title: Rapid DNA hybridization analysis using a PDMS microfluidic sensor and a molecular beacon.
abstract: A rapid DNA analysis has been developed based on a fluorescence intensity change of a molecular beacon in a PDMS microfluidic channel. Recently, we reported a new analytical method of DNA hybridization involving a PDMS microfluidic sensor using fluorescence energy transfer (FRET). However, there are some limitations in its application to real DNA samples because the target DNA must be labelled with a suitable fluorescent dye. To resolve this problem, we have developed a new DNA microfluidic sensor using a molecular beacon. By monitoring the change in the restored fluorescence intensity along the channel length, it is possible to rapidly detect any hybridization of the molecular beacon to the target DNA. In this case, the target DNA does not need to be labelled. Our experimental results demonstrate that this microfluidic sensor using a molecular beacon is a promising diagnostic tool for rapid DNA hybridization analysis.
authors.affiliation: Department of Applied Chemistry, Hanyang University, South Korea.
authors.forename: Sungyong
authors.initials: S
authors.lastname: Kim
authors.forename: Lingxin
authors.initials: L
authors.lastname: Chen
authors.forename: Sangyeop
authors.initials: S
authors.lastname: Lee
authors.forename: Gi Hun
authors.initials: GH
authors.lastname: Seong
authors.forename: Jaebum
authors.initials: J
authors.lastname: Choo
authors.forename: Eun Kyu
authors.initials: EK
authors.lastname: Lee
authors.forename: Chil-Hwan
authors.initials: CH
authors.lastname: Oh
authors.forename: Sanghoon
authors.initials: S
authors.lastname: Lee
mesh_terms: D015342:DNA Probes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D009693:Nucleic Acid Hybridization / Q000379:methods; D009841:Oligonucleotides / Q000737:chemistry*
chemical_list: D015342:DNA Probes; D005456:Fluorescent Dyes; D009841:Oligonucleotides