Tomographic bioluminescence imaging with varying boundary conditions.
Three-dimensional bioluminescence imaging is an emerging technique that can be used to monitor molecular events in intact living systems. The inverse problem of 3D bioluminescence imaging does not have a unique solution because it requires reconstruction of a 3D source function from a 2D one. A novel approach that addresses this problem with the aid of a simple experimental setup and solves the uniqueness problem of the solution for a monochromatic measurement set is suggested here. The approach is verified numerically by reconstructing bioluminescent objects of various shapes embedded inside highly scattering media, such as biologiçal tissue.
Soloviev
Vadim Y
VY
Department of Computer Science, University College London, London, UK. vadimsoloviev@yahoo.com
D003198:Computer Simulation; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008027:Light; D008163:Luminescent Measurements / Q000379:methods*; D008164:Luminescent Proteins / Q000378:metabolism*; D008954:Models, Biological; D012542:Scattering, Radiation; D041622:Tomography, Optical / Q000379:methods*
D008164:Luminescent Proteins