title: A fundamental regulatory role of formate on thuringiensin production by resting cell of Bacillus thuringiensis YBT-032.
abstract: In this work, a fundamental regulatory role of formate on thuringiensin production by resting cell of Bacillus thuringiensis YBT-032 was investigated. Nicotinamide adenine dinucleotide (NADH) production and formate dehydrogenase activity increased with formate addition from 0.5 to 2.0 g/L, respectively. However, with the formate addition of 1.5 g/L, the activities of pyruvate kinase and glucose 6-phosphate dehydrogenase reached a peak and increased by 316 and 150% relative to those of the control, respectively. In addition, intracellular production of pyruvate, aspartate, citrate and adenine were significantly enhanced by 75, 66, 32 and 78% as well. An improvement (90%) of thuringiensin production was also successfully obtained. Interestingly to point out, thuringiensin yield was closely correlative with adenine production, and the linear relationship was also observed. The results suggest that appropriate formate addition did act as a modulator and facilitate carbon flux in glycolysis and pentose phosphate pathway to synthesize adenine and thuringiensin via intracellular NADH availability.
authors: National Engineering Research Center for Microbial Pesticides, State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors: Wang
authors: W
authors: Zhi
authors: Chen
authors: C
authors: Shouwen
authors: Ruan
authors: R
authors: Lifang
authors: Sun
authors: S
authors: Ming
authors: Yu
authors: Y
authors: Ziniu
mesh_terms: D000225:Adenine / Q000096:biosynthesis*; D000241:Adenosine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D001413:Bacillus thuringiensis / Q000187:drug effects / Q000378:metabolism*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005561:Formates / Q000008:administration & dosage*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects / Q000502:physiology*; D006019:Glycolysis / Q000187:drug effects; D010427:Pentose Phosphate Pathway / Q000187:drug effects / Q000502:physiology; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D013400:Sugar Acids
chemical_list: D005561:Formates; D013400:Sugar Acids; C030544:formic acid; D000225:Adenine; C068295:thuringiensin; D000241:Adenosine