[Alpha,beta-poly[(N-hydroxypropyl/aminoethyl)-DL-aspartamide -co-L-lysine]: potential non-viral vehicle for gene delivery].
A series of Poly[aspartic acid-co-L-lysine](PAL) are copolycondensed by DL-aspartice acid and L-lysine with different ratios. Their constructions are identified by the spectra of 1H-NMR, FT-IR, X-Ray). These spectra are proved to have good regularity of these copolymers. alpha,beta-Poly[(N-hydroxypropyl/aminoethyl)-DL-Aspartamide-co-L-lysine] (PHAAL) is synthesized by ring-opening poly [aspartic acid-co-lysine] (PAL). PHAAL has good degradability in the phosphoric acid buffer solution (0.01 M, pH = 7.4) in the enzyme solution (Papain, Trypsine). PHAAL appeared tobe low cytotoxicity in Hela, ECV-304, Bcap37 cell lines, which was quantified by MTT assay. The combination ability of PHAAL with plasmid DNA was evaluated by agarose gel electrophoresis with agarose gel (1.0% w/v) containing ethidium bromide (0.25 microg/ml). The PHAAL with higher ratios of lysine in the copolymers have higher ability of condensing DNA. In summary, PHAAL, the polyaminoacid materials, could be one kind of macromolecule materials tobeused as the non-viral gene vehicle.
Luo
Yin
Y
Hangzhou First People's Hospital, Hangzhou 310006, China. adoer@sina.com
Hou
Si'en
S
Gao
Zhongming
Z
Tang
Guping
G
D001224:Aspartic Acid / Q000138:chemical synthesis* / Q000737:chemistry / Q000633:toxicity; D001704:Biopolymers / Q000737:chemistry / Q000633:toxicity; D042783:Endothelial Cells / Q000166:cytology; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000138:chemical synthesis / Q000737:chemistry / Q000633:toxicity; D006367:HeLa Cells; D006801:Humans; D008422:Materials Testing; D000091402:Prohibitins; D014470:Umbilical Cord / Q000166:cytology
D001704:Biopolymers; C000717331:PHB2 protein, human; D000091402:Prohibitins; C499773:alpha,beta-poly(aspartic acid); D001224:Aspartic Acid