Effects of octamethylcyclotetrasiloxane (D4) on the luteinizing hormone (LH) surge and levels of various reproductive hormones in female Sprague-Dawley rats.
The objectives of this study were to assess the potential for D(4) to suppress the pre-ovulatory lutenizing hormone (LH) surge, to block or delay ovulation, and to evaluate potential effects on reproductive hormones in rats. Female Sprague-Dawley Crl:CD (SD) IGS BR rats received whole-body vapor inhalation exposure to D(4) (0, 700, or 900ppm) 6h per day for 3 days. Trunk blood obtained on proestrus at 10a.m. was evaluated for levels of follicle stimulating hormone (FSH), estradiol (E2), estrone (E1), and progesterone (P4). Other rats had serial blood samples collected via cannula at 2, 4, 6, 8, and 10p.m. on the day of proestrus and plasma evaluated for LH and prolactin (PRL). Trunk blood was collected at 8a.m. of estrus and plasma evaluated for FSH, E2, E1, and P4. At 10a.m. on proestrus, significant increases in E1 levels in the 700 and 900ppm groups and significant increases in P4 levels in the 900ppm group were noted. At 8a.m. on estrus, significant increases in E1, E2, in the E1/E2 ratio and decreases in FSH were noted in the 700 and 900ppm groups. The major effect on the LH profile was observed most clearly when the rats were grouped by ovulatory status, animals that did or did not ovulate. Regardless of treatment, suppression of the LH surge correlated with blocked ovulation. The percentage of rats that ovulated was (700ppm, 42%; 900ppm, 31%) compared to controls (79%). Overall, the data indicate that high exposures to D(4) attenuated the pre-ovulatory LH surge and significantly decreased the portion of female rats that ovulated.
Quinn
Anne L
AL
Dow Corning Corporation, Midland, MI 48686-0994, United States. Anne.Quinn@dowcorning.com
Dalu
Abe
A
Meeker
Linda S
LS
Jean
Paul A
PA
Meeks
Robert G
RG
Crissman
James W
JW
Gallavan
Robert H
RH
Plotzke
Kathleen P
KP
D000393:Air Pollutants / Q000737:chemistry / Q000633:toxicity*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001921:Brain / Q000187:drug effects / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D052244:Endocrine Disruptors / Q000737:chemistry / Q000633:toxicity*; D004958:Estradiol / Q000097:blood; D004970:Estrone / Q000097:blood; D030762:Estrous Cycle / Q000097:blood / Q000187:drug effects*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D019570:Inhalation Exposure; D007986:Luteinizing Hormone / Q000097:blood*; D009929:Organ Size / Q000187:drug effects; D010053:Ovary / Q000187:drug effects / Q000473:pathology; D010060:Ovulation / Q000097:blood / Q000187:drug effects*; D010063:Ovum / Q000187:drug effects / Q000473:pathology; D011374:Progesterone / Q000097:blood; D011388:Prolactin / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D012833:Siloxanes / Q000737:chemistry / Q000633:toxicity*; D013997:Time Factors; D014599:Uterus / Q000187:drug effects / Q000473:pathology; D014835:Volatilization
D000393:Air Pollutants; D052244:Endocrine Disruptors; D012833:Siloxanes; D004970:Estrone; D011374:Progesterone; D004958:Estradiol; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; C024064:octamethylcyclotetrasiloxane