Rapid discrimination between Candida albicans and Candida dubliniensis by using real-time polymerase chain reaction.
Several phenotypic methods have been used for the differentiation of Candida albicans and Candida dubliniensis, but molecular investigations are considered most reliable in their diagnostic value. Here, we suggest a rapid real-time polymerase chain reaction assay where the discrimination was achieved through melting point analysis with the help of the nonspecific fluorescent dye SybrGreen.
Somogyvari
Ferenc
F
Institute of Clinical Microbiology, Faculty of Medicine, University of Szeged, Szeged 6725, Hungary. fsoma99@hotmail.com
Doczi
Ilona
I
Serly
Julianna
J
Ahmad
Suhail
S
Nagy
Elizabeth
E
D052160:Benzothiazoles; D002175:Candida / Q000145:classification* / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D003959:Diamines; D009930:Organic Chemicals / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D011804:Quinolines
D052160:Benzothiazoles; D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer; D003959:Diamines; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I