Enhancement of proteolysis through the silica-gel-derived microfluidic reactor.
An on-chip enzymatic reactor providing rapid protein digestion is presented. Trypsin-embedding stationary phase within the microchannel has been prepared by the sol-gel method. Such a microfluidic reactor has been used for low-level protein digestion at 16 fmol per analysis. The analytical potential of the microreactor combined with the strong cation exchange and RPLC ESI-MS/MS for the identification of real samples from the cytoplasma of the human liver tissue has been demonstrated.
Liu
Yun
Y
Department of Chemistry and Institute of Biomedical Sciences, Fudan University, Shanghai, PR China.
Qu
Haiyun
H
Xue
Yan
Y
Wu
Zhonglin
Z
Yang
Pengyuan
P
Liu
Baohong
B
D003593:Cytoplasm / Q000737:chemistry; D006801:Humans; D008099:Liver / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D010447:Peptide Hydrolases / Q000378:metabolism*; D058428:Silica Gel; D012822:Silicon Dioxide; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D014357:Trypsin / Q000378:metabolism
D058428:Silica Gel; D012822:Silicon Dioxide; D010447:Peptide Hydrolases; D014357:Trypsin