Sensitive whole-cell biosensor suitable for detecting a variety of N-acyl homoserine lactones in intact rhizosphere microbial communities.
To investigate quorum sensing in rhizosphere soil, a whole-cell biosensor, Agrobacterium tumefaciens(pAHL-Ice), was constructed. The biosensor responded to all N-acyl homoserine lactones (AHLs) tested, except C(4) homoserine lactone, with a minimum detection limit of 10(-12) M, as well as to both exogenously added AHLs and AHL-producing bacterial strains in soil. This highly sensitive biosensor reveals for the first time the increased AHL availability in intact rhizosphere microbial communities compared to that in bulk soil.
DeAngelis
Kristen M
KM
Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA.
Firestone
Mary K
MK
Lindow
Steven E
SE
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000032:analysis; D016960:Agrobacterium tumefaciens / Q000737:chemistry / Q000235:genetics; D018407:Bacterial Physiological Phenomena; D015374:Biosensing Techniques / Q000379:methods*; D005822:Genetic Vectors; D010957:Plasmids / Q000235:genetics; D053038:Quorum Sensing / Q000502:physiology*; D012988:Soil Microbiology
C088386:homoserine lactone; D015107:4-Butyrolactone