title: Anti-leishmania activity of semi-purified fraction of Jacaranda puberula leaves.
abstract: The crude methanolic extract from leaves of Jacaranda puberula showed activity against Leishmania (Leishmania) amazonensis. The extract presented active against promastigote forms with an inhibitory concentration 50% (IC(50)) value of 88.0 mug/ml, but only moderated activity against amastigote forms; however in higher concentrations the extract showed cytotoxic effects. The bio-guided chromatographic fractionation the crude methanolic extract against amastigotes yielded a fraction with an IC(50) value of 14.0 mug/ml (without cytotoxic activity) in relation to the crude extract (IC(50) value, 359.0 microg/ml). These data indicate that J. puberula leaves contain active compounds, which should be further investigated for the development of new potential drugs against cutaneous leishmaniasis.
authors: Departamento de Patologia, Faculdade de Medicina, Universidade de São Paulo, Av. Dr. Arnaldo 455, Cerqueira César, São Paulo 0146-903, SP, Brazil. felipepassero@yahoo.com.br
authors: L F D
authors: LF
authors: Passero
authors: A A
authors: AA
authors: Castro
authors: T Y
authors: TY
authors: Tomokane
authors: M J
authors: MJ
authors: Kato
authors: T F
authors: TF
authors: Paulinetti
authors: C E P
authors: CE
authors: Corbett
authors: M D
authors: MD
authors: Laurenti
mesh_terms: D000818:Animals; D000981:Antiprotozoal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D029402:Bignoniaceae / Q000737:chemistry*; D002478:Cells, Cultured; D007891:Leishmania / Q000187:drug effects* / Q000254:growth & development; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D021261:Parasitic Sensitivity Tests; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D018515:Plant Leaves / Q000737:chemistry*
chemical_list: D000981:Antiprotozoal Agents; D010936:Plant Extracts