A novel gene with antisalt and anticadmium stress activities from a halotolerant marine green alga Chlamydomonas sp. W80.
A novel gene with antistress activities against both salt (NaCl) and cadmium stresses was isolated from the cDNA library of halotolerant green alga Chlamydomonas sp. strain W80 by a functional expression screening with Escherichia coli. The C-terminal region of this protein is responsible for the antistress activity, because N-terminal truncated clone of this gene retains the antistress activity, and the C-terminal truncated clone loses the activity. In the C-terminal region, there is a histidine and aspartic acid-rich domain (HD-rich domain).
Tanaka
Satoshi
S
The Kansai Electric Power Co, Environmental Research Center, Kyoto, Japan.
Suda
Yoshito
Y
Ikeda
Kazunori
K
Ono
Masahiro
M
Miyasaka
Hitoshi
H
Watanabe
Masanori
M
Sasaki
Ken
K
Hirata
Kazumasa
K
D000222:Adaptation, Physiological / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D002104:Cadmium / Q000378:metabolism / Q000494:pharmacology; D002696:Chlamydomonas / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D048028:DNA, Algal / Q000737:chemistry / Q000235:genetics; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D050296:Microbial Viability; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D012965:Sodium Chloride / Q000494:pharmacology; D014871:Water Microbiology
D048028:DNA, Algal; D016054:DNA, Protozoan; D002104:Cadmium; D012965:Sodium Chloride