Protective effects of amylin on reserpine-induced gastric damage in the rat.
Here we show that the vasoactive peptide amylin protects against reserpine-induced gastric injury in the rat, resulting in lower score of gastric lesions. Hepatocyte growth factor (HGF), its c-Met receptor and cyclooxygenase-2 (COX-2) expression, usually increased in course of reserpine-induced gastric damage, was decreased in rats treated with amylin. Pretreatment with the specific amylin receptor antagonist AC187 abrogated the gastroprotective effects of amylin and restored high expression levels of HGF, c-Met and COX-2. Our data suggest that protective effects of amylin upon the gastric mucosa are specific and eventually involve modulation of HGF, c-Met and COX-2 expression.
Cantarella
Giuseppina
G
Department of Experimental and Clinical Pharmacology, School of Medicine, University of Catania, Italy.
Martinez
Giuseppa
G
Di Benedetto
Giulia
G
Loreto
Carla
C
Musumeci
Giuseppe
G
Prato
Agata
A
Lempereur
Laurence
L
Matera
Mario
M
Amico-Roxas
Matilde
M
Bernardini
Renato
R
Clementi
Giuseppe
G
D000682:Amyloid / Q000494:pharmacology*; D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology; D015153:Blotting, Western; D051546:Cyclooxygenase 2 / Q000378:metabolism; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D058228:Islet Amyloid Polypeptide; D048670:MAP Kinase Kinase 4 / Q000378:metabolism; D008297:Male; D010446:Peptide Fragments / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D019859:Proto-Oncogene Proteins c-met / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D058287:Receptors, Islet Amyloid Polypeptide; D018000:Receptors, Peptide / Q000037:antagonists & inhibitors; D012110:Reserpine / Q000633:toxicity*; D013276:Stomach Ulcer / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*
D000682:Amyloid; D000897:Anti-Ulcer Agents; D058228:Islet Amyloid Polypeptide; D010446:Peptide Fragments; D058287:Receptors, Islet Amyloid Polypeptide; D018000:Receptors, Peptide; C087166:AC 187; D017228:Hepatocyte Growth Factor; D012110:Reserpine; D051546:Cyclooxygenase 2; D019859:Proto-Oncogene Proteins c-met; D048670:MAP Kinase Kinase 4