title: Hybrid titania-aminosilane platforms evaluated with human mesenchymal stem cells.
abstract: The properties of hybrid aminopropyltriethoxysilane-tetraisopropylorthotitanate (APTS-TIPT) platforms prepared by a sol-gel route have been explored, and their biocompatibility was assayed after culture of human mesenchymal stem cells (hMSCs). The organic content of this material was observed to be preferably surface-oriented as indicated by microanalytical techniques. Furthermore, the surface showed characteristic amino-silane bands when explored by Raman spectroscopy as well as indications of silane and titanate condensation. Surface activity of the amino groups was probed by ultraviolet-visible spectroscopy imine derivatization and chemical force spectroscopy, showing a pH-dependent surface charge-induced potential. hMSCs cultured onto these surfaces showed relevant differences with respect to their behavior on gelatin-coated glass plates. Even if with a lower proliferative rate than controls, the cells develop long cytosolic prolongations in osteogenic differentiation medium, thus, supporting the idea of an APTS-TIPT stimulated process.
authors: Departamento de Física Aplicada C-XII-104, Universidad Autónoma de Madrid, 28049 Madrid, Spain. miguel.manso@uam.es
authors: M
authors: M
authors: Manso-Silván
authors: C
authors: C
authors: Rodríguez-Navas
authors: M
authors: M
authors: Arroyo-Hernández
authors: E
authors: E
authors: López-Elvira
authors: R
authors: R
authors: Gago
authors: L
authors: L
authors: Vázquez
authors: F
authors: F
authors: Agulló-Rueda
authors: A
authors: A
authors: Climent
authors: J M
authors: JM
authors: Martínez-Duart
authors: J P
authors: JP
authors: García-Ruiz
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism*; D002478:Cells, Cultured; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007097:Imines / Q000737:chemistry; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D012821:Silanes / Q000737:chemistry / Q000378:metabolism*; D014025:Titanium / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001672:Biocompatible Materials; D007097:Imines; D012821:Silanes; D014025:Titanium