[Differentially expressed transcription factor-related genes in nasopharyngeal carcinoma and nasopharyngeal tissues].
OBJECTIVE
To analyze the gene expression profiles of transcription factor-related genes in nasopharyngeal carcinoma (NPC) tissues and normal nasopharyngeal tissues using a cDNA microarray membrane for exploring the regulatory mechanism of differential gene express in NPC tissues.


METHODS
The total RNAs from 24 NPC tissues and 24 pooled normal nasopharyngeal tissues were reverse transcribed and labeled with alpha-(32)P-dCTP. The resultant cDNAs were hybridized to GF211 microarray, and the signals were analyzed by Pathway 4.0 software. RT-PCR was carried out to confirm the results.


RESULTS
Among the 1625 differentially expressed genes detected in NPC and nasopharyngeal tissues, 35 transcription factor-related genes were identified with either up- or down-regulation.


CONCLUSIONS
These differentially expressed transcription factor-related genes in NPC tissues might play a role in the regulation of NPC-related gene expression.
Wang
Shuang
S
Oncology Institute, Key Lab of Molecular Tumor Pathology of Guangdong Province, Southern Medical University, Guangzhou 510515, China.
Lu
Li-Chun
LC
Fang
Wei-Yi
WY
Yao
Kai-Tai
KT
D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D050687:E2F1 Transcription Factor / Q000235:genetics; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D009687:Nuclear Proteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics*; D014407:Tumor Cells, Cultured
D050687:E2F1 Transcription Factor; D009687:Nuclear Proteins; D014157:Transcription Factors; C063552:transcription factor BTF3