Gradients of antigen expression and developmental potential in hematopoiesis.
Prospective isolation of hematopoietic stem and progenitor cell subsets depends upon the premise that expression of combinations of surface antigens reflects developmental potential. During the process of differentiation, however, the loss of antigens associated with stem cells and the concomitant gain of those associated with progenitor cells often occurs as a continuum rather than by discrete binary steps. Coupled with the fact that assay conditions can profoundly influence the developmental fates of prospectively isolated cells, gradients of antigen expression during differentiation have led to a variety of interpretations of lineage commitment in hematopoiesis.
Kwak
Jae-Yong
JY
Division of Hematology/Oncology, Department of Internal Medicine, Chonbuk National University Medical School, Jeonju 561-712, Korea.
Cho
Scott
S
Spangrude
Gerald J
GJ
D000818:Animals; D000941:Antigens / Q000096:biosynthesis* / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology; D002469:Cell Separation; D004920:Erythropoiesis; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D018507:Gene Expression Regulation, Developmental; D006410:Hematopoiesis; D008214:Lymphocytes / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D013234:Stem Cells / Q000166:cytology; D013997:Time Factors
D000941:Antigens