Diminished intestinal colonization by Clostridium difficile and immune response in mice after mucosal immunization with surface proteins of Clostridium difficile.
Clostridium difficile pathogenesis is mainly due to toxins A and B. However, the first step of pathogenesis is the colonization process. We evaluated C. difficile surface proteins as vaccine antigens to diminish intestinal colonization in a human flora-associated mouse model. First, we used the flagellar cap protein FliD of C. difficile, in order to test several immunization routes: intranasal, rectal, and intragastric. The rectal route, which is the most efficient, was used to vaccine groups of mice with different antigen combinations. After immunizations, the mice were challenged with the toxigenic C. difficile and a significant statistical difference between the control group and the immunized groups was observed in the colonization levels of C. difficile.
Péchiné
Séverine
S
Université de Paris-Sud, Faculté de Pharmacie, EA 4043, USC INRA, 5 rue JB Clement, F-92296 Châtenay-Malabry cedex, France. severine.pechine@u-psud.fr
Janoir
Claire
C
Boureau
Hélène
H
Gleizes
Aude
A
Tsapis
Nicolas
N
Hoys
Sandra
S
Fattal
Elias
E
Collignon
Anne
A
D000285:Administration, Rectal; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000276:immunology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000276:immunology; D002772:Cholera Toxin / Q000276:immunology / Q000494:pharmacology; D016360:Clostridioides difficile / Q000254:growth & development* / Q000276:immunology; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000276:immunology; D004761:Enterocolitis, Pseudomembranous / Q000276:immunology* / Q000382:microbiology*; D005260:Female; D006801:Humans; D018928:Immunity, Mucosal / Q000276:immunology; D007070:Immunoglobulin A / Q000096:biosynthesis / Q000276:immunology; D007422:Intestines / Q000276:immunology / Q000382:microbiology*; D051379:Mice; D008809:Mice, Inbred C3H
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D007070:Immunoglobulin A; C076733:FlaD protein, Bacteria; D002772:Cholera Toxin; D003546:Cysteine Endopeptidases