title: Retinoic acid-inducible gene-I mediates RANTES/CCL5 expression in U373MG human astrocytoma cells stimulated with double-stranded RNA.
abstract: Retinoic acid-inducible gene-I (RIG-I) mediates part of the cell signaling in response to viral infection. Polyinosinic-polycytidilic acid (poly IC) is a synthetic double-stranded RNA (dsRNA) and mimics viral infection when applied to cell cultures. The CC chemokine, RANTES (regulated on activation, normal T-cell expressed and secreted), is a potent attractant for inflammatory cells such as memory T-lymphocytes, monocytes and eosinophils. In the present study, we demonstrated that poly IC enhances the expression of RIG-I in U373MG human astrocytoma cells. The RNA interference of RIG-I resulted in the suppression of the poly IC-induced RANTES expression. Pretreatment of the cells with SB203580, an inhibitor of p38 mitogen-activated protein kinase, and dexamethasone inhibited the poly IC-induced expression of RIG-I. Furthermore, poly IC upregulated RIG-I in normal human astrocytes in culture and the in vivo injection of poly IC into the striatum of the mouse brain induced the expression of RIG-I in astrocytes. We conclude that RIG-I may be involved in immune reactions against viral infection, at least in part, through the regulation of RANTES expression in astrocytes.
authors: Department of Vascular Biology, Institute of Brain Science, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan. hidemiyo@cc.hirosaki-u.ac.jp
authors: Hidemi
authors: H
authors: Yoshida
authors: Tadaatsu
authors: T
authors: Imaizumi
authors: Sung Joong
authors: SJ
authors: Lee
authors: Kunikazu
authors: K
authors: Tanji
authors: Hirotaka
authors: H
authors: Sakaki
authors: Tomoh
authors: T
authors: Matsumiya
authors: Akira
authors: A
authors: Ishikawa
authors: Kageaki
authors: K
authors: Taima
authors: Ei
authors: E
authors: Yuzawa
authors: Fumiaki
authors: F
authors: Mori
authors: Koichi
authors: K
authors: Wakabayashi
authors: Hiroto
authors: H
authors: Kimura
authors: Kei
authors: K
authors: Satoh
mesh_terms: D001254:Astrocytoma / Q000378:metabolism*; D045744:Cell Line, Tumor; D018946:Chemokine CCL5 / Q000235:genetics / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression / Q000187:drug effects*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D007369:Interferon Inducers / Q000494:pharmacology; D011070:Poly I-C / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D012330:RNA, Double-Stranded / Q000494:pharmacology*; D013997:Time Factors; D015534:Trans-Activators; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D018946:Chemokine CCL5; D004791:Enzyme Inhibitors; D005904:Glial Fibrillary Acidic Protein; D007093:Imidazoles; D007369:Interferon Inducers; D011725:Pyridines; C431552:RAI1 protein, human; D012330:RNA, Double-Stranded; D015534:Trans-Activators; D014157:Transcription Factors; D011070:Poly I-C; C093642:SB 203580