title: A new highly effective anticysticercosis vaccine expressed in transgenic papaya.
abstract: The use of transgenic plants as new antigen-delivery systems for subunit vaccines has been increasingly explored. We herein report progress toward a papaya-based vaccine against cysticercosis. Synthetic peptides (KETc1, KETc12, KETc7) were successfully expressed in 19 different transgenic papaya clones and found to be immunogenic. Complete protection against cysticercosis was induced with the soluble extract of the clones that expressed the higher levels of transcripts in up to 90% of the immunized mice. This study represents a key step towards the development of a more effective, sustainable and affordable oral subunit vaccine against human and pig cysticercosis.
authors.affiliation: Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México, D.F. 04510, México.
authors.forename: Marisela
authors.initials: M
authors.lastname: Hernández
authors.forename: José Luis
authors.initials: JL
authors.lastname: Cabrera-Ponce
authors.forename: Gladis
authors.initials: G
authors.lastname: Fragoso
authors.forename: Fernando
authors.initials: F
authors.lastname: López-Casillas
authors.forename: Arturo
authors.initials: A
authors.lastname: Guevara-García
authors.forename: Gabriela
authors.initials: G
authors.lastname: Rosas
authors.forename: Claudia
authors.initials: C
authors.lastname: León-Ramírez
authors.forename: Patricia
authors.initials: P
authors.lastname: Juárez
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Sánchez-García
authors.forename: Jaquelynne
authors.initials: J
authors.lastname: Cervantes
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Acero
authors.forename: Andrea
authors.initials: A
authors.lastname: Toledo
authors.forename: Carmen
authors.initials: C
authors.lastname: Cruz
authors.forename: Rafael
authors.initials: R
authors.lastname: Bojalil
authors.forename: Luis
authors.initials: L
authors.lastname: Herrera-Estrella
authors.forename: Edda
authors.initials: E
authors.lastname: Sciutto
mesh_terms: D000818:Animals; D029441:Carica / Q000235:genetics*; D003551:Cysticercosis / Q000276:immunology* / Q000517:prevention & control*; D003552:Cysticercus / Q000254:growth & development / Q000276:immunology*; D005260:Female; D008018:Life Cycle Stages; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D010529:Peritoneal Cavity / Q000469:parasitology; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D022223:Vaccines, Subunit / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic