title: Clearance of dying autophagic cells of different origin by professional and non-professional phagocytes.
abstract: MCF-7 cells undergo autophagic death upon tamoxifen treatment. Plated on non-adhesive substratum these cells died by anoikis while inducing autophagy as revealed by monodansylcadaverine staining, elevated light-chain-3 expression and electron microscopy. Both de novo and anoikis-derived autophagic dying cells were engulfed by human macrophages and MCF-7 cells. Inhibition of autophagy by 3-methyladenine abolished engulfment of cells dying through de novo autophagy, but not those dying through anoikis. Blocking exposure of phosphatidylserine (PS) on both dying cell types inhibited phagocytosis by MCF-7 but not by macrophages. Gene expression profiling showed that though both types of phagocytes expressed full repertoire of the PS recognition and signaling pathway, macrophages could evolve during engulfment of de novo autophagic cells the potential of calreticulin-mediated processes as well. Our data suggest that cells dying through autophagy and those committing anoikis with autophagy may engage in overlapping but distinct sets of clearance mechanisms in professional and non-professional phagocytes.
authors.affiliation: Department of Biochemistry, Apoptosis and Genomics Research Group of the Hungarian Academy of Sciences, Biophysics and Cell Biology, Research Center for Molecular Medicine, University of Debrecen, Debrecen, Hungary.
authors.forename: G
authors.initials: G
authors.lastname: Petrovski
authors.forename: G
authors.initials: G
authors.lastname: Zahuczky
authors.forename: K
authors.initials: K
authors.lastname: Katona
authors.forename: G
authors.initials: G
authors.lastname: Vereb
authors.forename: W
authors.initials: W
authors.lastname: Martinet
authors.forename: Z
authors.initials: Z
authors.lastname: Nemes
authors.forename: W
authors.initials: W
authors.lastname: Bursch
authors.forename: L
authors.initials: L
authors.lastname: Fésüs
mesh_terms: D023102:Anoikis / Q000187:drug effects / Q000502:physiology; D001343:Autophagy / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D016923:Cell Death / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D045744:Cell Line, Tumor; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D015151:Immunoblotting; D008264:Macrophages / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D010586:Phagocytes / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D010587:Phagocytosis / Q000187:drug effects; D010718:Phosphatidylserines / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013629:Tamoxifen / Q000494:pharmacology; D014158:Transcription, Genetic
chemical_list: D010718:Phosphatidylserines; D013629:Tamoxifen