Magnetic targeting of rhodamine-labeled superparamagnetic liposomes to solid tumors: in vivo tracking by fibered confocal fluorescence microscopy.
Polyethylene glycol (PEG)ylated and rhodamine-labeled liposomes loaded with maghemite nanocrystals provide a novel nanoscaled hybrid system for magnetic targeting to solid tumors in possible combination with double in vivo imaging by fluorescence microscopy and magnetic resonance imaging (MRI). Human prostate adenocarcinoma tumors implanted in mice were used as a system model. A magnetic field gradient was produced at the tumor level by external apposition of a magnet. Noninvasive fibered confocal fluorescence microscopy was successfully used to track the liposomes in vivo within organs and tumor blood vessels. Active targeting to the magnet-exposed tumors was clearly shown, in agreement with previous MRI studies. The liposomes were driven and accumulated within the microvasculature through a process that preserved vesicle structure and content.
Martina
Marie-Sophie
MS
Laboratoire de physico-chimie des systemes polyphases, UMR CNRS 8612, Université Paris-Sud, Faculté de pharmacie, F-92296 Châtenay-Malabry cedex, France.
Fortin
Jean-Paul
JP
Fournier
Laure
L
Ménager
Christine
C
Gazeau
Florence
F
Clément
Olivier
O
Lesieur
Sylviane
S
D000230:Adenocarcinoma / Q000175:diagnosis* / Q000378:metabolism; D000818:Animals; D052203:Ferrosoferric Oxide / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D005336:Fiber Optic Technology / Q000379:methods*; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D008297:Male; D053768:Metal Nanoparticles / Q000032:analysis* / Q000737:chemistry; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D011092:Polyethylene Glycols / Q000737:chemistry; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D012235:Rhodamines / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism
D005456:Fluorescent Dyes; D008081:Liposomes; D012235:Rhodamines; D011092:Polyethylene Glycols; D052203:Ferrosoferric Oxide