title: Immunomagnetic indirect positive sorting of precartilaginous stem cells from neonatal rat.
abstract: To investigate the technique of sorting high-purity precartilaginous stem cells from rat's perichondrium, neonatal rat's perichondrium cells suspensions were incubated with monoclone antibody of anti-fibroblast growth factor receptor-3 (anti-FGFR-3), and the labeled cells were separated from the suspension in the magnetic field by immuno-beads coated with the second antibody. Purity of the sorted neural stem cells was found to be 93.0%-99.0%, with living cells amounting to 80% -85 %. The magnetic cell sorting system could effectively separate precartilaginous stem cells from perichondrium cell suspensions.
authors.affiliation: Department of Orthopedics, Tongji Hospital, Tongji Medical College of Huangzhong University of Science & Technology.
authors.forename: Hao
authors.initials: H
authors.lastname: Cheng
authors.forename: Anmin
authors.initials: A
authors.lastname: Chen
authors.forename: Hongbo
authors.initials: H
authors.lastname: You
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002356:Cartilage / Q000166:cytology*; D002478:Cells, Cultured; D018189:Immunomagnetic Separation / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology*