title: Study of the reactive dendritic cells in small B-cell lymphoproliferations of the skin.
abstract: Distinguishing between low-grade primary cutaneous B-cell lymphoma (LG-pCBCL) and cutaneous lymphoid hyperplasia (CLH) based on histological features is often difficult. CLH lesions contain numerous reactive cells of the histiocyte lineage [Langerhans cells (LC), dermal dendritic cells (DDC), and macrophages], which are also often present in CBCL. The aim of this study was to determine whether immunohistochemical detection of those cells could help differentiate between CLH and LG-pCBCL. We determined the percentages of those histiocytic cells in the dermal infiltrates of 45 cases of cutaneous lymphoproliferations comprising 16 CLH and 29 LG-pCBCL (19 follicle-center cell lymphomas and 10 marginal zone lymphomas) by immunohistochemical labeling with antibodies to CD1a, FXIIIa, and CD68 to respectively detect LC, DDC, and macrophages. To avoid observer-dependent bias, an automated morphometric analysis method was used to recognize immunoreactive cells and calculate their percentages within the infiltrate. FXIIIa(+) cells were significatively more frequent in CLH than in LG-pCBCL, whereas CD1a(+) and CD68(+) cell frequencies were comparable in the two groups. The results of our study suggest that DDC might play an important role in the genesis of cutaneous lymphomas.
authors: Département de Pathologie, Hôpital Henri Mondor, VI AP-HP, Université Paris XII, Créteil, France.
authors: Amélie
authors: A
authors: Carbonnelle
authors: Nadine
authors: N
authors: Martin-Garcia
authors: Nicolas
authors: N
authors: Ortonne
authors: Liliane
authors: L
authors: Laroche
authors: Martine
authors: M
authors: Bagot
authors: Valérie
authors: V
authors: Molinier-Frenkel
authors: Janine
authors: J
authors: Wechsler
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000032:analysis; D018949:Antigens, CD1 / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D003713:Dendritic Cells / Q000737:chemistry / Q000473:pathology*; D003937:Diagnosis, Differential; D026122:Factor XIIIa / Q000032:analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016393:Lymphoma, B-Cell / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D019310:Pseudolymphoma / Q000378:metabolism / Q000473:pathology; D012871:Skin Diseases / Q000378:metabolism / Q000473:pathology; D012878:Skin Neoplasms / Q000378:metabolism / Q000473:pathology*; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D018949:Antigens, CD1; D015214:Antigens, Differentiation, Myelomonocytic; D026122:Factor XIIIa; C419393:CD1a antigen; C067980:CD68 antigen, human; D000097382:CD68 Molecule