Direct immobilization of cholesteryl-TEG-modified oligonucleotides onto hydrophobic SU-8 surfaces.
We introduce a rapid, simple one-step procedure for the high-yield immobilization of cholesteryl-tetraethyleneglycol-modified oligonucleotides (chol-DNA) at hydrophobic sites made of SU-8 photoresist. Topographic structures of SU-8 were microfabricated on microscope glass coverslips sputtered with a Ti/Au layer. Upon application, chol-DNA adsorbed to the SU-8 structures from solution, leaving the surrounding gold surface free of chol-DNA. chol-DNA immobilization is complete within 15 min and yields a surface coverage in the range of 20-95 pmol/cm(2), which corresponds to a film density of 10(12)-10(13) molecules/cm(2). chol-DNA immobilization is stable and can be sustained despite rinsing, drying, dry storage for several hours, and rehydration of chips. Furthermore, complementary DNA in solution hybridizes efficiently to immobilized chol-DNA.
Erkan
Yavuz
Y
Department of Chemistry and Bioscience, Chalmers University of Technology, Kemivägen 10, 41296 Gothenburg, Sweden.
Czolkos
Ilja
I
Jesorka
Aldo
A
Wilhelmsson
L Marcus
LM
Orwar
Owe
O
D002784:Cholesterol / Q000737:chemistry*; D005026:Ethylene Glycols / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D009841:Oligonucleotides / Q000737:chemistry*; D013499:Surface Properties
D005026:Ethylene Glycols; D009841:Oligonucleotides; D002784:Cholesterol