Modelling the application of adaptive optics to wide-field microscope live imaging.
Wide-field fluorescence microscopy is an essential tool in modern cell biology. Unfortunately the image quality of fluorescence microscopes is often significantly degraded due to aberrations that occur under normal imaging conditions. In this article, we examine the use of adaptive optics technology to dynamically correct these problems to achieve close to ideal diffraction limited performance. Simultaneously, this technology also allows ultra-rapid focusing without having to move either the stage or the objective lens. We perform optical simulations to demonstrate the degree of correction that can be achieved.
Kam
Zvi
Z
Weizmann Institute of Science, Rehovot 76100, Israel. zvi.kam@weizmann.ac.il
Kner
Peter
P
Agard
David
D
Sedat
John W
JW