Nocardioides marinus sp. nov.
A Gram-positive, slightly halophilic, rod-shaped bacterium, designated CL-DD14(T), was isolated from seawater of the East Sea, Korea. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain CL-DD14(T) belongs to the genus Nocardioides. Levels of 16S rRNA gene sequence similarity between strain CL-DD14(T) and the type strains of recognized species of the genus Nocardioides were low (94.1-97.1 %). Strain CL-DD14 (T) grew over the pH range 6-9 and temperature range 10-40 degrees C. The strain grew at NaCl concentrations of 0.5-8 % (w/v) with optimum growth at 1-3 % and no growth was observed after 3 weeks on nutrient agar without any salts. It contained ll-diaminopimelic acid as the diamino acid in the cell wall. The major isoprenoid quinone was MK-8(H(4)) and the major cellular fatty acids were iso-C(16 : 0) and 10-methyl C(17 : 0). The DNA G+C content was 72.9 mol%. On the basis of phenotypic and phylogenetic data, strain CL-DD14(T) is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioides marinus sp. nov. is proposed. The type strain is CL-DD14(T) (=KCCM 42321(T)=DSM 18248(T)).
Choi
Dong Han
DH
Marine Environmental Research Department, Korea Ocean Research and Development Institute, Ansan 426-744, Republic of Korea.
Kim
Hye Min
HM
School of Earth and Environmental Sciences, Seoul National University, Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
Noh
Jae-Hoon
JH
Marine Environmental Research Department, Korea Ocean Research and Development Institute, Ansan 426-744, Republic of Korea.
Cho
Byung Cheol
BC
School of Earth and Environmental Sciences, Seoul National University, Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D007723:Korea; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S