title: [P-JAK2 and P-STAT3 protein expression and cell apoptosis following focal cerebral ischemia-reperfusion injury in rats].
abstract: OBJECTIVE
  To investigate the changes in phosphorylated JAK2 and STAT3 protein expression of and cell apoptosis following focal cerebral ischemia-reperfusion injury in rats.
  
  
  METHODS
  A rat models of focal cerebral ischemia-reperfusion injury was established by middle cerebral artery occlusion using modified filament method. Immunohistochemistry and Western blot analysis were used to detect the expression of P-JAK2 and P-STAT3 proteins, and TUNEL assay was employed to examine the cell apoptosis.
  
  
  RESULTS
  P-JAK2 and P-STAT3 protein expression increased significantly after cerebral ischemia-reperfusion injury in rats. The immunoreactivity was prominent in the peripheral of the ischemic region and reached the peak level at 24 h of reperfusion, followed by slight decrement. The apoptotic cells increased obviously after cerebral ischemia-reperfusion injury, also reaching the peak level at 24 h of reperfusion.
  
  
  CONCLUSIONS
  The expression of phosphorylated JAK2 and STAT3 may be involved in the ischemic cellular events including apoptosis. JAK2/STAT3 signaling pathway plays a role in the pathophysiological process of cerebral ischemia/reperfusion cell injury and repair.
authors.affiliation: Department of Neurology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. xhffhx@126.com
authors.forename: Hui-Fang
authors.initials: HF
authors.lastname: Xie
authors.forename: Ru-Xiang
authors.initials: RX
authors.lastname: Xu
authors.forename: Ji-Peng
authors.initials: JP
authors.lastname: Wei
authors.forename: Xiao-Dan
authors.initials: XD
authors.lastname: Jiang
authors.forename: Zhen-Hua
authors.initials: ZH
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D020244:Infarction, Middle Cerebral Artery / Q000503:physiopathology*; D053614:Janus Kinase 2 / Q000378:metabolism*; D008297:Male; D010766:Phosphorylation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000235:genetics / Q000503:physiopathology*; D050796:STAT3 Transcription Factor / Q000378:metabolism*
chemical_list: D050796:STAT3 Transcription Factor; D053614:Janus Kinase 2