The inhibitory effect of Houttuynia cordata extract on stem cell factor-induced HMC-1 cell migration.
Hottuynia cordata Thunb (Saururaceae; HC) is known as a therapeutic drug that has been used in traditional oriental medicine for the treatment of allergy. Mast cells play an important role in a variety of inflammatory diseases, and specifically asthma and atopy. In the present study, we investigated the effect of HC extracts on the migration of the human mast cell line, HMC-1, in response to stem cell factor (SCF). Treatment with HC extracts at a concentration of 10mug/ml for 24h showed no significant decrease in the survival rate of the HMC-1 cells. SCF showed the typical bell-shape curve for the HMC-1 cell chemoattraction with the peak of the curve at the SCF concentration of 100ng/ml. HC-1, which was the whole plant (Houttuynia cordata) extracted with 80% EtOH, and HC-3, which was the residue successively partitioned with EtOAc, both had inhibitory effects on HMC-1 cell movement. After the treatment with 10mug/ml HC-1 extract for 6 and 24h, the chemotactic index (CI) of HMC-1 cells decreased up to 74 and 63%, respectively. HC-3 extract treatment for 6 and 24h lowered the CI to 72 and 44%, respectively. The HC-1 and HC-3 extracts had no inhibitory effect on the mRNA and surface protein expressions of c-kit, SCF receptor. SCF mediated the chemotaxis signaling via NF-kappaB activation, and both extracts inhibited the activation. Therefore, our results indicate that HC-1 and HC-3 extracts decrease the chemotactic ability of HMC-1 cells in response to SCF by inhibiting the NF-kappaB activation, and these substances may be useful for treating mast cell-induced inflammatory diseases.
Kim
In Sik
IS
Department of Biomedical Laboratory Science, School of Medicine, Eulji University, 143-5 Yeuongdu-dong, Jung-gu, Daejeon 301-832, Republic of Korea. orientree@eulji.ac.kr
Kim
Joo-Hwan
JH
Kim
Jin Sook
JS
Yun
Chi-Young
CY
Kim
Dong-Hee
DH
Lee
Ji-Sook
JS
D015153:Blotting, Western; D002460:Cell Line; D002633:Chemotaxis / Q000187:drug effects*; D052859:Houttuynia; D006801:Humans; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D008518:Medicine, East Asian Traditional; D016328:NF-kappa B / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000494:pharmacology; D019009:Proto-Oncogene Proteins c-kit / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019089:Stem Cell Factor / Q000494:pharmacology*
D016328:NF-kappa B; D010936:Plant Extracts; D019089:Stem Cell Factor; D019009:Proto-Oncogene Proteins c-kit