Preparation and characterization of biodegradable anti-adhesive membrane for peritoneal wound healing.
Postoperative adhesions remain a significant complication of abdominal surgery although the wide variety of physical barriers has been developed to reduce the incidence of adhesion. In this study, the bilayered composite membrane formed by the association of a methoxy poly (ethylene glycol)-poly (L-lactide-co-glycolide) (mPEG-PLGA) film and a crosslinked collagen-hyaluronic acid (Col-HA) membrane with fibronectin (FN) coating was prepared for promoting wound healing and providing tissue adhesion resistance simultaneously. In vitro adhesion test revealed that fibroblasts attached better on Col-HA membrane compared to those on mPEG-PLGA film, PLGA film or Interceed (oxidized cellulose) while mPEG-PLGA film had the lowest cell adhesive property. In confocal microscopic observation, the actin filaments were significantly further polymerized when 50 or 100 microg/cm(3) fibronectin was incorporated on the COL-HA membranes. After 7-day culture, fibroblasts penetrated throughout the Col-HA-FN network and the cell density increased whereas very few cells were found attached on the surface of the mPEG-PLGA film. In vivo evaluation test showed that the composite membrane could remain during the critical period of peritoneal healing and did not provoke any inflammation or adverse tissue reaction.
Park
Si-Nae
SN
BK 21 Project Team of Nanobiomaterials for the Cell-based Implants, Yonsei University, Seodaemun-ku, Seoul, Korea.
Jang
Han Jeong
HJ
Choi
Yu Suk
YS
Cha
Jae Min
JM
Son
Seo Yeon
SY
Han
Seung Hun
SH
Kim
Jung Hyun
JH
Lee
Woo Jung
WJ
Suh
Hwal
H
D000818:Animals; D001672:Biocompatible Materials / Q000302:isolation & purification; D002478:Cells, Cultured; D003094:Collagen; D005260:Female; D005353:Fibronectins; D006801:Humans; D006820:Hyaluronic Acid; D066298:In Vitro Techniques; D008422:Materials Testing; D010537:Peritoneum / Q000601:surgery; D011091:Polyesters; D011092:Polyethylene Glycols; D011098:Polyglactin 910; D011183:Postoperative Complications / Q000517:prevention & control; D034421:Sus scrofa; D000267:Tissue Adhesions / Q000517:prevention & control*; D014945:Wound Healing
D001672:Biocompatible Materials; D005353:Fibronectins; D011091:Polyesters; C558447:methoxypolyethyleneglycol-poly(lactic-co-glycolic acid); D011098:Polyglactin 910; D011092:Polyethylene Glycols; D006820:Hyaluronic Acid; D003094:Collagen