Regulation of the CAMP gene by 1,25(OH)2D3 in various tissues.
The induction of antimicrobial peptides such as the human cathelicidin, CAMP/hCAP18, by 1,25(OH)(2)D(3) provides a very exciting therapeutic approach in boosting immunity against infectious diseases. To explore the range of cell types and expand the number of cell models for studying the regulation of CAMP gene expression by 1,25(OH)(2)D(3), we treated cell lines from various tissue types and determined CAMP gene expression. Also, we tested additional compounds together with 1,25(OH)(2)D(3) to look for possible cooperative activation of the gene. We identified 1,25(OH)(2)D(3)-mediated induction of the CAMP gene in B-cell lymphomas, prostate and endometrial cancer lines and found cooperative activation with the histone deacetylase inhibitor sodium butyrate. The data suggest that regulation of CAMP by 1,25(OH)(2)D(3) is potentially important in a wide range of tissues.
Gombart
Adrian F
AF
Cedars-Sinai Medical Center, Department of Medicine, Division of Hematology/Oncology, Burns & Allen Research Institute, David Geffen School of Medicine at UCLA, 8700 Beverly Blvd, Los Angeles, CA 90048, USA. gombarta@csmc.edu
O'Kelly
James
J
Saito
Tsuyako
T
Koeffler
H Phillip
HP
D023181:Antimicrobial Cationic Peptides / Q000235:genetics*; D020148:Butyric Acid / Q000494:pharmacology; D002117:Calcitriol / Q000494:pharmacology*; D054804:Cathelicidins; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics
D023181:Antimicrobial Cationic Peptides; D054804:Cathelicidins; D012333:RNA, Messenger; D020148:Butyric Acid; D002117:Calcitriol