title: Karyotype with 210 chromosomes in guaraná (Paullinia cupana 'Sorbilis').
abstract: The genus Paullinia includes the economically important P. cupana, known as guaraná in Brazil and more recently in the world market. Native Americans of the Maué and Andirá tribes cultivated P. cupana 'Sorbilis' in central Amazon, and the Barés cultivated the 'Typica' variety in the upper Negro River (Brazil). Cytological studies in the Sapindaceae family have concentrated on the diversity in number (from 2n = 14 to 96) and size of the chromosomes. In Paullinia, seven species have been karyotyped and all show 2n = 24. Meristem maceration, cellular dissociation and air-drying techniques were used for cytogenetic preparations and DNA content was determined by flow cytometry. Chromosome characterization and DNA content of Paullinia cupana Kunth 'Sorbilis' (Mart.) Ducke (Sapindaceae) were studied. The high chromosome number (2n = 210) fall into two cytomorphological groups: (a) a metacentric and submetacentric group showing 25 sets of three pairs of chromosomes (2-76); (b) a group containing only acrocentric showing 12 sets of two pairs of chromosomes (82-105), a homologous submetacentric pair (1) and an acrocentric pair (81). Mean nuclear DNA content of guaraná was 2C = 22.8 pg. A karyogram was set up showing a high chromosome number complement.
authors: Instituto de Ciências Biológicas, Laboratório de Tecnologia de DNA, Universidade Federal do Amazonas, Manaus, AM, Brazil. danivalfreitas@hotmail.com
authors: Danival Vieira
authors: DV
authors: de Freitas
authors: Carlos Roberto
authors: CR
authors: Carvalho
authors: Firmino José do Nascimento
authors: FJ
authors: Filho
authors: Spartaco
authors: S
authors: Astolfi-Filho
mesh_terms: D001938:Brazil; D002110:Caffeine / Q000302:isolation & purification; D002874:Chromosome Mapping; D005434:Flow Cytometry; D017343:Genes, Plant; D007621:Karyotyping; D008677:Metaphase; D032126:Paullinia / Q000235:genetics*; D014329:Tropical Climate
chemical_list: D002110:Caffeine