Development of a multistage classifier for a monitoring system of cell activity based on imaging of chromosomal dynamics.
BACKGROUND
Cell-based assays utilizing digital image cytometry yield multivariate sets of information measuring the efficacy of medicines/chemicals. The use of a HeLa cell line that expresses a GFP-Histone-H1 fusion protein further enhances the performance of these systems, avoiding the use of dyes that may have detrimental influence on cells. Aside from the mitotic index, the distribution of the cell-cycle phases during mitosis can be used as measures of drug/treatment efficacy. Quantification of these parameters, however, requires skill and is time consuming. The purpose of this research was therefore to create a classifier to be incorporated into a system that can automatically identify the cell-cycle phases in a given image.


METHODS
Features based on the shape and texture of the chromosomal regions in images of live HeLa cells were measured and analyzed. Linear discriminant functions were calculated for the eight cell-cycle phases: interphase, prophase, prometaphase, metaphase, early anaphase, anaphase, telophase and cytokinesis.


RESULTS
The multistage linear discriminant classifier developed had an average classification efficiency of 87.30%.


CONCLUSIONS
We demonstrated the possibility of creating a classifier to discriminate between cell-cycle phases using shape and texture features of chromosomal regions. The classifier can be fused to an algorithm for image segmentation, forming a system to automatically and rapidly measure the aforementioned parameters. The results can then be collated to constitute an assay assessing the effects of a drug or treatment on mammalian cells.
Gambe
Arni E
AE
Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan.
Ono
Rika Maniwa
RM
Matsunaga
Sachihiro
S
Kutsuna
Natsumaro
N
Higaki
Takumi
T
Higashi
Tsunehito
T
Hasezawa
Seiichiro
S
Uchiyama
Susumu
S
Fukui
Kiichi
K
D002453:Cell Cycle / Q000502:physiology*; D002877:Chromosomes, Human / Q000378:metabolism*; D016002:Discriminant Analysis; D049452:Green Fluorescent Proteins; D006367:HeLa Cells; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D015999:Multivariate Analysis; D015203:Reproducibility of Results
D049452:Green Fluorescent Proteins