Combined chemical and hypoxic protection against whole-body X-irradiation in the nestling rat.
Litters of 8- to 10-day-old nestling rats were protected against whole-body x-irradiation by cysteamine, AET, and deep hypothermia, singly, and by a combination of cysteamine pretreatment and deep hypothermia. The different treatments were randomized among littermates to minimize animal variation. A comparison of the LD50(30) values obtained for the different treatments gave a dose-reduction factor of 3.3 for the combined treatment, which was significantly greater than the factor for hypothermia alone, 2.6, or that for cysteamine alone, 1.4. Cysteamine therefore provided additional protection in the fully hypoxic (hypothermic) animal. Judged from the histological appearance of the gonads of the surviving male nestling rats, killed 30 days after irradiation, hypothermia afforded some protection to the germinal epithelium. Cysteamine, although injected when the testes were abdominal, had no demonstrable protective effect.
Shewell
J
J
Department of Pathology, St. Mary's Hospital Medical School, London, England.
D000818:Animals; D003543:Cysteamine / Q000008:administration & dosage*; D005260:Female; D007036:Hypothermia, Induced / Q000379:methods*; D000860:Hypoxia / Q000517:prevention & control; D008297:Male; D011832:Radiation Injuries / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar; D013534:Survival; D015996:Survival Rate; D016896:Treatment Outcome; D014916:Whole-Body Irradiation / Q000009:adverse effects*; D014965:X-Rays / Q000009:adverse effects; D000338:beta-Aminoethyl Isothiourea / Q000008:administration & dosage*
D011837:Radiation-Protective Agents; D000338:beta-Aminoethyl Isothiourea; D003543:Cysteamine