title: Comparison of human papilloma virus testing and spectroscopy combined with cervical cytology for the detection of high-grade cervical neoplasia.
abstract: OBJECTIVE
  This study compared the performance of cervical cytology plus human papilloma virus testing (Pap + HPV) or cervical spectroscopy (Pap + CS) for identifying high-grade cervical neoplasia in a high-risk population of women referred for colposcopy.
  
  
  METHODS
  Each of 113 subjects underwent spectroscopy, thin-layer cytology, HPV testing, colposcopy, biopsy when indicated, and/or endocervical curettage. Evaluable data for analysis were collected for 102 of the subjects. Sensitivity and specificity were calculated for both strategies.
  
  
  RESULTS
  Pap + HPV and Pap + CS achieved equivalent sensitivities (95%) for high-grade lesions, with both detecting 17 of 18 histology confirmed cervical intraepithelial neoplasia (CIN) 2+ lesions. Pap + HPV had a specificity of only 27.4% compared with 65.5% for Pap + CS (p < .0001).
  
  
  CONCLUSIONS
  Spectroscopic interrogation of the cervix is equally sensitive and 2-fold more specific than HPV testing when combined with cervical cytology for identifying high-grade cervical neoplasia.
authors.affiliation: Department of Obstetrics and Gynecology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9032, USA. claudia.werner@utsothwestern.edu
authors.forename: Claudia L
authors.initials: CL
authors.lastname: Werner
authors.forename: William F
authors.initials: WF
authors.lastname: Griffith
authors.forename: Raheela
authors.initials: R
authors.lastname: Ashfaq
authors.forename: Diana
authors.initials: D
authors.lastname: Gossett
authors.forename: Edward
authors.initials: E
authors.lastname: Wilkinson
authors.forename: Stephen
authors.initials: S
authors.lastname: Raab
authors.forename: Shabbir
authors.initials: S
authors.lastname: Bambot
authors.forename: David
authors.initials: D
authors.lastname: Mongin
authors.forename: Mark
authors.initials: M
authors.lastname: Faupel
mesh_terms: D000293:Adolescent; D000328:Adult; D001706:Biopsy; D002584:Cervix Uteri / Q000473:pathology / Q000821:virology; D003127:Colposcopy; D004279:DNA, Viral / Q000032:analysis*; D004107:Dilatation and Curettage; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D027383:Papillomaviridae / Q000235:genetics*; D011237:Predictive Value of Tests; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000473:pathology / Q000821:virology; D014626:Vaginal Smears; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis* / Q000473:pathology / Q000821:virology
chemical_list: D004279:DNA, Viral