Sensitivity of Chlamydia suis to cathelicidin peptides.
Nine Chlamydia suis isolates, obtained from pigs with conjunctivitis, were molecularly characterized by ompA sequencing and their in vitro susceptibility to six cathelicidin peptides (SMAP-29, BAC-7, BMAP-27, BMAP-27, BMAP-28, PG-1, LL-37) determined in cell culture. SMAP-29 was the most active peptide, reducing the intracellular inclusion number by > or =50% at a concentration of 10 microg/ml (3 microM) in six of the nine isolates tested. Three molecularly identical isolates were insensitive at a concentration as high as 80 microg/ml (25 microM). Of the remaining cathelicidin peptides tested, BAC-7 and BMAP-27 were active against six C. suis isolates at a concentration of 80 microg/ml (25 and 26 microM, respectively). Cathelicidins LL-37 and PG-1 did not show any anti-chlamydial activity at 80 microg/ml.
Donati
Manuela
M
Sezione di Microbiologia DMCSS, Policlinico S. Orsola, Università di Bologna, Via Massarenti 9, 40138 Bologna, Italy.
Di Francesco
Antonietta
A
Gennaro
Renato
R
Benincasa
Monica
M
Magnino
Simone
S
Pignanelli
Salvatore
S
Shurdhi
Alisa
A
Moroni
Alessandra
A
Mazzoni
Claudio
C
Merialdi
Giuseppe
G
Baldelli
Raffaella
R
Cevenini
Roberto
R
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000494:pharmacology*; D054804:Cathelicidins; D002417:Cattle; D002460:Cell Line; D002689:Chlamydia / Q000145:classification* / Q000187:drug effects*; D002522:Chlorocebus aethiops; D006801:Humans; D013552:Swine
D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D054804:Cathelicidins