title: Accumulation of vitamin E in potato (Solanum tuberosum) tubers.
abstract: Vitamin E (tocopherol) is a powerful antioxidant essential for human health and synthesized only by photosynthetic organisms. The effects of over-expression of tocopherol biosynthetic enzymes have been studied in leaves and seeds, but not in a non-photosynthetic, below-ground plant organ. Genetic and molecular approaches were used to determine if increased levels of tocopherols can be accumulated in potato (Solanum tuberosum L.) tubers through metabolic engineering. Two transgenes were constitutively over-expressed in potato: Arabidopsis thaliana p-hydroxyphenylpyruvate dioxygenase (At-HPPD) and A. thaliana homogentisate phytyltransferase (At-HPT). alpha-Tocopherol levels in the transgenic plants were determined by high-performance liquid chromatography. In potato tubers, over-expression of At-HPPD resulted in a maximum 266% increase in alpha-tocopherol, and over-expression of At-HPT yielded a 106% increase. However, tubers from transgenic plants still accumulated approximately 10- and 100-fold less alpha-tocopherol than leaves or seeds, respectively. The results indicate that physiological and regulatory constraints may be the most limiting factors for tocopherol accumulation in potato tubers. Studying regulation and induction of tocopherol biosynthesis should reveal approaches to more effectively engineer crops with enhanced tocopherol content.
authors.affiliation: Department of Crop and Soil Sciences, Michigan State University, East Lansing, MI 48824, USA. Elizabeth.Crowell@versailles.inra.fr
authors.forename: Elizabeth F
authors.initials: EF
authors.lastname: Crowell
authors.forename: J Mitchell
authors.initials: JM
authors.lastname: McGrath
authors.forename: David S
authors.initials: DS
authors.lastname: Douches
mesh_terms: D015111:4-Hydroxyphenylpyruvate Dioxygenase / Q000235:genetics*; D019883:Alkyl and Aryl Transferases / Q000235:genetics*; D017360:Arabidopsis / Q000201:enzymology*; D015139:Blotting, Southern; D002851:Chromatography, High Pressure Liquid; D017931:DNA Primers; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D017343:Genes, Plant; D018515:Plant Leaves / Q000737:chemistry / Q000201:enzymology; D035281:Plant Tubers / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000737:chemistry / Q000201:enzymology; D011198:Solanum tuberosum / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D019076:Transgenes / Q000502:physiology; D014810:Vitamin E / Q000096:biosynthesis*
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D014810:Vitamin E; D015111:4-Hydroxyphenylpyruvate Dioxygenase; D019883:Alkyl and Aryl Transferases; C412723:phytyltransferase