Study of blood porphyrin spectral profile for diagnosis of tumor progression.
Renal cell carcinoma (RCC) accounts for approximately 3% of new cancer incidence and mortality in the United States. Unfortunately many RCC masses remain asymptomatic and nonpalpable until they are advanced. Diagnosis and localization of early carcinoma play an important role in the prevention and curative treatment of RCC. The autofluorescence of blood porphyrin of healthy and tumor induced in male SCID mice was analyzed using fluorescence and excitation spectroscopy. A significant contrast between normal and tumor blood could be established. Blood porphyrin fluorophore showed enhanced fluorescence band (around 630 nm) in function of the tumor growth. This indicates that either the autofluorescence intensity of the blood fluorescence may provide a good parameter for the "first approximation" characterization of the tumor stage.
Courrol
Lilia Coronato
LC
Departamento de Ciências Exatas, UNIFESP, Campus Diadema, Brazil. lcourrol@gmail.com
de Oliveira Silva
Flávia Rodrigues
FR
Coutinho
Enia Lúcia
EL
Piccoli
Michelly França
MF
Mansano
Ronaldo Domingues
RD
Vieira Júnior
Nilson Dias
ND
Schor
Nestor
N
Bellini
Maria Helena
MH
D000818:Animals; D002292:Carcinoma, Renal Cell / Q000097:blood / Q000175:diagnosis / Q000473:pathology; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D018450:Disease Progression; D006801:Humans; D008297:Male; D051379:Mice; D016513:Mice, SCID; D009367:Neoplasm Staging / Q000379:methods; D009369:Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000473:pathology; D011166:Porphyrins / Q000097:blood* / Q000737:chemistry; D011524:Protoporphyrins / Q000097:blood / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D011166:Porphyrins; D011524:Protoporphyrins; C028025:protoporphyrin IX