A protein microarray prepared with phage-displayed antibody clones.
Using a large phage antibody library, a protein microarray spotted directly with phage-displayed antibody clones was created to discriminate between recognition profiles of samples from healthy donors and leukemia patients. The protocol for preparing antibody-displaying phage chips was presented. Some conditions such as substrates and blocking buffers were compared and optimized. The major improvements of this microarray are higher throughput and lower cost compared to previous antibody chips. Due to its convenience and sensitivity, it can be extensively used for rapid and high throughput detection of protein profiles of experimental and clinical samples.
Bi
Qun
Q
College of Life Sciences, Peking University, Beijing 100871, PR China.
Cen
Xiaodong
X
Wang
Wenjuan
W
Zhao
Xinsheng
X
Wang
Xuan
X
Shen
Ti
T
Zhu
Shenggeng
S
D000447:Aldehydes / Q000737:chemistry; D000906:Antibodies / Q000737:chemistry*; D001798:Blood Proteins / Q000032:analysis; D004852:Epoxy Compounds / Q000737:chemistry; D005453:Fluorescence; D006801:Humans; D007962:Leukocytes / Q000737:chemistry; D019151:Peptide Library; D040081:Protein Array Analysis / Q000379:methods*; D012680:Sensitivity and Specificity
D000447:Aldehydes; D000906:Antibodies; D001798:Blood Proteins; D004852:Epoxy Compounds; D019151:Peptide Library