Transforming activity of purinergic receptor P2Y, G-protein coupled, 2 revealed by retroviral expression screening.
Colorectal cancer (CRC) is one of the leading causes of cancer death in humans. In order to identify novel cancer-promoting genes in CRC, we here constructed a retroviral cDNA expression library from a CRC cell line RKO, and used it for a focus formation assay with mouse 3T3 fibroblasts, leading to the identification of 42 independent cDNAs. One of such cDNAs turned out to encode purinergic receptor P2Y, G-protein coupled, 2 (P2RY2). The oncogenic potential of P2RY2 was confirmed in vitro with the focus formation assay as well as soft agar-growth assay, and also in vivo with a tumorigenicity assay in nude mice. While our P2RY2 cDNA encodes a protein with two amino-acid substitutions compared to the reported one, we have confirmed that the wild-type P2RY2 has a strong transforming potential as well. These results indicate an unexpected role of P2RY2 in the carcinogenesis of human cancers.
Hatanaka
Hisashi
H
Division of Functional Genomics, Jichi Medical University, Shimotsukeshi, Tochigi 329-0498, Japan.
Takada
Shuji
S
Choi
Young Lim
YL
Fujiwara
Shin-ichiro
S
Soda
Manabu
M
Enomoto
Munehiro
M
Kurashina
Kentaro
K
Watanabe
Hideki
H
Yamashita
Yoshihiro
Y
Sugano
Kentaro
K
Mano
Hiroyuki
H
D000818:Animals; D045744:Cell Line, Tumor; D002472:Cell Transformation, Viral; D003110:Colonic Neoplasms; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D018048:Receptors, Purinergic P2 / Q000235:genetics / Q000502:physiology*; D058473:Receptors, Purinergic P2Y2
C546385:P2RY2 protein, human; C546386:P2ry2 protein, mouse; D018048:Receptors, Purinergic P2; D058473:Receptors, Purinergic P2Y2