title: [Analysis of gene expression of fatty acid synthase in squamous cell carcinoma of the oral cavity by the method of real-time quantitative PCR].
abstract: OBJECTIVE
  To relatively quantify the gene expression of fatty acid synthase in squamous cell carcinoma, adjacent tissue, and some normal oral tissues by real-time quantitative PCR.
  
  
  METHODS
  The tissues were collected fresh from surgical specimens. The collected tissues were minced. Then the total RNA was extracted. The RNA was reversely transcripted into cDNA with random prime. And then the cDNA was amplified by real-time quantitative PCR to quantify the gene expression of FAS according to an internal control GAPDH. The difference of FAS gene expression was compared between squamous cell carcinoma, adjacent tissue, and some normal oral tissues.
  
  
  RESULTS
  The expression of FAS of squamous cell carcinoma was notably higher than the other two (P < 0.001).
  
  
  CONCLUSIONS
  Real-time quantitative PCR provides a method for monitoring the expression of fatty acid synthetic activity in squamous cell carcinoma, adjacent and normal tissues.
authors.affiliation: Key Laboratory of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China.
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Wang
authors.forename: Xiao-Yi
authors.initials: XY
authors.lastname: Wang
authors.forename: Qing-Hong
authors.initials: QH
authors.lastname: Gao
authors.forename: Xiao-Yong
authors.initials: XY
authors.lastname: Yang
authors.forename: Hong-Ping
authors.initials: HP
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Zeng
mesh_terms: D002294:Carcinoma, Squamous Cell; D064429:Fatty Acid Synthases; D006801:Humans; D009062:Mouth Neoplasms; D016133:Polymerase Chain Reaction
chemical_list: D064429:Fatty Acid Synthases