A combined method for the determination of myocardial perfusion in experimental animals using microspheres and CMR.
A convenient method is introduced for myocardial perfusion research by combining multislice short-axis cine cardiovascular magnetic resonance (MSA-CMR) and colored microspheres (CM). In canine control-ischemia-reperfusion (n = 11), Cardiac output (CO) was measured using MSA-CMR (COMSA), Phase Contrast CMR (PC-CMR, COPC) and CM (from reference blood samples, COmicro) in 3 experimental periods per animal. COmicro significantly and systematically overestimated COMSA (median deviation: 291 mL/min, p < 0.01), while there was excellent agreement between PC-CMR and MSA-CMR without significant over-or underestimation (median deviation: -14 ml/min, p = NS). In quantitative myocardial perfusion assay by CM, CMR should be used instead of reference blood sampling. This should improve the accuracy of absolute regional perfusion measurement.
Surányi
Pál
P
The Department of Biochemistry, Molecular Genetics University of Alabama, Birmingham, Alabama 35294-0005, USA.
Kiss
Pál
P
Simor
Tamás
T
Elgavish
Ada
A
Elgavish
Gabriel A
GA
D000704:Analysis of Variance; D000818:Animals; D002302:Cardiac Output / Q000502:physiology*; D003287:Contrast Media / Q000008:administration & dosage*; D003326:Coronary Circulation / Q000502:physiology*; D004285:Dogs; D007089:Image Enhancement / Q000379:methods; D019028:Magnetic Resonance Imaging, Cine / Q000379:methods*; D008297:Male; D008863:Microspheres; D017202:Myocardial Ischemia / Q000503:physiopathology*; D018709:Statistics, Nonparametric
D003287:Contrast Media