[The relationship of IL-1beta expressed in buccal cells and the polymorphisms of IL-1beta (+3953) with chronic periodontitis].
OBJECTIVE
The purpose of this study was to investigate the relationship of IL-1beta expressed in buccal cells and the polymorphisms of IL-1beta(+3953) with chronic periodontitis.


METHODS
Thirty-six patients with different severity of periodontal disease and thirty-six subjects with healthy periodontal tissues participated in this study. Buccal cells and peripheral venous blood samples were collected and the periodontal status were recorded. Immunohistochemical analysis of IL-1beta expressed in buccal cells was carried out and the polymorphisms in IL-1beta(+3953) were analyzed by polymerase chain reaction-restriction fragment length polymorphism after extracting DNA from peripheral venous blood. Then the differences in the level of IL-1beta expressed in buccal cells and the distribution of each genotype in IL-1beta(+3953) were compared. Multiple linear regression was used to correlate the periodontal status with the polymorphisms in IL-1beta(+3953) and the expression of IL-1beta) in buccal cells.


RESULTS
The carrying ratio of IL-1beta(+3953) allel II in severe chronic periodontitis (12%) was significantly higher than the healthy control group(0). The level of IL-1beta expressed in buccal cells of patients with chronic periodontitis was also significantly higher than that of subjects with healthy periodontal conditions (chi2 = 365.095, P < 0.001). Clinical attachment loss and probing depth correlated positively with the expression of IL-1beta (CAL, P < 0.05; PD, P < 0.01) and no correlation was found between plaque index, bleeding on probing,the polymorphisms in IL-1beta(+3959) and the levels of IL-1beta.


CONCLUSIONS
The results of this small sample study suggested that the level of IL-1beta expressed in buccal cells showed the severity of chronic periodontitis and the IL-1beta(+3953) genotype did not make the level of IL-1beta expressed in buccal cells changed significantly.
Tian
Ya-Guang
YG
Department of Periodontology, College of Stomatology, China Medical University, Shenyang 110002, Liaoning Province, China. yap_nancy@yahoo.com
Pan
Ya-Ping
YP
Lin
Li
L
Zhang
Dong-Mei
DM
Zhao
Jian
J
D055113:Chronic Periodontitis / Q000235:genetics*; D003774:Dental Plaque Index; D005260:Female; D005838:Genotype; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics* / Q000378:metabolism*; D009061:Mouth Mucosa; D017622:Periodontal Attachment Loss; D010512:Periodontal Index; D010519:Periodontium; D012150:Polymorphism, Restriction Fragment Length
D053583:Interleukin-1beta