title: Proteomics of canine lymphoma identifies potential cancer-specific protein markers.
abstract: OBJECTIVE
  Early diagnosis of cancer is crucial for the success of treatment of the disease, and there is a need for markers whose differential expression between disease and normal tissue could be used as a diagnostic tool. Spontaneously occurring malignancies in pets provide a logical tool for translational research for human oncology. Lymphoma, one of the most common neoplasms in dogs, is similar to human non-Hodgkin's lymphoma and could serve as an experimental model system.
  
  
  METHODS
  Thirteen lymph nodes from normal dogs and 11 lymph nodes from dogs with B-cell lymphoma were subjected to proteomic analysis using two-dimensional PAGE separation and matrix-assisted laser desorption/ionization time-of-flight analysis.
  
  
  RESULTS
  A total of 93 differentially expressed spots was subjected to matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry analysis, and several proteins that showed differential expression were identified. Of these, prolidase (proline dipeptidase), triosephosphate isomerase, and glutathione S-transferase were down-regulated in lymphoma samples, whereas macrophage capping protein was up-regulated in the lymphoma samples.
  
  
  CONCLUSIONS
  These proteins represent potential markers for the diagnosis of lymphoma and should be further investigated in human samples for validation of their utility as diagnostic markers.
authors: Department of Veterinary Medicine and Surgery, University of Missouri, Columbia, Missouri 65211, USA. McCawD@missouri.edu
authors: Dudley L
authors: DL
authors: McCaw
authors: Arvan S
authors: AS
authors: Chan
authors: Andrew L
authors: AL
authors: Stegner
authors: Brian
authors: B
authors: Mooney
authors: Jeffrey N
authors: JN
authors: Bryan
authors: Susan E
authors: SE
authors: Turnquist
authors: Carolyn J
authors: CJ
authors: Henry
authors: Hannah
authors: H
authors: Alexander
authors: Stephen
authors: S
authors: Alexander
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D008198:Lymph Nodes / Q000473:pathology; D008223:Lymphoma / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D009363:Neoplasm Proteins / Q000032:analysis*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins