Evaluation of mutagenic activity in supply water at three sites in the state of Rio Grande do Sul, Brazil.
In this study, the Salmonella/microsome assay, using the micro-suspension method, was utilized to evaluate water for public supply at three sites in Rio Grande do Sul. The first site selected was in an area under industrial influence and the others were in non-industrial reference areas. Based on 40 L samples of raw water and/or after conventional treatment, compounds were extracted with XAD4 resins using natural and acidic pH, and the extracts were analyzed in the TA98 and TA100 strains with and without S9. Raw water extracts in the industrial region induced 27.4 revertants/L (rev/L) for TA100+S9 up to 226.3 rev/L for TA100-S9, both for acidic pH extracts. After conventional treatment the responses varied from 20.6 rev/L (TA98-S9) for natural pH extracts to 755.5 rev/L (TA98-S9) for acidic pH extracts. For acidic extracts obtained from reference site samples, the response, with metabolic activation only, ranged from negative to minimal. Direct mutagenic responses in acidic extracts may be elevated in treated extracts, compared with raw water extracts, influenced by the presence of by-products of the chlorination process. However, the mutagenicity observed in the treated water extracts in the industrial area increased and reflected mainly the combination of directly and indirectly acting compounds in the source waters, that are heavily influenced by anthropogenic factors.
Pereira
Tatiana da Silva
Tda S
Programa de Pesquisas Ambientais, Fundação Estadual de Proteção Ambiental Henrique Luis Roessler (FEPAM), Av. Dr. Salvador França, 1707 CEP 90690-000, Porto Alegre, RS, Brazil. ecorisco@fepam.rs.gov.br
Rocha
Jocelita Aparecida Vaz
JA
Duccatti
Adriana
A
Silveira
Graziella Antunes
GA
Pastoriza
Thienne Flores
TF
Bringuenti
Lizandra
L
Vargas
Vera Maria Ferrão
VM
D000818:Animals; D001938:Brazil; D066298:In Vitro Techniques; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D009152:Mutagenicity Tests; D009153:Mutagens / Q000032:analysis* / Q000633:toxicity; D051381:Rats; D012475:Salmonella / Q000187:drug effects / Q000235:genetics; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000633:toxicity; D018508:Water Purification; D014881:Water Supply / Q000032:analysis*
D009153:Mutagens; D014874:Water Pollutants, Chemical