title: Genetic differentiation of the giant honey bee (Apis dorsata) in Thailand analyzed by mitochondrial genes and microsatellites.
abstract: Genetic diversity and population differentiation of the giant honey bee (Apis dorsata) in Thailand were examined. Six PCR-RFLP mitotypes were generated from digestion of the COI-COII, Cytb-tRNA(ser), ATPase6-8, and lrRNA genes with Dra I and Hin fI. Low genetic diversity (h=0.074, pi=0.032%) and a lack of genetic population differentiation between A. dorsata originating from geographically different regions were observed from mtDNA polymorphisms (P > 0.05). In contrast, microsatellite (A14, A24, and A88) polymorphisms revealed a relatively high level of genetic diversity in A. dorsata (H (o)=0.68-0.74, average number of alleles per locus=6.0-9.0). Both A24 and A88 indicated significant population differentiation between bees from the north-to-central region (north, northeast, and central regions), peninsular Thailand, and Samui Island.
authors.affiliation: Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: S
authors.initials: S
authors.lastname: Insuan
authors.forename: S
authors.initials: S
authors.lastname: Deowanish
authors.forename: S
authors.initials: S
authors.lastname: Klinbunga
authors.forename: S
authors.initials: S
authors.lastname: Sittipraneed
authors.forename: H A
authors.initials: HA
authors.lastname: Sylvester
authors.forename: S
authors.initials: S
authors.lastname: Wongsiri
mesh_terms: D000818:Animals; D001516:Bees / Q000235:genetics*; D005787:Gene Frequency; D017344:Genes, Insect; D050259:Genes, Mitochondrial / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics*; D005843:Geography; D018895:Microsatellite Repeats / Q000235:genetics*; D010802:Phylogeny; D015183:Restriction Mapping; D013785:Thailand