Use of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide for rapid detection of methicillin-resistant Staphylococcus aureus by resonance light scattering.
A rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) using resonance light scattering (RLS) on an ordinary fluorescence spectrometer was developed. The viable MRSA reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) to produce insoluble particles which displayed intense resonance scattering light. It showed a linear relationship between the number of viable MRSA and the RLS intensity. Dead MRSA were unable to reduce MTT. MRSA exposed to flavonoids extracted from Marchantia convoluta (MCF) showed a MCF concentration-dependent inhibition of the ability to reduce MTT. The RLS could, in combination with the MTT assay, be a rapid and sensitive detection method for vitro-cultured MRSA.
Xiao
Jian Bo
JB
College of Chemistry and Chemical Engineering, Central South University, Changsha, PR China.
Chen
Jing Wen
JW
Ren
Feng Lian
FL
Yang
Chun Sheng
CS
Xu
Ming
M
D015169:Colony Count, Microbial; D005419:Flavonoids / Q000302:isolation & purification; D008027:Light; D016106:Methicillin Resistance; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013211:Staphylococcus aureus / Q000302:isolation & purification*; D013778:Tetrazolium Salts / Q000737:chemistry*; D013844:Thiazoles / Q000737:chemistry*
D005419:Flavonoids; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue