Investigation of SNPs in the porcine desmoglein 1 gene.
BACKGROUND
Desmoglein 1 (DSG1) is the target protein in the skin disease exudative epidermitis in pigs caused by virulent strains of Staphylococcus hyicus. The exfoliative toxins produced by S. hyicus digest the porcine desmoglein 1 (PIG)DSG1 by a very specific reaction. This study investigated the location of single nucleotide polymorphisms (SNPs) in the porcine desmoglein 1 gene (PIG)DSG1 in correlation to the cleavage site as well as if the genotype of the SNPs is correlated to susceptibility or resistance to the disease.


RESULTS
DNA from 32 affected and 32 unaffected piglets with exudative epidermitis were diagnosed clinically as affected or unaffected. Two regions of the desmoglein 1 gene were sequenced and genotypes of the SNPs were established. Seven SNPs (823T>C, 828A>G, 829A>G, 830A>T, 831A>T, 838A>C and 1139C>T) were found in the analysed sequences and the allele frequencies were determined for the SNPs resulting in amino acid change. Four of the seven polymorphisms were situated in the motif known to be important for toxin cleavage. The distribution of the genotypes between affected and unaffected animals was analysed.


CONCLUSIONS
The study indicated a possible correlation between the genotypes of two out of seven SNPs found in the porcine desmoglein 1 gene and the susceptibility to exudative epidermitis.
Daugaard
Lise
L
The National Veterinary Institute, Technical University of Denmark, Bülowsvej 27, Copenhagen V, Denmark. lid@vet.dtu.dk
Andresen
Lars Ole
LO
Fredholm
Merete
M
D020816:Amino Acid Motifs / Q000502:physiology; D000818:Animals; D001427:Bacterial Toxins / Q000378:metabolism; D001483:Base Sequence; D016022:Case-Control Studies; D017931:DNA Primers / Q000737:chemistry; D051183:Desmoglein 1 / Q000737:chemistry / Q000235:genetics*; D004818:Epidermitis, Exudative, of Swine / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D013552:Swine
D001427:Bacterial Toxins; D017931:DNA Primers; D051183:Desmoglein 1