title: Villoglandular adenocarcinoma of the cervix: two new cases with morphological and molecular study.
abstract: In this paper, we report 2 new cases of villoglandular papillary adenocarcinoma (VGPA) of the cervix, a rare, well-differentiated form of cervical adenocarcinoma. Both patients were without medical complications or history of oral contraceptive use and were nonsmokers. Extended hysterectomy was performed in both cases. Morphological criteria for a correct pathological diagnosis were emphasized. Immunohistochemical analysis was performed to clarify the phenotype of the neoplasms. Moreover, for the first time, we probed to establish if VGPA could be correlated to human papilloma virus (HPV) and herpes virus (HSV) types 1 and 2, using polymerase chain reaction amplification of tumoral DNA. Both neoplasms showed positivity for B72.3, Ca-125, carcinoembryonic antigen, keratin 7, and p16(INK4a) protein. Vimentin, P53, estrogen, and progesterone receptors, instead, were negative. Molecular study by polymerase chain reaction amplification of tumor DNA revealed a strong positive signal for HPV-DNA and no signal for HSV-DNA. It is reasonable to conclude that our cases of VGPA, in accordance with other examples reported in literature, are due to HPV infection. Behavioral cofactors, such as HSV infection (types 1 and 2), oral contraceptive use and smoking, involved in the pathogenesis of other cervical malignancies, can be excluded for the present cases.
authors.affiliation: Department of Pathology and Medicine of Laboratory, Parma University, Italy. giovanna.giordano@unipr.it
authors.forename: Giovanna
authors.initials: G
authors.lastname: Giordano
authors.forename: Tizsiana
authors.initials: T
authors.lastname: D'Adda
authors.forename: Letizia
authors.initials: L
authors.lastname: Gnetti
authors.forename: Carla
authors.initials: C
authors.lastname: Merisio
authors.forename: Marzio
authors.initials: M
authors.lastname: Gabrielli
authors.forename: Mauro
authors.initials: M
authors.lastname: Melpignano
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis* / Q000473:pathology* / Q000821:virology; D000328:Adult; D000912:Antibodies, Neoplasm / Q000378:metabolism; D018394:CA-125 Antigen / Q000378:metabolism; D002272:Carcinoembryonic Antigen / Q000378:metabolism; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism; D004279:DNA, Viral / Q000235:genetics; D003937:Diagnosis, Differential; D005260:Female; D052162:Human papillomavirus 16 / Q000235:genetics; D006801:Humans; D053552:Keratin-7 / Q000378:metabolism; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000150:complications / Q000235:genetics; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000473:pathology* / Q000821:virology
chemical_list: D000912:Antibodies, Neoplasm; C091202:B72.3 antibody; D018394:CA-125 Antigen; D002272:Carcinoembryonic Antigen; D019941:Cyclin-Dependent Kinase Inhibitor p16; D004279:DNA, Viral; D053552:Keratin-7