Protein micropatterning on bifunctional organic-inorganic sol-gel hybrid materials.
Active protein micropatterns and microarrays made by selective localization are popular candidates for medical diagnostics, such as biosensors, bioMEMS, and basic protein studies. In this paper, we present a simple fabrication process of thick (approximately 20 microm) protein micropatterning using capillary force lithography with bifunctional sol-gel hybrid materials. Because bifunctional sol-gel hybrid material can have both an amine function for linking with protein and a methacryl function for photocuring, proteins such as streptavidin can be immobilized directly on thick bifunctional sol-gel hybrid micropatterns. Another advantage of the bifunctional sol-gel hybrid materials is the high selective stability of the amine group on bifunctional sol-gel hybrid patterns. Because amine function is regularly contained in each siloxane oligomers, immobilizing sites for streptavidin are widely distributed on the surface of thick hybrid micropatterns. The micropatterning processes of active proteins using efficient bifunctional sol-gel hybrid materials will be useful for the development of future bioengineered systems because they can save several processing steps and reduce costs.
Kim
Woo-Soo
WS
Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon, 305-701, Republic of Korea.
Kim
Min-Gon
MG
Ahn
Jun-Hyeong
JH
Bae
Byeong-Soo
BS
Park
Chan Beum
CB
D019075:Electrophoresis, Capillary / Q000379:methods; D005782:Gels / Q000737:chemistry* / Q000528:radiation effects; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D015394:Molecular Structure; D010316:Particle Size; D040081:Protein Array Analysis / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012833:Siloxanes / Q000737:chemistry* / Q000528:radiation effects; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013499:Surface Properties; D014466:Ultraviolet Rays
D005782:Gels; D011506:Proteins; D012833:Siloxanes