title: Genetic reconstruction of a functional transcriptional regulatory network.
abstract: Although global analyses of transcription factor binding provide one view of potential transcriptional regulatory networks, regulation also occurs at levels distinct from transcription factor binding. Here, we use a genetic approach to identify targets of transcription factors in yeast and reconstruct a functional regulatory network. First, we profiled transcriptional responses in S. cerevisiae strains with individual deletions of 263 transcription factors. Then we used directed-weighted graph modeling and regulatory epistasis analysis to identify indirect regulatory relationships between these transcription factors, and from this we reconstructed a functional transcriptional regulatory network. The enrichment of promoter motifs and Gene Ontology annotations provide insight into the biological functions of the transcription factors.
authors.affiliation: Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, Section of Molecular Genetics and Microbiology, University of Texas at Austin, 1 University Station A4800, Austin, Texas 78712, USA.
authors.forename: Zhanzhi
authors.initials: Z
authors.lastname: Hu
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Killion
authors.forename: Vishwanath R
authors.initials: VR
authors.lastname: Iyer
mesh_terms: D001483:Base Sequence; D004843:Epistasis, Genetic; D015870:Gene Expression; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks / Q000235:genetics*; D008957:Models, Genetic; D020411:Oligonucleotide Array Sequence Analysis; D011401:Promoter Regions, Genetic / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012984:Software; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D014157:Transcription Factors