title: Intralaboratory validation according to the EN ISO 16 140 Standard of the Vidas SET2 detection kit for use in official controls of staphylococcal enterotoxins in milk products.
abstract: OBJECTIVE
  Immunological tools used to detect staphylococcal enterotoxins (SEs) in foods are numerous. The aim of this study was to evaluate, on naturally contaminated milk product samples, the performance of the Vidas SET2, in comparison to the Transia plate SET.
  
  
  RESULTS
  The Vidas SET2 was compared with the Transia plate SET on supernatants of Staphylococcus aureus isolates and on naturally contaminated milk products. It is noteworthy that when using IgG rabbit treatment, both kits can be considered as equivalent to detect enterotoxins in naturally contaminated milk products.
  
  
  CONCLUSIONS
  This study demonstrated that the Vidas SET2 performance is similar to that of Transia plate SET kit, when a rabbit IgG treatment step is used before detection step. This additional treatment significantly decreased, from 42% to 8%, the rate of positive deviations observed using the Transia plate SET detection kit.
  
  
  CONCLUSIONS
  The Vidas SET2 was clearly found as more specific, when no preliminary rabbit IgG treatment was used, and which results in a better workflow when a large number of samples have to be analysed within a few days. Considering the results obtained, the Vidas SET2 detection kit can be used to assess the safety of milk products for SEs.
authors: Laboratory for Study and Research on Quality of Food and on Food Processes, French Agency for Food Safety (AFSSA-lerqap), National and Community Reference Laboratory, Maisons-Alfort cedex, France. ja.hennekinne@afssa.fr
authors: J-A
authors: JA
authors: Hennekinne
authors: F
authors: F
authors: Guillier
authors: S
authors: S
authors: Perelle
authors: M-L
authors: ML
authors: De Buyser
authors: S
authors: S
authors: Dragacci
authors: S
authors: S
authors: Krys
authors: B
authors: B
authors: Lombard
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002623:Chemistry Techniques, Analytical / Q000379:methods; D003611:Dairy Products / Q000382:microbiology*; D004768:Enterotoxins / Q000032:analysis*; D005504:Food Analysis / Q000379:methods / Q000592:standards*; D005516:Food Microbiology; D008892:Milk / Q000382:microbiology*; D011817:Rabbits; D013211:Staphylococcus aureus / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D004768:Enterotoxins