title: Release of thioredoxin from Saccharomyces cerevisiae with environmental stimuli: solubilization of thioredoxin with ethanol.
abstract: Thioredoxin is crucial for the maintenance of the redox status of cells of all types. Mammalian thioredoxin is secreted from various types of cells, although the mechanism underlying has not yet been clarified. Previously, we demonstrated that thioredoxin was released from Saccharomyces cerevisiae after treatment with ethanol. In this paper, we show that as well as ethanol, low-pH shock and hypoosmotic shock release thioredoxin. Low-molecular-weight proteins in yeast cells were preferentially released by treatment with ethanol and low-pH shock. A cell wall integrity pathway seems partially involved in the hypoosmotic shock-induced release of thioredoxin. Considerable amounts of thioredoxin were present in the insoluble fractions of the cells, a portion of which was associated with lipid microdomains that are resistant to nonionic detergent at 4 degrees C. The intracellular localization of thioredoxin may influence the efficiency of its release from yeast cells with ethanol.
authors: Laboratory of Molecular Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan.
authors: Yoko
authors: Y
authors: Takeuchi
authors: Wataru
authors: W
authors: Nomura
authors: Takumi
authors: T
authors: Ohdate
authors: Shogo
authors: S
authors: Tamasu
authors: Hiroshi
authors: H
authors: Masutani
authors: Kazuo
authors: K
authors: Murata
authors: Shingo
authors: S
authors: Izawa
authors: Junji
authors: J
authors: Yodoi
authors: Yoshiharu
authors: Y
authors: Inoue
mesh_terms: D000431:Ethanol / Q000378:metabolism*; D009997:Osmotic Pressure; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D012995:Solubility; D013879:Thioredoxins / Q000378:metabolism*
chemical_list: D000431:Ethanol; D013879:Thioredoxins