New triphosphate conjugates bearing reporter groups: labeling of DNA fragments for microarray analysis.
A simple and convenient method for incorporation of fluorescent or ligand groups into 3'-termini of DNA fragments is proposed. A set of triphosphoric acid monoesters bearing fluorescent groups or biotin attached to the triphosphate fragment through linkers of different lengths and structures was synthesized. All the compounds were substrates for calf thymus terminal deoxynucleotidyltransferase and were used for incorporation of marker groups into 3'-termini of DNA fragments. The compounds were successfully applied for DNA labeling during post-PCR target preparation for microarray analysis.
Alexandrova
Ludmila A
LA
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Street, Moscow 119991, Russian Federation.
Jasko
Maxim V
MV
Belobritskaya
Elena E
EE
Chudinov
Alexander V
AV
Mityaeva
Olga N
ON
Nasedkina
Tatyana V
TV
Zasedatelev
Alexander S
AS
Kukhanova
Marina K
MK
D000818:Animals; D001710:Biotin / Q000737:chemistry; D002417:Cattle; D004253:DNA Nucleotidylexotransferase / Q000737:chemistry; D015342:DNA Probes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D011122:Polyphosphates / Q000737:chemistry*
D015342:DNA Probes; D005456:Fluorescent Dyes; D011122:Polyphosphates; D001710:Biotin; D004253:DNA Nucleotidylexotransferase; C005692:triphosphoric acid