title: Photodynamic diagnosis (PDD) using 5-aminolevulinic acid-supplemented cultures of human endometrial epithelial cells.
abstract: OBJECTIVE
  The studies were aimed at monitoring 5-aminolevulinic acid (5-ALA)-dependent accumulation of endogenous protoporphyrin IX (PpIX) in epithelial cells originating from normal endometrium or endometriotic foci, as related to steroid treatment.
  
  
  METHODS
  Epithelial cells were cultured in presence of estradiol-17 beta (E2) and progesterone (P) in concentrations typical for the follicular stage (E2 alone, 220 pg/ml) or the luteal stage (E2 100 pg/ml and P 2 ng/ml) or in presence of progesterone alone (2 ng/ml) for a period of 24, 48 or 72 h. Effect of 5-ALA concentration on the accumulation of PpIX was defined in the cells incubated with 2.0 mmol/l 5-ALA for 2 h. PpIX fluorescence was detected using a confocal microscope.
  
  
  RESULTS
  After hormonal stimulation, intensity of PpIX-specific fluorescence was only slightly increased in epithelial cells originating from normal endometrium. Cultures of epithelial cells from endometriosis foci showed higher concentration of PpIX than did the cells originating from normal endometrium. The highest peak of PpIX fluorescence was noted in epithelial endometriotic cells after 48h incubation with progesterone.
  
  
  CONCLUSIONS
  The data on PpIX accumulation in epithelial cells in the presence of estradiol-17 beta or progesterone may provide indications as to the menstrual cycle phase(s) in which photodynamic therapy for endometriosis should be performed. It is concluded that hormonal condition of female body must be taken into account for diagnosis and treatment of endometriosis.
authors.affiliation: Department of Radiobiology and Cell Biology, University of Medical Sciences, Poznań, Poland. doskon@amp.edu.pl
authors.forename: M
authors.initials: M
authors.lastname: Wołuń-Cholewa
authors.forename: W
authors.initials: W
authors.lastname: Warchoł
authors.forename: W
authors.initials: W
authors.lastname: Butowska
authors.forename: E
authors.initials: E
authors.lastname: Nowak-Markwitz
authors.forename: N
authors.initials: N
authors.lastname: Fischer
mesh_terms: D000622:Aminolevulinic Acid / Q000494:pharmacology*; D002478:Cells, Cultured; D004715:Endometriosis / Q000378:metabolism / Q000473:pathology; D004717:Endometrium / Q000166:cytology / Q000378:metabolism; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005453:Fluorescence; D006801:Humans; D018613:Microscopy, Confocal; D011374:Progesterone / Q000494:pharmacology*; D011524:Protoporphyrins / Q000737:chemistry / Q000378:metabolism*; D013997:Time Factors
chemical_list: D011524:Protoporphyrins; D011374:Progesterone; D004958:Estradiol; D000622:Aminolevulinic Acid; C028025:protoporphyrin IX