Two-dimensional capillary electrophoresis using tangentially connected capillaries.
A novel type of fused silica capillary system is described where channels with circular cross-sections are tangentially in contact with each other and connected through a small opening at the contact area. Since the channels are not crossing each other in the same plane, the capillaries can easily be filled with different solutions, i.e. different solutions will be in contact with each other at the contact point. The system has been used to perform different types of two-dimensional separations and the complete system is fully automated where a high voltage switch is used to control the location of the high voltage in the system. Using two model compounds it is demonstrated that a type of two-dimensional separation can be performed using capillary zone electrophoresis at two different pH values. It is also shown that a compound with acid/base properties can be concentrated using a dynamic pH junction mechanism when transferred from the first separation to the second separation. In addition, the system has been used to perform a comprehensive two-dimensional capillary electrophoresis separation of tryptic digest of bovine serum albumin using capillary zone electrophoresis followed by micellar electrokinetic chromatography.
Sahlin
Eskil
E
Department of Chemistry, Göteborg University, 412 96 Göteborg, Sweden. eskil.sahlin@sp.se
D002723:Chlorobenzoates / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods*; D004867:Equipment Design; D006588:Hexanones / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D011492:Protein Hydrolysates / Q000302:isolation & purification; D012710:Serum Albumin, Bovine / Q000737:chemistry; D014357:Trypsin / Q000378:metabolism
D002723:Chlorobenzoates; D006588:Hexanones; D011492:Protein Hydrolysates; D012710:Serum Albumin, Bovine; C008374:mesityl oxide; C060415:2-chlorobenzoic acid; D014357:Trypsin