title: Automated ion channel screening: patch clamping made easy.
abstract: Efficient high resolution techniques are required for screening efforts and research targeting ion channels. The conventional patch clamp technique, a high resolution but low efficiency technique, has been established for 25 years. Recent advances have opened up new possibilities for automated patch clamping. This new technology meets the need of drug developers for higher throughput and facilitates new experimental approaches in ion channel research. Specifically, Nanion's electrophysiology workstations, the Port-a-Patch and the Patchliner, have been successfully introduced as high-quality automated patch clamp platforms for industry as well as academic users. Both platforms give high quality patch clamp recordings, capable of true giga-seals and stable recordings, accessible to the user without the need for years of practical training. They also offer sophisticated experimental possibilities, such as accurate and fast ligand application, temperature control and internal solution exchange. This article describes the chip-based patch clamp technology and its usefulness in ion channel drug screening and academic research.
authors: Nanion Technologies GmbH, Erzgiessereistr. 4, 80335 Munich, Germany.
authors: Cecilia
authors: C
authors: Farre
authors: Sonja
authors: S
authors: Stoelzle
authors: Claudia
authors: C
authors: Haarmann
authors: Michael
authors: M
authors: George
authors: Andrea
authors: A
authors: Brüggemann
authors: Niels
authors: N
authors: Fertig
mesh_terms: D001331:Automation / Q000295:instrumentation / Q000379:methods; D002460:Cell Line; D004594:Electrophysiology / Q000295:instrumentation / Q000379:methods; D006801:Humans; D007473:Ion Channels / Q000378:metabolism*; D018408:Patch-Clamp Techniques / Q000295:instrumentation* / Q000379:methods*
chemical_list: D007473:Ion Channels