title: Bell's expression and the generalized Garg form for forced dissociation of a biomolecular complex.
abstract: The dissociation of a biomolecular complex under the action of constant force, constant loading rate, and periodic force is studied theoretically. We show that the celebrated Bell expression provides a good approximation for the bond dissociation rate when F/F(c)<<1, where F(c) is the maxima slope of the binding potential along the reaction coordinate. When 1-F/F(c) <<1 the dissociation rate is better described by a generalized Garg form in which the potential derivative is expanded near F(c). We also show that a constant-force experiment is suitable for extracting the activation energy of the bond, a constant loading experiment is suitable to extract F(c), while time-periodic force can be applied to extract both bond dissociation rates at zero force and F(c).
authors.affiliation: Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan 106, Republic of China.
authors.forename: Han-Jou
authors.initials: HJ
authors.lastname: Lin
authors.forename: Hsuan-Yi
authors.initials: HY
authors.lastname: Chen
authors.forename: Yu-Jane
authors.initials: YJ
authors.lastname: Sheng
authors.forename: Heng-Kwong
authors.initials: HK
authors.lastname: Tsao
mesh_terms: D003198:Computer Simulation; D046911:Macromolecular Substances / Q000737:chemistry*; D008956:Models, Chemical; D011485:Protein Binding; D013816:Thermodynamics
chemical_list: D046911:Macromolecular Substances