T-cell antigen receptor assembly and cell surface expression is not affected by treatment with T-cell antigen receptor-alpha chain transmembrane Peptide.
A synthetic peptide termed core peptide (CP), which corresponds to a specific sequence of the TCR-alpha chain transmembrane domain, is known to inhibit IL-2 production in antigen stimulated T-cells. The molecular mechanism of the TCR inhibition is not known. This study examined the effects of CP on TCR subunit assembly and TCR cell surface expression in vitro. Co-transfection experiments between TCR-alpha and CD3-delta using COS-7 cells, and the interaction between TCR-alpha and the CD3 proteins in a T-cell line (2B4) were analysed after incubation with CP or its conjugates. Results indicate that CP co-precipitates with CD3-delta and CD3-epsilon in vitro, without any effect on TCR-alpha/CD3-delta dimerisation or TCR multisubunit assembly and cell surface expression.
Kurosaka
Nozomu
N
Department Orthopaedic Surgery, Fukuura Kanazawa-ku, Yokohama City University, Yokohama, Japan.
Bolte
Andrea
A
Ali
Marina
M
Manolios
Nicholas
N
D000818:Animals; D017252:CD3 Complex / Q000235:genetics / Q000378:metabolism; D019556:COS Cells; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism*; D002522:Chlorocebus aethiops; D019281:Dimerization; D015870:Gene Expression / Q000187:drug effects; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D010446:Peptide Fragments / Q000494:pharmacology*; D020836:Protein Structure, Quaternary; D021122:Protein Subunits; D021381:Protein Transport / Q000187:drug effects; D011948:Receptors, Antigen, T-Cell / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000737:chemistry* / Q000235:genetics; D014162:Transfection; D014407:Tumor Cells, Cultured
D017252:CD3 Complex; C472562:CD3delta antigen; C485468:Cd3e protein, mouse; D008565:Membrane Proteins; D010446:Peptide Fragments; D021122:Protein Subunits; D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta