title: Effect of L-carnitine infusion and feed restriction on carnitine status in lactating Holstein cows.
abstract: Previously we determined that abomasal infusion of L-carnitine increased in vitro hepatic fatty acid oxidation, decreased liver lipid accumulation, and supported higher fat-corrected milk yield in feed-restricted lactating cows. The objectives of this study were to examine the effects of supplemental L-carnitine and amount of feed intake on free carnitine and carnitine ester concentrations in liver, muscle, milk, and plasma of lactating dairy cows. Eight lactating Holstein cows (132 +/- 36 d in milk) were used in a replicated 4 x 4 Latin square design with 14-d periods to test factorial combinations of water or L-carnitine infusion (20 g/d; d 5 to 14) and ad libitum or restricted (50% of previous 5-d intake; d 10 to 14) dry matter intake. Plasma was obtained 3 times daily on d 4, 8, and 12; milk samples were collected on d 8, 9, 13, and 14. Liver and muscle were biopsied on d 14 of each period. Free carnitine, short-chain acylcarnitine, and long-chain acylcarnitine concentrations were determined using a radioenzymatic assay coupled with ion exchange chromatography. Abomasal L-carnitine infusion increased total carnitine in plasma on d 8 and d 12. All liver carnitine fractions were increased by carnitine infusion. Feed restriction elevated concentrations of free carnitine, long-chain acylcarnitine, and total carnitine in liver tissue from carnitine-infused cows but not in those infused with water. In muscle, acid-soluble carnitine, long-chain acylcarnitine, and total carnitine concentrations were increased by carnitine infusion and feed restriction without significant interaction. Feed restriction increased free carnitine concentrations in muscle from water-infused cows but not in carnitine-infused cows. Carnitine infusion increased the concentration of each milk carnitine fraction as well as milk carnitine output on d 8 to 9. On d 13 to 14, all carnitine fractions except short-chain acylcarnitine were increased in milk from water-infused, feed-restricted cows, whereas all fractions were increased in carnitine-infused, feed-restricted cows. Carnitine infusion increased total carnitine in plasma, liver, muscle, and milk during feed restriction, whereas feed restriction alone increased carnitine concentrations in muscle and milk but not in liver. Liver carnitine concentrations might limit hepatic fatty acid oxidation capacity in dairy cows during the periparturient period; therefore, supplemental L-carnitine might decrease liver lipid accumulation in periparturient cows.
authors: Department of Animal Sciences, University of Illinois, Urbana 61801, USA.
authors: D B
authors: DB
authors: Carlson
authors: J C
authors: JC
authors: Woodworth
authors: J K
authors: JK
authors: Drackley
mesh_terms: D000018:Abomasum / Q000378:metabolism; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D002331:Carnitine / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D002417:Cattle / Q000502:physiology*; D005260:Female; D005508:Food Deprivation / Q000502:physiology*; D007774:Lactation / Q000502:physiology; D008099:Liver / Q000737:chemistry / Q000187:drug effects; D008892:Milk / Q000737:chemistry; D009132:Muscles / Q000737:chemistry / Q000187:drug effects; D014803:Vitamin B Complex / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*
chemical_list: D014803:Vitamin B Complex; D002331:Carnitine