Discovery of tMAC: a Drosophila testis-specific meiotic arrest complex paralogous to Myb-Muv B.
The Drosophila Myb-Muv B (MMB)/dREAM complex regulates gene expression and DNA replication site-specifically, but its activities in vivo have not been thoroughly explored. In ovarian amplification-stage follicle cell nuclei, the largest subunit, Mip130, is a negative regulator of replication, whereas another subunit, Myb, is a positive regulator. Here, we identified a mutation in mip40 and generated a mutation in mip120, two additional MMB subunits. Both mutants were viable, but mip120 mutants had many complex phenotypes including shortened longevity and severe eye defects. mip40 mutant females had severely reduced fertility, whereas mip120 mutant females were sterile, substantiating ovarian regulatory role(s) for MMB. Myb accumulation and binding to polytene chromosomes was dependent on the core factors of the MMB complex. In contrast to the documented mip130 mutant phenotypes, both mip40 and mip120 mutant males were sterile. We purified Mip40-containing complexes from testis nuclear extracts and identified tMAC, a new testis-specific meiotic arrest complex that contained Mip40, Caf1/p55, the Mip130 family member, Always early (Aly), and a Mip120 family member, Tombola (Tomb). Together, these data demonstrate that MMB serves diverse roles in different developmental pathways, and members of MMB can be found in alternative, noninteracting complexes in different cell types.
Beall
Eileen L
EL
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720, USA.
Lewis
Peter W
PW
Bell
Maren
M
Rocha
Michael
M
Jones
D Leanne
DL
Botchan
Michael R
MR
D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000737:chemistry; D002474:Cell-Free System; D002843:Chromatin / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D004330:Drosophila / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D005123:Eye / Q000033:anatomy & histology; D005260:Female; D008297:Male; D008540:Meiosis; D018832:Molecular Chaperones / Q000378:metabolism; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism; D020598:Proto-Oncogene Proteins c-myb / Q000378:metabolism; D012098:Reproduction; D056505:Retinoblastoma-Binding Protein 4; D016415:Sequence Alignment; D013087:Spermatids / Q000166:cytology; D013737:Testis / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
C110251:Caf1-55 protein, Drosophila; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D029721:Drosophila Proteins; C519699:Mip120 protein, Drosophila; C489655:Mip130 protein, Drosophila; C519697:Mip40 protein, Drosophila; D018832:Molecular Chaperones; D046912:Multiprotein Complexes; C467519:Myb protein, Drosophila; D009687:Nuclear Proteins; D020598:Proto-Oncogene Proteins c-myb; D056505:Retinoblastoma-Binding Protein 4; D014157:Transcription Factors; C418886:aly protein, Drosophila; C471972:comr protein, Drosophila; C519698:tomb protein, Drosophila; C486226:topi protein, Drosophila