[Function of the putative Na+/H+ antiporter gene PeNhaD1 from salt-resistant Populus euphratica Oliv].
Yeast complementation experiments were carried out to define the possible function of PeNhaD1, a Na(+)/H(+) antiporter gene from Populus euphratica Oliv., a salt resistant tree. PeNhaD1 was introduced to the Saccharomyces cerevisiae mutant strain ANT3 (Deltaena1-4::HIS3 Deltanha1::LEU2), which lacks the plasma membrane Na(+)/H(+) antiporter gene ScNHA1 or GX1 (Deltanhx1::TRP1), which lacks tonoplast Na(+)/H(+) antiporter gene ScNHX1. Our results showed that PeNhaD1 rescued the normal growth of ANT3 in the presence of high salt (80 mmol/L NaCl on solid medium or 400 mmol/L in liquid medium, pH 6.0), but not that of GX1, suggesting that PeNhaD1 may play a role in salt tolerance of Populus euphratica by maintaining the capacity for salt exclusion under saline condition.
Lv
Ping-Ping
PP
College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
Hu
Jun
J
Chen
Shao-Liang
SL
Shen
Xin
X
Yin
Wei-Bo
WB
Chen
Yu-Hong
YH
Sun
Yong-Ru
YR
Hu
Zan-Min
ZM
D015870:Gene Expression / Q000187:drug effects; D005816:Genetic Complementation Test; D009154:Mutation; D010940:Plant Proteins / Q000235:genetics / Q000502:physiology*; D032107:Populus / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D012965:Sodium Chloride / Q000494:pharmacology*; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics / Q000502:physiology*
D010940:Plant Proteins; D017923:Sodium-Hydrogen Exchangers; D012965:Sodium Chloride