Studies on the role of six enzymes in the metabolism of kinetin in mustard aphid, Lipaphis erysimi (Kalt.).
The activity of catalase, glutathione peroxidase, superoxide dismutase, O-demethylase, ATPase and succinate dehydrogenase, belonging to two main classes of detoxification enzymes (i.e. hydrolases and oxido-reductases), mostly involved in metabolism and degradation of xenobiotics in insects, were assessed under the influence of kinetin, a plant growth regulator (PGR). The nymphs (48-52 hr old) of Lipaphis erysimi (Kalt.) were permitted to feed on radish plant, Raphanus sativus L. treated with kinetin (400 ppm) for 13, 25 and 37 hr. It was found that the activity of catalase, glutathione peroxidase and superoxide dismutase increased significantly when compared with the control of the same age group, which indicated that these enzymes might be playing a significant role in the metabolism of kinetin in this insect. The activity of O-demethylase showed an increase up to 25 hr of the treatment but it decreased under prolonged treatment whereas the activity of succinate dehydrogenase fluctuated insignificantly. ATPase showed a decrease in the activity with the treatment suggesting kinetin's interference in synthesis of ATPase.
Rup
Pushpinder J
PJ
Department of Zoology, Guru Nanak Dev University, Amritsar-143 005, India. pushp@tibetan-mastiffs.org
Sohal
S K
SK
Kaur
H
H
D000818:Animals; D001042:Aphids / Q000201:enzymology / Q000378:metabolism*; D004798:Enzymes / Q000378:metabolism*; D007701:Kinetin / Q000378:metabolism*; D031224:Raphanus
D004798:Enzymes; D007701:Kinetin