title: Construction of the bifunctional enzyme cellulase-beta-glucosidase from the hyperthermophilic bacterium Thermotoga maritima.
abstract: An artificial bifunctional enzyme, cellulase-beta-glucosidase, was prepared by gene fusion from the hyperthermophilic bacterium Thermotoga maritima MSB8. The fusion protein exhibited both cellulase (Cel5C) and beta-glucosidase (BglB) activity when the bglB gene was fused to downstream of cel5C, but not when cel5C was fused to downstream of bglB. The specific activity of the bifunctional enzyme was 70% lower than that of cellulase or beta-glucosidase. The fusion enzyme was purified, and the MW was estimated as 114 kDa. The fusion enzyme displayed optimum cellulase activity at pH 8.0 and 70 degrees C over 30 min, and optimal beta-glucosidase activity at pH 7.0 and 80 degrees C over 30 min.
authors.affiliation: Division of Applied Life Science, Gyeongsang National University, Chinju, Korea.
authors.forename: Su-Young
authors.initials: SY
authors.lastname: Hong
authors.forename: Jin-Suk
authors.initials: JS
authors.lastname: Lee
authors.forename: Kye-Man
authors.initials: KM
authors.lastname: Cho
authors.forename: Renukaradhya K
authors.initials: RK
authors.lastname: Math
authors.forename: Yong-Hee
authors.initials: YH
authors.lastname: Kim
authors.forename: Sun-Joo
authors.initials: SJ
authors.lastname: Hong
authors.forename: Yong-Un
authors.initials: YU
authors.lastname: Cho
authors.forename: Soo-Jeong
authors.initials: SJ
authors.lastname: Cho
authors.forename: Hoon
authors.initials: H
authors.lastname: Kim
authors.forename: Han-Dae
authors.initials: HD
authors.lastname: Yun
mesh_terms: D002480:Cellulase / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D008957:Models, Genetic; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D013696:Temperature; D020124:Thermotoga maritima / Q000201:enzymology* / Q000235:genetics; D001617:beta-Glucosidase / Q000235:genetics / Q000378:metabolism*
chemical_list: D011993:Recombinant Fusion Proteins; D001617:beta-Glucosidase; D002480:Cellulase