title: Structural basis for the recognition of blood group trisaccharides by norovirus.
abstract: Noroviruses are one of the major causes of nonbacterial gastroenteritis epidemics in humans. Recent studies on norovirus receptors show that different noroviruses recognize different human histo-blood group antigens (HBGAs), and eight receptor binding patterns of noroviruses have been identified. The P domain of the norovirus capsids is directly involved in this recognition. To determine the precise locations and receptor binding modes of HBGA carbohydrates on the viral capsids, a recombinant P protein of a GII-4 strain norovirus, VA387, was cocrystallized with synthetic type A or B trisaccharides. Based on complex crystal structures observed at a 2.0-A resolution, we demonstrated that the receptor binding site lies at the outermost end of the P domain and forms an extensive hydrogen-bonding network with the saccharide ligand. The A and B trisaccharides display similar binding modes, and the common fucose ring plays a key role in this interaction. The extensive interface between the two protomers in a P dimer also plays a crucial role in the formation of the receptor binding interface.
authors: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China.
authors: Sheng
authors: S
authors: Cao
authors: Zhiyong
authors: Z
authors: Lou
authors: Ming
authors: M
authors: Tan
authors: Yutao
authors: Y
authors: Chen
authors: Yijin
authors: Y
authors: Liu
authors: Zhushan
authors: Z
authors: Zhang
authors: Xuejun C
authors: XC
authors: Zhang
authors: Xi
authors: X
authors: Jiang
authors: Xuemei
authors: X
authors: Li
authors: Zihe
authors: Z
authors: Rao
mesh_terms: D000017:ABO Blood-Group System / Q000737:chemistry* / Q000378:metabolism*; D000595:Amino Acid Sequence; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D008969:Molecular Sequence Data; D029322:Norovirus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism*; D025942:Oligosaccharides, Branched-Chain; D017434:Protein Structure, Tertiary; D011991:Receptors, Virus / Q000737:chemistry / Q000378:metabolism; D014312:Trisaccharides / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D000017:ABO Blood-Group System; D036022:Capsid Proteins; D009844:Oligosaccharides; D025942:Oligosaccharides, Branched-Chain; D011991:Receptors, Virus; D014312:Trisaccharides; C101335:blood group A trisaccharide; C406478:blood group B trisaccharide