title: Quantitative detection of protein expression in single cells using droplet microfluidics.
abstract: We demonstrate that single cells can be controllably compartmentalized within aqueous microdroplets; using such an approach we perform high-throughput screening by detecting the expression of a fluorescent protein in individual cells with simultaneous measurement of droplet size and cell occupancy.
authors.affiliation: Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK.
authors.forename: A
authors.initials: A
authors.lastname: Huebner
authors.forename: M
authors.initials: M
authors.lastname: Srisa-Art
authors.forename: D
authors.initials: D
authors.lastname: Holt
authors.forename: C
authors.initials: C
authors.lastname: Abell
authors.forename: F
authors.initials: F
authors.lastname: Hollfelder
authors.forename: A J
authors.initials: AJ
authors.lastname: deMello
authors.forename: J B
authors.initials: JB
authors.lastname: Edel
mesh_terms: D005453:Fluorescence; D044085:Microfluidics / Q000379:methods*; D011506:Proteins / Q000032:analysis*
chemical_list: D011506:Proteins