[The effect of morin on activation, proliferation and cell-cycle of murine T lymphocytes in vitro].
OBJECTIVE
To discover the effects of morin on the activation, proliferation and cell-cycle of murine T lymphocytes in vitro.


METHODS
Murine lymph node-derived T lymphocytes were separated and stimulated with concanavalin A (ConA) and different experimental groups were set by co-cultured with morin of different final concentration. Flow cytometry (FCM) was used to detect the activation, proliferation [carboxylfluorescein diacetate, succinimide ester (CFDA-SE) staining] and cell-cycle [propidium iodide(PI) staining] of T cells.


RESULTS
After 6 h time of culture in vitro, the rate of CD69(+) T cells in control group was (2.97+/-0.12)%, while it was significant higher in ConA group [(72.52+/-0.66)% (P<0.01)]. Morin could down-regulate this rate at final concentration being 25, 50 and 100 micromol/L, with a peak at 100 micromol/L morin [(48.95+/-0.81)% (P<0.01)]. CFDA-SE staining showed that at 48 h and 72 h, the proliferation indexes (PI) of T cells in ConA group were (1.58+/-0.04) and (1.95+/-0.02), respectively. Morin could significantly decrease the PI value at all experimental concentration, with the peak effect at 100 micromol/L morin, which the PI for 48 h was (1.02+/-0.02) and (1.03+/-0.01) for 72 h (P<0.01). FCM analysis of PI staining implied that the percentage of S phase cells in ConA group was (27.05+/-0.39)%, significantly higher than that in control group (5.10+/-0.07)%; and the 25 and 50 micromol/L morin groups showed higher S phase cell rates.


CONCLUSIONS
Morin can significantly inhibit ConA stimulated activation and proliferation of murine T lymphocytes, in which the S phase lagging may serve as one of the major mechanisms.
Zang
Ning
N
Institute of Tissue Transplantation and Immunology, College of Life Science and Technology, Jinan University, Guangzhou, China. ningzang2004@tom.com
Zeng
Yao-Ying
YY
Huang
Xiu-Yan
XY
Wang
Tong
T
Ye
Xue-Yi
XY
Zhou
Jian-Guo
JG
Wang
Hui-Ying
HY
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology*; D005434:Flow Cytometry; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008934:Mitogens / Q000494:pharmacology; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D047309:Flavones
D000975:Antioxidants; D003208:Concanavalin A; D005419:Flavonoids; D008934:Mitogens; C008548:morin; D047309:Flavones