title: High incidence of complex chromosome abnormality in cleavage embryos from patients with repeated implantation failure.
abstract: OBJECTIVE
  To analyze the chromosome abnormalities observed in the course of preimplantation aneuploidy screening using comparative genomic hybridization (CGH) on single blastomeres in relation to maternal age and previous IVF history.
  
  
  METHODS
  Retrospective analytical study.
  
  
  METHODS
  A large IVF unit and the research laboratory of an associated clinical genetics unit.
  
  
  METHODS
  Twenty-eight women referred for aneuploidy screening of cleavage embryos.
  
  
  METHODS
  Blastomere biopsy.
  
  
  METHODS
  The incidence of aneuploidy and complex abnormality in human cleavage embryos.
  
  
  RESULTS
  The incidence in embryos of aneuploidy for one to two chromosomes was significantly increased with advanced maternal age, but was independent of any history of recurrent implantation failure. In comparison, the incidence of complex chromosome abnormality (which involves three or more chromosomes) was independent of maternal age but significantly increased in embryos from patients with a history of recurrent implantation failure.
  
  
  CONCLUSIONS
  The incidence of complex abnormality in healthy cleavage embryos is independent of maternal age but is increased in patients with a history of recurrent implantation failure. These results suggest that the pathology underlying complex abnormality is different from that resulting in aneuploidy of one to two chromosomes but particularly relevant to women with recurrent implantation failure.
authors.affiliation: The Murdoch Childrens Research Institute, University of Melbourne Department of Paediatrics, Royal Children's Hospital, Parkville, Australia. lucille.voullaire@mcri.edu.au <lucille.voullaire@mcri.edu.au>
authors.forename: Lucille
authors.initials: L
authors.lastname: Voullaire
authors.forename: Veronica
authors.initials: V
authors.lastname: Collins
authors.forename: Tracey
authors.initials: T
authors.lastname: Callaghan
authors.forename: John
authors.initials: J
authors.lastname: McBain
authors.forename: Robert
authors.initials: R
authors.lastname: Williamson
authors.forename: Leeanda
authors.initials: L
authors.lastname: Wilton
mesh_terms: D000367:Age Factors; D000782:Aneuploidy; D002869:Chromosome Aberrations; D025063:Chromosome Disorders / Q000453:epidemiology / Q000235:genetics; D002970:Cleavage Stage, Ovum / Q000166:cytology*; D010064:Embryo Implantation / Q000235:genetics*; D020964:Embryo Loss / Q000453:epidemiology / Q000235:genetics*; D004624:Embryo Transfer; D047108:Embryonic Development / Q000235:genetics*; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D015994:Incidence; D012189:Retrospective Studies; D017211:Treatment Failure