Callus of Securinega suffruticosa, a cell line accumulates dextro Securinega alkaloids.
Investigations on callus cultures of Securinega suffruticosa indicated that the cell line of S. suffruticosa callus was able to accumulate four known Securinega alkaloids with dextro rotation but not levo rotation as reported before: virosecurinine (1), viroallosecurinine (2), 14,15-dihydrovirosecurinine (3) and ent-phyllanthidine (4). Time course studies on the growth of callus cultures were carried out. The effects of different plant growth regulators, sucrose concentrations on callus growth and virosecurinine production were also reported.
Yuan
Wei
W
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
Zhu
Ping
P
Cheng
Kedi
K
Meng
Chao
C
Ma
Lin
L
Wu
Feng
F
Zhu
Huixin
H
D000470:Alkaloids / Q000032:analysis / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D001381:Azepines / Q000032:analysis; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D003583:Cytokinins / Q000494:pharmacology; D019665:Euphorbiaceae / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism*; D006576:Heterocyclic Compounds, 4 or More Rings / Q000032:analysis; D006572:Heterocyclic Compounds, Bridged-Ring; D007210:Indoleacetic Acids / Q000494:pharmacology; D007783:Lactones / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D010880:Piperidines / Q000032:analysis; D018547:Plant Stems / Q000166:cytology / Q000378:metabolism; D013237:Stereoisomerism
D000470:Alkaloids; D001381:Azepines; D003470:Culture Media; D003583:Cytokinins; D006576:Heterocyclic Compounds, 4 or More Rings; D006572:Heterocyclic Compounds, Bridged-Ring; D007210:Indoleacetic Acids; D007783:Lactones; D010880:Piperidines; C012607:virosecurinine; C000785:securinine