title: Immunochemical method for sulfasalazine determination in human plasma.
abstract: Sulfasalazine is an antibiotic used in the treatment of inflammatory bowel diseases. For the assessment of sulfasalazine in several biological matrices, an Enzyme-Linked Immunosorbent Assay (ELISA) method based on polyclonal antibodies was developed and characterized. The immunoassay showed a high sensitivity (IC50=0.51 ng mL(-1)) and specificity, a detection limit of 0.02 ng mL(-1) and a dynamic range of 0.06-3.75 ng mL(-1) (80-20% inhibition). The immunoassay performed well when it was applied to spiked plasma samples (from 0.5 to 2.0 ng mL(-1)) previously cleaned up by protein precipitation with methanol. Recoveries ranged from 83 to 119%, with a mean value of 99% (CV=13%). Since sulfasalazine remaining of a treatment reaches the systemic circulation in unchanged form, the immunoassay can be applied to the determination of this pharmaceutical in human plasma in order to facilitate the control of the patients through the application of personal doses.
authors: Departamento de Química, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022 Valencia, Spain.
authors: Nuria
authors: N
authors: Pastor-Navarro
authors: Ester
authors: E
authors: Gallego-Iglesias
authors: Angel
authors: A
authors: Maquieira
authors: Rosa
authors: R
authors: Puchades
mesh_terms: D000818:Animals; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D011817:Rabbits; D012460:Sulfasalazine / Q000032:analysis / Q000097:blood* / Q000737:chemistry* / Q000276:immunology
chemical_list: D012460:Sulfasalazine