Vitrification of human laser treated blastocysts within cut standard straws (CSS): novel aseptic packaging and reduced concentrations of cryoprotectants.
Vitrification of laser treated human blastocysts using reduced concentrations of permeable cryoprotectants was carried out by submerging cut standard straws (CSS) into liquid nitrogen. The CSS were made by cutting a standard 0.25 ml straw at an angle of approximately 45 degrees . After laser assisted hatching, 6 day blastocysts (n=250) were loaded into droplets of approximately 0.75 microl in the CSS and were either plunged directly into liquid nitrogen or first encased in a standard 0.5 ml straw (aseptic technique) before being vitrified. Permeable cryoprotectants (ethylene glycol+Me(2)SO) at concentrations of 15% and 20% v:v were tested for their effect on post warming re-expansion and post transfer pregnancy rates. Our results indicate that the use of reduced concentrations of cryoprotectants and aseptic packaging of blastocysts did not have any statistically significant impact on the study outcomes.
Isachenko
Vladimir
V
IVF Clinic AltraVita, Moscow, Russia. v.isachenko@yahoo.com
Katkov
Igor I
II
Yakovenko
Sergey
S
Lulat
Ayub G-M I
AG
Ulug
Murat
M
Arvas
Ayse
A
Isachenko
Evgenia
E
D001755:Blastocyst / Q000528:radiation effects*; D015925:Cryopreservation / Q000295:instrumentation* / Q000379:methods; D003451:Cryoprotective Agents / Q000008:administration & dosage*; D004121:Dimethyl Sulfoxide / Q000008:administration & dosage; D019855:Ethylene Glycol / Q000008:administration & dosage; D006801:Humans; D007834:Lasers
D003451:Cryoprotective Agents; D019855:Ethylene Glycol; D004121:Dimethyl Sulfoxide