[Case report of a rare platelet-specific antigen HPA-10bw allele found in Chinese mainland].
A total of 1,000 Chinese blood donors were typed for human platelet antigens (HPA) using a sequence specific primers -polymerase chain reaction (PCR-SSP) based HPA genotyping method. An individual with a rare HPA-10w(a+b+) genotype was found. In order to confirm the typing results, a fragment of HPA-10 gene was amplified by PCR and then sequenced. Sequencing data showed that a single G to A substitution at nucleotide 263 occurred, resulting in amino acid change from Arg(CGA) to Gln(CAA) at position 62 of GPa protein. The substitution generated antigenic specificity HPA-10bw. The detection of an HPA-10bw allele in the Chinese population suggests that this rare allele should be considered in platelet alloimmunization, such as neonatal alloimmune thrombocytopenia (NAIT), post-transfusion thrombocytopenic purpura (PTP) and post-transfusion refractoriness to platelets (PTR).
Feng
Ming-Liang
ML
Shanghai Blood Center, Shanghai, China. fengml@sh163.net
Shen
Tong
T
Huang
Hui
H
Shen
Wei
W
Wang
Jian-Lian
JL
Liu
Da-Zhuang
DZ
Zhao
Tong-Mao
TM
D000483:Alleles; D016824:Antigens, Human Platelet / Q000235:genetics*; D001483:Base Sequence; D002681:China; D005838:Genotype; D006801:Humans; D039661:Integrin beta3 / Q000235:genetics; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
D016824:Antigens, Human Platelet; D039661:Integrin beta3