Endogenous sphingolipid metabolites related to the growth in Sphingomonas chungbukensis.
Sphingolipids are present in animals, plants, fungi, yeasts and some bacteria. In mammalian cells sphingolipids act as lipid mediators for cell growth, differentiation, apoptosis and angiogenesis. In contrast, in bacteria the biological significance of sphingolipids has not been fully elucidated and sphingolipid metabolism has not been investigated. The aim of this study was to compare the pattern of sphingolipid metabolites in HIT-T15 beta cells originating from hamster pancreas to that in the bacterial strain Sphingomonas chungbukensis DJ77, under various culture conditions. It was found that the concentration of cellular sphinganine (Sa) in S. chungbukensis was higher than that of sphingosine (So), while the level of cellular So in HIT-T15 cells was higher than that of Sa. Aeration and shaking during culture increased bacterial growth in S. chungbukensis, and the contents of So and Sa were also elevated. These results indicate that a de novo sphingolipid pathway appeared to be active in bacteria and that bacterial growth may be closely related to Sa levels.
Burenjargal
Munkhtsatsral
M
College of Pharmacy and CBITRC, Chungbuk National University, Cheongju 361-763, Korea.
Lee
Youn-Sun
YS
Yoo
Jae-Myung
JM
Kim
Young-Chang
YC
Lee
Yong-Moon
YM
Oh
Seikwan
S
Yun
Yeo-Pyo
YP
Hong
Jin-Tae
JT
Chung
Youn-Bok
YB
Moon
Dong-Chul
DC
Yoo
Hwan-Soo
HS
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D050417:Insulin-Secreting Cells / Q000378:metabolism; D013107:Sphingolipids / Q000096:biosynthesis*; D020578:Sphingomonas / Q000254:growth & development* / Q000378:metabolism; D013997:Time Factors
D013107:Sphingolipids