Differential responses of Coffea arabica L. leaves and roots to chemically induced systemic acquired resistance.
Coffea arabica is susceptible to several pests and diseases, some of which affect the leaves and roots. Systemic acquired resistance (SAR) is the main defence mechanism activated in plants in response to pathogen attack. Here, we report the effects of benzo(1,2,3)thiadiazole-7-carbothioic acid-S-methyl ester (BTH), a SAR chemical inducer, on the expression profile of C. arabica. Two cDNA libraries were constructed from the mRNA isolated from leaves and embryonic roots to create 1587 nonredundant expressed sequence tags (ESTs). We developed a cDNA microarray containing 1506 ESTs from the leaves and embryonic roots, and 48 NBS-LRR (nucleotide-binding site leucine-rich repeat) gene fragments derived from 2 specific genomic libraries. Competitive hybridization between untreated and BTH-treated leaves resulted in 55 genes that were significantly overexpressed and 16 genes that were significantly underexpressed. In the roots, 37 and 42 genes were over and underexpressed, respectively. A general shift in metabolism from housekeeping to defence occurred in the leaves and roots after BTH treatment. We observed a systemic increase in pathogenesis-related protein synthesis, in the oxidative burst, and in the cell wall strengthening processes. Moreover, responses in the roots and leaves varied significantly.
De Nardi
Barbara
B
Department of Biology, University of Trieste, Trieste, Italy.
Dreos
René
R
Del Terra
Lorenzo
L
Martellossi
Chiara
C
Asquini
Elisa
E
Tornincasa
Patrizia
P
Gasperini
Debora
D
Pacchioni
Beniamina
B
Rathinavelu
Rajkumar
R
Pallavicini
Alberto
A
Graziosi
Giorgio
G
D040503:Coffea / Q000187:drug effects* / Q000235:genetics* / Q000276:immunology; D020224:Expressed Sequence Tags; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000502:physiology; D007113:Immunity, Innate / Q000187:drug effects* / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D018515:Plant Leaves / Q000187:drug effects* / Q000235:genetics; D018517:Plant Roots / Q000187:drug effects* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013830:Thiadiazoles / Q000494:pharmacology*
D013830:Thiadiazoles; C099403:S-methyl benzo(1,2,3)thiadiazole-7-carbothioate