[Chemical constituents of the non-alkaloid fraction of Uncaria macrophylla].
OBJECTIVE
To analyze the chemical constituents of the non-alkaloid fraction of Uncaria macrophylla.


METHODS
The constituents were separated and purified by silica gel chromatography and recrystalization, and their structures were identified by infrared, mass and nuclear magnetic resonance spectrometry.


RESULTS
Six compounds were isolated from the non-alkaloid fraction of Uncaria macrophylla, whose structures were identified as beta-sitosterol, beta-daucosterol, ursolic acid, alpha-amyrin acetate, 3beta, 6beta, 23-trihydroxyurs-12-en-28-oic acid, 3beta-hydroxyurs-12-en-27, and 3beta-hydroxyurs-12-en-27, 28-dioic acid, respectively.


CONCLUSIONS
Ursolic acid is the main component in the non-alkaloid fraction of Uncaria macrophylla. Alpha-amyrin acetate and 3beta-hydroxyurs-12-en-27, 28-dioic acid are isolated and characterized for the first time from this plant.
Wu
Jun-Yan
JY
Second Hospital of Sun yet-sen University, Guangzhou 510120, China. junewu@tom.com
Li
Guo-Cheng
GC
Wang
Ding-Yong
DY
D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000302:isolation & purification; D014315:Triterpenes / Q000302:isolation & purification*; D032070:Uncaria / Q000737:chemistry*; D000097245:Ursolic Acid
D009828:Oleanolic Acid; D014315:Triterpenes; D000097245:Ursolic Acid; C528818:3beta,6beta,23-trihydroxyurs-12-en-28-oic acid; C528817:3beta-hydroxyurs-12-en-27, 28-dioic acid; C000265:beta-amyrin acetate