Identification of phostensin, a PP1 F-actin cytoskeleton targeting subunit.
We have identified a novel protein, protein phosphatase 1 F-actin cytoskeleton targeting subunit (phostensin). This protein is encoded by KIAA1949 and was found to associate with protein phosphatase 1 (PP1) in the yeast two-hybrid assay, co-immunoprecipitation, and GST pull-down assay. Northern blot analysis revealed that phostensin mRNA was predominantly distributed in leukocytes and spleen, and phostensin protein was present in crude extracts of human peripheral leukocytes. Immunofluorescence microscopic analysis revealed that the phostensin/PP1 complex was conspicuously localized with the actin cytoskeleton at the cell periphery in Madin-Darby canine kidney (MDCK) epithelial cells. Taken together, our data shows that phostensin targets PP1 to F-actin cytoskeleton. The phostensin/PP1 complex may play a vital role in modulation of actin rearrangements.
Kao
Shu-Chen
SC
Department of Life Science, National Chung Hsing University, Taichung, Taiwan.
Chen
Chun-Yu
CY
Wang
Shiu-Lan
SL
Yang
Jaw-Ji
JJ
Hung
Wei-Chuan
WC
Chen
Yi-Chen
YC
Lai
Ning-Sheng
NS
Liu
Hsin-Tzu
HT
Huang
Hsien-lu
HL
Chen
Hong-Chen
HC
Lin
Ta-Hsien
TH
Huang
Hsien-bin
HB
D000199:Actins / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D004285:Dogs; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007962:Leukocytes / Q000737:chemistry; D010749:Phosphoprotein Phosphatases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D054645:Protein Phosphatase 1; D020798:Two-Hybrid System Techniques
D000199:Actins; C520010:PPP1R18 protein, human; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1