title: Purification, structure and immunobiological activity of a new water-soluble polysaccharide from the mycelium of Polyporus albicans (Imaz.) Teng.
abstract: A new water-soluble intracellular polysaccharide named as PTP, with a molecular mass of 3.7x10(4) Da, was obtained from the mycelium of Polyporus albicans (Imaz.) Teng. Structure features of the purified polysaccharide were investigated by a combination of chemical and instrumental analysis. The results indicated that PTP consisted of a backbone composed of (1-->3)-linked-beta-d-mannopyranosyl, (1-->3,6)-linked-beta-d-mannopyranosyl and (1-->6)-linked-alpha-d-galactopyranosyl residues in the ratio of 3:1:1, and terminated with a single non-reducing terminal (1-->)-beta-d-mannopyranosyl residues at the C-6 position of (1-->3,6)-linked-beta-d-mannopyranosyl, along the main chain. This is the first report describing the isolation and structure elucidation of a new intracellular polysaccharide produced from the mycelium of P. albicans (Imaz.) Teng. Preliminary tests in vitro showed PTP have potent stimulating effects on murine lymphocyte proliferation induced by concanavalin A or lipopolysaccharide and its branches are extremely important for the expression of the enhancement of the immunological activity.
authors: College of Life Sciences, Northeast Normal University, Changchun 130024, China.
authors: Yongxu
authors: Y
authors: Sun
authors: Shusheng
authors: S
authors: Wang
authors: Tianbao
authors: T
authors: Li
authors: Xia
authors: X
authors: Li
authors: Lili
authors: L
authors: Jiao
authors: Liping
authors: L
authors: Zhang
mesh_terms: D000818:Animals; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005658:Fungi / Q000378:metabolism*; D008297:Male; D051379:Mice; D025282:Mycelium / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry* / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D012995:Solubility; D014867:Water / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; D014867:Water