The effect of growth medium on the antioxidant defense of Saccharomyces cerevisiae.
We compared the oxidation of dihydrorhodamine 123, glutathione contents and activities of superoxide dismutase (SOD) and catalase for three wild-type strains of Saccharomyces cerevisiae grown on media with different carbon sources. The rate of oxidation of dihydrorhodamine 123 was much higher in respiring cells grown on ethanol or glycerol media than in fermenting cells grown on glucose medium. The total SOD activity was highest on glycerol medium and lowest on ethanol medium, while the catalase activity was highest on glycerol medium. The sequence of glutathione content values was: glucose > ethanol > glycerol.
Macierzyńska
Ewa
E
Department of Molecular Biophysics, University of Łódź, Banacha 12/16, 90-237, Łódź, Poland. ematrix@biol.uni.lodz.pl
Grzelak
Agnieszka
A
Bartosz
Grzegorz
G
D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D003470:Culture Media / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D012235:Rhodamines / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D003470:Culture Media; D012235:Rhodamines; C058319:dihydrorhodamine 123; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione