title: PON1 status in haemodialysis patients and the impact of hepatitis C infection.
abstract: OBJECTIVE
  Paraoxonase-1 (PON1) activity has been reported to decrease in both haemodialysis patients and patients with HCV infection. We aimed to investigate paraoxonase and arylesterase activities, and lipid hydroperoxide levels (LOOH) in haemodialysis patients with or without hepatitis C infection, and to find out whether PON1 activity is affected further by the presence of HCV infection in HD patients.
  
  
  METHODS
  Twenty HCV (+) haemodialysis patients, 26 HCV (-) haemodialysis patients, and 26 controls were enrolled. Paraoxonase and arylesterase activities were measured spectrophotometrically. LOOH levels were measured by ferrous oxidation with xylenol orange assay.
  
  
  RESULTS
  Haemodialysis patients with or without HCV infection had lower paraoxonase and arylesterase activities than controls (all p<0.001), while higher LOOH levels (both p<0.001). Paraoxonase and arylesterase activities, and LOOH levels were comparable between haemodialysis patients with or without HCV infection (p>0.05). Significant inverse correlation was observed between paraoxonase or arylesterase activities, and LOOH levels (p<0.05, beta=-0.319 and p<0.05, beta=-0.348, respectively).
  
  
  CONCLUSIONS
  We concluded that PON1 activity significantly decreases in both haemodialysis patients with or without HCV infection. Nevertheless, PON1 activity is not affected further by the presence of HCV infection in haemodialysis patients.
authors.affiliation: Harran University School of Medicine, Department of Internal Medicine, Sanliurfa, Turkey. mehmethoroz@yahoo.com
authors.forename: Mehmet
authors.initials: M
authors.lastname: Horoz
authors.forename: Mehmet
authors.initials: M
authors.lastname: Aslan
authors.forename: Sahbettin
authors.initials: S
authors.lastname: Selek
authors.forename: Ahmet O
authors.initials: AO
authors.lastname: Koylu
authors.forename: Cengiz
authors.initials: C
authors.lastname: Bolukbas
authors.forename: Filiz F
authors.initials: FF
authors.lastname: Bolukbas
authors.forename: Hakim
authors.initials: H
authors.lastname: Celik
authors.forename: Ozcan
authors.initials: O
authors.lastname: Erel
mesh_terms: D000328:Adult; D043303:Aryldialkylphosphatase / Q000097:blood*; D002265:Carboxylic Ester Hydrolases / Q000097:blood; D005260:Female; D019698:Hepatitis C, Chronic / Q000150:complications / Q000201:enzymology*; D006801:Humans; D007676:Kidney Failure, Chronic / Q000150:complications / Q000201:enzymology*; D008054:Lipid Peroxides / Q000097:blood; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis
chemical_list: D008054:Lipid Peroxides; D002265:Carboxylic Ester Hydrolases; C019734:arylesterase; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human