title: Identification of the genes GPD1 and GPD2 of Pichia jadinii.
abstract: Two genes coding for proteins with a high degree of sequence similarity to glycerol-3-phosphate dehydrogenases have been isolated from the yeast Pichiajadinii. Fragments of the genes were PCR-amplified with degenerated primers from genomic DNA of P. jadinii. Clones containing the full-length genes PjGPDI and PjGPD2 were isolated by screening genomic libraries. DNA sequencing revealed open reading frames (ORFs) of 1182 bp and 1185 bp for PjGpdlp and PjGpd2p, respectively. In a complementation study PjGPD1 rescued the growth defect of a Saccharomyces cerevisiae Agpdl mutant strain under osmotic stress, while complementation by PjGPD2 is temperature sensitive. The sequences of the PjGPD1 and PjGPD2 ORFs have been submitted to the EMBL Nucleotide Sequence Database under Accession No. AJ632339 and AJ632340, the sequences of the corresponding genomic DNA fragments under Accession No. AJ632341 and AJ635370, respectively.
authors: Institut für Genetik, Technische Universität Dresden, 01062, Dresden, Germany. kom@rcs.urz.tu-dresden.de
authors: Kai
authors: K
authors: Ostermann
authors: Marion
authors: M
authors: Richter
authors: Matthias
authors: M
authors: Zscharnack
authors: Romy
authors: R
authors: Rothe
authors: Thomas
authors: T
authors: Walther
authors: Gerhard
authors: G
authors: Rödel
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers; D005800:Genes, Fungal / Q000235:genetics*; D005816:Genetic Complementation Test; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics*; D008969:Molecular Sequence Data; D010843:Pichia / Q000201:enzymology / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D005993:Glycerolphosphate Dehydrogenase