[Polysaccharide and lipid composition of the brown seaweed Laminaria gurjanovae].
Polysaccharide and lipid composition of the Pacific brown seaweed Laminaria gurjanovae is determined. Alginic acid is shown to be the main polysaccharide of its biomass (about 28%); it consists of mannuronic and guluronic acid residues at a ratio of 3 : 1. The yield of water-soluble polymannuronic acid is low and does not exceed 1.1% of dry biomass. High laminaran content (about 22%) is found, whereas the yield of fucoidan is no more than 3.6%. Laminaran consists of two fractions, soluble and insoluble in cold water, their ratio is 2.5 : 1. Practically, insoluble laminaran is a linear 1,3-beta-D-glucan, and the soluble fraction was shown to be 1,3;1,6- 3-D-glucan. The oligosaccharide products of desulfation or partial acidic hydrolysis of fucoidan were studied by MALDI TOF MS; they were found to be fuco- and galactooligosaccharides. The fucoidan is suggested to be a highly sulfated partially acetylated galactofucan (Fuc/Gal is -1 : 1). The main lipid components of the dried L. gurjanovae are neutral lipids and glyceroglycolipids, whereas phospholipids are found in minor amounts. The main fatty acid components of lipids are 14 : 0, 16 : 0, 16 : 1 n-7, 18 : 1 n-7, and 18 : 2 n-6 acids.
Shevchenko
N M
NM
Anastiuk
S D
SD
Gerasimenko
N I
NI
Dmitrenok
P S
PS
Isakov
V V
VV
Zviagintseva
T N
TN
D000464:Alginates / Q000032:analysis; D005936:Glucans; D007795:Laminaria / Q000737:chemistry*; D008055:Lipids / Q000032:analysis*; D011134:Polysaccharides / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000464:Alginates; D005936:Glucans; D008055:Lipids; D011134:Polysaccharides; C008247:laminaran; C007789:fucoidan