Application of protein-fragment complementation assays in cell biology.
We have developed a general experimental strategy that enables the quantitative detection of dynamic protein-protein interactions in intact living cells, based on protein-fragment complementation assays (PCAs). In this method, protein-protein interactions are coupled to refolding of enzymes from cognate fragments where reconstitution of enzyme activity acts as the detector of a protein interaction. Here we discuss the application of PCA to different aspects of cell biology.
Remy
Ingrid
I
Département de Biochimie, Université de Montréal, Succursale Center-Ville, Montréal, QC, Canada.
Michnick
Stephen W
SW
D001669:Biochemical Phenomena; D001671:Biochemistry; D003585:Cell Biology; D011506:Proteins / Q000737:chemistry*
D011506:Proteins