title: Association between plasma BPDE-Alb adduct concentrations and DNA damage of peripheral blood lymphocytes among coke oven workers.
abstract: OBJECTIVE
  Coke oven emissions (COE) containing polycyclic aromatic hydrocarbons (PAHs) can induce both benzo[a]pyrene-r-7, t-8, t-9,c-10-tetrahydotetrol-albumin (BPDE-Alb) adducts and DNA damage. However, the relation between these biomarkers for early biological effects is not well documented in coke oven workers.
  
  
  METHODS
  In this study, the authors recruited 207 male workers exposed to COE and 102 controls not exposed to COE in the same steel plant in northern China. They measured BPDE-Alb adduct concentrations in plasma with reverse-phase high performance liquid chromatography and DNA damage in peripheral blood lymphocytes with alkaline comet assay.
  
  
  RESULTS
  The results showed that the median concentration of BPDE-Alb adducts in the exposed group (34.36 fmol/mg albumin) was significantly higher than that in the control group (21.90 fmol/mg albumin, p = 0.012). The mean Olive tail moment (Olive TM) of DNA damage in the exposed and control groups were 1.20 and 0.63, respectively (p = 0.000). Multivariate logistic regression analysis revealed that the odds ratio (OR) for BPDE-Alb adduct and Olive TM associated with the exposure were 1.72 (95% CI 1.06 to 2.81) and 1.96 (95% CI 1.20 to 3.19), respectively. These results show significant correlations between the concentrations of BPDE-Alb adduct and Olive TM levels in exposed group (r = 0.235, p = 0.001) but not in control group (r = 0.093, p = 0.353).
  
  
  CONCLUSIONS
  The results suggest that occupational exposure to COE may induce both BPDE-Alb adducts and DNA damage in the lymphocytes of coke oven workers and that these two markers are useful for monitoring exposure to COE in the workplace.
authors: Institute of Occupational Medicine and The Ministry of Education Key Lab for Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors: Hong
authors: H
authors: Wang
authors: Weihong
authors: W
authors: Chen
authors: Hongyan
authors: H
authors: Zheng
authors: Liang
authors: L
authors: Guo
authors: Huashan
authors: H
authors: Liang
authors: Xiaobo
authors: X
authors: Yang
authors: Yun
authors: Y
authors: Bai
authors: Jianya
authors: J
authors: Sun
authors: Yougong
authors: Y
authors: Su
authors: Yongwen
authors: Y
authors: Chen
authors: Jing
authors: J
authors: Yuan
authors: Yongyi
authors: Y
authors: Bi
authors: Qingyi
authors: Q
authors: Wei
authors: Tangchun
authors: T
authors: Wu
mesh_terms: D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000032:analysis*; D000328:Adult; D000395:Air Pollutants, Occupational / Q000633:toxicity; D016022:Case-Control Studies; D002681:China; D002853:Chromatography, Liquid; D003077:Coke / Q000633:toxicity*; D020552:Comet Assay; D018736:DNA Adducts / Q000097:blood*; D004249:DNA Damage; D019652:Extraction and Processing Industry; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry*; D008297:Male; D016273:Occupational Exposure; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity; D012307:Risk Factors; D013232:Steel
chemical_list: D000395:Air Pollutants, Occupational; D003077:Coke; D018736:DNA Adducts; D011084:Polycyclic Aromatic Hydrocarbons; D013232:Steel; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide