Evaluation of recombinant HP6-Tsag, an 18 kDa Taenia saginata oncospheral adhesion protein, for the diagnosis of cysticercosis.
With the objective of providing inexpensive and reproducible assays for the detection of antibodies indicating exposure to Taenia saginata and Taenia solium, we have evaluated the diagnostic utility of the T. saginata oncosphere adhesion protein (HP6-Tsag), expressed in baculovirus (HP6-Bac) and bacteria (HP6-GST [glutathione S-transferase]), employing enzyme-linked immunosorbent assays (ELISAs) and sera from T. saginata infected cattle, T. solium infected pigs and serum and cerebrospinal fluid (CSF) samples from clinically defined T. solium neurocysticercosis (NCC) patients. The two recombinant proteins were antigenic in all three systems, with the signal to background ratio of the HP6-Bac ELISA slightly higher than that for the HP6-GST ELISA. Assay performance in cattle was similar to previously described peptide-based ELISA assays, although NCC sample sensitivity/specificity was marginally better. The sensitivity of the HP6-Bac and HP6-GST ELISAs was close for active human NCC (77.4 and 80.6% for serum and 76.9 and 73.1% for CSF samples, respectively). In inactive human NCC, however, the sensitivity of the HP6-Bac ELISA was almost twice that of the HP6-GST ELISA. Because peptides are relatively expensive and recombinant proteins are simple and economical to produce, the latter may provide useful reagents for antibody detection in countries with endemic cysticercosis/NCC.
Ferrer
Elizabeth
E
Instituto de Salud Carlos III, Centro Nacional de Microbiología, 28220 Majadahonda, Madrid, Spain.
González
Luís Miguel
LM
Martínez-Escribano
José Angel
JA
González-Barderas
María Eugenia
ME
Cortéz
María Milagros
MM
Dávila
Iris
I
Harrison
Leslie J S
LJ
Parkhouse
R Michael E
RM
Gárate
Teresa
T
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D016367:Baculoviridae / Q000235:genetics / Q000378:metabolism; D001769:Blood / Q000469:parasitology; D002417:Cattle / Q000469:parasitology; D002418:Cattle Diseases / Q000175:diagnosis* / Q000469:parasitology; D002555:Cerebrospinal Fluid / Q000469:parasitology; D003551:Cysticercosis / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D020019:Neurocysticercosis / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology; D011994:Recombinant Proteins / Q000276:immunology; D013552:Swine / Q000469:parasitology; D013553:Swine Diseases / Q000175:diagnosis* / Q000469:parasitology; D041202:Taenia saginata / Q000235:genetics / Q000276:immunology*; D041201:Taenia solium / Q000276:immunology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; C464926:HP6 protein, Taenia saginata; D011994:Recombinant Proteins