title: Suppression subtractive hybridization identifies novel transcripts in regenerating Hydra littoralis.
abstract: Despite considerable interest in the biologic processes of regeneration and stem cell activation, little is known about the genes involved in these transformative events. In a Hydra littoralis model of regeneration, we employed a rapid shotgun suppression subtractive hybridization strategy to identify genes that are uniquely expressed in regenerating tissue. With an adaptor-PCR based technique, 16 candidate transcripts were identified, 15 were confirmed unique to mRNA isolated from hydra undergoing regeneration. Of these, 6 were undescribed in GenBank and allied expressed sequence tag (EST) databases (GenBank + EMBL + DDBJ + PDB and the Hydra EST database). BLAST analysis of these sequences identified remarkably similar sequences in anonymous ESTs found in a wide variety of animal species.
authors: Casey Eye Institute, Oregon Health and Science University, 3375 SW Terwilliger Blvd, Portland, OR 97239, USA.
authors: Thomas
authors: T
authors: Stout
authors: Trevor
authors: T
authors: McFarland
authors: Binoy
authors: B
authors: Appukuttan
mesh_terms: D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D020224:Expressed Sequence Tags; D006829:Hydra / Q000235:genetics* / Q000502:physiology*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012038:Regeneration
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger