[Bioinformatic identification and analysis of Schistosoma japonicum adenine phosphoribosyltransferase].
OBJECTIVE
To identify adenine phosphoribosyltransferases in Schistosoma japonicum and analyze their structural features.


METHODS
Based on the accessible transcriptome and proteomic data, the S. japonicum adenine phosphoribosyl transferases were identified using bioinformatics approaches including bi-directional homology comparison, domain search and phylogenetic analysis. Homology modeling was also performed to describe the structural features of the proteins.


CONCLUSIONS
Two homologue sequences of adenine phosphoribosyltransferase were obtained from S. japonicum, and the EST abundance, physico-chemical properties and three-dimensional structures of them were also acquired.
Yang
Zhong
Z
State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China. yangzzh@163.com
Hu
Wei
W
Su
Jin
J
Ma
Li
L
Li
Yi-xue
YX
Feng
Zheng
Z
Wei
Dong-zhi
DZ
D000228:Adenine Phosphoribosyltransferase / Q000737:chemistry / Q000235:genetics*; D000818:Animals; D019295:Computational Biology / Q000379:methods*; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics*; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics; D008958:Models, Molecular; D010802:Phylogeny; D011487:Protein Conformation; D012549:Schistosoma japonicum / Q000201:enzymology*
D015801:Helminth Proteins; D007527:Isoenzymes; D000228:Adenine Phosphoribosyltransferase