title: Exceptionally high representation of Burkholderia cepacia among B. cepacia complex isolates recovered from the major Portuguese cystic fibrosis center.
abstract: Burkholderia cepacia, a species found infrequently in cystic fibrosis (CF), was isolated from 85% of patients infected with bacteria of the B. cepacia complex that visited the major Portuguese CF center, in Lisbon, during 2003 to 2005. A detailed molecular analysis revealed that this was mainly due to two B. cepacia clones. These clones were indistinguishable from two strains isolated from intrinsically contaminated nonsterile saline solutions for nasal application, detected during routine market surveillance by the Portuguese Medicines and Health Products Authority.
authors.affiliation: IBB-Institute for Biological and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.
authors.forename: Mónica V
authors.initials: MV
authors.lastname: Cunha
authors.forename: Ana
authors.initials: A
authors.lastname: Pinto-de-Oliveira
authors.forename: Luís
authors.initials: L
authors.lastname: Meirinhos-Soares
authors.forename: Maria José
authors.initials: MJ
authors.lastname: Salgado
authors.forename: José
authors.initials: J
authors.lastname: Melo-Cristino
authors.forename: Susana
authors.initials: S
authors.lastname: Correia
authors.forename: Celeste
authors.initials: C
authors.lastname: Barreto
authors.forename: Isabel
authors.initials: I
authors.lastname: Sá-Correia
mesh_terms: D019121:Burkholderia Infections / Q000150:complications / Q000453:epidemiology* / Q000382:microbiology*; D016956:Burkholderia cepacia / Q000302:isolation & purification*; D042602:Burkholderia cepacia complex / Q000302:isolation & purification*; D003550:Cystic Fibrosis / Q000150:complications* / Q000453:epidemiology*; D006801:Humans; D011159:Population Surveillance; D011174:Portugal / Q000453:epidemiology; D013997:Time Factors