title: Prospective study on dietary intakes of folate, betaine, and choline and cardiovascular disease risk in women.
abstract: OBJECTIVE
  To investigate the association between dietary intakes of folate, betaine and choline and the risk of cardiovascular disease (CVD).
  
  
  METHODS
  Prospective cohort study.
  
  
  METHODS
  A total of 16 165 women aged 49-70 years without prior CVD. SUBJECTS were breast cancer screening participants in the PROSPECT-EPIC cohort, which is 1 of the 2 Dutch contributions to the European Prospective Investigation into Cancer and Nutrition (EPIC).
  
  
  METHODS
  Each participant completed a validated food frequency questionnaire. Folate intake was calculated with the Dutch National Food Database. Betaine and choline intakes were calculated with the USDA database containing choline and betaine contents of common US foods. Data on coronary heart disease (CHD) events and cerebrovascular accident (CVA) events morbidity data were obtained from the Dutch Centre for Health Care Information.
  
  
  RESULTS
  During a median follow-up period of 97 months, 717 women were diagnosed with CVD. After adjustment, neither folate, nor betaine, nor choline intakes were associated with CVD (hazard ratios for highest versus lowest quartile were 1.23 (95% confidence interval 0.75; 2.01), 0.90 (0.69; 1.17), 1.04 (0.71; 1.53), respectively). In a subsample of the population, high folate and choline intakes were statistically significantly associated with lower homocysteine levels. High betaine intake was associated with slightly lower high-density lipoprotein (HDL)-cholesterol concentrations.
  
  
  CONCLUSIONS
  Regular dietary intakes of folate, betaine and choline were not associated with CVD risk in post-menopausal Dutch women. However, the effect of doses of betaine and choline beyond regular dietary intake--for example, via supplementation or fortification--remains unknown.
authors: Wageningen University, Wageningen, The Netherlands.
authors: G W
authors: GW
authors: Dalmeijer
authors: M R
authors: MR
authors: Olthof
authors: P
authors: P
authors: Verhoef
authors: M L
authors: ML
authors: Bots
authors: Y T
authors: YT
authors: van der Schouw
mesh_terms: D000368:Aged; D001622:Betaine / Q000008:administration & dosage* / Q000097:blood; D002318:Cardiovascular Diseases / Q000097:blood / Q000453:epidemiology*; D002794:Choline / Q000008:administration & dosage* / Q000097:blood; D015331:Cohort Studies; D004032:Diet; D005260:Female; D005492:Folic Acid / Q000008:administration & dosage* / Q000097:blood; D005500:Follow-Up Studies; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D008875:Middle Aged; D009426:Netherlands / Q000453:epidemiology; D009749:Nutrition Surveys; D016017:Odds Ratio; D017698:Postmenopause; D011446:Prospective Studies; D011795:Surveys and Questionnaires
chemical_list: D006710:Homocysteine; D001622:Betaine; D005492:Folic Acid; D002794:Choline