[Detection of Mycobacterium tuberculosis DNA in sarcoidosis samples using real-time fluorescence polymerase chain reaction].
OBJECTIVE
To study the role of Mycobacterium tuberculosis in the pathogenesis of sarcoidosis.


METHODS
Archival material of 22 patients with a histologic diagnosis of sarcoidosis were retrieved. Real-time fluorescent polymerase chain reaction (PCR) was used to detect DNA fragments of the complex-specific insertion sequence IS6110 of Mycobacterium tuberculosis in formalin-fixed and paraffin-embedded biopsy samples.


RESULTS
Among the 22 samples studied, Mycobacterium tuberculosis DNA was detected in 11 cases. The sequence of PCR amplified IS6110 DNA fragments completely matched with the related sequence in Mycobacterium tuberculosis gene.


CONCLUSIONS
Mycobacterium tuberculosis DNA is identified in a certain proportion of patients with a clinicopathologic diagnosis of sarcoidosis. Mycobacterium tuberculosis may be an important etiologic agent, at least in some of these patients.
Xu
Mei-lin
ML
Department of Pathology, Tianjin Chest Hospital, Tianjin 300051, China.
Zhong
Hao-hao
HH
Heng
Wan-jie
WJ
Wu
Bing-quan
BQ
D000328:Adult; D004269:DNA, Bacterial / Q000032:analysis*; D005260:Female; D005453:Fluorescence; D005500:Follow-Up Studies; D006801:Humans; D008198:Lymph Nodes / Q000382:microbiology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction / Q000379:methods*; D012507:Sarcoidosis / Q000382:microbiology* / Q000473:pathology
D004269:DNA, Bacterial