Role of cumulus cells on in vitro maturation of canine oocytes.
The objectives of the present study were to investigate the relationship between the morphological status of cumulus cells surrounding canine oocytes after maturation culture and the meiotic stage of the oocytes. In addition, the effect of the removal of cumulus cells from canine cumulus-oocyte complexes (COCs) during maturation culture on their meiotic competence was examined. Canine COCs were collected from bitches at the anoestrous and dioestrous stages and only COCs with >110 microm in vitelline diameter were cultured in medium 199 with 10% canine serum for 72 h. In the first experiment, the relation between the morphological status of cumulus cells surrounding oocytes cultured for 72 h and their meiotic stages was examined. At the end of maturation culture, the proportions of intact, partially nude and completely nude oocytes were 65.2%, 22.9% and 11.9%, respectively. The proportion of maturation to metaphase II of completely nude oocytes was highest among the oocytes with different morphological status of cumulus cells. In the second experiment, the cumulus cells were partially or completely removed from COCs at 48 h after the start of maturation culture and the oocytes were cultured for a further 24 h. The proportion of oocytes reaching metaphase II in the completely denuded oocytes was significantly higher than that in the control oocytes without the removal treatment of cumulus cells. The results indicate that morphological status of cumulus cells surrounding oocytes may be related to the nuclear maturation of canine oocytes, and the removal of cumulus cells from COCs during maturation culture can promote the completion of oocyte meiotic maturation.
Otoi
T
T
Department of Physiology and Pharmacology, College of Veterinary Medicine, Texas A&M University, College Station, TX , USA. otoi@yamaguchi-u.ac.jp
Shin
T
T
Kraemer
D C
DC
Westhusin
M E
ME
D000780:Anestrus; D000818:Animals; D002478:Cells, Cultured; D004285:Dogs / Q000502:physiology*; D030762:Estrous Cycle / Q000502:physiology*; D005260:Female; D008540:Meiosis / Q000502:physiology*; D009865:Oocytes / Q000502:physiology*; D009866:Oogenesis; D013997:Time Factors