title: [The methodological aspects of off-line sampling of exhaled air for nitric oxide measurement in Japanese].
abstract: The measurement of exhaled nitric oxide (eNO) is a non-invasive biomarker of bronchial inflammation. Despite the usefulness of eNO measurement, NO analyzers are too expensive for widespread use by general practitioners. In comparison, the off-line (bag collection) method of eNO measurement may be more useful. In Japan, however, there have been few studies about eNO in asthmatics using the off-line method. This study shows methodological aspects of the off-line method. Briefly, with a SIEVERS bag collection kit, we recommend that the flow rate and pressure level of exhaled air should be 70 ml/sec and 10 cm H2O, respectively and that the sampled air should be measured within 12 hours.
authors.affiliation: Clinical Research Center for Allergy and Rheumatology, National Hospital Organization, Sagamihara National Hospital.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Tsuburai
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Suzuki
authors.forename: Naomi
authors.initials: N
authors.lastname: Tsurikisawa
authors.forename: Noritaka
authors.initials: N
authors.lastname: Higashi
authors.forename: Ai
authors.initials: A
authors.lastname: Higashi
authors.forename: Chiyako
authors.initials: C
authors.lastname: Oshikata
authors.forename: Emiko
authors.initials: E
authors.lastname: Ono
authors.forename: Sonoko
authors.initials: S
authors.lastname: Morita
authors.forename: Mamoru
authors.initials: M
authors.lastname: Ohtomo
authors.forename: Yuji
authors.initials: Y
authors.lastname: Maeda
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Ikehara
authors.forename: Masami
authors.initials: M
authors.lastname: Taniguchi
authors.forename: Kazuo
authors.initials: K
authors.lastname: Akiyama
mesh_terms: D000328:Adult; D001944:Breath Tests / Q000295:instrumentation; D001991:Bronchitis / Q000175:diagnosis* / Q000473:pathology; D045853:Exhalation / Q000502:physiology*; D005260:Female; D006801:Humans; D007249:Inflammation / Q000175:diagnosis; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000032:analysis*; D012494:Sampling Studies
chemical_list: D009569:Nitric Oxide