[Application of flow cytometry in diagnosis of T-cell rich diffuse large B-cell lymphoma].
OBJECTIVE
To investigate the application of flow cytometry in diagnosis of T-cell rich diffuse large B-cell lymphoma.


METHODS
Histopathologic features, immunohistochemical findings and flow cytometry results of three cases of T-cell rich diffuse large B-cell lymphoma were reviewed retrospectively.


RESULTS
In CD45-side scatter (SSC) dot plot of the first patient, two different CD45-positive lymphoid cell populations were identified. The bright population consisted of both T and B cells, with a T-cell predominance. The dim population consisted mainly of B cells which showed lambda light chain restriction. In the second patient, CD45-positive cells were subdivided into two groups according to CD45-SSC dot plot. The small population consisted of both T and B cells, with a T-cell predominance. The large population consisted mainly of B cells which showed kappa light chain restriction. In the third patient, CD19-positive cells were subdivided into two groups according to the expression of CD20 in CD19-CD20 dot plot. The CD20-positive population expressed both kappa and lambda light chains, while the CD20-negative population demonstrated kappa light chain restriction.


CONCLUSIONS
Neoplastic B cells can be distinguished from reactive lymphoid cells in T-cell rich diffuse large B-cell lymphoma by flow cytometry, according to a number of parameters which include intensity of antigen expression, loss of antigens, expression of non-B-cell lineage antigens, patterns of forward scatter (FSC) and/or SSC, and expression of immature B-cell antigens.
Xia
Cheng-qing
CQ
Department of Pathology, Beijing Chaoyang Hospital-Affiliate of the Capital University of Medical Sciences, Beijing 100020, China. xiachengqing@yeah.net
Qi
Man
M
Xu
Xian-fa
XF
Li
Li-hong
LH
Zhao
Hong-ying
HY
D000368:Aged; D018941:Antigens, CD19 / Q000378:metabolism; D018951:Antigens, CD20 / Q000378:metabolism; D003937:Diagnosis, Differential; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007146:Immunoglobulin lambda-Chains / Q000378:metabolism; D007150:Immunohistochemistry; D017493:Leukocyte Common Antigens / Q000378:metabolism*; D016403:Lymphoma, Large B-Cell, Diffuse / Q000175:diagnosis* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000378:metabolism / Q000473:pathology*
D018941:Antigens, CD19; D018951:Antigens, CD20; D007146:Immunoglobulin lambda-Chains; D017493:Leukocyte Common Antigens