Expression of various CDC25B isoforms in human spermatozoa.
OBJECTIVE
To explore the role of CDC25B protein in postmeiotic germ cells.


METHODS
In vitro experiment.


METHODS
University-based reproductive genetics laboratory.


METHODS
Fertile and infertile volunteers.


METHODS
Reverse transcription-polymerase chain reaction (RT-PCR), real-time RT-PCR, and immunostaining for CDC25B.


METHODS
Expression profiling of CDC25B in human spermatozoa.


RESULTS
Four splicing variants (CDC25B1, B2, B3, and B4) are expressed in human spermatozoa. Immunofluorescence staining showed strong homogeneous staining in the midpiece of spermatozoa, and weak staining in the principal piece and cytosol of the head. The messenger RNA (mRNA) transcript level of CDC25B was increased in sperm samples of men with asthenospermia.


CONCLUSIONS
The expression of CDC25B in different cellular compartments of human spermatozoa suggests that there are diverse noncell-cycle-related functions of CDC25B in terminally differentiated human germ cells.
Teng
Yen-Ni
YN
Department of Biotechnology, Chia Nan University of Pharmacy and Science, National Cheng Kung University, College of Medicine, Tainan, Taiwan.
Chung
Chia-Ling
CL
Lin
Yung-Ming
YM
Pan
Hsien-An
HA
Liao
Rui-Wen
RW
Kuo
Pao-Lin
PL
D000328:Adult; D053627:Asthenozoospermia / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008297:Male; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013094:Spermatozoa / Q000378:metabolism*; D014018:Tissue Distribution; D020687:cdc25 Phosphatases / Q000235:genetics* / Q000378:metabolism / Q000502:physiology
D018797:Cell Cycle Proteins; D007527:Isoenzymes; C517008:CDC25B protein, human; D020687:cdc25 Phosphatases