Determination of patulin in apple juice: comparative evaluation of four analytical methods.
The performance of 4 purification methods for the analysis of patulin in apple juice was evaluated by high-performance liquid chromatography (HPLC). Samples were spiked with patulin at 10, 20, 50, 100, and 150 ppb (ng/mL) and extracted by one of 4 methods (3 solid-phase extraction and one liquid-liquid extraction), and then analyzed by HPLC-UV under the same isocratic conditions. The methods were validated for recovery, linearity, and precision at high and low concentrations. Recoveries were all >70% for spiking range 10-150 ppb. The relative standard deviation for repeatability was found to meet European Union Directive requirements. In addition, all the methods showed baseline separation from hydroxymethylfurfural.
Katerere
David R
DR
Programme on Mycotoxins and Experimental Carcinogenesis (PROMEC), Medical Research Council, PO Box 19070, Tygerberg 7505, South Africa. david.katerere@mrc.ac.za
Stockenström
Sonja
S
Balducci
Gabriele
G
Shephard
Gordon S
GS
D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005062:European Union; D005662:Furaldehyde / Q000031:analogs & derivatives / Q000302:isolation & purification; D027845:Malus / Q000737:chemistry*; D010365:Patulin / Q000737:chemistry / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014466:Ultraviolet Rays
C008046:5-hydroxymethylfurfural; D010365:Patulin; D005662:Furaldehyde