Assessment of oxidative stress due to exposure to pesticides in plasma and urine of traditional limited-resource farm workers: formation of the DNA-adduct 8-hydroxy-2-deoxy-guanosine (8-OHdG).
The objective of this study was to assess the risk of genotoxicity caused due to oxidative stress using plasma and urinary levels of 8-hydroxy-2-deoxyguanosine (8-OHdG) in farm workers for six months during a growing season. Blood and urine samples were collected once a month for six months (June to November 2003) from farm workers (n = 15) and urban unexposed controls (n = 10). Plasma and urinary levels of 8-OHdG were evaluated by Enzyme Linked Immunosorbent Assay (ELISA) technique. There was no significant difference in the urinary levels of 8-OHdG between the farm workers and the control group, but there was an approximately four-fold increase in mean values of plasma 8-OHdG levels in the farm worker group (P < or = 0.05).
Tope
Avinash M
AM
Nutrition and Health Program, Kentucky State University, Frankfort, Kentucky 40601, USA. avinash.tope@kysu.edu
Panemangalore
Myna
M
D000080242:8-Hydroxy-2'-Deoxyguanosine; D015415:Biomarkers / Q000097:blood / Q000652:urine; D018736:DNA Adducts; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine; D004784:Environmental Monitoring; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure; D018384:Oxidative Stress / Q000187:drug effects; D010575:Pesticides / Q000009:adverse effects*; D012307:Risk Factors; D012621:Seasons; D014171:Transients and Migrants
D015415:Biomarkers; D018736:DNA Adducts; D010575:Pesticides; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine