[Change in gene expression of inflammation-related genes induced in multiple organ dysfunction syndrome induced by infection of injuries in rat].
OBJECTIVE
To study the changes in inflammation-related gene expression in liver tissue during the course of multiple organ dysfunction syndrome (MODS) induced by infection of injuries and its implication.


METHODS
The rats model with MODS following trauma and infection was reproduced in rat. Liver tissue was harvested. The differences of gene expressions between the simple trauma group and MODS group were detected by means of cDNA microarray.


RESULTS
Comparison between the two groups, differentially expressed genes included enhanced expression of genes related both of tissue damage and repair. There was also up-regulation of expression of both inflammation-related and anti-inflammation related genes. A few genes appeared down-regulated. The differences of expression extent were significant. There were up-regulation of some genes related to apoptosis and fibrosis.


CONCLUSIONS
Differential expressions of genes in the liver tissue include both that related to the inflammation and anti-inflammation, with down-regulation and up-regulation at the same time. There is a difference in the intensity. There is also an expression of genes related to intrinsic protection, as manifested by co-existence of systemic inflammatory response syndrome (SIRS) and compensation anti-inflammatory response syndrome (CARS) under the condition of MODS. There is an imbalance in inflammatory reaction. The simultaneous up-regulation of the tissue damage and repair related genes suggests that cellular injury is accompanied by repair in the organs during the course of MODS.
Ban
Yu
Y
Department of Emergency, General Hospital of PLA, Beijing 100853, China.
Shen
Hong
H
Li
Tan-shi
TS
D000818:Animals; D004195:Disease Models, Animal; D015536:Down-Regulation; D020869:Gene Expression Profiling; D007249:Inflammation / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D009102:Multiple Organ Failure / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation; D014946:Wound Infection / Q000150:complications