title: Effects of growth factors on heparin-carrying polystyrene-coated atelocollagen scaffold for articular cartilage tissue engineering.
abstract: The specific aim of our investigation is to study the potential use of a collagen/heparin-carrying polystyrene (HCPS) composite extracellular matrix for articular cartilage tissue engineering. Here, we created a high-performance extracellular matrix (HpECM) scaffold to build an optimal extracellular environment using an HCPS we originally developed, and an atelocollagen honeycomb-shaped-scaffold (ACHMS-scaffold) with a membrane seal. This scaffold was coated with HCPS to enable aggregation of heparin-binding growth factors such as FGF-2 and TGF-beta1 within the scaffold. Three-dimensional culture of rabbit articular chondrocytes within the HpECM-scaffold and subsequent preparation of a tissue-engineered cartilage were investigated. The results showed remarkably higher cell proliferative activity within the HpECM-pretreated-FGF-2 scaffold and the sustenance of phenotype within the HpECM-pretreated-TGF-beta1 scaffold. It was thought that both FGF-2 and TGF-beta1 were stably immobilized in the HpEMC-scaffold since HCPS generated an extracellular environment similar to that of heparan sulfate proteoglycan within the scaffold. These results suggest that an ACHMS-scaffold immobilized with HCPS can be a HpECM for cartilage regeneration to retain the heparin-binding growth factors within the scaffolds.
authors: Department of Orthopaedic Surgery, Surgical Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa 259-1193, Japan. sato-m@is.icc.u-tokai.ac.jp
authors: Masato
authors: M
authors: Sato
authors: Masayuki
authors: M
authors: Ishihara
authors: Miya
authors: M
authors: Ishihara
authors: Nagatoshi
authors: N
authors: Kaneshiro
authors: Genya
authors: G
authors: Mitani
authors: Toshihiro
authors: T
authors: Nagai
authors: Toshiharu
authors: T
authors: Kutsuna
authors: Takashi
authors: T
authors: Asazuma
authors: Makoto
authors: M
authors: Kikuchi
authors: Joji
authors: J
authors: Mochida
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D002358:Cartilage, Articular / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D049109:Cell Proliferation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism; D003094:Collagen / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D006493:Heparin / Q000378:metabolism*; D008422:Materials Testing; D010641:Phenotype; D011137:Polystyrenes / Q000737:chemistry* / Q000378:metabolism; D011817:Rabbits; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
chemical_list: D001672:Biocompatible Materials; D011137:Polystyrenes; D053773:Transforming Growth Factor beta1; C059821:atelocollagen; D016222:Fibroblast Growth Factor 2; D006493:Heparin; D003094:Collagen