title: [Detection and typification of human papilloma virus in pre cancerous cervical lesions].
abstract: BACKGROUND
  The association of different genotypes of human papilloma virus (HPV) with cervical cancer is well known. However, there is little information about their association with pre-cancerous lesions.
  
  
  OBJECTIVE
  To assess the frequency of different HPV genotypes in pre cancerous cervical lesions.
  
  
  METHODS
  A cervical sample was obtained by cytobrush in 15 women with low grade lesions and 40 women with high grade lesions, subjected to conization by loop electrical excision procedure (LEEP). Detection and typification of HPV was done by polymerase chain reaction and restriction fragment length polymorphism.
  
  
  RESULTS
  All women were infected with HPV. Eighty five percent of samples were typified. A unique HPV subtype was found in 76% of women. Fourteen percent had an infection with multiple subtypes and in 10%, the viral genotype was not identified. The most common subtypes found were HPV 16, HPV 52 and HPV 53.
  
  
  CONCLUSIONS
  There is a high rate of infection with HPV with a high oncogenic risk among these women.
authors.affiliation: Departamento de Anatomía Patológica, Facultad de Medicina, Universidad de La Frontera, Temuco, Chile.
authors.forename: Susana
authors.initials: S
authors.lastname: Aedo A
authors.forename: Angélica
authors.initials: A
authors.lastname: Melo A
authors.forename: Patricia
authors.initials: P
authors.lastname: García
authors.forename: Pablo
authors.initials: P
authors.lastname: Guzmán G
authors.forename: Italo
authors.initials: I
authors.lastname: Capurro V
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Roa S
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D054458:Amplified Fragment Length Polymorphism Analysis; D015344:DNA Probes, HPV / Q000235:genetics / Q000302:isolation & purification; D004279:DNA, Viral / Q000302:isolation & purification*; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000150:complications / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D011230:Precancerous Conditions / Q000821:virology*; D012720:Severity of Illness Index; D002578:Uterine Cervical Dysplasia / Q000821:virology*; D002583:Uterine Cervical Neoplasms / Q000821:virology*; D014626:Vaginal Smears
chemical_list: D015344:DNA Probes, HPV; D004279:DNA, Viral