title: Tissue microarray-determined expression profiles of cyclooxygenase-2 in colorectal adenocarcinoma: association with clinicopathological parameters.
abstract: Accumulated evidence reveals that increased cyclooxygenase-2 (COX-2) is involved in the development of colorectal cancer. Our purpose was to quantitate COX-2 expression in colorectal cancers using tissue microarray analysis and look for an association with clinicopathological stage. Immunohistochemical analysis of COX-2 was performed in tissue microarray slides containing 90 specimens including 32 well-differentiated, 35 moderately differentiated, and 23 poorly differentiated colorectal adenocarcinomas. All colorectal adenocarcinomas showed significant immunohistochemical expression of COX-2 when compared to normal colon epithelia. However, there was no significant difference in immunostaining scores between poorly, moderately, and well-differentiated tumors (195 +/- 28, 214 +/- 26 and 200 +/- 24, respectively). The COX-2 immunostaining score correlated significantly with T stage (P < 0.05) but not with N or M stage. The positive expression rates of CK20 were 97% for well-differentiated, 94% for moderately differentiated, and 65% for poorly differentiated colorectal adenocarcinomas, suggesting that CK20 may not be an effective discriminator between poorly differentiated colorectal adenocarcinoma and metastatic adenocarcinoma.
authors.affiliation: Department of Pathology, Tri-Service General Hospital, Taipei , Taiwan 114, ROC.
authors.forename: Chi-Ying
authors.initials: CY
authors.lastname: Wu
authors.forename: Wei-Hwa
authors.initials: WH
authors.lastname: Lee
authors.forename: Jia-Yi
authors.initials: JY
authors.lastname: Wang
authors.forename: Hung
authors.initials: H
authors.lastname: Chiang
authors.forename: Junn-Liang
authors.initials: JL
authors.lastname: Chang
authors.forename: Wen-Chiuan
authors.initials: WC
authors.lastname: Tsai
authors.forename: Lai-Fa
authors.initials: LF
authors.lastname: Sheu
authors.forename: Jong-Shiaw
authors.initials: JS
authors.lastname: Jin
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology* / Q000473:pathology; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000473:pathology; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D046888:Tissue Array Analysis
chemical_list: D051546:Cyclooxygenase 2