title: Apple polyphenols and products formed in the gut differently inhibit survival of human cell lines derived from colon adenoma (LT97) and carcinoma (HT29).
abstract: Colorectal tumor risks could be reduced by polyphenol-rich diets that inhibit cell growth. Here, apple polyphenols were studied for effects on the survival of colon adenoma (LT97) and carcinoma-derived (HT29) cell lines. Three apple extracts (AEs) from harvest years 2002-2004 were isolated (AE02, AE03, and AE04) and fermented in vitro with human fecal flora. Extracts and fermentation products were analyzed for polyphenols with HPLC. The cells were treated with AEs (0-850 microg/mL) or fermented AEs (F-AEs, 0-9%), and survival was measured by DNA staining. All AEs contained high amounts of polyphenols (311-534 mg/g) and reduced cell survival (in LT97 > HT29). AE03 was most potent, possibly because it contained more quercetin compounds. Fermentation of AEs resulted in an increase of short chain fatty acids, and polyphenols were degraded. The F-AEs were approximately 3-fold less bioactive than the corresponding AEs, pointing to a loss of chemoprotective properties through fermentation.
authors: Department of Nutritional Toxicology, Institute for Nutrition, Friedrich-Schiller-University, Dornburger Strasse 25, D-07743 Jena, Germany.
authors: Selvaraju
authors: S
authors: Veeriah
authors: Thomas
authors: T
authors: Hofmann
authors: Michael
authors: M
authors: Glei
authors: Helmut
authors: H
authors: Dietrich
authors: Frank
authors: F
authors: Will
authors: Peter
authors: P
authors: Schreier
authors: Bastian
authors: B
authors: Knaup
authors: Beatrice Louise
authors: BL
authors: Pool-Zobel
mesh_terms: D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000473:pathology* / Q000517:prevention & control; D005285:Fermentation; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology*; D005638:Fruit / Q000737:chemistry*; D019073:HT29 Cells; D006801:Humans; D027845:Malus / Q000737:chemistry*; D010636:Phenols / Q000032:analysis / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D059808:Polyphenols
chemical_list: D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols