title: The maternally localized RNA fatvg is required for cortical rotation and germ cell formation.
abstract: Fatvg is a localized maternal transcript that translocates to the vegetal cortex of Xenopus laevis oocytes through both the METRO and Late RNA localization pathways. It is a member of a gene family that functions in vesicular trafficking. Depletion of the maternal store of fatvg mRNA results in a dual phenotype in which embryos are ventralized and also lack primordial germ cells. This complex fatvg loss of function phenotype is the result of stabilization of the dorsalizing factor beta-catenin at the vegetal pole and the inability of the germ cell determinants to move to their proper locations. This is coincident with the inhibition of cortical rotation and the abnormal aggregation of the germ plasm. Fatvg protein is located at the periphery of vesicles in the oocyte and embryo, supporting its proposed role in vesicular trafficking in the embryo. These results point to a common fundamental mechanism that is regulated by fatvg through which germ cell determinants and dorsalizing factors segregate during early development.
authors: Department of Molecular Genetics, University of Texas, M.D. Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX 77030, USA.
authors: Agnes P
authors: AP
authors: Chan
authors: Malgorzata
authors: M
authors: Kloc
authors: Carolyn A
authors: CA
authors: Larabell
authors: Mark
authors: M
authors: LeGros
authors: Laurence D
authors: LD
authors: Etkin
mesh_terms: D000818:Animals; D001483:Base Sequence; D016764:Cell Polarity; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D005260:Female; D066298:In Vitro Techniques; D008565:Membrane Proteins / Q000235:genetics*; D020319:Oligodeoxyribonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D015388:Organelles / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*; D029867:Xenopus Proteins / Q000235:genetics* / Q000378:metabolism; D014982:Xenopus laevis; D051176:beta Catenin / Q000378:metabolism
chemical_list: C495266:CTNNB1 protein, Xenopus; D008565:Membrane Proteins; D020319:Oligodeoxyribonucleotides, Antisense; C401544:PLIN2 protein, Xenopus; D012333:RNA, Messenger; D029867:Xenopus Proteins; D051176:beta Catenin