title: Molecular characterization of angiomotin/JEAP family proteins: interaction with MUPP1/Patj and their endogenous properties.
abstract: We have previously shown that MUPP1, which has an MRE domain and 13 PDZ domains, is expressed in epithelial cells and localize at tight junctions (TJs) and apical membranes. Using yeast two-hybrid screening, we found here that MUPP1 interacts with angiomotin (Amot), JEAP/Amot-like 1 and MASCOT/Amot-like 2, which we refer to as Amot/JEAP family proteins. PDZ2 and -3 were responsible for MUPP1's interaction with Amot and MASCOT, whereas only PDZ3 was responsible for its interaction with JEAP. All the Amot/JEAP family proteins also interacted with Patj, a close relative of MUPP1. The C-terminal PDZ-binding motives of the Amot/JEAP family were required for these interactions. We successfully generated specific antibodies for these proteins and analyzed the endogenous molecular properties of the family in parallel. Immunofluorescence microscopy of cultured epithelial cells showed that in subcellular distribution, the Amot/JEAP family proteins were indistinguishable; they were apparent at TJs as well as apical membranes, and mostly co-localized with MUPP1. They were also located at TJs in several mouse tissues, but each protein showed a distinct tissue distribution. In biochemical fractionation assays, the Amot/JEAP family behaved not as transmembrane but as peripheral membrane proteins. Unexpectedly, the PDZ-binding motives were not necessarily required for their localization to TJs, and dominant negative MUPP1 or Patj did not affect the localization of Amot/JEAP family proteins, suggesting that the interaction with MUPP1/Patj is not necessarily responsible for their proper subcellular distribution.
authors.affiliation: Department of Cell Biology, Kyoto University, Yoshida-konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Sugihara-Mizuno
authors.forename: Makoto
authors.initials: M
authors.lastname: Adachi
authors.forename: Yuka
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Yoko
authors.initials: Y
authors.lastname: Hamazaki
authors.forename: Miyuki
authors.initials: M
authors.lastname: Nishimura
authors.forename: Toshio
authors.initials: T
authors.lastname: Imai
authors.forename: Mikio
authors.initials: M
authors.lastname: Furuse
authors.forename: Shoichiro
authors.initials: S
authors.lastname: Tsukita
mesh_terms: D000091082:Angiomotins; D000091304:Angiopoietin-Like Protein 1; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D062793:Occludin; D011485:Protein Binding; D019108:Tight Junctions / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: C511047:Amot protein, mouse; C449807:Amotl1 protein, mouse; D000091082:Angiomotins; D000091304:Angiopoietin-Like Protein 1; D002352:Carrier Proteins; D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D008840:Microfilament Proteins; C491750:Mpdz protein, mouse; C568985:OCLN protein, human; D062793:Occludin; C568986:Ocln protein, mouse