PACAP 38 inhibits adiponectin release.
BACKGROUND
Pituitary adenylate cyclase activating peptide (PACAP 38) is a neuropeptide with anti-inflammatory activity. Vasoactive intestinal peptide (VIP)/PACAP receptors are found in immune cells, endocrine glands and also in adipose tissue. Adiponectin is an adipocyte-derived protein hormone which possesses anti-inflammatory, antidiabetic and antiatherogenic properties. The aim of this study was to examine the influence of PACAP 38 on adiponectin release in basal conditions and during lipopolysaccharide (LPS)-induced acute inflammation.


METHODS
Male Wistar-Kyoto rats were divided into four groups which received intraperitoneal injections of 0.9% NaCl, LPS, PACAP 38 or LPS+PACAP 38, respectively. Serum adiponectin concentrations were measured using an ELISA test.


RESULTS
LPS administration did not change adiponectin concentration; however, PACAP 38 administered alone decreased serum adiponectin concentration after 2 h (p<0.05) and 4 h (p<0.01). In the group that received LPS+PACAP38, compared with LPS alone, no difference in adiponectin concentration was observed.


CONCLUSIONS
We conclude that PACAP 38 may directly modulate adiponectin secretion by adipocytes in basal conditions.
Bik
Wojciech
W
Neuroendocrinology Department, Medical Centre of Postgraduate Education, Warsaw, Poland. zncmkp@op.pl
Wolinska-Witort
Ewa
E
Baranowska-Bik
Agnieszka
A
Martynska
Lidia
L
Chmielowska
Magdalena
M
Baranowska
Boguslawa
B
D052242:Adiponectin / Q000097:blood / Q000378:metabolism*; D000818:Animals; D007249:Inflammation / Q000139:chemically induced; D007274:Injections, Intraperitoneal; D008070:Lipopolysaccharides; D008297:Male; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D011921:Rats, Inbred WKY
D052242:Adiponectin; D008070:Lipopolysaccharides; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide