Hydroxypropyl-beta-cyclodextrin enhanced determination for the Vitamin B12 by fluorescence quenching method.
A novel fluorescence quenching method for the determination of Vitamin B12(VB12) had been developed. It was based on that the fluorescence intensity of erythrosine sodium(ES) could be enhanced by Hydroxypropyl-beta-cyclodextrin(HP-beta-CD) due to the formation of inclusion complex (HP-beta-CD-ES), while the fluorescence intensity of HP-beta-CD-ES was diminished after adding VB12 into the system, and there was a linear relationship between the fluorescence quenching value of the system (DeltaF) and the concentration of VB12 (c). The mechanism of the determination of VB12 was discussed. The results showed that under the optimal conditions, the linear range of calibration curve for the determination of VB12 was 0.0 approximately 2.1 x 10(-5) mol/L, and the detection limit was 1.8 x 10(-7) mol/ L. It could be satisfactorily applied to the determination of VB12 in injections.
Sun
Jing
J
College of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou 225009, China.
Zhu
Xiashi
X
Wu
Ming
M
D000073738:2-Hydroxypropyl-beta-cyclodextrin; D000596:Amino Acids / Q000737:chemistry; D002138:Calibration; D002412:Cations / Q000737:chemistry; D004923:Erythrosine / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014805:Vitamin B 12 / Q000032:analysis* / Q000737:chemistry; D014815:Vitamins / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry*
D000596:Amino Acids; D002412:Cations; D014815:Vitamins; D047392:beta-Cyclodextrins; D000073738:2-Hydroxypropyl-beta-cyclodextrin; D014805:Vitamin B 12; D004923:Erythrosine