Complete blood count, clinical chemistry, and serology profile by using a single tube of whole blood from mice.
Clinical pathology is a valuable means for assessing specific organ pathology and a screening tool for general animal health. Routine clinical pathology evaluation in mice usually includes whole blood for a complete blood count (CBC) and a clinical biochemistry analysis. Acquisition and analysis of these samples can be problematic due to the small volumes of blood that can be obtained from a mouse. Typically, a complete blood count requires blood from a tube containing an anticoagulant, whereas a clinical biochemistry profile needs blood from a serum clot tube. Because of the small volume that can be obtained, splitting the blood from a single mouse into 2 different tubes may result in inadequate samples to perform the desired tests or introduce inaccuracies. We explored the feasibility of using a single lithium heparin tube for generation of a CBC, biochemistry profile, and serology profile. We also evaluated the consistency of CBC data, including the quality of a peripheral blood smear taken from a lithium heparin or EDTA tube after various storage times. We found that CBC, biochemistry, and serology profiles could be obtained more readily when blood samples were placed in a single lithium heparin tube than in 2 separate tubes. In addition, the quality of blood smears and CBC results from the lithium heparin tube were comparable (with few exceptions) to those from an EDTA tube after prolonged storage.
Wiedmeyer
Charles E
CE
Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, Department of Veterinary Pathobiology, University of Missouri-Columbia, MO, USA. wiedmeyerc@missouri.edu
Ruben
Dawn
D
Franklin
Craig
C
D000818:Animals; D001772:Blood Cell Count / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D019963:Clinical Chemistry Tests / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D005260:Female; D007756:Laboratory Animal Science / Q000295:instrumentation / Q000379:methods; D008297:Male; D051379:Mice / Q000097:blood*; D008811:Mice, Inbred DBA; D012698:Serologic Tests / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*