title: Trichostatin A induces myocardial differentiation of monkey ES cells.
abstract: Human embryonic stem (ES) cell lines are one of the possible sources of cardiac myocytes to be transplanted in patients with end-staged heart failure. However, prior to the application of human of ES cells for heart failure therapy, it is critical to validate their clinical use in large animals such as primates. Cynomolgus monkey ES cells have similar properties to human ES cells and can be used for primate studies. We demonstrate that 24-h stimulation by a histone deacetylase inhibitor, trichostatin A (TSA) facilitated myocardial differentiation of monkey ES cells with embryonic bodies that were seeded on gelatin-coated dishes. TSA-induced acetylating of histone-3/4 and expression of p300, one of the intrinsic histone acetyltransferases. Thus, such induction as well as inhibition of histone deacetylase may be involved in TSA-induced differentiation of cynomolgus monkey ES cells into cardiomyocytes.
authors: Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
authors: Mohsen
authors: M
authors: Hosseinkhani
authors: Koji
authors: K
authors: Hasegawa
authors: Koh
authors: K
authors: Ono
authors: Teruhisa
authors: T
authors: Kawamura
authors: Tomohide
authors: T
authors: Takaya
authors: Tatsuya
authors: T
authors: Morimoto
authors: Hiromichi
authors: H
authors: Wada
authors: Akira
authors: A
authors: Shimatsu
authors: Susana Gonzalez
authors: SG
authors: Prat
authors: Hirofumi
authors: H
authors: Suemori
authors: Norio
authors: N
authors: Nakatsuji
authors: Toru
authors: T
authors: Kita
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology / Q000187:drug effects*; D006877:Hydroxamic Acids / Q000494:pharmacology*; D008252:Macaca fascicularis; D009206:Myocardium / Q000166:cytology*
chemical_list: D006877:Hydroxamic Acids; C012589:trichostatin A