title: Polymorphic microRNA-target interactions: a novel source of phenotypic variation.
abstract: Studying the muscular hypertrophy of Texel sheep by forward genetics, we have identified an A-to-G transition in the 3'UTR of the GDF8 gene that reveals an illegitimate target site for microRNAs miR-1 and miR-206 that are highly expressed in skeletal muscle. This causes the down-regulation of this muscle-specific chalone and hence contributes to the muscular hypertrophy of Texel sheep. We demonstrate that polymorphisms which alter the content of putative miRNA target sites are common in human and mice, and provide evidence that both conserved and nonconserved target sites are selectively constrained. We speculate that these polymorphisms might be important mediators of phenotypic variation including disease. To facilitate studies along those lines, we have constructed a database (www.patrocles.org) listing putative polymorphic microRNA-target interactions.
authors: Unit of Animal Genomics, Department of Animal Production, Faculty of Veterinary Medicine & CBIG, University of Liège (B43), Liège, Belgium.
authors: M
authors: M
authors: Georges
authors: A
authors: A
authors: Clop
authors: F
authors: F
authors: Marcq
authors: H
authors: H
authors: Takeda
authors: D
authors: D
authors: Pirottin
authors: S
authors: S
authors: Hiard
authors: X
authors: X
authors: Tordoir
authors: F
authors: F
authors: Caiment
authors: F
authors: F
authors: Meish
authors: B
authors: B
authors: Bibé
authors: J
authors: J
authors: Bouix
authors: J M
authors: JM
authors: Elsen
authors: F
authors: F
authors: Eychenne
authors: E
authors: E
authors: Laville
authors: C
authors: C
authors: Larzul
authors: D
authors: D
authors: Milenkovic
authors: J
authors: J
authors: Tobin
authors: And C
authors: AC
authors: Charlier
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D002874:Chromosome Mapping; D030541:Databases, Genetic; D005260:Female; D006801:Humans; D006984:Hypertrophy / Q000235:genetics; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D024510:Muscle Development / Q000235:genetics; D009132:Muscles / Q000473:pathology; D010641:Phenotype; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D014176:Protein Biosynthesis; D040641:Quantitative Trait Loci; D012641:Selection, Genetic; D012756:Sheep / Q000235:genetics / Q000254:growth & development
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs