title: Two functionally divergent UDP-Gal nucleotide sugar transporters participate in phosphoglycan synthesis in Leishmania major.
abstract: In the protozoan parasite Leishmania, abundant surface and secreted molecules, such as lipophosphoglycan (LPG) and proteophosphoglycans (PPGs), contain extensive galactose in the form of phosphoglycans (PGs) based on (Gal-Man-PO(4)) repeating units. PGs are synthesized in the parasite Golgi apparatus and require transport of cytoplasmic nucleotide sugar precursors to the Golgi lumen by nucleotide sugar transporters (NSTs). GDP-Man transport is mediated by the LPG2 gene product, and here we focused on transporters for UDP-Gal. Data base mining revealed 12 candidate NST genes in the L. major genome, including LPG2 as well as a candidate endoplasmic reticulum UDP-glucose transporter (HUT1L) and several pseudogenes. Gene knock-out studies established that two genes (LPG5A and LPG5B) encoded UDP-Gal NSTs. Although the single lpg5A(-) and lpg5B(-) mutants produced PGs, an lpg5A(-)/5B(-) double mutant was completely deficient. PG synthesis was restored in the lpg5A(-)/5B(-) mutant by heterologous expression of the human UDP-Gal transporter, and heterologous expression of LPG5A and LPG5B rescued the glycosylation defects of the mammalian Lec8 mutant, which is deficient in UDP-Gal uptake. Interestingly, the LPG5A and LPG5B functions overlap but are not equivalent, since the lpg5A(-) mutant showed a partial defect in LPG but not PPG phosphoglycosylation, whereas the lpg5B(-) mutant showed a partial defect in PPG but not LPG phosphoglycosylation. Identification of these key NSTs in Leishmania will facilitate the dissection of glycoconjugate synthesis and its role(s) in the parasite life cycle and further our understanding of NSTs generally.
authors.affiliation: Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110, USA.
authors.forename: Althea A
authors.initials: AA
authors.lastname: Capul
authors.forename: Tamara
authors.initials: T
authors.lastname: Barron
authors.forename: Deborah E
authors.initials: DE
authors.lastname: Dobson
authors.forename: Salvatore J
authors.initials: SJ
authors.lastname: Turco
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Beverley
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001692:Biological Transport; D015153:Blotting, Western; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D006028:Glycosphingolipids / Q000096:biosynthesis*; D006031:Glycosylation; D006056:Golgi Apparatus / Q000378:metabolism; D007700:Kinetics; D037102:Lectins / Q000378:metabolism; D018320:Leishmania major / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D033704:Nucleotide Transport Proteins / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D014531:Uridine Diphosphate Galactose / Q000378:metabolism*
chemical_list: D006028:Glycosphingolipids; D037102:Lectins; D008565:Membrane Proteins; D033704:Nucleotide Transport Proteins; D015800:Protozoan Proteins; C008290:lipophosphonoglycan; D014531:Uridine Diphosphate Galactose