Separation of long DNA chains using a nonuniform electric field: a numerical study.
In the present study, we investigate the migration of DNA molecules through a microchannel using a series of electric traps controlled by an ac electric field. We describe the motion of DNA based on Brownian dynamics simulations of a bead-spring chain. The DNA chain captured by an electric field escapes due to thermal fluctuation. The mobility of the DNA chain was determined to depend on the chain length, the mobility of which sharply increases when the length of the chain exceeds a critical value that is strongly affected by the amplitude of the applied ac field. Thus we can optimize the separation selectivity of the channel for DNA molecules that is to be separated, without changing the structure of the channel. In addition, we present a phenomenological description for the relationship between the critical chain length and the strength of binding electric field.
Nagahiro
Shin-Ichiro
S
Department of Mechanical Engineering, Miyagi National College of Technology, Miyagi 981-1239, Japan. nagahiro@miyagi-ct.ac.jp
Kawano
Satoyuki
S
Kotera
Hidetoshi
H
D001703:Biophysics / Q000379:methods*; D003198:Computer Simulation; D004247:DNA / Q000737:chemistry*; D004560:Electricity; D004566:Electrodes; D004594:Electrophysiology; D015233:Models, Statistical; D008962:Models, Theoretical; D009690:Nucleic Acid Conformation
D004247:DNA