Transgenerational effect of neonatal vitamin A or D treatment (hormonal imprinting) on the hormone content of rat immune cells.
Male offspring of neonatally vitamin A or D treated (hormonally imprinted) rat dams were studied for hormone (adrenocorticotrophine [ACTH], beta-endorphin, histamine, triiodothyronine [T3]) content in immune cells, by using immunocytochemical methods for flow cytometry and confocal microscopy. ACTH and T3 were almost doubled in the lymphocytes of vitamin A treated mothers' offspring, while histamine decreased to a one-third in the histamine content of vitamin D treated mothers' offspring. Part of the animals received vitamin treatment again 24 hours before measurement, however, only endorphin content elevated moderately. In the offspring of untreated dams administered with vitamin D 24 hours before measurement, each cell type studied (lymphocyte, monocyte-granulocyte group, mast cell) had a one-third lower T3 content, which shows that vitamin D treatment can influence hormone content of immune cells. The experiments call attention to the transgenerational effect of perinatal treatment with lipid-soluble, intracellular receptor-bound vitamins.
Csaba
G
G
Department of Genetics, Cell and Immunobiology, Semmelweis University, Budapest, Hungary. csagyor@dgci.sote.hu
Kovács
P
P
Pállinger
E
E
D000818:Animals; D000831:Animals, Newborn; D005191:Family Characteristics; D005260:Female; D006728:Hormones / Q000378:metabolism*; D007962:Leukocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014801:Vitamin A / Q000494:pharmacology*; D014807:Vitamin D / Q000494:pharmacology*
D006728:Hormones; D014801:Vitamin A; D014807:Vitamin D