title: Visualization of three-way comparisons of omics data.
abstract: BACKGROUND
  Density plot visualizations (also referred to as heat maps or color maps) are widely used in different fields including large-scale omics studies in biological sciences. However, the current color-codings limit the visualizations to single datasets or pairwise comparisons.
  
  
  RESULTS
  We propose a color-coding approach for the representation of three-way comparisons. The approach is based on the HSB (hue, saturation, brightness) color model. The three compared values are assigned specific hue values from the circular hue range (e.g. red, green, and blue). The hue value representing the three-way comparison is calculated according to the distribution of three compared values. If two of the values are identical and one is different, the resulting hue is set to the characteristic hue of the differing value. If all three compared values are different, the resulting hue is selected from a color gradient running between the hues of the two most distant values (as measured by the absolute value of their difference) according to the relative position of the third value between the two. The saturation of the color representing the three-way comparison reflects the amplitude (or extent) of the numerical difference between the two most distant values according to a scale of interest. The brightness is set to a maximum value by default but can be used to encode additional information about the three-way comparison.
  
  
  CONCLUSIONS
  We propose a novel color-coding approach for intuitive visualization of three-way comparisons of omics data.
authors.affiliation: Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan. richard.baran@gmail.com <richard.baran@gmail.com>
authors.forename: Richard
authors.initials: R
authors.lastname: Baran
authors.forename: Martin
authors.initials: M
authors.lastname: Robert
authors.forename: Makoto
authors.initials: M
authors.lastname: Suematsu
authors.forename: Tomoyoshi
authors.initials: T
authors.lastname: Soga
authors.forename: Masaru
authors.initials: M
authors.lastname: Tomita
mesh_terms: D000818:Animals; D003196:Computer Graphics; D003198:Computer Simulation; D030541:Databases, Genetic; D023281:Genomics / Q000379:methods*; D006801:Humans; D008954:Models, Biological; D020543:Proteome / Q000378:metabolism*; D012984:Software; D014584:User-Computer Interface
chemical_list: D020543:Proteome