Molecular serotyping of Escherichia coli O111:H8.
Accurate Escherichia coli serotyping is critical for pathogen diagnosis and surveillance of non-O157 Shiga-toxigenic strains, however few laboratories have this capacity. The molecular serotyping protocol described in this paper targets the somatic and flagellar antigens of E. coli O111:H8 used in traditional serotyping, and can be performed routinely in the laboratory.
Durso
Lisa M
LM
USDA, ARS, U.S. Meat Animal Research Center, P.O. Box 166, State Spur 18D, Clay Center, Nebraska 68933, USA. lisa.durso@ars.usda.gov
Bono
James L
JL
Keen
James E
JE
D000818:Animals; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics*; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics*; D005408:Flagellin; D006801:Humans; D019081:O Antigens / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000379:methods
D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C516095:FliC protein, E coli; D019081:O Antigens; D005408:Flagellin