Rapid DNA hybridization analysis using a PDMS microfluidic sensor and a molecular beacon.
A rapid DNA analysis has been developed based on a fluorescence intensity change of a molecular beacon in a PDMS microfluidic channel. Recently, we reported a new analytical method of DNA hybridization involving a PDMS microfluidic sensor using fluorescence energy transfer (FRET). However, there are some limitations in its application to real DNA samples because the target DNA must be labelled with a suitable fluorescent dye. To resolve this problem, we have developed a new DNA microfluidic sensor using a molecular beacon. By monitoring the change in the restored fluorescence intensity along the channel length, it is possible to rapidly detect any hybridization of the molecular beacon to the target DNA. In this case, the target DNA does not need to be labelled. Our experimental results demonstrate that this microfluidic sensor using a molecular beacon is a promising diagnostic tool for rapid DNA hybridization analysis.
Kim
Sungyong
S
Department of Applied Chemistry, Hanyang University, South Korea.
Chen
Lingxin
L
Lee
Sangyeop
S
Seong
Gi Hun
GH
Choo
Jaebum
J
Lee
Eun Kyu
EK
Oh
Chil-Hwan
CH
Lee
Sanghoon
S
D015342:DNA Probes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D009693:Nucleic Acid Hybridization / Q000379:methods; D009841:Oligonucleotides / Q000737:chemistry*
D015342:DNA Probes; D005456:Fluorescent Dyes; D009841:Oligonucleotides