Different patterns in the induction of metallothionein mRNA synthesis among isoforms after acute ethanol administration.
The induction of metallothionein (MT) isoform synthesis was investigated in mouse cerebral cortex 18 h after oral ethanol administration. The expression of MT-I isoform mRNA increased in a dose-dependent manner after ethanol loading at doses between 2 g/kg (ethanol/body weight) and 8 g/kg. Lipid peroxide formation, measured as the amount of malondialdehyde- reactive substances, remained at the control level after all of the administered ethanol doses. The expression of MT-III isoform mRNA remained at the control level up until an ethanol loading dose of 4 g/kg and then finally increased to a significant level at a dose of 8 g/kg, which is almost the LD50 for oral ethanol in mice. The different patterns of MT synthesis induction among MT isoforms suggests that the MT-I isoform, which is ubiquitous in mammalian tissues, plays a significant role as an antioxidant. On the other hand, the MT-III isoform, which has a limited tissue distribution, especially in the central nervous system, seems to be implicated in tissue repair and/or protection against critical tissue injury.
Ono
Shin-Ichi
S
Laboratory of Clinical Pharmacy, College of Pharmacy, Nihon University, 7-7-1 Narashino-Dai, Funabashi, Chiba, Japan.
Ishizaki
Yuriko
Y
Tokuda
Ei-Ichi
E
Tabata
Kei-Ichi
K
Asami
Satoru
S
Suzuki
Takashi
T
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D000431:Ethanol / Q000008:administration & dosage* / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008668:Metallothionein / Q000096:biosynthesis* / Q000235:genetics*; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D013997:Time Factors
D020033:Protein Isoforms; D012333:RNA, Messenger; D000431:Ethanol; D008668:Metallothionein; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases