Evaluation of three molecular assays for rapid identification of methicillin-resistant Staphylococcus aureus.
One home-developed assay and two commercial assays for the rapid identification of methicillin-resistant Staphylococcus aureus (MRSA) were compared by use of a collection of clinical isolates displaying highly diverse genetic backgrounds. Our results suggest that users of orfX-staphylococcal cassette chromosome mec-based assays should repeatedly monitor the local epidemiology to minimize the risks of detection bias and the omission of emerging MRSA clones.
Francois
Patrice
P
Department of Internal Medicine, Service of Infectious Diseases/Genomic Research Laboratory, University of Geneva Hospitals, CH-1211 Geneva 14, Switzerland. patrice.francois@genomic.ch
Bento
Manuela
M
Renzi
Gesuele
G
Harbarth
Stephan
S
Pittet
Didier
D
Schrenzel
Jacques
J
D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D006801:Humans; D016106:Methicillin Resistance; D009693:Nucleic Acid Hybridization / Q000379:methods*; D046915:Penicillin-Binding Proteins; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000145:classification* / Q000187:drug effects* / Q000235:genetics; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D046915:Penicillin-Binding Proteins; D011933:Reagent Kits, Diagnostic; C414317:SarH1 protein, Staphylococcus aureus; D014157:Transcription Factors; C487789:mecA protein, Staphylococcus aureus