Elicitin-responsive lectin-like receptor kinase genes in BY-2 cells.
The inhibition of elicitor-induced plant defense responses by the protein kinase inhibitors K252a and staurosporine indicates that defense responses require protein phosphorylation. We isolated a cDNA clone encoding Nicotiana tabacum lectin-like receptor protein kinase 1 (NtlecRK1), an elicitor-responsive gene; in tobacco bright yellow (BY-2) cells by a differential display method. NtlecRK forms a gene family with at least three members in tobacco. All three NtlecRK genes potentially encode the N-terminal legume lectin domain, transmembrane domain and C-terminal Ser/Thr-type protein kinase domain. Green fluorescent protein (GFP) fusion showed that the NtlecRK1 protein was located on the plasma membrane. In addition, NtlecRK1 and 3 were responsive to INF1 elicitin and the bacterial elicitor harpin. These results indicate that NtlecRKs are membrane-located protein kinases that are induced during defense responses in BY-2 cells.
Sasabe
Michiko
M
Laboratory of Plant Pathology & Genetic Engineering, Graduate School of Natural Science and Technology, Okayama University, Tsushima-naka 1-1-1, Okayama 700-8530, Japan. msasabe@bio.nagoya-u.ac.jp
Naito
Kana
K
Suenaga
Hiroko
H
Ikeda
Takako
T
Toyoda
Kazuhiro
K
Inagaki
Yoshishige
Y
Shiraishi
Tomonori
T
Ichinose
Yuki
Y
D020418:Algal Proteins / Q000494:pharmacology*; D000595:Amino Acid Sequence; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010766:Phosphorylation; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011506:Proteins; D014026:Nicotiana
D020418:Algal Proteins; D010940:Plant Proteins; D011506:Proteins; C085101:elicitin, Phytophthora; D011494:Protein Kinases