title: Inhibition of severe acute respiratory syndrome-associated coronavirus infection by equine neutralizing antibody in golden Syrian hamsters.
abstract: Equine anti-severe acute respiratory syndrome-associated coronavirus F(ab')(2) has been verified to protect mice from infection with severe acute respiratory syndrome-associated coronavirus (SARS-CoV). However, before potential clinical application, the antibody needs to be tested in as many animal models as possible to ensure its safety and efficiency. In this study, after verification by various methods that the golden Syrian hamster constitutes a model susceptible to SARS-CoV infection, we confirmed that the antibody could protect animals completely from SARS-CoV infection in the preventive setting. More importantly, the antibody could reduce viral titers or copies by approximately 10(3)- to 10(4)-fold in animal lung after virus exposure, compared with negative control. These data provide further evidence to warrant clinical studies of this antibody in the treatment and prevention of SARS.
authors.affiliation: Department of Immunology, Institute of Microbiology Epidemiology, Academy of Military Medical Sciences, Beijing, People's Republic of China.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Zhao
authors.forename: Bing
authors.initials: B
authors.lastname: Ni
authors.forename: Haiyan
authors.initials: H
authors.lastname: Jiang
authors.forename: Deyan
authors.initials: D
authors.lastname: Luo
authors.forename: Marek
authors.initials: M
authors.lastname: Pacal
authors.forename: Lili
authors.initials: L
authors.lastname: Zhou
authors.forename: Liangyan
authors.initials: L
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Xing
authors.forename: Liangyan
authors.initials: L
authors.lastname: Zhang
authors.forename: Zhengcai
authors.initials: Z
authors.lastname: Jia
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Lin
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Jintao
authors.initials: J
authors.lastname: Li
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Liang
authors.forename: Xinfu
authors.initials: X
authors.lastname: Shi
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhao
authors.forename: Liyun
authors.initials: L
authors.lastname: Zhou
authors.forename: Yuzhang
authors.initials: Y
authors.lastname: Wu
authors.forename: Xiliang
authors.initials: X
authors.lastname: Wang
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D006224:Cricetinae; D006736:Horses; D007140:Immunoglobulin Fab Fragments / Q000276:immunology*; D008647:Mesocricetus; D009500:Neutralization Tests; D016133:Polymerase Chain Reaction; D045169:Severe Acute Respiratory Syndrome / Q000517:prevention & control*
chemical_list: D000914:Antibodies, Viral; D007140:Immunoglobulin Fab Fragments