Prevention of pathogenic Escherichia coli infection in mice and stimulation of macrophage activation in rats by an oral administration of probiotic Lactobacillus casei I-5.
Lactobacillus casei I-5 isolated from an alcohol fermentation broth enhanced immunity and prevented pathogenic infection as a probiotic. Mice fed with I-5 cells for 11 days prior to an intraperitoneal challenge with pathogenic Escherichia coli Juhl exhibited a high survival rate compared with the control group. Rats fed with I-5 cells for 10 days significantly increased the phagocytosis of peritoneal macrophages. In a cell culture system employing peritoneal macrophages from rats, the I-5 administration activated NF-kappaB stimulated by LPS. It also enhanced LPS-stimulated IL-12 and TNF-alpha production, but not IL-6 production. These results show that L. casei I-5 effectively prevented infection by pathogenic E. coli possibly through the activation of peritoneal macrophages. The strain would be useful to prevent pathogenic microbial infections in humans and farm animals.
Ishida-Fujii
Keiko
K
R & D Center, Japan Alcohol Corporation, Chiba, Japan.
Sato
Rieko
R
Goto
Shingo
S
Yang
Xiao-Ping
XP
Kuboki
Hiroshi
H
Hirano
Shin-Ichi
S
Sato
Michikatsu
M
D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D002478:Cells, Cultured; D016207:Cytokines / Q000097:blood; D003602:Cytotoxicity, Immunologic; D004927:Escherichia coli Infections / Q000517:prevention & control*; D005243:Feces / Q000737:chemistry; D007111:Immunity, Cellular / Q000276:immunology; D007070:Immunoglobulin A / Q000032:analysis; D007136:Immunoglobulins / Q000097:blood; D007694:Killer Cells, Natural / Q000276:immunology; D007780:Lacticaseibacillus casei / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation / Q000502:physiology*; D017737:Macrophages, Peritoneal / Q000276:immunology; D051379:Mice; D008813:Mice, Inbred ICR; D016328:NF-kappa B / Q000096:biosynthesis; D010587:Phagocytosis / Q000502:physiology; D019936:Probiotics; D051381:Rats
D016207:Cytokines; D007070:Immunoglobulin A; D007136:Immunoglobulins; D008070:Lipopolysaccharides; D016328:NF-kappa B