A new antibacterial compound produced by an indigenous marine bacteria--fermentation, isolation, and biological activity.
The use of microorganisms for biological purpose has become an effective alternative to control pathogens. A marine bacterium Pseudomonas aeruginosa was isolated from Eal fish of Baluchistan coast of Pakistan. This strain produced a bactericidal antibiotic against environmental and clinical isolates. In this study, we purified bactericidal antibiotic from the ethyl acetate extract of the cells of P. aeruginosa and analyzed its chemical structure. Based on spectrometric analysis, this compound 1 is proposed to be 1-methyl-1,4 dihydroquinoline and is active against methicillin-resistant Staphylococcus aureus (MRSA), methicillin-sensitive S. aureus (MSSA), Salmonella typhi, Shigella flexneri, Escherichia coli, Proteus mirabilis, Vibrio aliginolyticus, Micrococcus luteus, Enterococcus faecalis, Enterobacter faecium but it is not active against G streptococci, Candida albicans, Aspergillus niger. Minimal inhibitory concentration for Gram-positive bacteria was between 50 and 75 microg mL(-1) and for Gram-negative bacteria 75-100 microg mL(-1).
Uzair
Bushra
B
Centre for Molecular Genetics, University of Karachi, Karachi 75270, Pakistan. shanibushra@yahoo.com
Ahmed
Nuzhat
N
Ahmad
Viqar Uddin
VU
Kousar
Farzana
F
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D005285:Fermentation; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D009904:Optical Rotation; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000378:metabolism; D011804:Quinolines / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D013055:Spectrophotometry, Infrared
D000900:Anti-Bacterial Agents; D011804:Quinolines