title: Detection of heavy metal toxicity using cardiac cell-based biosensor.
abstract: Biosensors incorporating mammalian cells have a distinct advantage of responding in a manner which offers insight into the physiological effect of an analyte. To investigate the potential applications of cell-based biosensors on heavy metal toxicity detection, a novel biosensor for monitoring electrophysiological activity was developed by light-addressable potentiometric sensor (LAPS). Extracellular field potentials of spontaneously beating cardiomyocytes could be recorded by LAPS in the range of 20 microV to nearly 40 microV with frequency of 0.5-3 Hz. After exposed to different heavy metal ions (Hg(2+), Pb(2+), Cd(2+), Fe(3+), Cu(2+), Zn(2+); in concentration of 10 microM), cardiomyocytes demonstrated characteristic changes in terms of beating frequency, amplitude and duration under the different toxic effects of ions in less than 15 min. This study suggests that, with the physiological monitoring, it is possible to use the cardiac cell-based biosensor to study acute and eventually chronic toxicities induced by heavy metal ions in a long-term and no-invasive way.
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, PR China.
authors.forename: Qingjun
authors.initials: Q
authors.lastname: Liu
authors.forename: Hua
authors.initials: H
authors.lastname: Cai
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.forename: Lidan
authors.initials: L
authors.lastname: Xiao
authors.forename: Mo
authors.initials: M
authors.lastname: Yang
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002478:Cells, Cultured; D008027:Light; D019216:Metals, Heavy / Q000633:toxicity*; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000502:physiology; D011199:Potentiometry / Q000295:instrumentation*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D019216:Metals, Heavy