[The effect of cyclin D2 shRNA on the proliferation and apoptosis of LP-1 cell].
OBJECTIVE
To construct cyclin D2 (CCND2) short hairpin RNA ( shRNA) plasmid for repressing the expression of CCND2 in human myeloma cell line LP-1,and to detect its effect on the proliferation and apoptosis of LP-1 cell.


METHODS
A CCND2 shRNA model was constructed and cloned into plasmid pGensil-2, then the plasmid was transfected into LP-1 cell in vitro. The CCND2 expression cell proliferation, cell cycle and cell apoptosis of the transfected LP-1 cells were studied by RT-PCR, trypanosome staining, flow cytometry and annexin V assay.


RESULTS
The transfection efficiency of LP-1 cell was 34. 2%. In the transfected LP-1 cell CCND2 mRNA expression was reduced significantly, the cell growth was inhibited significantly and the cell cycle was partly arrested in G, phase. The apoptosis rate of the transfected LP-1 cell after 72 h was (25.7+/-4.8)%.


CONCLUSIONS
The inhibition of CCND2 in LP-1 cells could inhibit the cell growth and induce cell apoptosis. CCND2 maybe a new therapeutic target.
Guo
Cha
C
Department of Hematology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
Hou
Jian
J
Chen
Yu-bao
YB
Jin
Hui-yan
HY
Fu
Wei-jun
WJ
Yuan
Zhen-gang
ZG
Wang
Dong-xing
DX
D017209:Apoptosis; D049109:Cell Proliferation; D056742:Cyclin D2; D016213:Cyclins / Q000235:genetics*; D006801:Humans; D034622:RNA Interference; D012330:RNA, Double-Stranded; D034741:RNA, Small Interfering; D014162:Transfection; D014407:Tumor Cells, Cultured
C080336:CCND2 protein, human; D056742:Cyclin D2; D016213:Cyclins; D012330:RNA, Double-Stranded; D034741:RNA, Small Interfering