title: Preservation of the pHi during ischemia via PKC by intermittent hypoxia.
abstract: In intermittent hypoxia adaptation (IHA) rat cardiomyocytes, the relationship between activated protein kinase C and intracellular acidification regulation during ischemia-reperfusion (I/R) was tested. Using [H(+)] indicator BCECF-AM, we analyzed the alterations of intracellular pH (pH(i)) in normoxia and IHA rat cardiomyocytes during I/R. With the time of ischemia, the pH(i) decreased progressively in normal cardiomyocytes, but fewer alterations in IHA myocytes. Treatment of IHA and normoxia cardiomyocytes with 5 microM chelerythrine delayed the pH(i) recovery during post-ischemia. In contrast, the application of 1 microM phorbol 12-myristate 13-acetate in normoxia cardiomyocytes preserved the pH(i) during I/R, which was similar to that in IHA cardiomyocytes. Our data suggest that the stable PKC activation might contribute to preservation of the pH(i), which may be beneficial to maintain cardiac function during I/R in IHA rats.
authors.affiliation: Laboratory of Molecular Cardiology, Institute of Health Sciences, Shanghai Jiao Tong University School of Medicine (SJTUSM) [corrected] Shanghai 200025, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Wei-Zhong
authors.initials: WZ
authors.lastname: Zhu
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Yu
authors.forename: Ang
authors.initials: A
authors.lastname: Guo
authors.forename: Huang-Tian
authors.initials: HT
authors.lastname: Yang
authors.forename: Zhao-Nian
authors.initials: ZN
authors.lastname: Zhou
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D004195:Disease Models, Animal; D006863:Hydrogen-Ion Concentration; D000860:Hypoxia / Q000201:enzymology / Q000503:physiopathology*; D007511:Ischemia / Q000503:physiopathology*; D008297:Male; D011493:Protein Kinase C / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015424:Reperfusion
chemical_list: D011493:Protein Kinase C