title: [Protective effects of co-immunization with SjCTPI-Hsp70 and interleukin-12 DNA vaccines against Schistosoma japonicum challenge infection in water buffalo].
abstract: OBJECTIVE
  To induce protective effect of co-immunization with S. japonicum triose-phosphate isomerase fused to heat shock protein 70 (SjCTPI-Hsp70) plasmid and interleukin-12 (IL-12) DNA vaccines against Schistosoma japonicum (Chinese strain) infection in water buffalo.
  
  
  METHODS
  Forty-five 8-10 months-old water buffalo from a nonendemic area were divided into three treatment groups each with fifteen buffalo: experimental group A (SjCTPI-Hsp70+IL-12, 300 microg), experimental group B (SjCTPI+IL-12, 300 microg), and control group C (pVAX+IL-12, 300 microg). All buffalo were immunized with a series of 3 intramuscular injections administered once every four weeks. Twenty-eight days postvaccination, water buffalo were percutaneously challenged with 1000 S. japonicum cercariae. Fecal examinations were conducted two days prior, one day prior, and on perfusion day, and the number of hatching miracidia and eggs per gram feces were recorded. Fifty-six days post-infection, the buffalo were sacrificed and perfused via the descending aorta. The recovered adult worms and eggs in liver tissue were counted.
  
  
  RESULTS
  Groups A and B showed a worm reduction rate of 51.2% and 41.5% (chi2=1.89, P>0.05)), female worm reduction of 48.9% and 44.7% (chi2=0.35,P>0.05), fecal egg reduction of 52.1% and 38.3% (chi2=3.84,P<0.05), a reduction of miracidia-hatching rate by 52.1% and 33.2% (chi2=7.30, P<0.01), and liver egg reduction of 61.5% and 42.0% (chi2=7.61 , P<0.01), respectively.
  
  
  CONCLUSIONS
  Co-immunization with SjCTPI-Hsp70 and IL-12 DNA vaccines induces protective immunity against S. japonicum in water buffalo.
authors: Hunan Institute of Parasitic Disease, Yueyang 414000, China.
authors: Xin-Ling
authors: XL
authors: Yu
authors: Yong-Kang
authors: YK
authors: He
authors: Tie
authors: T
authors: Xiong
authors: Ya-Qin
authors: YQ
authors: Zhao
authors: Meng-Zhi
authors: MZ
authors: Shi
authors: Jie
authors: J
authors: Zhou
authors: Zong-Chuan
authors: ZC
authors: Liu
authors: Xin-Song
authors: XS
authors: Luo
authors: Xiao
authors: X
authors: Fu
authors: Hong-Bin
authors: HB
authors: He
authors: D A
authors: DA
authors: Harn
authors: Yue-Sheng
authors: YS
authors: Li
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000469:parasitology*; D005243:Feces / Q000469:parasitology; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D015801:Helminth Proteins / Q000235:genetics*; D018664:Interleukin-12 / Q000235:genetics; D010273:Parasitic Diseases, Animal / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control; D010957:Plasmids / Q000235:genetics; D012549:Schistosoma japonicum / Q000235:genetics / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000276:immunology* / Q000469:parasitology / Q000517:prevention & control; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology* / Q000627:therapeutic use
chemical_list: D018840:HSP70 Heat-Shock Proteins; D015801:Helminth Proteins; D019444:Vaccines, DNA; D018664:Interleukin-12; D005956:Glucose-6-Phosphate Isomerase