Improving retting of fibre through genetic modification of flax to express pectinases.
Flax (Linum usitatissimum L.) is a raw material used for important industrial products. Linen has very high quality textile properties, such as its strength, water absorption, comfort and feel. However, it occupies less than 1% of the total textile market. The major reason for this is the long and difficult retting process by which linen fibres are obtained. In retting, bast fibre bundles are separated from the core, the epidermis and the cuticle. This is accomplished by the cleavage of pectins and hemicellulose in the flax cell wall, a process mainly carried out by plant pathogens like filamentous fungi. The remaining bast fibres are mainly composed of cellulose and lignin. The aim of this study was to generate plants that could be retted more efficiently. To accomplish this, we employed the novel approach of transgenic flax plant generation with increased polygalacturonase (PGI ) and rhamnogalacturonase (RHA) activities. The constitutive expression of Aspergillus aculeatus genes resulted in a significant reduction in the pectin content in tissue-cultured and field-grown plants. This pectin content reduction was accompanied by a significantly higher (more than 2-fold) retting efficiency of the transgenic plant fibres as measured by a modified Fried's test. No alteration in the lignin or cellulose content was observed in the transgenic plants relative to the control. This indicates that the over-expression of the two enzymes does not affect flax fibre composition. The growth rate and soluble sugar and starch contents were in the range of the control levels. It is interesting to note that the RHA and PGI plants showed higher resistance to Fusarium culmorum and F. oxysporum attack, which correlates with the increased phenolic acid level. In this report, we demonstrate for the first time that over-expression of the A. aculeatus genes results in flax plants more readily usable for fibre production. The biochemical parameters of the cell wall components indicated that the fibre quality remains similar to that of wild-type plants, which is an important pre-requisite for industrial applications.
Musialak
Magdalena
M
Faculty of Biotechnology, University of Wroclaw, Przybyszewskiego 63, 51-148 Wroclaw, Poland.
Wróbel-Kwiatkowska
Magdalena
M
Kulma
Anna
A
Starzycka
Eligia
E
Szopa
Jan
J
D001230:Aspergillus / Q000201:enzymology / Q000235:genetics; D001483:Base Sequence; D002482:Cellulose / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D019597:Flax / Q000201:enzymology* / Q000235:genetics* / Q000382:microbiology; D005670:Fusarium / Q000472:pathogenicity; D015870:Gene Expression; D005800:Genes, Fungal; D062385:Hydroxybenzoates / Q000378:metabolism; D008031:Lignin / Q000378:metabolism; D010368:Pectins / Q000378:metabolism; D030821:Plants, Genetically Modified; D011096:Polygalacturonase / Q000235:genetics*; D013784:Textiles / Q000032:analysis*
D017931:DNA Primers; D062385:Hydroxybenzoates; D010368:Pectins; D002482:Cellulose; D008031:Lignin; D011096:Polygalacturonase; C017616:phenolic acid