[Expression and identification of Cry 1Ie in Bacillus coli].
OBJECTIVE
In this study, Cry1Ie expressed protein in the transgenic Cry1Ie maize was used as a model protein to establish a technical platform for the safety assessment of other genetically modified plants.


METHODS
Bacillus coli expression system was used to express Cry1Ie protein and then the expressed protein was purified by SDS-PAGE. The equivalence of Cry1Ie proteins expressed in Bacillus coli and in transgenic Cry1Ie maize was analyzed by their immunorecognition and bioactivity.


RESULTS
Cry1Ie protein was highly expressed in Bacillus coli and could be segregated and purified by SDS-PAGE. Cry1Ie expressed protein in Bacillus coli is substantially equivalent to that of maize genetically modified with Cry1Ie in tests of immunorecognition western blot and bioactivity in target pest.


CONCLUSIONS
The established technical platform of external Cry1Ie expressed protein can be applied to assess the food safety of genetically modified plants.
Xu
Haibin
H
Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing.
Zhang
Xiaoxia
X
Li
Fang
F
Wang
Guoying
G
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D003257:Consumer Product Safety; D004731:Endotoxins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D006460:Hemolysin Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D030821:Plants, Genetically Modified / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D003313:Zea mays / Q000235:genetics
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; D011994:Recombinant Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis