[Cloning and the sequence analysis of the fish glutathione transferase Pi gene].
Using RT-PCR method, the glutathione transferase Pi cDNAs were cloned from Cyprinus carpio, Hypophthalmichthys molitrix, and Carassius auratus. The open reading frames (ORFs) from the 3 fishes were 627 bp long (encoding for 208 amino acids) with the initial code ATG and the terminal code TGA. The sequence similarity was 50% between fish and mammals, 33% between fish and amphibian, and 15% between fish and arthropoda, respectively. The sequence similarity was big among fishes, and the average value of the 4 cyprinids was about 85%. Phylogenetic tree was constructed for 13 species based on GST Pi amino acid sequences using MP (Maximum Parsimony) method. Two major clusters were recognized: cluster one consisted of Mammals (bootstrap 100) and cluster two consisted of fishes (bootstrap 93). Based on the sequences analyses of N/C domain of GST Pi, we proposed the detoxification mechanism of freshwater fishes that were thought to have stronger tolerance to microcystins.
Sun
Yu-Hua
YH
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Education Ministry, Huazhong Agricultural University, Wuhan, China. yhsun67@sina.com
Xie
Ping
P
Guo
Hai-Tao
HT
Xia
Wen-Wei
WW
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D005399:Fishes / Q000235:genetics*; D051549:Glutathione S-Transferase pi / Q000145:classification* / Q000235:genetics*; D005982:Glutathione Transferase / Q000235:genetics; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid
D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase