title: Modular mass spectrometric tool for analysis of composition and phosphorylation of protein complexes.
abstract: The combination of high accuracy, sensitivity and speed of single and multiple-stage mass spectrometric analyses enables the collection of comprehensive sets of data containing detailed information about complex biological samples. To achieve these properties, we combined two high-performance matrix-assisted laser desorption ionization mass analyzers in one modular mass spectrometric tool, and applied this tool for dissecting the composition and post-translational modifications of protein complexes. As an example of this approach, we here present studies of the Saccharomyces cerevisiae anaphase-promoting complexes (APC) and elucidation of phosphorylation sites on its components. In general, the modular concept we describe could be useful for assembling mass spectrometers operating with both matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) ion sources into powerful mass spectrometric tools for the comprehensive analysis of complex biological samples.
authors.affiliation: Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, California, United States of America.
authors.forename: Justin D
authors.initials: JD
authors.lastname: Blethrow
authors.forename: Chao
authors.initials: C
authors.lastname: Tang
authors.forename: Changhui
authors.initials: C
authors.lastname: Deng
authors.forename: Andrew N
authors.initials: AN
authors.lastname: Krutchinsky
mesh_terms: D000595:Amino Acid Sequence; D008969:Molecular Sequence Data; D010766:Phosphorylation; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D029701:Saccharomyces cerevisiae Proteins