Fast determination of conditions for maximum dynamic capacity in cation-exchange chromatography of human monoclonal antibodies.
Dynamic binding capacity (DBC) measurements of cation-exchange resins were performed with two human monoclonal antibodies. DBC showed a pH dependent maximum, which was shifted to lower pH values with increasing buffer concentrations and increasing salting-out effect of the buffer anion according to the Hofmeister series. As this downshift correlates well with zeta potential values, a measurement of the latter allows the determination of the pH value for maximum DBC under a given set of conditions. Thus, the use of zeta potential values can accelerate the purification process development and helps to understand the protein adsorption mechanism.
Faude
Alexander
A
Institute of Cell Biology and Immunology, University of Stuttgart, Stuttgart, Germany. alexander.faude@izi.uni-stuttgart.de
Zacher
Dörthe
D
Müller
Egbert
E
Böttinger
Heiner
H
D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D002411:Cation Exchange Resins; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration
D000911:Antibodies, Monoclonal; D002411:Cation Exchange Resins