MMP-2, MMP-9 and TIMP-2 gene polymorphisms in Chinese patients with generalized aggressive periodontitis.
BACKGROUND
Aggressive periodontitis (AgP) has a genetic basis. It has been reported that the functional gene polymorphisms of matrix metalloproteinase (MMP)-2, MMP-9 and tissue inhibitor of metalloproteinase-2 (TIMP-2) alter their expressions in transcriptional level and they are involved in the tissue destruction of periodontitis. The study was carried out to analyse the association of functional polymorphisms in MMP-2, MMP-9 and TIMP-2 with generalized AgP (G-AgP) in a Chinese population.


METHODS
The study population consisted of 79 Chinese patients with G-AgP and 128 healthy controls. DNA was obtained from oral mucosa swab samples. MMP-2 genotypes were determined by PCR-based denaturing high-performance liquid chromatography analysis while MMP-9 and TIMP-2 genotypes were identified by a PCR-based restriction fragment length polymorphism. Chi2 test after Yates' correction was used to investigate the possible association of the genotypes with the G-AgP.


RESULTS
Although gene polymorphisms for MMP-2 and MMP-9 did not show any association with the G-AgP, the analysis of the TIMP-2 -418G to C gene polymorphism revealed significant differences between the patients and controls. Compared with controls, a significant increasing trend of TIMP-2 -418C carrier in the G-AgP patients occurred (p=0.013).


CONCLUSIONS
It is suggested that the TIMP2 -418G to C gene polymorphism is associated with G-AgP in the Chinese subjects.
Chen
Dong
D
Department of Periodontology and Oral Medicine, School of Stomatology, China.
Wang
Qi
Q
Ma
Zhi-Wei
ZW
Chen
Fa-Ming
FM
Chen
Yue
Y
Xie
Guang-Yuan
GY
Wang
Qin-Tao
QT
Wu
Zhi-Fen
ZF
D000328:Adult; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D002681:China; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics*; D061965:Matrix Metalloproteinase Inhibitors; D009061:Mouth Mucosa / Q000201:enzymology; D010518:Periodontitis / Q000201:enzymology* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000235:genetics*
D061965:Matrix Metalloproteinase Inhibitors; D019716:Tissue Inhibitor of Metalloproteinase-2; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9