Evaluation of antioxidant effect of extracts of Symphyopappus casarettoi.
The ethanolic extract of Symphyopappus casarettoi syn. Eupatorium casarettoi was partionated in hexanic, chloroformic and methanolic fractions. Extract and fractions were tested for their antioxidant activity in vitro and ex vivo assays. The methanolic fraction showed a higher antioxidant potential compared to the others fractions, which was correlated with its total phenol content. In addition, the ethanolic extract and the methanolic fraction attenuated ex vivo iron-induced cell death, quantified by lactate dehydrogenase leakage, and effectively protected against lipid damage induced by iron. These findings suggest that the ethanolic extract of S. casarettoi inflorescence and its methanolic fraction have in vitro and ex vivo antioxidant properties.
Netto Benetti
Mara Regina
MR
Curso de Pós-Graduação em Ciências Biológicas-Bioquímica, Instituto de Ciências Básicas de Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600 (anexo), Porto Alegre, RS, CEP: 90035-003, Brazil. marare1@hotmail.com
Rudnicki
Martina
M
Zanotto-Filho
Alfeu
A
de Oliveira
Marcos Roberto
MR
Kurek
Andréa Gisiane
AG
Coitinho
Adriana
A
Schwartsmann
Gilberto
G
Moreira
José Cláudio Fonseca
JC
D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects; D019659:Asteraceae; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007501:Iron; D015227:Lipid Peroxidation / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D017392:Thiobarbituric Acid Reactive Substances / Q000737:chemistry
D000975:Antioxidants; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; D007501:Iron