Karyotype, centric fusion polymorphism and chromosomal aberrations in captive-born mountain reedbuck (Redunca fulvorufula).
Chromosomes of fourteen captive-born mountain reedbucks (Redunca fulvorufula) have been investigated. The diploid chromosome number was 2n = 56 (FN = 60). The mountain reedbuck karyotype consists of 26 acrocentric and two biarmed chromosome pairs resulting from two centric fusions involving chromosomes 2 and 25, and 6 and 10, respectively. In some animals, 57 chromosomes were detected. Variation in the diploid number was found to be due to polymorphism for the centric fusion 6;10. Both X and Y chromosomes are large and acrocentric. The entire Y chromosome and the proximal part of the X chromosome consist of heterochromatin. The chromosomes X, 9 and 14 appeared to be of caprine type. Chromosome aberrations have been detected in two of the 14 animals investigated. A de novo formed Robertsonian translocation rob(6;13) was found in one female heterozygous for the fusion 6;10. CBG-banding revealed one block of centromeric heterochromatin in the de novo formed translocation rob(6;13) and also in the evolutionarily fixed centric fusions 6;10 and 2;25. One examined male homozygous for fusion 6;10, had a mosaic 56,XY/57,XYY karyotype, with 11% of analyzed cells containing two Y chromosomes. The findings were confirmed by cross-species fluorescence in situ hybridization (FISH) with bovine (Bos taurus L.) chromosome painting probes. The study demonstrates the relevance of cytogenetic screening in captive animals from zoological gardens.
Rubes
J
J
Veterinary Research Institute, Brno, Czech Republic. rubes@vri.cz
Pagacova
E
E
Kopecna
O
O
Kubickova
S
S
Cernohorska
H
H
Vahala
J
J
Di Berardino
D
D
D000818:Animals; D002503:Centromere / Q000235:genetics*; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D005260:Female; D006579:Heterozygote; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D008677:Metaphase; D010375:Pedigree; D011110:Polymorphism, Genetic; D012418:Ruminants / Q000235:genetics*; D014960:X Chromosome / Q000235:genetics / Q000378:metabolism