A tetra(L-lysine)-grafted poly(organophosphazene) for gene delivery.
In order to develop a new gene delivery vector, a novel cationic poly(organophosphazene) was synthesized by stepwise nucleophilic substitutions of poly(dichlorophosphazene) with a hydrophilic methoxy-poly(ethylene glycol) (MPEG) as a shielding group and a branched tetra(L-lysine), LysLys(LysEt)(2), as a cationic moiety. The cationic polymer has shown to form a polyplex by DNA condensation and very low in vitro cytotoxicity probably due to the shielding effect of MPEG, which provides a basis for improving the low gene transfection yield of cationic polyphosphazenes.
Jun
Yong Joo
YJ
Division of Nano Science BK 21, Ewha Womans University, 11-1 Daehyun-Dong, Seodaemun-Gu, Seoul 120-750, Republic of Korea.
Kim
Jung Hee
JH
Choi
Su Jin
SJ
Lee
Hwa Jeong
HJ
Jun
Moo Jin
MJ
Sohn
Youn Soo
YS
D045744:Cell Line, Tumor; D004247:DNA / Q000737:chemistry; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000737:chemistry*; D006801:Humans; D008156:Luciferases / Q000032:analysis / Q000235:genetics; D009943:Organophosphorus Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D011107:Polylysine / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D014162:Transfection
C521492:MPEG350; D009943:Organophosphorus Compounds; D011108:Polymers; C108974:poly(phosphazene); D011107:Polylysine; D011092:Polyethylene Glycols; D004247:DNA; C033952:tetralysine; D008156:Luciferases