[Preparation and identification of the oligonucleotide chip for detecting the virulence-associated genotypes and drug resistance of Helicobacter pylori].
OBJECTIVE
To develop an oligonucleotide microarray-based method for the simultaneous detection of Helicobacter pylori (Hp) infection, virulence-associated genotypes, and drug resistance.


METHODS
Hp was classified into cagA + and cagA- genotypes based on its virulence. Clarithromycin-resistance of Hp was identified by existence of point mutations in 23S rRNA. We constructed an oligonucleotide microarray chip to simultaneously diagnose Hp infection and detect its virulence-associated genotypes and mutations associated with clarithromycin-resistance. The diagnostic accuracy of the constructed microarray was tested with templates of wild type and mutated type.


RESULTS
The oligonucleotide chip accurately detected cagA + and cagA- genotypes of Hp, as well as four common point mutations in 23S rRNA related to clarithromycin-resistance.


CONCLUSIONS
Oligonucleotide microarray chip can be used to diagnose Hp infection and test its virulence-associated genotypes and drug resistance simultaneously.
Guo
Shu-Bin
SB
Department of Emergency, PUMC Hospital, CAMS and PUMC, Beijing 100730, China. shubinguo@medmail.com.cn
Liu
Jun-Bo
JB
Chen
Lin-Jie
LJ
Wang
Sheng-Qi
SQ
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D017291:Clarithromycin / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D005838:Genotype; D016480:Helicobacter pylori / Q000187:drug effects / Q000235:genetics* / Q000472:pathogenicity; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests; D020411:Oligonucleotide Array Sequence Analysis; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D014774:Virulence
D000900:Anti-Bacterial Agents; D017291:Clarithromycin