title: Determination of verapamil hydrochloride with 12-tungstophosphoric acid by resonance Rayleigh scattering method coupled to flow injection system.
abstract: A flow injection analysis (FIA) method coupled to resonance Rayleigh scattering (RRS) detection for the determination of verapamil hydrochloride (VP) was proposed. In pH 1.0 acidic medium, 12-tungstophosphoric acid (TP) reacted with VP to form an ion-association complex, which resulted in a significant enhancement of RRS intensity. The maximum scattering peak was located at 293 nm. RRS intensity was proportional to the concentration of VP in the range of 0.017-13.0 microg mL(-1), and the detection limit (3sigma) was 5.1 ng mL(-1). The proposed method exhibited satisfactory reproducibility with a relative standard derivation (R.S.D.) of 2.1% for 11 successive determinations of 5.0 microg mL(-1) VP. Therefore, a novel method for the determination of VP by FIA-RRS was developed. The optimum reaction conditions and the parameters of the FIA operation such as flow rate, injection volume, reactor length, and so on had been optimized in this paper. The present method had been applied to the determination of VP in serum samples and pharmaceuticals with satisfactory results. The maximal sample throughput in the optimized system was 80 h(-1).
authors: School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors: Dongpo
authors: D
authors: Xu
authors: Shaopu
authors: S
authors: Liu
authors: Zhongfang
authors: Z
authors: Liu
authors: Xiaoli
authors: X
authors: Hu
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002626:Chemistry, Pharmaceutical / Q000379:methods; D017022:Flow Injection Analysis / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007477:Ions; D008958:Models, Molecular; D010756:Phosphoric Acids / Q000032:analysis*; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence; D014414:Tungsten / Q000737:chemistry*; D017973:Tungsten Compounds / Q000032:analysis*; D014700:Verapamil / Q000032:analysis*
chemical_list: C519880:12-tungstophosphoric acid; D007477:Ions; D010756:Phosphoric Acids; D017973:Tungsten Compounds; D014700:Verapamil; C030242:phosphoric acid; D014414:Tungsten