title: Plasmodium berghei: plasmodium perforin-like protein 5 is required for mosquito midgut invasion in Anopheles stephensi.
abstract: During its life cycle the malarial parasite Plasmodium forms three invasive stages which have to invade different and specific cells for replication to ensue. Invasion is vital to parasite survival and consequently proteins responsible for invasion are considered to be candidate vaccine/drug targets. Plasmodium perforin-like proteins (PPLPs) have been implicated in invasion because they contain a predicted pore-forming domain. Ookinetes express three PPLPs, and one of them (PPLP3) has previously been shown to be essential for mosquito midgut invasion. In this study we show through phenotypic analysis of loss-of-function mutants that PPLP5 is equally essential for mosquito infection. Deltapplp5 ookinetes cannot invade midgut epithelial cells, but subsequent parasite development is rescued if the midgut is bypassed by injection of ookinetes into the hemocoel. The indistinguishable phenotypes of Deltapplp5 and Deltapplp3 ookinetes strongly suggest that these two proteins contribute to a common process.
authors: Division of Cell and Molecular Biology, Faculty of Natural Sciences, Imperial College London, Sir Alexander Fleming Building, Imperial College Road, London, UK. andrea.ecker@imperial.ac.uk <andrea.ecker@imperial.ac.uk>
authors: Andrea
authors: A
authors: Ecker
authors: Sofia B
authors: SB
authors: Pinto
authors: Ken W
authors: KW
authors: Baker
authors: Fotis C
authors: FC
authors: Kafatos
authors: Robert E
authors: RE
authors: Sinden
mesh_terms: D000818:Animals; D000852:Anopheles / Q000469:parasitology*; D006790:Host-Parasite Interactions / Q000502:physiology; D007303:Insect Vectors / Q000469:parasitology*; D008288:Malaria / Q000635:transmission*; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D010962:Plasmodium berghei / Q000502:physiology*; D015800:Protozoan Proteins / Q000235:genetics / Q000502:physiology*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000502:physiology*
chemical_list: C521556:Plasmodium perforin-like protein 5; D015800:Protozoan Proteins; D011956:Receptors, Cell Surface