Efficient surface patterning of oligonucleotides inside a glass capillary through oxime bond formation.
The efficient surface patterning of oligonucleotides was accomplished onto the inner wall of fused-silica capillary tubes as well as on the surface of glass slides through oxime bond formation. The robustness of the method was demonstrated by achieving the surface immobilization of up to three different oligonucleotide sequences inside the same capillary tube. The method involves the preparation of surfaces grafted with reactive aminooxy functionalities masked with the photocleavable protecting group, 2-(2-nitrophenyl) propyloxycarbonyl group (NPPOC). Briefly, NPPOC-aminooxy silane 1 was prepared and used to silanize the glass surfaces. The NPPOC group was cleaved under brief irradiation to unmask the reactive aminooxy group on surfaces. These reactive aminooxy groups were allowed to react with aldehyde-containing oligonucleotides to achieve an efficient surface immobilization. The advantage associated with the present approach is that it combines the high-coupling efficiency of oxime bond formation with the convenience associated with the use of photolabile groups. The present strategy thus offers an alternative approach for the immobilization of biomolecules in the microchannels of "labs on a chip" devices.
Dendane
Nabil
N
Département de Chimie Moléculaire - UMR CNRS 5250, ICMG FR2607, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France.
Hoang
Antoine
A
Guillard
Ludovic
L
Defrancq
Eric
E
Vinet
Françoise
F
Dumy
Pascal
P
D002219:Carbamates / Q000138:chemical synthesis / Q000737:chemistry*; D005898:Glass / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis; D009841:Oligonucleotides / Q000737:chemistry*; D010091:Oximes / Q000737:chemistry*; D012821:Silanes / Q000138:chemical synthesis / Q000737:chemistry*; D013499:Surface Properties
C522251:11-(triethoxysilyl)-1-(N-(2-(2-nitrophenyl)propoxycarbonyl)aminooxy)undecane; D002219:Carbamates; D009841:Oligonucleotides; D010091:Oximes; D012821:Silanes