The complete mitochondrial genome of the Korean soft-shelled turtle Pelodiscus sinensis (Testudines, Trionychidae).
We isolated Korean soft-shelled turtle, Pelodiscus sinensis, mitochondrial DNA by long-polymerase chain reaction (long-PCR) with conserved primers and sequenced this mitochondrial genome (mitogenome) with primer walking using flanking sequences. The P. sinensis mitochondrial DNA has 17,042 bp and its structural organization is conserved compared to those of other reptiles and mammals. To unveil the phylogenetic relationship of the turtles, we used the NJ, MP, and ML analysis methods after inferring those sequences from the mitochondrial 16S rRNA gene. We also compared two P. sinensis variants from Korea and China using the mitochondrial genome. In this study, we report the basic characteristics of the P. sinensis mitochondrial genome, including structural organization and base composition of the rRNAs, tRNAs and protein-coding genes, as well as characteristics of tRNAs. These features are applicable for the study of phylogenetic relationships in turtles.
Jungt
Sang-Oun
SO
Department of Molecular and Environmental Bioscience, Graduate School, Hanyang University, Seoul 133-791, Korea
Lee
Young-Mi
YM
Kartavtsev
Yuri
Y
Park
In-Seok
IS
Kim
Dong Soo
DS
Lee
Jae-Seong
JS
D000818:Animals; D001482:Base Composition; D020029:Base Pairing; D001483:Base Sequence; D002681:China; D016000:Cluster Analysis; D017124:Conserved Sequence / Q000235:genetics; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics*; D023061:Gene Order; D007723:Korea; D016013:Likelihood Functions; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014426:Turtles / Q000235:genetics*
D017931:DNA Primers; D004272:DNA, Mitochondrial