Enhanced electrophoretic resolution of monosulfate glycosaminoglycan disaccharide isomers on poly(methyl methacrylate) chips.
To improve the separation of monosulfate glycosaminoglycan disaccharide isomers by microchip electrophoresis, we found that addition of 1,4-dioxane (DO) dramatically improved analyte resolution, probably due to solvation effects. Methylcellulose (MC) was tested for the ability to suppress EOF and analyte adsorption to the chip. To improve analyte resolution, buffer pH, beta-CD, and DO were systematically investigated. Fast separation was achieved by increasing the electric field strength, and field-amplified sample stacking occurred with increasing buffer concentrations. Therefore, based on our findings, we describe an efficient method for the separation of monosulfate and trisulfate unsaturated disaccharides (DeltaDi-UA2S, DeltaDi-4S, DeltaDi-6S, and DeltaDi-triS) derivatized with 2-aminoacridone hydrochloride. A mixture of monosulfate disaccharide isomers (DeltaDi-UA2S, DeltaDi-4S, and DeltaDi-6S) was baseline-separated within 75 s on a poly(methyl methacrylate) chip using a mixed buffer (DO/running buffer 57:43 v:v), 0.5% MC, pH 6.81, with an E(sep) of 558 V/cm. The theoretical plate was in the range of 5 x 10(5) to 1 x 10(6) m-1.
Zhang
Yong
Y
Graduate School of Pharmaceutical Sciences, the University of Tokushima, Tokushima, Japan. yl7173@gmail.com
Ping
Guichen
G
Zhu
Bingmei
B
Kaji
Noritada
N
Tokeshi
Manabu
M
Baba
Yoshinobu
Y
D000609:Aminoacridines / Q000737:chemistry; D002021:Buffers; D004146:Dioxanes / Q000737:chemistry; D004187:Disaccharides / Q000032:analysis*; D046209:Electrophoresis, Microchip / Q000379:methods*; D006025:Glycosaminoglycans / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007536:Isomerism; D008747:Methylcellulose / Q000737:chemistry; D019904:Polymethyl Methacrylate; D047392:beta-Cyclodextrins
D000609:Aminoacridines; D002021:Buffers; D004146:Dioxanes; D004187:Disaccharides; D006025:Glycosaminoglycans; D047392:beta-Cyclodextrins; C072515:2-aminoacridone; D008747:Methylcellulose; D019904:Polymethyl Methacrylate; C025223:1,4-dioxane