title: Creating recombination-activated genes and sequence-defined mutant libraries using transposons.
abstract: The properties of a collection of transposon Tn5 derivatives that generate reporter gene fusions and internal protein tags are summarized. Procedures utilizing several of the transposons for generating genes activated by Cre-loxP recombination and for creating large sequence-defined mutant libraries are described in detail.
authors.affiliation: Department of Genome Sciences, University of Washington, Seattle, WA, USA.
authors.forename: Larry
authors.initials: L
authors.lastname: Gallagher
authors.forename: Cheri
authors.initials: C
authors.lastname: Turner
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Ramage
authors.forename: Colin
authors.initials: C
authors.lastname: Manoil
mesh_terms: D001483:Base Sequence; D004251:DNA Transposable Elements; D015723:Gene Library; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D011995:Recombination, Genetic
chemical_list: D004251:DNA Transposable Elements