title: [Identification of fetal nucleated erythrocytes in maternal blood using short tandem repeat typing after primer extension preamplification].
abstract: OBJECTIVE
  To develop a method for identifying fetal nucleated erythrocytes (NRBCs) in maternal blood.
  
  
  METHODS
  NRBCs in maternal blood were detected by benzidine staining and collected by micromanipulation. After primer extension preamplification (PEP) of the entire genome from a single NRBC, short tandem repeat (STR) genotype was analysed after further amplification of this gene. Single NRBC was differentiated as fetal or maternal origin by comparison of STR genotype of NRBC with its corresponding parents.
  
  
  RESULTS
  NRBCs were found in all of 28 pregnant women in a range of 4 to 13 per 5 ml venous blood. About 43. 6% of NRBCs were determined to be fetal origin by STR typing.
  
  
  CONCLUSIONS
  This method provides effective identification of fetal NRBCs and allows non-invasive prenatal genetic diagnosis using single fetal NRBC.
authors.affiliation: Department of Pediatrics, The First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China.
authors.forename: Hong-ying
authors.initials: HY
authors.lastname: Wei
authors.forename: Gui-fang
authors.initials: GF
authors.lastname: Long
authors.forename: Wei-xiong
authors.initials: WX
authors.lastname: Lin
authors.forename: Shu-quan
authors.initials: SQ
authors.lastname: Li
mesh_terms: D000328:Adult; D004900:Erythroblasts; D004906:Erythrocyte Count; D005260:Female; D005333:Fetus / Q000166:cytology; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D020411:Oligonucleotide Array Sequence Analysis; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D017086:beta-Thalassemia / Q000175:diagnosis*