Detection of altered N-glycan profiles in whole serum from rheumatoid arthritis patients.
Altered N-glycosylation occurs in many diseases. In rheumatoid arthritis (RA), for example, reduction in galactose residues in IgG and an increase in fucose residues in alpha1-acid glycoprotein have been observed. To further analyse N-glycans in disease, we show N-glycan profiling from whole serum employing reversed phase high performance liquid chromatography/negative-ion mode by sonic spray ionization ion trap mass spectrometry with pyridylamination. Profiles from female 15 RA patients and 18 aged-matched healthy women were compared. The most significant change seen in RA was decreased levels of mono-galactosyl bi-antennary N-glycans, in agreement with the previous reports regarding IgG. We also show previously unreported differences between isomers and increased tri-antennary oligosaccharides. These results indicate that LC-MS analysis of whole serum N-glycans can identify N-glycan alterations in RA and that this is a promising method both for studies of RA mechanisms and diagnosis.
Nakagawa
Hiroaki
H
Graduate School of Advanced Life Science, Frontier Research Center for Post-Genomic Science and Technology, Hokkaido University, W11 N21, Sapporo 001-0021, Japan. nakagawa@glyco.sci.hokudai.ac.jp
Hato
Megumi
M
Takegawa
Yasuhiro
Y
Deguchi
Kisaburo
K
Ito
Hiroki
H
Takahata
Masahiko
M
Iwasaki
Norimasa
N
Minami
Akio
A
Nishimura
Shin-Ichiro
S
D001172:Arthritis, Rheumatoid / Q000097:blood*; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D008875:Middle Aged; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000097:blood* / Q000737:chemistry; D015203:Reproducibility of Results
D011134:Polysaccharides