Online medium-throughput respirometry-based OTR measurements in magnetically stirred cultures.
Intensified bioprocess development requires parallelized medium- to high-throughput experimentation with high on- and offline data density across all early scales of the development trajectory from microtiter plate via shake flask to lab-scale reactor. We developed a widespread measurement principle for intermediate scales, respirometry, into a parallelized oxygen transfer rate measurement device that could accurately record common process development-relevant effects such as acetate formation, diauxic growth, and nutrient limitations. The device was further equipped with dissolved oxygen measurement capability and sampling ports that allowed repetitive monoseptic sample withdrawal without disturbing the cultivation. Optimization of the operating parameters lead to k(L) a values of up to 160 h(-1) and corresponding oxygen transfer rates of 1 g L(-1) h(-1) for cultivation volumes of 50 mL.
Brethauer
Simone
S
ETH Zurich, Bioprocess Laboratory, Institute of Process Engineering, Universitaetsstrasse 6, 8092 Zurich, Switzerland.
Held
Martin
M
Panke
Sven
S
D019149:Bioreactors / Q000382:microbiology; D015374:Biosensing Techniques; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D007218:Industrial Microbiology / Q000295:instrumentation*; D007700:Kinetics; D009584:Nitrogen / Q000378:metabolism; D010100:Oxygen / Q000032:analysis* / Q000378:metabolism*
D009584:Nitrogen; D010100:Oxygen