Molecular characteristics and expression analysis of calreticulin in Chinese shrimp Fenneropenaeus chinensis.
Calreticulin (CRT), as an endoplasmic reticulum luminal resident protein, plays important roles in Ca(2+) homeostasis and molecular chaperoning. CRT on the surface of the cell can modulate cell adhesion, phagocytosis and integrin-dependent Ca(2+) signaling. The full length cDNA of calreticulin (FcCRT) was cloned from Chinese shrimp Fenneropenaeus chinensis. It consists of 1672 bp with an open reading frame of 1221 bp, encoding 406 amino acids. This is the first reported cDNA sequence of calreticulin in Crustacea. The deduced amino acid sequence of FcCRT showed high identity with those of Bombyx mori (88%), Drosophila melanogaster (83%), Mus musculus (82%) and Homo sapiens (82%). Highest expression of FcCRT was detected in ovary by Northern blot and in situ hybridization. Different mRNA levels of FcCRT were detected at various molting stages. Expression of FcCRT was induced significantly after 3 h of heat shock treatment, reached the maximum at 4 h and dropped after that. Differential expression profiles of FcCRT were observed in hepatopancreas and haemocytes when shrimp were challenged by white spot syndrome virus (WSSV). From the above results, we inferred that FcCRT might play important roles in Ca(2+) homeostasis, chaperoning and immune function in shrimp.
Luana
Wei
W
Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, PR China; Graduate School, Chinese Academy of Sciences, Beijing 100039, PR China.
Li
Fuhua
F
Wang
Bing
B
Zhang
Xiaojun
X
Liu
Yichen
Y
Xiang
Jianhai
J
D000818:Animals; D001483:Base Sequence; D012831:Bombyx; D002118:Calcium / Q000378:metabolism; D037282:Calreticulin / Q000096:biosynthesis* / Q000235:genetics; D004331:Drosophila melanogaster; D005786:Gene Expression Regulation / Q000502:physiology*; D018869:Heat-Shock Response / Q000502:physiology; D006434:Hemocytes / Q000378:metabolism; D006801:Humans; D051379:Mice; D018832:Molecular Chaperones / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D018978:Molting / Q000502:physiology; D033561:Penaeidae / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D046848:White spot syndrome virus 1
D037282:Calreticulin; D018832:Molecular Chaperones; D002118:Calcium