[Identification and phylogenetic application of unique nucleotide sequence of nad7 intron2 in Rhodiola (Crassulaceae) species].
Genetic characterization of 9 populations of Rhodiola crenulata, R. fastigiata and R. sachalinensis (Crassulaceae) species from Sichuan and Jilin Provinces of China, was investigated using the conserved primer of nad7 intron 2. All PCR products about 800 bp long were shorter than other Crassulaceae plants, which were used as molecular markers to identify the Rhodiola species. The sequence of the products indicated that total exon of 53 bp and intron of 738 bp exhibit only 9 nucleotide variations. Blasting the nad7 sequences to GenBank and the phylogenetic analysis showed that the sequence of Rhodiola species was clusted independently, and the length was smaller than all the registered sequences of higher plants. The result suggests that the Rhiodola species had a unique sequence in this gene region, which might be related to the special growth condition.
Deng
Ke-Jun
KJ
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China. dengkj@uestc.edu.cn
Yang
Zu-Jun
ZJ
Liu
Cheng
C
Zhao
Wei
W
Liu
Chang
C
Feng
Juan
J
Ren
Zheng-Long
ZL
D002681:China; D029627:Crassulaceae / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D007438:Introns / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D029771:Rhodiola / Q000145:classification* / Q000235:genetics
D004272:DNA, Mitochondrial