title: Haloferax larsenii sp. nov., an extremely halophilic archaeon from a solar saltern.
abstract: Three strains of Gram-negative, aerobic, neutrophilic, extremely halophilic archaea, designated ZJ206(T), ZJ203 and ZJ204, were isolated from a solar saltern in Zhe-Jiang Province, China. Phenotypically and on the basis of 16S rRNA gene sequences, the strains were very similar. Comparative 16S rRNA gene analysis revealed 96.4-97.4 % sequence similarity to members of the genus Haloferax. The major polar lipids were C(20)C(20) derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, diglycosyl glycerol diether and sulfated diglycosyl diether. The DNA G+C content of strain ZJ206(T) was 62.2 mol%. The results of DNA-DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of the isolates from closely related species. Therefore the isolates should be classified as members of a novel species, for which the name Haloferax larsenii sp. nov. is proposed. The type strain is ZJ206(T) (=CGMCC 1.5347(T)=JCM 13917(T)).
authors: Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.|College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors: Xue-Wei
authors: XW
authors: Xu
authors: College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors: Yue-Hong
authors: YH
authors: Wu
authors: Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, People's Republic of China.
authors: Chun-Sheng
authors: CS
authors: Wang
authors: Institute of Life Sciences and the Moshe Shilo Minerva Centre for Marine Biogeochemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
authors: Aharon
authors: A
authors: Oren
authors: Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.
authors: Pei-Jin
authors: PJ
authors: Zhou
authors: College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors: Min
authors: M
authors: Wu
mesh_terms: D002681:China; D019641:DNA, Archaeal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D019621:Haloferax / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012965:Sodium Chloride; D012988:Soil Microbiology; D014871:Water Microbiology
chemical_list: D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride