NF-Y and Sp1 are involved in transcriptional regulation of rat SND p102 gene.
SND p102 is a rat liver endoplasmic reticulum cholesterol ester hydrolase recently described as a member of a conserved family of transcriptional coactivators that promotes phospholipid secretion into lipoproteins when overexpressed in hepatocytes. In this work, we report first evidence for a mechanism of transcriptional regulation for the SND p102 (Snd1) gene. Promoter activity of 5' deletion fragments determined in human HepG2 and rat McA-RH7777 hepatoma cells by luciferase reporter gene assays showed a minimal promoter involving two inverted CCAAT boxes. EMSA demonstrated specific binding of Sp1 to GC boxes in the proximal, highly active promoter region besides that of NF-Y to CCAAT boxes reported earlier. Site-directed disruption of such CCAAT and GC boxes led to reduction in transcriptional activity, confirming the functional implication of NF-Y and Sp1 in SND p102 gene transcription.
Rodríguez
Lorena
L
Department of Physiology, University of the Basque Country Medical School, Barrio Sarriena s/n, 48940 Leioa, Vizcaya, Spain.
Ochoa
Begoña
B
Martínez
María José
MJ
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D023081:CCAAT-Binding Factor / Q000378:metabolism*; D045744:Cell Line, Tumor; D018899:CpG Islands; D024202:Electrophoretic Mobility Shift Assay; D004720:Endonucleases; D005786:Gene Expression Regulation; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics*; D009841:Oligonucleotides / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D016329:Sp1 Transcription Factor / Q000378:metabolism*; D014158:Transcription, Genetic; D014162:Transfection
D023081:CCAAT-Binding Factor; D009687:Nuclear Proteins; D009841:Oligonucleotides; D011993:Recombinant Fusion Proteins; D016329:Sp1 Transcription Factor; D008156:Luciferases; D004720:Endonucleases; C490639:Snd1 protein, rat