kanazawa 920-8640, Japan.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Koriyama
authors.forename: Keiko
authors.initials: K
authors.lastname: Homma
authors.forename: Kayo
authors.initials: K
authors.lastname: Sugitani
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Higuchi
authors.forename: Toru
authors.initials: T
authors.lastname: Matsukawa
authors.forename: Daisuke
authors.initials: D
authors.lastname: Murayama
authors.forename: Satoru
authors.initials: S
authors.lastname: Kato
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology; D002470:Cell Survival; D006054:Goldfish; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D009409:Nerve Crush; D009416:Nerve Regeneration / Q000502:physiology*; D016501:Neurites / Q000378:metabolism; D051058:Oncogene Protein v-akt / Q000378:metabolism; D020221:Optic Nerve Injuries; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D012333:RNA, Messenger; D012160:Retina / Q000166:cytology; D012165:Retinal Ganglion Cells / Q000502:physiology*; D015854:Up-Regulation
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I; D051058:Oncogene Protein v-akt