title: Recombination proteins and rescue of arrested replication forks.
abstract: Recombination proteins play crucial roles in the rescue of inactivated replication forks in Escherichia coli. The enzymes that catalyze the repair of DNA double-strand breaks by a classical strand-exchange reaction (RecBCD, RecA) act in two well-characterized fork repair pathways. They repair the DNA double-strand end made when a replication fork runs into a single-strand interruption. They reset the DNA double-strand end generated by replication fork reversal when a component of the replication machinery is inactivated. In addition, recombination proteins also act at replication forks in ways other than the classical strand-exchange reaction. For example, the RuvAB enzyme that catalyzes Holliday junction branch-migration during homologous recombination is also able to catalyze replication fork reversal in certain replication mutants, i.e. to convert certain blocked replication forks into Holliday junctions. Finally, some of the actions of recombination proteins after replication impairment are still unclear, as for example in UV-irradiated cells, where RecFOR and RecA catalyze gap repair but also participate, in a yet undefined way, in "replisome reactivation".
authors: Centre de Génétique Moléculaire, Bâtiment 26, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France. benedicte.michel@cgm.cnrs-gif.fr
authors: Bénédicte
authors: B
authors: Michel
authors: Hasna
authors: H
authors: Boubakri
authors: Zeynep
authors: Z
authors: Baharoglu
authors: Marie
authors: M
authors: LeMasson
authors: Roxane
authors: R
authors: Lestini
mesh_terms: D000818:Animals; D004265:DNA Helicases / Q000235:genetics*; D004261:DNA Replication; D045565:Holliday Junction Resolvases / Q000235:genetics*; D006801:Humans; D016296:Mutagenesis; D011938:Rec A Recombinases / Q000235:genetics*; D011995:Recombination, Genetic
chemical_list: D011938:Rec A Recombinases; D045565:Holliday Junction Resolvases; D004265:DNA Helicases