title: Prevalence of Toxocara-induced liver granulomas, detected by immunohistochemistry, in a series of autopsies at a Children's Reference Hospital in Vitoria, ES, Brazil.
abstract: The aim of this investigation was to study the frequency of visceral larva migrans (VLM) granulomas in autopsies at a Children's Reference Hospital in Vitoria, ES Brazil, where anti-Toxocara antibodies are frequently detected in the serum of children admitted at the hospital. Two liver fragments from 310 autopsies of children aged between 1 and 15 years were paraffin embedded, and sections were stained with hematoxylin and eosin and submitted to detection of Toxocara antigens using a rabbit anti-Toxocara serum. Among the 24 cases with granulomatous lesions, ten had eosinophil-rich granulomas positively stained with the anti-Toxocara serum. Some were typical epithelioid granulomas, with a positive reaction in multinucleated giant cells, epithelioid cells, or necrotic debris. The results showed that VLM granulomas are the most frequent granulomatous hepatitis in children in our county. This agrees with the high prevalence of anti-Toxocara antibodies in the serum of children admitted to the Children's Reference Hospital. The 3.2% frequency of liver VLM granulomas in autopsies is less than the 30-39% frequency of positive serology in these children, probably reflecting the low larval burden in infected children.
authors.affiliation: Núcleo de Doenças Infecciosas, Centro Biomédico, UFES, Av Marechal Campos 1468, 29040-091, Vitória, ES, Brazil. carmusso@veloxmail.com.br
authors.forename: Carlos
authors.initials: C
authors.lastname: Musso
authors.forename: Jane S
authors.initials: JS
authors.lastname: Castelo
authors.forename: Ana M C
authors.initials: AM
authors.lastname: Tsanaclis
authors.forename: Fausto E L
authors.initials: FE
authors.lastname: Pereira
mesh_terms: D000293:Adolescent; D000818:Animals; D001344:Autopsy; D001938:Brazil / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D005260:Female; D006099:Granuloma / Q000453:epidemiology* / Q000276:immunology / Q000469:parasitology; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007150:Immunohistochemistry / Q000379:methods*; D007223:Infant; D007816:Larva Migrans, Visceral / Q000453:epidemiology* / Q000276:immunology / Q000473:pathology; D008107:Liver Diseases / Q000453:epidemiology* / Q000276:immunology / Q000469:parasitology; D008297:Male; D015995:Prevalence; D011817:Rabbits; D014119:Toxocara / Q000276:immunology*; D017166:Toxocara canis / Q000276:immunology
chemical_list: D007106:Immune Sera