[Prolonged hypoxia induces GST-pi expression and drug resistance in lung adenocarcinoma cell line SPCA1].
OBJECTIVE
To investigate the association of hypoxia with GST-pi expression in lung adenocarcinoma cells SPCA1 and the drug resistance in hypoxic condition.


METHODS
RNA and protein in SPCA1 cells treated with hypoxia (0.5% O(2)) for 16 h were isolated. HIF-1alpha and GST-pi mRNA expression was detected by real time RT-PCR, and HIF-1alpha protein was detected by Western blotting analysis. GST-pi protein was finally analyzed on a FACScan flow cytometer using Cellquest software. Sensitivity of Doxorubicin and Mitomycin in hypoxic SPCA1 cells was detected using cell clonogenic assay.


RESULTS
HIF-1alpha expression was up-regulated on mRNA and protein levels in hypoxic SPCA1 cells, so as was GST-pi expression. Doxorubicin resistance was increased, but hypoxia had no significant influence on Mitomycin resistance. GST-pi expression had no significant change after HIF-1alpha was down-regulated by RNA interfering.


CONCLUSIONS
Though prolonged hypoxia can induce GST-pi expression and increase drug resistance such as to Doxorubicin, HIF-1alpha expression has no correlation with GST-pi expression.
Liu
Yong-lei
YL
Center of Cancer Research, Shandong Tumor Hospital and Institute, Jinan 250117, China. liu-yonglei@163.com
Song
Xian-rang
XR
Wang
Xing-wu
XW
Wei
Ling
L
Li
Da-peng
DP
D000230:Adenocarcinoma / Q000378:metabolism*; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm; D015251:Epirubicin / Q000494:pharmacology; D051549:Glutathione S-Transferase pi / Q000378:metabolism*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism; D008175:Lung Neoplasms / Q000378:metabolism*; D016685:Mitomycin / Q000494:pharmacology; D010957:Plasmids; D014162:Transfection
D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D015251:Epirubicin; D016685:Mitomycin; D051549:Glutathione S-Transferase pi