title: The role of pannexin 1 hemichannels in ATP release and cell-cell communication in mouse taste buds.
abstract: ATP has been shown to be a taste bud afferent transmitter, but the cells responsible for, and the mechanism of, its release have not been identified. Using CHO cells expressing high-affinity neurotransmitter receptors as biosensors, we show that gustatory stimuli cause receptor cells to secrete ATP through pannexin 1 hemichannels in mouse taste buds. ATP further stimulates other taste cells to release a second transmitter, serotonin. These results provide a mechanism to link intracellular Ca(2+) release during taste transduction to secretion of afferent transmitter, ATP, from receptor cells. They also indicate a route for cell-cell communication and signal processing within the taste bud.
authors: Department of Physiology and Biophysics, Miller School of Medicine, University of Miami, Miami, FL 33136, USA.
authors: Yi-Jen
authors: YJ
authors: Huang
authors: Yutaka
authors: Y
authors: Maruyama
authors: Gennady
authors: G
authors: Dvoryanchikov
authors: Elizabeth
authors: E
authors: Pereira
authors: Nirupa
authors: N
authors: Chaudhari
authors: Stephen D
authors: SD
authors: Roper
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D016466:CHO Cells; D002118:Calcium / Q000378:metabolism; D002450:Cell Communication / Q000502:physiology*; D017630:Connexins; D006224:Cricetinae; D003412:Cricetulus; D017403:In Situ Hybridization; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012701:Serotonin / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D013650:Taste Buds / Q000502:physiology*
chemical_list: D017630:Connexins; D009419:Nerve Tissue Proteins; C503631:Panx1 protein, mouse; D012701:Serotonin; D000255:Adenosine Triphosphate; D002118:Calcium