Microarray cDNA to identify inflammatory genes in nasal polyposis.
BACKGROUND
The objective of this study was to investigate the spectrum of inflammatory gene expression in patients with nasal polyposis.


METHODS
The cDNA microarray technique was used to identify gene expression in tissue samples from nasal polyps and adjacent inflammatory nasal mucosa of 21 patients with nonallergic nasal polyposis. To validate the microarray analysis, we compared the expression of five genes by reverse transcription-polymerase chain reaction (RT-PCR): tumor necrosis factor, IL-5, IL-9, fibroblast growth factor, and transforming growth factor (TGF)-beta1.


RESULTS
We tested 96 different inflammatory genes in our samples. Thirty-six genes exhibited differences in expression between the two tissue types. In all 36 genes the level of expression was greater in the inflammatory mucosa than the polyps. The RT-PCR confirmed the cDNA results.


CONCLUSIONS
We believe that the high expression of TGF-beta1 in inflammatory mucosa compared with the low expression in polyps may reflect an important role in the inhibitory mechanisms of nasal polyposis.
Figueiredo
Claudia R
CR
Department of Otorhinolaryngology, Head and Neck Surgery, Federal University of São Paulo, Brazil. claufig@uol.com.br
Santos
Rodrigo P
RP
Silva
Ismael D C G
ID
Weckx
Luc L M
LL
D000328:Adult; D000368:Aged; D016207:Cytokines / Q000235:genetics*; D005260:Female; D005346:Fibroblast Growth Factors / Q000235:genetics; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D006801:Humans; D007249:Inflammation / Q000235:genetics*; D015848:Interleukin-5 / Q000235:genetics; D016906:Interleukin-9 / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000473:pathology*; D009298:Nasal Polyps / Q000235:genetics* / Q000473:pathology*; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D053773:Transforming Growth Factor beta1 / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D016207:Cytokines; D015848:Interleukin-5; D016906:Interleukin-9; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha; D005346:Fibroblast Growth Factors; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases