title: Low-frequency electrophoretic actuation of nanoscale optoentropic transduction mechanisms.
abstract: Inherent bistabilities within DNA-assembled fluorescent resonant energy transfer systems demonstrated time-varying optical signals in response to an electrophoretic driving force. Frequency responses of electrophoretically driven FRET systems were shown to be sequence specific. Integration of these signals over time gave near single-molecule sensitivity within a high background of autofluorescence. This research suggests that externally driven nanoscale mechanical systems may help improve information flow within morphologically intact specimens.
authors.affiliation: Department of Bioengineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA.
authors.forename: Benjamin David
authors.initials: BD
authors.lastname: Sullivan
authors.forename: Dietrich A
authors.initials: DA
authors.lastname: Dehlinger
authors.forename: Sanja
authors.initials: S
authors.lastname: Zlatanovic
authors.forename: Sadik A
authors.initials: SA
authors.lastname: Esener
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Heller
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D004586:Electrophoresis / Q000379:methods*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D017403:In Situ Hybridization / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D004247:DNA