title: Demonstrating circulation in vasculogenic mimicry patterns of uveal melanoma by confocal indocyanine green angiography.
abstract: OBJECTIVE
  Vasculogenic mimicry patterns, formed by highly invasive melanoma cells, connect to endothelial cell-lined blood vessels and contain fluid in vitroand in vivo. This study was designed to determine if fluid leaks into vasculogenic mimicry patterns without circulation, or if fluid circulates in and clears from these patterns.
  
  
  METHODS
  Indocyanine green (ICG) laser scanning confocal angiography (Heidelberg Retinal Angiograph (HRA); Heidelberg Engineering, Heidelberg, Germany) was performed on nine patients with posterior choroidal melanoma in an institutional setting. Blood was drawn before the ICG injection and from the contralateral arm of the ICG injection site and 1 min after the injection. Outcome measures include time to first filling of retinal vessels and vasculogenic mimicry patterns and the time at which no fluorescence could be detected by the HRA instrument. After fluorescence was no longer detected in vessels or patterns, the tubes containing the patient's blood was imaged by the Heidelberg HRA.
  
  
  RESULTS
  Looping vasculogenic mimicry patterns were detected focally in five patients within 30 s after injection and were detectable up to 12 min post-injection. Blood drawn before ICG injection did not autofluoresce but ICG-containing blood pooled in the tube continued to fluoresce at 1-month post-injection.
  
  
  CONCLUSIONS
  Vasculogenic mimicry patterns are not part of the endothelial cell-lined vascular system and fluid enters these patterns through leakage. The rapid infusion of ICG into these patterns after injection and the disappearance of fluorescence detectable by the Heidelberg HRA suggest that fluid circulates in these patterns and does not accumulate as a stagnant pool.
authors.affiliation: Department of Ophthalmology, Hadassah-Hebrew University Medical Center, Jerusalem, Israel.
authors.forename: S
authors.initials: S
authors.lastname: Frenkel
authors.forename: I
authors.initials: I
authors.lastname: Barzel
authors.forename: J
authors.initials: J
authors.lastname: Levy
authors.forename: A Y
authors.initials: AY
authors.lastname: Lin
authors.forename: D-U
authors.initials: DU
authors.lastname: Bartsch
authors.forename: D
authors.initials: D
authors.lastname: Majumdar
authors.forename: R
authors.initials: R
authors.lastname: Folberg
authors.forename: J
authors.initials: J
authors.lastname: Pe'er
mesh_terms: D004730:Endothelium, Vascular / Q000473:pathology; D005451:Fluorescein Angiography; D006801:Humans; D007208:Indocyanine Green; D008545:Melanoma / Q000098:blood supply*; D008833:Microcirculation; D009389:Neovascularization, Pathologic / Q000473:pathology*; D014604:Uveal Neoplasms / Q000098:blood supply*
chemical_list: D007208:Indocyanine Green