Simultaneous identification of natural dyes in the collection of drawings and maps from The Royal Chancellery Archives in Granada (Spain) by CE.
A simple and rapid capillary electrophoretic method with UV detection (CE-UV) has been developed for the identification of five natural dyes namely, carmine, indigo, saffron, gamboge and Rubia tinctoria root. The separation was performed in a fused-silica capillary of 64.5 cm length and 50 microm id. The running buffer was 40 mM sodium tetraborate buffer solution (pH 9.25). The applied potential was 30 kV, the temperature was 25 degrees C and detections were performed at 196, 232, 252, 300 and 356 nm. The injections were under pressure of 50 mbar during 13 s. The method was applied to the identification of carminic acid, gambogic acid, crocetin, indigotin, alizarin and purpurin in the collection of drawings and maps at the Royal Chancellery Archives in Granada (Spain). The method was validated by using HPLC as a reference method.
López-Montes
Ana
A
Department of Painting, Faculty of Fine Art, University of Granada, Granada, Spain.
Blanc García
Rosario
R
Espejo
Teresa
T
Huertas-Perez
José F
JF
Navalón
Alberto
A
Vílchez
José Luis
JL
D001154:Art / Q000266:history; D004396:Coloring Agents / Q000032:analysis* / Q000266:history; D019075:Electrophoresis, Capillary / Q000379:methods*; D049669:History, 16th Century; D049670:History, 17th Century; D049671:History, 18th Century; D049672:History, 19th Century; D008377:Maps as Topic; D013030:Spain; D014466:Ultraviolet Rays
D004396:Coloring Agents