[Rat dental papilla cell culture with nanometer-HAP in vitro].
OBJECTIVE
To determine the effects of nano-hydroxyapatite(nano-HAP)on the proliferation and activity of rat dental papilla cells(RDPCs)in vitro, and to evaluate the feasibility of using nano-hydroxyapatite(nano-HAP)as dental papilla cell scaffold in dental tissue engineering.


METHODS
RDPCs cultured with the porous nano-HAP in vitro served as the experimental group, and the routine culture of RDPCs in flasks served as the control. Scanning electronic microscope was used to observe the growth and adherence of the RDPCs to nano-HAP. Cell proliferation, cellular protein content, and alkaline phosphatase(ALP) were detected to assess the cellular activities.


RESULTS
RDPCs proliferated well, and adhered to the outer and inner surface of the nano-HAP scaffold. Compared with the control group, cells in the experimental group presented higher proliferation on 6 d and 8 d and higher cellular protein content on 6 d and 9 d. No significant difference was detected in the ALP activity in the 2 groups.


CONCLUSIONS
RDPCs seeded into nano-HAP grow better and have more vigorous cellular activity, suggesting that nano-HAP has excellent biocompatibility with dental papilla cells, and it can serve as a promising scaffold for dental tissue engineering.
Fang
Chang-Yun
CY
Department of Stomatology, Xiangya Hospital, Central South University, Changsha 410078, China.
Cao
Ying
Y
Xia
Yu
Y
Zhang
Xue-Mei
XM
Su
Zheng
Z
Li
Hui-Li
HL
D000818:Animals; D000831:Animals, Newborn; D001672:Biocompatible Materials / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003771:Dental Papilla / Q000166:cytology* / Q000648:ultrastructure; D017886:Durapatite / Q000494:pharmacology*; D008854:Microscopy, Electron; D053758:Nanoparticles; D036103:Nanotechnology; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; D017886:Durapatite