title: Multiple high-throughput analyses monitor the response of E. coli to perturbations.
abstract: Analysis of cellular components at multiple levels of biological information can provide valuable functional insights. We performed multiple high-throughput measurements to study the response of Escherichia coli cells to genetic and environmental perturbations. Analysis of metabolic enzyme gene disruptants revealed unexpectedly small changes in messenger RNA and proteins for most disruptants. Overall, metabolite levels were also stable, reflecting the rerouting of fluxes in the metabolic network. In contrast, E. coli actively regulated enzyme levels to maintain a stable metabolic state in response to changes in growth rate. E. coli thus seems to use complementary strategies that result in a metabolic network robust against perturbations.
authors: Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
authors: Nobuyoshi
authors: N
authors: Ishii
authors: Kenji
authors: K
authors: Nakahigashi
authors: Tomoya
authors: T
authors: Baba
authors: Martin
authors: M
authors: Robert
authors: Tomoyoshi
authors: T
authors: Soga
authors: Akio
authors: A
authors: Kanai
authors: Takashi
authors: T
authors: Hirasawa
authors: Miki
authors: M
authors: Naba
authors: Kenta
authors: K
authors: Hirai
authors: Aminul
authors: A
authors: Hoque
authors: Pei Yee
authors: PY
authors: Ho
authors: Yuji
authors: Y
authors: Kakazu
authors: Kaori
authors: K
authors: Sugawara
authors: Saori
authors: S
authors: Igarashi
authors: Satoshi
authors: S
authors: Harada
authors: Takeshi
authors: T
authors: Masuda
authors: Naoyuki
authors: N
authors: Sugiyama
authors: Takashi
authors: T
authors: Togashi
authors: Miki
authors: M
authors: Hasegawa
authors: Yuki
authors: Y
authors: Takai
authors: Katsuyuki
authors: K
authors: Yugi
authors: Kazuharu
authors: K
authors: Arakawa
authors: Nayuta
authors: N
authors: Iwata
authors: Yoshihiro
authors: Y
authors: Toya
authors: Yoichi
authors: Y
authors: Nakayama
authors: Takaaki
authors: T
authors: Nishioka
authors: Kazuyuki
authors: K
authors: Shimizu
authors: Hirotada
authors: H
authors: Mori
authors: Masaru
authors: M
authors: Tomita
mesh_terms: D002853:Chromatography, Liquid; D019295:Computational Biology; D019075:Electrophoresis, Capillary; D015180:Electrophoresis, Gel, Two-Dimensional; D004790:Enzyme Induction; D004794:Enzyme Repression; D004798:Enzymes / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D005798:Genes, Bacterial; D013058:Mass Spectrometry; D053858:Metabolic Networks and Pathways / Q000235:genetics; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D020543:Proteome; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D049490:Systems Biology / Q000379:methods*; D014158:Transcription, Genetic
chemical_list: D004798:Enzymes; D029968:Escherichia coli Proteins; D020543:Proteome; D012333:RNA, Messenger