title: [Expression of p53 gene with DNA polbeta and CMV promoter in salivary adenoid cystic carcinoma cells].
abstract: OBJECTIVE
  To evaluate the activity of DNA polbeta promoter on p53 gene in salivary adenoid cystic carcinoma (SACC) cells.
  
  
  METHODS
  The luciferase activity was examined and used to evaluate the activity of DNA polbeta promoter on SACC-83 cells. Eukaryotic expression plasmids of p53 gene were constructed and stably transfected into SACC-83 cells. RT-PCR was used to assess the expression of p53 gene. The SACC-83 cells were subjected to the treatments of H2O2, ultraviolet radiation, Bleocin, and affected p53 mRNA and protein level in SACC-83 cells were characterized with RT-PCR and Western blotting.
  
  
  RESULTS
  The result of luciferase activity proved that the activity of DNA polbeta promoter in SACC-83 cells was much higher than that of CMV promoter. The results of RT-PCR suggested that p53 gene with different promoters were all expressed effectively, but the expression efficiency was different. It was greater in DNA polbeta group than in CMV group. After DNA damage, p53 gene expression increased and DNA polbeta promoter could enhance the expression of p53 gene more than CMV promoter. The results of Western blotting indicated that the expression of P53 protein between the two groups did not show any difference.
  
  
  CONCLUSIONS
  In SACC cells, the activity of DNA polbeta promoter was increased and DNA polbeta promoter could enhance the expression of p53.
authors: Dept. of Oral Pathology, College of Stomatology, Hebei Medical University, Shijiazhuang 050017, China.
authors: Bing-zhi
authors: BZ
authors: Yan
authors: Jie
authors: J
authors: Wang
authors: Bo
authors: B
authors: Zhang
authors: Fu-sheng
authors: FS
authors: Dong
authors: Lin
authors: L
authors: Hou
authors: Xu
authors: X
authors: Wang
mesh_terms: D003528:Carcinoma, Adenoid Cystic; D045744:Cell Line, Tumor; D004247:DNA; D016158:Genes, p53; D006801:Humans; D006861:Hydrogen Peroxide; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger; D012468:Salivary Gland Neoplasms; D014162:Transfection; D014466:Ultraviolet Rays
chemical_list: D012333:RNA, Messenger; D004247:DNA; D006861:Hydrogen Peroxide