title: Micronucleus rate of buccal mucosal epithelial cells in relation to oral hygiene and dental factors.
abstract: Carcinogenesis of squamous cell carcinomas in the upper aero-digestive tract (UADT) is a multi-stage process. Since 1937, micronuclei (MN) have been considered a marker for genome damage in the initiation stage. By help of the micronucleus test, carcinogenic exposure can be proven in the mucosa area of the UADT. The hypothesis to be tested was that individual oral hygiene and the dental status, respectively - just like alcohol and tobacco abuse - are associated with the micronucleus rate in cytological preparations of the buccal mucosa. In a prospective clinical observation study, we determined in 100 probands the micronucleus frequency per 1000 mucosa epithelial cells. Study participants with a high number of missing teeth (M/T index, p=0.037), a below-average papillary bleeding index (PBI, p=0.032) and periodontal status, respectively (PSI, p=0.042) possessed a higher micronucleus number in comparison with restored dental conditions. Probands with composite restorations displayed a higher MN rate (p=0.006) compared to those with amalgam. However, we could not detect any significant relation with the prosthetic status (p> or =0.075). An adjustment was made according to alcohol and tobacco. We therefore conclude that subgingival plaque and synthetic dental materials in addition to chronic alcohol and tobacco consumption might have genotoxic relevance in the oral cavity.
authors: ENT - Department of Saarland University, Homburg/Saar, Germany.
authors: Marc
authors: M
authors: Bloching
authors: Waldemar
authors: W
authors: Reich
authors: Johannes
authors: J
authors: Schubert
authors: Tamara
authors: T
authors: Grummt
authors: Annett
authors: A
authors: Sandner
mesh_terms: D000328:Adult; D000367:Age Factors; D000428:Alcohol Drinking; D003188:Composite Resins / Q000009:adverse effects; D004245:DMF Index; D003773:Dental Plaque; D017266:Dental Prosthesis; D004847:Epithelial Cells / Q000648:ultrastructure*; D005260:Female; D006801:Humans; D007779:Lactobacillus acidophilus; D008297:Male; D048629:Micronuclei, Chromosome-Defective; D015162:Micronucleus Tests; D008875:Middle Aged; D009061:Mouth Mucosa; D009910:Oral Hygiene; D010510:Periodontal Diseases / Q000473:pathology; D012307:Risk Factors; D012737:Sex Factors; D012907:Smoking; D013290:Streptococcal Infections / Q000473:pathology; D013295:Streptococcus mutans
chemical_list: D003188:Composite Resins