[Sequence polymorphism of the promoter region of gene STK11 in patients with Peutz-Jeghers syndrome].
OBJECTIVE
To explore the relationship between the sequence variation of the promoter region (-1543 approximately -1160) of STK11 gene and the risk of developing Peutz-Jeghers syndrome (PJS).


METHODS
The sequences of the promoter region of 14 PJS patients (7 patients are inherited and the other 7 patients are sporadic) and 42 normal individuals were PCR amplified and then sequenced.


RESULTS
A new single nucleotide polymorphism (SNP) G/T (-1275) in STK11 promoter region was identified. The frequency of genotype GG, GT, and TT was 53.3%, 26.7%, and 20%, respectively among PJS patients and 33.3%, 64.3%, and 2.4%, respectively among the normal individuals. The frequency of genotype GG and TT among patients was significantly higher than that among the normal individuals, and the frequency of genotype GT among patients was significantly lower than that among the normal individuals (chi(2)=8.521, P<0.05).


CONCLUSIONS
G/T(-1275) in STK11 promoter region is a new SNP. The genotype of this new SNP may relate to the risk of developing Peutz-Jeghers syndrome (PJS) deserve further research.
Yi
Xin
X
Department of Gastroenterology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Yao
Mao-Jin
MJ
Wang
Yong-Jun
YJ
Tang
Jian-Guang
JG
Ning
Wen-Feng
WF
Wang
Xiang-Ping
XP
Zhou
Shi-Quan
SQ
Li
Chen-Jie
CJ
Wang
Fei
F
Xia
Kun
K
Shi
Xiao-Liu
XL
D000091162:AMP-Activated Protein Kinase Kinases; D001483:Base Sequence; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D010580:Peutz-Jeghers Syndrome / Q000235:genetics*; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*
D017346:Protein Serine-Threonine Kinases; C109790:STK11 protein, human; D000091162:AMP-Activated Protein Kinase Kinases