title: Association of serum cytokine concentrations with psychological symptoms in midlife women.
abstract: OBJECTIVE
  The purpose of the present study was to clarify the association of serum cytokine concentrations, determined using a multiplexed cytokine assay, with psychological symptoms in midlife women.
  
  
  METHODS
  Fifty-three peri- and post-menopausal women with and without psychological symptoms in Greene's climacteric scale were enrolled in this study. Levels of 17 cytokines in serum samples were measured simultaneously using a multiplexed human cytokine assay.
  
  
  RESULTS
  Serum interleukin (IL)-6 concentration in women with psychological symptoms (2.71+/-047 pg/ml) was significantly (p=0.009) higher than that in women without psychological symptoms (0.98+/-0.18 pg/ml). Serum IL-8 concentration in women with psychological symptoms (33.4+/-8.17 pg/ml) was also significantly (p=0.022) higher than that in women without psychological symptoms (7.87+/-1.64 pg/ml). In addition, serum IL-10 concentration in women with psychological symptoms (0.74+/-0.26 pg/ml) was significantly (p=0.048) higher than that in women without psychological symptoms (0.07+/-0.04 pg/ml). Tumor necrosis factor (TNF)-alpha in serum was detected only in women with psychological symptoms. Serum IL-2 concentration in women with psychological symptoms tended (p=0.066) to be higher than that in women without psychological symptoms. No significant differences were found between levels of other cytokines in women with and without psychological symptoms.
  
  
  CONCLUSIONS
  Psychological stress manifested as climacteric symptoms in midlife women may be associated with increases in serum concentrations of IL-6, IL-8, IL-10, and TNF-alpha.
authors.affiliation: Department of Obstetrics and Gynecology, Course of Human Development, Human Development and Health Science, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima, Japan. yasui@clin.med.tokushima-u.ac.jp
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Yasui
authors.forename: Masahiko
authors.initials: M
authors.lastname: Maegawa
authors.forename: Junko
authors.initials: J
authors.lastname: Tomita
authors.forename: Yuka
authors.initials: Y
authors.lastname: Miyatani
authors.forename: Masayo
authors.initials: M
authors.lastname: Yamada
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Uemura
authors.forename: Shu-ichi
authors.initials: S
authors.lastname: Ueno
authors.forename: Shusuke
authors.initials: S
authors.lastname: Numata
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Ohmori
authors.forename: Naoko
authors.initials: N
authors.lastname: Tsuchiya
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Yuzurihara
authors.forename: Shuichi
authors.initials: S
authors.lastname: Takeda
authors.forename: Minoru
authors.initials: M
authors.lastname: Irahara
mesh_terms: D016207:Cytokines / Q000097:blood*; D005260:Female; D006801:Humans; D008593:Menopause / Q000097:blood / Q000276:immunology* / Q000523:psychology*; D008875:Middle Aged; D047648:Perimenopause / Q000097:blood / Q000276:immunology / Q000523:psychology; D017698:Postmenopause / Q000097:blood / Q000276:immunology / Q000523:psychology; D013315:Stress, Psychological / Q000097:blood / Q000276:immunology*
chemical_list: D016207:Cytokines