title: Evaluation of antioxidant effect of extracts of Symphyopappus casarettoi.
abstract: The ethanolic extract of Symphyopappus casarettoi syn. Eupatorium casarettoi was partionated in hexanic, chloroformic and methanolic fractions. Extract and fractions were tested for their antioxidant activity in vitro and ex vivo assays. The methanolic fraction showed a higher antioxidant potential compared to the others fractions, which was correlated with its total phenol content. In addition, the ethanolic extract and the methanolic fraction attenuated ex vivo iron-induced cell death, quantified by lactate dehydrogenase leakage, and effectively protected against lipid damage induced by iron. These findings suggest that the ethanolic extract of S. casarettoi inflorescence and its methanolic fraction have in vitro and ex vivo antioxidant properties.
authors: Curso de Pós-Graduação em Ciências Biológicas-Bioquímica, Instituto de Ciências Básicas de Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600 (anexo), Porto Alegre, RS, CEP: 90035-003, Brazil. marare1@hotmail.com
authors: Mara Regina
authors: MR
authors: Netto Benetti
authors: Martina
authors: M
authors: Rudnicki
authors: Alfeu
authors: A
authors: Zanotto-Filho
authors: Marcos Roberto
authors: MR
authors: de Oliveira
authors: Andréa Gisiane
authors: AG
authors: Kurek
authors: Adriana
authors: A
authors: Coitinho
authors: Gilberto
authors: G
authors: Schwartsmann
authors: José Cláudio Fonseca
authors: JC
authors: Moreira
mesh_terms: D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects; D019659:Asteraceae; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007501:Iron; D015227:Lipid Peroxidation / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D017392:Thiobarbituric Acid Reactive Substances / Q000737:chemistry
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; D007501:Iron