[Activity of antioxidant enzymes in patients with liver cirrhosis].
The aim of our studies was the estimation of activities of antioxidant enzymes in patients with liver cirrhosis. We investigated activities of superoxide dismutases (CuZnSOD, MnSOD), catalase (CAT), selenium dependent GSH peroxidase (Se-GSH-Px), selenium independent GSH peroxidase (non-Se-GSH-Px), GSH-S-transferase (GST), GSH reductase (GSHR) and the level ofreduced gutathione (GSH) in cirrhotic and healthy liver tissues. The activities of CuZnSOD, MnSOD, CAT and GSH-dependent enzymes (except GSHR) were found to be lower in cirrhotic tissue compared to healthy liver. Those changes were associated with decrease of GSH level in cirrhotic tissue compared with control liver tissue. Our results show that antioxidant barrier in liver cirrhosis is impaired. It is associated with decrease of glutathione level and changes of activities of antioxidant enzymes (SOD, CAT, GSHPx, GST, GSHR) in liver cirrhosis compared with healthy liver.
Czeczot
Hanna
H
Katedry i Zakładu Biochemii Akademii Medycznej w Warszawie. hanna.czeczot@wp.pl
Scibior
Dorota
D
Skrzycki
Michał
M
Podsiad
Małgorzata
M
D000328:Adult; D000975:Antioxidants / Q000032:analysis*; D002374:Catalase / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000201:enzymology* / Q000473:pathology / Q000502:physiology / Q000601:surgery; D008103:Liver Cirrhosis / Q000201:enzymology* / Q000473:pathology / Q000601:surgery*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D012016:Reference Values; D013482:Superoxide Dismutase / Q000378:metabolism; D000099156:Superoxide Dismutase 2
D000975:Antioxidants; D002374:Catalase; D005978:Glutathione; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D013482:Superoxide Dismutase; D000099156:Superoxide Dismutase 2