title: Donor B cells in splenic follicles of experimental pulmonary allograft recipients.
abstract: BACKGROUND
  After transplantation, passenger leukocytes move to lymphoid organs of the recipient. These cells appear to initiate allograft rejection, but they also might be involved in tolerance induction.
  
  
  METHODS
  Orthotopic left lung transplantation was performed in the Dark Agouti to Lewis rat strain combination with no immunosuppression. Recipient spleens were removed at intervals of 24 h until day 6 after transplantation. For comparison, spleens from renal allograft recipients were analysed. Donor-derived major histocompatibility complex (MHC) class II antigens were detected by monoclonal antibody OX76. In double-staining experiments with antibodies specific for leukocyte subpopulations, their localisation and identity was analysed.
  
  
  RESULTS
  OX76-positive leukocytes were already detected in recipient spleens on day 1 post-transplantation. They increased in number until day 3 and decreased in number thereafter. Most of them were localised in splenic follicles and expressed the B cell variant of CD45R and IgG. Cell surface antigens typical for other leukocyte subpopulations were not detected. In the spleens of renal allograft recipients, only few donor-derived cells were seen.
  
  
  CONCLUSIONS
  After lung transplantation, numerous MHC class II-positive B cells migrate to the splenic follicles of the recipient. These cells might, in part, be responsible for immunologic differences observed between renal and pulmonary allografts.
authors: Laboratory of Experimental Surgery, Department of General and Thoracic Surgery, University of Giessen Lung Center, Justus-Liebig-University Giessen, Rudolf Buchheim Str. 7, 35385, Giessen, Germany. Veronika.Grau@chiru.med.uni-giessen.de
authors: Veronika
authors: V
authors: Grau
authors: Gabriele
authors: G
authors: Fuchs-Moll
authors: Gabriela
authors: G
authors: Krasteva
authors: Markus
authors: M
authors: Hirschburger
authors: Birte
authors: B
authors: Steiniger
authors: Winfried
authors: W
authors: Padberg
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000473:pathology; D054441:Cell Migration Assays, Leukocyte; D006084:Graft Rejection / Q000276:immunology* / Q000473:pathology; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D016030:Kidney Transplantation / Q000276:immunology / Q000473:pathology; D017493:Leukocyte Common Antigens; D016040:Lung Transplantation / Q000276:immunology* / Q000473:pathology; D018655:Lymphocyte Count; D008297:Male; D011379:Prognosis; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000276:immunology* / Q000473:pathology; D014019:Tissue Donors; D014184:Transplantation, Homologous
chemical_list: D000949:Histocompatibility Antigens Class II; D017493:Leukocyte Common Antigens