title: Bacterial-binding activity and plasma concentration of ladderlectin in rainbow trout (Oncorhynchus mykiss).
abstract: Soluble, defense lectins bind conserved microbial patterns leading to pathogen opsonization, enhanced phagocytosis and activation of complement. These immune functions, however, vary widely among individuals due to genetic and acquired differences affecting binding capacity or plasma concentration. Most evidence for the defensive function of soluble lectins is based on mammals, but several functionally homologous, but less well-characterized, lectins have been identified in fish. In this study, we compared binding of rainbow trout plasma ladderlectin to relevant, intact bacterial targets. A polyclonal antiserum raised against a synthetic peptide identical to the 20 N-terminal amino acids of the reduced 16 kDa rainbow trout ladderlectin subunit was used to detect plasma ladderlectin in immunoblots and indirect enzyme-linked immunosorbent assay (ELISA). Ladderlectin binding to Aeromonas salmonicida subsp. salmonicida, Aeromonas hydrophila, Yersinia ruckeri and Pseudomonas sp. was detected by PAGE and immunoblots of saccharide elutions from intact bacteria incubated in the presence of normal trout plasma. Although plasma concentrations of immunoreactive ladderlectin were low in the majority of trout, significant (P < 0.0001) variation between individual fish was observed in two separate populations. In addition, one population demonstrated a subset of individuals whose ladderlectin levels were approximately seven-fold higher than the population median. These findings indicate that rainbow trout have variable amounts of plasma ladderlectin capable of binding to the surfaces of several relevant bacterial targets.
authors: Fish Pathology Laboratory, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada, N1G 2W1.
authors: Karrie M
authors: KM
authors: Young
authors: Spencer
authors: S
authors: Russell
authors: Mackenzie
authors: M
authors: Smith
authors: Paul
authors: P
authors: Huber
authors: Vaughn E
authors: VE
authors: Ostland
authors: Andrew S
authors: AS
authors: Brooks
authors: M
authors: M
authors: Anthony Hayes
authors: John S
authors: JS
authors: Lumsden
mesh_terms: D016980:Aeromonas hydrophila / Q000276:immunology; D048409:Aeromonas salmonicida / Q000276:immunology; D000818:Animals; D001424:Bacterial Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D015153:Blotting, Western / Q000662:veterinary; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology*; D029941:Fish Proteins / Q000097:blood / Q000276:immunology*; D037102:Lectins / Q000097:blood / Q000276:immunology*; D017686:Oncorhynchus mykiss / Q000097:blood / Q000276:immunology*; D011549:Pseudomonas / Q000276:immunology; D044124:Yersinia ruckeri / Q000276:immunology
chemical_list: D029941:Fish Proteins; D037102:Lectins; C106303:ladderlectin, Oncorhynchus mykiss