title: Preliminary analysis of cold stress responsive proteins in Mesocestoides corti larvae.
abstract: Many parasites undergo sudden changes in environmental conditions at some stage during their life cycle. The molecular response to this variation is characterised by a rapid transcriptional activation of a specific set of genes coding for proteins generically known as stress proteins. They appear to be also involved in various biological processes including cell proliferation and differentiation. The platyhelminth parasite, Mesocestoides corti (Cestoda) presents important properties as a model organism. Under stress conditions, key molecules involved in metabolic pathways as well as in the growth and differentiation of the parasite can be identified. 2D protein expression profile of tetrathyridia of M. corti, submitted to nutritional starvation and cold stress is described, as well as the recovery pattern. A set of specifically expressed proteins was observed in each experimental condition. Quantitative and qualitative differences and stress recovery pattern are also reported. This work makes evident the high plasticity and resistance to extreme environmental conditions of these parasites at the molecular level.
authors.affiliation: Biochemistry Section, Department of Cellular and Molecular Biology, Faculty of Sciences, University of the Republic of Uruguay, Iguá 4225, Montevideo, Uruguay.
authors.forename: Lucía
authors.initials: L
authors.lastname: Canclini
authors.forename: Adriana
authors.initials: A
authors.lastname: Esteves
mesh_terms: D000818:Animals; D003080:Cold Temperature; D015180:Electrophoresis, Gel, Two-Dimensional; D018507:Gene Expression Regulation, Developmental; D006360:Heat-Shock Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D015801:Helminth Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D007526:Isoelectric Point; D007814:Larva / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D008297:Male; D008645:Mesocestoides / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D040901:Proteomics; D016622:Silver Staining
chemical_list: D006360:Heat-Shock Proteins; D015801:Helminth Proteins