Oral administration of a heat-killed cell preparation of Enterococcus faecalis strain EC-12 stimulates the gene expression of Na(+)-K(+)-2Cl- co-transporter 1 in rat ileal epithelial cells.
The effect of a heat-killed cell preparation of Enterococcus faecalis strain EC-12 (EC-12) on the gene expression of Na(+)-K(+)-2Cl(-) co-transporter 1 (NKCC1) in intestinal epithelial cells was evaluated by using rats. The NKCC1 gene in ileal epithelial cells was significantly up-regulated by the oral administration of EC-12. The results of this study suggest in vivo that EC-12 had the potential to stimulate intestinal NKCC1 expression.
Inoue
Ryo
R
Laboratory of Animal Science, Kyoto Prefectural University, Shimogamo, Kyoto, Japan.
Tsukahara
Takamitsu
T
Noda
Mamiko
M
Bukawa
Wakoto
W
Matsubara
Noritaka
N
Ushida
Kazunari
K
D000284:Administration, Oral; D000818:Animals; D013293:Enterococcus faecalis; D004847:Epithelial Cells / Q000378:metabolism*; D006358:Hot Temperature; D007082:Ileum / Q000378:metabolism*; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D028021:Sodium-Potassium-Chloride Symporters / Q000096:biosynthesis*; D064506:Solute Carrier Family 12, Member 2
C578879:Slc12a2 protein, rat; D028021:Sodium-Potassium-Chloride Symporters; D064506:Solute Carrier Family 12, Member 2