Reducing renal uptake of 111In-DOTATOC: a comparison among various basic amino acids.
OBJECTIVE
Several studies have reported significant renal toxicity after the use of a high dose of 90Y-DOTATOC. Thus, renal protection is necessary in treatments with 90Y-DOTA Tyr3-octreotide (DOTATOC). The infusion of certain positively charged amino acids has been shown to effectively reduce renal uptake of DOTATOC. In this study, we compared the effectiveness of three kinds of amino acids, D-lysine (lysine), L-arginine (arginine) and histidine, on renal protection in healthy rats and tried to determine which one was the most effective.


METHODS
Twenty SD healthy male rats were divided into 4 groups: lysine, histidine, arginine, and control. The rats were injected with a dose of 400 mg/kg of amino acid or 2 ml of phosphate-buffered saline (PBS) (as control) intraperitoneally. All rats were sacrificed at 4 hrs after the injection of 1 MBq 111In-DOTATOC. Samples of the kidney were taken and weighed carefully. The counts of radioactivity were measured by a gamma counter and renal concentrations were calculated and expressed as percent injected dose per gram (% ID/g).


RESULTS
The renal uptake of 111In-DOTATOC was significantly lower for all three kinds of amino acids when compared to the control group. The renal uptake of 111In-DOTATOC in the lysine group was significantly lower than those in the histidine and arginine groups. The renal uptake of 111In-DOTATOC in the histidine group was lower than that in the arginine group, but no statistical difference was noted.


CONCLUSIONS
Among these three amino acids, lysine had the best reduction rate of renal uptake of DOTATOC. Histidine was more effective than arginine but no statistical difference was noted.
Lin
Yung-Chang
YC
Department of Veterinary Medicine, National Chung-Hsing University, Taichung, Taiwan.
Hung
Guang-Uei
GU
Luo
Tsai-Yueh
TY
Tsai
Shih-Chuan
SC
Sun
Shung-Shung
SS
Hsia
Chien-Chung
CC
Chen
Shu-Ling
SL
Lin
Wan-Yu
WY
D024361:Amino Acids, Basic / Q000378:metabolism*; D000818:Animals; D007205:Indium Radioisotopes / Q000493:pharmacokinetics*; D007668:Kidney / Q000378:metabolism*; D008297:Male; D015282:Octreotide / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley
D024361:Amino Acids, Basic; D007205:Indium Radioisotopes; D015282:Octreotide; C106246:Edotreotide