title: Immunocytochemistry on suprachiasmatic nucleus slices.
abstract: Immunocytochemistry (ICC) is a sensitive and powerful method that is used to localize and identify cells containing a particular antigen. This chapter is dedicated to ICC of suprachiasmatic nucleus (SCN) slices. After a brief introduction to the technique, the materials and methods sections describe two different methods to obtain SCN slices--the first one for fixed tissue, the second one for fresh frozen tissue--followed by the description of two methods of antibody detection: the indirect method and the avidin-biotin complex one. In addition, some remedies to the most common problems encountered while performing ICC and some alternative protocols are discussed.
authors.affiliation: Departrment of Molecular and Integrative Neuroscience, Imperial College London, UK.
authors.forename: Marta Muñoz
authors.initials: MM
authors.lastname: Llamosas
mesh_terms: D000818:Animals; D000906:Antibodies; D016616:Cryoultramicrotomy; D007150:Immunohistochemistry / Q000379:methods*; D066298:In Vitro Techniques; D013493:Suprachiasmatic Nucleus / Q000378:metabolism*; D016707:Tissue Fixation
chemical_list: D000906:Antibodies