[Radiosensitivity of nasopharyngeal carcinoma in relation to cell cycle synchronization effect of tumor necrosis factor alpha].
OBJECTIVE
To investigate the effect of tumor necrosis factor alpha (TNFalpha) on radiosensitivity of nasopharyngeal carcinoma (NPC) in relation to TNFalpha-induced cell cycle synchronization.


METHODS
The radio-resistance of a NPC cell line subclone CNE-2Z-S1 was verified by in vivo experiments and flow cytometry was performed to evaluate cell cycle synchronization in TNFalpha-treated CNE-2Z-S1 cells. The radiosensitivity of the cell synchronized CNE-2Z-S1 cells was determined by clone formation in vitro and in vivo experiment in nude mice.


RESULTS
TNFalpha was capable of inducing cell cycle arrest and synchronization of CNE-2Z-S1 cells. Pretreatment with TNFalpha remarkably enhanced the radiosensitivity of CNE-2Z-S1 in vitro, and in vivo experiments with nude mice also suggested the role of TNFalpha in enhancing the radiosensitivity of NPC.


CONCLUSIONS
TNFalpha can enhance the radiosensitivity of NPC cells by inducing cell cycle synchronization.
Yuan
Jian
J
Department of Pathology, Guangdong Pharmacy College, Guangzhou 510224, China. janeyuan9909@sina.com
Sun
Ning
N
Wang
Li-jing
LJ
Tian
Wei
W
Zhou
Xiao-ming
XM
Chen
Dong-bo
DB
Gu
Hong-biao
HB
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects* / Q000528:radiation effects; D045744:Cell Line, Tumor; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009303:Nasopharyngeal Neoplasms / Q000188:drug therapy* / Q000473:pathology / Q000532:radiotherapy; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D023041:Xenograft Model Antitumor Assays
D011838:Radiation-Sensitizing Agents; D014409:Tumor Necrosis Factor-alpha