Using an indicator displacement assay to monitor glucose oxidase activity in blood serum.
[diagram: see text] Using a boronic acid receptor that was previously found to have high affinity for gluconic acid, we created a colorimetric indicator displacement assay (IDA) that can report the concentration of the product of glucose oxidase (GOx) catalyzed glucose oxidation. The color change obtained directly reflects the concentration of glucose. Our sensing ensemble was then successfully applied to determine the glucose concentration in human serum, which offers a facile, colorimetric, sensitive, and accurate glucose test.
Zhang
Tianzhi
T
Department of Chemistry and Biochemistry, 1 University Station A5300, The University of Texas, Austin, Texas 78712, USA.
Anslyn
Eric V
EV
D001786:Blood Glucose / Q000032:analysis / Q000378:metabolism; D002384:Catalysis; D003124:Colorimetry / Q000379:methods*; D005942:Gluconates / Q000737:chemistry / Q000378:metabolism; D005949:Glucose Oxidase / Q000097:blood* / Q000378:metabolism*; D006801:Humans; D015394:Molecular Structure; D010084:Oxidation-Reduction; D017186:Titrimetry
D001786:Blood Glucose; D005942:Gluconates; D005949:Glucose Oxidase; C030691:gluconic acid