title: The secreted signaling protein factor C triggers the A-factor response regulon in Streptomyces griseus: overlapping signaling routes.
abstract: Members of the prokaryotic genus Streptomyces produce over 60% of all known antibiotics and a wide range of industrial enzymes. A leading theme in microbiology is which signals are received and transmitted by these organisms to trigger the onset of morphological differentiation and antibiotic production. The small gamma-butyrolactone A-factor is an important autoregulatory signaling molecule in streptomycetes, and A-factor mutants are blocked in development and antibiotic production. In this study we showed that heterologous expression of the 324-amino acid secreted regulatory protein Factor C resulted in restoration of development and enhanced antibiotic production of an A-factor-deficient bald mutant of Streptomyces griseus, although the parental strain lacks an facC gene. Proteome analysis showed that in the facC transformant the production of several secreted proteins that belong to the A-factor regulon was restored. HPLC-MS/MS analysis indicated that this was due to restoration of A-factor production to wild-type levels in the transformant. This indicates a connection between two highly divergent types of signaling molecules and possible interplay between their regulatory networks.
authors: Department of Human Genetics, Faculty of Medicine, Medical and Health Science Center, University of Debrecen, Nagyerdei körút 98, H-4032 Debrecen, Hungary.
authors: Zsuzsanna
authors: Z
authors: Birkó
authors: Sylwia
authors: S
authors: Bialek
authors: Krisztina
authors: K
authors: Buzás
authors: Emília
authors: E
authors: Szájli
authors: Bjørn A
authors: BA
authors: Traag
authors: Katalin F
authors: KF
authors: Medzihradszky
authors: Sebastien
authors: S
authors: Rigali
authors: Erik
authors: E
authors: Vijgenboom
authors: András
authors: A
authors: Penyige
authors: Zoltán
authors: Z
authors: Kele
authors: Gilles P
authors: GP
authors: van Wezel
authors: Sándor
authors: S
authors: Biró
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000235:genetics / Q000502:physiology; D001426:Bacterial Proteins / Q000502:physiology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D020543:Proteome / Q000032:analysis; D018085:Regulon; D015398:Signal Transduction; D013305:Streptomyces griseus / Q000235:genetics / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; C402566:factor C, Streptomyces; C025035:A-factor (Streptomyces); D015107:4-Butyrolactone