title: Study on the in vitro effect of zearalenone and alpha-zearalenol on boar sperm-zona pellucida interaction by hemizona assay application.
abstract: The mycotoxin zearalenone (zen) impairs fertility in farm animals. The aim of the present study was to investigate the effect of zearalenone and its major metabolite (alpha-zearalenol) on boar semen binding capacity, under in vitro conditions. Extended boar semen was exposed to three different concentrations of zen and alpha-zen (40, 60 and 80 microg ml(-1) of semen) for 1 h. Afterwards, the semen was washed and incubated with homologous oocyte hemizona for 4 h. A significant decrease (P < 0.001) in the number of tightly attached spermatozoa on the hemizona was obtained at concentrations of 60 microg ml(-1) and 80 microg ml(-1) of zen and alpha-zen. In conclusion, zen and alpha-zen affected the sperm-zona interaction by reducing the ability of boar spermatozoa to bind to the zona pellucida.
authors: NAGREF, Veterinary Research Institute, Ionia, 57008 Thessaloniki, Greece.
authors: I A
authors: IA
authors: Tsakmakidis
authors: A G
authors: AG
authors: Lymberopoulos
authors: E
authors: E
authors: Vainas
authors: C M
authors: CM
authors: Boscos
authors: S C
authors: SC
authors: Kyriakis
authors: C
authors: C
authors: Alexopoulos
mesh_terms: D000818:Animals; D005260:Female; D008297:Male; D013081:Sperm Motility / Q000187:drug effects; D013084:Sperm-Ovum Interactions / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology; D013552:Swine; D015025:Zearalenone / Q000633:toxicity*; D015029:Zeranol / Q000031:analogs & derivatives* / Q000633:toxicity; D015044:Zona Pellucida / Q000187:drug effects* / Q000502:physiology
chemical_list: C029659:zearalenol; D015025:Zearalenone; D015029:Zeranol