title: Dermaseptins from Phyllomedusa oreades and Phyllomedusa distincta: liposomes fusion and/or lysis investigated by fluorescence and atomic force microscopy.
abstract: Three dermaseptins, DS 01, DD K, and DD L, were compared with respect to their structural features and interactions with liposomes. Circular dichroic spectra at alcohols of different chain lengths revealed that DS 01 has the higher helicogenic potential in hydrophobic media. Binding of DS 01, DD K, and DD L to liposomes induced significant blue shifts of the emission spectra of the single tryptophan located at position 3 of all sequences indicating association of the peptides with bilayers. Kinetics evaluation of atomic force microscopy images evidenced the strong fusogenic activity of DS 01 whereas DD K and DD L showed increased lytic activities.
authors.affiliation: Laboratório de Morfologia e Morfogênese, Departamento de Genética e Morfologia, Instituto de Biologia, Universidade de Brasília, Brasília, DF, 70910-900, Brazil; Laboratório de Espectrometria de Massa, Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)-Recursos Genéticos e Biotecnologia, Estação Parque Biológico, Final W5, Asa Norte, Brasília, DF, 70770-900, Brazil. Electronic address: paulinol@cenargen.embrapa.br.
authors.forename: Luciano P
authors.initials: LP
authors.lastname: Silva
authors.affiliation: Laboratório de Espectrometria de Massa, Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)-Recursos Genéticos e Biotecnologia, Estação Parque Biológico, Final W5, Asa Norte, Brasília, DF, 70770-900, Brazil; Campus Ministro Reis Velloso, Universidade Federal do Piauí-UFPI, Parnaíba, Piauí, 64202-020, PI, Brazil.
authors.forename: José Roberto S A
authors.initials: JRSA
authors.lastname: Leite
authors.affiliation: Laboratório de Espectrometria de Massa, Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)-Recursos Genéticos e Biotecnologia, Estação Parque Biológico, Final W5, Asa Norte, Brasília, DF, 70770-900, Brazil.
authors.forename: Guilherme D
authors.initials: GD
authors.lastname: Brand
authors.affiliation: Grupo de Biofísica Molecular-Centro de Biologia Molecular Estrutural, Laboratório Nacional de Luz Síncrotron (LNLS), Campinas, SP, Brazil.
authors.forename: Willian B
authors.initials: WB
authors.lastname: Regis
authors.affiliation: FFCLRP-USP, Universidade de São Paulo, Ribeirão Preto, SP, 14040-901, Brazil.
authors.forename: Antonio C
authors.initials: AC
authors.lastname: Tedesco
authors.affiliation: Laboratório de Morfologia e Morfogênese, Departamento de Genética e Morfologia, Instituto de Biologia, Universidade de Brasília, Brasília, DF, 70910-900, Brazil.
authors.forename: Ricardo B
authors.initials: RB
authors.lastname: Azevedo
authors.affiliation: Laboratório de Biofísica, Departamento de Biologia Celular, Instituto de Biologia, Universidade de Brasília, 70910-900 Brazil.
authors.forename: Sonia M
authors.initials: SM
authors.lastname: Freitas
authors.affiliation: Laboratório de Espectrometria de Massa, Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)-Recursos Genéticos e Biotecnologia, Estação Parque Biológico, Final W5, Asa Norte, Brasília, DF, 70770-900, Brazil. Electronic address: cbloch@cenargen.embrapa.br.
authors.forename: Carlos
authors.initials: C
authors.lastname: Bloch
mesh_terms: D000438:Alcohols / Q000494:pharmacology; D000595:Amino Acid Sequence; D029845:Amphibian Proteins / Q000737:chemistry* / Q000378:metabolism*; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry* / Q000378:metabolism*; D001001:Anura; D002942:Circular Dichroism; D007700:Kinetics; D008081:Liposomes / Q000378:metabolism*; D008561:Membrane Fusion / Q000502:physiology*; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D011485:Protein Binding; D017433:Protein Structure, Secondary / Q000187:drug effects; D013329:Structure-Activity Relationship
chemical_list: D000438:Alcohols; D029845:Amphibian Proteins; D023181:Antimicrobial Cationic Peptides; D008081:Liposomes; C527843:dermadistinctin K, Phyllomedusa distincta; C070905:dermaseptin