title: T-independent activation-induced cytidine deaminase expression, class-switch recombination, and antibody production by immature/transitional 1 B cells.
abstract: Inflammation elicits a splenic lymphopoiesis of unknown physiologic significance but one that juxtaposes developing B cells and exogenous Ag. We show that immature and transitional 1 (immature/T1) B cells constitutively express activation-induced cytidine deaminase and B lymphocyte-induced maturation protein 1 in amounts that support accelerated plasmacytic differentiation and limited class-switch recombination. In vivo, activation of immature/T1 B cells by TLR ligands or bacterial vaccine rapidly induces T1 cells to divide, proliferate, and secrete IgM, IgG, or IgA Ab; in vitro, proliferation and differentiation are substantially enhanced by B cell-activating factor. We propose that inflammation-induced extramedullary lymphopoiesis represents a specialized mechanism for innate Ab responses to microbial pathogens.
authors.affiliation: Department of Immunology, Duke University Medical Center, Durham, NC 27710, USA.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ueda
authors.forename: Dongmei
authors.initials: D
authors.lastname: Liao
authors.forename: Kaiyong
authors.initials: K
authors.lastname: Yang
authors.forename: Anjali
authors.initials: A
authors.lastname: Patel
authors.forename: Garnett
authors.initials: G
authors.lastname: Kelsoe
mesh_terms: D000818:Animals; D000917:Antibody Formation; D053264:B-Cell Activating Factor / Q000096:biosynthesis / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology*; D002455:Cell Division / Q000276:immunology; D003564:Cytidine Deaminase / Q000096:biosynthesis* / Q000276:immunology; D015971:Gene Expression Regulation, Enzymologic / Q000276:immunology*; D007113:Immunity, Innate; D017578:Immunoglobulin Class Switching / Q000276:immunology*; D007136:Immunoglobulins / Q000096:biosynthesis / Q000276:immunology; D038041:Lymphopoiesis / Q000276:immunology; D051379:Mice; D018345:Mice, Knockout; D008819:Mice, Nude; D010950:Plasma Cells / Q000276:immunology* / Q000378:metabolism; D013154:Spleen / Q000276:immunology / Q000378:metabolism; D051193:Toll-Like Receptors / Q000819:agonists / Q000276:immunology; D000098830:AICDA (Activation-Induced Cytidine Deaminase)
chemical_list: D053264:B-Cell Activating Factor; D001428:Bacterial Vaccines; D007136:Immunoglobulins; D051193:Toll-Like Receptors; D000098830:AICDA (Activation-Induced Cytidine Deaminase); D003564:Cytidine Deaminase