title: A major zebrafish polymorphism resource for genetic mapping.
abstract: We have identified 645,088 candidate polymorphisms in zebrafish and observe a single nucleotide polymorphism (SNP) validation rate of 71% to 86%, improving with polymorphism confidence score. Variant sites are non-random, with an excess of specific novel T- and A-rich motifs. We positioned half of the polymorphisms on zebrafish genetic and physical maps as a resource for positional cloning. We further demonstrate bulked segregant analysis using the anchored SNPs as a method for high-throughput genetic mapping in zebrafish.
authors.affiliation: Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232-0275, USA. Kevin.bradley@vanderbilt.edu
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Bradley
authors.forename: J Bradford
authors.initials: JB
authors.lastname: Elmore
authors.forename: Joan P
authors.initials: JP
authors.lastname: Breyer
authors.forename: Brian L
authors.initials: BL
authors.lastname: Yaspan
authors.forename: Jason R
authors.initials: JR
authors.lastname: Jessen
authors.forename: Ela W
authors.initials: EW
authors.lastname: Knapik
authors.forename: Jeffrey R
authors.initials: JR
authors.lastname: Smith
mesh_terms: D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D003001:Cloning, Molecular; D008040:Genetic Linkage; D005819:Genetic Markers; D020641:Polymorphism, Single Nucleotide; D015027:Zebrafish / Q000235:genetics*
chemical_list: D005819:Genetic Markers