Benzo[a]pyrene bioavailability from pristine soil and contaminated sediment assessed using two in vitro models.
A major route of exposure to hydrophobic organic contaminants (HOCs), such as benzo[a]pyrene (BaP), is ingestion. Matrix-bound HOCs may become bioavailable after mobilization by the gastrointestinal fluids followed by sorption to the intestinal epithelium. The purpose of this research was to measure the bioavailability of [14C]-BaP bound to pristine soils or field-contaminated sediment using an in vitro model of gastrointestinal digestion followed by sorption to human enterocytes (Caco-2 cells) or to a surrogate membrane, ethylene vinyl acetate (EVA) thin film. Although Caco-2 cells had a twofold higher lipid-normalized fugacity capacity than EVA, [14C]-BaP uptake by Caco-2 lipids and EVA thin film demonstrated a linear relationship within the range of BaP concentrations tested. These results suggest that EVA thin film is a good membrane surrogate for passive uptake of BaP. The in vitro system provided enough sensitivity to detect matrix effects on bioavailability; after 5 h, significantly lower concentrations of [14C]-BaP were sorbed into Caco-2 cells from soil containing a higher percentage of organic matter compared to soil with a lower percentage of organic matter. The [14C]-BaP desorption rate from Caco-2 lipids consistently was twofold higher than from EVA thin film for all matrices tested. The more rapid kinetics observed with Caco-2 cells probably were due to the greater surface area available for absorption/desorption in the cells. After 5 h, the uptake of BaP into Caco-2 lipid was similar in live and metabolically inert Caco-2 cells, suggesting that the primary route of BaP uptake is by passive diffusion. Moreover, the driving force for uptake is the fugacity gradient that exists between the gastrointestinal fluid and the membrane.
Vasiluk
Luba
L
Department of Biological Sciences, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada.
Pinto
Linda J
LJ
Walji
Zahra A
ZA
Tsang
Wing Shan
WS
Gobas
Frank A P C
FA
Eickhoff
Curtis
C
Moore
Margo M
MM
D001564:Benzo(a)pyrene / Q000493:pharmacokinetics*; D001682:Biological Availability; D002460:Cell Line; D002463:Cell Membrane Permeability; D004058:Diffusion; D020895:Enterocytes / Q000166:cytology / Q000378:metabolism*; D019015:Geologic Sediments; D006801:Humans; D007700:Kinetics; D008954:Models, Biological; D011145:Polyvinyls; D012987:Soil; D012989:Soil Pollutants / Q000493:pharmacokinetics*
D011145:Polyvinyls; D012987:Soil; D012989:Soil Pollutants; C016438:ethylenevinylacetate copolymer; D001564:Benzo(a)pyrene