Verification and rapid identification of soybean rhizobia in Indian soils.
Sixty root nodule isolates of soybean rhizobia indigenous to eight field sites in India were characterized using PCR-RFLP for repeated sequence RSalpha a 1195-bp DNA fragment, indole acetic acid production, and nitrogenase activity. Site-dependent variations were observed in terms of IAA production and nitrogenase activities. RSalpha was conserved in slow-growing soybean rhizobia across locations and sites and was absent in other Rhizobiaceae members and other bacterial genera. The results suggest that RSalpha can be a useful molecular marker for slow-growing soybean rhizobia. The study also showed the low presence of soybean nodulating fast growers in Indian soils.
Annapurna
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K
Division of Microbiology, Indian Agricultural Research Institute, New Delhi, 110 012, India. annapurna96@yahoo.co.in
Balakrishnan
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Vital
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D000114:Acetylene / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D007194:India; D007210:Indoleacetic Acids / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid; D012231:Rhizobium / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D012988:Soil Microbiology; D013025:Glycine max / Q000382:microbiology*
D004269:DNA, Bacterial; D007210:Indoleacetic Acids; D000114:Acetylene