title: Modification of host erythrocyte membranes by trypsin and chymotrypsin treatments and effects on the in vitro growth of bovine and equine Babesia parasites.
abstract: In the present study, we investigated the effects of protease pretreatments of host erythrocytes (RBC) on the in vitro growth of bovine Babesia parasites (Babesia bovis and B. bigemina) and equine Babesia parasites (B. equi and B. caballi). The selected proteases, trypsin and chymotrypsin, clearly modified several membrane proteins of both bovine and equine RBC, as demonstrated by SDS-PAGE analysis; however, the protease treatments also modified the sialic acid content exclusively in bovine RBC, as demonstrated by lectin blot analysis. An in vitro growth assay using the protease-treated RBC showed that the trypsin-treated bovine RBC, but not the chymotrypsin-treated ones, significantly reduced the growth of B. bovis and B. bigemina as compared to the control. In contrast, the growth of B. equi and B. caballi was not affected by any of these proteases. Thus, the bovine, but not the equine, Babesia parasites require the trypsin-sensitive membrane (sialoglyco) proteins to infect the RBC.
authors: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
authors: Masashi
authors: M
authors: Okamura
authors: Naoaki
authors: N
authors: Yokoyama
authors: Noriyuki
authors: N
authors: Takabatake
authors: Kazuhiro
authors: K
authors: Okubo
authors: Yuzuru
authors: Y
authors: Ikehara
authors: Ikuo
authors: I
authors: Igarashi
mesh_terms: D000818:Animals; D001403:Babesia / Q000145:classification / Q000254:growth & development*; D001404:Babesiosis / Q000469:parasitology / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology; D002478:Cells, Cultured; D002918:Chymotrypsin / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects / Q000469:parasitology*; D006734:Horse Diseases / Q000469:parasitology; D006736:Horses; D008565:Membrane Proteins / Q000378:metabolism; D014357:Trypsin / Q000494:pharmacology*
chemical_list: D008565:Membrane Proteins; D002918:Chymotrypsin; D014357:Trypsin