Analysis of remote asymmetric somatic hybrids between common wheat and Arabidopsis thaliana.
Callus-derived protoplasts of common wheat (Triticum aestivum L. cv. Hesheng 3) irradiated with ultraviolet light were fused by using the PEG method with cell suspension-derived protoplasts of Arabidopsis thaliana. Regenerated calli and green plants resembling that of wheat were obtained. The hybrid nature of putative calli and plants were confirmed by isozyme, random amplified polymorphic DNA and genomic in situ hybridization (GISH) analyses. GISH results indicated that 1 approximately 3 small chromosome fragments of A. thaliana were found introgression into the terminals of wheat chromosomes, forming highly asymmetric hybrids. Cytoplasmic genome tests did not show any cytoplasmic genetic materials from A. thaliana. However, variations from the normal wheat cytoplasmic genome were found, indicating recombination or rearrangement occurred during the process of somatic hybridization. The chromosome elimination in the asymmetric somatic hybridization of remote phylogenetic relationship was discussed. A miniature inverted-repeat transposable element related sequence was found by chance in the hybrids which might accompany and impact the process of somatic hybridization.
Deng
Jingyao
J
School of Life Sciences, Shandong University, Jinan, 250100 Shandong, China.
Cui
Haifeng
H
Zhi
Daying
D
Zhou
Chuanen
C
Xia
Guangmin
G
D017360:Arabidopsis / Q000166:cytology* / Q000235:genetics*; D002460:Cell Line; D002678:Chimera / Q000235:genetics*; D018745:Genome, Plant; D006824:Hybridization, Genetic; D007621:Karyotyping; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011523:Protoplasts; D046509:Tissue Culture Techniques; D014908:Triticum / Q000166:cytology* / Q000235:genetics*