A trinuclear copper(II) complex of 2,4,6-tris(di-2-pyridylamine)-1,3,5-triazine shows prominent DNA cleavage activity.
A highly water soluble 3:2 complex of copper(II) and 2,4,6-tris(di-2-pyridylamine)-1,3,5-triazine (TDAT) has been synthesized and structurally characterized. The complex crystallized in a triclinic P1 space group with a molecular formula of [Cu3(TDAT)2Cl3]Cl3.2H2O (1), where each copper ion is coordinated by four pyridine nitrogen atoms and an apical chloride. The trinuclear complex is stable at physiological relevant conditions. It can bind to DNA through electrostatic attraction and cleave efficiently the supercoiled pBR322 DNA into its nicked and linear forms at micromolar concentrations. Active oxygen intermediates such as hydroxyl radicals and singlet oxygen generated in the presence of 1 may act as active species for the DNA scission.
Chen
Jingwen
J
State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
Wang
Xiaoyong
X
Shao
Ying
Y
Zhu
Jianhui
J
Zhu
Yangguang
Y
Li
Yizhi
Y
Xu
Qiang
Q
Guo
Zijian
Z
D000631:Aminopyridines / Q000737:chemistry*; D003300:Copper / Q000737:chemistry* / Q000378:metabolism; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D008968:Molecular Conformation; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000737:chemistry; D014227:Triazines / Q000737:chemistry*
C519945:2,4,6-tris(di-2-pyridylamine)-1,3,5-triazine; D000631:Aminopyridines; D009942:Organometallic Compounds; D017382:Reactive Oxygen Species; D014227:Triazines; D003300:Copper; D004247:DNA