[Studies on chemical constituents from leaves of Isatis indigotica].
OBJECTIVE
To study the chemical constituents of the leaves of Isatis indigotica.


METHODS
The leaves of I. indigotica were extracted with 80% ethanol. The EtOH extract was dispersed in H20 and extracted with petroleum, EtOAc and BuOH successively. The EtOAc fraction was isolated and purified by column chromatography on silica gel, Sephadex LH -20 and Rp-8, Rp-18. All the compounds were identified on the basis of spectral analyses (including MS, 1H-NMR, 13 C-NMR) , RESULT: Eleven compounds were isolated from the leaves of I. indigotica, and structures were characterized as 10H-indolo [3, 2-b] quinoline (1), indirubin (2), 4 (3H)-quinazo-linone (3), (E)-3-(3', 5'-dimethoxy-4'-hydroxybenzylidene) -2-indolinone (4), 2, 3-dihydropyrrolo [2, 1-b] quinazolin-9(1H) -one (5) , benzoic acid (6) , o-droxy-benzoic acid (7), ( - ) -lariciresinol (8) , ( + ) -isolariciresinol (9), isovitexin (10), 6-f-D-glucopyranosyldiosmetin (11).


CONCLUSIONS
1, 4, 5, 8, 9, 11 were obtained from the leaves of I. indigotica for the first time.
Liu
Ji-feng
JF
State Key Labortatory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, the Chinese Academy of Sciences, Kunming 650204, China.
Zhang
Xue-mei
XM
Xue
Duo-qing
DQ
Jiang
Zhi-yong
ZY
Gu
Qiong
Q
Chen
Ji-jun
JJ
D005663:Furans / Q000737:chemistry / Q000302:isolation & purification; D026121:Indole Alkaloids / Q000737:chemistry / Q000302:isolation & purification; D031219:Isatis / Q000737:chemistry*; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification; D008031:Lignin / Q000737:chemistry / Q000302:isolation & purification; D009284:Naphthols / Q000737:chemistry / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D011804:Quinolines / Q000737:chemistry / Q000302:isolation & purification
D005663:Furans; D026121:Indole Alkaloids; D017705:Lignans; D009284:Naphthols; D011804:Quinolines; C024015:cryptolepine; C060284:isolariciresinol; C060282:lariciresinol; D008031:Lignin