title: Transduced PEP-1-Grb7 fusion protein suppressed LPS-induced COX-2 expression.
abstract: Although the incidence and severity of atopic dermatitis (AD) is steadily increasing at an alarming rate, its pathogenic mechanisms remain poorly understood yet. Recently, we found that the expression of Grb7 protein was markedly decreased in AD patients using proteomic analysis. In the present study, human Grb7 gene was fused with PEP-1 peptide in a bacterial expression vector to produce a genetic in-frame PEP-1-Grb7 fusion protein. The expressed and purified PEP-1-Grb7 fusion proteins transduced efficiently into skin cells in a time- and dose-dependent manner when added exogenously in culture media. Once inside the cells, the transduced PEP-1-Grb7 protein was stable for 48 h. In addition, transduced PEP-1-Grb7 fusion protein markedly increased cell viability in macrophage RAW 264.7 cells treated with LPS by inhibition of the COX-2 expression level. These results suggest that the PEP-1-Grb7 fusion protein can be used in protein therapy for inflammatory skin disorders, including AD.
authors: Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chunchon 200-702, Korea.
authors: Jae Jin
authors: JJ
authors: An
authors: So Young
authors: SY
authors: Kim
authors: Sun Hwa
authors: SH
authors: Lee
authors: Dae Won
authors: DW
authors: Kim
authors: Hea Jin
authors: HJ
authors: Ryu
authors: Seung Il
authors: SI
authors: Yeo
authors: Sang Ho
authors: SH
authors: Jang
authors: Hyung Joo
authors: HJ
authors: Kwon
authors: Tae Yoon
authors: TY
authors: Kim
authors: Sang Chul
authors: SC
authors: Lee
authors: Haryoung
authors: H
authors: Poo
authors: Sung-Woo
authors: SW
authors: Cho
authors: Kil Soo
authors: KS
authors: Lee
authors: Jinseu
authors: J
authors: Park
authors: Won Sik
authors: WS
authors: Eum
authors: Soo Young
authors: SY
authors: Choi
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D003543:Cysteamine / Q000031:analogs & derivatives* / Q000378:metabolism; D015536:Down-Regulation; D005347:Fibroblasts / Q000187:drug effects; D051382:GRB7 Adaptor Protein / Q000235:genetics / Q000378:metabolism*; D005822:Genetic Vectors; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D014161:Transduction, Genetic
chemical_list: D008070:Lipopolysaccharides; C503924:Pep-1 peptide; D010455:Peptides; D011993:Recombinant Fusion Proteins; D051382:GRB7 Adaptor Protein; D003543:Cysteamine; D051546:Cyclooxygenase 2