Synergistic action of polyanionic and non-polar cofactors in fibrillation of human islet amyloid polypeptide.
Recent studies have led us to suppose that synergistic action of multiple solute cofactors could play substantial roles in amyloid-type fibrillogenesis of pathogenic polypeptides. To support this view, we performed aggregation experiments of human islet amyloid polypeptide (IAPP) in media containing both polyanions and non-polar solvents. The results demonstrated that the fibrillation at sub-micromolar IAPP occurred only when polyanionic and non-polar solutes coexist. A simple sum of two independent cofactor's effects could not account for the synergistic action. We propose that this synergy of polyanionic and nonpolar milieus could substantially modify the amyloidgenesis in the human body.
Konno
Takashi
T
Department of Molecular Physiology and Biophysics, Faculty of Medical Sciences, University of Fukui, Matsuoka, Yoshida, Fukui 910-1193, Japan. konno@fmsrsa.fukui-med.ac.jp <konno@fmsrsa.fukui-med.ac.jp>
Oiki
Shigetoshi
S
Morii
Takashi
T
D000438:Alcohols / Q000737:chemistry; D000682:Amyloid / Q000737:chemistry / Q000648:ultrastructure*; D006025:Glycosaminoglycans / Q000737:chemistry; D006493:Heparin / Q000737:chemistry; D006801:Humans; D058228:Islet Amyloid Polypeptide; D000071228:Polyelectrolytes; D011108:Polymers / Q000737:chemistry*; D011138:Polytetrafluoroethylene / Q000737:chemistry; D011498:Protein Precursors / Q000737:chemistry / Q000648:ultrastructure*; D012996:Solutions / Q000737:chemistry
D000438:Alcohols; D000682:Amyloid; D006025:Glycosaminoglycans; D006493:Heparin; D058228:Islet Amyloid Polypeptide; D000071228:Polyelectrolytes; D011108:Polymers; D011138:Polytetrafluoroethylene; D011498:Protein Precursors; D012996:Solutions