DNA-protein cross-link formation in Burkitt lymphoma cells cultured with benzaldehyde and the sedative paraldehyde.
Exposure to aldehydes represents potential risks to human and animal health. Cyclic aldehydes such as benzaldehyde, 2-furaldehyde, and paraldehyde were found to induce formation of stable DNA-protein cross-links (DPXs) in cultured human lymphoma cells. A relationship between increased cytotoxicity and DPX formation was observed with each aldehyde. Paraldehyde is a sedative drug used predominately in treatment of ethanol withdrawal. Paraldehyde was the most potent cross-linking aldehyde studied, yet least cytotoxic. Although DPX formation by aliphatic aldehydes is well-known, this study confirms the potential for cyclic aldehydes to cause formation of DPXs in cultured cells at therapeutically relevant doses.
Kuykendall
J R
JR
ChemRisk, Boulder, Colorado 80301, USA. jkuykendall@chemrisk.com
Jarvi
E J
EJ
Finley
B L
BL
Paustenbach
D J
DJ
D001547:Benzaldehydes / Q000494:pharmacology*; D002051:Burkitt Lymphoma / Q000378:metabolism*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism*; D005557:Formaldehyde / Q000494:pharmacology; D005662:Furaldehyde / Q000494:pharmacology; D006801:Humans; D010242:Paraldehyde / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism*
D001547:Benzaldehydes; D003432:Cross-Linking Reagents; D011506:Proteins; D005557:Formaldehyde; D004247:DNA; D005662:Furaldehyde; D010242:Paraldehyde; C032175:benzaldehyde