Assessing hERG channel inhibition using PatchXpress.
PatchXpress, an automated 16-channel parallel patch clamp system, was used to determine inhibition of human ether-a-go-go related gene (hERG) potassium channels by known blockers. A monoclonal cell line stably expressing hERG potassium channels was generated in CHO-K1 cells. Results were compared to conventional patch clamp experiments using similar voltage protocols and solutions. Success rates were evaluated for cell recordings under a variety of conditions, including Accumax versus trypsin treatment to harvest cells, single versus double compound additions, and polystyrene versus glass-coated compound plates. Finally, polystyrene versus glass-coated compound plates were evaluated, and the authors found that for some compounds (but not all), preparation of compound samples in glass-coated plates resulted in inhibition that more closely matched data obtained by conventional experiments.
Ly
Justin Q
JQ
Roche Palo Alto, Department of Non-Clinical Drug Safety, 3431 Hillview Avenue, Palo Alto, CA 94304, USA.
Shyy
Gordon
G
Misner
Dinah L
DL
D000818:Animals; D001331:Automation; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D051638:Ether-A-Go-Go Potassium Channels / Q000037:antagonists & inhibitors* / Q000378:metabolism; D006801:Humans; D018408:Patch-Clamp Techniques / Q000295:instrumentation* / Q000379:methods*; D015203:Reproducibility of Results; D012984:Software
D051638:Ether-A-Go-Go Potassium Channels