title: Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis.
abstract: Xylose is a major constituent of plant lignocellulose, and its fermentation is important for the bioconversion of plant biomass to fuels and chemicals. Pichia stipitis is a well-studied, native xylose-fermenting yeast. The mechanism and regulation of xylose metabolism in P. stipitis have been characterized and genes from P. stipitis have been used to engineer xylose metabolism in Saccharomyces cerevisiae. We have sequenced and assembled the complete genome of P. stipitis. The sequence data have revealed unusual aspects of genome organization, numerous genes for bioconversion, a preliminary insight into regulation of central metabolic pathways and several examples of colocalized genes with related functions. The genome sequence provides insight into how P. stipitis regulates its redox balance while very efficiently fermenting xylose under microaerobic conditions.
authors.affiliation: US Department of Agriculture, Forest Service, Forest Products Laboratory, One Gifford Pinchot Drive, Madison, Wisconsin 53705, USA.
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Jeffries
authors.forename: Igor V
authors.initials: IV
authors.lastname: Grigoriev
authors.forename: Jane
authors.initials: J
authors.lastname: Grimwood
authors.forename: José M
authors.initials: JM
authors.lastname: Laplaza
authors.forename: Andrea
authors.initials: A
authors.lastname: Aerts
authors.forename: Asaf
authors.initials: A
authors.lastname: Salamov
authors.forename: Jeremy
authors.initials: J
authors.lastname: Schmutz
authors.forename: Erika
authors.initials: E
authors.lastname: Lindquist
authors.forename: Paramvir
authors.initials: P
authors.lastname: Dehal
authors.forename: Harris
authors.initials: H
authors.lastname: Shapiro
authors.forename: Yong-Su
authors.initials: YS
authors.lastname: Jin
authors.forename: Volkmar
authors.initials: V
authors.lastname: Passoth
authors.forename: Paul M
authors.initials: PM
authors.lastname: Richardson
mesh_terms: D018533:Biomass; D053898:Biosynthetic Pathways / Q000235:genetics*; D002482:Cellulose / Q000378:metabolism*; D004271:DNA, Fungal / Q000032:analysis; D005285:Fermentation; D015723:Gene Library; D016680:Genome, Bacterial / Q000235:genetics*; D008031:Lignin / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010843:Pichia / Q000201:enzymology / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014994:Xylose / Q000378:metabolism*
chemical_list: D004271:DNA, Fungal; C036909:lignocellulose; D002482:Cellulose; D008031:Lignin; D014994:Xylose