The combination of peripheral nerve grafts and acidic fibroblast growth factor enhances arginase I and polyamine spermine expression in transected rat spinal cords.
Treatment with a combination of peripheral nerve grafts and acidic fibroblast growth factor improves hind limb locomotor function after spinal cord transection. This study examined the effect of treatment on expression of arginase I (Arg I) and polyamines. Arg I expression was low in the spinal cords of normal rats but increased following spinal injury. Only fully repaired spinal cords expressed higher Arg I levels 6-14 days following repair. In 10-day repaired spinal cords, high Arg I immunoreactivity was detected in motoneurons and alternatively activated macrophages in the graft area and graft-stump edges, and high levels of the polyamine spermine were expressed by macrophages within the intercostal nerve graft. Thus, in addition to enhancing the expression of Arg I and spermine in repaired spinal cords, our treatment may recruit activated macrophages and create a more favorable environment for axonal regrowth.
Kuo
Huai-Sheng
HS
Neural Regeneration Laboratory, Department of Neurosurgery, Neurological Institute, Taipei Veterans General Hospital, Taipei, Taiwan.
Tsai
May J
MJ
Huang
Ming-Chao
MC
Huang
Wen-Cheng
WC
Lee
Meng-Jen
MJ
Kuo
Wen-Chun
WC
You
Li-Hua
LH
Szeto
Ka-Chun
KC
Tsai
I-Lun
IL
Chang
Wen-Chi
WC
Chiu
Chuan-Wen
CW
Ma
Hsu
H
Chak
Kin-Fu
KF
Cheng
Henrich
H
D000818:Animals; D001119:Arginase / Q000378:metabolism*; D002352:Carrier Proteins / Q000008:administration & dosage*; D005260:Female; D008565:Membrane Proteins; D009416:Nerve Regeneration / Q000187:drug effects / Q000502:physiology; D010525:Peripheral Nerves / Q000637:transplantation*; D011073:Polyamines / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013096:Spermine / Q000378:metabolism*; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism / Q000601:surgery; D013119:Spinal Cord Injuries / Q000188:drug therapy / Q000378:metabolism* / Q000601:surgery*
D002352:Carrier Proteins; C116274:FIBP protein, human; D008565:Membrane Proteins; D011073:Polyamines; D013096:Spermine; D001119:Arginase