title: Extraction of plant RNA.
abstract: This protocol details an RNA preparation for medium-scale, high-purity RNA production from higher plants. It uses hot acid phenol with standard sodium acetate ethanol precipitation and is suitable for producing RNA for both Northern blotting and enzyme-based downstream applications such as RT-PCR and microarray studies.
authors.affiliation: Department of Biology, University of Leicester, UK.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Salter
authors.forename: Helen E
authors.initials: HE
authors.lastname: Conlon
mesh_terms: D017360:Arabidopsis / Q000737:chemistry / Q000235:genetics; D015152:Blotting, Northern; D011232:Chemical Precipitation; D000431:Ethanol; D020411:Oligonucleotide Array Sequence Analysis; D019800:Phenol; D018749:RNA, Plant / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019346:Sodium Acetate
chemical_list: D018749:RNA, Plant; D019800:Phenol; D000431:Ethanol; D019346:Sodium Acetate