[Amplification of proviral DNA of bovine immunodeficiency virus in experimentally infected animals].
The primer set for detection of bovine immunodeficiency virus (BIV) proviral DNA, retrovirus of unknown pathology, by standard polymerase chain reaction was developed. Amplicon of the expected size was detected in DNA from peripheral blood mononuclear cells of seropositive experimentally BIV infected sheep and cattle. Primers targeted the short fragment of BIV pol gene. Sequences of BIV proviral DNA extracted from BIV infected cell culture as well as from experimentally infected cows were taken for targeted pol BI BPX gene locus. Problems of BIV detection from the clinical material of the experimentally and naturally infected animals are discussed.
Lymans'ka
O Iu
OIu
Rola
M
M
Byka
L
L
Kuzmak
Ia
Ia
Volians'kyĭ
Iu L
IuL
Puhach
N B
NB
Novikov
S V
SV
Lymans'kyĭ
O P
OP
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology; D017931:DNA Primers / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D004195:Disease Models, Animal; D016087:Immunodeficiency Virus, Bovine / Q000235:genetics / Q000302:isolation & purification*; D016180:Lentivirus Infections / Q000662:veterinary / Q000821:virology*; D008214:Lymphocytes / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D011533:Proviruses / Q000235:genetics / Q000302:isolation & purification*; D012756:Sheep; D012757:Sheep Diseases / Q000821:virology
D017931:DNA Primers; D004279:DNA, Viral