[The action mechanism of 8-azaadenine and its metal complexes with DNA].
The mechanism of the interactions of 8-azaadenine and its Co2+ and Cu2+ complexes with DNA was studied by UV and fluorescence spectra using ethidium bromide(EB) as the probe molecule. When pH 9. 86, the action of 8-azaadenine-Co2+ with DNA was electrostatic effect and certain kind of intercalation action, when pH 7. 4, the action of 8-azaadenine-Cu2+ with DNA was intercalation action and certain kind of electrostatic effect, when pH 2. 85, the action was intercalation action and certain kind of electrostatic effect. But when pH 7. 4, 8-azaadenine hardly influenced the intercalation action of EB and DNA, and the action of 8-azaadenine and DNA was electrostatic action.
Xi
Xiao-li
XL
Institute of Molecular Science, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China.
Yang
Man-man
MM
Yang
Pin
P
D000225:Adenine / Q000031:analogs & derivatives* / Q000737:chemistry; D003035:Cobalt / Q000737:chemistry; D003300:Copper / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D004996:Ethidium / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D055672:Static Electricity
D003035:Cobalt; D003300:Copper; D004247:DNA; D004996:Ethidium; D000225:Adenine; C006654:8-azaadenine