DNA cleavage promoted by 2,9-dimethyl-4,7-diazadecane-2,9-dithiol (DDD) derivatives.
Three piperidine derivatives of 2,9-dimethyl-4,7-diazadecane-2,9-dithiol (DDD), NEPDDD, NEMPDDD, and NEMMPDDD, were synthesized and used as catalysts in DNA cleavage. Under physiological conditions, a series of experiments have been done. The effects of DNA cleavage with three ligands were studied under different concentrations, cleavage time, and pH values. The results strongly suggested that the plasmid DNA (pUC 19) can be cleaved efficiently by these ligands. For the cleavage reaction catalyzed by NEMPDDD, Form I DNA could convert to Form II completely, and the DNA-cleavage mechanism involved an oxidative pathway.
Zhao
Yuan-Cong
YC
Department of Chemistry, Key Laboratory of Green Chemistry and Technology (Ministry of Education), Sichuan University, Chengdu 610064, PR China.
Zhang
Ji
J
Huang
Yu
Y
Wang
Guan-Quan
GQ
Yu
Xiao-Qi
XQ
D004247:DNA / Q000187:drug effects* / Q000378:metabolism; D053837:DNA Cleavage / Q000187:drug effects*; D010880:Piperidines / Q000138:chemical synthesis / Q000494:pharmacology*; D010957:Plasmids / Q000235:genetics; D014050:Toluene / Q000031:analogs & derivatives / Q000737:chemistry
D010880:Piperidines; D014050:Toluene; D004247:DNA; C004848:dithiol