Costimulatory ligand CD70 is delivered to the immunological synapse by shared intracellular trafficking with MHC class II molecules.
TNF family member CD70 is the ligand of CD27, a costimulatory receptor that shapes effector and memory T cell pools. Tight control of CD70 expression is required to prevent lethal immunodeficiency. By selective transcription, CD70 is largely confined to activated lymphocytes and dendritic cells (DC). We show here that, in addition, specific intracellular routing controls its plasma membrane deposition. In professional antigen-presenting cells, such as DC, CD70 is sorted to late endocytic vesicles, defined as MHC class II compartments (MIIC). In cells lacking the machinery for antigen presentation by MHC class II, CD70 travels by default to the plasma membrane. Introduction of class II transactivator sufficed to reroute CD70 to MIIC. Vesicular trafficking of CD70 and MHC class II is coordinately regulated by the microtubule-associated dynein motor complex. We show that when maturing DC make contact with T cells in a cognate fashion, newly synthesized CD70 is specifically delivered via MIIC to the immunological synapse. Therefore, we propose that routing of CD70 to MIIC serves to coordinate delivery of the T cell costimulatory signal in time and space with antigen recognition.
Keller
Anna M
AM
Division of Immunology and Tumor Biology, The Netherlands Cancer Institute, 1066 CX, Amsterdam, The Netherlands.
Groothuis
Tom A
TA
Veraar
Elise A M
EA
Marsman
Marije
M
Maillette de Buy Wenniger
Lucas
L
Janssen
Hans
H
Neefjes
Jacques
J
Borst
Jannie
J
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology* / Q000648:ultrastructure; D001854:Bone Marrow Cells / Q000166:cytology; D053282:CD27 Ligand / Q000276:immunology* / Q000648:ultrastructure; D002462:Cell Membrane / Q000276:immunology / Q000648:ultrastructure; D002478:Cells, Cultured; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000276:immunology* / Q000648:ultrastructure; D019084:Fluorescent Antibody Technique, Indirect; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006367:HeLa Cells; D000949:Histocompatibility Antigens Class II / Q000276:immunology* / Q000648:ultrastructure; D006801:Humans; D008024:Ligands; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000276:immunology; D008545:Melanoma / Q000276:immunology / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence
D053282:CD27 Ligand; D000949:Histocompatibility Antigens Class II; D008024:Ligands; D008070:Lipopolysaccharides