title: Kaurane diterpenoids from Isodon excisus inhibit LPS-induced NF-kappaB activation and NO production in macrophage RAW264.7 cells.
abstract: As part of an ongoing search for plant-derived compounds that inhibit the activation of NF-kappaB, the methanol extract of the aerial parts of Isodon excisus was found to have significant inhibitory effects on the activation of NF-kappaB in murine macrophage RAW264.7 cells. Bioactivity-guided isolation of the extract yielded five new diterpenoids, excisusin A-E (1-5), along with seven known compounds, inflexarabdonin I (6), inflexarabdonin G (7), inflexin (8), inflexanin A (9), inflexanin B (10), inflexinol (11), and inflexarabdonin A (12). The structures were determined by analysis of the spectroscopic data including 2D NMR. All of the isolates were evaluated for their inhibitory effects on LPS-induced NF-kappaB activation and nitric oxide production in RAW264.7 cells.
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk 361-763, Korea, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yuseong, Daejeon 305-600, Korea.
authors.forename: Seong Su
authors.initials: SS
authors.lastname: Hong
authors.forename: Seon A
authors.initials: SA
authors.lastname: Lee
authors.forename: Xiang Hua
authors.initials: XH
authors.lastname: Han
authors.forename: Hui Zi
authors.initials: HZ
authors.lastname: Jin
authors.forename: Jeong Hyung
authors.initials: JH
authors.lastname: Lee
authors.forename: Dongho
authors.initials: D
authors.lastname: Lee
authors.forename: Jung Joon
authors.initials: JJ
authors.lastname: Lee
authors.forename: Jin Tae
authors.initials: JT
authors.lastname: Hong
authors.forename: Youngsoo
authors.initials: Y
authors.lastname: Kim
authors.forename: Jai Seup
authors.initials: JS
authors.lastname: Ro
authors.forename: Bang Yeon
authors.initials: BY
authors.lastname: Hwang
mesh_terms: D000818:Animals; D045786:Diterpenes, Kaurane / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D037602:Isodon / Q000737:chemistry*; D007723:Korea; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D008968:Molecular Conformation; D015394:Molecular Structure; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D009569:Nitric Oxide / Q000037:antagonists & inhibitors*; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D045786:Diterpenes, Kaurane; D008070:Lipopolysaccharides; D016328:NF-kappa B; C583089:inflexanin B; D009569:Nitric Oxide