Counter-current chromatography separation scaled up from an analytical column to a production column.
An analytical separation was performed on an analytical J-type counter-current chromatography (CCC) instrument using a 5.4 ml column, with a 1 ml/min mobile phase flow rate. This separation had a resolution of 0.69 and was achieved in 10 min. The same separation was performed using two 2300 ml columns connected in series at a flow rate of 850 ml/min using a production scale J-type centrifuge. This production scale separation was also obtained in 10 min with a resolution of 0.71. This represents an 850 times increase in productivity. This paper presents these separations and the underlying scale up theory.
Wood
Philip
P
Brunel Institute for Bioengineering, Brunel University, Uxbridge UB8 3PH, UK. philip.wood@brunel.ac.uk
Ignatova
Svetlana
S
Janaway
Lee
L
Keay
David
D
Hawes
David
D
Garrard
Ian
I
Sutherland
Ian A
IA
D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000295:instrumentation* / Q000379:methods* / Q000639:trends; D015203:Reproducibility of Results