title: [Differentially expressed transcription factor-related genes in nasopharyngeal carcinoma and nasopharyngeal tissues].
abstract: OBJECTIVE
  To analyze the gene expression profiles of transcription factor-related genes in nasopharyngeal carcinoma (NPC) tissues and normal nasopharyngeal tissues using a cDNA microarray membrane for exploring the regulatory mechanism of differential gene express in NPC tissues.
  
  
  METHODS
  The total RNAs from 24 NPC tissues and 24 pooled normal nasopharyngeal tissues were reverse transcribed and labeled with alpha-(32)P-dCTP. The resultant cDNAs were hybridized to GF211 microarray, and the signals were analyzed by Pathway 4.0 software. RT-PCR was carried out to confirm the results.
  
  
  RESULTS
  Among the 1625 differentially expressed genes detected in NPC and nasopharyngeal tissues, 35 transcription factor-related genes were identified with either up- or down-regulation.
  
  
  CONCLUSIONS
  These differentially expressed transcription factor-related genes in NPC tissues might play a role in the regulation of NPC-related gene expression.
authors.affiliation: Oncology Institute, Key Lab of Molecular Tumor Pathology of Guangdong Province, Southern Medical University, Guangzhou 510515, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Wang
authors.forename: Li-Chun
authors.initials: LC
authors.lastname: Lu
authors.forename: Wei-Yi
authors.initials: WY
authors.lastname: Fang
authors.forename: Kai-Tai
authors.initials: KT
authors.lastname: Yao
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D050687:E2F1 Transcription Factor / Q000235:genetics; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D009687:Nuclear Proteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D050687:E2F1 Transcription Factor; D009687:Nuclear Proteins; D014157:Transcription Factors; C063552:transcription factor BTF3