Reconstitution of the water-oxidizing complex in manganese-depleted photosystem II preparations using synthetic binuclear Mn(II) and Mn(IV) complexes: production of hydrogen peroxide.
Reconstitution of Mn-depleted PSII particles with synthetic binuclear Mn complexes (one Mn(II)(2) complex and one Mn(IV)(2) complex) was examined. In both cases the electron-transfer rates in the reconstituted systems were found to be up to 75-82% of that measured in native PSII but the oxygen evolution activity remained lower (<5-40%). However, hydrogen peroxide was also produced by the reconstituted samples. These samples therefore represent a new type of reconstituted PSII that generates hydrogen peroxide as the final product in reconstituted PSII centers.
Nagata
Toshi
T
Research Center for Molecular-scale Nanoscience, Institute for Molecular Science (IMS), National Institutes for Natural Sciences (NINS), 5-1 Higashiyama, Myodaiji, Okazaki 444-8787, Japan. toshi-n@ims.ac.jp
Nagasawa
Takayuki
T
Zharmukhamedov
Sergei K
SK
Klimov
Vyacheslav V
VV
Allakhverdiev
Suleyman I
SI
D015086:2,6-Dichloroindophenol / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D005453:Fluorescence; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007700:Kinetics; D008027:Light; D008345:Manganese / Q000172:deficiency* / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000528:radiation effects; D010100:Oxygen / Q000378:metabolism; D018532:Pisum sativum / Q000378:metabolism* / Q000528:radiation effects; D010788:Photosynthesis / Q000528:radiation effects; D045332:Photosystem II Protein Complex / Q000737:chemistry / Q000378:metabolism*; D014867:Water / Q000378:metabolism*
D045332:Photosystem II Protein Complex; D014867:Water; D002734:Chlorophyll; D008345:Manganese; D006861:Hydrogen Peroxide; D015086:2,6-Dichloroindophenol; D010100:Oxygen