title: Thickening of the peritubular capillary basement membrane is a useful diagnostic marker of chronic rejection in renal allografts.
abstract: In kidney transplantation, the multilayering of the peritubular capillary basement membrane (MLPTC) in electron microscopy (EM) has been recognized as a feature of chronic rejection (CR). In this study, thickening of the peritubular capillary (PTC) basement membrane was evaluated by light microscopy (LM) to determine whether it corresponds to the MLPTC in EM and whether it can be used as a diagnostic marker of CR. Forty-eight patients with late renal allograft were divided into chronic allograft nephropathy (CAN) with CR (Group 1, n = 23), CAN without CR (Group 2, n = 19) and CAN-free (Group 3, n = 6). The thickening of the PTC basement membrane (ptcbm) was scored from grades 0 to 2 (ptcbm score), and the MLPTC thickness was measured in EM. Interobserver agreement on ptcbm scores was statistically significant (Kappa coefficient = 0.63). LM and EM lesions corresponded very well. The ptcbm score was highest in Group 1, and ptcbm2 corresponded closely with CR. Group 1 showed significantly thicker MLPTC than Groups 2 and 3. The results validated the usefulness of the ptcbm score and suggested that the thickening of the PTC basement membrane can be a novel diagnostic marker of CR.
authors: Department of Molecular Pathology, Institute of Basic Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki, Japan. kumiaita@md.tsukuba.ac.jp
authors: K
authors: K
authors: Aita
authors: Y
authors: Y
authors: Yamaguchi
authors: S
authors: S
authors: Horita
authors: M
authors: M
authors: Ohno
authors: K
authors: K
authors: Tanabe
authors: S
authors: S
authors: Fuchinoue
authors: S
authors: S
authors: Teraoka
authors: H
authors: H
authors: Toma
authors: M
authors: M
authors: Nagata
mesh_terms: D000328:Adult; D001485:Basement Membrane / Q000473:pathology*; D015415:Biomarkers / Q000032:analysis; D002196:Capillaries / Q000473:pathology*; D005260:Female; D005500:Follow-Up Studies; D006084:Graft Rejection / Q000145:classification / Q000473:pathology*; D006801:Humans; D016030:Kidney Transplantation / Q000473:pathology*; D007684:Kidney Tubules / Q000098:blood supply* / Q000473:pathology*; D008297:Male; D008853:Microscopy / Q000379:methods; D008875:Middle Aged; D013997:Time Factors; D014184:Transplantation, Homologous / Q000473:pathology
chemical_list: D015415:Biomarkers