title: [Role of malnutrition in intestinal anastomosis collagenization: an analysis of procollagen (PINP) and carboxyterminal telopeptide (ICTP) by radioimmunoassay].
abstract: BACKGROUND
  some clinical, anatomo-pathological, and technical factors influence the correct healing of intestinal suture following an intestinal resection. One of the most influential factors is patient nutritional status.
  
  
  OBJECTIVE
  to evaluate the influence of malnutrition on the viability of primary intestinal anastomosis by the analysis of collagen I deposition.
  
  
  METHODS
  40 Wistar rats, radioimmunoassay material. We used 2 groups of rats, 20 animals in each group: a control group (A) and a "malnutrition" group (B).
  
  
  RESULTS
  there was a decrease in PINP (procollagen) deposition in the colon of group B rats as compared to the colon of group A (0.3620 and 0.4340 mg/g respectively) (p = 0.032). There is an increase in ICTP (carboxyterminal telopeptide) in the colon of group B (0.9545 as against 0.8460 mg/g in group A) (p = 0.875). In anastomoses of group B there was a decrease in PINP synthesis as compared to group A (0.376 and 0.468 mg/g respectively, p = 0.002). As regards ICTP, there was an increase in group B (p = 0.330). In relation to the control group no differences were observed in ICTP increases in group B (p = 1).
  
  
  CONCLUSIONS
  colonic anastomosis increases the levels of PINP and ICTP in healed tissue (p = 0.000); malnutrition reduces collagenization in anastomoses (p = 0.000).
authors.affiliation: Servicio de Cirugía General y de Aparato Digestivo, Hospitales Universitarios Virgen del Rocío, Sevilla.
authors.forename: J M
authors.initials: JM
authors.lastname: Alamo
authors.forename: A
authors.initials: A
authors.lastname: Galindo
authors.forename: S
authors.initials: S
authors.lastname: Morales
authors.forename: G
authors.initials: G
authors.lastname: Daza
authors.forename: M
authors.initials: M
authors.lastname: Socas
authors.forename: G
authors.initials: G
authors.lastname: Suárez-Artacho
authors.forename: J M
authors.initials: JM
authors.lastname: Suárez-Grau
authors.forename: J
authors.initials: J
authors.lastname: García-Moreno
authors.forename: F
authors.initials: F
authors.lastname: Pareja
authors.forename: M A
authors.initials: MA
authors.lastname: Gómez
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D024042:Collagen Type I; D003106:Colon / Q000601:surgery*; D004195:Disease Models, Animal; D005260:Female; D044342:Malnutrition / Q000150:complications*; D009752:Nutritional Status; D010446:Peptide Fragments / Q000032:analysis*; D010455:Peptides; D011347:Procollagen / Q000032:analysis*; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing
chemical_list: D024042:Collagen Type I; D010446:Peptide Fragments; D010455:Peptides; D011347:Procollagen; C052929:collagen type I trimeric cross-linked peptide; C042813:procollagen type I carboxy terminal peptide