Multiplex SNP discrimination.
Multiplex hybridization reactions of perfectly matched duplexes and duplexes containing a single basepair mismatch (SNPs) were investigated on DNA microarrays. Effects of duplex length, G-C percentage, and relative position of the SNP on duplex hybridization and SNP resolution were determined. Our theoretical model of multiplex hybridization accurately predicts observed results and implicates target concentration as a critical variable in multiplex SNP detection.
Fish
Daniel J
DJ
Horne
M Todd
MT
Searles
Robert P
RP
Brewood
Greg P
GP
Benight
Albert S
AS
D001483:Base Sequence; D004252:DNA Mutational Analysis / Q000379:methods*; D016002:Discriminant Analysis; D017403:In Situ Hybridization / Q000379:methods*; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods*