title: In vitro evaluation of magnetic resonance imaging at 3.0 tesla on clonogenic ability, proliferation, and cell cycle in human embryonic lung fibroblasts.
abstract: OBJECTIVE
  We investigated the influence of magnetic resonance (MR) at 3.0 T on clonogenic ability, proliferation, and cell cycle in an embryonic human cell line.
  
  
  METHODS
  Cells (human lung fibroblasts Hel 299) were exposed to the static magnetic field (3.0 T) of a magnetic resonance imager (MRI) and to a turbo spin echo sequence at 3.0 T within clinical limitations (specific absorption rate 0.92 W/kg). A special MR-compatible incubation system was used. A control group (sham-exposed) and a MRI group (exposed) were set up. We investigated 3 biologic endpoints: colony forming, cell cycle, and proliferation ability. The exposure time was 2 hours in each experiment.
  
  
  RESULTS
  In the statistical analysis, none of these tests showed relevant differences between the exposed and sham-exposed group.
  
  
  CONCLUSIONS
  No influences of the static field alone as well as a turbo spin echo sequence at 3.0 T on clonogenic ability, proliferation, or cell cycle in eugenic human lung fibroblasts were found.
authors.affiliation: Department of Diagnostic Radiology, Eberhard-Karls University, Tübingen, Germany. nina.schwenzer@med.uni-tuebingen.de
authors.forename: Nina F
authors.initials: NF
authors.lastname: Schwenzer
authors.forename: Rüdiger
authors.initials: R
authors.lastname: Bantleon
authors.forename: Brigitte
authors.initials: B
authors.lastname: Maurer
authors.forename: Rainer
authors.initials: R
authors.lastname: Kehlbach
authors.forename: Tina
authors.initials: T
authors.lastname: Herberts
authors.forename: Claus D
authors.initials: CD
authors.lastname: Claussen
authors.forename: Enno
authors.initials: E
authors.lastname: Rodegerdts
mesh_terms: D002453:Cell Cycle / Q000502:physiology*; D049109:Cell Proliferation; D004574:Electromagnetic Fields / Q000009:adverse effects*; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D008168:Lung / Q000502:physiology*; D008279:Magnetic Resonance Imaging / Q000009:adverse effects / Q000295:instrumentation*; D013234:Stem Cells