[Preparation of the monoclonal antibodies against human BORIS and the expression pattern of BORIS in normal and diseased human mammary tissues].
OBJECTIVE
To prepare monoclonal antibodies(mAb) to BORIS antigen and analyze its expression pattern in breast cancer, benign breast disease and normal breast tissues.


METHODS
Female BALB/c mice were immunized with recombination human BORIS protein. Hybridoma cell lines were established by hybridoma technique. BORIS antigen was detected by immunohistochemical (ICH) staining.


RESULTS
Four clones of hybridomas stably secereting mAb against human BORIS were obtained. mAb FMMU-BORIS13 was selected to be employed in ICH. Positive staining was localized in the nuclei of the spermatocytes and spermatogoniums, which was consistent with the typical expression pattern of CT antigen. BORIS antigen was detected in 85%(94/110) of breast cancer, 95%(21/22) of benign breast diseases and 6 normal breast tissues(surrouding tumor free breast tissue).


CONCLUSIONS
Four clones of anti-BORIS mAb have been successfully prepared. The expression of BORIS antigen is much stronger in breast cancer than that in benign breast disease and normal breast tissues, which indicate that BORIS may be involved in the pathogenesis of the breast cancer.
Zhou
Xing-Chun
XC
Department of Immunology, Fourth Military Medical University, Xi'an 710032, China.
Song
Chao-Jun
CJ
Wang
Xin
X
Tian
Ying
Y
Jin
Bo-Quan
BQ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D001943:Breast Neoplasms / Q000503:physiopathology*; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000276:immunology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007150:Immunohistochemistry; D042361:Mammary Glands, Human / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; C456540:CTCFL protein, human; D004268:DNA-Binding Proteins