[Effect of calcium on reconstruction of basement membrane in composite chitosan tissue engineering skin].
OBJECTIVE
To search the most suitable concentration of calcium in the medium for the basement membrane reconstruction in tissue engineering skin in vitro.


METHODS
Composite chitosan tissue engineering skin was prepared according to previous studies. Four groups were included according to the concentration of calcium (1.00, 1.45, 1.65 and 1.95 mmol/L respectively). After 7 days and 15 days of culture, the histological manifestation of basement membrane in tissue engineering skin was observed by hematoxylin & eosin staining and PAS staining, and collagen IV of basement membrane was detected immunohistochemically at the dermal-epidermal junction.


RESULTS
This tissue engineering skin shared some histological features of normal skin, including a well-differentiated stratified epidermis and a dense dermis. The epithelium in the group of 1.95 mmol/L. Llcium differentiated better than those in other groups. PAS staining showing a regularly red dying strap domain at the dermal-epidermal junction. Collagen IV was positively stained immunohistochemically at the dermal-epidermal junction in the tissue engineering skin.


CONCLUSIONS
The above results suggest that the medium with 1.95 mmol/L calcium should be suitable for the growth of composite chitosan tissue engineering skin and the reconstruction of basement membrane.
Lu
Yuangang
Y
Department of Dermatology, Daping Hospital, Third Military Medical University, Chongqing, 400042, PR China.
Wu
Jinjin
J
Lei
Xia
X
D001485:Basement Membrane / Q000166:cytology* / Q000648:ultrastructure; D002118:Calcium / Q000737:chemistry*; D049109:Cell Proliferation; D002478:Cells, Cultured; D048271:Chitosan; D003470:Culture Media; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000166:cytology*; D017768:Skin, Artificial; D023822:Tissue Engineering
D003470:Culture Media; D048271:Chitosan; D002118:Calcium