[Expression of p53 gene with DNA polbeta and CMV promoter in salivary adenoid cystic carcinoma cells].
OBJECTIVE
To evaluate the activity of DNA polbeta promoter on p53 gene in salivary adenoid cystic carcinoma (SACC) cells.


METHODS
The luciferase activity was examined and used to evaluate the activity of DNA polbeta promoter on SACC-83 cells. Eukaryotic expression plasmids of p53 gene were constructed and stably transfected into SACC-83 cells. RT-PCR was used to assess the expression of p53 gene. The SACC-83 cells were subjected to the treatments of H2O2, ultraviolet radiation, Bleocin, and affected p53 mRNA and protein level in SACC-83 cells were characterized with RT-PCR and Western blotting.


RESULTS
The result of luciferase activity proved that the activity of DNA polbeta promoter in SACC-83 cells was much higher than that of CMV promoter. The results of RT-PCR suggested that p53 gene with different promoters were all expressed effectively, but the expression efficiency was different. It was greater in DNA polbeta group than in CMV group. After DNA damage, p53 gene expression increased and DNA polbeta promoter could enhance the expression of p53 gene more than CMV promoter. The results of Western blotting indicated that the expression of P53 protein between the two groups did not show any difference.


CONCLUSIONS
In SACC cells, the activity of DNA polbeta promoter was increased and DNA polbeta promoter could enhance the expression of p53.
Yan
Bing-zhi
BZ
Dept. of Oral Pathology, College of Stomatology, Hebei Medical University, Shijiazhuang 050017, China.
Wang
Jie
J
Zhang
Bo
B
Dong
Fu-sheng
FS
Hou
Lin
L
Wang
Xu
X
D003528:Carcinoma, Adenoid Cystic; D045744:Cell Line, Tumor; D004247:DNA; D016158:Genes, p53; D006801:Humans; D006861:Hydrogen Peroxide; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger; D012468:Salivary Gland Neoplasms; D014162:Transfection; D014466:Ultraviolet Rays
D012333:RNA, Messenger; D004247:DNA; D006861:Hydrogen Peroxide