title: In vivo mutagenesis using EZ-Tn5.
abstract: Epicentre Biotechnologies has developed a suite of transposon-based tools for use in modern bacterial genetics. This chapter highlights the EZ-Tn5 Transposome system and focuses on in vivo mutagenesis and subsequent rescue cloning. Many other applications and variations have been described and are available through Epicentre's website at http://www.epibio.com/.
authors.affiliation: School of Biology, Georgia Institute of Technology, Atlanta, GA, USA.
authors.forename: John R
authors.initials: JR
authors.lastname: Kirby
mesh_terms: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D016680:Genome, Bacterial; D016254:Mutagenesis, Insertional; D019895:Transposases
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D019895:Transposases