Study on the compatibility of cefotaxime with tinidazole in glucose injection.
An efficient HPLC method for the compatibility study of cefotaxime with tinidazole in glucose injection is described, which has been developed for the simultaneous determination of cefotaxime and tinidazole in glucose injection. The appearance and pH value of the mixed solution were investigated and the concentrations of cefotaxime and tinidazole were determined by RP-HPLC with an Agilent ZORBAX Eclipse XDB-C8 column, gradient elution and dual wavelength detection on diode-array-detector (DAD) at room temperature (20 degrees C) within 24 h. It was found that the resulting appearance and pH value of the mixed solution showed slight changes, on the other hand, the quantity of cefotaxime decreased significantly. The results show that the mixed solution of cefotaxime with tinidazole in glucose injection must be used within 8 h in clinical due to the possible degradation of cefotaxime in tinidazole glucose injection. This study provides a convenient method for rational use of compatible drugs in clinical practice.
Guo
Peng
P
State Key Laboratory of Virology, Wuhan 430072, PR China. guopeng@whu.edu.cn
Li
Xiaohua
X
Wang
Jian
J
You
An
A
D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000032:analysis / Q000737:chemistry; D000890:Anti-Infective Agents / Q000737:chemistry; D000981:Antiprotozoal Agents / Q000737:chemistry; D000994:Antitrichomonal Agents / Q000008:administration & dosage* / Q000032:analysis / Q000737:chemistry; D002439:Cefotaxime / Q000008:administration & dosage* / Q000032:analysis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D004344:Drug Incompatibility; D004355:Drug Stability; D005947:Glucose / Q000008:administration & dosage*; D006863:Hydrogen-Ion Concentration; D007267:Injections; D008795:Metronidazole / Q000737:chemistry; D015394:Molecular Structure; D012015:Reference Standards; D012680:Sensitivity and Specificity; D012995:Solubility; D012996:Solutions / Q000737:chemistry; D013696:Temperature; D013997:Time Factors; D014011:Tinidazole / Q000008:administration & dosage* / Q000032:analysis / Q000737:chemistry; D014867:Water / Q000737:chemistry
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D000981:Antiprotozoal Agents; D000994:Antitrichomonal Agents; D012996:Solutions; D014011:Tinidazole; D014867:Water; D008795:Metronidazole; D005947:Glucose; D002439:Cefotaxime