Toll-like receptor 3 agonist enhances IFN-gamma and TNF-alpha production by murine uterine NK cells.
To understand the response of murine uterine natural killer (uNK) cells to Toll-like receptor (TLR) 3 agonist at the early gestation stage, CBA x DBA/2 mice were intraperitoneally (i.p.) injected with polyinosinic-polycytidylic acid (poly I:C), the specific TLR3 agonist, at a dose of 10 microg/g BW or PBS at gestation day (gd) 6.5. The CD69 expression of uNK (DX5(+)CD3(-)) cells was highly up-regulated and reached 92.3+/-0.9%, the percentage of intracellular TNF-alpha(+) or IFN-gamma(+) uNK (DX5(+)CD3(-)) cells in the implantation sites of CBAxDBA/2 matings was also significantly increased 24 h after poly I:C injection. Surprisingly, poly I:C treatment significantly decreased the total number of uNK cells (either DX5(+)CD3(-) or DBA(+)) at fetal-maternal surface, but had no influence on local NKT cells, T cells and DCs. This investigation will help to explain the central role for hyperactivated uNK cells in the progress of mice pregnancy.
Zhang
Jianhong
J
Institute of Immunology, Hefei National Laboratory for Physical Sciences at Microscale, China; School of Life Sciences, University of Science and Technology of China, 443 Huangshan Road, Hefei, Anhui 230027, China.
Sun
Rui
R
Wei
Haiming
H
Wu
Dongmei
D
Tian
Zhigang
Z
D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis; D039521:CD11c Antigen / Q000276:immunology; D002469:Cell Separation; D005260:Female; D005455:Fluorescent Antibody Technique; D007369:Interferon Inducers / Q000494:pharmacology; D007371:Interferon-gamma / Q000096:biosynthesis*; D007694:Killer Cells, Natural / Q000378:metabolism*; D037181:Lectins, C-Type; D051379:Mice; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D011070:Poly I-C / Q000494:pharmacology; D011247:Pregnancy; D051196:Toll-Like Receptor 3 / Q000819:agonists*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*; D014599:Uterus / Q000166:cytology / Q000378:metabolism*; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D039521:CD11c Antigen; D007369:Interferon Inducers; D007371:Interferon-gamma; D037181:Lectins, C-Type; D011070:Poly I-C; D051196:Toll-Like Receptor 3; D014409:Tumor Necrosis Factor-alpha; D000099192:CD69 Antigens