[The random amplified polymorphic DNA identification of 9 Taenia saginata isolates from four provinces].
OBJECTIVE
To make molecular identification for 9 isolates of Taenia saginata from 4 provinces.


METHODS
Genomic DNA was extracted from the segments of adult tapeworms collected from Taoyuan of Taiwan (TW1), Duyun of Guizhou (DY1, DY2), Congjiang of Guizhou (CJ1, CJ2, CJ3, CJ4), Dali of Yunnan (DL1) and Wushi of Xinjiang (XJ1) respectively. PCRs were carried out with 13 random primers. A phylogenetic tree of different geographical strains was constructed.


RESULTS
331 DNA fragments were amplified. The number of DNA fragments amplified by single primer was between 3 and 28. The average number of amplified DNA fragments by the 13 primers was 14.15. The average number of fragments from the 9 isolates of T. saginata was 14.08. Phylogenetic tree revealed that there were two branches in the tree, DY1, DY2, DL1 and TW1 occupied one branch, while CJ1, CJ2, CJ3, CJ4 and XJ1 occupied the other one.


CONCLUSIONS
By the RAPD analysis, the isolates DY1, DY2, DL1 and TW1 belong to Taenia saginata asiatica, and the isolates CJ1, CJ2, CJ3, CJ4 and XJ1 belong to T. saginata saginata.
Zhang
Ke
K
Department of Parasitology, Guiyang Medical College, Guiyang 550004, China.
Yang
Ming
M
Bao
Huai-En
HE
D000818:Animals; D002681:China; D016000:Cluster Analysis; D003551:Cysticercosis / Q000469:parasitology*; D017931:DNA Primers; D018509:DNA, Helminth / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D041202:Taenia saginata / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
D017931:DNA Primers; D018509:DNA, Helminth