title: MDR1 gene polymorphisms and risk of recurrence in patients with hepatocellular carcinoma after liver transplantation.
abstract: OBJECTIVE
  Recurrence of hepatocellular carcinoma (HCC) after liver transplantation (LT) remains a major cause of post-LT death. However, currently there is still lacking the markers to reliably predict recurrence. This study was undertaken to evaluate the association between three polymorphisms (C1236T, G2677A/T, C3435T) of Multidrug resistance 1 (MDR1) gene and the risk of recurrence after LT.
  
  
  METHODS
  Genomic DNA of 99 HCC patients undergoing LT was extracted from peripheral blood lymphocytes and genotyping was performed using polymerase chain reaction-restriction fragment length polymorphism assay. Cox proportional hazard model was used to estimate the hazard ratios associated with polymorphisms.
  
  
  RESULTS
  During a mean follow-up of 14.9 months, 49 patients experienced recurrence. The association between recurrence-free and 2677A carrier (carrying at least one variant A allele) was significant (P = 0.019). However, no significant association was observed in other polymorphisms. Patients with 2677A carrier conferred a 63% reduction in recurrence risk compared with 2677A non-carrier (odds ratio: 0.374; 95% confidence interval: 0.177-0.788; P = 0.010). The median recurrence-free survival for 2677A carrier group was significantly longer than that for 2677A non-carrier group (44.2 vs. 10.5 months, P = 0.015).
  
  
  CONCLUSIONS
  The polymorphism of MDR1 gene may be a valuable molecular marker for HCC recurrence after LT.
authors: Key Lab of Combined Multi-Organ Transplantation, Ministry of Public Health, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, People's Republic of China.
authors: Liming
authors: L
authors: Wu
authors: Xiaobo
authors: X
authors: Xu
authors: Juwei
authors: J
authors: Shen
authors: Haiyang
authors: H
authors: Xie
authors: Songfeng
authors: S
authors: Yu
authors: Tingbo
authors: T
authors: Liang
authors: Weilin
authors: W
authors: Wang
authors: Yan
authors: Y
authors: Shen
authors: Min
authors: M
authors: Zhang
authors: Shusen
authors: S
authors: Zheng
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics*; D000293:Adolescent; D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000601:surgery; D005260:Female; D019450:Genes, MDR; D005838:Genotype; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000601:surgery; D016031:Liver Transplantation; D008297:Male; D008875:Middle Aged; D008967:Molecular Biology; D015999:Multivariate Analysis; D009364:Neoplasm Recurrence, Local / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D012306:Risk
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1