In vitro gene transfer using cationic vectors, electroporation and their combination.
BACKGROUND
The aim of this study was to investigate the transfection efficiency of cationic vectors (polyethylenimine; PEI25K and lipofectamine), electroporation and their combination in the human cancer cell lines, Raji human lymphoma and KB human oral carcinoma.


METHODS
Raji human lymphoma and KB human oral carcinoma cell lines were transfected with pcDNA3-CMV-Luc at various N/P ratios of cationic vectors and voltages of electroporation, as well as with a combination of the cationic vectors and electroporation.


RESULTS
The major findings were: (a) cationic vectors or electroporation alone increased transfection efficiency; (b) cationic vectors inhibited the transfection efficiency by electroporation.


CONCLUSIONS
Our results demonstrate that cationic vectors and electroporation are feasible and efficient in transfecting human cancer cell lines. However, a combination of cationic vectors and electroporation is ineffective.
Weecharangsan
Wanlop
W
College of Pharmacy, NCI OSU Comprehensive Cancer Center, NSF Nanoscales Science and Engineering Center (NSEC) for Affordable Nanoengineering of Polymeric Biomedical Devices (CANPBD), The Ohio State University, Columbus, 43210, USA.
Opanasopit
Praneet
P
Lee
Robert J
RJ
D002412:Cations; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics; D053672:Electrochemotherapy / Q000379:methods*; D005822:Genetic Vectors / Q000008:administration & dosage*; D006801:Humans; D007624:KB Cells; D008055:Lipids / Q000008:administration & dosage*; D008223:Lymphoma / Q000235:genetics / Q000628:therapy*; D009369:Neoplasms / Q000235:genetics / Q000628:therapy*; D011094:Polyethyleneimine / Q000008:administration & dosage*; D014162:Transfection / Q000379:methods
D002412:Cations; D008055:Lipids; C086724:Lipofectamine; D011094:Polyethyleneimine; D004247:DNA