UVB-induced IL-18 production in human keratinocyte cell line NCTC 2544 through NF-kappaB activation.
In the present study, we investigated the implication of NF-kappaB in the production of pro-inflammatory cytokine IL-18 by human keratinocytes stimulated by UVB. We demonstrated that NCTC 2544 keratinocyte cell line irradiated by UVB enhanced the IL-18 mRNA and protein secretion under its bioactive form. Overexpression of IL-18 by UVB irradiation was accompanied by NF-kappaB transcription factor activation using specific IL-18 gene sequence corresponding to NF-kappaB DNA binding site. The relationship between these transcription factors and IL-18 expression was confirmed using curcumin and PDTC, two inhibitors of NF-kappaB. Our results show that UVB and curcumin or PDTC co-treatment led to a down-regulation of IL-18 expression associated with an inhibition of NF-kappaB DNA binding. Hence, our results demonstrated that this transcription factor is implicated in biologically active IL-18 production by human keratinocytes irradiated by UVB.
Grandjean-Laquerriere
Alexia
A
Laboratoire d'Immunologie et de Microbiologie, IPCM, EA3796, IFR53, UFR de Pharmacie, 1 rue du Maréchal Juin, 51096 Reims Cedex, France.
Antonicelli
Frank
F
Gangloff
Sophie C
SC
Guenounou
Moncef
M
Le Naour
Richard
R
D002478:Cells, Cultured; D003474:Curcumin / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000528:radiation effects; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D020382:Interleukin-18 / Q000096:biosynthesis* / Q000235:genetics; D015603:Keratinocytes / Q000378:metabolism* / Q000528:radiation effects*; D007962:Leukocytes / Q000378:metabolism / Q000528:radiation effects; D016328:NF-kappa B / Q000378:metabolism*; D011392:Proline / Q000031:analogs & derivatives / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D013859:Thiocarbamates / Q000494:pharmacology; D014466:Ultraviolet Rays
D020382:Interleukin-18; D016328:NF-kappa B; D012333:RNA, Messenger; D013859:Thiocarbamates; C066229:prolinedithiocarbamate; D007371:Interferon-gamma; D011392:Proline; D003474:Curcumin