A paramagnetic nanoprobe to detect tumor cell death using magnetic resonance imaging.
A 110 kDa (ca. 5 nm in diameter) bivalent paramagnetic nanoprobe for detecting cell death using magnetic resonance imaging (MRI) is described, in which two biotinylated C2A domains of the protein synaptotagmin-I were complexed with a single avidin molecule, which had been labeled with gadolinium chelates. This nanoprobe bound with high affinity and specificity to the phosphatidylserine exposed by dying cells and was demonstrated to allow MRI detection of apoptotic tumor cells in vitro.
Neves
André A
AA
Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1GA, U.K.
Krishnan
Anant S
AS
Kettunen
Mikko I
MI
Hu
De-En
DE
Backer
Maaike M de
MM
Davletov
Bazbek
B
Brindle
Kevin M
KM
D000818:Animals; D001360:Avidin / Q000737:chemistry; D016923:Cell Death; D045744:Cell Line, Tumor; D005682:Gadolinium / Q000737:chemistry; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D015335:Molecular Probes; D009369:Neoplasms / Q000473:pathology*; D050863:Synaptotagmin I / Q000737:chemistry
D015335:Molecular Probes; D050863:Synaptotagmin I; D001360:Avidin; D005682:Gadolinium