TRB3 inhibits the transcriptional activation of stress-regulated genes by a negative feedback on the ATF4 pathway.
The integrated stress response (ISR) is defined as a highly conserved response to several stresses that converge to the induction of the activating transcription factor 4 (ATF4). Because an uncontrolled response may have deleterious effects, cells have elaborated several negative feedback loops that attenuate the ISR. In the present study, we describe how induction of the human homolog of Drosophila tribbles (TRB3) attenuates the ISR by a negative feedback mechanism. To investigate the role of TRB3 in the control of the ISR, we used the regulation of gene expression by amino acid limitation as a model. The enhanced production of ATF4 upon amino acid starvation results in the induction of a large number of target genes like CHOP (CAAT/enhancer-binding protein-homologous protein), asparagine synthetase (ASNS), or TRB3. We demonstrate that TRB3 overexpression inhibits the transcriptional induction of CHOP and ASNS whereas TRB3 silencing induces the expression of these genes both under normal and stressed conditions. In addition, transcriptional profiling experiments show that TRB3 affects the expression of many ISR-regulated genes. Our results also suggest that TRB3 and ATF4 belong to the same protein complex bound to the sequence involved in the ATF4-dependent regulation of gene expression by amino acid limitation. Collectively, our data identify TRB3 as a negative feedback regulator of the ATF4-dependent transcription and participates to the fine regulation of the ISR.
Jousse
Céline
C
UMR 1019, Unité Nutrition Humaine, INRA de Theix, 63122 St Genès Champanelle, France.
Deval
Christiane
C
Maurin
Anne-Catherine
AC
Parry
Laurent
L
Chérasse
Yoan
Y
Chaveroux
Cédric
C
Lefloch
Renaud
R
Lenormand
Philippe
P
Bruhat
Alain
A
Fafournoux
Pierre
P
D051701:Activating Transcription Factor 4 / Q000096:biosynthesis* / Q000235:genetics; D000596:Amino Acids / Q000172:deficiency*; D000818:Animals; D001217:Aspartate-Ammonia Ligase / Q000096:biosynthesis / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D020868:Gene Silencing; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008954:Models, Biological; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism* / Q000037:antagonists & inhibitors; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological / Q000235:genetics / Q000378:metabolism; D051742:Transcription Factor CHOP / Q000096:biosynthesis / Q000235:genetics
C497109:ATF4 protein, human; D000596:Amino Acids; C081452:Atf4 protein, mouse; D018797:Cell Cycle Proteins; C497249:DDIT3 protein, human; D012097:Repressor Proteins; C474830:TRB3 protein, mouse; C474831:TRIB3 protein, human; D051701:Activating Transcription Factor 4; D051742:Transcription Factor CHOP; D017346:Protein Serine-Threonine Kinases; D001217:Aspartate-Ammonia Ligase