title: Diabetic rats supplemented with L-glutamine: a study of immunoreactive myosin-V myenteric neurons and the proximal colonic mucosa.
abstract: We studied the neuronal density and size of myenteric neurons and the epithelial cell proliferation and crypt depth of the proximal colon in diabetic Wistar rats after supplementing them with L-glutamine (1%). The animals were divided into five groups: untreated normoglycemic (UN), L-glutamine-treated normoglycemic (NG), untreated diabetic (UD), and L-glutamine-treated diabetics 4 days (DG4) and 45 days (DG45) days after the onset of diabetes. We observed a reduction of 52.7% and 50.44% in the neuronal density of the proximal colon of the UD group compared to the UN and NG groups, respectively (P<0.05). The neuronal density found for the DG4 (32.8%) and DG45 (28.6%) groups was higher than that of the UD group (P>0.05). There were no significant differences (P>0.05) when the data relative to the area of the myenteric neuron cell bodies, metaphasic index, and crypt depth in the proximal colon were compared among experimental groups.
authors: Department of Morpho-Physiological Sciences (DCM/UEM), State University of Maringá, Av. Colombo 5790, 87020-90, Maringá, PR, Brazil.
authors: Cristiano Massao
authors: CM
authors: Tashima
authors: Eleandro Aparecido
authors: EA
authors: Tronchini
authors: Renata Virginia Fernandes
authors: RV
authors: Pereira
authors: Roberto Barbosa
authors: RB
authors: Bazotte
authors: Jacqueline Nelisis
authors: JN
authors: Zanoni
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D003106:Colon / Q000187:drug effects* / Q000294:innervation / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000150:complications* / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D003929:Diabetic Neuropathies / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D004032:Diet; D005973:Glutamine / Q000008:administration & dosage / Q000494:pharmacology*; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000294:innervation / Q000378:metabolism; D008297:Male; D009197:Myenteric Plexus / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D024701:Myosin Type V / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D000975:Antioxidants; D005973:Glutamine; D024701:Myosin Type V