title: [Cloning and characterization of down-regulated genes in neural tube defects of golden hamster induced by hyperthermia].
abstract: OBJECTIVE
  To clone down-regulated genes in neural tube defects of golden hamster induced by hyperthermia and to explore the molecular mechanism.
  
  
  METHODS
  A reversed subtractive cDNA library was constructed using suppression subtractive hybridization. Clones with inserts were selected by combination of alpha complementary phenomenon and colony PCR. Then sequence and homology analysis of the inserts were made. And mRNA expression conditions were confirmed by Northern blot.
  
  
  RESULTS
  The reversed subtractive cDNA library was successfully constructed. Fifteen recombinant clones were sequenced and recognized homologous to known genes. The results of Northern blot showed that all these genes were down-regulated in defected neural tubes induced by hyperthermia compared to normal developed neural tubes.
  
  
  CONCLUSIONS
  Some important genes are identified which might be involved in neural tube defects induced by hyperthermia.
authors.affiliation: Histology and Embryology Institution, Medical School of Shandong University, Jinan, Shandong, 250012 PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Yang
authors.forename: Ying-mao
authors.initials: YM
authors.lastname: Gao
authors.forename: Shao-ling
authors.initials: SL
authors.lastname: Li
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D003001:Cloning, Molecular; D006224:Cricetinae; D005260:Female; D015723:Gene Library; D006979:Hyperthermia, Induced / Q000009:adverse effects*; D008647:Mesocricetus; D009436:Neural Tube Defects / Q000209:etiology / Q000235:genetics*