Transcriptional profiling of hexaploid wheat (Triticum aestivum L.) roots identifies novel, dehydration-responsive genes.
We used a long-oligonucleotide microarray to identify transcripts that increased or decreased in abundance in roots of dehydration-tolerant hexaploid bread wheat, in response to withholding of water. We observed that the major classes of dehydration-responsive genes (e.g. osmoprotectants, compatible solutes, proteases, glycosyltransferases/hydrolases, signal transducers components, ion transporters) were generally similar to those observed previously in other species and osmotic stresses. More specifically, we highlighted increases in transcript expression for specific genes including those putatively related to the synthesis of asparagine, trehalose, oligopeptide transporters, metal-binding proteins, the gamma-aminobutyric acid (GABA) shunt and transcription factors. Conversely, we noted a decrease in transcript abundance for diverse classes of glutathione and sulphur-related enzymes, specific amino acids, as well as MATE-efflux carrier proteins. From these data, we identified a novel, dehydration-induced putative AP2/ERF transcription factor, which we predict to function as a transcriptional repressor. We also identified a dehydration-induced 'little protein' (LitP; predicted mass: 8 kDa) that is highly conserved across spermatophytes. Using qRT-PCR, we compared the expression patterns of selected genes between two related wheat genotypes that differed in their susceptibility to dehydration, and confirmed that these novel genes were highly inducible by water limitation in both genotypes, although the magnitude of induction differed.
Mohammadi
Mohsen
M
Department of Biological Sciences, University of Alberta, Edmonton, Canada T6E 2L3.
Kav
Nat N V
NN
Deyholos
Michael K
MK
D000222:Adaptation, Physiological / Q000235:genetics*; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism; D020869:Gene Expression Profiling; D017343:Genes, Plant; D005978:Glutathione / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D018517:Plant Roots / Q000378:metabolism*; D011123:Polyploidy; D011134:Polysaccharides / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D014908:Triticum / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D014867:Water / Q000502:physiology*
D000596:Amino Acids; D026901:Membrane Transport Proteins; D011134:Polysaccharides; D014867:Water; D005978:Glutathione