Analysis of the immune-inducible genes of Plutella xylostella using expressed sequence tags and cDNA microarray.
In the present study, the complex gene expression responses of Plutella xylostella to microbial challenges and injury were surveyed using a newly constructed expressed sequence tag (EST) clone collection and cDNA microarray analysis. A total of 1132 P. xylostella ESTs were cloned, annotated and categorized by their putative functions; these included proteases, protease inhibitors, recognition molecules and anti-microbial peptides. GeneOntology revealed that 4% of the P. xylostella ESTs corresponded to immunity-related genes potentially involved in innate immunity. We then used microarray analysis to identify 44 genes that were differentially expressed with at least a two-fold expression difference in P. xylostella before and after pathogen challenge. Together, our EST categorization and microarray profiling analyses allowed us to identify 70 genes that should be considered candidate immune response genes, providing important new insights into the molecular events that occur during the innate immune response in P. xylostella.
Eum
Jai Hoon
JH
Cell Engineering and 3-D Structure Laboratory, School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
Seo
Young Rok
YR
Yoe
Sung Moon
SM
Kang
Seok Woo
SW
Han
Sung Sik
SS
D000818:Animals; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015723:Gene Library; D017344:Genes, Insect; D007113:Immunity, Innate / Q000235:genetics; D019476:Insect Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis
D019476:Insect Proteins