Transcription antitermination by translation initiation factor IF1.
Bacterial translation initiation factor IF1 is an S1 domain protein that belongs to the oligomer binding (OB) fold proteins. Cold shock domain (CSD)-containing proteins such as CspA (the major cold shock protein of Escherichia coli) and its homologues also belong to the OB fold protein family. The striking structural similarity between IF1 and CspA homologues suggests a functional overlap between these proteins. Certain members of the CspA family of cold shock proteins act as nucleic acid chaperones: they melt secondary structures in nucleic acids and act as transcription antiterminators. This activity may help the cell to acclimatize to low temperatures, since cold-induced stabilization of secondary structures in nascent RNA can impede transcription elongation. Here we show that the E. coli translation initiation factor, IF1, also has RNA chaperone activity and acts as a transcription antiterminator in vivo and in vitro. We further show that the RNA chaperone activity of IF1, although critical for transcription antitermination, is not essential for its role in supporting cell growth, which presumably functions in translation. The results thus indicate that IF1 may participate in transcription regulation and that cross talk and/or functional overlap may exist between the Csp family proteins, known to be involved in transcription regulation at cold shock, and S1 domain proteins, known to function in translation.
Phadtare
Sangita
S
Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA. phadtasa@umdnj.edu
Kazakov
Teymur
T
Bubunenko
Mikhail
M
Court
Donald L
DL
Pestova
Tatyana
T
Severinov
Konstantin
K
D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial; D005816:Genetic Complementation Test; D006639:Histidine / Q000235:genetics / Q000378:metabolism; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D039861:Prokaryotic Initiation Factor-1 / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D011485:Protein Binding; D014158:Transcription, Genetic / Q000235:genetics*
D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D018832:Molecular Chaperones; D039861:Prokaryotic Initiation Factor-1; D006639:Histidine