title: Leucocyte antibodies in blood donors and a look back on recipients of their blood components.
abstract: OBJECTIVE
  The role of leucocyte antibodies in donors is poorly understood in pathogenesis of transfusion-related acute lung injury (TRALI). We examined antibodies in donors and traced recipients transfused with their blood components.
  
  
  METHODS
  Antibodies were examined in 1043 donors by five methods, look back performed in 26 recipients.
  
  
  RESULTS
  Anti-human leucocyte antigen detected by enzyme-linked immunosorbent assay in 9.8% women but none in men. Specificities identified using FlowPRA, antibodies detected after several months. TRALI reported in one recipient from immunized donor. In 11 of 26 recipients without TRALI, cognate antigens were identified.
  
  
  CONCLUSIONS
  Detection of antibodies in donors cannot predict TRALI, even in recipients with cognate antigen(s).
authors.affiliation: Institute of Haematology and Blood Transfusion, Warsaw, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Maślanka
authors.forename: H
authors.initials: H
authors.lastname: Michur
authors.forename: B
authors.initials: B
authors.lastname: Zupańska
authors.forename: M
authors.initials: M
authors.lastname: Uhrynowska
authors.forename: J
authors.initials: J
authors.lastname: Nowak
mesh_terms: D000328:Adult; D001782:Blood Donors; D005260:Female; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D007518:Isoantibodies / Q000009:adverse effects / Q000097:blood; D017708:Leukocyte Transfusion / Q000009:adverse effects*; D008297:Male; D008875:Middle Aged; D010298:Parity / Q000276:immunology*; D011247:Pregnancy; D012128:Respiratory Distress Syndrome / Q000276:immunology* / Q000517:prevention & control
chemical_list: D006680:HLA Antigens; D007518:Isoantibodies