Solvent-free synthesis of glyceryl ferulate using a commercial microbial lipase.
A process was optimized for the enzymatic synthesis of glyceryl ferulate with a yield of up to 96% using a vacuum-rotary evaporation strategy under following conditions: 15 mmol glycerol, 1.5 mmol ethyl ferulate, 170 mg Candida antarctica lipase, at 60 degrees C for 10 h and under a vacuum of 10 mm Hg. The immobilized lipase can be used 10 times.
Sun
Shangde
S
School of Food Science and Technology, Southern Yangtze University, The Key Laboratory of Food Science and Safety, Ministry of Education, Wuxi, Jiangsu, PR China.
Shan
Liang
L
Jin
Qingzhe
Q
Liu
Yuanfa
Y
Wang
Xingguo
X
D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000737:chemistry / Q000378:metabolism*; D004951:Esterification; D005656:Fungal Proteins; D005990:Glycerol / Q000737:chemistry*; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D015394:Molecular Structure; D012997:Solvents / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013696:Temperature
D003373:Coumaric Acids; D005656:Fungal Proteins; D012997:Solvents; C004999:ferulic acid; D008049:Lipase; C431349:lipase B, Candida antarctica; D005990:Glycerol