Preparative isolation and purification of triterpene saponins from Clematis mandshurica by high-speed counter-current chromatography coupled with evaporative light scattering detection.
Preparative high-speed counter-current chromatography (HSCCC) coupled with evaporative light scattering detection (ELSD) was successfully applied to the isolation and purification of four triterpene saponins from Clematis mandshurica by one-step separation. The solvent system was composed of ethyl acetate-n-butanol-ethanol-0.05% TFA (5:10:2:20, v/v). The purities of all the saponins obtained were better than 97%. The structures of the compounds were elucidated by the means of spectroscopic methods.
Shi
Shepo
S
School of Pharmaceutical Sciences, Peking University Health Science Center, No. 38 Xueyuan Road, Beijing 100083, China.
Jiang
Dan
D
Zhao
Mingbo
M
Tu
Pengfei
P
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D039324:Clematis / Q000737:chemistry*; D003377:Countercurrent Distribution / Q000379:methods*; D008027:Light; D008969:Molecular Sequence Data; D012015:Reference Standards; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification*; D012542:Scattering, Radiation; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification*
D012503:Saponins; D014315:Triterpenes