Le.MAPK and its interacting partner, Le.DRMIP, in fruiting body development in Lentinula edodes.
Development in shiitake mushroom, Lentinula edodes, is a unique process and studies of the molecular basis of this process may lead to improvement in mushroom cultivation. Previous studies have identified a number of signal transduction genes related to mushroom development, but those genes have not been well characterized. The present work characterized a developmentally regulated MAP kinase, Le.MAPK, and its interaction with a novel gene, Le.DRMIP in the signal transduction pathway. The expression profiles of these two genes reveal their importance in fruiting body initiation and development; the Le.DRMIP transcript is localized predominantly in the developing young fruiting body and gills, which further signifies its role in cell differentiation during mushroom development.
Szeto
Carol Y Y
CY
Molecular Biotechnology Programme, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong SAR.
Leung
Grace S
GS
Kwan
H S
HS
D000255:Adenosine Triphosphate / Q000494:pharmacology; D048690:Fruiting Bodies, Fungal / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D020928:Mitogen-Activated Protein Kinases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D021141:Nucleic Acid Amplification Techniques; D011485:Protein Binding / Q000187:drug effects; D034443:RNA Transport / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D020942:Shiitake Mushrooms / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D020798:Two-Hybrid System Techniques; D001616:beta-Galactosidase / Q000378:metabolism
D005656:Fungal Proteins; D012333:RNA, Messenger; D000255:Adenosine Triphosphate; D020928:Mitogen-Activated Protein Kinases; D001616:beta-Galactosidase