Adherence and invasion of mouse-adapted H pylori in different epithelial cell lines.
OBJECTIVE
To assess the adhesion and invasion abilities of different mouse adapted H pylori strains in different cell lines in vitro and investigate their effects on the virulence factors cagA and vacA.


METHODS
The adherence and invasion abilities of different H pylori strains in different epithelial cell lines were examined by the gentamycin protection assay. The null mutants of cagA and vacA were processed by direct PCR mutation method. The morphologic changes of different cell lines after H pylori attachment were examined by microscopy.


RESULTS
The densities of adherence to and invasion into cells in vitro were different from those in the mouse infection experiments. 88-3887 strain could invade and adhere to cells stronger than SS1 and X47. All tested strains had better adhering and invasive abilities in SCG-7901 cell. CagA and vacA minus mutants had the same invasion and adherent abilities as their wild types. In all strains and cell lines tested, only AGS cell had the significant hummingbird phenotype after inoculation with the 88-3887 wild-type.


CONCLUSIONS
Both the host cells and the bacteria play important parts in the invasion and adhesion abilities of H pylori. CagA and VacA are not related to the ability of invasion and adhesion of H pylori in different cell lines in vitro.
Zhang
Mao-Jun
MJ
Department of Diagnostics, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, PO Box 5, Changping, Beijing 102206, China.
Meng
Fan-Liang
FL
Ji
Xiao-Yun
XY
He
Li-Hua
LH
Zhang
Jian-Zhong
JZ
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000942:Antigens, Bacterial / Q000235:genetics / Q000502:physiology; D001422:Bacterial Adhesion / Q000187:drug effects / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D002460:Cell Line; D045744:Cell Line, Tumor; D004285:Dogs; D004847:Epithelial Cells / Q000166:cytology / Q000382:microbiology*; D005839:Gentamicins / Q000494:pharmacology; D016481:Helicobacter Infections / Q000503:physiopathology; D016480:Helicobacter pylori / Q000472:pathogenicity* / Q000502:physiology*; D006801:Humans; D051379:Mice; D009154:Mutation / Q000235:genetics
D000900:Anti-Bacterial Agents; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D005839:Gentamicins; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori