A kinetic method amenable to automation for ceruloplasmin estimation with inexpensive and stable reagents.
OBJECTIVE
To develop a kinetic, inexpensive, automatable assay for ceruloplasmin as ferroxidase.


METHODS
Dithiothreitol has been used to stabilize the substrate and quinolones to complex the ferric.


RESULTS
Mean ferroxidase activity in healthy adults, Wilson's disease and pregnancy was 787.29, 178.5 and 1828.09 IU/L, respectively. Correlation coefficient of ferroxidase vis-à-vis copper, ferroxidase by ferrozine and ceruloplasmin were 0.90, 0.94 and 0.92, respectively.


CONCLUSIONS
Norfloxacin method is a simple, inexpensive and automatable kinetic assay.
Somani
Babu Lal
BL
Department of Biochemistry, Armed Forces Medical College, Pune-411040, India. blsomani@gmail.com
Ambade
Vivek
V
D000328:Adult; D002570:Ceruloplasmin / Q000032:analysis* / Q000378:metabolism*; D019963:Clinical Chemistry Tests / Q000191:economics / Q000379:methods*; D004229:Dithiothreitol / Q000737:chemistry; D005260:Female; D005297:Ferrozine / Q000737:chemistry; D006527:Hepatolenticular Degeneration / Q000201:enzymology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D009643:Norfloxacin / Q000737:chemistry; D011247:Pregnancy; D015363:Quinolones / Q000737:chemistry; D015203:Reproducibility of Results
D015363:Quinolones; D005297:Ferrozine; D002570:Ceruloplasmin; D009643:Norfloxacin; D004229:Dithiothreitol