title: Structure and cell composition of spheres cultured from human fetal retina.
abstract: The structure and cell composition of spheres obtained by culturing human fetal retinal cells after 15, 18, 22-23, and 24 weeks of gestation were studied. The cells were cultured as neurospheres: in serum-free medium with growth factors, in which they formed floating spheres. Immunocytochemical analysis showed that cell proliferation in the spheres decreased with increasing fetal age. Stem/progenitor cells, neuroblasts, and photoreceptors were detected in the spheres. Glial cells were detected only in spheres originating from 22- and 24-week fetuses. All spheres, irrespective of age and duration of culturing, consisted of numerous cell rosettes, each histotypically similar to the neuroblastic layer of the developing retina.
authors: N. K. Koltsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow. aleksandrova@vigg.ru
authors: M A
authors: MA
authors: Aleksandrova
authors: O V
authors: OV
authors: Podgornyi
authors: R A
authors: RA
authors: Poltavtseva
authors: I G
authors: IG
authors: Panova
authors: G T
authors: GT
authors: Sukhikh
mesh_terms: D002449:Cell Aggregation / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D005333:Fetus; D005865:Gestational Age; D006801:Humans; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence; D012160:Retina / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology*