[Expression of Langerhans cell-related chemokine genes in condyloma acuminatum epidermis].
OBJECTIVE
To investigate differential gene expression of Langerhans cell-related chemokines in condyloma acuminatum (CA) epidermis and normal epidermis.


METHODS
Gene expression of Langerhans cell-related chemokines in three CA epidermal lesions and in three normal controls was screened using Affymetrix oligonucleotide microarrays HG-U 133A 2.0, and part of the above differential gene expression was confirmed by semi-quantitative RT-PCR.


RESULTS
With microarrays, seven down-regulated genes of Langerhans cell-related chemokine were detected in three CA epidermal lesions as compared with three normal controls, and the down-regulation of CXCR4 and CCL20 was verified by semi-quantitative RT-PCR.


CONCLUSIONS
Several Langerhans cell-related chemokine genes are found down-regulated in CA epidermis as compared with normal epidermis, and the down-regulation of these genes may contribute to the decreased number and the homing disturbance of LC in CA epidermis.
Feng
Jin-Yun
JY
Department of Dermatology, the Second Hospital, Xi'an Jiaotong University, Xi'an 710004, China. fjyfjy888@163.com
Peng
Zhen-Hui
ZH
Rao
Guo-Zhou
GZ
Tang
Xiao-Ping
XP
Liu
Yu-Ping
YP
D000293:Adolescent; D000328:Adult; D054418:Chemokine CCL20 / Q000235:genetics; D018925:Chemokines / Q000235:genetics*; D003218:Condylomata Acuminata / Q000503:physiopathology*; D004817:Epidermis / Q000378:metabolism* / Q000821:virology; D005786:Gene Expression Regulation; D006801:Humans; D007801:Langerhans Cells / Q000378:metabolism* / Q000821:virology; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D010409:Penile Diseases / Q000503:physiopathology* / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
D054418:Chemokine CCL20; D018925:Chemokines