Relation between the plasma levels of LDL-cholesterol and the expression of the early marker of inflammation long pentraxin PTX3 and the stress response gene p66ShcA in pacemaker-implanted patients.
Our goal was to set up a pilot study to explore the possible relation between the expression of p66((ShcA)) and PTX3, two emerging regulators of stress response and inflammation processes, respectively, and the circulating levels of LDL-cholesterol (LDL), a factor implicated in the development of inflammation and oxidative-stress associated diseases such as atherosclerosis. p66((ShcA)) and PTX3 mRNA contents were determined locally, in subcutaneous adipose specimens of non-diabetic pacemaker-implanted patients, and systemically in the circulating white blood cells (WBC) obtained from the same patients. The mean of the circulating LDL levels (125 mg/dl) was chosen as a threshold to identify two groups here considered to have high (>125 mg/dl) and low (<125 mg/dl) LDL plasma levels. Our data show that PTX3 and p66((ShcA)) mRNA levels are significantly more elevated in WBCs and in adipose tissue samples of patients with high levels of LDL compared to those with low levels. Additionally, a multiple regression analysis indicates that among LDL, TG, HDL, total cholesterol, CRP, creatinine and glucose levels, the only variable significantly affecting p66((ShcA)) and PTX3 mRNA expressions either in adipose tissue or in WBCs is represented by the circulating amount of LDL. In conclusion, our results suggest a potential link between the level of LDL and the expression of two genes involved in inflammation/oxidative stress pathways, i.e., p66((ShcA)) and PTX3, thus contributing to further understand the mechanism through which LDL may mediate the pathogenesis of inflammation and oxidative-stress associated diseases such as atherosclerosis.
Bosutti
A
A
Department of Clinical, Morfological and Technological Sciences, Division of Internal Medicine, University Hospital of Trieste, Cattinara, Strada di Fiume 447, I-34149, Trieste, Italy.
Grassi
G
G
Zanetti
M
M
Aleksova
A
A
Zecchin
M
M
Sinagra
G
G
Biolo
G
G
Guarnieri
G
G
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D000273:Adipose Tissue / Q000378:metabolism; D000368:Aged; D001483:Base Sequence; D015415:Biomarkers; D002097:C-Reactive Protein / Q000235:genetics*; D008078:Cholesterol, LDL / Q000097:blood*; D017931:DNA Primers / Q000235:genetics; D005260:Female; D006801:Humans; D007249:Inflammation / Q000209:etiology / Q000235:genetics / Q000378:metabolism; D007962:Leukocytes / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress; D010138:Pacemaker, Artificial; D010865:Pilot Projects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000683:Serum Amyloid P-Component / Q000235:genetics*; D055765:Shc Signaling Adaptor Proteins; D000071425:Src Homology 2 Domain-Containing, Transforming Protein 1; D000099153:Pentraxins
D048868:Adaptor Proteins, Signal Transducing; D015415:Biomarkers; D002097:C-Reactive Protein; D008078:Cholesterol, LDL; D017931:DNA Primers; D012333:RNA, Messenger; D000683:Serum Amyloid P-Component; D055765:Shc Signaling Adaptor Proteins; D000071425:Src Homology 2 Domain-Containing, Transforming Protein 1; D000099153:Pentraxins; C528024:SHC1 protein, human