[Use of monoclonal antibodies against horse immunoglobulin in an enzyme immunoassay of bacterial toxins and anatoxins].
Immunization of BALB/c mice by horse antiserum against diphtheria made it possible to obtain IgG1 monoclonal antibodies (MoAbs) 2B7E4 specific for light chains of horse immunoglobulin (Ig). Unlike commercial preparations of anti-horse immunoglobulin antibodies, which are specific for the whole Ig molecule or its Fc-fragment, the peroxidase (HRP) conjugate of the MoAb, 2B7E4-HRP did not interact with human, mouse, rabbit, and sheep Igs, or horse albumin. The conjugate obtained was used with MoAbs against bacterial toxins and commercial horse anatoxins, as a universal reagent in sandwich enzyme immunoassay (ELISA) for bacterial toxins and anatoxins. The detection sensitivity of diphtheria toxin/anatoxin equaled 0.0005 Lf/ml; tetanus toxin and anatoxin were detected with sensitivities of 20 LD50/ml and 0.005 UI/ml, respectively. A similar sandwich ELISA for botulinum anatoxins (group measurement) allowed types A, B, and E to be detected at 0.02, 0.002, and 0.001 UI/ml, respectively; selective measurement was only possible in the case of type E anatoxin (0.001 UI/ml).
Burkin
M A
MA
Gal'vidis
I A
IA
Iakovleva
I V
IV
Sviridov
V V
VV
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity; D001427:Bacterial Toxins / Q000032:analysis*; D001905:Botulinum Toxins / Q000032:analysis; D004167:Diphtheria Toxin / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D006735:Horseradish Peroxidase; D006736:Horses; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology*; D007147:Immunoglobulin Light Chains / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012680:Sensitivity and Specificity; D013744:Tetanus Toxin / Q000032:analysis; D014121:Toxoids / Q000032:analysis*
D000911:Antibodies, Monoclonal; D001427:Bacterial Toxins; D004167:Diphtheria Toxin; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007147:Immunoglobulin Light Chains; D013744:Tetanus Toxin; D014121:Toxoids; D006735:Horseradish Peroxidase; D001905:Botulinum Toxins