title: Control of bioamine metabolism by 5-HT2B and alpha 1D autoreceptors through reactive oxygen species and tumor necrosis factor-alpha signaling in neuronal cells.
abstract: Homeostasis of the central nervous system relies on the proper integration of cell-signaling pathways recruited by a variety of neuronal and non-neuronal factors, with the aim of tightly controlling neurotransmitter metabolism, storage, and transport. We took advantage of the 1C11 neuroectodermal cell line, endowed with the capacity to selectively differentiate into serotonergic (1C11(5-HT)) or noradrenergic (1C11(NE)) neurons, to identify functional targets of serotonin (5-hydroxytryptamine [5-HT]) and norepinephrine (NE) autoreceptors possibly involved in the control of neuronal functions. We demonstrate that 5-HT(2B) and adreno alpha(1D) receptors are coupled to reactive oxygen species (ROS) production through NADPH oxidase activation in 1C11(5-HT) and 1C11(NE) neuronal cells, respectively. In the signaling cascade linking 5-HT(2B) receptors to NADPH oxidase, phospholipase A2-mediated arachidonic acid production is required for ROS synthesis. ROS, in turn, act as second message signals and control the activation of TACE (TNF-alpha converting enzyme), a member of a disintegrin and metalloproteinase family. 5-HT(2B) and alpha(1D) receptor stimulation triggers TACE-dependent TNF-alpha shedding in the surrounding milieu of 1C11(5-HT) and 1C11(NE) cells. In these cells, shed TNF-alpha triggers degradation of 5-HT and NE into 5-HIAA and MHPG, respectively. Finally, we observe that 5-HT(2B) and alpha(1D) receptor couplings to the NADPH oxidase-TACE cascade are strictly restricted to 1C11-derived progenies that have implemented a complete neuronal phenotype. Altogether, our data indicate that couplings of 5-HT(2B) and alpha(1D) autoreceptors to ROS and TNF-alpha signaling control neurotransmitter metabolism in 1C11-derived neuronal cells. Eventually, we might explain the origin of oxidative stress and high level of TNF-alpha in neurodegenerative diseases as a consequence of deviation of normal signaling pathways coupled to neurotransmitters.
authors.affiliation: Institut André Lwoff-Institut Pasteur, CNRS FRE 2937, Laboratoire Différenciation Cellulaire et Prions, 7 rue Guy Môquet, 94801 Villejuif Cedex, France. bschneid@vjf.cnrs.fr
authors.forename: Benoit
authors.initials: B
authors.lastname: Schneider
authors.forename: Mathéa
authors.initials: M
authors.lastname: Pietri
authors.forename: Sophie
authors.initials: S
authors.lastname: Mouillet-Richard
authors.forename: Myriam
authors.initials: M
authors.lastname: Ermonval
authors.forename: Vincent
authors.initials: V
authors.lastname: Mutel
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Launay
authors.forename: Odile
authors.initials: O
authors.lastname: Kellermann
mesh_terms: D000818:Animals; D001679:Biogenic Amines / Q000378:metabolism*; D002460:Cell Line; D008734:Methoxyhydroxyphenylglycol / Q000378:metabolism; D051379:Mice; D009474:Neurons / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D044403:Receptor, Serotonin, 5-HT2B / Q000502:physiology*; D018340:Receptors, Adrenergic, alpha-1 / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*
chemical_list: C547720:Adra1d protein, mouse; D001679:Biogenic Amines; D017382:Reactive Oxygen Species; D044403:Receptor, Serotonin, 5-HT2B; D018340:Receptors, Adrenergic, alpha-1; D014409:Tumor Necrosis Factor-alpha; D008734:Methoxyhydroxyphenylglycol