title: Mesenchymal stem cells modified with angiopoietin-1 improve remodeling in a rat model of acute myocardial infarction.
abstract: We used human angiopoietin-1 (hAng1)-modified mesenchymal stem cells (MSCs) to treat acute myocardial infarction (AMI) in rats. The hAng1 gene was transfected into cultured rat MSCs using an adenoviral vector. Five million hAng-transfected MSCs (MSC(Ang1)) or green fluorescent protein transfected MSCs (MSC(GFP)) or PBS only (PBS group) were injected intramyocardially into the inbred Lewis rat hearts immediately after myocardial infarction. MSC(Ang1) survived in the infarcted myocardium, and expressed hAng1 at both mRNA and protein levels. The vascular density was higher in the MSC(Ang1) and MSC(GFP) groups than in the PBS group. The measurements of infarcted ventricular wall thickness, infarction area, and left ventricular diameter indicated that heart remodeling was inhibited and heart function was improved in both the MSC(Ang1) and MSC(GFP) groups. However, in contrast to the MSC(GFP) group, the MSC(Ang1) group showed enhanced angiogenesis and arteriogenesis (by 11-35%), infarction area was reduced by 30% and the left ventricular wall was 46% thicker (P<0.05). The results indicated that hAng1-modified MSCs improved heart function, followed by angiogenic effects in salvaging ischemic myocardium and reduced cardiac remodeling.
authors: Department of Cardiology, Peking University Third Hosptial, Beijing 100083, China.
authors: Lijie
authors: L
authors: Sun
authors: Ming
authors: M
authors: Cui
authors: Zuoyan
authors: Z
authors: Wang
authors: Xinheng
authors: X
authors: Feng
authors: Jieming
authors: J
authors: Mao
authors: Ping
authors: P
authors: Chen
authors: Ma
authors: M
authors: Kangtao
authors: Fengrong
authors: F
authors: Chen
authors: Chunyan
authors: C
authors: Zhou
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D042683:Angiopoietin-1 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D001158:Arteries / Q000254:growth & development; D015153:Blotting, Western; D004195:Disease Models, Animal; D004452:Echocardiography; D015870:Gene Expression; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006321:Heart / Q000502:physiology; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D009203:Myocardial Infarction / Q000503:physiopathology / Q000628:therapy*; D009206:Myocardium / Q000378:metabolism; D018919:Neovascularization, Physiologic; D051381:Rats; D011917:Rats, Inbred Lew; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D033581:Stem Cell Transplantation / Q000379:methods*; D014162:Transfection
chemical_list: D042683:Angiopoietin-1; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins