Analyzing the evolution of beta-endorphin post-translational processing events: studies on reptiles.
In many cartilaginous fishes, most ray-finned fishes, lungfishes, and amphibians, the post-translational processing of POMC includes the monobasic cleavage of beta-endorphin to yield an opioid that is eight to ten amino acids in length. The amino acid motif within the beta-endorphin sequence required for a monobasic cleavage event is -E-R-(S/G)-Q-. Mammals and birds lack this motif and as a result beta-endorphin(1-8) is a not an end-product in either group. Since both mammals and birds were derived from ancestors with reptilian origins, an analysis of beta-endorphin sequences from extant groups of reptiles should provide insights into the manner in which beta-endorphin post-translational processing mechanisms have evolved in amniotes. To this end a POMC cDNA was cloned from the pituitary of the turtle, Chrysemys scripta. The beta-endorphin sequence in this species was compared to other reptile beta-endorphin sequences (i.e., Chinese soft shell turtle and gecko) and to known bird and mammal sequences. This analysis indicated that either the loss of the arginine residue at the cleavage site (the two turtle species, chick, and human) or a substitution at the glutamine position in the consensus sequence (gecko and ostrich) would account for the loss of the monobasic cleavage reaction in that species. Since amphibians are capable of performing the beta-endorphin monobasic reaction, it would appear that the amino acid substitutions that eliminated this post-translational process event in reptilian-related tetrapods must have occurred in the ancestral amniotes.
Shoureshi
Pezhman
P
University of Denver, Department of Biological Sciences, 2190 E. Iliff, Olin Hall 102, Denver, CO 80210, USA.
Baron
Andrea
A
Szynskie
Laura
L
Dores
Robert M
RM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D019143:Evolution, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D011499:Protein Processing, Post-Translational / Q000235:genetics*; D012104:Reptiles / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014426:Turtles / Q000235:genetics / Q000378:metabolism; D001615:beta-Endorphin / Q000235:genetics* / Q000378:metabolism*
D001615:beta-Endorphin