Determination of dye precursors in hair coloring products by liquid chromatography with electrochemical detection.
The simultaneous determination of seven aminophenols, resorcinol and p-phenylenediamine in hair coloring products was performed by liquid chromatography (HPLC) with amperometric detection (ED). The aminophenols were separated on a ODS C18 reversed-phase column by isocratic elution with a mobile phase based on 0.1 M acetate buffer pH 4.5-methanol (90:10%, v/v) at a flow rate 0.8 mL min(-1). The limit of detection (S/N=3) for the aminophenols was in the 15-40 pg (injected mass) range at an applied potential of 0.950 V versus Ag/AgCl. Peak heights for the aminophenols and the two others compounds were found to be linearly related to the amount injected, from 0.3 to 300 ng (r>0.994-0.999). The relative standard deviation (R.S.D., n=10) for 1 ng injected was comprised in the range from 2.5 to 6.2%, depending on the aminophenol tested. The present method minimizes troublesome and time-consuming pretreatment procedures and it was applied to the determination of aminophenols, resorcinol and phenylenediamine in hair coloring formulations.
Narita
Motoko
M
Tokyo Kasei University, Faculty of Domestic Science, 1-18-1, Kaga, Itabashi, Tokyo 178-8602, Japan.
Murakami
Kazuo
K
Kauffmann
Jean-Michel
JM
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004396:Coloring Agents / Q000032:analysis*; D004563:Electrochemistry / Q000379:methods*; D006203:Hair Preparations / Q000737:chemistry*; D012015:Reference Standards
D004396:Coloring Agents; D006203:Hair Preparations