title: Dynamic changes in CREB phosphorylation and neuroadaptive gene expression in area V1 of adult monkeys after monocular enucleation.
abstract: Our understanding of the molecular events that emerge after change in sensory input remains elusive, especially with regard to mature area V1. Here, we characterized P-CREB expression in area V1 of monkeys at multiple time-points after monocular enucleation (ME) to assess the possible contribution of CREB in visually deprived neocortex. Immunoblot assays and immunostainings showed that P-CREB is dynamically regulated in adult area V1, reaching a peak level between 5 and 30 days after ME, and becoming reduced at the 90-day post-ME time-point. This striking temporal increase in P-CREB level was paralleled by a concomitant increase of two CREB-regulated pro-survival effectors, namely Bcl-2 and Bcl-w. We present our results in the context of recent advances about adult visual neocortex and propose that ME induces a multifaceted CREB-mediated response that favors intrinsic stability of neurons and facilitates mature cortical networks to reorganize over a prolonged period.
authors.affiliation: Department of Psychology, McGill University, 1205 Dr. Penfield Avenue, Montréal, Québec, Canada H3A 1B1. jasmin@ego.psych.mcgill.ca
authors.forename: Jasmin
authors.initials: J
authors.lastname: Lalonde
authors.forename: Avi
authors.initials: A
authors.lastname: Chaudhuri
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology*; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D001766:Blindness / Q000378:metabolism / Q000503:physiopathology*; D002522:Chlorocebus aethiops; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics* / Q000378:metabolism*; D015353:Eye Enucleation; D015870:Gene Expression; D015151:Immunoblotting; D016328:NF-kappa B / Q000378:metabolism; D009473:Neuronal Plasticity / Q000502:physiology; D010766:Phosphorylation; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D014793:Visual Cortex / Q000378:metabolism / Q000502:physiology*
chemical_list: D051017:Apoptosis Regulatory Proteins; D017362:Cyclic AMP Response Element-Binding Protein; D016328:NF-kappa B; D019253:Proto-Oncogene Proteins c-bcl-2