title: Multicommuted fluorometric multiparameter sensor for simultaneous determination of naproxen and salicylic acid in biological fluids.
abstract: A combined approach based on solid-phase optosensing and multicommutation principles has been applied to develop a method for the simultaneous analysis of two pharmaceuticals (naproxen and salicylic acid) in biological fluids. The multicommuted flow-through optosensor was based on direct native fluorescence measurements of both analgesics using a non-polar sorbent (C18 silica gel) as a solid sensing zone. The flow system was controlled by Java-written home-made software and designed using three-way solenoid valves for an independent automated manipulation of sample and carrier solutions. Using an optimized sampling time, the method was calibrated in the range of 1 - 25 and 5 - 200 ng mL(-1). The obtained detection limits were 0.3 and 1.3 ng mL(-1) for naproxen and salicylic acid, respectively, with RSD (%) values of better than 2% for both analytes. The proposed methodology was successfully applied to urine, serum and pharmaceutical preparations. Recovery percentages ranging from 96.1 to 104% were obtained for both analytes.
authors.affiliation: Department of Physical and Analytical Chemistry, Faculty of Experimental Sciences, University of Jaén, Spain.
authors.forename: Juan F
authors.initials: JF
authors.lastname: García-Reyes
authors.forename: Pilar
authors.initials: P
authors.lastname: Ortega-Barrales
authors.forename: Antonio
authors.initials: A
authors.lastname: Molina-Díaz
mesh_terms: D000890:Anti-Infective Agents / Q000097:blood* / Q000652:urine; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood* / Q000652:urine; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D005453:Fluorescence; D006801:Humans; D009288:Naproxen / Q000097:blood* / Q000652:urine; D020156:Salicylic Acid / Q000097:blood* / Q000652:urine; D058428:Silica Gel; D012822:Silicon Dioxide / Q000737:chemistry; D012984:Software
chemical_list: D000890:Anti-Infective Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D009288:Naproxen; D058428:Silica Gel; D012822:Silicon Dioxide; D020156:Salicylic Acid