title: Enhanced synchronous spectrofluorimetric determination of aflatoxin B1 in pistachio samples using multivariate analysis.
abstract: The determination of aflatoxin B(1) (AFB1) in pistachio has been accomplished by normal and synchronous fluorimetry in combination with some multivariate calibration methods and derivative techniques. Extending the two-dimensional synchronous fluorescence scan to a three-dimensional total synchronous fluorescence scan was used to obtain the optimized Deltalambda for AFB1 in pistachio sample. The methods are based on the enhanced fluorescence of AFB1 by beta-cyclodextrine in 10% (v/v) methanol-water solution. Twenty-six pistachio samples containing AFB1 in the range 0-15 ppb were used as calibration set. Eighteen combinational methods were tested to make best model for prediction of AFB1 and finally best results obtained using a method based on synchronous fluorimetry in combination with multiple linear regressions (MLR). For concentrations ranging from 0 to 15 ppb of AFB1 in 22 pistachio samples as prediction set, the values of root mean square difference (RMSD) and relative error of prediction (REP) using MLR were 0.328 and 4.453%, respectively were observed. Two naturally contaminated pistachio samples were analyzed by synchronous fluorimetry using MLR and compared with HPLC results.
authors: Department of Chemistry, Faculty of Science, Tarbiat Modarres University, Tehran, Iran.
authors: Mohammad
authors: M
authors: Aghamohammadi
authors: Javad
authors: J
authors: Hashemi
authors: Gholamreza Asadi
authors: GA
authors: Kram
authors: Naader
authors: N
authors: Alizadeh
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D015999:Multivariate Analysis; D009754:Nuts / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D016604:Aflatoxin B1