Evaluation of ketoprofen formulations via penetration rate and irritation in vivo study.
The purpose of the present study was to design an optimal ketoprofen gel with appropriate penetration rate, shortened lag time and acceptable skin irritation. The combination of different mechanism enhancers including nonivamide, menthol and ethanol were used as multi-enhancers for producing a synergistic enhancement effect and reducing the skin irritation via diminishing the used amount of enhancers. The central composite design was applied to prepare a systemic formulation. The penetration rate (PR), lag time (LT) and skin irritation score (TIS) of a commercial product (Formax plus gel containing 3% ketoprofen) were determined by in vivo study and used as a criterion for designed formulations. The PR, LT and TIS of commercial product were 462.2+/-162.5 microg/h, 0.6+/-0.1 h and 12.7+/-0.6, respectively. Among these designed experimental formulations, four formulations including F07 (code: -1/+1/-1), F11 (code: +1/+1/-1), F13 (code: 0/0/-1.732) and F14 (code: 0/+1.732/0), their PR was not smaller and LT and TIS were not greater than that of commercial product, indicating that these experimental ketoprofen gels could be used in the clinical situation.
Huang
Yaw-Bin
YB
Graduate Institute of Clinical Pharmacy, College of Pharmacy, Kaohsiung Medical University, 100 Shih-Chuan 1st Road, Kaohsiung City 80708, Taiwan, ROC.
Wang
Ren-Jiunn
RJ
Chang
Jui-Sheng
JS
Tsai
Yi-Hung
YH
Wu
Pao-Chu
PC
D000818:Animals; D002626:Chemistry, Pharmaceutical; D005782:Gels; D007660:Ketoprofen / Q000737:chemistry* / Q000493:pharmacokinetics; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D023422:Skin Irritancy Tests
D005782:Gels; D007660:Ketoprofen