Interaction of Arcobacter spp. with human and porcine intestinal epithelial cells.
Little is known about the pathogenic mechanisms or potential virulence factors of Arcobacter spp. The aim of the study described here was to obtain more insights in the pathogenicity mechanisms of Arcobacter spp. by testing their ability to adhere to, invade and induce interleukin-8 expression in human Caco-2 and porcine IPI-2I cell lines. Eight Arcobacter strains were tested. Four strains were obtained from a culture collection, and represent the four Arcobacter spp. known to be associated with animals and humans. The other four strains were field isolates from the amniotic fluid of sows and from newborn piglets. All eight Arcobacter strains were able to adhere to both cell lines, and induced interleukin-8 production as early as 2 h after a 1h incubation period. This production was still increased 6 h postinfection. Differences in the cell association of the eight strains were obvious, with A. cibarius showing the highest adhesion ability. Invasion of intestinal epithelial cells was only observed for A. cryaerophilus strains. No correlation between invasiveness or strong adhesion of the tested strains and the level of interleukin-8 induction was observed.
Ho
Hoa T K
HT
Division of Public Health and Food Safety, Institute for Risk Assessment Sciences, Utrecht University, The Netherlands.
Lipman
Len J A
LJ
Hendriks
Henno G C J M
HG
Tooten
Peter C J
PC
Ultee
Ton
T
Gaastra
Wim
W
D000818:Animals; D020640:Arcobacter / Q000302:isolation & purification / Q000472:pathogenicity*; D001422:Bacterial Adhesion; D018938:Caco-2 Cells; D002460:Cell Line; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000382:microbiology*; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000276:immunology; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology* / Q000382:microbiology*; D034421:Sus scrofa; D013552:Swine
D016209:Interleukin-8