Suppressive effects of Chelidonium majus methanol extract in knee joint, regional lymph nodes, and spleen on collagen-induced arthritis in mice.
Chelidonium majus L. has multiple applications in Korean traditional medicine because of its anti-tumoral, cytotoxic, anti-inflammatory and anti-microbial activities and has long been known to have anti-inflammatory effects. However, no study on the anti-arthritic activity of Chelidonium majus has been reported in vivo. Rheumatoid arthritis (RA) is a systemic autoimmune disease with chronic inflammation characterized by hyperplasia of synovial cells in affected joints, which ultimately leads to the destruction of cartilage and bone. Cytokine production and gene expression were assessed during CIA (collagen-induced arthritis) model mice in knee joint, lymph node (LN), and spleen, using ELISA and competitive RT-PCR. DBA/1J mice were immunized with bovine type II collagen. After a second collagen immunization, mice were treated with CME orally at 400, 40mg/kg once a day for 4 weeks. The severity of arthritis within the knee joints was evaluated by histological assessment of cartilage destruction and pannus formation. Administration of CME significantly suppressed the progression of CIA and inhibited the production of TNF-alpha and IL-6 in spleen and lymph node. The erosion of cartilage was dramatically reduced in mouse knees after treatment with CME. In conclusion, our results demonstrates that CME significantly suppressed the progression of CIA and that this action was characterized by the decreased production of TNF-alpha, IL-6, IFN-gamma, B cells, gammadelta T cells (in spleen) and increased proportion of CD4+CD25+ regulatory T cells in vivo. In the serum of CME-treated mice, the levels of IgG and IgM RA factor were decreased.
Lee
Young-Cheol
YC
Department of Herbology, College of Oriental Medicine, Sangji University, Wonju 220-702, Republic of Korea. lyc072@sangji.ac.kr
Kim
Seung-Hyung
SH
Roh
Seong-Soo
SS
Choi
Ho-Young
HY
Seo
Young-Bae
YB
D000818:Animals; D001169:Arthritis, Experimental / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D028281:Chelidonium; D016207:Cytokines / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression / Q000187:drug effects; D006614:Hindlimb; D007155:Immunologic Factors / Q000494:pharmacology*; D007596:Joints / Q000187:drug effects* / Q000473:pathology; D007723:Korea; D008198:Lymph Nodes / Q000187:drug effects* / Q000473:pathology; D008297:Male; D008518:Medicine, East Asian Traditional; D000432:Methanol; D051379:Mice; D008811:Mice, Inbred DBA; D010936:Plant Extracts / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012997:Solvents; D013154:Spleen / Q000187:drug effects* / Q000473:pathology; D013601:T-Lymphocytes / Q000378:metabolism
D016207:Cytokines; D007155:Immunologic Factors; D010936:Plant Extracts; D012997:Solvents; D000432:Methanol