Coordination of three and four Cu(I) to the alpha- and beta-domain of vertebrate Zn-metallothionein-1, respectively, induces significant structural changes.
Vertebrate metallothioneins are found to contain Zn(II) and variable amounts of Cu(I), in vivo, and are believed to be important for d10-metal control. To date, structural information is available for the Zn(II) and Cd(II) forms, but not for the Cu(I) or mixed metal forms. Cu(I) binding to metallothionein-1 has been investigated by circular dichroism, luminescence and 1H NMR using two synthetic fragments representing the alpha- and the beta-domain. The 1H NMR data and thus the structures of Zn4alpha metallothionein (MT)-1 and Zn3betaMT-1 were essentially the same as those already published for the corresponding domains of native Cd7MT-1. Cu(I) titration of the Zn(II)-reconstituted domains provided clear evidence of stable polypeptide folds of the three Cu(I)-containing alpha- and the four Cu(I)-containing beta-domains. The solution structures of these two species are grossly different from the structures of the starting Zn(II) complexes. Further addition of Cu(I) to the two single domains led to the loss of defined domain structures. Upon mixing of the separately prepared aqueous three and four Cu(I) loaded alpha- and beta-domains, no interaction was seen between the two species. There was neither any indication for a net transfer of Cu(I) between the two domains nor for the formation of one large single Cu(I) cluster involving both domains.
Dolderer
Benedikt
B
Interfakultäres Institut für Biochemie, University of Tübingen, Germany.
Echner
Hartmut
H
Beck
Alexander
A
Hartmann
Hans-Jürgen
HJ
Weser
Ulrich
U
Luchinat
Claudio
C
Del Bianco
Cristina
C
D000595:Amino Acid Sequence; D000818:Animals; D003300:Copper / Q000737:chemistry*; D008668:Metallothionein / Q000737:chemistry*; D051379:Mice; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D017434:Protein Structure, Tertiary; D012996:Solutions; D013329:Structure-Activity Relationship; D017186:Titrimetry; D015032:Zinc / Q000737:chemistry*
D012996:Solutions; C407523:metallothionein isoform 1; C021267:zinc thionein; D003300:Copper; D008668:Metallothionein; D015032:Zinc