title: Oral administration of a heat-killed cell preparation of Enterococcus faecalis strain EC-12 stimulates the gene expression of Na(+)-K(+)-2Cl- co-transporter 1 in rat ileal epithelial cells.
abstract: The effect of a heat-killed cell preparation of Enterococcus faecalis strain EC-12 (EC-12) on the gene expression of Na(+)-K(+)-2Cl(-) co-transporter 1 (NKCC1) in intestinal epithelial cells was evaluated by using rats. The NKCC1 gene in ileal epithelial cells was significantly up-regulated by the oral administration of EC-12. The results of this study suggest in vivo that EC-12 had the potential to stimulate intestinal NKCC1 expression.
authors.affiliation: Laboratory of Animal Science, Kyoto Prefectural University, Shimogamo, Kyoto, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Inoue
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Tsukahara
authors.forename: Mamiko
authors.initials: M
authors.lastname: Noda
authors.forename: Wakoto
authors.initials: W
authors.lastname: Bukawa
authors.forename: Noritaka
authors.initials: N
authors.lastname: Matsubara
authors.forename: Kazunari
authors.initials: K
authors.lastname: Ushida
mesh_terms: D000284:Administration, Oral; D000818:Animals; D013293:Enterococcus faecalis; D004847:Epithelial Cells / Q000378:metabolism*; D006358:Hot Temperature; D007082:Ileum / Q000378:metabolism*; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D028021:Sodium-Potassium-Chloride Symporters / Q000096:biosynthesis*; D064506:Solute Carrier Family 12, Member 2
chemical_list: C578879:Slc12a2 protein, rat; D028021:Sodium-Potassium-Chloride Symporters; D064506:Solute Carrier Family 12, Member 2