title: Anion channel blockers attenuate delayed neuronal cell death induced by transient forebrain ischemia.
abstract: Chloride efflux is known to be involved in the progression of apoptosis in various cell types. We have recently shown that the volume-sensitive outwardly rectifying (VSOR) anion channel serves as the pathway for apoptotic chloride efflux in some cells. In the present study, we tested the neuroprotective effects of drugs that can block the VSOR anion channel, on delayed neuronal death (DND) induced by transient forebrain ischemia. The functional expression of the VSOR anion channel was first examined in hippocampal neurons in both primary culture and hippocampal slice preparations, by the whole-cell patch-clamp technique. We then tested the channel's sensitivity to an anion channel blocker, 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS), and a tyrosine kinase blocker, genistein. By histological examinations and cytochrome c release assessments, the protective effects of these drugs on the DND of hippocampal CA1 neurons in mice subjected to transient ischemia were examined. Drugs were administered via the jugular vein prior to ischemic treatment and into the peritoneal cavity after reperfusion. Hippocampal neurons were found to express the volume-sensitive Cl(-) channel, which exhibits outward rectification and is sensitive to DIDS and genistein. Administration of DIDS or genistein reduced cytochrome c release and the number of damaged neurons in the CA1 region after transient forebrain ischemia. This fact suggests that the DND induction mechanism involves the activity of the VSOR anion channel and that this channel may provide a therapeutic target for the treatment of stroke.
authors: Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki, Japan.
authors: Hana
authors: H
authors: Inoue
authors: Hirokazu
authors: H
authors: Ohtaki
authors: Tomoya
authors: T
authors: Nakamachi
authors: Seiji
authors: S
authors: Shioda
authors: Yasunobu
authors: Y
authors: Okada
mesh_terms: D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology; D000704:Analysis of Variance; D000818:Animals; D016923:Cell Death / Q000187:drug effects / Q000502:physiology*; D048429:Cell Size; D018118:Chloride Channels / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002712:Chlorides / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019833:Genistein / Q000494:pharmacology; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D066298:In Vitro Techniques; D002546:Ischemic Attack, Transient / Q000378:metabolism* / Q000473:pathology; D007529:Isoflavones / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018408:Patch-Clamp Techniques; D016548:Prosencephalon / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors
chemical_list: D018118:Chloride Channels; D002712:Chlorides; D004791:Enzyme Inhibitors; D007529:Isoflavones; D018696:Neuroprotective Agents; C004742:daidzein; D019833:Genistein; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid