title: Examination of basement membrane components associated with the bovine seminiferous tubule basal lamina.
abstract: Immunohistology has been used to examine the distribution of certain components of the basement membrane (BM) associated with bovine spermatogonial germ cells that are located within the seminiferous tubules. Histology was performed on testis tissue from Brahman cattle (Bos indicus) of three different age groups: pre-pubescent (4-6 months), juvenile (8-10 months) and adult (18-24 months) animals. There were no major changes in the BM composition apparent between these three age groups, except for certain lectin staining. These data suggest that the predominant collagen type IV component may have an alpha3 and alpha4 composition, although other chains, including the alpha5 and alpha6 chains, were also present. Possibly the main laminin type present was laminin 121 (alpha1beta2gamma1), although other variants were also present. Both nidogen-1 and perlecan, which are normal BM components, were also found as part of the seminiferous tubule BM. Interstitial collagens, such as type I, III and VI collagens, were found in the peritubular space, but were not part of the BM itself, although type VI collagen was most visible in the peritubular zone adjacent to the tubules. Examination of the BM with a range of lectins gave strong staining for (glcNAc)(2) entities, weak positive staining for alpha-l-fuc, but little or no staining for alpha-galNAc and (glcNAc)(3) at all ages, whereas staining for alpha-gal, beta-gal(1-->3)galNAc and alpha-man showed developmental changes.
authors: CSIRO Food Futures National Research Flagship, and CSIRO Molecular and Health Technologies, Bayview Avenue, Clayton, Victoria 3169, Australia. veronica.glattauer@csiro.au
authors: Veronica
authors: V
authors: Glattauer
authors: Helen F
authors: HF
authors: Irving-Rodgers
authors: Raymond J
authors: RJ
authors: Rodgers
authors: Sally
authors: S
authors: Stockwell
authors: Alan G
authors: AG
authors: Brownlee
authors: Jerome A
authors: JA
authors: Werkmeister
authors: John A M
authors: JA
authors: Ramshaw
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000737:chemistry* / Q000166:cytology; D002417:Cattle; D024022:Fibrillar Collagens / Q000032:analysis*; D007150:Immunohistochemistry; D007797:Laminin / Q000032:analysis*; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis*; D012671:Seminiferous Tubules / Q000737:chemistry* / Q000166:cytology
chemical_list: D024022:Fibrillar Collagens; D007797:Laminin; D008562:Membrane Glycoproteins