title: Agrobacterium-mediated transformation of the dwarf pomegranate (Punica granatum L. var. nana).
abstract: The dwarf pomegranate (Punica granatum L. var. nana) is a dwarf ornamental plant that has the potential to be the model plant of perennial fruit trees because it bears fruits within 1 year of seedling. We established an Agrobacterium-mediated transformation system for the dwarf pomegranate. Adventitious shoots regenerated from leaf segments were inoculated with A. tumefaciens strain EHA105 harboring the binary vector pBin19-sgfp, which contains neomycin phosphotransferase (npt II) and green fluorescent protein (gfp) gene as a selectable and visual marker, respectively. After co-cultivation, the inoculated adventitious shoots were cut into small pieces to induce regeneration, and then selected on MS medium supplemented with 0.5 muM alpha-naphthaleneacetic acid (NAA), 5 muM N(6)-benzyladenine (BA), 0.3% gellan gum, 50 mg/l kanamycin, and 10 mg/l meropenem. Putative transformed shoots were regenerated after 6-8 months of selection. PCR and PCR-Southern blot analysis revealed the integration of the transgene into the plant genome. Transformants bloomed and bore fruits within 3 months of being potted, and the inheritance of the transgene was confirmed in T(1) generations. The advantage of the transformation of dwarf pomegranate was shown to be the high transformation rate. The establishment of this transformation system is invaluable for investigating fruit-tree-specific phenomena.
authors: University of Tsukuba, Tsukuba, Japan.
authors: Shingo
authors: S
authors: Terakami
authors: Nagao
authors: N
authors: Matsuta
authors: Toshiya
authors: T
authors: Yamamoto
authors: Sumiko
authors: S
authors: Sugaya
authors: Hiroshi
authors: H
authors: Gemma
authors: Junichi
authors: J
authors: Soejima
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001483:Base Sequence; D018744:DNA, Plant / Q000235:genetics; D004202:Disinfectants / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005638:Fruit; D029561:Lythraceae / Q000235:genetics*; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000033:anatomy & histology / Q000187:drug effects / Q000235:genetics; D030821:Plants, Genetically Modified; D012231:Rhizobium / Q000235:genetics* / Q000502:physiology*; D012641:Selection, Genetic; D014170:Transformation, Genetic
chemical_list: D000900:Anti-Bacterial Agents; D018744:DNA, Plant; D004202:Disinfectants