Characterization of ITS2 rDNA of Anopheles philippinensis and Anopheles nivipes (Diptera: Culicidae) from north-east India.
Anopheles (Cellia) philippinensis Ludlow and Anopheles (Cellia) nivipes (Theobald) are two closely related, morphologically very similar, mosquito species in the Annularis group, which play a supportive role in malaria transmission in north-east India. We amplified and performed sequence analysis for the internal transcribed spacer 2 (ITS2) locus of ribosomal DNA (rDNA) gene of morphologically confirmed specimens of these two species from the states of Assam and Nagaland. An. philippinensis and An. nivipes shared 85.2% sequence similarity and no intra-species variation was found in the nucleotide sequences of the two species. Overall, sequence data of the ITS2 marker revealed that both these species from north-east India differed by as much as have been reported from specimens of eastern Thailand.
Prakash
Anil
A
Regional Medical Research Center, NE (Indian Council of Medical Research), Dibrugarh, Assam, India.
Walton
C
C
Bhattacharyya
D R
DR
Mohapatra
P K
PK
Mahanta
J
J
D000818:Animals; D000852:Anopheles / Q000235:genetics* / Q000469:parasitology; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D014644:Genetic Variation; D007194:India; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D021903:DNA, Ribosomal Spacer