title: Effects of reticuloendotheliosis virus and Marek's disease virus infection and co-infection on IFN-gamma production in SPF chickens.
abstract: Two experiments were used to examine the potential role of IFN-gamma in chickens infected with reticuloendotheliosis virus (REV) and Marek's disease virus (MDV). First, chickens were infected with REV and/or MDV at 5 days of age and examined from 3 to 50 days post-infection (dpi). In REV+MDV co-infection chickens, IFN-gamma ELISA demonstrated a 3-fold increase at 7 dpi compared to the controls, while REV alone caused a 5-fold increase, the IFN-gamma levels peaked, and then gradually decreased. IFN-gamma levels significantly decreased in MDV infection at 3 dpi and 15 dpi. Second, experiments were designed to determine the effects of different viruses and ConA on IFN-gamma production. For REV- or MDV-infected chickens, the IFN-gamma levels decreased slightly after adding ConA. This is the first report of IFN-gamma production in SPF chickens infected with REV and MDV measured by directly quantitative method.
authors.affiliation: College of Veterinary Medicine, Shangdong Agricultural University, Taian, Shandong, China.
authors.forename: Yu-shu
authors.initials: YS
authors.lastname: Zheng
authors.forename: Zhi-zhong
authors.initials: ZZ
authors.lastname: Cui
authors.forename: Pu
authors.initials: P
authors.lastname: Zhao
authors.forename: Hong-mei
authors.initials: HM
authors.lastname: Li
authors.forename: Cui-yan
authors.initials: CY
authors.lastname: Liu
authors.forename: Zhao-ju
authors.initials: ZJ
authors.lastname: Tian
authors.forename: Hong-kun
authors.initials: HK
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology / Q000821:virology; D025922:Mardivirus / Q000276:immunology*; D008380:Marek Disease / Q000150:complications* / Q000276:immunology / Q000821:virology*; D011897:Random Allocation; D017986:Reticuloendotheliosis Viruses, Avian / Q000276:immunology*; D012192:Retroviridae Infections / Q000150:complications / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D013047:Specific Pathogen-Free Organisms; D014412:Tumor Virus Infections / Q000150:complications / Q000276:immunology / Q000662:veterinary* / Q000821:virology
chemical_list: D007371:Interferon-gamma