title: Long-term exposure to glucose and lipids inhibits glucose-induced insulin secretion downstream of granule fusion with plasma membrane.
abstract: Mouse beta-cells cultured at 15 mmol/l glucose for 72 h had reduced ATP-sensitive K+ (K(ATP)) channel activity (-30%), increased voltage-gated Ca2+ currents, higher intracellular free Ca2+ concentration ([Ca2+]i; +160%), more exocytosis (monitored by capacitance measurements, +100%), and greater insulin content (+230%) than those cultured at 4.5 mmol/l glucose. However, they released 20% less insulin when challenged with 20 mmol/l glucose. Glucose-induced (20 mmol/l) insulin secretion was reduced by 60-90% in islets cocultured at 4.5 or 15 mmol/l glucose and either oleate or palmitate (0.5 mmol/l). Free fatty acid (FFA)-induced inhibition of secretion was not associated with any major changes in [Ca2+]i or islet ATP content. Palmitate stimulated exocytosis by twofold or more but reduced K+-induced secretion by up to 60%. Basal (1 mmol/l glucose) K(ATP) channel activity was 40% lower in islets cultured at 4.5 mmol/l glucose plus palmitate and 60% lower in islets cultured at 15 mmol/l glucose plus either of the FFAs. Insulin content decreased by 75% in islets exposed to FFAs in the presence of high (15 mmol/l), but not low (4.5 mmol/l), glucose concentrations, but the number of secretory granules was unchanged. FFA-induced inhibition of insulin secretion was not associated with increased transcript levels of the apoptosis markers Bax (BclII-associated X protein) and caspase-3. We conclude that glucose and FFAs reduce insulin secretion by interference with the exit of insulin via the fusion pore.
authors: Department of Clinical Sciences, Clinical Research Centre, Lund University, Malmö, Sweden.
authors: Charlotta S
authors: CS
authors: Olofsson
authors: Stephan
authors: S
authors: Collins
authors: Martin
authors: M
authors: Bengtsson
authors: Lena
authors: L
authors: Eliasson
authors: Albert
authors: A
authors: Salehi
authors: Kenju
authors: K
authors: Shimomura
authors: Andrei
authors: A
authors: Tarasov
authors: Cecilia
authors: C
authors: Holm
authors: Frances
authors: F
authors: Ashcroft
authors: Patrik
authors: P
authors: Rorsman
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects*; D005947:Glucose / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism*; D007515:Islets of Langerhans / Q000187:drug effects*; D008055:Lipids / Q000494:pharmacology*; D051379:Mice; D019301:Oleic Acid / Q000494:pharmacology; D010168:Palmitates / Q000494:pharmacology; D015221:Potassium Channels / Q000187:drug effects; D022142:Secretory Vesicles / Q000187:drug effects*
chemical_list: D007328:Insulin; D008055:Lipids; D010168:Palmitates; D015221:Potassium Channels; D019301:Oleic Acid; D000255:Adenosine Triphosphate; D005947:Glucose