title: Effects of eccentric exercise on NF-kappaB activation in blood mononuclear cells.
abstract: OBJECTIVE
  Changes in nuclear factor kappaB (NF-kappaB) activation induced in peripheral blood mononuclear cells (PBMC) by acute eccentric exercise and by submaximal eccentric training were investigated.
  
  
  METHODS
  Eleven subjects carried out two bouts of eccentric exercise separated by 6 wk of training.
  
  
  RESULTS
  Soreness, vertical jump height, and plasma creatine kinase were significantly modified after the first bout. NF-kappaB activation, p50 and p65, phospho-IkappaBalpha and phospho-IKK protein level, and Mn-SOD expression increased in PBMC, whereas IkappaBalpha protein level was significantly reduced. Changes were significantly attenuated after the second exercise bout. An additional group of nine subjects carried out the two bouts of exercise without training. Effects on NF-kappaB activation were similar after the second bout compared with the first, despite a reduction in markers of muscle injury (repeated bout effect).
  
  
  CONCLUSIONS
  Training significantly attenuates the NF-kappaB-dependent pathway changes induced in PBMC by eccentric exercise, with no contribution from the repeated bout effect.
authors.affiliation: Institute of Biomedicine, University of León, León, Spain.
authors.forename: David
authors.initials: D
authors.lastname: García-López
authors.forename: María J
authors.initials: MJ
authors.lastname: Cuevas
authors.forename: Mar
authors.initials: M
authors.lastname: Almar
authors.forename: Elena
authors.initials: E
authors.lastname: Lima
authors.forename: José A
authors.initials: JA
authors.lastname: De Paz
authors.forename: Javier
authors.initials: J
authors.lastname: González-Gallego
mesh_terms: D000328:Adult; D015444:Exercise / Q000502:physiology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D016328:NF-kappa B / Q000032:analysis / Q000097:blood / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology; D013030:Spain
chemical_list: D016328:NF-kappa B