HPLC quantification of seven quaternary benzo[c]phenanthridine alkaloids in six species of the family Papaveraceae.
The content of the seven quaternary benzo[c]phenanthridine alkaloids (QBA) sanguinarine (SA), chelerythrine (CHE), chelirubine (CHR), chelilutine (CHL), sanguilutine (SL), sanguirubine (SR) and macarpine (MA) was determined in the underground part of six plant species of the family Papaveraceae (Sanguinaria canadensis L., Dicranostigma lactucoides HOOK.f.et THOMS, Chelidonium majus L., Macleaya cordata (Willd.), Macleaya microcarpa (Maxim) and Stylophorum lasiocarpum (Oliv.)). HPLC method with reversed phase column Synergi Max-RP C-12 Phenomenex was used, mobile phase consisted of heptanesulfonic acid (0.01 mol/l) with triethanolamine (0.1 mol/l) in redistilled water, pH 2.5, acetonitrile gradient 25-60% during 25 min. Detection was performed at 280 nm. The highest content of SA and CHE was found in the roots of D. lactucoides (1.99%, resp. 3.43% of the dried roots). In rhizomes of S. canadensis was their content more then two times lower.
Suchomelová
Jana
J
Department of Biochemistry, Faculty of Medicine, Masaryk University, Komenského nám. 2, CZ - 662 43 Brno, Czech Republic.
Bochoráková
Hana
H
Paulová
Hana
H
Musil
Pavel
P
Táborská
Eva
E
D000097:Acetonitriles / Q000737:chemistry; D000470:Alkaloids / Q000032:analysis* / Q000737:chemistry; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004983:Ethanolamines / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D000432:Methanol / Q000737:chemistry; D015394:Molecular Structure; D028101:Papaveraceae / Q000033:anatomy & histology / Q000737:chemistry*; D010617:Phenanthridines / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000033:anatomy & histology / Q000737:chemistry*; D012044:Regression Analysis; D015203:Reproducibility of Results; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet; D014867:Water / Q000737:chemistry
D000097:Acetonitriles; D000470:Alkaloids; D004983:Ethanolamines; D010617:Phenanthridines; D010936:Plant Extracts; D014867:Water; C009546:triethanolamine; D000432:Methanol; C032159:acetonitrile