Microcirculatory changes in experimental mesenteric longitudinal ulcers of the small intestine in rats.
Rats receiving intracolonic administration of indomethacin develop longitudinal ulcers on the mesenteric side of the small intestine that are similar to those seen in the acute phase of Crohn's disease. To investigate the causative role of microcirculatory disturbances and to elucidate the therapeutic effect of antioxidants on this enteropathy in rats, we serially evaluated changes in regional blood flow of the small intestine using laser Doppler perfusion imaging and the colored microsphere injection method. Both methods disclosed stepwise hyperperfusion limited to the mesenteric side of the small intestine following transient ischemia during the initial 30-60 minutes. In addition, both a radical scavenger and a radical production inhibitor significantly ameliorated the mesenteric longitudinal ulcers. We concluded that ischemia-reperfusion on the mesenteric side accompanying excessive production of radicals might be strongly involved in indomethacin-induced longitudinal ulcers of the small intestine in rats.
Shimizu
Kayoko
K
Division of Gastroenterology, Department of Medicine, Kawasaki Medical School, Kurashiki, Japan. shimi@med.kawasaki-m.ac.jp
Koga
Hideki
H
Iida
Mitsuo
M
Haruma
Ken
K
D000493:Allopurinol / Q000627:therapeutic use*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000633:toxicity; D000983:Antipyrine / Q000031:analogs & derivatives* / Q000627:therapeutic use; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D018450:Disease Progression; D000077553:Edaravone; D004797:Enzyme-Linked Immunosorbent Assay; D016166:Free Radical Scavengers / Q000627:therapeutic use*; D007077:Ileal Diseases / Q000139:chemically induced / Q000503:physiopathology* / Q000517:prevention & control; D007082:Ileum / Q000098:blood supply / Q000187:drug effects / Q000378:metabolism; D007213:Indomethacin / Q000633:toxicity; D017078:Laser-Doppler Flowmetry; D008297:Male; D008643:Mesentery / Q000098:blood supply*; D008833:Microcirculation / Q000187:drug effects / Q000503:physiopathology*; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012039:Regional Blood Flow / Q000187:drug effects / Q000502:physiology; D014456:Ulcer / Q000139:chemically induced / Q000503:physiopathology* / Q000517:prevention & control
D000894:Anti-Inflammatory Agents, Non-Steroidal; D016207:Cytokines; D016166:Free Radical Scavengers; D000493:Allopurinol; D009195:Peroxidase; D000077553:Edaravone; D000983:Antipyrine; D007213:Indomethacin