The association of CYP2C9 gene polymorphisms with colorectal carcinoma in Han Chinese.
BACKGROUND
Cytochrome P450 (CYP) 2C9 is an important enzyme involved in xenobiotics metabolism. This study investigated the association of CYP2C9 gene coding region polymorphisms with colorectal cancer (CRC) in Chinese Han population.


METHODS
Four hundred and eighty-three healthy controls and 286 sporadic CRC patients participated in this study. Direct sequencing was used to identify the sequence polymorphisms.


RESULTS
We detected the significant association of 2 coding region SNPs, rs1057910 and rs1057911, of CYP2C9 with the risk of developing sporadic CRC for Han Chinese. These 2 SNPs showed a strong linkage disequilibrium (LD) (r(2)=0.97, D'=0.985). Significantly different minor allele frequencies were found for SNPs rs1057910 and rs1057911 between the cases (7% and 7.2%, respectively) and controls (3% and 2.9%, respectively) with adjusted P=0.0004 and 0.0002, respectively. Individuals heterozygous for rs1057910A/C or rs1057911A/T showed 2.589-fold (95% CI: 1.549-4.330) or 2.770-fold (95% CI 1.653-4.643) increased risk of developing sporadic CRC. We did not detect any homozygote minor allele carrier for either rs1057910 or rs1057911 in our study population. The CRC association appeared to be more evident for individuals over age 50 y, for men, and for rectum cancer site.


CONCLUSIONS
There is an association of CYP2C9 coding region polymorphisms with the risk of developing CRC in Han Chinese after genotyping cases and controls recruited from different locations in China.
Liao
Ling-Hong
LH
Department of Biology and Center for Cancer Research, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, PR China.
Zhang
Hao
H
Lai
Man-Po
MP
Lau
Kwok-Wai
KW
Lai
Albert Kai-Cheong
AK
Zhang
Jin-Hui
JH
Wang
Qi
Q
Wei
Wei
W
Chai
Jian-Hua
JH
Lung
Maria Li
ML
Tai
Susan S W
SS
Wu
Madeline
M
D000483:Alleles; D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics*; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D015179:Colorectal Neoplasms / Q000453:epidemiology / Q000235:genetics*; D065729:Cytochrome P-450 CYP2C9; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006306:Health Surveys; D006720:Homozygote; D006801:Humans; D008297:Male; D008499:Medical Records; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects
C450260:CYP2C9 protein, human; D065729:Cytochrome P-450 CYP2C9; D001189:Aryl Hydrocarbon Hydroxylases