Multiplex PCR assay for rapid and accurate capsular typing of group B streptococci.
We developed a simple, specific, and sensitive two-multiplex-PCR assay that enabled the detection of all known group B streptococcal (GBS) capsular polysaccharides. This test is well adapted for GBS capsular polysaccharide typing in large-scale epidemiological studies.
Poyart
Claire
C
Service de Bactériologie Groupe Hospitalier Cochin-Saint Vincent de Paul, Assitance Publique-Hôpitaux de Paris, 27 rue du Faubourg Saint Jacques, 75014 Paris, France. claire.poyart@cch.aphp.fr
Tazi
Asmaa
A
Réglier-Poupet
Hélène
H
Billoët
Annick
A
Tavares
Nicole
N
Raymond
Josette
J
Trieu-Cuot
Patrick
P
D016667:Bacterial Capsules / Q000737:chemistry / Q000145:classification* / Q000235:genetics; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013290:Streptococcal Infections / Q000453:epidemiology* / Q000382:microbiology; D013292:Streptococcus agalactiae / Q000145:classification* / Q000235:genetics; D013997:Time Factors