title: [Function of the putative Na+/H+ antiporter gene PeNhaD1 from salt-resistant Populus euphratica Oliv].
abstract: Yeast complementation experiments were carried out to define the possible function of PeNhaD1, a Na(+)/H(+) antiporter gene from Populus euphratica Oliv., a salt resistant tree. PeNhaD1 was introduced to the Saccharomyces cerevisiae mutant strain ANT3 (Deltaena1-4::HIS3 Deltanha1::LEU2), which lacks the plasma membrane Na(+)/H(+) antiporter gene ScNHA1 or GX1 (Deltanhx1::TRP1), which lacks tonoplast Na(+)/H(+) antiporter gene ScNHX1. Our results showed that PeNhaD1 rescued the normal growth of ANT3 in the presence of high salt (80 mmol/L NaCl on solid medium or 400 mmol/L in liquid medium, pH 6.0), but not that of GX1, suggesting that PeNhaD1 may play a role in salt tolerance of Populus euphratica by maintaining the capacity for salt exclusion under saline condition.
authors: College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
authors: Ping-Ping
authors: PP
authors: Lv
authors: Jun
authors: J
authors: Hu
authors: Shao-Liang
authors: SL
authors: Chen
authors: Xin
authors: X
authors: Shen
authors: Wei-Bo
authors: WB
authors: Yin
authors: Yu-Hong
authors: YH
authors: Chen
authors: Yong-Ru
authors: YR
authors: Sun
authors: Zan-Min
authors: ZM
authors: Hu
mesh_terms: D015870:Gene Expression / Q000187:drug effects; D005816:Genetic Complementation Test; D009154:Mutation; D010940:Plant Proteins / Q000235:genetics / Q000502:physiology*; D032107:Populus / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D012965:Sodium Chloride / Q000494:pharmacology*; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics / Q000502:physiology*
chemical_list: D010940:Plant Proteins; D017923:Sodium-Hydrogen Exchangers; D012965:Sodium Chloride