Reactions of 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulfonate) dianion, ABTS2-, with *OH, (SCN)2*-, and glycine or valine peroxyl radicals.
ABTS2-, 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulfonate) dianion, was used as a reference to compare the reactivity of peroxyl radicals of two amino acids, glycine and valine, in aqueous solutions at natural pH. Peroxyl radicals were produced by pulse radiolysis and the product of their reaction with ABTS2- the ABTS*- radical was observed spectrophotometrically. Experimental kinetic traces were fitted using chemical simulation. The rate constants of reactions of glycine and valine peroxyl radicals with ABTS2- were (6.0+/-0.2)x10(6) and (1.3+/-0.1)x10(5) M-1.s-1, respectively. Moreover, it was found that only 60% of glycine radicals formed upon its reaction with *OH radicals reacted with molecular oxygen to yield peroxyl radicals. Comparison of experimental data with simulations of chemical reactions in irradiated ABTS and ABTS/NaSCN solutions showed that ABTS*- forms in the reaction with *OH with a yield of 43% and rate constant of (5.4+/-0.2)x10(9) M-1.s-1 and in the reaction with (SCN)2*- with a yield of 57% and rate constant of (8.0+/-0.2)x10(8) M-1.s-1.
Gebicki
Jerzy L
JL
Institute of Applied Radiation Chemistry, Faculty of Chemistry, TU of Lodz, ul. Wroblewskiego 15, 93-590 Lodz, Poland. jlgebick@mitr.p.lodz.pl
Maciejewska
Magdalena
M
D000838:Anions / Q000737:chemistry; D052160:Benzothiazoles / Q000737:chemistry*; D005609:Free Radicals / Q000737:chemistry; D005998:Glycine / Q000737:chemistry*; D007700:Kinetics; D010545:Peroxides / Q000737:chemistry*; D013057:Spectrum Analysis; D013451:Sulfonic Acids / Q000737:chemistry*; D014633:Valine / Q000737:chemistry*
D000838:Anions; D052160:Benzothiazoles; D005609:Free Radicals; D010545:Peroxides; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D014633:Valine; D005998:Glycine