Resistance of monocyte to HIV-1 infection is not due to uncoating defect.
Freshly isolated blood monocytes show an early postentry block to in vitro HIV-1 infection. Differentiation into monocyte-derived macrophages (MDMs) is required to allow HIV replication in this cell type. In this study, we investigated whether the resistance of monocyte to HIV infection stemmed from uncoating defect. Monocyte and MDM lysates induced HIV-1 core uncoating to a comparable degree. This suggests that monocyte lacks a factor(s) essential for viral reverse transcription and/or contains a factor(s) interfering with this step.
Srichatrapimuk
Sirawat
S
Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
Auewarakul
Prasert
P
D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D015497:HIV-1 / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D008264:Macrophages / Q000821:virology; D009000:Monocytes / Q000821:virology*; D048348:Reverse Transcription; D053586:Virus Internalization; D014779:Virus Replication
D017931:DNA Primers; D004279:DNA, Viral