title: [The effect of cyclin D2 shRNA on the proliferation and apoptosis of LP-1 cell].
abstract: OBJECTIVE
  To construct cyclin D2 (CCND2) short hairpin RNA ( shRNA) plasmid for repressing the expression of CCND2 in human myeloma cell line LP-1,and to detect its effect on the proliferation and apoptosis of LP-1 cell.
  
  
  METHODS
  A CCND2 shRNA model was constructed and cloned into plasmid pGensil-2, then the plasmid was transfected into LP-1 cell in vitro. The CCND2 expression cell proliferation, cell cycle and cell apoptosis of the transfected LP-1 cells were studied by RT-PCR, trypanosome staining, flow cytometry and annexin V assay.
  
  
  RESULTS
  The transfection efficiency of LP-1 cell was 34. 2%. In the transfected LP-1 cell CCND2 mRNA expression was reduced significantly, the cell growth was inhibited significantly and the cell cycle was partly arrested in G, phase. The apoptosis rate of the transfected LP-1 cell after 72 h was (25.7+/-4.8)%.
  
  
  CONCLUSIONS
  The inhibition of CCND2 in LP-1 cells could inhibit the cell growth and induce cell apoptosis. CCND2 maybe a new therapeutic target.
authors.affiliation: Department of Hematology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
authors.forename: Cha
authors.initials: C
authors.lastname: Guo
authors.forename: Jian
authors.initials: J
authors.lastname: Hou
authors.forename: Yu-bao
authors.initials: YB
authors.lastname: Chen
authors.forename: Hui-yan
authors.initials: HY
authors.lastname: Jin
authors.forename: Wei-jun
authors.initials: WJ
authors.lastname: Fu
authors.forename: Zhen-gang
authors.initials: ZG
authors.lastname: Yuan
authors.forename: Dong-xing
authors.initials: DX
authors.lastname: Wang
mesh_terms: D017209:Apoptosis; D049109:Cell Proliferation; D056742:Cyclin D2; D016213:Cyclins / Q000235:genetics*; D006801:Humans; D034622:RNA Interference; D012330:RNA, Double-Stranded; D034741:RNA, Small Interfering; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: C080336:CCND2 protein, human; D056742:Cyclin D2; D016213:Cyclins; D012330:RNA, Double-Stranded; D034741:RNA, Small Interfering