Cloning, sequencing of bone morphogenetic protein from sea urchin, Hemicentrotus pulcherrimus.
A cDNA coding for bone morphogenetic protein (BMP) homolog of the sea urchin, Hemicentrotus pulcherrimus, was isolated from mid-gastrula using reverse transcription-polymerase chain reaction (RT-PCR) technique. The 2314 nucleotide sequence contains a 1383 open reading frame corresponding to a translation product of 461 amino acids. Comparison of the nucleotide and deduced amino acid sequence with BMP isolated from Strongylocentrotus purpuratus (SpBMP5-7; accession No. Z48313) shows a high degree of conservation. HpBMP seems to belong to the 60A subgroup as a result. A mRNA coding H. pulcherrimus BMP (HpBMP) was not detected in the unfertilized egg, but it was detected from blastula to prism stages.
Kurogi
Yumi
Y
Department of Chemistry and Bioscience, Faculty of Science, Kagoshima University, Korimoto, Kagoshima 890-0065, Japan.
Ohta
Kazumasa
K
Nakazawa
Tohru
T
Tosuji
Hiroaki
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019485:Bone Morphogenetic Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary / Q000235:genetics; D047330:Hemicentrotus / Q000196:embryology / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid
D019485:Bone Morphogenetic Proteins; D018076:DNA, Complementary