PPARbeta agonists trigger neuronal differentiation in the human neuroblastoma cell line SH-SY5Y.
Neuroblastomas are pediatric tumors originating from immature neuroblasts in the developing peripheral nervous system. Differentiation therapies could help lowering the high mortality due to rapid tumor progression to advanced stages. Oleic acid has been demonstrated to promote neuronal differentiation in neuronal cultures. Herein we report on the effects of oleic acid and of a specific synthetic PPARbeta agonist on cell growth, expression of differentiation markers and on parameters responsible for the malignancy such as adhesion, migration, invasiveness, BDNF, and TrkB expression of SH-SY5Y neuroblastoma cells. The results obtained demonstrate that many, but not all, oleic acid effects are mediated by PPARbeta and support a role for PPARbeta in neuronal differentiation strongly pointing towards PPAR ligands as new therapeutic strategies against progression and recurrences of neuroblastoma.
Di Loreto
S
S
ITOI, CNR, L'Aquila, Italy.
D'Angelo
B
B
D'Amico
M A
MA
Benedetti
E
E
Cristiano
L
L
Cinque
B
B
Cifone
M G
MG
Cerù
M P
MP
Festuccia
C
C
Cimini
A
A
D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D016501:Neurites / Q000187:drug effects; D009447:Neuroblastoma / Q000473:pathology*; D019301:Oleic Acid / Q000494:pharmacology*; D048068:PPAR-beta / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D010524:Peripheral Nervous System Neoplasms / Q000473:pathology*; D034741:RNA, Small Interfering; D020813:Receptor, trkB / Q000235:genetics; D013844:Thiazoles / Q000494:pharmacology*; D010636:Phenols; D013438:Sulfhydryl Compounds
D019208:Brain-Derived Neurotrophic Factor; D019301:Oleic Acid; D048068:PPAR-beta; D034741:RNA, Small Interfering; D020813:Receptor, trkB; D013844:Thiazoles; C479979:(4-(((2-(3-fluoro-4-(trifluoromethyl)phenyl)-4-methyl-1,3-thiazol-5-yl)methyl)sulfanyl)-2-methylphenoxy)acetic acid; D010636:Phenols; D013438:Sulfhydryl Compounds