Determination of patulin in apple and hawthorn beverages by solid-phase filtration column and liquid chromatography.
The use of solid-phase filtration column (MycoSep 228) and liquid chromatography for determination of patulin in apple and hawthorn beverages was investigated. The method passed a series of validation tests, including separation efficiency, sensitivity, repeatability, validation within a collaborative trial, recoveries, and applicability. Accuracy and precision were very good for determination of patulin in both spiked and naturally contaminated apple/hawthorn beverage samples at levels of 10 microg/L and above, with limits of detection and quantification at 8 and 23 [microg/L, respectively. The applicability of the method was tested by analyzing commercially available domestic apple and hawthorn beverages. Patulin was found in 3 of 52 (5.77%) apple beverages at concentrations ranging from 63.45 to 88.44 [microg/L, and in 6 of 43 (13.95%) hawthorn beverages ranging from 19.8 to 206.88 microg/L. Among these were 3 apple and 4 hawthorn beverage samples with toxin levels that exceeded the Chinese legislative requirement of 50 microg/L in apple- and hawthorn-based products intended for human consumption. Comparable tests revealed that the results obtained by both the new MycoSep-based method and the official AOAC method are not significantly different (t value = 0.003268 < to.05, P > 0.05).
Li
Fengqin
F
Institute for Nutrition and Food Safety, Chinese Centre for Disease Control and Prevention, Beijing 100021, People's Republic of China. lifengqin@yahoo.com.cn
Zhao
Shan
S
Chin
Leejiuan
L
Li
Yuwei
Y
Wu
Duojia
D
Zhao
Xi
X
Han
Chunhui
C
Zhang
Hongyuan
H
Ji
Rong
R
D001628:Beverages / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods; D027825:Crataegus / Q000737:chemistry*; D005374:Filtration; D007202:Indicators and Reagents; D027845:Malus / Q000737:chemistry*; D010365:Patulin / Q000032:analysis / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D007202:Indicators and Reagents; D010365:Patulin