Controlled release of platelet growth factors enhances bone regeneration at rabbit calvaria.
OBJECTIVE
Platelet-rich plasma (PRP) has been clinically employed to promote bone regeneration. However, few studies have investigated the enhancement of biological function of platelet growth factors after integration of PRP into biomaterials. In this study, the feasibility of gelatin hydrogels for controlled release of platelet growth factors and the consequent enhancement of PRP-induced bone regeneration were evaluated in rabbit calvarial defect.


METHODS
Gelatin hydrogels incorporating PRP, PRP-activated thrombin, or an empty gelatin hydrogel were applied to the defect, or the defect was left untreated. Bone regeneration was evaluated by microfocus computed tomography, peripheral quantitative computed tomography, and histological examinations.


RESULTS
Successful bone regeneration was observed at the bone defect applied with the gelatin hydrogel incorporating PRP, which is in marked contrast to other groups.


CONCLUSIONS
The gelatin hydrogel is a promising material capable of controlled release of platelet growth factors to enhance bone regeneration.
Hokugo
Akishige
A
Department of Biomaterials, Field of Tissue Engineering, Institute for Frontier Medical Sciences, Kyoto University, Kawara-cho Shogoin, Sakyo-ku, Kyoto, Japan.
Sawada
Yasunori
Y
Hokugo
Rie
R
Iwamura
Hiromitsu
H
Kobuchi
Masakiyo
M
Kambara
Toshiyuki
T
Morita
Shosuke
S
Tabata
Yasuhiko
Y
D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use*; D001861:Bone Regeneration / Q000187:drug effects*; D003692:Delayed-Action Preparations; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000627:therapeutic use*; D036341:Intercellular Signaling Peptides and Proteins / Q000008:administration & dosage*; D053657:Platelet-Rich Plasma; D011817:Rabbits; D012886:Skull / Q000601:surgery; D014057:Tomography, X-Ray Computed
D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D036341:Intercellular Signaling Peptides and Proteins; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate