title: Direct detection of human herpesvirus 6 DNA in serum by the loop-mediated isothermal amplification method.
abstract: BACKGROUND
  A more rapid and easier method is needed for monitoring human herpesvirus 6 (HHV-6) infections. The loop-mediated isothermal amplification method (LAMP) can detect viral DNA with high specificity, efficiency, and speed under isothermal conditions. LAMP requires only simple equipment that is available in hospital laboratories.
  
  
  OBJECTIVE
  We evaluated LAMP as a means of detecting HHV-6 DNA directly from patients' sera.
  
  
  RESULTS
  The sensitivity of the HHV-6 LAMP protocol without heat denaturation was 1000 copies/tube; with heat denaturation 10 copies/tube were detected. Three hundred serum samples from children with fever were analyzed. Using HHV-6 isolation as a definition of HHV-6 infection, the sensitivity, specificity, positive predictive value, and negative predictive value of the HHV-6 LAMP method without DNA extraction were 95.5%, 95.2%, 94.0%, and 96.4%, respectively.
  
  
  CONCLUSIONS
  Direct detection of HHV-6 DNA in serum with a modified HHV-6 LAMP could be used for rapid diagnosis of exanthem subitum (ES).
authors: Department of Medical Information Technology, Fujita Health University College, Toyoake, Aichi, Japan.
authors: Masaru
authors: M
authors: Ihira
authors: Shiho
authors: S
authors: Akimoto
authors: Fumi
authors: F
authors: Miyake
authors: Ayano
authors: A
authors: Fujita
authors: Ken
authors: K
authors: Sugata
authors: Sadao
authors: S
authors: Suga
authors: Masahiro
authors: M
authors: Ohashi
authors: Naoko
authors: N
authors: Nishimura
authors: Takao
authors: T
authors: Ozaki
authors: Yoshizo
authors: Y
authors: Asano
authors: Tetsushi
authors: T
authors: Yoshikawa
mesh_terms: D017931:DNA Primers; D004279:DNA, Viral / Q000097:blood*; D005077:Exanthema Subitum / Q000097:blood / Q000821:virology*; D005260:Female; D005334:Fever / Q000097:blood / Q000821:virology; D015654:Herpesvirus 6, Human / Q000235:genetics*; D006801:Humans; D007223:Infant; D008297:Male; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D004279:DNA, Viral