title: The localization of ribosomal DNA in Sciaridae (Diptera: Nematocera) reassessed.
abstract: The chromosomal localization of ribosomal DNA (rDNA) was studied in polytene and diploid tissues of four sciarid species, Trichosia pubescens, Rhynchosciara americana, R. milleri and Schwenkfeldina sp. While hybridization to mitotic chromosomes showed the existence of a single rDNA locus, ribosomal probes hybridized to more than one polytene chromosome region in all the species analyzed as a result of micronucleolar attachment to specific chromosome sites. Micronucleoli are small, round bodies containing transcriptionally active, probably extrachromosomal rDNA. In T. pubescens the rDNA is predominantly localized in chromosome sections X-10 and X-8. In R. americana the rDNA is frequently found associated with centromeric heterochromatin of the chromosomes X, C, B and A, and also with sections X-1 and B-13. Ribosomal probes in R. milleri hybridized with high frequency to pericentric and telomeric regions of its polytene complement. Schwfenkfeldina sp. displays a remarkably unusual distribution of rDNA in polytene nuclei, characterized by the attachment of micronucleoli to many chromosome regions. The results showed that micronucleoli preferentially associate with intercalary or terminal heterochromatin of all sciarid flies analyzed and, depending on the species, are attached to a few (Trichosia), moderate (Rhynchosciara) or a large (Schwenkfeldina sp.) number of polytene chromosome sites.
authors.affiliation: Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo, Rua do Matão 277, Cidade Universitária, São Paulo, SP, Brazil.
authors.forename: Christiane Rodriguez Gutierrez
authors.initials: CR
authors.lastname: Madalena
authors.forename: José Mariano
authors.initials: JM
authors.lastname: Amabis
authors.forename: Ann Jacob
authors.initials: AJ
authors.lastname: Stocker
authors.forename: Eduardo
authors.initials: E
authors.lastname: Gorab
mesh_terms: D000818:Animals; D002466:Cell Nucleolus / Q000235:genetics / Q000378:metabolism; D002875:Chromosomes / Q000235:genetics / Q000378:metabolism; D004275:DNA, Ribosomal / Q000235:genetics* / Q000378:metabolism*; D004175:Diptera / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D015347:RNA Probes
chemical_list: D004275:DNA, Ribosomal; D015347:RNA Probes