Analysis of stress responsive genes induced by single-walled carbon nanotubes in BJ Foreskin cells.
Nanotechnology is finding its use as a potential technology in consumer products, defense, electronics, and medical applications by exploiting the properties of nanomaterials. Single-walled carbon nanotubes are novel forms of these nanomaterials with potential for large applications. However, the toxicity studies on this material are not explored in detail and therefore limiting its use. It has been earlier reported that single-walled carbon nanotubes induces oxidative stress and also dictates activation of specific signaling pathway in keratinocytes. The present study explores the effect of single-walled carbon nanotubes on stress genes in human BJ Foreskin cells. The results show induction of oxidative stress in BJ Foreskin cells by single-walled carbon nanotubes and increase in stress responsive genes. The genes included inducible genes like HMOX1, HMOX2, and Cyp1B1. In addition we validated increase for four genes by SWCNT, namely ATM, CCNC, DNAJB4, and GADD45A by RT-PCR. Moreover results of the altered stress related genes have been discussed and that partially explains some of the toxic responses induced by single-walled carbon nanotubes.
Sarkar
Shubhashish
S
Molecular Neurotoxicology Laboratory/Proteomics Core, Department of Biology, Texas Southern University, Houston, TX 77004, USA.
Sharma
Chidananda
C
Yog
Rajeshwari
R
Periakaruppan
Adaikkappan
A
Jejelowo
Olufisayo
O
Thomas
Renard
R
Barrera
Enrique V
EV
Rice-Ficht
Allison C
AC
Wilson
Bobby L
BL
Ramesh
Govindarajan T
GT
D002460:Cell Line; D004126:Dimethylformamide / Q000737:chemistry; D052816:Foreskin / Q000166:cytology*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects*; D007700:Kinetics; D008297:Male; D036103:Nanotechnology / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012997:Solvents / Q000737:chemistry
D037742:Nanotubes, Carbon; D017382:Reactive Oxygen Species; D012997:Solvents; D004126:Dimethylformamide