Immunodiagnosis of current fasciolosis in sheep naturally exposed to Fasciola hepatica by using a 2.9 kDa recombinant protein.
A 2.9 kDa recombinant-Fasciola hepatica protein (FhrAPS) was employed to estimate the prevalence of fasciolosis in sheep maintained under field conditions. For this purpose, 340 samples with known status in relation to fasciolosis by using a direct-ELISA and the coprological sedimentation were used. These samples were analysed by using an indirect-ELISA (iELISA) and the FhrAPS recombinant protein and excretory/secretory antigens (FhES) of this trematode. Current fasciolosis (CF) was named when results were positive to antigenemia and/or coprology. Out of 198 sheep with current fasciolosis, 68% were positive to the FhrAPS-ELISA test and 53% to the FhES. We observed 14% of the CF-neg sheep were positive to the FhrAPS, whereas this percentage was 52% with the FhES. A significant correlation between FhrAPS and current fasciolosis was obtained (r2=0.513, p=0.001). We concluded that the FhrAPS provides a more suitable antigen than FhES for developing field trials to know the prevalence of early and current fasciolosis.
Arias
M
M
Animal Pathology, Epidemiology, Parasitology and Parasitic Diseases and Zoonoses, College of Veterinary Medicine, University of Santiago de Compostela, 27001-Lugo, Spain.
Morrondo
P
P
Hillyer
G V
GV
Sánchez-Andrade
R
R
Suárez
J L
JL
Lomba
C
C
Pedreira
J
J
Díaz
P
P
Díez-Baños
P
P
Paz-Silva
A
A
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D005210:Fasciola hepatica; D005211:Fascioliasis / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D007074:Immunoglobulin G / Q000097:blood; D008969:Molecular Sequence Data; D011994:Recombinant Proteins; D012756:Sheep; D012757:Sheep Diseases / Q000097:blood / Q000175:diagnosis* / Q000469:parasitology
D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D011994:Recombinant Proteins