title: Ostreolysin affects rat aorta ring tension and endothelial cell viability in vitro.
abstract: The effects of ostreolysin, a cardiotoxic cytolysin produced by the edible oyster mushroom (Pleurotus ostreatus), were studied on tension development in isolated rat aortic ring. Its cytotoxic effects on human umbilical vein endothelial cells and Chinese hamster lung fibroblasts were also studied. Ostreolysin induced a concentration-dependent increase in aortic ring tension in the range from 5 to 30 microg/ml, and was cytotoxic to both cell lines. These effects could contribute significantly to its previously observed cardiotoxicity.
authors.affiliation: Department of Biology, Biotechnical Faculty, University of Ljubljana, Vecna pot 111, 1000 Ljubljana, Slovenia.
authors.forename: Katja
authors.initials: K
authors.lastname: Rebolj
authors.forename: Urska
authors.initials: U
authors.lastname: Batista
authors.forename: Kristina
authors.initials: K
authors.lastname: Sepcić
authors.forename: Vojteh
authors.initials: V
authors.lastname: Cestnik
authors.forename: Peter
authors.initials: P
authors.lastname: Macek
authors.forename: Robert
authors.initials: R
authors.lastname: Frangez
mesh_terms: D000818:Animals; D001011:Aorta / Q000187:drug effects* / Q000502:physiology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D006224:Cricetinae; D003412:Cricetulus; D042783:Endothelial Cells / Q000187:drug effects*; D005656:Fungal Proteins / Q000032:analysis / Q000633:toxicity; D006460:Hemolysin Proteins / Q000032:analysis / Q000633:toxicity*; D006801:Humans; D009129:Muscle Tonus / Q000187:drug effects*; D020076:Pleurotus / Q000737:chemistry*; D051381:Rats; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D005656:Fungal Proteins; D006460:Hemolysin Proteins; D013778:Tetrazolium Salts; D013844:Thiazoles; C461280:ostreolysin; C022616:thiazolyl blue