Intravenous delivery of autologous mesenchymal stem cells limits infarct size and improves left ventricular function in the infarcted porcine heart.
Systemic delivery of bone marrow-derived mesenchymal stem cells (MSCs) is a noninvasive approach for myocardial repair. We aimed to test this strategy in a pig model of myocardial infarction. Pigs (n = 8) received autologous MSCs (1 x 10(6)/kg body weight) labeled with fluorescent dye 48 h post proximal left anterior descending artery (LAD) occlusion. Hemodyamics, infarct size, and myocardial function were assessed at baseline and after 1 month. Morphologic analysis revealed that labeled MSCs migrated in the peri-infarct region, resulting in smaller infarct size (32 +/- 7 vs. 19 +/- 7%, p = 0.01), higher fractional area shortening (23 +/- 3 vs. 34.0 +/- 7%, p = 0.001), lower left ventricular end diastolic pressure (18.7 +/- 5 vs. 10.2 +/- 4 mmHg, p = 0.02) and higher +dp/dt (4,570 +/- 540 vs. 6,742 +/- 700 mmHg/s, p = 0.03) during inotropic stimulation. Systemic intravenous delivery of MSCs to pigs limits myocardial infarct size and is an attractive approach for tissue repair.
Krause
Ulf
U
Department of Hematology, University of Heidelberg, Heidelberg, Germany.
Harter
Christoph
C
Seckinger
Anja
A
Wolf
David
D
Reinhard
Adrian
A
Bea
Florian
F
Dengler
Thomas
T
Hardt
Stefan
S
Ho
Anthony
A
Katus
Hugo A
HA
Kuecherer
Helmut
H
Hansen
Alexander
A
D000818:Animals; D004195:Disease Models, Animal; D006321:Heart / Q000502:physiology; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D009203:Myocardial Infarction / Q000473:pathology* / Q000503:physiopathology / Q000628:therapy*; D009206:Myocardium / Q000473:pathology; D013552:Swine; D014182:Transplantation, Autologous; D016277:Ventricular Function, Left