[Study on inducing differentiation of mouse embryonic stem cells into cardiomyocyte in vitro].
OBJECTIVE
To study the differentiation from embeyonic stem cells into cardiomyocyte.


METHODS
Gene alpha/ beta-MHC, a specific gene of cardiomyocyte and another three house-keeping genes were detected by RT-PCR from the ESCs of 0-,2-,4-,6-,8-, 10-,12-,14-,16-,18- and 20 day and ESCs of 0-,3-, 5- and 10 day with specific culture condition.


RESULTS
ESCs started to express alpha/betaMHC gene from 6 day and the levels of alpha/betaMHC increased with culture time. The levels of 14 day's alpha/betaMHC were the highest. After 14 day, alpha/betaMHC gene kept the stable levels. The levels of three house-keeping genes changed significantly with culture time, and GAPDH kept the best stability of three genes.


CONCLUSIONS
The cycle of the differentiation from ESCs into cardiomyocyte in vitro could be designed as 10 - 12 days and GAPDH could be selected as the fittest inner comparison.
Yu
Zhou
Z
Institute of Nutrition and Food Safety, Chinese CDC, Beijing 100021, China.
Xu
Haibin
H
Yan
Weixing
W
Zhang
Lianfeng
L
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology*; D005260:Female; D020869:Gene Expression Profiling; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D032383:Myocytes, Cardiac / Q000166:cytology*; D011247:Pregnancy
D005987:Glyceraldehyde-3-Phosphate Dehydrogenases