title: Relevance of combinatorial profiles of intermediate filaments and transcription factors for glioma histogenesis.
abstract: In order to define specific markers for histogenesis of three well-characterized subgroups of human gliomas (pilocytic astrocytomas, glioblastoma multiforme and oligodendrogliomas), we studied the expression of relevant markers that characterize gliomagenesis, by immunohistochemistry and in situ hybridization. They include the intermediate filament proteins glial fibrillary acidic protein (GFAP), vimentin and nestin, the transcription factors Olig2, Nkx2.2 and Sox10, and the proteolipid protein transcripts plp/dm20. We show that the three major categories of human gliomas express a combinatorial profile of markers that gives new insights to their histogenesis and may help diagnosis. Pilocytic astrocytomas strongly express GFAP, vimentin, Olig2, Nkx2.2 and Sox10 but not nestin. In contrast, glioblastomas strongly express GFAP, vimentin and nestin but these tumours are heterogeneous regarding the expression of the transcription factors studied. Finally, in oligodendrogliomas, intermediate filament proteins are generally not observed whereas Olig2 was found in almost all tumour cells nuclei while only a subpopulation of tumour cells expressed Nkx2.2 and Sox10.
authors: Laboratoire de Biopathologie de l'Adhésion et de la Signalisation, EA3281, IPHM, Faculté de Médecine Timone, Marseilles, France.
authors: C
authors: C
authors: Colin
authors: I
authors: I
authors: Virard
authors: N
authors: N
authors: Baeza
authors: A
authors: A
authors: Tchoghandjian
authors: C
authors: C
authors: Fernandez
authors: C
authors: C
authors: Bouvier
authors: A
authors: A
authors: Calisti
authors: S
authors: S
authors: Tong
authors: P
authors: P
authors: Durbec
authors: D
authors: D
authors: Figarella-Branger
mesh_terms: D000328:Adult; D000368:Aged; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics; D014408:Biomarkers, Tumor; D001932:Brain Neoplasms / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D004268:DNA-Binding Proteins / Q000235:genetics; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis / Q000235:genetics; D005910:Glioma / Q000235:genetics*; D006609:High Mobility Group Proteins / Q000235:genetics; D000090322:Homeobox Protein Nkx-2.2; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007381:Intermediate Filament Proteins / Q000096:biosynthesis / Q000235:genetics; D007382:Intermediate Filaments / Q000235:genetics*; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics; D064231:Nestin; D009687:Nuclear Proteins; D000076104:Oligodendrocyte Transcription Factor 2; D055757:SOXE Transcription Factors; D014157:Transcription Factors / Q000235:genetics*; D014746:Vimentin / Q000096:biosynthesis / Q000235:genetics; D029961:Zebrafish Proteins
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D005904:Glial Fibrillary Acidic Protein; D006609:High Mobility Group Proteins; D000090322:Homeobox Protein Nkx-2.2; D018398:Homeodomain Proteins; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; D064231:Nestin; D009687:Nuclear Proteins; D000076104:Oligodendrocyte Transcription Factor 2; D055757:SOXE Transcription Factors; D014157:Transcription Factors; D014746:Vimentin; D029961:Zebrafish Proteins; C577484:NES protein, human; C000715631:NKX2-2 protein, human; C405708:OLIG2 protein, human; C527923:SOX10 protein, human; C000715630:nkx2.2b protein, zebrafish