Identification of quantum dot bioconjugates and cellular protein co-localization by hybrid gel blotting.
New approaches are needed to address the interaction of nanoparticles and cellular proteins at the molecular level. We present a modification of PAGE co-immunoprecipitation, QD-based PA-AGE electrophoresis blotting, and apply this to identify quantum dot (QD) bioconjugate-cellular protein association. This method provides the capability to isolate and evaluate the action of QD bioconjugate-protein complexes in intact cells and to correlate these identified interactions with their location in cells.
Liu
Hong Yan
HY
Department of Biomedical Engineering, Oregon Health & Science University, 3303 SW Bond Avenue, 13B, Portland, Oregon 97239, USA.
Vu
Tania Q
TQ
D002468:Cell Physiological Phenomena; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D007118:Immunoassay / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D025941:Protein Interaction Mapping / Q000379:methods*; D045663:Quantum Dots; D018511:Systems Integration