title: Genomic analysis of genetic markers associated with inherited cardiomyopathy (round heart disease) in the turkey (Meleagris gallopavo).
abstract: In turkeys, spontaneous cardiomyopathy or round heart (RH) disease is characterised by dilated ventricles and cardiac muscle hypertrophy. Although the aetiology of RH is still unknown, the disease can have a significant economic impact on turkey producers. In an initial attempt to identify genomic regions associated with RH, we utilised the chicken genome sequence to target short DNA sequences (sequence-characterised amplified regions, SCARs) identified in previous studies that had significant differences in frequency distribution between RH+ and RH- turkeys. SCARs were comparatively aligned with the chicken whole-genome sequence to identify flanking regions for primer design. Primers from 32 alignments were tested and target sequences were successfully amplified for 30 loci (94%). Comparative re-sequencing identified putative SNPs in 20 of the 30 loci (67%). Genetically informative SNPs at 16 loci were genotyped in the UMN/NTBF turkey mapping population. As a result of this study, 34 markers were placed on the turkey/chicken comparative map and 15 markers were added to the turkey genetic linkage map. The position of these markers relative to cardiac-related genes is presented. In addition, analysis of genotypes at 109 microsatellite loci presumed to flank the SCAR sequences in the turkey genome identified four significant associations with RH.
authors: Department of Veterinary and Biomedical Sciences, University of Minnesota, St Paul, MN 55108, USA. reedx054@umn.edu
authors: K M
authors: KM
authors: Reed
authors: K M
authors: KM
authors: Mendoza
authors: G R
authors: GR
authors: Hu
authors: L R
authors: LR
authors: Sullivan
authors: M W
authors: MW
authors: Grace
authors: L D
authors: LD
authors: Chaves
authors: D L
authors: DL
authors: Kooyman
mesh_terms: D000818:Animals; D001483:Base Sequence; D009202:Cardiomyopathies / Q000235:genetics / Q000662:veterinary*; D017931:DNA Primers; D005819:Genetic Markers / Q000235:genetics*; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011201:Poultry Diseases / Q000235:genetics*; D021101:Radiation Hybrid Mapping; D017422:Sequence Analysis, DNA; D014422:Turkeys
chemical_list: D017931:DNA Primers; D005819:Genetic Markers