Fourier transform infrared imaging as a method for detection of HLA class I expression in melanoma without the use of antibody.
Human leukocyte antigen (HLA) class I expression in melanoma is usually assessed using immunohistochemical staining. Here we report on the use of Fourier transform infrared (FTIR) hyperspectral imaging, a method widely used in two-dimensional analysis of chemical components, to study HLA class I expression in tissue. Two-dimensional cluster colour images derived from unsupervised hierarchical cluster analysis of FTIR hyperspectral data on melanoma sections were compared with consecutive sections that were immunohistochemically stained for class I expression. HLA-class-I-positive and -negative areas were differentiated by FTIR cluster images in all eight melanoma sections investigated without the need for antibody attachment. FTIR imaging enables the distinction of HLA-class-I-positive from class-I-negative areas in melanoma. This method is accurate, rapid and cost-effective.
Chew
S F
SF
Centre for Biospectroscopy and School of Chemistry, Monash University, Victoria 3800, Australia.
Wood
B R
BR
Kanaan
C
C
Browning
J
J
MacGregor
D
D
Davis
I D
ID
Cebon
J
J
Tait
B D
BD
McNaughton
D
D
D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D006801:Humans; D008545:Melanoma / Q000175:diagnosis* / Q000378:metabolism; D012878:Skin Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared
D015395:Histocompatibility Antigens Class I