title: [Influential factor analysis and improvement of cultural method of oral mucosal epithelial cells].
abstract: OBJECTIVE
  To analyze influential factors of cultural method of human oral mucosal epithelial cells (hOMEC)and to establish a reliable cell culture system for hOMEC.
  
  
  METHODS
  Benzalkonium bromide and gentamicin sulfate were used to prevent microbial contamination. Separations of epithelium from underlying connective tissues with Dispase at different concentration were compared. Enzyme digestion was used to isolate cells and keratinocyte-serum free medium(K-SFM) was employed for primary culture and subculture of hOMEC.
  
  
  RESULTS
  Microbial contamination was under control. Separation of epithelium from underlying connective tissues with 0.40% Dispase was more complete than that of 0.25% Dispase. The cells grew fast and well in vitro.
  
  
  CONCLUSIONS
  The high successful culture of hOMEC and simplified procedures could be obtained with improvement of methods.
authors: Dept. of Oral Medicine, School of Stomatology, Jilin University, Changchun 130041, China.
authors: Xiang
authors: X
authors: Wang
authors: Wen-xin
authors: WX
authors: Gao
authors: Li
authors: L
authors: Wang
authors: Xiu-feng
authors: XF
authors: Wei
authors: Ying-xin
authors: YX
authors: Chen
authors: Xiao-feng
authors: XF
authors: Wang
mesh_terms: D018929:Cell Culture Techniques; D004847:Epithelial Cells; D004848:Epithelium; D005163:Factor Analysis, Statistical; D006801:Humans; D015603:Keratinocytes; D009061:Mouth Mucosa