title: A versatile multimode microscope to probe and manipulate nanoparticles and biomolecules.
abstract: We describe a flexible, multifaceted optical setup that allows quantitative measurement and manipulation of biomolecules and nanoparticles in biomimetic and cellular systems. We have implemented integrated biophotonics techniques (i.e. differential interference contrast, wide-field fluorescence, prism- and objective-based total internal reflection excitation, single particle tracking, fluorescence correlation spectroscopy and dynamic holographic optical trapping) on a single platform. The adaptability of this versatile, custom-designed system allows us to simultaneously monitor cell morphology, while measuring lateral diffusion of biomolecules or controlling their cellular location or interaction partners.
authors.affiliation: Department of Chemistry, Pennsylvania State University, University Park PA 16802, USA.
authors.forename: M
authors.initials: M
authors.lastname: Kyoung
authors.forename: K
authors.initials: K
authors.lastname: Karunwi
authors.forename: E D
authors.initials: ED
authors.lastname: Sheets
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D004867:Equipment Design; D006696:Holography / Q000295:instrumentation; D051379:Mice; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D053758:Nanoparticles / Q000648:ultrastructure*; D055095:Optics and Photonics / Q000295:instrumentation; D051381:Rats; D013050:Spectrometry, Fluorescence / Q000295:instrumentation
chemical_list: D000199:Actins