Comparative study of the hyperbaric hyperoxygenation in ischemic colonic loops in rats.
OBJECTIVE
To analyze and to evaluate the effect of the hyperbaric hyperoxygenation in the mechanical resistance of ischemic colon of rats.


METHODS
Eighty rats, distributed in four groups of 20 animals in each one, were used. In group 1 (G1), the control group, ischemia was not caused. Group 2 was submitted to the lesser degree of ischemia. Group 3 was submitted to the intermediate degree of ischemia. In group 4, a bigger degree of intestinal ischemia was provoked. Each group was divided in two sub-groups of ten animals each: with hyperbaric chamber (CC) and without hyperbaric chamber (SC). The animals of the four CC subgroups were placed in an experimental hyperbaric chamber in order to inhale oxygen at 100%, at two Absolute Atmospheres, for 120 minutes, for a four-day consecutive period. The animals of the four SC subgroups were kept in environment air during the five days of the experiment. All animals have been submitted to the mechanical study of the intestinal loop by the pressure test of the rupture by liquid distension. The euthanasia occurred in the fifth post-operative day.


RESULTS
Considering the ischemia factor, the four groups were different among them (p=0.0001). There was no statistical difference between subgroups CC and SC (p=0.3461).


CONCLUSIONS
The hyperbaric oxygen-therapy did not present improvement on the induced ischemia in rats upright colic loop.
Rocha
Antonio Angelo
AA
Department of Surgery, Coloproctology Division, Faculty of Medical Sciences, Universidade Federal de Campinas, Brazil.
Coy
Cláudio Sadi Rodrigues
CS
Góes
Juvenal Ricardo Navarro
JR
Ayrizono
Maria de Lourdes Setsuko
Mde L
Wu
Feng Chung
FC
Fagundes
João José
JJ
D000714:Anastomosis, Surgical; D000818:Animals; D003106:Colon / Q000098:blood supply* / Q000601:surgery; D004195:Disease Models, Animal; D006931:Hyperbaric Oxygenation / Q000379:methods*; D007511:Ischemia / Q000628:therapy*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013314:Stress, Mechanical; D013718:Tensile Strength / Q000502:physiology; D014945:Wound Healing / Q000502:physiology*