Reactivation of latent human immunodeficiency virus 1 by human herpesvirus 6 infection.
Infection of the ACH-2 line of human leukemic T cells carrying latent Human immunodeficiency virus 1 (HIV-1) with Human herpesvirus 6 (HHV-6) resulted in an increase in reverse transcriptase (RT) activity, a marker of HIV-1 activation, in the culture supernatant. A similar effect was obtained with 12-O-tetradecanoyl-phorbol-13-acetate (TPA). The RT activity reached a peak at 24 hrs post infection (p.i.) and then declined, suggesting that the cells underwent lysis. The HIV-1 antigen was co-expressed with an early-late HHV-6 product, but not always with an immediate-early (IE) HHV-6 product, suggesting that one or more IE gene products were involved in the activation of latent HIV-1 in ACH-2 cells.
Isegawa
Y
Y
Graduate School of Medicine, Osaka University, Osaka, Japan. isegawa@bact.med.osaka-u.ac.jp
Katahira
J
J
Yamanishi
K
K
Sugimoto
N
N
D045744:Cell Line, Tumor; D015488:HIV Antigens / Q000096:biosynthesis; D054303:HIV Reverse Transcriptase / Q000032:analysis; D015497:HIV-1 / Q000502:physiology*; D015654:Herpesvirus 6, Human / Q000254:growth & development*; D006801:Humans; D008856:Microscopy, Fluorescence; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014775:Virus Activation; D017735:Virus Latency
D015488:HIV Antigens; D054303:HIV Reverse Transcriptase; D013755:Tetradecanoylphorbol Acetate