title: Comparison of reactive oxygen scavenging systems between a cetacean (DKN1) and a porcine renal epithelial cell line (LLC-PK1).
abstract: A transformed renal epithelial cell line, (DKN(1)), from an Atlantic Bottlenose Dolphin, Tursiops truncatus was established in this laboratory and has been used for in vitro genomic analysis and initial toxicological evaluations of dolphin cells. Studies were initiated to compare maintenance of normal antioxidant mechanisms in DKN(1) with similar mechanisms in cells of a pig kidney line, LLC-PK(1). Levels of catalase, glutathione peroxidase, and of reduced glutathione in these dolphin cells were significantly lower than in the porcine cells. Both cell lines were then challenged with hydrogen peroxide at 0.01, 0.1, and 1.0 mM concentrations. The dolphin cells exhibited increased cytotoxicity with a concurrent increase in apoptosis at lower concentrations (0.1 mM) than those required to initiate cytotoxicity in the porcine cells (1.0 mM). Taken together, these results would indicate that the dolphin cells are more susceptible to the damaging effects of certain reactive oxygen species than their terrestrial counterparts.
authors: Department of Veterinary Integrative Biosciences, College of Veterinary Medicine, Texas A&M University, College Station, TX 77843, USA. MPine@cvm.tamu.edu
authors: Michelle D
authors: MD
authors: Pine
authors: Kimberly
authors: K
authors: Greer
authors: David
authors: D
authors: Busbee
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D050681:Bottle-Nosed Dolphin / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D016166:Free Radical Scavengers / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000008:administration & dosage / Q000494:pharmacology; D007668:Kidney / Q000166:cytology* / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013552:Swine
chemical_list: D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D002374:Catalase; D005979:Glutathione Peroxidase; D005978:Glutathione