Study of the SH3-domain GRB2-like 2 gene expression in laryngeal carcinoma.
BACKGROUND
Laryngeal carcinoma is a common malignant tumor of the upper respiratory tract, and in 95% of cases the tumor is laryngeal squamous cell carcinoma (LSCC). The abnormity of SH3-domain GRB2-like 2 (SH3GL2) gene was found in LSCC. In order to clarify the relationship between SH3GL2 gene and LSCC, we evaluated the expression of the SH3GL2 gene in LSCC.


METHODS
Real-time PCR, immunohistochemistry and Western blotting were used to detect the mRNA and protein expression and find the various rules of SH3GL2 gene in LSCC.


RESULTS
The result of real-time PCR showed that the expression level of SH3GL2 mRNA in LSCC tissue was apparently down-regulated; immunohistochemical analysis showed that SH3GL2 protein was mainly located in cytoplasm, the rate of positive cells and SH3GL2 protein expression level were fluctuated with the pathological classification of LSCC; the result of Western blotting showed that SH3GL2 protein was down-regulated significantly in LSCC samples, especially in metastatic lymph nodes.


CONCLUSIONS
These results suggest that SH3GL2 is a LSCC related gene and its expression level is fluctuated with the pathological classification which indicate that SH3GL2 participates in the development and progression of LSCC. And it may be considered as a novel tumor marker to find both a new anti-oncogene and relative factors of invasion and metastasis of laryngeal carcinoma.
Shang
Chao
C
Department of Medical Genetics, China Medical University, Shenyang 110001, China.
Fu
Wei-neng
WN
Guo
Yan
Y
Huang
Dai-fa
DF
Sun
Kai-lai
KL
D048868:Adaptor Proteins, Signal Transducing / Q000032:analysis / Q000235:genetics*; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D007822:Laryngeal Neoplasms / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018909:src Homology Domains
D048868:Adaptor Proteins, Signal Transducing; C107640:SH3GL2 protein, human