Comparison of tests for detection of beta-lactamase-producing staphylococci.
Penicillin resistance identification tests are important in veterinary medicine. Six enzyme assays and a PCR test were compared for the detection of beta-lactamase production or the beta-lactamase gene in 175 staphylococcal isolates. We conclude that the PCR test and two nitrocefin-based assays can be recommended for routine clinical use.
Pitkälä
A
A
Finnish Food Safety Authority Evira, Mustialankatu 3, 00790 Helsinki, Finland. anna.pitkala@evira.fi
Salmikivi
L
L
Bredbacka
P
P
Myllyniemi
A-L
AL
Koskinen
M T
MT
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D002511:Cephalosporins / Q000494:pharmacology*; D003030:Coagulase / Q000378:metabolism; D003124:Colorimetry / Q000379:methods; D007202:Indicators and Reagents / Q000494:pharmacology*; D008414:Mastitis, Bovine / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary; D013210:Staphylococcus / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
D002511:Cephalosporins; D003030:Coagulase; D007202:Indicators and Reagents; D001618:beta-Lactamases; C021720:nitrocefin