[A rapid method for preparation of plasmid DNA for screening recombinant clones].
A simple and rapid method for preparation of plasmid DNA from overnight incubation was introduced. It does not require any additional reagents; the incubation mixture containing recombinant plasmid DNA was just mixed with H2O and phenol/chloroform/isoamyl alcohol in certain ratio. After vortexing and spinning of the mixture, the supernatant could be directly loaded onto agarose gel and analyzed using electrophoresis. The whole preparation requires only 3-5 minutes. So to quickly screen recombinant clones, this method is better compared with traditional methods.
Guo
Xu-Dong
XD
The Key Laboratory for Mammalian Reproductive Biology and Biotechnology, Ministry of Education, Inner Mongolia University, Hohhot 010021, China.
Mao
Shu-Yan
SY
Hou
Dong-Xia
DX
Bou
Shorgan
S
D002499:Centrifugation, Density Gradient; D002725:Chloroform / Q000737:chemistry; D003001:Cloning, Molecular / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D015247:Deoxyribonuclease HindIII / Q000378:metabolism; D004586:Electrophoresis; D004926:Escherichia coli / Q000235:genetics*; D000439:Pentanols / Q000737:chemistry; D019800:Phenol / Q000737:chemistry; D010957:Plasmids / Q000737:chemistry / Q000235:genetics*; D015203:Reproducibility of Results; D013997:Time Factors; D014867:Water / Q000737:chemistry
D004269:DNA, Bacterial; D000439:Pentanols; D014867:Water; D019800:Phenol; D002725:Chloroform; C029683:isopentyl alcohol; D015247:Deoxyribonuclease HindIII