title: Immobilization of strictosidine synthase from Catharanthus cell cultures and preparative synthesis of strictosidine.
abstract: Strictosidine synthase was partially purified from Catharanthus roseus cell suspension cultures and immobilized on CNBr-activated Sepharose. The immobilized enzyme exhibits a thermostability increased 300 fold over that of the soluble enzyme and catalyses exclusively the formation of the 3alpha(S)-isomer, strictosidine. Gram quantities of this biologically active glucoalkaloid, which hitherto had been difficult to synthesize and purify, were prepared.
authors.affiliation: Lehrstuhl für Pharmazeutische Biologie, Universität München, Federal Republic of Germany.
authors.forename: U
authors.initials: U
authors.lastname: Pfitzner
authors.forename: M H
authors.initials: MH
authors.lastname: Zenk