Association analysis of hepatitis virus B infection with haplotypes of the TBX21 gene promoter region in the Chinese population.
BACKGROUND
The T-box21 (TBX21) gene encodes the transcription factor T-bet (T-box expressed in T-cells), which influences naive T-lymphocyte development and has been implicated in the pathogenesis of many diseases.


METHODS
We selected 208 hepatitis B patients and 213 healthy volunteers to examine whether polymorphisms or haplotypes of the TBX21 gene promoter were associated with hepatitis B virus (HBV) infection in the Chinese population. Two polymorphisms at -1499 and -1514 located in the TBX21 promoter region were identified by the PCR-restriction fragment length polymorphism (PCR-RFLP) method.


RESULTS
Single nucleotide polymorphism (SNP) at -1499 was significantly different between HBV patients and healthy controls [p=0.003; odds ratio (OR) 3.65, 95% confidence interval (CI) 1.58-8.45]. Similarly, our results showed a significantly higher level of haplotype D (--/AC) in HBV patients compared to control subjects (p=0.005; OR 4.82, 95% CI 1.59-14.61).


CONCLUSIONS
Based on our findings, it seems that genetic variations of allele -1499 and haplotype D (--/AC) within the TBX21 promoter region contribute to susceptibility to HBV infection in the Chinese population.
Cao
Bangwei
B
Department of Anti-infection and Institute of Clinical Pharmacology, Peking University First Hospital, Beijing, China.
Yang
Hongli
H
Ding
Howard
H
Qi
Suxia
S
Gao
Lei
L
Cui
Hong
H
Dai
Yue
Y
Xu
Changqing
C
D044466:Asian People; D016022:Case-Control Studies; D002681:China; D004247:DNA / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006509:Hepatitis B / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D012016:Reference Values; D020825:T-Box Domain Proteins / Q000235:genetics*; D000098815:T-bet Transcription Factor
D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D004247:DNA