title: [Radiosensitivity of nasopharyngeal carcinoma in relation to cell cycle synchronization effect of tumor necrosis factor alpha].
abstract: OBJECTIVE
  To investigate the effect of tumor necrosis factor alpha (TNFalpha) on radiosensitivity of nasopharyngeal carcinoma (NPC) in relation to TNFalpha-induced cell cycle synchronization.
  
  
  METHODS
  The radio-resistance of a NPC cell line subclone CNE-2Z-S1 was verified by in vivo experiments and flow cytometry was performed to evaluate cell cycle synchronization in TNFalpha-treated CNE-2Z-S1 cells. The radiosensitivity of the cell synchronized CNE-2Z-S1 cells was determined by clone formation in vitro and in vivo experiment in nude mice.
  
  
  RESULTS
  TNFalpha was capable of inducing cell cycle arrest and synchronization of CNE-2Z-S1 cells. Pretreatment with TNFalpha remarkably enhanced the radiosensitivity of CNE-2Z-S1 in vitro, and in vivo experiments with nude mice also suggested the role of TNFalpha in enhancing the radiosensitivity of NPC.
  
  
  CONCLUSIONS
  TNFalpha can enhance the radiosensitivity of NPC cells by inducing cell cycle synchronization.
authors.affiliation: Department of Pathology, Guangdong Pharmacy College, Guangzhou 510224, China. janeyuan9909@sina.com
authors.forename: Jian
authors.initials: J
authors.lastname: Yuan
authors.forename: Ning
authors.initials: N
authors.lastname: Sun
authors.forename: Li-jing
authors.initials: LJ
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Tian
authors.forename: Xiao-ming
authors.initials: XM
authors.lastname: Zhou
authors.forename: Dong-bo
authors.initials: DB
authors.lastname: Chen
authors.forename: Hong-biao
authors.initials: HB
authors.lastname: Gu
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000187:drug effects* / Q000528:radiation effects; D045744:Cell Line, Tumor; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009303:Nasopharyngeal Neoplasms / Q000188:drug therapy* / Q000473:pathology / Q000532:radiotherapy; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D023041:Xenograft Model Antitumor Assays
chemical_list: D011838:Radiation-Sensitizing Agents; D014409:Tumor Necrosis Factor-alpha