Determination of paroxetine in human saliva by reversed-phase high-performance liquid chromatography with UV detection.
A rapid and sensitive high-performance liquid chromatographic method was validated and described for determination of paroxetine in human saliva. Following liquid-liquid extraction of the drug and an internal standard (dibucaine), chromatographic separation was accomplished using a C18 analytical column with a mobile phase consisting of 0.05 mol/L sodium phosphate buffer, pH 5.0, and acetonitrile (A 30:70, v/v; B 60:40, v/v). Paroxetine and the internal standard were detected by ultraviolet absorbance at 205 nm. The average recoveries of the drug and internal standard were 92.5% and 89%, respectively. The lower limits of detection and quantification were 1 and 4 ng/ml, respectively, and the calibration curve was linear over a concentration range of 4 ng/ml. The saliva level of paroxetine in patients with depression taking 10 to 40 mg/day of the drug was significantly correlated with the plasma level of paroxetine in each patient (r = 0.617, P < 0.004, n = 19). These data indicate that the saliva level of paroxetine could be a useful marker to predict the plasma level of the drug.
Tsuruta
Toshinori
T
Division of Pharmacy, Saga University Medical Hospital. tsuruta@cc.saga-u.ac.jp
Yang
Chunyan
C
Ueki
Hiroshi
H
Li
Guangying
G
Maekawa
Akiko
A
Kamikawa
Hideki
H
Oku
Eisaku
E
Somehara
Takao
T
Fujito
Hiroshi
H
Tatebayashi
Hideharu
H
Yamada
Shigeto
S
D018687:Antidepressive Agents, Second-Generation / Q000032:analysis* / Q000097:blood; D015415:Biomarkers / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003863:Depression / Q000188:drug therapy / Q000378:metabolism; D016903:Drug Monitoring / Q000379:methods*; D006801:Humans; D017374:Paroxetine / Q000032:analysis* / Q000097:blood; D010349:Patient Compliance; D012463:Saliva / Q000737:chemistry*
D018687:Antidepressive Agents, Second-Generation; D015415:Biomarkers; D017374:Paroxetine