title: Comparison of proteomes in various human plasma preparations by two-dimensional gel electrophoresis.
abstract: Serum or plasma can be utilized in a variety of studies targeted toward the discovery of disease biomarkers. In this study, the proteome profiles of plasma samples prepared using various anticoagulants (EDTA, heparin or citrate), were compared with those of serum using two-dimensional electrophoresis (2-DE). Proteins which evidenced different levels in the plasma and serum were screened and identified using ESI-Q-TOF MS/MS. The proteins which became detectable after the removal of fibrinogen from serum were identified as pigment epithelial differentiating factor (four spots), fetuin-like protein, and the hemopexin precursor. In particular, three proteins, pre-serum amyloid P component, plasma glutathione peroxidase precursor, and tetranectin, evidenced increased volume intensity only in the plasma samples prepared with EDTA.
authors: School of Life Sciences and Biotechnology, Korea University, 1, 5-ka, Anam-dong, Sungbuk-ku, Seoul 136-701, Republic of Korea.
authors: Hyun-Jung
authors: HJ
authors: Kim
authors: Mi-Ryung
authors: MR
authors: Kim
authors: Eun-Jung
authors: EJ
authors: So
authors: Chan-Wha
authors: CW
authors: Kim
mesh_terms: D000328:Adult; D001798:Blood Proteins / Q000235:genetics* / Q000302:isolation & purification*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000302:isolation & purification; D007202:Indicators and Reagents; D007525:Isoelectric Focusing / Q000379:methods; D008297:Male; D010949:Plasma; D020543:Proteome; D044967:Serum; D012709:Serum Albumin / Q000302:isolation & purification
chemical_list: D001798:Blood Proteins; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D020543:Proteome; D012709:Serum Albumin