Cultured epithelial cells response to phototherapy with low intensity laser.
OBJECTIVE
Little is known about the intracellular response of epithelial cells to phototherapy. The aim of this in vitro study was to analyze the effect of phototherapy with low-energy lasers with different wavelengths and powers on cultured epithelial cell growth under different nutritional conditions.


METHODS
Epithelial cell cultures (Vero cell line) grown in nutritional deficit in culture medium supplemented with 2% fetal bovine serum (FBS) were irradiated with low-energy laser from one to three times with a GaAlAs laser (660 nm) and InGaAlP (780 nm), 40 and 70 mW, respectively, with 3 or 5 J/cm2. Cell growth was indirectly assessed by measuring the cell mitochondrial activity.


RESULTS
Nonirradiated cell cultures grown in nutritional regular medium supplemented with 10% FBS produced higher cell growth than all cultures grown in nutritional deficit irradiated or not. The overall cell growth of cultures grown under nutritionally deficit conditions was significantly improved especially when irradiated with 780 nm for three times.


CONCLUSIONS
Phototherapy with the laser parameters tested increases epithelial cell growth rate for cells stressed by growth under nutritionally deficient states. This cell growth improvement is directly proportional to the number of irradiations; however, was not enough to reach the full cell growth potential rate of Vero epithelial cell line observed when growing under nutritional regular condition.
Eduardo
Fernanda P
FP
Department of Oral Diagnosis, School of Dentistry, University of São Paulo, São Paulo, Brazil.
Mehnert
Dolores U
DU
Monezi
Telma A
TA
Zezell
Denise M
DM
Schubert
Mark M
MM
Eduardo
Carlos P
CP
Marques
Márcia M
MM
D000818:Animals; D002417:Cattle; D049109:Cell Proliferation / Q000528:radiation effects; D002478:Cells, Cultured; D003470:Culture Media; D004847:Epithelial Cells / Q000528:radiation effects*; D005312:Fetal Blood; D000882:Haplorhini; D008928:Mitochondria / Q000528:radiation effects; D010789:Phototherapy / Q000379:methods*
D003470:Culture Media