title: Collagen distribution and expression of collagen-binding alpha1beta1 (VLA-1) and alpha2beta1 (VLA-2) integrins on CD4 and CD8 T cells during influenza infection.
abstract: Most viral infections occur in extralymphoid tissues, yet the mechanisms that regulate lymphocytes in these environments are poorly understood. One feature common to many extralymphoid environments is an abundance of extracellular matrix. We have studied the expression of two members of the beta(1) integrin family of collagen-binding receptors, alpha(1)beta(1) and alpha(2)beta(1) (CD49a, VLA-1 and CD49b, VLA-2, respectively), on CD4 and CD8 T cells during the response to influenza infection in the lung. Flow cytometry showed that whereas T cells infiltrating the lung and airways can express both CD49a and CD49b, CD49a expression was most strongly associated with the CD8+ subset. Conversely, though fewer CD4+ T cells expressed CD49a, most CD4+ cells in the lung tissue or airways expressed CD49b. This reciprocal pattern suggested that CD4 and CD8 T cells might localize differently within the lung tissue and this was supported by immunofluorescent analysis. CD8+ cells tended to localize in close proximity to the collagen IV-rich basement membranes of either the airways or blood vessels, whereas CD4+ cells tended to localize in the collagen I-rich interstitial spaces, with few in the airways. These observations suggest that CD4 T cell interaction with the tissue microenvironment is distinct from CD8 T cells and support the concept that CD4+ T cells in peripheral tissues are regulated differently than the CD8 subset.
authors: Department of Microbiology and Immunology, David H. Smith Center for Vaccine Biology and Immunology, Aab Institute of Biomedical Sciences, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA.
authors: Martin
authors: M
authors: Richter
authors: Steven J
authors: SJ
authors: Ray
authors: Timothy J
authors: TJ
authors: Chapman
authors: Sarah J
authors: SJ
authors: Austin
authors: Jonathan
authors: J
authors: Rebhahn
authors: Timothy R
authors: TR
authors: Mosmann
authors: Humphrey
authors: H
authors: Gardner
authors: Victor
authors: V
authors: Kotelianski
authors: Antonin R
authors: AR
authors: deFougerolles
authors: David J
authors: DJ
authors: Topham
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry* / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry* / Q000276:immunology; D003094:Collagen / Q000032:analysis* / Q000378:metabolism; D005260:Female; D039222:Integrin alpha1beta1 / Q000032:analysis*; D038982:Integrin alpha2beta1 / Q000032:analysis*; D008168:Lung / Q000737:chemistry / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D009976:Orthomyxoviridae Infections / Q000276:immunology*
chemical_list: D039222:Integrin alpha1beta1; D038982:Integrin alpha2beta1; D003094:Collagen