A storable encapsulated bilayer chip containing a single protein nanopore.
A robust, portable chip containing a single protein nanopore would be a significant development in the practical application of stochastic sensing technology. Here, we describe a chip in which a single alpha-hemolysin (alphaHL) pore in a planar phospholipid bilayer is sandwiched between two layers of agarose gel. These encapsulated nanopore chips remain functional after storage for weeks. The detection of the second messenger inositol 1,4,5-trisphosphate (IP3) was demonstrated with a chip containing a genetically engineered alphaHL pore as the sensor element.
Kang
Xiao-feng
XF
Department of Molecular and Cellular Medicine, The Texas A&M University System Health Science Center, College Station, TX 77843-1114, USA.
Cheley
Stephen
S
Rice-Ficht
Allison C
AC
Bayley
Hagan
H
D049329:Nanostructures; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods*; D011506:Proteins / Q000032:analysis*
D011506:Proteins