[Quality of Neisseria gonorrhoeae DNA detection by polymerase chain reaction: the Federal External Quality Assessment System data].
An analysis of the data submitted in 2005 by 75 laboratories from 31 Russian Federation's regions, participated in the area of the Federal external quality assessment system (FEQAS) "Polymerase chain reaction detection of Neisseria gonorrhoeae" in 2005 indicated that for identification of Neisseria gonorrhoeae DNA, the FEQAS participants used mainly reagents made by Russian manufacturers; the proportion of correct results obtained in the detection of Neisseria gonorrhoeae DNA in the control samples with a DNA concentrations of 10(4) and 10(5) copies/ml was greater than 90% whereas it decreased to 65-75% when the concentration of DNA was 10' copies/ml; there were no significant differences in the proportion of correct results obtained when two most common sets were used to isolate DNA; the proportion of correct results for control samples with a DNA concentration of 10(3) copies/ml was distinctly higher when hybridization with fluorescence detection was employed as a method for detecting the products of Neisseria gonorrhoeae DNA amplification than electrophoresis was applied.
Tvorogova
M G
MG
Malakhov
V N
VN
D001431:Bacteriological Techniques; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D007753:Laboratories; D009344:Neisseria gonorrhoeae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011786:Quality Control
D004269:DNA, Bacterial