title: Neutrophil CD64 expression and serum IL-8: sensitive early markers of severity and outcome in sepsis.
abstract: The aim of the present study was to investigate which biomarker/s reliably assess severity and mortality early in the sepsis process. In 47 critically-ill patients within the 24h of septic onset, Interleukins (IL)-8, -1beta, -6, -10, and -12p70, tumor necrosis factor-alpha (TNF-alpha), procalcitonin (PCT) and C-reactive protein (CRP) were measured in serum. Additionally, CD64 expression was measured in neutrophils. In early sepsis, neutrophil CD64 expression and IL-8 levels are the only biomarkers that increased with sepsis severity, differentiating disease stages: sepsis, severe sepsis and septic shock (p<0.001). The biomarkers that best evaluate the severity of sepsis (via APACHE II) were CD64, IL-8 and IL-6 (p<0.01), and the severity of organ failure (via SOFA) were CD64 and IL-8 (p<0.01). CD64 expression and IL-8 levels were associated with mortality within 28-days (OR=1.3, p=0.01 for CD64 and OR=1.26, p=0.024 for IL-8 by logistic regression analysis) and ROC curve analysis showed high sensitivity and specificity for predicting sepsis stages and the 28 day mortality. We conclude that there is an early increase of neutrophil CD64 expression and IL-8 levels during sepsis. Based on this single measurement it is possible to reliably assess the stage, detect the severity and predict the 28-day mortality of sepsis.
authors: 3rd (Evgenidion Hospital), Department of Critical Care, University of Athens Medical School, 20 Papadiamantopoulou St., Athens, 115 28, Greece.
authors: Olga
authors: O
authors: Livaditi
authors: Anastasia
authors: A
authors: Kotanidou
authors: Aikaterini
authors: A
authors: Psarra
authors: Ioanna
authors: I
authors: Dimopoulou
authors: Christina
authors: C
authors: Sotiropoulou
authors: Kallirroi
authors: K
authors: Augustatou
authors: Chryssa
authors: C
authors: Papasteriades
authors: Apostolos
authors: A
authors: Armaganidis
authors: Charis
authors: C
authors: Roussos
authors: Stylianos E
authors: SE
authors: Orfanos
authors: Emmanuel E
authors: EE
authors: Douzinas
mesh_terms: D018806:APACHE; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000276:immunology*; D017452:Receptors, IgG / Q000097:blood*; D018805:Sepsis / Q000097:blood* / Q000175:diagnosis
chemical_list: D016209:Interleukin-8; D017452:Receptors, IgG