title: Response of cells on surface-induced nanopatterns: fibroblasts and mesenchymal progenitor cells.
abstract: Ultrathin films of a poly(styrene)-block-poly(2-vinylpyrindine) diblock copolymer (PS-b-P2VP) and poly(styrene)-block-poly(4-vinylpyrindine) diblock copolymer (PS-b-P4VP) were used to form surface-induced nanopattern (SINPAT) on mica. Surface interaction controlled microphase separation led to the formation of chemically heterogeneous surface nanopatterns on dry ultrathin films. Two distinct nanopatterned surfaces, namely, wormlike and dotlike patterns, were used to investigate the influence of topography in the nanometer range on cell adhesion, proliferation, and migration. Atomic force microscopy was used to confirm that SINPAT was stable under cell culture conditions. Fibroblasts and mesenchymal progenitor cells were cultured on the nanopatterned surfaces. Phase contrast and confocal laser microscopy showed that fibroblasts and mesenchymal progenitor cells preferred the densely spaced wormlike patterns. Atomic force microscopy showed that the cells remodelled the extracellular matrix differently as they migrate over the two distinctly different nanopatterns.
authors: Max-Planck Institute for Polymer Research, 10 Ackermannweg, 55128 Mainz, Germany. khor@mpip-mainz.mpg.de
authors: Hwei Ling
authors: HL
authors: Khor
authors: Yujun
authors: Y
authors: Kuan
authors: Hildegard
authors: H
authors: Kukula
authors: Kaoru
authors: K
authors: Tamada
authors: Wolfgang
authors: W
authors: Knoll
authors: Martin
authors: M
authors: Moeller
authors: Dietmar W
authors: DW
authors: Hutmacher
mesh_terms: D018929:Cell Culture Techniques; D002465:Cell Movement; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000502:physiology; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry*; D011137:Polystyrenes; D011145:Polyvinyls; D013499:Surface Properties
chemical_list: D011137:Polystyrenes; D011145:Polyvinyls; C521881:poly(styrene)-block-poly(2-vinylpyrindine) diblock copolymer; C521882:poly(styrene)-block-poly(4-vinylpyrindine) diblock copolymer