Pineapple juice and its fractions in enzymatic browning inhibition of banana [Musa (AAA group) Gros Michel].
The effectiveness of pineapple juice in enzymatic browning inhibition was evaluated on the cut surface of banana slices. After storage of banana slices at 15 degrees C for 3 days, pineapple juice showed browning inhibition to a similar extent as 8 mM ascorbic acid but less than 4 mM sodium metabisulfite. Fractionation of pineapple juice by a solid-phase C18 cartridge revealed that the directly eluted fraction (DE fraction) inhibited banana polyphenol oxidase (PPO) about 100% when compared to the control. The DE fraction also showed more inhibitory effect than 8 mM ascorbic acid in enzymatic browning inhibition of banana puree during storage at 5 degrees C for 24 h. Further identification of the DE fraction by fractionation with ion exchange chromatography and confirmation using model systems indicated that malic acid and citric acid play an important role in the enzymatic browning inhibition of banana PPO.
Chaisakdanugull
Chitsuda
C
Department of Food Science and Technology, Faculty of Agro-industry, Kasetsart University, Bangkok 10900, Thailand.
Theerakulkait
Chockchai
C
Wrolstad
Ronald E
RE
D031230:Ananas / Q000737:chemistry*; D001628:Beverages / Q000032:analysis; D004156:Catechol Oxidase / Q000037:antagonists & inhibitors; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005638:Fruit / Q000737:chemistry*; D015416:Maillard Reaction / Q000187:drug effects*; D028521:Musa / Q000737:chemistry* / Q000201:enzymology
D004791:Enzyme Inhibitors; D004156:Catechol Oxidase