title: N-acetylchitooligosaccharide is a potent angiogenic inhibitor both in vivo and in vitro.
abstract: N-acetylchitooligosaccharide (N-acetyl-COs) was prepared by N-acetylation of chitooligosaccharide (COs). In vitro study using human umbilical vein endothelial cells (HUVECs) revealed that both N-acetyl-COs and COs inhibited the proliferation of HUVECs by inducing apoptosis. Treatment of HUVECs by N-acetyl-COs resulted in a significant reduction of density of the migration cells and repressed tubulogenesis process. The antiangiogenic effects of the oligosaccharides were further evaluated using in vivo zebrafish angiogenesis model, and the results showed that both oligosaccharides inhibited the growth of subintestinal vessels (SIV) of zebrafish embryos in a dose-dependent manner, as observed by endogenous alkaline phosphatase (EAP) staining assay. In contrast, no cytotoxicity was found when treating the NIH3T3 and several other cancer cells with the oligosaccharides. Our results also confirmed the antiangiogenic activity of N-acetyl-COs was significantly stronger than the parent oligosaccharide, COs.
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wang
authors.forename: Lanhong
authors.initials: L
authors.lastname: Zheng
authors.forename: Shaoli
authors.initials: S
authors.lastname: Yang
authors.forename: Rongli
authors.initials: R
authors.lastname: Niu
authors.forename: Edward
authors.initials: E
authors.lastname: Chu
authors.forename: Xiukun
authors.initials: X
authors.lastname: Lin
mesh_terms: D020533:Angiogenesis Inhibitors / Q000008:administration & dosage*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D051379:Mice; D008833:Microcirculation / Q000166:cytology* / Q000187:drug effects* / Q000196:embryology; D041681:NIH 3T3 Cells; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D009844:Oligosaccharides / Q000008:administration & dosage*; D015027:Zebrafish
chemical_list: D020533:Angiogenesis Inhibitors; C520160:N-acetylchitooligosaccharide; D009844:Oligosaccharides