The reactive metabolite target protein database (TPDB)--a web-accessible resource.
BACKGROUND
The toxic effects of many simple organic compounds stem from their biotransformation to chemically reactive metabolites which bind covalently to cellular proteins. To understand the mechanisms of cytotoxic responses it may be important to know which proteins become adducted and whether some may be common targets of multiple toxins. The literature of this field is widely scattered but expanding rapidly, suggesting the need for a comprehensive, searchable database of reactive metabolite target proteins.


METHODS
The Reactive Metabolite Target Protein Database (TPDB) is a comprehensive, curated, searchable, documented compilation of publicly available information on the protein targets of reactive metabolites of 18 well-studied chemicals and drugs of known toxicity. TPDB software enables i) string searches for author names and proteins names/synonyms, ii) more complex searches by selecting chemical compound, animal species, target tissue and protein names/synonyms from pull-down menus, and iii) commonality searches over multiple chemicals. Tabulated search results provide information, references and links to other databases.


CONCLUSIONS
The TPDB is a unique on-line compilation of information on the covalent modification of cellular proteins by reactive metabolites of chemicals and drugs. Its comprehensiveness and searchability should facilitate the elucidation of mechanisms of reactive metabolite toxicity. The database is freely available at http://tpdb.medchem.ku.edu/tpdb.html.
Hanzlik
Robert P
RP
Department of Medicinal Chemistry, University of Kansas, Lawrence, KS 66045, USA. rhanzlik@ku.edu
Koen
Yakov M
YM
Theertham
Bhargav
B
Dong
Yinghua
Y
Fang
Jianwen
J
D001665:Binding Sites; D003628:Database Management Systems; D030562:Databases, Protein; D016503:Drug Delivery Systems / Q000379:methods*; D016247:Information Storage and Retrieval / Q000379:methods*; D020407:Internet; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D014584:User-Computer Interface
D011506:Proteins