Evaluation of toxicity of two pesticides: flucycloxuron and diflubenzuron on a cellular model, Paramecium sp.
The effect of two pesticides, the diflubenzuron (DFB) and the Flucycloxuron (FCX) has been studied on a cellular model: Paramecium sp., a ciliated protiste. The treatment with the DFB in the concentrations of 10 and 20 microg/ml reduces the growth of this protiste appreciably. The survey of the respiratory metabolism by the polarography technique (Oxygen electrode) shows a sensitive inhibition of the oxygen consumption by the studied protiste. In the case of the FCX, the treatment with the two concentrations (10 and 20 microg/ml) reveals an inhibition of the ciliated protiste growth; however, this pesticide inhibits the respiratory metabolism of ciliated protiste. This effect is a lot more marked with the FCX that with the DFB. The coloration with neutral red showed a perturbation in cuticle level, translated by the penetration quantity of the color in treated cells, especially at 20 microg/ml of FCX.
Rouabhi
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R
Department of Biology, University center Larbi Tebessi Tebessa, Algeria. r_rouabhi@yahoo.fr
Berrebbah
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H
Djebar
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MR
D000818:Animals; D001681:Biological Assay; D004132:Diflubenzuron / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D010101:Oxygen Consumption / Q000187:drug effects; D010247:Paramecium / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D010575:Pesticides / Q000633:toxicity*; D010671:Phenylurea Compounds / Q000633:toxicity*; D013997:Time Factors
D010575:Pesticides; D010671:Phenylurea Compounds; D004132:Diflubenzuron; C499258:flucycloxuron