Liquid chromatography/electrospray ionization tandem mass spectrometry profiling of compounds from the infusion of Byrsonima fagifolia Niedenzu.
A rapid analytical approach suitable to achieve a comprehensive characterization of the compounds present in the infusion prepared from the leaves of Byrsonima fagifolia Niedenzu (Malpighiaceae), a Brazilian plant used as an infusion to treat gastric disorders, was developed. The method was based on high-performance liquid chromatography coupled to electrospray negative ionisation multistage ion trap mass spectrometry (HPLC/ESI-ITMSn). The main ions in the ESI-ITMS spectra were attributed to a quinic acid core containing from one to five galloyl units. Quercetin derivatives containing one and two sugar moieties as well as galloyl esterification were also detected. These results indicated that HPLC/ESI-ITMSn is easily applicable to infusions of this plant and allows the rapid and direct identification of these compounds in crude plant extracts.
Sannomiya
Miriam
M
Departamento de Química Orgânica, Instituto de Química de Araraquara, São Paulo State University-UNESP, Rua Francisco Degni s/n, Bairro Quitandinha, CEP 14800-900 Araraquara, SP, Brazil.
dos Santos
Lourdes Campaner
LC
Carbone
Virginia
V
Napolitano
Assunta
A
Piacente
Sonia
S
Pizza
Cosimo
C
Souza-Brito
Alba R M
AR
Vilegas
Wagner
W
D001938:Brazil; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005707:Gallic Acid / Q000032:analysis; D029581:Malpighiaceae / Q000737:chemistry*; D008519:Medicine, Traditional; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011794:Quercetin / Q000031:analogs & derivatives / Q000032:analysis; D011801:Quinic Acid / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry
D010936:Plant Extracts; D011801:Quinic Acid; D005707:Gallic Acid; D011794:Quercetin