Validation and comparison of the Copan Milk Test and Delvotest SP-NT for the detection of antimicrobials in milk.
The Delvotest SP-NT and Copan Milk Test, two microbiological tests designed for screening antimicrobial substances in milk were compared and validated. The performance criteria described by the European Decision 2002/657/EC were used for the study. Both tests were evaluated with visual and automated reading (scanner) and the validation was performed on 10 different antibiotics (penicillin-G, cloxacillin, sulfamethazine, sulfadiazine, oxytetracycline, gentamicin, cephalexin, cefquinome, dihydrostreptomycin and trimethoprim). Both tests were found to detect penicillin, cloxacillin, sulfamethazine, sulfadiazine, cephalexin and gentamicin at or below the EU maximum residue limits (MRLs). Some other antibiotics such as oxytetracycline, dihydrostreptomycin, trimethoprim and cefquinome were not detected or only with a low sensitivity. Both tests were found easy to use, robust and fulfilled EU requirements.
Le Breton
Marie-Hélène
MH
Nestlé Research Center, Nestlé Ltd., P.O. Box 44, CH-1000 Lausanne 26, Switzerland. marie-helene.le-breton@rdls.nestle.com
Savoy-Perroud
Marie-Claude
MC
Diserens
Jean-Marc
JM
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry; D000890:Anti-Infective Agents / Q000032:analysis* / Q000737:chemistry; D002417:Cattle; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation / Q000379:methods; D004350:Drug Residues / Q000032:analysis; D005504:Food Analysis / Q000295:instrumentation / Q000379:methods*; D008892:Milk / Q000378:metabolism*; D015203:Reproducibility of Results; D012107:Research Design; D012680:Sensitivity and Specificity
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents