title: Accumulation of triosephosphate isomerase, with sequence homology to Beta amyloid peptides, in vessel walls of the newborn piglet hippocampus.
abstract: We investigated whether beta-amyloid (Abeta)-like immunoreactivity was seen in the brains of newborn piglets. The immunoreactivity for Abeta(1-42) and Abeta(1-40) proteins, but not Abeta precursor protein, was present in CD68-positive perivascular cells of the hippocampus and in parts of the meninges. It was colocalized with immunoreactivity for receptor for advanced glycation end product and tumor necrosis factor-alpha. The protein with a molecular mass of 27 kDa, which was recognized by the Abeta antibodies, was identified as triosephosphate isomerase (TPI) with sequence homology to Abeta peptides by N-terminal amino acid sequencing, mass fingerprint analysis using matrix-associated laser desorption/ionization mass spectrometry, and Western blotting. Western blotting assay also revealed that detectable expression of Abeta proteins were not seen in the piglet brains. These findings indicate that TPI with sequence homology to Abeta peptides accumulates in perivascular cells of the microglia/macrophage lineage located around arterial vessels of the newborn piglet hippocampus.
authors: Maternal and Perinatal Center, Faculty of Medicine, Kagawa University, Miki-cho 1750-1, Kita-gun, Kagawa, Japan.
authors: Takashi
authors: T
authors: Kusaka
authors: Masaki
authors: M
authors: Ueno
authors: Takanori
authors: T
authors: Miki
authors: Kenji
authors: K
authors: Kanenishi
authors: Yukiko
authors: Y
authors: Nagai
authors: Cheng-Long
authors: CL
authors: Huang
authors: Yasuo
authors: Y
authors: Okamoto
authors: Takafumi
authors: T
authors: Ogawa
authors: Masayuki
authors: M
authors: Onodera
authors: Susumu
authors: S
authors: Itoh
authors: Ichiro
authors: I
authors: Akiguchi
authors: Haruhiko
authors: H
authors: Sakamoto
mesh_terms: D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000737:chemistry*; D000818:Animals; D001808:Blood Vessels / Q000201:enzymology*; D015153:Blotting, Western; D006624:Hippocampus / Q000098:blood supply* / Q000201:enzymology*; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013552:Swine; D014305:Triose-Phosphate Isomerase / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D016229:Amyloid beta-Peptides; D014305:Triose-Phosphate Isomerase