Use of colorimetric method for evaluation of LPS of different structure.
We studied the possibility of using the colorimetric method with carbocyanin dye for quantitative measurements of LPS of different structure in aqueous solutions. The absorption spectra (intensity and position of absorption peaks) of LPS-carbocyanin complexes depend not only on the presence of polysaccharide fragment in LPS structure, but also on the core structure.
Zubova
S V
SV
Laboratory of Molecular Biomedicine, Institute of Fundamental Problems of Biology, Russian Academy of Sciences, Pushchino. zusvet@rambler.ru
Prokhorenko
I R
IR
D002232:Carbocyanines; D003124:Colorimetry / Q000379:methods*; D048168:Escherichia coli K12 / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D013054:Spectrophotometry, Atomic
D002232:Carbocyanines; D008070:Lipopolysaccharides