[Preparation and characterization of the monoclonal antibody against human soluble mesothelin-related proteins].
OBJECTIVE
To prepare the monoclonal antibody (mAb) against human soluble mesothelin-related proteins (SMR) and identify its properties.


METHODS
The B-cell epitopes of mesothelin (MSLN) were predicted by multi-parameter prediction method. Then BALB/c mice were immunized with compound MSLN polypeptide prepare the mAb by hybridoma technique. The specificity of antibody was evaluated by immuocytochemistry and Western blot respectively.


RESULTS
Using the multi-parameter prediction of B-cell epitopes, the 471-481 epitope domain was selected and synthesized. Then the mice were immunized and mAb against human SMR was prepared and designated 2H10. The specificity of mAb 2H10 was evaluated to be high by using immuocytochemistry and Western blot.


CONCLUSIONS
The successful preparation of the mAb against SMR will provide efficient reagent for further detection of SMR by ELISA.
Chen
Shu-Cheng
SC
Department of Obstetrics and Gynaecology, the Fourth Hosipital of Hebei Medical University, Shijiazhuang, China.
Liu
Ya-Kun
YK
Wu
Xiao-Hua
XH
Shan
Bao-En
BE
Chen
Xing
X
Sun
Dong-Xia
DX
Wang
Li
L
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D015153:Blotting, Western; D058851:GPI-Linked Proteins; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007150:Immunohistochemistry; D008562:Membrane Glycoproteins / Q000276:immunology* / Q000378:metabolism; D000090204:Mesothelin; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D058851:GPI-Linked Proteins; C000715527:MSLN protein, human; D008562:Membrane Glycoproteins; C000715528:Msln protein, mouse; D000090204:Mesothelin