title: Evidence of natural recombination in classical swine fever virus.
abstract: Classical swine fever (CSF) virus, one member of the family Flaviviridae is the pathogen of CSF, an economically important and highly contagious disease of pigs. Although homologous recombination has been demonstrated in many other members of the family, it is unknown whether there is recombination in natural populations of CSFV. To detect possible recombination events, we performed a phylogenetic analysis of 25 full-length CSFV strains isolated all over the world. Putative recombinant sequences were identified with the use of SimPlot program. Recombination events were confirmed by bootscaning. A mosaic virus, CSFV 39 (AF407339) isolated in China was found. And its two putative parental-like strains CSFV Shimen (AF333000) and GXWZ02 (AY367767) were identified. Our work revealed that homologous recombination occurred in natural CSFV populations, generating genetic diversity. This would provide some insights for the role homologous recombinant plays in CSFV evolution.
authors.affiliation: College of Life Science, Shandong Normal University, Jinan 250014, China. hchqiang@yahoo.com.cn
authors.forename: Cheng-Qiang
authors.initials: CQ
authors.lastname: He
authors.forename: Nai-Zheng
authors.initials: NZ
authors.lastname: Ding
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Chen
authors.forename: Yun-Long
authors.initials: YL
authors.lastname: Li
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China; D006691:Classical Swine Fever / Q000821:virology; D006692:Classical Swine Fever Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009030:Mosaicism; D010802:Phylogeny; D011995:Recombination, Genetic; D012689:Sequence Homology, Nucleic Acid; D013552:Swine
chemical_list: D004279:DNA, Viral