A robotic synthesis of [18F]fluoromisonidazole ([18F]FMISO).
This study aimed to develop an automated synthesis of [18F]fluoromisonidazole ([18F]FMISO) using a Scanditronix Anatech RB III robotic system. [18F]HF was produced via the 18O(p,n)18F reaction using a Scanditronix MC17F cyclotron. On average, a typical run produced [18F]FMISO with an uncorrected radiochemical yield of 30+/-5% at end of synthesis (EOS) from the irradiation of 95% enriched [18O]water. The total synthesis time was 65 min. The retention time of [18F]FMISO (the radio-peak) was 4.9 min, which was consistent with the authentic FMISO (the ultraviolet peak). The radiochemical purity was greater than 97%. Preparation of [18F]FMISO using the automated robotic system is highly reliable and reproducible, and the radiation burden for the operator can be largely reduced. Sufficient radioactivities of [18F]FMISO could be obtained for non-invasive tumor hypoxia imaging in vivo with positron emission tomography (PET).
Chang
C W
CW
Department of Biomedical Imaging and Radiological Sciences, National Yang-Ming University, No. 155, Sec. 2, Li-Nong Street, Taipei 112, Taiwan.
Chou
T K
TK
Liu
R S
RS
Wang
S J
SJ
Lin
W J
WJ
Chen
C H
CH
Wang
H E
HE
D005462:Fluorine Radioisotopes / Q000737:chemistry*; D000860:Hypoxia / Q000000981:diagnostic imaging; D008920:Misonidazole / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D009369:Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism; D049268:Positron-Emission Tomography; D019275:Radiopharmaceuticals / Q000138:chemical synthesis*; D012371:Robotics
D005462:Fluorine Radioisotopes; D019275:Radiopharmaceuticals; C031843:fluoromisonidazole; D008920:Misonidazole