title: Mechanical strain stress suppresses expression of HSP70 and wound restoration in gastric mucosal cells.
abstract: The major heat shock protein, HSP70, is known to be involved in cytoprotection against environmental stresses mediated by their function as a "molecular chaperone." However, the influence of HSP70 on gastric mucosal healing under physical stimulation or stress is not completely understood. Rat gastric mucosal cells (RGM-1) were stably transfected with pBK-CMV containing the human HSP70 gene (7018-RGM-1) or pBK-CMV alone (pBK-CMV-12). Artificial wounds were created. Mechanical stretch was applied to 7018-RGM-1 cells or pBK-CMV-12 cells. The effect of mechanical stretch on HSP70 expression was assessed by Western blot analysis. Expression of HSP70 was decreased by mechanical stretch in pBK-CMV-12 cells. However, expression of HSP70 was not decreased by mechanical stretch in 7018-RGM-1 cells. Furthermore, the wound restoration of pBK-CMV-12 cells was suppressed under mechanical stretch condition. On the other hand, the wound restoration of 7018-RGM-1 cells was not affected by mechanical stretch. These results suggest that HSP70 plays an important role in gastric wound healing under physical stress.
authors: Department of Gastroenterology, Akita University School of Medicine, 1-1-1 Hondo, Akita, 010-8543, Japan. odashima@doc.med.akita-u.ac.jp
authors: Masaru
authors: M
authors: Odashima
authors: Michiro
authors: M
authors: Otaka
authors: Tamotsu
authors: T
authors: Matsuhashi
authors: Mario
authors: M
authors: Jin
authors: Youhei
authors: Y
authors: Horikawa
authors: Reina
authors: R
authors: Ohba
authors: Hideaki
authors: H
authors: Itoh
authors: Sumio
authors: S
authors: Watanabe
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D002460:Cell Line; D003587:Cytomegalovirus / Q000235:genetics; D004247:DNA / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D020895:Enterocytes / Q000378:metabolism / Q000473:pathology; D005753:Gastric Mucosa / Q000293:injuries / Q000378:metabolism / Q000473:pathology*; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D051381:Rats; D013314:Stress, Mechanical; D014162:Transfection; D014945:Wound Healing / Q000502:physiology*
chemical_list: D015415:Biomarkers; D018840:HSP70 Heat-Shock Proteins; D004247:DNA