Panmicrobial oligonucleotide array for diagnosis of infectious diseases.
To facilitate rapid, unbiased, differential diagnosis of infectious diseases, we designed GreeneChipPm, a panmicrobial microarray comprising 29,455 sixty-mer oligonucleotide probes for vertebrate viruses, bacteria, fungi, and parasites. Methods for nucleic acid preparation, random primed PCR amplification, and labeling were optimized to allow the sensitivity required for application with nucleic acid extracted from clinical materials and cultured isolates. Analysis of nasopharyngeal aspirates, blood, urine, and tissue from persons with various infectious diseases confirmed the presence of viruses and bacteria identified by other methods, and implicated Plasmodium falciparum in an unexplained fatal case of hemorrhagic feverlike disease during the Marburg hemorrhagic fever outbreak in Angola in 2004-2005.
Palacios
Gustavo
G
Columbia University, New York, New York 10032, USA.
Quan
Phenix-lan
PL
Jabado
Omar J
OJ
Conlan
Sean
S
Hirschberg
David L
DL
Liu
Yang
Y
Zhai
Junhui
J
Renwick
Neil
N
Hui
Jeffrey
J
Hegyi
Hedi
H
Grolla
Allen
A
Strong
James E
JE
Towner
Jonathan S
JS
Geisbert
Thomas W
TW
Jahrling
Peter B
PB
Büchen-Osmond
Cornelia
C
Ellerbrok
Heinz
H
Sanchez-Seco
Maria Paz
MP
Lussier
Yves
Y
Formenty
Pierre
P
Nichol
M Stuart T
MS
Feldmann
Heinz
H
Briese
Thomas
T
Lipkin
W Ian
WI
D003141:Communicable Diseases / Q000175:diagnosis* / Q000821:virology; D004196:Disease Outbreaks; D017809:Fatal Outcome; D006801:Humans; D016778:Malaria, Falciparum / Q000175:diagnosis; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods*; D010802:Phylogeny; D012680:Sensitivity and Specificity; D014777:Virus Diseases / Q000175:diagnosis* / Q000821:virology