title: Selective separation of hydroxy polychlorinated biphenyls (HO-PCBs) by the structural recognition on the molecularly imprinted polymers: direct separation of the thyroid hormone active analogues from mixtures.
abstract: We developed novel separation media for hydroxy polychlorinated biphenyls (HO-PCBs) using the molecular imprinting techniques. The results of evaluation for the molecularly imprinted polymers (MIPs) by the liquid chromatography (LC) suggested that MIPs had selective separation ability for certain HO-PCB analogues. The results of the LC evaluations and molecular modeling indicated that the molecular volumes and pK(a) values of template molecules were related with the retention factor of HO-PCBs. Additionally, according to the detail evaluation toward the selective separation behaviors of MIPs, these HO-PCB analogues have low pK(a) values dependent on their chemical structures. In other words, the prepared MIPs had selective recognition ability against the analogues, which have an OH group on a phenyl carbon and two chlorine atoms on the both neighboring carbons of the carbon attached with the OH group. Moreover, these analogues may have a potential for thyroid hormone activities so that we attempted to separate these analogues directly from mixtures of HO-PCBs using a prepared MIP.
authors.affiliation: Graduate School of Environmental Studies, Tohoku University, Aoba 6-6-20, Aramaki, Aoba-ku, Sendai 980-8579, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Kubo
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Matsumoto
authors.forename: Fujio
authors.initials: F
authors.lastname: Shiraishi
authors.forename: Makoto
authors.initials: M
authors.lastname: Nomachi
authors.forename: Koji
authors.initials: K
authors.lastname: Nemoto
authors.forename: Ken
authors.initials: K
authors.lastname: Hosoya
authors.forename: Kunimitsu
authors.initials: K
authors.lastname: Kaya
mesh_terms: D002853:Chromatography, Liquid; D011078:Polychlorinated Biphenyls / Q000737:chemistry / Q000302:isolation & purification*; D011108:Polymers / Q000737:chemistry*; D013963:Thyroid Hormones / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D011108:Polymers; D013963:Thyroid Hormones; D011078:Polychlorinated Biphenyls