title: Okara, soybean residue, prevents obesity in a diet-induced murine obesity model.
abstract: We examined the effect of okara on the prevention of obesity in mice. A modified AIN-76 diet with a high fat content (14.1% of crude fat) was used as a basal diet. Male ICR mice were fed ad libitum with the basal diet or a dried okara-supplemented basal diet (10, 20, or 40%) for 10 weeks. The okara intake dose-dependently suppressed the development of body weight and epididymal white adipose tissue (EWAT), and prevented an increase of plasma lipids, including total cholesterol, LDL cholesterol, and non-esterified fatty acid. The okara intake also prevented steatosis in the liver. Real-time RT-PCR revealed that the okara intake induced down-regulation of the fatty acid synthetase gene and up-regulation of the cholesterol 7 alpha-hydroxylase (CYP7A1) gene in the liver. We also found that the okara intake caused a marked reduction in the expression of leptin and TNF-alpha genes in EWAT. Our results suggest that okara is beneficial in preventing obesity.
authors.affiliation: Gifu Prefectural Research Institute for Bioengineering, Department of Applied Microbiology, Minokamo, Gifu, Japan. matsumoto@bio.rd.pref.gifu.jp
authors.forename: Kenji
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Shin-ichiro
authors.initials: S
authors.lastname: Yokoyama
mesh_terms: D052436:Adipose Tissue, White / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002784:Cholesterol / Q000097:blood; D002790:Cholesterol 7-alpha-Hydroxylase / Q000378:metabolism; D008078:Cholesterol, LDL / Q000097:blood; D004032:Diet; D064429:Fatty Acid Synthases / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005234:Fatty Liver / Q000517:prevention & control; D005786:Gene Expression Regulation / Q000187:drug effects; D020738:Leptin / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008820:Mice, Obese; D009765:Obesity / Q000188:drug therapy* / Q000517:prevention & control; D010940:Plant Proteins / Q000494:pharmacology*; D011134:Polysaccharides / Q000494:pharmacology*; D045730:Soy Foods; D013025:Glycine max / Q000737:chemistry*
chemical_list: D008078:Cholesterol, LDL; D005230:Fatty Acids, Nonesterified; D020738:Leptin; D010940:Plant Proteins; D011134:Polysaccharides; C412882:okara, Glycine max; D002784:Cholesterol; D002790:Cholesterol 7-alpha-Hydroxylase; C502772:Cyp7a1 protein, mouse; D064429:Fatty Acid Synthases