Non-fouling microfluidic chip produced by radio frequency tetraglyme plasma deposition.
This Technical Note presents the direct surface modification of a glass/PTFE hybrid microfluidic chip, via radio frequency glow discharge plasma polymerisation of tetraethlylene glycol dimethylether (tetraglyme), to produce hydrophilic, non-fouling, PEO-like surfaces. We use several techniques including X-ray photoelectron spectroscopy (XPS), direct enzyme-linked immunosorbent assays (ELISA) and immunofluorescent imaging to investigate the channel coatings. Our results indicate the successful deposition of a PEO-like coating onto microchannel surfaces that has both solution and shelf stability (>3 months) and is capable of preventing fibrinogen adsorption to the microchannel surfaces.
Salim
Malinda
M
Department of Chemical and Process Engineering, University of Sheffield, Mappin Street, Sheffield, UKS1 3JD. p.c.wright@sheffield.ac.uk.
Mishra
Gautam
G
Fowler
Gregory J S
GJ
O'sullivan
Brian
B
Wright
Phillip C
PC
McArthur
Sally L
SL
D000327:Adsorption; D004797:Enzyme-Linked Immunosorbent Assay; D004798:Enzymes / Q000737:chemistry; D005026:Ethylene Glycols / Q000737:chemistry*; D005340:Fibrinogen / Q000737:chemistry; D006801:Humans; D046210:Microfluidic Analytical Techniques; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D008856:Microscopy, Fluorescence; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011846:Radio Waves; D013052:Spectrometry, X-Ray Emission; D013499:Surface Properties
D004798:Enzymes; D005026:Ethylene Glycols; D011108:Polymers; D011092:Polyethylene Glycols; C441631:tetraglyme; D005340:Fibrinogen