Status epilepticus triggers caspase-3 activation and necrosis in the immature rat brain.
The mode and mechanism of neuronal death induced by status epilepticus (SE) in the immature brain have not been fully characterized. In this study, we analyzed the contribution of neuronal necrosis and caspase-3 activation to CA1 damage following lithium-pilocarpine SE in P14 rat pups. By electron microscopy, many CA1 neurons displayed evidence of early necrosis 6 hours following SE, and the full ultrastructural features of necrosis at 24-72 hours. Caspase-3 was activated in injured (acidophilic) neurons 24 hours following SE, raising the possibility that they died by caspase-dependent "programmed" necrosis.
Niquet
Jerome
J
Department of Neurology, David Geffen School of Medicine at UCLA, Los Angeles, California, USA. jniquet@ucla.edu
Auvin
Stéphane
S
Archie
Mark
M
Seo
Dae-Won
DW
Allen
Suni
S
Sankar
Raman
R
Wasterlain
Claude G
CG
D000818:Animals; D000831:Animals, Newborn; D001343:Autophagy; D001921:Brain / Q000201:enzymology* / Q000473:pathology* / Q000648:ultrastructure; D053148:Caspase 3 / Q000378:metabolism*; D016923:Cell Death; D004195:Disease Models, Animal; D016291:Dizocilpine Maleate / Q000494:pharmacology; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005260:Female; D018021:Lithium Chloride / Q000494:pharmacology; D008297:Male; D008854:Microscopy, Electron; D009336:Necrosis / Q000473:pathology; D009474:Neurons / Q000473:pathology; D010862:Pilocarpine / Q000494:pharmacology; D017966:Pyramidal Cells / Q000201:enzymology / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D013226:Status Epilepticus / Q000139:chemically induced / Q000201:enzymology* / Q000473:pathology*
D018691:Excitatory Amino Acid Antagonists; D010862:Pilocarpine; D016291:Dizocilpine Maleate; C505410:Casp3 protein, rat; D053148:Caspase 3; D018021:Lithium Chloride