title: The PGRS domain of Mycobacterium tuberculosis PE_PGRS Rv1759c antigen is an efficient subunit vaccine to prevent reactivation in a murine model of chronic tuberculosis.
abstract: A third of the world population is latently infected with Mycobacterium tuberculosis and many cases of active tuberculosis arise from latent bacilli reactivation. Thus, it is important to design new vaccines to prevent reactivation. Using an experimental model of chronic tuberculosis in B6D2F1 mice, we observed constant expression of Rv1759c antigen, a member of the PE_PGRS gene family, on the cell wall of phagocytosed mycobacteria by activated macrophages located in lung granulomas. This antigen induced production of IFN-gamma after stimulation of cell suspensions from mediastinal lymph nodes. It was notorius that chronic infected mice immunized with this antigen and treated with corticosterone to induce reactivation showed not change in colony forming units (CFU), compared with the significant bacilli increase in non-vaccinated mice treated with corticosterone. These results suggest that this antigen could play an important role in the immune response that maintains latent infection, and could therefore, be a good candidate as a new subunit vaccine to prevent disease reactivation.
authors.affiliation: Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de Mexico, Apartado Postal 70228, 04510 México D.F., Mexico.
authors.forename: Jaime
authors.initials: J
authors.lastname: Campuzano
authors.forename: Diana
authors.initials: D
authors.lastname: Aguilar
authors.forename: Kutzy
authors.initials: K
authors.lastname: Arriaga
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: León
authors.forename: Laura Patricia
authors.initials: LP
authors.lastname: Salas-Rangel
authors.forename: Jorge
authors.initials: J
authors.lastname: González-y-Merchand
authors.forename: Rogelio
authors.initials: R
authors.lastname: Hernández-Pando
authors.forename: Clara
authors.initials: C
authors.lastname: Espitia
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry* / Q000276:immunology* / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000276:immunology* / Q000378:metabolism; D002908:Chronic Disease; D004195:Disease Models, Animal; D005260:Female; D008565:Membrane Proteins / Q000737:chemistry* / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009169:Mycobacterium tuberculosis / Q000276:immunology; D032581:Tuberculosis Vaccines / Q000008:administration & dosage / Q000276:immunology*; D014397:Tuberculosis, Pulmonary / Q000276:immunology / Q000517:prevention & control*; D014611:Vaccination; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D008565:Membrane Proteins; C440642:PE-PGRS protein, Mycobacterium; D032581:Tuberculosis Vaccines; D022223:Vaccines, Subunit; C072681:Mycobacterium tuberculosis antigens