[Establishment of an animal model of azoospermia in male mice].
OBJECTIVE
To establish a stable animal model of azoospermia in male mice.


METHODS
Two groups of mice, with 30 in each, were intervened respectively by chemotherapy (intraperitoneal injection of Busulfan and Cyclophosphamide) and subcutaneous injection of Estradiol. At different times after the injection, the microscopic structures of the seminiferous tubules of both groups were observed and compared. The recovery of the germ cells in the seminiferous tubules was also evaluated after the termination of the intervention.


RESULTS
A stable animal model was established by chemotherapeutic method with Busulfan and cyclophosphamide, while the model constructed by muscle injection of Estradiol was not stable.


CONCLUSIONS
Compared with estrogen injection, chemotherapeutic intervention is a reliable method for constructing an animal model of azoospermia in male mice.
Huang
Xun-bin
XB
Center for Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China. huangxb@mails.tjmu.edu.cn
Li
Hong-gang
HG
D000818:Animals; D053713:Azoospermia; D002066:Busulfan / Q000008:administration & dosage; D003520:Cyclophosphamide / Q000008:administration & dosage; D004195:Disease Models, Animal; D004958:Estradiol / Q000008:administration & dosage; D007274:Injections, Intraperitoneal; D007279:Injections, Subcutaneous; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL
D004958:Estradiol; D003520:Cyclophosphamide; D002066:Busulfan