Bioactive surface modification of mica and poly(dimethylsiloxane) with hydrophobins for protein immobilization.
Bioactive surfaces with appropriate hydrophilicity for protein immobilization can be achieved by hydrophobin II (HFBI) self-assembly on mica and polydimethylsiloxane (PDMS) surfaces. X-ray photoelectron spectroscopy and water contact angle measurements illustrated that the surface wettability can be changed from superhydrophobic (PDMS) or superhydrophilic (mica) to moderately hydrophilic, which is suitable for protein (chicken IgG) immobilization on both substrate surfaces. The results suggest that HFBI assembly, one kind of hydrophobin from Trichoderma reesei, may be a versatile and convenient method for the immobilization of biomolecules on diverse substrates, which may have potential applications in biosensors, immunoassays, and microfluidic networks.
Qin
Ming
M
The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin 300071, PR China.
Wang
Li-Kai
LK
Feng
Xi-Zeng
XZ
Yang
Yan-Lian
YL
Wang
Rui
R
Wang
Chen
C
Yu
Lei
L
Shao
Bin
B
Qiao
Ming-Qiang
MQ
D000538:Aluminum Silicates / Q000737:chemistry*; D000818:Animals; D002627:Chemistry, Physical / Q000379:methods; D002645:Chickens; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000737:chemistry; D044085:Microfluidics; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D011506:Proteins / Q000737:chemistry*; D012828:Silicones / Q000737:chemistry*; D013052:Spectrometry, X-Ray Emission; D013499:Surface Properties; D014242:Trichoderma / Q000378:metabolism; D014867:Water / Q000737:chemistry
D000538:Aluminum Silicates; D004129:Dimethylpolysiloxanes; D007074:Immunoglobulin G; D011506:Proteins; D012828:Silicones; D014867:Water; C013830:baysilon; C011934:mica