New immortalized cell lines of patients with small supernumerary marker chromosome: towards the establishment of a cell bank.
Sixteen newly established cell lines with small supernumerary marker chromosomes (sSMC) derived from chromosomes 1, 2, 4, 6, 7, 8, 14, 15, 16, 18, 19, 21, and 22 are reported. Two sSMC are neocentric and derived from 15q24.1-qter and 2q35-q36, respectively. Two further cases each present with two sSMC of different chromosomal origin. sSMC were characterized by multicolor fluorescence in situ hybridization for their chromosomal origin and genetic content. Moreover, uniparental disomy of the sister chromosomes of the sSMC was excluded in all nine cases studied for that reason. The 16 cases provide information to establish a refined genotype-phenotype correlation of sSMC and are available for future studies.
Tönnies
Holger
H
Institute of Human Genetics, Charité Campus Virchow, Berlin, Germany. i8lith@mti.uni-jena.de
Pietrzak
Joanna
J
Bocian
Ewa
E
MacDermont
Kay
K
Kuechler
Alma
A
Belitz
Britta
B
Trautmann
Udo
U
Schmidt
Angela
A
Schulze
Berndt
B
Rodríguez
Laura
L
Binkert
Franz
F
Yardin
Catharine
C
Kosyakova
Nadezda
N
Volleth
Marianne
M
Mkrtchyan
Hasmik
H
Schreyer
Isolde
I
von Eggeling
Ferdinand
F
Weise
Anja
A
Mrasek
Kristin
K
Liehr
Thomas
T
D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism / Q000821:virology; D018070:Biological Specimen Banks; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D002869:Chromosome Aberrations; D020223:Chromosome Painting / Q000379:methods*; D005260:Female; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D007621:Karyotyping; D008297:Male; D008957:Models, Genetic; D024182:Uniparental Disomy