The choice of conditions for cathepsin D activity determination in human saliva.
The aim of the study was the demonstration and choice of conditions for the determination of cathepsin D activity in human mixed saliva. The 6% solution of hemoglobin, denatured with hydrochloric acid, was used as the substrate. The ratio of saliva volume to hemoglobin was 4:1 w/v. The reaction was interrupted by adding 10% trichloroacetic acid, after 6 hours of incubation at 37 degrees C. The increase in degradation products was determined with the use of Folina and Ciocalteau method with copper modification.
Roszkowska-Jakimiec
W
W
Department of Instrumental Analysis, Medical University of Białystok, Poland. analist@amb.edu.pl
Dabrowska
E
E
Gacko
M
M
Karwowska
A
A
Chlabicz
M
M
D002402:Cathepsin D / Q000032:analysis*; D004796:Clinical Enzyme Tests / Q000592:standards*; D006454:Hemoglobins / Q000737:chemistry; D006801:Humans; D006851:Hydrochloric Acid / Q000737:chemistry; D008982:Molybdenum / Q000737:chemistry; D012463:Saliva / Q000737:chemistry*; D014238:Trichloroacetic Acid / Q000737:chemistry; D017973:Tungsten Compounds / Q000737:chemistry
C029556:Folin's phenol reagent; D006454:Hemoglobins; D017973:Tungsten Compounds; D014238:Trichloroacetic Acid; D008982:Molybdenum; D002402:Cathepsin D; D006851:Hydrochloric Acid