title: Fourier transform infrared imaging as a method for detection of HLA class I expression in melanoma without the use of antibody.
abstract: Human leukocyte antigen (HLA) class I expression in melanoma is usually assessed using immunohistochemical staining. Here we report on the use of Fourier transform infrared (FTIR) hyperspectral imaging, a method widely used in two-dimensional analysis of chemical components, to study HLA class I expression in tissue. Two-dimensional cluster colour images derived from unsupervised hierarchical cluster analysis of FTIR hyperspectral data on melanoma sections were compared with consecutive sections that were immunohistochemically stained for class I expression. HLA-class-I-positive and -negative areas were differentiated by FTIR cluster images in all eight melanoma sections investigated without the need for antibody attachment. FTIR imaging enables the distinction of HLA-class-I-positive from class-I-negative areas in melanoma. This method is accurate, rapid and cost-effective.
authors: Centre for Biospectroscopy and School of Chemistry, Monash University, Victoria 3800, Australia.
authors: S F
authors: SF
authors: Chew
authors: B R
authors: BR
authors: Wood
authors: C
authors: C
authors: Kanaan
authors: J
authors: J
authors: Browning
authors: D
authors: D
authors: MacGregor
authors: I D
authors: ID
authors: Davis
authors: J
authors: J
authors: Cebon
authors: B D
authors: BD
authors: Tait
authors: D
authors: D
authors: McNaughton
mesh_terms: D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D006801:Humans; D008545:Melanoma / Q000175:diagnosis* / Q000378:metabolism; D012878:Skin Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D015395:Histocompatibility Antigens Class I