title: Antimicrobial effect of chitooligosaccharides produced by chitosanase from Pseudomonas CUY8.
abstract: The aim of this study was to investigate the antimicrobial effect of chitooligosaccharides prepared by chitosanase from Pseudomonas CUY8. Antimicrobial activities of different degrees of deacetylation (DD) and polymerization (DP) of chitooligosaccharides against various species of bacteria and fungi were measured. The antimicrobial effects of chitooligosaccharides compared with chitosan and chitosanase were evaluated. Inhibitory diameter of chitooligosaccharides at the concentration of 0.1% with DP 4 was 19 +/- 0.20mm, and inhibitory activity with DD 90% was 79+/- 2.1%, which were higher than other DP and DD, respectively. The results showed that antimicrobial activities of chitooligosaccharides increased with increase of DD, but decreased with increase of DP. Chitooligosaccharides, chitosan and chitosanase all showed significantly stronger antimicrobial activities against bacteria than fungi (p<0.001). Antimicrobial activities of chitooligosaccharides were significantly higher than that of chitosan (p<0.05), but insignificantly lower than that of chitosanase (p>0.05).
authors: Department of Food Science, Shanghai Fisheries University, 334 Jungong Road, Shanghai, China 200090. ywang2@shfu.edu.cn
authors: Yan
authors: Y
authors: Wang
authors: Peigen
authors: P
authors: Zhou
authors: Jianxing
authors: J
authors: Yu
authors: Xiaorong
authors: X
authors: Pan
authors: Pingping
authors: P
authors: Wang
authors: Weiqing
authors: W
authors: Lan
authors: Shendan
authors: S
authors: Tao
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development; D048271:Chitosan / Q000494:pharmacology*; D015169:Colony Count, Microbial; D004305:Dose-Response Relationship, Drug; D005658:Fungi / Q000187:drug effects / Q000254:growth & development; D006026:Glycoside Hydrolases / Q000378:metabolism*; D007218:Industrial Microbiology; D008826:Microbial Sensitivity Tests; D009844:Oligosaccharides / Q000494:pharmacology*; D011549:Pseudomonas / Q000201:enzymology* / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents; D009844:Oligosaccharides; D048271:Chitosan; D006026:Glycoside Hydrolases; C019868:chitosanase