[Effect of grape procyanidins on the protein kinase and proliferative cell nuclear antigen protein expression of mice hepatic cells].
OBJECTIVE
To study the effect of grape procyanidins (GPC) on the protein kinase C (PKC) and proliferative cell nuclear antigen (PCNA) expression in mice hepatic cells.


METHODS
The mice were orally given at the doses of GPC and killed after 30 days. The proliferation activity of the hepatic cells was determined by MYT assay, and the levels of PKC and PCNA protein expression in the cell were measured by immunohistochemistry method.


RESULTS
The expression rates of PKC and PCNA protein of the high-dose GPC group were 47.62% and 82.76% respectively,and those of the negative control group were 6.42% and 32.62%. The differences between the two groups were significant (P < 0.05). The proliferation of mice hepatic cells increased with the dose of GPC in different groups.


CONCLUSIONS
GPC could promote the PKC protein expression and improve the proliferative activity of the mice hepatic cells.
Liu
Hui
H
Medical College of Qingdao University, Qingdao 266021, China.
Zhong
Jinyi
J
Yu
Ping
P
Li
Lei
L
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D044946:Biflavonoids / Q000494:pharmacology*; D002392:Catechin / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D044945:Proanthocyanidins / Q000494:pharmacology*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D011897:Random Allocation; D027843:Vitis / Q000737:chemistry
D000975:Antioxidants; D044946:Biflavonoids; D044945:Proanthocyanidins; D018809:Proliferating Cell Nuclear Antigen; C017674:procyanidin; D002392:Catechin; D011493:Protein Kinase C