Fetal calf serum-free culture of Chinese hamster ovary cells employing fish serum.
The effects of fish serum on cell growth and human granulocyte-macrophage colony-stimulating factor (hGM-CSF) production in an adhesion culture of Chinese hamster ovary (CHO) cells DR1000L4N were investigated and compared with those of fetal calf serum (FCS). Although fish serum did not stimulate the initial adhesion of CHO cells to culture dishes, it prompted cell growth after cell adhesion with FCS for 24 h. The cell density in the fish serum medium reached 75% that in the FCS medium. Fish serum promoted cell adhesion to and cell growth on collagen-coated dishes. The cell-specific production rate of hGM-CSF in the fish serum medium on collagen-coated dishes was almost the same as that in the FCS medium.
Fujiwara
M
M
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, North 13, West 8, Kita-ku, Sapporo, 060-8628, Japan.
Tsukada
R
R
Tsujinaga
Y
Y
Takagi
M
M
D000818:Animals; D016466:CHO Cells; D002417:Cattle; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D006224:Cricetinae; D003412:Cricetulus; D016895:Culture Media, Serum-Free; D005399:Fishes / Q000097:blood*; D044967:Serum
D016895:Culture Media, Serum-Free