title: SCCmec typing and detection of VISA-related genes in methicillin-resistant Staphylococcus aureus clinical strains from Kobe University Hospital, Japan.
abstract: A total of 50 clinical strains of methicillin-resistant Staphylococcus aureus (MRSA) were collected from Kobe University Hospital in 2003. Molecular typing of SCCmec was performed by multiplex polymerase chain reaction (PCR) and the presence of six genes (vraR, vraG, vraA, vraF, fruA, and fruB) associated with vancomycin (VCM) resistance was examined by simple PCR analysis. Out of 50 MRSA strains isolated 47 strains contained Type II SCCmec and the remaining contained Type IV SCCmec. Thirty seven strains contained pUB110 plasmid. VraA was present in 69% of the strains, vraF in 10%, vraG in 53%, and vraR in 16%. Noteworthy, strains without pUB110 contained vraR in relatively higher frequency (31%) compared with strains with pUB110 (11%).
authors.affiliation: International Center for Medical Research and Treatment, Kobe University School of Medicine, Japan.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Takata
authors.forename: Toshiro
authors.initials: T
authors.lastname: Shirakawa
authors.forename: Jun
authors.initials: J
authors.lastname: Ito
authors.forename: Asako
authors.initials: A
authors.lastname: Okamoto
authors.forename: Muh Nasrum
authors.initials: MN
authors.lastname: Massi
authors.forename: Shohiro
authors.initials: S
authors.lastname: Kinoshita
authors.forename: Mochammad
authors.initials: M
authors.lastname: Hatta
authors.forename: Masato
authors.initials: M
authors.lastname: Kawabata
mesh_terms: D001426:Bacterial Proteins / Q000187:drug effects / Q000235:genetics; D015373:Bacterial Typing Techniques; D002876:Chromosomes, Bacterial; D006785:Hospitals, University; D006801:Humans; D007564:Japan; D008712:Methicillin / Q000494:pharmacology; D016106:Methicillin Resistance / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D046915:Penicillin-Binding Proteins; D016133:Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology* / Q000517:prevention & control; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D046915:Penicillin-Binding Proteins; C487789:mecA protein, Staphylococcus aureus; D008712:Methicillin