Diversity of environmental Mycobacterium isolates from hemodialysis water as shown by a multigene sequencing approach.
Here we used a multigene sequencing approach for the identification and molecular typing of environmental mycobacteria of the fast-growing subgroup. Strains were isolated from hemodialysis water and clinical samples. Eleven type strains of related species of the genus were also included in this study. To gain further insight into the diversity of the environmental mycobacteria, we analyzed several housekeeping genes (16S rRNA, ITS1, gyrB, hsp65, recA, rpoB, and sodA). No individual phylogenetic tree allowed good discrimination of all of the species studied. However, a concatenated and a consensus analysis, combining the genes, allowed better discrimination of each strain to the species level, and the increase in sequence size also led to greater tree robustness. This approach is useful not only for the discrimination and identification of environmental mycobacteria but also for their molecular typing and studies of population genetics. Our results demonstrate high genetic diversity among the isolates obtained, which are probably new species of the genus.
Gomila
Margarita
M
Area Microbiologia, Departament de Biologia, Universitat de les Illes Balears, Campus UIB, Crtra. Valldemosa km 7.5, Facultad de Ciencias, 07122 Palma de Mallorca, and Servei de Microbiologia, Hospital Universitari Son Dureta, Illes Baleares, Spain.
Ramirez
Antonio
A
Lalucat
Jorge
J
D001483:Base Sequence; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D014644:Genetic Variation; D015312:Hemodialysis Solutions / Q000032:analysis*; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000379:methods*; D013183:Sputum / Q000382:microbiology
D017931:DNA Primers; D015312:Hemodialysis Solutions