title: Improved 16S rRNA-targeted probe set for analysis of sulfate-reducing bacteria by fluorescence in situ hybridization.
abstract: An updated dataset of in silico specificities for 54 previously published 16S rRNA-targeted oligonucleotides was assembled to provide guidance for reliable fluorescence in situ hybridization (FISH) analysis of sulfate-reducing bacteria. Additionally, six new FISH probes were developed for major deltaproteobacterial taxa, including a probe trio targeting most Deltaproteobacteria and Gemmatimonadetes.
authors.affiliation: Department of Microbial Ecology, Faculty of Life Sciences, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Lücker
authors.forename: Doris
authors.initials: D
authors.lastname: Steger
authors.forename: Kasper Urup
authors.initials: KU
authors.lastname: Kjeldsen
authors.forename: Barbara J
authors.initials: BJ
authors.lastname: MacGregor
authors.forename: Michael
authors.initials: M
authors.lastname: Wagner
authors.forename: Alexander
authors.initials: A
authors.lastname: Loy
mesh_terms: D030561:Databases, Nucleic Acid; D020564:Deltaproteobacteria / Q000235:genetics / Q000302:isolation & purification; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D015345:Oligonucleotide Probes; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013431:Sulfates / Q000378:metabolism*; D016999:Sulfur-Reducing Bacteria / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D015345:Oligonucleotide Probes; D012336:RNA, Ribosomal, 16S; D013431:Sulfates