Interaction of acetylcholinesterase inhibitor donepezil with ionic channels of the neuronal membrane.
The effects of donepezil on voltage-dependent Ca(2+)- and low-threshold K(+)-current were studied on isolated molluscan neurons using two-electrode voltage clamp technique. Donepezil reduced the amplitude of voltage-dependent Ca(2+)-current (IC(50)=7.9 microM) and shifted the current-voltage relationships toward hyperpolarization. Donepezil in low concentration (5 microM) increased, while in higher concentrations (>or=10 microM) decreased the low-threshold K(+)-current.
Solntseva
E I
EI
Institute of Brain Research, Russian Academy of Medical Sciences, Moscow.
Bukanova
Yu V
YV
Skrebitskii
V G
VG
D000818:Animals; D015220:Calcium Channels / Q000187:drug effects / Q000502:physiology; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D000077265:Donepezil; D006372:Helix, Snails; D007189:Indans / Q000494:pharmacology*; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D008839:Microelectrodes; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D018408:Patch-Clamp Techniques; D010880:Piperidines / Q000494:pharmacology*
D015220:Calcium Channels; D002800:Cholinesterase Inhibitors; D007189:Indans; D007473:Ion Channels; D010880:Piperidines; D000077265:Donepezil