title: Comparative analysis of serum proteomes to discover biomarkers for ossification of the posterior longitudinal ligament.
abstract: METHODS
  Serum proteomes from normal subjects and the patients with ossification of the posterior longitudinal ligament (OPLL) were analyzed by using proteomics.
  
  
  OBJECTIVE
  To identify novel serologic biomarkers for diagnosing OPLL.
  
  
  BACKGROUND
  OPLL can compress the spinal cord, and special planning is required for surgeries that are done from the front of the cervical spine. However, the definitive serologic biomarkers for OPLL are still unclear.
  
  
  METHODS
  The 2-dimensional electrophoresis patterns of sera from OPLL patients and normal subjects were compared. The differentially expressed spots were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and electrospray ionization quadruple time-of-flight mass spectrometry.
  
  
  RESULTS
  Nine spots that were differentially expressed in the sera of OPLL patients were found and were identified. PRO2675, human serum albumin in a complex with myristic acid and tri-iodobenzoic acid, an unknown protein, chain B of the crystal structure of deoxy-human hemoglobin beta6, pro-apolipoprotein, ALB protein, retinol binding protein and chain A of human serum albumin mutant R218h complexed with thyroxine (3,3',5,5', tetraiodo-L-thyronine), were up-regulated in the sera of OPLL patients, whereas alpha1-microglobulin/bikunin precursor was down-regulated.
  
  
  CONCLUSIONS
  These proteins could be used as diagnostic biomarkers of OPLL.
authors.affiliation: Department of Pharmacology, Research Institute of Clinical Medicine, Chonbuk National University Medical School, Jeonbuk, Republic of Korea.
authors.forename: Jong-Pil
authors.initials: JP
authors.lastname: Eun
authors.forename: Tian-Ze
authors.initials: TZ
authors.lastname: Ma
authors.forename: Woo-Jong
authors.initials: WJ
authors.lastname: Lee
authors.forename: Min-Gul
authors.initials: MG
authors.lastname: Kim
authors.forename: Min-Jeong
authors.initials: MJ
authors.lastname: Yoo
authors.forename: Eun-Jung
authors.initials: EJ
authors.lastname: Koh
authors.forename: Ha-Young
authors.initials: HY
authors.lastname: Choi
authors.forename: Yong-Geun
authors.initials: YG
authors.lastname: Kwak
mesh_terms: D000328:Adult; D001053:Apolipoproteins / Q000097:blood / Q000235:genetics; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006023:Glycoproteins / Q000097:blood / Q000235:genetics; D006454:Hemoglobins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D008875:Middle Aged; D017887:Ossification of Posterior Longitudinal Ligament / Q000097:blood* / Q000175:diagnosis*; D011498:Protein Precursors / Q000097:blood / Q000235:genetics; D020543:Proteome / Q000378:metabolism*; D012177:Retinol-Binding Proteins / Q000235:genetics / Q000378:metabolism; D012709:Serum Albumin / Q000235:genetics / Q000378:metabolism; D000075462:Serum Albumin, Human; D015854:Up-Regulation
chemical_list: C422455:ALB protein, human; D001053:Apolipoproteins; D015415:Biomarkers; D006023:Glycoproteins; D006454:Hemoglobins; D011498:Protein Precursors; D020543:Proteome; D012177:Retinol-Binding Proteins; D012709:Serum Albumin; C100003:deoxyhemoglobin; D000075462:Serum Albumin, Human