Correction of inner-filter effect in fluorescence excitation-emission matrix spectrometry using Raman scatter.
Fluorescence excitation-emission matrix (EEM) spectroscopy is a useful tool for interpretation of fluorescence information from natural water samples. One of the major problems with this technique is the inner-filter effect (IFE), i.e. absorption of light at both the excitation and emission wavelengths. The common solutions are to either dilute the sample or apply some form of mathematical correction, most often based on the measured absorbance of the sample. Since dilution is not always possible, e.g. in on-line or in situ EEM recordings, and corrections based on absorbance are hampered primarily by the use of a separate absorbance instrument, neither of these solutions is optimal. In this work, we propose a mathematical correction procedure based on the intensity of Raman scatter from water. This procedure was found to reduce the error after correction by up to 50% in comparison with two absorbance correction procedures. Furthermore, it does not require the use of a separate absorbance measurement, and it is applicable to on-line and in situ EEM recordings, where the IFE would otherwise cause problems.
Larsson
Tobias
T
Department of Chemistry, Göteborg University, Kemivägen 10, SE-41296 Göteborg, Sweden. toblar@chem.gu.se
Wedborg
Margareta
M
Turner
David
D
D005374:Filtration / Q000295:instrumentation / Q000592:standards; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods / Q000592:standards; D013059:Spectrum Analysis, Raman / Q000295:instrumentation* / Q000379:methods* / Q000592:standards