title: Negative chronotropic effect of cannabinoids and their water-soluble emulsion is related to activation of cardiac CB1 receptors.
abstract: Intravenous injection of cannabinoids dissolved in cremophore EL:ethanol:NaCl mixture and water-soluble emulsion of the same cannabinoids caused identical negative chronotropic effects in chloralose-narcotized rats. Selective CB1 and CB2 receptor antagonist HU-210 also induced a negative chronotropic effect in rats, while pre-injection of CB1 receptor antagonist SR 141716A completely abolished this effect of HU-210. Selective CB2 receptor antagonist SR 144528 had no effect on HU-210-induced bradycardia. Preinjection of ganglioblocker hexamethonium also did not abolish the negative chronotropic effect of HU-210 and ACPA. Perfusion of isolated rat heart with Krebs-Henseleit solution containing HU-210 in a final concentration of 100 nM reduced heart rate. It was shown that the negative chronotropic effect of cannabinoids is mediated through activation of cardiac CB1 receptors.
authors.affiliation: Institute of Cardiology, Siberian Division of Russian Academy of Medical Sciences, Tomsk.
authors.forename: A V
authors.initials: AV
authors.lastname: Krylatov
authors.forename: L N
authors.initials: LN
authors.lastname: Maslov
authors.forename: S Yu
authors.initials: SY
authors.lastname: Ermakov
authors.forename: E I
authors.initials: EI
authors.lastname: Barzakh
authors.forename: O V
authors.initials: OV
authors.lastname: Lasukova
authors.forename: D
authors.initials: D
authors.lastname: Crawford
authors.forename: R
authors.initials: R
authors.lastname: Ghadessy
authors.forename: V Yu
authors.initials: VY
authors.lastname: Serebrov
mesh_terms: D000818:Animals; D002186:Cannabinoids / Q000494:pharmacology*; D002698:Chloralose / Q000494:pharmacology; D013759:Dronabinol / Q000031:analogs & derivatives / Q000494:pharmacology; D004562:Electrocardiography; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D006339:Heart Rate / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D043884:Receptor, Cannabinoid, CB1 / Q000037:antagonists & inhibitors / Q000187:drug effects / Q000502:physiology*
chemical_list: D002186:Cannabinoids; D043884:Receptor, Cannabinoid, CB1; D002698:Chloralose; D013759:Dronabinol; C062018:HU 211