title: Single nucleotide polymorphisms in chicken lmbr1 gene were associated with chicken growth and carcass traits.
abstract: Lmbr1 is the key candidate gene controlling vertebrate limb development, but its effects on animal growth and carcass traits have never been reported. In this experiment, lmbr1 was taken as the candidate gene affecting chicken growth and carcass traits. T/C and G/A mutations located in exon 16 and one A/C mutation located in intron 5 of chicken lmbr1 were detected from Silky, White Plymouth Rock broilers and their F2 crossing chickens by PCR-SSCP and sequencing methods. The analysis of variance (ANOVA) results suggests that T/C polymorphism of exon 16 had significant association with eviscerated yield rate (EYR), gizzard rate (GR), shank and claw rate (SCR) and shank girth (SG); A/C polymorphism of intron 5 was significantly associated with SCR, liver rate and head-neck weight (HNW), while both sites had no significant association with other growth and carcass traits. These results demonstrate that lmbr1 gene could be a genetic locus or linked to a major gene significantly affecting these growth and carcass traits in chicken.
authors: State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, 100094, China.
authors: YanQun
authors: Y
authors: Huang
authors: XiaoHui
authors: X
authors: Du
authors: XueMei
authors: X
authors: Deng
authors: XiangPin
authors: X
authors: Qiu
authors: ChunKao
authors: C
authors: Wang
authors: Wen
authors: W
authors: Chen
authors: Ning
authors: N
authors: Li
authors: ChangXin
authors: C
authors: Wu
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000235:genetics* / Q000502:physiology; D001824:Body Constitution / Q000235:genetics*; D002645:Chickens / Q000235:genetics* / Q000254:growth & development*; D008565:Membrane Proteins / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide
chemical_list: D030161:Avian Proteins; D008565:Membrane Proteins