Presynaptic GABA-A receptors prevent depression of nicotinic transmission in rabbit coeliac ganglion neurones.
We investigated the involvement of GABA-A receptors in the modulation of the nicotinic transmission of central origin in isolated rabbit coeliac ganglia. Our study was performed in vitro and the electrical activity of the ganglionic neurones was recorded using intracellular recording techniques. During iterative stimulation of the splanchnic nerves, the synaptic action potential probability decreased gradually, indicating a depression of the nicotinic activation. Pharmacological agents acting at GABA-A receptors modulated the action potential probability during the train of pulses. Muscimol (a GABA-A receptor agonist), diazepam (a benzodiazepine site agonist) and 1-[2-[[(diphenylmethylene)imino]oxy]ethyl]-1,2,5,6-tetrahydro-3-pyridinecarboxylic acid hydrochloride (a GABA uptake blocker) increased this probability. Conversely, gabazine or bicuculline (two GABA-A receptor antagonists), picrotoxin (a picrotoxin site agonist) and flumazenil (a benzodiazepine site antagonist) reduced it. These results demonstrate that endogenous GABA, released during the train of pulses, facilitates the central nicotinic activation of the ganglionic neurones by acting on GABA-A receptors. Muscimol also reduced the amplitude ratio of excitatory postsynaptic potentials triggered during the paired-pulse protocol without any change in postsynaptic properties. This result is consistent with a presynaptic action of GABA-A receptors. Our study shows that presynaptic GABA-A receptors facilitate the central nicotinic activation of prevertebral ganglionic neurones and thus play a novel role in the integrative properties of the sympathetic prevertebral ganglia.
Ercoli
Jennifer
J
Laboratoire de Physiologie Neurovégétative, UMR Université Paul Cézanne Aix-Marseille III-CNRS-INRA, Faculté des Sciences et Techniques, 13397 Marseille cedex 20, France.
Miolan
Jean Pierre
JP
Niel
Jean Pierre
JP
Quinson
Nathalie
N
D000200:Action Potentials / Q000187:drug effects / Q000528:radiation effects; D000818:Animals; D004347:Drug Interactions; D005260:Female; D018682:GABA Agents / Q000494:pharmacology; D005728:Ganglia, Sympathetic / Q000166:cytology*; D005731:Ganglionic Stimulants / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009538:Nicotine / Q000494:pharmacology*; D011817:Rabbits; D011930:Reaction Time / Q000187:drug effects / Q000502:physiology; D011963:Receptors, GABA-A / Q000502:physiology*; D013153:Splanchnic Nerves / Q000502:physiology / Q000528:radiation effects; D009435:Synaptic Transmission / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects
D018682:GABA Agents; D005731:Ganglionic Stimulants; D011963:Receptors, GABA-A; D009538:Nicotine