Porcine follicular fluids: comparison of solid-phase extraction and matrix solid-phase dispersion for the GC-MS determination of hormones during follicular growth.
The capabilities of solid-phase extraction (SPE) and matrix solid-phase dispersion (MSPD) for the determination of the hormones 17beta-estradiol, 2-hydroxyestradiol, 4-hydroxyestradiol and 2-methoxyestradiol by gas chromatography-mass spectrometry (GC-MS) in a very complex matrix like porcine follicular fluids were compared, thus proving the highest effectiveness of the SPE technique. Validation was carried out in terms of limit of quantitation (LOQ), precision, accuracy, recovery and stability. LOQ values in the low microg kg(-1) were achieved, with all the other parameters satisfying the acceptance criteria for the validation of bioanalytical methods. The applicability of the method to the determination of the hormones in porcine follicular fluids was demonstrated, thus allowing to observe an increase of the concentration of the hormones during the follicular growth.
Bianchi
F
F
Dipartimento di Chimica Generale ed Inorganica, Chimica Analitica, Chimica Fisica, Università degli Studi di Parma, Viale Usberti 17/A, 43100 Parma, Italy. federica.bianchi@unipr.it
Careri
M
M
Mangia
A
A
Musci
M
M
Santini
S E
SE
Basini
G
G
D000077584:2-Methoxyestradiol; D000818:Animals; D004958:Estradiol / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry; D002393:Estrogens, Catechol; D005260:Female; D015571:Follicular Fluid / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006728:Hormones / Q000032:analysis* / Q000737:chemistry; D015394:Molecular Structure; D006080:Ovarian Follicle / Q000737:chemistry / Q000254:growth & development*; D010053:Ovary / Q000737:chemistry; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D013552:Swine
D002393:Estrogens, Catechol; D006728:Hormones; D004958:Estradiol; D000077584:2-Methoxyestradiol; C014036:4-hydroxyestradiol