Human monoclonal antibody against Hepatitis B virus surface antigen (HBsAg).
Hepatitis B virus (HBV) is one of the main pathogens responsible for hepatitis and hepatocellular carcinoma. Human plasma-derived Hepatitis B immune globulin (HBIG) is being used for prophylactic and liver transplantation currently. However, it may be necessary to replace a HBIG with a recombinant one because of limited availability of human plasma with high anti-HBsAg antibody titer and possible contamination of human pathogens. A Chinese hamster ovary (CHO) cell line, HB-C7A, was established which produces a fully human IgG1 that binds HBsAg. The HB-C7A exhibits approximately 2600 units/mg of antibody. The affinity (K(a)) of HB-C7A is 1.1 x 10(8) M(-1) by Biacore analysis and estimated 6.7-fold higher than that of Hepabig (a plasma-derived HBIG from Green Cross Corp., Yongin, Korea) by competition ELISA. The HB-C7A recognizes the conformational "a" determinant of HBsAg and binds HBV particle more efficiently than the Hepabig. The HB-C7A binds to HBV-infected human liver tissue but does not bind to normal human tissues. This HB-C7A has several advantages compared to plasma-derived Hepabig such as activity, safety and availability.
Shin
Yong-Won
YW
Antibody Engineering Laboratory, Research Center, Green Cross Corporation, 341 Bojeong-Dong, Giheung-Gu, Yongin City, Gyunggi-Do 446-799, Republic of Korea.
Ryoo
Kyung-Hwan
KH
Hong
Kwang-Won
KW
Chang
Ki-Hwan
KH
Choi
Jin-Seol
JS
So
Minyoung
M
Kim
Pan-Kyung
PK
Park
Jie-Young
JY
Bong
Ki-Tae
KT
Kim
Se-Ho
SH
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D000915:Antibody Affinity / Q000276:immunology; D000937:Antigen-Antibody Reactions / Q000276:immunology; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D003429:Cross Reactions / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D006509:Hepatitis B / Q000276:immunology* / Q000821:virology; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D006515:Hepatitis B virus / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D008099:Liver / Q000276:immunology / Q000821:virology; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D006514:Hepatitis B Surface Antigens; D007074:Immunoglobulin G; D011994:Recombinant Proteins