Histomorphometric analysis of tissue responses to bioactive glass implants in critical defects in rat calvaria.
The aim of this study was to evaluate the osteogenic behavior of two chemically similar bioactive glass products (Biogranand Perioglas) implanted in critical bone defects in rat calvaria. Thirty-six transfixed bone defects of 8 mm diameter were made surgically in adult male Wistar rats. The animals were distributed equally into three groups: Biogran (GI), Perioglas (GII) and without implant material (control; GIII). The morphology and composition of both bioactive glasses were analyzed by scanning electron microscopy and energy-dispersive spectrometry. Tissue specimens were analyzed at the biological time points of 15, 30 and 60 days by optical microscopy and morphometry, demonstrating biocompatibility for the tested materials with moderate chronic inflammation involving their particles. Bone neoformation resulted only as a reparative reaction to an intentionally produced defect and was limited to the defect's edges. No statistically significant differences among the groups were observed. At the scar interstice, abundant deposits of collagenous fibers enveloping the particles were noted. The present results indicated that the bioactive glasses, under the experimental conditions analyzed, did not show osteogenic behavior.
Cardoso
Ana Karina M V
AK
Dentistry Faculty, Federal University of Bahia, Salvador, Brazil.
Barbosa
Aryon de Almeida
Ade A
Miguel
Fúlvio Borges
FB
Marcantonio
Elcio
E
Farina
Marcos
M
Soares
Glória Dulce de Almeida
GD
Rosa
Fabiana Paim
FP
D000818:Animals; D001843:Bone Cements; D018786:Bone Substitutes; D005898:Glass; D008297:Male; D019736:Prostheses and Implants; D051381:Rats; D017208:Rats, Wistar; D012886:Skull / Q000033:anatomy & histology* / Q000166:cytology; D013053:Spectrophotometry
C073544:Biogran; D001843:Bone Cements; D018786:Bone Substitutes