Effect of dietary fish oil supplementation on cellular adhesion molecule expression and tissue myeloperoxidase activity in diabetic mice with sepsis.
This study investigated the effect of n-3 fatty acids on adhesion molecules and tissue myeloperoxidase (MPO) activity in diabetic mice with sepsis. Diabetes was induced by a streptozotocin injection. Mice with blood glucose levels exceeding 2000 mg/l were considered diabetic. Diabetic mice were assigned to two groups with a medium-fat (10 %, w/w) diet either provided by soyabean oil (SO, n 30) or fish oil (FO, n 30). n-3 fatty acids provided 4.3 % of the total energy and the n-3/n-6 fatty acid ratio was 1:2 in the FO diet. After feeding the respective diet for 3 weeks, all mice had sepsis induced by caecal ligation and puncture (CLP) and were killed at 0, 6 or 24 h after CLP, with ten mice at each time-point. The result showed that compared with the SO group, FO group had lower PGE2 and TNF-alpha levels in peritoneal lavage fluid after CLP. Lymphocyte CD11a/CD18 expressions were higher at 6 h, whereas the percentage was lower at 24 h in the SO group than in the FO group. Neutrophil CD11b/CD18 expressions were significantly higher in the SO group than in the FO group at 0 h. The FO group had lower organ MPO activities at various time-points after CLP when compared with those of the SO group. The present findings suggest that compared with the diabetic mice fed SO, a low-dose n-3 fatty acid supplementation may attenuate leucocyte adhesion and infiltration into tissues in diabetic mice complicated with sepsis.
Chiu
Wan-Chun
WC
Graduate Institute of Pharmacy, Taipei Medical University, 250 Wu-Hsing Street, Taipei, Taiwan, ROC.
Hou
Yu-Chen
YC
Yeh
Chiu-Li
CL
Hu
Ya-Mei
YM
Yeh
Sung-Ling
SL
D000818:Animals; D001202:Ascitic Fluid / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight; D040881:CD11a Antigen / Q000097:blood; D039481:CD11b Antigen / Q000097:blood; D018821:CD18 Antigens / Q000097:blood; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000097:blood*; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000378:metabolism*; D019587:Dietary Supplements; D015232:Dinoprostone / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology; D005395:Fish Oils / Q000494:pharmacology*; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009195:Peroxidase / Q000378:metabolism*; D018805:Sepsis / Q000150:complications / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D001786:Blood Glucose; D040881:CD11a Antigen; D039481:CD11b Antigen; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; D009195:Peroxidase; D015232:Dinoprostone