Development of new-generation HU-PBMC-NOD/SCID mice to study human islet alloreactivity.
The use of "humanized" mice represents an appealing translational model for studies of the pathogenesis of immune-mediated diseases and for the evaluation of potential therapeutics. The utility of humanized mice depends on their ability to model the human immune system with high fidelity, and, in this respect, previous models have fallen short. The recently developed NOD-scid Il2rgamma(null) mouse, however, exhibits greatly enhanced ability to support the engraftment of human peripheral blood mononuclear cells. Herein, we describe the challenges of recapitulating human immunity in humanized mice and features of NOD-scid Il2rgamma(null) mice that help overcome them.
King
Marie
M
Diabetes Division, University of Massachusetts Medical School, 373 Plantation Street, Suite 218, Worcester, MA 01605, USA.
Pearson
Todd
T
Shultz
Leonard D
LD
Leif
Jean
J
Bottino
Rita
R
Trucco
Massimo
M
Atkinson
Mark
M
Wasserfall
Clive
C
Herold
Kevan
K
Mordes
John P
JP
Rossini
Aldo A
AA
Greiner
Dale L
DL
D000818:Animals; D003433:Crosses, Genetic; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D004195:Disease Models, Animal; D006801:Humans; D007515:Islets of Langerhans / Q000276:immunology*; D007519:Isoantigens / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016688:Mice, Inbred NOD / Q000235:genetics*; D016513:Mice, SCID / Q000235:genetics*
D007519:Isoantigens