title: Expression of ferroportin1, hephaestin and ceruloplasmin in rat heart.
abstract: Iron-mediated injury plays an important role in a number of heart disorders. Studies on heart iron are therefore crucial for understanding the causes of excessive heart iron. Heart cells have the ability to accumulate transferrin-bound-iron via the transferrin receptor and non-transferrin-bound-iron probably via the L-type Ca2+ channel and the divalent metal transporter1. However, little is known about the mechanisms of iron export in the heart cells. Here, we investigated expression of iron exporters including ferroportin 1 (Fpn1), ceruloplasmin (CP) and hephaestin (Heph) and provided evidence for their existence in the heart. We demonstrated that iron has a significant effect on expression of Fpn1 and CP, but not Heph. Treatment of a high-iron diet induced a significant increase in Fpn1, a decrease in CP but no change in Heph mRNA and protein. The control of Fpn1 and CP protein expression by iron was parallel to that of their mRNA expression, suggesting a transcriptional regulation of Fpn1 and CP by iron. The existence of these proteins in the heart implies that they might have a role in heart iron homeostasis.
authors: Laboratory of Iron Metabolism, Department of Applied Biology and Chemical Technology and National Key Laboratory of Chinese Medicine and Molecular Pharmacology (Shenzhen), Hong Kong, PR China. bczmqian@polyu.edu.hk
authors: Zhong Ming
authors: ZM
authors: Qian
authors: Yan Zhong
authors: YZ
authors: Chang
authors: Gina
authors: G
authors: Leung
authors: Jun Rong
authors: JR
authors: Du
authors: Li
authors: L
authors: Zhu
authors: Qin
authors: Q
authors: Wang
authors: Lijian
authors: L
authors: Niu
authors: You Jia
authors: YJ
authors: Xu
authors: Lei
authors: L
authors: Yang
authors: Kwok Ping
authors: KP
authors: Ho
authors: Ya
authors: Y
authors: Ke
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D027682:Cation Transport Proteins / Q000096:biosynthesis*; D002570:Ceruloplasmin / Q000096:biosynthesis*; D007501:Iron / Q000008:administration & dosage / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis*; D009206:Myocardium / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D011990:Receptors, Transferrin / Q000096:biosynthesis; D015533:Transcriptional Activation; D000098742:Ferroportin
chemical_list: D027682:Cation Transport Proteins; C492163:Heph, protein, rat; D008565:Membrane Proteins; D012333:RNA, Messenger; D011990:Receptors, Transferrin; D000098742:Ferroportin; D007501:Iron; D002570:Ceruloplasmin