Dose-response studies for the elicitation of CD8 T cells by a DNA vaccine, used alone or as the prime for a modified vaccinia Ankara boost.
Here, we conduct dose-response studies in mice for the elicitation of CD8 T cells by a DNA vaccine that expresses HIV Gag. For DNA doses ranging from 1 to 100 microg, the studies revealed greater than 10-fold increases in anti-Gag CD8 T cells following a DNA prime or a DNA prime and a constant modified vaccinia Ankara (MVA) boost. These results are in contrast to dose-response studies for MVA vectors expressing Gag, where only 2-3-fold increases in anti-Gag CD8 T cells were elicited by 100-fold increases in dose.
Liu
Jinyan
J
Yerkes National Primate Research Center and Emory Vaccine Center of Emory University, Atlanta, GA, United States.
Hellerstein
Michael
M
McDonnel
Michael
M
Amara
Rama Rao
RR
Wyatt
Linda S
LS
Moss
Bernard
B
Robinson
Harriet L
HL
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D016655:HIV Core Protein p24 / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D014616:Vaccinia virus / Q000276:immunology*
D016655:HIV Core Protein p24; D019444:Vaccines, DNA