[Bacterial flora analysis of sediment samples from Doukai Bay in Kitakyushu City].
The bacterial flora of 8 sediment samples taken from Doukai Bay in Kitakyushu City in May, 2006 was analyzed by using the culture-independent molecular method. The total bacterial numbers determined by epifluorescence microscopic method were in the range of 2.6 x 10(8) to 6.4 x 10(8) cells/g. The results of bacterial flora analysis suggested that unknown bacterium comprised over 80% of total bacterial flora in the sea sediment samples. Regarding the pathogenic bacteria, although 8 of the Vibrio species, 1 of the Staphylococcus species, and 2 of the Mycobacterium species were detected in the sediment samples, their frequency was low. A large proportion of bacteria related to sulfur circulation, such as sulfur-oxidizing bacteria (10.9 to approximately 30.5%) and sulfate-reducing bacteria (19.6 to approximately 25.1%), was detected in all of the samples from sea sediment. A remarkable difference in bacterial flora between ground soil and sea sediment was clarified by this experiment.
Fukuda
Kazumasa
K
Department of Microbiology, School of Medicine, University of Occupational and Environmental Health, Yahatanishi-ku, Kitakyushu, Japan.
Ichihara
Tsuyoshi
T
Ueda
Naoko
N
Koriyama
Kazuaki
K
Ogawa
Midori
M
Taniguchi
Hatsumi
H
D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D015169:Colony Count, Microbial; D019015:Geologic Sediments / Q000382:microbiology*; D007564:Japan; D009161:Mycobacterium / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S; D013210:Staphylococcus / Q000235:genetics / Q000302:isolation & purification; D014733:Vibrio / Q000235:genetics / Q000302:isolation & purification
D012336:RNA, Ribosomal, 16S