title: Correlation of photodynamic activity and fluorescence signaling for free and pegylated mTHPC in mesothelioma xenografts.
abstract: OBJECTIVE
  Correlation of photodynamic activity (PDT) and fluorescence signaling for free and pegylated meta-tetrahydroxyphenylchlorin (mTHPC) in nude mice with mesothelioma xenografts.
  
  
  METHODS
  Twelve animals received light delivery (20 J/cm(2), 150 mW/cm(2), spot size 1.2 cm) on the tumor and the hind leg 3 days after sensitization with 0.15 mg/kg free mTHPC (n = 6) or equimolar-dosed pegylated mTHPC (n = 6). Groups of three animals each were sensitized with 0.15 and 0.5 mg/kg free mTHPC or equimolar dosed pegylated mTHPC followed after 3 days by fluorescence microscopy measurements.
  
  
  RESULTS
  Pegylated mTHPC resulted in a similar extent of PDT-related tumor necrosis but in lower skin phototoxicity than free mTHPC. Both mTHPC formulations were heterogeneously distributed in the tumor and were mainly localized in perivascular areas. Pegylated mTHPC revealed a higher tumor to skin fluorescence intensity ratio than free mTHPC (P<0.001).
  
  
  CONCLUSIONS
  Fluorescence signaling measurement has the potential to predict the photodynamic activity for both mTHPC formulations in mesothelioma xenografts.
authors.affiliation: Division of Thoracic Surgery, University of Lausanne, Lausanne, Switzerland.
authors.forename: Nam
authors.initials: N
authors.lastname: Tran
authors.forename: Thorsten
authors.initials: T
authors.lastname: Krueger
authors.forename: Youmin
authors.initials: Y
authors.lastname: Pan
authors.forename: Hua
authors.initials: H
authors.lastname: Yan
authors.forename: Cai
authors.initials: C
authors.lastname: Cheng
authors.forename: Hans-Jörg
authors.initials: HJ
authors.lastname: Altermatt
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Ballini
authors.forename: François
authors.initials: F
authors.lastname: Borle
authors.forename: Hans-Beat
authors.initials: HB
authors.lastname: Ris
authors.forename: Snezana
authors.initials: S
authors.lastname: Andrejevic-Blant
mesh_terms: D000818:Animals; D005260:Female; D008652:Mesoporphyrins / Q000494:pharmacology*; D008654:Mesothelioma / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D008856:Microscopy, Fluorescence; D009336:Necrosis; D009368:Neoplasm Transplantation; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology*; D010997:Pleural Neoplasms / Q000188:drug therapy* / Q000473:pathology; D014183:Transplantation, Heterologous
chemical_list: D008652:Mesoporphyrins; D017319:Photosensitizing Agents; C072269:temoporfin