Interaction of water activity and bicarbonate salts in the inhibition of growth and mycotoxin production by Fusarium and Aspergillus species of importance to corn.
The combined effects of water activity (a(w)) and ammonium/sodium bicarbonate on growth and mycotoxin production in corn by Fusarium and Aspergillus species were investigated. Interaction was observed between the salts and a(w) on the colony growth rates and lag phase durations of all isolates. Growth stimulation at low salt levels was observed only for the Fusarium isolates as the fastest growth of F. verticillioides and F. proliferatum occurred at levels of 0.1-0.2 and 0.5% ammonium and sodium bicarbonate, respectively. Although the complete inhibition of the growth of the Fusarium and Aspergillus isolates investigated took place at a level of 1% ammonium bicarbonate as much as 4% sodium bicarbonate failed to completely inhibit the growth of the Aspergillus isolates. Increase in concentration of either salt generally resulted in large reductions of both fumonisin B(1) and aflatoxin B(1) production. According to the sensorial analysis performed, corn treated with up to 1% ammonium bicarbonate was still acceptable for consumption, whereas corn treated with at least 2% sodium bicarbonate was determined to be sensorially unsuitable. Ammonium bicarbonate can be concluded to be more suitable for protecting stored corn from fungal contamination as it was capable of completely inhibiting both growth and mycotoxin production of the Fusarium and Aspergillus isolates of most importance to corn at levels that were still sensorially acceptable. Therefore ammonium bicarbonate could possibly be applied as a cheap and easy to apply treatment for use in resource limited developing countries.
Samapundo
S
S
Ghent University, Faculty of Bioscience Engineering, Department of Food Safety and Food Quality, Laboratory of Food Microbiology and Food Preservation, Coupure Links 653, Gent, Belgium.
Devlieghere
F
F
De Meulenaer
B
B
Lamboni
Y
Y
Osei-Nimoh
D
D
Debevere
J M
JM
D001230:Aspergillus / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D001639:Bicarbonates / Q000494:pharmacology*; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D004305:Dose-Response Relationship, Drug; D005506:Food Contamination / Q000032:analysis / Q000517:prevention & control; D005519:Food Preservation / Q000379:methods*; D037341:Fumonisins / Q000032:analysis / Q000378:metabolism; D005670:Fusarium / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D007700:Kinetics; D009183:Mycotoxins / Q000032:analysis / Q000096:biosynthesis*; D017693:Sodium Bicarbonate / Q000494:pharmacology*; D013045:Species Specificity; D014867:Water / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry / Q000382:microbiology*
D001639:Bicarbonates; D037341:Fumonisins; D009183:Mycotoxins; D014867:Water; C027043:ammonium bicarbonate; D017693:Sodium Bicarbonate