title: Use of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide for rapid detection of methicillin-resistant Staphylococcus aureus by resonance light scattering.
abstract: A rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) using resonance light scattering (RLS) on an ordinary fluorescence spectrometer was developed. The viable MRSA reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) to produce insoluble particles which displayed intense resonance scattering light. It showed a linear relationship between the number of viable MRSA and the RLS intensity. Dead MRSA were unable to reduce MTT. MRSA exposed to flavonoids extracted from Marchantia convoluta (MCF) showed a MCF concentration-dependent inhibition of the ability to reduce MTT. The RLS could, in combination with the MTT assay, be a rapid and sensitive detection method for vitro-cultured MRSA.
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha, PR China.
authors.forename: Jian Bo
authors.initials: JB
authors.lastname: Xiao
authors.forename: Jing Wen
authors.initials: JW
authors.lastname: Chen
authors.forename: Feng Lian
authors.initials: FL
authors.lastname: Ren
authors.forename: Chun Sheng
authors.initials: CS
authors.lastname: Yang
authors.forename: Ming
authors.initials: M
authors.lastname: Xu
mesh_terms: D015169:Colony Count, Microbial; D005419:Flavonoids / Q000302:isolation & purification; D008027:Light; D016106:Methicillin Resistance; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013211:Staphylococcus aureus / Q000302:isolation & purification*; D013778:Tetrazolium Salts / Q000737:chemistry*; D013844:Thiazoles / Q000737:chemistry*
chemical_list: D005419:Flavonoids; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue