title: [Loss of heterozygosity in the region of chromosome 4p15.1-4q12 in nasopharyngeal carcinoma patients with familial history].
abstract: OBJECTIVE
  To analyze the allelic loss of heterozygosity (LOH) in the region of chromosome 4p15.1-4q12 in nasopharyngeal carcinoma (NPC) patients with family history.
  
  
  METHODS
  Tumor cells and lymphocytes were obtained from paraffin-embedded biopsied tissue section by microdissection. LOH detections were carried out on 25 NPC patients with family history by PCR-based microsatellite polymorphism analysis using 7 pairs of microsatellite markers primers. The microsatellite loci located in 4p15.1-4q12 region. Genescan software was used to analyse LOH at each locus.
  
  
  RESULTS
  Ninety-two percent of NPC cases (23/25) with family history was showed at least one microsatellite marker of LOH. Higher frequencies of LOH were found at three loci: D4S238 (56%), D4S350 (50%), D4S1547 (50%). The minimal common region of deletion might be defined between D4S350 and D4S1547.
  
  
  CONCLUSIONS
  The higher incidence of LOH at D4S350 and D4S1547 suggests that there may be a potential tumor suppressor gene located in the two regions.
authors.affiliation: Experimental Medical Research Center, Guangzhou Medical College, Guangzhou, Guangdong, 510182 PR China.
authors.forename: Qi-cai
authors.initials: QC
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Fang
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Li
authors.forename: Qi-shen
authors.initials: QS
authors.lastname: Feng
authors.forename: Li-xi
authors.initials: LX
authors.lastname: Huang
authors.forename: Yi-xin
authors.initials: YX
authors.lastname: Zeng
mesh_terms: D000328:Adult; D002894:Chromosomes, Human, Pair 4 / Q000235:genetics*; D005192:Family Health; D005260:Female; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction