Photochemical scar ablation in chronically contused spinal cord of rat.
Glial scar represents a physical and molecular barrier to axonal regeneration and has become an important target for regeneration research in chronic spinal cord injury. Although many methods have been proven useful for the prevention of scar formation in an acute injury model, to date no effective method has been described to remove an existing glial scar in a chronic injury. The chronic lesion possesses an irregular shaped scar that lines the entire perimeter of the cavity. In the present study, we used rose bengal, a molecule commonly used for biological staining, injected into the cavity at the injury site of Long-Evans rat spinal cord (5 weeks after 25-mm contusion injury). Visible light was used to illuminate the injury site. Histological observation illustrates that at least partial glial scar tissue is ablated by rose bengal/illumination. The lack of glial fibrillary acidic protein (GFAP) immunoreactivity at the glial scar coupled with the reduction of GFAP density surrounding spared tissue suggests that this photochemical scar ablation preferentially kills astrocytes at the scar tissue but also reacts, to a lesser degree, in the spared tissue. There is an observed reduction of Basso, Beattie, and Bresnahan (BBB) scale scores after scar ablation, but it is not statistically significant from stabilized behavioral scoring prior to the scar ablation treatment. Our findings indicate that the rose bengal/illumination is feasible for ablation of the glial scar which surrounds an irregular lesion cavity in shape. The scar ablation might provide a permissive environment for the regenerating axons when enriched by cellular or drug therapy.
Zhang
Shuxin
S
Spinal Cord Society Research Center, Fort Collins, Colorado 80526, USA. szhang@scs.cbeyond.com
Kluge
Bridget
B
Huang
Fengfa
F
Nordstrom
Tuija
T
Doolen
Suzanne
S
Gross
Mica
M
Sarmiere
Patrick
P
Holmberg
Eric G
EG
D000818:Animals; D002908:Chronic Disease; D002921:Cicatrix / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism; D005260:Female; D005456:Fluorescent Dyes / Q000627:therapeutic use*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D009457:Neuroglia / Q000502:physiology*; D010778:Photochemotherapy / Q000379:methods*; D051381:Rats; D020318:Rats, Long-Evans; D012395:Rose Bengal / Q000627:therapeutic use*; D013119:Spinal Cord Injuries / Q000150:complications* / Q000378:metabolism / Q000473:pathology; D013904:Thoracic Vertebrae
D005456:Fluorescent Dyes; D005904:Glial Fibrillary Acidic Protein; D012395:Rose Bengal