Effects of alpha-linolenic acid on colonic secretion in rats with experimental colitis.
BACKGROUND
Few studies have specifically examined the effects of n-3 polyunsaturated fatty acids (PUFAs) on intestinal water and ion secretion in ulcerative colitis (UC). The aim of this study was to examine the contribution of prostaglandins (PGs) and leukotrienes (LTs) to mucosal secretion in intestines with UC and to evaluate the effect of dietary n-3 PUFAs on diarrhea in UC.


METHODS
We measured the short-circuit current (Isc), using the Ussing chamber method, and fatty acid composition in the colonic mucosa of rats with dextran sulfate sodium (DSS)-induced experimental colitis. The DSS-treated rats were fed either a perilla oil-enriched diet (perilla group) or a soybean oil-enriched diet (soybean group); a control group did not undergo DSS administration.


RESULTS
The bradykinin-stimulated DeltaIsc in the soybean and perilla groups was significantly higher than that in the control group. The mucosal level of arachidonic acid in the perilla group was significantly lower than that in the soybean group. The mucosal levels of alpha-linolenic acid and EPA in the perilla group were significantly higher than those in the soybean group. The bradykinin-stimulated DeltaIsc was significantly suppressed after pretreatment with indomethacin in both the soybean and perilla groups, and was also significantly reduced in both groups after pretreatment with AA861. The suppression of bradykinin-stimulated DeltaIsc by the addition of AA861 was significantly higher in the perilla group than in the soybean group.


CONCLUSIONS
Our results suggest that supplementation with alpha-linolenic acid, in combination with a lipoxygenase inhibitor, could suppress the increase in Cl- secretion in patients with UC.
Shimizu
Toshiaki
T
Department of Pediatrics, Juntendo University School of Medicine, 2-1-1 Hongo, Tokyo, Japan.
Kitamura
Tomohiro
T
Suzuki
Mitsuyoshi
M
Fujii
Tohru
T
Shoji
Hiromich
H
Tanaka
Kyoko
K
Igarashi
Jun
J
D000818:Animals; D003093:Colitis, Ulcerative / Q000378:metabolism*; D003106:Colon / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D015289:Leukotrienes / Q000502:physiology*; D008297:Male; D011453:Prostaglandins / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D017962:alpha-Linolenic Acid / Q000502:physiology*
D015289:Leukotrienes; D011453:Prostaglandins; D017962:alpha-Linolenic Acid