[Inhibition of EBV-encoded LMP-1 by DNA-based RNA interference affects metastatic ability of nasopharyngeal carcinoma cells].
OBJECTIVE
To evaluate the application of DNA vector-based RNA interference for silencing LMP-1 expression in EBV-positive nasopharyngeal cancer (NPC) C611 cells and analyze the inhibiting effects of LMP-1 silencing on metastatic property of NPC cells.


METHODS
DNA vectors with LMP-1 shRNA template were transfected into C611 cells. After G418 selection, a LMP-1 silenced sub-clone named C611L was isolated. Effect of LMP-1 inhibition on its metastatic properties was evaluated by cell adhesion, invasion and wound healing assays.


RESULTS
shRNA vector mediated stable RNAi against LMP-1 in C611 cells. Loss of LMP-1 significantly altered cell motility, substratum adhesion and transmembrane invasion ability.


CONCLUSIONS
Stable suppression of LMP-1 induced by shRNA vector can inhibit metastatic properties of NPC cells.
Li
Gang
G
Department of Otolaryngology-Head and Neck Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. climber1999y@yahoo.com.cn
Li
Xiang-Ping
XP
Liu
Xiong
X
Peng
Ying
Y
Lin
Marie C
MC
D015820:Cadherins / Q000378:metabolism; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D005822:Genetic Vectors; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000254:growth & development; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism / Q000473:pathology / Q000821:virology; D009362:Neoplasm Metastasis; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection; D014763:Viral Matrix Proteins / Q000235:genetics* / Q000502:physiology
D015820:Cadherins; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D034741:RNA, Small Interfering; D014763:Viral Matrix Proteins