[Effect of 5 drugs with different antitumor mechanism on tumor-formation potential of rat hepatocarcinoma cells and mesenchymal stem cells].
OBJECTIVE
To study the association of drug sensitivity of rat hepatocarcinoma cells and bone marrow mesenchymal stem cells (MSCs) to 5 antitumor drug with different antitumor mechanisms with tumorigenicity of the hepatocarcinoma cells in nude mice.


METHODS
Primary liver carcinoma was induced with diethylnitrosamine in rats, and the tumor cells and MSCs were obtained from 10 of the rats. The inhibition ratio of the hepatocarcinoma cells and MSCs following treatment with the 5 drugs were measured by MTT assay. The weight of the tumor in nude mice resulting from the injection of the isolated tumor cells treated with the 5 anticancer drugs was measured 6 weeks after implantation. For each anticancer drug, the difference in the inhibition ratio of the anticancer drugs against the hepatocarcinoma cells and MSCs, and he correlation of the inhibition ratio of the anticancer drugs with implanted tumor weight were analyzed.


RESULTS
No correlation was found between the inhibition ratio of the 5 anticancer drugs against the hepatocarcinoma cells and the tumor weight of nude mice, but a significant negative correlation was identified between the inhibition ratio of the MSCs and the tumor weight.


CONCLUSIONS
MSCs have similar drug resistance mechanism to the tumor stem cells. The inhibition ratio of the anticancer drugs against the MSCs can help evaluate the invasion potential of hepatocarcinoma cells.
Zhou
Yuan
Y
Oncology Center, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Luo
Min
M
Zhang
Jing
J
Zhang
Wen-qing
WQ
Zhang
Ji-ren
JR
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D008114:Liver Neoplasms, Experimental / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D014411:Neoplastic Stem Cells / Q000187:drug effects* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays / Q000379:methods
D000970:Antineoplastic Agents