title: Oxidative stress and mitochondrial impairment can be separated from lipofuscin accumulation in aged human skeletal muscle.
abstract: According to the free radical theory of aging, reactive oxygen species (ROS) act as a driving force of the aging process, and it is generally believed that mitochondrial dysfunction is a major source of increased oxidative stress in tissues with high content of mitochondria, such as muscle or brain. However, recent experiments in mouse models of premature aging have questioned the role of mitochondrial ROS production in premature aging. To address the role of mitochondrial impairment and ROS production for aging in human muscles, we have analyzed mitochondrial properties in muscle fibres isolated from the vastus lateralis of young and elderly donors. Mitochondrial respiratory functions were addressed by high-resolution respirometry, and ROS production was analyzed by in situ staining with the redox-sensitive dye dihydroethidium. We found that aged human skeletal muscles contain fully functional mitochondria and that the level of ROS production is higher in young compared to aged muscle. Accordingly, we could not find any increase in oxidative modification of proteins in muscle from elderly donors. However, the accumulation of lipofuscin was identified as a robust marker of human muscle aging. The data support a model, where ROS-induced molecular damage is continuously removed, preventing the accumulation of dysfunctional mitochondria despite ongoing ROS production.
authors: Department for Molecular and Cellular Biology, Institute for Biomedical Aging Research of the Austrian Academy of Sciences, 6020 Innsbruck, Austria.
authors: Eveline
authors: E
authors: Hütter
authors: Mette
authors: M
authors: Skovbro
authors: Barbara
authors: B
authors: Lener
authors: Clara
authors: C
authors: Prats
authors: Rasmus
authors: R
authors: Rabøl
authors: Flemming
authors: F
authors: Dela
authors: Pidder
authors: P
authors: Jansen-Dürr
mesh_terms: D000328:Adult; D000368:Aged; D000375:Aging / Q000502:physiology*; D001706:Biopsy; D019069:Cell Respiration; D005260:Female; D006801:Humans; D008062:Lipofuscin / Q000378:metabolism*; D008297:Male; D008928:Mitochondria / Q000502:physiology*; D018482:Muscle, Skeletal / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology*; D010101:Oxygen Consumption; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D008062:Lipofuscin; D017382:Reactive Oxygen Species