Contrasting impact of DNA repair gene XRCC1 polymorphisms Arg399Gln and Arg194Trp on the risk of lung cancer in the north-Indian population.
DNA repair forms the most effective defense system against DNA damage. The XRCC1 gene product is implicated in single-strand and base-excision repair mechanisms. Our main aim was to investigate the relationship between the XRCC1 gene with lung cancer on the north-Indian population. Blood samples from 225 North-Indian subjects including 103 newly diagnosed cases and 122 population-based healthy persons were collected. XRCC1 genotypes were detected using a PCR-RFLP technique. The data were analyzed by logistic regression analysis. XRCC1 polymorphisms at codon 399 were found to be protective in the development of lung cancer (OR--0.6, 95% CI--0.46-0.80, p-0.0008). The codon 194 Trp/Trp genotype was associated with a slightly increased risk of lung cancer. When assessed in nonsmokers, only the Arg/Trp genotype of XRCC1 codon 194 was positively associated with lung cancer (OR--2.3, 95% CI--0.77-7.20). Smoking also seemed to significantly interact with the combined genotypes of XRCC1 codon 399 Arg/Gln/Gln/Gln. In conclusion, the results have suggested that the XRCC1 gene might be the risk genotype for lung cancer in this population.
Pachouri
Suparna S
SS
Department of Biotechnology, Panjab University, Chandigarh, India.
Sobti
Ranbir Chander
RC
Kaur
Pushpinder
P
Singh
Jagmohan
J
D019943:Amino Acid Substitution; D001120:Arginine / Q000737:chemistry / Q000235:genetics; D016022:Case-Control Studies; D003062:Codon / Q000235:genetics; D004260:DNA Repair / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D005838:Genotype; D005973:Glutamine / Q000737:chemistry / Q000235:genetics; D006801:Humans; D007194:India; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011153:Population / Q000235:genetics; D012306:Risk; D014364:Tryptophan / Q000737:chemistry / Q000235:genetics; D000076105:X-ray Repair Cross Complementing Protein 1
D003062:Codon; D004268:DNA-Binding Proteins; D000076105:X-ray Repair Cross Complementing Protein 1; C000619371:XRCC1 protein, human; D005973:Glutamine; D014364:Tryptophan; D001120:Arginine