Extracellular carbonic anhydrases of the stromatolite-forming cyanobacterium Microcoleus chthonoplastes.
Active extracellular carbonic anhydrases (CAs) were found in the alkaliphilic stromatolite-forming cyanobacterium Microcoleus chthonoplastes. Enzyme activity was detected in intact cells and in the cell envelope fraction. Western blot analysis of polypeptides from the cell envelope suggested the presence of at least two polypeptides cross-reacting with antibodies against both alpha and beta classes of CA. Immunocytochemical analysis revealed putative alpha-CA localized in the glycocalyx. This alpha-CA has a molecular mass of about 34 kDa and a pI of 3.5. External CAs showed two peaks of activity at around pH 10 and 7.5. The possible involvement of extracellular CAs of M. chthonoplastes in photosynthetic assimilation of inorganic carbon and its relationship to CaCO(3) deposition during mineralization of cyanobacterial cells are discussed.
Kupriyanova
Elena
E
Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow, 127276 Russia.
Villarejo
Arsenio
A
Department of Biology, Universidad Autonoma de Madrid, 28049 Madrid.|Umeå Plant Science Centre, Department of Plant Physiology, University of Umeå, S-901 87 Umeå, Sweden.
Markelova
Alexandra
A
Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow, 127276 Russia.
Gerasimenko
Lyudmila
L
Institute of Microbiology, Russian Academy of Sciences, Prospect 60-Letiya Oktyabrya 7/2, Moscow, 117312 Russia.
Zavarzin
Georgy
G
Institute of Microbiology, Russian Academy of Sciences, Prospect 60-Letiya Oktyabrya 7/2, Moscow, 117312 Russia.
Samuelsson
Göran
G
Umeå Plant Science Centre, Department of Plant Physiology, University of Umeå, S-901 87 Umeå, Sweden.
Los
Dmitry A
DA
Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow, 127276 Russia.
Pronina
Natalia
N
Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow, 127276 Russia.
D015153:Blotting, Western; D002119:Calcium Carbonate / Q000378:metabolism; D002256:Carbonic Anhydrases / Q000378:metabolism*; D000458:Cyanobacteria / Q000737:chemistry / Q000166:cytology / Q000201:enzymology*; D015180:Electrophoresis, Gel, Two-Dimensional; D019276:Glycocalyx / Q000737:chemistry
D002256:Carbonic Anhydrases; D002119:Calcium Carbonate