title: Evolutionary divergence of the pre-promotor region of ribosomal DNA in the great apes.
abstract: The human ribosomal intergenic spacer (rIGS) differs considerably on nucleotide sequence and regulatory elements positioning from their counterparts in the mouse, rat and Xenopus laevis. In the present study, we have PCR amplified, cloned and sequenced the rIGS fragments of about 4.5 kb length, located approximately 2 kb upstream of the rRNA transcription start point for the great apes, Pan paniscus, Pan troglodytes, Gorilla gorilla and Pongo pygmaeus. Alignment of the primates' orthologic nucleotide sequences reveals high extent of similarity, with the exception of highly repetitious region between the two Alu repeats, nearest to the onset of transcription. Data obtained have been analyzed for further understanding of the evolution of repetitive sequences. We have also shown, that MARs/SARs distribution patterns in the pre-promoter rIGSs of the great apes and the mouse are surprisingly similar in spite of an absence of similarity in the primary structure and regulatory elements organization in the region under study.
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Kirill K
authors.initials: KK
authors.lastname: Netchvolodov
authors.forename: Anastasia V
authors.initials: AV
authors.lastname: Boiko
authors.forename: Alexei P
authors.initials: AP
authors.lastname: Ryskov
authors.forename: Natalia S
authors.initials: NS
authors.lastname: Kupriyanova
mesh_terms: D000818:Animals; D001483:Base Sequence; D004275:DNA, Ribosomal / Q000235:genetics*; D019143:Evolution, Molecular; D006071:Gorilla gorilla; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D019974:Pan paniscus; D002679:Pan troglodytes; D011148:Pongo pygmaeus; D011401:Promoter Regions, Genetic
chemical_list: D004275:DNA, Ribosomal