[Effect of extract of ginkgo biloba leaves on the precondition of liver graft in rat liver transplantation].
OBJECTIVE
To explore the effect of extract of ginkgo biloba leaves on the precondition of liver graft in rat liver transplantation.


METHODS
Male Sprague-Dawley rats were used as donors and recipients of orthotopic liver transplantation (OLT), and were randomly divided into extract of ginkgo biloba leaves group (Egb), NS control group (NS), and sham operation group (SO) according to whether the extract of ginkgo biloba leaves was injected by the venous (40 mg/kg) 1 h before the liver grafts harvesting. The rats were killed at 2 h, 6 h, and 24 h after the ischemia/reperfusion. The serum concentrations of ALT and AST were determined and the liver tissue were sampled to observe the expression of TNF-alpha and IL-1.


RESULTS
After the ischemia/reperfusion the serum concentration of ALT and AST and expressions of TNF-alpha and IL-1 in the hepatic tissue in the NS group significantly increased (p<0.01), and the hepatocytic morphologic change was obvious compared with the SO group. The treatment of ginkgo biloba extract significantly decreased the serum concentration of ALT and AST and the expressions of TNF-alpha and IL-1 in the hepatic tissue in EGb group compared with the NS group (p<0.01), and relieved the hepatocyte swelling and necrosis.


CONCLUSIONS
Ginkgo bilobA extract may decrease the release of TNF-alpha and IL-1 by inhibiting activation of kuffer cells and regulate the cell factors to protect the live.
Zhou
Jie-Bin
JB
Institute of Organ Transplantation, Third Xiangya Hospital, Central South University, Department of General Surgery, Changsha Central Hospital, Changsha 410013, China. zhoujiebin2003@163.com
Yang
Xue-Kang
XK
Ye
Qi-Fa
QF
Ming
Ying-Zi
YZ
Xia
Zong-Jiang
ZJ
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D020441:Ginkgo biloba / Q000737:chemistry*; D007375:Interleukin-1 / Q000096:biosynthesis; D008099:Liver / Q000098:blood supply / Q000187:drug effects / Q000378:metabolism; D016031:Liver Transplantation; D008297:Male; D018515:Plant Leaves / Q000737:chemistry*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000097:blood / Q000378:metabolism / Q000517:prevention & control*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D004365:Drugs, Chinese Herbal; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase