Clinical usefulness of serum EBV DNA levels of BamHI W and LMP1 for Nasal NK/T-cell lymphoma.
Quantitative real-time polymerase chain reaction (PCR) was utilized to measure serum EBV DNA levels of BamHI W fragment and latent membrane protein 1 (LMP1) in 20 nasal natural killer (NK)/T-cell lymphoma patients. Both serum EBV DNAs were detected at high levels in all patients, but the levels were below the limit of detection in all healthy controls. The BamHI Z fragment, Epstein-Barr-replication activator (ZEBRA) expression was detected in a small proportion (0.1-3%) of lymphoma cells from 10 (50%) of the patients. Patients with ZEBRA expression showed significantly higher DNA levels of BamHI W and LMP1 (P = 0.0081, P = 0.004), suggesting that EBV DNA may be caused by EBV replication from lymphoma cells. Kaplan-Meier and univariate analyses revealed that high DNA levels of BamHI W and LMP1 at pre-treatment and high BamHI W DNA level at post-treatment were associated with short disease-free survival and overall survival (P < 0.05, each). Although the DNA levels of BamHI W and LMP1 correlated significantly, their dynamics were not always parallel. Patients with low pre-treatment level of both EBV DNAs showed a favorable course, in contrast to patients with high pre-treatment level of both EBV DNAs who showed an aggressive course (P = 0.0085). More importantly, the high pre-treatment level of both EBV DNAs was determined as the only independent prognostic factor among various prognostic factors. These data suggest that simultaneous measurement of serum levels of both BamHI W and LMP1 DNAs may be useful for diagnosis, disease monitoring, and prediction of prognosis for nasal NK/T-cell lymphoma patients.
Ishii
Hideyuki
H
Department of Otolaryngology-Head and Neck Surgery, Asahikawa Medical College, Asahikawa, Japan.
Ogino
Takeshi
T
Berger
Christoph
C
Köchli-Schmitz
Nicole
N
Nagato
Toshihiro
T
Takahara
Miki
M
Nadal
David
D
Harabuchi
Yasuaki
Y
D000328:Adult; D000368:Aged; D000956:Antigens, Viral; D015415:Biomarkers / Q000097:blood; D017931:DNA Primers; D004279:DNA, Viral / Q000097:blood*; D015245:Deoxyribonuclease BamHI / Q000235:genetics*; D005260:Female; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007694:Killer Cells, Natural; D016399:Lymphoma, T-Cell / Q000097:blood / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D009669:Nose Neoplasms / Q000097:blood / Q000175:diagnosis*; D010446:Peptide Fragments / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D011379:Prognosis; D014763:Viral Matrix Proteins / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
D000956:Antigens, Viral; D015415:Biomarkers; D017931:DNA Primers; D004279:DNA, Viral; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D010446:Peptide Fragments; D014763:Viral Matrix Proteins; D014764:Viral Proteins; D015245:Deoxyribonuclease BamHI