title: The outer plate in vertebrate kinetochores is a flexible network with multiple microtubule interactions.
abstract: Intricate interactions between kinetochores and microtubules are essential for the proper distribution of chromosomes during mitosis. A crucial long-standing question is how vertebrate kinetochores generate chromosome motion while maintaining attachments to the dynamic plus ends of the multiple kinetochore MTs (kMTs) in a kinetochore fibre. Here, we demonstrate that individual kMTs in PtK(1) cells are attached to the kinetochore outer plate by several fibres that either embed the microtubule plus-end tips in a radial mesh, or extend out from the outer plate to bind microtubule walls. The extended fibres also interact with the walls of nearby microtubules that are not part of the kinetochore fibre. These structural data, in combination with other recent reports, support a network model of kMT attachment wherein the fibrous network in the unbound outer plate, including the Hec1-Ndc80 complex, dissociates and rearranges to form kMT attachments.
authors: Department of Biomedical Sciences, School of Public Health, State University of New York at Albany, Albany, NY 12201, USA.
authors: Yimin
authors: Y
authors: Dong
authors: Kristin J
authors: KJ
authors: Vanden Beldt
authors: Xing
authors: X
authors: Meng
authors: Alexey
authors: A
authors: Khodjakov
authors: Bruce F
authors: BF
authors: McEwen
mesh_terms: D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D004847:Epithelial Cells / Q000378:metabolism* / Q000648:ultrastructure; D018386:Kinetochores / Q000378:metabolism* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism* / Q000648:ultrastructure; D008938:Mitosis / Q000502:physiology*; D008958:Models, Molecular; D009687:Nuclear Proteins / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D014057:Tomography, X-Ray Computed; D014714:Vertebrates
chemical_list: D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins