Active tissue-specific DNA demethylation conferred by somatic cell nuclei in stable heterokaryons.
DNA methylation is among the most stable epigenetic marks, ensuring tissue-specific gene expression in a heritable manner throughout development. Here we report that differentiated mesodermal somatic cells can confer tissue-specific changes in DNA methylation on epidermal progenitor cells after fusion in stable multinucleate heterokaryons. Myogenic factors alter regulatory regions of genes in keratinocyte cell nuclei, demethylating and activating a muscle-specific gene and methylating and silencing a keratinocyte-specific gene. Because these changes occur in the absence of DNA replication or cell division, they are mediated by an active mechanism. Thus, the capacity to transfer epigenetic changes to other nuclei is not limited to embryonic stem cells and oocytes but is also a property of highly specialized mammalian somatic cells. These results suggest the possibility of directing the reprogramming of readily available postnatal human progenitor cells toward specific tissue cell types.
Zhang
Fan
F
Baxter Laboratory in Genetic Pharmacology, Departments of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305-3175, USA.
Pomerantz
Jason H
JH
Sen
George
G
Palermo
Adam T
AT
Blau
Helen M
HM
D000818:Animals; D002455:Cell Division; D002467:Cell Nucleus / Q000378:metabolism*; D018899:CpG Islands; D004247:DNA / Q000378:metabolism; D019175:DNA Methylation; D053595:Embryonic Stem Cells / Q000166:cytology; D000078404:Epidermal Cells; D044127:Epigenesis, Genetic; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D008856:Microscopy, Fluorescence; D053652:Nuclear Transfer Techniques; D013234:Stem Cells / Q000378:metabolism
D004247:DNA