title: Effects of two fluid resuscitations on the bacterial translocation and inflammatory response of small intestine in rats with hemorrhagic shock.
abstract: OBJECTIVE
  To investigate the effects of two fluid resuscitations on the bacterial translocation and the inflammatory factors of small intestine in rats with hemorrhagic shock.
  
  
  METHODS
  Fifty SD healthy male rats were randomly divided into 5 groups (n equal to 10 per group): Group A (Sham group), Group B (Ringer's solution for 1 h), Group C (Ringer's solution for 24 h), Group D (hydroxyethyl starch for 1 h) and Group E ((hydroxyethyl starch for 24 h). A model of rats with hemorrhagic shock was established. The bacterial translocation in liver, content of tumor necrosis factor-alpha (TNF-alpha) and changes of myeloperoxidase enzyme (MPO) activities in small intestine were pathologically investigated after these two fluid resuscitations, respectively.
  
  
  RESULTS
  The bacterial translocation and the expression of TNF-alpha in the small intestine were detected at 1 h and 24 h after fluid resuscitation. There were significant increase in the number of translocated bacteria, TNF-alpha and MPO activities in Group C compared with Group B, significant decrease in Group E compared with Group D and in Group B compared with Group D. The number of translocated bacteria and TNF-alpha expression significantly decreased in Group E as compared with Group C.
  
  
  CONCLUSIONS
  The bacterial translocation and the expression of TNF-alpha in the small intestine exist 24 h after fluid resuscitation. 6% hydroxyethyl starch can improve the intestinal mucosa barrier function better than the Ringer's solution.
authors: Department of Anesthesiology, the 4th Hospital of Shenzhen, Shenzhen 518033, China. gaodays@163.com
authors: Xin-yao
authors: XY
authors: Gao
authors: Cong-cai
authors: CC
authors: Ren
authors: Qiang
authors: Q
authors: Zhou
authors: Qing-feng
authors: QF
authors: Pang
authors: Chang-yi
authors: CY
authors: Wu
authors: Yin-ming
authors: YM
authors: Zeng
mesh_terms: D000818:Animals; D018988:Bacterial Translocation / Q000187:drug effects*; D005440:Fluid Therapy; D006895:Hydroxyethyl Starch Derivatives / Q000008:administration & dosage / Q000494:pharmacology*; D007421:Intestine, Small / Q000378:metabolism*; D007552:Isotonic Solutions / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077331:Ringer's Solution; D012771:Shock, Hemorrhagic / Q000628:therapy*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D006895:Hydroxyethyl Starch Derivatives; D007552:Isotonic Solutions; D014409:Tumor Necrosis Factor-alpha; D000077331:Ringer's Solution; D009195:Peroxidase