title: CD133, one of the markers of cancer stem cells in Hep-2 cell line.
abstract: OBJECTIVE
  In recent years, a growing body of evidence has been reported that a tumor clone is organized as a hierarchy that originates from rare stem cells. CD133, a cell surface antigen, was identified as a stem cell maker for human leukemia, brain tumors, and prostate cancer. The purpose of this study was to detect the expression of CD133, a putative marker of cancer stem cells in the Hep-2 cell line, and isolate CD133 positive cells to observe their proliferation and differentiation ability in vitro.
  
  
  METHODS
  Immunocytochemical staining technology and flow cytometry were used to detect the expression of the putative stem cell marker CD133 in a Hep-2 cell line. The immunomagnetic beads were applied to purify CD133 positive cells. CD133+ tumor cells were cultured in vitro to observe their ability to proliferate and differentiate.
  
  
  RESULTS
  Only a small proportion (<5%) of cells in the Hep-2 cell line expressed CD133. CD133+ cells possess a marked capacity for self renewal, extensive proliferation, and mutilineal differentiation potency in vitro.
  
  
  CONCLUSIONS
  CD133 is one of the markers for cancer stem cells in human laryngeal tumors, the Hep-2 cell line.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Fudan University, Affiliated Eye, Ear, Nose and Throat Hospital, Shanghai, China. zhlwc@online.sh.cn
authors.forename: Liang
authors.initials: L
authors.lastname: Zhou
authors.forename: Xudong
authors.initials: X
authors.lastname: Wei
authors.forename: Lei
authors.initials: L
authors.lastname: Cheng
authors.forename: Jie
authors.initials: J
authors.lastname: Tian
authors.forename: Jack J
authors.initials: JJ
authors.lastname: Jiang
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD / Q000096:biosynthesis*; D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005434:Flow Cytometry; D006023:Glycoproteins / Q000096:biosynthesis*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism / Q000473:pathology*; D008856:Microscopy, Fluorescence; D014411:Neoplastic Stem Cells / Q000378:metabolism* / Q000473:pathology; D010455:Peptides
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D014408:Biomarkers, Tumor; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides