title: Genotyping of Giardia duodenalis isolates among residents of slum area in Manila, Philippines.
abstract: Giardia duodenalis is a flagellated protist that causes gastrointestinal disease throughout the world. In the Philippines, study on G. duodenalis is limited. It is also believed that prevalence rates of this organism in the country are underestimated. In this study, stool samples from residents living in a slum area in Manila were collected. These were examined under microscopy for identification of common helminthic and protistan parasites. Results showed that 22.05% of 2,354 stool samples collected contained Giardia cysts. A fraction of samples (n = 133) positive for Giardia cysts were set aside. Genomic DNA was extracted from these samples and a polymerase chain reaction-restriction fragment length polymorphism procedure based on the organism's triose phosphate isomerase gene was utilized. This particular procedure is capable of distinguishing assemblages or genotypes within G. duodenalis. The highest identified assemblage was Assemblage B (86.47%). The two genotypes of Assemblage A were also detected. This is the first report on the identification of genotypes of G. duodenalis in the Philippines. The results of this study can serve as basis for future control and prevention of giardiasis and parasitism in the country.
authors.affiliation: Institute of Biology, College of Science, University of the Philippines, Diliman, Quezon City 1101, Philippines.
authors.forename: John Anthony D L
authors.initials: JA
authors.lastname: Yason
authors.forename: Windell L
authors.initials: WL
authors.lastname: Rivera
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D002648:Child; D002675:Child, Preschool; D016054:DNA, Protozoan / Q000032:analysis / Q000302:isolation & purification; D005243:Feces; D005260:Female; D005838:Genotype; D005872:Giardia / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005873:Giardiasis / Q000453:epidemiology* / Q000469:parasitology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008969:Molecular Sequence Data; D010679:Philippines; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011204:Poverty Areas; D015800:Protozoan Proteins / Q000235:genetics; D014305:Triose-Phosphate Isomerase / Q000235:genetics*
chemical_list: D016054:DNA, Protozoan; D015800:Protozoan Proteins; D014305:Triose-Phosphate Isomerase