Isolation and characterization of a carbofuran-degrading strain Novosphingobium sp. FND-3.
A gram-negative Novosphingobium sp. strain FND-3 capable of degrading carbofuran was isolated and characterized. The carbofuran-degrading ability of strain FND-3 was investigated under various culture conditions. Strain FND-3 showed a high average carbofuran-degrading rate of 28.6 mg L(-1) h(-1) in mineral salts medium with 100 mg L(-1) carbofuran. GC/MS analysis pointed out the presence of several unknown metabolites. One hydrolyzate was identified as 2-hydroxy-3-(3-methypropan-2-ol) phenol via hydrolysis of carbofuran phenol. The appearance of another metabolite with M(+) of 180 m/z indicated that the hydroxylation of carbofuran occurred at the aromatic ring. One novel degrading product with M(+) of 239 m/z was identified as 2-hydroxy-3-(3-methylpropan-2-ol) benzene-N-methylcarbamate via hydrolyzing at the ether bond of furanyl ring of carbofuran. Strain FND-3 was also able to degrade other N-methylcarbamate pesticides.
Yan
Qiu-Xiang
QX
Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, China.
Hong
Qing
Q
Han
Peng
P
Dong
Xiao-Jun
XJ
Shen
Yu-Jia
YJ
Li
Shun-Peng
SP
D002235:Carbofuran / Q000737:chemistry / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D008401:Gas Chromatography-Mass Spectrometry; D008956:Models, Chemical; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D042301:Sphingomonadaceae / Q000235:genetics / Q000378:metabolism*
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D002235:Carbofuran