Characterization of the NikA histidine kinase implicated in the phosphorelay signal transduction of Aspergillus nidulans, with special reference to fungicide responses.
We recently compiled a complete list of phosphorelay signal transduction components in the model filamentous fungus Aspergillus nidulans. In this study, we characterized a histidine protein kinase (designated NikA) that is found in many fungi, with special reference to responses to potent fungicides (iprodione and fludioxonil). We provided evidence that not only NikA, but also two downstream response regulators (SskA and SrrA) are crucially implicated in the mode of action of these fungicides, and also that the further downstream HogA-MAPK cascade is exaggerated abnormally (or ectopically) in hyphae by the fungicides in a manner dependent on the NikA-SskA phosphorelay.
Hagiwara
Daisuke
D
Laboratory of Molecular Microbiology, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan. t2706693@mbox.nagoya-u.ac.jp
Matsubayashi
Yoshihiro
Y
Marui
Junichiro
J
Furukawa
Kentaro
K
Yamashino
Takafumi
T
Kanamaru
Kyoko
K
Kato
Masashi
M
Abe
Keietsu
K
Kobayashi
Tetsuo
T
Mizuno
Takeshi
T
D000620:Aminoimidazole Carboxamide / Q000031:analogs & derivatives* / Q000494:pharmacology; D001233:Aspergillus nidulans / Q000502:physiology*; D004149:Dioxoles / Q000494:pharmacology*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D005659:Fungicides, Industrial / Q000494:pharmacology*; D000071677:Histidine Kinase; D006827:Hydantoins / Q000494:pharmacology*; D009154:Mutation; D010766:Phosphorylation; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D011758:Pyrroles / Q000494:pharmacology*; D015398:Signal Transduction / Q000502:physiology
D004149:Dioxoles; D005656:Fungal Proteins; D005659:Fungicides, Industrial; D006827:Hydantoins; D011758:Pyrroles; D000620:Aminoimidazole Carboxamide; D011494:Protein Kinases; D000071677:Histidine Kinase; C108339:fludioxonil; C033148:iprodione