[Interaction of the Laburnum anagyroides lectin with fucoantigens].
We studied interaction of the lectin from the bark of Golden Rain shrub (Laburnum anagyroides, LABA) with a number of basic fucose-containing carbohydrate antigens by changes in its tryptophan fluorescence. The strongest LABA binding was observed for the trisaccharide H of type 6 [alpha-L-Fucp-(1-2)-beta-D-Galp-(1-4)-D-Glc, Ka= 4.2 x 10(3) M(-1)]. The following antigens were bound with a weaker affinity: H-disaccharide alpha-L-Fucp-(1-2)-D-Gal, a glucoanalogue of tetrasaccharide Ley alpha-L-Fucp-(1-2)-beta-D-Galp-(1-4)-[alpha-L-Fucp-(1-3)]-D-Glc, and 6-fucosyl-N-acetylglucosamine, a fragment of core of the N-glycans family (Ka 1.1-1.7 x 10(3) M(-1)). The lowest binding was observed for L-fucose (Ka = 2.7 x 10(2) M-1) and trisaccharide Lea, (3-Galp-(1-3)-[a-L-Fucp-(1-4)]-GlcNAc (Ka = 6.4 x 10(2) M(-1)). The Lea, Lea, and Lex pentasaccharides and Leb hexasaccharide were not bound to LABA.
Piskarev
V E
VE
Bushueva
T L
TL
Iamskov
I A
IA
D000941:Antigens / Q000737:chemistry*; D005453:Fluorescence; D005643:Fucose / Q000737:chemistry*; D031296:Laburnum / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry* / Q000302:isolation & purification; D009844:Oligosaccharides / Q000737:chemistry; D014364:Tryptophan / Q000737:chemistry
D000941:Antigens; D037102:Lectins; D009844:Oligosaccharides; C027112:fucose-binding lectin; D005643:Fucose; D014364:Tryptophan