Transcriptional profiling approaches to understanding how plants regulate growth and defence: a case study illustrated by analysis of the role of vitamin C.
In this chapter, basic technical aspects concerning the design of DNA microarray experiments are discussed including sample preparation, hybridisation conditions and statistical significance of the acquired data are detailed. Given that microarrays are perhaps the most used tool in plant systems biology there is much experience in the pitfalls in using them. Herein important considerations are presented for both the experimental biologists and data analyst in order to maximise the utility of these resources. Finally a case study using the analysis of vitamin C deficient plants is presented to illustrate the power of this approach in enhancing comprehension of important and complex biological functions.
Foyer
Christine H
CH
Crop Performance and Improvement Division, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK. christine.foyer@bbsrc.ac.uk
Kiddle
Guy
G
Verrier
Paul
P
D001205:Ascorbic Acid / Q000378:metabolism*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D063245:Plant Development; D010944:Plants / Q000235:genetics* / Q000276:immunology; D014158:Transcription, Genetic
D001205:Ascorbic Acid