Using cadmium telluride quantum dots as a proton flux sensor and applying to detect H9 avian influenza virus.
Semiconductor nanocrystals, often known as quantum dots, have been used extensively for a wide range of applications in bioimaging and biosensing. In this article, we report that the pH-sensitive cadmium telluride (CdTe) quantum dots (QDs) were used as a proton sensor to detect proton flux that was driven by ATP synthesis in chromatophores. To confirm that these QD-labeled chromatophores were responding to proton flux pumping driven by ATP synthesis, N,N'-dicyclohexylcarbodiimide (DCCD) was used as an inhibitor of ATPase activity. Furthermore, we applied the QD-labeled chromatophores as a virus detector to detect the H9 avian influenza virus based on antibody-antigen reaction. The results showed that this QD virus detector could be a new virus-detecting device.
Yun
Zhang
Z
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
Zhengtao
Deng
D
Jiachang
Yue
Y
Fangqiong
Tang
T
Qun
Wei
W
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D020130:Bacterial Chromatophores / Q000378:metabolism; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D019187:Cadmium Compounds / Q000737:chemistry*; D002642:Chick Embryo; D004024:Dicyclohexylcarbodiimide / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D053127:Influenza A Virus, H9N2 Subtype / Q000276:immunology / Q000302:isolation & purification*; D017494:Proton Pumps / Q000737:chemistry*; D045663:Quantum Dots; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D013691:Tellurium / Q000737:chemistry*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D019187:Cadmium Compounds; D017494:Proton Pumps; D004024:Dicyclohexylcarbodiimide; D013691:Tellurium; C028337:cadmium telluride