Role of myofibril-inducing RNA in cardiac TnT expression in developing Mexican axolotl.
The Mexican axolotl, Ambystoma mexicanum, has been a useful animal model to study heart development and cardiac myofibrillogenesis. A naturally-occurring recessive mutant, gene "c", for cardiac non-function in the Mexican axolotl causes a failure of myofibrillogenesis due to a lack of tropomyosin expression in homozygous mutant (c/c) embryonic hearts. Myofibril-inducing RNA (MIR) rescues mutant hearts in vitro by promoting tropomyosin expression and myofibril formation thereafter. We have studied the effect of MIR on the expression of various isoforms of cardiac troponin T (cTnT), a component of the thin filament that binds with tropomyosin. Four alternatively spliced cTnT isoforms have been characterized from developing axolotl heart. The expression of various cTnT isoforms in normal, mutant, and mutant hearts corrected with MIR, is evaluated by real-time RT-PCR using isoform specific primer pairs; MIR affects the total transcription as well as the splicing of the cTnT in axolotl heart.
Sferrazza
Gian Franco
GF
Department of Biomedical Science, Florida Atlantic University, Boca Raton, FL 33431, USA.
Zhang
Chi
C
Jia
Pingping
P
Lemanski
Sharon L
SL
Athauda
Gagani
G
Stassi
Alyssa
A
Halager
Kristine
K
Maier
Jennifer A
JA
Rueda-de-Leon
Elena
E
Gupta
Amit
A
Dube
Syamalima
S
Huang
Xupei
X
Prentice
Howard M
HM
Dube
Dipak K
DK
Lemanski
Larry F
LF
D000558:Ambystoma mexicanum / Q000196:embryology* / Q000235:genetics; D000818:Animals; D030801:Animals, Genetically Modified; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D006321:Heart / Q000196:embryology* / Q000502:physiology; D009206:Myocardium / Q000378:metabolism*; D009210:Myofibrils / Q000502:physiology*; D012313:RNA / Q000235:genetics / Q000378:metabolism*; D013329:Structure-Activity Relationship; D020107:Troponin T / Q000235:genetics* / Q000378:metabolism*
D020107:Troponin T; D012313:RNA