Comparison of matrix solid-phase dispersion and liquid-liquid extraction for the chromatographic determination of fenthion and its metabolites in olives and olive oils.
Matrix solid-phase dispersion (MSPD) methodology has been developed to extract fenthion and its metabolites from olives and olive oils, and the technique compared with conventional liquid-liquid extraction (LLE). The method was applied to olives and olive oil samples obtained from olive groves treated with fenthion. Pesticide residues were analysed by gas chromatography (GC) using a nitrogen-phosphorus detector. Both extraction methods gave a linear response over the concentration range assayed (0.03-1 mg kg-1 for MSPD and 0.1-1 mg kg-1 for LLE). Recoveries and RSD (n = 6) values in olives were 85-112% and 2-6% for MSPD, and 68-108% and 4-16% for LLE, respectively. In the case of olive oil, recoveries and RSD (n = 6) values were 67-98% and 5-11% for MSPD, and 63-115% and 6-14% for LLE, respectively. When compared to LLE, the newly developed MSPD method was twice as sensitive and required 10 times less sample weight.
Cunha
Sara C
SC
REQUIMTE/Serviço de Bromatologia, Faculdade de Farmácia, Universidade do Porto. Rua Aníbal Cunha, 164, 4099-030 Porto, Portugal. sara.cunha@ff.up.pt
Fernandes
José O
JO
Beatriz
M
M
Oliveira
P P
PP
D002845:Chromatography / Q000379:methods* / Q000592:standards; D005284:Fenthion / Q000032:analysis* / Q000302:isolation & purification; D005504:Food Analysis; D005506:Food Contamination; D007306:Insecticides / Q000032:analysis*; D031658:Olea / Q000737:chemistry*; D000069463:Olive Oil; D010573:Pesticide Residues / Q000032:analysis* / Q000302:isolation & purification; D010938:Plant Oils / Q000032:analysis*
D007306:Insecticides; D000069463:Olive Oil; D010573:Pesticide Residues; D010938:Plant Oils; D005284:Fenthion