[Effects of keratinocyte growth factor on the expression and activity of extracellular signal-regulated kinase in Hep-2 cell line].
OBJECTIVE
To observe effects of keratinocyte growth factor (KGF) on the proliferation of Hep-2 cell line; the activity and expression of extracellular signal-regulated kinase (ERK).


METHODS
Cell line proliferation was measured by Flow cytometry and MTT assay; ERK activity was measured by ERKl/2 immuno-precipitation kinase assay kit; and p ERK expression was assessed by Western blot.


RESULTS
KGF can improve the proliferation of Hep-2 cell line. The intracellular ERK activity and p-ERK expression were increased by different concentrations of KGF.


CONCLUSIONS
KGF can stimulated proliferation of Hep-2 cell line with enhancing activity of ERK and expression of p-ERK.
Fang
Rong
R
Department of Otolaryngology, the First People's Hospital of Yichang, Yichang, 443003, China.
Hu
Jin
J
Zhen
Hongtao
H
Cui
Yonghua
Y
D045744:Cell Line, Tumor; D049109:Cell Proliferation; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D051523:Fibroblast Growth Factor 7 / Q000494:pharmacology*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D015398:Signal Transduction
D051523:Fibroblast Growth Factor 7; D048049:Extracellular Signal-Regulated MAP Kinases