title: Lab-on-a-chip technologies for oral-based cancer screening and diagnostics: capabilities, issues, and prospects.
abstract: The design of a microfluidic lab-on-a-chip system for point-of-care cancer screening and diagnosis of oral squamous cell carcinoma (OSCC) is presented. The chip is based on determining a approximately 30-gene transcription profile in cancer cells isolated from oral fluid samples. Microfluidic cell sorting using magnetic beads functionalized with an antibody against cancer-specific cell-surface antigens (e.g., epithelial cell adhesion molecule [EpCAM]) is described. A comprehensive cancer diagnostics chip will integrate microfluidic components for cell lysis, nucleic acid extraction, and amplification and detection of a panel of mRNA isolated from a subpopulation of cancer cells contained in a clinical specimen.
authors: Department of Mechanical Engineering and Applied Science, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6315, USA.
authors: Michael G
authors: MG
authors: Mauk
authors: Barry L
authors: BL
authors: Ziober
authors: Zongyuan
authors: Z
authors: Chen
authors: Jason A
authors: JA
authors: Thompson
authors: Haim H
authors: HH
authors: Bau
mesh_terms: D000912:Antibodies, Neoplasm / Q000032:analysis; D000951:Antigens, Neoplasm / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000737:chemistry / Q000175:diagnosis* / Q000473:pathology; D005240:Feasibility Studies; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008403:Mass Screening; D046208:Microchip Analytical Procedures / Q000191:economics; D044085:Microfluidics; D009062:Mouth Neoplasms / Q000737:chemistry / Q000175:diagnosis* / Q000473:pathology; D011237:Predictive Value of Tests
chemical_list: D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm