Simultaneous determination of residual stilbenes and stilbene metabolites in animal tissue by liquid chromatography-tandem mass spectrometry.
A liquid chromatographic (LC)-tandem mass spectrometry (MS/MS) method was developed for simultaneous determination of stilbenes, diethylstilbestrol (DES), hexestrol (HEX), and dienoestrol (DEN) in animal tissue. Sample clean-up and analyte enrichment was performed by automated solid-phase extraction (ASPE) with a silica gel cartridge. Detection capabilities (CCbeta) related to the transition products of lowest abundance for the method were 0.04-0.45 ng g(-1) in tissue and were achieved using atmospheric pressure chemical ionization (APCI) in negative mode. The use of an internal standard in combination with the simplified sample preparation led to a sensitive and reliable analytical method. The recovery level of the method was 84-108% for DES and DEN between 0.5 and 5 ng g(-1), and 59-87% for HEX between 0.25 and 2.5 ng g(-1).
Hong
Xu
X
Tianjin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, 300201 Tianjin, China. hongxxu@yahoo.com.cn
Long
Gu
G
Jia
He
H
Anqing
Lin
L
Danzhou
Tang
T
D000818:Animals; D001274:Atmospheric Pressure; D002853:Chromatography, Liquid / Q000379:methods*; D012015:Reference Standards; D012680:Sensitivity and Specificity; D013267:Stilbenes / Q000032:analysis* / Q000378:metabolism; D053719:Tandem Mass Spectrometry / Q000379:methods*
D013267:Stilbenes