title: Surveillance for reassortant virus by multiplex reverse transcription-PCR specific for eight genomic segments of avian influenza A H5N1 viruses.
abstract: Avian influenza H5N1 virus is a global threat. An emergence of a reassortant virus with a pandemic potential is a major concern. Here we describe a multiplex reverse transcription-PCR assay that is specific for the eight genomic segments of the currently circulating H5N1 viruses to facilitate surveillance for a virus resulting from reassortment between human influenza virus and the H5N1 virus.
authors: Department of Microbiology, Faculty of Medicine, Siriraj Hospital, Mahidol University, 2 Prannok Road, Bangkok Noi, Bangkok 10700, Thailand.
authors: Prasert
authors: P
authors: Auewarakul
authors: Kantima
authors: K
authors: Sangsiriwut
authors: Kridsda
authors: K
authors: Chaichoune
authors: Arunee
authors: A
authors: Thitithanyanont
authors: Witthawat
authors: W
authors: Wiriyarat
authors: Taweesak
authors: T
authors: Songserm
authors: Rasameepen
authors: R
authors: Ponak-nguen
authors: Jarunee
authors: J
authors: Prasertsopon
authors: Phisanu
authors: P
authors: Pooruk
authors: Pathom
authors: P
authors: Sawanpanyalert
authors: Parntep
authors: P
authors: Ratanakorn
authors: Pilaipan
authors: P
authors: Puthavathana
mesh_terms: D016679:Genome, Viral; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics* / Q000302:isolation & purification*; D007251:Influenza, Human / Q000453:epidemiology / Q000821:virology; D011159:Population Surveillance / Q000379:methods; D016865:Reassortant Viruses / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*