title: SPF-5506-A4, a new peptaibol inhibitor of amyloid beta-peptide formation produced by Trichoderma sp.
abstract: A new peptaibol compound, SPF-5506-A4, was isolated from the fermentation broth of Trichoderma sp. SPF-5506. The chemical structure of the 14-residue peptide was determined by MS, NMR and amino acid sequence analyses. The absolute configuration of amino acid residues in the acid hydrolysate was determined by Marfey's method. The structure of SPF-5506-A4 was established as Ac-Aib-L-Asn-L-Ile-Aib-L-Pro-L-Ser-L-Ile-Aib-L-Pro-L-Leu-L-Leu-Aib-L-Pro-L-leucinol. The compound inhibited amyloid beta-peptide formation in primary guinea pig cerebral cortex neuron cell culture dose-dependently with an IC50 of 0.1 microg/ml. Cytotoxicity was not observed at concentrations of <3 microg/ml.
authors: Exploratory Research Group, Research Division, Sumitomo Pharmaceuticals Co., Ltd, Takarazuka, Hyogo, Japan.
authors: Nobuo
authors: N
authors: Hosotani
authors: Kazuo
authors: K
authors: Kumagai
authors: Shigeyuki
authors: S
authors: Honda
authors: Akira
authors: A
authors: Ito
authors: Takuro
authors: T
authors: Shimatani
authors: Ikutaro
authors: I
authors: Saji
mesh_terms: D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000096:biosynthesis* / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005285:Fermentation; D006168:Guinea Pigs; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D009842:Oligopeptides / Q000096:biosynthesis / Q000494:pharmacology*; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D014242:Trichoderma / Q000145:classification / Q000254:growth & development / Q000378:metabolism*
chemical_list: D016229:Amyloid beta-Peptides; D009842:Oligopeptides; D010455:Peptides; C520915:SPF-5506-A4