Estimation of baculovirus titer based on viable cell size.
In this paper, a simple and rapid protocol for determination of baculovirus titers based on increasing viable insect cell size/diameter following virus infection is presented. There are different methods available for determining virus titers such as plaque assays end-point dilution, quantitative real-time polymerase chain reaction and flow cytometry. However, most of these methods are time consuming and labor intensive. The titer estimation method presented here can be completed in approximately 28 h from start to finish. In this method, the Vi-CELL (Beckman Coulter) was used to measure cell diameter change over a range of virus dilutions, following infection. The cell diameter change data were used to compute the virus titer using a statistical method called the method of moments that we have described previously.
Janakiraman
Vasantharajan
V
Department of Molecular Biology, Genentech Inc., 1 DNA way, South San Francisco, California 94080, USA.
Forrest
William F
WF
Seshagiri
Somasekar
S
D000818:Animals; D016367:Baculoviridae; D048429:Cell Size; D002470:Cell Survival; D007313:Insecta / Q000166:cytology / Q000821:virology*; D014773:Virology / Q000379:methods*