Rapid hyperspectral fluorescence lifetime imaging.
We report a rapid hyperspectral fluorescence lifetime imaging (FLIM) instrument that exploits high-speed FLIM technology in a line-scanning microscope. We demonstrate the acquisition of whole-field optically sectioned hyperspectral fluorescence lifetime image stacks (with 32 spectral bins) in less than 40 s and illustrate its application to unstained biological tissue.
De Beule
Pieter
P
Department of Physics, Imperial College London, South Kensington Campus, London, SW7 2AZ, United Kingdom.
Owen
Dylan M
DM
Manning
Hugh B
HB
Talbot
Clifford B
CB
Requejo-Isidro
Jose
J
Dunsby
Christopher
C
McGinty
James
J
Benninger
Richard K P
RK
Elson
Daniel S
DS
Munro
Ian
I
John Lever
M
M
Anand
Praveen
P
Neil
Mark A A
MA
French
Paul M W
PM
D001158:Arteries / Q000737:chemistry / Q000648:ultrastructure; D031405:Convallaria / Q000737:chemistry / Q000648:ultrastructure; D003952:Diagnostic Imaging / Q000379:methods*; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D013997:Time Factors