Synthesis, platelet adhesion and cytotoxicity studies of new glycerophosphoryl-containing polyurethanes.
In this work we synthesized new MDI -based poly(ether)urethanes (PEUs) with phospholipid-like residue as chain extender. Polymers were prepared by a conventional two-step solution polymerization procedure using 4,4' diphenylmethanediisocyanate (MDI) and poly(1,4- butanediol) with 1000 as molecular weight to form prepolymers which were successively polymerized with 1 glycerophosphorylcholine (1-GPC), 2-glycerophosphorylcholine (2-GPC) or glycerophosphorylserine (GPS) as chain extenders. Two reference polymers bearing 1,4-butandiol (BD) have been also synthesized. The polymers obtained were characterized by Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), differential scanning calorimetry (DSC) and modulated scanning calorimetry (MDSC). The biocompatibility of synthesized segmented polyurethanes was then investigated by platelet-rich plasma contact studies and related scanning electron microscopy (SEM) photographs for blood compatibility and cytotoxicity assay (MTT test) on material elution to assess the effect of any toxic leachables on cellular viability. Three polymers among all have given very satisfactory results suggesting to investigate more deeply their possible use in biomedical devices.
D'Arrigo
P
P
Dipartimento di Chimica, Materiali ed Ingegneria Chimica Giulio Natta, Politecnico di Milano, Milano, Italy.
Giordano
C
C
Macchi
P
P
Malpezzi
L
L
Pedrocchi-Fantoni
G
G
Servi
S
S
D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity; D005997:Glycerylphosphorylcholine / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity; D010768:Phosphoserine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D010973:Platelet Adhesiveness / Q000187:drug effects*; D011140:Polyurethanes / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity
D001672:Biocompatible Materials; D011140:Polyurethanes; D010768:Phosphoserine; C067651:glycerophosphoserine; D005997:Glycerylphosphorylcholine