Characterization of immobilized nuclease P1.
To improve the efficiency of the use of nuclease P1, enzyme immobilization technology was applied using nuclease P1. Characterization of immobilized nuclease P1 on different supports was studied. The results showed that the optimum pH and temperature of nuclease P1 immobilized via different supports were enhanced. The immobilized enzyme was obviously stable when stored for long periods and was reusable. The best results were obtained when nuclease P1 was immobilized on chitosan nanoparticles. The nanoparticles were applied to protect the activity of nuclease P1 and improved enzyme activity by 13.17% over that of free nuclease P1 at the same conditions. The Michaelis constant Km and Vmax were determined for free and immobilized enzyme as well.
Ying
Quo-Qing
QQ
College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China.
Shi
Lu-E
LE
Zhang
Xiao-Ying
XY
Chen
Wei
W
Yi
Yu
Y
D002482:Cellulose; D048271:Chitosan; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D053758:Nanoparticles; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism*; D013696:Temperature
D004800:Enzymes, Immobilized; D002482:Cellulose; D048271:Chitosan; D015719:Single-Strand Specific DNA and RNA Endonucleases