title: Evaluation of somatic hybrids of potato with Solanum stenotomum after a long-term in vitro conservation.
abstract: Somatic hybrids of potato with a cultivated relative, Solanum stenotomum also called Solanum tuberosum Stenotomum group, were evaluated for their physiological and agronomical characteristics as well as the stability of the introgressed resistance to bacterial wilt, caused by Ralstonia solanacearum, after a long-term in vitro conservation for more than 5 years. Analysis of photosynthesis showed that the PEPC/Rubisco ratio remained lower than 0.5 for all vitroplants of potato and the somatic hybrids, except for the relative species. This indicates that the carbon metabolism is heterotrophic (ratio>1) for S. stenotomum, and autotrophic for potato and the somatic hybrids (ratio<1). In both in vitro and greenhouse conditions, potato and the somatic hybrids produced few bigger tubers, while many small tubers were obtained from the relative. The hybrid tubers were morphologically intermediate. The starch content of hybrid tubers was much lower than that of potato, but similar to that of the relative species. Interestingly, the level of bacterial resistance, introgressed from S. stenotomum into potato, was shown to be very stable and remained as high as that of the relative after a long-term period of in vitro conservation.
authors.affiliation: Université de La Réunion, UMR Peuplements végétaux et bio-agresseurs en milieu tropical, La Réunion, France. isabelle.fock@univ-reunion.fr
authors.forename: Isabelle
authors.initials: I
authors.lastname: Fock
authors.forename: Cécile
authors.initials: C
authors.lastname: Collonnier
authors.forename: Danielle
authors.initials: D
authors.lastname: Lavergne
authors.forename: Sébastien
authors.initials: S
authors.lastname: Vaniet
authors.forename: Annick
authors.initials: A
authors.lastname: Ambroise
authors.forename: Jacques
authors.initials: J
authors.lastname: Luisetti
authors.forename: Hippolyte
authors.initials: H
authors.lastname: Kodja
authors.forename: Darasinh
authors.initials: D
authors.lastname: Sihachakr
mesh_terms: D015153:Blotting, Western; D004171:Diploidy; D006824:Hybridization, Genetic; D010788:Photosynthesis; D010940:Plant Proteins / Q000378:metabolism; D035281:Plant Tubers / Q000254:growth & development / Q000378:metabolism; D043368:Ralstonia solanacearum / Q000254:growth & development; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism; D032322:Solanum / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D011198:Solanum tuberosum / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology
chemical_list: D010940:Plant Proteins; D012273:Ribulose-Bisphosphate Carboxylase