[Allogenic islet transplantation on the rat liver after allogenic nonparenchymal cells injection in the thymus].
BACKGROUND
[corrected] The major indication for pancreas or islet transplantation is diabetes mellitus type I. This process has to supply the insulin necessity keeping glucose under control


OBJECTIVE
We studied allogenic islet transplantation on the rat liver, Wistar (RT1u) to Lewis (RT1(1)) as a recipient. Control group (n = 8) and nonparenchymal cell group (n = 8) respectively with injection of Hanks solution and nonparenchymal cells in the thymus before islet transplantation.


METHODS
With the method of isolation and purification of the islets we obtained both in the control group 3.637 +/-783,3 islets with purity of 85 +/- 3,52% and nonparenchymal cell group 3.270 +/- 770 islets with purity of 84,25 +/- 2,76%. The nonparenchymal cells were retrieved from the liver and we obtained 2 x 106 cells. Diabetes was induced by i.v. streptozotocin


RESULTS
Control group the transplantation of 3.637 +/- 783,3 islets in the rat liver normalized glucose test, 7,21 +/- 0,57 mmol/L in the 2nd postoperative day. Acute rejection came in the 6th postoperative day with significantly increase of glucose test in nonparenchymal cell group, the transplantation of 3.270 +/- 770 islets in the rat liver, almost normalized the glucose test was 17,95 +/- 5,33 mmol/L in the 2nd postoperative day. From the 4th postoperative day to 10th postoperative day. The glucose test increase significantly showing an early acute rejection


CONCLUSIONS
The injection of nonparenchymal cells in the thymus before allogenic islet transplantation in the rat liver lead to an early acute rejection.
Chaib
Eleazar
E
Departamento de Cirurgia da Universidade de Cambridge, Inglaterra.
Papalois
Apostolos
A
Brons
Ingrid G M
IG
Calne
Roy Y
RY
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002469:Cell Separation / Q000379:methods; D003921:Diabetes Mellitus, Experimental / Q000601:surgery*; D004195:Disease Models, Animal; D005260:Female; D006084:Graft Rejection / Q000276:immunology*; D006085:Graft Survival; D007165:Immunosuppression Therapy / Q000379:methods; D007515:Islets of Langerhans / Q000166:cytology; D016381:Islets of Langerhans Transplantation / Q000276:immunology / Q000379:methods*; D008099:Liver; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D017208:Rats, Wistar; D013311:Streptozocin; D013950:Thymus Gland / Q000276:immunology*
D001786:Blood Glucose; D013311:Streptozocin