Evaluation of the biocompatibility of acellular porcine dermis.
The biocompatibility of an acellular porcine dermis was evaluated by in vitro methods. Endothelia cells (ECV-304) and fibroblasts (NIH-3T3) were seeded on the dermis and cultured for 1 week to assess the cell viability in the skin grafts. Results by morphological assessment and methylthiazolyl tetrazolium assay (MTT assay) indicated good biocompatibility of acellular porcine dermis, which allowed adhesion and proliferation of examined cell types. Flow chamber technique was used to evaluate the adhesive force between cells and biomaterials. There was no significant difference in cells retention ratio between control cells and experimental cells after sheared 24h. The determination of integrin alpha5 expression proved that the acellular porcine dermis did not influence the expression of integrin alpha5 in cells. The results suggested that the dermal equivalent made from pigskin is a promising material for burn treatment.
Tang
Li-Ling
LL
Key Laboratory of Biomechanics and Tissue Engineering under the Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, PR China. lilingtang@yahoo.com.cn
Liu
Huan
H
Wang
Yuan-Liang
YL
Xian
Cheng-Yu
CY
Su
Ai-Hua
AH
D000818:Animals; D001672:Biocompatible Materials; D049109:Cell Proliferation; D020405:Dermis / Q000166:cytology; D013552:Swine
D001672:Biocompatible Materials