Rapid method for evaluation of cholinesterase inhibitors.
The fluorescence intensity of reversible inhibitor ethidium bromide fluorophore complex with equine blood butyryl cholinesterase decreases in the presence of inhibitor (tacrine) not fluorescing in the visible spectrum. An express method for tacrine evaluation is developed.
Gainullina
E T
ET
Military University of Radiation, Chemical, and Biological Protection, Moscow.
Klyuster
O V
OV
Ryzhikov
S B
SB
Taranchenko
V F
VF
D000818:Animals; D002091:Butyrylcholinesterase / Q000378:metabolism; D002800:Cholinesterase Inhibitors / Q000032:analysis*; D004996:Ethidium; D006736:Horses; D013050:Spectrometry, Fluorescence / Q000379:methods; D013619:Tacrine / Q000032:analysis; D013696:Temperature
D002800:Cholinesterase Inhibitors; D013619:Tacrine; D002091:Butyrylcholinesterase; D004996:Ethidium