title: Counter-current chromatography separation scaled up from an analytical column to a production column.
abstract: An analytical separation was performed on an analytical J-type counter-current chromatography (CCC) instrument using a 5.4 ml column, with a 1 ml/min mobile phase flow rate. This separation had a resolution of 0.69 and was achieved in 10 min. The same separation was performed using two 2300 ml columns connected in series at a flow rate of 850 ml/min using a production scale J-type centrifuge. This production scale separation was also obtained in 10 min with a resolution of 0.71. This represents an 850 times increase in productivity. This paper presents these separations and the underlying scale up theory.
authors.affiliation: Brunel Institute for Bioengineering, Brunel University, Uxbridge UB8 3PH, UK. philip.wood@brunel.ac.uk
authors.forename: Philip
authors.initials: P
authors.lastname: Wood
authors.forename: Svetlana
authors.initials: S
authors.lastname: Ignatova
authors.forename: Lee
authors.initials: L
authors.lastname: Janaway
authors.forename: David
authors.initials: D
authors.lastname: Keay
authors.forename: David
authors.initials: D
authors.lastname: Hawes
authors.forename: Ian
authors.initials: I
authors.lastname: Garrard
authors.forename: Ian A
authors.initials: IA
authors.lastname: Sutherland
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000295:instrumentation* / Q000379:methods* / Q000639:trends; D015203:Reproducibility of Results