[Study on kinetics of two-stage cultivation of protocorm-like bodies from Dendrobium huoshanense for cell growth and synthesis of polysaccharides].
Phosphate is an effective factor in the regulation of cell growth and accumulation of polysaccharides. The method for higher polysaccharide production has been obtained through feeding cultivation. The two-stage cultivation was proposed according to the characteristic of cell growth and the accumulation of polysaccharides in the suspension cultures of protocorm-like bodies from Dendrobium huoshanense. A kinetic model was developed to describe the cultivation process. The results indicate that the production of biomass and polysaccharides increases from 28.7g DW/L and 1.86g/L to 44.2g DW/L and 5.22g/L, respectively, and the content of polysaccharides increases from 6.4% to 11.9% . With the evaluated model parameters, the model appears to provide a description for the cultivation process.
Wei
Ming
M
School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China.
Jiang
Shao-Tong
ST
Luo
Jian-Ping
JP
D000465:Algorithms; D018533:Biomass; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002478:Cells, Cultured; D031667:Dendrobium / Q000166:cytology* / Q000378:metabolism*; D007700:Kinetics; D008954:Models, Biological; D018547:Plant Stems / Q000166:cytology / Q000378:metabolism; D011134:Polysaccharides / Q000096:biosynthesis*; D013997:Time Factors
D011134:Polysaccharides