Intraocular biodistribution of mono-L-aspartyl chlorin e6 in a primate choroidal neovascularization model.
To describe the biodistribution of a hydrophilic sensitizer, mono-L-aspartyl chlorin e6 (NPe6), in a primate model of choroidal neovascularization (CNV).



NPe6 (25 mg/kg) and indocyanine green (ICG, 15 mg/kg) were intravenously administered simultaneously in monkeys with laser-induced CNVs. Eyes were enucleated and examined by fluorescence microscopy immediately, 20 minutes, and 1, 4, and 24 hours after dye injection.



Fluorescence peaked in retinal vessels immediately after dye administration; rapid washout began 1 hour postinjection. In contrast, experimental CNV demonstrated little fluorescence immediately after injection, with increased intensity at later time points. Peak dye fluorescence in CNV occurred 1 hour following dye injection. NPe6 fluorescence diminished rapidly and washed out completely by 24 hours. The dye accumulation and retention pattern of ICG dye resembled that of NPe6.



This study demonstrated the selective accumulation of NPe6 photosensitizer and ICG dye within experimental CNV and rapid clearance from the circulation and retinochoroidal tissue.
Mori
Keisuke
K
Department of Ophthalmology, Saitama Medical University, Iruma, Saitama, Japan.
Kanai
Kaname
K
Peyman
Gholam A
GA
Yoneya
Shin
S
D000818:Animals; D020256:Choroidal Neovascularization / Q000378:metabolism*; D004396:Coloring Agents / Q000493:pharmacokinetics; D004195:Disease Models, Animal; D007208:Indocyanine Green / Q000493:pharmacokinetics; D008251:Macaca; D008856:Microscopy, Fluorescence; D017319:Photosensitizing Agents / Q000493:pharmacokinetics*; D011166:Porphyrins / Q000493:pharmacokinetics*; D014018:Tissue Distribution
D004396:Coloring Agents; D017319:Photosensitizing Agents; D011166:Porphyrins; D007208:Indocyanine Green; C053434:Talaporfin