[Antagonistic effects of Se-rich Spirulina platensis on rat liver fibrosis].
OBJECTIVE
Antagonistic effects of supplement of Se-rich Spirulina platensis (Se-SP) on hepatocirrhosis were investigated with the rat model of liver fibrosis induced by intraperitoneal injection 3% thioacetamide (TAA).


METHODS
Parameters of routine liver function, content of malondialdehyde (MDA) and activities of glutathione peroxidase (GPx) and superoxide dismutase (SOD) in rat serum were determined by colorimetry. Content of selenium (Se) was measured by DAN fluorometry method and hyaluronic acid (HA) was detected by radio-immunoassay. Liver fibrosis was diagnosed by HE staining and relative contents of collagen (RCC) were estimated by Masson's trichrome staining.


RESULTS
Parameters of liver function in Se-SP group were most recovered in all protective groups. Compared with the model groups, contents of MDA and HA were lower, whereas activities of GPx and SOD were higher (P < 0.05) in rats serum of Se-SP group. The RCC in rats liver of Se-SP group were lower than those of the model groups, where the liver fibrosis were identified dominantly to degree I according to pathological diagnosis. Moreover, Se content in rats serum had positive correlation (r = 0.645) with activity of GPx while a negative correlation (r = 0.675) with MDA level.


CONCLUSIONS
The results indicated that Se-SP have detectable antagonistic effects to liver fibrosis, and suggested that enhancement of antioxidation level and liver reserve function might be associated with these effects.
Huang
Zhi
Z
College of Life Science and Technology, Ji'nan University,Guangzhou 510632, China. tzhwj@jnu.edu.cn
Zheng
Wenjie
W
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D005979:Glutathione Peroxidase / Q000378:metabolism; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000188:drug therapy / Q000517:prevention & control*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012643:Selenium / Q000494:pharmacology* / Q000627:therapeutic use; D053545:Spirulina / Q000737:chemistry*
D000975:Antioxidants; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D012643:Selenium