DNA-directed immobilization of horseradish peroxidase-DNA conjugates on microelectrode arrays: towards electrochemical screening of enzyme libraries.
This work is aimed towards the generation of enzyme arrays on electrochemically active surfaces by taking advantage of the DNA-directed immobilization (DDI) technique. To this end, two different types of horseradish peroxidase (HRP)-DNA conjugates were prepared, either by covalent coupling with a bifunctional cross-linker or by the reconstitution of apo-HRP, that is, HRP lacking its prosthetic heme (protoporphyrin IX) group, with a covalently DNA-modified heme cofactor. Both conjugates were characterized in bulk and also subsequent to their immobilization on gold electrodes through specific DNA hybridization. Electrochemical measurements by using the phenolic mediator ortho-phenylendiamine indicated that, due to the high degree of conformational orientation, the apparent Michaelis-Menten constants of the reconstituted HRP conjugate were lower than those of the covalent conjugate. Due to the reversible nature of DDI, both conjugates could be readily removed from the electrode surface by simple washing and, subsequently, the electrodes could be reloaded with fresh enzymes, thereby restoring the initial amperometric-response activity. Moreover, the specific DNA hybridization allowed us to direct the two conjugates to distinct sites on a microelectrode array. Therefore, the self-assembly and regeneration capabilities of this approach should open the door to the generation of arrays of redox-enzyme devices for the screening of enzymes and their effectors.
Fruk
Ljiljana
L
Universität Dortmund, Fachbereich Chemie, Biologisch-Chemische Mikrostrukturtechnik, Otto-Hahn Strasse 6, 44227 Dortmund, Germany.
Müller
Joachim
J
Weber
Günther
G
Narváez
Arántzazu
A
Domínguez
Elena
E
Niemeyer
Christof M
CM
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002853:Chromatography, Liquid; D004796:Clinical Enzyme Tests; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes; D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006046:Gold / Q000737:chemistry; D006418:Heme / Q000737:chemistry / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D008839:Microelectrodes; D010084:Oxidation-Reduction; D010636:Phenols / Q000737:chemistry; D010655:Phenylenediamines / Q000737:chemistry; D011524:Protoporphyrins / Q000737:chemistry / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet
D015342:DNA Probes; D004800:Enzymes, Immobilized; D010636:Phenols; D010655:Phenylenediamines; D011524:Protoporphyrins; D006418:Heme; D006046:Gold; C034193:1,2-diaminobenzene; D004247:DNA; C028025:protoporphyrin IX; D006735:Horseradish Peroxidase