title: A direct enzyme immunoassay for the measurement of furosemide in horse plasma.
abstract: A new enzyme immunoassay (EIA) for the measurement of furosemide in horse plasma is described. The lower limit of detection of this EIA method was 7.8 ng/ml. The intra-and inter-assay coefficients of variation ranged from 2.5% to 4.9% and 7.5% to 9.8%, respectively. Cross-reactivity with other compounds was not observed. There was a high correlation (r2=0.987) between the high-performance liquid chromatography and EIA results obtained for furosemide concentrations in horse plasma. These results indicate that the newly developed EIA method is useful for the quantitative analysis of furosemide in horse plasma.
authors.affiliation: Laboratory of Racing Chemistry, Japan.
authors.forename: Shun-ichi
authors.initials: S
authors.lastname: Nagata
authors.forename: Masahiko
authors.initials: M
authors.lastname: Kurosawa
authors.forename: Masao
authors.initials: M
authors.lastname: Kuwajima
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D004305:Dose-Response Relationship, Drug; D005665:Furosemide / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D006736:Horses / Q000097:blood*; D007124:Immunoenzyme Techniques / Q000379:methods* / Q000662:veterinary*; D012680:Sensitivity and Specificity
chemical_list: D005665:Furosemide