Unique distribution of interstitial cells of Cajal in the feline pylorus.
The feline gastrointestinal (GI) tract is an important model for GI physiology but no immunohistochemical assessment of interstitial cells of Cajal (ICC) has been performed because of the lack of suitable antibodies. The aim of the present study was to investigate the various types of ICC and associated nerve structures in the pyloric sphincter region, by using immunohistochemistry and electron microscopy to complement functional studies. In the sphincter, ICC associated with Auerbach's plexus (ICC-AP) were markedly decreased within a region of 6-8 mm in length, thereby forming an interruption in this network of ICC-AP, which is otherwise continuous from corpus to distal ileum. In contrast, intramuscular ICC (ICC-IM) were abundant within the pylorus, especially at the inner edge of the circular muscle adjacent to the submucosa. Similar distribution patterns of nerves positive for vesicular acetylcholine transporter (VAChT), nitric oxide synthase (NOS) and substance P (SP) were encountered. Quantification showed a significantly higher number of ICC-IM and the various types of nerves in the pylorus compared with the circular muscle layers in the adjacent antrum and duodenum. Electron-microscopic studies demonstrated that ICC-IM were closely associated with enteric nerves through synapse-like junctions and with smooth muscle cells through gap junctions. Thus, for the first time, immunohistochemical studies have been successful in documenting the unique distribution of ICC in the feline pylorus. A lack of ICC-AP guarantees the distinct properties of antral and duodenal pacemaker activities. ICC-IM are associated with enteric nerves, which are concentrated in the inner portion of the circular muscle layer, being part of a unique innervation pattern of the sphincter.
Wang
Xuan-Yu
XY
Intestinal Disease Research Program and Department of Medicine, McMaster University, HSC-3N5C, 1200 Main Street West, Hamilton, ON, L8N 3Z5, Canada.
Liu
Louis W C
LW
Diamant
Nicholas E
NE
Huizinga
Jan D
JD
D000818:Animals; D002415:Cats; D004386:Duodenum / Q000294:innervation / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D007082:Ileum / Q000294:innervation / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D009130:Muscle, Smooth / Q000294:innervation / Q000378:metabolism / Q000648:ultrastructure; D009197:Myenteric Plexus / Q000378:metabolism* / Q000648:ultrastructure; D026602:Nitrergic Neurons / Q000378:metabolism* / Q000648:ultrastructure; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D011706:Pyloric Antrum / Q000294:innervation / Q000378:metabolism* / Q000648:ultrastructure; D011708:Pylorus / Q000294:innervation / Q000378:metabolism* / Q000648:ultrastructure; D013373:Substance P / Q000378:metabolism*; D013569:Synapses / Q000378:metabolism / Q000648:ultrastructure; D050494:Vesicular Acetylcholine Transport Proteins / Q000378:metabolism*
D050494:Vesicular Acetylcholine Transport Proteins; D013373:Substance P; D019001:Nitric Oxide Synthase