Automated measurement of permeation and dissolution of propranolol HCl tablets using sequential injection analysis.
Aim of this study was to automate sampling and quantification of the previously described apparatus for combined determination of dissolution and permeation through Caco-2 monolayer by means of sequential injection analysis (SIA). Native fluorescence of propranolol HCl in Krebs-Ringer buffer (KRB) was used for quantification. Sampling was done at three different locations within the apparatus at a high sampling frequency (approximately 60 h(-1)). Injection volume delivered to the fluorescence detector was 50 microL for permeation monitoring and 25 microL for dissolution monitoring. Linear regression for 50 microL injection yielded a detection limit calculated as 0.04 microg mL(-1) of propranolol HCl in KRB (R(2)>0.999). However, linearity for dissolution monitoring was not given for the complete range of concentrations and first order polynomial calibration was established (R(2)>0.9999). To conclude, the SIA system was able to monitor simultaneously dissolution and permeation of the immediate release propranolol HCl tablets and the authors succeeded in automating the apparatus for combined measurement of dissolution and permeation. In addition, the obtained data was consistent with data obtained by manual sampling followed by HPLC analysis.
Motz
Stephan A
SA
Saarland University, Biopharmaceutics and Pharmaceutical Technology, Im Stadtwald, Geb. A4.1, P.O. Box 15 11 50, 66041 Saarbruecken, Germany.
Klimundová
Jana
J
Schaefer
Ulrich F
UF
Balbach
Stefan
S
Eichinger
Thomas
T
Solich
Petr
P
Lehr
Claus-Michael
CM
D018938:Caco-2 Cells; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods; D006801:Humans; D010539:Permeability / Q000187:drug effects; D011433:Propranolol / Q000032:analysis* / Q000493:pharmacokinetics*; D012107:Research Design; D012995:Solubility / Q000187:drug effects; D013607:Tablets
D013607:Tablets; D011433:Propranolol