Evidence for recent horizontal transfer of gypsy-homologous LTR-retrotransposon gtwin into Drosophila erecta followed by its amplification with multiple aberrations.
Long terminal repeat (LTR) retrotransposon gtwin was initially discovered in silico, and then it was isolated as gypsy-homologous sequence from Drosophila melanogaster strain, G32. The presence of ORF3 suggests, that gtwin, like gypsy, may be an endogenous retrovirus, which can leave the cell and infect another one. Therefore, in this study we decided to investigate the distribution of gtwin in different species of the melanogaster subgroup in order to find out whether gtwin can be transferred horizontally as well as vertically. Gtwin was found in all 9 species of this subgroup, hence it seems to have inhabited the host genomes for a long time. In addition, we have shown that in the Drosophila erecta genome two gtwin families are present. The first one has 93% of identity to D. melanogaster element and is likely to be a descendant of gtwin that existed in Drosophila before the divergence of the melanogaster subgroup species. The other one has >99% of identity to D. melanogaster gtwin. The most reasonable explanation is that this element has been recently horizontally transferred between D. melanogaster and D. erecta. The number and variety of gtwin copies from the "infectious" family suggest that after the horizontal transfer into D. erecta genome, gtwin underwent amplification and aberrations, leading to the rise of its diverse variants.
Kotnova
Alina P
AP
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia. alina_kotnova@mail.ru
Glukhov
Ivan A
IA
Karpova
Nina N
NN
Salenko
Veniamin B
VB
Lyubomirskaya
Natalia V
NV
Ilyin
Yuriy V
YV
D000818:Animals; D002999:Clone Cells; D004247:DNA / Q000302:isolation & purification; D004330:Drosophila / Q000145:classification* / Q000235:genetics*; D005784:Gene Amplification; D022761:Gene Transfer, Horizontal; D049750:Genome, Insect / Q000235:genetics; D008957:Models, Genetic; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D015183:Restriction Mapping; D018626:Retroelements / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D020079:Terminal Repeat Sequences / Q000235:genetics*
D018626:Retroelements; D004247:DNA