Drought tolerance and antioxidant activities in lavender plants colonized by native drought-tolerant or drought-sensitive Glomus Species.
This study compared the effectiveness of four arbuscular mycorrhizal (AM) fungal isolates (two autochthonous presumably drought-tolerant Glomus sp and two allochthonous presumably drought-sensitive strains) on a drought-adapted plant (Lavandula spica) growing under drought conditions. The autochthonous AM fungal strains produced a higher lavender biomass, specially root biomass, and a more efficient N and K absorption than with the inoculation of similar allochthonous strains under drought conditions. The autochthonous strains of Glomus intraradices and Glomus mosseae increased root growth by 35% and 100%, respectively, when compared to similar allochthonous strains. These effects were concomitant with an increase in water content and a decline in antioxidant compounds: 25% glutathione, 7% ascorbate and 15% H(2)O(2) by G. intraradices, and 108% glutathione, 26% ascorbate and 43% H(2)O(2) by G. mosseae. Glutathione and ascorbate have an important role in plant protection and metabolic function under water deficit; the low cell accumulation of these compounds in plants colonized by autochthonous AM fungal strains is an indication of high drought tolerance. Non-significant differences between antioxidant activities such as glutathione reductase (GR), catalase (CAT) and superoxide dismutase (SOD) in colonized plants were found. Thus, these results do not allow the generalization that GR, CAT and SOD were correlated with the symbiotic efficiency of these AM fungi on lavender drought tolerance. Plants colonized by allochthonous G. mosseae (the less efficient strain under drought conditions) had less N and K content than those colonized by similar autochthonous strain. These ions play a key role in osmoregulation. The AM symbiosis by autochthonous adapted strains also produced the highest intraradical and arbuscular development and extraradical mycelial having the greatest fungal SDH and ALP-ase activities in the root systems. Inoculation of autochthonous drought tolerant fungal strains is an important strategy that assured the greatest tolerance water stress contributing to the best lavender growth under drought.
Marulanda
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Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín (CSIC), Profesor Albareda no. 1, 18008, Granada, Spain.
Porcel
R
R
Barea
J M
JM
Azcón
R
R
D000222:Adaptation, Physiological; D000469:Alkaline Phosphatase / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism; D018533:Biomass; D004190:Disasters; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D027523:Lavandula / Q000254:growth & development* / Q000378:metabolism* / Q000382:microbiology; D038821:Mycorrhizae / Q000254:growth & development* / Q000502:physiology; D009584:Nitrogen / Q000378:metabolism; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology; D018520:Plant Shoots / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology; D011188:Potassium / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000378:metabolism; D014867:Water / Q000378:metabolism
D014867:Water; D006861:Hydrogen Peroxide; D013385:Succinate Dehydrogenase; D000469:Alkaline Phosphatase; D005978:Glutathione; D009584:Nitrogen; D001205:Ascorbic Acid; D011188:Potassium