Platelet-rich plasma: growth factors and pro- and anti-inflammatory properties.
BACKGROUND
Platelet-rich plasma (PRP) promotes regeneration of bone, presumably through the action of concentrated growth factors. However, it is not clear how PRP affects the inflammatory response. The purpose of this study was to analyze the growth factors in PRP and to study the effects of PRP on monocyte cytokine release and lipoxin A(4) (LXA(4)) generation.


METHODS
PRP was prepared from healthy donors. Platelet-derived growth factor (PDGF)-AB, PDGF-BB, transforming growth factor-beta1, insulin-like growth factor-I, fibroblast growth factor-basic (FGF-b), epidermal growth factor (EGF), vascular endothelial growth factor, interleukin-12 (p40/70), and regulated on activation, normal T-cell expressed and secreted (RANTES) levels were evaluated by enzyme-linked immunosorbent assay and bead-based multiplexing. Peripheral blood monocytes were isolated and cultured with or without PRP. Cytokine, chemokine, and LXA(4) levels as well as monocyte chemotactic migration were analyzed.


RESULTS
Growth factors were increased significantly in PRP compared to whole blood (WB) and platelet-poor plasma. Monocyte chemotactic protein-1 (MCP-1) was suppressed significantly by PRP, whereas RANTES was increased significantly in monocyte cultures. LXA(4) levels were significantly higher in PRP compared to WB. PRP stimulated monocyte chemotaxis in a dose-dependent fashion, whereas RANTES, in part, was responsible for PRP-mediated monocyte migration.


CONCLUSIONS
PRP is a rich source of growth factors and promoted significant changes in monocyte-mediated proinflammatory cytokine/chemokine release. LXA(4) was increased in PRP, suggesting that PRP may suppress cytokine release, limit inflammation, and, thereby, promote tissue regeneration.
El-Sharkawy
Hesham
H
Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, Boston, MA 02118, USA.
Kantarci
Alpdogan
A
Deady
Jennifer
J
Hasturk
Hatice
H
Liu
Hongsheng
H
Alshahat
Mohammad
M
Van Dyke
Thomas E
TE
D000328:Adult; D018946:Chemokine CCL5 / Q000097:blood; D002634:Chemotaxis, Leukocyte / Q000502:physiology; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000502:physiology*; D044045:Lipoxins / Q000032:analysis; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D010976:Platelet Count; D053657:Platelet-Rich Plasma / Q000737:chemistry* / Q000378:metabolism
D018946:Chemokine CCL5; D016207:Cytokines; D036341:Intercellular Signaling Peptides and Proteins; D044045:Lipoxins; C040527:lipoxin A4