Neuroprotective effect by Dammishimgyu-herbal acupuncture against H(2)O(2)-induced apoptosis in human neuroblastoma, SH-SY5Y cells.
BACKGROUND
The free radical is involved in neuronal cell death in human neurodegenerative disease. Dammishimgyu (DMSG)-herbal acupuncture has been used to treat neurological disorders in Korea. The present study was aimed to investigate the neuroprotective effect of DMSG-herbal acupuncture against H(2)O(2)-induced apoptosis in human neuroblastoma cell line, SH-SY5Y.


METHODS
The neuroprotective effect of DMSG-herbal acupuncture on H(2)O(2) induced apoptosis was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, 4,6-diamidino-2-phenylindole (DAPI) staining, reverse transcription-polymerase chain reaction (RT-PCR), western blots and nitrite assay.


RESULTS
In this study, 100 microM H(2)O(2)-treated cells decreased the cell viability with apoptotic features and increased the production of nitric oxide (NO). However, 0.1% DMSG treatment after exposure to 100 microM H(2)O(2) inhibited both H(2)O(2)-stimulated mRNA and protein expressions of BCL2-associated X protein (BAX) and caspase 3 apoptosis-related cysteine peptidase (CASP3). In addition, 0.1% DMSG treatment inhibited the increased NO production induced by H(2)O(2).


CONCLUSIONS
These results suggest that DMSG-herbal acupuncture shows protective effect against H(2)O(2)-induced neuronal damage.
Park
Hi-Joon
HJ
TKM Research Group, Kyung Hee University, Seoul 130-701, Korea.
Kim
Younjung
Y
Kim
Hakjae
H
Ha
Eunyoung
E
Park
Haejeong
H
Yoon
Seohyun
S
Kim
Mi-Ja
MJ
Choi
Sunmi
S
Ryu
Yeonhee
Y
Park
Hun-Kuk
HK
Hong
Meesuk
M
D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D004347:Drug Interactions; D005786:Gene Expression Regulation / Q000187:drug effects; D029001:Herbal Medicine; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007211:Indoles; D009447:Neuroblastoma; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009573:Nitrites / Q000378:metabolism; D013778:Tetrazolium Salts; D013844:Thiazoles; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
D007211:Indoles; D018696:Neuroprotective Agents; D009573:Nitrites; D013778:Tetrazolium Salts; D013844:Thiazoles; D051028:bcl-2-Associated X Protein; C007293:DAPI; D006861:Hydrogen Peroxide; D053148:Caspase 3; C022616:thiazolyl blue