title: [Alpha,beta-poly[(N-hydroxypropyl/aminoethyl)-DL-aspartamide -co-L-lysine]: potential non-viral vehicle for gene delivery].
abstract: A series of Poly[aspartic acid-co-L-lysine](PAL) are copolycondensed by DL-aspartice acid and L-lysine with different ratios. Their constructions are identified by the spectra of 1H-NMR, FT-IR, X-Ray). These spectra are proved to have good regularity of these copolymers. alpha,beta-Poly[(N-hydroxypropyl/aminoethyl)-DL-Aspartamide-co-L-lysine] (PHAAL) is synthesized by ring-opening poly [aspartic acid-co-lysine] (PAL). PHAAL has good degradability in the phosphoric acid buffer solution (0.01 M, pH = 7.4) in the enzyme solution (Papain, Trypsine). PHAAL appeared tobe low cytotoxicity in Hela, ECV-304, Bcap37 cell lines, which was quantified by MTT assay. The combination ability of PHAAL with plasmid DNA was evaluated by agarose gel electrophoresis with agarose gel (1.0% w/v) containing ethidium bromide (0.25 microg/ml). The PHAAL with higher ratios of lysine in the copolymers have higher ability of condensing DNA. In summary, PHAAL, the polyaminoacid materials, could be one kind of macromolecule materials tobeused as the non-viral gene vehicle.
authors.affiliation: Hangzhou First People's Hospital, Hangzhou 310006, China. adoer@sina.com
authors.forename: Yin
authors.initials: Y
authors.lastname: Luo
authors.forename: Si'en
authors.initials: S
authors.lastname: Hou
authors.forename: Zhongming
authors.initials: Z
authors.lastname: Gao
authors.forename: Guping
authors.initials: G
authors.lastname: Tang
mesh_terms: D001224:Aspartic Acid / Q000138:chemical synthesis* / Q000737:chemistry / Q000633:toxicity; D001704:Biopolymers / Q000737:chemistry / Q000633:toxicity; D042783:Endothelial Cells / Q000166:cytology; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000138:chemical synthesis / Q000737:chemistry / Q000633:toxicity; D006367:HeLa Cells; D006801:Humans; D008422:Materials Testing; D000091402:Prohibitins; D014470:Umbilical Cord / Q000166:cytology
chemical_list: D001704:Biopolymers; C000717331:PHB2 protein, human; D000091402:Prohibitins; C499773:alpha,beta-poly(aspartic acid); D001224:Aspartic Acid