A nuclear magnetic resonance-based functional assay for nicotinamide adenine dinucleotide synthetase.
Nicotinamide adenine dinucleotide synthetase (NadE) is an essential enzyme for bacterial pathogens and is thus a promising antibacterial target. It catalyzes the conversion of nicotinic acid adenine dinucleotide to nicotinamide adenine dinucleotide. Changes in chemical shifts that occur in the nicotinic acid ring as it is converted to nicotinamide can be used for monitoring the reaction. A robust nuclear magnetic resonance-based activity assay was developed using robotically controlled reaction initiation and quenching. The single-enzyme assay has less potential for false positives compared to a coupled activity assay and is especially well suited to the high concentration of compounds in fragment screens. The assay has been used to screen fragment libraries for NadE inhibitors.
Stockman
Brian J
BJ
Pfizer Global Research and Development, Groton, Connecticut 06340, USA. brian.j.stockman@pfizer.com
Lodovice
Ian J
IJ
Fisher
Douglas A
DA
McColl
Alexander S
AS
Xie
Zhi
Z
D019732:Amide Synthases / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D000595:Amino Acid Sequence; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009525:Niacin / Q000737:chemistry / Q000378:metabolism; D009536:Niacinamide / Q000096:biosynthesis / Q000737:chemistry; D013211:Staphylococcus aureus / Q000201:enzymology / Q000235:genetics
D009536:Niacinamide; D009525:Niacin; D019732:Amide Synthases; C038479:NAD+ synthase