title: [Distribution of Haemopuilus actinomycetemcomitans in chronic periodontitis patients and periodontally healthy subjects].
abstract: OBJECTIVE
  To investigate the prevalence of H. actinomycetemcomitans in Chinese chronic periodontitis (CP) patients and periodontally healthy adults.
  
  
  METHODS
  116 chronic periodontitis patients and 111 periodontally healthy adults were included. In each CP patient, subgingival plaque samples were collected from two sites of different molars with the greatest probing depth (PD) and one periodontally healthy site (PD < or =3 mm). The samples of periodontally healthy adults were obtained from the mesio-buccal site of one first upper molar. Bacteria DNA were extracted for detection of H. actinomycetemcomitans by 16S rRNA PCR.
  
  
  RESULTS
  The prevalence for H. actinomycetemcomitans of diseased sites (33.62%) was significantly higher than that of healthy sites from CP patients (0.86%) and the periodontally sites (0.90%) (P < 0.01). No significant difference was observed between male and female CP patients (P > 0.05). A decreasing trend of H. actinomycetemcomitans was observed as the age increased. And the pocket depth and clinical attachment losswas associated with the occurrence of H. actinomycetemcomitans in a positive mode. And H. actinomycetemcomitans was more often detected in the bleeding sites on probing.
  
  
  CONCLUSIONS
  H. actinomycetemcomitans was more frequently detected in periodontitis sites than periodontally healthy sites. For CP patients, a higher prevalence was associated with the seriously involved sites than those moderate and mild implicated sites. H. actinomycetemcomitans is considered to be the one of the periopathogens involved in the etiology of chronic periodontitis.
authors: Key Laboratory of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China.
authors: Shu
authors: S
authors: Meng
authors: Ya-fei
authors: YF
authors: Wu
authors: He
authors: H
authors: Yang
authors: Lei
authors: L
authors: Zhao
authors: Yu-ling
authors: YL
authors: Ou-Yang
mesh_terms: D000328:Adult; D016976:Aggregatibacter actinomycetemcomitans; D055113:Chronic Periodontitis; D004269:DNA, Bacterial; D003773:Dental Plaque; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D008297:Male; D010518:Periodontitis; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S