title: Large scale deletions in the Saccharomyces cerevisiae genome create strains with altered regulation of carbon metabolism.
abstract: Saccharomyces cerevisiae, for centuries the yeast that has been the workhorse for the fermentative production of ethanol, is now also a model system for biological research. The recent development of chromosome-splitting techniques has enabled the manipulation of the yeast genome on a large scale, and this has allowed us to explore questions with both biological and industrial relevance, the number of genes required for growth and the genome organization responsible for the ethanol production. To approach these questions, we successively deleted portions of the yeast genome and constructed a mutant that had lost about 5% of the genome and that gave an increased yield of ethanol and glycerol while showing levels of resistance to various stresses nearly equivalent to those of the parental strain. Further systematic deletion could lead to the formation of a eukaryotic cell with a minimum set of genes exhibiting appropriately altered regulation for enhanced metabolite production.
authors.affiliation: Department of Microbiology and Immunology, School of Medicine, Keio University, Shinjuku, Tokyo, 160-8582, Japan.
authors.forename: Kiriko
authors.initials: K
authors.lastname: Murakami
authors.forename: Eriko
authors.initials: E
authors.lastname: Tao
authors.forename: Yuki
authors.initials: Y
authors.lastname: Ito
authors.forename: Minetaka
authors.initials: M
authors.lastname: Sugiyama
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Kaneko
authors.forename: Satoshi
authors.initials: S
authors.lastname: Harashima
authors.forename: Takahiro
authors.initials: T
authors.lastname: Sumiya
authors.forename: Atsushi
authors.initials: A
authors.lastname: Nakamura
authors.forename: Masafumi
authors.initials: M
authors.lastname: Nishizawa
mesh_terms: D002244:Carbon / Q000378:metabolism*; D002872:Chromosome Deletion; D016681:Genome, Fungal; D005947:Glucose / Q000378:metabolism; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*
chemical_list: D002244:Carbon; D005947:Glucose