title: Controlled release of platelet growth factors enhances bone regeneration at rabbit calvaria.
abstract: OBJECTIVE
  Platelet-rich plasma (PRP) has been clinically employed to promote bone regeneration. However, few studies have investigated the enhancement of biological function of platelet growth factors after integration of PRP into biomaterials. In this study, the feasibility of gelatin hydrogels for controlled release of platelet growth factors and the consequent enhancement of PRP-induced bone regeneration were evaluated in rabbit calvarial defect.
  
  
  METHODS
  Gelatin hydrogels incorporating PRP, PRP-activated thrombin, or an empty gelatin hydrogel were applied to the defect, or the defect was left untreated. Bone regeneration was evaluated by microfocus computed tomography, peripheral quantitative computed tomography, and histological examinations.
  
  
  RESULTS
  Successful bone regeneration was observed at the bone defect applied with the gelatin hydrogel incorporating PRP, which is in marked contrast to other groups.
  
  
  CONCLUSIONS
  The gelatin hydrogel is a promising material capable of controlled release of platelet growth factors to enhance bone regeneration.
authors: Department of Biomaterials, Field of Tissue Engineering, Institute for Frontier Medical Sciences, Kyoto University, Kawara-cho Shogoin, Sakyo-ku, Kyoto, Japan.
authors: Akishige
authors: A
authors: Hokugo
authors: Yasunori
authors: Y
authors: Sawada
authors: Rie
authors: R
authors: Hokugo
authors: Hiromitsu
authors: H
authors: Iwamura
authors: Masakiyo
authors: M
authors: Kobuchi
authors: Toshiyuki
authors: T
authors: Kambara
authors: Shosuke
authors: S
authors: Morita
authors: Yasuhiko
authors: Y
authors: Tabata
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use*; D001861:Bone Regeneration / Q000187:drug effects*; D003692:Delayed-Action Preparations; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000627:therapeutic use*; D036341:Intercellular Signaling Peptides and Proteins / Q000008:administration & dosage*; D053657:Platelet-Rich Plasma; D011817:Rabbits; D012886:Skull / Q000601:surgery; D014057:Tomography, X-Ray Computed
chemical_list: D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D036341:Intercellular Signaling Peptides and Proteins; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate