title: Sinomenine inhibits primary CD4+ T-cell proliferation via apoptosis.
abstract: Sinomenine is an active component isolated from Sinomenium acutum and is widely used as an immunosuppressive drug for treating autoimmune diseases. CD4(+) T-cell population plays a key role in adaptive immune response and is related to some autoimmune diseases. In this study, we investigated the possible immunosuppressive effect of sinomenine on CD4(+) T cells and its underlying mechanism. Our data demonstrated that sinomenine remarkably suppressed the proliferation of CD4(+) T cells, blocked the cell cycle progression from G0/G1 phase to S plusG2/M phases. Finally, the immunosuppressive activity elicited by sinomenine in CD4(+) primary lymphocytes was found to be largely accounted for by caspase 3-dependent cells apoptosis. Sinomenine did not significantly alter the expression of bcl-2 in activated CD4(+) primary T cells, suggesting that bcl-2 might not be involved in sinomenine-induced T cells apoptosis. In sum, this study proposes a novel mechanism for the immunosuppressive function of sinomenine on primary mouse CD4(+) T cells.
authors: State Key Laboratory of Pharmaceutical Biotechnology, Department of Biochemistry, College of Life Sciences, Nanjing University, 22 Hankou Road, Nanjing 210093, P.R. China.
authors: Luan
authors: L
authors: Shu
authors: Wu
authors: W
authors: Yin
authors: Jing
authors: J
authors: Zhang
authors: Bo
authors: B
authors: Tang
authors: Yuan-Xi
authors: YX
authors: Kang
authors: Fan
authors: F
authors: Ding
authors: Zi-Chun
authors: ZC
authors: Hua
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009019:Morphinans / Q000494:pharmacology*
chemical_list: D007166:Immunosuppressive Agents; D009019:Morphinans; C009271:sinomenine