title: Breed differences in clearance of porcine FSH in hypophysectomized rats.
abstract: Extracts of anterior pituitary (AP) glands were infused i.v. into hypophysectomized male rats followed by sequential sampling of blood for 120 min. Determination of follicle-stimulating hormone (FSH) concentrations established that FSH from Chinese Meishan males decreased in the circulation of rats more slowly than FSH in extracts of AP from crossbred occidental pigs (P<0.003). Additionally, FSH from AP extracts of castrated males disappeared somewhat more slowly (P<0.06) than FSH from extracts of boars. Evaluation of FSH by bioassay and radioimmunoassay yielded similar concentrations in AP from Meishan and crossbred boars. Serum testosterone concentrations increased with time through 90 min after infusion of AP, but the rate of increase of testosterone was not related to amount of luteinizing hormone (LH) that was administered indicating LH receptor saturation. Unexpectedly, the rate of increase in testosterone was more rapid with AP extracts from boars than with extracts from castrated males. Observations from the current study imply structural alterations of FSH in the AP of Meishan males relative to crossbred males allowing sustained concentrations in the circulation, and this FSH possesses similar activation of the FSH receptor. The amount of LH in the AP extracts saturated the LH receptors of the hypophysectomized male rats, but some factor in extracts of boars differed from those of castrated males.
authors.affiliation: Department of Neuroscience and Cell Biology, University of Medicine and Dentistry, Robert Wood Johnson (Rutgers) Medical School, Piscataway, NJ 08854, USA.
authors.forename: G J
authors.initials: GJ
authors.lastname: Macdonald
authors.forename: T H
authors.initials: TH
authors.lastname: Wise
authors.forename: P M
authors.initials: PM
authors.lastname: Sluss
authors.forename: J J
authors.initials: JJ
authors.lastname: Ford
mesh_terms: D000818:Animals; D005640:Follicle Stimulating Hormone / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics*; D007016:Hypophysectomy; D008297:Male; D008657:Metabolic Clearance Rate; D009919:Orchiectomy; D010903:Pituitary Gland, Anterior / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013045:Species Specificity; D013552:Swine; D013739:Testosterone / Q000097:blood; D014020:Tissue Extracts / Q000008:administration & dosage / Q000737:chemistry
chemical_list: D014020:Tissue Extracts; D013739:Testosterone; D005640:Follicle Stimulating Hormone