Mutant presenilin 2 increased oxidative stress and p53 expression in neuronal cells.
The learning and memory impairment of presenilin 2 transgenic mice was mentioned previously. In this study, exposing the presenilin 2 transfected PC12 cells to the 50 microM Abeta(25-35), 30 mM l-glutamate and 50 microM H(2)O(2) resulted in significant increase 8-oxodG and p53 levels of the cells expressing the mutant gene. The increase was also found in the mutant presenilin 2 transgenic mice brains age-dependently in comparison to that in the wild-type presenilin 2-transgenic mice and non-transgenic ones. These findings indicated that mutant presenilin 2 clearly increases oxidative stress and p53 expression, which could be implicated in promoting mutant presenilin 2-induced neurodegeneration in Alzheimer's disease, and the influence of mutant presenilin 2 in Alzheimer's disease may be brain regional and age related effects.
Nguyen
Hong Nga
HN
College of Pharmacy, CBITRC, Chungbuk National University, 12, Gaeshin-dong, Heungduk-gu, Cheongju, Chungbuk 361-763, Republic of Korea.
Lee
Moon Soon
MS
Hwang
Dae Youn
DY
Kim
Young Kyu
YK
Yoon
Do Young
DY
Lee
Jae Woong
JW
Yun
Yeo Pyo
YP
Lee
Myung Koo
MK
Oh
Ki Wan
KW
Hong
Jin Tae
JT
D000544:Alzheimer Disease / Q000378:metabolism*; D000818:Animals; D009154:Mutation; D009474:Neurons / Q000378:metabolism*; D018384:Oxidative Stress; D016716:PC12 Cells; D053766:Presenilin-2 / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
D053766:Presenilin-2; D016159:Tumor Suppressor Protein p53