Expression of the cytoskeleton linker protein ezrin in human cancers.
Expression of the metastasis-associated protein, ezrin, in over 5,000 human cancers and normal tissues was analyzed using tissue microarray immunohistochemistry. Ezrin staining was compared between cancers and their corresponding normal tissues, between cancers of epithelial and mesenchymal origin, in the context of the putative inhibitor protein, merlin, and against clinicopathological data available for breast, lung, prostate cancers and sarcomas. Ezrin was found in most cancers and normal tissues at varying levels of intensity. In general ezrin was expressed at higher levels in sarcomas than in carcinomas. By normalizing the expression of ezrin in each cancer using ezrin expression found in the corresponding normal tissue, significant associations between ezrin were found in advancing histological grade in sarcomas (P = 0.02) and poor outcome in breast cancer (P = 0.025). Clinicopathologic associations were not changed by simultaneous assessment of ezrin and merlin in each patient sample for the cancer types examined. These data support a role for ezrin in the biology of human cancers and the need for additional studies in breast cancer and sarcoma patients that may validate ezrin as a marker of cancer progression and as a potential target for cancer therapy.
Bruce
Benjamin
B
Tumor and Metastasis Biology Section, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA.
Khanna
Gaurav
G
Ren
Ling
L
Landberg
Goran
G
Jirström
Karin
K
Powell
Charles
C
Borczuk
Alain
A
Keller
Evan T
ET
Wojno
Kirk J
KJ
Meltzer
Paul
P
Baird
Kristin
K
McClatchey
Andrea
A
Bretscher
Anthony
A
Hewitt
Stephen M
SM
Khanna
Chand
C
D045744:Cell Line, Tumor; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D009369:Neoplasms / Q000378:metabolism*; D046888:Tissue Array Analysis; D000099149:Ezrin
D003598:Cytoskeletal Proteins; D000099149:Ezrin