The effects of Porphyromonas gingivalis LPS and Actinobacillus actinomycetemcomitans LPS on human dendritic cells in vitro, and in a mouse model in vivo.
Interaction between different bacterial plaque pathogens and dendritic cells may induce different types of T helper (Th) cell response, which is critical in the pathogenesis of periodontitis. In this study we investigated the effects of lipopolysaccharide (LPS) from Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans on human monocyte-derived dendritic cells (Mo-DCs) with respect to co-stimulatory molecule expression, cytokine production and Th cell differentiation. Unlike Escherichia coli and A. actinomycetemcomitans LPS, P. gingivalis LPS induced only low levels of CD40, CD80, HLA-DR and CD83 expression on Mo-DCs. LPS from both bacteria induced considerably lower TNF-alpha and IL-10 than did E. coli LPS. LPS from all three bacteria induced only negligible IL-12 production. In a human mixed-leukocyte reaction, and in an ovalbumin-specific T cell response assay in mice, both types of LPS suppressed IFN-gamma production. In conclusion, stimulation by P. gingivalis LPS and A. actinomycetemcomitans LPS appears to bias Mo-DCs towards Th2 production.
Mahanonda
Rangsini
R
Department of Periodontology, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand. mrangsin@chula.ac.th
Pothiraksanon
Piyawadee
P
Sa-Ard-Iam
Noppadol
N
Yamazaki
Kazuhisa
K
Schifferle
Robert E
RE
Hirunpetcharat
Chakrit
C
Yongvanichit
Kosol
K
Pichyangkul
Sathit
S
D016976:Aggregatibacter actinomycetemcomitans / Q000737:chemistry / Q000276:immunology*; D000818:Animals; D000943:Antigens, Differentiation / Q000276:immunology; D002454:Cell Differentiation / Q000187:drug effects / Q000276:immunology; D002478:Cells, Cultured; D018920:Coculture Techniques; D016207:Cytokines / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D004926:Escherichia coli / Q000737:chemistry / Q000276:immunology; D006801:Humans; D008070:Lipopolysaccharides / Q000737:chemistry / Q000276:immunology* / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D018448:Models, Immunological; D016966:Porphyromonas gingivalis / Q000737:chemistry / Q000276:immunology*; D018418:Th2 Cells / Q000166:cytology / Q000276:immunology*
D000943:Antigens, Differentiation; D016207:Cytokines; D008070:Lipopolysaccharides