Comparison of the phenotyping methods ID 32E and VITEK 2 compact GN with 16S rRNA gene sequencing for the identification of Enterobacter sakazakii.
A total of 34 isolates (28 Enterobacter sakazakii and 6 Enterobacteriaceae) from infant formulae, milk powder, and the production environment of milk powder factories were identified using ID 32E and VITEK 2 compact GN systems (bioMérieux, France). The ID 32E version 3.0 and VITEK 2 compact GN version 01.01b correctly identified 100% (28) of the Enterobacter sakazakii isolates tested, whereas the previous software version 2.0 for ID 32E showed only 71.4% correct results. None of the non-E. sakazakii isolates tested were misidentified as E. sakazakii with either of the identification systems used.
Fanjat
Nadège
N
bioMerieux, Research and Development Microbiology, 38 390 La Balme Les Grottes, France.
Leclercq
Alexandre
A
Joosten
Han
H
Robichon
Denis
D
D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods*; D044083:Cronobacter sakazakii / Q000145:classification* / Q000235:genetics / Q000502:physiology; D005506:Food Contamination; D005516:Food Microbiology; D020459:Genes, rRNA; D006801:Humans; D041943:Infant Formula; D008969:Molecular Sequence Data; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011208:Powders; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D011933:Reagent Kits, Diagnostic; D017422:Sequence Analysis, DNA; D012984:Software
D011208:Powders; D012336:RNA, Ribosomal, 16S; D011933:Reagent Kits, Diagnostic