Determination of lysozyme at the nanogram level by a resonance light-scattering technique with functionalized CdTe nanoparticles.
Nanoparticles of cadmium telluride coated with mercaptoacetic acid were prepared in the water phase. Further, an assay of lysozyme with a sensitivity at the nanogram level is proposed. At pH 7.28, lysozyme with positive charges can interact with CdTe nanoparticles. The resonance light-scattering (RLS) signals of functionalized nano-CdTe were greatly enhanced by lysozyme in the region of 300-600 nm, characterized with peaks located at 367, 470 and 533 nm. A linear relationship could be established between the enhanced RLS intensity and the lysozyme concentration in the range of 0.06-4.0 microg mL-1. The limit of detection was 9.5 ng mL-1. The contents of lysozyme were determined with recoveries of 95.6-104.8% and RSD of 1.5-2.3%, respectively. This method is sensitive, rapid, accurate and simple, and provides a new and reliable means for the quantity determination of lysozyme.
Li
Juan
J
Department of Chemistry, Nankai University, Tianjin, China.
He
Xi-Wen
XW
Wu
Yun-Li
YL
Li
Wen-You
WY
Zhang
Yu-Kui
YK
D002021:Buffers; D019187:Cadmium Compounds / Q000737:chemistry*; D003102:Colloids; D006863:Hydrogen-Ion Concentration; D009113:Muramidase / Q000032:analysis* / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D009994:Osmolar Concentration; D013691:Tellurium / Q000737:chemistry*; D013997:Time Factors
D002021:Buffers; D019187:Cadmium Compounds; D003102:Colloids; D009113:Muramidase; D013691:Tellurium; C028337:cadmium telluride