title: [Biodistribution study of 131I-gM-CSF in SCID mice bearing human leukemia].
abstract: OBJECTIVE
  To investigate the biodistribution of 131I-GM-CSF in SCID mice bearing human AML in vivo.
  
  
  METHODS
  The xenograft model of human leukemia was established in SCID mice. In the leukemia mice, the biodistribution of 131I-GM-CSF produced by chlo amine-T method was studied.
  
  
  RESULTS
  (1)The inoculated HL-60 cells could grow in SCID mice, which developed leukemia after 4 weeks. (2) 131 I-GM-CSF was concentrated in spleen, bone marrow and tumor tissue of the mice. In spleen and bone marrow, 131 I-GM-CSF was uptaken to peak in 30 minutes after injection, the up taking rate was (442. 9+/-86. 4) % ID/g and (4283. 8+/-252. 8)% ID/g, respectively, and maintained on higher level in 24 hours. The injection of 131I resulted in an even distribution in the whole body.
  
  
  CONCLUSIONS
  131 I-GM-CSF is able to concentrate electively in spleen, bone marrow and organs infiltrated by leukemia cells. The biodistribution of 131I-GM-CSF in the leukemia mice is tissue specific.
authors.affiliation: Department of Hematology, the Second College of Clinical Medicine, Chongqing Medical University, Chongqing 400010, China.
authors.forename: Yi-zhi
authors.initials: YZ
authors.lastname: Xu
authors.forename: Shi-feng
authors.initials: SF
authors.lastname: Lou
authors.forename: Yang-jia
authors.initials: YJ
authors.lastname: Deng
mesh_terms: D000818:Animals; D005260:Female; D005434:Flow Cytometry; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000493:pharmacokinetics*; D018922:HL-60 Cells; D006801:Humans; D007457:Iodine Radioisotopes; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism*; D051379:Mice; D016513:Mice, SCID; D023041:Xenograft Model Antitumor Assays
chemical_list: D007457:Iodine Radioisotopes; D016178:Granulocyte-Macrophage Colony-Stimulating Factor