Multi-grams scale purification of xanthanolides from Xanthium macrocarpum. Centrifugal partition chromatography versus silica gel chromatography.
Plants of the Asteraceae family are known as a source of sesquiterpene lactones with interesting biological activities. The purification of several xanthanolides (xanthathin, 4-epi-xanthanol and 4-epi-isoxanthanol) was realized in one step, directly from the crude chloroformic extract of the leaves of X. macrocarpum by silica gel chromatography and for the first time by liquid/liquid chromatography (counter-current chromatography/centrifugal partition chromatography), using a FCPC 5L (Fast centrifugal partition chromatograph, Kromaton Technologies, Angers, France). Recovery, purity of xanthathin and solvent consumption were improved with the liquid/liquid chromatography compared to solid/liquid chromatography.
Pinel
Benoit
B
Laboratoire SONAS EA 921, UFR des Sciences Pharmaceutiques et Ingénierie de la Santé, 16 Bd Daviers, 49100 Angers, France.
Audo
Grégoire
G
Mallet
Sabine
S
Lavault
Marie
M
De La Poype
François
F
Séraphin
Denis
D
Richomme
Pascal
P
D002498:Centrifugation / Q000379:methods*; D002850:Chromatography, Gel / Q000379:methods*; D005663:Furans / Q000032:analysis / Q000737:chemistry*; D015394:Molecular Structure; D015203:Reproducibility of Results; D031210:Xanthium / Q000737:chemistry*
D005663:Furans; C022186:xanthatin