Structure-based functional analysis of the replication protein of plasmid R6K: key amino acids at the pi/DNA interface.
In previous work, we characterized the bases in an iteron of plasmid R6K that are important for the binding of pi protein monomers and dimers. Here we investigate the following six amino acids of pi, encoded by pir, hypothesized to be important for DNA contact: Ser71, Try74, Gly131, Gly211, Arg225, and Arg254.
Kunnimalaiyaan
Selvi
S
Department of Bacteriology, University of Wisconsin, 420 Henry Mall, Madison, WI 53706, USA.
Rakowski
Sheryl A
SA
Filutowicz
Marcin
M
D000596:Amino Acids / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019281:Dimerization; D008957:Models, Genetic; D008958:Models, Molecular; D009154:Mutation; D010957:Plasmids / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D012093:Replicon / Q000235:genetics / Q000502:physiology
D000596:Amino Acids; D004268:DNA-Binding Proteins; D004247:DNA