title: [Effects of streptokinase and hyperbaric oxygen therapy on venous occlusion after limb reimplantation in rats].
abstract: OBJECTIVE
  The effectiveness of streptokinase and hyperbaric oxygen therapy on venous occlusion after limb reimplantation was tested in rats.
  
  
  METHODS
  Amputation with preservation of vessels and nerves of the right hind limb was carried out in 140 rats. Groups MG0, MG1, MG2, MG3 and MG4 were submitted to 0, 1, 2, 3 and 4 hours of venous occlusion. MG3 was elected as control for the experimental groups. Groups EG1 and EG2 were submitted to 3 hours of venous occlusion and were treated with streptokinase and hyperbaric oxygen therapy. Limbs were observed for 7 days and their mortality and survival rates were studied.
  
  
  RESULTS
  Trans-operatory mortality rates in groups MG0, MG1, MG2, MG3 and MG4 were 0, 10, 15, 30 and 60% respectively and the postoperative mortality rates were 5; 11.1; 11.7; 14.2 and 100% respectively. The limb survival rates were 100%, 87.5%, 80% and 66.67% respectively and 76.9% and 100% in EG1 and EG2. Model groups were statistically different, except for MG1 and MG2 in trans-operatory mortality rates. There were no statistical differences in postoperative mortality rates between model groups except for MG3 and MG4. Model groups were statistically different, with the exception of MG1 and MG2, in limb survival rates. EG1 and MG3 showed no statistical difference in limb survival and EG2 had a better limb survival than MG3.
  
  
  CONCLUSIONS
  Results suggest that the administration of streptokinase does not change effects of venous occlusion and that hyperbaric oxygen therapy may decrease the effects of venous occlusion in limbs.
authors.affiliation: Disciplina de Cirurgia Plástica, Laboratório de Cirurgia Plástica e Microcirurgia da FMUSP, Faculdade de Medicina, Universidade de São Paulo, Brazil.
authors.forename: Marcelo Sacramento
authors.initials: MS
authors.lastname: Cunha
authors.forename: José Carlos Faes
authors.initials: JC
authors.lastname: da Silva
authors.forename: Hugo Alberto
authors.initials: HA
authors.lastname: Nakamoto
authors.forename: Klaus Werner
authors.initials: KW
authors.lastname: Fels
authors.forename: Marcus Castro
authors.initials: MC
authors.lastname: Ferreira
mesh_terms: D000818:Animals; D016009:Chi-Square Distribution; D005121:Extremities / Q000098:blood supply* / Q000601:surgery; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D006931:Hyperbaric Oxygenation; D007430:Intraoperative Care; D007511:Ischemia / Q000188:drug therapy* / Q000401:mortality; D008297:Male; D023421:Models, Animal; D011184:Postoperative Period; D051381:Rats; D017208:Rats, Wistar; D012092:Replantation / Q000401:mortality; D013300:Streptokinase / Q000494:pharmacology*; D015996:Survival Rate; D013997:Time Factors
chemical_list: D005343:Fibrinolytic Agents; D013300:Streptokinase