Effects of beta-glucan from Aureobasidium pullulans on acute inflammation in mice.
The effects of beta-glucan isolated from Aureobasidium pullulans were observed on acute xylene-induced inflammation. beta-glucan at a dose of 62.5, 125 or 250 mg/kg were administered once orally to xylene-treated mice (0.03 mL of xylene was applied on the anterior surface of the right ear to induce inflammation), and the body weight change, ear weight, histological profiles and histomorphometrical analyses of ear were conducted upon sacrifice. The xylene was topically applied 30 min after dosing with beta-glucan. The results were compared to those of diclofenac, indomethacin and dexamethasone (15 mg/kg injected once intraperitoneally). All animals were sacrificed 2 h after xylene application. Xylene application resulted in marked increases in induced ear weights compared to that of intact control ear; hence, the differences between intact and induced ear were also significantly increased. The histological characteristics of acute inflammation, such as severe vasodilation, edematous changes of skin and infiltration of inflammatory cells, were detected in xylene-treated control ears with marked increase in the thickness of the ear tissues. However, these xylene-induced acute inflammatory changes were significantly and dose-dependently decreased by beta-glucan treatment. We conclude that beta-glucan from A. pullulans has a somewhat favorable effect in the reduction of the acute inflammatory responses induced by xylene application in mice.
Kim
Hyeong-Dong
HD
Department of Physical Therapy, College of Health Science, Catholic University of Daegu 712-702, Korea.
Cho
Hyung-Rae
HR
Moon
Seung-Bae
SB
Shin
Hyun-Dong
HD
Yang
Kun-Ju
KJ
Park
Bok-Ryeon
BR
Jang
Hee-Jeong
HJ
Kim
Lin-Su
LS
Lee
Hyeung-Sik
HS
Ku
Sae-Kwang
SK
D000208:Acute Disease; D000818:Animals; D001203:Ascomycota / Q000737:chemistry*; D001835:Body Weight / Q000187:drug effects; D004431:Ear, External / Q000187:drug effects / Q000473:pathology; D007249:Inflammation / Q000188:drug therapy*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D047071:beta-Glucans / Q000627:therapeutic use*
D047071:beta-Glucans