Primary central nervous system lymphoma secretes monocyte chemoattractant protein 1.
The majority of primary central nervous system lymphomas (PCNSL) are diffuse large B-cell lymphomas. Histologically, reactive T lymphocytes and mono-histiocytic cells are found within PCNSL tissue. To clarify the mechanisms of the cellular infiltration, the presence of monocyte chemoattractant protein (MCP-1) was investigated in biopsy samples of 19 cases of PCNSL by means of immunohistochemical staining, double staining with a confocal laser microscope, and Western blot analysis. MCP-1 expression was observed in all PCNSL immunohistochemically. Western blot analysis showed that the concentration of MCP-1 in PCNSL was as high as that in a metastatic brain tumor. In normal brain tissue, MCP-1 was not detected. Confocal laser microscope revealed MCP-1 signals were present in the cells with CD20, a B-cell marker. We concluded that lymphoma cells produced MCP-1, which is an additional cytokine involved in the pathogenesis of PCNSL.
Kitai
Ryuhei
R
Department of Neurosurgery, Faculty of Medical Sciences, University of Fukui, 23 Shimoaizuki, Eiheiji, Yoshida, Fukui 910-1193, Japan. rkitai@hotmail.com
Ishisaka
Kazuyo
K
Sato
Kazufumi
K
Sakuma
Takahiro
T
Yamauchi
Takahiro
T
Imamura
Yoshiaki
Y
Matsumoto
Hideki
H
Kubota
Toshihiko
T
D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000378:metabolism; D018951:Antigens, CD20 / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology*; D018932:Chemokine CCL2 / Q000378:metabolism*; D005260:Female; D006801:Humans; D016393:Lymphoma, B-Cell / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D000097382:CD68 Molecule
D015703:Antigens, CD; D018951:Antigens, CD20; D015214:Antigens, Differentiation, Myelomonocytic; D018932:Chemokine CCL2; C111835:CCL2 protein, human; C067980:CD68 antigen, human; D000097382:CD68 Molecule