Auxin pulse treatment holds the potential to enhance efficiency and practicability of somatic embryogenesis in potato.
The objective of the current study was to simplify existing somatic embryogenesis systems in potato (Solanum tuberosum L.) cv. Desiree. The project targeted the agar-based induction phase of the potato somatic embryogenesis process as the key area for improvement. Experiments were established to ascertain the effect of a 2,4-D (2,4 dichlorophenoxyacetic acid) pulse, applied to the primary internodal section explant source and its subsequent effect on embryo induction. Parameters tested were the duration of the auxin pulse in a range from 0 to 300 min, and the concentrations of 2,4-D applied, in a range from 0 to 5,120 microM. The mean number of somatic embryos formed per explant was recorded after 4 and 8 weeks culture. Our findings indicated that the somatic embryogenesis in potato internodal segments could be evoked by an auxin (2,4-D) pulse treatment over a wide concentration and duration range. The results further suggested that a simple 20 microM 2,4-D pulse treatment could replace a lengthy 2 week induction phase in potato somatic embryogenesis and thus improve the system's practicability for wider uptake.
Sharma
Sanjeev Kumar
SK
Genetics Programme, Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, UK.
Bryan
Glenn J
GJ
Millam
Steve
S
D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D007210:Indoleacetic Acids / Q000494:pharmacology*; D011198:Solanum tuberosum / Q000166:cytology / Q000187:drug effects / Q000196:embryology*; D013997:Time Factors; D046509:Tissue Culture Techniques / Q000379:methods*
D007210:Indoleacetic Acids; D015084:2,4-Dichlorophenoxyacetic Acid