title: Breast cancer prevention by green tea catechins and black tea theaflavins in the C3(1) SV40 T,t antigen transgenic mouse model is accompanied by increased apoptosis and a decrease in oxidative DNA adducts.
abstract: Tea consumption is associated with a reduced risk of mammary cancer as reflected by epidemiological studies and experiments in carcinogen-induced rodent models of mammary carcinogenesis. We tested the hypothesis that green tea catechins (GTC) or theaflavins from black tea (BTT) interfere with mammary carcinogenesis in C3(1) SV40 T,t antigen transgenic multiple mammary adenocarcinoma (TAg) mice and that GTC/BTT affect tumor survival or oxidation status. TAg mice received GTC/BTT (0.05%) in drinking water for their lifetime. As compared to control mice, they survived longer and had smaller tumors. On microscopic inspection, the size of the largest tumor per mouse was decreased by 40-42% (p<0.01). GTC (0.01%) and BTT (0.05%) increased levels of cleaved caspase 3 in tumor tissue by 67 and 38%, respectively (p<0.05), intimating increased apoptosis. Tumor levels of the malondialdehyde-DNA adduct M1dG in mice receiving GTC or BTT (0.05%) were reduced by 78 (p<0.001) or 63% (p<0.05), respectively, as compared to controls. The results render the exploration of the breast cancer chemopreventive properties of tea preparations in humans worthwhile.
authors.affiliation: Department of Cancer Studies and Molecular Medicine, University of Leicester, Leicester LE2 7LX, United Kingdom.
authors.forename: Siminder
authors.initials: S
authors.lastname: Kaur
authors.forename: Peter
authors.initials: P
authors.lastname: Greaves
authors.forename: Darren N
authors.initials: DN
authors.lastname: Cooke
authors.forename: Richard
authors.initials: R
authors.lastname: Edwards
authors.forename: William P
authors.initials: WP
authors.lastname: Steward
authors.forename: Andreas J
authors.initials: AJ
authors.lastname: Gescher
authors.forename: Timothy H
authors.initials: TH
authors.lastname: Marczylo
mesh_terms: D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D017209:Apoptosis / Q000187:drug effects*; D044946:Biflavonoids / Q000008:administration & dosage*; D053148:Caspase 3 / Q000032:analysis; D002392:Catechin / Q000008:administration & dosage*; D045744:Cell Line, Tumor; D018736:DNA Adducts / Q000032:analysis*; D006801:Humans; D015674:Mammary Neoplasms, Animal / Q000517:prevention & control*; D051379:Mice; D008822:Mice, Transgenic; D013662:Tea / Q000737:chemistry*
chemical_list: D000952:Antigens, Polyomavirus Transforming; D044946:Biflavonoids; D018736:DNA Adducts; D013662:Tea; C056068:theaflavin; D002392:Catechin; D053148:Caspase 3