Ewing Sarcoma tumor cells express CD34: implications for autologous stem cell transplantation.
The significance of tumor cell contamination in marrow and peripheral blood stem cell (PBSC) collections of patients with solid tumors remains controversial. Various methods have been developed to purge tumor cells from autologous stem cell products, including CD34+ selection. PBSC harvests from patients with Ewing family of tumors (EFT) were analyzed for contaminating tumor cells prior and after CD34+ selection using reverse transcription-polymerase chain reaction (RT-PCR) and flow cytometry (FC) analyzes. The expression of CD34 was studied by RT-PCR and FC in 14 primary tumors and 13 PBSC harvests, respectively. Tumor cells were identified in the harvests by both methods. In two patients, contaminating tumor cells were evident by RT-PCR only after positive selection. FC analysis confirmed a higher level of tumor cells in the CD34+ fraction. In an attempt to explore this finding, expression of CD34 was detected in 93% of primary tumors and 67% of contaminated harvests. As CD34 is expressed on EFT cells, these cells may be enriched following CD34+ selection of harvests, although the total number of tumor cells is reduced. Other methods of purging, rather than CD34+ selection, should be explored in patients with EFT undergoing autologous stem cell transplantation.
Yaniv
I
I
Pediatric Hematology Oncology, Schneider Children's Medical Center of Israel, Petah Tikva 49202, Israel.
Stein
J
J
Luria
D
D
Cohen
I J
IJ
Liberzon
E
E
Manor
S
S
Grunshpan
A
A
Sverdlov
Y
Y
Kodman
Y
Y
Issakov
J
J
Feinmesser
M
M
Zaizov
R
R
Avigad
S
S
D000293:Adolescent; D000328:Adult; D018952:Antigens, CD34 / Q000378:metabolism*; D002469:Cell Separation; D002648:Child; D002675:Child, Preschool; D003131:Combined Modality Therapy; D005434:Flow Cytometry; D006801:Humans; D007223:Infant; D015514:Oncogene Proteins, Fusion / Q000235:genetics; D036102:Peripheral Blood Stem Cell Transplantation; D051789:Proto-Oncogene Protein c-fli-1 / Q000235:genetics; D034802:RNA-Binding Protein EWS; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012512:Sarcoma, Ewing / Q000235:genetics / Q000276:immunology* / Q000628:therapy*; D014182:Transplantation, Autologous
D018952:Antigens, CD34; C106031:EWS-FLI fusion protein; D015514:Oncogene Proteins, Fusion; D051789:Proto-Oncogene Protein c-fli-1; D034802:RNA-Binding Protein EWS