Serial changes in CT cerebral blood volume and flow after 4 hours of middle cerebral occlusion in an animal model of embolic cerebral ischemia.
OBJECTIVE
Neuroimaging techniques have the potential to improve acute stroke treatment by selecting the appropriate patients for thrombolytic therapy. In this study, we examined changes in cerebral blood flow (CBF) and cerebral blood volume (CBV) in an animal model of middle cerebral artery occlusion and used these to identify the parameters that best differentiate between oligemic and infarct regions.


METHODS
Permanent middle cerebral artery occlusion was performed in 17 New Zealand white rabbits. CT perfusion imaging was performed before (baseline), 10, and 30 minutes after the stroke, and then every 30 minutes up to 3 hours. After a final scan at 4 hours, the brain was removed, cut corresponding to CT sections, and stained with 2,3,5-triphenyltetrazolium chloride (TTC) to identify infarcted tissue. A logistic regression model with the 4-hour post-CBF and -CBV values as independent variables was used to determine the binary tissue outcome variable (oligemia or infarction).


RESULTS
Infarcted regions were characterized by a significant decrease (P < .005) in both CBV and CBF, whereas oligemic (CBF < 25 mL . 100 g(-1) . min(-1), not infarcted) regions showed a significant decrease (P < .005) in CBF with maintenance of CBV at or near baseline values. From the perfusion parameters at the 4-hour time point, logistic regression by using CBV*CBF resulted in a sensitivity of 90.6% and a specificity of 93.3% for infarction.


CONCLUSIONS
CBF and CBV values obtained from CT perfusion imaging can be used to distinguish between oligemic and infarct regions. This information could be used to assess the viability of ischemic brain tissue.
Murphy
B D
BD
Robarts Research Institute, London, Ontario, Canada.
Chen
X
X
Lee
T-Y
TY
D000818:Animals; D001783:Blood Flow Velocity; D001810:Blood Volume; D001921:Brain / Q000473:pathology; D002545:Brain Ischemia / Q000000981:diagnostic imaging / Q000473:pathology / Q000503:physiopathology*; D002560:Cerebrovascular Circulation; D020244:Infarction, Middle Cerebral Artery / Q000000981:diagnostic imaging / Q000473:pathology / Q000503:physiopathology; D008297:Male; D011817:Rabbits; D012680:Sensitivity and Specificity; D020521:Stroke / Q000000981:diagnostic imaging / Q000473:pathology / Q000503:physiopathology*; D014057:Tomography, X-Ray Computed