title: Agarose-gelatin conjugate for adherent cell-enclosing capsules.
abstract: Spherical capsules were prepared by extruding aqueous agarose-gelation conjugate solution into co-flowing liquid paraffin at 38 degrees C and cooling the resultant emulsion. Capsule diameter was controlled between 40 and 250 mum by changing the velocity of the liquid paraffin. Adherent Crandall-Reese feline kidney cells enclosed in conjugate capsules of 141 +/- 23 mum diam. had a higher degree of proliferation than those in unmodified agarose capsules. Mitochondrial activity, detected for cell-enclosing conjugate capsules normalized against unit volume of gel, was about double that of unmodified agarose capsules over 28 days. These results demonstrated the feasibility of agarose-gelatin conjugate as a material of cell-enclosing capsules.
authors.affiliation: Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan. sakai@chem-eng.kyushu-u.ac.jp
authors.forename: Shinji
authors.initials: S
authors.lastname: Sakai
authors.forename: Ichiro
authors.initials: I
authors.lastname: Hashimoto
authors.forename: Koei
authors.initials: K
authors.lastname: Kawakami
mesh_terms: D000818:Animals; D002214:Capsules / Q000378:metabolism*; D002415:Cats; D002448:Cell Adhesion; D002460:Cell Line; D005780:Gelatin / Q000378:metabolism*; D008899:Mineral Oil; D008928:Mitochondria / Q000378:metabolism; D010316:Particle Size; D012685:Sepharose / Q000378:metabolism*
chemical_list: D002214:Capsules; D008899:Mineral Oil; D005780:Gelatin; D012685:Sepharose