title: Improved quantitative method for G6PD deficiency detection.
abstract: Glucose-6-phosphate dehydrogenase (G6PD) deficiency is a common hereditary enzyme defect that affects over 400 million people worldwide. Deficiency in G6PD can cause hemolytic anemia, which can be fatal if it is not treated properly. Mass qualitative G6PD-deficiency screening methods have been available for some time; however, in this study we developed the first high-throughput quantitative method for measuring G6PD activity. A patient's blood can be analyzed directly from the original collection tubes on a Hitachi P modular serum work area (SWA), which dramatically reduces the analysis time and the cost of the assay. The results presented here may enable the development of other improved, automated assays of whole-blood samples.
authors: Reference Chemistry Laboratory, Department of Pathology and Area Laboratory Services, Brooke Army Medical Center, Ft. Sam Houston, Texas 78234, USA. yunhua.fan@us.army.mil
authors: Yun-Hua
authors: YH
authors: Fan
authors: LuAnn
authors: L
authors: Lazenbery
authors: Elizabeth
authors: E
authors: Foster
authors: Felix
authors: F
authors: Duelm
authors: Earl
authors: E
authors: Grant
mesh_terms: D019411:Clinical Laboratory Techniques / Q000191:economics / Q000379:methods*; D005954:Glucosephosphate Dehydrogenase / Q000097:blood*; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000175:diagnosis* / Q000201:enzymology; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D008403:Mass Screening / Q000191:economics / Q000379:methods*; D012016:Reference Values; D015203:Reproducibility of Results
chemical_list: D006454:Hemoglobins; D005954:Glucosephosphate Dehydrogenase