title: [Transduction peptides: structural-functional analyses in model membranes].
abstract: Peptide-membrane interaction is the first step required for peptide cell internalization. In this paper we studied the interactions of substance P, Penetratin and an amphiphilic 16mer (RL16) peptide in two different model membranes, giant unilamellar vesicles and large unilamellar vesicles. Penetratin was able to induce the formation of tubes inside the giant vesicles without changes in membrane permeability. On the contrary, RL16 induced the disruption of giant vesicles and the permeabilization of large vesicles. Substance P showed none of these effects.
authors.affiliation: UMR Inserm U538, CHU Saint Antoine, Université Pierre et Marie Curie, 27 rue de Chaligny, 75012 Paris, France.
authors.forename: Antonin
authors.initials: A
authors.lastname: Lamazière
authors.forename: Gérard
authors.initials: G
authors.lastname: Chassaing
authors.forename: Germain
authors.initials: G
authors.lastname: Trugnan
authors.forename: Jesus
authors.initials: J
authors.lastname: Ayala-Sanmartin
mesh_terms: D001704:Biopolymers; D002352:Carrier Proteins / Q000502:physiology; D057846:Cell-Penetrating Peptides; D004339:Drug Compounding; D005452:Fluoresceins / Q000378:metabolism; D008081:Liposomes; D008563:Membrane Lipids; D008567:Membranes, Artificial; D010455:Peptides / Q000737:chemistry / Q000502:physiology*; D010539:Permeability; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D010940:Plant Proteins / Q000737:chemistry / Q000502:physiology*; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D013373:Substance P / Q000502:physiology
chemical_list: D001704:Biopolymers; D002352:Carrier Proteins; D057846:Cell-Penetrating Peptides; D005452:Fluoresceins; D008081:Liposomes; D008563:Membrane Lipids; D008567:Membranes, Artificial; D010455:Peptides; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D010940:Plant Proteins; C015462:polyarginine; C027819:polyleucine; D013373:Substance P; C414409:penetratin; C007740:fluorexon