Sequence and organization of the Heliothis virescens ascovirus genome.
The nucleotide sequence of the Heliothis virescens ascovirus (HvAV-3e) DNA genome was determined and characterized in this study. The circular genome consists of 186,262 bp, has a G+C content of 45.8 mol% and encodes 180 potential open reading frames (ORFs). Five unique homologous regions (hrs), 23 'baculovirus repeat ORFs' (bro) and genes encoding a caspase homologue and several enzymes involved in nucleotide replication and metabolism were found in the genome. Several ascovirus (AV)-, iridovirus- and baculovirus-homologous genes were identified. The genome is significantly larger than the recently sequenced genomes of Trichoplusia ni AV (TnAV-2c) and Spodoptera frugiperda AV (SfAV-1a). Gene-parity plots and overall similarity of ORFs indicate that HvAV-3e is related more closely to SfAV-1a than to TnAV-2c.
Asgari
Sassan
S
School of Integrative Biology, University of Queensland, St Lucia, QLD 4072, Australia.
Davis
John
J
Australian Genome Research Facility, University of Queensland, St Lucia, QLD 4072, Australia.
Wood
David
D
Australian Genome Research Facility, University of Queensland, St Lucia, QLD 4072, Australia.
Wilson
Peter
P
Australian Genome Research Facility, University of Queensland, St Lucia, QLD 4072, Australia.
McGrath
Annette
A
Australian Genome Research Facility, University of Queensland, St Lucia, QLD 4072, Australia.
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D029223:Ascoviridae / Q000145:classification / Q000235:genetics*; D016367:Baculoviridae; D001482:Base Composition; D001483:Base Sequence; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005814:Genes, Viral; D016679:Genome, Viral; D017994:Iridovirus / Q000235:genetics; D008969:Molecular Sequence Data; D009036:Moths / Q000821:virology*; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics; D014779:Virus Replication / Q000235:genetics
D004279:DNA, Viral; D014764:Viral Proteins