15N metabolic labeling of mammalian tissue with slow protein turnover.
We previously reported the metabolic 15N labeling of a rat where enrichment ranged from 94% to 74%. We report here an improved labeling strategy which generates 94% 15N enrichment throughout all tissues of the rat. A high 15N enrichment of the internal standard is necessary for accurate quantitation, and thus, this approach will allow quantitative mass spectrometry analysis of animal models of disease targeting any tissue.
McClatchy
Daniel B
DB
Department of Cell Biology, 10550 North Torrey Pines Road, SR11, The Scripps Research Institute, LaJolla, California 92037, USA.
Dong
Meng-Qiu
MQ
Wu
Christine C
CC
Venable
John D
JD
Yates
John R
JR
D000818:Animals; D004032:Diet; D005260:Female; D009587:Nitrogen Isotopes / Q000737:chemistry / Q000378:metabolism*; D011247:Pregnancy; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling / Q000379:methods*; D053719:Tandem Mass Spectrometry
D009587:Nitrogen Isotopes; D011506:Proteins