Fish species identification in surimi-based products.
Whole fish morphologically identified as belonging to Theragra chalcogramma, Merluccius merluccius, Merluccius hubbsi, and Merluccius capensis and 19 fish products commercialized as surimi with different commercial brands and labeled as T. chalcogramma were analyzed by direct sequence analysis of the cytochrome b gene. A phylogenetic analysis of surimi products was performed as well. Results demonstrated that mislabeling is a large-scale phenomenon, since 84.2% of surimi-based fish products sold as T. chalcogramma (16/19) were prepared with species different from the one declared. In fact, only three samples (samples 15-17) were found to belong to T. chalcogramma. In the remaining samples, Merluccidae (samples 4-14), Gadidae (samples 18 and 19), Sparidae (sample 1), and Pomacentridae (samples 2 and 3) families were detected. A phylogenetic tree was constructed, and the bootstrap value was calculated. According to this methodology, 11 samples were grouped in the same clade as Merluccius spp.
Pepe
Tiziana
T
Dipartimento di Scienze Zootecniche e Ispezione degli Alimenti, Sezione Ispezione, Falcoltà di Medicina Veterinaria, Università Federico II, Napoli, Italy.
Trotta
Michele
M
Di Marco
Isolina
I
Anastasio
Aniello
A
Bautista
José Manuel
JM
Cortesi
Maria Luisa
ML
D000818:Animals; D045303:Cytochromes b / Q000235:genetics; D004247:DNA / Q000032:analysis; D005396:Fish Products / Q000145:classification*; D005399:Fishes / Q000145:classification* / Q000235:genetics; D048388:Gadiformes / Q000145:classification / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D004247:DNA; D045303:Cytochromes b