title: Kinetic determination of morphine by means of Bray-Liebhafsky oscillatory reaction system using analyte pulse perturbation technique.
abstract: A novel kinetic method for micro-quantitative determinations of morphine (MH) is proposed and validated. The method is based on the potentiometric monitoring of the concentration perturbations of the oscillatory reaction system being in a stable non-equilibrium stationary state close to the bifurcation point between stable and oscillatory state. The response of the Bray-Liebhafsky (BL) oscillatory reaction as a matrix system, to the perturbations by different concentrations of morphine, is followed by a Pt-electrode. The proposed method relies on the linear relationship between maximal potential shift, DeltaE(m), and the logarithm of the added morphine amounts in the range of 0.004-0.18 micromol. Under optimum conditions, the sensitivity of the proposed method (as the limit of detection) is 0.001 micromol and the method is featured by good precision (R.S.D.=1.6%) as well as the excellent sample throughput (45 samples h(-1)). In addition to standard solution analysis, this approach was successfully applied for quantitative determination of morphine in a typical pharmaceutical dosage form. Some aspects of the possible mechanism of morphine action on the BL oscillating chemical system are discussed in detail.
authors: Faculty of Pharmacy, Department of Physical Chemistry, University of Belgrade, Vojvode Stepe 450, YU-11000 Belgrade, Serbia and Montenegro. bimesel@EUnet.yu <bimesel@EUnet.yu>
authors: Natasa D
authors: ND
authors: Pejić
authors: Slavica M
authors: SM
authors: Blagojević
authors: Slobodan R
authors: SR
authors: Anić
authors: Vladana B
authors: VB
authors: Vukojević
authors: Miroslav D
authors: MD
authors: Mijatović
authors: Jasna S
authors: JS
authors: Cirić
authors: Zoran S
authors: ZS
authors: Marković
authors: Svetlana D
authors: SD
authors: Marković
authors: Ljiljana Z
authors: LZ
authors: Kolar-Anić
mesh_terms: D002384:Catalysis; D004566:Electrodes; D007700:Kinetics; D009020:Morphine / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D009020:Morphine