title: Rapid detection of Escherichia coli O157:H7 spiked into food matrices.
abstract: Food poisoning causes untold discomfort to many people each year. One of the primary culprits in food poisoning is Escherichia coli O157:H7. While most cases cause intestinal discomfort, up to 7% of the incidences lead to a severe complication called hemolytic uremic syndrome which may be fatal. The traditional method for detection of E. coli O157:H7 in cases of food poisoning is to culture the food matrices and/or human stool. Additional performance-based antibody methods are also being used. The NRL array biosensor was developed to detect multiple antigens in multiple samples with little sample pretreatment in under 30 min. An assay for the specific detection of E. coli O157:H7 was developed, optimized and tested with a variety of spiked food matrices in this study. With no sample pre-enrichment, 5 x 10(3) cells mL(-1) were detected in buffer in less than 30 min. Slight losses of sensitivity (1-5 x 10(-4) cell mL(-1)) but not specificity occur in the presence of high levels of extraneous bacteria and in various food matrices (ground beef, turkey sausage, carcass wash, and apple juice). No significant difference was observed in the detection of E. coli O157:H7 in typical culture media (Luria Broth and Tryptic Soy Broth).
authors: Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, 4555 Overlook Avenue, SW, Washington, DC 20375, USA. lcs@cbmse.nrl.navy.mil <lcs@cbmse.nrl.navy.mil>
authors: Lisa C
authors: LC
authors: Shriver-Lake
authors: Stephanie
authors: S
authors: Turner
authors: Chris R
authors: CR
authors: Taitt
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D015374:Biosensing Techniques; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D005506:Food Contamination; D005516:Food Microbiology; D005517:Foodborne Diseases; D007118:Immunoassay / Q000379:methods; D008089:Listeria monocytogenes / Q000302:isolation & purification; D008460:Meat / Q000382:microbiology; D012486:Salmonella typhimurium / Q000302:isolation & purification
chemical_list: D000907:Antibodies, Bacterial