title: Influence of novel resin monomer on viability of L-929 mouse fibroblasts in vitro.
abstract: We have previously synthesized a novel acrylic resin monomer, methacryloyloxyethyl methyl succinate (TA). The aim of this in vitro study, therefore, was to examine its influence on cell viability using L-929 mouse fibroblasts and then compare the results with MMA, EMA, and LMA. Medium containing each monomer was changed every 15 minutes as some monomers were volatile. After one hour of exposure, these mediums were replaced with a normal medium and cells were further incubated for 72 hours. IC50 value for each monomer was determined, and chronological cell viability and cytomorphologic observation were evaluated. Viability was impaired in a dose-dependent manner. All monomers, except TA, tended to correlate between molecular weight and cell viability. On the other hand, TA showed excellent viability and did not impair growth abruptly. These results thus demonstrated that cellular damage by TA was much lower than that by other monomers.
authors.affiliation: Department of Periodontology, School of Dentistry, Aichi-Gakuin University, 2-11 Suemori-dori, Chikusa-ku, Nagoya 464-8651, Japan. sjin@sdent.aichi-gakuin.ac.jp
authors.forename: Satoshi
authors.initials: S
authors.lastname: Jinno
authors.forename: Tatsushi
authors.initials: T
authors.lastname: Kawai
authors.forename: Atsuko
authors.initials: A
authors.lastname: Ishikawa
authors.forename: Tomoo
authors.initials: T
authors.lastname: Suzuki
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Hattori
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Okeya
authors.forename: Tatsuhide
authors.initials: T
authors.lastname: Hayashi
authors.forename: Hatsuhiko
authors.initials: H
authors.lastname: Maeda
authors.forename: Yuzo
authors.initials: Y
authors.lastname: Ohno
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Ito
authors.forename: Toshihide
authors.initials: T
authors.lastname: Noguchi
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry* / Q000633:toxicity*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects*; D020128:Inhibitory Concentration 50; D007739:L Cells; D051379:Mice; D008970:Molecular Weight; D013997:Time Factors; D014835:Volatilization
chemical_list: D000180:Acrylic Resins