[Cloning and characterization of down-regulated genes in neural tube defects of golden hamster induced by hyperthermia].
OBJECTIVE
To clone down-regulated genes in neural tube defects of golden hamster induced by hyperthermia and to explore the molecular mechanism.


METHODS
A reversed subtractive cDNA library was constructed using suppression subtractive hybridization. Clones with inserts were selected by combination of alpha complementary phenomenon and colony PCR. Then sequence and homology analysis of the inserts were made. And mRNA expression conditions were confirmed by Northern blot.


RESULTS
The reversed subtractive cDNA library was successfully constructed. Fifteen recombinant clones were sequenced and recognized homologous to known genes. The results of Northern blot showed that all these genes were down-regulated in defected neural tubes induced by hyperthermia compared to normal developed neural tubes.


CONCLUSIONS
Some important genes are identified which might be involved in neural tube defects induced by hyperthermia.
Yang
Qing
Q
Histology and Embryology Institution, Medical School of Shandong University, Jinan, Shandong, 250012 PR China.
Gao
Ying-mao
YM
Li
Shao-ling
SL
D000818:Animals; D015152:Blotting, Northern; D003001:Cloning, Molecular; D006224:Cricetinae; D005260:Female; D015723:Gene Library; D006979:Hyperthermia, Induced / Q000009:adverse effects*; D008647:Mesocricetus; D009436:Neural Tube Defects / Q000209:etiology / Q000235:genetics*