title: [Identification of novel genes related to glioma by oligonucleotide microarray].
abstract: OBJECTIVE
  To identify the genes differentially expressed in development of human glioma, and to study the expression of some genes in different grade gliomas.
  
  
  METHODS
  Oligonucleotide microarray (including 218 genes related to neural system development) was adopted and hybridized with probes which were prepared from the total RNAs of glioma specimens and normal brain tissues. Differentially expressed genes between the normal tissues and glioma tissues were assayed after scanning oligonuceltide microarray with ScanArray 4000, and some of these genes such as smad1, Hmp19 and TRIP3 were verified by real-time quantitative PCR(real-time-Q-PCR) method.
  
  
  RESULTS
  In comparison with the genes in the normal brain tissue, 5 down-regulated and 5 up-regulated genes in glioma specimens were revealed by means of microarrays, and the expression of smad1, Hmp19 and TRIP3 were verified by real-time-Q-PCR assay.
  
  
  CONCLUSIONS
  Multiple genes play important roles in development of glioma. cDNA microarray technology is a powerful technique in screening for differentially expressed genes between glioma tissues and normal brain tissues. This study is helpful for judgement of invasion and prognosis of gliomas, and provides more target genes for targeted therapy.
authors.affiliation: Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing, 100850 PR China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Liu
authors.forename: Zeng-min
authors.initials: ZM
authors.lastname: Tian
authors.forename: Guo-qiang
authors.initials: GQ
authors.lastname: Zheng
authors.forename: Hai-tao
authors.initials: HT
authors.lastname: Wu
authors.forename: Yan-bin
authors.initials: YB
authors.lastname: Jin
authors.forename: Xin
authors.initials: X
authors.lastname: Ma
authors.forename: Wen-hong
authors.initials: WH
authors.lastname: Fan
authors.forename: Ming
authors.initials: M
authors.lastname: Fan
mesh_terms: D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction