title: Trauma-associated inflammatory response impairs embryonic stem cell survival and integration after implantation into injured rat brain.
abstract: Pluripotent embryonic stem cells were shown to survive and differentiate into mature neuronal cells after implantation in experimental models of Parkinson disease and cerebral ischemia. Embryonic stem cell transplantation has also been proposed as a potential therapy for cerebral trauma, characteristic of massive loss of multiple cell types due to primary insult and secondary sequelae. Green fluorescent protein (GFP)-transfected murine embryonic stem cells were implanted into the ipsi or contralateral cortex of male Sprague-Dawley rats 72 h after fluid-percussion injury. Animals were sacrificed at day 5 or week 7 postimplantation. Brain sections were examined using conventional and fluorescent double-labelling immunohistochemistry. Five days after implantation, clusters of GFP-positive cells undergoing partial differentiation along neuronal pathway, were detected at the implantation site. However, after 7 weeks, only a few GFP-positive cells were found, indicating an extensive loss of stem cells during this time period. For the first time, we proved the observed cell loss to be mediated via phagocytosis of implanted cells by activated macrophages. Cerebral trauma, induced 3 days prior to implantation, has activated the inflammatory potential of otherwise immunologically privileged tissue. Subsequent cell implantation was accompanied by reactive astrogliosis, activation of microglia, as well as a massive invasion of macrophages into transplantation sites even if the grafts were placed into contralateral healthy hemispheres, remote from the traumatic lesion. Our results demonstrate a significant post-traumatic inflammatory response, which impairs survival and integration of implanted stem cells and has generally not been taken into account in designs of previous transplantation studies.
authors: Clinic of Neurosurgery, Faculty of Medicine, University of Cologne, Germany.
authors: Marek
authors: M
authors: Molcanyi
authors: Peter
authors: P
authors: Riess
authors: Kristine
authors: K
authors: Bentz
authors: Marc
authors: M
authors: Maegele
authors: Jürgen
authors: J
authors: Hescheler
authors: Bernhard
authors: B
authors: Schäfke
authors: Thorsten
authors: T
authors: Trapp
authors: Edmund
authors: E
authors: Neugebauer
authors: Norfrid
authors: N
authors: Klug
authors: Ute
authors: U
authors: Schäfer
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology; D001930:Brain Injuries / Q000473:pathology* / Q000628:therapy*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016650:Fluorescein-5-isothiocyanate; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007150:Immunohistochemistry; D007249:Inflammation / Q000473:pathology*; D008264:Macrophages / Q000276:immunology / Q000473:pathology; D008297:Male; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000502:physiology
chemical_list: D000941:Antigens; D049452:Green Fluorescent Proteins; D016650:Fluorescein-5-isothiocyanate