Ciliary band gene expression patterns in the embryo and trochophore larva of an indirectly developing polychaete.
The trochophore larvae of indirectly developing spiralians have ciliary bands with motor and feeding functions. The preoral prototroch ciliary band is the first differentiating organ in annelid and mollusk embryos. Here we report the expression of several ciliary band markers during embryogenesis and early larval stages of the indirectly developing polychaete Hydroides elegans. Genes with similarity to caveolin, beta-tubulin, alpha-tubulin, and tektin are expressed in the eight primary prototroch precursors, 1q(221) and 1q(212). Blastomeres 1q(221) and 1q(212) locate at the same equatorial latitude after the complementary asymmetric division of their 1q(22) and 1q(21) precursors. In addition, caveolin and alpha-tubulin are expressed in the metatroch and adoral ciliary zone. Caveolin is expressed in foregut ciliated cells, and alpha-tubulin is expressed in apical tuft ciliated cells. The expression of a beta-thymosin homolog is restricted to 1q(122) and 1q(121) blastomeres, which locate just above and in close association with the eight primary prototroch cells 1q(221) and 1q(212). In addition, the beta-thymosin homolog has a transient expression in the hindgut and apical zone. The expression of all these genes provides a landmark for the early specification of ciliary bands and other ciliated organs.
Arenas-Mena
Cesar
C
Department of Biology, San Diego State University, 5500 Campanile Drive, San Diego, CA 92182-4614, USA. arenas@science.sdsu.edu
Wong
Kimberly Suk-Ying
KS
Arandi-Forosani
Navid
N
D000818:Animals; D022461:Caveolins / Q000235:genetics / Q000378:metabolism; D002923:Cilia / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D007814:Larva / Q000166:cytology / Q000378:metabolism*; D008868:Microtubule Proteins / Q000235:genetics / Q000378:metabolism; D011077:Polychaeta / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D015347:RNA Probes; D013947:Thymosin / Q000031:analogs & derivatives / Q000235:genetics / Q000378:metabolism; D014404:Tubulin / Q000235:genetics / Q000378:metabolism; D014452:Ubiquitins / Q000235:genetics / Q000378:metabolism
D022461:Caveolins; D008868:Microtubule Proteins; D015347:RNA Probes; D014404:Tubulin; D014452:Ubiquitins; C044103:tektins; C047192:thymosin beta(1); D013947:Thymosin