Altered expression of plant lysyl tRNA synthetase promotes tRNA misacylation and translational recoding of lysine.
The Arabidopsis thaliana lysyl tRNA synthetase (AtKRS) structurally and functionally resembles the well-characterized prokaryotic class IIb KRS, including the propensity to aminoacylate tRNA(Lys) with suboptimal identity elements, as well as non-cognate tRNAs. Transient expression of AtKRS in carrot cells promotes aminoacylation of such tRNAs in vivo and translational recoding of lysine at nonsense codons. Stable expression of AtKRS in Zea mays causes translational recoding of lysine into zeins, significantly enriching the lysine content of grain.
Wu
Xing Rong
XR
Department of Biochemistry, 117 Schweitzer Hall, University of Missouri-Columbia, Columbia, MO 65211, USA.
Kenzior
Alexander
A
Willmot
David
D
Scanlon
Sarah
S
Chen
Zhihong
Z
Topin
Andrey
A
He
Shao Hua
SH
Acevedo
Ashley
A
Folk
William R
WR
D000215:Acylation; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology*; D001483:Base Sequence; D015139:Blotting, Southern; D017931:DNA Primers; D008239:Lysine / Q000235:genetics*; D008250:Lysine-tRNA Ligase / Q000235:genetics*; D008969:Molecular Sequence Data; D012357:RNA, Transfer, Lys / Q000235:genetics / Q000378:metabolism*; D003313:Zea mays / Q000201:enzymology
D017931:DNA Primers; D012357:RNA, Transfer, Lys; D008250:Lysine-tRNA Ligase; D008239:Lysine