Effect of ethanol extract of alga Laurencia supplementation on DNA oxidation and alkylation damage in mice.
Laurencia terpenoid extract (LET) had been extracted from the red alga Laurencia tristicha. The study is to investigate the effects of LET supplementation on DNA oxidation and alkylation damages in mice. Forty healthy Kunming mice weighing between 18g and 25g were randomly assigned into 4 groups, each consisting of ten animals. The mice were orally intubated respectively for 60 days with the designed concentrations of LET (25, 50,100mg/ kg b.w.) for three exposed groups and salad oil (0.2 ml) for the blank group. Food and water were free for the animals. Mice in the blank and exposed groups were sacrificed after the last treatment and the blood of each animal was quickly taken for further experiments. The spontaneous and oxidized DNA damages of peripheral lymphocytes induced by H2O2 were analysed by SCGE. O6-Methy-guanine (O6-MeG) was measured by high performance capillary zone electrophoresis. There was no significantly difference in DNA spontaneous damage on peripheral lymphocytes of all the mice. The oxidative DNA damage in the 50 mg/Kg body weight supplement group are 286AU with the oxidation of 10 micromol/L H2O2, significantly lower than the blank group 332AU (p<0.05). The contents of O6-MeG in plasma in the 50 mg/kg b.w. and 100mg/kg b.w. supplement group were 1.50 micromol/L and 1.88 micromol/L, significantly lower than that of the blank group, which was 2.89 micromol/L(p<0.05). The results from the present study indicated that the LET were rich in terpenoids and safety to be taken orally and it could improve antioxidative and decrease DNA damage effectively.
Liang
Hui
H
Institute of Human Nutrition, Medical College, Qingdao University, No. 38 Dengzhou Road, Qingdao, Shandong, China 266021. qdlianghui@126.com
He
Juan
J
Ma
Ai-guo
AG
Zhang
Pei-hua
PH
Bi
Shu-lian
SL
Shi
Da-yong
DY
D000818:Animals; D001681:Biological Assay; D020552:Comet Assay; D004247:DNA / Q000037:antagonists & inhibitors* / Q000633:toxicity; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors / Q000494:pharmacology; D044722:Laurencia / Q000737:chemistry*; D008214:Lymphocytes / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000494:pharmacology* / Q000633:toxicity; D011897:Random Allocation
D016166:Free Radical Scavengers; D010936:Plant Extracts; D004247:DNA; D006861:Hydrogen Peroxide