title: Trapping hemoglobin in rigid matrices: fine tuning of oxygen binding properties by modulation of encapsulation protocols.
abstract: Encapsulation of hemoglobin in a biocompatible matrix is a potential strategy for obtaining blood substitutes. Such a system would retain most of the immunogenic and functional properties of the physiologically relevant oxygen carrier but would prevent protein extravasation and dimer/dimer dissociation. We applied this approach by entrapping hemoglobin in wet nanoporous silica gel, in the presence and absence of allosteric effectors. Silica gels, although not suitable for intravenous perfusion, are inert and optically transparent, thus allowing a full characterization of the functional and structural properties of encapsulated hemoglobin by spectroscopic techniques. Results indicate that hemoglobin molecules, entrapped using different protocols, exhibit an oxygen affinity that can be modulated between 12 and 140 torr. This tunability could be exploited to meet distinct clinical needs.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Parma, Parma, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Bruno
authors.forename: Luca
authors.initials: L
authors.lastname: Ronda
authors.forename: Stefano
authors.initials: S
authors.lastname: Bettati
authors.forename: Andrea
authors.initials: A
authors.lastname: Mozzarelli
mesh_terms: D001672:Biocompatible Materials; D002214:Capsules; D004337:Drug Carriers; D004339:Drug Compounding; D006454:Hemoglobins / Q000008:administration & dosage / Q000378:metabolism*; D006801:Humans; D010100:Oxygen / Q000378:metabolism*; D016062:Porosity; D011485:Protein Binding; D011487:Protein Conformation; D058428:Silica Gel; D012822:Silicon Dioxide
chemical_list: D001672:Biocompatible Materials; D002214:Capsules; D004337:Drug Carriers; D006454:Hemoglobins; D058428:Silica Gel; D012822:Silicon Dioxide; D010100:Oxygen