title: The complete mitochondrial genome of the Korean soft-shelled turtle Pelodiscus sinensis (Testudines, Trionychidae).
abstract: We isolated Korean soft-shelled turtle, Pelodiscus sinensis, mitochondrial DNA by long-polymerase chain reaction (long-PCR) with conserved primers and sequenced this mitochondrial genome (mitogenome) with primer walking using flanking sequences. The P. sinensis mitochondrial DNA has 17,042 bp and its structural organization is conserved compared to those of other reptiles and mammals. To unveil the phylogenetic relationship of the turtles, we used the NJ, MP, and ML analysis methods after inferring those sequences from the mitochondrial 16S rRNA gene. We also compared two P. sinensis variants from Korea and China using the mitochondrial genome. In this study, we report the basic characteristics of the P. sinensis mitochondrial genome, including structural organization and base composition of the rRNAs, tRNAs and protein-coding genes, as well as characteristics of tRNAs. These features are applicable for the study of phylogenetic relationships in turtles.
authors: Department of Molecular and Environmental Bioscience, Graduate School, Hanyang University, Seoul 133-791, Korea
authors: Sang-Oun
authors: SO
authors: Jungt
authors: Young-Mi
authors: YM
authors: Lee
authors: Yuri
authors: Y
authors: Kartavtsev
authors: In-Seok
authors: IS
authors: Park
authors: Dong Soo
authors: DS
authors: Kim
authors: Jae-Seong
authors: JS
authors: Lee
mesh_terms: D000818:Animals; D001482:Base Composition; D020029:Base Pairing; D001483:Base Sequence; D002681:China; D016000:Cluster Analysis; D017124:Conserved Sequence / Q000235:genetics; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics*; D023061:Gene Order; D007723:Korea; D016013:Likelihood Functions; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014426:Turtles / Q000235:genetics*
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial