title: In vitro and in vivo studies of the Interferon-alpha action on distinct Orthobunyavirus.
abstract: Oropouche, Caraparu, Guama, Guaroa and Tacaiuma viruses (Orthobunyavirus genus) cause human febrile illnesses and/or encephalitis. To achieve a therapeutical agent to prevent and/or treat these diseases we evaluated the antiviral action of Interferon-alpha (IFN-alpha) on these orthobunyaviruses. In vitro results showed that all the studied orthobunyaviruses are susceptible to antiviral action of IFN-alpha, but this susceptibility is limited and dependent on both concentration of drug and treatment period. In vivo results demonstrated that IFN-alpha present antiviral action on Oropouche and Guaroa viruses when used as a prophylactic treatment. Moreover, a treatment initiated 3h after infection prevented the death of Guaroa virus infected-mice. Additionally, mortality of mice was related to the migration and replication of viruses in their brains. Our results suggest that IFN-alpha could be potentially useful in the prevention of diseases caused by Oropouche virus and in the prevention and/or treatment of diseases caused by Guaroa virus.
authors: Center for Research in Virology, School of Medicine of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil. pink@rpm.fmrp.usp.br
authors: Márcia Cristina
authors: MC
authors: Livonesi
authors: Ricardo Luiz Moro
authors: RL
authors: de Sousa
authors: Soraya Jabur
authors: SJ
authors: Badra
authors: Luiz Tadeu Moraes
authors: LT
authors: Figueiredo
mesh_terms: D000818:Animals; D000833:Animals, Suckling; D000998:Antiviral Agents / Q000494:pharmacology / Q000627:therapeutic use; D001835:Body Weight / Q000187:drug effects; D001921:Brain / Q000187:drug effects / Q000821:virology; D002044:Bunyaviridae Infections / Q000188:drug therapy* / Q000401:mortality / Q000517:prevention & control; D002522:Chlorocebus aethiops; D004305:Dose-Response Relationship, Drug; D007370:Interferon Type I / Q000494:pharmacology / Q000627:therapeutic use; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000494:pharmacology / Q000627:therapeutic use*; D051379:Mice; D016851:Orthobunyavirus / Q000187:drug effects* / Q000254:growth & development; D011994:Recombinant Proteins; D015996:Survival Rate; D013997:Time Factors; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D007370:Interferon Type I; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins