[Study on the early liver metastasis forecast of colorectal neoplasms].
OBJECTIVE
To obtain some effective objective markers used to predict the early liver metastasis of colorectal tumor, the relationship of liver metastasis of colorectal tumor with associate detection three markers such as CK20mRNA, CD44v6 and VEGF was studied.


METHODS
The expression of CK20mRNA in patrol venous blood from 30 colorectal cancer patients was detected by fluorescent quantitative RT-PCR, and the results of CD44v6 and VEGF in colorectal cancer tissue were determined by means of immunohistochemistry, and then compared with those in control groups.


RESULTS
The rate of positive expression of CK20mRNA in colorectal cancer patients' patrol venous blood was obviously superior to the level of benign pathological changes controls (P < 0.01), and significantly higher than that of normal controls (P < 0.01). The rate of positive expression of CD44v6 and VEGF in colorectal tumor tissue was distinctly superior to the level of benign pathological controls, and remarkable higher than that of normal controls (P < 0.01). The positive expression of liver metastasis was also clearly higher than that of no liver metastasis (P < 0.05). The rate of positive expression of CK20mRNA in patrol venous blood was evidently correlated to the expression of CD44v6 and VEGF in tumor tissue (r(1) = 0.933, r(2) = 0.906, P < 0.05). The results of associate detection of CK20mRNA, CD44v6 and VEGF were closely related to the incidence of liver metastasis.


CONCLUSIONS
If combined detecting these markers of CK20mRNA, CD44v6 and VEGF to forecast liver metastasis of colorectal tumor, the sensitivity and specialty of prediction will be improved, there were highly clinical values in predicting in early diagnosis liver metastasis of colorectal tumor.
Lin
Hui-Zhong
HZ
Department of General Surgery, Municipal Hospital of Qingdao City, Qingdao, China.
Chen
Lei
L
Zhou
Dong-Feng
DF
Hao
Lin-Hua
LH
Li
Xiao-Chuan
XC
Chang
Hong
H
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000473:pathology*; D042241:Early Diagnosis; D006801:Humans; D018960:Hyaluronan Receptors / Q000032:analysis; D007150:Immunohistochemistry; D053554:Keratin-20 / Q000097:blood / Q000235:genetics; D008113:Liver Neoplasms / Q000175:diagnosis* / Q000556:secondary*; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D042442:Vascular Endothelial Growth Factors / Q000032:analysis
D014408:Biomarkers, Tumor; D018960:Hyaluronan Receptors; D053554:Keratin-20; D012333:RNA, Messenger; D042442:Vascular Endothelial Growth Factors