title: T cell repertoire diversity is required for relapses in myelin oligodendrocyte glycoprotein-induced experimental autoimmune encephalomyelitis.
abstract: Comparison of TCRalphabeta repertoires of myelin oligodendrocyte glycoprotein (MOG)-specific T lymphocytes in C57BL/6 and TdT-deficient littermates (TdT(-/-)) generated during experimental autoimmune encephalomyelitis (EAE) highlights a link between a diversified TCRalphabeta repertoire and EAE relapses. At the onset of the disease, the EAE-severity is identical in TdT(+/-) and TdT(-/-) mice and the neuropathologic public MOG-specific T cell repertoires express closely similar public Valpha-Jalpha and Vbeta-Jbeta rearrangements in both strains. However, whereas TdT(+/+) and TdT(+/-) mice undergo successive EAE relapses, TdT(-/-) mice recover definitively and the lack of relapses does not stem from dominant regulatory mechanisms. During the first relapse of the disease in TdT(+/-) mice, new public Valpha-Jalpha and Vbeta-Jbeta rearrangements emerge that are distinct from those detected at the onset of the disease. Most of these rearrangements contain N additions and are found in CNS-infiltrating T lymphocytes. Furthermore, CD4(+) T splenocytes bearing these rearrangements proliferate to the immunodominant epitope of MOG and not to other immunodominant epitopes of proteolipid protein and myelin basic protein autoantigens, excluding epitope spreading to these myelin proteins. Thus, in addition to epitope spreading, a novel mechanism involving TCRalphabeta repertoire diversification contributes to autoimmune progression.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, Unité 277, Institut Pasteur, Paris, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Fazilleau
authors.forename: Cécile
authors.initials: C
authors.lastname: Delarasse
authors.forename: Iris
authors.initials: I
authors.lastname: Motta
authors.forename: Simon
authors.initials: S
authors.lastname: Fillatreau
authors.forename: Marie-Lise
authors.initials: ML
authors.lastname: Gougeon
authors.forename: Philippe
authors.initials: P
authors.lastname: Kourilsky
authors.forename: Danielle
authors.initials: D
authors.lastname: Pham-Dinh
authors.forename: Jean M
authors.initials: JM
authors.lastname: Kanellopoulos
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000473:pathology; D002478:Cells, Cultured; D004253:DNA Nucleotidylexotransferase / Q000502:physiology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000209:etiology* / Q000276:immunology; D015332:Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D016056:Immunodominant Epitopes; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein / Q000276:immunology*; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments / Q000276:immunology; D012008:Recurrence; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D016056:Immunodominant Epitopes; C571327:Mog protein, mouse; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments; D004253:DNA Nucleotidylexotransferase