title: In vivo imaging of oral mucositis in an animal model using optical coherence tomography and optical Doppler tomography.
abstract: OBJECTIVE
  To assess noninvasive optical coherence tomography (OCT) and optical Doppler tomography (ODT) for early detection and evaluation of chemotherapy-induced oral mucositis.
  
  
  METHODS
  Cheek pouches of 10 Syrian golden hamsters were imaged using OCT/ODT during development of chemotherapy-induced mucositis. I.p. injections of 5-fluorouracil and mechanical irritation induced oral lesions. At 2, 4, 7, and 11 days, one hamster was sacrificed and processed for histopathology. OCT images were visually examined; ODT results were semiquantified. Imaging data were compared with histologic findings.
  
  
  RESULTS
  During the development of mucositis, OCT/ODT identified the following events: (a) change in epithelial thickness (beginning on day 2), (b) loss of surface keratinized layer continuity (beginning on day 4), (c) loss of epithelial (day 4 onwards) and submucosal integrity (day 7 onwards), (d) changes in axial blood flow velocity (increased on days 2 and 4; decreased on day 7), and (e) changes in blood vessel size (diameter doubled on day 2; quadrupled on day 4; unchanged on day 7). The semiquantitative imaging-based scoring system identified the severity of mucositis as defined by histopathology. The combination of imaging criteria used allowed for the detection of early, intermediate, and late mucositic changes. Imaging data gave higher scores compared with clinical scores early on, suggesting that the imaging-based diagnostic scoring was more sensitive to early mucositic change than the clinical scoring system. Once mucositis was established, imaging and clinical scores converged.
  
  
  CONCLUSIONS
  OCT/ODT identified chemotherapy-induced oral changes before their clinical manifestation, and the proposed scoring system for oral mucositis was validated for the semiquantification of mucositic change.
authors: Beckman Laser Institute, University of California at Irvine, CA 92612, USA. pwsmith@uci.edu
authors: Petra
authors: P
authors: Wilder-Smith
authors: Marie J
authors: MJ
authors: Hammer-Wilson
authors: Jun
authors: J
authors: Zhang
authors: Qiang
authors: Q
authors: Wang
authors: Kathryn
authors: K
authors: Osann
authors: Zhongping
authors: Z
authors: Chen
authors: Harvey
authors: H
authors: Wigdor
authors: Joel
authors: J
authors: Schwartz
authors: Joel
authors: J
authors: Epstein
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity; D006224:Cricetinae; D004195:Disease Models, Animal; D005472:Fluorouracil / Q000633:toxicity*; D008647:Mesocricetus; D009061:Mouth Mucosa / Q000098:blood supply / Q000473:pathology; D013280:Stomatitis / Q000139:chemically induced / Q000175:diagnosis* / Q000000981:diagnostic imaging; D041623:Tomography, Optical Coherence; D014463:Ultrasonography
chemical_list: D005472:Fluorouracil