Antiapoptotic activity of bovine herpesvirus type-1 (BHV-1) UL14 protein.
Viruses have evolved different strategies to interfere with apoptotic pathways in order to halt cellular responses to infection. One previous study showed that transient transfection of bovine herpesvirus type-1 (BHV-1) UL14 protein is efficient in protecting Madin Darby kidney (MDBK) and human chronic myelogenous leukemia (K562) cells from sorbitol-induced apoptosis. This protein corresponds to a putative protein of BHV-1, which shares aminoacid sequence with a part of the peptide-binding domain conserved in human heat shock protein (HSP70) family. The pBK-CMV-UL14 plasmid transfected MDBK cells treated with sorbitol did not show caspase-3 and caspase-9 activation with respect to non-transfected MDBK cells (UL14 negative). Furthermore, we report that the expression of the full length sequence of BHV-1 UL14 is evident after 7 h of infection of BHV-1 on MDBK cells which were then treated with sorbitol. These results indicate that UL14 gene product has important implications to enhance cell survival in response to apoptotic stimuli.
De Martino
L
L
Department of Pathology and Animal Health, Infectious Diseases, Medicine Veterinary School, University of Naples Federico II, Via F. Delpino 1, 80137 Naples, Italy. luisa.demartino@unina.it
Marfe
G
G
Consalvo
M Irno
MI
Di Stefano
C
C
Pagnini
U
U
Sinibaldi-Salimei
P
P
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D002417:Cattle; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D007242:Herpesvirus 1, Bovine / Q000378:metabolism*; D006801:Humans; D013012:Sorbitol; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
C402639:UL14 protein, Human herpesvirus 1; D014764:Viral Proteins; D013012:Sorbitol; D053148:Caspase 3; D053453:Caspase 9