Targeting neural circuitry in zebrafish using GAL4 enhancer trapping.
We present a pilot enhancer trap screen using GAL4 to drive expression of upstream activator sequence (UAS)-linked transgenes in expression patterns dictated by endogenous enhancers in zebrafish. The patterns presented include expression in small subsets of neurons throughout the larval brain, which in some cases persist into adult. Through targeted photoconversion of UAS-driven Kaede and variegated expression of UAS-driven GFP in single cells, we begin to characterize the cellular components of labeled circuits.
Scott
Ethan K
EK
Department of Physiology, University of California, San Francisco, California 94158, USA.
Mason
Lindsay
L
Arrenberg
Aristides B
AB
Ziv
Limor
L
Gosse
Nathan J
NJ
Xiao
Tong
T
Chi
Neil C
NC
Asakawa
Kazuhide
K
Kawakami
Koichi
K
Baier
Herwig
H
D000818:Animals; D030801:Animals, Genetically Modified; D001921:Brain / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D004268:DNA-Binding Proteins; D004742:Enhancer Elements, Genetic; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D009474:Neurons / Q000378:metabolism; D010865:Pilot Projects; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000235:genetics*; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*; D019076:Transgenes; D015027:Zebrafish / Q000196:embryology / Q000235:genetics* / Q000378:metabolism
D004268:DNA-Binding Proteins; C052130:GAL4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors