title: Characterization of an HIV-1 group M variant that is distinct from the known subtypes.
abstract: We identified an HIV-1 variant that belongs to the M group, with limited similarity of short genetic regions (100-200 nt) to subtype K, but the remainder of the genome is unrelated to any established HIV-1 subtype. The isolate was obtained from an HIV-1-positive male, living in the Netherlands, who encountered the virus before 1989, most probably via heterosexual contact in Africa. We describe the full-length genome sequence of four biological clones that were obtained from two samples collected 5 years apart. At both time points all open reading frames were intact. Within the 5-year interval, the person received antiretroviral therapy with zalcitabine and zidovudine for almost 4 years. Evolution of drug-resistant variants is likely given the increase in viral RNA load to +/-10,000 copies/ml during the last year of treatment. Surprisingly, the only regular RT mutation acquired during this period was K70R, which suggests that the genetic background of this variant is perhaps not suitable for the generation of the standard 41L, 67N, and 215Y/F mutations that typically arise during prolonged, nonsuccessful, zidovudine treatment. Awaiting the discovery of at least two additional, epidemiologically unrelated patients with a phylogenetically related HIV-1 variant, we can designate this variant a new HIV-1 subtype, or a distinct branch of subtype K.
authors.affiliation: Laboratory of Experimental Virology, and Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, Amsterdam, the Netherlands. c.m.vanderhoek@amc.uva.nl
authors.forename: Lia
authors.initials: L
authors.lastname: van der Hoek
authors.forename: Georgios
authors.initials: G
authors.lastname: Pollakis
authors.forename: Vladimir V
authors.initials: VV
authors.lastname: Lukashov
authors.forename: Maarten F
authors.initials: MF
authors.lastname: Jebbink
authors.forename: Rienk E
authors.initials: RE
authors.lastname: Jeeninga
authors.forename: Margreet
authors.initials: M
authors.lastname: Bakker
authors.forename: Nicole
authors.initials: N
authors.lastname: Dukers
authors.forename: Suzanne
authors.initials: S
authors.lastname: Jurriaans
authors.forename: William A
authors.initials: WA
authors.lastname: Paxton
authors.forename: Nicole K T
authors.initials: NK
authors.lastname: Back
authors.forename: Ben
authors.initials: B
authors.lastname: Berkhout
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012194:RNA-Directed DNA Polymerase / Q000737:chemistry*; D020539:Sequence Analysis, Protein
chemical_list: D012194:RNA-Directed DNA Polymerase