Determination of residues of tricaine in fish using liquid chromatography tandem mass spectrometry.
A liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the determination of residues of the anaesthetic tricaine mesilate (MS222) in fish tissues is described. Residues were extracted from homogenized tissues with McIllvaine buffer/methanol and purified over a C18 solid-phase extraction column followed by LC-MS/MS analysis. In the multiple-reaction monitoring mode of the mass spectrometer, chromatograms were recorded by monitoring the m/z 166-->m/z 138 and m/z 166-->m/z 94 transitions for quantification and confirmation of the residues in the finfish matrix, respectively. Recoveries were in the range of 67%+/-10% (n=6) for tilapia at 2 microg kg(-1), 95%+/-7% (n=6) at 2 microg kg(-1) in salmon and 92%+/-3% (n=5) for trout at 2.5 microg kg(-1). The limits of detection were 0.5, 0.6 and 0.6 microg kg(-1) in trout, salmon and tilapia, respectively. No residues of tricaine were found in eight sampled aquacultured fish (salmon and trout) bought from the local market.
Scherpenisse
Peter
P
Veterinary Public Health Division, Institute for Risk Assessment Sciences, Utrecht University, P.O. Box 80.175, NL-3508 TD Utrecht, The Netherlands.
Bergwerff
Aldert A
AA
D062365:Aminobenzoates / Q000032:analysis*; D000777:Anesthetics / Q000032:analysis*; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D005399:Fishes; D005504:Food Analysis / Q000379:methods; D005506:Food Contamination; D009132:Muscles / Q000187:drug effects / Q000378:metabolism; D011786:Quality Control; D012474:Salmon; D013045:Species Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D017210:Tilapia; D014337:Trout
D062365:Aminobenzoates; D000777:Anesthetics; C003636:tricaine