title: Poly(lactic-co-glycolic acid) microspheres for the controlled release of huperzine A: in vitro and in vivo studies and the application in the treatment of the impaired memory of mice.
abstract: Huperzine A loaded poly(D,L-lactic-co-glycolic acid) (PLGA) microspheres were prepared by an oil/water (o/w) solvent evaporation technique. With a decrease of the ratio of o/w from 1 : 100 to 1 : 50, the encapsulation efficiency was reduced about 4%. Increasing the PVA concentration from 0.5 to 2% reduced the percentage encapsulation efficiency of huperzine A from 60.7 to 47.4% and the particle size of microspheres from 84.2 to 26.2 microm. The addition of stearic acid improved the encapsulation efficiency, but also accelerated the in vitro release of hupezine A from microspheres. After i.m. administration of huperzine A loaded microspheres in mice, huperzine A was sustained released from the PLGA microspheres up to 12 d with a low initial burst. Passive avoidance test of mice showed that the microspheres formulation offered an improved therapeutic efficiency in the treatment of the impaired memory of the mice superior to injection gastric (i.g.) administration of huperzine A suspension at the same dose, whose therapeutic efficiency was similar as that of a 50% reduced dose of the microspheres formulation.
authors.affiliation: College of Life Science, Jilin University, Changchun, China.
authors.forename: Dafeng
authors.initials: D
authors.lastname: Chu
authors.forename: Jingwei
authors.initials: J
authors.lastname: Tian
authors.forename: Wanhui
authors.initials: W
authors.lastname: Liu
authors.forename: Zi
authors.initials: Z
authors.lastname: Li
authors.forename: Youxin
authors.initials: Y
authors.lastname: Li
mesh_terms: D000470:Alkaloids; D000818:Animals; D002800:Cholinesterase Inhibitors / Q000008:administration & dosage*; D019344:Lactic Acid / Q000008:administration & dosage*; D008569:Memory Disorders / Q000188:drug therapy*; D051379:Mice; D008863:Microspheres; D011100:Polyglycolic Acid / Q000008:administration & dosage*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000008:administration & dosage*; D012717:Sesquiterpenes / Q000008:administration & dosage*
chemical_list: D000470:Alkaloids; D002800:Cholinesterase Inhibitors; D011108:Polymers; D012717:Sesquiterpenes; C050426:huperzine A; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid