Optimum conditions for culturing of human bone marrow and adipose tissue mesenchymal precursor cells.
We present a technology of culturing of human mesenchymal stem cells under conditions excluding the presence of animal sera or additional growth factors, but preserving high proliferative potential and the capacity of these cells to multilineage differentiation. Human umbilical serum was used as the alternative material. We found that in the presence of human umbilical serum mesenchymal stem cells more effectively proliferate and retain their differentiation capacity. The proposed technology yields 109-1010 morphologically and functionally identical cells.
Romanov
Yu A
YA
Research and Practical Laboratory of Human Stem Cells , Russian Cardiology Research and Production Complex, Federal Agency of Health Care and Social Development. romanov@cardio.ru
Darevskaya
A N
AN
Kabaeva
N V
NV
Antonova
O A
OA
D000273:Adipose Tissue / Q000166:cytology*; D015415:Biomarkers / Q000032:analysis; D001854:Bone Marrow Cells / Q000166:cytology*; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002455:Cell Division; D003470:Culture Media; D005312:Fetal Blood / Q000166:cytology; D006801:Humans; D007150:Immunohistochemistry; D007700:Kinetics; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009474:Neurons / Q000166:cytology; D010641:Phenotype
D015415:Biomarkers; D003470:Culture Media