[Beneficial actions of lipoic acid in cerebral cortex homogenates in rats during lipopolysaccharide-induced oxidative stress].
The aim of his study was to assess the effect of lipoic acid (LA) on lipid peroxidation, hydrogen peroxide(H2O2) concentration, sulfhryl group (-SH) contents in the cerebral cortex homogenates in the early stage of oxidative stress.


METHODS
Experiments were performed on male rats Wistar 300-320 g body weight. The animals were divided into four groups consisting of eight animals each. Control group received saline alone into the tail vein (i.v.). The second group received LA (60 mg/kg b.w., i.v.). The third group received LPS (30 mg/kg b.w., i.v.). The fourth group received LPS (30 mg/kg b.w., i.v.) and after 0.5 h received LA (60 mg/kg b.w., i.v.). In the homogenates from cerebral cortex were measured thiobarbituric acid reactants (TBARS) as indicator lipid peroxidation, H2O2 concentration and -SH groups contents.


RESULTS
The administration of LA after LPS-induced oxidative stress caused a significant decrease in TBARS and H2O2 concentration 5.38 + 0.084 micromol and 0.028 + 0.002 micromol, respectively) compared with the group treated with LPS (6.96 + 0.063 micromol and 0.198 + 0.003 micromol, respectively). Treatment of LPS-injected rats with LA caused increase in -SH groups (p < 0.05).


CONCLUSIONS
The early administration of LA a significant suppressed symptoms of oxidative stress-induced LPS, expressed as a decrease in lipid peroxidation process, H2O2 generation and increase in concentration of protein containing thiol groups in the cerebral cortex homogenates.
Goraca
Anna
A
Department of Experimental and Clinical Physiology, Medical University of Lodz, Poland. agoraca@zdn.am.lodz.pl
D000818:Animals; D002540:Cerebral Cortex / Q000737:chemistry / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000032:analysis / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013438:Sulfhydryl Compounds / Q000032:analysis / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis* / Q000378:metabolism; D008063:Thioctic Acid / Q000494:pharmacology*
D008070:Lipopolysaccharides; D013438:Sulfhydryl Compounds; D017392:Thiobarbituric Acid Reactive Substances; D008063:Thioctic Acid; D006861:Hydrogen Peroxide