title: Effect of panel-reactive antibody in predicting crossmatch selection of cadaveric kidney recipients.
abstract: OBJECTIVE
  The influence of panel-reactive antibody level (%PRA) on crossmatch results was evaluated among 866 patients on the waiting list for cadaveric renal allografting from January 2001 to August 2005. We evaluated the results for 124 potential donors for a kidney, including 2008 crossmatches. Four hundred eighteen patients were tested against only 1 donor.
  
  
  METHODS
  Serum samples were screened for anti-HLA antibodies using immunoglobulin (Ig)G enzyme-linked immunosorbent assay (ELISA) PRA kit and the %PRA of the most reactive sample (peak) was used for patient stratification, according to sensitization level. Crossmatches were performed on fresh donor T lymphocytes from peripheral lymph nodes, using classical and anti-human-globulin enhanced complement-dependent cytotoxicity (CDC-T) methods. The tests were performed using peak and current patient sera before and after dithiothreitol treatment. The crossmatch was assumed to be negative when no reactivity was observed in all tests.
  
  
  RESULTS
  The incidences of positive crossmatch were as follows: 72.3%, 14.6%, and 7.2%, among patients with PRA >50%, PRA </=50%, and PRA = 0%, respectively. A negative crossmatch was observed in 6.5% (4 of 62) of the tests among 100% PRA patients.
  
  
  CONCLUSIONS
  Although %PRA level reflects the HLA sensitization of the patient, IgG ELISA PRA and CDC-T crossmatch may not reflect the same antibodies, and both results must be considered in a transplantation decision.
authors.affiliation: Campinas State University, Hematology and Hemotherapy Center, Campinas State University, Campinas, São Paulo, Brazil. slieber@osite.com.br
authors.forename: S R
authors.initials: SR
authors.lastname: Lieber
authors.forename: F V T
authors.initials: FV
authors.lastname: Perez
authors.forename: M R
authors.initials: MR
authors.lastname: Tabossi
authors.forename: L B L
authors.initials: LB
authors.lastname: Persoli
authors.forename: S B D
authors.initials: SB
authors.lastname: Marques
authors.forename: M
authors.initials: M
authors.lastname: Mazzali
authors.forename: G
authors.initials: G
authors.lastname: Alves-Filho
authors.forename: C A
authors.initials: CA
authors.lastname: de Souza
mesh_terms: D000017:ABO Blood-Group System / Q000276:immunology; D002102:Cadaver; D006084:Graft Rejection / Q000276:immunology; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D007518:Isoantibodies / Q000276:immunology*; D016030:Kidney Transplantation / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D014019:Tissue Donors; D014850:Waiting Lists
chemical_list: D000017:ABO Blood-Group System; D007518:Isoantibodies