Enantioseparation and determination of 2,2-dimethylcyclopropanecarboxamide and corresponding acid in the bioconversion broth by gas chromatography.
A simple and sensitive method employing gas chromatography was developed for the enantioseparation and determination of 2,2-dimethylcyclopropanecarboxamide and its acid in the bioconversion broth. Samples were recovered in high yield by extracting the broth with equal volumes of ethyl acetate twice. Separation of the four enantiomers was performed on a gamma-cyclodextrin based chiral column BGB-175. The effect of column flow rate and temperature on the retention and resolution of the enantiomers was investigated. The proposed method exhibited good linearity, repeatability and precision, and was successfully used to monitor and control the bioconversion process of 2,2-dimethylcyclopropanecarboxamide.
Zheng
Ren-Chao
RC
Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310032, People's Republic of China.
Zheng
Yu-Guo
YG
Shen
Yin-Chu
YC
D000327:Adsorption; D000577:Amides / Q000737:chemistry* / Q000302:isolation & purification*; D002384:Catalysis; D002849:Chromatography, Gas / Q000379:methods*; D003521:Cyclopropanes / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D042742:Delftia / Q000378:metabolism; D007700:Kinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013237:Stereoisomerism; D013678:Technology, Pharmaceutical / Q000379:methods; D013696:Temperature; D047408:gamma-Cyclodextrins / Q000737:chemistry
D000577:Amides; D003521:Cyclopropanes; D047408:gamma-Cyclodextrins; C015766:cyclopropanecarboxylic acid; C023792:gamma-cyclodextrin