A new highly effective anticysticercosis vaccine expressed in transgenic papaya.
The use of transgenic plants as new antigen-delivery systems for subunit vaccines has been increasingly explored. We herein report progress toward a papaya-based vaccine against cysticercosis. Synthetic peptides (KETc1, KETc12, KETc7) were successfully expressed in 19 different transgenic papaya clones and found to be immunogenic. Complete protection against cysticercosis was induced with the soluble extract of the clones that expressed the higher levels of transcripts in up to 90% of the immunized mice. This study represents a key step towards the development of a more effective, sustainable and affordable oral subunit vaccine against human and pig cysticercosis.
Hernández
Marisela
M
Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México, D.F. 04510, México.
Cabrera-Ponce
José Luis
JL
Fragoso
Gladis
G
López-Casillas
Fernando
F
Guevara-García
Arturo
A
Rosas
Gabriela
G
León-Ramírez
Claudia
C
Juárez
Patricia
P
Sánchez-García
Guadalupe
G
Cervantes
Jaquelynne
J
Acero
Gonzalo
G
Toledo
Andrea
A
Cruz
Carmen
C
Bojalil
Rafael
R
Herrera-Estrella
Luis
L
Sciutto
Edda
E
D000818:Animals; D029441:Carica / Q000235:genetics*; D003551:Cysticercosis / Q000276:immunology* / Q000517:prevention & control*; D003552:Cysticercus / Q000254:growth & development / Q000276:immunology*; D005260:Female; D008018:Life Cycle Stages; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D010529:Peritoneal Cavity / Q000469:parasitology; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D022223:Vaccines, Subunit / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification
D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic