HPLC-photodiode array detection analysis of curcuminoids in Curcuma species indigenous to Indonesia.
An optimized HPLC method with photodiode array detection was developed and applied to analyse the curcuminoids curcumin, demethoxycurcumin, and bis-demethoxycurcumin in rhizomes of Curcuma mangga Val &. v. Zijp, C. heyneana Val. & v. Zijp, C. aeruginosa Roxb. and C. soloensis Val. (Zingiberaceae), indigenous to Indonesia. The method was validated with an isocratic system, a short run time of 10 min and a baseline separation. The curcuminoid content was 0.18-0.47% for C. mangga, 0.98-3.21% for C. heyneana, 0.02-0.03% for C. aeruginosa and 0.40% for C. soloensis.
Bos
Rein
R
Department of Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1,9713 AV Groningen, The Netherlands. R.Bos@rug.nl
Windono
Tri
T
Woerdenbag
Herman J
HJ
Boersma
Ykelien L
YL
Koulman
Albert
A
Kayser
Oliver
O
D002851:Chromatography, High Pressure Liquid; D030024:Curcuma / Q000737:chemistry*; D003474:Curcumin / Q000031:analogs & derivatives* / Q000032:analysis*; D007202:Indicators and Reagents; D007214:Indonesia; D018517:Plant Roots / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D013045:Species Specificity
D007202:Indicators and Reagents; D003474:Curcumin