title: A novel method for determination of protein in human serum utilizing the trihydroxylphenylfluorone-molybdenum(VI) complex as a probe by fluorescence spectrometry.
abstract: The fluorescence intensity of the trihydroxyl-phenylfluorone-molybdenum(VI) [Mo(VI)] complex is quenched by protein. Based on this, a novel method for protein assay in aqueous solution was developed. With pH 3.75 acetic acid-sodium acetate buffer solution, in the presence of p-octyl polyethylene glycol phenyl ether microemulsion, the quenched fluorescence intensity is proportional to the concentration of bovine serum albumin (BSA) in the range of 0-7.00 microg/mL, and the detection limit of BSA is 5.65 ng/mL. There is no interference from amino acids and most metal ions. The method developed in this paper has been used for the successful determination of protein in human serum.
authors: Jinan University, School of Chemistry and Chemical Engineering, Jinan 250022, People's Republic of China (PRC).
authors: Qin
authors: Q
authors: Wei
authors: Ma
authors: M
authors: Hongmin
authors: Caihong
authors: C
authors: Duan
authors: Jin
authors: J
authors: Wang
authors: Shuyuan
authors: S
authors: Liu
authors: Bin
authors: B
authors: Du
mesh_terms: D001555:Benzene Derivatives; D001798:Blood Proteins / Q000032:analysis* / Q000302:isolation & purification; D004655:Emulsions; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007700:Kinetics; D008982:Molybdenum; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D001555:Benzene Derivatives; D001798:Blood Proteins; D004655:Emulsions; D007202:Indicators and Reagents; D008982:Molybdenum