Genetic characterization of canine rotavirus isolated from a puppy in Korea and experimental reproduction of disease.
Canine rotavirus was isolated from feces of a Korean Jindo dog with mild diarrhea, and the isolate was genetically characterized. Rotaviral antigen was detected in the feces using a commercial rotavirus antigen detection kit and cytopathic effects were observed in a cell line inoculated with the feces. The virus isolate (GC/KS05) was identified as subtype G3P[3] using reverse transcription polymerase chain reaction (RT-PCR). The strain displayed 98% and 90% identity with the VP7 genes of a canine rotavirus isolate (RV52/96) from Italy and the simian rotavirus strain (RRV) respectively. However, the GC/KS05 isolate exhibited only 83% and 82% identity, respectively, with the G3 serotype canine strains, RV198/95 and K9. Phylogenetic analysis of the VP7 and VP4 genes of GC/KS05 strain led to the classification of VP7 in a different cluster than other canine rotavirus VP7 genes, and VP4 within the cluster of canine rotavirus VP4 genes. The Korean isolate was thus more closely related to the RV52/96 isolate than the other isolates for which sequence data is available. Detailed analysis of the VP7 region revealed 6 amino acid variations between the new isolate and RV52/96. After 5 passages in cell culture, the GC/KS05 strain remained pathogenic for young pups, in which inoculation resulted in diarrhea and virus shedding in the feces.
Kang
Bo Kyu
BK
Department of Veterinary Medicine Virology Lab, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
Song
Dae Sub
DS
Jung
Kwon Il
KI
Lee
Chul Seung
CS
Park
Sung Jun
SJ
Oh
Jin Sik
JS
An
Dong Jun
DJ
Yang
Jeong Sun
JS
Moon
Hyoung Joon
HJ
Lee
Sang Sun
SS
Yoon
Young Dhuk
YD
Park
Bong Kyun
BK
D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D007723:Korea; D008969:Molecular Sequence Data; D010802:Phylogeny; D012401:Rotavirus / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000662:veterinary* / Q000821:virology
D000956:Antigens, Viral; D036022:Capsid Proteins; C050779:VP7 protein, Rotavirus