Flow cytometry characterization of leukemic phase of nasal NK/T-cell lymphoma in tumor biopsies and peripheral blood.
We report the findings of the immunophenotypic profile of three cases of nasal T/NK cell lymphoma in leukemic phase. Flow cytometry analysis was carried out using cell suspensions of tumor nasal biopsies and peripheral blood. Tumor samples were composed by a mixture of a predominant subset of medium-size true NK cytCD3epsilon-, sCD3epsilon-, CD56+ cells mixed with a minor subset of medium-size T/NK sCD3epsilon+, CD56+ cells. Both subsets were also detected in peripheral blood. In addition, an infiltration of small-size sCD3epsilon+, CD56- normal T lymphocytes was also present.
Falcão
Roberto P
RP
Center for Cell-Based Therapy, Department of Clinical Medicine, School of Medicine-USP, Ribeirão Preto, SP, Brazil.
Rizzatti
Edgar G
EG
Saggioro
Fabiano P
FP
Garcia
Aglair B
AB
Marinato
André F
AF
Rego
Eduardo M
EM
D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis; D001706:Biopsy; D020031:Epstein-Barr Virus Infections / Q000097:blood / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D007694:Killer Cells, Natural / Q000737:chemistry / Q000473:pathology* / Q000821:virology; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood / Q000473:pathology*; D016246:Lymphocytes, Tumor-Infiltrating / Q000737:chemistry / Q000473:pathology*; D008297:Male; D008444:Maxillary Sinus Neoplasms / Q000097:blood / Q000473:pathology; D009303:Nasopharyngeal Neoplasms / Q000097:blood / Q000473:pathology*; D009669:Nose Neoplasms / Q000097:blood / Q000473:pathology*; D054340:Receptors, KIR / Q000032:analysis; D016176:T-Lymphocyte Subsets / Q000737:chemistry / Q000473:pathology*; D014763:Viral Matrix Proteins / Q000032:analysis
D015703:Antigens, CD; D014408:Biomarkers, Tumor; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D054340:Receptors, KIR; D014763:Viral Matrix Proteins