title: The zebrafish udu gene encodes a novel nuclear factor and is essential for primitive erythroid cell development.
abstract: Hematopoiesis is a complex process which gives rise to all blood lineages in the course of an organism's lifespan. However, the underlying molecular mechanism governing this process is not fully understood. Here we report the isolation and detailed study of a newly identified zebrafish ugly duckling (Udu) mutant allele, Udu(sq1). We show that loss-of-function mutation in the udu gene disrupts primitive erythroid cell proliferation and differentiation in a cell-autonomous manner, resulting in red blood cell (RBC) hypoplasia. Positional cloning reveals that the Udu gene encodes a novel factor that contains 2 paired amphipathic alpha-helix-like (PAH-L) repeats and a putative SANT-L (SW13, ADA2, N-Cor, and TFIIIB-like) domain. We further show that the Udu protein is predominantly localized in the nucleus and deletion of the putative SANT-L domain abolishes its function. Our study indicates that the Udu protein is very likely to function as a transcription modulator essential for the proliferation and differentiation of erythroid lineage.
authors: Laboratory of Molecular & Developmental Immunology, Institute of Molecular and Cell Biology, Proteos, Singapore.
authors: Yanmei
authors: Y
authors: Liu
authors: Linsen
authors: L
authors: Du
authors: Motomi
authors: M
authors: Osato
authors: Eng Hui
authors: EH
authors: Teo
authors: Feng
authors: F
authors: Qian
authors: Hao
authors: H
authors: Jin
authors: Fenghua
authors: F
authors: Zhen
authors: Jin
authors: J
authors: Xu
authors: Lin
authors: L
authors: Guo
authors: Honghui
authors: H
authors: Huang
authors: Jun
authors: J
authors: Chen
authors: Robert
authors: R
authors: Geisler
authors: Yun-Jin
authors: YJ
authors: Jiang
authors: Jinrong
authors: J
authors: Peng
authors: Zilong
authors: Z
authors: Wen
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D004913:Erythrocytes, Abnormal / Q000473:pathology; D041905:Erythroid Cells / Q000166:cytology; D015672:Erythroid Precursor Cells / Q000166:cytology*; D050981:Erythroid-Specific DNA-Binding Factors / Q000235:genetics / Q000502:physiology*; D004920:Erythropoiesis; D009154:Mutation; D014157:Transcription Factors / Q000502:physiology*; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000235:genetics / Q000502:physiology*
chemical_list: D050981:Erythroid-Specific DNA-Binding Factors; C522982:Gon4l protein, zebrafish; D014157:Transcription Factors; D029961:Zebrafish Proteins