title: Oligodendrocytes regulate formation of nodes of Ranvier via the recognition molecule OMgp.
abstract: The molecular mechanisms underlying the involvement of oligodendrocytes in formation of the nodes of Ranvier (NORs) remain poorly understood. Here we show that oligodendrocyte-myelin glycoprotein (OMgp) aggregates specifically at NORs. Nodal location of OMgp does not occur along demyelinated axons of either Shiverer or proteolipid protein (PLP) transgenic mice. Over-expression of OMgp in OLN-93 cells facilitates process outgrowth. In transgenic mice in which expression of OMgp is down-regulated, myelin thickness declines, and lateral oligodendrocyte loops at the node-paranode junction are less compacted and even join together with the opposite loops, which leads to shortened nodal gaps. Notably, each of these structural abnormalities plus modest down-regulation of expression of Na(+) channel alpha subunit result in reduced conduction velocity in the spinal cords of the mutant mice. Thus, OMgp that is derived from glia has distinct roles in regulating nodal formation and function during CNS myelination.
authors.affiliation: Institute of Molecular and Cell Biology, Singapore.
authors.forename: Du-Yu
authors.initials: DY
authors.lastname: Nie
authors.forename: Quan-Hong
authors.initials: QH
authors.lastname: Ma
authors.forename: Janice W S
authors.initials: JW
authors.lastname: Law
authors.forename: Chern-Pang
authors.initials: CP
authors.lastname: Chia
authors.forename: Narender K
authors.initials: NK
authors.lastname: Dhingra
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Shimoda
authors.forename: Wu-Lin
authors.initials: WL
authors.lastname: Yang
authors.forename: Neng
authors.initials: N
authors.lastname: Gong
authors.forename: Qing-Wen
authors.initials: QW
authors.lastname: Chen
authors.forename: Gang
authors.initials: G
authors.lastname: Xu
authors.forename: Qi-Dong
authors.initials: QD
authors.lastname: Hu
authors.forename: Pierce K H
authors.initials: PK
authors.lastname: Chow
authors.forename: Yee-Kong
authors.initials: YK
authors.lastname: Ng
authors.forename: Eng-Ang
authors.initials: EA
authors.lastname: Ling
authors.forename: Kazutada
authors.initials: K
authors.lastname: Watanabe
authors.forename: Tian-Le
authors.initials: TL
authors.lastname: Xu
authors.forename: Amyn A
authors.initials: AA
authors.lastname: Habib
authors.forename: Melitta
authors.initials: M
authors.lastname: Schachner
authors.forename: Zhi-Cheng
authors.initials: ZC
authors.lastname: Xiao