title: Posttranslational modifications of transthyretin are serum markers in patients with mycosis fungoides.
abstract: Cutaneous T-cell lymphomas (CTCLs) are characterized by the recruitment of malignant T-cell clones, predominantly of the CD4(+) T-helper subpopulation, into the skin. Mycosis fungoides (MF) is the most common type of CTCL and accounts for almost 50% of all primary cutaneous lymphomas. The ProteinChip technology surface-enhanced laser desorption/ionization time of flight/mass spectrometry (SELDI-TOF-MS) was used to detect biomarkers in sera from MF patients (n = 25) and healthy controls (n = 26). Therefore, diluted sera were applied to IMAC30 ProteinChip arrays, and the resulting protein profiles were bioinformatically analyzed. A protein set that distinguishes MF patients from healthy controls with a sensitivity of 82.6% and a specificity of 100% was identified. Four significant peaks were identified by two-dimensional gel electrophoresis, immunodepletion, and SELDI-TOF-MS as transthyretin (TTR) and three TTR modifications. A subsequent enzyme-linked immunosorbent assay confirmed these findings. The ability to detect and identify proteins and protein modifications using SELDI-TOF-MS might reveal a better insight on this kind of disease and may lead to a better understanding and earlier detection of MF patients.
authors.affiliation: Core Unit Chip Application, Institute of Human Genetics and Anthropology, Medical Faculty, Friedrich-Schiller-University, Jena 07740, Germany.
authors.forename: Niko
authors.initials: N
authors.lastname: Escher
authors.forename: Martin
authors.initials: M
authors.lastname: Kaatz
authors.forename: Christian
authors.initials: C
authors.lastname: Melle
authors.forename: Christina
authors.initials: C
authors.lastname: Hipler
authors.forename: Mirjana
authors.initials: M
authors.lastname: Ziemer
authors.forename: Dominik
authors.initials: D
authors.lastname: Driesch
authors.forename: Uwe
authors.initials: U
authors.lastname: Wollina
authors.forename: Ferdinand
authors.initials: F
authors.lastname: von Eggeling
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood*; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009182:Mycosis Fungoides / Q000097:blood / Q000175:diagnosis*; D011228:Prealbumin / Q000378:metabolism*; D040081:Protein Array Analysis; D011499:Protein Processing, Post-Translational; D012878:Skin Neoplasms / Q000097:blood / Q000175:diagnosis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D014408:Biomarkers, Tumor; D011228:Prealbumin