Large-scale high-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method.
Here, we describe a Library screen transformation protocol using the lithium acetate/single-stranded carrier DNA/PEG method of transformation for Saccharomyces cerevisiae. This method is suitable for screening complex plasmid libraries such as those used for yeast two-hybrid analysis. This procedure takes up to 2.5 h to complete once the yeast culture has been grown.
Gietz
R Daniel
RD
Department of Biochemistry and Medical Genetics, University of Manitoba, T250-770 Bannatyne Ave., Winnipeg, Manitoba R3E 0W3, Canada.
Schiestl
Robert H
RH
D000085:Acetates; D004277:DNA, Single-Stranded / Q000235:genetics; D015723:Gene Library; D005821:Genetic Techniques; D006358:Hot Temperature; D010957:Plasmids; D011092:Polyethylene Glycols; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics*; D014170:Transformation, Genetic
D000085:Acetates; D004277:DNA, Single-Stranded; D011092:Polyethylene Glycols; C488804:lithium acetate