Diagnostic markers in the structures of human biological liquids.
BACKGROUND
To date, morphology research in laboratory diagnostics has referred only to cell tissues and excluded biological fluids. Our new technology is based on the study of structures of biological fluids formed during transition from liquid to solid state by dehydration under special conditions. These structures are formed due to the special "mosaic" configuration of molecules and microaggregates of the substance (protein and others) dissolved in the studied fluids. It should be considered as well that the specific structure of protein molecules is a fundamental basis of anatomical and physiological peculiarities of an organism. All pathological processes are initiated by the changes in the spatial configuration of protein molecules.


METHODS
We have developed a special method named "cuneiform dehydration of biological liquids" to obtain thin films which possess specific structures. During the transition of a biological liquid into a solid, organic molecules build specific macrostructures in conformity with their configuration.


RESULTS
Studies of the solid phase structures of different biological liquids allow us to find specific markers of various pathological processes in the human organism. In blood serum, we discovered markers of inflammation, sclerosis, chronic intoxication; in tear--markers of different stages of glaucoma; and in synovial fluid--markers of different stages of osteoarthrosis. In our investigation of 143 healthy people and 1,419 patients, a high rate of compliance of the discovered markers with the results of routine clinical and laboratory methods were observed. As the suggested technology has a higher sensitivity, the results obtained by this technology and routine methods do not always coincide.


CONCLUSIONS
The developed technology gives new information about the state of the human organism and may be used in practice for diagnostics of various pathological processes and at pre-clinical studies of their development, as well as for the evaluation of the efficacy of medical treatment.
Shabalin
V N
VN
Russian Scientific Research Institute of Gerontology, Ministry of Health, Moscow, Russia. du00321@postman.ru
Shatokhina
S N
SN
D015415:Biomarkers / Q000032:analysis*; D001826:Body Fluids / Q000737:chemistry*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003890:Desiccation; D006801:Humans; D044367:Phase Transition
D015415:Biomarkers