Microfluidic chip accomplishing self-fluid replacement using only capillary force and its bioanalytical application.
A polymer microfluidic chip accomplishing automated sample flow and replacement without external controls and an application of the chip for bioanalytical reaction were described. All the fluidic operations in the chip were achieved by only natural capillary flow in a time-planned sequence. For the control of the capillary flow, the geometry of the channels and chambers in the chip was designed based on theoretical considerations and numerical simulations. The microfluidic chip was made by using polymer replication techniques, which were suitable for fast and cheap fabrication. The test for a biochemical analysis, employing an enzyme (HRP)-catalyzed precipitation reaction, exhibited a good performance using the developed chip. The presented microfluidic method would be applicable to biochemical lab-on-a-chips with integrated fluid replacement steps, such as affinity elution and solution exchange during biosensor signaling.
Chung
Kwang Hyo
KH
Biosensor Team, ETRI, Daejeon 305-350, Republic of Korea.
Hong
Jung Woo
JW
Lee
Dae-Sik
DS
Yoon
Hyun C
HC
D015374:Biosensing Techniques; D003198:Computer Simulation; D004798:Enzymes / Q000737:chemistry; D004867:Equipment Design; D006735:Horseradish Peroxidase / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D008956:Models, Chemical; D015233:Models, Statistical; D008962:Models, Theoretical; D010544:Peroxidases / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011312:Pressure; D013499:Surface Properties; D013997:Time Factors
D004798:Enzymes; D011108:Polymers; D006735:Horseradish Peroxidase; D010544:Peroxidases