title: [Cloning, expression and identification of human interleukin-10 gene].
abstract: OBJECTIVE
  To construct a prokaryotic expression vector containing human interleukin-10 (IL-10) gene, express and identify the protein.
  
  
  METHODS
  Human IL-10 gene from HepG2 was isolated and identified by DNA sequencing, and then cloned into the expression vector PET-28 a (+) to construct prokaryotic expression vector. The recombinant protein was expressed in BL21(DE3) and identified by Western blot and ELISA.
  
  
  RESULTS
  The expressed protein was mainly located in the inclusion body. The relative molecular mass of the expressed product was identical to that of prediction. The expressed protein had binding activity with specific antibody.
  
  
  CONCLUSIONS
  The human IL-10 gene was successfully cloned and expressed in E.coli. The expressed product had antigenicity which provides foundation for preparation of monoclonal antibodies against IL-10.
authors.affiliation: Department of Immunology, Beijing Institute of Infections Diseases, Beijing, China. energysj@yeah.net
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
authors.forename: Bo-An
authors.initials: BA
authors.lastname: Li
authors.forename: Yang
authors.initials: Y
authors.lastname: Qi
authors.forename: Ji-Wen
authors.initials: JW
authors.lastname: Zhou
authors.forename: Lei
authors.initials: L
authors.lastname: Zhu
authors.forename: Shu-Ping
authors.initials: SP
authors.lastname: Chi
authors.forename: Yun
authors.initials: Y
authors.lastname: Cheng
mesh_terms: D015153:Blotting, Western; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics* / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D016753:Interleukin-10