title: Use of a commercially available reagent for the selective detection of homocysteine in plasma.
abstract: A procedure for the detection of homocysteine (Hcy) in blood plasma is described. A commercially available chromogen is added to the plasma sample. The plasma solution turns from yellow to blue upon heating for 4 min when a detectable threshold level of Hcy is present. Chromatographic separations and immunogenic materials are not needed. The protocol takes approximately 30 min.
authors.affiliation: Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana 70803, USA.
authors.forename: Jorge O
authors.initials: JO
authors.lastname: Escobedo
authors.forename: Weihua
authors.initials: W
authors.lastname: Wang
authors.forename: Robert M
authors.initials: RM
authors.lastname: Strongin
mesh_terms: D002863:Chromogenic Compounds; D006710:Homocysteine / Q000097:blood*; D006801:Humans
chemical_list: D002863:Chromogenic Compounds; D006710:Homocysteine