title: Baculoviral vector-mediated transient and stable transgene expression in human embryonic stem cells.
abstract: Human embryonic stem (hES) cells as a renewable cell source have great prospective applications in both developmental biology research and regenerative medicine. To realize these potentials, the development of effective and safe genetic manipulation methods in hES cells is an obvious demand. We report here that baculoviral vectors were able to transduce hES cells efficiently. In transient transduction experiments, a recombinant baculoviral vector equipped with a human elongation factor 1-alpha promoter and a woodchuck hepatitis post-transcriptional regulatory element transduced up to 80% of cells in hES cell clumps and embryoid bodies. For prolonged transgene expression, hybrid baculoviral vectors that have incorporated a rep gene and inverted terminal repeat sequences from adeno-associated virus were produced. These hybrid vectors yielded stable transgene expression during the prolonged undifferentiated proliferation of hES cells and after differentiation. Baculoviral transduction did not affect the normal growth, phenotype, and pluripotency of hES cells. Thus, baculoviral vectors suitable for both transient overexpression and long-term stable expression are an attractive option for genetic manipulation of hES cells.
authors.affiliation: Institute of Bioengineering and Nanotechnology, Singapore.
authors.forename: Jieming
authors.initials: J
authors.lastname: Zeng
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhao
authors.forename: Nallasivam
authors.initials: N
authors.lastname: Palanisamy
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
mesh_terms: D016367:Baculoviridae / Q000235:genetics*; D053595:Embryonic Stem Cells / Q000502:physiology* / Q000637:transplantation* / Q000821:virology; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D005822:Genetic Vectors; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics; D007150:Immunohistochemistry; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014161:Transduction, Genetic; D014162:Transfection; D019076:Transgenes
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins