title: Alternative procedure for charged derivatization to enhance detection responses of steroids in electrospray ionization-MS.
abstract: A derivatization procedure has been examined to enhance the electrospray ionization (ESI)-MS detectabilities of steroids that charged derivatization is not suitable for. The derivatization procedure with 2-hydrazinopyridine or isonicotinoyl azide was very effective for the sensitive detection of di-oxosteroids or di-hydroxysteroids, respectively, and the detection limits of the resulting derivatives were as low as about 2 fmol. The derivatives also provided intense characteristic product ions in the MS-MS, which are expected to be usable for the selected reaction monitoring mode.
authors.affiliation: Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Japan. higashi@p.kanazawa-u.ac.jp
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Higashi
authors.forename: Tadashi
authors.initials: T
authors.lastname: Nishio
authors.forename: Noriko
authors.initials: N
authors.lastname: Hayashi
authors.forename: Kazutake
authors.initials: K
authors.lastname: Shimada
mesh_terms: D002853:Chromatography, Liquid; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013256:Steroids / Q000032:analysis*; D053719:Tandem Mass Spectrometry
chemical_list: D013256:Steroids