title: Lipopolythioureas: a new non-cationic system for gene transfer.
abstract: A DNA-transfection protocol has been developed that makes use of thiourea non-cationic synthetic lipid, N-[1,3-bis(carbamothioylamino)propan-2-yl]-2-(dialkycarbamoylmethoxy)acetamide. It was found that these new compounds could be formulated without helper lipid and that the N-decanoyl and N-lauryl derivatives transfected B16 cells in the presence of serum with an efficiency at the same level as cationic lipids, under identical conditions. In vivo transfection using intratumoral injection was also investigated. It was found that compounds 18c and 19 showed an efficiency of the same magnitude as naked DNA and cationic lipid.
authors: Inserm, U640, Paris, F-75006, CNRS, UMR8151, Paris, F-75006, Ecole Nationale Supérieure de Chimie de Paris, Paris, France.
authors: Jeanne
authors: J
authors: Leblond
authors: Nathalie
authors: N
authors: Mignet
authors: Céline
authors: C
authors: Largeau
authors: Maria-Vittoria
authors: MV
authors: Spanedda
authors: Johanne
authors: J
authors: Seguin
authors: Daniel
authors: D
authors: Scherman
authors: Jean
authors: J
authors: Herscovici
mesh_terms: D000818:Animals; D002412:Cations; D004247:DNA / Q000008:administration & dosage* / Q000737:chemistry; D018014:Gene Transfer Techniques; D008055:Lipids / Q000138:chemical synthesis / Q000737:chemistry*; D008081:Liposomes; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008546:Melanoma, Experimental / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D010957:Plasmids / Q000008:administration & dosage; D013890:Thiourea / Q000138:chemical synthesis / Q000737:chemistry*; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D002412:Cations; D008055:Lipids; D008081:Liposomes; D004247:DNA; D008156:Luciferases; D013890:Thiourea