A simple method for measuring long-range 1H-13C coupling constants in organic molecules.
This study presents a simple method for measuring long-range heteronuclear coupling constants between protons and proton-bearing carbons. The approach involves recording two conventional 1D-TOCSY experiments in which the offset of the selective proton pulse is set on the low- and high-frequency 13C satellites of an isolated proton signal, Hi. Long-range heteronuclear coupling values between the 13Ci bonded to Hi and the protons Hj,k...n that belong to the same spin system were easily determined from the relative displacement of the relayed Hj,k...n signals in the satellite-selective TOCSY spectra. The sense of the displacement indicated the sign of the coupling constants.
Vidal
Paloma
P
Discovery Chemistry Research & Technologies, Lilly Research Laboratories, Centro de Investigación Lilly, Avenida de la Industria 30, 28108 Alcobendas, Madrid, Spain.
Esturau
Nuria
N
Parella
Teodor
T
Espinosa
Juan F
JF
D002244:Carbon / Q000737:chemistry; D006859:Hydrogen / Q000737:chemistry; D007532:Isoleucine / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008610:Menthol / Q000737:chemistry; D009930:Organic Chemicals / Q000737:chemistry*; D011522:Protons; D013267:Stilbenes / Q000737:chemistry
D009930:Organic Chemicals; D011522:Protons; D013267:Stilbenes; D007532:Isoleucine; D008610:Menthol; D002244:Carbon; D006859:Hydrogen; C025906:stilbene oxide