title: Comprehensive separation of secondary metabolites in natural products by high-speed counter-current chromatography using a three-phase solvent system.
abstract: High-speed counter-current chromatography (HSCCC) using the three-phase solvent system n-hexane-methyl acetate-acetonitrile-water at a volume ratio of 4:4:3:4 was applied to the comprehensive separation of secondary metabolites in several natural product extracts. A wide variety of secondary metabolites in each natural product was effectively extracted with the three-phase solvent system, and the filtered extract was directly submitted to the HSCCC separation using the same three-phase system. In the HSCCC profiles of crude natural drugs listed in the Japanese Pharmacopoeia, several physiologically active compounds were clearly separated from other components in the extracts. The HSCCC profiles of several tea products, each manufactured by a different process, clearly showed their compositional difference in main compounds such as catechins, caffeine, and pigments. These HSCCC profiles also provide useful information about hydrophobic diversity of whole components present in each natural product.
authors.affiliation: Division of Structural Biology and Analytical Science, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan. yanagida@ps.toyaku.ac.jp
authors.forename: Akio
authors.initials: A
authors.lastname: Yanagida
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yamakawa
authors.forename: Ryoko
authors.initials: R
authors.lastname: Noji
authors.forename: Ako
authors.initials: A
authors.lastname: Oda
authors.forename: Heisaburo
authors.initials: H
authors.lastname: Shindo
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Ito
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Shibusawa
mesh_terms: D002110:Caffeine / Q000032:analysis; D002212:Capsicum / Q000737:chemistry; D002392:Catechin / Q000032:analysis; D003377:Countercurrent Distribution / Q000379:methods*; D020939:Zingiber officinale / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry*; D013662:Tea / Q000737:chemistry
chemical_list: D010936:Plant Extracts; D012997:Solvents; D013662:Tea; D002110:Caffeine; D002392:Catechin