title: Rapid hyperspectral fluorescence lifetime imaging.
abstract: We report a rapid hyperspectral fluorescence lifetime imaging (FLIM) instrument that exploits high-speed FLIM technology in a line-scanning microscope. We demonstrate the acquisition of whole-field optically sectioned hyperspectral fluorescence lifetime image stacks (with 32 spectral bins) in less than 40 s and illustrate its application to unstained biological tissue.
authors: Department of Physics, Imperial College London, South Kensington Campus, London, SW7 2AZ, United Kingdom.
authors: Pieter
authors: P
authors: De Beule
authors: Dylan M
authors: DM
authors: Owen
authors: Hugh B
authors: HB
authors: Manning
authors: Clifford B
authors: CB
authors: Talbot
authors: Jose
authors: J
authors: Requejo-Isidro
authors: Christopher
authors: C
authors: Dunsby
authors: James
authors: J
authors: McGinty
authors: Richard K P
authors: RK
authors: Benninger
authors: Daniel S
authors: DS
authors: Elson
authors: Ian
authors: I
authors: Munro
authors: M
authors: M
authors: John Lever
authors: Praveen
authors: P
authors: Anand
authors: Mark A A
authors: MA
authors: Neil
authors: Paul M W
authors: PM
authors: French
mesh_terms: D001158:Arteries / Q000737:chemistry / Q000648:ultrastructure; D031405:Convallaria / Q000737:chemistry / Q000648:ultrastructure; D003952:Diagnostic Imaging / Q000379:methods*; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D013997:Time Factors