Isolation of transposon insertions.
Transposon insertions in and near a gene of interest facilitate the genetic characterization of a gene in vivo. This chapter is dedicated to describing the isolation of mini-Tn10 insertions in any desired nonessential gene in Salmonella enterica, as well as the isolation of mini-Tn10 insertions near particular genes. The protocols describe use of a tetracycline-resistant Tn10 derivative, but similar approaches can be used for derivatives resistant to other antibiotics. In addition, these approaches are directly applicable to other bacteria that have generalized transducing phages.
Maloy
Stanley R
SR
Department of Biology, Center for Microbial Sciences, San Diego State University, San Diego, CA, USA.
D004251:DNA Transposable Elements; D005798:Genes, Bacterial; D016254:Mutagenesis, Insertional; D019779:Salmonella enterica / Q000235:genetics; D014161:Transduction, Genetic
D004251:DNA Transposable Elements