title: Antiapoptotic activity of bovine herpesvirus type-1 (BHV-1) UL14 protein.
abstract: Viruses have evolved different strategies to interfere with apoptotic pathways in order to halt cellular responses to infection. One previous study showed that transient transfection of bovine herpesvirus type-1 (BHV-1) UL14 protein is efficient in protecting Madin Darby kidney (MDBK) and human chronic myelogenous leukemia (K562) cells from sorbitol-induced apoptosis. This protein corresponds to a putative protein of BHV-1, which shares aminoacid sequence with a part of the peptide-binding domain conserved in human heat shock protein (HSP70) family. The pBK-CMV-UL14 plasmid transfected MDBK cells treated with sorbitol did not show caspase-3 and caspase-9 activation with respect to non-transfected MDBK cells (UL14 negative). Furthermore, we report that the expression of the full length sequence of BHV-1 UL14 is evident after 7 h of infection of BHV-1 on MDBK cells which were then treated with sorbitol. These results indicate that UL14 gene product has important implications to enhance cell survival in response to apoptotic stimuli.
authors.affiliation: Department of Pathology and Animal Health, Infectious Diseases, Medicine Veterinary School, University of Naples Federico II, Via F. Delpino 1, 80137 Naples, Italy. luisa.demartino@unina.it
authors.forename: L
authors.initials: L
authors.lastname: De Martino
authors.forename: G
authors.initials: G
authors.lastname: Marfe
authors.forename: M Irno
authors.initials: MI
authors.lastname: Consalvo
authors.forename: C
authors.initials: C
authors.lastname: Di Stefano
authors.forename: U
authors.initials: U
authors.lastname: Pagnini
authors.forename: P
authors.initials: P
authors.lastname: Sinibaldi-Salimei
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D002417:Cattle; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D007242:Herpesvirus 1, Bovine / Q000378:metabolism*; D006801:Humans; D013012:Sorbitol; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C402639:UL14 protein, Human herpesvirus 1; D014764:Viral Proteins; D013012:Sorbitol; D053148:Caspase 3; D053453:Caspase 9