title: Two conditional media promoting the differentiation murine embryonic stem cells into hematopoietic stem cells.
abstract: OBJECTIVE
  To derive hematopoietic stem cells with functional properties of hematopoietic reconstitution from murine embryonic stem (ES) cells.
  
  
  METHODS
  ES-D3 cells by formation of the day-4 embryoid bodies (4dEBs) were induced into hematopoietic stem cells by co-culture with murine bone marrow endothelial cell-conditional medium (mBMEC-CM) and the fetal liver stromal cell-conditional medium (FLSC-CM). This experiment was designed to 4 groups (mBMEC-CM + FLSC-CM group, mBMEC-CM group, FLSC-CM group, and the control group).
  
  
  RESULTS
  The total cell numbers, CD34+ cell numbers, and colony numbers formed in the mBMEC-CM+FLSC-CM group were the highest among the 4 groups. The cells in the mBMEC-CM + FLSC-CM group resumed the hematopoietic system of the mice after being transplanted with the inducing cells.
  
  
  CONCLUSIONS
  The culture condition combing mBMEC-CM with FLSC-CM can promote murine ES cells differentiating into hematopoietic stem cells with functional properties of hematopoietic reconstitution.
authors.affiliation: Institute of Human Reproductive and Stem Cell Engineering, Central South University, National Human Stem Cell Engineering Research Center, Changsha 410078, China.
authors.forename: Hui-Ping
authors.initials: HP
authors.lastname: Zhao
authors.forename: Guang-Xiu
authors.initials: GX
authors.lastname: Lu
authors.forename: Qi-Ru
authors.initials: QR
authors.lastname: Wang
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000032:analysis; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology; D005260:Female; D005434:Flow Cytometry; D006410:Hematopoiesis; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011833:Radiation Injuries, Experimental / Q000601:surgery
chemical_list: D018952:Antigens, CD34; D017077:Culture Media, Conditioned