The use of a combined enrichment-filtration technique for the isolation of Campylobacter spp. from clinical samples.
A combined non-selective enrichment-filtration technique was investigated for the isolation of Campylobacter spp. from clinical samples. In total, 479 samples were tested by direct culture, enrichment subculture and enrichment-filtration. The enrichment-filtration technique was used with both selective and non-selective media. Direct culture and enrichment subculture yielded 13 and seven isolates, respectively, while enrichment-filtration yielded 18 and 14 isolates on selective and non-selective agar, respectively. Thus, the combination of enrichment-filtration with selective agar produced a 38.5% increase in the number of isolates (p <0.05). All isolates were identified as Campylobacter jejuni.
Wilson
G
G
Department of Medical Microbiology, Stirling Royal Infirmary, Stirling, UK. gwilson44@btinternet.com
Aitchison
L B
LB
D001431:Bacteriological Techniques / Q000379:methods*; D012634:Bodily Secretions / Q000382:microbiology; D002167:Campylobacter / Q000254:growth & development / Q000302:isolation & purification*; D002169:Campylobacter Infections / Q000382:microbiology*; D003470:Culture Media; D005374:Filtration / Q000379:methods*; D006801:Humans
D003470:Culture Media