title: [Molecular study of methicillin-resistant Staphylococcus haemolyticus in a Mexican hospital].
abstract: OBJECTIVE
  To perform the molecular characterization of methicillin-resistant Staphylococcus haemolyticus (MRSH) clinical isolates from patients in a Mexican hospital.
  
  
  METHODS
  Sixty three Staphylococcus ssp. isolates collected from September 2000 to October 2002 were analyzed. Antimicrobial susceptibility was determined by disk diffusion method and the presence of the mecA gene was detected by PCR technique. Isolates characterization was carried out by pulsed field gel electrophoresis (PFGE).
  
  
  RESULTS
  The frequency of S. haemolyticus was 25.5% (18 of 63 clinical isolates), all S. haemolyticus isolates were methicillin-resistant and they were positive for the mecA gene. A major pattern (A) with 8 subtypes was identified. This clone was distributed during the 20 months period. Most of them were isolated from the surgery (55%) and pediatric services (27.5%).
  
  
  CONCLUSIONS
  The methicillin-resistant S. haemolyticus permanence as pathogen in this hospital, suggest the implementation of control programs in order to decrease the prevalence of this multiresistant pathogen.
authors: Laboratorio de Investigaciones Microbiológicas, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero. natycastro2@hotmail.com
authors: Natividad
authors: N
authors: Castro
authors: María Salomé
authors: MS
authors: Loaiza-Loeza
authors: Amparo
authors: A
authors: Calderón-Navarro
authors: Alejandro
authors: A
authors: Sánchez
authors: Jesús
authors: J
authors: Silva-Sánchez
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D006761:Hospitals; D006801:Humans; D016106:Methicillin Resistance; D008800:Mexico; D041162:Staphylococcus haemolyticus / Q000187:drug effects* / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins