title: Phenotypes of IRS-2 deficient mice produced by reproductive technology are stable.
abstract: We studied the impact of "IVF - ET" on the glucose tolerance test (GTT), insulin tolerance test (ITT) and adiponectin to investigate differences in the phenotypes of B6J- Irs2(-/-) mice. The B6J-Irs2(-/-) mice (KO-Nat group) were prepared by natural mating. Other mice were produced by IVF-ET used ICR strain recipients and surrogate mothers (KO-IVF group). Measurement of body weight, GTT, ITT and blood sampling were performed at the ages of 6, 14 and 24 weeks after birth. Body weights, impaired glucose tolerance, insulin resistance and plasma adiponectin concentrations did not differ for each gender between the KO-IVF and KO-Nat groups. Therefore, we concluded that phenotypes of Irs2(-/-) mice produced by reproductive technology are stable.
authors: Central Institute for Experimental Animals, Kawasaki-Shi, Kanagawa, Japan.
authors: Haruo
authors: H
authors: Hashimoto
authors: Toshiro
authors: T
authors: Arai
authors: Yasuyuki
authors: Y
authors: Ohnishi
authors: Tomoo
authors: T
authors: Eto
authors: Mamoru
authors: M
authors: Ito
authors: Kyoji
authors: K
authors: Hioki
authors: Ryo
authors: R
authors: Suzuki
authors: Toshimasa
authors: T
authors: Yamauchi
authors: Mitsuru
authors: M
authors: Ohsugi
authors: Muneo
authors: M
authors: Saito
authors: Yoshito
authors: Y
authors: Ueyama
authors: Kazuyuki
authors: K
authors: Tobe
authors: Takashi
authors: T
authors: Kadowaki
authors: Norikazu
authors: N
authors: Tamaoki
authors: Kinori
authors: K
authors: Kosaka
mesh_terms: D052242:Adiponectin / Q000097:blood; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight / Q000235:genetics; D003307:Copulation / Q000502:physiology*; D003921:Diabetes Mellitus, Experimental / Q000235:genetics / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro; D020868:Gene Silencing; D005951:Glucose Tolerance Test; D007178:Inbreeding; D055504:Insulin Receptor Substrate Proteins; D007333:Insulin Resistance / Q000235:genetics; D047908:Intracellular Signaling Peptides and Proteins / Q000097:blood / Q000172:deficiency / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010641:Phenotype; D010750:Phosphoproteins / Q000097:blood / Q000172:deficiency / Q000235:genetics*; D011972:Receptor, Insulin / Q000097:blood / Q000172:deficiency / Q000235:genetics*; D013047:Specific Pathogen-Free Organisms
chemical_list: D052242:Adiponectin; D001786:Blood Glucose; D055504:Insulin Receptor Substrate Proteins; D047908:Intracellular Signaling Peptides and Proteins; C526298:Irs2 protein, mouse; D010750:Phosphoproteins; D011972:Receptor, Insulin