Fractionation of zein by size exclusion chromatography.
Zein is a group of alcohol-soluble corn proteins, which consists of several individual proteins. A single-step gel filtration chromatography method was developed to fractionate individual zeins from ethanol extracts of whole corn. A Superdex prep 75 column was used with different mobile phases to fractionate the zeins, which were analyzed by SDS-PAGE and UV spectrophotometry. With 70% aqueous ethanol as the mobile phase, fractions containing a mixture of alpha-zein/beta-zein and alpha-zein/delta-zein were obtained. With ammonium bicarbonate added to the 70% ethanol mobile phase, it was possible to obtain beta-zein and delta-zein fractions devoid of other proteins. However, all fractions containing alpha-zein also contained minor amounts of delta-zein and/or beta-zein. Almost all fractions also contained non-protein impurities.
Zhu
Fangyi
F
Agricultural Bioprocess Laboratory, University of Illinois at Urbana-Champaign, 1302 West Pennsylvania Avenue, Urbana, Illinois 61801, USA. fyzhu@uiuc.edu
Kale
Aniket V
AV
Cheryan
Munir
M
D005591:Chemical Fractionation; D002850:Chromatography, Gel / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D012639:Seeds / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D003313:Zea mays / Q000737:chemistry; D015028:Zein / Q000737:chemistry / Q000302:isolation & purification*
D015028:Zein