Effect of a soy protein-based diet on ribonucleic acid metabolism in the small intestinal mucosa of goat kids.
This study was designed to investigate the effect of soy protein inclusion in milk replacer diets for goat kids on protein, RNA, and DNA contents in small intestinal mucosa, on the importance of RNA biosynthesis from dietary RNA precursors for mucosal RNA synthesis, and on the activities of enzymes involved in nucleotide degradation in small intestinal mucosa. Diets were based on cow's milk. In the control group, 35% of the milk protein was replaced by casein (CN) protein, and in the soy group (SPAA), the same amount of milk protein was replaced by soy protein supplemented with essential AA known to be at lower concentrations in soy than in CN (Thr, Val, Ile, Leu, His, Lys, Met). Diets were isonitrogenous and isoenergetic. At 47 d of age, goats were harvested and samples of proximal, middle, and distal jejunal mucosa were collected 5 h after feeding 15N-labeled RNA from yeast (13 mg/kg of body weight). Growth and feed conversion did not differ between the control and SPAA kids. Mucosal protein concentrations were lower in the SPAA than the control kids. Concentrations of RNA and DNA did not differ between feeding groups, but in all kids mucosal RNA concentrations were higher in proximal than in middle and distal jejunum. Protein:RNA ratios were higher in the control than the SPAA kids and were lowest in proximal jejunum. Activities of alkaline phosphatase in enterocytes were higher in proximal than in middle and distal jejunum. Activities of mucosal xanthine oxidase were highest in distal jejunum and were higher in the SPAA than the control kids, especially in the middle and distal sites. The 15N-enrichment of mucosal RNA was higher in the control than the SPAA kids, especially in distal jejunum, and was lowest in distal jejunum. In contrast, 15N-enrichment of urea in plasma tended to be higher and Gly concentration in plasma was lower in the SPAA than the control kids. Data indicate that protein content and the protein:RNA ratio were lower in jejunal mucosa of goat kids fed milk replacer with partial replacement of CN protein by soy protein. These findings were accompanied by a lower level of reutilization of preformed dietary RNA precursors for RNA biosynthesis in jejunal mucosa and a higher activity of xanthine oxidase. Thus, feeding soy protein instead of CN protein reduced the incorporation of preformed dietary RNA precursors for RNA biosynthesis in the mucosa and activated key enzymes involved in nucleic acid breakdown.
Schönhusen
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Research Unit Nutritional Physiology Oskar Kellner, Research Institute for the Biology of Farm Animals (FBN), D-181196 Dummerstorf, Germany. schoenhu@fbn-dummerstorf.de
Kuhla
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Zitnan
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R
Wutzke
K D
KD
Huber
K
K
Moors
S
S
Voigt
J
J
D000596:Amino Acids / Q000008:administration & dosage / Q000032:analysis; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D001806:Blood Urea Nitrogen; D001835:Body Weight; D004032:Diet / Q000662:veterinary*; D004044:Dietary Proteins / Q000032:analysis / Q000378:metabolism; D004435:Eating; D005998:Glycine / Q000097:blood; D006041:Goats / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*; D007421:Intestine, Small / Q000378:metabolism*; D008297:Male; D041941:Milk Substitutes / Q000737:chemistry / Q000378:metabolism; D009584:Nitrogen / Q000097:blood / Q000378:metabolism; D012313:RNA / Q000032:analysis / Q000378:metabolism*; D030262:Soybean Proteins / Q000032:analysis / Q000378:metabolism*
D000596:Amino Acids; D004044:Dietary Proteins; D030262:Soybean Proteins; D012313:RNA; D009584:Nitrogen; D005998:Glycine