A simple preparation method for mouse eosinophils and their responses to anti-allergic drugs.
OBJECTIVE
A simple method for preparing mouse eosinophils was established, and the characteristics of the eosinophils were assessed including their responses to anti-allergic drugs.


METHODS
Mouse eosinophils were prepared from peritoneal exudate cells of BALB/c mice primed and boosted with antigen ovalbumin (OVA).


METHODS
Surface phenotypes, migration activities and leukotriene C(4) (LTC(4)) production abilities of these eosinophils were examined. In addition, the effects of anti-allergic drugs, oxatomide and tranilast, on generation of LTC(4) from mouse eosinophils were examined.


RESULTS
Eosinophils of mice boosted with OVA were phenotypically and functionally identical with human eosinophils. Around 1 x 10(7) eosinophils were obtained from mouse peritoneal exudate. It was found that these mouse eosinophils enabled to migrate in response to eotaxin as well as platelet-activating factor (PAF), and generated LTC(4) by IL-5 stimulation. Moreover, it was revealed that clinically used anti-allergic drugs inhibited LTC(4)-production dose-dependently.


CONCLUSIONS
The present study provides a convenient method to obtain fully functional mouse eosinophils that are useful for drug screening and for evaluating implications of eosinophils in allergic responses.
Ebihara
S
S
Japan Tobacco Inc., Central Pharmaceutical Research Institute,1-1 Murasaki-cho, Takatsuki, Osaka 569-1125, Japan. shin.ebihara@ims.jti.co.jp
Kurachi
H
H
Watanabe
Y
Y
D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects; D002469:Cell Separation / Q000379:methods*; D054413:Chemokine CCL11; D018946:Chemokine CCL5 / Q000494:pharmacology; D019742:Chemokines, CC / Q000494:pharmacology; D004804:Eosinophils / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D015848:Interleukin-5 / Q000494:pharmacology; D017997:Leukotriene C4 / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000494:pharmacology; D010641:Phenotype; D010972:Platelet Activating Factor / Q000494:pharmacology
D018926:Anti-Allergic Agents; C515456:CCL11 protein, human; C515457:Ccl11 protein, mouse; D054413:Chemokine CCL11; D018946:Chemokine CCL5; D019742:Chemokines, CC; D015848:Interleukin-5; D010972:Platelet Activating Factor; D017997:Leukotriene C4; D010047:Ovalbumin