title: [Effects of (60)Co gamma rays on the biological behaviour of lung fibroblasts in C57BL/6J and C3H/HeN mice].
abstract: OBJECTIVE
  To investigate the effects of (60)Co gamma rays on the biological behaviour of lung fibroblasts (LFs) in C57BL/6J and C3H/HeN mice.
  
  
  METHODS
  LFs in C57BL/6J and C3H/HeN mice were isolated, cultured and irradiated by 2, 4, 6 and 8 Gy (60)Co gamma rays. The proliferative activity and cell cycle were detected by MTT colorimetry, AgNOR staining and fluorescence-activated cell sorting(FACS). Then the expressions of alpha-smooth muscle actin (a-SMA), matrix metal proteinase (MMP-1) and tissue inhibitor of metalloproteinase (TIMP-1) in LFs were observed by immunocytochemical staining.
  
  
  RESULTS
  The proliferation of LFs in C57BL/6J mice was not promoted by 2, 4, 6 and 8 Gy (60)Co gamma rays, but the number of aneuploid cells increased. MMP-1 was weak positive. The expression of a-SMA and TIMP-1 was enhanced by (60)Co gamma rays. The proliferation of LFs in C3H/HeN mice was not promoted by 2, 4, 6 and 8 Gy (60)Co gamma rays either, but the number of cells in G2-M phase increased. The expression of MMP-1 and TIMP-1 was enhanced, but the expression of a-SMA was decreased by (60)Co gamma rays.
  
  
  CONCLUSIONS
  The biological behaviours of the irradiated LFs in C57BL/6J and C3H/HeN mice are different. LFs in C57BL/6J mice can be pactivatedq. Our study will provide a celluar basis for the researches into the C57BL/6J mice which are prone to radiation pulmonary fibrosis (RPF).
authors.affiliation: Beijing Institute of Radiation Medicine, Beijing 100850, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Rui-Yun
authors.initials: RY
authors.lastname: Peng
authors.forename: Shui-Ming
authors.initials: SM
authors.lastname: Wang
authors.forename: Ya-Bing
authors.initials: YB
authors.lastname: Gao
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Ma
authors.forename: Liang-Wen
authors.initials: LW
authors.lastname: Song
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002453:Cell Cycle / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects*; D005434:Flow Cytometry; D005720:Gamma Rays; D015870:Gene Expression / Q000528:radiation effects; D007150:Immunohistochemistry; D008168:Lung / Q000166:cytology*; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism
chemical_list: D000199:Actins; D019715:Tissue Inhibitor of Metalloproteinase-1; D020781:Matrix Metalloproteinase 1