[Cloning and sequence analysis of a partial gene of Trichomonas vaginalis dsRNA virus].
A pair of degenerate primers were designed following the published nucleotide sequence of Trichomonas vaginalis virus(U08999, NC003873, NC003824, NC003834). Using the total nucleic acids extracted from the Trichomonas vaginalis as template, RT-PCR was performed with the primers to obtain a fragment of the TVV. The product was cloned, sequenced and compared with the sequences available in the GenBank. The size of the amplified gene was 1 454bp, which shares 82.9% sequence identity with the Trichomonas vaginalis virus T1.
Zhao
Yue-ping
YP
College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China.
Zhang
Xi-chen
XC
Chen
Li-feng
LF
Li
Jian-hua
JH
Yin
Ji-gang
JG
Liu
Quan
Q
Gong
Peng-tao
PT
D000818:Animals; D003001:Cloning, Molecular; D005814:Genes, Viral; D012328:RNA Viruses / Q000235:genetics*; D012330:RNA, Double-Stranded; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014246:Trichomonas vaginalis / Q000821:virology*
D012330:RNA, Double-Stranded