title: Impact of preservation solution on the extent of blood-air barrier damage and edema formation in experimental lung transplantation.
abstract: A major aim in lung transplantation is to prevent the loss of structural integrity due to ischemia and reperfusion (I/R) injury. Preservation solutions protect the lung against I/R injury to a variable extent. We compared the influence of two extracellular-type preservation solutions (Perfadex, or PX, and Celsior, or CE) on the morphological alterations induced by I/R. Pigs were randomly assigned to sham (n = 4), PX (n = 5), or CE (n = 2) group. After flush perfusion with PX or CE, donor lungs were excised and stored for 27 hr at 4 degrees C. The left donor lung was implanted into the recipient, reperfused for 6 hr, and, afterward, prepared for light and electron microscopy. Intra-alveolar, septal, and peribronchovascular edema as well as the integrity of the blood-air barrier were determined stereologically. Intra-alveolar edema was more pronounced in CE (219.80 +/- 207.55 ml) than in PX (31.46 +/- 15.75 ml). Peribronchovascular (sham: 13.20 +/- 4.99 ml; PX: 15.57 +/- 5.53 ml; CE: 31.56 +/- 5.78 ml) and septal edema (thickness of alveolar septal interstitium, sham: 98 +/- 33 nm; PX: 84 +/- 8 nm; CE: 249 +/- 85 nm) were only found in CE. The blood-air barrier was similarly well preserved in sham and PX but showed larger areas of swollen and fragmented epithelium or endothelium in CE. The present study shows that Perfadex effectively prevents intra-alveolar, septal, and peribronchovascular edema formation as well as injury of the blood-air barrier during I/R. Celsior was not effective in preserving the lung from morphological I/R injury.
authors: Institute of Anatomy, University of Bern, Bern, Switzerland. christian.muehlfeld@ana.unibe.ch
authors: Christian
authors: C
authors: Mühlfeld
authors: Katharina
authors: K
authors: Müller
authors: Lars-Peder
authors: LP
authors: Pallesen
authors: Tim
authors: T
authors: Sandhaus
authors: Navis
authors: N
authors: Madershahian
authors: Joachim
authors: J
authors: Richter
authors: Thorsten
authors: T
authors: Wahlers
authors: Thorsten
authors: T
authors: Wittwer
authors: Matthias
authors: M
authors: Ochs
mesh_terms: D000818:Animals; D015824:Blood-Air Barrier / Q000187:drug effects* / Q000648:ultrastructure; D002951:Citrates / Q000494:pharmacology; D004187:Disaccharides / Q000494:pharmacology; D004573:Electrolytes / Q000494:pharmacology; D005971:Glutamates / Q000494:pharmacology; D005978:Glutathione / Q000494:pharmacology; D006639:Histidine / Q000494:pharmacology; D008168:Lung / Q000098:blood supply / Q000187:drug effects* / Q000648:ultrastructure; D016040:Lung Transplantation / Q000379:methods*; D008353:Mannitol / Q000494:pharmacology; D046529:Microscopy, Electron, Transmission; D023421:Models, Animal; D009926:Organ Preservation / Q000009:adverse effects / Q000379:methods*; D019188:Organ Preservation Solutions / Q000494:pharmacology* / Q000627:therapeutic use; D011654:Pulmonary Edema / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D011897:Random Allocation; D015427:Reperfusion Injury / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D013552:Swine
chemical_list: C088453:Celsior; D002951:Citrates; D004187:Disaccharides; D004573:Electrolytes; D005971:Glutamates; D019188:Organ Preservation Solutions; C070005:Perfadex; D008353:Mannitol; D006639:Histidine; D005978:Glutathione