[Protective effect of donator liver ischemic preconditioning on posttransplant liver graft function in Chinese miniature pigs].
OBJECTIVE
To investigate the protective effect of the ischemia preconditioning of the donor liver on posttransplant liver graft function in Chinese miniature pigs.


METHODS
Twenty-five partially inbred Chinese miniature pigs were randomized into three groups, namely the normal control group, the ischemia-reperfusion group and ischemic preconditioning group. Biopsies of the liver graft were performed to analyze HSP70 expression by means of immunoblotting, and the changes of serum AST/ALT levels were assayed using an automated biochemical analyzer. Histopathological assessment was carried out to identify the hepatocyte injury using optical and transmission electron microscopy.


RESULTS
Ischemia preconditioning resulted in a notable increase in HSP70 expression and milder injury of the hepatocyte microstructure, whereas ischemia-reperfusion caused a significant increase of serum transaminases level (P<0.01) with declined HSP70 expression and obvious microstructural changes of the liver tissue.


CONCLUSIONS
Ischemic preconditioning can produce obvious protective effects on the donor liver, and positively regulates the expression of shock protein.
Pan
Ming-xin
MX
Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Kong
Fan-dong
FD
Wu
Zhi-feng
ZF
Zhang
Yi
Y
Wang
Hai-lan
HL
Li
Ai-hui
AH
Xie
Jin-ming
JM
Gao
Yi
Y
D000818:Animals; D005260:Female; D006085:Graft Survival / Q000502:physiology*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D019194:Ischemic Preconditioning; D008099:Liver / Q000098:blood supply* / Q000378:metabolism / Q000503:physiopathology; D016031:Liver Transplantation; D008297:Male; D011897:Random Allocation; D013552:Swine; D013556:Swine, Miniature
D018840:HSP70 Heat-Shock Proteins