[Study of cryopreservation of differentiated hepatocyte derived from human bone marrow stem cells].
OBJECTIVE
To observe the viability and function of human bone marrow stem cell-derived hepatocytes following cryopreservation in vitro.


METHODS
Human bone marrow cells were induced to differentiate into hepatocytes in the presence of multiple factors. Mature hepatocytes were cryopreserved in 90% FBS and 10% DMSO (Group A), 10% FBS, 30% glycerol and 60% conditioned medium (Group B), and 10% FBS, 10% DMSO, and 80% UW solution (Group C). The cells were thawed after 4 weeks, and the cell viability and the albumin level were determined.


RESULTS
The human bone marrow derived hepatocytes maintained functional morphology after thawing. The viabilities in Group A, B and C were (60.0 +/- 3.3)%, (91.0 +/- 2.6)%, and (89.0 +/- 1.4)%, respectively. After culturing for 24 h, the albumin levels in Group A, B and C were (0.210 +/- 0.005) g/L, (0.340 +/- 0.020) g/L and (0.330 +/- 0.030) g/L, respectively.


CONCLUSIONS
Human bone marrow stem cell-derived hepatocytes can maintain the viability and function after cryopreservation. These cells may contribute to an important source of hepatocytes for clinical hepatocyte transplantation and artificial liver support system.
Qu
Qiang
Q
Department of General Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.
Li
Bing-Lu
BL
Zhao
Yu-Pei
YP
Chen
Cui-Zhu
CZ
Yan
Xu
X
Wang
Lei
L
Zhou
Li
L
He
Xiao-Dong
XD
D000328:Adult; D001854:Bone Marrow Cells / Q000166:cytology* / Q000502:physiology; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D022781:Hepatocytes / Q000166:cytology* / Q000502:physiology / Q000637:transplantation; D006801:Humans; D023461:Myeloid Progenitor Cells / Q000166:cytology* / Q000502:physiology
D003451:Cryoprotective Agents