title: Effect of HbS in the determination of HbA2 with the Biorad Variant II analyzer.
abstract: OBJECTIVE
  The effect of the presence of HbS in the determination of HbA2 using the Biorad Variant II analyzer.
  
  
  METHODS
  The effect of HbS presence in the samples was quantified using the HELENA SAS-MX alkaline gel electrophoresis kit as the reference method.
  
  
  RESULTS
  The %HbA2 values from the Variant II analyzer and the HELENA SAS-MX alkaline gel electrophoresis kit show a good linear correlation in the absence of HbS. A strong positive bias in the %HbA2 values from the Variant II is apparent in the presence of HbS in the samples, when compared to the alkaline electrophoresis gel.
  
  
  CONCLUSIONS
  The Variant II analyzer gives reliable results for %HbA2 determination when no HbS is detectable in the samples. When HbS is present, the gel electrophoresis method gives more accurate results.
authors: Hematology Laboratory, General Hospital of Poligiros, Poligiros, Greece.
authors: Christoforos
authors: C
authors: Kalleas
authors: Ioannis
authors: I
authors: Tentes
authors: Dimitrios
authors: D
authors: Margaritis
authors: Kostas
authors: K
authors: Anagnostopoulos
authors: Athina
authors: A
authors: Toli
authors: Dimitrios
authors: D
authors: Pendilas
authors: Georgios
authors: G
authors: Bourikas
authors: Constantinos
authors: C
authors: Tsatalas
authors: Alexandros H
authors: AH
authors: Kortsaris
mesh_terms: D001322:Autoanalysis; D002847:Chromatography, Agarose; D002851:Chromatography, High Pressure Liquid; D005189:False Positive Reactions; D006443:Hemoglobin A2 / Q000032:analysis* / Q000302:isolation & purification; D006451:Hemoglobin, Sickle / Q000494:pharmacology*; D006801:Humans
chemical_list: D006451:Hemoglobin, Sickle; D006443:Hemoglobin A2