title: Interaction of dipalmitoyl phosphatidylserine with ethanol: induction of an ordered gel phase at room temperature.
abstract: Using differential scanning calorimetry and small and wide-angle X-ray diffraction, we show that, unlike the saturated phosphatidylcholines, for which ethanol induces chain interdigitation in the gel state, and unlike natural phosphatidylserine in which the gel state is almost unaffected by the addition of ethanol, dipalmitoyl phosphatidylserine (DPPS) assumes an ordered structure after incubation at room temperature in the presence of as little as 5% (v/v) ethanol. In the liquid crystalline state, a progressive decrease in the interbilayer spacing is observed as a function of ethanol concentration, similar to what is found for natural phosphatidylserine (PS) and 1-palmitoyl-2-oleoyl-phosphatidylserine (POPS). The 0.37 molar fraction of cholesterol in the DPPS dispersion in the presence of 10% (v/v) ethanol, does not prevent the formation of the ordered gel.
authors.affiliation: Chemical Research Infrastructure Unit, Faculty of Chemistry, The Weizmann Institute of Science, 76100 Rehovot, Israel. ellen.wachtel@weizmann.ac.il
authors.forename: E
authors.initials: E
authors.lastname: Wachtel
authors.forename: D
authors.initials: D
authors.lastname: Bach
authors.forename: I R
authors.initials: IR
authors.lastname: Miller
authors.forename: N
authors.initials: N
authors.lastname: Borochov
mesh_terms: D002152:Calorimetry, Differential Scanning; D000431:Ethanol / Q000737:chemistry*; D010718:Phosphatidylserines / Q000737:chemistry*; D013696:Temperature; D014961:X-Ray Diffraction
chemical_list: D010718:Phosphatidylserines; C038694:dipalmitoylphosphatidylserine; D000431:Ethanol