Sensitization of anoxic recombination-deficient mutants of escherichia coli K12 to X-rays by triacetoneamine N-oxyl.
The radiosensitizing effect of triacetoneamine N-oxyl (TAN) on anoxic suspensions of two x-ray-sensitive mutants of E. coli K12 was investigated. With one mutant, AB2463 recA13, TAN increased the sensitivity (based on the dose required to give 1% surviving fraction) of anoxic cells by a factor of 3.9, while oxygen increased the sensitivity by a factor of only 1.9. On the other hand, on the same basis, TAN was only about one-half as effective as oxygen in sensitizing the mutant AB3058 rec-22. The results are interpreted in terms of the relative ability of the cells to recover from radiation damage produced in the presence of TAN and in the presence of oxygen.
Emmerson
P T
PT
Department of Radiology, Yale University School of Medicine, New Haven, Connecticut, USA.
D015687:Cell Hypoxia / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D048168:Escherichia coli K12 / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects*; D009154:Mutation; D011829:Radiation Dosage; D011836:Radiation Tolerance / Q000187:drug effects / Q000502:physiology*; D011838:Radiation-Sensitizing Agents / Q000008:administration & dosage; D011995:Recombination, Genetic; D014216:Triacetoneamine-N-Oxyl / Q000008:administration & dosage*; D014965:X-Rays
D011838:Radiation-Sensitizing Agents; D014216:Triacetoneamine-N-Oxyl