Proteins of insoluble matrix of avian (gallus gallus) eggshell.
The protein composition of the insoluble avian eggshell matrix was studied. The determination of these proteins insoluble in water (EDTA-insoluble) was carried out using enzymatic cleavage followed by a high-performance liquid chromatography-mass spectrometry method. The influence of various enzymes on the protein splitting also was studied. The distribution of proteins depends on the type of layer (localization within the eggshell): ovocalyxin-32 was found mainly in the outer layer (the cuticle); ovocleidin-116 and 17 and ovocalyxin-36 were found throughout the whole eggshell, whereas ovalbumin was only found in the inner layer, the mammillary. The pigment (protoporphyrin IX) was mainly found in the cuticle and is incorporated into the protein network.
Miksík
Ivan
I
Institute of Physiology, Academy of Sciences of the Czech Republic, and Cardiovascular Research Centre, Prague, Czech Republic. miksik@biomed.cas.cz
Eckhardt
Adam
A
Sedláková
Pavla
P
Mikulikova
Katerina
K
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D004492:Edetic Acid; D004527:Egg Proteins / Q000032:analysis / Q000302:isolation & purification*; D004528:Egg Shell / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D011524:Protoporphyrins / Q000032:analysis; D012995:Solubility; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
D000596:Amino Acids; D004527:Egg Proteins; D011524:Protoporphyrins; C439069:ovocalyxin 32; C402224:ovocleidin-116; D004492:Edetic Acid; C028025:protoporphyrin IX