[Tracking Trojan peptides in cells].
Trojan peptides or cell-penetrating peptides (CPP) are natural or designed peptides identified as cellular membrane-crossing molecules, in particular through their potency to vehiculate various kinds of compounds to the cytoplasm and nucleus of living cells. The indirect methods used so far to detect these peptides in cells led to controversial hypotheses on the mechanism of their cell entry. Therefore, we have developed a MALDI-TOF mass spectrometry-based quantification method to track these peptides inside cells. This new method is presented in this review.
Sagan
Sandrine
S
Université Pierre et Marie Curie, UMR 7613 CNRS, case courrier 182, 4 place Jussieu, 75252 Paris cedex 05, France. sagan@ccr.jussieu.fr
Burlina
Fabienne
F
Delaroche
Diane
D
Aussedat
Baptiste
B
Aubry
Soline
S
Bolbach
Gérard
G
Lavielle
Solange
S
Chassaing
Gérard
G
D021581:Active Transport, Cell Nucleus; D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells / Q000737:chemistry; D002352:Carrier Proteins / Q000032:analysis; D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability; D002467:Cell Nucleus / Q000378:metabolism; D057846:Cell-Penetrating Peptides; D006224:Cricetinae; D003412:Cricetulus; D003593:Cytoplasm / Q000378:metabolism; D015696:Gene Products, tat / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology; D010447:Peptide Hydrolases / Q000378:metabolism; D010455:Peptides / Q000032:analysis / Q000378:metabolism*; D021381:Protein Transport; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D054322:tat Gene Products, Human Immunodeficiency Virus
D002352:Carrier Proteins; D057846:Cell-Penetrating Peptides; D015696:Gene Products, tat; D010446:Peptide Fragments; D010455:Peptides; D054322:tat Gene Products, Human Immunodeficiency Virus; C422951:tat peptide (48-60), Human immunodeficiency virus 1; C414409:penetratin; D010447:Peptide Hydrolases