Cytofluorometric analysis of phenotypes of human bone marrow and umbilical fibroblast-like cells.
Comparative analysis of the expression of some surface markers of human bone marrow mesenchymal stem cells, umbilical fibroblast-like cells, and skin fibroblasts was carried out by the flow cytofluorometry method. Mesenchymal stem cells and umbilical fibroblast-like cells were similar by the levels of expression of the main histocompatibility complex antigens, adhesion molecules, and some growth factor receptors. The profile of skin fibroblast surface antigens was characterized by higher expression of the markers typical of differentiated cells. The results prove the possibility of using umbilical fibroblast-like cells as an alternative source of mesenchymal stem cells for cell replacement therapy.
Lupatov
A Yu
AY
V. N. Orekhovich Institute of Biomedical Chemistry, Moscow.
Karalkin
P A
PA
Suzdal'tseva
Yu G
YG
Burunova
V V
VV
Yarygin
V N
VN
Yarygin
K N
KN
D015703:Antigens, CD / Q000032:analysis; D001854:Bone Marrow Cells / Q000166:cytology* / Q000276:immunology; D005312:Fetal Blood / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology* / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods; D006680:HLA Antigens / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology; D010641:Phenotype; D012867:Skin / Q000166:cytology / Q000276:immunology
D015703:Antigens, CD; D006680:HLA Antigens