title: Molecular investigations of an outbreak of parainfluenza virus type 3 and respiratory syncytial virus infections in a hematology unit.
abstract: A large simultaneous outbreak of respiratory syncytial virus (RSV) and parainfluenza type 3 (PIV-3) infections occurred on an adult hematology unit. Implementation of enhanced infection control was complicated by cocirculation of the two different viruses, with prolonged viral shedding from infected patients, and placed great pressure on health care staff; of 27 infected hematopoietic stem cell transplant patients, 9 died, and the unit was closed for 2 months. Retrospective molecular investigation of the virus strains involved in the outbreak was performed by analyzing part of the fusion gene of PIV-3 and part of the glycoprotein gene of RSV. Reverse transcription-PCR on nasopharyngeal aspirates from patients infected before and during the simultaneous outbreak generated amplicons for sequence analysis. A single strain of RSV and a single strain of PIV-3 had spread from person to person within the unit; 7 patients were infected with RSV, 22 were infected with PIV-3, and 4 were infected with both viruses. The PIV-3 outbreak had started at the beginning of August 3 months before the RSV outbreak; it had arisen when PIV-3 was introduced from the community by a patient and passed to another patient, who became chronically infected with the identical strain and, in spite of being nursed in isolation, was most likely the source from which widespread infection occurred in November. Had these early cases been linked to a common PIV-3 strain at the time of diagnosis, enhanced infection control precautions might have prevented the eventual extensive spread of PIV-3, making it much easier to deal with the later RSV outbreak.
authors: Centre for Virology, Department of Infection, Royal Free & University College Medical School (UCL Campus), Windeyer Institute of Medical Sciences, London W1T 4JF, United Kingdom.
authors: Hamid
authors: H
authors: Jalal
authors: David F
authors: DF
authors: Bibby
authors: Julie
authors: J
authors: Bennett
authors: Rebecca E
authors: RE
authors: Sampson
authors: Nicola S
authors: NS
authors: Brink
authors: Stephen
authors: S
authors: MacKinnon
authors: Richard S
authors: RS
authors: Tedder
authors: Katherine N
authors: KN
authors: Ward
mesh_terms: D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D003428:Cross Infection / Q000453:epidemiology / Q000401:mortality / Q000821:virology; D004196:Disease Outbreaks; D006405:Hematology; D006757:Hospital Units; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D010224:Parainfluenza Virus 3, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012367:RNA, Viral / Q000302:isolation & purification; D018357:Respiratory Syncytial Virus Infections / Q000453:epidemiology* / Q000401:mortality / Q000821:virology; D018113:Respiratory Syncytial Virus, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010253:Respirovirus Infections / Q000453:epidemiology* / Q000401:mortality / Q000821:virology; D017422:Sequence Analysis, DNA
chemical_list: D012367:RNA, Viral