title: Fully packed capillary electrochromatographic microchip with self-assembly colloidal silica beads.
abstract: A fully packed capillary electrochromatographic (CEC) microchip showing improved solution and chip handling was developed. Microchannels for the CEC microchip were patterned on a cyclic olefin copolymer substrate by injection molding and packed fully with 0.8-microm monodisperse colloidal silica beads utilizing a self-assembly packing technique. The silica packed chip substrate was covered and thermally press-bonded. After fabrication, the chip was filled with buffer solution by self-priming capillary action. The self-assembly packing at each channel served as a built-in nanofilter allowing quick loading of samples and running buffer solution without filtration. Because of a large surface area-to-volume ratio of the silica packing, reproducible control of electroosmotic flow was possible without leveling of the solutions in the reservoirs resulting 1.3% rsd in migration rate. The capillary electrophoretic separation characteristics of the chip were studied using fluorescein isothiocyanate (FITC)-derivatized amino acids as probe molecules. A mixture of FITC and four FITC-derivatized amino acids was successfully separated with 2-mm separation channel length.
authors.affiliation: Analytical Laboratory, Department of Chemistry, Konkuk University, 1 Hwayangdong, Gwangjingu, Seoul 143-701, Korea. chong.ahn@uc.edu
authors.forename: Jongman
authors.initials: J
authors.lastname: Park
authors.forename: Dami
authors.initials: D
authors.lastname: Lee
authors.forename: Won
authors.initials: W
authors.lastname: Kim
authors.forename: Shigeyoshi
authors.initials: S
authors.lastname: Horiike
authors.forename: Takahiro
authors.initials: T
authors.lastname: Nishimoto
authors.forename: Se Hwan
authors.initials: SH
authors.lastname: Lee
authors.forename: Chong H
authors.initials: CH
authors.lastname: Ahn
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D053801:Capillary Electrochromatography / Q000295:instrumentation / Q000379:methods*; D003102:Colloids; D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D003102:Colloids; D012822:Silicon Dioxide; D016650:Fluorescein-5-isothiocyanate