Response of cells on surface-induced nanopatterns: fibroblasts and mesenchymal progenitor cells.
Ultrathin films of a poly(styrene)-block-poly(2-vinylpyrindine) diblock copolymer (PS-b-P2VP) and poly(styrene)-block-poly(4-vinylpyrindine) diblock copolymer (PS-b-P4VP) were used to form surface-induced nanopattern (SINPAT) on mica. Surface interaction controlled microphase separation led to the formation of chemically heterogeneous surface nanopatterns on dry ultrathin films. Two distinct nanopatterned surfaces, namely, wormlike and dotlike patterns, were used to investigate the influence of topography in the nanometer range on cell adhesion, proliferation, and migration. Atomic force microscopy was used to confirm that SINPAT was stable under cell culture conditions. Fibroblasts and mesenchymal progenitor cells were cultured on the nanopatterned surfaces. Phase contrast and confocal laser microscopy showed that fibroblasts and mesenchymal progenitor cells preferred the densely spaced wormlike patterns. Atomic force microscopy showed that the cells remodelled the extracellular matrix differently as they migrate over the two distinctly different nanopatterns.
Khor
Hwei Ling
HL
Max-Planck Institute for Polymer Research, 10 Ackermannweg, 55128 Mainz, Germany. khor@mpip-mainz.mpg.de
Kuan
Yujun
Y
Kukula
Hildegard
H
Tamada
Kaoru
K
Knoll
Wolfgang
W
Moeller
Martin
M
Hutmacher
Dietmar W
DW
D018929:Cell Culture Techniques; D002465:Cell Movement; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000502:physiology; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry*; D011137:Polystyrenes; D011145:Polyvinyls; D013499:Surface Properties
D011137:Polystyrenes; D011145:Polyvinyls; C521881:poly(styrene)-block-poly(2-vinylpyrindine) diblock copolymer; C521882:poly(styrene)-block-poly(4-vinylpyrindine) diblock copolymer