Oxidative stress alters physiological and morphological neuronal properties.
We investigated the effects of H(2)O(2)-induced oxidative stress on the delayed-rectifier current (IK(DR)), neuronal physiological and morphological properties. Measurements were obtained from hippocampal CA1 neurons in control solution and from the same neurons after exposure to oxidative stress (short- and long-term H(2)O(2) external applications at 0.1, 1, and 10 mM). With short-term (6 min) H(2)O(2) (1 mM) treatment, IK(DR) measured in the H(2)O(2)-containing solution (778 +/- 23 pA, n=20), was smaller than that measured in the control Ca(2+)-free Hepes solution (1,112 +/- 38 pA, n=20). Coenzyme Q(10) (0.1 mM) pretreatment prevented the H(2)O(2)-induced inhibition of IK(DR). With long-term (40, 80 min) H(2)O(2) (0.1, 10 mM) treatment, the neuron lost its distinctive shape (rounded up) and the neurite almost disappeared. These results suggest that oxidative stress, which inhibits IK(DR), can alter neural activity. The morphological changes caused by H(2)O(2) support the idea that oxidative stress causes intracellular damage and compromises neural function.
Hasan
Sonia M
SM
Department of Physiology, Faculty of Medicine, Kuwait University, P.O. Box 24923, Safat, 13110, Kuwait.
Joe
Mary
M
Alshuaib
Waleed B
WB
D000818:Animals; D001464:Barium / Q000378:metabolism; D003067:Coenzymes / Q000378:metabolism; D051668:Delayed Rectifier Potassium Channels / Q000378:metabolism*; D045222:Electron Transport Chain Complex Proteins / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008297:Male; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D016877:Oxidants / Q000494:pharmacology*; D018384:Oxidative Stress; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000378:metabolism; D014815:Vitamins / Q000378:metabolism
D003067:Coenzymes; D051668:Delayed Rectifier Potassium Channels; D045222:Electron Transport Chain Complex Proteins; D016877:Oxidants; D014815:Vitamins; D014451:Ubiquinone; D001464:Barium; D006861:Hydrogen Peroxide; C024989:coenzyme Q10