title: Characterization of poly(ethylene glycol)-modified bovine hemoglobin by capillary zone electrophoresis.
abstract: In this study capillary zone electrophoresis (CZE) was used to analyze the poly(ethylene glycol)-modified bovine hemoglobin(PEG-bHb). The results show that CZE separated the subunit of bovine hemoglobin based on the number of PEG conjugating to the protein surface, which makes it possible to evaluate the degree of modification of hemoglobin subunit; meanwhile, it also reflected the stability of PEG attaching to hemoglobin after incubating with hydroxylamine, which makes it successful to detect the distribution of attachment site and evaluate the stability of PEG on the surface of hemoglobin. As a simple, fast and accurate method, CZE is suitable to monitor the production procedures and quality control of the final products of the PEG-bHb.
authors.affiliation: Beijing Kaizheng Biotech Developing Ltd., Beijing, P.R. China.
authors.forename: Xian-Zhong
authors.initials: XZ
authors.lastname: Li
authors.forename: Qin-Hong
authors.initials: QH
authors.lastname: Yang
authors.forename: Jiu-Feng
authors.initials: JF
authors.lastname: Yan
authors.forename: Ke
authors.initials: K
authors.lastname: Xu
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: Xue-Ying
authors.initials: XY
authors.lastname: He
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Zhang
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
mesh_terms: D000818:Animals; D001802:Blood Substitutes / Q000138:chemical synthesis / Q000737:chemistry*; D002417:Cattle; D004355:Drug Stability; D019075:Electrophoresis, Capillary / Q000379:methods*; D006454:Hemoglobins / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D021122:Protein Subunits / Q000737:chemistry; D011786:Quality Control
chemical_list: D001802:Blood Substitutes; D006454:Hemoglobins; D021122:Protein Subunits; D011092:Polyethylene Glycols