title: Expression of caveolin-1 in rat Leydig cells.
abstract: Caveolin-1, the first member of caveolin family reported, is recognized as the structural component of caveola, a plasma membrane invagination or vesicles that are a subcompartment distinct from clathrin-coated pits. This protein is also known to be involved in cholesterol trafficking. The aim of this study was to determine the expression of caveolin-1 in adult rat Leydig cells. Testis sections incubated with an antibody to caveolin-1 showed, by immunohistochemistry, a moderate number of Leydig cells with different degrees of immunoreaction and a strong reaction in endothelial cells and in the lamina propia of seminiferous tubules. Caveolin- 1 was detected in the cell cytoplasm with a granular pattern and on the cell surface of Leydig cells cultured 24 h on uncoated, laminin-1 or type IV collagen coated coverslips. We also observed a milder reaction in 3 h cultures. Immunoreaction was also detected in Leydig cells with an antibody to tyrosine-phosphorylated caveolin-1. By double immunofluorescent technique, we observed co-localization of caveolin- I and 313-hydroxysteroid dehydrogenase. Western blot analysis revealed a band of about 22 kDa molecular weight that was recognized with both caveolin-1 and tyrosine-phosphocaveolin-1 antibodies. Caveolin-l is one of a few proteins with a demonstrated ability to bind cholesterol in vivo. In this context, the presence of caveolin- in Leydig cells may be related to cholesterol traffic--a rate limiting step in steroid biosynthesis.
authors: Centro de Investigaciones en Reproducción, Facultad de Medicina, Universidad de Buenos Aires. Paraguay 2155, piso 10, C1121ABG Buenos Aires, Argentina.
authors: Marta B
authors: MB
authors: Casanova
authors: Livia
authors: L
authors: Lustig
authors: Emilce S
authors: ES
authors: Diaz
authors: Eliana H
authors: EH
authors: Pellizzari
authors: Selva B
authors: SB
authors: Cigorraga
authors: Berta
authors: B
authors: Denduchis
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000032:analysis; D000818:Animals; D015153:Blotting, Western; D051242:Caveolin 1 / Q000032:analysis* / Q000378:metabolism; D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism; D003593:Cytoplasm; D007985:Leydig Cells / Q000737:chemistry* / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013737:Testis / Q000166:cytology
chemical_list: D051242:Caveolin 1; D002784:Cholesterol; D015096:3-Hydroxysteroid Dehydrogenases