title: Hsp90 is required to localise cyclin B and Msps/ch-TOG to the mitotic spindle in Drosophila and humans.
abstract: During mitosis, cyclin B is extremely dynamic and although it is concentrated at the centrosomes and spindle microtubules (MTs) in organisms ranging from yeast to humans, the mechanisms that determine its localisation are poorly understood. To understand how cyclin B is targeted to different locations in the cell we have isolated proteins that interact with cyclin B in Drosophila embryo extracts. Here we show that cyclin B interacts with the molecular chaperone Hsp90 and with the MT-associated protein (MAP) Mini spindles (Msps; the Drosophila orthologue of XMAP215/ch-TOG). Both Hsp90 and Msps are concentrated at centrosomes and spindles, and we show that Hsp90, but not Msps, is required for the efficient localisation of cyclin B to these structures. We find that, unlike what happens with other cell cycle proteins, Hsp90 is not required to stabilise cyclin B or Msps during mitosis. Thus, we propose that Hsp90 plays a novel role in regulating the localisation of cyclin B and Msps during mitosis.
authors: The Wellcome Trust/Cancer Research UK Gurdon Institute of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QN, UK. r.basto@gurdon.cam.ac.uk
authors: Renata
authors: R
authors: Basto
authors: Fanni
authors: F
authors: Gergely
authors: Viji M
authors: VM
authors: Draviam
authors: Hiroyuki
authors: H
authors: Ohkura
authors: Kathryn
authors: K
authors: Liley
authors: Jordan W
authors: JW
authors: Raff
mesh_terms: D000818:Animals; D019926:Cyclin B / Q000378:metabolism*; D004330:Drosophila / Q000166:cytology* / Q000196:embryology; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004625:Embryo, Nonmammalian; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D008941:Spindle Apparatus / Q000378:metabolism*
chemical_list: C454180:CKAP5 protein, human; D019926:Cyclin B; D029721:Drosophila Proteins; D018841:HSP90 Heat-Shock Proteins; D008869:Microtubule-Associated Proteins; D011993:Recombinant Fusion Proteins; C121511:msps protein, Drosophila