title: Solubilization and characterization of a cell wall-bound trehalase from ascospores of the fission yeast Schizosaccharomyces pombe.
abstract: The genome of the fission yeast Schizosaccharomyces pombe lacks sequence homologs to ath1 genes coding for acid trehalases in other yeasts or filamentous fungi. However, acid trehalase activity is present at the spore stage in the life cycle of the fission yeast. The enzyme responsible for this activity behaves as a surface enzyme covalently linked to the spore cell walls in both wild-type and ntp1 mutant strains devoid of neutral trehalase. Lytic treatment of particulated cell wall fractions allowed the solubilization of the enzyme into an active form. We have characterized this soluble enzyme and found that its kinetic parameters, optimum pH and temperature, thermal denaturation and salt responses are closely similar to other conventional acid trehalases. Hence, this rather unusual enzyme can be recognized as acid trehalase by its biochemical properties although it does not share genetic homology with other known acid trehalases. The potential role of such acid trehalase in the mobilization of trehalose is discussed.
authors.affiliation: Department of Genetics and Microbiology, Facultad de Biología, University of Murcia, Murcia, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Vicente-Soler
authors.forename: T
authors.initials: T
authors.lastname: Soto
authors.forename: M
authors.initials: M
authors.lastname: Madrid
authors.forename: A
authors.initials: A
authors.lastname: Núñez
authors.forename: J
authors.initials: J
authors.lastname: Cansado
authors.forename: M
authors.initials: M
authors.lastname: Gacto
mesh_terms: D002458:Cell Fractionation; D002473:Cell Wall / Q000201:enzymology*; D020536:Enzyme Activators / Q000494:pharmacology; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011489:Protein Denaturation; D012492:Salts / Q000494:pharmacology; D012568:Schizosaccharomyces / Q000201:enzymology*; D013172:Spores, Fungal / Q000201:enzymology*; D013696:Temperature; D014198:Trehalase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D020536:Enzyme Activators; D005656:Fungal Proteins; D012492:Salts; D014198:Trehalase