Bacterial degradation of N,N-diethyl-m-toluamide (DEET): cloning and heterologous expression of DEET hydrolase.
Pseudomonas putida DTB grew aerobically with N,N-diethyl-m-toluamide (DEET) as a sole carbon source, initially breaking it down into 3-methylbenzoate and diethylamine. The former was further metabolized via 3-methylcatechol and meta ring cleavage. A gene from DTB, dthA, was heterologously expressed and shown to encode the ability to hydrolyze DEET into 3-methylbenzoate and diethylamine.
Rivera-Cancel
Giomar
G
Department of Microbiology, Cornell University, Ithaca, NY 14853, USA.
Bocioaga
Daniela
D
Hay
Anthony G
AG
D000595:Amino Acid Sequence; D001483:Base Sequence; D001673:Biodegradation, Environmental; D003001:Cloning, Molecular; D019295:Computational Biology; D003671:DEET / Q000378:metabolism*; D017931:DNA Primers; D006867:Hydrolases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009518:New York; D016958:Pseudomonas putida / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology
D017931:DNA Primers; D012722:Sewage; D003671:DEET; D006867:Hydrolases