title: Development of differential sensitivity of CaOv ovarian adenocarcinoma cells to antitumor agents under conditions of hypoxia.
abstract: We studied the role of VEGF signal pathway in autocrine regulation of tumor cell growth and survival under conditions of hypoxia. Hypoxia-resistant CaOv/H substrain with high level of VEGF-A secretion was obtained by long-term culturing of CaOv ovarian adenocarcinoma cells with CoCl2 (hypoxia inductor). VEGF-A directly participates in autocrine regulation of CaOv cell growth, including the maintenance of cell growth under conditions of hypoxia or cytostatic treatment. On the other hand, CaOv/H cells retain high apoptotic potential and are characterized by high expression of p27Kip1 (cyclin-dependent kinase inhibitor), which attests to possible involvement of this inhibitor into the regulation of apoptotic response of cells under conditions of hypoxia.
authors.affiliation: Russian State Medical University. opavlichenko@yandex.ru
authors.forename: O V
authors.initials: OV
authors.lastname: Pavlichenko
authors.forename: A N
authors.initials: AN
authors.lastname: Shishkin
authors.forename: E V
authors.initials: EV
authors.lastname: Stepanova
authors.forename: T K
authors.initials: TK
authors.lastname: Dubovaya
authors.forename: M A
authors.initials: MA
authors.lastname: Krasil'nikov
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D015687:Cell Hypoxia / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003035:Cobalt / Q000494:pharmacology; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D042461:Vascular Endothelial Growth Factor A; D003035:Cobalt; C018021:cobaltous chloride