title: Establishment and characterization of porcine Sertoli cell line for the study of xenotransplantation.
abstract: BACKGROUND
  An understanding of the main mechanism that determines the ability of immune privilege related to Sertoli cells (SC) will provide clues for promoting a local tolerogenic environment. In this report, we established neonatal porcine SC line and evaluated their characteristics.
  
  
  METHODS
  SC line was established following the transfection of primary SC (NPSC) from the testis of neonatal pig with plasmid pRNS-1 carrying genes for neomycin resistance and the SV40 large T antigen. Immunohistochemistry and RT-PCR were performed to evaluate the character of immortalized SC lines.
  
  
  RESULTS
  Our immortalized SC line (iPS) proliferated stably and had a phenotype similar to NPSC, as indicated by the immunoexpression of follicle stimulating hormone receptor (FSHR), and mRNA expression of androgen receptor (AR), and Wilms' tumor antigen (WT1). Interestingly, NPSC and iPS expressed mRNA of complement regulatory proteins (CRP) such as membrane cofactor protein (CD46), decay accelerating factor (DAF or CD55), and protectin (CD59), but CD59 mRNA expression was negligible in iPS.
  
  
  CONCLUSIONS
  These results suggest that iPS, immortalized by the introduction of SV40 T, retain their original characteristics, except for the relatively low expression of CD59, and that they may be useful for future in vitro and in vivo studies of immune privilege mechanisms related to SC.
authors: Department of Thoracic and Cardiovascular Surgery, Seoul National University, Seoul, Korea.
authors: Hak-Mo
authors: HM
authors: Lee
authors: Byoung Chol
authors: BC
authors: Oh
authors: Dong-Pyo
authors: DP
authors: Lim
authors: Dong-Sup
authors: DS
authors: Lee
authors: Jaejin
authors: J
authors: Cho
authors: Gene
authors: G
authors: Lee
authors: Jeong Ryul
authors: JR
authors: Lee
mesh_terms: D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D002460:Cell Line; D049109:Cell Proliferation; D017690:Cell Transplantation / Q000379:methods*; D002478:Cells, Cultured; D003165:Complement System Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism; D011962:Receptors, FSH / Q000235:genetics / Q000378:metabolism; D012708:Sertoli Cells / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D013552:Swine; D014162:Transfection; D014183:Transplantation, Heterologous / Q000276:immunology / Q000379:methods*; D025721:WT1 Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D000952:Antigens, Polyomavirus Transforming; D012333:RNA, Messenger; D011944:Receptors, Androgen; D011962:Receptors, FSH; D025721:WT1 Proteins; D003165:Complement System Proteins