title: [Immune response and immunopathology in inducible costimulatory molecule (ICOS) transgenic mice infected with Schistosoma japonicum].
abstract: OBJECTIVE
  To establish the ICOS transgenic mice schistosomiasis japonica model and to observe the immune response and immunopathology of the model.
  
  
  METHODS
  The transgenic mice were infected with Schistosoma japonicum. Spleen cells and sera of mice were harvested at week 4, 6, and 8 after infection. The cytokines IFN-gamma and IL-4 were measured in culture supernatans by ELISA. The serum IgG, IgGI and IgG2a were measured by ELISA at different period of infection. Liver tissue sections were prepared with HE staining. Liver granuloma formation was observed under microscope.
  
  
  RESULTS
  The expression level of IFN-y showed no significant difference between ICOS transgenic mice and control, while that of IL-4 in ICOS transgenic mice was significantly up-regulated to 20.81+/-1.95 and 25.31+/-3.37 pg/ml at week 6 and 8 respectively (P<0.01). The serum IgG and IgGl in ICOS transgenic mice were also significantly higher than those in control. Th2 differentiation index and lgC1/IgG2a were used to evaluate the immune regulation balance of Thl/Th2, and results showed that Th2 response in ICOS transgenic mice was significantly stronger than that of the control. The egg granuloma response in ICOS transgenic mice was also significantly stronger than that in control (P<0.01). The rate of egg granuloma enlargement was 24.48% and 26.37% at week 6 and 8 respectively.
  
  
  CONCLUSIONS
  The findings suggest that there is stronger Th2 type response in ICOS transgenic mice infected with Schistosoma japonicum and ICOS may play an important role in the egg granuloma formation of Schistosoma japonicum.
authors.affiliation: Department of Parasitology, Medical School, Suzhou University, Suzhou 215123, China. xia-chaoming@163.com
authors.forename: Chao-ming
authors.initials: CM
authors.lastname: Xia
authors.forename: Xiang-ke
authors.initials: XK
authors.lastname: Pu
authors.forename: Wei
authors.initials: W
authors.lastname: Gong
authors.forename: Wei
authors.initials: W
authors.lastname: Luo
authors.forename: Hui-qin
authors.initials: HQ
authors.lastname: Zhang
authors.forename: Zhong-bin
authors.initials: ZB
authors.lastname: Deng
authors.forename: Zhi-mou
authors.initials: ZM
authors.lastname: Xue
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006099:Granuloma / Q000473:pathology; D007074:Immunoglobulin G / Q000097:blood; D060889:Inducible T-Cell Co-Stimulator Protein; D007371:Interferon-gamma / Q000032:analysis; D015847:Interleukin-4 / Q000032:analysis; D008099:Liver / Q000469:parasitology / Q000473:pathology; D008109:Liver Diseases, Parasitic / Q000473:pathology; D051379:Mice; D008822:Mice, Transgenic; D012549:Schistosoma japonicum / Q000254:growth & development / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D013154:Spleen / Q000276:immunology / Q000469:parasitology / Q000473:pathology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D013997:Time Factors
chemical_list: D000909:Antibodies, Helminth; D000945:Antigens, Differentiation, T-Lymphocyte; C556694:Icos protein, mouse; D007074:Immunoglobulin G; D060889:Inducible T-Cell Co-Stimulator Protein; D015847:Interleukin-4; D007371:Interferon-gamma