Cytotoxic activity of a dichloromethane extract and fractions obtained from Eudistoma vannamei (Tunicata: Ascidiacea).
This study consists of the bioassay-guided fractionation of the dichloromethane extract from Eudistoma vannamei and the pharmacological characterization of the active fractions. The dried hydromethanolic extract dissolved in aqueous methanol was partitioned with dichloromethane and chromatographed on a silica gel flash column. The anti-proliferative effect was monitored by the MTT assay. Four of the latest fractions, numbered 14 to 17, which held many chemical similarities amongst each other, were found to be the most active. The selected fractions were tested for viability, proliferation and death induction on cultures of HL-60 promyeloblastic leukemia cells. The results suggested that the observed cytotoxicity is related to apoptosis induction.
Jimenez
Paula C
PC
Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, P.O. Box-3157, 60430-270, Fortaleza, Ceará, Brazil.
Wilke
Diego V
DV
Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, P.O. Box-3157, 60430-270, Fortaleza, Ceará, Brazil.
Takeara
Renata
R
Departamento de Física e Química, Faculdade de Ciências, Filosofia e Letras de Ribeirão Preto, Universidade de São Paulo, Av. do Café s/n, 14040-903, Ribeirão Preto, São Paulo, Brazil.
Lotufo
Tito M C
TMC
Instituto de Ciências do Mar, Universidade Federal do Ceará, Av. Abolição 3207, 60165-082, Fortaleza, Ceará, Brazil.
Pessoa
Cláudia
C
Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, P.O. Box-3157, 60430-270, Fortaleza, Ceará, Brazil.
Odorico de Moraes
Manoel
M
Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, P.O. Box-3157, 60430-270, Fortaleza, Ceará, Brazil.
Lopes
Norberto P
NP
Departamento de Física e Química, Faculdade de Ciências, Filosofia e Letras de Ribeirão Preto, Universidade de São Paulo, Av. do Café s/n, 14040-903, Ribeirão Preto, São Paulo, Brazil. Electronic address: npelopes@fcfrp.usp.br.
Costa-Lotufo
Letícia V
LV
Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, P.O. Box-3157, 60430-270, Fortaleza, Ceará, Brazil. Electronic address: lvcosta@secrel.com.br.
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002855:Chromatography, Thin Layer; D054697:Cytostatic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D003603:Cytotoxins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis; D018922:HL-60 Cells; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000188:drug therapy* / Q000473:pathology; D008752:Methylene Chloride; D019906:Nuclear Magnetic Resonance, Biomolecular; D014561:Urochordata / Q000737:chemistry*
D054697:Cytostatic Agents; D003603:Cytotoxins; D008752:Methylene Chloride; D004247:DNA