Smallpox subunit vaccine produced in Planta confers protection in mice.
We report here the in planta production of the recombinant vaccinia virus B5 antigenic domain (pB5), an attractive component of a subunit vaccine against smallpox. The antigenic domain was expressed by using efficient transient and constitutive plant expression systems and tested by various immunization routes in two animal models. Whereas oral administration in mice or the minipig with collard-derived insoluble pB5 did not generate an anti-B5 immune response, intranasal administration of soluble pB5 led to a rise of B5-specific immunoglobulins, and parenteral immunization led to a strong anti-B5 immune response in both mice and the minipig. Mice immunized i.m. with pB5 generated an antibody response that reduced virus spread in vitro and conferred protection from challenge with a lethal dose of vaccinia virus. These results indicate the feasibility of producing safe and inexpensive subunit vaccines by using plant production systems.
Golovkin
Maxim
M
Biotechnology Foundation Laboratories, Thomas Jefferson University, Philadelphia, PA 19107-6799, USA.
Spitsin
Sergei
S
Andrianov
Vyacheslav
V
Smirnov
Yuriy
Y
Xiao
Yuhong
Y
Pogrebnyak
Natalia
N
Markley
Karen
K
Brodzik
Robert
R
Gleba
Yuri
Y
Isaacs
Stuart N
SN
Koprowski
Hilary
H
D000281:Administration, Intranasal; D000284:Administration, Oral; D000818:Animals; D001937:Brassica / Q000235:genetics / Q000276:immunology; D002772:Cholera Toxin / Q000008:administration & dosage / Q000276:immunology; D005260:Female; D007273:Injections, Intramuscular; D008562:Membrane Glycoproteins / Q000008:administration & dosage / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D030821:Plants, Genetically Modified / Q000276:immunology*; D012899:Smallpox / Q000276:immunology / Q000517:prevention & control*; D012900:Smallpox Vaccine / Q000008:administration & dosage / Q000096:biosynthesis / Q000276:immunology*; D013552:Swine; D013556:Swine, Miniature; D014026:Nicotiana / Q000235:genetics / Q000276:immunology; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000096:biosynthesis / Q000235:genetics; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000096:biosynthesis / Q000276:immunology; D014616:Vaccinia virus / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000008:administration & dosage / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology*
D008562:Membrane Glycoproteins; D012900:Smallpox Vaccine; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; C074512:42kDa protein, Vaccinia virus; D002772:Cholera Toxin