title: Screening of beta-fructofuranosidase-producing microorganisms and effect of pH and temperature on enzymatic rate.
abstract: Seventeen different strains of filamentous fungi were grown in batch cultures to compare their abilities for the production of beta-fructofuranosidase. Three of them, Aspergillus oryzae IPT-301, Aspergillus niger ATCC 20611 and strain IPT-615, showed high production with total fructosyltransferase activity higher than 12,500 units l(-1). In addition, the beta-fructofuranosidases of those strains have a high fructosyltransferase activity-to-hydrolytic activity ratio. The temperature and pH effects on the sucrose-beta-fructofuranosidase reaction rate were studied using a 2(2) factorial experimental design. The comparative analysis of the tested variable coefficients shows that the variable pH contributes mostly to the changes in the fructosyltransferase and hydrolytic rates and in the V (t)/V (h) ratio. At 40 and 50 degrees C, there were no significant differences between the fructosyltransferase and hydrolytic velocities of these enzymes.
authors: Instituto Cubano de Investigaciones Azucareras-ICINAZ, Carretera al Central Manuel M Prieto Km 21/2, Boyeros, Havana, Cuba.
authors: Rubén Cuervo
authors: RC
authors: Fernandez
authors: Cristiane Angélica
authors: CA
authors: Ottoni
authors: Elda Sabino
authors: ES
authors: da Silva
authors: Rosa Mitiko Saito
authors: RM
authors: Matsubara
authors: José Márcio
authors: JM
authors: Carter
authors: Luis Roberto
authors: LR
authors: Magossi
authors: Maria Alice Alves
authors: MA
authors: Wada
authors: Maria Filomena
authors: MF
authors: de Andrade Rodrigues
authors: Beatriz Guilarte
authors: BG
authors: Maresma
authors: Alfredo Eduardo
authors: AE
authors: Maiorano
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D001236:Aspergillus oryzae / Q000201:enzymology; D005658:Fungi / Q000145:classification / Q000201:enzymology*; D006602:Hexosyltransferases / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology / Q000379:methods*; D009844:Oligosaccharides / Q000378:metabolism; D031786:Saccharum / Q000254:growth & development*; D012988:Soil Microbiology; D013696:Temperature; D043324:beta-Fructofuranosidase / Q000378:metabolism*; D001426:Bacterial Proteins
chemical_list: D006602:Hexosyltransferases; D009844:Oligosaccharides; D043324:beta-Fructofuranosidase; C116580:fructooligosaccharide; C021451:inulosucrase; D001426:Bacterial Proteins