Differential expression of two vasa/PL10-related genes during gametogenesis in the special model system Rana.
Germline cell fate decisions are primarily controlled at the post-transcriptional level with DEAD-box RNA helicases playing a crucial role in germline development. In this study, we report the identification of two DEAD-box vasa/PL10 orthologues (RlVlg and RlPL10) in a species complex of the genus Rana, characterized by hybridogenetic reproduction, an enigmatic process that involves the exclusion of an individual genome, and endoreduplication events. Both genes were expressed during the early stages of gametogenesis of R. ridibunda, R. lessonae, and their natural hybrid R. esculenta. RlVlg expression was germline specific. On the other hand, RlPL10 was also expressed in somatic tissues, although only at low levels. The two genes were expressed in different phases of mitotic and meiotic spermatogenetic divisions as demonstrated by immunostaining with an anti-H3 phosphohistone antibody. The data indicate that RlVlg and RlPL10 may represent useful markers for dissecting the molecular aspects of genome exclusion and endoreduplication of the hybridogenetic gametogenesis.
Marracci
Silvia
S
Laboratorio di Biologia Cellulare e dello Sviluppo, Dipartimento di Biologia, Università di Pisa, Via Carducci 13, 56010 Ghezzano, Pisa, Italy, smarracci@biologia.unipi.it
Casola
Claudio
C
Bucci
Stefania
S
Ragghianti
Matilde
M
Ogielska
Maria
M
Mancino
Giorgio
G
D000595:Amino Acid Sequence; D000818:Animals; D053487:DEAD-box RNA Helicases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005718:Gametogenesis / Q000235:genetics*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D008297:Male; D008969:Molecular Sequence Data; D009866:Oogenesis / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011898:Ranidae / Q000235:genetics*; D013091:Spermatogenesis / Q000235:genetics; D013737:Testis / Q000166:cytology / Q000378:metabolism
D012333:RNA, Messenger; D053487:DEAD-box RNA Helicases