title: E-cadherin expression and bromodeoxyuridine incorporation during development of ovarian inclusion cysts in age-matched breeder and incessantly ovulated CD-1 mice.
abstract: BACKGROUND
  Female CD-1/Swiss Webster mice subjected to incessant ovulation for 8 months and 12-month breeder mice both developed ovarian inclusion cysts similar to serous cystadenomas. The majority of cysts appeared to be dilated rete ovarii tubules, but high ovulation number resulted in more cortical inclusion cysts. We hypothesized that comparison of inclusion cyst pathology in animals of the same age, but with differences in total lifetime ovulation number, might allow us to determine distinguishing characteristics of the two types of cyst.
  
  
  METHODS
  Ovaries from breeder mice (BR) or females subjected to incessant ovulation (IO) were compared at 6-, 9- and 12-months of age. Ovaries were serially sectioned and cysts characterized with regard to location and histology, E-cadherin immunoreactivity and rates of BrdU incorporation.
  
  
  RESULTS
  Inclusion cysts developed with age in BR and IO ovaries. The majority of cysts were connected to the ovarian hilus. Two cortical inclusion cysts were observed in ten IO ovaries and one in ten BR ovaries. Low or no E-cadherin immuno-staining was seen in the OSE of all mice studied. Conversely, strong membrane immuno-staining was observed in rete ovarii epithelial cells. Variable E-cadherin immunoreactivity was seen in cells of hilar inclusion cysts, with strong staining observed in cuboidal ciliated cells and little or no staining in flat epithelial cells. Two of the three cortical cysts contained papillae, which showed E-cadherin immuno-staining at the edge of cells. However hilar and cortical cysts were not distinguishable by morphology, cell type or E-cadherin immunoreactivity. BrdU incorporation in cyst cells (1.4% [95% CI: 1.0 to 2.1]) was greater than in OSE (0.7% [95% CI: 0.4 to 1.2]) and very few BrdU-labeled cells were observed in rete ovarii at any age. Incessant ovulation significantly increased BrdU incorporation in OSE of older animals.
  
  
  CONCLUSIONS
  These experiments confirm ovarian inclusion cysts develop with age in the CD-1 mouse strain, irrespective of total ovulation burden. We conclude longer periods of incessant ovulation do not lead to significant changes in inclusion cyst formation or steroidogenesis in CD-1 mice and inclusion cyst type can not be distinguished by morphology, cell proliferation rate or E-cadherin immunoreactivity.
authors.affiliation: Eskitis Institute of Cell & Molecular Therapies, Griffith University Nathan campus, Nathan, Australia. j.fleming@griffith.edu.au
authors.forename: Jean S
authors.initials: JS
authors.lastname: Fleming
authors.forename: H James
authors.initials: HJ
authors.lastname: McQuillan
authors.forename: Melanie J
authors.initials: MJ
authors.lastname: Millier
authors.forename: Clare R
authors.initials: CR
authors.lastname: Beaugié
authors.forename: Vicki
authors.initials: V
authors.lastname: Livingstone
mesh_terms: D000375:Aging / Q000097:blood / Q000378:metabolism; D000735:Androstenedione / Q000097:blood; D000818:Animals; D017209:Apoptosis; D001947:Breeding; D001973:Bromodeoxyuridine / Q000378:metabolism*; D015820:Cadherins / Q000378:metabolism*; D020420:Cyst Fluid / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D004958:Estradiol / Q000097:blood / Q000378:metabolism; D005260:Female; D015151:Immunoblotting; D007120:Immunochemistry; D051379:Mice; D008815:Mice, Inbred Strains; D009994:Osmolar Concentration; D010048:Ovarian Cysts / Q000209:etiology* / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D010053:Ovary / Q000378:metabolism; D010060:Ovulation; D013045:Species Specificity; D013739:Testosterone / Q000097:blood
chemical_list: D015820:Cadherins; D013739:Testosterone; D000735:Androstenedione; D004958:Estradiol; D001973:Bromodeoxyuridine