Toll-like receptor 4 Asp299Gly polymorphism is associated with an increased risk of pancreatic necrotic infection in acute pancreatitis: a study in the Chinese population.
OBJECTIVE
To detect the frequency of Toll-like receptor (TLR) 4896A>G (Asp299Gly) polymorphism in acute pancreatitis (AP) patients and to determine the relationship between polymorphism and pancreatic necrotic infection.


METHODS
The allele frequency of TLR4 Asp299Gly was surveyed by mispairing polymerase chain reaction-restriction fragment length polymorphism analysis technique in AP patients with pancreatic necrosis and healthy volunteers, respectively.


RESULTS
All individuals with TLR4 Asp299Gly polymorphism were heterozygotes. Compared with healthy volunteers (5.6%), TLR4 896G allele frequencies was significantly higher in AP patients with pancreatic infection (20%), the incidence of gram-negative infection was significantly higher in AP patients with TLR4 Asp299Gly polymorphism (15 [44%]/34) than that in AP patients without TLR4 polymorphism (15 [18%]/81).


CONCLUSIONS
Toll-like receptor 4 Asp299Gly polymorphism is associated with the infection of pancreatic necrosis in AP. Patients who carried TLR4 896A>G mutation is susceptible to pancreatic gram-negative bacteria infection.
Gao
Hong-Kai
HK
Department of General Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan Province, China.
Zhou
Zong-Guang
ZG
Li
Yuan
Y
Chen
You-Qin
YQ
D018806:APACHE; D000328:Adult; D019943:Amino Acid Substitution; D001224:Aspartic Acid; D001424:Bacterial Infections / Q000150:complications; D002681:China; D005260:Female; D005787:Gene Frequency; D014644:Genetic Variation; D005998:Glycine; D006801:Humans; D008297:Male; D008875:Middle Aged; D009336:Necrosis; D010195:Pancreatitis / Q000209:etiology / Q000235:genetics* / Q000382:microbiology / Q000473:pathology*; D011110:Polymorphism, Genetic; D015183:Restriction Mapping; D012307:Risk Factors; D051197:Toll-Like Receptor 4 / Q000235:genetics*
C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4; D001224:Aspartic Acid; D005998:Glycine