SIZ1-mediated sumoylation of ICE1 controls CBF3/DREB1A expression and freezing tolerance in Arabidopsis.
SIZ1 is a SUMO E3 ligase that facilitates conjugation of SUMO to protein substrates. siz1-2 and siz1-3 T-DNA insertion alleles that caused freezing and chilling sensitivities were complemented genetically by expressing SIZ1, indicating that the SIZ1 is a controller of low temperature adaptation in plants. Cold-induced expression of CBF/DREB1, particularly of CBF3/DREB1A, and of the regulon genes was repressed by siz1. siz1 did not affect expression of ICE1, which encodes a MYC transcription factor that is a controller of CBF3/DREB1A. A K393R substitution in ICE1 [ICE1(K393R)] blocked SIZ1-mediated sumoylation in vitro and in protoplasts identifying the K393 residue as the principal site of SUMO conjugation. SIZ1-dependent sumoylation of ICE1 in protoplasts was moderately induced by cold. Sumoylation of recombinant ICE1 reduced polyubiquitination of the protein in vitro. ICE1(K393R) expression in wild-type plants repressed cold-induced CBF3/DREB1A expression and increased freezing sensitivity. Furthermore, expression of ICE1(K393R) induced transcript accumulation of MYB15, which encodes a MYB transcription factor that is a negative regulator of CBF/DREB1. SIZ1-dependent sumoylation of ICE1 may activate and/or stabilize the protein, facilitating expression of CBF3/DREB1A and repression of MYB15, leading to low temperature tolerance.
Miura
Kenji
K
Center for Plant Environmental Stress Physiology, Purdue University, West Lafayette, Indiana 47907-2010, USA.
Jin
Jing Bo
JB
Lee
Jiyoung
J
Yoo
Chan Yul
CY
Stirm
Vicki
V
Miura
Tomoko
T
Ashworth
Edward N
EN
Bressan
Ray A
RA
Yun
Dae-Jin
DJ
Hasegawa
Paul M
PM
D017360:Arabidopsis / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D005615:Freezing; D008025:Ligases / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified / Q000378:metabolism; D011523:Protoplasts / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D025801:Ubiquitin / Q000378:metabolism
D029681:Arabidopsis Proteins; C114578:DREB1A protein, Arabidopsis; C510502:ICE1 protein, Arabidopsis; D011994:Recombinant Proteins; D014157:Transcription Factors; D025801:Ubiquitin; D008025:Ligases; C502552:SIZ1 protein, Arabidopsis