title: Expression of adhesion factors induced by epileptiform activity in the endothelium of the isolated guinea pig brain in vitro.
abstract: OBJECTIVE
  Brain inflammation has been recently considered in the pathogenesis of focal epilepsies. Synthesis of pro-inflammatory mediators in the brain was described both in experimental models of seizures and in human postsurgical tissue. Inflammatory mediators may up-regulate endothelial adhesion molecules, therefore promoting adhesion and homing of leucocytes into the brain. In the present study, expression of inducible adhesion factors in brain endothelium was verified after pharmacological induction of seizure-like activity in specific brain areas of the in vitro isolated guinea pig brain.
  
  
  METHODS
  Experiments were performed in isolated guinea-pig brains maintained in vitro by arterial perfusion. In this preparation, brief application of the GABAa receptor-antagonist, bicuculline, consistently induced focal ictal discharges in the limbic region that secondarily diffuse to the neocortex, as verified by simultaneous electrophysiological recording of extracellular activity. At the end of the electrophysiological experiment (after 5 h in vitro), brains were fixed and immunostaining for adhesion molecules P-selectin and ICAM-1 and for Fos protein was evaluated.
  
  
  RESULTS
  Immunohistochemical analysis of isolated brains in which seizure-like activity was induced revealed expression of inducible adhesion factors P-selectin and ICAM-1 in the endothelium of small-medium size brain vessels. In particular, the expression of these molecules was consistently observed in all areas involved in epileptic seizure-like ictal activity (limbic cortices and neocortex), and was infrequently found in regions that generated interictal spiking (piriform cortex), suggesting a trigger role played by seizures for endothelial activation. An increase in Fos protein expression was evident in all analyzed limbic areas and in the neocortex, indicating a correlation between the areas of neuronal and endothelial activation. In control brains maintained in vitro for comparable times without induction of epileptiform activity, no immunoreactivity for Fos and adhesion molecules was observed.
  
  
  CONCLUSIONS
  Seizure-like activity in an in vitro isolated brain preparation induces the expression of adhesion molecules in the cerebral endothelium. These observations indicate that local endothelial activation may represent a crucial step for the development of an inflammatory response induced by seizures, and suggest a possible novel pathogenic mechanism during the process of epileptogenesis.
authors: Department of Clinical Epileptology and Experimental Neurophysiology, Istituto Nazionale Neurologico, Milan, Italy.
authors: Laura
authors: L
authors: Librizzi
authors: Maria Cristina
authors: MC
authors: Regondi
authors: Chiara
authors: C
authors: Pastori
authors: Simona
authors: S
authors: Frigerio
authors: Carolina
authors: C
authors: Frassoni
authors: Marco
authors: M
authors: de Curtis
mesh_terms: D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D001921:Brain / Q000098:blood supply / Q000187:drug effects / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000378:metabolism*; D004594:Electrophysiology; D004660:Encephalitis / Q000378:metabolism; D004730:Endothelium, Vascular / Q000737:chemistry / Q000378:metabolism*; D004827:Epilepsy / Q000139:chemically induced / Q000378:metabolism*; D006168:Guinea Pigs; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000378:metabolism; D008032:Limbic System / Q000187:drug effects / Q000378:metabolism; D019579:Neocortex / Q000187:drug effects / Q000378:metabolism; D009474:Neurons / Q000737:chemistry / Q000378:metabolism; D019007:P-Selectin / Q000032:analysis / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis / Q000378:metabolism; D014018:Tissue Distribution; D015854:Up-Regulation
chemical_list: D015815:Cell Adhesion Molecules; D019007:P-Selectin; D016760:Proto-Oncogene Proteins c-fos; D018799:Intercellular Adhesion Molecule-1; D001640:Bicuculline