title: Quantification of buserelin in a pharmaceutical product by multiple-injection CZE.
abstract: An efficient and rapid separation method based on reversed-polarity multiple-injection CZE (MICZE), has been developed for the quantification of buserelin in a pharmaceutical product. The determinations were performed by serially injecting five standard solutions of buserelin (50-300 microg/mL) and one reference analyte into a Polybrene-coated capillary. All the samples contained goserelin, an analog peptide to buserelin, as internal standard (IS). Immediately after pressure injection, the applied sample plugs were subjected to electrophoresis for 2 min at -25 kV. Consequently, each sample plug became isolated from its neighboring plugs by the BGE, composed of 100 mM phosphate-triethanolamine buffer at pH 3.0 containing 10% v/v ACN. During separation the individual sample components migrated at similar velocities and as distinct zones through the capillary giving 24 peaks, 12 from the analyte and the IS and 12 from the sample matrix. The buserelin content of the pharmaceutical product was determined to be 0.94 +/- 0.05 mg/mL, which is only a slight deviation from the declared concentration (1 mg/mL).
authors: Uppsala University, Division of Analytical Pharmaceutical Chemistry, Biomedical Centre, Uppsala, Sweden.
authors: Henrik
authors: H
authors: Lodén
authors: Ahmad
authors: A
authors: Amini
mesh_terms: D002064:Buserelin / Q000032:analysis*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation* / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry; D017273:Goserelin / Q000032:analysis; D006583:Hexadimethrine Bromide / Q000737:chemistry; D007267:Injections / Q000379:methods; D007525:Isoelectric Focusing / Q000379:methods; D013058:Mass Spectrometry; D004364:Pharmaceutical Preparations / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D020099:Coated Materials, Biocompatible; D004364:Pharmaceutical Preparations; D017273:Goserelin; D006583:Hexadimethrine Bromide; D002064:Buserelin