title: RGD-dependent mechanotransduction of suspension cultured Taxus cell in response to shear stress.
abstract: Plant cells cultured in bioreactors are strongly influenced by mechanical forces. However, the molecular mechanism of plant cell mechanoreception has maintained unclear. In animal cells, the Arg-Gly-Asp (RGD) motif can be found in proteins of the extracellular matrix. Integrins link the intracellular cytoskeleton of cells with the extracellular matrix by recognizing this RGD motif. Integrin has been demonstrated to function as an apparatus not only for adhesion but also for mechanotransduction. In plant cells, the molecules that mediate the structural continuity between wall and membrane are unknown. Here, we found that synthetic RGD peptide could dramatically reduce the level of phosphorylation of MAPK-like cascades that are activated by shear stress and reduce the alkalinization response, production of reactive oxygen species (ROS) and accumulation of phenolics by Taxus cuspidata cells during shear stress. These results implicate that a RGD recognition system may exist in Taxus cells and play an important role in signal transduction of shear stress. Although the Arabidopsis genome database shows that the plant seems to lack a homologue of animal integrin, plant cells may use other RGD-binding proteins to recognize the RGD motif. The correlative mechanism is discussed.
authors: Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China.
authors: Hong
authors: H
authors: Gao
authors: Yan-Wen
authors: YW
authors: Gong
authors: Ying-Jin
authors: YJ
authors: Yuan
mesh_terms: D015153:Blotting, Western; D002478:Cells, Cultured; D002853:Chromatography, Liquid; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009842:Oligopeptides / Q000494:pharmacology*; D010636:Phenols / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D013314:Stress, Mechanical; D053719:Tandem Mass Spectrometry; D020946:Taxus / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism
chemical_list: D009842:Oligopeptides; D010636:Phenols; D017382:Reactive Oxygen Species; C047981:arginyl-glycyl-aspartic acid; D020928:Mitogen-Activated Protein Kinases