Discovery of a natural thiamine adenine nucleotide.
Several important cofactors are adenine nucleotides with a vitamin as the catalytic moiety. Here, we report the discovery of the first adenine nucleotide containing vitamin B1: adenosine thiamine triphosphate (AThTP, 1), or thiaminylated ATP. We discovered AThTP in Escherichia coli and found that it accumulates specifically in response to carbon starvation, thereby acting as a signal rather than a cofactor. We detected smaller amounts in yeast and in plant and animal tissues.
Bettendorff
Lucien
L
Center for Cellular and Molecular Neurobiology, University of Liège, Avenue de l'Hôpital 1, B-4000 Liège, Belgium. l.bettendorff@ulg.ac.be
Wirtzfeld
Barbara
B
Makarchikov
Alexander F
AF
Mazzucchelli
Gabriel
G
Frédérich
Michel
M
Gigliobianco
Tiziana
T
Gangolf
Marjorie
M
De Pauw
Edwin
E
Angenot
Luc
L
Wins
Pierre
P
D000255:Adenosine Triphosphate / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D008968:Molecular Conformation; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D013838:Thiamine Triphosphate / Q000302:isolation & purification*
C519454:adenosine thiamine triphosphate; D013838:Thiamine Triphosphate; D000255:Adenosine Triphosphate