title: Notch signaling inhibits growth of the human lung adenocarcinoma cell line A549.
abstract: In lung cancers the Notch signaling may function as an oncogene or a tumor suppressor depending on the tumor cell types. In this study we examined the expression of Notch receptors in the human lung adenocarcinoma cell lines A549 and SPC-A-1. We over-expressed the active form of Notch1 (NIC) in A549 cells by constitutive transfection to evaluate the effects of the Notch signaling on lung adenocarcinoma cells. Our results showed that over-expression of NIC in A549 cells inhibited the growth of A549 cells through induction of cell cycle arrest. Moreover, over-expression of NIC inhibited the colony-forming activity of A549 cells when cultured in methylcellulose medium, and their ability to form tumors in nude mice. These data suggest that the Notch signaling may function as a tumor inhibitor in human lung adenocarcinoma cells.
authors.affiliation: State Key Laboratory of Cancer Biology, Department of Medical Genetics and Developmental Biology, Xijing Hospital, Fourth Military Medical University, Xian 710032, P.R. China.
authors.forename: Qijun
authors.initials: Q
authors.lastname: Zheng
authors.forename: Hongyan
authors.initials: H
authors.lastname: Qin
authors.forename: Hongwei
authors.initials: H
authors.lastname: Zhang
authors.forename: Junchang
authors.initials: J
authors.lastname: Li
authors.forename: Lihong
authors.initials: L
authors.lastname: Hou
authors.forename: Hongbin
authors.initials: H
authors.lastname: Wang
authors.forename: Xueping
authors.initials: X
authors.lastname: Zhang
authors.forename: Suzhen
authors.initials: S
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Feng
authors.forename: Yingmin
authors.initials: Y
authors.lastname: Liang
authors.forename: Hua
authors.initials: H
authors.lastname: Han
authors.forename: Dinghua
authors.initials: D
authors.lastname: Yi
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D051880:Receptors, Notch; D025521:Tumor Suppressor Proteins