title: Structural characterisation of unsaturated bacterial hopanoids by atmospheric pressure chemical ionisation liquid chromatography/ion trap mass spectrometry.
abstract: The production of bacteriohopanepolyols (BHPs) is widespread in many different groups of prokaryotes; however, unsaturated components are less common except amongst the acetic acid bacteria. Here we describe the characterisation of mono- (Delta(6) or Delta(11)) and diunsaturated (Delta(6,11)) bacteriohopanetetrols isolated from the acetic acid bacterium Gluconacetobacter xylinus (formerly Acetobacter aceti ssp. xylinum) by atmospheric pressure chemical ionisation ion trap mass spectrometry (APCI-MS(n)). APCI-MS(2) spectra are compared with equivalent electron ionisation (EI) spectra and differences in fragmentation pathways are discussed. Having established characteristic spectral features for a range of unsaturated BHPs we now have the ability to rapidly detect the presence of unsaturated BHPs in both natural environmental samples (soils, sediments, water columns) as well as in microbial cultures.
authors: School of Civil Engineering and Geosciences, Devonshire Building, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK. h.m.talbot@ncl.ac.uk
authors: Helen M
authors: HM
authors: Talbot
authors: Michel
authors: M
authors: Rohmer
authors: Paul
authors: P
authors: Farrimond
mesh_terms: D000091:Acetobacter / Q000737:chemistry; D000107:Acetylation; D001419:Bacteria / Q000737:chemistry*; D002853:Chromatography, Liquid; D041821:Gluconacetobacter / Q000737:chemistry; D012015:Reference Standards; D012241:Rhodopseudomonas / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D014315:Triterpenes / Q000737:chemistry*
chemical_list: D014315:Triterpenes