Comprehensive proteomic analysis of the human amniotic fluid proteome: gestational age-dependent changes.
Amniotic fluid (AF) is a significant contributor to fetal health and constitutes a potential rich source of biomarkers for diagnosis of maternal and fetal disorders. In this study, we performed a comprehensive survey of the proteins expressed in AF, combining gel and liquid-based fractionation approaches coupled with LC-MS/MS analysis. Two-dimensional Liquid Chromatography (2D-LC) analysis identified 118 nonredundant proteins with high confidence. One- and two-dimensional gel electrophoresis and in-gel digestion identified 101 proteins. Combining both sets resulted in 219 proteins, of which 96 are unique to AF; 70, 18, and 35 proteins are present in serum, cervico-vaginal fluid, and all three fluids, respectively. Fluorescence two-dimensional differential in-gel electrophoresis (2D-DIGE) comparison of first-, second-, and third-trimester AF samples revealed that maximal differences in the relative abundance of AF proteins occur between the first and second trimesters. A systematic analysis of proteins present both in AF and maternal serum could lead to the development of new noninvasive diagnostic procedures to monitor fetal status.
Michaels
John-Edward A
JE
ProteoGenix, Inc., Portland, Oregon 97213, USA.
Dasari
Surendra
S
Pereira
Leonardo
L
Reddy
Ashok P
AP
Lapidus
Jodi A
JA
Lu
Xinfang
X
Jacob
Thomas
T
Thomas
Archana
A
Rodland
Matthew
M
Roberts
Charles T
CT
Gravett
Michael G
MG
Nagalla
Srinivasa R
SR
D000367:Age Factors; D000653:Amniotic Fluid / Q000737:chemistry*; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D007109:Immunity; D011247:Pregnancy; D021381:Protein Transport; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D044967:Serum / Q000737:chemistry; D015398:Signal Transduction
D020543:Proteome