title: Female-specific DNA sequences in the chicken genome.
abstract: Eight in silico W-specific sequences from the WASHUC1 chicken genome assembly gave female-specific PCR products using chicken DNA. Some of these fragments gave female-specific products with turkey and peacock DNA. Sequence analysis of these 8 fragments (3077 bp total) failed to detect any polymorphisms among 10 divergent chickens. In contrast, comparison of the DNA sequences of chicken with those of turkey and peacock revealed a nucleotide difference every 25 and 28 bp, respectively. Radiation hybrid mapping verified that these amplicons exist only on chromosome W. The homology of 6 W-specific fragments with chromo-helicase-DNA-binding gene and expressed sequenced tags from chicken and other species indicate that these fragments may have or have had a biological function. These fragments may be used for early sexing in commercial chicken and turkey flocks.
authors: Robert H. Smith Institute of Plant Sciences and Genetics, The Hebrew University of Jerusalem, Rehovot 76100, Israel.
authors: Zur
authors: Z
authors: Granevitze
authors: Shula
authors: S
authors: Blum
authors: Hans
authors: H
authors: Cheng
authors: Alain
authors: A
authors: Vignal
authors: Mireille
authors: M
authors: Morisson
authors: Giora
authors: G
authors: Ben-Ari
authors: Lior
authors: L
authors: David
authors: Marcus William
authors: MW
authors: Feldman
authors: Steffen
authors: S
authors: Weigend
authors: Jossi
authors: J
authors: Hillel
mesh_terms: D000818:Animals; D002645:Chickens / Q000235:genetics*; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics*; D005260:Female; D016678:Genome; D006822:Hybrid Cells; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide
chemical_list: D004247:DNA