[Detection of heterogeneous type II collagen transplantation mediated cytotoxic response in vitro].
To detect the cellular immunity state of New Zealand white rabbit immunized by pig type II collagen. The New Zealand white rabbit was immunized by type II collagen for sixty days. The plasma was collected at a regular interval and the anti-type II collagen antibodies were examined. At the sixtieth day, the peripheral circular lymphocytes and the lymphocytes separated from spleen cells of rabbit and lymph nodes were collected and were stimulated by type II collagen in vivo again. The regulation of reactive cellular proliferation caused by the stimulation was detected. The experiment samples were divided into two groups. The first group was the positive control group by adding different concentrations of PHA and the non-specific immunity was assayed. The different concentrations of type II collagen were added to the second group and the specific immunity was assayed. The lymphocytes of normal rabbits showed proliferation by PHA stimulation but no proliferation by the first stimulation of type II collagen. Obvious proliferation due to the stimulation of both PHA and type II collagen in the immunized rabbit were observed. It shows that certain concentration of heterogeneous collagen may cause an increase of anti-type II collagen antibody in immunized rabbit and may cause a proliferation of lymphocytes in rabbit spleen and peripheral blood. The heterogeneous type II collagen causes cellular immunity in vivo.
Shen
Yan
Y
Medical Research Institute of Traumatic Surgery, Red Cross Hospital of Guangzhou, Guangzhou 510220, China. ann8cn@yahoo.com.cn
Tang
Yi
Y
Zhong
Cancan
C
Liang
Peihong
P
Huang
Xuefang
X
Zhou
Haiyan
H
Chen
Honghui
H
Liang
Weiguo
W
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D024043:Collagen Type II / Q000008:administration & dosage / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D005260:Female; D006649:Histocompatibility Antigens; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology*; D008297:Male; D011817:Rabbits; D013154:Spleen / Q000166:cytology; D013552:Swine; D014183:Transplantation, Heterologous
D024043:Collagen Type II; D006649:Histocompatibility Antigens