The core component of the mammalian SWI/SNF complex SMARCD3/BAF60c is a coactivator for the nuclear retinoic acid receptor.
Retinoic acid receptors (RARs) activate transcription by recruiting coactivator complexes such as histone acetyltransferases (HAT) and the mediator complex, to increase chromatin accessibility by general transcription factors and to promote transcription initiation. Indirect evidences have suggested a role for the ATP-dependent chromatin remodeling complex SWI/SNF in RAR-mediated transcription. Here we demonstrate that two highly related subunits of the core SWI/SNF complex, BAF60c1 and BAF60c2, interact physically with retinoid receptors and are coactivators for RARs. This coactivating property is dependent on SRC1 expression, showing that HATs and SWI/SNF cooperate in this retinoid-controlled transcriptional process.
Flajollet
Sébastien
S
Institut Pasteur de Lille, Département d'Athérosclérose, Lille F-59019, France.
Lefebvre
Bruno
B
Cudejko
Céline
C
Staels
Bart
B
Lefebvre
Philippe
P
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D004268:DNA-Binding Proteins; D006367:HeLa Cells; D051548:Histone Acetyltransferases / Q000378:metabolism; D006801:Humans; D051379:Mice; D009124:Muscle Proteins / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D056920:Nuclear Receptor Coactivator 1; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding / Q000187:drug effects; D021122:Protein Subunits / Q000378:metabolism; D016601:RNA-Binding Proteins; D018168:Receptors, Retinoic Acid / Q000378:metabolism*; D000072482:Retinoic Acid Receptor alpha; D012176:Retinoids / Q000494:pharmacology; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
D002352:Carrier Proteins; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D009124:Muscle Proteins; C110421:Mybbp1a protein, mouse; D009479:Neuropeptides; D009687:Nuclear Proteins; D021122:Protein Subunits; C000607071:RARA protein, human; D016601:RNA-Binding Proteins; C000607072:Rara protein, mouse; D018168:Receptors, Retinoic Acid; D000072482:Retinoic Acid Receptor alpha; D012176:Retinoids; C494055:Smarcd3 protein, mouse; C465207:Smarce1 protein, mouse; D014157:Transcription Factors; C073964:AF2 neuropeptide; D051548:Histone Acetyltransferases; C538859:NCOA1 protein, human; C538861:Ncoa1 protein, mouse; D056920:Nuclear Receptor Coactivator 1