title: Immunocytochemical technique for protein localization in sections of plant tissues.
abstract: There is a growing demand for methods that allow rapid and reliable in situ localization of proteins in plant cells. The immunocytochemistry protocol presented here can be used routinely to observe protein localization patterns in tissue sections of various plant species. This protocol is especially suitable for plant species with more-complex tissue architecture (such as maize, Zea mays), which makes it difficult to use an easier whole-mount procedure for protein localization. To facilitate the antibody-antigen reaction, it is necessary to include a wax-embedding and tissue-sectioning step. The protocol consists of the following procedures: chemical fixation of tissue, dehydration, wax embedding, sectioning, dewaxing, rehydration, blocking and antibody incubation. The detailed protocol, recommended controls and troubleshooting are presented here, along with examples of applications.
authors.affiliation: Center for Plant Molecular Biology, Tübingen University, 72076 Tübingen, Germany.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Paciorek
authors.forename: Michael
authors.initials: M
authors.lastname: Sauer
authors.forename: Jozef
authors.initials: J
authors.lastname: Balla
authors.forename: Justyna
authors.initials: J
authors.lastname: Wiśniewska
authors.forename: Jirí
authors.initials: J
authors.lastname: Friml
mesh_terms: D007150:Immunohistochemistry / Q000379:methods*; D008867:Microtomy / Q000379:methods; D010940:Plant Proteins / Q000032:analysis*; D010944:Plants / Q000378:metabolism
chemical_list: D010940:Plant Proteins