Control of frictional coupling of transmembrane cell receptors in model cell membranes with linear polymer spacers.
The planar plasma membrane model with linear polymer spacers with defined lengths enables the control of the frictional coupling between incorporated transmembrane proteins (human platelet integrin) and the solid substrate. This mimics the viscous environment provided by the extracellular matrix of cells. The friction coefficient can be calculated quantitatively from the diffusion coefficient of integrin, measured by fluorescence recovery after photobleaching. The obtained results demonstrate a clear influence of the length and lateral density of polymer chains on the mobility of transmembrane proteins.
Purrucker
Oliver
O
Lehrstuhl für Biophysik E22, Technische Universität München, D85748 Garching, Germany.
Förtig
Anton
A
Jordan
Rainer
R
Sackmann
Erich
E
Tanaka
Motomu
M
D002462:Cell Membrane / Q000737:chemistry*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004058:Diffusion; D005109:Extracellular Matrix / Q000737:chemistry; D006801:Humans; D066298:In Vitro Techniques; D016023:Integrins / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry; D008956:Models, Chemical; D038761:Photobleaching; D011956:Receptors, Cell Surface; D013050:Spectrometry, Fluorescence; D014783:Viscosity
D016023:Integrins; D008565:Membrane Proteins; D011956:Receptors, Cell Surface