Expression, crystallization and preliminary crystallographic data analysis of filamin A repeats 14-16.
Human filamin A is a 280 kDa protein involved in actin-filament cross-linking. It is structurally divided into an actin-binding headpiece (ABD) and a rod domain containing 24 immunoglobulin-like (Ig) repeats. A fragment of human filamin A (Ig repeats 14-16) was cloned and expressed in Escherichia coli and the purified protein was crystallized in 1.6 M ammonium sulfate, 2% PEG 1000 and 100 mM HEPES pH 7.5. The crystals diffracted to 1.95 A and belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 50.63, b = 52.10, c = 98.46 A, alpha = beta = gamma = 90 degrees.
Aguda
Adeleke Halilu
AH
Department of Medical Biochemistry and Microbiology, Uppsala University, Sweden. adelekeha@imcb.a-star.edu.sg
Sakwe
Amos Malle
AM
Rask
Lars
L
Robinson
Robert Charles
RC
D001483:Base Sequence; D003001:Cloning, Molecular; D003285:Contractile Proteins / Q000737:chemistry* / Q000235:genetics; D003460:Crystallization; D018360:Crystallography, X-Ray; D017931:DNA Primers; D018076:DNA, Complementary; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D064448:Filamins; D006801:Humans; D008840:Microfilament Proteins / Q000737:chemistry* / Q000235:genetics; D011487:Protein Conformation
D003285:Contractile Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D064448:Filamins; D008840:Microfilament Proteins