title: [Removal of the template molecules from vinblastine-imprinted polymer].
abstract: OBJECTIVE
  To investigate the optimal condition for complete removal of the template molecules from vinblastine (VLB)-imprinted polymer.
  
  
  METHODS
  The prepared polymers were packed into the cartridges of solid-phase extraction column and washed by methanol-glacial acetic acid mixture with different proportions. The contents and recoveries of VLB in the effluents were determined.
  
  
  RESULTS
  Polymer extraction with methanol-glacial acetic acid (9:1, V/V) resulted in VLB recovery of 91.73%, but template bleeding was observed because of incomplete VLB removal. Using methanol-glacial acetic acid (6:4, V/V) as the extraction solvent, the recovery of VLB reached 98.03% with less solvents and extract times. The polymers could selectively adsorb VLB through non-covalent interactions and still exhibited strong affinity for the template molecule but not for the structural analogue vincristine after extraction with methanol-glacial acetic acid (6:4, V/V).
  
  
  CONCLUSIONS
  Methanol-glacial acetic acid (6:4, V/V) is an ideal extract solvent for complete template molecule removal from the polymers, and the processed polymers possess stable capacity of specific recognition and selectivity to the template.
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Jian-yong
authors.initials: JY
authors.lastname: Feng
authors.forename: Quan-hong
authors.initials: QH
authors.lastname: Zhu
authors.forename: Jia-bo
authors.initials: JB
authors.lastname: Luo
mesh_terms: D054802:Molecular Imprinting / Q000379:methods; D010946:Plants, Medicinal / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D014747:Vinblastine / Q000302:isolation & purification*
chemical_list: D011108:Polymers; D012997:Solvents; D014747:Vinblastine