[Study of the expression of exogenous augmenter of liver regeneration recombinant plasmid in liver tissue of rat].
OBJECTIVE
To observe the expression of exogenous ALR recombinant plasmid in liver tissue of rats suffered from immune hepatic fibrosis.


METHODS
Immune hepatic fibrosis model of rats were made by using porcine serum, which were treated by pcDNA3-ALR recombinant plasmid. The expression of ALR proteins in liver tissue were detected by immunohistochemistry staining and Western blotting and the expression of ALR mRNA were detected by RT-PCR.


RESULTS
ALR were expressed in liver tissue in all group rats. ALR expressions were lower in normal control groups , and were higher in ALR treat groups. The results of image analysis by immunohistochemistry were (0.109 +/- 0.01), (0.159 +/- 0.02) and (0.198 +/- 0.04) in normal groups, model groups and ALR groups respectively. There were significant differences between model and normal groups ( P < 0.01) as well as ALR groups and model groups (P < 0.01). The results of Western blotting were similar to immunohistochemistry. The specific 550bp gene fragments that were amplified by RT-PCR with a couple of especial primers were found in ALR group but not in normal and model groups, which was coincident with fragments amplified by PCR with ALR recombinant plasmid extracted directly from competent E. coli DH5.


CONCLUSIONS
The expression of ALR was relatively low abundance in normal rat liver tissue, and increased markedly in immune injured rat liver tissue. The expression of the pcDNA3-ALR recombinant plasmid can be found in rat liver tissue suffered from immune hepatic fibrosis. The reinforcements of expression of ALR protein in liver tissue of pcDNA3-ALR recombinant plasmid treatment group was resulted from the overlap of endogenous and exogenous ALR expression.
Li
Qing
Q
Department of Epidemiology, Hebei Medical University, Shijiazhuang 050017, China. liqing65@tom.com
Liu
Dianwu
D
He
Haiyan
H
Xiao
Yonghong
Y
D000818:Animals; D015870:Gene Expression; D015316:Genetic Therapy; D008099:Liver / Q000378:metabolism*; D008103:Liver Cirrhosis / Q000378:metabolism* / Q000628:therapy; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D010957:Plasmids; D011506:Proteins / Q000235:genetics / Q000378:metabolism / Q000627:therapeutic use*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000627:therapeutic use*; D050862:Oxidoreductases Acting on Sulfur Group Donors
D011506:Proteins; D011994:Recombinant Proteins; C088825:GFER protein, rat; D050862:Oxidoreductases Acting on Sulfur Group Donors