title: [Determination of bufothionine in skin of Bufo bufo gargarizans and Huachansu injection].
abstract: OBJECTIVE
  To establish a HPLC method for bufothionine in the skin of Bufo bufo gargarizans and Huachansu injection.
  
  
  METHODS
  The samples were separated using a Lichrosob C18 column with CH3CN-H2O (10:90) as mobile phase. Flow rate was at 1.0 mL x min (-1) and the detection wavelength was at 225 nm.
  
  
  RESULTS
  The calibration curve of bufothionine was linear over the range of 0.0772-0.4632 microg and the average recovery was 99. 2%. The contents of bufothionine were fluctuated from 36.4-641.8 microg x g(-1) in the skin of Bufo bufo gargarizans and 22.47-33.16 microg x mL(-1) in Huachansu injection, respectively.
  
  
  CONCLUSIONS
  The contents of bufothionine were greatly different between cultured and wild species. The method was suitable for the quality control of the skin of Bufo bufo gargarizans and its preparation.
authors: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors: Li-ping
authors: LP
authors: Dai
authors: Zhi-min
authors: ZM
authors: Wang
authors: Hui-min
authors: HM
authors: Gao
authors: Xu
authors: X
authors: Jiang
authors: Guang-zhi
authors: GZ
authors: Ding
mesh_terms: D000818:Animals; D002023:Bufo bufo; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D007267:Injections; D008419:Materia Medica / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D010640:Phenothiazines / Q000032:analysis*; D011786:Quality Control; D015203:Reproducibility of Results; D012867:Skin / Q000737:chemistry*
chemical_list: D008419:Materia Medica; D010640:Phenothiazines; C009469:thionine