title: Improved luciferase tagging system for Listeria monocytogenes allows real-time monitoring in vivo and in vitro.
abstract: An improved system for luciferase tagging Listeria monocytogenes was developed by constructing a highly active, constitutive promoter. This construct gave 100-fold-higher activity in broth than any native promoter tested and allowed for imaging of lux-tagged L. monocytogenes in food products, during murine infections, and in tumor targeting studies.
authors.affiliation: Alimentary Pharmabiotic Centre and Department of Microbiology, University College Cork, Cork, Ireland.
authors.forename: Christian U
authors.initials: CU
authors.lastname: Riedel
authors.forename: Ian R
authors.initials: IR
authors.lastname: Monk
authors.forename: Pat G
authors.initials: PG
authors.lastname: Casey
authors.forename: David
authors.initials: D
authors.lastname: Morrissey
authors.forename: Gerald C
authors.initials: GC
authors.lastname: O'Sullivan
authors.forename: Mark
authors.initials: M
authors.lastname: Tangney
authors.forename: Colin
authors.initials: C
authors.lastname: Hill
authors.forename: Cormac G M
authors.initials: CG
authors.lastname: Gahan
mesh_terms: D000818:Animals; D001483:Base Sequence; D005513:Food Inspection / Q000379:methods*; D005516:Food Microbiology; D005822:Genetic Vectors / Q000235:genetics; D008089:Listeria monocytogenes / Q000378:metabolism*; D008156:Luciferases / Q000378:metabolism*; D049453:Luciferases, Bacterial; D051379:Mice; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D008156:Luciferases; D049453:Luciferases, Bacterial