Using tRNA-linked molecular beacons to image cytoplasmic mRNAs in live cells.
Imaging products of gene expression in live cells will provide unique insights into the biology of cells. Molecular beacons make attractive probes for imaging mRNA in live cells as they can report the presence of an RNA target by turning on the fluorescence of a quenched fluorophore. However, when oligonucleotide probes are introduced into cells, they are rapidly sequestered in the nucleus, making the detection of cytoplasmic mRNAs difficult. We have shown that if a molecular beacon is linked to a tRNA, it stays in the cytoplasm and permits detection of cytoplasmic mRNAs. Here we describe two methods of linking molecular beacons to tRNA and show how the joint molecules can be used for imaging an mRNA that is normally present in the cytoplasm in live cultured cells. This protocol should take a total of 4 d to complete.
Mhlanga
Musa M
MM
Public Health Research Institute, 225 Warren Street, Newark, New Jersey 07103, USA.
Tyagi
Sanjay
S
D003593:Cytoplasm / Q000235:genetics*; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000378:metabolism; D015870:Gene Expression; D015336:Molecular Probe Techniques; D012333:RNA, Messenger / Q000235:genetics*; D012343:RNA, Transfer / Q000378:metabolism
D005456:Fluorescent Dyes; D012333:RNA, Messenger; D012343:RNA, Transfer