Effects of differential extraction of Verbena officinalis on rat models of inflammation, cicatrization and gastric damage.
Verbena officinalis L. is used in folk medicine for the treatment of inflammatory disorders, skin burns, abrasions, and gastric diseases. Extracts obtained with different solvents (methanol, VoME; enriched flavonoids, VoEF; supercritical CO2, VoCO2) were evaluated for anti-inflammatory, gastroprotective and cicatrizing activities. Additionally, the antioxidant capacity was determined in vitro. In order to confirm the activities investigated, histological observations were performed. All extracts induce a remarkable anti-inflammatory activity. The gastric damage is significantly reduced by all extracts administered, whereby the most pronounced protection is observed for the VoCO2 and VoEF extracts. Finally, a wound healing effect is obtained particularly by the CO2 extract, suggesting the presence of some lipophilic active principles. Histological evidence confirms the results evaluated with the animal procedures. The results obtained after oral administration of V. officinalis extracts are also in agreement with the antioxidant capacity evaluated in vitro, confirming the relationship between pharmacological activities and antiradical efficacy.
Speroni
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E
Dipartimento di Farmacologia, Università di Bologna, Bologna, Italy. ester.speroni@unibo.it
Cervellati
R
R
Costa
S
S
Guerra
M C
MC
Utan
A
A
Govoni
P
P
Berger
A
A
Müller
A
A
Stuppner
H
H
D000818:Animals; D000897:Anti-Ulcer Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D002351:Carrageenan; D002921:Cicatrix / Q000517:prevention & control; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control; D008297:Male; D016595:Misoprostol; D008517:Phytotherapy; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D013276:Stomach Ulcer / Q000139:chemically induced / Q000517:prevention & control*; D032412:Verbena; D014945:Wound Healing / Q000187:drug effects
D000897:Anti-Ulcer Agents; D000975:Antioxidants; D010936:Plant Extracts; D016595:Misoprostol; D002351:Carrageenan