title: [Amplification of proviral DNA of bovine immunodeficiency virus in experimentally infected animals].
abstract: The primer set for detection of bovine immunodeficiency virus (BIV) proviral DNA, retrovirus of unknown pathology, by standard polymerase chain reaction was developed. Amplicon of the expected size was detected in DNA from peripheral blood mononuclear cells of seropositive experimentally BIV infected sheep and cattle. Primers targeted the short fragment of BIV pol gene. Sequences of BIV proviral DNA extracted from BIV infected cell culture as well as from experimentally infected cows were taken for targeted pol BI BPX gene locus. Problems of BIV detection from the clinical material of the experimentally and naturally infected animals are discussed.
authors.forename: O Iu
authors.initials: OIu
authors.lastname: Lymans'ka
authors.forename: M
authors.initials: M
authors.lastname: Rola
authors.forename: L
authors.initials: L
authors.lastname: Byka
authors.forename: Ia
authors.initials: Ia
authors.lastname: Kuzmak
authors.forename: Iu L
authors.initials: IuL
authors.lastname: Volians'kyĭ
authors.forename: N B
authors.initials: NB
authors.lastname: Puhach
authors.forename: S V
authors.initials: SV
authors.lastname: Novikov
authors.forename: O P
authors.initials: OP
authors.lastname: Lymans'kyĭ
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology; D017931:DNA Primers / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D004195:Disease Models, Animal; D016087:Immunodeficiency Virus, Bovine / Q000235:genetics / Q000302:isolation & purification*; D016180:Lentivirus Infections / Q000662:veterinary / Q000821:virology*; D008214:Lymphocytes / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D011533:Proviruses / Q000235:genetics / Q000302:isolation & purification*; D012756:Sheep; D012757:Sheep Diseases / Q000821:virology
chemical_list: D017931:DNA Primers; D004279:DNA, Viral