[Development of O-serogroup serodiagnosis for patients with hemolytic uremic syndrome by Ec-LPS array].
We extracted lipopolysaccharides from 58 O-serogroup strains of Escherichia coli with phenol-water for use as antigens for an Ec-LPS array. The Ec-LPS array was made by dot-blotting of E. coli LPS on PVDF membrane. Commercial anti-E. coli O-serogroup antisera reacted with homologous O-serogroup LPS in Ec-LPS arrays. Convalescent sera of 6 patients with hemolytic uremic syndrome reacted strongly with O157 LPS when IgM and IgA antibodies in patient sera analyzed by Ec-LPS arrays. When IgG antibody was analyzed in this array, it was difficult to diagnose the O-serogroup because of the reactivity of patient sera against many O-serogroup LPS. These results match those by ELISA and western blotting. Compared to these serological techniques, Ec-LPS array appears superior to ELISA and western blotting in cost performance, time performance, and technical complexity.
Amano
Ken-ichi
K
Bioscience Education Research Center, Akita University.
Yatsuyanagi
Jun
J
Saito
Shioko
S
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D004926:Escherichia coli / Q000276:immunology*; D004927:Escherichia coli Infections / Q000175:diagnosis*; D019453:Escherichia coli O157 / Q000276:immunology; D006463:Hemolytic-Uremic Syndrome / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D019081:O Antigens / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology; D012698:Serologic Tests
D019081:O Antigens; D011135:Polysaccharides, Bacterial