Genetic relatedness of multidrug-resistant Escherichia coli cultured from geographically diverse outpatient, midstream urine specimens.
Genomic DNA from 70 demographically matched geographically diverse pairs of urinary isolates of antimicrobial-susceptible and multidrug-resistant Escherichia coli was restricted using XbaI and analyzed by pulsed-field gel electrophoresis. Antimicrobial-susceptible isolates demonstrated limited genetic relatedness, whereas 2 epidemiologic clusters containing a total of 40 isolates (57.1%) were identified among the multidrug-resistant isolates.
Karlowsky
James A
JA
Department of Clinical Microbiology, Health Sciences Centre/Diagnostic Services of Manitoba, Winnipeg, Manitoba, Canada R3A 1R9. jkarlowsky@hsc.mb.ca
Kasloff
Sam
S
Nichol
Kim A
KA
Hoban
Daryl J
DJ
Zhanel
George G
GG
D000553:Ambulatory Care; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology*; D005260:Female; D005843:Geography; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D017720:Molecular Epidemiology; D012150:Polymorphism, Restriction Fragment Length; D014552:Urinary Tract Infections / Q000453:epidemiology / Q000382:microbiology*; D014556:Urine / Q000382:microbiology
D004269:DNA, Bacterial