title: Conducting polymers in modelling transient potential of biological membranes.
abstract: The possibility of using conducting polymer (CP) films doped with biological ligands as artificial biological membranes to study potential formation mechanisms is presented. Calcium and magnesium ion-binding anionic sites--asparagine, glutamine, adenosinotriphosphate and heparin are incorporated into the poly(pyrrole) film during electrochemical polymerization. This approach allows the competitive calcium-magnesium ion-exchange to be inspected by open circuit measurements. After a close-to-Nernstian sensitivity of the CP membranes was induced by soaking in alkaline solutions of calcium or magnesium, dynamic experiments were performed by a change in the bulk concentration of magnesium or calcium ions. A characteristic transitory potential response, though distinctively different for the calcium and magnesium ions, was observed and explained using the diffusion layer model (DML).
authors.affiliation: Faculty of Material Science and Ceramics, AGH-University of Science and Technology, Al. Mickiewicza 30, PL-30059 Cracow, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Paczosa-Bator
authors.forename: Teresa
authors.initials: T
authors.lastname: Blaz
authors.forename: Jan
authors.initials: J
authors.lastname: Migdalski
authors.forename: Andrzej
authors.initials: A
authors.lastname: Lewenstam
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry; D001216:Asparagine / Q000737:chemistry; D002118:Calcium / Q000737:chemistry*; D002413:Cations, Divalent / Q000737:chemistry; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D004058:Diffusion; D004563:Electrochemistry; D005973:Glutamine / Q000737:chemistry; D006493:Heparin / Q000737:chemistry; D007474:Ion Exchange; D008024:Ligands; D008274:Magnesium / Q000737:chemistry*; D008954:Models, Biological; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry; D012680:Sensitivity and Specificity; D012996:Solutions / Q000737:chemistry; D013057:Spectrum Analysis; D013499:Surface Properties; D013997:Time Factors
chemical_list: D002413:Cations, Divalent; D008024:Ligands; D011108:Polymers; D011758:Pyrroles; D012996:Solutions; D005973:Glutamine; D001216:Asparagine; D000255:Adenosine Triphosphate; D006493:Heparin; D008274:Magnesium; D002118:Calcium