[Effect of low serum culture on the synchrony of cell cycle of mesenchymal stem cells].
OBJECTIVE
To investigate a suitable method of inducing cell cycle synchronization at G0/G1 stage of mesenchymal stem cells (MSCs).


METHODS
MSCs were cultured and identified with CD44, CD90, CD71 and CD11b by flow cytometer. Cell cycle and apotosis under normal and low serum culture were detected by flow cytometer.


RESULTS
MSCs were positive for CD44, CD90 and CD71 and negative for CD11b. The cells at G0/G1 cell stage decreased, while the cells at S and G2 cell stage increased in 1 day of 50 mL/L fetal bovine serum (FBS) and 1 to 3 days of 5 mL/L FBS. However, prolonged culture in serum-starvation medium induced cell cycle arrest at G0/G1 stage. The ratio of apoptotic cells did not increase in 50 mL/L FBS. In 5 mL/L FBS, the ratio of apoptotic cells increased in 3 days and then decreased in 4 to 5 days. The proportion of cells at G0/G1 phase was significantly increased from 75.9% to 89.4% while the ratio of apoptotic cells was only 0.162% in 5 days of 5 mL/L FBS.


CONCLUSIONS
It is a good method to induce the synchrony of cell cycle of MSCs at G0/G1 stage in five days of 5 mL/L FBS.
Zhou
Zhu-Juan
ZJ
Department of Neurology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
Zheng
Jian
J
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D005434:Flow Cytometry; D016193:G1 Phase / Q000187:drug effects; D018960:Hyaluronan Receptors / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D011990:Receptors, Transferrin / Q000378:metabolism; D016192:Resting Phase, Cell Cycle / Q000187:drug effects; D018800:Thy-1 Antigens / Q000378:metabolism
D015703:Antigens, CD; C068126:CD71 antigen; D016895:Culture Media, Serum-Free; D018960:Hyaluronan Receptors; D011990:Receptors, Transferrin; D018800:Thy-1 Antigens