title: Specificity of immunomodulator secretion in urinary samples in response to infection by alpha-hemolysin and CNF1 bearing uropathogenic Escherichia coli.
abstract: Escherichia coli are the most common etiological agents of urinary tract infections (UTIs). Uropathogenic E. coli (UPECs) produce specific toxins including the cytotoxic necrotizing factor-1 (CNF1) and the alpha-hemolysin (alpha-Hly). CNF1 triggers, through Rho protein activation, a specific gene response of host cells, which results in the production for instance of interleukin-8 (IL-8), monocyte chemoattractant protein-1 (MCP-1) and the macrophage inflammatory protein-3alpha (MIP-3alpha). The alpha hemolysin alpha-Hly also triggers the production of inflammatory mediators. Cnf1 is always associated with alpha-hly in a pathogenicity island conserved among UPECs. Using two complementary approaches we have investigated whether alpha-hly and cnf1 bearing UPECs are associated with a specific type of UTI both in term of pathology and host response. Here we report that UPECs bearing alpha-hly/cnf1 have a prevalence of 50% in UPECs isolated from hemorrhagic UTIs, as compared to 30% in the overall UPEC population. In addition, we observed that MCP-1, and IL-8 to a lower extent, is produced in urine at higher concentrations in UTIs caused by UPECs carrying alpha-hly/cnf1.
authors: INSERM, U627, Faculté de Médecine, 28 Avenue de Valombrose, F-06107 Nice Cedex 2, France.
authors: Jean-Michel
authors: JM
authors: Real
authors: Patrick
authors: P
authors: Munro
authors: Caroline
authors: C
authors: Buisson-Touati
authors: Emmanuel
authors: E
authors: Lemichez
authors: Patrice
authors: P
authors: Boquet
authors: Luce
authors: L
authors: Landraud
mesh_terms: D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism*; D018932:Chemokine CCL2 / Q000378:metabolism / Q000652:urine*; D054418:Chemokine CCL20; D019742:Chemokines, CC / Q000378:metabolism / Q000652:urine*; D004926:Escherichia coli / Q000276:immunology* / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000235:genetics / Q000276:immunology / Q000382:microbiology* / Q000652:urine*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D006262:Health; D006460:Hemolysin Proteins / Q000378:metabolism*; D006470:Hemorrhage / Q000235:genetics / Q000382:microbiology / Q000473:pathology; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism / Q000652:urine*; D019402:Macrophage Inflammatory Proteins / Q000378:metabolism / Q000652:urine*
chemical_list: D001427:Bacterial Toxins; C111835:CCL2 protein, human; C108355:CCL20 protein, human; D018932:Chemokine CCL2; D054418:Chemokine CCL20; D019742:Chemokines, CC; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; D016209:Interleukin-8; D019402:Macrophage Inflammatory Proteins; C060296:cytotoxic necrotizing factor type 1