title: Structural characterization of N-glycans of cauxin by MALDI-TOF mass spectrometry and nano LC-ESI-mass spectrometry.
abstract: Cauxin is a carboxylesterase-like glycoprotein excreted as a major component of cat urine. Cauxin contains four putative N-glycosylation sites. We characterized the structure of an N-linked oligosaccharide of cauxin using nano liquid chromatography (LC)-electrospray ionization (ESI) and matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry (MALDI-QIT-TOF MS) and MS/MS, and high-performance liquid chromatography (HPLC) with an octadecylsilica (ODS) column. The structure of the N-linked oligosaccharide of cauxin attached to (83)Asn was a bisecting complex type, Galbeta1-4GlcNAcbeta1-2Manalpha1-3(Galbeta1-4GlcNAcbeta1-2Manalpha1-6)(GlcNAcbeta1-4)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-6)GlcNAc.
authors.affiliation: Sphingolipid Expression Laboratory, Supra-Biomolecular System Research Group, RIKEN Frontier Research System, Wako, Saitama, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Masao
authors.initials: M
authors.lastname: Miyazaki
authors.forename: Emi
authors.initials: E
authors.lastname: Ito
authors.forename: Minoru
authors.initials: M
authors.lastname: Suzuki
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Yamashita
authors.forename: Hideharu
authors.initials: H
authors.lastname: Taira
authors.forename: Akemi
authors.initials: A
authors.lastname: Suzuki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D043182:Carboxylesterase / Q000737:chemistry*; D002415:Cats; D006031:Glycosylation; D008969:Molecular Sequence Data; D036103:Nanotechnology; D011134:Polysaccharides / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D011134:Polysaccharides; D043182:Carboxylesterase; C515680:cauxin protein, Felis catus