title: Myosin Va maneuvers through actin intersections and diffuses along microtubules.
abstract: Certain types of intracellular organelle transport to the cell periphery are thought to involve long-range movement on microtubules by kinesin with subsequent handoff to vertebrate myosin Va (myoVa) for local delivery on actin tracks. This process may involve direct interactions between these two processive motors. Here we demonstrate using single molecule in vitro techniques that myoVa is flexible enough to effectively maneuver its way through actin filament intersections and Arp2/3 branches. In addition, myoVa surprisingly undergoes a one-dimensional diffusive search along microtubules, which may allow it to scan efficiently for kinesin and/or its cargo. These features of myoVa may help ensure efficient cargo delivery from the cell center to the periphery.
authors.affiliation: Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, VT 05405, USA.
authors.forename: M Yusuf
authors.initials: MY
authors.lastname: Ali
authors.forename: Elena B
authors.initials: EB
authors.lastname: Krementsova
authors.forename: Guy G
authors.initials: GG
authors.lastname: Kennedy
authors.forename: Rachel
authors.initials: R
authors.lastname: Mahaffy
authors.forename: Thomas D
authors.initials: TD
authors.lastname: Pollard
authors.forename: Kathleen M
authors.initials: KM
authors.lastname: Trybus
authors.forename: David M
authors.initials: DM
authors.lastname: Warshaw
mesh_terms: D000199:Actins / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002645:Chickens; D003599:Cytoskeleton / Q000378:metabolism; D004058:Diffusion; D007091:Image Processing, Computer-Assisted; D016547:Kinesins / Q000737:chemistry; D008870:Microtubules / Q000378:metabolism*; D008954:Models, Biological; D020409:Molecular Motor Proteins / Q000737:chemistry; D024701:Myosin Type V / Q000737:chemistry* / Q000378:metabolism*; D009218:Myosins / Q000737:chemistry
chemical_list: D000199:Actins; D020409:Molecular Motor Proteins; D024701:Myosin Type V; D009218:Myosins; D016547:Kinesins