title: Phylotype diversity of deep-sea hydrothermal vent prokaryotes trapped by 0.2- and 0.1-microm-pore-size filters.
abstract: Eleven 16S rRNA gene clone libraries including 34 archaeal and 72 bacterial phylotypes were constructed from total 708 clones of hydrothermal vent prokaryotes trapped by 0.2- and 0.1-microm-pore-size filters. Crenarchaeota and Proteobacteria phylotypes dominated the archaeal and bacterial populations, respectively. Novel unaffiliated phylotypes occurred only in the 0.1-microm-trapped populations.
authors.affiliation: Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima 739-8528, Japan. takn@hiroshima-u.ac.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Naganuma
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Miyoshi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kimura
mesh_terms: D001105:Archaea / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D044822:Biodiversity; D019615:Crenarchaeota / Q000145:classification; D019641:DNA, Archaeal / Q000032:analysis; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005374:Filtration / Q000295:instrumentation*; D015723:Gene Library; D007564:Japan; D008851:Micropore Filters; D010802:Phylogeny; D016062:Porosity; D011387:Prokaryotic Cells; D012336:RNA, Ribosomal, 16S; D021521:Ribotyping; D012623:Seawater / Q000382:microbiology*; D014871:Water Microbiology
chemical_list: D019641:DNA, Archaeal; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S