title: Thiols in nitric oxide synthase-containing Nocardia sp. strain NRRL 5646.
abstract: Mycothiol (MSH) [1-D-myo-inosityl-2-(N-acetyl-l-cysteinyl)amido-2-deoxy-alpha-D-glucopyranoside], isolated as the bimane derivative, was established to be the major thiol in Nocardia sp. strain NRRL 5646, a species most closely related to Nocardia brasiliensis strain DSM 43758(T). Thiol formation and detection of MSH-dependent formaldehyde dehydrogenase activity in cell extracts are relevant to the possible modulation of nitric oxide toxicity generated by strain NRRL 5646.
authors.affiliation: Division of Medicinal and Natural Products Chemistry, College of Pharmacy, and Center for Biocatalysis and Bioprocessing, The University of Iowa, Iowa City, IA 52242, USA.
authors.forename: Sungwon
authors.initials: S
authors.lastname: Lee
authors.forename: Hélène
authors.initials: H
authors.lastname: Bergeron
authors.forename: Peter C K
authors.initials: PC
authors.lastname: Lau
authors.forename: John P N
authors.initials: JP
authors.lastname: Rosazza
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000378:metabolism; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000032:analysis / Q000302:isolation & purification*; D006020:Glycopeptides / Q000032:analysis / Q000302:isolation & purification*; D007294:Inositol / Q000032:analysis / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009569:Nitric Oxide / Q000378:metabolism; D009615:Nocardia / Q000737:chemistry* / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D006020:Glycopeptides; D012336:RNA, Ribosomal, 16S; C089265:mycothiol; D009569:Nitric Oxide; D007294:Inositol; D000445:Aldehyde Oxidoreductases; C087006:formaldehyde dehydrogenase, glutathione-independent; D003545:Cysteine