Mechanism of vitamin D3-induced transcription of phospholipase D1 in HaCat human keratinocytes.
1alpha,25-Dihydroxyvitamin D(3) (VitD(3)) increases protein and gene expression of phospholipase D1 (PLD1), but not PLD2, in HaCaT human keratinocytes. We show that VitD(3) increases PLD1 gene expression through a vitamin D responsive element (VDRE) on the 5' PLD1 promoter (-260 bp to -246 bp from exon 1). Similar results were obtained by transfecting VitD(3) receptor (VDR) into HEK293 cells, which are originally VitD(3)-unresponsive. Electrophoresis mobility shift assays (EMSA) and chromatin immunoprecipitation (CHIP) assays showed that the complex of VitD(3), VDR and retinoid X receptor alpha (RXRalpha) binds to the VDRE and increases PLD1 gene expression in HaCaT cells.
Kikuchi
Ryosuke
R
Nagoya University Graduate School of Medicine, Nagoya University School of Health Sciences, Daiko-minami 1-1-20, Higashi-ku, Nagoya, 461-8673, Japan.
Sobue
Sayaka
S
Murakami
Masashi
M
Ito
Hiromi
H
Kimura
Ami
A
Iwasaki
Takashi
T
Shibayama
Shuji
S
Takagi
Akira
A
Kojima
Tetsuhito
T
Suzuki
Motoshi
M
Banno
Yoshiko
Y
Nozawa
Yoshinori
Y
Murate
Takashi
T
D002478:Cells, Cultured; D002762:Cholecalciferol / Q000494:pharmacology*; D047369:Chromatin Immunoprecipitation; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism; D008954:Models, Biological; D010739:Phospholipase D / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000187:drug effects; D018167:Receptors, Calcitriol / Q000378:metabolism; D047490:Retinoid X Receptor alpha / Q000378:metabolism; D015533:Transcriptional Activation / Q000187:drug effects*; D030181:Vitamin D Response Element / Q000187:drug effects
D018167:Receptors, Calcitriol; D047490:Retinoid X Receptor alpha; D002762:Cholecalciferol; C105606:phospholipase D2; D010739:Phospholipase D; C106801:phospholipase D1