Alternative procedure for charged derivatization to enhance detection responses of steroids in electrospray ionization-MS.
A derivatization procedure has been examined to enhance the electrospray ionization (ESI)-MS detectabilities of steroids that charged derivatization is not suitable for. The derivatization procedure with 2-hydrazinopyridine or isonicotinoyl azide was very effective for the sensitive detection of di-oxosteroids or di-hydroxysteroids, respectively, and the detection limits of the resulting derivatives were as low as about 2 fmol. The derivatives also provided intense characteristic product ions in the MS-MS, which are expected to be usable for the selected reaction monitoring mode.
Higashi
Tatsuya
T
Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Japan. higashi@p.kanazawa-u.ac.jp
Nishio
Tadashi
T
Hayashi
Noriko
N
Shimada
Kazutake
K
D002853:Chromatography, Liquid; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013256:Steroids / Q000032:analysis*; D053719:Tandem Mass Spectrometry
D013256:Steroids