Cytotoxic withanolides from Tubocapsicum anomalum.
Fifteen new withanolides (1-8, 11-17) and two known withanolides, withanolide D (9) and 17alpha-hydroxywithanolide D (10), were isolated from the stems, roots, and leaves of Tubocapsicum anomalum using bioassay-directed fractionation. The structures were determined by spectroscopic and chemical methods, and the absolute configurations were established by CD analysis and by the Mosher ester method. The structure of 1 and 3 were further confirmed by X-ray crystallographic analysis. Compounds 1, 4-6, 8-10, and 13 showed significant cytotoxic activity against Hep G2, Hep 3B, A-549, MDA-MB-231, MCF-7, and MRC-5 cell lines.
Hsieh
Pei-Wen
PW
Graduate Institute of Natural Products, School of Pharmcy, Kaohsiung Medical University, Kaohsiung 807, Taiwan, Republic of China.
Huang
Zih-You
ZY
Chen
Jyun-Hong
JH
Chang
Fang-Rong
FR
Wu
Ching-Chung
CC
Yang
Yu-Liang
YL
Chiang
Michael Y
MY
Yen
Ming-Hon
MH
Chen
Shu-Li
SL
Yen
Hsin-Fu
HF
Lübken
Tilo
T
Hung
Wen-Chun
WC
Wu
Yang-Chang
YC
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018360:Crystallography, X-Ray; D004354:Drug Screening Assays, Antitumor; D004875:Ergosterol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D008968:Molecular Conformation; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D019657:Solanaceae / Q000737:chemistry*; D013624:Taiwan; D054358:Withanolides
D000972:Antineoplastic Agents, Phytogenic; D054358:Withanolides; C010376:withanolide D; D004875:Ergosterol