title: Characterization of complete genome sequence of the spring viremia of carp virus isolated from common carp (Cyprinus carpio) in China.
abstract: The complete genome of spring viraemia of carp virus (SVCV) strain A-1 isolated from cultured common carp (Cyprinus carpio) in China was sequenced and characterized. Reverse transcription-polymerase chain reaction (RT-PCR) derived clones were constructed and the DNA was sequenced. It showed that the entire genome of SVCV A-1 consists of 11,100 nucleotide base pairs, the predicted size of the viral RNA of rhabdoviruses. However, the additional insertions in bp 4633-4676 and bp 4684-4724 of SVCV A-1 were different from the other two published SVCV complete genomes. Five open reading frames (ORFs) of SVCV A-1 were identified and further confirmed by RT-PCR and DNA sequencing of their respective RT-PCR products. The 5 structural proteins encoded by the viral RNA were ordered 3'-N-P-M-G-L-5'. This is the first report of a complete genome sequence of SVCV isolated from cultured carp in China. Phylogenetic analysis indicates that SVCV A-1 is closely related to the members of the genus Vesiculovirus, family Rhabdoviridae.
authors: State Key Laboratory of Biocontrol, College of Life Sciences, Sun Yat-sen University, Guangzhou, P.R. China.
authors: Y
authors: Y
authors: Teng
authors: H
authors: H
authors: Liu
authors: J Q
authors: JQ
authors: Lv
authors: W H
authors: WH
authors: Fan
authors: Q Y
authors: QY
authors: Zhang
authors: Q W
authors: QW
authors: Qin
mesh_terms: D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000821:virology*; D002681:China; D005393:Fish Diseases / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D018353:Rhabdoviridae Infections / Q000662:veterinary* / Q000821:virology; D017422:Sequence Analysis, DNA; D018116:Vesiculovirus / Q000145:classification* / Q000235:genetics; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics; D014766:Viremia / Q000662:veterinary* / Q000821:virology
chemical_list: D014764:Viral Proteins