Identification of an emerging pathogen, Mycobacterium massiliense, by rpoB sequencing of clinical isolates collected in the United States.
Mycobacterium massiliense is a rapidly growing mycobacterium that is indistinguishable from Mycobacterium chelonae/M. abscessus by partial 16S rRNA gene sequencing. We sequenced rpoB, sodA, and hsp65 genes from isolates previously identified as being M. chelonae/M. abscessus and identified M. massiliense from isolates from two patients with invasive disease representing the first reported cases in the United States.
Simmon
Keith E
KE
Associated Regional and University Pathologists (ARUP) Instititue for Clincal and Experimental Pathology, Salt Lake City, UT 84108, USA. cathy.petti@aruplab.com
Pounder
June I
JI
Greene
John N
JN
Walsh
Frank
F
Anderson
Clint M
CM
Cohen
Samuel
S
Petti
Cathy A
CA
D000328:Adult; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D018834:Chaperonin 60; D018833:Chaperonins / Q000235:genetics; D021821:Communicable Diseases, Emerging / Q000453:epidemiology* / Q000382:microbiology; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D009164:Mycobacterium Infections / Q000453:epidemiology* / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014481:United States / Q000453:epidemiology
D001426:Bacterial Proteins; D018834:Chaperonin 60; D012336:RNA, Ribosomal, 16S; C074119:heat-shock protein 65, Mycobacterium; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; D018833:Chaperonins