Extraction and determination of papaverin in pericarpium papaveris using aqueous two-phase system of poly(ethylene glycol)-(NH4)2SO4 coupled with high-performance liquid chromatography.
Based on aqueous two-phase system (ATPS) of poly(ethylene glycol) (PEG)-(NH4)2SO4, a simple pretreatment approach was developed for the extraction and determination of papaverin in pericarpium papaveris. The influence factors on phase behavior of the ATPS and partition behavior of papaverin was investigated, and partition mechanism based on the hydrophobic interaction between PEG and analyte molecules was proposed. Under the optimal conditions, the extraction efficiencies for papaverin were 93-96%, and the recoveries of the added standard were 97-106% with relative standard deviations of 1.8-2.5%. Combined with a high-performance liquid chromatography (HPLC) method, this extraction technique has been successfully applied to the determination of papaverin in pericarpium papaveris with the detection limit of 2 ng mL(-1) and the linear range of 0.10-10 microg mL(-1). Compared with the conventional liquid-liquid extraction or solid-phase extraction, this method was more environmentally benign, more cost effective and much simpler due to the direct injection of the upper phase into HPLC system.
Cao
Qing
Q
Beijing National Laboratory for Molecular Sciences, The Key Laboratory of Bioorganic Chemistry and Molecular Engineering, Ministry of Education, College of Chemistry, Peking University, Beijing 100871, China.
Li
Shehong
S
He
Chiyang
C
Li
Kean
K
Liu
Feng
F
D000645:Ammonium Sulfate / Q000737:chemistry; D002138:Calibration; D002498:Centrifugation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D010207:Papaver / Q000737:chemistry*; D010208:Papaverine / Q000032:analysis* / Q000302:isolation & purification; D011092:Polyethylene Glycols / Q000737:chemistry
D004365:Drugs, Chinese Herbal; D011092:Polyethylene Glycols; D010208:Papaverine; D000645:Ammonium Sulfate