title: [Relationship between the melanocortin receptor 1 (MC1R) gene and the coat color phenotype in cattle].
abstract: The MC1R gene is related to coat color in cattle and to lactoprotein content in milk. Polymerase chain reaction-restriction fragment length polymorphism and DNA sequencing were used to analyze the MC1R of China Holstein Black-white Cattle, China Holstein Red-white Cattle, Luxi Yellow Cattle and Bohai Black Cattle. Three alleles (ED, E+ and e) were identified. Most China Holstein Black-white Cattle had ED and E+ alleles (ED = 0.12, E+ = 0.80). The same was true for Bohai Black Cattle (ED = 0.52, E+ = 0.47). On the other hand, most China Holstein Red-white Cattle and Luxi Yellow Cattle had the e allele (e/e = 0.95). E+/e genotype was present in China Holstein Red-white Cattle and Luxi Yellow Cattle. We reasoned that ED and E+ lad to the synthesis of the black pigment. Furthermore, we found an important SNP (Single Nucleotide Polymorphism) at 725 position of the MC1R coding region.
authors: Dairy Cattle Research Centre, Shandong Academy of Agricultural Science, Jinan, China. ganhaiyun_ren@163.com
authors: Hai-Yun
authors: HY
authors: Gan
authors: Jian-Bin
authors: JB
authors: Li
authors: Hong-Mei
authors: HM
authors: Wang
authors: Yun-Dong
authors: YD
authors: Gao
authors: Wen-Hao
authors: WH
authors: Liu
authors: Jing-Peng
authors: JP
authors: Li
authors: Ji-Feng
authors: JF
authors: Zhong
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D005838:Genotype; D008969:Molecular Sequence Data; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D044102:Receptor, Melanocortin, Type 1 / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D012880:Skin Pigmentation / Q000235:genetics*
chemical_list: D044102:Receptor, Melanocortin, Type 1