Double-inducible gene activation system for caspase 3 and 9 in epidermis.
Expression of genes with tight and precise temporal and spatial control is desired in a wide variety of applications ranging from cultured cells and transgenic animals to gene therapy. While current inducible systems, such as RU486 and chemical inducers of dimerization (CID), have improved earlier inducible models (Gossen et al., 1995, Science. 268:1766-1769; Wang et al., 1994, Proc Natl Acad Sci USA 91:8180-8184), no single system is perfect at present. One potential drawback of these systems is leakage of transgene expression, causing limitations of each system. We have developed an inducible model containing both RU486 and CID systems, which in addition to inducing caspase activation, has potential applicability specifically to other genes encoding proteins that require a dimerization event for activation. This Double-Inducible Gene Activation System generates two barriers for the target gene expression and protein activation thereby minimizing leakage.
Shah
Viraj R
VR
Center for Molecular Development and Disease, Texas A and M University Health Science Center, Institute of Biosciences and Technology, Houston, Texas 77030, USA.
Koster
Maranke I
MI
Roop
Dennis R
DR
Spencer
David M
DM
Wei
Lei
L
Li
Qi
Q
Schwartz
Robert J
RJ
Chang
Jiang
J
D000818:Animals; D000831:Animals, Newborn; D053148:Caspase 3 / Q000235:genetics* / Q000378:metabolism; D053453:Caspase 9 / Q000235:genetics* / Q000378:metabolism; D003433:Crosses, Genetic; D004817:Epidermis / Q000378:metabolism*; D005786:Gene Expression Regulation; D005818:Genetic Engineering / Q000379:methods*; D007150:Immunohistochemistry; D051379:Mice; D008822:Mice, Transgenic; D015735:Mifepristone / Q000494:pharmacology; D008954:Models, Biological; D009928:Organ Specificity; D011485:Protein Binding / Q000187:drug effects; D015533:Transcriptional Activation
D015735:Mifepristone; C505409:Casp3 protein, mouse; C506434:Casp9 protein, mouse; D053148:Caspase 3; D053453:Caspase 9