Analysis of mouse skin reveals proteins that are altered in a diet-induced diabetic state: a new method for detection of type 2 diabetes.
In this study, proteomic analysis was performed on the skin of C57BL/6J mice with type 2 diabetes and compared to nondiabetic controls. To induce obesity and subsequent diabetes, mice were placed on a high-fat diet for 16 wk. After 16 wk, both diabetic and nondiabetic control mice were sacrificed and their skin removed for analysis. Following 2-DE, proteomic profiles from the skin samples were quantified using PDQuest software. Out of more than 1000 distinct protein spots, 28 were shown to be significantly altered with 6 being decreased and 22 increased in the diabetic state compared to controls. The 28 protein spots were removed from the gels and analyzed by MALDI-TOF and MS/MS analyses. Protein identifications revealed that 17 of the 28 proteins were involved in energy metabolism (60.7% of changes observed). Collectively, none of the significantly altered proteins had been shown previously to be altered in diabetic skin. This study not only helps to identify proteins found in skin samples of obese mice with type 2 diabetes, but also shows that skin biopsies coupled with proteomic analysis may be useful as a noninvasive method for the diagnosis of hyperinsulinemia and diabetes.
List
Edward O
EO
Edison Biotechnology Institute, Ohio University, Athens, OH 45701, USA. edlist@yahoo.com
Berryman
Darlene E
DE
Palmer
Amanda J
AJ
Qiu
Linghua
L
Sankaran
Sudha
S
Kohn
Doug T
DT
Kelder
Bruce
B
Okada
Shigeru
S
Kopchick
John J
JJ
D000818:Animals; D003924:Diabetes Mellitus, Type 2 / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D004041:Dietary Fats / Q000008:administration & dosage; D004734:Energy Metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D011506:Proteins / Q000032:analysis*; D040901:Proteomics; D012867:Skin / Q000737:chemistry* / Q000473:pathology; D015430:Weight Gain
D004041:Dietary Fats; D011506:Proteins