[Modulation of cyclosporin a on the expression of MMP-9 AND MMP-2 of the first-trimester human trophoblast cells].
The aim of this study was to explore whether cyclosporine A (CsA) can modulate expression of MMP-9 and MMP-2 and invasion of the first-trimester human trophoblast cells. The invasive ability of the trophoblast cells modulated by CsA was observed by Matrigel invasion assay. The effect of CsA on the transcription and translation of MMP-9 and MMP-2 was determined by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and Gelatin Zymography. In-cell Western was used to detect the level of phospho-ERK1/2 of trophoblast cells treated with CsA. We found that CsA(1.0 micromol/L) could remarkably promote the invasive ability of the first-trimester human trophoblast cells; CsA(1.0 micromol/L) could also induce the expression of MMP-9 and MMP-2 at transcriptional and translational level; U0126,a MEK inhibitor,could obviously inhibit the enhanced invasion and expression of MMP-9 and MMP-2 of these cells induced by CsA; CsA (1.0 micromol/L) could activate the ERKl/2 in a time-dependent manner. These results above indicate that CsA can induce the expression of MMP-9 and MMP-2 through activating MAPK/ERK1/2, which contributes to the improved invasion of the first-trimester human trophoblast cells.
Zhou
Wen Hui
WH
Laboratory for Reproductive Immunology, Hospital & Institute of Obstetrics and Gynecology, Shanghai Medical College, Fudan University, Shanghai 200011.
Du Mei
Rong
R
Dong
Lin
L
Zhu
Xiao Yong
XY
He
Yin Yan
YY
Da Li
Jin
J
D015153:Blotting, Western; D002478:Cells, Cultured; D016572:Cyclosporine / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014327:Trophoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D007166:Immunosuppressive Agents; D016572:Cyclosporine; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9