title: A proline to glycine mutation in the Lck SH3-domain affects conformational sampling and increases ligand binding affinity.
abstract: Loop flexibility is discussed as a factor that affects ligand binding affinity of SH3 domains. To test this hypothesis, we designed a mutant in which a proline in the RT-loop of the human Lck SH3-domain is replaced by glycine. The dynamics and ligand binding properties of wild-type and mutant LckSH3 were studied by fluorescence and NMR spectroscopy as well as molecular dynamics simulations. Although the mutated residue does not form direct contacts with the ligand, the mutation increases ligand affinity by a factor of eight. The mutant exhibits increased loop flexibility and enhanced sampling of binding-competent conformations. This effect is expected to facilitate ligand binding itself and might also allow formation of tighter contacts in the complex thus resulting in an increased binding affinity.
authors: Abteilung Bioinformatik, Institut für Biochemie, Emil-Fischer-Zentrum, Universität Erlangen-Nürnberg, Fahrstr. 17, 91054 Erlangen, Germany.
authors: Finn
authors: F
authors: Bauer
authors: Heinrich
authors: H
authors: Sticht
mesh_terms: D000818:Animals; D005998:Glycine / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D008024:Ligands; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck) / Q000737:chemistry* / Q000235:genetics; D009154:Mutation; D011392:Proline / Q000737:chemistry* / Q000235:genetics; D011487:Protein Conformation; D018909:src Homology Domains / Q000235:genetics
chemical_list: D008024:Ligands; D011392:Proline; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D005998:Glycine