Immobilization and long-term culturing of mouse embryonic stem cells in collagen-chitosan gel matrix.
We propose a method of creation of a 3D matrix consisting of native collagen fibers and natural polysaccharide chitosan. The collagen-chitosan hydrogels maintain viability and prolipherative activity of embryonic stem cells obtained from internal cells of mouse blastocyst. The proposed system forming hydrogels in situ can be used in cell therapy for immobilization and targeted delivery of stem cells.
Selezneva
I I
II
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino. selezneva_i@mail.ru
Savintseva
I V
IV
Vikhlyantseva
E F
EF
Davydova
G A
GA
Gavrilyuk
B K
BK
D000818:Animals; D001755:Blastocyst / Q000166:cytology; D018929:Cell Culture Techniques / Q000379:methods*; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D048271:Chitosan / Q000737:chemistry; D003094:Collagen / Q000737:chemistry; D053595:Embryonic Stem Cells / Q000166:cytology*; D020100:Hydrogels / Q000737:chemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA
D020100:Hydrogels; D003094:Collagen; D048271:Chitosan