title: Method to enhance transfection efficiency of cell lines and placental fibroblasts.
abstract: We report a method that allows for a 2-9-fold increase in transfection efficiency compared to the standard cationic lipid-based protocol. The method involves a brief incubation of freshly trypsinized cells with transfection complexes, followed by incubation in cell growth medium containing serum and antibiotics. The method is simple, cost-effective, and can be applied to both DNA and siRNA transfections as well as to a variety of cell types including hard-to-transfect human placental fibroblasts.
authors.affiliation: Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, 300 George Street, Suite 770, Room 759F, New Haven, CT 06511, USA.
authors.forename: M
authors.initials: M
authors.lastname: Zhang
authors.forename: S
authors.initials: S
authors.lastname: Guller
authors.forename: Y
authors.initials: Y
authors.lastname: Huang
mesh_terms: D002412:Cations; D002460:Cell Line; D004247:DNA; D005347:Fibroblasts; D006801:Humans; D014162:Transfection
chemical_list: D002412:Cations; D004247:DNA