Trichostatin A induces myocardial differentiation of monkey ES cells.
Human embryonic stem (ES) cell lines are one of the possible sources of cardiac myocytes to be transplanted in patients with end-staged heart failure. However, prior to the application of human of ES cells for heart failure therapy, it is critical to validate their clinical use in large animals such as primates. Cynomolgus monkey ES cells have similar properties to human ES cells and can be used for primate studies. We demonstrate that 24-h stimulation by a histone deacetylase inhibitor, trichostatin A (TSA) facilitated myocardial differentiation of monkey ES cells with embryonic bodies that were seeded on gelatin-coated dishes. TSA-induced acetylating of histone-3/4 and expression of p300, one of the intrinsic histone acetyltransferases. Thus, such induction as well as inhibition of histone deacetylase may be involved in TSA-induced differentiation of cynomolgus monkey ES cells into cardiomyocytes.
Hosseinkhani
Mohsen
M
Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Hasegawa
Koji
K
Ono
Koh
K
Kawamura
Teruhisa
T
Takaya
Tomohide
T
Morimoto
Tatsuya
T
Wada
Hiromichi
H
Shimatsu
Akira
A
Prat
Susana Gonzalez
SG
Suemori
Hirofumi
H
Nakatsuji
Norio
N
Kita
Toru
T
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology / Q000187:drug effects*; D006877:Hydroxamic Acids / Q000494:pharmacology*; D008252:Macaca fascicularis; D009206:Myocardium / Q000166:cytology*
D006877:Hydroxamic Acids; C012589:trichostatin A