REMA: A computer-based mapping tool for analysis of restriction sites in multiple DNA sequences.
REMA is an interactive web-based program which predicts endonuclease cut sites in DNA sequences. It analyses multiple sequences simultaneously and predicts the number and size of fragments as well as provides restriction maps. The users can select single or paired combinations of all commercially available enzymes. Additionally, REMA permits prediction of multiple sequence terminal fragment sizes and suggests suitable restriction enzymes for maximally discriminatory results. REMA is an easy to use, web based program which will have a wide application in molecular biology research.


BACKGROUND
REMA is written in Perl and is freely available for non-commercial use. Detailed information on installation can be obtained from Jan Szubert (jan.szubert@gmail.com) and the web based application is accessible on the internet at the URL http://www.macaulay.ac.uk/rema


BACKGROUND
b.singh@macaulay.ac.uk.
Szubert
Jan
J
Faculty of Life Sciences, The University of Manchester, Oxford Road, Manchester, M13 9PT UK.
Reiff
Caroline
C
Thorburn
Andrew
A
Singh
Brajesh K
BK
D004262:DNA Restriction Enzymes / Q000737:chemistry*; D004269:DNA, Bacterial / Q000737:chemistry*; D015183:Restriction Mapping / Q000379:methods*; D012984:Software
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes