Betanin inhibits the myeloperoxidase/nitrite-induced oxidation of human low-density lipoproteins.
Production of nitrogen dioxide by the activity of myeloperoxidase (MPO) in the presence of nitrite is now considered a key step in the pathophysiology of low-density lipoprotein (LDL) oxidation. This study shows that betanin, a phytochemical of the betalain class, inhibits the production of lipid hydroperoxides in human LDL submitted to a MPO/nitrite-induced oxidation. Kinetic measurements including time-course of particle oxidation and betanin consumption, either in the presence or in the absence of nitrite, suggest that the antioxidant effect is possibly the result of various actions. Betanin scavenges the initiator radical nitrogen dioxide and can also act as a lipoperoxyl radical-scavenger. In addition, unidentified oxidation product(s) of betanin by MPO/nitrite inhibit(s) the MPO/nitrite-induced LDL oxidation as effectively as the parent compound. In the light of betanin bioavailability and post-absorbtion distribution in humans, present findings may suggest favourable in vivo activity of this phytochemical.
Allegra
Mario
M
Department of Pharmaceutical, Toxicological and Biological Chemistry, Università di Palermo, Italy.
Tesoriere
Luisa
L
Livrea
Maria A
MA
D050859:Betacyanins / Q000737:chemistry / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008077:Lipoproteins, LDL / Q000737:chemistry*; D009573:Nitrites / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D009585:Nitrogen Dioxide / Q000737:chemistry; D010084:Oxidation-Reduction / Q000187:drug effects; D009195:Peroxidase / Q000037:antagonists & inhibitors* / Q000494:pharmacology
D050859:Betacyanins; D016166:Free Radical Scavengers; D008077:Lipoproteins, LDL; D009573:Nitrites; C020228:betanin; D009195:Peroxidase; D009585:Nitrogen Dioxide