Modeling the proteome of a Marek's disease transformed cell line: a natural animal model for CD30 overexpressing lymphomas.
Marek's disease (MD) in the chicken, caused by the highly infectious MD alpha-herpesvirus (MDV), is both commercially important and a unique, naturally occurring model for human T-cell lymphomas overexpressing the Hodgkin's disease antigen, CD30. Here, we used proteomics as a basis for modeling the molecular functions and biological processes involved in MDV-induced lymphomagenesis. Proteins were extracted from an MDV-transformed cell line and were then identified using 2-D LC-ESI-MS/MS. From the resulting 3870 cellular and 21 MDV proteins we confirm the existence of 3150 "predicted" and 12 "hypothetical" chicken proteins. The UA-01 proteome is proliferative, differentiated, angiogenic, pro-metastatic and pro-immune-escape but anti-programmed cell death, -anergy, -quiescence and -senescence and is consistent with a cancer phenotype. In particular, the pro-metastatic integrin signaling pathway and the ERK/MAPK signaling pathways were the two predominant signaling pathways represented. The cytokines, cytokine receptors, and their related proteins suggest that UA-01 has a regulatory T-cell phenotype.
Buza
Joram J
JJ
College of Veterinary Medicine, Mississippi State, MS 39762, USA. buza@cvm.msstate.edu
Burgess
Shane C
SC
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002645:Chickens; D030562:Databases, Protein; D004195:Disease Models, Animal; D006801:Humans; D017730:Ki-1 Antigen / Q000378:metabolism*; D016399:Lymphoma, T-Cell / Q000737:chemistry* / Q000378:metabolism; D008380:Marek Disease; D008954:Models, Biological; D008969:Molecular Sequence Data; D020543:Proteome; D015398:Signal Transduction / Q000502:physiology
D017730:Ki-1 Antigen; D020543:Proteome