title: Immunoneutralization of endometrial monoclonal nonspecific suppressor factor beta (MNSFbeta) inhibits mouse embryo implantation in vivo.
abstract: Successful embryo implantation and pregnancy in mammals depends on the establishment of immune tolerance between the maternal immune system and fetal cells. Monoclonal nonspecific suppressor factor beta (MNSFbeta), a cytokine produced by suppressor T cells in various tissues, possesses an antigen-nonspecific immune-suppressive function, and may be involved in the regulation of the uterine immune response during embryo implantation. In this study, anti-MNSFbeta IgG administered directly into the uterine lumen, significantly inhibited mouse embryo implantation in a dose-dependent manner in vivo, and this effect was reversed by co-administration of recombinant MNSFbeta. The effects of anti-MNSFbeta IgG on the gene pattern profiles in mouse uterine tissues were examined by cDNA microarray and several changes were confirmed by real-time PCR. Anti-MNSFbeta IgG caused up-regulation (> or = 2-fold) of 71 known genes and 17 unknown genes, and decreased expression (> or = 2-fold) of 74 known genes and 43 unknown genes, including several genes previously associated with embryo implantation or fetal development. Most of the known genes are involved in immune regulation, cell cycle/proliferation, cell differentiation/apoptosis, and lipid/glucose metabolism. These results demonstrate that MNSFbeta plays critical roles during the early pregnancy via multiple pathways.
authors: Key Laboratory of Contraceptives and Devices of National Population and Family Planning Committee, Shanghai Institute of Planned Parenthood Research, Shanghai, China.
authors: Jian
authors: J
authors: Wang
authors: Zhe Ping
authors: ZP
authors: Huang
authors: Gui Ying
authors: GY
authors: Nie
authors: Lois A
authors: LA
authors: Salamonsen
authors: Qing Xiang
authors: QX
authors: Shen
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000494:pharmacology*; D000917:Antibody Formation / Q000187:drug effects; D010064:Embryo Implantation / Q000187:drug effects* / Q000235:genetics; D004717:Endometrium / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D007074:Immunoglobulin G / Q000276:immunology / Q000494:pharmacology*; D007313:Insecta / Q000166:cytology; D008070:Lipopolysaccharides / Q000276:immunology; D051379:Mice; D008813:Mice, Inbred ICR; D020411:Oligonucleotide Array Sequence Analysis; D011247:Pregnancy; D011817:Rabbits; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013491:Suppressor Factors, Immunologic / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000494:pharmacology
chemical_list: D000906:Antibodies; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D013491:Suppressor Factors, Immunologic; C072169:monoclonal nonspecific suppressor factor