title: [Clinical utility of in situ hybridization for diagnosing respiratory tract infection and sepsis].
abstract: We studied the clinical utility of in situ hybridization (Hybrisep) in diagnosing respiratory infection and/ or sepsis. Peripheral blood was taken from patients with respiratory infections and suspected sepsis for both routine blood culture and in situ hybridization, and focal samples including sputum, bronchoalveolar lavage, and central and thoracic catheter, were simultaneously examined for bacterial culture. Specimens numbered 46. The clinical diagnosis was 20 cases of septicemia, with 26 specimens diagnosed as respiratory infectious diseases including hospital-acquired pneumonia and pleurisy. Positive cases of in situ hybridization were seen in 19 specimens (41.3%) in all specimens, significantly higher than that in blood culture (17.4%). Respiratory infection showed a high positive rate in in situ hybridization. Interestingly, the bacterial pathogen detected by in situ hybridization was not always consistent with that taken from focal samples. Whether the pathogen isolated by in situ hybridization is accurate etiologically or diagnostically remains unknown, but our findings suggest a polymicrobial infection in patients with hospital-acquired respiratory infections. In situ hybridization thus provides important information on the etiological pathogen in different infectious diseases.
authors.affiliation: First Department of Medicine, Shinshu University.
authors.forename: Mikako
authors.initials: M
authors.lastname: Tsunoda
authors.forename: Tomonobu
authors.initials: T
authors.lastname: Koizumi
authors.forename: Masanori
authors.initials: M
authors.lastname: Yasuo
authors.forename: Kenji
authors.initials: K
authors.lastname: Tsushima
authors.forename: Masayuki
authors.initials: M
authors.lastname: Hanaoka
authors.forename: Keishi
authors.initials: K
authors.lastname: Kubo
authors.forename: Takayuki
authors.initials: T
authors.lastname: Honda
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Yamazaki
authors.forename: Shigeru
authors.initials: S
authors.lastname: Koyama
authors.forename: Masahiro
authors.initials: M
authors.lastname: Tsukadaira
authors.forename: Kazue
authors.initials: K
authors.lastname: Tuge
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Tamai
authors.forename: Eiji
authors.initials: E
authors.lastname: Yanagisawa
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018893:Bronchoalveolar Lavage; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006801:Humans; D017403:In Situ Hybridization / Q000295:instrumentation; D007962:Leukocytes / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000382:microbiology; D018805:Sepsis / Q000175:diagnosis* / Q000382:microbiology; D013183:Sputum / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic