title: Assessment of the solid-state composition of an active salicylanilide compound by FT-Raman spectroscopy.
abstract: A biologically active salicylanilide compound currently appears in three known solid-state forms: polymorph I (Pol I), polymorph II (Pol II) and the amorphous form (Amorph). The obtained FT-Raman spectra revealed several regions of interest (ROIs) qualitatively distinguishing the different forms, allowing samples with an unknown polymorphic composition to be quantitatively analysed by FT-Raman spectroscopy. The Markov-transformed peak areas of the Raman-bands in the ROIs from the samples were determined and compared with the transformed peak areas obtained for the reference solid-state forms. A constrainted linear regression model estimated the contribution of each reference to the different samples. The applicability of this approach was demonstrated by analysing commercially available batches.
authors.affiliation: Drug Quality & Registration (DruQuaR) Group, Department of Pharmaceutical Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, B-9000 Gent, Belgium. bart.despiegeleer@ugent.be
authors.forename: B
authors.initials: B
authors.lastname: De Spiegeleer
authors.forename: B
authors.initials: B
authors.lastname: Baert
authors.forename: N
authors.initials: N
authors.lastname: Diericx
authors.forename: D
authors.initials: D
authors.lastname: Seghers
authors.forename: F
authors.initials: F
authors.lastname: Verpoort
authors.forename: L
authors.initials: L
authors.lastname: Van Vooren
authors.forename: C
authors.initials: C
authors.lastname: Burvenich
authors.forename: G
authors.initials: G
authors.lastname: Slegers
mesh_terms: D008390:Markov Chains; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000737:chemistry*; D012016:Reference Values; D012458:Salicylanilides / Q000032:analysis*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D004364:Pharmaceutical Preparations; D012458:Salicylanilides; C034596:salicylanilide