Use of protein-specific monoclonal antibody-based latex agglutination for rapid diagnosis of Burkholderia pseudomallei infection in patients with community-acquired septicemia.
A latex agglutination test employing monoclonal antibody specific to a 30-kDa protein of Burkholderia pseudomallei was used to detect the organisms in blood culture specimens from 1,139 patients with community-acquired septicemia. The sensitivity, specificity, and positive and negative predictive values of the test were 96.75%, 99.61%, 96.75%, and 99.61%, respectively.
Ekpo
Pattama
P
Department of Immunology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand. sipep@mahidol.ac.th
Rungpanich
Utane
U
Pongsunk
Supinya
S
Naigowit
Pimjai
P
Petkanchanapong
Vimon
V
D000911:Antibodies, Monoclonal / Q000276:immunology*; D016470:Bacteremia / Q000175:diagnosis* / Q000382:microbiology; D016957:Burkholderia pseudomallei / Q000276:immunology*; D017714:Community-Acquired Infections / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D007841:Latex Fixation Tests / Q000379:methods*; D008554:Melioidosis / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D013997:Time Factors
D000911:Antibodies, Monoclonal