title: [Detection of Mycobacterium tuberculosis DNA in sarcoidosis samples using real-time fluorescence polymerase chain reaction].
abstract: OBJECTIVE
  To study the role of Mycobacterium tuberculosis in the pathogenesis of sarcoidosis.
  
  
  METHODS
  Archival material of 22 patients with a histologic diagnosis of sarcoidosis were retrieved. Real-time fluorescent polymerase chain reaction (PCR) was used to detect DNA fragments of the complex-specific insertion sequence IS6110 of Mycobacterium tuberculosis in formalin-fixed and paraffin-embedded biopsy samples.
  
  
  RESULTS
  Among the 22 samples studied, Mycobacterium tuberculosis DNA was detected in 11 cases. The sequence of PCR amplified IS6110 DNA fragments completely matched with the related sequence in Mycobacterium tuberculosis gene.
  
  
  CONCLUSIONS
  Mycobacterium tuberculosis DNA is identified in a certain proportion of patients with a clinicopathologic diagnosis of sarcoidosis. Mycobacterium tuberculosis may be an important etiologic agent, at least in some of these patients.
authors: Department of Pathology, Tianjin Chest Hospital, Tianjin 300051, China.
authors: Mei-lin
authors: ML
authors: Xu
authors: Hao-hao
authors: HH
authors: Zhong
authors: Wan-jie
authors: WJ
authors: Heng
authors: Bing-quan
authors: BQ
authors: Wu
mesh_terms: D000328:Adult; D004269:DNA, Bacterial / Q000032:analysis*; D005260:Female; D005453:Fluorescence; D005500:Follow-Up Studies; D006801:Humans; D008198:Lymph Nodes / Q000382:microbiology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction / Q000379:methods*; D012507:Sarcoidosis / Q000382:microbiology* / Q000473:pathology
chemical_list: D004269:DNA, Bacterial