title: The effect of green pit viper (Trimeresurus albolabris) venom on platelet morphology by electron microscopy.
abstract: The incidence of venomous snake bites increases every year in Thailand, especially due to green pit viper. After the bite, there is bleeding due to thrombin-like property of the venom. The mean platelet volume has been reported to be decreased in those who have been bitten by this snake. In this study we investigate the effect of green pit viper venom (Trimeresurus albolabris) on platelet volume (MPV), number and morphology of platelets in vitro. The test was carried out by washing platelets in phosphate buffer at pH 7.2 to remove fibrinogen, then the washed platelets were mixed with green pit viper venom. Platelet morphology was examined by scanning electron microscope (SEM). The morphology of platelets was smaller than normal which ranges from 1.1- 1.2 microm. Green pit viper venom can directly effect platelet morphology, decreasing platelet volume.
authors.affiliation: Clinical Microscopy Research Unit, Department of Clinical Microscopy, Faculty of Allied Health Sciences, Bangkok 10330, Thailand. suphan.s@chula.ac.th
authors.forename: Suphan
authors.initials: S
authors.lastname: Soogarun
authors.forename: Montri
authors.initials: M
authors.lastname: Choewbamrungkiat
authors.forename: Viroj
authors.initials: V
authors.lastname: Wiwanitkit
authors.forename: Jamsai
authors.initials: J
authors.lastname: Suwansaksri
authors.forename: Waykin
authors.initials: W
authors.lastname: Nopanitaya
authors.forename: Paweena
authors.initials: P
authors.lastname: Pradniwat
authors.forename: Attakorn
authors.initials: A
authors.lastname: Palasuwan
authors.forename: Supantitra
authors.initials: S
authors.lastname: Chanprasert
authors.forename: Panchalee
authors.initials: P
authors.lastname: Jangprasert
authors.forename: Pacharaporn
authors.initials: P
authors.lastname: Netsupun
authors.forename: Wacharin
authors.initials: W
authors.lastname: Sirisapsombat
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects*; D048429:Cell Size / Q000187:drug effects; D003435:Crotalid Venoms / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D010976:Platelet Count; D013785:Thailand; D017841:Trimeresurus
chemical_list: D003435:Crotalid Venoms