Identification of acceptable HLA mismatches in immunized patients using single-antigen-expressing cell lines.
Sera of highly sensitized patients (HSP) contain complex human leukocyte antigen (HLA) antibodies, minimizing the chance to identify crossmatch-negative donors. Expression of 3-6 HLA class I antigens on lymphocytes hampers identification of acceptable mismatches (AMs) by conventional screening (C-SCR). The single-antigen-expressing cell line (SAL) concept circumvents this problem. As a proof of principle, 26 sera of sensitized patients were tested by flow cytometry for immunoglobulin G antibodies against 16 HLA-A and -B SALs. Results were compared with C-SCR. Mostly, SAL reactions confirmed presence/absence of HLA antibodies. While C-SCR sometimes failed to provide unambiguous antibody specificity, we defined 24 new HLA antibody specificities with SALs and proposed 33 new AM by non-reactivity with SALs. Thus, the SAL concept is useful for confirmation/identification of AM and will enhance transplantation of HSP.
Eijsink
C
C
Department of Immunohaematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands. c.eijsink@lumc.nl
Zoet
Y M
YM
Kardol
M J
MJ
Witvliet
M D
MD
Claas
F H J
FH
Mulder
A
A
Doxiadis
I I N
II
D000906:Antibodies / Q000097:blood / Q000276:immunology*; D000915:Antibody Affinity / Q000276:immunology; D000918:Antibody Specificity; D000941:Antigens / Q000096:biosynthesis*; D002460:Cell Line; D003429:Cross Reactions / Q000276:immunology; D006680:HLA Antigens / Q000737:chemistry / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007518:Isoantibodies / Q000737:chemistry; D008214:Lymphocytes / Q000378:metabolism
D000906:Antibodies; D000941:Antigens; D006680:HLA Antigens; D007518:Isoantibodies