title: Resuscitation with hydroxyethyl starch solution prevents nuclear factor kappaB activation and oxidative stress after hemorrhagic shock and resuscitation in rats.
abstract: Fluid resuscitation is vital for treating traumatic hemorrhagic shock (HS), but reperfusion is believed to have the adverse consequences of generating reactive oxygen species and inflammatory cytokines, both of which cause multiple organ dysfunctions. We investigated the effects of various resuscitation fluids on the changes of redox-sensitive molecules after HS and fluid resuscitation (HS/R). We induced HS by bleeding male Sprague-Dawley rats to a blood pressure of 30 to 40 mmHg for 60 minutes. Thirty minutes later, the rats were killed (HS group) or immediately resuscitated with shed blood (HS + BL group), L-isomer lactated Ringer's solution (HS + LR group), or hydroxyethyl starch (HS + HES group). After HS, we found a significant increase in nuclear factor kappaB DNA binding activity, which was effectively inhibited using HES solution or blood resuscitation. Moreover, resuscitation with blood or LR solution, but not HES solution, induced significant oxidative stress, manifested by a high ratio of oxidized glutathione to reduced glutathione in the lungs, liver, and spleen. HS alone, however, did not increase the ratio of the oxidized glutathione to reduced glutathione in all organs. Although the protein expression of anti-apoptotic Bcl-2 and pro-apoptotic Bax varied in different organs, we found that resuscitation using HES solution prevented the HS-induced reduction of the Bcl-2/Bax ratio in the heart. HES solution was an appropriate resuscitation fluid in reversing nuclear factor kappaB activation, maintaining the Bcl-2/Bax ratio, and preventing oxidative stress after acute HS.
authors: Department of Emergency Medicine, National Cheng Kung University Hospital, Tainan, Taiwan.
authors: Ming-Che
authors: MC
authors: Tsai
authors: Wei-Ju
authors: WJ
authors: Chen
authors: Cheng-Hsin
authors: CH
authors: Ching
authors: Jih-Ing
authors: JI
authors: Chuang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D024202:Electrophoretic Mobility Shift Assay; D005440:Fluid Therapy / Q000379:methods; D005978:Glutathione / Q000378:metabolism; D019803:Glutathione Disulfide / Q000378:metabolism; D006895:Hydroxyethyl Starch Derivatives / Q000494:pharmacology / Q000627:therapeutic use*; D007552:Isotonic Solutions / Q000494:pharmacology / Q000627:therapeutic use; D008297:Male; D016328:NF-kappa B / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D010952:Plasma Substitutes / Q000494:pharmacology / Q000627:therapeutic use; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley; D012151:Resuscitation / Q000379:methods*; D000077325:Ringer's Lactate; D012771:Shock, Hemorrhagic / Q000378:metabolism / Q000628:therapy*; D051028:bcl-2-Associated X Protein / Q000378:metabolism; D051025:bcl-Associated Death Protein / Q000378:metabolism
chemical_list: D006895:Hydroxyethyl Starch Derivatives; D007552:Isotonic Solutions; D016328:NF-kappa B; D010952:Plasma Substitutes; D000077325:Ringer's Lactate; D051028:bcl-2-Associated X Protein; D051025:bcl-Associated Death Protein; D005978:Glutathione; D019803:Glutathione Disulfide