Immunoisolating pancreatic islets by encapsulation with selective withdrawal.
This manuscript reports the application of the selective-withdrawal coating technique to the microencapsulation of insulin-producing pancreatic islets within thin poly(ethylene glycol) coatings. These polymer coatings permit the islets to respond to changes in glucose concentration by producing insulin with a dose-response profile that is substantially similar to that of unencapsulated islets. Furthermore, the hydrogel capsules exclude the large molecules of the immune system. These results suggest that the microencapsulation technique-which combines droplet formation from a flow of two immiscible fluids with polymerization chemistries-has the characteristics required for the transplantation of islets for the treatment of Type I diabetes.
Wyman
Jason L
JL
The James Franck Institute and Department of Physics, The University of Chicago, Chicago, IL 60637, USA.
Kizilel
Seda
S
Skarbek
Ryan
R
Zhao
Xiangyang
X
Connors
Matthew
M
Dillmore
William S
WS
Murphy
William L
WL
Mrksich
Milan
M
Nagel
Sidney R
SR
Garfinkel
Marc R
MR
D000818:Animals; D002214:Capsules / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003922:Diabetes Mellitus, Type 1 / Q000628:therapy*; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D007107:Immune System; D007515:Islets of Langerhans / Q000276:immunology* / Q000378:metabolism*; D016381:Islets of Langerhans Transplantation / Q000295:instrumentation* / Q000379:methods*; D037102:Lectins / Q000737:chemistry; D018613:Microscopy, Confocal; D008970:Molecular Weight; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D013552:Swine
D002214:Capsules; D020099:Coated Materials, Biocompatible; D020100:Hydrogels; D037102:Lectins; D011108:Polymers; D011092:Polyethylene Glycols