title: Cytotoxicity of DP-bioglass paste used for treatment of dentin hypersensitivity.
abstract: DP-bioglass mixed with 30% phosphoric acid has been suggested to treat dentin hypersensitivity because the material is able to produce homogeneous occlusion of exposed dentinal tubules and is able to provide a sealing depth of up to 60 microm. The purpose of this study was to investigate the relative cytotoxic effect of the DP-bioglass paste, intermediate restorative material (IRM; DeTrey, Dentsply, Germany), and commercial desensitizing agent (Abmindent; Abmin Technologies Ltd, Turku, Finland) on human dental pulp cells by using a transwell insert model and a dentin-disc model. Cell viability was measured by means of a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results of this study indicated that cell viability for the DP-bioglass paste did not differ significantly from those of the IRM or Abmindent in the transwell insert model. In addition, MTT results of DP-bioglass paste were similar to the control group in the dentin-disc model. The results indicated that DP-bioglass paste was a highly biocompatible material and could potentially serve as an effective material for treatment of dentin hypersensitivity.
authors: Dental Department, Taiwan Adventist Hospital, Taipei, Taiwan.
authors: Tien-Chun
authors: TC
authors: Kuo
authors: Bor-Shiunn
authors: BS
authors: Lee
authors: Shu-Han
authors: SH
authors: Kang
authors: Feng-Huei
authors: FH
authors: Lin
authors: Chun-Pin
authors: CP
authors: Lin
mesh_terms: D000293:Adolescent; D000328:Adult; D001672:Biocompatible Materials / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004396:Coloring Agents; D003738:Dental Cements / Q000633:toxicity*; D003782:Dental Pulp / Q000187:drug effects*; D003804:Dentin / Q000187:drug effects*; D003807:Dentin Sensitivity / Q000517:prevention & control; D006801:Humans; D008422:Materials Testing; D008768:Methylmethacrylates / Q000633:toxicity; D013778:Tetrazolium Salts; D013844:Thiazoles; D015035:Zinc Oxide-Eugenol Cement / Q000633:toxicity
chemical_list: D001672:Biocompatible Materials; D004396:Coloring Agents; C418024:DP-bioactive glass paste; D003738:Dental Cements; D008768:Methylmethacrylates; D013778:Tetrazolium Salts; D013844:Thiazoles; D015035:Zinc Oxide-Eugenol Cement; C031667:IRM cement; C022616:thiazolyl blue