title: Promoter analysis of human CC chemokine CCL23 gene in U937 monocytoid cells.
abstract: Expression of CCL23 is induced by external stimuli including PMA in monocytes, but its transcriptional regulation has not been studied to date. Serial deletion analysis of its 5' flanking region revealed that the region -293 to +31 was important for induction by PMA. Cis-acting elements at the -269/-264 (NFAT site), -167/-159 (NF-kappaB site), and -51/-43 (AP-1 site) positions were identified as the critical sites for the CCL23 expression in U937 cells. We demonstrated the binding of the transcription factors to the consensus sites. Specific inhibitors for signal pathways reduced PMA-induced expression of CCL23, confirming involvement of these transcription factors.
authors: Graduate School of Biotechnology and Institute of Life Sciences and Resources, Kyung Hee University, Yongin 449-701, Korea.
authors: Yong-Hyun
authors: YH
authors: Shin
authors: Guy Wilhem
authors: GW
authors: Lee
authors: Kyung-No
authors: KN
authors: Son
authors: Sang Min
authors: SM
authors: Lee
authors: Chang Joong
authors: CJ
authors: Kang
authors: Byoung S
authors: BS
authors: Kwon
authors: Jiyoung
authors: J
authors: Kim
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D002273:Carcinogens / Q000494:pharmacology; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D019742:Chemokines, CC / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D050778:NFATC Transcription Factors / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011485:Protein Binding; D012045:Regulatory Sequences, Nucleic Acid; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D018808:Transcription Factor AP-1 / Q000378:metabolism; D014158:Transcription, Genetic; D020298:U937 Cells
chemical_list: C105504:CCL23 protein, human; D002273:Carcinogens; D019742:Chemokines, CC; D016328:NF-kappa B; D050778:NFATC Transcription Factors; D018808:Transcription Factor AP-1; D013755:Tetradecanoylphorbol Acetate