The antioxidant/electrophile response element motif.
Cells exposed to oxidative stress or electrophilic xenobiotics respond by transcriptionally up-regulating a battery of genes that contain a cis-acting element in their promoter region known as the antioxidant/electrophile response element (ARE). Mutational analysis of the promoter regions of ARE-containing genes led to the creation of two different models for the ARE; a core ARE (cARE: RTGACnnnGC) and an extended ARE (eARE: TMAnnRTGAYnnnGCAwwww). Using bioinformatic software we have aligned the promoter regions of several ARE-containing genes to produce two position-specific probability matrices that independently describe the cARE and eARE. These matrices can also be used to quantitatively assess putative AREs.
Nerland
Donald E
DE
Department of Pharmacology & Toxicology, Health Sciences Center, University of Louisville, Louisville, KY, USA. denerl01@louisville.edu
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001483:Base Sequence; D019295:Computational Biology / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D009154:Mutation; D011401:Promoter Regions, Genetic / Q000235:genetics*; D020218:Response Elements / Q000235:genetics*
D000975:Antioxidants