Preparative separation of isomeric and stereoisomeric dicarboxylic acids by pH-zone-refining counter-current chromatography.
This work involves the preparative separation of some isomeric dicarboxylic acids using pH-zone-refining counter-current chromatography (CCC), a relatively new preparative technique for the separation of ionizable compounds. The paper concentrates especially on the separation of a synthetic mixture of closely related cis and trans pairs of 1-methyl- and 1,3-dimethyl-1,3-cyclohexanedicarboxylic acids. The elution sequence of the isomers is discussed in terms of their relative acidities (pK(a) values) in solution and gas phase, hydrophobicities, and steric configuration. Two possible explanations are suggested for the mechanism of separation. They both involve the amount of retainer acid used, as it affects the separation and plays a role in the chemohydrodynamic equilibrium of the dicarboxylic acids in the column.
Weisz
Adrian
A
Office of Cosmetics and Colors, Center for Food Safety and Applied Nutrition, US Food and Drug Administration, College Park, MD 20740, USA. adrianweisz@fda.hhs.gov
Idina
Ana
A
Ben-Ari
Julius
J
Karni
Miriam
M
Mandelbaum
Asher
A
Ito
Yoichiro
Y
D003377:Countercurrent Distribution / Q000379:methods*; D003998:Dicarboxylic Acids / Q000737:chemistry* / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D013237:Stereoisomerism
D003998:Dicarboxylic Acids