title: Profile of acute immune response in Chinese amphioxus upon Staphylococcus aureus and Vibrio parahaemolyticus infection.
abstract: Amphioxus is traditionally considered as the living invertebrate most closely related to vertebrate. However, no systematic study was performed about how the amphioxus defends against the microbial invasion. Here we reported a profile of gene transcription after Staphylococcus aureus (S.c) and Vibrio parahaemolyticus (V.p) challenged by suppression subtractive hybridization (SSH). When compared with mammals, amphioxus has the same acute immune defense genes (lectins, metalloproteinase, lysozymes and antimicrobial peptide, etc.) as well as a similar pattern and level of temporal gene expression. In contrast, amphioxus was demonstrated to have some novel acute immune response genes in response to the microbial challenge, such as apextrin and dermatopontin, which have a 3500-fold and 900-fold induction after the V.p infection, respectively, suggesting new functions in early immune system for these two genes. Our results reported for the first time a profile of primitive immune system defense against infection in protochordate.
authors.affiliation: State Key Laboratory of Biocontrol, College of Life Sciences, Sun Yat-sen University, Guangzhou 510275, People's Republic of China.
authors.forename: Gonghua
authors.initials: G
authors.lastname: Huang
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Han
authors.forename: Lifei
authors.initials: L
authors.lastname: Fan
authors.forename: Qinfen
authors.initials: Q
authors.lastname: Zhang
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Liu
authors.forename: Xuesong
authors.initials: X
authors.lastname: Yu
authors.forename: Lichun
authors.initials: L
authors.lastname: Zhang
authors.forename: Shangwu
authors.initials: S
authors.lastname: Chen
authors.forename: Meiling
authors.initials: M
authors.lastname: Dong
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Anlong
authors.initials: A
authors.lastname: Xu
mesh_terms: D000208:Acute Disease; D000818:Animals; D002816:Chordata, Nonvertebrate / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology; D020869:Gene Expression Profiling; D013203:Staphylococcal Infections / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology*; D014735:Vibrio Infections / Q000276:immunology*; D014736:Vibrio parahaemolyticus / Q000276:immunology*