title: Crab digestive lipase acting at high temperature: purification and biochemical characterization.
abstract: In recent years, recovery and characterization of enzymes from fish and aquatic invertebrates have taken place and this had led to the emergence of some interesting new applications of these enzymes. However, much less is known about lipases from crustaceans. A lipolytic activity was located in the crab digestive glands (hepatopancreas), from which a crab digestive lipase (CDL) was purified. Pure CDL has a molecular mass of 65kDa as determined by SDS/PAGE analysis. Unlike known digestive lipases, CDL displayed its maximal activity on long and short-chain triacylglycerols at a temperature of 60 degrees C. A specific activity of 500U/mg or 130U/mg was obtained with TC(4) or olive oil as substrate, respectively. Only 10% of the maximal activity was detected at 37 degrees C. The enzyme retained 80% of its maximal activity when incubated during 10 min at 60 degrees C, and was completely inactivated at a temperature higher than 65 degrees C. Interestingly, neither colipase, nor bile salts were detected in the crab hepatopancreas. Which suggests that colipase evolved in invertebrates simultaneously with the appearance of an exocrine pancreas and a true liver which produce bile salts. No similarity between the 13 N-terminal amino acid residues of CDL was found with those of known other digestive lipases.
authors: Laboratoire de Biochimie et de Génie Enzymatique des Lipases, ENIS, BPW 3038, Sfax, Tunisia.
authors: Slim
authors: S
authors: Cherif
authors: Ahmed
authors: A
authors: Fendri
authors: Nabil
authors: N
authors: Miled
authors: Heykel
authors: H
authors: Trabelsi
authors: Hafedh
authors: H
authors: Mejdoub
authors: Youssef
authors: Y
authors: Gargouri
mesh_terms: D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism; D003386:Brachyura / Q000201:enzymology*; D003089:Colipases / Q000378:metabolism; D043143:Hepatopancreas / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000737:chemistry* / Q000302:isolation & purification*; D013696:Temperature; D013997:Time Factors
chemical_list: D001647:Bile Acids and Salts; D003089:Colipases; D008049:Lipase