Inhibitory effects of natural plants of Jeju Island on elastase and MMP-1 expression.
In order to search for new active cosmetic ingredients of natural origin, we screened about 60 plants collected from Jeju Island, which is located in the southernmost part of the Republic of Korea. We investigated their free radical scavenging activity, elastase inhibition activity, and reduction of MMP-1 mRNA expression for the development of anti-aging ingredients as raw materials for use in cosmetics. In the free radical scavenging capacity assay, 12 extracts, including Typha orientalis (seed) and Torreya nucifera (leaf), showed significant free radical scavenging activity (up to SC(50)<30 microg/ml). Among these extracts, Nymphaea tetragona (rhizome) extract showed the highest free radical scavenging activity (SC(50)=4.7 microg/ml). In the anti-elastase inhibition assay, seven extracts, including Typha orientalis (seed) and Persicaria hydropiper (whole plant), showed high inhibitory activity (>50% at 100 mug/ml). Among these extracts, Persicaria hydropiper (whole plant) extract showed the highest elastase inhibition activity (IC(50) = 46.7 mug/ml). In the MMP-1 expression assay using RT-PCR, Typha orientalis (seed), Pyrrosia hastata (root), and Capsicum annum (whole plant) showed slightly lower inhibition activity than EGCG, which was used as a control. Furthermore, four extracts, including Persicaria hydropiper (whole plant), Filipendula glaberrima (root), Nymphaea tetragona (root), and Camellia japonica (leaf), completely inhibited the expression of MMP-1 in human fibroblast cells. The results showed that four of the 60 plant extracts may hold potential for use as natural active ingredients for anti-aging cosmetics.
Kim
Young Heui
YH
R&D Center, Bioland Ltd., Byongchon, Chonan, Chungnam, 330-860, Republic of Korea.
Kim
Ki Soo
KS
Han
Chang Sung
CS
Yang
Hong Chul
HC
Park
Sun Hee
SH
Ko
Kang Ii
KI
Lee
Soo Hee
SH
Kim
Ki Ho
KH
Lee
Nam Ho
NH
Kim
Jung Mi
JM
Son
Kyung-Hun
KH
D001713:Biphenyl Compounds / Q000378:metabolism; D003358:Cosmetics / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D005562:Formazans / Q000737:chemistry; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D006834:Hydrazines / Q000378:metabolism; D007723:Korea; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D061965:Matrix Metalloproteinase Inhibitors; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D010851:Picrates; D010936:Plant Extracts / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D013778:Tetrazolium Salts / Q000737:chemistry
D001713:Biphenyl Compounds; D003358:Cosmetics; D004791:Enzyme Inhibitors; D005562:Formazans; D016166:Free Radical Scavengers; D006834:Hydrazines; D061965:Matrix Metalloproteinase Inhibitors; D010851:Picrates; D010936:Plant Extracts; D012333:RNA, Messenger; D013778:Tetrazolium Salts; C079782:MTT formazan; C004931:1,1-diphenyl-2-picrylhydrazyl; D010196:Pancreatic Elastase; D020781:Matrix Metalloproteinase 1