Determination of the anthraquinones aloe-emodin and aloin-A by liquid chromatography with mass spectrometric and diode array detection.
Methods using liquid chromatography/mass spectrometry (LC/MS) and LC with diode array detection (DAD) in the UV range (LC/UV) were developed for the determination of low levels of the anthraquinones aloe-emodin and aloin-A (barbaloin) in aloe-based products. The methods were used to analyze several commercial products (liquids, semisolids, and solids) for the 2 anthraquinones. The wavelengths used for quantification of aloin-A, aloe-emodin, and emodin (internal standard) by DAD were 357, 257, and 289 nm, respectively. The on-column sensitivities were 0.25 and 0.05 ng by LC/UV and 0.01 and 0.025 ng by LC/MS for aloin-A and aloe-emodin, respectively. The methods are simple and sensitive and provide reproducible results; therefore, they are suitable for the determination of these anthraquinones in various aloe-based products.
Elsohly
Mahmoud A
MA
ElSohly Laboratories Inc., 5 Industrial Park Dr, Oxford, MS 38655, USA. elsohly@elsohly.com
Gul
Waseem
W
Avula
Bharathi
B
Khan
Ikhlas A
IA
D000880:Anthraquinones; D002853:Chromatography, Liquid; D004642:Emodin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry; D013058:Mass Spectrometry; D015394:Molecular Structure; D013056:Spectrophotometry, Ultraviolet
D000880:Anthraquinones; C518327:aloe emodin; D004642:Emodin; C006457:alloin