[Matrine-induced gamma-globin synthesis in K562 cells].
OBJECTIVE
To study the effects of matrine on gamma-globin synthesis in K562 cells in vitro.


METHODS
Benzidine staining was used to study the dose- and time-dependent effects of matrine on hemoglobin synthesis in K562 cells, and Western blotting was performed to determine the level of hemoglobin F(alpha(2)gamma(2)).


RESULTS
Benzidine staining showed that K562 cells treated with matrine of 0.1 mg/ml had a positivity rate for benzidine (BZ%) of 15.67% at 96 h after the treatment, and Western blotting indicated increased synthesis of hemoglobin F.


CONCLUSIONS
Matrine can induce gamma-globin synthesis and increase hemoglobin F level in K562 cells, the effect of which resembles that of sodium butyrate.
Zhang
Cui-Mei
CM
Department of Pediatrics, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. zhangcuimei0527@163.com
Feng
Zhi-Chun
ZC
D000470:Alkaloids / Q000494:pharmacology*; D015153:Blotting, Western; D005319:Fetal Hemoglobin / Q000096:biosynthesis; D005914:Globins / Q000096:biosynthesis*; D006801:Humans; D020014:K562 Cells; D011807:Quinolizines / Q000494:pharmacology*; D000093842:Matrines
D000470:Alkaloids; D011807:Quinolizines; D005914:Globins; D005319:Fetal Hemoglobin; D000093842:Matrines