[Effect of platelet CD42a modification by mPEG-SPA with different molecular masses].
OBJECTIVE
To observe the effect platelet antigen modification by mPEG-SPA with different molecular masses.


METHODS
Platelet CD42a was modified by 5 kD and 20 kD mPEG-SPA, respectively, and the fluorescence intensity of CD42a was detect by flow cytometry and the three-dimensional structure of CD42a simulated to analyze the distribution of lysine in CD42a molecule.


RESULTS
After platelet CD42a modification by 5 kD and 20 kD mPEG-SPA, the fluorescence intensity of CD42a decreased sharply by 85.54% and 88.65%, respectively, and multiple lysine regions were identified on the surface of CD42a molecule.


CONCLUSIONS
Both 5 kD and 20 kD mPEG-SPA allow useful modification of platelet CD42a, but 20 kD mPEG-SPA is more advantageous than 5 kD mPEG-SPA.
Zhang
Yin-ze
YZ
Shenzhen Institute of Transfusion Medicine, Shenzhen 518035, China. zyz200157@sohu.com
Xiong
Wen
W
Li
Zhen
Z
Shao
Chao-peng
CP
Li
Tian-jun
TJ
Zhao
Feng
F
Yang
Bao-cheng
BC
D001792:Blood Platelets / Q000737:chemistry*; D006801:Humans; D008970:Molecular Weight; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D013388:Succinimides / Q000737:chemistry*
D019038:Platelet Glycoprotein GPIb-IX Complex; D013388:Succinimides; D011092:Polyethylene Glycols; C028210:monomethoxypolyethylene glycol