title: Microtiter plate transformation using the LiAc/SS carrier DNA/PEG method.
abstract: Here, we describe a protocol that has been adapted for the transformation of yeast cells in 96-well microtiter plates. This protocol can be tailored for multiple applications and is suitable for high-throughput applications. It can be completed in 2-3 h, once the yeast cells have been grown depending on the heat shock used.
authors.affiliation: Department of Biochemistry and Medical Genetics, University of Manitoba, T250-770 Bannatyne Ave., Winnipeg, Manitoba R3E 0W3, Canada.
authors.forename: R Daniel
authors.initials: RD
authors.lastname: Gietz
authors.forename: Robert H
authors.initials: RH
authors.lastname: Schiestl
mesh_terms: D000085:Acetates; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D004277:DNA, Single-Stranded / Q000235:genetics; D006358:Hot Temperature; D011092:Polyethylene Glycols; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D014170:Transformation, Genetic
chemical_list: D000085:Acetates; D004277:DNA, Single-Stranded; D011092:Polyethylene Glycols; C488804:lithium acetate