[Relationship between genotypes at MyoD locus and carcass traits in cattle].
A 261 bp sequence of the bovine MyoD gene intron 2 was cloned and was found to bear no similarities to the human MyoD gene sequence. Polymorphisms of the Myod gene in cattle including three Chinese breeds (Luxi cattle, Jinnan cattle and Qinchuan cattle) and four cross-breeding populations (Limousin x Luxi cattle, Simmental x Luxi cattle, Charolais x Luxi cattle and Angus x Luxi cattle) were detected by PCR-SSCP. Two SSCP alleles (A and B) were detected, which caused by a C-->T at 39 bp and a C-->G transition at 112 bp. Only two genotypes AA and AB occurred in the population. In Chinese local cattle, B allele was dominant, and this locus was at Hardy-Weinberg equilibrium except for the Charolais x Luxi cattle and Angus x Luxi cattle. The association of these polymorphisms with cattle carcass traits was analyzed using the general linear model (GLM). Statistical analysis revealed a higher value of living weight, carcass weight and loin eye area for individuals with genotype AA than AB (P < 0.05). Further studies on a bigger population size are needed to confirm the observed effect of MyoD genotypes on carcass quality traits.
Tian
Lu
L
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China. bvcajessica2005@163.com
Xu
Shang-Zhong
SZ
Yue
Wen-Bin
WB
Li
Jun-Ya
JY
Gao
Xue
X
Ren
Hong-Yan
HY
D000483:Alleles; D000818:Animals; D002417:Cattle; D005260:Female; D005787:Gene Frequency; D008040:Genetic Linkage; D005838:Genotype; D008297:Male; D017570:MyoD Protein / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational
D017570:MyoD Protein