Effects of two fluid resuscitations on the bacterial translocation and inflammatory response of small intestine in rats with hemorrhagic shock.
OBJECTIVE
To investigate the effects of two fluid resuscitations on the bacterial translocation and the inflammatory factors of small intestine in rats with hemorrhagic shock.


METHODS
Fifty SD healthy male rats were randomly divided into 5 groups (n equal to 10 per group): Group A (Sham group), Group B (Ringer's solution for 1 h), Group C (Ringer's solution for 24 h), Group D (hydroxyethyl starch for 1 h) and Group E ((hydroxyethyl starch for 24 h). A model of rats with hemorrhagic shock was established. The bacterial translocation in liver, content of tumor necrosis factor-alpha (TNF-alpha) and changes of myeloperoxidase enzyme (MPO) activities in small intestine were pathologically investigated after these two fluid resuscitations, respectively.


RESULTS
The bacterial translocation and the expression of TNF-alpha in the small intestine were detected at 1 h and 24 h after fluid resuscitation. There were significant increase in the number of translocated bacteria, TNF-alpha and MPO activities in Group C compared with Group B, significant decrease in Group E compared with Group D and in Group B compared with Group D. The number of translocated bacteria and TNF-alpha expression significantly decreased in Group E as compared with Group C.


CONCLUSIONS
The bacterial translocation and the expression of TNF-alpha in the small intestine exist 24 h after fluid resuscitation. 6% hydroxyethyl starch can improve the intestinal mucosa barrier function better than the Ringer's solution.
Gao
Xin-yao
XY
Department of Anesthesiology, the 4th Hospital of Shenzhen, Shenzhen 518033, China. gaodays@163.com
Ren
Cong-cai
CC
Zhou
Qiang
Q
Pang
Qing-feng
QF
Wu
Chang-yi
CY
Zeng
Yin-ming
YM
D000818:Animals; D018988:Bacterial Translocation / Q000187:drug effects*; D005440:Fluid Therapy; D006895:Hydroxyethyl Starch Derivatives / Q000008:administration & dosage / Q000494:pharmacology*; D007421:Intestine, Small / Q000378:metabolism*; D007552:Isotonic Solutions / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077331:Ringer's Solution; D012771:Shock, Hemorrhagic / Q000628:therapy*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D006895:Hydroxyethyl Starch Derivatives; D007552:Isotonic Solutions; D014409:Tumor Necrosis Factor-alpha; D000077331:Ringer's Solution; D009195:Peroxidase