Effects of the controlled-released basic fibroblast growth factor from chitosan-gelatin microspheres on human fibroblasts cultured on a chitosan-gelatin scaffold.
To provide for prolonged, site-specific delivery of basic fibroblast growth factor (bFGF) to the grafted skin in a convenient manner, biodegradable chitosan-gelatin microspheres containing bFGF were fabricated and incorporated into a porous chitosan-gelatin scaffold in this study. The microspheres are an integral part of the porous three-dimensional scaffolds, and their incorporation does not significantly affect the scaffold porosity and the pore size. The release kinetics of bFGF showed a fast release (23.7%) at the initial phase in the first 2 days, and the ultimate accumulated release was approximately 71.8% by day 14, indicating an extended time course for complete release. Human fibroblasts seeded on chitosan-gelatin scaffolds with and without bFGF-loaded chitosan-gelatin microspheres (bFGF-MS) were incubated in vitro for 2 weeks and showed that, compared to chitosan-gelatin scaffolds alone, the scaffolds with bFGF-MS significantly augmented the proliferation and glycosaminoglycan (GAG) synthesis of human fibroblasts. Moreover, real-time reversed transcribed polymerase chain reaction (RT-PCR) analysis for fibroblast-related extracellular matrix (ECM) gene markers demonstrated that the transcript level of laminin was markedly upregulated by about 9-fold. These results suggest that chitosan-gelatin scaffolds with bFGF-MS possess a promising potential as a tissue engineering scaffold to improve skin regeneration efficacy and to promote vascularization.
Liu
Haifeng
H
Research Institute of Polymer Materials, Tianjin University, Tianjin 300072, People's Republic of China.
Fan
Hongbin
H
Cui
Yuanlu
Y
Chen
Yiping
Y
Yao
Kangde
K
Goh
James C H
JC
D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D003692:Delayed-Action Preparations; D004655:Emulsions / Q000737:chemistry; D005109:Extracellular Matrix / Q000235:genetics; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage*; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012038:Regeneration; D012867:Skin / Q000166:cytology / Q000187:drug effects; D012879:Skin Physiological Phenomena
D003692:Delayed-Action Preparations; D004655:Emulsions; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2; D005780:Gelatin; D048271:Chitosan