Chitosan-alginate microcapsules for oral delivery of egg yolk immunoglobulin (IgY).
Chitosan-alginate microcapsules were evaluated as a method of oral delivery of IgY antibodies. Physical characteristics, encapsulation efficiency (EE%), the loading capacity for IgY (IgY loading percentage, %, w/w of microcapsules), gastro-resistance, and release characteristics of these microcapsules in vitro under varying pH were investigated. Optimum physical factors were established for preparation of homogeneous, spherical, and smooth microcapsules. IgY loading% was not significantly altered by pH of the encapsulation medium. Encapsulation efficiency was highest (73.93%) at a pH of 3.5, above which EE% decreased significantly (p < 0.05). IgY was released from microcapsules upon exposure to simulated intestinal fluid (SIF, pH 6.8), and decreasing pH increased significantly IgY release (p < 0.05). The stability of IgY in simulated gastric fluid (SGF, pH 1.2) was greatly improved by encapsulation in chitosan-alginate microcapsules, and the residual activity was not affected by pH of the encapsulation medium. Moreover, microencapsulated IgY was significantly resistant to pepsin hydrolysis. This approach may enable intact IgY to reach target microorganisms within the lower digestive tract.
Li
Xiao-Yu
XY
Department of Bioscience and Biotechnology, Dalian University of Technology, Dalian 116024, China.
Jin
Li-Ji
LJ
McAllister
Tim A
TA
Stanford
Kim
K
Xu
Jing-Yi
JY
Lu
Ya-Nan
YN
Zhen
Yu-Hong
YH
Sun
Yong-Xin
YX
Xu
Yong-Ping
YP
D000464:Alginates; D000818:Animals; D002214:Capsules; D002645:Chickens; D048271:Chitosan; D004337:Drug Carriers / Q000737:chemistry; D004355:Drug Stability; D004530:Egg Yolk / Q000276:immunology*; D005260:Female; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D007136:Immunoglobulins / Q000008:administration & dosage*
D000464:Alginates; D002214:Capsules; D004337:Drug Carriers; D006603:Hexuronic Acids; C016512:IgY; D007136:Immunoglobulins; D020723:Glucuronic Acid; D048271:Chitosan