Anti-hypercalcemic effect of orally administered recombinant Saccharomyces cerevisiae expressing salmon calcitonin on hypercalcemic rats.
Oral delivery of salmon calcitonin (sCT) to rats via a recombinant Saccharomyces cerevisiae was assessed. A synthetic sCT gene was cloned and expressed in S. cerevisiae yAGA2-sCT. Recombinant salmon calcitonin (rsCT) expression was detected by flow cytometry. The resorption activity of osteoclasts was inhibited by 3 x 10(-6 )M rsCT. Oral administration of 5 g lyophilized yAGA2-sCT/kg to hypercalcemic rats decreased serum calcium from 2.8 +/- 0.02-2.7 +/- 0.02 mM.
Sun
Pingnan
P
College of Marine Life Sciences, Ocean University of China, Qingdao, PR China.
Zhang
Xuecheng
X
Zang
Xiaonan
X
Zhou
Xiaoling
X
Chen
Yunsong
Y
Arunakumara
K K I U
KK
Liang
Bin
B
D000284:Administration, Oral; D000818:Animals; D001862:Bone Resorption / Q000188:drug therapy; D002116:Calcitonin / Q000008:administration & dosage* / Q000627:therapeutic use*; D002118:Calcium / Q000097:blood; D002417:Cattle; D005434:Flow Cytometry; D015870:Gene Expression; D005822:Genetic Vectors; D006934:Hypercalcemia / Q000188:drug therapy*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000627:therapeutic use; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics*
D011994:Recombinant Proteins; C028815:salmon calcitonin; D002116:Calcitonin; D002118:Calcium