Evaluation of the resistance of blood plasma to oxidative stress by oxidizability of proteins and lipids during metal-catalyzed oxidation.
A new approach for the evaluation of oxidizability of proteins and lipids in the same sample of blood plasma was proposed. We tested a method for evaluation of metal-catalyzed oxidation of fibrinogen by the formation of bityrosine cross-links during oxidation detected by the increase in fluorescence at 415 nm. A correlation was revealed between parameters of oxidizability estimated by this marker and carbonyl derivatives (dinitrophenylhydrazine method). Oxidizability of total proteins from whole plasma was compared with oxidizability of plasma lipids (marker malonic dialdehyde). Study of these parameters in patients with coronary heart disease showed that the proposed experimental approach allows us to divide the sample into several subgroups differing in the resistance to oxidative stress. These data can be used for diagnostic and prognostic purposes.
Bekman
E M
EM
Institute of Physicochemical Medicine, Russian Ministry of Health, Moscow.
Baranova
O A
OA
Gubareva
E V
EV
Shulenina
L V
LV
Moskvina
S N
SN
Danilogorskaya
Yu A
YA
Azizova
O A
OA
D002021:Buffers; D002384:Catalysis; D002614:Chelating Agents / Q000494:pharmacology; D003300:Copper / Q000378:metabolism / Q000494:pharmacology; D003432:Cross-Linking Reagents / Q000378:metabolism; D003676:Deferoxamine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005069:Evaluation Studies as Topic; D005340:Fibrinogen / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000378:metabolism / Q000494:pharmacology; D050356:Lipid Metabolism; D008315:Malondialdehyde / Q000097:blood*; D008670:Metals / Q000378:metabolism / Q000494:pharmacology*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010949:Plasma / Q000378:metabolism*; D050050:Protein Carbonylation / Q000187:drug effects; D011506:Proteins / Q000378:metabolism*; D012965:Sodium Chloride / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013997:Time Factors
D002021:Buffers; D002614:Chelating Agents; D003432:Cross-Linking Reagents; D008670:Metals; D011506:Proteins; D012965:Sodium Chloride; D008315:Malondialdehyde; D003300:Copper; D005340:Fibrinogen; D007501:Iron; D003676:Deferoxamine