Human antibody and antigen response to IncA antibody of Chlamydia trachomatis.
The high prevalence of C. trachomatis worldwide has underscored the importance of identifying specific immunogenic antigens in facilitating diagnosis as well as vaccine development. The aim of this study is to evaluate IncA antibody and antigen production in natural human infections. Our temporal expression study showed that IncA transcription and protein expression could be detected as early as 4 hours after the start of infection. Antibody responses could be detected in urine and genital swab samples from C. trachomatis-positive patients. It is especially interesting to note that the IncA antigen could be detected in urine. In conclusion, we have identified IncA as an important antigen in human. The potential applicability of the IncA antibody or antigen in the diagnosis as well as to vaccine development for C. trachomatis is also discussed.
Tsai
P Y
PY
Mycotic Diseases Laboratory, Research and Diagnostics Center, Centers for Disease Control, Taipei, Taiwan.
Hsu
M C
MC
Huang
C T
CT
Li
S Y
SY
D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000652:urine; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology* / Q000652:urine; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology; D015153:Blotting, Western; D002690:Chlamydia Infections / Q000276:immunology / Q000382:microbiology / Q000652:urine; D002692:Chlamydia trachomatis / Q000276:immunology*; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006367:HeLa Cells; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology*; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; C460868:IncA protein, Chlamydia trachomatis; D008565:Membrane Proteins