title: Characterization of two types of osteoclasts from human peripheral blood monocytes.
abstract: The two osteoclastogenesis pathways, receptor activator nuclear factor (NF)-kappaB ligand (RANKL)-mediated and fusion regulatory protein-1 (FRP-1)-mediated osteoclastogenesis, have recently been reported. There were significant differences in differentiation and activation mechanisms between the two pathways. When monocytes were cultured with FRP-1 without adding M-CSF, essential for the RANKL system, TRAP-positive polykaryocyte formation occurred. FRP-1-mediated osteoclasts formed larger pits on mineralized calcium phosphate plates than RANKL+M-CSF-mediated osteoclasts did. Lacunae on dentin surfaces induced by FRP-1-mediated osteoclasts were inclined to be single and isolated. However, osteoclasts induced by RANKL+M-CSF made many connected pits on dentin surfaces as if they crawled on there. Interestingly, FRP-1 osteoclastogenesis was enhanced by M-CSF/IL-1alpha, while chemotactic behavior to the dentin slices was not effected. There were differences in pH and concentration of HCO3- at culture endpoint and in adherent feature to dentin surfaces. Our findings indicate there are two types of osteoclasts with distinct properties.
authors: Department of Microbiology, Mie University Graduate School of Medicine, Japan. yuasa911@do.medic.mie-u.ac.jp
authors: Kimitaka
authors: K
authors: Yuasa
authors: Kouki
authors: K
authors: Mori
authors: Hitoshi
authors: H
authors: Ishikawa
authors: Akihiro
authors: A
authors: Sudo
authors: Atsumasa
authors: A
authors: Uchida
authors: Yasuhiko
authors: Y
authors: Ito
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D001862:Bone Resorption / Q000473:pathology; D024402:Carbonic Anhydrase II / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D027261:Fusion Regulatory Protein-1 / Q000276:immunology*; D015726:Giant Cells / Q000166:cytology; D006801:Humans; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D008854:Microscopy, Electron; D009000:Monocytes / Q000166:cytology / Q000187:drug effects*; D010010:Osteoclasts / Q000166:cytology*; D053245:RANK Ligand / Q000494:pharmacology*
chemical_list: D000911:Antibodies, Monoclonal; D027261:Fusion Regulatory Protein-1; D053245:RANK Ligand; D016173:Macrophage Colony-Stimulating Factor; D024402:Carbonic Anhydrase II