Effect of human mesenchymal stem cell transplantation combined with growth factor infusion in the repair of injured spinal cord.
Recently, bone marrow stromal cells have been shown to have the capacity to differentiate into neural cell under experimental cell culture conditions. Some investigators suppose that these cells, when placed into an environment of injury, express factors that promote repair or active compensatory mechanisms and endogeneous stem cells within the injured tissue. Rats were subjected to a weight driven implant spinal cord injury. After one week, the rats were treated with cultured human mesenchymal stem cells (MSCs) transplantation and basic fibroblast growth factor (bFGF) infusion into the CSF space. Functional outcome and histologic evaluation were performed. The data showed improved functional outcome in the group treated with MSCs transplantation and bFGF administration compared with the group of MSCs transplantation and control, which means bFGF might take an additional role to improve functional outcome. Glial differentiation of MSCs was noted but neuronal differentiation was doubtful. In this study, we did not demonstrate the mechanism of the neurotrophic factor affecting neural repair. However, this study is consistent with growing literature that MSCs and neurotrophic factor promote tissue repair and functional recovery after spinal cord injury and suggests that MSCs transplantation and bFGF warrants investigation as a therapeutic intervention after spinal cord injury.
Kim
K N
KN
Department of Neurosurgery, Yonsei University College of Medicine, Seoul, Korea.
Oh
S H
SH
Lee
K H
KH
Yoon
D H
DH
D000818:Animals; D001812:Blood-Brain Barrier; D004195:Disease Models, Animal; D006133:Growth Substances / Q000008:administration & dosage / Q000627:therapeutic use*; D006801:Humans; D007262:Infusions, Intravenous; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000473:pathology / Q000628:therapy*; D014183:Transplantation, Heterologous
D006133:Growth Substances