title: Development of a novel ELISA for detection of anti-A and anti-B antibodies in recipients of ABO-incompatible living donor liver grafts.
abstract: The survival rate in ABO-incompatible (ABO-I) liver transplantation was much lower than that in ABO-compatible recipients for the early experiences. It is therefore essential to develop the precise and fast measurement of anti-A and anti-B antibodies (Abs) to prevent humoral rejection in ABO-I liver transplantation. Agglutination titer has been the standard method to measure these Abs, but the interpretation of the results is subject to bias. Here, we have developed an objective and quantitative enzyme-linked immunosorbent assay (ELISA) to measure anti-A and anti-B Abs. This test requires only a small amount (10 microl) of recipient's serum. We applied the newly developed ELISA to monitor living donor liver transplant recipients and investigated the correlation between ELISA and agglutination titer. The Spearman's correlation coefficient for Abs ranged from 0.461 to 0.812. Moreover, in one case of humoral rejection, the increase of Abs was detected by ELISA one day earlier than by the agglutination titer. In conclusion, our ELISA method proved useful to detect an increase of anti-A and anti-B Abs titers at an early stage, thereby contributing to a prompt treatment of humoral rejection due to ABO-I.
authors.affiliation: Advanced Surgical Science and Technology, Tohoku University, Sendai, Japan.
authors.forename: Akefumi
authors.initials: A
authors.lastname: Satoh
authors.forename: Naoki
authors.initials: N
authors.lastname: Kawagishi
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Minegishi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Takahashi
authors.forename: Yorihiro
authors.initials: Y
authors.lastname: Akamatsu
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Doi
authors.forename: Susumu
authors.initials: S
authors.lastname: Satomi
mesh_terms: D000017:ABO Blood-Group System / Q000276:immunology; D000328:Adult; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006084:Graft Rejection / Q000276:immunology; D006386:Hemagglutination Tests; D006650:Histocompatibility Testing; D006801:Humans; D007223:Infant; D007518:Isoantibodies / Q000097:blood*; D016031:Liver Transplantation / Q000009:adverse effects / Q000276:immunology*; D019520:Living Donors; D008875:Middle Aged; D015996:Survival Rate
chemical_list: D000017:ABO Blood-Group System; D007518:Isoantibodies