Intrinsic pro-angiogenic status of cystic fibrosis airway epithelial cells.
Cystic fibrosis is a common genetic disorder characterized by a severe lung inflammation and fibrosis leading to the patient's death. Enhanced angiogenesis in cystic fibrosis (CF) tissue has been suggested, probably caused by the process of inflammation, as similarly described in asthma and chronic bronchitis. The present study demonstrates an intrinsic pro-angiogenic status of cystic fibrosis airway epithelial cells. Microarray experiments showed that CF airway epithelial cells expressed several angiogenic factors such as VEGF-A, VEGF-C, bFGF, and PLGF at higher levels than control cells. These data were confirmed by real-time quantitative PCR and, at the protein level, by ELISA. Conditioned media of these cystic fibrosis cells were able to induce proliferation, migration and sprouting of cultured primary endothelial cells. This report describes for the first time that cystic fibrosis epithelial cells have an intrinsic angiogenic activity. Since excess of angiogenesis is correlated with more severe pulmonary disease, our results could lead to the development of new therapeutic applications.
Verhaeghe
Catherine
C
Unit of Human Genetics, GIGA-Research, University of Liège, Belgium.
Tabruyn
Sebastien P
SP
Oury
Cecile
C
Bours
Vincent
V
Griffioen
Arjan W
AW
D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D003550:Cystic Fibrosis / Q000473:pathology* / Q000503:physiopathology; D004847:Epithelial Cells; D048049:Extracellular Signal-Regulated MAP Kinases / Q000502:physiology; D006801:Humans; D009389:Neovascularization, Pathologic / Q000503:physiopathology*; D012137:Respiratory System / Q000166:cytology; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
D042461:Vascular Endothelial Growth Factor A; D048049:Extracellular Signal-Regulated MAP Kinases