A rodent model of myocardial infarction for testing the efficacy of cells and polymers for myocardial reconstruction.
We have developed a robust rat model of myocardial infarction (MI). Here we describe the step-by-step protocol for creating an ischemia-reperfusion rat model of MI. We also describe how to deliver therapeutic injections of mesenchymal stem cells (MSCs) together with fibrin, to show an application of this model. In addition, to confirm the presence of fibrin and cells in the infarct, visualization of MSCs and fibrin by histological techniques are also described. The ischemia-reperfusion MI model can be modified and generalized for use with various injectable polymers, cell types, drugs, DNA and combinations thereof. The model can be created in 7 days or less, depending on the timing of therapeutic intervention.
Huang
Ngan F
NF
University of California San Francisco and University of California Berkeley Joint Bioengineering Graduate Group, Berkeley, California, 94720-1762 USA.
Sievers
Richard E
RE
Park
Jennifer S
JS
Fang
Qizhi
Q
Li
Song
S
Lee
Randall J
RJ
D000818:Animals; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D004195:Disease Models, Animal; D005337:Fibrin / Q000008:administration & dosage; D006652:Histological Techniques / Q000379:methods*; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D009203:Myocardial Infarction / Q000503:physiopathology*; D051381:Rats; D015427:Reperfusion Injury / Q000469:parasitology* / Q000628:therapy*
D005337:Fibrin