[Biological identification on sub-cultivation cells of Schistosoma japonicum adult worms in vitro].
Cultivation of cells from 30-day old Schistosoma japonicum (S.j) adult worms showed that the growth features of the cells were semi-floating and accumulative. The survival rate of the primary cells, passage cells prior to the 5th generation and recovered cells was all up to 90%. Phases of cell division were observed during cultivation. Chromosome karyotype of the 5th generation cells possessed diploid feature of the blood-flukes (2n=8 in number). Ultrastructure of the 5th generation cells showed that four types of cells in normal morphology and three types of cells in abnormal morphology were both viewed. It is suggested that some of the cells from S.j adult worms were subcultured successfuly in the 1640-40 defined medium.
Liu
Wei
W
Department of Parasitology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Zeng
Tie-bing
TB
Zeng
Qing-ren
QR
Cai
Chun
C
Zhang
Zu-ping
ZP
Gong
Yan-fei
YF
Cai
Li-ting
LT
Zhang
Shun-ke
SK
Xu
Xi-ping
XP
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D007621:Karyotyping; D008940:Mitotic Index; D012549:Schistosoma japonicum / Q000166:cytology* / Q000235:genetics; D013997:Time Factors