Differentiation of Bacillus anthracis, B. cereus, and B. thuringiensis by using pulsed-field gel electrophoresis.
A pulsed-field gel electrophoresis (PFGE) method was developed for discriminating Bacillus anthracis from B. cereus and B. thuringiensis. A worldwide collection of 25 B. anthracis isolates showed high-profile homology, and these isolates were unambiguously distinguished from B. cereus and B. thuringiensis isolates by cluster analysis of the whole-genome macrorestriction enzyme digestion patterns generated by NotI.
Zhong
Wenwan
W
Bioscience Division, MS M888, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
Shou
Yulin
Y
Yoshida
Thomas M
TM
Marrone
Babetta L
BL
D001408:Bacillus anthracis / Q000145:classification* / Q000235:genetics; D001409:Bacillus cereus / Q000145:classification* / Q000235:genetics; D001413:Bacillus thuringiensis / Q000145:classification* / Q000235:genetics; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000032:analysis* / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D010802:Phylogeny
D004269:DNA, Bacterial; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases