Kinetics of the plastoquinone pool oxidation following illumination Oxygen incorporation into photosynthetic electron transport chain.
The oxidation of the PQ-pool after illumination with 50 or 500 micromol quantam(-2)s(-1) was measured in isolated thylakoids as the increase in DeltaA(263), i.e., as the appearance of PQ. While it was not observed under anaerobic conditions, under aerobic conditions it was biphasic. The first faster phase constituted 26% or 44% of total reappearance of PQ, after weak or strong light respectively. The dependence on oxygen presence as well as the correlation with the rate of oxygen consumption led to conclusion that this phase represents the appearance of PQ from PQ(*-) produced in the course of PQH(2) oxidation by superoxide accumulated in the light within the membrane.
Ivanov
Boris
B
Institute of Basic Biological Problems, Russian Academy of Sciences, Photosynthetic Electron Transport Lab, Pushchino 142290, Moscow Region, Russian Federation.
Mubarakshina
Maria
M
Khorobrykh
Sergey
S
D004579:Electron Transport; D007700:Kinetics; D008027:Light; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D018532:Pisum sativum / Q000378:metabolism / Q000528:radiation effects; D010775:Photic Stimulation; D010788:Photosynthesis; D010971:Plastoquinone / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism; D020524:Thylakoids / Q000378:metabolism* / Q000528:radiation effects
C100460:plastosemiquinone; D013481:Superoxides; C003165:plastoquinol; D010971:Plastoquinone; D010100:Oxygen