Transgenic malaria-resistant mosquitoes have a fitness advantage when feeding on Plasmodium-infected blood.
The introduction of genes that impair Plasmodium development into mosquito populations is a strategy being considered for malaria control. The effect of the transgene on mosquito fitness is a crucial parameter influencing the success of this approach. We have previously shown that anopheline mosquitoes expressing the SM1 peptide in the midgut lumen are impaired for transmission of Plasmodium berghei. Moreover, the transgenic mosquitoes had no noticeable fitness load compared with nontransgenic mosquitoes when fed on noninfected mice. Here we show that when fed on mice infected with P. berghei, these transgenic mosquitoes are more fit (higher fecundity and lower mortality) than sibling nontransgenic mosquitoes. In cage experiments, transgenic mosquitoes gradually replaced nontransgenics when mosquitoes were maintained on mice infected with gametocyte-producing parasites (strain ANKA 2.34) but not when maintained on mice infected with gametocyte-deficient parasites (strain ANKA 2.33). These findings suggest that when feeding on Plasmodium-infected blood, transgenic malaria-resistant mosquitoes have a selective advantage over nontransgenic mosquitoes. This fitness advantage has important implications for devising malaria control strategies by means of genetic modification of mosquitoes.
Marrelli
Mauro T
MT
Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health and Malaria Research Institute, The Johns Hopkins University, Baltimore, MD 21205, USA.
Li
Chaoyang
C
Rasgon
Jason L
JL
Jacobs-Lorena
Marcelo
M
D000821:Animal Feed; D000818:Animals; D030801:Animals, Genetically Modified; D001769:Blood / Q000469:parasitology*; D009033:Culicidae / Q000502:physiology*; D004195:Disease Models, Animal; D006790:Host-Parasite Interactions; D007303:Insect Vectors; D008288:Malaria / Q000401:mortality / Q000473:pathology; D051379:Mice; D008957:Models, Genetic; D010961:Plasmodium / Q000378:metabolism*; D010962:Plasmodium berghei / Q000378:metabolism; D019076:Transgenes