title: Vaccination with a T-cell-priming Gag peptide of caprine arthritis encephalitis virus enhances virus replication transiently in vivo.
abstract: CD4+ T cells are involved in several immune response pathways used to control viral infections. In this study, a group of genetically defined goats was immunized with a synthetic peptide known to encompass an immunodominant helper T-cell epitope of caprine arthritis encephalitis virus (CAEV). Fifty-five days after challenge with the molecularly cloned CAEV strain CO, the vaccinated animals had a higher proviral load than the controls. The measurement of gamma interferon and interleukin-4 gene expression showed that these cytokines were reliable markers of an ongoing immune response but their balance did not account for more or less efficient control of CAEV replication. In contrast, granulocyte-macrophage colony-stimulating factor appeared to be a key cytokine that might support virus replication in the early phase of infection. The observation of a potential T-cell-mediated enhancement of virus replication supports other recent findings showing that lentivirus-specific T cells can be detrimental to the host, suggesting caution in designing vaccine candidates.
authors.affiliation: Institute of Veterinary Virology, University of Bern, Switzerland.
authors.forename: Chiara
authors.initials: C
authors.lastname: Nenci
authors.affiliation: Institute of Veterinary Virology, University of Bern, Switzerland.
authors.forename: Marie-Luise
authors.initials: ML
authors.lastname: Zahno
authors.affiliation: Institute of Veterinary Virology, University of Bern, Switzerland.
authors.forename: Hans-Rudolf
authors.initials: HR
authors.lastname: Vogt
authors.affiliation: Institute of Animal Genetics, Nutrition and Housing, University of Bern, Switzerland.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Obexer-Ruff
authors.affiliation: Clinical Research, Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern, Switzerland.
authors.forename: Marcus G
authors.initials: MG
authors.lastname: Doherr
authors.affiliation: Institute of Veterinary Virology, University of Bern, Switzerland.
authors.forename: Reto
authors.initials: R
authors.lastname: Zanoni
authors.affiliation: Institute of Veterinary Virology, University of Bern, Switzerland.
authors.forename: Ernst
authors.initials: E
authors.lastname: Peterhans
authors.affiliation: Institute of Veterinary Virology, University of Bern, Switzerland.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Bertoni
mesh_terms: D000818:Animals; D001168:Arthritis / Q000662:veterinary / Q000821:virology; D016088:Arthritis-Encephalitis Virus, Caprine / Q000235:genetics* / Q000276:immunology*; D015683:Gene Products, gag / Q000276:immunology*; D015511:Goat Diseases / Q000821:virology; D006041:Goats; D010455:Peptides / Q000276:immunology*; D011014:Pneumonia / Q000662:veterinary / Q000821:virology; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology* / Q000821:virology; D014765:Viral Vaccines
chemical_list: D015683:Gene Products, gag; D010455:Peptides; D012333:RNA, Messenger; D012367:RNA, Viral; D014765:Viral Vaccines