title: Alveolar edema dispersion and alveolar protein permeability during high volume ventilation: effect of positive end-expiratory pressure.
abstract: OBJECTIVE
  To evaluate whether PEEP affects intrapulmonary alveolar edema liquid movement and alveolar permeability to proteins during high volume ventilation.
  
  
  METHODS
  Experimental study in an animal research laboratory.
  
  
  METHODS
  46 male Wistar rats.
  
  
  METHODS
  A (99m)Tc-labeled albumin solution was instilled in a distal airway to produce a zone of alveolar flooding. Conventional ventilation (CV) was applied for 30 min followed by various ventilation strategies for 3 h: CV, spontaneous breathing, and high volume ventilation with different PEEP levels (0, 6, and 8 cmH(2)O) and different tidal volumes. Dispersion of the instilled liquid and systemic leakage of (99m)Tc-albumin from the lungs were studied by scintigraphy.
  
  
  RESULTS
  The instillation protocol produced a zone of alveolar flooding that stayed localized during CV or spontaneous breathing. High volume ventilation dispersed alveolar liquid in the lungs. This dispersion was prevented by PEEP even when tidal volume was the same and thus end-inspiratory pressure higher. High volume ventilation resulted in the leakage of instilled (99m)Tc-albumin from the lungs. This increase in alveolar albumin permeability was reduced by PEEP. Albumin permeability was more affected by the amplitude of tidal excursions than by overall lung distension.
  
  
  CONCLUSIONS
  PEEP prevents the dispersion of alveolar edema liquid in the lungs and lessens the increase in alveolar albumin permeability due to high volume ventilation.
authors.affiliation: Centre de Recherche Bichat Beaujon CRB3, INSERM U773, site Bichat BP 416, Université Paris 7, Denis Diderot, 75018, Paris, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: de Prost
authors.forename: Damien
authors.initials: D
authors.lastname: Roux
authors.forename: Didier
authors.initials: D
authors.lastname: Dreyfuss
authors.forename: Jean-Damien
authors.initials: JD
authors.lastname: Ricard
authors.forename: Dominique
authors.initials: D
authors.lastname: Le Guludec
authors.forename: Georges
authors.initials: G
authors.lastname: Saumon
mesh_terms: D000818:Animals; D008297:Male; D011175:Positive-Pressure Respiration; D011650:Pulmonary Alveoli / Q000187:drug effects; D011654:Pulmonary Edema / Q000000981:diagnostic imaging / Q000209:etiology / Q000628:therapy*; D011877:Radionuclide Imaging; D051381:Rats; D017208:Rats, Wistar; D012121:Respiration, Artificial; D013668:Technetium Tc 99m Aggregated Albumin / Q000494:pharmacology; D013990:Tidal Volume
chemical_list: D013668:Technetium Tc 99m Aggregated Albumin