Characterizing assembly morphology changes during solubilization process of dimyristoyl phosphocholine vesicles by n-dodecyl triethylammonium bromide.
In the present work, the assembly morphology changes during the solubilization process of the sonicated unilamellar vesicles from dimyristoyl phosphocholine (DMPC) by a cationic surfactant, n-dodecyl triethylammonium bromide (DTEAB) were well characterized with DSC, FF-TEM and DLS and fluorescence probes technique. Based on an analysis on the above results, a primary multi-stage model was brought forward to sketch the assembly morphology changes during the DMPC vesicle solubilization by DTEAB. In comparison with classical models, vesicles division, tubule-like structure formation and fission to vesicle were found in the middle stages of this model. Additionally, it is the first time that the transversally-cut profiles of tubule-like structures were observed during vesicle solubilization process.
Wang
Shaoqing
S
State Key Laboratory for Structural Chemistry of Unstable and Stable Species, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, People's Republic of China.
Huang
Jianbin
J
Song
Qian
Q
Fu
Honglan
H
D002152:Calorimetry, Differential Scanning; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D004161:Diphenylhexatriene / Q000737:chemistry; D005453:Fluorescence; D008027:Light; D046529:Microscopy, Electron, Transmission / Q000379:methods; D010316:Particle Size; D011721:Pyrenes / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D012995:Solubility; D013499:Surface Properties
D011721:Pyrenes; D000644:Quaternary Ammonium Compounds; D004161:Diphenylhexatriene; C030984:pyrene; C022806:dodecyltrimethylammonium; D004134:Dimyristoylphosphatidylcholine