[Effect of interleukin-1alpha on the phagocytic function in cultured human trabecular meshwork cells].
OBJECTIVE
To investigate the effect of interleukin-1alpha (IL-1alpha) on the phagocytic function in cultured human trabecular meshwork (HTM) cells.


METHODS
HTM Cells were cultured and subcultured in vitro. The third to fifth passage of HTM cells were treated with different concentrations of IL-1alpha or vehicle in DMEM/F12 medium. 1000 of 0.85 microm microsphere/per cell were added to each well. The morphological changes of HTM cells were observed by an inverted phase-contrast microscope and electron microscope. The cellular phagocytic function of HTM cells was evaluated by counting the numbers of microsphere in 20 HTM cells using a light microscope.


RESULTS
The break of HTM cell membrane was found under the electron microscope. The numbers of microsphere in HTM cells was significantly (P = 0.005) reduced in a dose- and incubation time-dependent manner (F = 3.603, P = 0.046, F = 15.846, P = 0.005, respectively).


CONCLUSIONS
IL-1alpha affects the phagocytic function of HTM cells that is correlated to the concentration and the incubation time.
Li
Xin-yu
XY
Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. lixy07@126.com
Wang
Chao-qiong
CQ
Xiang
Nan
N
Zhou
Xiong-wu
XW
D002478:Cells, Cultured; D006801:Humans; D053582:Interleukin-1alpha / Q000494:pharmacology*; D010587:Phagocytosis; D014129:Trabecular Meshwork / Q000166:cytology / Q000187:drug effects* / Q000502:physiology* / Q000648:ultrastructure
D053582:Interleukin-1alpha