Genotype differentiating RT-PCR and sandwich ELISA: handy tools in epidemiological investigation of foot and mouth disease.
Years of molecular epidemiological surveillance has revealed co-circulation of two antigenically divergent genotypes of foot and mouth disease virus serotype A in India. Genotype differentiating RT-PCR and sandwich ELISA were developed as fast, cost-effective and user-friendly alternatives to 1D region based phylogeny for detection and differentiation of genotype VI and VII. The RT-PCR assay targeting 1D region was found to be more sensitive and authentic in distinguishing genotypes than sandwich ELISA. These assays promise to be reliable tools in the epidemiological investigation of foot and mouth disease in the country.
Mohapatra
Jajati Keshari
JK
Project Directorate on Foot-and-Mouth Disease, Indian Veterinary Research Institute Campus, Mukteswar-Kumaon, Nainital 263138, Uttaranchal, India. jajati4@email2me.net
Subramaniam
Saravanan
S
Tosh
Chakradhar
C
Hemadri
Divakar
D
Sanyal
Aniket
A
Periyasamy
Tamilselvan Ramasamy
TR
Rasool
Thaha Jamal
TJ
D000818:Animals; D000956:Antigens, Viral; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D005536:Foot-and-Mouth Disease / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D005838:Genotype; D007194:India; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012703:Serotyping
D000956:Antigens, Viral