title: Random access three-dimensional two-photon microscopy.
abstract: We propose a two-photon microscope scheme capable of real-time, three-dimensional investigation of the electric activity pattern of neural networks or signal summation rules of individual neurons in a 0.6 mm x 0.6 mm x 0.2 mm volume of the sample. The points of measurement are chosen according to a conventional scanning two-photon image, and they are addressed by separately adjustable optical fibers. This allows scanning at kilohertz repetition rates of as many as 100 data points. Submicrometer spatial resolution is maintained during the measurement similarly to conventional two-photon microscopy.
authors.affiliation: Department of Pharmacology, Institute of Experimental Medicine, Hungary.
authors.forename: Balázs
authors.initials: B
authors.lastname: Rózsa
authors.forename: Gergely
authors.initials: G
authors.lastname: Katona
authors.forename: E Sylvester
authors.initials: ES
authors.lastname: Vizi
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Várallyay
authors.forename: Attila
authors.initials: A
authors.lastname: Sághy
authors.forename: Lásló
authors.initials: L
authors.lastname: Valenta
authors.forename: Pál
authors.initials: P
authors.lastname: Maák
authors.forename: Júlia
authors.initials: J
authors.lastname: Fekete
authors.forename: Akos
authors.initials: A
authors.lastname: Bányász
authors.forename: Róbert
authors.initials: R
authors.lastname: Szipocs
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D002478:Cells, Cultured; D005336:Fiber Optic Technology / Q000295:instrumentation*; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000295:instrumentation* / Q000379:methods; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation / Q000379:methods*; D009415:Nerve Net / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D055100:Optical Fibers