title: Bacillus subtilis strain deficient for the protein-tyrosine kinase PtkA exhibits impaired DNA replication.
abstract: Bacillus subtilis has recently come into the focus of research on bacterial protein-tyrosine phosphorylation, with several proteins kinases, phosphatases and their substrates identified in this Gram-positive model organism. B. subtilis protein-tyrosine phosphorylation system PtkA/PtpZ was previously shown to regulate the phosphorylation state of UDP-glucose dehydrogenases and single-stranded DNA-binding proteins. This promiscuity towards substrates is reminiscent of eukaryal kinases and has prompted us to investigate possible physiological effects of ptkA and ptpZ gene inactivations in this study. We were unable to identify any striking phenotypes related to control of UDP-glucose dehydrogenases, natural competence and DNA lesion repair; however, a very strong phenotype of DeltaptkA emerged with respect to DNA replication and cell cycle control, as revealed by flow cytometry and fluorescent microscopy. B. subtilis cells lacking the kinase PtkA accumulated extra chromosome equivalents, exhibited aberrant initiation mass for DNA replication and an unusually long D period.
authors: Center for Microbial Biotechnology, BioCentrum, Technical University of Denmark, DK-2800 Lyngby, Denmark.
authors: Dina
authors: D
authors: Petranovic
authors: Ole
authors: O
authors: Michelsen
authors: Ksenija
authors: K
authors: Zahradka
authors: Catarina
authors: C
authors: Silva
authors: Mirjana
authors: M
authors: Petranovic
authors: Peter Ruhdal
authors: PR
authors: Jensen
authors: Ivan
authors: I
authors: Mijakovic
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics* / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000235:genetics; D005434:Flow Cytometry; D015964:Gene Expression Regulation, Bacterial; D011505:Protein-Tyrosine Kinases / Q000172:deficiency* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D011505:Protein-Tyrosine Kinases