title: Genotyping of Chlamydia trachomatis from endocervical specimens in Brazil.
abstract: BACKGROUND
  There is no data concerning genotyping of Chlamydia trachomatis from Brazilian samples.
  
  
  OBJECTIVE
  To characterize the genotype of C. trachomatis detected in women assisted at a STD public clinic and establish the prevalence of this infection in that population.
  
  
  METHODS
  Endocervical samples of a group of 100 women were tested for chlamydial infection with PCR directed to C. trachomatis cryptic plasmid. Genotyping of positive samples were done after omp1 amplification and sequencing.
  
  
  RESULTS
  The overall prevalence of C. trachomatis infection was 19%, with the highest prevalence in women between 15 and 25 years old (68.4%). Four genotypes were found associated with endocervical infections: D, E, F, and K. Sequence analysis revealed a coinfection of genotypes D and E in 1 woman.
  
  
  CONCLUSIONS
  To our knowledge this is the first study to characterize Brazilian C. trachomatis endocervical samples and Brazilian C. trachomatis genotype coinfection. Our results also emphasize the importance of routine diagnosis of C. trachomatis for the control of this STD.
authors.affiliation: Departamento de Microbiologia, Universidade Federal de Minas Gerais, Brasil.
authors.forename: Haleta E
authors.initials: HE
authors.lastname: Lima
authors.forename: Milena B
authors.initials: MB
authors.lastname: Oliveira
authors.forename: Brenda G
authors.initials: BG
authors.lastname: Valente
authors.forename: Daniela A F
authors.initials: DA
authors.lastname: Afonso
authors.forename: Wanderson D
authors.initials: WD
authors.lastname: Darocha
authors.forename: Maria Carmo M
authors.initials: MC
authors.lastname: Souza
authors.forename: Túlio C
authors.initials: TC
authors.lastname: Alvim
authors.forename: Edel F
authors.initials: EF
authors.lastname: Barbosa-Stancioli
authors.forename: Fátima Soares Motta
authors.initials: FS
authors.lastname: Noronha
mesh_terms: D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D001938:Brazil / Q000453:epidemiology; D002690:Chlamydia Infections / Q000453:epidemiology* / Q000382:microbiology* / Q000517:prevention & control; D002692:Chlamydia trachomatis / Q000235:genetics* / Q000302:isolation & purification; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015995:Prevalence; D014626:Vaginal Smears
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial