Protein oxidation implicated as the primary determinant of bacterial radioresistance.
In the hierarchy of cellular targets damaged by ionizing radiation (IR), classical models of radiation toxicity place DNA at the top. Yet, many prokaryotes are killed by doses of IR that cause little DNA damage. Here we have probed the nature of Mn-facilitated IR resistance in Deinococcus radiodurans, which together with other extremely IR-resistant bacteria have high intracellular Mn/Fe concentration ratios compared to IR-sensitive bacteria. For in vitro and in vivo irradiation, we demonstrate a mechanistic link between Mn(II) ions and protection of proteins from oxidative modifications that introduce carbonyl groups. Conditions that inhibited Mn accumulation or Mn redox cycling rendered D. radiodurans radiation sensitive and highly susceptible to protein oxidation. X-ray fluorescence microprobe analysis showed that Mn is globally distributed in D. radiodurans, but Fe is sequestered in a region between dividing cells. For a group of phylogenetically diverse IR-resistant and IR-sensitive wild-type bacteria, our findings support the idea that the degree of resistance is determined by the level of oxidative protein damage caused during irradiation. We present the case that protein, rather than DNA, is the principal target of the biological action of IR in sensitive bacteria, and extreme resistance in Mn-accumulating bacteria is based on protein protection.
Daly
Michael J
MJ
Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, Maryland, United States of America. mdaly@usuhs.mil
Gaidamakova
Elena K
EK
Matrosova
Vera Y
VY
Vasilenko
Alexander
A
Zhai
Min
M
Leapman
Richard D
RD
Lai
Barry
B
Ravel
Bruce
B
Li
Shu-Mei W
SM
Kemner
Kenneth M
KM
Fredrickson
James K
JK
D001426:Bacterial Proteins / Q000378:metabolism*; D034301:Deinococcus / Q000378:metabolism / Q000528:radiation effects*; D005453:Fluorescence; D007501:Iron / Q000378:metabolism; D008345:Manganese / Q000378:metabolism; D010084:Oxidation-Reduction; D011836:Radiation Tolerance
D001426:Bacterial Proteins; D008345:Manganese; D007501:Iron