Combinatorial mutasynthesis of flavonoid analogues from acrylic acids in microorganisms.
Flavonoids are plant secondary metabolites often used as nutraceutical supplements, but a growing number of unnatural flavonoids are being investigated as therapeutic agents. Cultures of Saccharomyces cerevisiae expressing recombinant flavonoid enzymes, including 4-coumaroyl:CoA ligase (4CL), chalcone synthase (CHS), chalcone isomerase (CHI), and flavanone 3beta-hydroxylase (FHT), produced novel flavanones and dihydroflavonols when fed with a number of aromatic acrylic acids. The flavonoid network also exhibited broad substrate specificity by converting muconic acid into a unique polypropanoid.
Chemler
Joseph A
JA
State University of New York at Buffalo, Department of Chemical and Biological Engineering, 303 Furnas Hall, Buffalo, NY 14260, USA.
Yan
Yajun
Y
Leonard
Effendi
E
Koffas
Mattheos A G
MA
D000179:Acrylates / Q000737:chemistry* / Q000378:metabolism*; D005419:Flavonoids / Q000096:biosynthesis* / Q000737:chemistry*; D006859:Hydrogen / Q000737:chemistry; D015394:Molecular Structure; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D000179:Acrylates; D005419:Flavonoids; D006859:Hydrogen; C036658:acrylic acid