Wangia profunda gen. nov., sp. nov., a novel marine bacterium of the family Flavobacteriaceae isolated from southern Okinawa Trough deep-sea sediment.
An orange-pigmented, Gram-negative, nonmotile, strictly aerobic and oxidase- and catalase-positive bacterium (SM-A87(T)) was isolated from the deep-sea sediment of the southern Okinawa Trough area. The main fatty acids were i15 : 0, i17 : 0 3OH, i15 : 1 G, i17 : 1 omega 9c, 15 : 0, i15 : 0 3OH and summed feature 3 (comprising i-15 : 0 2OH and/or 16 : 1 omega 7c). MK-6 was the predominant respiratory quinone. DNA G+C content was 35.8 mol%. Flexirubin-type pigments were absent. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain SM-A87(T) formed a distinct lineage within the family Flavobacteriaceae, with <93% sequence similarity to the nearest strain of genus Salegentibacter. Moreover, strain SM-A87(T) could be distinguished from the nearest phylogenetic neighbors by a number of chemotaxonomic and phenotypic properties. On the basis of polyphasic analyses, it is proposed that strain SM-A87(T) be classified in a novel genus and a new species in the family Flavobacteriaceae, designated Wangia profunda gen. nov., sp. nov. The type strain is SM-A87(T) (CCTCC AB 206139(T)=DSM 18752).
Qin
Qi-Long
QL
The State Key Lab of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan, China.
Zhao
Dian-Li
DL
Wang
Jing
J
Chen
Xiu-Lan
XL
Dang
Hong-Yue
HY
Li
Tie-Gang
TG
Zhang
Yu-Zhong
YZ
Gao
Pei-Ji
PJ
D000332:Aerobiosis; D002374:Catalase / Q000096:biosynthesis; D002681:China; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D041965:Flavobacteriaceae / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology; D020459:Genes, rRNA / Q000235:genetics; D019015:Geologic Sediments / Q000382:microbiology*; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000096:biosynthesis; D010641:Phenotype; D010802:Phylogeny; D010860:Pigments, Biological / Q000096:biosynthesis; D011809:Quinones / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D004269:DNA, Bacterial; D005227:Fatty Acids; D010860:Pigments, Biological; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D010088:Oxidoreductases; D002374:Catalase