[Isolation and characterization of H2-producing strains Enterobacter sp. and Clostridium sp].
Two hydrogen-producing bacterial strains were newly isolated and identified as Enterobacter sp. Z-16 and Clostridium sp. C-32 by 16S rDNA sequence analysis. Various parameters for hydrogen production, including substrates, initial pH and temperature, have been studied. The optimum condition for hydrogen production of strain Z-16 were achieved as: initial pH7.0, temperature 35 degrees C , sucrose as the favorite substrate. In comparison, The optimum condition for hydrogen production of strain C-32 were obtained as: initial pH8.0, temperature 35 degrees C , maltose as the favorite substrate . Under batch fermentative hydrogen production conditions, the maximal hydrogen conversion rate for strain Z-16 and strain C-32 were 2.68 mol H2/mol sucrose and 2.71mol H2/mol maltose, respectively. Using glucose as substrate, the hydrogen conversion rate of strain Z-16 and strain C-32 were 2.35 and 2.48 mol H2/mol glucose, respectively. This research suggest a good application potential of strain Z-16 and C-32 in the future biological hydrogen production.
Zhi
Xiao-Peng
XP
School of Life Sciences, Bio-Energy Center, Xiamen University, Xiamen 361005, China.
Liu
Qing-Feng
QF
Wu
Xiao-Bing
XB
Xu
Hui-Juan
HJ
Long
Min-Nan
MN
D003013:Clostridium / Q000302:isolation & purification / Q000378:metabolism* / Q000648:ultrastructure; D004754:Enterobacter / Q000302:isolation & purification / Q000378:metabolism* / Q000648:ultrastructure; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008320:Maltose / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity; D013395:Sucrose / Q000378:metabolism; D013696:Temperature
D012336:RNA, Ribosomal, 16S; D013395:Sucrose; D008320:Maltose; D006859:Hydrogen; D005947:Glucose