Expression of immune-related genes in the oyster Crassostrea gigas during ontogenesis.
The work presented here reports the expression of immune-related genes during ontogenesis in the oyster Crassostrea gigas. Expression patterns of 18 selected genes showed that RNAs detected in oocytes and 2-4 cell embryos are of maternal origin and that gene transcription starts early after fertilization. The expression patterns of 4 genes (Cg-timp, Cg-tal, Cg-EcSOD and Drac3) suggested that hemocytes appear in the gastrula-trochophore stages. The localization of Cg-tal expression suggested that hematopoietic cells were derived from vessels and/or artery endothelia cells. Moreover, a bacterial challenge affected the level of expression of genes. Indeed, a change in expression levels was observed for Cg-LBP/BPI, Cg-timp, Drac3 and Cg-MyD88 genes in larval stages upon exposure to non-pathogenic bacteria. In early juveniles, a modulation was also observed for Cg-LBP/BPI, Cg-timp, Cg-MyD88 and for Cg-tal, according to the concentration of bacteria. Altogether, the results showed that studying the appearance of immunocompetent cells through their ability to express immune-related genes is a tool to gain insight the ontogenesis of the oyster immune system.
Tirapé
Ana
A
UMR5171, IFREMER, CNRS, UMII, Génome Populations Interactions Adaptation (GPIA), Université de Montpellier II, 2 Place Eugène Bataillon, CC80, 34095 Montpellier Cedex 5, France.
Bacque
Coralie
C
Brizard
Raphaël
R
Vandenbulcke
Franck
F
Boulo
Viviane
V
D000820:Animal Diseases / Q000235:genetics / Q000382:microbiology / Q000473:pathology; D000818:Animals; D001424:Bacterial Infections / Q000235:genetics / Q000382:microbiology / Q000473:pathology; D049896:Crassostrea / Q000196:embryology / Q000235:genetics* / Q000254:growth & development / Q000276:immunology*; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D019714:Tissue Inhibitor of Metalloproteinases / Q000235:genetics / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism; D020744:rac GTP-Binding Proteins / Q000235:genetics
D012333:RNA, Messenger; D019714:Tissue Inhibitor of Metalloproteinases; D013482:Superoxide Dismutase; D044767:Ubiquitin-Protein Ligases; D020744:rac GTP-Binding Proteins