title: MTOC reorientation occurs during FcgammaR-mediated phagocytosis in macrophages.
abstract: Cell polarization is essential for targeting signaling elements and organelles to active plasma membrane regions. In a few specialized cell types, cell polarity is enhanced by reorientation of the MTOC and associated organelles toward dynamic membrane sites. Phagocytosis is a highly polarized process whereby particles >0.5 microm are internalized at stimulated regions on the cell surface of macrophages. Here we provide detailed evidence that the MTOC reorients toward the site of particle internalization during phagocytosis. We visualized MTOC proximity to IgG-sRBCs in fixed RAW264.7 cells, during live cell imaging using fluorescent chimeras to label the MTOC and using frustrated phagocytosis assays. MTOC reorientation in macrophages is initiated by FcgammaR ligation and is complete within 1 h. Polarization of the MTOC toward the phagosome requires the MT cytoskeleton and dynein motor activity. cdc42, PI3K, and mPAR-6 are all important signaling molecules for MTOC reorientation during phagocytosis. MTOC reorientation was not essential for particle internalization or phagolysosome formation. However Golgi reorientation in concert with MTOC reorientation during phagocytosis implicates MTOC reorientation in antigen processing events in macrophages.
authors: Department of Cell and Systems Biology, University of Toronto at Scarborough, Toronto, Ontario, Canada.
authors: Edward W
authors: EW
authors: Eng
authors: Adam
authors: A
authors: Bettio
authors: John
authors: J
authors: Ibrahim
authors: Rene E
authors: RE
authors: Harrison
mesh_terms: D000199:Actins / Q000378:metabolism; D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000818:Animals; D016764:Cell Polarity; D004398:Dyneins / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D022101:Microtubule-Organizing Center / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism; D008954:Models, Biological; D010587:Phagocytosis; D010588:Phagosomes / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D011961:Receptors, Fc / Q000378:metabolism*; D012756:Sheep; D015398:Signal Transduction; D020764:cdc42 GTP-Binding Protein / Q000378:metabolism
chemical_list: D000199:Actins; D048868:Adaptor Proteins, Signal Transducing; C518597:Par6 protein, mouse; D011961:Receptors, Fc; D019869:Phosphatidylinositol 3-Kinases; D004398:Dyneins; D020764:cdc42 GTP-Binding Protein