Convenient synthesis of maleimido-derivatized lanthanide(III) chelates and their use in mercapto group conjugation.
Simple synthesis of luminescent europium(III) and terbium(III) chelates tethered to a maleimido function (7, 8) is described. The method is based on the following: (i) synthesis of protected ligands tethered to a maleimido function and their purification on silica gel; (ii) deprotection by acidolysis; (iii) conversion of the deprotected ligands to the corresponding lanthanide(III) chelates by passing them through a column of strong cation exchange resin loaded with the appropriate lanthanide(III) ions. According to this procedure, large quantities of mercapto-selective biomolecule-labeling reactants of high purity can be prepared.
Hovinen
Jari
J
PerkinElmer Life and Analytical Sciences, Turku Site, Turku, Finland. jari.hovinen@perkinelmer.com
D002614:Chelating Agents / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005063:Europium / Q000737:chemistry; D028581:Lanthanoid Series Elements / Q000138:chemical synthesis* / Q000737:chemistry; D008024:Ligands; D049408:Luminescent Agents / Q000737:chemistry; D053758:Nanoparticles; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry*; D011506:Proteins / Q000138:chemical synthesis / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry / Q000378:metabolism*; D013725:Terbium / Q000737:chemistry
D002614:Chelating Agents; D028581:Lanthanoid Series Elements; D008024:Ligands; D049408:Luminescent Agents; D009842:Oligopeptides; D011506:Proteins; D013438:Sulfhydryl Compounds; D013725:Terbium; D005063:Europium