title: Murine hematopoietic stem cells and progenitors express adrenergic receptors.
abstract: Association between the nervous and immune system is well documented. Immune cells originate within the bone marrow that is innervated. Thermal injury induces adrenergic stimulation, augments monocytopoiesis and alters the beta-adrenergic receptor (AR) profile of bone marrow monocyte committed progenitors. This provides an impetus to study AR expression in hematopoietic progenitors along myeloid lineage. Using FACS analysis and confocal microscopy, we report the expression of alpha1-, alpha2- and beta(2)-AR in enriched populations of ER-MP209(+) and ER-MP12(+) myeloid progenitors, CD117(+) and CD34(+) multi-potential progenitors and more importantly pluripotent stem cells suggesting a plausible role for catecholamine in hematopoietic development.
authors.affiliation: Loyola University Medical Center, Department of Surgery, Building 110, Room 4251, 2160S First Avenue, Maywood, IL 60153, USA. kmuthu@lumc.edu
authors.forename: Kuzhali
authors.initials: K
authors.lastname: Muthu
authors.forename: Sivaraman
authors.initials: S
authors.lastname: Iyer
authors.forename: L-K
authors.initials: LK
authors.lastname: He
authors.forename: Andrea
authors.initials: A
authors.lastname: Szilagyi
authors.forename: Richard L
authors.initials: RL
authors.lastname: Gamelli
authors.forename: Ravi
authors.initials: R
authors.lastname: Shankar
authors.forename: Stephen B
authors.initials: SB
authors.lastname: Jones
mesh_terms: D018663:Adrenergic Agents / Q000494:pharmacology; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001854:Bone Marrow Cells / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D003114:Colony-Forming Units Assay / Q000379:methods; D005434:Flow Cytometry / Q000379:methods; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D008297:Male; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods; D011941:Receptors, Adrenergic / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D018663:Adrenergic Agents; D015703:Antigens, CD; D011941:Receptors, Adrenergic; D014409:Tumor Necrosis Factor-alpha