Adjuvant effects of crystal proteins from a Mexican strain of Bacillus thuringiensis on the mouse humoral response.
In this study, we determined the adjuvant effects of the crystal (Cry) proteins, p130, p98, and p64-62, on the immune response of mice to both sheep red blood cells (SRBC) and ovalbumin (OVA). The administration of p130, p98, and p64-62 Cry proteins to Balb/c mice induced a significant (p<0.01) increase in the production of anti-SRBC antibody-secreting cells (ASC). The p64-62 Cry proteins demonstrated the best ability to induce the production of IgA and IgG antibodies to SRBC (p<0.05), and IgM, IgA, and IgG antibodies to OVA (p<0.05). Additionally, Cry proteins did not produce any side effects associated with their administration to Balb/c mice. We suggest the potential use of the p64-62 Cry proteins as adjuvants for the administration of heterologous antigens.
Román Calderón
Máximo E
ME
Laboratorio de Inmunología y Virología, Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Apartado Postal 46 "F", C.P. 66450, Nuevo León, Mexico.
Alcocer González
Juan M
JM
Franco Molina
Moisés A
MA
Tamez Guerra
Reyes S
RS
Rodríguez Padilla
Cristina
C
D000276:Adjuvants, Immunologic / Q000008:administration & dosage* / Q000302:isolation & purification; D000818:Animals; D000941:Antigens / Q000008:administration & dosage; D001413:Bacillus thuringiensis / Q000276:immunology* / Q000302:isolation & purification; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000008:administration & dosage* / Q000276:immunology* / Q000302:isolation & purification; D001427:Bacterial Toxins / Q000008:administration & dosage* / Q000276:immunology* / Q000302:isolation & purification; D002417:Cattle; D004731:Endotoxins / Q000008:administration & dosage* / Q000276:immunology* / Q000302:isolation & purification; D004912:Erythrocytes / Q000276:immunology; D006460:Hemolysin Proteins / Q000008:administration & dosage* / Q000276:immunology* / Q000302:isolation & purification; D007070:Immunoglobulin A / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000276:immunology; D008800:Mexico; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000008:administration & dosage / Q000276:immunology; D012756:Sheep
D000276:Adjuvants, Immunologic; D000941:Antigens; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008070:Lipopolysaccharides; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D012710:Serum Albumin, Bovine; D010047:Ovalbumin