An antifungal protein from Escherichia coli.
A cytosolic protein was purified from Escherichia coli BL21 that demonstrated potent antifungal activity against pathogenic strains of Aspergillus fumigatus, Aspergillus flavus, Aspergillus niger and Candida albicans. The MIC of purified protein from E. coli BL21 (PPEBL21) against Aspergillus species and C. albicans was 1.95-3.98 and 15.62 microg ml(-1), respectively. In vitro toxicity tests demonstrated no cytotoxicity of PPEBL21 to human erythrocytes up to the tested concentrations of 1250 microg ml(-1). Amphotericin B was lethal to 100 % of human erythrocytes at a concentration of 37.5 microg ml(-1). The N-terminal amino acid sequence of PPEBL21 was found to be DLAEVASR, which showed 75 % sequence similarity with alcohol dehydrogenase of yeast. Mass fingerprinting by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry also substantiated these observations. The results suggested that E. coli BL21 might be an important bioresource of lead molecules for developing new peptide-based therapies for treating fungal infections.
Yadav
V
V
Institute of Genomics and Integrative Biology, Mall Road, University Campus, Delhi, India.
Mandhan
R
R
Department of Biotechnology, Kurukshetra University, Kurukshetra, India.
Pasha
Q
Q
Institute of Genomics and Integrative Biology, Mall Road, University Campus, Delhi, India.
Pasha
S
S
Institute of Genomics and Integrative Biology, Mall Road, University Campus, Delhi, India.
Katyal
A
A
Dr. B.R. Ambedkar Centre for Biomedical Research, Delhi, India.
Chhillar
A K
AK
Shriram Institute for Industrial Research, Delhi, India.
Gupta
J
J
Institute of Genomics and Integrative Biology, Mall Road, University Campus, Delhi, India.
Dabur
R
R
Regional Research Institute (Ay), Kothrud, Pune, India.
Sharma
G L
GL
Institute of Genomics and Integrative Biology, Mall Road, University Campus, Delhi, India.
D000426:Alcohol Dehydrogenase / Q000235:genetics; D000595:Amino Acid Sequence; D000666:Amphotericin B / Q000633:toxicity; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D001230:Aspergillus / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects; D004926:Escherichia coli / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D006801:Humans; D008826:Microbial Sensitivity Tests; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000935:Antifungal Agents; D029968:Escherichia coli Proteins; D000666:Amphotericin B; D000426:Alcohol Dehydrogenase