title: Empirical model functions to calculate hematocrit-dependent optical properties of human blood.
abstract: The absorption coefficient, scattering coefficient, and effective scattering phase function of human red blood cells (RBCs) in saline solution were determined for eight different hematocrits (Hcts) between 0.84% and 42.1% in the wavelength range of 250-1100 nm using integrating sphere measurements and inverse Monte Carlo simulation. To allow for biological variability, averaged optical parameters were determined under flow conditions for ten different human blood samples. Based on this standard blood, empirical model functions are presented for the calculation of Hct-dependent optical properties for the RBCs. Changes in the optical properties when saline solution is replaced by blood plasma as the suspension medium were also investigated.
authors.affiliation: Institut für Medizinische Physik and Lasermedizin, Campus Benjamin Franklin, Charité-Universitätsmedizin, Berlin, Germany. martina.meinke@charite.de
authors.forename: Martina
authors.initials: M
authors.lastname: Meinke
authors.forename: Gerhard
authors.initials: G
authors.lastname: Müller
authors.forename: Jürgen
authors.initials: J
authors.lastname: Helfmann
authors.forename: Moritz
authors.initials: M
authors.lastname: Friebel
mesh_terms: D000465:Algorithms; D001790:Blood Physiological Phenomena; D003198:Computer Simulation; D006400:Hematocrit; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D008027:Light; D008955:Models, Cardiovascular; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D011829:Radiation Dosage; D011874:Radiometry / Q000379:methods*; D012542:Scattering, Radiation