Immobilization of uricase within polystyrene using polymaleimidostyrene as a stabilizer and its application to uric acid sensor.
A novel amperometric uric acid (UA) sensor has been developed by coating the surface of a gold electrode with a polystyrene (PS) membrane formed by 30 microL of a 30 mg mL(-1) PS chloroform solution combined with 30 microL of a 5 mg mL(-1) polymaleimidostyrene (PMS) solution as a dispersant for enzyme, uricase; this membrane has been successfully employed as an immobilization support for uricase. In the PS membrane, PMS forms micelle-like structures containing uricase in an active state. This immobilized uricase membrane permits the permeation of oxygen, which is consumed by the uricase reaction. A good linear relationship is obtained over the concentration range of 5-105 microM. The concentration of uric acid was determined at a negative potential based on the decrease in the reduction current of oxygen and the interference of l-ascorbic acid can be completely eliminated.
Wang
Xiuyun
X
Department of Materials & Science, Graduate School of Engineering, Saitama Institute of Technology, 1690 Fusaiji Fukaya, Saitama 369-0293, Japan.
Hagiwara
Tokio
T
Uchiyama
Shunichi
S
D001205:Ascorbic Acid / Q000737:chemistry; D015374:Biosensing Techniques; D002725:Chloroform / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004563:Electrochemistry / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D008301:Maleimides / Q000737:chemistry*; D008823:Micelles; D008855:Microscopy, Electron, Scanning; D008956:Models, Chemical; D010100:Oxygen / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry*; D014503:Urate Oxidase / Q000032:analysis / Q000737:chemistry*; D014527:Uric Acid / Q000737:chemistry*; D015003:Yeasts / Q000201:enzymology
D004800:Enzymes, Immobilized; D008301:Maleimides; D008823:Micelles; D011137:Polystyrenes; C512149:polymaleimidostyrene; D014527:Uric Acid; D002725:Chloroform; D014503:Urate Oxidase; D001205:Ascorbic Acid; D010100:Oxygen