title: Influence of different ECM mimetic peptide sequences embedded in a nonfouling environment on the specific adhesion of human-skin keratinocytes and fibroblasts on deformable substrates.
abstract: Mechanical stress is a decisive factor for the differentiation, proliferation, and general behavior of cells. However, the specific signaling of mechanotransduction is not fully understood. One basic problem is the clear distinction between the different extracellular matrix (ECM) constituents that participate in cellular adhesion and their corresponding signaling pathways. Here, a system is proposed that enables mechanical stimulation of human-skin-derived keratinocytes and human dermal fibroblasts that specifically interact with peptide sequences immobilized on a non-interacting but deformable substrate. The peptide sequences mimic fibronectin, laminin, and collagen type IV, three major components of the ECM. To achieve this, PDMS is activated using ammonia plasma and coated with star-shaped isocyanate-terminated poly(ethylene glycol)-based prepolymers, which results in a functional coating that prevents unspecific cell adhesion. Specific cell adhesion is achieved by functionalization of the layers with the peptide sequences in different combinations. Moreover, a method that enables the decoration of deformable substrates with cell-adhesion peptides in extremely defined nanostructures is presented. The distance and clustering of cell adhesion molecules below 100 nm has been demonstrated to be of utmost importance for cell adhesion. Thus we present a new toolbox that allows for the detailed analysis of the adhesion of human-skin-derived cells on structurally and biochemically decorated deformable substrates.
authors: DWI an der RWTH Aachen e.V. Pauwelsstr. 8, 52074 Aachen, Germany.
authors: Jochen
authors: J
authors: Salber
authors: Stefan
authors: S
authors: Gräter
authors: Marc
authors: M
authors: Harwardt
authors: Matthias
authors: M
authors: Hofmann
authors: Doris
authors: D
authors: Klee
authors: Jadranka
authors: J
authors: Dujic
authors: Huang
authors: H
authors: Jinghuan
authors: Jiandong
authors: J
authors: Ding
authors: Stefan
authors: S
authors: Kippenberger
authors: August
authors: A
authors: Bernd
authors: Jürgen
authors: J
authors: Groll
authors: Joachim P
authors: JP
authors: Spatz
authors: Martin
authors: M
authors: Möller
mesh_terms: D000595:Amino Acid Sequence; D040761:Biomimetic Materials / Q000737:chemistry*; D002448:Cell Adhesion; D002452:Cell Count; D002478:Cells, Cultured; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D005109:Extracellular Matrix / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology*; D006046:Gold; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D015603:Keratinocytes / Q000166:cytology*; D053758:Nanoparticles; D010455:Peptides / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D012828:Silicones / Q000737:chemistry; D012867:Skin / Q000166:cytology*
chemical_list: D004129:Dimethylpolysiloxanes; D010455:Peptides; D012828:Silicones; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols; C013830:baysilon; D006046:Gold