title: CRISPR provides acquired resistance against viruses in prokaryotes.
abstract: Clustered regularly interspaced short palindromic repeats (CRISPR) are a distinctive feature of the genomes of most Bacteria and Archaea and are thought to be involved in resistance to bacteriophages. We found that, after viral challenge, bacteria integrated new spacers derived from phage genomic sequences. Removal or addition of particular spacers modified the phage-resistance phenotype of the cell. Thus, CRISPR, together with associated cas genes, provided resistance against phages, and resistance specificity is determined by spacer-phage sequence similarity.
authors: Danisco USA Inc., 3329 Agriculture Drive, Madison, WI 53716, USA.
authors: Rodolphe
authors: R
authors: Barrangou
authors: Christophe
authors: C
authors: Fremaux
authors: Hélène
authors: H
authors: Deveau
authors: Melissa
authors: M
authors: Richards
authors: Patrick
authors: P
authors: Boyaval
authors: Sylvain
authors: S
authors: Moineau
authors: Dennis A
authors: DA
authors: Romero
authors: Philippe
authors: P
authors: Horvath
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics; D021901:DNA, Intergenic / Q000235:genetics*; D019143:Evolution, Molecular; D005798:Genes, Bacterial; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009154:Mutation; D020641:Polymorphism, Single Nucleotide; D012091:Repetitive Sequences, Nucleic Acid; D017108:Streptococcus Phages / Q000235:genetics / Q000502:physiology*; D048249:Streptococcus thermophilus / Q000235:genetics* / Q000821:virology*; D010948:Viral Plaque Assay; D014779:Virus Replication
chemical_list: D004269:DNA, Bacterial; D021901:DNA, Intergenic