Characterization of surface adhesins of lactobacilli used in production of probiotic preparations.
We examined production of the protein-lipoteichoic complex detectable by concanavalin A in six lactobacillus strains. A correlation was found between detection of this complex by both reaction with concanavalin A and agglutination with strain-specific antiserum. The cultures were characterized by expression of different types of adhesins. Among them, strains were differentiated with low adhesion activity and intensive production of the protein-lipoteichoic complex and strains with wide adhesin spectrum not producing the complex. We assume that a combination of lactobacillus variants differing in production of the protein-lipoteichoic complex and adhesion potency can be a basis of efficient probiotic preparation.
Anokhina
I V
IV
Department of Microbiology, Russian University of Peoples' Friendship, Moscow.
Kravtsov
E G
EG
Yashina
N V
NV
Ermolaev
A V
AV
Chesnokova
V L
VL
Dalin
M V
MV
D018829:Adhesins, Bacterial / Q000302:isolation & purification*; D000372:Agglutination Tests; D001422:Bacterial Adhesion / Q000502:physiology; D003208:Concanavalin A; D007778:Lactobacillus / Q000737:chemistry*; D046912:Multiprotein Complexes / Q000378:metabolism; D019936:Probiotics / Q000737:chemistry*; D011956:Receptors, Cell Surface / Q000378:metabolism; D013045:Species Specificity
D018829:Adhesins, Bacterial; D046912:Multiprotein Complexes; D011956:Receptors, Cell Surface; C081954:lipoteichoic acid receptor; D003208:Concanavalin A