[Genetic identification of Acanthamoeba sp. CJY/S1 and CJY/S2 isolated from soil].
Two isolates of Acanthamoeba sp. CJY/SI and CJY/S2 were received from soil in Yanji of Jilin Province. Full 18S rDNA gene was amplified using PCR, cloned and sequenced. The results were analyzed by software Clustal X. The full length of CJY/S1 and CJY/S2 is 2255 bp and 2252 bp respectively, both belong to T4 genotype.
Zheng
Shan-zi
SZ
Department of Immunology and Pathogen Biology, Yanbian University, College of Basic Medicine, Yanji 133000, China.
Xuan
Ying-hua
YH
Wang
Yue-hua
YH
Shen
Cheng-hua
CH
Cui
Chun-quan
CQ
D000048:Acanthamoeba / Q000145:classification / Q000235:genetics*; D000818:Animals; D016000:Cluster Analysis; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D021521:Ribotyping; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity
D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S