title: Correlation between optical coherence tomography images and histology of pigskin.
abstract: Noninvasive imaging techniques such as optical coherence tomography (OCT) are being widely used for early diagnostics of a variety of pathologies. Traditional tissue preparation for histological evaluation alters the dimensions of histological images such that differences between the dimensions of histologic and OCT images can be as great as 2.5-fold. This discrepancy complicates quantitative interpretation of OCT images. Here we used a steel ring sewn to the pigskin to avoid tissue deformation due to excision and fixation and used a surgical suture as a fixed tissue marker. With these techniques, we achieved good correlation between OCT and histological images with differences of less than 10%. The resulting method significantly enhances the diagnostic capabilities of the OCT technique.
authors.affiliation: University of Texas Medical Branch, Galveston, Texas 77555-0456, USA.
authors.forename: Roman
authors.initials: R
authors.lastname: Kuranov
authors.forename: Veronika
authors.initials: V
authors.lastname: Sapozhnikova
authors.forename: Donald
authors.initials: D
authors.lastname: Prough
authors.forename: Inga
authors.initials: I
authors.lastname: Cicenaite
authors.forename: Rinat
authors.initials: R
authors.lastname: Esenaliev
mesh_terms: D000818:Animals; D006652:Histological Techniques / Q000379:methods*; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012867:Skin / Q000166:cytology*; D013223:Statistics as Topic; D013552:Swine; D041623:Tomography, Optical Coherence / Q000379:methods*