Trehalose enhanced the freezability of Poodle dog sperm collected by an artificial vagina (AV).
In an attempt to develop a suitable freezing method for Poodle dog sperm, an experiment was conducted to investigate semen collection methods of digital stimulation and an artificial vagina (AV), using Tris and trehalose-egg yolk extender, on the characteristics and cryopreservation of sperm. Two dogs (dogs A and B) were subjected to semen collection by digital stimulation and AV. The volume, sperm concentration, sperm motility index (SMI) and acrosome status of ejaculates were determined immediately after collection. The remainder was frozen as pellets in Tris and trehalose-egg yolk extender. Sperm motility index was evaluated after thawing and during a thermal resistance test, and acrosome integrity was also assessed. No significant differences regarding sperm concentrations, SMI and acrosome integrity were observed between semen collected by AV and digital stimulation. However, when dog sperm were collected by an AV and frozen in trehalose-egg yolk extender, the motility index of frozen-thawed sperm was significantly improved compared to sperm frozen in Tris-egg yolk extender which were collected by digital stimulation. In conclusion, semen collected by an AV and frozen in trehalose-egg yolk extender was effective in enhancing the freezability of Poodle dog sperm.
Yamashiro
Hideaki
H
Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-amamiyamachi, Aoba-ku, Sendai 981-8555, Japan. hyamashiro@bios.tohoku.ac.jp
Narita
Kaori
K
Sugimura
Satoshi
S
Han
Young-Joon
YJ
Sugawara
Atsushi
A
Morohaku
Kanako
K
Nakazato
Fumiaki
F
Konno
Tsutomu
T
Yoshida
Muneyoshi
M
Sato
Eimei
E
D000179:Acrylates / Q000494:pharmacology; D000818:Animals; D019343:Citric Acid / Q000494:pharmacology; D015925:Cryopreservation / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004285:Dogs; D005615:Freezing; D005947:Glucose / Q000494:pharmacology; D008297:Male; D008744:Methylamines / Q000494:pharmacology; D013048:Specimen Handling / Q000662:veterinary*; D013094:Spermatozoa / Q000187:drug effects*; D014199:Trehalose / Q000494:pharmacology*
D000179:Acrylates; D003451:Cryoprotective Agents; D008744:Methylamines; C053576:N-acryloyl-tris(hydroxymethyl)aminomethane; D019343:Citric Acid; D014199:Trehalose; D005947:Glucose