title: The humoral pattern recognition receptor PTX3 is stored in neutrophil granules and localizes in extracellular traps.
abstract: The long pentraxin (PTX) 3 is produced by macrophages and myeloid dendritic cells in response to Toll-like receptor agonists and represents a nonredundant component of humoral innate immunity against selected pathogens. We report that, unexpectedly, PTX3 is stored in specific granules and undergoes release in response to microbial recognition and inflammatory signals. Released PTX3 can partially localize in neutrophil extracellular traps formed by extruded DNA. Eosinophils and basophils do not contain preformed PTX3. PTX3-deficient neutrophils have defective microbial recognition and phagocytosis, and PTX3 is nonredundant for neutrophil-mediated resistance against Aspergillus fumigatus. Thus, neutrophils serve as a reservoir, ready for rapid release, of the long PTX3, a key component of humoral innate immunity with opsonic activity.
authors: Institut National de la Santé et de la Recherche Médicale, Equipe Avenir, Unité 564, University Hospital of Angers, University of Angers, Angers 49933, France.
authors: Sébastien
authors: S
authors: Jaillon
authors: Giuseppe
authors: G
authors: Peri
authors: Yves
authors: Y
authors: Delneste
authors: Isabelle
authors: I
authors: Frémaux
authors: Andrea
authors: A
authors: Doni
authors: Federica
authors: F
authors: Moalli
authors: Cecilia
authors: C
authors: Garlanda
authors: Luigina
authors: L
authors: Romani
authors: Hugues
authors: H
authors: Gascan
authors: Silvia
authors: S
authors: Bellocchio
authors: Silvia
authors: S
authors: Bozza
authors: Marco A
authors: MA
authors: Cassatella
authors: Pascale
authors: P
authors: Jeannin
authors: Alberto
authors: A
authors: Mantovani
mesh_terms: D000818:Animals; D002097:C-Reactive Protein / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D003594:Cytoplasmic Granules / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism*; D005786:Gene Expression Regulation; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009504:Neutrophils / Q000166:cytology* / Q000378:metabolism; D011485:Protein Binding; D000683:Serum Amyloid P-Component / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D000099153:Pentraxins
chemical_list: D002097:C-Reactive Protein; D000683:Serum Amyloid P-Component; D000099153:Pentraxins