title: Analysis of glycans in glycoproteins by diffusion-ordered nuclear magnetic resonance spectroscopy.
abstract: Enzymatically cleaved glycans from sub-milligram quantities of erythropoietin (EPO) and ovalbumin have been analyzed, without further purification, by two-dimensional diffusion-ordered nuclear magnetic resonance spectroscopy. At NMR sample concentrations below 50 micromol L-1 the major components of the oligosaccharide fractions could be distinguished by their anomeric proton chemical shift and their size-dependent diffusion coefficients.
authors: Institute of Analytical Chemistry, Johannes Kepler University, Altenberger Strasse 69, 4040, Linz, Austria.
authors: Karin
authors: K
authors: Ortner
authors: Veeramuthu N
authors: VN
authors: Sivanandam
authors: Wolfgang
authors: W
authors: Buchberger
authors: Norbert
authors: N
authors: Müller
mesh_terms: D004058:Diffusion; D004921:Erythropoietin / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010047:Ovalbumin / Q000737:chemistry; D011134:Polysaccharides / Q000032:analysis*
chemical_list: D006023:Glycoproteins; D011134:Polysaccharides; D004921:Erythropoietin; D010047:Ovalbumin