Electrochemical behavior and square wave voltammetric determination of aristolochic acid-I.
Electroanalytical procedure for the determination of nephrotoxic aristolochic acid-I in the medicinal plant has been developed in the presence of potential interferences of lead and cadmium by square wave voltametry (SWV). Among the phosphate buffers of pH values at 5.0, 6.1, 6.5 and 7.0, the phosphate buffers of pH 6.1 yielded the most accurate analysis of AA-I in the presence of Pb2+ and Cd2+; Pb2+ was precipitated as Pb(HPO4) and did not appear in the SW voltammogram, while Cd2+ appeared at -0.564 V which was well resolved from AA-I at -0.416 V. When the Ip of AA-I was plotted vs. concentrations between 1.67 x 10(-8) M and 1.67 x 10(-6) M in the presence of Pb2+ and Cd2+, a linear calibration curve was obtained with a slope of 6 x 10(8) nA/M and a correlation coefficient of 0.9999. The present method was applied to determine AA in the dried natural products of Aristolochia contorta Bunge; Total AA in the dried root and the ripe fructus of Aristolochia contorta Bunge were found as 25 +/- 1 microg/g and 85 +/- 3 microg/g, respectively.
Kim
Mi Sun
MS
Department of Chemistry, Sangmyung University, Seoul 110-743, Korea.
Hahn
Younghee
Y
D030022:Aristolochia / Q000737:chemistry*; D034341:Aristolochic Acids / Q000032:analysis*; D002138:Calibration; D004563:Electrochemistry; D004566:Electrodes; D005638:Fruit / Q000737:chemistry; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D034341:Aristolochic Acids; D010936:Plant Extracts; C000228:aristolochic acid I