title: Nucleotide sequence of the gyrA gene of Arcobacter species and characterization of human ciprofloxacin-resistant clinical isolates.
abstract: The nucleotide sequence of the gyrA gene of Arcobacter butzleri, Arcobacter cryaerophilus, Arcobacter cibarius, and Arcobacter skirrowii was determined. The deduced GyrA proteins are closely related to those of Wolinella succinogenes and Helicobacter pullorum, whereas those of Campylobacter species showed less sequence identity. The phylogenetic analysis of GyrA sequences provides a result similar to 16S rRNA gene sequence phylogenetic analysis and allows the discrimination among A. butzleri species. In addition, a Thr-->Ile mutation at amino acid 85 in the quinolone resistance-determining region was associated with ciprofloxacin resistance for two A. butzleri and one A. cryaerophilus ciprofloxacin-resistant strains.
authors: Université Victor Segalen Bordeaux 2, Centre National de Référence des Campylobacters et Helicobacters, Laboratoire de Bacteriologie, Bordeaux, France.
authors: Khalil
authors: K
authors: Abdelbaqi
authors: Armelle
authors: A
authors: Ménard
authors: Valérie
authors: V
authors: Prouzet-Mauleon
authors: Frédéric
authors: F
authors: Bringaud
authors: Philippe
authors: P
authors: Lehours
authors: Francis
authors: F
authors: Mégraud
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D020640:Arcobacter / Q000145:classification / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D002167:Campylobacter / Q000235:genetics; D002939:Ciprofloxacin / Q000494:pharmacology*; D027081:DNA Gyrase / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D016998:Helicobacter / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D016968:Wolinella / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D002939:Ciprofloxacin; D027081:DNA Gyrase