[Identification of novel genes related to glioma by oligonucleotide microarray].
OBJECTIVE
To identify the genes differentially expressed in development of human glioma, and to study the expression of some genes in different grade gliomas.


METHODS
Oligonucleotide microarray (including 218 genes related to neural system development) was adopted and hybridized with probes which were prepared from the total RNAs of glioma specimens and normal brain tissues. Differentially expressed genes between the normal tissues and glioma tissues were assayed after scanning oligonuceltide microarray with ScanArray 4000, and some of these genes such as smad1, Hmp19 and TRIP3 were verified by real-time quantitative PCR(real-time-Q-PCR) method.


RESULTS
In comparison with the genes in the normal brain tissue, 5 down-regulated and 5 up-regulated genes in glioma specimens were revealed by means of microarrays, and the expression of smad1, Hmp19 and TRIP3 were verified by real-time-Q-PCR assay.


CONCLUSIONS
Multiple genes play important roles in development of glioma. cDNA microarray technology is a powerful technique in screening for differentially expressed genes between glioma tissues and normal brain tissues. This study is helpful for judgement of invasion and prognosis of gliomas, and provides more target genes for targeted therapy.
Liu
Shuang
S
Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing, 100850 PR China.
Tian
Zeng-min
ZM
Zheng
Guo-qiang
GQ
Wu
Hai-tao
HT
Jin
Yan-bin
YB
Ma
Xin
X
Fan
Wen-hong
WH
Fan
Ming
M
D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction