Excretion profile of boldenone and its metabolites after oral administration to veal calves.
The residue profiles of boldenone (17beta-Bol), its epimer (17alpha-Bol) and the related compound androsta-1,4-diene-3,17-dione (ADD), were investigated by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in urine of male calves orally treated with boldenone, boldenone esters, and/or ADD. In all the experiments with the administered steroids residues of 17alpha-Bol decreased rapidly after end of treatment; detectable amounts of 17alpha-Bol were however noticed along the withdrawal observation period after end of treatment. Differently, residues of 17beta-Bol were detectable only shortly after administration. This in vivo research concerning oral treatments of cattle with boldenone related substances proves ADD to be a very active boldenone precursor in bovine animals.
Ferretti
G
G
Istituto Superiore di Sanità, Department of Food Safety and Veterinary Public Health, Roma, Italy.
Palleschi
L
L
Marchiafava
C
C
delli Quadri
F
F
Fantozzi
L
L
Ferranti
C
C
Cammarata
P
P
Macrì
A
A
Montesissa
C
C
Draisci
R
R
D000284:Administration, Oral; D045930:Anabolic Agents / Q000008:administration & dosage / Q000652:urine*; D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods; D008297:Male; D053719:Tandem Mass Spectrometry / Q000379:methods; D013739:Testosterone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000652:urine
D045930:Anabolic Agents; D013739:Testosterone; C013915:boldenone