title: A novel type-1 cytokine receptor from fish involved in the Janus kinase/signal transducers and activators of transcription (Jak/STAT) signal pathway.
abstract: Type I cytokine receptors mediate the action of the members of the long chain cytokines canonically involved in numerous physiological function. Here we report a novel cytokine receptor termed Japanese flounder glycoprotein 130 homologue or JfGPH, exhibiting the unique type I cytokine receptor motifs i.e. having a cytokine binding domain (CBD) containing two pairs of conserved cysteine (C) residues, a WSXWS motif, three fibronectin domains all in the extracellular region. It is also composed of the Jak binding domains Box 1 and Box 2, and a STAT 3 binding motif (Box 3) in the cytoplasmic region suggesting its mediatory role for Janus kinase/Signal transducers and activators of transcription (Jak/STAT) signal pathway. The JfGPH cDNA is about 3kb encoding 801 amino acid residues with a predicted molecular weight of 90kDa and its gene has an 11-exon/10-intron architecture. While JfGPH shows significant homology with the members of type-1 cytokine receptor family including IL6ST (or gp130), IL31alpha (or GLMR), CSF3R (or GCSFR), LIFR, OSMR, IL12Rbeta1 and LEPR, structural and phylogenetic analysis of its protein revealed that it is a novel and an ancestral cytokine receptor found in teleost. JfGPH gene is ubiquitously expressed in Japanese flounder tissues and in a natural embryo (HINAE) cell line showing its critical role in teleost physiological functions similar to gp130 in higher vertebrates. High expression of JfGPH transcripts in immune-related tissues and, in ovary and embryo-derived cell line suggest its role in immune responses, and reproduction/development, respectively. In vitro stimulation of spleen, kidney, peripheral blood leukocytes (PBLs) and HINAE revealed that JfGPH is down-regulated by polyinosinic:polycytidylic acid (poly I:C), an interferon (IFN) inducer, suggesting an apparent control of the JfGPH's expression during IFN-induced Jak/STAT signaling.
authors: Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Konan 4-5-7 Minato-ku, Tokyo 108-8477, Japan.
authors: Mudjekeewis D
authors: MD
authors: Santos
authors: Motoshige
authors: M
authors: Yasuike
authors: Hidehiro
authors: H
authors: Kondo
authors: Ikuo
authors: I
authors: Hirono
authors: Takashi
authors: T
authors: Aoki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D050822:Cytokine Receptor gp130 / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D004622:Embryo, Mammalian; D004625:Embryo, Nonmammalian; D005412:Flatfishes; D006801:Humans; D053612:Janus Kinases / Q000502:physiology*; D008969:Molecular Sequence Data; D050791:STAT Transcription Factors / Q000502:physiology*; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000235:genetics
chemical_list: D050791:STAT Transcription Factors; D050822:Cytokine Receptor gp130; D053612:Janus Kinases