A choice behavior for morphine reveals experience-dependent drug preference and underlying neural substrates in developing larval zebrafish.
Transparent larval zebrafish offer the opportunity to unravel genetic and neuronal networks underlying behavior in a developing system. In this study, we developed a choice chamber paradigm to measure reward-associated behavior in larval zebrafish. In the chamber where larval zebrafish have a choice of spending their time in either a water- or morphine-containing compartment, larvae that have previously experienced morphine spend significantly more time in the compartment containing morphine. This behavior can be attentuated by pre-treatment with antagonists of the opioid receptor or the dopamine receptor, and furthermore, is impaired in the too few mutant, which has a genetic deficiency in the production of specific groups of dopaminergic and serotonergic neurons in the ventral forebrain. These results uncover a choice behavior for an addictive substance in larval zebrafish that is mediated through central opioid and monoaminergic neurotransmitter systems.
Bretaud
S
S
Department of Biopharmaceutical Sciences, University of California, San Francisco, CA 94143-2811, USA.
Li
Q
Q
Lockwood
B L
BL
Kobayashi
K
K
Lin
E
E
Guo
S
S
D000701:Analgesics, Opioid / Q000494:pharmacology*; D000818:Animals; D001679:Biogenic Amines / Q000502:physiology; D002755:Choice Behavior / Q000187:drug effects*; D002853:Chromatography, Liquid; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D004298:Dopamine / Q000502:physiology; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007814:Larva / Q000502:physiology*; D013058:Mass Spectrometry; D009020:Morphine / Q000494:pharmacology*; D009043:Motor Activity / Q000502:physiology; D009270:Naloxone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D009415:Nerve Net / Q000502:physiology; D018377:Neurotransmitter Agents / Q000502:physiology; D018035:Receptors, Odorant / Q000235:genetics / Q000502:physiology; D011957:Receptors, Opioid / Q000502:physiology; D017450:Receptors, Opioid, mu / Q000235:genetics / Q000502:physiology; D012054:Reinforcement, Psychology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012201:Reward; D015398:Signal Transduction / Q000502:physiology; D015027:Zebrafish / Q000502:physiology*
D000701:Analgesics, Opioid; D001679:Biogenic Amines; D009292:Narcotic Antagonists; D018377:Neurotransmitter Agents; D018035:Receptors, Odorant; D011957:Receptors, Opioid; D017450:Receptors, Opioid, mu; D009270:Naloxone; D009020:Morphine; D004298:Dopamine