title: Distinct subsets of microRNAs are expressed differentially in the human placentas of patients with preeclampsia.
abstract: OBJECTIVE
  Preeclampsia and small-for-gestational age (SGA) neonates have partially overlapping clinicopathologic features. MicroRNAs (miRNAs) are critical posttranscriptional regulators of gene expression. This study was performed to determine whether preeclampsia and SGA are associated with alterations in placental miRNA expression.
  
  
  METHODS
  Placentas were obtained from patients with (1) preeclampsia (n = 9); (2) SGA (n = 9); (3) preeclampsia + SGA (n = 9); and (4) a control group with spontaneous preterm labor and delivery (PTL; n = 9). The expression of 157 miRNAs was assessed by real-time quantitative reverse transcription-polymerase chain reaction.
  
  
  RESULTS
  Differential expression between preeclampsia and the control group (miR-210, miR-182) and between preeclampsia + SGA and the control group (miR-210, miR-182*, and others) was found. Gene Ontology analysis of the target genes revealed enrichment for specific biological process categories (antiapoptosis: miR-182; regulation of transcription: miR-210).
  
  
  CONCLUSIONS
  This study reports, for the first time, increased expression of specific placental miRNAs in preeclampsia with and without SGA. The findings also provide novel targets for further investigation of the pathophysiology of preeclampsia.
authors.affiliation: Perinatology Research Branch, National Institute of Child Health and Human Development/National Institutes of Health/Department of Health and Human Services, Bethesda, MD, USA.
authors.forename: Beth L
authors.initials: BL
authors.lastname: Pineles
authors.forename: Roberto
authors.initials: R
authors.lastname: Romero
authors.forename: Daniel
authors.initials: D
authors.lastname: Montenegro
authors.forename: Adi L
authors.initials: AL
authors.lastname: Tarca
authors.forename: Yu Mi
authors.initials: YM
authors.lastname: Han
authors.forename: Yeon Mee
authors.initials: YM
authors.lastname: Kim
authors.forename: Sorin
authors.initials: S
authors.lastname: Draghici
authors.forename: Jimmy
authors.initials: J
authors.lastname: Espinoza
authors.forename: Juan Pedro
authors.initials: JP
authors.lastname: Kusanovic
authors.forename: Pooja
authors.initials: P
authors.lastname: Mittal
authors.forename: Sonia S
authors.initials: SS
authors.lastname: Hassan
authors.forename: Chong Jai
authors.initials: CJ
authors.lastname: Kim
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D007236:Infant, Small for Gestational Age / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis*; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy
chemical_list: D035683:MicroRNAs