[Etiologic investigation of chronic granulomatous inflammation of hand by polymerase chain reaction and DNA sequencing].
OBJECTIVE
To explore the causative pathogens in littoral hand infections which exhibited chronic granulomatous inflammation, the relationship between chronic granulomatous inflammation and mycobacteria and to discuss the prospects of PCR in clinical application for diagnosis of granulomatous inflammation.


METHODS
With 16S-rDNA as the target sequence, Nest-PCR was used to detect mycobacteria directly from 37 cases of chronic granulomatous inflammations, and identified them by gene sequencing.


RESULTS
Twenty-four of 37 cases were positive for mycobacteria by Nest-PCR, in which 17 were M.marinum, 1 M.chelonae, 2 M.avium, 2 M.kansasii, and 2 M.tubercular through gene sequencing.


CONCLUSIONS
Nest-PCR combining gene sequencing proved to be a liable and sensitive method to detect Non-tubercular mycobacteria (NTM) in fresh tissue. NTM is the major factor of hand specific chronic infections other than tubercular. Pathological changes are difficult to differentiate TB from NTM and bacterial evidence was necessary.
Li
Xue-yuan
XY
Department of Hand Surgery, Sixth Hospital, Ningbo 315040, China.
Tan
Li
L
Xie
Hui
H
Zhang
Wei-wen
WW
Zhu
Yun-song
YS
Chen
De-song
DS
D002908:Chronic Disease; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006099:Granuloma / Q000175:diagnosis / Q000382:microbiology*; D006225:Hand; D006801:Humans; D007249:Inflammation / Q000175:diagnosis / Q000382:microbiology*; D025202:Molecular Diagnostic Techniques; D009165:Mycobacterium Infections, Nontuberculous / Q000175:diagnosis / Q000382:microbiology*; D019910:Mycobacterium marinum / Q000235:genetics / Q000302:isolation & purification; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification; D009170:Nontuberculous Mycobacteria / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S