title: Laminar shear stress up-regulates the expression of stearoyl-CoA desaturase-1 in vascular endothelial cells.
abstract: OBJECTIVE
  Laminar shear stress plays critical roles in vascular homeostasis and exerts various metabolic effects on endothelial cells (ECs). Stearoyl-CoA desaturase-1 (SCD1), which catalyzes the biosynthesis of monounsaturated fatty acids, affects the lipid composition and fluidity of the cell membrane. Thus, we examined the effect of laminar flow on SCD1 expression in ECs.
  
  
  METHODS
  A flow chamber was used to impose a laminar shear stress on a confluent monolayer of human vascular ECs. The expression of SCD1 was examined using real-time RT-PCR and Northern and Western blotting. Immunohistochemical staining was used to assess the expression of SCD1 in Sprague-Dawley rat arteries, including the sites of arterial bifurcation.
  
  
  RESULTS
  Laminar shear stress (12 dyn/cm2, 12 h) markedly increased the gene expression of SCD1 in ECs. The flow-induced SCD1 expression was attenuated by peroxisome proliferator-activated receptor (PPAR)-gamma antagonists both in vitro and in vivo. Troglitazone and rosiglitazone significantly increased the gene expression of SCD1. Furthermore, overexpression of a constitutively active PPARgamma induced the expression of SCD1 in ECs. Immunohistochemical study of cross-sections from rat celiac arteries revealed that endothelial expression of SCD1 was substantially higher on the medial division apex, where the shear stress is high and more laminar, than the lateral aspect, where the shear stress is low and unsteady.
  
  
  CONCLUSIONS
  These in vitro and in vivo results demonstrate that laminar flow increased the expression of SCD1 in endothelium through a PPARgamma-specific mechanism, which may contribute to the shear stress-mediated protective roles in ECs.
authors: Institute of Cardiovascular Science and Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Peking University, Beijing, 100083, PR China.
authors: Xiaomei
authors: X
authors: Qin
authors: Jianwei
authors: J
authors: Tian
authors: Peng
authors: P
authors: Zhang
authors: Yanbo
authors: Y
authors: Fan
authors: Li
authors: L
authors: Chen
authors: Youfei
authors: Y
authors: Guan
authors: Yi
authors: Y
authors: Fu
authors: Yi
authors: Y
authors: Zhu
authors: Shu
authors: S
authors: Chien
authors: Nanping
authors: N
authors: Wang
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002445:Celiac Artery / Q000201:enzymology; D002465:Cell Movement; D002478:Cells, Cultured; D002839:Chromans / Q000494:pharmacology; D042783:Endothelial Cells / Q000201:enzymology*; D005786:Gene Expression Regulation; D006801:Humans; D007150:Immunohistochemistry; D047495:PPAR gamma / Q000037:antagonists & inhibitors / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012039:Regional Blood Flow; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000077154:Rosiglitazone; D013230:Stearoyl-CoA Desaturase / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D013314:Stress, Mechanical; D045162:Thiazolidinediones / Q000494:pharmacology; D000077288:Troglitazone
chemical_list: D002839:Chromans; D047495:PPAR gamma; D012333:RNA, Messenger; D045162:Thiazolidinediones; D000077154:Rosiglitazone; D013230:Stearoyl-CoA Desaturase; D000077288:Troglitazone