title: Analysis of interaction property of bioactive components in Danggui Buxue Decoction with protein by microdialysis coupled with HPLC-DAD-MS.
abstract: Interaction of a commonly used combined prescription of Danggui Buxue Decoction (CPDBD) with protein was studied by microdialysis coupled with HPLC-DAD-MS. Nine compounds in CPDBD were unequivocally identified by comparing with their t(R), MS data and UV spectra with those of reference compounds, and simultaneously quantified. Microdialysis recoveries and binding degrees of 20 compounds in CPDBD with bovine serum albumin (BSA) were determined. Recoveries of microdialysis sampling ranged from 66.9 to 91.5% with RSD below 3.0%, and the binding degrees of those to BSA ranged from 6.3 to 59.8% (0.3mM BSA) and from 6.9 to 86.6% (0.6mM BSA). The results were determined at pH 7.4 and the influence of different pH value was investigated. Furthermore, the binding degrees of eight reference compounds were determined separately under the same conditions, indicating a significant effect of the interaction of compounds with each other on their binding degrees to BSA. By comparing their binding degrees with BSA with those of proven active compounds in CPDBD, i.e. chlorogenic acid (3), ferulic acid (6), ononin (12) and calycosin (16), other five compounds were found to possess potential activities, which were tentatively identified as calycosin-7-O-beta-D-glucoside-6-O-malonate (9), senkyunolide I or H (10), formononetin-7-O-beta-D-glucoside-6-O-malonate (17), and two unknown compounds.
authors: Key Laboratory of Modern Chinese Medicines, Ministry of Education and Department of Pharmacognosy, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing 210009, China.
authors: Xiao-Dong
authors: XD
authors: Wen
authors: Lian-Wen
authors: LW
authors: Qi
authors: Jun
authors: J
authors: Chen
authors: Yue
authors: Y
authors: Song
authors: Ling
authors: L
authors: Yi
authors: Xiao-Wei
authors: XW
authors: Yang
authors: Ping
authors: P
authors: Li
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry / Q000379:methods*; D017551:Microdialysis; D010936:Plant Extracts / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; C477279:danggui buxue decoction