title: Effect of parenteral and early intrajejunal nutrition on pancreatic digestive enzyme synthesis, storage and discharge in dog models of acute pancreatitis.
abstract: OBJECTIVE
  To study the effect of early intrajejunal nutrition on enzyme-protein synthesis and secretion during acute pancreatitis.
  
  
  METHODS
  Fifteen dogs were randomly divided into parenteral nutrition (n = 7) and early intrajejunal nutrition groups (n = 8). An acute pancreatitis model was induced by injecting 5% sodium taurocholate and trypsin into the pancreas via the pancreatic duct. Intrajejunal nutrition was delivered with a catheter via a jejunostomy tube after the model was established for 24 h. On d 1 and 7 and at the beginning of nutritional support, radioactive tracing and electron microscopes were used to evaluate the enzyme-protein synthesis in acinar cells, the subcellular fractionation and the change in zymogen granules after 1.85 x 10(6) Bq L-(3)H phenylalanine was infused at 30, 60, 120, and 180 min.
  
  
  RESULTS
  The 3H radioactivity in pancreatic acinar cells reached its peak level at 60 min, and the contents in the early intrajejunal nutrition group were higher than those in the parenteral nutrition group, which were then decreased. The mean number and area of zymogen granules did not show any significant statistical difference in both groups on d 1 or on d 7 (P > 0.05).
  
  
  CONCLUSIONS
  Early intrajejunal nutrition might be effective in dogs with acute pancreatitis.
authors: Institute of Parenteral Nutrition and Enteral Nutrition, Department of Surgery, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Yishan Rd. 600, Shanghai 200233, China. hlqin@sjtu.edu.cn
authors: Huan-Long
authors: HL
authors: Qin
authors: Zhen-Dong
authors: ZD
authors: Su
authors: Lei-Guang
authors: LG
authors: Hu
authors: Zai-Xian
authors: ZX
authors: Ding
authors: Qing-Tian
authors: QT
authors: Lin
mesh_terms: D000208:Acute Disease; D000681:Amylases / Q000378:metabolism*; D000818:Animals; D002401:Cathepsin B / Q000378:metabolism*; D003902:Detergents; D004195:Disease Models, Animal; D004285:Dogs; D003576:Electron Transport Complex IV / Q000378:metabolism*; D004750:Enteral Nutrition / Q000379:methods*; D008247:Lysosomes / Q000201:enzymology; D010179:Pancreas / Q000201:enzymology / Q000473:pathology / Q000648:ultrastructure; D010195:Pancreatitis / Q000139:chemically induced / Q000201:enzymology* / Q000473:pathology; D010288:Parenteral Nutrition / Q000379:methods*; D022142:Secretory Vesicles / Q000201:enzymology; D013656:Taurocholic Acid
chemical_list: D003902:Detergents; D013656:Taurocholic Acid; D003576:Electron Transport Complex IV; D000681:Amylases; D002401:Cathepsin B