Chemical concentration measurement in blood serum and urine samples using liquid-core optical fiber Raman spectroscopy.
We report measurements of chemical concentrations in clinical blood serum and urine samples using liquid-core optical fiber (LCOF) Raman spectroscopy to increase the collected signal strength. Both Raman and absorption spectra were acquired in the near-infrared region using the LCOF geometry. Spectra of 71 blood serum and 61 urine samples were regressed via partial least squares against reference analyzer values. Significant correlation was found between predicted and reference concentrations for 13 chemicals. Using absorption data to normalize the LCOF enhancement made the results more accurate. The experimental geometry is well suited for high-volume and automated chemical analysis of clear biofluids.
Qi
Dahu
D
The Institute of Optics, University of Rochester, New York 14627, USA.
Berger
Andrew J
AJ
D000465:Algorithms; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D001774:Blood Chemical Analysis / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation* / Q000379:methods; D055100:Optical Fibers; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012996:Solutions; D013048:Specimen Handling / Q000295:instrumentation; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*; D016482:Urinalysis / Q000295:instrumentation* / Q000379:methods
D012996:Solutions