Lipopolythioureas: a new non-cationic system for gene transfer.
A DNA-transfection protocol has been developed that makes use of thiourea non-cationic synthetic lipid, N-[1,3-bis(carbamothioylamino)propan-2-yl]-2-(dialkycarbamoylmethoxy)acetamide. It was found that these new compounds could be formulated without helper lipid and that the N-decanoyl and N-lauryl derivatives transfected B16 cells in the presence of serum with an efficiency at the same level as cationic lipids, under identical conditions. In vivo transfection using intratumoral injection was also investigated. It was found that compounds 18c and 19 showed an efficiency of the same magnitude as naked DNA and cationic lipid.
Leblond
Jeanne
J
Inserm, U640, Paris, F-75006, CNRS, UMR8151, Paris, F-75006, Ecole Nationale Supérieure de Chimie de Paris, Paris, France.
Mignet
Nathalie
N
Largeau
Céline
C
Spanedda
Maria-Vittoria
MV
Seguin
Johanne
J
Scherman
Daniel
D
Herscovici
Jean
J
D000818:Animals; D002412:Cations; D004247:DNA / Q000008:administration & dosage* / Q000737:chemistry; D018014:Gene Transfer Techniques; D008055:Lipids / Q000138:chemical synthesis / Q000737:chemistry*; D008081:Liposomes; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008546:Melanoma, Experimental / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D010957:Plasmids / Q000008:administration & dosage; D013890:Thiourea / Q000138:chemical synthesis / Q000737:chemistry*; D014162:Transfection; D014407:Tumor Cells, Cultured
D002412:Cations; D008055:Lipids; D008081:Liposomes; D004247:DNA; D008156:Luciferases; D013890:Thiourea