Single feature polymorphism discovery in rice.
The discovery of nucleotide diversity captured as single feature polymorphism (SFP) by using the expression array is a high-throughput and effective method in detecting genome-wide polymorphism. The efficacy of such method was tested in rice, and the results presented in the paper indicate high sensitivity in predicting SFP. The sensitivity of polymorphism detection was further demonstrated by the fact that no biasness was observed in detecting SFP with either single or multiple nucleotide polymorphisms. The high density SFP data that can be generated quite effectively by the current method has promise for high resolution genetic mapping studies, as physical location of features are well-defined on rice genome.
Kumar
Rajesh
R
Division of Plant Sciences, University of Missouri-Columbia, Columbia, Missouri, United States of America. kumarr@missouri.edu
Qiu
Jing
J
Joshi
Trupti
T
Valliyodan
Babu
B
Xu
Dong
D
Nguyen
Henry T
HT
D001483:Base Sequence; D018744:DNA, Plant / Q000235:genetics*; D018745:Genome, Plant; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000235:genetics*; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
D018744:DNA, Plant