The gene expression profiling in murine cortical cells undergoing programmed cell death (PCD) induced by serum deprivation.
PCD (programmed cell death) is important mechanism for development, homeostasis and disease. To analyze the gene expression pattern in brain cells undergoing PCD in response to serum deprivation, we analyzed the cDNA microarray consisting of 2,300 genes and 7 housekeeping genes of cortical cells derived from mouse embryonic brain. Cortical cells were induced apoptosis by serum deprivation for 8 hours. We identified 69 up-regulated genes and 21 down-regulated genes in apoptotic cells. Based on the cDNA microarray data four genes were selected and analyzed by RT-PCR and northern blotting. To characterize the role of UNC-51-like kinase (ULK2) gene in PCD, we investigated cell death effect by ULK2. And we examined expression of several genes that related with PCD. Especially GAPDH was increased by ULK2. Theses findings indicated that ULK2 is involved in apoptosis through p53 pathway.
Yang
Moon Hee
MH
Department of Biological Science, Sookmyung Womenos University, Seoul 140-742, Korea.
Yoo
Kyung Hyun
KH
Yook
Yeon Joo
YJ
Park
Eun Young
EY
Jeon
Jeong Ok
JO
Choi
Seo Hee
SH
Park
So Young
SY
Woo
Yu Mi
YM
Lee
Min Joo
MJ
Park
Jong Hoon
JH
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects; D020869:Gene Expression Profiling; D051379:Mice; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D044967:Serum / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects
D012333:RNA, Messenger; C401061:Ulk2 protein, mouse; D017346:Protein Serine-Threonine Kinases