L-argininamide improves the refolding more effectively than L-arginine.
L-arginine (Arg) is a widely used additive for suppressing protein aggregation during refolding. Systematic screening of Arg analogs provides superior additives that enhance the refolding yield more effectively than Arg. The refolding yield of hen egg lysozyme in the presence of 500 mM L-argininamide (ArgAd) increases 1.7-fold higher than Arg. Thermal unfolding experiments indicate that ArgAd has a greater denaturing effect than Arg. The refolding yield positively relates to the net charge of Arg analogs. Moreover ArgAd was also effective for the refolding of bovine carbonic anhydrase. High potency to increase the refolding yield of ArgAd compared to Arg results from high positive net charge and the denaturing property.
Hamada
Hiroyuki
H
Institute of Applied Physics, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8573, Japan.
Shiraki
Kentaro
K
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D002256:Carbonic Anhydrases; D002417:Cattle; D002645:Chickens; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D015394:Molecular Structure; D009113:Muramidase; D011489:Protein Denaturation; D017510:Protein Folding; D020673:Protein Renaturation / Q000187:drug effects
C069339:argininamide; D001120:Arginine; C421864:hen egg lysozyme; D009113:Muramidase; D002256:Carbonic Anhydrases