Promise of multiphoton detection in discovery and diagnostic proteomics.
Proteomics has lacked adequate methods for handling the complexity (hundreds of thousands of different proteins) and range of protein concentrations (> or =10(6)) of eukaryotic proteomes. New multiphoton-detection methods for ultrasensitive detection of proteins produce 10,000-fold gains in sensitivity and allow highly quantitative, linear detection of 50 zmol (30,000 molecules) to 500 fmol of proteins in complex samples. The potential of multiphoton detection in top-down proteomics analyses is illustrated with applications in monitoring proteomes in very small numbers of cells, in identifying and monitoring complex functional isoforms of cancer-related proteins, and in super-sensitive immunoassays of serum proteins for high-performance detection of cancer.
Godovac-Zimmermann
Jasminka
J
University College London, Department of Medicine, Centre for Molecular Medicine, London, UK. j.godovac-zimmermann@ucl.ac.uk
Mulvey
Claire
C
Konstantoulaki
Maria
M
Sainsbury
Richard
R
Brown
Larry R
LR
D001798:Blood Proteins / Q000032:analysis; D019937:Diagnostic Techniques and Procedures; D006801:Humans; D007457:Iodine Radioisotopes / Q000032:analysis*; D008722:Methods; D009363:Neoplasm Proteins / Q000032:analysis; D017785:Photons; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000295:instrumentation / Q000379:methods* / Q000592:standards / Q000639:trends
D001798:Blood Proteins; D007457:Iodine Radioisotopes; D009363:Neoplasm Proteins; D011506:Proteins