title: Wilms' tumor gene (WT1) is predominantly expressed in clonal hematopoietic cells in myelodysplastic syndromes.
abstract: To determine the expression of Wilms' tumor gene (WT1) in clonal hematopoietic cells in patients with myelodysplastic syndromes (MDS), immunochemistry labelling (alkaline phosphatase anti-alkaline phosphatase) and fluorescence in situ hybridization (FISH) were coperformed on bone marrow cytospins from 18 patients whose abnormal karyotypes had been determined by G-binding analysis. Compared to 12 healthy donors, WT1 positive nucleated cells in MDS marrows were significantly higher (t = 2.30; P = 0.032). Moreover, WT1 was expressed predominantly in MDS clonal cells (with abnormal FISH signals) rather than in non-clonal cells (residual normal cells) (t = 2.19; P = 0.043).
authors: Department of Hematology, The Sixth People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai, China. lixiao3326@yahoo.com.cn
authors: Xiao
authors: X
authors: Li
authors: Lingyun
authors: L
authors: Wu
authors: Shaoxu
authors: S
authors: Ying
authors: Chunkang
authors: C
authors: Chang
authors: Quan
authors: Q
authors: Pu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000753:Anemia, Refractory / Q000235:genetics / Q000378:metabolism; D000754:Anemia, Refractory, with Excess of Blasts; D016022:Case-Control Studies; D002869:Chromosome Aberrations; D002999:Clone Cells; D005260:Female; D015870:Gene Expression; D006413:Hematopoietic System / Q000378:metabolism; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics* / Q000378:metabolism; D025721:WT1 Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D025721:WT1 Proteins