Synthesis and characterization of RGD-fatty acid amphiphilic micelles as targeted delivery carriers for anticancer agents.
Novel amphiphilic conjugates consisting of an Arg-Gly-Asp (RGD) peptide binding motif and aliphatic fatty acids of varying chain length (C10-C18) were synthesized and evaluated for their ability to form micelles and bind specifically to alphaVbeta3 integrin over-expressing tumor cells. The aphilphiles were characterized by IR, proton NMR and mass spectrometry. The size and zeta potential of the resultant micelles were ranged from 178 to 450 nm and - 13.5 to 39.6 mV, respectively. The critical micellar concentration (CMC), drug loading efficiency and tumor cell binding of these amphiphiles were determined. The CMC values, determined by pyrene fluorescent probe method, ranged from 0.02 to 0.12 mM for C14-RGD, C16-RGD and C18-RGD. The C18-RGD micelles with lowest CMC were found to increase the solubility of taxol, a model anticancer drug, by 87%. C18-RGD amphiphiles also exhibited significantly higher (12.1 +/- 1.14%, P < 0.05) binding to alphaVbeta3 integrin over-expressing human breast cancer cells (HTB-129) when compared to normal human epidermal keratinocyte (NHEK) cells (6.68 +/- 0.34). The results from this study demonstrated the feasibility of designing RGD-fatty acid amphiphiles as micellar drug delivery carriers to target to cancer cells.
Shen
Steve I
SI
Department of Pharmaceutics and Medicinal Chemistry, Thomas J. Long School of Pharmacy and Health Sciences, University of the Pacific, Stockton, CA 95211, USA.
Kotamraj
Phanidhara R
PR
Bhattacharya
Shiladitya
S
Li
Xiaoling
X
Jasti
Bhaskara R
BR
D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002626:Chemistry, Pharmaceutical; D004337:Drug Carriers / Q000138:chemical synthesis*; D016503:Drug Delivery Systems; D005227:Fatty Acids / Q000138:chemical synthesis* / Q000600:supply & distribution; D005260:Female; D005353:Fibronectins / Q000378:metabolism; D005456:Fluorescent Dyes; D006801:Humans; D039302:Integrin alphaVbeta3 / Q000235:genetics; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008823:Micelles; D009842:Oligopeptides / Q000138:chemical synthesis*; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000378:metabolism; D012995:Solubility; D013055:Spectrophotometry, Infrared
D000970:Antineoplastic Agents; D004337:Drug Carriers; D005227:Fatty Acids; D005353:Fibronectins; D005456:Fluorescent Dyes; D039302:Integrin alphaVbeta3; D008823:Micelles; D009842:Oligopeptides; D012710:Serum Albumin, Bovine; C047981:arginyl-glycyl-aspartic acid