Determination of eprosartan in human plasma and urine by LC/MS/MS.
A protein precipitation, liquid chromatography/tandem mass spectrometry (LC/MS/MS) method has been developed and validated for the determination of eprosartan in human plasma and urine. The solvent system also served as a protein precipitation reagent. The chromatographic separation was achieved on a CAPCELL PAK C18 column (50 mmx2.0 mm, 5 microm, Shiseido). A mobile phase was consisted of 0.5% formic acid in water and 0.5% formic acid in acetonitrile (72:28). Detection was by positive ion electrospray tandem mass spectrometry on a Sciex API3000. The standard curves, which ranged from 5 to 2000 ng/mL in human plasma and from 0.25 to 50 microg/mL in urine, were fitted to a 1/x weighted quadratic regression model. The method proved to be accurate, specific and sensitive enough to be successfully applied to a pharmacokinetic study.
Li
Xue-Ning
XN
Department of Clinical Pharmacology, ZhongShan Hospital, Fudan University, 180 FengLin Road, Shanghai 200032, China. xnlee@zshospital.com
Xu
Hong-Rong
HR
Chen
Wei-Li
WL
Liu
Gang-Yi
GY
Chu
Nan-Nan
NN
Yu
Chen
C
D000179:Acrylates / Q000097:blood* / Q000737:chemistry / Q000652:urine*; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D007093:Imidazoles / Q000097:blood* / Q000737:chemistry / Q000652:urine*; D015394:Molecular Structure; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013876:Thiophenes / Q000097:blood* / Q000737:chemistry / Q000652:urine*
D000179:Acrylates; D007093:Imidazoles; D013876:Thiophenes; C068373:eprosartan