Leukoreduced buffy coat-derived platelet concentrates photochemically treated with amotosalen HCl and ultraviolet A light stored up to 7 days: assessment of hemostatic function under flow conditions.
BACKGROUND
Amotosalen plus ultraviolet A light photochemical treatment (PCT) inactivates high titers of bacteria, and other pathogens, in platelet concentrates (PCs) potentially allowing the storage of platelets (PLTs) for up to 7 days. Adhesion and aggregation of PLTs to injured vascular surfaces are critical aspects of PLT hemostatic function.


METHODS
Two ABO-identical leukoreduced buffy coat-derived PCs in additive solution were mixed and divided: one-half underwent PCT (PCT-PCs) and the other was kept as a control (C-PCs); both were stored under standard conditions. The total number of paired PCs studied was nine. Samples were taken on Day 1 (before PCT) and after 5 and 7 days of storage. The adhesion and aggregation capacities were evaluated under flow conditions in a ex vivo perfusion model.


RESULTS
Compared to control, PCT resulted in a decrease in PLT count of 6.5 percent (p = 0.004) and 10.2 percent (p = 0.008) after 5 and 7 days' storage, respectively (n = 9). PLT interaction with subendothelium was mainly in form of adhesion. The surface covered by PCT PLTs on Day 1 was 26.0 +/- 4.2 percent (mean +/- SEM). On Day 5, PCT-PCs showed a covered surface of 20.9 +/- 2.2 percent, and the C-PCs, 20.6 +/- 1.6 percent. After 7 days, PCT-PCs produced a nonsignificant higher PLT deposition compared to control (27.1 +/- 2.9% vs. 21.2 +/- 2.8%, p = 0.06).


CONCLUSIONS
PCT of PCs and storage up to 7 days was associated with a 10.2 percent decrease in PLT count due to processing losses compared to C-PC. PLT adhesive and aggregating capacities under flow conditions of PCT-PCs were similar to C-PCs and remained well preserved for up to 7 days of storage.
Lozano
Miguel
M
Department of Hemotherapy and Hemostasis, Hospital Clínic Provincial, University of Barcelona, Barcelona, Spain. mlozano@clinic.ub.es
Galan
Ana
A
Mazzara
Roberto
R
Corash
Laurence
L
Escolar
Ginés
G
D001792:Blood Platelets / Q000187:drug effects* / Q000528:radiation effects*; D001793:Blood Preservation / Q000379:methods; D011564:Furocoumarins / Q000494:pharmacology; D006487:Hemostasis / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D007958:Leukocyte Count; D010777:Photochemistry / Q000379:methods; D010974:Platelet Aggregation / Q000187:drug effects / Q000528:radiation effects; D010983:Plateletpheresis / Q000379:methods; D013997:Time Factors; D014466:Ultraviolet Rays
D011564:Furocoumarins; C118577:amotosalen