Characterization and function analysis of a cold-induced AmCIP gene encoding a dehydrin-like protein in Ammopiptanthus mongolicus.
A cDNA clone was isolated after difference screening from cotyledons of two-week cold-treated Ammopiptanthus mongolicus. The full-length cDNA sequence [designated as AmCIP (A. mongolicus cold-induced protein) gene] was 806 bp long and contained a 465 bp open reading frame (ORF) encoding a 16.6 kD protein of 154 amino acids. Bioinformatic analyses indicated that CIP belongs to dehydrin family with the features of high hydrophilicity, a helix K-segment, a long Gly-rich region and a phosphorylatable tract of Ser as well as deficiency in Cys and Trp. The expression of CIP gene increased after two weeks of cold treatment and more expression was detected in radicle than in cotyledon. And PCR amplification of the AmCIP gene from genome of A. mongolicus revealed this gene has no intron. Function prediction suggested this protein seems to protect the stabilization of membrane structure and prevent macromolecular coagulation or sequestrate calcium ions by association or disassociation with membrane under low temperature conditions.
Liu
Meiqin
M
College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, People's Republic of China.
Lu
Cunfu
C
Shen
Xin
X
Yin
Weilun
W
D000595:Amino Acid Sequence; D001483:Base Sequence; D003080:Cold Temperature; D018076:DNA, Complementary / Q000235:genetics; D007887:Fabaceae / Q000235:genetics* / Q000502:physiology; D006360:Heat-Shock Proteins / Q000737:chemistry* / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics* / Q000502:physiology
D018076:DNA, Complementary; D006360:Heat-Shock Proteins; D010940:Plant Proteins; C070556:dehydrin proteins, plant