title: Erythrocyte susceptibility to oxidative stress and antioxidant status in patients with type 1 diabetes.
abstract: In this study, malondialdehyde (MDA) level as an index of erythrocyte susceptibility to oxidative stress and antioxidant defense system (glutathione level (GSH), glutathione peroxidase enzyme activity (GPx) in erythrocytes and ferric reducing ability of plasma (FRAP) as the total plasma antioxidant capacity were measured in 35 patients with type 1 diabetes and 28 age and sex-matched normal subjects. MDA level was significantly elevated in diabetic patients (650.9+/-144.3 nmol/g versus 476.5+/-138.5 nmol/g Hb, P<0.001). The level of MDA was positively correlated with duration of diabetes (r= 0.29, P<0.05) and HbA(1C) (r= 0.39, P<0.05) and negatively with FRAP (r= -0.3, P<0.05). The level of GSH and FRAP were lower in patients than controls (7.05+/-1.6 micromol/g versus 8.24+/-0.9 micromol/g Hb, and 389.05+/-82.3 micromol/l versus 520.4+/-124.1 micromol/l, respectively, P<0.001). GPx activity was not significantly different between the two groups. GSH and FRAP were negatively correlated with HbA(1C) (r= -0.334, P<0.01 and r= -0.5, P<0.01, respectively). In conclusion, there seems to be an increased susceptibility to oxidative stress and decreased antioxidant defense in patients with type 1 diabetes, which may be due to poor glycemic control.
authors.affiliation: Department of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Firoozrai
authors.forename: Mitra
authors.initials: M
authors.lastname: Nourbakhsh
authors.forename: Maryam
authors.initials: M
authors.lastname: Razzaghy-Azar
mesh_terms: D000293:Adolescent; D000975:Antioxidants / Q000032:analysis*; D016022:Case-Control Studies; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism / Q000473:pathology*; D005260:Female; D005978:Glutathione / Q000097:blood; D005979:Glutathione Peroxidase / Q000378:metabolism; D006441:Hemoglobin A / Q000032:analysis; D006801:Humans; D007501:Iron / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D018384:Oxidative Stress
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D006441:Hemoglobin A; D007501:Iron; D005979:Glutathione Peroxidase; D005978:Glutathione