title: Comparative analysis of epicuticular lipid profiles of sympatric and allopatric field populations of Anopheles gambiae s.s. molecular forms and An. arabiensis from Burkina Faso (West Africa).
abstract: We analysed by gas chromatography-mass spectrometry (GC-MS) and Gas Chromatography-Flame Ionization Detector (GC-FID) the epicuticular lipid profiles of field females of the major Afro-tropical malaria vector, Anopheles gambiae. The samples were collected in three villages in Burkina Faso (West Africa), where An. gambiae M and S molecular forms and An. arabiensis live sympatrically. The aim was to compare the cuticular hydrocarbon (CHC) composition of individual field specimens of these three taxa, to highlight possible differences among them. All the samples analysed by GC-MS (55 individuals and eight pools) were characterized by the same 48 CHCs and 10 oxygenated compounds. The 19 most abundant CHCs were quantified in 174 specimens by GC-FID: quantitative intra-taxon differences were found between allopatric populations of both An. arabiensis and S-form. Inter-taxa quantitative differences in the relative abundances of some hydrocarbons between pairs of sympatric taxa were also found, which appear to be mainly linked to local situations, with the possible exception of diMeC(35) between An. arabiensis and S-form. Moreover, MeC(29) shows some degree of differentiation between S- and M-form in all three villages. Possible causes of these differences are discussed.
authors: Sezione di Parassitologia, Dipartimento di Scienze di Sanità Pubblica, Università di Roma "La Sapienza", Piazzale Aldo Moro 5, 00185, Rome, Italy.
authors: B
authors: B
authors: Caputo
authors: F R
authors: FR
authors: Dani
authors: G L
authors: GL
authors: Horne
authors: S
authors: S
authors: N'Fale
authors: A
authors: A
authors: Diabate
authors: S
authors: S
authors: Turillazzi
authors: M
authors: M
authors: Coluzzi
authors: C
authors: C
authors: Costantini
authors: A A
authors: AA
authors: Priestman
authors: V
authors: V
authors: Petrarca
authors: A
authors: A
authors: della Torre
mesh_terms: D000818:Animals; D000852:Anopheles / Q000737:chemistry* / Q000235:genetics; D005260:Female; D049810:Genetic Speciation; D006838:Hydrocarbons / Q000032:analysis*; D013045:Species Specificity
chemical_list: D006838:Hydrocarbons