title: [Studies for analyzing restricted ingredients such as phenylbenzoimidazole sulfonic acid].
abstract: Phenylbenzoimidazol sulfonic acid (PBS) is a kind of sunscreens in cosmetics and is nominated as the restricted ingredients in cosmetics in Japanese Pharmaceutical Affairs Act. So the analytical method for PBS was investigated by HPLC. 1.0 g of the lotions with 1.0% PBS was exactly weighed, put into a 50-mL volumetric flask. Water was added to make exactly 50 mL and this mixture was used as the sample solution. On the other hand, 1.0 g of the creams with 1.0% PBS was exactly weighed, put into a beaker. After adding 1 mL of tetrahydrofuran and dissolving the cream, that mixture was transferred to a 50-mL volumetric flask. And then the beaker was rinsed with 1 mL of tetrahydrofuran and the rinsed solution was put together into the volumetric flask. After adding water to the volumetric flask to make exactly 50 mL, this mixture was used as the sample solution. If necessary, the mixture was filtrated with a membrane filter (0.45 microm). 5.0 mL of the sample solution was pipetted and put into a 200-mL volumetric flask. After adding water to make exactly 200 mL, 20 microL of this solution was analyzed by HPLC using the ODS column (CAPCELL PAK C18 column, 4.6 mm i.d. x 250 mm), the mixture of 40 mmol/L acetic buffer (pH 3.4) and acetonitrile (3:1) with 0.8 mmol/L dodecyltrimethyl ammonium bromide and the detection wavelength of 305 nm. The working curve from 0.5 to 20.0 microg/mL showed a linear line between the concentrations of PBS and the peak areas. There was no interference of peak of PBS from the lotion and cream.
authors.affiliation: tokunaga@nihs.go.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tokunaga
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Mori
authors.forename: Nahomi
authors.initials: N
authors.lastname: Onuki
authors.forename: Tomio
authors.initials: T
authors.lastname: Nosaka
authors.forename: Kayo
authors.initials: K
authors.lastname: Doi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sakaguchi
authors.forename: Makiko
authors.initials: M
authors.lastname: Fujii
authors.forename: Katuhiro
authors.initials: K
authors.lastname: Takano
authors.forename: Masato
authors.initials: M
authors.lastname: Hayashi
authors.forename: Kenichi
authors.initials: K
authors.lastname: Yoshizawa
authors.forename: Kimio
authors.initials: K
authors.lastname: Shimamura
authors.forename: Nobuo
authors.initials: N
authors.lastname: Sato
mesh_terms: D001562:Benzimidazoles / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D007564:Japan; D007880:Legislation, Drug; D013451:Sulfonic Acids / Q000032:analysis*; D013473:Sunscreening Agents / Q000737:chemistry*
chemical_list: D001562:Benzimidazoles; D013451:Sulfonic Acids; D013473:Sunscreening Agents; C499767:ensulizole