title: Evaluation of antifungal and DNA-damaging activities of alkaloids from branches of Porcelia macrocarpa.
abstract: Bioactivity-guided fractionation of the alkaloidal fractions of the CH2Cl2 extract from branches of Porcelia macrocarpa using mutant yeast strains of Saccharomyces cerevisiae and fungi Cladosporium cladosporioides and C. sphaerospermum led to the isolation of bioactive alkaloids: two tetrahydrobenzylisoquinolines (1 and 2), two aporphines (3 and 4), one proaporphine (5), one oxoaporphine (6), four azantraquinones (7-10) and four azafluorenones (11-14). The alkaloids cleistopholine (7) and 6-methoxycleistopholine (8) showed the highest fungitoxic activity while the mixture of 6- and 7-methoxyonychine (12+13) and 6,7-dimethoxyonychine (14) showed a weak DNA-damaging potential.
authors.affiliation: Centro de Ciências e Humanidades, Universidade Presbiteriana Mackenzie, São Paulo, SP, Brazil.
authors.forename: João Henrique G
authors.initials: JH
authors.lastname: Lago
authors.forename: Mariana H
authors.initials: MH
authors.lastname: Chaves
authors.forename: Mariane Cruz C
authors.initials: MC
authors.lastname: Ayres
authors.forename: Débora G
authors.initials: DG
authors.lastname: Agripino
authors.forename: Maria Cláudia M
authors.initials: MC
authors.lastname: Young
mesh_terms: D000470:Alkaloids / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D029061:Annonaceae; D000935:Antifungal Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D002963:Cladosporium / Q000187:drug effects; D004249:DNA Damage; D006801:Humans; D008826:Microbial Sensitivity Tests; D008517:Phytotherapy; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D012441:Saccharomyces cerevisiae / Q000187:drug effects
chemical_list: D000470:Alkaloids; D000935:Antifungal Agents; D010936:Plant Extracts