In vitro antimicrobial activity of different gutta-percha points and calcium hydroxide pastes.
The aim of this study was to evaluate the antimicrobial activity of different trademarks and compositions of gutta-percha points and calcium hydroxide pastes used in endodontic therapy. The evaluated material consisted of gutta-percha points containing calcium hydroxide (Roeko), gutta-percha points containing chlorhexidine (Roeko), two conventional gutta-percha points (Endo Points and Roeko) and two calcium hydroxide pastes (Calen and Calen/PMCC). Antimicrobial tests included five species of microorganisms: Escherichia coli (ATCC10538), Staphylococcus epidermidis (ATCC12228), Staphylococcus aureus (ATCC6538), Pseudomonas aeruginosa (ATCC27853), and Micrococcus luteus (ATCC9341). The Agar diffusion method was employed. The plates were kept at room temperature for 2 h for prediffusion and then incubated at 37 degrees C for 24 h. The triphenyltetrazolium chloride gel was added for optimization and the zones of inhibition were measured. Statistical evaluation was carried out using analysis of variance and Tukey Test. The obtained results showed that all microbial species used in the study were inhibited by the gutta-percha points containing chlorhexidine and by the calcium hydroxide pastes (Calen and Calen/PMCC), with similar results (p > 0.05). No antimicrobial activity was observed for the other groups. It was concluded that the gutta-percha points containing chlorhexidine presented antimicrobial activity, whereas the gutta-percha points containing calcium hydroxide did not.
Tanomaru
Juliane Maria Guerreiro
JM
School of Dentistry of Araraquara, State University of São Paulo, Rua Expedicionários do Brazil 2030, Araraquara, SP, Brazil, CEP 14801-360. jutanomaru@uol.com.br
Pappen
Fernanda Geraldes
FG
Tanomaru Filho
Mário
M
Spolidorio
Denise Madalena Palomari
DM
Ito
Izabel Yoko
IY
D000704:Analysis of Variance; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D002126:Calcium Hydroxide / Q000494:pharmacology*; D002710:Chlorhexidine / Q000494:pharmacology*; D004708:Endodontics; D004926:Escherichia coli / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D006171:Gutta-Percha / Q000592:standards; D016982:Micrococcus luteus / Q000187:drug effects; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D013212:Staphylococcus epidermidis / Q000187:drug effects; D014092:Tooth Root / Q000187:drug effects / Q000382:microbiology
D000891:Anti-Infective Agents, Local; D006171:Gutta-Percha; D002126:Calcium Hydroxide; D002710:Chlorhexidine