Helium-neon laser irradiation stimulates cell proliferation through photostimulatory effects in mitochondria.
Previous reports have shown that cellular functions could be influenced by visual light (400-700 nm). Recent evidence indicates that cellular proliferation could be triggered by the interaction of a helium-neon laser (He-Ne laser, 632.8 nm) with the mitochondrial photoacceptor-cytochrome c oxidase. Our previous studies demonstrated that He-Ne irradiation induced an increase in cell proliferation, but not migration, in the melanoma cell line A2058 cell. The aim of this study was to investigate the underlying mechanisms involved in photostimulatory effects induced by an He-Ne laser. Using the A2058 cell as a model for cell proliferation, the photobiologic effects induced by an He-Ne laser were studied. He-Ne irradiation immediately induced an increase in mitochondrial membrane potential (delta psi(mt)), ATP, and cAMP via enhanced cytochrome c oxidase activity and promoted phosphorylation of Jun N-terminal kinase (JNK)/activator protein-1 (AP-1) expressions. He-Ne irradiation-induced A2058 cell proliferation was significantly abrogated by the addition of delta psi(mt) and JNK inhibitors. Moreover, treatment with an He-Ne laser resulted in delayed effects on IL-8 and transforming growth factor-beta1 release from A2058 cells. These results suggest that He-Ne irradiation elicits photostimulatory effects in mitochondria processes, which involve JNK/AP-1 activation and enhanced growth factor release, and ultimately lead to A2058 cell proliferation.
Hu
Wan-Ping
WP
Faculty of Biotechnology and Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
Wang
Jeh-Jeng
JJ
Yu
Chia-Li
CL
Lan
Cheng-Che E
CC
Chen
Gow-Shing
GS
Yu
Hsin-Su
HS
D000255:Adenosine Triphosphate / Q000032:analysis; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000528:radiation effects*; D000242:Cyclic AMP / Q000032:analysis; D003576:Electron Transport Complex IV / Q000032:analysis; D006371:Helium / Q000494:pharmacology*; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D019394:Ki-67 Antigen / Q000032:analysis; D007834:Lasers; D008545:Melanoma / Q000473:pathology; D008564:Membrane Potentials / Q000528:radiation effects; D008928:Mitochondria / Q000502:physiology / Q000528:radiation effects*; D009356:Neon / Q000494:pharmacology*; D010766:Phosphorylation; D018808:Transcription Factor AP-1 / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis
D016209:Interleukin-8; D019394:Ki-67 Antigen; D018808:Transcription Factor AP-1; D053773:Transforming Growth Factor beta1; D006371:Helium; D009356:Neon; D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D003576:Electron Transport Complex IV; D048031:JNK Mitogen-Activated Protein Kinases