Protective effects of pLNCX-SOD gene transfection on hepatocyte injury induced by obstructive jaundice in rats.
BACKGROUND
Hydrophobic bile acids lead to the generation of oxygen free radicals in mitochondria. Accordingly, this study is to investigate if gene delivery of superoxide dismutase (SOD) will reduce hepatocyte injury caused by experimental cholestasis.


METHODS
The recombinant of pLNCX-SOD gene packaged with lipofection of AMINE was transfected into hepatocytes in vitro, which stably expressed the SOD gene.


RESULTS
After transfection, hepatocytes enhanced the protective effect against injury to bile and the toxicity of serum in obstructive jaundice. The inhibition of bile at the concentration of 2% (v:v, bile: DMEM 1:50) decreased obviously from (78.80+/-12.35)% to (43.35+/-9.69)% in 12 hours, from (82.55+/-11.27)% to (-26.64+/-7.66)% in 24 hours, and from (83.83+/-18.69)% to (-19.27+/-14.38)% in 48 hours, compared with that of the untransfected cells (P<0.01). The inhibition of serum at the obstructive jaudice concentration of 2.5% was obviously decreased from (89.72+/-1.52)% to (14.68+/-14.33)% in 12 hours, from (92.2+/-11.27)% to (41.39+/-7.66)% in 24 hours, and from (94.25+/-8.96)% to (22.71+/-4.38)% in 48 hours (P<0.01).


CONCLUSIONS
Hepatocytes transfected with the pLNCX-SOD gene could obviously be resistant to the toxicity of bile and serum from rat with obstructive jaundice.
Gong
Peng
P
Department of General Surgery, the First Affiliated Hospital, Dalian Medical University, Dalian, China. gongp28@263.net
Wang
Zhong-Yu
ZY
Wang
Hong-Jiang
HJ
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D004195:Disease Models, Animal; D016166:Free Radical Scavengers / Q000627:therapeutic use*; D015316:Genetic Therapy; D022781:Hepatocytes / Q000473:pathology*; D041781:Jaundice, Obstructive / Q000473:pathology / Q000628:therapy*; D008297:Male; D010957:Plasmids; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000627:therapeutic use*; D014162:Transfection
D016166:Free Radical Scavengers; D013482:Superoxide Dismutase