Tumour necrosis factor-alpha (TNFalpha) and interleukin-10 are crucial mediators in post-operative systemic inflammatory response and determine the occurrence of complications after major abdominal surgery.
BACKGROUND
The course of serum cytokine levels in patients with postoperative systemic inflammatory response syndrome (SIRS) after major abdominal surgery remains currently unclear.


METHODS
Blood was sampled pre- and post-operatively and on days 1 and 2 in 40 patients undergoing major abdominal surgery. Concentrations of tumour necrosis factor-alpha (TNFalpha), interleukin (IL) -6, IL-8, and IL-10 were measured by the LINCOplex assay; those of soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) by an enzyme immunoassay.


RESULTS
Compared to their pre-operative values, sTREM-1 was elevated on day 2; TNFalpha on day 1; IL-6 and IL-10 post-operatively and on days 1 and 2; and IL-8 post-operatively and on day 1. The duration of operation correlated with TNFalpha and IL-10 at all sampling times, and with IL-6 post-operatively. There were no differences in cytokine concentrations between patients who exhibited post-operative complications and those who did not. IL-10/TNFalpha below 30 was found in all patients with complications (100%) and in 20 patients without complications (64.5%, p: 0.043).


CONCLUSIONS
SIRS following major surgery is characterised by complex alterations in cytokine concentrations. The balance between TNFalpha and IL-10 seems to determine the occurrence of post-operative complications.
Dimopoulou
Ioanna
I
2nd Department of Critical Care Medicine, ATTIKON Hospital, University of Athens, Medical School, Greece.
Armaganidis
Apostolos
A
Douka
Evangelia
E
Mavrou
Irini
I
Augustatou
Callirhoe
C
Kopterides
Petros
P
Lyberopoulos
Panagiotis
P
Tzanela
Marinella
M
Orfanos
Stylianos E
SE
Pelekanou
Emilia
E
Kostopanagiotou
Georgia
G
Macheras
Anastasios
A
Giamarellos-Bourboulis
Evangelos J
EJ
D000005:Abdomen / Q000601:surgery*; D000368:Aged; D015415:Biomarkers; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000097:blood*; D015850:Interleukin-6 / Q000097:blood; D016209:Interleukin-8 / Q000097:blood; D008297:Male; D008562:Membrane Glycoproteins / Q000097:blood; D011183:Postoperative Complications / Q000097:blood* / Q000473:pathology; D011971:Receptors, Immunologic / Q000097:blood; D018746:Systemic Inflammatory Response Syndrome / Q000097:blood* / Q000209:etiology* / Q000473:pathology; D000073871:Triggering Receptor Expressed on Myeloid Cells-1; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*
D015415:Biomarkers; D015850:Interleukin-6; D016209:Interleukin-8; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic; C408842:TREM1 protein, human; D000073871:Triggering Receptor Expressed on Myeloid Cells-1; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10