Integrated microfluidic device with an electroplated palladium decoupler for more sensitive amperometric detection of the 8-hydroxy-deoxyguanosine (8-OH-dG) DNA adduct.
8-hydroxy-deoxyguanosine (8-OH-dG) DNA adduct is one of the most frequently used biomarkers reporting on the oxidative stress that leads to DNA damage. More sensitive and reliable microfluidic devices are needed for the detection of these biomarkers of interest. We have developed a capillary electrophoresis (CE)-based microfluidic device with an electroplated palladium decoupler that provides significantly improved detection limit, separation efficiency, and resolving power. The poly(dimethylsiloxane) (PDMS)/glass hybrid device has fully integrated gold microelectrodes covered in situ with palladium nanoparticles using an electroplating technique. The performance and coverage of the electrodes electroplated with palladium particles were evaluated electrochemically and via scanning electron microscope (SEM) imaging, respectively. The performance of the device was tested and evaluated with different buffer systems, pH values, and electric field strengths. The results showed that this device has significantly improved resolving power, even at separation electric field strengths as high as 600 V cm-1. The detection limit for the 8-OH-dG adduct is about 20 attomoles; the concentration limit is on the order of 100 nM (S/N=3). A linear response is reported for both 8-OH-dG and dG in the range from 100 nM to 150 microM (approximately 100 pA microM-1) with separation efficiencies of approximately 120,000-170,000 plates m-1.
Dawoud
Abdulilah A
AA
Department of Chemistry, Iowa State University, Ames, IA 50011, USA. adawoud@iastate.edu
Kawaguchi
Toshikazo
T
Jankowiak
Ryszard
R
D000080242:8-Hydroxy-2'-Deoxyguanosine; D018736:DNA Adducts / Q000737:chemistry / Q000302:isolation & purification*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004563:Electrochemistry; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D005898:Glass / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D008855:Microscopy, Electron, Scanning; D010084:Oxidation-Reduction; D010165:Palladium / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012828:Silicones / Q000737:chemistry; D013499:Surface Properties
D018736:DNA Adducts; D004129:Dimethylpolysiloxanes; D012828:Silicones; D010165:Palladium; C013830:baysilon; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine