Anti-BDCA-4 (neuropilin-1) antibody can suppress virus-induced IFN-alpha production of plasmacytoid dendritic cells.
Plasmacytoid dendritic cells (PDC) in human blood are the main source of virus-induced interferon (IFN)-alpha. They exhibit a lineage-negative phenotype but all express BDCA-4, which is homologous to the neuronal receptor neuropilin-1. Specific staining with anti-BDCA-4 antibody is used for positive isolation of PDC from blood by magnetic cells sorting. Here, it is demonstrated that these positively selected PDC showed reduced or completely abolished IFN-alpha release compared to unstained PDC, which were negatively selected by magnetic depletion of lineage-positive blood mononuclear cells. In addition, treatment of these unstained PDC with anti-BDCA-4 mAb also resulted in at least two-fold lower or reduced virus-induced IFN-alpha production. It is shown that the antibody not only affects cell survival or block virus attachment but also reduces IFN-alpha release induced by non-viral CpG oligodeoxynucleotides. In conclusion, data suggest an immunoregulatory role for BDCA-4 on PDC as demonstrated for IFN-alpha response to virus.
Grage-Griebenow
Evelin
E
Experimental Pneumology, Ruhr-University Bochum, Bochum, Germany.
Löseke
Stefan
S
Kauth
Marion
M
Gehlhar
Kirsten
K
Zawatzky
Rainer
R
Bufe
Albrecht
A
D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D002469:Cell Separation; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology* / Q000821:virology*; D005434:Flow Cytometry; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000235:genetics; D039942:Neuropilin-1 / Q000276:immunology*; D010641:Phenotype; D052736:Rous sarcoma virus / Q000187:drug effects* / Q000502:physiology*; D014158:Transcription, Genetic / Q000187:drug effects; D053585:Virus Attachment / Q000187:drug effects
D000911:Antibodies, Monoclonal; D039942:Neuropilin-1; D007371:Interferon-gamma