Determination of calycosin-7-O-beta-D-glucopyranoside in rat plasma and urine by HPLC.
A reversed-phase high-performance liquid chromatographic (HPLC) assay for calycosin-7-O-beta-D-glucopyranoside in rat plasma and urine with solid-phase extraction (SPE) was developed. Rutin was employed as an internal standard. The mobile phase consisted of acetonitrile-water (16:84, v/v) at a flow rate of 1.0 mL/min. Detection was set at 280 nm. The limit of quantitation of calycosin-7-O-beta-D-glucopyranoside was 0.2 microg/mL in both plasma and urine. The standard curve was linear from 0.2 to 10.0 microg/mL in plasma, and 0.2 to 5.0 microg/mL in urine. Both intra- and inter-day precision of the calycosin-7-O-beta-d-glucopyranoside were determined and their RSD did not exceed 10%. The method was successfully applied to the analysis of samples obtained from a basic pharmacokinetic study, in which calycosin-7-O-beta-d-glucopyranoside was administered orally to rats.
Ye
Guan
G
Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China.
Ma
Rong-Rong
RR
Li
Zhi-Xiong
ZX
Wang
Hui
H
Zhu
Hai-Yan
HY
Sun
Zhao-Lin
ZL
Huang
Cheng-Gang
CG
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005960:Glucosides / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D007529:Isoflavones / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D012680:Sensitivity and Specificity
D005960:Glucosides; D007529:Isoflavones; C488311:calycosin-7-O-glucopyranoside