title: [A new molecular method to authenticate radix trichosanthis as well as its adulterants and substitutes].
abstract: OBJECTIVE
  To explore a new molecular method to authenticate Radix Trichosanthis.
  
  
  METHODS
  Three 20 mer primers based on the ITS sequence was designed. The PCR reaction system was optimized and applied to nineteen different sources of Radix Trichosanthis and nine adulterants and substitutes.
  
  
  RESULTS
  Polymorphic map of Radix Trichosanthis and its adulterants was obtained from primer TKS1-64. 560 bp and 960 bp bands were authentic markers for Radix Trichosanthis.
  
  
  CONCLUSIONS
  Primer TKS1-64F possesses the advantages of good stability and reproducibility. This new method is named as anchored primer amplification polymorphism DNA(APAPD). It was a potential method to used in molecular identification of other meteria medica.
authors.affiliation: Institute of Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Guang-Hong
authors.initials: GH
authors.lastname: Cui
authors.forename: Lu-Qi
authors.initials: LQ
authors.lastname: Huang
authors.forename: Min
authors.initials: M
authors.lastname: Wang
mesh_terms: D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics; D021903:DNA, Ribosomal Spacer; D004340:Drug Contamination / Q000517:prevention & control; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods; D015203:Reproducibility of Results; D029778:Trichosanthes / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D015978:Trichosanthin / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer; D015978:Trichosanthin