title: [Adaptation of aerobic methylobacteria to dichloromethane degradation].
abstract: A shortening of the lag phase in dichloromethane (DCM) consumption was observed in the methylobacteria Methylopila helvetica DM6 and Albibacter methylovorans DM10 after prior growth on methanol with the presence of 1.5% NaCI. Neither heat nor acid stress accelerated methylobacterium adaptation to DCM consumption. Sodium azide (1 mM) and potassium cyanide (1 mM) inhibited consumption of DCM by these degraders but not by transconjugants Methylobacterium extorquens AM1, expressing DCM dehalogenase but unable to grow on DCM. This indicates that the degrader strains possess energy-dependent systems of transport of DCM or chloride anions produced during DCM dehalogenation. Inducible proteins were found in the membrane fraction of A. methylovorans DM10 cells adapted to DCM and elevated NaCl concentration.
authors.forename: M L
authors.initials: ML
authors.lastname: Torgonskaia
authors.forename: Iu E
authors.initials: IuE
authors.lastname: Firsova
authors.forename: N V
authors.initials: NV
authors.lastname: Doronina
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Trotsenko
mesh_terms: D000222:Adaptation, Physiological; D001426:Bacterial Proteins / Q000378:metabolism; D003227:Conjugation, Genetic; D008190:Lyases / Q000096:biosynthesis; D008565:Membrane Proteins / Q000378:metabolism; D000432:Methanol / Q000378:metabolism; D008752:Methylene Chloride / Q000378:metabolism*; D020580:Methylobacterium / Q000201:enzymology / Q000254:growth & development / Q000502:physiology*; D011190:Potassium Cyanide / Q000378:metabolism; D019810:Sodium Azide / Q000378:metabolism; D012965:Sodium Chloride / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D012965:Sodium Chloride; D008752:Methylene Chloride; D019810:Sodium Azide; D008190:Lyases; C045224:dichloromethane dehalogenase; D011190:Potassium Cyanide; D000432:Methanol