Exposure to acute physical and psychological stress alters the response of rat macrophages to corticosterone, neuropeptide Y and beta-endorphin.
The objective of the present study was to investigate the effect of acute exposure to electric tail shock stress (ES) and a stress witnessing procedure (SW), as models for physical and psychological stress paradigms, respectively on adherence, phagocytosis and hydrogen peroxide (H(2)O(2)) release from rat peritoneal macrophages. In addition, we studied the in vitro effects of corticosterone (CORT), neuropeptide Y (NPY) and beta-endorphin (BE) on adherence, phagocytosis and H(2)O(2) release from macrophages isolated from control rats and from rats that had been exposed to ES or SW procedures 24 h earlier. ES and SW comparably diminished phagocytosis and H(2)O(2) release, but did not influence macrophage adherence. In vitro treatment with CORT and NPY notably suppressed phagocytosis and potentiated H(2)O(2) release from macrophages. BE suppressed both phagocytosis and H(2)O(2) release from macrophages. Previous exposure to ES and SW altered the responsiveness of the isolated macrophages to their in vitro treatment with mediators of stress, making the cells less sensitive to the influence of CORT and NPY and to a lesser extent to BE. It could be concluded that changes in the local macrophage milieu induced by ES and SW 24 h earlier modify macrophage responses to subsequent in vitro exposure to the stress mimics, CORT, NPY and BE.
Stanojević
Stanislava
S
Institute of Immunology and Virology Torlak, Immunology Research Centre Branislav Jankovic, Vojvode Stepe 458, Belgrade, Serbia. canac@EUnet.yu
Mitić
Katarina
K
Vujić
Vesna
V
Kovacević-Jovanović
Vesna
V
Dimitrijević
Mirjana
M
D000208:Acute Disease; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D003345:Corticosterone / Q000494:pharmacology*; D004597:Electroshock; D006861:Hydrogen Peroxide / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009478:Neuropeptide Y / Q000494:pharmacology*; D010587:Phagocytosis / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013312:Stress, Physiological / Q000209:etiology / Q000473:pathology / Q000503:physiopathology*; D013315:Stress, Psychological / Q000209:etiology / Q000473:pathology / Q000503:physiopathology*; D013623:Tail; D001615:beta-Endorphin / Q000494:pharmacology*
D009478:Neuropeptide Y; D001615:beta-Endorphin; D006861:Hydrogen Peroxide; D003345:Corticosterone