title: Evaluation of porcine myocardial microvascular permeability and fractional vascular volume using 64-slice helical computed tomography (CT).
abstract: OBJECTIVE
  Myocardial microvascular permeability-surface area product (MPSP) and fractional vascular volume (FVV), indices of endothelial function and microvascular perfusion, can be noninvasively evaluated by electron beam computed tomography (EBCT), but it remains unknown whether comparable assessments can be obtained with 64-slice multidetector CT (CT-64).
  
  
  METHODS
  We studied 12 pigs with both EBCT and CT-64 in randomized order 1 week apart, before and during IV adenosine infusion. Myocardial attenuation changes in the cardiac wall were assessed after a central-venous injection of iopamidol. Time-attenuation curves were analyzed using both indicator-dilution and Patlak models to calculate MPSP and FVV.
  
  
  RESULTS
  CT-64 and EBCT assessments of basal MPSP obtained by the Patlak method were similar (0.37 +/- 0.03 vs. 0.37 +/- 0.04 mL/min/g), as was its response to adenosine, and correlated significantly (r = 0.87). Patlak FVV was also similar between CT-64 and EBCT at baseline (0.08 +/- 0.02 vs. 0.07 +/- 0.02 mL blood/mL) and during adenosine, and correlated well (r = 0.93). MPSP and FVV estimated by the indicator-dilution method were not significantly correlated.
  
  
  CONCLUSIONS
  CT-64 assessments of myocardial MPSP and FVV may not be reliable when using indicator-dilution analysis, likely due to its sensitivity to scan duration. However, CT-64 assessments obtained using the Patlak model are feasible.
authors.affiliation: Department of Medicine, Division of Nephrology and Hypertension, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA.
authors.forename: Elena
authors.initials: E
authors.lastname: Daghini
authors.forename: Andrew N
authors.initials: AN
authors.lastname: Primak
authors.forename: Alejandro R
authors.initials: AR
authors.lastname: Chade
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Zhu
authors.forename: Erik L
authors.initials: EL
authors.lastname: Ritman
authors.forename: Cynthia H
authors.initials: CH
authors.lastname: McCollough
authors.forename: Lilach O
authors.initials: LO
authors.lastname: Lerman
mesh_terms: D000241:Adenosine; D000818:Animals; D001810:Blood Volume / Q000502:physiology*; D002199:Capillary Permeability / Q000502:physiology*; D003287:Contrast Media / Q000008:administration & dosage; D017023:Coronary Angiography; D006321:Heart / Q000000981:diagnostic imaging*; D007479:Iopamidol / Q000008:administration & dosage; D008955:Models, Cardiovascular; D009206:Myocardium; D013552:Swine; D036542:Tomography, Spiral Computed; D014057:Tomography, X-Ray Computed; D014665:Vasodilator Agents
chemical_list: D003287:Contrast Media; D014665:Vasodilator Agents; D007479:Iopamidol; D000241:Adenosine