title: Abnormal expression, highly efficient detection and novel truncations of midkine in human tumors, cancers and cell lines.
abstract: We detected aberrant Midkine (MK) expressions in human insulinoma and pancreatic cancer tissues by immunohistochemistry, revealing its potential role in tumorigenesis/carcinogenesis. With a nested-touchdown PCR program we were able to detect the tMK in all human tumor/cancer tissues and cancer/tumor cell lines. Detection of MK in the peripheral cells and precancerous lesions implies its potential for early cancer/tumor diagnosis. Furthermore, we have discovered two novel truncations of the MK, tMKB and tMKC, respectively, in the disease specimens. Our data not only provide an efficient methodology potentially for clinical application but also shed light on the molecular mechanism underlying the role for MK in tumorigenesis/carcinogenesis.
authors: Department of Biomedical Sciences, Xiamen University, Xiamen City, Fujian Province 361005, China. tapoing@yahoo.com.cn
authors: Ping
authors: P
authors: Tao
authors: Donghui
authors: D
authors: Xu
authors: Shuibin
authors: S
authors: Lin
authors: Gao-Liang
authors: GL
authors: Ouyang
authors: Youde
authors: Y
authors: Chang
authors: Qin
authors: Q
authors: Chen
authors: Yuanyang
authors: Y
authors: Yuan
authors: Xinming
authors: X
authors: Zhuo
authors: Qicong
authors: Q
authors: Luo
authors: Jie
authors: J
authors: Li
authors: Baoan
authors: B
authors: Li
authors: Lingjuan
authors: L
authors: Ruan
authors: Qifu
authors: Q
authors: Li
authors: Zhixing
authors: Z
authors: Li
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D045744:Cell Line, Tumor; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007340:Insulinoma / Q000235:genetics* / Q000378:metabolism; D000078792:Midkine; D008969:Molecular Sequence Data; D009154:Mutation; D010179:Pancreas / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D016207:Cytokines; D000078792:Midkine