title: Creating ultrathin nanoscopic collagen matrices for biological and biotechnological applications.
abstract: The biofunctionalization of materials creates interfaces on which proteins, cells, or tissues can fulfill native or desired tasks. Here we report how to control the assembly of type I collagen into well-defined nanoscopic matrices of different patterns. Collagen fibrils in these ultrathin (approximately 3 nm) matrices maintained their native structure as observed in vivo. This opens up the possibility to create programmable biofunctionalized matrices using collagen-binding proteins or proteins fused with collagen-binding domains. Applied to eukaryotic cells, these nanostructured matrices can direct cellular processes such as adhesion, orientation and migration.
authors: BIOTEC, University of Technology Dresden, Tatzberg 47-51, 01307 Dresden, Germany.
authors: David A
authors: DA
authors: Cisneros
authors: Jens
authors: J
authors: Friedrichs
authors: Anna
authors: A
authors: Taubenberger
authors: Clemens M
authors: CM
authors: Franz
authors: Daniel J
authors: DJ
authors: Muller
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D001709:Biotechnology / Q000295:instrumentation*; D002021:Buffers; D002448:Cell Adhesion; D002465:Cell Movement; D016764:Cell Polarity; D004573:Electrolytes; D024022:Fibrillar Collagens / Q000737:chemistry* / Q000648:ultrastructure*; D006863:Hydrogen-Ion Concentration; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry*; D020836:Protein Structure, Quaternary; D051381:Rats
chemical_list: D002021:Buffers; D004573:Electrolytes; D024022:Fibrillar Collagens