title: A beta-1,3-galactosyltransferase and brainiac/bre5 homolog expressed in the midgut did not contribute to a Cry1Ab toxin resistance trait in Ostrinia nubilalis.
abstract: Post-translational glycosylation of midgut epithelial protein and lipid receptors may be required prior to binding of activated Bacillus thuringiensis (Bt) Cry toxins. A 931bp cDNA encoding a putative 297-residue beta-1,3-galactosyltransferase (beta3GalT5) was cloned from larval Ostrinia nubilalis midgut tissue, and showed homology to Drosophila brainiac (brn) and Caenorhabditis elegans bre5 proteins. Single nucleotide polymorphisms (SNPs) were detected in coding and promoter regions of O. nubilalis beta3GalT5 (Onb3GalT5), of which 3 of 31 CDS SNPs were non-synonymous. SNPs within HaeIII and MspI recognition sites were confirmed by PCR-RFLP, and are Mendelian inherited. Analysis of F(2) pedigrees suggested an Onb3GalT5 SNP C660 fixed within a Cry1Ab-resistant colony was not correlated with Cry1Ab resistance traits, as measured by higher larval O. nubilalis weights when fed toxin-containing diet.
authors: USDA-ARS, Corn Insect and Crop Genetics Research Unit, Genetics Laboratory, Iowa State University, Ames, IA 50011, USA. coates@iastate.edu <coates@iastate.edu>
authors: Brad S
authors: BS
authors: Coates
authors: Douglas V
authors: DV
authors: Sumerford
authors: Richard L
authors: RL
authors: Hellmich
authors: Leslie C
authors: LC
authors: Lewis
mesh_terms: D020121:5' Untranslated Regions; D000595:Amino Acid Sequence; D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D001483:Base Sequence; D001835:Body Weight; D001921:Brain / Q000201:enzymology; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry; D004032:Diet; D029721:Drosophila Proteins / Q000737:chemistry; D004731:Endotoxins; D005260:Female; D005700:Galactosyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005787:Gene Frequency; D006460:Hemolysin Proteins; D007305:Insecticide Resistance / Q000235:genetics; D007422:Intestines / Q000201:enzymology; D007814:Larva / Q000201:enzymology; D008297:Male; D008565:Membrane Proteins / Q000737:chemistry; D008969:Molecular Sequence Data; D009036:Moths / Q000201:enzymology* / Q000235:genetics; D010641:Phenotype; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
chemical_list: D020121:5' Untranslated Regions; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D029742:Caenorhabditis elegans Proteins; D029721:Drosophila Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; D008565:Membrane Proteins; C103963:brn protein, Drosophila; C030956:insecticidal crystal protein, Bacillus Thuringiensis; C433229:BRE-5 protein, C elegans; D005700:Galactosyltransferases