[Enzymatic depolymerization of N-succinylchitosan].
A complex of chitinolytic enzymes of Streptomyces kurssanovii and also lysozyme and Celloviridin, an industrial cellulase preparation, were demonstrated to provide for an enzymatic hydrolysis of N-succinylchitosan. Our studies were carried out on a high-molecular N-succinylchitosan with M of 390 kDa and a substitution degree of 0.8 as a substrate. All the enzymatic preparations were shown to be suitable for the obtaining of low-molecular derivatives of N-succinylchitosan. The complex of enzymes from S. kurssanovii showed the greatest activity: they reduced the characteristic viscosity of initial solution of the substrate by 78% for 30 min. A biodegradation of N-succinylchitosan of various molecular masses was shown to proceed under the action of lysozyme, and the cleavage reaction was revealed to decelerate at a decrease in the polymer molecular mass. A use of N-succinylchitosan in a complex with drugs for a prolongation of their action in a live organism was presumed.
Il'ina
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Varlamov
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D002480:Cellulase / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D004798:Enzymes / Q000737:chemistry*; D006868:Hydrolysis; D009113:Muramidase / Q000737:chemistry; D013302:Streptomyces / Q000201:enzymology
D004798:Enzymes; C118639:N-succinyl-chitosan; D048271:Chitosan; D009113:Muramidase; D002480:Cellulase