[Effects of Ginkgo biloba extract (ginaton) on mRNA expression of bcl-2 and bcl-xL in myocardium of patients underwent hypothermic cardiopulmonary bypass].
OBJECTIVE
To investigate the effects of Ginkgo biloba extract (Ginaton) on bcl-2 and bcl-xL mRNA expression in the myocardium of patients underwent hypothermic cardiopulmonary bypass (CPB).


METHODS
Thirty congenital heart disease patients were randomly assigned to 2 groups, the control group and the treated group. Patients in both groups received St. Thomas' cardioplegic solution via radix aortae, while Ginaton (0.5 mg/kg) was added in the treated group. Cardiac surgery was started after complete heart arrest. Myocardium was taken before the aorta ascendens was unblocked and mRNA expression of bcl-2 and bcl-xL in the ventricular tissue was detected by RT-PCR.


RESULTS
The gene expressions of bcl-2 and bcl-xL were significantly higher in the treated group than those in the control group (P < 0.05).


CONCLUSIONS
Ginaton could promote the mRNA expressions of the antiapoptotic gene bcl-2 and bcl-xL in myocardium of patients underwent CI'PB.
Deng
Yun-kun
YK
Department of Anesthesiology, Guizhou Provincial People's Hospital, Guiyang. dykwf@yahoo.com
Wei
Fang
F
Li
Yong-nian
YN
D002315:Cardiopulmonary Bypass / Q000379:methods; D002648:Child; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D020441:Ginkgo biloba / Q000737:chemistry*; D006330:Heart Defects, Congenital / Q000235:genetics / Q000601:surgery; D006801:Humans; D007036:Hypothermia, Induced; D008297:Male; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D018515:Plant Leaves / Q000737:chemistry; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051020:bcl-X Protein / Q000235:genetics*
D004365:Drugs, Chinese Herbal; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D051020:bcl-X Protein; C526713:ginaton