Alterations in the molecular species of plasmalogen phospholipids and glycolipids due to peroxisomal dysfunction in Chinese hamster ovary-mutant Z65 cells by FABMS method.
Changes in the molecular species of lipids associated with Pex2 gene-mutation were investigated to elucidate the pathogeneses of peroxisome biogenesis disorders. Although no differences were observed in the concentrations of cholesterol and phosphatidyl choline between mutated Z65 and control CHO-K1 cells, the amounts of cholesterol esters and glycolipids in Z65 cells were twice those in CHO-K1 cells, but phosphatidyl ethanolamine (PE), particularly 1-O-octadec-1'-enyl-2-oleoyl PE, was absent in Z65 cells by FABMS. Enhanced synthesis of glycolipids in Z65 cells was associated with an abundance of lignoceric acid-containing ones, suggesting a role of glycolipids in the retention of longer saturated fatty acids.
Saito
Makiko
M
Department of Pediatrics, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
Horikawa
Makoto
M
Iwamori
Yuriko
Y
Sakakihara
Yoichi
Y
Mizuguchi
Masashi
M
Igarashi
Takashi
T
Fujiki
Yukio
Y
Iwamori
Masao
M
D000818:Animals; D016466:CHO Cells; D002855:Chromatography, Thin Layer; D006224:Cricetinae; D003412:Cricetulus; D006017:Glycolipids / Q000145:classification / Q000378:metabolism*; D020675:Peroxisomes / Q000502:physiology*; D010955:Plasmalogens / Q000145:classification / Q000378:metabolism*; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000379:methods*
D006017:Glycolipids; D010955:Plasmalogens