[Lacrimal proteins electrophoretic analysis--diagnostic method in secondary Sjogren syndrome].
OBJECTIVE
We analysed and compared electrophoretic tear protein patterns of healthy subjects and patients with different autoimmune diseases associated with secondary Sjogren's syndrome.


METHODS
Tears were collected using the Schirmer's method. Proteins were separated by sodium-dodecyl sulfate poliacrylamide gel electrophoresis. The lanes were stained by Coomassie blue and/or silver.


RESULTS
Lactoferrin, albumin, lipocalin and lysozyme were found to be the main components being identified using molecular weight markers.


CONCLUSIONS
Electrophoretic analysis of tear proteins patterns is a fast, reproducible and simple method which provides information about the possibility of lacrimal gland involvement in autoimmmune diseases.
Cojocaru
Viorica Mădălina
VM
Spitalul Clinic de Urgenţe Oftalmologice, Bucureşti.
Ciurtin
Coziana
C
Puchianu
Elena
E
Antohe
Felicia
F
D000418:Albumins / Q000032:analysis; D000890:Anti-Infective Agents / Q000032:analysis; D001327:Autoimmune Diseases / Q000175:diagnosis; D002352:Carrier Proteins / Q000032:analysis; D016022:Case-Control Studies; D004591:Electrophoresis, Polyacrylamide Gel; D005136:Eye Proteins / Q000032:analysis*; D006801:Humans; D007781:Lactoferrin / Q000032:analysis; D054835:Lipocalin 1; D009113:Muramidase / Q000032:analysis; D012680:Sensitivity and Specificity; D012859:Sjogren's Syndrome / Q000175:diagnosis* / Q000276:immunology; D013666:Tears / Q000737:chemistry*
D000418:Albumins; D000890:Anti-Infective Agents; D002352:Carrier Proteins; D005136:Eye Proteins; C518443:LCN1 protein, human; D054835:Lipocalin 1; C042434:tear proteins; D009113:Muramidase; D007781:Lactoferrin