A novel method for evaluation of ethanol oxidation in living brain.
We propose a method for intravital examination of ethanol metabolism in rat brain. Calipsol-anesthetized rats were fixed in stereotaxis and ethanol solution was infused into the lateral cerebral ventricle via an orifice in the skull at a constant rate with a syringe or micro-pump. Elimination of ethanol and production of acetaldehyde (ethanol metabolite) were measured in perfusate samples from the cisterna magna by gas chromatography. The method is highly sensitive, reliable, and reproducible and allows to study the kinetics and enzyme mechanisms of ethanol oxidation in the brain and regulation of this process.
Zimatkin
S M
SM
Grodnenskii State Medical University, Grodno, Belarus'. zimatkin@grsmu.by
Buben
A L
AL
D000079:Acetaldehyde / Q000032:analysis / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002849:Chromatography, Gas; D000431:Ethanol / Q000008:administration & dosage / Q000032:analysis / Q000378:metabolism*; D005069:Evaluation Studies as Topic; D007276:Injections, Intraventricular; D007700:Kinetics; D010084:Oxidation-Reduction; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000431:Ethanol; D000079:Acetaldehyde