Ovsynch plus CIDR protocol for timed embryo transfer in suckled postpartum Japanese Black beef cows.
We compared synchronization and pregnancy rates, and the increase in blood progesterone concentrations during luteal development, between (1) Ovsynch plus an intravaginal controlled internal drug release (CIDR) device protocol followed by timed embryo transfer (timed ET), and (2) a conventional estrus synchronization method using PGF(2 alpha) and ET in suckled postpartum Japanese Black beef cows. Cows in the PGF group (n=18) received a PGF(2 alpha) analogue when a CL was first palpated per rectum at 10-d intervals after 1 to 2 month postpartum. Cows (n=11), which showed estrus (Day 0) within 5 d of the PGF(2 alpha), and had a CL on Day 7, received ET. Cows in the Ovsynch+CIDR group (n=19) underwent the Ovsynch protocol plus a CIDR for 7 d (GnRH analogue and CIDR on Day-9, PGF(2alpha) analogue with CIDR removal on Day-2, and GnRH analogue on Day 0), with ET on Day 7. The ovulation synchronization (100%) and embryo transfer (100%) rates in the Ovsynch+CIDR group were greater (P<0.01) than the estrus synchronization (66.7%) and the embryo transfer (61.1%) rates in the PGF group. The postpartum interval at ET in the Ovsynch+CIDR group (62.5 +/- 2.5 d) was shorter (P<0.01) than in the PGF group (74.9 +/- 3.9 d). The pregnancy rate in the Ovsynch+CIDR group (57.9%) did not differ significantly from that in the PGF group (50.0%). Plasma progesterone concentrations were not significantly different in the two groups on Days 0, 1, 2, 5, 7, 14 and 21. In summary, higher synchronization and transfer rates, and shorter postpartum interval to ET, can be achieved with timed ET following the Ovsynch plus CIDR protocol than after estrus with the single PGF(2 alpha) treatment followed by ET in suckled postpartum recipient beef cows. Pregnancy rates were similar. Also, the increase in blood progesterone concentrations during luteal development following ovulation synchronized by the Ovsynch plus CIDR protocol was similar to that after estrus induced by the PGF(2 alpha) treatment.
Kawate
Noritoshi
N
Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, Japan. nkawate@vet.osakafu-u.ac.jp
Sakase
Mitsuhiro
M
Watanabe
Kensuke
K
Fukushima
Moriyuki
M
Noda
Masanobu
M
Takeda
Kazushi
K
Ueno
Satoru
S
Inaba
Toshio
T
Kida
Kayoko
K
Tamada
Hiromichi
H
Sawada
Tsutomu
T
D000282:Administration, Intravaginal; D000818:Animals; D000833:Animals, Suckling; D002417:Cattle; D015237:Dinoprost / Q000008:administration & dosage* / Q000097:blood; D004624:Embryo Transfer / Q000662:veterinary*; D004958:Estradiol / Q000097:blood; D004973:Estrus Synchronization / Q000379:methods*; D005260:Female; D005306:Fertilization / Q000187:drug effects; D007987:Gonadotropin-Releasing Hormone / Q000008:administration & dosage*; D008183:Luteal Phase / Q000187:drug effects; D010060:Ovulation / Q000187:drug effects; D049590:Postpartum Period; D011247:Pregnancy; D011374:Progesterone / Q000097:blood
D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone; D004958:Estradiol; D015237:Dinoprost