title: Progress in the methodological strategies for the detection in real samples of desmosine and isodesmosine, two biological markers of elastin degradation.
abstract: Desmosines are crosslinking amino acids unique to mature elastin in humans. Owing to this unicity, they have been discussed as potentially attractive indicators of connective tissue disorders whose clinical manifestations are mostly the result of elastin degradation. This review covers advances in immunochemical, chromatographic, and electrophoretic procedures applied in the last 25 years to detect and quantitate these crosslinksin a variety of biological samples. Recent applications of CE with LIF detection (CE-LIF) for investigating the content of desmosines in different fluids will also be discussed.
authors: Dipartimento di Biochimica "A. Castellani", Università di Pavia, Italy.
authors: Simona
authors: S
authors: Viglio
authors: Laura
authors: L
authors: Annovazzi
authors: Maurizio
authors: M
authors: Luisetti
authors: Jan
authors: J
authors: Stolk
authors: Begoña
authors: B
authors: Casado
authors: Paolo
authors: P
authors: Iadarola
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D003895:Desmosine / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D004549:Elastin / Q000378:metabolism*; D006801:Humans; D007120:Immunochemistry; D007524:Isodesmosine / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D015415:Biomarkers; D003895:Desmosine; D004549:Elastin; D007524:Isodesmosine