Highly induced DNA recombination mediated by membrane permeabilized recombinant cre protein in mouse primary cells.
To establish efficient induction of Cre mediated DNA recombination in primary cells, mouse embryonic fibroblast, keratinocyte, and primary preosteoblast, we tested various recombinant Cres by fusing of protein transduction domain in human immunodeficiency virus (HIV) transactivator of transcription (TAT-PTD) to the N- and/or C-terminus. HTC, modified Cre with PTD at the N-terminus, achieved the highest activity of DNA recombination for those primary cells.
Hidema
Shizu
S
Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan.
Maruyama
Takamitsu
T
Kato
Shigeki
S
Nishimori
Katsuhiko
K
D000818:Animals; D018929:Cell Culture Techniques; D002463:Cell Membrane Permeability / Q000502:physiology*; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000166:cytology; D005347:Fibroblasts / Q000378:metabolism; D015696:Gene Products, tat; D019426:Integrases / Q000235:genetics / Q000378:metabolism*; D015603:Keratinocytes / Q000378:metabolism*; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism; D017434:Protein Structure, Tertiary / Q000235:genetics / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic / Q000235:genetics / Q000502:physiology*; D001616:beta-Galactosidase / Q000378:metabolism
D015696:Gene Products, tat; D011993:Recombinant Fusion Proteins; D004247:DNA; C045073:Cre recombinase; D019426:Integrases; D001616:beta-Galactosidase