[Tryptose sulphite cycloserine agar for the recovery of Clostridium perfringens in surface waters: a study of different modes of utilization].
In the recent European Drinking Water Directive, Clostridium perfringens has assumed increasing importance so as to be considered a primary contamination indicator. Therefore it emerged the necessity to make culture methods, aimed at its recovery, more specific and sensitive. In this study we have verified the ability of Tryptose Sulphite Cycloserine Agar plates (TSC Agar), prepared and stored before the use at refrigeration temperature (+4 degrees) for different times, to show typical colonies, using both, the single layer and double layer techniques. Results show that storage of the prepared medium, even for a few days, decrease the recovery of typical colonies although such negative effect is minimized by using the double layer technique.
Nusca
A
A
Dipartimento Ambiente e Connessa Prevenzione Primaria, Istituto Superiore di Sanità, Roma. assunta.nusca@iss.it
Orefice
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L
Paradiso
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R
D000362:Agar; D003016:Clostridium perfringens / Q000302:isolation & purification*; D015169:Colony Count, Microbial / Q000379:methods*; D003470:Culture Media / Q000737:chemistry*; D003523:Cycloserine; D006801:Humans; D009930:Organic Chemicals; D012189:Retrospective Studies; D045483:Rivers; D013447:Sulfites; D014871:Water Microbiology; D014881:Water Supply / Q000592:standards*
D003470:Culture Media; D009930:Organic Chemicals; D013447:Sulfites; C046666:tryptose; D000362:Agar; D003523:Cycloserine