A fundamental regulatory role of formate on thuringiensin production by resting cell of Bacillus thuringiensis YBT-032.
In this work, a fundamental regulatory role of formate on thuringiensin production by resting cell of Bacillus thuringiensis YBT-032 was investigated. Nicotinamide adenine dinucleotide (NADH) production and formate dehydrogenase activity increased with formate addition from 0.5 to 2.0 g/L, respectively. However, with the formate addition of 1.5 g/L, the activities of pyruvate kinase and glucose 6-phosphate dehydrogenase reached a peak and increased by 316 and 150% relative to those of the control, respectively. In addition, intracellular production of pyruvate, aspartate, citrate and adenine were significantly enhanced by 75, 66, 32 and 78% as well. An improvement (90%) of thuringiensin production was also successfully obtained. Interestingly to point out, thuringiensin yield was closely correlative with adenine production, and the linear relationship was also observed. The results suggest that appropriate formate addition did act as a modulator and facilitate carbon flux in glycolysis and pentose phosphate pathway to synthesize adenine and thuringiensin via intracellular NADH availability.
Zhi
Wang
W
National Engineering Research Center for Microbial Pesticides, State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
Shouwen
Chen
C
Lifang
Ruan
R
Ming
Sun
S
Ziniu
Yu
Y
D000225:Adenine / Q000096:biosynthesis*; D000241:Adenosine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D001413:Bacillus thuringiensis / Q000187:drug effects / Q000378:metabolism*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005561:Formates / Q000008:administration & dosage*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects / Q000502:physiology*; D006019:Glycolysis / Q000187:drug effects; D010427:Pentose Phosphate Pathway / Q000187:drug effects / Q000502:physiology; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D013400:Sugar Acids
D005561:Formates; D013400:Sugar Acids; C030544:formic acid; D000225:Adenine; C068295:thuringiensin; D000241:Adenosine