title: Interactions of collagen types I and II with chondroitin sulfates A-C and their effect on osteoblast adhesion.
abstract: Collagen has found use as a scaffold material for tissue engineering as well as a coating material for implants. The main aim of this study was to compare the ability of the collagen types I and II to bind preparations of the chondroitin sulfate types A-C (CS A, CS B, CS C). In addition, the effect of the three CS preparations on the extent of collagen incorporated into fibrils and the morphology of collagen fibrils was investigated, as was the influence of collagen fibril coatings containing CS A-C on titanium surfaces on the adhesion of primary rat osteoblasts. Fibrils of both collagen types bound a higher mass of CS C than CS B and a greater mass of CS B than CS A per milligram of fibrils formed. Fibrils of collagen type II bound a higher mass of CS B and C than collagen I fibrils. The proportion of collagen incorporated into fibrils decreased with increasing CS A and CS C concentration but not with increasing CS B concentration. All three CS preparations caused collagen I and II fibrils to become thinner. CS A and CS B but not CS C appeared to stimulate the formation of focal adhesions by osteoblasts after incubation for 2 hours. These results could be of importance when selecting collagen type or CS type as materials for implant coatings or tissue engineering scaffolds.
authors: Institute of Material Science, Technische Universität Dresden, Budapester Strasse 27, 01069 Dresden, Germany. Timothy.Douglas@mailbox.tu-dresden.de
authors: Timothy
authors: T
authors: Douglas
authors: Sascha
authors: S
authors: Heinemann
authors: Carolin
authors: C
authors: Mietrach
authors: Ute
authors: U
authors: Hempel
authors: Susanne
authors: S
authors: Bierbaum
authors: Dieter
authors: D
authors: Scharnweber
authors: Hartmut
authors: H
authors: Worch
mesh_terms: D000818:Animals; D001665:Binding Sites; D002236:Carbohydrate Conformation; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000737:chemistry / Q000494:pharmacology; D024042:Collagen Type I / Q000737:chemistry / Q000494:pharmacology; D024043:Collagen Type II / Q000737:chemistry / Q000494:pharmacology; D008969:Molecular Sequence Data; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D011921:Rats, Inbred WKY; D013329:Structure-Activity Relationship
chemical_list: D024042:Collagen Type I; D024043:Collagen Type II; D002809:Chondroitin Sulfates