[Pilot study of using autologous bone marrow stromal cells and coral to repair canine segmental mandibular defects].
OBJECTIVE
To repair segmental mandibular defects with autologous bone marrow stromal cells (BMSCs) engineered bone.


METHODS
Isolated BMSCs were expanded in vitro and osteogenic induced. In 12 canines, a 3 cm segmental mandibular defect at right mandible was created. 6 canine's defects were repaired with cell-scaffold constructs made from induced BMSCs and coral; others were repaired with coral as control. The engineered bone was evaluated by X-ray, CT, Dual Energy X-ray Absorptiometry (DXA), gross and histological examination, and biomechanical test post-operatively.


RESULTS
Induced BMSCs grew well on coral scaffold. At 12 weeks, X-ray showed more callus formed in experimental group, while evident scaffold duration in control group. At 32 weeks, gross observation, X-ray and CT demonstrated well bony-union in experimental group, while bony-nonunion in control group. Also DXA revealed significantly higher bone mineral density of experimental group than control group. Histologically, mature bone were commonly observed and there were bony healing in experimental group, while fibrous healing occurred in control group. Biomechanical test revealed no significant difference between experimental group and normal group.


CONCLUSIONS
Canine segmental mandibular defects can be repaired with the tissue-engineered bone generated by coral scaffold with autologous osteogenic BMSCs.
Yuan
Jie
J
Department of Plastic and Reconstructive Surgery, 9th People's Hospital affiliated to Shanghai Jiao Tong University Medical School, Shanghai Key Laboratory of Tissue Engineering, Shanghai 200011, China.
Liu
Guang-peng
GP
Chai
Gang
G
Liu
Bo
B
Xu
Feng
F
Cui
Lei
L
Liu
Wei
W
Cao
Yi-lin
YL
D000818:Animals; D037421:Anthozoa; D001854:Bone Marrow Cells / Q000166:cytology; D018786:Bone Substitutes; D018929:Cell Culture Techniques; D004285:Dogs; D008334:Mandible / Q000473:pathology / Q000601:surgery*; D059630:Mesenchymal Stem Cells / Q000166:cytology; D010865:Pilot Projects; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014182:Transplantation, Autologous
D018786:Bone Substitutes