Effect of copper on acid phosphatase activity in yeast Yarrowia lipolytica.
Acid phosphatase (APase) activity of the yeast Yarrowia lipolytica increased with increasing Cu2+ concentrations in the medium. Furthermore, the enzyme in soluble form was stimulated in vitro by Cu2+, Co2+, Ni2+, Mn2+ and Mg2+ and inhibited by Ag+ and Cd2+. The most effective ion was Cu2+, especially for the enzyme from cultures in medium containing Cu2+, whereas APase activity in wall-bound fragments was only slightly activated by Cu2+. The content of cellular phosphate involving polyphosphate was decreased by adding Cu2+, regardless of whether or not the medium was rich in inorganic phosphate. Overproduction of the enzyme stimulated by Cu2+ might depend on derepression of the gene encoding the APase isozyme.
Ito
Hiroyasu
H
Department of Biology, Faculty of Science, Ehime University, Matsuyama, 790-8577, Japan.
Inouhe
Masahiro
M
Tohoyama
Hiroshi
H
Joho
Masanori
M
D000135:Acid Phosphatase / Q000187:drug effects / Q000378:metabolism*; D002463:Cell Membrane Permeability; D003300:Copper / Q000494:pharmacology*; D005656:Fungal Proteins / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D016296:Mutagenesis; D025062:Yarrowia / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development
D005656:Fungal Proteins; D003300:Copper; D000135:Acid Phosphatase