Hepatic stellate cell damage may lead to decreased plasma ADAMTS13 activity in rats.
ADAMTS13 is gaining attention, because its deficiency causes thrombotic thrombocytopenic purpura. Although its regulatory mechanism is not fully understood, we wondered if hepatic stellate cells (HSCs) play a role, because ADAMTS13 mRNA is exclusively expressed in the liver and primarily in HSCs. Plasma ADAMTS13 activity was markedly reduced in dimethylnitrosamine-treated rats, where HSC apoptosis is an essential event, but not in carbon tetrachloride- or thioacetamide-treated rats without HSC apoptosis. Furthermore, plasma ADAMTS13 activity was also reduced in 70% hepatectomized rats, where HSC loss occurs. These results suggest that HSC may be involved in the regulation of plasma ADAMTS13 activity.
Kume
Yukio
Y
Department of Laboratory Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
Ikeda
Hitoshi
H
Inoue
Morihiro
M
Tejima
Kazuaki
K
Tomiya
Tomoaki
T
Nishikawa
Takako
T
Watanabe
Naoko
N
Ichikawa
Tatsuya
T
Kaneko
Makoto
M
Okubo
Shigeo
S
Yokota
Hiromitsu
H
Omata
Masao
M
Fujiwara
Kenji
K
Yatomi
Yutaka
Y
D051722:ADAM Proteins / Q000097:blood*; D000071120:ADAMTS13 Protein; D000818:Animals; D017209:Apoptosis; D004128:Dimethylnitrosamine / Q000633:toxicity; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D051722:ADAM Proteins; D000071120:ADAMTS13 Protein; C512067:Adamts13 protein, rat; D004128:Dimethylnitrosamine