title: Hypoglycemic effects of exopolysaccharides produced by mycelial cultures of two different mushrooms Tremella fuciformis and Phellinus baumii in ob/ob mice.
abstract: The anti-diabetic activities of the exopolysaccharides (EPS) produced by submerged mycelial culture of two different mushrooms, Tremella fuciformis and Phellinus baumii, in ob/ob mice were investigated. All the animals were randomly divided into three groups with seven animals in each group: The control group received 0.9% NaCl solution; the diabetic groups were treated with EPS from T. fuciformis (Tf EPS) and P. baumii (Pb EPS) at the level of 200 mg/kg body weight using an oral zoned daily for 52 days. The plasma glucose levels in the EPS-fed mice were substantially reduced by about 52% (Tf EPS) and 32% (Pb EPS), respectively, as compared to control mice. The results of oral glucose tolerance test (OGTT) revealed that both EPS-fed groups significantly increased the glucose disposal after 52 days of EPS treatments. Furthermore, higher food efficiency ratios and reduced blood triglyceride levels were observed in the EPS-treated groups. Because peroxisome proliferator-activated receptor gamma (PPAR-gamma) is indeed a key regulator of insulin action, we investigated the expression pattern of adipose tissue PPAR-gamma messenger RNA (mRNA) and plasma levels of PPAR-gamma. It was revealed that PPAR-gamma was significantly activated in response to EPS treatments. The results suggested that both EPS exhibited considerable hypoglycemic effect and improved insulin sensitivity possibly through regulating PPAR-gamma-mediated lipid metabolism. Our results indicated that two mushroom-derived EPS might be developed as potential oral hypoglycemic agents or functional foods for the management of non-insulin-dependent diabetes mellitus.
authors: Department of Biotechnology, Daegu University, Kyungsan, Kyungbuk 712-714, Republic of Korea.
authors: Eun Jae
authors: EJ
authors: Cho
authors: Hye Jin
authors: HJ
authors: Hwang
authors: Sang Woo
authors: SW
authors: Kim
authors: Jung Young
authors: JY
authors: Oh
authors: Yu Mi
authors: YM
authors: Baek
authors: Jang Won
authors: JW
authors: Choi
authors: Song Hwan
authors: SH
authors: Bae
authors: Jong Won
authors: JW
authors: Yun
mesh_terms: D000818:Animals; D001487:Basidiomycota / Q000502:physiology*; D003920:Diabetes Mellitus / Q000188:drug therapy* / Q000235:genetics; D007004:Hypoglycemic Agents / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D025282:Mycelium / Q000502:physiology; D011134:Polysaccharides / Q000627:therapeutic use*
chemical_list: D007004:Hypoglycemic Agents; D011134:Polysaccharides