Use of a commercially available reagent for the selective detection of homocysteine in plasma.
A procedure for the detection of homocysteine (Hcy) in blood plasma is described. A commercially available chromogen is added to the plasma sample. The plasma solution turns from yellow to blue upon heating for 4 min when a detectable threshold level of Hcy is present. Chromatographic separations and immunogenic materials are not needed. The protocol takes approximately 30 min.
Escobedo
Jorge O
JO
Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana 70803, USA.
Wang
Weihua
W
Strongin
Robert M
RM
D002863:Chromogenic Compounds; D006710:Homocysteine / Q000097:blood*; D006801:Humans
D002863:Chromogenic Compounds; D006710:Homocysteine