title: Japanese black cattle with orotic aciduria detected by gas-chromatography/mass-spectrometry.
abstract: A 4-months-old calf of Japanese black cattle was diagnosed with orotic aciduria by gas-chromatography/mass-spectrometry (GC/MS). Until now orotic aciduria had not been reported in Japanese black cattle. The animal showed repeated diarrhea. The hematocrit was low, and microcytes and acanthocytes were observed in blood smears. The calf had lower serum total protein concentrations with a higher blood ammonia concentration. Needle-shaped crystals of orotic acid were observed in urinary sediments. Sequence homologous analysis with cattle uridine monophosphate synthase DNA indicated silent mutation in the affected calf.
authors.affiliation: Laboratory of Internal Medicine, Division of Veterinary Medicine, Faculty of Applied Sciences, Gifu University, Japan.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Ohba
authors.forename: Masaki
authors.initials: M
authors.lastname: Takasu
authors.forename: Naohito
authors.initials: N
authors.lastname: Nishii
authors.forename: Iwai
authors.initials: I
authors.lastname: Hosoda
authors.forename: Katsuya
authors.initials: K
authors.lastname: Kitoh
authors.forename: Isamu
authors.initials: I
authors.lastname: Matsumoto
authors.forename: Chunhua
authors.initials: C
authors.lastname: Zhang
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Kitagawa
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000652:urine*; D004252:DNA Mutational Analysis / Q000662:veterinary; D003677:Deficiency Diseases / Q000652:urine / Q000662:veterinary; D017809:Fatal Outcome; D008401:Gas Chromatography-Mass Spectrometry / Q000662:veterinary; D008297:Male; D009097:Multienzyme Complexes / Q000172:deficiency* / Q000235:genetics; D009962:Orotate Phosphoribosyltransferase / Q000172:deficiency* / Q000235:genetics; D009963:Orotic Acid / Q000097:blood / Q000652:urine*; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000172:deficiency* / Q000235:genetics; D010375:Pedigree
chemical_list: D009097:Multienzyme Complexes; D009963:Orotic Acid; C027157:uridine 5'-monophosphate synthase; D009962:Orotate Phosphoribosyltransferase; D009964:Orotidine-5'-Phosphate Decarboxylase