title: Protective effects of pLNCX-SOD gene transfection on hepatocyte injury induced by obstructive jaundice in rats.
abstract: BACKGROUND
  Hydrophobic bile acids lead to the generation of oxygen free radicals in mitochondria. Accordingly, this study is to investigate if gene delivery of superoxide dismutase (SOD) will reduce hepatocyte injury caused by experimental cholestasis.
  
  
  METHODS
  The recombinant of pLNCX-SOD gene packaged with lipofection of AMINE was transfected into hepatocytes in vitro, which stably expressed the SOD gene.
  
  
  RESULTS
  After transfection, hepatocytes enhanced the protective effect against injury to bile and the toxicity of serum in obstructive jaundice. The inhibition of bile at the concentration of 2% (v:v, bile: DMEM 1:50) decreased obviously from (78.80+/-12.35)% to (43.35+/-9.69)% in 12 hours, from (82.55+/-11.27)% to (-26.64+/-7.66)% in 24 hours, and from (83.83+/-18.69)% to (-19.27+/-14.38)% in 48 hours, compared with that of the untransfected cells (P<0.01). The inhibition of serum at the obstructive jaudice concentration of 2.5% was obviously decreased from (89.72+/-1.52)% to (14.68+/-14.33)% in 12 hours, from (92.2+/-11.27)% to (41.39+/-7.66)% in 24 hours, and from (94.25+/-8.96)% to (22.71+/-4.38)% in 48 hours (P<0.01).
  
  
  CONCLUSIONS
  Hepatocytes transfected with the pLNCX-SOD gene could obviously be resistant to the toxicity of bile and serum from rat with obstructive jaundice.
authors: Department of General Surgery, the First Affiliated Hospital, Dalian Medical University, Dalian, China. gongp28@263.net
authors: Peng
authors: P
authors: Gong
authors: Zhong-Yu
authors: ZY
authors: Wang
authors: Hong-Jiang
authors: HJ
authors: Wang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D004195:Disease Models, Animal; D016166:Free Radical Scavengers / Q000627:therapeutic use*; D015316:Genetic Therapy; D022781:Hepatocytes / Q000473:pathology*; D041781:Jaundice, Obstructive / Q000473:pathology / Q000628:therapy*; D008297:Male; D010957:Plasmids; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000627:therapeutic use*; D014162:Transfection
chemical_list: D016166:Free Radical Scavengers; D013482:Superoxide Dismutase