Simultaneous fluorometric resolution of pyridoxal, pyridoxamine and 4-pyridoxic acid using chemometric methods.
An alternatively minimizing covariant matrix error (AMCME) algorithm, newly proposed by the present authors, was applied to the simultaneous fluorometric determination of pyridoxal, pyridoxamine and 4-pyridoxic acid without loss of sensitivity. The experimental results illustrate that the profiles of spectra and concentration can be accurately resolved using the AMCME algorithm with a high sensitivity and stable repeatability. That is to say, the closely overlapping problem of the spectra could be resolved owing to the characteristic features of the AMCME algorithm.
Mo
Cui-Yun
CY
Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Provincial Institute of Microbiology, Guangzhou, China.
Cao
Yu-Zhen
YZ
D000465:Algorithms; D011730:Pyridoxal / Q000032:analysis*; D011733:Pyridoxamine / Q000032:analysis*; D011735:Pyridoxic Acid / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D014803:Vitamin B Complex / Q000032:analysis*
D014803:Vitamin B Complex; D011730:Pyridoxal; D011735:Pyridoxic Acid; D011733:Pyridoxamine