title: Hemodialysis vintage, black ethnicity, and pretransplantation antidonor cellular immunity in kidney transplant recipients.
abstract: Prolonged exposure to dialysis before transplantation and black ethnicity are known risk factors for acute rejection and graft loss in kidney transplant recipients. Because the strength of the primed antidonor T cell repertoire before transplantation also is associated with rejection and graft dysfunction, this study sought to determine whether hemodialysis (HD) vintage and/or black ethnicity affected donor-directed T cell immunity. An enzyme-linked immunosorbent spot (ELISPOT) assay was used to measure the frequency of peripheral T cells that expressed IFN-gamma in response to donor stimulator cells before transplantation in 100 kidney recipients. Acute rejection occurred in 38% of ELISPOT (+) patients versus 14% of ELISPOT (-) patients (P = 0.008). The median (HD) vintage was 46 mo (0 to 125 mo) in ELISPOT (+) patients versus 24 mo (0 to 276 mo) in ELISPOT (-) patients (P = 0.009). Black recipients had a greater median HD vintage (55 versus 14 mo in nonblack recipients; P < 0.001). Black recipients with less HD exposure had a low incidence of an ELISPOT (+) test, similar to nonblack recipients. Among variables examined, only HD vintage remained a significant positive correlate with an ELISPOT (+) result (odds ratio per year of HD 1.3; P = 0.003). These data suggest that the risk for developing cross-reactive antidonor T cell immunity increases with longer HD vintage, providing an explanation for the previously observed relationship between increased dialysis exposure and worse posttransplantation outcome. Longer HD vintage may also explain the increased T cell alloreactivity that previously was observed in black kidney recipients.
authors: Division of Nephrology and Hypertension, Case Medical Center, 11100 Euclid Avenue, 1817 Mather, Cleveland, OH 44106, USA. joshua.augustine@uhhospitals.org
authors: Joshua J
authors: JJ
authors: Augustine
authors: Emilio D
authors: ED
authors: Poggio
authors: Michael
authors: M
authors: Clemente
authors: Mark I
authors: MI
authors: Aeder
authors: Kenneth A
authors: KA
authors: Bodziak
authors: James A
authors: JA
authors: Schulak
authors: Peter S
authors: PS
authors: Heeger
authors: Donald E
authors: DE
authors: Hricik
mesh_terms: D000328:Adult; D044383:Black People; D015331:Cohort Studies; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006084:Graft Rejection / Q000208:ethnology / Q000209:etiology* / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular / Q000502:physiology*; D007371:Interferon-gamma / Q000032:analysis; D007518:Isoantibodies / Q000032:analysis; D016030:Kidney Transplantation / Q000276:immunology; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis / Q000009:adverse effects; D012307:Risk Factors; D013997:Time Factors; D014019:Tissue Donors; D014180:Transplantation
chemical_list: D007518:Isoantibodies; D007371:Interferon-gamma