[The method of inducing and establishing human pancreatic cancer cell sublines with radiation resistance].
OBJECTIVE
To explore the method of inducing and building pancreatic cancer cell sublines with radiation resistance.


METHODS
Simulating the clinical radiotherapy, the pancreatic cell lines SW1990, Capan-1 (Cap), AsPC-1 (ASPC), P3, PANC-1 (Pan-1) and MIAPaCa-2 (MIA) were repeatedly given individual dose of X-rays with liner accelerator to induce radiation resistance, the changes of cell morphology, cell cycle and radio sensibility in the induced cell lines were compared with the parental cell lines at the end of inducing course.


RESULTS
Compared with the parental cells, there were significant changes in morphology in the pancreatic cancer cell sublines after the radiation. Cell cycle analysis suggested that SW1990-R, ASPC-R, MIA-R, PAN-R and P3-R had lower G(2)/M and greater SF(2) (survival fraction after 2 Gy irradiation) compared with the parental cell lines.


CONCLUSIONS
The method of radiating cells step by step and repeatedly is viable to establish radio-resistant pancreatic cancer cell lines.
Jiang
Hua
H
Department of General Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.
Zhao
Yu-pei
YP
Chen
Ge
G
Wu
Wei-wei
WW
Zhang
Fu-quan
FQ
Dong
Yu-xing
YX
Wu
Yuan-de
YD
D018929:Cell Culture Techniques / Q000379:methods; D002453:Cell Cycle / Q000528:radiation effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000528:radiation effects*; D048430:Cell Shape / Q000528:radiation effects; D048429:Cell Size / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D010190:Pancreatic Neoplasms / Q000473:pathology / Q000503:physiopathology; D011836:Radiation Tolerance