Effect of aspirin on DNA damage induced by MMC in Drosophila.
In our previous paper, we found that aspirin suppressed the genotoxicity of mitomycin C (MMC) in a somatic mutation and recombination test (SMART) in Drosophila melanogaster. In order to reveal the mechanism of antigenotoxicity of aspirin, we evaluated the protective effects of aspirin against the genotoxicity of MMC with the DNA repair test in Drosophila melanogaster. Three types of treatment of aspirin were performed as co-, post- and pre-treatment. Aspirin co-treatment suppressed effectively the genotoxicity of MMC in a dose-dependent manner and the sex ratio at a dose of aspirin 10mg/bottle elevated from 0.01 (without aspirin) to 0.65 at sc z(1) w(+(TE)) mei-9(a) mei-41(D5)/-C(1)DX, y f [mei-9 mei-41, Rec(-) male.Rec(+) female] consists of DNA repair-deficient (Rec(-)) males and -proficient (Rec(+)) females. The antigenotoxic effect of aspirin on [mei-41, Rec(-) male.Rec(+) female] was similar to that on [mei-9, Rec(-) male.Rec(+) female]. But post- and pre-treatment by aspirin did not affect the genotoxicity of MMC on [mei-9 mei-41, Rec(-) male.Rec(+) female].
Niikawa
Miki
M
Department of Hygienics, Gifu Pharmaceutical University, 5-6-1 Mitahora-higashi, Gifu 502-8585, Japan. niikawam@tim.hi-ho.ne.jp
Nagase
Hisamitsu
H
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity*; D016587:Antimutagenic Agents / Q000494:pharmacology*; D001241:Aspirin / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects*; D004260:DNA Repair / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004331:Drosophila melanogaster; D005260:Female; D008297:Male; D016685:Mitomycin / Q000633:toxicity*; D009152:Mutagenicity Tests; D009154:Mutation
D000903:Antibiotics, Antineoplastic; D016587:Antimutagenic Agents; D016685:Mitomycin; D001241:Aspirin