title: Expression of alphaV and beta3 integrin subunits during implantation in pig.
abstract: Integrin are adhesion molecules involved in uterine-conceptus interactions during the perimplantation period. In this study, the expression of alphaV and beta3 integrin subunits in endometrium during implantation in pigs was investigated. The immunohistochemical location was performed on paraformaldehyde-fixed, paraffin-embedded tissue sections, and the mRNA expression of alphaV was detected in endometrium. In addition, serum levels of estradiol, progesterone, follicle-stimulating hormone, and luteinizing hormone were measured on Days 0, 12, 18, and 25 of pregnancy. The results indicate that endometrium expressed integrin alphaV and beta3 in all stages examined. The most intensive staining for integrin alphaV and beta3 was observed in endometrial stroma in porcine pregnancy on Day 18. The mRNA of alphaV integrin strongly expressed on Day 18, and moderately expressed on Days 12 and 25. The correlation between serum hormone level and the mRNA expression of alphaV integrin was not significant. The expression patterns of integrin alphaV and beta3 during implantation provide insights into the important physiological function of alphaVbeta3 integrin in pig, and the strong expression of integrin alphaV and beta3 in mid-implantation may indicate its crucial role in successful implantation and embryo survival.
authors: College of Animal Science and Technology, China Agricultural University, Beijing, People's Republic of China.
authors: Haichao
authors: H
authors: Lin
authors: Xin
authors: X
authors: Wang
authors: Guifen
authors: G
authors: Liu
authors: Jinlian
authors: J
authors: Fu
authors: Aiguo
authors: A
authors: Wang
mesh_terms: D000818:Animals; D010064:Embryo Implantation; D004717:Endometrium / Q000737:chemistry / Q000378:metabolism; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D007150:Immunohistochemistry; D039564:Integrin alphaV / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D039661:Integrin beta3 / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000097:blood; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D034421:Sus scrofa / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D039564:Integrin alphaV; D039661:Integrin beta3; D012333:RNA, Messenger; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone