[Potassium channel blocker regulates the proliferation of prostatic epithelial cells in SD rats].
OBJECTIVE
To investigate the regulatory effect of potassium channel blocker (tetraethylammonium [TEA], aminopyridine [4-AP], glibenclamide [Glib]) on the proliferation of SD rat prostatic epithelial cells in vitro.


METHODS
The primary culture was prepared by collagenase dissociation of minced prostatic tissues. Cells were cultured in serum-free prostate epithelial cell growth media and identified by immunocytochemical studies. TEA and 4-AP at the concentration of 1, 5 and 10 mmol/L and Glib at the concentration of 10, 50 and 100 mol/L were added, and after 24, 48 and 72 hours of culturing, a cell column diagram was drawn and the cell number counted. The post-passage cell growth was observed by MTT assay and Hoechst33258 nucleus staining.


RESULTS
The cultured cells showed the typical morphological features of epithelia, with positive stain. MTT assay and Hoechst33258 staining showed that TEA, 4-AP and Glib at the increasing concentration effected different degrees of proliferation of prostatic epithelial cells after 24, 48 and 72 h (P < 0.01).


CONCLUSIONS
The potassium channel blocker is a direct physiological regulator of the proliferation of SD rat prostatic epithelial cells.
Zhou
Zheng-xing
ZX
Department of Urology, First Affiliated Hospital of Anhui Medical University, China.
Liang
Chao-zhao
CZ
Tang
Zhi-guo
ZG
Hao
Zong-yao
ZY
Guo
Qing-kui
QK
Hu
Yong
Y
Zhao
Jun
J
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects*; D008297:Male; D026902:Potassium Channel Blockers / Q000494:pharmacology*; D011467:Prostate / Q000166:cytology* / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley
D026902:Potassium Channel Blockers