Oxidative stress-inducible antioxidant adaptive response during prostaglandin F2alpha-induced luteal cell death in vivo.
Oxidative stress-induced antioxidant adaptive response would be particularly important to cells in high reactive oxygen species (ROS) environments. We aimed to determine the dynamic adaptive response of antioxidant enzymatic systems in sheep corpus luteum (CL) during PGF2alpha-induced luteal cell death. Activities of superoxide dismutase (SOD1 and SOD2), catalase (CAT), glutathione peroxidase (GPX) and glutathione reductase (GSR), and in situ DNA fragmentation were determined in CL at day 10 of the estrous cycle (0 h) and at 12, 24 or 48 h after PGF2alpha injection. A decrease in plasma progesterone concentration was first observed at 6 h after treatment (P < 0.05). Apoptotic cells were rarely observed in the CL at 0 h (less than 0.7%), and their incidence increased (P < 0.01) by 12 h post-PGF2alpha (11.7%) and remained thereafter elevated through 48 h. Activities of SOD1, SOD2, GPX and GSR were not changed at any time points after PGF2alpha treatment. CAT activity increased at 12 h (P < 0.01) and at 24 h (P < 0.05) after PGF2alpha treatment as compared to that at 0 h. These findings demonstrate that PGF2alpha induce luteal cell death without depressing the activity of antioxidant enzymes. It is suggested that transient increase in CAT activity is an adaptive response of the CL to oxidative stress induced by PGF2alpha.
Garrel
Catherine
C
Laboratoire de Biologie du Stress Oxydant, Département de Biologie Intégrée, Centre Hospitalier Universitaire de Grenoble, France.
Ceballos-Picot
Irene
I
Germain
Guy
G
Al-Gubory
Kaïs H
KH
D000222:Adaptation, Physiological; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D017209:Apoptosis; D002374:Catalase / Q000378:metabolism*; D015237:Dinoprost / Q000037:antagonists & inhibitors / Q000633:toxicity*; D005260:Female; D008184:Luteal Cells / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D018384:Oxidative Stress; D010088:Oxidoreductases / Q000378:metabolism; D012756:Sheep; D015854:Up-Regulation
D000975:Antioxidants; D015237:Dinoprost; D010088:Oxidoreductases; D002374:Catalase