title: Role of gap junctions in regulation of progesterone secretion by ovine luteal cells in vitro.
abstract: To evaluate the role of gap junctions in the regulation of progesterone secretion, two experiments were conducted. In Experiment 1, luteal cells obtained on days 5, 10, and 15 were cultured overnight at densities of 50 x 10(3), 100 x 10(3), 300 x 10(3), and 600 x 10(3) cells/dish in medium containing: (1) no treatment (control), (2) LH, or (3) dbcAMP. In Experiment 2, luteal cells from days 5 and 10 of the estrous cycle were transfected with siRNA, which targeted the connexin (Cx) 43 gene. In Experiment 1, progesterone secretion, Cx43 mRNA expression, and the rates of gap junctional intercellular communication (GJIC), were affected by the day of the estrous cycle, cell density, and treatments (LH or dbcAMP). The changes in progesterone secretion were positively correlated with the changes in Cx43 mRNA expression and the rates of GJIC. Cx43 was detected on the luteal cell borders in every culture, and luteal cells expressed 3beta-hydroxysteroid dehydrogenase. In Experiment 2, two Cx43 gene-targeted sequences decreased Cx43 mRNA expression and progesterone production by luteal cells. The changes in Cx43 mRNA expression were positively correlated with changes in progesterone concentration in media. Thus, our data demonstrate a relationship between gap junctions and progesterone secretion that was supported by (1) the positive correlations between progesterone secretion and Cx43 mRNA expression and GJIC of luteal cells and (2) the inhibition of Cx43 mRNA expression by siRNA that resulted in decreased production of progesterone by luteal cells. This suggests that gap junctions may be involved in the regulation of steroidogenesis in the ovine corpus luteum.
authors: Department of Animal and Range Sciences, North Dakota State University, Fargo, North Dakota 58105, USA.
authors: Ewa
authors: E
authors: Borowczyk
authors: Mary Lynn
authors: ML
authors: Johnson
authors: Jerzy J
authors: JJ
authors: Bilski
authors: Magda A
authors: MA
authors: Bilska
authors: Dale A
authors: DA
authors: Redmer
authors: Lawrence P
authors: LP
authors: Reynolds
authors: Anna T
authors: AT
authors: Grazul-Bilska
mesh_terms: D000818:Animals; D001692:Biological Transport; D003994:Bucladesine / Q000494:pharmacology; D002450:Cell Communication; D002478:Cells, Cultured; D018031:Connexin 43 / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D030762:Estrous Cycle / Q000378:metabolism; D005260:Female; D017629:Gap Junctions / Q000502:physiology*; D007090:Image Interpretation, Computer-Assisted; D007150:Immunohistochemistry; D008184:Luteal Cells / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000494:pharmacology; D008856:Microscopy, Fluorescence; D011374:Progesterone / Q000032:analysis / Q000378:metabolism*; D034622:RNA Interference; D012333:RNA, Messenger / Q000032:analysis; D034741:RNA, Small Interfering / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012756:Sheep; D014162:Transfection / Q000379:methods
chemical_list: D018031:Connexin 43; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D011374:Progesterone; D003994:Bucladesine; D007986:Luteinizing Hormone