title: Analytical method for localizing a fluorescent inclusion in a turbid medium.
abstract: We describe a novel method for localizing a fluorescent inclusion in a homogeneous turbid medium through the use of time-resolved techniques. Based on the calculation of the mean time of the fluorescence curves, the method does not require a priori knowledge of either the fluorescence lifetime or the mean time of the instrument response function since it adopts a differential processing approach. Theoretical expressions were validated and experiments for assessing the accuracy of localization were carried out on liquid optical phantoms with a small fluorescent inclusion. The illumination and detection optical fibers were immersed in the medium to achieve infinite medium geometry as required by the model used. The experimental setup consisted of a time-correlated single-photon counting system. Submillimeter accuracy was achieved for the localization of the inclusion.
authors: Département micro Technologies pour la Biologie et la Santé, CEA-LETI Recherche Technologique Grenoble, Grenoble, France. aurelie.laidevant@wanadoo.fr
authors: A
authors: A
authors: Laidevant
authors: A
authors: A
authors: Da Silva
authors: M
authors: M
authors: Berger
authors: J
authors: J
authors: Boutet
authors: J-M
authors: JM
authors: Dinten
authors: A C
authors: AC
authors: Boccara
mesh_terms: D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D053758:Nanoparticles / Q000648:ultrastructure*; D009391:Nephelometry and Turbidimetry / Q000295:instrumentation* / Q000379:methods; D010316:Particle Size; D019047:Phantoms, Imaging