title: NPY in rat retina is present in neurons, in endothelial cells and also in microglial and Müller cells.
abstract: NPY is present in the retina of different species but its role is not elucidated yet. In this work, using different rat retina in vitro models (whole retina, retinal cells in culture, microglial cell cultures, rat Müller cell line and retina endothelial cell line), we demonstrated that NPY staining is present in the retina in different cell types: neurons, macroglial, microglial and endothelial cells. Retinal cells in culture express NPY Y(1), Y(2), Y(4) and Y(5) receptors. Retina endothelial cells express all NPY receptors except NPY Y(5) receptor. Moreover, NPY is released from retinal cells in culture upon depolarization. In this study we showed for the first time that NPY is present in rat retina microglial cells and also in rat Müller cells. These in vitro models may open new perspectives to study the physiology and the potential pathophysiological role of NPY in the retina.
authors.affiliation: Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.
authors.forename: Ana Rita
authors.initials: AR
authors.lastname: Alvaro
authors.forename: Joana
authors.initials: J
authors.lastname: Rosmaninho-Salgado
authors.forename: Ana Raquel
authors.initials: AR
authors.lastname: Santiago
authors.forename: João
authors.initials: J
authors.lastname: Martins
authors.forename: Célia
authors.initials: C
authors.lastname: Aveleira
authors.forename: Paulo F
authors.initials: PF
authors.lastname: Santos
authors.forename: Tiago
authors.initials: T
authors.lastname: Pereira
authors.forename: Denisa
authors.initials: D
authors.lastname: Gouveia
authors.forename: Ana Luísa
authors.initials: AL
authors.lastname: Carvalho
authors.forename: Eric
authors.initials: E
authors.lastname: Grouzmann
authors.forename: António Francisco
authors.initials: AF
authors.lastname: Ambrósio
authors.forename: Cláudia
authors.initials: C
authors.lastname: Cavadas
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000737:chemistry*; D017628:Microglia / Q000737:chemistry*; D009478:Neuropeptide Y / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger; D051381:Rats; D017208:Rats, Wistar; D017476:Receptors, Neuropeptide Y / Q000032:analysis / Q000235:genetics; D012160:Retina / Q000737:chemistry* / Q000166:cytology; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D009478:Neuropeptide Y; D012333:RNA, Messenger; D017476:Receptors, Neuropeptide Y