Adhesin-dependent binding and uptake of Salmonella enterica serovar Typhimurium by dendritic cells.
Salmonella enterica serovar Typhimurium can be internalized by immature dendritic cells (DCs). The interacting host and bacterial molecules initiating this process remain uncharacterized. The objective of this study was to investigate whether specific fimbriae are involved in the early step of binding and uptake of Salmonella by DCs. Type 1 fimbriated S. enterica serovar Typhimurium or recombinant Escherichia coli expressing the type 1 fimbriae showed a significantly greater ability to attach to murine bone-marrow-derived DCs than non-fimbriated bacteria. The FimH adhesin was required for efficient interactions with DCs, since fimbriated fimH mutants were impaired in both binding and internalization. Finally, the internalization involved a FimH-dependent process but did not require sipB, a gene essential for Salmonella-mediated invasion of mammalian epithelial cells. Collectively, these data suggest that the bacterial interaction of DCs through the type 1 fimbrial adhesin FimH is sufficient to target S. enterica serovar Typhimurium for cellular uptake.
Guo
Aizhen
A
University of Pennsylvania School of Veterinary Medicine, 3800 Spruce Street, Philadelphia, PA 19104, USA.
Lasaro
Melissa A
MA
University of Pennsylvania School of Veterinary Medicine, 3800 Spruce Street, Philadelphia, PA 19104, USA.
Sirard
Jean-Claude
JC
Swiss Institute for Experimental Cancer Research (ISREC), 1066 Epalinges, Switzerland.
Kraehenbühl
Jean-Pierre
JP
Swiss Institute for Experimental Cancer Research (ISREC), 1066 Epalinges, Switzerland.
Schifferli
Dieter M
DM
University of Pennsylvania School of Veterinary Medicine, 3800 Spruce Street, Philadelphia, PA 19104, USA.
D018829:Adhesins, Bacterial / Q000378:metabolism*; D018830:Adhesins, Escherichia coli; D000818:Animals; D001422:Bacterial Adhesion; D001854:Bone Marrow Cells / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D036781:Fimbriae Proteins / Q000378:metabolism; D010861:Fimbriae, Bacterial / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D012486:Salmonella typhimurium / Q000276:immunology / Q000378:metabolism*
D018829:Adhesins, Bacterial; D018830:Adhesins, Escherichia coli; D036781:Fimbriae Proteins