title: A filamentous molecular chaperone of the prefoldin family from the deep-sea hyperthermophile Methanocaldococcus jannaschii.
abstract: Prefoldin is a molecular chaperone found in the domains eukarya and archaea that acts in conjunction with Group II chaperonin to correctly fold other nascent proteins. Previously, our group identified a putative single subunit of prefoldin, gamma PFD, that was up-regulated in response to heat stress in the hyperthermophilic archaeon Methanocaldococcus jannaschii. In order to characterize this protein, we subcloned and expressed it and the other two prefoldin subunits from M. jannaschii, alpha and beta PFD, into Eschericia coli and characterized the proteins. Whereas alpha and beta PFD readily assembled into the expected hexamer, gamma PFD would not assemble with either protein. Instead, gamma PFD forms long filaments of defined dimensions measuring 8.5 nm x 1.7-3.5 nm and lengths exceeding 1 microm. Filamentous gamma PFD acts as a molecular chaperone through in vitro assays, in a manner comparable to PFD. A possible molecular model for filament assembly is discussed.
authors: Department of Chemical Engineering, University of California, Berkeley, Berkeley, California 94720, USA.
authors: Timothy A
authors: TA
authors: Whitehead
authors: Boonchai B
authors: BB
authors: Boonyaratanakornkit
authors: Volker
authors: V
authors: Höllrigl
authors: Douglas S
authors: DS
authors: Clark
mesh_terms: D001483:Base Sequence; D002850:Chromatography, Gel; D002942:Circular Dichroism; D018360:Crystallography, X-Ray; D017931:DNA Primers; D017017:Methanococcus / Q000737:chemistry*; D008958:Models, Molecular; D018832:Molecular Chaperones / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D017931:DNA Primers; D018832:Molecular Chaperones; C112806:prefoldin