HPLC determination and pharmacokinetic study of homoeriodictyol-7-O-beta-D-glucopyranoside in rat plasma and tissues.
Homoeriodictyol-7-O-beta-D-glucopyranoside (HEDT-Glu) was isolated from Viscum coloratum and identified by MS, 1H- and 13C-NMR. A HPLC method was developed for determination of HEDT-Glu in rat plasma and tissues. All biological samples were pretreated by protein precipitation with acetone. Vanillin was selected as internal standard. The mobile phase consisted of methanol-water-glacial acetic acid (45 : 55 : 0.5, v/v/v). Good linearity were observed over the concentration ranges of 0.1-200.0 microg.ml(-1) in rat plasma and 0.05-5.0 microg.ml(-1) in tissues. Both intra- and inter-day precisions of HEDT-Glu, expressed as the relative standard deviation, were less than 13.1%. Accuracy, expressed as the relative error, ranged from -0.8 to 5.4% in plasma and from -5.6 to 9.4% in tissues. The mean extraction recovery of HEDT-Glu was above 73.17% in biological samples. The described assay method was successfully applied to the pre-clinical pharmacokinetic study of HEDT-Glu. After intravenous administration of HEDT-Glu to rat, AUC and CL(tot) were 16.04+/-3.19 microg.h.ml(-1) and 0.85+/-0.17 l.kg(-1).h(-1), respectively. T(1/2,alpha) and t(1/2,beta) were 0.06+/-0.01 h and 1.27+/-0.31 h, respectively. HEDT-Glu was cleared from the blood and mainly distributed to the liver and small intestine.
Zhao
Yunli
Y
School of Pharmacy, Shenyang Pharmaceutical University, P.R. China.
Wang
Xiaoying
X
Zhao
Yingchun
Y
Gao
Xiaoxia
X
Bi
Kaishun
K
Yu
Zhiguo
Z
D000818:Animals; D019540:Area Under Curve; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D005419:Flavonoids / Q000032:analysis* / Q000302:isolation & purification / Q000493:pharmacokinetics*; D006027:Glycosides / Q000032:analysis* / Q000302:isolation & purification / Q000493:pharmacokinetics*; D007421:Intestine, Small / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008297:Male; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013047:Specific Pathogen-Free Organisms; D014018:Tissue Distribution; D028181:Viscum / Q000737:chemistry
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D006027:Glycosides; C519678:homoeriodictyol-7-O-beta-D-glucopyranoside