Media optimization for the production of beta-carotene by Blakeslea trispora: a statistical approach.
Blakeslea trispora (+) MTCC, Blakeslea trispora NRRL 2895 (+), Blakeslea trispora NRRL 2896 (-) as well as intraspecific mating of both the strain types have been studied for optimum production of beta-carotene. Intraspecific mating of both the strain types increased the yield of beta-carotene to a considerable level (98+/-2mg/l) as compared to wild strains. Effect of different media components such as carbon, nitrogen, and sulphates, and that of process variables such as pH and inoculum size on beta-carotene production by Blakeslea trispora in shake flask culture was investigated. One factor at-a-time method was employed for the optimization of media components. Response surface methodology (RSM) was further used to determine the optimum values of process variables for maximum beta-carotene production. The fit of the quadratic model was found to be significant. A significant increase in beta-carotene production (139+/-1mg/l) was achieved using RSM.
Choudhari
Sheetal
S
Food Engineering and Technology Department, Institute of Chemical Technology, University of Mumbai, Matunga, Mumbai 400 019, India. sheetalchoudhari@yahoo.co.in
Singhal
Rekha
R
D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D003198:Computer Simulation; D003470:Culture Media / Q000378:metabolism; D003627:Data Interpretation, Statistical; D008954:Models, Biological; D015233:Models, Statistical; D009090:Mucorales / Q000254:growth & development / Q000378:metabolism*; D011786:Quality Control; D019207:beta Carotene / Q000302:isolation & purification* / Q000378:metabolism*
D003470:Culture Media; D019207:beta Carotene