title: Separation of enantiomers of deprenyl with various CDs in CE and the effect of enantiomer migration order on enantiomeric impurity determination of selegiline in active ingredients and tablets.
abstract: Opposite affinity pattern of enantiomers of the antiparkinsonian chiral drug deprenyl (DEP) was observed towards various neutral and charged derivatives of -CD. The effect of the enantiomer migration order on the LOD of enantiomeric impurity of R-DEP (selegiline) was studied for the standard substances and in the tablets from three different suppliers. The influence of injection mode on the LOD of a minor enantiomeric impurity was also studied and the CE method was compared with the pharmacopoeial HPLC method using a commercially available chiral column Chiralcel OD-H. The optimized CE method was more suitable for low-level enantiomeric impurity determination in selegiline compared to the pharmacopoeial HPLC method.
authors.affiliation: Departamento de Química Analítica, Facultad de Química, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Castro-Puyana
authors.forename: Ketevan
authors.initials: K
authors.lastname: Lomsadze
authors.forename: Antonio
authors.initials: A
authors.lastname: LCrego
authors.forename: María Luisa
authors.initials: ML
authors.lastname: Marina
authors.forename: Bezhan
authors.initials: B
authors.lastname: Chankvetadze
mesh_terms: D000978:Antiparkinson Agents / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary; D012642:Selegiline / Q000032:analysis* / Q000737:chemistry; D013237:Stereoisomerism; D013607:Tablets; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D000978:Antiparkinson Agents; D013607:Tablets; D047392:beta-Cyclodextrins; D012642:Selegiline