title: A proteomic study of the major allergens from yellow jacket venoms.
abstract: The venoms of stinging insects belong to the most dangerous allergen sources and can cause fatal anaphylactic reactions. Reliable prediction of a patient's risk to anaphylactic reactions is vital, and diagnosis requires the knowledge of the relevant allergens. Recently, a new hyaluronidase -like glycoprotein from Vespula vulgaris (Ves v 2b) was identified. This led us to investigate hyaluronidases and also other major allergens from V. germanica and four additional Vespula species. By MALDI-Q-TOF-MS, the new hyaluronidase-like protein was shown to be the major component of the 43-kDa band in all Vespula species studied. LC-ESI-Q-TOF-MS/MS sequencing of Ves g 2a and Ves g 2b facilitated the cloning of their cDNA. Ves v 2b and Ves g 2b turned out to be essentially identical on protein level. Whereas the less abundant "a" form displayed enzymatic activity, the new "b" homologue did not. This is probably caused by amino acid exchanges in the active site, and it raises questions about the physiological role of this protein. Sequence comparisons by MS/MS of antigen 5 and phospholipases from V. vulgaris, germanica, maculifrons, pensylvanica, flavopilosa and squamosa revealed the latter as a taxonomic outlier and led to the discovery of several not previously reported amino acid differences.
authors: Biochemistry Division, Department of Chemistry, University of Natural Resources and Applied Life Sciences (BOKU), Vienna, Austria. daniel.kolarich@boku.ac.at
authors: Daniel
authors: D
authors: Kolarich
authors: Andreas
authors: A
authors: Loos
authors: Renaud
authors: R
authors: Léonard
authors: Lukas
authors: L
authors: Mach
authors: Gorji
authors: G
authors: Marzban
authors: Wolfgang
authors: W
authors: Hemmer
authors: Friedrich
authors: F
authors: Altmann
mesh_terms: D000485:Allergens / Q000032:analysis* / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D006801:Humans; D019476:Insect Proteins / Q000032:analysis / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D020543:Proteome / Q000032:analysis*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014862:Wasp Venoms / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D014863:Wasps
chemical_list: D000485:Allergens; D019476:Insect Proteins; D020543:Proteome; D011994:Recombinant Proteins; D014862:Wasp Venoms