Protein immobilization strategies for protein biochips.
In the past few years, protein biochips have emerged as promising proteomic and diagnostic tools for obtaining information about protein functions and interactions. Important technological innovations have been made. However, considerable development is still required, especially regarding protein immobilization, in order to fully realize the potential of protein biochips. In fact, protein immobilization is the key to the success of microarray technology. Proteins need to be immobilized onto surfaces with high density in order to allow the usage of small amount of sample solution. Nonspecific protein adsorption needs to be avoided or at least minimized in order to improve detection performances. Moreover, full retention of protein conformation and activity is a challenging task to be accomplished. Although a large number of review papers on protein biochips have been published in recent years, few have focused on protein immobilization technology. In this review, current protein immobilization strategies, including physical, covalent, and bioaffinity immobilization for the fabrication of protein biochips, are described. Particular consideration has been given to oriented immobilization, also referred to as site-specific immobilization, which is believed will improve homogeneous surface covering and accessibility of the active site.
Rusmini
Federica
F
Department of Polymer Chemistry and Biomaterials (PBM), Institute for Biomedical Technology (BMTI), Faculty of Science and Technology, University of Twente, Enschede, 7500 AE, The Netherlands.
Zhong
Zhiyuan
Z
Feijen
Jan
J
D000818:Animals; D001710:Biotin / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry; D006801:Humans; D046911:Macromolecular Substances; D008956:Models, Chemical; D008968:Molecular Conformation; D010455:Peptides / Q000737:chemistry; D040081:Protein Array Analysis; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D040901:Proteomics / Q000295:instrumentation; D013499:Surface Properties
D004800:Enzymes, Immobilized; D046911:Macromolecular Substances; D010455:Peptides; D011506:Proteins; D001710:Biotin