Second acyl homoserine lactone production system in the extreme acidophile Acidithiobacillus ferrooxidans.
The acidophilic proteobacterium Acidithiobacillus ferrooxidans is involved in the industrial biorecovery of copper. It is found in acidic environments in biofilms and is important in the biogeochemical cycling of metals and nutrients. Its genome contains a cluster of four genes, glyQ, glysS, gph, and act, that are predicted to encode the alpha and beta subunits of glycine tRNA synthetase, a phosphatase, and an acyltransferase, respectively (GenBank accession no. DQ149607). act, cloned and expressed in Escherichia coli, produces acyl homoserine lactones (AHLs) principally of chain length C14 according to gas chromatography and mass spectrometry measurements. The AHLs have biological activity as shown by in vivo studies using the reporter strain Sinorhizobium meliloti Rm41 SinI-. Reverse transcription-PCR (RT-PCR) experiments indicate that the four genes are expressed as a single transcript, demonstrating that they constitute an operon. According to semiquantitative RT-PCR results, act is expressed more highly when A. ferrooxidans is grown in medium containing iron than when it is grown in medium containing sulfur. Since AHLs are important intercellular signaling molecules used by many bacteria to monitor their population density in quorum-sensing control of gene expression, this result suggests that A. ferrooxidans has two quorum-sensing systems, one based on Act, as described herein, and the other based on a Lux-like quorum-sensing system, reported previously. The latter system was shown to be upregulated in A. ferrooxidans grown in sulfur medium, suggesting that the two quorum-sensing systems respond to different environmental signals that may be related to their abilities to colonize and use different solid sulfur- and iron-containing minerals.
Rivas
Mariella
M
Institute of Cellular and Molecular Biology, Faculty of Medicine, University of Chile, Santiago, Chile.
Seeger
Michael
M
Jedlicki
Eugenia
E
Holmes
David S
DS
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000096:biosynthesis; D042763:Acidithiobacillus / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D000217:Acyltransferases / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006032:Glycine-tRNA Ligase / Q000235:genetics; D007501:Iron / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D009876:Operon; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics; D012329:RNA, Bacterial / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017421:Sequence Analysis; D016962:Sinorhizobium meliloti / Q000187:drug effects / Q000502:physiology; D013455:Sulfur / Q000378:metabolism
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012333:RNA, Messenger; C088386:homoserine lactone; D013455:Sulfur; D007501:Iron; D000217:Acyltransferases; D010744:Phosphoric Monoester Hydrolases; D006032:Glycine-tRNA Ligase; D015107:4-Butyrolactone