title: Stress and high heart rate provoke ventricular tachycardia in mice expressing triadin.
abstract: Reduced function of the cardiac ryanodine receptor or calsequestrin causes catecholaminergic ventricular tachycardia (VT). These proteins regulate sarcoplasmic Ca(2+) release in close conjunction with two accessory proteins, triadin and junctin. Based on data from cardiomyocytes, we hypothesized that enhanced triadin expression could cause VT. We assessed arrhythmias and electrophysiological changes in vivo and in the beating heart in mice expressing junctin, triadin, or both proteins (TRDxJCN), and measured calcium transients in isolated ventricular cardiomyocytes. TRDxJCN mice were studied to compensate the down-regulation of junctin expression in triadin-expressing mice. Exercise or stress provoked repetitive VT in freely roaming TRDxJCN mice whenever heart rate increased above approximately 600 bpm (p<0.05 vs. the three other genotypes). TRDxJCN mice expressed total triadin 2.9-fold (p<0.05) and total junctin not different to wildtype (p=ns). Left ventricular systolic function was not different between lineages. beta-adrenoreceptor stimulation (orciprenaline 1.7 microM) provoked early-coupled ventricular ectopy and repetitive VT in isolated, Langendorff-perfused TRDxJCN hearts (p<0.05). Under conditions associated with VT (high pacing rate, catecholamine stimulation), action potential duration was shorter in TRDxJCN with VT than in the other genotypes and shorter than in TRDxJCN hearts without VT (p<0.05). Ca(2+) transient duration was prolonged in Indo1-loaded TRDxJCN cardiomyocytes under VT-provoking conditions. Action potential prolongation by mexiletine (2 microM or 4 microM) or clarithromycine (150 microM) suppressed VT. Expression of triadin provokes stress- and tachycardia-related ventricular arrhythmias in mice. An imbalance between prolonged intracellular calcium release and shortening of the ventricular action potential may contribute to genesis of arrhythmias in this model.
authors.affiliation: Department of Cardiology and Angiology, Hospital of the University of Münster, Germany. kirchhp@uni-muenster.de
authors.forename: Paulus
authors.initials: P
authors.lastname: Kirchhof
authors.forename: Jan
authors.initials: J
authors.lastname: Klimas
authors.forename: Larissa
authors.initials: L
authors.lastname: Fabritz
authors.forename: Melanie
authors.initials: M
authors.lastname: Zwiener
authors.forename: Larry R
authors.initials: LR
authors.lastname: Jones
authors.forename: Michael
authors.initials: M
authors.lastname: Schäfers
authors.forename: Sven
authors.initials: S
authors.lastname: Hermann
authors.forename: Peter
authors.initials: P
authors.lastname: Boknik
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Schmitz
authors.forename: Günter
authors.initials: G
authors.lastname: Breithardt
authors.forename: Uwe
authors.initials: U
authors.lastname: Kirchhefer
authors.forename: Joachim
authors.initials: J
authors.lastname: Neumann
mesh_terms: D000200:Action Potentials; D000818:Animals; D002135:Calcium-Binding Proteins / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics*; D003433:Crosses, Genetic; D005260:Female; D006321:Heart / Q000502:physiology*; D006339:Heart Rate; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D006899:Mixed Function Oxygenases / Q000235:genetics; D009124:Muscle Proteins / Q000235:genetics*; D005082:Physical Exertion; D017180:Tachycardia, Ventricular / Q000503:physiopathology*
chemical_list: D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009124:Muscle Proteins; C498270:Trdn protein, mouse; C498841:Asph protein, mouse; D006899:Mixed Function Oxygenases