Regulation of MMP-1 by sex steroid hormones in fibroblasts derived from the female pelvic floor.
OBJECTIVE
The aim of this study was to investigate the effect of sex steroid hormones on the overall expression profile of cellular matrix metalloproteinase-1 (MMP-1) in fibroblasts that are derived from arcus tendineus fasciae pelvis.


METHODS
Arcus tendineus fasciae pelvis fibroblasts that originated from a premenopausal woman and a postmenopausal woman who was undergoing a prolapse repair were treated with physiologic concentrations of 17-beta-estradiol (E2), progesterone, E2 plus progesterone, and E2 plus ICI 182,780. Cellular expressions of the latent, active, and fragment forms of MMP-1 were analyzed quantitatively by Western immunoblotting.


RESULTS
The latent and fragment forms of MMP-1 were increased by E2, progesterone, and E2 plus progesterone. The active form of MMP-1 was not changed by either E2 or progesterone alone but was decreased significantly when both hormones were added together. ICI 182,780 inhibited the stimulatory effect of E2.


CONCLUSIONS
Fragmentation is a site of regulation of MMP-1 expression by hormones. Only E2 combined with progesterone decreased the active form of MMP-1, which suggests that both hormones are necessary to maintain the integrity of female pelvic floor.
Zong
Wenjun
W
Division of Urogynecology and Reconstructive Pelvic Surgery, Department of Obstetrics and Gynecology and Reproductive Sciences, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Zyczynski
Halina M
HM
Meyn
Leslie A
LA
Gordy
Susan C
SC
Moalli
Pamela A
PA
D000328:Adult; D000368:Aged; D015153:Blotting, Western; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology*; D005455:Fluorescent Antibody Technique; D012739:Gonadal Steroid Hormones / Q000494:pharmacology*; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000187:drug effects* / Q000378:metabolism*; D008875:Middle Aged; D017773:Pelvic Floor; D017698:Postmenopause; D017697:Premenopause; D011336:Probability; D012494:Sampling Studies; D012680:Sensitivity and Specificity
D012739:Gonadal Steroid Hormones; D020781:Matrix Metalloproteinase 1