[An investigation of restoration of alveolar cleft with engineered bone].
OBJECTIVE
To investigate restoration of alveolar cleft with engineered bone constructed by sponge collagen protein combined bone mesenchymal stem cells (BMSC).


METHODS
Twelve dogs were divided into 4 groups, the third incisor and alveolar bone with periosteum in bilateral maxilla were removed to form alveolar cleft model. The BMSCs were isolated from dog bone marrow. After being cultured and induced, the BMSCs were seeded in sponge collagen protein and cultured for 48 hours. The composites of BMSCs and collagen were implanted into the defect of alveolar cleft. After 12 weeks' feeding, those dogs were sacrificed. Three-dimensional CT and histological examination were used to observe the progress of bone formation.


RESULTS
The defects healed at 12 weeks after being implant BMSCs-collagen composites, the width of engineered bone is resembled with positive control, but the height is less than positive control.


CONCLUSIONS
The engineered bone can restore the defect of alveolar bone effectively, it can be used clinically to treat alveolar cleft.
Ou
Xin-rong
XR
Department of Oral and Maxillofacial Surgery, Xiangya Hospital, Faculty of Stomatology, Centre South University, Changsha 410078, China.
Jian
Xin-chun
XC
Lin
Ge
G
D000539:Alveolar Process / Q000002:abnormalities*; D000543:Alveoloplasty / Q000379:methods*; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D001861:Bone Regeneration; D018929:Cell Culture Techniques; D002972:Cleft Palate / Q000601:surgery*; D003094:Collagen; D004285:Dogs; D005260:Female; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods*
D003094:Collagen