Self-supporting hydrogel stamps for the microcontact printing of proteins.
In this work we explore a new hydrogel stamp material obtained from polymerizing 2-hydroxyethyl acrylate and poly(ethylene glycol) diacrylate in the presence of water for the microcontact printing of proteins directly on gold substrates and by covalent coupling to self-assembled monolayers of alkanethiols. At high cross-link density, the hydrogel is rigid, hydrophilic, and with a high buffer holding capacity to enable the unsupported printing of protein patterns homogeneously and reproducibly, with micrometer-range precision. The stamps were used to print antibodies to human parathyroid hormone, which were shown using immunoassay tests to retain their biological function with binding capacities comparable to those of solution-adsorbed antibodies.
Coq
Naïs
N
Philips Research, High Tech Campus, Eindhoven, The Netherlands.
van Bommel
Ties
T
Hikmet
Rifat A
RA
Stapert
Hendrik R
HR
Dittmer
Wendy U
WU
D000179:Acrylates / Q000138:chemical synthesis / Q000737:chemistry*; D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D006046:Gold / Q000737:chemistry; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000138:chemical synthesis / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D010281:Parathyroid Hormone / Q000737:chemistry* / Q000276:immunology; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000276:immunology; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013499:Surface Properties; D014867:Water / Q000737:chemistry
D000179:Acrylates; D000906:Antibodies; D010281:Parathyroid Hormone; D013438:Sulfhydryl Compounds; C437167:poly(ethylene glycol)diacrylate; D014867:Water; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; C035957:2-hydroxyethyl acrylate; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D006046:Gold