title: Structural confirmation of regioisomers of Lopinavir impurities using MS and gradient COSY (1H and 13C NMR assignment of Lopinavir impurities).
abstract: We report the complete (1)H and (13)C NMR assignment of impurities of six Lopinavir (2S)-N-[(2S, 4S, 5S)-5-{[2-(2,6-dimethylphenoxy)acetyl]amino}-4-hydroxy-1,6-diphenyl hexan-2-yl]-3-methyl-2-(2-oxo-1,3-diazinan-1-yl)butan- amide. Two of the impurities are regioisomers and GCOSY used to differentiate the two structures. The spectral assignments for all six impurities were achieved by concerted application of one and two-dimensional NMR techniques ((1)H NMR, (13)C NMR, DEPT, GCOSY, GHSQC and GHMBC).
authors: Analytical Research and Development, Matrix Laboratories Ltd., Jeedimetla, Hyderabad, India. sivalakshmi@matrixlabsindia.com
authors: A
authors: A
authors: Siva Lakshmi Devi
authors: Y
authors: Y
authors: Srinivasa Rao
authors: Y
authors: Y
authors: Suresh
authors: M
authors: M
authors: Yogeswar Reddy
authors: G
authors: G
authors: Jyothi
authors: B
authors: B
authors: Rajababu
authors: V S R
authors: VS
authors: Prasad
authors: V
authors: V
authors: Umamaheswar Rao
mesh_terms: D017320:HIV Protease Inhibitors / Q000737:chemistry*; D007536:Isomerism; D061466:Lopinavir; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure; D011744:Pyrimidinones / Q000737:chemistry*; D012015:Reference Standards
chemical_list: D017320:HIV Protease Inhibitors; D011744:Pyrimidinones; D061466:Lopinavir