C57Bl/6 mice are protected from respiratory syncytial virus (RSV) challenge and IL-5 associated pulmonary eosinophilic infiltrates following intranasal immunization with Protollin-eRSV vaccine.
The protective efficacy of an intranasal (IN) Protollin-eRSV vaccine has recently been demonstrated in the RSV-susceptible BALB/c mouse model. Here, we report the safety, immunogenicity and efficacy of Protollin-eRSV vaccine in the relatively resistant C57Bl/6 mouse model. C57Bl/6 mice immunized IN with either two or three doses of Protollin-eRSV produced significant systemic and mucosal RSV-specific antibodies. Mice immunized with the Protollin vaccine displayed polarized Th1 responses with augmented IFNgamma/IL-5 ratios in RSV-restimulated lung and spleen cell preparations compared with animals that received antigen alone. The Protollin-eRSV immunized C57Bl/6 mice were fully protected against challenge without eosinophilic pulmonary pathology observed in the animals immunized with the formalin-inactivated RSV vaccine. This new model will permit us to dissect the respective roles of the TLR2 and TLR4 ligands contained in the vaccine using TLR knock-out animals established on the C57Bl/6 background.
Cyr
Sonya L
SL
McGill Center for Tropical Diseases, Montreal General Hospital, Montreal, Quebec, Canada H3G IA4.
Jones
Taff
T
Stoica-Popescu
Ioana
I
Burt
David
D
Ward
Brian J
BJ
D000281:Administration, Intranasal; D000818:Animals; D004195:Disease Models, Animal; D004804:Eosinophils / Q000502:physiology*; D015848:Interleukin-5 / Q000378:metabolism / Q000502:physiology*; D008168:Lung / Q000276:immunology / Q000382:microbiology / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D022261:Respiratory Syncytial Virus Vaccines / Q000008:administration & dosage* / Q000276:immunology; D018113:Respiratory Syncytial Virus, Human / Q000276:immunology*; D012136:Respiratory Syncytial Viruses
D015848:Interleukin-5; D022261:Respiratory Syncytial Virus Vaccines