title: [Age-dependent seroprevalence of Toscana virus in the Community of Madrid: 1993-1994 and 1999-2000].
abstract: OBJECTIVE
  The aim of this study was to assess the seroprevalence of Toscana virus (TOSV) in the Community of Madrid.
  
  
  METHODS
  Samples from two serosurveys obtained during 1993-1994 (2262 individuals) and 1999-2000 (1945 individuals) were studied. Samples were tested by ELISA for TOSV IgG detection.
  
  
  RESULTS
  The seroprevalence of TOSV IgG was significantly higher in 1993-1994 (7.2%; 95% CI 6.2-8.4) than in 1999-2000 (5.7%; 95% CI 4.7-6.9) (chi-square, p < 0.05). In both periods, the prevalence increased significantly with age.
  
  
  CONCLUSIONS
  These results confirm that TOSV has been circulating in the Community of Madrid over the last years.
authors.affiliation: Servicio de Microbiología Diagnóstica, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, España. fory@isciii.es
authors.forename: Fernando
authors.initials: F
authors.lastname: de Ory-Manchón
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Sanz-Moreno
authors.forename: Emiliano
authors.initials: E
authors.lastname: Aranguez-Ruiz
authors.forename: Rosa
authors.initials: R
authors.lastname: Ramírez-Fernández
mesh_terms: D000293:Adolescent; D000328:Adult; D017677:Age Distribution; D000368:Aged; D000369:Aged, 80 and over; D000914:Antibodies, Viral / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D008875:Middle Aged; D009017:Morbidity / Q000639:trends; D010217:Phlebotomus Fever / Q000453:epidemiology*; D012189:Retrospective Studies; D029301:Sandfly fever Naples virus / Q000276:immunology*; D016036:Seroepidemiologic Studies; D014505:Urban Population / Q000706:statistics & numerical data
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G