Clostridium perfringens type A toxin production in 3 commonly used culture media.
In vitro toxin production is an important tool not only for diagnostic purposes but also for the study of pathogenesis of Clostridium perfringens infections. The present study was carried out to compare the level of toxin production by several strains of C. perfringens type A, isolated from the intestine of animals, when cultured in 3 different conventional culture media. Six strains of C. perfringens type A isolated from the small intestine of healthy sheep were cultured in commercial cooked meat medium (CMM), brain heart infusion (BHI), and tryptone glucose yeast (TGY). Intravenous lethality in mice and phospholipase C (PLC) activity were measured in filtered culture supernatants. Lethality of culture supernatants was highest for all isolates when grown in BHI, followed by CMM. No supernatants from any isolates grown in TGY produced lethality in mice. Phospholipase C activity was highest when the isolates were grown in BHI and CMM and significantly lower when grown in TGY.
Fernandez-Miyakawa
Mariano E
ME
California Animal Health and Food Safety Laboratory, San Bernardino Branch, Faculty of Veterinary Medicine, University of California Davis, San Bernardino, CA 92408, USA.
Marcellino
Romanella
R
Uzal
Francisco A
FA
D000818:Animals; D001427:Bacterial Toxins / Q000032:analysis* / Q000633:toxicity; D002135:Calcium-Binding Proteins / Q000032:analysis* / Q000633:toxicity; D003470:Culture Media / Q000032:analysis*; D007928:Lethal Dose 50; D051379:Mice; D012756:Sheep; D010738:Type C Phospholipases / Q000032:analysis* / Q000633:toxicity
D001427:Bacterial Toxins; D002135:Calcium-Binding Proteins; D003470:Culture Media; D010738:Type C Phospholipases; C040649:alpha toxin, Clostridium perfringens