[Expression of integrin genes in heart of septic rat].
OBJECTIVE
To investigate the expression profile of integrin genes in heart of septic rat and relevant mechanisms responsible for sepsis-induced heart injury.


METHODS
Twelve 3-month-old male Wistar rats were randomized to 2 equal groups, sepsis model group (CLP group) undergoing ligation and perforation with needle of the distal caecum so as to establish sepsis model, and sham operation group (Sham group), undergoing sham operation only serve as controls. Twenty-four later the hearts of rats were rapidly excised. After determination of the hemodynamic parameters by using Langendorff apparatus, the isolated hearts were cut into 2 parts vertically to undergo histopathological examination and analysis of the expression of integrin genes by oligonucleotide microarrays respectively.


RESULTS
No overt pathological changes were detected in the hearts of septic rats, however, the cardiac output, stroke volume, heart rate, left ventricular developed pressure, left ventricular end-diastolic pressure, and maximum rate of left ventricular pressure rise of the CLP group were 29.4+/-3.3 ml/min, 0.12+/-0.01 ml, 256+/-6 bpm, 75.6+/-4.9 mm Hg, 7.5+/-0.3 mm Hg, and 2167+/-159 mm Hg/s respectively, all significantly than those of the Sham group (57.8+/-2.4 ml/min, 0.18+/-0.01 ml, 302+/-12 bpm, 90.0+/-2.7 mmHg, 8.0+/-0.3 mmHg. and 2601+/-34 mmHg/s respectively, all P<0.01). Microarray analysis showed that 20 out of 24 integrin genes were up-regulated by more than 2 times in the septic rat heart, among which the integrin alphaV and beta2 genes were upregulated and the expression of integrin beta1 gene was relatively insufficient.


CONCLUSIONS
Maladjustment in expression of integrin genes is present in the septic rat heart. Up-regulation of integrin alphaV and beta2 genes may accelerate the heart injury mediated by inflammatory mediators, and the relatively insufficient expression of integrin beta1 gene may contribute to cardiac dysfunction.
Zhang
Zhao-cai
ZC
Intensive Care Unit, Zhejiang Hospital, Hangzhou 310013, China.
Yan
Jing
J
Cai
Guo-long
GL
Yu
Yi-hua
YH
Hu
Cai-bao
CB
D000818:Animals; D018821:CD18 Antigens / Q000235:genetics; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D006321:Heart / Q000503:physiopathology; D039564:Integrin alphaV / Q000235:genetics; D016023:Integrins / Q000235:genetics*; D008297:Male; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology
D018821:CD18 Antigens; D039564:Integrin alphaV; D016023:Integrins