title: Differences in phenotypic and genotypic traits against antimicrobial agents between Acinetobacter baumannii and Acinetobacter genomic species 13TU.
abstract: OBJECTIVE
  To investigate the differences in antimicrobial susceptibility and resistance mechanisms against imipenem between Acinetobacter baumannii and Acinetobacter genomic species 13TU.
  
  
  METHODS
  A total of 232 non-duplicate Acinetobacter species were consecutively collected from two Korean hospitals in Daegu, Republic of Korea, between November 2004 and November 2005. Antimicrobial susceptibility was determined by agar dilution methods. Resistance to imipenem was characterized by a carbapenemase activity test and PCR amplification. PFGE was performed to determine the clonal relatedness of imipenem-resistant Acinetobacter species.
  
  
  RESULTS
  A. baumannii was the most prevalent species (61.2%), followed by Acinetobacter genomic species 13TU (25.9%). The resistance rates of A. baumannii to most antimicrobial agents were higher than those of other Acinetobacter species, while the resistance rate to imipenem was the highest in Acinetobacter genomic species 13TU. Imipenem-resistant Acinetobacter genomic species 13TU isolates produced VIM-2 metallo-beta-lactamase, while imipenem-resistant A. baumannii isolates produced OXA-23 and/or OXA-51 beta-lactamase. Imipenem-resistant Acinetobacter strains originated from different clones in each hospital.
  
  
  CONCLUSIONS
  Two prevalent Acinetobacter species, A. baumannii and Acinetobacter genomic species 13TU, possess distinct phenotypic and genotypic traits against antimicrobials.
authors.affiliation: Department of Microbiology, Kyungpook National University School of Medicine, Daegu 700-422, Republic of Korea.
authors.forename: Jung Hoon
authors.initials: JH
authors.lastname: Lee
authors.forename: Chul Hee
authors.initials: CH
authors.lastname: Choi
authors.forename: Hee Young
authors.initials: HY
authors.lastname: Kang
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Jungmin
authors.initials: J
authors.lastname: Kim
authors.forename: Yoo Chul
authors.initials: YC
authors.lastname: Lee
authors.forename: Sung Yong
authors.initials: SY
authors.lastname: Seol
authors.forename: Dong Taek
authors.initials: DT
authors.lastname: Cho
authors.forename: Kun Woo
authors.initials: KW
authors.lastname: Kim
authors.forename: Do Young
authors.initials: DY
authors.lastname: Song
authors.forename: Je Chul
authors.initials: JC
authors.lastname: Lee
mesh_terms: D000150:Acinetobacter / Q000187:drug effects* / Q000235:genetics*; D040981:Acinetobacter baumannii / Q000187:drug effects* / Q000235:genetics*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000378:metabolism; D015780:Carbapenems / Q000378:metabolism; D024881:Drug Resistance, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D016680:Genome, Bacterial / Q000235:genetics; D005838:Genotype; D006801:Humans; D015378:Imipenem / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001618:beta-Lactamases / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D015378:Imipenem; D001618:beta-Lactamases; C063912:carbapenemase