title: Polyamines in spermatocytes and residual bodies of rat testis.
abstract: Immunocytochemistry for polyamines in the rat testis revealed intense staining of small bodies close to the lumen of seminiferous tubules of spermatogenic stage VII and VIII as well as of spermatocytes. Methyl green-pyronin and propidium iodide staining combined with DNase or RNase predigestion showed that the small bodies contained RNA, but not DNA and double fluorescence staining showed that polyamines (PAs) colocalized with RNA in the bodies. Electron microscopy confirmed the absence of nuclei in the bodies and revealed that PA immunoreactivity was associated with ribosomes. These results strongly suggest that the small bodies correspond to the residual bodies, and agree with previous results showing localization of PAs to ribosomes in neurons and gastrointestinal epithelial cells. The accumulation of PAs in residual bodies may reflect a termination of their role in spermiogenesis with respect to protein synthesis.
authors: Department of Applied Life Science, Faculty of Biotechnology and Life Science, Sojo University, Ikeda 4-22-1, Kumamoto, 860-0082, Japan.
authors: Masashi
authors: M
authors: Shin
authors: Lars-Inge
authors: LI
authors: Larsson
authors: Kunio
authors: K
authors: Fujiwara
mesh_terms: D000818:Animals; D004396:Coloring Agents / Q000737:chemistry; D019084:Fluorescent Antibody Technique, Indirect; D008297:Male; D008739:Methyl Green / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D016253:Microscopy, Immunoelectron; D011073:Polyamines / Q000378:metabolism*; D011754:Pyronine / Q000737:chemistry; D012313:RNA / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012270:Ribosomes / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D013090:Spermatocytes / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D013194:Staining and Labeling; D013737:Testis / Q000166:cytology / Q000378:metabolism*
chemical_list: D004396:Coloring Agents; D011073:Polyamines; D012313:RNA; D008739:Methyl Green; D011754:Pyronine