title: Treatment with beta2-adrenoceptor agonist in vivo induces human clock gene, Per1, mRNA expression in peripheral blood.
abstract: This study examined whether in vivo exposure to a beta2-adrenoceptor agonist, tulobuterol, induces human Period1 (hPer1) mRNA expression in cells from peripheral whole blood. In one experiment, oral tulobuterol was administered to five healthy volunteers at 22:00 h, while in another, a transdermally tulobuterol patch was applied to the same five subjects at 20:00 h. In each experiment, serum tulobuterol concentrations were measured at four time points, and total RNA was isolated from peripheral blood cells for determinations of hPer1 mRNA expression by real-time polymerase chain reaction. Both the tulobuterol tablet and the transdermal patch increased hPer1 mRNA expression, suggesting that analyses of human peripheral blood cells could reliably represent peripheral clock gene mRNA expression in vivo.
authors.affiliation: Division of Medical Oncology and Molecular Respirology, Tottori University, Nishimachi, Yonago, Japan. burioka@grape.med.tottori-u.ac.jp
authors.forename: Naoto
authors.initials: N
authors.lastname: Burioka
authors.forename: Miyako
authors.initials: M
authors.lastname: Takata
authors.forename: Masahiro
authors.initials: M
authors.lastname: Endo
authors.forename: Masanori
authors.initials: M
authors.lastname: Miyata
authors.forename: Kenichi
authors.initials: K
authors.lastname: Takeda
authors.forename: Hiroki
authors.initials: H
authors.lastname: Chikumi
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Tomita
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Fukuoka
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Nakazaki
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sano
authors.forename: Eiji
authors.initials: E
authors.lastname: Shimizu
mesh_terms: D000318:Adrenergic beta-Agonists / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D000328:Adult; D018797:Cell Cycle Proteins / Q000097:blood* / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D008297:Male; D056950:Period Circadian Proteins; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics; D013726:Terbutaline / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood / Q000494:pharmacology
chemical_list: D000318:Adrenergic beta-Agonists; D018797:Cell Cycle Proteins; C539022:PER1 protein, human; D056950:Period Circadian Proteins; D012333:RNA, Messenger; C010809:tulobuterol; D013726:Terbutaline