Viral MRI contrast agents: coordination of Gd by native virions and attachment of Gd complexes by azide-alkyne cycloaddition.
Icosahedral virus particles decorated with a Gd(DOTA) analogue by Cu-mediated azide-alkyne cycloaddition (CuAAC) and/or with Gd(3+) ions by coordination to the viral nucleoprotein show increased T(1) relaxivity relative to free Gd(DOTA) complexes in solution.
Prasuhn
Duane E
DE
Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA, USA.
Yeh
Robert M
RM
Obenaus
Andre
A
Manchester
Marianne
M
Finn
M G
MG
D000480:Alkynes / Q000737:chemistry*; D001386:Azides / Q000737:chemistry*; D017798:Comovirus / Q000737:chemistry; D003287:Contrast Media; D018360:Crystallography, X-Ray; D003500:Cyclization; D005682:Gadolinium / Q000737:chemistry*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D014771:Virion / Q000737:chemistry*
D000480:Alkynes; D001386:Azides; D003287:Contrast Media; D005682:Gadolinium