Penicillin-bound polyacrylate nanoparticles: restoring the activity of beta-lactam antibiotics against MRSA.
This report describes the preparation of antibacterially active emulsified polyacrylate nanoparticles in which a penicillin antibiotic is covalently conjugated onto the polymeric framework. These nanoparticles were prepared in water by emulsion polymerization of an acrylated penicillin analogue pre-dissolved in a 7:3 (w:w) mixture of butyl acrylate and styrene in the presence of sodium dodecyl sulfate (surfactant) and potassium persulfate (radical initiator). Dynamic light scattering analysis and atomic force microscopy images show that the emulsions contain nanoparticles of approximately 40 nm in diameter. The nanoparticles have equipotent in vitro antibacterial properties against methicillin-susceptible and methicillin-resistant forms of Staphylococcus aureus and indefinite stability toward beta-lactamase.
Turos
Edward
E
Center for Molecular Diversity in Drug Design, Discovery, and Delivery, Department of Chemistry, 4202 East Fowler Avenue, CHE 207, University of South Florida, Tampa, FL 33620, USA. eturos@shell.cas.usf.edu
Reddy
G Suresh Kumar
GS
Greenhalgh
Kerriann
K
Ramaraju
Praveen
P
Abeylath
Sampath C
SC
Jang
Seyoung
S
Dickey
Sonja
S
Lim
Daniel V
DV
D000180:Acrylic Resins / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry; D008712:Methicillin / Q000494:pharmacology; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests; D018625:Microscopy, Atomic Force; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010406:Penicillins / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D012542:Scattering, Radiation; D013211:Staphylococcus aureus / Q000187:drug effects*; D047090:beta-Lactams / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*
D000180:Acrylic Resins; D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D010406:Penicillins; D047090:beta-Lactams; D008712:Methicillin