Quantification of magnetic susceptibility in several strains of Bacillus spores: implications for separation and detection.
Three strains of Bacillus: Bacillus atrophaeus (formally Bacillus globigii), Bacillus thuringiensis, and Bacillus cereus were tested for their intrinsic magnetic susceptibility. All three strains when sporulated demonstrated significant magnetic susceptibility using an instrument referred to as Cell Tracking Velocimetry. Energy dispersive spectroscopy also confirmed the presence of paramagnetic elements, Fe and Mn, in the spore form of the bacteria. It was demonstrated that this magnetic susceptibility is sufficient to separate and deposit these spores on glass slides in a magnetic deposition system. These results indicate the potential to separate spores with intrinsic magnetic susceptibility directly out of water or air samples.
Melnik
Kristie
K
Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Ave, Columbus, Ohio 43210, USA.
Sun
Jianxin
J
Fleischman
Aaron
A
Roy
Shuvo
S
Zborowski
Maciej
M
Chalmers
Jeffrey J
JJ
D001407:Bacillus / Q000145:classification / Q000302:isolation & purification* / Q000502:physiology / Q000528:radiation effects*; D002469:Cell Separation / Q000379:methods*; D004586:Electrophoresis / Q000379:methods; D008280:Magnetics; D013045:Species Specificity; D013171:Spores, Bacterial / Q000145:classification / Q000302:isolation & purification / Q000502:physiology / Q000528:radiation effects