[Study on SNPs of GHR gene and its associations with growth traits in Qinchuan cattle].
In this paper PCR-SSCP technique was used to analyze the polymorphisms of the tenth exon of GHR gene in Qinchuan cattle. The results showed that PCR products demonstrated polymorphisms in Qinchuan cattle and the population was at Hardy-Weinberg equilibrium (P > 0.05). There were three allele gene A, B, C in this locus with the corresponding frequencies of 0.5956, 0.2905 and 0.1140. The amplified fragments of homozygosis genotype were sequenced. The result showed that there were two mutation in these fragments. Then the relationship between genotypes and productive performances was analyzed by the Least Squire Method (LSM). The results showed that some productive performances of the indi-viduals with genotype AB were higher than those of the other genotypes, and the derivation was significant among geno-types. It suggested that GHR gene may be a candidate gene responsible for growth trait in Qinchuan cattle.
Zhao
Gao-Feng
GF
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, China. zhaochenfeng122@163.com
Chen
Hong
H
Lei
Chu-Zhao
CZ
Zhang
Chun-Lei
CL
Zhang
Li
L
Lan
Xian-Yong
XY
Fang
Xing-Tang
XT
Kang
Xiang-Tao
XT
D000483:Alleles; D000818:Animals; D002417:Cattle; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006128:Growth / Q000235:genetics*; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational; D011986:Receptors, Somatotropin / Q000235:genetics* / Q000378:metabolism
D011986:Receptors, Somatotropin