Multifunctional chondroitin sulphate for cartilage tissue-biomaterial integration.
A biologically active, high-strength tissue adhesive is needed for numerous medical applications in tissue engineering and regenerative medicine. Integration of biomaterials or implants with surrounding native tissue is crucial for both immediate functionality and long-term performance of the tissue. Here, we use the biopolymer chondroitin sulphate (CS), one of the major components of cartilage extracellular matrix, to develop a novel bioadhesive that is readily applied and acts quickly. CS was chemically functionalized with methacrylate and aldehyde groups on the polysaccharide backbone to chemically bridge biomaterials and tissue proteins via a twofold covalent link. Three-dimensional hydrogels (with and without cells) bonded to articular cartilage defects. In in vitro and in vivo functional studies this approach led to mechanical stability of the hydrogel and tissue repair in cartilage defects.
Wang
Dong-An
DA
Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA.
Varghese
Shyni
S
Sharma
Blanka
B
Strehin
Iossif
I
Fermanian
Sara
S
Gorham
Justin
J
Fairbrother
D Howard
DH
Cascio
Brett
B
Elisseeff
Jennifer H
JH
D001672:Biocompatible Materials; D002357:Cartilage Diseases / Q000378:metabolism* / Q000503:physiopathology / Q000628:therapy*; D002358:Cartilage, Articular / Q000502:physiology*; D002809:Chondroitin Sulfates / Q000502:physiology*; D020100:Hydrogels; D014014:Tissue Adhesives; D023822:Tissue Engineering / Q000379:methods* / Q000639:trends*
D001672:Biocompatible Materials; D020100:Hydrogels; D014014:Tissue Adhesives; D002809:Chondroitin Sulfates