title: A tetra(L-lysine)-grafted poly(organophosphazene) for gene delivery.
abstract: In order to develop a new gene delivery vector, a novel cationic poly(organophosphazene) was synthesized by stepwise nucleophilic substitutions of poly(dichlorophosphazene) with a hydrophilic methoxy-poly(ethylene glycol) (MPEG) as a shielding group and a branched tetra(L-lysine), LysLys(LysEt)(2), as a cationic moiety. The cationic polymer has shown to form a polyplex by DNA condensation and very low in vitro cytotoxicity probably due to the shielding effect of MPEG, which provides a basis for improving the low gene transfection yield of cationic polyphosphazenes.
authors.affiliation: Division of Nano Science BK 21, Ewha Womans University, 11-1 Daehyun-Dong, Seodaemun-Gu, Seoul 120-750, Republic of Korea.
authors.forename: Yong Joo
authors.initials: YJ
authors.lastname: Jun
authors.forename: Jung Hee
authors.initials: JH
authors.lastname: Kim
authors.forename: Su Jin
authors.initials: SJ
authors.lastname: Choi
authors.forename: Hwa Jeong
authors.initials: HJ
authors.lastname: Lee
authors.forename: Moo Jin
authors.initials: MJ
authors.lastname: Jun
authors.forename: Youn Soo
authors.initials: YS
authors.lastname: Sohn
mesh_terms: D045744:Cell Line, Tumor; D004247:DNA / Q000737:chemistry; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000737:chemistry*; D006801:Humans; D008156:Luciferases / Q000032:analysis / Q000235:genetics; D009943:Organophosphorus Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D011107:Polylysine / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D014162:Transfection
chemical_list: C521492:MPEG350; D009943:Organophosphorus Compounds; D011108:Polymers; C108974:poly(phosphazene); D011107:Polylysine; D011092:Polyethylene Glycols; D004247:DNA; C033952:tetralysine; D008156:Luciferases