title: Western blotting.
abstract: Western blotting is one of the most commonly used biochemical techniques to detect a specific protein from a mixture of proteins such as tissue extracts. Antibodies to the specific antigen are used to detect the protein. The mixture of proteins is resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to a membrane. A specific antigen immobilized on the membrane is detected and visualized by a primary antibody, a secondary antibody-peroxidase conjugate, and a chemiluminescent reagent.
authors.affiliation: Department of Biomedical Sciences, Florida State University College of Medicine, Tallahassee, USA.
authors.forename: Choogon
authors.initials: C
authors.lastname: Lee
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods*; D056926:CLOCK Proteins; D002940:Circadian Rhythm; D004591:Electrophoresis, Polyacrylamide Gel; D008163:Luminescent Measurements; D011506:Proteins / Q000302:isolation & purification; D015534:Trans-Activators / Q000302:isolation & purification
chemical_list: D011506:Proteins; D015534:Trans-Activators; D056926:CLOCK Proteins