title: Clinical models that objectively evaluate interproximal plaque based on total protein concentration.
abstract: OBJECTIVE
  To establish clinical models that accurately and objectively assess the levels of interproximal plaque (IPP) by measuring the total protein concentration.
  
  
  METHODS
  The multiple treatment (MTM) and single-treatment models (STM) were designed as randomized, double-blinded, crossover studies whereby IPP was collected using floss. The total protein concentration in the plaque was then measured using a fluorescence microplate assay. To demonstrate sensitivity, the levels of residual IPP were compared for two distinctly different plaque removal routines: brushing versus brushing+flossing.
  
  
  RESULTS
  Eighteen subjects completed the study for the MTM, while 16 participants completed the study for the STM. The results showed that both models were able to distinguish between treatments. Moreover, fundamental profiles were established that describe the mean changes in the IPP concentration over time and by treatment. Finally, a comparison between our results and a direct measure of IPP, i.e., plaque weight, demonstrated the potential of using protein as a quantitative means to assess IPP levels.
  
  
  CONCLUSIONS
  The MTM and STM provide an objective, quantitative assessment of IPP based on the total protein concentration in plaque.
authors: Philips Oral Healthcare, Snoqualmie, WA 98065, USA. nick.milanovich@philips.com
authors: N
authors: N
authors: Milanovich
authors: S M
authors: SM
authors: Souza
authors: M
authors: M
authors: de Jager
authors: A F
authors: AF
authors: Hefti
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D018592:Cross-Over Studies; D003742:Dental Devices, Home Care; D003773:Dental Plaque / Q000737:chemistry / Q000145:classification* / Q000628:therapy; D003774:Dental Plaque Index; D004311:Double-Blind Method; D005450:Fluorescamine; D005453:Fluorescence; D006801:Humans; D007202:Indicators and Reagents; D008875:Middle Aged; D011506:Proteins / Q000032:analysis*; D012648:Self Care; D013997:Time Factors; D014070:Tooth; D014099:Toothbrushing / Q000295:instrumentation
chemical_list: D007202:Indicators and Reagents; D011506:Proteins; D005450:Fluorescamine