title: The influence of the immunostimulation by bacterial cell components derived from altered large intestinal microbiota on probiotic anti-inflammatory benefits.
abstract: Using murine macrophage-like J774.1 cells and fecal precipitates prepared from the feces of elderly volunteers whose acute inflammation had been inhibited by LKM512 yogurt consumption, we investigated the likelihood that immunostimulation by altered intestinal bacterial cell components contribute to the anti-inflammatory benefits of this yogurt. Tumor necrosis factor-alpha production due to stimulation by fecal precipitates obtained during LKM512 yogurt consumption tended to be higher than due to stimulation by precipitates obtained from preconsumption (P=0.0827), although acute phase response was suppressed by LKM512 yogurt consumption. We suggest that the anti-inflammatory benefits of LKM512 yogurt on elderly volunteers are independent of direct immunostimulation by the bacterial cell components derived from altered intestinal microbiota.
authors: Dairy Science and Technology Institute, Kyodo Milk Industry Co. Ltd, Hirai, Hinode, Tokyo, Japan. m-matumoto@meito.co.jp
authors: Mitsuharu
authors: M
authors: Matsumoto
authors: Kurt
authors: K
authors: Hara
authors: Yoshimi
authors: Y
authors: Benno
mesh_terms: D000368:Aged; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001419:Bacteria / Q000276:immunology*; D002460:Cell Line; D005243:Feces / Q000382:microbiology; D005260:Female; D041981:Gastrointestinal Tract / Q000382:microbiology*; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis; D053582:Interleukin-1alpha / Q000096:biosynthesis; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D019936:Probiotics / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D015014:Yogurt
chemical_list: D000893:Anti-Inflammatory Agents; D053582:Interleukin-1alpha; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10