Hybrid titania-aminosilane platforms evaluated with human mesenchymal stem cells.
The properties of hybrid aminopropyltriethoxysilane-tetraisopropylorthotitanate (APTS-TIPT) platforms prepared by a sol-gel route have been explored, and their biocompatibility was assayed after culture of human mesenchymal stem cells (hMSCs). The organic content of this material was observed to be preferably surface-oriented as indicated by microanalytical techniques. Furthermore, the surface showed characteristic amino-silane bands when explored by Raman spectroscopy as well as indications of silane and titanate condensation. Surface activity of the amino groups was probed by ultraviolet-visible spectroscopy imine derivatization and chemical force spectroscopy, showing a pH-dependent surface charge-induced potential. hMSCs cultured onto these surfaces showed relevant differences with respect to their behavior on gelatin-coated glass plates. Even if with a lower proliferative rate than controls, the cells develop long cytosolic prolongations in osteogenic differentiation medium, thus, supporting the idea of an APTS-TIPT stimulated process.
Manso-Silván
M
M
Departamento de Física Aplicada C-XII-104, Universidad Autónoma de Madrid, 28049 Madrid, Spain. miguel.manso@uam.es
Rodríguez-Navas
C
C
Arroyo-Hernández
M
M
López-Elvira
E
E
Gago
R
R
Vázquez
L
L
Agulló-Rueda
F
F
Climent
A
A
Martínez-Duart
J M
JM
García-Ruiz
J P
JP
D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism*; D002478:Cells, Cultured; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007097:Imines / Q000737:chemistry; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D012821:Silanes / Q000737:chemistry / Q000378:metabolism*; D014025:Titanium / Q000737:chemistry / Q000378:metabolism*
D001672:Biocompatible Materials; D007097:Imines; D012821:Silanes; D014025:Titanium