Application of high-speed counter-current chromatography for the isolation of 9'-cis-neoxanthin from fresh spinach.
Preparative HSCCC (high-speed counter-current chromatography) could be applied for the isolation of 9'-cis-neoxanthin from a crude carotenoid extract of fresh spinach leaves. The separation was performed on a Pharma-Tech Research Corp. CCC 1000 with a solvent system composed of hexane:ethanol:water at a volume ratio of 5:5:4.5 at a flow rate of 3 mL/min and at 850 rpm, using the lower phase as mobile phase. 9'-cis-neoxanthin with a purity of up to 94% could be obtained with a single HSCCC purification step of the crude carotenoid extract.
Baldermann
Susanne
S
Institute of Food Chemistry, Technical University of Braunschweig, Schleinitzstrasse 20, 38106 Braunschweig, Germany. s.baldermann@tu-bs.de
Reinhard
Annika
A
Köhler
Nils
N
Fleischmann
Peter
P
D003377:Countercurrent Distribution / Q000379:methods*; D015394:Molecular Structure; D015203:Reproducibility of Results; D018724:Spinacia oleracea / Q000737:chemistry*; D024341:Xanthophylls / Q000737:chemistry / Q000302:isolation & purification*
D024341:Xanthophylls; C011947:neoxanthin