Study of optical parameters of the nucleoli under the effect of transcription inhibitors by coherent phase microscopy.
The use of coherent phase microscopy for online quantitative registration of nucleolar reaction to transcription inhibition is validated. Reduction of phase thickness of the nucleoli was detected during the first minutes of the experiment; 30 min after addition of the drug rarefaction zones predominated and areas of condensation were seen. These changes reflect the dynamics of disorders in the nucleolar ultrastructure during transcription inhibition.
Tychinskii
V P
VP
Moscow State Institute of Radioengineering, Electronics, and Auto-matic Machines. vtych@yandex.ru
Kretushev
A V
AV
Klemyashov
I V
IV
Vyshenskaya
T V
TV
Filippova
N A
NA
Raikhlin
N T
NT
Shtil'
A A
AA
D016475:3T3 Cells; D000818:Animals; D001943:Breast Neoplasms; D045744:Cell Line, Tumor; D002466:Cell Nucleolus / Q000187:drug effects / Q000473:pathology* / Q000648:ultrastructure; D003609:Dactinomycin / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007700:Kinetics; D051379:Mice; D012004:Rectal Neoplasms; D014158:Transcription, Genetic / Q000187:drug effects*
D003609:Dactinomycin