Age-related changes in parvalbumin-positive interneurons in the striatum, but not in the sensorimotor cortex in dystonic brains of the dt(sz) mutant hamster.
In the dt(sz) hamster, a model of paroxysmal dystonia, an age-dependent increase in the activity of striatal projection neurons has been hypothesized to be based on a deficit of striatal parvalbumin-immunoreactive (PV(+)) interneurons at an age of most marked expression of dystonia (30-40 days of life). In the present study, the spontaneous age-dependent remission of paroxysmal dystonia in older dt(sz) hamsters (age>90 days) was found to coincide with a normalization of the density of striatal PV(+) interneurons. Furthermore, the arborization of these interneurons was lower in 31 day old dt(sz) hamsters, but was even higher in dt(sz) mutant at an age of >90 days than in control animals. Double-labeling with bromodeoxyuridine failed to show a retarded proliferation, while the number of interneurons with strong expression of PV mRNA was lower in young mutant hamsters. As shown by unaltered density of PV(+) interneurons in sensorimotor cortex of 31 day old dt(sz) hamsters, PV containing interneurons are not reduced throughout the whole brain at the sensitive age. The present data suggest that a retarded postnatal maturation of striatal PV(+) interneurons plays a critical role in paroxysmal dystonia.
Hamann
Melanie
M
Institute of Pharmacology and Toxicology, Department of Veterinary Medicine, Freie Universität Berlin (FU), Koserstr. 20, 14195 Berlin, Germany.
Richter
Angelika
A
Meillasson
Florence Vollenweider
FV
Nitsch
Cordula
C
Ebert
Ulrich
U
D000367:Age Factors; D000375:Aging; D000818:Animals; D030801:Animals, Genetically Modified; D003342:Corpus Striatum / Q000473:pathology*; D006224:Cricetinae; D004195:Disease Models, Animal; D004421:Dystonia / Q000235:genetics* / Q000473:pathology; D005260:Female; D017403:In Situ Hybridization / Q000379:methods; D007395:Interneurons / Q000378:metabolism*; D008297:Male; D010320:Parvalbumins / Q000235:genetics / Q000378:metabolism*; D012737:Sex Factors
D010320:Parvalbumins