[Evaluation of two techniques of molecular subtyping to study Pasteurella multocida].
Typeability, reproducibility, and discriminatory power of ERIC-PCR and Apal-PFGE to establish the genetic relation of P. multocida strains were determined. Forty-nine strains of different source, biotype, capsular group, somatic serotype, and resistance to antimicrobials were studied. By ERIC-PCR, 31 patterns were defined with 10 to 14 bands in a rank of 0.2 and 1.2 kb. By Apal-PFGE, 37 restriction patterns were established with 7 to 15 bands of 34 to 450 kb. Typeability was 100% (T=1) for ERIC-PCR, and 94% (T = 0.94) for Apal-PFGE. Reproducibility of both techniques was 100% (R=1). Discriminatory power was 93% (D = 0.93) for ERIC-PCR, and 98% (D = 0.98) for Apal-PFGE. By using both techniques, epidemiologically related strains were grouped, and unrelated strains were clearly differentiated. The value of ERIC-PCR and Apal-PFGE as complements to epidemiologic studies was demonstrated, especially when both techniques were used to analyze the strains.
Leotta
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GA
Servicio Fisiopatogenia,Departamento Bacteriología, Instituto Nacional de Enfermedades Infecciosas--Administracidó Nacional de Laboratorios e Institutos de Salud Dr. Carlos G. Malbrin, Buenos Aires, Argentina. galeotta@anlis.gov.ar
Chinen
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I
Vigo
G B
GB
Gugliada
J
J
Rivas
M
M
D000569:Americas; D000818:Animals; D000864:Antarctic Regions; D001315:Australia; D001715:Bird Diseases / Q000382:microbiology; D001717:Birds / Q000382:microbiology; D002417:Cattle / Q000382:microbiology; D002418:Cattle Diseases / Q000382:microbiology; D002645:Chickens / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific; D024881:Drug Resistance, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D006801:Humans; D010326:Pasteurella Infections / Q000382:microbiology / Q000662:veterinary; D016979:Pasteurella multocida / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D011201:Poultry Diseases / Q000382:microbiology; D015203:Reproducibility of Results; D013552:Swine / Q000382:microbiology; D013553:Swine Diseases / Q000382:microbiology; D014422:Turkeys / Q000382:microbiology
D004269:DNA, Bacterial; D015252:Deoxyribonucleases, Type II Site-Specific; C038485:GGGCCC-specific type II deoxyribonucleases