[Purification of human VLDL apolipoproteins by middle pressure liquid chromatography].
OBJECTIVE
To purify human VLDL apolipoproteins by middle-pressure liquid chromatography.


METHODS
Human VLDLs were isolated by one step density ultracentrifugation. Delipided human VLDL was separated by Sephacryl S-200 molecular sieve chromatography. ApoE was purified by heparin Sepharose CL-6B affinity chromatography. ApoC I ,C II and C III were purified from apoC. fraction by DEAE-Sephacel ion exchange chromatography:


RESULTS
Purified apoE, apoC I, apoC II and apoC III were obtained. SDS-PAGE and immunodiffusion tests indicated the isolated proteins were pure.


CONCLUSIONS
We have established a purification procedure for human VLDL apolipoproteins with highly efficiency and simplicity by MPLC.
Xin
Yan-Fei
YF
Apolipoprotein Research Unit, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Duan
Yun
Y
Deng
Zu-Yue
ZY
Zhang
Zu-Hui
ZH
Liu
Bing-Wen
BW
D001053:Apolipoproteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D005779:Immunodiffusion; D008079:Lipoproteins, VLDL / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D011312:Pressure; D012995:Solubility; D013997:Time Factors
D001053:Apolipoproteins; D008079:Lipoproteins, VLDL