Correlation between phosphorylation level of a hippocampal 86kDa protein and extinction of a behaviour in a model of Wernicke-Korsakoff syndrome.
The effects of chronic ethanol and thiamine deficiency, alone or associated, on hippocampal protein phosphorylation profiles ranging in molecular weight from 30 to 250kDa molecular weight, in stimulated (high K(+) concentration) and unstimulated (basal) conditions were investigated. These treatments significantly changed the phosphorylation level of an 86kDa phosphoprotein. Thiamine deficiency, but not chronic ethanol, induced a decrease in a behavioural extinction index, which is significantly correlated to the phosphorylation level of the p86 protein. These data add to and extend previous findings by our laboratory implicating the involvement of hippocampal neurotransmission components in extinction of a behaviour which involves learning of environmental spatial cues.
Pires
Rita G W
RG
Departamento de Bioquímica-Imunologia, Laboratório de Neurociência Comportamental e Molecular (LaNeC), Universidade Federal de Minas Gerais, Belo Horizonte 31270-010, Brazil.
Pereira
Sílvia R C
SR
Carvalho
Fabiana M
FM
Oliveira-Silva
Ieda F
IF
Ferraz
Vany P
VP
Ribeiro
Angela M
AM
D000818:Animals; D002492:Central Nervous System Depressants / Q000494:pharmacology; D004195:Disease Models, Animal; D000431:Ethanol / Q000494:pharmacology; D005108:Extinction, Psychological / Q000502:physiology*; D006624:Hippocampus / Q000378:metabolism*; D020915:Korsakoff Syndrome / Q000378:metabolism*; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D017208:Rats, Wistar; D013832:Thiamine Deficiency / Q000378:metabolism
D002492:Central Nervous System Depressants; D009419:Nerve Tissue Proteins; D000431:Ethanol