[Compare the effect of lipo- and normal prepared Sodium Morrhuate on ECV-304 cell line].
OBJECTIVE
To compare the effect of Sodium Morrhuate on ECV-304 between its lipo- and normal preparation.


METHODS
The ECV-304 cell line was supplemented with Sodium Morrhuate and lipo-Sodium Morrhuate in order, and the result on morphology (microscope, Giemsa Staining and electron microscope), cell activity (MTT), and flow cytometer between the two preparation were compared.


RESULTS
In normal preparation group, cell's edema occurred. Chromatin was like catkins. Tumefaction and degeneration of mitochondrion and endoplasmic reticulum appeared. In lipo-Sodium Morrhuate group, the membrane was creased and processus appeared. Chromatin aggregates to the membrane of nucleus was like crescent, and then broken. The apoptotic body was formed. MTT changes showed that the curve of the normal preparation group was steep and the change time was short relatively, which cues the vital cells decreased sharply. The curve of lipo-Sodium Morrhuate group was gentle and the change time was long relatively, which cues the vital cells decreased slowly. The flow cytometer showed that typical apoptosis peak appeared.


CONCLUSIONS
The normal preparation group shows an acute toxic effect on ECV-304 cell line, which result in a necrosis course, while lipo-Sodium Morrhuate shows a gradual releasing process, which may indicate a apoptosis course.
Tu
Jun-Bo
JB
Dept. of Oral and Maxillary Surgery, College of Stomatology, Xi'an Jiaotong University, Xi'an 710004, China.
Lan
Hai-Long
HL
Yang
Zhuang-Qun
ZQ
Zhang
Tie-Liang
TL
Song
Yong
Y
Xing
Zhe
Z
D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D006801:Humans; D009336:Necrosis; D012597:Sclerosing Solutions; D012976:Sodium Morrhuate
D012597:Sclerosing Solutions; D012976:Sodium Morrhuate