Lack of association between the apolipoprotein E gene and aneurysmal subarachnoid hemorrhage in an Italian population.
OBJECTIVE
The results of genome-wide scan studies have suggested the presence of a genetic risk factor for aneurysmal subarachnoid hemorrhage (SAH) on chromosome 19 (at 19p13). The apolipoprotein E (APOE) gene is located in this chromosomal region and encodes a protein that exerts several neuroprotective and neurotrophic functions in the brain. The purpose of this study was to evaluate whether a particular allele or genotype of the APOE gene would modify the occurrence or the clinical features of SAH.


METHODS
Genomic DNA was extracted from 146 patients with aneurysmal SAH and 222 age- and sex-matched healthy controls and genotyped for the triallelic polymorphism of the APOE gene (epsilon2, epsilon3, and epsilon4). Allele and genotype frequencies were compared between patients and controls. The clinical characteristics of the disease were compared according to the different APOE genotypes. Allele and genotype frequencies of the APOE gene polymorphism were nearly identical in cases and controls. Patients carrying the APOE epsilon4 allele had a significantly higher Hunt and Hess grade on admission (p = 0.0014). There was no significant relationship between any of the other clinical characteristics and the APOE genotype.


CONCLUSIONS
The authors' data do not support the hypothesis that genetic variations within the APOE gene are associated with aneurysmal SAH. However, the APOE gene influences the disease phenotype and may be regarded as a disease modifier gene.
Fontanella
Marco
M
Division of Neurosurgery, Department of Neuroscience, University of Turin, Italy. marco.fontanella@unito.it
Rainero
Innocenzo
I
Gallone
Salvatore
S
Rubino
Elisa
E
Rivoiro
Chiara
C
Valfrè
Walter
W
Garbossa
Diego
D
Nurisso
Chiara
C
Ducati
Alessandro
A
Pinessi
Lorenzo
L
D000328:Adult; D000368:Aged; D001057:Apolipoproteins E / Q000235:genetics*; D016022:Case-Control Studies; D015331:Cohort Studies; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D023261:Glasgow Outcome Scale; D006801:Humans; D007558:Italy; D008297:Male; D008875:Middle Aged; D013345:Subarachnoid Hemorrhage / Q000208:ethnology / Q000235:genetics*
D001057:Apolipoproteins E