In vivo coherent anti-Stokes Raman scattering imaging of sciatic nerve tissue.
We report in vivo nonlinear optical imaging of mouse sciatic nerve tissue by epidetected coherent anti-Stokes Raman scattering and second harmonic generation microscopy. Following a minimally invasive surgery to open the skin, coherent anti-Stokes Raman scattering imaging of myelinated axons and second harmonic generation imaging of the surrounding collagen fibres were demonstrated with high signal-to-background ratio, three-dimensional spatial resolution, and no need for labelling. The underlying contrast mechanisms of in vivo coherent anti-Stokes Raman scattering were explored by three-dimensional imaging of fat cells that surround the nerve. The epidetected coherent anti-Stokes Raman scattering signals from the nerve tissues were found to arise from interfaces as well as back reflection of forward coherent anti-Stokes Raman scattering.
Huff
T B
TB
Department of Chemistry,Purdue University, West Lafayette, IN 47907, USA.
Cheng
J-X
JX
D017667:Adipocytes / Q000166:cytology; D000818:Animals; D003094:Collagen / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D051379:Mice; D008807:Mice, Inbred BALB C; D008853:Microscopy / Q000295:instrumentation / Q000379:methods; D009186:Myelin Sheath / Q000648:ultrastructure; D017711:Nonlinear Dynamics; D012584:Sciatic Nerve / Q000033:anatomy & histology* / Q000378:metabolism; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*
D003094:Collagen