[Simultaneous determination of six main constituents in Swertia of Qinghai Province and Sichuan Province by HPLC].
OBJECTIVE
To establish a quantitative method of simultaneously determination of swertiamarin, gentiopicroside, mangiferin, swertianolin, isoorientin, 1,8-drihydroxy-3-methoxy-xthanone in Swertia from Qinghai province and Sichuan province by HPLC.


METHODS
The samples were separated on the column of Kromasil C18 (4. 6 mm x 250 mm, 5 microm) which eluted with methanol and water (content 0.02% phosphoric acid). The ratio of methanol increased from 20% to 80% during 20-50 min, and from 80% to 100% during 50-60 min, with detected wavelength 254 nm, flow rate at 1 mL x min(-1), column temperature 35 degrees C.


RESULTS
Six compounds were base-isolated, the linear ranges of swertiamarin, gentiopicroside, mangiferin, 4-swertianolin, 5-isoorientin, 1,8-drihydroxy-3-methoxy-xthanone were excellent.


CONCLUSIONS
The method was rapid and precise, and can be use for controlling medicinal materials quality.
Bao
Yi
Y
Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining 810001, China.
Ji
Wen-He
WH
Ma
Yu-Hua
YH
Ji
Lan-Ju
LJ
D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005960:Glucosides / Q000032:analysis*; D057888:Iridoid Glucosides; D039823:Iridoids / Q000032:analysis*; D047311:Luteolin / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D011714:Pyrans / Q000032:analysis; D011753:Pyrones / Q000032:analysis*; D011786:Quality Control; D015203:Reproducibility of Results; D029967:Swertia / Q000737:chemistry*; D044004:Xanthones / Q000032:analysis*
D005960:Glucosides; D057888:Iridoid Glucosides; D039823:Iridoids; D011714:Pyrans; D011753:Pyrones; D044004:Xanthones; C012997:gentiopicroside; C013592:mangiferin; C013270:swertiamarin; C057912:homoorientin; D047311:Luteolin