High-throughput telomere length quantification by FISH and its application to human population studies.
A major limitation of studies of the relevance of telomere length to cancer and age-related diseases in human populations and to the development of telomere-based therapies has been the lack of suitable high-throughput (HT) assays to measure telomere length. We have developed an automated HT quantitative telomere FISH platform, HT quantitative FISH (Q-FISH), which allows the quantification of telomere length as well as percentage of short telomeres in large human sample sets. We show here that this technique provides the accuracy and sensitivity to uncover associations between telomere length and human disease.
Canela
Andrés
A
Telomeres and Telomerase Group, Molecular Oncology Program, Spanish National Cancer Centre, 3 Melchor Fernández Almagro, Madrid E-28029, Spain.
Vera
Elsa
E
Klatt
Peter
P
Blasco
María A
MA
D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D005260:Female; D005828:Genetics, Population; D006367:HeLa Cells; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D051379:Mice; D008875:Middle Aged; D009369:Neoplasms / Q000235:genetics*; D012737:Sex Factors; D016615:Telomere / Q000648:ultrastructure*