title: 15N metabolic labeling of mammalian tissue with slow protein turnover.
abstract: We previously reported the metabolic 15N labeling of a rat where enrichment ranged from 94% to 74%. We report here an improved labeling strategy which generates 94% 15N enrichment throughout all tissues of the rat. A high 15N enrichment of the internal standard is necessary for accurate quantitation, and thus, this approach will allow quantitative mass spectrometry analysis of animal models of disease targeting any tissue.
authors: Department of Cell Biology, 10550 North Torrey Pines Road, SR11, The Scripps Research Institute, LaJolla, California 92037, USA.
authors: Daniel B
authors: DB
authors: McClatchy
authors: Meng-Qiu
authors: MQ
authors: Dong
authors: Christine C
authors: CC
authors: Wu
authors: John D
authors: JD
authors: Venable
authors: John R
authors: JR
authors: Yates
mesh_terms: D000818:Animals; D004032:Diet; D005260:Female; D009587:Nitrogen Isotopes / Q000737:chemistry / Q000378:metabolism*; D011247:Pregnancy; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D009587:Nitrogen Isotopes; D011506:Proteins