title: Molecular detection of Chlamydia trachomatis and HPV infections in cervical samples with normal and abnormal cytopathological findings.
abstract: It has been suggested that Chlamydia trachomatis (CT) and human papillomaviruses (HPV) co-infection could contribute to development of intraepithelial lesions. In this study, HPV and CT-DNA were investigated in 250 cervicovaginal samples of patients from Minas Gerais, Brazil. The cytological analysis revealed that 70% of samples (175) were negative, 5.2% (13) presented atypical squamous or glandular cells of undetermined significance (ASCUS/AGUS), 12.4% (31) presented low-grade squamous intraepithelial lesion (LSIL), 10.8% (27) high-grade squamous intraepithelial lesion (HSIL), and 1.6% (4) invasive carcinoma. HPV-DNA and HPV/CT co-infection was observed in 40% (100/250) and in 5.2% (13/250) of samples, respectively. Among the positive cytological samples, HPV-DNA was detected in 73.3% and CT-DNA in 9.33% and in 13%, if only the HPV positive samples were considered. The highest co-infection rate (15.4%) was observed among ASCUS/AGUS samples. Although a significant association was found for HPV infection and the precursor lesions of cervical cancer, it was not possible to establish a significant association between these lesions and CT or HPV/CT co-infection.
authors.affiliation: Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, CEP 31270 901 Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Francisco Danilo Ferreira
authors.initials: FD
authors.lastname: de Paula
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Fernandes
authors.forename: Bianca Bianco do
authors.initials: BB
authors.lastname: Carmo
authors.forename: Daniela Cristina Duarte
authors.initials: DC
authors.lastname: Vieira
authors.forename: Miriam Santos
authors.initials: MS
authors.lastname: Dutra
authors.forename: Caleb Guedes Miranda dos
authors.initials: CG
authors.lastname: Santos
authors.forename: Maria do Carmo M
authors.initials: Mdo C
authors.lastname: Souza
authors.forename: Túlio César Alvim
authors.initials: TC
authors.lastname: Andrade
authors.forename: Annamaria Ravara
authors.initials: AR
authors.lastname: Vago
authors.forename: Paula Avila
authors.initials: PA
authors.lastname: Fernandes
mesh_terms: D000328:Adult; D002584:Cervix Uteri / Q000473:pathology* / Q000821:virology*; D002690:Chlamydia Infections / Q000150:complications* / Q000453:epidemiology; D002692:Chlamydia trachomatis / Q000302:isolation & purification; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000150:complications* / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D011230:Precancerous Conditions / Q000821:virology; D014412:Tumor Virus Infections / Q000150:complications / Q000453:epidemiology*; D002583:Uterine Cervical Neoplasms / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000821:virology
chemical_list: D004279:DNA, Viral