Capture ELISA quantitation of mouse adenovirus type 1 in infected organs.
A capture enzyme-linked immunosorbent assay (ELISA) was optimized for identification of mouse adenovirus type 1 in infected brain homogenates. The ELISA method allows for a much faster quantitation of virus in infected organs than plaque assays. Methods for organ homogenization and the subsequent capture ELISA are described in this chapter.
Welton
Amanda R
AR
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA.
Spindler
Katherine R
KR
D000256:Adenoviridae / Q000302:isolation & purification*; D000257:Adenoviridae Infections / Q000175:diagnosis*; D000818:Animals; D001921:Brain / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007202:Indicators and Reagents; D051379:Mice; D012449:Safety; D012680:Sensitivity and Specificity; D010948:Viral Plaque Assay
D007202:Indicators and Reagents