Determination of plant resistance to carbamate herbicidal compounds inhibiting cell division and early growth by seed and plantlets bioassays.
Herbicide-resistant plants can be generated by either traditional breeding procedures or genetic engineering. Analyses of plant responses to a newly developed herbicide or the tolerance level of a newly developed plant line to a given herbicide are based on various bioassays. Here, we describe several methods for quantitative measurements of plants' responses to propham application, as a model herbicide of the carbamate family. Dose-response assays include seed germination and analyses of shoot and root elongation on paper. To better reflect the natural interaction between the plant, the soil and the herbicide, a protocol for germination and root elongation on sand is described. Finally, a more sensitive bioassay is based on plant growth on agar medium. The described protocols are simple, reproducible and can be easily adopted for a variety of plant species and for various herbicides. Plants' response to a given herbicide can be determined within a few weeks.
Weiss
Yael
Y
The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University, Rehovot 76100, Israel.
Rubin
Baruch
B
Shulman
Avidor
A
Ben Shir
Irina
I
Keinan
Ehud
E
Wolf
Shmuel
S
D001681:Biological Assay / Q000379:methods*; D002455:Cell Division / Q000187:drug effects; D018525:Germination / Q000187:drug effects*; D053209:Herbicide Resistance; D006540:Herbicides / Q000494:pharmacology*; D019684:Magnoliopsida / Q000187:drug effects*; D048448:Phenylcarbamates / Q000494:pharmacology*; D018517:Plant Roots / Q000254:growth & development; D036226:Seedlings / Q000187:drug effects*
D006540:Herbicides; D048448:Phenylcarbamates; C100175:propham