Combined virus-like particle-based polyepitope DNA/protein HIV-1 vaccine design, immunogenicity and toxicity studies.
We have previously described designing of polyepitope immunogens TBI and TCI, to stimulate the humoral and cellular immune responses to HIV-1. Here, immunogens TBI and TCI were used to create new vaccine construct named CombiHIVvac (Combined HIV-1 vaccine). CombiHIVvac is a virus-like particles (VLP) containing the DNA vaccine pcDNA-TCI as a core encapsulated within a spermidine-polyglucin-TBI conjugate. The immunogenic and toxic properties of the candidate vaccine CombiHIVvac have been studied. CombiHIVvac induces a strong humoral and CTL responses in mice; the antibodies are highly specific and are able to neutralize HIV-1 in vitro. Preclinical study demonstrated that CombiHIVvac does not cause long-term changes in physiological, biochemical and morphological parameters in immunized animals and thus can be recommended for clinical trials.
Karpenko
Larisa I
LI
State Research Center of Virology and Biotechnology Vector, 630559 Koltsovo, Novosibirsk region, Russia. karpenko@vector.nsc.ru
Ilyichev
Alexander A
AA
Eroshkin
Alexey M
AM
Lebedev
Leonid R
LR
Uzhachenko
Roman V
RV
Nekrasova
Nadezhda A
NA
Plyasunova
Olga A
OA
Belavin
Pavel A
PA
Seregin
Sergei V
SV
Danilyuk
Nadezhda K
NK
Zaitsev
Boris N
BN
Danilenko
Elena D
ED
Masycheva
Valentina I
VI
Bazhan
Sergei I
SI
D016915:AIDS Vaccines / Q000009:adverse effects / Q000737:chemistry / Q000276:immunology*; D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D015483:HIV Antibodies / Q000097:blood; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D009500:Neutralization Tests; D019444:Vaccines, DNA / Q000009:adverse effects / Q000737:chemistry / Q000276:immunology*; D022721:Vaccines, Virosome / Q000009:adverse effects / Q000737:chemistry / Q000276:immunology*
D016915:AIDS Vaccines; D016207:Cytokines; D000939:Epitopes; D015483:HIV Antibodies; D019444:Vaccines, DNA; D022721:Vaccines, Virosome