title: Acute cerebral vascular injury after subarachnoid hemorrhage and its prevention by administration of a nitric oxide donor.
abstract: OBJECTIVE
  Structural changes in brain parenchymal vessels occur within minutes after subarachnoid hemorrhage (SAH). These changes include platelet aggregation, activation of vascular collagenases, and destruction of perivascular collagen IV. Because collagen IV is an important component of the basal lamina, the authors attempted to further define changes in vascular structure (length and luminal diameter) and their relationship to vascular permeability immediately after SAH. In addition, the authors explored whether such alterations were attenuated by administration of a nitric oxide (NO) donor.
  
  
  METHODS
  Endovascular perforation was used to induce SAH in rats. Two sets of experiments were performed. The first established changes in vascular structure and permeability (collagen IV and endothelial barrier antigen [EBA] dual immunofluorescence) during the first 24 hours after SAH. In the second, the investigators examined the effects of an NO donor on vascular structure, permeability, and collagenase activity (in situ zymography). In this second study, animals received intravenous infusion of the NO donor S-nitrosoglutathione (GSNO, 1 microM/8 microl/min) 15 minutes after induction of SAH and were killed 3 hours after SAH onset. Controls were naive unoperated animals for the first study and saline-infused SAH animals for the second. The authors found a time-dependent decrease in area fraction, length, and luminal diameter of collagen IV- and EBA-immunofluorescent vessels after SAH. The greatest change occurred at 3 hours after onset of SAH. Administration of GSNO was associated with striking preservation of collagen IV and EBA immunofluorescence compared with saline treatment. Zymography indicated decreased collagenase activity in GSNO-treated SAH animals compared with saline-treated SAH animals.
  
  
  CONCLUSIONS
  These results demonstrate changes in the structure and permeability of brain parenchymal microvessels after SAH and their reversal by treatment with an NO donor.
authors.affiliation: Department of Neurosurgery, Mount Sinai School of Medicine, New York, New York 10029-6574, USA. fatima.sehba@mssm.edu
authors.forename: Fatima A
authors.initials: FA
authors.lastname: Sehba
authors.forename: Victor
authors.initials: V
authors.lastname: Friedrich
authors.forename: Girma
authors.initials: G
authors.lastname: Makonnen
authors.forename: Joshua B
authors.initials: JB
authors.lastname: Bederson
mesh_terms: D000818:Animals; D001921:Brain / Q000098:blood supply* / Q000473:pathology / Q000503:physiopathology; D002199:Capillary Permeability / Q000502:physiology; D008297:Male; D008833:Microcirculation / Q000473:pathology / Q000503:physiopathology; D020030:Nitric Oxide Donors / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D026422:S-Nitrosoglutathione / Q000627:therapeutic use*; D013345:Subarachnoid Hemorrhage / Q000150:complications* / Q000473:pathology / Q000517:prevention & control*; D013997:Time Factors
chemical_list: D020030:Nitric Oxide Donors; D026422:S-Nitrosoglutathione