title: [The mechanism of interaction between Yersinia pestis and erythrocytes, and its importance for the pathogenesis of plague].
abstract: The ability of Yersinia pestis to get inside human and murine red blood cells (RBC) was found both in vivo and in vitro experiments. Due to oxidase and catalase activities, the microorganisms induced the denaturation of hemoglobin (Hb) through RBC oxidation to H2O2 in high concentration providing the formation of haemin and transformation of haem Fe2+ into the utilizable form, Fe3+. This phenomenon was found to be common in vitro for all Y. pestis strains used in the study independently of Pgm phenotype and plasmid content, including vaccine Pgm(-) Y. pestis EV NIIEG and plasmidless Pgm(+) Y. pestis PKR-133 stains. This, probably, allows the bacteria to use Hb as an essential source of iron and porphyrins for de novo synthesis of DNA followed by effective multiplication in the mammalian organism. A correlation between the loss of the ability of RBC to transport O2 to organs and tissues and the development of progressive tissue hypoxia with specific clinical features of metHb accumulation and haemorrhagic syndrome was shown. The participation of Y. pestis phospholipases (A and D) in the destruction of RBC membranes and translocation of plague bacilli into RBC, as well as the phenomenon of polysaccharide chain lengthening depending on cultivation conditions of Y. pestis bacteria, are discussed.
authors.forename: V A
authors.initials: VA
authors.lastname: Fedorova
authors.forename: Z L
authors.initials: ZL
authors.lastname: Devdariani
mesh_terms: D000818:Animals; D001692:Biological Transport; D002374:Catalase / Q000378:metabolism; D004269:DNA, Bacterial / Q000096:biosynthesis; D018450:Disease Progression; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism / Q000382:microbiology*; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010930:Plague / Q000097:blood / Q000382:microbiology*; D015010:Yersinia pestis / Q000235:genetics / Q000472:pathogenicity*
chemical_list: D004269:DNA, Bacterial; D010088:Oxidoreductases; D002374:Catalase; D010100:Oxygen