Multifunctional encoded particles for high-throughput biomolecule analysis.
High-throughput screening for genetic analysis, combinatorial chemistry, and clinical diagnostics benefits from multiplexing, which allows for the simultaneous assay of several analytes but necessitates an encoding scheme for molecular identification. Current approaches for multiplexed analysis involve complicated or expensive processes for encoding, functionalizing, or decoding active substrates (particles or surfaces) and often yield a very limited number of analyte-specific codes. We present a method based on continuous-flow lithography that combines particle synthesis and encoding and probe incorporation into a single process to generate multifunctional particles bearing over a million unique codes. By using such particles, we demonstrate a multiplexed, single-fluorescence detection of DNA oligomers with encoded particle libraries that can be scanned rapidly in a flow-through microfluidic channel. Furthermore, we demonstrate with high specificity the same multiplexed detection using individual multiprobe particles.
Pregibon
Daniel C
DC
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Toner
Mehmet
M
Doyle
Patrick S
PS
D002623:Chemistry Techniques, Analytical / Q000379:methods*; D004247:DNA / Q000032:analysis*; D005453:Fluorescence; D005456:Fluorescent Dyes; D046210:Microfluidic Analytical Techniques; D044085:Microfluidics; D015336:Molecular Probe Techniques / Q000191:economics; D010316:Particle Size; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D005456:Fluorescent Dyes; D004247:DNA