title: Transmural variations in gene expression of stretch-modulated proteins in the rat left ventricle.
abstract: The properties of left ventricular cardiac myocytes vary transmurally. This may be related to the gradients of stress and strain experienced in vivo across the ventricular wall. We tested the hypothesis that within the rat left ventricle there are transmural differences in the expression of genes for proteins that are involved in mechanosensitive pathways and in associated physiological responses. Real time reverse transcription polymerase chain reaction was used to measure messenger RNA (mRNA) levels of selected targets in sub-epicardial (EPI) and sub-endocardial (ENDO) myocardium. Carbon fibres were attached to single myocytes to stretch them and to record contractility. We observed that the slow positive inotropic response to stretch was not different between EPI and ENDO myocytes and consistent with this, that the mRNA expression of two proteins implicated in the slow response, non-specific cationic mechanosensitive channels (TRPC-1) and Na/H exchanger, were not different. However, mRNA levels of other targets, e.g. the mechanosensitive K(+) channel TREK-1, Brain Natriuretic Peptide and Endothelin-1 receptor B, were significantly greater in ENDO than EPI. No targets had significantly greater mRNA levels in EPI than ENDO. On the basis of these findings, we suggest that the response of the ventricle to stretch will depend upon both the regional differences in stimuli and the relative expression of the mechanosensitive targets and that generally, stretch sensitivity is predicted to be greater in ENDO.
authors.affiliation: Institute of Membrane and Systems Biology, University of Leeds, Worsley Building, Leeds, UK.
authors.forename: R
authors.initials: R
authors.lastname: Stones
authors.forename: S C
authors.initials: SC
authors.lastname: Calaghan
authors.forename: R
authors.initials: R
authors.lastname: Billeter
authors.forename: S M
authors.initials: SM
authors.lastname: Harrison
authors.forename: E
authors.initials: E
authors.lastname: White
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation; D006352:Heart Ventricles / Q000166:cytology / Q000378:metabolism*; D040542:Mechanotransduction, Cellular / Q000502:physiology; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism; D020097:Natriuretic Peptide, Brain / Q000235:genetics / Q000378:metabolism*; D024683:Potassium Channels, Tandem Pore Domain / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D044023:Receptor, Endothelin B / Q000235:genetics / Q000378:metabolism*; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics / Q000378:metabolism; D050052:TRPC Cation Channels / Q000235:genetics / Q000378:metabolism
chemical_list: D024683:Potassium Channels, Tandem Pore Domain; D012333:RNA, Messenger; D044023:Receptor, Endothelin B; D017923:Sodium-Hydrogen Exchangers; D050052:TRPC Cation Channels; C103742:potassium channel protein TREK-1; C096351:transient receptor potential cation channel, subfamily C, member 1; D020097:Natriuretic Peptide, Brain