title: Transcriptome analysis of gills from the white shrimp Litopenaeus vannamei infected with White Spot Syndrome Virus.
abstract: We report the analysis of 872 cDNA clones from a WSSV-infected white shrimp Litopenaeus vannamei gill cDNA library. Comparison against the GenBank protein and nucleotide sequences identified 87% (E < or = 10(-2)) as previously known genes, while 13% are novel sequences. The 601 ESTs (87%) represent transcripts of 276 genes. These genes were categorized into 12 groups according to their functions. The more abundant categories were (1) ribosomal proteins (21%), (2) WSSV transcripts and sequences without homology to proteins deposited in the non-redundant database (15%), (3) hypothetical proteins (12%) which include genes never described in shrimp and (4) metabolism related proteins (9%). We also found genes involved in stress and immune response; and only one involved in ion transport. Full-length sequences of keratinocyte associated protein 2 (KCP2), selenoprotein M (SelM), chicadae, prohibitin and oncoprotein nm23 are reported. Their mRNAs steady state levels in addition to ferritin, changed at different times post-WSSV infection as estimated by RT-PCR. These results suggest that WSSV alters gene expression in gills and has led to the identification of novel white shrimp specific genes.
authors.affiliation: Aquatic Molecular Biology, Centro de Investigación en Alimentación y Desarrollo, Hermosillo, Sonora 83000, México.
authors.forename: Alejandra
authors.initials: A
authors.lastname: Clavero-Salas
authors.forename: Rogerio R
authors.initials: RR
authors.lastname: Sotelo-Mundo
authors.forename: Teresa
authors.initials: T
authors.lastname: Gollas-Galván
authors.forename: Jorge
authors.initials: J
authors.lastname: Hernández-López
authors.forename: Alma Beatriz
authors.initials: AB
authors.lastname: Peregrino-Uriarte
authors.forename: Adriana
authors.initials: A
authors.lastname: Muhlia-Almazán
authors.forename: Gloria
authors.initials: G
authors.lastname: Yepiz-Plascencia
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004266:DNA Virus Infections / Q000235:genetics* / Q000276:immunology / Q000821:virology; D005880:Gills / Q000276:immunology / Q000821:virology; D008969:Molecular Sequence Data; D015513:Oncogene Proteins / Q000737:chemistry / Q000235:genetics; D033561:Penaeidae / Q000235:genetics* / Q000276:immunology / Q000821:virology; D051304:Profilins / Q000737:chemistry / Q000235:genetics; D000091402:Prohibitins; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051140:Selenoproteins / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D014158:Transcription, Genetic; D046848:White spot syndrome virus 1 / Q000276:immunology*
chemical_list: D015513:Oncogene Proteins; D051304:Profilins; D000091402:Prohibitins; D012367:RNA, Viral; D012097:Repressor Proteins; D051140:Selenoproteins