title: Cytotoxic activity of a dichloromethane extract and fractions obtained from Eudistoma vannamei (Tunicata: Ascidiacea).
abstract: This study consists of the bioassay-guided fractionation of the dichloromethane extract from Eudistoma vannamei and the pharmacological characterization of the active fractions. The dried hydromethanolic extract dissolved in aqueous methanol was partitioned with dichloromethane and chromatographed on a silica gel flash column. The anti-proliferative effect was monitored by the MTT assay. Four of the latest fractions, numbered 14 to 17, which held many chemical similarities amongst each other, were found to be the most active. The selected fractions were tested for viability, proliferation and death induction on cultures of HL-60 promyeloblastic leukemia cells. The results suggested that the observed cytotoxicity is related to apoptosis induction.
authors.affiliation: Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, P.O. Box-3157, 60430-270, Fortaleza, Ceará, Brazil.
authors.forename: Paula C
authors.initials: PC
authors.lastname: Jimenez
authors.affiliation: Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, P.O. Box-3157, 60430-270, Fortaleza, Ceará, Brazil.
authors.forename: Diego V
authors.initials: DV
authors.lastname: Wilke
authors.affiliation: Departamento de Física e Química, Faculdade de Ciências, Filosofia e Letras de Ribeirão Preto, Universidade de São Paulo, Av. do Café s/n, 14040-903, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Renata
authors.initials: R
authors.lastname: Takeara
authors.affiliation: Instituto de Ciências do Mar, Universidade Federal do Ceará, Av. Abolição 3207, 60165-082, Fortaleza, Ceará, Brazil.
authors.forename: Tito M C
authors.initials: TMC
authors.lastname: Lotufo
authors.affiliation: Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, P.O. Box-3157, 60430-270, Fortaleza, Ceará, Brazil.
authors.forename: Cláudia
authors.initials: C
authors.lastname: Pessoa
authors.affiliation: Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, P.O. Box-3157, 60430-270, Fortaleza, Ceará, Brazil.
authors.forename: Manoel
authors.initials: M
authors.lastname: Odorico de Moraes
authors.affiliation: Departamento de Física e Química, Faculdade de Ciências, Filosofia e Letras de Ribeirão Preto, Universidade de São Paulo, Av. do Café s/n, 14040-903, Ribeirão Preto, São Paulo, Brazil. Electronic address: npelopes@fcfrp.usp.br.
authors.forename: Norberto P
authors.initials: NP
authors.lastname: Lopes
authors.affiliation: Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, P.O. Box-3157, 60430-270, Fortaleza, Ceará, Brazil. Electronic address: lvcosta@secrel.com.br.
authors.forename: Letícia V
authors.initials: LV
authors.lastname: Costa-Lotufo
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002855:Chromatography, Thin Layer; D054697:Cytostatic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D003603:Cytotoxins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis; D018922:HL-60 Cells; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000188:drug therapy* / Q000473:pathology; D008752:Methylene Chloride; D019906:Nuclear Magnetic Resonance, Biomolecular; D014561:Urochordata / Q000737:chemistry*
chemical_list: D054697:Cytostatic Agents; D003603:Cytotoxins; D008752:Methylene Chloride; D004247:DNA