title: Development of high-throughput phenotyping of metagenomic clones from the human gut microbiome for modulation of eukaryotic cell growth.
abstract: Metagenomic libraries derived from human intestinal microbiota (20,725 clones) were screened for epithelial cell growth modulation. Modulatory clones belonging to the four phyla represented among the metagenomic libraries were identified (hit rate, 0.04 to 8.7% depending on the screening cutoff). Several candidate loci were identified by transposon mutagenesis and subcloning.
authors.affiliation: Unité d'Ecologie et de Physiologie du Système Digestif, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France.
authors.forename: Karine
authors.initials: K
authors.lastname: Gloux
authors.forename: Marion
authors.initials: M
authors.lastname: Leclerc
authors.forename: Harout
authors.initials: H
authors.lastname: Iliozer
authors.forename: René
authors.initials: R
authors.lastname: L'Haridon
authors.forename: Chaysavanh
authors.initials: C
authors.lastname: Manichanh
authors.forename: Gérard
authors.initials: G
authors.lastname: Corthier
authors.forename: Renaud
authors.initials: R
authors.lastname: Nalin
authors.forename: Hervé M
authors.initials: HM
authors.lastname: Blottière
authors.forename: Joël
authors.initials: J
authors.lastname: Doré
mesh_terms: D000818:Animals; D001419:Bacteria / Q000235:genetics*; D002460:Cell Line; D049109:Cell Proliferation; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004847:Epithelial Cells / Q000382:microbiology; D005057:Eukaryotic Cells / Q000382:microbiology*; D015870:Gene Expression; D016680:Genome, Bacterial; D023281:Genomics / Q000379:methods*; D006801:Humans; D007422:Intestines / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial