[Observation of an injectable tissue-engineered bone constructed with autologous platelet-rich plasma and bone marrow stromal cells].
OBJECTIVE
To construct an injectable tissue-engineered bone graft with fibrin glue (FG), autologous platelet-rich plasma (PRP) and bone marrow stromal cells (BMSCs) cultured in vitro and study its biological characteristics and microscopic structures.


METHODS
BMSCs isolated from rabbit iliac bone marrow were culture-expanded in vitro. The injectable tissue-engineered bone constructed from autologous PRP, FG, and BMSCs was cultured in vitro, and its biological characteristics were observed including the time of gel formation, histological features, seed cell survival and microscopic structures.


RESULTS
The constructed injectable tissue-engineered bone began gel formation within 20 to 30 s, and after a week-long culture, the gelatine began to degrade, and numerous well viable fusiform cells could be seen to adhere to the bottom of the Petri dish. Scanning electron microscopy identified globular and olivary cells embedded in the fibrin glue, and numerous small particles could be seen around of the cells.


CONCLUSIONS
Construction of an injectable tissue-engineered bone graft with FG, BMSCs and PRP does not require sophisticated techniques and ensures good biological property of the bone graft that can be easily shaped and allow good growth of the seed cells, suggesting great potential of this technique for clinical use.
Huang
Ai-wen
AW
Department of Orthopedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. huangtuyuan@163.com
Jin
Dan
D
Peng
Dao-bo
DB
Pei
Guo-xian
GX
D001854:Bone Marrow Cells / Q000166:cytology*; D018786:Bone Substitutes / Q000737:chemistry*; D002478:Cells, Cultured; D018920:Coculture Techniques; D015718:Fibrin Tissue Adhesive / Q000737:chemistry; D053657:Platelet-Rich Plasma / Q000737:chemistry*; D017154:Stromal Cells / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods*
D018786:Bone Substitutes; D015718:Fibrin Tissue Adhesive