Transforming growth factor beta-1 enhances cytotoxic effect of doxorubicin in human lung adenocarcinoma cells of A549 line.
Transforming growth factor beta-1 (TGFbeta-1) is a regulator of cell proliferation, differentiation and apoptosis. Doxorubicin (adriamycin), an anthracycline drug causing double-strand DNA breaks, is widely used in anticancer chemotherapy. Here we demonstrated that TGFbeta-1 enhanced cytotoxic (proapoptotic) action of doxorubicin towards cultured human lung carcinoma A549 cells. Western-blot analysis and immunocytochemistry were used to show that doxorubicin induced PARP degradation in A549 cells, and TGFbeta-1 enhanced that action of the drug. The obtained results suggest a possibility of biomodulating effect of TGFbeta-1 on tumor cell treatment with doxorubicin.
Filyak
Yevhen
Y
Department of Regulation of Cell Proliferation, Institute of Cell Biology NAS of Ukraine, Drahomanova Street 14-16, 79005 Lviv, Ukraine.
Filyak
Oksana
O
Stoika
Rostyslav
R
D000230:Adenocarcinoma / Q000178:diet therapy; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000627:therapeutic use*; D004357:Drug Synergism; D006801:Humans; D007150:Immunohistochemistry; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000502:physiology*
D053773:Transforming Growth Factor beta1; D004317:Doxorubicin; D011065:Poly(ADP-ribose) Polymerases