title: Hyperbaric oxygen stimulates epidermal reconstruction in human skin equivalents.
abstract: The crucial role of oxygen during the complex process of wound healing has been extensively described. In chronic or nonhealing wounds, much evidence has been reported indicating that a lack of oxygen is a major contributing factor. Although still controversial, the therapeutic application of hyperbaric oxygen (HBO) therapy can aid the healing of chronic wounds. However, how HBO affects reepithelization, involving processes such as keratinocyte proliferation and differentiation, remains unclear. We therefore used a three-dimensional human skin-equivalent (HSE) model to investigate the effects of daily 90-minute HBO treatments on the reconstruction of an epidermis. Epidermal markers of proliferation, differentiation, and basement membrane components associated with a developing epidermis, including p63, collagen type IV, and cytokeratins 6, 10, and 14, were evaluated. Morphometric analysis of hematoxylin and eosin-stained cross sections revealed that HBO treatments significantly accelerated cornification of the stratum corneum compared with controls. Protein expression as determined by immunohistochemical analysis confirmed the accelerated epidermal maturation. In addition, early keratinocyte migration was enhanced by HBO. Thus, HBO treatments stimulate epidermal reconstruction in an HSE. These results further support the importance of oxygen during the process of wound healing and the potential role of HBO therapy in cutaneous wound healing.
authors.affiliation: Tissue Repair and Regeneration Program, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia.
authors.forename: Evette
authors.initials: E
authors.lastname: Kairuz
authors.forename: Zee
authors.initials: Z
authors.lastname: Upton
authors.forename: Rebecca A
authors.initials: RA
authors.lastname: Dawson
authors.forename: Jos
authors.initials: J
authors.lastname: Malda
mesh_terms: D000328:Adult; D002454:Cell Differentiation; D049109:Cell Proliferation; D024141:Collagen Type IV / Q000378:metabolism; D004817:Epidermis / Q000502:physiology*; D006801:Humans; D006931:Hyperbaric Oxygenation; D007150:Immunohistochemistry; D053547:Keratin-14 / Q000378:metabolism; D015603:Keratinocytes / Q000502:physiology; D007633:Keratins / Q000378:metabolism; D013997:Time Factors; D014945:Wound Healing
chemical_list: D024141:Collagen Type IV; D053547:Keratin-14; D007633:Keratins