[Research study on renal targeted chemotherapy with magnetic harpoons and intravenous administration of ferro-carbon nanoparticles].
OBJECTIVE
The use of nanoparticules for drug transport is one of the topics with priority interest within the field of biomedical research. Our objective is to show the initial results of an innovative method to focalize drug carrier ferro-carbon nanoparticules to solid organs. We obtained and characterized various types of ferrous magnetic nanoparticules and studied their behaviour in vitro and in vivo in laboratory animals with intrarenal magnetic targets laparoscopically implanted.


METHODS
Using a plasma arch we obtained ferro-carbon nanoparticules with the ability to absorb and deliver doxorubicin, showing an excellent behaviour in in vitro rheological studies. Under general anesthesia and control we inserted a gold covered magnetic microharpoon in the left kidney of New Zealand rabbits. At the same time we injected intravenously different doses of various types of nanoparticules. The animals were sacrified ofter pre-established times and pathologic studies of their kidneys, spleens, livers, lungs and bone marrow were carried out.


RESULTS
After selection of the most adequafe nanoparticules for our purposes, we ascertained significant differences in the distribution of nanoparticules in postmortem studies, with accumulation in the magnetic target and surrounding renal parenchyma. Nevertheless, the reticuloendothelial system retains a great amount of the injected dose.


CONCLUSIONS
Although our magnetic focalization system is effective, nanoparticule temporary protection systems should be tested to allow us avoid the action of the immune system.
Valdivia Uríac
José Gabriel
JG
Instituto Universitario de Investigación en Nanociencia de Aragón, Universidad de Zaragoza, Zaragoza. valdivia@unizar.es
Ibarra García
Manuel Ricardo
MR
Fernández Pacheco
Rodrigo
R
Viloria
Américo
A
Higuera
Teresa
T
Laborda
Alicia
A
García
Angel
A
García de Jalón
José Antonio
JA
Gutiérrez
Martín
M
Romero
Maria Soledad
MS
Cornudella
Rosa
R
Arruebo
Manuel
M
Marquina
Clara
C
Arbiol
Jordi
J
Santamaría
Jesús
J
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage*; D004317:Doxorubicin / Q000008:administration & dosage*; D004337:Drug Carriers; D006801:Humans; D007275:Injections, Intravenous; D007680:Kidney Neoplasms / Q000188:drug therapy*; D053758:Nanoparticles / Q000008:administration & dosage*; D011817:Rabbits
D000903:Antibiotics, Antineoplastic; D004337:Drug Carriers; D004317:Doxorubicin