title: Divergent in vitro and in vivo lipid peroxidation in the postprandial phase of patients with type I diabetes mellitus.
abstract: OBJECTIVE
  The two- to fourfold higher risk of cardiovascular disease in diabetes mellitus is more strongly predicted by the postprandial than by the fasting blood glucose and lipids. We aimed to investigate the impact of postprandial changes in serum lipoprotein fractions on lipid peroxidation in type I diabetes mellitus (T1DM).
  
  
  METHODS
  This was a prospective observational study.
  
  
  METHODS
  The study was performed at Antwerp University Hospital, Belgium.
  
  
  METHODS
  Twenty-three well-controlled T1DM patients were included.
  
  
  METHODS
  Patients received a standard breakfast and lunch (>50% energy as fat). Blood was sampled at fasting (F), after the post-breakfast hyperglycemic peak (BP), just before lunch (B), after the post-lunch hyperglycemic peak (LP), after the post-lunch dale (LD) and 5 h after lunch (L) for the measurement of serum lipids, lipoprotein subfraction composition, alpha-tocopherol and lipid peroxidation in vivo and in vitro.
  
  
  RESULTS
  Serum triacylglycerols (Tgs) increased (from 1.03+/-0.40 at F to 1.60+/-0.87 mmol/l at LP, P=0.001), but cholesterol decreased by 12% in parallel with alpha-tocopherol (from 4.43+/-0.76 at F to 4.12+/-0.82 micromol/mmol total lipid at B, P=0.006). Although plasma malondialdehyde increased from 1.02+/-0.36 at F to 1.14+/-0.40 micromol/L at LP, P=0.03, copper-induced in vitro peroxidation decreased in the low-density lipoprotein and high-density lipoprotein fractions.
  
  
  CONCLUSIONS
  In well-controlled T1DM patients moderate postprandial increases in serum Tgs are accompanied by a relative deficiency in alpha-tocopherol. Lipid peroxidation in vivo increases but cannot be ascribed to changes in the susceptibility of lipoproteins to copper-induced in vitro peroxidation.
authors.affiliation: Metabolic Research Unit, University of Antwerp, Antwerp, Belgium. begona.manuelykeenoy@ua.ac.be
authors.forename: B
authors.initials: B
authors.lastname: Manuel-Y-Keenoy
authors.forename: C
authors.initials: C
authors.lastname: de Vos
authors.forename: A
authors.initials: A
authors.lastname: van Campenhout
authors.forename: M
authors.initials: M
authors.lastname: Vinckx
authors.forename: P
authors.initials: P
authors.lastname: Abrams
authors.forename: C
authors.initials: C
authors.lastname: van Campenhout
mesh_terms: D000328:Adult; D019540:Area Under Curve; D002784:Cholesterol / Q000097:blood; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000378:metabolism*; D005215:Fasting / Q000097:blood; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation; D008055:Lipids / Q000097:blood*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D018384:Oxidative Stress; D019518:Postprandial Period; D011446:Prospective Studies; D014280:Triglycerides / Q000097:blood; D024502:alpha-Tocopherol / Q000097:blood
chemical_list: D008055:Lipids; D014280:Triglycerides; D008315:Malondialdehyde; D002784:Cholesterol; D024502:alpha-Tocopherol