MgAtr7, a new type of ABC transporter from Mycosphaerella graminicola involved in iron homeostasis.
The ABC transporter-encoding gene MgAtr7 from the wheat pathogen Mycosphaerella graminicola was cloned based upon its high homology to ABC transporters involved in azole-fungicide sensitivity. Genomic and cDNA sequences indicated that the N-terminus of this ABC transporter contains a motif characteristic for a dityrosine/pyoverdine biosynthesis protein. This makes MgAtr7 the first member of a new class of fungal ABC transporters harboring both a transporter and a biosynthetic moiety. A homologue of MgAtr7 containing the same biosynthetic moiety was only found in the Fusarium graminearum genome and not in any other fungal genome examined so far. The gene structure of both orthologous transporters is highly conserved and the genomic area surrounding the ABC transporter exhibits micro-synteny between M. graminicola and F. graminearum. Functional analyses revealed that MgAtr7 is neither required for virulence nor involved in fungicide sensitivity but indicated a role in maintenance of iron homeostasis.
Zwiers
Lute-Harm
LH
Laboratory of Phytopathology, Wageningen University, P.O. Box 8025, 6700 EE Wageningen, The Netherlands. Zwiers@cbs.knaw.nl
Roohparvar
Ramin
R
de Waard
Maarten A
MA
D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D001203:Ascomycota / Q000235:genetics / Q000502:physiology*; D025141:Drug Resistance, Fungal / Q000235:genetics / Q000502:physiology; D005656:Fungal Proteins / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D006706:Homeostasis / Q000502:physiology*; D007501:Iron / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology
D018528:ATP-Binding Cassette Transporters; D005656:Fungal Proteins; D007501:Iron