[Genes of insecticidal crystal proteins with dual specificity in Bacillus thuringiensis strains, isolated in the Crimea territory].
The insecticidal crystal proteins of 15 B. thuringiensis strains, isolated in the Crimea territory that are toxical for some Lepidoptera and Colorado potato beetle larvae were identified by PAGE electrophoresis. Ten strains produced the crystal proteins with high molecular weight (> 120 kD). PCR with use of broad specificity primers and DNA of these B. thuringiensis strains as template demonstrated the specific PCR products (1000 bp). Amplified DNA fragments were cloned and sequenced. The nucleotide sequence analysis revealed that the genomes of ten strains of B. thuringiensis carried Cry1B genes, which are responsible for production of the insecticidal crystal proteins with dual specificity. The influence of the solubilization conditions on the structure and toxicity of Cry1B protein for Colorado potato beetle larvae was shown. The dual toxicity of studied B. thuringiensis strains is explained by the Cry1B genes presence in their genomes. These strains may be used to develop the broad specificity bioinsecticides.
Rymar
S Iu
SIu
Isakova
I A
IA
Kuznietsova
L M
LM
Kordium
V A
VA
D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics* / Q000302:isolation & purification; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics / Q000494:pharmacology / Q000502:physiology; D001427:Bacterial Toxins / Q000235:genetics / Q000494:pharmacology; D001517:Coleoptera / Q000187:drug effects / Q000254:growth & development; D004269:DNA, Bacterial; D004591:Electrophoresis, Polyacrylamide Gel; D004731:Endotoxins / Q000235:genetics / Q000494:pharmacology / Q000502:physiology; D005798:Genes, Bacterial; D006460:Hemolysin Proteins / Q000235:genetics / Q000494:pharmacology / Q000502:physiology; D007814:Larva / Q000187:drug effects; D010957:Plasmids; D012988:Soil Microbiology; D014455:Ukraine
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis