title: The organic cation transporters (OCT1, OCT2, EMT) and the plasma membrane monoamine transporter (PMAT) show differential distribution and cyclic expression pattern in human endometrium and early pregnancy decidua.
abstract: The non-neuronal monoamine transporters (OCT1, OCT2, EMT, and PMAT) play a key role in the clearance of monoamines from extracellular compartments. In a previous report we described endometrial distribution and cyclic variation of the vesicular monoamine transporter (VMAT2) mRNA and the neuronal norepinephrine transporter (NET) mRNA. In the present study we used in situ hybridization, real-time PCR and immunohistochemistry to reveal tissue distribution and cyclic variation of mRNA for the non-neuronal monoamine transporters in the human endometrium and early pregnancy decidua. We found that non-neuronal monoamine transporters are predominantly expressed in the stroma. The plasma membrane monoamine transporter (PMAT) mRNA expression peaked in the proliferative phase, whereas the extra-neuronal monoamine transporter (EMT) mRNA expression peaked in the secretory phase. The organic cation transporter 2 (OCT2) mRNA expression was exclusively detected in few scattered stromal cells and OCT1 mRNA was not detected at all. Our present results demonstrate that PMAT, EMT, and OCT2 transporters are expressed in the endometrial stroma and can potentially regulate reuptake of monoamines in general and histamine in particular. Taken together with our previous finding of VMAT2 mRNA in epithelial cells, we suggest a paracrine interaction between stromal and epithelial cells, which may modulate certain steps of the reproductive process.
authors: Department of Obstetrics and Gynecology, Lund University Hospital, Lund, Sweden.
authors: Barbara
authors: B
authors: Bottalico
authors: Vera
authors: V
authors: Noskova
authors: Radovan
authors: R
authors: Pilka
authors: Irene
authors: I
authors: Larsson
authors: Henryk
authors: H
authors: Domanski
authors: Bertil
authors: B
authors: Casslén
authors: Stefan R
authors: SR
authors: Hansson
mesh_terms: D000328:Adult; D003656:Decidua / Q000378:metabolism*; D004717:Endometrium / Q000378:metabolism*; D033705:Equilibrative Nucleoside Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D050811:Octamer Transcription Factor-1 / Q000235:genetics / Q000378:metabolism; D027701:Organic Cation Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D000075102:Organic Cation Transporter 2; D010507:Periodicity; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D014018:Tissue Distribution; D000099254:Organic Cation Transporter 3
chemical_list: D033705:Equilibrative Nucleoside Transport Proteins; D050811:Octamer Transcription Factor-1; D027701:Organic Cation Transport Proteins; D000075102:Organic Cation Transporter 2; D000099254:Organic Cation Transporter 3; C494165:POU2F1 protein, human; C491013:SLC22A2 protein, human; C495988:SLC29A4 protein, human