Isolation, growth, and purification of defective adenovirus deletion mutants.
Defective adenovirus deletion mutants can be grown by complementation in the presence of helper viruses that supply essential functions missing in the deletion mutant. In general, the deletion mutant then must be separated physically from the helper for use in subsequent experiments. This chapter includes suggestions for selection of helper viruses, protocols for the production of stocks by complementation, and procedures for physical separation of deletion mutants from their helpers.
Ketner
Gary
G
Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, USA.
Boyer
Julie
J
D000256:Adenoviridae / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification*; D003673:Defective Viruses / Q000235:genetics* / Q000302:isolation & purification; D005816:Genetic Complementation Test; D006378:Helper Viruses / Q000235:genetics / Q000302:isolation & purification; D009154:Mutation; D017384:Sequence Deletion