Fribrinolysis kinetics and its application.
A new, convenient, and rapid method for kinetic measurement of human fibrinolysis was established. The alteration of absorbance (A) in the process of blood coagulation and lyses was automatically scanned and recorded using a UV2000 spectrophotometer connected to a computer. The parameters of human fibrinolysis kinetics were established. Urokinase at 20 U/mL was the optimal concentration used. There was significant difference in fibrinolysis kinetics and plasma plasminogen concentration between 22 normal subjects and 27 patients with acute myeloblastic leukemia (P<0.05 and <0.01 respectively). The coefficience of variation was (5.24+/-1.51)%. This method could also be used to measure the plasma fibrinogen concentration at the same time. It was concluded that this method was stable and was capable of providing dynamic, direct experimental data and multiparemeters for clinicians. It was also valuable in evaluating the anti-and pro-fibrinolytic capcity of patients' plasmas, allowing for monitoring of therapy, choice of drugs and adjustment of drug concentrations.
Wei
Wenning
W
Institution of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. wenningwei@yahoo.com.cn
Yang
Rui
R
Guo
Tao
T
Yang
Yan
Y
Hu
Yu
Y
D000293:Adolescent; D000328:Adult; D001777:Blood Coagulation / Q000187:drug effects; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D002985:Clinical Protocols; D003435:Crotalid Venoms / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology; D016903:Drug Monitoring; D005260:Female; D005340:Fibrinogen / Q000032:analysis / Q000378:metabolism; D005342:Fibrinolysis / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D015470:Leukemia, Myeloid, Acute / Q000097:blood*; D008297:Male; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000097:blood / Q000473:pathology; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013917:Thrombin / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology; D013997:Time Factors; D014162:Transfection; D014568:Urokinase-Type Plasminogen Activator / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology
D003435:Crotalid Venoms; D005340:Fibrinogen; D013917:Thrombin; C508893:acutobin, Deinagkistrodon acutus; D014568:Urokinase-Type Plasminogen Activator