Morphine reciprocally regulates IL-10 and IL-12 production by monocyte-derived human dendritic cells and enhances T cell activation.
We evaluated the effect of morphine on human dendritic cells (DCs). Interestingly, immature DCs were found to express all 3 (mu, kappa, delta) opioid receptors on the cell surface. Chronic morphine treatment (10(-8) to 10(-12) M) during the development of DCs from monocytes augmented LPS-induced upregulation of HLA-DR, CD86, CD80, and CD83 and increased the T cell stimulatory capacity of DCs, which could be inhibited by naloxone, an opioid receptor antagonist. The change in surface phenotype was paralleled by a p38 MAPK-dependent decrease in IL-10 and increase in IL-12 secretion. Our data indicate that morphine exerts an immunostimulatory effect by modulating LPS-induced DC maturation.
Messmer
Davorka
D
Laboratory of Experimental Immunology, The Feinstein Institute for Medical Research, 350 Community Drive, Manhasset, NY, USA. dmessmer@ucsd.edu
Hatsukari
Ikusuke
I
Hitosugi
Naoko
N
Schmidt-Wolf
Ingo G H
IG
Singhal
Pravin C
PC
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology*; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D007165:Immunosuppression Therapy; D016753:Interleukin-10 / Q000096:biosynthesis*; D018664:Interleukin-12 / Q000096:biosynthesis*; D008213:Lymphocyte Activation / Q000187:drug effects*; D007959:Lymphocyte Culture Test, Mixed; D009000:Monocytes / Q000276:immunology*; D009020:Morphine / Q000494:pharmacology*; D009293:Opioid-Related Disorders / Q000276:immunology; D011725:Pyridines / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007093:Imidazoles; D011725:Pyridines; D016753:Interleukin-10; D018664:Interleukin-12; D009020:Morphine; D048051:p38 Mitogen-Activated Protein Kinases; C093642:SB 203580