title: Immunohistochemical study of experimental malignant catarrhal fever in rabbits.
abstract: Malignant catarrhal fever (MCF) is an often-fatal lymphoproliferative disease of a variety of ungulates that occurs worldwide. It is caused by either of the highly related but distinct gammaherpesviruses alcelaphine herpesvirus-1 (AlHV-1, wildebeest reservoir) or ovine herpesvirus-2 (OvHV-2, sheep reservoir). MCF in rabbits is an excellent model as it closely resembles the disease in susceptible ungulates that include cattle, deer and bison. In this study, newly available and previously characterized monoclonal antibodies specific for rabbit leucocyte differentiation molecules were used to perform a detailed immunohistochemical examination of both AlHV-1 MCF and OvHV-2 MCF in rabbits. Differences in the MCF caused by the two viruses included: less tissue necrosis and more lymphoid cell accumulations in AlHV-1 MCF compared with OvHV-2 MCF, and in particular marked tissue necrosis in the mesenteric lymph node, appendix and liver of OvHV-2-infected animals when compared with either other tissues in OvHV-2 MCF or AlHV-1 MCF lesions in any tissue. In both AlHV-1 MCF and OvHV-2 MCF, lymphoid cell accumulations in lymphoid and non-lymphoid tissues consisted mainly of T-cells with a corresponding absence of B-cells. CD8(+) T-cells accounted for a proportion of these in the non-lymphoid tissues, but there was evidence for the accumulation of an unidentified T-cell subset/subsets as well. This study extends our understanding of the mechanisms of immuno-pathogenesis of MCF.
authors: Division of Virology, Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian EH26 0PZ, Scotland, UK.
authors: I E
authors: IE
authors: Anderson
authors: D
authors: D
authors: Buxton
authors: I
authors: I
authors: Campbell
authors: G
authors: G
authors: Russell
authors: W C
authors: WC
authors: Davis
authors: M J
authors: MJ
authors: Hamilton
authors: D M
authors: DM
authors: Haig
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001065:Appendix / Q000378:metabolism / Q000473:pathology; D015415:Biomarkers / Q000378:metabolism; D004195:Disease Models, Animal; D005434:Flow Cytometry; D006965:Hyperplasia / Q000378:metabolism / Q000473:pathology; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008221:Lymphoid Tissue / Q000378:metabolism / Q000473:pathology; D008304:Malignant Catarrh / Q000378:metabolism / Q000473:pathology* / Q000821:virology; D009336:Necrosis / Q000378:metabolism / Q000473:pathology; D011817:Rabbits; D018144:Rhadinovirus / Q000276:immunology*; D013601:T-Lymphocytes / Q000378:metabolism / Q000473:pathology
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers