The transcriptional repressor Glis2 is a novel binding partner for p120 catenin.
In epithelial cells, p120 catenin (p120) localizes at cell-cell contacts and regulates adhesive function of the cadherin complex. In addition, p120 has been reported to localize in the nucleus, although the nuclear function of p120 is not fully understood. Here, we report the identification of Gli-similar 2 (Glis2) as a novel binding protein for p120. Glis2 is a Krüppel-like transcriptional repressor with homology to the Gli family, but its physiological function has not been well characterized. In this study, we show that coexpression of Glis2 and Src induces nuclear translocation of p120. Furthermore, p120 induces the C-terminal cleavage of Glis2, and this cleavage is further enhanced by Src. The cleaved form of Glis2 loses one of its five zinc finger domains, but it is still able to bind DNA. Functional studies in chick neural tube indicate that full-length Glis2 can affect neuronal differentiation, whereas the cleaved form requires coexpression of p120 to have a similar effect. These data indicate that p120 has additional novel functions in the nucleus together with Glis2.
Hosking
Catherine Rose
CR
Medical Research Council Laboratory for Molecular Cell Biology and Cell Biology Unit, Department of Biology, University College London, London WC1E 6BT, UK.
Ulloa
Fausto
F
Hogan
Catherine
C
Ferber
Emma C
EC
Figueroa
Angélica
A
Gevaert
Kris
K
Birchmeier
Walter
W
Briscoe
James
J
Fujita
Yasuyuki
Y
D021581:Active Transport, Cell Nucleus; D000818:Animals; D019556:COS Cells; D051177:Catenins; D002417:Cattle; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D002460:Cell Line; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D004247:DNA / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D051741:Kruppel-Like Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D034622:RNA Interference; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D016335:Zinc Fingers; D019061:src-Family Kinases / Q000378:metabolism; D000095002:Delta Catenin
D051177:Catenins; D015815:Cell Adhesion Molecules; C447919:GLIS2 protein, human; D051741:Kruppel-Like Transcription Factors; D010750:Phosphoproteins; D011993:Recombinant Fusion Proteins; D004247:DNA; D019061:src-Family Kinases; D000095002:Delta Catenin