Dynamics of the readily releasable pool during post-tetanic potentiation in the rat calyx of Held synapse.
The size of the readily releasable pool (RRP) of vesicles was measured in control conditions and during post-tetanic potentiation (PTP) in a large glutamatergic terminal called the calyx of Held. We measured excitatory postsynaptic currents evoked by a high frequency train of action potentials in slices of 4-11-day-old rats. After a tetanus the cumulative release during such a train was enlarged by approximately 50%, indicating that the size of the RRP was increased. The amount of enhancement depended on the duration and frequency of the tetanus and on the age of the rat. After the tetanus, the size of the RRP decayed more slowly (t(1/2)=10 versus 3 min) back to control values than the release probability. This difference was mainly due to a very fast initial decay of the release probability, which had a time constant compatible with an augmentation phase (tau approximately 30 s). The overall decay of PTP at physiological temperature was not different from room temperature, but the increase in release probability (P(r)) was restricted to the first minute after the tetanus. Thereafter PTP was dominated by an increase in the size of the RRP. We conclude that due to the short lifetime of the increase in release probability, the contribution of the increase in RRP size during post-tetanic potentiation is more significant at physiological temperature.
Habets
Ron L P
RL
Department of Neuroscience, Erasmus MC, University Medical Center Rotterdam, Dr Molewaterplein 50, 3015 GE Rotterdam, the Netherlands.
Borst
J Gerard G
JG
D000200:Action Potentials; D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D017626:Cochlear Nucleus / Q000166:cytology / Q000378:metabolism*; D004558:Electric Stimulation / Q000379:methods; D019706:Excitatory Postsynaptic Potentials; D005973:Glutamine / Q000378:metabolism*; D006207:Half-Life; D066298:In Vitro Techniques; D007700:Kinetics; D009473:Neuronal Plasticity; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar; D009435:Synaptic Transmission / Q000502:physiology*; D013572:Synaptic Vesicles / Q000378:metabolism*; D013696:Temperature
D005973:Glutamine