Analysis of connective tissue growth factor promoter polymorphism in Thai children with biliary atresia.
OBJECTIVE
Connective tissue growth factor (CTGF) has been proposed to play a key role in the pathogenesis of hepatic fibrosis in biliary atresia (BA). The aim of the present study was to determine the single nucleotide polymorphism (SNP) in the promoter region of CTGF gene in a Thai population, and to investigate the possible role of CTGF promoter polymorphism in the susceptibility of BA.


METHODS
Genomic DNA was obtained from 84 patients with BA and 142 healthy controls. The -447 G/C and -132 C/G in CTGF promoter were amplified and examined by amplification-refractory mutation system (ARMs) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis, respectively. The test of Hardy-Weinberg equilibrium (HWE) was performed using HWE program of SNPAnalyzer. Statistical analysis was carried out with SPSS and Epi Info.


RESULTS
According to the previous experiment, there were two SNPs, which were at position -447 and -132 on the promoter. However, there was only one SNP at the position -447 in the Thai population. No significant differences in genotype and allele frequency were observed between BA and controls or with BA subgroups.


CONCLUSIONS
The present study demonstrated that CTGF polymorphism at -447 G/C was not associated with BA and the jaundice status of the postoperative BA patients.
Sa-nguanmoo
Pattaratida
P
Center of Excellence in Viral Hepatitis Research, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University.
Vejchapipat
Paisarn
P
Chongsrisawat
Voranush
V
Chirathaworn
Chintana
C
Honsawek
Sittisak
S
Theamboonlers
Apiradee
A
Poovorawan
Yong
Y
D000483:Alleles; D001656:Biliary Atresia / Q000235:genetics*; D016022:Case-Control Studies; D002648:Child; D003238:Connective Tissue; D055513:Connective Tissue Growth Factor; D004198:Disease Susceptibility; D005260:Female; D005838:Genotype; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics*; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics*; D008297:Male; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D013785:Thailand
C526317:CCN2 protein, human; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D055513:Connective Tissue Growth Factor