Molecular characterization of imipenem-resistant Pseudomonas aeruginosa in Hiroshima, Japan.
Pseudomonas aeruginosa showing resistance to imipenem were found in 100 of 1,058 strains (9.5%) from six hospitals (a-f) in Hiroshima City, Japan. Of the 100 strains, 14 (14%) were double disk synergy test positive using sodium mercaptoacetic acid disks, and 18 (18%) were bla(IMP-1) or bla(VIM-2) allele positive by polymerase chain reaction (PCR). Among 100 imipenem-resistant strains, 32 were categorized into multi-drug resistant strains, in which 13 were positive for the metallo-beta-lactamase gene. Fifty-one strains (51%) among the 100 imipenem-resistant strains had elevated RND efflux pump activity against levofloxacin. But only 6 of 51 strains were classified as multi-drug resistant strains. The pulsed field gel electrophoresis analysis of the Spe I-digested DNA from the 100 isolates suggested not only clonal spread but spread of heterogeneous clones started to contribute to the prevalence of metallo-beta-lactamase producing P. aeruginosa strains in Japanese hospitals.
Ohara
Masaru
M
Department of Bacteriology, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima, Japan.
Kouda
Shuntaro
S
Onodera
Makoto
M
Fujiue
Yoshihiro
Y
Sasaki
Megumi
M
Kohara
Tadahiro
T
Kashiyama
Seiya
S
Hayashida
Shizue
S
Kadono
Manami
M
Komatsuzawa
Hitoshi
H
Gotoh
Naomasa
N
Usui
Tuguru
T
Itaha
Hideyuki
H
Kuwabara
Masao
M
Yokoyama
Takashi
T
Sugai
Motoyuki
M
D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003428:Cross Infection / Q000382:microbiology; D024901:Drug Resistance, Multiple, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D006801:Humans; D015378:Imipenem / Q000494:pharmacology*; D007564:Japan; D008297:Male; D005810:Multigene Family; D010802:Phylogeny; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics
D000900:Anti-Bacterial Agents; D015378:Imipenem; D001618:beta-Lactamases