title: Application of high-speed counter-current chromatography for the isolation of 9'-cis-neoxanthin from fresh spinach.
abstract: Preparative HSCCC (high-speed counter-current chromatography) could be applied for the isolation of 9'-cis-neoxanthin from a crude carotenoid extract of fresh spinach leaves. The separation was performed on a Pharma-Tech Research Corp. CCC 1000 with a solvent system composed of hexane:ethanol:water at a volume ratio of 5:5:4.5 at a flow rate of 3 mL/min and at 850 rpm, using the lower phase as mobile phase. 9'-cis-neoxanthin with a purity of up to 94% could be obtained with a single HSCCC purification step of the crude carotenoid extract.
authors.affiliation: Institute of Food Chemistry, Technical University of Braunschweig, Schleinitzstrasse 20, 38106 Braunschweig, Germany. s.baldermann@tu-bs.de
authors.forename: Susanne
authors.initials: S
authors.lastname: Baldermann
authors.forename: Annika
authors.initials: A
authors.lastname: Reinhard
authors.forename: Nils
authors.initials: N
authors.lastname: Köhler
authors.forename: Peter
authors.initials: P
authors.lastname: Fleischmann
mesh_terms: D003377:Countercurrent Distribution / Q000379:methods*; D015394:Molecular Structure; D015203:Reproducibility of Results; D018724:Spinacia oleracea / Q000737:chemistry*; D024341:Xanthophylls / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D024341:Xanthophylls; C011947:neoxanthin