Determination of gestagens in kidney fat by liquid chromatography tandem mass spectrometry.
The use of gestagens in animal fattening is prohibited within the European Union. Recently, the use of spectrometric methods for the detection and confirmation of banned substances was made obligatory. Therefore, conventional high-performance liquid chromatographic (HPLC) methods have been superseded. It has been possible to couple a previously described HPLC method for the determination of acetyl-gestagens in kidney fat to tandem mass spectrometry (LC-MS/MS). The decision limits CCalpha and the detection capability CCbeta are found to be below the minimum required performance limit (MRPL) established for medroxyprogesterone acetate (MPA) at 1 microg kg(-1). The calculated values for CCalpha are as follows: megestrol acetate (MGA)--0.15 microg kg(-1), melengesterol acetate (MLA)--0.15 microg kg(-1), chlormadinone acetate (CMA)--0.37 microg kg(-1) and for medroxyprogesterone acetate (MPA)--0.24 microg kg(-1). The CCbeta values for these compounds have been determined as 0.19, 0.19, 0.47 and 0.32 microg kg(-1), respectively.
Lõhmus
Madis
M
Department of Chromatography, Tallinn Laboratory, Estonian Veterinary and Food Laboratory, Väike-Paala 3, 11415 Tallinn, Estonia. madis.lohmus@vetlab.ee
Kender
Tiia
T
D002138:Calibration; D002625:Chemistry, Organic / Q000379:methods; D002715:Chlormadinone Acetate / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods*; D017258:Medroxyprogesterone Acetate / Q000032:analysis; D019290:Megestrol Acetate / Q000032:analysis; D008552:Melengestrol Acetate / Q000032:analysis; D011372:Progestins / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry
D011372:Progestins; D012997:Solvents; D002715:Chlormadinone Acetate; D008552:Melengestrol Acetate; D017258:Medroxyprogesterone Acetate; D019290:Megestrol Acetate