title: Simultaneous co-immobilization of glucose oxidase and catalase in their substrates.
abstract: Glucose oxidase (GOD) and catalase (CAT) were simultaneously co-immobilized onto magnesium silicate (florisil) by covalent coupling. Glucose was added in immobilization mixture and hydrogen peroxide which is the substrate of CAT was produced in coupling mixture during immobilization time. Therefore, co-immobilization of GOD and CAT was carried out in presence of both their substrate: glucose and hydrogen peroxide, respectively. The effect of glucose concentration in immobilization mixture on activities of GOD and CAT of co-immobilized samples were investigated. Maximum GOD and CAT activities were determined for samples co-immobilized in presence of 15 and 20 mM glucose, respectively. Co-immobilization of GOD and CAT in presence of their substrates highly improved the activity and reusability of both enzymes.
authors.affiliation: University of Mustafa Kemal, Faculty of Arts and Sciences Department of Chemistry 31040 Hatay, Turkey. gozyilmaz@mku.edu.tr
authors.forename: G
authors.initials: G
authors.lastname: Ozyilmaz
authors.forename: S S
authors.initials: SS
authors.lastname: Tukel
mesh_terms: D002374:Catalase / Q000737:chemistry*; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D017633:Magnesium Silicates / Q000737:chemistry; D013379:Substrate Specificity
chemical_list: D004800:Enzymes, Immobilized; D017633:Magnesium Silicates; C005013:Florisil; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D002374:Catalase; D005947:Glucose