[The role of Cd-binding proteins and phytochelatins in the formation of cadmium resistance in Nicotiana plumbaginifolia cell lines].
Nicotiana plumbaginifolia callus lines with the equal resistance to cadmium have been produced under different selective conditions--either without inhibition of the phytochelatin synthesis (line Cd-R) or in the presence of the inhibitor butionine sulfoximine (line Cd-Ri). The level of phytochelatin synthesis in the line Cd-R five-fold exceeded the control value and in the line Cd-Ri it was twice as much as in the control. It was shown that in the control line mainly three cadmium-binding proteins are expressed of the molecular weihgts 41, 34 and 19 kD. The common feature of the both resistant lines is the expression of the cadmium-binding proteins of 40, 37 and 19 kD. The resistant lines differ with respect to the synthesis of relatively low-molecular cadmium-binding proteins. The proteins of the molecular weights 12.5, 11.5 and 9 kD are expressed in the line Cd-R, while the proteins of 13 and 10 kD are expressed in the line Cd-Ri. It was supposed that both the phytochelatins and the Cd-binding proteins contribute to the resisitance of N. plumbaginifolia callus lines to cadmium and the lack of the phytochelatins can be equilibrated by the changes in the low-molecular Cd-binding protein synthesis.
Fenik
S I
SI
Solodushko
V G
VG
Kaliniak
T B
TB
Blium
Ia B
IaB
D019256:Cadmium Chloride / Q000494:pharmacology*; D004351:Drug Resistance; D005978:Glutathione / Q000096:biosynthesis*; D007124:Immunoenzyme Techniques; D008668:Metallothionein / Q000096:biosynthesis*; D054811:Phytochelatins; D012641:Selection, Genetic; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
C004203:cadmium-binding protein; D008668:Metallothionein; D054811:Phytochelatins; D005978:Glutathione; D019256:Cadmium Chloride