Anti-inflammatory effect of Sasim extracts in PHA-stimulated THP-1 and peripheral blood mononuclear cells from cerebral infarction patients.
Sasim, a prescription composed of seven herbal mixtures, has been widely used for the treatment of cerebral infarction as an oriental medicine in Korea. However, the mechanisms by which the formula affects on the production of pro-inflammatory cytokines in cerebral infarct patients remain unknown yet. The levels of secretory protein and mRNA of pro-inflammatory cytokines, including tumor necrosis factor (TNF)-alpha, interlukin (IL)-1beta, and IL-6, were significantly increased in both THP-1 differentiated macrophage-like cells (T/M) and peripheral blood mononuclear cells (PBMCs) from cerebral infarct patients at 24h after stimulation with phytohemagglutinin (PHA) (p<0.05). However, pretreatment of Sasim strongly suppressed the secretion of pro-inflammatory cytokines in PHA-stimulated T/M cells and PBMCs. Moreover, Sasim significantly suppressed the transcriptional levels of pro-inflammatory cytokines in PHA-stimulated THP-1/M cells. These data indicate that Sasim may be beneficial in the cessation of inflammatory processes of cerebral infarction through suppression on the production of pro-inflammatory cytokines via inhibition of mRNA expression.
Kim
Yunha
Y
Vestibulocochlear System Research Center and Department of Microbiology, Wonkwang University School of Medicine, Iksan, Jeonbuk 570-749, Republic of Korea.
So
Hong-Seob
HS
Youn
Myung-Ja
MJ
Kim
Hyung-Jin
HJ
Woo
Won-Hong
WH
Shin
Sun-Ho
SH
Lee
In
I
Moon
Byung-Soon
BS
Cho
Kwang-Ho
KH
Park
Raekil
R
D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D002544:Cerebral Infarction / Q000097:blood*; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D066298:In Vitro Techniques; D007723:Korea; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008262:Macrophage Activation; D008518:Medicine, East Asian Traditional; D009000:Monocytes / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000893:Anti-Inflammatory Agents; D016207:Cytokines; D010835:Phytohemagglutinins; D010936:Plant Extracts; D012333:RNA, Messenger; C522580:Sasim