title: Cyclic AMP-regulated exocytosis of Escherichia coli from infected bladder epithelial cells.
abstract: The superficial bladder epithelium is a powerful barrier to urine and also serves as a regulator of bladder volume, which is achieved by apical exocytosis of specialized fusiform vesicles during distension of the bladder. We report that type 1 fimbriated uropathogenic Escherichia coli (UPEC) circumvents the bladder barrier by harboring in these Rab27b/CD63-positive and cAMP-regulatable fusiform vesicles within bladder epithelial cells (BECs). Incorporation of UPEC into BEC fusiform compartments enabled bacteria to escape elimination during voiding and to re-emerge in the urine as the bladder distended. Notably, treatment of UPEC-infected mice with a drug that increases intracellular cAMP and induces exocytosis of fusiform vesicles reduced the number of intracellular E. coli.
authors.affiliation: Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA.
authors.forename: Brian L
authors.initials: BL
authors.lastname: Bishop
authors.forename: Mathew J
authors.initials: MJ
authors.lastname: Duncan
authors.forename: Jeongmin
authors.initials: J
authors.lastname: Song
authors.forename: Guojie
authors.initials: G
authors.lastname: Li
authors.forename: David
authors.initials: D
authors.lastname: Zaas
authors.forename: Soman N
authors.initials: SN
authors.lastname: Abraham
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects / Q000502:physiology; D000242:Cyclic AMP / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000502:physiology*; D004927:Escherichia coli Infections / Q000517:prevention & control*; D005089:Exocytosis / Q000187:drug effects*; D006801:Humans; D051379:Mice; D001743:Urinary Bladder / Q000187:drug effects / Q000382:microbiology*; D014552:Urinary Tract Infections / Q000517:prevention & control; D019459:Urothelium / Q000187:drug effects / Q000382:microbiology*
chemical_list: D000242:Cyclic AMP