A novel murine model of pharyngeal candidiasis with local symptoms characteristic of pharyngeal thrush produced by using an inhaled corticosteroid.
We established a novel murine model of pharyngeal candidiasis maintaining stable yeast population and local symptoms characteristic of pharyngeal thrush. The persistent Candida-infection was prolonged by inhalation of beclomethasone dipropionate corticosteroid. The severity of infection lesions was evaluated by determining viable cell number of Candia albicans and scores representing symptomatic curd-like white patch on pharyngeal tissue. The utility of this model was shown by the disappearance of lesions and fungal cells after treatment with fluconazole (FLCZ). The model would be useful for evaluating new chemotherapeutic or immunotherapeutic approaches against pharyngeal candidiasis, as well as in pathological studies.
Hu
Weimin
W
Teikyo University, Institute of Medical Mycology, Hachioji-shi, Tokyo, Japan. huwm-teikyo@umin.ac.jp
Ninomiya
Kentaro
K
Ishibashi
Hiroko
H
Maruyama
Naho
N
Oshima
Haruyuki
H
Yamaguchi
Hideyo
H
Abe
Shigeru
S
D000280:Administration, Inhalation; D000818:Animals; D000935:Antifungal Agents / Q000627:therapeutic use; D001507:Beclomethasone / Q000008:administration & dosage*; D002176:Candida albicans / Q000254:growth & development; D002180:Candidiasis, Oral / Q000188:drug therapy / Q000382:microbiology; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D005260:Female; D015725:Fluconazole / Q000627:therapeutic use; D006651:Histocytochemistry; D007166:Immunosuppressive Agents / Q000008:administration & dosage*; D051379:Mice; D008813:Mice, Inbred ICR; D010614:Pharynx / Q000382:microbiology / Q000473:pathology
D000935:Antifungal Agents; D007166:Immunosuppressive Agents; D015725:Fluconazole; D001507:Beclomethasone