title: Flow and pressure during liver preservation under ex situ and in situ perfusion with University of Wisconsin solution and histidine-tryptophan-ketoglutarate solution.
abstract: Effective preservation of liver grafts is the first essential step for successful liver transplantation. Insufficient perfusion leads to ischemic-type biliary lesions after transplantation. Perfusion of the graft can be performed either in situ or ex situ, with gravity flow or pressure-controlled. Mainly University of Wisconsin (UW) and histidine-tryptophan-ketoglutarate (HTK) solutions are used widespread in clinical liver transplantation. Due to a persistent lack of data, we performed this systematic investigation of in situ and ex situ perfusion of liver grafts with HTK (low-viscous) and UW (high-viscous) solutions at different pressure steps on the perfusion solution (gravity flow, 50, 100, 150, and 200 mm Hg). End points were perfusion flow and pressure in the hepatic artery. A pig model was used with n = 8 pigs randomized to each (HTK and UW) group. In situ perfusion was ineffective for both solutions at any pressure on the perfusate bag. Ex situ perfusion showed significantly improved flow and pressure in the hepatic artery and, therefore, was highly effective. No major differences between HTK and UW solutions could be detected. Therefore, an additional ex situ perfusion of the hepatic artery should be mandatory in every liver procurement.
authors.affiliation: Department of Transplantation and Hepatobiliarypancreatic Surgery, Johannes Gutenberg University, Mainz, Germany. christian.moench@planet-interkom.de
authors.forename: C
authors.initials: C
authors.lastname: Moench
authors.forename: A
authors.initials: A
authors.lastname: Heimann
authors.forename: D
authors.initials: D
authors.lastname: Foltys
authors.forename: B
authors.initials: B
authors.lastname: Schneider
authors.forename: S
authors.initials: S
authors.lastname: Minouchehr
authors.forename: E
authors.initials: E
authors.lastname: Schwandt
authors.forename: M
authors.initials: M
authors.lastname: Knaak
authors.forename: O
authors.initials: O
authors.lastname: Kempski
authors.forename: G
authors.initials: G
authors.lastname: Otto
mesh_terms: D000241:Adenosine / Q000008:administration & dosage; D000493:Allopurinol / Q000008:administration & dosage; D000818:Animals; D005947:Glucose / Q000008:administration & dosage; D005978:Glutathione / Q000008:administration & dosage; D006499:Hepatic Artery; D007328:Insulin / Q000008:administration & dosage; D008099:Liver / Q000187:drug effects / Q000601:surgery*; D008297:Male; D008353:Mannitol / Q000008:administration & dosage; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions / Q000008:administration & dosage*; D011189:Potassium Chloride / Q000008:administration & dosage; D011312:Pressure; D011343:Procaine / Q000008:administration & dosage; D011887:Raffinose / Q000008:administration & dosage; D012212:Rheology; D013552:Swine; D020858:Tissue and Organ Harvesting / Q000379:methods*
chemical_list: C039984:Bretschneider cardioplegic solution; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D008353:Mannitol; D011343:Procaine; D000493:Allopurinol; D011189:Potassium Chloride; D005978:Glutathione; D005947:Glucose; D000241:Adenosine; D011887:Raffinose