Investigation for analytical procedure for determination of trace metallic ions in simulated body fluids by inductively coupled plasma atomic emission spectrometry (ICP-AES).
Influences of matrix elements and high viscosity in three kind of simulated body fluids (SBFs) on determination of trace metallic elements (Co, Cr, Ni, Al and V) by inductively coupled plasma atomic emission spectrometry (ICP-AES) were investigated. In addition, decreases of these effects were attempted by H(2)SO(4) fume treatment. Calibration lines of the elements were constructed by the standard solutions made of elemental solutions and HCl or the SBFs. Gradients of calibration lines constructed by the each standard solution were different. Therefore, for accurate determination, calibration curve must be constructed by the elemental standard solution and the analytical solution. Limit of detection (LOD) of each element in the solutions was measured by a blank test. Although LODs of microg [Symbol: see text] L(-1) (ppb) order were nominal instrumental data, because of influences of the matrix elements and the high viscosity, the measured LODs of the elements in the SBFs were higher than those. However, the LODs were lowered by employing the H(2)SO(4)-fume treatment and approached to the nominal instrumental data. Therefore, H(2)SO(4)-fume treatment is extremely effective treatment in order to reduce the influences.
Ohtsu
Naofumi
N
Institute for Materials Research, Tohoku University, Sendai, Japan. nohtsu@imr.tokoku.ac.jp
Ashino
Tetsuya
T
Kimura
Hisamichi
H
Takada
Kunio
K
Hanawa
Takao
T
D001672:Biocompatible Materials / Q000032:analysis; D001826:Body Fluids / Q000737:chemistry*; D003343:Corrosion; D066298:In Vitro Techniques; D008422:Materials Testing; D013054:Spectrophotometry, Atomic / Q000379:methods*; D014131:Trace Elements / Q000032:analysis*; D014783:Viscosity
D001672:Biocompatible Materials; D014131:Trace Elements