Evaluation of extracellular products and mutagenicity in cyanobacteria cultures separated from a eutrophic reservoir.
The algal extracellular products (ECPs) in three cultures of cyanobacteria species (Anabaena, Microcystis, and Oscillatoria) dominating the eutrophic reservoir populations and their toxins have been investigated in the present work. Using gas chromatography coupled with high-resolution electron-impact mass spectrometry (GC/EI-MS) and high performance anion-exchange chromatography (HPAEC) techniques, more than 20 compounds were found in the algal culture (including cells and filtrates) extracts. The main identified ECPs were classified to polysaccharides, hydrocarbons, and aldehydes. Odor causing substances such as trans-1,10-dimethyl-trans-9-decalol (geosmin) and 2-methylisoborneol (2-MIB)were also found in the algal cultures. The potential mutagenicity of the algal suspensions was also studied with the Ames test. The organic extracts of the algal suspension from the axenic cultures were mutagenicity in TA98 without S9 mix and in TA100 with and without S9 mix. The results indicate that the ECPs of three algae species dominating the eutrophic reservoir were mutagenic clearly in the bacterial test.
Huang
Winn-Jung
WJ
Department of Environmental Engineering, Hung Kuang University, 34 Chung Chie Road, Sha-Lu, Taichung, Taiwan, ROC. huangwj@sunrise.hk.edu.tw <huangwj@sunrise.hk.edu.tw>
Lai
Chun-Hsi
CH
Cheng
Yung-Ling
YL
D001892:Camphanes / Q000032:analysis; D002244:Carbon / Q000032:analysis; D002264:Carboxylic Acids / Q000032:analysis; D002734:Chlorophyll / Q000032:analysis; D000077194:Chlorophyll A; D045424:Complex Mixtures / Q000633:toxicity*; D000458:Cyanobacteria / Q000737:chemistry*; D005068:Eutrophication; D006838:Hydrocarbons / Q000032:analysis; D008387:Marine Toxins; D052998:Microcystins / Q000032:analysis; D009152:Mutagenicity Tests; D009284:Naphthols / Q000032:analysis; D011134:Polysaccharides / Q000032:analysis; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics; D013624:Taiwan; D014881:Water Supply
D001892:Camphanes; D002264:Carboxylic Acids; D045424:Complex Mixtures; D006838:Hydrocarbons; D008387:Marine Toxins; D052998:Microcystins; D009284:Naphthols; D011134:Polysaccharides; D002734:Chlorophyll; C005536:2-methylisoborneol; D002244:Carbon; C057862:cyanoginosin LR; C001278:geosmin; D000077194:Chlorophyll A