title: Potential inhibition of demineralization in vitro by fluoride-releasing sealants.
abstract: BACKGROUND
  The incorporation of fluoride into sealants has been viewed as a viable way to prevent pit-and-fissure caries by potential inhibition of demineralization through the release of fluoride to enamel. The authors conducted a study to examine the effect of a recently introduced fluoride-releasing sealant (ProSeal, Reliance Orthodontic Products, Itasca, Ill.) on enamel demineralization in an in vitro artificial caries system.
  
  
  METHODS
  The authors randomly assigned 45 extracted human third molars to three treatment groups receiving either conventional sealant without fluoride (Group 1), fluoride-releasing sealant (Group 2) or glass ionomer sealant with high fluoride release (Group 3). They placed cavity preparations on the buccal surfaces of the molars and filled them with the assigned material. They placed acid-resistant varnish on the specimens' enamel surfaces to within 1 millimeter of the sealant, leaving a 1-mm rim of sound enamel available for in vitro enamel caries formation. They thermocycled the teeth (500 cycles) in artificial saliva. They subjected the teeth to an in vitro artificial caries challenge for six weeks to produce caries-like lesions in enamel adjacent to the sealant materials. The authors took longitudinal sections from each tooth, immersed them in water and examined them via polarized light microscopy to determine wall lesion frequencies.
  
  
  RESULTS
  The mean (+/- standard deviation) lesion depths were 232 +/- 17 micrometers for Group 1, 144 +/- 21 mum for Group 2 and 128 +/- 15 mum for Group 3. The wall lesion frequency was 12 percent for Group 1 and 7 percent for both Groups 2 and 3. There was a significant difference (P < .05) among the fluoride-releasing materials versus the nonfluoride-releasing material. This study indicates that the new fluoride-releasing sealant substantially reduces the amount of enamel demineralization adjacent to the material.
  
  
  CONCLUSIONS
  ProSeal provided increased demineralization inhibition compared with a conventional sealant containing no fluoride, but less than that shown by a glass ionomer sealant.
  
  
  CONCLUSIONS
  ProSeal's physical properties and cariostatic effects may allow for applications beyond traditional sealant use.
authors.affiliation: College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, FL 33328, USA.
authors.forename: David V
authors.initials: DV
authors.lastname: Salar
authors.forename: Franklin
authors.initials: F
authors.lastname: García-Godoy
authors.forename: Catherine M
authors.initials: CM
authors.lastname: Flaitz
authors.forename: M John
authors.initials: MJ
authors.lastname: Hicks
mesh_terms: D000704:Analysis of Variance; D002327:Cariostatic Agents / Q000627:therapeutic use*; D005459:Fluorides / Q000627:therapeutic use*; D006801:Humans; D010895:Pit and Fissure Sealants / Q000737:chemistry / Q000627:therapeutic use*; D017001:Tooth Demineralization / Q000188:drug therapy* / Q000517:prevention & control
chemical_list: D002327:Cariostatic Agents; D010895:Pit and Fissure Sealants; D005459:Fluorides