title: Studies on genome relationship and species-specific PCR marker for Dasypyrum breviaristatum in Triticeae.
abstract: Dasypyrum breviaristatum and nine related species in Triticeae were analyzed using the random amplified polymorphic DNA (RAPD) technique, in order to understand the genetic relationship and to develop species specific markers. The genome relationship dendrogram shows that D. breviaristatum and D. villosum could not be grouped together, indicating that D. breviaristatum was unlikely to be directly derived from D. villosum, while D. breviaristatum was closest to Thinopyrum intermedium, which implied that they might have similar breeding behaviors when introducing their chromatins into wheat. A D. breviaristatum genome specific RAPD product of 1182bp, was cloned and designated as pDb12H. Sequence analysis revealed that pDb12H was strongly homologuos to a long terminal repeat (LTR) Sabrina retrotransposon newly reported in Hordeum. The pDb12H was converted into a PCR based marker, which allows effectively monitoring the D. breviaristatum chromatin introgression into wheat. Fluorescence in situ hybridization (FISH) suggested that pDb12H was specifically hybridized throughout all D. breviaristatum chromosomes arms except for the terminal and centromeric regions, which can be used to characterize wheat -D. breviaristatum chromosome translocation. The genomes repetitive element will also be useful to study gene interactions between the wheat and alien genomes after the polyploidization.
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, Sichuan, PR China. yangzujun@uestc.edu.cn
authors.forename: Zu-Jun
authors.initials: ZJ
authors.lastname: Yang
authors.forename: Cheng
authors.initials: C
authors.lastname: Liu
authors.forename: Juan
authors.initials: J
authors.lastname: Feng
authors.forename: Guang-Rong
authors.initials: GR
authors.lastname: Li
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Zhou
authors.forename: Ke-Jun
authors.initials: KJ
authors.lastname: Deng
authors.forename: Zheng-Long
authors.initials: ZL
authors.lastname: Ren
mesh_terms: D018744:DNA, Plant / Q000378:metabolism; D005819:Genetic Markers; D018745:Genome, Plant; D017404:In Situ Hybridization, Fluorescence; D006109:Poaceae / Q000145:classification* / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014908:Triticum / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers