title: Adherence to HeLa cells, typing by killer toxins and susceptibility to antifungal agents of Candida dubliniensis strains.
abstract: The aim of this study was to evaluate the adherence capability to HeLa cells, the susceptibility to killer toxins and the in vitro susceptibility to antifungal agents (eTest method--AB Biodisk, Solna, Sweden) of 9 Candida dubliniensis isolates recovered from HIV+ and AIDS patients. The adherence test was strongly positive for strain ATCC 777 and positive for all other strains. Typing by killer toxins revealed two different biotypes among the 9 isolates studied: 888 and 688. Only biotype 688 (ATCC 777) was susceptible to the K2 toxin. There was a significant inverse correlation between adherence and killer toxin susceptibility (r = -0.8525 - p = 0.0035). No strains presented resistance to fluconazole, itraconazole, ketoconazole, voriconazole, flucytosine or amphotericin-B. With the exception of ATCC 777, all the other isolates presented similar behavior.
authors: Department of Stomatology, School of Dentistry, University of São Paulo. gismari.miranda@uol.com.br
authors: Gismari Miranda da
authors: GM
authors: Silva
authors: Fernando Ricardo Xavier da
authors: FR
authors: Silveira
authors: Maria de Fátima Costa
authors: Mde F
authors: Pires
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology; D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000145:classification / Q000187:drug effects* / Q000502:physiology; D002448:Cell Adhesion; D004352:Drug Resistance, Microbial; D005656:Fungal Proteins / Q000032:analysis*; D015658:HIV Infections / Q000382:microbiology; D006367:HeLa Cells / Q000382:microbiology; D006801:Humans; D055646:Killer Factors, Yeast; D008826:Microbial Sensitivity Tests; D016533:Mycological Typing Techniques; D011506:Proteins / Q000032:analysis / Q000494:pharmacology
chemical_list: D000935:Antifungal Agents; D005656:Fungal Proteins; D055646:Killer Factors, Yeast; D011506:Proteins