title: [Case report of a rare platelet-specific antigen HPA-10bw allele found in Chinese mainland].
abstract: A total of 1,000 Chinese blood donors were typed for human platelet antigens (HPA) using a sequence specific primers -polymerase chain reaction (PCR-SSP) based HPA genotyping method. An individual with a rare HPA-10w(a+b+) genotype was found. In order to confirm the typing results, a fragment of HPA-10 gene was amplified by PCR and then sequenced. Sequencing data showed that a single G to A substitution at nucleotide 263 occurred, resulting in amino acid change from Arg(CGA) to Gln(CAA) at position 62 of GPa protein. The substitution generated antigenic specificity HPA-10bw. The detection of an HPA-10bw allele in the Chinese population suggests that this rare allele should be considered in platelet alloimmunization, such as neonatal alloimmune thrombocytopenia (NAIT), post-transfusion thrombocytopenic purpura (PTP) and post-transfusion refractoriness to platelets (PTR).
authors: Shanghai Blood Center, Shanghai, China. fengml@sh163.net
authors: Ming-Liang
authors: ML
authors: Feng
authors: Tong
authors: T
authors: Shen
authors: Hui
authors: H
authors: Huang
authors: Wei
authors: W
authors: Shen
authors: Jian-Lian
authors: JL
authors: Wang
authors: Da-Zhuang
authors: DZ
authors: Liu
authors: Tong-Mao
authors: TM
authors: Zhao
mesh_terms: D000483:Alleles; D016824:Antigens, Human Platelet / Q000235:genetics*; D001483:Base Sequence; D002681:China; D005838:Genotype; D006801:Humans; D039661:Integrin beta3 / Q000235:genetics; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
chemical_list: D016824:Antigens, Human Platelet; D039661:Integrin beta3