The role of pannexin 1 hemichannels in ATP release and cell-cell communication in mouse taste buds.
ATP has been shown to be a taste bud afferent transmitter, but the cells responsible for, and the mechanism of, its release have not been identified. Using CHO cells expressing high-affinity neurotransmitter receptors as biosensors, we show that gustatory stimuli cause receptor cells to secrete ATP through pannexin 1 hemichannels in mouse taste buds. ATP further stimulates other taste cells to release a second transmitter, serotonin. These results provide a mechanism to link intracellular Ca(2+) release during taste transduction to secretion of afferent transmitter, ATP, from receptor cells. They also indicate a route for cell-cell communication and signal processing within the taste bud.
Huang
Yi-Jen
YJ
Department of Physiology and Biophysics, Miller School of Medicine, University of Miami, Miami, FL 33136, USA.
Maruyama
Yutaka
Y
Dvoryanchikov
Gennady
G
Pereira
Elizabeth
E
Chaudhari
Nirupa
N
Roper
Stephen D
SD
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D016466:CHO Cells; D002118:Calcium / Q000378:metabolism; D002450:Cell Communication / Q000502:physiology*; D017630:Connexins; D006224:Cricetinae; D003412:Cricetulus; D017403:In Situ Hybridization; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012701:Serotonin / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D013650:Taste Buds / Q000502:physiology*
D017630:Connexins; D009419:Nerve Tissue Proteins; C503631:Panx1 protein, mouse; D012701:Serotonin; D000255:Adenosine Triphosphate; D002118:Calcium