Light and metabolic signals control the selective degradation of sucrose synthase in maize leaves during deetiolation.
The content and activity of Suc (Suc) synthase (SUS) protein is high in sink organs but low in source organs. In this report, we examined light and metabolic signals regulating SUS protein degradation in maize (Zea mays) leaves during deetiolation. We found that SUS protein accumulated in etiolated leaves of the dark-grown seedlings but was rapidly degraded upon exposure to white, blue, or red light. This occurred concurrent with the accumulation of photosynthetic enzymes, such as Rubisco and Rubisco activase, and enzymes of Suc biosynthesis such as Suc-phosphate synthase. Deetiolation-induced SUS degradation was not inhibited by the proteasome inhibitor MG132. Moreover, neither full-length nor truncated SUS phosphorylated at the serine-170 site was found in the crude 26S proteasome fraction (150,000g postmicrosomal pellet) isolated in the presence of MG132. However, SUS degradation was strongly inhibited by feeding cycloheximide or amino acids to detached leaves, while Suc feeding had no effect. Of the amino acids tested, exogenous glutamate had the greatest effect. Collectively, these results demonstrate that SUS protein degradation during deetiolation: (1) is selective; (2) can be triggered by either blue- or red light-mediated signaling pathways; (3) does not involve the 26S proteasome; and (4) is inhibited by free amino acids. These findings suggest that SUS degradation is important to supply residues for the synthesis of other proteins required for autotrophic metabolism.
Qiu
Quan-Sheng
QS
Department of Plant Biology, University of Illinois, Urbana, IL 61801, USA.
Hardin
Shane C
SC
Mace
Jacob
J
Brutnell
Thomas P
TP
Huber
Steven C
SC
D000596:Amino Acids / Q000494:pharmacology; D005964:Glucosyltransferases / Q000378:metabolism*; D008027:Light; D018515:Plant Leaves / Q000254:growth & development / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism; D036226:Seedlings / Q000254:growth & development / Q000378:metabolism; D015398:Signal Transduction; D013395:Sucrose / Q000494:pharmacology; D003313:Zea mays / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D017871:Calcium-Calmodulin-Dependent Protein Kinases
D000596:Amino Acids; D005964:Glucosyltransferases; D010940:Plant Proteins; D046988:Proteasome Endopeptidase Complex; D011494:Protein Kinases; D013395:Sucrose; D017871:Calcium-Calmodulin-Dependent Protein Kinases; C022699:sucrose synthase; C061553:ATP dependent 26S protease