[Preparation and characterization of human colon tumor-associated antigen and its clinical significance].
OBJECTIVE
To purify the colon tumor-associated antigen from cultured colon tumor cells, and to investigate its expression in the sera of patients with colon cancer.


METHODS
The monoclonal antibody (mAb) against human colon tumor-associated antigen 4D10 was employed as the ligand for the immunoaffinity chromatography to purify the colon tumor-associated antigen from the lysate of colorectal tumor cell LOVO. The purified antigen was identified by SDS-PAGE and Western blot. The expression of colon tumor-associated antigen in the sera of patients with colon cancer and in normal sera was detected by Sandwich ELISA.


RESULTS
The purified colon tumor-associated antigen binding to mAb 4D10 was a heterodimer composed of two subunits with relative molecular mass M(r) of 30 x 10(3) and 35 x 10(3) respectively. The antigen was significantly higher expressed in sera from patients with colon cancer than that in normal sera (P<0.01).


CONCLUSIONS
The tumor-associated antigen obtained from the colon tumor cells has been successfully purified through immunoaffinity chromatography with mAb 4D10, which may be useful for diagnosis on clinic.
Liu
Xiao-Yan
XY
College of Life Science, Anhui University, Hefei 230039, China.
Yao
Xiao-Ling
XL
Song
Yu
Y
Liu
Xiao-Yan
XY
Zhang
Ping
P
Xu
Zhen-Shan
ZS
Song
Li-Hua
LH
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000951:Antigens, Neoplasm / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002846:Chromatography, Affinity; D003110:Colonic Neoplasms / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008970:Molecular Weight; D011817:Rabbits
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm