title: Use of the kinetic plot method to analyze commercial high-temperature liquid chromatography systems. II. Practically constrained performance comparison.
abstract: Using a set of experimentally determined plate height data obtained on three commercial high-temperature HPLC supports, and evaluating their isocratic separation speed potential under the application of a set of instrumental constraints, a qualitative map of the practically achievable critical pair separation speed potential of high-temperature HPLC has been established. The obtained data show that the gain in separation speed is more strongly affected by the instrumental limitations in the high-temperature range than it is for the low temperatures. For the presently considered case of alkylbenzene separations, the potential gain in analysis time that can be obtained by going from T=30 to 120 degrees C in the presence of a typical set of instrumental limitations nevertheless remains of the order of a factor of 2-4. The study also shows that improvements on the instrumentation side (increased detector frequency, pumping flow rate, smaller extra-column volumes, ...) are indispensable to fully benefit from the high temperature advantages for all separations requiring less than 10,000 effective theoretical plates.
authors.affiliation: Vrije Universiteit Brussel, Department of Chemical Engineering (CHIS-TW), Pleinlaan 2, 1050 Brussels, Belgium.
authors.forename: D
authors.initials: D
authors.lastname: Clicq
authors.forename: S
authors.initials: S
authors.lastname: Heinisch
authors.forename: J L
authors.initials: JL
authors.lastname: Rocca
authors.forename: D
authors.initials: D
authors.lastname: Cabooter
authors.forename: P
authors.initials: P
authors.lastname: Gzil
authors.forename: G
authors.initials: G
authors.lastname: Desmet
mesh_terms: D000465:Algorithms; D000473:Alkanes / Q000032:analysis / Q000737:chemistry; D001554:Benzene / Q000032:analysis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007700:Kinetics; D015203:Reproducibility of Results; D013696:Temperature
chemical_list: D000473:Alkanes; D001554:Benzene