Levels of metalloproteinase-2 and -9 and tissue inhibitor of matrix metalloproteinase-1 in gingival crevicular fluid of patients with periodontitis, gingivitis, and healthy gingiva.
OBJECTIVE
To compare the levels of matrix metalloproteinase-2 and -9 (MMP-2 and MMP-9) and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) in patients with gingivitis and periodontitis and in healthy controls.


METHODS
Levels of MMP-2, MMP-9, and TIMP-1 were determined by ELISA in patients with healthy gingiva (n = 16), gingivitis (n = 18), and periodontitis (n = 25). The subjects with periodontitis were examined before and 1 month after basic periodontal therapy.


RESULTS
Slightly higher concentrations of MMP-9 were observed in patients with periodontitis but without statistical significance. MMP-2 was found to be slightly diminished in these patients. The lowest concentrations of MMP inhibitor (TIMP-1) were observed in patients with periodontitis, in whom the concentrations increased after periodontal treatment.


CONCLUSIONS
The reduction in TIMP-1 concentrations in disease situations suggests a breakdown of the balance between the amount of MMPs and their inhibitor.
Maeso
Gema
G
Department of Oral Medicine and Periodontology, School of Dentistry, University Compultense, Madrid, Spain.
Bravo
Manuel
M
Bascones
Antonio
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D005891:Gingivitis / Q000201:enzymology*; D006801:Humans; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000032:analysis / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000032:analysis / Q000378:metabolism*; D008875:Middle Aged; D010518:Periodontitis / Q000201:enzymology*; D018709:Statistics, Nonparametric; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000032:analysis / Q000378:metabolism*
D019715:Tissue Inhibitor of Metalloproteinase-1; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9