title: Neural defects and cardiac arrhythmia in fish larvae following embryonic exposure to 2,2',4,4'-tetrabromodiphenyl ether (PBDE 47).
abstract: Polybrominated diphenyl ethers (PBDEs) are added to plastics, polyurethane foam, and textiles as a flame retardant. While PBDEs play a key role in reducing loss of human life and property from fires, these flame retardants have become pervasive organic contaminants in the environment and in the tissues of fish, birds, marine mammals, and humans. Levels of PBDEs in wildlife and humans continue to rise, raising concerns about potential ecological and health risks associated with exposure to these chemicals. Nevertheless, there is little currently known about the toxicological effects of PBDE exposure. Here, we examined the developmental toxicity of the PBDE congener 2,2',4,4'-tetrabromodiphenyl ether (PBDE 47) using the zebrafish (Danio rerio) as an ontogenetic model. Zebrafish embryos were exposed continuously to dissolved phase PBDE 47 (100-5000 microg/l) beginning 3-5 h post-fertilization (hpf). Fish treated with the highest concentrations of PBDE 47 delayed hatching, had reduced growth post-hatching, and displayed an abnormal dorsal curvature of the body with flexion at the hindbrain. By 96h post-fertilization larvae exposed to PBDE 47 had significant tachycardia, which progressed into atrioventricular block arrhythmias. Microinjection of fluorescent dye into the hindbrain ventricle revealed that cerebrospinal fluid in the neural tube and brain ventricles flowed more slowly in fish larvae exposed to PBDE 47, a likely etiology for the dorsal curvature. Similar, though much less pronounced, developmental toxicity also occurred in larvae exposed to PBDE 47 only for a 20h period during early embryogenesis (3-23 hpf), suggesting that PBDEs incorporated in lipid of the egg are bioavailable and cause toxicity later in life. Taken together, this work indicates that exposure to PBDE 47 can cause morphological abnormalities, impair cardiovascular function and cerebrospinal fluid flow, and provides a tractable starting point for using the zebrafish model to explore molecular mechanisms of PBDE toxicity.
authors.affiliation: NOAA Fisheries, Northwest Fisheries Science Center, 2725 Montlake Boulevard East, Seattle, WA 98112, USA. sean.lema@noaa.gov
authors.forename: Sean C
authors.initials: SC
authors.lastname: Lema
authors.forename: Irvin R
authors.initials: IR
authors.lastname: Schultz
authors.forename: Nathaniel L
authors.initials: NL
authors.lastname: Scholz
authors.forename: John P
authors.initials: JP
authors.lastname: Incardona
authors.forename: Penny
authors.initials: P
authors.lastname: Swanson
mesh_terms: D000818:Animals; D002490:Central Nervous System / Q000187:drug effects* / Q000254:growth & development / Q000503:physiopathology; D002555:Cerebrospinal Fluid / Q000187:drug effects / Q000502:physiology; D004625:Embryo, Nonmammalian / Q000187:drug effects*; D005260:Female; D055768:Halogenated Diphenyl Ethers; D006321:Heart / Q000187:drug effects* / Q000254:growth & development / Q000503:physiopathology; D006842:Hydrocarbons, Brominated / Q000032:analysis / Q000633:toxicity*; D007814:Larva / Q000737:chemistry / Q000187:drug effects; D008297:Male; D010647:Phenyl Ethers / Q000032:analysis / Q000633:toxicity*; D016019:Survival Analysis; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*; D015027:Zebrafish / Q000196:embryology / Q000254:growth & development*
chemical_list: D055768:Halogenated Diphenyl Ethers; D006842:Hydrocarbons, Brominated; D010647:Phenyl Ethers; D014874:Water Pollutants, Chemical; C511295:2,2',4,4'-tetrabromodiphenyl ether