Differential response of human healthy lymphoblastoid and CCRF-SB leukemia cells to sulforaphane and its two analogues: 2-oxohexyl isothiocyanate and alyssin.
The chemopreventive effect of sulforaphane and two of its analogues on human B-lymphocytes derived cells was evaluated in this study. Two cell lines used in the experiments were: human lymphoblastoid cells and human B-leukemia CCRF-SB. Both cell lines were treated with three structurally related isothiocyanates: sulforaphane, 2-oxohexyl isothiocyanate and alyssin. The viability of cells, induction of a phase II enzyme-quinone reductase, apoptosis induction, GSH content and ROS formation were evaluated. The results indicate the differences between the chemopreventive properties and apoptosis-inducing activity of three isothiocyanates. The significant differences in response to these compounds were observed between healthy lymphoblastoid and leukemia CCRF-SB cells.
Misiewicz
Irena
I
Confocal Microscopy Laboratory, National Institute of Public Health, Chełmska 30/34, PL 00-725 Warszawa, Poland.
Skupinska
Katarzyna
K
Kasprzycka-Guttman
Teresa
T
D016588:Anticarcinogenic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001402:B-Lymphocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D017879:Isothiocyanates; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013329:Structure-Activity Relationship; D013454:Sulfoxides; D013861:Thiocyanates / Q000737:chemistry / Q000494:pharmacology*
C524315:2-oxohexyl isothiocyanate; D016588:Anticarcinogenic Agents; D017879:Isothiocyanates; D017382:Reactive Oxygen Species; D013454:Sulfoxides; D013861:Thiocyanates; C524316:alyssin; D016660:NAD(P)H Dehydrogenase (Quinone); C016766:sulforaphane; D005978:Glutathione