Effects of the monomeric, oligomeric, and fibrillar Abeta42 peptides on the proliferation and differentiation of adult neural stem cells from subventricular zone.
The incidence of amyloid plaques, composed mainly of beta-amyloid peptides (Abeta), does not correlate well with the severity of neurodegeneration in patients with Alzheimer's disease (AD). The effects of Abeta(42) on neurons or neural stem cells (NSCs) in terms of the aggregated form remain controversial. We prepared three forms of oligomeric, fibrillar, and monomeric Abeta(42) peptides and investigated their effects on the proliferation and neural differentiation of adult NSCs, according to the degree of aggregation or concentration. A low micromolar concentration (1 micromol/L) of oligomeric Abeta(42) increased the proliferation of adult NSCs remarkably in a neurosphere assay. It also enhanced the neuronal differentiation of adult NSCs and their ability to migrate. These results provide us with valuable information regarding the effects of Abeta(42) on NSCs in the brains of patients with AD.
Heo
Chaejeong
C
Department of Pharmacology, College of Medicine, National Creative Research Initiative Center for Alzheimer's Dementia, Neuroscience Research Institute, MRC, Seoul National University, Seoul, South Korea.
Chang
Keun-A
KA
Choi
Hee Soon
HS
Kim
Hye-Sun
HS
Kim
Seonghan
S
Liew
Hyunjeong
H
Kim
Jeong A
JA
Yu
Eunah
E
Ma
Jie
J
Suh
Yoo-Hun
YH
D053687:Adult Stem Cells / Q000187:drug effects / Q000378:metabolism*; D000544:Alzheimer Disease / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D016229:Amyloid beta-Peptides / Q000378:metabolism* / Q000633:toxicity; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D020547:Lateral Ventricles / Q000166:cytology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009416:Nerve Regeneration / Q000502:physiology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism* / Q000633:toxicity; D058225:Plaque, Amyloid / Q000378:metabolism / Q000473:pathology; D011108:Polymers / Q000378:metabolism / Q000633:toxicity; D018874:Spheroids, Cellular; D013687:Telencephalon / Q000166:cytology / Q000378:metabolism
D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; D011108:Polymers; C075222:amyloid beta-protein (1-42)