Monoallelic expression of MAO-A in skin fibroblasts.
X chromosome inactivation in mammalian females occurs early in embryonic development and renders most genes on the inactive X chromosome transcriptionally silenced. As a consequence, females will display an X chromosomal parent-of-origin mosaicism with regard to which parental allele that is expressed. Some genes however, escape inactivation and will therefore be expressed from both alleles. In this study we have investigated if the X-linked MAO-A gene have bi- or mono-allelic expression. This information would indicate whether or not MAO-A gene dosage could potentially explain the observed gender differences that show functional connections to the serotonin system, such as aggression and impulsiveness. To investigate the X inactivation status of MAO-A we have used primary clonal cell cultures, on which allelic expression was assessed with RFLP analysis. Our results show that the MAO-A gene has mono-allelic expression in these cells. This could have important implications for understanding traits that display gender differences.
Nordquist
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Department of Neuroscience, Uppsala University, Uppsala, Sweden. niklas.nordquist@neuro.uu.se
Oreland
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D001923:Brain Chemistry / Q000235:genetics; D002478:Cells, Cultured; D002999:Clone Cells; D004303:Dosage Compensation, Genetic / Q000235:genetics*; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000201:enzymology; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D050172:Genes, X-Linked / Q000235:genetics; D006801:Humans; D008297:Male; D001523:Mental Disorders / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D008995:Monoamine Oxidase / Q000096:biosynthesis* / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D012701:Serotonin / Q000378:metabolism; D012727:Sex Characteristics; D012867:Skin / Q000166:cytology / Q000201:enzymology; D049951:X Chromosome Inactivation / Q000235:genetics*
D012701:Serotonin; D008995:Monoamine Oxidase