title: Production of a new tissue-engineered adipose substitute from human adipose-derived stromal cells.
abstract: Adipose tissue is an accessible and abundant source of mesenchymal stem cells for soft-tissue reconstruction. In an attempt to create a novel, entirely autologous tissue-engineered adipose substitute, we extracted human stromal cells from either lipoaspirated or resected fat, and assessed their capacity to produce a three-dimensional adipose tissue using an adapted "self-assembly" culture methodology. This strategy involved a concomitant induction of adipogenic differentiation whilst ascorbic acid supplementation stimulated the stromal cells to produce and organize their own "biomaterial" in the form of extracellular matrix, forming manipulatable sheets that are then assembled into thicker reconstructed adipose tissues. When compared to resected fat, lipoaspiration-derived cells featured an increased adipogenic potential and the enhanced ability to recreate an adipose substitute in vitro. When viewed by scanning electron microscopy, the appearance of these reconstructed adipose tissues was strikingly similar to subcutaneous fat. Furthermore, these substitutes secreted adipokines and mediated beta-adrenergic receptor-stimulated lipolysis, hence reproducing known major biological functions of white adipose tissue. Therefore, our cell-based tissue engineering strategy led to the production of a functional and entirely natural reconstructed adipose tissue, which offers the potential to be used for specific in vitro applications as well as for autologous soft-tissue reconstruction.
authors: Laboratoire d'Organogénèse Expérimentale (LOEX), Centre Hospitalier Affilié Universitaire de Québec, Hôpital du Saint-Sacrement, 1050 chemin Sainte-Foy, Québec., Canada.
authors: Mélanie
authors: M
authors: Vermette
authors: Valérie
authors: V
authors: Trottier
authors: Vincent
authors: V
authors: Ménard
authors: Lucie
authors: L
authors: Saint-Pierre
authors: Alphonse
authors: A
authors: Roy
authors: Julie
authors: J
authors: Fradette
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D000328:Adult; D001205:Ascorbic Acid / Q000494:pharmacology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology; D020738:Leptin / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D017154:Stromal Cells / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D014815:Vitamins / Q000494:pharmacology
chemical_list: D020738:Leptin; D014815:Vitamins; D007545:Isoproterenol; D001205:Ascorbic Acid