Laser-induced photofragmentation of neutral and acidic glycans inside an ion-trap mass spectrometer.
Permethylated acidic and neutral N-glycans representing different types of glycan structures, such as linear and branched sialylated structures, high-mannose type and fucosylated complex type, were photodissociated with 157 nm vacuum ultraviolet light in a linear ion trap. Cross-ring fragments corresponding to high-energy fragmentation pathways were observed in abundance for all studied structures. Some product ions appear diagnostic for a linkage of sialic acid residues and the glycan antenna to which these residues are attached. A conclusive assignment of the fucosylation site of the studied glycan structure has been facilitated through measurement of cross-ring fragmentation resulting from photodissociation.
Devakumar
Arugadoss
A
National Center for Glycomics and Glycoproteomics and Department of Chemistry, Indiana University, 800 E. Kirkwood Avenue, Bloomington, IN 47405, USA.
Mechref
Yehia
Y
Kang
Pilsoo
P
Novotny
Milos V
MV
Reilly
James P
JP
D006801:Humans; D007834:Lasers; D008895:Milk, Human / Q000737:chemistry; D010777:Photochemistry; D011134:Polysaccharides / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry
D011134:Polysaccharides