Xylogalacturonan exists in cell walls from various tissues of Arabidopsis thaliana.
Evidence is presented for the presence of xylogalacturonan (XGA) in Arabidopsis thaliana. This evidence was obtained by extraction of pectin from the seeds, root, stem, young leaves and mature leaves of A. thaliana, followed by treatment of these pectin extracts with xylogalacturonan hydrolase (XGH). Upon enzymatic treatment, XGA oligosaccharides were primarily produced from pectin extracts obtained from the young and mature leaves and to a lesser extent from those originating from the stem of A. thaliana. The oligosaccharide GalA(3)Xyl was predominantly formed from these pectin extracts. No XGA oligosaccharides were detected in digests of pectin extracts from the seeds and roots. A low number of XGA oligosaccharides was obtained from pectins of A. thaliana. This indicates a uniform distribution of xylose in XGA from A. thaliana. The predominant production of GalA(3)Xyl, as well as the release of linear GalA oligosaccharides pointed to a lower degree of xylose substitution in XGA from A. thaliana than in XGA from apple and potato. The estimated amount of XGA accounted for approximately 2.5%, 7% and 6% (w/w) of the total carbohydrate in the pectin fraction of the stem, young leaves and mature leaves, respectively.
Zandleven
Joris
J
Laboratory of Food Chemistry, Department of Agrotechnology and Food Sciences, Wageningen University, P.O. Box 8129, 6700 EV Wageningen, The Netherlands.
Sørensen
Susanne Oxenbøll
SO
Harholt
Jesper
J
Beldman
Gerrit
G
Schols
Henk A
HA
Scheller
Henrik V
HV
Voragen
Alphons J
AJ
D017360:Arabidopsis / Q000737:chemistry*; D002473:Cell Wall / Q000737:chemistry*; D005591:Chemical Fractionation; D006603:Hexuronic Acids / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D006868:Hydrolysis; D010368:Pectins / Q000737:chemistry / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D012639:Seeds / Q000737:chemistry
D006603:Hexuronic Acids; D010936:Plant Extracts; C465879:xylogalacturonan; D010368:Pectins