The apyrase KlYnd1p of Kluyveromyces lactis affects glycosylation, secretion, and cell wall properties.
The Kluyveromyces lactis ORF r_klactIV3,463 on chromosome IV, hereafter named KlYND1, encodes an endoapyrase that has nucleoside phosphatase activity with a lumenal orientation. The enzyme showed equally high activity towards GDP/UDP and ADP, and also showed activity, although to a lesser extent, towards GTP. No activity was detected with the other triphosphates and all monophosphates. The overexpression of KlYND1 in Klgda1Delta cells of K. lactis, devoid of the encoded GDPase/UDPase activity, suppressed the loss of O-glycosylation and cell wall-related defects described in such mutants, and suggests a partial overlap of function between the two genes, and therefore some redundancy. The overexpression of KlYND1 in wild-type cells enhanced the secretion of the recombinant human serum albumin and glucoamylase employed as reporters.
Uccelletti
Daniela
D
Department of Developmental and Cell Biology, University of Rome La Sapienza, Rome, Italy.
Anticoli
Simona
S
Palleschi
Claudio
C
D001081:Apyrase / Q000235:genetics* / Q000378:metabolism* / Q000494:pharmacology; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D005786:Gene Expression Regulation; D005087:Glucan 1,4-alpha-Glucosidase / Q000235:genetics / Q000378:metabolism; D006031:Glycosylation / Q000187:drug effects; D006801:Humans; D007716:Kluyveromyces / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D008969:Molecular Sequence Data; D010641:Phenotype; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D012709:Serum Albumin / Q000235:genetics / Q000378:metabolism
D005656:Fungal Proteins; D011994:Recombinant Proteins; D012709:Serum Albumin; D005087:Glucan 1,4-alpha-Glucosidase; D001081:Apyrase