Assembly of DNA nanostructures with branched tris-DNA.
Branched tris-DNA, in which two oligonucleotides of the same sequence and one other oligonucleotide of a different sequence are connected with a rigid central linker, was prepared chemically by using a DNA synthesizer. Two branched tris-DNA molecules with complementary DNA sequences form dimer and tetramer as well as linear and spherical oligomer complexes. The complex formation was studied by UV/thermal denaturation, enzyme digestion, gel electrophoresis, and AFM imaging.
Kuroda
Takahiro
T
Department of Applied Chemistry, Faculty of Engineering, Gunma University, Kiryu, Gunma 376-8515, Japan24.
Sakurai
Yoshimasa
Y
Suzuki
Yukinao
Y
Nakamura
Akiko O
AO
Kuwahara
Masayasu
M
Ozaki
Hiroaki
H
Sawai
Hiroaki
H
D020029:Base Pairing; D004247:DNA / Q000737:chemistry*; D019281:Dimerization; D004587:Electrophoresis, Agar Gel; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D018625:Microscopy, Atomic Force; D008956:Models, Chemical; D049329:Nanostructures / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry; D012313:RNA / Q000737:chemistry; D013696:Temperature; D014325:Tromethamine / Q000737:chemistry*; D014466:Ultraviolet Rays
D009841:Oligonucleotides; D014325:Tromethamine; D012313:RNA; D004247:DNA