[Experimental study of gene transfer of insulin-like growth factor I on wound healing of scald rats].
OBJECTIVE
To probe into the effect of IGF-I gene transfer on wound healing of scald rats.


METHODS
Eukaryotic recombinant plasmid of pcDNA3.1/ IGF-I (successfully constructed by our study group earlier) transported by liposome were injected subdermally in wound region of scald rats. Changes of body weight and tendency of wound healing were observed carefully within 5 weeks postburn. The expression of IGF-I gene transfer in serum and liver was observed with immunohistochemical staining.


RESULTS
The results of animal experiment showed that IGF-I gene transported by liposome expressed positively in fibroblast in burn wound, but expressed negatively in liver tissue with immunohistochemical staining, There were no significant differences for IGF-I concentrations in serum among treatment group and control group (P > 0.05). The speed of wound healing for animals injected with IGF-I reconstruct was faster than those which didn't injected IGF-I (P < 0.05); The body weight of the animals in the group injected with IGF-I hadn't a loss in 5 weeks postburn, but those which didn't injected IGF-I had a loss in body weight during postburn days ( P < 0.05).


CONCLUSIONS
Gene transfer of IGF-I transported by liposome can promote wound healing, and it also could avoid some adverse effect brought by application of IGF-I systematically .
Yang
Lei
L
Department of Burn and Plastic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Gao
Jian-hua
JH
Wang
Jia-han
JH
D000818:Animals; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D007334:Insulin-Like Growth Factor I / Q000235:genetics*; D008081:Liposomes; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014162:Transfection; D014945:Wound Healing / Q000235:genetics*
D008081:Liposomes; D007334:Insulin-Like Growth Factor I