title: Transgenic Nicotiana benthamiana plants resistant to cucumber green mottle mosaic virus based on RNA silencing.
abstract: RNA silencing technology was used to confer resistance to cucumber green mottle mosaic virus (CGMMV). Nicotiana benthamiana was transformed with a transgene designed to produce an inverted repeat RNA containing CGMMV-coat protein gene (CP) sequences, which were separated by an intron sequence, under the control of the cauliflower mosaic virus 35S promoter. We attempted to confirm the resistance of seven independent transgenic lines; five lines showed resistance to CGMMV infection. The systemic spread of virus was prevented after the inoculation of CGMMV, and the CP-specific short interfering RNA (siRNA) was detected in resistant lines. Thus, the resistance against CGMMV through RNA silencing is strong and efficient.
authors: National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan.
authors: Shinichiro
authors: S
authors: Kamachi
authors: Atsuko
authors: A
authors: Mochizuki
authors: Masamichi
authors: M
authors: Nishiguchi
authors: Yutaka
authors: Y
authors: Tabei
mesh_terms: D036022:Capsid Proteins / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D010935:Plant Diseases / Q000235:genetics* / Q000821:virology*; D010942:Plant Viruses / Q000235:genetics*; D030821:Plants, Genetically Modified; D034622:RNA Interference; D014026:Nicotiana / Q000235:genetics* / Q000821:virology*; D019076:Transgenes / Q000235:genetics
chemical_list: D036022:Capsid Proteins