title: [In vitro cultivation of fields isolates of Plasmodium falciparum in Mali].
abstract: Malaria immunology, molecular biology and pathogenicity studies often require the adaptation of Plasmodium falciparum field isolates to continuous in vitro cultivation. For this purpose we have established propagation protocols of asexual erythrocytic stages of P. falciparum samples from malaria patients or asymptomatic carriers in Mali. The parasites were grown in standard culture medium supplemented by human serum and in a culture medium without human serum but supplemented by AlbuMax 1. The candle jar environment and tissue culture flasks gassed with 5% CO2, 5% O2 and 90% N2 obtained from a portable gas mixer were used. Protocols for parasite cultivation in a resource-poor setting were developed. These protocols were successfully applied to fresh isolates in Mali as well as to blood samples frozen in liquid nitrogen and shipped to a laboratory in U.S.A.
authors.affiliation: Centre de recherche et de formation sur le paludisme, Département d'épidemiologie des affections parasitaires, Faculté de medecine, de pharmacie et d'odonto-stomatologie, Université de Bamako, Mali. adjimde@mrtcbko.org
authors.forename: A A
authors.initials: AA
authors.lastname: Djimde
authors.forename: L
authors.initials: L
authors.lastname: Kirkman
authors.forename: L
authors.initials: L
authors.lastname: Kassambara
authors.forename: M
authors.initials: M
authors.lastname: Diallo
authors.forename: C V
authors.initials: CV
authors.lastname: Plowe
authors.forename: T E
authors.initials: TE
authors.lastname: Wellems
authors.forename: O K
authors.initials: OK
authors.lastname: Doumbo
mesh_terms: D000818:Animals; D002245:Carbon Dioxide / Q000494:pharmacology; D015925:Cryopreservation; D003470:Culture Media; D016172:DNA Fingerprinting; D016054:DNA, Protozoan / Q000235:genetics; D004912:Erythrocytes / Q000469:parasitology; D006801:Humans; D016778:Malaria, Falciparum / Q000469:parasitology; D008302:Mali; D010274:Parasitology / Q000295:instrumentation / Q000379:methods*; D010963:Plasmodium falciparum / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification
chemical_list: D003470:Culture Media; D016054:DNA, Protozoan; D002245:Carbon Dioxide