title: Hemagglutinin pseudotyped lentiviral particles: characterization of a new method for avian H5N1 influenza sero-diagnosis.
abstract: BACKGROUND
  Highly pathogenic avian influenza (HPAI) H5N1 has spread globally in birds and infected over 270 humans with an apparently high mortality rate. Serologic studies to determine the extent of asymptomatic H5N1 infection in humans and other mammals and to investigate the immunogenicity of current H5N1 vaccine candidates have been hampered by the biosafety requirements needed for H5N1 micro-neutralization tests.
  
  
  OBJECTIVE
  Development of a serodiagnostic tool for highly pathogenic influenza that reproduces H5N1 biology but can be used with less biohazard.
  
  
  METHODS
  We have generated and evaluated H5 hemagglutinin pseudotyped lentiviral particles encoding the luciferase reporter (H5pp).
  
  
  RESULTS
  H5pp entry into target cells depends on alpha2-3 cell surface sialic acids and requires low pH for membrane fusion. H5pp infectivity is specifically neutralized by sera from patients and animals infected with H5N1 and correlates well with conventional microneutralization test.
  
  
  CONCLUSIONS
  H5pp reproduce H5N1 influenza virus entry into target cells and potentially provides a high-throughput and safe method for sero-epidemiology.
authors.affiliation: HKU-Pasteur Research Center, Hong Kong, SAR China. isabelle.nefkens@gmail.com
authors.forename: Isabelle
authors.initials: I
authors.lastname: Nefkens
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Garcia
authors.forename: Chu Shui
authors.initials: CS
authors.lastname: Ling
authors.forename: Nadège
authors.initials: N
authors.lastname: Lagarde
authors.forename: John
authors.initials: J
authors.lastname: Nicholls
authors.forename: Dong Jiang
authors.initials: DJ
authors.lastname: Tang
authors.forename: Malik
authors.initials: M
authors.lastname: Peiris
authors.forename: Philippe
authors.initials: P
authors.lastname: Buchy
authors.forename: Ralf
authors.initials: R
authors.lastname: Altmeyer
mesh_terms: D000818:Animals; D001717:Birds; D004285:Dogs; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D005585:Influenza in Birds / Q000097:blood / Q000175:diagnosis* / Q000821:virology*; D007251:Influenza, Human / Q000097:blood / Q000175:diagnosis* / Q000821:virology*; D016086:Lentivirus / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D008156:Luciferases / Q000235:genetics; D009500:Neutralization Tests / Q000379:methods; D012698:Serologic Tests / Q000379:methods; D014771:Virion / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; C109963:hemagglutinin, avian influenza A virus; D008156:Luciferases