Imaging the impact of cortical microcirculation on synaptic structure and sensory-evoked hemodynamic responses in vivo.
In vivo two-photon microscopy was used to image in real time dendrites and their spines in a mouse photothrombotic stroke model that reduced somatosensory cortex blood flow in discrete regions of cortical functional maps. This approach allowed us to define relationships between blood flow, cortical structure, and function on scales not previously achieved with macroscopic imaging techniques. Acute ischemic damage to dendrites was triggered within 30 min when blood flow over >0.2 mm(2) of cortical surface was blocked. Rapid damage was not attributed to a subset of clotted or even leaking vessels (extravasation) alone. Assessment of stroke borders revealed a remarkably sharp transition between intact and damaged synaptic circuitry that occurred over tens of mum and was defined by a transition between flowing and blocked vessels. Although dendritic spines were normally ~13 microm from small flowing vessels, we show that intact dendritic structure can be maintained (in areas without flowing vessels) by blood flow from vessels that are on average 80 microm away. Functional imaging of intrinsic optical signals associated with activity-evoked hemodynamic responses in somatosensory cortex indicated that sensory-induced changes in signal were blocked in areas with damaged dendrites, but were present ~400 microm away from the border of dendritic damage. These results define the range of influence that blood flow can have on local cortical fine structure and function, as well as to demonstrate that peri-infarct tissues can be functional within the first few hours after stroke and well positioned to aid in poststroke recovery.
Zhang
Shengxiang
S
Kinsmen Laboratory of Neurological Research, Department of Psychiatry, University of British Columbia, Vancouver, British Columbia, Canada.
Murphy
Timothy H
TH
D000818:Animals; D002545:Brain Ischemia / Q000473:pathology; D003712:Dendrites / Q000473:pathology; D005552:Forelimb; D006614:Hindlimb; D051379:Mice; D008810:Mice, Inbred C57BL; D008833:Microcirculation / Q000502:physiology*; D008853:Microscopy; D012395:Rose Bengal; D013003:Somatosensory Cortex / Q000098:blood supply* / Q000648:ultrastructure; D020521:Stroke / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology; D013569:Synapses / Q000648:ultrastructure*
D012395:Rose Bengal