Variation of linker length in ratiometric fluorescent sensor proteins allows rational tuning of Zn(II) affinity in the picomolar to femtomolar range.
van Dongen
Elisabeth M W M
EM
Laboratory of Macromolecular and Organic Chemistry, Department of Biomedical Engineering, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, The Netherlands.
Evers
Toon H
TH
Dekkers
Linda M
LM
Meijer
E W
EW
Klomp
Leo W J
LW
Merkx
Maarten
M
D003432:Cross-Linking Reagents / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry*; D015032:Zinc / Q000737:chemistry*
D003432:Cross-Linking Reagents; D010455:Peptides; D011506:Proteins; D015032:Zinc