Production of mouse embryoid bodies with hepatic differentiation potential by stirred tank bioreactor.
Embryonic stem (ES) cells can differentiate into functional hepatic lineage cells, which can potentially be used in biomedicine. To obtain hepatic lineage cells from ES cells, embryoid bodies (EBs) must be formed. In this study, we developed an EB formation system using a spinner flask for mass production of EBs. ES cells were inoculated into the spinner flask, where they formed EBs within 4 d. The EBs were then transferred into an attached culture for hepatic differentiation. To verify the hepatic lineage cells, albumin secretion and hepatic-specific gene expression were examined. We found that EBs formed by either the spinner flask or hanging drops exhibited similar albumin secretion potential and hepatic-specific gene expression. We conclude that the spinner flask method can be used to produce mouse EBs that can be used to mass produce hepatic lineage cells for use in biomedicine.
Yin
Chih-Hsiu
CH
Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan.
Chen
Wannhsin
W
Hsiao
Chang-Chun
CC
Kuo
Chao-Ying
CY
Chen
Chao-Long
CL
Wu
Wen-Teng
WT
D000418:Albumins / Q000378:metabolism; D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D019149:Bioreactors; D002454:Cell Differentiation / Q000502:physiology*; D019070:Cell Lineage; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology*; D022781:Hepatocytes / Q000166:cytology* / Q000378:metabolism; D051379:Mice
D000418:Albumins; D000943:Antigens, Differentiation