title: In-gel digestion for mass spectrometric characterization of proteins and proteomes.
abstract: In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass spectrometry (MS)-driven proteomics. The 10-year-old recipe by Shevchenko et al. has been optimized to increase the speed and sensitivity of analysis. The protocol is for the in-gel digestion of both silver and Coomassie-stained protein spots or bands and can be followed by MALDI-MS or LC-MS/MS analysis to identify proteins at sensitivities better than a few femtomoles of protein starting material.
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany. shevchenko@mpi-cbg.de
authors.forename: Andrej
authors.initials: A
authors.lastname: Shevchenko
authors.forename: Henrik
authors.initials: H
authors.lastname: Tomas
authors.forename: Jan
authors.initials: J
authors.lastname: Havlis
authors.forename: Jesper V
authors.initials: JV
authors.lastname: Olsen
authors.forename: Matthias
authors.initials: M
authors.lastname: Mann
mesh_terms: D004586:Electrophoresis; D013058:Mass Spectrometry; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*
chemical_list: D011506:Proteins