title: Label-free detection of Babesia bovis infected red blood cells using impedance spectroscopy on a microfabricated flow cytometer.
abstract: Impedance spectroscopy is a powerful tool for label-free analysis and characterisation of living cells. In this work, we achieved the detection of Babesia bovis infected red blood cells using impedance spectroscopy on a microfabricated flow cytometer. The cellular modifications caused by the intracellular parasite result in a shift in impedance which can be measured dielectrically. Thus, a rapid cell-by-cell detection with microliter amounts of reagents is possible. Unlike other diagnostic tests, this method does not depend on extensive sample pre-treatment or expensive chemicals and equipment.
authors: Laboratory of Microsystems, Ecole Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland. kuettel@nano.mavt.ethz.ch
authors: Claudia
authors: C
authors: Küttel
authors: Elisabete
authors: E
authors: Nascimento
authors: Nicolas
authors: N
authors: Demierre
authors: Tiago
authors: T
authors: Silva
authors: Thomas
authors: T
authors: Braschler
authors: Philippe
authors: P
authors: Renaud
authors: Abel G
authors: AG
authors: Oliva
mesh_terms: D000818:Animals; D016793:Babesia bovis / Q000302:isolation & purification*; D001404:Babesiosis / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000469:parasitology; D017097:Electric Impedance; D004912:Erythrocytes / Q000469:parasitology*; D005434:Flow Cytometry / Q000295:instrumentation*; D010274:Parasitology / Q000379:methods; D013057:Spectrum Analysis / Q000379:methods*