Friend of GATA-1-independent transcriptional repression: a novel mode of GATA-1 function.
The GATA-1-interacting protein Friend Of GATA-1 (FOG-1) is essential for the proper transcriptional activation and repression of numerous GATA-1 target genes. Although FOG-1-independent activation by GATA-1 has been described, all known examples of GATA-1-mediated repression are FOG-1 dependent. In the GATA-1-null G1E cell line, estrogen receptor ligand binding domain (ER) chimeras of either wild-type GATA-1 or a FOG-1-binding defective mutant of GATA-1 repressed several genes similarly upon activation with beta-estradiol. Repression also occurred in a FOG-1-null cell line expressing ER-GATA-1 and during ex vivo erythropoiesis. At the Lyl1 and Rgs18 loci, we found highly restricted occupancy by GATA-1 and GATA-2, indicating that these genes are direct targets of GATA factor regulation. The identification of genes repressed by GATA-1 independent of FOG-1 defines a novel mode of GATA-1-mediated transcriptional regulation.
Johnson
Kirby D
KD
Department of Pharmacology, University of Wisconsin School of Medicine, Madison, WI, USA.
Boyer
Meghan E
ME
Kang
Jeong-Ah
JA
Wickrema
Amittha
A
Cantor
Alan B
AB
Bresnick
Emery H
EH
D000818:Animals; D001665:Binding Sites; D050982:GATA1 Transcription Factor / Q000235:genetics / Q000502:physiology*; D005786:Gene Expression Regulation; D051379:Mice; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics*; D011960:Receptors, Estrogen / Q000235:genetics; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
D050982:GATA1 Transcription Factor; C494689:Gata1 protein, mouse; D009687:Nuclear Proteins; D011960:Receptors, Estrogen; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; C107310:Zfpm1 protein, mouse