[Effects of (60)Co gamma rays on the biological behaviour of lung fibroblasts in C57BL/6J and C3H/HeN mice].
OBJECTIVE
To investigate the effects of (60)Co gamma rays on the biological behaviour of lung fibroblasts (LFs) in C57BL/6J and C3H/HeN mice.


METHODS
LFs in C57BL/6J and C3H/HeN mice were isolated, cultured and irradiated by 2, 4, 6 and 8 Gy (60)Co gamma rays. The proliferative activity and cell cycle were detected by MTT colorimetry, AgNOR staining and fluorescence-activated cell sorting(FACS). Then the expressions of alpha-smooth muscle actin (a-SMA), matrix metal proteinase (MMP-1) and tissue inhibitor of metalloproteinase (TIMP-1) in LFs were observed by immunocytochemical staining.


RESULTS
The proliferation of LFs in C57BL/6J mice was not promoted by 2, 4, 6 and 8 Gy (60)Co gamma rays, but the number of aneuploid cells increased. MMP-1 was weak positive. The expression of a-SMA and TIMP-1 was enhanced by (60)Co gamma rays. The proliferation of LFs in C3H/HeN mice was not promoted by 2, 4, 6 and 8 Gy (60)Co gamma rays either, but the number of cells in G2-M phase increased. The expression of MMP-1 and TIMP-1 was enhanced, but the expression of a-SMA was decreased by (60)Co gamma rays.


CONCLUSIONS
The biological behaviours of the irradiated LFs in C57BL/6J and C3H/HeN mice are different. LFs in C57BL/6J mice can be pactivatedq. Our study will provide a celluar basis for the researches into the C57BL/6J mice which are prone to radiation pulmonary fibrosis (RPF).
Liu
Ying
Y
Beijing Institute of Radiation Medicine, Beijing 100850, China.
Li
Yang
Y
Peng
Rui-Yun
RY
Wang
Shui-Ming
SM
Gao
Ya-Bing
YB
Ma
Jun-Jie
JJ
Song
Liang-Wen
LW
D000199:Actins / Q000378:metabolism; D000818:Animals; D002453:Cell Cycle / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects*; D005434:Flow Cytometry; D005720:Gamma Rays; D015870:Gene Expression / Q000528:radiation effects; D007150:Immunohistochemistry; D008168:Lung / Q000166:cytology*; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism
D000199:Actins; D019715:Tissue Inhibitor of Metalloproteinase-1; D020781:Matrix Metalloproteinase 1