title: Genome-wide analyses of the structural gene families involved in the legume-specific 5-deoxyisoflavonoid biosynthesis of Lotus japonicus.
abstract: A model legume Lotus japonicus (Regel) K. Larsen is one of the subjects of genome sequencing and functional genomics programs. In the course of targeted approaches to the legume genomics, we analyzed the genes encoding enzymes involved in the biosynthesis of the legume-specific 5-deoxyisoflavonoid of L. japonicus, which produces isoflavan phytoalexins on elicitor treatment. The paralogous biosynthetic genes were assigned as comprehensively as possible by biochemical experiments, similarity searches, comparison of the gene structures, and phylogenetic analyses. Among the 10 biosynthetic genes investigated, six comprise multigene families, and in many cases they form gene clusters in the chromosomes. Semi-quantitative reverse transcriptase-PCR analyses showed coordinate up-regulation of most of the genes during phytoalexin induction and complex accumulation patterns of the transcripts in different organs. Some paralogous genes exhibited similar expression specificities, suggesting their genetic redundancy. The molecular evolution of the biosynthetic genes is discussed. The results presented here provide reliable annotations of the genes and genetic markers for comparative and functional genomics of leguminous plants.
authors: Department of Applied Biological Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan. nshima18@brs.nihon-u.ac.jp
authors: Norimoto
authors: N
authors: Shimada
authors: Shusei
authors: S
authors: Sato
authors: Tomoyoshi
authors: T
authors: Akashi
authors: Yasukazu
authors: Y
authors: Nakamura
authors: Satoshi
authors: S
authors: Tabata
authors: Shin-Ichi
authors: S
authors: Ayabe
authors: Toshio
authors: T
authors: Aoki
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D004798:Enzymes / Q000235:genetics / Q000378:metabolism; D005419:Flavonoids / Q000096:biosynthesis; D017343:Genes, Plant; D018745:Genome, Plant; D007529:Isoflavones / Q000096:biosynthesis* / Q000737:chemistry; D000070116:Lotus / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D000094162:Phytoalexins
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D004798:Enzymes; D005419:Flavonoids; D007529:Isoflavones; D010940:Plant Proteins; C515147:vestitol; D000094162:Phytoalexins