title: Short-term biocompatibility of biphasic nanocolloids with potential use as anisotropic imaging probes.
abstract: Advances in nanotechnology, in particular the development of novel types of nanoparticles, will result in advanced tools for biomedical research and clinical practice. One exciting aspect of future nanomaterial research will be the possibility to combine therapy and imaging in multifunctional nanoparticle designs. In this context, anisotropic particles with subcellular dimensions may offer so far unattainable capabilities, because they could provide access to directional information with respect to nanoparticle-cell interactions. We have recently developed an electrified jetting process, which can produce water-stable polymer particles with two distinct phases. To address the first critical hurdle towards the application of these biphasic nanocolloids as imaging probes, short-term biocompatibility was evaluated using model cell culture systems. Exposure of human endothelial cells and murine fibroblasts to biphasic nanocolloids made of 0.5% polyacrylic acid and 4.5% poly(acrylamide-co-acrylic acid) did not affect cell proliferation as determined by a colorimetric proliferation assay. Moreover, double staining with Annexin V and propidium iodide and subsequent flow cytometric analysis indicated high cell viability, although slightly decreased viability was observed at the highest dose tested (1mg particles/10(6) seeded cells). Particle internalization as well as surface binding occurred simultaneously for both cell types, as evidenced by confocal laser scanning microscopy. Taken together, these results suggest excellent short-term biocompatibility in physiological systems for wide concentration ranges of the biphasic nanocolloids and open possibilities for future work investigating receptor- or surface marker-mediated targeting.
authors.affiliation: Department of Chemical Engineering, University of Michigan, 2300 Hayward Street, Ann Arbor, MI 48109, USA.
authors.forename: Mutsumi
authors.initials: M
authors.lastname: Yoshida
authors.forename: Kyung-Ho
authors.initials: KH
authors.lastname: Roh
authors.forename: Joerg
authors.initials: J
authors.lastname: Lahann
mesh_terms: D000178:Acrylamides / Q000737:chemistry; D000180:Acrylic Resins / Q000737:chemistry; D000818:Animals; D016880:Anisotropy; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003102:Colloids / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D003952:Diagnostic Imaging / Q000379:methods*; D004705:Endocytosis / Q000187:drug effects; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006801:Humans; D008422:Materials Testing / Q000379:methods*; D051379:Mice; D008856:Microscopy, Fluorescence; D041681:NIH 3T3 Cells; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size
chemical_list: D000178:Acrylamides; D000180:Acrylic Resins; D003102:Colloids; D005456:Fluorescent Dyes; C431184:poly(acrylamide-co-acrylic acid); C006903:carbopol 940; D005954:Glucosephosphate Dehydrogenase