Saxatilin, a snake venom disintegrin, suppresses TNF-alpha-induced ovarian cancer cell invasion.
Saxatilin is a disintegrin known to inhibit tumor progression in vivo and in vitro. The role of saxatilin in cancer cell invasion was examined by a modified Boyden chamber assay in MDAH 2774 human ovarian cancer cell line. Saxatilin (50 nM) significantly inhibited cancer cell invasion induced by tumor necrosis factor-alpha (TNF-alpha). Saxatilin also reduced MMP-9 mRNA levels in cancer cells in a dose-dependent manner. In addition, TNF-alpha-induced MMP-9 activity was reduced by the treatment of saxatilin. These results indicate that transcriptional regulation of MMP-9 is an important mechanism for the tumor suppressive effects of saxatilin in MDAH 2774 human ovarian cancer cells.
Kim
Dong Seok
DS
Department of Biochemistry, College of Science, Yonsei University, Seoul 120-749, Korea.
Jang
Yoon-Jung
YJ
Jeon
Ok-Hee
OH
Kim
Doo-Sik
DS
D000818:Animals; D045744:Cell Line, Tumor; D019483:Disintegrins / Q000494:pharmacology*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D009361:Neoplasm Invasiveness; D010051:Ovarian Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D012910:Snake Venoms / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000494:pharmacology*
D019483:Disintegrins; D012910:Snake Venoms; D014409:Tumor Necrosis Factor-alpha; C459080:saxatilin; D020780:Matrix Metalloproteinase 9