title: Regulation of cytokine synthesis in cardiac surgery: Role of extracorporeal circuit and humoral mediators in vivo and in vitro.
abstract: OBJECTIVE
  Cardiopulmonary bypass (CPB) impairs monocyte and neutrophil proliferation, cytokine synthesis, and antigen presentation. This study compares in vivo data with results from an extracorporeal circulation (ECC) model, distinguishing direct effects on cytokine synthesis from regulatory mechanisms.
  
  
  METHODS
  Whole blood from 18 patients prior to, during and after CPB was stimulated with lipopolysaccharide (LPS). Tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, and IL-8 levels were measured. Additionally, blood from 4 volunteers was circulated in an ECC model. Cytokine levels were measured before and during mock ECC.
  
  
  RESULTS
  LPS-induced cytokine synthesis was reduced after CPB (TNF-alpha: 11 %; IL-6: 29 %; IL-8: 48 % of preoperative values, all p < 0.001). In mock ECC, cytokine production (except IL-8) was suppressed: TNF-alpha production was lowest 60 min after starting ECC, IL-6 synthesis was lowest at 90 min (33 % and 15 % vs. pre-ECC levels; both p < 0.001). Patient sera contained cytokine-inhibitory activity after CPB, an activity not found in mock ECC.
  
  
  CONCLUSIONS
  (1) In patients, CPB induces early transient LPS hyporesponsiveness; (2) blood contact with foreign surfaces induces LPS hyporesponsiveness; (3) serum cytokine-inhibitory activities are released after CPB, but not in mock ECC. Impaired leukocyte function may explain increased susceptibility to infections after CPB.
authors.affiliation: Martin-Luther-University Halle-Wittenberg, Department of Cardiac- and Thoracic Surgery, Ernst-Grube-Str. 40, 06120, Halle, Germany. Jochen.Boergemann@t-online.de
authors.forename: J
authors.initials: J
authors.lastname: Börgermann
authors.forename: S
authors.initials: S
authors.lastname: Flohé
authors.forename: R J
authors.initials: RJ
authors.lastname: Scheubel
authors.forename: O
authors.initials: O
authors.lastname: Kuss
authors.forename: A
authors.initials: A
authors.lastname: Simm
authors.forename: F U
authors.initials: FU
authors.lastname: Schade
authors.forename: I
authors.initials: I
authors.lastname: Friedrich
mesh_terms: D000368:Aged; D001772:Blood Cell Count; D006348:Cardiac Surgical Procedures; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis* / Q000097:blood; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects; D008875:Middle Aged
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides