Abnormal expression, highly efficient detection and novel truncations of midkine in human tumors, cancers and cell lines.
We detected aberrant Midkine (MK) expressions in human insulinoma and pancreatic cancer tissues by immunohistochemistry, revealing its potential role in tumorigenesis/carcinogenesis. With a nested-touchdown PCR program we were able to detect the tMK in all human tumor/cancer tissues and cancer/tumor cell lines. Detection of MK in the peripheral cells and precancerous lesions implies its potential for early cancer/tumor diagnosis. Furthermore, we have discovered two novel truncations of the MK, tMKB and tMKC, respectively, in the disease specimens. Our data not only provide an efficient methodology potentially for clinical application but also shed light on the molecular mechanism underlying the role for MK in tumorigenesis/carcinogenesis.
Tao
Ping
P
Department of Biomedical Sciences, Xiamen University, Xiamen City, Fujian Province 361005, China. tapoing@yahoo.com.cn
Xu
Donghui
D
Lin
Shuibin
S
Ouyang
Gao-Liang
GL
Chang
Youde
Y
Chen
Qin
Q
Yuan
Yuanyang
Y
Zhuo
Xinming
X
Luo
Qicong
Q
Li
Jie
J
Li
Baoan
B
Ruan
Lingjuan
L
Li
Qifu
Q
Li
Zhixing
Z
D000328:Adult; D000595:Amino Acid Sequence; D045744:Cell Line, Tumor; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007340:Insulinoma / Q000235:genetics* / Q000378:metabolism; D000078792:Midkine; D008969:Molecular Sequence Data; D009154:Mutation; D010179:Pancreas / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D016207:Cytokines; D000078792:Midkine