Immunomodulation of Labeo rohita juveniles due to dietary gelatinized and non-gelatinized starch.
A 60 days experiment was conducted to study the effect of dietary gelatinized (G) and non-gelatinized (NG) starch on immunomodulation of Labeo rohita juveniles. Two hundred and thirty four juveniles (av. wt. 2.53+/-0.04) were randomly distributed in six treatment groups with each of three replicates. Six semi-purified diets containing NG and G corn starch, each at six levels of inclusion (0, 20, 40, 60, 80, 100) were prepared viz., T(1) (100% NG, 0% G starch), T(2) (80% NG, 20% G starch), T(3) (60% NG, 40% G starch), T(4) (40% NG, 60% G starch), T(5) (20% NG, 80% G starch) and T(6) (0% NG, 100% G starch). After a feeding period of 60 days, the juveniles were challenged with Aeromonas hydrophila to study their immunomodulation due to feeding of G and NG starch. RBC and haemoglobin content were significantly (P<0.05) reduced due to bacterial challenge, but dietary starch (G/NG starch) had no effect on it. G:NG starch ratio in the feed had significant effect on total leukocyte count during pre- and post-challenge periods. The leukocyte count concomitantly increased with the increased level of G starch in the diet. Highest albumin/globulin (A/G) ratio was recorded in T6 group (100% G starch) and lowest in T1 group (100% NG starch) group followed by T2 group both in pre- and post-challenge periods. NBT, lysozyme activity, total protein and globulin content were highest in T2 group (80% NG, 20% G starch) both in pre- and post-challenge periods. After challenge with A. hydrophila, the highest survival was recorded in T2 group, whereas lowest survival was recorded in T6 group. Conclusively high level of G starch was found to be immunosuppressive in Labeo rohita juveniles and NG:G starch ratio of 80:20 seems to be optimum for promoting growth and protecting immunity in L. rohita juveniles.
Kumar
Vikas
V
Department of Fish Nutrition and Biochemistry, Central Institute of Fisheries Education, Seven Bungalow, Versova, Andheri (W), Mumbai 400061, Maharashtra, India. vikaskr_2005@rediffmail.com
Sahu
N P
NP
Pal
A K
AK
Kumar
Shivendra
S
D016980:Aeromonas hydrophila / Q000276:immunology; D000818:Animals; D001772:Blood Cell Count / Q000662:veterinary; D001798:Blood Proteins / Q000378:metabolism; D001835:Body Weight / Q000276:immunology; D002347:Carps / Q000097:blood / Q000276:immunology*; D002784:Cholesterol / Q000097:blood; D004040:Dietary Carbohydrates / Q000008:administration & dosage / Q000276:immunology*; D005393:Fish Diseases / Q000276:immunology / Q000382:microbiology; D005780:Gelatin / Q000008:administration & dosage / Q000276:immunology; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary; D006454:Hemoglobins / Q000378:metabolism; D007155:Immunologic Factors / Q000276:immunology*; D009113:Muramidase / Q000097:blood; D011897:Random Allocation; D016897:Respiratory Burst / Q000276:immunology; D013213:Starch / Q000008:administration & dosage / Q000276:immunology*; D016019:Survival Analysis; D014280:Triglycerides / Q000097:blood
D001798:Blood Proteins; D004040:Dietary Carbohydrates; D006454:Hemoglobins; D007155:Immunologic Factors; D014280:Triglycerides; D005780:Gelatin; D013213:Starch; D002784:Cholesterol; D009113:Muramidase