[Protein-protein interaction between hypothetical protein Rv1246c and Rv1247c of Mycobacterium tuberculosis].
OBJECTIVE
To investigate the protein-protein interaction between hypothetical protein Rv1246c and Rv1247c of Mycobacterium tuberculosis.


METHODS
By PCR technique, the complete open-reading frame sequences of Rv1246c and Rv1247c gene were amplified from the M. tuberculosis H37Rv genomic DNA as template. The PCR-amplified cDNAs of Rv1247c and Rv1246c gene were subcloned into pGBKT7 and pGADT7-Rec vector respectively for constructing recombinant plasmids pGBKT7-Rv1247c and pGADT7-Rv1246c. After verified by restriction endonuclease digestion and DNA sequence determination, the recombinant vectors were used to transform the yeast cell AH109 by lithium acetate method.


RESULTS
The yeast cells co-transformed with pGBKT7-Rv1247c and pGADT7-Rv1246c grew on SD/-Ade/-His/-Leu/-Trp plates, and the beta-galactosidase activity assays showed the positive signal.


CONCLUSIONS
The hypothetical protein Rv1246c and Rv1247c could interact with each other in yeast cells.
Shang
Zheng-Ling
ZL
Department of Infection and Immunity, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Bao
Lang
L
Yao
Su-Xia
SX
Zhang
Hui-Dong
HD
Li
Yan
Y
Zhong
Qi
Q
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D005822:Genetic Vectors / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D020798:Two-Hybrid System Techniques; D001616:beta-Galactosidase / Q000378:metabolism
D001426:Bacterial Proteins; D001616:beta-Galactosidase