title: Expression profiling of peroxisome proliferator-activated receptor-delta (PPAR-delta) in mouse tissues using tissue microarray.
abstract: Peroxisome proliferator-activated receptor-delta (PPAR-delta) is known as a transcription factor involved in the regulation of fatty acid oxidation and mitochondrial biogenesis in several tissues, such as skeletal muscle, liver and adipose tissues. In this study, to elucidate systemic physiological functions of PPAR-delta, we examined the tissue distribution and localization of PPAR-delta in adult mouse tissues using tissue microarray (TMA)-based immunohistochemistry. PPAR-delta positive signals were observed on variety of tissues/cells in multiple systems including cardiovascular, urinary, respiratory, digestive, endocrine, nervous, hematopoietic, immune, musculoskeletal, sensory and reproductive organ systems. In these organs, PPAR-delta immunoreactivity was generally localized on the nucleus, although cytoplasmic localization was observed on several cell types including neurons in the nervous system and cells of the islet of Langerhans. These expression profiling data implicate various physiological roles of PPAR-delta in multiple organ systems. TMA-based immunohistochemistry enables to profile comprehensive protein localization and distribution in a high-throughput manner.
authors.affiliation: Pharmacology Department, Tsukuba Research Laboratories and Nuclear Receptor Discovery Research, High Throughput Biology, Discovery Research, GlaxoSmithKline, 43 Wadai, 300-4247, Tsukuba, Ibaraki, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Higashiyama
authors.forename: Andrew N
authors.initials: AN
authors.lastname: Billin
authors.forename: Yuji
authors.initials: Y
authors.lastname: Okamoto
authors.forename: Mine
authors.initials: M
authors.lastname: Kinoshita
authors.forename: Satoshi
authors.initials: S
authors.lastname: Asano
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D002319:Cardiovascular System / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D002467:Cell Nucleus / Q000737:chemistry / Q000378:metabolism; D003593:Cytoplasm / Q000737:chemistry / Q000378:metabolism; D004064:Digestive System / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D004703:Endocrine System / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D005260:Female; D006413:Hematopoietic System / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D007107:Immune System / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D046228:Microarray Analysis / Q000379:methods*; D009141:Musculoskeletal System / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D009420:Nervous System / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D047494:PPAR delta / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D012137:Respiratory System / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D012679:Sense Organs / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D014566:Urogenital System / Q000737:chemistry / Q000166:cytology / Q000378:metabolism
chemical_list: D000906:Antibodies; D047494:PPAR delta