title: [Experiment study on the diagnostic value of bile K-ras gene mutation responsible to the hepatic metastasis of pancreatic cancer].
abstract: OBJECTIVE
  To investigate the early diagnosis value of bile K-ras mutation responsible for hepatic metastasis of pancreatic cancer.
  
  
  METHODS
  While building the animal models with pancreatic cancer and hepatic metastasis of pancreatic cancer of rats, we collected bile from the model animals, and used the polymerase chain reaction and restriction fragment-length polymorphism (PCR-RFLP) to evaluate the point mutation at exon codon 12 of K-ras gene in bile. Then, the above result was compared with pathological slice.
  
  
  RESULTS
  The positive rates of K-ras point mutation in bile were 84.2% (16/19) for the model with hepatic metastasis of pancreatic cancer, and 0.0% (0/16) for the model with pancreatic cancer without hepatic metastasis. For the model with hepatic metastasis of pancreatic cancer, the sensitivity, specificity, positive prediction or negative prediction value of surveying K-ras mutation in bile was 84.2%, 85.7%, 84.2% or 85.7% respectively.
  
  
  CONCLUSIONS
  Both the mutability and the specificity of K-ras gene mutation at codon 12 in bile are high. Our above work can supply a new elemental filter method for the early diagnosis of hepatic metastasis of pancreatic cancer.
authors: Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
authors: Xiang
authors: X
authors: Zhou
authors: Zhao-Da
authors: ZD
authors: Zhang
authors: Bo-Le
authors: BL
authors: Tian
authors: Fang-Hai
authors: FH
authors: Han
authors: Wei-Ming
authors: WM
authors: Hu
authors: Wei
authors: W
authors: Jia
authors: Hui-Ming
authors: HM
authors: Lu
mesh_terms: D000818:Animals; D001646:Bile / Q000378:metabolism*; D045744:Cell Line, Tumor; D003062:Codon / Q000235:genetics; D005091:Exons / Q000235:genetics; D005260:Female; D011905:Genes, ras / Q000235:genetics*; D008113:Liver Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000556:secondary*; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology*; D017354:Point Mutation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D003062:Codon