title: Androgenic/estrogenic balance in the male rat cerebral circulation: metabolic enzymes and sex steroid receptors.
abstract: Tissues from males can be regulated by a balance of androgenic and estrogenic effects because of local metabolism of testosterone and expression of relevant steroid hormone receptors. As a critical first step to understanding sex hormone influences in the cerebral circulation of males, we investigated the presence of enzymes that metabolize testosterone to active products and their respective receptors. We found that cerebral blood vessels from male rats express 5alpha-reductase type 2 and aromatase, enzymes responsible for conversion of testosterone into dihydrotestosterone (DHT) and 17beta-estradiol, respectively. Protein levels of these enzymes, however, were not modulated by long-term in vivo hormone treatment. We also showed the presence of receptors for both androgens (AR) and estrogens (ER) from male cerebral vessels. Western blot analysis showed bands corresponding to the full-length AR (110 kDa) and ERalpha (66 kDa). Long-term in vivo treatment of orchiectomized rats with testosterone or DHT, but not estrogen, increased AR levels in cerebral vessels. In contrast, ERalpha protein levels were increased after in vivo treatment with estrogen but not testosterone. Fluorescent immunostaining revealed ERalpha, AR, and 5alpha-reductase type 2 in both the endothelial and smooth muscle layers of cerebral arteries, whereas aromatase staining was solely localized to the endothelium. Thus, cerebral vessels from males are target tissues for both androgens and estrogen. Furthermore, local metabolism of testosterone might balance opposing androgenic and estrogenic influences on cerebrovascular as well as brain function in males.
authors.affiliation: Department of Pharmacology, School of Medicine, University of California, Irvine, CA, USA.
authors.forename: Rayna J
authors.initials: RJ
authors.lastname: Gonzales
authors.forename: Saema
authors.initials: S
authors.lastname: Ansar
authors.forename: Sue P
authors.initials: SP
authors.lastname: Duckles
authors.forename: Diana N
authors.initials: DN
authors.lastname: Krause
mesh_terms: D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000378:metabolism; D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D001141:Aromatase / Q000378:metabolism; D015153:Blotting, Western; D001835:Body Weight / Q000502:physiology; D001921:Brain / Q000201:enzymology*; D002560:Cerebrovascular Circulation / Q000502:physiology*; D003627:Data Interpretation, Statistical; D013196:Dihydrotestosterone / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D047628:Estrogen Receptor alpha / Q000378:metabolism; D004967:Estrogens / Q000494:pharmacology*; D012739:Gonadal Steroid Hormones / Q000378:metabolism*; D008297:Male; D018613:Microscopy, Confocal; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D009919:Orchiectomy; D051381:Rats; D011916:Rats, Inbred F344; D011944:Receptors, Androgen / Q000378:metabolism; D011987:Receptors, Steroid / Q000378:metabolism*; D013739:Testosterone / Q000494:pharmacology
chemical_list: D000728:Androgens; D047628:Estrogen Receptor alpha; D004967:Estrogens; D012739:Gonadal Steroid Hormones; D011944:Receptors, Androgen; D011987:Receptors, Steroid; D013196:Dihydrotestosterone; D013739:Testosterone; D004958:Estradiol; D001141:Aromatase; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase