Suppression subtractive hybridization identifies novel transcripts in regenerating Hydra littoralis.
Despite considerable interest in the biologic processes of regeneration and stem cell activation, little is known about the genes involved in these transformative events. In a Hydra littoralis model of regeneration, we employed a rapid shotgun suppression subtractive hybridization strategy to identify genes that are uniquely expressed in regenerating tissue. With an adaptor-PCR based technique, 16 candidate transcripts were identified, 15 were confirmed unique to mRNA isolated from hydra undergoing regeneration. Of these, 6 were undescribed in GenBank and allied expressed sequence tag (EST) databases (GenBank + EMBL + DDBJ + PDB and the Hydra EST database). BLAST analysis of these sequences identified remarkably similar sequences in anonymous ESTs found in a wide variety of animal species.
Stout
Thomas
T
Casey Eye Institute, Oregon Health and Science University, 3375 SW Terwilliger Blvd, Portland, OR 97239, USA.
McFarland
Trevor
T
Appukuttan
Binoy
B
D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D020224:Expressed Sequence Tags; D006829:Hydra / Q000235:genetics* / Q000502:physiology*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012038:Regeneration
D018076:DNA, Complementary; D012333:RNA, Messenger