Effects of dipeptides having a C-terminal lysine on the cholesterol 7alpha-hydroxylase mRNA level in HepG2 cells.
Inducing expression of the cholesterol-catabolizing enzyme cholesterol 7alpha-hydroxylase (CYP7A1) in the liver can be an effective strategy in preventing hypercholesterolemia and atherosclerosis. We used HepG2 cells to investigate the effects of 1 mM dipeptides having a C-terminal lysine group on the CYP7A1 mRNA level. We found that the dipeptides Asp-Lys, Glu-Lys, and Trp-Lys significantly increased the CYP7A1 mRNA level.
Morikawa
Kensei
K
Department of Applied Life Science, Faculty of Applied Biological Sciences, Gifu University, Yanagido, Gifu, Japan.
Ishikawa
Kumiko
K
Kanamaru
Yoshihiro
Y
Hori
Goro
G
Nagaoka
Satoshi
S
D000596:Amino Acids / Q000494:pharmacology*; D002460:Cell Line; D045744:Cell Line, Tumor; D002790:Cholesterol 7-alpha-Hydroxylase / Q000378:metabolism*; D004151:Dipeptides / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006801:Humans; D008239:Lysine / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism*
D000596:Amino Acids; D004151:Dipeptides; D012333:RNA, Messenger; D002790:Cholesterol 7-alpha-Hydroxylase; D008239:Lysine