title: Coexpression of CD1a, langerin and Birbeck's granules in Langerhans cell histiocytoses (LCH) in children: ultrastructural and immunocytochemical studies.
abstract: Langerhans cell histiocytoses (LCH) represent rare diseases of unclear etiology and pathogenesis. Most of the cases include children, 1 to 15 years of age, and various organs are involved (bones, skin, liver, lymph nodes, bone marrow and other). The diagnosis of LCH used to be established by biopsy of the inflamed tissue and demonstration of expression of markers specific for Langerhans cells: CD1a and langerin. The diagnosis can be ultimately confirmed by demonstration of Birbeck's granules in the electron microscopy. The present study was aimed at immunocytochemical demonstration, in the examined LCH material (skin, bones, lymph nodes), of the specific antigen expression and at comparing it with the presence of Birbeck's granules. In the examined 11 cases co-expression of CD1a with langerin and with the presence of Birbeck's granules was noted. Also in all examined biopsies the expression of S-100 protein on inflammatory cells was found. The results corroborate the usefulness of immunocytochemical studies on CD 1 a and langerin expression in diagnosis of LCH.
authors: Department of Histology and Embryology, University School of Medicine, Wrocław, Poland. . piotr@hist.am.wroc.pl
authors: Piotr
authors: P
authors: Dziegiel
authors: Barbara
authors: B
authors: Dolilńska-Krajewska
authors: Malgorzata
authors: M
authors: Dumańska
authors: Jadwiga
authors: J
authors: Wecławek
authors: Michał
authors: M
authors: Jeleń
authors: Marzena
authors: M
authors: Podhorska-Okołów
authors: Ewa
authors: E
authors: Jagoda
authors: Magdalena
authors: M
authors: Fic
authors: Maciej
authors: M
authors: Zabel
mesh_terms: D000293:Adolescent; D015703:Antigens, CD / Q000378:metabolism*; D018949:Antigens, CD1 / Q000276:immunology / Q000378:metabolism*; D002675:Child, Preschool; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D005260:Female; D006646:Histiocytosis, Langerhans-Cell / Q000175:diagnosis* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D007231:Infant, Newborn; D007801:Langerhans Cells / Q000276:immunology* / Q000648:ultrastructure; D037181:Lectins, C-Type / Q000378:metabolism*; D008297:Male; D037241:Mannose-Binding Lectins / Q000378:metabolism*
chemical_list: D015703:Antigens, CD; D018949:Antigens, CD1; C419393:CD1a antigen; C121916:CD207 protein, human; D037181:Lectins, C-Type; D037241:Mannose-Binding Lectins