title: Panmicrobial oligonucleotide array for diagnosis of infectious diseases.
abstract: To facilitate rapid, unbiased, differential diagnosis of infectious diseases, we designed GreeneChipPm, a panmicrobial microarray comprising 29,455 sixty-mer oligonucleotide probes for vertebrate viruses, bacteria, fungi, and parasites. Methods for nucleic acid preparation, random primed PCR amplification, and labeling were optimized to allow the sensitivity required for application with nucleic acid extracted from clinical materials and cultured isolates. Analysis of nasopharyngeal aspirates, blood, urine, and tissue from persons with various infectious diseases confirmed the presence of viruses and bacteria identified by other methods, and implicated Plasmodium falciparum in an unexplained fatal case of hemorrhagic feverlike disease during the Marburg hemorrhagic fever outbreak in Angola in 2004-2005.
authors: Columbia University, New York, New York 10032, USA.
authors: Gustavo
authors: G
authors: Palacios
authors: Phenix-lan
authors: PL
authors: Quan
authors: Omar J
authors: OJ
authors: Jabado
authors: Sean
authors: S
authors: Conlan
authors: David L
authors: DL
authors: Hirschberg
authors: Yang
authors: Y
authors: Liu
authors: Junhui
authors: J
authors: Zhai
authors: Neil
authors: N
authors: Renwick
authors: Jeffrey
authors: J
authors: Hui
authors: Hedi
authors: H
authors: Hegyi
authors: Allen
authors: A
authors: Grolla
authors: James E
authors: JE
authors: Strong
authors: Jonathan S
authors: JS
authors: Towner
authors: Thomas W
authors: TW
authors: Geisbert
authors: Peter B
authors: PB
authors: Jahrling
authors: Cornelia
authors: C
authors: Büchen-Osmond
authors: Heinz
authors: H
authors: Ellerbrok
authors: Maria Paz
authors: MP
authors: Sanchez-Seco
authors: Yves
authors: Y
authors: Lussier
authors: Pierre
authors: P
authors: Formenty
authors: M Stuart T
authors: MS
authors: Nichol
authors: Heinz
authors: H
authors: Feldmann
authors: Thomas
authors: T
authors: Briese
authors: W Ian
authors: WI
authors: Lipkin
mesh_terms: D003141:Communicable Diseases / Q000175:diagnosis* / Q000821:virology; D004196:Disease Outbreaks; D017809:Fatal Outcome; D006801:Humans; D016778:Malaria, Falciparum / Q000175:diagnosis; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods*; D010802:Phylogeny; D012680:Sensitivity and Specificity; D014777:Virus Diseases / Q000175:diagnosis* / Q000821:virology