title: The effect of three-dimensional demineralized bone matrix on in vitro cumulus-free oocyte maturation.
abstract: The physiological role of cumulus cell surrounding oocytes is particularly important for normal cytoplasmic maturation of oocytes. Collagen-based demineralized bone matrix (DBM) is a valuable biomaterial for the three-dimensional (3-D) cell culture. The present study was designed to determine whether in vitro maturation (IVM) of cumulus-free oocytes in mice could be improved by using the 3-D DBM co-culture system. The results indicated that the denuded oocytes cultured in 3-D DBM co-culture system with cumulus cells showed close similarity of cortical granules (CGs) distribution pattern, had more normal maturation-promoting factor (MPF) level and zona pellucida (ZP) hardening level to the in vivo matured oocytes, and the best preimplantation development after being activated by in vitro fertilization (IVF) or parthenogenetic activation. Thus, 3-D DBM collagen scaffold could serve as a tool for fundamental in vitro studies of cells or tissues under the environment that closely assembles the in vivo conditions.
authors: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, No. 3 Nanyitiao, Zhongguancun, Beijing 100080, China.
authors: Suofeng
authors: S
authors: Ma
authors: Hang
authors: H
authors: Lin
authors: Yiliang
authors: Y
authors: Miao
authors: Xinyong
authors: X
authors: Liu
authors: Bin
authors: B
authors: Wang
authors: Jianwu
authors: J
authors: Dai
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D018775:Bone Demineralization Technique / Q000379:methods; D001857:Bone Matrix / Q000737:chemistry* / Q000648:ultrastructure; D002417:Cattle; D002478:Cells, Cultured; D033481:Chromosomes, Mammalian / Q000378:metabolism; D018920:Coculture Techniques / Q000379:methods*; D003094:Collagen / Q000737:chemistry* / Q000378:metabolism; D003594:Cytoplasmic Granules / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005562:Formazans / Q000032:analysis; D016200:Maturation-Promoting Factor / Q000032:analysis; D008540:Meiosis / Q000502:physiology; D000090204:Mesothelin; D051379:Mice; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D010053:Ovary / Q000166:cytology; D010312:Parthenogenesis / Q000187:drug effects / Q000502:physiology; D016062:Porosity; D013324:Strontium / Q000494:pharmacology; D013778:Tetrazolium Salts / Q000032:analysis; D015044:Zona Pellucida / Q000502:physiology
chemical_list: D001672:Biocompatible Materials; D005562:Formazans; C000715528:Msln protein, mouse; D013778:Tetrazolium Salts; C079782:MTT formazan; D003094:Collagen; D016200:Maturation-Promoting Factor; C025700:strontium chloride; D000090204:Mesothelin; D013324:Strontium