title: Monocyte chemoattractant protein-1 in obesity and type 2 diabetes. Insulin sensitivity study.
abstract: OBJECTIVE
  Our goal was to test any association between human plasma circulating levels of monocyte chemoattractant protein-1 (cMCP-1) and insulin resistance and to compare monocyte chemoattractant protein-1 (MCP-1) adipose tissue gene expression and cMCP-1 in relation with inflammatory markers.
  
  
  METHODS
  cMCP-1 was measured in n = 116 consecutive control male subjects to whom an insulin sensitivity (S(i)) test was performed. Circulating levels of soluble CD14, soluble tumor necrosis factor receptor type 2 (sTNFR2), soluble interleukin-6 (sIL-6), and adiponectin also were measured. Subcutaneous adipose tissue samples were obtained from n = 107 non-diabetic and type 2 diabetic subjects with different degrees of obesity. Real-time polymerase chain reaction was used to measure gene expression of MCP-1, CD68, tumor necrosis factor-alpha (TNF-alpha), and its receptor TNFR2.
  
  
  RESULTS
  In the S(i) study, no independent effect of cMCP-1 levels on insulin sensitivity was observed. In the expression study, in non-diabetic subjects, MCP-1 mRNA had a positive correlation with BMI (r = 0.407, p = 0.003), TNF-alpha mRNA (r = 0.419, p = 0.002), and TNFR2 mRNA (r = 0.410, p = 0.003). In these subjects, cMCP-1 was found to correlate with waist-to-hip ratio (r = 0.322, p = 0.048). In patients with type 2 diabetes, MCP-1 mRNA was up-regulated compared with non-diabetic subjects. TNF-alpha mRNA was found to independently contribute to MCP-1 mRNA expression. In this group, CD68 mRNA was found to correlate with BMI (r = 0.455, p = 0.001).
  
  
  CONCLUSIONS
  cMCP-1 is not associated with insulin sensitivity in apparently healthy men. TNF-alpha is the inflammatory cytokine associated with MCP-1 expression in subcutaneous adipose tissue.
authors: Research Department, University Hospital of Tarragona Joan XXIII, Rovira i Virgili University, Pere Virgili Institute, Tarragona, Spain.
authors: Matilde R
authors: MR
authors: Chacón
authors: José Manuel
authors: JM
authors: Fernández-Real
authors: Cristóbal
authors: C
authors: Richart
authors: Ana
authors: A
authors: Megía
authors: José Manuel
authors: JM
authors: Gómez
authors: Merce
authors: M
authors: Miranda
authors: Enric
authors: E
authors: Caubet
authors: Rosa
authors: R
authors: Pastor
authors: Carles
authors: C
authors: Masdevall
authors: Núria
authors: N
authors: Vilarrasa
authors: Wifredo
authors: W
authors: Ricard
authors: Joan
authors: J
authors: Vendrell
mesh_terms: D000328:Adult; D015992:Body Mass Index; D016022:Case-Control Studies; D018932:Chemokine CCL2 / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007333:Insulin Resistance / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D050151:Subcutaneous Fat / Q000378:metabolism
chemical_list: C111835:CCL2 protein, human; D018932:Chemokine CCL2; D012333:RNA, Messenger