A direct enzyme immunoassay for the measurement of furosemide in horse plasma.
A new enzyme immunoassay (EIA) for the measurement of furosemide in horse plasma is described. The lower limit of detection of this EIA method was 7.8 ng/ml. The intra-and inter-assay coefficients of variation ranged from 2.5% to 4.9% and 7.5% to 9.8%, respectively. Cross-reactivity with other compounds was not observed. There was a high correlation (r2=0.987) between the high-performance liquid chromatography and EIA results obtained for furosemide concentrations in horse plasma. These results indicate that the newly developed EIA method is useful for the quantitative analysis of furosemide in horse plasma.
Nagata
Shun-ichi
S
Laboratory of Racing Chemistry, Japan.
Kurosawa
Masahiko
M
Kuwajima
Masao
M
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D004305:Dose-Response Relationship, Drug; D005665:Furosemide / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D006736:Horses / Q000097:blood*; D007124:Immunoenzyme Techniques / Q000379:methods* / Q000662:veterinary*; D012680:Sensitivity and Specificity
D005665:Furosemide