title: Vascular stiffness and genetic variation at the endothelial nitric oxide synthase locus: the Framingham Heart study.
abstract: Arterial stiffness is a moderately heritable trait that is affected by alterations in the bioavailability of NO. Previous studies have found associations between variants in the gene for endothelial NO synthase (NOS3) and arterial properties. We previously identified a linkage peak for forward pressure wave amplitude in the immediate vicinity of NOS3. Therefore, we evaluated relations between arterial stiffness measures and common genetic variants at this locus. Eighteen single nucleotide polymorphisms capturing approximately 90% of underlying common variation in NOS3 were genotyped in unrelated Framingham Heart Study participants (N=1157; 52.2% women; mean age: 62 years) with routinely ascertained tonometry data that provided 5 arterial phenotypes (forward and reflected pressure wave amplitude, central pulse pressure, carotid-femoral pulse wave velocity, and mean arterial pressure). In women but not men, the genotype for the common NOS3 missense mutation (Glu298Asp, rs1799983) was related to central pulse pressure (women: GG=53+/-0.9, GT=54+/-0.9, and TT=47+/-2.0 mm Hg, P=0.0047; men: GG=50+/-1.0, GT=49+/-0.9, and TT=47+/-1.8 mm Hg, P=0.30) and forward wave amplitude (women: GG=41+/-0.7, GT=42+/-0.7, and TT=38+/-1.6 mm Hg, P=0.029; men: GG=42+/-0.9, GT=41+/-0.8, and TT=39+/-1.5 mm Hg, P=0.47). The only other nominally significant sex-specific association in men but not women was between an intronic polymorphism (rs1800781) and reflected wave amplitude (women: AA=10.4+/-0.4, AG=11.1+/-0.6, and GG=8.9+/-2.2 mm Hg, P=0.50; men: AA=6.1+/-0.3, AG=7.3+/-0.5, and GG=11.3+/-2.3 mm Hg, P=0.014). After adjusting for multiple testing (18 polymorphisms and 5 phenotypes), these nominal associations were no longer significant. The present study was suggestive of modest relations between common genetic variants at the NOS3 locus and arterial stiffness.
authors.affiliation: Cardiovascular Engineering, Inc., Waltham, MA 02453, USA. GaryFMitchell@mindspring.com
authors.forename: Gary F
authors.initials: GF
authors.lastname: Mitchell
authors.forename: Chao-Yu
authors.initials: CY
authors.lastname: Guo
authors.forename: Sekar
authors.initials: S
authors.lastname: Kathiresan
authors.forename: Ramachandran S
authors.initials: RS
authors.lastname: Vasan
authors.forename: Martin G
authors.initials: MG
authors.lastname: Larson
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Vita
authors.forename: Michelle J
authors.initials: MJ
authors.lastname: Keyes
authors.forename: Mitul
authors.initials: M
authors.lastname: Vyas
authors.forename: Christopher
authors.initials: C
authors.lastname: Newton-Cheh
authors.forename: Stacy L
authors.initials: SL
authors.lastname: Musone
authors.forename: Amy L
authors.initials: AL
authors.lastname: Camargo
authors.forename: Jared A
authors.initials: JA
authors.lastname: Drake
authors.forename: Daniel
authors.initials: D
authors.lastname: Levy
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: O'Donnell
authors.forename: Joel N
authors.initials: JN
authors.lastname: Hirschhorn
authors.forename: Emelia J
authors.initials: EJ
authors.lastname: Benjamin
mesh_terms: D000368:Aged; D001158:Arteries / Q000502:physiology*; D050197:Atherosclerosis / Q000235:genetics* / Q000503:physiopathology*; D001794:Blood Pressure / Q000502:physiology*; D002339:Carotid Arteries / Q000503:physiopathology; D015331:Cohort Studies; D004548:Elasticity; D005260:Female; D005263:Femoral Artery / Q000503:physiopathology; D008040:Genetic Linkage; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D020125:Mutation, Missense / Q000235:genetics; D052250:Nitric Oxide Synthase Type III / Q000235:genetics* / Q000502:physiology; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D011673:Pulsatile Flow / Q000235:genetics / Q000502:physiology; D012039:Regional Blood Flow / Q000235:genetics / Q000502:physiology; D012737:Sex Factors
chemical_list: D052250:Nitric Oxide Synthase Type III