Adherence to HeLa cells, typing by killer toxins and susceptibility to antifungal agents of Candida dubliniensis strains.
The aim of this study was to evaluate the adherence capability to HeLa cells, the susceptibility to killer toxins and the in vitro susceptibility to antifungal agents (eTest method--AB Biodisk, Solna, Sweden) of 9 Candida dubliniensis isolates recovered from HIV+ and AIDS patients. The adherence test was strongly positive for strain ATCC 777 and positive for all other strains. Typing by killer toxins revealed two different biotypes among the 9 isolates studied: 888 and 688. Only biotype 688 (ATCC 777) was susceptible to the K2 toxin. There was a significant inverse correlation between adherence and killer toxin susceptibility (r = -0.8525 - p = 0.0035). No strains presented resistance to fluconazole, itraconazole, ketoconazole, voriconazole, flucytosine or amphotericin-B. With the exception of ATCC 777, all the other isolates presented similar behavior.
Silva
Gismari Miranda da
GM
Department of Stomatology, School of Dentistry, University of São Paulo. gismari.miranda@uol.com.br
Silveira
Fernando Ricardo Xavier da
FR
Pires
Maria de Fátima Costa
Mde F
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology; D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000145:classification / Q000187:drug effects* / Q000502:physiology; D002448:Cell Adhesion; D004352:Drug Resistance, Microbial; D005656:Fungal Proteins / Q000032:analysis*; D015658:HIV Infections / Q000382:microbiology; D006367:HeLa Cells / Q000382:microbiology; D006801:Humans; D055646:Killer Factors, Yeast; D008826:Microbial Sensitivity Tests; D016533:Mycological Typing Techniques; D011506:Proteins / Q000032:analysis / Q000494:pharmacology
D000935:Antifungal Agents; D005656:Fungal Proteins; D055646:Killer Factors, Yeast; D011506:Proteins