title: Cardioprotective action of urocortin in early pre- and postconditioning.
abstract: Pre- and postconditioning are powerful endogenous adaptive phenomenon of the organism whereby different stimuli enhance the tolerance against various types of stress. Urocortin (Ucn), member of the corticotropin-releasing factor (CRF) family has potent effects on the cardiovascular system. The aim of this article was to investigate the action of Ucn on cultured cardiomyocytes in the process of pre- and postconditioning. Isolated neonatal rat ventricular myocytes were preconditioned with adenosine, simulated ischemia, and Ucn (10-min treatment followed by 10-min reperfusion/recovery). For detecting the effect of alternative types of preconditioning, necrosis enzyme (lactate dehydrogenase [LDH]) release, vital staining (trypan blue), and ratio of apoptosis/necrosis were examined after cardiac cells were exposed to 3-h sustained ischemia and 2-h reperfusion. Same parameters were measured in the postconditioned groups (30- or 60-min ischemia followed by postconditioning with 10-min ischemic stimulus or Ucn and 2-h reperfusion). Cells exposed to 3-h ischemia followed by 2-h reperfusion were shown as control. Our results show that LDH release a number of trypan blue-stained dead cells and the ratio of apoptotized and necrotized cells was decreased in all preconditioned groups compared with control group. In postconditioned groups LDH content of culture medium, trypan blue-positive cardiomyocytes, and the rate of apoptotic/necrotic cells was reduced contrasted with non-postconditioned group. We can conclude that preconditioning with Ucn induced such a powerful cell protective effect as adenosine and ischemia. Furthermore, postconditioning with Ucn after 60-min ischemia was more cardioprotective than ischemic postconditioning.
authors.affiliation: Department of Surgical Research and Techniques, University of Pécs, Medical Faculty, Kodaly Zoltan St. 20, Pécs, H-7624 Hungary. barbara.cserepes@aok.pte.hu
authors.forename: Barbara
authors.initials: B
authors.lastname: Cserepes
authors.forename: Gábor
authors.initials: G
authors.lastname: Jancsó
authors.forename: Balázs
authors.initials: B
authors.lastname: Gasz
authors.forename: Boglárka
authors.initials: B
authors.lastname: Rácz
authors.forename: Andrea
authors.initials: A
authors.lastname: Ferenc
authors.forename: László
authors.initials: L
authors.lastname: Benkó
authors.forename: Balázs
authors.initials: B
authors.lastname: Borsiczky
authors.forename: Mária
authors.initials: M
authors.lastname: Kürthy
authors.forename: Sandor
authors.initials: S
authors.lastname: Ferencz
authors.forename: János
authors.initials: J
authors.lastname: Lantos
authors.forename: János
authors.initials: J
authors.lastname: Gál
authors.forename: Endre
authors.initials: E
authors.lastname: Arató
authors.forename: Attila
authors.initials: A
authors.lastname: Miseta
authors.forename: György
authors.initials: G
authors.lastname: Wéber
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Róth
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002316:Cardiotonic Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D003346:Corticotropin-Releasing Hormone / Q000502:physiology*; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D019157:Ischemic Preconditioning, Myocardial; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D010865:Pilot Projects; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*; D054832:Urocortins
chemical_list: D002316:Cardiotonic Agents; D054832:Urocortins; D003346:Corticotropin-Releasing Hormone