Confirmation of four nitroimidazoles in porcine liver by liquid chromatography-tandem mass spectrometry.
A sensitive and reliable multiresidue method is described for analysis of ronidazole, metronidazole, dimetridazole and the common metabolite of ronidazole and dimetridazole, 2-hydroxymethyl-1-methyl-5-nitroimidazole in swine liver. The sample preparation procedure was based on liquid-liquid extraction and mixed mode cation exchange/reverse phase solid-phase extraction. The compounds of interest were determined by reverse phase gradient liquid chromatography separation and tandem mass spectrometry (MS/MS) in the multiple reaction monitoring (MRM) mode. The limits of confirmation were 0.1-0.5 microg kg(-1) for the analytes.
Xia
X
X
China Agricultural University, College of Veterinary Medicine, Department of Pharmacology and Toxicology, Beijing 100094, China.
Li
X
X
Zhang
S
S
Ding
S
S
Jiang
H
H
Shen
J
J
D000818:Animals; D002138:Calibration; D002412:Cations; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D004131:Dimetridazole / Q000032:analysis; D004350:Drug Residues / Q000032:analysis; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods; D008795:Metronidazole / Q000031:analogs & derivatives / Q000032:analysis; D009593:Nitroimidazoles / Q000032:analysis*; D015203:Reproducibility of Results; D012385:Ronidazole / Q000032:analysis; D013552:Swine
D002412:Cations; D009593:Nitroimidazoles; D008795:Metronidazole; C059098:2-hydroxymethyl-1-methyl-5-nitroimidazole; D012385:Ronidazole; D004131:Dimetridazole