title: Effects of spilled oil on bacterial communities of mediterranean coastal anoxic sediments chronically subjected to oil hydrocarbon contamination.
abstract: The effects of spilled oil on sedimentary bacterial communities were examined in situ at 20 m water depth in a Mediterranean coastal area. Sediment collected at an experimental site chronically subjected to hydrocarbon inputs was reworked into PVC cores with or without a massive addition of crude Arabian light oil ( approximately 20 g kg(-1) dry weight). Cores were reinserted into the sediment and incubated in situ at the sampling site (20 m water depth) for 135 and 503 days. The massive oil contamination induced significant shifts in the structure of the indigenous bacterial communities as shown by ribosomal intergenic spacer analysis (RISA). The vertical heterogeneity of the bacterial communities within the sediment was more pronounced in the oiled sediments particularly after 503 days of incubation. Response to oil of the deeper depth communities (8-10 cm) was slower than that of superficial depth communities (0-1 and 2-4 cm). Analysis of the oil composition by gas chromatography revealed a typical microbial alteration of n-alkanes during the experiment. Predominant RISA bands in oiled sediments were affiliated to hydrocarbonoclastic bacteria sequences. In particular, a 395-bp RISA band, which was the dominant band in all the oiled sediments for both incubation times, was closely related to hydrocarbonoclastic sulfate-reducing bacteria (SRB). These bacteria may have contributed to the main fingerprint changes and to the observed biodegradation of n-alkanes. This study provides useful information on bacterial dynamics in anoxic contaminated infralittoral sediments and highlights the need to assess more precisely the contribution of SRB to bioremediation in oil anoxic contaminated areas.
authors.affiliation: Laboratoire de Microbiologie, de Géochimie et d'Ecologie Marines, CNRS-UMR 6117, Centre d'Océanologie de Marseille, Cedex 9, Case 901, Campus de Luminy, Marseille, 13288, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Miralles
authors.forename: David
authors.initials: D
authors.lastname: Nérini
authors.forename: Claude
authors.initials: C
authors.lastname: Manté
authors.forename: Monique
authors.initials: M
authors.lastname: Acquaviva
authors.forename: Pierre
authors.initials: P
authors.lastname: Doumenq
authors.forename: Valérie
authors.initials: V
authors.lastname: Michotey
authors.forename: Sylvie
authors.initials: S
authors.lastname: Nazaret
authors.forename: Jean Claude
authors.initials: JC
authors.lastname: Bertrand
authors.forename: Philippe
authors.initials: P
authors.lastname: Cuny
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D017753:Ecosystem; D004784:Environmental Monitoring; D019015:Geologic Sediments / Q000737:chemistry / Q000382:microbiology*; D006838:Hydrocarbons / Q000032:analysis; D008522:Mediterranean Sea; D008969:Molecular Sequence Data; D010100:Oxygen / Q000032:analysis; D010578:Petroleum; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014873:Water Pollutants / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer; D006838:Hydrocarbons; D010578:Petroleum; D012336:RNA, Ribosomal, 16S; D014873:Water Pollutants; D010100:Oxygen