title: High performance liquid chromatographic determination of 3-methylflavone-8-carboxylic acid, the main active metabolite of flavoxate hydrochloride in human urine.
abstract: High performance liquid chromatographic (HPLC) method was presented for the determination of 3-methylflavone-8-carboxylic acid as the main active metabolite of flavoxate hydrochloride (FX) in human urine. The proposed method was based on using CN column with mobile phase consisting of acetonitrile-12 mM ammonium acetate (40:60, v/v) and adjusted to apparent pH 4.0 with flow rate of 1.5 ml min(-1). Quantitation was achieved with UV detection at 220 nm. The proposed method was utilized to the determination of dissolution rate for tablets containing flavoxate hydrochloride. The urinary excretion pattern has been calculated using the proposed method.
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt. ghhadad@hotmail.com
authors.forename: Alaa
authors.initials: A
authors.lastname: El-Gindy
authors.forename: Shehab
authors.initials: S
authors.lastname: Sallam
authors.forename: Randa A
authors.initials: RA
authors.lastname: Abdel-Salam
mesh_terms: D000085:Acetates / Q000737:chemistry; D000097:Acetonitriles / Q000737:chemistry; D000328:Adult; D002138:Calibration; D002264:Carboxylic Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004355:Drug Stability; D004562:Electrocardiography; D005422:Flavoxate / Q000032:analysis / Q000378:metabolism / Q000652:urine; D006801:Humans; D006851:Hydrochloric Acid / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007668:Kidney / Q000502:physiology; D008099:Liver / Q000502:physiology; D008297:Male; D010276:Parasympatholytics / Q000032:analysis / Q000378:metabolism / Q000652:urine; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012972:Sodium Hydroxide / Q000737:chemistry; D012995:Solubility; D013010:Sonication; D013056:Spectrophotometry, Ultraviolet; D013607:Tablets / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
chemical_list: D000085:Acetates; D000097:Acetonitriles; D002264:Carboxylic Acids; D010276:Parasympatholytics; D013607:Tablets; D005422:Flavoxate; D012972:Sodium Hydroxide; D006851:Hydrochloric Acid; C018824:ammonium acetate; C032159:acetonitrile