title: Maillard reaction products enriched food extract reduce the expression of myofibroblast phenotype markers.
abstract: Advanced glycation end products (AGE) are associated with a wide range of degenerative diseases. The present investigation aimed at analysing the influence of AGE containing nutritional extracts on cardiac fibroblasts (CFs) as the major cell type responsible for cardiac fibrosis. Mice CFs were treated with bread crust extract (BCE) which contained significant amounts of a variety of AGE modifications. BCE treatment with up to 30 mg/mL did not impair cell viability. Furthermore, BCE induced a moderate elevation of reactive oxygen species (ROS) production and activation of redox sensitive pathways like the p42/44(MAPK), p38(MAPK) and NF-kappaB but did not alter Akt kinase phosphorylation. Expression of smooth muscle alpha-actin and tropomyosin-1, which represent markers for myofibroblast differentiation, was reduced after bread crust treatment. These data suggest a putative antifibrotic effect of melanoidin-rich food.
authors.affiliation: Department of Cardiothoracic Surgery, University of Halle-Wittenberg, Halle, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Ruhs
authors.forename: Norbert
authors.initials: N
authors.lastname: Nass
authors.forename: Veronika
authors.initials: V
authors.lastname: Somoza
authors.forename: Ulrich
authors.initials: U
authors.lastname: Friess
authors.forename: Reinhard
authors.initials: R
authors.lastname: Schinzel
authors.forename: Rolf-Edgar
authors.initials: RE
authors.lastname: Silber
authors.forename: Andreas
authors.initials: A
authors.lastname: Simm
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001939:Bread; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D016326:Extracellular Matrix Proteins / Q000235:genetics; D005347:Fibroblasts / Q000737:chemistry* / Q000187:drug effects*; D005502:Food; D015870:Gene Expression / Q000187:drug effects; D017127:Glycation End Products, Advanced / Q000494:pharmacology*; D015416:Maillard Reaction; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D009206:Myocardium / Q000166:cytology*; D016328:NF-kappa B / Q000378:metabolism; D010641:Phenotype; D011108:Polymers / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000032:analysis; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D015415:Biomarkers; D016326:Extracellular Matrix Proteins; D017127:Glycation End Products, Advanced; D016328:NF-kappa B; D011108:Polymers; D017382:Reactive Oxygen Species; C011908:melanoidin polymers; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D048051:p38 Mitogen-Activated Protein Kinases