Association of serum cytokine concentrations with psychological symptoms in midlife women.
OBJECTIVE
The purpose of the present study was to clarify the association of serum cytokine concentrations, determined using a multiplexed cytokine assay, with psychological symptoms in midlife women.


METHODS
Fifty-three peri- and post-menopausal women with and without psychological symptoms in Greene's climacteric scale were enrolled in this study. Levels of 17 cytokines in serum samples were measured simultaneously using a multiplexed human cytokine assay.


RESULTS
Serum interleukin (IL)-6 concentration in women with psychological symptoms (2.71+/-047 pg/ml) was significantly (p=0.009) higher than that in women without psychological symptoms (0.98+/-0.18 pg/ml). Serum IL-8 concentration in women with psychological symptoms (33.4+/-8.17 pg/ml) was also significantly (p=0.022) higher than that in women without psychological symptoms (7.87+/-1.64 pg/ml). In addition, serum IL-10 concentration in women with psychological symptoms (0.74+/-0.26 pg/ml) was significantly (p=0.048) higher than that in women without psychological symptoms (0.07+/-0.04 pg/ml). Tumor necrosis factor (TNF)-alpha in serum was detected only in women with psychological symptoms. Serum IL-2 concentration in women with psychological symptoms tended (p=0.066) to be higher than that in women without psychological symptoms. No significant differences were found between levels of other cytokines in women with and without psychological symptoms.


CONCLUSIONS
Psychological stress manifested as climacteric symptoms in midlife women may be associated with increases in serum concentrations of IL-6, IL-8, IL-10, and TNF-alpha.
Yasui
Toshiyuki
T
Department of Obstetrics and Gynecology, Course of Human Development, Human Development and Health Science, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima, Japan. yasui@clin.med.tokushima-u.ac.jp
Maegawa
Masahiko
M
Tomita
Junko
J
Miyatani
Yuka
Y
Yamada
Masayo
M
Uemura
Hirokazu
H
Ueno
Shu-ichi
S
Numata
Shusuke
S
Ohmori
Tetsuro
T
Tsuchiya
Naoko
N
Yuzurihara
Mitsutoshi
M
Takeda
Shuichi
S
Irahara
Minoru
M
D016207:Cytokines / Q000097:blood*; D005260:Female; D006801:Humans; D008593:Menopause / Q000097:blood / Q000276:immunology* / Q000523:psychology*; D008875:Middle Aged; D047648:Perimenopause / Q000097:blood / Q000276:immunology / Q000523:psychology; D017698:Postmenopause / Q000097:blood / Q000276:immunology / Q000523:psychology; D013315:Stress, Psychological / Q000097:blood / Q000276:immunology*
D016207:Cytokines