title: Excentral cleavage of beta-carotene in vivo in a healthy man.
abstract: BACKGROUND
  Excentral cleavage of beta-carotene to retinoids and apocarotenoids occurs in vitro and in animal models. Whether it occurs in humans is unclear.
  
  
  OBJECTIVE
  We tested the hypothesis of whether humans can cleave beta-carotene excentrally.
  
  
  METHODS
  A healthy man was given an oral dose of all-trans [10,10',11,11'-(14)C]-beta-carotene (1.01 nmol; 100 nCi). Its fate and that of its metabolites were measured in serial plasma samples. Its fate in feces and urine was also measured over time. Selected plasma samples were spiked with reference standards of retinol, beta-apo-12'-carotenal, beta-apo-8'-carotenal, 13-cis-retinoic acid, all-trans-retinoic acid, beta-carotene-5,6-epoxide, all-trans-beta-carotene, and retinyl palmitate and subjected to reverse-phase HPLC fractionation. The plasma, plasma fractions, urine, and feces were measured for (14)C with the use of accelerator mass spectrometry.
  
  
  RESULTS
  Sixty-five percent of administered (14)C was absorbed, and 15.7% was eliminated in urine during the first 21 d after dosing. (14)C-beta-carotene and (14)C-retinyl palmitate appeared in plasma 0.25 d after the dose. (14)C-beta-carotene and (14)C-retinol both appeared at 0.5 d only. On day 3 after the dose, 2 large (14)C peaks appeared in plasma: one matched the retention time of beta-apo-8'-carotenal, and the other did not match any of the reference standards used. The delayed appearance of (14)C-beta-apo-8'-carotenal in plasma suggests that the excentral cleavage occurred after the (14)C-beta-apo-8'-carotene was absorbed into the body.
  
  
  CONCLUSIONS
  These data suggest that excentral cleavage of ingested beta-carotene occurs in vivo in humans. Confirmation of that possibility and further study to identify and characterize additional metabolites are needed.
authors.affiliation: Department of Nutrition, University of California, Davis, CA 95616-8669, USA.
authors.forename: Charlene C
authors.initials: CC
authors.lastname: Ho
authors.forename: Fabiana F
authors.initials: FF
authors.lastname: de Moura
authors.forename: Seung-Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Clifford
mesh_terms: D000328:Adult; D015992:Body Mass Index; D002250:Carbon Radioisotopes; D002851:Chromatography, High Pressure Liquid; D004032:Diet; D005243:Feces / Q000737:chemistry; D006801:Humans; D007275:Injections, Intravenous; D008297:Male; D011865:Radioisotope Dilution Technique; D019207:beta Carotene / Q000008:administration & dosage* / Q000097:blood* / Q000652:urine
chemical_list: D002250:Carbon Radioisotopes; D019207:beta Carotene