Suppression of immune-mediated liver injury after vaccination with attenuated pathogenic cells.
Cell vaccination via immunization with attenuated pathogenic cells is an effective preventive method that has been successfully applied in several animal models of inflammatory or autoimmune diseases. Concanavalin A (Con A)-induced hepatitis (CIH) is a commonly used experimental model to study immune-mediated liver injury. Multiple cell types including T lymphocytes, macrophages and neutrophils have been found to be involved in the pathogenesis of CIH. In this study, we used attenuated spleen lymphocytes or peripheral blood lymphocytes as vaccines to investigate whether they could induce protective immune responses to prevent mice from developing CIH. We found that mice receiving such vaccination before CIH induction developed much milder diseases, exhibited a lower level of alanine aminotransferase (ALT) released into their plasma and had less inflammatory lesions in their livers. Such CIH-suppression is dose- and frequency-dependent. The suppressive effect was associated with inhibition of several major inflammatory mediators, pro-inflammatory cytokines and chemokines.
Mei
Yunhua
Y
Shanghai Institute of Immunology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China.
Wang
Ying
Y
Xu
Lingyun
L
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D003208:Concanavalin A / Q000009:adverse effects; D016207:Cytokines / Q000276:immunology; D004195:Disease Models, Animal; D004306:Dose-Response Relationship, Immunologic; D006520:Hepatitis, Animal / Q000139:chemically induced / Q000276:immunology* / Q000628:therapy*; D008099:Liver / Q000473:pathology; D017710:Lymphocyte Transfusion; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000276:immunology*; D014611:Vaccination / Q000379:methods; D014612:Vaccines / Q000627:therapeutic use*
D016207:Cytokines; D014612:Vaccines; D003208:Concanavalin A; D000410:Alanine Transaminase