title: Isolation of fatty acids with anticancer activity from Protaetia brevitarsis larva.
abstract: In this study, biologically active compounds were isolated from Protaetia brevitarsis larva (PBL) by dichloromethane extraction. The dichloromethane extract from PBL was highly cytotoxic to various cancer cells. From a silica gel column chromatograpy of this extract, we obtained four fractions (F-2, F-4, F-5 and F-7) having apoptosis-inducing activity. These fractions induced DNA ladder and caspase-3 activation during apoptosis in colon 26 tumor cells. In 1H and 13C NMR and mass spectral analysis of the fraction F-2 showing the highest apoptosis-inducing activity, we found that the fraction was composed of three free fatty acids such as palmitic acid, (Z)-9-octadecenoic acid and octadecenoic acid. These results indicate that the dichloromethane extract of PBL includes anticancer components composed of at least three fatty acids, and apoptosis-inducing activity of the extract was mediated by caspase-3 activation in tumor cells.
authors.affiliation: Department of Biochemistry, College of Medicine, Konyang University, Daejeon 302-801, Korea.
authors.forename: Yung-Choon
authors.initials: YC
authors.lastname: Yoo
authors.forename: Byung-Hoon
authors.initials: BH
authors.lastname: Shin
authors.forename: Jang-Hee
authors.initials: JH
authors.lastname: Hong
authors.forename: Junglim
authors.initials: J
authors.lastname: Lee
authors.forename: Hee-Youn
authors.initials: HY
authors.lastname: Chee
authors.forename: Kyung-Sik
authors.initials: KS
authors.lastname: Song
authors.forename: Kyung-Bok
authors.initials: KB
authors.lastname: Lee
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D001517:Coleoptera / Q000737:chemistry*; D005227:Fatty Acids / Q000302:isolation & purification* / Q000494:pharmacology; D007814:Larva; D051379:Mice
chemical_list: D000970:Antineoplastic Agents; D005227:Fatty Acids; D053148:Caspase 3