title: Effect of a diterpenoid from Salvia cinnabarina on arterial blood pressure in rats.
abstract: The effect of a diterpenoid isolated from Salvia cinnabarina, 3,4-seicosopimar-4(18),7,15-triene-3-oic acid (SCB), on arterial blood pressure was evaluated in anaesthetized rats. Male Wistar rats, anaesthetized with urethane (sol. 10% p/v; 10 mL/kg), underwent surgery for continuous monitoring of arterial blood pressure. After preliminary experiments to evaluate the dose response (3, 10 and 30 mg/kg i.v.) of SCB, a dose of 3 mg/kg was chosen for all successive experiments. On different groups of rats treated with the ganglion-blocking agent chlorisondamine (2.5 mg/kg i.p.) the effect of SCB (3 mg/kg i.v.) was evaluated before and following an infusion of the nitric oxide synthase inhibitor L-NAME (0.3 mg/kg/min i.v.). Intravenous administration of SCB at doses of 3, 10 and 30 mg/kg led to a fall in mean arterial blood pressure (MABP) of 14.75 +/- 1.44 mmHg, 36.60 +/- 31.40 mmHg and 31.40 +/- 6.28 mmHg, respectively (n = 4-5), that was not modified by treatment of the rat with chlorisondamine nor with L-NAME. The results demonstrate a hypotensive effect of SCB - due to a peripheral mechanism but independent of endothelial nitric oxide release.
authors.affiliation: Dipartimento di Farmacologia Sperimentale, University of Naples Federico II, via D. Montesano 49, 80131 Napoli, Italy.
authors.forename: Alessio
authors.initials: A
authors.lastname: Alfieri
authors.forename: Francesco
authors.initials: F
authors.lastname: Maione
authors.forename: Angela
authors.initials: A
authors.lastname: Bisio
authors.forename: Giovanni
authors.initials: G
authors.lastname: Romussi
authors.forename: Nicola
authors.initials: N
authors.lastname: Mascolo
authors.forename: Carla
authors.initials: C
authors.lastname: Cicala
mesh_terms: D000818:Animals; D000959:Antihypertensive Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D001794:Blood Pressure / Q000187:drug effects*; D004224:Diterpenes / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D007275:Injections, Intravenous; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D027544:Salvia
chemical_list: D000959:Antihypertensive Agents; D004224:Diterpenes; D010936:Plant Extracts