Electrochemical detection of phenolic compounds using cylindrical carbon-ink electrodes and microchip capillary electrophoresis.
A simple method to fabricate cylindrical carbon electrodes for use in capillary electrophoresis (CE) microchips is described. The electrodes were fabricated using a metallic wire coated with carbon ink. Several experimental variables were studied in order to establish the best conditions to fabricate the electrode. Finally, the electrodes were integrated in a poly(dimethylsiloxane) microchip and used for the analysis of phenolic compounds. Using the optimum conditions, the analysis of a mixture of dopamine, epinephrine, catechol, and 4-aminophenol was achieved in less than 240 s, showing good linear responses (R(2)=0.999) in the 0.1-190 microM range, and limits of detection (without the use of stacking or a decoupler) of 140 and 105 nM for dopamine and epinephrine, respectively.
Ding
Yongsheng
Y
Department of Chemistry, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, United States.
Ayon
Arturo
A
García
Carlos D
CD
D002244:Carbon / Q000737:chemistry*; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D046209:Electrophoresis, Microchip / Q000295:instrumentation*; D004867:Equipment Design; D006046:Gold / Q000737:chemistry; D007281:Ink; D010636:Phenols / Q000032:analysis*; D010984:Platinum / Q000737:chemistry; D012828:Silicones / Q000737:chemistry
D004129:Dimethylpolysiloxanes; D010636:Phenols; D012828:Silicones; D010984:Platinum; C013830:baysilon; D002244:Carbon; D006046:Gold