title: Polymeric nanoparticles for protein kinase activity.
abstract: Nanoparticles were prepared from poly-ion complexes, possessing both PEI-FITC-(PKA-specific substrate) (kemptide) and PAA-TRITC, which produce intermolecular FRET; the nanoparticles were dissociated by phosphorylation, presented a strong FITC intensity and can be applied to high-throughput screening for large chemical libraries, for drug discovery of kinase inhibitors.
authors: Biomedical Research Center, Korea Institute of Science and Technology, 39-1 Hawolgok-dong, Seongbuk-gu, Seoul 136-791, South Korea.
authors: Jong-Ho
authors: JH
authors: Kim
authors: Seulki
authors: S
authors: Lee
authors: Kwangmeyung
authors: K
authors: Kim
authors: Hyesung
authors: H
authors: Jeon
authors: Rang-Woon
authors: RW
authors: Park
authors: In-San
authors: IS
authors: Kim
authors: Kuiwon
authors: K
authors: Choi
authors: Ick Chan
authors: IC
authors: Kwon
mesh_terms: D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*
chemical_list: D011108:Polymers; D017868:Cyclic AMP-Dependent Protein Kinases