Immune responses of pigs inoculated with a recombinant fowlpox virus coexpressing GP5/GP3 of porcine reproductive and respiratory syndrome virus and swine IL-18.
Two recombinant fowlpox viruses (rFPV-ORF5-ORF3 and rFPV-IL-18-ORF5-ORF3) containing the ORF5/ORF3 cDNAs of PRRSV (strain Chang Chun) and IL-18 of swine were constructed and evaluated for theirs abilities to induce humoral and cellular responses in piglets. In addition, their abilities to protect piglets against homologous virus challenge were examined. All piglets were given booster vaccinations at 21 days after the initial inoculation, and all piglets were challenged at 60 after the initial inoculation. Control groups were inoculated with wild-type fowlpox virus (wtFPV). All animals vaccinated with rFPV-ORF5-ORF3 and rFPV-IL-18-ORF5-ORF3 developed specific anti-PRRSV ELISA antibody and neutralizing antibody, as well as T-lymphocyte proliferation response. To evaluate the cellular immune function, IFN-gamma production in pigs serum and T-lymphocytes (CD4 and CD8 T cells) in peripheral blood were examined. Following challenge with a pathogenic strain of PRRSV (strain Chang Chun), piglets inoculated with recombinant fowlpox virus (rFPV) showed lower (P<0.05) temperature, viremia and virus load in bronchial lymph nodes than control animals, suggesting the establishment of partial protection against PRRSV infection. The results demonstrated the potential use of a fowlpox virus-based recombinant vaccine in the control and prevention of PRRSV infections.
Shen
Guoshun
G
Genetic Engineering Laboratory, Academy of Military Medical Sciences, Changchun 130062, PR China.
Jin
Ningyi
N
Ma
Mingxiao
M
Jin
Kuoshi
K
Zheng
Min
M
Zhuang
Tianzhong
T
Lu
Huijun
H
Zhu
Guangze
G
Jin
Hongtao
H
Jin
Minglan
M
Huo
Xiaowei
X
Qin
Xiaoguang
X
Yin
Ronglan
R
Li
Chang
C
Li
Hongwen
H
Li
Yang
Y
Han
Zhenzhen
Z
Chen
Yifeng
Y
Jin
Miaomiao
M
D000276:Adjuvants, Immunologic / Q000235:genetics; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001831:Body Temperature; D049109:Cell Proliferation; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005587:Fowlpox virus / Q000235:genetics / Q000276:immunology*; D007117:Immunization, Secondary; D007371:Interferon-gamma / Q000096:biosynthesis / Q000097:blood; D020382:Interleukin-18 / Q000235:genetics / Q000276:immunology*; D008198:Lymph Nodes / Q000821:virology; D009500:Neutralization Tests; D019318:Porcine Reproductive and Respiratory Syndrome / Q000276:immunology* / Q000517:prevention & control*; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics / Q000276:immunology*; D013552:Swine; D013601:T-Lymphocytes / Q000276:immunology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*; D014766:Viremia
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D020382:Interleukin-18; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C114789:glycoprotein 5, PRRSV; D007371:Interferon-gamma