Microbial life in Champagne Pool, a geothermal spring in Waiotapu, New Zealand.
Surveys of Champagne Pool, one of New Zealand's largest terrestrial hot springs and rich in arsenic ions and compounds, have been restricted to geological and geochemical descriptions, and a few microbiological studies applying culture-independent methods. In the current investigation, a combination of culture and culture-independent approaches were chosen to determine microbial density and diversity in Champagne Pool. Recovered total DNA and adenosine 5'-triphosphate (ATP) content of spring water revealed relatively low values compared to other geothermal springs within New Zealand and are in good agreement with low cell numbers of 5.6 +/- 0.5 x 10(6) cells/ml obtained for Champagne Pool water samples by 4',6-diamidino-2-phenylindole (DAPI) staining. Denaturing Gradient Gel Electrophoresis (DGGE) and 16S rRNA (small-subunit ribosomal nucleic acid) gene clone library analyses of environmental DNA indicated the abundance of Sulfurihydrogenibium, Sulfolobus, and Thermofilum-like populations in Champagne Pool. From these results, media were selected to target the enrichment of hydrogen-oxidizing and sulfur-dependent microorganisms. Three isolates were successfully obtained having 16S rRNA gene sequences with similarities of approximately 98% to Thermoanaerobacter tengcongensis, 94% to Sulfurihydrogenibium azorense, and 99% to Thermococcus waiotapuensis, respectively.
Hetzer
Adrian
A
Thermophile Research Unit, University of Waikato, Te Whare Wananga o Waikato, Gate 1 Knighton Road, Private Bag 3105, Hamilton, 3240, New Zealand. hetzer.adrian@web.de
Morgan
Hugh W
HW
McDonald
Ian R
IR
Daughney
Christopher J
CJ
D000255:Adenosine Triphosphate / Q000032:analysis; D001105:Archaea / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification*; D001419:Bacteria / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification*; D044822:Biodiversity; D003470:Culture Media; D019641:DNA, Archaeal / Q000032:analysis; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D015723:Gene Library; D019015:Geologic Sediments / Q000737:chemistry / Q000382:microbiology*; D045482:Hot Springs / Q000737:chemistry / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009520:New Zealand; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D021521:Ribotyping; D016944:Sulfolobus / Q000302:isolation & purification; D013696:Temperature; D046968:Thermoanaerobacter / Q000302:isolation & purification; D019712:Thermococcus / Q000302:isolation & purification; D041561:Thermofilaceae / Q000302:isolation & purification; D014871:Water Microbiology
D003470:Culture Media; D019641:DNA, Archaeal; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D000255:Adenosine Triphosphate