title: Superoxide dismutase and catalase activities in the seminal plasma of normozoospermic and asthenozoospermic Beagles.
abstract: We measured the blood plasma testosterone (T) levels and superoxide dismutase (SOD) and catalase activities in the seminal plasma of the ejaculates of 5 normal (2-5 years old) and 5 asthenozoospermic (AZ-) (3-5 years old) Beagles. Sperm ejaculated by AZ-dogs was incubated for 3 hr in Eagle's MEM only (controls) or Eagle's MEM containing 100 units/ml of SOD or catalase. Sperm motility was examined during incubation. The mean (+/- SE) plasma T level of the AZ-dogs (1.2 +/- 0.2 ng/ml) was significantly lower than in the normal dogs (2.5 +/- 0.2 ng/ml) (P<0.005). The mean (+/- SE) seminal plasma SOD and catalase activities (18.8 +/- 1.9 and 0.5 +/- 0.1 unit/g protein, respectively) were significantly lower in the AZ-dogs than in the normal dog (43.3 +/- 2.5 and 2.2 +/- 0.4 unit/g protein, respectively) (P<0.001 and 0.01, respectively). The motility of sperm incubated in Eagle's MEM containing SOD or catalase was significantly higher than that of control sperm incubated in only Eagle's MEM after 2 or 3 hr of incubation (P<0.05). The results of this study indicate that poor T secretion by the testes and low antioxidant enzyme activities are related to AZ in the dog.
authors.affiliation: Department of Reproduction, Nippon Veterinary and Life Science University, Musashino-shi, Tokyo, Japan.
authors.forename: Eiichi
authors.initials: E
authors.lastname: Kawakami
authors.forename: Ariko
authors.initials: A
authors.lastname: Takemura
authors.forename: Masaki
authors.initials: M
authors.lastname: Sakuma
authors.forename: Mai
authors.initials: M
authors.lastname: Takano
authors.forename: Taichi
authors.initials: T
authors.lastname: Hirano
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Hori
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Tsutsui
mesh_terms: D000818:Animals; D053627:Asthenozoospermia / Q000097:blood / Q000201:enzymology / Q000473:pathology / Q000662:veterinary*; D002374:Catalase / Q000378:metabolism*; D004283:Dog Diseases / Q000097:blood / Q000201:enzymology* / Q000473:pathology; D004285:Dogs; D008297:Male; D012661:Semen / Q000201:enzymology*; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000002:abnormalities; D013482:Superoxide Dismutase / Q000378:metabolism*; D013739:Testosterone / Q000097:blood
chemical_list: D013739:Testosterone; D002374:Catalase; D013482:Superoxide Dismutase