Effect of Plasmodium yoelii nigeriensis infection on hepatic and splenic glutathione-S-transferase(s) in Swiss albino and db/+ mice: efficacy of mefloquine and menadione in antimalarial chemotherapy.
The present report deals with the status of hepatic and splenic glutathione-S-transferase (GST) activities in mice during experimental infection with Plasmodium yoelii nigeriensis and subsequent treatment of infected mice with mefloquine (Mf) and menadione (Md). The infection caused significant decline in the hepatic and splenic glutathione-S-transferase (GST) activities of albino and db/+ mice. The decline was observed in the levels of both cytosolic and microsomal GST(s) of liver and spleen in both types of mice. Intraperitoneal administration of mefloquine at a dose of 5 mg/kg and menadione at a dose of 100 mg/kg, twice daily from day 1 p.i. (day 0) until day 10, caused restoration in the levels of hepatic as well as splenic GST(s), albeit to varying degrees. Mf was able to suppress parasitaemia by day 5 in the case of albino mice and by day 3 in the case of db/+ mice but was unable to cure both types of mice completely. On the other hand, Md caused a delay in maturation of infection in both cases, but could not cure the mice.
Ahmad
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R
Division of Biochemistry, PO Box No. 173, Central Drug Research Institute, Chattar Manzil Palace, Lucknow-226001, India.
Srivastava
A K
AK
D000818:Animals; D000933:Antifibrinolytic Agents / Q000008:administration & dosage / Q000494:pharmacology; D000962:Antimalarials / Q000008:administration & dosage / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000032:analysis / Q000187:drug effects*; D007274:Injections, Intraperitoneal; D008099:Liver / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology; D008288:Malaria / Q000188:drug therapy* / Q000401:mortality; D015767:Mefloquine / Q000008:administration & dosage / Q000494:pharmacology*; D051379:Mice; D008861:Microsomes / Q000187:drug effects / Q000201:enzymology; D018512:Parasitemia / Q000188:drug therapy / Q000201:enzymology; D010967:Plasmodium yoelii / Q000472:pathogenicity*; D011506:Proteins / Q000032:analysis; D013154:Spleen / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology; D013997:Time Factors; D024483:Vitamin K 3 / Q000008:administration & dosage / Q000494:pharmacology*
D000933:Antifibrinolytic Agents; D000962:Antimalarials; D011506:Proteins; D024483:Vitamin K 3; D005982:Glutathione Transferase; D015767:Mefloquine