title: Transforming growth factor-beta1 accelerates resorption of a calcium carbonate biomaterial in periodontal defects.
abstract: BACKGROUND
  In a previous study, recombinant human transforming growth factor-beta1 (rhTGF-beta(1)) in a calcium carbonate carrier was implanted into critical-size, supraalveolar periodontal defects under conditions for guided tissue regeneration (GTR) to study whether rhTGF-beta(1) would enhance or accelerate periodontal regeneration. The results showed minimal benefits of rhTGF-beta(1), and a clear account for this could not be offered. One potential cause may be that the rhTGF-beta(1) formulation was biologically inactive. Several growth or differentiation factors have been suggested to accelerate degradation of biomaterials used as carriers. The objective of this study was to evaluate possible activity of rhTGF-beta(1) on biodegradation of the calcium carbonate carrier.
  
  
  METHODS
  rhTGF-beta(1) in a putty-formulated particulate calcium carbonate carrier was implanted into critical-size, supraalveolar periodontal defects under conditions for GTR in five beagle dogs. Contralateral defects received the calcium carbonate carrier combined with GTR without rhTGF-beta(1) (control). The animals were euthanized at week 4 post-surgery and block biopsies of the defect sites were collected for histologic and histometric analysis. Radiographs were obtained at defect creation and weeks 2 and 4 after defect creation.
  
  
  RESULTS
  No statistically significant differences were observed in new bone formation (bone height and area) among the treatments. However, total residual carrier was significantly reduced in sites receiving rhTGF-beta(1) compared to control (P = 0.04). Similarly, carrier density was considerably reduced in sites receiving rhTGF-beta(1) compared to control; the difference was borderline statistically significant (P = 0.06).
  
  
  CONCLUSIONS
  Within the limitations of the study, it may be concluded that rhTGF-beta(1) accelerates biodegradation of a particulate calcium carbonate biomaterial, indicating a biologic activity of the rhTGF-beta(1) formulation apparently not encompassing enhanced or accelerated periodontal regeneration.
authors.affiliation: Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University, Seoul, Korea.
authors.forename: Ki-Tae
authors.initials: KT
authors.lastname: Koo
authors.forename: Cristiano
authors.initials: C
authors.lastname: Susin
authors.forename: Ulf M E
authors.initials: UM
authors.lastname: Wikesjö
authors.forename: Seong-Ho
authors.initials: SH
authors.lastname: Choi
authors.forename: Chong-Kwan
authors.initials: CK
authors.lastname: Kim
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001861:Bone Regeneration; D002119:Calcium Carbonate / Q000627:therapeutic use*; D004285:Dogs; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D008297:Male; D010510:Periodontal Diseases / Q000000981:diagnostic imaging / Q000601:surgery; D011859:Radiography; D011994:Recombinant Proteins / Q000627:therapeutic use; D053773:Transforming Growth Factor beta1 / Q000627:therapeutic use*
chemical_list: D011994:Recombinant Proteins; D053773:Transforming Growth Factor beta1; D002119:Calcium Carbonate