Sense-antisense pairs in mammals: functional and evolutionary considerations.
BACKGROUND
A significant number of genes in mammalian genomes are being found to have natural antisense transcripts (NATs). These sense-antisense (S-AS) pairs are believed to be involved in several cellular phenomena.


RESULTS
Here, we generated a catalog of S-AS pairs occurring in the human and mouse genomes by analyzing different sources of expressed sequences available in the public domain plus 122 massively parallel signature sequencing (MPSS) libraries from a variety of human and mouse tissues. Using this dataset of almost 20,000 S-AS pairs in both genomes we investigated, in a computational and experimental way, several putative roles that have been assigned to NATs, including gene expression regulation. Furthermore, these global analyses allowed us to better dissect and propose new roles for NATs. Surprisingly, we found that a significant fraction of NATs are artifacts produced by genomic priming during cDNA library construction.


CONCLUSIONS
We propose an evolutionary and functional model in which alternative polyadenylation and retroposition account for the origin of a significant number of functional S-AS pairs in mammalian genomes.
Galante
Pedro A F
PA
Ludwig Institute for Cancer Research, São Paulo Branch, Hospital Alemão Oswaldo Cruz, São Paulo, SP, Brazil. pgalante@compbio.ludwig.org.br
Vidal
Daniel O
DO
de Souza
Jorge E
JE
Camargo
Anamaria A
AA
de Souza
Sandro J
SJ
D000818:Animals; D005075:Biological Evolution; D019295:Computational Biology; D005786:Gene Expression Regulation; D015723:Gene Library; D016678:Genome; D006801:Humans; D008322:Mammals / Q000235:genetics; D051379:Mice; D026723:Polyadenylation; D016372:RNA, Antisense; D012333:RNA, Messenger / Q000235:genetics*; D018626:Retroelements
D016372:RNA, Antisense; D012333:RNA, Messenger; D018626:Retroelements