title: [Expression of bcl-2 gene in EBV-transformed human gastric epithelial cell line GES-1].
abstract: OBJECTIVE
  To detect bcl-2 gene expression in Epstein-Barr virus (EBV)-infected human gastric epithelial cell line GES-1 for understanding the role of bcl-2 gene in the carcinogenesis of EBV-associated gastric carcinoma.
  
  
  METHODS
  Akata 1061 cells producing recombined EBV carrying neomycin resistance gene (NEOr) was used to mediate the EBV infection of human gastric epithelial cell line GES-1 via close contact, with the empty plasmid pcDNA3-transfected GES-1 cells via lipofectamine method as a control. The EBV-infected and pcDNA3-transfected cells were cloned by limited dilution and the positive clones selected with G418. Immunocytochemical staining was performed to detect the expressions of EBNA1 and Bcl-2 protein.
  
  
  RESULTS
  Bcl-2 protein expression was detected in EBV-infected cells but not in the control cells.
  
  
  CONCLUSIONS
  EBV infection can increase Bcl-2 expression in gastric epithelial cells, and such cell transformation effect of EBV is related to the overexpression of bcl-2 gene.
authors.affiliation: Department of Biology, North China Coal Medical College, Tangshan 063000, China. zhulihua1972@163.com
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Zhu
authors.forename: Tian-Ji
authors.initials: TJ
authors.lastname: Zhou
authors.forename: Guo-You
authors.initials: GY
authors.lastname: Shi
authors.forename: Guang-Ling
authors.initials: GL
authors.lastname: Zhang
authors.forename: Shu-Ying
authors.initials: SY
authors.lastname: Li
mesh_terms: D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism* / Q000821:virology; D004854:Herpesvirus 4, Human; D006801:Humans; D007150:Immunohistochemistry; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis*; D013270:Stomach / Q000166:cytology
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2