Influence of disk preparation on detection of metallo-beta-lactamase-producing isolates by the combined disk assay.
The combined disk assay has been used for detection of metallo-beta-lactamase-producing isolates. We have observed that the size of inhibition zones produced by many beta-lactam/metallo-beta-lactamase inhibitor (IMBL) combinations may differ depending on the way that the combined disks were prepared. Among the 10 beta-lactam/IMBL combinations tested, only the imipenem/EDTA combination produced similar results.
Andrade
Soraya S
SS
Laboratório Especial de Microbiologia Clínica, Universidade Federal de São Paulo, São Paulo, Brazil. soraya.andrade@lemc.com.br
Picão
Renata C
RC
Campana
Eloiza H
EH
Nicoletti
Adriana G
AG
Pignatari
Antônio C C
AC
Gales
Ana C
AC
D000362:Agar; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015780:Carbapenems / Q000494:pharmacology; D002442:Ceftazidime / Q000494:pharmacology*; D003470:Culture Media; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000201:enzymology / Q000302:isolation & purification; D006801:Humans; D015378:Imipenem / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000295:instrumentation* / Q000379:methods; D018440:beta-Lactam Resistance; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases / Q000096:biosynthesis*
D000900:Anti-Bacterial Agents; D015780:Carbapenems; D003470:Culture Media; D065093:beta-Lactamase Inhibitors; D015378:Imipenem; D000362:Agar; D002442:Ceftazidime; D001618:beta-Lactamases