title: Interaction of the protein C activation peptide with platelets.
abstract: The peptide NH(2)-DTEDQEDQVDPR-COOH is released during activation of protein C zymogen. We measured the effect of a synthetic peptide with an amino acid sequence similar to that of the natural peptide on platelets from healthy individuals using platelet aggregometry. We found that this synthetic peptide inhibits platelet aggregation induced by thrombin; furthermore, it diminishes mobilization of intraplatelet calcium. Molecular docking showed weak interaction between the synthetic peptide and thrombin. Our findings suggest that this synthetic peptide may interact with a receptor located on the platelet cell membrane.
authors: Centro de Investigación en Ciencias Médicas y Biológicas, Facultad de Medicina y Cirugía, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca, México.
authors: Ruth
authors: R
authors: Martínez-Cruz
authors: María Del S Pina
authors: Mdel S
authors: Canseco
authors: Jael
authors: J
authors: Lopez-Martínez
authors: Pedro A Hernández
authors: PA
authors: Cruz
authors: Eduardo
authors: E
authors: Pérez-Campos
authors: Margarito Martínez
authors: MM
authors: Cruz
authors: Félix Córdoba
authors: FC
authors: Alva
authors: Abraham
authors: A
authors: Majluf-Cruz
authors: Edgar
authors: E
authors: Zenteno
authors: Alejandro
authors: A
authors: Ruiz-Argüelles
mesh_terms: D000595:Amino Acid Sequence; D001792:Blood Platelets / Q000737:chemistry / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D004792:Enzyme Precursors / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism*; D015539:Platelet Activation / Q000187:drug effects*; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011956:Receptors, Cell Surface / Q000378:metabolism; D018179:Receptors, Thrombin / Q000378:metabolism; D013917:Thrombin / Q000494:pharmacology*
chemical_list: D004792:Enzyme Precursors; D009842:Oligopeptides; D010975:Platelet Aggregation Inhibitors; D011956:Receptors, Cell Surface; D018179:Receptors, Thrombin; C047135:protein C activator peptide; D013917:Thrombin; D002118:Calcium