[Expression, purification, and characterization of an anti-human RBC ScFv-HIV gp160 fusion protein for hemagglutination-based rapid detection of antibodies to HIV in whole blood].
OBJECTIVE
To construct and express anti-human RBC and HIVgp160 fusion protein for rapid detection of antibody to HIV.


METHODS
The gene of the anti human RBC ScFv and HIV antigen were constructed together into expression vector. The fusion protein was expressed in E. coli.


RESULTS
The fusion protein was proved to be able to bind both anti-RBC and HIVgp160. It could cause agglutination of human RBC when HIVgp160 was present.


CONCLUSIONS
The fusion protein has the potential in rapid detection of HIV.
Hu
Yan
Y
Department of Virology, The No. 302 Hospital of the People's Liberation Army, Beijing, 100039, China.
Yang
Jian-yang
JY
Zhu
Lei
L
Hou
Jun
J
Shen
Hong-hui
HH
Mao
Pan-yong
PY
D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification; D001323:Autoantibodies / Q000276:immunology / Q000302:isolation & purification; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000276:immunology*; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D015483:HIV Antibodies / Q000097:blood* / Q000276:immunology; D019302:HIV Envelope Protein gp160 / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D006679:HIV Seropositivity / Q000097:blood; D006386:Hemagglutination Tests / Q000379:methods; D006801:Humans; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D015483:HIV Antibodies; D019302:HIV Envelope Protein gp160; D011993:Recombinant Fusion Proteins