Low- and high-grade non-invasive gastric neoplasia (formerly dysplasia): cytological differentiation (gastro-entero-pancreatic antigens) in association with p53 pattern expression.
OBJECTIVE
In order to better define the evolutive potentiality of non-invasive neoplasia (formerly dysplasia) a study of the cytological differentiation and of the behavior of p53 in relation to the clinical progress has been performed.


METHODS
Gastro-entero-pancreatic antigens, p53 and Ki-67 expression were evaluated in 120 cases of epithelial gastric dysplasia: 70 cases of low-grade dysplasia (LGD) and 50 cases of high-grade dysplasia (HGD). For the cytological study four antigens were studied: two of them gastric (pepsinogen C, gastric foveolar M1), one enteric (CAR-5) and one pancreatic (DU-PAN-2). Routinely processed tissue sections of a colon carcinoma known to contain mutant p53 were used as positive controls for p53 immunohistochemistry. For Ki-67 immunohistochemistry, routinely processed tissue sections of normal lymph node and tonsil were used as staining controls.


RESULTS
The study of the coexpression of the gastro-entero-pancreatic antigens showed how all cases of non-invasive neoplasia associated with or progressed to gastric carcinoma (GC) were characterized by entero-pancreatic markers and, in particular, in case of LGD p53 expression positivity was evidenced in 66.6% of cases. Ki-67 hyperproliferation is present in 100% of cases.


CONCLUSIONS
The cytological study, only if confirmed by wider casuistries, could represent further information in order to better define the follow-up and the therapeutical decisions in case of non-invasive gastric neoplasia therefore, the immunophenotypic study in association with p53 could lead to more personalized therapeutical choices.
Testino
Gianni
G
Unit of Hepato-Gastroenterology, Department of Organ Transplantation San Martino Hospital, Genova, Italy. testinogia@tiscalinet.it
Cornaggia
Matteo
M
Ferrando
Valter
V
D000951:Antigens, Neoplasm / Q000378:metabolism*; D002454:Cell Differentiation; D003430:Cross-Sectional Studies; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D020115:Pepsinogen C / Q000378:metabolism; D051603:Recoverin / Q000378:metabolism; D012189:Retrospective Studies; D013274:Stomach Neoplasms / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
D000951:Antigens, Neoplasm; C042317:DU-PAN-2 antigen, human; D019394:Ki-67 Antigen; C496731:RCVRN protein, human; D016159:Tumor Suppressor Protein p53; D051603:Recoverin; D020115:Pepsinogen C