NK-KIR ligand identification: a quick Q-PCR approach for HLA-C epitope typing.
Interaction of donor natural killer (NK)-cell-associated killer cell immunoglobulin-like receptors (KIRs) with the patient's human leukocyte antigen-C (HLA-C) ligands can result in an alloreactive NK response after haematopoietic stem cell transplantation. In many retrospective studies, additional HLA-C-typing data are required to predict NK-cell alloreactivity. We developed a Taqman assay using the quantitative polymerase chain reaction (Q-PCR) technique that facilitates HLA-C epitope typing, allowing the assignment of HLA-C group 1 or 2 alleles based on the dimorphism at residues 77 and 80 rather than based on the sequence specific priming (SSP) and sequence-based typing allele types. Q-PCR analysis for HLA-C epitope detection showed three clusters reflecting homozygous group 1 or 2 and heterozygous samples. This new approach introduces a quick HLA-C epitope screening method to define the presence of the ligand for the KIR-HLA-C interaction.
Schellekens
J
J
Department of Pathology, University Medical Centre Utrecht, Utrecht, The Netherlands.
Rozemuller
E H
EH
Borst
H P E
HP
Otten
H G
HG
van den Tweel
J G
JG
Tilanus
M G J
MG
D000483:Alleles; D017931:DNA Primers / Q000737:chemistry; D000939:Epitopes / Q000235:genetics*; D006680:HLA Antigens / Q000235:genetics; D015236:HLA-C Antigens / Q000096:biosynthesis* / Q000235:genetics*; D006648:Histocompatibility; D006650:Histocompatibility Testing / Q000379:methods*; D006720:Homozygote; D006801:Humans; D007694:Killer Cells, Natural / Q000166:cytology*; D008024:Ligands; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers; D000939:Epitopes; D006680:HLA Antigens; D015236:HLA-C Antigens; D008024:Ligands