title: Detection of respiratory pathogens in porcine lung tissue and lavage fluid.
abstract: The objective of this study was to compare the detection rate of bacterial agents in bronchoalveolar lavage fluid (BALF), taken without visual control, to that in affected lung tissue obtained from the same pig at necropsy. BALF and affected lung tissue were examined for Mycoplasma hyopneumoniae using PCR, and standard cultural methods were used for Actinobacillus pleuropneumoniae, Bordetella bronchiseptica, Haemophilus parasuis, Pasteurella multocida and Streptococcus suis. All pigs with a history of respiratory symptoms were submitted as live animals for routine diagnostic examination. In each animal the site of lavage, marked by injecting methylene blue, differed from the site of pneumonic lesions. M. hyopneumoniae was detected more frequently in lung tissue than in BALF in cases with moderate or severe lung lesions. The detection rates of M. hyopneumoniae were higher in the BALF of pigs with mild lesions. Cultural examination of BALF was at least as satisfactory as affected lung tissue for detecting B. bronchiseptica, H. parasuis and P. multocida.
authors.affiliation: Field Station for Epidemiology, University of Veterinary Medicine Hannover, Foundation, Bakum 49456, Germany.
authors.forename: Lars
authors.initials: L
authors.lastname: Moorkamp
authors.forename: Heiko
authors.initials: H
authors.lastname: Nathues
authors.forename: Joachim
authors.initials: J
authors.lastname: Spergser
authors.forename: Regina
authors.initials: R
authors.lastname: Tegeler
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Grosse Beilage
mesh_terms: D000818:Animals; D001419:Bacteria / Q000302:isolation & purification*; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology*; D008168:Lung / Q000382:microbiology*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*