title: Alterations in mRNA expression of myelin proteins in the sciatic nerves and brains of streptozotocin-induced diabetic rats.
abstract: Diabetic neuropathy is the most common complication of diabetes. We examined the levels and the mRNA expression of myelin proteins in the sciatic nerves and the brains of streptozotocin-induced diabetic rats. The diabetic rats exhibited a decrease in body weight, elevation of the blood glucose level and a decrease in motor nerve conduction velocity at 2 weeks after streptozotocin injection. In the sciatic nerves of diabetic rats, the level of P0 protein and its mRNA expression were markedly reduced at 20 weeks after the injection. In the brains, the levels of proteolipid protein and myelin-associated glycoprotein and their mRNA expression were selectively decreased at 20 weeks after the injection. This affected expression of myelin proteins was found even when no histological abnormalities were detectable. Considering the functional significance of myelin proteins, this impairment of protein expression is possibly involved in the pathogenesis of diabetic neuropathy, including that in brain disorders.
authors.affiliation: Department of Biochemistry, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara-shi, Kanagawa, Japan.
authors.forename: Rei
authors.initials: R
authors.lastname: Kawashima
authors.forename: Hisako
authors.initials: H
authors.lastname: Kojima
authors.forename: Kazuo
authors.initials: K
authors.lastname: Nakamura
authors.forename: Atsushi
authors.initials: A
authors.lastname: Arahata
authors.forename: Yoshikuni
authors.initials: Y
authors.lastname: Fujita
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Tokuyama
authors.forename: Toyokazu
authors.initials: T
authors.lastname: Saito
authors.forename: Sen-Ichi
authors.initials: S
authors.lastname: Furudate
authors.forename: Tadashi
authors.initials: T
authors.lastname: Kurihara
authors.forename: Saburo
authors.initials: S
authors.lastname: Yagishita
authors.forename: Kunio
authors.initials: K
authors.lastname: Kitamura
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Tamai
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D001923:Brain Chemistry / Q000187:drug effects*; D015342:DNA Probes; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D008297:Male; D009046:Motor Neurons / Q000187:drug effects; D018993:Myelin P0 Protein / Q000378:metabolism; D009185:Myelin Proteins / Q000096:biosynthesis*; D009431:Neural Conduction / Q000187:drug effects; D010525:Peripheral Nerves / Q000503:physiopathology; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000187:drug effects / Q000378:metabolism*
chemical_list: D015342:DNA Probes; D018076:DNA, Complementary; D018993:Myelin P0 Protein; D009185:Myelin Proteins; D012333:RNA, Messenger