title: Differential cerebral cortex transcriptomes of baboon neonates consuming moderate and high docosahexaenoic acid formulas.
abstract: BACKGROUND
  Docosahexaenoic acid (DHA, 22:6n-3) and arachidonic acid (ARA, 20:4n-6) are the major long chain polyunsaturated fatty acids (LCPUFA) of the central nervous system (CNS). These nutrients are present in most infant formulas at modest levels, intended to support visual and neural development. There are no investigations in primates of the biological consequences of dietary DHA at levels above those present in formulas but within normal breastmilk levels.
  
  
  RESULTS
  Twelve baboons were divided into three formula groups: Control, with no DHA-ARA; "L", LCPUFA, with 0.33%DHA-0.67%ARA; "L3", LCPUFA, with 1.00%DHA-0.67%ARA. All the samples are from the precentral gyrus of cerebral cortex brain regions. At 12 weeks of age, changes in gene expression were detected in 1,108 of 54,000 probe sets (2.05%), with most showing <2-fold change. Gene ontology analysis assigns them to diverse biological functions, notably lipid metabolism and transport, G-protein and signal transduction, development, visual perception, cytoskeleton, peptidases, stress response, transcription regulation, and 400 transcripts having no defined function. PLA2G6, a phospholipase recently associated with infantile neuroaxonal dystrophy, was downregulated in both LCPUFA groups. ELOVL5, a PUFA elongase, was the only LCPUFA biosynthetic enzyme that was differentially expressed. Mitochondrial fatty acid carrier, CPT2, was among several genes associated with mitochondrial fatty acid oxidation to be downregulated by high DHA, while the mitochondrial proton carrier, UCP2, was upregulated. TIMM8A, also known as deafness/dystonia peptide 1, was among several differentially expressed neural development genes. LUM and TIMP3, associated with corneal structure and age-related macular degeneration, respectively, were among visual perception genes influenced by LCPUFA. TIA1, a silencer of COX2 gene translation, is upregulated by high DHA. Ingenuity pathway analysis identified a highly significant nervous system network, with epidermal growth factor receptor (EGFR) as the outstanding interaction partner.
  
  
  CONCLUSIONS
  These data indicate that LCPUFA concentrations within the normal range of human breastmilk induce global changes in gene expression across a wide array of processes, in addition to changes in visual and neural function normally associated with formula LCPUFA.
authors.affiliation: Division of Nutritional Sciences, Cornell University, Savage Hall, Ithaca, New York, United States of America.
authors.forename: Kumar S D
authors.initials: KS
authors.lastname: Kothapalli
authors.forename: Joshua C
authors.initials: JC
authors.lastname: Anthony
authors.forename: Bruce S
authors.initials: BS
authors.lastname: Pan
authors.forename: Andrea T
authors.initials: AT
authors.lastname: Hsieh
authors.forename: Peter W
authors.initials: PW
authors.lastname: Nathanielsz
authors.forename: J Thomas
authors.initials: JT
authors.lastname: Brenna
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis; D002448:Cell Adhesion; D002540:Cerebral Cortex / Q000378:metabolism*; D004281:Docosahexaenoic Acids / Q000008:administration & dosage*; D019204:GTP-Binding Proteins / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D010215:Papio / Q000235:genetics*; D015398:Signal Transduction; D014796:Visual Perception
chemical_list: D004281:Docosahexaenoic Acids; D019204:GTP-Binding Proteins