title: Crossing the barrier: evolution and spread of a major class of mosaic pbp2x in Streptococcus pneumoniae, S. mitis and S. oralis.
abstract: A paradigm for Streptococcus interspecies gene transfer is represented by the mosaic pbp genes encoding the target enzymes for beta-lactam antibiotics, the penicillin-binding proteins, in Streptococcus pneumoniae. We investigated a collection of oral streptococci from three continents by comprehensive multi-locus sequence typing analysis in order to trace the origin of a mosaic block belonging to a dominant family of mosaic pbp2x implicated in penicillin resistance of S. pneumoniae. One widespread family of mosaic pbp2x occurred in all three distinct clusters of S. pneumoniae, Streptococcus mitis and Streptococcus oralis, documenting independent inter- and intraspecies recombination events. Moreover, potential ancestor genes of this mosaic block could be identified in two penicillin-susceptible S. mitis strains from South Africa and Spain, facilitating the identification of pbp2x mutations relevant for resistance development.
authors: Department of Microbiology, University of Kaiserslautern, Paul Ehrlich Str. 23, D-67663 Kaiserslautern, Germany.
authors: Fang
authors: F
authors: Chi
authors: Oliver
authors: O
authors: Nolte
authors: Carina
authors: C
authors: Bergmann
authors: Margaret
authors: M
authors: Ip
authors: Regine
authors: R
authors: Hakenbeck
mesh_terms: D003434:Crossing Over, Genetic / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis; D010403:Penicillin Resistance / Q000235:genetics; D046915:Penicillin-Binding Proteins / Q000235:genetics*; D010406:Penicillins / Q000494:pharmacology; D034361:Streptococcus mitis / Q000187:drug effects / Q000235:genetics*; D019533:Streptococcus oralis / Q000187:drug effects / Q000235:genetics*; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D046915:Penicillin-Binding Proteins; D010406:Penicillins; C072223:PBP 2x protein, Streptococcus