title: [Concentration of thyroid stimulating hormone and activity of N-acetyl-beta-D-hexosaminidase and its isoenzymes, in serum of patients with thyroid cancer].
abstract: Thyroid cancer consists 1% of all malignant neoplasms. It is not known interrelationship between concentration of TSH in blood serum and condition of thyroid cancer. Thyroid cancer is difficult for diagnosis and differentiation. Therefore it is necessary to search for biochemical markers helpful in diagnostics of thyroid cancer. Significant increase in activity of N-acetyl-beta-D-hexosaminidase and its isoenzymes A and B in serum of patients with neoplasms of kidney and pancreas suggest approporiateness of evaluation of HEX and its isoenzymes in diagnostics of thyroid cancer.
  
  
  OBJECTIVE
  of the study--evaluation of TSH concentration and activity of HEX and its isoenzymes A and B, in serum of patients with thyroid cancer.
  
  
  METHODS
  Blood was taken from 7 patients with thyroid cancer (6 men and 1 woman). Control consisted of 7 healthy men. In blood serum concentration of TSH was determined with immunoenzymatic method on analyzer Axsym of Abbott and expressed in microU/mL. The activity of HEX and its isoenzymes A and B was determined by method of Chatterjee et al., as modified by Zwierz et.al. Determination of HEX was performed on microplate reader ELX800 BIO-TEK. Activity of HEX, HEX A and B was expressed in pKat/mL, and specific activity in pKat/mg protein). Protein was determined by biuret method and results were expressed in mg/mL.
  
  
  RESULTS
  Concentration of HEX A activity in serum of thyroid cancer patients is significantly higherin comparison to healthymen (p = 0.0191). Also specific activity of HEX A in serum of thyroid cancer patients is significantly higher in comparison to healthy men (p = 0.0393).
  
  
  CONCLUSIONS
  1. Determination of TSH concentration in serum of thyroid cancer before the operation may confirm euthyreosis. 2. Determination of HEX A activity in serum may be helpful in diagnostics of thyroid cancer.
authors.affiliation: Department of Pharmaceutical Biochemistry, Medical University of Białystok, Poland. kzwierz@amb.edu.pl
authors.forename: Piotr
authors.initials: P
authors.lastname: Zwierz
authors.forename: Sławomir D
authors.initials: SD
authors.lastname: Szajda
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Snarska
authors.forename: Zbigniew B
authors.initials: ZB
authors.lastname: Supronowicz
authors.forename: Paweł
authors.initials: P
authors.lastname: Zawadzki
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Zwierz
authors.forename: Fabian
authors.initials: F
authors.lastname: Kamińsk
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D054818:Hexosaminidase A; D006801:Humans; D007527:Isoenzymes / Q000097:blood; D008297:Male; D008875:Middle Aged; D044967:Serum / Q000201:enzymology*; D013964:Thyroid Neoplasms / Q000097:blood / Q000201:enzymology*; D013972:Thyrotropin / Q000097:blood*; D001619:beta-N-Acetylhexosaminidases / Q000097:blood*
chemical_list: D014408:Biomarkers, Tumor; D007527:Isoenzymes; D013972:Thyrotropin; D054818:Hexosaminidase A; D001619:beta-N-Acetylhexosaminidases