[Detection of rhodococcus equi by microbiological culture and by polymerase chain reaction in samples of tracheobronchial secretions of foals].
The goal of the present study was to investigate whether new PCR-methods would improve diagnostic of R. equi. In a first step, sensitivity and specificity of the PCR-methods in respect to the"gold standard" microbiological culture were determined. Secondly, sensitivity and specificity of both microbiological methods were evaluated in respect to the clinical diagnosis. The tracheobronchial secretions of 48 foals with pulmonary abscesses and of 37 healthy foals were evaluated by bacteriological culture as well as by four PCR-methods: aceA-, ideR-, vapA- and VP-PCR. In respect to the"gold standard" microbiological culture, the sensitivity of most PCR methods lay between 63.9 and 69.4 % except the vapA-PCR (27.8 %). The specificity of all PCR methods in this comparison was between 98 to 100 %. In this analysis, clinical diagnosis had a low sensitivity (66.7 %) and a low specificity (51.0 %). In respect to the clinical diagnosis, microbiological culture sensitivity was 50.0 % and specificity 67.7 9%. In this analysis, sensitivity rates of aceA-, ideR and VP-PCR methods lay between 33.3 and 37.5 %, sensitivity of the vapA-PCR was lower (10.4 %). The specificity of all PCR methods ranged from 78.4 to 86.5 %. In conclusion, these results show that the diagnostic potential of the microbiological methods"Culture" and "PCR" is different and that for the diagnosis of R. equi-pneumonia in foals the combination of microbiological culture with PCR should be used for examination of samples of the airways of foals.
Venner
Monica
M
Klinik für Pferde, Stiftung Tierärztliche Hochschule Hannover. monica.venner@tiho-hannover.de
Heyers
Paul
P
Strutzberg-Minder
Katrin
K
Lorenz
Nicole
N
Verspohl
Jutta
J
Klug
Erich
E
D000193:Actinomycetales Infections / Q000175:diagnosis / Q000662:veterinary*; D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D015169:Colony Count, Microbial / Q000379:methods / Q000662:veterinary; D005260:Female; D006734:Horse Diseases / Q000175:diagnosis*; D006736:Horses; D008168:Lung / Q000382:microbiology; D008169:Lung Abscess / Q000382:microbiology; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D016929:Rhodococcus equi / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014132:Trachea / Q000382:microbiology