Interaction of the selectin ligand PSGL-1 with chemokines CCL21 and CCL19 facilitates efficient homing of T cells to secondary lymphoid organs.
P-selectin glycoprotein ligand 1 (PSGL-1) is central to the trafficking of immune effector cells to areas of inflammation through direct interactions with P-selectin, E-selectin and L-selectin. Here we show that PSGL-1 was also required for efficient homing of resting T cells to secondary lymphoid organs but functioned independently of selectin binding. PSGL-1 mediated an enhanced chemotactic T cell response to the secondary lymphoid organ chemokines CCL21 and CCL19 but not to CXCL12 or to inflammatory chemokines. Our data show involvement of PSGL-1 in facilitating the entry of T cells into secondary lymphoid organs, thereby demonstrating the bifunctional nature of this molecule.
Veerman
Krystle M
KM
The Biomedical Research Centre, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada.
Williams
Michael J
MJ
Uchimura
Kenji
K
Singer
Mark S
MS
Merzaban
Jasmeen S
JS
Naus
Silvia
S
Carlow
Douglas A
DA
Owen
Philip
P
Rivera-Nieves
Jesús
J
Rosen
Steven D
SD
Ziltener
Hermann J
HJ
D000818:Animals; D002448:Cell Adhesion / Q000235:genetics / Q000276:immunology; D054415:Chemokine CCL19; D054421:Chemokine CCL21; D019742:Chemokines, CC / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D006706:Homeostasis / Q000502:physiology*; D008221:Lymphoid Tissue / Q000166:cytology* / Q000276:immunology; D008562:Membrane Glycoproteins / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D013601:T-Lymphocytes / Q000502:physiology*
C515461:Ccl19 protein, mouse; C515479:Ccl21c protein, mouse; D054415:Chemokine CCL19; D054421:Chemokine CCL21; D019742:Chemokines, CC; D008562:Membrane Glycoproteins; C084882:P-selectin ligand protein