Microtiter plate transformation using the LiAc/SS carrier DNA/PEG method.
Here, we describe a protocol that has been adapted for the transformation of yeast cells in 96-well microtiter plates. This protocol can be tailored for multiple applications and is suitable for high-throughput applications. It can be completed in 2-3 h, once the yeast cells have been grown depending on the heat shock used.
Gietz
R Daniel
RD
Department of Biochemistry and Medical Genetics, University of Manitoba, T250-770 Bannatyne Ave., Winnipeg, Manitoba R3E 0W3, Canada.
Schiestl
Robert H
RH
D000085:Acetates; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D004277:DNA, Single-Stranded / Q000235:genetics; D006358:Hot Temperature; D011092:Polyethylene Glycols; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D014170:Transformation, Genetic
D000085:Acetates; D004277:DNA, Single-Stranded; D011092:Polyethylene Glycols; C488804:lithium acetate