Simultaneous analysis of cytokines and co-stimulatory molecules concentrations by ELISA technique and of probabilities of measurable concentrations of interleukins IL-2, IL-4, IL-5, IL-6, CXCL8 (IL-8), IL-10, IL-13 occurring in plasma of healthy blood donors.
BACKGROUND
The cytokine network remarkably influences homeostasis. There is not a complex analysis describing physiological values of soluble cytokines in healthy individuals.


METHODS
Authors used the ELISA method to detect soluble cytokines and costimulatory molecules simultaneously in the plasma of 30 blood donors.


RESULTS
Data are shown as (a range of concentrations; median) IL-2 (0-74.48 pg/mL; 0), IL-4 (0-65.18 pg/mL; 0), IL-5 (0-86.69 pg/mL; 0), IL-6 (0-73.17 pg/mL; 0), IL-8 (0-74.8 pg/mL; 0), IL-10 (0-12.6 pg/mL; 0), IL-13 (0-3.9 pg/mL; 0), sCD40L (0-3.5 ng/mL; 0), and TNF-alpha (0-67.6; 0). For the IL-6 receptor, the concentration was 75.89 +/- 35.83 ng/mL (average +/- SD), sCD23 28.9 +/- 15.22 ng/mL, and TGFbeta1 14.98 +/- 12.39 ng/mL. A special approach was taken for the analysis of values, which had median 0. A model for the probability of detectable level's occurrence for each interleukin was formulated with intervals of confidence.


CONCLUSIONS
These results provide reference values for cytokine levels in the plasma of healthy individuals.
Martin
Klabusay
K
Department of Internal Medicine-Hematooncology, University Hospital Brno, Czech Republic.
Viera
Kohutova
K
Petr
Coupek
C
Marie
Nenickova
N
Eva
Tesarova
T
D000328:Adult; D001774:Blood Chemical Analysis / Q000379:methods*; D001782:Blood Donors; D023201:CD40 Ligand / Q000097:blood; D016207:Cytokines / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000097:blood; D018793:Interleukin-13 / Q000097:blood; D007376:Interleukin-2 / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D015848:Interleukin-5 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D016209:Interleukin-8 / Q000097:blood; D008297:Male; D017455:Receptors, IgE / Q000097:blood; D019947:Receptors, Interleukin-6 / Q000097:blood; D012016:Reference Values; D053773:Transforming Growth Factor beta1 / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D016207:Cytokines; C508609:IL10 protein, human; C508598:IL4 protein, human; C508599:IL5 protein, human; C508600:IL6 protein, human; D018793:Interleukin-13; D007376:Interleukin-2; D015848:Interleukin-5; D015850:Interleukin-6; D016209:Interleukin-8; D017455:Receptors, IgE; D019947:Receptors, Interleukin-6; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D023201:CD40 Ligand; D015847:Interleukin-4