title: Fatty acid efficiency profile in uncoupling of Acanthamoeba castellanii mitochondria.
abstract: A profile of free fatty acid (FFA) specificity in Acanthamoeba castellanii mitochondrial uncoupling is described. The FFA uncoupling specificity was observed as different abilities to stimulate resting respiration, to decrease resting membrane potential, and to decrease oxidative phosphorylation efficiency. Tested unsaturated FFA (C18-20) were more effective as uncouplers and protonophores when compared to tested saturated FFA (C8-18), with palmitic acid (C16:0) as the most active. As FFA efficiency in mitochondrial uncoupling is related to physiological changes of fatty acid composition (and thereby FFA availability) during growth of amoeba cells, it could be a way to regulate the activity of an uncoupling protein and thereby the efficiency of oxidative phosphorylation during a cell life of this unicellular organism.
authors.affiliation: Laboratory of Bioenergetics, Adam Mickiewicz University, Fredry 10, 61-701 Poznan, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Swida
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Czarna
authors.forename: Andrzej
authors.initials: A
authors.lastname: Woyda-Płoszczyca
authors.forename: Anna
authors.initials: A
authors.lastname: Kicinska
authors.forename: Francis E
authors.initials: FE
authors.lastname: Sluse
authors.forename: Wieslawa
authors.initials: W
authors.lastname: Jarmuszkiewicz
mesh_terms: D048248:Acanthamoeba castellanii / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D019069:Cell Respiration / Q000187:drug effects; D005227:Fatty Acids / Q000378:metabolism / Q000494:pharmacology*; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology*; D010085:Oxidative Phosphorylation / Q000187:drug effects; D019308:Palmitic Acid / Q000378:metabolism / Q000494:pharmacology*; D014475:Uncoupling Agents / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D005227:Fatty Acids; D014475:Uncoupling Agents; D019308:Palmitic Acid