Aggravation of experimental autoimmune neuritis in TNF-alpha receptor 1 deficient mice.
The role of tumor necrosis factor (TNF)-alpha and its receptors in the pathogenesis of experimental autoimmune neuritis (EAN) induced by P0 peptide 180-199 in TNFR1 (p55) deficient (TNFR1-/-) mice was investigated. Compared to wild type EAN mice, TNFR1-/- EAN mice developed significantly more severe clinical signs, in parallel with enhanced numbers of inflammatory infiltrating cells in peripheral nerves and splenic P0-reactive T cell proliferation, as well as increased obviously MHC class II and CCR3 expression on the macrophages in the cauda equina. Our data indicated that TNF-alpha might have anti-inflammatory effect preventing the development of EAN in this mouse model.
Lu
Ming-Ou
MO
Division of Neurodegeneration and Neuroinflammation, Karolinska Institute, Karolinska University Hospital Huddinge, Stockholm, Sweden.
Duan
Rui-Sheng
RS
Quezada
Hernan Concha
HC
Chen
Zhi-Guo
ZG
Mix
Eilhard
E
Jin
Tao
T
Yang
Xin
X
Ljunggren
Hans-Gustaf
HG
Zhu
Jie
J
D000818:Animals; D049109:Cell Proliferation; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005434:Flow Cytometry / Q000379:methods; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D007114:Immunization / Q000379:methods; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018993:Myelin P0 Protein; D009444:Neuritis, Autoimmune, Experimental / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology* / Q000503:physiopathology*; D054397:Receptors, CCR3; D019707:Receptors, Chemokine / Q000378:metabolism; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000172:deficiency*; D012583:Schwann Cells / Q000473:pathology; D012720:Severity of Illness Index; D013936:Thymidine / Q000493:pharmacokinetics
C515351:Ccr3 protein, mouse; D000949:Histocompatibility Antigens Class II; D018993:Myelin P0 Protein; D054397:Receptors, CCR3; D019707:Receptors, Chemokine; D047888:Receptors, Tumor Necrosis Factor, Type I; D015847:Interleukin-4; D007371:Interferon-gamma; D013936:Thymidine