[The clinical significance of molecular findings in surgically resected margins of the primary tumor laryngeal carcinoma].
OBJECTIVE
To study the relationship at molecular level between the resection range of laryngeal squamous cell carcinoma (LSCC) and prognosis.


METHODS
Immunohistochemistry method was used to detected the expression of EGFR, p27,Bcl-2 and p53 in surgically resected margins of the 23 patients with LSCC. The relationship between the genes and tumour recurrence were observed by the follow-up.


RESULTS
Seven margin tissues of tumors had detectable Bcl-2 expression; eleven margin tissues overexpressed p27; and three expressed p53 and EGFR. Four of the 23 patients have had tumor recurrence and their expression of p27, p53 and EGFR were negative; the expression of Bcl-2 was positive in two patients of the four recurrences patients. The rest were alive unrelated causes without clinical (good outcome). Expression of neither EGFR nor p53 was associated with recurrence. Only p27 was significantly associated with outcome and disease-free survival.


CONCLUSIONS
These data indicate that assessing expression of multiple genes with immunohistochemistry in margin tissues is likely to be useful for surgically treated advanced laryngeal carcinoma. The expression of the p27 is more important.
Jin
Xiaojie
X
Department of Otolaryngology-Head and Neck Surgery, Renji Hospital, Shanghai Jiaotong University, Shanghai, 200001, China.
Qian
Minfei
M
Xu
Yanping
Y
Zheng
Luying
L
Wang
Jiadong
J
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000378:metabolism; D066246:ErbB Receptors / Q000378:metabolism; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D050760:Cyclin-Dependent Kinase Inhibitor p27; C512478:EGFR protein, human; D066246:ErbB Receptors