[Study on the comparison of rDNA and ITS sequence of Fumonisin-producing strains].
OBJECTIVE
To study the phylogenetic relationships of fumonisin-producing Fusarium verticillioides isolates from China and Gibberella fujikuroi species complex.


METHODS
5.8S, 28S rDNA and ITS1 and ITS2 sequences of 4 ATCC typical strains and 12 isolates from different geographical regions and different samples in China and other countries were determined and analyzed. The results were submitted to GenBank. Phylogenetic tree and homology tree were illustrated. The characteristics of etiological distribution of toxigenetic Fusarium verticillioides and the relativity of of rDNA ITS2 sequence and the fumS gene was elucidated by comparing the isolates from China, South Africa and USA.


RESULTS
It was found that rDNA highly devergent ITS2 sequences of our isolates with positive fumonisin biosynthase gene were type I, which was consistant with fumonisin-producing isolates from South Africa. The Fusarium verticillioides isolates with ITS2 type II sequence were fumS gene negative. Two Fusarium subglutinans isolates SD-fmv047 and SD-fmv049 were nonfumonisin-producing and ITS2 sequences were neither type I nor II. The DNA sequences had high homology with Fusarium oxysporum.


CONCLUSIONS
New and reliable theory and technology supports were provided for the further study of phylogenetic relationships of Gibberella fujikuroi species complex.
Wang
Xiaoying
X
Institute of Nutrition and Food safety, Chinese Center for Disease Control and Food Safety, Beijing 100050, China.
Liu
Xiumei
X
D001483:Base Sequence; D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D037341:Fumonisins / Q000032:analysis*; D005670:Fusarium / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010802:Phylogeny; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D017422:Sequence Analysis, DNA; D003313:Zea mays / Q000382:microbiology
D017931:DNA Primers; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D037341:Fumonisins; D012339:RNA, Ribosomal, 28S