title: Ha-DEF1, a sunflower defensin, induces cell death in Orobanche parasitic plants.
abstract: Plant defensins are small basic peptides of 5-10 kDa and most of them exhibit antifungal activity. In a sunflower resistant to broomrape, among the three defensin encoding cDNA identified, SF18, SD2 and HaDef1, only HaDef1 presented a preferential root expression pattern and was induced upon infection by the root parasitic plant Orobanche cumana. The amino acid sequence deduced from HaDef1 coding sequence was composed of an endoplasmic reticulum signal sequence of 28 amino acids, a standard defensin domain of 50 amino-acid residues and an unusual C-terminal domain of 30 amino acids with a net positive charge. A 5.8 kDa recombinant mature Ha-DEF1 corresponding to the defensin domain was produced in Escherichia coli and was purified by means of a two-step chromatography procedure, Immobilized Metal Affinity Chromatography (IMAC) and Ion Exchange Chromatography. Investigation of in vitro antifungal activity of Ha-DEF1 showed a strong inhibition on Saccharomyces cerevisiae growth linked to a membrane permeabilization, and a morphogenetic activity on Alternaria brassicicola germ tube development, as already reported for some other plant defensins. Bioassays also revealed that Ha-DEF1 rapidly induced browning symptoms at the radicle apex of Orobanche seedlings but not of another parasitic plant, Striga hermonthica, nor of Arabidopsis thaliana. FDA vital staining showed that these browning areas corresponded to dead cells. These results demonstrate for the first time a lethal effect of defensins on plant cells. The potent mode of action of defensin in Orobanche cell death and the possible involvement in sunflower resistance are discussed.
authors: LBPV, EA1157, Faculté des Sciences et Techniques, Université de Nantes, Nantes Atlantique Universités, 2, rue de la Houssinière, Bât. 8, Nantes 44000, France.
authors: Axel
authors: A
authors: de Zélicourt
authors: Patricia
authors: P
authors: Letousey
authors: Séverine
authors: S
authors: Thoiron
authors: Claire
authors: C
authors: Campion
authors: Philippe
authors: P
authors: Simoneau
authors: Khalil
authors: K
authors: Elmorjani
authors: Didier
authors: D
authors: Marion
authors: Philippe
authors: P
authors: Simier
authors: Philippe
authors: P
authors: Delavault
mesh_terms: D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000494:pharmacology; D001681:Biological Assay; D016923:Cell Death / Q000187:drug effects; D023082:Defensins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006368:Helianthus / Q000235:genetics / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D031672:Orobanche / Q000166:cytology*; D010455:Peptides / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D036226:Seedlings / Q000166:cytology / Q000187:drug effects
chemical_list: D000935:Antifungal Agents; D023082:Defensins; D010455:Peptides; D011994:Recombinant Proteins