title: Monolayers assembled from a glycolipid biosurfactant from Pseudozyma (Candida) antarctica serve as a high-affinity ligand system for immunoglobulin G and M.
abstract: A carbohydrate ligand system has been developed which is composed of self-assembled monolayers (SAMs) of mannosylerythritol lipid-A (MEL-A) from Pseudozyma antarctica, serving for human immunoglobulin G and M (HIgG and HIgM). The estimated binding constants from surface plasmon resonance (SPR) measurement were Ka = 9.4 x 10(6) M(-1) for HIgG and 5.4 x 10(6) M(-1) for HIgM, respectively. The binding site was not in the Fc region of immunoglobulin but in the Fab region. Large amounts of HIgG and HIgM bound to MEL-A SAMs were directly observed by atomic force microscopy.
authors.affiliation: Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Imura
authors.forename: Seya
authors.initials: S
authors.lastname: Ito
authors.forename: Reiko
authors.initials: R
authors.lastname: Azumi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yanagishita
authors.forename: Hideki
authors.initials: H
authors.lastname: Sakai
authors.forename: Masahiko
authors.initials: M
authors.lastname: Abe
authors.forename: Dai
authors.initials: D
authors.lastname: Kitamoto
mesh_terms: D002175:Candida / Q000737:chemistry*; D006017:Glycolipids / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000378:metabolism*; D007075:Immunoglobulin M / Q000737:chemistry / Q000378:metabolism*; D008024:Ligands; D018625:Microscopy, Atomic Force; D008954:Models, Biological; D015394:Molecular Structure; D011485:Protein Binding; D020349:Surface Plasmon Resonance; D013501:Surface-Active Agents / Q000737:chemistry / Q000378:metabolism
chemical_list: D006017:Glycolipids; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008024:Ligands; D013501:Surface-Active Agents; C439671:mannosylerythritol lipid