Microsatellite cross-amplification in coccolithophores: application in population diversity studies.
The development and isolation of microsatellites entails a significant input of time and money. Therefore there is an interest in using existing microsatellites on species from which markers have not yet been developed. Conservation of six previously identified microsatellite loci in the marine coccolithophorid species Emiliana huxleyi was found in a survey of two bloom forming coccolithophorid species--Gephyrocapsa oceanica and Coccolithus pelagicus. The number of alleles per locus varied from 1 to 8, and half of the microsatellite loci tested showed 4 or more alleles. The microsatellite markers used in this study may be applied to other coccolithophorid species for population analysis, eliminating the time-consuming, costly development of microsatellite markers for other coccolithophorid species.
Iglesias-Rodríguez
M Débora
MD
School of Ocean and Earth Science, National Oceanography Centre, Southampton, University of Southampton, European Way, Southampton, UK. dir@noc.soton.ac.uk
Probert
Ian
I
Batley
Jacqueline
J
D000483:Alleles; D048028:DNA, Algal / Q000378:metabolism; D056890:Eukaryota / Q000235:genetics*; D014644:Genetic Variation; D005828:Genetics, Population; D018895:Microsatellite Repeats
D048028:DNA, Algal