Luminescence of a Pt(II) complex in the presence of DNA. Dependence of luminescence changes on the interaction binding mode.
Luminescence intensity changes of a Pt(II) complex which is known to bind externally to DNA at low [DNA]/[complex] ratio and to intercalate at high [DNA]/[complex] ratio are studied in the presence of calf thymus DNA. External binding is demonstrated to induce luminescence enhancement whereas intercalation leads to luminescence quenching. The reasons for this behaviour are discussed.
Puntoriero
Fausto
F
Dipartimento di Chimica Inorganica, Chimica Analitica e Chimica Fisica, Università di Messina, Salita Sperone 31, 98166, Vill. S. Agata, Messina.
Campagna
Sebastiano
S
Di Pietro
Maria Letizia
ML
Giannetto
Antonino
A
Cusumano
Matteo
M
D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D007364:Intercalating Agents / Q000138:chemical synthesis / Q000737:chemistry*; D049449:Luminescence; D049408:Luminescent Agents / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D009944:Organoplatinum Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D011119:Polynucleotides / Q000737:chemistry; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D007364:Intercalating Agents; D049408:Luminescent Agents; D009944:Organoplatinum Compounds; D011119:Polynucleotides; D004247:DNA; C427203:calf thymus DNA