A sensitive sandwich ELISA for buffalo prolactin.
Two murine monoclonal antibodies have been developed against the buffalo prolactin (buPRL). These were designated as 1501 and 1504. Using two MAbs and anti-buPRL rabbit serum, an analysis was performed for the development of a sandwich ELISA (sELISA). The 1504/buPRL/anti-buPRL rabbit serum system was found feasible for sELISA. The sELISA had a sensitivity of 156 pg/mL. The buffalo serum sample showed a parallelism with the standard curve. The intra- and inter-assay coefficients of variance were 8.4% and 9.06%, respectively. These data proved the validity of the assay.
Panchal
Manoj
M
Hormone Research Laboratory, Department of Zoology, University of Delhi, Delhi, India.
Muralidhar
K
K
Gupta
S K
SK
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002020:Buffaloes / Q000097:blood* / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D051379:Mice; D011388:Prolactin / Q000097:blood* / Q000276:immunology; D012098:Reproduction; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D011388:Prolactin