Efficient RNA polyuridylation by noncanonical poly(A) polymerases.
Nuclear poly(A) polymerase (PAP) polyadenylates nascent mRNAs, promoting their nuclear export, stability, and translation, while the related cytoplasmic polymerase GLD-2 activates translation of deadenylated mRNAs. Here we characterize the biochemical activity of fission yeast Schizosaccharomyces pombe Cid1, a putative cytoplasmic PAP implicated in cell cycle checkpoint controls. Surprisingly, Cid1 has robust poly(U) polymerase activity in vitro, especially when isolated in native multiprotein complexes. Furthermore, we found that upon S-phase arrest, the 3' ends of actin mRNAs were posttranscriptionally uridylated in a Cid1-dependent manner. Finally, Hs2 (ZCCHC6), a human ortholog of Cid1, shows similar activity. These data suggest that uridylation of mRNA forms the basis of an evolutionarily conserved mechanism of gene regulation.
Rissland
Olivia S
OS
Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom.
Mikulasova
Andrea
A
Norbury
Chris J
CJ
D000199:Actins / Q000235:genetics / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D001483:Base Sequence; D002453:Cell Cycle / Q000502:physiology; D006801:Humans; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes; D009713:Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism*; D011061:Poly A / Q000378:metabolism*; D011062:Polynucleotide Adenylyltransferase / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*; D014542:Uridine Monophosphate / Q000378:metabolism*
D000199:Actins; D009097:Multienzyme Complexes; D012333:RNA, Messenger; D011994:Recombinant Proteins; D029702:Schizosaccharomyces pombe Proteins; D011061:Poly A; D000255:Adenosine Triphosphate; D014542:Uridine Monophosphate; D009713:Nucleotidyltransferases; C520554:Cid1 protein, S pombe; D011062:Polynucleotide Adenylyltransferase