Estimation of systemic complement C3 activity in age-related macular degeneration.
OBJECTIVE
To determine the role of systemic complement activation in the pathogenesis of age-related macular degeneration and to examine whether serum C3a des Arg reflects systemic complement activation, independent of individual complement component levels.


METHODS
Plasma complement C3a des Arg levels and a single nucleotide polymorphism at position 402 of the complement factor H gene (CFH) were determined in 3 groups of subjects: 42 subjects with early age-related maculopathy, 42 subjects with neovascular (wet) age-related macular degeneration, and a control group of 38 subjects with no clinical evidence of age-related changes at the macula.


RESULTS
The median (range) of plasma complement C3a des Arg levels in the age-related maculopathy and neovascular age-related macular degeneration groups were 52.6 (2.8-198.1) ng/mL and 60.9 (3.1-173.1) ng/mL, respectively. The levels were significantly raised compared with the control group (n = 38), which had a median (range) plasma complement C3a des Arg level of 40.3 (6.1-81.7) ng/mL (analysis of variance, P = .02). The concentration of plasma C3a des Arg did not differ significantly between those with different CFH genotypes (P = .07).


CONCLUSIONS
Systemic activation of the complement system may contribute to the pathogenesis of age-related macular degeneration independent of CFH polymorphism.


CONCLUSIONS
The results of this study may be relevant to aiming new treatment strategies toward reducing systemic low-grade inflammation.
Sivaprasad
Sobha
S
Laser and Retinal Research Unit, King's College Hospital, King's College London, England. senswathi@aol.com
Adewoyin
Temi
T
Bailey
Tracey A
TA
Dandekar
Sam S
SS
Jenkins
Sharon
S
Webster
Andrew R
AR
Chong
Ngaihang Victor
NV
D020256:Choroidal Neovascularization / Q000097:blood* / Q000209:etiology / Q000235:genetics; D003167:Complement Activation / Q000502:physiology*; D015926:Complement C3a / Q000032:analysis*; D017242:Complement Factor H / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005838:Genotype; D006801:Humans; D008268:Macular Degeneration / Q000097:blood* / Q000209:etiology / Q000235:genetics; D020641:Polymorphism, Single Nucleotide
C493495:CFH protein, human; C044541:complement C3a, des-Arg-(77)-; D015926:Complement C3a; D017242:Complement Factor H