Formation of an enolate intermediate is required for the reaction catalyzed by 3-hydroxyacyl-CoA dehydrogenase.
Fluorinated substrate analogs were synthesized and incubated with rat liver 3-hydroxyacyl-CoA dehydrogenase, which reveals that the formation of an enolate intermediate is required for the reaction catalyzed by the enzyme.
Liu
Xiaojun
X
Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, PR China.
Deng
Guisheng
G
Chu
Xiusheng
X
Li
Nan
N
Wu
Long
L
Li
Ding
D
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000737:chemistry* / Q000378:metabolism*; D000214:Acyl Coenzyme A / Q000737:chemistry*; D000818:Animals; D002384:Catalysis; D003065:Coenzyme A / Q000138:chemical synthesis / Q000737:chemistry*; D051381:Rats; D013379:Substrate Specificity
D000214:Acyl Coenzyme A; C010667:acetoacetyl CoA; D015094:3-Hydroxyacyl CoA Dehydrogenases; D003065:Coenzyme A