Expression analysis of the TAB2 protein in adult mouse tissues.
BACKGROUND
The Interleukin-1 (IL-1) signaling component TAK1 binding protein 2 (TAB2) plays a role in activating the NFkappaB and JNK signaling pathways. Additionally, TAB2 functions in the nucleus as a repressor of NFkappaB-mediated gene regulation.


OBJECTIVE
To obtain insight into the function of TAB2 in the adult mouse, we analyzed the in vivo TAB2 expression pattern.


METHODS
Cell lines and adult mouse tissues were analyzed for TAB2 protein expression and localization.


RESULTS
Immunohistochemical staining for TAB2 protein revealed expression in the vascular endothelium of most tissues, hematopoietic cells and brain cells. While TAB2 is localized in both the nucleus and the cytoplasm in cell lines, cytoplasmic localization predominates in hematopoietic tissues in vivo.


CONCLUSIONS
The TAB2 expression pattern shows striking similarities with previously reported IL-1 receptor expression and NFkappaB activation patterns, suggesting that TAB2 in vivo is playing a role in these signaling pathways.
Orelio
C
C
Erasmus University Medical Centre, Dept. of Cell Biology and Genetics, P.O. Box 1738, 3000 DR Rotterdam, The Netherlands.
Dzierzak
E
E
D048868:Adaptor Proteins, Signal Transducing / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D000375:Aging / Q000502:physiology*; D000818:Animals; D001853:Bone Marrow / Q000378:metabolism; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D006224:Cricetinae; D003593:Cytoplasm / Q000378:metabolism; D005786:Gene Expression Regulation; D007527:Isoenzymes / Q000145:classification / Q000235:genetics / Q000378:metabolism; D020930:MAP Kinase Kinase Kinases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D009928:Organ Specificity; D012333:RNA, Messenger / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013154:Spleen / Q000378:metabolism; D000099151:MAP Kinase Kinase Kinase 7
D048868:Adaptor Proteins, Signal Transducing; D007527:Isoenzymes; D020930:MAP Kinase Kinase Kinases; D012333:RNA, Messenger; D011994:Recombinant Proteins; D000099151:MAP Kinase Kinase Kinase 7; C498761:Tab2 protein, mouse