Facile preparation of glyconanoparticles and their bioconjugation to streptavidin.
Well-defined glycopolymers containing linear and cyclic carbohydrate moieties as pendent groups were prepared by reversible addition fragmentation chain transfer polymerization (RAFT). The RAFT synthesized glycopolymers were used for the aqueous synthesis of stabilized glyconanoparticles. The in situ reduction of the glycopolymers and HAuCl4 resulted in the formation of highly stable modified gold nanoparticles with diameters ranging from 40 to 80 nm in aqueous media. Multifunctional glyconanoparticles were also generated in the presence of varying amounts of biotinylated-polyethyleneglycol (bio-PEG-SH) having terminal thiol groups. The gold nanoparticles underwent aggregation in the presence of streptavidin as revealed by UV-vis spectroscopy. The availability of the biotin for conjugation to streptavidin was also confirmed using surface plasmon resonance (SPR).
Housni
Abdelghani
A
Department of Chemistry and Biochemistry, Biomolecular Sciences Program, Laurentian University, Sudbury, Ontario P3E 2C6, Canada.
Cai
Haijun
H
Liu
Shiyong
S
Pun
Suzie H
SH
Narain
Ravin
R
D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000138:chemical synthesis* / Q000737:chemistry; D006001:Glycoconjugates / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D019809:Streptavidin / Q000737:chemistry*; D013499:Surface Properties; D013997:Time Factors
D002241:Carbohydrates; D006001:Glycoconjugates; D006046:Gold; D019809:Streptavidin