Effect of aspiration vacuum on the developmental competence of immature human oocytes retrieved using a 20-gauge needle.
In-vitro maturation (IVM) of immature oocytes has been proposed as a potential alternative to conventional IVF treatment following ovarian stimulation. However, the effects of the oocyte retrieval conditions on subsequent development have not been well understood. This study assessed the effects of different aspiration vacuums during oocyte retrieval on the developmental competence of immature oocytes following IVM, IVF and embryo transfer, retrospectively. Immature oocytes were aspirated with 20-gauge needles with a vacuum of 180 or 300 mmHg. Immature oocytes were cultured in IVM medium for 26 h. All mature oocytes were inseminated by intracytoplasmic sperm injection (ICSI). Embryo transfer was carried out 2 or 3 days after ICSI. The percentage of cumulus-cell enclosed oocytes and of transferable embryos per retrieved oocytes in 180 mmHg (69.7% and 23.8%, respectively) were significantly higher (P < 0.01) than those in 300 mmHg (46.2% and 12.8%, respectively). The ongoing pregnancy rate per retrieval cycle in 180 mmHg (30%) was higher (P < 0.01) than that in 300 mmHg (4.3%). The data indicate that lower pressure of vacuum aspiration with a 20-gauge needle improves the developmental competence of immature oocytes following IVM, IVF and embryo transfer.
Hashimoto
Shu
S
IVF Namba Clinic, 1-17-28 Minamihorie, Nishi-ku, Osaka, 550-0015 Japan. hashimoto@ivfnamba.com
Fukuda
Aisaku
A
Murata
Yasutaka
Y
Kikkawa
Miho
M
Oku
Hirotsugu
H
Kanaya
Hiroyuki
H
Sonoda
Momoyo
M
Sugihara
Kengo
K
Murata
Tomoko
T
Nagata
Fumie
F
Nakaoaka
Yoshiharu
Y
Morimoto
Yoshiharu
Y
D000328:Adult; D018929:Cell Culture Techniques / Q000379:methods*; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D009339:Needles; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism; D010062:Ovulation Induction / Q000379:methods*; D011085:Polycystic Ovary Syndrome / Q000628:therapy*; D011247:Pregnancy; D011256:Pregnancy Outcome; D018873:Pregnancy Rate; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*; D014618:Vacuum