Proteome analysis of cerebrospinal fluid in Guillain-Barré syndrome (GBS).
We used two-dimensional difference in-gel electrophoresis (2-D-DIGE) for proteome analysis of cerebrospinal fluid (CSF) in Guillain-Barré syndrome (GBS). Spots showing >2-fold difference between GBS and controls were analysed using MALDI-TOF mass spectrometry. Proteins that were up-regulated in GBS included haptoglobin, serine/threonine kinase 10, alpha-1-antitrypsin, SNC73, alpha II spectrin, IgG kappa chain and cathepsin D preprotein, while transferrin, caldesmon, GALT, human heat shock protein 70, amyloidosis patient HL-heart-peptide 127aa and transthyretin were down-regulated. Some of these proteins are reported in CSF of GBS for the first time. Accordingly, the 2-D-DIGE technology may be useful to identify disease-specific proteins in patients with GBS.
Lehmensiek
V
V
Department of Neurology, University of Ulm, Oberer Eselsberg 45, 89081 Ulm, Germany.
Süssmuth
S D
SD
Brettschneider
J
J
Tauscher
G
G
Felk
S
S
Gillardon
F
F
Tumani
H
H
D000328:Adult; D000368:Aged; D002555:Cerebrospinal Fluid / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D020275:Guillain-Barre Syndrome / Q000134:cerebrospinal fluid*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D008875:Middle Aged; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D020543:Proteome