Definition of the USDA103 strain of channel catfish (Ictalurus punctatus).
Although there are differences in performance between genetic groups of channel catfish, identification and management of these groups is difficult because catfish strains look alike and individuals cannot be tagged efficiently. Thus, US catfish producers have not been able to objectively identify fish from different strains or populations, and it has been difficult for them to maintain the genetic purity of populations on the farm. We have developed a multiplexed microsatellite genotyping system to define catfish populations based on allelic frequency and exclusion. A commercial catfish genotype database was developed using catfish samples collected from 24 processing plants in the four main US catfish-producing states. The utility of the system was tested by the molecular characterization of the USDA103 research strain. Using eight microsatellite loci, the probability of falsely classifying an individual non-USDA103 catfish as a USDA103 was 0.0065. From a sample of 50 fish from a putative USDA103 pond, the probability of falsely including two non-USDA103 fish was 1 x 10(-105), and the conservative probability of falsely excluding two USDA103 fish was 1 x 10(-6). This genotyping system provides channel catfish producers with an objective mechanism for identification and management of genetically selected fish.
Waldbieser
G C
GC
USDA, Agricultural Research Service, Catfish Genetics Research Unit, 141 Experiment Station Road, Stoneville, MS, USA. geoff.waldbieser@ars.usda.gov
Wolters
W R
WR
D000818:Animals; D001947:Breeding / Q000379:methods*; D017931:DNA Primers; D030541:Databases, Genetic; D005787:Gene Frequency; D005821:Genetic Techniques; D005838:Genotype; D007059:Ictaluridae / Q000145:classification* / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics; D013045:Species Specificity
D017931:DNA Primers