Evidence for non-chemical, non-electrical intercellular signaling in intestinal epithelial cells.
Synchrony between mechanically separated biological systems is well known. We posed the question: can cells induce synchronous behavior in neighboring cells which are mechanically separated and which cannot communicate via chemical or electrical mechanisms. Caco-2 cell cultures were divided into three groups. "Inducer" cells were exposed to H(2)O(2). "Detector" cells were placed in separate containers near the inducer cells but were not exposed to H(2)O(2). Control cells were exposed to fresh media and were kept in a distant laboratory area. Samples were measured for total protein concentration, NFkappaB activation and structural changes, 10, 30 and 60 min after exposure respectively. Exposing inducer cells to H(2)O(2) resulted in a significant reduction in total protein content (-50%), an increase in nuclear NFkappaB activation (+38%), and structural damage (56%) compared to controls. There was a similar reduction in total protein content (-48%), increase in the nuclear fraction of NFkappaB (+35%) and structural damage (25%) in detector cells. These findings provide evidence in support of a non-chemical, non-electrical communication. This signaling system possibly plays a role in synchronous, stimulus-appropriate cell responses to noxious stimuli and may explain a number of cellular behaviors that are hard to explain based only on conventional cell signaling systems.
Farhadi
Ashkan
A
Section of Gastroenterology and Nutrition, Rush University Medical Center, Chicago, IL 60612, USA. Ashkan_farhadi@rush.edu
Forsyth
Christopher
C
Banan
Ali
A
Shaikh
Maliha
M
Engen
Phillip
P
Fields
Jeremy Z
JZ
Keshavarzian
Ali
A
D000199:Actins / Q000378:metabolism; D000818:Animals; D018938:Caco-2 Cells; D002417:Cattle; D002450:Cell Communication; D003599:Cytoskeleton / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D007422:Intestines / Q000166:cytology*; D008856:Microscopy, Fluorescence; D016328:NF-kappa B / Q000378:metabolism; D016877:Oxidants / Q000378:metabolism / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects; D013053:Spectrophotometry; D019108:Tight Junctions; D015854:Up-Regulation / Q000187:drug effects
D000199:Actins; D016328:NF-kappa B; D016877:Oxidants; D006861:Hydrogen Peroxide