Analysis of Central European Corydalis species by nonaqueous capillary electrophoresis-electrospray ion trap mass spectrometry.
In the presented study, Central European Corydalis species, namely C. cava, C. intermedia, C. pumila, and C. solida were investigated by nonaqueous capillary electrophoresis-electrospray ion trap mass spectrometry (NACE-ESI-MS) utilizing an electrolyte consisting of 50 mM ammonium acetate, 1 M acetic acid and 10% methanol in acetonitrile, applying a separation voltage of 30 and 20 kV for 1 s for injection. Isopropanol-water (1:1) was used as sheath liquid at a flow-rate of 3 microl/min. Peak assignment was assisted by multistage-ESI-mass spectrometry (ESI-MSn). The optimized method was fully validated (RSD inter- and intraday < 5%, LOD < 2.3 microg/ml, LOQ < 27.6 microg/ml, recovery rates > 98.8%) and subsequently applied for the qualitative and quantitative determination of isoquinoline alkaloids in single plant tubers (sample size < 0.5 g) of the four Central European Corydalis species, each of them showing a characteristic and unique alkaloid pattern. Application of a principal component analysis (PCA) to the complete dataset of 39 analytes and 79 samples allowed the identification of 8 analytes responsible for lot discrimination. Hierarchical cluster analysis and descriptive statistical methods were used to confirm the findings of the explorative PCA.
Sturm
Sonja
S
Institute of Pharmacy/Pharmacognosy, Center of Molecular Biosciences, Leopold Franzens University Innsbruck, Innrain 52, A-6020 Innsbruck, Austria. Sonja.sturm@uibk.ac.at
Seger
Christoph
C
Stuppner
Hermann
H
D000470:Alkaloids / Q000737:chemistry; D001317:Austria; D001599:Berberine / Q000031:analogs & derivatives; D001600:Berberine Alkaloids; D031311:Corydalis / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D009862:Online Systems; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012995:Solubility; D012997:Solvents / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D000470:Alkaloids; D001600:Berberine Alkaloids; D010936:Plant Extracts; D012997:Solvents; C034384:coptisine; D001599:Berberine; C109505:allocryptopine