A gene signature of 8 genes could identify the risk of recurrence and progression in Dukes' B colon cancer patients.
The benefit of postoperative adjuvant chemotherapy in patients with Dukes' B colorectal cancer is still uncertain and its routine use is not recommended. The five-year relapse rate is approximately 25-40% and the identification of patients at high risk of recurrence would represent an important strategy for the use of adjuvant chemotherapy. We retrospectively analyzed gene expression profiles in frozen tumor specimens from patients with Dukes' B colorectal cancer by using high density oligonucleotide microarrays. Our results show a subset of 48 genes differentially expressed with an associated probability <0.001 in the t-test. Another statistical procedure based on the Fisher criterion resulted in 11 genes able to separate both groups. We selected the 8 genes present in both subsets. The differential expression of five genes (CHD2, RPS5, ZNF148, BRI3 and MGC23401) in colon cancer progression was confirmed by real-time PCR in an independent set of patients of Dukes' B and C stages.
Bandrés
Eva
E
Laboratory of Pharmacogenomics, Center for Applied Medical Research, University of Navarra, 31008 Pamplona, Spain. ebandres@unav.es
Malumbres
Raquel
R
Cubedo
Elena
E
Honorato
Beatriz
B
Zarate
Ruth
R
Labarga
Alberto
A
Gabisu
Unai
U
Sola
Jesus Javier
JJ
García-Foncillas
Jesus
J
D000368:Aged; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology*; D018450:Disease Progression; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008875:Middle Aged; D005810:Multigene Family; D009364:Neoplasm Recurrence, Local / Q000235:genetics* / Q000473:pathology; D009367:Neoplasm Staging; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction / Q000379:methods; D012189:Retrospective Studies