title: D-mannose-modified iron oxide nanoparticles for stem cell labeling.
abstract: New surface-modified iron oxide nanoparticles were developed by precipitation of Fe(II) and Fe(III) salts with ammonium hydroxide according to two methods. In the first method, precipitation was done in the presence of D-mannose solution (in situ coating); the second method involved oxidation of precipitated magnetite with sodium hypochlorite followed by addition of D-mannose solution (postsynthesis coating). Selected nanoparticles were characterized by transmission electron microscopy (TEM), atomic force microscopy (AFM), elemental analysis, dynamic light scattering, infrared (IR), X-ray powder analysis, and ultrasonic spectrometry. While the first preparation method produced very fine nanoparticles ca. 2 nm in diameter, the second one yielded ca. 6 nm particles. Addition of D-mannose after synthesis did not affect the iron oxide particle size. UV-vis spectroscopy suggested that D-mannose suppresses the nonspecific sorption of serum proteins from DMEM culture medium on magnetic nanoparticles. Rat bone marrow stromal cells (rMSCs) were labeled with uncoated and d-mannose-modified iron oxide nanoparticles and with Endorem (Guerbet, France; control). Optical and transmission electron microscopy confirmed the presence of D-mannose-modified iron oxide nanoparticles inside the cells. D-mannose-modified nanoparticles crossed the cell membranes and were internalized well by the cells. Relaxivity measurements of labeled cells in gelatin revealed very high relaxivities only for postsynthesis D-mannose-coated iron oxide nanoparticles.
authors: Institute of Macromolecular Chemistry AS CR, Heyrovský Sq. 2, 162 06 Prague 6, Czech Republic. horak@imc.cas.cz
authors: Daniel
authors: D
authors: Horak
authors: Michal
authors: M
authors: Babic
authors: Pavla
authors: P
authors: Jendelová
authors: Vít
authors: V
authors: Herynek
authors: Miroslava
authors: M
authors: Trchová
authors: Zbynek
authors: Z
authors: Pientka
authors: Emil
authors: E
authors: Pollert
authors: Milan
authors: M
authors: Hájek
authors: Eva
authors: E
authors: Syková
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000648:ultrastructure; D005290:Ferric Compounds / Q000737:chemistry*; D008358:Mannose / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D010316:Particle Size; D051381:Rats; D012996:Solutions / Q000737:chemistry; D013057:Spectrum Analysis; D013194:Staining and Labeling / Q000379:methods*; D013234:Stem Cells / Q000648:ultrastructure*; D017154:Stromal Cells / Q000648:ultrastructure
chemical_list: D005290:Ferric Compounds; D012996:Solutions; C000499:ferric oxide; D008358:Mannose