Single-cell time-lapse imaging of the dynamic control of NF-kappaB signalling.
The transcription factor NF-kappaB (nuclear factor kappaB) regulates critical cellular processes including the inflammatory response, apoptosis and the cell cycle. Over the past 20 years many of the components of the NF-kappaB signalling pathway have been elucidated along with their functions. Recent research in this field has focused on the dynamic regulation and network control of this system. With key roles in so many important cellular processes, it is critical that NF-kappaB signalling is tightly regulated. Recently, single-cell imaging and mathematical modelling have identified that the timing of cellular responses may play an important role in the regulation of this pathway. p65/RelA (RelA) has been shown to translocate between the nucleus and cytoplasm with varying oscillatory patterns in different cell lines leading to differences in transcriptional outputs from NF-kappaB-regulated genes. Variations in the timing or persistence of these movements may control the maintenance and differential expression of NF-kappaB-regulated genes.
Sillitoe
K
K
School of Biological Sciences, University of Liverpool, Bioscience Research Building, Crown St, Liverpool, UK.
Horton
C
C
Spiller
D G
DG
White
M R H
MR
D006367:HeLa Cells; D006801:Humans; D020672:I-kappa B Proteins / Q000502:physiology*; D007700:Kinetics; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000502:physiology*; D009991:Oscillometry; D011499:Protein Processing, Post-Translational; D015398:Signal Transduction; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology
D020672:I-kappa B Proteins; D016328:NF-kappa B; C000606204:NFKBIA protein, human; D014409:Tumor Necrosis Factor-alpha; D000072000:NF-KappaB Inhibitor alpha