Advances in neuromembrane proteomics: efforts towards a comprehensive analysis of membrane proteins in the brain.
Proteomic investigation of normal and diseased brain states has the potential to reveal novel molecular therapeutic and diagnostic targets for a multitude of pathological central nervous system conditions. Due to their unique properties, integral membrane proteins are likely to play a central role in the aetiology of these disorders. These properties, however, have prevented comprehensive analysis of this important class of proteins. Recent advances in sample preparation and proteomic quantification platforms, specifically focused on recovery and enrichment of integral membrane proteins, are discussed.
Grant
Kathleen J
KJ
Department of Pharmacology, University of Colorado School of Medicine, Fitzsimons RC1 South, Aurora, CO 80045, USA.
Wu
Christine C
CC
D000818:Animals; D001923:Brain Chemistry; D002462:Cell Membrane / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis*; D008954:Models, Biological; D009474:Neurons / Q000648:ultrastructure*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods / Q000639:trends*; D013048:Specimen Handling; D013194:Staining and Labeling
D008565:Membrane Proteins; D020543:Proteome