title: Phosphorylation at serine 208 of the 1alpha,25-dihydroxy Vitamin D3 receptor modulates the interaction with transcriptional coactivators.
abstract: Upon ligand binding the 1alpha,25-dihydroxy Vitamin D3 receptor (VDR) undergoes a conformational change that allows interaction with coactivator proteins including p160/SRC family members and the multimeric DRIP complex through the DRIP205 subunit. Casein kinase II (CKII) phosphorylates VDR both in vitro and in vivo at serine 208 within the hinge domain. This phosphorylation does not affect the ability of VDR to bind DNA, but increases its ability to transactivate target promoters. Here, we have analyzed whether phosphorylation of VDR by CKII modulates the ability of VDR to interact with coactivators in vitro. We find that both mutation of serine 208 to aspartic acid (VDRS208D) or phosphorylation of VDR by CKII enhance the interaction of VDR with DRIP205 in the presence of 1alpha,25-dihydroxy Vitamin D3. We also find that the mutation VDRS208D neither affects the ability of this protein to bind DNA nor to interact with SRC-1 and RXRalpha. Together, our results indicate that phosphorylation of VDR at serine 208 contributes to modulate the affinity of VDR for the DRIP complex and therefore may have a role in vivo regulating VDR-mediated transcriptional enhancement.
authors: Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias Biologicas, Universidad de Concepcion, Casilla 160-C, Concepcion, Chile.
authors: Gloria
authors: G
authors: Arriagada
authors: Roberto
authors: R
authors: Paredes
authors: Juan
authors: J
authors: Olate
authors: Andre
authors: A
authors: van Wijnen
authors: Jane B
authors: JB
authors: Lian
authors: Gary S
authors: GS
authors: Stein
authors: Janet L
authors: JL
authors: Stein
authors: Sergio
authors: S
authors: Onate
authors: Martin
authors: M
authors: Montecino
mesh_terms: D009154:Mutation / Q000235:genetics; D010768:Phosphoserine / Q000378:metabolism*; D011485:Protein Binding; D018167:Receptors, Calcitriol / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators / Q000378:metabolism*
chemical_list: D018167:Receptors, Calcitriol; D015534:Trans-Activators; D010768:Phosphoserine