Oxytetracycline as environmental contaminant in arable lands.
Oxytetracycline (OXY) is a broad-range antimicrobial routinely used in pig production, at doses in the range of few g/kg of medicated feed, during the weaning period. It could persist at ppm level in pig liquid manure that routinely is used for organic fertilisation. In the present work we describe a methodology to study OXY environmental fate in arable land where crops are cultivated for animal feeding purposes. A liquid-liquid extraction followed by metal chelate affinity chromatography was applied to environmental samples of manures and soils drawn within a case-control study. Extracts were then analysed by high performance liquid chromatography with UV/DAD detection, using a reverse phase column, and expressing the results as 4-epioxytetracycline epimer. Results indicate OXY is well retained at mg kg(-1) levels in soil exposed to contaminated pig manure fertilisation. Such compartment could constitute an abiotic reservoir for the systemic and/or for the external contamination of corn.
Brambilla
Gianfranco
G
ISS Environment Department, Rome, Italy. g.brambi@iss.it
Patrizii
Michele
M
De Filippis
Stefania Paola
SP
Bonazzi
Giuseppe
G
Mantovi
Paolo
P
Barchi
Davide
D
Migliore
Luciana
L
D000818:Animals; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004784:Environmental Monitoring / Q000379:methods*; D005308:Fertilizers; D006863:Hydrogen-Ion Concentration; D008372:Manure; D010118:Oxytetracycline / Q000032:analysis*; D015203:Reproducibility of Results; D012987:Soil; D012989:Soil Pollutants / Q000032:analysis*; D013552:Swine; D013752:Tetracycline / Q000032:analysis; D003313:Zea mays / Q000378:metabolism
D005308:Fertilizers; D008372:Manure; D012987:Soil; D012989:Soil Pollutants; D013752:Tetracycline; D010118:Oxytetracycline