Screening of beta-fructofuranosidase-producing microorganisms and effect of pH and temperature on enzymatic rate.
Seventeen different strains of filamentous fungi were grown in batch cultures to compare their abilities for the production of beta-fructofuranosidase. Three of them, Aspergillus oryzae IPT-301, Aspergillus niger ATCC 20611 and strain IPT-615, showed high production with total fructosyltransferase activity higher than 12,500 units l(-1). In addition, the beta-fructofuranosidases of those strains have a high fructosyltransferase activity-to-hydrolytic activity ratio. The temperature and pH effects on the sucrose-beta-fructofuranosidase reaction rate were studied using a 2(2) factorial experimental design. The comparative analysis of the tested variable coefficients shows that the variable pH contributes mostly to the changes in the fructosyltransferase and hydrolytic rates and in the V (t)/V (h) ratio. At 40 and 50 degrees C, there were no significant differences between the fructosyltransferase and hydrolytic velocities of these enzymes.
Fernandez
Rubén Cuervo
RC
Instituto Cubano de Investigaciones Azucareras-ICINAZ, Carretera al Central Manuel M Prieto Km 21/2, Boyeros, Havana, Cuba.
Ottoni
Cristiane Angélica
CA
da Silva
Elda Sabino
ES
Matsubara
Rosa Mitiko Saito
RM
Carter
José Márcio
JM
Magossi
Luis Roberto
LR
Wada
Maria Alice Alves
MA
de Andrade Rodrigues
Maria Filomena
MF
Maresma
Beatriz Guilarte
BG
Maiorano
Alfredo Eduardo
AE
D001234:Aspergillus niger / Q000201:enzymology; D001236:Aspergillus oryzae / Q000201:enzymology; D005658:Fungi / Q000145:classification / Q000201:enzymology*; D006602:Hexosyltransferases / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology / Q000379:methods*; D009844:Oligosaccharides / Q000378:metabolism; D031786:Saccharum / Q000254:growth & development*; D012988:Soil Microbiology; D013696:Temperature; D043324:beta-Fructofuranosidase / Q000378:metabolism*; D001426:Bacterial Proteins
D006602:Hexosyltransferases; D009844:Oligosaccharides; D043324:beta-Fructofuranosidase; C116580:fructooligosaccharide; C021451:inulosucrase; D001426:Bacterial Proteins