Adherence of enterohemorrhagic Escherichia coli O157, O26, and O111 strains to bovine intestinal explants ex vivo.
We used bovine intestinal organ culture to study infection by enterohemorrhagic Escherichia coli serogroups O157, O26, and O111. We show colonization and attaching and effacing lesion formation on explants derived from the ileum, colon, and rectum. Intimin and Tir were detected at the sites of adherent bacteria; Tir was essential for colonization.
Girard
Francis
F
Division of Cell and Molecular Biology, Imperial College London, London, UK.
Dziva
Francis
F
van Diemen
Pauline
P
Phillips
Alan D
AD
Stevens
Mark P
MP
Frankel
Gad
G
D018829:Adhesins, Bacterial / Q000378:metabolism; D000818:Animals; D001422:Bacterial Adhesion; D002417:Cattle; D054324:Enterohemorrhagic Escherichia coli / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000378:metabolism; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000382:microbiology*; D011956:Receptors, Cell Surface / Q000378:metabolism; D013045:Species Specificity; D046509:Tissue Culture Techniques
D018829:Adhesins, Bacterial; D029968:Escherichia coli Proteins; D011956:Receptors, Cell Surface; C109287:Tir protein, E coli; C073819:eaeA protein, E coli