title: IQGAP1 regulates adult neural progenitors in vivo and vascular endothelial growth factor-triggered neural progenitor migration in vitro.
abstract: In the germinative zone of the adult rodent brain, neural progenitors migrate into niches delimited by astrocyte processes and differentiate into neuronal precursors. In the present study, we report a modulating role for the scaffolding protein IQGAP1 in neural progenitor migration. We have identified IQGAP1 as a new marker of amplifying neural progenitor and neuronal precursor cells of the subventricular zone (SVZ) and the rostral migratory stream (RMS) in the adult mouse brain. To determine functions for IQGAP1 in neural progenitors, we compared the properties of neural progenitor cells from wild-type and Iqgap1-null mutant mice in vivo and in vitro. The in vivo studies reveal a delay in the transition of de novo neural progenitors into neuronal precursor cells in Iqgap1-null mice. In vitro, we demonstrated that IQGAP1 acts as a downstream effector in the vascular endothelial growth factor (VEGF)-dependent migratory response of neural progenitors that also impacts on their neuronal differentiation. The Rho-family GTPases cdc42/Rac1 and Lis1 are major partners of IQGAP1 in this migratory process. Finally, astrocytes of the neurogenic SVZ and RMS are shown to express VEGF. We propose that VEGF synthesized by astrocytes could be involved in the guidance of neural progenitors to neurogenic niches and that IQGAP1 is an effector of the VEGF-dependent migratory signal.
authors: Institut National de la Santé et de la Recherche Médicale, Unité 873, Laboratoire Transduction du Signal, F-38054 Grenoble, France.
authors: Laurent
authors: L
authors: Balenci
authors: Yasmina
authors: Y
authors: Saoudi
authors: Didier
authors: D
authors: Grunwald
authors: Jean Christophe
authors: JC
authors: Deloulme
authors: Alexandre
authors: A
authors: Bouron
authors: André
authors: A
authors: Bernards
authors: Jacques
authors: J
authors: Baudier
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology* / Q000502:physiology*; D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism; D002450:Cell Communication / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D002552:Cerebral Ventricles / Q000166:cytology; D066298:In Vitro Techniques; D051379:Mice; D018345:Mice, Knockout; D009474:Neurons / Q000166:cytology*; D009479:Neuropeptides / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism / Q000494:pharmacology; D020764:cdc42 GTP-Binding Protein / Q000378:metabolism; D020744:rac GTP-Binding Proteins / Q000378:metabolism; D020830:rac1 GTP-Binding Protein; D020703:ras GTPase-Activating Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C101203:IQ motif containing GTPase activating protein 1; D009479:Neuropeptides; C484602:Rac1 protein, mouse; D042461:Vascular Endothelial Growth Factor A; D020703:ras GTPase-Activating Proteins; D020764:cdc42 GTP-Binding Protein; D020744:rac GTP-Binding Proteins; D020830:rac1 GTP-Binding Protein