title: High-throughput proteomics processing of proteins in polyacrylamide in a multiwell format.
abstract: Processing multiple protein samples from polyacrylamide at significant sensitivity represents a major chokepoint for raising the success rate in high-volume protein identification projects. A multiwell filterplate method for processing proteins in polyacrylamide was optimized for sensitivity using a protein standard. The results demonstrate this process to be a reliable and reproducible method over a range of gel loadings and suitable for the identification of proteins near the threshold of silver stain. This high-throughput manual method requires a minimum of specialized equipment, and can be performed disconnected from a proteomics infrastructure for the preparation of mass spectrometry-ready samples.
authors: Center of Excellence in Genomics and Bioinformatics, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA.
authors: Edwin D
authors: ED
authors: Cummings
authors: Janet M
authors: JM
authors: Brown
authors: Siva T
authors: ST
authors: Sarva
authors: Robert H
authors: RH
authors: Waldo
authors: George M
authors: GM
authors: Hilliard
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D008969:Molecular Sequence Data; D010449:Peptide Mapping / Q000379:methods* / Q000592:standards; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods* / Q000592:standards; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000302:isolation & purification; D014357:Trypsin / Q000737:chemistry; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D011506:Proteins; D012710:Serum Albumin, Bovine; D014357:Trypsin; D000099166:Polyacrylamides