Quartz crystal microbalance immunosensor for the detection of antibodies to double-stranded DNA.
We report the development of a novel quartz crystal microbalance immunosensor with the simultaneous measurement of resonance frequency and motional resistance for the detection of antibodies to double-stranded DNA (dsDNA). The immobilization of poly(L-lysine) and subsequent complexation with DNA resulted in formation of a sensitive dsDNA-containing nanofilm on the surface of a gold electrode. Atomic force microscopy has been applied for the characterization of a poly(L-lysine)-DNA film. After the blocking with bovine serum albumin, the immunosensor in flow-injection mode was used to detect the antibodies to dsDNA in purified protein solutions of antibodies to dsDNA and to single-stranded DNA, monoclonal human immunoglobulin G, DNase I and in blood serum of patients with bronchial asthma and systemic lupus erythematosus. Experimental results indicate high sensitivity and selectivity of the immunosensor.
Fakhrullin
Rawil F
RF
Department of Biochemistry, Kazan State University, Kremlevskaya 18, 420008, Kazan, Russia. biosensor@bk.ru
Vinter
Victor G
VG
Zamaleeva
Alsu I
AI
Matveeva
Maria V
MV
Kourbanov
Roustem A
RA
Temesgen
Belayhun K
BK
Ishmuchametova
Dilara G
DG
Abramova
Zinaida I
ZI
Konovalova
Olga A
OA
Salakhov
Myakzyum K
MK
D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D000974:Antibodies, Antinuclear / Q000097:blood*; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D001249:Asthma / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D004247:DNA / Q000737:chemistry / Q000276:immunology; D004277:DNA, Single-Stranded / Q000276:immunology; D003851:Deoxyribonucleases / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007074:Immunoglobulin G / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D018625:Microscopy, Atomic Force; D011107:Polylysine / Q000737:chemistry; D011791:Quartz / Q000737:chemistry*; D015203:Reproducibility of Results; D012710:Serum Albumin, Bovine / Q000737:chemistry
D000906:Antibodies; D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D004277:DNA, Single-Stranded; D007074:Immunoglobulin G; D011791:Quartz; D011107:Polylysine; D012710:Serum Albumin, Bovine; D006046:Gold; D004247:DNA; D003851:Deoxyribonucleases