Simultaneous production of glucoamylase and acid-stable alpha-amylase using novel submerged culture of Aspergillus kawachii NBRC4308.
We developed a novel submerged culture system of Aspergillus kawachii NBRC4308 using barley whose surface is completely or partly covered with husk. The culture supernatant showed a glucoamylase activity of 150.8 U/ml and an acid-stable alpha-amylase activity of 7.7 U/ml brought about by the maintenance of a low glucose concentration in the culture system.
Shoji
Hiroshi
H
Brewing Research & Development Laboratory, Asahi Breweries, Ltd., 1-21 Midori 1-Chome, Moriya, Ibaraki 302-0106, Japan. hiroshi.shoji@asahibeer.co.jp
Sugimoto
Toshikazu
T
Hosoi
Kenji
K
Shibata
Kazunori
K
Tanabe
Masayuki
M
Kawatsura
Katsuyuki
K
D000143:Acids; D001230:Aspergillus / Q000254:growth & development* / Q000378:metabolism; D018929:Cell Culture Techniques; D004795:Enzyme Stability; D005285:Fermentation; D005087:Glucan 1,4-alpha-Glucosidase / Q000096:biosynthesis*; D005947:Glucose / Q000032:analysis; D001467:Hordeum / Q000737:chemistry; D009172:Mycology / Q000379:methods*; D000516:alpha-Amylases / Q000096:biosynthesis*
D000143:Acids; D000516:alpha-Amylases; D005087:Glucan 1,4-alpha-Glucosidase; D005947:Glucose