title: Enrichment methodology to increase the positivity of cultures from body fluids.
abstract: Isolation and identification of etiological agents found in body fluids can be of critical importance for the recovery of patients suffering from potentially-severe infections, which are often followed by serious sequels. Eighty-two samples of different body fluids were analyzed using two different methods: (1) the conventional culture method (agar plating) and (2) the enrichment culture technique, using the Bact/Alert blood culture bottle. The number of positive cultures increased on average from 9.7% to 23.1% with the enrichment culture technique. Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus were the most frequently isolated bacteria. The enrichment method could provide a more accurate means the identifying etiological agents.
authors.affiliation: Clinical Laboratory, Curitiba General Hospital, Curitiba, PR, Brazil.
authors.forename: Alessandra Valle
authors.initials: AV
authors.lastname: Daur
authors.forename: Francisco
authors.initials: F
authors.lastname: Klimak
authors.forename: Laura Lúcia
authors.initials: LL
authors.lastname: Cogo
authors.forename: Gislene Diógenes
authors.initials: GD
authors.lastname: Botão
authors.forename: Cristina Leise Bastos
authors.initials: CL
authors.lastname: Monteiro
authors.forename: Libera Maria
authors.initials: LM
authors.lastname: Dalla Costa
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D001826:Body Fluids / Q000382:microbiology*; D003470:Culture Media; D006090:Gram-Negative Bacteria / Q000302:isolation & purification*; D006094:Gram-Positive Bacteria / Q000302:isolation & purification*; D006801:Humans
chemical_list: D003470:Culture Media