title: Induction of apoptosis of osteoclasts by targeting transcription factors with decoy molecules.
abstract: We review the effects of two transcription factor decoy oligonucleotides on apoptosis of human osteoclasts (OCs). The first decoy molecule was designed to inhibit nuclear factor kappa-B (NF-kappaB) binding to target sequence, the second to increase estrogen receptor (ER) alpha expression. We found that both decoy molecules are potent inducers of apoptosis of human OCs, associated with increase of caspase 3 activity and decrease of interleukin 6 expression. In addition, we provide evidence indicating that these oligonucleotides are active in vivo in inducing OCs apoptosis. Because OCs are essential for skeletal development and remodeling throughout the life of animal and man, the approach described is of potential clinical importance.
authors.affiliation: Department of Biochemistry and Molecular Biology, Section of Molecular Biology, Università degli Studi di Ferrara, Via Fossato di Mortara 74, 44100 Ferrara, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Piva
authors.forename: Letizia
authors.initials: L
authors.lastname: Penolazzi
authors.forename: Margherita
authors.initials: M
authors.lastname: Zennaro
authors.forename: Ercolina
authors.initials: E
authors.lastname: Bianchini
authors.forename: Eros
authors.initials: E
authors.lastname: Magri
authors.forename: Monica
authors.initials: M
authors.lastname: Borgatti
authors.forename: Ilaria
authors.initials: I
authors.lastname: Lampronti
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Lambertini
authors.forename: Elisa
authors.initials: E
authors.lastname: Tavanti
authors.forename: Roberto
authors.initials: R
authors.lastname: Gambari
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D032701:Biomimetics; D016503:Drug Delivery Systems; D006801:Humans; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000494:pharmacology; D010010:Osteoclasts / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology*; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*
chemical_list: D009838:Oligodeoxyribonucleotides; D014157:Transcription Factors