Production of dye-decolorizing peroxidase (rDyP) from complex substrates by repeated-batch and fed-batch cultures of recombinant Aspergillus oryzae.
We examined production levels of dye-decolorizing peroxidase (rDyP) by recombinant Aspergillus oryzae using wheat bran and rice bran powders in repeated-batch and fed-batch cultures. Similar average rDyP productivities were observed in repeated-batch cultures using wheat bran powder and rice bran powder. Average rDyP productivities in fed-batch cultures were slightly lower than those in repeated-batch cultures. The rDyP production was affected by the addition of K(2)HPO(4) in the repeated-batch and fed-batch cultures using wheat bran powder. All average rDyP productivities in this study were significantly higher than those for any other peroxidases previously reported.
Shakeri
Mozaffar
M
Chemical Resources Laboratory, Tokyo Institute of Technology, Yokohama, Japan.
Sugano
Yasushi
Y
Shoda
Makoto
M
D001236:Aspergillus oryzae / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D001709:Biotechnology / Q000379:methods*; D018929:Cell Culture Techniques; D004396:Coloring Agents / Q000737:chemistry; D004043:Dietary Fiber / Q000378:metabolism; D005818:Genetic Engineering; D010544:Peroxidases / Q000096:biosynthesis* / Q000737:chemistry; D010710:Phosphates / Q000378:metabolism; D017680:Potassium Compounds / Q000378:metabolism
D004396:Coloring Agents; D004043:Dietary Fiber; D010710:Phosphates; D017680:Potassium Compounds; C013216:potassium phosphate; D010544:Peroxidases