title: Severe acute respiratory syndrome coronavirus accessory protein 6 is a virion-associated protein and is released from 6 protein-expressing cells.
abstract: Analysis of severe acute respiratory syndrome coronavirus (SCoV) by either sucrose gradient equilibrium centrifugation or a virus capture assay using an anti-SCoV S protein antibody demonstrated that the SCoV 6 protein, which is one of the accessory proteins of SCoV, was incorporated into virus particles. Coexpression of the SCoV S, M, E, and 6 proteins was sufficient for incorporation of the 6 protein into virus-like particles. Cells transfected with plasmid expressing the 6 protein released SCoV 6 protein; however, infected cells released SCoV 6 protein only in association with SCoV particles.
authors.affiliation: Department of Microbiology and Immunology, The University of Texas Medical Branch at Galveston, Galveston, TX 77555-1019, USA.
authors.forename: Cheng
authors.initials: C
authors.lastname: Huang
authors.forename: C J
authors.initials: CJ
authors.lastname: Peters
authors.forename: Shinji
authors.initials: S
authors.lastname: Makino
mesh_terms: D000818:Animals; D002499:Centrifugation, Density Gradient; D002522:Chlorocebus aethiops; D045473:Severe acute respiratory syndrome-related coronavirus / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D014709:Vero Cells; D014764:Viral Proteins / Q000032:analysis / Q000378:metabolism*; D014771:Virion / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D022701:Virosomes / Q000737:chemistry
chemical_list: D014764:Viral Proteins; D022701:Virosomes; C507223:protein 6, SARS virus