title: Biosensor immunoassay for flumequine in broiler serum and muscle.
abstract: Flumequine (Flu) is one of the fluoroquinolones most frequently applied for the treatment of broilers in The Netherlands. For the detection of residues of Flu in blood serum of broilers, a biosensor immunoassay (BIA) was developed which was fast (7.5 min per sample) and specific (no cross-reactivity with other (fluoro)quinolones). This inhibition assay was based on a rabbit polyclonal anti-Flu serum and a CM5 biosensor chip coated with Flu which could be detected in the range of 15-800 ng mL(-1). For the detection of Flu in muscle, an easy extraction procedure in buffer was selected and the measuring range was from 24 to 4000 ng g(-1). Average recoveries of 66 till 75% were found with muscle samples spiked at 0.5, 1 and 2 times the maximum residue limit (MRL in muscle = 400 ng g(-1)) and the decision limit (CCalpha) and the detection capability (CCbeta) were determined as 500 and 600 ng g(-1), respectively. Incurred muscle samples were analysed by the BIA and by LC-MS/MS and a good correlation was found (R2 = 0.998). Serum and muscle samples from with Flu treated broilers were analysed and the concentrations found in serum were always higher than those found in muscle (average serum/muscle ratio was 3.5) and this proved the applicability of the BIA in serum as predictor of the Flu concentration in muscle.
authors: RIKILT-Institute of Food Safety, P.O. Box 230, 6700 AE Wageningen, The Netherlands. willem.haasnoot@wur.nl
authors: Willem
authors: W
authors: Haasnoot
authors: Hüsniye
authors: H
authors: Gerçek
authors: Geert
authors: G
authors: Cazemier
authors: Michel W F
authors: MW
authors: Nielen
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis; D015374:Biosensing Techniques; D002645:Chickens; D002853:Chromatography, Liquid; D004350:Drug Residues / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D024841:Fluoroquinolones / Q000032:analysis*; D005504:Food Analysis / Q000379:methods; D007118:Immunoassay / Q000379:methods*; D013058:Mass Spectrometry; D008956:Models, Chemical; D009132:Muscles / Q000378:metabolism*; D011817:Rabbits; D020349:Surface Plasmon Resonance
chemical_list: D000890:Anti-Infective Agents; D024841:Fluoroquinolones; C012976:flumequine