Proteomic strategies for the characterization and the early detection of lung cancer.
Biochemical techniques focused on changes in the proteome in benign and malignant conditions have been applied to the study of lung cancer. Although relatively little information is currently available about the human lung proteome, the major goals of the analysis of lung cancer are to better understand tumor biology, to define early detection biomarkers and predictors of tumor behavior, and to identify potential new therapeutic targets. In this review, we summarized the last 10 years of research in this area with emphasis on its application to the early detection of lung cancer. Basic analytical tools, such as two-dimensional gel electrophoresis, mass spectrometry, and protein arrays, are described and placed in the context of lung cancer research.
Massion
Pierre P
PP
Division of Allergy, Pulmonary and Critical Care Medicine, Vanderbilt Ingram Cancer Center, Veterans Administration Medical Center, Nashville, Tennessee 37232-6838, USA. pierre.massion@vanderbilt.edu
Caprioli
Richard M
RM
D014408:Biomarkers, Tumor / Q000097:blood*; D018450:Disease Progression; D042241:Early Diagnosis; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000175:diagnosis*; D008297:Male; D013058:Mass Spectrometry; D009363:Neoplasm Proteins / Q000032:analysis; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D011379:Prognosis; D040081:Protein Array Analysis; D040901:Proteomics / Q000379:methods*; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins