Preliminary antigenic characterisation of an adult worm vomit preparation of Fasciola hepatica by infected human sera.
Fascioliasis is an emerging/re-emerging vector-borne disease with the widest known distribution. Approximately 17 million people are infected around the world, being the Andean region the most affected area. There is an important necessity to develop sensitive and specific diagnostic tools to treat patients early and to avoid complications. In this paper we evaluated the immune response of infected humans against two antigenic preparations: the total soluble extract (FhTSE) and the adult worm vomit (FhAWV) in order to identify antigenic fractions specific for Fasciola hepatica. Both preparations were processed by SDS-PAGE and Western blot with human sera with fascioliasis (F), other parasitosis and healthy individuals. In the immunoblot of FhTSE, sera F recognised 16 bands with MW between eight and 110 kDa, from which those of 8, 9, 10, 38, 45 and 57 kDa were specific. In the preparation FhAWV, sera F recognised nine bands with MW from eight to 85 kDa, from which those of 8, 12, 15 and 24 kDa were specific. Some bands of cross-reaction were evident with sera from patients with other parasitoses, more frequent with the FhTSE. Bands within the MW mentioned, particularly that of eight kDa, have been shown to be specific by others, and deserve additional characterisation for their potential use in immunodiagnosis.
De Almeida
María Alejandra
MA
Departamento de Parasitología, Facultad de Ciencias de la Salud, Universidad de Carabobo, Valencia, Venezuela.
Ferreira
María Benardetti
MB
Planchart
Sandra
S
Terashima
Angélica
A
Maco
Vicente
V
Marcos
Luis
L
Gotuzzo
Eduardo
E
Sánchez
Elizabeth
E
Náquira
César
C
Scorza
José Vicente
JV
Incani
Renzo Nino
RN
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology; D015153:Blotting, Western; D016022:Case-Control Studies; D002417:Cattle; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D005210:Fasciola hepatica / Q000276:immunology*; D005211:Fascioliasis / Q000175:diagnosis*; D015801:Helminth Proteins / Q000276:immunology*; D006801:Humans
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins