Neuroprotective effect of ganglioside GM1 on the cytotoxic action of hydrogen peroxide and amyloid beta-peptide in PC12 cells.
Ganglioside GM1 was shown to increase the viability of PC12 cells exposed to hydrogen peroxide or amyloid beta-peptide (Abeta(25-35)). The PC12 cells transfected with mutant gene (expressing APP(SW)) were found to be more sensitive to oxidative stress than the cells transfected with wild type gene (expressing APP(WT)) or vector-transfected cells, GM1 being effective in enhancing the viability of the cells transfected with mutant gene. The exposure to hydrogen peroxide or Abeta(25-35) results in a partial inactivation of Na(+),K(+)-ATPase in PC12 cells, H(2)O(2) increases MDA accumulation in these cells. But these effects could be partially prevented or practically abolished by GM1 ganglioside. In the presence of the inhibitor of tyrosine kinase of Trk receptors (K-252a) the protective and metabolic effects of GM1 on PC12 cells in conditions of oxidative stress caused by hydrogen peroxide are not observed or are markedly diminished.
Sokolova
Tatyana V
TV
Department of Comparative Neurochemistry, Institute of Evolutionary Physiology and Biochemistry of Russian Academy of Sciences, St. Petersburg, Russia.
Zakharova
Irina O
IO
Furaev
Victor V
VV
Rychkova
Maria P
MP
Avrova
Natalia F
NF
D016229:Amyloid beta-Peptides / Q000235:genetics / Q000378:metabolism / Q000633:toxicity*; D000818:Animals; D002227:Carbazoles / Q000378:metabolism; D002417:Cattle; D002470:Cell Survival; D004791:Enzyme Inhibitors / Q000378:metabolism; D005677:G(M1) Ganglioside / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D026121:Indole Alkaloids / Q000378:metabolism; D018696:Neuroprotective Agents / Q000378:metabolism; D016877:Oxidants / Q000633:toxicity*; D018384:Oxidative Stress; D016716:PC12 Cells; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism / Q000633:toxicity*; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000378:metabolism
D016229:Amyloid beta-Peptides; D002227:Carbazoles; D004791:Enzyme Inhibitors; D026121:Indole Alkaloids; D018696:Neuroprotective Agents; D016877:Oxidants; D010446:Peptide Fragments; C074636:amyloid beta-protein (25-35); D005677:G(M1) Ganglioside; C049985:staurosporine aglycone; D006861:Hydrogen Peroxide; D000254:Sodium-Potassium-Exchanging ATPase