Immuno-stimulating properties of diosgenyl saponins isolated from Paris polyphylla.
The effects of three diosgenyl saponins isolated from Paris polyphylla on the immuno-stimulating activity in relation to phagocytosis, respiratory burst, and nitric oxide production in mouse macrophage cells RAW 264.7 have been investigated. Our results showed that all three diosgenyl saponins significantly enhanced phagocytic activity that increased with the concentration of saponins to reach a maximum, and then tended to decrease with higher concentrations. Saponins with sugar moiety directly induced respiratory burst response in RAW 264.7 cells that increased with the concentrations and reached a maximum, then decreased with higher concentrations after 2-h incubations, however, diosgenin had no PMA-triggered respiratory burst response. Treatment of RAW 264.7 cells with saponins with sugar moiety for 24-h caused a significant increase in the production of nitric oxide, while diosgenin had no effect at all. Consequently, relationship between molecular structures of three diosgenyl saponins and their immunomodulatory activities was discussed, and a possible mechanism of immuno-stimulating function of diosgenyl saponins was accordingly explored.
Zhang
Xiu-feng
XF
State Key laboratory for SCUSS, Institute of Chemistry, The Chinese Academy of Sciences, Beijing 100080, China.
Cui
Yan
Y
Huang
Jia-jun
JJ
Zhang
Ya-zhou
YZ
Nie
Zhou
Z
Wang
Lan-fen
LF
Yan
Bao-zhen
BZ
Tang
Ya-lin
YL
Liu
Yang
Y
D000818:Animals; D002460:Cell Line; D002626:Chemistry, Pharmaceutical / Q000379:methods; D015195:Drug Design; D019668:Liliaceae / Q000737:chemistry*; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D008956:Models, Chemical; D015394:Molecular Structure; D009569:Nitric Oxide / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D016897:Respiratory Burst / Q000187:drug effects; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification*
D010936:Plant Extracts; D012503:Saponins; D009569:Nitric Oxide