Improved luciferase tagging system for Listeria monocytogenes allows real-time monitoring in vivo and in vitro.
An improved system for luciferase tagging Listeria monocytogenes was developed by constructing a highly active, constitutive promoter. This construct gave 100-fold-higher activity in broth than any native promoter tested and allowed for imaging of lux-tagged L. monocytogenes in food products, during murine infections, and in tumor targeting studies.
Riedel
Christian U
CU
Alimentary Pharmabiotic Centre and Department of Microbiology, University College Cork, Cork, Ireland.
Monk
Ian R
IR
Casey
Pat G
PG
Morrissey
David
D
O'Sullivan
Gerald C
GC
Tangney
Mark
M
Hill
Colin
C
Gahan
Cormac G M
CG
D000818:Animals; D001483:Base Sequence; D005513:Food Inspection / Q000379:methods*; D005516:Food Microbiology; D005822:Genetic Vectors / Q000235:genetics; D008089:Listeria monocytogenes / Q000378:metabolism*; D008156:Luciferases / Q000378:metabolism*; D049453:Luciferases, Bacterial; D051379:Mice; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA
D008156:Luciferases; D049453:Luciferases, Bacterial