title: Maternal-neonatal 8-hydroxy-deoxyguanosine serum concentrations as an index of DNA oxidation in association with the mode of labour and delivery.
abstract: OBJECTIVE
  To investigate the effect of the mode of labour and delivery on the total antioxidant status (TAS), and the biomarker of DNA oxidation, 8-hydroxy-deoxyguanosine (8-OHdG) serum levels, in mothers and their newborns.
  
  
  METHODS
  Some 106 women with normal pregnancy and normal blood biochemical parameters were divided into 4 groups: Group A (n=28) with normal labour and vaginal delivery (VG), Group B (n=25) with scheduled cesarean section (CS), Group C (n=26) with 'emergency' CS, and Group D (n=27) with prolonged labour+VG. Blood was obtained from the mothers at the beginning of labour, and immediately after delivery (pre- and post-delivery), as well as from the umbilical cord (CB). TAS, 8-OHdG and creatine kinase (CK) were measured in the sera with appropriate methodology.
  
  
  RESULTS
  TAS levels were almost similar in all the groups pre-delivery, and in CB irrespective of the mode of labour and delivery, and remarkably decreased in Groups C and D post-delivery. 8-OHdG levels in Group C (0.94+/-0.08 ng/ml) and Group D (0.98+/-0.08 ng/ml) were significantly higher than those in Group A (0.26+/-0.01 ng/ml, p<0.001) and Group B (0.28+/-0.07 ng/ml, p<0.001) post-delivery. 8-OHdG levels were low in CB, independent of the mode of labour. CK positively correlated with 8-OHdG (r=0.48, p<0.001), the latter negatively correlated with TAS (r=-0.53, p<0.01).
  
  
  CONCLUSIONS
  The lowest TAS and the highest 8-OHdG levels were found in Groups C and D post-delivery, probably due to the long-term participation of the mothers' skeletal and uterus muscles, whereas 8-OHdG levels were low in CB irrespective of the mode of delivery, possibly as a consequence of the antioxidant action of the placenta and/or the low lipid levels in the serum of the umbilical cord.
authors.affiliation: Institute of Child Health, Research Center, Department of Clinical Biochemistry Aghia Sophia Children's Hospital, and First Department of Obstetrics and Gynecology, Athens University Medical School, Greece.
authors.forename: Kleopatra H
authors.initials: KH
authors.lastname: Schulpis
authors.forename: Christina
authors.initials: C
authors.lastname: Lazaropoulou
authors.forename: George D
authors.initials: GD
authors.lastname: Vlachos
authors.forename: George A
authors.initials: GA
authors.lastname: Partsinevelos
authors.forename: Kelly
authors.initials: K
authors.lastname: Michalakakou
authors.forename: Stavroula
authors.initials: S
authors.lastname: Gavrili
authors.forename: Antonia
authors.initials: A
authors.lastname: Gounaris
authors.forename: Aris
authors.initials: A
authors.lastname: Antsaklis
authors.forename: Ioannis
authors.initials: I
authors.lastname: Papassotiriou
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000975:Antioxidants / Q000032:analysis; D015415:Biomarkers / Q000097:blood; D002585:Cesarean Section; D003402:Creatine Kinase / Q000097:blood; D036861:Delivery, Obstetric; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000097:blood; D004630:Emergencies; D005260:Female; D005312:Fetal Blood / Q000737:chemistry*; D006801:Humans; D007231:Infant, Newborn; D007743:Labor, Obstetric / Q000097:blood*; D007744:Obstetric Labor Complications / Q000097:blood; D018384:Oxidative Stress / Q000502:physiology*; D049590:Postpartum Period; D011247:Pregnancy
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003402:Creatine Kinase; D003849:Deoxyguanosine