title: EGO, a novel, noncoding RNA gene, regulates eosinophil granule protein transcript expression.
abstract: Gene expression profiling of early eosinophil development shows increased transcript levels of proinflammatory cytokines, chemokines, transcription factors, and a novel gene, EGO (eosinophil granule ontogeny). EGO is nested within an intron of the inositol triphosphate receptor type 1 (ITPR1) gene and is conserved at the nucleotide level; however, the largest open reading frame (ORF) is 86 amino acids. Sucrose density gradients show that EGO is not associated with ribosomes and therefore is a noncoding RNA (ncRNA). EGO transcript levels rapidly increase following interleukin-5 (IL-5) stimulation of CD34(+) hematopoietic progenitors. EGO RNA also is highly expressed in human bone marrow and in mature eosinophils. RNA silencing of EGO results in decreased major basic protein (MBP) and eosinophil derived neurotoxin (EDN) mRNA expression in developing CD34(+) hematopoietic progenitors in vitro and in a CD34(+) cell line model. Therefore, EGO is a novel ncRNA gene expressed during eosinophil development and is necessary for normal MBP and EDN transcript expression.
authors.affiliation: School of Medicine, Department of Dermatology, University of Utah, Salt Lake City, Utah 84132, USA. lori.wagner@hsc.utah.edu
authors.forename: Lori A
authors.initials: LA
authors.lastname: Wagner
authors.forename: Clarissa J
authors.initials: CJ
authors.lastname: Christensen
authors.forename: Diane M
authors.initials: DM
authors.lastname: Dunn
authors.forename: Gerald J
authors.initials: GJ
authors.lastname: Spangrude
authors.forename: Ann
authors.initials: A
authors.lastname: Georgelas
authors.forename: Linda
authors.initials: L
authors.lastname: Kelley
authors.forename: M Sean
authors.initials: MS
authors.lastname: Esplin
authors.forename: Robert B
authors.initials: RB
authors.lastname: Weiss
authors.forename: Gerald J
authors.initials: GJ
authors.lastname: Gleich
mesh_terms: D002478:Cells, Cultured; D047091:Eosinophil Granule Proteins / Q000235:genetics*; D047093:Eosinophil Major Basic Protein / Q000235:genetics*; D047208:Eosinophil-Derived Neurotoxin / Q000235:genetics*; D004804:Eosinophils / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000235:genetics*; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D006801:Humans; D053496:Inositol 1,4,5-Trisphosphate Receptors / Q000235:genetics; D022661:RNA, Untranslated / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: D047091:Eosinophil Granule Proteins; D053496:Inositol 1,4,5-Trisphosphate Receptors; D022661:RNA, Untranslated; D047208:Eosinophil-Derived Neurotoxin; D047093:Eosinophil Major Basic Protein