Time-resolved fluoroimmunoassay for 19-nortestosterone residues in aquaculture tissues.
A competitive time-resolved fluoroimmunoassay (TR-FIA) was developed for the determination of 19-nortestosterone (17beta-NT) residues in aquaculture tissues. The limit of detection (LOD) was determined to be 0.08 ng g-1 and the limit of quantification (LOQ) was less than 0.8 ng g-1. The results obtained by the TR-FIA and ELISA showed a good correlation. The established TR-FIA was validated for the determination of incurred aquaculture tissues and confirmed by liquid chromatography tandem mass spectrometry (LC/MS/MS). This proposed technique could be applied to routine residue analysis.
Li
Xiangqian
X
Department of Chemical and Bioengineering, Huaiyin Institute of Technology, Huai'an, JiangSu Province, China.
Huo
Tieming
T
Chu
Xiaogang
X
Xu
Chuanlai
C
D000818:Animals; D017756:Aquaculture; D004350:Drug Residues / Q000032:analysis*; D005399:Fishes; D015200:Fluoroimmunoassay / Q000379:methods*; D008460:Meat / Q000032:analysis*; D009277:Nandrolone / Q000032:analysis* / Q000276:immunology*; D015203:Reproducibility of Results
D009277:Nandrolone