[Study on CTP production from CMP by beer yeast cell immobilized in PVA].
With PVA as the carrier, the frozen beer yeast cells were immobilized for production of CTP from CMP. we explored the optimal condition of the immobilization from the aspects of the type, concentration of the PVA, and the immobilizing methods of cells In all 8 continuous batch of fermentation under the reactional condition of the immobilized cells, the conversion rate of CTP were maintained about 85% - 95%. Moreever, the storage stability of immobilized cells were investigated, and the products was also isolated and identifided by HPLC.
Yang
Hong-Yi
HY
China Graduate School of the Chinese Academy of Sciences, Beijing 100039, China. yhongyi@sina.com
Qian
Shi-Jun
SJ
Li
Gao-Wo
GW
D001515:Beer / Q000382:microbiology*; D019149:Bioreactors / Q000382:microbiology; D018914:Cells, Immobilized / Q000166:cytology / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003568:Cytidine Monophosphate / Q000378:metabolism*; D003570:Cytidine Triphosphate / Q000378:metabolism*; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods; D011142:Polyvinyl Alcohol; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism*
D003570:Cytidine Triphosphate; D011142:Polyvinyl Alcohol; D003568:Cytidine Monophosphate