[A new molecular method to authenticate radix trichosanthis as well as its adulterants and substitutes].
OBJECTIVE
To explore a new molecular method to authenticate Radix Trichosanthis.


METHODS
Three 20 mer primers based on the ITS sequence was designed. The PCR reaction system was optimized and applied to nineteen different sources of Radix Trichosanthis and nine adulterants and substitutes.


RESULTS
Polymorphic map of Radix Trichosanthis and its adulterants was obtained from primer TKS1-64. 560 bp and 960 bp bands were authentic markers for Radix Trichosanthis.


CONCLUSIONS
Primer TKS1-64F possesses the advantages of good stability and reproducibility. This new method is named as anchored primer amplification polymorphism DNA(APAPD). It was a potential method to used in molecular identification of other meteria medica.
Cui
Guang-Hong
GH
Institute of Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, China.
Huang
Lu-Qi
LQ
Wang
Min
M
D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics; D021903:DNA, Ribosomal Spacer; D004340:Drug Contamination / Q000517:prevention & control; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods; D015203:Reproducibility of Results; D029778:Trichosanthes / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D015978:Trichosanthin / Q000235:genetics* / Q000302:isolation & purification
D017931:DNA Primers; D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer; D015978:Trichosanthin