Enhanced cAMP/PKA pathway by seabuckthorn fatty acids in aged rats.
Seabuckthorn fatty acids were extracted by crushing and centrifuging from china seabuckthorn fruit. We detected cyclic nucleotides concentration in serum of different stages in aged rats (from 16 to 21 months), cyclic nucleotides concentration, PKA activity and PDE activity in hepatic tissue in aged rats by seabuckthorn fatty acids. Our data showed that the serum cAMP concentration decreased, accompany with the cGMP concentration increased and the imbalance of the cAMP/cGMP ratio in aged process. This kind of change equally in the hepatic tissue, the cAMP concentration decreased, PKA activity also decreased, but no change of the cAMP particularity PDE activity. And the SBFAs raised serum cAMP level in different stages, and raised the cAMP concentration and PKA activity of hepatic tissue, but did not effect the cAMP particularity PDE activity. Our study demonstrated that it is imbalance of the cAMP/cGMP ratio in aged process. SBFAs enhanced the cAMP/PKA pathway, regulated cAMP/cGMP ratio in aged rats.
Hu
Rui
R
Department of Pharmacology, Xi'an Jiaotong University, Xi'an, China.
Yuan
Bingxiang
B
Wei
Xiazhen
X
Zhao
Limei
L
Tang
Junjie
J
Chen
Dong
D
D000375:Aging; D000818:Animals; D000242:Cyclic AMP / Q000097:blood / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000097:blood / Q000378:metabolism*; D006152:Cyclic GMP / Q000097:blood / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005227:Fatty Acids / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D005638:Fruit / Q000737:chemistry; D031281:Hippophae / Q000737:chemistry*; D007118:Immunoassay; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D005227:Fatty Acids; D010936:Plant Extracts; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases; D006152:Cyclic GMP