title: Dendrimer-like DNA-based fluorescence nanobarcodes.
abstract: A major challenge in clinical diagnostics and environmental analysis is the difficulty in rapid and sensitive detection of multiple target molecules simultaneously (i.e., multiplexed detections). Our group has designed and synthesized a dendrimer-like DNA (DL-DNA) that is multivalent and anisotropic; using this unique DNA structure, we have developed a fluorescence-tagged nanobarcode system for multiplex detection. This nanobarcode system allows the rapid and sensitive detection of multiple pathogens simultaneously using the ratios of two different fluorescent dyes, green and red, with which different DL-DNAs are labeled. The key step of our nanobarcode model lies in the monodisperse preparation of DL-DNA. Two methods, solution phase and solid phase, are presented here. With slight modifications, this platform technology can also be extended to the multiplexed detection of RNA and proteins. This protocol can be completed in 2-5 d.
authors.affiliation: Department of Biological and Environmental Engineering, Cornell University, Ithaca, NY 14853-5701, USA.
authors.forename: Soong Ho
authors.initials: SH
authors.lastname: Um
authors.forename: Jong Bum
authors.initials: JB
authors.lastname: Lee
authors.forename: Sang Yeon
authors.initials: SY
authors.lastname: Kwon
authors.forename: Yougen
authors.initials: Y
authors.lastname: Li
authors.forename: Dan
authors.initials: D
authors.lastname: Luo
mesh_terms: D004247:DNA / Q000737:chemistry*; D050091:Dendrimers / Q000737:chemistry*; D001330:Electronic Data Processing / Q000379:methods*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization / Q000379:methods
chemical_list: D050091:Dendrimers; D005456:Fluorescent Dyes; D004247:DNA