[Influential factor analysis and improvement of cultural method of oral mucosal epithelial cells].
OBJECTIVE
To analyze influential factors of cultural method of human oral mucosal epithelial cells (hOMEC)and to establish a reliable cell culture system for hOMEC.


METHODS
Benzalkonium bromide and gentamicin sulfate were used to prevent microbial contamination. Separations of epithelium from underlying connective tissues with Dispase at different concentration were compared. Enzyme digestion was used to isolate cells and keratinocyte-serum free medium(K-SFM) was employed for primary culture and subculture of hOMEC.


RESULTS
Microbial contamination was under control. Separation of epithelium from underlying connective tissues with 0.40% Dispase was more complete than that of 0.25% Dispase. The cells grew fast and well in vitro.


CONCLUSIONS
The high successful culture of hOMEC and simplified procedures could be obtained with improvement of methods.
Wang
Xiang
X
Dept. of Oral Medicine, School of Stomatology, Jilin University, Changchun 130041, China.
Gao
Wen-xin
WX
Wang
Li
L
Wei
Xiu-feng
XF
Chen
Ying-xin
YX
Wang
Xiao-feng
XF
D018929:Cell Culture Techniques; D004847:Epithelial Cells; D004848:Epithelium; D005163:Factor Analysis, Statistical; D006801:Humans; D015603:Keratinocytes; D009061:Mouth Mucosa