[Preparation of cisplatin-impregnated coral hydroxyapatite drug delivery system].
OBJECTIVE
To prepare a cisplatin-impregnated coral-derived hydroxyapatite (CCHA) drug delivery system (DDS), and evaluate its inhibitory effect on human osteosarcoma cells U-2 OS, human breast cancer and prostatic carcinoma cells PC-3 in vitro.


METHODS
The coral-derived hydroxyapatite (CHA) was manufactured by hydrothermal exchange and impregnated with cisplatin by vacuum freeze-drying techniques. The leaching solutions of this DDS was collected at different intervals in a course of 8 weeks and their inhibitory effect on the cells was tested in vitro by MTT assay.


RESULTS
Electron microscope showed that cisplatin was distributed homogeneously in the pores of CHA. The inhibition rates of the leaching solution on all the tumor cells exceeded 50% except for PC-3 cells, whose inhibition rate was 29.92% when treated with the solution collected at the eighth week.


CONCLUSIONS
CCHA allows sustained drug release and maintains excellent inhibitory effect on human bone tumor cells within 8 weeks in vitro.
Yang
Jin-cheng
JC
Department of Orthopedic Surgery, General Hospital of Guangzhou Command, Guangzhou 510010, China. ycheng75@sohu.com
Yin
Qing-shui
QS
Lin
Jun
J
Huang
Hua-yang
HY
Ding
Huan-wen
HW
Zhang
Yu
Y
Li
Jian
J
D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D004339:Drug Compounding / Q000379:methods; D016503:Drug Delivery Systems; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D008854:Microscopy, Electron; D013997:Time Factors
D000970:Antineoplastic Agents; D017886:Durapatite; D002945:Cisplatin