Evaluation of immunoassay-based MMP-8 detection in gingival crevicular fluid on a point-of-care platform.
A novel immunology-based point-of-care test has been designed to assess the activated form of matrix metalloproteinase-8 (aMMP-8) for diagnosis and monitoring of periodontal diseases. The test has been automated using an analyzer, which quantitatively measures aMMP-8 in 18 min in gingival crevicular fluid (GCF). Fluid samples were collected from healthy, gingivitis-, and periodontitis-affected teeth. The test results from the analyzer were compared with quantitative aMMP-8 immunofluorometric assay (IFMA) and in-house enzyme-linked immunosorbent assay (ELISA) as well as with the periodontal state. Preliminary results of analyzer measurements of these 34 clinical samples showed a good agreement with the results from IFMA and in-house ELISA and with the clinical picture.
Munjal
S K
SK
Dentognostics GmbH, Jena, Germany. s.munjal@dentognostics.de
Prescher
N
N
Struck
F
F
Sorsa
T
T
Maier
K
K
Netuschil
L
L
D005883:Gingival Crevicular Fluid / Q000201:enzymology*; D006801:Humans; D007118:Immunoassay; D020784:Matrix Metalloproteinase 8 / Q000032:analysis* / Q000276:immunology; D010510:Periodontal Diseases / Q000175:diagnosis / Q000201:enzymology; D019095:Point-of-Care Systems
D020784:Matrix Metalloproteinase 8