title: In vivo antibacterial efficacy of MONOCRYL plus antibacterial suture (Poliglecaprone 25 with triclosan).
abstract: BACKGROUND
  This study evaluated the ability of poliglecaprone 25 suture with triclosan to inhibit bacterial colonization by Escherichia coli and Staphylococcus aureus in mouse and guinea pig models.
  
  
  METHODS
  Test and control sutures (3-4 cm) were implanted subcutaneously in the dorsal-lateral regions (control on the left side, test on the right side, approximately 3-5 cm apart) in 10 female Hartley guinea pigs (300-400 g) and 10 Swiss Webster mice (20-35 g) via a 20-gauge catheter. The test material was poliglecaprone 25 suture with triclosan (2-0, undyed), and the control material was poliglecaprone 25 suture (2-0, undyed). In the guinea pig model, each implantation site was challenged directly with 4x10(5) colony-forming units (cfu) of S. aureus, whereas in the mouse model, each implantation site was challenged directly with 1.3x10(7) cfu of E. coli through an indwelling catheter. At 48 h post-implantation, the control and test sutures were explanted, and bacterial enumeration was performed.
  
  
  RESULTS
  There was a significant difference (p < 0.05) in the number of bacteria recovered in the study groups 48 h post-implantation. Poliglecaprone 25 suture with triclosan produced a 3.4-log reduction in S. aureus and a 2-log reduction in E. coli compared with standard poliglecaprone 25 suture without triclosan under the same challenge conditions. The difference between the study groups in the number of bacteria recovered was significant (p < 0.05).
  
  
  CONCLUSIONS
  Poliglecaprone 25 suture with triclosan inhibited bacterial colonization of the suture compared with untreated suture after direct in vivo challenge with S. aureus and E. coli in animal models.
authors: Corporate Microbiology and Sterilization Sciences, Ethicon Inc, Somerville, NJ 08876, USA. xming@ethus.jnj.com
authors: Xintian
authors: X
authors: Ming
authors: Michele
authors: M
authors: Nichols
authors: Stephen
authors: S
authors: Rothenburger
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D020099:Coated Materials, Biocompatible; D015169:Colony Count, Microbial; D004146:Dioxanes; D004926:Escherichia coli / Q000187:drug effects; D005260:Female; D006168:Guinea Pigs; D051379:Mice; D008826:Microbial Sensitivity Tests / Q000379:methods; D011091:Polyesters; D013211:Staphylococcus aureus / Q000187:drug effects; D013530:Surgical Wound Infection / Q000517:prevention & control*; D013537:Sutures / Q000382:microbiology*; D014260:Triclosan / Q000494:pharmacology*
chemical_list: D000891:Anti-Infective Agents, Local; D020099:Coated Materials, Biocompatible; D004146:Dioxanes; D011091:Polyesters; C095495:glycolide E-caprolactone copolymer; D014260:Triclosan