Glaziovianin A, a new isoflavone, from the leaves of Ateleia glazioviana and its cytotoxic activity against human cancer cells.
A new isoflavone, named glaziovianin A (1), was isolated from the leaves of Ateleia glazioviana (Legminosae) by means of a cytotoxicity-guided fractionation procedure against HL-60 leukemia cells. The chemical structure of 1 was determined by analysis of its extensive spectroscopic data. Glaziovianin A (1) displayed differential cytotoxicities in the Japanese Foundation for Cancer Research 39 cell line panel assay. The pattern of the differential cytotoxicities of 1 was found to correlate to that shown by TZT-1027, suggesting that 1 inhibited tubulin polymerization as an action mechanism. Although 1 had little influence on microtubule networks in interphase cells, 1-treated cells showed abnormal structures with unaligned chromosomes.
Yokosuka
Akihito
A
Laboratory of Medicinal Pharmacognosy, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1, Horinouchi, Hachioji, Tokyo 192-0392, Japan. yokosuka@ps.toyaku.ac.jp
Haraguchi
Mitsue
M
Usui
Takeo
T
Kazami
Sayaka
S
Osada
Hiroyuki
H
Yamori
Takao
T
Mimaki
Yoshihiro
Y
D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D007887:Fabaceae / Q000737:chemistry; D018922:HL-60 Cells; D006801:Humans; D007529:Isoflavones / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D009369:Neoplasms / Q000378:metabolism; D009842:Oligopeptides / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D014404:Tubulin / Q000187:drug effects* / Q000378:metabolism
D000970:Antineoplastic Agents; D007529:Isoflavones; D009842:Oligopeptides; D014404:Tubulin; C521560:glaziovianin A; C105791:soblidotin