title: Presence of virgin olive oil phenolic metabolites in human low density lipoprotein fraction: determination by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry.
abstract: The biological benefits of olive oil in preventing the oxidation of low density lipoprotein (LDL) would seem to be linked to its high monounsaturated fatty acid contents, but also to its respective phenolic compounds contents. One prerequisite to assess the in vivo physiological significance of phenolic compounds is to determine their presence in human LDL following the ingestion of virgin olive oil. In this work, olive oil phenolic metabolites were identified using high-performance liquid chromatography in tandem with electrospray mass spectrometry (HPLC-ESI-MS/MS) detection, after solid phase extraction (SPE). Quantitative methods were developed in carrying out linearity, precision, sensitivity and recovery tests. The results from two methods of LDL separation were compared and shorter LDL isolation procedure showed a better recovery for antioxidants compounds in LDL. The metabolites identified in LDL were: hydroxytyrosol monoglucuronide, hydroxytyrosol monosulfate, tyrosol glucuronide, tyrosol sulfate and homovanillic acid sulfate. The fact that olive oil phenolic metabolites are able to bind LDL strengthens claims that these compounds act as in vivo antioxidants.
authors: Department of Nutrition and Food Science, Reference Center in Food Technology, Faculty of Pharmacy, University of Barcelona, Avda. Joan XXIII s/n, 08028 Barcelona, Spain.
authors: Karina
authors: K
authors: de la Torre-Carbot
authors: Jorge L
authors: JL
authors: Chávez-Servín
authors: Olga
authors: O
authors: Jaúregui
authors: Ana I
authors: AI
authors: Castellote
authors: Rosa M
authors: RM
authors: Lamuela-Raventós
authors: Montserrat
authors: M
authors: Fitó
authors: María-Isabel
authors: MI
authors: Covas
authors: Daniel
authors: D
authors: Muñoz-Aguayo
authors: M Carmen
authors: MC
authors: López-Sabater
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D006801:Humans; D008077:Lipoproteins, LDL / Q000032:analysis* / Q000097:blood; D000069463:Olive Oil; D010636:Phenols / Q000032:analysis / Q000378:metabolism; D010938:Plant Oils / Q000032:analysis* / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D008077:Lipoproteins, LDL; D000069463:Olive Oil; D010636:Phenols; D010938:Plant Oils