title: Biodegradation of nicotine from tobacco waste extract by Ochrobactrum intermedium DN2.
abstract: Ochrobactrum intermedium DN2 was used to degrade nicotine in tobacco waste extracts. The optimal temperature and pH of nicotine degradation by strain DN2 was 30-37 degrees C and 7.0, respectively. Under these optimal conditions, the average degradation rate of nicotine in a 30L fed-batch culture was 140.5 mg 1(-1) h(-1). The results of this study indicate that strain DN2 may be useful for reducing the nicotine content of reconstituted tobacco.
authors.affiliation: College of Food science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing, People's Republic of China.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Yuan
authors.forename: Z X
authors.initials: ZX
authors.lastname: Lu
authors.forename: L J
authors.initials: LJ
authors.lastname: Huang
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: F X
authors.initials: FX
authors.lastname: Lu
authors.forename: X M
authors.initials: XM
authors.lastname: Bie
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Teng
authors.forename: Q
authors.initials: Q
authors.lastname: Lin
mesh_terms: D001673:Biodegradation, Environmental; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D009538:Nicotine / Q000378:metabolism*; D018722:Nicotinic Agonists / Q000378:metabolism; D042041:Ochrobactrum / Q000378:metabolism*; D013696:Temperature; D014026:Nicotiana / Q000737:chemistry*
chemical_list: D007220:Industrial Waste; D018722:Nicotinic Agonists; D009538:Nicotine