Increased inflammatory factors activity in model rats with experimental autoimmune prostatitis.
Male rats were immunized with prostate tissue homogenate supernate (PTHS) of male rats with complete Freund's adjuvant (CFA) intra dermal in the multiple points and simultaneously immunized with 0.5 ml Pertussis-Diphtheria-Tetanus (PDT) vaccine intra peritonea on 0 and 30th day. At the 45th day after first immunization, animals were sacrificed and a series of examinations such as HE stain, assay of TNF-alpha by ELISA and assay of inducible nitric oxide synthase (iNOS) mRNA by in-situ hybridization (ISH) were taken. We observed that there was a remarkable up-regulation of TNF-alpha expression in the high dosage model group. The results of macropathology, histopathology and iNOS ISH also revealed the same tendency. This experimental procedure is effective to induce chronic abacterial prostatitis (CAP).
Zhou
Xiao-hui
XH
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang province, PRC.
Li
Lian-da
LD
Wu
Li-mao
LM
Han
Lei
L
Liu
Zhong-de
ZD
Yang
Ji-xiang
JX
Lv
Yan-wei
YW
You
Chun-lai
CL
Zhou
Zhi-heng
ZH
D000818:Animals; D001327:Autoimmune Diseases / Q000473:pathology / Q000503:physiopathology*; D004195:Disease Models, Animal; D017403:In Situ Hybridization; D007249:Inflammation / Q000503:physiopathology*; D008297:Male; D052247:Nitric Oxide Synthase Type II / Q000235:genetics; D011472:Prostatitis / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D014409:Tumor Necrosis Factor-alpha; D052247:Nitric Oxide Synthase Type II