Interaction of the protein C activation peptide with platelets.
The peptide NH(2)-DTEDQEDQVDPR-COOH is released during activation of protein C zymogen. We measured the effect of a synthetic peptide with an amino acid sequence similar to that of the natural peptide on platelets from healthy individuals using platelet aggregometry. We found that this synthetic peptide inhibits platelet aggregation induced by thrombin; furthermore, it diminishes mobilization of intraplatelet calcium. Molecular docking showed weak interaction between the synthetic peptide and thrombin. Our findings suggest that this synthetic peptide may interact with a receptor located on the platelet cell membrane.
Martínez-Cruz
Ruth
R
Centro de Investigación en Ciencias Médicas y Biológicas, Facultad de Medicina y Cirugía, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca, México.
Canseco
María Del S Pina
Mdel S
Lopez-Martínez
Jael
J
Cruz
Pedro A Hernández
PA
Pérez-Campos
Eduardo
E
Cruz
Margarito Martínez
MM
Alva
Félix Córdoba
FC
Majluf-Cruz
Abraham
A
Zenteno
Edgar
E
Ruiz-Argüelles
Alejandro
A
D000595:Amino Acid Sequence; D001792:Blood Platelets / Q000737:chemistry / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D004792:Enzyme Precursors / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism*; D015539:Platelet Activation / Q000187:drug effects*; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011956:Receptors, Cell Surface / Q000378:metabolism; D018179:Receptors, Thrombin / Q000378:metabolism; D013917:Thrombin / Q000494:pharmacology*
D004792:Enzyme Precursors; D009842:Oligopeptides; D010975:Platelet Aggregation Inhibitors; D011956:Receptors, Cell Surface; D018179:Receptors, Thrombin; C047135:protein C activator peptide; D013917:Thrombin; D002118:Calcium