title: Cloning of the Arthrobacter sp. FG1 dehalogenase genes and construction of hybrid pathways in Pseudomonas putida strains.
abstract: An Arthrobacter strain, able to utilize 4-chlorobenzoic acid as the sole carbon and energy source, was isolated and characterized. The first step of the catabolic pathway was found to proceed via a hydrolytic dehalogenation that leads to the formation of 4-hydroxybenzoic acid. The dehalogenase encoding genes (fcb) were sequenced and found highly homologous to and organized as those of other 4-chlorobenzoic acid degrading Arthrobacter strains. The fcb genes were cloned and successfully expressed in the heterologous host Pseudomonas putida PaW340 and P. putida KT2442 upper TOL, which acquired the ability to grow on 4-chlorobenzoic acid and 4-chlorotoluene, respectively. The cloned dehalogenase displayed a high specificity for para-substituted haloaromatics with affinity Cl > Br > I >> F, in the order.
authors: Dipartimento di Biologia Strutturale e Funzionale, via Dunant 3, 21100, Varese, Italy.
authors: Francesca
authors: F
authors: Radice
authors: Viviana
authors: V
authors: Orlandi
authors: Valentina
authors: V
authors: Massa
authors: Vanessa
authors: V
authors: Battini
authors: Giovanni
authors: G
authors: Bertoni
authors: Walter
authors: W
authors: Reineke
authors: Paola
authors: P
authors: Barbieri
mesh_terms: D001173:Arthrobacter / Q000201:enzymology* / Q000235:genetics*; D002723:Chlorobenzoates / Q000378:metabolism*; D003001:Cloning, Molecular; D005818:Genetic Engineering; D016958:Pseudomonas putida / Q000201:enzymology / Q000235:genetics*; D014050:Toluene / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D002723:Chlorobenzoates; D014050:Toluene; C040768:4-chlorobenzoic acid; C029049:4-chlorotoluene