Gastroprotective effect of swallow root (Decalepis hamiltonii) extract: possible involvement of H(+)-K(+)-ATPase inhibition and antioxidative mechanism.
The present study reports the antiulcer potentials of aqueous extract of swallow root (Decalepis hamiltonii Wight & Arn, SRAE) belonging to the family Asclepiadaceae. Swim stress-induced ulcers with an ulcer index (UI) of 6.0+/-0.01 was protected up to 43% and 72% at 100 and 200mg/kg b.w. of SRAE, respectively, similar to protection offered by ranitidine (79%) at 30mg/kg b.w. Depletion in antioxidant enzymes and increased Thiobarbituric Acid Reactive Substances (TBARS) were observed in ulcer-induced rats while SRAE fed rats showed normal levels. SRAE also normalized approximately 3.1 and 2.4 folds of increased H(+)-K(+)-ATPase and gastric mucin, respectively, in ulcerous animals, similar to the levels found in healthy controls. SRAE also possessed reducing power, free radical scavenging ability with an IC(50) of 0.17microg/mL gallic acid equivalent (GAE), comparable to that of BHA (IC(50)-0.08microg/mL). DNA protection up to 80% at 0.2microg was also observed. Toxicity studies indicated no lethal effects in rats fed up to 5g/kg b.w. Antioxidant, proton pump inhibition as well as boosting of gastric mucin effects of SRAE have been implicated to be responsible for antiulcer property of SRAE.
Naik
Yogender
Y
Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore 570020, Karnataka, India.
Jayaram
Smitha
S
Harish Nayaka
M A
MA
Lakshman
Dharmesh
Shylaja M
SM
D000818:Animals; D000897:Anti-Ulcer Agents / Q000627:therapeutic use*; D000975:Antioxidants / Q000627:therapeutic use*; D029062:Apocynaceae; D004305:Dose-Response Relationship, Drug; D005260:Female; D005752:Gastric Mucins / Q000032:analysis; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use; D054328:Proton Pump Inhibitors; D011899:Ranitidine / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D013276:Stomach Ulcer / Q000209:etiology / Q000517:prevention & control*; D013315:Stress, Psychological / Q000150:complications
D000897:Anti-Ulcer Agents; D000975:Antioxidants; D005752:Gastric Mucins; D010936:Plant Extracts; D054328:Proton Pump Inhibitors; D011899:Ranitidine