Inhibitory effect of obovatal on the migration and invasion of HT1080 cells via the inhibition of MMP-2.
Because the activation of matrix metalloproteinases (MMP) is a key factor in the metastatic process, compounds with the ability to inhibit MMP activity have a potential in the treatment of tumor. From the examination of 2000 plant extracts, obovatal isolated from the extract of the leaves of Magnolia obovata THUNB was a potent inhibitor of MMP-2 enzyme in vitro. In human fibrosarcoma cells (HT1080) activated with MMP-2, obovatal inhibited MMP-2 enzyme activity and expression. In addition, the compound blocked migration and invasion of the cells. This study demonstrates that obovatal exerts its anticancer effects through blocking migration and invasion by inhibition of MMP-2 expression and activity, and also will be a good lead molecule for the development of anti-tumor drug.
Lee
Su-Kyung
SK
Korea Research Institute of Bioscience and Biotechnology, 52 Uendong, Yusung, Taejon 305-600, Republic of Korea.
Chun
Hyo Kon
HK
Yang
Jae Young
JY
Han
Dong Cho
DC
Son
Kwang-Hee
KH
Kwon
Byoung-Mog
BM
D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D006801:Humans; D031566:Magnolia / Q000737:chemistry; D061965:Matrix Metalloproteinase Inhibitors; D009361:Neoplasm Invasiveness; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D011480:Protease Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*
D061965:Matrix Metalloproteinase Inhibitors; D010936:Plant Extracts; D011480:Protease Inhibitors