title: Stimulation of MAP kinase pathways after maternal IL-1beta exposure induces fetal lung fluid absorption in guinea pigs.
abstract: BACKGROUND
  We tested the hypothesis that maternal interleukin-1beta (IL-1beta) pretreatment and induction of fetal cortisol synthesis activates MAP kinases and thereby affects lung fluid absorption in preterm guinea pigs.
  
  
  METHODS
  IL-1beta was administered subcutaneously daily to timed-pregnant guinea pigs for three days. Fetuses were obtained by abdominal hysterotomy and instilled with isosmolar 5% albumin into the lungs and lung fluid movement was measured over 1 h by mass balance. MAP kinase expression was measured by western blot.
  
  
  RESULTS
  Lung fluid absorption was induced at 61 days (D) gestation and stimulated at 68D gestation by IL-1beta. Maternal IL-1beta pretreatment upregulated ERK and upstream MEK expression at both 61 and 68D gestation, albeit being much more pronounced at 61D gestation. U0126 instillation completely blocked IL-1beta-induced lung fluid absorption as well as IL-1beta-induced/stimulated ERK expression. Cortisol synthesis inhibition by metyrapone attenuated ERK expression and lung fluid absorption in IL-1beta-pretreated fetal lungs. JNK expression after maternal IL-1beta pretreatment remained unaffected at either gestation age.
  
  
  CONCLUSIONS
  These data implicate the ERK MAP kinase pathway as being important for IL-1beta induction/stimulation of lung fluid absorption in fetal guinea pigs.
authors: Department of Physiology and Pharmacology, Northeastern Ohio Universities College of Medicine, Rootstown, OH 44272-0095, USA. bhatta.reshma@gmail.com
authors: Reshma
authors: R
authors: Bhattacharjee
authors: Tianbo
authors: T
authors: Li
authors: Shyny
authors: S
authors: Koshy
authors: LaMonta L
authors: LL
authors: Beard
authors: Kapil
authors: K
authors: Sharma
authors: Ethan P
authors: EP
authors: Carter
authors: Chrystelle
authors: C
authors: Garat
authors: Hans G
authors: HG
authors: Folkesson
mesh_terms: D000042:Absorption / Q000187:drug effects; D000818:Animals; D001826:Body Fluids / Q000378:metabolism*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005260:Female; D005333:Fetus / Q000378:metabolism; D005865:Gestational Age; D006168:Guinea Pigs; D006854:Hydrocortisone / Q000378:metabolism*; D007279:Injections, Subcutaneous; D053583:Interleukin-1beta / Q000008:administration & dosage / Q000494:pharmacology*; D008168:Lung / Q000196:embryology*; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D051381:Rats; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D053583:Interleukin-1beta; D011994:Recombinant Proteins; D048049:Extracellular Signal-Regulated MAP Kinases; D020928:Mitogen-Activated Protein Kinases; D020929:Mitogen-Activated Protein Kinase Kinases; D006854:Hydrocortisone