title: [Experimental evaluation of the biological risks of introduction of the genetically modified microorganism (GMM) B. subtilis VKPM B-7092 into the environment].
abstract: Experimental evaluation of the biological risks of introducing the genetically modified microorganism (GMM) B. subtilis VKPM B-7092, an active ingredient of the probiotic VETOM 1.1, into an open system was performed. The following features of the GMM were studied: the survival rate of the GMM in bovine gastroenteric tract; its influence on the microbiocenosis; the species composition of microflora of the gastroenteric tract of the animal species; the possibility of transfer of the DNA fragment cloned in the B. subtilis bacterium and containing the gene of human leukocyte alpha2 interferon to the representatives of intestinal microflora of animals fed on the probiotic VETOM 1.1, as well as the GMM transfer to other microorganism species spread in the areas of potential getting of the GMM into the environment (soil). The study revealed no negative effects of the GMM on the animal organism and the environment, including remote aftereffects.
authors.forename: E A
authors.initials: EA
authors.lastname: Stavskiĭ
authors.forename: A I
authors.initials: AI
authors.lastname: Leliak
authors.lastname: Larina N M
authors.forename: O N
authors.initials: ON
authors.lastname: Grishaeva
authors.forename: V V
authors.initials: VV
authors.lastname: Serebrov
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Gorbunov
authors.forename: L G
authors.initials: LG
authors.lastname: Duben'
authors.forename: L A
authors.initials: LA
authors.lastname: Timukina
authors.forename: L V
authors.initials: LV
authors.lastname: Mit'ko
authors.forename: R S
authors.initials: RS
authors.lastname: Koval'
authors.forename: V P
authors.initials: VP
authors.lastname: Khodyrev
mesh_terms: D000818:Animals; D001412:Bacillus subtilis / Q000235:genetics* / Q000254:growth & development* / Q000302:isolation & purification; D002417:Cattle / Q000382:microbiology*; D004777:Environment; D041981:Gastrointestinal Tract / Q000382:microbiology; D022761:Gene Transfer, Horizontal; D005818:Genetic Engineering; D006801:Humans; D016898:Interferon-alpha / Q000235:genetics*; D012306:Risk
chemical_list: D016898:Interferon-alpha