Rapid determination of polyprenylated xanthones in gamboge resin of Garcinia hanburyi by HPLC.
A rapid ion-pair HPLC method was developed and validated for the determination of eight polyprenylated xanthones including three pairs of epimers, namely morellic acid (MA), 30-hydroxygambogic acid (HGA), 30-hydroxyepigambogic acid (HEGA), isogambogic acid (IGA), epiisogambogic acid (EIGA), gambogenic acid (GNA), gambogic acid (GA), and epigambogic acid (EGA), in gamboge resin of Garcinia hanburyi. The separation was performed on a narrow bore C8 column with isocratic elution using a mixture of methanol-ACN-40 mM KH2PO4 buffer (37.5:37.5:25 v/v/v, containing 0.1% tetradecyltrimethylammonium bromide). The newly developed method was used to determine the contents of the eight compounds present in the gamboge. Results showed that GA and EGA are the dominant components of gamboge. The content ratio of each epimer pair remained constant, indicating that the content ratio of epimers can be used as a specific characteristic for the quality control of gamboge.
Song
Jing-Zheng
JZ
Chinese Medicine Laboratory, Hong Kong Jockey Club Institute of Chinese Medicine, Shatin, N. T., Hong Kong, China.
Yip
Yue-Keung
YK
Han
Quan-Bin
QB
Qiao
Chun-Feng
CF
Xu
Hong-Xi
HX
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D029760:Garcinia / Q000737:chemistry*; D015394:Molecular Structure; D012116:Resins, Plant / Q000032:analysis* / Q000737:chemistry; D044004:Xanthones / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification
D012116:Resins, Plant; D044004:Xanthones