[The expression of transforming growth factor beta1 and its I, II receptors in the development of rat embryo and embryonic lung].
OBJECTIVE
To investigate the expression of TGFbeta1, TbetaRI, TbetaRII in the development of rat embryo and embryonic lung and discuss the interrelationship and function mechanisms between them.


METHODS
Half-quantity RT-PCR and immunohistochemical staining were performed in studying these changes.


RESULTS
Half-quantity RT-PCR demonstrated that the expressions of these three factors increased in the 13th-15th days and decreased in the 16th-17th days of the embryonic development. Immunohistochemical staining showed that TGFbeta1 was mainly expressed in the developing bronchus epithelial cells.Its receptors were obviously expressed in primitive pulmonary alveolus.


CONCLUSIONS
TGFbeta1 and its receptors play an important regulatory role in the development of rat embryo and embryonic lung, especially in organic morphodifferentiation.
Guo
Qiong
Q
Department of Human Anatomy, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Wang
Fan
F
Lu
Chang-Qing
CQ
Li
Li-Ying
LY
D000818:Animals; D005260:Female; D007150:Immunohistochemistry; D008168:Lung / Q000196:embryology* / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D053773:Transforming Growth Factor beta1 / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology
D018125:Receptors, Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1