Polypyrrole-polyvinyl sulphonate film based disposable nucleic acid biosensor.
Double stranded calf thymus deoxyribonucleic acid entrapped polypyrrole-polyvinyl sulphonate (dsCT-DNA-PPy-PVS) films fabricated onto indium-tin-oxide (ITO) coated glass plates have been used to detect organophosphates such as chlorpyrifos and malathion. These disposable dsCT-DNA-PPy-PVS/ITO bioelectrodes have been characterized using cyclic voltammetry, Fourier-transform-infra-red (FTIR) spectroscopy and atomic force microscopy (AFM), respectively. These biosensing electrodes have a response time of 30s, are stable for about 5 months when stored in desiccated conditions at 25 degrees C and can be used to amperometrically detect chlorpyrifos (0.0016-0.025 ppm) and malathion (0.17-5.0), respectively. The additive effect of these pesticides on the amperometric response of the disposable dsCT-DNA-PPy-PVS/ITO bioelectrodes has also been investigated.
Prabhakar
Nirmal
N
Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, New Delhi, India.
Arora
Kavita
K
Singh
Surinder P
SP
Pandey
Manoj K
MK
Singh
Harpal
H
Malhotra
Bansi D
BD
D015374:Biosensing Techniques; D004390:Chlorpyrifos / Q000032:analysis; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D008294:Malathion / Q000032:analysis; D018625:Microscopy, Atomic Force; D011108:Polymers / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
D011108:Polymers; D011145:Polyvinyls; D011758:Pyrroles; C019050:polyvinyl sulfate; C067635:polypyrrole; D004247:DNA; D004390:Chlorpyrifos; D008294:Malathion