Characterization of isoquinoline alkaloids from Neolitsea sericea var. aurata by HPLC-SPE-NMR.
Application of the HPLC-SPE-NMR technique to identify the alkaloids in an EtOH extract of the leaves of Neolitsea sericea var. aurata led to the characterization of 14 alkaloids while consuming plant material equivalent to 1.1 g. Of these, seven are N-oxides, four of which are new, namely, 9S,17S-pallidine Nalpha-oxide (6), 1S,2S-reticuline Nalpha-oxide (8), 6R,6aS-boldine Nbeta-oxide (9), and 6S,6aS-N-methyllaurotetanine Nalpha-oxide (13). Their structures were also confirmed by partial synthesis.
Lee
Shoei-Sheng
SS
School of Pharmacy, College of Medicine, National Taiwan University, Taipei 100, Taiwan, Republic of China, and Bruker Biospin GmbH, Silberstreifen, D-76287 Rheinstetten, Germany. shoeilee@ha.mc.ntu.edu.tw
Lai
Yi-Chun
YC
Chen
Chien-Kuang
CK
Tseng
Li-Hong
LH
Wang
Chen-Yu
CY
D000470:Alkaloids / Q000737:chemistry* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D007546:Isoquinolines / Q000737:chemistry* / Q000302:isolation & purification*; D019770:Lauraceae / Q000737:chemistry*; D008968:Molecular Conformation; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D018515:Plant Leaves / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013624:Taiwan
D000470:Alkaloids; D007546:Isoquinolines