Female-specific DNA sequences in the chicken genome.
Eight in silico W-specific sequences from the WASHUC1 chicken genome assembly gave female-specific PCR products using chicken DNA. Some of these fragments gave female-specific products with turkey and peacock DNA. Sequence analysis of these 8 fragments (3077 bp total) failed to detect any polymorphisms among 10 divergent chickens. In contrast, comparison of the DNA sequences of chicken with those of turkey and peacock revealed a nucleotide difference every 25 and 28 bp, respectively. Radiation hybrid mapping verified that these amplicons exist only on chromosome W. The homology of 6 W-specific fragments with chromo-helicase-DNA-binding gene and expressed sequenced tags from chicken and other species indicate that these fragments may have or have had a biological function. These fragments may be used for early sexing in commercial chicken and turkey flocks.
Granevitze
Zur
Z
Robert H. Smith Institute of Plant Sciences and Genetics, The Hebrew University of Jerusalem, Rehovot 76100, Israel.
Blum
Shula
S
Cheng
Hans
H
Vignal
Alain
A
Morisson
Mireille
M
Ben-Ari
Giora
G
David
Lior
L
Feldman
Marcus William
MW
Weigend
Steffen
S
Hillel
Jossi
J
D000818:Animals; D002645:Chickens / Q000235:genetics*; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics*; D005260:Female; D016678:Genome; D006822:Hybrid Cells; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide
D004247:DNA