title: Safrole oxide is a useful tool for investigating the effect of apoptosis in vascular endothelial cells on neural stem cell survival and differentiation in vitro.
abstract: Previously, we found safrole oxide could promote VEC apoptosis, however, it is not known whether it can induce NSC apoptosis. It is reported that neural stem cells (NSCs) are localized in a vascular niche. But the effects of apoptosis in vascular endothelial cells (VEC) on NSC growth and differentiation are not clear. To answer these questions, in this study, we co-cultured NSCs with VECs in order to imitate the situation in vivo, in which NSCs are associated with the endothelium, and treated the single-cultured NSCs and the co-cultured NSCs with safrole oxide. The results showed that safrole oxide (10-100 microg/mL) had no effects on NSC growth. Based on these results, we treated the co-culture system with this small molecule. The results showed that the NSCs differentiation, into neurons and gliacytes was induced by VECs untreated with safrole oxide. But in the co-culture system treated with safrole oxide, the NSCs underwent apoptosis. The data suggested that when VEC apoptosis occurred in the co-culture system, the NSC survival and differentiation could not be maintained, and NSCs died by apoptosis. Our finding provided a useful tool for investigating the effect of apoptosis in vascular endothelial cells on neural stem cell survival and differentiation in vitro.
authors: Institute of Developmental Biology, School of Life Science, Shandong University, Jinan 250100, China.
authors: Le
authors: L
authors: Su
authors: BaoXiang
authors: B
authors: Zhao
authors: Xin
authors: X
authors: Lv
authors: Jing
authors: J
authors: Zhao
authors: ShangLi
authors: S
authors: Zhang
authors: JunYing
authors: J
authors: Miao
mesh_terms: D000818:Animals; D017209:Apoptosis; D002450:Cell Communication; D002454:Cell Differentiation; D002470:Cell Survival; D018920:Coculture Techniques; D053595:Embryonic Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D009474:Neurons / Q000166:cytology* / Q000502:physiology; D012451:Safrole / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: C009196:safrole oxide; D012451:Safrole