[A new polymer of 8,9-di-O-glucosylated 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid from a cell wall of Brevibacterium casei AEI Ac-2114T].
A polysaccharide containing the residues of 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid (Kdn) was found in the cell wall of the Brevibacterium casei strain AEI Ac-2114T . The polymer structure was elucidated by analyzing one-dimensional spectra of 1H and 13C NMR and bidimentional experiments 1H/13C-COSY, TOCSY, 1H/13C-gHSQC, and 1H/13C-gHMBC. The polymer is built up of the 2--> 4-linked Kdn residues substituted by beta-D-Glcp residues at 8- and 9-hydroxyls; such a polymer with disubstituted Kdn residues was found for the first time. A glycosylated teichoic acid of the 1,3-poly(glycerophosphate) type was also identified among other anionic polymers of cell wall.
Potekhina
N V
NV
Evtushenko
L I
LI
Senchenkova
S N
SN
Shashkov
A S
AS
D001951:Brevibacterium / Q000737:chemistry*; D002473:Cell Wall / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D011108:Polymers / Q000737:chemistry / Q000302:isolation & purification; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D013400:Sugar Acids / Q000032:analysis*; D013682:Teichoic Acids / Q000032:analysis
C519221:8,9-di-O-glucosylated 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid; D011108:Polymers; D011135:Polysaccharides, Bacterial; D013400:Sugar Acids; D013682:Teichoic Acids