title: A buoyancy-driven compact thermocycler for rapid PCR.
abstract: The authors have designed a novel convective flow-based thermocycling system capable of performing high-speed DNA amplification via the polymerase chain reaction in a simplified and inexpensive format. Successful amplification of a 191 bp influenza-A target is demonstrated within 25 minutes using a 10 muL reaction volume with no modification to standard laboratory protocols. The system is simple to assemble and can be readily integrated with existing laboratory instrumentation for automated operation.
authors.affiliation: Center for Engineering in Medicine, Department of Surgery, Massachusetts General Hospital, Shriners Burns Hospital and Harvard Medical School, Boston, MA 02114, USA. nagrawal1@partners.org <nagrawal1@partners.org>
authors.forename: Nitin
authors.initials: N
authors.lastname: Agrawal
authors.forename: Victor M
authors.initials: VM
authors.lastname: Ugaz
mesh_terms: D001331:Automation; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006358:Hot Temperature; D009980:Influenza A virus / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods; D013997:Time Factors
chemical_list: D004247:DNA