Patterns of host genome-wide gene transcript abundance in the peripheral blood of patients with acute dengue hemorrhagic fever.
Responses by peripheral blood leukocytes may contribute to the pathogenesis of dengue hemorrhagic fever (DHF). We used DNA microarrays to reveal transcriptional patterns in the blood of 14 adults with DHF. Acute DHF was defined by an abundance of transcripts from cell cycle- and endoplasmic reticulum (ER)-related genes, suggesting a proliferative response accompanied by ER stress. Transcript-abundance levels for immunoresponse-associated genes, including cell surface markers, immunoglobulin, and innate response elements, were also elevated. Twenty-four genes were identified for which transcript abundance distinguished patients with dengue shock syndrome (DSS) from those without DSS. All the gene transcripts associated with DSS, many of which are induced by type I interferons, were less abundant in patients with DSS than in those without DSS. To our knowledge, these data provide the first snapshot of gene-expression patterns in peripheral blood during acute dengue and suggest that DSS is associated with attenuation of selected aspects of the innate host response.
Simmons
Cameron P
CP
Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam. camsimmons1@gmail.com
Popper
Stephen
S
Dolocek
Christiane
C
Chau
Tran Nguyen Bich
TN
Griffiths
Michael
M
Dung
Nguyen Thi Phuong
NT
Long
Truong Hoang
TH
Hoang
Dang Minh
DM
Chau
Nguyen Vinh
NV
Thao
Le Thi Thu
le TT
Hien
Tran Tinh
TT
Relman
David A
DA
Farrar
Jeremy
J
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D001402:B-Lymphocytes / Q000276:immunology* / Q000821:virology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002648:Child; D003289:Convalescence; D016207:Cytokines / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry; D003716:Dengue Virus / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D018816:Genes, cdc / Q000502:physiology; D006801:Humans; D008297:Male; D005810:Multigene Family / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012333:RNA, Messenger / Q000097:blood; D012367:RNA, Viral / Q000097:blood; D019595:Severe Dengue / Q000276:immunology / Q000821:virology*; D014158:Transcription, Genetic; D014744:Vietnam
D016207:Cytokines; D017931:DNA Primers; D012333:RNA, Messenger; D012367:RNA, Viral