Nanostructuring of heme-proteins for biodevice applications.
Proteins represent versatile building blocks for the realisation of nanostructured materials to be applied in the nanobiotechnological field. The Langmuir-Blodgett (LB) technique was utilised as a means to develop nanobiodevices based on protein molecules. Namely, engineered Cytochrome P450 thin films were fabricated and characterised. The possibility to employ LB-based protein structures to use in biosensors has been exploited. The characterisation process was performed in order to verify the best working parameters. As a first step' the protein films were studied at the air-water interface and then transferred into a solid support for further characterisation. The films were characterised by different techniques such as: UV-vis spectroscopy, nanogravimetry, atomic force microscopy and biochemical assays. The results showed that it was possible to form active cytochrome P450s nanostructures by the LB technique.
Paternolli
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C
Fondazione Elba, Roma, ItalyUniversity of Genova, Nanoworld Institute and Biophysics Division, Genova, Italy. manuscript@ibf.unige.it
Ghisellini
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P
Nicolini
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C
D000327:Adsorption; D001681:Biological Assay / Q000295:instrumentation / Q000379:methods*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003460:Crystallization / Q000379:methods; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry* / Q000648:ultrastructure*; D004789:Enzyme Activation; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D011485:Protein Binding; D015202:Protein Engineering / Q000379:methods*
D020099:Coated Materials, Biocompatible; D003577:Cytochrome P-450 Enzyme System