title: Hepatoprotective effect of Vitis vinifera L. leaves on carbon tetrachloride-induced acute liver damage in rats.
abstract: The hepatoprotective effect of ethanolic extract and its four different fractions (CHCl(3), EtOAc, n-BuOH, and remaining water fraction) of Vitis vinifera L. leaves was investigated against carbon tetrachloride (CCl(4))-induced acute hepatotoxicity in rats. The ethanolic extract was found active at 125mg/kg dose (per os). The ethanolic extract was fractionated through successive solvent-solvent extractions and the n-BuOH fraction in 83mg/kg dose possessed remarkable antioxidant and hepatoprotective activities. Liver damage was assessed by using biochemical parameters (plasma and liver tissue MDA [malondialdehyde], transaminase enzyme levels in plasma [AST-aspartate transaminase, ALT-alanine transferase] and liver GSH [glutathione] levels). Additionally, the pathological changes in liver were evaluated by histopathological studies. Legalon 70 Protect was used as standard natural originated drug.
authors: Gazi University, Faculty of Pharmacy, Department of Pharmacognosy, Hipodrom 06330, Ankara, Turkey. didem@gazi.edu.tr
authors: Didem Deliorman
authors: DD
authors: Orhan
authors: Nilüfer
authors: N
authors: Orhan
authors: Ender
authors: E
authors: Ergun
authors: Fatma
authors: F
authors: Ergun
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D002252:Carbon Tetrachloride Poisoning / Q000473:pathology / Q000517:prevention & control*; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology / Q000517:prevention & control*; D005419:Flavonoids / Q000737:chemistry; D015227:Lipid Peroxidation / Q000187:drug effects; D008111:Liver Function Tests; D008297:Male; D010636:Phenols / Q000737:chemistry; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use; D018515:Plant Leaves; D051381:Rats; D017208:Rats, Wistar; D027843:Vitis
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts