Comparison of the measurement of lysosomal hydrolase activity in mycoplasma-contaminated and non-contaminated human fibroblast cultures treated with mycoplasma removal agent.
OBJECTIVE
To investigate the effect of both mycoplasma contamination and of its remover (MRA), through human fibroblasts culture over the activity of some lysosomal hydrolases.


METHODS
Activity was measured in contaminated fibroblasts before and after the addition of MRA. Results were compared with the enzymatic activity in control fibroblasts with and without MRA.


RESULTS
Only beta-glucosidase showed no significant alteration in the presence of either mycoplasma or MRA. Total hexosaminidase and beta-galactosidase underwent significant interference in the presence of the mycoplasma and the MRA. The % of hexosaminidase A and arylsulphatase A altered their activity only in the presence of MRA. Beta-glucuronidase changed its activity only in the presence of mycoplasma.


CONCLUSIONS
The fibroblast enzymes behaved differently in the presence of MRA and/or mycoplasma, demonstrating the sensitivity of these hydrolases. Our work suggests that mycoplasma and MRA alter the activity of some lysosomal hydrolases.
Souza
Fernanda Timm Seabra
FT
Departamento de Bioquímica, ICBS, UFRGS, Porto Alegre, RS, Brazil.
Souza Sostruznik
Luana
L
Casagrande Scolari
Roberta
R
Maciel de Castro
Karen Joana
KJ
Giugliani
Roberto
R
Coelho
Janice Carneiro
JC
D002478:Cells, Cultured; D004789:Enzyme Activation / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects* / Q000201:enzymology / Q000382:microbiology; D005959:Glucosidases / Q000378:metabolism; D005966:Glucuronidase / Q000378:metabolism; D006596:Hexosaminidases / Q000378:metabolism; D006801:Humans; D006867:Hydrolases / Q000378:metabolism*; D008247:Lysosomes / Q000201:enzymology*; D009174:Mycoplasma / Q000187:drug effects* / Q000254:growth & development; D015363:Quinolones / Q000494:pharmacology; D001616:beta-Galactosidase / Q000378:metabolism
D015363:Quinolones; C074448:mycoplasma removal agent; D006867:Hydrolases; D005959:Glucosidases; D006596:Hexosaminidases; D001616:beta-Galactosidase; D005966:Glucuronidase