[Significances of gene differential expression patterns in hepatocirrhosis and non-hepatocirrhosis tissues within different ischemic time].
OBJECTIVE
To investigate the gene differential expression patterns in hepatocirrhosis and non-hepatocirrhosis tissues within different ischemic time.


METHODS
The liver tissues were divided into two groups: Group A (non-hepatocirrhosis), Group B (hepatocirrhosis), each of which consisted of 3 groups with different ischemic time: 15, 30 and 45 minutes. The gene differential expression patterns in the two groups within different ischemic time were detected and compared with those in normal liver tissues by using 4000 points gene microarray.


RESULTS
In non-hepatocirrhosis tissues, the homeostatic maintenance genes expressed highly during hepatic ischemia for 15 minutes, and no apoptotic gene was expressed; but in hepatocirrhosis tissues, many apoptotic genes expressed highly. As for 30 minutes, in both two groups liver tissue genes expressed to the peak, and the genes related to cell death, oxidative stress and nuclear factors expressed highly. The difference lies in the facts that in Group B pro-apoptosis genes expressed more than those in Group A, and the Ratio values were higher than those in Group A. Many genes of heat shock protein family and antioxidant proteins expressed highly simultaneously in Group A, but comparatively low in Group B. As for 45 minutes, genes of heat shock proteins and antioxidant proteins expressed lowly in Group B.


CONCLUSIONS
It suggests that the safe time limit of hepatic ischemia for cell survive is 30 minutes or so. Non-hepatocirrhosis tissues could endure 30 minutes of ischemia and even longer, but it should be restricted within 30 minutes in hepatocirrhosis tissues.
Lu
Qi-ping
QP
Department of General Surgery, Wuhan General Hospital of Guangzhou Command, Wuhan 430070, China. ptwklqp@yahoo.com.cn
Qiu
Fa-zu
FZ
Wu
Zai-de
ZD
Cao
Ting-jia
TJ
Zhang
Zhi-yong
ZY
Cao
Jun
J
Liu
Wei
W
Feng
Yu-ling
YL
D020869:Gene Expression Profiling; D006801:Humans; D007511:Ischemia / Q000235:genetics*; D008099:Liver / Q000098:blood supply* / Q000378:metabolism; D008103:Liver Cirrhosis / Q000235:genetics* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D013997:Time Factors