title: Assay methods for antihepatotoxic activity using peroxide-induced cytotoxicity in primary cultured hepatocytes1.
abstract: Conditions were investigated to devise IN VITRO assay methods for antihepatotoxic activity using peroxide-induced damage in primary cultured rat liver cells. Utilizing linoleic acid peroxide, benzoyl peroxide, cumene hydroperoxide and adenosine diphosphate/Fe (3+), satisfactory assay procedures were established. Some natural products known to exert liver-protective effects IN VIVO were screened to reveal that glycyrrhetinic acid, glycyrrhizin, cynarin, silybin and desoxypodophyllotoxin showed similar antihepatotoxic activity in these four assay methods.
authors.affiliation: Pharmaceutical Institute, Tohoku University, Aoba-yama, Sendai, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kiso
authors.forename: O
authors.initials: O
authors.lastname: Kato
authors.forename: H
authors.initials: H
authors.lastname: Hikino