title: Mutagenic effect of freezing on mitochondrial DNA of Saccharomyces cerevisiae.
abstract: Although suggested in some studies, the mutagenic effect of freezing has not been proved by induction and isolation of mutants. Using a well-defined genetic model, we supply in this communication evidence for the mutagenic effect of freezing on mitochondrial DNA (mtDNA) of the yeast Saccharomyces cerevisiae. The cooling for 2 h at +4 degrees C, followed by freezing for 1 h at -10 degrees C and 16 h at -20 degrees C resulted in induction of respiratory mutations. The immediate freezing in liquid nitrogen was without mutagenic effect. The study of the stepwise procedure showed that the induction of respiratory mutants takes place during the freezing at -10 and -20 degrees C of cells pre-cooled at +4 degrees C. The genetic crosses of freeze-induced mutants evidenced their mitochondrial rho- origin. The freeze-induced rho- mutants are most likely free of simultaneous nuclear mutations. The extracellular presence of cryoprotectants did not prevent the mutagenic effect of freezing while accumulation of cryoprotectors inside cells completely escaped mtDNA from cryodamage. Although the results obtained favor the notion that the mutagenic effect of freezing on yeast mtDNA is due to formation and growth of intracellular ice crystals, other reasons, such as impairment of mtDNA replication or elevated levels of ROS production are discussed as possible explanations of the mutagenic effect of freezing. It is concluded that: (i) freezing can be used as a method for isolation of mitochondrial mutants in S. cerevisiae and (ii) given the substantial development in cryopreservation of cells and tissues, special precautions should be made to avoid mtDNA damage during the cryopreservation procedures.
authors.affiliation: Institute of Cryobiology and Food Technology, 53A Cherni Vrah Blvd, 1407 Sofia, Bulgaria.
authors.forename: T
authors.initials: T
authors.lastname: Stoycheva
authors.forename: P
authors.initials: P
authors.lastname: Venkov
authors.forename: Ts
authors.initials: Ts
authors.lastname: Tsvetkov
mesh_terms: D003451:Cryoprotective Agents / Q000494:pharmacology; D004271:DNA, Fungal / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D004579:Electron Transport / Q000235:genetics; D005615:Freezing; D005990:Glycerol / Q000494:pharmacology; D000432:Methanol / Q000494:pharmacology; D016296:Mutagenesis / Q000187:drug effects; D010085:Oxidative Phosphorylation; D012234:Rho Factor / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D014199:Trehalose / Q000494:pharmacology
chemical_list: D003451:Cryoprotective Agents; D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D012234:Rho Factor; D014199:Trehalose; D005990:Glycerol; D000432:Methanol