Purification of the seven tetranortriterpenoids in neem (Azadirachta indica) seed by counter-current chromatography sequentially followed by isocratic preparative reversed-phase high-performance liquid chromatography.
Counter-current chromatography (CCC) sequentially followed by isocratic preparative reversed-phase high-performance liquid chromatography was used to isolate the seven bio-actives (azadirachtin A, azadirachtin B, azadirachtin H, desacetylnimbin, desacetylsalannin, nimbin and salannin) from the seed concentrate (NSC) of the neem tree (Azadirachta indica A. Juss). Reproducible, narrow polarity range, high purity fractions were obtained from repeated injections of the NSC (700 mg loadings/injection), on to a relatively small volume CCC coil (116 mL). The CCC biphasic solvent system chosen was hexane:butanol:methanol:water (1:0.9:1:0.9, v/v). A mass balance of injected material showed that 95+% were recovered.
Silva
Júlio César T
JC
Universidade Federal de Viçosa, Laboratório de Pesquisas de Produtos Naturais, Departamento de Química, Viçosa MG 36570-000, Brazil. juliosilva@iqm.unicamp.br
Jham
Gulab N
GN
Oliveira
Rosângela D'arc L
RD
Brown
Leslie
L
D031601:Azadirachta / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003377:Countercurrent Distribution / Q000379:methods*; D036701:Limonins / Q000737:chemistry / Q000302:isolation & purification*; D015394:Molecular Structure; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry*
D036701:Limonins