Microarray-based molecular detection of foot-and-mouth disease, vesicular stomatitis and swine vesicular disease viruses, using padlock probes.
The World Organization for Animal Health (Office International des Epizooties, OIE) includes the diseases caused by foot-and-mouth disease virus (FMDV), swine vesicular disease virus (SVDV), and vesicular stomatitis virus (VSV), as "Diseases Notifiable to the OIE". Foot-and-mouth disease (FMD) outbreaks have severe economical as well as social effects and cannot be differentiated from the diseases caused by the other two viruses on the basis of clinical symptoms. Efficient laboratory techniques are therefore required for detection and identification of the viruses causing similar vesicular symptoms in swine. A rapid method is described using padlock probes and microarrays to detect simultaneously and differentiate the three viruses in a single reaction, as well as providing serotype information in cases of VSV infection. The padlock probe/microarray assay detected successfully and identified 39 cDNA samples of different origin representing the three viruses. The results were in complete agreement with identities and serotypes determined previously. This novel virus detection method is discussed in terms of usefulness and further development.
Banér
Johan
J
Joint Research and Development Division, Department of Virology, The National Veterinary Institute and the Swedish University of Agricultural Sciences, Ulls väg 2B, SE-75189 Uppsala, Sweden.
Gyarmati
Péter
P
Yacoub
Alia
A
Hakhverdyan
Mikhayil
M
Stenberg
Johan
J
Ericsson
Olle
O
Nilsson
Mats
M
Landegren
Ulf
U
Belák
Sándor
S
D000818:Animals; D001483:Base Sequence; D015342:DNA Probes; D029822:Enterovirus B, Human / Q000235:genetics / Q000302:isolation & purification*; D005536:Foot-and-Mouth Disease / Q000175:diagnosis*; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes; D018353:Rhabdoviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*; D013555:Swine Vesicular Disease / Q000175:diagnosis*; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics / Q000302:isolation & purification*
D015342:DNA Probes; D015345:Oligonucleotide Probes