Northern analysis of sense and antisense frequency RNA in Neurospora crassa.
In Northern analysis the presence of specific RNA transcripts is detected and their quantity can be estimated. RNA is separated using denaturing agarose gel electrophoresis and is subsequently transferred and fixed to a solid support, such as a nitrocellulose filter. When labeled probes are hybridized to these immobilized RNA molecules, their presence can be visualized by autoradiography. Here we describe Northern hybridization using radioactively labeled riboprobes to show circadian expression of endogenous sense and antisense frequency RNA in the filamentous fungus Neurospora crassa.
Kramer
Cas
C
Department of Genetics, University of Leicester, UK.
Crosthwaite
Susan K
SK
D015152:Blotting, Northern / Q000379:methods*; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D009492:Neurospora crassa / Q000235:genetics*; D015347:RNA Probes; D016372:RNA, Antisense / Q000032:analysis / Q000235:genetics; D012331:RNA, Fungal / Q000032:analysis* / Q000235:genetics*
C087405:FRQ protein, Neurospora crassa; D005656:Fungal Proteins; D015347:RNA Probes; D016372:RNA, Antisense; D012331:RNA, Fungal