Use of thermosensitive polymer material on the basis of N-isopropylacrylamide and N-tert-butylacrylamide copolymer in cell technologies.
We developed thermosensitive polymer substrates on the basis of N-isopropylacrylamide and N-tert-butylacrylamide co-polymer and studied their interaction with cultured substrate-dependent mammalian cells. It was shown that these polymers promote cell adhesion and proliferation at a level comparable to polystyrene treated for cell culturing and provide effective cell detachment after lowering culturing temperature below a critical level determined by phase transition temperature in aqueous solutions of polymers. A dependence of phase transition temperature on the ratio between N-isopropylacrylamide and N-tert-butylacrylamide was demonstrated. Differences in the dynamics of cell detachment from the surface of polymer substrates with various proportions between the components were shown.
Selezneva
I I
II
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino.
Gorelov
A V
AV
Rochev
Yu A
YA
D000178:Acrylamides / Q000494:pharmacology*; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D011137:Polystyrenes / Q000494:pharmacology
D000178:Acrylamides; D011137:Polystyrenes; C079805:poly(N-isopropylacrylamide-N-tert-butylacrylamide)copolymer; C067295:N-isopropylacrylamide; C115287:tert-butylacrylamide