title: ADAM23 plays multiple roles in neuronal differentiation of P19 embryonal carcinoma cells.
abstract: ADAM23, belonging to ADAM (A Disintegrin And Metalloprotease) protein family, is mainly expressed in brain. P19 cells could differentiate into neuroectodermal cell lineage after cell aggregates have been induced by retinoic acid (RA). In this report, we show that the post-transcriptional and post-translational processes of ADAM23 are regulated during the differentiation of P19 cells. In P19-derived neurons, ADAM23 is polarized distributed in the proximal part. To explore the possible roles of ADAM23 during P19 cell neuronal differentiation, ADAM23-RNAi P19 cell lines were established. These transfected cells could differentiate into neurofilament-expression neurons in the absence of RA, whereas wild-type P19 cell can not. These results suggest ADAM23 may play roles in both early and later stage of neuronal differentiation.
authors.affiliation: State key Laboratory of Genetics Engineering, Institute of Genetics, School of Life Science, Fudan University, 220 Han Dan Road, Shanghai, 200433, China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Sun
authors.forename: Yingming
authors.initials: Y
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Tao
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.forename: Naihe
authors.initials: N
authors.lastname: Jing
authors.forename: Chaoqun
authors.initials: C
authors.lastname: Wu
authors.forename: Kejing
authors.initials: K
authors.lastname: Deng
authors.forename: Shouyi
authors.initials: S
authors.lastname: Qiao
mesh_terms: D051722:ADAM Proteins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D054278:Embryonal Carcinoma Stem Cells; D051379:Mice; D014411:Neoplastic Stem Cells / Q000166:cytology / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D016900:Neurofilament Proteins / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D034622:RNA Interference; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins; C061195:neurofilament protein M; D014212:Tretinoin; D051722:ADAM Proteins; C121259:Adam23 protein, mouse