title: ALCAM (CD166) is a surface marker for early murine cardiomyocytes.
abstract: ALCAM (activated leukocyte cell adhesion molecule, CD166) belongs to the immunoglobulin superfamily and is involved in axon guidance, hematopoiesis, immune response and tumor metastasis. During embryogenesis, mRNA encoding ALCAM was expressed in the cardiac crescent and the neural groove at embryonic day (E) 7.75 and predominately in the tubular heart at E8.5. A newly generated monoclonal antibody against the ALCAM molecule (ALC-48) exclusively stained cardiomyocytes at E8.25-10.5. However, ALCAM expression was lost by cardiomyocytes by E12.5 and its expression shifts to a variety of organs during later stages. ALCAM was found to be a prominent surface marker for cardiomyocytes in early embryonic hearts. The transient expression of ALCAM during early developmental stages marks specific developmental stages in cardiomyocyte differentiation.
authors.affiliation: Foundation of Biomedical Research and Innovation, Kobe, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Hirata
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Murakami
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Miyamoto
authors.forename: Mako
authors.initials: M
authors.lastname: Tosaka
authors.forename: Kayoko
authors.initials: K
authors.lastname: Inoue
authors.forename: Ayako
authors.initials: A
authors.lastname: Nagahashi
authors.forename: Lars Martin
authors.initials: LM
authors.lastname: Jakt
authors.forename: Takayuki
authors.initials: T
authors.lastname: Asahara
authors.forename: Hiroo
authors.initials: H
authors.lastname: Iwata
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Sawa
authors.forename: Shin
authors.initials: S
authors.lastname: Kawamata
mesh_terms: D020410:Activated-Leukocyte Cell Adhesion Molecule / Q000032:analysis*; D000818:Animals; D000911:Antibodies, Monoclonal; D015415:Biomarkers / Q000032:analysis; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure; D005318:Fetal Heart / Q000166:cytology; D005333:Fetus; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney; D051379:Mice; D032342:Muscle Cells / Q000166:cytology*; D009206:Myocardium / Q000166:cytology*
chemical_list: D020410:Activated-Leukocyte Cell Adhesion Molecule; D000911:Antibodies, Monoclonal; D015415:Biomarkers