title: First detection of a VIM-1 metallo-beta-lactamase in a carbapenem-resistant Citrobacter freundii clinical isolate in an acute hospital in Germany.
abstract: We report the first detection of a carbapenem-resistant Citrobacter freundii clinical strain in Germany. It was isolated from an abscess of a patient with acute necrotic pancreatitis in an acute hospital. PCR and sequencing experiments revealed that the carbapenem resistance was mediated by a VIM-1 metallo-beta-lactamase, located on a plasmid encoded class 1 integron. Carbapenem resistance in Enterobacteriaceae is so far a rare event and 1 of the major therapeutic concerns in the future.
authors: Department of Clinical Chemistry and Laboratory Medicine, Robert Bosch Hospital, Stuttgart, Germany. jan.weile@ikp-stuttgart.de
authors: Jan
authors: J
authors: Weile
authors: Harald
authors: H
authors: Rahmig
authors: Sabine
authors: S
authors: Gfröer
authors: Klaus
authors: K
authors: Schroeppel
authors: Cornelius
authors: C
authors: Knabbe
authors: Milorad
authors: M
authors: Susa
mesh_terms: D000369:Aged, 80 and over; D015780:Carbapenems / Q000494:pharmacology*; D015377:Cilastatin / Q000627:therapeutic use; D002939:Ciprofloxacin / Q000627:therapeutic use; D016971:Citrobacter freundii / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000188:drug therapy / Q000382:microbiology; D005260:Female; D005858:Germany; D006801:Humans; D015378:Imipenem / Q000627:therapeutic use; D016133:Polymerase Chain Reaction / Q000379:methods; D018440:beta-Lactam Resistance / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism
chemical_list: D015780:Carbapenems; D015377:Cilastatin; D002939:Ciprofloxacin; D015378:Imipenem; C417264:VIM-1 metallo-beta-lactamase; D001618:beta-Lactamases