title: Susceptibility of type 2 diabetic mice to low-virulence bacterial infection: induction of abscess formation by gingipain-deficient Porphyromonas gingivalis.
abstract: OBJECTIVE
  Type 2 diabetes mellitus is considered an important risk factor of adult periodontitis. However, recent studies have revealed that the subgingival microbial flora of diabetes mellitus patients does not differ from that of healthy individuals. In this study, we examined the response of type 2 diabetes mellitus hosts to low-virulence bacteria in a murine abscess model.
  
  
  METHODS
  Porphyromonas gingivalis ATCC 33277 or KDP128 (rgpA rgpB kgp) were injected into two mouse strains - C57BL/6J and its derivative, KK/A(Y), which becomes diabetic spontaneously.
  
  
  RESULTS
  Lesions of KK/A(Y) mice injected with either low-virulence P. gingivalis KDP128 or wild-type 33277 were significantly larger than those of C57BL/6J mice injected with the same strains. Histologically, more neutrophils and macrophages migrated to the lesions in the KK/A(Y) mice injected with P. gingivalis 33277 and KDP128 compared with those of C57BL/6J mice injected with the same respective strains.
  
  
  CONCLUSIONS
  These results suggest that severe inflammation is observed in response to low-virulence bacteria in addition to the highly virulent bacteria in type 2 diabetes mellitus hosts.
authors: Section of Periodontology, Department of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka, Japan.
authors: Y
authors: Y
authors: Ishihara
authors: H
authors: H
authors: Anan
authors: M
authors: M
authors: Yoneda
authors: K
authors: K
authors: Maeda
authors: T
authors: T
authors: Hirofuji
mesh_terms: D000038:Abscess / Q000382:microbiology*; D018829:Adhesins, Bacterial; D000818:Animals; D003546:Cysteine Endopeptidases / Q000172:deficiency; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000382:microbiology*; D004198:Disease Susceptibility / Q000382:microbiology; D005260:Female; D000080867:Gingipain Cysteine Endopeptidases; D008264:Macrophages; D051379:Mice; D008807:Mice, Inbred BALB C; D016966:Porphyromonas gingivalis / Q000472:pathogenicity*; D014774:Virulence
chemical_list: D018829:Adhesins, Bacterial; D000080867:Gingipain Cysteine Endopeptidases; D003546:Cysteine Endopeptidases