title: The determination of potassium concentration in vitreous humor by low pressure ion chromatography and its application in the estimation of postmortem interval.
abstract: An analytical method was developed for the determination of potassium in vitreous humor by low pressure ion chromatography (LPIC). Experimental conditions for LPIC analysis were optimized. High sensitivity and selectivity were obtained using this method. The LOD and LOQ were 1 and 2 mmol l(-1), respectively. The linearity was demonstrated from 2 to 20 mmol l(-1). The intra- and inter-day precision (CV) based on three concentrations was less than 5.0%. It was a simple and fast method to measure potassium and was suitable for evaluating the postmortem interval (PMI) in relatively well-preserved bodies. Sixty-two samples from medical-legal autopsies with known PMI were analyzed. A linear correlation equation for potassium concentration in the vitreous humor and PMI was established: [K(+)]=0.1702PMI+5.5678, r=0.8692.
authors.affiliation: West China Second University Hospital, Sichuan University, Chengdu 610041, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Gengqian
authors.initials: G
authors.lastname: Zhang
authors.forename: Xinshen
authors.initials: X
authors.lastname: Zhang
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000328:Adult; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D011180:Postmortem Changes; D011188:Potassium / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014822:Vitreous Body / Q000737:chemistry*
chemical_list: D011188:Potassium