title: [The effect of ligand of peroxisome proliferators-activated receptor gamma 15d-PGJ2 on the proliferation and activation of hepatic stellate cells].
abstract: OBJECTIVE
  To observe the effect of ligand of peroxisome proliferators-activated receptor gamma (PPAR gamma) 15d-PGJ2 on the proliferation and activation of hepatic stellate cells (HSC) and to study the role played by PPAR gamma during the process of HSC activation.
  
  
  METHODS
  By using RT-PCR and cell culture, we investigated the effects of 5 micro mol/L and 10 micro mol/L 15d-PGJ2 on culture-activated HSC and on PDGF-induced HSC proliferation, production of extracellular matrix and expression of chemokines.
  
  
  RESULTS
  The expression of alpha-SMA was significantly suppressed by 5mumol/L 15d-PGJ2, and the expression of PPAR gamma was significantly higher in the 15d-PGJ2 treated group than in the untreated group (0.64+/-0.03 vs 0.09+/-0.01, t=36.0517, P<0.01); PDGF-induced HSC proliferation was dose-dependently suppressed by 15d-PGJ2; the expressions of PPAR gamma in 5 micro mol/L and also in 10 micro mol/L 15d-PGJ2 plus PDGF pre-treated group increased much more than those in the PDGF-treated group (0.03+/-0.02 vs 0.60+/-0.03, t=42.6616, P<0.01 and 0.03+/-0.02 vs 0.69+/-0.04, t=33.83, P<0.01); the expressions of alpha-SMA, alpha 1 (I)-collagen and MCP-1 were suppressed.
  
  
  CONCLUSIONS
  Activation of PPAR gamma can modulate pro-fibrotic and pro-inflammatory roles of HSC and the increased expression of PPAR gamma may become a new target for antifibrosis.
authors.affiliation: Department of Gastroenterology, Tongji Hospital of Tongji University, Shanghai 200065, China.
authors.forename: Wen-zhuo
authors.initials: WZ
authors.lastname: Yang
authors.forename: Rui-lin
authors.initials: RL
authors.lastname: Liu
authors.forename: Min-de
authors.initials: MD
authors.lastname: Zeng
authors.forename: Lun-gen
authors.initials: LG
authors.lastname: Lu
authors.forename: Zhu-ping
authors.initials: ZP
authors.lastname: Fan
authors.forename: Shu-chang
authors.initials: SC
authors.lastname: Xu
authors.forename: Sheng-lan
authors.initials: SL
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Yang
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D055166:Hepatic Stellate Cells / Q000166:cytology* / Q000378:metabolism*; D008297:Male; D047495:PPAR gamma / Q000378:metabolism; D015230:Prostaglandin D2 / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
chemical_list: C477819:15-deoxyprostaglandin J2; D047495:PPAR gamma; D015230:Prostaglandin D2