title: Serological evaluation of leptospirosis in Hyderabad Andhra Pradesh: a retrospective hospital-based study.
abstract: OBJECTIVE
  Leptospirosis is a zoonotic disease with humans getting the infection either from rodent hosts or from domestic animals. Urine contaminated environment is the common source of infection. This is an under-reported disease in Andhra Pradesh. We report a retrospective hospital-based study on 55 patients with suspected leptospirosis.
  
  
  METHODS
  A total of 55 serum samples were collected from patients with suspected leptospirosis and subjected to serological testing by LeptoTek Dri-dot, microscopic agglutination test (MAT) and IgM enzyme-linked immunosorbent assay (ELISA). Identification of the predominant infecting serotype was done using a panel of 12 serovars.
  
  
  RESULTS
  MAT analysis of all the 55 samples identified all cases to be positive. The predominant serogroup was Icterohaemorrhagiae (68%) followed by Australis (22%), Autumnalis (8%) and Javanica (2%). LeptoTek Dri-dot showed a sensitivity of 96% as compared to MAT. IgM ELISA done on 32 samples showed a sensitivity of 86.7% compared to MAT.
  
  
  CONCLUSIONS
  MAT helped to identify Icterohemorrhagiae as the predominant serovar in this study. Despite the small number of samples analyzed, the data obtained establishes a need for a prospective study in this region.
authors: Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India.
authors: S
authors: S
authors: Velineni
authors: S
authors: S
authors: Asuthkar
authors: P
authors: P
authors: Umabala
authors: V
authors: V
authors: Lakshmi
authors: M
authors: M
authors: Sritharan
mesh_terms: D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D006386:Hemagglutination Tests; D006761:Hospitals; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology; D007194:India; D007919:Leptospira / Q000254:growth & development / Q000276:immunology; D007922:Leptospirosis / Q000097:blood* / Q000175:diagnosis / Q000276:immunology; D012189:Retrospective Studies; D012698:Serologic Tests
chemical_list: D007075:Immunoglobulin M