Early and specific gene expression triggered by rice resistance gene Pi33 in response to infection by ACE1 avirulent blast fungus.
* Our view of genes involved in rice disease resistance is far from complete. Here we used a gene-for-gene relationship corresponding to the interaction between atypical avirulence gene ACE1 from Magnaporthe grisea and rice resistance gene Pi33 to better characterize early rice defence responses induced during such interaction. * Rice genes differentially expressed during early stages of Pi33/ACE1 interaction were identified using DNA chip-based differential hybridization and QRT-PCR survey of the expression of known and putative regulators of disease resistance. * One hundred genes were identified as induced or repressed during rice defence response, 80% of which are novel, including resistance gene analogues. Pi33/ACE1 interaction also triggered the up-regulation of classical PR defence genes and a massive down-regulation of chlorophyll a/b binding genes. Most of these differentially expressed genes were induced or repressed earlier in Pi33/ACE1 interaction than in the gene-for-gene interaction involving Nipponbare resistant cultivar. * Besides demonstrating that an ACE1/Pi33 interaction induced classical and specific expression patterns, this work provides a list of new genes likely to be involved in rice disease resistance.
Vergne
E
E
UMR BGPI INRA/CIRAD/AgroM, Campus International de Baillarguet, T41/K 34398 Montpellier, France.
Ballini
E
E
UMR BGPI INRA/CIRAD/AgroM, Campus International de Baillarguet, T41/K 34398 Montpellier, France.
Marques
S
S
UMR BGPI INRA/CIRAD/AgroM, Campus International de Baillarguet, T41/K 34398 Montpellier, France.
Sidi Mammar
B
B
UMR BGPI INRA/CIRAD/AgroM, Campus International de Baillarguet, T41/K 34398 Montpellier, France.
Droc
G
G
UMR PIA CIRAD TA40/03 Avenue Agropolis 34398 Montpellier Cedex 5, France.
Gaillard
S
S
UMR PIA CIRAD TA40/03 Avenue Agropolis 34398 Montpellier Cedex 5, France.
Bourot
S
S
Bayer BioScience NV, Technologiepark 38, B-9052 Zwijnaarde, Belgium.
DeRose
R
R
Bayer BioScience NV, Technologiepark 38, B-9052 Zwijnaarde, Belgium.
Tharreau
D
D
UMR BGPI INRA/CIRAD/AgroM, Campus International de Baillarguet, T41/K 34398 Montpellier, France.
Nottéghem
J-L
JL
UMR BGPI INRA/CIRAD/AgroM, Campus International de Baillarguet, T41/K 34398 Montpellier, France.
Lebrun
M-H
MH
UMR 2579 CNRS, BayerCropscience, 14-20 Rue Pierre Baizet BP 9163, 69263 Lyon Cedex 09, France.
Morel
J-B
JB
UMR BGPI INRA/CIRAD/AgroM, Campus International de Baillarguet, T41/K 34398 Montpellier, France.
D015536:Down-Regulation; D018506:Gene Expression Regulation, Plant; D005800:Genes, Fungal; D017343:Genes, Plant; D020082:Magnaporthe / Q000235:genetics / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation