Enhancing salt tolerance in a crop plant by overexpression of glyoxalase II.
Earlier we have shown the role of glyoxalase overexpression in conferring salinity tolerance in transgenic tobacco. We now demonstrate the feasibility of same in a crop like rice through overproduction of glyoxalase II. The rice glyoxalase II was cloned in pCAMBIA1304 and transformed into rice (Oryza sativa cv PB1) via Agrobacterium. The transgenic plants showed higher constitutive activity of glyoxalase II that increased further upon salt stress, reflecting the upregulation of endogenous glyoxalase II. The transgenic rice showed higher tolerance to toxic concentrations of methylglyoxal (MG) and NaCl. Compared with non-transgenics, transgenic plants at the T1 generation exhibited sustained growth and more favorable ion balance under salt stress conditions.
Singla-Pareek
Sneh L
SL
Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110 067, India. sneh@icgeb.res.in
Yadav
Sudesh Kumar
SK
Pareek
Ashwani
A
Reddy
M K
MK
Sopory
S K
SK
D000222:Adaptation, Physiological / Q000235:genetics*; D015139:Blotting, Southern; D015153:Blotting, Western; D017931:DNA Primers; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D018014:Gene Transfer Techniques; D012275:Oryza / Q000187:drug effects / Q000235:genetics*; D030821:Plants, Genetically Modified; D011765:Pyruvaldehyde / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012231:Rhizobium / Q000235:genetics; D012965:Sodium Chloride / Q000494:pharmacology*; D013869:Thiolester Hydrolases / Q000235:genetics*; D000099301:Hydroxyacylglutathione Hydrolase
D017931:DNA Primers; D011765:Pyruvaldehyde; D012333:RNA, Messenger; D012965:Sodium Chloride; D013869:Thiolester Hydrolases; D000099301:Hydroxyacylglutathione Hydrolase