title: Transformation of human mesenchymal stem cells increases their dependency on oxidative phosphorylation for energy production.
abstract: An increased dependency on glycolysis for ATP production is considered to be a hallmark of tumor cells. Whether this increase in glycolytic activity is due mainly to inherent metabolic alterations or to the hypoxic microenvironment remains controversial. Here we have transformed human adult mesenchymal stem cells (MSC) using genetic alterations as described for differentiated cells. Our data suggest that MSC require disruption of the same pathways as have been shown for differentiated cells to confer a fully transformed phenotype. Furthermore, we found that MSC are more glycolytic than primary human fibroblasts and, in contrast to differentiated cells, do not depend on increased aerobic glycolysis for ATP production during transformation. These data indicate that aerobic glycolysis (the Warburg effect) is not an intrinsic component of the transformation of adult stem cells, and that oncogenic adaptation to bioenergetic requirements, in some circumstances, may also rely on increases in oxidative phosphorylation. We did find, however, a reversible increase in the transcription of glycolytic enzymes in tumors generated by transformed MSC, indicating this is a secondary phenomenon resulting from adaptation of the tumor to its microenvironment.
authors: Cancer Research U.K. Viral Oncology Group, University College London, London WC 1E 6BT, UK.
authors: Juan M
authors: JM
authors: Funes
authors: Marisol
authors: M
authors: Quintero
authors: Stephen
authors: S
authors: Henderson
authors: Dolores
authors: D
authors: Martinez
authors: Uzma
authors: U
authors: Qureshi
authors: Claire
authors: C
authors: Westwood
authors: Mark O
authors: MO
authors: Clements
authors: Dimitra
authors: D
authors: Bourboulia
authors: R Barbara
authors: RB
authors: Pedley
authors: Salvador
authors: S
authors: Moncada
authors: Chris
authors: C
authors: Boshoff
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000328:Adult; D015153:Blotting, Western; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism*; D004734:Energy Metabolism / Q000502:physiology*; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D005947:Glucose / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006019:Glycolysis / Q000502:physiology; D006801:Humans; D016130:Immunophenotyping; D019344:Lactic Acid / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000502:physiology*; D009249:NADP / Q000378:metabolism; D010085:Oxidative Phosphorylation
chemical_list: D019344:Lactic Acid; D009249:NADP; D000255:Adenosine Triphosphate; D005954:Glucosephosphate Dehydrogenase; D005947:Glucose