The effect of temperature, acidification, and alkalization changes as well as ethanol on salivary cathepsin D activity.
The activity of salivary cathepsin D undergoes inactivation at the temperature of 50-60 degrees C and at pH of 2.0 and pH of 8.0-10.0. The enzyme activity is also decreased by high concentrations of ethanol and high-proof alcoholic beverages. The factors should be taken into consideration in the evaluation of salivary cathepsin D activity.
Karwowska
A
A
Department of Instrumental Analysis, Medical University of Białystok, Poland. analist@amb.edu.pl
Roszkowska-Jakimiec
W
W
Dabrowska
E
E
Gacko
M
M
Chlabicz
M
M
D000143:Acids / Q000494:pharmacology; D000468:Alkalies / Q000494:pharmacology; D002402:Cathepsin D / Q000032:analysis* / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D012463:Saliva / Q000201:enzymology*; D013696:Temperature
D000143:Acids; D000468:Alkalies; D000431:Ethanol; D002402:Cathepsin D