[Investigation of Streptomyces plasmid pSS27].
A bank of Streptomyces plasmid pSS27 fragments was created on the basis of the vector pBluescript II SK(+) and a bifunctional vector pWHM4. Strains of Escherichia coli DH1 and JM109 were used as recipients of hybrid DNA. Selected transformants contained hybrid molecules of DNA with inserts of Streptomyces plasmid from 0.5 kb up to 12.3 kb. Some thiostrepton-resistant transformants which contained extrachromosomal DNA with the molecular size of 18.1 kb were obtained by means of transformation by hybrid plasmid DNA pSW51 (18.1 kb) of S. levoris 165 protoplasts. The analysis of nucleotide sequences of some hybrid plasmids has shown homology of sequencing parts of cloned fragments of plasmid pSS27 with sequences of fibers (chromosomal genes), which took part in sporulation of S. coelicolor A3 (2) and S. avermitilis MA-4680 and Tra B gene of S. phaeochromogenes plasmid pJV1.
Luk'ianchuk
V V
VV
Polishchuk
L V
LV
Matseliukh
B P
BP
D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D010957:Plasmids; D013302:Streptomyces / Q000235:genetics*
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes