title: Genomic structure and transcriptional regulation of the rat, mouse, and human carboxylesterase genes.
abstract: The mammalian carboxylesterases (CESs) comprise a multigene family which gene products play important roles in biotransformation of ester- or amide-type prodrugs. Since expression level of CESs may affect the pharmacokinetic behavior of prodrugs in vivo, it is important to understand the transcriptional regulation mechanism of the CES genes. However, little is known about the gene structure and transcriptional regulation of the mammalian CES genes. In the present study, to investigate the transcriptional regulation of the promoter region of the CES1 and CES2 genes were isolated from mouse, rat and human genomic DNA by PCR amplification. A TATA box was not found the transcriptional start site of all CES promoter. These CES promoters share several common binding sites for transcription factors among the same CES families, suggesting that the orthologous CES genes have evolutionally conserved transcriptional regulatory mechanisms. The result of present study suggested that the mammalian CES promoters were at least partly conserved among the same CES families, and some of the transcription factors may play similar roles in transcriptional regulation of the human and murine CES genes.
authors: Faculty of Pharmaceutical Sciences, Chiba Institute of Sciences, Choshi, 288-0025, Japan. masakiyo@cis.ac.jp
authors: Masakiyo
authors: M
authors: Hosokawa
authors: Tomomi
authors: T
authors: Furihata
authors: Yumiko
authors: Y
authors: Yaginuma
authors: Naoko
authors: N
authors: Yamamoto
authors: Nao
authors: N
authors: Koyano
authors: Ayako
authors: A
authors: Fujii
authors: Yuko
authors: Y
authors: Nagahara
authors: Testuo
authors: T
authors: Satoh
authors: Kan
authors: K
authors: Chiba
mesh_terms: D000818:Animals; D043182:Carboxylesterase / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D051379:Mice; D010802:Phylogeny; D011401:Promoter Regions, Genetic; D051381:Rats; D013379:Substrate Specificity; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: D043182:Carboxylesterase