title: Genetic structure of the malaria vector Anopheles superpictus in Iran using mitochondrial cytochrome oxidase (COI and COII) and morphologic markers: a new species complex?
abstract: Anopheles superpictus has been implicated as the most widespread malaria vector in Iran. We collected adult specimens from eight provinces across the country and subjected them to a morphological investigation as well as molecular analysis of mitochondrial DNA COI-COII region, using PCR-RFLP and analysis of DNA sequence alignment for 708bp of the COI locus. Two distinct morphological forms (A and B) of the species were found sympatric in all areas of study. PCR-RFLP using AluI separated the specimens into at least three genotypes (X, Y, and Z), and alignment of DNA sequences revealed a 12.3% variation in the COI region between the genotypes. However, the sequence variation does not correspond to the morphological forms. Our observations suggest that A. superpictus in Iran is likely a group species. However, further ecological, molecular, cytological, and epidemiological studies are necessary to clear the status of the taxon and the potential role of each putative species in the transmission of malaria.
authors: Department of Medical Entomology, School of Public Health & Institute of Health Research, Medical Sciences/University of Tehran, Tehran, Islamic Republic of Iran. oshaghima@yahoo.com
authors: M A
authors: MA
authors: Oshaghi
authors: Kh
authors: Kh
authors: Shemshad
authors: M R
authors: MR
authors: Yaghobi-Ershadi
authors: M
authors: M
authors: Pedram
authors: H
authors: H
authors: Vatandoost
authors: M R
authors: MR
authors: Abaie
authors: K
authors: K
authors: Akbarzadeh
authors: F
authors: F
authors: Mohtarami
mesh_terms: D000818:Animals; D000852:Anopheles / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D004199:Disease Vectors; D003576:Electron Transport Complex IV / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007492:Iran; D008288:Malaria / Q000635:transmission; D008928:Mitochondria / Q000235:genetics*; D008967:Molecular Biology / Q000379:methods*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D003576:Electron Transport Complex IV