title: Transglutaminase 1 stabilizes beta-actin in endothelial cells correlating with a stabilization of intercellular junctions.
abstract: Microvascular endothelial monolayers from mouse myocardium become resistant to various barrier-compromising stimuli correlating with the expression of transglutaminase 1 (TGase1) and its translocation towards cellular junctions. In contrast, endothelial monolayers from mouse lung microvessels do not express TGase1 and remain sensitive to barrier-compromising stimuli corresponding to the known in vivo sensitivity of the lung microvasculature. Using the TGase-substrate 5-(biotinamido)-pentylamine, specific TGase inhibitors and RNAi, one target protein of TGase1 in endothelial cells was found to be beta-actin, suggesting that tissue-specific stabilization of the cortical actin filament network by intracellular TGase1 activity may play a role in controlling barrier properties of endothelial monolayers.
authors.affiliation: Department of Cellular Neurobionics, Institute of Biology II, RWTH Aachen, Aachen, and Institute of Anatomy and Cell Biology, University of Wurzburg, Wurzburg, Germany.
authors.forename: Werner
authors.initials: W
authors.lastname: Baumgartner
authors.forename: Agnes
authors.initials: A
authors.lastname: Weth
mesh_terms: D000199:Actins / Q000378:metabolism*; D000588:Amines / Q000378:metabolism; D000818:Animals; D001710:Biotin / Q000031:analogs & derivatives / Q000378:metabolism; D002103:Cadaverine / Q000031:analogs & derivatives / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002199:Capillary Permeability / Q000187:drug effects; D002460:Cell Line; D003331:Coronary Vessels / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007365:Intercellular Junctions / Q000187:drug effects / Q000378:metabolism*; D007476:Ionophores / Q000494:pharmacology; D008168:Lung / Q000098:blood supply*; D051379:Mice; D008833:Microcirculation / Q000166:cytology / Q000378:metabolism; D021381:Protein Transport; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D011503:Transglutaminases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
chemical_list: D000199:Actins; D000588:Amines; D004791:Enzyme Inhibitors; D007476:Ionophores; D034741:RNA, Small Interfering; C076022:5-(biotinamido)pentylamine; D000001:Calcimycin; D001710:Biotin; D011503:Transglutaminases; C105804:transglutaminase 1; C008542:monodansylcadaverine; D002103:Cadaverine; D002118:Calcium