[Genetic polymorphism of ten X-STR loci in naxi population].
We studied the genetic polymorphism and haplotypes of ten short tandem repeats (STR) loci on the X chromosome in Yunnan Naxi population. Genotyping and detection of PCR products from the ten X-STRs were carried out on denaturing polyacrylamide gel electrophoresis followed by silver staining. The results revealed that among 98 males from Yunnan Naxi population, the number of alleles in the 10 loci (DXS7423, DXS7424, DXS6799, DXS7133, DXS6804, DXS8378, HPRTB, DXS7130, DXS7132 and DXS6789) were 4, 7, 6, 3, 6, 5, 5, 7, 6 and 8, respectively. Gene frequencies ranged from 0.0102 (DXS7132 and DXS6789) to 0.7347 (DXS7133). A total of 20 haplotypes were detected in DXS8378 and DXS7130 loci, and 56 haplotypes were detected in DXS6789, DXS6799 and DXS7424 loci. The haplotype diversity reached 0.8553 and 0.9649, respectively. In conclusion, the 10 X-STR loci are relatively abundant in polymorphic information for forensic identification, paternity testing and for genetic population studies.
Chen
Teng
T
Department of Forensic Medicine, Xi'an Jiaotong University School of Medicine, Xi'an, China. chenteng@mail.xjtu.edu.cn
Xin
Na
N
Zhu
Jun-Yan
JY
Yu
Bing
B
Jin
Tan-Bo
TB
Li
Sheng-Bin
SB
D002681:China; D041321:Chromosomes, Human, X / Q000235:genetics; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*