Identification of RNA binding sequences of Drosophila SR protein DX16.
Dxl6 is a member of the Drosophila melanogaster SR protein family, a group of nuclear proteins that are both essential splicing factors and specific splicing regulators. To get more insight of Dx16 function, we generated the monoclonal antibody against Dx16 and determined its expression pattern and subcellular location. It is mainly expressed in the nucleus of CNS in Drosophila embryos. In order to investigate the RNA-binding specificity of Dxl6, Dxl6-binding RNAs were identified by SELEX screen by using recombinant Dxl6 N-terminus protein as the target. These RNAs contained a consensus motif. Some pre-mRNAs from the corresponding genes showed splicing defects in the Dxl6-P-element insertional mutant fly. These results indicate that Dxl6 has unique functions in the removal of some introns during development.
Yuan
Liudi
L
Department of Genetics and Developmental Biology, Southeast University Medical School, Nanjing 210009, P.R. China.
Zhou
Jing
J
Wan
Yongqi
Y
Sun
Mingkuan
M
Ding
Jie
J
Dou
Fei
F
Xie
Wei
W
D017398:Alternative Splicing / Q000235:genetics; D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D052157:Aptamers, Nucleotide / Q000378:metabolism; D001483:Base Sequence; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism; D008969:Molecular Sequence Data; D009420:Nervous System / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D011485:Protein Binding; D012313:RNA / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D052156:SELEX Aptamer Technique
D000911:Antibodies, Monoclonal; D052157:Aptamers, Nucleotide; D029721:Drosophila Proteins; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; C524292:x16 protein, Drosophila; D012313:RNA