Molar mass of main-chain bile acid-based oligo-esters measured by SEC, MALDI-TOF spectrometry and NMR spectroscopy: a comparative study.
Bile acid-based polymers are promising new materials for biomedical applications. The determination of their molar mass, as for other novel polymers, has been difficult, due to the lack of suitable standards for size exclusion chromatography (SEC). In order to solve this problem, a family of main-chain bile acid-based oligo-esters has been synthesized by acyclic diene metathesis to be used as analogues in such analysis. These oligomers have been characterized by SEC, MALDI-TOF mass spectrometry and NMR spectroscopy. The results show that SEC with polystyrene standards tends to overestimate the molar mass of these materials and that a correction factor between 0.50 and 0.60 should be used for more accuracy.
Gautrot
Julien E
JE
Département de chimie, Université de Montréal, C.P. 6128, succursale Centre-ville, Montréal, Québec H3C 3J7, Canada.
Zhu
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D001647:Bile Acids and Salts / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning; D002850:Chromatography, Gel / Q000379:methods*; D004952:Esters; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008970:Molecular Weight; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013055:Spectrophotometry, Infrared
D001647:Bile Acids and Salts; D004952:Esters