Antinucleosome antibody-modified liposomes and lipid-core micelles for tumor-targeted delivery of therapeutic and diagnostic agents.
Liposomes and lipid-core micelles prepared of polyethylene glycol-phosphatidylethanolamine (PEG-PE) conjugates have been modified with nucleosome-specific monoclonal antinuclear autoantibody (ANA) 2C5 (mAb 2C5) specifically recognizing a broad variety of cancer cells through the cancer cell surface-bound nucleosomes. mAb 2C5 preserves its specific properties upon the binding with the lipid-based pharmaceutical nanocarriers, and 2C5-modified immunoliposomes and immunomicelles demonstrate an enhanced binding with tumor cells both in vitro and in vivo. We have investigated the delivery of therapeutic and diagnostic agents with such tumor-targeted immunoliposomes and immunomicelles to various tumors in vivo and in vitro. Both lipid-based nanocarriers provided enhanced tumor delivery of imaging agents ((111)In) and antitumor drugs (doxorubicin and photodynamic therapy agents) to tumor cells under different experimental settings. Pharmaceutical lipid-based nanoparticular carriers modified with mAb 2C5 could represent universal systems for tumor-specific delivery of various soluble and insoluble pharmaceuticals.
Elbayoumi
Tamer A
TA
Department of Pharmaceutical Sciences, Northeastern University, Boston, MA 02115, USA.
Pabba
Santosh
S
Roby
Aruna
A
Torchilin
Vladimir P
VP
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage; D000974:Antibodies, Antinuclear / Q000008:administration & dosage*; D000911:Antibodies, Monoclonal / Q000008:administration & dosage*; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000008:administration & dosage; D004337:Drug Carriers; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008081:Liposomes; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008823:Micelles; D009707:Nucleosomes / Q000276:immunology; D004369:Pentetic Acid; D016719:Radioimmunodetection
D000903:Antibiotics, Antineoplastic; D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D004337:Drug Carriers; D008081:Liposomes; D008823:Micelles; D009707:Nucleosomes; D004369:Pentetic Acid; D004317:Doxorubicin