Genes expression analyses of sea-island cotton (Gossypium barbadense L.) during fiber development.
Sea-island cotton (Gossypium barbadense L.) is one of the most valuable cotton species due to its silkiness, luster, long staples, and high strength, but its fiber development mechanism has not been surveyed comprehensively. We constructed a normalized fiber cDNA library (from -2 to 25 dpa) of G. barbadense cv. Pima 3-79 (the genetic standard line) by saturation hybridization with genomic DNA. We screened Pima 3-79 fiber RNA from five developmental stages using a cDNA array including 9,126 plasmids randomly selected from the library, and we selected and sequenced 929 clones that had different signal intensities between any two stages. The 887 high-quality expressed sequence tags obtained were assembled into 645 consensus sequences (582 singletons and 63 contigs), of which 455 were assigned to functional categories using gene ontology. Almost 50% of binned genes belonged to metabolism functional categories. Based on subarray analysis of the 887 high-quality expressed sequence tags with 0-, 5-, 10-, 15-, and 20-dpa RNA of Pima 3-79 fibers and a mixture of RNA of nonfiber tissues, seven types of expression profiles were elucidated. Furthermore our results showed that phytohormones may play an important role in the fiber development.
Tu
Li-Li
LL
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
Zhang
Xian-Long
XL
Liang
Shao-Guang
SG
Liu
Di-Qiu
DQ
Zhu
Long-Fu
LF
Zeng
Fan-Chang
FC
Nie
Yi-Chun
YC
Guo
Xiao-Ping
XP
Deng
Feng-Lin
FL
Tan
Jia-Fu
JF
Xu
Li
L
D032561:Cotton Fiber; D018744:DNA, Plant / Q000235:genetics; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D003368:Gossypium / Q000235:genetics* / Q000254:growth & development*; D008969:Molecular Sequence Data
D018744:DNA, Plant