[Concentration of thyroid stimulating hormone and activity of N-acetyl-beta-D-hexosaminidase and its isoenzymes, in serum of patients with thyroid cancer].
Thyroid cancer consists 1% of all malignant neoplasms. It is not known interrelationship between concentration of TSH in blood serum and condition of thyroid cancer. Thyroid cancer is difficult for diagnosis and differentiation. Therefore it is necessary to search for biochemical markers helpful in diagnostics of thyroid cancer. Significant increase in activity of N-acetyl-beta-D-hexosaminidase and its isoenzymes A and B in serum of patients with neoplasms of kidney and pancreas suggest approporiateness of evaluation of HEX and its isoenzymes in diagnostics of thyroid cancer.


OBJECTIVE
of the study--evaluation of TSH concentration and activity of HEX and its isoenzymes A and B, in serum of patients with thyroid cancer.


METHODS
Blood was taken from 7 patients with thyroid cancer (6 men and 1 woman). Control consisted of 7 healthy men. In blood serum concentration of TSH was determined with immunoenzymatic method on analyzer Axsym of Abbott and expressed in microU/mL. The activity of HEX and its isoenzymes A and B was determined by method of Chatterjee et al., as modified by Zwierz et.al. Determination of HEX was performed on microplate reader ELX800 BIO-TEK. Activity of HEX, HEX A and B was expressed in pKat/mL, and specific activity in pKat/mg protein). Protein was determined by biuret method and results were expressed in mg/mL.


RESULTS
Concentration of HEX A activity in serum of thyroid cancer patients is significantly higherin comparison to healthymen (p = 0.0191). Also specific activity of HEX A in serum of thyroid cancer patients is significantly higher in comparison to healthy men (p = 0.0393).


CONCLUSIONS
1. Determination of TSH concentration in serum of thyroid cancer before the operation may confirm euthyreosis. 2. Determination of HEX A activity in serum may be helpful in diagnostics of thyroid cancer.
Zwierz
Piotr
P
Department of Pharmaceutical Biochemistry, Medical University of Białystok, Poland. kzwierz@amb.edu.pl
Szajda
Sławomir D
SD
Snarska
Jadwiga
J
Supronowicz
Zbigniew B
ZB
Zawadzki
Paweł
P
Zwierz
Krzysztof
K
Kamińsk
Fabian
F
D000328:Adult; D014408:Biomarkers, Tumor / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D054818:Hexosaminidase A; D006801:Humans; D007527:Isoenzymes / Q000097:blood; D008297:Male; D008875:Middle Aged; D044967:Serum / Q000201:enzymology*; D013964:Thyroid Neoplasms / Q000097:blood / Q000201:enzymology*; D013972:Thyrotropin / Q000097:blood*; D001619:beta-N-Acetylhexosaminidases / Q000097:blood*
D014408:Biomarkers, Tumor; D007527:Isoenzymes; D013972:Thyrotropin; D054818:Hexosaminidase A; D001619:beta-N-Acetylhexosaminidases