[Experssion of human glutathione-S-transferase A1 in CHO cells].
OBJECTIVE
To establish the Chinese Hamster Ovary (CHO) expression system for the human glutathione-S-transferase (GST) A1.


METHODS
CHO cells was transfected with recombinant vector pDNA3.1- GSTA1 by lipofectamineTM 2000. The expression of selected CHO-GSTA1 in G418-resistant clones were determined by PCR, RT-PCR and Western blot. CHO-GSTA1 high-expressed clone was cultured to collect supernatant and activity was assayed by U-2001 ultraviolet spectrophotometer.


RESULTS
CHO-GSTA1 was obtained by PCR, RT-PCR and Western blot. The expressed protein of GSTA1 had biological activity, it was 35 mmol/(mg min pro).


CONCLUSIONS
The CHO-GSTA1 was constructed and for research purification protein and candidate strains of detoxicitic mechanism of chemical toxins and carcinogenesis.
Chen
Pingping
P
Department of Nutrition and Food Hygiene, Public Health College, Zhengzhou University, Zhengzhou 450052, China. chenpingping871@sohu.com
Wang
Qi
Q
Li
Wenjie
W
Cui
Lingling
L
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D005982:Glutathione Transferase / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection
D011994:Recombinant Proteins; C484555:GSTA1 protein, human; D005982:Glutathione Transferase