Sequence-specific amplification polymorphisms (SSAPs): a multi-locus approach for analyzing transposon insertions.
Multiple copies of transposable elements, inserted at random around the host genome, can be used as molecular markers. Sequence-specific amplification polymorphisms (SSAPs) amplify the region between a PCR primer site near the end of an element and an adjacent restriction site in the flanking genomic DNA. Each amplified insertion is revealed as a band on a sequencing gel or capillary electrophoresis, and a genomic DNA sample produces a characteristic fingerprint of bands. Here, we explain the SSAP marker development method, which can be performed for any species, with recommendations for experimental parameters in several plant species. It takes about 1-2 weeks to complete the whole SSAP marker procedure.
Syed
Naeem H
NH
Plant Research Unit, University of Dundee at SCRI, Invergowrie, Dundee DD2 5DA, UK.
Flavell
Andrew J
AJ
D004251:DNA Transposable Elements; D005819:Genetic Markers; D018745:Genome, Plant; D021141:Nucleic Acid Amplification Techniques; D012150:Polymorphism, Restriction Fragment Length
D004251:DNA Transposable Elements; D005819:Genetic Markers