[Study on mannose-binding protein gene polymorphisms and susceptibility to pulmonary tuberculosis].
OBJECTIVE
To explore the association between the genetic polymorphisms of mannose-binding protein (MBP) alleles and susceptibility to pulmonary tuberculosis.


METHODS
125 pulmonary tuberculosis cases and 198 healthy controls were collected. A case-control study was conducted. Three structural gene mutations in exon 1 of MBP gene (codon 52, codon 54 and codon 57) were studied. Polymerase chain reaction with sequence-specific primers (PCR-SSP) was carried out in the polymorphism in MBP alleles. Information on related risk factors of tuberculosis was collected, using a pre-tested questionnaire. Univariate and multivariate logistic analyses were conducted with SPSS software package.


RESULTS
The frequencies of mutant heterozygote or homozygote of MBP-52, 54, 57 were 8.0%, 7.2% and 0.4% for cases and 5.3%, 4.3%, 0.5% for controls, respectively. The distribution of mutant genotypes of MBP did not show significant difference between tuberculosis patients and control by Mantel-Haenszel chi2 on sex. The univariate analysis demonstrated that body mass index, marital status, vaccinal vestige, bacillus of Calmette-Guerin vaccine immunization, contacted with pulmonary tuberculosis patients, familial traits were the risk factors of pulmonary tuberculosis. After adjusting those related environmental factors in the multivariate logistic analyses, the total MBP (MBP-52, MBP-54 and MBP-57) and MBP-52 heterozygote genotypes were significantly overrepresented in cases, with adjusted OR (95% CI) being 2.182 (1.058-4.499) and 2.574 (1.028-6.446).


CONCLUSIONS
Total MBP and MBP-52 mutant genotypes might be associated with the susceptibility to pulmonary tuberculosis.
Feng
Fu-min
FM
North China Coal Medical College, Tangshan 063000, China.
Guo
Mei
M
Liu
Qian
Q
Wang
Dong
D
Gao
Bao-xia
BX
Sun
Yong-hong
YH
An
Ya-chen
YC
Ji
Chun-mei
CM
D016022:Case-Control Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D037601:Mannose-Binding Lectin / Q000235:genetics*; D011110:Polymorphism, Genetic; D014397:Tuberculosis, Pulmonary / Q000235:genetics*
D037601:Mannose-Binding Lectin