title: HLA homozygosity does not adversely affect measles vaccine-induced cytokine responses.
abstract: The association between HLA homozygosity and measles-specific Th(1) (IFN-gamma, IL-2 and IL-12p40) and Th(2) (IL-4 and IL-10) cytokine responses were assessed in a group of 339 healthy schoolchildren 12-18 years of age previously immunized with two doses of live-attenuated measles virus vaccine. No associations were observed between class I HLA homozygosity and measles-specific cytokine levels. Children who were homozygous at the class II DRB1, DQA1, DPA1 and DPB1 loci had higher median IFN-gamma secretion levels compared with children who were heterozygous for DRB1 (77.7 vs. 39.5 pg/ml, p=0.05), DQA1 (60.9 vs. 36.6 pg/ml, p=0.03), DPA1 (46.1 vs. 27.1 pg/ml, p=0.01) and DPB1 (61.5 vs. 36.0 pg/ml, p=0.01) loci, respectively. Homozygosity at increasing numbers of HLA loci ( >or=4) was associated with increased IFN-gamma secretion levels (test for trend p-value=0.01). Our results suggest that HLA homozygosity showed no disadvantage for measles-specific cytokine responses and instead was associated with increased IFN-gamma levels.
authors.affiliation: Mayo Clinic Vaccine Research Group, Mayo Clinic College of Medicine, Rochester, MN 55905, USA. ovsyannikova.inna@mayo.edu <ovsyannikova.inna@mayo.edu>
authors.forename: Inna G
authors.initials: IG
authors.lastname: Ovsyannikova
authors.forename: Robert M
authors.initials: RM
authors.lastname: Jacobson
authors.forename: Neelam
authors.initials: N
authors.lastname: Dhiman
authors.forename: Robert A
authors.initials: RA
authors.lastname: Vierkant
authors.forename: V Shane
authors.initials: VS
authors.lastname: Pankratz
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Poland
mesh_terms: D000293:Adolescent; D002648:Child; D016207:Cytokines / Q000096:biosynthesis* / Q000097:blood; D005260:Female; D005805:Genes, MHC Class I; D005802:Genes, MHC Class II; D006680:HLA Antigens / Q000235:genetics*; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D053762:Interleukin-12 Subunit p40 / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D008297:Male; D008458:Measles Vaccine / Q000008:administration & dosage / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
chemical_list: D016207:Cytokines; D006680:HLA Antigens; C508609:IL10 protein, human; C500879:IL12B protein, human; C508594:IL2 protein, human; C508598:IL4 protein, human; D053762:Interleukin-12 Subunit p40; D007376:Interleukin-2; D008458:Measles Vaccine; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma