title: Mitochondrial carrier family: repertoire and peculiarities of the cellular slime mould Dictyostelium discoideum.
abstract: Proteins of the mitochondrial carrier family (MCF) mediate the transport of a large range of compounds, including metabolites and cofactors. They are localized mainly in the inner mitochondrial membrane, except for a few members found in the membranes of peroxisomes. Similarity searches among Dictyostelium discoideum protein sequences identified a total of 31 MCF members. All these are membrane proteins that possess three characteristic repeats of a domain of approximately 100 residues. Among them, three proteins have supplementary structural domains consisting of Ca(2+)-binding motifs made up of 2 or 4 EF-hand units localized on the N-terminal end, facing the mitochondrial intermembrane space. The nature of transported substrates is proposed on the basis of sequence comparison with orthologs characterized biochemically in other organisms, of phylogenetic analysis, and of the conservation of discriminating amino acid residues belonging to the substrate binding sites. Carriers have been grouped in subclasses based on their specificity for the transport of nucleotides, amino acids or keto acids. Furthermore, we have identified an iron carrier of the mitoferrin type, an inorganic phosphate carrier, and three carriers with similarity to uncoupler proteins. This study provides a focus for mitochondrial carrier analysis in Dictyostelium discoideum.
authors: Laboratoire de Biochimie et Biophysique des Systèmes Intégrés, iRTSV/BBSI, UMR 5092 CNRS-CEA-UJF, CEA-Grenoble, 17 rue des Martyrs, 38054 Grenoble cedex 9, France.
authors: Michel
authors: M
authors: Satre
authors: Sara
authors: S
authors: Mattei
authors: Laurence
authors: L
authors: Aubry
authors: Pascale
authors: P
authors: Gaudet
authors: Ludovic
authors: L
authors: Pelosi
authors: Gérard
authors: G
authors: Brandolin
authors: Gérard
authors: G
authors: Klein
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites / Q000235:genetics; D004023:Dictyostelium / Q000235:genetics*; D000226:Mitochondrial ADP, ATP Translocases / Q000737:chemistry / Q000235:genetics; D033681:Mitochondrial Membrane Transport Proteins / Q000737:chemistry / Q000235:genetics*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid
chemical_list: D033681:Mitochondrial Membrane Transport Proteins; D000226:Mitochondrial ADP, ATP Translocases