Phage therapy of Pseudomonas aeruginosa infection in a mouse burn wound model.
Mice compromised by a burn wound injury and subjected to a fatal infection with Pseudomonas aeruginosa were administered a single dose of a Pseudomonas aeruginosa phage cocktail consisting of three different P. aeruginosa phages by three different routes: the intramuscular (i.m.), subcutaneous (s.c.), or intraperitoneal (i.p.) route. The results of these studies indicated that a single dose of the P. aeruginosa phage cocktail could significantly decrease the mortality of thermally injured, P. aeruginosa-infected mice (from 6% survival without treatment to 22 to 87% survival with treatment) and that the route of administration was particularly important to the efficacy of the treatment, with the i.p. route providing the most significant (87%) protection. The pharmacokinetics of phage delivery to the blood, spleen, and liver suggested that the phages administered by the i.p. route were delivered at a higher dose, were delivered earlier, and were delivered for a more sustained period of time than the phages administered by the i.m. or s.c. route, which may explain the differences in the efficacies of these three different routes of administration.
McVay
Catherine S
CS
Department of Microbiology and Immunology, Texas Tech University Health Sciences Center, 3601 4th Street, Lubbock, TX 79430, USA.
Velásquez
Marisela
M
Fralick
Joe A
JA
D000818:Animals; D002056:Burns / Q000150:complications*; D004195:Disease Models, Animal; D005260:Female; D007273:Injections, Intramuscular; D007274:Injections, Intraperitoneal; D007279:Injections, Subcutaneous; D051379:Mice; D011552:Pseudomonas Infections / Q000382:microbiology / Q000401:mortality / Q000628:therapy*; D017105:Pseudomonas Phages / Q000502:physiology*; D011550:Pseudomonas aeruginosa / Q000821:virology*; D016896:Treatment Outcome; D014946:Wound Infection / Q000382:microbiology / Q000401:mortality / Q000628:therapy*