title: Hydrogen peroxide in exhaled breath condensate (EBC) vs eosinophil count in induced sputum (IS) in parenchymal vs airways lung diseases.
abstract: We compared exhaled breath condensate (EBC) and induced sputum (IS) for assessing inflammation in pulmonary diseases in patients with obstructive lung disease (n = 20), persistent cough >6 months (n = 20), interstitial lung disease (n = 25) and controls (n = 10). EBC was collected by suspending a Teflon perfluoroalkoxy tube installed in an ice-filled container and connected to a polypropylene test tube. IS was recovered after 20' inhalation of 3% saline with an ultrasonic nebulizer, and 300 cells were differentially counted in cytospin Giemsa-stained slides. H(2)0(2) was measured by a method based on oxidation of phenolsulfonphthalein (phenol red) mediated by horseradish peroxidases and H(2)0(2). Pulmonary function tests were performed by conventional methods. H(2)0(2) levels in EBC and % eosinophils in IS were significantly different between groups. A positive and significant correlation was found between % eosinophils in IS and the levels of H(2)0(2) in EBC for each group and for all patients combined.
authors: Institute of Pulmonary and Allergic Diseases, Tel-Aviv Sourasky Medical Center and National Laboratory Service for ILD, Sakler Faculty of Medicine, Tel-Aviv University, 6 Weizman Street, Tel-Aviv 64239, Israel.
authors: Elizabeth
authors: E
authors: Fireman
authors: Moshe
authors: M
authors: Shtark
authors: Israel E
authors: IE
authors: Priel
authors: Robert
authors: R
authors: Shiner
authors: Ram
authors: R
authors: Mor
authors: Shmuel
authors: S
authors: Kivity
authors: Zvi
authors: Z
authors: Fireman
mesh_terms: D000328:Adult; D000368:Aged; D001249:Asthma / Q000097:blood / Q000175:diagnosis* / Q000503:physiopathology; D015415:Biomarkers / Q000032:analysis; D001944:Breath Tests / Q000379:methods*; D003371:Cough / Q000097:blood / Q000175:diagnosis* / Q000503:physiopathology; D004804:Eosinophils; D045853:Exhalation; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis*; D007958:Leukocyte Count; D017563:Lung Diseases, Interstitial / Q000097:blood / Q000175:diagnosis* / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood / Q000175:diagnosis* / Q000503:physiopathology; D012129:Respiratory Function Tests; D012720:Severity of Illness Index; D013183:Sputum / Q000166:cytology*
chemical_list: D015415:Biomarkers; D006861:Hydrogen Peroxide