[Lipopolysaccharides and lipoteichoic acids stimulate rat mast cells to cysteinyl leukotriene synthesis].
We have investigated the ability of lipopolysaccharides (LPS) and lipoteichoic acids (LTA) to induce rat peritoneal mast cells to degranulation and histamine release, and to cysteinyl leukotriene (LT) generation. We have stated that LPS Salmonella Enteritidis, LPS Escherichia coli O111:B4 and LPS E. coli O55:B5 did not activate rat mast cells to degranulation and histamine release. However, LPSs induced LT synthesis and secretion; the strongest stimulant to generation of LT was LPS E. coli O55:B5 (concentration of LT in supernatant was 830.5 +/-15.2 pg/ml). We have also observed that LTA Staphylococcus aureus and LTA Bacillus subtilis stimulated rat mast cells to degranulation and histamine secretion, even though the percentage of the releases histamine was relatively low (10.0 +/- 1.4 and 10.4 +/- 5.4 at antigen concentration, respectively). At the same time, LTA of both of the bacterial species strongly activate LT generation by mast cells (concentrations of LT in supernatants were 777.9 +/- 11.2 pg/ml and 734.0 +/- 38.3 pg/ml, respectively, at the antigen concentration 50 ng/ml). Our results have shown that LPS oraz LTA activate rat mast cells to secretion of proinflammatory mediators.
Brzezińska-Błaszczyk
Ewa
E
Zakład Immunologii Doświadczalnej Uniwersytetu Medycznego w Lodzi. ewab@csk.umed.lodz.pl
Rdzany
Rafal S
RS
D000818:Animals; D003545:Cysteine / Q000096:biosynthesis* / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology; D006636:Histamine Release; D007155:Immunologic Factors / Q000096:biosynthesis; D018836:Inflammation Mediators / Q000378:metabolism; D015289:Leukotrienes / Q000096:biosynthesis* / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology*; D008407:Mast Cells / Q000276:immunology* / Q000378:metabolism*; D051379:Mice; D010537:Peritoneum / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar; D013210:Staphylococcus / Q000276:immunology; D013682:Teichoic Acids / Q000276:immunology*
D007155:Immunologic Factors; D018836:Inflammation Mediators; D015289:Leukotrienes; D008070:Lipopolysaccharides; D013682:Teichoic Acids; C112381:cysteinyl-leukotriene; C009900:lipoteichoic acid; D003545:Cysteine