[The genetic polymorphism of cytokine genes in Zhejiang Han individuals].
In order to investigate the genetic polymorphism of 13 cytokines genes in Han individuals from Zhejiang. The polymerase chain reaction-sequence specific primers (PCR-SSP) method was used to determine genetic polymorphisms in 100 unrelated healthy Han individuals from Zhejiang province. We found that: (1) The frequency of the TGFbeta (G codon 25) allele is 1.00 and no TGFbeta (C codon 25) allele is found. (2) The frequencies of IL-1alpha(C-889), IL-1beta(C +3962), IL-4 (T-1098), IL-6 (G-174), IL-6 (G nt565), IL-10 (A-1082) and TNFalpha (G-238) alleles are more than 0.95; while the frequencies of IL-1alpha (T-889), IL-1alpha (T +3962), IL-4 (G-1098), IL-6 (A-174), IL-6 (A nt565), IL-10 (G-1082), TNFalpha (A-238) alleles are less than 0.05. (3) The haplotype frequency of the high-producer-type GCC in IL-10 gene is 0.05, while the low-producer-type ATA is 0.735. The haplotype frequency of the high-producer-type TG in the TGFbeta gene is 0.49, while the low-producer-type CC is 0. The haplotype frequency of the high-producer-types AG and AA in the TNFalpha gene is 0.055, while of the low-producer-types GG and GA is 0.945. The results showed that the genetic polymorphism of cytokine genes in Han individuals from Zhejiang Province is distinctive.
Zhang
Wei
W
Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research of Ministry of Health, Hangzhou, China. qxk@zjb.org.cn
Zhu
Hong
H
Lv
Qin-Feng
QF
Wang
Wei
W
Han
Zhe-Dong
ZD
Zhu
Fa-Ming
FM
Yan
Li-Xing
LX
D002681:China; D016207:Cytokines / Q000235:genetics*; D005787:Gene Frequency; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics; D053582:Interleukin-1alpha / Q000235:genetics; D053583:Interleukin-1beta / Q000235:genetics; D015847:Interleukin-4 / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D011110:Polymorphism, Genetic / Q000235:genetics*; D016212:Transforming Growth Factor beta / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D016207:Cytokines; D053582:Interleukin-1alpha; D053583:Interleukin-1beta; D015850:Interleukin-6; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D015847:Interleukin-4