Sugarcane borer (Lepidoptera: Crambidae) resistance to transgenic Bacillus thuringiensis maize.
Transgenic maize, Zea mays L., expressing the Bacillus thuringiensis (Bt) CrylAb toxin has been planted to extensive areas across the United States and several other countries, but no resistance has been documented in field populations oflepidopteran target pests. This article describes the first report of resistance alleles to commercially available Cry1Ab Bt maize in a Louisiana population of sugarcane borer, Diatraea saccharalis (F.) (Lepidoptera: Crambidae). Two hundred thirteen two-parent isolines of D. saccharalis were screened for Cry1Ab resistance on Bt maize leaf tissue using an F2 screening technique. Larvae representing three isolines survived >15 d on Bt tissue in the F2 generation. The second generation backcross progeny (B1F2) derived from isoline 52 completed larval development on Bt maize in the greenhouse. Segregation and resistance frequency analysis associated with isoline 52 suggested that Bt resistance is probably determined by a nearly completely recessive allele at a single locus. With this assumption, the estimated resistance allele frequency in this population is 0.0023, within a 95% confidence interval of 0.0003-0.0064.
Huang
Fangneng
F
Department of Entomology, Louisiana State University AgCenter, Baton Rouge, LA 70803, USA. fhuang@agcenter.lsu.edu
Leonard
B Rogers
BR
Andow
David A
DA
D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology*; D001427:Bacterial Toxins / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology*; D004731:Endotoxins / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant; D006460:Hemolysin Proteins / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology*; D007305:Insecticide Resistance; D007306:Insecticides / Q000494:pharmacology; D009036:Moths / Q000187:drug effects* / Q000235:genetics; D030821:Plants, Genetically Modified; D003313:Zea mays / Q000235:genetics*
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis