title: Changes in glycosylation of vitronectin modulate multimerization and collagen binding during liver regeneration.
abstract: Elucidating the mechanisms and factors regulating multimerization is biologically important in order to modulate the biological activities of functional proteins, especially adhesive proteins in the extracellular matrix (ECM). Vitronectin (VN) is a multifunctional glycoprotein present in plasma and ECM. Linkage of cellular adhesion and fibrinolysis by VN plays an essential role during tissue remodeling. Our previous study determined that the collagen-binding activity of VN was markedly enhanced with the decreased glycosylation during liver regeneration. This study demonstrated how alternations of glycans modulate the biological activity of VN. Human and rat VNs were used because of their similarities in structure and activities. The binding affinity of human VN to immobilized collagen was shown to be higher at pH 4.5 than at 7.5, at 37 degrees C than at 4 degrees C. Sedimentation velocity studies indicated that the greater the multimerization of human VN, the better it bound to collagen. The results indicate that the collagen binding of VN was modulated through its multimerization. Stepwise trimming of glycan with various exoglycosidases increased both the multimer size and the collagen binding of human VN, indicating that they are modulated by changes in glycosylation. The multimer sizes of VN purified from plasma of partially hepatectomized (PH) rats and sham-operated (SH) rats increased by about 45 and 31%, respectively, compared with those of nonoperated (NO) rats. In accordance with this, PH-VN exhibited remarkably enhanced collagen binding than SH-VN and NO-VN on surface plasmon resonance. In the PH rat sera, the multimer VN was increased in both amount and size compared with those in SH- and NO-sera. The results demonstrate that glycan alterations during tissue remodeling induce increased multimerization state to enhance the biological activity of VN.
authors: Graduate school of Humanities and Sciences and The Glycoscience Institute, Ochanomizu University, 2-1-1 Otsuka, Bunkyo-ku, Tokyo, 112-8610 Japan.
authors: Kotone
authors: K
authors: Sano
authors: Kimie
authors: K
authors: Asanuma-Date
authors: Fumio
authors: F
authors: Arisaka
authors: Shunji
authors: S
authors: Hattori
authors: Haruko
authors: H
authors: Ogawa
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D003094:Collagen / Q000737:chemistry*; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008115:Liver Regeneration; D008297:Male; D008954:Models, Biological; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar; D020349:Surface Plasmon Resonance; D019096:Vitronectin / Q000096:biosynthesis* / Q000737:chemistry*
chemical_list: D019096:Vitronectin; D003094:Collagen; D006026:Glycoside Hydrolases