title: 5-Methoxy-N,N-diisopropyltryptamine (Foxy), a selective and high affinity inhibitor of serotonin transporter.
abstract: 5-Methoxy-N,N-diisopropyltryptamine (5-MeO-DIPT) is a synthetic orally active hallucinogenic tryptamine derivative, known also as Foxy or Foxy methoxy. However, few studies have examined its effects in vitro. In the present study, we investigated the actions of 5-MeO-DIPT against monoamine neurotransmitter transporters, including the transporters for dopamine (DAT), norepinephrine (NET), and serotonin (SERT), using COS-7 cells heterologously expressing these transporters and rat brain synaptosomes. 5-MeO-DIPT specifically inhibited the uptake of [3H]serotonin (5-HT) by the SERT-expressing COS-7 cells and rat striatal synaptosomes in a high affinity manner at concentrations similar to those for cocaine. The effect was reversible and competitive. 5-MeO-DIPT failed to stimulate reverse transport of [3H]5-HT through SERT, while it prevented the releasing action of methamphetamine. 5-MeO-DIPT induced cell toxicity at high concentrations in COS-7 cells, and it was not influenced by the expression of SERT. These results demonstrated that 5-MeO-DIPT acts as a competitive SERT inhibitor and has an inability to cause reverse transport, underlying its serotonergic actions.
authors: Department of Dental Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Shikata 2-5-1, Okayama 700-8525, Japan.
authors: C
authors: C
authors: Sogawa
authors: N
authors: N
authors: Sogawa
authors: J
authors: J
authors: Tagawa
authors: A
authors: A
authors: Fujino
authors: K
authors: K
authors: Ohyama
authors: M
authors: M
authors: Asanuma
authors: M
authors: M
authors: Funada
authors: S
authors: S
authors: Kitayama
mesh_terms: D008735:5-Methoxytryptamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D015306:Biogenic Monoamines / Q000037:antagonists & inhibitors; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D003042:Cocaine / Q000494:pharmacology; D008297:Male; D008694:Methamphetamine / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D050486:Serotonin Plasma Membrane Transport Proteins / Q000378:metabolism*; D017367:Selective Serotonin Reuptake Inhibitors / Q000494:pharmacology*; D013574:Synaptosomes / Q000187:drug effects* / Q000378:metabolism
chemical_list: D008735:5-Methoxytryptamine; D015306:Biogenic Monoamines; D003042:Cocaine; D008694:Methamphetamine; D050486:Serotonin Plasma Membrane Transport Proteins; D017367:Selective Serotonin Reuptake Inhibitors; C033696:5-methoxy-N,N-diisopropyltryptamine