Gene expression analysis on the early development of pig embryos exposed to malathion.
Malathion is a widely used pesticide and there is evidence that it could alter mammal's germ and somatic cells, as well as cell lines. There are not enough studies showing how the nonacute malathion doses affect gene expression. This study analyzes gene expression alterations in pig morular embryos exposed in vitro, for 96 h, to several malathion concentrations after in vitro fertilization. cDNA libraries of isolated morular embryos were created and differential screenings performed to identify target genes. Seven clones were certainly identified. Genes related to mitochondrial metabolism as cytochrome c subunits I and III, nuclear genes such as major histocompatibility complex I (MHC I), and a hypothetical protein related with a splicing factor were the target of malathion's deregulation effect. The widespread use of malathion as a pesticide should be regarded with reproductive implications and more detailed analysis would yield more about molecular mechanisms of malathion injury on embryo cells.
Salazar
Zayil
Z
Doctorado en Biología Experimental, CBS, Universidad Autónoma Metropolitana, Iztapalapa, México, D. F. México.
Ducolomb
Yvonne
Y
Betancourt
Miguel
M
Bonilla
Edmundo
E
Cortés
Leticia
L
Hernández-Hernández
Fidel
F
González-Márquez
Humberto
H
D000818:Animals; D004305:Dose-Response Relationship, Drug; D046149:Embryo Culture Techniques; D020964:Embryo Loss / Q000139:chemically induced; D004622:Embryo, Mammalian / Q000187:drug effects* / Q000196:embryology / Q000378:metabolism; D047108:Embryonic Development / Q000187:drug effects* / Q000235:genetics; D005260:Female; D005307:Fertilization in Vitro; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D015723:Gene Library; D007306:Insecticides / Q000633:toxicity*; D008294:Malathion / Q000633:toxicity*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D013552:Swine
D007306:Insecticides; D008294:Malathion