title: Studies of the central nervous system-derived CAD cell line, a suitable model for intraneuronal transport studies?
abstract: The CAD cell line is a variant of a CNS-derived Cath.a cell line established by targeted oncogenesis in transgenic mice. Cell differentiation of the cell line can be induced by "starvation," i.e., removal of serum from the culture medium (protein-free medium). The differentiated CAD cells extend long processes, which ultrastructurally contain abundant microtubules, intermediate filaments, and various synaptic vesicles/organelles in the varicose enlargements, thus resembling neurites. Histochemical studies demonstrated that the differentiated cells express a number of synaptic vesicle proteins, cytoskeletons, different neurotransmitter enzymes, neuropeptides, and glia proteins. The data suggest that the differentiated CAD cells may be a suitable model for studies of intraneuronal transport, recycling of receptors, and pharmacological investigations.
authors.affiliation: Department of Medical Chemistry and Cell Biology, Institute of Biomedicine, Göteborg University, Göteborg, Sweden. yongling.li@anatcell.gu.se
authors.forename: Yongling
authors.initials: Y
authors.lastname: Li
authors.forename: Linda Xiu-E
authors.initials: LX
authors.lastname: Hou
authors.forename: Annika
authors.initials: A
authors.lastname: Aktiv
authors.forename: Annica
authors.initials: A
authors.lastname: Dahlström
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line / Q000502:physiology*; D002490:Central Nervous System / Q000166:cytology*; D007382:Intermediate Filaments / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D008870:Microtubules / Q000378:metabolism; D008954:Models, Biological; D009419:Nerve Tissue Proteins / Q000378:metabolism
chemical_list: D009419:Nerve Tissue Proteins