Synthesis, and DNA-binding and DNA-photocleavage properties of multiply charged porphyrins.
Four new cationic porphyrins, compounds 1-4, with five to seven positive charges, were synthesized, characterized, and investigated for their binding properties towards calf-thymus DNA (CT-DNA). UV/VIS and fluorescence-titration data indicated strong binding, the apparent binding constants (K(app); (1.3-10)x10(-6) M) increasing with increasing number of charges, as determined by competitive fluorescence titration using ethidium bromide (EB) as molecular probe. These results were qualitatively confirmed by the observed photocleavage efficiency of the porphyrins towards plasmid pBR322 DNA.
Wang
Kai
K
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
Zhang
Zhi
Z
Wu
Lei
L
Li
Zao-Ying
ZY
D004247:DNA / Q000378:metabolism*; D008027:Light; D010777:Photochemistry; D010957:Plasmids / Q000378:metabolism; D011166:Porphyrins / Q000138:chemical synthesis* / Q000378:metabolism; D011485:Protein Binding / Q000502:physiology
D011166:Porphyrins; D004247:DNA; C427203:calf thymus DNA