[Study on the interaction between p-octacarboxyphenylazocalix[8]arene and norfloxacin by fluorescence spectrometry].
In the present paper, p-octacarboxyphenylazocalix[8]arene (CPAC) was used as supramolecular probe according to a reported method. The interaction of CPAC with drug norfloxacin (NFLX) was studied by fluorescence spectrometry. The results show that CPAC can strongly quench the fluorescence of NFLX because of the complex interaction between host and guest molecules in exo-inclusion complex. The spectral changes indicated that the quenching can be considered as static quenching mode. The hydrophobic interaction between the cavity of CPAC and the quinoline ring was the main force to consolidate the exo-inclusive complex CPAC-NFLX stability. The complex constant (K) and binding ratio (n) were determined to be 6. 38 X 10(5) L x mol(-1) and 1, respectively. Further experiment found that the calf thymus DNA and CPAC can combine, leading to the release of NFLX and the enhancement of fluorescence of the reaction system. It is expected that CPAC will be used as drug carrier and releaser.
Li
Yan-ping
YP
Center of Analysis and Testing, Nanchang University, Nanchang 330047, China.
Li
Lai-sheng
LS
Huang
Zhi-bing
ZB
Liu
Xu
X
Ge
Xiao-hui
XH
D000818:Animals; D047250:Calixarenes / Q000032:analysis* / Q000737:chemistry; D002417:Cattle; D004247:DNA / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D009643:Norfloxacin / Q000032:analysis* / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013696:Temperature; D047392:beta-Cyclodextrins / Q000737:chemistry
D047392:beta-Cyclodextrins; C549255:octacarboxyphenylazocalix(8)arene; D047250:Calixarenes; D004247:DNA; C427203:calf thymus DNA; C031215:betadex; D009643:Norfloxacin