title: Construction of mouse adenovirus type 1 mutants.
abstract: Mouse adenovirus provides a model for studying adenovirus pathogenesis in the natural host. The ability to make viral mutants allows the investigation of specific mouse adenoviral gene contributions to virus-host interactions. Methods for propagation and titration of wild-type mouse adenovirus, production of viral DNA and viral DNA-protein complex, and transfection of mouse cells to obtain mouse adenovirus mutants are described in this chapter. Plaque purification, propagation, and titration of the mutant viruses are also presented.
authors.affiliation: Department of Natural Sciences, Clayton College and State University, Morrow, GA, USA.
authors.forename: Angela N
authors.initials: AN
authors.lastname: Cauthen
authors.forename: Amanda R
authors.initials: AR
authors.lastname: Welton
authors.forename: Katherine R
authors.initials: KR
authors.lastname: Spindler
mesh_terms: D000256:Adenoviridae / Q000235:genetics* / Q000302:isolation & purification*; D000818:Animals; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D007202:Indicators and Reagents; D051379:Mice; D009154:Mutation; D014162:Transfection / Q000379:methods; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004279:DNA, Viral; D007202:Indicators and Reagents; D014764:Viral Proteins; D004247:DNA