title: [Self-renewal signaling pathway and culture system in vitro of embryonic stem cells].
abstract: OBJECTIVE
  To review the latest development of the research on the self-renwal signaling pathway and culture system in vitro of the embryonic stem cells (ESCs).
  
  
  METHODS
  The recent articles about the self-renewal signaling pathway and culture system in vitro of the ESCs were extensively reviewed.
  
  
  RESULTS
  Understanding of the molecular mechanism of the self-renewal in vitro and pluripotency of the ESCs was considered important for developing improved methods of deriving, culturing and differentiating these cells into the cells that could be successfully used in the clinical practice.
  
  
  CONCLUSIONS
  A further research is needed to elucidate the self-renewal signaling pathway and the pluripotency of the ESCs and the culture system in vitro for the human ESCs remains to be further improved and developed.
authors.affiliation: Cancer Center of West China Hospital, Center of Reproductive Medicine, West China Second University Hospital, Sichuan University, Chengdu Sichuan, 610041, P. R. China. zhujiang1@medmail.com.cn
authors.forename: Jiang
authors.initials: J
authors.lastname: Zhu
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Yongshu
authors.initials: Y
authors.lastname: Diao
mesh_terms: D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D003470:Culture Media; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology; D006801:Humans; D053605:Leukemia Inhibitory Factor / Q000378:metabolism; D051379:Mice; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D051153:Wnt Proteins / Q000378:metabolism
chemical_list: D019485:Bone Morphogenetic Proteins; D003470:Culture Media; D053605:Leukemia Inhibitory Factor; D051153:Wnt Proteins; D019869:Phosphatidylinositol 3-Kinases