title: 'Gate effect' in templated polyacrylamide membranes influences the electrotransport of proteins and finds applications in proteome analysis.
abstract: Templating is an effective way for the structural modifications of a material and hence for altering its functional properties. Here protein imprinting was exploited to alter polymeric polyacrylamide (PAA) membranes. The sieving properties and selection abilities of the material formed were evaluated by studying the electrically driven transport of various proteins across templated PAA membranes. The sieving properties correlated with the templating process and depended on the quantity of template used during the polymerisation. For 1 mg/mL protein-templated membranes a 'gate effect' was shown, which induced a preferential migration of the template and of similar-size proteins. Such template preferential electrotransport was exploited for the selective removal of certain proteins in biological fluids prior to proteome analysis (depletion of albumin from human serum); the efficiency of the removal was demonstrated by analysing the serum proteome by two-dimensional electrophoresis experiments.
authors.affiliation: Department of Science and Technology, University of Verona, Strada le Grazie 15, 37134 Verona, Italy. alessandra.bossi@univr.it
authors.forename: Alessandra
authors.initials: A
authors.lastname: Bossi
authors.forename: Matteo
authors.initials: M
authors.lastname: Andreoli
authors.forename: Francesca
authors.initials: F
authors.lastname: Bonini
authors.forename: Sergey
authors.initials: S
authors.lastname: Piletsky
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D008567:Membranes, Artificial; D011506:Proteins / Q000737:chemistry*; D020543:Proteome; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D008567:Membranes, Artificial; D011506:Proteins; D020543:Proteome; D000099166:Polyacrylamides