Cytotoxicity of diospyrin and its derivatives in relation to the generation of reactive oxygen species in tumour cells in vitro and in vivo.
BACKGROUND
Alkyl ethers (D2 and D7) synthesized from diospyrin (D1), a naphthoquinonoid isolated from Diospyros montana Roxb., were evaluated for cytotoxicity and capacity to generate reactive oxygen species (ROS) in tumour cells.


METHODS
The tumour inhibitory activity of the quinonoids was assessed in vivo against Ehrlich ascites carcinoma (EAC), while cytotoxicity was determined in vitro on EAC and MCF-7 cancer cells by MTT assay. ROS generated by quinonoids in MCF-7 cells was measured fluorimetrically.


RESULTS
The tumour inhibitory activity, cytotoxicity and ROS generation capacity of quinonoids were found to be D7 > D2 > D1.


CONCLUSIONS
Alkyl ethers of D1 were more cytotoxic against tumour cells in vitro as well as in vivo.
Hazra
B
B
Department of Pharmaceutical Technology, Jadavpur University, Calcutta, India.
Das Sarma
M
M
Kumar
B
B
Basu
S
S
Das
K
K
Pandey
B N
BN
Mishra
K P
KP
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy*; D045744:Cell Line, Tumor; D004987:Ethers / Q000494:pharmacology*; D006801:Humans; D015227:Lipid Peroxidation; D051379:Mice; D009285:Naphthoquinones / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D023041:Xenograft Model Antitumor Assays
D000972:Antineoplastic Agents, Phytogenic; D004987:Ethers; D009285:Naphthoquinones; D017382:Reactive Oxygen Species; C043546:diospyrin