Isolation and characterization of a spore germination inhibitor from Streptomyces sp. CB-1-1, a phytopathogen causing root tumor of melon.
The germination rate and activation conditions of spores were examined for four strains of Streptomyces sp., a phytopathogen causing root tumor of melon. An inhibitor was isolated from the agar-cultured material of strain CB-1-1 and then characterized. The inhibitor selectively acted on spore germination and did not affect hyphal growth, and inhibition was abolished by washing the spores in water. The inhibitor was produced by an agar culture, and most of the inhibitor existed in the spores. The IC(50) value for the inhibitor was approximately 0.25 microg/ml.
Aoki
Yuu
Y
Science of Plant and Animal Production Course, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Yoshida
Masahiro
M
Kawaide
Hiroshi
H
Abe
Hiroshi
H
Natsume
Masahiro
M
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D019662:Cucurbitaceae / Q000382:microbiology; D003470:Culture Media; D006358:Hot Temperature; D025301:Hyphae / Q000187:drug effects / Q000254:growth & development; D009682:Magnetic Resonance Spectroscopy; D010935:Plant Diseases / Q000382:microbiology*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet; D013172:Spores, Fungal / Q000187:drug effects* / Q000254:growth & development; D013302:Streptomyces / Q000254:growth & development / Q000378:metabolism*; D013501:Surface-Active Agents / Q000494:pharmacology
D003470:Culture Media; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate