title: [Study of cryopreservation of differentiated hepatocyte derived from human bone marrow stem cells].
abstract: OBJECTIVE
  To observe the viability and function of human bone marrow stem cell-derived hepatocytes following cryopreservation in vitro.
  
  
  METHODS
  Human bone marrow cells were induced to differentiate into hepatocytes in the presence of multiple factors. Mature hepatocytes were cryopreserved in 90% FBS and 10% DMSO (Group A), 10% FBS, 30% glycerol and 60% conditioned medium (Group B), and 10% FBS, 10% DMSO, and 80% UW solution (Group C). The cells were thawed after 4 weeks, and the cell viability and the albumin level were determined.
  
  
  RESULTS
  The human bone marrow derived hepatocytes maintained functional morphology after thawing. The viabilities in Group A, B and C were (60.0 +/- 3.3)%, (91.0 +/- 2.6)%, and (89.0 +/- 1.4)%, respectively. After culturing for 24 h, the albumin levels in Group A, B and C were (0.210 +/- 0.005) g/L, (0.340 +/- 0.020) g/L and (0.330 +/- 0.030) g/L, respectively.
  
  
  CONCLUSIONS
  Human bone marrow stem cell-derived hepatocytes can maintain the viability and function after cryopreservation. These cells may contribute to an important source of hepatocytes for clinical hepatocyte transplantation and artificial liver support system.
authors.affiliation: Department of General Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Qu
authors.forename: Bing-Lu
authors.initials: BL
authors.lastname: Li
authors.forename: Yu-Pei
authors.initials: YP
authors.lastname: Zhao
authors.forename: Cui-Zhu
authors.initials: CZ
authors.lastname: Chen
authors.forename: Xu
authors.initials: X
authors.lastname: Yan
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: He
mesh_terms: D000328:Adult; D001854:Bone Marrow Cells / Q000166:cytology* / Q000502:physiology; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D022781:Hepatocytes / Q000166:cytology* / Q000502:physiology / Q000637:transplantation; D006801:Humans; D023461:Myeloid Progenitor Cells / Q000166:cytology* / Q000502:physiology
chemical_list: D003451:Cryoprotective Agents