Cytotoxicity analysis of EDTA and citric acid applied on murine resident macrophages culture.
OBJECTIVE
To assess the ex vivo cytotoxicity of EDTA and citric acid solutions on macrophages.


METHODS
The cytotoxicity of 17% EDTA and 15% citric acid was evaluated on murine macrophage cultures using MTT-Tetrazolium method [3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide]. A total of 5 x 10(5) cells were plated in medium culture with 17% EDTA or 15% citric acid. Fresh medium was used as a control. Toxicity values were analysed statistically by anova and Tukey's test (P<0.05) at short (0, 6, 12, 24 h) and medium periods (1, 3, 5, 7 days), using ELISA absorbance.


RESULTS
On the short term, both EDTA (0.253 nm) and citric acid (0.260 nm) exhibited cytotoxic effects on macrophage cultures (P<0.05). On the medium term, statistical differences were observed (P<0.05) between the groups. EDTA (0.158 nm) and citric acid (0.219 nm) were cytotoxic when compared with the control group; EDTA-reduced macrophage viability significantly more than citric acid (P<0.05).


CONCLUSIONS
Both EDTA and citric acid had effects on macrophages cells ex vivo, but citric acid was less toxic in periods from 1 to 7 days of use.
Amaral
K F
KF
Department of Endodontics, Dental School, University of São Paulo, São Paulo, Brazil.
Rogero
M M
MM
Fock
R A
RA
Borelli
P
P
Gavini
G
G
D000818:Animals; D019343:Citric Acid / Q000633:toxicity*; D004492:Edetic Acid / Q000633:toxicity*; D008264:Macrophages / Q000187:drug effects*; D008297:Male; D051379:Mice; D012388:Root Canal Irrigants / Q000633:toxicity*; D013997:Time Factors
D012388:Root Canal Irrigants; D019343:Citric Acid; D004492:Edetic Acid