[Apoptotic study on the effect of fluorine and selenium on the human hair follicle in vitro].
OBJECTIVE
The aim of this study was to observe the human hair follicle apoptosis status affected by fluorine and the antagonism effect by selenium in vitro.


METHODS
The single hair follicles were separated and cultured, then they were added in different concentrations of sodium fluoride and sodium selenite. Chosen the appropriate concentrations, they were divided into 7 groups. The TUNEL was used to investigate the apoptotic cells of different parts. The morphous of hair follicles was observed consecutively and electron microscope was used.


RESULTS
We found that in 1 mmol/L and 10 mmol/L sodium fluoride groups, when the human hair follicles in vitro were cultured on the 5th day, the apoptotic cells of outer root sheath (ORS), dermal sheath and hair papilla, hair bulb were obviously increased. But 0.01 mmol/L sodium selenite weakened the toxicity of 1 mmol/L sodium fluoride at the outer root sheath and hair bulb (P < 0.05).


CONCLUSIONS
Different concentrations of sodium fluoride had different effect on the growth of human hair follicle in vitro which were cultured on 5th day. Sodium fluoride of certain concentration could accelerate the apoptosis of human hair follicle in vitro. Sodium selenite of certain concentration could act antagonism to the toxicity of sodium fluoride.
Tu
Jun-bo
JB
Department of Oral and Maxillary Surgery, Stornatological College, Xi'an Jiao Tong University, Xi'an 710004, China.
Yang
Zhuang-qun
ZQ
Xing
Zhe
Z
Xue
Ying
Y
Liu
Xiao-hua
XH
D000293:Adolescent; D000328:Adult; D017209:Apoptosis / Q000187:drug effects*; D018859:Hair Follicle / Q000187:drug effects*; D006801:Humans; D008875:Middle Aged; D012969:Sodium Fluoride / Q000494:pharmacology*; D018038:Sodium Selenite / Q000494:pharmacology; D046509:Tissue Culture Techniques; D055815:Young Adult
D012969:Sodium Fluoride; D018038:Sodium Selenite