Vaccine efficacy of an attenuated but persistent Mycobacterium tuberculosis cysH mutant.
The emergence of drug-resistant Mycobacterium tuberculosis strains and the widespread occurrence of AIDS demand newer and more efficient control of tuberculosis. The protective efficacy of the current Mycobacterium bovis bacille Calmette-Guérin (BCG) vaccine is highly variable. Therefore, development of an effective new vaccine has gained momentum in recent years. Recently, several M. tuberculosis mutants were tested as potential vaccine candidates in the mouse model of tuberculosis. However, only some of these mutants were able to generate protection equivalent to that of BCG in mice. This study reports the vaccine potential of an attenuated 5'-adenosine phosphosulfate reductase mutant (DeltacysH) of M. tuberculosis. Immunization of mice with either BCG or DeltacysH followed by infection with the virulent M. tuberculosis Erdman strain demonstrated that DeltacysH can generate protection equivalent to that of the BCG vaccine.
Senaratne
Ryan H
RH
School of Public Health, University of California, Berkeley, CA 94720, USA.
Mougous
Joseph D
JD
Department of Chemistry, University of California, Berkeley, CA 94720, USA.
Reader
J Rachel
JR
Comparative Pathology Laboratory, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
Williams
Spencer J
SJ
Department of Chemistry, University of California, Berkeley, CA 94720, USA.
Zhang
Tianjiao
T
School of Public Health, University of California, Berkeley, CA 94720, USA.
Bertozzi
Carolyn R
CR
Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA.|Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.|Department of Chemistry, University of California, Berkeley, CA 94720, USA.
Riley
Lee W
LW
School of Public Health, University of California, Berkeley, CA 94720, USA.
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology; D005260:Female; D005798:Genes, Bacterial / Q000235:genetics; D008168:Lung / Q000382:microbiology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation / Q000235:genetics*; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000276:immunology*; D013154:Spleen / Q000382:microbiology / Q000473:pathology; D032581:Tuberculosis Vaccines / Q000235:genetics / Q000276:immunology*; D014397:Tuberculosis, Pulmonary / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology*
D001426:Bacterial Proteins; D032581:Tuberculosis Vaccines; D014613:Vaccines, Attenuated