Role of peroxynitrite on cytoskeleton alterations and apoptosis in renal ischemia-reperfusion.
During renal ischemia-reperfusion (I/R) injury, apoptosis has been reported as a very important contributor to final kidney damage. The determinant role of cytoskeleton derangement in the development of apoptosis has been previously reported, but a clear description of the different mechanisms involved in this process has not been yet provided. The aim of our study was to know the role of peroxynitrite as an inductor of cytoskeleton derangement and apoptosis during renal I/R. Based on a rat kidney I/R model, using experiments in which both the actin cytoskeleton and peroxynitrite generation were pharmacologically manipulated, results indicate that the peroxynitrite produced during the I/R-derived oxidative stress state is able to provoke cytoskeleton derangement and apoptosis development. Thus control of peroxynitrite generation during I/R could be an effective tool for the improvement of cytoskeleton damage and reduction of apoptosis incidence in renal I/R injury.
Viñas
Jose Luis
JL
Department of Experimental Pathology, Instituto de Investigaciones Biomédicas, Institut d'Investigacions Biomediques de Barcelona of the Council for Scientific Research, Barcelona, Spain. jvmbam@iibb.csic.es
Hotter
Gina
G
Pi
Felip
F
Palacios
Luis
L
Sola
Anna
A
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001794:Blood Pressure / Q000502:physiology; D001806:Blood Urea Nitrogen; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D003599:Cytoskeleton / Q000473:pathology*; D020287:In Situ Nick-End Labeling; D007674:Kidney Diseases / Q000473:pathology*; D007677:Kidney Function Tests; D008297:Male; D018613:Microscopy, Confocal; D030421:Peroxynitrous Acid / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology*; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
D030421:Peroxynitrous Acid; C002744:3-nitrotyrosine; D014443:Tyrosine; D053148:Caspase 3