Genes required for mitotic spindle assembly in Drosophila S2 cells.
The formation of a metaphase spindle, a bipolar microtubule array with centrally aligned chromosomes, is a prerequisite for the faithful segregation of a cell's genetic material. Using a full-genome RNA interference screen of Drosophila S2 cells, we identified about 200 genes that contribute to spindle assembly, more than half of which were unexpected. The screen, in combination with a variety of secondary assays, led to new insights into how spindle microtubules are generated; how centrosomes are positioned; and how centrioles, centrosomes, and kinetochores are assembled.
Goshima
Gohta
G
Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California, San Francisco, 600 16th Street, San Francisco, CA 94158, USA.
Wollman
Roy
R
Goodwin
Sarah S
SS
Zhang
Nan
N
Scholey
Jonathan M
JM
Vale
Ronald D
RD
Stuurman
Nico
N
D000818:Animals; D002460:Cell Line; D018385:Centrosome / Q000378:metabolism / Q000648:ultrastructure; D002875:Chromosomes / Q000502:physiology / Q000648:ultrastructure; D029721:Drosophila Proteins / Q000235:genetics* / Q000502:physiology*; D004331:Drosophila melanogaster; D017344:Genes, Insect; D007091:Image Processing, Computer-Assisted; D018386:Kinetochores / Q000378:metabolism; D008677:Metaphase; D008870:Microtubules / Q000378:metabolism; D008938:Mitosis; D010641:Phenotype; D034622:RNA Interference; D008941:Spindle Apparatus / Q000235:genetics* / Q000378:metabolism* / Q000648:ultrastructure; D014404:Tubulin / Q000378:metabolism
D029721:Drosophila Proteins; D014404:Tubulin