Simultaneous determination of cefazolin or ceftizoxime in presence of ascorbic acid from pharmaceutical formulation and human serum by RP-HPLC.
A rapid, sensitive and specific RP-HPLC method involving HPLC-UV detection was developed and validated for simultaneous determination and quantification of cefazoline or ceftizoxime in presence of ascorbic acid. Chromatography was carried out on a pre-packed Kromasil 100, C(18) (5 microm 25 x 0.46 cm) column using acetonitrile: water (60:40; v/v) as mobile phase at a flow rate of 0.75 ml/minute and effluent were monitored at 265 nm for cefazoline and ascorbic acid while at 270 nm for ceftizoxime. The assay was linear over the concentration range of 0.05-100 microg/ml. The method is convenient for determination of cefazoline or ceftizoxime in presence of ascorbic acid with percent recovery ranging from 99.0-100.0% with an inter and intra day CV <3%. The method does not require more than 8 minutes for analysis with good peak resolution and low LOD 0.1 microg/ml and LOQ 0.3 microg/ml.
Arayne
M Saeed
MS
Department of Chemistry, University of Karachi, Karachi-75270, Pakistan. arayne@gawab.com
Sultana
Najma
N
Bi Bi
Zakia
Z
D000328:Adult; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000097:blood*; D001205:Ascorbic Acid / Q000032:analysis / Q000097:blood*; D002138:Calibration; D002437:Cefazolin / Q000032:analysis / Q000097:blood*; D015296:Ceftizoxime / Q000032:analysis / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D004304:Dosage Forms / Q000592:standards; D006801:Humans; D016014:Linear Models; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D000900:Anti-Bacterial Agents; D004304:Dosage Forms; D015296:Ceftizoxime; D002437:Cefazolin; D001205:Ascorbic Acid