Detection of amyloid-beta oligomers in human cerebrospinal fluid by flow cytometry and fluorescence resonance energy transfer.
The neuropathology of Alzheimer's disease (AD) has been linked recently to non-fibrillar forms of neurotoxic amyloid-beta (Abeta) oligomers of which high levels are observed in the brain of AD patients. This suggests that Abeta oligomers play a key role in the early events of AD, underlining their potential for the early diagnosis of the disease. We have developed an extremely sensitive assay for the detection of oligomeric and fibrillar structures of Abeta that is based on multiparametric analysis of data obtained by flow cytometry and fluorescence resonance energy transfer (Fret). The assay readily detects Abeta oligomers in human cerebrospinal fluid (CSF) as verified by dot blot of the isolated particles. By measuring 174 CSF samples of non-demented control patients with various neurological disorders a high reliability and reproducibility of the method could be demonstrated.
Santos
Alexander Navarrete
AN
ACGT ProGenomics AG, Halle, Germany. alexander.navarrete@medizin.uni-halle.de
Torkler
Sandra
S
Nowak
Denny
D
Schlittig
Claudia
C
Goerdes
Mirjam
M
Lauber
Thomas
T
Trischmann
Lothar
L
Schaupp
Michael
M
Penz
Monika
M
Tiller
Friedrich-Wilhelm
FW
Böhm
Gerald
G
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000134:cerebrospinal fluid* / Q000175:diagnosis*; D016229:Amyloid beta-Peptides / Q000134:cerebrospinal fluid*; D002493:Central Nervous System Diseases / Q000134:cerebrospinal fluid / Q000175:diagnosis; D002648:Child; D042241:Early Diagnosis; D005260:Female; D005434:Flow Cytometry; D031541:Fluorescence Resonance Energy Transfer; D005625:Frontal Lobe / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D009838:Oligodeoxyribonucleotides / Q000134:cerebrospinal fluid*; D011237:Predictive Value of Tests; D013223:Statistics as Topic; D013702:Temporal Lobe / Q000378:metabolism
D016229:Amyloid beta-Peptides; D009838:Oligodeoxyribonucleotides