Experimental design and capillary electrophoresis for simultaneous analysis of arbutin, kojic acid and hydroquinone in cosmetics.
A statistical experimental design was used to optimize one micellar electrokinetic capillary electrophoresis (MEKC) for simultaneous analysis of arbutin (AR), kojic acid (KA) and hydroquinone (HQ). Untreated fused-silica capillaries were operated using a phosphate buffer (20mM, pH 6.5) under 20 kV and detection at 200 nm. Quantitative linear ranges were 20-200 microg/ml for AR, 20-100 microg/ml for KA and 8-80 microg/ml for HQ with correlation coefficients >or=0.9994. R.S.D. and R.E. were less than 3.0% for the intra-day and inter-day analysis, and all recoveries were greater than 99%. Our method was applied to assay commercial cosmetics. The results were within the labeled amount of 99.6-102.5%.
Lin
Yi-Hui
YH
Graduate Institute of Pharmaceutical Sciences, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
Yang
Yi-Hsin
YH
Wu
Shou-Mei
SM
D001104:Arbutin / Q000032:analysis*; D002021:Buffers; D003358:Cosmetics / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D006873:Hydroquinones / Q000032:analysis*; D010710:Phosphates / Q000737:chemistry; D011753:Pyrones / Q000032:analysis*; D012015:Reference Standards; D012044:Regression Analysis; D015203:Reproducibility of Results; D012107:Research Design; D014508:Urea; D014867:Water / Q000737:chemistry
D002021:Buffers; D003358:Cosmetics; D006873:Hydroquinones; D010710:Phosphates; D011753:Pyrones; D014867:Water; C011890:kojic acid; D014508:Urea; D001104:Arbutin; C031927:hydroquinone