title: Tag STS in the AZF region associated with azoospermia in a Tunisian population.
abstract: The aim of this study was to determine the appropriate tag sequence tagged site (STS) associated with azoospermia. We evaluated the incidence of Y chromosome microdeletions in Tunisian infertile male patients by polymerase chain reaction using 14 STSs in the azoospermia factor (AZF) region of Yq11. A logistic regression analysis was performed to test the association of STSs with semen quality. Haploview version 3.11 was used to identify the possible blocks of deletion involving a minimum number of STSs and that can be used to tag the deletion block in future analysis. Using the 14 STSs, 48% infertile patients (102 of 210) had microdeletions of Y chromosome but, following the European Academy of Andrology guidelines, only 16% of patients had microdeletions. A statistically significant difference was found with some STSs for azoospermia and oligozoospermia. A candidate combinaison composed of 4 STSs (RBMY-sy157-sy84-sy130) was associated with azoospermia in a Tunisian population. According to this study, this tag can be used in the screening of Y chromosome microdeletions before assisted reproduction treatment in a Tunisian population.
authors.affiliation: Laboratory of Human Molecular Genetics, Faculty of Medicine, Sfax, 3029, Tunisia. lobna_h@lycos.com
authors.forename: Lobna
authors.initials: L
authors.lastname: Hadjkacem-Loukil
authors.forename: Imen
authors.initials: I
authors.lastname: Ayadi
authors.forename: Ali
authors.initials: A
authors.lastname: Bahloul
authors.forename: Hammadi
authors.initials: H
authors.lastname: Ayadi
authors.forename: Leila
authors.initials: L
authors.lastname: Ammar-Keskes
mesh_terms: D053713:Azoospermia / Q000235:genetics*; D041322:Chromosomes, Human, Y; D017353:Gene Deletion; D006801:Humans; D008297:Male; D016324:Sequence Tagged Sites; D014416:Tunisia