Chlamydomonas reinhardtii CNX1E reconstitutes molybdenum cofactor biosynthesis in Escherichia coli mutants.
We have isolated and characterized the Chlamydomonas reinhardtii genes for molybdenum cofactor biosynthesis, namely, CNX1G and CNX1E, and expressed them and their chimeric fusions in Chlamydomonas and Escherichia coli. In all cases, the wild-type phenotype was restored in individual mutants as well as in a CNX1G CNX1E double mutant. Therefore, CrCNX1E is the first eukaryotic protein able to complement an E. coli moeA mutant.
Llamas
Angel
A
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Córdoba, Campus de Rabanales, Edificio Severo Ochoa, 14071 Córdoba, Spain.
Tejada-Jimenez
Manuel
M
González-Ballester
David
D
Higuera
José Javier
JJ
Schwarz
Guenter
G
Galván
Aurora
A
Fernández
Emilio
E
D020418:Algal Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000235:genetics / Q000378:metabolism*; D003067:Coenzymes / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005816:Genetic Complementation Test; D008667:Metalloproteins / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D000090006:Molybdenum Cofactors; D009687:Nuclear Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D015800:Protozoan Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D011621:Pteridines; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
D020418:Algal Proteins; D003067:Coenzymes; D008667:Metalloproteins; D000090006:Molybdenum Cofactors; D009687:Nuclear Proteins; D015800:Protozoan Proteins; D011621:Pteridines; D011993:Recombinant Fusion Proteins; C022261:molybdopterin