title: Quantitative microcomputed tomography assessment of intratrabecular, intertrabecular, and cortical bone architecture in a rat model of severe renal osteodystrophy.
abstract: OBJECTIVE
  To determine the effects of renal osteodystrophy (ROD) on bone microarchitecture in growing rats.
  
  
  METHODS
  A total of 24 rats underwent 5/6 nephrectomy (NX) and were fed a high-phosphorus diet to induce ROD; another 6 underwent sham NX. In vitro microcomputed tomography images (GEMS, London, Ontario, Canada) were obtained in the femoral metaphysis and midshaft.
  
  
  RESULTS
  Trabecular and cortical bone volume/total volume (BV/TV) were significantly lower in NX specimens because of pores within the trabeculae and along the endosteal surface. Topological analysis using component labeling in 3-dimensions verified that trabecular pores connected to the marrow space. After the trabecular pores were filled using a morphological filter, trabecular thickness was significantly increased in NX. In contrast, cortical thickness was significantly decreased in NX compared with controls; however, after filling the endocortical pores, thickness did not differ.
  
  
  CONCLUSIONS
  The ROD resulted in decreased cortical and trabecular BV/TV, increased porosity, and increased trabecular thickness. Advanced image processing algorithms demonstrated the effects of cortical and trabecular porosity on BV/TV and structure in ROD.
authors.affiliation: School of Physical and Chemical Sciences, Queensland University of Technology, Brisbane, Australia.
authors.forename: Tim A J
authors.initials: TA
authors.lastname: Hopper
authors.forename: Felix W
authors.initials: FW
authors.lastname: Wehrli
authors.forename: Punam K
authors.initials: PK
authors.lastname: Saha
authors.forename: Jalal B
authors.initials: JB
authors.lastname: Andre
authors.forename: Alexander C
authors.initials: AC
authors.lastname: Wright
authors.forename: Cheryl P
authors.initials: CP
authors.lastname: Sanchez
authors.forename: Mary B
authors.initials: MB
authors.lastname: Leonard
mesh_terms: D000818:Animals; D001837:Body Weights and Measures / Q000379:methods; D050071:Bone Density Conservation Agents / Q000008:administration & dosage; D002117:Calcitriol / Q000008:administration & dosage; D012080:Chronic Kidney Disease-Mineral and Bone Disorder / Q000175:diagnosis* / Q000000981:diagnostic imaging / Q000188:drug therapy; D004195:Disease Models, Animal; D005269:Femur / Q000000981:diagnostic imaging*; D013006:Growth Hormone / Q000008:administration & dosage; D021621:Imaging, Three-Dimensional / Q000379:methods; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012720:Severity of Illness Index; D014057:Tomography, X-Ray Computed / Q000379:methods*
chemical_list: D050071:Bone Density Conservation Agents; D013006:Growth Hormone; D002117:Calcitriol