Site specific phosphorylation of cytochrome c oxidase subunits I, IVi1 and Vb in rabbit hearts subjected to ischemia/reperfusion.
We have mapped the sites of ischemia/reperfusion-induced phosphorylation of cytochrome c oxidase (CcO) subunits in rabbit hearts by using a combination of Blue Native gel/Tricine gel electrophoresis and nano-LC-MS/MS approaches. We used precursor ion scanning combined with neutral loss scanning and found that mature CcO subunit I was phosphorylated at tandem Ser115/Ser116 positions, subunit IVi1 at Thr52 and subunit Vb at Ser40. These sites are highly conserved in mammalian species. Molecular modeling suggests that phosphorylation sites of subunit I face the inter membrane space while those of subunits IVi1 and Vb face the matrix side.
Fang
Ji-Kang
JK
Laboratory of Biochemistry, The Department of Animal Biology, School of Veterinary Medicine, 3800 Spruce Street, University of Pennsylvania, Philadelphia, PA 19104, United States.
Prabu
Subbuswamy K
SK
Sepuri
Naresh B
NB
Raza
Haider
H
Anandatheerthavarada
Hindupur K
HK
Galati
Domenico
D
Spear
Joseph
J
Avadhani
Narayan G
NG
D000595:Amino Acid Sequence; D000818:Animals; D002853:Chromatography, Liquid; D003576:Electron Transport Complex IV / Q000737:chemistry* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005998:Glycine / Q000031:analogs & derivatives / Q000737:chemistry; D006321:Heart; D008969:Molecular Sequence Data; D015428:Myocardial Reperfusion Injury / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology*; D010766:Phosphorylation; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D011817:Rabbits; D012694:Serine / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D013912:Threonine / Q000378:metabolism
D021122:Protein Subunits; D013912:Threonine; D012694:Serine; D003576:Electron Transport Complex IV; D005998:Glycine; C100184:tricine