Metabolism of reactive oxygen species in cotton cytoplasmic male sterility and its restoration.
To elucidate reactive oxygen species (ROS) metabolism of cotton cytoplasmic male sterility and the effects of restorer gene on the metabolism of ROS, the metabolism changes in the production and scavenging of ROS and gene expression related to ROS-scavenging enzymes were investigated in the anther mitochondria of CMS line, maintainer line and hybrid F(1). During the abortion preliminary stage (sporogenous cell division stage), anthers of CMS line had a little higher superoxide (O(2)(-)) production rate and hydrogen peroxide (H(2)O(2)) and malondialdehyde (MDA) contents than those of maintainer or hybrid F(1. )Simultaneously, a little higher ROS contents might serve as a signal to increase the activity of superoxide dismutase (SOD) in anthers of CMS line to reduce the ROS damage to the anther development. But at the abortion peak (pollen mother cell meiosis stage), anthers of CMS line had extraordinarily higher ROS contents and lower ROS-scavenging enzymic activities compared with the hybrid F(1), during which the ROS contents and ROS-scavenging enzymic activities in hybrid F(1) were approximate to those of maintainer line. The expression of Mn-sod and apx mRNA in anther of CMS line was obviously inhibited when ROS produced with a great deal during anther abortion, however the gene expression in hybrid F(1) kept normal with the maintainer. Excessive accumulation of O(2)(*-) , H(2)O(2 )and MDA, significant reduction of ROS-scavenging enzymic activities and lower gene expression level of ROS-scavenging enzyme were coinstantaneous with male cells death in anthers of CMS line. But when the restorer gene was transferred into CMS line, excessive production of ROS could be eliminated in the anthers of hybrid F(1). The restorer gene likely plays an important role in keeping the dynamic balance between the production and elimination of ROS.
Jiang
Peidong
P
Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China.
Zhang
Xiaoquan
X
Zhu
Yunguo
Y
Zhu
Wei
W
Xie
Haiyan
H
Wang
Xuede
X
D060387:Ascorbate Peroxidases; D016166:Free Radical Scavengers / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D003368:Gossypium / Q000235:genetics / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D010544:Peroxidases / Q000235:genetics / Q000378:metabolism; D051479:Plant Infertility; D018515:Plant Leaves / Q000378:metabolism; D011058:Pollen / Q000166:cytology / Q000196:embryology / Q000201:enzymology / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D014158:Transcription, Genetic
D016166:Free Radical Scavengers; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D013481:Superoxides; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D010544:Peroxidases; D060387:Ascorbate Peroxidases; D013482:Superoxide Dismutase