title: Application of protein-fragment complementation assays in cell biology.
abstract: We have developed a general experimental strategy that enables the quantitative detection of dynamic protein-protein interactions in intact living cells, based on protein-fragment complementation assays (PCAs). In this method, protein-protein interactions are coupled to refolding of enzymes from cognate fragments where reconstitution of enzyme activity acts as the detector of a protein interaction. Here we discuss the application of PCA to different aspects of cell biology.
authors: Département de Biochimie, Université de Montréal, Succursale Center-Ville, Montréal, QC, Canada.
authors: Ingrid
authors: I
authors: Remy
authors: Stephen W
authors: SW
authors: Michnick
mesh_terms: D001669:Biochemical Phenomena; D001671:Biochemistry; D003585:Cell Biology; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins