title: Effects of temperature, storage period and the number of individuals on the detection of the false spider mite Cardinium endosymbiont.
abstract: Cardinium have been found as endosymbionts of Brevipalpus phoenicis, the mite vector of the Citrus leprosis virus. With the long-term objective being to understand the mechanisms of plant-virus-vector interactions, we evaluated the different storage conditions and periods, as well as the number of mites needed for PCR-amplification of such endosymbionts, making it possible to collect mites in different geographical regions without prolonged storage compromising subsequent analyses.
authors: Laboratório de Biotecnologia, Centro APTA Citros "Sylvio Moreira"-IAC, Rodovia Anhanguera Km 158, P.O. Box 04, 13490-970, Cordeirópolis, SP, Brazil. valdenice@centrodecitricultura.br
authors: Valdenice M
authors: VM
authors: Novelli
authors: Juliana
authors: J
authors: Freitas-Astúa
authors: Fernanda
authors: F
authors: Arrivabem
authors: Eliane C
authors: EC
authors: Locali-Fabris
authors: Mark E
authors: ME
authors: Hilf
authors: Tim R
authors: TR
authors: Gottwald
authors: Marcos A
authors: MA
authors: Machado
mesh_terms: D000818:Animals; D041964:Cytophagaceae / Q000235:genetics*; D004275:DNA, Ribosomal / Q000032:analysis*; D008925:Mites / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D018401:Sample Size; D013559:Symbiosis; D013696:Temperature; D013997:Time Factors
chemical_list: D004275:DNA, Ribosomal