title: Behaviour of SH-SY5Y neuroblastoma cell line grown in different media and on different chemically modified substrates.
abstract: Among the parameters that can be tested in experiments on neuronal cell culture the use of different culture media and substrates represents a powerful assay to influence cell adhesion and differentiation. In this work, plasma-enhanced-chemical vapour depositions (PE-CVD) from acrylic acid and allylamine vapours have been performed to deposit coatings bearing oxygen (O)- and nitrogen (N)-containing functional groups on polyethylenetherephtalate (PET) surface. Human neuroblastoma SH-SY5Y cells were grown on plasma modified substrates and in presence of media containing different amount of fetal calf serum (FCS) or in serum-free medium containing cAMP. Our results showed that N-containing substrates improved cell adhesion, while the neurites sprouting was influenced by cell culture media. Interestingly, the presence of carboxylic groups on the modified surface can influence the expression of a differentiation marker, neurofilament-200 (NF-H), in cells grown in serum-containing media.
authors: Department of Pharmacology and Human Physiology, University of Bari, Piazza Giulio Cesare-Policlinico, 70124 Bari, Italy. m.buttiglione@fisiol.uniba.it
authors: M
authors: M
authors: Buttiglione
authors: F
authors: F
authors: Vitiello
authors: E
authors: E
authors: Sardella
authors: L
authors: L
authors: Petrone
authors: M
authors: M
authors: Nardulli
authors: P
authors: P
authors: Favia
authors: R
authors: R
authors: d'Agostino
authors: R
authors: R
authors: Gristina
mesh_terms: D000179:Acrylates / Q000737:chemistry; D000499:Allylamine / Q000737:chemistry; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002460:Cell Line; D020099:Coated Materials, Biocompatible; D003470:Culture Media / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D009447:Neuroblastoma; D016900:Neurofilament Proteins / Q000378:metabolism; D013499:Surface Properties; D014014:Tissue Adhesives / Q000737:chemistry
chemical_list: D000179:Acrylates; D020099:Coated Materials, Biocompatible; D003470:Culture Media; D016900:Neurofilament Proteins; D014014:Tissue Adhesives; C051774:neurofilament protein H; D000499:Allylamine; C036658:acrylic acid