title: Formation of an enolate intermediate is required for the reaction catalyzed by 3-hydroxyacyl-CoA dehydrogenase.
abstract: Fluorinated substrate analogs were synthesized and incubated with rat liver 3-hydroxyacyl-CoA dehydrogenase, which reveals that the formation of an enolate intermediate is required for the reaction catalyzed by the enzyme.
authors: Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, PR China.
authors: Xiaojun
authors: X
authors: Liu
authors: Guisheng
authors: G
authors: Deng
authors: Xiusheng
authors: X
authors: Chu
authors: Nan
authors: N
authors: Li
authors: Long
authors: L
authors: Wu
authors: Ding
authors: D
authors: Li
mesh_terms: D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000737:chemistry* / Q000378:metabolism*; D000214:Acyl Coenzyme A / Q000737:chemistry*; D000818:Animals; D002384:Catalysis; D003065:Coenzyme A / Q000138:chemical synthesis / Q000737:chemistry*; D051381:Rats; D013379:Substrate Specificity
chemical_list: D000214:Acyl Coenzyme A; C010667:acetoacetyl CoA; D015094:3-Hydroxyacyl CoA Dehydrogenases; D003065:Coenzyme A