Polymeric nanoparticles for protein kinase activity.
Nanoparticles were prepared from poly-ion complexes, possessing both PEI-FITC-(PKA-specific substrate) (kemptide) and PAA-TRITC, which produce intermolecular FRET; the nanoparticles were dissociated by phosphorylation, presented a strong FITC intensity and can be applied to high-throughput screening for large chemical libraries, for drug discovery of kinase inhibitors.
Kim
Jong-Ho
JH
Biomedical Research Center, Korea Institute of Science and Technology, 39-1 Hawolgok-dong, Seongbuk-gu, Seoul 136-791, South Korea.
Lee
Seulki
S
Kim
Kwangmeyung
K
Jeon
Hyesung
H
Park
Rang-Woon
RW
Kim
In-San
IS
Choi
Kuiwon
K
Kwon
Ick Chan
IC
D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*
D011108:Polymers; D017868:Cyclic AMP-Dependent Protein Kinases