Immobilized polysaccharide derivatives: chiral packing materials for efficient HPLC resolution.
Polysaccharide-based chiral packing materials (CPMs) for high-performance liquid chromatography have frequently been used not only to determine the enantiomeric excess of chiral compounds but also to preparatively resolve a wide range of racemates. However, these CPMs can be used with only a limited number of solvents as mobile phases because some organic solvents, such as tetrahydrofuran, chloroform, and so on, dissolve or swell the polysaccharide derivatives coated on a support, e.g., silica gel, and destroy their packed columns. The limitation of mobile phase selection is sometimes a serious problem for the efficient analytical and preparative resolution of enantiomers. This defect can be resolved by the immobilization of the polysaccharide derivatives onto silica gel. Efficient immobilizations have been attained through the radical copolymerization of the polysaccharide derivatives bearing small amounts of polymerizable residues and also through the polycondensation of the polysaccharide derivatives containing a few percent of 3-(triethoxysilyl)propyl residue.
Ikai
Tomoyuki
T
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan.
Yamamoto
Chiyo
C
Kamigaito
Masami
M
Okamoto
Yoshio
Y
D000688:Amylose / Q000031:analogs & derivatives* / Q000737:chemistry*; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D003432:Cross-Linking Reagents / Q000737:chemistry; D017953:Isocyanates / Q000737:chemistry; D015394:Molecular Structure; D058428:Silica Gel; D012822:Silicon Dioxide / Q000737:chemistry; D013237:Stereoisomerism; D014753:Vinyl Compounds / Q000737:chemistry
D003432:Cross-Linking Reagents; D017953:Isocyanates; D014753:Vinyl Compounds; D058428:Silica Gel; D012822:Silicon Dioxide; D002482:Cellulose; D000688:Amylose