Analysis of mycobacterial strains by two-dimensional gel electrophoresis.
This study pertains to analysis of the protein profile of different mycobacterial strains by two-dimensional gel electrophoresis (2DE). The strains were selected as they exhibit different phenotypic behaviour. TCA-acetone precipitated proteins were resolved by 2DE using immobilized pH gradient (IPG) strips. This study demonstrates that 2DE may be used as a tool for characterization of mycobacterial strains. Visual examination of the electrophoretograms was sufficient for characterization. Detailed characterization of specific proteins might lead to development of novel targets, diagnostic probes or sub-unit vaccine(s) against tuberculosis.
Bisht
Deepa
D
Department of Biochemistry, National JALMA Institute for Leprosy and Other Mycobacterial Diseases (ICMR), Tajganj, Agra- 282001, India. deepa_bisht@lycos.com
Singhal
Neelja
N
Sharma
Prashant
P
Venkatesan
K
K
D001426:Bacterial Proteins / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D009163:Mycobacterium bovis / Q000737:chemistry / Q000145:classification*; D020102:Mycobacterium smegmatis / Q000737:chemistry / Q000145:classification*; D009169:Mycobacterium tuberculosis / Q000737:chemistry / Q000145:classification*
D001426:Bacterial Proteins