[Promoting effect of liver regeneration in hepatocarcinogenesis initiated by diethylnitrosamine in the rat].
Preneopliastic cells initiated by a subcarcinogenetic diethylnitrosamine administration must be submitted to subsequent promoting treatments to reach malignancy. In order to elucidate this promotion mechanism we compare the cell kinetics regeneration induced either by a two-thirds hepatectomy or by two successive one-third hepatectomies. The two-thirds hepatectomy triggers acute well marked circadian mitotic waves of high amplitude. But, during this regeneration the preneoplastic cells remain without any proliferative advantage and without promoting effect. The first one-third hepetectomy induces two mitotic circadian waves of low amplitude; the second one-third hepatectomy recrutes 92% of cells already dividing after the first operation. These two successive regenerations liberate an important selective growth of preneoplastic cells and the tumor incidence reaches 96%. These results suggest that preneoplastic cells are selectively promoted by successive mitotic cycles induced by low mitogenic stimulations instead of by an acute stimulation.
Barbason
H
H
Service de cancérologie expérimentale, université de Liège, 4877 Olne, Belgique. HB531748@euphonynet.be
D000818:Animals; D004052:Diethylnitrosamine / Q000633:toxicity*; D006498:Hepatectomy; D008099:Liver / Q000473:pathology; D008113:Liver Neoplasms / Q000139:chemically induced* / Q000473:pathology / Q000503:physiopathology; D008115:Liver Regeneration / Q000187:drug effects; D008940:Mitotic Index; D051381:Rats
D004052:Diethylnitrosamine