title: Identification of the novel narrow-spectrum beta-lactamase SCO-1 in Acinetobacter spp. from Argentina.
abstract: By studying the beta-lactamase content of several Acinetobacter spp. isolates from Argentina, producing the expanded-spectrum beta-lactamases (ESBL) VEB-1a or PER-2, a novel Ambler class A beta-lactamase gene was identified. It encoded the narrow-spectrum beta-lactamase SCO-1, whose activity was inhibited by clavulanic acid. SCO-1 hydrolyzes penicillins at a high level and cephalosporins and carbapenems at a very low level. beta-Lactamase SCO-1 was identified from unrelated VEB-1a-positive or PER-2-positive Acinetobacter spp. isolates recovered from three hospitals. The bla(SCO-1) gene was apparently located on a plasmid of ca. 150 kb from all cases but was not associated with any ESBL-encoding gene. The G+C content of the bla(SCO) gene was 52%, a value that does not correspond to that of the A. baumannii genome (39%). beta-Lactamase SCO-1 shares 47% amino acid identity with CARB-5 and ca. 40% with the enzymes TEM, SHV, and CTX-M. A gene encoding a putative resolvase was identified downstream of the bla(SCO-1) gene, but its precise way of acquisition remains to be determined.
authors.affiliation: Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre cedex, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Poirel
authors.forename: Stéphane
authors.initials: S
authors.lastname: Corvec
authors.forename: Melina
authors.initials: M
authors.lastname: Rapoport
authors.forename: Pauline
authors.initials: P
authors.lastname: Mugnier
authors.forename: Alejandro
authors.initials: A
authors.lastname: Petroni
authors.forename: Fernando
authors.initials: F
authors.lastname: Pasteran
authors.forename: Diego
authors.initials: D
authors.lastname: Faccone
authors.forename: Marcelo
authors.initials: M
authors.lastname: Galas
authors.forename: Henri
authors.initials: H
authors.lastname: Drugeon
authors.forename: Vincent
authors.initials: V
authors.lastname: Cattoir
authors.forename: Patrice
authors.initials: P
authors.lastname: Nordmann
mesh_terms: D000150:Acinetobacter / Q000145:classification / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D000151:Acinetobacter Infections / Q000382:microbiology; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001118:Argentina; D019818:Clavulanic Acid / Q000378:metabolism / Q000494:pharmacology; D003001:Cloning, Molecular; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D047090:beta-Lactams / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D019818:Clavulanic Acid; D001618:beta-Lactamases