Immune response of BALB/c mouse immunized with recombinant MSPs proteins of Anaplasma marginale binding to immunostimulant complex (ISCOM).
Anaplasmosis, caused by Anaplasma marginale, results in significant economic losses of cattle in tropical and subtropical regions worldwide. Six major surface proteins (MSPs) were well characterized and designated as MSP1, MSP2, MSP3, MSP4, and MSP5. The objective of this study was to evaluate the humoral immune response of BALB/c mice against the recombinant MSPs, incorporated into immunostimulating complex (ISCOM). The recombinant proteins purified by Ni-NTA columns were incorporated into ISCOM and ISCOMATRIX by the lipid film hydration method. BALB/c mice immunized with ISCOM/rMSPs and ISCOMATRIX/rMSPs vaccines produced whole IgG, IgG1, and IgG2a, in contrast to the negative groups (PBS and ISCOMATRIX adjuvant). All groups that received antigen responded specifically against the rMSPs by Western blotting, showing the rMSP1a (60-105kDa), rMSP1b (100kDa), rMSP4 (47kDa), and rMSP5 (29kDa). Additional studies will have to be performed in cattle to evaluate the humoral and cellular mechanisms of this subunit vaccine and their possible use as protective vaccines against homologous and heterologous strains of A. marginale.
Kawasaki
Paula M
PM
Universidade Estadual de Londrina, Departamento. Medicina Veterinária Preventiva, CCA, Campus Universitário, Caixa Postal 6001, 86051-970 Londrina, PR, Brazil.
Kano
Flora S
FS
Tamekuni
Katia
K
Garcia
João Luis
JL
Marana
Elizabete R M
ER
Vidotto
Odilon
O
Vidotto
Marilda C
MC
D042322:Anaplasma marginale / Q000276:immunology*; D000712:Anaplasmosis / Q000276:immunology / Q000517:prevention & control*; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology; D001428:Bacterial Vaccines; D005260:Female; D016882:ISCOMs / Q000276:immunology*; D008565:Membrane Proteins / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000276:immunology*
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D016882:ISCOMs; D008565:Membrane Proteins; C085498:Msp4 protein, Anaplasma marginale; D011994:Recombinant Proteins; C449089:major surface protein 1a, Anaplasma; C450434:major surface protein 1b, Anaplasma; C111578:major surface protein 5, Anaplasma