Effect of activated protein C on secretory activity of rat peritoneal mast cells.
Generation of thrombin and activated protein C in the inflammatory focus was demonstrated in rats with experimental acute peritonitis. The contents of thrombin and activated protein C peaked by the 30th and 120th minute of inflammation, respectively. In vitro study showed a decrease in spontaneous and compound 48/80-induced secretion of beta-hexosaminidase by peritoneal mast cells under the influence of activated protein C in low concentrations. The antiinflammatory effect of protein C in the focus of acute peritonitis is probably realized through NO release from peritoneal mast cells. This conclusion is derived from the data that L-NAME abolishes the protective effect of activated protein C.
Makarova
A M
AM
Department of Human and Animal Physiology, Biological Faculty, M. V. Lomonosov Moscow State University.
Rusanova
A V
AV
Gorbacheva
L R
LR
Umarova
B A
BA
Strukova
S M
SM
D000581:Amidohydrolases / Q000378:metabolism; D000818:Animals; D007249:Inflammation / Q000503:physiopathology; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D010529:Peritoneal Cavity; D011486:Protein C / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013917:Thrombin / Q000378:metabolism
D011486:Protein C; D013917:Thrombin; D000581:Amidohydrolases; D019331:NG-Nitroarginine Methyl Ester