title: Statistical optimization of culture conditions for 1,3-propanediol by Klebsiella pneumoniae AC 15 via central composite design.
abstract: A central composite design was used to study the effect of glycerol, rate of stirring, air aeration and pH on the synthesis of 1,3-propanediol (1,3-PD) by Klebsiella pneumoniae AC 15. Among the four variables, glycerol and rate of stirring significantly affected 1,3-PD productivity, whereas air aeration and pH were not effective. A quadratic polynomial equation was obtained for 1,3-PD productivity by multiple regression analysis using response surface methodology. The validation experimental confirmed with the predicted model. The optimum combinations for 1,3-PD productivity was glycerol, rate of stirring, air aeration, and pH of 50 g/l, 318 rpm, 0.6 vvm, 6.48, respectively. The subsequent fed batch experiments produced 1,3-PD of 70 g/l at a fermentation of 30 h.
authors: Department of Chemistry Engineering, Tsinghua University, 100084 Beijing, China.
authors: Zong-ming
authors: ZM
authors: Zheng
authors: Qiu-long
authors: QL
authors: Hu
authors: Jian
authors: J
authors: Hao
authors: Feng
authors: F
authors: Xu
authors: Ni-ni
authors: NN
authors: Guo
authors: Yan
authors: Y
authors: Sun
authors: De-hua
authors: DH
authors: Liu
mesh_terms: D000388:Air; D001431:Bacteriological Techniques; D019149:Bioreactors / Q000382:microbiology; D003470:Culture Media; D005990:Glycerol / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007711:Klebsiella pneumoniae / Q000145:classification / Q000378:metabolism*; D011409:Propylene Glycols / Q000378:metabolism*; D013997:Time Factors
chemical_list: D003470:Culture Media; D011409:Propylene Glycols; C041787:1,3-propanediol; D005990:Glycerol