Effects of phthalate esters on dendritic cell subsets and interleukin-4 production in fluorescein isothiocyanate-induced contact hypersensitivity.
Phthalate esters with short alkyl chains, such as di-ethyl (DEP), di-n-propyl (DPP), and di-butyl phthalate (DBP), have adjuvant effects on an FITC-induced contact hypersensitivity mouse model. The adjuvant effects of DPP and DBP are associated with enhanced trafficking of FITC-presenting CD11b(+) dendritic cells (DC). DEP has relatively weak activity as to FITC-positive cell migration. Here we demonstrated that DBP and DPP also increased the number of FITC-positive CD8alpha(+) DC in draining lymph nodes. We also found enhanced production of interleukin-4 in draining lymph nodes after FITC sensitization with DEP, DPP, or DBP, suggesting an additional adjuvant mechanism of phthalate esters.
Maruyama
Takashi
T
Laboratory of Microbiology and Immunology and COE Program in the 21st Century, University of Shizuoka School of Pharmaceutical Sciences, Shizuoka, Japan.
Shiba
Takahiro
T
Iizuka
Hiromi
H
Matsuda
Tomoko
T
Kurohane
Kohta
K
Imai
Yasuyuki
Y
D000818:Animals; D016827:CD8 Antigens / Q000276:immunology; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D003877:Dermatitis, Contact / Q000209:etiology / Q000276:immunology* / Q000378:metabolism; D003993:Dibutyl Phthalate / Q000494:pharmacology*; D005260:Female; D016650:Fluorescein-5-isothiocyanate / Q000008:administration & dosage / Q000378:metabolism; D015847:Interleukin-4 / Q000096:biosynthesis* / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000276:immunology; D008198:Lymph Nodes / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008813:Mice, Inbred ICR; D010795:Phthalic Acids / Q000494:pharmacology*; D010968:Plasticizers / Q000494:pharmacology
D016827:CD8 Antigens; C431698:CD8alpha antigen; D010795:Phthalic Acids; D010968:Plasticizers; D015847:Interleukin-4; D003993:Dibutyl Phthalate; C106479:di-n-propylphthalate; D017493:Leukocyte Common Antigens; D016650:Fluorescein-5-isothiocyanate