title: Dissemination and persistence of a plasmid-mediated TEM-3-like beta-lactamase, TEM-139, among Enterobacteriaceae in Bulgaria.
abstract: During a survey of extended-spectrum beta-lactamases (ESBLs) in Bulgaria from 1996 to 2003, a TEM-3-like ESBL was detected in strains of Klebsiella pneumoniae, Escherichia coli, Citrobacter freundii and Klebsiella oxytoca from three centres in three different towns. The nucleotide sequence of the cloned gene was identical to that of TEM-3, except for one substitution (C347A) causing an amino acid exchange at position 49 from leucine to methionine. This TEM-3 variant with both a unique nucleotide and amino acid sequence was designated TEM-139. Transformants producing TEM-3 or TEM-139 expressed identical beta-lactam resistance phenotypes. TEM-139 was the only TEM-type ESBL detected in the surveyed hospitals (seven centres in three towns). TEM-139 is a natural variant of TEM-3 with an amino acid exchange without informational content, detectable only by molecular procedures, e.g. a nucleotide-specific polymerase chain reaction.
authors: MICOER Institute, Hesseloherstrasse 4, 80802 Munich, Germany.
authors: Ines
authors: I
authors: Schneider
authors: Rumyana
authors: R
authors: Markovska
authors: Emma
authors: E
authors: Keuleyan
authors: Mariya
authors: M
authors: Sredkova
authors: Katia
authors: K
authors: Rachkova
authors: Ivan
authors: I
authors: Mitov
authors: Adolf
authors: A
authors: Bauernfeind
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001483:Base Sequence; D002031:Bulgaria / Q000453:epidemiology; D016971:Citrobacter freundii / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D003001:Cloning, Molecular; D004755:Enterobacteriaceae / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D005798:Genes, Bacterial; D014644:Genetic Variation; D006801:Humans; D041121:Klebsiella oxytoca / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D015995:Prevalence; D012189:Retrospective Studies; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: C055184:beta-lactamase TEM-3; D001618:beta-Lactamases