title: Expression of glycolytic enzymes is increased in pancreatic cancerous tissues as evidenced by proteomic profiling by two-dimensional electrophoresis and liquid chromatography-mass spectrometry/mass spectrometry.
abstract: The prognosis of patients with pancreatic cancer is very poor because of late diagnosis and the lack of response to various therapies. We tried to identify proteins that might be available for early diagnosis and effective therapies by proteomic profiling of pancreatic cancer tissues. Pancreatic cancerous and paired non-cancerous tissues obtained from surgical resections or autopsies of 10 patients were analyzed by two-dimensional gel electrophoresis. The differential display showed 11 spots whose expression was increased in cancerous tissues compared with the paired non-cancerous tissues. The liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) system identified the spots as alpha-enolase, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), triosephosphate isomerase, transgelin, calmodulin, superoxide dismutase(Mn) mitochondrial precursor, glutathione S-transferase P, cyclophilin A, protein disulfide isomerase A3 precursor, and apolipoprotein A-I precursor. Two of the 11 spots were detected as GAPDH. We noticed that 4 of 11 spots were enzymes involved in glycolytic pathway. Increased glycolysis in cancer cells has been regarded as the effect of intratumoral hypoxia and is possibly associated with tumor invasion, metastasis or resistance to therapies. These glycolytic proteins and transgelin, were confirmed by Western blotting and immunohistochemistry.
authors.affiliation: Department of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan.
authors.forename: Kuniko
authors.initials: K
authors.lastname: Mikuriya
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kuramitsu
authors.forename: Shomei
authors.initials: S
authors.lastname: Ryozawa
authors.forename: Masanori
authors.initials: M
authors.lastname: Fujimoto
authors.forename: Sayaka
authors.initials: S
authors.lastname: Mori
authors.forename: Masaaki
authors.initials: M
authors.lastname: Oka
authors.forename: Kimikazu
authors.initials: K
authors.lastname: Hamano
authors.forename: Kiwamu
authors.initials: K
authors.lastname: Okita
authors.forename: Isao
authors.initials: I
authors.lastname: Sakaida
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Nakamura
mesh_terms: D000368:Aged; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006019:Glycolysis; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000175:diagnosis* / Q000201:enzymology*; D011506:Proteins / Q000032:analysis*; D040901:Proteomics; D015854:Up-Regulation
chemical_list: D011506:Proteins