title: Isolation of viral haemorrhagic septicaemia virus from mummichog, stickleback, striped bass and brown trout in eastern Canada.
abstract: Viral haemorrhagic septicaemia virus (VHSV) was isolated from mortalities occurring in populations of mummichog, Fundulus heteroclitus, stickleback, Gasterosteus aculeatus aculeatus, brown trout, Salmo trutta, and striped bass, Morone saxatilis, in New Brunswick and Nova Scotia, Canada. The isolated viral strains produced a cytopathic effect on the epithelioma papillosum cyprini cell line. Serum neutralization indicated the virus was VHSV and sequencing identified the rhabdovirus isolates as the North American strain of VHSV. Phylogenetic analysis indicated that the isolates are closely related and form a distinguishable subgroup of North American type VHSV. To our knowledge, this is the first report of VHSV in mummichog and striped bass.
authors: Department of Fisheries and Oceans, Fish Health Unit, Moncton, NB, Canada. gagnena@dfo-mpo.gc.ca
authors: N
authors: N
authors: Gagné
authors: A-M
authors: AM
authors: Mackinnon
authors: L
authors: L
authors: Boston
authors: B
authors: B
authors: Souter
authors: M
authors: M
authors: Cook-Versloot
authors: S
authors: S
authors: Griffiths
authors: G
authors: G
authors: Olivier
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D001492:Bass / Q000821:virology; D002170:Canada / Q000453:epidemiology; D003532:Cyprinodontiformes / Q000821:virology; D017931:DNA Primers / Q000737:chemistry; D005399:Fishes / Q000821:virology*; D005814:Genes, Viral / Q000235:genetics; D031941:Hemorrhagic Septicemia, Viral / Q000821:virology*; D008969:Molecular Sequence Data; D009500:Neutralization Tests / Q000662:veterinary; D029141:Novirhabdovirus / Q000235:genetics* / Q000302:isolation & purification*; D009698:Nucleoproteins / Q000235:genetics; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment / Q000662:veterinary; D023701:Smegmamorpha / Q000821:virology; D014337:Trout / Q000821:virology
chemical_list: D017931:DNA Primers; D009698:Nucleoproteins