An in vitro study of the toxic effects of Stachybotrys chartarum metabolites on lung cells.
During a study of indoor fungal colonisation, several isolates of Stachybotrys chartarum were recovered, and the effects of metabolites from four isolates on lung epithelial Type II cells and alveolar macrophages were studied in vitro. All the isolates showed toxic effects on both cell types, and they differed only in the extent of the changes induced. In Type II cells, the number of alkaline phosphatase positive cells was reduced, the pattern of Maclura pomifera agglutinin (MPA) binding was changed, and acid phosphatase activity in alveolar macrophages was diminished. In both cell types, the production of monocyte chemotactic protein-1 (MCP-1) and tumour necrosis factor-alpha (TNF-alpha) was changed, and parameters related to antioxidant status (superoxide dismutase, glutathione peroxidase, glutathione) were decreased.
Kováciková
Zuzana
Z
Research Base of Slovak Medical University, Bratislava, Slovakia. zuzana.kovacikova@szu.sk
Tátrai
Erzsébet
E
Piecková
Elena
E
Tulinská
Jana
J
Pivovarová
Zuzana
Z
Matausic-Pisl
Mirjana
M
Kuricová
Miroslava
M
Wsolová
Ladislava
L
D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D016676:Macrophages, Alveolar / Q000187:drug effects* / Q000378:metabolism; D009183:Mycotoxins / Q000302:isolation & purification / Q000633:toxicity*; D037121:Plant Lectins / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000187:drug effects* / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D013191:Stachybotrys / Q000737:chemistry*
D009183:Mycotoxins; D037121:Plant Lectins; C034551:maclurin; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase