title: Organic solvents mediate self-assembly of GAV-9 peptide on mica surface.
abstract: Self-assembly of peptides into fibrils and other morphologies has attracted much attention in many fields, especially in nanofabrication, pathology and biochemistry. In this paper, self-assembly of GAV-9 peptide in organic solvents, ethanol and acetone, was investigated using atomic force microscopy (AFM) and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR). The results indicated that GAV-9 self-assembled into various nanostructures in both solvents after deposited and evaporated on mica. Fibrils with beta-sheet conformation were observed in both solvents when the peptide concentration was higher than 280 microM. However, ordered fibrils with beta-sheet conformation were formed in ethanol, but not in acetone, with a peptide concentration ranging from 7 microM to 28 microM. We attribute the formation of various nanostructures to the different physicochemical properties of the polar organic solvents on the self-assembly of GAV-9 peptide.
authors.affiliation: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jianhua
authors.initials: J
authors.lastname: He
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
mesh_terms: D000096:Acetone / Q000737:chemistry; D000538:Aluminum Silicates / Q000737:chemistry*; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000682:Amyloid / Q000737:chemistry; D016384:Consensus Sequence; D000431:Ethanol / Q000737:chemistry; D018625:Microscopy, Atomic Force; D008958:Models, Molecular; D049329:Nanostructures / Q000737:chemistry*; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry*; D010455:Peptides; D011328:Prions / Q000737:chemistry; D017433:Protein Structure, Secondary; D012997:Solvents / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D051843:Synucleins / Q000737:chemistry
chemical_list: D000538:Aluminum Silicates; D000682:Amyloid; C521774:GAV-9 peptide; D009842:Oligopeptides; D010455:Peptides; D011328:Prions; D012997:Solvents; D051843:Synucleins; D000096:Acetone; D000431:Ethanol; C011934:mica