Characterization of A7r5 cell line transfected in a stable form by hSSAO/VAP-1 gene (A7r5 hSSAO/VAP-1 cell line).
A smooth muscle cell line (A7r5) was stably transfected with the human SSAO/VAP-1 (hSSAO/VAP-1) gene. The expressed protein was located solely in the membrane fraction of the cell. However it was also shown to be released into the cell-culture medium. Both the membrane-bound and released, soluble, forms had SSAO enzyme activity. Although MAO-A is present in wild-type A7r5 cells, it was undetectable in the transfected cells.
Solé
M
M
Departament de Bioquímica i Biologia Molecular, Facultat de Medicina, Institut de Neurociències, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
Hernandez
M
M
Boada
M
M
Unzeta
M
M
D006631:Amine Oxidase (Copper-Containing) / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000378:metabolism*; D002451:Cell Compartmentation / Q000235:genetics; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003470:Culture Media / Q000494:pharmacology; D006801:Humans; D008954:Models, Biological; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D051381:Rats; D014162:Transfection / Q000379:methods
D015815:Cell Adhesion Molecules; D003470:Culture Media; C076668:AOC3 protein, human; D006631:Amine Oxidase (Copper-Containing)