title: Antibacterial activity of bovine lactoferrin hydrolysate against mastitis pathogens and its effect on superoxide production of bovine neutrophils.
abstract: Antibacterial activity of bovine lactoferrin hydrolysates (LFH) on microorganisms isolated from bovine mastitis, and superoxide (O(2)(-)) production of bovine neutrophils were evaluated. Antibacterial effects of LFH were measured in vitro against Staphylococcus aureus, coagulase-negative staphylococci, Streptococci, Enterococci, Escherichia coli, Klebsiella pneumoniae, yeast-like fungi and Prototheca zopfii isolated from clinical cases of bovine mastitis. To compare susceptibilities against LFH, minimal inhibitory concentration (MIC) values were determined by a micro-plate assay method. Most organisms were sensitive to LFH. Prototheca zopfii was highly sensitive to LFH; the growth of the microorganism was inhibited completely even at 1 mug/ml. Staphylococcus aureus and Escherichia coli were resistant to LFH. The production of O(2)(-) by bovine neutrophils was used to evaluate the effect of LFH administration on functional activity. Increase in O(2)(-) production by bovine neutrophils occurred upon addition of LFH to neutrophils. These results demonstrate that LFH possesses antibacterial activity against pathogens that cause mastitis and activates neutrophil superoxide production.
authors: Seibu Veterinary Clinical Center, Tokachi NOSAI, Shimizu 089-0103, Japan. kawai@netbeet.ne.jp
authors: K
authors: K
authors: Kawai
authors: K
authors: K
authors: Shimazaki
authors: H
authors: H
authors: Higuchi
authors: H
authors: H
authors: Nagahata
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D005260:Female; D005658:Fungi / Q000187:drug effects; D007781:Lactoferrin / Q000627:therapeutic use*; D008414:Mastitis, Bovine / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D008892:Milk / Q000382:microbiology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D011492:Protein Hydrolysates; D013481:Superoxides / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D011492:Protein Hydrolysates; D013481:Superoxides; D007781:Lactoferrin