The effect of basketball training on the players' erythrocyte membrane acetylcholinesterase, (Na+,K+)-ATPase and Mg2+-ATPase activities.
The aim of this study was to investigate whether the activities of erythrocyte membrane acetylcholinesterase (AChE), (Na (+),K (+))-ATPase and Mg (2+)-ATPase are modulated by a basketball training. Blood was obtained from 10 basketball players pre- and postexercise. Total antioxidant status (TAS), lactate and pyruvate concentrations were determined with kits, while the enzyme activities were determined spectrophotometrically. Post-training blood lactate and pyruvate concentrations as well as AChE (2.90 +/- 0.05 vs. 3.98 +/- 0.09 Delta OD/min . mg protein, p < 0.01) and Na (+),K (+)-ATPase (0.58 +/- 0.04 vs. 1.27 +/- 0.12 micromol Pi/h . mg protein, p < 0.001) activities were remarkably increased, whereas TAS was significantly decreased. Mg (2+)-ATPase activity remained unaltered at the end of the training. In conclusion, the stimulation of AChE and Na (+),K (+)-ATPase by the training may be due to the rise of blood catecholamine oxidation contributing to TAS decrease and/or the increase of serotonin levels. This stress condition may modulate cholinergic and catecholaminergic/serotoninergic functions in players.
Parthimos
T
T
Department of Experimental Physiology, Medical School, University of Athens, Athens, Greece.
Tsopanakis
C
C
Angelogianni
P
P
Schulpis
K H
KH
Parthimos
N
N
Tsakiris
S
S
D000110:Acetylcholinesterase / Q000378:metabolism*; D000293:Adolescent; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism; D001490:Basketball; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000201:enzymology*; D015444:Exercise / Q000502:physiology; D006115:Greece; D006801:Humans; D008297:Male; D018384:Oxidative Stress; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D000975:Antioxidants; D000110:Acetylcholinesterase; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase