Regulation of human umbilical cord blood-derived multi-potent stem cells by autogenic osteoclast-based niche-like structure.
Stem cell niches provide the micro-environment for the development of stem cells. Under our culturing regimen, a kind of osteoclast-centralized structure supports the proliferation of MSCs, derived from human cord blood, once they reside on osteoclasts. MSCs in this structure expressed Oct4 which is a marker of embryonic stem cells. Floating daughter cells of MSCs colony showed abilities to differentiate into osteocyte, adipocyte, and neuronal progenitor cells. Compared with the easy senescence of MSCs without this niche-like structure in vitro, these results suggested that osteoclasts might play an important role the development and maintenance of Umbilical cord blood (UCB)-derived MSCs and might provide a means to expand UCB-MSCs in vitro, more easily, through a stem cell niche-like structure.
Sun
Bo
B
Laboratory of Stem Cell and Tumor Biology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Republic of Korea.
Jeong
Yun-Hyeok
YH
Jung
Ji-Won
JW
Seo
Kwangwon
K
Lee
Yong-Soon
YS
Kang
Kyung-Sun
KS
D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods; D016207:Cytokines / Q000378:metabolism; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000502:physiology; D010010:Osteoclasts / Q000166:cytology* / Q000502:physiology; D023822:Tissue Engineering / Q000379:methods*
D016207:Cytokines