NMR structure determination of protein-ligand complexes by lanthanide labeling.
The paramagnetism of lanthanide ions offers outstanding opportunities for fast determinations of the three-dimensional (3D) structures of protein-ligand complexes by nuclear magnetic resonance (NMR) spectroscopy. It is shown how the combination of pseudocontact shifts (PCSs) induced by a site-specifically bound lanthanide ion and prior knowledge of the 3D structure of the lanthanide-labeled protein can be used to achieve (i) rapid assignments of NMR spectra, (ii) structure determinations of protein-protein complexes, and (iii) identification of the binding mode of low-molecular weight compounds in complexes with proteins. Strategies for site-specific incorporation of lanthanide ions into proteins are summarized.
Pintacuda
Guido
G
Research School of Chemistry, Australian National University, Canberra, ACT 0200, Australia.
John
Michael
M
Su
Xun-Cheng
XC
Otting
Gottfried
G
D028581:Lanthanoid Series Elements / Q000737:chemistry*; D008024:Ligands; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*
D028581:Lanthanoid Series Elements; D008024:Ligands; D011506:Proteins