[Effect of Qingdu Suppository-containing serum on cervical carcinoma SiHa cell MDM2 gene expression].
OBJECTIVE
To investigate the effect and mechanism of Qingdu Suppository (QDS) in suppressing the growth of SiHa cell of cervical carcinoma on the cytobiologic and molecular biologic level.


METHODS
SiHa cells were cultured in media with serum containing different concentrations of QDS and blank serum, as well as serum containing various Chinese herbal or Western drugs as positive control. SiHa cell growth and proliferation was determined by MTT assay, the cell cycle and apoptosis were analyzed by flow cytometry technique, and the expression of proto-oncogene MDM2 detected semi-quantitatively by RT-PCR.


RESULTS
The amount of cells decreased after cultured with drug-containing serum 8% in concentration, with the highest inhibitory effect shown by drug-containing serum after acting for 72 h, which was significantly different to that in the control group (P < 0.01). Among the various time phases of cell cycle, the percentage of cells in S phase was significantly decreased. Drug-containing serum showed somewhat effect on cell apoptosis, but no obvious regularity could be found. RT-PCR showed significant decrease of MDM2 expression.


CONCLUSIONS
QDS containing serum could not only directly suppress the growth of cervical carcinoma cells, but also inhibit the tumor by the molecular mechanism of down-regulating MDM2 gene expression.
Li
Yun-Bo
YB
Department of Gynecology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing.
Jin
Zhe
Z
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D000090063:Proto-Oncogene Mas; D051736:Proto-Oncogene Proteins c-mdm2 / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D044967:Serum; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000473:pathology
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; C528245:qingdu; C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2