title: Effect of interferon-gamma treatment on the expression of interleukin-1beta at the maternal-fetal interface of pregnant rats.
abstract: In the present study, the possible mechanisms by which interferon (IFN)-gamma affects pregnancy were investigated using the cytokine network model. The IFN-gamma-induced expression of interleukin (IL)-1beta was examined using western blotting, immunohistochemistry and immunofluorescence. The results showed that IFN-gamma treatment significantly decreased the expression of uterine IL-1beta protein during the preimplantation, post-implantation and mid-gestation periods. The expression of IL-1beta protein was increased after IFN-gamma treatment compared with the control group in late pregnancy. In the placenta, IL-1beta protein levels were significantly increased after IFN-gamma treatment in early and mid-pregnancy. In late pregnancy, IFN-gamma treatment significantly decreased placental IL-1beta protein levels. IL-1beta was mainly expressed in the myometrium, uterine arteries, decidua basalis, trophospongium of the junctional layer and trophoblastic epithelium of the labyrinthine layers. IL-1beta was mainly located in the cytoplasm of in vitro cultured endometrial stromal cells (ESCs). IFN-gamma treatment did not affect the distribution of IL-1beta, only the expression of IL-1beta. The effects of IFN-gamma on the proliferation of ESCs were determined using an MTS (a novel tetrazolium compound) assay. IFN-gamma treatment inhibited the proliferation of ESCs and decreased the weight of the fetus and placenta. These results indicate that exogenous IFN-gamma affects the expression of IL-1beta and inhibits ESC proliferation.
authors: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy Sciences, Beijing 100080, China.
authors: Hong-Fei
authors: HF
authors: Xia
authors: Quan-Hong
authors: QH
authors: Sun
authors: Jing-Pian
authors: JP
authors: Peng
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D004717:Endometrium / Q000166:cytology / Q000187:drug effects; D005260:Female; D005333:Fetus / Q000187:drug effects* / Q000276:immunology; D007371:Interferon-gamma / Q000494:pharmacology*; D053583:Interleukin-1beta / Q000032:analysis / Q000378:metabolism*; D010920:Placenta / Q000737:chemistry / Q000187:drug effects* / Q000276:immunology; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D017154:Stromal Cells / Q000187:drug effects; D014599:Uterus / Q000187:drug effects / Q000276:immunology
chemical_list: D053583:Interleukin-1beta; D007371:Interferon-gamma