Distribution and genetic variability among Campylobacter spp. isolates from different animal species and humans in Switzerland.
In Switzerland, a national database with 1028 Campylobacter isolates from poultry, pigs, cats, dogs, cattle, humans, zoo animals and water has been created. The database contains the genetic fingerprint and background information of each Campylobacter isolate. Dominant species could be identified in the different sources with a majority of Campylobacter jejuni in poultry (73%), humans (79%), cattle (95%), zoo animals (40%) and water (100%), of Campylobacter coli in pigs (72%), and of Campylobacter upsaliensis/helveticus in cats and dogs (55%). The comparison of three genotyping methods, amplified fragment length polymorphism (AFLP), pulsed field gel electrophoresis and restriction fragment length polymorphism, revealed that AFLP allows discrimination between the different Campylobacter species and is the most appropriate method to distinguish specific strains within the same species. Genotyping analysis demonstrated that the Campylobacter population is heterogeneous among the different sources and that no dominant clone is spread in the country. Genotyping and the resulting database are useful tools to trace back future Campylobacter infections.
Keller
J
J
Institute of Veterinary Bacteriology, University of Bern, Länggassstrasse 122, CH-3001 Bern, Switzerland.
Wieland
B
B
Wittwer
M
M
Stephan
R
R
Perreten
V
V
D000818:Animals; D002167:Campylobacter / Q000145:classification* / Q000235:genetics*; D016172:DNA Fingerprinting / Q000379:methods / Q000662:veterinary; D030541:Databases, Genetic; D016521:Electrophoresis, Gel, Pulsed-Field; D005784:Gene Amplification; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity; D013557:Switzerland