title: Cloning and expression of the gene encoding the diapause hormone and pheromone biosynthesis activating neuropeptide of the beet armyworm, Spodoptera exigua.
abstract: Diapause hormone (DH) and pheromone biosynthesis activating neuropeptide (PBAN), two important insect neuropeptides, regulate insect development and sex pheromone biosynthesis, respectively. DH-like immunoreactivity has been detected in the suboesophageal ganglion (SG) of pharate adult of Spodoptera exigua (Spe) by using an antiserum against Helicoverpa armigera DH. A full-length of Spe-DH-PBAN cDNA was obtained based on reverse transcription-PCR and rapid amplification of cDNA ends strategies. The open reading frame of this cDNA encodes a 197-amino acid precursor protein that contains DH, PBAN, and three other SG neuropeptides, all of which share a conservative C-terminal pentapeptide motif FXPR/KL (X = G, T or S). Northern blot analysis demonstrates the presence of an 800 bp transcript in the SG. The Spe-DH-PBAN mRNA is detectable at high levels at larval and adult stages, suggesting that Spe-DH-PBAN gene might be correlated with larval development and sex pheromone biosynthesis in moths.
authors: Department of Molecular and Cell Biology, School of Life Sciences, University of Science and Technology of China, Hefei 230027, People's Republic of China.
authors: Jun
authors: J
authors: Xu
authors: Jian-Ya
authors: JY
authors: Su
authors: Jin-Liang
authors: JL
authors: Shen
authors: Wei-Hua
authors: WH
authors: Xu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D007150:Immunohistochemistry; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D018411:Spodoptera / Q000235:genetics*; D014018:Tissue Distribution
chemical_list: D018076:DNA, Complementary; D019476:Insect Proteins; D009479:Neuropeptides; C075027:diapause hormone