Optimized determination method for trans-10-hydroxy-2-decenoic acid content in royal jelly by high-performance liquid chromatography with an internal standard.
An optimized reversed-phase high-performance liquid chromatography method was developed to detect the trans-10-hydroxy-2-decenoic acid (10-HDA) content in royal jelly cream and lyophilized powder. The sample was extracted using absolute ethanol. Chromatographic separation of 10-HDA and methyl 4-hydroxybenzoate as the internal standard was performed on a Nova-pak C18 column. The average recoveries were 95.0-99.2% (n = 5) with relative standard deviation (RSD) values of 1.3-2.1% for royal jelly cream and 98.0-100.0% (n = 5) with RSD values of 1.6-3.0% for lyophilized powder, respectively. The limits of detection and quantitation were 0.5 and 1.5 mg/kg, respectively, for both royal jelly cream and lyophilized powder. The method was validated for the determination of practical royal jelly products. The concentration of 10-HDA ranged from 1.26 to 2.21% for pure royal jelly cream samples and 3.01 to 6.19% for royal jelly lyophilized powder samples. For 30 royal jelly products, the 10-HDA content varied from not detectable to 0.98%.
Zhou
Jinhui
J
Beeproduct Quality Supervision and Inspection Testing Center, Ministry of Agriculture, Beijing 100093, People's Republic of China.
Xue
Xiaofeng
X
Li
Yi
Y
Zhang
Jinzhen
J
Zhao
Jing
J
D000818:Animals; D001516:Bees; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005227:Fatty Acids / Q000737:chemistry*; D005229:Fatty Acids, Monounsaturated / Q000032:analysis*; D005612:Freeze Drying; D007202:Indicators and Reagents; D007700:Kinetics; D000099219:Royal Jelly
D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D007202:Indicators and Reagents; D000099219:Royal Jelly; C055543:10-hydroxy-2-decenoic acid