Plant protein extraction.
A method is presented for the extraction of total protein from Arabidopsis thaliana tissue. The protocol was designed for the solubilization of a range of proteins and their efficient and quantitative recovery. It is especially compatible with the small quantities of available tissue often associated with this species and was originally intended for Western blot preparations. Samples extracted using this method can be quantitated directly using a commercially available kit.
Conlon
Helen E
HE
Department of Biology, University of Leicester, UK.
Salter
Michael G
MG
D017360:Arabidopsis / Q000737:chemistry; D029681:Arabidopsis Proteins / Q000302:isolation & purification*; D015153:Blotting, Western; D012967:Sodium Dodecyl Sulfate; D012995:Solubility
D029681:Arabidopsis Proteins; D012967:Sodium Dodecyl Sulfate