[The plasma levels and diagnostic utility of granulocyte colony stimulating factor (G-CSF) and macrophage - colony stimulating factor (M-CSF) in ovarian cancer patients].
G-CSF and M-CSF are of the glycoproteins called colony-stimulating factors (CSFs). Some clinical investigations have shown an autologous production of G-CSF and M-CSF in various human cell lines in vitro and by tumors in vivo.


OBJECTIVE
We have investigated the plasma levels and diagnostic utility of G-CSF M-CSF and commonly accepted tumor marker, such as CA 125 in ovarian cancer patients before surgery and healthy subjects.


METHODS
We tested 30 patients with ovarian cancer and 30 healthy women (control group). G-CSF and M-CSF were determined using enzyme-linked immunosorbent assay (ELISA), CA 125 was measured by microparticle enzyme immunoassay (MEIA).


RESULTS
G-CSF, M-CSF and CA 125 plasma levels were significantly higher in ovarian cancer patients compared to the control group. The diagnostic sensitivity of M-CSF was higher than for G-CSF and CA 125 (57%, 50% i 53%, respectively). The diagnostic specificity was high and equal for all tested parameters (93%). Positive predictive value was higher for M-CSF and CA 125 (equal 89%). Negative predictive value was the highest for M-CSF in comparison to G-C.SF and CA 125 (68%, 65%, 66,7%, respectively).


CONCLUSIONS
Our study suggests that especially M-CSF can be clinically useful in diagnostic ovarian cancer, but further investigation and confirmation by a prospective study is necessary.
ławicki
Sławomir
S
Department of Biochemical Diagnostics, Medical University of Białystok, Poland.
Czygier
Małgorzata
M
Gacuta-Szumarska
Ewa
E
Knapp
Paweł
P
Szmitkowski
Maciej
M
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood*; D018394:CA-125 Antigen / Q000097:blood; D003536:Cystadenocarcinoma / Q000097:blood* / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000097:blood*; D006801:Humans; D016173:Macrophage Colony-Stimulating Factor / Q000097:blood*; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000097:blood* / Q000175:diagnosis*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
D014408:Biomarkers, Tumor; D018394:CA-125 Antigen; D016179:Granulocyte Colony-Stimulating Factor; D016173:Macrophage Colony-Stimulating Factor