Potential side effects of dendritic cells pulsed with allogenic melanoma cell lysate in mice.
An attempt has been made to investigate the toxicity of cancer immunotherapy based on the dendritic cells pulsed with lysate of allogenic melanoma cell, DM401. Dendritic cells pulsed with lysate of clone M3 were subcutaneously administered once a week eight times to C57BL/6 mice at 0, 2.5, 5, and 10 x 10(7) cells/kg. No changes attributable to the administration were observed in clinical signs and food and water consumption. The administration induced slight increases in body weights, white blood cells, total protein, total cholesterol, triglyceride, phospholipids, and absolute spleen weights, but a slight decrease in albumin/globulin ratio. Microscopic examinations revealed the infiltration of inflammatory cells in the lung, mainly in the pulmonary arteriole, in which the tunica media thickened, and in the pulmonary alveoli and alveolar space. Thickened tunica media of pulmonary arteriole was observed in both males and females at all selected doses. In addition, the subcutis at the test substance-application site showed inflammation and fibrosis. In conclusion, lung is a target organ of DM401, and most of the changes including the findings in lung are considered as the immunomodulatory functions of dendritic cells.
Park
Shin-Young
SY
Korea Institute of Toxicology, Daejeon, Korea.
Choi
Woo Hyuck
WH
Kim
Yong Bum
YB
Ha
Chang Su
CS
Lee
Hyunah
H
Kwon
Young-Do
YD
Choi
Ok-Mi
OM
Lee
Young-Joon
YJ
Koh
Woo Suk
WS
D000418:Albumins / Q000378:metabolism; D000818:Animals; D017690:Cell Transplantation; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D005916:Globulins / Q000378:metabolism; D007167:Immunotherapy / Q000379:methods; D007958:Leukocyte Count; D008168:Lung / Q000276:immunology* / Q000473:pathology; D008297:Male; D008545:Melanoma / Q000276:immunology* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL
D000418:Albumins; D005916:Globulins