Legionella pneumophila infection up-regulates dendritic cell Toll-like receptor 2 (TLR2)/TLR4 expression and key maturation markers.
Dendritic cells (DCs) have a critical role in linking innate to adaptive immunity, and this transition is regulated by the up-regulation of costimulatory and major histocompatibility complex (MHC) molecules as well as Toll-like receptors. These changes in DCs have been observed to occur following microbial infection, and in the present study, we examined the effect of Legionella pneumophila infection on the expression of these DC markers. We showed that bone marrow-derived DC cultures from BALB/c mice infected with live L. pneumophila resulted in the up-regulation of Toll-like receptors 2 and 4 and the activation of CD40, CD86, and MHC class I/II molecules.
Rogers
James
J
Department of Molecular Medicine, University of South Florida, 12901 Bruce B. Downs Boulevard, Tampa, FL 33612-4799, USA.
Hakki
Amal
A
Perkins
Izabella
I
Newton
Catherine
C
Widen
Ray
R
Burdash
Nicholas
N
Klein
Thomas
T
Friedman
Herman
H
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D003713:Dendritic Cells / Q000378:metabolism / Q000382:microbiology*; D016952:Legionella pneumophila / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D051195:Toll-Like Receptor 2 / Q000502:physiology*; D051197:Toll-Like Receptor 4 / Q000378:metabolism*; D015854:Up-Regulation
D015415:Biomarkers; C495345:TLR4 protein, human; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4