Evaluation of the antimicrobial mode of berberine by LC/ESI-MS combined with principal component analysis.
In this investigation, metabolic profiles of Staphylococcus aureus treated by berberine and nine antibacterial substances with known modes of action were acquired by HPLC/ESI-MS. After data pretreatment, those profiles acquired were reduced into several MS vectors. Then, principal component analysis was carried out upon those vectors to classify those drugs according to their mechanisms. From the result obtained by principal component analysis, the possible antibacterial mode of berberine was evaluated.
Yi
Zhi-Biao
ZB
College of Chemistry and Chemical Engineering, Research Center of Modernization of Chinese Medicines, Central South University, ChangSha 410083, PR China.
Yan Yu
Liang
Yi-Zeng
YZ
Bao Zeng
D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000145:classification / Q000494:pharmacology*; D001599:Berberine / Q000032:analysis / Q000494:pharmacology; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D005240:Feasibility Studies; D006358:Hot Temperature; D000432:Methanol / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D025341:Principal Component Analysis / Q000379:methods*; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics; D014867:Water / Q000737:chemistry
D000900:Anti-Bacterial Agents; D014867:Water; D001599:Berberine; D000432:Methanol