Single cell fluorescence imaging using metal plasmon-coupled probe.
This work constitutes the first fluorescent imaging of cells using metal plasmon-coupled probes (PCPs) at single cell resolution. N-(2-Mercapto-propionyl)glycine-coated silver nanoparticles were synthesized by reduction of silver nitrate using sodium borohyride and then succinimidylated via ligand exchange. Alexa Fluor 647-labeled concanavalin A (con A) was chemically bound to the silver particles to make the fluorescent metal plasmon-coupled probes. The fluorescence images were collected using a scanning confocal microscopy. The fluorescence intensity was observed to enhance 7-fold when binding the labeled con A on a single silver particle. PCPs were conjugated on HEK 293 A cells. Imaging results demonstrate that cells labeled by PCPs were 20-fold brighter than those by free labeled con A.
Zhang
Jian
J
Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA.
Fu
Yi
Y
Lakowicz
Joseph R
JR
D002478:Cells, Cultured; D003208:Concanavalin A / Q000737:chemistry; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000737:chemistry; D005453:Fluorescence; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D015335:Molecular Probes / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D020349:Surface Plasmon Resonance; D008625:Tiopronin / Q000737:chemistry*
D015335:Molecular Probes; D003208:Concanavalin A; C067297:8-aminohexylamino cAMP; D012834:Silver; D008625:Tiopronin; D000242:Cyclic AMP