title: Role of myofibril-inducing RNA in cardiac TnT expression in developing Mexican axolotl.
abstract: The Mexican axolotl, Ambystoma mexicanum, has been a useful animal model to study heart development and cardiac myofibrillogenesis. A naturally-occurring recessive mutant, gene "c", for cardiac non-function in the Mexican axolotl causes a failure of myofibrillogenesis due to a lack of tropomyosin expression in homozygous mutant (c/c) embryonic hearts. Myofibril-inducing RNA (MIR) rescues mutant hearts in vitro by promoting tropomyosin expression and myofibril formation thereafter. We have studied the effect of MIR on the expression of various isoforms of cardiac troponin T (cTnT), a component of the thin filament that binds with tropomyosin. Four alternatively spliced cTnT isoforms have been characterized from developing axolotl heart. The expression of various cTnT isoforms in normal, mutant, and mutant hearts corrected with MIR, is evaluated by real-time RT-PCR using isoform specific primer pairs; MIR affects the total transcription as well as the splicing of the cTnT in axolotl heart.
authors.affiliation: Department of Biomedical Science, Florida Atlantic University, Boca Raton, FL 33431, USA.
authors.forename: Gian Franco
authors.initials: GF
authors.lastname: Sferrazza
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
authors.forename: Pingping
authors.initials: P
authors.lastname: Jia
authors.forename: Sharon L
authors.initials: SL
authors.lastname: Lemanski
authors.forename: Gagani
authors.initials: G
authors.lastname: Athauda
authors.forename: Alyssa
authors.initials: A
authors.lastname: Stassi
authors.forename: Kristine
authors.initials: K
authors.lastname: Halager
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Maier
authors.forename: Elena
authors.initials: E
authors.lastname: Rueda-de-Leon
authors.forename: Amit
authors.initials: A
authors.lastname: Gupta
authors.forename: Syamalima
authors.initials: S
authors.lastname: Dube
authors.forename: Xupei
authors.initials: X
authors.lastname: Huang
authors.forename: Howard M
authors.initials: HM
authors.lastname: Prentice
authors.forename: Dipak K
authors.initials: DK
authors.lastname: Dube
authors.forename: Larry F
authors.initials: LF
authors.lastname: Lemanski
mesh_terms: D000558:Ambystoma mexicanum / Q000196:embryology* / Q000235:genetics; D000818:Animals; D030801:Animals, Genetically Modified; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D006321:Heart / Q000196:embryology* / Q000502:physiology; D009206:Myocardium / Q000378:metabolism*; D009210:Myofibrils / Q000502:physiology*; D012313:RNA / Q000235:genetics / Q000378:metabolism*; D013329:Structure-Activity Relationship; D020107:Troponin T / Q000235:genetics* / Q000378:metabolism*
chemical_list: D020107:Troponin T; D012313:RNA