Upregulation of dentin matrix protein 1 promoter activities by core binding factor alpha1 in human dental pulp stem cells.
An osteoblast-specific transcription factor, core binding factor alpha1 (Cbfalpha1), is critical for osteoblast and odontoblast differentiation. In this study, the role of Cbfalpha1 in human dentin matrix protein1 (DMP1) gene expression in human dental pulp stem cells (HDPSCs) was investigated. The desired promoter fragments were obtained and cloned into the pGL3-basic vector. It was found that Cbfalpha1 isoforms were predominantly expressed in the cytoplasm of the HDPSCs and reached to the maximum after transfection for 48h. Furthermore, forced overexpression of Cbfalpha1 induced the increase of the luciferase activities of pGL3-P1-6, especially those of pGL3-P(-505to+86) (p<0.05) were the most significant. Then the site-directed mutagenesis of Cbfalpha1 binding sites in the promoter region of nt -505 to +86 resulted in a marked decline of luciferase activities. Thus, our results suggest that Cbfalpha1 upregulates DMP1 gene expression differentially that may contribute to the spatial-temporal expression pattern of DMP1 during odontoblast differentiation.
Pang
Jianliang
J
Department of Stomatology, The General Hospital of the Air Force P.L.A., Beijing 100036, PR China.
Zhang
Yaqing
Y
Ke
Jie
J
Yu
Qing
Q
He
Wenxi
W
Wu
Buling
B
D002454:Cell Differentiation; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism*; D003782:Dental Pulp / Q000166:cytology* / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D009804:Odontoblasts / Q000166:cytology / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*; D015854:Up-Regulation / Q000502:physiology
D050659:Core Binding Factor Alpha 1 Subunit; C513309:DMP1 protein, human; D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins