Species-specific primers for Fusarium redolens and a PCR-RFLP technique to distinguish among three clades of Fusarium oxysporum.
The currently available morphological and molecular diagnostic techniques for Fusarium redolens and the three phylogenetic clades of Fusarium oxysporum are problematic. Aligned translation elongation factor 1 alpha (TEF-1 alpha) gene sequences from these species and their close relatives were used to design F. redolens-specific primers, and to identify restriction sites that discriminate among the three clades of F. oxysporum. The F. redolens-specific primers distinguished this species from all others included in the study. There were three TEF-1 alpha-RFLP patterns among formae speciales of F. oxysporum. These PCR-RFLP patterns corresponded with the three clades. These techniques provide simple and inexpensive diagnostic methods for the identification of F. redolens and members of the three clades of F. oxysporum.
Bogale
Mesfin
M
Department of Genetics, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Pretoria, South Africa. mesfin.bogale@fabi.up.ac.za
Wingfield
Brenda D
BD
Wingfield
Michael J
MJ
Steenkamp
Emma T
ET
D016172:DNA Fingerprinting / Q000379:methods; D017931:DNA Primers / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D005656:Fungal Proteins / Q000235:genetics; D005670:Fusarium / Q000145:classification* / Q000235:genetics*; D005838:Genotype; D020648:Peptide Elongation Factor 1 / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity
D017931:DNA Primers; D004271:DNA, Fungal; D005656:Fungal Proteins; D020648:Peptide Elongation Factor 1