[Effect of static magnetic field on bone morphogenetic protein 2 and collagen type I of osteoblast cell].
OBJECTIVE
To study the effect of static magnetic field on osteoblast,and to explore the possibility of osteogenesis of static magnetic field.


METHODS
Rat calvarial osteoblasts were seeded on the 96-well culture plate, and exposed to 0.062T magnetic intensity field for 24 hours, 48 hours and 72 hours, respectively. Changes of BMP-2 and collagen type I in osteoblast were examined by Western blot. Immunohistochemical staining was carried out at different time periods. The data obtained were analyzed for ANOVA using SPSS 11.5 software package.


RESULTS
The results of Western blot showed that magnetic static field affected expression of BMP-2 and collagen type I in osteoblast. The expression of BMP-2 in 48-hour group increased 2.1 fold and the expression of collagen type I in 72-hour group increased 1.6 fold compared to the control group (P<0.01). Moreover, the staining color of the collagen type I in cell of magnetic treated group was deeper than that of the control.


CONCLUSIONS
Static magnetic field induced the expression of BMP-2 and stimulated secretion of collagen type I. The results indicate that osteoblasts are sensitive to 0.062T static magnetic field stimulation.
Qiu
Li-Hong
LH
College of Stomatology, Chinese Medical University, Shenyang 110002, Liaoning Province, China. drqlh@yahoo.com
Zhong
Ming
M
Tang
Xu-Na
XN
Wang
Zhao-Yuan
ZY
D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D002478:Cells, Cultured; D024042:Collagen Type I; D060526:Magnetic Fields; D010006:Osteoblasts; D051381:Rats; D013997:Time Factors
D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D024042:Collagen Type I