title: Improvement of a beta-glucanase activity test by taking into account the batch reactor balance of the test system.
abstract: Activity tests of enzymes are often applied for determining their concentration. In the easiest case, just one product concentration is measured after a given time. This often leads to nonlinear dependences of the apparent activity with enzyme protein concentration. A general solution of this problem consists in using the balance equation of the assay system, which commonly represents a batch reactor. Here, the balance equation of the batch for a general Michaelis Menten-type reaction kinetics is used as the calibration function. The correlation of the apparent activity and enzyme concentration was established by capturing the enzyme by means of metal chelate interaction owing to a hexahistidine tag attached to the beta-glucanase.
authors.affiliation: Faculty of Technology, Fermentation Engineering, Bielefeld University, Bielefeld 33594, Germany.
authors.forename: Usama
authors.initials: U
authors.lastname: Beshay
authors.forename: Gerd
authors.initials: G
authors.lastname: Miksch
authors.forename: Erwin
authors.initials: E
authors.lastname: Flaschel
mesh_terms: D019149:Bioreactors / Q000382:microbiology*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D004789:Enzyme Activation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004926:Escherichia coli / Q000201:enzymology*; D006026:Glycoside Hydrolases / Q000032:analysis* / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D006026:Glycoside Hydrolases; C028020:licheninase