Genetic polymorphisms influence Plasmodium ovale PCR detection accuracy.
Detection of Plasmodium ovale by use of a nested PCR assay with a novel Plasmodium ovale primer set was superior to detection of Plasmodium ovale by real-time PCR assays. Nested PCR was also better at detecting P. malariae. The detection of P. ovale in many patients first admitted >2 months following their return to Italy indicated that P. ovale relapses are common.
Calderaro
A
A
Faculty of Medicine and Surgery, University of Parma, Department of Pathology and Laboratory Medicine, Section of Microbiology, Viale A. Gramsci, 14-43100 Parma, Italy. adriana.calderaro@unipr.it
Piccolo
G
G
Perandin
F
F
Gorrini
C
C
Peruzzi
S
S
Zuelli
C
C
Ricci
L
L
Manca
N
N
Dettori
G
G
Chezzi
C
C
Snounou
G
G
D000328:Adult; D000818:Animals; D002648:Child; D002675:Child, Preschool; D006801:Humans; D008288:Malaria / Q000175:diagnosis* / Q000469:parasitology*; D008875:Middle Aged; D041122:Plasmodium ovale / Q000235:genetics* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D016053:RNA, Protozoan / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D016053:RNA, Protozoan; D012335:RNA, Ribosomal