title: Mediating delta-opioid-initiated heart protection via the beta2-adrenergic receptor: role of the intrinsic cardiac adrenergic cell.
abstract: Stimulation of cardiac beta(2)-adrenergic receptor (beta(2)-AR) or delta-opioid receptor (DOR) exerts a similar degree of cardioprotection against myocardial ischemia in experimental models. We hypothesized that delta-opioid-initiated cardioprotection is mediated by the intrinsic cardiac adrenergic (ICA) cell via enhanced epinephrine release. Using immunohistochemical and in situ hybridization methods, we detected in situ tyrosine hydroxylase (TH) mRNA and TH immunoreactivity that was colocalized with DOR immunoreactivity in ICA cells in human and rat hearts. Western blot analysis detected DOR protein in ICA cells isolated from rat ventricular myocytes. The physiology of DOR expression was examined by determining changes of cytosolic Ca(2+) concentration ([Ca(2+)](i)) transients in isolated rat ICA cells using fluorescence spectrophotometry. Exposing the selective delta-opioid agonist D-[Pen(2,5)]enkephalin (DPDPE) to ICA cells increased [Ca(2+)](i) transients in a concentration-dependent manner. Such an effect was abolished by the Ca(2+) channel blocker nifedipine. HPLC-electrochemical detection demonstrated a 2.4-fold increase in epinephrine release from ICA cells following DPDPE application. The significance of the ICA cell and its epinephrine release in delta-opioid-initiated cardioprotection was demonstrated in the rat myocardial infarction model and ICA cell-ventricular myocyte coculture. DPDPE administered before coronary artery occlusion or simulated ischemia-reperfusion reduced left ventricular infarct size by 54 +/- 15% or myocyte death by 26 +/- 4%, respectively. beta(2)-AR blockade markedly attenuated delta-opioid-initiated infarct size-limiting effect and abolished delta-opioid-initiated myocyte survival protection in rat ICA cell-myocyte coculture. Furthermore, delta-opioid agonist exerted no myocyte survival protection in the absence of cocultured ICA cells during ischemia-reperfusion. We conclude that delta-opioid-initiated myocardial infarct size reduction is primarily mediated via endogenous epinephrine/beta(2)-AR signaling pathway as a result of ICA cell activation.
authors: Deptartment of Internal Medicine, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555-0553, USA. mihuang@utmb.edu
authors: Ming-He
authors: MH
authors: Huang
authors: Hui-Qun
authors: HQ
authors: Wang
authors: William R
authors: WR
authors: Roeske
authors: Yochai
authors: Y
authors: Birnbaum
authors: Yewen
authors: Y
authors: Wu
authors: Ning-Ping
authors: NP
authors: Yang
authors: Yu
authors: Y
authors: Lin
authors: Yumei
authors: Y
authors: Ye
authors: David J
authors: DJ
authors: McAdoo
authors: Michael G
authors: MG
authors: Hughes
authors: Scott D
authors: SD
authors: Lick
authors: Paul J
authors: PJ
authors: Boor
authors: Charles Y
authors: CY
authors: Lui
authors: Barry F
authors: BF
authors: Uretsky
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects; D002316:Cardiotonic Agents / Q000008:administration & dosage; D002478:Cells, Cultured; D020881:Enkephalin, D-Penicillamine (2,5)- / Q000008:administration & dosage; D006801:Humans; D015428:Myocardial Reperfusion Injury / Q000378:metabolism* / Q000517:prevention & control; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018343:Receptors, Adrenergic, beta-2 / Q000378:metabolism*; D017465:Receptors, Opioid, delta / Q000819:agonists / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D002316:Cardiotonic Agents; D018343:Receptors, Adrenergic, beta-2; D017465:Receptors, Opioid, delta; D020881:Enkephalin, D-Penicillamine (2,5)-; D002118:Calcium