Determination of acrylamide in food by solid-phase microextraction coupled to gas chromatography-positive chemical ionization tandem mass spectrometry.
A method has been developed to determine acrylamide in aqueous matrices by using direct immersion solid-phase microextraction (SPME) coupled to gas chromatography-positive chemical ionization tandem mass spectrometry (GC-PCI-MS-MS) in the selected reaction monitoring (SRM) mode. The optimized SPME experimental procedures to extract acrylamide in water solutions were: use of a carbowax/divinylbenzene (CW/DVB)-coated fiber at pH 7, extraction time of 20 min and analyte desorption at 210 degrees C for 3 min. A detection limit of 0.1 microg L(-1) was obtained. The linear range was 1-1000 microg L(-1). The relative standard deviation was 10.64% (n=7). The proposed analytical method was successfully used for the quantification of trace acrylamide in foodstuffs such as French fries (1.2 microg g(-1)) and potato crisps (2.2 microg g(-1)).
Lee
Maw-Rong
MR
Department of Chemistry, National Chung Hsing University, Taichung 40227, Taiwan, ROC. mrlee@dragon.nchu.edu.tw <mrlee@dragon.nchu.edu.tw>
Chang
Li-Yo
LY
Dou
Jianpeng
J
D020106:Acrylamide / Q000032:analysis*; D005504:Food Analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D011198:Solanum tuberosum / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D020106:Acrylamide