title: Enhancement effect of polyoxometalates on NGF-induced neurite-outgrowth of PC12 cells.
abstract: Nerve growth factor (NGF)-induced neurite-outgrowth of PC12 cells was enhanced by polyoxometalates such as Na9[SbW(9)O(33)].19.5H(2)O (SbW(9)) and (NH(3)Pr(i))6[Mo(7)O(24)].3H(2)O (Mo(7)). Western blotting analysis of polyoxometalate/NGF-treated PC12 cells showed a strong expression of growth-associated protein 43 (GAP-43), which is associated with the neurite outgrowth. Similar effects were observed for other polyoxometalates, K(11)H[(VO)3(SbW(9)O(33))2].27H(2)O ((VO)3(SbW(9))2), K6[P(2)W(18)O(62)].14H(2)O (P(2)W(18)), and K7[PTi(2)W(10)O(40)].6H2O (PTi(2)W(10)), in contrast with little effect for monomeric tungstate and molybdate. Of the polyoxometalates tested in this study, SbW(9) and Mo(7) were the most potent.
authors.affiliation: Chemical Resources Laboratory, Tokyo Institute of Technology, Japan.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Oda
authors.forename: Miyao
authors.initials: M
authors.lastname: Inoue
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Hino
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Yamase
mesh_terms: D000818:Animals; D000965:Antimony / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D019922:GAP-43 Protein / Q000378:metabolism; D008982:Molybdenum / Q000494:pharmacology*; D020932:Nerve Growth Factor / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects*; D009942:Organometallic Compounds / Q000494:pharmacology*; D016716:PC12 Cells; D051381:Rats; D013997:Time Factors
chemical_list: D015415:Biomarkers; D019922:GAP-43 Protein; D009942:Organometallic Compounds; D008982:Molybdenum; D020932:Nerve Growth Factor; D000965:Antimony