Quaternary ammonium zinc phthalocyanine: inhibiting telomerase by stabilizing G quadruplexes and inducing G-quadruplex structure transition and formation.
Water-soluble, octacationic zinc phthalocyanine (ZnPc) was found to be a very good G-quadruplex DNA stabilizer by using UV-melting studies and DNA polymerase stop assays, and a potent telomerase inhibitor by using the telomeric repeat amplification protocol (TRAP) assay. The compound's DNA-binding properties were also studied by surface plasmon resonance (SPR). Furthermore, CD experiments demonstrated that ZnPc could induce intramolecular G-quadruplex structure transition from the antiparallel to parallel form. More importantly, ZnPc was found to induce parallel structure formation in cation-deficient conditions. The stability of the induced structure was determined with CD melting assays.
Ren
Lige
L
College of Chemistry and Molecular Sciences, Wuhan University, Hongshan, Luo Jia Shan, Hubei, Wuhan 430072, P.R. China.
Zhang
Anming
A
Huang
Jing
J
Wang
Ping
P
Weng
Xiaocheng
X
Zhang
Lixia
L
Liang
Feng
F
Tan
Zheng
Z
Zhou
Xiang
X
D001483:Base Sequence; D002942:Circular Dichroism; D017931:DNA Primers; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007211:Indoles / Q000494:pharmacology*; D054833:Isoindoles; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000494:pharmacology*; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D020349:Surface Plasmon Resonance; D019098:Telomerase / Q000037:antagonists & inhibitors*; D014466:Ultraviolet Rays; D017967:Zinc Compounds
D017931:DNA Primers; D004791:Enzyme Inhibitors; D007211:Indoles; D054833:Isoindoles; D009942:Organometallic Compounds; D000644:Quaternary Ammonium Compounds; D017967:Zinc Compounds; C052159:Zn(II)-phthalocyanine; D019098:Telomerase