title: Expression survey of genes critical for tooth development in the human embryonic tooth germ.
abstract: In the developing murine tooth, the expression patterns of numerous regulatory genes have been examined and their roles have begun to be revealed. To unveil the molecular mechanisms that regulate human tooth morphogenesis, we examined the expression patterns of several regulatory genes, including BMP4, FGF8, MSX1, PAX9, PITX2, and SHOX2, and compared them with that found in mice. All of these genes are known to play critical roles in murine tooth development. Our results show that these genes exhibit basically similar expression patterns in the human tooth germ compared with that in the mouse. However, slightly different expression patterns were also observed for some of the genes at certain stages. For example, MSX1 expression was detected in the inner enamel epithelium in addition to the dental mesenchyme at the bell stage of the human tooth. Moreover, FGF8 expression remained in the dental epithelium at the cap stage, while PAX9 and SHOX2 expression was detected in both dental epithelium and mesenchyme of the human tooth germ. Our results indicate that, although slight differences exist in the gene expression patterns, the human and mouse teeth not only share considerable homology in odontogenesis but also use similar underlying molecular networks.
authors: College of Life Sciences, Fujian Normal University, Fuzhou, Fujian, People's Republic of China.
authors: Dahe
authors: D
authors: Lin
authors: Yide
authors: Y
authors: Huang
authors: Fenglei
authors: F
authors: He
authors: Shuping
authors: S
authors: Gu
authors: Guozhong
authors: G
authors: Zhang
authors: YiPing
authors: Y
authors: Chen
authors: Yanding
authors: Y
authors: Zhang
mesh_terms: D000818:Animals; D001483:Base Sequence; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D051524:Fibroblast Growth Factor 8 / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization; D051957:MSX1 Transcription Factor / Q000235:genetics; D051379:Mice; D009805:Odontogenesis / Q000235:genetics*; D051764:PAX9 Transcription Factor / Q000235:genetics; D013045:Species Specificity; D014083:Tooth Germ / Q000196:embryology*; D014157:Transcription Factors / Q000235:genetics; D000097577:Homeobox Protein PITX2
chemical_list: D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins; D017931:DNA Primers; D051524:Fibroblast Growth Factor 8; D018398:Homeodomain Proteins; D051957:MSX1 Transcription Factor; D051764:PAX9 Transcription Factor; D014157:Transcription Factors; D000097577:Homeobox Protein PITX2; C525809:BMP4 protein, human; C525810:Bmp4 protein, mouse; C496456:FGF8 protein, human; C484083:MSX1 protein, human; C497323:PAX9 protein, human; C484664:SHOX2 protein, human