title: [Potassium channel blocker regulates the proliferation of prostatic epithelial cells in SD rats].
abstract: OBJECTIVE
  To investigate the regulatory effect of potassium channel blocker (tetraethylammonium [TEA], aminopyridine [4-AP], glibenclamide [Glib]) on the proliferation of SD rat prostatic epithelial cells in vitro.
  
  
  METHODS
  The primary culture was prepared by collagenase dissociation of minced prostatic tissues. Cells were cultured in serum-free prostate epithelial cell growth media and identified by immunocytochemical studies. TEA and 4-AP at the concentration of 1, 5 and 10 mmol/L and Glib at the concentration of 10, 50 and 100 mol/L were added, and after 24, 48 and 72 hours of culturing, a cell column diagram was drawn and the cell number counted. The post-passage cell growth was observed by MTT assay and Hoechst33258 nucleus staining.
  
  
  RESULTS
  The cultured cells showed the typical morphological features of epithelia, with positive stain. MTT assay and Hoechst33258 staining showed that TEA, 4-AP and Glib at the increasing concentration effected different degrees of proliferation of prostatic epithelial cells after 24, 48 and 72 h (P < 0.01).
  
  
  CONCLUSIONS
  The potassium channel blocker is a direct physiological regulator of the proliferation of SD rat prostatic epithelial cells.
authors.affiliation: Department of Urology, First Affiliated Hospital of Anhui Medical University, China.
authors.forename: Zheng-xing
authors.initials: ZX
authors.lastname: Zhou
authors.forename: Chao-zhao
authors.initials: CZ
authors.lastname: Liang
authors.forename: Zhi-guo
authors.initials: ZG
authors.lastname: Tang
authors.forename: Zong-yao
authors.initials: ZY
authors.lastname: Hao
authors.forename: Qing-kui
authors.initials: QK
authors.lastname: Guo
authors.forename: Yong
authors.initials: Y
authors.lastname: Hu
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects*; D008297:Male; D026902:Potassium Channel Blockers / Q000494:pharmacology*; D011467:Prostate / Q000166:cytology* / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D026902:Potassium Channel Blockers