title: Arvanil and anandamide up-regulate CD36 expression in human peripheral blood mononuclear cells.
abstract: In this study we analysed the regulation of gene expression by arvanil and anandamide in human peripheral blood mononuclear cells (PBMCs) to clarify their immunosuppressive properties. PBMCs were activated, leading to CD36 down regulation, that was normalized by arvanil and anandamide. We used microarray technology to identify a regulatory pattern associated with cell proliferation in the presence of both substances. CD3-CD28 stimulated PBMCs showed a pattern of up-regulated and down-regulated genes after treatment with these substances. We selected and analysed several genes chosen by their function in the regulation of cell proliferation. We showed a transcriptional control of the CD36 gene by arvanil and anandamide associated with an increased protein expression, thus suggesting a possible role of CD36 in anandamide and arvanil anti-inflammatory pattern.
authors.affiliation: Dipartimento di Scienze Farmaceutiche, Università di Salerno, Fisciano (SA), Italy.
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Malfitano
authors.forename: Gokce Altay
authors.initials: GA
authors.lastname: Toruner
authors.forename: Patrizia
authors.initials: P
authors.lastname: Gazzerro
authors.forename: Chiara
authors.initials: C
authors.lastname: Laezza
authors.forename: Seema
authors.initials: S
authors.lastname: Husain
authors.forename: Davide
authors.initials: D
authors.lastname: Eletto
authors.forename: Pierangelo
authors.initials: P
authors.lastname: Orlando
authors.forename: Luciano
authors.initials: L
authors.lastname: De Petrocellis
authors.forename: Alexandra
authors.initials: A
authors.lastname: Terskiy
authors.forename: Marvin
authors.initials: M
authors.lastname: Schwalb
authors.forename: Emilia
authors.initials: E
authors.lastname: Vitale
authors.forename: Maurizio
authors.initials: M
authors.lastname: Bifulco
mesh_terms: D001095:Arachidonic Acids / Q000276:immunology / Q000494:pharmacology*; D018106:CD28 Antigens / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology; D018955:CD36 Antigens / Q000096:biosynthesis* / Q000097:blood / Q000276:immunology; D002211:Capsaicin / Q000031:analogs & derivatives* / Q000276:immunology / Q000494:pharmacology; D063388:Endocannabinoids; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D053284:Polyunsaturated Alkamides / Q000276:immunology / Q000494:pharmacology*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D001095:Arachidonic Acids; D018106:CD28 Antigens; D017252:CD3 Complex; D018955:CD36 Antigens; D063388:Endocannabinoids; D053284:Polyunsaturated Alkamides; C121153:arvanil; D002211:Capsaicin; C078814:anandamide