title: New immortalized cell lines of patients with small supernumerary marker chromosome: towards the establishment of a cell bank.
abstract: Sixteen newly established cell lines with small supernumerary marker chromosomes (sSMC) derived from chromosomes 1, 2, 4, 6, 7, 8, 14, 15, 16, 18, 19, 21, and 22 are reported. Two sSMC are neocentric and derived from 15q24.1-qter and 2q35-q36, respectively. Two further cases each present with two sSMC of different chromosomal origin. sSMC were characterized by multicolor fluorescence in situ hybridization for their chromosomal origin and genetic content. Moreover, uniparental disomy of the sister chromosomes of the sSMC was excluded in all nine cases studied for that reason. The 16 cases provide information to establish a refined genotype-phenotype correlation of sSMC and are available for future studies.
authors.affiliation: Institute of Human Genetics, Charité Campus Virchow, Berlin, Germany. i8lith@mti.uni-jena.de
authors.forename: Holger
authors.initials: H
authors.lastname: Tönnies
authors.forename: Joanna
authors.initials: J
authors.lastname: Pietrzak
authors.forename: Ewa
authors.initials: E
authors.lastname: Bocian
authors.forename: Kay
authors.initials: K
authors.lastname: MacDermont
authors.forename: Alma
authors.initials: A
authors.lastname: Kuechler
authors.forename: Britta
authors.initials: B
authors.lastname: Belitz
authors.forename: Udo
authors.initials: U
authors.lastname: Trautmann
authors.forename: Angela
authors.initials: A
authors.lastname: Schmidt
authors.forename: Berndt
authors.initials: B
authors.lastname: Schulze
authors.forename: Laura
authors.initials: L
authors.lastname: Rodríguez
authors.forename: Franz
authors.initials: F
authors.lastname: Binkert
authors.forename: Catharine
authors.initials: C
authors.lastname: Yardin
authors.forename: Nadezda
authors.initials: N
authors.lastname: Kosyakova
authors.forename: Marianne
authors.initials: M
authors.lastname: Volleth
authors.forename: Hasmik
authors.initials: H
authors.lastname: Mkrtchyan
authors.forename: Isolde
authors.initials: I
authors.lastname: Schreyer
authors.forename: Ferdinand
authors.initials: F
authors.lastname: von Eggeling
authors.forename: Anja
authors.initials: A
authors.lastname: Weise
authors.forename: Kristin
authors.initials: K
authors.lastname: Mrasek
authors.forename: Thomas
authors.initials: T
authors.lastname: Liehr
mesh_terms: D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism / Q000821:virology; D018070:Biological Specimen Banks; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D002869:Chromosome Aberrations; D020223:Chromosome Painting / Q000379:methods*; D005260:Female; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D007621:Karyotyping; D008297:Male; D008957:Models, Genetic; D024182:Uniparental Disomy