Chondroitin-4-sulfate: a bioactive macromolecule to foster vascular healing around stent-grafts after endovascular aneurysm repair.
Deficient healing after endovascular aneurysm repair with a stent-graft is thought to be related to pro-apoptotic environment in abdominal aortic aneurysms and inertness of graft materials. We developed a bioactive coating containing chondroitin-4-sulfate and assessed its potential to improve cell adhesion, viability and resistance to apoptosis on PET surfaces. Coatings of collagen type I and CS were prepared and characterized by DMMB, FT-IR, DSC, SEM and contact angle goniometry. Preliminary cell culture experiments with vascular smooth muscle cells showed increased adhesion and viability in serum-free medium on CS-coated surfaces compared to control PET films.
Charbonneau
Cindy
C
Centre de Recherche, Centre Hospitalier de l'Université de Montréal, 1560 Sherbrooke Est, Montréal, QC, Canada H2L 4M1.
Gautrot
Julien E
JE
Hébert
Marie-Josée
MJ
Zhu
X X
XX
Lerouge
Sophie
S
D000818:Animals; D017544:Aortic Aneurysm, Abdominal / Q000601:surgery; D017209:Apoptosis; D001807:Blood Vessel Prosthesis; D001808:Blood Vessels; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002460:Cell Line; D002470:Cell Survival; D002809:Chondroitin Sulfates / Q000737:chemistry / Q000378:metabolism; D020099:Coated Materials, Biocompatible; D024042:Collagen Type I / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D008751:Methylene Blue / Q000031:analogs & derivatives / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D011093:Polyethylene Terephthalates / Q000737:chemistry; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared; D015607:Stents; D014945:Wound Healing
D020099:Coated Materials, Biocompatible; D024042:Collagen Type I; D011093:Polyethylene Terephthalates; C435946:dimethylmethylene blue; D002809:Chondroitin Sulfates; D008751:Methylene Blue