[Expression of bcl-2 gene in EBV-transformed human gastric epithelial cell line GES-1].
OBJECTIVE
To detect bcl-2 gene expression in Epstein-Barr virus (EBV)-infected human gastric epithelial cell line GES-1 for understanding the role of bcl-2 gene in the carcinogenesis of EBV-associated gastric carcinoma.


METHODS
Akata 1061 cells producing recombined EBV carrying neomycin resistance gene (NEOr) was used to mediate the EBV infection of human gastric epithelial cell line GES-1 via close contact, with the empty plasmid pcDNA3-transfected GES-1 cells via lipofectamine method as a control. The EBV-infected and pcDNA3-transfected cells were cloned by limited dilution and the positive clones selected with G418. Immunocytochemical staining was performed to detect the expressions of EBNA1 and Bcl-2 protein.


RESULTS
Bcl-2 protein expression was detected in EBV-infected cells but not in the control cells.


CONCLUSIONS
EBV infection can increase Bcl-2 expression in gastric epithelial cells, and such cell transformation effect of EBV is related to the overexpression of bcl-2 gene.
Zhu
Li-Hua
LH
Department of Biology, North China Coal Medical College, Tangshan 063000, China. zhulihua1972@163.com
Zhou
Tian-Ji
TJ
Shi
Guo-You
GY
Zhang
Guang-Ling
GL
Li
Shu-Ying
SY
D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism* / Q000821:virology; D004854:Herpesvirus 4, Human; D006801:Humans; D007150:Immunohistochemistry; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis*; D013270:Stomach / Q000166:cytology
D019253:Proto-Oncogene Proteins c-bcl-2