Liquid-phase microextraction combined with high-performance liquid chromatography for the enantioselective analysis of mefloquine in plasma samples.
A simple and rapid method, which involves liquid-phase microextraction (LPME) followed by HPLC analysis using Chiralpak AD column and UV detection, was developed for the enantioselective determination of mefloquine in plasma samples. Several factors that influence the efficiency of three-phase LPME were investigated and optimized. Under the optimal extraction conditions, the mean recoveries were 33.2 and 35.0% for (-)-(SR-)-mefloquine and (+)-(RS)-mefloquine, respectively. The method was linear over 50-1500 ng/ml range. Within-day and between-day assay precision and accuracy were below 15% for both enantiomers at concentrations of 150, 600 and 1200 ng/ml. Furthermore, no racemization or degradation were seen with the method described.
dos Santos Magalhães
Igor Rafael
IR
Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. do Café S/N, 14040-903 Ribeirão Preto, SP, Brazil.
Bonato
Pierina Sueli
PS
D000284:Administration, Oral; D000688:Amylose / Q000031:analogs & derivatives; D000818:Animals; D000962:Antimalarials / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D002623:Chemistry Techniques, Analytical / Q000379:methods* / Q000592:standards; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D006863:Hydrogen-Ion Concentration; D008297:Male; D015767:Mefloquine / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D000432:Methanol / Q000737:chemistry; D048448:Phenylcarbamates; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012965:Sodium Chloride / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D000962:Antimalarials; D048448:Phenylcarbamates; D012997:Solvents; C080033:Chiralpak AD; D012965:Sodium Chloride; D000688:Amylose; D015767:Mefloquine; D000432:Methanol