An electrochemical on-field sensor system for the detection of compost maturity.
A maturity sensor system was developed, based on the combination of three electrically measured parameters, pH, NH(4)(+) concentration, and phosphatase activity in the water extracts of compost samples. One of these parameters, the apparent phosphatase activity in crude test solutions was determined using screen-printed carbon strips (SPCSs) coated with alpha-naphthyl phosphate (alpha-NP) in Nafion film. The phosphatase activity was monitored in connection with differential pulse voltammetry (DPV) with an aliquot (30 microL) of the test solution on SPCS. The phosphatase activity sensor was validated using alkaline phosphatase (ALP) in Tris-HCl buffer (pH 8.0) and acid phosphatase (ACP) in citric acid buffer (pH 5.0). The activity of the spiked enzymes in the water extract of the compost sample could be confirmed with the change of corresponding oxidation peak current signal of the product, alpha-naphthol. The water extracts of compost samples (n=24) collected in various composting days were applied to our compost maturity sensor system, and the conventional germination tests. Using multiple regression analysis, the germination index (GI) was expressed by the multi-linear regression equation consisting of pH, NH(4)(+) concentration, and the phosphatase activity. The calculated GI from the regression equation had a good correlation with the measured GI of the corresponding composts (r=0.873). As a result, we have determined an equation for the determination of the compost stability using our portable sensor system rapidly at the composting site.
Chikae
Miyuki
M
School of Materials Science, Japan Advanced Institute of Science and Technology (JAIST), 1-1 Asahidai, Nomi City, Ishikawa 923-1292, Japan.
Kerman
Kagan
K
Nagatani
Naoki
N
Takamura
Yuzuru
Y
Tamiya
Eiichi
E
D004563:Electrochemistry / Q000295:instrumentation*; D018525:Germination; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D012639:Seeds; D012987:Soil
D012987:Soil; D010744:Phosphoric Monoester Hydrolases