Inhibition of photophosphorylation and electron transport chain in thylakoids by lasiodiplodin, a natural product from Botryosphaeria rhodina.
Four natural products were isolated from the fungus Botryosphaeria rhodina, and their effects on photosynthesis were tested. Only lasiodiplodin (1) inhibited ATP synthesis and electron flow from water to methylviologen; therefore, it acts as a Hill reaction inhibitor in freshly lysed spinach thylakoids. Photosystem I and II and partial reactions as well as ATPase were measured in the presence of 1. Three new different sites of 1 interaction and inhibition were found: one at CF1, the second in the water-splitting enzyme, and the third at the electron-transfer path between P680 and QA; these targets are different from that of the synthetic herbicides present. Electron transport chain inhibition by 1 was corroborated by fluorescence induction kinetics studies.
Veiga
Thiago A M
TA
Departamento de Bioquímica, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510 México DF, Mexico.
Silva
Sebastião C
SC
Francisco
Archundia-Camacho
AC
Filho
Edson R
ER
Vieira
Paulo C
PC
Fernandes
João B
JB
Silva
Maria F G F
MF
Müller
Manfred W
MW
Lotina-Hennsen
Blas
B
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors; D000255:Adenosine Triphosphate / Q000096:biosynthesis; D001203:Ascomycota / Q000737:chemistry*; D004579:Electron Transport / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D010785:Photophosphorylation / Q000187:drug effects*; D018515:Plant Leaves / Q000648:ultrastructure; D018724:Spinacia oleracea / Q000648:ultrastructure; D020524:Thylakoids / Q000187:drug effects* / Q000378:metabolism*; D015025:Zearalenone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology
D004791:Enzyme Inhibitors; C417552:lasiodiplodin; D015025:Zearalenone; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases