Analytical method for localizing a fluorescent inclusion in a turbid medium.
We describe a novel method for localizing a fluorescent inclusion in a homogeneous turbid medium through the use of time-resolved techniques. Based on the calculation of the mean time of the fluorescence curves, the method does not require a priori knowledge of either the fluorescence lifetime or the mean time of the instrument response function since it adopts a differential processing approach. Theoretical expressions were validated and experiments for assessing the accuracy of localization were carried out on liquid optical phantoms with a small fluorescent inclusion. The illumination and detection optical fibers were immersed in the medium to achieve infinite medium geometry as required by the model used. The experimental setup consisted of a time-correlated single-photon counting system. Submillimeter accuracy was achieved for the localization of the inclusion.
Laidevant
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A
Département micro Technologies pour la Biologie et la Santé, CEA-LETI Recherche Technologique Grenoble, Grenoble, France. aurelie.laidevant@wanadoo.fr
Da Silva
A
A
Berger
M
M
Boutet
J
J
Dinten
J-M
JM
Boccara
A C
AC
D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D053758:Nanoparticles / Q000648:ultrastructure*; D009391:Nephelometry and Turbidimetry / Q000295:instrumentation* / Q000379:methods; D010316:Particle Size; D019047:Phantoms, Imaging