[Association of C1019T polymorphism in the connexin 37 gene and coronary artery disease in Chinese Han population].
OBJECTIVE
To investigate the association between the connexin 37 (CX37) C1019T polymorphism and the susceptibility to coronary artery disease (CAD) in northern Han population of China.


METHODS
A total of 514 CAD patients and 400 healthy controls diagnosed by angiography were genotyped by using polymerase chain reaction-restriction fragment length polymorphism and polyacrylamide gel electrophoresis.


RESULTS
The genotype frequencies of CC, TC and TT in the CX37 C1019T polymorphism was 22.37%, 53.31% and 24.32% in CAD patients, 17.75%, 46.50% and 35.75% in the controls respectively (P = 0.0007). The frequency of the CX37 C allele in CAD patients was significantly higher than that of the control group (49.03% vs 41.00%, OR = 1.38, 95% CI = 1.15 - 1.66, P = 0.0006). The frequency of the C allele carriers (CC + TC) was 75.68% in the CAD group and 64.25% in the control group (P = 0.0002). Compared with the TT homozygote, the CAD risk was significantly increased in the carriers of C allele (CC + TC) (OR = 1.73, 95% CI = 1.30 - 2.30). Subsequent stratified analysis revealed that the frequency of C allele was significantly higher in the male CAD patients than in the male controls (49.37% vs 39.60%, OR = 1.49, 95% CI = 1.18 - 1.89, P = 0.0009). The CAD risk was nearly two-fold increased in the carriers of C allele (CC + TC) than in the TT homozygote (95% CI = 1.38 - 2.78). However in the female population, there was no difference in the CAD risk between the carriers of (CC + TC) type and the TT homozygote (P = 0.24).


CONCLUSIONS
The C allele in the CX37 gene might be associated with the susceptibility to CAD and potentially plays an important role in the manifestation of coronary atherosclerosis among Chinese.
Han
Ya-Ling
YL
Department of Cardiology, Shenyang General Hospital, Cardiovascular Research Institute of PLA, Shenyang 110016, China. hanyal@mail.sy.ln.cn
Xi
Su-Ya
SY
Zhang
Xiao-Lin
XL
Yan
Cheng-Hui
CH
Yang
Yong
Y
Kang
Jian
J
D000328:Adult; D000368:Aged; D000483:Alleles; D001483:Base Sequence; D002681:China; D017630:Connexins / Q000235:genetics*; D003324:Coronary Artery Disease / Q000235:genetics* / Q000473:pathology; D004252:DNA Mutational Analysis; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D000097006:Gap Junction alpha-4 Protein
D017630:Connexins; D000097006:Gap Junction alpha-4 Protein