[Effect of Shensu Yin on the expression of toll-like receptors and the downstream signaling components on RAW 264.7 cells].
OBJECTIVE
To investigate the influences of Shensu Yin to RAW 264.7 on the expression of TLR3, TLR4 and the factors of the downstream in RAW 264. 7 cells.


METHODS
RAW 264.7 cell line was stimulated with Lipopolysaccharide and POLY I: C, respectively, and treated with the drug serum of Shensuyin simultaneously. 24 hours later, collected the supernatant and measured the inflammatory factors TNF-alpha and IFN-beta, extracted mRNA and measured the expression of TLR3, TLR4 and other correlated indexes of the downstream, analyzed and evaluated Shensu Yin's substance basis of pharmacodynamic actions.


RESULTS
Shensu Yin drug serum depressed the expression of TLR4, MyD88, TRAF-6, TRAM and TRIF mRNA, as a result, it decreased the amount of TNF-alpha and IFN-beta.


CONCLUSIONS
Depressing the expression of TLR3, MyD88, TRAM and TRIF mRNA may be the elementary basis of Shensu Yin to play heat-clearing and detoxicating effect.
Zhao
Bao-Sheng
BS
Tang Center for Herbal Medicine Research, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Li
Lan-Fang
LF
Ma
Yue-Ying
YY
Guo
Shu-Ying
SY
Li
Cang-Hai
CH
Huo
Hai-Ru
HR
Jiang
Ting-Liang
TL
D033942:Adaptor Proteins, Vesicular Transport / Q000235:genetics; D000818:Animals; D002460:Cell Line; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D016899:Interferon-beta / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics; D010946:Plants, Medicinal / Q000737:chemistry; D011070:Poly I-C / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D018123:Receptors, Interleukin / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects*; D051196:Toll-Like Receptor 3 / Q000235:genetics*; D051197:Toll-Like Receptor 4 / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D033942:Adaptor Proteins, Vesicular Transport; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D016899:Interferon-beta; D008070:Lipopolysaccharides; D053594:Myeloid Differentiation Factor 88; D011070:Poly I-C; D012333:RNA, Messenger; D018123:Receptors, Interleukin; D051196:Toll-Like Receptor 3; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha; C476286:Ticam1 protein, mouse; C476416:Ticam2 protein, mouse; C528926:shensuyin