title: EphrinA1 activates a Src/focal adhesion kinase-mediated motility response leading to rho-dependent actino/myosin contractility.
abstract: Eph receptors and ephrin ligands are widely expressed in epithelial cells and mediate cell repulsive motility through heterotypic cell-cell interactions. Several Ephs, including EphA2, are greatly overexpressed in certain tumors, in correlation with poor prognosis and high vascularity in cancer tissues. The ability of several Eph receptors to regulate cell migration and invasion likely contribute to tumor progression and metastasis. We report here that in prostatic carcinoma cells ephrinA1 elicits a repulsive response that is executed through a Rho-dependent actino/myosin contractility activation, ultimately leading to retraction of the cell body. This appears to occur through assembly of an EphA2-associated complex involving the two kinases Src and focal adhesion kinase (FAK). EphrinA1-mediated repulsion leads to the selective phosphorylation of Tyr-576/577 of FAK, enhancing FAK kinase activity. The repulsive response elicited by ephrinA1 in prostatic carcinoma cells is mainly driven by a Rho-mediated phosphorylation of myosin light chain II, in which Src and FAK activation are required steps. Consequently, Src and FAK are upstream regulators of the overall response induced by ephrinA1/EphA2, instructing cells to retract the cell body and to move away, probably facilitating dissemination and tissue invasion of ephrin-sensitive carcinomas.
authors: Department of Biochemical Sciences, University of Florence, Viale Morgagni 50, 50134 Florence, Italy.
authors: Matteo
authors: M
authors: Parri
authors: Francesca
authors: F
authors: Buricchi
authors: Elisa
authors: E
authors: Giannoni
authors: Giovanna
authors: G
authors: Grimaldi
authors: Tommaso
authors: T
authors: Mello
authors: Giovanni
authors: G
authors: Raugei
authors: Giampietro
authors: G
authors: Ramponi
authors: Paola
authors: P
authors: Chiarugi
mesh_terms: D000199:Actins / Q000378:metabolism*; D024722:Cardiac Myosins / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D036382:Ephrin-A1 / Q000378:metabolism*; D036383:Ephrin-A2 / Q000378:metabolism; D051417:Focal Adhesion Kinase 1 / Q000378:metabolism*; D006801:Humans; D008297:Male; D046912:Multiprotein Complexes / Q000378:metabolism; D018994:Myosin Light Chains / Q000378:metabolism*; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000378:metabolism*; D010766:Phosphorylation; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D011499:Protein Processing, Post-Translational; D036082:Receptor, EphA1 / Q000378:metabolism; D020741:rho GTP-Binding Proteins / Q000378:metabolism*; D019061:src-Family Kinases / Q000378:metabolism*
chemical_list: D000199:Actins; D036382:Ephrin-A1; D036383:Ephrin-A2; D046912:Multiprotein Complexes; D018994:Myosin Light Chains; D009363:Neoplasm Proteins; C107184:myosin light chain 2; D036082:Receptor, EphA1; D051417:Focal Adhesion Kinase 1; D019061:src-Family Kinases; D024722:Cardiac Myosins; D020741:rho GTP-Binding Proteins