Blood glucose monitor: an alternative off-line method to measure glucose concentration during fermentations with Trichoderma reesei.
Two home, blood-glucose monitoring meters, OneTouch Ultra and Ascensia Contour, were used to determine the glucose concentration during fermentations of Trichoderma reesei in both flasks and bioreactors. The results, when compared to those given by the 3,5-dinitrosalicylic acid reducing sugar assay, HPLC and YSI 2700 SELECT Biochemistry analyzer, showed that the glucose meters are a quick, reliable and economical alternative method for frequent glucose concentration measurement during fermentation. For T. reesei fermentations, the OneTouch meter was the more suitable.
Choy
Viviane
V
Department of Chemical Engineering, University of Ottawa, Ottawa, Ontorio, Canada.
Patel
Nilesh
N
Thibault
Jules
J
D018533:Biomass; D019149:Bioreactors; D015190:Blood Glucose Self-Monitoring / Q000295:instrumentation* / Q000379:methods*; D005285:Fermentation; D005947:Glucose / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D012044:Regression Analysis; D012996:Solutions; D014242:Trichoderma / Q000378:metabolism*
D012996:Solutions; D005947:Glucose