Mechanochemical kinetics of transcription elongation.
The mechanochemical kinetics of transcription elongation was examined with a combination of theoretical and experimental approaches. The predictive power of a sequence-dependent thermal ratchet model for transcription elongation was tested by establishing model parameters based solely on measurements under chemical perturbations and then directly predicting responses under mechanical perturbations without additional model parameters. Agreement between predicted and measured force-velocity curves provides strong support for a simple mechanochemical coupling mechanism.
Bai
Lu
L
Department of Physics, Laboratory of Atomic and Solid State Physics, Cornell University, Ithaca, New York 14853, USA.
Fulbright
Robert M
RM
Wang
Michelle D
MD
D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry* / Q000378:metabolism*; D007700:Kinetics; D008957:Models, Genetic; D009711:Nucleotides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
D009711:Nucleotides; D012333:RNA, Messenger; D004247:DNA; D012321:DNA-Directed RNA Polymerases