Immortalization and characterization of porcine ventricular endocardial endothelial cells.
Endocardial endothelial cells (EECs), which form the inner lining of the cavities of the heart, are a distinct cell population whose dysfunction can be critical in pathological conditions of heart. Insights into the role and organization of these cells in pathological states of the heart are limited mainly due to a dearth of experimental models. To date no endocardial endothelial cell line is available. The authors attempted to immortalize porcine ventricular EECs by transfecting the cells with human telomerase reverse transcriptase (hTERT). EECs immortalized by ectopic expression of hTERT exhibit phenotypic and functional characteristics similar to primary EECs. The EE cell line could be useful for the study of mechanisms involved in the interaction of EECs with the underlying myocardium and cardiac interstitium and as useful tools in understanding their role in diseased states of heart.
Kuruvilla
Leena
L
Division of Cellular and Molecular Cardiology, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, India.
T R
Santhoshkumar
S
Kartha
Chandrasekharan Cheranellore
CC
D000818:Animals; D015153:Blotting, Western; D002455:Cell Division / Q000502:physiology; D002461:Cell Line, Transformed; D004699:Endocardium / Q000166:cytology*; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology*; D016650:Fluorescein-5-isothiocyanate; D019085:Fluorescent Antibody Technique, Direct; D005456:Fluorescent Dyes; D006352:Heart Ventricles / Q000166:cytology*; D006801:Humans; D007150:Immunohistochemistry; D007700:Kinetics; D009573:Nitrites / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D019098:Telomerase / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
D005456:Fluorescent Dyes; D009573:Nitrites; C509186:TERT protein, human; D019098:Telomerase; D016650:Fluorescein-5-isothiocyanate