The old is new again: asparagine oxidation in calcium-dependent antibiotic biosynthesis.
Non-ribosomal peptides are built from both proteinogenic and non-proteinogenic amino acids. The latter resemble amino acids but contain modifications not found in proteins. The recent characterization of a non-heme Fe(2+) and alpha-ketoglutarate-dependent oxygenase that stereospecifically generates beta-hydroxyasparagine, an unnatural amino acid building block for the biosynthesis of calcium-dependent antibiotic, a lipopeptide antibiotic. This work improves our understanding of how these non-proteinogenic amino acids are synthesized.
Worthington
Andrew S
AS
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093-0358, USA.
Burkart
Michael D
MD
D000596:Amino Acids; D001216:Asparagine / Q000031:analogs & derivatives / Q000378:metabolism*; D053858:Metabolic Networks and Pathways; D010084:Oxidation-Reduction; D046908:Peptide Biosynthesis, Nucleic Acid-Independent; D010455:Peptides; D013302:Streptomyces / Q000378:metabolism; D048372:Streptomyces coelicolor / Q000737:chemistry*
D000596:Amino Acids; C040487:CDA antibiotic; D010455:Peptides; C050931:3-hydroxyasparagine; D001216:Asparagine