title: A novel tetrameric lectin from Lycoris aurea with four mannose binding sites per monomer.
abstract: The mannose-binding agglutinin from bulbs of Lycoris aurea (LAA) agglutinates rabbit but not human erythrocytes. The molecular mass of the monomer in SDS/PAGE is 12 kDa while the apparent molecular mass in gel filtration is 48 kDa, indicating that LAA is a homotetramer. The full-length cDNA of LAA contains 683 bp with an open reading frame encoding a protomer of 162 amino-acid residues. Hydrophobic Cluster Analysis and molecular modeling of the 109-residue mature polypeptide suggested a similar secondary and tertiary structure to those of Narcissus pseudonarcissus agglutinin (NPA). Molecular docking revealed that, besides the three mannose-binding sites common among Amaryllidaceae lectins, LAA also contains a fourth unique mannose-binding site formed by a tryptophan cluster. The existence of four mannose-binding sites in each monomer of LAA is very unusual and has only been reported for NPA earlier.
authors.affiliation: College of Life Sciences, Sichuan University, Chengdu, PR China.
authors.forename: Jiwei
authors.initials: J
authors.lastname: Liu
authors.forename: Xiaochao
authors.initials: X
authors.lastname: Xu
authors.forename: Jinzhi
authors.initials: J
authors.lastname: Liu
authors.forename: Jan
authors.initials: J
authors.lastname: Balzarini
authors.forename: Yongtin
authors.initials: Y
authors.lastname: Luo
authors.forename: Yang
authors.initials: Y
authors.lastname: Kong
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Fang
authors.initials: F
authors.lastname: Chen
authors.forename: Els
authors.initials: E
authors.lastname: Van Damme
authors.forename: Jinku
authors.initials: J
authors.lastname: Bao
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002241:Carbohydrates / Q000032:analysis; D017124:Conserved Sequence; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics; D031426:Lycoris / Q000737:chemistry* / Q000235:genetics*; D008358:Mannose; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes / Q000737:chemistry; D037121:Plant Lectins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D016415:Sequence Alignment
chemical_list: D002241:Carbohydrates; D017931:DNA Primers; D018744:DNA, Plant; D046912:Multiprotein Complexes; D037121:Plant Lectins; D008358:Mannose