Ostreolysin affects rat aorta ring tension and endothelial cell viability in vitro.
The effects of ostreolysin, a cardiotoxic cytolysin produced by the edible oyster mushroom (Pleurotus ostreatus), were studied on tension development in isolated rat aortic ring. Its cytotoxic effects on human umbilical vein endothelial cells and Chinese hamster lung fibroblasts were also studied. Ostreolysin induced a concentration-dependent increase in aortic ring tension in the range from 5 to 30 microg/ml, and was cytotoxic to both cell lines. These effects could contribute significantly to its previously observed cardiotoxicity.
Rebolj
Katja
K
Department of Biology, Biotechnical Faculty, University of Ljubljana, Vecna pot 111, 1000 Ljubljana, Slovenia.
Batista
Urska
U
Sepcić
Kristina
K
Cestnik
Vojteh
V
Macek
Peter
P
Frangez
Robert
R
D000818:Animals; D001011:Aorta / Q000187:drug effects* / Q000502:physiology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D006224:Cricetinae; D003412:Cricetulus; D042783:Endothelial Cells / Q000187:drug effects*; D005656:Fungal Proteins / Q000032:analysis / Q000633:toxicity; D006460:Hemolysin Proteins / Q000032:analysis / Q000633:toxicity*; D006801:Humans; D009129:Muscle Tonus / Q000187:drug effects*; D020076:Pleurotus / Q000737:chemistry*; D051381:Rats; D013778:Tetrazolium Salts; D013844:Thiazoles
D005656:Fungal Proteins; D006460:Hemolysin Proteins; D013778:Tetrazolium Salts; D013844:Thiazoles; C461280:ostreolysin; C022616:thiazolyl blue