title: Role of nontoxic components of serotype D botulinum toxin complex in permeation through a Caco-2 cell monolayer, a model for intestinal epithelium.
abstract: Botulinum neurotoxin (BoNT) is produced as a large toxin complex (TC) associated with nontoxic nonhemagglutinin (NTNHA) and three hemagglutinin subcomponents (HA-70, -33 and -17). To assess the role of nontoxic components in the oral intoxication of botulinum TCs, we investigated the permeability of serotype D strain 4947 BoNT and its various TC species through cultured Caco-2 cell monolayers. The L-TC species (complexes composed of BoNT, NTNHA, HA-70, HA-33 and HA-17) showed potent permeability through the cell layer, whereas free BoNT, M-TC (BoNT and NTNHA complexes) and M-TC/HA-70 showed little or no permeability. Cell binding tests demonstrated that HA-33/HA-17 complexes bound to cells, whereas other components did not. These findings suggest that BoNT in the 650-kDa L-TC permeates into the cell mainly in an HA-33/HA-17-mediated manner, although free BoNT can permeate into the cell. As free BoNT and M-TC were susceptible to digestion with gastrointestinal juice, it is likely that L-TC species containing HA-33 caused higher oral toxicity in mice than others. We conclude that the HA-33 subcomponent plays a critical role in the permeation of TCs into intestinal epithelium, and that other HA subcomponents protect BoNT against gastrointestinal digestion.
authors: Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture, Abashiri, Japan. k3niwa@bioindustry.nodai.ac.jp
authors: Koichi
authors: K
authors: Niwa
authors: Kumiko
authors: K
authors: Koyama
authors: Shin-Ichi
authors: S
authors: Inoue
authors: Tomonori
authors: T
authors: Suzuki
authors: Kimiko
authors: K
authors: Hasegawa
authors: Toshihiro
authors: T
authors: Watanabe
authors: Toshihiko
authors: T
authors: Ikeda
authors: Tohru
authors: T
authors: Ohyama
mesh_terms: D000818:Animals; D001905:Botulinum Toxins / Q000378:metabolism* / Q000633:toxicity; D018938:Caco-2 Cells; D005750:Gastric Juice / Q000378:metabolism; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D051379:Mice; D010539:Permeability; D011485:Protein Binding; D021122:Protein Subunits / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D021122:Protein Subunits; C096325:botulinum toxin type D; D001905:Botulinum Toxins