[Chromosomal alterations analyzed by comparative genomic hybridization in primary gastric carcinoma].
OBJECTIVE
To identify genetic abnormalities in primary gastric carcinoma.


METHODS
Comparative genomic hybridization (CGH) was used in screening DNA copy number changes along all chromosomes in 23 cases of primary gastric cancer.


RESULTS
Twenty-one out of 23 cases showed chromosomal losses and gains for at least one of the chromosomal arms in primary gastric cancer. The mean number of chromosomal alterations was 7.52. Chromosomal gains predominated over chromosomal losses in a ratio of 5.38:2.14. The most often involved chromosomal gains were observed in 8q (9/21, 42.9%), 20q (9/21, 42.9%), 17q (8/21, 38.1%), 3q (7/21, 33.3%), 7q (7/21, 33.3%), 11q (6/21, 28.6%), 13q (6/21, 28.6%), 1q (5/21, 23.8%) and 20p (5/21, 23.8%). The chromosomal arms with frequent losses were 17p (7/21, 33.3%), 18q (6/21, 28.6%), 5q (5/21, 23.8%), 8p (5/21, 23.8%), and 9p (5/21, 23.8%).


CONCLUSIONS
The phenomenon of gain and loss of chromosomal regions is observed in primary gastric cancer, which may induce the amplification of oncogenes and the loss of tumor suppressor genes to regulate the development and progression of gastric cancer.
Zhu
Ya-qing
YQ
Department of Surgery, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Medical College of Shanghai Jiaotong University, Shanghai 200025, China. liubyrj@yahoo.com.cn
Zhu
Zheng-gang
ZG
Liu
Bing-ya
BY
Chen
Xue-hua
XH
Yin
Hao-ran
HR
Wang
Xue-hao
XH
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002869:Chromosome Aberrations; D002872:Chromosome Deletion; D055028:Comparative Genomic Hybridization; D004247:DNA; D005260:Female; D020869:Gene Expression Profiling; D016147:Genes, Tumor Suppressor; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
D004247:DNA