title: [Effect of platelet CD42a modification by mPEG-SPA with different molecular masses].
abstract: OBJECTIVE
  To observe the effect platelet antigen modification by mPEG-SPA with different molecular masses.
  
  
  METHODS
  Platelet CD42a was modified by 5 kD and 20 kD mPEG-SPA, respectively, and the fluorescence intensity of CD42a was detect by flow cytometry and the three-dimensional structure of CD42a simulated to analyze the distribution of lysine in CD42a molecule.
  
  
  RESULTS
  After platelet CD42a modification by 5 kD and 20 kD mPEG-SPA, the fluorescence intensity of CD42a decreased sharply by 85.54% and 88.65%, respectively, and multiple lysine regions were identified on the surface of CD42a molecule.
  
  
  CONCLUSIONS
  Both 5 kD and 20 kD mPEG-SPA allow useful modification of platelet CD42a, but 20 kD mPEG-SPA is more advantageous than 5 kD mPEG-SPA.
authors.affiliation: Shenzhen Institute of Transfusion Medicine, Shenzhen 518035, China. zyz200157@sohu.com
authors.forename: Yin-ze
authors.initials: YZ
authors.lastname: Zhang
authors.forename: Wen
authors.initials: W
authors.lastname: Xiong
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
authors.forename: Chao-peng
authors.initials: CP
authors.lastname: Shao
authors.forename: Tian-jun
authors.initials: TJ
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Zhao
authors.forename: Bao-cheng
authors.initials: BC
authors.lastname: Yang
mesh_terms: D001792:Blood Platelets / Q000737:chemistry*; D006801:Humans; D008970:Molecular Weight; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D013388:Succinimides / Q000737:chemistry*
chemical_list: D019038:Platelet Glycoprotein GPIb-IX Complex; D013388:Succinimides; D011092:Polyethylene Glycols; C028210:monomethoxypolyethylene glycol