[Preparation and identification of monoclonal antibody against sporozoites of Eimeria acervulina].
OBJECTIVE
To establish hybridoma cell lines against sporozoites of Eimeria acervulina.


METHODS
BALB/c mice were immunized with purified sporozoites of E. acervulina. Hybridoma cell lines were set up by using hybridoma technique, and monoclonal antibodies were prepared. The monoclonal antibody was identified by determining their cross reactivity, relative affinity, immunoglobulin class or subclass with enzyme linked immunosorbent assay (ELISA).


RESULTS
Four hybridoma cell lines stably secreting McAbs against sporozoites were obtained: Easp-3G3 and Easp-5G10 belonging to IgG1, Easp-3H6 belonging to IgG2b, Easp-5H4 belonging to IgG2a. All four McAbs bound with E. acervulina sporozoite protein, but the Easp-5H4 McAb showed cross reactivity with E. tenellum sporozoite protein. Different antigenic epitopes were recognized by Easp-3G3 or Easp-5G10 and Easp-3H6 or Easp-5H4.


CONCLUSIONS
Three of the four produced monoclonal antibodies show high specificity and affinity to the Eimeria acervulina sporozoites.
Wang
Cheng-Min
CM
Cell Engineering Key Laboratory of Henan Institute of Science and Technology, Xinxiang 453003, China. wangchengmin955@sohu.com
He
Hong-Xuan
HX
Qin
Jian-Hua
JH
Chen
Gui-Xiang
GX
Guo
Qi-Zhen
QZ
Hang
Bo-Lin
BL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification*; D003048:Coccidiosis / Q000469:parasitology*; D004539:Eimeria / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D034101:Sporozoites / Q000276:immunology*
D000911:Antibodies, Monoclonal