Phytotoxicity and phytoremediation of 2,6-dinitrotoluene using a model plant, Arabidopsis thaliana.
Biochemical and genetic studies of xenobiotic metabolism in the model plant Arabidopsis have significant potential in providing information for phytoremediation. This paper presents the toxicity of 2,6-dinitrotoluene (2,6-DNT) to Arabidopsis under axenic conditions, the fate and transformation of 2,6-DNT after uptake by the plant, and the effect of a putative glutathione S-transferase (GST), which is highly induced by 2,4,6-trinitrotoluene (TNT) in the previous study, on the detoxification of 2,6-DNT. 2,6-DNT had toxic effects on the growth of Arabidopsis based on whole seedling as well as root growth assays. Using [U- 14C]2,6-DNT, the recovery was over 87% and less than 2% accounted for the mineralization of 2,6-DNT in axenic liquid cultures during the 14d of exposure. About half (48.3%) of the intracellular radioactivity was located in the root tissues in non-sterile hydroponic cultures. 2-Amino-6-nitrotoluene (2A6NT) and two unknown metabolites were produced as transformation products of 2,6-DNT in the liquid media. The metabolites were further characterized by proton NMR spectra and the UV-chromatograms when the plant was fed with either 2,6-DNT or 2A6NT. In addition, polar unknown metabolites were detected at short retention times from radiochromatograms of plant tissue extracts. The GST gene of the wild-type of Arabidopsis in response to 2,6-DNT was induced by 4.7-fold. However, the uptake rates and the tolerance at different concentrations of 2,6-DNT and TNT were not significantly different between the wild-type and the gst mutant indicating that induction of the GST gene is not related to the detoxification of 2,6-DNT.
Yoon
Jong Moon
JM
Department of Chemical and Biological Engineering, Iowa State University, Ames, IA 50011, USA.
Oliver
David J
DJ
Shanks
Jacqueline V
JV
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002250:Carbon Radioisotopes / Q000378:metabolism; D004269:DNA, Bacterial; D004136:Dinitrobenzenes / Q000493:pharmacokinetics*; D018506:Gene Expression Regulation, Plant; D005856:Germ-Free Life; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism*; D008658:Inactivation, Metabolic; D016254:Mutagenesis, Insertional
D002250:Carbon Radioisotopes; D004269:DNA, Bacterial; D004136:Dinitrobenzenes; C036483:T-DNA; D005982:Glutathione Transferase; C023514:2,6-dinitrotoluene