title: Absorption of horseradish peroxidase in Bombyx mori larval midgut.
abstract: Increasing experimental evidence indicates that ingested proteins can in part reach the haemocoel undegraded, but information on the mechanisms involved in protein transport across the insect gut is very limited, in spite of the implications that this may have on the development of novel delivery strategies of insecticide proteins targeting haemocoelic receptors. Here we contribute to this field of study, by focusing on horseradish peroxidase (HRP) transport through Bombyx mori larval midgut, isolated and perfused in vitro. The protein crossed the intestinal barrier in a time-dependent manner and the influx was linearly related to time between 30 and 90 min of incubation. HRP absorption was strongly affected by temperature and inhibition of cell metabolism: protein influx at 4 degrees C was reduced to 27% of that measured at 25 degrees C and was similarly inhibited by the metabolic inhibitor DNP. Transmission electron microscopy analysis of midgut columnar cells exposed to HRP showed the presence of the protein both in vesicular structures inside the cytoplasm and in the space between two adjacent absorptive cells, indicating the occurrence of both a transcellular and a paracellular permeation route. The analysis of HRP influx as a function of increasing protein concentration in the lumen supported this morphological indication. The J(max) relative to the HRP transcellular transport component was 121+/-24 pmol/cm(2)/h and the K(d) of the passage through the paracellular route was 1.9+/-0.3 microl/cm(2)/h. The paracellular electrical resistance decreased in midguts exposed to HRP, indicating that its passage through this pathway was likely due to an alteration exerted on the junctional complex by the protein itself. The role of the cytoskeleton in HRP transport was investigated by assessing the impact of drugs affecting microtubules and actin filaments.
authors: Dipartimento di Biologia, Università degli Studi di Milano, via Celoria 26, 20133 Milan, Italy. morena.casartelli@unimi.it
authors: Morena
authors: M
authors: Casartelli
authors: Paola
authors: P
authors: Corti
authors: Gaia
authors: G
authors: Cermenati
authors: Annalisa
authors: A
authors: Grimaldi
authors: Luisa
authors: L
authors: Fiandra
authors: Nadia
authors: N
authors: Santo
authors: Francesco
authors: F
authors: Pennacchio
authors: Barbara
authors: B
authors: Giordana
mesh_terms: D000818:Animals; D012831:Bombyx / Q000378:metabolism* / Q000648:ultrastructure; D004064:Digestive System / Q000378:metabolism*; D017097:Electric Impedance; D006458:Hemolymph / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000493:pharmacokinetics*; D066298:In Vitro Techniques; D007408:Intestinal Absorption; D007814:Larva; D046529:Microscopy, Electron, Transmission
chemical_list: D006735:Horseradish Peroxidase