title: Oxidative DNA damage and antioxidant activity in patients with inflammatory bowel disease.
abstract: Chronic inflammation may contribute to cancer risk through the accumulation of specific products as a result of DNA damage. Endogenous antioxidant enzymes prevent the formation of these harmful products. Oxidative DNA damage and endogenous antioxidant defense were determined in patients with inflammatory bowel disease (IBD). Plasma levels of 8-hydroxydeoxyguanosine (8-OHdG) and nitric oxide (NO) and plasma activities of glutathione peroxidase (G-Px) and superoxide dismutase (SOD) were determined in patients with IBD by ELISA and spectrophotometric assay, respectively. Plasma levels of 8-OHdG, SOD, and G-Px activity were found to be increased in the patient group compared to the control group (P < 0.02, P < 0.001, and P < 0.001, respectively), whereas NO was unchanged. 8-OHdG level was found to be weakly correlated with age, NO, and SOD. The results show increased DNA damage in patients with IBD. This may explain the increased risk of developing colon cancer in these patients.
authors: Department of Biochemistry, Istanbul University, Dere Sok., Umut Ap No 21/44, Sahrayi Cedid, Erenkoy, Istanbul, Turkey. stare63@yahoo.com
authors: Yildiz
authors: Y
authors: Dincer
authors: Yusuf
authors: Y
authors: Erzin
authors: Solen
authors: S
authors: Himmetoglu
authors: Kezban Nur
authors: KN
authors: Gunes
authors: Kadir
authors: K
authors: Bal
authors: Tülay
authors: T
authors: Akcay
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D004249:DNA Damage / Q000502:physiology*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000097:blood; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000097:blood* / Q000201:enzymology / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000097:blood; D018384:Oxidative Stress / Q000502:physiology*; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D000975:Antioxidants; D009569:Nitric Oxide; D000080242:8-Hydroxy-2'-Deoxyguanosine; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D003849:Deoxyguanosine