title: [Effects of exogenous metallothionein on thermoresistance and SOD gene expression of dairy cattle].
abstract: To approach the effects of exogenous metallothionein (Zn-MT) on the thermoresistance and SOD gene expression of dairy cattle, an experiment was conducted with 28 lactating cows, which were randomly allocated to groups A, B, C and D, and supplemented with 0, 6.0, 12.0 and 16.0 mg Zn-MT x capita(-1), respectively, by intravenous injection. The results showed that the pulse, breath rate, and serum MDA content of the cows in groups B, C and D were lower (P < 0.05 or P < 0.01), while their milk yield, serum- and milk MT contents, blood GSH-PX activity, erythrocyte SOD activity, and SOD gene expression level were higher (P < 0.05 or P < 0.01) than those in group A. All the test indices of the cows in groups C and D were superior (P < 0.05 or P > 0.05) than those in group B, but no significant difference (P > 0.05) was observed between groups C and D. Exogenous Zn-MT had the best effects on the thermoresistance and SOD gene expression of dairy cattle 30 days after injection. All of these suggested that exogenous Zn-MT should be a physiologically active substance effective to the thermoresistance and SOD mRNA expression of dairy cattle, and presented time- and dose-dependent effects.
authors.affiliation: College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China. zhbin2119@hotmail.com
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.forename: Li-qun
authors.initials: LQ
authors.lastname: Xue
authors.forename: Li-li
authors.initials: LL
authors.lastname: Li
authors.forename: Xing-hou
authors.initials: XH
authors.lastname: De
authors.forename: Yu-guang
authors.initials: YG
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Hui-zhen
authors.initials: HZ
authors.lastname: Peng
authors.forename: Ding-fu
authors.initials: DF
authors.lastname: Xiao
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D002417:Cattle; D003612:Dairying; D005260:Female; D015870:Gene Expression; D006358:Hot Temperature; D008668:Metallothionein / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D008668:Metallothionein; D013482:Superoxide Dismutase