title: Fluorescence detection with high time resolution: from optical microscopy to simultaneous force and fluorescence spectroscopy.
abstract: Picosecond time-resolution fluorescence signal detection over many hours is possible using the time-correlated single photon counting (TCSPC) technique. Advanced TCSPC with clock oscillator set by the pulsed laser and data analysis provides a tool to investigate processes in single molecules on time scale from picoseconds to seconds. Optical imaging techniques combined with TCSPC allow one to study the spatial distribution of fluorescence properties in solution and on a surface. Mechanical manipulation of a single macromolecule by means of an atomic-force microscope makes it possible to detect fluorescence signal changes as a function of mechanical conformations of a fluorescent dye attached to a single DNA molecule.
authors: Institut für Physikalische Chemie, Düsseldorf, Germany. alexander.gaiduk@uni-duesseldorf.de
authors: Alexander
authors: A
authors: Gaiduk
authors: Ralf
authors: R
authors: Kühnemuth
authors: Suren
authors: S
authors: Felekyan
authors: Matthew
authors: M
authors: Antonik
authors: Wolfgang
authors: W
authors: Becker
authors: Volodymyr
authors: V
authors: Kudryavtsev
authors: Carl
authors: C
authors: Sandhagen
authors: Claus A M
authors: CA
authors: Seidel