title: Multilocus sequence analysis of atypical Borrelia burgdorferi sensu lato isolates--description of Borrelia californiensis sp. nov., and genomospecies 1 and 2.
abstract: Taxonomy of Borrelia burgdorferi sensu lato (s.l.) was recently improved by the use of multilocus sequence analysis (MLSA), a new approach to replace the cumbersome DNA-DNA hybridization method [Richter et al., 2006. Int. J. Syst. Evol. Microbiol. 156, 873-881]. In this study, we used this methodology to classify B. burgdorferi s.l. strains isolated both in Europe and the United States, the exact taxonomic status of which remained unclear. We conclude that MLSA can surpass the discrimination power of whole DNA-DNA hybridization, and we delineate three new North American B. burgdorferi s.l. species. In contrast, European atypical strains constituted a subgroup of B. burgdorferi sensu stricto (s.s.).
authors: Laboratoire des Spirochètes, Institut Pasteur, 28 rue du Dr. Roux, F-75724 Paris Cedex 15, France. dpostic@pasteur.fr
authors: Danièle
authors: D
authors: Postic
authors: Martine
authors: M
authors: Garnier
authors: Guy
authors: G
authors: Baranton
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D015748:Borrelia burgdorferi Group / Q000145:classification* / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis; D004463:Ecology; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D004269:DNA, Bacterial