HOMER2 binds MYO18B and enhances its activity to suppress anchorage independent growth.
MYO18B is a class XVIII myosin, cloned as a tumor suppressor gene candidate. To investigate the mechanisms of MYO18B-dependent tumor suppression, MYO18B-interacting proteins were searched for by a yeast two-hybrid screen. HOMER2, a Homer/Ves1 family protein, was identified as a binding partner of MYO18B. These proteins co-localized in the regions of membrane protrusion and stress fiber, which are known as ones with filamentous actin-rich structures. Expression of HOMER2 enhanced the ability of MYO18B to suppress anchorage-independent growth. These results indicate that HOMER2 and MYO18B cooperate together in tumor suppression.
Ajima
Rieko
R
Biology Division, National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo 104-0045, Japan.
Kajiya
Keiko
K
Inoue
Takeshi
T
Tani
Masachika
M
Shiraishi-Yamaguchi
Yoko
Y
Maeda
Masahiro
M
Segawa
Tatsuya
T
Furuichi
Teiichi
T
Sutoh
Kazuo
K
Yokota
Jun
J
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology*; D002462:Cell Membrane / Q000502:physiology*; D049109:Cell Proliferation; D000072233:Homer Scaffolding Proteins; D051379:Mice; D009218:Myosins / Q000378:metabolism*; D041681:NIH 3T3 Cells; D011485:Protein Binding; D025521:Tumor Suppressor Proteins / Q000378:metabolism*
D002352:Carrier Proteins; D000072233:Homer Scaffolding Proteins; C000606625:Homer2 protein, mouse; C500982:MYO18B protein, human; D025521:Tumor Suppressor Proteins; D009218:Myosins