Characterization of mycobacteria isolated from bovines by PRA-targetting hsp 65 gene region.
Bovine tuberculosis caused by the bacterium Mycobacterium bovis is a major infectious disease of animals and has zoonotic importance for humans. Even though the incidence is believed to be very low in India, human tuberculosis caused by M. bovis has been increasingly recognized in many other countries of the world. As differentiation of mycobacterial species take long time, a method for the rapid identification of mycobacteria isolated from bovine samples to the species level was used, which is based on polymerase chain reaction (PCR) of the gene encoding for the 65-kD protein followed by restriction analysis. The method involves restriction enzyme analysis of PCR products obtained with primers common to all mycobacteria and generate M. tuberculosis complex specific pattern. PRA was performed on 33 bovine isolates of which 90.9% (30/33) isolates were identified clearly as M. tuberculosis complex, M. fortuitum, M. phlei and M. smegmatis using restriction enzyme Hae III.
Parashar
Deepti
D
Deptt of Microbiology and Molecular Biology, National JALMA Institute for Leprosy and Other Mycobacterial Diseases (ICMR), Tajganj, Agra 282001.
Srivastava
R K
RK
Chauhan
D S
DS
Sharma
V D
VD
Singh
Mradula
M
Lavania
Mallika
M
Chauhan
Aradhana
A
Bhatia
A K
AK
Katoch
V M
VM
D000818:Animals; D001426:Bacterial Proteins / Q000145:classification* / Q000235:genetics; D002417:Cattle; D018834:Chaperonin 60; D018833:Chaperonins / Q000145:classification* / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis; D009168:Mycobacterium phlei / Q000145:classification* / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics; D009170:Nontuberculous Mycobacteria / Q000145:classification* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D014380:Tuberculosis, Bovine / Q000145:classification*
D001426:Bacterial Proteins; D018834:Chaperonin 60; D004269:DNA, Bacterial; C074119:heat-shock protein 65, Mycobacterium; D018833:Chaperonins