[Molecular typing of Salmonella paratyphi A isolates from four provinces with pulse-field gel electrophoresis].
OBJECTIVE
To analyze the molecular types of Salmonella paratyphi A strains isolated in the recent years, and to construct the standard S. paratyphi A databank in the laboratory surveillance network PulseNet China.


METHODS
S. paratyphi A isolates from 4 provinces were analyzed with the standard pulsed-field gel electrophoresis (PFGE) protocol used in PulseNet and their patterns compared. The databank was constructed with BioNumerics.


RESULTS
Eleven PFGE patterns were obtained, in which 3 predominant patterns were identifies with a similarity coefficient of 96.3%. The strains of these patterns, accounted for 86.5% of the analyzed strains, appeared in different provinces and years.


CONCLUSIONS
The databank of S. paratyphi A was constructed and could be used in laboratory surveillance of S. paratyphi A in PulseNet China. From the analyses on molecular typing of the isolates, data suggested that the predominant strains might cause the epidemics in different regions.
Li
Wei
W
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Cui
Zhi-gang
ZG
Zhang
Zheng
Z
Tian
Ke-cheng
KC
Zhong
Hao-jie
HJ
Zhu
Feng-cai
FC
Liang
Wei-li
WL
Zhang
Li-juan
LJ
Kan
Biao
B
D002681:China; D016521:Electrophoresis, Gel, Pulsed-Field; D012482:Salmonella paratyphi A / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification