title: A kinetic method amenable to automation for ceruloplasmin estimation with inexpensive and stable reagents.
abstract: OBJECTIVE
  To develop a kinetic, inexpensive, automatable assay for ceruloplasmin as ferroxidase.
  
  
  METHODS
  Dithiothreitol has been used to stabilize the substrate and quinolones to complex the ferric.
  
  
  RESULTS
  Mean ferroxidase activity in healthy adults, Wilson's disease and pregnancy was 787.29, 178.5 and 1828.09 IU/L, respectively. Correlation coefficient of ferroxidase vis-à-vis copper, ferroxidase by ferrozine and ceruloplasmin were 0.90, 0.94 and 0.92, respectively.
  
  
  CONCLUSIONS
  Norfloxacin method is a simple, inexpensive and automatable kinetic assay.
authors: Department of Biochemistry, Armed Forces Medical College, Pune-411040, India. blsomani@gmail.com
authors: Babu Lal
authors: BL
authors: Somani
authors: Vivek
authors: V
authors: Ambade
mesh_terms: D000328:Adult; D002570:Ceruloplasmin / Q000032:analysis* / Q000378:metabolism*; D019963:Clinical Chemistry Tests / Q000191:economics / Q000379:methods*; D004229:Dithiothreitol / Q000737:chemistry; D005260:Female; D005297:Ferrozine / Q000737:chemistry; D006527:Hepatolenticular Degeneration / Q000201:enzymology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D009643:Norfloxacin / Q000737:chemistry; D011247:Pregnancy; D015363:Quinolones / Q000737:chemistry; D015203:Reproducibility of Results
chemical_list: D015363:Quinolones; D005297:Ferrozine; D002570:Ceruloplasmin; D009643:Norfloxacin; D004229:Dithiothreitol