Application of an electrometric method for measurement of blood cholinesterase activities in sheep, goats and cattle treated with organophosphate insecticides.
A modified electrometric cholinesterase method has been described for use in ruminants exposed to organophosphate insecticides. The method was used to measure cholinesterase activities in the plasma and erythrocytes of sheep, goats and cattle treated with organophosphate insecticides under field conditions. The animals were treated topically to control ectoparasites with malathion (0.05%) or diazinon (0.06%). The treated animals did not suffer from adverse effects related to cholinesterase inhibition. However, 2 h after treatments, plasma and erythrocyte cholinesterase activities significantly decreased in sheep by 20% and 39% and in goats by 18% and 30%, respectively, when compared with respective untreated control values. Diazinon did not significantly affect plasma and erythrocyte cholinesterase activities in the cattle. The results suggest that the described electrometric method could be efficiently used for detecting cholinesterase inhibition in ruminants exposed to organophosphate insecticides.
Mohammad
F K
FK
Department of Physiology, Division of Pharmacology and Toxicology, College of Veterinary Medicine, University of Mosul, PO Box 11136, Mosul, Iraq. fouadmohammad@yahoo.com
Alias
A S
AS
Faris
G A-M
GA
Al-Baggou
B Kh
BKh
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D002802:Cholinesterases / Q000097:blood / Q000187:drug effects / Q000378:metabolism*; D003976:Diazinon / Q000494:pharmacology; D004478:Ectoparasitic Infestations / Q000517:prevention & control / Q000662:veterinary; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D015511:Goat Diseases / Q000517:prevention & control; D006041:Goats; D007306:Insecticides / Q000494:pharmacology*; D008294:Malathion / Q000494:pharmacology; D008297:Male; D012756:Sheep; D012757:Sheep Diseases / Q000517:prevention & control; D013045:Species Specificity; D016896:Treatment Outcome
D002800:Cholinesterase Inhibitors; D007306:Insecticides; D002802:Cholinesterases; D008294:Malathion; D003976:Diazinon