[Spleen tyrosine kinase (L) suppresses breast cancer development by downregulating the expression of cyclin D1, ID1, B-myb and Fra1].
OBJECTIVE
To screen the target genes regulated by the transcriptional repressor Syk (L) so as to explore the mechanism of Syk (L) in suppressing breast cancer.


METHODS
Adeno-X expression system from Clontech Company was used to construct the adenovirus-Syk. Syk negative breast cancer cells of the line MB231 were cultured and infected with adeno-Syk (L), adeno-Syk (S), and the control adeno-Lac Z respectively. cDNA microarray was used to screen the genes regulated by Syk (L) and Syk (S). Northern blotting was used to examine the cDNA microarray results.


RESULTS
The transcriptional repressor Syk (L) was able to down-regulate the expression of the oncogenes ID1, cyclin D1, Fra1, and B-myb in the breast cancer cells. Northern blotting confirmed this regulation.


CONCLUSIONS
Syk (L) suppresses the progression of breast cancer by down regulating the expression of the oncogenes ID1, cyclin D1, Fra1, and B-myb.
Wang
Lei
L
Department of General Surgery, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
Wang
Jian-Ping
JP
Song
Xin-Ming
XM
He
Yu-Long
YL
D000256:Adenoviridae / Q000235:genetics; D015152:Blotting, Northern; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology; D018797:Cell Cycle Proteins / Q000235:genetics; D045744:Cell Line, Tumor; D019938:Cyclin D1 / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D051798:Inhibitor of Differentiation Protein 1 / Q000235:genetics; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics; D000072377:Syk Kinase; D015534:Trans-Activators / Q000235:genetics; D014162:Transfection; D000099169:Fos-Related Antigen 1
D018797:Cell Cycle Proteins; D019938:Cyclin D1; D051798:Inhibitor of Differentiation Protein 1; D047908:Intracellular Signaling Peptides and Proteins; D011505:Protein-Tyrosine Kinases; D016760:Proto-Oncogene Proteins c-fos; D000072377:Syk Kinase; D015534:Trans-Activators; D000099169:Fos-Related Antigen 1; C497453:ID1 protein, human; C064352:MYBL2 protein, human; C000606896:SYK protein, human