Efficacy of transgenic rice expressing Cry1Ac and CpTI against the rice leaffolder, Cnaphalocrocis medinalis (Guenée).
Detached leaf bioassays, open field tests and cage tests were conducted to evaluate the control efficacy of two transgenic rice lines, expressing Cry1Ac and CpTI, against Cnaphalocrocis medinalis (Guenée) during 2005-2006 in Fuzhou, China. Bioassay results showed that cumulative feeding areas of C. medinalis on transgenic lines were significantly lower than that on control rice lines at different developmental stages. The corrected mortalities at 96 h after infestation on transgenic lines during six rice growth stages were >90% and 100% during experiments conducted in 2005 and 2006, respectively. In the open field test, there was no significant difference in egg density between transgenic and control lines during early days of infestation, but significant differences were detected in late season, due to serious damage on control lines. Larval densities on control lines were significantly higher than the low larval populations observed on transgenic lines during both seasons. The percentages of plants with folded leaves and percentages of folded leaves on transgenic lines were significantly lower than that on control lines with and without insecticide applications, during the entire season. In cage tests the cumulative numbers of C. medinalis adults derived from transgenic lines were significantly lower than that from control lines with and without insecticide treatments. The high level of efficacy of the two transgenic rice lines against C. medinalis may provide an important basis for reduced insecticide applications, an expansion of alternative pest-control strategies and insect resistance management of Bt rice in the future.
Han
Lanzhi
L
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, PR China.
Wu
Kongming
K
Peng
Yufa
Y
Wang
Feng
F
Guo
Yuyuan
Y
D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics; D004731:Endotoxins / Q000235:genetics; D006460:Hemolysin Proteins / Q000235:genetics; D007814:Larva / Q000502:physiology; D009036:Moths / Q000502:physiology*; D012275:Oryza / Q000235:genetics* / Q000382:microbiology*; D010572:Pest Control, Biological / Q000379:methods*; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000382:microbiology*; D014361:Trypsin Inhibitors / Q000235:genetics
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D014361:Trypsin Inhibitors; C030956:insecticidal crystal protein, Bacillus Thuringiensis