title: Rapid discrimination between Candida albicans and Candida dubliniensis by using real-time polymerase chain reaction.
abstract: Several phenotypic methods have been used for the differentiation of Candida albicans and Candida dubliniensis, but molecular investigations are considered most reliable in their diagnostic value. Here, we suggest a rapid real-time polymerase chain reaction assay where the discrimination was achieved through melting point analysis with the help of the nonspecific fluorescent dye SybrGreen.
authors: Institute of Clinical Microbiology, Faculty of Medicine, University of Szeged, Szeged 6725, Hungary. fsoma99@hotmail.com
authors: Ferenc
authors: F
authors: Somogyvari
authors: Ilona
authors: I
authors: Doczi
authors: Julianna
authors: J
authors: Serly
authors: Suhail
authors: S
authors: Ahmad
authors: Elizabeth
authors: E
authors: Nagy
mesh_terms: D052160:Benzothiazoles; D002175:Candida / Q000145:classification* / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D003959:Diamines; D009930:Organic Chemicals / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D011804:Quinolines
chemical_list: D052160:Benzothiazoles; D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer; D003959:Diamines; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I