Upregulation of heme oxygenase and collagen type III in the rat bladder after partial bladder outlet obstruction.
The objective of the study was to evaluate possible changes of the gene expression and localization of the enzymes, heme oxygenase and nitric oxide synthase (NOS), with reference to increase of collagen type III in response to the partial obstruction of the bladder. Following initial obstruction, whole rat bladders were removed for real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. Real-time RT-PCR demonstrated significantly enhanced expression of HO (p < 0.01) and collagen type III (p < 0.001) gene on postoperative day 14. Enhanced expression of NOS gene was seen only on postoperative day 4 (p < 0.01). Immunohistochemistry revealed that immunoreactivity to HO-1 had much in common in neural cells and fibers, although immunoreactivity to HO-2 and iNOS was relatively weak. This study suggested gene expression of HO, especially HO-1, was more dramatically changed than NOS, and was upregulated simultaneously with increase of collagen type III after obstruction. HO systems could be involved in the pathogenesis of bladder dysfunction related to increase of collagen type III after obstruction.
Inaba
Mitsuhiko
M
Department of Urology, Kyoto Prefectural University of Medicine, Kyoto, Japan. mi-inaba@sannet.ne.jp
Ukimura
Osamu
O
Yaoi
Takeshi
T
Kawauchi
Akihiro
A
Fushiki
Shinji
S
Miki
Tsuneharu
T
D000818:Animals; D024061:Collagen Type III / Q000502:physiology*; D005260:Female; D006419:Heme Oxygenase (Decyclizing) / Q000502:physiology*; D051547:Heme Oxygenase-1 / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation; D001748:Urinary Bladder Neck Obstruction / Q000378:metabolism*
D024061:Collagen Type III; D019001:Nitric Oxide Synthase; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; C105245:heme oxygenase-2