title: Targeting neural circuitry in zebrafish using GAL4 enhancer trapping.
abstract: We present a pilot enhancer trap screen using GAL4 to drive expression of upstream activator sequence (UAS)-linked transgenes in expression patterns dictated by endogenous enhancers in zebrafish. The patterns presented include expression in small subsets of neurons throughout the larval brain, which in some cases persist into adult. Through targeted photoconversion of UAS-driven Kaede and variegated expression of UAS-driven GFP in single cells, we begin to characterize the cellular components of labeled circuits.
authors: Department of Physiology, University of California, San Francisco, California 94158, USA.
authors: Ethan K
authors: EK
authors: Scott
authors: Lindsay
authors: L
authors: Mason
authors: Aristides B
authors: AB
authors: Arrenberg
authors: Limor
authors: L
authors: Ziv
authors: Nathan J
authors: NJ
authors: Gosse
authors: Tong
authors: T
authors: Xiao
authors: Neil C
authors: NC
authors: Chi
authors: Kazuhide
authors: K
authors: Asakawa
authors: Koichi
authors: K
authors: Kawakami
authors: Herwig
authors: H
authors: Baier
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001921:Brain / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D004268:DNA-Binding Proteins; D004742:Enhancer Elements, Genetic; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D009474:Neurons / Q000378:metabolism; D010865:Pilot Projects; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000235:genetics*; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*; D019076:Transgenes; D015027:Zebrafish / Q000196:embryology / Q000235:genetics* / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; C052130:GAL4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors