title: Altered expression of plant lysyl tRNA synthetase promotes tRNA misacylation and translational recoding of lysine.
abstract: The Arabidopsis thaliana lysyl tRNA synthetase (AtKRS) structurally and functionally resembles the well-characterized prokaryotic class IIb KRS, including the propensity to aminoacylate tRNA(Lys) with suboptimal identity elements, as well as non-cognate tRNAs. Transient expression of AtKRS in carrot cells promotes aminoacylation of such tRNAs in vivo and translational recoding of lysine at nonsense codons. Stable expression of AtKRS in Zea mays causes translational recoding of lysine into zeins, significantly enriching the lysine content of grain.
authors.affiliation: Department of Biochemistry, 117 Schweitzer Hall, University of Missouri-Columbia, Columbia, MO 65211, USA.
authors.forename: Xing Rong
authors.initials: XR
authors.lastname: Wu
authors.forename: Alexander
authors.initials: A
authors.lastname: Kenzior
authors.forename: David
authors.initials: D
authors.lastname: Willmot
authors.forename: Sarah
authors.initials: S
authors.lastname: Scanlon
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Chen
authors.forename: Andrey
authors.initials: A
authors.lastname: Topin
authors.forename: Shao Hua
authors.initials: SH
authors.lastname: He
authors.forename: Ashley
authors.initials: A
authors.lastname: Acevedo
authors.forename: William R
authors.initials: WR
authors.lastname: Folk
mesh_terms: D000215:Acylation; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology*; D001483:Base Sequence; D015139:Blotting, Southern; D017931:DNA Primers; D008239:Lysine / Q000235:genetics*; D008250:Lysine-tRNA Ligase / Q000235:genetics*; D008969:Molecular Sequence Data; D012357:RNA, Transfer, Lys / Q000235:genetics / Q000378:metabolism*; D003313:Zea mays / Q000201:enzymology
chemical_list: D017931:DNA Primers; D012357:RNA, Transfer, Lys; D008250:Lysine-tRNA Ligase; D008239:Lysine