[Structure of carbohydrate chains of fucolectin from the bark of golden rain shrub Laburnum anagyroides].
A reductive LiBH4-ButOH cleavage of N-glycosylamide carbohydrate-peptide bond allowed splitting off of oligosaccharide chains of the fucolectin, the bark agglutinin from the shrub golden rain Laburnum anagyroides (LABA). Four N-glycans were isolated by HPLC, and their structures were elucidated by monosaccharide analysis and 1H NMR (500 MHz) spectroscopy: Man2Fuc1XyllGlcNAc2 (M2FX), Man3XyllGlcNAc2 (M3X), Man3FuclXyllGlcNAc2 (M3FX), and Man3XyllFucIGlcNAc3 (NM3FX). All the N-glycans contain D-xylose and three of them, L-fucose; they were found to be in a 1 : 8 : 1 : 3 ratio.
Piskarev
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Iamskov
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D002240:Carbohydrate Sequence; D031296:Laburnum / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification
D037102:Lectins; D009844:Oligosaccharides; D011134:Polysaccharides; C027112:fucose-binding lectin