No association of factor V Leiden, prothrombin G20210A, and MTHFR C677T gene polymorphisms with kidney allograft survival: a multicenter study.
BACKGROUND
It has been proposed that inherited risk factors of venous thromboembolism, such as factor V G1691A (FV-Leiden), prothrombin G20210A, and methylenetetrahydrofolate reductase (MTHFR) C677T, might be associated with poorer survival rates of transplanted kidneys. On the basis of this hypothesis, we performed a multicenter study, involving recipients of primary and repeat kidney transplants, to investigate the potential effect of these three single nucleotide polymorphisms (SNP) on graft survival.


METHODS
The study consisted of 676 first and 651 retransplant patients. Using the polymerase chain reaction-sequence specific primers method, we typed all patients for the three SNP and analyzed graft survival.


RESULTS
We could not find a statistically significant association between graft survival and factor V Leiden or MTHFR C677T genotypes. A better 3-yr graft survival was found for first transplant recipients with the genotype prothrombin 20210 G/G as compared to those with the G/A genotype (P=0.031). However, Bonferroni correction for the three SNPs investigated in this series rendered the P value insignificant (P(corrected)=0.093).


CONCLUSIONS
We did not find a statistically significant association of SNP factor V Leiden G1691A and MTHFR C677T with renal graft survival. Prothrombin G20210A resulted in a significant association that was not sustained after Bonferroni correction. This SNP might be an interesting candidate for future studies.
Meyer
Marko
M
Department of Transplantation Immunology, University of Heidelberg, Heidelberg, Germany.
Laux
Gunter
G
Scherer
Sabine
S
Tran
Thuong Hien
TH
Opelz
Gerhard
G
Mytilineos
Joannis
J
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D003545:Cysteine / Q000235:genetics; D005165:Factor V / Q000235:genetics*; D005260:Female; D005838:Genotype; D005998:Glycine / Q000235:genetics; D006085:Graft Survival / Q000235:genetics*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D016030:Kidney Transplantation / Q000706:statistics & numerical data; D008297:Male; D045102:Methylenetetrahydrofolate Dehydrogenase (NAD+) / Q000235:genetics*; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics*; D011516:Prothrombin / Q000235:genetics*; D015996:Survival Rate; D013997:Time Factors; D014019:Tissue Donors
C095381:factor V Leiden; D005165:Factor V; D011516:Prothrombin; D045102:Methylenetetrahydrofolate Dehydrogenase (NAD+); D003545:Cysteine; D005998:Glycine