title: In vivo evidence for quasispecies distributions in the bovine respiratory syncytial virus genome.
abstract: We analysed the genetic evolution of bovine respiratory syncytial virus (BRSV) isolate W2-00131, from its isolation in bovine turbinate (BT) cells to its inoculation in calves. Results showed that the BRSV genomic region encoding the highly variable glycoprotein G remained genetically stable after virus isolation and over 10 serial infections in BT cells, as well as following experimental inoculation in calves. This remarkable genetic stability led us to examine the mutant spectrum of several populations derived from this field isolate. Sequence analysis of molecular clones revealed an important genetic heterogeneity in the G-coding region of each population, with mutation frequencies ranging from 6.8 to 10.1 x 10(-4) substitutions per nucleotide. The non-synonymous mutations of the mutant spectrum mapped preferentially within the two variable antigenic regions of the ectodomain or close to the highly conserved domain. These results suggest that BRSV populations may evolve as complex and dynamic mutant swarms, despite apparent genetic stability.
authors: INRA-ENVT, UMR1225 - Interactions Hosts-Pathogens (IHAP), Ecole Nationale Vétérinaire, 31076 Toulouse cedex 03, France.
authors: Martine
authors: M
authors: Deplanche
authors: Laboratoire Départemental Vétérinaire LVD09, 09007 Foix cedex, France.
authors: Mylène
authors: M
authors: Lemaire
authors: INRA-ENVT, UMR1225 - Interactions Hosts-Pathogens (IHAP), Ecole Nationale Vétérinaire, 31076 Toulouse cedex 03, France.
authors: Carole
authors: C
authors: Mirandette
authors: INRA-ENVT, UMR1225 - Interactions Hosts-Pathogens (IHAP), Ecole Nationale Vétérinaire, 31076 Toulouse cedex 03, France.
authors: Marion
authors: M
authors: Bonnet
authors: INRA-ENVT, UMR1225 - Interactions Hosts-Pathogens (IHAP), Ecole Nationale Vétérinaire, 31076 Toulouse cedex 03, France.
authors: François
authors: F
authors: Schelcher
authors: INRA-ENVT, UMR1225 - Interactions Hosts-Pathogens (IHAP), Ecole Nationale Vétérinaire, 31076 Toulouse cedex 03, France.
authors: Gilles
authors: G
authors: Meyer
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology; D016679:Genome, Viral; D009154:Mutation; D011110:Polymorphism, Genetic; D017434:Protein Structure, Tertiary; D018357:Respiratory Syncytial Virus Infections / Q000662:veterinary / Q000821:virology; D018112:Respiratory Syncytial Virus, Bovine / Q000145:classification* / Q000235:genetics* / Q000276:immunology; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D000956:Antigens, Viral; C066028:G protein, Bovine respiratory syncytial virus; D014759:Viral Envelope Proteins