Mutagenic evaluation and chemical investigation of Byrsonima intermedia A. Juss. leaf extracts.
Byrsonima intermedia is a native species of the cerrado formation (tropical American savannah). In Brazil, this plant has been used for the treatment of fever, in ulcers, as a diuretic, as antiasthmatics and in skin infections. Members of the genus Byrsonima (Malpighiaceae) are employed not only in the folk medicine but also as food to make juice, jellies and liquor. The aim of this work was to evaluate the mutagenic effects of Byrsonima intermedia, common name 'murici'. Phytochemical analysis of methanol extract furnished (+)-catechin, (-)-epicatechin, quercetin-3-O-beta-d-galactopyranoside, methyl gallate, gallic acid, quercetin-3-O-alpha-l-arabinopyranoside, amentoflavone, quercetin, quercetin-3-O-(2''-O-galloyl)-beta-galactopyranoside and quercetin-3-O-(2''-O-galloyl)-alpha-arabinopyranoside. Methanol, hydromethanol and chloroform extracts were evaluated in mutagenic assay with Salmonella typhimurium (Ames test) and mice (Micronucleus test). The methanolic extract presented signs of mutagenic activity for the strains TA98 and TA100 in the Ames assay. Mutagenicity was not observed in vivo.
Sannomiya
Miriam
M
Araraquara Institute of Chemistry, São Paulo State University, Araraquara, SP, Brazil.
Cardoso
Cássia R P
CR
Figueiredo
Maria E
ME
Rodrigues
Clenilson M
CM
dos Santos
Lourdes C
LC
dos Santos
Fabio V
FV
Serpeloni
Juliana M
JM
Cólus
Ilce M S
IM
Vilegas
Wagner
W
Varanda
Eliana A
EA
D000818:Animals; D002725:Chloroform; D003520:Cyclophosphamide / Q000633:toxicity; D000432:Methanol; D051379:Mice; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D010936:Plant Extracts / Q000633:toxicity; D018515:Plant Leaves / Q000737:chemistry; D010944:Plants / Q000737:chemistry* / Q000633:toxicity*; D051381:Rats; D012156:Reticulocytes / Q000187:drug effects / Q000648:ultrastructure; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D012997:Solvents
D009153:Mutagens; D010936:Plant Extracts; D012997:Solvents; D002725:Chloroform; D003520:Cyclophosphamide; D000432:Methanol