[Experimental study of acute lung injury induced by glutamate in vivo].
OBJECTIVE
To investigate the possible injury induced by glutamate in the lung.


METHODS
The lung wet weight/body weight (LW/BW), lung wet/dry weight (W/D), the content of cells and the total protein (TP) in bronchoalveolar lavage fluid (BALF) were determined together with the micromorphology observation.


RESULTS
(1) The LW/BW, W/D, the content of white blood cells, red blood cells and TP in BALF increased in a dose dependent manner 2 hours after the administration of the glutamate (0.50 - 0.75 g/kg). (2) Examination of histological sections showed the presence of lung inflammation charactered by neutrophils recruitment 2 hours after the glutamate administration. (3) The increase of W/D caused by glutamate (0.50 g/kg) was nearly abolished by pre-treatment with MK801 (a specific blocker of NMDA receptor, 0.1 mg/kg) for 30 minutes (P<0.05).


CONCLUSIONS
Glutamate can cause the acute lung injury through the activation of NMDA receptor in vivo.
Shen
Li
L
Department of Physiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Luo
Zi-Qiang
ZQ
Han
Jian-Zhong
JZ
Yue
Shao-Jie
SJ
Qin
Xiao-Qun
XQ
Li
Chen
C
Liu
Hui-Jun
HJ
D000208:Acute Disease; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D016291:Dizocilpine Maleate / Q000494:pharmacology; D004906:Erythrocyte Count; D018698:Glutamic Acid / Q000008:administration & dosage / Q000633:toxicity*; D007958:Leukocyte Count; D008171:Lung Diseases / Q000139:chemically induced* / Q000378:metabolism / Q000517:prevention & control; D008297:Male; D051379:Mice; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors / Q000378:metabolism
D016194:Receptors, N-Methyl-D-Aspartate; D018698:Glutamic Acid; D016291:Dizocilpine Maleate