title: [Therapeutic effect of autologous cytokine-induced killer cells on patients with liver cirrhosis caused by HBV infection].
abstract: OBJECTIVE
  To observe the therapeutic effect of autologous cytokine-induced killer cells (CIK) on HBV DNA positive patients with liver cirrhosis.
  
  
  METHODS
  HBV DNA positive 33 patients with cirrhosis were treated with CIK. Before and after cultured in vitro and post-treatment, CD3+, CD3+CD4+, CD3+CD8+, CD3+CD56+ cells, mDC and pDC were detected by flow cytometry. The indexes of virus and liver function were compared between pre- and post-treatment.
  
  
  RESULTS
  CD3+, CD3+CD8+ cells and CD3+CD56+ cells were higher after cultured in vitro and after transfused back than those before culture (91.5 +/- 10.3, 74.4 +/- 9.9 vs. 67.9 +/- 12.8; 60.9 +/- 15.5, 37.3 +/- 15.1 vs. 27.9 +/- 10.9; 18.4 +/- 11.7, 14.5 +/- 7.5 vs. 10.6 +/- 7.1). The percentages of mDC and pDC also increased after-treatment vs. pre-treatment (0.54 +/- 0.18 vs. 0.70 +/- 0.29; 0.26 +/- 0.13 vs. 0.41 +/- 0.25). HBV DNA became undetectable in 12 patients and decrease exceeded 100 times in 4 patients after treatment. HBeAg became undetectable in 10 of 14 patients who were HBeAg positive pretreatment patients, among them 2 patients had HBeAb sero conversion. The liver function was improved after treatment. All patients tolerated the treatment.
  
  
  CONCLUSIONS
  CIK treatment can increase immune effector cells and has some antiviral effect and is safe.
authors: The No. 302 Hospital of the People's Liberation Army, Beijing 100039, China.
authors: Hai-bin
authors: HB
authors: Su
authors: Han-wei
authors: HW
authors: Li
authors: Hong-lan
authors: HL
authors: Zhao
authors: Ming
authors: M
authors: Shi
authors: Bing
authors: B
authors: Zhang
authors: Zi-rong
authors: ZR
authors: Tang
authors: Zhou-yun
authors: ZY
authors: Lei
authors: Hui-fen
authors: HF
authors: Wang
authors: Fu-sheng
authors: FS
authors: Wang
mesh_terms: D019264:Adoptive Transfer / Q000009:adverse effects / Q000379:methods*; D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D055612:Cytokine-Induced Killer Cells / Q000166:cytology / Q000276:immunology / Q000637:transplantation*; D005221:Fatigue / Q000209:etiology; D005260:Female; D006261:Headache / Q000209:etiology; D006509:Hepatitis B / Q000150:complications* / Q000821:virology; D006801:Humans; D008103:Liver Cirrhosis / Q000209:etiology / Q000276:immunology / Q000628:therapy*; D008297:Male; D008875:Middle Aged; D014182:Transplantation, Autologous; D016896:Treatment Outcome