title: [Evaluation of adiponectin and TNFalpha genes expression in women with gestational diabetes. Preliminary results].
abstract: OBJECTIVE
  Recent studies suggest the essential role of different cytokines realised from adipose tissue in pathogenesis of gestational diabetes. The aim of the study was evaluation of adiponectin (diabetes development protective factor) and TNFalpha (one of the most important insulin resistance mediator) genes expression in maternal visceral and subcutaneous adipose tissue as well as placental tissue.
  
  
  METHODS
  The study group consists of patients with gestational diabetes, healthy pregnant glucose tolerant women represented the control group. Tissue samples--placental tissue, visceral and subcutaneous adipose tissue--were obtained from patients who were undergoing ceasarean section. RT-PCR technique was performed for evaluation of adoponectin and TNF alpha genes expression.
  
  
  RESULTS
  Our study showed decreased adiponectin gene expression and increased TNFalpha gene expression in visceral adipose tissue in pregnant women with gestational diabetes. An expression of adiponectin gene in diabetic placental tissue was not observed. We also noticed slight increase of TNF alpha gene expressionin placenta of diabetic cases.
  
  
  CONCLUSIONS
  Decreased adiponectin and increased TNFalpha genes expression in adipose tissue of pregnant women with gestational diabetes seems to play a significant role in insulin resistance appearance and can lead to development of diabetes in pregnancy.
authors: Zakład Patofizjologii Ciazy AM w Białymstoku. mkuzmicki@op.pl
authors: Mariusz
authors: M
authors: Kuźmicki
authors: Jacek
authors: J
authors: Szamatowicz
authors: Adam
authors: A
authors: Kretowski
authors: Paweł
authors: P
authors: Kuć
authors: Marek
authors: M
authors: Kretowski
authors: Natalia
authors: N
authors: Wawrusiewicz
authors: Anna
authors: A
authors: Okruszko
authors: Dereck
authors: D
authors: Leroith
authors: Maria
authors: M
authors: Górska
mesh_terms: D052242:Adiponectin / Q000235:genetics* / Q000378:metabolism; D000328:Adult; D016640:Diabetes, Gestational / Q000235:genetics* / Q000378:metabolism; D005260:Female; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics*; D050152:Intra-Abdominal Fat / Q000737:chemistry; D008875:Middle Aged; D009765:Obesity / Q000235:genetics; D010920:Placenta / Q000737:chemistry; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018709:Statistics, Nonparametric; D050151:Subcutaneous Fat / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000378:metabolism
chemical_list: D052242:Adiponectin; D014409:Tumor Necrosis Factor-alpha