Differential protein expression in pancreatic islets after treatment with an imidazoline compound.
The effects of an imidazoline compound (BL11282) on protein expression in rat pancreatic islets were investigated with a proteomic approach. The compound increases insulin release selectively at high glucose concentrations and is therefore of interest in type 2 diabetes. Whole cell extracts from isolated drug-treated and native pancreatic rat islets were compared after separation by 2-D gel electrophoresis. Differentially expressed proteins were identified by mass spectrometry; 15 proteins were selectively up-regulated and 7 selectively down-regulated in drug-treated islets. Of special interest among the differentially expressed proteins are those involved in protein folding (Hsp60, protein disulfide isomerase, calreticulin), Ca(2+) binding (calgizzarin, calcyclin and annexin I) and metabolism or signalling (pyruvate kinase, alpha enolase and protein kinase C inhibitor 1).
Jägerbrink
T
T
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
Lexander
H
H
Palmberg
C
C
Shafqat
J
J
Sharoyko
V
V
Berggren
P-O
PO
Efendic
S
S
Zaitsev
S
S
Jörnvall
H
H
D000818:Animals; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D007093:Imidazoles / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar
C423844:BL 11282; D007093:Imidazoles; D020543:Proteome