title: Profile of cytokine gene polymorphisms in Iranian multiple sclerosis patients.
abstract: BACKGROUND
  Cytokine gene polymorphisms have been extensively studied in association with different diseases. The role of cytokine gene polymorphisms in multiple sclerosis (MS), as a chronic immune-mediated neurodegenerative disease, has been previously reported.
  
  
  METHODS
  DNA samples were collected from 44 patients with relapsing-remitting multiple sclerosis (RRMS) and 140 unrelated healthy subjects. All participants in this study were matched for ethnicity. Cytokine gene SNPs were determined using the PCR-SSP method.
  
  
  RESULTS
  and discussion We found no significant differences between MS patients and controls in most of the studied cytokine genes. Remarkable results were obtained for IL-2 GG-330 genotype (P =0.06), IL-6 C-174 allele (P =0.06), CG and GG genotypes (P <0.001), and GG (P =0.02) and CG (P <0.001) haplotypes, and TNF-alpha A-238 allele (P <0.001), GG (P =0.003) and GA (P <0.001) haplotypes. These results suggest that polymorphic variations of these pro-inflammatory cytokines play an important role in susceptibility to MS.
authors: Immunogenetic Laboratory, Department of Immunology, Medical School, Tehran University of Medical Sciences, Tehran, Iran. amirzargar_ali@yahoo.com
authors: A
authors: A
authors: Amirzargar
authors: F
authors: F
authors: Khosravi
authors: S
authors: S
authors: Dianat
authors: F
authors: F
authors: Hushmand
authors: P
authors: P
authors: Maryousef
authors: A R
authors: AR
authors: Foroushani
authors: J
authors: J
authors: Lotfi
authors: B
authors: B
authors: Nikbin
mesh_terms: D000293:Adolescent; D000328:Adult; D016207:Cytokines / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D007376:Interleukin-2 / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D007492:Iran; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000235:genetics* / Q000276:immunology; D020641:Polymorphism, Single Nucleotide; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D016207:Cytokines; D007376:Interleukin-2; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha