title: RNA recognition mechanism of eukaryote tRNA (m7G46) methyltransferase (Trm8-Trm82 complex).
abstract: Yeast tRNA (m(7)G46) methyltransferase contains two protein subunits (Trm8 and Trm82). To address the RNA recognition mechanism of the Trm8-Trm82 complex, we investigated methyl acceptance activities of eight truncated yeast tRNA(Phe) transcripts. Both the D-stem and T-stem structures were required for efficient methyl-transfer. To clarify the role of the D-stem structure, we tested four mutant transcripts, in which tertiary base pairs were disrupted. The tertiary base pairs were important but not essential for the methyl-transfer to yeast tRNA(Phe) transcript, suggesting that these base pairs support the induced fit of the G46 base into the catalytic pocket.
authors: Department of Applied Chemistry, Faculty of Engineering, Ehime University, Bunkyo 3, Matsuyama 790-8577, Japan.
authors: Keisuke
authors: K
authors: Matsumoto
authors: Takashi
authors: T
authors: Toyooka
authors: Chie
authors: C
authors: Tomikawa
authors: Anna
authors: A
authors: Ochi
authors: Yoshitaka
authors: Y
authors: Takano
authors: Naoyuki
authors: N
authors: Takayanagi
authors: Yaeta
authors: Y
authors: Endo
authors: Hiroyuki
authors: H
authors: Hori
mesh_terms: D001665:Binding Sites; D009690:Nucleic Acid Conformation; D012360:RNA, Transfer, Phe / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*; D012359:tRNA Methyltransferases / Q000737:chemistry*
chemical_list: D012360:RNA, Transfer, Phe; D029701:Saccharomyces cerevisiae Proteins; D012359:tRNA Methyltransferases; C036814:tRNA (guanine-N7-)-methyltransferase