title: A new look towards BAC-based array CGH through a comprehensive comparison with oligo-based array CGH.
abstract: BACKGROUND
  Currently, two main technologies are used for screening of DNA copy number; the BAC (Bacterial Artificial Chromosome) and the recently developed oligonucleotide-based CGH (Chromosomal Comparative Genomic Hybridization) arrays which are capable of detecting small genomic regions with amplification or deletion. The correlation as well as the discriminative power of these platforms has never been compared statistically on a significant set of human patient samples.
  
  
  RESULTS
  In this paper, we present an exhaustive comparison between the two CGH platforms, undertaken at two independent sites using the same batch of DNA from 19 advanced prostate cancers. The comparison was performed directly on the raw data and a significant correlation was found between the two platforms. The correlation was greatly improved when the data were averaged over large chromosomic regions using a segmentation algorithm. In addition, this analysis has enabled the development of a statistical model to discriminate BAC outliers that might indicate microevents. These microevents were validated by the oligo platform results.
  
  
  CONCLUSIONS
  This article presents a genome-wide statistical validation of the oligo array platform on a large set of patient samples and demonstrates statistically its superiority over the BAC platform for the Identification of chromosomic events. Taking advantage of a large set of human samples treated by the two technologies, a statistical model has been developed to show that the BAC platform could also detect microevents.
authors: Laboratoire de Bioinformatique et de Génomique lntégratives, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch CEDEX, France. wicker@igbmc.u-strasbg.fr
authors: Nicolas
authors: N
authors: Wicker
authors: Annaïck
authors: A
authors: Carles
authors: Ian G
authors: IG
authors: Mills
authors: Maija
authors: M
authors: Wolf
authors: Abhi
authors: A
authors: Veerakumarasivam
authors: Henrik
authors: H
authors: Edgren
authors: Fabrice
authors: F
authors: Boileau
authors: Bohdan
authors: B
authors: Wasylyk
authors: Jack A
authors: JA
authors: Schalken
authors: David E
authors: DE
authors: Neal
authors: Olli
authors: O
authors: Kallioniemi
authors: Olivier
authors: O
authors: Poch
mesh_terms: D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D018628:Gene Dosage / Q000235:genetics*; D006801:Humans; D008297:Male; D015233:Models, Statistical; D009693:Nucleic Acid Hybridization / Q000379:methods*; D009841:Oligonucleotides / Q000235:genetics; D011471:Prostatic Neoplasms / Q000235:genetics*
chemical_list: D009841:Oligonucleotides