Development of an HPLC method for the determination of doripenem in human and mouse serum.
A HPLC method utilizing solid phase extraction was developed to analyze doripenem (formerly S-4661) in human and mouse serum. A reversed-phase column was used with a UV detector set at 295 nm. The mobile phase consisted of methanol and phosphate buffer at a flow rate of 1.5 ml/min. Meropenem was used as the internal standard. The standard curve was linear over a range of 0.5-40 microg/ml. The assay is simple, reproducible, and accurate and has been used successfully to analyze doripenem concentrations from a murine pharmacokinetic study.
Sutherland
Christina
C
Center for Anti-Infective Research and Development, Hartford Hospital, Hartford, CT 06102, United States.
Nicolau
David P
DP
D000818:Animals; D015780:Carbapenems / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000077726:Doripenem; D006801:Humans; D051379:Mice; D015203:Reproducibility of Results
D015780:Carbapenems; D000077726:Doripenem