title: The structure-function relationship of WspR, a Pseudomonas fluorescens response regulator with a GGDEF output domain.
abstract: The GGDEF response regulator WspR couples the chemosensory Wsp pathway to the overproduction of acetylated cellulose and cell attachment in the Pseudomonas fluorescens SBW25 wrinkly spreader (WS) genotype. Here, it is shown that WspR is a diguanylate cyclase (DGC), and that DGC activity is elevated in the WS genotype compared to that in the ancestral smooth (SM) genotype. A structure-function analysis of 120 wspR mutant alleles was employed to gain insight into the regulation and activity of WspR. Firstly, 44 random and defined pentapeptide insertions were produced in WspR, and the effects determined using assays based on colony morphology, attachment to surfaces and cellulose production. The effects of mutations within WspR were interpreted using a homology model, based on the crystal structure of Caulobacter crescentus PleD. Mutational analyses indicated that WspR activation occurs as a result of disruption of the interdomain interface, leading to the release of effector-domain repression by the N-terminal receiver domain. Quantification of attachment and cellulose production raised significant questions concerning the mechanisms of WspR function. The conserved RYGGEEF motif of WspR was also subjected to mutational analysis, and 76 single amino acid residue substitutions were tested for their effects on WspR function. The RYGGEEF motif of WspR is functionally conserved, with almost every mutation abolishing function.
authors: Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK.|Division of Molecular Microbiology, Biozentrum, Klingelbergstrasse 50-70, CH-4056 Basel, Switzerland.
authors: J G
authors: JG
authors: Malone
authors: Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK.
authors: R
authors: R
authors: Williams
authors: Division of Molecular Microbiology, Biozentrum, Klingelbergstrasse 50-70, CH-4056 Basel, Switzerland.
authors: M
authors: M
authors: Christen
authors: Division of Molecular Microbiology, Biozentrum, Klingelbergstrasse 50-70, CH-4056 Basel, Switzerland.
authors: U
authors: U
authors: Jenal
authors: Centre for Ecology and Hydrology, Mansfield Road, Oxford OX1 3SR, UK.|Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK.
authors: A J
authors: AJ
authors: Spiers
authors: School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand.|Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK.
authors: P B
authors: PB
authors: Rainey
mesh_terms: D020816:Amino Acid Motifs; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins; D008958:Models, Molecular; D010641:Phenotype; D019761:Phosphorus-Oxygen Lyases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D011551:Pseudomonas fluorescens / Q000201:enzymology* / Q000235:genetics; D013329:Structure-Activity Relationship
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D019761:Phosphorus-Oxygen Lyases; C060720:diguanylate cyclase