Production of D-arabitol by a newly isolated Zygosaccharomyces rouxii.
A newly isolated Zygosaccharomyces rouxii NRRL 27,624 produced D-arabitol as the main metabolic product from glucose. In addition, it also produced ethanol and glycerol. The optimal conditions were temperature 30 degrees C, pH 5.0, 350 rpm, and 5% inoculum. The yeast produced 83.4 +/- 1.1 g D-arabitol from 175 +/- 1.1 g glucose per liter at pH 5.0, 30 degrees C, and 350 rpm in 240 h with a yield of 0.48 g/g glucose. It also produced D-arabitol from fructose, galactose, and mannose. The yeast produced D-arabitol and xylitol from xylose and also from a mixture of xylose and xylulose. Resting yeast cells produced 63.6 +/- 1.9 g D-arabitol from 175 +/- 1.8 g glucose per liter in 210 h at pH 5.0, 30 degrees C and 350 rpm with a yield of 0.36 g/g glucose. The yeast has potential to be used for production of xylitol from glucose via D-arabitol route.
Saha
Badal C
BC
Fermentation Biotechnology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research service, United States Department of Agriculture, Peoria, IL 61604, USA. sahabc@ncaur.usda.gov
Sakakibara
Yoshikiyo
Y
Cotta
Michael A
MA
D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D013402:Sugar Alcohols / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D014993:Xylitol / Q000378:metabolism; D020068:Zygosaccharomyces / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*
D013402:Sugar Alcohols; D005947:Glucose; D014993:Xylitol; C014999:arabitol