[Study of loss of heterozygosity in oligodendroglial tumors by real-time quantitative polymerase chain reaction-based microsatellite analysis].
OBJECTIVE
To study the loss of heterozygosity (LOH) at chromosomes 1p or 19q in oligodendroglial tumors.


METHODS
Twenty-eight cases of oligodendroglial tumors were enrolled into the study. Real-time quantitative polymerase chain reaction-based microsatellite analysis was performed on paraffin-embedded tumor tissues in order to study the status of chromosomes 1p and 19q.


RESULTS
Among the 28 cases of oligodendroglial tumors, 24 cases (85.7%) showed 1p LOH, while 18 cases (64.3%) showed 19q LOH and 17 cases (60.7%) showed LOH of both 1p and 19q. LOH at 1p or 19q was present in 25 (89.3%) of the 28 cases.


CONCLUSIONS
Real-time quantitative polymerase chain reaction-based microsatellite analysis is a rapid and specific way in detecting LOH in paraffin-embedded tumor tissues. LOH at 1p or 19q is present in majority of the oligodendroglial tumors studied.
Zhang
Shu-kun
SK
Department of Pathology, Xuanwu Hospital, Capital University of Medical Sciences, Beijing 100053, China.
Lu
De-hong
DH
Piao
Yue-shan
YS
Cai
Yan-ning
YN
Xu
Qing-zhong
QZ
D000328:Adult; D001932:Brain Neoplasms / Q000235:genetics*; D002878:Chromosomes, Human, Pair 1; D002888:Chromosomes, Human, Pair 19; D005260:Female; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D009837:Oligodendroglioma / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*