Using the single-compartment ratio model to calculate half-life, NT-proBNP as an example.
BACKGROUND
The single-compartment model offers a simple way to calculate the half-life of a compound if it is secreted or injected at the known rate compared with another compound whose half-life is known. This model may be easier to use than the exponential decay model. Investigators disagree on the value of the half-life of NT-proBNP, with published values ranging from 70 to 120 min. Prior studies used values from sheep, which may not be appropriate in humans. Therefore, we have re-evaluated the half-life of NT-proBNP using a single-compartment model.


METHODS
The single-compartment model allows one to evaluate the half-life of NT-proBNP using the NT-proBNP:BNP ratio and the BNP half-life. We calculated the NT-proBNP:BNP ratio from 26 subjects without cardiac abnormalities.


RESULTS
The mean ratio of the NT-proBNP to BNP was 1.24 with an SEM of 0.1. Using a half-life of 20 min for BNP, the calculated half-life for NT-proBNP would be 24.8 min.


CONCLUSIONS
The single-compartment ratio model requires neither strictly first-order decay after stimulation, nor the collection of times samples. The re-calculated half-life for NT-proBNP is 25 min for humans, which differs greatly from the current literature value of 90 min and thus its half-life is closer to that of BNP in normal subjects. Accordingly its estimated time to return to a steady-state after a disturbance is 100 min, and therefore it could be useful in the monitoring of patients over short time periods. The single-compartment ratio model is fairly robust in the presence of cross-reactivity.
Kroll
Martin H
MH
Dallas VA Medical Center, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75216, USA. martin.kroll@med.va.gov
Twomey
Patrick J
PJ
Srisawasdi
Pornpen
P
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood*; D006207:Half-Life; D006331:Heart Diseases / Q000097:blood*; D006801:Humans; D008875:Middle Aged; D008954:Models, Biological; D020097:Natriuretic Peptide, Brain / Q000097:blood*; D010446:Peptide Fragments / Q000097:blood*; D012016:Reference Values
D015415:Biomarkers; D010446:Peptide Fragments; C109794:pro-brain natriuretic peptide (1-76); D020097:Natriuretic Peptide, Brain