Arvanil and anandamide up-regulate CD36 expression in human peripheral blood mononuclear cells.
In this study we analysed the regulation of gene expression by arvanil and anandamide in human peripheral blood mononuclear cells (PBMCs) to clarify their immunosuppressive properties. PBMCs were activated, leading to CD36 down regulation, that was normalized by arvanil and anandamide. We used microarray technology to identify a regulatory pattern associated with cell proliferation in the presence of both substances. CD3-CD28 stimulated PBMCs showed a pattern of up-regulated and down-regulated genes after treatment with these substances. We selected and analysed several genes chosen by their function in the regulation of cell proliferation. We showed a transcriptional control of the CD36 gene by arvanil and anandamide associated with an increased protein expression, thus suggesting a possible role of CD36 in anandamide and arvanil anti-inflammatory pattern.
Malfitano
Anna Maria
AM
Dipartimento di Scienze Farmaceutiche, Università di Salerno, Fisciano (SA), Italy.
Toruner
Gokce Altay
GA
Gazzerro
Patrizia
P
Laezza
Chiara
C
Husain
Seema
S
Eletto
Davide
D
Orlando
Pierangelo
P
De Petrocellis
Luciano
L
Terskiy
Alexandra
A
Schwalb
Marvin
M
Vitale
Emilia
E
Bifulco
Maurizio
M
D001095:Arachidonic Acids / Q000276:immunology / Q000494:pharmacology*; D018106:CD28 Antigens / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology; D018955:CD36 Antigens / Q000096:biosynthesis* / Q000097:blood / Q000276:immunology; D002211:Capsaicin / Q000031:analogs & derivatives* / Q000276:immunology / Q000494:pharmacology; D063388:Endocannabinoids; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D053284:Polyunsaturated Alkamides / Q000276:immunology / Q000494:pharmacology*; D015854:Up-Regulation / Q000187:drug effects
D001095:Arachidonic Acids; D018106:CD28 Antigens; D017252:CD3 Complex; D018955:CD36 Antigens; D063388:Endocannabinoids; D053284:Polyunsaturated Alkamides; C121153:arvanil; D002211:Capsaicin; C078814:anandamide