Effects of light on the feedback control of GA-20 oxidase gene homolog in DongJinByeo seedlings.
The effects of gibberellin (GA) on the expression of GA-20 oxidase gene homolog were examined in light-grown seedlings and dark-grown seedlings of DongJinByeo. The growth rates of the stems of etiolated seedlings were faster than those of green seedlings. However, upon addition of GA to these seedlings, the stem growth rates of green seedlings were faster than those of etiolated seedlings. To understand the molecular mechanism of GA gene regulation in DongJinByeo, total RNA from DongJinByeo was hybridized with cDNA of GA-20 oxidase gene homolog. Greater accumulation of transcript of GA-20 oxidase gene homolog was observed in green seedlings than in etiolated seedlings. However, upon addition of GA, higher accumulation of the gene transcript was found in etiolated seedlings than in green seedlings, indicating that expression of the transcript of GA-20 oxidase gene homolog might be inhibited by light. These results suggest that light might regulate feedback control of the transcript of GA-20 oxidase gene homolog in DongJinByeo.
Kim
JaeHong
J
Division of Life Science, Soonchunhyang University, Chungnam, 336-600, Korea.
Yoon
InSun
I
Lee
MiYoung
M
D001483:Base Sequence; D017931:DNA Primers; D005246:Feedback / Q000528:radiation effects*; D008027:Light; D006899:Mixed Function Oxygenases / Q000235:genetics*; D012275:Oryza / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects*; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D006899:Mixed Function Oxygenases; C089210:gibberellin, 2-oxoglutarate-oxygen oxidoreductase (20-hydroxylating, oxidizing)