The Drosophila SET domain encoding gene dEset is essential for proper development.
We have identified dEset, the fly homolog of human SETDB1 and mouse ESET histone lysine methyltransferases (HKMTases) that methylates the lysine 9 residue of histone 3 (H3-K9) and negatively regulates transcription of target genes. By using spatio-temporal RNA interference we show that dEset is required at several stages of development coinciding with ecdysone pulses, possibly as a repressor of transcription of target genes. Several interacting partners, for example USP, spire, and cut up were identified in a yeast two-hybrid screen. The spatio-temporal expression profiles of dEset and its potential partners suggest that they may act together or even in a larger complex.
Stabell
Marianne
M
Institute of Molecular Biosciences, University of Oslo, P.O.B. 1041 Blindern, NO-0316 Oslo, Norway.
Bjørkmo
Mona
M
Aalen
Reidunn B
RB
Lambertsson
Andrew
A
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D019521:Body Patterning / Q000235:genetics; D004330:Drosophila / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D029721:Drosophila Proteins / Q000235:genetics / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D011495:Histone-Lysine N-Methyltransferase / Q000235:genetics / Q000502:physiology*; D008297:Male; D051379:Mice; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010641:Phenotype; D011496:Protein Methyltransferases / Q000235:genetics; D017434:Protein Structure, Tertiary; D034622:RNA Interference; D020798:Two-Hybrid System Techniques
D029721:Drosophila Proteins; D011496:Protein Methyltransferases; D011495:Histone-Lysine N-Methyltransferase; C516239:SETDB1 protein, mouse; C519551:egg protein, Drosophila