title: A simple method of preparing diverse neoagaro-oligosaccharides with beta-agarase.
abstract: In order to prepare pure and well-defined oligosaccharides from agarose in a rapid and simple manner, an enzymatic degradation method was developed, which includes degradation with either recombinant beta-agarase (EC 3.2.1.81) AgaA or AgaB and gel permeation chromatography. Agarose was degraded with AgaA at the optimized conditions, yielding 47% and 45% of neoagarotetraose and neoagarohexaose, respectively. These neoagaro-oligosaccharides were conveniently separated by consecutive column chromatography on Bio-Gel P2 or P6 and were identified by FACE. The structure of these neoagaro-oligosaccharides was confirmed by MALDI-TOF MS and (13)C NMR spectroscopy.
authors.affiliation: Marine Drug and Food Institute, Ocean University of China, 5 Yushan Road, Qingdao 266003, China.
authors.forename: Jingbao
authors.initials: J
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Han
authors.forename: Xinzhi
authors.initials: X
authors.lastname: Lu
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Fu
authors.forename: Cuiping
authors.initials: C
authors.lastname: Ma
authors.forename: Yan
authors.initials: Y
authors.lastname: Chu
authors.forename: Wengong
authors.initials: W
authors.lastname: Yu
mesh_terms: D002236:Carbohydrate Conformation; D004926:Escherichia coli / Q000201:enzymology; D006026:Glycoside Hydrolases / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000138:chemical synthesis*; D011994:Recombinant Proteins / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D009844:Oligosaccharides; D011994:Recombinant Proteins; D006026:Glycoside Hydrolases; C019624:agarase