Identification of a Gene Sharing a Promoter and Peroxisome Proliferator-Response Elements With Acyl-CoA Oxidase Gene.
Many mammalian genes are clustered on the genomes, and hence the genes in the same cluster can be regulated through a common regulatory element. We indeed showed previously that the perilipin/PEX11alpha gene pair is transactivated tissue-selectively by PPARgamma and PPARalpha, respectively, through a common binding site. In the present study, we identified a gene, named GSPA, neighboring a canonical PPAR target, acyl-CoA oxidase (AOX) gene. GSPA expression was induced by a peroxisome proliferator, Wy14,643, in the liver of wild-type mice, but not PPARalpha-null mice. GSPA and AOX share the promoter and two peroxisome proliferator-response elements. GSPA mRNA was also found in the heart and skeletal muscle, as well as 3T3-L1 cells. GSPA encodes a protein of 161 amino acids that is enriched in 3T3-L1 cells. Even other gene pairs might be regulated through common sequence elements, and conversely it would be interesting how each gene is aptly regulated in clusters.
Akter
Mst Hasina
MH
Graduate School of Life Science, University of Hyogo, Hyogo 678-1297, Kamigori, Japan.
Razzaque
Md Abdur
MA
Yang
Liu
L
Fumoto
Toshio
T
Motojima
Kiyoto
K
Yamaguchi
Tomohiro
T
Hirose
Fumiko
F
Osumi
Takashi
T