Development and clinical evaluation of a PCR assay targeting the metalloprotease gene (mprA) of B. pseudomallei.
A PCR assay targeting the metalloprotease gene (mprA) of Burkholderia pseudomallei was developed for the specific detection of this organism in pure cultures and clinical samples. All other closely related organisms including B. mallei the causative agent of glanders, and B. thailandensis tested negative. Burkholderia pseudomallei DNA was successfully amplified from paraffin-embedded lung tissue of a camel with a generalized B. pseudomallei infection. The developed PCR assay can be used as a simple tool for the specific and sensitive detection of B. pseudomallei.
Neubauer
H
H
Friedrich-Loeffler-Institut, Naumburger Str. 96a, D-07743 Jena, Germany. heinrich.neubauer@fli.bund.de
Sprague
L D
LD
Joseph
M
M
Tomaso
H
H
Al Dahouk
S
S
Witte
A
A
Kinne
J
J
Hensel
A
A
Wernery
R
R
Wernery
U
U
Scholz
H C
HC
D000818:Animals; D001483:Base Sequence; D016957:Burkholderia pseudomallei / Q000302:isolation & purification*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005784:Gene Amplification; D006801:Humans; D045726:Metalloproteases / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity; D013045:Species Specificity
D017931:DNA Primers; D004269:DNA, Bacterial; D045726:Metalloproteases