title: Sensitivity of Chlamydia suis to cathelicidin peptides.
abstract: Nine Chlamydia suis isolates, obtained from pigs with conjunctivitis, were molecularly characterized by ompA sequencing and their in vitro susceptibility to six cathelicidin peptides (SMAP-29, BAC-7, BMAP-27, BMAP-27, BMAP-28, PG-1, LL-37) determined in cell culture. SMAP-29 was the most active peptide, reducing the intracellular inclusion number by > or =50% at a concentration of 10 microg/ml (3 microM) in six of the nine isolates tested. Three molecularly identical isolates were insensitive at a concentration as high as 80 microg/ml (25 microM). Of the remaining cathelicidin peptides tested, BAC-7 and BMAP-27 were active against six C. suis isolates at a concentration of 80 microg/ml (25 and 26 microM, respectively). Cathelicidins LL-37 and PG-1 did not show any anti-chlamydial activity at 80 microg/ml.
authors: Sezione di Microbiologia DMCSS, Policlinico S. Orsola, Università di Bologna, Via Massarenti 9, 40138 Bologna, Italy.
authors: Manuela
authors: M
authors: Donati
authors: Antonietta
authors: A
authors: Di Francesco
authors: Renato
authors: R
authors: Gennaro
authors: Monica
authors: M
authors: Benincasa
authors: Simone
authors: S
authors: Magnino
authors: Salvatore
authors: S
authors: Pignanelli
authors: Alisa
authors: A
authors: Shurdhi
authors: Alessandra
authors: A
authors: Moroni
authors: Claudio
authors: C
authors: Mazzoni
authors: Giuseppe
authors: G
authors: Merialdi
authors: Raffaella
authors: R
authors: Baldelli
authors: Roberto
authors: R
authors: Cevenini
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000494:pharmacology*; D054804:Cathelicidins; D002417:Cattle; D002460:Cell Line; D002689:Chlamydia / Q000145:classification* / Q000187:drug effects*; D002522:Chlorocebus aethiops; D006801:Humans; D013552:Swine
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D054804:Cathelicidins