title: The pattern of immune cell infiltration in chromoblastomycosis: involvement of macrophage inflammatory protein-1 alpha/CCL3 and fungi persistence.
abstract: Chromoblastomycosis (CR) is a subcutaneous chronic mycosis characterized by a granulomatous inflammatory response. However, little is known regarding the pattern of leukocyte subsets in CR and the pathways involved in their recruitment. The objective of this study was to assess the cellular subsets, chemokine, chemokine receptors and enzymes in CR. The inflammatory infiltrate was characterized by immunohistochemistry using antibodies against macrophages (CD68), Langerhans'cells (S100), lymphocytes (CD3, CD4, CD8, CD45RO, CD20 and CD56) and neutrophils (CD15). The expression of MIP-1alpha (Macrophage inflammatory protein-1alpha), chemokine receptors (CXCR3 and CCR1) and enzymes (superoxide dismutase-SOD and nitric oxide synthase-iNOS) was also evaluated by the same method. We observed an increase in all populations evaluated when compared with the controls. Numbers of CD15(+) and CD56(+) were significantly lower than CD3(+), CD4(+), CD20(+) and CD68(+) cells. Statistical analysis revealed an association of fungi numbers with CD3, CD45RO and iNOS-positive cells. Furthermore, MIP-1alpha expression was associated with CD45RO, CD68, iNOS and CXCR3. Our results suggest a possible role of MIP-1alpha and fungi persistence in the cell infiltration in CR sites.
authors: Cell Biology Department, Institute of Biology, Universidade de Brasília, Brasília, Distrito Federal, Brazil.
authors: Vanuza Cristina
authors: VC
authors: Sá
authors: Tarcília Aparecida
authors: TA
authors: Silva
authors: Carmelia Matos Santiago
authors: CM
authors: Reis
authors: Fernando Queiroz
authors: FQ
authors: Cunha
authors: Florêncio
authors: F
authors: Figueiredo
authors: Anamélia Lorenzetti
authors: AL
authors: Bocca
mesh_terms: D000368:Aged; D015415:Biomarkers; D016022:Case-Control Studies; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D002862:Chromoblastomycosis / Q000201:enzymology / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular; D007801:Langerhans Cells / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D019402:Macrophage Inflammatory Proteins / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D008875:Middle Aged; D009504:Neutrophils / Q000276:immunology; D019001:Nitric Oxide Synthase / Q000276:immunology; D019707:Receptors, Chemokine / Q000276:immunology*; D013482:Superoxide Dismutase / Q000276:immunology
chemical_list: D015415:Biomarkers; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D019402:Macrophage Inflammatory Proteins; D019707:Receptors, Chemokine; D019001:Nitric Oxide Synthase; D013482:Superoxide Dismutase