Determination of triadimenol based on the quenching effect on resonance light scattering from the triadimenol-deoxyribonucleic acid-hydrochloric acid system.
Analysis of triadimenol was carried out using deoxyribonucleic acids (DNA) via the resonance light scattering (RLS) technique. After adding triadimenol into aqueous medium of pH 1.72, the RLS of DNA was remarkably quenched. A resonance light scattering peak at 310 nm was found, and the quenched intensity of RLS at this wavelength was proportional to the concentration of triadimenol. The linear range of the calibration curve was approximately 0-3 microg mL-1 with a detection limit (S/N=3) of 0.07 microg mL-1. The triadimenol in samples of water, cucumber and human serum was determined. The results were satisfactory, and the recovery rates were in the range of 96.3-106.0%, 94.8-105.9% and 92.3-100.5%, respectively. The interaction mechanism was also studied.
Du
Fengpei
F
College of Science, China Agricultural University, Beijing, 100094, China.
Luo
Xiaolin
X
Jiang
Guibin
G
Hou
Shicong
S
Liu
Gang
G
Ren
Liping
L
Zhang
Li
L
Huang
Qin
Q
Jie
Nianqin
N
D002138:Calibration; D018553:Cucumis sativus / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D005618:Fresh Water / Q000032:analysis; D005659:Fungicides, Industrial / Q000032:analysis / Q000097:blood; D006801:Humans; D006851:Hydrochloric Acid / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008027:Light; D009994:Osmolar Concentration; D010575:Pesticides / Q000737:chemistry; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet; D014230:Triazoles / Q000032:analysis* / Q000097:blood / Q000737:chemistry
D005659:Fungicides, Industrial; D010575:Pesticides; D014230:Triazoles; C032910:triadimefon; D004247:DNA; C427203:calf thymus DNA; D006851:Hydrochloric Acid