title: [Construction of the prokaryotic expression vector for human amelogenin gene].
abstract: OBJECTIVE
  To construct the prokaryotic expression vector for the gene encoding human amelogenin mature peptide.
  
  
  METHODS
  The DNA sequence encoding human amelogenin was inserted into the plasmid pMD 18-T by TA clone technique. pMD 18-T/AMG was digested by BamH I and Xho I. Target DNA was cloned into Prokaryotic expression plasmid pGEX-4T-1 by T4 DNA ligase
  
  
  RESULTS
  Double endonuclease digestion electrophoresis and DNA sequence analysis was conducted to identify the recombined vector pMD 18-T/AMG and pGEX-4T-1/AMG. The results were consistent with the anticipation.
  
  
  CONCLUSIONS
  The prokaryotic expression vector for the gene encoding human amelogenin mature peptide is constructed successfully.
authors: Faculty of Stomatology, Capital University of Medical Science, Beijing 100050, China.
authors: Xue-Yang
authors: XY
authors: Zhang
authors: Hua
authors: H
authors: Zhao
authors: Fei
authors: F
authors: Hu
authors: Hong-Yu
authors: HY
authors: Zhao
authors: Jin-Cai
authors: JC
authors: Zhang
mesh_terms: D053523:Amelogenin / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D005822:Genetic Vectors; D006801:Humans; D010957:Plasmids / Q000235:genetics; D011387:Prokaryotic Cells / Q000378:metabolism
chemical_list: D053523:Amelogenin