title: Subcellular compartmentalisation of cadmium in white lupins determined by energy-dispersive X-ray microanalysis.
abstract: The microlocalisation of cadmium (Cd) at the tissue-cellular level in Lupinus albus L. cv. Multolupa was determined by energy-dispersive X-ray microanalysis (EDXMA). Experimental plants were grown on Cd-treated (0 and 150 microM) perlite for 35 days. In leaves, Cd was found inside cells (cytoplasm or vacuoles), especially in the vascular bundle cells. Cd-induced damage of the chloroplast structure was also detected. EDXMA of the roots showed the cell wall to be the main area of Cd binding at the cellular level; only a small amount of Cd was found in the vacuoles. At the tissue level, a decreasing Cd gradient was seen from the outer to the inner root cortical parenchyma. Cd and S were found co-localised in the vascular cylinder.
authors: Dpto Química Agrícola, Facultad de Ciencias, Universidad Autónoma de Madrid, 28049, Madrid, Spain.
authors: Saul
authors: S
authors: Vazquez
authors: Mercedes
authors: M
authors: Fernandez-Pascual
authors: Beatriz
authors: B
authors: Sanchez-Pardo
authors: Ramon O
authors: RO
authors: Carpena
authors: Pilar
authors: P
authors: Zornoza
mesh_terms: D002104:Cadmium / Q000378:metabolism*; D002736:Chloroplasts / Q000648:ultrastructure; D004577:Electron Probe Microanalysis; D031297:Lupinus / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D018515:Plant Leaves / Q000648:ultrastructure; D018517:Plant Roots / Q000648:ultrastructure
chemical_list: D002104:Cadmium