Determination of synthetic acaricides residues in beeswax by high-performance liquid chromatography with photodiode array detector.
A multiresidue HPLC method for identification and quantification of the synthetic acaricides fluvalinate, coumaphos, bromopropylate and its metabolite 4,4'-dibromobenzophenone in beeswax has been developed. Different techniques were tested and modified. The method consists of a sample preparation with isooctane followed by solid phase extraction using Florisil columns. Determination of the synthetic acaricides is achieved by HPLC with a photodiode array detector. Analytical performance of the proposed method, including sensitivity, accuracy and precision was satisfactory. The LOD for the analytes varied between 0.1 and 0.2 microg g(-1) wax and the recoveries between 70 and 110%. Relative standard deviation of the repeatability of the method is <15% and reproducibility is <31%.
Adamczyk
Sabine
S
Department of Animal Production and Food Science, Veterinary Faculty, University of Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain.
Lázaro
Regina
R
Pérez-Arquillué
Consuelo
C
Herrera
Antonio
A
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003372:Coumaphos / Q000032:analysis; D009570:Nitriles / Q000032:analysis; D010573:Pesticide Residues / Q000032:analysis*; D011722:Pyrethrins / Q000032:analysis; D014885:Waxes / Q000737:chemistry*
D009570:Nitriles; D010573:Pesticide Residues; D011722:Pyrethrins; D014885:Waxes; C038228:beeswax; C034227:fluvalinate; D003372:Coumaphos