title: Evaluation of antioxidant activity and preventing DNA damage effect of pomegranate extracts by chemiluminescence method.
abstract: The antioxidant activities of three parts (peel, juice, and seed) and extracts of three pomegranate varieties in China were investigated by using a chemiluminescence (CL) method in vitro. The scavenging ability of pomegranate extracts (PEs) on superoxide anion, hydroxide radical, and hydrogen peroxide was determined by the pyrogallol-luminol system, the CuSO4-Phen-Vc-H2O2 system, and the luminol-H2O2 system, respectively. DNA damage preventing the effect of PE was determined by the CuSO4-Phen-Vc-H2O2-DNA CL system. The results showed that the peel extract of red pomegranate had the best effect on the scavenging ability of superoxide anion because its IC50 value (4.01 +/- 0.09 microg/mL) was the lowest in all PEs. The seed extract of white pomegranate could scavenge hydroxide radical most effectively of the nine extracts (the IC50 value was 1.69 +/- 0.03 microg/mL). The peel extract of white pomegranate had the best scavenging ability on hydrogen peroxide, which had the lowest IC50 value (0.032 +/- 0.003 microg/mL) in the nine extracts. The seed extract of white pomegranate (the IC50 value was 3.67 +/- 0.03 microg/mL) was the most powerful on the DNA damage-preventing effect in all of the PEs. Also, the statistical analysis indicated that there were significant differences (at P < 0.05) among the extracts of the different varieties and parts in each system.
authors: Department of Food Chemistry and Molecular Biology, College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors: Shanshan
authors: S
authors: Guo
authors: Qianchun
authors: Q
authors: Deng
authors: Junsong
authors: J
authors: Xiao
authors: Bijun
authors: B
authors: Xie
authors: Zhida
authors: Z
authors: Sun
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects*; D016166:Free Radical Scavengers / Q000737:chemistry; D005638:Fruit / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D017665:Hydroxyl Radical / Q000737:chemistry; D049449:Luminescence; D029561:Lythraceae / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry; D013481:Superoxides / Q000737:chemistry
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D010936:Plant Extracts; D013481:Superoxides; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide