PCR examination of bronchoalveolar lavage samples is a useful tool in pre-clinical diagnosis of ovine pulmonary adenocarcinoma (Jaagsiekte).
Ovine pulmonary adenocarcinoma (OPA) is a contagious lung tumour of sheep caused by Jaagsiekte sheep retrovirus (JSRV). The disease is a particular problem in flocks in many parts of the world. The aim of the study was to assess screening methods for individual animals as a prelude to future eradication trials. Results of histological examination were used as the standard to evaluate the relative sensitivity and specificity of an established heminested polymerase chain reaction (PCR) test for JSRV proviral DNA from blood and bronchoalveolar lavage (BAL) samples. PCR results from tissue samples are included as control data. PCR testing of blood samples was found to have an estimated sensitivity of only 10% (95% confidence interval (CI) 3-20) while the sensitivity of the PCR test on BAL samples was 89% (CI 79-96) in comparison to the results of histological examination. We conclude that PCR testing of BAL samples is an effective confirmatory test for sheep with suspected clinical OPA. It is also a useful tool for the pre-clinical identification of individual infected sheep within an infected flock and therefore may prove beneficial in future control or eradication programmes.
Voigt
K
K
Clinic for Swine, Small Ruminants, Forensic Medicine and Ambulatory Services, University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, 30173 Hannover, Germany. katja.voigt@sac.co.uk
Brügmann
M
M
Huber
K
K
Dewar
P
P
Cousens
C
C
Hall
M
M
Sharp
J M
JM
Ganter
M
M
D000818:Animals; D018893:Bronchoalveolar Lavage / Q000191:economics / Q000662:veterinary; D001992:Bronchoalveolar Lavage Fluid / Q000821:virology*; D005260:Female; D022141:Jaagsiekte sheep retrovirus / Q000302:isolation & purification*; D016676:Macrophages, Alveolar / Q000821:virology; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D011237:Predictive Value of Tests; D011648:Pulmonary Adenomatosis, Ovine / Q000175:diagnosis* / Q000821:virology; D012680:Sensitivity and Specificity; D012756:Sheep