Studying the uptake of cell-penetrating peptides.
More than a decade ago, it was discovered that cationic peptides could traverse the cellular plasma membrane without specific transporter proteins or membrane damage. Subsequently, it was found that these peptides, known as cell-penetrating peptides (CPPs), were also capable of delivering cargos into cells, hence the great potential of these vectors was acknowledged. Today, many different research groups are working with CPPs, which necessitates efforts to develop unified assays enabling the comparison of data. Here we contribute three protocols for evaluation of CPPs which, if used in conjunction, provide complementary data about the amount and mechanism of uptake (fluorometric analysis and confocal microscopy, respectively), as well as the extent of degradation (HPLC analysis of cell lysates). All three protocols are based on the use of fluorescently labeled peptides and can be performed on the same workday.
Holm
Tina
T
Department of Neurochemistry, Stockholm University, S. Arrheniusv. 21A, SE-106 91, Stockholm, Sweden.
Johansson
Henrik
H
Lundberg
Pontus
P
Pooga
Margus
M
Lindgren
Maria
M
Langel
Ulo
U
D002412:Cations / Q000737:chemistry; D002462:Cell Membrane / Q000378:metabolism*; D002463:Cell Membrane Permeability; D005452:Fluoresceins / Q000737:chemistry / Q000378:metabolism; D005470:Fluorometry / Q000379:methods*; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism*; D021381:Protein Transport
D002412:Cations; D005452:Fluoresceins; D010455:Peptides