A role for Tbx2 in the regulation of the alpha2(1) collagen gene in human fibroblasts.
The T-box gene family encodes highly conserved transcription factors that play important roles in embryonic development and have been implicated in carcinogenesis. One member of the family, Tbx2, is generally regarded as a transcriptional repressor but appears to be capable of functioning as an activator depending on the cellular context. This study shows that Tbx2 is expressed in normal human fibroblasts but is drastically reduced in several transformed fibroblast cell lines. This pattern of Tbx2 expression correlated with that observed for the human alpha2(1) collagen gene (COL1A2). Interestingly, stable expression of transfected Tbx2 in transformed fibroblast cell lines further reduces expression of the human endogenous COL1A2 gene. This ability of Tbx2 to repress the human COL1A2 gene was confirmed in luciferase reporter assays and shown to be independent of the consensus T-box binding element.
Teng
Huajian
H
Division of Cell Biology, Faculty of Health Sciences, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa.
Davis
Emily
E
Abrahams
Amaal
A
Mowla
Shaheen
S
Parker
M Iqbal
MI
Prince
Sharon
S
D002460:Cell Line; D045744:Cell Line, Tumor; D003094:Collagen / Q000378:metabolism*; D024042:Collagen Type I; D005347:Fibroblasts / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D008853:Microscopy; D009369:Neoplasms / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020825:T-Box Domain Proteins / Q000235:genetics* / Q000502:physiology*; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection
D024042:Collagen Type I; D012333:RNA, Messenger; C097348:T-Box Domain Protein 2; D020825:T-Box Domain Proteins; D014157:Transcription Factors; D003094:Collagen