Mass spectrometric detection of and similarities between 1-androgens.
Regularly new anabolic steroids appear on the black market. In most cases these substances are marketed on websites or are confiscated during inspections. 1,(5alpha)-Androstene-17beta-ol-3-one, also known as 1-testosterone, is one of these substances presented to body-builders as a nutritional supplement or a pro-hormone. 1-Testosterone closely resembles the natural hormone testosterone except for a 1,2-double bound instead of a 4,5-double bound. 1-Androstene-3beta,17beta-diol is transformed into 1-testosterone after oral administration. 1-Testosterone, 1-androstene-3beta,17beta-diol and some other related 'new' anabolic steroids were studied with gas chromatography coupled to mass spectrometry (GC-MS) and Liquid chromatography coupled to tandem mass spectrometry (LC-MS2) methods. Similarities in spectra to known analytes, which may lead to pitfalls in the interpretation of the derivatised analytes, are discussed.
Verheyden
K
K
Ghent University, Faculty of Veterinary Medicine, Department of Veterinary Public Health and Food Safety, Laboratory of Chemical Analysis, Salisburylaan 133, B-9820 Merelbeke, Belgium.
Le Bizec
B
B
Courtheyn
D
D
Mortier
V
V
Vandewiele
M
M
Gillis
W
W
Vanthemsche
P
P
De Brabander
H F
HF
Noppe
H
H
D000284:Administration, Oral; D045930:Anabolic Agents / Q000032:analysis*; D000728:Androgens / Q000032:analysis*; D015114:Androstenediol / Q000032:analysis; D002849:Chromatography, Gas / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods*; D004300:Doping in Sports; D008401:Gas Chromatography-Mass Spectrometry; D013058:Mass Spectrometry / Q000379:methods*; D008956:Models, Chemical; D013256:Steroids / Q000032:analysis*; D015813:Substance Abuse Detection / Q000379:methods; D013739:Testosterone / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry; D014891:Weight Lifting
D045930:Anabolic Agents; D000728:Androgens; D013256:Steroids; D013739:Testosterone; D015114:Androstenediol; C520094:1-testosterone