Neoglycopeptide dendrimer libraries as a source of lectin binding ligands.
[structure: see text] A 15 625-membered peptide dendrimer combinatorial library was acylated with an alpha-C-fucosyl residue at its four N-termini and screened for binding to fucose-specific lectins. Dendrimer FD2 (Fuc-alpha-CH2CO-Lys-Pro-Leu)4(Lys-Phe-Lys-Ile)2Lys-His-Ile-NH2 was identified as a potent ligand against Ulex europaeus lectin UEA-I (IC50 = 11 microM) and Pseudomonas aeruginosa lectin PA-IIL (IC50 = 0.14 microM).
Kolomiets
Elena
E
Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, CH-3012, Berne, Switzerland.
Johansson
Emma M V
EM
Renaudet
Olivier
O
Darbre
Tamis
T
Reymond
Jean-Louis
JL
D030562:Databases, Protein; D050091:Dendrimers / Q000737:chemistry* / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005643:Fucose / Q000737:chemistry; D006020:Glycopeptides / Q000737:chemistry* / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism*; D008024:Ligands; D015394:Molecular Structure; D011485:Protein Binding
D050091:Dendrimers; D006020:Glycopeptides; D037102:Lectins; D008024:Ligands; D005643:Fucose