[Characteristics of carotinoids of methylotrophic bacteria of Methylobacterium genus].
A detailed chromatographic and spectrophotometric analysis of carotinoids in Methylobascterium genus strains: M. extorquens B-3362, M. fujisawaense B-3365, M. mesophilicum B-3352. It has been shown that carotinoids of the studied strains are mainly represented by xanthophylls. The carotinoid common for all three strains and presented in the highest quantity was identified as oscilloxanthin. Carotene found in M. fujisawaense B-3365 and M. mesophilicum B-3352 species was identified as 3,4-didehydrolycopene. Quantitative content of carotenoids was 0.45 mg/g of dry weight for M. fujisawaense B-3365 and M. mesophilicum B-3352 and 0.23 mg/g for M. extorquens B-3362. Interspecies differences were revealed both in qualitative and quantitative content of carotinoids. However, in spite of such differences, carotinoids are similar in these three strains by Rf in thin-layer chromatography and by output time in highly efficient liquid chromatography carotinoids, and make about 80% of the total content of carotinoids.
Konovalova
H M
HM
Shylin
S O
SO
Rokytko
P V
PV
D002338:Carotenoids / Q000302:isolation & purification*; D002855:Chromatography, Thin Layer; D020580:Methylobacterium / Q000737:chemistry* / Q000145:classification / Q000254:growth & development; D013045:Species Specificity; D013053:Spectrophotometry
D002338:Carotenoids