title: Heritable skewed X-chromosome inactivation leads to haemophilia A expression in heterozygous females.
abstract: Factor VIII gene, F8, mutations cause haemophilia A (HA), an X-linked recessive disorder. Expression in heterozygous females has been ascribed to skewed X-chromosome inactivation (XCI). To investigate the cause of HA in three heterozygous females within an Atlantic Canadian kindred, the proband (severely affected girl, FVIII activity: 2%) and 17 relatives across three generations were studied. F8 genotype, FVIII activity, XCI ratio (XCIR) (paternal active X: maternal active X), karyotype, submegabase resolution tiling set array competitive genome hybridization (competitive genomic hybridization (SMRT)), and microsatellite analyses were utilized. A positive linear relationship between FVIII activity and percentage-activated normal X-chromosome was found in HA heterozygous females (R(2)=0.87). All affected, but no unaffected females, had an XCIR skewed toward activation of the mutant X-chromosome (proband 92:8, SD 2). Unexpectedly, high numbers of females have dramatically skewed XCIRs (>80:20 or <20:80) (P<0.05). The distribution of XCIR frequencies within this family was significantly different than predicted by normal population data or models of random XCI (P<0.025), with more females having higher degrees of skewing. Known causes of skewing, such as chromosomal abnormalities, selection against deleterious alleles, and X-inactive-specific transcript mutations, are not consistent with our results. This study shows that FVIII activity in HA heterozygous females can be directly related to XCI skewing, and that low FVIII activity in females in this family is due to unfavourable XCI skewing. Further, the findings suggest that these XCI ratios are genetically influenced, consistent with a novel heritable human X controlling element (XCE) functioning similarly to the mouse Xce.
authors.affiliation: Department of Pathology, Dalhousie University, Halifax, Nova Scotia, Canada.
authors.forename: Nisa K
authors.initials: NK
authors.lastname: Renault
authors.forename: Sarah
authors.initials: S
authors.lastname: Dyack
authors.forename: Melanie J
authors.initials: MJ
authors.lastname: Dobson
authors.forename: Teresa
authors.initials: T
authors.lastname: Costa
authors.forename: Wan L
authors.initials: WL
authors.lastname: Lam
authors.forename: Wenda L
authors.initials: WL
authors.lastname: Greer
mesh_terms: D002675:Child, Preschool; D007446:Chromosome Inversion; D041321:Chromosomes, Human, X / Q000235:genetics; D005169:Factor VIII / Q000235:genetics* / Q000378:metabolism; D005260:Female; D008040:Genetic Linkage; D006467:Hemophilia A / Q000235:genetics*; D006579:Heterozygote; D006801:Humans; D007223:Infant; D008297:Male; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D010375:Pedigree; D049951:X Chromosome Inactivation / Q000235:genetics*
chemical_list: D005169:Factor VIII