Preparation of multiwalled carbon nanotubes-platinum-Nafion-glucose oxidase nanobiocomposite and its application to glucose biosensor.
Platinum (Pt) nanoparticles were electrodeposited within multiwalled carbon nanotubes-Nafion-glucose oxidase (MWNTs-Nafion-GOx) nanobiocomposite by a potentiostatic method. The morphology and nature of the resulting MWNTs-Pt-Nafion-GOx nanobiocomposite were characterized by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), and energy dispersive X-ray spectroscopy (EDS). The electrocatalytic properties of the MWNTs-Pt-Nafion-GOx nanobiocomposite film modified glassy carbon electrode were characterized by cyclic voltammetry and amperometry in the presence of hydrogen peroxide. The glucose biosensor sensitivity was strongly influenced by the deposits of Pt nanoparticles and amount of GOx concentration within the MWNTs-Pt-Nafion-GOx nanobiocomposite film. The optimized glucose biosensor displayed a sensitivity of 640 nA mM(-1), a linear range of up to 4 mM, a detection limit of 4 microM, and a response time of less than 4 s at an operating potential of +500 mV versus Ag/AgCl (3 M KCl).
Tsai
Yu-Chen
YC
Department of Chemical Engineering, National Chung Hsing University, Taichung 402, Taiwan.
Chien
Hui-Yu
HY
D015374:Biosensing Techniques / Q000295:instrumentation*; D004563:Electrochemistry / Q000379:methods; D005465:Fluorocarbon Polymers / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008956:Models, Chemical; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry; D013052:Spectrometry, X-Ray Emission
D005465:Fluorocarbon Polymers; D037742:Nanotubes, Carbon; C040402:perfluorosulfonic acid; D010984:Platinum; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase