The secreted signaling protein factor C triggers the A-factor response regulon in Streptomyces griseus: overlapping signaling routes.
Members of the prokaryotic genus Streptomyces produce over 60% of all known antibiotics and a wide range of industrial enzymes. A leading theme in microbiology is which signals are received and transmitted by these organisms to trigger the onset of morphological differentiation and antibiotic production. The small gamma-butyrolactone A-factor is an important autoregulatory signaling molecule in streptomycetes, and A-factor mutants are blocked in development and antibiotic production. In this study we showed that heterologous expression of the 324-amino acid secreted regulatory protein Factor C resulted in restoration of development and enhanced antibiotic production of an A-factor-deficient bald mutant of Streptomyces griseus, although the parental strain lacks an facC gene. Proteome analysis showed that in the facC transformant the production of several secreted proteins that belong to the A-factor regulon was restored. HPLC-MS/MS analysis indicated that this was due to restoration of A-factor production to wild-type levels in the transformant. This indicates a connection between two highly divergent types of signaling molecules and possible interplay between their regulatory networks.
Birkó
Zsuzsanna
Z
Department of Human Genetics, Faculty of Medicine, Medical and Health Science Center, University of Debrecen, Nagyerdei körút 98, H-4032 Debrecen, Hungary.
Bialek
Sylwia
S
Buzás
Krisztina
K
Szájli
Emília
E
Traag
Bjørn A
BA
Medzihradszky
Katalin F
KF
Rigali
Sebastien
S
Vijgenboom
Erik
E
Penyige
András
A
Kele
Zoltán
Z
van Wezel
Gilles P
GP
Biró
Sándor
S
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000235:genetics / Q000502:physiology; D001426:Bacterial Proteins / Q000502:physiology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D020543:Proteome / Q000032:analysis; D018085:Regulon; D015398:Signal Transduction; D013305:Streptomyces griseus / Q000235:genetics / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods
D001426:Bacterial Proteins; D020543:Proteome; C402566:factor C, Streptomyces; C025035:A-factor (Streptomyces); D015107:4-Butyrolactone