title: Site-specific effect of estradiol on gene expression in the adipose tissue of ob/ob mice.
abstract: Sex hormones are likely to be involved in sex differences in adipose tissue distribution. To test whether estrogen regulates genes expressed in the adipose tissue in a site-specific manner, we studied the effect of exogenous estradiol on the gene expression in visceral and subcutaneous adipose tissues of male ob/ob mice. We screened genes expressed in a site- and sex-specific manner, and genes that were affected by exogenous estradiol by DNA chip analysis. They were verified by real-time PCR. Myosin heavy chain 2B (Myh4) and phosphoglycerate mutase muscle-specific subunit (Pgam) were expressed specifically in the subcutaneous adipose tissue, and uroplakin IIIb (UP3) was expressed specifically in the visceral adipose tissue. DEAD-box Y RNA helicase (DBY) and eukaryotic initiation factor 2 gamma Y (eIF2gamma Y) were expressed only in male adipose tissue. X-chromosome inactive specific transcript (Xist) was expressed only in female adipose tissue. When estradiol was subcutaneously administrated to male mice, the expression of monocyte-chemoattractant protein-1 (MCP-1) and androgen receptor (AR) genes was regulated in a site-specific manner. The difference in the amount of estrogen receptor did not account for the site-specific effect of estrogen. Our findings show that estrogen affects the expression of some adipocyte genes in a site-specific manner.
authors: Geriatrics and Vascular Medicine, Tokyo Medical and Dental University Graduate School, Yushima Bunkyo-ku, Tokyo, Japan.
authors: S
authors: S
authors: Shinozaki
authors: T
authors: T
authors: Chiba
authors: K
authors: K
authors: Kokame
authors: T
authors: T
authors: Miyata
authors: M
authors: M
authors: Ai
authors: A
authors: A
authors: Kawakami
authors: E
authors: E
authors: Kaneko
authors: M
authors: M
authors: Yoshida
authors: K
authors: K
authors: Shimokado
mesh_terms: D000818:Animals; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D050152:Intra-Abdominal Fat / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D051379:Mice; D008820:Mice, Obese; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism; D012727:Sex Characteristics; D050151:Subcutaneous Fat / Q000187:drug effects* / Q000378:metabolism*
chemical_list: C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D012333:RNA, Messenger; D011944:Receptors, Androgen; D011960:Receptors, Estrogen; D004958:Estradiol