[The effect of NT4-NAP fusion gene transfection on photoprotection of rabbit iris pigment epithelium cells].
OBJECTIVE
To investigate the effect of infection with adeno-associated virus (AAV) vector containing NT4-NAP fusion gene on photoprotection of rabbit iris pigment epithelium cells (IPECs).


METHODS
rAAV-GFP and rAAV-NAP (containing NT4-NAP fusion gene) were constructed; rabbit IPECs were cultured and infected with rAAV-GFP and rAAV-NAP; transfection of viruses was detected by GFP fluorescence expression; NAP protect rabbit iris pigment epithelium from light stimulation was evaluated by MTT and flow cytometry.


RESULTS
Rabbit IPECs expressed the GFP fluorescence; comparing with control cells, IPECs transfected with rAAV-NAP remained normal proliferation and showed lower apoptosis percentage after ultra-violet stimulation.


CONCLUSIONS
rAAV-NAP constructed in the study can infect rabbit IPECs, and NAP may protect rabbit IPECs from ultra-violet damage.
She
Hua-ning
HN
Department of Ophthalmology, the Second Hospital, Xi'an Jiaotong University, Xi'an 710004, China. shehuaning@126.com
Sun
Nai-xue
NX
Zheng
Yu-ping
YP
Quan
Yan-long
YL
Cao
Zhen-ping
ZP
Wang
Xiao-hua
XH
D000818:Animals; D002478:Cells, Cultured; D050939:Gene Fusion; D005822:Genetic Vectors; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009842:Oligopeptides / Q000235:genetics*; D010857:Pigment Epithelium of Eye / Q000166:cytology*; D011817:Rabbits; D014162:Transfection
D009419:Nerve Tissue Proteins; D009842:Oligopeptides; C425904:davunetide