title: Superparamagnetic iron oxide labeling and transplantation of adipose-derived stem cells in middle cerebral artery occlusion-injured mice.
abstract: OBJECTIVE
  Adipose-derived stem cells are an alternative stem cell source for CNS therapies. The goals of the current study were to label adipose-derived stem cells with superparamagnetic iron oxide (SPIO) particles, to use MRI to guide the transplantation of adipose-derived stem cells in middle cerebral artery occlusion (MCAO)-injured mice, and to localize donor adipose-derived stem cells in the injured brain using MRI. We hypothesized that we would successfully label adipose-derived stem cells and image them with MRI.
  
  
  METHODS
  Adipose-derived stem cells harvested from mice inbred for green fluorescent protein were labeled with SPIO ferumoxide particles through the use of poly-L-lysine. Adipose-derived stem cell viability, iron staining, and proliferation were measured after SPIO labeling, and the sensitivity of MRI in the detection of SPIO-labeled adipose-derived stem cells was assessed ex vivo. Adult mice (n = 12) were subjected to unilateral MCAO. Two weeks later, in vivo 7-T MRI was performed to guide stereotactic transplantation of SPIO-labeled adipose-derived stem cells into brain tissue adjacent to the infarct. After 24 hours, the mice were sacrificed for high-resolution ex vivo 7-T or 9.4-T MRI and histologic study.
  
  
  RESULTS
  Adipose-derived stem cells were efficiently labeled with SPIO particles without loss of cell viability or proliferation. Using MRI, we guided precise transplantation of adipose-derived stem cells. MR images of mice given injections of SPIO-labeled adipose-derived stem cells had hypointense regions that correlated with the histologic findings in donor cells.
  
  
  CONCLUSIONS
  MRI proved useful in transplantation of adipose-derived stem cells in vivo. This imaging technique may be useful for studies of CNS stem cell therapies.
authors: Department of Surgery, Duke University Medical Center, Box 3815, Durham, NC 27710, USA.
authors: Henry E
authors: HE
authors: Rice
authors: Edward W
authors: EW
authors: Hsu
authors: Huaxin
authors: H
authors: Sheng
authors: Debra A
authors: DA
authors: Evenson
authors: Alex J
authors: AJ
authors: Freemerman
authors: Kristine M
authors: KM
authors: Safford
authors: James M
authors: JM
authors: Provenzale
authors: David S
authors: DS
authors: Warner
authors: G Allan
authors: GA
authors: Johnson
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002545:Brain Ischemia / Q000175:diagnosis* / Q000209:etiology / Q000601:surgery*; D003287:Contrast Media; D003911:Dextrans; D052203:Ferrosoferric Oxide; D020244:Infarction, Middle Cerebral Artery / Q000150:complications*; D007501:Iron; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles; D051379:Mice; D008810:Mice, Inbred C57BL; D010087:Oxides; D033581:Stem Cell Transplantation / Q000379:methods*
chemical_list: D003287:Contrast Media; D003911:Dextrans; D058185:Magnetite Nanoparticles; D010087:Oxides; C097921:ferumoxtran-10; D007501:Iron; D052203:Ferrosoferric Oxide