title: Development of new-generation HU-PBMC-NOD/SCID mice to study human islet alloreactivity.
abstract: The use of "humanized" mice represents an appealing translational model for studies of the pathogenesis of immune-mediated diseases and for the evaluation of potential therapeutics. The utility of humanized mice depends on their ability to model the human immune system with high fidelity, and, in this respect, previous models have fallen short. The recently developed NOD-scid Il2rgamma(null) mouse, however, exhibits greatly enhanced ability to support the engraftment of human peripheral blood mononuclear cells. Herein, we describe the challenges of recapitulating human immunity in humanized mice and features of NOD-scid Il2rgamma(null) mice that help overcome them.
authors: Diabetes Division, University of Massachusetts Medical School, 373 Plantation Street, Suite 218, Worcester, MA 01605, USA.
authors: Marie
authors: M
authors: King
authors: Todd
authors: T
authors: Pearson
authors: Leonard D
authors: LD
authors: Shultz
authors: Jean
authors: J
authors: Leif
authors: Rita
authors: R
authors: Bottino
authors: Massimo
authors: M
authors: Trucco
authors: Mark
authors: M
authors: Atkinson
authors: Clive
authors: C
authors: Wasserfall
authors: Kevan
authors: K
authors: Herold
authors: John P
authors: JP
authors: Mordes
authors: Aldo A
authors: AA
authors: Rossini
authors: Dale L
authors: DL
authors: Greiner
mesh_terms: D000818:Animals; D003433:Crosses, Genetic; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D004195:Disease Models, Animal; D006801:Humans; D007515:Islets of Langerhans / Q000276:immunology*; D007519:Isoantigens / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016688:Mice, Inbred NOD / Q000235:genetics*; D016513:Mice, SCID / Q000235:genetics*
chemical_list: D007519:Isoantigens