title: Contact sex pheromone components of the cowpea weevil, Callosobruchus maculatus.
abstract: The cowpea weevil, Callosobruchus maculatus, is a major pest of stored pulses. Females of this species produce a contact sex pheromone that elicits copulation behavior in males. Pheromone was extracted from filter-paper shelters taken from cages that housed females. Crude ether extract stimulated copulation in male C. maculatus. Initial fractionation showed behavioral activity in acidic and neutral fractions. Furthermore, bioassay-guided fractionation and gas chromatography-mass spectroscopy (GC-MS) analysis of active fractions revealed that the active components of the acidic fraction were 2,6-dimethyloctane-1,8-dioic acid and nonanedioic acid. These components along with the hydrocarbon fraction, a mixture of C(27)-C(35) straight chain and methyl branched hydrocarbons, had a synergistic effect on the behavior of males. Glass dummies treated with an authentic pheromone blend induced copulation behavior in males. The potential roles of the contact sex pheromone of C. maculatus are discussed.
authors: Laboratory of Biofunctional Molecular Analysis, Department of Bioscience, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1-1-1, Sakuragaoka, Setagaya-ku, Tokyo, 156-8502, Japan. toshi_nojima@ncsu.edu
authors: Satoshi
authors: S
authors: Nojima
authors: Kenji
authors: K
authors: Shimomura
authors: Hiroshi
authors: H
authors: Honda
authors: Izuru
authors: I
authors: Yamamoto
authors: Kanju
authors: K
authors: Ohsawa
mesh_terms: D000080462:Acyclic Monoterpenes; D000818:Animals; D003998:Dicarboxylic Acids / Q000032:analysis / Q000494:pharmacology; D005260:Female; D006838:Hydrocarbons / Q000032:analysis / Q000494:pharmacology; D008297:Male; D012724:Sex Attractants / Q000302:isolation & purification* / Q000494:pharmacology*; D012726:Sexual Behavior, Animal / Q000187:drug effects* / Q000502:physiology; D048889:Weevils / Q000502:physiology*
chemical_list: D000080462:Acyclic Monoterpenes; D003998:Dicarboxylic Acids; D006838:Hydrocarbons; D012724:Sex Attractants; C037294:2,6-dimethyloctane-1,8-dioic acid; C010038:azelaic acid