title: Effect of experimental hypercholesterolaemia on K+ channel alpha-subunit mRNA levels in rabbit hearts.
abstract: We investigated the effect of dietary cholesterol on gene transcription of delayed rectifier (I(Kr) - ERG1 and I(Ks) - KvLQT1) and transient outward (I(to,fast) - Kv4.2 and Kv4.3) potassium channel subunits in rabbit hearts using real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR). While the level of Kv4.3 mRNA did not change, both Kv4.2 and ERG1 mRNAs were downregulated, whereas the level of KvLQT1 was increased in hypercholesterolaemic rabbits, indicating that hypercholesterolaemia altered ventricular K(+) channel alpha-subunit gene transcription.
authors.affiliation: Department of Pharmacology and Pharmacotherapy, Medical and Health Science Center, University of Debrecen, Hungary. angelika.varga@king.pharmacol.dote.hu
authors.forename: Angelika
authors.initials: A
authors.lastname: Varga
authors.forename: Péter
authors.initials: P
authors.lastname: Bagossi
authors.forename: József
authors.initials: J
authors.lastname: Tözsér
authors.forename: Barna
authors.initials: B
authors.lastname: Peitl
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Szilvássy
mesh_terms: D000818:Animals; D002791:Cholesterol, Dietary / Q000008:administration & dosage*; D000072237:ERG1 Potassium Channel; D051638:Ether-A-Go-Go Potassium Channels; D015870:Gene Expression / Q000187:drug effects; D006937:Hypercholesterolemia / Q000209:etiology / Q000503:physiopathology; D051657:KCNQ1 Potassium Channel / Q000235:genetics; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D024642:Potassium Channels, Voltage-Gated / Q000235:genetics*; D021122:Protein Subunits / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051672:Shal Potassium Channels / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D002791:Cholesterol, Dietary; D000072237:ERG1 Potassium Channel; D051638:Ether-A-Go-Go Potassium Channels; D051657:KCNQ1 Potassium Channel; D024642:Potassium Channels, Voltage-Gated; D021122:Protein Subunits; D012333:RNA, Messenger; D051672:Shal Potassium Channels