title: Wild type and H43Y variant of human TRIM5alpha show similar anti-human immunodeficiency virus type 1 activity both in vivo and in vitro.
abstract: Polymorphisms in human genes have been shown to affect the rate of disease progression to acquired immune deficiency syndrome in human immunodeficiency virus type 1 (HIV-1)-infected individuals. Recently, tripartite motif 5alpha (TRIM5alpha) was identified as a factor that confers resistance to HIV-1 infection in Old World monkey cells. Subsequently, Sawyer et al. (Curr Biol 16:95-100, 2006) reported a single nucleotide polymorphism (H43Y) in the human TRIM5alpha gene and TRIM5alpha protein with 43Y was found to lose its ability to restrict HIV-1. In the present study, we reevaluated effects of this allele on in vitro anti-HIV-1 activity as well as on HIV-1 disease progression in European and Asian cohorts of HIV-1-infected individuals. Our epidemiological and molecular biological findings clearly indicate H43Y has a very minor effect on anti-HIV-1 activity of TRIM5alpha, suggesting that this allele is immaterial, at least in HIV-1-infected Europeans and Asians.
authors.affiliation: Department of Viral Infections, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamada-oka, Suita-shi, Osaka, Japan.
authors.forename: Emi E
authors.initials: EE
authors.lastname: Nakayama
authors.forename: Wassila
authors.initials: W
authors.lastname: Carpentier
authors.forename: Dominique
authors.initials: D
authors.lastname: Costagliola
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Shioda
authors.forename: Aikichi
authors.initials: A
authors.lastname: Iwamoto
authors.forename: Patrice
authors.initials: P
authors.lastname: Debre
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Yoshimura
authors.forename: Brigitte
authors.initials: B
authors.lastname: Autran
authors.forename: Shuzo
authors.initials: S
authors.lastname: Matsushita
authors.forename: Ioannis
authors.initials: I
authors.lastname: Theodorou
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D000090103:Antiviral Restriction Factors; D044466:Asian People; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics*; D002460:Cell Line; D006224:Cricetinae; D020022:Genetic Predisposition to Disease; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D009154:Mutation; D029082:Sendai virus / Q000235:genetics; D000070584:Tripartite Motif Proteins; D044767:Ubiquitin-Protein Ligases; D014779:Virus Replication; D044465:White People
chemical_list: D000090103:Antiviral Restriction Factors; D002352:Carrier Proteins; D000070584:Tripartite Motif Proteins; C477346:TRIM5 protein, human; D044767:Ubiquitin-Protein Ligases