Antigenic characterization of phocine distemper virus causing mass mortality in 2002 and its relationship to other morbilliviruses.
The antigenic relationship between the phocine distemper virus (PDV) strain causing the epidemic in 2002 and the PDV strain of 1988, canine distemper virus from two dogs and one marten, and one measles virus strain was investigated in vivo and in vitro using monospecific polyclonal and monoclonal antibodies directed against five different proteins of canine or phocine distemper virus (N, P, M, F, H). Epitopic mapping revealed no difference between the PDV strains causing the epidemics in 1988 or 2002. However, the use of these antibodies allowed discrimination between different morbilliviruses including a vaccine strain of canine distemper virus. The major differences among the investigated morbilliviruses were found in the H protein.
Wohlsein
P
P
Department of Pathology, University of Veterinary Medicine, Hannover, Germany. peter.wohlsein@tiho-hannover.de
Müller
G
G
Haas
L
L
Siebert
U
U
Harder
T C
TC
Baumgärtner
W
W
D000818:Animals; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000276:immunology* / Q000378:metabolism; D002522:Chlorocebus aethiops; D004196:Disease Outbreaks; D004216:Distemper / Q000453:epidemiology* / Q000401:mortality / Q000821:virology; D004217:Distemper Virus, Canine / Q000145:classification / Q000276:immunology; D018104:Distemper Virus, Phocine / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity; D004285:Dogs; D018604:Epitope Mapping; D018103:Morbillivirus / Q000145:classification / Q000276:immunology*; D018185:Morbillivirus Infections; D045969:Mustelidae / Q000821:virology; D046048:Seals, Earless / Q000821:virology; D014709:Vero Cells; D014764:Viral Proteins / Q000276:immunology* / Q000378:metabolism
D000956:Antigens, Viral; D014764:Viral Proteins