title: Hydroxymethylfurfural: an enemy or a friendly xenobiotic? A bioanalytical approach.
abstract: Hydroxymethylfurfural (HMF), a well-known heterocyclic Maillard reaction product, has often been studied for its potential toxic, mutagenic, and carcinogenic effects. Recent clinical studies, however, have strongly suggested that HMF might have exciting antitumor potential. We report on the development and validation of a bioanalytical assay for HMF that could be suitable as a basis for pharmacokinetic models in cancer patients. Two strategies were tested, i.e., direct and indirect methodologies. A direct isocratic LC determination at 283 nm was designed. Two indirect attempts involved derivatization coupled to HPLC-UV. It was possible to resolve the stereoisomers of the HMF derivative, and factors influencing their equilibrium ratio are discussed. HMF was extracted from the biomatrix by solid-phase extraction using different cartridges. A comparative study was made of the implemented methods as well as the extraction protocols. Both indirect assays proved to be more sensitive and were used to assess HMF quantitatively in human plasma. However, the newly introduced derivatization conditions led to the highest sensitivity with a LOD (S/N ratio = 3) of at least 2 pmol analyte on column. The assay selectivity was satisfactory in pre- and post-dose real samples. The mean recoveries of the assays were 79% and 89%, with acceptable accuracies and reproducibilities. Figure Schematic representation of hydroxymethylfurfural (HMF) in human plasma.
authors: Institute of Pharmaceutical Sciences, Pharmaceutical Chemistry, University of Graz, Schubertstrasse 1, 8010 Graz, Austria.
authors: K
authors: K
authors: Michail
authors: V
authors: V
authors: Matzi
authors: A
authors: A
authors: Maier
authors: R
authors: R
authors: Herwig
authors: J
authors: J
authors: Greilberger
authors: H
authors: H
authors: Juan
authors: O
authors: O
authors: Kunert
authors: R
authors: R
authors: Wintersteiger
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000097:blood; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: C008046:5-hydroxymethylfurfural; D005662:Furaldehyde