title: Synthesis of Zn(II)-cloxacillin sodium complex and study of its interaction with calf thymus DNA.
abstract: In this paper, a solid complex of cloxacillin sodium (CS) with Zn(II) was prepared by coprecipitation and characterized by UV, fluorescence, IR, and thermal spectra. Furthermore, the nature of the complex has been studied by 1H-NMR and 13C-NMR spectroscopy. The influence of Zn(II) on the combination of CS and calf thymus DNA (CT DNA) was studied using fluorescence spectrophotometry, and the formation of binary CS-Zn(II) and CS-CT DNA complexes and ternary CS-Zn(II)-CT DNA complex was studied. The results show that the fluorescence intensity of CS can be quenched in the presence of Zn(II) or DNA. In the presence of Zn(II), the fluorescence quenching action of DNA on CS was strongly enhanced. Based on the fluorescence intensity, the formation constants of CS-Zn(II) and CS-CT DNA complexes were calculated, and the mechanism of interaction between CS, Zn(II), and DNA is discussed.
authors: The Institute of Modern Chemistry, Shanxi University, Shanxi, PR China. chao@sxu.edu.cn
authors: Jian-Bin
authors: JB
authors: Chao
authors: Meng-Dan
authors: MD
authors: Xu
authors: Cai-Xia
authors: CX
authors: Yin
authors: Shuping
authors: S
authors: Huang
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism; D003023:Cloxacillin / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D005453:Fluorescence; D008860:Microscopy, Ultraviolet; D015394:Molecular Structure; D013055:Spectrophotometry, Infrared; D015032:Zinc / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D004247:DNA; C427203:calf thymus DNA; D015032:Zinc; D003023:Cloxacillin