Diarylheptanoids from Alnus hirsuta inhibit the NF-kB activation and NO and TNF-alpha production.
Six diarylheptanoids (1-6) from the stem bark of Alnus hirsuta were investigated for their inhibitory activity against LPS-induced NF-kB activation and NO and TNF-alpha production. Among them, compounds 2, 3, and 6 displayed inhibitory activity against NF-kB activation and NO and TNF-alpha production with IC50 values of 9.2-9.9 microM, 18.2-19.3 microM, and 22.3-23.7 microM, respectively, in RAW264.7 cells. Three active compounds had no significant cytotoxicity in RAW264.7 cells at their effective concentrations. This is the first report of NF-kB-inhibitory activity of these compounds and supports the pharmacological use of A. hirsuta, which has been employed as a herbal medicine for the treatment of inflammatory diseases.
Jin
WenYi
W
College of Pharmacy, Chungnam National University, Korea.
Cai
Xing Fu
XF
Na
MinKyun
M
Lee
Jung Joon
JJ
Bae
KiHwan
K
D029661:Alnus / Q000737:chemistry*; D000818:Animals; D002460:Cell Line; D036381:Diarylheptanoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D000432:Methanol / Q000737:chemistry; D051379:Mice; D015394:Molecular Structure; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D009569:Nitric Oxide / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000096:biosynthesis
D036381:Diarylheptanoids; D016328:NF-kappa B; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D000432:Methanol