The humoral pattern recognition receptor PTX3 is stored in neutrophil granules and localizes in extracellular traps.
The long pentraxin (PTX) 3 is produced by macrophages and myeloid dendritic cells in response to Toll-like receptor agonists and represents a nonredundant component of humoral innate immunity against selected pathogens. We report that, unexpectedly, PTX3 is stored in specific granules and undergoes release in response to microbial recognition and inflammatory signals. Released PTX3 can partially localize in neutrophil extracellular traps formed by extruded DNA. Eosinophils and basophils do not contain preformed PTX3. PTX3-deficient neutrophils have defective microbial recognition and phagocytosis, and PTX3 is nonredundant for neutrophil-mediated resistance against Aspergillus fumigatus. Thus, neutrophils serve as a reservoir, ready for rapid release, of the long PTX3, a key component of humoral innate immunity with opsonic activity.
Jaillon
Sébastien
S
Institut National de la Santé et de la Recherche Médicale, Equipe Avenir, Unité 564, University Hospital of Angers, University of Angers, Angers 49933, France.
Peri
Giuseppe
G
Delneste
Yves
Y
Frémaux
Isabelle
I
Doni
Andrea
A
Moalli
Federica
F
Garlanda
Cecilia
C
Romani
Luigina
L
Gascan
Hugues
H
Bellocchio
Silvia
S
Bozza
Silvia
S
Cassatella
Marco A
MA
Jeannin
Pascale
P
Mantovani
Alberto
A
D000818:Animals; D002097:C-Reactive Protein / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D003594:Cytoplasmic Granules / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism*; D005786:Gene Expression Regulation; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009504:Neutrophils / Q000166:cytology* / Q000378:metabolism; D011485:Protein Binding; D000683:Serum Amyloid P-Component / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D000099153:Pentraxins
D002097:C-Reactive Protein; D000683:Serum Amyloid P-Component; D000099153:Pentraxins