Trophic factor supplemented UW solution reduces intimal hyperplasia in the rat aortic transplant model.
BACKGROUND
Chronic allograft nephropathy (CAN) is associated with delayed graft function, cold ischemic injury, and is an important cause of premature graft loss. A characteristic vascular lesion of CAN is intimal hyperplasia (IH). The goal of this study was to evaluate the effect of cold storage in University of Wisconsin solution supplemented with trophic factors (UW-TF) on IH in rat aortic isograft (IG) and allograft (AG) models.


METHODS
F344 --> F344 and Lewis --> F344 orthotopic abdominal aortic transplants were performed after 48 h of cold storage in either UW or UW-TF solution with and without immunosuppression.


RESULTS
Significant reduction in IH was observed when IG were stored in UW-TF solution compared to UW solution. A significant reduction in intimal inflammation was observed in UW-TF stored, nonimmunosuppressed AG. In immunosuppressed recipients, AG stored in UW-TF solution evidenced significantly less IH compared to those stored in UW alone.


CONCLUSIONS
UW-TF solution decreased IH in both alloindependent and dependent models.
Schmiedt
Chad W
CW
Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 2015 Linden Drive, Madison, WI 57306-1102, USA.
Schwab
Michelle C
MC
Dubielzig
Richard R
RR
Murphy
Christopher J
CJ
McAnulty
Jonathan F
JF
D000818:Animals; D001011:Aorta / Q000187:drug effects*; D050377:Cold Ischemia / Q000009:adverse effects; D006965:Hyperplasia / Q000517:prevention & control*; D007165:Immunosuppression Therapy; D008297:Male; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D014185:Transplantation, Isogeneic; D017539:Tunica Intima / Q000187:drug effects*
D019188:Organ Preservation Solutions