title: Determination of N-acetyl retigabine in dog plasma by LC/MS/MS following off-line microElution 96-well solid phase extraction.
abstract: A high throughput off-line microElution 96-well solid phase extraction (SPE) followed by liquid chromatography with tandem mass spectrometry (LC/MS/MS) quantification for the determination of N-acetyl retigabine in dog plasma has been developed and validated. The method involves the use of microElution 96-well SPE for the simultaneous extraction of N-acetyl retigabine and rapid removal of its N-glucuronide metabolite that has shown to be problematic due to its instability using other clean-up methods. The microElution SPE technology eliminates the need for post-extraction solvent evaporation and greatly reduces sample preparation time consequently improving assay efficiency.
authors.affiliation: Valeant Research & Development, 3300 Hyland Avenue, Costa Mesa, CA 92626, USA. wbu@anacor.com
authors.forename: Wei
authors.initials: W
authors.lastname: Bu
authors.forename: Mai
authors.initials: M
authors.lastname: Nguyen
authors.forename: Christine
authors.initials: C
authors.lastname: Xu
authors.forename: Chin-Chung
authors.initials: CC
authors.lastname: Lin
authors.forename: Li-Tain
authors.initials: LT
authors.lastname: Yeh
authors.forename: Virginia
authors.initials: V
authors.lastname: Borges
mesh_terms: D000818:Animals; D002138:Calibration; D002219:Carbamates / Q000097:blood* / Q000493:pharmacokinetics / Q000633:toxicity; D002853:Chromatography, Liquid / Q000379:methods*; D004285:Dogs; D005260:Female; D008297:Male; D010655:Phenylenediamines / Q000097:blood* / Q000493:pharmacokinetics / Q000633:toxicity; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D002219:Carbamates; D010655:Phenylenediamines; C101866:ezogabine