Studies on endotoxin removal mechanism of adsorbents with amino acid ligands.
In this paper, a series of adsorbents with different amino acid ligands for endotoxin removal were prepared and endotoxin adsorption capacities (EAC) in aqueous solution were studied using an affinity column. The results showed that the property and structure of amino acid ligands have great influence on EAC. As the increasing of isoelectric point and polarity of amino acids ligands, EACs of the adsorbents increased. In addition, computer simulation method was employed to a further investigation on the interaction between endotoxins and ligands. Based on the results, some adsorbents were applied to remove endotoxin from endotoxemia rabbit's serum. Similar adsorption results were observed and the removal efficiency of adsorbents with Arg, Ser ligands is up to 78%.
Wei
Zhong
Z
Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, Nankai University, Tianjin 300071, China.
Huang
Wei
W
Li
Jihong
J
Hou
Guanghui
G
Fang
Jie
J
Yuan
Zhi
Z
D000327:Adsorption; D000596:Amino Acids / Q000737:chemistry*; D003198:Computer Simulation; D004731:Endotoxins / Q000737:chemistry / Q000302:isolation & purification*; D008024:Ligands; D008958:Models, Molecular; D012996:Solutions
D000596:Amino Acids; D004731:Endotoxins; D008024:Ligands; D012996:Solutions