Haloferax larsenii sp. nov., an extremely halophilic archaeon from a solar saltern.
Three strains of Gram-negative, aerobic, neutrophilic, extremely halophilic archaea, designated ZJ206(T), ZJ203 and ZJ204, were isolated from a solar saltern in Zhe-Jiang Province, China. Phenotypically and on the basis of 16S rRNA gene sequences, the strains were very similar. Comparative 16S rRNA gene analysis revealed 96.4-97.4 % sequence similarity to members of the genus Haloferax. The major polar lipids were C(20)C(20) derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, diglycosyl glycerol diether and sulfated diglycosyl diether. The DNA G+C content of strain ZJ206(T) was 62.2 mol%. The results of DNA-DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of the isolates from closely related species. Therefore the isolates should be classified as members of a novel species, for which the name Haloferax larsenii sp. nov. is proposed. The type strain is ZJ206(T) (=CGMCC 1.5347(T)=JCM 13917(T)).
Xu
Xue-Wei
XW
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.|College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
Wu
Yue-Hong
YH
College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
Wang
Chun-Sheng
CS
Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, People's Republic of China.
Oren
Aharon
A
Institute of Life Sciences and the Moshe Shilo Minerva Centre for Marine Biogeochemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Zhou
Pei-Jin
PJ
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.
Wu
Min
M
College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
D002681:China; D019641:DNA, Archaeal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D019621:Haloferax / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012965:Sodium Chloride; D012988:Soil Microbiology; D014871:Water Microbiology
D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride