Reference materials for the evaluation of pre-mortem variant Creutzfeldt-Jakob disease diagnostic assays.
OBJECTIVE
A standard panel of materials is needed for the evaluation of assays being developed for the diagnosis of variant Creutzfeldt-Jakob disease.


METHODS
Tissues from human and animals incubating transmissible spongiform encephalopathy disease have been prepared, aliquoted and where possible characterized by in vitro methods.


RESULTS
A standardized preparation of materials has been generated.


CONCLUSIONS
Large-scale preparations of tissues and blood fractions can be used to directly compare the sensitivities of assays using different formats.
Cooper
J K
JK
CJD Resource Centre, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar EN6 3QG, UK. jcooper@nibsc.ac.uk
Ladhani
K
K
Minor
P D
PD
D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western / Q000379:methods / Q000592:standards; D001923:Brain Chemistry; D007562:Creutzfeldt-Jakob Syndrome / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D004247:DNA / Q000235:genetics; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods / Q000592:standards; D018620:PrPSc Proteins / Q000097:blood / Q000235:genetics / Q000302:isolation & purification / Q000592:standards; D011328:Prions / Q000097:blood / Q000235:genetics / Q000302:isolation & purification* / Q000592:standards; D011487:Protein Conformation; D012015:Reference Standards; D012608:Scrapie / Q000097:blood / Q000175:diagnosis / Q000235:genetics; D012756:Sheep; D013154:Spleen / Q000737:chemistry; D014018:Tissue Distribution
D018620:PrPSc Proteins; D011328:Prions; D004247:DNA