Optimization of carbon and nitrogen sources and growth factors for the production of an aquaculture probiotic (Pseudomonas MCCB 103) using response surface methodology.
OBJECTIVE
To develop a new medium for enhanced production of biomass of an aquaculture probiotic Pseudomonas MCCB 103 and its antagonistic phenazine compound, pyocyanin.


RESULTS
Carbon and nitrogen sources and growth factors, such as amino acids and vitamins, were screened initially in a mineral medium for the biomass and antagonistic compound of Pseudomonas MCCB 103. The selected ingredients were further optimized using a full-factorial central composite design of the response surface methodology. The medium optimized as per the model for biomass contained mannitol (20 g l(-1)), glycerol (20 g l(-1)), sodium chloride (5 g l(-1)), urea (3.3 g l(-1)) and mineral salts solution (20 ml l(-1)), and the one optimized for the antagonistic compound contained mannitol (2 g l(-1)), glycerol (20 g l(-1)), sodium chloride (5.1 g l(-1)), urea (3.6 g l(-1)) and mineral salts solution (20 ml l(-1)). Subsequently, the model was validated experimentally with a biomass increase by 19% and fivefold increase of the antagonistic compound.


CONCLUSIONS
Significant increase in the biomass and antagonistic compound production could be obtained in the new media.


CONCLUSIONS
Media formulation and optimization are the primary steps involved in bioprocess technology, an attempt not made so far in the production of aquaculture probiotics.
Preetha
R
R
National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Cochin, India.
Jayaprakash
N S
NS
Philip
R
R
Bright Singh
I S
IS
D017756:Aquaculture / Q000379:methods; D018533:Biomass; D001709:Biotechnology / Q000379:methods; D002244:Carbon / Q000378:metabolism*; D003470:Culture Media / Q000032:analysis / Q000737:chemistry*; D008954:Models, Biological; D015233:Models, Statistical; D009584:Nitrogen / Q000378:metabolism*; D019936:Probiotics / Q000378:metabolism*; D011549:Pseudomonas / Q000254:growth & development / Q000378:metabolism*
D003470:Culture Media; D002244:Carbon; D009584:Nitrogen