Comparison of a new gold-immunochromatographic assay for the detection of antibodies against avian influenza virus with hemagglutination inhibition and agar gel immunodiffusion assays.
A gold-immunochromatographic test-strip kit is used for the detection of IgG antibodies against the nucleocapsid protein of Avian Influenza Virus (AIV). Compared with the "gold standard", i.e. hemagglutination inhibition (HI) and agar gel immunodiffusion (AGID) assays, the gold-immunochromatographic test strip has many advantages, such as high specificity, high sensitivity, convenience, is rapid and has low cost. The gold-immunochromatographic test strip provides a unique tool for the on-site surveillance and diagnosis of Avian Influenza.
Peng
DaPeng
D
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Hu
SiShun
S
Hua
Yan
Y
Xiao
YunCai
Y
Li
Zili
Z
Wang
XiLiang
X
Bi
DingRen
D
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002645:Chickens; D017979:Gold Colloid / Q000737:chemistry; D006385:Hemagglutination Inhibition Tests / Q000379:methods / Q000662:veterinary; D005779:Immunodiffusion / Q000379:methods / Q000662:veterinary; D007074:Immunoglobulin G / Q000097:blood; D007150:Immunohistochemistry / Q000379:methods / Q000592:standards / Q000662:veterinary*; D009980:Influenza A virus / Q000276:immunology* / Q000302:isolation & purification; D005585:Influenza in Birds / Q000175:diagnosis / Q000276:immunology* / Q000821:virology; D019590:Nucleocapsid Proteins / Q000276:immunology; D011934:Reagent Strips; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013047:Specific Pathogen-Free Organisms
D000914:Antibodies, Viral; D017979:Gold Colloid; D007074:Immunoglobulin G; D019590:Nucleocapsid Proteins; D011934:Reagent Strips