title: Immune response against polyester implants is influenced by the coating substances.
abstract: The aim of this study was to evaluate the influence of the coating of polymer implants upon the individual humoral immune response to the polymer matrix. Intramuscular implantation and explantation of samples from three different polyester vascular prostheses coated with collagen, gelatin, or human serum albumin was performed in LEW.1A rats and subsequently compared to sham operated control animals. Antibodies in serum samples were detected by means of enzyme immunoassays employing particles of pure polyester and the respective prosthesis, or solid phase bound coating substances as targets. In contrast to the controls, all animals with implants demonstrated a high antipolyester antibody response with a broad individual variability graduated according to the prosthesis coatings: gelatin > albumin > collagen. This was further significantly increased after the second implantation/first explantation and declined following the last explantation. Only animals with albumin-coated implants revealed specific antibodies to the coating as well as the strongest overall immunological reaction against the prosthesis already on day 8. Specificity of polymer antibodies was demonstrated by competitive inhibition of median antibody binding. Our results showed a specific immune reaction as a result of the applied polymer, which varied due to the surface-coating and individual factors.
authors: Department of Surgery, University of Greifswald, Greifswald D-17487, Germany.
authors: Lutz
authors: L
authors: Wilhelm
authors: Roland
authors: R
authors: Zippel
authors: Thomas
authors: T
authors: von Woedtke
authors: Heidrun
authors: H
authors: Kenk
authors: Andreas
authors: A
authors: Hoene
authors: Maciej
authors: M
authors: Patrzyk
authors: Michael
authors: M
authors: Schlosser
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D000917:Antibody Formation / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D001667:Binding, Competitive; D020099:Coated Materials, Biocompatible / Q000378:metabolism*; D003094:Collagen / Q000276:immunology*; D005780:Gelatin / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D011091:Polyesters / Q000378:metabolism*; D019736:Prostheses and Implants; D051381:Rats; D012709:Serum Albumin / Q000276:immunology*; D013997:Time Factors
chemical_list: D000906:Antibodies; D020099:Coated Materials, Biocompatible; D007074:Immunoglobulin G; D011091:Polyesters; D012709:Serum Albumin; D005780:Gelatin; D003094:Collagen