title: N,N'-Bis-(2-(cyano)ethoxycarbonyl)-2-methyl-2-thiopseudourea: a guanylating reagent for synthesis of 2'-O-[2-(Guanidinium)ethyl]-modified oligonucleotides.
abstract: A guanylating reagent, N,N'-bis-(2-(cyano)ethoxycarbonyl)-2-thiopseudourea, was synthesized and used for synthesis of 2'-O-[2-(guanidinium)ethyl] (2'-O-GE) modified oligonucleotides. A convenient deprotection method for the 2'-O-GE oligonucleotides was developed.
authors: Department of Medicinal Chemistry, Isis Pharmaceuticals Inc., Carlsbad, California 92008. USA.
authors: Thazha P
authors: TP
authors: Prakash
authors: Ask
authors: A
authors: Püschl
authors: Muthiah
authors: M
authors: Manoharan
mesh_terms: D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D009841:Oligonucleotides / Q000138:chemical synthesis*; D013890:Thiourea / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: C519049:N,N'-bis-(2-(cyano)ethoxycarbonyl)-2-methyl-2-thiopseudourea; D009841:Oligonucleotides; D013890:Thiourea