Detection of resistance to rifampicin and decreased susceptibility to penicillin in Neisseria meningitidis by real-time multiplex polymerase chain reaction assay.
In this study, we designed primers and probes for the rpoB gene of Neisseria meningitidis to detect rifampicin-resistant strains in a combined use with primers and probes previously described for penicillin intermediate isolates. The assay was set up in the Light Cycler instrument using the Fluorescence Resonance Energy Transfer platform. The method was applied to N. meningitidis strains and to culture-negative cerebrospinal fluids (CSFs) from patients with meningococcal invasive disease. A spiked CSF was used to determine the sensitivity of the assay.
Stefanelli
Paola
P
Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanita', 00161 Rome, Italy.
Neri
Arianna
A
Carattoli
Alessandra
A
Mastrantonio
Paola
P
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002555:Cerebrospinal Fluid / Q000382:microbiology; D017931:DNA Primers; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D006801:Humans; D009345:Neisseria meningitidis / Q000187:drug effects / Q000235:genetics*; D010403:Penicillin Resistance / Q000235:genetics; D010406:Penicillins / Q000494:pharmacology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012293:Rifampin / Q000494:pharmacology*; D012680:Sensitivity and Specificity
D000900:Anti-Bacterial Agents; D017931:DNA Primers; D010406:Penicillins; D012293:Rifampin