[Promotion of the activity of Akt in CD8+ T cells by the crosslink of 3H3, an agonist antibody of 4-1BB].
OBJECTIVE
To investigate whether 4-1BB could promote the proliferation and survival of CD8(+) T cells proliferation, and the activity of protein kinase B (Akt).


METHODS
Highly purified CD8(+) T cells were separated and stimulated by CD3 antibody. The crosslink of 3H3 and the proliferation and antiapoptosis of CD8(+) T cells were examined.


RESULTS
The crosslink of 3H3 promoted the proliferation and survival of CD8(+) T cells. 4-1BB induced by 3H3 promoted the phosphorylated level and kinase activity of Akt.


CONCLUSIONS
Akt pathway might participate in 4-1BB which promoted the proliferation and survival of CD8(+) T cells induced by 3H3.
Yang
Zhao
Z
Institute of Immunology, Third Military Medical University, Chongqing, China. yangzhao5140@sohu.com
Fu
Xiao-Lan
XL
Shang
Xiao-Yun
XY
Niu
Wei
W
Wu
Yu-Zhang
YZ
Wang
Li
L
D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology* / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D010766:Phosphorylation / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D053261:Tumor Necrosis Factor Receptor Superfamily, Member 9 / Q000819:agonists* / Q000276:immunology*
D000906:Antibodies; D053261:Tumor Necrosis Factor Receptor Superfamily, Member 9; D051057:Proto-Oncogene Proteins c-akt