[Erythromycin effects on apoptosis of epithelial cell in nasal polyps].
OBJECTIVE
To study the effect of erythromycin on apoptosis in epithelial cell and investigate the significance of epithelial cell apoptosis in nasal polyps forming.


METHODS
Epithelial cell collected from thirty nasal polyps and six inferior turbinates were cultured in Dulbecco Eagle and Ham F12 (1:1) and divided into two groups, one cultured with Erythromycin(Erythromycin group), another cultured without Erythromycin (control group). Apoptosis was detected by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling.


RESULTS
The AI (apoptosis index) of epithelial cell in nasal polyps after cultured for 1,3,5 days with erythromycin were respectively (33.23 +/- 6.50)%, (38.21 +/- 7.22)% and (52.63 +/- 7.86)%. The AI of epithelial cell in inferior turbinates were respectively (31.02 +/- 5.60)%, (32.13 +/- 7.15)% and (39.64 +/- 7.48)%. There were significant difference between two groups at 5 day after culture (P < 0.05).


CONCLUSIONS
Erythromycin promoted apoptosis of epithelial cell in nasal polyps.
Yang
Lei
L
Biotechique Center of Wenzhou Medical College, Wenzhou, 325000, China. yeleye@sohu.com
Xu
Sainan
S
Jin
Yongguo
Y
D017209:Apoptosis / Q000187:drug effects*; D004847:Epithelial Cells / Q000187:drug effects*; D004917:Erythromycin / Q000494:pharmacology*; D006801:Humans; D009298:Nasal Polyps / Q000473:pathology*
D004917:Erythromycin