Mixed-ligand copper(II)-phenanthroline-dipeptide complexes: synthesis, characterization, and DNA-cleavage properties.
The mixed-ligand complexes [Cu(II)(HisLeu)(phen)](+) (1) and [Cu(II)(HisSer)(phen)](+) (2; phen=1,10-phenanthroline) were synthesized and characterized. The intercalative interaction of the Cu(II) complexes with calf-thymus DNA (CT-DNA) was probed by UV/VIS and fluorescence titration, as well as by thermal-denaturation experiments, and the intrinsic binding constants (K(b)) for the complexes with 1 and 2 were 4.2x10(3) and 4.9x10(3) M(-1), resp. Both complexes were found to be efficient catalysts for the hydrolytic cleavage of plasmid pUC19 DNA, as tested by gel electrophoresis, converting the DNA from the supercoiled to the nicked-circular form at rate constants of 1.32 and 1.40 h(-1) for 1 and 2, resp.
Reddy
Pulimamidi R
PR
Department of Chemistry, Osmania University, Hyderabad-500 007, India. rabi_pr@rediffmail.com
Manjula
Pallerla
P
D000818:Animals; D002417:Cattle; D003300:Copper / Q000737:chemistry* / Q000494:pharmacology; D004247:DNA / Q000138:chemical synthesis / Q000494:pharmacology; D053837:DNA Cleavage / Q000187:drug effects*; D004151:Dipeptides / Q000138:chemical synthesis* / Q000494:pharmacology; D010618:Phenanthrolines / Q000138:chemical synthesis* / Q000494:pharmacology; D011485:Protein Binding / Q000187:drug effects / Q000502:physiology
D004151:Dipeptides; D010618:Phenanthrolines; D003300:Copper; D004247:DNA; C427203:calf thymus DNA