Signalling pathways underlying neural cell adhesion molecule-mediated survival of dopaminergic neurons.
Stimulation of the neural cell adhesion molecule (NCAM) by homophilic interactions is known to lead to neurite outgrowth as well as to neuronal survival. Whereas a complex network of signalling molecules is known to be of importance to NCAM-mediated neurite extension, only limited information is available regarding signalling underlying NCAM-mediated neuroprotection. Here, we present data suggesting a difference in the signalling events required for survival of rat dopaminergic neurons as compared with neurite outgrowth from the same cell type. Whereas Fyn, fibroblast growth factor receptor, mitogen-activated protein and ERK kinase, protein kinase A and protein kinase C are required for both responses to NCAM-induced signalling, phospholipase C and Ca(2+)-calmodulin-dependent kinase II are only necessary for the neurite outgrowth response, but dispensable for neuroprotection.
Ditlevsen
Dorte Kornerup
DK
Protein Laboratory, Department of Neuroscience and Pharmacology, University of Copenhagen, Panum Institute 6.2, Blegdamsvej 3, Copenhagen, Denmark. dorted@plab.ku.dk
Berezin
Vladimir
V
Bock
Elisabeth
E
D018663:Adrenergic Agents / Q000633:toxicity; D000704:Analysis of Variance; D000818:Animals; D002452:Cell Count / Q000379:methods; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D015933:Complement C3d / Q000494:pharmacology; D004298:Dopamine / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004622:Embryo, Mammalian; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008636:Mesencephalon / Q000166:cytology; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D018696:Neuroprotective Agents / Q000494:pharmacology; D016627:Oxidopamine / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000502:physiology*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D018663:Adrenergic Agents; D004791:Enzyme Inhibitors; D019006:Neural Cell Adhesion Molecules; D018696:Neuroprotective Agents; D015933:Complement C3d; D016627:Oxidopamine; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine