Prostaglandin E2 inhibits Paracoccidioides brasiliensis killing by human monocytes.
Human monocytes lacked fungicidal activity against high virulence strain of Paracoccidioides brasiliensis, even after IFN-gamma activation. However, monocytes treated with indomethacin exhibited an effective killing against this fungus, suggesting a role of prostaglandin E2 (PGE2) in the inhibition process. Thus, the purpose of this work was to determine whether the effect of PGE2 in fungicidal activity was related with decrease on H(2)O(2) release, the metabolite involved in P. brasiliensis killing, and changes in the levels of TNF-alpha, IL-6 and IL-10. Human monocytes challenged with the fungus produced high PGE2 levels, which in turn inhibited the fungicidal activity of these cells by reducing H(2)O(2) and TNF-alpha production.
Bordon
Ana Paula
AP
Department of Microbiology and Immunology, Institute of Biosciences, São Paulo State University - UNESP, Botucatu, CEP: 18618-000 Sao Paulo, Brazil.
Dias-Melicio
Luciane Alarcão
LA
Acorci
Michele Janegitz
MJ
Calvi
Sueli Aparecida
SA
Serrão Peraçoli
Maria Terezinha
MT
Victoriano de Campos Soares
Angela Maria
AM
D000328:Adult; D002478:Cells, Cultured; D015232:Dinoprostone / Q000378:metabolism*; D015536:Down-Regulation; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D016753:Interleukin-10 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism*; D010228:Paracoccidioides / Q000378:metabolism*; D010229:Paracoccidioidomycosis / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D006861:Hydrogen Peroxide; D015232:Dinoprostone