title: Intraperitoneal immunization with oligomannose-coated liposome-entrapped soluble leishmanial antigen induces antigen-specific T-helper type immune response in BALB/c mice through uptake by peritoneal macrophages.
abstract: The present study demonstrates that the intraperitoneal administration of soluble leishmanial antigen (SLA) entrapped in liposomes coated with neoglycolipids containing oligomannose residues (mannopentaose or mannotriose) strongly induces an antigen-specific T-helper type 1 (Th1) immune response in BALB/c mice. In response to in vitro stimulation with SLA, spleen cells from mice that had received oligomannose-coated liposomes encasing SLA (SLA-OML) displayed greater interferon (IFN)-gamma and interleukin (IL)-2 production and lower IL-4 and IL-5 production than spleen cells from mice that had received SLA alone, indicating that the SLA-specific Th1 immune response had predominantly been induced in the mice that had received SLA-OML. After subsequent infection with Leishmania major, mice that had received SLA-OML were effectively protected against the disease, with a predominant production of IFN-gamma. OML were preferentially and rapidly incorporated into peritoneal macrophages, and the transplantation of macrophages containing SLA-OML into the peritoneal cavity also induced protection against L. major infection. Thus, SLA-OML were shown to successfully induce a specific Th1 immune response capable of controlling L. major infection in BALB/c mice through the effective uptake of OML by peritoneal macrophages.
authors: Institute of Glycotechnology, Tokai University, Hiratsuka, Kanagawa 259-1292, Japan.
authors: Y
authors: Y
authors: Shimizu
authors: H
authors: H
authors: Takagi
authors: T
authors: T
authors: Nakayama
authors: K
authors: K
authors: Yamakami
authors: T
authors: T
authors: Tadakuma
authors: N
authors: N
authors: Yokoyama
authors: N
authors: N
authors: Kojima
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000008:administration & dosage / Q000276:immunology*; D039481:CD11b Antigen / Q000032:analysis; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D007114:Immunization; D018320:Leishmania major / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000517:prevention & control*; D008081:Liposomes; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D008358:Mannose / Q000008:administration & dosage*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009844:Oligosaccharides / Q000008:administration & dosage*; D016052:Protozoan Vaccines / Q000008:administration & dosage* / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology*
chemical_list: D000953:Antigens, Protozoan; D039481:CD11b Antigen; D016207:Cytokines; D008081:Liposomes; D009844:Oligosaccharides; D016052:Protozoan Vaccines; D008358:Mannose