Nonclassical export pathway: overexpression of NCE102 reduces protein and DNA damage and prolongs lifespan in an SGS1 deficient Saccharomyces cerevisiae.
In this study, we used our recently developed screening method, Bud-Scar-based Screening (BSS), to screen a yeast cDNA expression library in an SGS1 deletion BY4742 yeast strain. One gene involved in a nonclassical export pathway, NCE102, was found to extend the life span of Deltasgs1 yeast. Deletion of NCE102 in a wild type yeast strain increased its sensitivity to oxidative stress upon diethylmaleate (DEM) treatment but did not shorten its lifespan, indicating that this gene is not essential in determining yeast lifespan. Transformation of NCE102 into either Deltance102 or Deltasgs1 strains could rescue its tolerance to DEM stress, indicating that NCE102 is protective during oxidative stress. Moreover, overexpression of NCE102 in Deltasgs1 strain leads to reduced protein damage. However, overexpression of NCE102 in wild type yeast strain BY4742 neither protected against oxidative stress due to DEM nor extended yeast lifespan compared to its parental wild type strain, indicating that nonclassical export is redundant and DNA repair is fully sufficient in the wild type strain. We therefore demonstrate that a nonclassical export pathway functions as an alternative clearance/detoxification pathway to eliminate damaged material, when the basic repair pathway is not sufficient.
Desmyter
Liesbeth
L
Department for Molecular Biomedical Research, VIB, Ghent, Belgium.
Verstraelen
Jan
J
Dewaele
Sylviane
S
Libert
Claude
C
Contreras
Roland
R
Chen
Cuiying
C
D000818:Animals; D016922:Cellular Senescence / Q000235:genetics / Q000502:physiology*; D004249:DNA Damage / Q000235:genetics / Q000502:physiology*; D004271:DNA, Fungal / Q000378:metabolism; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal / Q000235:genetics / Q000502:physiology*; D008018:Life Cycle Stages / Q000235:genetics / Q000502:physiology; D008298:Maleates / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects; D053484:RecQ Helicases / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000254:growth & development* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D004718:Saccharomycetales / Q000235:genetics / Q000502:physiology
D004271:DNA, Fungal; D008298:Maleates; C525535:NCE102 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C090472:SGS1 protein, S cerevisiae; D053484:RecQ Helicases; C014476:diethyl maleate