title: Insecticide resistance in the malarial mosquito Anopheles arabiensis and association with the kdr mutation.
abstract: A colony of Anopheles arabiensis Patton (Diptera: Culicidae) from the Sennar region of Sudan was selected for resistance to dichlorodiphenyltrichloroethane (DDT). Adults from the F-16 generation of the resistant strain were exposed to all four classes of insecticides approved for use in malaria vector control and showed high levels of resistance to them all (24-h mortalities: malathion, 16.7%; bendiocarb, 33.3%; DDT, 12.1%; dieldrin, 0%; deltamethrin, 24.0%; permethrin, 0%). Comparisons between the unselected base colony and the DDT-resistant strain showed elevated glutathione-S-transferase (P<0.05) in both sexes and elevated esterases (P<0.05) in males only. The Leu-Phe mutation in the sodium channel gene was detected by polymerase chain reaction and sequencing, but showed no correlation with the resistant phenotype. These results do not provide any explanation as to why this colony exhibits such widespread resistance and further studies are needed to determine the precise mechanisms involved. The implications for malaria vector control in central Sudan are serious and resistance management (e.g. through the rotational use of different classes of insecticides) is recommended.
authors: Vector Control Reference Unit, National Institute for Communicable Diseases, NHLS, Sandringham, Johannesburg, South Africa.
authors: T S
authors: TS
authors: Matambo
authors: H
authors: H
authors: Abdalla
authors: B D
authors: BD
authors: Brooke
authors: L L
authors: LL
authors: Koekemoer
authors: A
authors: A
authors: Mnzava
authors: R H
authors: RH
authors: Hunt
authors: M
authors: M
authors: Coetzee
mesh_terms: D000110:Acetylcholinesterase / Q000032:analysis / Q000187:drug effects; D000367:Age Factors; D000818:Animals; D000852:Anopheles / Q000201:enzymology / Q000235:genetics*; D003634:DDT; D005260:Female; D005838:Genotype; D005982:Glutathione Transferase / Q000032:analysis; D007303:Insect Vectors / Q000201:enzymology / Q000235:genetics*; D007305:Insecticide Resistance / Q000235:genetics; D007306:Insecticides; D008297:Male; D009154:Mutation; D001074:Propoxur / Q000494:pharmacology
chemical_list: D007306:Insecticides; D001074:Propoxur; D003634:DDT; D005982:Glutathione Transferase; D000110:Acetylcholinesterase