[Effect of anatoxin-A on calcium-mediate signal system of brain tissue in mice].
OBJECTIVE
To explore the mechanism of central neurotoxity induced by anatoxin-A (ANTX-A) from the calcium-mediate signal system.


METHODS
Mice were administered by i.p. at different doses of ANTX-A for one month, 0.9% of sodium chloride solution was used as the control. Using fluorescence method and colorimetry method, the intracellular calcium concentration and the activity of calcineurin (CaN, PP2B) and ATPase in brain were determined.


RESULTS
Calcium concentrations in brain were increased in 12.5, 50 and 200 microg/kg bw groups compared with the control group, the difference had statistical significance (P < 0.01). The activity of PP2B of brain in 200 microg/kg bw group was enhanced compared with the control group (P < 0.05). In all ANTX-A treated groups, the activity of Na+ -K+ -ATPase of brain in mice had no obvious changes, but the activity of Ca2+ -ATPase in 50 and 200 microg/kg bw groups were significantly reduced compared with the control group ( P < 0.05).


CONCLUSIONS
It is suggested that calcium-mediate signal system could have important regulative action in the central neurotoxity induced by antoxin-A.
Zhang
Zhihong
Z
Department of Environmental Health, School of Public Health, Fudan University, Shanghai 200032, China. zzh8759@yahoo.com.cn
Qu
Weidong
W
Jiang
Songhui
S
Zhu
Huigang
H
D017033:Anabaena; D000818:Animals; D001921:Brain / Q000378:metabolism*; D019703:Calcineurin / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D000087522:Cyanobacteria Toxins; D005260:Female; D008297:Male; D051379:Mice; D011897:Random Allocation; D014326:Tropanes / Q000633:toxicity*
D000087522:Cyanobacteria Toxins; D014326:Tropanes; C509783:anatoxin a; D019703:Calcineurin; D000252:Calcium-Transporting ATPases; D002118:Calcium