[Modification of vascular endothelial cell treatment for patch clamp study].
A modified protocol for quick and economic treatment of cultured human umbilical vein endothelial cells has been established, which result in high viability of the cells to allow better performance in patch-clamp studies. The electrophysiological properties of Ca (2+)-activated K(+) (KCa) channel of the cultured cells were investigated with a cell-attached configuration. With this modified treatment method, the cultured cells appear fusiform in shape under microscope, and KCa channel currents could be detected readily, suggesting their eligibility for patch-clamp studies.
Zhang
Zhi-Huan
ZH
Department of Respiratory Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. zhzhuan@fimmu.com
Cai
Shao-Xi
SX
Cai
Chun-Qing
CQ
Zhao
Hai-Jin
HJ
D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology*; D006801:Humans; D008564:Membrane Potentials / Q000502:physiology; D018408:Patch-Clamp Techniques / Q000379:methods; D024681:Potassium Channels, Calcium-Activated / Q000502:physiology*; D015203:Reproducibility of Results
D024681:Potassium Channels, Calcium-Activated