Microvillar bleb formation in primate hepatocytes irradiated with 32-meV protons.
Eight rhesus monkeys (Macaca mulatta) were whole-body irradiated with 6000 rads of 32-MeV protons. Animals were killed at several time intervals after exposure. Tissue blocks were fixed in glutaraldehyde, post-osmicated, and then embedded in Maraglas-D.E.R. 732 resin. Observation of ultrathin sections by electron microscopy revealed balloon-like enlargements of distal portions of hepatocytic microvilli (blebs) which were less dense than the proximal portion of microvilli. The blebs ranged widely in size; some were contained within the space of Disse, whereas many protruded into the sinusoidal lumen. In a few micrographs the sinusoidal lumen as filled with bleb-like structures. Such bleb formation suggests a possible mechanistic explanation for hepatic congestion following high levels of irradiation.
Ghidoni
J J
JJ
Laboratory of Experimental Pathology, Department of Pathology, Baylor University College of Medicine, Houston, Texas, USA.
Thomas
H
H
D000818:Animals; D001768:Blister / Q000209:etiology* / Q000473:pathology*; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D022781:Hepatocytes / Q000473:pathology* / Q000528:radiation effects*; D008099:Liver / Q000473:pathology* / Q000528:radiation effects*; D008253:Macaca mulatta; D008871:Microvilli / Q000473:pathology / Q000528:radiation effects; D011522:Protons / Q000009:adverse effects*; D011829:Radiation Dosage; D014916:Whole-Body Irradiation
D011522:Protons