title: Immunohistochemistry in Drosophila: sections and whole mounts.
abstract: This chapter describes immunohistochemistry on Drosophila heads (brains) with respect to antigens involved in the circadian system. Two different methods have been successfully performed in several labs: immunolabeling on whole-mount brains and on cryostat sections of entire heads. Both methods are addressed here. The primary antisera can be detected by enzyme-labeled or fluorescence-labeled secondary antibodies. The advantages of the different methods are discussed.
authors.affiliation: Institut für Zoologie, Universität Regensburg, Germany.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Helfrich-Förster
mesh_terms: D000818:Animals; D000906:Antibodies; D001921:Brain / Q000276:immunology / Q000502:physiology; D002940:Circadian Rhythm / Q000502:physiology*; D016616:Cryoultramicrotomy; D004210:Dissection; D004330:Drosophila / Q000276:immunology / Q000502:physiology*; D005456:Fluorescent Dyes; D007150:Immunohistochemistry / Q000379:methods*; D013194:Staining and Labeling; D016707:Tissue Fixation
chemical_list: D000906:Antibodies; D005456:Fluorescent Dyes