title: Purification, characterization, gene cloning, and expression of a novel alcohol dehydrogenase with anti-prelog stereospecificity from Candida parapsilosis.
abstract: An alcohol dehydrogenase from Candida parapsilosis CCTCC M203011 was characterized along with its biochemical activity and structural gene. The amino acid sequence shows similarity to those of the short-chain dehydrogenase/reductases but no overall identity to known proteins. This enzyme with unusual stereospecificity catalyzes an anti-Prelog reduction of 2-hydroxyacetophenone to (S)-1-phenyl-1,2-ethanediol.
authors: Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Southern Yangtze University, Wuxi 214122, People's Republic of China.
authors: Yao
authors: Y
authors: Nie
authors: Yan
authors: Y
authors: Xu
authors: Xiao Qing
authors: XQ
authors: Mu
authors: Hai Yan
authors: HY
authors: Wang
authors: Ming
authors: M
authors: Yang
authors: Rong
authors: R
authors: Xiao
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D002175:Candida / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013237:Stereoisomerism; D013379:Substrate Specificity
chemical_list: D004271:DNA, Fungal; D000426:Alcohol Dehydrogenase