Autoantibodies against glial fibrillary acidic protein (GFAP) in cerebrospinal fluids from Pug dogs with necrotizing meningoencephalitis.
Cerebrospinal fluids (CSFs) from 9 Pug dogs with necrotizing meningoencephalitis (NME: Pug dog encephalitis) were examined to identify the antigens for anti-astrocyte autoantibodies. Each CSF exhibited a positive reaction to the cytoplasm of cultured canine astrocytes by an indirect fluorescent antibody test. In an immunoblotting analysis on normal canine brain proteins, eight of 9 CSFs showed a common band of 52 kDa, corresponding to glial fibrillary acidic protein (GFAP), and all of 9 CSFs reacted with purified bovine GFAP. From these results, GFAP is one of the common autoantigens in Pug dogs with NME. On the other hand, the reactivity of CSFs to chymotrypsin-digested bovine GFAP fragments were variable among dogs, indicating that the antibodies in the CSFs recognized different epitopes on GFAP.
Shibuya
Masahiro
M
Department of Veterinary Clinical Pathobiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
Matsuki
Naoaki
N
Fujiwara
Kaori
K
Imajoh-Ohmi
Shinobu
S
Fukuda
Hiroyuki
H
Pham
Ngoc T
NT
Tamahara
Satoshi
S
Ono
Kenichiro
K
D000818:Animals; D001253:Astrocytes / Q000276:immunology; D001323:Autoantibodies / Q000134:cerebrospinal fluid* / Q000276:immunology; D004283:Dog Diseases / Q000134:cerebrospinal fluid* / Q000276:immunology*; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D000939:Epitopes / Q000235:genetics; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics / Q000276:immunology*; D015151:Immunoblotting / Q000662:veterinary; D013058:Mass Spectrometry / Q000662:veterinary; D008590:Meningoencephalitis / Q000134:cerebrospinal fluid / Q000276:immunology / Q000662:veterinary*
D001323:Autoantibodies; D000939:Epitopes; D005904:Glial Fibrillary Acidic Protein