Seed and agronomic QTL in low linolenic acid, lipoxygenase-free soybean (Glycine max (L.) Merrill) germplasm.
Linolenic acid and seed lipoxygenases are associated with off flavours in soybean products. F5 recombinant inbred lines (RILs) from a cross between a low linolenic acid line (RG10) and a seed lipoxygenase-free line (OX948) were genotyped for simple sequence repeats (SSR), random amplified polymorphic DNA (RAPD), sequence-tagged sites (STS), and cleaved amplified polymorphic sequence (CAPS) markers and evaluated for seed and agronomic traits at 3 Ontario locations in 2 years. One hundred twenty markers covering 1247.5 cM were mapped to 18 linkage groups (LGs) in the soybean composite genetic map. Seed lipoxygenases L-1 and L-2 mapped as single major genes to the same location on LG G13-F. L-3 mapped to LG G11-E. This is the first report of a map position for L-3. A major quantitative trait locus (QTL) associated with reduced linolenic acid content was identified on LG G3-B2. QTLs for 12 additional seed and agronomic traits were detected. Linolenic acid content, linoleic acid content, yield, seed mass, protein content, and plant height QTL were present in at least 4 of 6 environments. Three to 8 QTLs per trait were detected that accounted for up to 78% of total variation. Linolenic acid and lipoxygenase loci did not overlap yield QTL, suggesting that it should be possible to develop high-yielding lines resistant to oxidative degradation by marker-assisted selection (MAS).
Reinprecht
Yarmilla
Y
Department of Plant Agriculture, University of Guelph, Guelph, ON N1G 2W1, Canada.
Poysa
Vaino W
VW
Yu
Kangfu
K
Rajcan
Istvan
I
Ablett
Gary R
GR
Pauls
K Peter
KP
D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D003433:Crosses, Genetic; D005227:Fatty Acids / Q000235:genetics; D035263:Flowering Tops / Q000235:genetics; D008084:Lipoxygenase / Q000235:genetics*; D018598:Minisatellite Repeats; D010938:Plant Oils / Q000032:analysis; D030821:Plants, Genetically Modified; D040641:Quantitative Trait Loci; D012639:Seeds / Q000235:genetics* / Q000254:growth & development; D013025:Glycine max / Q000235:genetics* / Q000254:growth & development; D017962:alpha-Linolenic Acid / Q000032:analysis* / Q000235:genetics
D005227:Fatty Acids; D010938:Plant Oils; D017962:alpha-Linolenic Acid; D008084:Lipoxygenase