[The correlation between intracellular level of nitric oxide and frequency of gene somatic mutations after low dose radiation exposure].
The purpose of this work was the study of possible relationship between intracellular NO level and somatic mutagenesis after irradiation with low doses. The level of NO in peripheral blood lymphocytes and frequency of the TCR-mutant cells were assessed by flow cytometry in 64 workers of atomic industry with mean dose (+/- SE) 114.9 +/- 10.8 MSV, accumulated within 21.4 +/- 1.1 years, and 66 age- matched control donors. The mean frequency of the TCR-mutant cells in this groups was (6.1 +/- 1.0) x 10(-4) and (4.1 +/- 0.2) x 10(-4) respectively (p = 0.06). 14% of workers of atomic industry had the TCR-mutant cell frequencies exceeding the 95% confidence interval in control donors. It was found the positive correlation between the intracellular NO level and the TCR-mutant frequency (R = 0.36, p < 0.01). The mean level of NO in individuals with the elevated TCR-mutant frequency was significantly higher than in others: 1619 +/- 57 vs 1340 +/- 40 relative units (p = 0.01). The results suggest that nitric oxide may come into elevating frequency of the mutant cells in some proportion of individuals exposed to low doses of ionizing radiation not excepting formation of genome instability.
Zamulaieva
I A
IA
Orlova
N V
NV
Smirnova
S G
SG
Proskuriakov
S Ia
SIa
Saenko
A S
AS
D004307:Dose-Response Relationship, Radiation; D005434:Flow Cytometry; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry / Q000166:cytology / Q000528:radiation effects; D008875:Middle Aged; D016296:Mutagenesis; D009154:Mutation; D009569:Nitric Oxide / Q000032:analysis / Q000378:metabolism*; D016273:Occupational Exposure; D011210:Power Plants; D011829:Radiation Dosage; D011839:Radiation, Ionizing; D011948:Receptors, Antigen, T-Cell / Q000235:genetics
D011948:Receptors, Antigen, T-Cell; D009569:Nitric Oxide