title: Herpes simplex virus type 1 induces CD83 degradation in mature dendritic cells with immediate-early kinetics via the cellular proteasome.
abstract: Mature dendritic cells (DCs) are the most potent antigen-presenting cells within the human immune system. However, Herpes simplex virus type 1 (HSV-1) is able to interfere with DC biology and to establish latency in infected individuals. In this study, we provide new insights into the mechanism by which HSV-1 disarms DCs by the manipulation of CD83, a functionally important molecule for DC activation. Fluorescence-activated cell sorter (FACS) analyses revealed a rapid downmodulation of CD83 surface expression within 6 to 8 h after HSV-1 infection, in a manner strictly dependent on viral gene expression. Soluble CD83 enzyme-linked immunosorbent assays, together with Western blot analysis, demonstrated that CD83 rapidly disappears from the cell surface after contact with HSV-1 by a mechanism that involves protein degradation rather than shedding of CD83 from the cell surface into the medium. Infection experiments with an ICP0 deletion mutant demonstrated an important role for this viral immediate-early protein during CD83 degradation, since this particular mutant strain leads to strongly reduced CD83 degradation. This hypothesis was further strengthened by cotransfection of plasmids expressing CD83 and ICP0 into 293T cells, which led to significantly reduced accumulation of CD83. In strong contrast, transfection of plasmids expressing CD83 and a mutant ICP0 defective in its RING finger-mediated E3 ubiquitin ligase function did not reduce CD83 expression. Inhibition of the proteasome, the cellular protein degradation machinery, almost completely restored CD83 surface expression during HSV-1 infection, indicating that proteasome-mediated degradation and HSV-1 ICP0 play crucial roles in this novel viral immune escape mechanism.
authors.affiliation: Department of Dermatology, University Hospital Erlangen, Hartmannstrasse 14, D-91052 Erlangen, Germany.
authors.forename: Mirko
authors.initials: M
authors.lastname: Kummer
authors.forename: Nadine M
authors.initials: NM
authors.lastname: Turza
authors.forename: Petra
authors.initials: P
authors.lastname: Muhl-Zurbes
authors.forename: Matthias
authors.initials: M
authors.lastname: Lechmann
authors.forename: Chris
authors.initials: C
authors.lastname: Boutell
authors.forename: Robert S
authors.initials: RS
authors.lastname: Coffin
authors.forename: Roger D
authors.initials: RD
authors.lastname: Everett
authors.forename: Alexander
authors.initials: A
authors.lastname: Steinkasserer
authors.forename: Alexander T
authors.initials: AT
authors.lastname: Prechtel
mesh_terms: D015703:Antigens, CD / Q000096:biosynthesis*; D002460:Cell Line; D002469:Cell Separation; D003713:Dendritic Cells / Q000166:cytology*; D015536:Down-Regulation; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006561:Herpes Simplex / Q000276:immunology; D018259:Herpesvirus 1, Human / Q000378:metabolism*; D006801:Humans; D017874:Immediate-Early Proteins / Q000378:metabolism; D007136:Immunoglobulins / Q000096:biosynthesis*; D007700:Kinetics; D007963:Leukocytes, Mononuclear / Q000821:virology; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D014162:Transfection; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism; D000097802:CD83 Antigen
chemical_list: D015703:Antigens, CD; D017874:Immediate-Early Proteins; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D046988:Proteasome Endopeptidase Complex; D044767:Ubiquitin-Protein Ligases; D000097802:CD83 Antigen; C051804:Vmw110 protein, Human herpesvirus 1