title: Mechanochemical kinetics of transcription elongation.
abstract: The mechanochemical kinetics of transcription elongation was examined with a combination of theoretical and experimental approaches. The predictive power of a sequence-dependent thermal ratchet model for transcription elongation was tested by establishing model parameters based solely on measurements under chemical perturbations and then directly predicting responses under mechanical perturbations without additional model parameters. Agreement between predicted and measured force-velocity curves provides strong support for a simple mechanochemical coupling mechanism.
authors.affiliation: Department of Physics, Laboratory of Atomic and Solid State Physics, Cornell University, Ithaca, New York 14853, USA.
authors.forename: Lu
authors.initials: L
authors.lastname: Bai
authors.forename: Robert M
authors.initials: RM
authors.lastname: Fulbright
authors.forename: Michelle D
authors.initials: MD
authors.lastname: Wang
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry* / Q000378:metabolism*; D007700:Kinetics; D008957:Models, Genetic; D009711:Nucleotides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D009711:Nucleotides; D012333:RNA, Messenger; D004247:DNA; D012321:DNA-Directed RNA Polymerases