Characteristics of novel myeloid precursor cell line, PC-MDS, established from a bone marrow of the patient with therapy-related myelodysplastic syndrome.
We report on characteristics of the first human cell line, PC-MDS, derived from a bone marrow of a patient with therapy-related myelodysplastic syndrome (t-MDS) who had no overt post-MDS leukemia. Classic cytology analyses, immunophenotyping, cytogenetic and molecular genetic procedures were used for characterization of the cell line. PC-MDS cells are positive for the expression of CD13, CD15, CD30, CD33, and CD45 antigen. Positive cytochemical staining and immunophenotype analyses indicated that PC-MDS cells have some characteristics of the early myeloid precursor cell. The karyotype analysis of PC-MDS cell line revealed various numerical and structural changes including those typically associated with t-MDS: del(5)(q13)[7], der(5)t(5;11)(p11;q11)[13], -7[6], del(7)(q31)[2], +20[3], -20[4]. Evaluation of methylation status in a promoter region of p15, p16 and MGMT genes showed biallelic hypermethylation pattern of 5' promoter region only in MGMT gene. PC-MDS is the first t-MDS derived cell line, and based on its immunological, cytogenetic and molecular characterization could be a new tool in evaluation of complex biology of MDS and a model for methylation studies.
Bogdanović
Gordana
G
Institute of Oncology Sremska Kamenica, Institutski put 4, Sremska Kamenica, Serbia. bogdanovic.gordana@onko.onk.ns.ac.yu
Jurisić
Vladimir
V
Kraguljac
Nada
N
Mrdjanović
Jasminka
J
Jakimov
Dimitar
D
Krtolica
Koviljka
K
Krajnović
Milena
M
Magić
Zvonko
Z
Stojiljković
Bratislav
B
Andrijević
Ljiljana
L
Srdić
Tatjana
T
Baltić
Mirjana
M
Popović
Stevan
S
D000328:Adult; D000970:Antineoplastic Agents / Q000627:therapeutic use; D001853:Bone Marrow / Q000473:pathology*; D050762:Cyclin-Dependent Kinase Inhibitor p15 / Q000235:genetics / Q000378:metabolism; D019175:DNA Methylation; D015254:DNA Modification Methylases / Q000235:genetics; D045643:DNA Repair Enzymes / Q000235:genetics; D019672:Genes, T-Cell Receptor / Q000235:genetics; D019942:Genes, p16; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D009190:Myelodysplastic Syndromes / Q000473:pathology*; D023461:Myeloid Progenitor Cells / Q000473:pathology*; D016133:Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins / Q000235:genetics
D000970:Antineoplastic Agents; D050762:Cyclin-Dependent Kinase Inhibitor p15; D007135:Immunoglobulin Variable Region; D025521:Tumor Suppressor Proteins; D015254:DNA Modification Methylases; C515491:MGMT protein, human; D045643:DNA Repair Enzymes