title: Independent functions of Slit-Robo repulsion and Netrin-Frazzled attraction regulate axon crossing at the midline in Drosophila.
abstract: Slit and Netrin and their respective neuronal receptors play critical roles in patterning axonal connections in the developing nervous system by regulating the decision of whether or not to cross the midline. Studies of both invertebrate and vertebrate systems support the idea that Netrin, secreted by midline cells, signals through DCC (Deleted in Colorectal Carcinoma)/UNC40/Frazzled receptors to attract commissural axons toward and across the midline, whereas Slit signals through Robo family receptors to prevent commissural axons from recrossing the midline, as well as to prevent ipsilateral axons from ever crossing. Recent evidence from both Xenopus neuronal cell culture and Drosophila genetics have suggested that these signals may interact more directly in a hierarchical relationship, such that one response extinguishes the other. Here we present loss- and gain-of-function genetic evidence showing that the influence of Slit and Netrin on midline axon crossing is dictated by both independent and interdependent signaling functions of the Robo and Frazzled (Fra) receptors. Our results are not consistent with the proposal based on genetic analysis in Drosophila that the sole function of Slit and Robo during midline guidance is to repress Netrin attraction.
authors: Department of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA.
authors: David S
authors: DS
authors: Garbe
authors: Greg J
authors: GJ
authors: Bashaw
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology*; D019521:Body Patterning / Q000235:genetics; D004330:Drosophila / Q000196:embryology* / Q000235:genetics*; D029721:Drosophila Proteins / Q000378:metabolism*; D015870:Gene Expression; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D000075386:Netrin Receptors; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000378:metabolism*; D000097905:Roundabout Proteins
chemical_list: D029721:Drosophila Proteins; D009419:Nerve Tissue Proteins; D000075386:Netrin Receptors; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D000097905:Roundabout Proteins; C000617310:fra protein, Drosophila; C066819:sli protein, Drosophila