title: Selective and non-selective autophagic degradation of mitochondria in yeast.
abstract: Mitochondria are essential to oxidative energy production in aerobic eukaryotic cells, where they are also required for multiple biosynthetic pathways to take place. Mitochondrial homeostasis also plays a crucial role in ageing and programmed cell death, and recent data have suggested that mitochondria degradation is a strictly regulated process. Autophagy is an evolutionary conserved mechanism that provides cells with a mechanism for the continuous turnover of damaged and obsolete macromolecules and organelles. In this work, we investigated mitochondria degradation by autophagy. Electron microscopy observations of yeast cells submitted to nitrogen starvation after growth on different carbon sources provided evidence that microautophagy, rather than macroautophagy, preferentially occurred in cells grown under nonfermentable conditions. The observation of mitochondria degradation showed that both a selective process and a nonselective process of mitochondria autophagy occurred successively. In a yeast strain inactivated for the gene UTH1, the selective process was not observed.
authors: CNRS, UMR 5095, Bordeaux, France.
authors: Ingrid
authors: I
authors: Kissová
authors: Bénédicte
authors: B
authors: Salin
authors: Jacques
authors: J
authors: Schaeffer
authors: Sapan
authors: S
authors: Bhatia
authors: Stéphen
authors: S
authors: Manon
authors: Nadine
authors: N
authors: Camougrand
mesh_terms: D001343:Autophagy / Q000502:physiology*; D005508:Food Deprivation; D017353:Gene Deletion; D020868:Gene Silencing; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008928:Mitochondria / Q000502:physiology* / Q000648:ultrastructure; D008957:Models, Genetic; D009584:Nitrogen / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics* / Q000378:metabolism; D013997:Time Factors
chemical_list: D049452:Green Fluorescent Proteins; D009584:Nitrogen