title: Interaction of the selectin ligand PSGL-1 with chemokines CCL21 and CCL19 facilitates efficient homing of T cells to secondary lymphoid organs.
abstract: P-selectin glycoprotein ligand 1 (PSGL-1) is central to the trafficking of immune effector cells to areas of inflammation through direct interactions with P-selectin, E-selectin and L-selectin. Here we show that PSGL-1 was also required for efficient homing of resting T cells to secondary lymphoid organs but functioned independently of selectin binding. PSGL-1 mediated an enhanced chemotactic T cell response to the secondary lymphoid organ chemokines CCL21 and CCL19 but not to CXCL12 or to inflammatory chemokines. Our data show involvement of PSGL-1 in facilitating the entry of T cells into secondary lymphoid organs, thereby demonstrating the bifunctional nature of this molecule.
authors.affiliation: The Biomedical Research Centre, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada.
authors.forename: Krystle M
authors.initials: KM
authors.lastname: Veerman
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Williams
authors.forename: Kenji
authors.initials: K
authors.lastname: Uchimura
authors.forename: Mark S
authors.initials: MS
authors.lastname: Singer
authors.forename: Jasmeen S
authors.initials: JS
authors.lastname: Merzaban
authors.forename: Silvia
authors.initials: S
authors.lastname: Naus
authors.forename: Douglas A
authors.initials: DA
authors.lastname: Carlow
authors.forename: Philip
authors.initials: P
authors.lastname: Owen
authors.forename: Jesús
authors.initials: J
authors.lastname: Rivera-Nieves
authors.forename: Steven D
authors.initials: SD
authors.lastname: Rosen
authors.forename: Hermann J
authors.initials: HJ
authors.lastname: Ziltener
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000235:genetics / Q000276:immunology; D054415:Chemokine CCL19; D054421:Chemokine CCL21; D019742:Chemokines, CC / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D006706:Homeostasis / Q000502:physiology*; D008221:Lymphoid Tissue / Q000166:cytology* / Q000276:immunology; D008562:Membrane Glycoproteins / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D013601:T-Lymphocytes / Q000502:physiology*
chemical_list: C515461:Ccl19 protein, mouse; C515479:Ccl21c protein, mouse; D054415:Chemokine CCL19; D054421:Chemokine CCL21; D019742:Chemokines, CC; D008562:Membrane Glycoproteins; C084882:P-selectin ligand protein