Basic protocols for Drosophila S2 cell line: maintenance and transfection.
Cells in culture have been increasingly employed in the dissection of intracellular processes. They are generally easier to handle than the organism of study and certainly less complex, which facilitates testing for specific functions and protein-protein interactions. This chapter will describe the extremely simple steps required to keep a healthy S2 cell culture going.
Ceriani
M Fernanda
MF
Department Behavioral Genetics, Fundación Instituto Leloir, Buenos Aires, Argentina.
D000818:Animals; D002122:Calcium Chloride; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D015925:Cryopreservation; D003470:Culture Media / Q000737:chemistry; D004330:Drosophila / Q000166:cytology* / Q000235:genetics*; D010714:Phosphatidylethanolamines; D014162:Transfection / Q000379:methods*
D003470:Culture Media; D010714:Phosphatidylethanolamines; C020888:1,2-dielaidoylphosphatidylethanolamine; D002122:Calcium Chloride