Cytokine expression in paraffin wax-embedded tissues from conventional calves.
The cross-reactivity of antibodies against human tumour necrosis factor (TNF)alpha, interleukin (IL)-1alpha, IL-1beta and porcine IL-6, and the distribution of immunolabelled cells were evaluated on paraffin wax-embedded tissues from five healthy calves. The tissues were fixed in 10% buffered formalin or Bouin's solution and processed for structural studies and immunohistochemical studies by the avidin-biotin-peroxidase technique. Bouin's solution proved to be the more suitable fixative and Tween 20 the most effective antigen unmasking technique for increasing detectable antigenicity. Constitutive expression of TNFalpha, IL-1alpha, IL-1beta and IL-6 by different cell populations, mainly macrophage-like cells, was detected. Lymphoid organs displayed a higher presence of immunolabelled cells than did lung, liver or kidney. TNFalpha and IL-1alpha appeared as the predominant cytokines, especially in the gut-associated lymphoid tissue of the ileum and in the regional mesenteric lymph nodes. The results will facilitate investigation of the role of these cytokine-producing cells in inflammatory disease processes in calves.
Pedrera
M
M
Departamento de Anatomía y Anatomía Patológica Comparadas, Facultad de Veterinaria, Universidad de Córdoba, Edificio de Sanidad Animal, Campus de Rabanales, 14014, Córdoba, Spain.
Sánchez-Cordón
P J
PJ
Romero-Trevejo
J L
JL
Raya
A I
AI
Núñez
A
A
Gómez-Villamandos
J C
JC
D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D003429:Cross Reactions; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000662:veterinary*; D008297:Male; D016612:Paraffin Embedding / Q000662:veterinary*
D000911:Antibodies, Monoclonal; D016207:Cytokines