Assay methods for antihepatotoxic activity using peroxide-induced cytotoxicity in primary cultured hepatocytes1.
Conditions were investigated to devise IN VITRO assay methods for antihepatotoxic activity using peroxide-induced damage in primary cultured rat liver cells. Utilizing linoleic acid peroxide, benzoyl peroxide, cumene hydroperoxide and adenosine diphosphate/Fe (3+), satisfactory assay procedures were established. Some natural products known to exert liver-protective effects IN VIVO were screened to reveal that glycyrrhetinic acid, glycyrrhizin, cynarin, silybin and desoxypodophyllotoxin showed similar antihepatotoxic activity in these four assay methods.
Kiso
Y
Y
Pharmaceutical Institute, Tohoku University, Aoba-yama, Sendai, Japan.
Kato
O
O
Hikino
H
H