Construction of mouse adenovirus type 1 mutants.
Mouse adenovirus provides a model for studying adenovirus pathogenesis in the natural host. The ability to make viral mutants allows the investigation of specific mouse adenoviral gene contributions to virus-host interactions. Methods for propagation and titration of wild-type mouse adenovirus, production of viral DNA and viral DNA-protein complex, and transfection of mouse cells to obtain mouse adenovirus mutants are described in this chapter. Plaque purification, propagation, and titration of the mutant viruses are also presented.
Cauthen
Angela N
AN
Department of Natural Sciences, Clayton College and State University, Morrow, GA, USA.
Welton
Amanda R
AR
Spindler
Katherine R
KR
D000256:Adenoviridae / Q000235:genetics* / Q000302:isolation & purification*; D000818:Animals; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D007202:Indicators and Reagents; D051379:Mice; D009154:Mutation; D014162:Transfection / Q000379:methods; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000235:genetics / Q000302:isolation & purification
D004279:DNA, Viral; D007202:Indicators and Reagents; D014764:Viral Proteins; D004247:DNA