title: Crystallization and preliminary X-ray crystallographic analysis of the heterodimeric crotoxin complex and the isolated subunits crotapotin and phospholipase A2.
abstract: Crotoxin, a potent neurotoxin from the venom of the South American rattlesnake Crotalus durissus terrificus, exists as a heterodimer formed between a phospholipase A(2) and a catalytically inactive acidic phospholipase A(2) analogue (crotapotin). Large single crystals of the crotoxin complex and of the isolated subunits have been obtained. The crotoxin complex crystal belongs to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 38.2, b = 68.7, c = 84.2 A, and diffracted to 1.75 A resolution. The crystal of the phospholipase A(2) domain belongs to the hexagonal space group P6(1)22 (or its enantiomorph P6(5)22), with unit-cell parameters a = b = 38.7, c = 286.7 A, and diffracted to 2.6 A resolution. The crotapotin crystal diffracted to 2.3 A resolution; however, the highly diffuse diffraction pattern did not permit unambiguous assignment of the unit-cell parameters.
authors.affiliation: Department of Physics, IBILCE/UNESP, Cristóvão Colombo 2265, CEP 15054-000, São José do Rio Preto, SP, Brazil.
authors.forename: K F
authors.initials: KF
authors.lastname: Santos
authors.forename: M T
authors.initials: MT
authors.lastname: Murakami
authors.forename: A C O
authors.initials: AC
authors.lastname: Cintra
authors.forename: M H
authors.initials: MH
authors.lastname: Toyama
authors.forename: S
authors.initials: S
authors.lastname: Marangoni
authors.forename: V P
authors.initials: VP
authors.lastname: Forrer
authors.forename: J R
authors.initials: JR
authors.lastname: Brandão Neto
authors.forename: I
authors.initials: I
authors.lastname: Polikarpov
authors.forename: R K
authors.initials: RK
authors.lastname: Arni
mesh_terms: D003439:Crotoxin / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D019281:Dimerization; D010741:Phospholipases A / Q000737:chemistry*; D054467:Phospholipases A2; D011487:Protein Conformation
chemical_list: D003439:Crotoxin; D010741:Phospholipases A; D054467:Phospholipases A2