[Influence of adenovirus transfection on the maturation characteristics of human immature dendritic cells].
OBJECTIVE
To observe the changes in the phenotype characteristics and immune function after transfection of cord blood derived immature dendritic cells( imDC) with Adeasy-EGFP adenovirus vector, and to explore the function of IL-10 in inhibition of imDC maturation.


METHODS
Immature dendritic cells were generated from human cord blood(CB) monocyte cultured with rhGM-CSF and rhIL-4. The recombinant adenovirus vector AdEASY-EGFP was transduced into immature dendritic cells on the third day with or without adding IL-10. The expression of cell maturation marker CD83, CD86 and HLA-DR were determined with flow cytometry. Allogeneic mixed leukocyte reaction( MLR) was used to examine the imDC's ability to promote T cell proliferation.


RESULTS
The expression of surface maturation markers of imDC after transfection with adenovirus were significantly up-regulated ( CD86:46+/-10; CD83: 38 +/- 7; HLA-DR: 82 + 12) , and its ability to promote T cell proliferation was also obviously increased( SI > 2. 0). However, the expression of surface maturation markers of imDC after IL-10 treatment had lower mature phenotypes expression after transduction (CD86:8 +/- 5; CD83: 9 +/- 3; HLA-DR:63 +/- 12), and T cell stimulating ability was decreased comparing with adenovirus transfection groups.


CONCLUSIONS
Adenovirus can be transduced into imDC with high efficiency, but transfection itself can promote imDC's maturation. IL-10 treatment can inhibit the tendency to maturation stimulated by adenovirus transduction efficiently.
Wang
Yong-quan
YQ
Institute of Burn Research, Southwest Hospital, State Key Laboratry of Trauma, Burns and Combine Injury, the Third Military Medical University, Chongqing 400038, PR China.
Peng
Yi-zhi
YZ
Wang
Qiang
Q
Wang
Yi-tao
YT
You
Bo
B
D000256:Adenoviridae / Q000235:genetics; D002454:Cell Differentiation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology*; D005822:Genetic Vectors; D006801:Humans; D016753:Interleukin-10 / Q000494:pharmacology; D013601:T-Lymphocytes / Q000378:metabolism; D014162:Transfection
D016753:Interleukin-10