Manipulating the manipulators: advances in parasitic helminth transgenesis and RNAi.
Because tropical medicine and parasitology research has moved into the postgenomic era, an enormous amount of gene sequence information for parasitic helminths is now accumulating. These sequences undoubtedly hold information that can be used for new interventions and control. However, to exploit the new resource, methods for gene manipulation and transformation of parasitic worms are needed. Until recently, gene manipulation approaches had not been seriously addressed. This situation is now changing in response to the availability of genome sequences and other advances. In this article, we review advances in the transgenesis and gene silencing of parasitic worms.
Kalinna
Bernd H
BH
Centre for Animal Biotechnology, Faculty of Veterinary Science, The University of Melbourne, Parkville, 3010 VIC, Australia. bernd.kalinna@unimelb.edu.au
Brindley
Paul J
PJ
D000818:Animals; D005786:Gene Expression Regulation; D020868:Gene Silencing; D017238:Genes, Helminth; D005821:Genetic Techniques; D006376:Helminths / Q000235:genetics*; D034622:RNA Interference; D018510:RNA, Helminth / Q000235:genetics; D012547:Schistosoma / Q000235:genetics; D014162:Transfection
D018510:RNA, Helminth