title: Effect of the physicochemical properties of poly(ethylene glycol) brushes on their binding to cells.
abstract: We investigated the effect of the number of oxyethylene groups (polymer molecular weight) and the interchain binding and/or entanglements of methoxy-terminated-poly(ethylene glycol) (m-PEG) brushes on their ability to adsorb to living malignant melanoma B16F10 cells. We used the atomic force microscope colloid probe method to determine the adhering ability of the m-PEG brushes to the cells, as the magnitude of the adhesion force between the m-PEG modified particles and the living cells in a physiological buffer was related to the binding strength of the m-PEGs to the cells. We saw that m-PEG brushes (average molecular weights 330, 1900, and 5000 g/mol), which were chemically attached to silica particles, may bind to living B16F10 cells. The binding of m-PEGs to living B16F10 cells increased as the oxyethylene chain length of the m-PEGs increased, if the m-PEGs had a low degree of entanglements or little inter-m-PEG chain binding. A high degree of entanglements or interchain binding decreased the ability of an m-PEG chain to bind to a living cell. The effect of m-PEG (molecular weight 1900 g/mol) being present at cell surfaces for 24 h was also seen not to induce the death of the cells or affect their growth.
authors: Department of Chemical Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto, Japan. cathymcn@z06cheme.mbox.media.kyoto-u.ac.jp
authors: Cathy E
authors: CE
authors: McNamee
authors: Shinpei
authors: S
authors: Yamamoto
authors: Ko
authors: K
authors: Higashitani
mesh_terms: D000327:Adsorption; D000818:Animals; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects*; D051379:Mice; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000494:pharmacology*; D012878:Skin Neoplasms / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology*
chemical_list: D011092:Polyethylene Glycols