Relationship between MPA free fraction and free MPAG concentrations in heart transplant recipients based on simultaneous HPLC quantification of the target compounds in human plasma.
A simple and sensitive HPLC method for the simultaneous analysis of free MPA and free MPAG was developed. Separation was achieved on a X-Terra RP18 column with acetonitrile-40 mM orthophosphoric acid as eluents using a gradient elution mode over 35 min at a flow rate of 1.5 ml/min. The assay was linear in the range 0.005 mg/L (LOQ) to 5mg/L for free MPA and 0.05 mg/L (LOQ) to 200 mg/L for free MPAG. Isolation of free MPA and free MPAG was done by ultrafiltration and the ultrafiltrate was directly injected. A positive correlation between MPA free fractions and free MPAG concentrations was found. Likewise, free MPAG was related to total MPAG concentrations in the seven heart transplant patients.
Cussonneau
Xavier
X
Université de Lyon, Université Lyon 1, ISPB, Département de Pharmacie Clinique, de Pharmacocinétique et d'Evaluation du Médicament, INSERM ERI 22, Lyon F-69373, France.
Bolon-Larger
Magali
M
Prunet-Spano
Céline
C
Bastien
Olivier
O
Boulieu
Roselyne
R
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D020719:Glucuronides / Q000097:blood* / Q000493:pharmacokinetics; D016027:Heart Transplantation; D006801:Humans; D009173:Mycophenolic Acid / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D014462:Ultrafiltration
D020719:Glucuronides; C113145:mycophenolic acid glucuronide; D009173:Mycophenolic Acid