[Mimic epitope of aflatoxin B1 screened by phage display technique].
OBJECTIVE
To screen mimic epitope from phage-display random peptide libraries as sources of peptides that mimic the binding of aflatoxin B1 to monoclonal antibodies raised against the toxin and establish immunoassay for aflatoxin B1.


METHODS
The monoclonal antibody against the aflatoxin B1 was used as ligand to screen the binding peptide from the Ph.D.-7 peptide library with phage display and the specificity of colons were identified by ELISA. The peptide sequences of positive phage clones were determined and analyzed by DNA sequencing.


RESULTS
After four rounds of panning, the binding peptides were screened from random peptide libraries. Through indirect competitive ELISA, 4 positive clones could inhibited aflatoxin B1 and HXXDPXH was characterized by DNA sequencing, X is random amino acid. Then competitive ELISA was established for clone phage 10, the linear ranges of the inhibition curves were between 100 pg/ml and 2000 pg/ml, the detecting limit was 50 pg/ml.


CONCLUSIONS
The mimic epitope of aflatoxin B1 antigen was obtained successfully by the phage display technigue and it is effective substitutes for aflatoxin B1-protein conjugate in the immunoassay.
Deng
Shengliang
S
Sino-German Joint Reserch Institute, the Key Laboratory of Food Science of MOE, Nanchang University, Nanchang 330047, China.
Xu
Yang
Y
Liu
Renrong
R
D016604:Aflatoxin B1 / Q000235:genetics / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000939:Epitopes / Q000276:immunology*; D018716:Molecular Mimicry; D019151:Peptide Library
D000911:Antibodies, Monoclonal; D000939:Epitopes; D019151:Peptide Library; D016604:Aflatoxin B1