CFTR stabilizes ENaC at the plasma membrane.
CFTR was reported to regulate ENaC channel opening, decreasing ENaC activity in airways and increasing it in sweat ducts. We generated MDCK-I cell lines stably expressing tagged alphabetagammaENaC+CFTR or ENaC alone, and developed an assay to quantify cell-surface half-life of ENaC. Surprisingly, we found that co-expressed CFTR stabilizes ENaC at the plasma membrane, suggesting that CFTR regulates ENaC stability, not just opening.
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Program in Cell Biology, The Hospital for Sick Children, and Biochemistry Department, University of Toronto, 555 University Ave, Toronto, Ontario, Canada M5G 1X8.
Jiang
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Pribanic
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Rotin
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D000818:Animals; D002460:Cell Line; D002462:Cell Membrane; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics / Q000378:metabolism*; D004285:Dogs; D053503:Epithelial Sodium Channels / Q000235:genetics / Q000378:metabolism*; D006801:Humans
D053503:Epithelial Sodium Channels; D019005:Cystic Fibrosis Transmembrane Conductance Regulator