title: [Molecular typing of Salmonella paratyphi A isolates from four provinces with pulse-field gel electrophoresis].
abstract: OBJECTIVE
  To analyze the molecular types of Salmonella paratyphi A strains isolated in the recent years, and to construct the standard S. paratyphi A databank in the laboratory surveillance network PulseNet China.
  
  
  METHODS
  S. paratyphi A isolates from 4 provinces were analyzed with the standard pulsed-field gel electrophoresis (PFGE) protocol used in PulseNet and their patterns compared. The databank was constructed with BioNumerics.
  
  
  RESULTS
  Eleven PFGE patterns were obtained, in which 3 predominant patterns were identifies with a similarity coefficient of 96.3%. The strains of these patterns, accounted for 86.5% of the analyzed strains, appeared in different provinces and years.
  
  
  CONCLUSIONS
  The databank of S. paratyphi A was constructed and could be used in laboratory surveillance of S. paratyphi A in PulseNet China. From the analyses on molecular typing of the isolates, data suggested that the predominant strains might cause the epidemics in different regions.
authors.affiliation: State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Zhi-gang
authors.initials: ZG
authors.lastname: Cui
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhang
authors.forename: Ke-cheng
authors.initials: KC
authors.lastname: Tian
authors.forename: Hao-jie
authors.initials: HJ
authors.lastname: Zhong
authors.forename: Feng-cai
authors.initials: FC
authors.lastname: Zhu
authors.forename: Wei-li
authors.initials: WL
authors.lastname: Liang
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Zhang
authors.forename: Biao
authors.initials: B
authors.lastname: Kan
mesh_terms: D002681:China; D016521:Electrophoresis, Gel, Pulsed-Field; D012482:Salmonella paratyphi A / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification