Differences in cell morphology, lipid and apo B secretory capacity in caco-2 cells following long term treatment with saturated and monounsaturated fatty acids.
The suitability of the caco-2 cell line as a model for studying the long term impact of dietary fatty acids on intestinal lipid handling and chylomicron production was examined. Chronic supplementation of caco-2 cells with palmitic acid (PA) resulted in a lower triacylglycerol secretion than oleic acid (OA). This was coupled with a detrimental effect of PA, but not OA, on transepithelial electrical resistance (TER) measurements, suggesting a loss of structural integrity across the cell monolayer. Addition of OA reversed the adverse effects of PA and stearic acid on TER and increased the ability of cells to synthesise and accumulate lipid, but did not normalise the secretion of lipids by caco-2 cells. Increasing amounts of OA and decreasing amounts of PA in the incubation media markedly improved the ability of cells to synthesise apolipoprotein B and secrete lipids. Real time RT-PCR revealed a down regulation of genes involved in lipoprotein synthesis following PA than OA. Electron microscopy showed adverse effects of PA on cellular morphology consistent with immature enterocytes such as stunted microvilli and poor tight junction formation. In conclusion, previously reported differences in lipoprotein secretion by caco-2 cells supplemented with saturated fatty acids (SFA) and OA may partly reflect early cytotoxic effects of SFA on cellular integrity and function.
Bateman
Paul A
PA
Hugh Sinclair Unit of Human Nutrition, Department of Food Biosciences, School of Chemistry, Food Biosciences and Pharmacy, The University of Reading, Reading Berkshire, UK.
Jackson
Kim G
KG
Maitin
Vatsala
V
Yaqoob
Parveen
P
Williams
Christine M
CM
D053299:Apolipoprotein B-100 / Q000378:metabolism; D053283:Apolipoprotein B-48 / Q000378:metabolism; D001055:Apolipoproteins B / Q000096:biosynthesis / Q000378:metabolism*; D018938:Caco-2 Cells; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D015536:Down-Regulation / Q000187:drug effects; D017097:Electric Impedance; D020895:Enterocytes / Q000187:drug effects* / Q000473:pathology* / Q000648:ultrastructure; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009829:Oleic Acids / Q000494:pharmacology*; D019308:Palmitic Acid / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D019108:Tight Junctions / Q000187:drug effects / Q000648:ultrastructure; D013997:Time Factors; D014280:Triglycerides / Q000378:metabolism
D053299:Apolipoprotein B-100; D053283:Apolipoprotein B-48; D001055:Apolipoproteins B; D009829:Oleic Acids; D012333:RNA, Messenger; D014280:Triglycerides; D019308:Palmitic Acid; D007770:L-Lactate Dehydrogenase