title: A cloning and expression analysis of pregnancy-associated glycoproteins expressed in trophoblasts of the white-tail deer placenta.
abstract: The pregnancy-associated glycoproteins (PAGs) are placental proteins that have been cloned from swine, sheep, goats, and cattle, but never from animals within the Cervidae family. The goal of this work was to characterize PAGs in white-tailed deer. Placenta and uterine tissues were collected from pregnant does at days 85 and 90 of pregnancy. RNA from cotyledons was used to amplify deer PAGs by RT-PCR. Ten distinct cDNAs were cloned and sequenced. Some normally conserved amino acids comprising the catalytic site were found to be altered in deer PAGs 4, 5, and 8; another PAG, (PAG-9) was a splice variant that lacked exon 7. In each case, these mutations would likely preclude proteolytic activity for these proteins. A phylogenetic analysis revealed that most of the deer PAGs fell within the ancient PAG grouping. The remainder fell within the more modern (BNC-specific) PAG group. Western blotting was performed with anti-PAG antibodies and this analysis revealed that deer PAGs comprise a heterogeneous group based on different antigenicities and electrophoretic mobilities. Immunohistochemistry and in situ hybridization revealed some unique localization patterns of PAGs in the deer placentome compared to those in other ruminants. Most notably, deer PAGs 4 and 5, which according to the phylogeny, are "ancient PAGs," were expected to be present in all trophoblasts; instead, they were localized to the BNC. Although many of the PAGs identified here are very similar to those in Bovidae, some are clearly distinct in their expression pattern and probably possess functional roles unique to cervid reproduction.
authors: Department of Animal Sciences, University of Missouri-Columbia, Columbia, Missouri 65211-5300, USA.
authors: Gretchen A
authors: GA
authors: Brandt
authors: Tina E
authors: TE
authors: Parks
authors: Gary
authors: G
authors: Killian
authors: Alan D
authors: AD
authors: Ealy
authors: Jonathan A
authors: JA
authors: Green
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D003670:Deer / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006023:Glycoproteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D010802:Phylogeny; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D014327:Trophoblasts / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; D011257:Pregnancy Proteins