Random access three-dimensional two-photon microscopy.
We propose a two-photon microscope scheme capable of real-time, three-dimensional investigation of the electric activity pattern of neural networks or signal summation rules of individual neurons in a 0.6 mm x 0.6 mm x 0.2 mm volume of the sample. The points of measurement are chosen according to a conventional scanning two-photon image, and they are addressed by separately adjustable optical fibers. This allows scanning at kilohertz repetition rates of as many as 100 data points. Submicrometer spatial resolution is maintained during the measurement similarly to conventional two-photon microscopy.
Rózsa
Balázs
B
Department of Pharmacology, Institute of Experimental Medicine, Hungary.
Katona
Gergely
G
Vizi
E Sylvester
ES
Várallyay
Zoltán
Z
Sághy
Attila
A
Valenta
Lásló
L
Maák
Pál
P
Fekete
Júlia
J
Bányász
Akos
A
Szipocs
Róbert
R
D000200:Action Potentials / Q000502:physiology*; D002478:Cells, Cultured; D005336:Fiber Optic Technology / Q000295:instrumentation*; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000295:instrumentation* / Q000379:methods; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation / Q000379:methods*; D009415:Nerve Net / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D055100:Optical Fibers