title: Effects of Nidus Vespae extract and chemical fractions on glucosyltransferases, adherence and biofilm formation of Streptococcus mutans.
abstract: Nidus Vespae (the honeycomb of Polistes olivaceous, P. japonicus Saussure and Parapolybiavaria fabricius) have been extensively used in traditional Chinese medicine, given their multiple pharmacological activities, including antimicrobial, anti-inflammatory, anti-virus, anti-tumor and anesthetic properties. The present study evaluated the anti-glucosyltransferases (GTFs) activity, anti-adherence and anti-biofilm properties of 95% ethanol/water extract, cyclohexane/ethyl acetate, petroleum ether/ethyl acetate and chloroform/methanol fractions of Nidus Vespae. Chloroform/methanol fraction showed a remarkable capacity for inhibiting the adherence of Streptococcus mutans ATCC 25175 to saliva-coated hydroxyapatite disc (S-HA) at sub-MC concentrations. In addition, the Nidus Vespae extract and chemical fractions significantly inhibited the activity of cell-associated and extracellular GTFs at sub-MIC concentrations, and the chloroform/methanol fraction was the most effective one. For the anti-biofilm activity assays, minimum biofilm inhibition concentrations (MBIC50) and minimum biofilm reduction concentrations (MBRC50) were determined using the microdilution method. The chloroform/methanol fraction showed the highest anti-biofilm activities with a MBIC50 of 8mg/ml and a MBRC(50) of 16mg/ml against Streptococcus mutans ATCC 25175. The significant inhibition of GTFs activity and biofilm formation demonstrated by Nidus Vespae shows it to be a promising natural product for the prevention of dental caries.
authors: Key Laboratory for Oral Biomedical Engineering Ministry of Education, Chengdu, China.
authors: Jin
authors: J
authors: Xiao
authors: Yuling
authors: Y
authors: Zuo
authors: Yuan
authors: Y
authors: Liu
authors: Jiyao
authors: J
authors: Li
authors: Yuqing
authors: Y
authors: Hao
authors: Xuedong
authors: X
authors: Zhou
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects*; D018441:Biofilms / Q000187:drug effects*; D002710:Chlorhexidine / Q000494:pharmacology; D044622:Dental Pellicle / Q000382:microbiology; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D017886:Durapatite / Q000737:chemistry; D005936:Glucans / Q000032:analysis; D005964:Glucosyltransferases / Q000187:drug effects*; D006801:Humans; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D011135:Polysaccharides, Bacterial / Q000187:drug effects; D013295:Streptococcus mutans / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology; D014863:Wasps; D014885:Waxes / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D000891:Anti-Infective Agents, Local; D004365:Drugs, Chinese Herbal; D005936:Glucans; D010936:Plant Extracts; D011135:Polysaccharides, Bacterial; D014885:Waxes; D017886:Durapatite; D005964:Glucosyltransferases; D002710:Chlorhexidine