[Research on Sailong boneextracts on proliferation and apoptosis of osteoblast cells].
OBJECTIVE
To investigate the metabolic regulation and apoptosis of Sailong bone extracts on rat osteoblast cells in vitro.


METHODS
Sailong bone fat-soluble extract, Sailong bone ethanol extract and Sailong bone aqueous extract were extracted with super critical fluid extraction (SCFE) , and Sailong bone boiling water component was extracted with distilled water directly. MTT assay was applied to determine the proliferation of the cell promoted by four Sailong bone extracts and PAS assay for the aqueous proportion of the cell at different doses.


RESULTS
Sailong bone fat-soluble and aqueous extract (each 10 mg x mL (-1)) could significantly improve the proliferation of rat osteoblast cells ROS 17/2. 8 (P < 0. 01). Compared with the blank, the proportion of xub-G, of the different extracts from Sailong bone is reduce evidently. The result have shown the extracts from Sailong bone could reduce the rate of aqueous of cell and could suspend the aqueous.


CONCLUSIONS
Sailong bone can promoting the proliferation, degrading the rate of the apoptosis and delay the development of osteoblast to be the substitute of the bone of tiger as a Chinese materia medica.
Pan
Ming
M
Life Science School, Sichuan University, Chengdu 610064, China. panming106@163.com
Shi
Jue
J
Luo
Xia
X
Hou
Ruo-tong
RT
Yu
Meng-yao
MY
Yang
Zhi-rong
ZR
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001842:Bone and Bones / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D008419:Materia Medica / Q000302:isolation & purification / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D012377:Rodentia; D013997:Time Factors
D008419:Materia Medica