Multiple high-throughput analyses monitor the response of E. coli to perturbations.
Analysis of cellular components at multiple levels of biological information can provide valuable functional insights. We performed multiple high-throughput measurements to study the response of Escherichia coli cells to genetic and environmental perturbations. Analysis of metabolic enzyme gene disruptants revealed unexpectedly small changes in messenger RNA and proteins for most disruptants. Overall, metabolite levels were also stable, reflecting the rerouting of fluxes in the metabolic network. In contrast, E. coli actively regulated enzyme levels to maintain a stable metabolic state in response to changes in growth rate. E. coli thus seems to use complementary strategies that result in a metabolic network robust against perturbations.
Ishii
Nobuyoshi
N
Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
Nakahigashi
Kenji
K
Baba
Tomoya
T
Robert
Martin
M
Soga
Tomoyoshi
T
Kanai
Akio
A
Hirasawa
Takashi
T
Naba
Miki
M
Hirai
Kenta
K
Hoque
Aminul
A
Ho
Pei Yee
PY
Kakazu
Yuji
Y
Sugawara
Kaori
K
Igarashi
Saori
S
Harada
Satoshi
S
Masuda
Takeshi
T
Sugiyama
Naoyuki
N
Togashi
Takashi
T
Hasegawa
Miki
M
Takai
Yuki
Y
Yugi
Katsuyuki
K
Arakawa
Kazuharu
K
Iwata
Nayuta
N
Toya
Yoshihiro
Y
Nakayama
Yoichi
Y
Nishioka
Takaaki
T
Shimizu
Kazuyuki
K
Mori
Hirotada
H
Tomita
Masaru
M
D002853:Chromatography, Liquid; D019295:Computational Biology; D019075:Electrophoresis, Capillary; D015180:Electrophoresis, Gel, Two-Dimensional; D004790:Enzyme Induction; D004794:Enzyme Repression; D004798:Enzymes / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D005798:Genes, Bacterial; D013058:Mass Spectrometry; D053858:Metabolic Networks and Pathways / Q000235:genetics; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D020543:Proteome; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D049490:Systems Biology / Q000379:methods*; D014158:Transcription, Genetic
D004798:Enzymes; D029968:Escherichia coli Proteins; D020543:Proteome; D012333:RNA, Messenger