An in vitro model system used to study adherence and invasion of Francisella tularensis live vaccine strain in nonphagocytic cells.
In observing Francisella tularensis interactions with nonphagocytic cell lines in vitro, we noted significant adherence, invasion, and intracellular growth of the bacteria within these cells. F. tularensis live vaccine strain invasion of nonprofessional phagocytic cells is inhibited by cytochalasin D and nocodazole, suggesting that both the actin and microtubule cytoskeletons are important for invasion.
Lindemann
Stephen R
SR
Department of Microbiology, University of Iowa School of Medicine, 51 Newton Road, Iowa City, IA 52242-1109, USA.
McLendon
Molly K
MK
Apicella
Michael A
MA
Jones
Bradley D
BD
D001422:Bacterial Adhesion / Q000502:physiology*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D002460:Cell Line / Q000382:microbiology; D015638:Cytochalasin D / Q000494:pharmacology; D042783:Endothelial Cells / Q000378:metabolism / Q000382:microbiology*; D005604:Francisella tularensis / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D008954:Models, Biological; D010587:Phagocytosis; D014406:Tularemia / Q000517:prevention & control
D001428:Bacterial Vaccines; D015638:Cytochalasin D