HDM2-binding partners: interaction with translation elongation factor EF1alpha.
To understand the cellular functions of HDM2, we attempted to identify novel HDM2-interacting proteins by proteomic analysis. Along with previously identified interactions with the ribosomal proteins, our analysis reveals interactions of HDM2 with the ribosomal translation elongation factor EF1alpha, 40S ribosomal protein S20, tubulins, glyceraldehyde 3-phosphate dehydrogenase, and a proteolysis-inducing factor dermicidin in the absence of tumor suppressor p53. Because a CTCL tumor antigen HD-CL-08 has high degree of homology with EF1alpha, we confirmed interaction of HDM2 with EF1alpha by immunoprecipitation and Western blot analysis in transformed as well as near normal diploid cells. Endogenous HDM2- EF1alpha complex was detected in cancer cells overexpressing HDM2, suggesting a possible role of this interaction in HDM2-mediated oncogenesis. Consistent with their interaction, colocalization of HDM2 and EF1alpha can be detected in the cytoplasm of normal or transformed cells. Amino acid residues 1-58 and 221-325 of HDM2 were found to be essential for its interaction with EF1alpha, suggesting that the interaction is independent of its other ribosomal interacting proteins L5, L11, and L23. Overexpression of HDM2 did not affect translation. Because EF1alpha has been implicated in DNA replication and severing of microtubules, interaction of HDM2 with EF1alpha may signify a p53-independent cell growth regulatory role of HDM2.
Frum
Rebecca
R
Department of Biochemistry and the Massey Cancer Center, Virginia Commonwealth University, Richmond, Virginia 23298, USA.
Busby
Scott A
SA
Ramamoorthy
Mahesh
M
Deb
Sumitra
S
Shabanowitz
Jeffrey
J
Hunt
Donald F
DF
Deb
Swati P
SP
D015153:Blotting, Western; D003593:Cytoplasm / Q000737:chemistry; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation; D009369:Neoplasms / Q000737:chemistry / Q000378:metabolism; D020648:Peptide Elongation Factor 1 / Q000032:analysis / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000235:genetics; D025941:Protein Interaction Mapping; D040901:Proteomics; D051736:Proto-Oncogene Proteins c-mdm2 / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism; D014407:Tumor Cells, Cultured
D020648:Peptide Elongation Factor 1; D012269:Ribosomal Proteins; D014404:Tubulin; C029611:ribosomal protein S20; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2