Implication of mycelium-associated laccase from Irpex lacteus in the decolorization of synthetic dyes.
The white rot fungus Irpex lacteus is able to decolorize such synthetic dyes as Reactive Orange 16 and Remazol Brilliant Blue R. Here, we demonstrate that this type of dye decolorization is mainly related to a laccase-like enzyme activity associated with fungal mycelium. In its bound form, the enzyme detected showed a pH optimum of 3.0 for the oxidation of ABTS, DMP and guaiacol, and a pH of 7.0 for syringaldazine. The highest enzymatic activity was obtained with ABTS as substrate. Enzyme activity was fully inhibited with 50mM NaN(3). Depending on the chemical structure of dyes, redox mediators had a positive effect on the dye decolorization by fungal mycelium. Enzyme isolated from fungal mycelium was able to decolorize synthetic dyes in vitro.
Svobodová
K
K
Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague, Czech Republic. ksvobod@biomed.cas.cz
Majcherczyk
A
A
Novotný
C
C
Kües
U
U
D000880:Anthraquinones / Q000378:metabolism; D001391:Azo Compounds / Q000378:metabolism; D001487:Basidiomycota / Q000187:drug effects / Q000201:enzymology*; D004396:Coloring Agents / Q000378:metabolism*; D003470:Culture Media; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D042845:Laccase / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D025282:Mycelium / Q000187:drug effects / Q000201:enzymology*; D010084:Oxidation-Reduction / Q000187:drug effects; D013379:Substrate Specificity / Q000187:drug effects; D013696:Temperature
D000880:Anthraquinones; D001391:Azo Compounds; D004396:Coloring Agents; D003470:Culture Media; D004791:Enzyme Inhibitors; C483723:reactive orange 16; D042845:Laccase; C024758:Remazol Brilliant Blue R