title: Ontogenesis of haemocytes in shrimp (Fenneropenaeus chinensis) studied with probes of monoclonal antibody.
abstract: Monoclonal antibodies (Mabs) specifically against haemocytes of shrimp (Fenneropenaeus chinensis) were employed to study the ontogenesis of haemocytes by immunofluorescence assay technique (IFAT) with the aids of avidin-biotin complex method. Different developmental stages were identified by means of histochemical staining. Stages including blastula, gastrula, limb bud stage, embryonic-nauplius and nauplius were examined. With Mab 2A3 against both hyline, semi-granular and granular haemocytes, the first positive cells were detected at the limb bud stage of the embryos. Distinguishable positive haemocytes with a diameter of about 7 microm appeared in embryonic-nauplius. Whereas, with Mab 1H11 specific to cytoplasmic granulars in haemocytes, the first positive granular haemocytes were observed at the stage of embryonic-nauplius. Increased number of both total and granular haemocytes at the stage of nauplius impressed a possible enhancement of the immune system as the embryogenesis progressed.
authors.affiliation: Laboratory of Pathology and Immunology of Aquatic Animals, LMMEC, Ocean University of China, No. 5, Yushan Road, Qingdao 266003, PR China.
authors.forename: Yingbo
authors.initials: Y
authors.lastname: Lin
authors.forename: Wenbin
authors.initials: W
authors.lastname: Zhan
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.forename: Zhidong
authors.initials: Z
authors.lastname: Zhang
authors.forename: Xiumei
authors.initials: X
authors.lastname: Wei
authors.forename: Xiuzhen
authors.initials: X
authors.lastname: Sheng
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D003429:Cross Reactions; D003594:Cytoplasmic Granules / Q000276:immunology / Q000648:ultrastructure; D047108:Embryonic Development; D006434:Hemocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D007113:Immunity, Innate; D033561:Penaeidae / Q000166:cytology / Q000196:embryology / Q000254:growth & development / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal