Amphiphilic polyether branched molecules to increase the circulation time of cationic particles.
The preparation, physicochemical and biological properties of amphiphilic polyether branched molecules is described. These 'bunch shaped' molecules when inserted into cationic liposomes/DNA complexes have shown efficient surface charge shielding. As a consequence they efficiently inhibited the non specific interactions with blood components and significantly enhanced circulation time of the particles in the blood track. Formulations containing these molecules compared positively with those containing PEG lipids, providing a 5-fold increase in circulation time.
Garinot
Marie
M
Inserm, U640, Paris F-75006, France.
Mignet
Nathalie
N
Largeau
Celine
C
Seguin
Johanne
J
Scherman
Daniel
D
Bessodes
Michel
M
D000818:Animals; D001776:Blood Circulation Time; D002412:Cations / Q000737:chemistry; D045744:Cell Line, Tumor; D004247:DNA / Q000737:chemistry* / Q000493:pharmacokinetics; D004355:Drug Stability; D004987:Ethers / Q000138:chemical synthesis / Q000737:chemistry* / Q000493:pharmacokinetics; D005260:Female; D008081:Liposomes; D051379:Mice; D008810:Mice, Inbred C57BL; D015394:Molecular Structure; D010316:Particle Size; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry* / Q000493:pharmacokinetics; D013499:Surface Properties; D013997:Time Factors; D014162:Transfection
D002412:Cations; D004987:Ethers; D008081:Liposomes; D011108:Polymers; D004247:DNA