Comparison of proteomes in various human plasma preparations by two-dimensional gel electrophoresis.
Serum or plasma can be utilized in a variety of studies targeted toward the discovery of disease biomarkers. In this study, the proteome profiles of plasma samples prepared using various anticoagulants (EDTA, heparin or citrate), were compared with those of serum using two-dimensional electrophoresis (2-DE). Proteins which evidenced different levels in the plasma and serum were screened and identified using ESI-Q-TOF MS/MS. The proteins which became detectable after the removal of fibrinogen from serum were identified as pigment epithelial differentiating factor (four spots), fetuin-like protein, and the hemopexin precursor. In particular, three proteins, pre-serum amyloid P component, plasma glutathione peroxidase precursor, and tetranectin, evidenced increased volume intensity only in the plasma samples prepared with EDTA.
Kim
Hyun-Jung
HJ
School of Life Sciences and Biotechnology, Korea University, 1, 5-ka, Anam-dong, Sungbuk-ku, Seoul 136-701, Republic of Korea.
Kim
Mi-Ryung
MR
So
Eun-Jung
EJ
Kim
Chan-Wha
CW
D000328:Adult; D001798:Blood Proteins / Q000235:genetics* / Q000302:isolation & purification*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000302:isolation & purification; D007202:Indicators and Reagents; D007525:Isoelectric Focusing / Q000379:methods; D008297:Male; D010949:Plasma; D020543:Proteome; D044967:Serum; D012709:Serum Albumin / Q000302:isolation & purification
D001798:Blood Proteins; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D020543:Proteome; D012709:Serum Albumin