Fabrication of nanostructured hydroxyapatite and analysis of human osteoblastic cellular response.
Nano-sized hydroxyapatite (HA) powders were produced by a hydrothermal method and a precipitation method. Spark plasma sintering (SPS) was used to fabricate nanostructured HA (NHA) using nano-sized HA powders as a precursor. Conventional sintering was employed to produce microstructured HA (MHA). Characteristics of HA powders and HA bulk ceramics after sintering were investigated by XRD, FTIR, SEM, TEM, particle size distribution, and AFM. Dense compacts consisting of equiaxed grains with an average grain size of approximately 100 nm were obtained by SPS. Human osteoblasts were cultured on both NHA and MHA and cell attachment, proliferation, and mineralization were evaluated. After 90 min incubation, the cell density on NHA surface was significantly higher than that of MHA and glass control, whereas average cell area of a spread cell was significantly lower on NHA surface compared to MHA and glass control after 4 h incubation. Matrix mineralization was determined after 7 and 14 days incubation by using alizarin red assay combined with cetylpyridinium chloride extraction. NHA shows significant enhancement (p < 0.05) in mineralization compared to MHA. Results from this study suggest that NHA may be a much better candidate for clinical use in terms of bioactivity.
Guo
Xingyuan
X
Materials Science Centre, School of Materials, University of Manchester, Grosvenor Street, Manchester M1 7HS, United Kingdom.
Gough
Julie E
JE
Xiao
Ping
P
Liu
Jing
J
Shen
Zhijian
Z
D001672:Biocompatible Materials / Q000737:chemistry; D018786:Bone Substitutes; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D002516:Ceramics; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D017886:Durapatite / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D008903:Minerals / Q000378:metabolism; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D033362:Powder Diffraction; D011208:Powders; D019736:Prostheses and Implants; D013499:Surface Properties
D001672:Biocompatible Materials; D018786:Bone Substitutes; D008903:Minerals; D011208:Powders; D017886:Durapatite