Chemiluminescence detection of organic peroxides in a two-phase system.
Potential application of chemiluminescence (CL) reaction of luminol for detection of organic peroxides by HPLC post-column reaction using an immiscible apolar eluent (hexane) with aqueous solution of luminol has been proposed. The positive influence of the addition of an anionic surface-active agent--sodium dodecyl sulphate (SDS) to the luminol solution on the CL intensity has been observed. The sensitivity of the method is greater for peroxyacid and hydroperoxide tested, and lesser for diacyl peroxide as compared to system with polar eluent miscible with solution of luminol. Our interpretation of observed results based on the extraction efficiency, CL kinetics and microemulsion formation has been suggested.
Baj
Stefan
S
Department of Chemical Organic Technology and Petrochemistry, The Silesian University of Technology, ul. Krzywoustego 4, 44-100 Gliwice, Poland. Stefan.Baj@polsl.pl <Stefan.Baj@polsl.pl>
Krawczyk
Tomasz
T
D000143:Acids / Q000737:chemistry; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002625:Chemistry, Organic / Q000379:methods*; D004655:Emulsions; D006861:Hydrogen Peroxide / Q000737:chemistry; D007700:Kinetics; D008165:Luminol / Q000737:chemistry; D000432:Methanol / Q000737:chemistry; D008956:Models, Chemical; D010545:Peroxides / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry / Q000494:pharmacology*; D014867:Water / Q000737:chemistry
D000143:Acids; D004655:Emulsions; D010545:Peroxides; D013501:Surface-Active Agents; D014867:Water; D012967:Sodium Dodecyl Sulfate; D008165:Luminol; D006861:Hydrogen Peroxide; D000432:Methanol