The two-component signal system in rice (Oryza sativa L.): a genome-wide study of cytokinin signal perception and transduction.
In this report we define the genes of two-component regulatory systems in rice through a comprehensive computational analysis of rice (Oryza sativa L.) genome sequence databases. Thirty-seven genes were identified, including 5 HKs (cytokinin-response histidine protein kinase) (OsHK1-4, OsHKL1), 5 HPs (histidine phosphotransfer proteins) (OsHP1-5), 15 type-A RRs (response regulators) (OsRR1-15), 7 type B RR genes (OsRR16-22), and 5 predicted pseudo-response regulators (OsPRR1-5). Protein motif organization, gene structure, phylogenetic analysis, chromosomal location, and comparative analysis between rice, maize, and Arabidopsis are described. Full-length cDNA clones of each gene were isolated from rice. Heterologous expression of each of the OsHKs in yeast mutants conferred histidine kinase function in a cytokinin-dependent manner. Nonconserved regions of individual cDNAs were used as probes in expression profiling experiments. This work provides a foundation for future functional dissection of the rice cytokinin two-component signaling pathway.
Du
Liming
L
The Key State Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zijingang Campus, Hangzhou 310058, People's Republic of China.
Jiao
Fangchan
F
Chu
Jun
J
Jin
Gulei
G
Chen
Ming
M
Wu
Ping
P
D003583:Cytokinins / Q000378:metabolism*; D020869:Gene Expression Profiling; D017343:Genes, Plant; D018745:Genome, Plant; D000071677:Histidine Kinase; D012275:Oryza / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D015398:Signal Transduction
D003583:Cytokinins; D010940:Plant Proteins; D011994:Recombinant Proteins; D011494:Protein Kinases; D000071677:Histidine Kinase