title: [Expression of TEIF protein in soft tissue tumors and its significance].
abstract: OBJECTIVE
  To evaluate the expression of TEIF protein in human tumors of soft tissue and its significance.
  
  
  METHODS
  Anti-TEIF polyclonal antibody was generated by immunization of E.coli expressed His-TEIF protein. The expression of TEIF in 166 cases of sarcomas and 28 case benign tumors or tumor-like lesions of soft tissue arranged in tissue chip was analyzed by immunohistochemistry.
  
  
  RESULTS
  Polyclonal antibody obtained from immunized rabbit was verified in Western blot to prove its specific reactivity with native TEIF protein. The immunohistochemical staining of TEIF showed that about 58% (97/166) of sarcomas were positive and significantly different from that of benign tumors or tumor-like lesions (11%, 3/28). The positive staining was predominantly in synovial sarcoma 94% (16/17), primitive neuroectodermal tumor (PNET) 91% (21/23), both of which were significantly higher than 43% (6/14) of dermatofibrosarcoma protuberans, 38% (6/16) of myxofibrosarcoma, 36% (8/22) of malignant peripheral nerve sheath tumor, 32% (6/19) of liposarcoma, (P < 0.05, respectively), but not higher than 75% (15/20) of malignant fibrous histiocytoma, 70% (7/10) of rhabdomyosarcoma or 64% (9/14) of leiomyosarcoma. Meanwhile, strong positive staining of TEIF (>or= 2+) was frequently observed in PNET (83%, 19/23) and synovial sarcoma (76%, 13/17). With respect to FNCLCC grading, 19 cases of grade I sarcoma TEIF was 32% (6/19) and strong positive was 11% (2/19), 44 cases of grade II sarcoma was 48% (21/44) and 32% (14/44), and 70 of grade III was 84% (59/70) and 70% (49/70). The rate of either positive or strong positive in grade III sarcoma was significantly different from that of either grade I or II (P < 0.05), but no difference between the latter two groups (P > 0.05).
  
  
  CONCLUSIONS
  TEIF protein could be detected in large part of soft tissue sarcomas, and it not only over-expressed in most of PNET, synovial sarcomas, but also correlated with histological grading.
authors: Department of Pathology, Health Science Center of Peking University, Beijing 100083, China.
authors: Yi-lei
authors: YL
authors: Gong
authors: Ting
authors: T
authors: Li
authors: Hua
authors: H
authors: Guo
authors: Ying
authors: Y
authors: Sun
authors: Ying-kai
authors: YK
authors: Chi
authors: Yun
authors: Y
authors: Ling
authors: Qi
authors: Q
authors: Shen
authors: Hai-jing
authors: HJ
authors: Liu
authors: Lin
authors: L
authors: Hou
authors: Bo
authors: B
authors: Zhang
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002648:Child; D002675:Child, Preschool; D004268:DNA-Binding Proteins; D005260:Female; D006367:HeLa Cells; D051677:Histiocytoma, Malignant Fibrous / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D007889:Leiomyoma / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D018242:Neuroectodermal Tumors, Primitive / Q000378:metabolism / Q000473:pathology; D012208:Rhabdomyosarcoma / Q000378:metabolism / Q000473:pathology; D012509:Sarcoma / Q000378:metabolism* / Q000473:pathology; D013584:Sarcoma, Synovial / Q000378:metabolism / Q000473:pathology; D012983:Soft Tissue Neoplasms / Q000378:metabolism* / Q000473:pathology; D046888:Tissue Array Analysis; D014157:Transcription Factors / Q000096:biosynthesis*; D055815:Young Adult
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D004268:DNA-Binding Proteins; C464929:SCYL1 protein, human; D014157:Transcription Factors