title: Leptin inhibits hepatocyte growth factor-induced ductal morphogenesis of bovine mammary epithelial cells.
abstract: We examined the effect of stroma-derived factors, hepatocyte growth factor (HGF) and leptin, on morphological differentiation of bovine mammary epithelial cells (BMEC) in collagen gel three-dimensional culture in vitro. BMEC treated with HGF, but not leptin, formed duct-like organoids. The formation of organoids by HGF was enhanced by treatment with a mixture of insulin, cortisol and prolactin, while BMEC treated with the mixture alone did not produce the organoid. In contrast, the formation of organoids by HGF was dose-dependently inhibited by simultaneous addition of leptin, regardless of the presence or absence of the hormone mixture. These results suggest that stroma-derived factors intricately regulate mammary epithelial morphogenesis.
authors.affiliation: Laboratories of Biochemistry, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Yamaji
authors.forename: Akihiro
authors.initials: A
authors.lastname: Kamikawa
authors.forename: Mohamed M
authors.initials: MM
authors.lastname: Soliman
authors.forename: Tomoki
authors.initials: T
authors.lastname: Ito
authors.forename: Mohamed M
authors.initials: MM
authors.lastname: Ahmed
authors.forename: Kennedy
authors.initials: K
authors.lastname: Makondo
authors.forename: Atsushi
authors.initials: A
authors.lastname: Watanabe
authors.forename: Masayuki
authors.initials: M
authors.lastname: Saito
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kimura
mesh_terms: D000818:Animals; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects; D004347:Drug Interactions; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects; D005260:Female; D017228:Hepatocyte Growth Factor / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D066298:In Vitro Techniques; D020738:Leptin / Q000494:pharmacology*; D008321:Mammary Glands, Animal / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D009024:Morphogenesis / Q000187:drug effects / Q000502:physiology
chemical_list: D020738:Leptin; D017228:Hepatocyte Growth Factor