Flagellar motility and structure in the hyperthermoacidophilic archaeon Sulfolobus solfataricus.
Flagellation in archaea is widespread and is involved in swimming motility. Here, we demonstrate that the structural flagellin gene from the crenarchaeaon Sulfolobus solfataricus is highly expressed in stationary-phase-grown cells and under unfavorable nutritional conditions. A mutant in a flagellar auxiliary gene, flaJ, was found to be nonmotile. Electron microscopic imaging of the flagellum indicates that the filaments are composed of right-handed helices.
Szabó
Zalán
Z
Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands.
Sani
Musa
M
Groeneveld
Maarten
M
Zolghadr
Benham
B
Schelert
James
J
Albers
Sonja-Verena
SV
Blum
Paul
P
Boekema
Egbert J
EJ
Driessen
Arnold J M
AJ
D019843:Archaeal Proteins / Q000235:genetics*; D015152:Blotting, Northern; D005407:Flagella / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D005408:Flagellin / Q000235:genetics*; D019848:Gene Expression Regulation, Archaeal; D023061:Gene Order; D046529:Microscopy, Electron, Transmission; D009154:Mutation; D009876:Operon; D048229:Sulfolobus solfataricus / Q000235:genetics* / Q000502:physiology / Q000648:ultrastructure
D019843:Archaeal Proteins; D005408:Flagellin