title: Diarylheptanoids from Alnus hirsuta inhibit the NF-kB activation and NO and TNF-alpha production.
abstract: Six diarylheptanoids (1-6) from the stem bark of Alnus hirsuta were investigated for their inhibitory activity against LPS-induced NF-kB activation and NO and TNF-alpha production. Among them, compounds 2, 3, and 6 displayed inhibitory activity against NF-kB activation and NO and TNF-alpha production with IC50 values of 9.2-9.9 microM, 18.2-19.3 microM, and 22.3-23.7 microM, respectively, in RAW264.7 cells. Three active compounds had no significant cytotoxicity in RAW264.7 cells at their effective concentrations. This is the first report of NF-kB-inhibitory activity of these compounds and supports the pharmacological use of A. hirsuta, which has been employed as a herbal medicine for the treatment of inflammatory diseases.
authors.affiliation: College of Pharmacy, Chungnam National University, Korea.
authors.forename: WenYi
authors.initials: W
authors.lastname: Jin
authors.forename: Xing Fu
authors.initials: XF
authors.lastname: Cai
authors.forename: MinKyun
authors.initials: M
authors.lastname: Na
authors.forename: Jung Joon
authors.initials: JJ
authors.lastname: Lee
authors.forename: KiHwan
authors.initials: K
authors.lastname: Bae
mesh_terms: D029661:Alnus / Q000737:chemistry*; D000818:Animals; D002460:Cell Line; D036381:Diarylheptanoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D000432:Methanol / Q000737:chemistry; D051379:Mice; D015394:Molecular Structure; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D009569:Nitric Oxide / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000096:biosynthesis
chemical_list: D036381:Diarylheptanoids; D016328:NF-kappa B; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D000432:Methanol