title: Study of animal species (human, dog and cat) identification using a multiplex single-base primer extension reaction in the cytochrome b gene.
abstract: We developed a simple method for animal species identification of humans, dogs and cats, using a multiplex single-base primer extension reaction in the cytochrome b gene. Using this method, three points of a single nucleotide in the cytochrome b gene were examined in these species using primers of different lengths. Our method was found to be able to successfully identify humans (26 samples), dogs (21 samples) and cats (9 samples), and no differences were found among the samples from each animal species in this study. The amount of template DNA required was over 0.01 ng for humans and dogs, and over 0.1 ng for cats. The present method was able to identify animal species from hair shaft (2 cm) and forensic casework samples (blood stains and hair shafts), and is thus a useful tool for animal species (human, dog and cat) identification in forensic science.
authors: Forensic Science Laboratory, Hiroshima Prefectural Police Headquarters, Kounan, Naka-ku 2-26-3, Hiroshima, Japan. fat35over@ybb.ne.jp
authors: Shin-ichi
authors: S
authors: Nakaki
authors: Daiki
authors: D
authors: Hino
authors: Miki
authors: M
authors: Miyoshi
authors: Hideki
authors: H
authors: Nakayama
authors: Hiroyuki
authors: H
authors: Moriyoshi
authors: Toshio
authors: T
authors: Morikawa
authors: Koji
authors: K
authors: Itohara
mesh_terms: D000818:Animals; D002415:Cats; D045303:Cytochromes b / Q000235:genetics*; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000302:isolation & purification*; D004285:Dogs; D006197:Hair / Q000737:chemistry; D006801:Humans; D009711:Nucleotides / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial; D009711:Nucleotides; D045303:Cytochromes b