Most human Alu and murine B1 repeats are unique.
Alus and B1s are short interspersed repeat elements (SINEs) indirectly derived from the 7SL RNA gene. While most researchers recognize that there exists extensive variability between individual elements, the extent of this variability has never been systematically tested. We examined all Alu elements over 200 nucleotides and all B1 elements over 100 nucleotides in the human and mouse genomes, and analyzed the number of copies of each element at various stringencies from 22 nucleotides to full length. Over 98% of 923,277 Alus and 365,377 B1s examined were unique when queried at full length. When the criterion was reduced to half the length of the repeat, 97% of the Alus and 73% of the B1s were still found to be a single copy. All single and multi-copy sequences have been mapped and documented. Access to the data is possible using the AluPlus website http://www.ibr.hawaii.edu.
Umylny
Boris
B
Institute of Biogenesis Research, John A. Burns School of Medicine, University of Hawaii, Honolulu, Hawaii 96822, USA.
Presting
Gernot
G
Efird
Jimmy T
JT
Klimovitsky
Boris I
BI
Ward
W Steven
WS
D020087:Alu Elements; D000818:Animals; D033481:Chromosomes, Mammalian / Q000737:chemistry; D030561:Databases, Nucleic Acid; D020862:GC Rich Sequence; D014644:Genetic Variation; D023281:Genomics; D006801:Humans; D020407:Internet; D051379:Mice; D017384:Sequence Deletion; D020086:Short Interspersed Nucleotide Elements