title: Enhanced yield of chromosome aberrations after CT examinations in paediatric patients.
abstract: OBJECTIVE
  To determine whether computed tomography (CT) could enhance the chromosome aberration yields in paediatric patients.
  
  
  METHODS
  Blood samples were taken before and after CT scans from 10 children for whom the medical justifications for CT examinations were accidental injuries and not diseases as investigated in earlier studies. Chromosome analysis was carried out in lymphocytes by fluorescence plus Giemsa (FPG) staining exclusively in metaphases of the first cell cycle in vitro.
  
  
  RESULTS
  The mean blood dose of the 10 children was about 12.9 mGy which was determined by a newly developed dose estimation. Based on more than 20,000 analyzed cells it was found that after CT examination the frequencies of dicentrics (dic) and excess acentric fragments (ace) in lymphocytes were significantly increased. By subdividing the children into two age groups, those with an age from 0.4 years to 9 years and from 10 - 15 years, it became obvious that the observed increase in chromosome aberrations was mainly contributed by the younger age group. In this group the frequency of dicentrics was significantly increased whereas in the older group the observed increase was not significant.
  
  
  CONCLUSIONS
  Our results demonstrate that CT examinations enhance the dicentrics yields in peripheral lymphocytes of children aged up to 15 years. Since in particular significantly increased dicentric yields could be observed in children with an age from 0.4 - 9 years, it can be assumed that children younger than 10 years may be more radiation sensitive than older subjects.
authors: Federal Office for Radiation Protection, Oberschleissheim, Germamy.
authors: G
authors: G
authors: Stephan
authors: K
authors: K
authors: Schneider
authors: W
authors: W
authors: Panzer
authors: L
authors: L
authors: Walsh
authors: U
authors: U
authors: Oestreicher
mesh_terms: D000293:Adolescent; D000367:Age Factors; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D002869:Chromosome Aberrations / Q000528:radiation effects*; D020732:Cytogenetic Analysis; D005260:Female; D006801:Humans; D007223:Infant; D008214:Lymphocytes / Q000166:cytology / Q000528:radiation effects*; D008297:Male; D008677:Metaphase / Q000528:radiation effects; D011829:Radiation Dosage; D014057:Tomography, X-Ray Computed / Q000009:adverse effects* / Q000379:methods; D014965:X-Rays / Q000009:adverse effects*