title: Nonradioactive detection of telomerase activity using the telomeric repeat amplification protocol.
abstract: The telomeric repeat amplification protocol (TRAP) is a two-step process for analyzing telomerase activity in cell or tissue extracts. Recent modifications of this sensitive assay include elimination of radioactivity by using a fluorescently labeled primer instead of a radiolabeled primer. In addition, the TRAP assay has been modified for real-time, quantitative PCR analysis. Here, we describe cost-effective procedures for detection of telomerase activity using a fluorescent-based assay as well as by using real-time PCR. These modified TRAP assays can be accomplished within 4 h (from lysis of samples to analysis of telomerase products).
authors.affiliation: Department of Medical and Molecular Genetics, Indiana University Cancer Center, Indiana University School of Medicine, Indianapolis, Indiana 46202-5251, USA. brherber@iupui.edu
authors.forename: Brittney-Shea
authors.initials: BS
authors.lastname: Herbert
authors.forename: Amelia E
authors.initials: AE
authors.lastname: Hochreiter
authors.forename: Woodring E
authors.initials: WE
authors.lastname: Wright
authors.forename: Jerry W
authors.initials: JW
authors.lastname: Shay
mesh_terms: D017931:DNA Primers; D005453:Fluorescence; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D019098:Telomerase / Q000032:analysis*; D016615:Telomere / Q000235:genetics*; D020079:Terminal Repeat Sequences / Q000235:genetics
chemical_list: D017931:DNA Primers; D019098:Telomerase