Inhibition of severe acute respiratory syndrome-associated coronavirus infection by equine neutralizing antibody in golden Syrian hamsters.
Equine anti-severe acute respiratory syndrome-associated coronavirus F(ab')(2) has been verified to protect mice from infection with severe acute respiratory syndrome-associated coronavirus (SARS-CoV). However, before potential clinical application, the antibody needs to be tested in as many animal models as possible to ensure its safety and efficiency. In this study, after verification by various methods that the golden Syrian hamster constitutes a model susceptible to SARS-CoV infection, we confirmed that the antibody could protect animals completely from SARS-CoV infection in the preventive setting. More importantly, the antibody could reduce viral titers or copies by approximately 10(3)- to 10(4)-fold in animal lung after virus exposure, compared with negative control. These data provide further evidence to warrant clinical studies of this antibody in the treatment and prevention of SARS.
Zhao
Guangyu
G
Department of Immunology, Institute of Microbiology Epidemiology, Academy of Military Medical Sciences, Beijing, People's Republic of China.
Ni
Bing
B
Jiang
Haiyan
H
Luo
Deyan
D
Pacal
Marek
M
Zhou
Lili
L
Zhang
Liangyan
L
Xing
Li
L
Zhang
Liangyan
L
Jia
Zhengcai
Z
Lin
Zhihua
Z
Wang
Li
L
Li
Jintao
J
Liang
Yunfei
Y
Shi
Xinfu
X
Zhao
Tingting
T
Zhou
Liyun
L
Wu
Yuzhang
Y
Wang
Xiliang
X
D000281:Administration, Intranasal; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D006224:Cricetinae; D006736:Horses; D007140:Immunoglobulin Fab Fragments / Q000276:immunology*; D008647:Mesocricetus; D009500:Neutralization Tests; D016133:Polymerase Chain Reaction; D045169:Severe Acute Respiratory Syndrome / Q000517:prevention & control*
D000914:Antibodies, Viral; D007140:Immunoglobulin Fab Fragments