title: Immunohistochemical localization of angiotensin II receptor types 1 and 2 in the mesenteric artery from spontaneously hypertensive rats.
abstract: Angiotensin II plays a crucial role in the control of blood pressure, acting at AT1 or AT2 receptors, and can act as a potent vasoconstrictor of the peripheral vasculature inducing hypertrophy, hyperplasia, or both, in resistance arteries. The aim of the present study was to investigate whether the pattern of distribution of angiotensin AT1 and AT2 receptors on mesenteric artery sections differs in spontaneously hypertensive rats (SHR) versus their respective controls (Wistar-Kyoto [WKY] rats). Immunohistochemistry using anti-AT1 or anti-AT2 antibodies was performed on perfused-fixed/paraffin-embedded mesenteric arteries from SHR and WKY rats. 3,3'-Diaminobenzidine tetrahydrochloride (DAB; activated by hydrogen peroxide) staining revealed distinct AT1 and AT2 labeling of all artery layers (adventitia, media and intima) from WKY rats, whereas in SHR an abundant AT1 labeling was found in both intima and adventitia and a sparser labeling in the media. There was a vast reduction of AT2 labeling throughout all layers. These results suggest a crucial role for AT2 receptors in the pathogenesis of hypertension.
authors: Serviço de Farmacologia, REQUIMTE, Faculdade de Farmácia, Universidade do Porto, 4050-047 Porto, Portugal. cdiniz@ff.up.pt
authors: Carmen
authors: C
authors: Diniz
authors: Sandra
authors: S
authors: Leal
authors: Karen
authors: K
authors: Logan
authors: Carolina
authors: C
authors: Rocha-Pereira
authors: Ana Sofia
authors: AS
authors: Soares
authors: Eduardo
authors: E
authors: Rocha
authors: Jorge
authors: J
authors: Gonçalves
authors: Paula
authors: P
authors: Fresco
mesh_terms: D000818:Animals; D006973:Hypertension / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008638:Mesenteric Arteries / Q000737:chemistry / Q000378:metabolism*; D016612:Paraffin Embedding; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D044140:Receptor, Angiotensin, Type 1 / Q000032:analysis* / Q000378:metabolism; D044139:Receptor, Angiotensin, Type 2 / Q000032:analysis* / Q000378:metabolism
chemical_list: D044140:Receptor, Angiotensin, Type 1; D044139:Receptor, Angiotensin, Type 2