title: Effects of diet on biomarkers of exposure and effects, and on oxidative damage.
abstract: Modification of DNA is believed to be a key step in carcinogenesis, and therefore DNA adducts have been proposed as predictive biomarkers of human cancer. Smoked and grilled foods are important contributors of PAH-DNA adduct levels, while the consumption of flavonoids and other antioxidants seems to decrease the level of DNA adducts. The aim of this study was to assess the effect of each group of foods and of different dietary profiles on the DNA adducts levels and on oxidative damage to DNA. Occupationally exposed men were recruited in Czech Republic, Slovak Republic and Bulgaria. Non-occupationally exposed subjects were matched on age and gender to PAH-exposed workers. Three hundred and fifty-six subjects who completed the questionnaire for dietary information and had a measurement of DNA adduct levels and oxidative damage to DNA were included in this study. No food item seemed to be individually associated with markers of exposure or DNA damage. Total DNA adducts levels were significantly higher for subjects who had eaten, in the previous 24h, smoked or fried food. A Principal Components Analysis was performed to identify groups of subjects with similar dietary profiles: no significant differences in biomarker levels were observed among the groups defined according to dietary profiles. In conclusion, this study did not show any significant association between diet and biomarkers of DNA damage, oxidative damage to DNA and chromosomal aberrations, neither when each food was considered separately, nor when the effect of different dietary profiles was tested. The recent consumption of smoked or fried food was associated with an increase in total DNA adducts levels.
authors: Fondazione Policlinico, Milano, Italy.
authors: Sara
authors: S
authors: Raimondi
authors: Seymour
authors: S
authors: Garte
authors: Radim J
authors: RJ
authors: Sram
authors: Blanka
authors: B
authors: Binkova
authors: Ivan
authors: I
authors: Kalina
authors: K
authors: K
authors: Lyubomirova
authors: Emanuela
authors: E
authors: Taioli
authors: Rajinder
authors: R
authors: Singh
authors: Peter B
authors: PB
authors: Farmer
mesh_terms: D000393:Air Pollutants / Q000633:toxicity*; D015415:Biomarkers; D002274:Carcinogens, Environmental / Q000633:toxicity*; D018736:DNA Adducts / Q000032:analysis; D004249:DNA Damage; D004032:Diet; D004784:Environmental Monitoring; D006801:Humans; D008297:Male; D016273:Occupational Exposure; D010084:Oxidation-Reduction; D016495:Police; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*; D012907:Smoking
chemical_list: D000393:Air Pollutants; D015415:Biomarkers; D002274:Carcinogens, Environmental; D018736:DNA Adducts; D011084:Polycyclic Aromatic Hydrocarbons