[Multi-components quantitation by one marker new method for quality evaluation of Chinese herbal medicine].
OBJECTIVE
To establish a new quality evaluation method for Chinese herbal medicine, using one chemical reference substance to calculate multi-components simultaneously.


METHODS
Relative correction factors (RCF) of mutongsaponin B and C to saponin PJ1, a characteristic component as marker, were calcalated in The chromatographic conditions for determination of the three saponins in Caulis Akebiae akebiae as a research model. The contents of saponin PJ1 were determined by external standard method, and those of mutongsaponin B and C were calculated by saponin PJ1 and their RCF. The accuracy of the new method was evaluated by comparing the calculated contents with the determined contents.


RESULTS
The analysis procedures were validated. There has been no significant difference between the calculated contents and the determined contents, according to the angle cosine value, and the calcalated RCF have confidence value.


CONCLUSIONS
The method of multi-components quantitation by one marker has been verified in caulis akebiae and it is to be a new quality evaluation pattern for Chinese herbal medicines.
Wang
Zhi-min
ZM
Institute of Chinese Materia Medica, China Academy of Chinese Medicial Sciences, Beijing 100700, China.
Gao
Hui-min
HM
Fu
Xue-tao
XT
Wang
Wei-hao
WH
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000592:standards; D006801:Humans; D019684:Magnoliopsida / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis
C526388:2,3,23-trihydroxyurs-12-en-28-oic acid O-xylopyranosyl-1-3-rhamnopyranosyl-1-4-glucopyranosyl-1-6-glucopyranosyl ester; D004365:Drugs, Chinese Herbal; D012503:Saponins