Using interferon-alpha to block expression of cellular ribosome subunit S24 variant 2 in human fibroblasts inhibits translation of the poliovirus genome.
Studies about the proteins induced by interferon (IFN-)-alpha stimulation have provided some data on their mechanism of antiviral effect. These proteins were confirmed to contribute to antiviral functions. In this study, IFN-alpha stimulation of human fibroblasts was shown to induce the inhibition of S24 variant 2 (a structural component of the ribosomal small subunit) at the mRNA and protein levels, implying a possible antiviral mechanism for IFN-alpha in human fibroblasts. The delay of poliovirus replication by IFN-alpha was partially compensated for by S24 variant 2 expressed in pcDNA vector-transfected cells, and the interference RNA of S24 variant 2 was able to induce mimetically, to some extent, this poliovirus replication delay. These observations revealed that S24 variant 2 could be involved in the antiviral effects of IFN-alpha in human fibroblasts.
Liu
Longding
L
Institute of Medical Biology, Chinese Academy of Medical Sciences, Peking Union Medical College, Kunming, People's Republic of China.
Li
Pingzhong
P
Jiang
Li
L
Dong
Chenghong
C
Wang
Lichun
L
Che
Yanchun
Y
Zhao
Hongling
H
Dong
Shaozhong
S
Li
Qihan
Q
D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D005347:Fibroblasts / Q000821:virology; D016679:Genome, Viral; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology*; D017955:Poliovirus / Q000235:genetics*; D014176:Protein Biosynthesis / Q000187:drug effects*; D034622:RNA Interference; D012269:Ribosomal Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics
D000998:Antiviral Agents; D016898:Interferon-alpha; C489053:RPS24 protein, human; D012269:Ribosomal Proteins