[Experimental study of the permeability of Fel Ursi's in eye].
OBJECTIVE
To determine whether Tauroursodeoxycholic acid (TUDCA) can penetrate the blood-ocular barrier after orally administration of Fel Ursi.


METHODS
56 rabbits were divided into two groups, 48 rabbits were used in experimental group, and other 8 rabbits were served as control. 100 mg/ml Fel Ursi were a fused into rabbits stomach. 2 ml blood from vein of auris-edge, aqueous humor from left eye and vitreous sample from right eye were obtained at 0.5, 1.0, 2.0, 4.0, 6.0 and 8.0 h after Fel Ursi administration. Concentration of TUDCA from all of samples was determined by HPLC.


RESULTS
TUDCA Concentrations were (999.1 +/- 17.2) - (1300.6 +/- 78.2) microg/ml, (12.7 +/- 1.4) - (47.8 +/- 4.7) microg/ml, and (10.8 +/- 2.9) - (57.9 +/- 7.9) microg/ml in blood, aqueous humor and vitreous respectively. There was no significant differences in the concentration of TUDCA in samples of aqueous humor and vitreous (P > 0.05). However the concentration of TUDCA in rabbit blood was much higher compared with that in aqueous humor and vitreous (P < 0.01).


CONCLUSIONS
Fel Ursi can reach intraocular tissue through penetrating blood-aqueous barrier and blood-vitreous barrier after orally application.
Cui
Hao
H
Eye Hospital, First Clinical College of Harbin Medical University, Harbin 150001, China.
Liu
Jing-jing
JJ
Fu
Shao-ying
SY
Liu
Hong-ling
HL
Hou
Yong-sheng
YS
Jin
Di
D
D000818:Animals; D018916:Blood-Aqueous Barrier / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005123:Eye / Q000187:drug effects; D010539:Permeability; D011817:Rabbits; D013655:Taurochenodeoxycholic Acid / Q000494:pharmacology*
D004365:Drugs, Chinese Herbal; D013655:Taurochenodeoxycholic Acid; C031655:ursodoxicoltaurine