Effects of the deficiency of the rhodanese-like protein RhdA in Azotobacter vinelandii.
In Azotobacter vinelandii the rhdA gene codes for a protein (RhdA) of the rhodanese-homology superfamily. By combining proteomics, enzymic profiles and ultrastructural observations, the phenotype of an A. vinelandii rhdA mutant was analyzed. We found that the A. vinelandii rhdA mutant, and not the wild-type strain, accumulated polyhydroxybutyrate. RhdA deficiency enhanced the expression of enzymes of the polyhydroxybutyrate biosynthetic operon, and affected the activity of specific tricarboxylic acid cycle enzymes. The effect was dramatic on aconitase, in spite of comparable expression of aconitase polypeptides in both strains. By using a model system, we found that RhdA triggered protection from oxidants.
Cereda
Angelo
A
Dipartimento di Scienze Molecolari Agroalimentari, Università di Milano, Milano, Italy.
Carpen
Aristodemo
A
Picariello
Gianluca
G
Iriti
Marcello
M
Faoro
Franco
F
Ferranti
Pasquale
P
Pagani
Silvia
S
D016948:Azotobacter vinelandii / Q000201:enzymology* / Q000235:genetics / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D005798:Genes, Bacterial; D008773:Methylphenazonium Methosulfate / Q000494:pharmacology; D009154:Mutation; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress / Q000235:genetics; D010641:Phenotype; D040901:Proteomics; D013884:Thiosulfate Sulfurtransferase / Q000172:deficiency / Q000235:genetics / Q000502:physiology*
D001426:Bacterial Proteins; D016877:Oxidants; D008773:Methylphenazonium Methosulfate; D013884:Thiosulfate Sulfurtransferase