title: Expression of various CDC25B isoforms in human spermatozoa.
abstract: OBJECTIVE
  To explore the role of CDC25B protein in postmeiotic germ cells.
  
  
  METHODS
  In vitro experiment.
  
  
  METHODS
  University-based reproductive genetics laboratory.
  
  
  METHODS
  Fertile and infertile volunteers.
  
  
  METHODS
  Reverse transcription-polymerase chain reaction (RT-PCR), real-time RT-PCR, and immunostaining for CDC25B.
  
  
  METHODS
  Expression profiling of CDC25B in human spermatozoa.
  
  
  RESULTS
  Four splicing variants (CDC25B1, B2, B3, and B4) are expressed in human spermatozoa. Immunofluorescence staining showed strong homogeneous staining in the midpiece of spermatozoa, and weak staining in the principal piece and cytosol of the head. The messenger RNA (mRNA) transcript level of CDC25B was increased in sperm samples of men with asthenospermia.
  
  
  CONCLUSIONS
  The expression of CDC25B in different cellular compartments of human spermatozoa suggests that there are diverse noncell-cycle-related functions of CDC25B in terminally differentiated human germ cells.
authors.affiliation: Department of Biotechnology, Chia Nan University of Pharmacy and Science, National Cheng Kung University, College of Medicine, Tainan, Taiwan.
authors.forename: Yen-Ni
authors.initials: YN
authors.lastname: Teng
authors.forename: Chia-Ling
authors.initials: CL
authors.lastname: Chung
authors.forename: Yung-Ming
authors.initials: YM
authors.lastname: Lin
authors.forename: Hsien-An
authors.initials: HA
authors.lastname: Pan
authors.forename: Rui-Wen
authors.initials: RW
authors.lastname: Liao
authors.forename: Pao-Lin
authors.initials: PL
authors.lastname: Kuo
mesh_terms: D000328:Adult; D053627:Asthenozoospermia / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008297:Male; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013094:Spermatozoa / Q000378:metabolism*; D014018:Tissue Distribution; D020687:cdc25 Phosphatases / Q000235:genetics* / Q000378:metabolism / Q000502:physiology
chemical_list: D018797:Cell Cycle Proteins; D007527:Isoenzymes; C517008:CDC25B protein, human; D020687:cdc25 Phosphatases