Haplotype-tagging interleukin-10 promoter polymorphism is associated with reduced risk of retinal artery occlusion.
OBJECTIVE
Pro- and anti-inflammatory cytokines, including interleukin 10 (IL-10), play an essential role in atherogenesis. Increased IL-10 production has been found among carriers of the IL10 [TCATA] haplotype, which is formed by five polymorphisms at position -3575, -2763, -1082, -819, and -592 in the promoter region of the IL10 gene. Due to linkage disequilibrium, the presence of the [TCATA] haplotype can be unequivocally determined by analysis of the IL10-592C>A polymorphism. The purpose of the present study was to investigate a hypothesized association between the haplotype-tagging IL10 -592C>A polymorphism and the presence of retinal artery occlusion (RAO).


METHODS
The present case-control study was comprised of 194 patients with RAO and 257 normal control subjects. Genotypes of the IL10 -592C>A polymorphism were determined by fluorogenic exonuclease (TaqMan) assay.


RESULTS
Carriers of the IL10 -592A-allele, indicating the presence of the IL10 [TCATA] haplotype, were found significantly more often in controls than among patients with RAO (48.6% versus 36.1%; p=0.008). In a logistic regression analysis after adjusting for age, sex, arterial hypertension, diabetes mellitus, hypercholesterolemia, and smoking habits, carriage of the IL10 -592A-allele was associated with an odds ratio of 0.65 (95% CI: 0.44-0.97) for RAO.


CONCLUSIONS
Our data suggest that the IL10 [TCATA] haplotype, identified by the presence of the IL10 -592A-allele, may exert a protective effect against RAO.
Weger
Martin
M
Department of Ophthalmology, Medical University of Graz, Austria. martin.weger@meduni-graz.at <martin.weger@meduni-graz.at>
Steinbrugger
Iris
I
El-Shabrawi
Yosuf
Y
Wegscheider
Beate Julia
BJ
Weger
Wolfgang
W
Renner
Wilfried
W
Schmut
Otto
O
Haas
Anton
A
D000225:Adenine; D000368:Aged; D016022:Case-Control Studies; D003596:Cytosine; D005260:Female; D005838:Genotype; D006239:Haplotypes; D006579:Heterozygote; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D015356:Retinal Artery Occlusion / Q000209:etiology*; D012189:Retrospective Studies; D012306:Risk
D016753:Interleukin-10; D003596:Cytosine; D000225:Adenine