title: Proteome analysis of cerebrospinal fluid in Guillain-Barré syndrome (GBS).
abstract: We used two-dimensional difference in-gel electrophoresis (2-D-DIGE) for proteome analysis of cerebrospinal fluid (CSF) in Guillain-Barré syndrome (GBS). Spots showing >2-fold difference between GBS and controls were analysed using MALDI-TOF mass spectrometry. Proteins that were up-regulated in GBS included haptoglobin, serine/threonine kinase 10, alpha-1-antitrypsin, SNC73, alpha II spectrin, IgG kappa chain and cathepsin D preprotein, while transferrin, caldesmon, GALT, human heat shock protein 70, amyloidosis patient HL-heart-peptide 127aa and transthyretin were down-regulated. Some of these proteins are reported in CSF of GBS for the first time. Accordingly, the 2-D-DIGE technology may be useful to identify disease-specific proteins in patients with GBS.
authors: Department of Neurology, University of Ulm, Oberer Eselsberg 45, 89081 Ulm, Germany.
authors: V
authors: V
authors: Lehmensiek
authors: S D
authors: SD
authors: Süssmuth
authors: J
authors: J
authors: Brettschneider
authors: G
authors: G
authors: Tauscher
authors: S
authors: S
authors: Felk
authors: F
authors: F
authors: Gillardon
authors: H
authors: H
authors: Tumani
mesh_terms: D000328:Adult; D000368:Aged; D002555:Cerebrospinal Fluid / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D020275:Guillain-Barre Syndrome / Q000134:cerebrospinal fluid*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D008875:Middle Aged; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D020543:Proteome