title: [Study on mannose-binding protein gene polymorphisms and susceptibility to pulmonary tuberculosis].
abstract: OBJECTIVE
  To explore the association between the genetic polymorphisms of mannose-binding protein (MBP) alleles and susceptibility to pulmonary tuberculosis.
  
  
  METHODS
  125 pulmonary tuberculosis cases and 198 healthy controls were collected. A case-control study was conducted. Three structural gene mutations in exon 1 of MBP gene (codon 52, codon 54 and codon 57) were studied. Polymerase chain reaction with sequence-specific primers (PCR-SSP) was carried out in the polymorphism in MBP alleles. Information on related risk factors of tuberculosis was collected, using a pre-tested questionnaire. Univariate and multivariate logistic analyses were conducted with SPSS software package.
  
  
  RESULTS
  The frequencies of mutant heterozygote or homozygote of MBP-52, 54, 57 were 8.0%, 7.2% and 0.4% for cases and 5.3%, 4.3%, 0.5% for controls, respectively. The distribution of mutant genotypes of MBP did not show significant difference between tuberculosis patients and control by Mantel-Haenszel chi2 on sex. The univariate analysis demonstrated that body mass index, marital status, vaccinal vestige, bacillus of Calmette-Guerin vaccine immunization, contacted with pulmonary tuberculosis patients, familial traits were the risk factors of pulmonary tuberculosis. After adjusting those related environmental factors in the multivariate logistic analyses, the total MBP (MBP-52, MBP-54 and MBP-57) and MBP-52 heterozygote genotypes were significantly overrepresented in cases, with adjusted OR (95% CI) being 2.182 (1.058-4.499) and 2.574 (1.028-6.446).
  
  
  CONCLUSIONS
  Total MBP and MBP-52 mutant genotypes might be associated with the susceptibility to pulmonary tuberculosis.
authors.affiliation: North China Coal Medical College, Tangshan 063000, China.
authors.forename: Fu-min
authors.initials: FM
authors.lastname: Feng
authors.forename: Mei
authors.initials: M
authors.lastname: Guo
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.forename: Bao-xia
authors.initials: BX
authors.lastname: Gao
authors.forename: Yong-hong
authors.initials: YH
authors.lastname: Sun
authors.forename: Ya-chen
authors.initials: YC
authors.lastname: An
authors.forename: Chun-mei
authors.initials: CM
authors.lastname: Ji
mesh_terms: D016022:Case-Control Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D037601:Mannose-Binding Lectin / Q000235:genetics*; D011110:Polymorphism, Genetic; D014397:Tuberculosis, Pulmonary / Q000235:genetics*
chemical_list: D037601:Mannose-Binding Lectin