title: Distribution of capsular and surface polysaccharide serotypes of Staphylococcus aureus.
abstract: Because of its ability to cause serious and fatal infections, Staphylococcus aureus remains one of the most feared microorganisms. Methicillin-resistant S. aureus (MRSA) has long been a common pathogen in healthcare facilities, but within the past decade, it has emerged as a problematic pathogen in the community setting as well. The severe consequences of infection heighten the importance of prevention. To analyze the potential applicability of a putative S. aureus polysaccharide conjugate vaccine, we tested 714 German methicillin-susceptible S. aureus (MSSA) and MRSA strains for their capsular and surface polysaccharide serotype by slide agglutination with specific antibodies (anti-T5-DT, anti-T8-DT, anti-336-rEPA). The strain serotypes were confirmed by immunodiffusion using lysostaphin-digested cell lysates. Regarding MRSA strains representing 86 unique spa types and thus covering >90% of MRSA spa types registered, 39 (45.3%) were type 5, 36 (41.9%) were type 8, and 11 (12.8%) were type 336. Of particular interest, type 336 was the second most common serotype among MRSA isolates collected from 10 different laboratories (40 isolates per site) covering university hospitals, general hospitals, and clinics throughout Germany. Type 8-positive strains were more prevalent among isolates recovered from anterior nares of patients who did not subsequently develop S. aureus bacteremia compared with those who became bacteremic with this pathogen. In conclusion, the addition of the newly described type 336 to a capsular polysaccharide-protein conjugate vaccine could extend the coverage substantially and would include virtually all MSSA and MRSA strains currently circulating in Germany.
authors: Institute of Medical Microbiology, University Hospital of Münster, 48149 Münster, Germany. eiffc@uni-muenster.de
authors: Christof
authors: C
authors: von Eiff
authors: Kimberly L
authors: KL
authors: Taylor
authors: Alexander
authors: A
authors: Mellmann
authors: Ali I
authors: AI
authors: Fattom
authors: Alexander W
authors: AW
authors: Friedrich
authors: Georg
authors: G
authors: Peters
authors: Karsten
authors: K
authors: Becker
mesh_terms: D000372:Agglutination Tests; D005858:Germany; D006761:Hospitals; D006801:Humans; D005779:Immunodiffusion; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D012703:Serotyping; D013203:Staphylococcal Infections / Q000276:immunology / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000145:classification* / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D011135:Polysaccharides, Bacterial