title: Multivariate segregation analysis for quantitative traits in line crosses.
abstract: Segregation analysis is a method of detecting major genes for quantitative traits without using marker information. It serves as an important tool in helping investigators to plan further studies such as quantitative trait loci mapping or more sophisticated genomic analyses. However, current methods of segregation analysis for a single trait typically have low statistical power. We propose a multivariate segregation analysis (MSA) that takes advantage of the correlation structure of multiple quantitative traits to detect major genes. This method not only increases the statistical power, but allows dissection of the genetic architecture underlying the trait complex. In MSA the observed phenotypes of multiple correlated traits are fitted to a multivariate Gaussian mixture model. Model parameters are estimated under the maximum likelihood framework via the expectation-maximization algorithm. The presence of major genes is tested using likelihood ratio test statistics. Pleiotropy is distinguished from close linkage by comparing three possible models using the Bayesian information criterion. Two simulation experiments were performed based on the F(2) mating design. In the first, the statistical properties of MSA under varying heritabilities and sample sizes were investigated and the results compared with those obtained from single-trait analysis. In the second simulation the efficacy of MSA in separating pleiotropy from close linkage was demonstrated. Finally, the new method was applied to real data and detected a major gene responsible for both plant height and tiller number in rice.
authors: Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Key Laboratory of Plant Functional Genomics of Ministry of Education, Yangzhou University, Yangzhou, China.
authors: J
authors: J
authors: Xiao
authors: X
authors: X
authors: Wang
authors: Z
authors: Z
authors: Hu
authors: Z
authors: Z
authors: Tang
authors: C
authors: C
authors: Xu
mesh_terms: D020090:Chromosome Segregation / Q000235:genetics*; D003198:Computer Simulation; D003433:Crosses, Genetic; D005787:Gene Frequency; D017343:Genes, Plant; D008040:Genetic Linkage; D008957:Models, Genetic; D015999:Multivariate Analysis; D016011:Normal Distribution; D012275:Oryza / Q000235:genetics; D040641:Quantitative Trait Loci