Confocal light absorption and scattering spectroscopic microscopy.
We have developed a novel optical method for observing submicrometer intracellular structures in living cells, which is called confocal light absorption and scattering spectroscopic (CLASS) microscopy. It combines confocal microscopy, a well-established high-resolution microscopic technique, with light-scattering spectroscopy. CLASS microscopy requires no exogenous labels and is capable of imaging and continuously monitoring individual viable cells, enabling the observation of cell and organelle functioning at scales of the order of 100 nm.
Fang
Hui
H
Biomedical Imaging and Spectroscopy Laboratory, Department of Medicine, Beth Israel Deaconess Medical Center, MA 02215, USA.
Qiu
Le
L
Vitkin
Edward
E
Zaman
Munir M
MM
Andersson
Charlotte
C
Salahuddin
Saira
S
Kimerer
Lauren M
LM
Cipolloni
Patsy B
PB
Modell
Mark D
MD
Turner
Bradley S
BS
Keates
Sarah E
SE
Bigio
Irving
I
Itzkan
Irving
I
Freedman
Steven D
SD
Bansil
Rama
R
Hanlon
Eugene B
EB
Perelman
Lev T
LT
D004867:Equipment Design; D019544:Equipment Failure Analysis; D005240:Feasibility Studies; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis / Q000295:instrumentation* / Q000379:methods; D041623:Tomography, Optical Coherence / Q000295:instrumentation* / Q000379:methods