The protective effect of interleukin-11 on the cell death induced by X-ray irradiation in cultured intestinal epithelial cell.
Interleukin-11 (IL-11) is a well known anti-inflammatory cytokine that is associated with cell growth, and also participates in limiting X-ray irradiation induced intestinal mucosal injury. The aim of this study was to evaluate the protective effect of IL-11 on the cell injury induced by X-ray irradiation in rat intestinal epithelial IEC-18 cells. Recombinant human IL-11 (rhIL-11) treated cells were irradiated and then examined for cell viability. To evaluate irradiation injury, trypan blue staining was used to detect the dead cells. The viability of irradiated cells was up-regulated by rhIL-11 treatment and also resulted in the activation of p90 ribosomal S6 kinase (p90RSK) and S6 ribosomal protein (S6Rp). Wortmannin, a specific inhibitor of PI3K, suppressed the activation of S6Rp in rhIL-11 treated cells, and decreased the up-regulation of viability by rhIL-11 treatment in irradiated cells. The TUNEL assay was also perfomed to estimate the rate of apoptosis in X-ray induced cell death. There was no difference in the results between trypan blue staining and the TUNEL assay. Further, rhIL-11 down-regulated the expression of cleaved caspase-3 in irradiated cells. These results suggest that rhIL-11 may play an important role in protection from radiation injury.
Uemura
Takashi
T
Department of Tumor and Diagnostic Pathology, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, Japan.
Nakayama
Toshiyuki
T
Kusaba
Takafumi
T
Yakata
Yuichi
Y
Yamazumi
Kazuyuki
K
Matsuu-Matsuyama
Mutsumi
M
Shichijo
Kazuko
K
Sekine
Ichiro
I
D000818:Animals; D015153:Blotting, Western; D016923:Cell Death / Q000528:radiation effects*; D002460:Cell Line; D002470:Cell Survival; D015536:Down-Regulation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004847:Epithelial Cells / Q000528:radiation effects*; D006801:Humans; D017370:Interleukin-11 / Q000378:metabolism*; D007422:Intestines / Q000528:radiation effects*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry / Q000494:pharmacology; D014965:X-Rays
D004791:Enzyme Inhibitors; D017370:Interleukin-11; D011994:Recombinant Proteins; D019869:Phosphatidylinositol 3-Kinases