title: Heat shock preconditioning induces protein carbonylation and alters antioxidant protection in superficially injured guinea pig gastric mucosa in vitro.
abstract: According to our previous studies, heat shock preconditioning of gastric mucosa requires modulation of protein synthesis and eicosanoid pathways to induce protection against superficial injury. This may be caused by heat shock-induced oxidative stress. We studied the effect of heat shock preconditioning with normothermic recovery on redox status in superficially injured (1.25 mmol NaCl for 5 min) Ussing chamber perfused guinea pig gastric mucosa allowed to recover for 3 hr after injury. Protein oxidation, lipid peroxidation, level of superoxide dismutase, level of heat shock protein 72 (HSP72), and level of oxygen radical absorbance capacity were measured. Superficial injury increased lipid peroxidation. Heat shock preconditioning decreased oxygen radical absorbance capacity and increased protein carbonyl and HSP72 levels, but inhibited electrophysiologic recovery. Exposure to indomethacin and arachidonic acid (AA) partially abolished this pro-oxidative and inhibitory effect on recovery, but maintained HSP72 levels and decreased protein carbonyls, lipid peroxidation, and oxygen radical absorbance capacity. In conclusion, superficial injury increased lipid peroxidation. Heat shock preconditioning alone induced oxidative stress via indomethacin- and AA-sensitive mechanisms. The development of optimal cytoprotective strategy may therefore require control of oxidative stress and modulation of the eicosanoid pathways.
authors.affiliation: Department of Surgery, Kuopio University Hospital, Kuopio, Finland. niku.oksala@tuubi1.net
authors.forename: Niku K J
authors.initials: NK
authors.lastname: Oksala
authors.forename: Hannu
authors.initials: H
authors.lastname: Paimela
authors.forename: Esko
authors.initials: E
authors.lastname: Alhava
authors.forename: Mustafa
authors.initials: M
authors.lastname: Atalay
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology; D019610:Cytoprotection / Q000502:physiology*; D005753:Gastric Mucosa / Q000473:pathology / Q000502:physiology*; D006168:Guinea Pigs; D050884:HSP72 Heat-Shock Proteins / Q000032:analysis; D006358:Hot Temperature; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D015227:Lipid Peroxidation; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D050050:Protein Carbonylation / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000032:analysis; D013482:Superoxide Dismutase / Q000032:analysis
chemical_list: D050884:HSP72 Heat-Shock Proteins; D017382:Reactive Oxygen Species; D016718:Arachidonic Acid; D013482:Superoxide Dismutase; D007213:Indomethacin