title: Differentiation of Bacillus anthracis, B. cereus, and B. thuringiensis by using pulsed-field gel electrophoresis.
abstract: A pulsed-field gel electrophoresis (PFGE) method was developed for discriminating Bacillus anthracis from B. cereus and B. thuringiensis. A worldwide collection of 25 B. anthracis isolates showed high-profile homology, and these isolates were unambiguously distinguished from B. cereus and B. thuringiensis isolates by cluster analysis of the whole-genome macrorestriction enzyme digestion patterns generated by NotI.
authors.affiliation: Bioscience Division, MS M888, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
authors.forename: Wenwan
authors.initials: W
authors.lastname: Zhong
authors.forename: Yulin
authors.initials: Y
authors.lastname: Shou
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Yoshida
authors.forename: Babetta L
authors.initials: BL
authors.lastname: Marrone
mesh_terms: D001408:Bacillus anthracis / Q000145:classification* / Q000235:genetics; D001409:Bacillus cereus / Q000145:classification* / Q000235:genetics; D001413:Bacillus thuringiensis / Q000145:classification* / Q000235:genetics; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000032:analysis* / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D010802:Phylogeny
chemical_list: D004269:DNA, Bacterial; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases