title: Evidence for the existence of satellite DNA-containing connection between metaphase chromosomes.
abstract: Physical connections between mitotic chromosomes have been reported previously. It was assumed that the interchromosome connection was based on the DNA-protein thread. However, the data about DNA sequences and protein component in the thread is fragmentary. We demonstrated on the mouse cultured cell line and prematurely condensed chromosomes that: (a) all four mouse satellite DNA fragments (major and minor satellite, mouse satellite 3 (MS3) and mouse satellite 4 (MS4)) were involved in the thread formation; (b) MS4 was involved in the thread to the least extent among all the other fragments; (c) telomere was never a member of the thread; (d) the thread was synthesized at a late G(2) phase; (e) RNA helicase p68 and CENP-B were among the protein components of the interchromosome connection. It was shown by FACS analysis that in mouse and human cell lines: (1) the flow karyotype spectrums were never free from chromosome aggregates; (2) chromosome association did not depend on the chromosome length and each chromosome was free to associate with the other.
authors.affiliation: Institute of Cytology RAS, Tikhoretsky Avenue 4, St Petersburg, Russia.
authors.forename: I S
authors.initials: IS
authors.lastname: Kuznetsova
authors.forename: N I
authors.initials: NI
authors.lastname: Enukashvily
authors.forename: E M
authors.initials: EM
authors.lastname: Noniashvili
authors.forename: A N
authors.initials: AN
authors.lastname: Shatrova
authors.forename: N D
authors.initials: ND
authors.lastname: Aksenov
authors.forename: V V
authors.initials: VV
authors.lastname: Zenin
authors.forename: A P
authors.initials: AP
authors.lastname: Dyban
authors.forename: O I
authors.initials: OI
authors.lastname: Podgornaya
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000235:genetics; D002460:Cell Line; D045744:Cell Line, Tumor; D051936:Centromere Protein B / Q000378:metabolism; D002875:Chromosomes / Q000235:genetics* / Q000378:metabolism; D053487:DEAD-box RNA Helicases / Q000378:metabolism; D004276:DNA, Satellite / Q000235:genetics* / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008677:Metaphase / Q000235:genetics*; D051379:Mice; D008938:Mitosis / Q000235:genetics; D016615:Telomere / Q000235:genetics / Q000378:metabolism
chemical_list: D051936:Centromere Protein B; D004276:DNA, Satellite; D053487:DEAD-box RNA Helicases