Glutathione S-transferase polymorphisms and oral cancer: a case-control study in Rio de Janeiro, Brazil.
This study evaluates the influence of genetic polymorphisms at GSTM1, GSTT1 and GSTP1 gene loci on oral cancer susceptibility among Brazilians from Rio de Janeiro. DNA extracted from white blood cells of 231 oral cancer patients and 212 hospital controls was analyzed by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) methods. GSTM1 polymorphism distribution was different between cases and controls (P=0.006), with an overrepresentation of GSTM1 A/B genotype in controls. GSTM1 A/B individuals were at decreased oral cancer risk (OR=0.08; 95% CI=0.05-0.62). No statistically significant association was observed for GSTT1 and GSTP1 polymorphisms. Differences in the GSTM1 and GSTT1 null genotype frequencies were observed between individuals of European origin and African origin, but these genotypes do not seem to influence the risk of oral cancer. Therefore, these results do not support the hypothesis of increased risk of GSTP1 G/G, GSTM1 or GSTT1 null genotypes for developing cancer in oral cavity, but the GSTM1 A/B genotype emerged as a protective factor.
Hatagima
Ana
A
Departamento de Genética, Laboratório de Genética Humana - IOC-FIOCRUZ, Pavilhão Leônidas Deane - 6 Andar - Sala 611, Avenida Brasil, 4365 Manguinhos, Rio de Janeiro, Rio de Janeiro 21045-900, Brazil. anah@ioc.fiocruz.br <anah@ioc.fiocruz.br>
Costa
Emmerson C B
EC
Marques
Christiane F S
CF
Koifman
Rosalina J
RJ
Boffetta
Paolo
P
Koifman
Sergio
S
D000328:Adult; D000368:Aged; D001938:Brazil; D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000235:genetics; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D016013:Likelihood Functions; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D009062:Mouth Neoplasms / Q000201:enzymology* / Q000235:genetics; D011110:Polymorphism, Genetic
D005982:Glutathione Transferase; D007527:Isoenzymes; C413545:glutathione S-transferase T1