Creating recombination-activated genes and sequence-defined mutant libraries using transposons.
The properties of a collection of transposon Tn5 derivatives that generate reporter gene fusions and internal protein tags are summarized. Procedures utilizing several of the transposons for generating genes activated by Cre-loxP recombination and for creating large sequence-defined mutant libraries are described in detail.
Gallagher
Larry
L
Department of Genome Sciences, University of Washington, Seattle, WA, USA.
Turner
Cheri
C
Ramage
Elizabeth
E
Manoil
Colin
C
D001483:Base Sequence; D004251:DNA Transposable Elements; D015723:Gene Library; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D011995:Recombination, Genetic
D004251:DNA Transposable Elements