A strong constitutive gene expression system derived from ibAGP1 promoter and its transit peptide.
To develop a strong constitutive gene expression system, the activities of ibAGP1 promoter and its transit peptide were investigated using transgenic Arabidopsis and a GUS reporter gene. The ibAGP1 promoter directed GUS expression in almost entire tissues including rosette leaf, inflorescence stem, inflorescence, cauline leaf and root, suggesting that the ibAGP1 promoter is a constitutive promoter. GUS expression mediated by ibAGP1 promoter was weaker than that by CaMV35S promoter in all tissue types, but when GUS protein was targeted to plastids with the aid of the ibAGP1 transit peptide, GUS levels increased to higher levels in lamina, petiole and cauline leaf compared to those produced by CaMV35S promoter. The enhancing effect of ibAGP1 transit peptide on the accumulation of foreign protein was tissue-specific; accumulation was high in lamina and inflorescence, but low in root and primary inflorescence stem. The transit peptide effect in the leaves was maintained highly regardless of developmental stages of plants. The ibAGP1 promoter and its transit peptide also directed strong GUS gene expression in transiently expressed tobacco leaves. These results suggest that the ibAGP1 promoter and its transit peptide are a strong constitutive foreign gene expression system for transgenesis of dicot plants.
Kwak
Man Sup
MS
School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, South Korea.
Oh
Mi-Joung
MJ
Lee
Shin Woo
SW
Shin
Jeong Sheop
JS
Paek
Kyung-Hee
KH
Bae
Jung Myung
JM
D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development; D001483:Base Sequence; D002734:Chlorophyll / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism; D035264:Flowers / Q000378:metabolism; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D051599:Glucose-1-Phosphate Adenylyltransferase / Q000235:genetics* / Q000378:metabolism; D027723:Ipomoea batatas / Q000235:genetics* / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012639:Seeds / Q000378:metabolism
D008565:Membrane Proteins; D010940:Plant Proteins; C045625:nodulin; D002734:Chlorophyll; D051599:Glucose-1-Phosphate Adenylyltransferase