title: Contribution of insulin-like growth factor I to the healing of colonic anastomoses in rats.
abstract: The aim of this experimental study was to investigate the contribution of insulin-like growth factor I (IGF)-I in the colonic healing process when injected intraperitoneally after colon resection. Forty male Wistar rats were used. Rats in the control group were injected with 3 mL of a solution of 0.9% NaCl intraperitoneally after the operation and on postoperative day 2, 4, and 6. Rats in the IGF-I group received recombinant human IGF-I in a dose of 2 mg/kg body weight intraperitoneally, immediately after the colonic anastomosis was performed and on postoperative day 2, 4, and 6. All rats were killed on postoperative day 7. The hydroxyproline tissue content was significantly higher in the IGF-1 group than in the control group. The bursting pressures were also significantly higher in IGF-1 group than in the control group. The weight change between the groups differed significantly; in the control group the average weight decreased about 5% postoperatively, while in the IGF-1 group the average weight increased about 6%. The average inflammatory cell infiltration score was significantly higher in the control group. Neoagiogenesis did not differ significantly between the two groups. The fibroblast activity differed significantly between the two groups, as the control group had significantly less fibroblasts compared to the IGF-1 group. In conclusion, IGF-I when given intraperitoneally stimulates the healing of colonic anastomoses in the rats. Further studies are required in order to determine whether this effect is dose related.
authors: 4th Surgical Department, Aristotle University of Thessaloniki, G. Papanikolaou General Hospital, Exohi, Thessaloniki 57010. Greece. manoszacharakis@hotmail.com
authors: Emmanouil
authors: E
authors: Zacharakis
authors: Haralampos
authors: H
authors: Demetriades
authors: Dimitrios
authors: D
authors: Kanellos
authors: Nikolaos
authors: N
authors: Sapidis
authors: Evangelos
authors: E
authors: Zacharakis
authors: Ioannis
authors: I
authors: Mantzoros
authors: Ioannis
authors: I
authors: Kanellos
authors: Georgios
authors: G
authors: Koliakos
authors: Thomas
authors: T
authors: Zaraboukas
authors: Konstantina
authors: K
authors: Topouridou
authors: Dimitrios
authors: D
authors: Betsis
mesh_terms: D000038:Abscess / Q000209:etiology; D000714:Anastomosis, Surgical; D000818:Animals; D001835:Body Weight; D002452:Cell Count; D003094:Collagen / Q000032:analysis; D003106:Colon / Q000737:chemistry / Q000187:drug effects / Q000473:pathology / Q000601:surgery*; D004353:Drug Evaluation, Preclinical; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology; D006909:Hydroxyproline / Q000032:analysis; D007249:Inflammation; D007274:Injections, Intraperitoneal; D007334:Insulin-Like Growth Factor I / Q000008:administration & dosage / Q000235:genetics / Q000627:therapeutic use*; D007421:Intestine, Small / Q000473:pathology; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects; D009852:Omentum / Q000473:pathology; D011183:Postoperative Complications / Q000209:etiology / Q000517:prevention & control; D011312:Pressure; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000627:therapeutic use; D016037:Single-Blind Method; D013530:Surgical Wound Infection / Q000209:etiology; D013718:Tensile Strength; D000267:Tissue Adhesions / Q000209:etiology; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D011994:Recombinant Proteins; D007334:Insulin-Like Growth Factor I; D003094:Collagen; D006909:Hydroxyproline