title: Safety and efficacy of an inactivated vaccine against Lactococcus garvieae in rainbow trout (Oncorhynchus mykiss).
abstract: We studied the safety and efficacy of an inactivated vaccine (Ichtiovac-Lg) against Lactococcus garvieae in rainbow trout (Oncorhynchus mykiss). In an initial dose-response experiment to test safety, we injected 50 rainbow trout weighing 30-40 g with a double dose of vaccine (0.2 ml) intraperitoneally. We observed these fish three times a day until day 50 post-vaccination when they were killed to evaluate visceral reactions, adhesions and intraperitoneal absorption. Survival was 100% in both the treatment and control groups and no significant differences were found in percentage of severe adhesions and pigmentation of peritonea and viscera. In a second trial, we injected 50 rainbow trout weighing 30-40 g with 0.1 ml of vaccine and a control group was injected with 0.1 ml of PBS intraperitoneally. On day 29 post-vaccination, both groups were challenged by intraperitoneal injection with 0.1 ml of a virulent heterologous strain of L. garvieae at 3 x 10(6) cfu ml(-1) and fish were observed for a further 21 days. At the end of the experiment, the survivals of the vaccinated fish and control group were 94 and 4%, respectively.
authors: Laboratory of Fish Pathology, Department of Animal Pathology, University of Zaragoza, c/Miguel Servet 177, 50013 Zaragoza, Spain.
authors: Daniel
authors: D
authors: Vendrell
authors: José Luis
authors: JL
authors: Balcázar
authors: Imanol
authors: I
authors: Ruiz-Zarzuela
authors: Ignacio
authors: I
authors: de Blas
authors: Olivia
authors: O
authors: Gironés
authors: José Luis
authors: JL
authors: Múzquiz
mesh_terms: D000818:Animals; D017756:Aquaculture; D001428:Bacterial Vaccines / Q000009:adverse effects / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D005393:Fish Diseases / Q000517:prevention & control*; D007274:Injections, Intraperitoneal / Q000662:veterinary; D017045:Lactococcus / Q000276:immunology*; D017686:Oncorhynchus mykiss / Q000276:immunology; D012449:Safety; D013290:Streptococcal Infections / Q000517:prevention & control / Q000662:veterinary*; D016896:Treatment Outcome; D014613:Vaccines, Attenuated / Q000276:immunology
chemical_list: D001428:Bacterial Vaccines; D014613:Vaccines, Attenuated