Administration of anti-type II collagen antibody sustains footpad swelling of mice caused by a delayed-type hypersensitivity reaction and induces severe arthritis.
Delayed-type hypersensitivity (DTH) is an immune reaction induced by antigen. In the mice footpads at which DTH is elicited, transient swellings which usually peaks at 24-48 h after the antigen challenge are observed. We found that the footpad swellings of mice are sustained for at least 7 days after the antigen challenge if the mice were injected with anti-type II collagen monoclonal antibody (anti-CII MoAb) before the antigen challenge. A histological section of the swelled hindpaw revealed that severe joint inflammation and bone destruction was induced. These features were not observed in the footpads of the DTH-induced mice. Analysis of the inflammatory reaction induced by both the DTH and the anti-CII MoAb injection, here named as DTH arthritis, revealed the following: (1) DTH arthritis is elicited in an antigen-specific manner; and (2) the development of DTH arthritis is mediated by antigen-specific T cells, especially CD4+ T cells.
Tanaka
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Biological Research Laboratories, Sankyo Co., Ltd, Tokyo, Japan.
Kagari
T
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Doi
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Shimozato
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T
D019264:Adoptive Transfer; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001169:Arthritis, Experimental / Q000276:immunology* / Q000473:pathology; D001842:Bone and Bones / Q000473:pathology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D024043:Collagen Type II / Q000276:immunology*; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D006968:Hypersensitivity, Delayed / Q000276:immunology* / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D013583:Synovial Membrane / Q000473:pathology
D000911:Antibodies, Monoclonal; D024043:Collagen Type II; D018984:Epitopes, T-Lymphocyte