title: Analgesic activity of Ugni molinae (murtilla) in mice models of acute pain.
abstract: Leaf extracts of Ugni molinae Turcz. (Myrtaceae) are used in Chilean folk medicine as analgesic and anti-inflammatory. The antinociceptive effect of dichloromethane (DCM), ethyl acetate (EA) and methanol (ME) leaf extracts was assessed by intraperitoneal, oral and topical administration in writhing, tail flick, and tail formalin tests in mice. The extracts showed a dose-dependent antinociceptive activity in all the assays under different administration routes. The ED(50) values for the different tests for the DCM, EA, ME extract and reference drug (ibuprofen) were as follows. Writhing test in acetic acid (i.p. administration): 0.21, 0.37, 1.37 and 0.85mg/kg, respectively; tail flick test (oral administration): 199, 189, 120 and 45.9mg/kg. The EC(50) values for tail flick test were (topical administration): 2.0, 0.35, 1.4 and 8.2% (w/v), respectively; and the topical analgesic effects were (formalin assay) 75.5, 77.5, 31.6 and 76.5%, respectively. Ugni molinae extracts produce antinociception in chemical and thermal pain models through a mechanism partially linked to either lipooxygenase and/or cyclooxygenase via the arachidonic acid cascade and/or opioid receptors. Flavonoid glycosides and triterpenoids have been isolated from the plant and can be associated with the observed effect. Our results corroborate the analgesic effects of Ugni molinae, and justify its traditional use for treating pain.
authors.affiliation: Laboratory of Natural Products, Department of Pharmacological and Toxicological Chemistry, Faculty of Chemical and Pharmaceutical Sciences, University of Chile, Casilla 233, Santiago 1, Chile. cdelpor@uchile.cl <
authors.forename: C
authors.initials: C
authors.lastname: Delporte
authors.forename: N
authors.initials: N
authors.lastname: Backhouse
authors.forename: V
authors.initials: V
authors.lastname: Inostroza
authors.forename: M C
authors.initials: MC
authors.lastname: Aguirre
authors.forename: N
authors.initials: N
authors.lastname: Peredo
authors.forename: X
authors.initials: X
authors.lastname: Silva
authors.forename: R
authors.initials: R
authors.lastname: Negrete
authors.forename: H F
authors.initials: HF
authors.lastname: Miranda
mesh_terms: D000208:Acute Disease; D000700:Analgesics / Q000737:chemistry / Q000627:therapeutic use*; D000818:Animals; D005419:Flavonoids / Q000032:analysis; D006027:Glycosides / Q000032:analysis; D007052:Ibuprofen / Q000627:therapeutic use; D008297:Male; D051379:Mice; D027822:Myrtaceae; D010146:Pain / Q000188:drug therapy*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use; D018515:Plant Leaves; D014315:Triterpenes / Q000032:analysis
chemical_list: D000700:Analgesics; D005419:Flavonoids; D006027:Glycosides; D010936:Plant Extracts; D014315:Triterpenes; D007052:Ibuprofen