Improvement of artemisinin production by chitosan in hairy root cultures of Artemisia annua L.
Artemisinin production by hairy roots of Artemisia annua L. was increased 6-fold to 1.8 microg mg(-1) dry wt over 6 days by adding 150 mg chitosan l(-1). The increase was dose-dependent. Similar treatment of hairy roots with methyl jasmonate (0.2 mM) or yeast extract (2 mg ml(-1)) increased artemisinin production to 1.5 and 0.9 microg mg(-1) dry wt, respectively.
Putalun
Waraporn
W
Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Thailand, waraporn@kku.ac.th
Luealon
Wanwimon
W
De-Eknamkul
Wanchai
W
Tanaka
Hiroyuki
H
Shoyama
Yukihiro
Y
D000085:Acetates / Q000494:pharmacology; D031172:Artemisia annua / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D037621:Artemisinins / Q000378:metabolism*; D048271:Chitosan / Q000494:pharmacology*; D003517:Cyclopentanes / Q000494:pharmacology; D054883:Oxylipins; D018517:Plant Roots / Q000187:drug effects* / Q000378:metabolism*; D013997:Time Factors
D000085:Acetates; D037621:Artemisinins; D003517:Cyclopentanes; D054883:Oxylipins; C072239:methyl jasmonate; D048271:Chitosan; C031327:artemisinin