title: Suppression of ochratoxin biosynthesis by naturally occurring alkaloids.
abstract: The effects of four alkaloids on the biosynthesis of ochratoxin A (OTA), ochratoxin B (OTB) and citrinin were examined on four OTA-producing aspergilli: Aspergillus auricomus, A. sclerotiorum and two isolates of A. alliaceus. Piperine and piperlongumine, natural alkaloids of Piper longum, significantly inhibited OTA production at 0.001% (w/v) for all aspergilli examined. Piperine and piperlongumine affected the polyketide synthesis step of OTA production and inhibited production of citrinin. Curcumin, a constituent of tumeric, completely inhibited mycelial growth of A. alliaceus isolate 791 at 0.1% (w/v) and decreased OTA production by approximately 70% at 0.01% (w/v). Sesamin, a constituent of sesame oil, inhibited OTA and OTB production by 60 and 45%, respectively, at 0.1% (w/v), showing its effect was on chloroperoxidase and polyketide synthase activity. The potential advantage of these natural products to reduce ochratoxin contamination of agricultural commodities is discussed.
authors: Research Station, Nanotoxtech Co. Ltd., Bundang, Sungnam, South Korea. selpest@hanmail.net
authors: S E
authors: SE
authors: Lee
authors: B S
authors: BS
authors: Park
authors: P
authors: P
authors: Bayman
authors: J L
authors: JL
authors: Baker
authors: W S
authors: WS
authors: Choi
authors: B C
authors: BC
authors: Campbell
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D001230:Aspergillus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D052117:Benzodioxoles / Q000494:pharmacology; D002273:Carcinogens / Q000378:metabolism; D002953:Citrinin / Q000096:biosynthesis; D003470:Culture Media; D030024:Curcuma / Q000737:chemistry; D003474:Curcumin / Q000494:pharmacology; D004148:Dioxolanes / Q000494:pharmacology; D004149:Dioxoles / Q000494:pharmacology; D005506:Food Contamination / Q000032:analysis; D017705:Lignans / Q000494:pharmacology; D025282:Mycelium / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D009183:Mycotoxins / Q000096:biosynthesis*; D009793:Ochratoxins / Q000096:biosynthesis; D031701:Piper / Q000737:chemistry; D010880:Piperidines / Q000494:pharmacology; D053284:Polyunsaturated Alkamides / Q000494:pharmacology; D012715:Sesame Oil / Q000737:chemistry; D010881:Piperidones
chemical_list: D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D052117:Benzodioxoles; D002273:Carcinogens; D002953:Citrinin; D003470:Culture Media; D003474:Curcumin; D004148:Dioxolanes; D004149:Dioxoles; D017705:Lignans; D009183:Mycotoxins; D009793:Ochratoxins; D010880:Piperidines; D053284:Polyunsaturated Alkamides; D012715:Sesame Oil; C025589:ochratoxin A; C045354:ochratoxin B; C498077:piperlongumine; D010881:Piperidones; C054125:sesamin; C008922:piperine