title: A simple method to determine concentration of enantiomers in enzyme-catalyzed kinetic resolution.
abstract: Kinetic resolutions play important roles in industrial biotransformations for production of optical pure compounds from racemic substrates. A simple method, based on enantiomeric excess of both substrate (ee (S)) and the corresponding product (ee (P)), was developed for determination of concentration of enantiomers in kinetic resolution. Since only relative quantity (ee) was required in the proposed method, calibration and cumbersome quantitative sample handling can be avoided and analytical accuracy can be greatly improved.
authors.affiliation: Institute of Bioengineering, Zhejiang University of Technology, Hangzhou, PR China.
authors.forename: Ren-Chao
authors.initials: RC
authors.lastname: Zheng
authors.forename: Yu-Guo
authors.initials: YG
authors.lastname: Zheng
authors.forename: Yin-Chu
authors.initials: YC
authors.lastname: Shen
mesh_terms: D000581:Amidohydrolases / Q000378:metabolism*; D002384:Catalysis; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D007700:Kinetics; D013237:Stereoisomerism; D013379:Substrate Specificity
chemical_list: D000581:Amidohydrolases; C019665:amidase