title: OxLDL-induced gene expression patterns in CASMC are mimicked in apoE-/- mice aortas.
abstract: Oxidized low density lipoprotein (oxLDL) contributes to the pathophysiology of atherosclerosis, partly by altering gene expression in vascular cells. Here, we show 221 genes differentially regulated by oxLDL in coronary artery smooth muscle cells (CASMC), using oligonucleotide microarrays. These genes were classified into 14 functional groups. A comparable gene expression pattern was detected in apoE(-/-) mice. OxLDL induced an oxidative stress response in CASMC, but not the unfolded protein response. OxLDL also caused CASMC death which was accompanied by increased expression of FasL, Bax, and p53 but was caspase-independent. This approach provides further insight into disease pathology and prognosis.
authors: Department of Biochemistry, National University of Ireland, Galway, Ireland.
authors: Janice L V
authors: JL
authors: Reeve
authors: Catherine
authors: C
authors: Stenson-Cox
authors: Aideen
authors: A
authors: O'Doherty
authors: Isabella
authors: I
authors: Pörn-Ares
authors: Mikko
authors: M
authors: Ares
authors: Timothy
authors: T
authors: O'Brien
authors: Afshin
authors: A
authors: Samali
mesh_terms: D000818:Animals; D001011:Aorta / Q000502:physiology; D001057:Apolipoproteins E / Q000172:deficiency*; D020169:Caspases / Q000502:physiology; D016923:Cell Death / Q000502:physiology; D003331:Coronary Vessels / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D020869:Gene Expression Profiling; D006801:Humans; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D051379:Mice; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D018384:Oxidative Stress / Q000502:physiology
chemical_list: D001057:Apolipoproteins E; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D020169:Caspases