Feasibility of bone marrow stromal cells autologous transplantation for dilated cardiomyopathy.
The feasibility of bone marrow stromal cells autologous transplantation for rabbit model of dilated cardiomyopathy induced by adriamycin was studied. Twenty rabbits received 2 mg/kg of adriamycin intravenously once a week for 8 weeks (total dose, 16 mg/kg) to induce the cardiomyopathy model with the monitoring of cardiac function by transthoracic echocardiography. Marrow stromal cells were isolated from cell-transplanted group rabbits and were culture-expanded on the 8th week. On the 10th week, cells were labeled with 4,6-diamidino-2-phenylindole (DAPI), and then injected into the myocardium of the same rabbits. The results showed that viable cells labeled with DAPI could be identified in myocardium at 2nd week after transplantation. Histological findings showed the injury of the myocardium around the injection site was relieved with less apoptosis and more expression of bcl-2. The echocardiography found the improvement of local tissue movement from (2.12+/-0.51) cm/s to (3.81+/-0.47) cm/s (P<0.05) around the inject site, but no improvement of heart function as whole. It was concluded bone marrow stromal cells transplantation for dilated cardiomyopathy was feasibe. The management of cells in vitro, the quantity and the pattern of the cells transplantation and the action mechanism still need further research.
Zhou
Cheng
C
Department of Cardiosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. zhoucheng_xhxw@yahoo.com.cn
Yang
Chenyuan
C
Xiao
Shiliang
S
Fei
Hongwen
H
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity; D017209:Apoptosis / Q000502:physiology; D016026:Bone Marrow Transplantation; D002311:Cardiomyopathy, Dilated / Q000139:chemically induced / Q000503:physiopathology / Q000601:surgery*; D002469:Cell Separation; D002478:Cells, Cultured; D004317:Doxorubicin / Q000633:toxicity; D004452:Echocardiography; D005240:Feasibility Studies; D005456:Fluorescent Dyes / Q000378:metabolism; D020287:In Situ Nick-End Labeling / Q000379:methods; D007211:Indoles / Q000378:metabolism; D009206:Myocardium / Q000166:cytology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D011817:Rabbits; D011897:Random Allocation; D017154:Stromal Cells / Q000378:metabolism / Q000502:physiology / Q000637:transplantation; D013997:Time Factors; D014182:Transplantation, Autologous; D016277:Ventricular Function, Left
D000903:Antibiotics, Antineoplastic; D005456:Fluorescent Dyes; D007211:Indoles; D019253:Proto-Oncogene Proteins c-bcl-2; C007293:DAPI; D004317:Doxorubicin