title: Fine mapping of the Pc locus of Sorghum bicolor, a gene controlling the reaction to a fungal pathogen and its host-selective toxin.
abstract: Milo disease in sorghum is caused by isolates of the soil-borne fungus Periconia circinata that produce PC-toxin. Susceptibility to milo disease is conditioned by a single, semi-dominant gene, termed Pc. The susceptible allele (Pc) converts to a resistant form (pc) spontaneously at a gametic frequency of 10(-3) to 10(-4). A high-density genetic map was constructed around the Pc locus using DNA markers, allowing the Pc gene to be delimited to a 0.9 cM region on the short arm of sorghum chromosome 9. Physically, the Pc-region was covered by a single BAC clone. Sequence analysis of this BAC revealed twelve gene candidates. Several of the predicted genes in the region are homologous to disease resistance loci, including one NBS-LRR resistance gene analogue that is present in multiple tandem copies. Analysis of pc isolines derived from Pc/Pc sorghum suggests that one or more members of this NBS-LRR gene family are the Pc genes that condition susceptibility.
authors.affiliation: Department of Genetics, University of Georgia, Athens, GA, 30602, USA.
authors.forename: Ervin D
authors.initials: ED
authors.lastname: Nagy
authors.forename: Tso-Ching
authors.initials: TC
authors.lastname: Lee
authors.forename: Wusirika
authors.initials: W
authors.lastname: Ramakrishna
authors.forename: Zijun
authors.initials: Z
authors.lastname: Xu
authors.forename: Patricia E
authors.initials: PE
authors.lastname: Klein
authors.forename: Phillip
authors.initials: P
authors.lastname: SanMiguel
authors.forename: Chiu-Ping
authors.initials: CP
authors.lastname: Cheng
authors.forename: Jingling
authors.initials: J
authors.lastname: Li
authors.forename: Katrien M
authors.initials: KM
authors.lastname: Devos
authors.forename: Keith
authors.initials: K
authors.lastname: Schertz
authors.forename: Larry
authors.initials: L
authors.lastname: Dunkle
authors.forename: Jeffrey L
authors.initials: JL
authors.lastname: Bennetzen
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001203:Ascomycota / Q000472:pathogenicity*; D022202:Chromosomes, Artificial, Bacterial; D032461:Chromosomes, Plant; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D018744:DNA, Plant; D018628:Gene Dosage; D020440:Gene Duplication; D005799:Genes, Dominant; D017343:Genes, Plant; D005819:Genetic Markers; D005838:Genotype; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D018807:Polymorphism, Single-Stranded Conformational; D011995:Recombination, Genetic; D017386:Sequence Homology, Amino Acid; D045868:Sorghum / Q000235:genetics*; D014118:Toxins, Biological / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers; D014118:Toxins, Biological