Surveillance for reassortant virus by multiplex reverse transcription-PCR specific for eight genomic segments of avian influenza A H5N1 viruses.
Avian influenza H5N1 virus is a global threat. An emergence of a reassortant virus with a pandemic potential is a major concern. Here we describe a multiplex reverse transcription-PCR assay that is specific for the eight genomic segments of the currently circulating H5N1 viruses to facilitate surveillance for a virus resulting from reassortment between human influenza virus and the H5N1 virus.
Auewarakul
Prasert
P
Department of Microbiology, Faculty of Medicine, Siriraj Hospital, Mahidol University, 2 Prannok Road, Bangkok Noi, Bangkok 10700, Thailand.
Sangsiriwut
Kantima
K
Chaichoune
Kridsda
K
Thitithanyanont
Arunee
A
Wiriyarat
Witthawat
W
Songserm
Taweesak
T
Ponak-nguen
Rasameepen
R
Prasertsopon
Jarunee
J
Pooruk
Phisanu
P
Sawanpanyalert
Pathom
P
Ratanakorn
Parntep
P
Puthavathana
Pilaipan
P
D016679:Genome, Viral; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics* / Q000302:isolation & purification*; D007251:Influenza, Human / Q000453:epidemiology / Q000821:virology; D011159:Population Surveillance / Q000379:methods; D016865:Reassortant Viruses / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*