title: Characterization of tccP2 carried by atypical enteropathogenic Escherichia coli.
abstract: Atypical enteropathogenic Escherichia coli (EPEC) comprise an important group of paediatric pathogens. Atypical EPEC have reservoirs in farm and domestic animals where they can be either commensal or pathogenic; serogroup O26 is dominant in humans and animals. Central to intestinal colonization by EPEC is the translocation of the type III secretion system effector Tir into enterocytes, which following phosphorylation (Tir-Yp) recruits Nck to activate the N-WASP actin signalling cascade. The authors have recently shown that typical EPEC strains, belonging to the EPEC-2 lineage, carry a tir gene encoding Tir-Yp and can also use the alternative TccP2 actin-signalling cascade. The aim of this study was to determine if tccP2 is found in atypical EPEC isolated from human and farm animals. tccP2 was found at a frequency of 41% in non-O26 EPEC isolates and in 82.3% of the O26 strains. TccP2 of human and animal strains show high level of sequence identity. It is shown that most strains carry a tir gene encoding Tir-Yp. In addition the authors identified two new variants of tir genes in EPEC O104:H12 and NT:H19 strains.
authors.affiliation: Division of Bioenvironmental Science, Frontier Science Research Center, University of Miyazaki, Miyazaki, Japan.
authors.forename: Tadasuke
authors.initials: T
authors.lastname: Ooka
authors.forename: Mônica A M
authors.initials: MA
authors.lastname: Vieira
authors.forename: Yoshitoshi
authors.initials: Y
authors.lastname: Ogura
authors.forename: Lothar
authors.initials: L
authors.lastname: Beutin
authors.forename: Roberto
authors.initials: R
authors.lastname: La Ragione
authors.forename: Pauline M
authors.initials: PM
authors.lastname: van Diemen
authors.forename: Mark P
authors.initials: MP
authors.lastname: Stevens
authors.forename: Ilknur
authors.initials: I
authors.lastname: Aktan
authors.forename: Shaun
authors.initials: S
authors.lastname: Cawthraw
authors.forename: Angus
authors.initials: A
authors.lastname: Best
authors.forename: Rodrigo T
authors.initials: RT
authors.lastname: Hernandes
authors.forename: Gladys
authors.initials: G
authors.lastname: Krause
authors.forename: Tania A T
authors.initials: TA
authors.lastname: Gomes
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Hayashi
authors.forename: Gad
authors.initials: G
authors.lastname: Frankel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000829:Animals, Domestic; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D004927:Escherichia coli Infections / Q000382:microbiology* / Q000662:veterinary*; D029968:Escherichia coli Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C505370:tccP protein, E coli