N,N'-Bis-(2-(cyano)ethoxycarbonyl)-2-methyl-2-thiopseudourea: a guanylating reagent for synthesis of 2'-O-[2-(Guanidinium)ethyl]-modified oligonucleotides.
A guanylating reagent, N,N'-bis-(2-(cyano)ethoxycarbonyl)-2-thiopseudourea, was synthesized and used for synthesis of 2'-O-[2-(guanidinium)ethyl] (2'-O-GE) modified oligonucleotides. A convenient deprotection method for the 2'-O-GE oligonucleotides was developed.
Prakash
Thazha P
TP
Department of Medicinal Chemistry, Isis Pharmaceuticals Inc., Carlsbad, California 92008. USA.
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Manoharan
Muthiah
M
D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D009841:Oligonucleotides / Q000138:chemical synthesis*; D013890:Thiourea / Q000031:analogs & derivatives* / Q000737:chemistry
C519049:N,N'-bis-(2-(cyano)ethoxycarbonyl)-2-methyl-2-thiopseudourea; D009841:Oligonucleotides; D013890:Thiourea