Role of the FAD-dependent polyamine oxidase in the selective formation of N(1),N(8)-bis(gamma-glutamyl)spermidine protein cross-links(1).
Protein-bound gamma-glutamylpolyamines have highlighted a new pathway in polyamine metabolism. Human foreskin keratinocytes offer a suitable model for this study. Indeed, they develop polymerized envelopes, as they differentiate, rich in epsilon-(gamma-glutamyl)lysine and N(1),N(8)-bis(gamma-glutamyl)spermidine cross-links. We have found that the selective oxidation of N(1)-(gamma-glutamyl)spermidine and N-(gamma-glutamyl)spermine by FAD-dependent polyamine oxidase (PAO) may be one of the cellular mechanisms regulating the preferential formation of a sterically defined bis(gamma-glutamyl)spermidine cross-link. The significance of this finding is unknown, but it suggests that the target of this PAO-modulation is to achieve the biochemical prerequisite for production of a normal epidermal stratum corneum.
Lentini
A
A
Department of Biology, University of Rome Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy.
Mattioli
P
P
Provenzano
B
B
Abbruzzese
A
A
Caraglia
M
M
Beninati
S
S
D000818:Animals; D002454:Cell Differentiation; D003432:Cross-Linking Reagents; D019281:Dimerization; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism*; D005973:Glutamine; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000378:metabolism*; D013095:Spermidine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D011503:Transglutaminases / Q000378:metabolism*; D000097667:Polyamine Oxidase
D003432:Cross-Linking Reagents; D005182:Flavin-Adenine Dinucleotide; D005973:Glutamine; D000587:Oxidoreductases Acting on CH-NH Group Donors; D013095:Spermidine; D011503:Transglutaminases; D000097667:Polyamine Oxidase; C055363:N(1)-(gamma-glutamyl)spermidine; C058907:N(1),N(8)-bis(gamma-glutamyl)spermidine