Design of the complex between manganese porphyrins and catalase-poly(ethylene glycol) conjugates for a new antioxidant.
A new design of antioxidant, the complex between manganese (Mn) porphyrins and catalase-poly(ethylene glycol) (PEG) conjugates, is reported. Gel filtration chromatography and a Langmuir-type adsorption isotherm proved that the catalase-PEG conjugate formed the complex with the Mn-porphyrin. The resulting complex exhibited significant superoxide dismutase (SOD) and catalase activity. These results suggest that the Mn-porphyrin/catalase-PEG complex with dual enzymatic activity, i.e., SOD and catalase, is promising for a new class of antioxidants.
Asayama
Shoichiro
S
Department of Applied Chemistry, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo 192-0397, Japan.
Hanawa
Tomochika
T
Nagaoka
Shoji
S
Kawakami
Hiroyoshi
H
D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D002374:Catalase / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology*; D002417:Cattle; D002626:Chemistry, Pharmaceutical; D015195:Drug Design; D066298:In Vitro Techniques; D008665:Metalloporphyrins / Q000737:chemistry* / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology
D000975:Antioxidants; D008665:Metalloporphyrins; D011092:Polyethylene Glycols; D002374:Catalase; D013482:Superoxide Dismutase