Characterization of poly(ethylene glycol)-modified bovine hemoglobin by capillary zone electrophoresis.
In this study capillary zone electrophoresis (CZE) was used to analyze the poly(ethylene glycol)-modified bovine hemoglobin(PEG-bHb). The results show that CZE separated the subunit of bovine hemoglobin based on the number of PEG conjugating to the protein surface, which makes it possible to evaluate the degree of modification of hemoglobin subunit; meanwhile, it also reflected the stability of PEG attaching to hemoglobin after incubating with hydroxylamine, which makes it successful to detect the distribution of attachment site and evaluate the stability of PEG on the surface of hemoglobin. As a simple, fast and accurate method, CZE is suitable to monitor the production procedures and quality control of the final products of the PEG-bHb.
Li
Xian-Zhong
XZ
Beijing Kaizheng Biotech Developing Ltd., Beijing, P.R. China.
Yang
Qin-Hong
QH
Yan
Jiu-Feng
JF
Xu
Ke
K
Liu
Jia
J
He
Xue-Ying
XY
Zhang
Xiao-Wei
XW
Liu
Qian
Q
D000818:Animals; D001802:Blood Substitutes / Q000138:chemical synthesis / Q000737:chemistry*; D002417:Cattle; D004355:Drug Stability; D019075:Electrophoresis, Capillary / Q000379:methods*; D006454:Hemoglobins / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D021122:Protein Subunits / Q000737:chemistry; D011786:Quality Control
D001802:Blood Substitutes; D006454:Hemoglobins; D021122:Protein Subunits; D011092:Polyethylene Glycols