Comprehensive proteomic analysis of human cervical-vaginal fluid.
Cervical-vaginal fluid (CVF) is a potential rich source of biomarkers for enhancing our understanding of human parturition and pathologic conditions affecting pregnancy. In this study, we performed a comprehensive survey of the CVF proteome in pregnancy utilizing multidimensional liquid chromatography (2D-LC) coupled with mass spectrometry and gel-electrophoresis-based protein separation and identification. In total, 150 unique proteins were identified using multiple protein identification algorithms. Metabolism (32%) and immune response-related (22%) proteins are the major functional categories represented in the CVF proteome. A comparison of the CVF, serum, and amniotic fluid proteomes showed that 77 proteins are unique to CVF, while 56 and 17 CVF proteins also occur in serum and amniotic fluid, respectively. This data set provides a foundation for evaluation of these proteins as potential CVF biomarkers for noninvasive diagnosis of pregnancy-related disorders.
Dasari
Surendra
S
Department of Pediatrics, Oregon Health and Science University, Portland, Oregon 97239, USA.
Pereira
Leonardo
L
Reddy
Ashok P
AP
Michaels
John-Edward A
JE
Lu
Xinfang
X
Jacob
Thomas
T
Thomas
Archana
A
Rodland
Matthew
M
Roberts
Charles T
CT
Gravett
Michael G
MG
Nagalla
Srinivasa R
SR
D000653:Amniotic Fluid / Q000737:chemistry; D015415:Biomarkers / Q000032:analysis; D001826:Body Fluids / Q000737:chemistry; D002584:Cervix Uteri / Q000737:chemistry*; D002853:Chromatography, Liquid; D005260:Female; D006801:Humans; D011247:Pregnancy; D011248:Pregnancy Complications / Q000175:diagnosis*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D014621:Vagina / Q000737:chemistry*
D015415:Biomarkers; D020543:Proteome