Evidence for new beta1-3 galactosyltransferase activity involved in biosynthesis of unusual N-glycan harboring T-antigen in Apis mellifera.
In a previous study (Y. Kimura et al., Biosci. Biotechnol. Biochem., 70, 2583-2587, 2006), we found that new complex type N-glycans harboring Thomsen-Friedenreich antigen (Galbeta1-3GalNAc) unit occur on royal jelly glycoproteins, suggesting the involvement of a new beta1-3galactosyltransferase in the synthesis of the unusual complex type N-glycans. So far, such beta1-3galactosyltransferase activity, which can transfer galactosyl residues with the beta1-3 linkage to beta1-4 GalNAc residues in N-glycan, has not been found among any eucaryotic cells. But using GalNAc(2)GlcNAc(2)Man(3)GlcNAc(2)-PA as acceptor N-glycan, we detected the beta1-3 galactosyltransferase activity in membrane fraction prepared from honeybee cephalic portions. This result indicates that honeybee expresses a unique beta1-3 galactosyltransferase involved in biosynthesis of the unusual N-glycan containing a tumor related antigen in the hypopharyngeal gland.
Kimura
Yoshinobu
Y
Department of Biofunctional Chemistry, Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan. yosh8mar@cc.okayama-u.ac.jp
Sakamura
Sho
S
Ushijima
Takayuki
T
Hama
Yoichiro
Y
Kajiura
Hiroyuki
H
Fujiyama
Kazuhito
K
Okihara
Kiyoshi
K
Hashimoto
Ken
K
Sugimoto
Hiroyuki
H
Yamada
Hideo
H
D000818:Animals; D000957:Antigens, Viral, Tumor / Q000276:immunology*; D001516:Bees / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D030541:Databases, Genetic; D004926:Escherichia coli / Q000378:metabolism; D005700:Galactosyltransferases / Q000235:genetics* / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D008861:Microsomes / Q000201:enzymology; D011134:Polysaccharides / Q000096:biosynthesis* / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization
D000957:Antigens, Viral, Tumor; D011134:Polysaccharides; D011994:Recombinant Proteins; D005700:Galactosyltransferases