[Genetic polymorphisms at five STR loci in Chengdu Han population].
OBJECTIVE
To understand the allele structure and genetic polymorphisms at five STR loci in Chengdu Han population, to obtain a preliminary database and to analyze the value in forensic medicine.


METHODS
EDTA-blood specimens were collected from unrelated individuals. The DNA of samples was extracted with Chelex-100 method. And then the PCR amplification, PAGE horizontal electrophoresis and gel silver staining were performed for analyzing the polymorphisms of five STR loci in Chengdu Han population.


RESULTS
In the five STR loci,10, 7, 5, 7 and 7 alleles were found respectively. The observed heterozygosity of 83%, 80%, 64%, 66% and 77% and discrimination power of 95.44%, 91.0%, 84.3%, 86.1% and 86.8% were identified for the five STR loci respectively. All loci obeyed Hardy-Weinberg equilibrium.


CONCLUSIONS
The five STR loci display the high discrimination power. The obtained data are desirable for forensic analysis and benefiting to the research of the population genetics.
Chen
Xiao
X
Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Song
Yan-Hua
YH
Li
Ying
Y
Liao
Miao
M
Long
Bin
B
Chen
Guo-Di
GD
D044466:Asian People / Q000208:ethnology* / Q000235:genetics*; D002681:China / Q000208:ethnology; D004586:Electrophoresis; D005006:Ethnicity / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D056426:Genetic Loci / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*