title: Efficient multicistronic expression of a transgene in human embryonic stem cells.
abstract: The applicability of human embryonic stem cells (hESCs) will be greatly enhanced by techniques that permit efficient genetic modification with multiple transgenes. We report here on single-promoter-driven foot-and-mouth disease virus segment 2A-mediated multicistronic expression of a transgene in hESCs. Efficient multicistronic expression of the transgene was permitted by 2A-mediated separation with almost the same amounts of encoded proteins in hESC. In addition, the multicistronic protein expression was successful in hESC-derived differentiated cells in in vivo and in vitro differentiation assays. This technology may be a significant advance in the genetic engineering of hESCs and hESC-derived cells for purposes that require the reliable expression of multiple transgenes. Disclosure of potential conflicts of interest is found at the end of this article.
authors: Laboratory of Embryonic Stem Cell Research, Stem Cell Research Center, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawaharacho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
authors: Kouichi
authors: K
authors: Hasegawa
authors: Aaron B
authors: AB
authors: Cowan
authors: Norio
authors: N
authors: Nakatsuji
authors: Hirofumi
authors: H
authors: Suemori
mesh_terms: D000818:Animals; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D051379:Mice; D019076:Transgenes / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins; C057421:virus protein 2A