title: Characterization of ITS2 rDNA of Anopheles philippinensis and Anopheles nivipes (Diptera: Culicidae) from north-east India.
abstract: Anopheles (Cellia) philippinensis Ludlow and Anopheles (Cellia) nivipes (Theobald) are two closely related, morphologically very similar, mosquito species in the Annularis group, which play a supportive role in malaria transmission in north-east India. We amplified and performed sequence analysis for the internal transcribed spacer 2 (ITS2) locus of ribosomal DNA (rDNA) gene of morphologically confirmed specimens of these two species from the states of Assam and Nagaland. An. philippinensis and An. nivipes shared 85.2% sequence similarity and no intra-species variation was found in the nucleotide sequences of the two species. Overall, sequence data of the ITS2 marker revealed that both these species from north-east India differed by as much as have been reported from specimens of eastern Thailand.
authors.affiliation: Regional Medical Research Center, NE (Indian Council of Medical Research), Dibrugarh, Assam, India.
authors.forename: Anil
authors.initials: A
authors.lastname: Prakash
authors.forename: C
authors.initials: C
authors.lastname: Walton
authors.forename: D R
authors.initials: DR
authors.lastname: Bhattacharyya
authors.forename: P K
authors.initials: PK
authors.lastname: Mohapatra
authors.forename: J
authors.initials: J
authors.lastname: Mahanta
mesh_terms: D000818:Animals; D000852:Anopheles / Q000235:genetics* / Q000469:parasitology; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D014644:Genetic Variation; D007194:India; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D021903:DNA, Ribosomal Spacer