Isolation of high-purity casticin from Artemisia annua L. by high-speed counter-current chromatography.
Following an initial clean-up step on silica, high-speed counter-current chromatography (HSCCC) was used to purify a flavone, casticin (5,3'-dihydroxy-3,6,7, 4'-tetramethoxyflavone), from an extract of the dried leaves of Artemisia annua L. The two-phase solvent system used was composed of n-hexane-ethyl acetate-methanol-water at an optimized volume ratio of 7:10:7:10 (v/v). HSCCC separation of 226.4 mg of crude sample (containing casticin at 16.5% purity after silica gel clean-up) yielded 36.3 mg of casticin with a purity of over 99% and 96.2% recovery. Identification of the target compound was performed by (1)H NMR, (13)C NMR, two-dimensional NMR, electrospray ionization MS, IR and UV.
Han
Xiao
X
Chinese Key Laboratory on National Reference Materials for Natural Products, Beijing UE Biotech Co. Ltd., Beijing 100035, China. ssisyhan@yahoo.com.cn
Ma
Xiaofeng
X
Zhang
Tianyou
T
Zhang
Yabin
Y
Liu
Qinghui
Q
Ito
Yoichiro
Y
D031172:Artemisia annua / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification*; D015394:Molecular Structure
D005419:Flavonoids; C054133:casticin