Comparative analysis of the catechol 2,3-dioxygenase gene locus in thermoacidophilic archaeon Sulfolobus solfataricus strain 98/2.
A catechol 2,3-dioxygenase (C23O) gene was found from Sulfolobus solfataricus strain 98/2. Heterologous thermophilic C23O expressed in Escherichia coli showed the highest activity against catechol and 4-chlorocatechol, and at neutral pH. The C23O gene located with a putative multicomponent monooxygenase (MM) gene cluster that exactly matched with the homologous region of S. solfataricus strain P2. Primary sequence comparison identified an insertion sequence (IS) element inserted into a putative MM protein A N-terminal fragment gene in strain 98/2. Both ends of the transposase gene in the IS element, ISC1234, were flanked by 19 bp inverted repeat and 4 bp direct repeat sequences which are typical features of mobile elements. Our analysis and the two geographically distant origins of strains 98/2 and P2 (USA and Italy, respectively) suggest that the two strains have evolved from a common ancestor.
Chae
Jong-Chan
JC
Biotechnology Center for Agriculture and Environment, School of Environmental and Biological Sciences, Rutgers University, New Brunswick, NJ 08901-8520, USA.
Kim
Eungbin
E
Bini
Elisabetta
E
Zylstra
Gerben J
GJ
D019843:Archaeal Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D050557:Catechol 2,3-Dioxygenase / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004251:DNA Transposable Elements / Q000235:genetics; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004795:Enzyme Stability; D020745:Genome, Archaeal; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D005810:Multigene Family; D016254:Mutagenesis, Insertional; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013379:Substrate Specificity; D048229:Sulfolobus solfataricus / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D013696:Temperature
D019843:Archaeal Proteins; D004251:DNA Transposable Elements; D019641:DNA, Archaeal; D050557:Catechol 2,3-Dioxygenase