title: [Epidemiological survey of pneumococcus serotypes in pediatric patients with acute suppurative otitis media].
abstract: To determine the distribution of Streptococcus pneumoniae serotypes isolated from patients under 6 years of age with acute suppurative otitis media, to calculate the serotype coverage of 7-valent pneumococcal conjugate vaccine, and to clarify trends in PCG-resistant Streptococcus pneumoniae, we conducted a one-year prospective study from April 2005 to March 2006 at 10 medical institutions in Hokkaido, Miyagi, Chiba, Tokyo, Kanagawa, and Mie, Japan. Specimens collected by tympanotomy or myringotomy numbered 856, and 691 strains were isolated from 599 specimens. Of these, 219 isolates (31.7%) were identified as Streptococcus pneumoniae and 201 met study requirements. The most common serotype was 19F (52 isolates, 25.9%), followed by 6B (30 isolates, 14.9%) and 23F (24 isolates, 11.9%). Seven-valent vaccine serotype coverage was 62.7%. The percentage of PSSP was 40.3%, PISP 42.8%, and PRSP 16.9%, resistant strains (PISP and PRSP) combined accounted for 59.7%. Seven-valent vaccine serotype coverage for PISP was 80.2% and PRSP 82.4%. PBP gene mutation was observed in 175 isolates (87.1%), including 70 of gPISP (34.8%) and 105 of gPRSP (52.2%). Gene mutation induced by macrolides was found in 176 isolates (87.6%).
authors.affiliation: National Hospital Organization Mie Hospital.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Kamiya
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Kato
authors.forename: Takehiro
authors.initials: T
authors.lastname: Togashi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Iwata
authors.forename: Tomomichi
authors.initials: T
authors.lastname: Kurosaki
authors.forename: Shunkichi
authors.initials: S
authors.lastname: Baba
authors.forename: Sawako
authors.initials: S
authors.lastname: Masuda
authors.forename: Shigeki
authors.initials: S
authors.lastname: Sato
authors.forename: Osamu
authors.initials: O
authors.lastname: Yoshimura
authors.forename: Masato
authors.initials: M
authors.lastname: Fujii
authors.forename: Akiko
authors.initials: A
authors.lastname: Shimada
authors.forename: Katsunori
authors.initials: K
authors.lastname: Yagi
authors.forename: Hisakazu
authors.initials: H
authors.lastname: Yano
authors.forename: Rinya
authors.initials: R
authors.lastname: Sugita
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Fujimaki
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Komatsu
authors.forename: Toshiro
authors.initials: T
authors.lastname: Tango
mesh_terms: D000208:Acute Disease; D002675:Child, Preschool; D024881:Drug Resistance, Bacterial; D005260:Female; D006801:Humans; D007223:Infant; D018942:Macrolides / Q000494:pharmacology; D008297:Male; D010035:Otitis Media, Suppurative / Q000382:microbiology*; D010403:Penicillin Resistance; D046915:Penicillin-Binding Proteins / Q000235:genetics; D011018:Pneumonia, Pneumococcal / Q000453:epidemiology*; D011446:Prospective Studies; D012703:Serotyping; D013296:Streptococcus pneumoniae / Q000145:classification* / Q000187:drug effects / Q000302:isolation & purification
chemical_list: D018942:Macrolides; D046915:Penicillin-Binding Proteins