In-source decay causes artifacts in SELDI-TOF MS spectra.
SELDI-TOF MS is a mass spectrometric technique which has been extensively used for biomarker discovery. In this study, we show that in-source decay is an important source for the generation of additional spectral peaks with this technique, both for pure proteins and proteins in serum samples. Thus, SELDI-TOF MS could be used to gain sequence information from proteins, but the results also question the uncritical use of SELDI-TOF MS as a general method for the detection of biomarkers.
Ekblad
Lars
L
Baldetorp
Bo
B
Fernö
Mårten
M
Olsson
Håkan
H
Bratt
Charlotte
C
D000818:Animals; D016477:Artifacts; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000032:analysis; D002417:Cattle; D006801:Humans; D040081:Protein Array Analysis / Q000592:standards*; D040901:Proteomics / Q000379:methods* / Q000592:standards*; D044967:Serum / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000592:standards*
D015415:Biomarkers; D001798:Blood Proteins; D012710:Serum Albumin, Bovine