Development of a novel glue consisting of naturally-derived biomolecules: citric acid and human serum albumin.
A novel glue consisting of human serum albumin (HSA) and citric acid derivative (CAD) was developed where the glue is named as CAD-A glue. In this adhesive, CAD works as a crosslinking reagent of HSA. For preparing crosslinking reagent CAD, using citric acid as a starting material, three carboxyl groups of a citric acid were modified with N-hydroxysuccinimide in the presence of 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride. From 1H-NMR spectrum, CAD with three active ester groups in a molecule was successfully synthesized with a high yield (more than 80%). The boding time of CAD-A glue to collagen-based casing was saturated within 15 minutes. The bonding strength of this glue to collagen-based casings increased with increasing of HSA concentration. The maximum bonding strength of CAD-A glue was a slightly low level compared to the bonding strength of cyanoacrylate adhesive and was 9 times higher than that of fibrin glue. The CAD-A glue showed excellent biocompatibility and high ability of wound closure similar to that of cyanoacrylate-based adhesive when glues were applied to the mouse skin. These results suggested that this developed adhesive had both tissue compatibility and bonding strength for use in clinical field.
Taguchi
Tetsushi
T
Biomaterials Center National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan.
Saito
Hirofumi
H
Iwasashi
Masashi
M
Sakane
Masataka
M
Kakinoki
Sachiro
S
Ochiai
Naoyuki
N
Tanaka
Junzo
J
D000269:Adhesives / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D019343:Citric Acid / Q000737:chemistry; D003487:Cyanoacrylates; D015718:Fibrin Tissue Adhesive; D008422:Materials Testing; D051379:Mice; D036103:Nanotechnology; D012709:Serum Albumin / Q000737:chemistry; D012867:Skin / Q000293:injuries / Q000473:pathology; D014014:Tissue Adhesives / Q000737:chemistry*
D000269:Adhesives; D001672:Biocompatible Materials; D003487:Cyanoacrylates; D015718:Fibrin Tissue Adhesive; D012709:Serum Albumin; D014014:Tissue Adhesives; D019343:Citric Acid