title: NT-proBNP changes, oxidative stress, and energy status of hypertrophic myocardium following ischemia/reperfusion injury.
abstract: N-terminal pro-B-type natriuretic peptide (NT-proBNP) is a sensitive functional marker in heart disease, including left ventricular hypertrophy (LVH) secondary to valvular aortic stenosis (AS). We evaluated the association between NT-proBNP changes, oxidative stress, energy status and severity of LVH in patients with AS. Ten patients undergoing aortic valve replacement for AS were studied. Plasma NT-proBNP concentrations were performed by electroluminescence immunoassay 15min after the induction of anesthesia (t0), before aortic cross-clamping (t1), before clamp removal (t2), 15min after myocardial reperfusion (t3), and 24h after surgery (t4). Heart biopsies were obtained and high energy phosphates (ATP, ADP, AMP) were analyzed by capillary electrophoresis (CE). In plasma samples from the coronary sinus, nitrate plus nitrite (NOx) concentrations were also analyzed by CE. Echocardiographic measurements were acquired and correlations between biochemical markers and severity of AS were assessed. NT-proBNP peaked significantly at t4 (p<0.001). A linear correlation between NT-proBNP values measured at t0 and t4 was found (R(2)=0.89; p<0.001). A negative correlation between NT-proBNP production and phosphorylation potential (ATP/ADP ratio) was observed (R(2)=0.62; p<0.01). NOx values positively correlated with NT-proBNP levels (p<0.01). NT-proBNP inversely correlated with aortic valvular area (r=81, p<0.01), positively correlated with mean (r=0.82, p<0.01) and maximum left ventricle-to-aortic gradients (r=0.80, p<0.01), and with left ventricular mass (r=0.69, p<0.01). NT-proBNP is a useful marker of LVH and severity of AS. It may complement echocardiographic evaluation of patients with AS in identifying the optimum time for surgery.
authors.affiliation: Dipartimento di Chirurgia e Bioingegneria, Università di Siena, Policlinico S. Maria alle Scotte, Viale Bracci 1, 53100 Siena, Italy. scolletta@unisi.it
authors.forename: S
authors.initials: S
authors.lastname: Scolletta
authors.forename: F
authors.initials: F
authors.lastname: Carlucci
authors.forename: B
authors.initials: B
authors.lastname: Biagioli
authors.forename: L
authors.initials: L
authors.lastname: Marchetti
authors.forename: M
authors.initials: M
authors.lastname: Maccherini
authors.forename: G
authors.initials: G
authors.lastname: Carlucci
authors.forename: F
authors.initials: F
authors.lastname: Rosi
authors.forename: M
authors.initials: M
authors.lastname: Salvi
authors.forename: A
authors.initials: A
authors.lastname: Tabucchi
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000368:Aged; D000369:Aged, 80 and over; D001024:Aortic Valve Stenosis / Q000503:physiopathology* / Q000601:surgery; D015415:Biomarkers / Q000097:blood; D002312:Cardiomyopathy, Hypertrophic / Q000503:physiopathology*; D019075:Electrophoresis, Capillary; D004734:Energy Metabolism; D005112:Extracorporeal Circulation; D005260:Female; D006324:Heart Arrest, Induced; D019918:Heart Valve Prosthesis Implantation; D006801:Humans; D017379:Hypertrophy, Left Ventricular / Q000378:metabolism / Q000503:physiopathology*; D007118:Immunoassay; D008163:Luminescent Measurements; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000503:physiopathology*; D020097:Natriuretic Peptide, Brain / Q000097:blood*; D009566:Nitrates / Q000097:blood; D009573:Nitrites / Q000097:blood; D018384:Oxidative Stress; D010446:Peptide Fragments / Q000097:blood*; D010766:Phosphorylation; D012720:Severity of Illness Index
chemical_list: D015415:Biomarkers; D009566:Nitrates; D009573:Nitrites; D010446:Peptide Fragments; C109794:pro-brain natriuretic peptide (1-76); D020097:Natriuretic Peptide, Brain; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate