Corosolic acid isolation from the leaves of Eriobotrta japonica showing the effects on carbohydrate metabolism and differentiation of 3T3-L1 adipocytes.
The extracts of Eriobotrta japonica leaves with the 3H-glucose uptaking activity in 3T3-L1 adipocytes were separated by TLC for two times. On the basis of UV-vis spectral, NMR and MS data, corosolic acid was identified as activity components. Moreover, the effects of corosolic acid on carbohydrate metabolism and differentiation of 3T3-L1 adipocytes was studied. The results showed that 3H-glucose uptaking rate in different concentrations of corosolic acid (15 micromol/L, 30 micromol/L, and 45 micromol/L) group were increased to 108.1%, 112.2% ,118.6%, respectively, compared to control group (without corosolic acid) (p<0.01). Corosolic acid suppressed the differentiation of 3T3-L1 pre-adipocytes and down-regulated the expression of PPAR-gamma and C/EBP-alpha mRNA (p<0.01, vs control group). Corosolic acid promotes the 3H-glucose uptaking, suppresses the differentiation and downregulates the expression of PPAR-gamma and C/EBP-alpha mRNA in 3T3-L1 adipocytes.
Zong
Wei
W
School of Food and Biological Engineering, Zhengzhou University of Light Industry, 5 Dongfeng Road, Zhengzhou, Henan, China 450002. zongwei1965@126.com
Zhao
Guangyuan
G
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D050260:Carbohydrate Metabolism / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D031983:Eriobotrya / Q000737:chemistry*; D015870:Gene Expression / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D051379:Mice; D047495:PPAR gamma / Q000378:metabolism; D010936:Plant Extracts; D018515:Plant Leaves / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*
D004365:Drugs, Chinese Herbal; D047495:PPAR gamma; D010936:Plant Extracts; D012333:RNA, Messenger; D014315:Triterpenes; C113861:corosolic acid; D005947:Glucose