Analysis of laser-induced fluorescence spectra of in vitro plant tissue cultures.
We demonstrate the effectiveness of laser-induced fluorescence (LIF) for monitoring the development and stress detection of in vitro tissue cultures in a nondestructive and noninvasive way. The changes in LIF spectra caused by the induction of organogenesis, the increase of the F690/F740 ratio as a result of the stress originated in the organogenic explants due to shoot emergence, and the relationship between fluorescence spectra and shoot development were detected by LIF through closed containers of Saintpaulia ionantha.
Muñoz-Muñoz
Ana Celia
AC
Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Jalisco, México.
Gutiérrez-Pulido
Humberto
H
Rodríguez-Domínguez
José Manuel
JM
Gutiérrez-Mora
Antonia
A
Rodríguez-Garay
Benjamín
B
Cervantes-Martínez
Jesús
J
D002439:Cefotaxime / Q000494:pharmacology*; D007834:Lasers; D019684:Magnoliopsida / Q000187:drug effects / Q000378:metabolism*; D038081:Organogenesis / Q000187:drug effects / Q000502:physiology; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism*; D010940:Plant Proteins / Q000032:analysis* / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D010940:Plant Proteins; D002439:Cefotaxime