Oat beta-glucan effects on neutrophil respiratory burst activity following exercise.
Fatiguing exercise has been associated with a decrease in certain functions of neutrophils, whereas moderate exercise has generally been associated with an increase. Consumption of oat beta-glucan (ObetaG), a soluble fiber and mild immune system enhancer, may offset the immunosuppression associated with intense training and perhaps further enhance the benefits of moderate exercise.


OBJECTIVE
To test the effects of ObetaG consumption on neutrophil function and number after both moderate and fatiguing exercise.


METHODS
Male mice were assigned to one of six treatment groups. Fatiguing exercise mice (Ftg-ObetaG and Ftg-H2O) ran to volitional fatigue on a treadmill for three consecutive days, and moderate exercise mice (Mod-ObetaG and Mod-H2O) ran for six consecutive days for 1 h. Control mice (Con-ObetaG and Con-H2O) were exposed to the treadmill environment but did not run. ObetaG was consumed in the drinking water (approximately 0.6 mL x d(-1)) for 10 consecutive days. After rest or exercise on the last day of training, mice were given a 1-mL i.p. injection of thioglycollate. Mice were sacrificed 3 h later; neutrophils were harvested from the peritoneal cavity and counted, and their respiratory burst activity was measured using flow cytometry.


RESULTS
Both moderate exercise and ObetaG increased neutrophil burst activity, whereas fatiguing exercise had no effect. Neutrophil number was increased by fatiguing exercise and ObetaG, but not moderate exercise. There were no additive effects of exercise and ObetaG on either of these variables.


CONCLUSIONS
These data suggest that although not additive in their effects, both ObetaG and exercise can alter overall neutrophil respiratory burst activity (number and/or function), but only ObetaG increased both number and function, which may have important ramifications for defense against infection.
Murphy
E Angela
EA
Divison of Applied Physiology, Department of Exercise Science, Arnold School of Public Health, University of South Carolina, Columbia, SC 29208, USA.
Davis
J Mark
JM
Brown
Adrienne S
AS
Carmichael
Martin D
MD
Ghaffar
Abdul
A
Mayer
Eugene P
EP
D000818:Animals; D018554:Avena / Q000737:chemistry* / Q000276:immunology; D007108:Immune Tolerance; D008297:Male; D051379:Mice; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D010805:Physical Conditioning, Animal; D016897:Respiratory Burst / Q000502:physiology*; D013022:South Carolina; D047071:beta-Glucans / Q000494:pharmacology*
D047071:beta-Glucans; C067858:beta-glucan, (1-3)(1-4)-