Tissue culture of Cinchona pubescens: effects of media modifications on the growth.
Studies were performed on the growth of callus cultures of CINCHONA PUBESCENS V AHL concerning the influence of the concentration of major and minor elements described by M URASHIGE and S KOOG [1], as well as a number of organic constituents in the medium. After 13 weeks a maximum increase of the fresh and dry weight was observed in tissues grown on a medium containing 2,4-D (1 mg/l) and BA (2 mg/l) as growth factors and with the same or the half concentration of mineral salts as used by M URASHIGE and S KOOG.
Mulder-Krieger
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Department of Pharmacognosy, State University of Leiden, Gorlaeus Laboratories, Leiden, The Netherlands.
Verpoorte
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Baerheim Svendsen
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