Joint composite-rotation adiabatic-sweep isotope filtration.
Joint composite-rotation adiabatic-sweep isotope filters are derived by combining the composite-rotation [Stuart AC et al. (1999) J Am Chem Soc 121: 5346-5347] and adiabatic-sweep [Zwahlen C et al. (1997) J Am Chem Soc 119:6711-6721; Kupce E, Freeman R (1997) J Magn Reson 127:36-48] approaches. The joint isotope filters have improved broadband filtration performance, even for extreme values of the one-bond (1)H-(13)C scalar coupling constants in proteins and RNA molecules. An average Hamiltonian analysis is used to describe evolution of the heteronuclear scalar coupling interaction during the adiabatic sweeps within the isotope filter sequences. The new isotope filter elements permit improved selective detection of NMR resonance signals originating from (1)H spins attached to an unlabeled natural abundance component of a complex in which the other components are labeled with (13)C and (15)N isotopes.
Valentine
Elizabeth R
ER
Department of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, New York, NY 10032, USA.
Ferrage
Fabien
F
Massi
Francesca
F
Cowburn
David
D
Palmer
Arthur G
AG
D002247:Carbon Isotopes; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009587:Nitrogen Isotopes; D011506:Proteins / Q000737:chemistry; D012313:RNA / Q000737:chemistry; D015203:Reproducibility of Results
D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D011506:Proteins; D012313:RNA