title: Control of sigma virus multiplication by the ref(2)P gene of Drosophila melanogaster: an in vivo study of the PB1 domain of Ref(2)P.
abstract: Ref(2)P has been described as one of the Drosophila proteins that interacts with the sigma virus cycle. We generated alleles to identify critical residues involved in the restrictive (inhibiting viral multiplication) or permissive (allowing viral multiplication) character of Ref(2)P. We demonstrate that permissive alleles increase the ability of the sigma virus to infect Drosophila when compared to null alleles and we confirm that restrictive alleles decrease this capacity. Moreover, we have created alleles unfunctional in viral cycling while functional for Ref(2)P fly functions. This type of allele had never been observed before and shows that fly- and virus-related activities of Ref(2)P are separable. The viral status of Ref(2)P variants is determined by the amino-terminal PB1 domain polymorphism. In addition, an isolated PB1 domain mimics virus-related functions even if it is similar to a loss of function toward fly-related activities. The evolutionary tree of the Ref(2)P PB1 domain that we could build on the basis of the natural allele sequences is in agreement with an evolution of PB1 domain due to successive transient selection waves.
authors.affiliation: Université Versailles SQY, CNRS, Laboratoire de Génétique et Biologie Cellulaire-UMR 8159, 78035 Versailles, France.
authors.forename: A
authors.initials: A
authors.lastname: Carré-Mlouka
authors.forename: S
authors.initials: S
authors.lastname: Gaumer
authors.forename: P
authors.initials: P
authors.lastname: Gay
authors.forename: A M
authors.initials: AM
authors.lastname: Petitjean
authors.forename: C
authors.initials: C
authors.lastname: Coulondre
authors.forename: P
authors.initials: P
authors.lastname: Dru
authors.forename: F
authors.initials: F
authors.lastname: Bras
authors.forename: S
authors.initials: S
authors.lastname: Dezélée
authors.forename: D
authors.initials: D
authors.lastname: Contamine
mesh_terms: D000483:Alleles; D000818:Animals; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins / Q000737:chemistry* / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000821:virology*; D019143:Evolution, Molecular; D017344:Genes, Insect; D005838:Genotype; D009154:Mutation / Q000235:genetics; D009687:Nuclear Proteins / Q000737:chemistry* / Q000235:genetics*; D011110:Polymorphism, Genetic; D017434:Protein Structure, Tertiary; D012209:Rhabdoviridae / Q000502:physiology*; D018353:Rhabdoviridae Infections; D019076:Transgenes; D014779:Virus Replication
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D009687:Nuclear Proteins; C080596:ref(2)P protein, Drosophila