Analysis of mitochondrial subunit assembly into respiratory chain complexes using Blue Native polyacrylamide gel electrophoresis.
The mitochondrial respiratory chain consists of multi-subunit protein complexes embedded in the inner membrane. Although the majority of subunits are encoded by nuclear genes and are imported into mitochondria, 13 subunits in humans are encoded by mitochondrial DNA. The coordinated assembly of subunits encoded from two genomes is a poorly understood process, with assembly pathway defects being a major determinant in mitochondrial disease. In this study, we monitored the assembly of human respiratory complexes using radiolabeled, mitochondrially encoded subunits in conjunction with Blue Native polyacrylamide gel electrophoresis. The efficiency of assembly was found to differ markedly between complexes, and intermediate complexes containing newly synthesized mitochondrial DNA-encoded subunits could be observed for complexes I, III, and IV. In particular, we detected human cytochrome b as a monomer and as a component of a novel approximately 120 kDa intermediate complex at early chase times before being totally assembled into mature complex III. Furthermore, we show that this approach is highly suited for the rapid detection of respiratory complex assembly defects in fibroblasts from patients with mitochondrial disease and, thus, has potential diagnostic applications.
McKenzie
Matthew
M
Department of Biochemistry, La Trobe University, Melbourne, VIC 3086, Australia. m.mckenzie@latrobe.edu.au
Lazarou
Michael
M
Thorburn
David R
DR
Ryan
Michael T
MT
D002478:Cells, Cultured; D004272:DNA, Mitochondrial / Q000502:physiology; D004579:Electron Transport / Q000502:physiology; D042967:Electron Transport Complex I / Q000032:analysis* / Q000737:chemistry; D014450:Electron Transport Complex III / Q000032:analysis* / Q000737:chemistry; D003576:Electron Transport Complex IV / Q000032:analysis* / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D007202:Indicators and Reagents; D008928:Mitochondria / Q000201:enzymology*; D012394:Rosaniline Dyes; D013194:Staining and Labeling
D004272:DNA, Mitochondrial; D007202:Indicators and Reagents; D012394:Rosaniline Dyes; C048139:Coomassie blue; D003576:Electron Transport Complex IV; D042967:Electron Transport Complex I; D014450:Electron Transport Complex III