Localized mutagenesis.
Localized mutagenesis can be used to obtain mutants in genes of interest based on linkage to selectable markers. Mutagens diethylsulfate and hydroxylamine are used to obtain predominantly transition mutations in the DNA either by whole chromosomal mutagenesis or mutagenesis of DNA isolated as purified plasmid or packaged in transducing phage particles. Selectable markers can include those based on auxotrophic requirements, carbon or nitrogen source utilization, or antibiotic resistance markers, such as those encoded in transposons.
Maloy
Stanley R
SR
Department of Biology, Center for Microbial Sciences, San Diego State University, San Diego, CA, USA.
D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D016296:Mutagenesis; D009153:Mutagens; D009154:Mutation
D004269:DNA, Bacterial; D009153:Mutagens