title: [Cloning and characterization of genes differentially expressed in human dental pulp cells and gingival fibroblasts].
abstract: OBJECTIVE
  To study the biological properties of human dental pulp cells (HDPC) by cloning and analysis of genes differentially expressed in HDPC in comparison with human gingival fibroblasts (HGF).
  
  
  METHODS
  HDPC and HGF were cultured and identified by immunocytochemistry. HPDC and HGF subtractive cDNA library was established by PCR-based modified subtractive hybridization, genes differentially expressed by HPDC were cloned, sequenced and compared to find homogeneous sequence in GenBank by BLAST.
  
  
  RESULTS
  Cloning and sequencing analysis indicate 12 genes differentially expressed were obtained, in which two were unknown genes. Among the 10 known genes, 4 were related to signal transduction, 2 were related to trans-membrane transportation (both cell membrane and nuclear membrane), and 2 were related to RNA splicing mechanisms.
  
  
  CONCLUSIONS
  The biological properties of HPDC are determined by the differential expression of some genes and the growth and differentiation of HPDC are associated to the dynamic protein synthesis and secretion activities of the cell.
authors: Dept. of Endodontics, Guanghua College of Stomatology, Sun Yat-Sen University, Guangzhou 510055, China.
authors: Zhong-dong
authors: ZD
authors: Wang
authors: Ji-nan
authors: JN
authors: Wu
authors: Lin
authors: L
authors: Zhou
authors: Jun-qi
authors: JQ
authors: Ling
authors: Xi-min
authors: XM
authors: Guo
authors: Ming-zhen
authors: MZ
authors: Xiao
authors: Feng
authors: F
authors: Zhu
authors: Qin
authors: Q
authors: Pu
authors: Yu-bo
authors: YB
authors: Chai
authors: Zhong-liang
authors: ZL
authors: Zhao
mesh_terms: D003001:Cloning, Molecular; D019976:Cloning, Organism; D003782:Dental Pulp; D005347:Fibroblasts; D015723:Gene Library; D005881:Gingiva; D006801:Humans; D016133:Polymerase Chain Reaction