Silencing gene expression by targeting chromosomal DNA with antigene peptide nucleic acids and duplex RNAs.
The value of recognizing cellular RNA sequences by short interfering RNAs (siRNAs) in mammalian cells is widely appreciated, but what might be learned if it were also possible to recognize chromosomal DNA? Recognition of chromosomal DNA would have many applications, such as inhibiting gene expression, activating gene expression, introducing mutations, and probing chromosome structure and function. We have shown that antigene peptide nucleic acids (agPNAs) and antigene duplex RNAs (agRNAs) block gene expression and probe chromosomal DNA. Here we describe a protocol for designing antigene agents and introducing them into cells. This protocol can also be used to silence expression with PNAs or siRNAs that target mRNA. From preparation of oligomers to analysis of data, experiments with agPNAs and agRNAs require approximately 14 d and 9 d, respectively.
Janowski
Bethany A
BA
Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390-9041, USA. bethany.janowski@utsouthwestern.edu
Hu
Jiaxin
J
Corey
David R
DR
D002875:Chromosomes / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D020868:Gene Silencing; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D012330:RNA, Double-Stranded / Q000737:chemistry*; D024363:Transcription Initiation Site; D014162:Transfection / Q000379:methods*
D020135:Peptide Nucleic Acids; D012330:RNA, Double-Stranded; D004247:DNA