Oxidative stress and delta-ALA-D activity in chronic renal failure patients.
In this paper, we studied the influence of uremia and hemodialysis on oxidative parameters and delta-aminolevulinic acid dehydratase (delta-ALA-D) activity in control subjects, patients with chronic renal failure (CRF) on hemodialysis treatment (HD) and in patients not undergoing hemodialysis (ND). An increased lipid peroxidation was observed in the serum of HD and ND patients, as measured by the MDA serum levels. However, the level of MDA from erythrocytes was only elevated in HD patients. Blood catalase activity was increased in HD and ND groups. This study also showed a decreased activity of blood delta-aminolevulinic acid dehydratase (delta-ALA-D) in both groups of patients. This study demonstrated a positive correlation between ALA-D activity and hemoglobin, suggesting that inhibition of this enzyme might enhance anemia in CRF. A negative correlation was found between the alteration in delta-ALA-D activity and oxidative stress, which may indicate that the inhibition of ALA-D can be used as an index of oxidative stress.
da Silva
Adriane C
AC
Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Av. Roraima, 97105-900 Santa Maria, RS, Brazil.
Rocha
João B T
JB
Morsch
André L B
AL
Zanin
Rafael F
RF
Kaizer
Rosilene
R
Maldonado
Paula Acosta
PA
Arantes
Luís C
LC
Silva
Luís A
LA
Morsch
Vera M
VM
Schetinger
Maria R C
MR
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002374:Catalase / Q000097:blood / Q000378:metabolism; D005260:Female; D006454:Hemoglobins; D006801:Humans; D007676:Kidney Failure, Chronic / Q000503:physiopathology*; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D000623:Porphobilinogen Synthase / Q000097:blood / Q000378:metabolism*; D006435:Renal Dialysis; D014511:Uremia / Q000503:physiopathology*
D006454:Hemoglobins; D002374:Catalase; D000623:Porphobilinogen Synthase