Expression of a liver-specific function by a hepatoblastoma cell line cocultured with three-dimensional endothelialized tubes in collagen gels.
A human hepatoblastoma cell line, Hep G2, showed albumin production activity, a hepatic function, when cocultured with three-dimensional endothelialized tubes in collagen gels. The albumin production rate of the collagen-based liver-like constructs increased with increasing length of the endothelialized tube in the construct.
Takei
Takayuki
T
Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0385, Japan. ttakei@chem-eng.kyushu-u.ac.jp
Sakai
Shinji
S
Ijima
Hiroyuki
H
Kawakami
Koei
K
D000418:Albumins / Q000032:analysis / Q000096:biosynthesis*; D045744:Cell Line, Tumor; D018920:Coculture Techniques; D003094:Collagen / Q000737:chemistry / Q000378:metabolism; D004727:Endothelium / Q000254:growth & development* / Q000648:ultrastructure; D005782:Gels / Q000737:chemistry; D018197:Hepatoblastoma; D006801:Humans; D008099:Liver / Q000166:cytology / Q000502:physiology*; D008855:Microscopy, Electron, Scanning; D009924:Organ Culture Techniques; D023822:Tissue Engineering / Q000379:methods*
D000418:Albumins; D005782:Gels; D003094:Collagen