title: Simultaneous analysis of cyclophosphamide, doxorubicin and doxorubicinol by liquid chromatography coupled to tandem mass spectrometry.
abstract: A method for the simultaneous determination of cyclophosphamide (CP), doxorubicin (dox), and doxorubicinol (dol) was developed and validated to analyze 400 microL of plasma from patients receiving chemotherapeutic treatment with CP and dox. Final calibration ranges for the analytes were 0.440-60.0 microg/mL for cyclophosphamide, 7.20-984 ng/mL for dox and 3.04-104 ng/mL for dol. The samples were prepared using solid phase extraction and analyzed using a gradient separation over a Waters Symmetry C18, 2.1 by 30 mm (Milford, MA) column. Detection was achieved in positive mixed reaction monitoring mode on a triple quadrupole mass spectrometer.
authors: Department of Pharmacy Practice, University at Buffalo, 311 Hochstetter Hall, Buffalo, NY 14260, USA. rda@buffalo.edu
authors: Robin
authors: R
authors: DiFrancesco
authors: Jennifer J
authors: JJ
authors: Griggs
authors: Julie
authors: J
authors: Donnelly
authors: Robert
authors: R
authors: DiCenzo
mesh_terms: D000971:Antineoplastic Combined Chemotherapy Protocols / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003520:Cyclophosphamide / Q000097:blood* / Q000493:pharmacokinetics; D004317:Doxorubicin / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004317:Doxorubicin; D003520:Cyclophosphamide; C010013:adriamycinol