[Cloning and characterization of genes differentially expressed in human dental pulp cells and gingival fibroblasts].
OBJECTIVE
To study the biological properties of human dental pulp cells (HDPC) by cloning and analysis of genes differentially expressed in HDPC in comparison with human gingival fibroblasts (HGF).


METHODS
HDPC and HGF were cultured and identified by immunocytochemistry. HPDC and HGF subtractive cDNA library was established by PCR-based modified subtractive hybridization, genes differentially expressed by HPDC were cloned, sequenced and compared to find homogeneous sequence in GenBank by BLAST.


RESULTS
Cloning and sequencing analysis indicate 12 genes differentially expressed were obtained, in which two were unknown genes. Among the 10 known genes, 4 were related to signal transduction, 2 were related to trans-membrane transportation (both cell membrane and nuclear membrane), and 2 were related to RNA splicing mechanisms.


CONCLUSIONS
The biological properties of HPDC are determined by the differential expression of some genes and the growth and differentiation of HPDC are associated to the dynamic protein synthesis and secretion activities of the cell.
Wang
Zhong-dong
ZD
Dept. of Endodontics, Guanghua College of Stomatology, Sun Yat-Sen University, Guangzhou 510055, China.
Wu
Ji-nan
JN
Zhou
Lin
L
Ling
Jun-qi
JQ
Guo
Xi-min
XM
Xiao
Ming-zhen
MZ
Zhu
Feng
F
Pu
Qin
Q
Chai
Yu-bo
YB
Zhao
Zhong-liang
ZL
D003001:Cloning, Molecular; D019976:Cloning, Organism; D003782:Dental Pulp; D005347:Fibroblasts; D015723:Gene Library; D005881:Gingiva; D006801:Humans; D016133:Polymerase Chain Reaction