Persistence of hyperbaric oxygen-induced oxidative effects after exposure in rat brain cortex tissue.
Hyperbaric oxygen (HBO) causes oxidative stress in several organs and tissues. Due to its high rate of blood flow and oxygen consumption, the brain is one of the most sensitive organs to this effect. Many studies have reported oxidative effects of HBO, but there is no comprehensive data about how long this effect persists. The aim of this study was to elucidate the duration of HBO-induced oxidative/antioxidant action. Male Sprague-Dawley rats were divided into 5 groups. Except for the controls, the animals were subjected to 100% oxygen for 2 h at 3 atm and differed from each other by the time to dissection after exposure that began at 30, 60, 90, or 120 min. Thiobarbituric acid-reactive substances (TBARS), as well as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity was determined in brain cortex tissue. Additionally, nitrite-nitrate (NO(x)) concentrations were measured. All measured parameters were found to be significantly increased 30 min after exposure. SOD and GSH-Px levels persisted at significantly high levels for 60 min. In conclusion, the oxidative effect of HBO was shown to persist only for 1 h. Further studies should be performed to elucidate the possible molecular interactions during this period.
Ay
Hakan
H
Department of Undersea and Hyperbaric Medicine, Gülhane Military Medical Academy, 06018 - Etlik/Ankara, Turkey.
Topal
Turgut
T
Ozler
Mehmet
M
Uysal
Bülent
B
Korkmaz
Ahmet
A
Oter
Sükrü
S
Ogur
Recai
R
Dündar
Kadir
K
D000280:Administration, Inhalation; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects* / Q000201:enzymology*; D004334:Drug Administration Schedule; D005979:Glutathione Peroxidase / Q000378:metabolism*; D006931:Hyperbaric Oxygenation; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress; D010100:Oxygen / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000032:analysis; D013482:Superoxide Dismutase / Q000032:analysis*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D010100:Oxygen