title: Control of frictional coupling of transmembrane cell receptors in model cell membranes with linear polymer spacers.
abstract: The planar plasma membrane model with linear polymer spacers with defined lengths enables the control of the frictional coupling between incorporated transmembrane proteins (human platelet integrin) and the solid substrate. This mimics the viscous environment provided by the extracellular matrix of cells. The friction coefficient can be calculated quantitatively from the diffusion coefficient of integrin, measured by fluorescence recovery after photobleaching. The obtained results demonstrate a clear influence of the length and lateral density of polymer chains on the mobility of transmembrane proteins.
authors.affiliation: Lehrstuhl für Biophysik E22, Technische Universität München, D85748 Garching, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Purrucker
authors.forename: Anton
authors.initials: A
authors.lastname: Förtig
authors.forename: Rainer
authors.initials: R
authors.lastname: Jordan
authors.forename: Erich
authors.initials: E
authors.lastname: Sackmann
authors.forename: Motomu
authors.initials: M
authors.lastname: Tanaka
mesh_terms: D002462:Cell Membrane / Q000737:chemistry*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004058:Diffusion; D005109:Extracellular Matrix / Q000737:chemistry; D006801:Humans; D066298:In Vitro Techniques; D016023:Integrins / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry; D008956:Models, Chemical; D038761:Photobleaching; D011956:Receptors, Cell Surface; D013050:Spectrometry, Fluorescence; D014783:Viscosity
chemical_list: D016023:Integrins; D008565:Membrane Proteins; D011956:Receptors, Cell Surface