New process for the manufacture of alpha-1 antitrypsin.
We report a novel method for the extraction of alpha-1 antitrypsin (AAT) from plasma. This method uses an anion-exchange column and two metal chelate columns. The AAT is recovered in a 60% yield and a purity of over 90%. The AAT manufactured by this method was stable at 4 degrees C for 12 months without any stabilisers. Other proteins of therapeutic value and commercial interest are recoverable in good yields. The simplicity of this process makes it a suitable alternative to the traditionally employed ethanol precipitation method where high ethanol levels inactivate AAT.
Kumpalume
Peter
P
Bio Products Laboratory, Dagger Lane, Elstree, Herts. WD6 3BX, UK. pkumpalume@gmail.com
Podmore
Adrian
A
LePage
Christopher
C
Dalton
Joan
J
D000818:Animals; D002847:Chromatography, Agarose; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors / Q000378:metabolism; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D013552:Swine; D000515:alpha 1-Antitrypsin / Q000097:blood / Q000302:isolation & purification* / Q000494:pharmacology
D012997:Solvents; D000515:alpha 1-Antitrypsin; D010196:Pancreatic Elastase