Chip-based CE coupled to a quadrupole TOF mass spectrometer for the analysis of a glycopeptide.
A novel approach using sheath flow chip-based CE coupled to a quadrupole TOF mass spectrometer was developed and applied to the analysis of an O-glycopeptide. The method provided good separation and sensitive analysis of the glycopeptide and its beta-elimination product. The beta-elimination reaction of the glycopeptide with ammonia or dimethylamine was adapted for changing the glycopeptide into deglycosylated form and for specifying the site of glycosylation. MS/MS analysis of the native peptide, glycopeptide and beta-elimination product enabled the identification of the glycosylation site through the comparison of the fragmentations and peptide sequence analysis.
Zheng
Yufang
Y
Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong SAR, P. R. China.
Li
Haifang
H
Guo
Zhihong
Z
Lin
Jin-Ming
JM
Cai
Zongwei
Z
D019342:Acetic Acid / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D046209:Electrophoresis, Microchip / Q000295:instrumentation* / Q000379:methods; D006020:Glycopeptides / Q000032:analysis* / Q000737:chemistry; D006031:Glycosylation; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D000432:Methanol / Q000737:chemistry; D044085:Microfluidics / Q000145:classification / Q000379:methods*; D009844:Oligosaccharides / Q000737:chemistry; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D006020:Glycopeptides; D009844:Oligosaccharides; D019342:Acetic Acid; D000432:Methanol