title: Enabling a community to dissect an organism: overview of the Neurospora functional genomics project.
abstract: A consortium of investigators is engaged in a functional genomics project centered on the filamentous fungus Neurospora, with an eye to opening up the functional genomic analysis of all the filamentous fungi. The overall goal of the four interdependent projects in this effort is to accomplish functional genomics, annotation, and expression analyses of Neurospora crassa, a filamentous fungus that is an established model for the assemblage of over 250,000 species of non yeast fungi. Building from the completely sequenced 43-Mb Neurospora genome, Project 1 is pursuing the systematic disruption of genes through targeted gene replacements, phenotypic analysis of mutant strains, and their distribution to the scientific community at large. Project 2, through a primary focus in Annotation and Bioinformatics, has developed a platform for electronically capturing community feedback and data about the existing annotation, while building and maintaining a database to capture and display information about phenotypes. Oligonucleotide-based microarrays created in Project 3 are being used to collect baseline expression data for the nearly 11,000 distinguishable transcripts in Neurospora under various conditions of growth and development, and eventually to begin to analyze the global effects of loss of novel genes in strains created by Project 1. cDNA libraries generated in Project 4 document the overall complexity of expressed sequences in Neurospora, including alternative splicing alternative promoters and antisense transcripts. In addition, these studies have driven the assembly of an SNP map presently populated by nearly 300 markers that will greatly accelerate the positional cloning of genes.
authors.affiliation: Department of Genetics, Dartmouth Medical School, Hanover, New Hampshire 03755, USA.
authors.forename: Jay C
authors.initials: JC
authors.lastname: Dunlap
authors.forename: Katherine A
authors.initials: KA
authors.lastname: Borkovich
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Henn
authors.forename: Gloria E
authors.initials: GE
authors.lastname: Turner
authors.forename: Matthew S
authors.initials: MS
authors.lastname: Sachs
authors.forename: N Louise
authors.initials: NL
authors.lastname: Glass
authors.forename: Kevin
authors.initials: K
authors.lastname: McCluskey
authors.forename: Michael
authors.initials: M
authors.lastname: Plamann
authors.forename: James E
authors.initials: JE
authors.lastname: Galagan
authors.forename: Bruce W
authors.initials: BW
authors.lastname: Birren
authors.forename: Richard L
authors.initials: RL
authors.lastname: Weiss
authors.forename: Jeffrey P
authors.initials: JP
authors.lastname: Townsend
authors.forename: Jennifer J
authors.initials: JJ
authors.lastname: Loros
authors.forename: Mary Anne
authors.initials: MA
authors.lastname: Nelson
authors.forename: Randy
authors.initials: R
authors.lastname: Lambreghts
authors.forename: Hildur V
authors.initials: HV
authors.lastname: Colot
authors.forename: Gyungsoon
authors.initials: G
authors.lastname: Park
authors.forename: Patrick
authors.initials: P
authors.lastname: Collopy
authors.forename: Carol
authors.initials: C
authors.lastname: Ringelberg
authors.forename: Christopher
authors.initials: C
authors.lastname: Crew
authors.forename: Liubov
authors.initials: L
authors.lastname: Litvinkova
authors.forename: Dave
authors.initials: D
authors.lastname: DeCaprio
authors.forename: Heather M
authors.initials: HM
authors.lastname: Hood
authors.forename: Susan
authors.initials: S
authors.lastname: Curilla
authors.forename: Mi
authors.initials: M
authors.lastname: Shi
authors.forename: Matthew
authors.initials: M
authors.lastname: Crawford
authors.forename: Michael
authors.initials: M
authors.lastname: Koerhsen
authors.forename: Phil
authors.initials: P
authors.lastname: Montgomery
authors.forename: Lisa
authors.initials: L
authors.lastname: Larson
authors.forename: Matthew
authors.initials: M
authors.lastname: Pearson
authors.forename: Takao
authors.initials: T
authors.lastname: Kasuga
authors.forename: Chaoguang
authors.initials: C
authors.lastname: Tian
authors.forename: Meray
authors.initials: M
authors.lastname: Baştürkmen
authors.forename: Lorena
authors.initials: L
authors.lastname: Altamirano
authors.forename: Junhuan
authors.initials: J
authors.lastname: Xu
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D004271:DNA, Fungal / Q000235:genetics; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015723:Gene Library; D005821:Genetic Techniques; D016681:Genome, Fungal; D023281:Genomics; D009154:Mutation; D009491:Neurospora / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide
chemical_list: D004271:DNA, Fungal