Applications of fluorescence microscopy to single bacterial cells.
Fluorescence-imaging techniques are now being applied to single bacterial cells for the determination of in vivo kinase and transcription activity, protein-protein interactions, protein localization and protein mobility. Time-lapse imaging is being used at a population level to study heterogeneities, colony morphologies and the genetic networks underlying these phenomena. We will discuss here the possibilities, advantages and limitations of such techniques, focusing on the use of green fluorescent protein and its spectral variants.
Meyer
Pablo
P
Department of Microbiology, College of Physicians and Surgeons, Columbia University, 701 West 168th Street, Room 1218, New York, NY 10032, USA.
Dworkin
Jonathan
J
D001419:Bacteria / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins