Effects of either glucose or fructose and metabolic regulators on bovine embryo development and lipid accumulation in vitro.
Our objective was to determine if replacing glucose with fructose would decrease cytoplasmic lipid accumulation during culture of embryos with or without regulators of metabolism. In vitro-produced bovine zygotes were cultured 60 hr in chemically defined medium-1 (CDM-1) plus 0.5% BSA and 0.5 mM fructose or glucose in Experiment 1, and glucose in Experiment 2. In both experiments, 8-cell embryos were next cultured 135 hr in CDM-2 plus 2 mM fructose or glucose in factorial combination with five treatments: (Experiment 1: control, 10% fetal calf serum (FCS), 0.3 microM phenazine ethosulfate (PES), 30 microM dinitrophenol (DNP), and PES + DNP), and (Experiment 2: control, PES, PES + DNP, and 1 and 3 microg/ml cerulenin (C1 and C3)). Day 7.5 blastocysts were stained with Sudan Black B to quantify cytoplasmic lipid droplets as small (SD, <2 microm), medium (MD, 2-6 microm), or large (LD, >6 microm). Blastocyst rates per oocyte were 22% (Experiment 1) and 15% (Experiment 2) higher (P < 0.05) for fructose than glucose. For Experiment 1, numbers of MD were lower for PES, DNP, and PES + DNP than control and FCS (P < 0.05). LD were lower for PES and DNP than control, and higher for FCS than all other treatments (P < 0.05). For Experiment 2, MD were lower (P < 0.05) for PES, and PES + DNP than C1, C3, and control. For LD, PES was lower (P < 0.05) than control, C1, and C3, but not different from PES + DNP. The only effect of hexose on lipids was that fructose resulted in fewer MD (P < 0.01) in Experiment 2. In conclusion, fructose produced more blastocysts than glucose, and PES reduced lipid accumulation.
Barceló-Fimbres
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Animal Reproduction and Biotechnology Laboratory, Colorado State University, Fort Collins, Colorado, USA.
Seidel
G E
GE
D000818:Animals; D001755:Blastocyst / Q000187:drug effects*; D002417:Cattle; D002108:Ceruletide / Q000032:analysis / Q000494:pharmacology; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology*; D004140:Dinitrophenols / Q000032:analysis / Q000494:pharmacology; D046149:Embryo Culture Techniques; D047108:Embryonic Development / Q000187:drug effects*; D005632:Fructose / Q000032:analysis / Q000494:pharmacology*; D005947:Glucose / Q000032:analysis / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects*; D010619:Phenazines / Q000032:analysis / Q000494:pharmacology
D003470:Culture Media; D004140:Dinitrophenols; D010619:Phenazines; C028122:5-ethylphenazine; D005632:Fructose; D002108:Ceruletide; D005947:Glucose