title: Inhibition of anodic galvanotaxis of green paramecia by T-type calcium channel inhibitors.
abstract: Calcium ion (Ca2+) is one of the key regulatory elements for ciliary movements in the Paramecium species. It has long been known that members of Paramecium species including green paramecia (Paramecium bursaria) exhibit galvanotaxis which is the directed movement of cells toward the anode by swimming induced in response to an applied voltage. However, our knowledge on the mode of Ca2+ action during green paramecia anodic galvanotactic response is still largely limited. In the present study, quantification of anodic galvanotaxis was carried out in the presence and absence of various inhibitors of calcium signaling and calcium channels. Interestingly, galvanotactic movement of the cells was completely inhibited by a variety of Ca2+-related inhibitors. Such inhibitors include a Ca2+ chelator (EGTA), general calcium channel blockers (such as lanthanides), inhibitors of intracellular Ca2+ release (such as ruthenium red and neomycin), and inhibitors of T-type calcium channels (such as NNC 55-0396, 1-octanol and Ni2+). However, L-type calcium channel inhibitors such as nimodipine, nifedipine, verapamil, diltiazem and Cd2+ showed no inhibitory action. This may be the first implication for the involvement of T-type calcium channels in protozoan cellular movements.
authors.affiliation: Faculty and Graduate School of Environmental Engineering, The University of Kitakyushu, Kitakyushu 808-0135, Japan.
authors.forename: Miki
authors.initials: M
authors.lastname: Aonuma
authors.forename: Takashi
authors.initials: T
authors.lastname: Kadono
authors.forename: Tomonori
authors.initials: T
authors.lastname: Kawano
mesh_terms: D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology* / Q000633:toxicity; D020747:Calcium Channels, T-Type / Q000187:drug effects / Q000502:physiology*; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D002614:Chelating Agents / Q000494:pharmacology / Q000633:toxicity; D004594:Electrophysiology; D009355:Neomycin / Q000494:pharmacology; D010247:Paramecium / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D012430:Ruthenium Red / Q000494:pharmacology; D019284:Thapsigargin / Q000494:pharmacology
chemical_list: D002121:Calcium Channel Blockers; D020747:Calcium Channels, T-Type; D002614:Chelating Agents; D012430:Ruthenium Red; D019284:Thapsigargin; D009355:Neomycin