title: Identification of marker genes for intestinal immunomodulating effect of a fructooligosaccharide by DNA microarray analysis.
abstract: Prebiotic fructooligosaccharides are noted for their intestinal immunodulating effects, and the identification of markers for the effects is a matter of great concern. This study aimed to identify marker genes for physiological effects of a particular fructooligosaccharide (FOS) on a host animal and also to define the target of its function in the small intestine. DNA microarray technology was used to screen candidate marker genes, and comprehensive changes in gene expressions in the ileum of mice fed with FOS were investigated. One of the major physiological effects of FOS was intestinal immunomodulation. Marker genes were then identified for major histocompatibility complex classes I and II, interferon, and phosphatidylinositol metabolites. Also, the ileum was segmented into Peyer's patch (PP) and the other ileal organ (DeltaPP), and these were analyzed by quantitative RT-PCR method, with the result that the site for recognizing the FOS function was the DeltaPP rather than the PP. This is the first paper showing the markers for the physiological effects of FOS in the small intestine at gene expression level. Applying these marker genes would make it possible to clarify the mechanisms of how the administration of dietary FOS and associated changes in the intestinal environment are recognized by host organisms as well as how its immunomodulating effects are expressed in the body.
authors: Food and Health R&D Laboratories, Meiji Seika Kaisha, Ltd., 5-3-1 Chiyoda, Sakado-shi, Saitama 350-0289, Japan. tomoyuki_fukasawa@meiji.co.jp
authors: Tomoyuki
authors: T
authors: Fukasawa
authors: Koichiro
authors: K
authors: Murashima
authors: Ichiro
authors: I
authors: Matsumoto
authors: Akira
authors: A
authors: Hosono
authors: Hiroki
authors: H
authors: Ohara
authors: Chuhei
authors: C
authors: Nojiri
authors: Jinnichiro
authors: J
authors: Koga
authors: Hidetoshi
authors: H
authors: Kubota
authors: Minoru
authors: M
authors: Kanegae
authors: Shuichi
authors: S
authors: Kaminogawa
authors: Keiko
authors: K
authors: Abe
authors: Toshiaki
authors: T
authors: Kono
mesh_terms: D000818:Animals; D005260:Female; D005819:Genetic Markers / Q000235:genetics*; D007155:Immunologic Factors / Q000494:pharmacology*; D007422:Intestines / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D020411:Oligonucleotide Array Sequence Analysis; D009844:Oligosaccharides / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D005819:Genetic Markers; D007155:Immunologic Factors; D009844:Oligosaccharides; C116580:fructooligosaccharide