title: Immune responses of pigs inoculated with a recombinant fowlpox virus coexpressing GP5/GP3 of porcine reproductive and respiratory syndrome virus and swine IL-18.
abstract: Two recombinant fowlpox viruses (rFPV-ORF5-ORF3 and rFPV-IL-18-ORF5-ORF3) containing the ORF5/ORF3 cDNAs of PRRSV (strain Chang Chun) and IL-18 of swine were constructed and evaluated for theirs abilities to induce humoral and cellular responses in piglets. In addition, their abilities to protect piglets against homologous virus challenge were examined. All piglets were given booster vaccinations at 21 days after the initial inoculation, and all piglets were challenged at 60 after the initial inoculation. Control groups were inoculated with wild-type fowlpox virus (wtFPV). All animals vaccinated with rFPV-ORF5-ORF3 and rFPV-IL-18-ORF5-ORF3 developed specific anti-PRRSV ELISA antibody and neutralizing antibody, as well as T-lymphocyte proliferation response. To evaluate the cellular immune function, IFN-gamma production in pigs serum and T-lymphocytes (CD4 and CD8 T cells) in peripheral blood were examined. Following challenge with a pathogenic strain of PRRSV (strain Chang Chun), piglets inoculated with recombinant fowlpox virus (rFPV) showed lower (P<0.05) temperature, viremia and virus load in bronchial lymph nodes than control animals, suggesting the establishment of partial protection against PRRSV infection. The results demonstrated the potential use of a fowlpox virus-based recombinant vaccine in the control and prevention of PRRSV infections.
authors.affiliation: Genetic Engineering Laboratory, Academy of Military Medical Sciences, Changchun 130062, PR China.
authors.forename: Guoshun
authors.initials: G
authors.lastname: Shen
authors.forename: Ningyi
authors.initials: N
authors.lastname: Jin
authors.forename: Mingxiao
authors.initials: M
authors.lastname: Ma
authors.forename: Kuoshi
authors.initials: K
authors.lastname: Jin
authors.forename: Min
authors.initials: M
authors.lastname: Zheng
authors.forename: Tianzhong
authors.initials: T
authors.lastname: Zhuang
authors.forename: Huijun
authors.initials: H
authors.lastname: Lu
authors.forename: Guangze
authors.initials: G
authors.lastname: Zhu
authors.forename: Hongtao
authors.initials: H
authors.lastname: Jin
authors.forename: Minglan
authors.initials: M
authors.lastname: Jin
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Huo
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Qin
authors.forename: Ronglan
authors.initials: R
authors.lastname: Yin
authors.forename: Chang
authors.initials: C
authors.lastname: Li
authors.forename: Hongwen
authors.initials: H
authors.lastname: Li
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Han
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Chen
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Jin
mesh_terms: D000276:Adjuvants, Immunologic / Q000235:genetics; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001831:Body Temperature; D049109:Cell Proliferation; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005587:Fowlpox virus / Q000235:genetics / Q000276:immunology*; D007117:Immunization, Secondary; D007371:Interferon-gamma / Q000096:biosynthesis / Q000097:blood; D020382:Interleukin-18 / Q000235:genetics / Q000276:immunology*; D008198:Lymph Nodes / Q000821:virology; D009500:Neutralization Tests; D019318:Porcine Reproductive and Respiratory Syndrome / Q000276:immunology* / Q000517:prevention & control*; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics / Q000276:immunology*; D013552:Swine; D013601:T-Lymphocytes / Q000276:immunology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*; D014766:Viremia
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D020382:Interleukin-18; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C114789:glycoprotein 5, PRRSV; D007371:Interferon-gamma