Testing oligothiophene fluorophores under physiological conditions. Preparation and optical characterization of the conjugates of bovine serum albumin with oligothiophene N-hydroxysuccinimidyl esters.
Bovine serum albumin (BSA) was reacted with linear and newly synthesized branched oligothiophene N-hydroxysuccinimidyl ester fluorophores (TSEs) in moderately basic carbonate buffer solution. Optically stable BSA-TSE conjugates were obtained with a degree of labeling depending on experimental conditions. Conjugates with high fluorophore to BSA ratios (F/BSA = 8) displayed fluorescence quantum yields in the range of 10-30% in water at pH = 7.2, comparable to the quantum yield (25%) of the BSA-FITC conjugate prepared under the same conditions and with the same degree of labeling.
Zambianchi
Massimo
M
Istituto per la Sintesi Organica e la Fotoreattività, Consiglio Nazionale delle Ricerche, Bologna, Italy.
Barbieri
Andrea
A
Ventola
Alfredo
A
Favaretto
Laura
L
Bettini
Cristian
C
Galeotti
Matteo
M
Barbarella
Giovanna
G
D000818:Animals; D002417:Cattle; D004952:Esters / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D008163:Luminescent Measurements; D055095:Optics and Photonics; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013388:Succinimides / Q000737:chemistry*; D013876:Thiophenes / Q000737:chemistry*
D004952:Esters; D005456:Fluorescent Dyes; D013388:Succinimides; D013876:Thiophenes; D012710:Serum Albumin, Bovine