GSK3 alpha and GSK3 beta are necessary for axon formation.
The mechanisms that underlie axon formation are still poorly understood. GSK3 has been recently implicated in establishing the axon and in its elongation. We have used four different GSK3 inhibitors to determine the role of GSK3 activity in hippocampal neurons at different periods of time. Inhibition of GSK3 activity impairs axon formation. The "critical period" of this activity of GSK3 is at least the first 24h since afterwards the inhibition of GSK3 does not compromise the process of elongation, although it exacerbates axon branching. Moreover, interference RNAs impeding the expression of the GSK3 alpha or beta isoforms in hippocampal neurons prevents an axon from forming.
Garrido
Juan José
JJ
Centro de Biología Molecular Severo Ochoa, Facultad de Ciencias, CSIC-Universidad Autónoma de Madrid, Cantoblanco,Madrid, Spain.
Simón
Diana
D
Varea
Olga
O
Wandosell
Francisco
F
D000818:Animals; D001369:Axons / Q000201:enzymology / Q000502:physiology*; D016764:Cell Polarity / Q000235:genetics; D002478:Cells, Cultured; D038362:Glycogen Synthase Kinase 3 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D000071679:Glycogen Synthase Kinase 3 beta; D006624:Hippocampus / Q000166:cytology / Q000201:enzymology; D051379:Mice; D008815:Mice, Inbred Strains; D009474:Neurons / Q000201:enzymology
D000071679:Glycogen Synthase Kinase 3 beta; D038362:Glycogen Synthase Kinase 3; C448021:glycogen synthase kinase 3 alpha