title: [Studies on impact of temperature and weight in Whitmania pigra bred].
abstract: OBJECTIVE
  To determine the best spawning temperature and weight of Whitmania pigra and the hatching temperature of its eggs.
  
  
  METHODS
  Observe the state of W. pigra spawning and hatching were observed under different temperatures and weights conditions.
  
  
  CONCLUSIONS
  25 degrees C is the most appropriate temperature for W. pigra spawning and incubation, the best spawning weight of W. pigra was around 20 g. The temperature influenced the lost weight after spawning significantly and was proportion to the lost weight. The body weight showed no substantial influence to the lost weight after spawning. The egg weight of W. pigra which body weight was below 2.0 g was proportion to the hatching rate. The same relationship was not observed in W. pigra over 2.0 g.
authors.affiliation: Institute of Chinese medicinal materials, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Hong-Zhuan
authors.initials: HZ
authors.lastname: Shi
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.forename: Qiao-Sheng
authors.initials: QS
authors.lastname: Guo
mesh_terms: D000818:Animals; D001835:Body Weight; D001947:Breeding / Q000379:methods; D005260:Female; D007865:Leeches / Q000254:growth & development* / Q000502:physiology; D008297:Male; D010063:Ovum / Q000254:growth & development; D013696:Temperature