[Decompactization of chromosome 1 in the artificially decondensed human sperm nuclei: overall topology and non-random location of chromosome bends].
We have used FISH with microdissected chromosome arm-specific DNA probes that allow direct visualization of chromosome 1 folding in human sperm nuclei. We described the overall topology and the mode of packaging of sperm chromosome 1 and thus established new elements of ordered genome architecture in these specialized cells.
Mudrak
O S
OS
Tomilin
N V
NV
Zelenskiĭ
A O
AO
D002467:Cell Nucleus / Q000235:genetics*; D002878:Chromosomes, Human, Pair 1 / Q000378:metabolism* / Q000648:ultrastructure; D016678:Genome; D006801:Humans; D008297:Male; D008856:Microscopy, Fluorescence; D013094:Spermatozoa / Q000166:cytology*