title: Histamine H1 and H2 receptors but not H4 receptors are upregulated during bone marrow regeneration.
abstract: The role of histamine receptors in radiation-induced bone marrow (BM) regeneration was investigated with aspects of functional genomics. H1R and H2R mRNA expression increased during regeneration in both histidine decarboxylase knockout (HDC-/-) and wild type (HDC+/+) mice, though to a lesser extent in HDC-/- mice. H4R mRNA expression was downregulated in both groups. Mainly CD34+ cells were responsible for the elevation of intracellular histamine and HDC content in HDC+/+ BM cell populations. The differential changes in the expression of its receptors, and also its elevated levels in hematopoietic progenitors support the regulatory role of histamine in BM regeneration, that could be further explored by future gene expression studies.
authors.affiliation: Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Horváth
authors.forename: Eva
authors.initials: E
authors.lastname: Pállinger
authors.forename: Gyozo
authors.initials: G
authors.lastname: Horváth
authors.forename: Ivett
authors.initials: I
authors.lastname: Jelinek
authors.forename: András
authors.initials: A
authors.lastname: Falus
authors.forename: Edit I
authors.initials: EI
authors.lastname: Buzás
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000502:physiology*; D005434:Flow Cytometry; D006410:Hematopoiesis / Q000502:physiology; D006640:Histidine Decarboxylase / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis* / Q000235:genetics; D011968:Receptors, Histamine / Q000096:biosynthesis* / Q000235:genetics; D011969:Receptors, Histamine H1 / Q000096:biosynthesis* / Q000235:genetics; D011970:Receptors, Histamine H2 / Q000096:biosynthesis* / Q000235:genetics; D000074040:Receptors, Histamine H4; D012038:Regeneration / Q000235:genetics / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: C498829:Hrh4 protein, mouse; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; D011968:Receptors, Histamine; D011969:Receptors, Histamine H1; D011970:Receptors, Histamine H2; D000074040:Receptors, Histamine H4; D006640:Histidine Decarboxylase