title: Colorimetric detection and fingerprinting of bacteria by glass-supported lipid/polydiacetylene films.
abstract: Glass-supported films of lipids and polydiacetylene were applied for visual detection and colorimetric fingerprinting of bacteria. The sensor films comprise polydiacetylene domains serving as the chromatic reporter interspersed within lipid monolayers that function as a biomimetic membrane platform. The detection schemes are based on either visible blue-red transitions or fluorescence transformations of polydiacetylene, induced by amphiphilic molecules secreted by proliferating bacteria. An important feature of the new film platform is the feasibility of either naked-eye detection of bacteria or color analysis using conventional scanners. Furthermore, we find that the degrees of bacterially induced color transformations depend both on the bacterial strains examined and the lipid compositions of the films. Accordingly, bacterial fingerprinting can be achieved through pattern recognition obtained by recording the chromatic transformations in an array of lipid/PDA films having different lipid components.
authors: Department of Chemistry and the Ilse Katz Center for Nanotechnology, Ben Gurion University, Beer Sheva, Israel 84105.
authors: Yogesh
authors: Y
authors: Scindia
authors: Liron
authors: L
authors: Silbert
authors: Roman
authors: R
authors: Volinsky
authors: Sofiya
authors: S
authors: Kolusheva
authors: Raz
authors: R
authors: Jelinek
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D015373:Bacterial Typing Techniques / Q000379:methods*; D032701:Biomimetics; D003124:Colorimetry / Q000379:methods*; D005898:Glass; D008055:Lipids / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D010363:Pattern Recognition, Automated; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry*; D053279:Polyynes / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
chemical_list: D008055:Lipids; D000078789:Polyacetylene Polymer; D011108:Polymers; D053279:Polyynes; C082361:polydiacetylene