Identification of a putative azadirachtin-binding complex from Drosophila Kc167 cells.
Cell-proliferation in Drosophila Kc167 cells was inhibited by 50% when cell cultures contained 1.7 x 10(-7) M azadirachtin for 48 h (a tertranortriterpenoid from the neem tree Azadirachta indica). Drosophila Kc167 cells exhibited direct nuclear damage within 6-h exposure to azadirachtin (5 x 10(-7) M and above) or within 24 h when lower concentrations were used (1 x 10(-9) M). Fractionation of an extract of Drosophila Kc167 cells combined with ligand overlay technique resulted in the identification of a putative azadirachtin binding complex. Identification of the members of this complex by Peptide Mass Fingerprinting (PMF) and N-terminal sequencing identified heat shock protein 60 (hsp60) as one of its components.
Robertson
Susan L
SL
School of Biological Sciences, Department of Zoology, University of Aberdeen, Aberdeen, United Kingdom.
Ni
Weiting
W
Dhadialla
Tarlochan S
TS
Nisbet
Alasdair J
AJ
McCusker
Catherine
C
Ley
Steven V
SV
Mordue
William
W
Mordue 'Luntz'
A Jennifer
AJ
D000818:Animals; D002457:Cell Extracts / Q000737:chemistry; D002460:Cell Line; D018834:Chaperonin 60 / Q000235:genetics* / Q000378:metabolism; D002850:Chromatography, Gel; D020552:Comet Assay; D019295:Computational Biology; D004249:DNA Damage; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics*; D007306:Insecticides / Q000378:metabolism / Q000633:toxicity*; D036701:Limonins / Q000378:metabolism / Q000633:toxicity*; D046912:Multiprotein Complexes / Q000235:genetics* / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D018709:Statistics, Nonparametric; D014316:Tritium
D002457:Cell Extracts; D018834:Chaperonin 60; D007306:Insecticides; D036701:Limonins; D046912:Multiprotein Complexes; D014316:Tritium; C010329:azadirachtin