An in vitro method for assessing biomaterial-associated platelet activation.
The development of a nonthrombogenic artificial surface for use with indwelling sensors or catheters remains an elusive goal despite decades of ongoing research. In vivo studies are both labor intensive and costly, and are therefore an inefficient way to rapidly screen possible surface materials. The following in vitro model used glass, polyvinyl chloride (PVC), and polypropylene test tubes incubated with 111In-labeled rabbit platelets and illustrated that, despite equivalent platelet count and function, platelet adhesion was greatest on glass (n = 13), with PVC (n = 17) at 67 +/- 8% and polypropylene (n = 13) at 43 +/- 5% when compared with glass. Extrapolating this method by coating test tubes with new, nonthrombogenic materials is a quick and reliable way to screen material before embarking upon more lengthy in vivo animal studies.
LaFayette
Nathan G
NG
Department of General Surgery, University of Michigan, Ann Arbor, Michigan, USA.
Skrzypchak
Amy M
AM
Merz
Scott
S
Bartlett
Robert H
RH
Annich
Gail M
GM
D000818:Animals; D001807:Blood Vessel Prosthesis; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D005898:Glass / Q000737:chemistry; D066298:In Vitro Techniques; D008422:Materials Testing; D015539:Platelet Activation / Q000187:drug effects*; D010973:Platelet Adhesiveness / Q000187:drug effects*; D010976:Platelet Count; D011108:Polymers / Q000494:pharmacology; D011126:Polypropylenes / Q000494:pharmacology; D011143:Polyvinyl Chloride / Q000494:pharmacology; D011817:Rabbits; D015203:Reproducibility of Results
D020099:Coated Materials, Biocompatible; D011108:Polymers; D011126:Polypropylenes; D011143:Polyvinyl Chloride