title: Peroxisome proliferator-activated receptor alpha polymorphisms and postprandial lipemia in healthy men.
abstract: Peroxisome proliferator-activated receptor alpha (PPARalpha) is a ligand-dependent transcription factor that plays a key role in lipid and glucose homeostasis. This study evaluated whether variants of PPARalpha are associated with postprandial lipemia. Subjects were given a single fat load composed of 60% calories as fat, 15% as protein, and 25% as carbohydrate. Blood was drawn every hour from baseline to 6 h, then every 2.5 h to 11 h to determine triglyceride (TG) levels. The minor allele of the nonsynonymous p.Leu162Val variant was associated with higher fasting total cholesterol, LDL-cholesterol, and apolipoprotein B. There were no significant associations with all of the postprandial parameters examined. Conversely, the noncoding variant c.140+5435T>C was not associated with fasting lipid concentrations but was significantly associated with decreased postprandial TG and cholesterol in the small TG-rich lipoprotein particle. Although the minor allele carriers displayed lower mean concentrations of TG and cholesterol throughout the postprandial period, the differences were most pronounced in the latter period. These data suggest that PPARalpha variants may modulate the risk of cardiovascular disease by influencing both fasting and postprandial lipid concentrations.
authors.affiliation: Nutrition and Genomics Laboratory, Jean Mayer-U.S. Department of Agriculture Human Nutrition Research Center on Aging at Tufts University, Boston, MA, USA.
authors.forename: Toshiko
authors.initials: T
authors.lastname: Tanaka
authors.forename: Jose M
authors.initials: JM
authors.lastname: Ordovas
authors.forename: Javier
authors.initials: J
authors.lastname: Delgado-Lista
authors.forename: Francisco
authors.initials: F
authors.lastname: Perez-Jimenez
authors.forename: Carmen
authors.initials: C
authors.lastname: Marin
authors.forename: Pablo
authors.initials: P
authors.lastname: Perez-Martinez
authors.forename: Purificacion
authors.initials: P
authors.lastname: Gomez
authors.forename: Jose
authors.initials: J
authors.lastname: Lopez-Miranda
mesh_terms: D000293:Adolescent; D000328:Adult; D001055:Apolipoproteins B / Q000097:blood; D002784:Cholesterol / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood* / Q000235:genetics; D015810:Linkage Disequilibrium; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D047493:PPAR alpha / Q000235:genetics*; D011110:Polymorphism, Genetic; D019518:Postprandial Period
chemical_list: D001055:Apolipoproteins B; D008078:Cholesterol, LDL; D008055:Lipids; D047493:PPAR alpha; D002784:Cholesterol