Discrimination of C57BL/6J Rj and 129S2/SvPasCrl inbred mouse strains by use of simple sequence length polymorphisms.
We present a list of simple sequence length polymorphisms (SSLPs) that was used in a polymerase chain reaction (PCR)-based marker-assisted selection protocol to facilitate the generation of a C57BL/6J Rj congenic strain from a mixed 129S2/SvPasCrlxC57BL/6J Rj background. We chose informative SSLP markers that would permit evaluation of the PCR products on NuSieve agarose or nondenaturing polyacrylamide gels. This list of oligonucleotide pairs is useful for analyzing and backcrossing knockout or transgenic strains derived from 129S2/SvPasCrl or C57BL/6J Rj backgrounds.
Lamacchia
Celine
C
Department of Pathology and Immunology, University of Geneva School of Mediicne, and Department of Internal Medicine, University Hospital, Switzerland.
Palmer
Gaby
G
Gabay
Cem
C
D000818:Animals; D017931:DNA Primers; D051379:Mice; D020297:Mice, Congenic / Q000235:genetics*; D008810:Mice, Inbred C57BL / Q000235:genetics*; D008815:Mice, Inbred Strains / Q000145:classification* / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D017931:DNA Primers