title: Cytotoxic withanolides from Tubocapsicum anomalum.
abstract: Fifteen new withanolides (1-8, 11-17) and two known withanolides, withanolide D (9) and 17alpha-hydroxywithanolide D (10), were isolated from the stems, roots, and leaves of Tubocapsicum anomalum using bioassay-directed fractionation. The structures were determined by spectroscopic and chemical methods, and the absolute configurations were established by CD analysis and by the Mosher ester method. The structure of 1 and 3 were further confirmed by X-ray crystallographic analysis. Compounds 1, 4-6, 8-10, and 13 showed significant cytotoxic activity against Hep G2, Hep 3B, A-549, MDA-MB-231, MCF-7, and MRC-5 cell lines.
authors.affiliation: Graduate Institute of Natural Products, School of Pharmcy, Kaohsiung Medical University, Kaohsiung 807, Taiwan, Republic of China.
authors.forename: Pei-Wen
authors.initials: PW
authors.lastname: Hsieh
authors.forename: Zih-You
authors.initials: ZY
authors.lastname: Huang
authors.forename: Jyun-Hong
authors.initials: JH
authors.lastname: Chen
authors.forename: Fang-Rong
authors.initials: FR
authors.lastname: Chang
authors.forename: Ching-Chung
authors.initials: CC
authors.lastname: Wu
authors.forename: Yu-Liang
authors.initials: YL
authors.lastname: Yang
authors.forename: Michael Y
authors.initials: MY
authors.lastname: Chiang
authors.forename: Ming-Hon
authors.initials: MH
authors.lastname: Yen
authors.forename: Shu-Li
authors.initials: SL
authors.lastname: Chen
authors.forename: Hsin-Fu
authors.initials: HF
authors.lastname: Yen
authors.forename: Tilo
authors.initials: T
authors.lastname: Lübken
authors.forename: Wen-Chun
authors.initials: WC
authors.lastname: Hung
authors.forename: Yang-Chang
authors.initials: YC
authors.lastname: Wu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018360:Crystallography, X-Ray; D004354:Drug Screening Assays, Antitumor; D004875:Ergosterol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D008968:Molecular Conformation; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D019657:Solanaceae / Q000737:chemistry*; D013624:Taiwan; D054358:Withanolides
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D054358:Withanolides; C010376:withanolide D; D004875:Ergosterol