Human single-chain Fv antibodies against Burkholderia mallei and Burkholderia pseudomallei.
Much effort has been devoted to the development of mouse monoclonal antibodies that react specifically with Burkholderia mallei and Burkholderia pseudomallei for diagnostic and/or therapeutic purposes. Our present study focused on the screening of a phage-displayed nonimmune human single-chain Fv (scFv) antibody library against heat-killed B. mallei and B. pseudomallei for the generation of human scFv antibodies specific to the two pathogenic species of bacteria. Using two different panning procedures, we obtained seven different scFv phage antibodies that interacted with the heat-killed whole bacterial cells of B. mallei and B. pseudomallei. Our results demonstrate that panning of a human scFv antibody library against heat-killed whole bacterial cells may provide a valuable strategy for developing human monoclonal antibodies against the highly pathogenic bacteria.
Zou
Nianxiang
N
Department of Environmental and Infectious Diseases, American Registry of Pathology, Armed Forces Institute of Pathology, Washington, DC 20306, USA.
Newsome
Tamara
T
Li
Bingjie
B
Tsai
Shien
S
Lo
Shyh-Ching
SC
D000818:Animals; D000907:Antibodies, Bacterial / Q000235:genetics / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology*; D042726:Burkholderia mallei / Q000276:immunology*; D016957:Burkholderia pseudomallei / Q000276:immunology*; D003001:Cloning, Molecular; D005896:Glanders / Q000175:diagnosis / Q000276:immunology; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics / Q000276:immunology*; D008554:Melioidosis / Q000175:diagnosis / Q000276:immunology; D051379:Mice
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D007135:Immunoglobulin Variable Region