title: Chemokine and chemokine receptor analysis reveals elevated interferon-inducible protein-10 (IP)-10/CXCL10 levels and increased number of CCR5+ and CXCR3+ CD4 T cells in synovial fluid of patients with enthesitis-related arthritis (ERA).
abstract: Chemokines and chemokine receptors play a major role in homing of cells to the site of inflammation. Enthesitis-related arthritis (ERA) is a chronic inflammatory arthritis and no data are available on chemokines and their receptors in ERA. Blood (20) and synovial fluid (SF) (11) was collected from patients with ERA, and peripheral blood (PB) was collected from 12 patients with polyarticular juvenile idiopathic arthritis (JIA), nine patients with systemic onset and 18 healthy controls. Chemokines [interleukin (IL)-10/CXCL10, thymus and activation-regulated chemokine (TARC)/CCL17 and regulated upon activation normal T cell expressed and secreted (RANTES)/CCL5] were measured in serum and SF. Chemokine receptor expression was measured by flow cytometry. There was no difference in blood CD4(+) T cells bearing CCR5, CCR4 and CXCR3 in ERA and healthy controls. In paired samples the median frequency of CCR5(+) CD4(+) T cells was higher in SF compared to PB (15.8 versus 3.9%, P < 0.005), as was the frequency of CXCR3(+) T cells (21.61% versus 12.46%, P < 0.05). Median serum interferon-inducible protein-10 (IP-10)/CXCL10 levels were higher in patients with ERA compared to controls (139 versus 93 pg/ml; P < 0.05). Further median SF IP-10/CXCL10 levels were higher than the serum levels (2300 pg/ml versus 139 pg/ml; P < 0.01). Serum levels of RANTES/CCL5 were higher in patients (150 ng/ml) compared to control (99 ng/ml; P < 0.01). The SF levels were significantly lower compared to serum (P < 0.05). TARC/CCL17 levels in SF were lower than serum. There is increased homing of CCR5 and CXCR3(+) CD4 cells to the SF. Increased SF levels of IP-10/CXCL10 may be responsible for this migration in patients with ERA.
authors: Department of Immunology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India. amita@sgpgi.ac.in
authors: A
authors: A
authors: Aggarwal
authors: S
authors: S
authors: Agarwal
authors: R
authors: R
authors: Misra
mesh_terms: D000293:Adolescent; D000328:Adult; D001168:Arthritis / Q000276:immunology*; D001171:Arthritis, Juvenile / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018946:Chemokine CCL5; D054357:Chemokine CXCL10; D018925:Chemokines / Q000378:metabolism*; D019742:Chemokines, CC / Q000097:blood / Q000378:metabolism; D019743:Chemokines, CXC / Q000097:blood / Q000378:metabolism; D002648:Child; D005260:Female; D006801:Humans; D008297:Male; D019713:Receptors, CCR5 / Q000378:metabolism; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine / Q000032:analysis / Q000378:metabolism*; D013582:Synovial Fluid / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology
chemical_list: C483024:CCL5 protein, human; C515187:CXCR3 protein, human; D018946:Chemokine CCL5; D054357:Chemokine CXCL10; D018925:Chemokines; D019742:Chemokines, CC; D019743:Chemokines, CXC; D019713:Receptors, CCR5; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine