Colocalization of a partially dominant gene for yellow seed colour with a major QTL influencing acid detergent fibre (ADF) content in different crosses of oilseed rape (Brassica napus).
Quantitative trait loci (QTLs) contributing to yellow seed colour and acid detergent fibre (ADF) were localized and compared in 3 mapping populations developed from 2 crosses (designated 'YE1' and 'YE2') between 2 distinct sources of true-breeding yellow-seeded oilseed rape (Brassica napus) and 2 different black-seeded genotypes. A clear correlation was observed between seed colour and ADF content in both crosses. In all 3 populations, a major QTL, with a large effect on both seed colour and ADF in multiple environments, was detected at the same position on chromosome N18. In YE1, a second minor QTL, with a small effect on seed colour but not on ADF content, was localized on chromosome N1. In YE2, no QTL was observed on N1; however, 2 minor seed-colour loci were localized to N15 and N5. A second major QTL for ADF was localized in YE1 on N13; in YE2, no other QTLs for ADF were detected. Combined QTL and segregation data for seed colour and ADF content in the different populations suggest that a partially dominant B. napus gene for seed colour on N18 contributes to a reduction in fibre content in different yellow-seeded B. napus genotypes. The other QTLs that were identified appear to represent different genes in the 2 yellow-seeded rapeseed sources, which, in each case, affect only fibre content or seed colour, respectively. Potential candidate genes and implications for marker-assisted breeding of oilseed rape with reduced seed dietary fibre content are discussed.
Badani
Ana Gloria
AG
Department of Plant Breeding, Research Centre for BioSystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany.
Snowdon
Rod J
RJ
Wittkop
Benjamin
B
Lipsa
Florin D
FD
Baetzel
Roland
R
Horn
Renate
R
De Haro
Antonio
A
Font
Rafael
R
Lühs
Wilfred
W
Friedt
Wolfgang
W
D029688:Brassica napus / Q000737:chemistry / Q000235:genetics*; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D003433:Crosses, Genetic; D004043:Dietary Fiber / Q000032:analysis; D005799:Genes, Dominant; D018598:Minisatellite Repeats; D010858:Pigmentation / Q000235:genetics*; D010940:Plant Proteins / Q000032:analysis* / Q000235:genetics*; D040641:Quantitative Trait Loci; D012639:Seeds / Q000235:genetics
D004043:Dietary Fiber; D010940:Plant Proteins