[Effects of methyl jasmonat on accumulation and release of tanshinones in suspension cultures of Salvia miltiorrhiza hairy root].
OBJECTIVE
To study the effects of methyl jasmonate (MJ) on the accumulation and release of tanshinones in suspension cultures of Salvia miltiorrhiza hairy roots.


METHODS
After 18 day's suspension culture of S. miltiorrhiza hairy roots induced by Agrobacterium rhizogenes ATCC15834, the chemical elicitor--methyl jasmonat was added into 6-7V suspension cultures and at the same time, tanshinones contents (including cryptotanshinone and tanshinone II(A)) on the day 2, 6 and 9, after dealing with MJ, was quantified by HPLC.


RESULTS
After dealing with MJ on the day 2, 6 and 9, the concentration of cryptotanshinone reached to 0.039, 0.204, 0.571 mg x g(-1) respectively,and tanshinone II(A) reached 0.251, 0.601 and 1.563 mg x g(-1) respectively. After 9 day's treatment by MJ, the maximum increase of cryptotanshinone and tanshinone II(A) were 23.8 fold and 6.2 fold higher than that of the control respectively.


CONCLUSIONS
MJ could stimulate the accumulation of tanshinones in S. miltiorrhiza and have released them into the culture medium.
Wang
Xue-Yong
XY
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Cui
Guang-Hong
GH
Huang
Lu-Qi
LQ
Qiu
De-You
DY
D045784:Abietanes; D000085:Acetates / Q000494:pharmacology*; D046508:Culture Techniques; D003517:Cyclopentanes / Q000494:pharmacology*; D054883:Oxylipins / Q000494:pharmacology*; D010616:Phenanthrenes / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D010946:Plants, Medicinal / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D027561:Salvia miltiorrhiza / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism
D045784:Abietanes; D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010616:Phenanthrenes; D010937:Plant Growth Regulators; C021751:tanshinone; C037886:cryptotanshinone; C072239:methyl jasmonate