Preparation of an ion-exchangeable polymer bead wrapped with bilayer membrane structures for high performance liquid chromatography.
We synthesized a chromatographic packing material that has a non-covalently attached dihexadecyl phosphate (DHP) bilayer membrane structure on a CA08S, a nonporous-type cationic polymer bead with a diameter ranging from 11 to 14 microm. Confocal fluorescence microscopic and differential scanning calorimetric analyses of the DHP-CA08S complex revealed that the DHP bilayer membrane structures were formed on the surface of the CA08S polymer beads. When the functionality of the DHP-CA08S complex was evaluated in the ion-exchange HPLC of proteins, the retention behavior of the proteins on the DHP-CA08S complex column totally mirrored the anionic property of the DHP bilayer membrane surface, not the cationic property of the CA08S bead. Methylene blue (MB) was eluted from the DHP-CA08S complex column in the isocratic elution mode, but not at all from a CK08S column, a styrene-divinylbenzene based cation-exchange polymer. When the column temperature was elevated from 50 to 60 degrees C, the peak shape of MB on the DHP-CA08S complex column became fairly sharp without a change in its peak area, which mirrored the characteristic phase transition of the DHP bilayer membrane formed on the DHP-CA08S complex.
Haratake
Mamoru
M
Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki 852-8521, Japan. haratake@nagasaki-u.ac.jp <haratake@nagasaki-u.ac.jp>
Hidaka
Shuko
S
Ono
Masahiro
M
Nakayama
Morio
M
D002152:Calorimetry, Differential Scanning; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007475:Ion Exchange Resins; D008051:Lipid Bilayers; D018613:Microscopy, Confocal
D007475:Ion Exchange Resins; D008051:Lipid Bilayers