Dp71ab/DAPs complex composition changes during the differentiation process in PC12 cells.
PC12 cells express different Dp71 isoforms originated from alternative splicing; one of them, Dp71ab lacks exons 71 and 78. To gain insight into the function of Dp71 isoforms we identified dystrophin associated proteins (DAPs) that associate in vivo with Dp71ab during nerve growth factor (NGF) induced differentiation of PC12 cells. DAPs expression was analyzed by RT-PCR, Western blot and indirect immunofluorescence, showing the presence of each mRNA and protein corresponding to alpha-, beta-, gamma-, delta-, and epsilon-sarcoglycans as well as zeta-sarcoglycan mRNA. Western blot analysis also revealed the expression of beta-dystroglycan, alpha1-syntrophin, alpha1-, and beta-dystrobrevins. We have established that Dp71ab forms a complex with beta-dystroglycan, alpha1-syntrophin, beta-dystrobrevin, and alpha-, beta- and gamma-sarcoglycans in undifferentiated PC12 cells. In differentiated PC12 cells, the complex composition changes since Dp71ab associates only with beta-dystroglycan, alpha1-syntrophin, beta-dystrobrevin, and delta-sarcoglycan. Interestingly, neuronal nitric oxide synthase associates with the Dp71ab/DAPs complex during NGF treatment, raising the possibility that Dp71ab may be involved in signal transduction events during neuronal differentiation.
Romo-Yáñez
J
J
Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN, Avenida Instituto Politécnico Nacional 2508, Apartado Postal 14-740, C.P. 07000, Ciudad de México, México.
Ceja
V
V
Ilarraza-Lomelí
R
R
Coral-Vázquez
R
R
Velázquez
F
F
Mornet
D
D
Rendón
A
A
Montañez
C
C
D000818:Animals; D002135:Calcium-Binding Proteins / Q000032:analysis / Q000378:metabolism; D002454:Cell Differentiation; D049030:Dystroglycans / Q000378:metabolism; D016189:Dystrophin / Q000032:analysis / Q000378:metabolism*; D049029:Dystrophin-Associated Proteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000032:analysis / Q000378:metabolism; D009124:Muscle Proteins / Q000032:analysis / Q000378:metabolism; D020932:Nerve Growth Factor / Q000494:pharmacology; D009474:Neurons / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I / Q000032:analysis / Q000378:metabolism; D016716:PC12 Cells; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D049031:Sarcoglycans / Q000032:analysis / Q000235:genetics / Q000378:metabolism
D002135:Calcium-Binding Proteins; D016189:Dystrophin; D049029:Dystrophin-Associated Proteins; D008565:Membrane Proteins; D009124:Muscle Proteins; D012333:RNA, Messenger; D049031:Sarcoglycans; C085339:apo-dystrophin 1; C098885:dystrobrevin; C091825:syntrophin alpha1; D049030:Dystroglycans; D020932:Nerve Growth Factor; D052248:Nitric Oxide Synthase Type I