Case studies for transcriptional profiling.
DNA microarrays are frequently used to study transcriptome regulation in a wide variety of organisms. Although they are an invaluable tool for the acquisition of large scale dataset in plant systems biology, a number of surprising results and unanticipated complications are often encountered that illustrate the limitations and potential pitfalls of this technology. In this chapter we will present examples of real world studies from two classes of microarray experiments that were designed to (i) identify target genes for transcriptional regulators and (ii) to characterize complex expression patterns to reveal unexpected dependencies within transcriptional networks.
Hennig
Lars
L
Plant Biotechnology, Swiss Federal Institute of Technology Zürich, Universitätstr 2, 8092 Zürich, Switzerland. lhennig@ethz.ch
Köhler
Claudia
C
D000818:Animals; D017360:Arabidopsis / Q000235:genetics; D020869:Gene Expression Profiling; D017343:Genes, Plant / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D014158:Transcription, Genetic