[Research of gene expression profile of liver tissue in rat sepsis model].
OBJECTIVE
To screen differentially expressed genes of liver tissue in rat sepsis model and analyze them in terms of functions.


METHODS
Thirty male Wistar rats were randomly divided into model group and blank control group with 15 rats in each group. Cecal ligation and puncture (CLP) was used to reproduce rat sepsis model, gene expression profile microarray that contains 4 096 rat cDNA clones was used to detect the change in gene expression pattern of rat liver tissue 24 hours after CLP, then differentially expressed genes that high correlated to sepsis were screened, and the functions of these genes were analyzed by means of related computer software.


RESULTS
Compared to the controls, gene expression of 522 genes in rat sepsis model were changed 24 hours after CLP, accounting for 12.7%, among them 244 gene expression down-regulated, and 278 gene expression up-regulated.


CONCLUSIONS
Multiple organ dysfunction syndrome (MODS) induced by sepsis involves to a series of gene differential expressions, such as cell cycle and control related genes, cell apoptosis genes, immunity related genes, genes concerning energy metabolism, blood system related genes, cancer related genes, growth factor genes, acute stress reaction related genes etc. Gene microarray technique can be used to comprehensively study gene expression profile in rat sepsis model, in order to find new research objectives and gene therapy strategies for sepsis.
Li
Zhi-jun
ZJ
The First Central Hospital, Tianjin 300192, China.
Li
Yin-ping
YP
Gai
Hui-rong
HR
Xue
Yong-lai
YL
Feng
Xi-zeng
XZ
D000818:Animals; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D008099:Liver / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000378:metabolism* / Q000503:physiopathology