title: The importance of blood sampling site for determination of hemoglobin and biochemistry values in major abdominal and orthopedic surgery.
abstract: OBJECTIVE
  To determine whether sampling of blood from different sites influences laboratory results.
  
  
  METHODS
  Prospective, double-blind study.
  
  
  METHODS
  University-affiliated hospital in Israel.
  
  
  METHODS
  100 ASA physical status I, II, and III patients undergoing major orthopedic or colon surgery (total hip and revision of total hip replacement, colon resection, or radical cystectomy).
  
  
  METHODS
  Blood was sampled simultaneously for hemoglobin, electrolytes, glucose, pH, blood gases, and lactate from three sampling sites (peripheral vein, central vein, and radial artery) at 5 time frames (after induction of anesthesia [baseline], one hr after induction of anesthesia, at the end of surgery, after one hr in the recovery room, and 4 hrs after surgery). At the same time points, recorded rectal temperature, mean arterial pressure, heart rate, and central venous pressure were recorded. Anesthesia, monitoring, and dwell volumes before sampling were standardized.
  
  
  RESULTS
  There were no significant differences between the results of hemoglobin, electrolytes, glucose, pH, and blood gases obtained from different sampling sites and at different time frames. Lactate level (mmol/L) was higher in peripheral venous blood than it was in either the central vein or radial artery (<0.05), and higher in central venous blood compared with arterial blood (P < 0.05; 2.04 +/- 1.16, 1.74 +/- 0.78, and 1.54 +/- 0.68, respectively).
  
  
  CONCLUSIONS
  Under stable hemodynamics and in the absence of hypothermia, serum lactate level was higher in peripheral venous blood than it was in the central vein or radial artery.
authors: Department of Anesthesia, Edith Wolfson Medical Center, Holon 58100, Israel.
authors: Shmuel
authors: S
authors: Evron
authors: Vladimir
authors: V
authors: Tress
authors: Tiberiu
authors: T
authors: Ezri
authors: Peter
authors: P
authors: Szmuk
authors: Ofer
authors: O
authors: Landau
authors: David
authors: D
authors: Hendel
authors: Pinhas
authors: P
authors: Schechter
authors: Benjamin
authors: B
authors: Medalion
mesh_terms: D000368:Aged; D001158:Arteries; D019644:Arthroplasty, Replacement, Hip / Q000379:methods*; D001784:Blood Gas Analysis / Q000379:methods; D001786:Blood Glucose / Q000032:analysis*; D001794:Blood Pressure; D001800:Blood Specimen Collection / Q000379:methods*; D003106:Colon / Q000601:surgery*; D004311:Double-Blind Method; D004573:Electrolytes / Q000097:blood; D005260:Female; D006339:Heart Rate; D006454:Hemoglobins / Q000032:analysis*; D006785:Hospitals, University; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007557:Israel; D019344:Lactic Acid / Q000097:blood*; D008297:Male; D011446:Prospective Studies; D013997:Time Factors; D014680:Veins
chemical_list: D001786:Blood Glucose; D004573:Electrolytes; D006454:Hemoglobins; D019344:Lactic Acid