[Study on the molecular mechanism of endostatin and doxycycline in suppressing melanoma growth].
OBJECTIVE
To investigate the molecular mechanism of endostatin and doxycycline effect on melanoma growth.


METHODS
A B16 melanoma mice model was established by intracutaneous injection of B16 cell suspension. The mice were treated with endostatin, doxycycline, endostatin and doxycycline respectively, the control group received no treatment. A time course study of tumor volume was performed to observe the antitumor effect. The expression of matrix metalloproteinase (MMP-9), MMP-2, TIMP-2 were examined by immunohistochemistry staining.


RESULTS
Tumors in endostatin treatment group, doxycycline treatment group, endostatin and doxycycline treatment group grew slower than in the control group. The difference of the average tumor volume in the doxycycline group and control group, in the doxycycline with endostatin treatment group and control group were statistically different. The positive expression ratio of MMP-2, MMP-9, TIMP-2 in each treatment group were statistically different from their control groups (F = 12.79, F = 5.56, F = 4.64; P < 0.05).


CONCLUSIONS
Doxycycline and endostatin are able to inhibit the expression of MMPs and promote expression of TIMP, which ultimately inhibits the growth of B16 melonoma.
Sun
Bao-cun
BC
Department of Pathology, Tianjin Medical University Cancer Hospital, Tianjin 300070, China. baocunsun@eyou.com
Zhang
Shi-wu
SW
Qi
Li-sha
LS
Zhang
Dan-fang
DF
Guo
Hua
H
Zhao
Xiu-lan
XL
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D045744:Cell Line, Tumor; D004318:Doxycycline / Q000494:pharmacology*; D004357:Drug Synergism; D043169:Endostatins / Q000494:pharmacology*; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000378:metabolism; D008546:Melanoma, Experimental / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000378:metabolism; D047368:Tumor Burden / Q000187:drug effects*
D000970:Antineoplastic Agents; D043169:Endostatins; D019716:Tissue Inhibitor of Metalloproteinase-2; D020782:Matrix Metalloproteinases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D004318:Doxycycline