Measurement of Elecsys NT-proBNP in serum, K2 EDTA and heparin plasma.
OBJECTIVE
There is controversial evidence on a matrix influence on the measurement of NT-proBNP.


METHODS
We compared results of Elecsys NT-proBNP measurement on serum, K2 EDTA plasma and lithium heparin plasma.


RESULTS
Samples collected in K2 EDTA showed a marginally significant underestimation when compared to serum and heparin, whereas no significant difference was observed between serum and heparin plasma.


CONCLUSIONS
Serum, heparin and K2 EDTA plasma may be suitable for NT-proBNP measurement.
Lippi
Giuseppe
G
Sezione di Chimica e Microscopia Clinica, Dipartimento di Scienze Morfologico-Biomediche, Università degli Studi di Verona, Ospedale Policlinico G.B. Rossi, Piazzale Scuro, 10, 37134-Verona, Italy. giuseppe.lippi@univr.it
Salvagno
Gian Luca
GL
Montagnana
Martina
M
Guidi
Gian Cesare
GC
D000925:Anticoagulants; D001800:Blood Specimen Collection; D004492:Edetic Acid; D006493:Heparin; D006801:Humans; D020097:Natriuretic Peptide, Brain / Q000097:blood*; D010446:Peptide Fragments / Q000097:blood*
D000925:Anticoagulants; D010446:Peptide Fragments; C109794:pro-brain natriuretic peptide (1-76); D020097:Natriuretic Peptide, Brain; D006493:Heparin; D004492:Edetic Acid