Simultaneous determination of myo-inositol and scyllo-inositol by MEKC as a rapid monitoring tool for inositol levels.
A simple and rapid MEKC method was developed for the simultaneous determination of myo-inositol, scyllo-inositol, and glucose. Prior to electrophoretic separation, the nonfluorescent inositols and glucose were derivatized by N-methylisatoic anhydride at 25 degrees C for 10 min so that they could be detected by a fluorescence detector during separation. The good separation with high efficiency by MEKC was achieved in 13 min with a glycine buffer containing SDS and PEG 4000. Several parameters affecting the separation were studied, including the pH of BGE, the concentrations of glycine, SDS, and PEG 4000, and the applied voltage. Using glycerol as an internal standard, the linear ranges of the method for myo-inositol, scyllo-inositol, and glucose were 0.03-10, 0.01-5, and 0.05-20 mM; the concentration LODs of myo-inositol, scyllo-inositol, and glucose were 0.020, 0.0078, and 0.026 mM, respectively. The method was applied to analyze extracellular myo-inositol and glucose in the microdialysates from rat brain cortex of ischemia animal model and intracellular myo-inositol and scyllo-inositol in the rat brain extract.
Tzeng
Huey-Fen
HF
Department of Applied Chemistry, National Chi Nan University, Puli, Nantou, Taiwan. hftzeng@ncnu.edu.tw
Chen
Jin-Yi
JY
Huang
Shih-Wei
SW
Wang
Yu-Jing
YJ
Yang
Chung-Shi
CS
D000812:Anhydrides / Q000737:chemistry; D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D005947:Glucose / Q000031:analogs & derivatives / Q000737:chemistry; D005998:Glycine; D006863:Hydrogen-Ion Concentration; D007294:Inositol / Q000032:analysis* / Q000737:chemistry; D008297:Male; D011092:Polyethylene Glycols; D051381:Rats; D017207:Rats, Sprague-Dawley; D012967:Sodium Dodecyl Sulfate; D013237:Stereoisomerism; D062367:ortho-Aminobenzoates / Q000737:chemistry
D000812:Anhydrides; D062367:ortho-Aminobenzoates; C077537:N-methylisatoic anhydride; C009217:scyllitol; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols; D007294:Inositol; D005947:Glucose; D005998:Glycine