Specific antibody detection in serum, urine and saliva samples for the diagnosis of cystic echinococcosis.
Serum, saliva and urine samples of 25 clinically and radiologically diagnosed cystic echinoccosis (CE) patients, 25 clinically suspected cases of CE, 15 other parasitic disease controls and 25 healthy controls were evaluated for anti-hydatid antibody response by ELISA. The sensitivity of serum, saliva and urine was found to be 72, 56 and 84%, respectively, while specificity was 76% in all the samples. Urine showed significantly higher (p<0.05) sensitivity than that of saliva samples but not significantly higher (p>0.05) than that of serum samples. There was no significant difference in the immune response of patients with hepatic versus extrahepatic cysts and single versus multiple cysts. Thus, biological fluid like urine may be used as an alternative or as an adjunct to serum samples by virtue of its non-invasive, easy collection and similar sensitivity and specificity.
Sunita
T
T
Department of Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh 160012, India.
Dubey
M L
ML
Khurana
Sumeeta
S
Malla
Nancy
N
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood* / Q000302:isolation & purification / Q000652:urine; D016022:Case-Control Studies; D002648:Child; D003937:Diagnosis, Differential; D004443:Echinococcosis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D048209:Echinococcus granulosus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010272:Parasitic Diseases / Q000097:blood / Q000175:diagnosis* / Q000652:urine; D012463:Saliva / Q000469:parasitology
D000909:Antibodies, Helminth