Self-aggregation--an intrinsic property of G(M1) in lipid bilayers.
We demonstrate that the ganglioside G(M1) in lipid bilayers of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) exhibits a non-uniform lateral distribution, i.e., enriched regions of GM(1) molecules are formed, which is an argument in favour of self-aggregation of G(M1) being an intrinsic property of G(M1) ganglioside. This was concluded from energy transfer/migration studies of BODIPY-labelled gangliosides by means of time-resolved fluorescence lifetime and depolarization experiments. Three fluorophore-labelled gangliosides were synthesized to include either of two spectroscopically different BODIPY groups. These were specifically localized either in the polar headgroup region or in the non-polar region of the lipid bilayer. An eventual ganglioside-ganglioside affinity/aggregation induced by the BODIPY groups was experimentally excluded, which suggests their use in examining the influence of G(M1) in more complex systems.
Marushchak
Denys
D
Department of Chemistry, Biophysical Chemistry, Umeå University, Umeå, Sweden.
Gretskaya
Natalia
N
Mikhalyov
Ilya
I
Johansson
Lennart B-A
LB
D004735:Energy Transfer; D005677:G(M1) Ganglioside / Q000737:chemistry*; D008051:Lipid Bilayers / Q000138:chemical synthesis / Q000737:chemistry*; D008954:Models, Biological; D013499:Surface Properties; D053835:Unilamellar Liposomes / Q000138:chemical synthesis / Q000737:chemistry
D008051:Lipid Bilayers; D053835:Unilamellar Liposomes; D005677:G(M1) Ganglioside