Characterization of a monoclonal antibody specific for human peripheral myelin protein 22 and its use in immunohistochemical studies of the fetal and adult nervous system.
We produced a mouse monoclonal antibody using cDNA and peptide immunization against the putative second extra-cellular domain of human peripheral myelin protein 22 (PMP22). It reacted specifically with human PMP22 and not with other human myelin proteins and did not react with bovine, rat, or mouse PMP22. The antibody stained the compact myelin of human peripheral nerve motor and sensory axons and did not stain central nervous system tissue. PMP22 reactivity was detected in the spinal roots of the human fetus at 19-20 weeks of gestation. The staining pattern of the PMP22 antibody resembled that of a monoclonal antibody directed against the myelin protein zero.
Gregson
Norman A
NA
Department of Clinical Neuroscience, King's College London School of Medicine, Guy's Hospital, London, UK.
Zhang
Gaiping
G
Pritchard
Jane
J
Wang
Aiping
A
Sanvito
Lara
L
Hayday
Adrian C
AC
Hughes
Richard A C
RA
D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D000918:Antibody Specificity; D002461:Cell Line, Transformed; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005333:Fetus; D006801:Humans; D006825:Hybridomas / Q000502:physiology; D007074:Immunoglobulin G / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods*; D051379:Mice; D008822:Mice, Transgenic; D018993:Myelin P0 Protein / Q000378:metabolism; D009185:Myelin Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D009420:Nervous System / Q000378:metabolism*; D014162:Transfection / Q000379:methods
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D018993:Myelin P0 Protein; D009185:Myelin Proteins; C073687:PMP22 protein, human