RNA in situ hybridizations on Drosophila whole mounts.
RNA in situ hybridization is a commonly used technique to achieve spatiotemporal detection of transcripts in tissues. This chapter gives an overview of novel techniques using fluorescent dyes, signal amplification methods, and confocal microscopy in regard to chronobiological applications on Drosophila adult brains.
Wülbeck
Corinna
C
Institut für Zoologie, Universität Regensburg, Germany.
Helfrich-Förster
Charlotte
C
D000818:Animals; D000906:Antibodies; D001483:Base Sequence; D001921:Brain / Q000502:physiology; D002909:Chronobiology Phenomena / Q000235:genetics* / Q000502:physiology*; D004247:DNA / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics* / Q000502:physiology*; D019286:Endopeptidase K; D005456:Fluorescent Dyes; D017344:Genes, Insect; D005822:Genetic Vectors; D006241:Haptens; D017403:In Situ Hybridization / Q000379:methods*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics*; D015347:RNA Probes; D014158:Transcription, Genetic
D000906:Antibodies; D005456:Fluorescent Dyes; D006241:Haptens; D015347:RNA Probes; D012313:RNA; D004247:DNA; D019286:Endopeptidase K