Gingival crevicular fluid interleukin-1beta, prostaglandin E2 and periodontal status in a community population.
OBJECTIVE
Interleukin-1 beta (IL-1beta) and prostaglandin E(2) (PGE(2)) are key inflammatory mediators involved in periodontal disease. The purposes of this molecular cross-sectional epidemiological study were to investigate relationships in a community sample between mean concentrations of IL-1beta and PGE(2) in gingival crevicular fluid (GCF) and (1) clinical periodontal signs and (2) risk factors of host inflammatory response and/or periodontal disease.


METHODS
The sample comprised 6277 community-dwelling adults aged 52-74 years enrolled in the Atherosclerosis Risk in Communities (ARIC) study. IL-1beta and PGE(2) concentrations were measured using enzyme-linked immunosorbent assay. Person-level summary variables were computed for maximum pocket depth (MaxPD), maximum clinical attachment level (MaxCAL) and presence/absence of bleeding on probing (BOP). Mean GCF IL-1beta and PGE(2) concentrations were dependent variables in multiple linear regression models with periodontal measures and covariates as explanatory variables.


RESULTS
Both GCF IL-1beta and PGE(2) were positively related to MaxPD and BOP in multiple regression models (p<0.01). Increased levels of IL-1beta and PGE(2) were associated with body mass index >or=30 kg/m(2).


CONCLUSIONS
Higher levels of GCF IL-1beta and PGE(2) were significantly associated with clinical signs of periodontal disease and independently related to patient-based anthropomorphic measures, behaviours and exposures in community-dwelling adults.
Zhong
Y
Y
Department of Dental Ecology, School of Dentistry, University of North Carolina at Chapel Hill, NC 27599, USA.
Slade
G D
GD
Beck
J D
JD
Offenbacher
S
S
D000368:Aged; D015992:Body Mass Index; D003430:Cross-Sectional Studies; D015232:Dinoprostone / Q000032:analysis / Q000378:metabolism*; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006801:Humans; D053583:Interleukin-1beta / Q000032:analysis / Q000378:metabolism*; D016018:Least-Squares Analysis; D016014:Linear Models; D008297:Male; D008396:Maryland / Q000453:epidemiology; D008875:Middle Aged; D008910:Minnesota / Q000453:epidemiology; D008922:Mississippi / Q000453:epidemiology; D017720:Molecular Epidemiology; D009657:North Carolina / Q000453:epidemiology; D010510:Periodontal Diseases / Q000453:epidemiology* / Q000378:metabolism*; D010512:Periodontal Index; D012907:Smoking / Q000378:metabolism
D053583:Interleukin-1beta; D015232:Dinoprostone