c-Myb immunoreactivity, protein and mRNA levels significantly increase in the aged hippocampus proper in gerbils.
Myb genes are a family of transcription factors and have been implicated in the control of the proliferation and differentiation of normal and transformed cells. c-Myb is the best characterized member of the myb family. In the present study, we investigated age-dependent changes of c-myb immunoreactivity, its protein and mRNA level in the hippocampus proper (CA1-3 regions) at various age stages in gerbils. In the postnatal month 1 (PM 1) group, c-myb immunoreactivity was detected in non-pyramidal neurons of the strata oriens and radiatum as well as in pyramidal neurons of the stratum pyramidale. At PM 3, c-myb immunoreactivity and its protein level were similar to those at PM 1. Thereafter, c-myb immunoreactivity and its protein level were increased with time. In the PM 24 group, c-myb immunoreactivity, its protein and mRNA levels were highest. These results suggest that the significant increase of c-myb immunoreactivity, protein and mRNA levels in the aged hippocampus may be associated with neuronal aging.
Hwang
In Koo
IK
Department of Anatomy and Neurobiology, College of Medicine, Hallym University, Chuncheon, South Korea.
Moon
Seung Myung
SM
Yoo
Ki-Yeon
KY
Li
Hua
H
Kwon
Heum Dai
HD
Hwang
Hyung Sik
HS
Choi
Sun Kil
SK
Lee
Bong-Hee
BH
Kim
Jong Dai
JD
Won
Moo Ho
MH
D000375:Aging / Q000502:physiology*; D000818:Animals; D015153:Blotting, Western; D020570:Genes, myb / Q000502:physiology*; D005849:Gerbillinae; D006624:Hippocampus / Q000254:growth & development* / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D020598:Proto-Oncogene Proteins c-myb / Q000096:biosynthesis / Q000378:metabolism*; D017966:Pyramidal Cells / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D020598:Proto-Oncogene Proteins c-myb; D012333:RNA, Messenger