[Efficacy of a kaurenic acid extracted from the Venezuelan plant Wedelia trilobata (Asteracea) against Leishmania (Viannia) braziliensis].
BACKGROUND
Leishmaniasis is a global public health problem without adequate treatment options, hence requiring research on new drug development.


OBJECTIVE
Our main objective was the evaluation of a kaurenic acid (ent-kaur-16-in-19-oico), isolated from the Venezuelan plant Wedelia trilobata (Asteracea), on Leishmania (V) braziliensis both in vivo and in vitro.


METHODS
The in vitro assay was performed using axenic amastigotes and promastigotes as well as infected and uninfected macrophages. Parasites and macrophages were treated with concentrations of the compound varying between 10 and 250 microg/ml. The effect of the compound on cellular viability was evaluated by counting dead and living cells in a hemocytometer and by the colorimetric method using MTT (Methylthiazoletetrazolium). For the in vivo assay, Balb/c mice were infected subcutaneously with 1 x 10(6) promastigotes of L. (V.) braziliensis and afterwards treated with a daily dose of 30 mg/kg in 100 microl of kaurenic acid administered by intraperitoneal injection for one week.


RESULTS
The compound had a lethal effect on axenic amastigotes and promastigotes with DL50 of 0.25 and 0.78 microg/ml, respectively, in 24 hours. Low toxicity was observed for J774-G8 macrophages with a DL50 of 25 microg/ml and high viability (70-92%), while a moderate viability was observed for infected macrophages (37-81%), with concentrations of 25 microg/ml or less. Additionally, a 70% reduction was observed in the size of the skin lesions in Balb/c mice with no evident toxic effect.


CONCLUSIONS
The results indicate that this compound has a potent leishmanicidal effect on L. (V.) braziliensis.
Brito
Solanny
S
Universidad de Oriente, Cumaná, Estado Sucre, Venezuela.
Crescente
Oscar
O
Fernández
Alexis
A
Coronado
Aura
A
Rodriguez
Noris
N
D000818:Animals; D004224:Diterpenes / Q000494:pharmacology; D005260:Female; D007892:Leishmania braziliensis / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000494:pharmacology*; D031209:Wedelia
D004224:Diterpenes; D010936:Plant Extracts; C014341:argyrophilic acid