title: Non-invasive measurement of the morphology and physiology of oral mucosa by use of optical spectroscopy.
abstract: Differential path-length spectroscopy (DPS) was used to non-invasively determine the superficial optical properties of oral mucosa in vivo. DPS yields information on physiological parameters such as the mucosal blood content, the microvascular blood oxygenation and the average micro-vessel diameter as well as on morphological parameters such as the scattering slope and scattering amplitude. DPS measurements were made on normal and cancerous oral mucosa using a novel fiber-optic probe, and were correlated to the histological outcome of punch biopsies taken from the same location. Our data shows that the mucosa of oral squamous cell carcinoma is characterised by a significant decrease in microvascular oxygenation and increase in mucosal blood content compared to normal oral mucosa as well as a significant decrease in scattering amplitude and increase in scattering slope.
authors.affiliation: Center for Optical Diagnostics and Therapy, Department of Radiation Oncology, Erasmus Medical Centre, P.O. Box 2040, 3000 CA Rotterdam, The Netherlands.
authors.forename: A
authors.initials: A
authors.lastname: Amelink
authors.forename: O P
authors.initials: OP
authors.lastname: Kaspers
authors.forename: H J C M
authors.initials: HJ
authors.lastname: Sterenborg
authors.forename: J E
authors.initials: JE
authors.lastname: van der Wal
authors.forename: J L N
authors.initials: JL
authors.lastname: Roodenburg
authors.forename: M J H
authors.initials: MJ
authors.lastname: Witjes
mesh_terms: D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000098:blood supply / Q000473:pathology*; D005260:Female; D005336:Fiber Optic Technology / Q000295:instrumentation; D006801:Humans; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000033:anatomy & histology / Q000473:pathology*; D009062:Mouth Neoplasms / Q000098:blood supply / Q000473:pathology*; D055100:Optical Fibers; D010100:Oxygen / Q000097:blood; D013057:Spectrum Analysis / Q000295:instrumentation / Q000379:methods
chemical_list: D010100:Oxygen