Effect of experimental hypercholesterolaemia on K+ channel alpha-subunit mRNA levels in rabbit hearts.
We investigated the effect of dietary cholesterol on gene transcription of delayed rectifier (I(Kr) - ERG1 and I(Ks) - KvLQT1) and transient outward (I(to,fast) - Kv4.2 and Kv4.3) potassium channel subunits in rabbit hearts using real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR). While the level of Kv4.3 mRNA did not change, both Kv4.2 and ERG1 mRNAs were downregulated, whereas the level of KvLQT1 was increased in hypercholesterolaemic rabbits, indicating that hypercholesterolaemia altered ventricular K(+) channel alpha-subunit gene transcription.
Varga
Angelika
A
Department of Pharmacology and Pharmacotherapy, Medical and Health Science Center, University of Debrecen, Hungary. angelika.varga@king.pharmacol.dote.hu
Bagossi
Péter
P
Tözsér
József
J
Peitl
Barna
B
Szilvássy
Zoltán
Z
D000818:Animals; D002791:Cholesterol, Dietary / Q000008:administration & dosage*; D000072237:ERG1 Potassium Channel; D051638:Ether-A-Go-Go Potassium Channels; D015870:Gene Expression / Q000187:drug effects; D006937:Hypercholesterolemia / Q000209:etiology / Q000503:physiopathology; D051657:KCNQ1 Potassium Channel / Q000235:genetics; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D024642:Potassium Channels, Voltage-Gated / Q000235:genetics*; D021122:Protein Subunits / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051672:Shal Potassium Channels / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects
D002791:Cholesterol, Dietary; D000072237:ERG1 Potassium Channel; D051638:Ether-A-Go-Go Potassium Channels; D051657:KCNQ1 Potassium Channel; D024642:Potassium Channels, Voltage-Gated; D021122:Protein Subunits; D012333:RNA, Messenger; D051672:Shal Potassium Channels