title: Comparative study between ELISA, immuno-diffusion and cell bound immuno assay techniques for detection of anti-bovine viral diarrhea antibodies in calves of some farms in alexandria and behira governorates.
abstract: Rapid, highly sensitive and specific technique is essential need for early diagnosis of Bovine Viral Diarrhea (BVD) infection in calves to prevent, control and eradicate the persistently infected animals; so, ELISA, Immuno-diffusion (ID) and Cell bound immuno assay (CBIA) techniques were compared for detection of anti-Bovine Viral Diarrhea (anti-BVD) antibodies in 240 calves blood samples of some farms in Alexandria and Behira Governorates (120 each). Out of total 240 tested blood samples; 143 (59.6 %), 47 (19.6 %), and 155 (64.6 %) were positive for anti-BVD antibodies using ELISA, ID and CBIA respectively. The highest detection rate was found in diseased calves at Behira Governorate (83.5 %) using CBIA technique, while the lowest detection rate was found in contact calves at Alexandria Governorate (3.2 %) using ID technique. The percentage of agreement between CBIA and each of ELISA and ID was 95% and 55% respectively for detection of anti-BVD antibodies in Calve sera. The percentage of sensitivity between CBIA and each of ELISA and ID was 100 %, while the percentage of specificity between CBIA and each of ELISA and ID was 87.6 % and only 44 % respectively for detection of anti-BVD antibodies in calves sera. This study showed that, the CBIA as well as ELISA techniques were highly sensitive and specific tests for detection of anti-BVD antibodies in calves sera when compared to ID technique. Moreover, CBIA has many advantages over ELISA technique; Firstly, CBIA is rapid, simple, cheap and not need to ELISA reader as in ELISA technique. Secondarily; the microtitre plate containing BVD infected tissue culture monolayer can be preserved for long time in comparison to prepared ELISA plates.
authors: Microbiology Department, Animal Health Research Institute, Dokki, Alexandria Branch, Alexandria, Egypt. Bahgat27@hotmail.com.
authors: Bahgat Z
authors: BZ
authors: Youssef