title: Effect of process temperature on bacterial and archaeal communities in two methanogenic bioreactors treating organic household waste.
abstract: The bacterial and archaeal community structure was examined in two methanogenic anaerobic digestion processes degrading organic household waste at mesophilic (37 degrees C) and thermophilic (55 degrees C) temperatures. Analysis of bacterial clone libraries revealed a predominance of Bacteroidetes (34% of total clones) and Chloroflexi (27%) at the mesophilic temperature. In contrast, in the thermophilic clone library, the major group of clones were affiliated with Thermotogae (61%). Within the domain Archaea, the phyla Euryarchaeota and Crenarchaeota were both represented, the latter only at the mesophilic temperature. The dominating archaeons grouped with Methanospirillum and Methanosarcina species at the mesophilic and thermophilic temperature, respectively. Generally, there was a higher frequency of different sequences at the lower temperature, suggesting a higher diversity compared to the community present at the thermophilic temperature. Furthermore, it was not only the species richness that was affected by temperature, but also the phylogenetic distribution of the microbial populations.
authors: Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden. lotta.leven@mikrob.slu.se
authors: Lotta
authors: L
authors: Levén
authors: Anders R B
authors: AR
authors: Eriksson
authors: Anna
authors: A
authors: Schnürer
mesh_terms: D001105:Archaea / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques / Q000379:methods; D019149:Bioreactors / Q000382:microbiology*; D017931:DNA Primers; D005191:Family Characteristics; D008697:Methane / Q000378:metabolism*; D009930:Organic Chemicals / Q000378:metabolism; D010802:Phylogeny; D019642:RNA, Archaeal / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity; D013696:Temperature; D018505:Waste Management / Q000592:standards*
chemical_list: D017931:DNA Primers; D009930:Organic Chemicals; D019642:RNA, Archaeal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D008697:Methane