[Experimental study of Qishen Yiqi Dropping Pills on liver fibrosis in rats].
OBJECTIVE
To investigate the anti-fibrotic effects of Qishen Yiqi Dropping Pills (QYDP) in rats with liver fibrosis (LF).


METHODS
The LF model was induced by intraperitoneal injection with dimethylnitrosamine (DMN). Sixty Wistar rats were randomly divided into the normal group, the model group A, the QYDP intervened group , the model group B , and the QYDP treated group B. The degree of LF was evaluated according to 6-phase grading method. The expressions of collagen type I and III and tissue inhibitor of metalloproteinase-1 (TIMP-1) in liver tissues were determined by immunohistochemistry and the levels of collagen type I and III and TIMP-1 mRNA determined by semi-quantitive RT-PCR.


RESULTS
Compared with the model group A and B, the degree of LF, the positive expressions of TIMP-1 mRNA and the content of collagen type I and III in liver tissue in the QYDP intervened and treated groups were significantly lower.


CONCLUSIONS
QYDP could reduce the pathological changes and degree of LF in rats, which may be partially through inhibiting the expressions of collagen type I and III and TIMP-1.
Zhu
Yue-ke
YK
Artificial Liver Center, Beijing You'an Hospital, Capital University of Medical Sciences. zhuyk126@126.com
Duan
Zhong-ping
ZP
Wang
Bao-en
BE
D000818:Animals; D024042:Collagen Type I / Q000096:biosynthesis* / Q000235:genetics; D024061:Collagen Type III / Q000096:biosynthesis / Q000235:genetics; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D007150:Immunohistochemistry; D008106:Liver Cirrhosis, Experimental / Q000188:drug therapy*; D008297:Male; D008517:Phytotherapy; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000096:biosynthesis* / Q000235:genetics
D024042:Collagen Type I; D024061:Collagen Type III; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D019715:Tissue Inhibitor of Metalloproteinase-1; C528580:qishen yiqi