title: Genetic characterization of canine rotavirus isolated from a puppy in Korea and experimental reproduction of disease.
abstract: Canine rotavirus was isolated from feces of a Korean Jindo dog with mild diarrhea, and the isolate was genetically characterized. Rotaviral antigen was detected in the feces using a commercial rotavirus antigen detection kit and cytopathic effects were observed in a cell line inoculated with the feces. The virus isolate (GC/KS05) was identified as subtype G3P[3] using reverse transcription polymerase chain reaction (RT-PCR). The strain displayed 98% and 90% identity with the VP7 genes of a canine rotavirus isolate (RV52/96) from Italy and the simian rotavirus strain (RRV) respectively. However, the GC/KS05 isolate exhibited only 83% and 82% identity, respectively, with the G3 serotype canine strains, RV198/95 and K9. Phylogenetic analysis of the VP7 and VP4 genes of GC/KS05 strain led to the classification of VP7 in a different cluster than other canine rotavirus VP7 genes, and VP4 within the cluster of canine rotavirus VP4 genes. The Korean isolate was thus more closely related to the RV52/96 isolate than the other isolates for which sequence data is available. Detailed analysis of the VP7 region revealed 6 amino acid variations between the new isolate and RV52/96. After 5 passages in cell culture, the GC/KS05 strain remained pathogenic for young pups, in which inoculation resulted in diarrhea and virus shedding in the feces.
authors: Department of Veterinary Medicine Virology Lab, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
authors: Bo Kyu
authors: BK
authors: Kang
authors: Dae Sub
authors: DS
authors: Song
authors: Kwon Il
authors: KI
authors: Jung
authors: Chul Seung
authors: CS
authors: Lee
authors: Sung Jun
authors: SJ
authors: Park
authors: Jin Sik
authors: JS
authors: Oh
authors: Dong Jun
authors: DJ
authors: An
authors: Jeong Sun
authors: JS
authors: Yang
authors: Hyoung Joon
authors: HJ
authors: Moon
authors: Sang Sun
authors: SS
authors: Lee
authors: Young Dhuk
authors: YD
authors: Yoon
authors: Bong Kyun
authors: BK
authors: Park
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D007723:Korea; D008969:Molecular Sequence Data; D010802:Phylogeny; D012401:Rotavirus / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000662:veterinary* / Q000821:virology
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; C050779:VP7 protein, Rotavirus