title: High-resolution whole organ imaging using two-photon tissue cytometry.
abstract: Three-dimensional (3-D) tissue imaging offers substantial benefits to a wide range of biomedical investigations from cardiovascular biology, diabetes, Alzheimer's disease to cancer. Two-photon tissue cytometry is a novel technique based on high-speed multiphoton microscopy coupled with automated histological sectioning, which can quantify tissue morphology and physiology throughout entire organs with subcellular resolution. Furthermore, two-photon tissue cytometry offers all the benefits of fluorescence-based approaches including high specificity and sensitivity and appropriateness for molecular imaging of gene and protein expression. We use two-photon tissue cytometry to image an entire mouse heart at subcellular resolution to quantify the 3-D morphology of cardiac microvasculature and myocyte morphology spanning almost five orders of magnitude in length scales.
authors.affiliation: Massachusetts Institute of Technology, Division of Biological Engineering, Cambridge, Massachusetts 02139, USA. tragan@mit.edu
authors.forename: Timothy
authors.initials: T
authors.lastname: Ragan
authors.forename: Jeremy D
authors.initials: JD
authors.lastname: Sylvan
authors.forename: Ki Hean
authors.initials: KH
authors.lastname: Kim
authors.forename: Hayden
authors.initials: H
authors.lastname: Huang
authors.forename: Karsten
authors.initials: K
authors.lastname: Bahlmann
authors.forename: Richard T
authors.initials: RT
authors.lastname: Lee
authors.forename: Peter T C
authors.initials: PT
authors.lastname: So
mesh_terms: D000818:Animals; D004867:Equipment Design; D019544:Equipment Failure Analysis; D019044:Image Cytometry / Q000295:instrumentation* / Q000379:methods; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000295:instrumentation* / Q000379:methods; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation* / Q000379:methods; D009206:Myocardium / Q000166:cytology*; D032383:Myocytes, Cardiac / Q000648:ultrastructure*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D046509:Tissue Culture Techniques / Q000295:instrumentation / Q000379:methods