title: [Construction of Sjcb2 DNA vaccine and its expression in HeLa cells].
abstract: A recombinant plasmid containing cathepsin B endopeptidase of Schistosoma japonicum (Sjcb2) was constructed, indentified by PCR, restrictive enzyme, digestion and DNA sequencing, and expressed into mammalian cells. Immunochemistry examination showed that the Sjcb2 gene can be expressed in the eukaryotic system, providing a basis for the development of schistosome DNA vaccine.
authors.affiliation: Institute of Pathogenic Biology, Nanhua University, Hengyang 421001, China.
authors.forename: Yong-xuan
authors.initials: YX
authors.lastname: Hu
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Xiao
authors.forename: Jia-fang
authors.initials: JF
authors.lastname: Huang
authors.forename: Qiu-lin
authors.initials: QL
authors.lastname: Yang
mesh_terms: D000818:Animals; D002401:Cathepsin B / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods; D004274:DNA, Recombinant; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D015247:Deoxyribonuclease HindIII / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D006367:HeLa Cells; D015801:Helminth Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D010957:Plasmids / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012550:Schistosoma mansoni / Q000201:enzymology / Q000235:genetics / Q000276:immunology; D012555:Schistosomiasis mansoni / Q000276:immunology; D017422:Sequence Analysis, DNA; D014162:Transfection; D019444:Vaccines, DNA / Q000235:genetics* / Q000276:immunology
chemical_list: D004274:DNA, Recombinant; D015801:Helminth Proteins; D019444:Vaccines, DNA; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; C466432:CB2 protein, Schistosoma mansoni; D002401:Cathepsin B