title: The gene expression profiling in murine cortical cells undergoing programmed cell death (PCD) induced by serum deprivation.
abstract: PCD (programmed cell death) is important mechanism for development, homeostasis and disease. To analyze the gene expression pattern in brain cells undergoing PCD in response to serum deprivation, we analyzed the cDNA microarray consisting of 2,300 genes and 7 housekeeping genes of cortical cells derived from mouse embryonic brain. Cortical cells were induced apoptosis by serum deprivation for 8 hours. We identified 69 up-regulated genes and 21 down-regulated genes in apoptotic cells. Based on the cDNA microarray data four genes were selected and analyzed by RT-PCR and northern blotting. To characterize the role of UNC-51-like kinase (ULK2) gene in PCD, we investigated cell death effect by ULK2. And we examined expression of several genes that related with PCD. Especially GAPDH was increased by ULK2. Theses findings indicated that ULK2 is involved in apoptosis through p53 pathway.
authors: Department of Biological Science, Sookmyung Womenos University, Seoul 140-742, Korea.
authors: Moon Hee
authors: MH
authors: Yang
authors: Kyung Hyun
authors: KH
authors: Yoo
authors: Yeon Joo
authors: YJ
authors: Yook
authors: Eun Young
authors: EY
authors: Park
authors: Jeong Ok
authors: JO
authors: Jeon
authors: Seo Hee
authors: SH
authors: Choi
authors: So Young
authors: SY
authors: Park
authors: Yu Mi
authors: YM
authors: Woo
authors: Min Joo
authors: MJ
authors: Lee
authors: Jong Hoon
authors: JH
authors: Park
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects; D020869:Gene Expression Profiling; D051379:Mice; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D044967:Serum / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D012333:RNA, Messenger; C401061:Ulk2 protein, mouse; D017346:Protein Serine-Threonine Kinases