A comparative study of micellar and microemulsion EKC for the analysis of benzoylurea insecticides and their analogs.
An investigation of the basic factors which govern the microemulsion EKC (MEEKC) and MEKC for the separation of four benzoylurea (BU) insecticides and their four analogs was carried out. In MEEKC, the separation of eight BU compounds was optimized by changing the microemulsion composition, such as concentration of SDS, octane, n-butanol, and isopropanol percentages, as well as capillary temperature. Separation optimization was also carried out for MEKC, showing that ACN and a high level of another additive gamma-CD were needed to achieve effective separation of these analytes. Although separation with baseline resolution was achieved by either MEEKC or MEKC methods, the separation selectivity resulting from the proposed MEEKC method was completely different from that of MEKC. In addition, analytical time in MEEKC was longer than that in MEKC, but in view of theoretical plate numbers, detection limits, and reproducibility, both methods were effective for the analysis of BU insecticides and their analogs.
Yang
Xia
X
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing, P. R. China.
Xia
Yan
Y
Tao
Chuanjiang
C
Liao
Yiping
Y
Zuo
Yumin
Y
Liu
Huwei
H
D001549:Benzamides / Q000032:analysis; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004132:Diflubenzuron / Q000032:analysis; D004655:Emulsions / Q000737:chemistry; D007306:Insecticides / Q000032:analysis*; D010671:Phenylurea Compounds / Q000032:analysis*; D015203:Reproducibility of Results; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry; D013696:Temperature; D005466:Fluorocarbons
D001549:Benzamides; D004132:Diflubenzuron; D004655:Emulsions; D007306:Insecticides; D010671:Phenylurea Compounds; D012967:Sodium Dodecyl Sulfate; D013501:Surface-Active Agents; C058039:hexafluoron; D005466:Fluorocarbons; C067660:flufenoxuron