Measurement of salivary adiponectin levels.
We designed a method for measuring salivary adiponectin. In 188 healthy males, salivary adiponectin levels were measured using a commercial enzyme immunoassay kit for plasma with minor modifications. Intra- and inter-assay coefficients of variation for salivary adiponectin ranged from 0.6 to 4.9 and 1.1 to 9.8%, respectively. Salivary adiponectin levels ranged from 0.37 to 6.42 ng/ml, exceeding the kit's detection limit. For the over-43 age group, there was a significant correlation between plasma and salivary adiponectin levels (p<0.000001). These findings suggest the possibilities of salivary adiponectin as a marker of increased risk of non-insulin-dependent diabetes mellitus or cardiovascular disease.
Toda
M
M
Department of Social and Environmental Medicine, Osaka University Graduate School of Medicine, 2-2 Yamada-Oka, Suita, Osaka 565-0871, Japan.
Tsukinoki
R
R
Morimoto
K
K
D052242:Adiponectin / Q000032:analysis / Q000097:blood; D000328:Adult; D000367:Age Factors; D015415:Biomarkers / Q000032:analysis; D002318:Cardiovascular Diseases / Q000209:etiology; D019411:Clinical Laboratory Techniques; D003920:Diabetes Mellitus / Q000209:etiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D011933:Reagent Kits, Diagnostic; D012307:Risk Factors; D012463:Saliva / Q000737:chemistry*
C499918:ADIPOQ protein, human; D052242:Adiponectin; D015415:Biomarkers; D011933:Reagent Kits, Diagnostic