Antioxidant activity of Vaccinium stamineum: exhibition of anticancer capability in human lung and leukemia cells.
Fruit of deerberry [Vaccinium stamineum L.] were evaluated for their antioxidant capacity and anticancer properties in JB6 P (+) mouse epidermal cells, human lung and leukemia cells. Deerberries contain potent free radical scavenging activities. Pretreatment of JB6 P (+) mouse epidermal cells with deerberry fruit extracts produced an inhibition on the activation of activator protein-1 (AP-1) and nuclear factor-kappaB (NF-kappaB) induced by either 12- O-tetradecanoylphorbol 13-acetate (TPA) or ultraviolet-B (UVB). Deerberry fruit extracts also blocked TPA- or UVB-induced phosphorylation of ERKs and MEK 1/2, two upstream regulators of AP-1 and inhibited proliferation of human leukemia HL-60 cancer cells and human lung epithelial cancer A549 cells and induced apoptosis of HL-60 cells. These results suggest that the inhibition of TPA- or UVB-induced AP-1 and NF-kappaB activity, inhibition of HL-60 cells and cancer A549 cells proliferation and induction of apoptotic in human leukemia HL-60 cancer cells may be mediated through the ERKs and MEK 1/2 signal pathway.
Wang
Shiow Y
SY
Fruit Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, U. S. Department of Agriculture, Beltsville, MD 20705-2350, USA. wangs@ba.ars.usda.gov
Feng
Rentian
R
Bowman
Linda
L
Lu
Yongju
Y
Ballington
James R
JR
Ding
Min
M
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004789:Enzyme Activation / Q000187:drug effects / Q000528:radiation effects; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification; D005638:Fruit / Q000737:chemistry; D006801:Humans; D007938:Leukemia / Q000201:enzymology / Q000378:metabolism; D008175:Lung Neoplasms / Q000201:enzymology / Q000378:metabolism; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D016328:NF-kappa B / Q000037:antagonists & inhibitors; D010766:Phosphorylation / Q000187:drug effects / Q000528:radiation effects; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D018808:Transcription Factor AP-1 / Q000037:antagonists & inhibitors; D014466:Ultraviolet Rays; D029796:Vaccinium / Q000737:chemistry*
D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D005419:Flavonoids; D016328:NF-kappa B; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D018808:Transcription Factor AP-1; C521777:Vaccinium stamineum fruit extract; D020928:Mitogen-Activated Protein Kinases; D013755:Tetradecanoylphorbol Acetate