Flow cytometric analysis of CD4+ T cell receptor zeta chain deficiency in patients with systemic lupus erythematosus.
OBJECTIVE
It has been reported that a dysfunction of T lymphocytes can be responsible for alteration in immune system in patients with systemic lupus erythematosus (SLE).


METHODS
Using flow cytometric analysis, we determined the abnormalities of T cell receptor zeta (TCR zeta) chain contents in CD4+ T cells of SLE patients.


RESULTS
We observed a decrease in mean fluorescence intensity of TCR zeta in CD4+ T cells of patients with SLE. The multiple analysis did not show a correlation between gender, age, disease specific manifestation, treatment, duration and TCR zeta mean fluorescence intensity in CD4+ T cells.


CONCLUSIONS
High prevalence of TCR zeta chain deficiency in CD4+ T cells confirms the significance of this signaling molecule in SLE pathogenesis.
Duriagin
S
S
Department of Biochemistry and Molecular Biology, Poznań University of Medical Sciences, Poland.
Ofori
H
H
Jagodziński
P P
PP
D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology* / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000172:deficiency* / Q000276:immunology; D008875:Middle Aged; D011948:Receptors, Antigen, T-Cell / Q000172:deficiency / Q000276:immunology* / Q000378:metabolism
D008565:Membrane Proteins; D011948:Receptors, Antigen, T-Cell; C054979:antigen T cell receptor, zeta chain