title: [Rat dental papilla cell culture with nanometer-HAP in vitro].
abstract: OBJECTIVE
  To determine the effects of nano-hydroxyapatite(nano-HAP)on the proliferation and activity of rat dental papilla cells(RDPCs)in vitro, and to evaluate the feasibility of using nano-hydroxyapatite(nano-HAP)as dental papilla cell scaffold in dental tissue engineering.
  
  
  METHODS
  RDPCs cultured with the porous nano-HAP in vitro served as the experimental group, and the routine culture of RDPCs in flasks served as the control. Scanning electronic microscope was used to observe the growth and adherence of the RDPCs to nano-HAP. Cell proliferation, cellular protein content, and alkaline phosphatase(ALP) were detected to assess the cellular activities.
  
  
  RESULTS
  RDPCs proliferated well, and adhered to the outer and inner surface of the nano-HAP scaffold. Compared with the control group, cells in the experimental group presented higher proliferation on 6 d and 8 d and higher cellular protein content on 6 d and 9 d. No significant difference was detected in the ALP activity in the 2 groups.
  
  
  CONCLUSIONS
  RDPCs seeded into nano-HAP grow better and have more vigorous cellular activity, suggesting that nano-HAP has excellent biocompatibility with dental papilla cells, and it can serve as a promising scaffold for dental tissue engineering.
authors.affiliation: Department of Stomatology, Xiangya Hospital, Central South University, Changsha 410078, China.
authors.forename: Chang-Yun
authors.initials: CY
authors.lastname: Fang
authors.forename: Ying
authors.initials: Y
authors.lastname: Cao
authors.forename: Yu
authors.initials: Y
authors.lastname: Xia
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Zhang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Su
authors.forename: Hui-Li
authors.initials: HL
authors.lastname: Li
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001672:Biocompatible Materials / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003771:Dental Papilla / Q000166:cytology* / Q000648:ultrastructure; D017886:Durapatite / Q000494:pharmacology*; D008854:Microscopy, Electron; D053758:Nanoparticles; D036103:Nanotechnology; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D017886:Durapatite