title: A rat surgical model of esophageal metaplasia and adenocarcinoma-induced by mixed reflux of gastric acid and duodenal contents.
abstract: Recent clinical data have revealed that mixed reflux (MR) of gastric acid and duodenal contents frequently occurs in patients with gastroesophageal reflux disease and a progressive increase of MR occurs with increasing severity across gastroesophageal reflux disease. Herein we report a novel rat surgical model in which esophageal metaplasia and adenocarcinoma develop as complications of MR. The model was created by performing an esophagojejunostomy and a gastrojejunostomy 5 mm proximal to the esophagojejunal anastomosis in 40 rats. Severe inflammatory and proliferative changes, high prevalence of esophageal metaplasia (78%), and adenocarcinoma (50%) were observed in the lower part of the esophagus of rats 20 weeks after surgery. The resulting esophageal lesions resembled those described in humans and supported a progression from intestinal metaplasia to dysplasia and, ultimately, esophageal adenocarcinoma. Such a model may provide a useful tool in study of human reflux-induced carcinogenesis.
authors: Department of Thoracic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710038, China.
authors: Tao
authors: T
authors: Zhang
authors: Feng
authors: F
authors: Zhang
authors: Yong
authors: Y
authors: Han
authors: Zhongping
authors: Z
authors: Gu
authors: Yong'an
authors: Y
authors: Zhou
authors: Qingshu
authors: Q
authors: Cheng
authors: Yifang
authors: Y
authors: Zhu
authors: Chuanshan
authors: C
authors: Zhang
authors: Yunjie
authors: Y
authors: Wang
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology / Q000601:surgery*; D000818:Animals; D004195:Disease Models, Animal; D018450:Disease Progression; D004385:Duodenostomy / Q000379:methods*; D004386:Duodenum / Q000378:metabolism; D004938:Esophageal Neoplasms / Q000209:etiology / Q000473:pathology / Q000601:surgery*; D004946:Esophagostomy / Q000379:methods*; D004947:Esophagus / Q000473:pathology* / Q000601:surgery; D005744:Gastric Acid / Q000378:metabolism; D005764:Gastroesophageal Reflux / Q000150:complications* / Q000378:metabolism; D007582:Jejunostomy / Q000379:methods*; D008297:Male; D008679:Metaplasia / Q000209:etiology / Q000473:pathology / Q000601:surgery; D008953:Models, Anatomic; D051381:Rats; D017207:Rats, Sprague-Dawley