Effects of low-level formaldehyde exposure on synaptic plasticity-related gene expression in the hippocampus of immunized mice.
We examined the effects of inhalative exposure to formaldehyde (FA, 400 ppb) on N-methyl-D-aspartate (NMDA) receptor subunits (NR2A and NR2B), dopamine receptor subtypes (D1 and D2), cyclic AMP responsive element-binding protein (CREB)-1, CREB-2, FosB/DeltaFosB, and transient receptor potential vanilloid receptor (TRPV1) in the hippocampus of ovalbumin-immunized mice using quantitative real-time PCR. Western blot analyses for pCREB were performed. The mRNA levels of NR2A, D1 and D2 receptors, and CREB-1 were significantly increased by FA, but NR2B, CREB-2, FosB/DeltaFosB, and TRPV1 mRNA levels remained unchanged. Treatment with MK-801 normalized the mRNA levels induced by FA. There was no significant effect of FA exposure and MK-801 treatment on the protein level of pCREB. These results indicate that FA exposure selectively up-regulates hippocampal gene expression in immunologically sensitized mice. The FA effects are presumably mediated by glutamatergic neurotransmission through NMDA receptors.
Ahmed
Sohel
S
Environmental Health Sciences Division, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba 305-8506, Ibaraki, Japan.
Tsukahara
Shinji
S
Tin-Tin-Win-Shwe
Yamamoto
Shoji
S
Kunugita
Naoki
N
Arashidani
Keiichi
K
Fujimaki
Hidekazu
H
D000818:Animals; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D016291:Dizocilpine Maleate / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005260:Female; D005404:Fixatives / Q000494:pharmacology*; D005557:Formaldehyde / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects; D007114:Immunization; D051379:Mice; D009473:Neuronal Plasticity / Q000187:drug effects*; D010047:Ovalbumin / Q000008:administration & dosage; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011954:Receptors, Dopamine / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013569:Synapses / Q000187:drug effects*; D050916:TRPV Cation Channels / Q000378:metabolism
C492004:Creb1 protein, mouse; D017362:Cyclic AMP Response Element-Binding Protein; D018691:Excitatory Amino Acid Antagonists; D005404:Fixatives; C483819:Fosb protein, mouse; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D011954:Receptors, Dopamine; D016194:Receptors, N-Methyl-D-Aspartate; D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse; D005557:Formaldehyde; D016291:Dizocilpine Maleate; D010047:Ovalbumin