Actin-binding proteins coronin-1a and IBA-1 are effective microglial markers for immunohistochemistry.
This study identifies the actin-binding protein, coronin-1a, as a novel and effective immunohistochemical marker for microglia in both cell cultures and in formaldehyde-fixed, paraffin-embedded tissue. Antibodies to coronin-1a effectively immunostained microglia in human, monkey, horse, rat, and mouse tissues, even in tissues stored for long periods of time. The identity of coronin-1a-immunoreactive cells as microglia was confirmed using double immunolabeling with cell type-specific markers as well as by morphological features and the distribution of immunoreactive cells. These properties are shared by another actin-binding protein, IBA-1. Unlike IBA-1, coronin-1a immunoreactivity was also detected in lymphocytes and certain other hematopoietic cells. The results indicate that both coronin-1a and IBA-1 are robust markers for microglia that can be used in routinely processed tissue of humans and animals. Because both coronin-1a and IBA-1 are actin-binding proteins that play a role in rearrangement of the membrane cytoskeleton, it suggests that these proteins are critical to dynamic properties of microglia.
Ahmed
Zeshan
Z
Department of Neuroscience, Mayo Clinic College of Medicine, Jacksonville, Florida, USA.
Shaw
Gerry
G
Sharma
Ved P
VP
Yang
Cui
C
McGowan
Eileen
E
Dickson
Dennis W
DW
D000199:Actins / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D018920:Coculture Techniques; D004285:Dogs; D018922:HL-60 Cells; D006736:Horses; D006801:Humans; D007150:Immunohistochemistry; D008253:Macaca mulatta; D051379:Mice; D008822:Mice, Transgenic; D008840:Microfilament Proteins / Q000378:metabolism*; D017628:Microglia / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D051381:Rats; D013045:Species Specificity
D000199:Actins; D015415:Biomarkers; D002135:Calcium-Binding Proteins; D008840:Microfilament Proteins; C072061:coronin proteins