Covalent bonding of vancomycin to Ti6Al4V alloy pins provides long-term inhibition of Staphylococcus aureus colonization.
Self-protecting Ti6Al4V alloy pins were prepared by covalent bonding of bis(ethylene glycol) linkers, then vancomycin to the oxidized, aminopropylated Ti6Al4V alloy surface. Fluorescence modification-enabled estimation of yields of free amines on the metallic surface monolayer at each reaction step. The vancomycin-protected Ti6Al4V pins were not colonized by Staphylococcus aureus, even after 44days storage in physiological buffer. These results provide a basis for testing self-protection against S. aureus colonization in animal models.
Edupuganti
Om P
OP
Department of Biochemistry & Molecular Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA.
Antoci
Valentin
V
King
Samuel B
SB
Jose
Binoy
B
Adams
Christopher S
CS
Parvizi
Javad
J
Shapiro
Irving M
IM
Zeiger
Allen R
AR
Hickok
Noreen J
NJ
Wickstrom
Eric
E
D000497:Alloys; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development; D013995:Time; D014025:Titanium / Q000737:chemistry*; D014640:Vancomycin / Q000737:chemistry / Q000494:pharmacology*
D000497:Alloys; C031462:titanium alloy (TiAl6V4); D014640:Vancomycin; D014025:Titanium