title: Identification of a putative azadirachtin-binding complex from Drosophila Kc167 cells.
abstract: Cell-proliferation in Drosophila Kc167 cells was inhibited by 50% when cell cultures contained 1.7 x 10(-7) M azadirachtin for 48 h (a tertranortriterpenoid from the neem tree Azadirachta indica). Drosophila Kc167 cells exhibited direct nuclear damage within 6-h exposure to azadirachtin (5 x 10(-7) M and above) or within 24 h when lower concentrations were used (1 x 10(-9) M). Fractionation of an extract of Drosophila Kc167 cells combined with ligand overlay technique resulted in the identification of a putative azadirachtin binding complex. Identification of the members of this complex by Peptide Mass Fingerprinting (PMF) and N-terminal sequencing identified heat shock protein 60 (hsp60) as one of its components.
authors: School of Biological Sciences, Department of Zoology, University of Aberdeen, Aberdeen, United Kingdom.
authors: Susan L
authors: SL
authors: Robertson
authors: Weiting
authors: W
authors: Ni
authors: Tarlochan S
authors: TS
authors: Dhadialla
authors: Alasdair J
authors: AJ
authors: Nisbet
authors: Catherine
authors: C
authors: McCusker
authors: Steven V
authors: SV
authors: Ley
authors: William
authors: W
authors: Mordue
authors: A Jennifer
authors: AJ
authors: Mordue 'Luntz'
mesh_terms: D000818:Animals; D002457:Cell Extracts / Q000737:chemistry; D002460:Cell Line; D018834:Chaperonin 60 / Q000235:genetics* / Q000378:metabolism; D002850:Chromatography, Gel; D020552:Comet Assay; D019295:Computational Biology; D004249:DNA Damage; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics*; D007306:Insecticides / Q000378:metabolism / Q000633:toxicity*; D036701:Limonins / Q000378:metabolism / Q000633:toxicity*; D046912:Multiprotein Complexes / Q000235:genetics* / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D018709:Statistics, Nonparametric; D014316:Tritium
chemical_list: D002457:Cell Extracts; D018834:Chaperonin 60; D007306:Insecticides; D036701:Limonins; D046912:Multiprotein Complexes; D014316:Tritium; C010329:azadirachtin