title: Allozyme variation in the camaenid tree snails Amphidromus atricallosus (Gould, 1843) and A. inversus (Müller, 1774).
abstract: We examined allozyme variation in two camaenid tree snails, Amphidromus atricallosus and A. inversus, across two principal regions of Thailand and from Singapore, plus for A. inversus, one site in peninsular Malaysia. Using horizontal starch gel electrophoresis, 13 allozyme loci (11 polymorphic) were screened for A. atricallosus and 18 (5 polymorphic) for A. inversus. Heterozygosity was higher in A. atricallosus (Hexp=0.018-0.201, mean=0.085) than in A. inversus (Hexp=0-0.023, mean= 0.002). Genetic heterogeneity among samples was higher in A. inversus (Fst=0.965) than in A. atricallosus (Fst=0.781). Within A. atricallosus, populations were more differentiated in southern Thailand (Fst=0.551) than in eastern Thailand (Fst=0.144). The high Fst and low Hexp in populations of A. inversus suggest that this species is likely to have experienced a series of strong bottlenecks, perhaps occurring chiefly on offshore continental-shelf islands. The low Fst values of A. atricallosus in eastern Thailand suggest frequent gene flows among populations in this region. The southern and eastern samples of A. atricallosus exhibited fixed allele differences at four loci and great genetic distance (Nei's D=0.485-0.946), suggesting that these two samples may actually represent, or else be evolving into, separate species.
authors.affiliation: Animal Systematic Research Unit, Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Pongpun
authors.initials: P
authors.lastname: Prasankok
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Ota
authors.forename: Mamoru
authors.initials: M
authors.lastname: Toda
authors.forename: Somsak
authors.initials: S
authors.lastname: Panha
mesh_terms: D000818:Animals; D004592:Electrophoresis, Starch Gel; D005787:Gene Frequency; D014644:Genetic Variation; D005838:Genotype; D007527:Isoenzymes / Q000235:genetics*; D008296:Malaysia; D010802:Phylogeny; D011110:Polymorphism, Genetic; D012846:Singapore; D012908:Snails / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D013045:Species Specificity; D013785:Thailand
chemical_list: D007527:Isoenzymes