Microbial transformation of 5alpha,6alpha-epoxy-3beta-hydroxy-16-pregnen-20-one by Trichoderma viride.
Fermentation of 5alpha,6alpha-epoxy-3beta-hydroxy-16-pregnen-20-one (4) with Trichoderma viride under aerobic condition yielded 3beta,5alpha,6beta-trihydroxy-16-pregnen-20-one (5) and 3beta,5alpha,6beta,15beta-tetrahydroxy-16-pregnen-20-one (6). Each microbial metabolite was characterized by spectroscopic methods. Compounds 6 and 3beta,5alpha,15beta-trihydroxy-16-pregnen-6,20-dione (7) are reported for the first time.
Liu
Hong-Min
HM
Zhengzhou University, Department of Chemistry, No. 75 Da Xue North Road, ZhengZhou, Henan 450052, China. liuhm@zzu.edu.cn
Ge
Wenzhong
W
Li
Heping
H
Wu
Jian
J
D001711:Biotransformation; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D011280:Pregnanolone / Q000031:analogs & derivatives* / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D014242:Trichoderma / Q000378:metabolism*
C521989:5alpha,6alpha-epoxy-3beta-hydroxy-16-pregnen-20-one; D011280:Pregnanolone