title: Conditioned genome reconstruction: how to avoid choosing the conditioning genome.
abstract: Genome phylogenies can be inferred from data on the presence and absence of genes across taxa. Logdet distances may be a good method, because they allow expected genome size to vary across the tree. Recently, Lake and Rivera proposed conditioned genome reconstruction (calculation of logdet distances using only those genes present in a conditioning genome) to deal with unobservable genes that are absent from every taxon of interest. We prove that their method can consistently estimate the topology for almost any choice of conditioning genome. Nevertheless, the choice of conditioning genome is important for small samples. For real bacterial genome data, different choices of conditioning genome can result in strong bootstrap support for different tree topologies. To overcome this problem, we developed supertree methods that combine information from all choices of conditioning genome. One of these methods, based on the BIONJ algorithm, performs well on simulated data and may have applications to other supertree problems. However, an analysis of 40 bacterial genomes using this method supports an incorrect clade of parasites. This is a common feature of model-based gene content methods and is due to parallel gene loss.
authors: Department of Mathematics and Statistics, Dalhousie University, Hali, Nova Scotia, B3H 3J5, Canada. m.spenser@liverpool.ac.uk
authors: Matthew
authors: M
authors: Spencer
authors: David
authors: D
authors: Bryant
authors: Edward
authors: E
authors: Susko
mesh_terms: D000465:Algorithms; D001419:Bacteria / Q000235:genetics; D002965:Classification / Q000379:methods*; D003198:Computer Simulation; D005796:Genes / Q000235:genetics*; D016678:Genome / Q000235:genetics*; D008957:Models, Genetic; D010802:Phylogeny