Osteochondral repair using the combination of fibroblast growth factor and amorphous calcium phosphate/poly(L-lactic acid) hybrid materials.
A novel amorphous calcium phosphate (ACP)/poly(L-lactic acid) (PLLA) material, which can experience morphological variations in the microstructure is supposed to be a suitable candidate as scaffold for cartilage tissue-engineering. The purpose of this study was to evaluate the efficacy of this scaffold combined with basic fibroblast growth factor (bFGF) to repair articular cartilage defects in a rabbit model. Forty-two osteochondral defects created in the femoral condyles were (a) left untreated, (b) treated by PLLA combined with bFGF, or (c) ACP/PLLA loaded with bFGF. The treatment of PLLA incorporated with bFGF improved defect filling compared with that left untreated, while the regenerated tissue was mainly fibrocartilage and showed little bone formation with only a small amount of collagen type II (Col II) and no aggrecan gene message measured. When implanted with ACP/PLLA and bFGF, most of the defects were filled with a well-established layer of cartilage tissue with abundance of cartilaginous extracellular matrix accumulation observed. Positive immunohistochemical staining of Col II was observed. High levels of Col II and aggrecan message were also detected by RT-PCR. These results indicate the feasibility of using the combination of ACP/PLLA with bFGF for cartilage repair.
Huang
Xin
X
Department of Orthopaedic Surgery, The Second Affiliated Hospital, Medical College of Zhejiang University, Hangzhou 310009, China.
Yang
Disheng
D
Yan
Weiqi
W
Shi
Zhongli
Z
Feng
Jie
J
Gao
Yanbo
Y
Weng
Wenjian
W
Yan
Shigui
S
D053674:Aggrecans / Q000235:genetics; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D002130:Calcium Phosphates / Q000737:chemistry*; D002358:Cartilage, Articular / Q000378:metabolism / Q000601:surgery; D024042:Collagen Type I / Q000032:analysis / Q000235:genetics; D024043:Collagen Type II / Q000032:analysis / Q000235:genetics; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D019344:Lactic Acid / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D011817:Rabbits; D011897:Random Allocation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D023822:Tissue Engineering / Q000379:methods; D014945:Wound Healing / Q000187:drug effects
D053674:Aggrecans; D001672:Biocompatible Materials; D002130:Calcium Phosphates; D024042:Collagen Type I; D024043:Collagen Type II; D011108:Polymers; C519480:amorphous calcium phosphate; D016222:Fibroblast Growth Factor 2; D019344:Lactic Acid; C020243:calcium phosphate; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)