A new method for express analysis of flavonoid fraction in nephrophite plant extract and prediction of its antioxidant effects.
A new chromatographic procedure is proposed for rapid (35 min) complete fractionation of flavonoids in plant raw material; the method well discriminates between components with antioxidant activity (rutin, apigenin, quercetin, hesperidin). The method is based on reverse-phase high performance liquid chromatography using ODS-S5CN carrier and linear 10-60% pyridine elution gradient.
Markaryan
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I. M. Setchenov Moscow Medical Academy, Moscow. markaryan@mmascience.ru.
D000975:Antioxidants / Q000378:metabolism*; D047310:Apigenin / Q000032:analysis; D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005419:Flavonoids / Q000302:isolation & purification* / Q000378:metabolism; D006569:Hesperidin / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry*; D011794:Quercetin / Q000032:analysis; D012431:Rutin / Q000032:analysis
D000975:Antioxidants; D005419:Flavonoids; D010936:Plant Extracts; C497518:nephrophyt; D012431:Rutin; D047310:Apigenin; D011794:Quercetin; D006569:Hesperidin