title: IL-27 induces the production of IgG1 by human B cells.
abstract: It has been reported that IL-27 specifically induces the production of IgG2a by mouse B cells and inhibits IL-4-induced IgG1 synthesis. Here, we show that human naïve cord blood expresses a functional IL-27 receptor, consisting of the TCCR and gp130 subunits, although at lower levels as compared to naïve and memory splenic B cells. IL-27 does not induce proliferative responses and does not increase IgG1 production by CD19(+)CD27(+) memory B cells. However, it induces a low, but significant production of IgG1 by naïve CD19(+)CD27(-)IgD(+)IgG(-) spleen and cord blood B cells, activated via CD40, whereas it has no effect on the production of the other IgG subclasses. In addition, IL-27 induces the differentiation of a population of B cells that express high levels of CD38, in association with a down-regulation of surface IgD expression, and that are surface IgG(+/int), CD20(low), CD27(high), indicating that IL-27 promotes isotype switching and plasma cell differentiation of naive B cells. However, as compared to the effects of IL-21 and IL-10, both switch factors for human IgG1 and IgG3, those of IL-27 are modest and regulate exclusively the production of IgG1. Finally, although IL-27 has no effect on IL-4 and anti-CD40-induced Cepsilon germline promoter activity, it up-regulates IL-4-induced IgE production by naive B cells. These results point to a partial redundancy of switch factors regulating the production of IgG1 in humans, and furthermore indicate the existence of a common regulation of the human IgG1and murine IgG2a isotypes by IL-27.
authors.affiliation: Inserm U454, CHU Arnaud de Villeneuve, 371 av. Doyen Gaston-Giraud, 34295 Montpellier Cedex 05, France.
authors.forename: Amel
authors.initials: A
authors.lastname: Boumendjel
authors.forename: Lina
authors.initials: L
authors.lastname: Tawk
authors.forename: René de Waal
authors.initials: Rde W
authors.lastname: Malefijt
authors.forename: Vera
authors.initials: V
authors.lastname: Boulay
authors.forename: Hans
authors.initials: H
authors.lastname: Yssel
authors.forename: Jérôme
authors.initials: J
authors.lastname: Pène
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D018941:Antigens, CD19 / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D019013:CD40 Antigens / Q000276:immunology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology*; D007132:Immunoglobulin Isotypes / Q000276:immunology; D016753:Interleukin-10 / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D007378:Interleukins / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000276:immunology; D000098546:Interleukin-21
chemical_list: D000911:Antibodies, Monoclonal; D018941:Antigens, CD19; D019013:CD40 Antigens; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007378:Interleukins; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D016753:Interleukin-10; D015847:Interleukin-4; D000098546:Interleukin-21