Inhibition of lens-aldose reductase activity by brazilin and haematoxylin.
Brazilin and haematoxylin, plant pigments, were examined for their effects on the Bovine-Lens aldose reductase (LAR)-activity. About 50% inhibition was observed in a concentration of 10 (-4) M-brazilin and 10 (-4) M-haematoxylin, and above 95% inhibition was observed in a concentration of 10 (-3) M-brazilin and 10 (-3)M-haematoxylin. In order to determine the type of inhibition, kinetic studies were also conducted with brazilin and haematoxylin, in which both were found to be noncompetitive inhibitors.
Moon
C K
CK
College of Pharmacy, Seoul National University, San 56-1, Sinlim-Dong, Kwanak-Gu, Seoul 151, Korea.
Yun
Y P
YP
Lee
J H
JH
Wagner
H
H
Shin
Y S
YS