title: Evidence for direct interactions between the mercuric ion transporter (MerT) and mercuric reductase (MerA) from the Tn501 mer operon.
abstract: Mercuric ion resistance in bacteria requires transport of mercuric ions (Hg(2+)) into the cytoplasmic compartment where they are reduced to the less toxic metallic mercury (Hg(0)) by mercuric reductase (MR). The long-established model for the resistance mechanism predicts interactions between the inner membrane mercuric ion transporter, MerT, and the N-terminal domain of cytoplasmic MR, but attempts to demonstrate this interaction have thus far been unsuccessful. A recently developed bacterial two-hybrid protein interaction detection system was used to show that the N-terminal region of MR interacts with the cytoplasmic face of MerT. We also show that the cysteine residues on the cytoplasmic face of the MerT protein are required for maximal mercuric ion transport but not for the interaction with mercuric reductase.
authors: School of Biosciences, The University of Birmingham, Edgbaston, Birmingham, UK.
authors: Mathieu
authors: M
authors: Schue
authors: Kerry J
authors: KJ
authors: Glendinning
authors: Jon L
authors: JL
authors: Hobman
authors: Nigel L
authors: NL
authors: Brown
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D027682:Cation Transport Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D008628:Mercury / Q000737:chemistry / Q000378:metabolism*; D009876:Operon; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D001426:Bacterial Proteins; D027682:Cation Transport Proteins; D004251:DNA Transposable Elements; D011994:Recombinant Proteins; C094115:mercury transport protein, bacterial; D010088:Oxidoreductases; C021551:mercuric reductase; D008628:Mercury