Application of fractional factorial experimental and Box-Behnken designs for optimization of single-drop microextraction of 2,4,6-trichloroanisole and 2,4,6-tribromoanisole from wine samples.
In this paper a new method for the determination of 2,4,6-trichloroanisole (TCA) and 2,4,6-tribromoanisole (TBA) in wine samples is presented. Headspace single-drop microextraction (HS-SDME) was used for the extraction and preconcentration of the analytes, followed by analysis by gas chromatography and electron-capture detection (GC-ECD). The variables affecting extraction efficiency were optimized using fractional factorial experimental and Box-Behnken designs. The external calibration procedure was successfully carried out using a synthetic wine solution and diluted red wine samples. The method was also applied to white wine samples. Excellent detection limits of 8.1 and 6.1 ng L(-1) were achieved for TCA and TBA, respectively. Good precision and accuracy were obtained.
Martendal
Edmar
E
Departamento de Química, Universidade Federal de Santa Catarina, Florianópolis, SC 88040-900, Brazil.
Budziak
Dilma
D
Carasek
Eduardo
E
D000840:Anisoles / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D002138:Calibration; D005591:Chemical Fractionation / Q000379:methods*; D002849:Chromatography, Gas; D015203:Reproducibility of Results; D014920:Wine / Q000032:analysis*
D000840:Anisoles; C110228:2,4,6-trichloroanisole; C484217:2,4,6-tribromoanisole