Extraction of plant RNA.
This protocol details an RNA preparation for medium-scale, high-purity RNA production from higher plants. It uses hot acid phenol with standard sodium acetate ethanol precipitation and is suitable for producing RNA for both Northern blotting and enzyme-based downstream applications such as RT-PCR and microarray studies.
Salter
Michael G
MG
Department of Biology, University of Leicester, UK.
Conlon
Helen E
HE
D017360:Arabidopsis / Q000737:chemistry / Q000235:genetics; D015152:Blotting, Northern; D011232:Chemical Precipitation; D000431:Ethanol; D020411:Oligonucleotide Array Sequence Analysis; D019800:Phenol; D018749:RNA, Plant / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019346:Sodium Acetate
D018749:RNA, Plant; D019800:Phenol; D000431:Ethanol; D019346:Sodium Acetate