title: Abdominal wall healing in reoperated rats.
abstract: OBJECTIVE
  Experimental evaluation of the abdominal wall healing in reoperations on the same surgical site, by means of macroscopic analysis, histological and breaking strength studies of the surgical scar.
  
  
  METHODS
  Twenty-four rats were selected and divided in 3 groups (G1, G2 and G3). A medium longitudinal laparotomy was performed, followed by laparorrhaphy on two synthesis planes, using an interrupted suture technique. The reoperations were performed in the same way, on the same surgical site. On the first day of the study all the 24 rats were operated, on the 30th day the 16 animals belonging to G2 and G3 were reoperated, and finally, on the 60th day, the 8 rats from G3 were operated for the third and last time. After 30 days of the last laparotomy of each group, euthanasia was performed. Complications such as adhesions were evaluated during the resection of surgical site. The breaking strength study was performed next, followed by the microscopical collagen analysis, using for that histological cuts stained with picrosirius.
  
  
  RESULTS
  The adhesions were prevalent in G2 and G3 (16% each) when compared to G1. No significant difference was found in the breaking strength study. Statistically significant difference was observed in collagen concentration analysis. It was found higher mature collagen (type I) as well as total collagen concentration in the groups operated more than once (G2 and G3). The highest concentration of mature collagen (p<0,0001) and total collagen (p<0,0021) were found in G3 followed by G2 and G1 (in this sequence).
  
  
  CONCLUSIONS
  The highest mature collagen concentration on the experimental groups, points out the importance of inflammatory activity in the healing process; in the resutures of the abdominal wall the surgical scar maturity is faster acquired than in the primary sutures; in relation to the primary sutures, the resutures of the abdominal wall did not influence in the resistance of the surgical scar.
authors: PUCPR, Brazil.
authors: Zacarias Alves de
authors: ZA
authors: Souza Filho
authors: Fernando Hintz
authors: FH
authors: Greca
authors: Lúcia de
authors: Ld
authors: Noronha
authors: André Souza de Albuquerque
authors: AS
authors: Maranhão
authors: Ana Paula
authors: AP
authors: Calil
authors: Danila Pinheiro
authors: DP
authors: Hubie
authors: Fabiana Matos
authors: FM
authors: Barbosa
mesh_terms: D034861:Abdominal Wall / Q000601:surgery*; D000818:Animals; D002921:Cicatrix / Q000473:pathology; D003094:Collagen / Q000032:analysis*; D004195:Disease Models, Animal; D008297:Male; D010537:Peritoneum / Q000601:surgery; D011183:Postoperative Complications / Q000209:etiology; D051381:Rats; D017208:Rats, Wistar; D012086:Reoperation; D013526:Surgical Mesh; D013537:Sutures; D013718:Tensile Strength; D014945:Wound Healing / Q000502:physiology*
chemical_list: D003094:Collagen