title: Early social isolation decreases the expression of calbindin D-28k and dendritic branching in the medial prefrontal cortex of the rat.
abstract: Social isolation in rodents is the most well characterized animal model for early stressful experiences and their neurobehavioral consequences. The present study analyzed the effects of early social isolation on the expression of the calcium binding protein calbindin-D28k (CAD) and dendritic arborization in the medial prefrontal cortex (mPFC) of the rat. Sprague-Dawley male rats were reared either under isolation or social conditions from 21 to 51 postnatal days. At the end of this period the animals were behaviorally evaluated in the open-field test, sacrificed, and mPFC serial sections were processed either for immunocytochemical labeling against CAD or Golgi-Cox-Sholl staining. Isolated-reared rats exhibited a dramatic decrease in the number of CAD immunoreactive neurons and a significant dendritic atrophy of layer II/III pyramidal cells in association with a reduced exploratory behavior.
authors: Instituto de Ciencias Básicas, Universidad Católica del Maule, Talca, Chile. rpascual@ucm.cl
authors: Rodrigo
authors: R
authors: Pascual
authors: Pilar
authors: P
authors: Zamora-León
authors: Mitzi
authors: M
authors: Catalán-Ahumada
authors: Antoni
authors: A
authors: Valero-Cabré
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001522:Behavior, Animal; D064092:Calbindin 1; D064026:Calbindins; D003712:Dendrites / Q000502:physiology*; D005106:Exploratory Behavior / Q000502:physiology; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D017397:Prefrontal Cortex / Q000378:metabolism* / Q000648:ultrastructure*; D051381:Rats; D017207:Rats, Sprague-Dawley; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D012934:Social Isolation / Q000523:psychology*
chemical_list: C576500:Calb1 protein, rat; D064092:Calbindin 1; D064026:Calbindins; D064030:S100 Calcium Binding Protein G