title: An experimentally derived database of candidate Ras-interacting proteins.
abstract: We used a TAP-tag approach to identify candidate binding proteins for the related Ras family GTPases: H-Ras, R-Ras, and Rap1A. Protein complexes were isolated from mouse fibroblasts, and component proteins were identified by a combination of nanoflow HPLC and tandem mass spectrometry. H-Ras was found to associate with numerous cytoskeletal proteins including talin-1. R-Ras and Rap1A each associated with various signaling molecules, many of which are membrane-associated. Thus, we have established the first database of potential Ras interactors in mammalian cells.
authors: Division of Rheumatology, Department of Medicine, University of California, San Diego, La Jolla, California 92093, USA. lgoldfinger@ucsd.edu
authors: Lawrence E
authors: LE
authors: Goldfinger
authors: Celeste
authors: C
authors: Ptak
authors: Erin D
authors: ED
authors: Jeffery
authors: Jeffrey
authors: J
authors: Shabanowitz
authors: Jaewon
authors: J
authors: Han
authors: Jacob R
authors: JR
authors: Haling
authors: Nicholas E
authors: NE
authors: Sherman
authors: Jay W
authors: JW
authors: Fox
authors: Donald F
authors: DF
authors: Hunt
authors: Mark H
authors: MH
authors: Ginsberg
mesh_terms: D000818:Animals; D016466:CHO Cells; D002846:Chromatography, Affinity / Q000379:methods*; D006224:Cricetinae; D003412:Cricetulus; D030562:Databases, Protein; D051379:Mice; D041681:NIH 3T3 Cells; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020827:rap1 GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D018631:ras Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D011993:Recombinant Fusion Proteins; D020827:rap1 GTP-Binding Proteins; D018631:ras Proteins