Development of a large scale process for the conversion of polysialogangliosides to monosialotetrahexosylganglioside with a novel strain of Brevibacterium casei producing sialidase.
A bioconversion process of producing GM1 (monosialotetrahexosylganglioside) on an industrial scale was developed with a novel sialidase-producing strain Brevibacterium casei. The sialidase hydrolyzed polysialogangliosides to produce GM1 but did not act on GM1. When Brevibacterium casei was cultured in a synthetic medium containing crude pig brain gangliosides (10% w/v) at 30 degrees C for 24 h in a 50 l fermenter, most of the polysialogangliosides were converted to GM1. The content of GM1 was increased from 9% in crude gangliosides to 45% with 70% (w/w) yield.
Peng
Yan-Feng
YF
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Wang
Xue-Dong
XD
Wei
Dong-Zhi
DZ
D000818:Animals; D001921:Brain / Q000378:metabolism; D001951:Brevibacterium / Q000201:enzymology / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D005732:Gangliosides / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009439:Neuraminidase / Q000378:metabolism*; D013379:Substrate Specificity; D013552:Swine; D013696:Temperature
D005732:Gangliosides; C025447:sialogangliosides; C016481:trisialoganglioside GT1; D009439:Neuraminidase