In vitro fermentation characteristics of selected glucose-based polymers by canine and human fecal bacteria.
This research evaluated fermentation characteristics (short-chain fatty acid [SCFA] production, pH, and gas production) resulting from fermentation of glucose-based carbohydrates using canine (n = 3) and human (n = 3) fecal inoculum. Substrates included lyophilized canine ileal digesta containing maltodextrin, gamma-cyclodextrin, high molecular weight (MW) pullulan (MW 100000), or low MW pullulan (MW 6300) obtained from an in vivo experiment. Fermentation for 6 and 10 h with human fecal microflora resulted in higher gas and SCFA production than did canine fecal microflora. High MW pullulan fermentation resulted in the highest (p < 0.05) gas production and lowest (p < 0.05) pH for both dogs and humans. Total SCFA production was highest (p < 0.05) for low MW pullulan fermented by canine microflora, and for gamma-cyclodextrin, high MW pullulan, and low MW pullulan fermented by human microflora. Differences were noted in fermentation characteristics of substrates present in ileal digesta.
Spears
Julie K
JK
Department of Animal Sciences, University of Illinois, Urbana, IL 61801, USA. gcfahey@uiuc.edu
Karr-Lilienthal
Lisa K
LK
Bauer
Laura L
LL
Murphy
Michael R
MR
Fahey
George C
GC
D000818:Animals; D002482:Cellulose / Q000378:metabolism; D003505:Cyclodextrins / Q000378:metabolism; D004285:Dogs; D005232:Fatty Acids, Volatile / Q000032:analysis*; D005243:Feces / Q000382:microbiology*; D005285:Fermentation; D005936:Glucans / Q000737:chemistry / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007082:Ileum / Q000382:microbiology; D008970:Molecular Weight; D011134:Polysaccharides / Q000378:metabolism
D003505:Cyclodextrins; D005232:Fatty Acids, Volatile; D005936:Glucans; D011134:Polysaccharides; C041953:cyclodextrin polymer; C008315:maltodextrin; C009109:pullulan; D002482:Cellulose; D005947:Glucose