Heterochromatin protein 1 interacts with 5'UTR of transposable element ZAM in a sequence-specific fashion.
The realization of cross talks between transposable elements of class I and their host genome involves non-histonic chromatin proteins. These interactions have been widely analyzed through the characterization of the gypsy retrotransposon leader region, which holds a particularly strong insulator element, and the proteins required for its function, Su(Hw), Mod(mdg4), and Cp190. Here we provide evidence that a similar interaction should occur between ZAM, a gypsy-like element, and HP1, one of the most extensively studied chromatin proteins. We first assayed the existence of this binding using the yeast cells one-hybrid system and then we verified it in vivo by ChIP assay. In order to characterize the interaction between HP1 and the ZAM 5' untranslated region we performed a series of gel shift analyses. Our observations confirm an HP1 co-operative DNA-binding and display for the first time the HP1 DNA target motif that, we hypothesize, should be one of its nucleation sites.
Minervini
Crescenzio Francesco
CF
Dipartimento di Genetica e Microbiologia, Universita' di Bari, 70126 Bari, Italy.
Marsano
Rene' Massimiliano
RM
Casieri
Paola
P
Fanti
Laura
L
Caizzi
Ruggiero
R
Pimpinelli
Sergio
S
Rocchi
Mariano
M
Viggiano
Luigi
L
D020121:5' Untranslated Regions / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D000090266:Chromobox Protein Homolog 5; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D004251:DNA Transposable Elements / Q000235:genetics*; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D011485:Protein Binding; D018626:Retroelements / Q000235:genetics; D020080:Tandem Repeat Sequences / Q000235:genetics
D020121:5' Untranslated Regions; D002868:Chromosomal Proteins, Non-Histone; D004251:DNA Transposable Elements; D029721:Drosophila Proteins; D018626:Retroelements; D000090266:Chromobox Protein Homolog 5