Polyphenols of Rosa L. leaves extracts and their radical scavenging activity.
Antioxidant potential of Rosa L. leaves methanolic extracts was evaluated in vitro using a spectrophotometric method based on measuring the radical scavenging effect on 2,2-di-phenyl-1-picrylhydrazyl (DPPH) radicals. The contents of ellagic acid, quercetin and kaempferol in the extracts from leaves of seventeen rose species were determined using SPE-RP-HPLC methods. Additionally, total phenolic content was determined spectrophotometrically according to the Folin-Ciocalteu procedure and calculated as gallic acid equivalents (GAE). Remarkable high antioxidant activity and high total phenolic content (5.7% < GAE < 15.2%), large ellagic acid (EA) content from 9.37 to 19.42 mg/g of dry weight, a quercetin content ranging from 3.68 to 15.81 mg/g of dry weight and kaempferol content from 1.25 to 9.41mg/g of dry weight were found in rose leaves. Significant correlation between EA (r(2) = 0.6131), quercetin (r(2) = 0.5158), total phenolic content (r(2)= 0.8485) and antioxidant activity was observed. Basing on the studies conducted one may assume that the extracts of rose leaves are a rich source of natural antioxidants and could be used to prevent free-radical-induced deleterious effects.
Nowak
Renata
R
Department of Pharmaceutical Botany, Medical University of Lublin, Chodiki 1 Str., 20-093 Lublin, Poland. renata.nowak@am.lublin.pl
Gawlik-Dziki
Urszula
U
D000975:Antioxidants / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004610:Ellagic Acid / Q000378:metabolism; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D044948:Flavonols / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology*; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry*; D059808:Polyphenols; D027826:Rosa / Q000737:chemistry*; D013053:Spectrophotometry
D000975:Antioxidants; D005419:Flavonoids; D044948:Flavonols; D016166:Free Radical Scavengers; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D004610:Ellagic Acid