The effect of ionizing irradiation on the lipid biosynthesis by thymus and liver.
New Zealand rabbits, fasted for 12 hours, were subjected to 500 rads of whole-body irradiation. Analysis of thymus lipids, at various time intervals following irradiation, showed a threefold increase of triglycerides at 24 hours. Fatty acid composition of the 600 X g supernatant was not affected at 24 hours after irradiation. Lipid biosynthesis from acetate-1-14C by the thymus homogenates was increased to a small extent at 4 hours following irradiation, while the radioactivity distribution into fatty acids was not considerably affected. Contrary to the above findings, fatty acid synthesis from acetate-l-14C by the liver preparations showed a decreased incorporation between the fourth and twelfth hour following irradiation. Counting of the radioactivity of the separated fatty acids suggested that the system for synthesis of short-chain fatty acids was impaired as early as 4 hours following irradiation.
Miras
C J
CJ
Biochemical Research Laboratory, School of Medicine, Department of Clinical Therapeutics, University of Athens, Alexandra Hospital, Athens, Greece.
Mantzos
J D
JD
Legakis
N J
NJ
Levis
G M
GM
D000818:Animals; D004307:Dose-Response Relationship, Radiation; D005260:Female; D008055:Lipids / Q000096:biosynthesis*; D008099:Liver / Q000378:metabolism* / Q000528:radiation effects*; D008297:Male; D011817:Rabbits; D011829:Radiation Dosage; D011839:Radiation, Ionizing; D013950:Thymus Gland / Q000378:metabolism* / Q000528:radiation effects*; D014916:Whole-Body Irradiation
D008055:Lipids