Zinc uptake regulator (zur) gene involved in zinc homeostasis and virulence of Xanthomonas oryzae pv. oryzae in rice.
Xanthomonas oryzae pv. oryzae causes bacterial leaf blight, one of the most widespread and destructive bacterial diseases in rice. In order to understand the gene of zinc uptake regulator (zur) involved in virulence of the pathogen in rice, we generated a mutant OSZRM by homologous suicide plasmid integration. The mutant failed to grow in NYGB medium supplemented with Zn(2+) or Fe(3+) at a concentration of 500 muM or 6 mM, whereas the wild-type strain grew well at the same conditions. The zur mutant was hypersensitive to hydrogen peroxide and exhibited reduction catalase activity and the production of extracellular polysaccharide (EPS). Interestingly, the mutant showed a reduction in virulence on rice but still kept triggering hypersensitive response (HR) in tobacco. When the mutant was complemented with the zur gene, the response was recovered to wild-type. These results suggested that zur gene is a functional member of the Zur regulator family that controls zinc and iron homeostasis, oxidative stress, and EPS production, which is necessary for virulence in X. oryzae pv. oryzae.
Yang
Wanfeng
W
Department of Plant Pathology, Nanjing Agricultural University, Nanjing, 210095, Republic of China.
Liu
Yan
Y
Chen
Lei
L
Gao
Tongchun
T
Hu
Baishi
B
Zhang
Dongfang
D
Liu
Fengquan
F
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D001692:Biological Transport / Q000187:drug effects / Q000235:genetics; D015139:Blotting, Southern; D002455:Cell Division / Q000187:drug effects / Q000235:genetics; D005290:Ferric Compounds / Q000494:pharmacology; D005816:Genetic Complementation Test; D006706:Homeostasis; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008969:Molecular Sequence Data; D009154:Mutation; D012275:Oryza / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014774:Virulence / Q000235:genetics; D014974:Xanthomonas / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D015032:Zinc / Q000378:metabolism / Q000493:pharmacokinetics* / Q000494:pharmacology
D001426:Bacterial Proteins; D005290:Ferric Compounds; D011135:Polysaccharides, Bacterial; D006861:Hydrogen Peroxide; D015032:Zinc