Pseudomonas fluorescens SBW25 biofilm and planktonic cells have differentiable Raman spectral profiles.
Biofilms, and other bacterial aggregations, are of significance in both environmental microbiology and in plant and human pathogenesis. Comparative single-cell Raman spectral analysis can differentiate between planktonic bacteria and those recovered from biofilms and appears to offer a new means by which to investigate bacterial cell physiology, metabolic status, and stress under different environmental conditions.
Huang
Wei E
WE
Molecular Microbiology Ecology Section, CEH-Oxford, Mansfield Road, Oxford, OX1 3SR, UK.
Ude
Susanne
S
Spiers
Andrew J
AJ
D001557:Benzenesulfonates; D018441:Biofilms; D016002:Discriminant Analysis; D004783:Environmental Microbiology; D005456:Fluorescent Dyes; D010933:Plankton; D025341:Principal Component Analysis; D011551:Pseudomonas fluorescens / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification / Q000502:physiology; D013059:Spectrum Analysis, Raman / Q000379:methods*
D001557:Benzenesulfonates; D005456:Fluorescent Dyes; C007061:C.I. Fluorescent Brightening Agent 28