title: Implant surface coatings with bone sialoprotein, collagen, and fibronectin and their effects on cells derived from human maxillar bone.
abstract: The interaction between implant material and surrounding tissues is believed to play a fundamental role in implant success. Although bone sialoprotein (BSP) has been found to be osteoinductive when coated onto femoral implants, collagen and fibronectin are the most used compounds for preparation of pre-coated cell culture slides at present. In this study, the support of BSP-, collagen- and fibronectin-coated and non-coated implant material for the development of adult human maxillar bone in vitro was studied and compared. The expression of bone turnover markers like BSP and osteocalcin as well as osteonectin, transforming growth factor beta (TGF-beta) and CD90 during different time periods of cell cultivation (3, 5, 10, 15, 20 and 25 days) was visualized immunohistochemically. The distribution patterns of the cells were examined on a rough surface of the titanium-hydroxyapatite dental implant material TICER and on a total smooth surface of the technical implant material glimmer. Significantly different values were found for glimmer at the 15. and the 20. Div, exclusively, indicating that a smooth surface was more improved than a rough ceramic surface by pre-coatings. The White-test using rankings of the median values gave evidence for BSP-coatings at position 1 followed by collagen. Our experiments were designed to use very low concentrated BSP coating solution with the aim to reduce the healing time with a minimal effort and minimal risks for the patients.
authors.affiliation: Institute of Anatomy, Leipzig University, Liebigstr. 13, 04103 Leipzig, Germany. hilbigh@medizin.uni-leipzig.de
authors.forename: H
authors.initials: H
authors.lastname: Hilbig
authors.forename: M
authors.initials: M
authors.lastname: Kirsten
authors.forename: R
authors.initials: R
authors.lastname: Rupietta
authors.forename: H-L
authors.initials: HL
authors.lastname: Graf
authors.forename: S
authors.initials: S
authors.lastname: Thalhammer
authors.forename: S
authors.initials: S
authors.lastname: Strasser
authors.forename: F P
authors.initials: FP
authors.lastname: Armbruster
mesh_terms: D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D003094:Collagen / Q000494:pharmacology*; D005353:Fibronectins / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D058574:Integrin-Binding Sialoprotein; D008437:Maxilla / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D018625:Microscopy, Atomic Force; D015675:Osteocalcin / Q000378:metabolism; D015676:Osteonectin / Q000378:metabolism; D019736:Prostheses and Implants; D012795:Sialoglycoproteins / Q000494:pharmacology*; D013499:Surface Properties / Q000187:drug effects*; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D020099:Coated Materials, Biocompatible; D005353:Fibronectins; C546871:IBSP protein, human; D058574:Integrin-Binding Sialoprotein; D015676:Osteonectin; D012795:Sialoglycoproteins; D016212:Transforming Growth Factor beta; D015675:Osteocalcin; D003094:Collagen