title: Using interferon-alpha to block expression of cellular ribosome subunit S24 variant 2 in human fibroblasts inhibits translation of the poliovirus genome.
abstract: Studies about the proteins induced by interferon (IFN-)-alpha stimulation have provided some data on their mechanism of antiviral effect. These proteins were confirmed to contribute to antiviral functions. In this study, IFN-alpha stimulation of human fibroblasts was shown to induce the inhibition of S24 variant 2 (a structural component of the ribosomal small subunit) at the mRNA and protein levels, implying a possible antiviral mechanism for IFN-alpha in human fibroblasts. The delay of poliovirus replication by IFN-alpha was partially compensated for by S24 variant 2 expressed in pcDNA vector-transfected cells, and the interference RNA of S24 variant 2 was able to induce mimetically, to some extent, this poliovirus replication delay. These observations revealed that S24 variant 2 could be involved in the antiviral effects of IFN-alpha in human fibroblasts.
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences, Peking Union Medical College, Kunming, People's Republic of China.
authors.forename: Longding
authors.initials: L
authors.lastname: Liu
authors.forename: Pingzhong
authors.initials: P
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Jiang
authors.forename: Chenghong
authors.initials: C
authors.lastname: Dong
authors.forename: Lichun
authors.initials: L
authors.lastname: Wang
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Che
authors.forename: Hongling
authors.initials: H
authors.lastname: Zhao
authors.forename: Shaozhong
authors.initials: S
authors.lastname: Dong
authors.forename: Qihan
authors.initials: Q
authors.lastname: Li
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D005347:Fibroblasts / Q000821:virology; D016679:Genome, Viral; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology*; D017955:Poliovirus / Q000235:genetics*; D014176:Protein Biosynthesis / Q000187:drug effects*; D034622:RNA Interference; D012269:Ribosomal Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics
chemical_list: D000998:Antiviral Agents; D016898:Interferon-alpha; C489053:RPS24 protein, human; D012269:Ribosomal Proteins