title: Capture ELISA quantitation of mouse adenovirus type 1 in infected organs.
abstract: A capture enzyme-linked immunosorbent assay (ELISA) was optimized for identification of mouse adenovirus type 1 in infected brain homogenates. The ELISA method allows for a much faster quantitation of virus in infected organs than plaque assays. Methods for organ homogenization and the subsequent capture ELISA are described in this chapter.
authors.affiliation: Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA.
authors.forename: Amanda R
authors.initials: AR
authors.lastname: Welton
authors.forename: Katherine R
authors.initials: KR
authors.lastname: Spindler
mesh_terms: D000256:Adenoviridae / Q000302:isolation & purification*; D000257:Adenoviridae Infections / Q000175:diagnosis*; D000818:Animals; D001921:Brain / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007202:Indicators and Reagents; D051379:Mice; D012449:Safety; D012680:Sensitivity and Specificity; D010948:Viral Plaque Assay
chemical_list: D007202:Indicators and Reagents