title: Identification of bovine QTL for growth and carcass traits in Japanese Black cattle by replication and identical-by-descent mapping.
abstract: To map quantitative trait loci (QTL) for growth and carcass traits in a purebred Japanese Black cattle population, we conducted multiple QTL analyses using 15 paternal half-sib families comprising 7860 offspring. We identified 40 QTL with significant linkages at false discovery rates of less than 0.1, which included 12 for intramuscular fat deposition called marbling and 12 for cold carcass weight or body weight. The QTL each explained 2%-13% of the phenotypic variance. These QTL included many replications and shared hypothetical identical-by-descent (IBD) alleles. The QTL for CW on BTA14 was replicated in five families with significant linkages and in two families with a 1% chromosome-wise significance level. The seven sires shared a 1.1-Mb superior Q haplotype as a hypothetical IBD allele that corresponds to the critical region previously refined by linkage disequilibrium mapping. The QTL for marbling on BTA4 was replicated in two families with significant linkages. The QTL for marbling on BTA6, 7, 9, 10, 20, and 21 and the QTL for body weight on BTA6 were replicated with 1% and/or 5% chromosome-wise significance levels. There were shared IBD Q or q haplotypes in the marbling QTL on BTA4, 6, and 10. The allele substitution effect of these haplotypes ranged from 0.7 to 1.2, and an additive effect between the marbling QTL on BTA6 and 10 was observed in the family examined. The abundant and replicated QTL information will enhance the opportunities for positional cloning of causative genes for the quantitative traits and efficient breeding using marker-assisted selection.
authors.affiliation: Shirakawa Institute of Animal Genetics, Japan Livestock Technology Association, Odakura, Nishigo, Fukushima 961-8061, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Takasuga
authors.forename: Toshio
authors.initials: T
authors.lastname: Watanabe
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Mizoguchi
authors.forename: Takashi
authors.initials: T
authors.lastname: Hirano
authors.forename: Naoya
authors.initials: N
authors.lastname: Ihara
authors.forename: Atsushi
authors.initials: A
authors.lastname: Takano
authors.forename: Kou
authors.initials: K
authors.lastname: Yokouchi
authors.forename: Akira
authors.initials: A
authors.lastname: Fujikawa
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Chiba
authors.forename: Naohiko
authors.initials: N
authors.lastname: Kobayashi
authors.forename: Ken
authors.initials: K
authors.lastname: Tatsuda
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Oe
authors.forename: Megumi
authors.initials: M
authors.lastname: Furukawa-Kuroiwa
authors.forename: Atsuko
authors.initials: A
authors.lastname: Nishimura-Abe
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Fujita
authors.forename: Kazuya
authors.initials: K
authors.lastname: Inoue
authors.forename: Kazunori
authors.initials: K
authors.lastname: Mizoshita
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ogino
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Sugimoto
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics* / Q000254:growth & development; D002874:Chromosome Mapping; D003198:Computer Simulation; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D016678:Genome; D006128:Growth / Q000235:genetics*; D007564:Japan; D040641:Quantitative Trait Loci; D012098:Reproduction / Q000235:genetics*
chemical_list: D004247:DNA