title: p120-catenin is required for the collective invasion of squamous cell carcinoma cells via a phosphorylation-independent mechanism.
abstract: Loss of E-cadherin-mediated cell-cell junctions has been correlated with cancer cell invasion and poor patient survival. p120-catenin has emerged as a key player in promoting E-cadherin stability and adherens junction integrity and has been proposed as a potential invasion suppressor by preventing release of cells from the constraints imposed by cadherin-mediated cell-cell adhesion. However, it has been proposed that tyrosine phosphorylation of p120 may contribute to cadherin-dependent junction disassembly during invasion. Here, we use small interfering RNA (siRNA) in A431 cells to show that knockdown of p120 promotes two-dimensional migration of cells. In contrast, p120 knockdown impairs epidermal growth factor-induced A431 invasion into three-dimensional matrix gels or in organotypic culture, whereas re-expression of siRNA-resistant p120, or a p120 isoform that cannot be phosphorylated on tyrosine, restores the collective mode of invasion employed by A431 cells in vitro. Thus, p120 promotes A431 cell invasion in a phosphorylation-independent manner. We show that the collective invasion of A431 cells depends on the presence of cadherin-mediated (P- and E-cadherin) cell-cell contacts, which are lost in cells where p120 expression is knocked down. Furthermore, membranous p120 is maintained in invasive squamous cell carcinomas in tumours suggesting that p120 may be important for the collective invasion of tumours cells in vivo.
authors.affiliation: The Beatson Institute for Cancer Research, Glasgow, UK.
authors.forename: I R
authors.initials: IR
authors.lastname: Macpherson
authors.forename: S
authors.initials: S
authors.lastname: Hooper
authors.forename: A
authors.initials: A
authors.lastname: Serrels
authors.forename: L
authors.initials: L
authors.lastname: McGarry
authors.forename: B W
authors.initials: BW
authors.lastname: Ozanne
authors.forename: K
authors.initials: K
authors.lastname: Harrington
authors.forename: M C
authors.initials: MC
authors.lastname: Frame
authors.forename: E
authors.initials: E
authors.lastname: Sahai
authors.forename: V G
authors.initials: VG
authors.lastname: Brunton
mesh_terms: D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D051177:Catenins; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D045744:Cell Line, Tumor; D017931:DNA Primers; D004815:Epidermal Growth Factor / Q000378:metabolism; D006801:Humans; D009361:Neoplasm Invasiveness; D010750:Phosphoproteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D010766:Phosphorylation; D034741:RNA, Small Interfering; D011994:Recombinant Proteins / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism; D000095002:Delta Catenin
chemical_list: D051177:Catenins; D015815:Cell Adhesion Molecules; D017931:DNA Primers; D010750:Phosphoproteins; D034741:RNA, Small Interfering; D011994:Recombinant Proteins; D014443:Tyrosine; D004815:Epidermal Growth Factor; D000095002:Delta Catenin; C000722347:CTNND1 protein, human