Passive transfer of experimental autoimmune neuritis by IL-12 and IL-18 synergistically potentiated lymphoid cells is regulated by NKR-P1+ cells.
The aim of this study was to investigate the roles and mechanism of interleukin-12 (IL-12) and interleukin-18 (IL-18) in potentiating the autoreactivity of lymphoid cells specific for P2 53-78 peptide. P2 53-78-specific lymphoid cells in the presence of IL-12 or IL-18 alone passive transferred only moderate experimental autoimmune neuritis (EAN) into a low percentage of recipients. However, lymphoid cells co-cultured with both cytokines transferred aggressive clinical and histological EAN into all recipients. NKR-P1+ cells (including NK and NKT cells) played an immunosuppressive function in passive transfer EAN and depletion of NKR-P1+ cells by anti-NKR-P1 Ab and complement induced a more serious form of EAN. Nevertheless, lymphoid cells co-cultured with both IL-12 and IL-18 induced high levels of interferon-gamma (IFN-gamma) and promoted Th1 differentiation partially through NKR-P1+ cells and to some extent, NKR-P1+ cell depletion inhibited the auto-reactivity of lymphoid cells treated with IL-12 and IL-18.
Sun
B
B
Department of Neurobiology, Harbin Medical University Provincial Key Lab of Neurobiology, Harbin, China.
Li
H-L
HL
Wang
J-H
JH
Wang
G-Y
GY
Zhao
R
R
Mu
L-L
LL
Jin
L-H
LH
D000276:Adjuvants, Immunologic / Q000502:physiology*; D019264:Adoptive Transfer / Q000379:methods; D000818:Animals; D000954:Antigens, Surface / Q000096:biosynthesis*; D018920:Coculture Techniques; D004357:Drug Synergism; D005260:Female; D018664:Interleukin-12 / Q000502:physiology*; D020382:Interleukin-18 / Q000502:physiology*; D007694:Killer Cells, Natural / Q000276:immunology / Q000378:metabolism / Q000637:transplantation*; D037181:Lectins, C-Type / Q000096:biosynthesis*; D008212:Lymphocyte Depletion; D017710:Lymphocyte Transfusion; D051379:Mice; D055651:NK Cell Lectin-Like Receptor Subfamily B; D009444:Neuritis, Autoimmune, Experimental / Q000276:immunology* / Q000473:pathology; D051381:Rats; D011917:Rats, Inbred Lew; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism / Q000637:transplantation*
D000276:Adjuvants, Immunologic; D000954:Antigens, Surface; D020382:Interleukin-18; D037181:Lectins, C-Type; D055651:NK Cell Lectin-Like Receptor Subfamily B; D018664:Interleukin-12