Modulation of doxorubicin-induced genotoxicity by Aegle marmelos in mouse bone marrow: a micronucleus study.
The effect of various concentrations of Aegle marmelos (AME) on the doxorubicin (DOX)-induced genotoxic effects in mice bone marrow was studied. Treatment of mice with different concentrations of DOX resulted in a dose-dependent elevation in the frequency of micronucleated polychromatic (MPCE) as well as normochromatic (MNCE) erythrocytes in mouse bone marrow. The frequencies of MPCE and MNCE increased with scoring time, and the greatest elevation for MPCE was observed at 48 hours post-DOX treatment, whereas a maximum increase in MNCE was observed at 72 hours post-DOX treatment. This increase in MPCE and MNCE was accompanied by a decline in the polychromatic erythrocytes-normochromatic erythrocytes (PCE/NCE) ratio, which showed a DOX-dose-dependent decline. Treatment of mice with 200, 250, 300, 350, and 400 mg/kg body weight of AME, orally once daily for 5 consecutive days before DOX treatment, significantly reduced the frequency of DOX-induced micronuclei accompanied by a significant elevation in the PCE/NCE ratio at all scoring times. The greatest protection against DOX-induced genotoxicity was observed at 350 mg/kg AME. The protection against DOX-induced genotoxicity by AME may be due to inhibition of free radicals and increased antioxidant status.
Venkatesh
Ponemone
P
Department of Radiobiology, Kasturba Medical College, Manipal, India; Department of Human Nutrition, College of Applied Health Sciences, 1919 West Taylor Street, MC 517, University of Illinois at Chicago, Chicago, IL 60612. USA. ponemone@uic.edu
Shantala
Bellary
B
Jagetia
Ganesh Chandra
GC
Rao
K Koteshwer
KK
Baliga
Manjeshwar Shrinath
MS
D045471:Aegle; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity*; D001853:Bone Marrow / Q000187:drug effects*; D004249:DNA Damage / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000633:toxicity*; D004912:Erythrocytes / Q000187:drug effects; D005609:Free Radicals; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology
D000903:Antibiotics, Antineoplastic; D005609:Free Radicals; D010936:Plant Extracts; D004317:Doxorubicin