title: Generation, annotation, and analysis of ESTs from four different Trichoderma strains grown under conditions related to biocontrol.
abstract: The functional genomics project "TrichoEST" was developed focused on different taxonomic groups of Trichoderma with biocontrol potential. Four cDNA libraries were constructed, using similar growth conditions, from four different Trichoderma strains: Trichoderma longibrachiatum T52, Trichoderma asperellum T53, Trichoderma virens T59, and Trichoderma sp. T78. In this study, we present the analysis of the 8,160 expressed sequence tags (ESTs) generated. Each EST library was independently assembled and 1,000-1,300 unique sequences were identified in each strain. First, we queried our collection of ESTs against the NCBI nonredundant database using the BLASTX algorithm. Moreover, using the Gene Ontology hierarchy, we performed the annotation of 40.9% of the unique sequences. Later, based on the EST abundance, we examined the highly expressed genes in the four strains. A hydrophobin was found as the gene expressed at the highest level in two of the strains, but we also found that other unique sequences similar to the HEX1, QID3, and NMT1 proteins were highly represented in at least two of the Trichoderma strains.
authors.affiliation: Centro de Investigaciones Científicas Isla de la Cartuja, Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC/University of Seville, 41092 Sevilla, Spain. javizca@usal.es
authors.forename: Juan Antonio
authors.initials: JA
authors.lastname: Vizcaíno
authors.forename: José
authors.initials: J
authors.lastname: Redondo
authors.forename: M Belén
authors.initials: MB
authors.lastname: Suárez
authors.forename: Rosa Elena
authors.initials: RE
authors.lastname: Cardoza
authors.forename: Rosa
authors.initials: R
authors.lastname: Hermosa
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: González
authors.forename: Manuel
authors.initials: M
authors.lastname: Rey
authors.forename: Enrique
authors.initials: E
authors.lastname: Monte
mesh_terms: D030541:Databases, Genetic; D020224:Expressed Sequence Tags; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015723:Gene Library; D023281:Genomics; D008969:Molecular Sequence Data; D010572:Pest Control, Biological; D017422:Sequence Analysis, DNA; D014242:Trichoderma / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
chemical_list: D005656:Fungal Proteins