Human herpesvirus 7 infection increases the expression levels of CD46 and CD59 in target cells.
CD46 (membrane cofactor protein; MCP) is a molecule that functions as either a complement-regulatory protein (CRP) or a receptor for some pathogens, including human herpesvirus 6. DNA microarray analysis suggested that the expression of CD46 was upregulated in T cells infected with human herpesvirus 7 (HHV-7). Northen and Western blot analyses supported this result at both the transcriptional and translational levels. Flow-cytometric analysis revealed that upregulation of CD46 occurred at a late stage of infection in both SupT1 cells and primary CD4+ T cells, and also that expression of another CRP, CD59, was increased at a late stage of infection. Whether these CRPs actually function in HHV-7-infected cells was addressed and it was found that HHV-7-infected cells were more resistant to complement-dependent cytotoxicity than were uninfected cells. This study is the first report demonstrating that HHV-7 infection causes elevation of the CRPs CD46 and CD59, which may be a possible mechanism for HHV-7 to evade humoral immunity via complement.
Takemoto
Masaya
M
Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation, 7-6-8 Saito-Asagi, Ibaraki, Osaka 567-0085, Japan.
Yamanishi
Koichi
K
Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation, 7-6-8 Saito-Asagi, Ibaraki, Osaka 567-0085, Japan.
Mori
Yasuko
Y
Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation, 7-6-8 Saito-Asagi, Ibaraki, Osaka 567-0085, Japan.
D000917:Antibody Formation; D015703:Antigens, CD / Q000235:genetics; D015152:Blotting, Northern; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000821:virology; D018957:CD59 Antigens / Q000235:genetics*; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology / Q000821:virology; D005786:Gene Expression Regulation / Q000276:immunology*; D016199:Herpesvirus 7, Human; D006801:Humans; D051916:Membrane Cofactor Protein / Q000235:genetics*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D019349:Roseolovirus Infections / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D015703:Antigens, CD; D018957:CD59 Antigens; D051916:Membrane Cofactor Protein; D012313:RNA