Identification of trf2 mutants of Drosophila with defects in anterior spiracle eversion.
TATA-box-binding protein (TBP)-related factor (Trf)2 is a member of the family of TBP-related factors present in metazoan organisms. In Drosophila, Trf2 immunoprecipitates with the nucleosome remodeling factor (NURF) chromatin remodeling complex and the DNA replication element (DRE)-binding factor DREF. When it forms a complex with DREF, Trf2 activates transcription from the DRE-binding sites of the proliferating cell nuclear antigen (PCNA) gene. Despite these observations at the molecular level, no mutations in the trf2 locus have been found in Drosophila. Here, we identify two P-element insertion alleles, PL28 and GS7403, as hypomorphic mutants with a decreased expression level of trf2. Pupae of these mutant alleles show failure in anterior spiracle eversion, a hallmark of mutations in the loci associated with ecdysteroid signaling.
Shima
Seigo
S
Waseda University School of Human Sciences, Saitama, Japan.
Aigaki
Toshiro
T
Nojima
Tetsuya
T
Yamamoto
Daisuke
D
D000818:Animals; D017931:DNA Primers; D004251:DNA Transposable Elements / Q000235:genetics*; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D009154:Mutation / Q000235:genetics*; D010641:Phenotype; D015183:Restriction Mapping; D020133:Reverse Transcriptase Polymerase Chain Reaction; D035341:Telomeric Repeat Binding Protein 2 / Q000235:genetics* / Q000378:metabolism
D017931:DNA Primers; D004251:DNA Transposable Elements; D029721:Drosophila Proteins; C509085:TRF2 protein, Drosophila; D035341:Telomeric Repeat Binding Protein 2