title: Cloning and spatial and temporal expression of the zebrafish dopamine D1 receptor.
abstract: Dopamine plays important roles in the regulation of central nervous system (CNS) development and functions. In vertebrates, two families of dopamine receptors, collectively known as dopamine D1 and D2 receptors, have been identified. Recently, dopamine receptors have been targeted by pharmacological and therapeutic studies of neurological disorders, such as Parkinson's disease. Here, we report a study on the molecular characterization of dopamine D1 receptor in zebrafish (Danio rerio). We cloned the full-length cDNA of a zebrafish dopamine D1 receptor, designated as drd1. The sequence of drd1 shares high homology to the sequences of dopamine D1 receptors in mammalian, amphibian, and other fish species. drd1 is expressed in the CNS. The first drd1 expression was observed at approximately 30 hours postfertilization, at which time the expression was seen in the developing diencephalon and hindbrain. In developing retinas, the expression of drd1 was detected in the inner nuclear layer with the exception of the marginal zones. In adult retinas, drd1 expression was detected in most cell types in the inner and outer nuclear layers as well as ganglion cell layer. Differential expression of drd1 in developing and adult retinas may play various roles in regulating visual system functions.
authors: Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana 46556, USA.
authors: Ping
authors: P
authors: Li
authors: Sitar
authors: S
authors: Shah
authors: Luoxiu
authors: L
authors: Huang
authors: Aprell L
authors: AL
authors: Carr
authors: Yan
authors: Y
authors: Gao
authors: Christine
authors: C
authors: Thisse
authors: Bernard
authors: B
authors: Thisse
authors: Lei
authors: L
authors: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002490:Central Nervous System / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D010802:Phylogeny; D017447:Receptors, Dopamine D1 / Q000235:genetics*; D017448:Receptors, Dopamine D2 / Q000235:genetics; D012160:Retina / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015027:Zebrafish / Q000196:embryology / Q000235:genetics* / Q000254:growth & development; D029961:Zebrafish Proteins / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2; D029961:Zebrafish Proteins