Induction of immunological antitumor effects by adenovirus-mediated gene transfer of B7-1 in a murine squamous cell carcinoma cell line.
OBJECTIVE
To evaluate the antitumor immune effects of B7-1 gene expression mediated by adenoviral vectors against squamous cell carcinoma. Transfection of the costimulatory molecule B7-1 gene into certain murine tumors increases antitumor immunity and suppresses tumor growth.


METHODS
In vitro and in vivo study.


METHODS
A murine squamous cell carcinoma cell line, KLN205, was infected with adenoviral vectors carrying either B7-1 (AdB7) or LacZ (AdCL) genes. Infected cells were injected subcutaneously into the flanks of DBA/2 mice.


METHODS
The growth of tumors infected with adenviral vectors was measured.


RESULTS
AdB7-infected cells grew significantly slower than AdCL-infected cells in vivo, while there was no significant difference in the growth rates between the 2 groups in vitro. Moreover, significant growth suppression of rechallenged noninfected parental cells was observed in the mice immunized with AdB7-infected cells but not in those immunized with AdCL-infected cells.


CONCLUSIONS
These results suggest that the B7-1 gene has therapeutic potential for immunotherapy against head and neck squamous cell carcinoma.
Hoshitani
Yasuhiko
Y
Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, International Center for Medical Research and Treatment, School of Medicine, Kobe University, Kobe, Japan.
Ishida
Haruhiko
H
Otsuki
Naoki
N
Shirakawa
Toshiro
T
Gotoh
Akinobu
A
Nibu
Ken-Ichi
K
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D018122:B7-1 Antigen / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000276:immunology*; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D015870:Gene Expression; D005822:Genetic Vectors; D006258:Head and Neck Neoplasms / Q000188:drug therapy / Q000276:immunology*; D016233:Immunotherapy, Active / Q000379:methods; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D014162:Transfection
D018122:B7-1 Antigen