Affinity precipitation an option for early capture in bioprocessing.
Affinity precipitation is a bioseparation technique where the affinity ligand is coupled to a stimuliresponsive polymer. Stimuli-responsive polymers show abrupt, yet reversible, phase transition (precipitation) in response to a small change in an environmental parameter. The corresponding ligand conjugates can be used to co-precipitate and thereby capture and isolate target molecules from complex solutions such as culture supernatants and cell lysates. The approach is compatible with a 'discardibles only' type of downstream process and can be scaled over several orders of magnitude. This report discusses the set-up and development of affinity precipitation procedures, the related instrumentation and scale up, as well as applications for the isolation of proteins and polynucleotides.
Freitag
Ruth
R
University of Bayreuth, Bayreuth, Germany. bioprozesstechnik@uni-bayreuth.de
Schumacher
Ivo
I
Hilbrig
Frank
F
D005591:Chemical Fractionation / Q000379:methods*; D002846:Chromatography, Affinity / Q000379:methods*; D047468:Immunoprecipitation / Q000379:methods*; D009711:Nucleotides / Q000737:chemistry* / Q000302:isolation & purification*; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification*
D009711:Nucleotides; D011506:Proteins