Proteome changes in leaves from grapevine (Vitis vinifera L.) transformed for alcohol dehydrogenase activity.
A proteomic approach has been used to study changes in leaf protein content from plants transformed for alcohol dehydrogenase (ADH) activity. Individual quantitative analysis of 190-436 spots separated by two-dimensional electrophoresis was performed, and spots displaying significant quantitative changes between control (C), sense (S), and antisense (R) transformants were selected using Student's t test. Of the 14 spots selected and further analyzed after trypsic digestion, 9 could be identified by MS analysis and 5 by LC-MS/MS. Identified proteins had mainly a chloroplastic origin: four rubisco large subunits, one rubisco binding protein, two glutamine synthetases, one elongation factor Tu, one ATP synthase beta subunit, and one plastidic aldolase. Proteins with other localization were also identified, such as a UDP-glucose pyrophosphorylase, a mitochondrial aminomethyltransferase, a linalool synthase, which comigrated with the protein identified as elongation factor Tu, an enolase comigrating with a glyceraldehyde 3-phosphate dehydrogenase, and a mixture of eight proteins among which were a dehydroascorbate reductase, a chalcone isomerase, and a rubisco activase. The results emphasize the changes in carbon metabolism-associated proteins linked to the alteration in ADH activity of grapevine transformant leaves.
Sauvage
François-Xavier
FX
UMR 1083, Sciences Pour l'Oenologie, Institut National de la Recherche Agronomique, 2 Place Viala, F-34060 Montpellier cedex 01, France.
Pradal
Martine
M
Chatelet
Philippe
P
Tesniere
Catherine
C
D000426:Alcohol Dehydrogenase / Q000235:genetics* / Q000378:metabolism*; D013058:Mass Spectrometry; D018515:Plant Leaves / Q000737:chemistry*; D010940:Plant Proteins / Q000032:analysis*; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000201:enzymology*; D027843:Vitis / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics
D010940:Plant Proteins; D000426:Alcohol Dehydrogenase