title: Borrelia burgdorferi potently activates bone marrow-derived conventional dendritic cells for production of IL-23 required for IL-17 release by T cells.
abstract: Lyme borreliosis is characterized by cellular inflammatory responses at multiple body sites. Recently, an association of interleukin-17 (IL-17) and Lyme arthritis was suggested. In this context, it is of special interest that the heterodimeric cytokine IL-23 can act on T cells and initiate the up-regulation of effector cytokines such as IL-17. To determine the role of this specific cytokine cascade for the induction of subsequently induced proinflammatory events we developed an in vitro system to investigate the IL-23-inducing capacity of Borrelia burgdorferi and the potential of the spirochete for inducing the IL-23/IL-17 axis. We used cells derived from mice deficient for IL-23 or IL-12 only or deficient for both IL-12 and IL-23 to define precisely the function of these cytokines. Experiments with bone marrow-derived dendritic cells (BMDC) identified these cells as sources for IL-23 but not for IL-12 after B. burgdorferi exposure. Subsequent investigations with T cell-depleted splenocyte fractions revealed a tight IL-23/IL-17 axis in response to the spirochetes. Monoclonal antibodies that block IL-23 showed further that BMDC-derived IL-23 was required for production of IL-17 in this experimental model. These in vitro data describing a spirochete-induced release of IL-23 may help to define IL-17-dependent inflammatory responses in the disease.
authors: Institute of Immunology, College of Veterinary Medicine, University of Leipzig, Leipzig, Germany.
authors: Jens
authors: J
authors: Knauer
authors: Sabine
authors: S
authors: Siegemund
authors: Uwe
authors: U
authors: Müller
authors: Samiya
authors: S
authors: Al-Robaiy
authors: Robert A
authors: RA
authors: Kastelein
authors: Gottfried
authors: G
authors: Alber
authors: Reinhard K
authors: RK
authors: Straubinger
mesh_terms: D000818:Animals; D025065:Borrelia burgdorferi / Q000276:immunology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology* / Q000382:microbiology*; D018664:Interleukin-12 / Q000172:deficiency; D020381:Interleukin-17 / Q000378:metabolism*; D053759:Interleukin-23 / Q000096:biosynthesis* / Q000172:deficiency; D008212:Lymphocyte Depletion; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D020381:Interleukin-17; D053759:Interleukin-23; D018664:Interleukin-12