title: Purification, characterization, and molecular cloning of a thermostable superoxide dismutase from Thermoascus aurantiacus.
abstract: A thermostable superoxide dismutase [(SOD) EC 1.15.1.1] from a Thermoascus aurantiacus var. levisporus was purified to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) homogeneity by a series of column chromatographies. The molecular mass of a single band of the enzyme was estimated to be 16.8 kDa by SDS-PAGE. The molecular mass was estimated to be 33.2 kDa by gel filtration on Sephacryl S-100, indicating that the enzyme was composed of two identical subunits of 16.8 kDa each. N-terminal amino acid sequencing (seven residues) yielded VKAVAVL. Using RACE-PCR, a Cu, Zn-SOD gene was cloned from T. aurantiacus var. levisporus. The sequence was 705 bp and contained a 468 bp ORF encoding a Cu, Zn-SOD of 155 amino acid residues.
authors.affiliation: Department of Environmental Biology, Shandong Agricultural University, Taian, Shandong, China.
authors.forename: Shijin
authors.initials: S
authors.lastname: E
authors.forename: Fangxian
authors.initials: F
authors.lastname: Guo
authors.forename: Shouan
authors.initials: S
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Wang
authors.forename: Duochuan
authors.initials: D
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D001203:Ascomycota / Q000201:enzymology* / Q000235:genetics; D002847:Chromatography, Agarose; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D007700:Kinetics; D008969:Molecular Sequence Data; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D013482:Superoxide Dismutase