Comparative analysis of serum proteomes to discover biomarkers for ossification of the posterior longitudinal ligament.
METHODS
Serum proteomes from normal subjects and the patients with ossification of the posterior longitudinal ligament (OPLL) were analyzed by using proteomics.


OBJECTIVE
To identify novel serologic biomarkers for diagnosing OPLL.


BACKGROUND
OPLL can compress the spinal cord, and special planning is required for surgeries that are done from the front of the cervical spine. However, the definitive serologic biomarkers for OPLL are still unclear.


METHODS
The 2-dimensional electrophoresis patterns of sera from OPLL patients and normal subjects were compared. The differentially expressed spots were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and electrospray ionization quadruple time-of-flight mass spectrometry.


RESULTS
Nine spots that were differentially expressed in the sera of OPLL patients were found and were identified. PRO2675, human serum albumin in a complex with myristic acid and tri-iodobenzoic acid, an unknown protein, chain B of the crystal structure of deoxy-human hemoglobin beta6, pro-apolipoprotein, ALB protein, retinol binding protein and chain A of human serum albumin mutant R218h complexed with thyroxine (3,3',5,5', tetraiodo-L-thyronine), were up-regulated in the sera of OPLL patients, whereas alpha1-microglobulin/bikunin precursor was down-regulated.


CONCLUSIONS
These proteins could be used as diagnostic biomarkers of OPLL.
Eun
Jong-Pil
JP
Department of Pharmacology, Research Institute of Clinical Medicine, Chonbuk National University Medical School, Jeonbuk, Republic of Korea.
Ma
Tian-Ze
TZ
Lee
Woo-Jong
WJ
Kim
Min-Gul
MG
Yoo
Min-Jeong
MJ
Koh
Eun-Jung
EJ
Choi
Ha-Young
HY
Kwak
Yong-Geun
YG
D000328:Adult; D001053:Apolipoproteins / Q000097:blood / Q000235:genetics; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006023:Glycoproteins / Q000097:blood / Q000235:genetics; D006454:Hemoglobins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D008875:Middle Aged; D017887:Ossification of Posterior Longitudinal Ligament / Q000097:blood* / Q000175:diagnosis*; D011498:Protein Precursors / Q000097:blood / Q000235:genetics; D020543:Proteome / Q000378:metabolism*; D012177:Retinol-Binding Proteins / Q000235:genetics / Q000378:metabolism; D012709:Serum Albumin / Q000235:genetics / Q000378:metabolism; D000075462:Serum Albumin, Human; D015854:Up-Regulation
C422455:ALB protein, human; D001053:Apolipoproteins; D015415:Biomarkers; D006023:Glycoproteins; D006454:Hemoglobins; D011498:Protein Precursors; D020543:Proteome; D012177:Retinol-Binding Proteins; D012709:Serum Albumin; C100003:deoxyhemoglobin; D000075462:Serum Albumin, Human