title: Cell adhesion and growth to Peptide-patterned supported lipid membranes.
abstract: Lipid vesicles displaying RGD peptide amphiphiles were fused with glass coverslips to control the ability of these surfaces to support cell adhesion and growth. Cell adhesion was prevented on phosphatidylcholine bilayers in the absence of RGD, whereas cells adhered and grew in the presence of accessible RGD amphiphiles. This specific interaction between cells and RGD peptides was further explored in a concentration-dependent fashion by creating surface composition arrays using microfluidics. For the range of concentrations studied adhesion and growth were favored by increased peptide concentration, but this concentration dependence was found to diminish in the higher concentration regions of the array. Developing peptide composition gradients in a membrane environment is demonstrated as an effective method to screen biological probes for cell adhesion and growth.
authors.affiliation: Department of Chemical Engineering and Materials Research Laboratory, University of California, Santa Barbara, California 93106, USA.
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Stroumpoulis
authors.forename: Haining
authors.initials: H
authors.lastname: Zhang
authors.forename: Leticia
authors.initials: L
authors.lastname: Rubalcava
authors.forename: Jill
authors.initials: J
authors.lastname: Gliem
authors.forename: Matthew
authors.initials: M
authors.lastname: Tirrell
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D005347:Fibroblasts / Q000166:cytology*; D008051:Lipid Bilayers; D051379:Mice; D044085:Microfluidics; D009842:Oligopeptides
chemical_list: D008051:Lipid Bilayers; D009842:Oligopeptides; C421251:RGD891