Cell adhesion and growth to Peptide-patterned supported lipid membranes.
Lipid vesicles displaying RGD peptide amphiphiles were fused with glass coverslips to control the ability of these surfaces to support cell adhesion and growth. Cell adhesion was prevented on phosphatidylcholine bilayers in the absence of RGD, whereas cells adhered and grew in the presence of accessible RGD amphiphiles. This specific interaction between cells and RGD peptides was further explored in a concentration-dependent fashion by creating surface composition arrays using microfluidics. For the range of concentrations studied adhesion and growth were favored by increased peptide concentration, but this concentration dependence was found to diminish in the higher concentration regions of the array. Developing peptide composition gradients in a membrane environment is demonstrated as an effective method to screen biological probes for cell adhesion and growth.
Stroumpoulis
Dimitrios
D
Department of Chemical Engineering and Materials Research Laboratory, University of California, Santa Barbara, California 93106, USA.
Zhang
Haining
H
Rubalcava
Leticia
L
Gliem
Jill
J
Tirrell
Matthew
M
D000818:Animals; D002448:Cell Adhesion; D005347:Fibroblasts / Q000166:cytology*; D008051:Lipid Bilayers; D051379:Mice; D044085:Microfluidics; D009842:Oligopeptides
D008051:Lipid Bilayers; D009842:Oligopeptides; C421251:RGD891