Clinical experiment of mutant herpes simplex virus HF10 therapy for cancer.
We reviewed our clinical trial using mutant herpes simplex virus "HF10". We have evaluated the safety and effect of HF10 against recurrent breast cancer since 2003 and also applied HF10 to non-resectable pancreatic cancer since 2005. An oncolytic herpes simplex virus type 1, mutant HF10, has been isolated and evaluated for anti-tumor efficacy in syngeneic immunocompetent mouse models. From long time before clinical trial, we have found that the mutant virus can have remarkable potential to effectively treat cancer in experimental studies using animals, and that all of the surviving mice acquire resistance to rechallenge of the tumor cells. A number of studies have shown that HF10 is effective and safe for use in localized or peritoneally disseminated malignant tumors of non-neuronal origin in animals. Pilot studies using HF10 have been initiated in patients with metastatic breast cancer. For each patient, 0.5 ml HF10 diluents at various doses were injected into test nodule, and 0.5 ml sterile saline was injected into a second nodule. All patients were monitored for local and systemic adverse effects, and the nodules were excised 14 days after viral injection for histopathological studies. All patients tolerated the clinical trial well. While no adverse effects occurred, there was cancer cell death and 30-100% regression histopathologically in recurrent breast cancer. As mentioned above, intratumoral injection of mutant herpes simplex virus HF10 for recurrent metastatic breast cancer was safe and effective. Also a trial for non-resectable pancreatic cancer being carried out on the basis of the above result has proved to be innocuous and has been in progress to assess the clinical benefit and enhance the potentiality of HF10 against cancer.
Nakao
A
A
Department of Surgery II, Nagoya University, Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan. nakaoaki@med.nagoya-u.ac.jp
Takeda
S
S
Shimoyama
S
S
Kasuya
H
H
Kimata
H
H
Teshigahara
O
O
Sawaki
M
M
Kikumori
T
T
Kodera
Y
Y
Nagasaka
T
T
Goshima
F
F
Nishiyama
Y
Y
Imai
T
T
D000368:Aged; D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology / Q000628:therapy*; D005260:Female; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D050130:Oncolytic Virotherapy; D010190:Pancreatic Neoplasms / Q000473:pathology / Q000628:therapy*; D012107:Research Design; D016896:Treatment Outcome; D014779:Virus Replication