title: Expression and protective role of heme oxygenase-1 in delayed myocardial preconditioning.
abstract: In the study the authors aimed to demonstrate the expression and protective effect of heme oxygenase-1 (HO-1) in the delayed preconditioning (PC) on cultured myocardiac cells. Neonatal rat cardiac myocytes were exposed to ischemic (ischemic medium [IM] for 20 min) and pharmacological (adenosine, epinephrine, opioid) PC. Twenty-four hours later cells were subjected to a simulated ischemia (SI)--culturing for 3 h in IM, followed by 2-h reperfusion in normal medium--and then lactate dehydrogenase (LDH), live/death ratio, and apoptosis were measured. For demonstrating the protective role of HO-1, its enzymatic activity was competitively inhibited by administration of zinc protoporphyrin IX (ZnPPIX), and HO-1 synthesis was blocked with HO-1 siRNA. Cells in control group were cultured under normoxic conditions. In SI group, cells underwent only an SI without PC. HO-1 expression in all of the groups was demonstrated with immunostaining. Our results showed a significant decrease of LDH release, apoptosis, and cell death in PC groups versus SI group, which has been risen in ZnPPIX- and HO-1 siRNA-treated groups. HO-1 immunostaining showed an appreciable HO-1 expression in PC groups, which was abolished with HO-1 siRNA administration, but not in ZnPPIX group. The results therefore suggest that HO-1 expression increases in both ischemic and pharmacological PC, and HO-1 has cellular protective effect against cell death and apoptosis in ischemia-reperfusion-induced oxidative injury.
authors.affiliation: Department of Surgical Research and Techniques, University of Pécs, Medical Faculty, Kodaly Zoltan St. 20, Pécs, H-7624 Hungary. jancsogabor@hotmail.com
authors.forename: Gábor
authors.initials: G
authors.lastname: Jancsó
authors.forename: Barbara
authors.initials: B
authors.lastname: Cserepes
authors.forename: Balázs
authors.initials: B
authors.lastname: Gasz
authors.forename: László
authors.initials: L
authors.lastname: Benkó
authors.forename: Balázs
authors.initials: B
authors.lastname: Borsiczky
authors.forename: Andrea
authors.initials: A
authors.lastname: Ferenc
authors.forename: Mária
authors.initials: M
authors.lastname: Kürthy
authors.forename: Boglárka
authors.initials: B
authors.lastname: Rácz
authors.forename: János
authors.initials: J
authors.lastname: Lantos
authors.forename: János
authors.initials: J
authors.lastname: Gál
authors.forename: Endre
authors.initials: E
authors.lastname: Arató
authors.forename: Lószló
authors.initials: L
authors.lastname: Sínayc
authors.forename: György
authors.initials: G
authors.lastname: Wéber
authors.forename: Erzsébet
authors.initials: E
authors.lastname: Róth
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D051547:Heme Oxygenase-1 / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D019157:Ischemic Preconditioning, Myocardial; D009206:Myocardium / Q000166:cytology / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D051547:Heme Oxygenase-1