Implementation of a one-step real-time RT-PCR protocol for diagnosis of foot-and-mouth disease.
An automated one-step real-time reverse transcription polymerase chain reaction (rRT-PCR) protocol was optimised and evaluated for the routine diagnosis of foot-and-mouth disease (FMD). Parallel testing of RNA samples (n=257) indicated that this assay has a diagnostic sensitivity at least equivalent to the automated two-step rRT-PCR protocol previously used for the laboratory detection of FMD virus (FMDV). This more rapid and economical one-step protocol will play a key role in contingency planning for any future outbreaks of FMD in the United Kingdom (UK).
Shaw
Andrew E
AE
Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Woking, Surrey, GU24 0NF, United Kingdom.
Reid
Scott M
SM
Ebert
Katja
K
Hutchings
Geoffrey H
GH
Ferris
Nigel P
NP
King
Donald P
DP
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D005536:Foot-and-Mouth Disease / Q000175:diagnosis*; D029544:Foot-and-Mouth Disease Virus / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D006113:United Kingdom