title: Comparison of progestin-based estrus synchronization protocols before fixed-time artificial insemination on pregnancy rate in beef heifers.
abstract: The objective of the experiment was to compare pregnancy rates resulting from fixed-time AI after administration of either 1 of 2 controlled internal drug release (CIDR)-based protocols. Heifers at 3 locations (location 1, n = 78; location 2, n = 61; and location 3, n = 78) were assigned to 1 of 2 treatments within reproductive tract scores (1 = immature to 5 = cycling) by age and BW. Heifers assigned to CIDR Select received a CIDR insert (1.38 g of progesterone) from d 0 to 14 followed by GnRH (100 mug, i.m.) 9 d after CIDR removal (d 23) and PGF2alpha (PG, 25 mg, i.m.) 7 d after GnRH treatment (d 30). Heifers assigned to CO-Synch + CIDR were administered GnRH and received a CIDR insert on d 23 and PG and CIDR removal on d 30. Heifers at location 1 were fitted with a HeatWatch estrus detection system transmitter from the time of PG until 24 d after fixed-time AI to allow for continuous estrus detection. Artificial insemination was performed at predetermined fixed times for heifers in both treatments at 72 or 54 h after PG for the CIDR Select and CO-Synch + CIDR groups, respectively. All heifers were administered GnRH at the time of AI. Blood samples were collected 10 d before and immediately before treatment initiation (d 0) to determine pretreatment estrous cyclicity (progesterone > or = 0.5 ng/mL). At location 1, the estrous response during the synchronized period was greater (P = 0.06; 87 vs. 69%, respectively), and the variance for interval to estrus after PG was reduced among CIDR Select- (P < 0.01) compared with CO-Synch + CIDR-treated heifers. Fixed-time AI pregnancy rates were significantly greater (P = 0.02) after the CIDR Select protocol (62%) compared with the CO-Synch + CIDR protocol (47%). In summary, the CIDR Select protocol resulted in a greater and more synchronous estrous response and significantly greater fixed-time AI pregnancy rates compared with the CO-Synch + CIDR protocol.
authors.affiliation: Division of Animal Science, S132 ASRC, University of Missouri, Columbia 65211, USA.
authors.forename: D C
authors.initials: DC
authors.lastname: Busch
authors.forename: D J
authors.initials: DJ
authors.lastname: Wilson
authors.forename: D J
authors.initials: DJ
authors.lastname: Schafer
authors.forename: N R
authors.initials: NR
authors.lastname: Leitman
authors.forename: J K
authors.initials: JK
authors.lastname: Haden
authors.forename: M R
authors.initials: MR
authors.lastname: Ellersieck
authors.forename: M F
authors.initials: MF
authors.lastname: Smith
authors.forename: D J
authors.initials: DJ
authors.lastname: Patterson
mesh_terms: D000822:Animal Husbandry / Q000379:methods*; D000818:Animals; D002417:Cattle / Q000502:physiology*; D004343:Drug Implants; D004973:Estrus Synchronization / Q000379:methods*; D005260:Female; D005300:Fertility Agents, Female / Q000008:administration & dosage* / Q000494:pharmacology; D007987:Gonadotropin-Releasing Hormone / Q000008:administration & dosage / Q000494:pharmacology; D007315:Insemination, Artificial / Q000662:veterinary; D011247:Pregnancy; D018873:Pregnancy Rate; D011374:Progesterone / Q000097:blood; D011372:Progestins / Q000008:administration & dosage; D013997:Time Factors
chemical_list: D004343:Drug Implants; D005300:Fertility Agents, Female; D011372:Progestins; D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone