Role of glycolytically generated ATP for CaMKII-mediated regulation of intracellular Ca2+ signaling in bovine vascular endothelial cells.
The role of glycolytically generated ATP in Ca(2+)/calmodulin-dependent kinase II (CaMKII)-mediated regulation of intracellular Ca(2+) signaling was examined in cultured calf pulmonary artery endothelial (CPAE) cells. Exposure of cells (extracellular Ca(2+) concentration = 2 mM) to glycolytic inhibitors 2-deoxy-D-glucose (2-DG), pyruvate (pyr) + beta-hydroxybutyrate (beta-HB), or iodoacetic acid (IAA) caused an increase of intracellular Ca(2+) concentration ([Ca(2+)](i)). CaMKII inhibitors (KN-93, W-7) triggered a similar increase of [Ca(2+)](i). The rise of [Ca(2+)](i) was characterized by a transient spike followed by a small sustained plateau of elevated [Ca(2+)](i). In the absence of extracellular Ca(2+) 2-DG caused an increase in [Ca(2+)](i), suggesting that inhibition of glycolysis directly triggered release of Ca(2+) from intracellular endoplasmic reticulum (ER) Ca(2+) stores. The inositol-1,4,5-trisphosphate receptor (IP(3)R) inhibitor 2-aminoethoxydiphenyl borate abolished the KN-93- and 2-DG-induced Ca(2+) response. Ca(2+) release was initiated in peripheral cytoplasmic processes from which activation propagated as a [Ca(2+)](i) wave toward the central region of the cell. Focal application of 2-DG resulted in spatially confined elevations of [Ca(2+)](i). Propagating [Ca(2+)](i) waves were preceded by [Ca(2+)](i) oscillations and small, highly localized elevations of [Ca(2+)](i) (Ca(2+) puffs). Inhibition of glycolysis with 2-DG reduced the KN-93-induced Ca(2+) response, and vice versa during inhibition of CaMKII 2-DG-induced Ca(2+) release was attenuated. Similar results were obtained with pyr + beta-HB and W-7. Furthermore, 2-DG and IAA caused a rapid increase of intracellular Mg(2+) concentration, indicating a concomitant drop of cellular ATP levels. In conclusion, CaMKII exerts a profound inhibition of ER Ca(2+) release in CPAE cells, which is mediated by glycolytically generated ATP, possibly through ATP-dependent phosphorylation of the IP(3)R.
Aromolaran
Ademuyiwa S
AS
Dept. of Physiology, Loyola University Chicago, 2160 S. First Ave., Maywood, IL 60153, USA.
Zima
Aleksey V
AV
Blatter
Lothar A
LA
D020155:3-Hydroxybutyric Acid / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D001596:Benzylamines / Q000494:pharmacology; D001896:Boron Compounds / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D003847:Deoxyglucose / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism*; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D045604:Extracellular Fluid / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects; D053496:Inositol 1,4,5-Trisphosphate Receptors / Q000037:antagonists & inhibitors / Q000378:metabolism; D019807:Iodoacetic Acid / Q000494:pharmacology; D007700:Kinetics; D008274:Magnesium / Q000378:metabolism; D010766:Phosphorylation; D011651:Pulmonary Artery / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D019289:Pyruvic Acid / Q000378:metabolism; D013449:Sulfonamides / Q000494:pharmacology; D000096926:Benzenesulfonamides
D020155:3-Hydroxybutyric Acid; D000255:Adenosine Triphosphate; D000963:Antimetabolites; D001596:Benzylamines; D001896:Boron Compounds; D002118:Calcium; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D003847:Deoxyglucose; D004791:Enzyme Inhibitors; D053496:Inositol 1,4,5-Trisphosphate Receptors; D019807:Iodoacetic Acid; D008274:Magnesium; D019289:Pyruvic Acid; D013449:Sulfonamides; C072105:KN 93; D000096926:Benzenesulfonamides; C017967:W 7; C109986:2-aminoethoxydiphenyl borate