A rapid method for chloroplast isolation from the green alga Chlamydomonas reinhardtii.
This method has been developed to yield highly purified intact chloroplasts from Chlamydomonas reinhardtii. This procedure involves breaking cell-wall-deficient cells by passage through a narrow-bore syringe needle and purifying the intact chloroplasts by differential centrifugation and Percoll gradient centrifugation. This procedure can be completed in less than 3 h and is capable of generating relatively high yields of chloroplasts that should be useful for researchers studying the biochemistry and cell biology of C. reinhardtii chloroplasts.
Mason
Catherine B
CB
Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana 70803, USA.
Bricker
Terry M
TM
Moroney
James V
JV
D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000201:enzymology / Q000648:ultrastructure; D002736:Chloroplasts / Q000201:enzymology / Q000648:ultrastructure; D009339:Needles