title: Glycoconjugate nanoribbons from the self-assembly of carbohydrate-peptide block molecules for controllable bacterial cell cluster formation.
abstract: We demonstrate here the rational design strategy to control the length of 1-dimensional beta-sheet peptide nanoassembly. We synthesized the beta-sheet peptides with attached coils and carbohydrates. We reasoned that the bulkiness of the coils affects the final length of the assembled beta-sheet peptide nanostructures because of the steric crowding effect. The nanostructure from the peptide with a small and linear coil was several micrometers long, whereas the one from the peptide with a high-volume-fraction dendritic coil was only about 150 nm long. For potential biological applications of the peptide nanoassemblies, we investigated the interactions of the carbohydrate-coated nanoassemblies with E. coli cells containing cognate binding proteins. The results showed that both of the nanoassemblies could immobilize and/or aggregate bacterial cells. The degrees of immobilization were similar for both nanoassemblies; however, only the long nanoribbon was shown to induce the formation of bacterial clusters.
authors.affiliation: Center for Supramolecular Nano-Assembly and Department of Chemistry, and Department of Biochemistry, Yonsei University, Seoul 120-749, Korea.
authors.forename: Yong-Beom
authors.initials: YB
authors.lastname: Lim
authors.forename: Somi
authors.initials: S
authors.lastname: Park
authors.forename: Eunji
authors.initials: E
authors.lastname: Lee
authors.forename: Haemi
authors.initials: H
authors.lastname: Jeong
authors.forename: Ja-Hyoung
authors.initials: JH
authors.lastname: Ryu
authors.forename: Myeong Sup
authors.initials: MS
authors.lastname: Lee
authors.forename: Myongsoo
authors.initials: M
authors.lastname: Lee
mesh_terms: D001419:Bacteria / Q000737:chemistry*; D002241:Carbohydrates / Q000737:chemistry; D018914:Cells, Immobilized / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry; D006020:Glycopeptides / Q000138:chemical synthesis / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D017433:Protein Structure, Secondary
chemical_list: D002241:Carbohydrates; D006020:Glycopeptides