Transcriptional inhibition by a non-histone protein from low mobility group in homologous and heterologous in vitro systems.
An acidic non-histone chromosomal protein of low mobility group (LMG) with pI of 5-5.5 and molecular weight of 160 kDa (named LMG160), has been isolated from rat liver nuclei and its inhibitory effect on transcription of DNA in vitro has been detected in a heterologous prokaryotic in vitro transcription system (T7 RNA polymerase) and also confirmed in the homologous cell-free system of rat liver nuclear extract. The results indicate that in the presence of non-histone protein LMG160, in vitro RNA synthesis represses in the both systems, which can suggest a functional role for this non-histone chromosomal protein.
Shahhoseini
Maryam
M
Institute of Biochemistry and Biophysics, Department of Biochemistry, University of Tehran, Tehran, P.O. Box 13145-1384, Iran.
Rabbani-Chadegani
Azra
A
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000302:isolation & purification / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D008110:Liver Extracts / Q000378:metabolism; D051381:Rats; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000378:metabolism
D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D008110:Liver Extracts; D014764:Viral Proteins; C476927:low mobility group protein 160, rat; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases