Quantitative detection of protein expression in single cells using droplet microfluidics.
We demonstrate that single cells can be controllably compartmentalized within aqueous microdroplets; using such an approach we perform high-throughput screening by detecting the expression of a fluorescent protein in individual cells with simultaneous measurement of droplet size and cell occupancy.
Huebner
A
A
Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK.
Srisa-Art
M
M
Holt
D
D
Abell
C
C
Hollfelder
F
F
deMello
A J
AJ
Edel
J B
JB
D005453:Fluorescence; D044085:Microfluidics / Q000379:methods*; D011506:Proteins / Q000032:analysis*
D011506:Proteins