title: Testing estrogenicity of known and novel (xeno-)estrogens in the MolDarT using developing zebrafish (Danio rerio).
abstract: The MolDarT is a novel short-term assay for testing mechanism-based molecular effects in developing zebrafish embryos. The objective of this study was to evaluate the inducibility of vitellogenin1 mRNA (Vtg1) by the estrogenically active compounds 17beta-Estradiol (E2), 17alpha-Ethinylestradiol (EE2), Nonylphenol (NP), Bisphenol A (BPA), Cyproconazol, and the suspected xeno-estrogen Atrazin in the MolDarT. Freshly fertilized zebrafish eggs were exposed semistatically for 120 h. Using reverse transcription real-time PCR, the relative abundance of Vtg1 was measured. For EE2 a dose-response relationship was established with EC50 = 60.7 ng/L (205 pM). Induction of Vtg1 was significant at concentrations of 84 pM EE2 (25 ng EE2/L) and above, 10 nM E2 (2.7 microg E2/L), 100 nM E2 (27 microg E2/L), 10 microM BPA (2280 microg BPA/L), and 15 microM BPA (3420 microg BPA/L). At NP concentrations of 0.75 microM (165 microg NP/L) and 1.5 microM (330 microg NP/L) Vtg1 was significantly down-regulated. Both atrazine and cyproconazol showed no effect on relative Vtg1 abundance. With this study we further characterize the MolDarT assay and show its applicability for effect screening of compounds.
authors: Eawag, Swiss Federal Institute of Aquatic Science and Technology, Environmental Toxicology, Ueberlandstrasse 133, 8600 Dübendorf, Switzerland.
authors: Jane
authors: J
authors: Muncke
authors: Marion
authors: M
authors: Junghans
authors: Rik I L
authors: RI
authors: Eggen
mesh_terms: D000818:Animals; D001280:Atrazine / Q000633:toxicity; D001559:Benzhydryl Compounds; D001681:Biological Assay; D004967:Estrogens / Q000633:toxicity*; D004997:Ethinyl Estradiol / Q000633:toxicity; D005659:Fungicides, Industrial / Q000633:toxicity; D006540:Herbicides / Q000633:toxicity; D010636:Phenols / Q000633:toxicity; D011960:Receptors, Estrogen / Q000378:metabolism; D014230:Triazoles / Q000633:toxicity; D014819:Vitellogenins / Q000378:metabolism*; D015027:Zebrafish / Q000196:embryology / Q000378:metabolism*; D000098826:Bisphenol A Compounds
chemical_list: D001280:Atrazine; D001559:Benzhydryl Compounds; D004967:Estrogens; D004997:Ethinyl Estradiol; D005659:Fungicides, Industrial; D006540:Herbicides; D010636:Phenols; D011960:Receptors, Estrogen; D014230:Triazoles; D014819:Vitellogenins; C093628:cyproconazole; C025256:nonylphenol; C006780:bisphenol A; D000098826:Bisphenol A Compounds