Actin organization and root hair development are disrupted by ethanol-induced overexpression of Arabidopsis actin interacting protein 1 (AIP1).
* Actin organization and dynamics are essential for cell division, growth and cytoplasmic streaming. Here we analyse the effects of the overexpression of Actin Interacting Protein 1 (AIP1) on Arabidopsis development. * Arabidopsis plants were transformed with an ethanol-inducible AIP1 construct and the characteristics of these plants were analysed after induction. * When AIP1 was increased to approx. 90% above wild-type values, root hair development and actin organization in all cell types examined were disrupted. * Our data demonstrate that AIP1 is a key regulator of actin organization and that its regulation is essential for normal plant cell morphogenesis.
Ketelaar
Tijs
T
The Integrative Cell Biology Laboratory, School of Biological and Biomedical Sciences, University of Durham, South Road, Durham DH1 3LE, UK.
Allwood
Ellen G
EG
The Integrative Cell Biology Laboratory, School of Biological and Biomedical Sciences, University of Durham, South Road, Durham DH1 3LE, UK.
Hussey
Patrick J
PJ
The Integrative Cell Biology Laboratory, School of Biological and Biomedical Sciences, University of Durham, South Road, Durham DH1 3LE, UK.
D000199:Actins / Q000502:physiology*; D017360:Arabidopsis / Q000166:cytology / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D003599:Cytoskeleton / Q000502:physiology; D000431:Ethanol; D018517:Plant Roots / Q000166:cytology / Q000254:growth & development*; D030821:Plants, Genetically Modified
C408685:AIP1 protein, Arabidopsis; D000199:Actins; D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D000431:Ethanol