title: Determination of aloesin and aloeresin A for the detection of aloe in beverages.
abstract: This work describes a sensitive high-performance liquid chromatography (HPLC) method for the quantification of aloesin and aloeresin A in alcoholic beverages containing aloe as a flavoring agent. The compounds were prepared from Aloe ferox juice. Sephadex LH20 and ion-exchange resin AG1X2 column chromatography were used for aloesin. Aloeresin A was obtained by Sephadex LH20 and silica gel column chromatography followed by purification on Discovery DSC-18 solid-phase extraction tubes. A 98 mg amount of aloesin (>99% purity) and 34 mg of aloeresin A (>98% purity) were recovered from 2.5 g of aloe juice. The HPLC method was validated, and intra- and interday performances were established. In-house validation was carried out by analyzing samples of beverages with and without aloe as a flavoring agent.
authors.affiliation: Research Center for the Characterization and Safe Use of Natural Products, University of Milan, Milan 20129, Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Dell'Agli
authors.forename: Flavio
authors.initials: F
authors.lastname: Giavarini
authors.forename: Patrizia
authors.initials: P
authors.lastname: Ferraboschi
authors.forename: Giovanni
authors.initials: G
authors.lastname: Galli
authors.forename: Enrica
authors.initials: E
authors.lastname: Bosisio
mesh_terms: D000434:Alcoholic Beverages / Q000032:analysis; D000504:Aloe / Q000737:chemistry*; D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002867:Chromones / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis; D005960:Glucosides / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D002867:Chromones; D005960:Glucosides; C521199:aloeresin A; C069868:aloesin