Analysis of glycans in glycoproteins by diffusion-ordered nuclear magnetic resonance spectroscopy.
Enzymatically cleaved glycans from sub-milligram quantities of erythropoietin (EPO) and ovalbumin have been analyzed, without further purification, by two-dimensional diffusion-ordered nuclear magnetic resonance spectroscopy. At NMR sample concentrations below 50 micromol L-1 the major components of the oligosaccharide fractions could be distinguished by their anomeric proton chemical shift and their size-dependent diffusion coefficients.
Ortner
Karin
K
Institute of Analytical Chemistry, Johannes Kepler University, Altenberger Strasse 69, 4040, Linz, Austria.
Sivanandam
Veeramuthu N
VN
Buchberger
Wolfgang
W
Müller
Norbert
N
D004058:Diffusion; D004921:Erythropoietin / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010047:Ovalbumin / Q000737:chemistry; D011134:Polysaccharides / Q000032:analysis*
D006023:Glycoproteins; D011134:Polysaccharides; D004921:Erythropoietin; D010047:Ovalbumin