title: Iron and exercise induced alterations in antioxidant status. Protection by dietary milk proteins.
abstract: Lipid peroxidation stress induced by iron supplementation can contribute to the induction of gut lesions. Intensive sports lead to ischemia reperfusion, which increases free radical production. Athletes frequently use heavy iron supplementation, whose effects are unknown. On the other hand, milk proteins have in vitro antioxidant properties, which could counteract these potential side effects. The main aims of the study were: (1) to demonstrate the effects of combined exercise training (ET) and iron overload on antioxidant status; (2) to assess the protective properties of casein in vivo; (3) to study the mechanisms involved in an in vitro model. Antioxidant status was assessed by measuring the activity of antioxidant enzymes (superoxide dismutase (SOD); glutathione peroxidase (GSH-Px)), and on the onset of aberrant crypts (AC) in colon, which can be induced by lipid peroxidation. At day 30, all ET animals showed an increase in the activity of antioxidant enzymes, in iron concentration in colon mucosa and liver and in the number of AC compared to untrained rats. It was found that Casein's milk protein supplementation significantly reduced these parameters. Additional information on protective effect of casein was provided by measuring the extent of TBARS formation during iron/ascorbate-induced oxidation of liposomes. Free casein and casein bound to iron were found to significantly reduce iron-induced lipid peroxidation. The results of the overall study suggest that Iron supplementation during intensive sport training would decrease anti-oxidant status. Dietary milk protein supplementation could at least partly prevent occurrence of deleterious effects to tissue induced by iron overload.
authors: Laboratoire de Physiologie digestive et nutritionnelle, CHU Caen, Caen, France.
authors: Gautier
authors: G
authors: Zunquin
authors: Vincent
authors: V
authors: Rouleau
authors: Said
authors: S
authors: Bouhallab
authors: Francois
authors: F
authors: Bureau
authors: Denis
authors: D
authors: Theunynck
authors: Pierre
authors: P
authors: Rousselot
authors: Pierre
authors: P
authors: Arhan
authors: Dominique
authors: D
authors: Bougle
mesh_terms: D000818:Animals; D002364:Caseins / Q000008:administration & dosage* / Q000378:metabolism; D003106:Colon / Q000378:metabolism / Q000473:pathology; D004032:Diet; D005979:Glutathione Peroxidase / Q000378:metabolism; D007501:Iron / Q000008:administration & dosage* / Q000378:metabolism; D019190:Iron Overload; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000378:metabolism; D010805:Physical Conditioning, Animal; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D002364:Caseins; D017392:Thiobarbituric Acid Reactive Substances; D007501:Iron; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase