Excimer Laser Assisted Non-occlusive Anastomosis (ELANA). Our experience with a training model in vivo.
OBJECTIVE
In this study the ELANA Technique has been reproduced in our experimental laboratory in order to verify its feasibility and reproducibility, the percentage of patent anastomosis in acute at different steps along the learning curve of the surgical team, specific problems related to the surgical technique.


METHODS
In 20 rabbits New Zealand 4kg body weight the training model in vivo proposed by Tulleken and coworkers has been reproduced, realizing 40 ELANA anastomosis. The model consists in the realization of two different end-to-side anastomosis on the abdominal aorta of each experimental animal, assisted by a special designed suction/excimer laser catheter, then connected by an end-to-end suture. After a few hours the animals are sacrificed and the by-pass site withdrawn and examined in order to verify the percentage of patency in acute.


RESULTS
In the first 5 animals (group A), the anastomosis were realized using a jugular vein graft and the procedure results successful in only 3 cases out of ten (30%). For the following experiments - groups B, C and D where an aorta artery graft was used, the percentage of arterial flap retrieval was respectively 50%, 60% and 80%.


CONCLUSIONS
ELANA is a feasible fascinating microsurgical technique for the realization of high flow, non-occlusive anastomosis. The rate of success results progressively higher along the learning curve of the surgical team. In our opinion, before the application of the ELANA technique on humans, a period of propaedeutic training in vivo on laboratory animals is essential for the dedicated team.
Russo
G
G
Unit of Neurosurgery, AORN A. Cardarelli, Naples, Italy. giuseppe.russo@ospesdale/cardarelli.it
Rotondo
M
M
Punzo
A
A
Di Napoli
D
D
D000818:Animals; D001012:Aorta, Abdominal / Q000033:anatomy & histology / Q000502:physiology / Q000601:surgery*; D002548:Cerebral Revascularization / Q000295:instrumentation / Q000379:methods*; D006083:Graft Occlusion, Vascular / Q000209:etiology / Q000503:physiopathology / Q000517:prevention & control; D007601:Jugular Veins / Q000601:surgery / Q000637:transplantation; D053685:Laser Therapy; D023421:Models, Animal; D011183:Postoperative Complications / Q000209:etiology / Q000503:physiopathology / Q000517:prevention & control*; D011817:Rabbits; D012039:Regional Blood Flow / Q000502:physiology / Q000528:radiation effects; D013524:Surgical Flaps / Q000592:standards / Q000639:trends; D013663:Teaching / Q000379:methods; D016378:Tissue Transplantation / Q000295:instrumentation / Q000379:methods; D016896:Treatment Outcome; D014619:Vacuum Curettage / Q000295:instrumentation / Q000379:methods; D014654:Vascular Patency / Q000502:physiology / Q000528:radiation effects*