title: Identification and characterization of ERp29 in rat spermatozoa during epididymal transit.
abstract: The mammalian epididymis is able to create sequential changes in the composition of luminal fluid throughout its length, wherein spermatozoa undergo morphological, biochemical, and physiological modifications. Subsequently, spermatozoa acquire the ability for fertilization upon reaching the epididymal cauda. In this study, protein variations in Sprague-Dawley rat spermatozoa along the caput and caudal regions of epididymis were investigated by high-resolution two-dimensional gel electrophoresis (2DE) in combination with mass spectrometry. From total protein spots on the 2DE maps, 43 spots were shown to be significantly modified as sperm traverse the epididymis, and seven unambiguous proteins were identified from them. Finally, using indirect immunofluorescence, we demonstrated that localization of one of these seven proteins, the endoplasmic reticulum protein (ERp29) precursor, which was first reported in mammalian spermatozoa, was apparently up-regulated as the sperm underwent epididymal maturation and expressed mainly on caudal sperm. Western blot analysis also revealed that ERp29 precursor, from both whole spermatozoa and membrane proteins, increased significantly as the sperm underwent epididymal maturation. Furthermore, the results from immunofluorescence-stained epididymal frozen sections demonstrated that ERp29 was localized in cytoplasm of epididymal epithelia, and the fluorescence intensity was significantly higher in the caudal epididymis than in the caput. These clues indicated that the ERp29 precursor, perhaps related to secretory protein synthesis and absorbed by spermatozoa, may play a vital role in sperm maturation during the epididymal transit, particularly, in the sperm/organelle membrane.
authors: Shanghai Key Laboratory for Reproductive Medicine, Histology and Embryology, School of Medicine, Shanghai Jiao Tong University, No. 280, Chong Qing Rd. (South), Shanghai 200025, China.
authors: Wei
authors: W
authors: Guo
authors: Fei
authors: F
authors: Qu
authors: Li
authors: L
authors: Xia
authors: Qiangsu
authors: Q
authors: Guo
authors: Xiaoqian
authors: X
authors: Ying
authors: Zhide
authors: Z
authors: Ding
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional; D004822:Epididymis; D005455:Fluorescent Antibody Technique; D006360:Heat-Shock Proteins / Q000032:analysis / Q000302:isolation & purification*; D007525:Isoelectric Focusing; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013083:Sperm Transport / Q000502:physiology*; D013094:Spermatozoa / Q000737:chemistry*
chemical_list: C110909:Erp29 protein, rat; D006360:Heat-Shock Proteins