Arabidopsis MICROTUBULE-ASSOCIATED PROTEIN18 functions in directional cell growth by destabilizing cortical microtubules.
Microtubule-associated proteins (MAPs) play important roles in the regulation of microtubule function in cells. We describe Arabidopsis thaliana MAP18, which binds to microtubules and inhibits tubulin polymerization in vitro and colocalizes along cortical microtubules as patches of dot-like structures. MAP18 is expressed mostly in the expanding cells. Cells overexpressing MAP18 in Arabidopsis exhibit various growth phenotypes with loss of polarity. Cortical microtubule arrays were significantly altered in cells either overexpressing MAP18 or where it had been downregulated by RNA interference (RNAi). The cortical microtubules were more sensitive to treatment with microtubule-disrupting drugs when MAP18 was overexpressed, but more resistant when MAP18 was eliminated in cells expressing MAP18 RNAi. Our study demonstrated that MAP18 may play a role in regulating directional cell growth and cortical microtubule organization by destabilizing microtubules.
Wang
Xia
X
State Key Laboratory of Plant Physiology and Biochemistry, Department of Plant Sciences, College of Biological Sciences, China Agricultural University, Beijing 100094, China.
Zhu
Lei
L
Liu
Baoquan
B
Wang
Che
C
Jin
Lifeng
L
Zhao
Qian
Q
Yuan
Ming
M
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000166:cytology* / Q000187:drug effects / Q000235:genetics; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D016764:Cell Polarity / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D004136:Dinitrobenzenes / Q000494:pharmacology; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D008869:Microtubule-Associated Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism*; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000166:cytology / Q000254:growth & development; D030821:Plants, Genetically Modified; D011485:Protein Binding / Q000187:drug effects; D021381:Protein Transport / Q000187:drug effects; D034622:RNA Interference; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D013424:Sulfanilamides / Q000494:pharmacology; D014404:Tubulin / Q000378:metabolism
D029681:Arabidopsis Proteins; D004136:Dinitrobenzenes; C520841:MAP18 protein, Arabidopsis; D008869:Microtubule-Associated Proteins; D011994:Recombinant Proteins; D013424:Sulfanilamides; D014404:Tubulin; C012465:oryzalin