title: On mobility analysis of functional sites from time lapse microscopic image sequences of living cell nucleus.
abstract: Recent research in biology has indicated correlations between the movement patterns of functional sites (such as replication sites in DNA) and zones of genetic activity within a nucleus. A detailed study and analysis of the motion dynamics of these sites can reveal an interesting insight into their role in DNA replication and function. In this paper, we propose a suite of novel techniques to determine, analyze, and interpret the mobility patterns of functional sites. Our algorithms are based on interesting ideas from theoretical computer science and database theory and provide for the first time the tools to interpret the seemingly stochastic motion patterns of the functional sites within the nucleus in terms of a set of tractable 'patterns' which can then be analyzed to understand their biological significance.
authors.affiliation: Department of Computer Science and Engineering, State University of New York at Buffalo, USA. lm37@cse.buffalo.edu
authors.forename: Lopamudra
authors.initials: L
authors.lastname: Mukherjee
authors.forename: Vikas
authors.initials: V
authors.lastname: Singh
authors.forename: Jinhui
authors.initials: J
authors.lastname: Xu
authors.forename: Kishore S
authors.initials: KS
authors.lastname: Malyavantham
authors.forename: Ronald
authors.initials: R
authors.lastname: Berezney
mesh_terms: D000465:Algorithms; D001185:Artificial Intelligence; D002467:Cell Nucleus / Q000648:ultrastructure*; D004247:DNA / Q000648:ultrastructure*; D004261:DNA Replication; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D018715:Microscopy, Video / Q000379:methods*; D009038:Motion; D010363:Pattern Recognition, Automated / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013382:Subtraction Technique
chemical_list: D004247:DNA