[Clinicopathologic study of tumors of intermediate trophoblasts].
OBJECTIVE
To study the clinicopathologic features and immunophenotype of placental site trophoblastic tumor (PSTT) and epithelioid trophoblastic tumor (ETT).


METHODS
During the period from 1959 to 2005, a total of 1012 cases of gestational trophoblastic disease were diagnosed in Beijing Obstetrics and Gynecology Hospital. Six cases of PSTT and a case of ETT were retrieved from the archives of Beijing Obstetrics and Gynecology Hospital. Immunohistochemical study for cytokeratin 18, human chorionic gonadotropin (hCG), human placental lactogen (hPL), Mel-CAM (CD146), placental-like alkaline phosphatase (PLAP), epithelial membrane antigen (EMA), inhibin-alpha and proliferative cell nuclear antigen (PCNA) were performed. The morphologic features and immunohistochemical findings were compared with those of the controlled group which consisted of 20 cases of early gestational villi with decidua basalis and 20 cases of hydatidiform moles with implantation site.


RESULTS
The mean age of patients with PSTT was 32.4, while the age of patients with ETT was 36. Major clinical findings included irregular vaginal bleeding and amenorrhea. Preoperative serum hCG level varied from normal to moderately elevated. Serum testosterone level was raised in 1 case. Uterine curettage could achieve an accurate pathologic diagnosis in 60% of cases. ETT involved mainly the lower uterine segment and endocervix. Histologically, PSTT cells permeated between the myometrial fibers and vessels either individually or connecting in cords or sheets in a manner reminiscent of the implantation site reaction. ETT composed of a relatively uniform population of mononuclear trophoblastic cells, clumping together in nests as the cell islets associating with eosinophilic, fibrillary and hyaline material and necrotic debris, forming a "geographic map" like pattern. Immunohistochemical study for hPL, hCG, Mel-CAM (CD146) and PLAP was most helpful for the differential diagnosis. The duration of follow-up varied from 14 months to 19 years. One case of PSTT developed metastasis in pancreas, 5 months after the operation. The remaining patients survived without tumor recurrence.


CONCLUSIONS
PSTT is a tumor of implantation site intermediate trophoblasts while ETT differentiates towards chorionic-type intermediate trophoblasts. The different pathologic features and immunophenotype observed were closely related with the difference in tumor cell differentiation. An accurate pathologic diagnosis of the uterine curettage material is important for the clinical management. According to the limited follow-up data available, the clinical behavior of ETT is seemed similar to that of PSTT.
Li
Bao-zhu
BZ
Department of Pathology, Beijing Obstetrics and Gynecology Hospital, Beijing 100006, China.
Zhu
Li
L
Duan
Wei
W
D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism; D051929:CD146 Antigen / Q000378:metabolism; D006063:Chorionic Gonadotropin / Q000378:metabolism; D003937:Diagnosis, Differential; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007044:Hysterectomy / Q000379:methods; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism; D008875:Middle Aged; D010928:Placental Lactogen / Q000378:metabolism; D011247:Pregnancy; D011379:Prognosis; D014328:Trophoblastic Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D018245:Trophoblastic Tumor, Placental Site / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D014594:Uterine Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000601:surgery
D051929:CD146 Antigen; D006063:Chorionic Gonadotropin; D007527:Isoenzymes; C497902:MCAM protein, human; C055791:germ-cell AP isoenzyme; D010928:Placental Lactogen; D000469:Alkaline Phosphatase