title: Biochemical and molecular characterization of the homocysteine S-methyltransferase from broccoli (Brassica oleracea var. italica).
abstract: Plants are known for their unique ability to synthesize methionine from S-methylmethionine (SMM) and homocysteine using the enzyme SMM: homocysteine S-methyltransferase (HMT) in the SMM cycle. Two cDNAs exhibiting HMT activity were cloned from broccoli and functionally expressed in E. coli. One cDNA, that encodes an enzyme with high substrate specificity for homocysteine, was designated as BoHMT1. The other cDNA was the BoSMT gene that we previously characterized and encodes a selenocysteine methyltransferase (Lyi, S.M., Heller, L.I., Rutzke, M., Welch, R.M., Kochian, L.V., Li, L., 2005. Molecular and biochemical characterization of the selenocysteine Se-methyltransferase gene and Se-methylselenocysteine synthesis in broccoli. Plant Physiol. 138, 409-420). Both exist as single gene sequences in the broccoli genome. While BoSMT expression was extremely low or undetectable in broccoli plants unless the plants were exposed to selenium, the BoHMT1 mRNA accumulated in most tissues of the plant except older leaves. In contrast to BoSMT whose expression was dramatically upregulated by treating plants with selenate, the transcript levels of BoHMT1 were not markedly affected in plants exposed to selenium. BoHMT1 expression responded significantly to changes in plant sulfur status. However, its expression was not dramatically affected in plants treated with methionine, SMM, homocysteine, or the heavy metal, cadmium. The differences in the substrate specificity and gene expression in response to changes in plant sulfur and selenium status between BoHMT1 and BoSMT suggest that the enzymes encoded by these two genes play distinct roles in sulfur and selenium metabolism in broccoli.
authors: USDA-ARS, Plant, Soil and Nutrition Laboratory, Cornell University, Ithaca, NY 14853, USA.
authors: Sangbom M
authors: SM
authors: Lyi
authors: Xin
authors: X
authors: Zhou
authors: Leon V
authors: LV
authors: Kochian
authors: Li
authors: L
authors: Li
mesh_terms: D000595:Amino Acid Sequence; D001937:Brassica / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D002104:Cadmium / Q000494:pharmacology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D050904:Homocysteine S-Methyltransferase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008715:Methionine / Q000494:pharmacology; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D012643:Selenium / Q000494:pharmacology; D016415:Sequence Alignment; D013379:Substrate Specificity; D013431:Sulfates / Q000494:pharmacology; D013455:Sulfur / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D012333:RNA, Messenger; D013431:Sulfates; D002104:Cadmium; D013455:Sulfur; D008715:Methionine; D050904:Homocysteine S-Methyltransferase; D012643:Selenium