EC-SOD suppresses contact hypersensitivity in mouse skin by impairing Langerhans cell migration.
Extracellular superoxide dismutase (EC-SOD) is primarily a tissue enzyme and has been implicated in the modulation of inflammatory response. The biological role of EC-SOD in skin, however, has rarely been investigated. In this study, we aim to explore the effects of EC-SOD on the inflammatory response in skin by evaluating the contact hypersensitivity response (CHS) in EC-SOD transgenic mice. Transgenic mice with skin-specific expression of EC-SOD were sensitized and challenged with 2,4,6-trinitro-1-chlorobenzene (TNCB), followed by measurement of ear swelling. EC-SOD transgenic mice showed significantly reduced CHS responses compared with wild-type mice. Histological evaluation of the challenged ears of EC-SOD transgenic mice revealed diminished infiltration of inflammatory cells with a failure to induce expression of inflammatory cytokines, such as tumor necrosis factor-alpha and IFN-gamma, on sensitization and challenge with TNCB. Furthermore, Langerhans cell migration to lymph nodes was impaired in EC-SOD transgenic mice. These results indicate that EC-SOD downregulates CHS through inhibition of the inflammatory response, suggesting a possible therapeutic regimen in inflammatory skin diseases.
Na
Kwangmin
K
Laboratory of Dermato-Immunology, College of Medicine, Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul, South Korea.
Kim
Kyoung-Eun
KE
Park
Sang-Tae
ST
Kim
Tae-Yoon
TY
D000818:Animals; D002465:Cell Movement; D003877:Dermatitis, Contact / Q000473:pathology / Q000517:prevention & control*; D007371:Interferon-gamma / Q000096:biosynthesis; D007801:Langerhans Cells / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic; D010853:Picryl Chloride / Q000633:toxicity; D013482:Superoxide Dismutase / Q000502:physiology*; D013601:T-Lymphocytes / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D013482:Superoxide Dismutase; D010853:Picryl Chloride