Regioselective and enzymatic production of gamma-resorcylic acid from resorcinol using recombinant Escherichia coli cells expressing a novel decarboxylase gene.
A recombinant Escherichia coli, expressing the rdc gene, which encodes a gamma-resorcylic acid decarboxylase (Rdc) reversibly catalyzing regioselective carboxylation of resorcinol derived from Rhizobium radiobacter WU-0108, converted 20 mM resorcinol to gamma-resorcylic acid with a 44% (mol/mol) yield at 30 degrees C for 7 h. The recombinant E. coli cells were recyclable at least five times for use as biocatalysts.
Iwasaki
Yuichiro
Y
Department of Applied Chemistry, School of Science and Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku-ku, Tokyo, 169-8555, Japan.
Kino
Kuniki
K
Nishide
Hiroyuki
H
Kirimura
Kohtaro
K
D016960:Agrobacterium tumefaciens / Q000201:enzymology*; D002262:Carboxy-Lyases / Q000235:genetics*; D002384:Catalysis; D004926:Escherichia coli / Q000166:cytology* / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects; D062385:Hydroxybenzoates / Q000737:chemistry / Q000378:metabolism*; D012118:Resorcinols / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D013237:Stereoisomerism
D062385:Hydroxybenzoates; D012118:Resorcinols; D002262:Carboxy-Lyases; C493926:gamma-resorcylic acid decarboxylase, Rhizobium radiobacter; C412605:gamma-resorcylic acid; C031389:resorcinol