title: Bioactive surface modification of mica and poly(dimethylsiloxane) with hydrophobins for protein immobilization.
abstract: Bioactive surfaces with appropriate hydrophilicity for protein immobilization can be achieved by hydrophobin II (HFBI) self-assembly on mica and polydimethylsiloxane (PDMS) surfaces. X-ray photoelectron spectroscopy and water contact angle measurements illustrated that the surface wettability can be changed from superhydrophobic (PDMS) or superhydrophilic (mica) to moderately hydrophilic, which is suitable for protein (chicken IgG) immobilization on both substrate surfaces. The results suggest that HFBI assembly, one kind of hydrophobin from Trichoderma reesei, may be a versatile and convenient method for the immobilization of biomolecules on diverse substrates, which may have potential applications in biosensors, immunoassays, and microfluidic networks.
authors.affiliation: The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin 300071, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Qin
authors.forename: Li-Kai
authors.initials: LK
authors.lastname: Wang
authors.forename: Xi-Zeng
authors.initials: XZ
authors.lastname: Feng
authors.forename: Yan-Lian
authors.initials: YL
authors.lastname: Yang
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Yu
authors.forename: Bin
authors.initials: B
authors.lastname: Shao
authors.forename: Ming-Qiang
authors.initials: MQ
authors.lastname: Qiao
mesh_terms: D000538:Aluminum Silicates / Q000737:chemistry*; D000818:Animals; D002627:Chemistry, Physical / Q000379:methods; D002645:Chickens; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000737:chemistry; D044085:Microfluidics; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D011506:Proteins / Q000737:chemistry*; D012828:Silicones / Q000737:chemistry*; D013052:Spectrometry, X-Ray Emission; D013499:Surface Properties; D014242:Trichoderma / Q000378:metabolism; D014867:Water / Q000737:chemistry
chemical_list: D000538:Aluminum Silicates; D004129:Dimethylpolysiloxanes; D007074:Immunoglobulin G; D011506:Proteins; D012828:Silicones; D014867:Water; C013830:baysilon; C011934:mica