[CD4(+) CD25(+) Tr cells and transcription factor Foxp3 in the naturally tolerance of rat liver transplantation].
OBJECTIVE
To investigate the role of intrahepatic CD4(+)CD25(+) T regulatory cells and Foxp3 gene in the natural tolerance in rat liver transplantation.


METHODS
The orthotopic liver transplantation models of inbred rats (LEW and DA rats) were established with double-sleeve technique and the models were divided into two groups: tolerance group (TOL group, LEW-to-DA) and rejection group (REJ group, DA-to-LEW). The intrahepatic lymphocytes from each group were isolated by using density gradient centrifugation. CD4(+)CD25(+) T cells were isolated by magic cell sorting system (MACS) and identified by flow cytometry (FCM). CD4(+)CD25(+) Tr cells suppression on the proliferation of CD4(+)CD25(-) T effector cells were analyzed by cell proliferation assay in vitro. Western blot was used to detect Scurfin protein expression of CD4(+)CD25(+) Tr cells.


RESULTS
CD4(+)CD25(+) Tr cells developed significantly greater in the TOL group than in the REJ group. In vitro, the spleen cells from LEW rats can irritate the proliferation of CD4(+)CD25(+) T cells more obviously than the syngeneic spleen cells. CD4(+)CD25(+) T cells could suppress the proliferation of CD4(+)CD25(-) T cells, but the inhibition was reversed by exogenous IL-2 (200 U/ml).


CONCLUSIONS
The immune suppression function of CD4(+)CD25(+) Tr cell, mediated by Foxp3 gene, is one of the mechanisms in liver transplantation tolerance.
Zhang
Feng
F
The Department of Liver Surgery, Liver Transplantation Center, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Lv
Ling
L
Pu
Li-Yong
LY
Li
Xiang-Cheng
XC
Yao
Ai-Hua
AH
Zhang
Wei
W
Yu
Yue
Y
Wang
Xue-Hao
XH
D000818:Animals; D015153:Blotting, Western; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D002478:Cells, Cultured; D005434:Flow Cytometry; D051858:Forkhead Transcription Factors / Q000378:metabolism*; D006084:Graft Rejection / Q000276:immunology; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology; D016031:Liver Transplantation / Q000276:immunology*; D008297:Male; D023421:Models, Animal; D051381:Rats; D011917:Rats, Inbred Lew; D011919:Rats, Inbred Strains; D023001:Transplantation Tolerance / Q000276:immunology*
D051858:Forkhead Transcription Factors; D053645:Interleukin-2 Receptor alpha Subunit