Microarray analysis of IL-1beta-stimulated chemokine genes in synovial fibroblasts from human TMJ.
BACKGROUND
Interleukin (IL)-1beta is thought to play a key role in several pathologic conditions of the temporomandibular joint (TMJ). Gene expression profile of synovial fibroblasts stimulated with IL-1beta was studied by oligonucleotide microarray analysis to elucidate candidate genes associated with intracapsular pathologic conditions of TMJ.


METHODS
RNA was isolated from synovial fibroblasts from five patients after IL-1beta treatment. Gene expression profiling was performed with a GeneChip. Changes in gene expression were determined by comparing IL-1beta-treated cells with untreated cells.


RESULTS
A total of 121 genes showed a greater than threefold difference in average intensity between untreated and IL-1beta-treated synovial fibroblasts in five experiments. Five chemokines were among the 10 most upregulated genes, and the most upregulated gene was CCL20. The 121 IL-1beta-responsive genes included 12 chemokines whose mRNA levels were confirmed by real-time PCR.


CONCLUSIONS
These data should provided useful information about the pathologic conditions of TMJ, especially in support of diagnosis and therapeutic approaches to TMJ.
Ogura
Naomi
N
Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
Akutsu
Miwa
M
Tobe
Makiko
M
Sakamaki
Hiroyuki
H
Abiko
Yoshimitsu
Y
Kondoh
Toshirou
T
D000293:Adolescent; D000328:Adult; D018925:Chemokines / Q000096:biosynthesis* / Q000235:genetics; D002633:Chemotaxis / Q000235:genetics; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D020869:Gene Expression Profiling; D006801:Humans; D053583:Interleukin-1beta / Q000502:physiology*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D013583:Synovial Membrane / Q000166:cytology / Q000378:metabolism; D013585:Synovitis / Q000235:genetics / Q000378:metabolism*; D013704:Temporomandibular Joint / Q000378:metabolism*; D013705:Temporomandibular Joint Disorders / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
D018925:Chemokines; D053583:Interleukin-1beta