title: Curcuma longa extract as a histological dye for collagen fibres and red blood cells.
abstract: Crude ethanolic extract and column chromatographic fractions of the Allepey cultivar of Curcuma longa Roxb, commonly called turmeric (tumeric) in commerce, were used as a stain for tissue sections. Staining was carried out under basic, acidic and neutral media conditions. Inorganic and organic dissolution solvents were used. The stain was used as a counterstain after alum and iron haematoxylins. C. longa stained collagen fibres, cytoplasm, red blood cells and muscle cells yellow. It also stained in a fashion similar to eosin, except for its intense yellow colour. Preliminary phytochemical evaluation of the active column fraction revealed that it contained flavonoids, free anthraquinone and deoxy sugar. A cheap, natural dye can thus be obtained from C. longa.
authors: Department of Histopathology, School of Medical Laboratory Science, Obafemi Awolowo University Teaching Hospital, Ile-Ife, Nigeria. avwiorog@yahoo.com
authors: O G
authors: OG
authors: Avwioro
authors: S K
authors: SK
authors: Onwuka
authors: J O
authors: JO
authors: Moody
authors: J M
authors: JM
authors: Agbedahunsi
authors: T
authors: T
authors: Oduola
authors: O E
authors: OE
authors: Ekpo
authors: A A
authors: AA
authors: Oladele
mesh_terms: D004396:Coloring Agents; D030024:Curcuma; D003593:Cytoplasm / Q000648:ultrastructure; D004912:Erythrocytes / Q000166:cytology*; D024022:Fibrillar Collagens / Q000648:ultrastructure*; D006801:Humans; D032342:Muscle Cells / Q000166:cytology; D010936:Plant Extracts; D013194:Staining and Labeling / Q000379:methods
chemical_list: D004396:Coloring Agents; D024022:Fibrillar Collagens; D010936:Plant Extracts; C012596:turmeric extract