title: Actin binding and proline rich motifs of CR16 play redundant role in growth of vrp1Delta cells.
abstract: CR16, (Glucocorticoid-regulated) belongs to the verprolin family of proteins which are characterized by the presence of a V domain (verprolin) at the N-terminal. Expression of CR16 suppressed the growth and endocytosis defect of vrp1Delta strain without correcting the actin patch polarization defect. The V domain of CR16 is critical for suppression of the growth defect of vrp1Delta strain but not for localisation to cortical actin patches. Mutations in the actin binding motif alone did not abolish the activity of CR16 but the mutations in combination with deletion of N-terminal proline rich motif abolished the ability of CR16 to suppress the growth defect. This suggests that the V domain of CR16 has two functionally redundant motifs and either one of these motifs is sufficient for suppressing the growth defect of vrp1Delta strain. This is in contrast to the observation that both WIP and WIRE require the actin binding motif for their activity.
authors.affiliation: School of Biological Sciences, Nanyang Technological University, Singapore 637551, Republic of Singapore.
authors.forename: Lei
authors.initials: L
authors.lastname: Meng
authors.forename: Rajamuthiah
authors.initials: R
authors.lastname: Rajmohan
authors.forename: Shangjuan
authors.initials: S
authors.lastname: Yu
authors.forename: Thirumaran
authors.initials: T
authors.lastname: Thanabalu
mesh_terms: D000199:Actins / Q000378:metabolism*; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001665:Binding Sites; D049109:Cell Proliferation / Q000187:drug effects; D003598:Cytoskeletal Proteins; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000378:metabolism*; D010750:Phosphoproteins / Q000737:chemistry* / Q000378:metabolism*; D011392:Proline / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D015003:Yeasts / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D000199:Actins; D003598:Cytoskeletal Proteins; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; C105364:Wipf3 protein, rat; D011392:Proline