title: Epizootiological survey of Trichinella spp. infection in carnivores, rodents and insectivores in Hokkaido, Japan.
abstract: In order to evaluate the present epidemiological situation of Trichinella infection in wild animals in Hokkaido, Japan, red foxes (Vulpes vulpes), raccoon dogs (Nyctereutes procyonoides) , brown bears (Ursus arctos) , martens (Martes melampus), rodents and insectivores captured in Hokkaido were examined for muscle larvae by the artificial digestion method from 2000 to 2006. Foxes (44/319, 13.8%), raccoon dogs (6/77, 7.8%) and brown bears (4/126, 3.2%) were found to be infected with Trichinella larvae and all other animal species evaluated were negative. Multiplex PCR and DNA sequencing revealed that larvae from a fox captured in Otofuke, in south-eastern Hokkaido, were T. nativa, and larvae from 27 animals including 21 foxes, 2 raccoon dogs and 4 brown bears captured in western Hokkaido were Trichinella T9.
authors.affiliation: Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, 060-0818, Japan.
authors.forename: Yuta
authors.initials: Y
authors.lastname: Kanai
authors.forename: Takashi
authors.initials: T
authors.lastname: Inoue
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Mano
authors.forename: Nariaki
authors.initials: N
authors.lastname: Nonaka
authors.forename: Ken
authors.initials: K
authors.lastname: Katakura
authors.forename: Yuzaburo
authors.initials: Y
authors.lastname: Oku
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000469:parasitology*; D002335:Carnivora; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D007312:Eulipotyphla; D005260:Female; D007564:Japan / Q000453:epidemiology; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D012377:Rodentia; D017422:Sequence Analysis, DNA; D014234:Trichinella / Q000235:genetics / Q000302:isolation & purification*; D014235:Trichinellosis / Q000453:epidemiology* / Q000469:parasitology / Q000662:veterinary*
chemical_list: D018509:DNA, Helminth