title: The use of the elevated plus maze as an assay of anxiety-related behavior in rodents.
abstract: The elevated plus maze is a widely used behavioral assay for rodents and it has been validated to assess the anti-anxiety effects of pharmacological agents and steroid hormones, and to define brain regions and mechanisms underlying anxiety-related behavior. Briefly, rats or mice are placed at the junction of the four arms of the maze, facing an open arm, and entries/duration in each arm are recorded by a video-tracking system and observer simultaneously for 5 min. Other ethological parameters (i.e., rears, head dips and stretched-attend postures) can also be observed. An increase in open arm activity (duration and/or entries) reflects anti-anxiety behavior. In our laboratory, rats or mice are exposed to the plus maze on one occasion; thus, results can be obtained in 5 min per rodent.
authors.affiliation: Department of Psychology, The University at Albany-SUNY, Albany, New York, USA. aawalf@yahoo.com
authors.forename: Alicia A
authors.initials: AA
authors.lastname: Walf
authors.forename: Cheryl A
authors.initials: CA
authors.lastname: Frye
mesh_terms: D000818:Animals; D001007:Anxiety; D003625:Data Collection / Q000379:methods*; D005106:Exploratory Behavior / Q000502:physiology*; D051379:Mice; D019370:Observation; D051381:Rats