title: Evaluation of man-tailored cellulose-based carriers in glucoamylase immobilization.
abstract: Covalent immobilization of glucoamylase on the cellulose-based carrier Granocel was optimized by changing the anchor groups and the methods of activation/immobilization. Binding of the enzyme was via its primary amino groups. It was shown that using carbodiimide and divinyl sulfone for the activation of -COOH and -OH groups on the carrier resulted in the preparations with very low activity. A third method, using pentaethylenehexamine with glutaraldehyde, led to the attachment through a long spacer arm and to the preparations with the highest activity. Further optimization of the carrier's structure consisted of changing pore diameters and amount of functional groups on the carrier surface. The highest activity of bound glucoamylase was obtained by linking the protein via glutaraldehyde on NH(2)-Granocel having high pore size and high number of functional groups. The immobilized enzyme was stable throughout extended storage and possessed higher thermal stability.
authors.affiliation: Faculty of Chemistry, Wrocław University of Technology, Wybrzeze Wyspiańskiego 27, 50-373 Wrocław, Poland. jolanta.bryjak@pwr.wroc.pl
authors.forename: Jolanta
authors.initials: J
authors.lastname: Bryjak
authors.forename: Jolita
authors.initials: J
authors.lastname: Aniulyte
authors.forename: Jolanta
authors.initials: J
authors.lastname: Liesiene
mesh_terms: D002482:Cellulose / Q000032:analysis / Q000737:chemistry*; D004337:Drug Carriers; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D013213:Starch / Q000378:metabolism*; D013816:Thermodynamics
chemical_list: D004337:Drug Carriers; D004800:Enzymes, Immobilized; D002482:Cellulose; D013213:Starch; D005087:Glucan 1,4-alpha-Glucosidase