title: Choice of cranial window type for in vivo imaging affects dendritic spine turnover in the cortex.
abstract: Determining the degree of synapse formation and elimination is essential for understanding the structural basis of brain plasticity and pathology. We show that in vivo imaging of dendritic spine dynamics through an open-skull glass window, but not a thinned-skull window, is associated with high spine turnover and substantial glial activation during the first month after surgery. These findings help to explain existing discrepancies in the degree of dendritic spine plasticity observed in the mature cortex.
authors.affiliation: Molecular Neurobiology Program, Skirball Institute, Department of Physiology and Neuroscience, New York University School of Medicine, 540 First Avenue, New York, New York 10016, USA.
authors.forename: Hua-Tai
authors.initials: HT
authors.lastname: Xu
authors.forename: Feng
authors.initials: F
authors.lastname: Pan
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.forename: Wen-Biao
authors.initials: WB
authors.lastname: Gan
mesh_terms: D000818:Animals; D000073883:CX3C Chemokine Receptor 1; D003399:Craniotomy / Q000379:methods; D049229:Dendritic Spines / Q000378:metabolism / Q000648:ultrastructure*; D003952:Diagnostic Imaging / Q000379:methods; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D018613:Microscopy, Confocal / Q000379:methods; D009457:Neuroglia / Q000378:metabolism; D009473:Neuronal Plasticity / Q000502:physiology*; D019707:Receptors, Chemokine / Q000235:genetics / Q000378:metabolism; D013003:Somatosensory Cortex / Q000166:cytology*; D013997:Time Factors
chemical_list: D000073883:CX3C Chemokine Receptor 1; C471885:Cx3cr1 protein, mouse; D005904:Glial Fibrillary Acidic Protein; D008164:Luminescent Proteins; D019707:Receptors, Chemokine; D049452:Green Fluorescent Proteins