Signs of proinflammatory/genotoxic switch (adipogenotoxicosis) in mammary fat of breast cancer patients: role of menopausal status, estrogens and hyperglycemia.
The abundance of fat tissue surrounding normal and malignant epithelial mammary cells raises the questions whether such "adipose milieu" is important in the local proinflammatory/genotoxic shift, which apparently promotes tumor development and worsens prognosis, and what conditions stimulate this shift, or "adipogenotoxicosis." We studied 95 mammary fat samples from 70 postmenopausal and 25 premenopausal breast cancer (BC) patients at a distance of 1.5-2.0 cm from tumors. The levels of leptin, adiponectin, TNFalpha and IL-6 release after 4-hr incubation of the samples were evaluated with ELISA, nitric oxide (NO) production by Griess reaction and lipid peroxidation by determination of thiobarbiturate-reactive products (TBRP). Infiltration of fat with macrophages (CD68-positive cells) and expression of cytochrome P450 1B1/estrogen 4-hydroxylase (CYP1B1) were detected by immunohistochemistry. Aromatase (CYP19) activity in mammary fat was measured by (3)H(2)O release from (3)H-1beta-androstenedione. In the postmenopausal BC patients, NO and TNFalpha production by adipose tissue explants increased independent of BMI and in parallel with decreasing leptin and, especially, adiponectin release. In the premenopausal patients, higher CYP1B1 expression and TBRP level were found in mammary fat, while higher aromatase activity was combined with higher CYP1B1 expression as well as NO and IL-6 production. In the postmenopausal group, impaired glucose tolerance was associated with higher IL-6 release production by fat and with higher IL-6/adiponectin ratio. Thus, signs of adipogenotoxicosis in mammary fat can be found in both pre- and postmenopausal BC patients. This condition is likely being maintained through estrogen- and glucose-related factors and mechanisms presumably associated with less favorable types of hormonal carcinogenesis.
Berstein
Lev M
LM
Laboratory of Oncoendocrinology, N.N. Petrov Research Institute of Oncology, St. Petersburg, Russia. levmb@endocrin.spb.ru
Kovalevskij
Anatolij Y
AY
Poroshina
Tatjana E
TE
Kotov
Alexander V
AV
Kovalenko
Irina G
IG
Tsyrlina
Evgenia V
EV
Leenman
Elena E
EE
Revskoy
Sergey Y
SY
Semiglazov
Vladimir F
VF
Pozharisski
Kazimir M
KM
D000273:Adipose Tissue / Q000502:physiology*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001141:Aromatase / Q000378:metabolism; D001943:Breast Neoplasms / Q000473:pathology*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004967:Estrogens / Q000502:physiology*; D005260:Female; D006801:Humans; D006943:Hyperglycemia / Q000473:pathology*; D007249:Inflammation / Q000473:pathology; D020738:Leptin / Q000378:metabolism; D008264:Macrophages / Q000473:pathology; D042361:Mammary Glands, Human / Q000502:physiology*; D008875:Middle Aged; D017698:Postmenopause; D017697:Premenopause
D004967:Estrogens; D020738:Leptin; D003577:Cytochrome P-450 Enzyme System; D001141:Aromatase