title: Analysis of fluctuation in protein abundance without promoter regulation based on Escherichia coli continuous culture.
abstract: Fluctuation of protein abundance of isogenic Escherichia coli cells in uniform environment was studied. Based on a continuous culture system, which provides homogeneous culture environment, we investigated the fluctuation in GlnA protein abundance regardless of known glnALG promoter regulation. As results by flow cytometer, we found that the GlnA protein abundance in the cells exhibit a large fluctuation, even though GlnA protein is an essential factor for cell growth and the environment is homogeneous. Furthermore, among several steady states, transient processes of such heterogeneous cell population were investigated, by changing the environmental conditions. The results showed that the expression of GlnA protein can be controlled, depending on its necessity, even though there is no known regulatory machinery. These results may provide a clue to understand the nature of regulation of protein expression dynamics with the stochastic fluctuation.
authors.affiliation: Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yamada
authors.forename: Chikara
authors.initials: C
authors.lastname: Furusawa
authors.forename: Keisuke
authors.initials: K
authors.lastname: Nagahisa
authors.forename: Akiko
authors.initials: A
authors.lastname: Kashiwagi
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Yomo
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shimizu
mesh_terms: D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism*; D005434:Flow Cytometry; D005798:Genes, Bacterial; D005974:Glutamate-Ammonia Ligase / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D011401:Promoter Regions, Genetic; D013269:Stochastic Processes; D049490:Systems Biology
chemical_list: D029968:Escherichia coli Proteins; C081173:glutamine synthetase I; D005974:Glutamate-Ammonia Ligase