The enhanced diffusional mixing for latex immunoagglutination assay in a microfluidic device.
Latex immunoagglutination assay in a microfluidic device is expected to be even easier than its large-sized, commercialized counterpart. However, such demonstration has had a limited success due to the difficulties in mixing in a microfluidic device, especially for the microparticles used in latex immunoagglutination assay. The primary goal of this work is to improve diffusional mixing towards the successful latex immunoagglutination in a microfluidic devices without any non-specific binding. To this end, SDS (sodium dodecyl sulfate, an ionic surfactant) or Tween 80 (polyethylene sorbitol ester, a non-ionic surfactant) was added to the antibody-conjugated polystyrene (PS) microparticle suspension. These surfactant-added particle suspensions were mixed with the target antigen solution at the Y-junction of a microfluidic device. The immunoagglutination and the diffusion behavior were visually identified with an inverted light microscope. Both surfactants showed some problems such as non-specific binding (with SDS) or very poor diffusion (with Tween 80). As an alternative approach, therefore, highly carboxylated PS microparticles, where the surface is saturated with carboxyl-terminated side chains, were evaluated without using any surfactants. These particles showed very low non-specific binding comparable to that with Tween 80 and good diffusional mixing equivalent to that with SDS.
Han
Jin-Hee
JH
Department of Agricultural and Biosystems Engineering, The University of Arizona, Tucson, AZ 85721-0038, USA.
Kim
Kye-Seong
KS
Yoon
Jeong-Yeol
JY
D000941:Antigens / Q000737:chemistry / Q000276:immunology; D004058:Diffusion; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004784:Environmental Monitoring / Q000295:instrumentation; D004867:Equipment Design; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology; D007841:Latex Fixation Tests / Q000295:instrumentation; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D011136:Polysorbates / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D012828:Silicones / Q000737:chemistry; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry
D000941:Antigens; D004129:Dimethylpolysiloxanes; D007074:Immunoglobulin G; D011136:Polysorbates; D011137:Polystyrenes; D012828:Silicones; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; C013830:baysilon