title: Chlamydomonas reinhardtii CNX1E reconstitutes molybdenum cofactor biosynthesis in Escherichia coli mutants.
abstract: We have isolated and characterized the Chlamydomonas reinhardtii genes for molybdenum cofactor biosynthesis, namely, CNX1G and CNX1E, and expressed them and their chimeric fusions in Chlamydomonas and Escherichia coli. In all cases, the wild-type phenotype was restored in individual mutants as well as in a CNX1G CNX1E double mutant. Therefore, CrCNX1E is the first eukaryotic protein able to complement an E. coli moeA mutant.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Córdoba, Campus de Rabanales, Edificio Severo Ochoa, 14071 Córdoba, Spain.
authors.forename: Angel
authors.initials: A
authors.lastname: Llamas
authors.forename: Manuel
authors.initials: M
authors.lastname: Tejada-Jimenez
authors.forename: David
authors.initials: D
authors.lastname: González-Ballester
authors.forename: José Javier
authors.initials: JJ
authors.lastname: Higuera
authors.forename: Guenter
authors.initials: G
authors.lastname: Schwarz
authors.forename: Aurora
authors.initials: A
authors.lastname: Galván
authors.forename: Emilio
authors.initials: E
authors.lastname: Fernández
mesh_terms: D020418:Algal Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000235:genetics / Q000378:metabolism*; D003067:Coenzymes / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005816:Genetic Complementation Test; D008667:Metalloproteins / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D000090006:Molybdenum Cofactors; D009687:Nuclear Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D015800:Protozoan Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D011621:Pteridines; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D020418:Algal Proteins; D003067:Coenzymes; D008667:Metalloproteins; D000090006:Molybdenum Cofactors; D009687:Nuclear Proteins; D015800:Protozoan Proteins; D011621:Pteridines; D011993:Recombinant Fusion Proteins; C022261:molybdopterin