Expression and initial characterization of human ALDH3B1.
Aldehyde dehydrogenases (ALDHs) are critical enzymes in the metabolism of endogenous and exogenous aldehydes. The human genome contains 19 putatively functional ALDH genes; ALDH3B1 belongs to the ALDH3 family. While recent studies have linked the ALDH3B1 locus to schizophrenia, nothing was known, until now, about the properties and significance of the ALDH3B1 protein. The aim of this study was to characterize the ALDH3B1 protein. Human ALDH3B1 was baculovirus-expressed and found to be catalytically active towards medium- and long-chain aliphatic aldehydes and the aromatic aldehyde benzaldehyde. Western blot analyses indicate that ALDH3B1 is highly expressed in kidney and liver and moderately expressed in various brain regions. ALDH3B1-transfected HEK293 cells were significantly protected against cytotoxicity induced by the lipid peroxidation product octanal when compared to vector-transfected cells. This study shows for the first time the functionality, expression and protective role of ALDH3B1 and indicates a potential physiological role of ALDH3B1 against oxidative stress.
Marchitti
Satori A
SA
Molecular Toxicology & Environmental Health Sciences Program, Department of Pharmaceutical Sciences, University of Colorado Health Sciences Center, Denver, CO, USA.
Orlicky
David J
DJ
Vasiliou
Vasilis
V
D000444:Aldehyde Dehydrogenase / Q000096:biosynthesis* / Q000737:chemistry* / Q000378:metabolism; D000445:Aldehyde Oxidoreductases / Q000096:biosynthesis* / Q000737:chemistry* / Q000378:metabolism; D000447:Aldehydes / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D006801:Humans; D007668:Kidney / Q000201:enzymology; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D014018:Tissue Distribution
D000447:Aldehydes; C030863:corneal protein 54, bovine; D000445:Aldehyde Oxidoreductases; D000444:Aldehyde Dehydrogenase; C520012:ALDH3B1 protein, human; C031639:caprylic aldehyde