Production of polyvinyl alcohol-degrading enzyme with Janthinobacterium sp. and its application in cotton fabric desizing.
A strain capable of using polyvinyl alcohol (PVA) as sole carbon source was isolated from soil samples of a textile factory. The 16S rDNA sequence analysis cell morphology, physiology and biochemistry showed that it belonged to Janthinobacterium sp. This is the first report to show that the screened Janthinobacterium sp. could degrade PVA. The optimum nutritional and environmental conditions for PVA-degrading enzyme production by Janthinobacterium sp. were investigated by single-factor tests. Under optimized nutritional and environmental condition in shake flasks, PVA-degrading enzyme reached 5.12 U/mL at 21 h. With PVA-degrading enzyme produced by Janthinobacterium sp. WSH04-01, 80% of PVA could be degraded from cotton fabrics in 3 h.
Du
Guocheng
G
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Southern Yangtze University, Wuxi, China.
Liu
Liming
L
Song
Zhaoxia
Z
Hua
Zhaozhe
Z
Zhu
Yang
Y
Chen
Jian
J
D000429:Alcohol Oxidoreductases / Q000737:chemistry* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D032561:Cotton Fiber; D011142:Polyvinyl Alcohol / Q000737:chemistry* / Q000378:metabolism*; D020560:Proteobacteria / Q000201:enzymology*; D013783:Textile Industry / Q000379:methods*
D011142:Polyvinyl Alcohol; D000429:Alcohol Oxidoreductases; C048312:polyvinyl-alcohol dehydrogenase (acceptor)