Casiopeina III-ia induces apoptosis in HCT-15 cells in vitro through caspase-dependent mechanisms and has antitumor effect in vivo.
The aim of this study was to evaluate the in vitro and in vivo effects of the new chemotherapy agent Casiopeina III-ia [(4,4'-dimethyl-2,2'-bipiridine)(acetylacetonate) Copper (II) nitrate] on HCT-15 (p53-/-) colon cellular line. In vitro, the drug reduced the viability and induced necrosis and apoptosis in a dose dependent manner, without affecting cell cycle phases. Apoptosis was related to Bax increasing levels, suggesting a caspase-dependent mechanism of death, as verified by nucleosomal fragmentation of DNA. In vivo, the antitumor activity of Casiopeina III-ia was tested in HCT-15 cells transplanted to nude mice. In this study we will show that the novel antineoplastic agent Casiopeina III-ia is active on this colon tumor line, setting out as a good candidate for the treatment of colon tumors refractory to chemotherapy.
Carvallo-Chaigneau
Francisco
F
Department of Pathology, Faculty of Veterinary Medicine and Husbandry, Universidad Nacional Autónoma de México, Circuito interior 3000, Delegacion Coyoacan, Mexico, DF, 04510 Mexico. francisco.carvallo@gmail.com
Trejo-Solís
Cristina
C
Gómez-Ruiz
Celedonio
C
Rodríguez-Aguilera
Ernesto
E
Macías-Rosales
Lucía
L
Cortés-Barberena
Edith
E
Cedillo-Peláez
Carlos
C
Gracia-Mora
Isabel
I
Ruiz-Azuara
Lena
L
Madrid-Marina
Vicente
V
Constantino-Casas
Fernando
F
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D001835:Body Weight / Q000187:drug effects; D020169:Caspases / Q000378:metabolism*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009942:Organometallic Compounds / Q000737:chemistry / Q000494:pharmacology*; D023041:Xenograft Model Antitumor Assays; D051028:bcl-2-Associated X Protein / Q000378:metabolism
C525246:(4,4-dimethyl-2,2-bipyridine)(acetylacetonate)copper(II); D009942:Organometallic Compounds; D051028:bcl-2-Associated X Protein; D020169:Caspases