Analysis of interaction property of bioactive components in Danggui Buxue Decoction with protein by microdialysis coupled with HPLC-DAD-MS.
Interaction of a commonly used combined prescription of Danggui Buxue Decoction (CPDBD) with protein was studied by microdialysis coupled with HPLC-DAD-MS. Nine compounds in CPDBD were unequivocally identified by comparing with their t(R), MS data and UV spectra with those of reference compounds, and simultaneously quantified. Microdialysis recoveries and binding degrees of 20 compounds in CPDBD with bovine serum albumin (BSA) were determined. Recoveries of microdialysis sampling ranged from 66.9 to 91.5% with RSD below 3.0%, and the binding degrees of those to BSA ranged from 6.3 to 59.8% (0.3mM BSA) and from 6.9 to 86.6% (0.6mM BSA). The results were determined at pH 7.4 and the influence of different pH value was investigated. Furthermore, the binding degrees of eight reference compounds were determined separately under the same conditions, indicating a significant effect of the interaction of compounds with each other on their binding degrees to BSA. By comparing their binding degrees with BSA with those of proven active compounds in CPDBD, i.e. chlorogenic acid (3), ferulic acid (6), ononin (12) and calycosin (16), other five compounds were found to possess potential activities, which were tentatively identified as calycosin-7-O-beta-D-glucoside-6-O-malonate (9), senkyunolide I or H (10), formononetin-7-O-beta-D-glucoside-6-O-malonate (17), and two unknown compounds.
Wen
Xiao-Dong
XD
Key Laboratory of Modern Chinese Medicines, Ministry of Education and Department of Pharmacognosy, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing 210009, China.
Qi
Lian-Wen
LW
Chen
Jun
J
Song
Yue
Y
Yi
Ling
L
Yang
Xiao-Wei
XW
Li
Ping
P
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry / Q000379:methods*; D017551:Microdialysis; D010936:Plant Extracts / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results
D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; C477279:danggui buxue decoction