title: The role of microsphere fabrication methods on the stability and release kinetics of ovalbumin encapsulated in polyanhydride microspheres.
abstract: The effect of microsphere fabrication methods on the stability and release kinetics of ovalbumin encapsulated in polyanhydride microspheres was investigated. The polyanhydrides used were poly(sebacic anhydride) (poly(SA)) and a 20:80 random copolymer of poly[1,6-bis(p-carboxyphenoxy)hexane] (poly(CPH)) and poly(SA). Microspheres were fabricated using three double emulsion methods (water/oil/water, water/oil/oil and solid/oil/oil) and cryogenic atomization. The encapsulation efficiency was highest for cryogenic atomization and lowest when the w/o/w technique was used. Microspheres fabricated by the s/o/o method had the largest initial burst of released protein. All the methods resulted in zero-order release of the protein after the burst. The release of ovalbumin from poly(SA) and 20:80 (CPH:SA) microspheres lasted approximately 3 and approximately 6 weeks, respectively. For all fabrication methods the primary structure of released ovalbumin was conserved as determined by gel electrophoresis. The secondary structure of ovalbumin encapsulated in 20:80 (CPH:SA) w/o/w microspheres was not conserved.
authors: Department of Chemical and Biological Engineering, Iowa State University, Ames, IA, USA.
authors: Amy S
authors: AS
authors: Determan
authors: Jennifer R
authors: JR
authors: Graham
authors: Katherine A
authors: KA
authors: Pfeiffer
authors: Balaji
authors: B
authors: Narasimhan
mesh_terms: D000818:Animals; D002214:Capsules; D002645:Chickens; D004337:Drug Carriers / Q000138:chemical synthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D010047:Ovalbumin / Q000737:chemistry*; D049388:Polyanhydrides / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D017433:Protein Structure, Secondary; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D002214:Capsules; D004337:Drug Carriers; D049388:Polyanhydrides; D011108:Polymers; D010047:Ovalbumin