title: Levobupivacaine hydrochloride and sufentanil have no antimicrobial effect at 25 degrees C in vitro.
abstract: OBJECTIVE
  Levobupivacaine in combination with sufentanil may be used for labour or postoperative regional analgesia. The risk of bacterial growth within these contained solutions for several hours at room temperature is unknown. We investigated the in vitro antimicrobial effect of levobupivacaine and sufentanil against common micro-organisms encountered during regional anaesthesia.
  
  
  METHODS
  Standardized suspensions of Staphylococcus aureus, Staphylococcus epidermidis and Escherichia coli were incubated for 1, 3, 6 and 24 h at 25 degrees C, with saline (as control), sufentanil 0.5 or 0.75 microg mL-1, levobupivacaine hydrochloride 5.6 mg mL-1 and concentrations of 1.4, 2.8 and 5 mg mL-1 of levobupivacaine hydrochloride with sufentanil 0.5 microg mL-1. Colony counts were compared after 24 h incubation at 37 degrees C.
  
  
  RESULTS
  No bacterial growth was observed on any bacterial strain for any solution tested throughout the experiment.
  
  
  CONCLUSIONS
  Our results suggest that solutions of levobupivacaine combined with sufentanil may be used for 24 h at room temperature during regional anaesthesia with no risk of bacterial growth.
authors: Edouard Herriot Hospital, Department of Anaesthesiology and Intensive Care, ISPB Université Lyon 1, EA 3090, Lyon, France.
authors: M
authors: M
authors: Guillier
authors: E
authors: E
authors: Boselli
authors: L
authors: L
authors: Bouvet
authors: J
authors: J
authors: Freney
authors: F N R
authors: FN
authors: Renaud
authors: D
authors: D
authors: Chassard
authors: B
authors: B
authors: Allaouchiche
mesh_terms: D000701:Analgesics, Opioid / Q000494:pharmacology*; D000779:Anesthetics, Local / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002045:Bupivacaine / Q000031:analogs & derivatives / Q000494:pharmacology; D015169:Colony Count, Microbial; D004338:Drug Combinations; D004340:Drug Contamination; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D000077554:Levobupivacaine; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000254:growth & development; D017409:Sufentanil / Q000494:pharmacology*; D013696:Temperature; D013997:Time Factors
chemical_list: D000701:Analgesics, Opioid; D000779:Anesthetics, Local; D000890:Anti-Infective Agents; D004338:Drug Combinations; D000077554:Levobupivacaine; D017409:Sufentanil; D002045:Bupivacaine