Activity-based fingerprinting and inhibitor discovery of cysteine proteases in a microarray.
A panel of 20 peptide vinyl sulfone probes has been synthesized and used to generate activity-based fingerprinting profiles of cysteine proteases in both gel- and microarray-based formats; the inhibitor fingerprints of representative small molecule inhibitors targeted against 4 cysteine proteases were also obtained, in high-throughput, using the same protein microarray platform.
Uttamchandani
Mahesh
M
Department of Biological Sciences, National University of Singapore, 4 Science Drive 4, 117543, Singapore.
Liu
Kai
K
Panicker
Resmi C
RC
Yao
Shao Q
SQ
D003546:Cysteine Endopeptidases / Q000032:analysis / Q000187:drug effects*; D015853:Cysteine Proteinase Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D015335:Molecular Probes / Q000138:chemical synthesis / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry; D040081:Protein Array Analysis / Q000379:methods*; D013450:Sulfones / Q000737:chemistry
D015853:Cysteine Proteinase Inhibitors; D015335:Molecular Probes; D010455:Peptides; D013450:Sulfones; C009873:divinyl sulfone; D003546:Cysteine Endopeptidases