Enrichment methodology to increase the positivity of cultures from body fluids.
Isolation and identification of etiological agents found in body fluids can be of critical importance for the recovery of patients suffering from potentially-severe infections, which are often followed by serious sequels. Eighty-two samples of different body fluids were analyzed using two different methods: (1) the conventional culture method (agar plating) and (2) the enrichment culture technique, using the Bact/Alert blood culture bottle. The number of positive cultures increased on average from 9.7% to 23.1% with the enrichment culture technique. Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus were the most frequently isolated bacteria. The enrichment method could provide a more accurate means the identifying etiological agents.
Daur
Alessandra Valle
AV
Clinical Laboratory, Curitiba General Hospital, Curitiba, PR, Brazil.
Klimak
Francisco
F
Cogo
Laura Lúcia
LL
Botão
Gislene Diógenes
GD
Monteiro
Cristina Leise Bastos
CL
Dalla Costa
Libera Maria
LM
D015373:Bacterial Typing Techniques / Q000379:methods*; D001826:Body Fluids / Q000382:microbiology*; D003470:Culture Media; D006090:Gram-Negative Bacteria / Q000302:isolation & purification*; D006094:Gram-Positive Bacteria / Q000302:isolation & purification*; D006801:Humans
D003470:Culture Media