title: Mud-P22.
abstract: Mud-P22 derivatives are hybrids between phage Mu and P22 that can be inserted at essentially any desired site on the Salmonella chromosome (Benson and Goldman, 1992; Youderian et al., 1988). Induction of Mud-P22 insertions yields phage particles that, as a population, carry chromosomal DNA from the region between 150 and 250Kb on one side of the insertion. Thus, phage lysates from a representative set of Mud-P22 insertions into the S. typhimurium chromosome yield an ordered library of DNA that provides powerful tools for the genetic and physical analysis of the Salmonella genome. Although Mud-P22 has not yet been used in other species, this approach should be applicable in a variety of other bacteria as well.
authors.affiliation: Department of Biology, Center for Microbial Sciences, San Diego State University, San Diego, CA, USA.
authors.forename: Jenny A
authors.initials: JA
authors.lastname: Crain
authors.forename: Stanley R
authors.initials: SR
authors.lastname: Maloy
mesh_terms: D017100:Bacteriophage P22 / Q000235:genetics; D010583:Bacteriophage mu / Q000235:genetics; D002876:Chromosomes, Bacterial / Q000235:genetics; D015723:Gene Library; D016680:Genome, Bacterial; D016254:Mutagenesis, Insertional; D012483:Salmonella Phages / Q000235:genetics*; D012486:Salmonella typhimurium / Q000235:genetics*