Heavy metals modulate glutamatergic system in human platelets.
Research strategies have been developed to characterize parameters in peripheral tissues that might easily be measured in humans as surrogate markers of damage, dysfunction or interactions involving neural targets of toxicants. The similarities between platelet and neuron may even be clinically important, as a number of biochemical markers show parallel changes in the central nervous system (CNS) and platelets. The purpose of our research was to investigate the effect of Hg(2+), Pb(2+) and Cd(2+) on the [(3)H]-glutamate binding and [(3)H]-glutamate uptake in human platelets. The involvement of oxidative stress in the modulation of glutamatergic system induced by heavy metals was also investigated. The present study clearly demonstrates that Hg(2+), Cd(2+), and Pb(2+) inhibited [(3)H]-glutamate uptake in human platelets. Hg(2+) inhibited [(3)H]-glutamate binding, while Cd(2+) and Pb(2+) stimulated [(3)H]-glutamate binding in human platelets. Hg(2+), Cd(2+) and Pb(2+) increased lipid peroxidation levels and reactive oxygen species (ROS) measurement in platelets. The present limited results could suggest that glutamatergic system may be used as a potential biomarker for neurotoxic action of heavy metals in humans.
Borges
V C
VC
Departamento de Química, Centro de Ciencias Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Santos
F W
FW
Rocha
J B T
JB
Nogueira
C W
CW
D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism*; D002104:Cadmium / Q000633:toxicity; D018698:Glutamic Acid / Q000097:blood*; D006801:Humans; D066298:In Vitro Techniques; D007854:Lead / Q000633:toxicity; D015227:Lipid Peroxidation / Q000187:drug effects; D008628:Mercury / Q000633:toxicity; D019216:Metals, Heavy / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D019216:Metals, Heavy; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D002104:Cadmium; D007854:Lead; D018698:Glutamic Acid; D008628:Mercury