Investigation on response of the metabolites in tricarboxylic acid cycle of Escherichi coli and Pseudomonas aeruginosa to antibiotic perturbation by capillary electrophoresis.
Metabolomics is a new branch of systems biology exerting its influence in many aspects. In order to appraise the effects of antibiotics on central carbon metabolism, a CE based method was set up. With this platform, we estimated the organic acid metabolite pools' fluctuation of Escherichia coli and Pseudomonas aeruginosa cultured under 11 different antibiotics' stimuli. Multivariate data analysis showed that different antibiotics had clustered distributions for each strain and could be easily distinguished. Genetic, metabolic and antibiotic mechanism differences could also be deduced by the aid of further correlation analysis. For P. aeruginosa, even synergy action amid antibiotics could be ascertained.
Gao
Peng
P
National Chromatographic R & A Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116023, China.
Shi
Chunyun
C
Tian
Jing
J
Shi
Xianzhe
X
Yuan
Kailong
K
Lu
Xin
X
Xu
Guowang
G
D000900:Anti-Bacterial Agents / Q000145:classification / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D002952:Citric Acid Cycle; D019075:Electrophoresis, Capillary / Q000379:methods*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008954:Models, Biological; D015999:Multivariate Analysis; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity
D000900:Anti-Bacterial Agents