Design and switch of catalytic activity with the DNAzyme-RNAzyme combination.
Design and switch of catalytic activity in enzymology remains a subject of intense investigation. Here, we employ a DNAzyme-RNAzyme combination strategy for construction of a 10-23 deoxyribozyme-hammerhead ribozyme combination that targets different sites of the beta-lactamase mRNA. The 10-23 deoxyribozyme-hammerhead ribozyme combination gene was cloned into phagemid vector pBlue-scriptIIKS (+). In vitro the single-strand recombinant phagemid vector containing the combination sequence exhibited 10-23 deoxyribozyme activity, and the linear transcript displayed hammerhead ribozyme activity. In bacteria, the 10-23 deoxyribozyme-hammerhead ribozyme combination inhibited the beta-lactamase expression and repressed the growth of drug-resistant bacteria. Thus, we created a DNAzyme-RNAzyme combination strategy that provides a useful approach to design and switch of catalytic activities for nucleic acid enzymes.
Sheng
Yongjie
Y
Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun 130023, PR China.
Zeng
Zhen
Z
Peng
Wei
W
Jiang
Dazhi
D
Li
Shuang
S
Sun
Yanhong
Y
Zhang
Jin
J
D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002384:Catalysis; D002413:Cations, Divalent / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods*; D021881:DNA, Catalytic / Q000138:chemical synthesis* / Q000378:metabolism; D004274:DNA, Recombinant / Q000138:chemical synthesis; D008954:Models, Biological; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016337:RNA, Catalytic / Q000138:chemical synthesis* / Q000378:metabolism; D001618:beta-Lactamases / Q000378:metabolism
D002413:Cations, Divalent; D021881:DNA, Catalytic; D004274:DNA, Recombinant; D016337:RNA, Catalytic; C427204:hammerhead ribozyme; D001618:beta-Lactamases