title: Ex vivo generation of mature and functional human smooth muscle cells differentiated from skeletal myoblasts.
abstract: We described the ex vivo production of mature and functional human smooth muscle cells (SMCs) derived from skeletal myoblasts. Initially, myoblasts expressed all myogenic cell-related markers such as Myf5, MyoD and Myogenin and differentiate into myotubes. After culture in a medium containing vascular endothelial growth factor (VEGF), these cells were shown to have adopted a differentiated SMC identity as demonstrated by alphaSMA, SM22alpha, calponin and smooth muscle-myosin heavy chain expression. Moreover, the cells cultured in the presence of VEGF did not express MyoD anymore and were unable to fuse in multinucleated myotubes. We demonstrated that myoblasts-derived SMCs (MDSMCs) interacted with endothelial cells to form, in vitro, a capillary-like network in three-dimensional collagen culture and, in vivo, a functional vascular structure in a Matrigel implant in nonobese diabetic-severe combined immunodeficient mice. Based on the easily available tissue source and their differentiation into functional SMCs, these data argue that skeletal myoblasts might represent an important tool for SMCs-based cell therapy.
authors.affiliation: Institut des Vaisseaux et du Sang et Centre de Recherche Cardiovasculaire, INSERM U689, Paris, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Le Ricousse-Roussanne
authors.forename: Jerome
authors.initials: J
authors.lastname: Larghero
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Zini
authors.forename: Veronique
authors.initials: V
authors.lastname: Barateau
authors.forename: Philippe
authors.initials: P
authors.lastname: Foubert
authors.forename: Georges
authors.initials: G
authors.lastname: Uzan
authors.forename: Xuhui
authors.initials: X
authors.lastname: Liu
authors.forename: Marie-Noelle
authors.initials: MN
authors.lastname: Lacassagne
authors.forename: Brigitte
authors.initials: B
authors.lastname: Ternaux
authors.forename: Isabelle
authors.initials: I
authors.lastname: Robert
authors.forename: Marc
authors.initials: M
authors.lastname: Benbunan
authors.forename: Jean-Thomas
authors.initials: JT
authors.lastname: Vilquin
authors.forename: Karine
authors.initials: K
authors.lastname: Vauchez
authors.forename: Gerard
authors.initials: G
authors.lastname: Tobelem
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Marolleau
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002454:Cell Differentiation; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology*; D004338:Drug Combinations; D004847:Epithelial Cells / Q000502:physiology; D005260:Female; D006801:Humans; D007797:Laminin / Q000494:pharmacology*; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D018485:Muscle Fibers, Skeletal / Q000502:physiology; D032448:Myoblasts, Skeletal / Q000187:drug effects / Q000378:metabolism / Q000502:physiology* / Q000637:transplantation; D032389:Myocytes, Smooth Muscle / Q000378:metabolism / Q000502:physiology* / Q000637:transplantation; D018919:Neovascularization, Physiologic; D011509:Proteoglycans / Q000494:pharmacology*; D046509:Tissue Culture Techniques; D014183:Transplantation, Heterologous; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology
chemical_list: D015415:Biomarkers; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; D042461:Vascular Endothelial Growth Factor A; C059485:matrigel; D003094:Collagen