Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of molybdopterin synthase from Thermus thermophilus HB8.
Thermus thermophilus is a Gram-negative aerobic thermophilic eubacterium which can grow at temperatures ranging from 323 to 355 K. In addition to their importance in thermostability or adaptation strategies for survival at high temperatures, the thermostable enzymes in thermophilic organisms contribute to a wide range of biotechnological applications. The molybdenum cofactor in all three kingdoms consists of a tricyclic pyranopterin termed molybdopterin that bears the cis-dithiolene group responsible for molybdenum ligation. The crystals of molybdopterin synthase from T. thermophilus HB8 belong to the primitive monoclinic space group P2(1), with unit-cell parameters a = 33.94, b = 103.32, c = 59.59 A, beta = 101.3 degrees. Preliminary studies and molecular-replacement calculations reveal the presence of three monomers in the asymmetric unit.
Kanaujia
Shankar Prasad
SP
Bioinformatics Centre (Centre of Excellence in Structural Biology and Biocomputing), Indian Institute of Science, Bangalore 560 012, India.
Ranjani
Chellamuthu Vasuki
CV
Jeyakanthan
Jeyaraman
J
Ohmori
Miwa
M
Agari
Kazuko
K
Kitamura
Yoshiaki
Y
Baba
Seiki
S
Ebihara
Akio
A
Shinkai
Akeo
A
Kuramitsu
Seiki
S
Shiro
Yoshitsugu
Y
Sekar
Kanagaraj
K
Yokoyama
Shigeyuki
S
D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D011487:Protein Conformation; D013466:Sulfurtransferases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D016963:Thermus thermophilus / Q000201:enzymology*
D013466:Sulfurtransferases; C085585:molybdopterin synthase