title: Monolithic poly[(trimethylsilyl-4-methylstyrene)-co- bis(4-vinylbenzyl)dimethylsilane] stationary phases for the fast separation of proteins and oligonucleotides.
abstract: In this paper the synthesis, optimisation and application of a silane based monolithic copolymer for the rapid separation of proteins and oligonucleotides is described. The monolith was prepared by thermal initiated in situ copolymerisation of trimethylsilyl-4-methylstyrene (TMSiMS) and bis(4-vinylbenzyl)dimethylsilane (BVBDMSi) in a silanised 200 microm I.D. fused silica column. Different ratios of monomer and crosslinker, as well as different ratios of micro- (toluene) and macro-porogen (2-propanol) were used for optimising the physical properties of the stationary phase regarding separation efficiency. The prepared monolithic stationary phases were characterised by measurement of permeability with different solvents, determination of pore size distribution by mercury intrusion porosimetry (MIP). Morphology was studied by scanning electron microscopy (SEM). Applying optimised conditions, a mixture comprised of five standard proteins ribunuclease A, cytochrome c, alpha-lactalbumine, myoglobine and ovalbumine was separated within 1 min by ion-pair reversed-phase liquid chromatography (IP-RPLC) obtaining half-height peak widths between 1.8 and 2.4 s. Baseline separation of oligonucleotides d(pT)(12-18) was achieved within 1.8 min obtaining half-height peak widths between 3.6 and 5.4 s. The results demonstrate the high potential of this stationary phase for fast separation of high-molecular weight biomolecules such as oligonucleotides and proteins.
authors.affiliation: Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens University, Innrain 52a, 6020 Innsbruck, Austria.
authors.forename: Thomas A E
authors.initials: TA
authors.lastname: Jakschitz
authors.forename: Christian W
authors.initials: CW
authors.lastname: Huck
authors.forename: Said
authors.initials: S
authors.lastname: Lubbad
authors.forename: Günther K
authors.initials: GK
authors.lastname: Bonn
mesh_terms: D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods; D007475:Ion Exchange Resins / Q000737:chemistry; D046208:Microchip Analytical Procedures / Q000379:methods; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D009841:Oligonucleotides / Q000032:analysis / Q000302:isolation & purification*; D010539:Permeability; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D011137:Polystyrenes / Q000138:chemical synthesis* / Q000737:chemistry; D016062:Porosity; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012821:Silanes / Q000138:chemical synthesis / Q000737:chemistry*; D013343:Styrenes / Q000737:chemistry; D013997:Time Factors; D014753:Vinyl Compounds / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D007475:Ion Exchange Resins; D009841:Oligonucleotides; D011108:Polymers; D011137:Polystyrenes; D011506:Proteins; D012821:Silanes; D013343:Styrenes; D014753:Vinyl Compounds; C521540:poly((trimethylsilyl-4-methylstyrene)-co- bis(4-vinylbenzyl)dimethylsilane)