Impact of 3,4-dideoxyglucosone-3-ene (3,4-DGE) on cytotoxicity of acidic heat-sterilized peritoneal dialysis fluid.
Of the glucose degradation products (GDPs) in glucose-rich peritoneal dialysate, we investigated the influence of 3,4-dideoxyglucosone-3-ene (3,4-DGE) on the cytotoxicity of acidic heat-sterilized peritoneal dialysis fluid (L-H PDF) using human peritoneal mesothelial cells (HPMC). We prepared acidified filtration-sterilized PDF (glucose concentration 3.86%) containing eight types of added GDP [3,4-DGE, glyoxal (GO), methylglyoxal (MGO), 3-deoxyglucosone (3-DG), formaldehyde (FA), acetaldehyde (AA), 5-hydroxymethyl-2-furaldehyde (5-HMF), and furfural (FF)] or seven types of GDP (GO, MGO, 3-DG, FA, AA, 5-HMF, and FF). HPMC were exposed to these two types of solution and acidic heat-sterilized PDF (glucose concentration 3.86%, L-H 3.86) for 4 h. Cell viability was determined by 3,(4,5-dimethythiazol-2-yl)2,5-diphenyl-terazolium bromide (MTT) assay. MTT viability was decreased significantly compared with the control when treated with L-H 3.86 or acidified neutral filtration-sterilized PDF (glucose concentration 3.86%) containing eight GDPs. However, no significant decrease in MTT viability was observed when HPMC were treated with acidified neutral filtration-sterilized PDF (glucose concentration 3.86%) containing seven GDPs. Thus, 3,4-DGE strongly affects the cytotoxicity of L-H PDF. It is suggested that the cytotoxicity of L-H PDF is based on the presence of 3,4-DGE.
Tomo
Tadashi
T
Second Department of Internal Medicine, Faculty of Medicine, Oita University, Yufu, Oita, Japan. tomo@med.oita-u.ac.jp
Okabe
Eiji
E
Yamamoto
Takashi
T
Namoto
Shinji
S
Iwashita
Tomohiko
T
Matsuyama
Kazuhiro
K
Kadota
Jun-ichi
J
D015314:Dialysis Solutions; D015312:Hemodialysis Solutions / Q000633:toxicity*; D006801:Humans; D010530:Peritoneal Dialysis; D010537:Peritoneum / Q000166:cytology; D011753:Pyrones / Q000633:toxicity*; D013242:Sterilization; D013778:Tetrazolium Salts; D013844:Thiazoles; D018675:Toxicity Tests
C504711:3,4-dideoxyglucosone-3-ene; D015314:Dialysis Solutions; D015312:Hemodialysis Solutions; D011753:Pyrones; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue