A phosphate starvation-induced acid phosphatase from Oryza sativa: phosphate regulation and transgenic expression.
A phosphate starvation-induced acid phosphatase cDNA was cloned from the rice, Oryza sativa. The cDNA encoding O. sativa acid phosphatase (OsACP1) has 1100 bp with an open reading frame of 274 amino acid residues. The deduced amino acid sequence of OsACP1 cDNA showed 53% identity to tomato acid phosphatase and 46-50% identity to several other plant phosphatases. OsACP1 expression was up-regulated in the rice plant and in cell culture in the absence of phosphate (Pi). The induced expression of OsACP1 was a specific response to Pi starvation, and was not affected by the deprivation of other nutrients. OsACP1 expression was responsive to the level of Pi supply, with transcripts of OsACP1 being abundant in Pi-deprived root. The OsACP1 cDNA was expressed as a 30 kDa polypeptide in baculovirus-infected insect Sf9 cells. In addition, the OsACP1 gene was introduced into Arabidopsis via Agrobacterium-mediated transformation. Functional expression of the OsACP1 gene in the transgenic Arabidopsis lines was confirmed by Northern blot and Western blot analyses, as well as phosphatase activity assays. These results suggest that the OsACP1 gene can be used to develop new transgenic dicotyledonous plants able to adapt to Pi-deficient conditions.
Hur
Yeon Jae
YJ
College of Natural Resources and Life Science, Dong-A University, Busan, 604-714, Korea.
Lee
Han Gil
HG
Jeon
Eun Ji
EJ
Lee
Yun Young
YY
Nam
Min Hee
MH
Yi
Gihwan
G
Eun
Moo Young
MY
Nam
Jaesung
J
Lee
Jai Heon
JH
Kim
Doh Hoon
DH
D000135:Acid Phosphatase / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000235:genetics; D016367:Baculoviridae; D004790:Enzyme Induction; D018506:Gene Expression Regulation, Plant; D007313:Insecta / Q000821:virology; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology* / Q000235:genetics; D010710:Phosphates / Q000172:deficiency* / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins / Q000378:metabolism
D010710:Phosphates; D011994:Recombinant Proteins; D000135:Acid Phosphatase