8-Isoprostane in nasally exhaled breath condensate in different pediatric lung diseases.
OBJECTIVE
Increased levels of 8-isoprostane were found in various human lung diseases suggesting 8-isoprostane as a marker of pulmonary oxidative stress in vivo. The exact role in pediatric lung diseases has not been defined yet. The goal of this study was to clarify the role of 8-isoprostane in nasally exhaled breath condensate as possible marker of oxidative stress in children with different lung diseases.


METHODS
Levels of 8-isoprostane were measured in nasally exhaled breath condensate of 29 cystic fibrosis patients, 19 children with a history of wheezing episodes, 8 infants with acute respiratory tract infection and 53 healthy subjects using a specific enzyme immunoassay.


RESULTS
Levels of 8-isoprostane did neither discriminate between different disease groups nor correlate with lung function in cystic fibrosis patients.


CONCLUSIONS
Levels of 8-isoprostane in nasally exhaled breath condensate do not reflect oxidative stress in children with different lung diseases.
Latzin
P
P
Dr. von Haunersches Kinderspital, Ludwig-Maximilians-University, Lindwurmstr. 4, 80337 Munich, Germany.
Beck-Ripp
J
J
Hartl
D
D
Bartenstein
A
A
Noss
J
J
Griese
Matthias
M
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D015415:Biomarkers / Q000032:analysis; D001944:Breath Tests; D002648:Child; D002675:Child, Preschool; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000032:analysis; D006801:Humans; D007118:Immunoassay; D007223:Infant; D008171:Lung Diseases / Q000378:metabolism*; D018384:Oxidative Stress; D012129:Respiratory Function Tests; D018709:Statistics, Nonparametric
D015415:Biomarkers; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost