Use of statistical methods to find the polysaccharide structural characteristics and the relationships between monosaccharide composition ratio and macrophage stimulatory activity of regionally different strains of Lentinula edodes.
Multiple linear regression analysis was used to deduce the correlation between the monosaccharide composition ratios of 10 regionally different strains of Lentinula edodes and their in vitro macrophage stimulatory activities. Arabinose, xylose, mannose and galactose were identified as the monosaccharides that could be related to macrophage stimulatory activities. Additional principal component analysis and factor analysis methods were used to treat the same monosaccharide composition ratio data and the compositions of arabinose, xylose, mannose and galactose were found to be important. Interestingly, glucose, although presented in large compositions in all strains presumably forms the backbone of the polysaccharide structures, is not selected as the determinant factor for either structural characteristics or that of the in vitro macrophage stimulatory activities.
Lo
Tiffany Chien Ting
TC
Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan, ROC.
Jiang
Yi Huei
YH
Chao
Anne Lien Ju
AL
Chang
Cheng Allen
CA
D000818:Animals; D002460:Cell Line; D008264:Macrophages / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009005:Monosaccharides / Q000032:analysis* / Q000494:pharmacology; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D012044:Regression Analysis; D020942:Shiitake Mushrooms / Q000737:chemistry* / Q000145:classification
D009005:Monosaccharides; D011134:Polysaccharides