Quick and easy yeast transformation using the LiAc/SS carrier DNA/PEG method.
Here, we describe a quick and easy version of the lithium acetate/single-stranded carrier DNA/PEG method of transformation for Saccharomyces cerevisiae. This method can be performed when only a few transformants are needed. The procedure can take less than an hour, depending on the duration of the heat shock. It can be used to transform yeast cells from various stages of growth and storage. Cells can be transformed from freshly grown cells as well as cells stored on a plate at room temperature or in a refrigerator.
Gietz
R Daniel
RD
Department of Biochemistry and Medical Genetics, University of Manitoba, T250-770 Bannatyne Ave., Winnipeg, Manitoba R3E 0W3, Canada.
Schiestl
Robert H
RH
D000085:Acetates; D004277:DNA, Single-Stranded / Q000235:genetics; D005821:Genetic Techniques; D006358:Hot Temperature; D011092:Polyethylene Glycols; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics*; D014170:Transformation, Genetic
D000085:Acetates; D004277:DNA, Single-Stranded; D011092:Polyethylene Glycols; C488804:lithium acetate