Interaction study of aspirin or clopidogrel on pharmacokinetics of donepezil hydrochloride in rats by HPLC-fluorescence detection.
The present study aims to investigate the possibility of interaction of aspirin (Asp) or clopidogrel (CG) on donepezil (DP) hydrochloride in rats by HPLC-fluorescence detection. The separation of DP was achieved in ca. 13 min without interference of Asp and CG on the chromatogram. DP levels in rat plasma with a single administration of DP (5 mg/kg, i.p., group I) and those with a co-administration of Asp (200 mg/kg, p.o., group II or 200 mg/kg, i.p., group III) or CG (5 mg/kg, p.o., group IV) were monitored. The DP concentrations determined in rat plasma ranged from 25.0 to 336.1 ng/mL. Pharmacokinetic parameters for these groups were calculated and compared with one another. No significant difference was observed on the comparison of group I with other groups except for the mean resident time of group IV (p = 0.012). These basic findings may help clinical inference when DP is co-administered with Asp and CG to human.
Wada
Mitsuhiro
M
Department of Clinical Pharmacy, Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
Nishiwaki
Junichiro
J
Yamane
Tomoko
T
Ohwaki
Yuichi
Y
Aboul-Enein
Hassan Y
HY
Nakashima
Kenichiro
K
D000284:Administration, Oral; D000818:Animals; D001241:Aspirin / Q000008:administration & dosage* / Q000097:blood; D002800:Cholinesterase Inhibitors / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D000077144:Clopidogrel; D000077265:Donepezil; D004347:Drug Interactions; D005453:Fluorescence; D007189:Indans / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics*; D007274:Injections, Intraperitoneal; D008297:Male; D015394:Molecular Structure; D010880:Piperidines / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D013988:Ticlopidine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood
D002800:Cholinesterase Inhibitors; D007189:Indans; D010880:Piperidines; D000077265:Donepezil; D000077144:Clopidogrel; D013988:Ticlopidine; D001241:Aspirin