Excretion profile of boldenone in urine of veal calves fed two different milk replacers.
The residue profiles of 17alpha-/17beta-boldenone conjugated (17alpha/beta-Bol) and ADD were investigated by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in urine of male veal calves fed two commercial milk replacers, with different content of cholesterol and phytosterols. The urine samples were collected within 4 h after feeding and further from all the animals. Detectable amounts of 17alpha-Bol conjugated were measured in urine collected from all calves, but the concentrations of 17alpha-Bol were higher in urine from calves receiving the milk replacer with the greater amount of phytosterols. During the whole experiment, 17beta-Bol and ADD were never detected in urine samples collected.
Draisci
R
R
Istituto Superiore di Sanità, Department of Food Safety and Veterinary Public Health, Viale Regina Elena 299, 00161 Roma, Italy.
Merlanti
R
R
Ferretti
G
G
Fantozzi
L
L
Ferranti
C
C
Capolongo
F
F
Segato
S
S
Montesissa
C
C
D045930:Anabolic Agents / Q000032:analysis* / Q000652:urine*; D000821:Animal Feed; D000818:Animals; D002138:Calibration; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods*; D004032:Diet; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D008892:Milk / Q000737:chemistry*; D008956:Models, Chemical; D010840:Phytosterols / Q000737:chemistry; D013261:Sterols / Q000737:chemistry; D013739:Testosterone / Q000031:analogs & derivatives* / Q000032:analysis / Q000652:urine; D013997:Time Factors
D045930:Anabolic Agents; D010840:Phytosterols; D013261:Sterols; D013739:Testosterone; C013915:boldenone