Cooperation of group 2 sigma factors, SigD and SigE for light-induced transcription in the cyanobacterium Synechocystis sp. PCC 6803.
A light-inducible sigma factor of RNA polymerase, SigD, can contributes to the light-induced transcription of psbA in the cyanobacterium Synechocystis sp. PCC 6803. Here, another light-induced sigma factor, SigE, was characterized together with SigD. Results indicated that SigE also contributes to light-induced transcription on the cpcBACD, psbA, petBD and psaAB promoters whose potential sequences are of the Escherichia coli sigma(70)-type. SigD and SigE interfere with each other's expression. A rhythmic expression, in which the periodic peak of SigE exhibits a 24-h interval according to the upcoming night, was observed at the protein level. The cooperation of group 2 sigma factors, SigD and SigE, for light-induced transcription was discussed.
Yoshimura
Tsutomu
T
Laboratory of Molecular Genetics, School of Agriculture, Ibaraki University, 3-21-1 Ami, Inashiki, Ibaraki 300-0393, Japan.
Imamura
Sousuke
S
Tanaka
Kan
K
Shirai
Makoto
M
Asayama
Munehiko
M
D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000235:genetics; D008027:Light; D011401:Promoter Regions, Genetic / Q000528:radiation effects; D012808:Sigma Factor / Q000378:metabolism*; D046939:Synechocystis / Q000235:genetics* / Q000528:radiation effects; D014158:Transcription, Genetic / Q000528:radiation effects*
D012808:Sigma Factor