Characterization of PsTX-60B, a new membrane-attack complex/perforin (MACPF) family toxin, from the venomous sea anemone Phyllodiscus semoni.
The sea anemone Phyllodiscus semoni has been known as one of the most venomous sea anemones. Previously, we reported the isolation of the major lethal protein toxins, PsTX-60A and PsTX-60B, from P. semoni and the primary structure of PsTX-60A. In this paper, we report the complete sequence of cDNA (1600 base pairs) encoding PsTX-60B and the deduced primary structure (488 amino acids) of PsTX-60B. The amino acid sequence of PsTX-60B showed the homology with those of PsTX-60A and Actineria villosa toxin, AvTX-60A. The results showed that PsTX-60B is a new member of the membrane-attack complex/perforin (MACPF) family toxin.
Satoh
Hiroshi
H
Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
Oshiro
Naomasa
N
Iwanaga
Setsuko
S
Namikoshi
Michio
M
Nagai
Hiroshi
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003064:Cnidarian Venoms / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D008969:Molecular Sequence Data; D054353:Perforin / Q000235:genetics*; D012615:Sea Anemones / Q000737:chemistry*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D003064:Cnidarian Venoms; D018076:DNA, Complementary; D054353:Perforin