title: [The effect of NT4-NAP fusion gene transfection on photoprotection of rabbit iris pigment epithelium cells].
abstract: OBJECTIVE
  To investigate the effect of infection with adeno-associated virus (AAV) vector containing NT4-NAP fusion gene on photoprotection of rabbit iris pigment epithelium cells (IPECs).
  
  
  METHODS
  rAAV-GFP and rAAV-NAP (containing NT4-NAP fusion gene) were constructed; rabbit IPECs were cultured and infected with rAAV-GFP and rAAV-NAP; transfection of viruses was detected by GFP fluorescence expression; NAP protect rabbit iris pigment epithelium from light stimulation was evaluated by MTT and flow cytometry.
  
  
  RESULTS
  Rabbit IPECs expressed the GFP fluorescence; comparing with control cells, IPECs transfected with rAAV-NAP remained normal proliferation and showed lower apoptosis percentage after ultra-violet stimulation.
  
  
  CONCLUSIONS
  rAAV-NAP constructed in the study can infect rabbit IPECs, and NAP may protect rabbit IPECs from ultra-violet damage.
authors: Department of Ophthalmology, the Second Hospital, Xi'an Jiaotong University, Xi'an 710004, China. shehuaning@126.com
authors: Hua-ning
authors: HN
authors: She
authors: Nai-xue
authors: NX
authors: Sun
authors: Yu-ping
authors: YP
authors: Zheng
authors: Yan-long
authors: YL
authors: Quan
authors: Zhen-ping
authors: ZP
authors: Cao
authors: Xiao-hua
authors: XH
authors: Wang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D050939:Gene Fusion; D005822:Genetic Vectors; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009842:Oligopeptides / Q000235:genetics*; D010857:Pigment Epithelium of Eye / Q000166:cytology*; D011817:Rabbits; D014162:Transfection
chemical_list: D009419:Nerve Tissue Proteins; D009842:Oligopeptides; C425904:davunetide