title: Interaction of DNA oligomers with cationic lipidic monolayers: complexation and splitting.
abstract: Interactions of native DNA with octadecylamine (ODA) and hexadecymdimethylammonium bromide (HTAB) monolayers at the air/water interface were studied by pi-A isotherms, ellipsometry, and X-ray reflectivity. We show that the microscopic structure of ODA-DNA complexes is definitely consistent with a single-stranded form for DNA. On the contrary, with HTAB, DNA complexes in its native form. The crucial difference in the behavior of these two fairly similar lipids is due to the presence of the amine group in ODA. These results should be relevant to applications such as DNA chips and sensors.
authors: Department of Physics, University of Parma, Viale Usberti 7 A, 43100 Parma, Italy. erokhin@fis.unipr.it
authors: Svetlana
authors: S
authors: Erokhina
authors: Tatiana
authors: T
authors: Berzina
authors: Luigi
authors: L
authors: Cristofolini
authors: Oleg
authors: O
authors: Konovalov
authors: Victor
authors: V
authors: Erokhin
authors: Marco P
authors: MP
authors: Fontana
mesh_terms: D000327:Adsorption; D000388:Air; D000588:Amines / Q000737:chemistry; D002412:Cations; D002627:Chemistry, Physical / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry*; D008958:Models, Molecular; D009841:Oligonucleotides / Q000737:chemistry*; D011312:Pressure; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D013499:Surface Properties; D014867:Water / Q000737:chemistry; D014965:X-Rays
chemical_list: D000588:Amines; D002412:Cations; D008055:Lipids; D009841:Oligonucleotides; D000644:Quaternary Ammonium Compounds; D014867:Water; D004247:DNA; C009317:stearylamine; C027118:didecyldimethylammonium