Comparative studies of differential expression of chitinolytic enzymes encoded by chiA, chiB, chiC and nagA genes in Aspergillus nidulans.
N-Acetyl-D-glucosamine, chito-oligomers and carbon starvation regulated chiA, chiB, and nagA gene expressions in Aspergillus nidulans cultures. The gene expression patterns of the main extracellular endochitinase ChiB and the N-acetyl-beta-D-glucosaminidase NagA were similar, and the ChiB-NagA enzyme system may play a morphological and/or nutritional role during autolysis. Alterations in the levels of reactive oxygen species or in the glutathione-glutathione disulfide redox balance, characteristic physiological changes developing in ageing and autolyzing fungal cultures, did not affect the regulation of either the growth-related chiA or the autolysis-coupled chiB genes although both of them were down-regulated under diamide stress. The transcription of the chiC gene with unknown physiological function was repressed by increased intracellular superoxide concentration.
Pusztahelyi
T
T
Department of Microbial Biotechnology and Cell Biology, Faculty of Science, University of Debrecen, Debrecen, Hungary. pusztahelyi@yahoo.com
Molnár
Z
Z
Emri
T
T
Klement
E
E
Miskei
M
M
Kerékgyárto
J
J
Balla
J
J
Pócsi
I
I
D000117:Acetylglucosamine / Q000494:pharmacology; D001233:Aspergillus nidulans / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D002244:Carbon / Q000378:metabolism; D002688:Chitinases / Q000096:biosynthesis* / Q000235:genetics; D019295:Computational Biology; D004271:DNA, Fungal / Q000235:genetics; D004790:Enzyme Induction; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D009844:Oligosaccharides / Q000494:pharmacology; D010084:Oxidation-Reduction; D010802:Phylogeny; D017382:Reactive Oxygen Species / Q000378:metabolism
D004271:DNA, Fungal; D005656:Fungal Proteins; D009844:Oligosaccharides; D017382:Reactive Oxygen Species; D002244:Carbon; D002688:Chitinases; D000117:Acetylglucosamine