title: Inhibition of photophosphorylation and electron transport chain in thylakoids by lasiodiplodin, a natural product from Botryosphaeria rhodina.
abstract: Four natural products were isolated from the fungus Botryosphaeria rhodina, and their effects on photosynthesis were tested. Only lasiodiplodin (1) inhibited ATP synthesis and electron flow from water to methylviologen; therefore, it acts as a Hill reaction inhibitor in freshly lysed spinach thylakoids. Photosystem I and II and partial reactions as well as ATPase were measured in the presence of 1. Three new different sites of 1 interaction and inhibition were found: one at CF1, the second in the water-splitting enzyme, and the third at the electron-transfer path between P680 and QA; these targets are different from that of the synthetic herbicides present. Electron transport chain inhibition by 1 was corroborated by fluorescence induction kinetics studies.
authors: Departamento de Bioquímica, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510 México DF, Mexico.
authors: Thiago A M
authors: TA
authors: Veiga
authors: Sebastião C
authors: SC
authors: Silva
authors: Archundia-Camacho
authors: AC
authors: Francisco
authors: Edson R
authors: ER
authors: Filho
authors: Paulo C
authors: PC
authors: Vieira
authors: João B
authors: JB
authors: Fernandes
authors: Maria F G F
authors: MF
authors: Silva
authors: Manfred W
authors: MW
authors: Müller
authors: Blas
authors: B
authors: Lotina-Hennsen
mesh_terms: D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors; D000255:Adenosine Triphosphate / Q000096:biosynthesis; D001203:Ascomycota / Q000737:chemistry*; D004579:Electron Transport / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D010785:Photophosphorylation / Q000187:drug effects*; D018515:Plant Leaves / Q000648:ultrastructure; D018724:Spinacia oleracea / Q000648:ultrastructure; D020524:Thylakoids / Q000187:drug effects* / Q000378:metabolism*; D015025:Zearalenone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; C417552:lasiodiplodin; D015025:Zearalenone; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases