[Characterization of a novel transplantable orthotopic nude mouse model with xenografted human bladder transitional cell tumor (BIU-87)].
OBJECTIVE
A mouse model of orthotopic bladder cancer simulating its human counterpart is of great importance in preclinical evaluation of new treatment modalities such as immunotxin therapy. The aim of the present study is to establish a novel nude mouse model with xenografted human bladder cancer.


METHODS
Single cell suspension of an established human bladder transitional cell carcinoma (TCC) cell line BIU-87 was instilled into nude mouse bladders which were pretreated with mild acid washing. The tumor growth in mouse bladder was assessed weekly by magnetic resonance imaging (MRI). At intervals following implantation and MRI tumor detection, the animals were sacrificed for necropsy, histological examination and immunocytochemical studies.


RESULTS
The overall tumor establishment was 92.9% (52/56 mice) at 7 - 36 days, while in the subgroup of animals sacrificed at 12 - 13 days, 40 out of 42 animals (95.2%) developed TCC, the majority of which was superficial. The tumor stages were assessed by gross and histopathology. Histological examination confirmed the presence of grade II - III TCC. Immunocytochemistry confirmed that the tumor model maintained the biological and immunological features of BIU-87 cells. The changes seen on MRI images well correlated with the extent of tumor invasion identified by histology. Carcinoma in situ could be detected histologically at 7 - 9 days post-inoculation and progressed into papillary or invasive tumors thereafter.


CONCLUSIONS
The orthotopic BIU-87 TCC model in nude mice is highly reproducible and is ideal for preclinical studies on experimental intravesical therapies.
Li
Chong
C
Department of Immunology, Peking University Health Science Center, Beijing 100083, China.
Yan
Ru-Ping
RP
Yuan
Guo-Hong
GH
Feng
You-Gang
YG
Wang
Jian-Song
JS
Gao
Xiang
X
Huang
Yan-Shan
YS
Zhou
Jin-Bao
JB
Xie
Shu-Sheng
SS
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D002295:Carcinoma, Transitional Cell / Q000276:immunology / Q000473:pathology*; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008279:Magnetic Resonance Imaging; D051379:Mice; D008819:Mice, Nude; D009367:Neoplasm Staging; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000473:pathology; D014183:Transplantation, Heterologous; D001749:Urinary Bladder Neoplasms / Q000276:immunology / Q000473:pathology*
D000911:Antibodies, Monoclonal