Comparison of microarray and sage techniques in gene expression analysis of human glioblastoma.
To enhance glioblastoma (GB) marker discovery we compared gene expression in GB with human normal brain (NB) by accessing SAGE Genie web site and compared obtained results with published data. Nine GB and five NB SAGE-libraries were analyzed using the Digital Gene Expression Displayer (DGED), the results of DGED were tested by Northern blot analysis and RT-PCR of arbitrary selected genes. Review of available data from the articles on gene expression profiling by microarray-based hybridization showed as few as 35 overlapped genes with increased expression in GB. Some of them were identified in four articles, but most genes in three or even in two investigations. There was found also some differences between SAGE results of GB analysis. Digital Gene Expression Displayer approach revealed 676 genes differentially expressed in GB vs. NB with cut-off ratio: twofold change and P < or = 0.05. Differential expression of selectedgenes obtained by DGED was confirmed by Northern analysis and RT-PCR. Altogether, only 105 of 955 genes presented in published investigations were among the genes obtained by DGED. Comparison of the results obtained by microarrays and SAGE is very complicated because authors present only the most prominent differentially expressed genes. However, even available data give quite poor overlapping of genes revealed by microarrays. Some differences between results obtained by SAGE in different investigations can be explained by high dependence on the statistical methods used. As for now, the best solution to search for molecular tumor markers is to compare all available results and to select only those genes, which significant expression in tumor combined with very low expression in normal tissues was reproduced in several articles. 105 differentially expressed genes, common to both methods, can be included in the list of candidates for the molecular typing of GBs. Some genes, encoded cell surface or extra-cellular proteins may be useful for targeting gliomas with antibody-based therapy.
Kavsan
V M
VM
Institute of Molecular Biology and Genetics, Zabolotnogo Str. 150, 03143 Kyiv, Ukraine. kavsan@imbg.org.ua
Dmitrenko
V V
VV
Shostak
K O
KO
Bukreieva
T V
TV
Vitak
N Y
NY
Simirenko
O E
OE
Malisheva
T A
TA
Shamayev
M I
MI
Rozumenko
V D
VD
Zozulya
Y A
YA
D001932:Brain Neoplasms / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D020224:Expressed Sequence Tags; D015870:Gene Expression / Q000235:genetics*; D020869:Gene Expression Profiling; D015723:Gene Library; D005909:Glioblastoma / Q000235:genetics*; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018076:DNA, Complementary; D009363:Neoplasm Proteins