title: On-line liquid chromatography-electrospray ionisation mass spectrometry for kappa-carrageenan oligosaccharides with a porous graphitic carbon column.
abstract: Enzymatically digested oligosaccharides of kappa-carrageenans were separated on a porous graphitic carbon (PGC) column and characterised on-line by electrospray ionisation mass spectrometry (ESI-MS). Two different developing ions were applied. Among them ammonium hydrogencarbonate showed more eluting power as it should on normal anion-exchange stationary phases. The oligosaccharides were detected by ESI-MS as fully deprotonated oligosaccharides.
authors.affiliation: Institut de Chimie Organique et Analytique (ICOA), UMR CNRS 6005, Université d'Orléans, B.P. 6759, 45067 Orléans Cedex 2, France.
authors.forename: Aristotelis
authors.initials: A
authors.lastname: Antonopoulos
authors.forename: Patrick
authors.initials: P
authors.lastname: Favetta
authors.forename: William
authors.initials: W
authors.lastname: Helbert
authors.forename: Michel
authors.initials: M
authors.lastname: Lafosse
mesh_terms: D000837:Anion Exchange Resins; D002244:Carbon / Q000737:chemistry*; D002351:Carrageenan / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D006108:Graphite / Q000737:chemistry*; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry; D016062:Porosity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D000837:Anion Exchange Resins; D009844:Oligosaccharides; D002244:Carbon; D006108:Graphite; D002351:Carrageenan