title: Genetic and comparative transcriptome analysis of bromodomain factor 1 in the salt stress response of Saccharomyces cerevisiae.
abstract: The Saccharomyces cerevisiae BDF1 gene, which encodes a bromodomain-containing transcription factor, was previously isolated by transposon mutugenesis in a screen for salt-sensitive mutants. However, the salt stress response mechanism regulated by bromodomain transcription factor 1 protein (Bdf1p) remains poorly understood. In this report, genetic analysis indicated that the salt sensitivity of the BDF1 deletion mutant was suppressed by increased gene dosage of its homologous gene BDF2. Furthermore, comparative transcriptome analysis revealed that the differences in transcriptional response between the wild type and the bdf1Delta mutant in the presence of salt stress (0.6 mol/L NaCl, 45 min) were mainly related to cell-wall biosynthesis, the mitochondria, and several unknown genes. Our results provided further information about the regulatory mechanism involved in the salt stress response and adds new insight for understanding the biological functional of bromdomain-containing proteins in cellular processes.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, P.R. China, bxm@sdu.edu.cn.
authors.forename: Xiangyong
authors.initials: X
authors.lastname: Liu
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Zhang
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.forename: Liangyu
authors.initials: L
authors.lastname: Liu
authors.forename: Ming
authors.initials: M
authors.lastname: Lei
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Bao
mesh_terms: D018628:Gene Dosage; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D005800:Genes, Fungal; D005838:Genotype; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D012965:Sodium Chloride / Q000494:pharmacology*; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects*; D000092468:Bromodomain Containing Proteins
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D012965:Sodium Chloride; D014157:Transcription Factors; C091342:BDF1 protein, S cerevisiae; D000092468:Bromodomain Containing Proteins