Electrostatic ligand coatings of nanoparticles enable ligand-specific gene delivery to human primary cells.
A general method of coating polymer/DNA nanoparticles was developed. Peptide coated nanoparticles were found to have favorable biophysical characteristics including small particle size, near-neutral zeta potential, and stability in serum. At appropriate formulation conditions including near-neutral charge ratio, the coated nanoparticles enabled effective ligand-specific gene delivery to human primary endothelial cells in serum-containing media. As this nanoparticulate drug delivery system has high efficacy, ligand-based specificity, biodegradability, and low cytotoxicity, it may be potentially useful in several clinical applications.
Green
Jordan J
JJ
Division of Biological Engineering, Department of Chemical Engineering, and Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Chiu
Eugene
E
Leshchiner
Elizaveta S
ES
Shi
Julie
J
Langer
Robert
R
Anderson
Daniel G
DG
D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004247:DNA / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D016503:Drug Delivery Systems / Q000379:methods*; D018274:Electroporation / Q000379:methods; D042783:Endothelial Cells / Q000378:metabolism*; D018390:Gene Targeting / Q000379:methods; D018014:Gene Transfer Techniques; D006801:Humans; D008024:Ligands; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D010455:Peptides / Q000737:chemistry*; D055672:Static Electricity; D014162:Transfection / Q000379:methods*
D020099:Coated Materials, Biocompatible; D008024:Ligands; D010455:Peptides; D004247:DNA