Anomalous electric birefringence behavior of sonicated DNA fragments as observed in reversing-pulse transients and steady-state sign reversal: a multicomponent approach.
Anomalous electric birefringence signals of a sonicated and column-fractionated medium-size calf thymus DNA sample (bp=570) in Na(+) solutions were measured at 7 degrees C. The reversing-pulse electric birefringence (RPEB) signal pattern was theoretically calculated in the low electric field region for two axially symmetric models coexisting in equilibrium in solution. The RPEB theory is based on the electric dipole moment due to ion-fluctuation along the longitudinal direction and the electric polarizability anisotropy (Deltaalpha'), together with various electric and optical parameters assigned to the models. An analytical method was developed for the steady-state birefringence of the two-component system in a wide range of electric fields. The NaDNA samples exhibit complex RPEB patterns mixed with negative- and positive-going profiles. An experimental RPEB signal of NaDNA at an absorbance (A(260)) of 8 was fitted to theoretical curve at weak electric fields. The anomalous RPEB signal was attributed to the component 2, which shows a dip in the buildup and another in the reverse processes with a positive sign and a larger relaxation time. For the component 1, a normal DNA profile with negative sign is associated with a narrow dip in the reverse and a faster relaxation time in the decay signal. The field-strength dependence of observed steady-state birefringence delta(infinity) could be fitted for NaDNA at A(260)=8 by the SUSID orientation function with saturated ionic and electronic moments. An apparent positive maximum and the sign reversal in delta(infinity) at weak electric fields is an interplay between the positive component 2 with positive optical factor Deltag and negative Deltaalpha' and the negative component 1 with negative Deltag and positive Deltaalpha'. Possible conformation of two DNA components involved in solution was estimated.
Yamaoka
Kiwamu
K
yamaoka@hiroshima-u.ac.jp
D000818:Animals; D016880:Anisotropy; D001718:Birefringence; D002417:Cattle; D002850:Chromatography, Gel; D004247:DNA / Q000737:chemistry*; D003956:Dialysis; D004560:Electricity; D004563:Electrochemistry / Q000379:methods*; D007700:Kinetics; D008027:Light; D008962:Models, Theoretical; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D009994:Osmolar Concentration; D012542:Scattering, Radiation; D012965:Sodium Chloride / Q000737:chemistry; D012996:Solutions; D013010:Sonication; D013056:Spectrophotometry, Ultraviolet; D013950:Thymus Gland / Q000737:chemistry
D012996:Solutions; D012965:Sodium Chloride; D004247:DNA