title: Effect of the destruction of cells containing the serotonin reuptake transporter on urethrogenital reflexes.
abstract: BACKGROUND
  The urethrogenital (UG) reflex is an autonomic and somatic response that mimics some of the physiological changes seen during ejaculation. The UG reflex is tonically inhibited by neurons in the ventral medulla, an area containing serotonin neurons. AIM; To examine the effect of lesions of brain neurons containing the serotonin reuptake transporter (SERT) on ejaculatory-like reflexes.
  
  
  METHODS
  Anti-SERT saporin (80 nL, 1 mM) or saline was injected bilaterally into the ventrolateral medulla of male Sprague-Dawley rats. Ten to 18 days later, animals were deeply anesthetized and the presence of the UG reflex was examined before and after acute spinal cord transection (T9-10). Following the experiment the presence and number of serotonin and norepinephrine containing neurons (using tryptophan hydroxylase and dopamine beta-hydroxylase, respectively) was performed.
  
  
  METHODS
  The UG reflex and cell counts.
  
  
  RESULTS
  In saline-injected controls the UG reflex was not evoked in the anesthetized, intact preparation, indicating the presence of the supraspinal inhibition, as previously reported. Injection of anti-SERT saporin into the ventrolateral medulla allowed the UG reflex to be activated in the intact preparation, thus removed the inhibition. This was associated with a decrease in the number of serotonin neurons in the ventrolateral medulla and raphe. No change in the number of noradrenergic neurons was observed.
  
  
  CONCLUSIONS
  These studies suggest that ventral medullary neurons containing SERT are involved in the tonic inhibition of the UG reflex.
authors: Division of Urology, Department of Surgery, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
authors: Karla
authors: K
authors: Gravitt
authors: Lesley
authors: L
authors: Marson
mesh_terms: D000818:Animals; D008297:Male; D008526:Medulla Oblongata / Q000378:metabolism* / Q000473:pathology; D009434:Neural Pathways / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012018:Reflex; D012701:Serotonin / Q000378:metabolism*; D050486:Serotonin Plasma Membrane Transport Proteins / Q000378:metabolism*; D012965:Sodium Chloride; D014521:Urethra / Q000294:innervation*
chemical_list: D050486:Serotonin Plasma Membrane Transport Proteins; D012701:Serotonin; D012965:Sodium Chloride