[Isolation and characterization of human colorectal cancer cell subline with unique metastatic potential in the liver].
OBJECTIVE
To isolate a subclone from human colorectal cancer cell line SW480 with unique metastatic potential in the liver.


METHODS
Human colorectal cancer cells SW480 were implanted into BALB/c nude mice, and the hepatic metastatic lesions were harvested, and the tumor tissue blocks were re-implantated into nude mice for a second round of in vivo selection. The same procedure was repeated 5 times until a new cell subline, designated as SW480/M5, was established, whose biological behaviors was investigated both in vivo and in vitro.


RESULTS
After orthotopic implantation of the tumor tissue into the colon of nude mouse for 28 days, widespread loco-regional and distant metastases occurred in mice inoculated with SW480 tissue, and those inoculated with SW480/M5 tissue had hepatic metastasis only. Compared with the parental cells, SW480/M5 cells were characterized by greater clonality, reproductive activity, motor ability, invasiveness, and weaker adhesive capacity to fibronectin.


CONCLUSIONS
A human colorectal cancer cell subline with unique liver metastatic potential has been established by in vivo selection to serve as a new model for study ing colorectal cancer metastasis.
Zhang
Yan-Fei
YF
Department of Pathology, Southern Medical University, Key Lab for Molecular Tumor Pathology of Guangdong Province, Guangzhou 510515, China. yanfei@fimmu.com
Liu
Li
L
Ding
Yan-Qing
YQ
D000818:Animals; D015179:Colorectal Neoplasms / Q000473:pathology*; D005260:Female; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000556:secondary*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured