title: Prostaglandin E2 inhibits Paracoccidioides brasiliensis killing by human monocytes.
abstract: Human monocytes lacked fungicidal activity against high virulence strain of Paracoccidioides brasiliensis, even after IFN-gamma activation. However, monocytes treated with indomethacin exhibited an effective killing against this fungus, suggesting a role of prostaglandin E2 (PGE2) in the inhibition process. Thus, the purpose of this work was to determine whether the effect of PGE2 in fungicidal activity was related with decrease on H(2)O(2) release, the metabolite involved in P. brasiliensis killing, and changes in the levels of TNF-alpha, IL-6 and IL-10. Human monocytes challenged with the fungus produced high PGE2 levels, which in turn inhibited the fungicidal activity of these cells by reducing H(2)O(2) and TNF-alpha production.
authors.affiliation: Department of Microbiology and Immunology, Institute of Biosciences, São Paulo State University - UNESP, Botucatu, CEP: 18618-000 Sao Paulo, Brazil.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Bordon
authors.forename: Luciane Alarcão
authors.initials: LA
authors.lastname: Dias-Melicio
authors.forename: Michele Janegitz
authors.initials: MJ
authors.lastname: Acorci
authors.forename: Sueli Aparecida
authors.initials: SA
authors.lastname: Calvi
authors.forename: Maria Terezinha
authors.initials: MT
authors.lastname: Serrão Peraçoli
authors.forename: Angela Maria
authors.initials: AM
authors.lastname: Victoriano de Campos Soares
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D015232:Dinoprostone / Q000378:metabolism*; D015536:Down-Regulation; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D016753:Interleukin-10 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism*; D010228:Paracoccidioides / Q000378:metabolism*; D010229:Paracoccidioidomycosis / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D006861:Hydrogen Peroxide; D015232:Dinoprostone