title: Effects of vanadium(V) and/or chromium(III) on L-ascorbic acid and glutathione as well as iron, zinc, and copper levels in rat liver and kidney.
abstract: This study investigated the selected parameters of the antioxidant system in liver and kidney after in vivo administration of vanadium and/or chromium in rats. Outbred 2-mo-old albino male Wistar rats received drinking water for 12 wk with either sodium metavanadate (SMV; group II); chromium chloride (Cr; group III); or sodium metavanadate and chromium chloride (SMV-Cr; group IV); and group I (control) received deionized water. Chronic treatment with V alone or in combination with Cr produced a significant increase in kidney relative weight. Further, giving rats V alone also led to a significant elevation in liver relative weight. An increase in hepatic Fe concentration and renal Zn content occurred after treatment with V or Cr, respectively. The rats coadministered V and Cr had significantly higher levels of Fe in liver and Zn in kidneys. Simultaneous administration of these two elements resulted in a significant decrease in renal L-ascorbic acid concentration. V given alone significantly decreased GSH content and GSH/GSSG ratio in liver and kidney as well as increased GSSG concentration in liver, whereas Cr alone produced a significant decrease in GSH content in kidney and GSH/GSSG ratio in both organs. In the SMV-Cr-treated group a significant decrease in renal GSH concentration and GSH/GSSG ratio in both organs occurred. A significant increase in liver GSSG content was also found. The observed significant changes in kidney GSH content and in GSH/GSSG ratio in both rat tissues after Cr might result from the pro-oxidant actions of this metal. Thus, oxidative stress, which is a major pathway for V-induced toxicity, might also be associated with Cr(III)-induced adverse effects in rats.
authors: Department of Cell Biology, Institute of Environmental Protection, John Paul II Catholic University of Lublin, Lublin, Poland. cellbiol@kul.lublin.pl
authors: Agnieszka
authors: A
authors: Scibior
authors: Halina
authors: H
authors: Zaporowska
mesh_terms: D000284:Administration, Oral; D000818:Animals; D021461:Animals, Outbred Strains; D001205:Ascorbic Acid / Q000378:metabolism*; D002857:Chromium / Q000032:analysis / Q000633:toxicity*; D003300:Copper / Q000032:analysis; D004359:Drug Therapy, Combination; D005978:Glutathione / Q000378:metabolism*; D019803:Glutathione Disulfide / Q000378:metabolism; D007501:Iron / Q000032:analysis; D007668:Kidney / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D008099:Liver / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D019216:Metals, Heavy / Q000032:analysis*; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D014639:Vanadium / Q000032:analysis / Q000633:toxicity*; D014881:Water Supply; D015032:Zinc / Q000032:analysis
chemical_list: D019216:Metals, Heavy; D014639:Vanadium; D002857:Chromium; D003300:Copper; D007501:Iron; D005978:Glutathione; D015032:Zinc; D001205:Ascorbic Acid; D019803:Glutathione Disulfide