Cloning and expression of the gene encoding the diapause hormone and pheromone biosynthesis activating neuropeptide of the beet armyworm, Spodoptera exigua.
Diapause hormone (DH) and pheromone biosynthesis activating neuropeptide (PBAN), two important insect neuropeptides, regulate insect development and sex pheromone biosynthesis, respectively. DH-like immunoreactivity has been detected in the suboesophageal ganglion (SG) of pharate adult of Spodoptera exigua (Spe) by using an antiserum against Helicoverpa armigera DH. A full-length of Spe-DH-PBAN cDNA was obtained based on reverse transcription-PCR and rapid amplification of cDNA ends strategies. The open reading frame of this cDNA encodes a 197-amino acid precursor protein that contains DH, PBAN, and three other SG neuropeptides, all of which share a conservative C-terminal pentapeptide motif FXPR/KL (X = G, T or S). Northern blot analysis demonstrates the presence of an 800 bp transcript in the SG. The Spe-DH-PBAN mRNA is detectable at high levels at larval and adult stages, suggesting that Spe-DH-PBAN gene might be correlated with larval development and sex pheromone biosynthesis in moths.
Xu
Jun
J
Department of Molecular and Cell Biology, School of Life Sciences, University of Science and Technology of China, Hefei 230027, People's Republic of China.
Su
Jian-Ya
JY
Shen
Jin-Liang
JL
Xu
Wei-Hua
WH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D007150:Immunohistochemistry; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D018411:Spodoptera / Q000235:genetics*; D014018:Tissue Distribution
D018076:DNA, Complementary; D019476:Insect Proteins; D009479:Neuropeptides; C075027:diapause hormone