title: Nucleotide sequence of the S and M RNA segments of a Groundnut bud necrosis virus isolate from Vigna radiata in India.
abstract: Nucleotide sequence of the S and M RNA segments of a Groundnut bud necrosis virus isolate from Vigna radiata (mungbean isolate, GBNV-MB) was determined and compared with another isolate from Arachis hypogaea (groundnut isolate, GBNV-type). Comparative sequence analysis revealed that the genome organization of the S and M RNA segments of both GBNV-MB and GBNV-type isolates was similar. However, considerable differences were observed in their intergenic regions (IGRs) and the glycoprotein precursors (Gn/Gc) of the M RNA segments. M RNA IGRs of GBNV-MB and GBNV-type isolates differed in size by 14 nucleotides. This difference was of 75 nucleotides in another GBNV isolate from Lycopersicon esculentum (tomato). Sequence comparison of the M RNA IGRs of GBNV isolates from mungbean, groundnut and tomato from India revealed 56-89% sequence identity. The topology of Gn/Gc revealed the presence of both N-glycosylation (5) and O-glycosylation (1) sites in GBNV-MB isolate, whereas only N-glycosylation sites (4) were present in GBNV-type isolate.
authors: Unit of Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, India.
authors: R K
authors: RK
authors: Saritha
authors: R K
authors: RK
authors: Jain
mesh_terms: D010367:Arachis / Q000821:virology; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D021901:DNA, Intergenic; D004279:DNA, Viral / Q000235:genetics; D007887:Fabaceae / Q000821:virology*; D006023:Glycoproteins / Q000235:genetics; D007194:India; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000139:chemically induced; D011498:Protein Precursors / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics*; D013045:Species Specificity; D017865:Tospovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D014764:Viral Proteins / Q000235:genetics
chemical_list: D017931:DNA Primers; D021901:DNA, Intergenic; D004279:DNA, Viral; D006023:Glycoproteins; D011498:Protein Precursors; D012367:RNA, Viral; D014764:Viral Proteins