Electrochemical properties of cytochroms P450 using nanostructured electrodes: direct electron transfer and electro catalysis.
The present study demonstrates direct electron transfer between cytochromes P450 2B4 (CYP2B4), P450 1A2 (CYP1A2), sterol 14alpha-demethylase (CYP51b1) on the one hand and screen-printed graphite electrodes, modified with gold nanoparticles and didodecyldimethylammonium bromide (DDAB) on the other. Electro detection of heme proteins was possible when 2-200 pmol P450/electrode were adsorbed on the surface of nanostructured electrochemical interfaces. Electron transfer, direct electrochemical reduction and interaction with P450 substrates (oxygen, benzphetamine, and lanosterol) and with P450 inhibitor (ketoconazole) were analyzed using cyclic voltammetry (CV), square wave voltammetry (SWV) differential pulse voltammetry (DPV), and amperometry.
Shumyantseva
V V
VV
Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Pogodinskaya Street, 10, Moscow 119121, Russia. viktoria.shumyantseva@ibmc.msk.ru
Bulko
T V
TV
Rudakov
Yu O
YO
Kuznetsova
G P
GP
Samenkova
N F
NF
Lisitsa
A V
AV
Karuzina
I I
II
Archakov
A I
AI
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000737:chemistry; D001589:Benzphetamine / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D002384:Catalysis; D019388:Cytochrome P-450 CYP1A2 / Q000737:chemistry; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry*; D000072467:Cytochrome P450 Family 2; D006046:Gold; D007654:Ketoconazole / Q000737:chemistry; D007810:Lanosterol / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry; D011199:Potentiometry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D011817:Rabbits; D058886:Sterol 14-Demethylase
D000644:Quaternary Ammonium Compounds; D001589:Benzphetamine; C046112:didodecyldimethylammonium; D007810:Lanosterol; D006046:Gold; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D001189:Aryl Hydrocarbon Hydroxylases; D019388:Cytochrome P-450 CYP1A2; D000072467:Cytochrome P450 Family 2; C465241:cytochrome P-450 CYP2B4 (rabbit); D058886:Sterol 14-Demethylase; D007654:Ketoconazole