Regulation of B-lymphocyte activation by the PH domain adaptor protein Bam32/DAPP1.
PI3Ks (phosphoinositide 3-kinases) play critical roles in BCR (B-cell receptor) signalling via the generation of 3-phosphoinositide second messengers. Recruitment of PH domain (pleckstrin homology domain)-containing signal transduction proteins to the plasma membrane through binding to 3-phosphoinositide second messengers represents a major effector mechanism for PI3Ks. Here, we review data on the PH domain-containing adaptor protein Bam32 (B-cell adaptor molecule of 32 kDa)/DAPP1 (dual adaptor for phosphotyrosine and 3-phosphoinositides 1), focusing on its functions in B-lymphocyte activation. Present results support the view that Bam32/DAPP1 mediates multiple PI3K-dependent responses in B-cells through membrane-proximal mechanisms involving Src kinases, Rac1, F-actin and mitogen-activated protein kinases, resulting in selective effects on BCR-mediated proliferation, antigen presentation and generation of antibody responses.
Marshall
A J
AJ
Department of Immunology, University of Manitoba, Winnipeg, MB, Canada. marshall@ms.umanitoba.ca
Zhang
T
T
Al-Alwan
M
M
D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology*; D002462:Cell Membrane / Q000378:metabolism; D003599:Cytoskeleton / Q000502:physiology; D006801:Humans; D008074:Lipoproteins / Q000378:metabolism*; D008213:Lymphocyte Activation; D051379:Mice; D010766:Phosphorylation; D011947:Receptors, Antigen, B-Cell / Q000276:immunology; D011993:Recombinant Fusion Proteins; D015398:Signal Transduction
D048868:Adaptor Proteins, Signal Transducing; C486555:DAPP1 protein, human; C403524:Dapp1 protein, mouse; D008074:Lipoproteins; D011947:Receptors, Antigen, B-Cell; D011993:Recombinant Fusion Proteins