title: Effect of a xylitol and fluoride containing toothpaste on the remineralization of human enamel in vitro.
abstract: The aim of this study was to examine the effect of a fluoride and xylitol containing toothpaste on the remineralization of human enamel using Quantitative Light-induced Fluorescence (QLF). Human extracted teeth were cut longitudinally into three or four parts, and artificial subsurface lesion windows (2 mm x 3 mm) were created by immersion in demineralizing solution. Each enamel sample (n = 7) was treated for 3 min at 25 degrees C twice a day for consecutive 14 days with the slurry of a silica-based toothpaste 1) without F- and xylitol (blank), 2) with 500 ppm F- and 3) with 500 ppm F- and 5% xylitol toothpaste. In addition, we measured the remineralization ability of a commercially available toothpaste 4) with 500 ppm F-. The average fluorescence loss F (%) and lesion size (mm2) were determined with QLF. Another variable, delta Q, which was defined as the fluorescence loss integrated over the lesion size (%, mm2), was calculated. The results showed that the combination of 500 ppm F- and 5% xylitol toothpaste significantly (P < 0.05) recovered both the size and delta Q compared to the other groups. These findings suggested that inclusion of xylitol in fluoride toothpaste might be useful to enhance the remineralization in vivo.
authors.affiliation: Product Development Department, International Division, Lion Corporation, Tokyo, Japan. hirosano@lion.co.jp <hirosano@lion.co.jp>
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sano
authors.forename: Syozi
authors.initials: S
authors.lastname: Nakashima
authors.forename: Yupin
authors.initials: Y
authors.lastname: Songpaisan
authors.forename: Prathip
authors.initials: P
authors.lastname: Phantumvanit
mesh_terms: D000704:Analysis of Variance; D002327:Cariostatic Agents / Q000627:therapeutic use*; D003732:Dental Caries Activity Tests; D004338:Drug Combinations; D005453:Fluorescence; D005459:Fluorides / Q000627:therapeutic use*; D006801:Humans; D008027:Light; D018709:Statistics, Nonparametric; D017001:Tooth Demineralization / Q000628:therapy; D014089:Tooth Remineralization / Q000379:methods*; D014100:Toothpastes / Q000737:chemistry / Q000627:therapeutic use*; D014993:Xylitol / Q000627:therapeutic use*
chemical_list: D002327:Cariostatic Agents; D004338:Drug Combinations; D014100:Toothpastes; D005459:Fluorides; D014993:Xylitol