Carbamylated albumin is a potent inhibitor of polymorphonuclear neutrophil respiratory burst.
Carbamylation is a post-translational modification of proteins characterized by the binding of cyanate to amino groups, increased in renal failure. Pathophysiological consequences of carbamylation and adverse effects of carbamylated proteins on cell functions are poorly understood. We studied the influence of carbamylated albumin on polymorphonuclear neutrophil (PMN) O(2)(-) production. Carbamylated albumin significantly decreased O(2)(-) production in PMNs stimulated by type I collagen, but not by phorbol 12-myristate 13-acetate or tumor necrosis factor-alpha. This effect was related to inhibition of p(125)FAK phosphorylation. Such an alteration of neutrophil oxidative functions might explain characteristic complications of renal failure, such as increased occurrence of inflammation or infections.
Jaisson
Stéphane
S
Laboratory of Biochemistry and Molecular Biology, CNRS UMR 6198, Faculty of Medicine, University of Reims Champagne Ardenne, 51, rue Cognacq Jay, CNRS, F-51095 Reims Cedex, France. sjaisson@chu-reims.fr
Delevallée-Forte
Céline
C
Touré
Fatouma
F
Rieu
Philippe
P
Garnotel
Roselyne
R
Gillery
Philippe
P
D000818:Animals; D002219:Carbamates / Q000494:pharmacology*; D051417:Focal Adhesion Kinase 1 / Q000378:metabolism; D006801:Humans; D009504:Neutrophils / Q000187:drug effects*; D010100:Oxygen / Q000032:analysis / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D016897:Respiratory Burst / Q000187:drug effects*; D012709:Serum Albumin / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000031:analogs & derivatives / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D002219:Carbamates; D012709:Serum Albumin; D014409:Tumor Necrosis Factor-alpha; C016658:phorbolol myristate acetate; D051417:Focal Adhesion Kinase 1; C496050:PTK2 protein, human; D013755:Tetradecanoylphorbol Acetate; D010100:Oxygen