Cytoprotective effect of Podophyllum hexandrum against gamma radiation is mediated via hemopoietic system stimulation and up-regulation of heme-oxygenase-1 and the prosurvival multidomain protein Bcl-2.
The radioprotective effect of a hydroalcoholic extracted material (REC-2000) from the rhizome of Podophyllum hexandrum was studied in mice exposed to lethal gamma radiation (10 Gy). The extract (REC-2000) was found to restore the hemoglobin content (14.73 +/- 0.33) and total leukocyte count (TLC) (4166.66 +/- 0.02) in lethally (10 Gy) gamma-irradiated mice on the 15th day in comparison to the radiation control mice. The hemoglobin content of the drug + radiation group was observed to be significantly (21.25%) higher than the radiation control group on the 10th day. Similarly, the TLC was significantly increased (83.33 times) in the drug + radiation group as compared to a radiation (10 Gy) only group on the 10th day. Enhanced expression of heme-oxygenase-1 and Bcl-2 protein observed by Western blotting further supports the observation of hemopoietic recovery in irradiated mice. These findings indicate that the bioactive constituents present in REC-2000 exert the radioprotective effect by modulating the hemopoietic system.
Rajesh
Arora
A
Medicinal and Aromatic Plants Laboratory, Division of Radiation Biology and Radioprotection, Institute of Nuclear Medicine and Allied Sciences, Brig. SK Mazumdar Road, Delhi-110 054, India. rajesharoradr@rediffmail.com
Sagar
R
R
Singh
S
S
Kumar
R
R
Sharma
A K
AK
Prasad
J
J
Singh
S
S
Gupta
M
M
Sharma
R K
RK
Puri
S C
SC
Krishna
B
B
Siddiqui
M S
MS
Lahiri
S S
SS
Tripathi
R P
RP
Qazi
G N
GN
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005720:Gamma Rays / Q000009:adverse effects*; D006413:Hematopoietic System; D051547:Heme Oxygenase-1 / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism; D007962:Leukocytes; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010936:Plant Extracts / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D011035:Podophyllum / Q000737:chemistry*; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2; D011833:Radiation Injuries, Experimental; D011837:Radiation-Protective Agents / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D015854:Up-Regulation
D006454:Hemoglobins; D010936:Plant Extracts; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D011837:Radiation-Protective Agents; C052617:Bcl2 protein, mouse; D051547:Heme Oxygenase-1