title: Interspecies comparative genomic hybridization (I-CGH): a new twist to study animal tumor models.
abstract: Animal models of human diseases are widely used to address questions of tumor development. Selection of a particular animal model depends upon a variety of factors, among them: animal cost, species lifespan and hardiness; availability of biomolecular and genetic tools for that species; and evolutionary distance from humans. In spite of the growth in genomic data in the past several years, many animal models cannot yet be studied extensively due to gaps in genetic mapping, sequencing and functional analyses. Thus, alternative molecular genetic approaches are needed. We have designed an interspecies comparative genomic hybridization approach to analyze genetic changes in radiation-induced brain tumors in the non-human primate, Macaca mulatta. Using homologies between the primate and human genomes, we adapted widely-available CGH techniques to generate cytogenetic profiles of malignant gliomas in four monkey tumors. Losses and gains were projected onto the corresponding homologous chromosomal regions in the human genome, thus directly translating the status of the monkey gliomas into human gene content. This represents a novel method of comparative interspecies cytogenetic mapping that permits simultaneous analysis of genomic imbalances of unknown sequences in disparate species and correlation with potential or known human disease-related genes.
authors: Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, USA.
authors: Sivakumar
authors: S
authors: Jaikumar
authors: Zhengping
authors: Z
authors: Zhuang
authors: Poonam
authors: P
authors: Mannan
authors: Alexander O
authors: AO
authors: Vortmeyer
authors: Makoto
authors: M
authors: Furuta
authors: Rob
authors: R
authors: Dickerman
authors: Julia
authors: J
authors: Bedanova
authors: Russell R
authors: RR
authors: Lonser
authors: Stuart
authors: S
authors: Walbridge
authors: Robert J
authors: RJ
authors: Weil
authors: Victor V
authors: VV
authors: Lobanenkov
authors: Edward H
authors: EH
authors: Oldfield
authors: Svetlana D
authors: SD
authors: Pack
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000235:genetics*; D004195:Disease Models, Animal; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D015894:Genome, Human / Q000235:genetics; D042822:Genomic Instability / Q000235:genetics; D015698:Genomic Library; D005910:Glioma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D008253:Macaca mulatta / Q000235:genetics*; D008297:Male; D008967:Molecular Biology / Q000379:methods*; D009693:Nucleic Acid Hybridization / Q000235:genetics*; D013045:Species Specificity