title: [Relationship between transforming growth factor-alpha gene polymorphism and non-syndromic cleft lip with cleft palate].
abstract: OBJECTIVE
  To study the association of TGF-alpha gene polymorphism and non-syndromic cleft lip with cleft palate in Shandong province.
  
  
  METHODS
  Polymerase chain reaction combined with restrict enzyme digestion was used to detect the target gene variation in 98 patients with non-syndromic cleft lip with cleft palate and 101 healthy controls.
  
  
  RESULTS
  The C2 allele frequency in patients with non-syndromic cleft lip with cleft palate was significantly higher than that in healthy controls. The genotype frequency in patients with positive family history was significantly higher than that without positive family history.
  
  
  CONCLUSIONS
  TGF-alpha gene polymorphism is closely associated with non-syndromic cleft lip with cleft palate in Shandong, especially in patients with positive family history.
authors.affiliation: Dept. of Oral and Maxillofacial Surgery, The Second Hospital of Shandong University, Jinan 250033, China.
authors.forename: Kui-Feng
authors.initials: KF
authors.lastname: Yuan
authors.forename: Qing-Guo
authors.initials: QG
authors.lastname: Lai
authors.forename: De-Ren
authors.initials: DR
authors.lastname: Li
authors.forename: Zhong-Jun
authors.initials: ZJ
authors.lastname: Yang
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Zhou
mesh_terms: D002971:Cleft Lip; D002972:Cleft Palate; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D016211:Transforming Growth Factor alpha
chemical_list: D016211:Transforming Growth Factor alpha