[Role of T cells in the pathogenesis of type I diabetes mellitus: structure analysis of complementarity determining region 3 of circulating T cells].
Type 1 diabetes mellitus (type 1 DM) is the disease of insulin deficiency due to the destruction of islet cells of the pancreas, presumably through the pathogenic process mediated by autoreactive T cells. In many autoimmune diseases, oligoclonal expansion of autoreactive T cells have been reported recently. It is also suggested that proliferation of T cell clones which recognize pancreatic beta cell antigen are involved in the pathogenesis of type 1 DM. In this study, the diversity of T cell receptor (TCR) structures were evaluated in patients with type 1 DM by analyzing TCR Vbeta repertoire and complementarity determining region 3 (CDR3) size distributions of circulating T cells. Increase of specific TCR Vbeta repertoires was often observed in patients with positive anti-glutamic acid decarboxylase antibody, and this tendency was more evident among CD8+ T cells than in CD4+ T cells. Reductions of CDR3 sizes were frequently seen among CD8+ T cells from patients whose onset was within 10 years. These results suggested that selective expansion of CD8+ T cell clones play roles in the pathogenesis of type 1 DM.
Sakumai
Masahiro
M
Department of Clinical Laboratory, Kanazawa Medical University Hospital, Kahoku-gun, Ishikawa 920-0293.
Yachie
Akihiro
A
Kasahara
Yoshihito
Y
D000293:Adolescent; D000328:Adult; D001323:Autoantibodies / Q000276:immunology; D001324:Autoantigens / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D022801:Complementarity Determining Regions / Q000276:immunology*; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D005260:Female; D005968:Glutamate Decarboxylase / Q000276:immunology; D006801:Humans; D050417:Insulin-Secreting Cells / Q000276:immunology; D008297:Male; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics / Q000276:immunology
D001323:Autoantibodies; D001324:Autoantigens; D022801:Complementarity Determining Regions; D016693:Receptors, Antigen, T-Cell, alpha-beta; D005968:Glutamate Decarboxylase