title: Production and regulation of lipase activity from Penicillium restrictum in submerged and solid-state fermentations.
abstract: Different carbon (C) sources, mainly carbohydrates and lipids, have been screened for their capacity to support growth and lipase production by Penicillium restrictum in submerged fermentation (SmF) and in solid-state fermentation (SSF). Completely different physiological behaviors were observed after the addition of easily (oleic acid and glucose) and complex (olive oil and starch) assimilable C sources to the liquid and solid media. Maximal lipolytic activities (12.1 U/mL and 17.4 U/g) by P. restrictum were obtained with olive oil in SmF and in SSF, respectively. Biomass levels in SmF (12.2-14.1 mg/mL) and SSF (7.0-8.0 mg/g) did not varied greatly with the distinct C sources used. High lipase production (12.3 U/g) using glucose was only attained in SSF, perhaps due to the ability of this fermentation process to minimize catabolite repression.
authors: Biochemistry Department, Institute of Chemistry, Federal University of Rio de Janeiro, Centro de Tecnologia, Bloco A, Lab. 549-2, 21945-900 Rio de Janeiro, RJ, Brazil.
authors: Luciana A I
authors: LA
authors: de Azeredo
authors: Patrícia M
authors: PM
authors: Gomes
authors: Geraldo L
authors: GL
authors: Sant'Anna
authors: Leda R
authors: LR
authors: Castilho
authors: Denise M G
authors: DM
authors: Freire
mesh_terms: D004789:Enzyme Activation / Q000187:drug effects; D005285:Fermentation / Q000187:drug effects / Q000502:physiology*; D005947:Glucose / Q000494:pharmacology; D008049:Lipase / Q000378:metabolism*; D019301:Oleic Acid / Q000494:pharmacology; D000069463:Olive Oil; D010407:Penicillium / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D010938:Plant Oils / Q000494:pharmacology; D013213:Starch / Q000494:pharmacology
chemical_list: D000069463:Olive Oil; D010938:Plant Oils; D019301:Oleic Acid; D013213:Starch; D008049:Lipase; D005947:Glucose