Overexpression of 14-3-3gamma causes polyploidization in H322 lung cancer cells.
The 14-3-3 proteins are a family of highly conserved proteins that participate in a wide variety of cellular processes. Mounting evidence suggests that 14-3-3 proteins have a role in human cancers, however their role in tumorigenesis is unclear. Here we report that over-expression of 14-3-3 gamma protein in human lung cancer cell line H322 results in abnormal DNA replication and polyploidization. Cells that overexpress 14-3-3 gamma are resistant to microtubule inhibitors and can reenter the cell cycle in the absence of mitosis suggesting that elevated levels of 14-3-3 gamma may enable cells to bypass the mitotic checkpoint. Taken together, our data indicate that 14-3-3gamma may contribute to tumorigenesis by promoting genomic instability.
Qi
Wenqing
W
Arizona Cancer Center, Department of Cell Biology and Anatomy, 1515 N. Campbell Ave., University of Arizona, Tucson, Arizona 85724, USA.
Liu
Xiaobing
X
Chen
Weiming
W
Li
Qiang
Q
Martinez
Jesse D
JD
D048948:14-3-3 Proteins / Q000235:genetics*; D002453:Cell Cycle; D004261:DNA Replication; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D008940:Mitotic Index; D015739:Nocodazole / Q000494:pharmacology; D011123:Polyploidy; D011494:Protein Kinases / Q000032:analysis; D050257:Tubulin Modulators / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D048948:14-3-3 Proteins; D050257:Tubulin Modulators; D011494:Protein Kinases; C102333:histone H1 kinase; D015739:Nocodazole