title: A paramagnetic nanoprobe to detect tumor cell death using magnetic resonance imaging.
abstract: A 110 kDa (ca. 5 nm in diameter) bivalent paramagnetic nanoprobe for detecting cell death using magnetic resonance imaging (MRI) is described, in which two biotinylated C2A domains of the protein synaptotagmin-I were complexed with a single avidin molecule, which had been labeled with gadolinium chelates. This nanoprobe bound with high affinity and specificity to the phosphatidylserine exposed by dying cells and was demonstrated to allow MRI detection of apoptotic tumor cells in vitro.
authors.affiliation: Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1GA, U.K.
authors.forename: André A
authors.initials: AA
authors.lastname: Neves
authors.forename: Anant S
authors.initials: AS
authors.lastname: Krishnan
authors.forename: Mikko I
authors.initials: MI
authors.lastname: Kettunen
authors.forename: De-En
authors.initials: DE
authors.lastname: Hu
authors.forename: Maaike M de
authors.initials: MM
authors.lastname: Backer
authors.forename: Bazbek
authors.initials: B
authors.lastname: Davletov
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Brindle
mesh_terms: D000818:Animals; D001360:Avidin / Q000737:chemistry; D016923:Cell Death; D045744:Cell Line, Tumor; D005682:Gadolinium / Q000737:chemistry; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D015335:Molecular Probes; D009369:Neoplasms / Q000473:pathology*; D050863:Synaptotagmin I / Q000737:chemistry
chemical_list: D015335:Molecular Probes; D050863:Synaptotagmin I; D001360:Avidin; D005682:Gadolinium