Influence of endogenous and synthetic female sex hormones on human blood cells in vitro studied with comet assay.
The comet assay has been conducted with numerous cell lines to assess in vitro genotoxicity. In order to use the comet assay as part of an in vitro test for evaluating genotoxicity, however, there are cell-specific factors that need to be better understood. In this present study we have evaluated some factors that may impact upon the DNA damage detected in whole blood (WB) cells and lymphocytes (ILs). Experiments were conducted comparing responses of both cells, and investigating the effects of the female hormonal cycle, and oral contraceptive (OC) use on DNA damage detection in the in vitro comet assay, at three sampling time. No significant differences were detected in the basal levels of DNA damage detected in ILs and WB cells from women OC users and non-users and from men. Basal DNA damage in ILs was unaffected by gender and stage of the menstrual cycle or the stage of the treatment schedule. Our results also indicated that the H2O2 induces DNA damage in human lymphocytes independently of gender, low-dose OC use and hormonal fluctuation. However, data showed that in 3rd sampling of menstrual cycle, lymphocytes were more resistant to H2O2-induced DNA damage than those from OC users and men.
Braz
Mariana Gobbo
MG
Department of Pathology, Faculty of Medicine, UNESP - São Paulo State University, Rubião Junior, 18618-000 Botucatu, SP, Brazil. mgbraz@hotmail.com
Fávero Salvadori
Daisy Maria
DM
D000328:Adult; D001773:Blood Cells / Q000187:drug effects*; D002469:Cell Separation; D020552:Comet Assay; D003278:Contraceptives, Oral, Hormonal / Q000633:toxicity*; D004249:DNA Damage; D004967:Estrogens / Q000502:physiology* / Q000633:toxicity*; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D008598:Menstruation / Q000502:physiology*; D009153:Mutagens; D016877:Oxidants / Q000633:toxicity; D012727:Sex Characteristics
D003278:Contraceptives, Oral, Hormonal; D004967:Estrogens; D009153:Mutagens; D016877:Oxidants; D006861:Hydrogen Peroxide