Determination of medroxyprogesterone acetate residues by CE immunoassay with chemiluminescence detection.
A rapid and simple method is developed for the determination of medroxyprogesterone acetate (MPA) by CE immunoassay with chemiluminescence (CL). This method is based on the competitive reactions between horseradish peroxidase (HRP)-labeled MPA (MPA-HRP) and free MPA with anti-MPA antiserum. The influencing factors on the electrophoresis and CL detection were studied completely and the optimal conditions of separation and determination were obtained. The linear range was 2.0-50 nmol/L and the LOD for MPA was 0.9 nmol/L. The present method was applied to the analysis of pork tissues.
Peng
Chi-Fang
CF
School of Food Science and Technology, Southern Yangtze University, WuXi, JiangSu Province, PR China.
Huo
Tie-Ming
TM
Liu
Li-Qiang
LQ
Chu
Xiao-Gang
XG
Xu
Chuan-Lai
CL
D000818:Animals; D019075:Electrophoresis, Capillary / Q000379:methods*; D007118:Immunoassay / Q000379:methods*; D008126:Lod Score; D008163:Luminescent Measurements; D017258:Medroxyprogesterone Acetate / Q000032:analysis*; D012680:Sensitivity and Specificity
D017258:Medroxyprogesterone Acetate