title: Nucleoplasmic calcium is required for cell proliferation.
abstract: Ca(2+) signals regulate cell proliferation, but the spatial and temporal specificity of these signals is unknown. Here we use selective buffers of nucleoplasmic or cytoplasmic Ca(2+) to determine that cell proliferation depends upon Ca(2+) signals within the nucleus rather than in the cytoplasm. Nuclear Ca(2+) signals stimulate cell growth rather than inhibit apoptosis and specifically permit cells to advance through early prophase. Selective buffering of nuclear but not cytoplasmic Ca(2+) signals also impairs growth of tumors in vivo. These findings reveal a major physiological and potential pathophysiological role for nucleoplasmic Ca(2+) signals and suggest that this information can be used to design novel therapeutic strategies to regulate conditions of abnormal cell growth.
authors.affiliation: Department of Medicine, Yale University School of Medicine, New Haven, Connecticut 06520-8019, USA.
authors.forename: Michele A
authors.initials: MA
authors.lastname: Rodrigues
authors.forename: Dawidson A
authors.initials: DA
authors.lastname: Gomes
authors.forename: M Fatima
authors.initials: MF
authors.lastname: Leite
authors.forename: Wayne
authors.initials: W
authors.lastname: Grant
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Wing
authors.initials: W
authors.lastname: Lam
authors.forename: Yung-Chi
authors.initials: YC
authors.lastname: Cheng
authors.forename: Anton M
authors.initials: AM
authors.lastname: Bennett
authors.forename: Michael H
authors.initials: MH
authors.lastname: Nathanson
mesh_terms: D000818:Animals; D002118:Calcium / Q000502:physiology*; D020013:Calcium Signaling; D002453:Cell Cycle; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D049109:Cell Proliferation; D003593:Cytoplasm / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008938:Mitosis
chemical_list: D002118:Calcium