Profile of acute immune response in Chinese amphioxus upon Staphylococcus aureus and Vibrio parahaemolyticus infection.
Amphioxus is traditionally considered as the living invertebrate most closely related to vertebrate. However, no systematic study was performed about how the amphioxus defends against the microbial invasion. Here we reported a profile of gene transcription after Staphylococcus aureus (S.c) and Vibrio parahaemolyticus (V.p) challenged by suppression subtractive hybridization (SSH). When compared with mammals, amphioxus has the same acute immune defense genes (lectins, metalloproteinase, lysozymes and antimicrobial peptide, etc.) as well as a similar pattern and level of temporal gene expression. In contrast, amphioxus was demonstrated to have some novel acute immune response genes in response to the microbial challenge, such as apextrin and dermatopontin, which have a 3500-fold and 900-fold induction after the V.p infection, respectively, suggesting new functions in early immune system for these two genes. Our results reported for the first time a profile of primitive immune system defense against infection in protochordate.
Huang
Gonghua
G
State Key Laboratory of Biocontrol, College of Life Sciences, Sun Yat-sen University, Guangzhou 510275, People's Republic of China.
Liu
Hui
H
Han
Yan
Y
Fan
Lifei
L
Zhang
Qinfen
Q
Liu
Jianzhong
J
Yu
Xuesong
X
Zhang
Lichun
L
Chen
Shangwu
S
Dong
Meiling
M
Wang
Lei
L
Xu
Anlong
A
D000208:Acute Disease; D000818:Animals; D002816:Chordata, Nonvertebrate / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology; D020869:Gene Expression Profiling; D013203:Staphylococcal Infections / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology*; D014735:Vibrio Infections / Q000276:immunology*; D014736:Vibrio parahaemolyticus / Q000276:immunology*