title: [Meiotic segregation results of male reciprocal chromosome translocations].
abstract: OBJECTIVE
  To analyze the meiotic segregation results of male reciprocal chromosome translocation by fluorescence in situ hybridization (FISH).
  
  
  METHODS
  Multi-color FISH using 3 combined probes located in any 3 chromosome segments on both sides of two breakpoints was performed on the de-condensed sperm head to analyze the sperm chromosomal contents and segregation patterns.
  
  
  RESULTS
  Four male reciprocal translocation carriers were included in the study, with the karyotypes of 46, XY, t(2;18) (p16; q23); 46, XY, t(4;6) (q34;q21); 46, XY, t(8;13) (q23;q21) and 46, XY, t(4;5) (4q31;5q13), respectively. The results showed that 4 carriers had different proportions of various segregated spermatozoa. The spermatozoa of alternate, adjacent-1, adjacent-2, 3:1, non-disjunction in meiosis II, and 4:0 or diploidy accounted for 27.1%-49.4%, 26.9%-37.6%, 2.7%-15.7%, 8.6%-32.7%, 0.2%-1.9%, and 0.1%-0.4%, respectively.
  
  
  CONCLUSIONS
  For each-reciprocal translocation carrier seems to have a particular meiotic segregation results, FISH analysis on sperm head should be done for each carrier in order to provide an accurate genetic counseling.
authors.affiliation: Reproductive Medicine Center, First Affiliated Hospital, Wenzhou Medical College, Wenzhou, Zhejiang, 325000 PR China. xfhuang@hosp1.ac.cn
authors.forename: Xue-feng
authors.initials: XF
authors.lastname: Huang
authors.forename: Shi-quan
authors.initials: SQ
authors.lastname: Xiao
authors.forename: Qian-jin
authors.initials: QJ
authors.lastname: Fei
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Li-ya
authors.initials: LY
authors.lastname: Zhang
authors.forename: Xu
authors.initials: X
authors.lastname: Yang
authors.forename: Bi-lv
authors.initials: BL
authors.lastname: Ye
mesh_terms: D019457:Chromosome Breakage; D006579:Heterozygote; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D008540:Meiosis / Q000235:genetics*; D013094:Spermatozoa / Q000378:metabolism; D014178:Translocation, Genetic / Q000235:genetics*