title: QuiCk-IntraOperative Bio-Intact PTH assay at parathyroidectomy for secondary hyperparathyroidism.
abstract: BACKGROUND
  In uremic patients, metabolism of 1-84 parathyroid hormone (PTH) and fragments are delayed, and in these patients, the usefulness of intraoperative PTH assay may be problematic. We evaluated the usefulness of the QuiCk-IntraOperative Bio-Intact PTH (QPTH) assay for uremic patients with secondary hyperparathyroidism who required total parathyroidectomy (PTx) with forearm autograft. The purpose of our study was to recognize whether QPTH in uremic patients was useful to determine during operation whether complete PTx had been achieved.
  
  
  METHODS
  Forty-four patients who underwent initial PTx were enrolled in this study. Blood samples were drawn just after induction of general anesthesia (basal samples), immediately after removal of the last gland, and at 5, 10, 15, and 30 minutes, and at the first morning after PTx. The assay was performed immediately after sample collection. Reductions of PTH levels were evaluated and expressed in percentage of basal levels.
  
  
  RESULTS
  The mean PTH levels in 41 patients, excluding 3 in whom the PTH level did not drop significantly (>60 pg/ml), measured by QPTH at anesthesia, 0, 5, 10, 15, and 30 minutes were 734.3, 104.7, 58.8, 37.4, 27.0, 16.3 pg/ml, corresponding to 100%, 17.1%, 9.3%, 5.8%, 4.1%, 2.4% of the preexcision values, respectively. If the cutoff value was defined as 10.8% at 10 minutes, the sensitivity was 100% and specificity 90%. When the QPTH level dropped to under 10.8% at 10 minutes, we could consider that all glands were removed.
  
  
  CONCLUSIONS
  QPTH in uremic patients is very useful to determine whether complete PTx is achieved during operation.
authors.affiliation: Department of Transplant and Endocrine Surgery, Nagoya Daini Red Cross Hospital, Nagoya, Japan. matsuoka@nagoya2.jrc.or.jp
authors.forename: Susumu
authors.initials: S
authors.lastname: Matsuoka
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Tominaga
authors.forename: Tetsuhiko
authors.initials: T
authors.lastname: Sato
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Uno
authors.forename: Norihiko
authors.initials: N
authors.lastname: Goto
authors.forename: Akio
authors.initials: A
authors.lastname: Katayama
authors.forename: Kazuharu
authors.initials: K
authors.lastname: Uchida
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takami
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D006962:Hyperparathyroidism, Secondary / Q000601:surgery*; D007118:Immunoassay; D008297:Male; D008875:Middle Aged; D010280:Parathyroid Glands / Q000378:metabolism* / Q000601:surgery*; D010281:Parathyroid Hormone / Q000097:blood*; D016105:Parathyroidectomy; D012372:ROC Curve; D012680:Sensitivity and Specificity
chemical_list: D010281:Parathyroid Hormone