Isolation of human oral keratinocyte progenitor/stem cells.
Progenitor/stem cell populations of epithelium are known to reside in the small-sized cell population. Our objective was to physically isolate and characterize an oral keratinocyte-enriched population of small-sized progenitor/stem cells. Primary human oral mucosal keratinocytes cultured in a chemically defined serum-free culture system, devoid of animal-derived feeder cells, were sorted by relative cell size and characterized by immunolabeling for beta1 integrin, nuclear transcription factor, peroxisome proliferator-activated receptor-gamma, and cell-cycle analysis. Sorted cells were distinguished as progenitor/stem cells by functional assays and their ability to regenerate an oral mucosal graft. Small-sized cells demonstrated the lowest expression of peroxisome proliferator-activated receptor-gamma, the highest colony-forming efficiency, a longer long-term proliferative potential, an enriched quiescent cell population, and the ability to regenerate an oral mucosal graft, implying that the small-sized cultured oral keratinocytes contained an enriched population of progenitor/stem cells.
Izumi
K
K
Department of Oral and Maxillofacial Surgery, University of Michigan Health System, B1-208 TC, Box 0018, 1500 East Medical Center Drive, Ann Arbor, MI 48109-0018, USA.
Tobita
T
T
Feinberg
S E
SE
D000293:Adolescent; D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods*; D048429:Cell Size; D002648:Child; D003114:Colony-Forming Units Assay; D005260:Female; D006801:Humans; D019012:Integrin beta1 / Q000032:analysis / Q000096:biosynthesis; D015603:Keratinocytes / Q000166:cytology*; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D047495:PPAR gamma / Q000032:analysis / Q000096:biosynthesis; D012038:Regeneration; D013234:Stem Cells; D046509:Tissue Culture Techniques; D023822:Tissue Engineering / Q000379:methods*
D019012:Integrin beta1; D047495:PPAR gamma