Isolation of fatty acids with anticancer activity from Protaetia brevitarsis larva.
In this study, biologically active compounds were isolated from Protaetia brevitarsis larva (PBL) by dichloromethane extraction. The dichloromethane extract from PBL was highly cytotoxic to various cancer cells. From a silica gel column chromatograpy of this extract, we obtained four fractions (F-2, F-4, F-5 and F-7) having apoptosis-inducing activity. These fractions induced DNA ladder and caspase-3 activation during apoptosis in colon 26 tumor cells. In 1H and 13C NMR and mass spectral analysis of the fraction F-2 showing the highest apoptosis-inducing activity, we found that the fraction was composed of three free fatty acids such as palmitic acid, (Z)-9-octadecenoic acid and octadecenoic acid. These results indicate that the dichloromethane extract of PBL includes anticancer components composed of at least three fatty acids, and apoptosis-inducing activity of the extract was mediated by caspase-3 activation in tumor cells.
Yoo
Yung-Choon
YC
Department of Biochemistry, College of Medicine, Konyang University, Daejeon 302-801, Korea.
Shin
Byung-Hoon
BH
Hong
Jang-Hee
JH
Lee
Junglim
J
Chee
Hee-Youn
HY
Song
Kyung-Sik
KS
Lee
Kyung-Bok
KB
D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D001517:Coleoptera / Q000737:chemistry*; D005227:Fatty Acids / Q000302:isolation & purification* / Q000494:pharmacology; D007814:Larva; D051379:Mice
D000970:Antineoplastic Agents; D005227:Fatty Acids; D053148:Caspase 3