Relaxation of protons by radicals in rotationally immobilized proteins.
Proton spin-lattice relaxation by paramagnetic centers may be dramatically enhanced if the paramagnetic center is rotationally immobilized in the magnetic field. The details of the relaxation mechanism are different from those appropriate to solutions of paramagnetic relaxation agents. We report here large enhancements in the proton spin-lattice relaxation rate constants associated with organic radicals when the radical system is rigidly connected with a rotationally immobilized macromolecular matrix such as a dry protein or a cross-linked protein gel. The paramagnetic contribution to the protein-proton population is direct and distributed internally among the protein protons by efficient spin diffusion. In the case of a cross-linked-protein gel, the paramagnetic effects are carried to the water spins indirectly by chemical exchange mechanisms involving water molecule exchange with rare long-lived water molecule binding sites on the immobilized protein and proton exchange. The dramatic increase in the efficiency of spin relaxation by organic radicals compared with metal systems at low magnetic field strengths results because the electron relaxation time of the radical is orders of magnitude larger than that for metal systems. This gain in relaxation efficiency provides completely new opportunities for the design of spin-lattice relaxation based contrast agents in magnetic imaging and also provides new ways to examine intramolecular protein dynamics.
Korb
Jean-Pierre
JP
Physique de la Matière Condensée, Ecole Polytechnique, CNRS, 91128 Palaiseau, France.
Diakova
Galina
G
Goddard
Yanina
Y
Bryant
Robert G
RG
D000818:Animals; D002417:Cattle; D005609:Free Radicals / Q000737:chemistry; D008956:Models, Chemical; D011522:Protons; D012399:Rotation; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D005609:Free Radicals; D011522:Protons; D014867:Water; D012710:Serum Albumin, Bovine