Characterization of serum and mucosal antibody responses in white sturgeon (Acipenser transmontanus Richardson) following immunization with WSIV and a protein hapten antigen.
Serum and cutaneous mucus antibodies were monitored in white sturgeon for 15 weeks following intraperitoneal immunization. Ten fish were immunized (50 microg) with white sturgeon iridovirus (WSIV) or white sturgeon gonad (WSGO) tissue culture cells emulsified with or without FCA. An additional group was immunized with FITC:KLH+FCA. Fish were booster immunized at 6 weeks. Fish immunized with FITC:KLH+FCA produced significant serum antibodies to FITC by 6 weeks and this response peaked at 12 weeks (average titer 31,000). Mucosal antibodies to FITC were first detected at 12 weeks and significantly elevated by 15 weeks (average titer 18). Anti-WSIV antibody titers were detected in the serum by 9 weeks in fish immunized with WSIV and WSIV+FCA, but only a small number responded to immunization. At 15 weeks, four fish immunized with WSIV produced serum antibodies (average titer 838) and one fish immunized with WSIV+FCA had a serum titer of 1600. Mucosal anti-WSIV antibody titers of 8 and 16 were observed in two fish from the WSIV group at 12 weeks while four different fish from this group responded at 15 weeks (average titer 4). Western Blot using a monoclonal antibody confirmed immunoglobulin in mucus, and specificity to WSIV was further demonstrated by immunocytochemistry using serum from fish immunized with WSIV. Specific antibody was not detected in mucus of fish immunized with WSIV+FCA, WSGO, or WSGO+FCA. Collectively, these experiments demonstrate that white sturgeon can generate a specific antibody response following immunization, and is the first report showing mucosal immunoglobulin is present in this species.
Drennan
John D
JD
Department of Fish and Wildlife Resources and the Aquaculture Research Institute, University of Idaho, 6th and Line Street, CRN Building, Moscow, ID 83844-1136, USA.
Lapatra
Scott E
SE
Swan
Christine M
CM
Ireland
Sue
S
Cain
Kenneth D
KD
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology / Q000378:metabolism; D000917:Antibody Formation / Q000276:immunology; D000956:Antigens, Viral / Q000008:administration & dosage / Q000276:immunology*; D015153:Blotting, Western; D002460:Cell Line; D004266:DNA Virus Infections / Q000097:blood / Q000276:immunology / Q000517:prevention & control; D005393:Fish Diseases / Q000097:blood / Q000276:immunology / Q000517:prevention & control; D005399:Fishes / Q000097:blood / Q000276:immunology*; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D006066:Gonads / Q000166:cytology / Q000821:virology; D006241:Haptens / Q000008:administration & dosage / Q000276:immunology*; D018928:Immunity, Mucosal / Q000276:immunology*; D007114:Immunization / Q000662:veterinary*; D007150:Immunohistochemistry; D017994:Iridovirus / Q000276:immunology*; D009132:Muscles / Q000276:immunology; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D006241:Haptens; D015164:Vaccines, Inactivated; D014765:Viral Vaccines; D016650:Fluorescein-5-isothiocyanate