A unifying concept of trophoblastic differentiation and malignancy defined by biomarker expression.
Several trophoblast phenotypes, including cytotrophoblast, syncytiotrophoblast, and extravillous trophoblast, emerge during gestation. To clarify the lineage relationship between these subtypes, we profiled p63 localization in developing and term placental tissue, as well as in trophoblastic tumors, using antibodies specific to full-length (TAp63) and one against all p63 isoforms (TAp63 and DeltaNp63). Localization of p63 was compared with that of biomarkers of proliferation and trophoblastic differentiation, including mib-1, inhibin, and MelCAM. In early gestation, p63 was localized principally to villous cytotrophoblast after contact with the villous mesenchyme, absent in the trophoblast columns, and early implantation trophoblast. In the maturing placenta, intraplacental perivillous fibrin correlated with the emergence of a p63-positive "transitional" (vacuolated) extravillous trophoblast from cytotrophoblast, which differentiated further into a "mature" p63-negative extravillous trophoblast. The same lineage pathway emerged from entrapped villi on the chorionic membrane. Virtually all p63 immunopositivity was attributed to dominant-negative p63. The immunophenotypic patterns seen in the immature and mature placenta permit the resolution of all trophoblastic phenotypes within 3 lineage pathways of cytotrophoblast differentiation, including cytotrophoblast-to-trophoblast column/implantation site, cytotrophoblast-to-syncytiotrophoblast, and cytotrophoblast-to-mature extravillous trophoblast. In the latter pathway, a transitional (vacuolated) p63-positive extravillous trophoblast emerges from and links cytotrophoblast to mature extravillous trophoblast in intraplacental fibrin, chorionic membrane, and basal plate. The placental trophoblast is thus resolved within this continuum of differentiation. Terms such as transitional and mature extravillous trophoblast are proposed to reflect the differentiation phases of this unique epithelium. p63 staining patterns in trophoblastic tumors reflect timing of neoplastic transformation during trophoblastic differentiation.
Lee
Yonghee
Y
Division of Women's and Perinatal Pathology, Department of Pathology, Brigham and Women's Hospital, Boston, MA 02115, USA; Department of Pathology, College of Medicine, Pochon CHA University, Bundang CHA General Hospital, Sungnam City, Kyonggi-do 463-712, South Korea.
Kim
Kyu-Rae
KR
Department of Pathology, University of Ulsan College of Medicine, Asan Medical Center, Seoul 138-736, Korea.
McKeon
Frank
F
Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
Yang
Annie
A
Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
Boyd
Theonia K
TK
Department of Pathology, Children's Hospital, Boston, MA 02115, USA.
Crum
Christopher P
CP
Division of Women's and Perinatal Pathology, Department of Pathology, Brigham and Women's Hospital, Boston, MA 02115, USA.
Parast
Mana M
MM
Division of Women's and Perinatal Pathology, Department of Pathology, Brigham and Women's Hospital, Boston, MA 02115, USA. Electronic address: mparast@partners.org.
D015415:Biomarkers / Q000032:analysis; D051929:CD146 Antigen / Q000032:analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007265:Inhibins / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis*; D011247:Pregnancy; D014328:Trophoblastic Neoplasms / Q000378:metabolism*; D014327:Trophoblasts / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases / Q000032:analysis
D015415:Biomarkers; D051929:CD146 Antigen; C081876:CKAP4 protein, human; D008565:Membrane Proteins; D007265:Inhibins; C513496:MIB1 ligase, human; D044767:Ubiquitin-Protein Ligases