title: In vitro antimicrobial activity of different gutta-percha points and calcium hydroxide pastes.
abstract: The aim of this study was to evaluate the antimicrobial activity of different trademarks and compositions of gutta-percha points and calcium hydroxide pastes used in endodontic therapy. The evaluated material consisted of gutta-percha points containing calcium hydroxide (Roeko), gutta-percha points containing chlorhexidine (Roeko), two conventional gutta-percha points (Endo Points and Roeko) and two calcium hydroxide pastes (Calen and Calen/PMCC). Antimicrobial tests included five species of microorganisms: Escherichia coli (ATCC10538), Staphylococcus epidermidis (ATCC12228), Staphylococcus aureus (ATCC6538), Pseudomonas aeruginosa (ATCC27853), and Micrococcus luteus (ATCC9341). The Agar diffusion method was employed. The plates were kept at room temperature for 2 h for prediffusion and then incubated at 37 degrees C for 24 h. The triphenyltetrazolium chloride gel was added for optimization and the zones of inhibition were measured. Statistical evaluation was carried out using analysis of variance and Tukey Test. The obtained results showed that all microbial species used in the study were inhibited by the gutta-percha points containing chlorhexidine and by the calcium hydroxide pastes (Calen and Calen/PMCC), with similar results (p > 0.05). No antimicrobial activity was observed for the other groups. It was concluded that the gutta-percha points containing chlorhexidine presented antimicrobial activity, whereas the gutta-percha points containing calcium hydroxide did not.
authors: School of Dentistry of Araraquara, State University of São Paulo, Rua Expedicionários do Brazil 2030, Araraquara, SP, Brazil, CEP 14801-360. jutanomaru@uol.com.br
authors: Juliane Maria Guerreiro
authors: JM
authors: Tanomaru
authors: Fernanda Geraldes
authors: FG
authors: Pappen
authors: Mário
authors: M
authors: Tanomaru Filho
authors: Denise Madalena Palomari
authors: DM
authors: Spolidorio
authors: Izabel Yoko
authors: IY
authors: Ito
mesh_terms: D000704:Analysis of Variance; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D002126:Calcium Hydroxide / Q000494:pharmacology*; D002710:Chlorhexidine / Q000494:pharmacology*; D004708:Endodontics; D004926:Escherichia coli / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D006171:Gutta-Percha / Q000592:standards; D016982:Micrococcus luteus / Q000187:drug effects; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D013212:Staphylococcus epidermidis / Q000187:drug effects; D014092:Tooth Root / Q000187:drug effects / Q000382:microbiology
chemical_list: D000891:Anti-Infective Agents, Local; D006171:Gutta-Percha; D002126:Calcium Hydroxide; D002710:Chlorhexidine