Signal enhancement in electronic detection of DNA hybridization.
Electronic detection of the specific recognition between complementary DNA sequences is investigated. DNA probes are immobilized at different lateral positions on a Poly( L -lysine)-coated surface of an integrated silicon transistor array. Hybridization and field effect detection are done with the solid surface immersed in electrolyte solutions. Differential measurements are performed, where DNA hybridization leads to surface potential shifts between the transistors of the array. We experimentally show that these differential signals of hybridization can be enhanced significantly by changing the salt concentration between hybridization and detection.
Gentil
C
C
Laboratoire Pierre Aigrain, Départment de Physique de l'Ecole Normale Supérieure, 24 rue Lhomond, 75005 Paris, France.
Philippin
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G
Bockelmann
U
U
D000327:Adsorption; D000818:Animals; D001703:Biophysics / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D004573:Electrolytes; D004581:Electronics; D004583:Electrons; D005821:Genetic Techniques; D006801:Humans; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D012492:Salts / Q000494:pharmacology; D012815:Signal Processing, Computer-Assisted
D004573:Electrolytes; D012492:Salts; D004247:DNA