[Prolactin modulation of theophylline inhibitory action on maturation of bovine oocyte-cumulus complexes in vitro].
The comparative investigation of the individual and joint impact of prolactin (PRL, 50 ng/ml) and theophylline (TP), a nonselective inhibitor of phosphodiesterases, on nuclear maturation of bovine oocytes and the expansion of cumulus cells enclosing the oocytes was carried out using a model of in vitro culturing. It has been shown that TP (5 mM) exerts a short-term inhibitory action on oocyte meiosis reinitiation and blocks it at diakinesis and metaphase I stages as well as inhibits the cumulus expansion. The addition of PRL to the medium containing TP caused the decrease in the rate of oocytes at diplotene stage after 6 h of culturing and the increase in the rate of oocytes attained the closing stages of maturation after 24 h of culturing. Furthermore, PRL suppressed partly the inhibitory impact of TP on the expansion of cumulus cells. The data obtained suggest the signal cascade induced by PRL in bovine oocyte-cumulus complexes to be compled with cAMP-dependent intracellular pathway.
Lebedeva
I Iu
IIu
Skotti
O S
OS
Kuz'mina
T I
TI
D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D005260:Female; D008540:Meiosis / Q000187:drug effects; D009865:Oocytes / Q000254:growth & development* / Q000378:metabolism; D010726:Phosphodiesterase Inhibitors / Q000378:metabolism* / Q000494:pharmacology; D011388:Prolactin / Q000494:pharmacology / Q000502:physiology*; D015290:Second Messenger Systems / Q000187:drug effects / Q000502:physiology; D013806:Theophylline / Q000378:metabolism* / Q000494:pharmacology
D003470:Culture Media; D010726:Phosphodiesterase Inhibitors; D011388:Prolactin; D013806:Theophylline