A novel type of sequence variation: multiple-nucleotide length polymorphisms discovered in the bovine genome.
Three types of sequence variations--single-nucleotide polymorphisms (SNPs), insertions and deletions (indels), and short tandem repeats (STRs)--have been extensively reported in mammalian genomes. In this study, we discovered a novel type of sequence variation, i.e., multiple-nucleotide length polymorphisms (MNLPs) in bovine UCN3 (Urocortin 3) and its receptor CRHR2 (corticotropin-releasing hormone receptor 2) genes. Both MNLPs featured involvement of multiple-nucleotide length polymorphisms (5-18 bases), low sequence identity, and 1.7- to 11-fold changes in promoter activity between two alleles. Therefore, this novel genetic complexity would contribute significantly to the evolutionary, functional, and phenotypic complexity of genomes within or among species.
Jiang
Zhihua
Z
Department of Animal Science, Washington State University, Pullman, WA 99164-6351, USA. jiangz@wsu.edu
Wang
Zeping
Z
Kunej
Tanja
T
Williams
Galen A
GA
Michal
Jennifer J
JJ
Wu
Xiao-Lin
XL
Magnuson
Nancy S
NS
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002417:Cattle / Q000235:genetics*; D003346:Corticotropin-Releasing Hormone / Q000235:genetics; D005828:Genetics, Population; D016678:Genome / Q000235:genetics*; D006239:Haplotypes; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics; D018019:Receptors, Corticotropin-Releasing Hormone / Q000235:genetics
C101531:CRF receptor type 2; D018019:Receptors, Corticotropin-Releasing Hormone; D003346:Corticotropin-Releasing Hormone