title: Effect of insufficient insulin treatment in streptozotocin-induced diabetes mellitus.
abstract: OBJECTIVE
  In this study, we investigated clinically, pathologically, and immunohistochemically the effect of insufficient short-acting insulin treatment on streptozotocin (STZ)-induced diabetes mellitus in rats.
  
  
  METHODS
  Three groups composed of 10 rats each were studied as follows: (1) a group that received only STZ (50 mg/kg) (STZ group); (2) a group that received 50 mg/kg STZ and, after 12 hours, 8 IU of short-acting insulin treatment (STZ + INS group), repeated every night for 5 days; and (3) a control group. Ketonuria and blood glucose levels were examined every day. Blood was obtained from 2 rats from each group, and necropsy was performed every day during the 5-day period.
  
  
  RESULTS
  Hyperglycemia was observed in the STZ and STZ + INS groups 24 hours after, but levels were higher in the STS + INS group than those in the STZ-only group. Histopathology was similar in the STZ and STZ + INS groups, and degeneration was observed in both groups, but immunohistochemistry revealed a more severe reduction in insulin-secreting cells in the STZ + INS group than that in the STZ group. There were no hyperglycemia and histopathological or immunochemical alteration in the control group.
  
  
  CONCLUSIONS
  This study showed that insufficient short-acting insulin treatment can increase the diabetogenic effect of STZ in rats.
authors.affiliation: Department of Pathology, Faculty of Veterinary Medicine, University of Mehmet Akif Ersoy, Burdur, Turkey. oozmen@akdeniz.edu.tr
authors.forename: Ozlem
authors.initials: O
authors.lastname: Ozmen
authors.forename: Senay
authors.initials: S
authors.lastname: Topsakal
authors.forename: Sima
authors.initials: S
authors.lastname: Sahinduran
authors.forename: Mahiye
authors.initials: M
authors.lastname: Ozcelik
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D005260:Female; D006943:Hyperglycemia / Q000453:epidemiology*; D007328:Insulin / Q000627:therapeutic use*; D007515:Islets of Langerhans / Q000473:pathology; D010179:Pancreas / Q000473:pathology; D011384:Proinsulin / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007328:Insulin; D011384:Proinsulin