title: Development of a RP-HPLC method for screening potentially counterfeit anti-diabetic drugs.
abstract: Pharmaceutical counterfeiting is becoming a serious problem in the world, especially in developing countries including China. Herein an isocratic reversed-phase high performance liquid chromatography (RP-HPLC) method was developed for screening counterfeit medicines and adulterated dietary supplement products. The developed method could be employed to separate and determine simultaneously six anti-diabetic drugs (glipizide, gliclazide, glibenclamide, glimepiride, gliquidone, repaglinide) on an isocratic solvent system using an Alltima C18 column (5 microm, 150 mmx4.6 mm) with an isocratic mobile phase of methanol-phosphate buffer (pH 3.0; 0.01 mol/L) (70:30, v/v), at a flow rate of 1.0 mL/min and at a wavelength of 230 nm. The proposed method was successfully applied to the analysis of medicinal and dietary supplement samples purchased from the local market in China.
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100050, PR China. yjciae@gmail.com
authors.forename: Jing
authors.initials: J
authors.lastname: Yao
authors.forename: Ya-Qin
authors.initials: YQ
authors.lastname: Shi
authors.forename: Zhuo-Rong
authors.initials: ZR
authors.lastname: Li
authors.forename: Shao-Hong
authors.initials: SH
authors.lastname: Jin
mesh_terms: D002219:Carbamates / Q000032:analysis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005907:Gliclazide / Q000032:analysis / Q000737:chemistry; D005913:Glipizide / Q000032:analysis / Q000737:chemistry; D005905:Glyburide / Q000032:analysis / Q000737:chemistry; D007004:Hypoglycemic Agents / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D015394:Molecular Structure; D010880:Piperidines / Q000032:analysis / Q000737:chemistry; D015203:Reproducibility of Results; D013453:Sulfonylurea Compounds / Q000032:analysis / Q000737:chemistry
chemical_list: D002219:Carbamates; D007004:Hypoglycemic Agents; D010880:Piperidines; D013453:Sulfonylurea Compounds; C072379:repaglinide; C057619:glimepiride; C010969:gliquidone; D005907:Gliclazide; D005905:Glyburide; D005913:Glipizide