Leucocyte antibodies in blood donors and a look back on recipients of their blood components.
OBJECTIVE
The role of leucocyte antibodies in donors is poorly understood in pathogenesis of transfusion-related acute lung injury (TRALI). We examined antibodies in donors and traced recipients transfused with their blood components.


METHODS
Antibodies were examined in 1043 donors by five methods, look back performed in 26 recipients.


RESULTS
Anti-human leucocyte antigen detected by enzyme-linked immunosorbent assay in 9.8% women but none in men. Specificities identified using FlowPRA, antibodies detected after several months. TRALI reported in one recipient from immunized donor. In 11 of 26 recipients without TRALI, cognate antigens were identified.


CONCLUSIONS
Detection of antibodies in donors cannot predict TRALI, even in recipients with cognate antigen(s).
Maślanka
K
K
Institute of Haematology and Blood Transfusion, Warsaw, Poland.
Michur
H
H
Zupańska
B
B
Uhrynowska
M
M
Nowak
J
J
D000328:Adult; D001782:Blood Donors; D005260:Female; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D007518:Isoantibodies / Q000009:adverse effects / Q000097:blood; D017708:Leukocyte Transfusion / Q000009:adverse effects*; D008297:Male; D008875:Middle Aged; D010298:Parity / Q000276:immunology*; D011247:Pregnancy; D012128:Respiratory Distress Syndrome / Q000276:immunology* / Q000517:prevention & control
D006680:HLA Antigens; D007518:Isoantibodies