Susceptibility of mouse macrophage J774 to dengue virus infection.
The aim of this study was to investigate whether the J774 mouse macrophage cell line could be used as an in vitro model for dengue virus infection (DENV). After 3 days, infection in J774 cells was assessed by detecting dengue virus non-structural protein 1 (NSP-1) production either by dot blot or indirect immunofluorescence assay (IFA) of saponine-permeabilized J774 cells and then confirmed by RT-PCR (171 bp product, corresponding to the DENV-2 core). Based on the presence of NSP-1 in infected but not in non-infected cells by both IFA and dot blot, as well as the amplification of a 171-bp DENV-2-specific RT-PCR product exclusively in the infected cells, the J774 cell line was found to be permissive for dengue virus infection. As far as we know, this is the first report that the J774 mouse macrophage cell line is infected with dengue virus and, thus, that it can be used as an alternative in vitro model for dengue virus infection studies. This finding could help to further elucidate the mechanisms involved in dengue virus infection and pathogenesis.
Moreno-Altamirano
María M B
MM
Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México, D.F., México. mmoreno@encb.ipn.mx
Sánchez-García
F Javier
FJ
Legorreta-Herrera
Martha
M
Aguilar-Carmona
Israel
I
D000818:Animals; D002460:Cell Line; D003716:Dengue Virus / Q000235:genetics / Q000254:growth & development*; D019084:Fluorescent Antibody Technique, Indirect; D015151:Immunoblotting; D008264:Macrophages / Q000821:virology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012367:RNA, Viral / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017361:Viral Nonstructural Proteins / Q000235:genetics
C079340:NS1 protein, Dengue virus type 2; D012333:RNA, Messenger; D012367:RNA, Viral; D017361:Viral Nonstructural Proteins