A small chimeric promoter for high prostate-specific transgene expression from adenoviral vectors.
BACKGROUND
Specificity of transgene expression is important for safety during gene therapeutical applications. For prostate cancer, transcriptional targeting has been applied but was hampered by loss of specificity and low activity. We constructed a small chimeric promoter for high and prostate-specific transgene expression from adenoviral vectors.


METHODS
A chimeric promoter, composed of the prostate-specific antigen (PSA) enhancer and the rat probasin promoter, was cloned into an adenoviral vector and its activity was compared to vectors containing conventional prostate-specific promoters and the constitutive Cytomegalovirus (CMV) promoter in in vitro and in vivo prostate cancer models.


RESULTS
The chimeric PSA-probasin promoter was the most active prostate-specific promoter reaching up to 20% of CMV promoter activity while maintaining prostate-specificity.


CONCLUSIONS
The chimeric PSA-probasin promoter is a small promoter that can be utilized in viral vectors for high prostate-specific transgene expression.
Kraaij
Robert
R
Department of Urology, Erasmus MC, Rotterdam, The Netherlands. r.kraaij@erasmusmc.nl
van der Weel
Laura
L
de Ridder
Corrina M A
CM
van der Korput
Hetty A G M
HA
Zweistra
Joke L M
JL
van Rijswijk
Angélique L C T
AL
Bangma
Chris H
CH
Trapman
Jan
J
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000628:therapy*; D000256:Adenoviridae / Q000235:genetics; D000727:Androgen-Binding Protein / Q000235:genetics; D000818:Animals; D045744:Cell Line, Tumor; D003587:Cytomegalovirus / Q000235:genetics; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008856:Microscopy, Fluorescence; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D017430:Prostate-Specific Antigen / Q000235:genetics; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000628:therapy*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D019076:Transgenes
D000727:Androgen-Binding Protein; D011993:Recombinant Fusion Proteins; C027519:probasin; D017430:Prostate-Specific Antigen