Cyperus rotundus extract inhibits acetylcholinesterase activity from animal and plants as well as inhibits germination and seedling growth in wheat and tomato.
Cyperus rotundus (nutgrass) is the world's worst invasive weed through tubers. Its success in dominating natural habitats depends on its ability to prevent herbivory, and to kill or suppress other plants growing in its vicinity. The present study was done to investigate whether chemicals in nutgrass target neuronal and non-neuronal acetylcholinesterases to affect surrounding animals and plants respectively. Methanolic extract of tubers of nutgrass strongly inhibited activity of AChE from electric eel, wheat and tomato. It also inhibited seed germination and seedling growth in wheat and tomato. Our results suggest that inhibitor of AChE in nutgrass possibly acts as agent of plant's war against (a) herbivore animals, and (b) other plants trying to grow in the same habitat. An antiAChE from nutgrass has been purified by employing chromatography and crystallization. The structural determination of the purified inhibitor is in progress.
Sharma
Rashmi
R
Department of Botany, University of Delhi, Delhi-110007, India.
Gupta
Rajendra
R
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D029786:Cyperus / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004593:Electrophorus / Q000378:metabolism; D018525:Germination / Q000187:drug effects; D018551:Solanum lycopersicum / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D000432:Methanol; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D018520:Plant Shoots / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D036226:Seedlings / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D014908:Triticum / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development
D002800:Cholinesterase Inhibitors; D010936:Plant Extracts; D000110:Acetylcholinesterase; D000432:Methanol