title: Immuno-scanning electron microscopy of collagen types I and III in human vocal fold lamina propria.
abstract: OBJECTIVE
  This study was undertaken to identify the types of collagen fibrils in the extracellular matrix of the human vocal fold lamina propria.
  
  
  METHODS
  Human vocal folds were obtained from 3 autopsy cases less than 65 years of age. The vocal fold specimens were labeled by primary antibodies of anti-type I and anti-type III collagens, and then by secondary antibody conjugated with 15 nm colloidal gold. The specimens were observed with a scanning electron microscope. Secondary electron imaging and backscatter electron imaging of high-resolution field emission scanning electron microscopy were used to detect gold particles indicating immunolabeling.
  
  
  RESULTS
  Type III collagen-labeling gold particles were abundant on the fibrils constructing collagenous fibers, whereas type I collagen-labeling gold particles were sparsely present on fibrils in collagenous fibers. A few reticular fibers were labeled by both collagen type I and collagen type III.
  
  
  CONCLUSIONS
  The results suggest that collagen type I coexists with collagen type III in fibrils of both collagenous fibers and reticular fibers.
authors: Division of Otolaryngology-Head and Neck Surgery, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors: Tomoko
authors: T
authors: Tateya
authors: Ichiro
authors: I
authors: Tateya
authors: Diane M
authors: DM
authors: Bless
mesh_terms: D000328:Adult; D002102:Cadaver; D024042:Collagen Type I / Q000648:ultrastructure*; D024061:Collagen Type III / Q000648:ultrastructure*; D005260:Female; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D016253:Microscopy, Immunoelectron; D008875:Middle Aged; D009092:Mucous Membrane / Q000648:ultrastructure; D012016:Reference Values; D014827:Vocal Cords / Q000648:ultrastructure*
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III