[Role of malnutrition in intestinal anastomosis collagenization: an analysis of procollagen (PINP) and carboxyterminal telopeptide (ICTP) by radioimmunoassay].
BACKGROUND
some clinical, anatomo-pathological, and technical factors influence the correct healing of intestinal suture following an intestinal resection. One of the most influential factors is patient nutritional status.


OBJECTIVE
to evaluate the influence of malnutrition on the viability of primary intestinal anastomosis by the analysis of collagen I deposition.


METHODS
40 Wistar rats, radioimmunoassay material. We used 2 groups of rats, 20 animals in each group: a control group (A) and a "malnutrition" group (B).


RESULTS
there was a decrease in PINP (procollagen) deposition in the colon of group B rats as compared to the colon of group A (0.3620 and 0.4340 mg/g respectively) (p = 0.032). There is an increase in ICTP (carboxyterminal telopeptide) in the colon of group B (0.9545 as against 0.8460 mg/g in group A) (p = 0.875). In anastomoses of group B there was a decrease in PINP synthesis as compared to group A (0.376 and 0.468 mg/g respectively, p = 0.002). As regards ICTP, there was an increase in group B (p = 0.330). In relation to the control group no differences were observed in ICTP increases in group B (p = 1).


CONCLUSIONS
colonic anastomosis increases the levels of PINP and ICTP in healed tissue (p = 0.000); malnutrition reduces collagenization in anastomoses (p = 0.000).
Alamo
J M
JM
Servicio de Cirugía General y de Aparato Digestivo, Hospitales Universitarios Virgen del Rocío, Sevilla.
Galindo
A
A
Morales
S
S
Daza
G
G
Socas
M
M
Suárez-Artacho
G
G
Suárez-Grau
J M
JM
García-Moreno
J
J
Pareja
F
F
Gómez
M A
MA
D000714:Anastomosis, Surgical; D000818:Animals; D024042:Collagen Type I; D003106:Colon / Q000601:surgery*; D004195:Disease Models, Animal; D005260:Female; D044342:Malnutrition / Q000150:complications*; D009752:Nutritional Status; D010446:Peptide Fragments / Q000032:analysis*; D010455:Peptides; D011347:Procollagen / Q000032:analysis*; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing
D024042:Collagen Type I; D010446:Peptide Fragments; D010455:Peptides; D011347:Procollagen; C052929:collagen type I trimeric cross-linked peptide; C042813:procollagen type I carboxy terminal peptide