A major zebrafish polymorphism resource for genetic mapping.
We have identified 645,088 candidate polymorphisms in zebrafish and observe a single nucleotide polymorphism (SNP) validation rate of 71% to 86%, improving with polymorphism confidence score. Variant sites are non-random, with an excess of specific novel T- and A-rich motifs. We positioned half of the polymorphisms on zebrafish genetic and physical maps as a resource for positional cloning. We further demonstrate bulked segregant analysis using the anchored SNPs as a method for high-throughput genetic mapping in zebrafish.
Bradley
Kevin M
KM
Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232-0275, USA. Kevin.bradley@vanderbilt.edu
Elmore
J Bradford
JB
Breyer
Joan P
JP
Yaspan
Brian L
BL
Jessen
Jason R
JR
Knapik
Ela W
EW
Smith
Jeffrey R
JR
D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D003001:Cloning, Molecular; D008040:Genetic Linkage; D005819:Genetic Markers; D020641:Polymorphism, Single Nucleotide; D015027:Zebrafish / Q000235:genetics*
D005819:Genetic Markers