title: Quantification of F2-isoprostanes as a biomarker of oxidative stress.
abstract: Oxidant stress has been implicated in a wide variety of disease processes. One method to quantify oxidative injury is to measure lipid peroxidation. Quantification of a group of prostaglandin F(2alpha)-like compounds derived from the nonezymatic oxidation of arachidonic acid, termed the F2-isoprostanes (F2-IsoPs), provides an accurate assessment of oxidative stress both in vitro and in vivo. In fact, in a recent independent study sponsored by the National Institutes of Health (NIH), F2-IsoPs were shown to be the most reliable index of in vivo oxidant stress when compared against other well known biomarkers. This protocol details our laboratory's method to quantify F2-IsoPs in biological fluids and tissues using gas chromatography-mass spectrometry (GC-MS). This procedure can be completed for 12-15 samples in 6-8 h.
authors.affiliation: Division of Clinical Pharmacology, Vanderbilt University School of Medicine, Nashville, Tennessee, 37232-6602, USA.
authors.forename: Ginger L
authors.initials: GL
authors.lastname: Milne
authors.forename: Stephanie C
authors.initials: SC
authors.lastname: Sanchez
authors.forename: Erik S
authors.initials: ES
authors.lastname: Musiek
authors.forename: Jason D
authors.initials: JD
authors.lastname: Morrow
mesh_terms: D015415:Biomarkers / Q000032:analysis*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D028441:F2-Isoprostanes / Q000032:analysis* / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D018384:Oxidative Stress
chemical_list: D015415:Biomarkers; D028441:F2-Isoprostanes