Fully packed capillary electrochromatographic microchip with self-assembly colloidal silica beads.
A fully packed capillary electrochromatographic (CEC) microchip showing improved solution and chip handling was developed. Microchannels for the CEC microchip were patterned on a cyclic olefin copolymer substrate by injection molding and packed fully with 0.8-microm monodisperse colloidal silica beads utilizing a self-assembly packing technique. The silica packed chip substrate was covered and thermally press-bonded. After fabrication, the chip was filled with buffer solution by self-priming capillary action. The self-assembly packing at each channel served as a built-in nanofilter allowing quick loading of samples and running buffer solution without filtration. Because of a large surface area-to-volume ratio of the silica packing, reproducible control of electroosmotic flow was possible without leveling of the solutions in the reservoirs resulting 1.3% rsd in migration rate. The capillary electrophoretic separation characteristics of the chip were studied using fluorescein isothiocyanate (FITC)-derivatized amino acids as probe molecules. A mixture of FITC and four FITC-derivatized amino acids was successfully separated with 2-mm separation channel length.
Park
Jongman
J
Analytical Laboratory, Department of Chemistry, Konkuk University, 1 Hwayangdong, Gwangjingu, Seoul 143-701, Korea. chong.ahn@uc.edu
Lee
Dami
D
Kim
Won
W
Horiike
Shigeyoshi
S
Nishimoto
Takahiro
T
Lee
Se Hwan
SH
Ahn
Chong H
CH
D000596:Amino Acids / Q000737:chemistry; D053801:Capillary Electrochromatography / Q000295:instrumentation / Q000379:methods*; D003102:Colloids; D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives; D012822:Silicon Dioxide / Q000737:chemistry*
D000596:Amino Acids; D003102:Colloids; D012822:Silicon Dioxide; D016650:Fluorescein-5-isothiocyanate