A new multiplex PCR for easy screening of methicillin-resistant Staphylococcus aureus SCCmec types I-V.
A multiplex PCR with four primer-pairs was designed to identify the five main known SCCmec types. A clear and easily discriminated band pattern was obtained for all five types. The SCCmec type was identified for 98% of 312 clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA). SCCmec type IV was by far the most common SCCmec type among both hospital- and community-acquired MRSA isolates in Denmark.
Boye
K
K
Department of Clinical Microbiology, Hvidovre Hospital, Hvidovre, Denmark. Kit.Boye@hvh.regionh.dk
Bartels
M D
MD
Andersen
I S
IS
Møller
J A
JA
Westh
H
H
D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D002876:Chromosomes, Bacterial / Q000235:genetics; D017714:Community-Acquired Infections / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology; D003428:Cross Infection / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology; D017931:DNA Primers; D003718:Denmark / Q000453:epidemiology; D006801:Humans; D008403:Mass Screening / Q000379:methods; D016106:Methicillin Resistance / Q000235:genetics*; D046915:Penicillin-Binding Proteins; D016133:Polymerase Chain Reaction / Q000379:methods*; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification* / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification
D001426:Bacterial Proteins; D017931:DNA Primers; D046915:Penicillin-Binding Proteins; C487789:mecA protein, Staphylococcus aureus