title: Development of a quality evaluation method for Fructus schisandrae by pressurized capillary electrochromatography.
abstract: A pressurized CEC (pCEC) method with postcolumn detection cell had been developed for quantifying the lignans from Fructus schisandrae extracts. The effects of different experimental conditions, such as the ACN content of the mobile phase, the concentration and pH of the buffer, the applied voltage, and the supplementary pressure were studied. Five lignans (schisandrin, gomisin A, schisantherin C, deoxyschizandrin, schisandrin B) were baseline separated using a mobile phase of ACN-phosphate buffer (pH 5.4; 5 mM) (40:60 v/v) under -4 kV applied voltage. The method showed the satisfactory retention time and peak area repeatability. The calibration curves were linear in the range 50.0-1000.0 microg/mL for schisandrin, 20.0-500.0 microg/mL for gomisin A, 10.0-200.0 microg/mL for schisantherin C, 20.0-500.0 microg/mL for deoxyschizandrin, and 20.0-500.0 microg/mL for schisandrin B. The correlation coefficients were between 0.9978 and 0.9989. With this pCEC system, fingerprints of F. schisandrae were preliminarily established to distinguish two members S. chinensis (Turcz.) Baill. and S. sphenanthera Rehd. Et Wils. of F. schisandrae by characteristic peaks, and evaluate the quality of various sources of raw materials by determining the contents of the five lignans.
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai, PR China.
authors.forename: Jiajing
authors.initials: J
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Chen
authors.forename: Mei
authors.initials: M
authors.lastname: Lin
authors.forename: Guorong
authors.initials: G
authors.lastname: Fan
authors.forename: Weiquan
authors.initials: W
authors.lastname: Zhao
authors.forename: Yutian
authors.initials: Y
authors.lastname: Wu
authors.forename: Chao
authors.initials: C
authors.lastname: Yan
authors.forename: Jianmin
authors.initials: J
authors.lastname: Wang
mesh_terms: D002021:Buffers; D053801:Capillary Electrochromatography / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D004563:Electrochemistry; D004867:Equipment Design; D006863:Hydrogen-Ion Concentration; D017705:Lignans / Q000032:analysis / Q000737:chemistry; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry*; D011312:Pressure; D011786:Quality Control; D032141:Schisandra / Q000737:chemistry*
chemical_list: D002021:Buffers; D017705:Lignans