title: Immunoisolating pancreatic islets by encapsulation with selective withdrawal.
abstract: This manuscript reports the application of the selective-withdrawal coating technique to the microencapsulation of insulin-producing pancreatic islets within thin poly(ethylene glycol) coatings. These polymer coatings permit the islets to respond to changes in glucose concentration by producing insulin with a dose-response profile that is substantially similar to that of unencapsulated islets. Furthermore, the hydrogel capsules exclude the large molecules of the immune system. These results suggest that the microencapsulation technique-which combines droplet formation from a flow of two immiscible fluids with polymerization chemistries-has the characteristics required for the transplantation of islets for the treatment of Type I diabetes.
authors.affiliation: The James Franck Institute and Department of Physics, The University of Chicago, Chicago, IL 60637, USA.
authors.forename: Jason L
authors.initials: JL
authors.lastname: Wyman
authors.forename: Seda
authors.initials: S
authors.lastname: Kizilel
authors.forename: Ryan
authors.initials: R
authors.lastname: Skarbek
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Zhao
authors.forename: Matthew
authors.initials: M
authors.lastname: Connors
authors.forename: William S
authors.initials: WS
authors.lastname: Dillmore
authors.forename: William L
authors.initials: WL
authors.lastname: Murphy
authors.forename: Milan
authors.initials: M
authors.lastname: Mrksich
authors.forename: Sidney R
authors.initials: SR
authors.lastname: Nagel
authors.forename: Marc R
authors.initials: MR
authors.lastname: Garfinkel
mesh_terms: D000818:Animals; D002214:Capsules / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003922:Diabetes Mellitus, Type 1 / Q000628:therapy*; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D007107:Immune System; D007515:Islets of Langerhans / Q000276:immunology* / Q000378:metabolism*; D016381:Islets of Langerhans Transplantation / Q000295:instrumentation* / Q000379:methods*; D037102:Lectins / Q000737:chemistry; D018613:Microscopy, Confocal; D008970:Molecular Weight; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D013552:Swine
chemical_list: D002214:Capsules; D020099:Coated Materials, Biocompatible; D020100:Hydrogels; D037102:Lectins; D011108:Polymers; D011092:Polyethylene Glycols