title: [Effect of 5 drugs with different antitumor mechanism on tumor-formation potential of rat hepatocarcinoma cells and mesenchymal stem cells].
abstract: OBJECTIVE
  To study the association of drug sensitivity of rat hepatocarcinoma cells and bone marrow mesenchymal stem cells (MSCs) to 5 antitumor drug with different antitumor mechanisms with tumorigenicity of the hepatocarcinoma cells in nude mice.
  
  
  METHODS
  Primary liver carcinoma was induced with diethylnitrosamine in rats, and the tumor cells and MSCs were obtained from 10 of the rats. The inhibition ratio of the hepatocarcinoma cells and MSCs following treatment with the 5 drugs were measured by MTT assay. The weight of the tumor in nude mice resulting from the injection of the isolated tumor cells treated with the 5 anticancer drugs was measured 6 weeks after implantation. For each anticancer drug, the difference in the inhibition ratio of the anticancer drugs against the hepatocarcinoma cells and MSCs, and he correlation of the inhibition ratio of the anticancer drugs with implanted tumor weight were analyzed.
  
  
  RESULTS
  No correlation was found between the inhibition ratio of the 5 anticancer drugs against the hepatocarcinoma cells and the tumor weight of nude mice, but a significant negative correlation was identified between the inhibition ratio of the MSCs and the tumor weight.
  
  
  CONCLUSIONS
  MSCs have similar drug resistance mechanism to the tumor stem cells. The inhibition ratio of the anticancer drugs against the MSCs can help evaluate the invasion potential of hepatocarcinoma cells.
authors.affiliation: Oncology Center, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Min
authors.initials: M
authors.lastname: Luo
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Wen-qing
authors.initials: WQ
authors.lastname: Zhang
authors.forename: Ji-ren
authors.initials: JR
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D008114:Liver Neoplasms, Experimental / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D014411:Neoplastic Stem Cells / Q000187:drug effects* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays / Q000379:methods
chemical_list: D000970:Antineoplastic Agents