Local application of a collagen type I/hyaluronate matrix and growth and differentiation factor 5 influences the closure of osteochondral defects in a minipig model by enchondral ossification.
This pilot study evaluated the effect of growth and differentiation factor-5 (rhGDF-5) combined with a collagen type I/hyaluronate matrix (c/h) on osteochondral defect repair in a minipig model. Defects created in both medial femoral condyles of 20 minipigs were treated with c/h (n = 10), c/h + rhGDF-5 (n = 10) or were left empty. After 3 and 12 months, five animals of each group were sacrificed. Evaluation included macroscopic and histological scoring and quantitative histomorphometry of synthesized bone. C/h and c/h + rhGDF-5 treatment increased trabecular bone formation in the upper third of the defect compared to empty controls, showing significance for c/h + rhGDF-5 (p = 0.05) but not between c/h and c/h + rhGDF-5 treatment. Cartilage regeneration and macroscopic outcome were not improved by c/h or c/h + rhGDF-5 treatment. Since c/h remnants were seen even one year postoperatively in the defect, possibly inhibiting further bone and cartilage healing, other matrices in combination with rhGDF-5 may provide further improvement in osteochondral defect treatment.
Jung
Martin
M
Department of Orthopaedic Surgery, University of Heidelberg, Heidelberg, Germany. martin.jung@ok.uni-heidelberg.de
Tuischer
Jens Sascha
JS
Sergi
Consolato
C
Gotterbarm
Tobias
T
Pohl
Jens
J
Richter
Wiltrud
W
Simank
Hans-Georg
HG
D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D001842:Bone and Bones / Q000187:drug effects / Q000502:physiology; D002356:Cartilage / Q000187:drug effects / Q000502:physiology; D024042:Collagen Type I / Q000008:administration & dosage / Q000494:pharmacology*; D004195:Disease Models, Animal; D005260:Female; D055428:Growth Differentiation Factor 5; D006820:Hyaluronic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D010012:Osteogenesis; D010865:Pilot Projects; D011897:Random Allocation; D013552:Swine; D013556:Swine, Miniature
D019485:Bone Morphogenetic Proteins; D024042:Collagen Type I; D055428:Growth Differentiation Factor 5; D006820:Hyaluronic Acid