Biotransformation of phenylpyruvic acid to phenyllactic acid by growing and resting cells of a Lactobacillus sp.
Phenyllactic acid (PLA) is a novel antimicrobial compound derived from phenylalanine (Phe). Lactobacillus sp. SK007, having high PLA-producing ability, was isolated from Chinese traditional pickles. When 6.1 mM phenylpyruvic acid (PPA) was used to replace Phe as substrate at the same concentration, PLA production increased 14-fold and the fermentation time decreased from 72 h to 24 h with growing cells. With resting cells, however, 6.8 mM PLA could be obtained as optimal yield using the following conditions: 12 mM PPA, 55 mM glucose, pH 7.5, 35 degrees C and 4 h.
Li
Xingfeng
X
The Key Laboratory of Food Science and Safety, Ministry of Education, Southern Yangtze University, Wuxi, China.
Jiang
Bo
B
Pan
Beilei
B
D001711:Biotransformation; D049109:Cell Proliferation; D007773:Lactates / Q000378:metabolism*; D007778:Lactobacillus / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification / Q000502:physiology*; D010667:Phenylpyruvic Acids / Q000378:metabolism*; D013045:Species Specificity
D007773:Lactates; D010667:Phenylpyruvic Acids; C017648:3-phenyllactic acid; C031606:phenylpyruvic acid