[Relations of platelet phospholipid fatty acids to thrombotic risk factors in middle-aged and geriatric patients with hyperlipidemia].
OBJECTIVE
To investigate the relations of platelet phospholipid fatty acids to thrombotic risk factors in the middle-aged and geriatric patients with hyperlipidemia in the metropolitan area of Hangzhou, Zhejiang province.


METHODS
A questionnaire survey was conducted among 81 patients with hyperlipidemia, 50 males and 31 females, aged (57 +/- 8), and 65 healthy controls, 43 males and 22 females, aged (58 +/- 9) to collect the data about height, weight, and diet. Peripheral venous blood samples were collected to examine the total cholesterol, triglyceride, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), homocysteine (Hcy), 6-keto-prostaglandin F(1alpha) (6-keto-PGF(1alpha)), and thromboxane B(2) (TXB(2)) were examined by standard methods. Serum thrombotic risk factors including homocysteine and Thromboxane B(2) were determined by standard methods. Platelet phospholipid fatty acids were examined by gas chromatography. The correlation between the serum thrombotic risk factors (Hcy, TXB(2), and 6-keto-PG F1a) was analyzed by multivariate linear regression.


RESULTS
There were no significant differences in platelet phospholipid fatty acids between the patients with hyperlipidemia and the healthy controls. Serum Hcy was significantly negatively correlated with docosahexaenoic acid (DHA) and the ratio of n-3 PUFA (polyunsaturated fatty acids)/n-6 PUFA (r = -0.277 and -0.231, both P < 0.01). The level of serum TXB(2) was significantly positively correlated with arachidonic acid (r = 0.176, P < 0.05), and significantly negatively correlated with DHA (r = -0.209, P < 0.01), eicosapentaenoic acid (EPA) (r = -0.194, P < 0.05), and n-3 PUFA/n-6 PUFA (r = -0.238, P < 0.01).


CONCLUSIONS
Increasing the ratio of n-3 PUFA/n-6 PUFA in platelet phospholipid may potentially decrease the thrombotic risks such as Hcy and TXB(2) and provide a reference for diet selection.
Yu
Xiao-Mei
XM
Department of Clinical Laboratory, Zhejiang Hospital, Hangzhou 310013, China.
Li
Duo
D
Xie
Hai-Bao
HB
Zhang
Yong-Hua
YH
Wang
Qiang
Q
Zhou
Xiao-Qiong
XQ
Yu
Peng
P
Wang
Li-Jun
LJ
D015121:6-Ketoprostaglandin F1 alpha / Q000097:blood; D000368:Aged; D016718:Arachidonic Acid / Q000097:blood; D001792:Blood Platelets / Q000737:chemistry; D002784:Cholesterol / Q000097:blood; D004041:Dietary Fats / Q000097:blood; D005227:Fatty Acids / Q000097:blood*; D005260:Female; D006710:Homocysteine / Q000097:blood; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood*; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D010743:Phospholipids / Q000097:blood*; D012307:Risk Factors; D011795:Surveys and Questionnaires; D013927:Thrombosis / Q000097:blood*; D013929:Thromboxane B2 / Q000097:blood
D004041:Dietary Fats; D005227:Fatty Acids; D010743:Phospholipids; D006710:Homocysteine; D016718:Arachidonic Acid; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D002784:Cholesterol