title: Spectrophotometric methods for the simultaneous analysis of meclezine hydrochloride and pyridoxine hydrochloride in bulk drug and pharmaceutical formulations.
abstract: Three new spectrophotometric procedures for the simultaneous determination of pyridoxine hydrochloride and meclezine hydrochloride are described. The first method depends on the application of simultaneous equation to resolve the interference due to spectral overlapping. The analytical signals were measured at 231 and 220 nm. Calibration graphs were established for 1 to 20 microGmL(-1) for pyridoxine hydrochloride and 0.5 to 10 microGmL(-1) for meclezine hydrochloride in binary mixture. In the second method, the determination of pyridoxine hydrochloride and meclezine hydrochloride was performed by measuring the absorbances at 290 and 235 nm in the simple absorbance spectra of their mixture. In third method a yellowish orange complex of pyridoxine hydrochloride was formed with ferric chloride, which absorbs in the visible region with lambda(max) at 445 nm. Calibration curve of complex formation range was conducted in between 20 to 250 microGmL(-1). These methods were validated with respect to accuracy, precision, linearity, limit of detection and quantification. Regression analysis of Beer's plot showed good correlation in a general concentration range of 1 to 20 microGml(-1) with correlation coefficient (r = 0.9999 and 0.9999; CV < 0.858) for pyridoxine hydrochloride, whereas meclezine hydrochloride concentration range 0.5 to 10 microGmL(-1) with correlation coefficient (r = 0.9998 and 0.9998; CV < 0.826). These methods can be readily applied, without any interference from the excipients. The suggested procedures were successfully applied to the determination of these compounds in synthetic mixtures and in pharmaceutical preparations, with high percentage of recovery, good accuracy and precision.
authors.affiliation: Department of Chemistry, University of Karachi, Karachi-75270, Pakistan. msarayne@gmail.com
authors.forename: M Saeed
authors.initials: MS
authors.lastname: Arayne
authors.forename: Najma
authors.initials: N
authors.lastname: Sultana
authors.forename: Farhan Ahmed
authors.initials: FA
authors.lastname: Siddiqui
authors.forename: M Hashim
authors.initials: MH
authors.lastname: Zuberi
authors.forename: Agha Zeeshan
authors.initials: AZ
authors.lastname: Mirza
mesh_terms: D002138:Calibration; D002712:Chlorides; D005290:Ferric Compounds / Q000737:chemistry; D006634:Histamine H1 Antagonists / Q000032:analysis*; D008468:Meclizine / Q000032:analysis*; D011736:Pyridoxine / Q000032:analysis*; D011786:Quality Control; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods* / Q000592:standards; D013607:Tablets / Q000737:chemistry / Q000592:standards; D014803:Vitamin B Complex / Q000032:analysis*
chemical_list: D002712:Chlorides; D005290:Ferric Compounds; D006634:Histamine H1 Antagonists; D013607:Tablets; D014803:Vitamin B Complex; D008468:Meclizine; D011736:Pyridoxine; C024555:ferric chloride