[Detection of Periplaneta americana sIgE with chemiluminescent immunoassay (CLIA)].
Crude extract of Periplaneta americana was prepared by liquid nitrogen grinding. After being purified with DEAE Sephadex A-50 ion exchange chromatography, the protein content of the extract was determined and the extract solution was prepared at gradient concentrations. The crude extract and purified allergen at different concentrations were dotted respectively on nitrocellulose (NC) membrane. Patient serum, bio-IgE, sa-HRP, luminal regents were added to the membrane. The chemiluminescence was displayed by exposing to X-film. The result revealed that the minimum protein content of crude Periplaneta americana extract detected by CLIA is 0.87 microg/ml, with 90% accordance to skin test positive patients, and 100% accordance to those with negative skin test and ELISA detection.
Wang
Qin
Q
Institute of Allergy and Immunology , Shenzhen University , Shenzhen 518060, China.
Liu
Zhi-Gang
ZG
Ji
Kun-Mei
KM
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006967:Hypersensitivity / Q000097:blood / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology*; D019476:Insect Proteins / Q000276:immunology; D008163:Luminescent Measurements / Q000379:methods*; D010526:Periplaneta / Q000276:immunology*; D015203:Reproducibility of Results
D019476:Insect Proteins; D007073:Immunoglobulin E