Natronorubrum sulfidifaciens sp. nov., an extremely haloalkaliphilic archaeon isolated from Aiding salt lake in Xin-Jiang, China.
An extremely haloalkaliphilic archaeon, strain AD2(T), was isolated from Aiding salt lake in Xin-Jiang, China. Strain AD2(T) required at least 12 % NaCl for growth. MgCl(2) was not required. The isolate was able to grow over a pH range of 8.0-10.0 and temperature range of 20-55 degrees C, with optimal growth at pH 8.7-9.2 and 44-47 degrees C. The major polar lipids of strain AD2(T) were phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester; glycolipids were not detected. Analysis of its 16S rRNA gene sequence indicated that strain AD2(T) was phylogenetically related to members of the genus Natronorubrum, with sequence similarities to the type strains of Natronorubrum bangense, Natronorubrum tibetense and Natronorubrum aibiense of 97.1, 95.9 and 96.1 %, respectively. The G+C content of its DNA was 60.9 mol% (T(m)). Levels of DNA-DNA relatedness between strain AD2(T) and the type strains of Nrr. bangense, Nrr. tibetense and Nrr. aibiense were 49, 38 and 41 %, respectively. It was concluded that strain AD2(T) represents a novel species of the genus Natronorubrum, for which the name Natronorubrum sulfidifaciens sp. nov. is proposed. The type strain is AD2(T) (=CGMCC 1.6307(T)=JCM 14089(T)).
Cui
Heng-Lin
HL
School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, People's Republic of China.|Graduate University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.|State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.
Tohty
Dilbr
D
College of Life Sciences, Xin-Jiang Normal University, Urumqi 830053, People's Republic of China.
Liu
Hong-Can
HC
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.
Liu
Shuang-Jiang
SJ
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.
Oren
Aharon
A
Institute of Life Sciences and the Moshe Shilo Minerva Center for Marine Biogeochemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Zhou
Pei-Jin
PJ
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.
D001482:Base Composition; D002681:China; D019641:DNA, Archaeal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005618:Fresh Water / Q000382:microbiology*; D006216:Halobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012965:Sodium Chloride
D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride