Immunohistochemistry in Drosophila: sections and whole mounts.
This chapter describes immunohistochemistry on Drosophila heads (brains) with respect to antigens involved in the circadian system. Two different methods have been successfully performed in several labs: immunolabeling on whole-mount brains and on cryostat sections of entire heads. Both methods are addressed here. The primary antisera can be detected by enzyme-labeled or fluorescence-labeled secondary antibodies. The advantages of the different methods are discussed.
Helfrich-Förster
Charlotte
C
Institut für Zoologie, Universität Regensburg, Germany.
D000818:Animals; D000906:Antibodies; D001921:Brain / Q000276:immunology / Q000502:physiology; D002940:Circadian Rhythm / Q000502:physiology*; D016616:Cryoultramicrotomy; D004210:Dissection; D004330:Drosophila / Q000276:immunology / Q000502:physiology*; D005456:Fluorescent Dyes; D007150:Immunohistochemistry / Q000379:methods*; D013194:Staining and Labeling; D016707:Tissue Fixation
D000906:Antibodies; D005456:Fluorescent Dyes