[Detection of erucic acid and glucosinolate in intact rapeseed by near-infrared diffuse reflectance spectroscopy].
With the rapid development of transgenic food, more and more transgenic food has been pouring into the market, raising great concern about transgenic food' s edible safety. To analyze the content of erucic acid and glucosinolate in transgenic rapeseed and its parents, all the seeds were scanned intact by continuous wave of near infrared diffuse reflectance spectrometry ranging from 12 000 to 4 000 cm(-1) with a resolution of 4 cm(-1) and 64 times of scanning. Bruker OPUS software package was applied for quantification, while the results were compared with the standard methods. The results showed that the method of NIRS was very precise, which proved that infrared diffuse reflectance spectroscopy can be applied to detect the toxins in transgenic food. On the other hand, the results also showed that the content of erucic acid in transgenic rapeseeds is 0. 5-1. 0 times
Riu
Yu-kui
YK
Laboratory of Food Technology of China Agricultural University, Beijing 100083, China.
Huang
Kun-lun
KL
Wang
Wei-min
WM
Guo
Jing
J
Jin
Yin-hua
YH
Luo
Yun-bo
YB
D029741:Brassica rapa / Q000737:chemistry*; D004883:Erucic Acids / Q000032:analysis*; D005961:Glucosinolates / Q000032:analysis*; D019265:Spectroscopy, Near-Infrared
D004883:Erucic Acids; D005961:Glucosinolates; C049811:erucic acid