Illuminating molecular processes in living cells.
Lately, scientists have explored approaches to developing fluorescent and/or bioluminescent indicators to pinpoint cellular processes in single living cells. These analytical methods have become a key technology for visualizing and detecting what was otherwise unseen in live cells. The target signaling included second messengers, protein phosphorylations, protein-protein interactions, and protein localizations.
Umezawa
Yoshio
Y
Department of Chemistry, School of Science, The University of Tokyo, Hongo, Bunkyo-Ku, Tokyo 113-0033, Japan. umezawa@chem.s.u-tokyo.ac.jp
D000818:Animals; D016466:CHO Cells; D002468:Cell Physiological Phenomena; D006224:Cricetinae; D003412:Cricetulus; D004727:Endothelium / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D008954:Models, Biological; D015336:Molecular Probe Techniques; D010766:Phosphorylation; D025941:Protein Interaction Mapping; D015398:Signal Transduction
D005456:Fluorescent Dyes