Informativeness of dinucleotide repeat-based primers in fungal pathogen of rice Magnaporthe grisea.
Microsatellites or simple sequence repeats (SSRs) occur ubiquitously and show complex patterns in length, motif size and sequence. Among SSRs, dinucleotide repeats occur in high abundance in fungi with shorter length as compared to other organisms. In this study, multilocus profiles obtained in Magnaporthe grisea, a model plant pathogen were evaluated. The results showed lower rate of polymorphism by (GT)(n)/(TG)(n) repeat-based primers and suggested occurrence of (GA)(n)/(AG)(n) repeats as integral repeats and (TC)(n)/(CT)(n) and (AC)(n)/(CA)(n) as non-integral repeats. Low repeat length variation was found to be correlated with less number of repeat motifs. The study provides an insight into the possibility of molecular coevolution of mobile elements and dinucleotide repeats in fungi. The study could be applied to other species for wider applications including evolutionary and population genetics.
Chadha
Sonia
S
Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, India. soniachaddha@yahoo.com
Gopalakrishna
T
T
D000818:Animals; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000235:genetics*; D019143:Evolution, Molecular; D005838:Genotype; D020082:Magnaporthe / Q000145:classification* / Q000235:genetics*; D018895:Microsatellite Repeats; D012275:Oryza / Q000382:microbiology; D011110:Polymorphism, Genetic
D004271:DNA, Fungal