Spin state selective Hadamard encoding during transfer periods using multiple selective CW-HCP.
Hadamard spectroscopy today represents an alternative to conventional Fourier transform spectroscopy. The selective inversion of several narrow frequency bands is typically achieved by taylored inversion pulses in place of t1-evolution periods. However, band-selective inversion can also be achieved during coherence transfer steps, thereby shortening the period during which the magnetization is in the transverse plane. Using CW heteronuclear cross polarization (CW-HCP) as an example for highly selective coherence transfer, the implementation of Hadamard encoding within a transfer step is presented. Transfer characteristics, the preparation of multiple frequency selective CW-HCP and the possibility of acquiring spin state selective spectra are discussed in detail. The theoretical results are verified on two examples involving a cyclic pentapeptide and ubiquitin.
Kobzar
Kyryl
K
Department Chemie, Organische Chemie II, Technische Universität München, Lichtenbergstrasse 4, D-85747 Garching, Germany.
Luy
Burkhard
B
D016011:Normal Distribution; D010455:Peptides / Q000737:chemistry; D013057:Spectrum Analysis / Q000295:instrumentation* / Q000379:methods*; D019097:Spin Trapping / Q000379:methods*; D025801:Ubiquitin / Q000737:chemistry
D010455:Peptides; D025801:Ubiquitin