title: Simultaneous clustering of gene expression data with clinical chemistry and pathological evaluations reveals phenotypic prototypes.
abstract: BACKGROUND
  Commonly employed clustering methods for analysis of gene expression data do not directly incorporate phenotypic data about the samples. Furthermore, clustering of samples with known phenotypes is typically performed in an informal fashion. The inability of clustering algorithms to incorporate biological data in the grouping process can limit proper interpretation of the data and its underlying biology.
  
  
  RESULTS
  We present a more formal approach, the modk-prototypes algorithm, for clustering biological samples based on simultaneously considering microarray gene expression data and classes of known phenotypic variables such as clinical chemistry evaluations and histopathologic observations. The strategy involves constructing an objective function with the sum of the squared Euclidean distances for numeric microarray and clinical chemistry data and simple matching for histopathology categorical values in order to measure dissimilarity of the samples. Separate weighting terms are used for microarray, clinical chemistry and histopathology measurements to control the influence of each data domain on the clustering of the samples. The dynamic validity index for numeric data was modified with a category utility measure for determining the number of clusters in the data sets. A cluster's prototype, formed from the mean of the values for numeric features and the mode of the categorical values of all the samples in the group, is representative of the phenotype of the cluster members. The approach is shown to work well with a simulated mixed data set and two real data examples containing numeric and categorical data types. One from a heart disease study and another from acetaminophen (an analgesic) exposure in rat liver that causes centrilobular necrosis.
  
  
  CONCLUSIONS
  The modk-prototypes algorithm partitioned the simulated data into clusters with samples in their respective class group and the heart disease samples into two groups (sick and buff denoting samples having pain type representative of angina and non-angina respectively) with an accuracy of 79%. This is on par with, or better than, the assignment accuracy of the heart disease samples by several well-known and successful clustering algorithms. Following modk-prototypes clustering of the acetaminophen-exposed samples, informative genes from the cluster prototypes were identified that are descriptive of, and phenotypically anchored to, levels of necrosis of the centrilobular region of the rat liver. The biological processes cell growth and/or maintenance, amine metabolism, and stress response were shown to discern between no and moderate levels of acetaminophen-induced centrilobular necrosis. The use of well-known and traditional measurements directly in the clustering provides some guarantee that the resulting clusters will be meaningfully interpretable.
authors.affiliation: National Center for Toxicogenomics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA. bushel@niehs.nih.gov
authors.forename: Pierre R
authors.initials: PR
authors.lastname: Bushel
authors.forename: Russell D
authors.initials: RD
authors.lastname: Wolfinger
authors.forename: Greg
authors.initials: G
authors.lastname: Gibson
mesh_terms: D000082:Acetaminophen / Q000633:toxicity; D000465:Algorithms; D000818:Animals; D019963:Clinical Chemistry Tests / Q000706:statistics & numerical data*; D016000:Cluster Analysis; D003627:Data Interpretation, Statistical; D004195:Disease Models, Animal; D020869:Gene Expression Profiling / Q000706:statistics & numerical data*; D006331:Heart Diseases / Q000097:blood / Q000235:genetics / Q000473:pathology; D006801:Humans; D008099:Liver / Q000187:drug effects / Q000473:pathology; D009336:Necrosis; D010641:Phenotype; D051381:Rats
chemical_list: D000082:Acetaminophen