Effect of synthetic hydroxy isothiocyanates on a bacterial virus and DNA.
The Effect of hydroxy isothiocyanates on a bacterial virus and M13 DNA was examined. Hydroxy-substituted phenyl and phenyl alkyl isothiocyanates, especially 2-(3,4-dihydroxyphenyl)ethyl isothiocyanate(IT-Dop) synthesized from dopamine, showed antiviral activity on psiK. In transfection experiments with M13 mp DNA species, IT-Dop inhibited the single-stranded (SS) molecule more effectively than the double stranded replicative form (RF) DNA. These effects were dependent on reaction time, and on IT-Dop concentration. An additional experiment indicated that treatment with IT-Dop suppressed annealing (reassociation) of denatured DNA. These results indicate that IT-Dop reacts mildly with virus and SS DNA.
Tajima
Hirokuni
H
Division of Material Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa, Japan. hi-tajima@rengo.co.jp
Nakamoto
Yoshiaki
Y
Taketo
Akira
A
D000998:Antiviral Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D017104:Bacteriophage M13 / Q000187:drug effects* / Q000528:radiation effects; D004261:DNA Replication / Q000187:drug effects; D004269:DNA, Bacterial / Q000737:chemistry; D004279:DNA, Viral / Q000187:drug effects* / Q000528:radiation effects; D004926:Escherichia coli / Q000737:chemistry; D006900:Hydroxylation; D017879:Isothiocyanates / Q000138:chemical synthesis / Q000494:pharmacology*; D007700:Kinetics; D009691:Nucleic Acid Denaturation; D013329:Structure-Activity Relationship; D014466:Ultraviolet Rays
D000998:Antiviral Agents; D004269:DNA, Bacterial; D004279:DNA, Viral; D017879:Isothiocyanates