title: Nuclear envelope precursor vesicle targeting to chromatin is stimulated by protein phosphatase 1 in Xenopus egg extracts.
abstract: The mechanism underlying targeting of the nuclear membrane to chromatin at the end of mitosis was studied using an in vitro cell-free system comprising Xenopus egg membrane and cytosol fractions, and sperm chromatin. The mitotic phase membrane, which was separated from a mitotic phase extract of Xenopus eggs and could not bind to chromatin, became able to bind to chromatin on pretreatment with a synthetic phase cytosol fraction of Xenopus eggs. When the cytosol fraction was depleted of protein phosphatase 1 (PP1) with anti-Xenopus PP1gamma1 antibodies, this ability was lost. The addition of recombinant xPP1gamma1 to the PP1-depleted cytosol fraction restored the ability. These and other results suggested that dephosphorylation of mitotic phosphorylation sites on membranes by PP1 in the synthetic phase cytosol fraction promoted targeting of the membranes to chromatin. On the other hand, a fragment containing the chromatin-binding domain of lamin B receptor (LBR) but not emerin inhibited targeting of membrane vesicles. It was also shown that PP1 dephosphorylates a phosphate group(s) responsible for regulation of the binding of LBR to chromatin. A possible mechanism involving PP1 and LBR for the regulation of nuclear membrane targeting to chromatin was discussed.
authors: Courses of Fundamental Sciences, Graduate School of Science and Technology, Niigata University, Igarashi-2, Niigata 950-2181, Japan.
authors: Hiromi
authors: H
authors: Ito
authors: Yuhei
authors: Y
authors: Koyama
authors: Makoto
authors: M
authors: Takano
authors: Kohei
authors: K
authors: Ishii
authors: Mitsugu
authors: M
authors: Maeno
authors: Kazuhiro
authors: K
authors: Furukawa
authors: Tsuneyoshi
authors: T
authors: Horigome
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D001665:Binding Sites / Q000502:physiology; D002457:Cell Extracts / Q000737:chemistry; D002843:Chromatin / Q000201:enzymology* / Q000648:ultrastructure; D005260:Female; D008561:Membrane Fusion / Q000502:physiology; D008938:Mitosis / Q000502:physiology*; D009685:Nuclear Envelope / Q000201:enzymology* / Q000648:ultrastructure; D009865:Oocytes / Q000201:enzymology* / Q000648:ultrastructure; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D011485:Protein Binding / Q000502:physiology; D054645:Protein Phosphatase 1; D017434:Protein Structure, Tertiary / Q000502:physiology; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D022161:Transport Vesicles / Q000201:enzymology* / Q000648:ultrastructure; D014981:Xenopus; D000097682:Lamin B Receptor
chemical_list: D000906:Antibodies; D002457:Cell Extracts; D002843:Chromatin; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1; D018160:Receptors, Cytoplasmic and Nuclear; D000097682:Lamin B Receptor