title: Variables influencing interactions of untargeted quantum dot nanoparticles with skin cells and identification of biochemical modulators.
abstract: Skin cells (NHEK) take up untargeted quantum dots (QD) with surface polyethylene glycol (PEG), amines, and carboxylic acids, but the mechanisms are unknown. Time courses of QD-NHEK interactions were determined and effects of QD surface coating, temperature, culture medium supplements and inhibitors of the cell cycle and endocytosis identified. The magnitude of QD-NHEK interactions was coating dependent. Low-temperature or unsupplemented medium decreased QD-NHEK interactions. Biochemical inhibitors were identified that attenuate and potentiate QD-NHEK interactions. These results are important for understanding and controlling interactions of untargeted QD with cells.
authors.affiliation: Center for Chemical Toxicology Research and Pharmacokinetics, North Carolina State University, 4700 Hillsborough Street, Raleigh, North Carolina 27606, USA.
authors.forename: Jessica P
authors.initials: JP
authors.lastname: Ryman-Rasmussen
authors.forename: Jim E
authors.initials: JE
authors.lastname: Riviere
authors.forename: Nancy A
authors.initials: NA
authors.lastname: Monteiro-Riviere
mesh_terms: D000588:Amines / Q000378:metabolism; D002264:Carboxylic Acids / Q000378:metabolism; D002478:Cells, Cultured; D053758:Nanoparticles; D011092:Polyethylene Glycols / Q000378:metabolism; D045663:Quantum Dots; D012867:Skin / Q000166:cytology / Q000378:metabolism*
chemical_list: D000588:Amines; D002264:Carboxylic Acids; D011092:Polyethylene Glycols