Baicalin protects mouse from Concanavalin A-induced liver injury through inhibition of cytokine production and hepatocyte apoptosis.
BACKGROUND
Baicalin (BA) exhibits an anti-inflammatory effect in vivo and in vitro and is used to treat chronic hepatitis. However, the mechanism by which BA exerts the liver-protective effect remains largely unknown.


OBJECTIVE
The present study reports that BA inhibits cytokine production and hepatocyte apoptosis to protect mice from liver injury induced by concanavalin A (Con A), a T-cell-dependent liver injury model.


RESULTS
Con A injection of mice induced severe immune responses and extensive hepatocellular apoptosis within 24 h. Pretreatment of 200 or 100 mg/kg BA markedly reduced serum aminotransferase activities, protected hepatoycte apoptosis and reduced the increase of plasma cytokine levels, including tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma) and interleukin-6 (IL-6). Furthermore, BA pretreatment decreased tissue myeloperoxidase activity and lipid peroxidation, but increased the superoxide dismutase level. In vitro studies indicated that the beneficial effect of BA was associated with reduced cytokine production from lymphocytes and reduced TNF-alpha-induced hepatocyte apoptosis.


CONCLUSIONS
These results suggest that BA has therapeutic potential for T-cell-mediated liver injury.
Liu
Lin-Lin
LL
State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
Gong
Li-Kun
LK
Wang
Hui
H
Xiao
Ying
Y
Wu
Xiong-Fei
XF
Zhang
Yun-Hai
YH
Xue
Xiang
X
Qi
Xin-Ming
XM
Ren
Jin
J
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy / Q000473:pathology / Q000517:prevention & control*; D003208:Concanavalin A / Q000009:adverse effects*; D016207:Cytokines / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D005419:Flavonoids / Q000494:pharmacology*; D022781:Hepatocytes / Q000187:drug effects*; D051379:Mice; D008815:Mice, Inbred Strains; D020011:Protective Agents / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology; D016896:Treatment Outcome; D014409:Tumor Necrosis Factor-alpha
D016207:Cytokines; D005419:Flavonoids; D020011:Protective Agents; D014409:Tumor Necrosis Factor-alpha; D003208:Concanavalin A; C038044:baicalin