Lechevalieria fradiae sp. nov., a novel actinomycete isolated from soil in China.
The taxonomic position of a soil isolate, strain Z6(T), was established using a polyphasic approach. The organism showed a range of chemical and morphological properties consistent with its classification in the genus Lechevalieria. An almost complete 16S rRNA gene sequence determined for the strain was aligned with corresponding sequences of representatives of the genus Lechevalieria and related taxa using three tree-making algorithms. The organism formed a distinct phyletic line within the evolutionary radiation occupied by the genus Lechevalieria and was more closely related to the type strain of Lechevalieria aerocolonigenes than to that of Lechevalieria flava. Strain Z6(T) could be distinguished from both these strains using DNA-DNA relatedness data and by a combination of phenotypic properties. The combined genotypic and phenotypic data show that strain Z6(T) should be assigned to the genus Lechevalieria as a representative of a novel species. The name proposed for this new taxon is Lechevalieria fradiae sp. nov. The type strain is Z6(T) (=CGMCC 4.3506(T)=JCM 14205(T)).
Zhang
Jianli
J
School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, People's Republic of China.
Xie
Qiong
Q
Institute of Space Medico-Engineering, Beijing 100094, People's Republic of China.
Liu
Zhiheng
Z
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
Goodfellow
Michael
M
School of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
D000191:Actinomycetaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S