title: Distribution of cren- and euryarchaeota in scots pine mycorrhizospheres and boreal forest humus.
abstract: Archaeal 16S rRNA gene sequences have been found in a variety of moderate-temperature habitats including soil and rhizospheres. In this study, the differences of archaeal communities associated with Scots pine (Pinus sylvestris L.) short roots, different types of mycorrhizospheric compartments, and uncolonized boreal forest humus were tested by direct DNA extraction, polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), and sequencing. The results indicated that mycorrhizal colonization of Scots pine roots substantially influence the archaeal community of pine rhizospheres. Colonization of short roots by most mycorrhizal fungi tested increased both archaeal frequency and diversity. Most of the archaeal sequences encountered in mycorrhizas belonged to the phylum Euryarchaeota, order of Halobacteriales. The difference in archaeal diversity between the mycorrhizospheric compartments and humus was profound. Most compartments with fungal components contained euryarchaeotal 16S rRNA gene sequences, whereas a high diversity of crenarchaeotal sequences and no euryarchaeotal sequences were found in forest humus outside mycorrhizospheres.
authors.affiliation: Department of Applied Biology, University of Helsinki, P.O. Box 27, Latokartanonkaari 7, FIN-00014, Helsinki, Finland.
authors.forename: Malin
authors.initials: M
authors.lastname: Bomberg
authors.forename: Sari
authors.initials: S
authors.lastname: Timonen
mesh_terms: D044822:Biodiversity; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D019605:Euryarchaeota / Q000145:classification / Q000235:genetics / Q000254:growth & development*; D008969:Molecular Sequence Data; D038821:Mycorrhizae / Q000254:growth & development*; D010802:Phylogeny; D041605:Pinus sylvestris / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D019641:DNA, Archaeal; D012336:RNA, Ribosomal, 16S