Induction of apoptosis in human breast adenocarcinoma MCF-7 cells by pterocarnin A from the bark of Pterocarya stenoptera via the Fas-mediated pathway.
Pterocarnin A, isolated from the bark of Pterocarya stenoptera (Juylandaceae), was investigated for its antiproliferative activity in human breast adenocarcinoma MCF-7 cells. To identify the anticancer mechanism of pterocarnin A, we assayed its effects on apoptosis, cell cycle distribution, and levels of p53, p21/WAF1, Fas/APO-1 receptor and Fas ligand. The results showed that pterocarnin A induced apoptosis of MCF-7 cells without mediation of p53 and p21/WAF1. We suggest that the Fas/Fas ligand apoptotic system is the main pathway of pterocarnin A-mediated apoptosis of MCF-7 cells. Our study reports here for the first time that the activity of the Fas/Fas ligand apoptotic system may participate in the antiproliferative activity of pterocarnin A in MCF-7 cells.
Kuo
Po-Lin
PL
Department of Biotechnology, Cell Biology Laboratory, Institute of Cosmetic Science, Chia-Nan University of Pharmacy and Science, Tainan, Taiwan, ROC.
Hsu
Ya-Ling
YL
Sung
Shu-Chiao
SC
Ni
Wen-Chiu
WC
Lin
Ta-Chen
TC
Lin
Chun-Ching
CC
D000230:Adenocarcinoma / Q000188:drug therapy* / Q000473:pathology; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D053181:Caspase 8 / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D053222:Fas Ligand Protein / Q000502:physiology*; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D016158:Genes, p53 / Q000235:genetics; D006027:Glycosides / Q000494:pharmacology*; D006801:Humans; D029525:Juglandaceae / Q000737:chemistry*; D015689:Oncogene Protein p21(ras) / Q000235:genetics; D024301:Plant Bark / Q000737:chemistry
D000972:Antineoplastic Agents, Phytogenic; D053222:Fas Ligand Protein; D006027:Glycosides; C496413:pterocarnin A; D005707:Gallic Acid; D053181:Caspase 8; D015689:Oncogene Protein p21(ras)