Genotyping of Chlamydia trachomatis from endocervical specimens in Brazil.
BACKGROUND
There is no data concerning genotyping of Chlamydia trachomatis from Brazilian samples.


OBJECTIVE
To characterize the genotype of C. trachomatis detected in women assisted at a STD public clinic and establish the prevalence of this infection in that population.


METHODS
Endocervical samples of a group of 100 women were tested for chlamydial infection with PCR directed to C. trachomatis cryptic plasmid. Genotyping of positive samples were done after omp1 amplification and sequencing.


RESULTS
The overall prevalence of C. trachomatis infection was 19%, with the highest prevalence in women between 15 and 25 years old (68.4%). Four genotypes were found associated with endocervical infections: D, E, F, and K. Sequence analysis revealed a coinfection of genotypes D and E in 1 woman.


CONCLUSIONS
To our knowledge this is the first study to characterize Brazilian C. trachomatis endocervical samples and Brazilian C. trachomatis genotype coinfection. Our results also emphasize the importance of routine diagnosis of C. trachomatis for the control of this STD.
Lima
Haleta E
HE
Departamento de Microbiologia, Universidade Federal de Minas Gerais, Brasil.
Oliveira
Milena B
MB
Valente
Brenda G
BG
Afonso
Daniela A F
DA
Darocha
Wanderson D
WD
Souza
Maria Carmo M
MC
Alvim
Túlio C
TC
Barbosa-Stancioli
Edel F
EF
Noronha
Fátima Soares Motta
FS
D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D001938:Brazil / Q000453:epidemiology; D002690:Chlamydia Infections / Q000453:epidemiology* / Q000382:microbiology* / Q000517:prevention & control; D002692:Chlamydia trachomatis / Q000235:genetics* / Q000302:isolation & purification; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015995:Prevalence; D014626:Vaginal Smears
D017931:DNA Primers; D004269:DNA, Bacterial