A simple method to determine concentration of enantiomers in enzyme-catalyzed kinetic resolution.
Kinetic resolutions play important roles in industrial biotransformations for production of optical pure compounds from racemic substrates. A simple method, based on enantiomeric excess of both substrate (ee (S)) and the corresponding product (ee (P)), was developed for determination of concentration of enantiomers in kinetic resolution. Since only relative quantity (ee) was required in the proposed method, calibration and cumbersome quantitative sample handling can be avoided and analytical accuracy can be greatly improved.
Zheng
Ren-Chao
RC
Institute of Bioengineering, Zhejiang University of Technology, Hangzhou, PR China.
Zheng
Yu-Guo
YG
Shen
Yin-Chu
YC
D000581:Amidohydrolases / Q000378:metabolism*; D002384:Catalysis; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D007700:Kinetics; D013237:Stereoisomerism; D013379:Substrate Specificity
D000581:Amidohydrolases; C019665:amidase