title: Biomolecular surface coating to enhance orthopaedic tissue healing and integration.
abstract: Implant osseointegration is a prerequisite for clinical success in orthopaedic and dental applications, many of which are restricted by loosening. Biomaterial surface modification approaches, including calcium-phosphate ceramic coatings and macro/microporosity, have had limited success in promoting integration. To improve osseointegration, titanium surfaces were coated with the glycine-phenylalanine-hydroxyproline-glycine-glutamate-arginine (GFOGER) collagen-mimetic peptide, selectively promoting alpha2beta1 integrin binding, a crucial event for osteoblastic differentiation. Titanium surfaces presenting GFOGER triggered osteoblastic differentiation and mineral deposition in bone marrow stromal cells, leading to enhanced osteoblastic function compared to unmodified titanium. Furthermore, this integrin-targeted coating significantly improved in vivo peri-implant bone regeneration and osseointegration, as characterized by bone-implant contact and mechanical fixation, compared to untreated titanium in a rat cortical bone-implant model. GFOGER-modified implants also significantly enhanced osseointegration compared to surfaces modified with full-length type I collagen, highlighting the importance of presenting specific biofunctional domains within the native ligand. In addition, this biomimetic implant coating is generated using a simple, single-step procedure that readily translates to a clinical environment with minimal processing and cytotoxicity concerns. Therefore, this study establishes a biologically active and clinically relevant implant-coating strategy that enhances bone repair and orthopaedic implant integration.
authors: Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
authors: Catherine D
authors: CD
authors: Reyes
authors: Timothy A
authors: TA
authors: Petrie
authors: Kellie L
authors: KL
authors: Burns
authors: Zvi
authors: Z
authors: Schwartz
authors: Andrés J
authors: AJ
authors: García
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry; D001854:Bone Marrow Cells / Q000166:cytology; D001861:Bone Regeneration / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D024042:Collagen Type I / Q000378:metabolism; D005269:Femur / Q000166:cytology; D015870:Gene Expression; D038982:Integrin alpha2beta1 / Q000378:metabolism; D008297:Male; D008422:Materials Testing; D009842:Oligopeptides / Q000737:chemistry; D016348:Osseointegration / Q000502:physiology*; D010006:Osteoblasts / Q000166:cytology / Q000201:enzymology / Q000378:metabolism / Q000502:physiology; D019736:Prostheses and Implants; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology; D013499:Surface Properties; D013977:Tibia / Q000166:cytology; D014025:Titanium / Q000737:chemistry
chemical_list: D020099:Coated Materials, Biocompatible; D024042:Collagen Type I; D038982:Integrin alpha2beta1; D009842:Oligopeptides; D014025:Titanium; D000469:Alkaline Phosphatase; D002118:Calcium