title: Immunization of nonautoimmune mice with DNA binding domains of the largest subunit of RNA polymerase I results in production of anti-dsDNA and anti-Sm/RNP antibodies.
abstract: Antibodies against the N-terminal (NT) but not the basic domain (BD), DNA binding regions of the largest subunit (S1) of RNA polymerase I (RNAPI) were detected in the sera of MRL-lpr/lpr lupus mice. Antibodies against both RNAPI(S1)-NT and -BD, as well as other systemic lupus erythematosus (SLE) autoantigens (La, ribosomal P proteins and Sm/RNP) were produced by rabbits immunized with anti-DNA antibodies that had been affinity purified from SLE patients. Immunization of nonautoimmune mice (Balb/c) with RNAPI(S1)-NT, RNAPI(S1)-BD, or La in the form of GST fusion proteins, induced production of anti-double-stranded (ds) DNA and anti-Sm/RNP. GST-P1 did not induce an anti-dsDNA response in these mice. These results demonstrate that RNAPI(S1)-NT, RNAPI(S1)-BD and La can participate in an anti-autoantigen/anti-DNA antibody loop during an SLE-like autoimmune response.
authors.affiliation: Department of Molecular Biosciences, University of Kansas. Lawrence, KS 66045, USA.
authors.forename: Sandra A
authors.initials: SA
authors.lastname: Sciascia
authors.forename: Kristina
authors.initials: K
authors.lastname: Robson
authors.forename: Liangjin
authors.initials: L
authors.lastname: Zhu
authors.forename: Michael
authors.initials: M
authors.lastname: Garland
authors.forename: Shannon
authors.initials: S
authors.lastname: Grabosch
authors.forename: Joseph
authors.initials: J
authors.lastname: Kelamis
authors.forename: Will
authors.initials: W
authors.lastname: Messamore
authors.forename: Todd
authors.initials: T
authors.lastname: Bradley
authors.forename: Allison
authors.initials: A
authors.lastname: Sourk
authors.forename: Lindsey
authors.initials: L
authors.lastname: Westberg
authors.forename: Waco
authors.initials: W
authors.lastname: Goodnight
authors.forename: Krystina
authors.initials: K
authors.lastname: Tongson
authors.forename: Naomi
authors.initials: N
authors.lastname: Holloway
authors.forename: Zabi
authors.initials: Z
authors.lastname: Wardak
authors.forename: Michelle
authors.initials: M
authors.lastname: Sudyka
authors.forename: Shriti
authors.initials: S
authors.lastname: Masrani
authors.forename: Sanket
authors.initials: S
authors.lastname: Chintalapati
authors.forename: Manuel
authors.initials: M
authors.lastname: Cagaanan
authors.forename: John C
authors.initials: JC
authors.lastname: Brown
authors.forename: Dean A
authors.initials: DA
authors.lastname: Stetler
mesh_terms: D000818:Animals; D000974:Antibodies, Antinuclear / Q000096:biosynthesis* / Q000276:immunology; D001324:Autoantigens / Q000096:biosynthesis / Q000276:immunology*; D015551:Autoimmunity / Q000276:immunology; D005260:Female; D007114:Immunization; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D019463:Mice, Inbred MRL lpr; D017434:Protein Structure, Tertiary; D012318:RNA Polymerase I / Q000276:immunology*; D011817:Rabbits; D051381:Rats; D012261:Ribonucleoproteins / Q000096:biosynthesis / Q000276:immunology; D017411:Ribonucleoproteins, Small Nuclear / Q000096:biosynthesis / Q000276:immunology*; D055517:snRNP Core Proteins; D000097585:SS-B Antigen
chemical_list: D000974:Antibodies, Antinuclear; D001324:Autoantigens; D012318:RNA Polymerase I; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear; D055517:snRNP Core Proteins; D000097585:SS-B Antigen