title: Association between duodenal contents reflux and squamous cell carcinoma--establishment of an esophageal cancer cell line derived from the metastatic tumor in a rat reflux model.
abstract: Squamous cell carcinoma (SCC) of the human esophagus has a multifactorial etiology involving several environmental and/or genetic factors. Recently, gastroesophageal reflux has been implicated as a causative factor in upper aerodigestive tract carcinogenesis. The development of esophageal squamous cell carcinoma (ESCC) in a duodenal contents reflux model without any known carcinogen present has been reported previously. In this study, the duodenal contents reflux model without gastrectomy was used. At 60 weeks post-operatively, all surviving animals had malignant lesions as follows: ESCC (40%), esophageal adenocarcinoma (EAC) (20%) and adenosquamous carcinoma (40%). In one subject, a well-differentiated ESCC was detected with thoracic dissemination and metastases in lymph nodes. A novel cell line, designated ESCC-DR, was established from the thoracic metastatic tumor at the 60th post-operative week. These cells were transplanted into nude mice, and the developed nodules represented a well differentiated ESCC, resembling that of the parent site. Duodenal contents reflux has a great potential for malignant initiation and plays a role in developing not only EAC but also ESCC.
authors.affiliation: Department of Pathology, Shiga University of Medical Science, Seta-tsukinowa-cho, Ohtsu, Shiga 520-2192, Japan.
authors.forename: Kuan-Hao
authors.initials: KH
authors.lastname: Chen
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Mukaisho
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Ling
authors.forename: Akihiko
authors.initials: A
authors.lastname: Shimomura
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sugihara
authors.forename: Takanori
authors.initials: T
authors.lastname: Hattori
mesh_terms: D000818:Animals; D001471:Barrett Esophagus / Q000150:complications / Q000209:etiology / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000209:etiology* / Q000473:pathology; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D004383:Duodenogastric Reflux / Q000150:complications*; D004938:Esophageal Neoplasms / Q000209:etiology* / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D051381:Rats; D017208:Rats, Wistar; D013899:Thoracic Neoplasms / Q000473:pathology / Q000556:secondary*