Release of thioredoxin from Saccharomyces cerevisiae with environmental stimuli: solubilization of thioredoxin with ethanol.
Thioredoxin is crucial for the maintenance of the redox status of cells of all types. Mammalian thioredoxin is secreted from various types of cells, although the mechanism underlying has not yet been clarified. Previously, we demonstrated that thioredoxin was released from Saccharomyces cerevisiae after treatment with ethanol. In this paper, we show that as well as ethanol, low-pH shock and hypoosmotic shock release thioredoxin. Low-molecular-weight proteins in yeast cells were preferentially released by treatment with ethanol and low-pH shock. A cell wall integrity pathway seems partially involved in the hypoosmotic shock-induced release of thioredoxin. Considerable amounts of thioredoxin were present in the insoluble fractions of the cells, a portion of which was associated with lipid microdomains that are resistant to nonionic detergent at 4 degrees C. The intracellular localization of thioredoxin may influence the efficiency of its release from yeast cells with ethanol.
Takeuchi
Yoko
Y
Laboratory of Molecular Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan.
Nomura
Wataru
W
Ohdate
Takumi
T
Tamasu
Shogo
S
Masutani
Hiroshi
H
Murata
Kazuo
K
Izawa
Shingo
S
Yodoi
Junji
J
Inoue
Yoshiharu
Y
D000431:Ethanol / Q000378:metabolism*; D009997:Osmotic Pressure; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D012995:Solubility; D013879:Thioredoxins / Q000378:metabolism*
D000431:Ethanol; D013879:Thioredoxins