[Study of susceptibility of oral bacteria biofilm to traditional Chinese drug preventing caries].
OBJECTIVE
Using MBEC-Assay to assay minimal biofilm eradication concentration (MBEC) of Galla Chinensis and Nidus Vespae to oral bacterial biofilm. To set up traditional Chinese medicine susceptibility pharmacodynamic empirical study methods of oral bacterial biofilm.


METHODS
Cariogenic bacteria strains were selected (Streptococcus mutans ATCC 25175, Streptococcus sanguis ATCC 10556, Lactobacillus rhamnosus AC 413, Actinomyces naeslundii WVU 627) in this study. Extraction components of Galla Chinensis were GCE (aqueous extract), GCE-B (30% alcohol extract) and extraction components of Nidus Vespae were NVE1 (95% alcohol extract). (1) To observe oral bacterial biofilm formatiom in MBEC-Device at different time. (2) MBEC-HTP-Assay: The minimal inhibitory concentration (MIC) and minimal biofilm eradication concentration (MBEC) of GCE, GCE-B and NVE1 to oral bacteria strains were determined.


RESULTS
Oral bacterial biofilm were readily formed on the lid of MBEC-Device under selected condition (observed by SEM). Oral cariogenic bacteria growing as plankton populations were sensitive to GCE, GCE-B and NVE1. To GCE, GCE-B and NVE1, oral cariogenic bacterial biofilm were 2-16 times less susceptible than growing plankton bacteria. GCE and GCE-B were the most effective medicine against oral cariogenic bacterial biofilm. NVE1 were effective in killing oral-bacterial biofilm at relatively high concentration.


CONCLUSIONS
GCE and GCE-B were effective medicine against oral cariogenic bacterial biofilm. MBEC (minimal biofilm eradication concentration) can provide a relative accurate medicine concentration for clinical test.
Zhao
Jin
J
Key Laboratory of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China.
Li
Ji-Yao
JY
Zhu
Bing
B
Zhou
Xue-Dong
XD
Xiao
Xiao-Rong
XR
D001419:Bacteria; D018441:Biofilms; D003731:Dental Caries; D008826:Microbial Sensitivity Tests; D013295:Streptococcus mutans; D013298:Streptococcus sanguis