From the one-bead-one-compound concept to one-bead-one-reactor.
The one-bead-one-compound method gives access to millions of compounds that can be screened directly on the bead. Although characterization techniques are increasingly potent and reliable, problems can still be encountered in deciphering the sequence of the active compound because of sensitiveness or manipulation of the bead. ChemMatrix, a totally PEG-based resin, has resolved the synthesis of peptides of outstanding difficulty. Like other PEG-based resins, it permits on-bead screening because of its compatibility in aqueous media and has the further advantage of having a high loading, comparable to polystyrene resins. ChemMatrix beads previously swelled in water can be nicely divided into four parts that can be characterized using different analytical techniques or just stored for safety or for further testing. The four bead parts show high homogeneity and can thus be considered to be replicas.
Marani
Mariela M
MM
Institute for Research in Biomedicine, Barcelona Science Park, Josep Samitier 1-5, 08028-Barcelona, Spain.
Paradís-Bas
Marta
M
Tulla-Puche
Judit
J
Côté
Simón
S
Camperi
Silvia A
SA
Cascone
Osvaldo
O
Albericio
Fernando
F
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D020650:Combinatorial Chemistry Techniques / Q000379:methods; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D012117:Resins, Synthetic / Q000737:chemistry*; D012680:Sensitivity and Specificity; D014867:Water / Q000737:chemistry
D010455:Peptides; D012117:Resins, Synthetic; D014867:Water; D011092:Polyethylene Glycols