title: Transcriptional profile of Rous Sarcoma Virus transformed chicken embryo fibroblasts reveals new signaling targets of viral-src.
abstract: Transformation of chicken fibroblasts in vitro by Rous Sarcoma Virus represents a model of cancer in which a single oncogene, viral src, uniformly and rapidly transforms primary cells in culture. We experimentally surveyed the transcriptional program affected by Rous Sarcoma Virus (RSV) in primary culture of chicken embryo fibroblasts. As a control, we used cells infected with non-transforming RSV mutant td106, in which the src gene was deleted. Using Affymetrix GeneChip Chicken Genome Arrays, we report 811 genes that were modulated more than 2.5 fold in the virus transformed cells. Among these, 409 genes were induced and 402 genes were repressed by viral src. From the repertoire of modulated genes, we selected 20 genes that were robustly changed. We then validated and quantified the transcriptional changes of most of the 20 selected genes by real-time PCR. The set of strongly induced genes contains vasoactive intestinal polypeptide, MAP kinase phosphatase 2 and follistatin, among others. The set of strongly repressed genes contains TGF beta 3, TGF beta-induced gene, and deiodinase. The function of several robustly modulated genes sheds new light on the molecular mechanism of oncogenic transformation.
authors: Weis Center for Research, Danville, PA 17822-2608, USA.
authors: Kathryn
authors: K
authors: Masker
authors: Alicia
authors: A
authors: Golden
authors: Christian J
authors: CJ
authors: Gaffney
authors: Virginia
authors: V
authors: Mazack
authors: William F
authors: WF
authors: Schwindinger
authors: Weizhou
authors: W
authors: Zhang
authors: Lu-Hai
authors: LH
authors: Wang
authors: David J
authors: DJ
authors: Carey
authors: Marius
authors: M
authors: Sudol
mesh_terms: D000818:Animals; D001483:Base Sequence; D002472:Cell Transformation, Viral / Q000235:genetics; D002478:Cells, Cultured; D002642:Chick Embryo; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D020869:Gene Expression Profiling; D005814:Genes, Viral; D016391:Genes, src; D008957:Models, Genetic; D020411:Oligonucleotide Array Sequence Analysis; D052736:Rous sarcoma virus / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D015398:Signal Transduction; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D004279:DNA, Viral