title: Modulation of mucosal permeability by vasoactive intestinal peptide or lidocaine affects the adjustment of luminal hypotonicity in rat duodenum.
abstract: OBJECTIVE
  To examine whether modulation of paracellular solute permeability affects the capability of the duodenum to adjust luminal osmolality.
  
  
  METHODS
  Proximal duodenum was perfused with a hypotonic NaCl solution and effects on paracellular permeability to (51)Cr-EDTA, motility, anion secretion, net fluid flux and perfusate osmolality determined in anaesthetized rats in the absence and presence of the COX-2 inhibitor parecoxib. Vasoactive intestinal peptide (VIP) was used to reduce and lidocaine to augment the hypotonicity-induced increase in paracellular permeability.
  
  
  RESULTS
  Luminal hypotonicity slightly increased paracellular permeability in control animals. Parecoxib induced motility, increased electrolyte and fluid secretion, potentiated the hypotonicity-induced rise in paracellular permeability and enhanced the capability to adjust luminal osmolality. VIP, given to control animals stimulated electrolyte and fluid secretion and augmented the capability to adjust luminal osmolality. Administration of VIP to parecoxib-treated animals increased secretion further, markedly reduced the hypotonicity-induced increase in permeability but did not change the osmolality-adjusting capability. Luminal lidocaine potentiated the hypotonicity-induced increase in permeability, reduced the hypotonicity-induced net fluid absorption and the osmolality-adjusting capability was 50% greater than in controls. Lidocaine, given to parecoxib-treated animals potentiated the hypotonicity-induced increase in permeability, reduced the hypotonicity-induced net fluid absorption but did not change the osmolality-adjusting capability.
  
  
  CONCLUSIONS
  Vasoactive intestinal peptide reduces the osmolality-adjusting capacity of the duodenum by inhibiting paracellular solute permeability but improves this capacity by stimulating active electrolyte and fluid secretion. In contrast, lidocaine improves the osmolality-adjusting capability by augmenting paracellular solute transport but depresses it by reducing the hypotonicity-induced net fluid absorption.
authors: Department of Neuroscience, Division of Physiology, Biomedical Center, Uppsala University, Uppsala, Sweden. olof.nylander@fysiologi.uu.se
authors: O
authors: O
authors: Nylander
authors: M
authors: M
authors: Sjöblom
mesh_terms: D000779:Anesthetics, Local / Q000008:administration & dosage*; D000818:Animals; D001639:Bicarbonates / Q000378:metabolism; D001692:Biological Transport / Q000502:physiology; D001794:Blood Pressure / Q000502:physiology; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology; D016861:Cyclooxygenase Inhibitors / Q000008:administration & dosage; D004386:Duodenum / Q000187:drug effects / Q000502:physiology*; D005765:Gastrointestinal Agents / Q000008:administration & dosage*; D005769:Gastrointestinal Motility / Q000502:physiology; D007262:Infusions, Intravenous; D007408:Intestinal Absorption / Q000187:drug effects; D007413:Intestinal Mucosa / Q000187:drug effects / Q000502:physiology*; D007555:Isoxazoles / Q000008:administration & dosage; D008012:Lidocaine / Q000008:administration & dosage*; D008297:Male; D009994:Osmolar Concentration; D010477:Perfusion; D051381:Rats; D011917:Rats, Inbred Lew; D014660:Vasoactive Intestinal Peptide / Q000008:administration & dosage*
chemical_list: D000779:Anesthetics, Local; D001639:Bicarbonates; D016861:Cyclooxygenase Inhibitors; D005765:Gastrointestinal Agents; D007555:Isoxazoles; D014660:Vasoactive Intestinal Peptide; D008012:Lidocaine; C409945:parecoxib