[Construction of Sjcb2 DNA vaccine and its expression in HeLa cells].
A recombinant plasmid containing cathepsin B endopeptidase of Schistosoma japonicum (Sjcb2) was constructed, indentified by PCR, restrictive enzyme, digestion and DNA sequencing, and expressed into mammalian cells. Immunochemistry examination showed that the Sjcb2 gene can be expressed in the eukaryotic system, providing a basis for the development of schistosome DNA vaccine.
Hu
Yong-xuan
YX
Institute of Pathogenic Biology, Nanhua University, Hengyang 421001, China.
Xiao
Jian-hua
JH
Huang
Jia-fang
JF
Yang
Qiu-lin
QL
D000818:Animals; D002401:Cathepsin B / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods; D004274:DNA, Recombinant; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D015247:Deoxyribonuclease HindIII / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D006367:HeLa Cells; D015801:Helminth Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D010957:Plasmids / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012550:Schistosoma mansoni / Q000201:enzymology / Q000235:genetics / Q000276:immunology; D012555:Schistosomiasis mansoni / Q000276:immunology; D017422:Sequence Analysis, DNA; D014162:Transfection; D019444:Vaccines, DNA / Q000235:genetics* / Q000276:immunology
D004274:DNA, Recombinant; D015801:Helminth Proteins; D019444:Vaccines, DNA; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; C466432:CB2 protein, Schistosoma mansoni; D002401:Cathepsin B