Forensic mitochondrial DNA analysis of 116 casework skeletal samples.
Between February 1999 and May 2005, 116 DNA extractions were completed on skeletal remains from routine casework. Overall, at least a partial mitochondrial DNA (mtDNA) profile was obtained on 83.6% of samples. Skeletal remains fell into two general categories: (1) samples for body identifications submitted by law enforcement and (2) samples submitted to answer historical or family identity questions. Body identification cases were more likely to yield full mtDNA profiles, whereas historical cases were more likely to result in partial profiles. Overall, the ability to obtain a full or partial profile primarily reflects the difference in the average age and condition of the samples in these two categories and thus, difference in the quantity and quality of the DNA. Cremated remains were uniformly unsuccessful, whereas infant/fetal remains were uniformly successful. Heteroplasmy in skeletal remains was observed at a rate similar to that in hair ( approximately 10%). For body identification cases, skeletal remains had the same mtDNA profile as the accompanying reference sample in 50% of cases.
Nelson
Kimberlyn
K
Mitotyping Technologies, 2565 Park Center Boulevard, Suite 200, State College, PA 16801, USA.
Melton
Terry
T
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001842:Bone and Bones / Q000473:pathology; D002056:Burns / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D022801:Complementarity Determining Regions / Q000235:genetics; D053836:DNA Degradation, Necrotic; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000302:isolation & purification*; D018732:Forensic Anthropology; D006801:Humans; D007223:Infant; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014070:Tooth / Q000473:pathology
D022801:Complementarity Determining Regions; D017931:DNA Primers; D004272:DNA, Mitochondrial