title: The Drosophila SET domain encoding gene dEset is essential for proper development.
abstract: We have identified dEset, the fly homolog of human SETDB1 and mouse ESET histone lysine methyltransferases (HKMTases) that methylates the lysine 9 residue of histone 3 (H3-K9) and negatively regulates transcription of target genes. By using spatio-temporal RNA interference we show that dEset is required at several stages of development coinciding with ecdysone pulses, possibly as a repressor of transcription of target genes. Several interacting partners, for example USP, spire, and cut up were identified in a yeast two-hybrid screen. The spatio-temporal expression profiles of dEset and its potential partners suggest that they may act together or even in a larger complex.
authors.affiliation: Institute of Molecular Biosciences, University of Oslo, P.O.B. 1041 Blindern, NO-0316 Oslo, Norway.
authors.forename: Marianne
authors.initials: M
authors.lastname: Stabell
authors.forename: Mona
authors.initials: M
authors.lastname: Bjørkmo
authors.forename: Reidunn B
authors.initials: RB
authors.lastname: Aalen
authors.forename: Andrew
authors.initials: A
authors.lastname: Lambertsson
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D019521:Body Patterning / Q000235:genetics; D004330:Drosophila / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D029721:Drosophila Proteins / Q000235:genetics / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D011495:Histone-Lysine N-Methyltransferase / Q000235:genetics / Q000502:physiology*; D008297:Male; D051379:Mice; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010641:Phenotype; D011496:Protein Methyltransferases / Q000235:genetics; D017434:Protein Structure, Tertiary; D034622:RNA Interference; D020798:Two-Hybrid System Techniques
chemical_list: D029721:Drosophila Proteins; D011496:Protein Methyltransferases; D011495:Histone-Lysine N-Methyltransferase; C516239:SETDB1 protein, mouse; C519551:egg protein, Drosophila