Adaptation of a photochemical method to initiate recurrent platelet-mediated thrombosis in small animals.
Platelet-mediated thrombosis represents the initial precipitating event in the genesis of unstable angina, acute myocardial infarction, and stroke. As a result, there is considerable interest in the preclinical discovery and screening of new 'anti-platelet' therapies aimed at limiting the incidence and reoccurrence of arterial thrombosis-efforts that, to date, have largely required the use of large animal models of thrombotic occlusion. In the current report, we describe the successful development of a small-animal (rat) model of spontaneous and recurrent platelet-mediated arterial thrombosis achieved by the in vivo administration of a photoactive dye (rose bengal) followed by focal illumination with green laser light.
Przyklenk
Karin
K
Department of Emergency Medicine, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA. Karin.Przyklenk@umassmed.edu
Whittaker
Peter
P
D000818:Animals; D004195:Disease Models, Animal; D005263:Femoral Artery / Q000503:physiopathology / Q000528:radiation effects*; D007834:Lasers; D010777:Photochemistry; D010974:Platelet Aggregation / Q000502:physiology; D051381:Rats; D012008:Recurrence; D012395:Rose Bengal / Q000494:pharmacology*; D013927:Thrombosis / Q000209:etiology* / Q000503:physiopathology
D012395:Rose Bengal