HIV type 1 drug resistance among naive patients from Venezuela.
In this study, we characterize proviral DNA of 20 HIV-1 asymptomatic antiretroviral-naive patients from Venezuela in env, gag, and pol genes regions. Results from both env/gag HMA subtyping and phylogenetic analysis of pol partial sequences led to the description of clade B in all cases. Nevertheless, the high prevalence of polymorphisms was particularly evident among the protease sequences. A 10% prevalence of major resistance mutations to RTIs was found. Our data also suggested that the protease polymorphisms I62T and V77T could be considered as molecular markers of the subtype B local epidemic. In addition, we show how proviral DNA can be used as a reliable tool to follow trends of resistance mutation transmission.
Bouchard
Morella
M
Immunology Institute, Central University of Venezuela, Caracas, Venezuela.
Masquelier
Bernard
B
Moreno
Melcenia
M
Deibis
Leopoldo
L
De Pérez
Gloria Echeverría
GE
Fleury
Herve
H
Castro
Erika
E
D004252:DNA Mutational Analysis; D024921:Drug Resistance, Multiple, Viral / Q000235:genetics*; D005260:Female; D015658:HIV Infections / Q000145:classification / Q000188:drug therapy* / Q000453:epidemiology; D016333:HIV Protease / Q000145:classification / Q000235:genetics; D054303:HIV Reverse Transcriptase / Q000145:classification / Q000235:genetics; D015497:HIV-1 / Q000145:classification / Q000187:drug effects*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D014687:Venezuela / Q000453:epidemiology
D054303:HIV Reverse Transcriptase; D016333:HIV Protease