title: From the one-bead-one-compound concept to one-bead-one-reactor.
abstract: The one-bead-one-compound method gives access to millions of compounds that can be screened directly on the bead. Although characterization techniques are increasingly potent and reliable, problems can still be encountered in deciphering the sequence of the active compound because of sensitiveness or manipulation of the bead. ChemMatrix, a totally PEG-based resin, has resolved the synthesis of peptides of outstanding difficulty. Like other PEG-based resins, it permits on-bead screening because of its compatibility in aqueous media and has the further advantage of having a high loading, comparable to polystyrene resins. ChemMatrix beads previously swelled in water can be nicely divided into four parts that can be characterized using different analytical techniques or just stored for safety or for further testing. The four bead parts show high homogeneity and can thus be considered to be replicas.
authors.affiliation: Institute for Research in Biomedicine, Barcelona Science Park, Josep Samitier 1-5, 08028-Barcelona, Spain.
authors.forename: Mariela M
authors.initials: MM
authors.lastname: Marani
authors.forename: Marta
authors.initials: M
authors.lastname: Paradís-Bas
authors.forename: Judit
authors.initials: J
authors.lastname: Tulla-Puche
authors.forename: Simón
authors.initials: S
authors.lastname: Côté
authors.forename: Silvia A
authors.initials: SA
authors.lastname: Camperi
authors.forename: Osvaldo
authors.initials: O
authors.lastname: Cascone
authors.forename: Fernando
authors.initials: F
authors.lastname: Albericio
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D020650:Combinatorial Chemistry Techniques / Q000379:methods; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D012117:Resins, Synthetic / Q000737:chemistry*; D012680:Sensitivity and Specificity; D014867:Water / Q000737:chemistry
chemical_list: D010455:Peptides; D012117:Resins, Synthetic; D014867:Water; D011092:Polyethylene Glycols