Expression analysis of multiple dnaK genes in the cyanobacterium Synechococcus elongatus PCC 7942.
We analyzed the stress responses of three dnaK homologues (dnaK1, dnaK2, and dnaK3) in the cyanobacterium Synechococcus elongatus PCC 7942. A reporter assay showed that under stress conditions the expression of only the dnaK2 gene was induced, suggesting a functional assignment of these homologues. RNA blot hybridization indicated a typical stress response of dnaK2 to heat and high-light stress. Primer extension mapping showed that dnaK2 was transcribed from similar sites under various stress conditions. Although no known sequence motif was detected in the upstream region, a 20-bp sequence element was highly conserved in dnaK2; it was essential not only for the stress induction but also for the basal expression of dnaK2. The ubiquitous upstream localization of this element in each heat shock gene suggests its important role in the cyanobacterial stress response.
Sato
Masumi
M
Department of Bioscience, Tokyo University of Agriculture, Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
Nimura-Matsune
Kaori
K
Watanabe
Satoru
S
Chibazakura
Taku
T
Yoshikawa
Hirofumi
H
D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D017124:Conserved Sequence; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D018869:Heat-Shock Response / Q000235:genetics*; D008027:Light; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D012329:RNA, Bacterial / Q000235:genetics; D046940:Synechococcus / Q000235:genetics*
D001426:Bacterial Proteins; C102626:DnaK3 protein, Synechococcus; D018840:HSP70 Heat-Shock Proteins; D008565:Membrane Proteins; D012329:RNA, Bacterial