Structural biology of RNA silencing and its functional implications.
We outline structure-function contributions from our laboratories on protein-RNA recognition events that monitor siRNA length, 5 -phosphate and 2-nucleotide 3 overhangs, as well as the architecture of Argonaute, its externally bound siRNA complex, and Argonaute-based models involving guide-strand-mediated mRNA binding, cleavage, and release.
Patel
D J
DJ
Structural Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.
Ma
J-B
JB
Yuan
Y-R
YR
Ye
K
K
Pei
Y
Y
Kuryavyi
V
V
Malinina
L
L
Meister
G
G
Tuschl
T
T
D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis; D008954:Models, Biological; D008958:Models, Molecular; D010448:Peptide Initiation Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D034622:RNA Interference; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D043244:Ribonuclease III / Q000378:metabolism; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D035683:MicroRNAs; D010448:Peptide Initiation Factors; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D014764:Viral Proteins; D043244:Ribonuclease III