Development of high-throughput phenotyping of metagenomic clones from the human gut microbiome for modulation of eukaryotic cell growth.
Metagenomic libraries derived from human intestinal microbiota (20,725 clones) were screened for epithelial cell growth modulation. Modulatory clones belonging to the four phyla represented among the metagenomic libraries were identified (hit rate, 0.04 to 8.7% depending on the screening cutoff). Several candidate loci were identified by transposon mutagenesis and subcloning.
Gloux
Karine
K
Unité d'Ecologie et de Physiologie du Système Digestif, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France.
Leclerc
Marion
M
Iliozer
Harout
H
L'Haridon
René
R
Manichanh
Chaysavanh
C
Corthier
Gérard
G
Nalin
Renaud
R
Blottière
Hervé M
HM
Doré
Joël
J
D000818:Animals; D001419:Bacteria / Q000235:genetics*; D002460:Cell Line; D049109:Cell Proliferation; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004847:Epithelial Cells / Q000382:microbiology; D005057:Eukaryotic Cells / Q000382:microbiology*; D015870:Gene Expression; D016680:Genome, Bacterial; D023281:Genomics / Q000379:methods*; D006801:Humans; D007422:Intestines / Q000382:microbiology*
D004269:DNA, Bacterial