Development and validation of a multi-residue method for the detection of a wide range of hormonal anabolic compounds in hair using gas chromatography-tandem mass spectrometry.
The monitoring of anabolic steroid residues in hair is undoubtedly one of the most efficient strategies to demonstrate the long-term administration of these molecules in meat production animals. A multi-residue sample preparation procedure was developed and validated for 28 steroids. A 100 mg hair sample was grinded into powder and extracted at 50 degrees C with methanol. After acidic hydrolysis and extraction with ethyl acetate, phenolsteroids, such as estrogens, resorcyclic acid lactones and stilbens in one hand, are separated from androgens and progestagens in the other hand. Solid phase extractions were performed before applying a specific derivatisation for each compound sub-group. Detection and identification were achieved using gas chromatography-tandem mass spectrometry with acquisition in the selected reaction monitoring mode after electron ionisation. The method was validated according to the 2002/657/EC guideline. Decision limits (CCalpha) for main steroids were in the 0.1-10 microg kg(-1) range.
Rambaud
Lauriane
L
LABERCA, Ecole Nationale Vétérinaire de Nantes, Route de Gachet, BP50707, 44307 Nantes Cedex 3, France.
Monteau
Fabrice
F
Deceuninck
Yoann
Y
Bichon
Emmanuelle
E
André
François
F
Le Bizec
Bruno
B
D045930:Anabolic Agents / Q000032:analysis* / Q000737:chemistry; D000818:Animals; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D004583:Electrons; D005504:Food Analysis / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006197:Hair; D006801:Humans; D008460:Meat; D000432:Methanol / Q000737:chemistry; D019800:Phenol / Q000737:chemistry; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods; D013256:Steroids / Q000032:analysis; D015813:Substance Abuse Detection / Q000379:methods*
D045930:Anabolic Agents; D013256:Steroids; D019800:Phenol; D000432:Methanol