[Expression of PCNA and GST-pi after stereotactic irradiation for liver cirrhosis and hepatocarcinoma in rabbits].
OBJECTIVE
To observe the changes of the cytokines following stereotactic irradiation for hepatocarcinoma with cirrhosis in rabbits.


METHODS
Sixteen rabbits with liver cirrhosis and hepatocarcinoma (experimental group) were randomized into two equal groups to receive stereotactic irradiation at single dose of 20 and 30 Gy, respectively. Eight rabbits with hepatocarcinoma (control group) were divided into two equal groups and treated in identical manner. All the rabbits were killed 3 weeks after irradiation, and EV two-step method was used to observe the cytokine changes of proliferating cell nuclear antigen (PCNA) and glutathione S-transferase pi (GST-pi) after irradiation.


RESULTS
After irradiation, PCNA and GST-pi expression showed significant difference in the adjacent liver tissue between the experimental and control rabbits with irradiation at 20 Gy (P=0.010), but not with the irradiation dose of 30 Gy (P=1.000). Irradiation at different doses resulted in significant difference in the cytokine expression in the experimental rabbits (P=0.010). In the liver tissue exposed to irradiation, different irradiation doses resulted in significant difference in PCNA and GST-pi protein expression (P=0.010).


CONCLUSIONS
For hepatocarcinoma with cirrhosis in rabbits, radiation at the single dose of 30 Gy produces better response than 20 Gy, and PCNA and GST-pi may serve as good indexes for evaluating the therapeutic effect.
Ding
Yi
Y
Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Chen
Long-hua
LH
Wu
De-hua
DH
Sun
Ai-min
AM
D000818:Animals; D051549:Glutathione S-Transferase pi / Q000096:biosynthesis*; D007150:Immunohistochemistry; D008106:Liver Cirrhosis, Experimental / Q000378:metabolism* / Q000532:radiotherapy; D008114:Liver Neoplasms, Experimental / Q000378:metabolism* / Q000532:radiotherapy; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis*; D011817:Rabbits; D011880:Radiotherapy Planning, Computer-Assisted / Q000379:methods; D020266:Radiotherapy, Conformal; D011897:Random Allocation
D018809:Proliferating Cell Nuclear Antigen; D051549:Glutathione S-Transferase pi