title: Characterization of cytosolic glutathione S-transferases in striped bass (Morone saxitilis).
abstract: Electrophilic compounds are ubiquitous in the environment and aquatic life is inevitably affected. Glutathione S-transferases (GSTs) are a class of enzymes that facilitate the detoxification of these electrophiles in phase II metabolism. In this study, cytosolic GSTs were isolated and characterized from striped bass liver (Morone saxitilis). Nanospray liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to elucidate peptide sequences, and the proteins were found to have homology to rho and alpha by searching against the NCBI non-redundant database (nrDB). Catalytic activities of the cytosolic GSTs towards 1-chloro-2,4-dinitrobenzene (CDNB) were determined to be 141+/-34 and 155+/-65nmol/min/mg for males and females, respectively (both n=3). However, sex differences in classes expressed and activity toward CDNB were not statistically significant (p>0.05).
authors: Department of Environmental Toxicology, College of Agricultural and Environmental Sciences, University of California, 1 Shields Avenue, Davis, CA 95616-8588, USA.
authors: Sandra
authors: S
authors: Chang
authors: Rachel T
authors: RT
authors: Donham
authors: Andrea D
authors: AD
authors: Luna
authors: Dexter
authors: D
authors: Morin
authors: William T
authors: WT
authors: Jewell
authors: Ronald S
authors: RS
authors: Tjeerdema
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001492:Bass / Q000378:metabolism*; D003600:Cytosol / Q000201:enzymology*; D005786:Gene Expression Regulation; D005982:Glutathione Transferase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D005982:Glutathione Transferase