In vitro evaluation of magnetic resonance imaging at 3.0 tesla on clonogenic ability, proliferation, and cell cycle in human embryonic lung fibroblasts.
OBJECTIVE
We investigated the influence of magnetic resonance (MR) at 3.0 T on clonogenic ability, proliferation, and cell cycle in an embryonic human cell line.


METHODS
Cells (human lung fibroblasts Hel 299) were exposed to the static magnetic field (3.0 T) of a magnetic resonance imager (MRI) and to a turbo spin echo sequence at 3.0 T within clinical limitations (specific absorption rate 0.92 W/kg). A special MR-compatible incubation system was used. A control group (sham-exposed) and a MRI group (exposed) were set up. We investigated 3 biologic endpoints: colony forming, cell cycle, and proliferation ability. The exposure time was 2 hours in each experiment.


RESULTS
In the statistical analysis, none of these tests showed relevant differences between the exposed and sham-exposed group.


CONCLUSIONS
No influences of the static field alone as well as a turbo spin echo sequence at 3.0 T on clonogenic ability, proliferation, or cell cycle in eugenic human lung fibroblasts were found.
Schwenzer
Nina F
NF
Department of Diagnostic Radiology, Eberhard-Karls University, Tübingen, Germany. nina.schwenzer@med.uni-tuebingen.de
Bantleon
Rüdiger
R
Maurer
Brigitte
B
Kehlbach
Rainer
R
Herberts
Tina
T
Claussen
Claus D
CD
Rodegerdts
Enno
E
D002453:Cell Cycle / Q000502:physiology*; D049109:Cell Proliferation; D004574:Electromagnetic Fields / Q000009:adverse effects*; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D008168:Lung / Q000502:physiology*; D008279:Magnetic Resonance Imaging / Q000009:adverse effects / Q000295:instrumentation*; D013234:Stem Cells