[Correlation between adenylosuccinate lyase (ADSL) gene polymorphism and inosine monophosphate acid (IMP) content in domestic fowl and genetic relationship between red jungle fowl and domestic fowl].
This study investigates single nucleotide polymorphism (SNP) of the adenylosuccinate lyase(ADSL) gene in variety chicken breeds, including Recessive White chickens, Silkies chickens, Baier chickens, Tibetan chickens and two red jungle fowls. Primers for exon 2 in ADSL gene were designed based on the chicken genomic sequence and a SNP(C/T at 3484) was detected by PCR-SSCP and DNA sequencing. Three genotypes within all breeds were found and least square analysis showed that TT genotype birds had a significant higher inosine monophosphate acid (IMP) content than TC (P < 0.01) and CC (P < 0.05) genotype birds, TC genotype birds had a little higher IMP content than CC genotype birds, but the difference was not significant. We proposed this SNP site correlated with IMP content in chickens. A neighbour-joining dendrogram was constructed based on the Nei's genentic distance. The genetic relationship between Chinese red jungle fowl and Tibetan chickens was the nearest, whereas Baier chickens were more closer to Silkies chickens. The Chinese red jungle fowls were relatively closer to the domestic fowls, whereas Thailand red jungle fowls were relatively diverging to the Chinese native breeds. These results supported the theory concerning the independent origins of Chinese native fowl breeds.
Shu
Jing-Ting
JT
College of Animal Science and Technology, Yangzhou University, Yangzhou, China. shujingting@163.com
Bao
Wen-Bin
WB
Zhang
Hong-Xia
HX
Zhang
Xue-Yu
XY
Ji
Cong-Liang
CL
Chen
Guo-Hong
GH
D000264:Adenylosuccinate Lyase / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D001947:Breeding; D002645:Chickens; D005787:Gene Frequency; D007291:Inosine Monophosphate / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D018807:Polymorphism, Single-Stranded Conformational; D011200:Poultry / Q000235:genetics*
D007291:Inosine Monophosphate; D000264:Adenylosuccinate Lyase