title: Epidermal growth factor (EGF) receptor endocytosis is accompanied by reorganization of microtubule system in HeLa cells.
abstract: Translocation of endosomes along microtubules (MTs) from the cell periphery toward the juxtranuclear region proximal to MTOC is well established. During this translocation the radial MT system is believed to retain its organization. Here we demonstrate that epidermal growth factor receptor (EGFR) endocytosis in HeLa cells is accompanied by dramatic remodeling of the MT system. Synchronized endocytosis was stimulated by warming the cells after EGF prebinding to EGFR on ice. Soon after that MTs were fully reestablished and EGFR was found in EE aligned along peripheral MTs. By the beginning of EE-to-LE sorting, the number of long MTs decreased and MTs appeared like an entangled meshwork of disorientated fragments and were partially depolymerized. Simultaneously, tubulin staining increased in juxtranuclear region, and at the time of LE-Lys interaction, enlarged EGFR-containing endosomes were localized there. Radial MTs were re-established when EGF-EGFR degradation started in lysosomes. In EGF absence, no alterations occurred upon MTs re-establishment. We conclude that MT remodeling is endocytosis-dependent.
authors.affiliation: Institute of Cytology of Russian Academy of Sciences, Tikhoretsky Ave, 4, 194064 St. Petersburg, Russia.
authors.forename: Marianna V
authors.initials: MV
authors.lastname: Kharchenko
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Aksyonov
authors.forename: Maria M
authors.initials: MM
authors.lastname: Melikova
authors.forename: Elena S
authors.initials: ES
authors.lastname: Kornilova
mesh_terms: D000818:Animals; D004705:Endocytosis; D004815:Epidermal Growth Factor / Q000378:metabolism; D066246:ErbB Receptors / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000378:metabolism*; D013347:Subcellular Fractions
chemical_list: D004815:Epidermal Growth Factor; D066246:ErbB Receptors