title: Quantitative trait loci associated with maximal exercise endurance in mice.
abstract: The role of genetics in the determination of maximal exercise endurance is unclear. Six- to nine-week-old F2 mice (n = 99; 60 female, 39 male), derived from an intercross of two inbred strains that had previously been phenotyped as having high maximal exercise endurance (Balb/cJ) and low maximal exercise endurance (DBA/2J), were treadmill tested to estimate exercise endurance. Selective genotyping of the F2 cohort (n = 12 high exercise endurance; n = 12 low exercise endurance) identified a significant quantitative trait locus (QTL) on chromosome X (53.7 cM, DXMit121) in the entire cohort and a suggestive QTL on chromosome 8 (36.1 cM, D8Mit359) in the female mice. Fine mapping with the entire F2 cohort and additional informative markers confirmed and narrowed the QTLs. The chromosome 8 QTL (EE8(F)) is homologous with two suggestive human QTLs and one significant rat QTL previously linked with exercise endurance. No effect of sex (P = 0.33) or body weight (P = 0.79) on exercise endurance was found in the F2 cohort. These data indicate that genetic factors in distinct chromosomal regions may affect maximal exercise endurance in the inbred mouse. Whereas multiple genes are located in the identified QTL that could functionally affect exercise endurance, this study serves as a foundation for further investigations delineating the identity of genetic factors influencing maximum exercise endurance.
authors.affiliation: Department of Kinesiology, University of North Carolina-Charlotte, Charlotte, North Carolina 28223, USA. jtlightf@uncc.edu
authors.forename: J Timothy
authors.initials: JT
authors.lastname: Lightfoot
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Turner
authors.forename: Amy Kleinfehn
authors.initials: AK
authors.lastname: Knab
authors.forename: Anne E
authors.initials: AE
authors.lastname: Jedlicka
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Oshimura
authors.forename: Jacqui
authors.initials: J
authors.lastname: Marzec
authors.forename: Wesley
authors.initials: W
authors.lastname: Gladwell
authors.forename: Larry J
authors.initials: LJ
authors.lastname: Leamy
authors.forename: Steven R
authors.initials: SR
authors.lastname: Kleeberger
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D005260:Female; D008040:Genetic Linkage; D005838:Genotype; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D010641:Phenotype; D010807:Physical Endurance / Q000235:genetics*; D040641:Quantitative Trait Loci; D013045:Species Specificity; D014960:X Chromosome