Peptide immobilization on amine-terminated boron-doped diamond surfaces.
This paper reports on the formation and characterization of semicarbazide termination on aminated boron-doped diamond (BDD) surfaces, and further preparation of peptide microarray through site-specific alpha-oxo semicarbazone ligation. Hydrogen-terminated BDD electrodes were first aminated using NH3 plasma treatment and then reacted with triphosgene and Fmoc-protected hydrazine to yield a protected semicarbazide termination. Subsequent deprotection and chemical reaction with glyoxylyl peptides led to the covalent immobilization of the peptides on the surface through site-specific ligation. The resulting surfaces were characterized using X-ray photoelectron spectroscopy (XPS) and fluorescence measurements.
Coffinier
Yannick
Y
Institut de Recherche Interdisciplinaire (IRI, FRE2963) and Institut d'Electronique, de Microélectronique et de Nanotechnologie (IEMN), Cité Scientifique, Avenue Poincaré - BP. 60069, 59652 Villeneuve d'Ascq, France.
Szunerits
Sabine
S
Jama
Charafeddine
C
Desmet
Rémi
R
Melnyk
Oleg
O
Marcus
Bernadette
B
Gengembre
Léon
L
Payen
Edmond
E
Delabouglise
Didier
D
Boukherroub
Rabah
R
D000588:Amines / Q000737:chemistry*; D001895:Boron / Q000737:chemistry*; D002627:Chemistry, Physical / Q000379:methods; D018130:Diamond; D004566:Electrodes; D006859:Hydrogen / Q000737:chemistry; D008956:Models, Chemical; D010455:Peptides / Q000737:chemistry*; D040081:Protein Array Analysis; D012663:Semicarbazides / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013052:Spectrometry, X-Ray Emission; D013499:Surface Properties; D013696:Temperature
D000588:Amines; D010455:Peptides; D012663:Semicarbazides; D018130:Diamond; D006859:Hydrogen; D001895:Boron