Inhibitory effects of various plant polyphenols on the toxicity of Staphylococcal alpha-toxin.
Staphylococcal alpha-toxin, known for its wide spectrum of biological activities, is involved in the pathogenesis of Staphylococcal infectious diseases. In recent years, various phytochemicals have been found to have antimicrobiological, including antibacterial, antiviral and antifungal, and antitoxic activities. We investigated whether several plant polyphenols inhibit alpha-toxin activities in vitro and in vivo. We found hop bract tannin (HBT) and apple condensed tannin (ACT) to exert inhibitory effects on alpha-toxin cytotoxicity. HBT also reduced the murine skin inflammatory effect and the lethality of alpha-toxin. These polyphenols formed aggregates with alpha-toxin and thereby inhibited its activities. Inhibition of alpha-toxin by HBT and ACT was dose dependent, suggesting that these polyphenols may be a useful adjunct to current treatments for alpha-toxin catalyzed Staphylococcal infectious diseases.
Choi
Oksun
O
Department of Molecular Infectiology, Graduate School of Medicine, Chiba University, Inohana 1-8-1, Chuo ward, Chiba 260-8670, Japan.
Yahiro
Kinnosuke
K
Morinaga
Naoko
N
Miyazaki
Masaru
M
Noda
Masatoshi
M
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001427:Bacterial Toxins / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D016466:CHO Cells; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003412:Cricetulus; D003876:Dermatitis, Atopic / Q000139:chemically induced / Q000517:prevention & control; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005419:Flavonoids / Q000494:pharmacology*; D018922:HL-60 Cells; D006460:Hemolysin Proteins / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D006801:Humans; D020014:K562 Cells; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010636:Phenols / Q000494:pharmacology*; D059808:Polyphenols; D013211:Staphylococcus aureus; D013634:Tannins / Q000494:pharmacology*; D014709:Vero Cells
D001427:Bacterial Toxins; D005419:Flavonoids; D006460:Hemolysin Proteins; D010636:Phenols; D059808:Polyphenols; D013634:Tannins; C021091:staphylococcal alpha-toxin