Optimization of in-situ monolithic synthesis for immunopreconcentration in capillary.
Poly(glycidyl methacrylate-co-ethylene dimethacrylate) monoliths have been synthetized in fused-silica capillary. The monomer mixture composition, initiation mode and porogen composition were optimized in order to provide a monolith with an homogeneous morphology and able to generate an electroosmotic flow via the incorporation of a small percentage of monomers possessing sulfonate group. Anti-ochratoxin A antibodies were immobilized through a single step on the epoxy groups leading to a miniaturized immunoextraction column. In order to evaluate the specificity of the analyte-antigen interaction on this immunosorbent, the retention of ochratoxin A was examined on this support but also on two complementary sorbents: one constituted by the non-bonded monolith and another one bonded with non-specific antibodies. Only the monolith bonded with anti-ochratoxin A antibodies lead to retention, showing the specificity of the interactions involved. This affinity phase based on a monolithic polymer support exhibits a high potential for specific preconcentration of small molecules.
Faure
Karine
K
Laboratoire des Sciences Analytiques, UMR 5180 CNRS-Université Lyon 1, Bât CPE, 43 boulevard du 11 novembre 1918, 69622 Villeurbanne Cedex, France.
Delaunay
Nathalie
N
Alloncle
Guillaume
G
Cotte
Stéphane
S
Rocca
Jean-Louis
JL
D000906:Antibodies / Q000276:immunology; D019075:Electrophoresis, Capillary / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D009793:Ochratoxins / Q000276:immunology
D000906:Antibodies; D009793:Ochratoxins; C025589:ochratoxin A