title: Selective targeting of the retinal pigment epithelium in rabbit eyes with a scanning laser beam.
abstract: OBJECTIVE
  Selective targeting of the retinal pigment epithelium (RPE) with repetitive laser pulses that minimize thermal damage to the adjacent photoreceptors is a promising new therapeutic modality for RPE-related retinal diseases. The selectivity of an alternative, more versatile scanning approach was examined in vivo by using a broad range of scanning parameters.
  
  
  METHODS
  Acousto-optic deflectors repeatedly scanned the focus of a continuous wave (cw)-laser across the retina of Dutch belted rabbits, producing microsecond irradiation at each RPE cell. Two irradiation patterns forming separated lines (SEP) or interlaced lines (INT), different dwell times (2.5-75 micros), and repetition numbers (10 and 100 scans with 100-Hz repetition rate) were tested. Thresholds were evaluated by fundus imaging and angiography. Histology was performed for selected parameters.
  
  
  RESULTS
  Selective RPE cell damage was obtained with moderate laser power. The angiographic threshold power decreased with pulse duration, number of exposures, and applying the INT pattern. Ophthalmoscopic thresholds, indicating onset of thermal coagulation, were higher than twice the angiographic threshold for most tested parameters. Histology confirmed selective RPE cell damage for SEP irradiation with 7.5 and 15 micros; slower scan speeds or closed lines caused photoreceptor damage.
  
  
  CONCLUSIONS
  A cw-laser scanner can be set up as a highly compact and versatile device. Selective RPE damage is feasible with dwell times up to 15 micros. Greatest selectivity is achieved with short exposure times and separated scan lines. Interlaced lines and long exposure times facilitate heat conduction into photoreceptors. A scanner is an attractive alternative for pulsed selective targeting, because both selective targeting and thermal photocoagulation can be realized.
authors: Wellman Center for Photomedicine, Massachusetts General Hospital and Harvard Medical School, 185 Cambridge Street, Boston, MA 02114, USA.
authors: Carsten
authors: C
authors: Framme
authors: Clemens
authors: C
authors: Alt
authors: Susanne
authors: S
authors: Schnell
authors: Margaret
authors: M
authors: Sherwood
authors: Ralf
authors: R
authors: Brinkmann
authors: Charles P
authors: CP
authors: Lin
mesh_terms: D000818:Animals; D005131:Eye Injuries / Q000175:diagnosis; D005451:Fluorescein Angiography; D017075:Laser Coagulation / Q000009:adverse effects / Q000295:instrumentation / Q000379:methods*; D009887:Ophthalmoscopy; D020419:Photoreceptor Cells, Vertebrate / Q000473:pathology; D010857:Pigment Epithelium of Eye / Q000293:injuries / Q000473:pathology / Q000601:surgery*; D011817:Rabbits; D012160:Retina / Q000293:injuries / Q000473:pathology