title: Identification of phostensin, a PP1 F-actin cytoskeleton targeting subunit.
abstract: We have identified a novel protein, protein phosphatase 1 F-actin cytoskeleton targeting subunit (phostensin). This protein is encoded by KIAA1949 and was found to associate with protein phosphatase 1 (PP1) in the yeast two-hybrid assay, co-immunoprecipitation, and GST pull-down assay. Northern blot analysis revealed that phostensin mRNA was predominantly distributed in leukocytes and spleen, and phostensin protein was present in crude extracts of human peripheral leukocytes. Immunofluorescence microscopic analysis revealed that the phostensin/PP1 complex was conspicuously localized with the actin cytoskeleton at the cell periphery in Madin-Darby canine kidney (MDCK) epithelial cells. Taken together, our data shows that phostensin targets PP1 to F-actin cytoskeleton. The phostensin/PP1 complex may play a vital role in modulation of actin rearrangements.
authors: Department of Life Science, National Chung Hsing University, Taichung, Taiwan.
authors: Shu-Chen
authors: SC
authors: Kao
authors: Chun-Yu
authors: CY
authors: Chen
authors: Shiu-Lan
authors: SL
authors: Wang
authors: Jaw-Ji
authors: JJ
authors: Yang
authors: Wei-Chuan
authors: WC
authors: Hung
authors: Yi-Chen
authors: YC
authors: Chen
authors: Ning-Sheng
authors: NS
authors: Lai
authors: Hsin-Tzu
authors: HT
authors: Liu
authors: Hsien-lu
authors: HL
authors: Huang
authors: Hong-Chen
authors: HC
authors: Chen
authors: Ta-Hsien
authors: TH
authors: Lin
authors: Hsien-bin
authors: HB
authors: Huang
mesh_terms: D000199:Actins / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D004285:Dogs; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007962:Leukocytes / Q000737:chemistry; D010749:Phosphoprotein Phosphatases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D054645:Protein Phosphatase 1; D020798:Two-Hybrid System Techniques
chemical_list: D000199:Actins; C520010:PPP1R18 protein, human; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1