Long-lasting anti-inflammatory properties of Crotalus durissus terrificus snake venom in mice.
In the present study, we investigated the effects of Crotalus durissus terrificus venom (CdtV) on vascular and cellular events of inflammation induced by carrageenan (cg) in mice. To evaluate edema, CdtV (75 microg kg(-1)) was administered subcutaneously before (1h, 7 or 14 days) or after (1, 4 or 48 h) subplantar injection of cg (15 mg kg(-1)) into the mouse right hind paw; to analyze leukocyte influx, cg (200 microL) was injected i.p. in mice. The inhibitory action of CdtV on edema, either before or after cg injection, was prolonged, lasting even 72 h after administration. Besides, CdtV significantly inhibited migration of polymorphonuclear cells to peritoneal cavity when administered before or after cg. Such inhibitory effects of CdtV on edema and cell migration were also compared with well-known anti-inflammatory drugs. The results demonstrated that CdtV, when injected either 7 or 14 days or 1h before cg, induced a more effective and long-lasting anti-inflammatory effect than that observed with classical anti-inflammatory drugs. The association of CdtV with different drugs did not potentialize their actions on cell migration. These results demonstrate that CdtV exhibits long-lasting anti-inflammatory effects.
Nunes
Fernanda P B
FP
Laboratory of Pathophysiology, Butantan Institute, AV. Vital Brazil, 1500, 05503-900, São Paulo, Brazil.
Sampaio
Sandra C
SC
Santoro
Marcelo L
ML
Sousa-e-Silva
Maria Cristina C
MC
D000704:Analysis of Variance; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002351:Carrageenan / Q000633:toxicity; D002465:Cell Movement / Q000187:drug effects; D003435:Crotalid Venoms / Q000494:pharmacology / Q000627:therapeutic use*; D017839:Crotalus; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy*; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy*; D051379:Mice; D009504:Neutrophils / Q000187:drug effects
D000893:Anti-Inflammatory Agents; D003435:Crotalid Venoms; D002351:Carrageenan