Cutting Edge: Antibody-mediated TLR7-dependent recognition of viral RNA.
TLR7 recognizes the genome of ssRNA viruses such as Coxsackievirus B. Because TLR7 is expressed in intracellular compartments, viral RNA must be internalized before its recognition by TLR7. In this study, we define plasmacytoid dendritic cells (pDC) as peripheral blood mononuclear immune cells that respond to Coxsackievirus. pDC activation by Coxsackievirus B requires the presence of specific antiviral Abs. We show that Fc receptors mediate the recognition of virus-Ab complexes and that TLR7 is required for human and murine pDC production of cytokines. These data define a pathway by which intracellular TLR7 senses viral RNA and indicate a role for TLRs in association with Abs in sustaining virus-specific responses.
Wang
Jennifer P
JP
Department of Medicine, University of Massachusetts, 364 Plantation, Worcester, MA 01605, USA. jennifer.wang@umassmed.edu
Asher
Damon R
DR
Chan
Melvin
M
Kurt-Jones
Evelyn A
EA
Finberg
Robert W
RW
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D000936:Antigen-Antibody Complex / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D029822:Enterovirus B, Human / Q000276:immunology*; D006801:Humans; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D018345:Mice, Knockout; D010950:Plasma Cells / Q000276:immunology*; D012367:RNA, Viral / Q000276:immunology*; D011961:Receptors, Fc / Q000172:deficiency / Q000276:immunology; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology; D051199:Toll-Like Receptor 7 / Q000276:immunology*
D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex; D016207:Cytokines; D008562:Membrane Glycoproteins; D012367:RNA, Viral; D011961:Receptors, Fc; C477038:Tlr7 protein, mouse; D051199:Toll-Like Receptor 7