Determining the repair rate for radiation-induced chromatid deletions during the first 24 hours after irradiation.
The number of chromatid plus isochromatid deletions present in the testes and bone marrow of the Chinese hamster was measured as a function of time following acute exposure to cobalt-60 gamma irradiation. The number of breaks remaining scorable at any time after irradiation exposure could be represented by a simple exponential equation (N = Noe-kt). The values of No and k, calculated after 100 roentgens of cobalt-60 gamma irradiation, were 1.15 breaks/cell and 0.16/hour, respectively, for the testes, and 0.53 break/cell and 0.14/hour, respectively, for the bone marrow. The average length of time that the breaks remain scorable, 1/k, was found to be 6.3 hours for the testes and 7.1 hours for the bone marrow. By suitable formulation, it was possible to predict the number of breaks present by using varied radiation exposure rates and times after exposure. This formulation could be used for both single and multiple radiation exposures.
Brooks
A L
AL
Department of Physical Biology, Cornell University, Ithaca, New York, USA.
Lengemann
F W
FW
D000818:Animals; D002842:Chromatids / Q000235:genetics / Q000528:radiation effects*; D002872:Chromosome Deletion; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage / Q000502:physiology*; D004260:DNA Repair / Q000502:physiology* / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays / Q000009:adverse effects*; D007700:Kinetics; D008297:Male; D008957:Models, Genetic; D011829:Radiation Dosage