title: Mast cells can modulate leukocyte accumulation and skeletal muscle function following hindlimb unloading.
abstract: Rodent hindlimb suspension is widely used to induce inflammation and muscle impairment. We set out to define the role of mast cells in neutrophil and macrophage recruitment and muscle recovery after unloading-reloading. We hypothesized that mechanical perturbation would stimulate release of proinflammatory substances by mast cells, which would influence leukocyte recruitment and muscle function. Rats were suspended for 10 days and injected with a mast cell inhibitor (cromolyn) or stimulator (compound 48/80) or a placebo before reloading. Leukocyte accumulation and muscle function were assessed using immunohistological staining and measurements of contractile properties in vitro. Our results showed that mechanical loading activated mast cells, thereby influencing leukocyte recruitment in the early reloading periods. Indeed, the inhibition of mast cell degranulation significantly reduced the number of neutrophil cell profiles in reloaded soleus muscle, whereas mast cell activation provoked a significant increase in the number of neutrophil cell profiles in uninjured muscle. However, the inhibition of mast cell degranulation also led to a significant increase in the number of ED1+ macrophage cell profiles. These perturbations in the inflammatory response caused by mast cell inhibition induced a short protective effect on the loss of muscle force after 1 day of reloading but delayed the return to the normal contractile properties of muscles after 14 days of reloading. These results indicate that mechanical loading can induce mast cell degranulation, which can influence leukocyte influx and muscle function, and also highlighted the possibility that leukocytes may play a dual role in skeletal muscles.
authors: Centre Hospitalier Universitaire de Québec-Centre de Recherche du Centre Hospitalier de l'Université Laval, Canada.
authors: Nicolas
authors: N
authors: Dumont
authors: Katherine
authors: K
authors: Lepage
authors: Claude H
authors: CH
authors: Côté
authors: Jérôme
authors: J
authors: Frenette
mesh_terms: D000818:Animals; D019898:Autocrine Communication; D015550:Cell Degranulation / Q000187:drug effects; D004205:Cromolyn Sodium / Q000494:pharmacology; D004195:Disease Models, Animal; D005260:Female; D019417:Hindlimb Suspension; D007249:Inflammation / Q000473:pathology / Q000503:physiopathology*; D008264:Macrophages / Q000473:pathology; D008407:Mast Cells / Q000187:drug effects / Q000473:pathology*; D009119:Muscle Contraction; D018482:Muscle, Skeletal / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology*; D009135:Muscular Diseases / Q000473:pathology / Q000503:physiopathology*; D020556:Neutrophil Infiltration / Q000187:drug effects; D009504:Neutrophils / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function; D013314:Stress, Mechanical; D013997:Time Factors; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
chemical_list: D003189:p-Methoxy-N-methylphenethylamine; D004205:Cromolyn Sodium