title: Molecular characterization of CEVd strains that induce different phenotypes in Gynura aurantiaca: structure-pathogenicity relationships.
abstract: Two Citrus exocortis viroid isolates (CEVd-s and CEVd-129) that induce severe and mild symptoms in Gynura aurantiaca, respectively, have been characterized. They present nucleotide sequences in the pathogenicity motifs P(L), C and P(R) similar to those of "Class A" and "Class B". Infectivity and symptom expression in G. aurantica and tomato were evaluated with a selection of sequence variants recovered from both isolates. As expected, the two variants selected from CEVd-s induced severe symptoms. The variants selected from CEVd-129 induced mild symptoms, except one of them, named MJ, that presented an unusual genotype and induced severe symptoms in G. aurantiaca. The biological properties of MJ show that the two nucleotide changes of the C domain normally associated with the P(L) and P(R) motifs of "Class B" strains are not implicated in symptom expression. The relationship between "Class A" and "Class B" strains with the symptoms induced in clementine trees grafted on trifoliate orange is discussed.
authors: Departamento de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Moncada, Spain.
authors: M
authors: M
authors: Chaffai
authors: P
authors: P
authors: Serra
authors: M
authors: M
authors: Gandía
authors: C
authors: C
authors: Hernández
authors: N
authors: N
authors: Duran-Vila
mesh_terms: D019659:Asteraceae / Q000821:virology*; D001483:Base Sequence; D002957:Citrus / Q000821:virology; D004279:DNA, Viral / Q000235:genetics; D014644:Genetic Variation; D018551:Solanum lycopersicum / Q000821:virology; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010641:Phenotype; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D018807:Polymorphism, Single-Stranded Conformational; D032104:Poncirus / Q000821:virology; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D014772:Viroids / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity*; D014774:Virulence / Q000235:genetics
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral