Development of a lateral-flow assay for rapid screening of the performance-enhancing sympathomimetic drug clenbuterol used in animal production; food safety assessments.
A lateral-flow assay that could provide visual evidence of the presence of clenbuterol in swine urine was developed. Colloidal gold was prepared and conjugated with anti-clenbuterol monoclonal antibody. Immunochromatographic test strips were produced, and then, 210 samples were tested on these strips. Analysis was completed in 10 min. Detection limit was 3 ppb of clenbuterol. Parallel GC-MS data indicated that clenbuterol rapid detection strip had no false negative. The false positive rate was 4.4%. Immunochromatographic strip has great applied value in the food safety field because it possesses benefits of sensitivity, stability, reproducibility, ease of use and inexpensive.
Lai
Weihua
W
The Key Laboratory of Food Science (Nanchang University), Ministry of Education, 330047, China. talktolaiwh@163.com
Xu
Yang
Y
Fung
Daniel Y C
DY
Xiong
Yonghua
Y
D000318:Adrenergic beta-Agonists / Q000032:analysis* / Q000652:urine; D000818:Animals; D000911:Antibodies, Monoclonal; D002976:Clenbuterol / Q000032:analysis* / Q000652:urine; D003257:Consumer Product Safety; D004350:Drug Residues / Q000032:analysis*; D005188:False Negative Reactions; D005189:False Positive Reactions; D005506:Food Contamination / Q000517:prevention & control*; D006801:Humans; D008403:Mass Screening; D008460:Meat / Q000032:analysis / Q000592:standards; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine / Q000652:urine*
D000318:Adrenergic beta-Agonists; D000911:Antibodies, Monoclonal; D011933:Reagent Kits, Diagnostic; D002976:Clenbuterol