Enzymes that hydrolyze adenine nucleotides of patients with hypercholesterolemia and inflammatory processes.
The activity of NTPDase (EC 3.6.1.5, apyrase, CD39) was verified in platelets from patients with increasing cholesterol levels. A possible association between cholesterol levels and inflammatory markers, such as oxidized low-density lipoprotein, highly sensitive C-reactive protein and oxidized low-density lipoprotein autoantibodies, was also investigated. Lipid peroxidation was estimated by measurement of thiobarbituric acid reactive substances in serum. The following groups were studied: group I, < 150 mg.dL(-1) cholesterol; group II, 151-200 mg.dL(-1) cholesterol; group III, 201-250 mg.dL(-1) cholesterol; and group IV, > 251 mg.dL(-1) cholesterol. The results demonstrated that both ATP hydrolysis and ADP hydrolysis were enhanced as a function of cholesterol level. Low-density lipoprotein levels increased concomitantly with total cholesterol levels. Triglyceride levels were increased in the groups with total cholesterol above 251 mg.dL(-1). Oxidized low-density lipoprotein levels were elevated in groups II, III, and IV. Highly sensitive C-reactive protein was elevated in the group with cholesterol levels higher than 251 mg.dL(-1). Oxidized low-density lipoprotein autoantibodies were elevated in groups III and IV. Thiobarbituric acid reactive substance content was enhanced as a function of cholesterol level. In summary, hypercholesterolemia is associated with enhancement of inflammatory response, oxidative stress, and ATP and ADP hydrolysis. The increased ATP and ADP hydrolysis in group IV was confirmed by an increase in CD39 expression on its surface. The increase in CD39 activity is possibly related to a compensatory response to the inflammatory and pro-oxidative state associated with hypercholesterolemia.
Duarte
Marta Medeiros Frescura
MM
Departamento de Química, Centro de Ciências Naturais e Exatas, Programa de Pos-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, Av. Roraima 1000, 97105-900 Santa Maria, RS, Brazil.
Loro
Vânia L
VL
Rocha
João B T
JB
Leal
Daniela B R
DB
Bem
Andreza F de
AF
Dorneles
Aracéli
A
Morsch
Vera M
VM
Schetinger
Maria R C
MR
D000227:Adenine Nucleotides / Q000378:metabolism*; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000097:blood*; D001081:Apyrase / Q000097:blood*; D001323:Autoantibodies / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism; D001792:Blood Platelets / Q000378:metabolism*; D002097:C-Reactive Protein / Q000032:analysis; D005260:Female; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood / Q000201:enzymology*; D007249:Inflammation / Q000097:blood / Q000201:enzymology*; D015227:Lipid Peroxidation; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood / Q000276:immunology; D008297:Male; D008875:Middle Aged; D014280:Triglycerides / Q000097:blood
D000227:Adenine Nucleotides; D015703:Antigens, CD; D001323:Autoantibodies; D001786:Blood Glucose; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D014280:Triglycerides; C099252:oxidized low density lipoprotein; D002097:C-Reactive Protein; D001081:Apyrase; C067869:CD39 antigen