[Update of the researches on sperm proteome].
Proteomics and polemic techniques are among the most valuable approaches in the research of life science in this new century, marking the beginning of a post-genome era. Two-dimensional electrophoresis and mass spectrometry, applied as key techniques in proteomic research, have given rise to new research strategies and improved the efficiency of researchers in exploring the unknown field. Its introduction into the exploration of spermatozoal proteins has given us so many pleasant surprises. This review presents some essential information about proteomics and two-dimensional electrophoresis and mass spectrometry, with a brief introduction of the recent progress in the researches on human sperm proteome, capacitation-related sperm proteins, sperm-egg interaction-related proteins, and sperm-immunity which has made great senses in the clinical problems.
Wang
Yi
Y
First School of Clinical Medicine, Nanjing Medical University, Key Laboratory of Reproductive Medicine of Jiangsu Province, Nanjing, Jiangsu 210029, China. anu_yi@hotmail.com
Zhou
Zuo-min
ZM
D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008297:Male; D020543:Proteome; D013094:Spermatozoa / Q000276:immunology
D020543:Proteome