title: Modifications in postprandial triglyceride-rich lipoprotein composition and size after the intake of pomace olive oil.
abstract: OBJECTIVE
  This study was designed to determine the composition of postprandial triglyceride-rich lipoproteins (TRL) after the intake of pomace olive oil (POO), which is a subproduct of the extraction of virgin olive oil (VOO) and presents a high concentration of minor components with biological activity.
  
  
  METHODS
  Meals enriched in POO and refined olive oil (ROO) were administrated to 9 healthy young men and blood was extracted every hour during a postprandial period of 7 hours. Serum and TRL lipid composition were measured by enzymatic and chromatographic methods and apolipoprotein B composition by SDS-PAGE.
  
  
  RESULTS
  POO and ROO showed a very similar fatty acid composition but differed in their unsaponifiable fraction. The content of phytosterols, tocopherols, terpenic acids and alcohols and fatty alcohols was much higher in POO than in ROO. Serum lipids were not affected by the administration of the oils but the triglyceride concentration in TRL and the size of the particles (p < 0.05) after POO was higher at time point 2 h and lower at time point 4 h compared with ROO. In contrast, the number of TRL particles was lower after POO, although the rate of clearance was similar.
  
  
  CONCLUSIONS
  We suggest that the unsaponifiable fraction between the two olive oils affect the size and composition of postprandial TRL, which might have a relevant impact on their atherogenicity.
authors.affiliation: Nutrición y Metabolismo Lipídico, Instituto de la Grasa (CSIC), Av. Padre García Tejero 4, 41012 Seville, SPAIN.
authors.forename: Rosana
authors.initials: R
authors.lastname: Cabello-Moruno
authors.forename: Javier S
authors.initials: JS
authors.lastname: Perona
authors.forename: Jesus
authors.initials: J
authors.lastname: Osada
authors.forename: Maria
authors.initials: M
authors.lastname: Garcia
authors.forename: Valentina
authors.initials: V
authors.lastname: Ruiz-Gutierrez
mesh_terms: D000328:Adult; D001055:Apolipoproteins B / Q000737:chemistry*; D019540:Area Under Curve; D001682:Biological Availability; D018592:Cross-Over Studies; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008074:Lipoproteins / Q000737:chemistry*; D008297:Male; D000069463:Olive Oil; D010316:Particle Size; D010938:Plant Oils / Q000737:chemistry / Q000493:pharmacokinetics; D019518:Postprandial Period / Q000502:physiology; D014280:Triglycerides / Q000032:analysis*
chemical_list: D001055:Apolipoproteins B; D008074:Lipoproteins; D000069463:Olive Oil; D010938:Plant Oils; D014280:Triglycerides