Injectable gel with synthetic collagen-binding peptide for enhanced osteogenesis in vitro and in vivo.
A synthetic peptide denoted as collagen-binding motif (CBM) was identified from osteopontin (OPN), a multisubunit extracellular matrix (ECM) protein, by enzymatic digestion with chymotrypsin. The aim of this study was to examine the feasibility of identified CBM peptide as an active component of gel type scaffold material in osteogenesis. The binding of CBM peptide to collagen was specific and presented high affinity. Cell adhesion and growth on CBM peptide-immobilized gel were significantly increased as compared with those on gel with control peptide or without peptide. The CBM peptide-immobilized gel increased osteoblastic differentiation, followed by marked bone formation in the rabbit calvarial defect sites at 4 weeks. Taken together, the injectable gel with synthetic CBM peptide has a potential to induce osteogenesis in vitro and in vivo, suggesting its clinical application in bone regeneration procedure.
Lee
Jue-Yeon
JY
Intellectual Biointerface Engineering Center, School of Dentistry, Seoul National University, Seoul, South Korea.
Choo
Jung-Eun
JE
Park
Hyun-Jung
HJ
Park
Jun-Bum
JB
Lee
Sang-Chul
SC
Jo
Inho
I
Lee
Seung-Jin
SJ
Chung
Chong-Pyoung
CP
Park
Yoon-Jeong
YJ
D000464:Alginates / Q000737:chemistry*; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D004337:Drug Carriers / Q000737:chemistry; D005782:Gels / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D007267:Injections; D010012:Osteogenesis / Q000187:drug effects*; D053495:Osteopontin / Q000008:administration & dosage* / Q000737:chemistry; D011817:Rabbits; D012887:Skull Fractures / Q000188:drug therapy* / Q000473:pathology*; D016896:Treatment Outcome
D000464:Alginates; D004337:Drug Carriers; D005782:Gels; D006603:Hexuronic Acids; D053495:Osteopontin; D020723:Glucuronic Acid