title: Back-stepping, hidden substeps, and conditional dwell times in molecular motors.
abstract: Processive molecular motors take more-or-less uniformly sized steps, along spatially periodic tracks, mostly forwards but increasingly backwards under loads. Experimentally, the major steps can be resolved clearly within the noise but one knows biochemically that one or more mechanochemical substeps remain hidden in each enzymatic cycle. In order to properly interpret experimental data for back-to-forward step ratios, mean conditional step-to-step dwell times, etc., a first-passage analysis has been developed that takes account of hidden substeps in N -state sequential models. The explicit, general results differ significantly from previous treatments that identify the observed steps with complete mechanochemical cycles; e.g., the mean dwell times tau(+) and tau(-) prior to forward and back steps, respectively, are normally unequal although the dwell times tau(++) and tau(--) between successive forward and back steps are equal. Illustrative (N=2) -state examples display a wide range of behavior. The formulation extends to the case of two or more detectable transitions in a multistate cycle with hidden substeps.
authors.affiliation: Institute for Physical Science and Technology, University of Maryland, College Park, Maryland 20742, USA. dtsygank@umd.edu
authors.forename: Denis
authors.initials: D
authors.lastname: Tsygankov
authors.forename: Martin
authors.initials: M
authors.lastname: Lindén
authors.forename: Michael E
authors.initials: ME
authors.lastname: Fisher
mesh_terms: D001665:Binding Sites; D003198:Computer Simulation; D008954:Models, Biological; D008956:Models, Chemical; D008958:Models, Molecular; D020409:Molecular Motor Proteins / Q000737:chemistry* / Q000502:physiology*; D009038:Motion; D009068:Movement / Q000502:physiology*; D011485:Protein Binding
chemical_list: D020409:Molecular Motor Proteins