Modification of Cepsilon mRNA expression by EBV-encoded latent membrane protein 1.
To evaluate the effect of expression of latent membrane protein (LMP) 1 encoded by Epstein-Barr virus (EBV) on Cepsilon mRNA expression, mRNA levels were examined by RT-PCR or Northern blot analysis upon transient transfection of LMP1 in the splenocytes derived from Brown-Norway rats with or without immunization with 2,4-dinitrophenyl-conjugated Ascaris suum antigen. Splenocytes were transfected with LMP1 expression vector, pSG5-LMP1, using lipofection method. Cepsilon mRNA levels were considerably increased by transfection with pSG5-LMP1 in the splenocytes derived from the nonimmunized rats; however, Cepsilon mRNA levels were decreased in the splenocytes derived from the immunized rats. Cepsilon mRNA expression in IgE-producing cells are modulated by LMP1, which might depend on the differentiation status of B cells upon exposure to allergen.
Hanashiro
Kazuhiko
K
1st Department of Physiology, School of Medicine, University of the Ryukyus, Nishihara, Okinawa, Japan.
Ohta
Shigeto
S
Sunagawa
Masanori
M
Nakamura
Mariko
M
Suzuki
Mikio
M
Kosugi
Tadayoshi
T
D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry / Q000276:immunology; D002478:Cells, Cultured; D003564:Cytidine Deaminase; D005786:Gene Expression Regulation; D006825:Hybridomas / Q000276:immunology / Q000473:pathology; D007139:Immunoglobulin epsilon-Chains / Q000235:genetics* / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011914:Rats, Inbred BN; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D014763:Viral Matrix Proteins / Q000235:genetics / Q000378:metabolism*
D000947:Antigens, Helminth; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D007139:Immunoglobulin epsilon-Chains; D012333:RNA, Messenger; D014763:Viral Matrix Proteins; C440639:ascaris suum 14-kDa antigen; D003564:Cytidine Deaminase