title: Preparation and observation of fresh-frozen sections of the green fluorescent protein transgenic mouse head.
abstract: Hard tissue decalcification can cause variation in the constituent protein characteristics. This paper describes a method of preparating of frozen mouse head sections so as to clearly observe the nature of the constituent proteins. Frozen sections of various green fluorescent protein (GFP) transgenic mouse heads were prepared using the film method developed by Kawamoto and Shimizu. This method made specimen dissection without decalcification possible, wherein GFP was clearly observed in an undamaged state. Conversely, using the same method with decalcification made GFP observation in the transgenic mouse head difficult. This new method is suitable for observing GFP marked cells, enabling us to follow the transplanted GFP marked cells within frozen head sections.
authors: Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Toyama 930-0194, Japan.
authors: Masahito
authors: M
authors: Tada
authors: Yoshinori
authors: Y
authors: Shinohara
authors: Ichiro
authors: I
authors: Kato
authors: Koichi
authors: K
authors: Hiraga
authors: Tomoyasu
authors: T
authors: Aizawa
authors: Makoto
authors: M
authors: Demura
authors: Yoshihiro
authors: Y
authors: Mori
authors: Hiroyuki
authors: H
authors: Shinoda
authors: Mineyuki
authors: M
authors: Mizuguchi
authors: Keiichi
authors: K
authors: Kawano