Detection of cathepsin S cysteine protease in human brain tumour microdialysates in vivo.
Microdialysis enables the chemistry of extracellular fluid in body tissues to be measured. Extracellular proteases such as the cysteine protease, cathepsin S (CatS), are thought to facilitate astrocytoma invasion. Microdialysates obtained from human brain tumours in vivo were subjected to cathepsin S activity and ELISA assays. Cathepsin S ELISA expression was detected in five out of 10 tumour microdialysates, while activity was detected in five out of 11 tumour microdialysates. Cathepsin S expression was also detected in microdialysate from the normal brain control although no activity was found in the same sample. While some refinements to the technique are necessary, the authors demonstrate the feasibility and safety of microdialysis in human astrocytomas in vivo. Characterisation of the extracellular environment of brain tumours in vivo using microdialysis may be a useful tool to identify the protease profile of brain tumours.
Flannery
T
T
Department of Neurosurgery, Royal Victoria Hospital, Belfast, Northern Ireland, UK.
McConnell
R S
RS
McQuaid
S
S
McGregor
G
G
Mirakhur
M
M
Martin
L
L
Scott
C
C
Burden
R
R
Walker
B
B
McGoohan
C
C
Johnston
P G
PG
D001254:Astrocytoma / Q000201:enzymology*; D001932:Brain Neoplasms / Q000201:enzymology*; D002403:Cathepsins / Q000032:analysis / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007150:Immunohistochemistry; D017551:Microdialysis / Q000379:methods*; D009363:Neoplasm Proteins / Q000032:analysis / Q000378:metabolism*; D010865:Pilot Projects
D009363:Neoplasm Proteins; D002403:Cathepsins; C028217:cathepsin S