title: Diversity of Ca2+-induced morphology revealed by morphological phenotyping of Ca2+-sensitive mutants of Saccharomyces cerevisiae.
abstract: Yeast cell morphology can be treated as a quantitative trait using the image processing software CalMorph. In the present study, we investigated Ca(2+)-induced morphological changes in Ca(2+)-sensitive (cls) mutants of Saccharomyces cerevisiae, based on the discovery that the characteristic Ca(2+)-induced morphological changes in the Ca(2+)-sensitive mutant zds1 reflect changes in the Ca(2+) signaling-mediated cell cycle control pathway. By applying hierarchical cluster analysis to the quantitative morphological data of 58 cls mutants, 31 of these mutants were classified into seven classes based on morphological similarities. The patterns of morphological change induced by Ca(2+) in one class differed from those of another class. Based on the results obtained using versatile methods for phenotypic analysis, we conclude that a high concentration of Ca(2+) exerts a wide variety of effects on yeast and that there are multiple Ca(2+)-regulatory pathways that are distinct from the Zds1p-related pathway.
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Bldg. FSB-101, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan.
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Ohnuki
authors.forename: Satoru
authors.initials: S
authors.lastname: Nogami
authors.forename: Hanako
authors.initials: H
authors.lastname: Kanai
authors.forename: Dai
authors.initials: D
authors.lastname: Hirata
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Nakatani
authors.forename: Shinichi
authors.initials: S
authors.lastname: Morishita
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Ohya
mesh_terms: D002118:Calcium / Q000494:pharmacology*; D016000:Cluster Analysis; D008954:Models, Biological; D050505:Mutant Proteins / Q000302:isolation & purification; D009154:Mutation / Q000235:genetics*; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000187:drug effects*; D029701:Saccharomyces cerevisiae Proteins / Q000302:isolation & purification
chemical_list: D050505:Mutant Proteins; D029701:Saccharomyces cerevisiae Proteins; D002118:Calcium