A method for parental RNA interference in the wasp Nasonia vitripennis.
The wasp Nasonia vitripennis is emerging as a useful model organism in which to address a variety of biological questions, due, in part, to its ease of laboratory use, unique aspects of its biology and the sequencing of its genome. In order to take full advantage of the potential of this organism, methods for manipulating gene function are needed. To this end, a protocol for parental RNA interference (pRNAi) in N. vitripennis is described. pRNAi entails injecting pupae with double-stranded RNA, allowing the injected wasps to eclose and examining the progeny for developmental defects. This basic protocol is described in the context of the life cycle of N. vitripennis. This technique has been useful in elucidating the function of most, although not all, genes tested to date, and has potential applications beyond embryonic patterning. pRNAi experiments in Nasonia can be completed in as little as 2 weeks.
Lynch
Jeremy A
JA
Department of Biology, New York University, 100 Washington Square East, New York, New York 10003, USA.
Desplan
Claude
C
D000818:Animals; D005821:Genetic Techniques; D011679:Pupa / Q000235:genetics; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000502:physiology; D014863:Wasps / Q000235:genetics* / Q000254:growth & development
D012330:RNA, Double-Stranded