Study of biocompatibility of small intestinal submucosa (SIS) with Schwann cells in vitro.
No satisfactory method currently exists for repairing long peripheral nerve defects. Efforts have been made to fabricate bioactive artificial nerve conduits, comprised of a biomaterial pre-seeded with Schwann cells (SCs), which creating a favorable micro-environment for axonal regeneration, to be an alternative to autografting by means of tissue engineering. Small intestinal submucosa (SIS) possesses special biological characteristics and is comprehensively researched for tissue repairing at varied tissues and organs. This study investigated the biocompatibility of SIS with SCs in vitro. Cultured rat SCs were seeded on SIS. Cell morphology was observed by light microscopy, scanning electron microscopy and transmission electron microscope. The viability of SCs was measured by MTT assay. Secretion of NGF-beta and BDNF was quantitatively assessed by ELISA, and NGF-beta mRNA and BDNF mRNA were semi-quantitatively assessed by RT-PCR. The results indicated that SCs could adhere, migrate and proliferate on the surface of SIS in good condition with productive function of secreting growth factors. SIS has a good biocompatibility with SCs and SIS pre-seeded with SCs has potential to be an alternate candidate of autografting for repairing long peripheral nerve defects.
Su
Yan
Y
Department of Orthopaedics, The Sixth Affiliated People's Hospital, Shanghai Jiaotong University, 600 Yishan Road, Shanghai 200233, China.
Zeng
Bing-Fang
BF
Zhang
Chang-Qing
CQ
Zhang
Kai-Gang
KG
Xie
Xue-Tao
XT
D000818:Animals; D000831:Animals, Newborn; D001672:Biocompatible Materials / Q000627:therapeutic use*; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D006085:Graft Survival / Q000502:physiology; D007413:Intestinal Mucosa / Q000502:physiology* / Q000648:ultrastructure; D007421:Intestine, Small / Q000502:physiology / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D020932:Nerve Growth Factor / Q000235:genetics / Q000378:metabolism; D009416:Nerve Regeneration / Q000502:physiology; D010525:Peripheral Nerves / Q000166:cytology / Q000502:physiology* / Q000601:surgery; D010523:Peripheral Nervous System Diseases / Q000628:therapy*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000378:metabolism* / Q000648:ultrastructure; D034421:Sus scrofa; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D019208:Brain-Derived Neurotrophic Factor; D012333:RNA, Messenger; D020932:Nerve Growth Factor