Live imaging of spindle pole disorganization in docetaxel-treated multicolor cells.
Treatment of cells with docetaxel at low concentrations induces aberrant bipolar spindles of which two centrosomes stay at only one pole, and also induces multipolar spindles. To gain insight into the relations between centrosome impairment and structural defects of the spindle, live-cell imaging was performed on a human MDA Auro/imp/H3 cell line in which centrosomes/mitotic spindles, nuclear membrane and chromatin were simultaneously visualized by fluorescent proteins. In the presence of docetaxel at IC(50) concentration, the centrosomes did not segregate, and multiple aster-like structures ectopically arose around the disappearing nuclear membrane. Those ectopic structures formed an acentrosomal pole opposing to the two-centrosomes-containing pole. In late metaphase, one pole often fragmented into multiple spindle poles, leading multipolar division. These results suggest that spindle pole fragility may be induced by centrosome impairment, and collapse of the pole may contribute to induction of aneuploid daughter cells.
Sakaushi
Shinji
S
Laboratory of Molecular Biology and Cell Informatics, Division of Bioscience and Informatics, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan. ssaka@biochem.osakafu-u.ac.jp
Nishida
Kumi
K
Minamikawa
Harumi
H
Fukada
Takashi
T
Oka
Shigenori
S
Sugimoto
Kenji
K
D000970:Antineoplastic Agents / Q000494:pharmacology*; D064127:Aurora Kinases; D002455:Cell Division; D045744:Cell Line, Tumor; D018385:Centrosome / Q000187:drug effects* / Q000502:physiology; D000077143:Docetaxel; D004305:Dose-Response Relationship, Drug; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007399:Interphase; D008856:Microscopy, Fluorescence / Q000379:methods*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D008941:Spindle Apparatus / Q000187:drug effects* / Q000502:physiology; D043823:Taxoids / Q000494:pharmacology*; D013997:Time Factors
D000970:Antineoplastic Agents; D043823:Taxoids; D049452:Green Fluorescent Proteins; D000077143:Docetaxel; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases