Separation of enantiomers of deprenyl with various CDs in CE and the effect of enantiomer migration order on enantiomeric impurity determination of selegiline in active ingredients and tablets.
Opposite affinity pattern of enantiomers of the antiparkinsonian chiral drug deprenyl (DEP) was observed towards various neutral and charged derivatives of -CD. The effect of the enantiomer migration order on the LOD of enantiomeric impurity of R-DEP (selegiline) was studied for the standard substances and in the tablets from three different suppliers. The influence of injection mode on the LOD of a minor enantiomeric impurity was also studied and the CE method was compared with the pharmacopoeial HPLC method using a commercially available chiral column Chiralcel OD-H. The optimized CE method was more suitable for low-level enantiomeric impurity determination in selegiline compared to the pharmacopoeial HPLC method.
Castro-Puyana
María
M
Departamento de Química Analítica, Facultad de Química, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain.
Lomsadze
Ketevan
K
LCrego
Antonio
A
Marina
María Luisa
ML
Chankvetadze
Bezhan
B
D000978:Antiparkinson Agents / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary; D012642:Selegiline / Q000032:analysis* / Q000737:chemistry; D013237:Stereoisomerism; D013607:Tablets; D047392:beta-Cyclodextrins / Q000737:chemistry*
D000978:Antiparkinson Agents; D013607:Tablets; D047392:beta-Cyclodextrins; D012642:Selegiline