Functionalized magnetic nanoparticles for small-molecule isolation, identification, and quantification.
Functionalized magnetic nanoparticles (MNPs) were synthesized to serve as laser desorption/ionization elements as well as solid-phase extraction probes for simultaneous enrichment and detection of small molecules in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis. Two laser-absorbing matrices were each conjugated onto MNP to give MNP@matrix which provided high ionization efficiency and background-free detection in MS leading to unambiguous identification of target small molecules in a complex mixture. MNP@matrix was demonstrated to serve as a general matrix-free additive in MALDI-TOF MS analysis of structurally distinct small molecules. Also, MNP@matrix provides a simple, rapid, and reliable quantitative assay for small molecules by mass without either the use of an internal standard or an isotopic labeling tag. Furthermore, the affinity extraction of small molecules from complex biofluid was achieved by probe protein-conjugated MNP@matrix without laborious purification. We demonstrated that a nanoprobe-based assay is a cost-effective, rapid, and accurate platform for robotic screening of small molecules.
Lin
Po-Chiao
PC
Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan.
Tseng
Mei-Chun
MC
Su
An-Kai
AK
Chen
Yu-Ju
YJ
Lin
Chun-Cheng
CC
D005439:Flufenamic Acid / Q000032:analysis; D006801:Humans; D007660:Ketoprofen / Q000032:analysis; D018942:Macrolides / Q000032:analysis; D008280:Magnetics; D008358:Mannose / Q000032:analysis; D008528:Mefenamic Acid / Q000032:analysis; D015394:Molecular Structure; D053758:Nanoparticles / Q000032:analysis*; D010316:Particle Size; D011239:Prednisolone / Q000032:analysis; D012371:Robotics; D012457:Salicylamides / Q000032:analysis; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000191:economics / Q000295:instrumentation / Q000379:methods; D013467:Sulindac / Q000032:analysis
D018942:Macrolides; D012457:Salicylamides; C076477:concanamycin F; D013467:Sulindac; D008528:Mefenamic Acid; D005439:Flufenamic Acid; D007660:Ketoprofen; D011239:Prednisolone; C031060:salicylamide; D008358:Mannose