Thermosensitivity of the Saccharomyces cerevisiae gpp1gpp2 double deletion strain can be reduced by overexpression of genes involved in cell wall maintenance.
A Saccharomyces cerevisiae strain in which the GPP1 and GPP2 genes, both encoding glycerol-3-phosphate phosphatase isoforms, are deleted, displays both osmo- and thermosensitive (ts) phenotypes. We isolated genes involved in cell wall maintenance as multicopy suppressors of the gpp1gpp2 ts phenotype. We found that the gpp1gpp2 strain is hypersensitive to cell wall stress such as treatment with beta-1,3-glucanase containing cocktail Zymolyase and chitin-binding dye Calcofluor-white (CFW). Sensitivity to Zymolyase was rescued by overexpression of SSD1, while CFW sensitivity was rescued by SSD1, FLO8 and WSC3-genes isolated as multicopy suppressors of the gpp1gpp2 ts phenotype. Some of the isolated suppressor genes (SSD1, FLO8) also rescued the lytic phenotype of slt2 deletion strain. Additionally, the sensitivity to CFW was reduced when the cells were supplied with glycerol. Both growth on glycerol-based medium and overexpression of SSD1, FLO8 or WSC3 had additive suppressing effect on CFW sensitivity of the gpp1gpp2 mutant strain. We also confirmed that the internal glycerol level changed in cells exposed to cell wall perturbation.
Wojda
Iwona
I
Department of Invertebrate Immunology, Institute of Biology, Maria Curie-Sklodowska University, Akademicka 19, 20-033, Lublin, Poland. wojda@hektor.umcs.lublin.pl
Bebelman
Jan-Paul
JP
Jakubowicz
Teresa
T
Siderius
Marco
M
D002473:Cell Wall / Q000201:enzymology / Q000502:physiology*; D017353:Gene Deletion; D015870:Gene Expression; D005990:Glycerol / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics; D010641:Phenotype; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D013696:Temperature
D007527:Isoenzymes; D029701:Saccharomyces cerevisiae Proteins; C055686:glyceraldehyde 3-phosphate phosphatase; D010744:Phosphoric Monoester Hydrolases; D005990:Glycerol