Dead-time optimized time-correlated photon counting instrument with synchronized, independent timing channels.
Time-correlated single photon counting is a powerful method for sensitive time-resolved fluorescence measurements down to the single molecule level. The method is based on the precisely timed registration of single photons of a fluorescence signal. Historically, its primary goal was the determination of fluorescence lifetimes upon optical excitation by a short light pulse. This goal is still important today and therefore has a strong influence on instrument design. However, modifications and extensions of the early designs allow for the recovery of much more information from the detected photons and enable entirely new applications. Here, we present a new instrument that captures single photon events on multiple synchronized channels with picosecond resolution and over virtually unlimited time spans. This is achieved by means of crystal-locked time digitizers with high resolution and very short dead time. Subsequent event processing in programmable logic permits classical histogramming as well as time tagging of individual photons and their streaming to the host computer. Through the latter, any algorithms and methods for the analysis of fluorescence dynamics can be implemented either in real time or offline. Instrument test results from single molecule applications will be presented.
Wahl
Michael
M
PicoQuant GmbH, Rudower Chaussee 29, D-12489 Berlin, Germany. wahl@pq.fta-berlin.de
Rahn
Hans-Jürgen
HJ
Gregor
Ingo
I
Erdmann
Rainer
R
Enderlein
Jörg
J
D017785:Photons; D011874:Radiometry / Q000295:instrumentation*; D013997:Time Factors