title: Proteomic profile of mouse fibroblasts with a targeted disruption of the peroxisome proliferator activated receptor-beta/delta gene.
abstract: The peroxisome proliferator activated receptor-beta (PPARbeta) plays an essential role in lipid metabolism, immune modulation, differentiation and cell proliferation. There is also strong evidence for a function in oncogenesis and tumor vascularization, but the underlying molecular mechanisms remain elusive. In the present study, we have used fibroblasts derived from Pparb wild-type and null mice to determine by 2-DE and PMF analysis the contribution of PPARbeta to the protein profile of fibroblasts. Thirty-one differentially expressed proteins of different functional categories were identified. For at least two proteins a role in tumorigenesis and/or tumor vascularization has previously been reported: while the calcium intracellular channel-4 (CLIC4) was expressed at lower levels in Pparb null cells, expression of the cellular retinol binding protein 1 (CRBP1) was enhanced. Clic4 and Crbp1 gene expression patterns observed in different experimental settings in vitro and in vivo confirmed the proteomics data. Our findings indicate that the expression of a defined set of proteins is altered in fibroblasts and endothelial cells from Pparb null mice, that this is due to aberrant gene regulation, and that the altered expression of these proteins is consistent with the tumor vascularization phenotype of Pparb null mice.
authors: Institute of Molecular Biology and Tumor Research, Philipps University, Marburg, Germany.
authors: Jürgen
authors: J
authors: Adamkiewicz
authors: Kerstin
authors: K
authors: Kaddatz
authors: Markus
authors: M
authors: Rieck
authors: Bernhard
authors: B
authors: Wilke
authors: Sabine
authors: S
authors: Müller-Brüsselbach
authors: Rolf
authors: R
authors: Müller
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018118:Chloride Channels / Q000235:genetics / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D005347:Fibroblasts / Q000737:chemistry*; D018390:Gene Targeting; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D047494:PPAR delta / Q000235:genetics* / Q000378:metabolism; D048068:PPAR-beta / Q000235:genetics* / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D012177:Retinol-Binding Proteins / Q000235:genetics / Q000378:metabolism; D054840:Retinol-Binding Proteins, Cellular
chemical_list: D018118:Chloride Channels; D047494:PPAR delta; D048068:PPAR-beta; D020543:Proteome; C518456:RBP1 protein, human; D012177:Retinol-Binding Proteins; D054840:Retinol-Binding Proteins, Cellular