title: Activation of mononuclear bone marrow cells treated in vitro with a complex homeopathic medication.
abstract: Canova is a Brazilian homeopathic medication with immunomodulatory properties, recommended for patients where the immune system is depressed. Previous studies demonstrated that Canova induces up-regulation in numbers of leukocytes. The bone marrow microenvironment is composed of growth factors, stromal cells, extracellular matrix and progenitor cells that differentiate into mature blood cells. We now report the effect of in vitro administration of the medication on the mononuclear differentiation of the bone marrow cell. Swiss mice femurs were dissected cleaned and the cells of the marrow were flushed. The cells were plated, treated or not, incubated for different times and processed for light, transmission and scanning electron, and confocal microscopy analysis. Bone marrow cells showed an enhanced proliferation in vitro in response to Canova medication and Canova plus M-CSF and an increase was also observed in the numbers of the cell niches and ring-shaped nuclei cells. Confocal and transmission and scanning electron microscopy showed the stages of monocyte maturation, with resting and activated cells. With Canova treatment there was a marked increase in cell size, which is mainly attributable to the augmented cytoplasm, an increase in the number of mitochondria, expansion of the RER and an enlarged Golgi. The response to Canova treatment indicates that it influences mononuclear differentiation and activation of bone marrow progenitor and stromal cells.
authors: Departamento de Biologia Celular, Setor de Ciências Biológicas, Universidade Federal do Paraná (UFPR), Curitiba, PR, Brazil.
authors: Beatriz
authors: B
authors: Cesar
authors: Ana Paula R
authors: AP
authors: Abud
authors: Carolina C
authors: CC
authors: de Oliveira
authors: Francolino
authors: F
authors: Cardoso
authors: Waldemiro
authors: W
authors: Gremski
authors: Juarez
authors: J
authors: Gabardo
authors: Dorly de Freitas
authors: Dde F
authors: Buchi
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000648:ultrastructure; D003435:Crotalid Venoms / Q000494:pharmacology*; D005571:Formularies, Homeopathic as Topic; D008214:Lymphocytes / Q000187:drug effects / Q000648:ultrastructure; D008262:Macrophage Activation; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: C513575:Canova medication; D003435:Crotalid Venoms; D010936:Plant Extracts; D016173:Macrophage Colony-Stimulating Factor