title: HIV patients developing primary CNS lymphoma lack EBV-specific CD4+ T cell function irrespective of absolute CD4+ T cell counts.
abstract: BACKGROUND
  In chronic HIV infection, antiretroviral therapy-induced normalization of CD4(+) T cell counts (immune reconstitution [IR]) is associated with a decreased incidence of opportunistic diseases. However, some individuals remain at risk for opportunistic diseases despite prolonged normalization of CD4(+) T cell counts. Deficient Epstein-Barr virus (EBV)-specific CD4(+) T cell function may explain the occurrence of EBV-associated opportunistic malignancy-such as primary central nervous system (PCNS) lymphoma-despite recovery of absolute CD4(+) T cell counts.
  
  
  RESULTS
  Absolute CD4(+) T cell counts and EBV-specific CD4(+) T cell-dependent interferon-gamma production were assessed in six HIV-positive individuals prior to development of PCNS lymphoma ("cases"), and these values were compared with those in 16 HIV-infected matched participants with no sign of EBV-associated pathology ("matched controls") and 11 nonmatched HIV-negative blood donors. Half of the PCNS lymphoma patients fulfilled IR criteria (defined here as CD4(+) T cell counts >or=500/microl blood). EBV-specific CD4(+) T cells were assessed 0.5-4.7 y prior to diagnosis of lymphoma. In 0/6 cases versus 13/16 matched controls an EBV-specific CD4(+) T cell response was detected (p = 0.007; confidence interval for odds ratio [0-0.40]). PCNS lymphoma patients also differed with regards to this response significantly from HIV-negative blood donors (p < 0.001, confidence interval for odds ratio [0-0.14]), but there was no evidence for a difference between HIV-negative participants and the HIV-positive matched controls (p = 0.47).
  
  
  CONCLUSIONS
  Irrespective of absolute CD4(+) T cell counts, HIV-positive patients who subsequently developed PCNS lymphoma lacked EBV-specific CD4(+) T cell function. Larger, ideally prospective studies are needed to confirm these preliminary data, and clarify the impact of pathogen-specific versus surrogate marker-based assessment of IR on clinical outcome.
authors: Immunobiology Laboratory, Department of Research, University Hospital Basel, Basel, Switzerland.
authors: Olivier
authors: O
authors: Gasser
authors: Florian K
authors: FK
authors: Bihl
authors: Marcel
authors: M
authors: Wolbers
authors: Elisabetta
authors: E
authors: Loggi
authors: Ingrid
authors: I
authors: Steffen
authors: Hans H
authors: HH
authors: Hirsch
authors: Huldrych F
authors: HF
authors: Günthard
authors: Bruce D
authors: BD
authors: Walker
authors: Christian
authors: C
authors: Brander
authors: Manuel
authors: M
authors: Battegay
authors: Christoph
authors: C
authors: Hess
mesh_terms: D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology* / Q000821:virology*; D016022:Case-Control Studies; D016543:Central Nervous System Neoplasms / Q000150:complications* / Q000821:virology*; D002908:Chronic Disease; D003587:Cytomegalovirus / Q000378:metabolism; D005260:Female; D015658:HIV Infections / Q000150:complications*; D004854:Herpesvirus 4, Human / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D008223:Lymphoma / Q000150:complications* / Q000821:virology*; D008297:Male; D008875:Middle Aged; D019562:Viral Load
chemical_list: D007371:Interferon-gamma