title: Involvement of cellular prion protein in the nociceptive response in mice.
abstract: The role of the cellular prion protein (PrP(c)) in neuronal functioning includes neuronal excitability, cellular adhesion, neurite outgrowth and maintenance. Here we investigated the putative involvement of the PrP(c) function on the nociceptive response using PrP(c) null (Prnp(0/0)) and wild-type (Prnp(+/+)) mice submitted to thermal and chemical models of nociception. PrP(c) null mice were more resistant than wild-type mice to thermal nociception of the tail-flick test. However, no significant difference was found on the hot plate test. In the acetic acid-induced visceral nociception, PrP(c) null mice showed an enhanced response when compared to wild-type mice. However, there was no difference between Prnp(0/0) and wild-type mice on glutamate- and formalin-induced licking behaviour and Freund's Complete Adjuvant (FCA)-induced mechanical allodynia. PrP(c) null mice developed significantly lower paw edema than wild-type mice. In addition, the visceral conditioning stimuli produced by a previous injection of acetic acid (20 days before testing) significantly reduced early and late phases of formalin-induced nociception in wild-type mice. In contrast, the same pre-treatment did not alter the formalin response in PrP(c) null mice. These results indicate a role of PrP(c) in the nociceptive transmission, including the thermal tail-flick test and visceral inflammatory nociception (acetic acid-induced abdominal constriction). Our findings show that PrP(c) is involved with a response mediated by inflammation (paw edema) and by visceral conditioning stimuli.
authors: Departamento de Química, Universidade Federal de Santa Maria, Santa Maria, RS, 97110-000, Brasil.
authors: Flavia Carla
authors: FC
authors: Meotti
authors: Cristiane Lima
authors: CL
authors: Carqueja
authors: Vinícius de Maria
authors: Vde M
authors: Gadotti
authors: Carla I
authors: CI
authors: Tasca
authors: Roger
authors: R
authors: Walz
authors: Adair R S
authors: AR
authors: Santos
mesh_terms: D019342:Acetic Acid / Q000009:adverse effects; D000704:Analysis of Variance; D000818:Animals; D001522:Behavior, Animal; D004487:Edema / Q000139:chemically induced; D005620:Freund's Adjuvant / Q000009:adverse effects; D018698:Glutamic Acid / Q000009:adverse effects; D006930:Hyperalgesia / Q000145:classification / Q000209:etiology / Q000235:genetics*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D010146:Pain / Q000209:etiology / Q000235:genetics* / Q000503:physiopathology*; D010147:Pain Measurement; D017288:Pain Threshold / Q000187:drug effects; D018621:PrPC Proteins / Q000172:deficiency / Q000502:physiology*; D011930:Reaction Time / Q000235:genetics; D013696:Temperature
chemical_list: D018621:PrPC Proteins; D018698:Glutamic Acid; D005620:Freund's Adjuvant; D019342:Acetic Acid