[Regulatory effect of IkappaBalpha on NF-kappaB activity during viral gene transactivation in patients with steroid responsive simple nephrotic syndrome].
OBJECTIVE
To investigate the expression of inhibitory kappa B alpha (IkappaBalpha) and it's regulatory effect on nuclear factor kappa B (NF-kappaB) during viral gene transactivation triggered by respiratory tract viruses in steroid responsive simple nephrotic syndrome (SRSNS).


METHODS
Children with SRSNS (including at active and remissive stage) were examined for this research project, and healthy children were chosen as controls. TaqMan reverse transcription-PCR (RT-PCR), Western blot, electro-mobility shift assays were performed to study the protein expression of IkappaBalpha gene and the activity of NF-kappaB in peripheral blood mononuclear cells (PBMCs). Simultaneously, RT-PCR was used to detect the gene expression of respiratory tract viruses (including respiratory syncytial virus and influenza virus), and viral antibody was measured by enzyme-linked immunosorbent assay (ELISA).


RESULTS
Comparing with that from SRSNS at remissive stage and healthy children, the activity of NF-kappaB from SRSNS at active stage was much higher statistically (P < 0.05). The positive correlation between NF-kappaB activation and the gene expression of respiratory tract viruses was observed (OR = 27, Kappa coefficient = 0.59, P <0. 05). In addition, levels of IkappaBalpha protein from children with SRSNS at active stage were much lower than those from healthy children and those from children with SRSNS at remissive stage (P < 0.05). There was a linear negative correlation between the activity of NF-kappaB and the protein level of IkappaBalpha (r = -0.884, P < 0.05). On the other hand, levels of IkappaBalpha mRNA from SRSNS at active stage were much higher than those from healthy children, but much lower than those from children with SRSNS at remissive stage (P < 0.05).


CONCLUSIONS
Abnormal expression of IkappaBalpha, which is attributed to regulate activity of NF-kappaB, occurs during viral gene transactivation triggered by respiratory tract viruses in SRSNS.
Tao
Yu-Hong
YH
Department of Pediatrics, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Wang
Zheng
Z
Li
Chan-Sheng
CS
Zhu
Xiao-Shi
XS
D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D005814:Genes, Viral / Q000235:genetics*; D006801:Humans; D020672:I-kappa B Proteins / Q000235:genetics / Q000378:metabolism*; D007223:Infant; D008297:Male; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000378:metabolism*; D009404:Nephrotic Syndrome / Q000188:drug therapy / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012137:Respiratory System / Q000821:virology; D013256:Steroids / Q000494:pharmacology* / Q000627:therapeutic use; D015533:Transcriptional Activation
D020672:I-kappa B Proteins; D016328:NF-kappa B; C000606204:NFKBIA protein, human; D012333:RNA, Messenger; D013256:Steroids; D000072000:NF-KappaB Inhibitor alpha