title: Experimental prediction of the wavelength-dependent path-length factor for optical intrinsic signal analysis.
abstract: Analysis of the optical intrinsic signal of an exposed cortex has been applied to measurement of functional brain activation. It is important for accurate measurement of concentration changes in oxygenated hemoglobin and deoxygenated hemoglobin to consider the wavelength dependence of the mean optical path lengths for the reflectance of cortical tissue. A method is proposed to experimentally estimate the wavelength dependence of the mean optical path length in cortical tissue from the multispectral reflectance of the exposed cortex without any additional instruments. The trend in the wavelength dependence of the mean optical path length estimated by the proposed method agrees with that estimated by the model-based prediction, whereas the magnitude of the wavelength dependence predicted by the proposed method is greater than that of the model-based prediction. The experimentally predicted mean optical path length minimizes the difference in the measured changes in the concentrations of the oxygenated hemoglobin and deoxygenated hemoglobin calculated from different wavelength pairs.
authors: Department of Electronics and Electrical Engineering, Keio University, Yokohama, Japan. koichiro@okd.elec.keio.ac.jp
authors: Koichiro
authors: K
authors: Sakaguchi
authors: Tomoya
authors: T
authors: Tachibana
authors: Shunsuke
authors: S
authors: Furukawa
authors: Takushige
authors: T
authors: Katsura
authors: Kyoko
authors: K
authors: Yamazaki
authors: Hideo
authors: H
authors: Kawaguchi
authors: Atsushi
authors: A
authors: Maki
authors: Eiji
authors: E
authors: Okada
mesh_terms: D000818:Animals; D001303:Auditory Cortex / Q000502:physiology*; D001931:Brain Mapping / Q000379:methods*; D003198:Computer Simulation; D005072:Evoked Potentials, Auditory / Q000502:physiology*; D006168:Guinea Pigs; D006454:Hemoglobins / Q000032:analysis*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008027:Light; D008297:Male; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D008959:Models, Neurological; D012542:Scattering, Radiation
chemical_list: D006454:Hemoglobins