title: Bax deficiency reduces infarct size and improves long-term function after myocardial infarction.
abstract: We have previously found that, following myocardial ischemia/reperfusion injury, isolated hearts from bax gene knockout mice [Bax(-/-)] exhibited higher cardioprotection than the wild-type. We here explore the effect of Bax(-/-), following myocardial infarction (MI) in vivo. Homozygotic Bax(-/-) and matched wild-type were studied. Mice underwent surgical ligation of the left anterior descending coronary artery (LAD). The progressive increase in left-ventricular end diastolic diameter, end systolic diameter, in Bax(-/-) was significantly smaller than in Bax(+/+) at 28 d following MI (p < 0.03) as seen by echocardiography. Concomitantly, fractional shortening was higher (35 +/- 4.1% and 27 +/- 2.5%, p < 0.001) and infarct size was smaller in Bax(-/-) compared to the wild-type at 28 days following MI (24 +/- 3.7 % and 37 +/- 3.3%, p < 0.001). Creatine kinase and lactate dehydrogenase release in serum were lower in Bax(-/-) than in Bax(+/+) 24 h following MI. Caspase 3 activity was elevated at 2 h after MI only in the wild-type, but reduced to baseline values at 1 and 28 d post-MI. Bax knockout mice hearts demonstrated reduced infarct size and improved myocardial function following permanent coronary artery occlusion. The Bax gene appears to play a significant role in the post-MI response that should be further investigated.
authors.affiliation: Department of Cardiothoracic Surgery, Felsenstein Medical Research Center, Rabin Medical Center, Petah Tikva, Israel. hochhaus@post.tau.ac.il
authors.forename: E
authors.initials: E
authors.lastname: Hochhauser
authors.forename: Y
authors.initials: Y
authors.lastname: Cheporko
authors.forename: N
authors.initials: N
authors.lastname: Yasovich
authors.forename: L
authors.initials: L
authors.lastname: Pinchas
authors.forename: D
authors.initials: D
authors.lastname: Offen
authors.forename: Y
authors.initials: Y
authors.lastname: Barhum
authors.forename: H
authors.initials: H
authors.lastname: Pannet
authors.forename: A
authors.initials: A
authors.lastname: Tobar
authors.forename: B A
authors.initials: BA
authors.lastname: Vidne
authors.forename: E
authors.initials: E
authors.lastname: Birk
mesh_terms: D000818:Animals; D017209:Apoptosis; D001835:Body Weight; D020169:Caspases / Q000378:metabolism; D004452:Echocardiography / Q000379:methods; D005260:Female; D006579:Heterozygote; D006720:Homozygote; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009203:Myocardial Infarction / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D009206:Myocardium / Q000473:pathology; D013997:Time Factors; D051028:bcl-2-Associated X Protein / Q000378:metabolism*
chemical_list: D051028:bcl-2-Associated X Protein; D020169:Caspases