Secretion of the glucose-regulated selenoprotein SEPS1 from hepatoma cells.
SEPS1 (also called selenoprotein S, SelS, Tanis or VIMP) is a selenoprotein, localized predominantly in the ER membrane and also on the cell surface. In this report, we demonstrate that SEPS1 protein is also secreted from hepatoma cells but not from five other types of cells examined. The secretion can be abolished by the ER-Golgi transport inhibitor Brefeldin A and by the protein synthesis inhibitor cycloheximide. Using a sandwich ELISA, SEPS1 was detected in the sera of 65 out of 209 human subjects (31.1%, average=15.7+/-1.1 ng/mL). Fractionation of human serum indicated that SEPS1 was associated with LDL and possibly with VLDL. The function of plasma SEPS1 is unclear but may be related to lipoprotein metabolism.
Gao
Yuan
Y
Division of Textile and Fibre Technology, Commonwealth Scientific and Industrial Research Organization, Bayview Ave, Clayton 3168, Australia. yuan.gao@csiro.au
Pagnon
Joanne
J
Feng
Helen C
HC
Konstantopolous
Nicky
N
Jowett
Jeremy B M
JB
Walder
Ken
K
Collier
Greg R
GR
D041721:3T3-L1 Cells; D000818:Animals; D019556:COS Cells; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood; D008113:Liver Neoplasms / Q000378:metabolism*; D008565:Membrane Proteins / Q000097:blood / Q000378:metabolism*; D051379:Mice; D051381:Rats; D051140:Selenoproteins / Q000097:blood / Q000378:metabolism*
D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D008565:Membrane Proteins; C487710:SELENOS protein, human; D051140:Selenoproteins; C457119:tanis protein, Psammomys obesus