A multi-channel electroporation microchip for gene transfection in mammalian cells.
We developed a multi-channel electroporation microchip made of polydimethylsiloxane (PDMS) and glass for gene transfer in mammalian cells. This chip produces multiple electric field gradients in a single microchip by varying the lengths of the microchannels from 2 to 4 cm. Electric fields of 0.65, 0.57, 0.49, 0.41, and 0.33 kV/cm were simultaneously produced in a single chip when the voltage of 1.3 kV was applied. We transferred enhanced green fluorescent protein genes (pEGFP) into HEK-293 and CHO cells, which were cultured within the microchannels. The feasibility of our device was demonstrated because it was able to produce five different transfection rates and survival rates at different electric fields produced in a single microchip. This system is expected to optimize the experimental conditions in gene transfection research more easily and faster than conventional electroporation methods.
Kim
Jeong Ah
JA
Digital Bio Technology Co., Institute of Advanced Machinery & Design, San 56-1, Seoul National University, Shinlim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
Cho
Keunchang
K
Shin
Young Shik
YS
Jung
Neoncheol
N
Chung
Chanil
C
Chang
Jun Keun
JK
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D018274:Electroporation / Q000295:instrumentation*; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D056656:Lab-On-A-Chip Devices; D010957:Plasmids; D014162:Transfection / Q000295:instrumentation*
D049452:Green Fluorescent Proteins