Improved 16S rRNA-targeted probe set for analysis of sulfate-reducing bacteria by fluorescence in situ hybridization.
An updated dataset of in silico specificities for 54 previously published 16S rRNA-targeted oligonucleotides was assembled to provide guidance for reliable fluorescence in situ hybridization (FISH) analysis of sulfate-reducing bacteria. Additionally, six new FISH probes were developed for major deltaproteobacterial taxa, including a probe trio targeting most Deltaproteobacteria and Gemmatimonadetes.
Lücker
Sebastian
S
Department of Microbial Ecology, Faculty of Life Sciences, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria.
Steger
Doris
D
Kjeldsen
Kasper Urup
KU
MacGregor
Barbara J
BJ
Wagner
Michael
M
Loy
Alexander
A
D030561:Databases, Nucleic Acid; D020564:Deltaproteobacteria / Q000235:genetics / Q000302:isolation & purification; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D015345:Oligonucleotide Probes; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013431:Sulfates / Q000378:metabolism*; D016999:Sulfur-Reducing Bacteria / Q000235:genetics / Q000302:isolation & purification*
D015345:Oligonucleotide Probes; D012336:RNA, Ribosomal, 16S; D013431:Sulfates