Cross-species chromosome painting.
Comparative genomics is an important and expanding field of research, and the genome-wide comparison of the chromosome constitution of different species makes a major contribution to this field. Cross-species chromosome painting is a powerful technique for establishing chromosome homology maps, defining the sites of chromosome fusions and fissions, investigating chromosome rearrangements during evolution and constructing ancestral karyotypes. Here the protocol for cross-species chromosome painting is presented. It includes sections on cell culture and metaphase preparation, labeling of chromosome-specific DNA, fluorescent in situ hybridization (chromosome painting) and image analysis. Cell culture and metaphase preparation can take between 1 and 2 wk depending on the cell culture. Labeling of chromosome-specific DNA is completed in 1 d. Fluorescent in situ hybridization can be completed in a maximum of 4 d.
Rens
Willem
W
Centre for Veterinary Science, Department of Veterinary Medicine, University of Cambridge, Cambridge CB3 OES, UK. wr203@mole.bio.cam.ac.uk
Fu
Beiyuan
B
O'Brien
Patricia C M
PC
Ferguson-Smith
Malcolm
M
D000818:Animals; D002478:Cells, Cultured; D020223:Chromosome Painting / Q000379:methods*; D004247:DNA / Q000032:analysis*; D023281:Genomics / Q000379:methods; D013045:Species Specificity
D004247:DNA