Cell cycle of bone marrow CD34+ cells during autoimmune disease development in MRLMpJ/lpr mice.
The development of autoimmune disease in Fas-deficient MRLMpJ/lpr mice is associated with a relative decrease in the content of bone marrow CD34+ cells, which can attest to intensification of migration of early hemopoietic precursors from the bone marrow. The intensity of CD34+ apoptosis is high in young healthy MRLMpJ/lpr mice in comparison with the control, but decreases during the development of autoimmune disease. Proliferative activity of CD34+ cell population surpassed the control in all mouse groups, except AID2. The detected shifts in quantitative and qualitative parameters of CD34+ cells attest to an important role of stem hemopoietic precursors in the formation of autoimmune disease in MRLMpJ/lpr mice.
Feofanova
N A
NA
Institute of Clinical Immunology, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk. irorl@mail.ru
Toporkova
L B
LB
Tikhonova
M A
MA
Nevinskii
G A
GA
Kozlov
V A
VA
Orlovskaya
I A
IA
D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism*; D017209:Apoptosis / Q000502:physiology*; D001327:Autoimmune Diseases / Q000503:physiopathology*; D002453:Cell Cycle / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D003114:Colony-Forming Units Assay; D003124:Colorimetry; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D051379:Mice; D019463:Mice, Inbred MRL lpr; D011507:Proteinuria / Q000652:urine
D018952:Antigens, CD34