Determination of catecholamines and indoleamines in human urine based on intramolecular excimer-forming derivatization and fluorescence detection.
A liquid chromatographic (LC) determination of catecholamines and indoleamines is described. This is based on intramolecular excimer-forming fluorescence derivatization with 4-(1-pyrene)butanoyl chloride, followed by reversed-phase LC. The analytes, containing an amino moiety and phenolic hydroxyl moieties in a molecule, were converted to the corresponding polypyrene-labeled derivatives by one-step derivatization. They afforded intramolecular excimer fluorescence, which can clearly be discriminated from the normal fluorescence emitted from reagent blanks. The detection limits (S/N = 3) for catecholamines and indoleamines were femto-mole levels per 20-microL injection. Furthermore, this method was applied to a urine assay.
Yoshida
Hideyuki
H
Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, Japan.
Kido
Fumito
F
Yoshitake
Makoto
M
Todoroki
Kenichiro
K
Nohta
Hitoshi
H
Yamaguchi
Masatoshi
M
D006916:5-Hydroxytryptophan / Q000652:urine*; D001679:Biogenic Amines / Q000652:urine*; D002073:Butanes / Q000737:chemistry*; D002395:Catecholamines / Q000652:urine*; D002853:Chromatography, Liquid / Q000379:methods; D005453:Fluorescence; D006801:Humans; D011721:Pyrenes / Q000737:chemistry*; D012701:Serotonin / Q000652:urine
C499821:4-(1-pyrene)butanoyl chloride; D001679:Biogenic Amines; D002073:Butanes; D002395:Catecholamines; D011721:Pyrenes; D012701:Serotonin; D006916:5-Hydroxytryptophan