title: The eyes of anencephalic babies: a morphological and immunohistochemical evaluation.
abstract: This study studied the eyes of three anencephalic stillborns to evaluate whether brain degeneration affected eye development and/or survival. The study encompassed histology, scanning electronmicroscopy, and immunocytochemistry. The corneae were otherwise normal except for the presence of blood vessels in the stroma and the posterior surface of the cornea demonstrated wrinkles. Synaechia was present and the lens had occasional vacuolated cells. The retinae had normal layers in most regions except the center where fibroblasts infiltration was observed. The optic nerve was atypical and composed of aggregates of disoriented fibroblasts and disoriented nerve fibers. Anti-cleaved caspase 3 immunocytochemistry revealed only few positive dying cells in the visual cell layer. Antineurofilament 200 reactions demonstrated positive ganglion cells even in the anencephalic eyes. The choroids in anencephaly had more VEGF positive sites, indicating vascularization in both control and anencephalic eyes. If the brains degenerate before retinal maturation, then such degenerations may not have an effect on subsequent retinal development except for the degeneration of the nerve fiber layer. If the brains degenerate after retinal maturation, then the survival of the retinae does not appear to rely on its linkage with the brain at birth, again apart from the degeneration of nerve fibers.
authors: Department of Ophthalmology and Visual Sciences, Chinese University of Hong Kong, Shatin, N.T., Hong Kong.
authors: Winnie Wai-Ying
authors: WW
authors: Li
authors: Gang
authors: G
authors: Lu
authors: Chi-Pui
authors: CP
authors: Pang
authors: Dennis Shun-Chiu
authors: DS
authors: Lam
authors: David T
authors: DT
authors: Yew
mesh_terms: D000757:Anencephaly / Q000473:pathology / Q000648:ultrastructure*; D016022:Case-Control Studies; D003315:Cornea / Q000473:pathology / Q000648:ultrastructure; D005123:Eye / Q000473:pathology / Q000648:ultrastructure*; D006801:Humans; D007150:Immunohistochemistry; D007231:Infant, Newborn; D007498:Iris / Q000473:pathology / Q000648:ultrastructure; D007908:Lens, Crystalline / Q000473:pathology / Q000648:ultrastructure; D012160:Retina / Q000473:pathology / Q000648:ultrastructure*