title: Co-repressor SMRT and class II histone deacetylases promote Bach2 nuclear retention and formation of nuclear foci that are responsible for local transcriptional repression.
abstract: Bach2 is a member of the BTB-basic region leucine zipper factor family and represses transcription activity directed by the TPA response element, the Maf recognition element (MARE) and the antioxidant-responsive element. Recently, it was reported that upon oxidative stress Bach2 forms nuclear foci surrounding the promyelocytic leukaemia (PML) bodies and specifically represses the transcription around the PML bodies. Here we report that expression of the silencing mediator of retinoid and thyroid receptor (SMRT) and histone deacetylase4 (HDAC4) enhances the formation of the Bach2 foci in the nuclear matrix. SMRT mediates the HDAC4 binding to Bach2, and HDAC4 facilitates the retention of Bach2 in the foci. Scratch transcription labelling and 3D-reconstruction from the confocal images demonstrated that transcription is suppressed in and around the Bach2 foci. Indeed, Bach2 bound MARE and repressed the expression from the chromosomally integrated MARE-driven reporter gene when co-expressed with SMRT and HDAC4. Our observations suggest that both SMRT and HDAC4 play an important role in nuclear retention and the Bach2 focus formation in the mammalian cell nucleus, which may contribute to the local transcription repression.
authors: CREST Research Project, Japan Science and Technology Corp., Saitama 332-0012, Japan.
authors: Hideto
authors: H
authors: Hoshino
authors: Tomonori George
authors: TG
authors: Nishino
authors: Satoshi
authors: S
authors: Tashiro
authors: Masaya
authors: M
authors: Miyazaki
authors: Yoshihiro
authors: Y
authors: Ohmiya
authors: Kazuhiko
authors: K
authors: Igarashi
authors: Sueharu
authors: S
authors: Horinouchi
authors: Minoru
authors: M
authors: Yoshida
mesh_terms: D050976:Basic-Leucine Zipper Transcription Factors / Q000502:physiology*; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000502:physiology*; D006655:Histone Deacetylases / Q000502:physiology*; D006801:Humans; D015530:Nuclear Matrix / Q000502:physiology*; D009687:Nuclear Proteins / Q000502:physiology*; D056985:Nuclear Receptor Co-Repressor 2; D012097:Repressor Proteins / Q000502:physiology*; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: C491123:BACH2 protein, human; D050976:Basic-Leucine Zipper Transcription Factors; D004268:DNA-Binding Proteins; C539130:NCOR2 protein, human; D009687:Nuclear Proteins; D056985:Nuclear Receptor Co-Repressor 2; D012097:Repressor Proteins; C400300:HDAC4 protein, human; D006655:Histone Deacetylases