title: Positioning of the mouse Hox gene clusters in the nuclei of developing embryos and differentiating embryoid bodies.
abstract: Expression of Hox genes located on different chromosomes is precisely regulated and synchronized during development. In order to test the hypothesis that the Hox loci might cluster in nuclear space in order to share regulatory components, we performed 3D FISH on cryosections of developing mouse embryos and differentiating embryoid bodies. We did not observe any instances of co-localization of 4 different Hox alleles. Instances of 2 different alleles touching each other were found in 20-47% of nuclei depending on the tissue. The frequency of such "kissing" events was not significantly different in cells expressing a high proportion of the Hox clusters when compared to cells expressing none or only a few Hox genes. We found that the HoxB and HoxC clusters, which are located in gene-rich regions, were involved more frequently in gene kissing in embryonic nuclei. In the case of HoxB, this observation correlated with the positioning of the corresponding chromosome towards the interior of the nucleus. Our results indicate that co-regulation of the different Hox clusters is not associated with co-localization of the loci at a single regulatory compartment and that the chromosomal context may influence the extent to which they contact each other in the nucleus.
authors.affiliation: Department Biology II, Ludwig-Maximilians University Munich, Grosshadernerstr. 2, Planegg-Martinsried, 82152, Germany. christian.lanctot@lrz.uni-muenchen.de
authors.forename: Christian
authors.initials: C
authors.lastname: Lanctôt
authors.forename: Cornelius
authors.initials: C
authors.lastname: Kaspar
authors.forename: Thomas
authors.initials: T
authors.lastname: Cremer
mesh_terms: D000483:Alleles; D000818:Animals; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D033481:Chromosomes, Mammalian / Q000235:genetics; D004622:Embryo, Mammalian / Q000378:metabolism* / Q000502:physiology; D053595:Embryonic Stem Cells / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D005801:Genes, Homeobox; D005865:Gestational Age; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D017404:In Situ Hybridization, Fluorescence; D008648:Mesoderm / Q000378:metabolism; D051379:Mice; D005810:Multigene Family
chemical_list: D018398:Homeodomain Proteins