Evaluation of Daphne genkwa diterpenes: fingerprint and quantitative analysis by high performance liquid chromatography.
Daphne genkwa contains a novel class of anticancer diterpene esters that inhibit DNA topoisomerase I. Fingerprint and quantitative analysis by HPLC were performed in order to characterise and evaluate D. genkwa. A standard fingerprint of Daphne diterpene esters from the root extract was first established by HPLC-UV, and the major peaks in the fingerprint profile were preliminarily determined using HPLC-MS. The principal Daphne diterpene esters, yuanhuacine (1), yuanhuadine (2), yuanhuajine (3) and yuanhuagine (4), were isolated and identified using a combination of UV, IR, MS, 1H-NMR and 13C-NMR spectral data. Quantitative analysis indicated that 1 was the principal component in the root, and that 2 was the major component in the buds. The average extraction rates of 1 and 2 were 0.0151 and 0.0033% (n=10) from the root, respectively, and 0.0020 and 0.0078% (n=3) from the buds, respectively.
Zhang
Shixuan
S
State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116012, People's Republic of China. zhangsx56@yahoo.com.cn
Zhang
Fenghong
F
Li
Xiaona
X
Dong
Weiqun
W
Wen
Luohan
L
Wang
Shisheng
S
D000465:Algorithms; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D032364:Daphne / Q000737:chemistry*; D004224:Diterpenes / Q000032:analysis* / Q000737:chemistry; D004952:Esters / Q000032:analysis; D013058:Mass Spectrometry; D018517:Plant Roots / Q000737:chemistry; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
D004224:Diterpenes; D004952:Esters