Ribosomal protein S1 is not essential for the trans-translation machinery.
In eubacteria, ribosome stalling during protein synthesis is rescued by a tmRNA-derived trans-translation system. Because ribosomal protein S1 specifically binds to tmRNA with high affinity, it is considered to be involved in the trans-translation system. However, the role of S1 in trans-translation is still unclear. To study the function of S1 in the trans-translation system, we constructed an S1-free cell-free translation system. We found that trans-translation proceeded even in the absence of S1. Addition of S1 into the S1-free system did not affect trans-translation efficiency. These results suggest that S1 does not play a role in the trans-translation machinery.
Qi
Hao
H
The Department of Medical Genome Sciences, Graduate School of Frontier Sciences, the University of Tokyo, FSB-401, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan.
Shimizu
Yoshihiro
Y
Ueda
Takuya
T
D001483:Base Sequence; D002474:Cell-Free System; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D029968:Escherichia coli Proteins / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis / Q000502:physiology*; D012329:RNA, Bacterial / Q000235:genetics; D012097:Repressor Proteins / Q000096:biosynthesis / Q000235:genetics; D012269:Ribosomal Proteins / Q000235:genetics / Q000502:physiology*; D012270:Ribosomes / Q000235:genetics / Q000502:physiology*; D013762:Tetrahydrofolate Dehydrogenase / Q000096:biosynthesis / Q000235:genetics; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics; D054334:Viral Regulatory and Accessory Proteins
D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D012329:RNA, Bacterial; D012097:Repressor Proteins; D012269:Ribosomal Proteins; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C016649:phage repressor proteins; C015494:ribosomal protein S1; C096222:tmRNA; D013762:Tetrahydrofolate Dehydrogenase