title: Sensitive determination of ultra-trace nitric oxide in blood using derivatization-polymer monolith microextraction coupled with reversed-phase high-performance liquid chromatography.
abstract: Nitric oxide (NO) plays a very important role in human blood system. In this work, a novel approach has been developed for the quantitation of ultra-trace NO derivatized with 1,3,5,7-tetramethyl-8-(3',4'-diaminophenyl)-difluoroboradiaza-s-indacene (DAMBO) using a polymer monolith microextraction (PMME) with a poly(methacrylic acid-ethylene glycol dimethacrylate) (MAA-EGDMA) monolith in conjunction with high-performance liquid chromatography (HPLC). Both derivatization and PMME conditions have been optimized in detail. The detection limit of derivatized NO was 2x10(-12) mol L(-1) (signal to noise=3) and linear range was 9x10(-11)-4.5x10(-8) mol L(-1). The proposed DAMBO-based derivatization-PMME-HPLC-fluorescence detection method has been successfully applied for the determination of NO in 10 microL blood samples of healthy persons and patients suffering from ischemic cardio-cerebrovascular diseases with recoveries varying from 87.40 to 91.60%.
authors: Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
authors: Ke-Jing
authors: KJ
authors: Huang
authors: Min
authors: M
authors: Zhang
authors: Wan-Zhen
authors: WZ
authors: Xie
authors: Hua-Shan
authors: HS
authors: Zhang
authors: Yu-Qi
authors: YQ
authors: Feng
authors: Hong
authors: H
authors: Wang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003920:Diabetes Mellitus / Q000097:blood; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood / Q000150:complications; D006973:Hypertension / Q000097:blood / Q000150:complications; D008832:Microchemistry; D009569:Nitric Oxide / Q000097:blood*; D011109:Polymethacrylic Acids; D012680:Sensitivity and Specificity; D052617:Solid Phase Microextraction; D020521:Stroke / Q000097:blood
chemical_list: D011109:Polymethacrylic Acids; C497090:poly((ethylene glycol dimethacrylate)-co-(methacrylic acid)); D009569:Nitric Oxide