title: Changes in expression of interleukin-6 receptors in granulosa cells during follicular atresia in pig ovaries.
abstract: More than 99% of follicles undergo a degenerative process known as "atresia" in mammalian ovaries, and only a few follicles ovulate during follicular growth and development. Follicular selection predominantly depends on granulosa cell apoptosis. To reveal the molecular mechanisms of selective follicular atresia, we examined the changes in the levels of interleukin-6 (IL-6) receptors expressed in the granulosa cells of pig ovaries. The levels of IL-6 receptor (IL-6R)-alpha mRNA and protein in granulosa cells were quantified by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting, respectively. IL-6R alpha mRNA and protein were highly expressed in the granulosa cells of progressed atretic follicles. Enzyme-linked immunosorbent assay showed that the expression of IL-6 soluble receptor (IL-6sR) protein in follicular fluid decreased during atresia. Moreover, we isolated porcine cDNA encoding an IL-6 signal transducer, gp130. Porcine gp130 (2,754 bp and 917 amino acids) was identified from a cDNA library prepared using follicular granulosa cells of pig ovaries. Porcine gp130 was highly homologous with human and murine gp130. RT-PCR analysis revealed that the level of gp130 mRNA also decreased during atresia. We presume that IL-6sR and gp130, but not IL-6R alpha, play important roles in regulation of granulosa cell survival.
authors: Research Unit for Animal Life Sciences, Animal Resource Science Center, The University of Tokyo, Kasama, Japan.
authors: Akihisa
authors: A
authors: Maeda
authors: Yasufumi
authors: Y
authors: Goto
authors: Fuko
authors: F
authors: Matsuda-Minehata
authors: Yuan
authors: Y
authors: Cheng
authors: Naoko
authors: N
authors: Inoue
authors: Noboru
authors: N
authors: Manabe
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D003001:Cloning, Molecular; D050822:Cytokine Receptor gp130 / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005496:Follicular Atresia / Q000502:physiology*; D015571:Follicular Fluid / Q000502:physiology; D015870:Gene Expression / Q000502:physiology; D006107:Granulosa Cells / Q000166:cytology / Q000502:physiology*; D053698:Interleukin-6 Receptor alpha Subunit / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D012995:Solubility; D034421:Sus scrofa / Q000235:genetics*
chemical_list: D053698:Interleukin-6 Receptor alpha Subunit; D012333:RNA, Messenger; D050822:Cytokine Receptor gp130