title: [Comparison of multilocus sequence typing system and pulsed-field gel electrophoresis in typing of Salmonella enteritidis].
abstract: OBJECTIVE
  To compare the discriminatory ability between multilocus sequence typing system (MLST) and pulsed-field gel electrophoresis (PFGE).
  
  
  METHODS
  Salmonella enteritidis strains, isolated from food in China were identified by MLST, under PCR products of thrA, purE, sucA, aroC, hemD, dnaN and Sdf I. The same set of strains was typed by PFGE using Xba I, Spe I as the restriction enzyme in order to compare the discriminatory power of the methods.
  
  
  RESULTS
  Data from MLST revealed the lack of diversity among the strains of the same serotype and the number of variable nucleotide sites per locus ranged from 1 to 23 between Salmonella typhi LT2 and other serotypes of Salmonella. However, 50 Salmonella enteritidis strains were identified as 11 patterns and more sub-patterns by PFGE.
  
  
  CONCLUSIONS
  In strain typing, PFGE was the highly discriminatory method comparing to the MLST system.
authors.affiliation: National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China. jirong36@126.com
authors.forename: Rong
authors.initials: R
authors.lastname: Ji
authors.forename: Yan-jun
authors.initials: YJ
authors.lastname: Li
authors.forename: Yu-ping
authors.initials: YP
authors.lastname: Wang
authors.forename: Sheng-hui
authors.initials: SH
authors.lastname: Cui
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
mesh_terms: D016521:Electrophoresis, Gel, Pulsed-Field; D005798:Genes, Bacterial; D016133:Polymerase Chain Reaction; D012477:Salmonella enteritidis / Q000145:classification* / Q000235:genetics; D017422:Sequence Analysis, DNA; D012703:Serotyping