SWAP-70 is required for oncogenic transformation by v-Src in mouse embryo fibroblasts.
SWAP-70 is a phosphatidylinositol trisphosphate (PtdIns(3,4,5)P(3)) binding protein, which acts in F-actin rearrangement. The role of SWAP-70 in oncogenic transformation of mouse embryo fibroblasts (MEFs) by v-Src was examined by use of MEFs defective in SWAP-70. v-Src morphologically transformed MEFs lacking SWAP-70, but growth of the transformed cells in culture was slower than that of cells supplemented with exogenous SWAP-70. The v-Src-transformed MEFs deficient in SWAP-70 were unable to grow in soft agar while those expressing SWAP70 readily formed colonies, suggesting that SWAP-70 is required for anchorage independent growth of v-Src transformed MEFs. When transplanted in nude mice, tumors formed by the v-Src transformed SWAP-70(-/-) MEFs were smaller than those formed by cells expressing exogenous SWAP-70. These results suggest that SWAP-70 may be required for oncogenic transformation and contributes to cell growth in MEFs transformed by v-Src.
Fukui
Yasuhisa
Y
Division of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan. ayfukui@mail.ecc.u-tokyo.ac.jp
Tanaka
Takayuki
T
Tachikawa
Hiroyuki
H
Ihara
Sayoko
S
D000818:Animals; D002461:Cell Line, Transformed / Q000502:physiology*; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D004268:DNA-Binding Proteins / Q000502:physiology*; D004622:Embryo, Mammalian / Q000166:cytology*; D005347:Fibroblasts / Q000502:physiology*; D020662:Guanine Nucleotide Exchange Factors / Q000502:physiology*; D051379:Mice; D015778:Minor Histocompatibility Antigens; D009687:Nuclear Proteins / Q000502:physiology*; D015688:Oncogene Protein pp60(v-src) / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology
D004268:DNA-Binding Proteins; D020662:Guanine Nucleotide Exchange Factors; D015778:Minor Histocompatibility Antigens; D009687:Nuclear Proteins; C113365:Swap70 protein, mouse; D015688:Oncogene Protein pp60(v-src)