title: [Study of loss of heterozygosity in oligodendroglial tumors by real-time quantitative polymerase chain reaction-based microsatellite analysis].
abstract: OBJECTIVE
  To study the loss of heterozygosity (LOH) at chromosomes 1p or 19q in oligodendroglial tumors.
  
  
  METHODS
  Twenty-eight cases of oligodendroglial tumors were enrolled into the study. Real-time quantitative polymerase chain reaction-based microsatellite analysis was performed on paraffin-embedded tumor tissues in order to study the status of chromosomes 1p and 19q.
  
  
  RESULTS
  Among the 28 cases of oligodendroglial tumors, 24 cases (85.7%) showed 1p LOH, while 18 cases (64.3%) showed 19q LOH and 17 cases (60.7%) showed LOH of both 1p and 19q. LOH at 1p or 19q was present in 25 (89.3%) of the 28 cases.
  
  
  CONCLUSIONS
  Real-time quantitative polymerase chain reaction-based microsatellite analysis is a rapid and specific way in detecting LOH in paraffin-embedded tumor tissues. LOH at 1p or 19q is present in majority of the oligodendroglial tumors studied.
authors: Department of Pathology, Xuanwu Hospital, Capital University of Medical Sciences, Beijing 100053, China.
authors: Shu-kun
authors: SK
authors: Zhang
authors: De-hong
authors: DH
authors: Lu
authors: Yue-shan
authors: YS
authors: Piao
authors: Yan-ning
authors: YN
authors: Cai
authors: Qing-zhong
authors: QZ
authors: Xu
mesh_terms: D000328:Adult; D001932:Brain Neoplasms / Q000235:genetics*; D002878:Chromosomes, Human, Pair 1; D002888:Chromosomes, Human, Pair 19; D005260:Female; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D009837:Oligodendroglioma / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*