[Construction of the prokaryotic expression vector for human amelogenin gene].
OBJECTIVE
To construct the prokaryotic expression vector for the gene encoding human amelogenin mature peptide.


METHODS
The DNA sequence encoding human amelogenin was inserted into the plasmid pMD 18-T by TA clone technique. pMD 18-T/AMG was digested by BamH I and Xho I. Target DNA was cloned into Prokaryotic expression plasmid pGEX-4T-1 by T4 DNA ligase


RESULTS
Double endonuclease digestion electrophoresis and DNA sequence analysis was conducted to identify the recombined vector pMD 18-T/AMG and pGEX-4T-1/AMG. The results were consistent with the anticipation.


CONCLUSIONS
The prokaryotic expression vector for the gene encoding human amelogenin mature peptide is constructed successfully.
Zhang
Xue-Yang
XY
Faculty of Stomatology, Capital University of Medical Science, Beijing 100050, China.
Zhao
Hua
H
Hu
Fei
F
Zhao
Hong-Yu
HY
Zhang
Jin-Cai
JC
D053523:Amelogenin / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D005822:Genetic Vectors; D006801:Humans; D010957:Plasmids / Q000235:genetics; D011387:Prokaryotic Cells / Q000378:metabolism
D053523:Amelogenin