title: Macrophage Serum-Based Adhesion to Plasma-Processed Surface Chemistry is Distinct from That Exhibited by Fibroblasts.
abstract: Plasma-polymerized films deposited from AlAm, HxAm, NVP, NVFA, AA and FC were compared to TCPS and PS surfaces in supporting cellular attachment, viability, and proliferation in serum-based culture in vitro for extended periods of time (>7 d). Surface patterns were created using multi-step depositions with physical masks. Cell adhesion in the presence of serum was compared for (monocyte-) macrophage and fibroblast cell lines. Cellular response was tracked over time, reporting adhesive behavior, proliferative rates, and morphological changes as a function of surface chemistry. Micropatterned surfaces containing different surface chemistries and functional groups (e.g. -NH(2), -COOH, -CF(3)) produced differential cell adhesive patterns for NIH 3T3 fibroblasts compared to J774A.1, RAW 264.7 or IC-21 (monocyte-) macrophage cell types. Significantly, macrophage adhesion is substantial on surfaces where fibroblasts do not adhere under identical culture conditions.
authors.affiliation: Cell and Molecular Biology Program, Colorado State University, Fort Collins, CO 80523-1872, USA.
authors.forename: Marisha L
authors.initials: ML
authors.lastname: Godek
authors.forename: Galiya Sh
authors.initials: GS
authors.lastname: Malkov
authors.forename: Ellen R
authors.initials: ER
authors.lastname: Fisher
authors.forename: David W
authors.initials: DW
authors.lastname: Grainger