Interspecies DNA microarray analysis identifies WblA as a pleiotropic down-regulator of antibiotic biosynthesis in Streptomyces.
Using Streptomyces coelicolor microarrays to discover regulators of gene expression in other Streptomyces species, we identified wblA, a whiB-like gene encoding a putative transcription factor, as a down-regulator of doxorubicin biosynthesis in Streptomyces peucetius. Further analysis revealed that wblA functions pleiotropically to control antibiotic production and morphological differentiation in streptomycetes. Our results reveal a novel biological role for wblA and show the utility of interspecies microarray analysis for the investigation of streptomycete gene expression.
Kang
Seung-Hoon
SH
Department of Biological Engineering, Inha University, Incheon, Korea.
Huang
Jianqiang
J
Lee
Han-Na
HN
Hur
Yoon-Ah
YA
Cohen
Stanley N
SN
Kim
Eung-Soo
ES
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004317:Doxorubicin / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*; D048372:Streptomyces coelicolor / Q000235:genetics / Q000378:metabolism
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004317:Doxorubicin