Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir.
The complete genome sequence of Geobacillus thermodenitrificans NG80-2, a thermophilic bacillus isolated from a deep oil reservoir in Northern China, consists of a 3,550,319-bp chromosome and a 57,693-bp plasmid. The genome reveals that NG80-2 is well equipped for adaptation into a wide variety of environmental niches, including oil reservoirs, by possessing genes for utilization of a broad range of energy sources, genes encoding various transporters for efficient nutrient uptake and detoxification, and genes for a flexible respiration system including an aerobic branch comprising five terminal oxidases and an anaerobic branch comprising a complete denitrification pathway for quick response to dissolved oxygen fluctuation. The identification of a nitrous oxide reductase gene has not been previously described in Gram-positive bacteria. The proteome further reveals the presence of a long-chain alkane degradation pathway; and the function of the key enzyme in the pathway, the long-chain alkane monooxygenase LadA, is confirmed by in vivo and in vitro experiments. The thermophilic soluble monomeric LadA is an ideal candidate for treatment of environmental oil pollutions and biosynthesis of complex molecules.
Feng
Lu
L
TEDA School of Biological Sciences and Biotechnology, Nankai University, Tianjin 300457, China.
Wang
Wei
W
Cheng
Jiansong
J
Ren
Yi
Y
Zhao
Guang
G
Gao
Chunxu
C
Tang
Yun
Y
Liu
Xueqian
X
Han
Weiqing
W
Peng
Xia
X
Liu
Rulin
R
Wang
Lei
L
D001406:Bacillaceae / Q000235:genetics*; D002633:Chemotaxis; D002681:China; D016680:Genome, Bacterial; D023281:Genomics / Q000379:methods*; D006899:Mixed Function Oxygenases / Q000235:genetics; D008954:Models, Biological; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009821:Oils; D016366:Open Reading Frames; D010088:Oxidoreductases / Q000235:genetics; D010101:Oxygen Consumption; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction
D009821:Oils; D006899:Mixed Function Oxygenases; D010088:Oxidoreductases; C022763:nitrous oxide reductase