Effect of resuscitation with 21% oxygen and 100% oxygen on NMDA receptor binding characteristics following asphyxia in newborn piglets.
The present study investigated the effect of reventilation with 21% and 100% oxygen following asphyxia in newborn piglets on NMDA receptor binding characteristics, Na(+), K(+)-ATPase activity, and lipid peroxidation. After achieving a heart rate less than 60 beats per minute, asphyxiated piglets were reventilated with 21% oxygen or 100% oxygen. (3)[H]MK-801 binding showed the Bmax in the 21% and 100% groups to be 1.53 +/- 0.43 and 1.42 +/- 0.35 pmol/mg protein (p = ns). Values for Kd were 4.56 +/- 1.29 and 4.17 +/- 1.05 nM (p = ns). Na(+), K(+)-ATPase activity in the 21% and 100% groups were 23.5 +/- 0.9 and 24.4 +/- 3.9 micromol Pi/mg protein/h (p = ns). Conjugated dienes (0.05 +/- 0.02 vs. 0.07 +/- 0.03 micromol/g brain) and fluorescent compounds (0.54 +/- 0.05 vs. 0.78 +/- 0.19 microg quinine sulfate/g brain), were similar in both groups (p = ns). Though lipid peroxidation products trended higher in the 100% group, these data show that NMDA receptor binding and Na(+), K(+)-ATPase activity were similar following reventilation with 21% or 100% oxygen after a single episode of mild asphyxia.
Hoffman
David Joseph
DJ
Department of Pediatrics, Drexel University College of Medicine, Philadelphia, PA, USA. hoffmandj@readinghospital.org
Lombardini
Eric
E
Mishra
Om Prakash
OP
Delivoria-Papadopoulos
Maria
M
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D001237:Asphyxia / Q000628:therapy; D001921:Brain / Q000166:cytology / Q000378:metabolism; D016291:Dizocilpine Maleate / Q000378:metabolism; D018691:Excitatory Amino Acid Antagonists / Q000378:metabolism; D006801:Humans; D007231:Infant, Newborn; D010100:Oxygen / Q000378:metabolism*; D010102:Oxygen Inhalation Therapy; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D012151:Resuscitation; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D018691:Excitatory Amino Acid Antagonists; D016194:Receptors, N-Methyl-D-Aspartate; D016291:Dizocilpine Maleate; D000255:Adenosine Triphosphate; D000254:Sodium-Potassium-Exchanging ATPase; D010100:Oxygen