title: Myeloid and lymphoid dendritic cells in the peritoneal fluid of women with ovarian cancer.
abstract: OBJECTIVE
  The aim of the study was to estimate the myeloid and lymphoid subpopulation of dendritic cells (DCs) in the peritoneal fluid (PF) of women with ovarian tumors.
  
  
  METHODS
  We studied 34 patients with serous cystadenocarcinoma and 29 women with serous cystadenoma. Dendritic cells were isolated from peritoneal fluid, stained with monoclonal antibodies anti-BDCA-1 and anti-BDCA-2 and estimated using flow cytometry.
  
  
  RESULTS
  Peritoneal fluid myeloid DCs constituted 0.59% of mononuclear cells in patients with ovarian cancer and 7.2% in women with serous cystadenoma. Lymphoid DCs constituted 0.39% of PF mononuclears in women with ovarian cancer and 0.07% in patients with serous cystadenoma. The percentage of lymphoid DCs was higher in patients with ovarian cancer than in women with serous cystadenoma. The BDCA-1/BDCA-2 DCs ratio in peritoneal fluid of patients with serous cystadenoma was significantly higher in comparison to ovarian cancer.
  
  
  CONCLUSIONS
  Decreased BDCA-1/BDCA-2 DCs ratio in patients with ovarian cancer may favour Th2 lymphocyte differentiation and/or induction of immunological tolerance.
authors.affiliation: 1st Department of Gynecology, University School of Medicine, ul. Staszica 16, 20-081 Lublin, Poland. iwonawertel@wp.pl
authors.forename: F
authors.initials: F
authors.lastname: Wertel
authors.forename: G
authors.initials: G
authors.lastname: Polak
authors.forename: J
authors.initials: J
authors.lastname: Roliński
authors.forename: B
authors.initials: B
authors.lastname: Barczyński
authors.forename: J
authors.initials: J
authors.lastname: Kotarski
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001202:Ascitic Fluid / Q000166:cytology* / Q000276:immunology; D018284:Cystadenocarcinoma, Serous / Q000276:immunology / Q000473:pathology; D018293:Cystadenoma, Serous / Q000276:immunology / Q000473:pathology; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D037181:Lectins, C-Type / Q000032:analysis; D008214:Lymphocytes / Q000166:cytology; D008562:Membrane Glycoproteins / Q000032:analysis; D008875:Middle Aged; D022423:Myeloid Cells / Q000166:cytology; D010051:Ovarian Neoplasms / Q000276:immunology / Q000473:pathology*; D011971:Receptors, Immunologic / Q000032:analysis
chemical_list: C443299:CLEC4C protein, human; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic