High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy.
We report that single (or selective) plane illumination microscopy (SPIM), combined with a new deconvolution algorithm, provides a three-dimensional spatial resolution exceeding that of confocal fluorescence microscopy in large samples. We demonstrate this by imaging large living multicellular specimens obtained in a three-dimensional cell culture. The ability to rapidly image large samples at high resolution with minimal photodamage provides new opportunities especially for the study of subcellular processes in large living specimens.
Verveer
Peter J
PJ
Cell Biology & Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany. verveer@mpi-dortmund.mpg.de
Swoger
Jim
J
Pampaloni
Francesco
F
Greger
Klaus
K
Marcello
Marco
M
Stelzer
Ernst H K
EH
D000465:Algorithms; D000818:Animals; D002478:Cells, Cultured; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D012680:Sensitivity and Specificity