Watching neuronal circuit dynamics through functional multineuron calcium imaging (fMCI).
Functional multineuron calcium imaging (fMCI) is a large-scale optical recording technique that monitors the spatiotemporal pattern of action potentials, all at once, from large neuron populations. fMCI has unique advantages, including: (i) simultaneous recording from >1000 neurons in a wide area, (ii) single-cell resolution, (iii) identifiable location of neurons and (iv) detection of non-active neurons during the observation period. We review herein the principle, history, utility and limitations of fMCI.
Takahashi
Naoya
N
Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan.
Sasaki
Takuya
T
Usami
Atsushi
A
Matsuki
Norio
N
Ikegaya
Yuji
Y
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003952:Diagnostic Imaging / Q000266:history / Q000379:methods*; D049673:History, 20th Century; D049674:History, 21st Century; D009415:Nerve Net / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D013569:Synapses / Q000502:physiology
D002118:Calcium