[Similar nucleotide blocks in tobamoviral subgenomic promoters].
It was discovered by computational analysis of nucleotide sequences that the movement (MP) and coat proteins (CP) subgenomic RNA promoter regions of the most tobamoviruses tested (10 from 15) contain almost identical three-component nucleotide blocks. In the middle part of the block there is the central nine-nucleotide motive GGTTCGTTT or GATTCGTTT, flanged with length-variable GC- and AT-containing sequences. GC-containing sites are localized upstream of the central motive and possess the CGC motive. The sixth nucleotide of central motives (G) is located in genomes 65-81 nucleotides upstream of MP gene or 9-20 nucleotides upstream of CP gene. The others tobamoviruses tested (5 from 15) have a considerable quantity of nucleotide replacements in central motives, high variability of GC-containing sites as well as two similar nucleotide blocks in promoters of CP genes. The role of the similar nucleotide blocks as functional elements of tobamoviral subgenomic RNA promoters is discussed.
Hordieĭchyk
O I
OI
Oleshchenko
L T
LT
Shcherbatenko
I S
IS
D001483:Base Sequence; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009711:Nucleotides / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D017898:Tobamovirus / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
D009711:Nucleotides; D012367:RNA, Viral; D014764:Viral Proteins