Tyrosine sulfation of statherin.
Tyrosylprotein sulfotransferase (TPST), responsible for the sulfation of a variety of secretory and membrane proteins, has been identified and characterized in submandibular salivary glands (William et al. Arch Biochem Biophys 1997; 338: 90-96). In the present study we demonstrate the sulfation of a salivary secretory protein, statherin, by the tyrosylprotein sulfotransferase present in human saliva. Optimum statherin sulfation was observed at pH 6.5 and at 20 mm MnCl(2). Increase in the level of total sulfation was observed with increasing statherin concentration. The K(m)value of tyrosylprotein sulfotransferase for statherin was 40 microM. Analysis of the sulfated statherin product on SDS-polyacrylamide gel electrophoresis followed by autoradiography revealed (35)S-labelling of a 5 kDa statherin. Further analysis of the sulfated statherin revealed the sulfation on tyrosyl residue. This study is the first report demonstrating tyrosine sulfation of a salivary secretory protein. The implications of this sulfation of statherin in hydroxyapatite binding and Actinomyces viscosus interactions are discussed.
Kasinathan
C
C
Oral Biology, NJ Dental School, University of Medicine and Dentistry of New Jersey, Newark, NJ 07103-2400, USA.
Gandhi
N
N
Ramaprasad
P
P
Sundaram
P
P
Ramasubbu
N
N
D016924:Actinomyces viscosus / Q000502:physiology; D000328:Adult; D001422:Bacterial Adhesion; D002712:Chlorides / Q000378:metabolism; D017886:Durapatite / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D017895:Manganese Compounds / Q000378:metabolism; D011485:Protein Binding; D011499:Protein Processing, Post-Translational; D012463:Saliva / Q000201:enzymology*; D012471:Salivary Proteins and Peptides / Q000737:chemistry / Q000378:metabolism*; D015238:Sulfotransferases / Q000302:isolation & purification / Q000378:metabolism*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism
D002712:Chlorides; D017895:Manganese Compounds; C018149:STATH protein, human; D012471:Salivary Proteins and Peptides; C005662:tyrosine O-sulfate; D014443:Tyrosine; D017886:Durapatite; D015238:Sulfotransferases; C039079:protein-tyrosine sulfotransferase; C025340:manganese chloride