title: [Studies on diterpenes in needles of Pinus sylvestris].
abstract: OBJECTIVE
  To research the constituents in needles of Pinus sylvestris.
  
  
  METHODS
  Repeated column chromatography and preparation HPLC are used for compound isolation, and their structures were elucidated on the basis of spectral data analysis.
  
  
  RESULTS
  Six compounds, pinifolic acid (1), 15-oxo-8 (17) -labden-18-oic acid (2) , 15-acetoxy-labd-8 ( 17)-en-18-oic acid (3), dehydroabietic acid (4), 7alpha-hydroxydehydroabietic acid (5), 7beta-hydroxydehydroabietic acid (6) were isolated from the needles of P. sylvestris.
  
  
  CONCLUSIONS
  Compound 3-6 were isolated from the needles of P. sylvestris for the first time, and compound 3 is a new natural product. The petroleum ether and EtOAc extracts showed significant cytotoxic effects to Hela and A549. Compounds 2, 4-6 revealed a positive distinction compared to the control group.
authors.affiliation: The School of Pharmacy, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Zhang
authors.forename: Hong-quan
authors.initials: HQ
authors.lastname: Duan
authors.forename: Zhi
authors.initials: Z
authors.lastname: Yao
authors.forename: Qin
authors.initials: Q
authors.lastname: Pan
authors.forename: Fu-geng
authors.initials: FG
authors.lastname: Zhang
mesh_terms: D045784:Abietanes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D015394:Molecular Structure; D041605:Pinus sylvestris / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D013329:Structure-Activity Relationship
chemical_list: C528007:15-acetoxy-labd-8(17)-en-18-oic acid; C528300:7alpha-hydroxydehydroabietic acid; D045784:Abietanes; D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; C013913:dehydroabietic acid