title: Identification of in vivo-expressed immunogenic proteins by serological proteome analysis of the Bacillus anthracis secretome.
abstract: In a previous comparative proteomic study of Bacillus anthracis examining the influence of the virulence plasmids and of various growth conditions on the composition of the bacterial secretome, we identified 64 abundantly expressed proteins (T. Chitlaru, O. Gat, Y. Gozlan, N. Ariel, and A. Shafferman, J. Bacteriol. 188:3551-3571, 2006). Using a battery of sera from B. anthracis-infected animals, in the present study we demonstrated that 49 of these proteins are immunogenic. Thirty-eight B. anthracis immunogens are documented in this study for the first time. The relative immunogenicities of the 49 secreted proteins appear to span a >10,000-fold range. The proteins eliciting the highest humoral response in the course of infection include, in addition to the well-established immunogens protective antigen (PA), Sap, and EA1, GroEL (BA0267), AhpC (BA0345), MntA (BA3189), HtrA (BA3660), 2,3-cyclic nucleotide diesterase (BA4346), collagen adhesin (BAS5205), an alanine amidase (BA0898), and an endopeptidase (BA1952), as well as three proteins having unknown functions (BA0796, BA0799, and BA0307). Of these 14 highly potent secreted immunogens, 11 are known to be associated with virulence and pathogenicity in B. anthracis or in other bacterial pathogens. Combining the results reported here with the results of a similar study of the membranal proteome of B. anthracis (T. Chitlaru, N. Ariel, A. Zvi, M. Lion, B. Velan, A. Shafferman, and E. Elhanany, Proteomics 4:677-691, 2004) and the results obtained in a functional genomic search for immunogens (O. Gat, H. Grosfeld, N. Ariel, I. Inbar, G. Zaide, Y. Broder, A. Zvi, T. Chitlaru, Z. Altboum, D. Stein, S. Cohen, and A. Shafferman, Infect. Immun. 74:3987-4001, 2006), we generated a list of 84 in vivo-expressed immunogens for future evaluation for vaccine development, diagnostics, and/or therapeutic intervention. In a preliminary study, the efficacies of eight immunogens following DNA immunization of guinea pigs were compared to the efficacy of a PA DNA vaccine. All eight immunogens induced specific high antibody titers comparable to the titers elicited by PA; however, unlike PA, none of them provided protection against a lethal challenge (50 50% lethal doses) of virulent B. anthracis strain Vollum spores.
authors.affiliation: Israel Institute for Biological Research, P.O. Box 19, Ness-Ziona 74100, Israel.
authors.forename: Theodor
authors.initials: T
authors.lastname: Chitlaru
authors.forename: Orit
authors.initials: O
authors.lastname: Gat
authors.forename: Haim
authors.initials: H
authors.lastname: Grosfeld
authors.forename: Itzhak
authors.initials: I
authors.lastname: Inbar
authors.forename: Yael
authors.initials: Y
authors.lastname: Gozlan
authors.forename: Avigdor
authors.initials: A
authors.lastname: Shafferman
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000302:isolation & purification* / Q000378:metabolism; D001408:Bacillus anthracis / Q000737:chemistry*; D006168:Guinea Pigs; D020543:Proteome / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D012698:Serologic Tests
chemical_list: D000942:Antigens, Bacterial; D020543:Proteome