Regression analysis and modelling of data acquisition for SELDI-TOF mass spectrometry.
BACKGROUND
Pre-processing of SELDI-TOF mass spectrometry data is currently performed on a largel y ad hoc basis. This makes comparison of results from independent analyses troublesome and does not provide a framework for distinguishing different sources of variation in data.


RESULTS
In this article, we consider the task of pooling a large number of single-shot spectra, a task commonly performed automatically by the instrument software. By viewing the underlying statistical problem as one of heteroscedastic linear regression, we provide a framework for introducing robust methods and for dealing with missing data resulting from a limited span of recordable intensity values provided by the instrument. Our framework provides an interpretation of currently used methods as a maximum-likelihood estimator and allows theoretical derivation of its variance. We observe that this variance depends crucially on the total number of ionic species, which can vary considerably between different pooled spectra. This variation in variance can potentially invalidate the results from naive methods of discrimination/classification and we outline appropriate data transformations. Introducing methods from robust statistics did not improve the standard errors of the pooled samples. Imputing missing values however-using the EM algorithm-had a notable effect on the result; for our data, the pooled height of peaks which were frequently truncated increased by up to 30%.
Sköld
Martin
M
Centre for Mathematical Sciences, Lund University, Lund, Sweden. martins@maths.lth.se
Rydén
Tobias
T
Samuelsson
Viktoria
V
Bratt
Charlotte
C
Ekblad
Lars
L
Olsson
Håkan
H
Baldetorp
Bo
B
D000465:Algorithms; D003198:Computer Simulation; D003627:Data Interpretation, Statistical; D046228:Microarray Analysis / Q000379:methods*; D015233:Models, Statistical; D012044:Regression Analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*