Screening for biomarkers in plasma from patients with gangrenous and phlegmonous appendicitis using CE and CEC in combination with MS.
Today a high degree of "false" appendicitis diagnoses are occurring. In this study, a screening experiment of biomarkers of two different kinds of appendicitis, gangrenous and phlegmonous, were conducted with CE and CEC coupled to MS. Plasma samples were obtained from patients pre- and post-surgery. A large amount of data was generated to be able to compare them, and chemometrics tools were utilized to visualize the differences. Indicative patterns were found for both pre- and post-surgery of the two types of inflammation as well as between them. The divergences were traced back to the MS peaks obtained in the CE- and CEC-MS setups as possible biomarkers for the two forms of appendicitis.
Johannesson
Nina
N
Analytical Chemistry, Department of Physical and Analytical Chemistry, Biomedical Centre, Uppsala University, Uppsala, Sweden.
Olsson
Louise
L
Bäckström
Daniel
D
Wetterhall
Magnus
M
Danielsson
Rolf
R
Bergquist
Jonas
J
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001062:Appendectomy; D001064:Appendicitis / Q000097:blood* / Q000145:classification* / Q000150:complications / Q000601:surgery; D015415:Biomarkers / Q000097:blood; D053801:Capillary Electrochromatography / Q000295:instrumentation / Q000379:methods*; D002481:Cellulitis / Q000097:blood* / Q000150:complications; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D005260:Female; D005734:Gangrene / Q000097:blood* / Q000150:complications; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D008875:Middle Aged; D010949:Plasma / Q000737:chemistry
D015415:Biomarkers