title: Comparison of the measurement of lysosomal hydrolase activity in mycoplasma-contaminated and non-contaminated human fibroblast cultures treated with mycoplasma removal agent.
abstract: OBJECTIVE
  To investigate the effect of both mycoplasma contamination and of its remover (MRA), through human fibroblasts culture over the activity of some lysosomal hydrolases.
  
  
  METHODS
  Activity was measured in contaminated fibroblasts before and after the addition of MRA. Results were compared with the enzymatic activity in control fibroblasts with and without MRA.
  
  
  RESULTS
  Only beta-glucosidase showed no significant alteration in the presence of either mycoplasma or MRA. Total hexosaminidase and beta-galactosidase underwent significant interference in the presence of the mycoplasma and the MRA. The % of hexosaminidase A and arylsulphatase A altered their activity only in the presence of MRA. Beta-glucuronidase changed its activity only in the presence of mycoplasma.
  
  
  CONCLUSIONS
  The fibroblast enzymes behaved differently in the presence of MRA and/or mycoplasma, demonstrating the sensitivity of these hydrolases. Our work suggests that mycoplasma and MRA alter the activity of some lysosomal hydrolases.
authors: Departamento de Bioquímica, ICBS, UFRGS, Porto Alegre, RS, Brazil.
authors: Fernanda Timm Seabra
authors: FT
authors: Souza
authors: Luana
authors: L
authors: Souza Sostruznik
authors: Roberta
authors: R
authors: Casagrande Scolari
authors: Karen Joana
authors: KJ
authors: Maciel de Castro
authors: Roberto
authors: R
authors: Giugliani
authors: Janice Carneiro
authors: JC
authors: Coelho
mesh_terms: D002478:Cells, Cultured; D004789:Enzyme Activation / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects* / Q000201:enzymology / Q000382:microbiology; D005959:Glucosidases / Q000378:metabolism; D005966:Glucuronidase / Q000378:metabolism; D006596:Hexosaminidases / Q000378:metabolism; D006801:Humans; D006867:Hydrolases / Q000378:metabolism*; D008247:Lysosomes / Q000201:enzymology*; D009174:Mycoplasma / Q000187:drug effects* / Q000254:growth & development; D015363:Quinolones / Q000494:pharmacology; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D015363:Quinolones; C074448:mycoplasma removal agent; D006867:Hydrolases; D005959:Glucosidases; D006596:Hexosaminidases; D001616:beta-Galactosidase; D005966:Glucuronidase