Postnatal biochemical changes in rat lens: an important factor in cataract models.
OBJECTIVE
Administration of several cataractogenic agents is effective during the first 21 days of rat lens development, a period of the highest sensitivity of the tissue. Thus, cataract formation and lens maturation affect the biochemical profile of rat lens simultaneously and might be difficult to evaluate separately. The purpose of this study was to use high-resolution magic angle spinning proton nuclear magnetic resonance (HR-MAS 1H NMR) to investigate exclusively the effect of maturation on the metabolic profile of rat lens.


METHODS
Albino Sprague-Dawley rats (n = 15) were divided into five groups of three animals and sacrificed at designated times (7, 14, 20, 30, and 60 days). The lenses were dissected, frozen, and thereafter analyzed with HR-MAS 1H NMR spectroscopy. Special grouping patterns among the tissue samples of different age and the relative percentage changes in particular metabolite concentrations were evaluated using appropriate statistical methods (principal components, one-way ANOVA).


RESULTS
Time-dependent alterations in the metabolic profile of the rat lens tissue were dominated by a significant increase in taurine, hypo-taurine, and myoinositol concentrations at the age of 30 days. Contents of the energy metabolites and amino acids were nearly constant between the ages of 14 and 30 days, showing a significant decrease in the 60-day-old rat lenses.


CONCLUSIONS
HR-MAS 1H NMR spectroscopy showed its suitability to assess the natural alterations in the metabolic profile of maturing rat lens. The results can be used in future cataract research designed to evaluate the metabolic effect of different cataractogenic agents during this postnatal period.
Fris
Miroslav
M
Department of Neuroscience, Faculty of Medicine, Norwegian University of Science and Technology (NTNU), 7489 Trondheim, Norway. miroslav.fris@ntnu.no
Midelfart
Anna
A
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D002386:Cataract / Q000378:metabolism*; D003459:Crystallins / Q000378:metabolism; D004195:Disease Models, Animal; D004734:Energy Metabolism; D007294:Inositol / Q000378:metabolism*; D007908:Lens, Crystalline / Q000254:growth & development* / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D009929:Organ Size; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D000596:Amino Acids; D003459:Crystallins; D007294:Inositol