High-level expression and purification of an analgesic peptide from Buthus martensii Karch.
BmK AngM1, a scorpion peptide isolated from Buthus martensii Karch was reported to exhibit potential analgesic effect. But the relative low content of this toxin in crude venom limits its further characterization. In this study, we constructed an expression vector and transformed into E.coli. The BmK AngM1 was expressed as a fusion protein in the soluble fraction and was purified by Nickel affinity chromatography. Subsequently, the purified fusion protein was cleaved by enterokinase and was further purified by reverse-phase HPLC. We purified 25 mg recombinant BmK AngM1 (rBmK AngM1) from 1 L bacterial culture. The molecular weight of rBmK AngM1 determined by ESI-MS was 7240.4 Da which was the expected size for correctly processed. Analgesic bioassay studies of rBmK AngM1 exhibited its potential analgesic effect comparable to that of the natural BmK AngM1 peptide.
Cao
Z
Z
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, The People's Republic of China. zycao1999@hotmail.com
Wang
W
W
Xiao
X
X
Chen
K
K
Liang
X
X
Yu
D
D
D000595:Amino Acid Sequence; D000700:Analgesics / Q000009:adverse effects / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D010455:Peptides / Q000009:adverse effects / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000009:adverse effects / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012605:Scorpions / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D000700:Analgesics; D010455:Peptides; D011993:Recombinant Fusion Proteins