[Transduction peptides: structural-functional analyses in model membranes].
Peptide-membrane interaction is the first step required for peptide cell internalization. In this paper we studied the interactions of substance P, Penetratin and an amphiphilic 16mer (RL16) peptide in two different model membranes, giant unilamellar vesicles and large unilamellar vesicles. Penetratin was able to induce the formation of tubes inside the giant vesicles without changes in membrane permeability. On the contrary, RL16 induced the disruption of giant vesicles and the permeabilization of large vesicles. Substance P showed none of these effects.
Lamazière
Antonin
A
UMR Inserm U538, CHU Saint Antoine, Université Pierre et Marie Curie, 27 rue de Chaligny, 75012 Paris, France.
Chassaing
Gérard
G
Trugnan
Germain
G
Ayala-Sanmartin
Jesus
J
D001704:Biopolymers; D002352:Carrier Proteins / Q000502:physiology; D057846:Cell-Penetrating Peptides; D004339:Drug Compounding; D005452:Fluoresceins / Q000378:metabolism; D008081:Liposomes; D008563:Membrane Lipids; D008567:Membranes, Artificial; D010455:Peptides / Q000737:chemistry / Q000502:physiology*; D010539:Permeability; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D010940:Plant Proteins / Q000737:chemistry / Q000502:physiology*; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D013373:Substance P / Q000502:physiology
D001704:Biopolymers; D002352:Carrier Proteins; D057846:Cell-Penetrating Peptides; D005452:Fluoresceins; D008081:Liposomes; D008563:Membrane Lipids; D008567:Membranes, Artificial; D010455:Peptides; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D010940:Plant Proteins; C015462:polyarginine; C027819:polyleucine; D013373:Substance P; C414409:penetratin; C007740:fluorexon