title: Accumulation of macrophages expressing MRP8 and MRP14 in skin lesions during Leishmania major infection in BALB/c and RAG-2 knockout mice.
abstract: Migration inhibitory factor-related protein 8 (MRP8) and MRP14 are expressed by myeloid cells and especially known as marker proteins of an immature and inflammatory subtype of macrophages. In this study, we immunohistochemically examined an accumulation of MRP8+ and MRP14+ macrophages in skin lesions during Leishmania major infection in susceptible BALB/c and RAG-2-/- mice. L. major infection caused the development of a nodular type of skin lesion at the infection site in mice and a massive accumulation of macrophages was observed in the lesions at four weeks after the infection. Immunohistochemical analyses showed MRP8+ and MRP14+ macrophages are predominant cell types in the skin lesions in both mouse strains. In contrast, F4/80+ cells, which correspond to mature macrophages, were rarely found in the skin lesions. These data suggest that the accumulation of inflammatory subtype of macrophages in BALB/c mice during L. major infection can be induced without acquired immune responses.
authors.affiliation: Department of Molecular Immunology, School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo, Japan.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Goto
authors.forename: Chizu
authors.initials: C
authors.lastname: Sanjoba
authors.forename: Nana
authors.initials: N
authors.lastname: Arakaki
authors.forename: Masaaki
authors.initials: M
authors.lastname: Okamoto
authors.forename: Keiichi
authors.initials: K
authors.lastname: Saeki
authors.forename: Takashi
authors.initials: T
authors.lastname: Onodera
authors.forename: Mamoru
authors.initials: M
authors.lastname: Ito
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Matsumoto
mesh_terms: D000818:Animals; D040501:Calgranulin A / Q000378:metabolism*; D040502:Calgranulin B / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics; D018320:Leishmania major / Q000472:pathogenicity*; D016773:Leishmaniasis, Cutaneous / Q000276:immunology* / Q000469:parasitology* / Q000473:pathology; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D012867:Skin / Q000276:immunology* / Q000378:metabolism
chemical_list: D040501:Calgranulin A; D040502:Calgranulin B; D004268:DNA-Binding Proteins; C499308:Rag2 protein, mouse