Tumor suppressor proteins are differentially affected in human ependymoblastoma and medulloblastoma cells exposed to nerve growth factor.
The aim of our study was to investigate the role of nerve growth factor (NGF) on the expression of the p73 protein in human ependymoblastoma (EP) and medulloblastoma (MB) cells. It was found that NGF exposure on MB cells blocks proliferation, as well as on EP cells and induces overexpression of p73. NGF reduces the number of cells and promotes the expression of TrkA of these neoplastic cells. Moreover, NGF plus cisplatin treatment reduces the cytotoxic effect of cisplatin. These observations indicate that NGF by interfering with mechanisms associated with cells proliferation and survival might induce the differentiation event through TrkA pathways.
Antonelli
A
A
Institute of Neurobiology and Molecular Medicine, CNR-EBRI, Rome, Italy.
Lenzi
L
L
Nakagawara
A
A
Osaki
T
T
Chiaretti
A
A
Aloe
L
L
D000293:Adolescent; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival; D002675:Child, Preschool; D002945:Cisplatin; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006801:Humans; D008297:Male; D008527:Medulloblastoma / Q000378:metabolism* / Q000473:pathology; D020932:Nerve Growth Factor / Q000494:pharmacology*; D018242:Neuroectodermal Tumors, Primitive / Q000378:metabolism* / Q000473:pathology; D009687:Nuclear Proteins / Q000378:metabolism*; D020917:Receptor, trkA / Q000378:metabolism; D014407:Tumor Cells, Cultured; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins / Q000378:metabolism*
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C000606460:TP73 protein, human; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins; D020932:Nerve Growth Factor; D020917:Receptor, trkA; D002945:Cisplatin