Electrophoretic separation of environmentally important phenolic compounds using montomorillonite-coated fused-silica capillaries.
This paper reports a simple procedure for coating fused-silica capillaries with poly(diallyldimethyl ammonium chloride) and montmorillonite. The coated capillaries were characterized by performing EOF measurements as a function of buffer pH, number of layers of coating, and number of runs (stability). The coated capillaries showed a highly stable mu(EOF) (run-to-run RSD less than 1.5%, n = 20), allowing continuous use for several days without conditioning. The coated capillaries were then used for the effective separation of nine environmentally important phenolic compounds showing a significant improvement in the resolution, when compared to bare fused-silica capillaries. The EOF of the coated capillaries was constant in alkaline solutions (pH > or = 7), allowing the optimization of the separation conditions of phenolic compounds without significantly affecting the mu(EOF).
Mora
Maria F
MF
Department of Chemistry, The University of Texas at San Antonio, San Antonio, TX, USA.
García
Carlos D
CD
D001546:Bentonite; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D046209:Electrophoresis, Microchip / Q000379:methods; D004785:Environmental Pollutants / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D010636:Phenols / Q000302:isolation & purification*; D011095:Polyethylenes; D000644:Quaternary Ammonium Compounds; D015203:Reproducibility of Results; D012822:Silicon Dioxide
D004785:Environmental Pollutants; D010636:Phenols; D011095:Polyethylenes; D000644:Quaternary Ammonium Compounds; D001546:Bentonite; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride; D012822:Silicon Dioxide