title: Different obscurin isoforms localize to distinct sites at sarcomeres.
abstract: We used four antibodies to regions of obscurin isoforms A and B, encoded by the obscurin gene, to investigate the location of these proteins in skeletal myofibers at resting and stretched lengths. Obscurin A ( approximately 800 kDa) which was recognized by antibodies generated to the N-terminal, Rho-GEF, and the non-modular C-terminal domain that lacks the kinase-like domains, localizes at the level of the M-band. Obscurin B ( approximately 900 kDa) which has the N-terminal, Rho-GEF, and the C-terminal kinase-like domains, localizes at the level of the A/I junction. Additional isoforms, which lack one or more of these epitopes, are present at the Z-disk and Z/I junction.
authors: Department of Physiology, University of Maryland School of Medicine, 655 W. Baltimore St, MD 21201, USA.
authors: Amber L
authors: AL
authors: Bowman
authors: Aikaterini
authors: A
authors: Kontrogianni-Konstantopoulos
authors: Sara S
authors: SS
authors: Hirsch
authors: Sarah B
authors: SB
authors: Geisler
authors: Hugo
authors: H
authors: Gonzalez-Serratos
authors: Mark W
authors: MW
authors: Russell
authors: Robert J
authors: RJ
authors: Bloch
mesh_terms: D000818:Animals; D009124:Muscle Proteins / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry*; D020033:Protein Isoforms / Q000032:analysis; D012518:Sarcomeres / Q000737:chemistry*
chemical_list: D009124:Muscle Proteins; D020033:Protein Isoforms