Clinical significance of high-Km 5'-nucleotidase (cN-II) mRNA expression in high-risk myelodysplastic syndrome.
We analyzed cytosolic high-Km 5'-nucleotidase (cN-II) and deoxycytidine kinase (dCK) mRNA expression in bone marrow mononuclear cells (BMMNC) of patients with high-risk myelodysplastic syndrome (MDS) using quantitative real-time polymerase chain reaction (rt-PCR). At diagnosis, the cN-II mRNA expression of patients was higher than that of healthy volunteers, but the dCK mRNA expression showed no significant difference. Patients with ara-C-containing chemotherapies whose BMMNC showed a high level of cN-II expression (greater than the median value) had shorter median overall survival (15 months versus 22 months, p<0.01) and shorter median post-chemotherapy survival (10 months versus 16 months, p=0.012). These data suggest that the expression level of cN-II mRNA might be a prognostic factor of high-risk MDS.
Suzuki
Keijiro
K
Department of Hematology/Oncology, Iwate Medical University, Japan. ksuzuki-th@umin.ac.jp
Sugawara
Takeshi
T
Oyake
Tatsuo
T
Uchiyama
Toshiyuki
T
Aoki
Yusei
Y
Tsukushi
Yasuhiko
Y
Onodera
Shima
S
Ito
Shigeki
S
Murai
Kazunori
K
Ishida
Yoji
Y
D015720:5'-Nucleotidase / Q000096:biosynthesis* / Q000235:genetics; D000964:Antimetabolites, Antineoplastic / Q000627:therapeutic use; D001854:Bone Marrow Cells / Q000378:metabolism; D003561:Cytarabine / Q000627:therapeutic use; D003842:Deoxycytidine Kinase / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D053208:Kaplan-Meier Estimate; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D009190:Myelodysplastic Syndromes / Q000188:drug therapy / Q000201:enzymology* / Q000235:genetics; D011230:Precancerous Conditions / Q000201:enzymology / Q000235:genetics; D011379:Prognosis; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000964:Antimetabolites, Antineoplastic; D012333:RNA, Messenger; D003561:Cytarabine; D003842:Deoxycytidine Kinase; D015720:5'-Nucleotidase; C508048:NT5C2 protein, human