title: Contribution of environmental mycobacteria to false-positive serum ELISA results for paratuberculosis.
abstract: OBJECTIVE
  To evaluate the effect of exposure to environmental mycobacteria on results of 2 commercial ELISAs for paratuberculosis in cattle.
  
  
  METHODS
  Experimental trial.
  
  
  METHODS
  19 weaned crossbred beef calves.
  
  
  METHODS
  Calves were inoculated SC with 1 of 5 mycobacterial isolates (3 calves/isolate) derived from herds with high proportions of false-positive serologic reactions for paratuberculosis, Mycobacterium avium subsp paratuberculosis (MAP; positive control inoculum; 2 calves), or mineral oil (negative control inoculum; 2 calves). Sera were assessed at intervals by use of 2 ELISAs (A and B) for paratuberculosis in cattle, and all calves underwent tuberculosis testing at the end of the study.
  
  
  RESULTS
  Neither mineral oil-inoculated calf had positive results with either ELISA during the study. Both MAP-inoculated calves were identified as seropositive via ELISA-A, and 1 calf was identified as seropositive via ELISA-B. By use of ELISA-A, > or = 1 false-positive reaction over time was detected in 2, 3, 3, and 1 of the 3 calves injected with Mycobacterium avium, Mycobacterium intracellulare, Mycobacterium scrofulaceum, or Mycobacterium terrae, respectively. By use of ELISA-B, only M scrofulaceum induced false-positive reactions (2/3 calves). Calves that had at least 1 positive ELISA-A result were more likely to be classified as suspect reactors via the caudal fold tuberculosis test.
  
  
  CONCLUSIONS
  False-positive serologic reactions may occur during use of commercially available ELISAs for paratuberculosis in calves experimentally exposed to environmental mycobacteria; naturally occurring exposures with these mycobacteria may represent a cause for high proportions of false-positive serologic reactions for paratuberculosis in some cattle herds.
authors.affiliation: Department of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA.
authors.forename: Jason B
authors.initials: JB
authors.lastname: Osterstock
authors.forename: Geoffrey T
authors.initials: GT
authors.lastname: Fosgate
authors.forename: Bo
authors.initials: B
authors.lastname: Norby
authors.forename: Elizabeth J B
authors.initials: EJ
authors.lastname: Manning
authors.forename: Michael T
authors.initials: MT
authors.lastname: Collins
authors.forename: Allen J
authors.initials: AJ
authors.lastname: Roussel
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000453:epidemiology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards / Q000662:veterinary*; D005189:False Positive Reactions; D005260:Female; D008297:Male; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology*; D010283:Paratuberculosis / Q000175:diagnosis / Q000453:epidemiology*; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies; D013045:Species Specificity; D014374:Tuberculin Test / Q000662:veterinary
chemical_list: D000907:Antibodies, Bacterial