title: [Protective effect of donator liver ischemic preconditioning on posttransplant liver graft function in Chinese miniature pigs].
abstract: OBJECTIVE
  To investigate the protective effect of the ischemia preconditioning of the donor liver on posttransplant liver graft function in Chinese miniature pigs.
  
  
  METHODS
  Twenty-five partially inbred Chinese miniature pigs were randomized into three groups, namely the normal control group, the ischemia-reperfusion group and ischemic preconditioning group. Biopsies of the liver graft were performed to analyze HSP70 expression by means of immunoblotting, and the changes of serum AST/ALT levels were assayed using an automated biochemical analyzer. Histopathological assessment was carried out to identify the hepatocyte injury using optical and transmission electron microscopy.
  
  
  RESULTS
  Ischemia preconditioning resulted in a notable increase in HSP70 expression and milder injury of the hepatocyte microstructure, whereas ischemia-reperfusion caused a significant increase of serum transaminases level (P<0.01) with declined HSP70 expression and obvious microstructural changes of the liver tissue.
  
  
  CONCLUSIONS
  Ischemic preconditioning can produce obvious protective effects on the donor liver, and positively regulates the expression of shock protein.
authors.affiliation: Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Ming-xin
authors.initials: MX
authors.lastname: Pan
authors.forename: Fan-dong
authors.initials: FD
authors.lastname: Kong
authors.forename: Zhi-feng
authors.initials: ZF
authors.lastname: Wu
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Hai-lan
authors.initials: HL
authors.lastname: Wang
authors.forename: Ai-hui
authors.initials: AH
authors.lastname: Li
authors.forename: Jin-ming
authors.initials: JM
authors.lastname: Xie
authors.forename: Yi
authors.initials: Y
authors.lastname: Gao
mesh_terms: D000818:Animals; D005260:Female; D006085:Graft Survival / Q000502:physiology*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D019194:Ischemic Preconditioning; D008099:Liver / Q000098:blood supply* / Q000378:metabolism / Q000503:physiopathology; D016031:Liver Transplantation; D008297:Male; D011897:Random Allocation; D013552:Swine; D013556:Swine, Miniature
chemical_list: D018840:HSP70 Heat-Shock Proteins