Nanomechanotransduction and interphase nuclear organization influence on genomic control.
The ability of cells to alter their genomic regulation in response to mechanical conditioning or through changes in morphology and the organization of the interphase nuclei are key questions in cell biology. Here, two nanotopographies have been used as a model surfaces to change cell morphology in order to investigate spatial genomic changes within the nuclei of fibroblasts. Initially, centromeres for chromosome pairs were labeled and the average distance on different substrates calculated. Further to this, Affymetrix whole genome GeneChips were used to rank genomic changes in response to topography and plot the whereabouts on the chromosomes these changes were occurring. It was seen that as cell spreading was changed, so were the positions along the chromosomes that gene regulations were being observed. We hypothesize that as changes in cell and thus nuclear morphology occur, that this may alter the probability of transcription through opening or closing areas of the chromosomes to transcription factors.
Dalby
Matthew J
MJ
Centre for Cell Engineering, Joseph Black Building, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, G12 8QQ, Scotland, UK. m.dalby@bio.gla.ac.uk
Gadegaard
Nikolaj
N
Herzyk
Pawel
P
Sutherland
Duncan
D
Agheli
Hossein
H
Wilkinson
Chris D W
CD
Curtis
Adam S G
AS
D001710:Biotin / Q000378:metabolism; D018929:Cell Culture Techniques; D002461:Cell Line, Transformed; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D002503:Centromere / Q000378:metabolism / Q000648:ultrastructure; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003102:Colloids; D004595:Electroplating; D005347:Fibroblasts / Q000648:ultrastructure; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D015894:Genome, Human; D006801:Humans; D007399:Interphase / Q000235:genetics*; D040542:Mechanotransduction, Cellular / Q000235:genetics*; D036103:Nanotechnology / Q000379:methods*; D009532:Nickel / Q000737:chemistry; D020411:Oligonucleotide Array Sequence Analysis; D011095:Polyethylenes / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D019904:Polymethyl Methacrylate / Q000737:chemistry; D011419:Propidium / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012825:Silicon / Q000737:chemistry; D013379:Substrate Specificity; D013451:Sulfonic Acids / Q000737:chemistry; D019098:Telomerase / Q000235:genetics / Q000378:metabolism; D014867:Water / Q000737:chemistry
D020099:Coated Materials, Biocompatible; D003102:Colloids; D005456:Fluorescent Dyes; D011095:Polyethylenes; D011108:Polymers; D000644:Quaternary Ammonium Compounds; D013451:Sulfonic Acids; D014867:Water; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride; D011419:Propidium; D001710:Biotin; D009532:Nickel; D019904:Polymethyl Methacrylate; D019098:Telomerase; D016650:Fluorescein-5-isothiocyanate; D012825:Silicon; C077114:styrenesulfonic acid polymer