Raman sensitivity enhancement for aqueous absorbing sample using Teflon-AF 2400 liquid core optical fibre cell.
The compatibility Teflon-AF 2400 liquid core optical fibre with resonance Raman spectroscopy (RRS-LCOF) was used to detect aqueous biomolecules. The maximum sensitivity enhancement factor for concentrations greater than the detection limit in a conventional cell was 10, and detection limit reduction of about 1000-fold have been achieved for the measurement of aqueous absorbing sample using Teflon-AF 2400 fibre Raman cell compared to the conventional cell. We were able to collect spectra of 2.5 x 10(-9) and 2.5 x 10(-10) M aqueous beta-carotene using 16.2 mW of laser power and 10s integration time. This volume of a 2.5 x 10(-10) M aqueous solution corresponds to only 1.5 fmol or 830 fg of beta-carotene. The results of this preliminary study indicate that RRS-LCOF has potential in bioanalytical and biomedical applications.
Tian
Yanjie
Y
Key Laboratory of Coherent Light, Atomic and Molecular Spectroscopy, Educational Ministry of China, College of Physics, Jilin University, Changchun 130023, PR China.
Zhang
Liuyang
L
Zuo
Jian
J
Li
Zuowei
Z
Gao
Shuqin
S
Lu
Guohui
G
D000042:Absorption; D005336:Fiber Optic Technology / Q000295:instrumentation / Q000379:methods*; D011138:Polytetrafluoroethylene / Q000032:analysis*; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*; D014867:Water / Q000032:analysis*
D014867:Water; D011138:Polytetrafluoroethylene