title: Pseudomonas fluorescens SBW25 biofilm and planktonic cells have differentiable Raman spectral profiles.
abstract: Biofilms, and other bacterial aggregations, are of significance in both environmental microbiology and in plant and human pathogenesis. Comparative single-cell Raman spectral analysis can differentiate between planktonic bacteria and those recovered from biofilms and appears to offer a new means by which to investigate bacterial cell physiology, metabolic status, and stress under different environmental conditions.
authors.affiliation: Molecular Microbiology Ecology Section, CEH-Oxford, Mansfield Road, Oxford, OX1 3SR, UK.
authors.forename: Wei E
authors.initials: WE
authors.lastname: Huang
authors.forename: Susanne
authors.initials: S
authors.lastname: Ude
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Spiers
mesh_terms: D001557:Benzenesulfonates; D018441:Biofilms; D016002:Discriminant Analysis; D004783:Environmental Microbiology; D005456:Fluorescent Dyes; D010933:Plankton; D025341:Principal Component Analysis; D011551:Pseudomonas fluorescens / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification / Q000502:physiology; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D001557:Benzenesulfonates; D005456:Fluorescent Dyes; C007061:C.I. Fluorescent Brightening Agent 28