title: Combined virus-like particle-based polyepitope DNA/protein HIV-1 vaccine design, immunogenicity and toxicity studies.
abstract: We have previously described designing of polyepitope immunogens TBI and TCI, to stimulate the humoral and cellular immune responses to HIV-1. Here, immunogens TBI and TCI were used to create new vaccine construct named CombiHIVvac (Combined HIV-1 vaccine). CombiHIVvac is a virus-like particles (VLP) containing the DNA vaccine pcDNA-TCI as a core encapsulated within a spermidine-polyglucin-TBI conjugate. The immunogenic and toxic properties of the candidate vaccine CombiHIVvac have been studied. CombiHIVvac induces a strong humoral and CTL responses in mice; the antibodies are highly specific and are able to neutralize HIV-1 in vitro. Preclinical study demonstrated that CombiHIVvac does not cause long-term changes in physiological, biochemical and morphological parameters in immunized animals and thus can be recommended for clinical trials.
authors.affiliation: State Research Center of Virology and Biotechnology Vector, 630559 Koltsovo, Novosibirsk region, Russia. karpenko@vector.nsc.ru
authors.forename: Larisa I
authors.initials: LI
authors.lastname: Karpenko
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Ilyichev
authors.forename: Alexey M
authors.initials: AM
authors.lastname: Eroshkin
authors.forename: Leonid R
authors.initials: LR
authors.lastname: Lebedev
authors.forename: Roman V
authors.initials: RV
authors.lastname: Uzhachenko
authors.forename: Nadezhda A
authors.initials: NA
authors.lastname: Nekrasova
authors.forename: Olga A
authors.initials: OA
authors.lastname: Plyasunova
authors.forename: Pavel A
authors.initials: PA
authors.lastname: Belavin
authors.forename: Sergei V
authors.initials: SV
authors.lastname: Seregin
authors.forename: Nadezhda K
authors.initials: NK
authors.lastname: Danilyuk
authors.forename: Boris N
authors.initials: BN
authors.lastname: Zaitsev
authors.forename: Elena D
authors.initials: ED
authors.lastname: Danilenko
authors.forename: Valentina I
authors.initials: VI
authors.lastname: Masycheva
authors.forename: Sergei I
authors.initials: SI
authors.lastname: Bazhan
mesh_terms: D016915:AIDS Vaccines / Q000009:adverse effects / Q000737:chemistry / Q000276:immunology*; D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D015483:HIV Antibodies / Q000097:blood; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D009500:Neutralization Tests; D019444:Vaccines, DNA / Q000009:adverse effects / Q000737:chemistry / Q000276:immunology*; D022721:Vaccines, Virosome / Q000009:adverse effects / Q000737:chemistry / Q000276:immunology*
chemical_list: D016915:AIDS Vaccines; D016207:Cytokines; D000939:Epitopes; D015483:HIV Antibodies; D019444:Vaccines, DNA; D022721:Vaccines, Virosome