Immunohistochemical expression of RARalpha, RARbeta, and Cx43 in breast tumor cell lines after treatment with lycopene and correlation with RT-QPCR.
Lycopene, the major carotenoid found in tomatoes, is a potent antioxidant associated with the prevention of degenerative diseases such as breast cancer. This effect could be due to the interaction between lycopene and retinoic acid receptors as well as the stimulation of gap junction communication and synthesis of connexin 43. The expression of the RARalpha, RARbeta, and Cx43 proteins was analyzed using immunohistochemistry in two breast cancer cell lines, MCF-7 and MDA-MB-231, and in a fibrocystic dystrophy cell line, MCF-10a, after a 48-hr exposure to 10 microM lycopene. A real-time quantitative PCR analysis was then performed to measure mRNA expression. RARalpha and Cx43 expression were increased at both mRNA and protein levels in two breast cell lines.
Chalabi
Nasséra
N
Département d'Oncogénétique, Centre Jean Perrin, Clermont-Ferrand Cedex 1, France.
Delort
Laetitia
L
Satih
Samir
S
Déchelotte
Pierre
P
Bignon
Yves-Jean
YJ
Bernard-Gallon
Dominique J
DJ
D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000473:pathology*; D002338:Carotenoids / Q000494:pharmacology*; D002460:Cell Line; D045744:Cell Line, Tumor; D018031:Connexin 43 / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D005348:Fibrocystic Breast Disease / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D000077276:Lycopene; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D018168:Receptors, Retinoic Acid / Q000096:biosynthesis* / Q000235:genetics; D000072482:Retinoic Acid Receptor alpha; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
D016588:Anticarcinogenic Agents; D018031:Connexin 43; D020033:Protein Isoforms; C000607071:RARA protein, human; D012333:RNA, Messenger; D018168:Receptors, Retinoic Acid; D000072482:Retinoic Acid Receptor alpha; C082925:retinoic acid receptor beta; D002338:Carotenoids; D000077276:Lycopene