Study of morphology and rigidity of neutrophilic granulocyte membrane in the real time mode by scanning probe microscopy.
The rigidity of neutrophilic granulocyte membrane was determined: 2.1+/-0.7 kPa. Scanning in the contact mode did not modify cell morphology, while spectroscopy caused nonspecific swelling reaction and a 3-fold increase in cell volume. Spectroscopy had no effect on rigidity of neutrophilic granulocyte membrane.
Pleskova
S N
SN
Research and Educational Center "Physics of Solid Body Nanostructures", N. I. Lobachevsky Nizhny Novgorod State University. plsn@mail.ru
Gushchina
Yu Yu
YY
Zvonkova
M B
MB
Khomutov
A E
AE
D002462:Cell Membrane / Q000648:ultrastructure*; D004548:Elasticity; D006801:Humans; D020527:Microscopy, Scanning Probe / Q000379:methods*; D009504:Neutrophils / Q000166:cytology*; D013057:Spectrum Analysis