title: Detection of the aminoglycosidestreptothricin resistance gene cluster ant(6)-sat4-aph(3 ')-III in commensal viridans group streptococci.
abstract: High-level aminoglycoside resistance was assessed in 190 commensal erythromycin-resistant alpha-hemolytic streptococcal strains. Of these, seven were also aminoglycoside-resistant: one Streptococcus mitis strain was resistant to high levels of kanamycin and carried the aph(3 ')-III gene, four S. mitis strains were resistant to high levels of streptomycin and lacked aminoglycoside-modifying enzymes, and two S. oralis strains that were resistant to high levels of kanamycin and streptomycin harbored both the aph(3 ')-III and the ant(6) genes. The two S. oralis strains also carried the ant(6)-sat4- aph(3 ' ')-III aminoglycoside-streptothricin resistance gene cluster, but it was not contained in a Tn5405-like structure. The presence of this resistance gene cluster in commensal streptococci suggests an exchange of resistance genes between these bacteria and enterococci or staphylococci.
authors.affiliation: Department of Microbiology, Preventive Medicine and Public Health, Zaragoza University School of Medicine, Spain.
authors.forename: Paula
authors.initials: P
authors.lastname: Cerdá
authors.forename: Pilar
authors.initials: P
authors.lastname: Goñi
authors.forename: Leticia
authors.initials: L
authors.lastname: Millán
authors.forename: Carmen
authors.initials: C
authors.lastname: Rubio
authors.forename: Rafael
authors.initials: R
authors.lastname: Gómez-Lus
mesh_terms: D000617:Aminoglycosides / Q000494:pharmacology*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D004697:Endocarditis, Bacterial / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D013290:Streptococcal Infections / Q000382:microbiology; D034363:Viridans Streptococci / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism
chemical_list: D000617:Aminoglycosides; D004269:DNA, Bacterial