title: Daam1 regulates the endocytosis of EphB during the convergent extension of the zebrafish notochord.
abstract: Convergent extension (CE) movement of cells is one of the fundamental processes that control the organized morphogenesis of tissues and organs. The molecular events connecting the noncanonical Wnt pathway and CE movement, however, are not well understood. We show that subcellular localization of Daam1, an essential component of noncanonical Wnt signaling, changes dynamically during notochord formation. In the early phases, Daam1 complexes with EphB receptors and Disheveled 2. This complex is incorporated into endocytic vesicles in a dynamin-dependent manner, thereby resulting in the removal of EphB from the cell surface with subsequent switching of cell adhesiveness. In the next step, Daam1 colocalizes with the actin cytoskeleton to induce morphological extension of cells. We elucidate the molecular mechanism underlying the CE movement of notochord cells with Daam1 as a dynamic coordinator of endocytosis and cytoskeletal remodeling.
authors.affiliation: Department of Developmental Neurobiology, Institute of Development, Aging, and Cancer, Tohoku University, 4-1, Seiryo, Aoba, Sendai, Miyagi 980-8575, Japan.
authors.forename: Yasuyuki S
authors.initials: YS
authors.lastname: Kida
authors.forename: Takayuki
authors.initials: T
authors.lastname: Sato
authors.forename: Kota Y
authors.initials: KY
authors.lastname: Miyasaka
authors.forename: Asami
authors.initials: A
authors.lastname: Suto
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Ogura
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D003599:Cytoskeleton / Q000737:chemistry / Q000378:metabolism; D000072261:Dishevelled Proteins; D004705:Endocytosis / Q000502:physiology*; D006801:Humans; D009672:Notochord / Q000737:chemistry / Q000196:embryology* / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism / Q000502:physiology; D036081:Receptors, Eph Family / Q000378:metabolism* / Q000502:physiology; D013347:Subcellular Fractions / Q000737:chemistry / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000378:metabolism / Q000502:physiology*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000072261:Dishevelled Proteins; D010750:Phosphoproteins; D029961:Zebrafish Proteins; D036081:Receptors, Eph Family