5-bromodeoxyuridine increases transient expression of ectopic genes in human cells.
Ectopic genes transferred to cells are temporally expressed, although this phenomenon has not yet been well characterized. We found that 5-bromodeoxyuridine dramatically increased transient expression of ectopic genes in human cells. This effect was elicited by adding 5-bromodeoxyuridine prior to or after transfection. No promoter specificity was observed. Real time PCR analysis showed an approximately 2-fold increase in mRNA levels. Since 5-bromodeoxyuridine decondenses heterochromatin and changes the nuclear envelope, these changes might affect transcriptional and post-transcriptional events in the gene expression of plasmids.
Endoh
Morio
M
Kihara Institute for Biological Research and Graduate School of Integrated Science, Yokohama City University, Yokohama, Japan.
Miki
Kensuke
K
Hossain
Mohammad Nazir
MN
Fujii
Michihiko
M
Ayusawa
Dai
D
D000199:Actins / Q000235:genetics; D000963:Antimetabolites / Q000494:pharmacology*; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects*; D017930:Genes, Reporter / Q000187:drug effects; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D006801:Humans; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000187:drug effects; D014746:Vimentin / Q000235:genetics
D000199:Actins; D000963:Antimetabolites; D014746:Vimentin; D004247:DNA; D008156:Luciferases; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D001973:Bromodeoxyuridine