[Study of interaction of ciprofloxacin probe and calf thymus DNA using fluorescence spectrometry].
The interaction of ciprofloxacin and calf thymus DNA (ctDNA) was studied using UV spectrometry and fluorescence spectrometry. Ciprofloxacin exhibited a fluorescence excitation at 270 nm and a fluorescence emission at 420 nm in pH 7.0 phosphate buffer solutions due to the groove binding of ciprofloxacin on calf thymus ctDNA. The binding constant was 2. 64 X 104 mol(-1) x L(25 degrees C). Based on the fluorescence quenching, a novel method for sensitive determination of calf thymus ctDNA concentration ranging from 80 nmol x L(-1) to 45 micromol x L(-1) was developed. The relative standard deviation for eleven detections of 25 micromol x L(-1) ctDNA was 4. 2%.
Gan
Ning
N
College of Material Science and Chemical Engineering, Department of Chemistry, Ningbo University, Ningbo, China.
Ge
Cong-xin
CX
D002939:Ciprofloxacin / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D015394:Molecular Structure; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D002939:Ciprofloxacin; D004247:DNA; C427203:calf thymus DNA