Rapid identification of Fusobacterium nucleatum and Fusobacterium necrophorum by fluorescence in situ hybridization.
Identification of clinically relevant Fusobacterium spp. is hampered by their slow growth, their frequent occurrence in polymicrobial culture, and the low reliability of biochemical differentiation methods. A newly developed fluorescence in situ hybridization (FISH) assay allowed reliable and rapid identification of Fusobacterium necrophorum and Fusobacterium nucleatum from culture. Preliminary results show that the method offers the perspective for direct detection of these pathogens in blood cultures and abscess aspirates.
Sigge
Anja
A
Department of Medical Microbiology and Hygiene, University Hospital of Ulm, D-89081 Ulm, Germany. anja.sigge@uniklinik-ulm.de
Essig
Andreas
A
Wirths
Beate
B
Fickweiler
Kristina
K
Kaestner
Nicole
N
Wellinghausen
Nele
N
Poppert
Sven
S
D005674:Fusobacterium Infections / Q000175:diagnosis* / Q000235:genetics; D005675:Fusobacterium necrophorum / Q000235:genetics / Q000302:isolation & purification*; D016967:Fusobacterium nucleatum / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D012680:Sensitivity and Specificity