Relationship between contents of lipocalin-type prostaglandin D synthase on the surface of infertility sperm and in seminal plasma.
Lipocalin-type prostaglandin D synthase (L-PGDS) is localized in Leydig cells, sperm, and epithelial cells of the epididymis. The present study was to determine the correlation between content of this enzyme in seminal plasma and on the surface of sperm. We analyzed 90 semen samples. L-PGDS in seminal plasma was analyzed by an ELISA procedure. L-PGDS on sperm was analyzed by flow cytometry. The semen donors were categorized in three groups: normal, oligospermic, and azoospermic. According to results obtained, L-PGDS may have the ability to improve progressive motility of sperm, and L-PGDS in seminal plasma and on sperm surface may impact male fertility in the female reproductive tract.
Chen
De-Yu
DY
Research Center for Reproductive Medicine, Department of Cell Biology and Genetics, Shantou University Medical College, Shantou, Guangdong, China.
Zhu
Mao-Ying
MY
Cui
Ya-Dong
YD
Huang
Tian-Hua
TH
D005434:Flow Cytometry; D006801:Humans; D007248:Infertility, Male / Q000201:enzymology*; D019746:Intramolecular Oxidoreductases / Q000378:metabolism*; D054834:Lipocalins; D008297:Male; D012661:Semen / Q000201:enzymology*; D013094:Spermatozoa / Q000201:enzymology*
D054834:Lipocalins; D019746:Intramolecular Oxidoreductases; C022466:prostaglandin R2 D-isomerase