Lack of preventive effects of dietary fibers or chlorophyllin against acrylamide toxicity in rats.
Dietary fibers and chlorophyllin have shown to exert anti-carcinogenic effects against co-administered carcinogens. To test the possibility of chemoprevention by such dietary supplements on subacutely induced acrylamide (ACR) toxicity, Sprague-Dawley male rats were administered 2.5% sodium alginate, 5% glucomannan, 5% digestion resistant maltodextrin, 2.5% chitin or 1% chlorophyllin in the diet, and starting one week later, co-administered 0.02% ACR in the drinking water for 4 weeks. For comparison, untreated control animals given basal diet and tap water were also included. Neurotoxicity was examined with reference to gait abnormalities and by quantitative assessment of histopathological changes in the sciatic and trigeminal nerves, as well as aberrant dot-like immunoreactivity for synaptophysin in the cerebellar molecular layer. Testicular toxicity was assessed by quantitation of seminiferous tubules with exfoliation of germ cells into the lumen and cell debris in the ducts of the epididymides. Development of testicular toxicity as well as neurotoxicity was evident with ACR-treatment, but was not suppressed by dietary addition of fibers or chlorophyllin, suggesting no apparent beneficial influence of these dietary supplements on experimentally induced subacute ACR toxicity.
Woo
Gye-Hyeong
GH
Division of Pathology, National Institute of Health Sciences, 1-18-1 Kamiyoga, Tokyo 158-8501, Japan.
Shibutani
Makoto
M
Kuroiwa
Keiko
K
Lee
Kyoung-Youl
KY
Takahashi
Miwa
M
Inoue
Kaoru
K
Fujimoto
Hitoshi
H
Hirose
Masao
M
D020106:Acrylamide / Q000633:toxicity*; D000464:Alginates / Q000494:pharmacology; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002686:Chitin / Q000494:pharmacology; D002735:Chlorophyllides / Q000494:pharmacology*; D004043:Dietary Fiber / Q000494:pharmacology*; D004326:Drinking / Q000187:drug effects; D005684:Gait / Q000187:drug effects; D020723:Glucuronic Acid / Q000494:pharmacology; D006603:Hexuronic Acids / Q000494:pharmacology; D006651:Histocytochemistry; D008297:Male; D008351:Mannans / Q000494:pharmacology; D009422:Nervous System Diseases / Q000139:chemically induced* / Q000473:pathology / Q000517:prevention & control*; D009929:Organ Size / Q000187:drug effects; D011134:Polysaccharides / Q000494:pharmacology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013737:Testis / Q000473:pathology
D000464:Alginates; D002735:Chlorophyllides; D004043:Dietary Fiber; D006603:Hexuronic Acids; D008351:Mannans; D011134:Polysaccharides; D002686:Chitin; D020106:Acrylamide; C022901:(1-6)-alpha-glucomannan; C008315:maltodextrin; D020723:Glucuronic Acid; C007020:chlorophyllin