title: Chromosomal 20q gain in the DNA diploid component of aneuploid colorectal carcinomas.
abstract: The order of appearance of different genetic aberrations during the shift from diploidy/near-diploidy to aneuploidy in colorectal cancers is not yet clear. We studied genetic alterations in flow cytometrically-sorted DNA diploid and corresponding aneuploid epithelial cell populations from each of 20 colorectal tumors using comparative genomic hybridization, FISH, and PCR. Analysis of the 19 cases in which aberrations were found in the flow-sorted diploid population indicated that large-scale aneuploidization in colorectal cancer was preceded by amplification of oncogene(s) localized to chromosome 20q13.2 and by KRAS mutations, but not by TP53 deletions or losses of large chromosomal regions such as 4q, 8p and 18q.
authors.affiliation: The Pathology Clinic, University of Oslo, Rikshospitalet-Radiumhospitalet Medical Center, 0027 Oslo, Norway. p.d.angelis@medisin.uio.no
authors.forename: Paula M
authors.initials: PM
authors.lastname: De Angelis
authors.forename: Trond
authors.initials: T
authors.lastname: Stokke
authors.forename: Marzieh
authors.initials: M
authors.lastname: Beigi
authors.forename: Gøril
authors.initials: G
authors.lastname: Flatberg
authors.forename: Marianne
authors.initials: M
authors.lastname: Enger
authors.forename: Kristiane
authors.initials: K
authors.lastname: Haug
authors.forename: Hans Christian D
authors.initials: HC
authors.lastname: Aass
authors.forename: Aasa
authors.initials: A
authors.lastname: Schjølberg
authors.forename: Per Arne
authors.initials: PA
authors.lastname: Andresen
authors.forename: Sarah
authors.initials: S
authors.lastname: Ariansen
authors.forename: Anne Signe
authors.initials: AS
authors.lastname: Bø
authors.forename: Odd
authors.initials: O
authors.lastname: Mjåland
authors.forename: Ole Petter
authors.initials: OP
authors.lastname: Clausen
mesh_terms: D000782:Aneuploidy; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D002869:Chromosome Aberrations; D002890:Chromosomes, Human, Pair 20 / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics / Q000473:pathology*; D004252:DNA Mutational Analysis; D004171:Diploidy; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D005434:Flow Cytometry; D011905:Genes, ras / Q000235:genetics; D015894:Genome, Human; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D019656:Loss of Heterozygosity; D009154:Mutation; D009693:Nucleic Acid Hybridization / Q000379:methods; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
chemical_list: D016159:Tumor Suppressor Protein p53