title: Regulation of MMP-1 by sex steroid hormones in fibroblasts derived from the female pelvic floor.
abstract: OBJECTIVE
  The aim of this study was to investigate the effect of sex steroid hormones on the overall expression profile of cellular matrix metalloproteinase-1 (MMP-1) in fibroblasts that are derived from arcus tendineus fasciae pelvis.
  
  
  METHODS
  Arcus tendineus fasciae pelvis fibroblasts that originated from a premenopausal woman and a postmenopausal woman who was undergoing a prolapse repair were treated with physiologic concentrations of 17-beta-estradiol (E2), progesterone, E2 plus progesterone, and E2 plus ICI 182,780. Cellular expressions of the latent, active, and fragment forms of MMP-1 were analyzed quantitatively by Western immunoblotting.
  
  
  RESULTS
  The latent and fragment forms of MMP-1 were increased by E2, progesterone, and E2 plus progesterone. The active form of MMP-1 was not changed by either E2 or progesterone alone but was decreased significantly when both hormones were added together. ICI 182,780 inhibited the stimulatory effect of E2.
  
  
  CONCLUSIONS
  Fragmentation is a site of regulation of MMP-1 expression by hormones. Only E2 combined with progesterone decreased the active form of MMP-1, which suggests that both hormones are necessary to maintain the integrity of female pelvic floor.
authors.affiliation: Division of Urogynecology and Reconstructive Pelvic Surgery, Department of Obstetrics and Gynecology and Reproductive Sciences, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Zong
authors.forename: Halina M
authors.initials: HM
authors.lastname: Zyczynski
authors.forename: Leslie A
authors.initials: LA
authors.lastname: Meyn
authors.forename: Susan C
authors.initials: SC
authors.lastname: Gordy
authors.forename: Pamela A
authors.initials: PA
authors.lastname: Moalli
mesh_terms: D000328:Adult; D000368:Aged; D015153:Blotting, Western; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology*; D005455:Fluorescent Antibody Technique; D012739:Gonadal Steroid Hormones / Q000494:pharmacology*; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000187:drug effects* / Q000378:metabolism*; D008875:Middle Aged; D017773:Pelvic Floor; D017698:Postmenopause; D017697:Premenopause; D011336:Probability; D012494:Sampling Studies; D012680:Sensitivity and Specificity
chemical_list: D012739:Gonadal Steroid Hormones; D020781:Matrix Metalloproteinase 1