LC-MS characterization of efficacy substances in serum of experimental animals treated with Sophora flavescens extracts.
Anti-DHBV (duck hepatitis B virus) activity was found in the aqueous extracts of Sophora flavescens Ait. in vivo. Liquid chromatography/electrospray ionization ion trap mass spectrometry was applied to characterize the components in duck serum after oral administration of S. flavescens extract. Oxymatrine (1), sophoranol (2), sophoridine (3) and matrine (4) were identified in the serum. Further research on the four compounds was evaluated for their antiviral activity against HBV (hepatitis B virus) in cell culture. The results suggested that oxymatrine, sophoranol and matrine were the efficacy substances for anti-HBV activity in aqueous extracts of S. flavescens Ait.
Ye
Guan
G
Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China.
Zhu
Hai-Yan
HY
Li
Zhi-Xiong
ZX
Ma
Chun-Hui
CH
Fan
Ming-Song
MS
Sun
Zhao-Lin
ZL
Huang
Cheng-Gang
CG
D000470:Alkaloids / Q000097:blood; D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004372:Ducks; D005419:Flavonoids / Q000097:blood; D005562:Formazans / Q000737:chemistry / Q000378:metabolism; D018347:Hepadnaviridae Infections / Q000188:drug therapy* / Q000821:virology; D015611:Hepatitis B Virus, Duck / Q000187:drug effects* / Q000302:isolation & purification / Q000502:physiology; D015394:Molecular Structure; D010936:Plant Extracts / Q000008:administration & dosage*; D011807:Quinolizines / Q000097:blood; D029909:Sophora / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013778:Tetrazolium Salts / Q000737:chemistry / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects*; D000093842:Matrines
D000470:Alkaloids; D000998:Antiviral Agents; D005419:Flavonoids; D005562:Formazans; D010936:Plant Extracts; D011807:Quinolizines; D013778:Tetrazolium Salts; C461819:sophoranone; C079782:MTT formazan; C037573:oxymatrine; D000093842:Matrines