Effects of gastrodin on amino acids after cerebral ischemia-reperfusion injury in rat striatum.
The aim of this study was to explore the effect of gastrodin on the level of amino acids in the striatum in the rats of cerebral ischemia-reperfusion injury. 30 male SD rats were randomly divided into 3 groups: the group of pseudo-operation (normal control group, NC group), the group of cerebral ischemia-reperfusion injury (CIRI group), and the group of cerebral ischemia-reperfusion injury treated with gastrodin (G group). Cerebral ischemia-reperfusion injury was induced through middle cerebral artery occlusion (MCAO). 10 minutes before the operation, the rats in the G group were injected with gastrodin (50 mg/kg) intraperitoneally once. The rats in the CIRI and NC group were injected with the same volume of 10% propylene glycol normal saline intraperitoneally once. The levels of glutamic acid (Glu), aspartic acid (Asp), gamma-aminobutyric acid (GABA), taurine (Tau) in striatum in the rats of the 3 groups were measured with the method of microdialysis-HPLC techniques. The ratio of Glu to GABA was calculated. The volume of cerebral infarction was quantified. This study showed that gastrodin can decrease the volume of cerebral infarction, ameliorate the cerebral injury in the rats of cerebral ischemia-reperfusion. The mechanisms might be that gastrodin can improve the level of amino acids in striatum.
Bie
Xiaodong
X
Department of Gerontology, The Second Affiliated Hospital, School of Medicine-Zhejiang University, Hangzhou, China 310009.
Chen
Yueqing
Y
Han
Jinn
J
Dai
Haibin
H
Wan
Haitong
H
Zhao
Tongfeng
T
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001592:Benzyl Alcohols / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D003342:Corpus Striatum / Q000378:metabolism*; D005960:Glucosides / Q000494:pharmacology*; D020244:Infarction, Middle Cerebral Artery / Q000473:pathology; D008297:Male; D017551:Microdialysis; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism* / Q000517:prevention & control
D000596:Amino Acids; D001592:Benzyl Alcohols; D005960:Glucosides; C045345:gastrodin