title: The effect of milk basic protein supplementation on bone metabolism during training of young thoroughbred racehorses.
abstract: BACKGROUND
  In laboratory animals, man and cell culture experiments, milk basic protein was reported to suppress bone resorption and promote bone formation. However, no studies in horses have previously examined the effect of milk basic protein.
  
  
  OBJECTIVE
  To evaluate the effect of milk basic protein supplementation on bone metabolism in young Thoroughbred horses in training.
  
  
  METHODS
  Twenty 2-year-old horses in training were used for 90 days in this study. The treatment group was fed a basal diet with 1 g of milk basic protein and the control group a basal diet only. Blood samples were collected on Days 0, 45 and 90 to determine serum calcium (Ca) and biochemical markers of bone metabolism. Radiographs were taken at the start and end of the study to determine radiographic bone aluminium equivalence (RBAE).
  
  
  RESULTS
  Serum osteocalcin (OC) was significantly higher at Day 45 after the beginning of the study in the treatment group compared to that in the control group. The treatment group showed a greater increase in the total RBAE change at the end of this study compared to that in the control group. However, there were no significant differences in serum Ca and carboxy-terminal telopeptide of type I collagen (ICTP) between groups.
  
  
  CONCLUSIONS
  These findings provide preliminary evidence that milk basic protein has an effect on bone formation in 2-year-old Thoroughbred horses in training. However, further studies in larger groups of horses are now required to substantiate our findings.
authors.affiliation: Equine Science Division, Hidaka Training and Research Center, Japan Racing Association, 535-13, Aza-Nishicha, Urakawa-cho, Urakawa- gun, Hokkaido, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Inoue
authors.forename: Y
authors.initials: Y
authors.lastname: Asai
authors.forename: M
authors.initials: M
authors.lastname: Tomita
authors.forename: K
authors.initials: K
authors.lastname: Kuribara
authors.forename: M
authors.initials: M
authors.lastname: Kobayashi
authors.forename: M
authors.initials: M
authors.lastname: Kaneko
authors.forename: Y
authors.initials: Y
authors.lastname: Toba
mesh_terms: D000818:Animals; D015519:Bone Density / Q000187:drug effects / Q000502:physiology; D001842:Bone and Bones / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000378:metabolism*; D002118:Calcium / Q000097:blood*; D004044:Dietary Proteins / Q000008:administration & dosage*; D005260:Female; D006736:Horses / Q000378:metabolism*; D008297:Male; D008894:Milk Proteins / Q000008:administration & dosage; D015675:Osteocalcin / Q000097:blood; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology; D010805:Physical Conditioning, Animal / Q000502:physiology*; D011859:Radiography; D013997:Time Factors
chemical_list: D004044:Dietary Proteins; D008894:Milk Proteins; D015675:Osteocalcin; D002118:Calcium