Requirement of Rac1 distinguishes follicular from interfollicular epithelial stem cells.
Epithelial stem cells in the bulge region within the hair follicle maintain the cyclic hair growth, but whether these stem cells also contribute to the epidermal renewal remains unclear. Here, we observed that the conditional deletion of the Rac1 gene in the mouse skin, including the potential follicular and epidermal stem cell compartments, results in alopecia owing to defective hair development. Surprisingly, mice lacking the expression of this Rho GTPase do not display major alterations in the interfollicular skin. Furthermore, Rac1 excision from primary epithelial keratinocytes results in the inability to reconstitute hair follicles and sebaceous glands when grafted onto mice, but epithelial cells lacking Rac1 can nonetheless form a healthy epidermis. Together, these findings support the emerging view that the epidermis and the hair follicles are maintained by different epithelial stem cells, and provide evidence that the requirement for Rac1 function can distinguish these distinct stem cells populations.
Castilho
R M
RM
Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892-4340, USA.
Squarize
C H
CH
Patel
V
V
Millar
S E
SE
Zheng
Y
Y
Molinolo
A
A
Gutkind
J S
JS
D000818:Animals; D002465:Cell Movement / Q000235:genetics; D000078404:Epidermal Cells; D004817:Epidermis / Q000201:enzymology / Q000502:physiology*; D004847:Epithelial Cells / Q000201:enzymology / Q000502:physiology; D017353:Gene Deletion; D018859:Hair Follicle / Q000002:abnormalities / Q000166:cytology* / Q000254:growth & development; D015603:Keratinocytes / Q000201:enzymology / Q000502:physiology*; D051379:Mice; D008817:Mice, Mutant Strains; D009479:Neuropeptides / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D012038:Regeneration / Q000235:genetics; D013234:Stem Cells / Q000201:enzymology / Q000502:physiology*; D020744:rac GTP-Binding Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D020830:rac1 GTP-Binding Protein
D009479:Neuropeptides; C484602:Rac1 protein, mouse; D020744:rac GTP-Binding Proteins; D020830:rac1 GTP-Binding Protein