title: Subunits of voltage-gated calcium channels in murine spiral ganglion cells.
abstract: CONCLUSIONS
  The results show that alpha1D, alpha1E, alpha2/delta, beta1, and beta3 subunits are expressed in spiral ganglion cells (SGCs), and the coexpression of alpha1D and alpha1E suggests the presence of L-type and R-type calcium channels in mammalian SGCs.
  
  
  OBJECTIVE
  To investigate the types of subunits of voltage-gated calcium channels in SGCs of the mouse.
  
  
  METHODS
  SGCs were isolated from cochleae of neonatal mice and cultured for 24 h. Total RNA was extracted from cultured cells. After reverse transcription, the resulting cDNA was amplified by PCR with primers targeted to nucleotide sequences corresponding to seven different calcium channel subunits. The types of calcium channel subunits were identified by PCR analysis and nucleotide sequencing.
  
  
  RESULTS
  RT-PCR showed the strong and consistent amplification of alpha1D, alpha1E, alpha2/delta, beta1, and beta3 subunits from the mRNA of SGCs, and nucleotide sequencing confirmed the identity of mouse cochlear subunit cDNAs.
authors.affiliation: Department of Otolaryngology and Head & Neck Surgery of the Second Xiangya Hospital and Institute of Otology, Changsha, People's Republic of China. huaxie@public.cs.hn.cn
authors.forename: Dinghua
authors.initials: D
authors.lastname: Xie
authors.forename: Peng
authors.initials: P
authors.lastname: Hu
authors.forename: Zi'an
authors.initials: Z
authors.lastname: Xiao
authors.forename: Weijing
authors.initials: W
authors.lastname: Wu
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.forename: Kun
authors.initials: K
authors.lastname: Xia
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015220:Calcium Channels / Q000235:genetics* / Q000378:metabolism*; D020746:Calcium Channels, L-Type / Q000235:genetics / Q000378:metabolism; D020908:Calcium Channels, R-Type / Q000235:genetics* / Q000378:metabolism*; D027682:Cation Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D003051:Cochlea / Q000166:cytology / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D051379:Mice; D021122:Protein Subunits / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013136:Spiral Ganglion / Q000166:cytology* / Q000378:metabolism*
chemical_list: C512066:CACNA2D1 protein, mouse; C498916:Cacna1e protein, mouse; C492923:Cacnb1 protein, mouse; D015220:Calcium Channels; D020746:Calcium Channels, L-Type; D020908:Calcium Channels, R-Type; D027682:Cation Transport Proteins; D018076:DNA, Complementary; D021122:Protein Subunits; D012333:RNA, Messenger