title: Orientation Determination of Membrane-Disruptive Proteins Using Powder Samples and Rotational Diffusion: A Simple Solid-State NMR Approach.
abstract: The orientation of membrane proteins undergoing fast uniaxial rotation around the bilayer normal can be determined without macroscopic alignment. We show that the motionally averaged powder spectra exhibit their 0° frequency, [Formula: see text], at the same position as the peak of an aligned sample with the alignment axis parallel to the magnetic field. This equivalence is exploited to determine the orientation of a β-sheet antimicrobial peptide not amenable to macroscopic alignment, using (13)CO and (15)N chemical shifts from powder spectra. This powder sample approach permits orientation determination of naturally membrane-disruptive proteins in diverse environments and under magic-angle spinning.
authors.affiliation: Department of Chemistry, Iowa State University, Ames, IA 50011.
authors.forename: Mei
authors.initials: M
authors.lastname: Hong
authors.forename: Tim
authors.initials: T
authors.lastname: Doherty