title: Site specific phosphorylation of cytochrome c oxidase subunits I, IVi1 and Vb in rabbit hearts subjected to ischemia/reperfusion.
abstract: We have mapped the sites of ischemia/reperfusion-induced phosphorylation of cytochrome c oxidase (CcO) subunits in rabbit hearts by using a combination of Blue Native gel/Tricine gel electrophoresis and nano-LC-MS/MS approaches. We used precursor ion scanning combined with neutral loss scanning and found that mature CcO subunit I was phosphorylated at tandem Ser115/Ser116 positions, subunit IVi1 at Thr52 and subunit Vb at Ser40. These sites are highly conserved in mammalian species. Molecular modeling suggests that phosphorylation sites of subunit I face the inter membrane space while those of subunits IVi1 and Vb face the matrix side.
authors.affiliation: Laboratory of Biochemistry, The Department of Animal Biology, School of Veterinary Medicine, 3800 Spruce Street, University of Pennsylvania, Philadelphia, PA 19104, United States.
authors.forename: Ji-Kang
authors.initials: JK
authors.lastname: Fang
authors.forename: Subbuswamy K
authors.initials: SK
authors.lastname: Prabu
authors.forename: Naresh B
authors.initials: NB
authors.lastname: Sepuri
authors.forename: Haider
authors.initials: H
authors.lastname: Raza
authors.forename: Hindupur K
authors.initials: HK
authors.lastname: Anandatheerthavarada
authors.forename: Domenico
authors.initials: D
authors.lastname: Galati
authors.forename: Joseph
authors.initials: J
authors.lastname: Spear
authors.forename: Narayan G
authors.initials: NG
authors.lastname: Avadhani
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002853:Chromatography, Liquid; D003576:Electron Transport Complex IV / Q000737:chemistry* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005998:Glycine / Q000031:analogs & derivatives / Q000737:chemistry; D006321:Heart; D008969:Molecular Sequence Data; D015428:Myocardial Reperfusion Injury / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology*; D010766:Phosphorylation; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D011817:Rabbits; D012694:Serine / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D013912:Threonine / Q000378:metabolism
chemical_list: D021122:Protein Subunits; D013912:Threonine; D012694:Serine; D003576:Electron Transport Complex IV; D005998:Glycine; C100184:tricine