Miniaturized sample preparation needle: a versatile design for the rapid analysis of smoking-related compounds in hair and air samples.
Miniaturized needle extraction device has been developed as a versatile sample preparation device designed for the rapid and simple analysis of smoking-related compounds in smokers' hair samples and environmental tobacco smoke. Packed with polymeric particle, the resulting particle-packed needle was employed as a miniaturized sample preparation device for the analysis of typical volatile organic compounds in tobacco smoke. Introducing a bundle of polymer-coated filaments as the extraction medium, the needle was further applied as a novel sample preparation device containing simultaneous derivatization/extraction process of volatile aldehydes. Formaldehyde (FA) and acetaldehyde (AA) in smoker's breath during the smoking were successfully derivatized with two derivatization reagents in the polymer-coated fiber-packed needle device followed by the separation and determination in gas chromatography (GC). Smokers' hair samples were also packed into the needle, allowing the direct extraction of nicotine from the hair sample in a conventional GC injector. Optimizing the main experimental parameters for each technique, successful determination of several smoking-related compounds with these needle extraction methods has been demonstrated.
Saito
Yoshihiro
Y
School of Materials Science, Toyohashi University of Technology, Toyohashi 441-8580, Japan.
Ueta
Ikuo
I
Ogawa
Mitsuhiro
M
Hayashida
Makiko
M
Jinno
Kiyokatsu
K
D000079:Acetaldehyde / Q000032:analysis; D001944:Breath Tests; D002849:Chromatography, Gas; D004781:Environmental Exposure; D004867:Equipment Design; D005557:Formaldehyde / Q000032:analysis; D006197:Hair / Q000737:chemistry*; D006801:Humans; D008904:Miniaturization / Q000295:instrumentation / Q000379:methods; D009339:Needles; D009538:Nicotine / Q000032:analysis; D009930:Organic Chemicals / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012907:Smoking; D014028:Tobacco Smoke Pollution / Q000032:analysis*; D014835:Volatilization
D009930:Organic Chemicals; D014028:Tobacco Smoke Pollution; D005557:Formaldehyde; D009538:Nicotine; D000079:Acetaldehyde