title: Flow photolysis for spatiotemporal stimulation of single cells.
abstract: Quantitative studies of cellular systems require experimental techniques that can expose single cells to well-controlled chemical stimuli with high spatiotemporal resolution. Here, we combine microfluidic techniques with the photochemical release of caged signaling molecules to generate tailored stimuli on the length scale of individual cells with subsecond switching times. We exemplify this flexible approach by initiating membrane translocation of fluorescent fusion proteins in chemotactic Dictyostelium discoideum cells.
authors: Max Planck Institute for Dynamics and Self-Organization, 37077 Göttingen, Germany.
authors: Carsten
authors: C
authors: Beta
authors: Danica
authors: D
authors: Wyatt
authors: Wouter-Jan
authors: WJ
authors: Rappel
authors: Eberhard
authors: E
authors: Bodenschatz
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002477:Cells / Q000187:drug effects*; D004023:Dictyostelium / Q000166:cytology; D005453:Fluorescence; D044085:Microfluidics / Q000379:methods*; D010782:Photolysis; D011993:Recombinant Fusion Proteins / Q000493:pharmacokinetics
chemical_list: D011993:Recombinant Fusion Proteins