title: Dengue viruses can infect human primary lung epithelia as well as lung carcinoma cells, and can also induce the secretion of IL-6 and RANTES.
abstract: Dengue viruses (DENV) are herein demonstrated for the first time as being able to infect and replicate in human primary lung epithelium and various lung cancer cell lines. The detection of dengue virus particles and viral negative strand RNA synthesis in the cell, in conjunction with the release of viral progenies in culture supernatants, support the notion that lung cells are susceptible to dengue virus infection. The replication efficiency of DENV in lung cancer cells from high to low is: DEN-2 (dengue virus type-2), DEN-3, DEN-4 and DEN-1. Moreover, the susceptibility of the six lung cancer cell lines to DEN-2 infection is: SW1573>A549>H1435; H23; H520; Bes2B. DEN-2 infection significantly increased the expression levels of IL-6 and RANTES in four of the six lung cancer cell lines, which is consistent with the high expression levels of these molecules in DHF/DSS patients. IL-6 expression induced by DEN-2 infection was NF-kappaB dependent. In summary, our results indicate that lung epithelial cell is a possible target of dengue viruses and IL-6 and RANTES may play pivotal roles in lung related immuno-pathogenesis.
authors.affiliation: Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan, ROC.
authors.forename: Ying-Ray
authors.initials: YR
authors.lastname: Lee
authors.forename: Ching-Yao
authors.initials: CY
authors.lastname: Su
authors.forename: Nan-Haw
authors.initials: NH
authors.lastname: Chow
authors.forename: Wu-Wei
authors.initials: WW
authors.lastname: Lai
authors.forename: Huan-Yao
authors.initials: HY
authors.lastname: Lei
authors.forename: Chia-Lun
authors.initials: CL
authors.lastname: Chang
authors.forename: Tsuey-Yu
authors.initials: TY
authors.lastname: Chang
authors.forename: Shun-Hua
authors.initials: SH
authors.lastname: Chen
authors.forename: Yee-Shin
authors.initials: YS
authors.lastname: Lin
authors.forename: Trai-Ming
authors.initials: TM
authors.lastname: Yeh
authors.forename: Hsiao-Sheng
authors.initials: HS
authors.lastname: Liu
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D018946:Chemokine CCL5 / Q000096:biosynthesis*; D003716:Dengue Virus / Q000145:classification / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D004847:Epithelial Cells / Q000276:immunology / Q000821:virology; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000235:genetics; D008168:Lung / Q000166:cytology / Q000276:immunology / Q000821:virology; D008175:Lung Neoplasms / Q000276:immunology / Q000821:virology; D016328:NF-kappa B / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012367:RNA, Viral / Q000096:biosynthesis / Q000235:genetics; D014779:Virus Replication
chemical_list: D018946:Chemokine CCL5; C508600:IL6 protein, human; D015850:Interleukin-6; D016328:NF-kappa B; D012367:RNA, Viral