A human homolog of mouse Lbh gene, hLBH, expresses in heart and activates SRE and AP-1 mediated MAPK signaling pathway.
It has been reported that mouse Lbh (limb-bud and heart) can regulate cardiac gene expression by modulating the combinatorial activities of key cardiac transcription factors, as well as their individual functions in cardiogenesis. Here we report the cloning and characterization of the human homolog of mouse Lbh gene, hLBH, from a human embryonic heart cDNA library. The cDNA of hLBH is 2927 bp long, encoding a protein product of 105 amino acids. The protein is highly conserved in evolution across different species from zebra fish, to mouse, to human. Northern blot analysis indicates that a 2.9 kb transcript specific for hLBH is most abundantly expressed in both embryonic and adult heart tissue. In COS-7 cells, hLBH proteins are localized to both the nucleus and the cytoplasm. hLBH is a transcription activator when fused to Gal-4 DNA-binding domain. Deletion analysis indicates that both the N-terminal containing proline-dependent serine/threonine kinase group and the C-terminal containing ERK D-domain motif are required for transcriptional activation. Overexpression of hLBH in COS-7 cells activates the transcriptional activities of activator protein-1 (AP-1) and serum response element (SRE). These results suggest that hLBH proteins may act as a transcriptional activator in mitogen-activated protein kinase signaling pathway to mediate cellular functions.
Ai
Jianping
J
The Center For Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University, Changsha, Hunan, 410081, Peoples' Republic of China.
Wang
Yuequn
Y
Tan
Kunrong
K
Deng
Yun
Y
Luo
Na
N
Yuan
Wuzhou
W
Wang
Zequn
Z
Li
Yongqing
Y
Wang
Ying
Y
Mo
Xiaoyang
X
Zhu
Chuanbing
C
Yin
Zhaochu
Z
Liu
Mingyao
M
Wu
Xiushan
X
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D018797:Cell Cycle Proteins; D002522:Chlorocebus aethiops; D017124:Conserved Sequence; D019143:Evolution, Molecular; D018507:Gene Expression Regulation, Developmental; D015894:Genome, Human / Q000235:genetics; D006801:Humans; D020935:MAP Kinase Signaling System; D051379:Mice; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics*; D010802:Phylogeny; D021381:Protein Transport; D017386:Sequence Homology, Amino Acid; D027602:Serum Response Element / Q000235:genetics*; D013347:Subcellular Fractions / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D018808:Transcription Factor AP-1 / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation / Q000235:genetics
D018797:Cell Cycle Proteins; C531176:LBH protein, human; C428223:Lbh protein, mouse; D009687:Nuclear Proteins; D015534:Trans-Activators; D018808:Transcription Factor AP-1; D014157:Transcription Factors