title: Proteomic analysis of Bacillus cereus growing in liquid soil organic matter.
abstract: Bacillus cereus is believed to be a soil bacterium, but studied solely in laboratory culture media. The aim of this study was to assess the physiology of B. cereus growing on soil organic matter by a proteomic approach. Cells were cultured to mid-exponential phase in soil extracted solubilized organic matter (SESOM), which mimics the nutrient composition of soil, and in Luria-Bertani broth as control. Silver staining of the two-dimensional gels revealed 234 proteins spots up-regulated when cells were growing in SESOM, with 201 protein spots down-regulated. Forty-three of these differentially expressed proteins were detected by Colloidal Coomassie staining and identified by matrix assisted laser desorption ionization-time of flight MS of tryptic digests. These differentially expressed proteins covered a range of functions, primarily amino acid, lipid, carbohydrate and nucleic acid metabolism. These results suggested growth on soil-associated carbohydrates, fatty acids and/or amino acids, concomitant with shifts in cellular structure.
authors: Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57007, USA.
authors: Yun
authors: Y
authors: Luo
authors: Sébastien
authors: S
authors: Vilain
authors: Birgit
authors: B
authors: Voigt
authors: Dirk
authors: D
authors: Albrecht
authors: Michael
authors: M
authors: Hecker
authors: Volker S
authors: VS
authors: Brözel
mesh_terms: D001409:Bacillus cereus / Q000737:chemistry* / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D001426:Bacterial Proteins / Q000032:analysis*; D003470:Culture Media / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D015964:Gene Expression Regulation, Bacterial; D053858:Metabolic Networks and Pathways / Q000235:genetics; D020543:Proteome / Q000032:analysis*; D029963:Quercus / Q000382:microbiology; D012394:Rosaniline Dyes; D016622:Silver Staining; D012988:Soil Microbiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014197:Trees / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D020543:Proteome; D012394:Rosaniline Dyes; C004692:coomassie Brilliant Blue