Cytotoxic withanolides from Physalis angulata L.
Four new withanolides, physagulins L-O (1-4), were isolated from the MeOH extract of the aerial parts of Physalis angulata L. (Solanaceae), together with seven known withanolides, compounds 5-11. Their structures were determined by spectroscopic techniques, including 1H-, 13C-NMR (DEPT), and 2D-NMR (HMBC, HMQC, 1H,1H-COSY, NOESY) experiments, as well as by HR-MS. All eleven compounds were tested for their antiproliferative activities towards human colorectal-carcinoma (HCT-116) and human non-small-cell lung-cancer (NCI-H460) cells. Compound 5 exhibited the highest anticancer activity against the HCT-116 cell line, with an IC50 value of 1.64+/-0.06 microM. Compound 9 exhibited the highest cytotoxicity towards the NCI-H460 cell line, with an IC50 value of 0.43+/-0.02 microM.
He
Qing-Ping
QP
Shanghai Institute of Materia Medica, Shanghai 201203, PR. China.
Ma
Lei
L
Luo
Jie-Ying
JY
He
Fu-Yuan
FY
Lou
Li-Guang
LG
Hu
Li-Hong
LH
D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification; D045744:Cell Line, Tumor; D003603:Cytotoxins / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D032321:Physalis; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D032341:Withania
D000970:Antineoplastic Agents; D003603:Cytotoxins; D010936:Plant Extracts