Addition of neuA, the gene encoding N-acylneuraminate cytidylyl transferase, increases the discriminatory ability of the consensus sequence-based scheme for typing Legionella pneumophila serogroup 1 strains.
The standard sequence-based method for the typing of Legionella pneumophila serogroup 1 strains was extended by using the gspA and neuA alleles. The use of neuA as a seventh allele for typing significantly increased the index of discrimination calculated for a panel of unrelated strains (from 0.932 to 0.963) and subdivided some known large common complexes (e.g., 1,4,3,1,1,1). This modification to the standard method is proposed as the method of choice in the epidemiological investigation of L. pneumophila infections.
Ratzow
Sandra
S
Institut für Medizinische Mikrobiologie und Hygiene, TU Dresden, Fiedlerstrasse 42, D-01307 Dresden, Germany.
Gaia
Valeria
V
Helbig
Jürgen Herbert
JH
Fry
Norman K
NK
Lück
Paul Christian
PC
D000483:Alleles; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D016384:Consensus Sequence; D006360:Heat-Shock Proteins / Q000235:genetics; D006801:Humans; D016952:Legionella pneumophila / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D007877:Legionnaires' Disease / Q000453:epidemiology / Q000382:microbiology; D008969:Molecular Sequence Data; D000216:N-Acylneuraminate Cytidylyltransferase / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012703:Serotyping
D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; C090745:global stress protein A, bacteria; D000216:N-Acylneuraminate Cytidylyltransferase