Detection of Campylobacter jejuni and Campylobacter coli in chicken meat samples by real-time nucleic acid sequence-based amplification with molecular beacons.
A nucleic acid sequence-based amplification (NASBA) assay based on molecular beacons was used for real-time detection of Campylobacter jejuni and Campylobacter coli in samples of chicken meat. A set of specific primers and beacon probe were designed to target the 16S rRNA of both species. The real-time NASBA protocol including the RNA isolation was valid for both of the cell suspensions in buffered saline and the artificially contaminated chicken meat samples. The presence of rRNA could be correlated with cellular viability, following inactivation of the bacteria by heating, in inoculated chicken meat samples but not in RNase-free cell suspensions.
Churruca
E
E
Departamento de Inmunología, Microbiología y Parasitología, Facultad de Farmacia, Universidad del País Vasco (UPV-EHU), Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Spain.
Girbau
C
C
Martínez
I
I
Mateo
E
E
Alonso
R
R
Fernández-Astorga
A
A
D000818:Animals; D017000:Campylobacter coli / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D016123:Campylobacter jejuni / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D002645:Chickens; D015342:DNA Probes; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D005784:Gene Amplification; D008460:Meat / Q000382:microbiology*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012680:Sensitivity and Specificity; D013045:Species Specificity
D015342:DNA Probes; D012336:RNA, Ribosomal, 16S