Neurovascular coupling is not mediated by potassium siphoning from glial cells.
Neuronal activity evokes localized changes in blood flow, a response termed neurovascular coupling. One widely recognized hypothesis of neurovascular coupling holds that glial cell depolarization evoked by neuronal activity leads to the release of K+ onto blood vessels (K+ siphoning) and to vessel relaxation. We now present two direct tests of this glial cell-K+ siphoning hypothesis of neurovascular coupling. Potassium efflux was evoked from glial cells in the rat retina by applying depolarizing current pulses to individual cells. Glial depolarizations as large as 100 mV produced no change in the diameter of adjacent arterioles. We also monitored light-evoked vascular responses in Kir4.1 knock-out mice, where functional Kir K+ channels are absent from retinal glial cells. The magnitude of light-evoked vasodilations was identical in Kir4.1 knock-out and wild-type animals. Contrary to the hypothesis, the results demonstrate that glial K+ siphoning in the retina does not contribute significantly to neurovascular coupling.
Metea
Monica R
MR
Department of Neuroscience, University of Minnesota, Minneapolis, Minnesota 55455, USA.
Kofuji
Paulo
P
Newman
Eric A
EA
D000818:Animals; D001160:Arterioles / Q000502:physiology; D004594:Electrophysiology; D066298:In Vitro Techniques; D008027:Light; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009424:Nervous System Physiological Phenomena; D009457:Neuroglia / Q000378:metabolism / Q000502:physiology*; D011188:Potassium / Q000378:metabolism*; D024661:Potassium Channels, Inwardly Rectifying / Q000172:deficiency; D051381:Rats; D020318:Rats, Long-Evans; D012039:Regional Blood Flow / Q000502:physiology; D012160:Retina / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D012171:Retinal Vessels / Q000502:physiology*; D014664:Vasodilation / Q000502:physiology / Q000528:radiation effects; D014666:Vasomotor System / Q000528:radiation effects; D000098854:Kcnj10 Channel
D000098854:Kcnj10 Channel; D024661:Potassium Channels, Inwardly Rectifying; D011188:Potassium