Determination of sinomenine in Sinomenium acutum by capillary electrophoresis with electrochemiluminescence detection.
A Ru(bpy)(3)(2+)-based electrochemiluminescence (ECL) detection coupled with capillary electrophoresis (CE) has been established for the determination of sinomenine for the first time. Optimum separation was achieved with a fused-silica capillary column (50 cm x 25 microm i.d.) and a background electrolyte of 50 mM sodium phosphate (pH 5.0) at a separation voltage of 15 kV. The content of sinomenine was detected by ECL at the detection voltage of 1.15 V (versus Ag/AgCl) with 5 mM Ru(bpy)(3)(2+) in 75 mM phosphate solution (pH 8.0) when a chemically modified platinum electrode by europium(III)-doped prussian blue analogue (Eu-PB) was used as a working electrode. Under the optimized conditions, the ECL intensity was in proportion to sinomenine concentration in the range from 0.01 to 1.0 microg mL(-1) with a detection limit of 2.0 ng mL(-1) (3sigma). The relative standard derivations of migration time and ECL intensity were 0.93 and 1.11%, respectively. The level of sinomenine in Sinomenium acutum Rehd. et Wils was easily determined with recoveries between 98.6 and 102.7%.
Zhou
Min
M
Gansu Key Laboratory of Polymer Materials, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.
Ma
Yong-Jun
YJ
Ren
Xiao-Na
XN
Zhou
Xiu-Ying
XY
Li
Li
L
Chen
Hui
H
D002138:Calibration; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D004573:Electrolytes / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D005295:Ferrocyanides / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D049449:Luminescence; D008516:Medicine, Chinese Traditional / Q000379:methods; D009019:Morphinans / Q000032:analysis*; D010710:Phosphates / Q000494:pharmacology; D010936:Plant Extracts; D010984:Platinum / Q000737:chemistry; D012413:Rubidium / Q000737:chemistry; D012997:Solvents / Q000737:chemistry
D004573:Electrolytes; D005295:Ferrocyanides; D009019:Morphinans; D010710:Phosphates; D010936:Plant Extracts; D012997:Solvents; D010984:Platinum; C009271:sinomenine; D012413:Rubidium; C018279:sodium phosphate; C000170:ferric ferrocyanide