Establishment of the enzyme-linked immunosorbent assay system to detect the amino terminal secretory form of rat Erc/Mesothelin.
By representational difference analysis, we previously identified the rat Erc (Expressed in renal carcinoma) gene that was more abundantly expressed in the renal carcinoma tissues of Eker rats than in the rat normal kidney. In this study, we raised antibodies against the amino-terminal portion of the rat Erc, and demonstrated the existence of a approximately 30-kDa secretory form in the supernatant of cultured cells derived from rat renal carcinoma. The enzyme-linked immunosorbent assay (ELISA) system using these antibodies detected high concentrations of this form in the sera of Eker rats bearing renal carcinomas, and in the sera of rats transplanted with mesothelioma cells. Mesothelin, a human homolog of the rat Erc, was recently reported to be a serum marker of malignant mesothelioma. The prognosis of mesothelioma is poor and there is no effective treatment at present. There are several rat model systems of mesothelioma that may be promising tools in the development of an antimesothelioma treatment. We hope our ELISA to detect the soluble form of rat Erc/Mesothelin is useful in the rat model system to exploit the antimesothelioma therapy to be used in human cases.
Nakaishi
Masayuki
M
Department of Urology, Ehime University School of Medicine, Shizukawa, Toh-on, Ehime, Japan.
Kajino
Kazunori
K
Ikesue
Masahiro
M
Hagiwara
Yoshiaki
Y
Kuwahara
Maki
M
Mitani
Hiroaki
H
Horikoshi-Sakuraba
Yuko
Y
Segawa
Tatsuya
T
Kon
Shigeyuki
S
Maeda
Masahiro
M
Wang
Tegexibaiyin
T
Abe
Masaaki
M
Yokoyama
Masayoshi
M
Hino
Okio
O
D048868:Adaptor Proteins, Signal Transducing / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D016466:CHO Cells; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D006224:Cricetinae; D003412:Cricetulus; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D007150:Immunohistochemistry; D007680:Kidney Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D000090204:Mesothelin; D008654:Mesothelioma / Q000097:blood / Q000473:pathology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009374:Neoplasms, Experimental / Q000097:blood / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D048868:Adaptor Proteins, Signal Transducing; C479909:ERC1 protein, rat; C000715529:Msln protein, rat; D011994:Recombinant Proteins; D000090204:Mesothelin