Dynamics of replication-independent histone turnover in budding yeast.
Chromatin plays roles in processes governed by different time scales. To assay the dynamic behavior of chromatin in living cells, we used genomic tiling arrays to measure histone H3 turnover in G1-arrested Saccharomyces cerevisiae at single-nucleosome resolution over 4% of the genome, and at lower (approximately 265 base pair) resolution over the entire genome. We find that nucleosomes at promoters are replaced more rapidly than at coding regions and that replacement rates over coding regions correlate with polymerase density. In addition, rapid histone turnover is found at known chromatin boundary elements. These results suggest that rapid histone turnover serves to functionally separate chromatin domains and prevent spread of histone states.
Dion
Michael F
MF
Faculty of Arts and Sciences, Center for Systems Biology, Harvard University, Cambridge, MA 02138, USA.
Kaplan
Tommy
T
Kim
Minkyu
M
Buratowski
Stephen
S
Friedman
Nir
N
Rando
Oliver J
OJ
D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D004261:DNA Replication; D016193:G1 Phase; D005800:Genes, Fungal; D016681:Genome, Fungal; D006657:Histones / Q000378:metabolism*; D007700:Kinetics; D009707:Nucleosomes / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D024363:Transcription Initiation Site
D002843:Chromatin; D006657:Histones; D009707:Nucleosomes; D029701:Saccharomyces cerevisiae Proteins; D012319:RNA Polymerase II