The AAA-ATPase p97-Ufd1-Npl4 is required for ERAD but not for spindle disassembly in Xenopus egg extracts.
The highly abundant AAA-ATPase p97 is required for diverse cellular processes, of which ER-associated protein degradation (ERAD) is understood best. Previously, a new role of p97 in spindle disassembly at the end of mitosis has been reported. However, we show that neither addition of dominant-negative p97 mutants nor depletion of crucial p97 adaptors impairs transition of meiotic spindles into interphase arrays of microtubules. The dominant-negative approach is validated by inhibition of ERAD, which we reconstitute for the first time in the powerful biochemical system of Xenopus egg extracts. The role of p97 in spindle disassembly during meiotic exit should therefore be reconsidered.
Heubes
Simone
S
Department of Molecular Cell Biology, Max-Planck-Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.
Stemmann
Olaf
O
D000251:Adenosine Triphosphatases / Q000502:physiology*; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D006801:Humans; D009687:Nuclear Proteins / Q000502:physiology*; D010063:Ovum / Q000648:ultrastructure*; D008941:Spindle Apparatus; D014981:Xenopus; D029867:Xenopus Proteins / Q000502:physiology*
D017931:DNA Primers; D009687:Nuclear Proteins; C491498:UFD1 protein, Xenopus; D029867:Xenopus Proteins; D000251:Adenosine Triphosphatases; C404942:p97 ATPase