title: Combination of hypoosmotic swelling/eosin Y test for sperm membrane integrity evaluation: correlations with other sperm parameters to predict ICSI cycles.
abstract: The objective of our study was to evaluate the accuracy of the combination of hypoosmotic swelling (HOS) and eosin Y (Ey) exclusion tests to predict the ICSI cycles' outcome and its correlations with other sperm parameters. The functional and structural integrity of sperm membrane was evaluated with the combined HOS/Ey test in 95 ICSI cycles and the results were correlated with other sperm parameters, including concentration, motility, strict morphology, and total motile sperm count. The combined HOS/Ey test was evaluated for the prediction of the ICSI cycles' outcome parameters including fertilization, cleavage, and pregnancy rates. The HOS/Ey test presented significant relationships with concentration, motility, and strict morphology (p < 0,0001) but it couldn't predict the fertilization, cleavage, and pregnancy outcomes of ICSI cycles. The combined HOS/Ey test has strong correlations with motility and strict morphology parameters of sperm samples but is not sufficiently sensitive to estimate the outcome of ICSI cycles.
authors.affiliation: Department of Obstetrics and Gynecology, Gulhane Military Medical Academy, Haydarpasa Training Hospital, Istanbul, Turkey. mcincik@yahoo.com
authors.forename: M
authors.initials: M
authors.lastname: Cincik
authors.forename: A R
authors.initials: AR
authors.lastname: Ergur
authors.forename: L
authors.initials: L
authors.lastname: Tutuncu
authors.forename: M
authors.initials: M
authors.lastname: Muhcu
authors.forename: M
authors.initials: M
authors.lastname: Kilic
authors.forename: B
authors.initials: B
authors.lastname: Balaban
authors.forename: B
authors.initials: B
authors.lastname: Urman
mesh_terms: D002462:Cell Membrane / Q000648:ultrastructure*; D006063:Chorionic Gonadotropin / Q000097:blood; D041322:Chromosomes, Human, Y / Q000502:physiology*; D004715:Endometriosis / Q000503:physiopathology; D005260:Female; D005306:Fertilization; D006801:Humans; D008297:Male; D009865:Oocytes / Q000166:cytology / Q000502:physiology; D010049:Ovarian Diseases / Q000503:physiopathology; D018579:Patient Selection; D011237:Predictive Value of Tests; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D016896:Treatment Outcome
chemical_list: D006063:Chorionic Gonadotropin