title: Polyadenylation of a functional mRNA controls gene expression in Escherichia coli.
abstract: Although usually implicated in the stabilization of mRNAs in eukaryotes, polyadenylation was initially shown to destabilize RNA in bacteria. All the data are consistent with polyadenylation being part of a quality control process targeting folded RNA fragments and non-functional RNA molecules to degradation. We report here an example in Escherichia coli, where polyadenylation directly controls the level of expression of a gene by modulating the stability of a functional transcript. Inactivation of poly(A)polymerase I causes overexpression of glucosamine-6-phosphate synthase (GlmS) and both the accumulation and stabilization of the glmS transcript. Moreover, we show that the glmS mRNA results from the processing of the glmU-glmS cotranscript by RNase E. Interestingly, the glmU-glmS cotranscript and the mRNA fragment encoding GlmU only slightly accumulated in the absence of poly(A)polymerase, suggesting that the endonucleolytically generated glmS mRNA harbouring a 5' monophosphate and a 3' stable hairpin is highly susceptible to poly(A)-dependent degradation.
authors: Régulation de l'Expression Génétique chez les Microorganismes, UPR CNRS no 9073, conventionnée avec l'Université Paris 7-Denis Diderot, Paris, France.
authors: Géraldine
authors: G
authors: Joanny
authors: Jacques
authors: J
authors: Le Derout
authors: Dominique
authors: D
authors: Bréchemier-Baey
authors: Valérie
authors: V
authors: Labas
authors: Joelle
authors: J
authors: Vinh
authors: Philippe
authors: P
authors: Régnier
authors: Eliane
authors: E
authors: Hajnsdorf
mesh_terms: D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing) / Q000235:genetics* / Q000378:metabolism; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism*; D026723:Polyadenylation; D011062:Polynucleotide Adenylyltransferase / Q000235:genetics; D020871:RNA Stability; D012333:RNA, Messenger / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D029968:Escherichia coli Proteins; C520333:GlmU protein, E coli; D009097:Multienzyme Complexes; D012333:RNA, Messenger; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing); D011062:Polynucleotide Adenylyltransferase; C063643:pcnB protein, E coli