Annexin B1 at the host-parasite interface of the Taenia solium cysticercus: Secreted and associated with inflammatory reaction.
Annexin B1 is a novel member of annexin family firstly cloned by immunological screening a Taenia solium cysticercus library. To investigate the histological distribution and physiological role(s) of this protein, we first prepared a specific monoclonal antibody against annexin B1. Western blot analysis indicated that annexin B1 could be detected in cystic fluid of T. solium cysticercus and sera of pigs/humans with cysticercosis. Thus, annexin B1 might belong to the secreted members of annexins. Immunohistochemical analysis revealed that annexin B1 was mainly present in the tegument of bladders, but not in the scolex and neck; it was also detected in the surrounding host-derived layer with granulomatous infiltration. Together with previous, the presented data suggested that the protein inhibited mammalian PLA2 in vitro, and might down regulate host inflammatory responses.
Gao
Yuan-Jian
YJ
Department of Medical Genetics, The Second Military Medical University, Xiangyin Road 800, Shanghai 200433, China.
Yan
Hong-Li
HL
Ding
Fei-Xiang
FX
Lu
Yi-Ming
YM
Sun
Shu-Han
SH
D000818:Animals; D017302:Annexins / Q000378:metabolism / Q000502:physiology*; D003551:Cysticercosis / Q000378:metabolism / Q000469:parasitology* / Q000473:pathology; D015801:Helminth Proteins / Q000378:metabolism / Q000502:physiology*; D006790:Host-Parasite Interactions / Q000502:physiology*; D006801:Humans; D007249:Inflammation / Q000469:parasitology; D013552:Swine
D017302:Annexins; D015801:Helminth Proteins; C449006:annexin B1, Taenia solium