MALDI-TOF MS of phosphatidylethanolamines: different adducts cause different post source decay (PSD) fragment ion spectra.
Matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS) is increasingly applied to lipids. However, positional acyl chain analysis of lipids by MALDI was so far scarcely described. In this paper, the fragmentation behavior of phosphatidylethanolamine (PE) is investigated by using post-source decay (PSD) MS. In dependence on the investigated adduct, significant differences could be obtained. It will be shown that in particular the negative ion spectra enable the determination of the individual acyl chains as well as their positions (sn-1 or sn-2). Therefore, MALDI-TOF PSD spectra are a real alternative to more sophisticated MS/MS methods.
Fuchs
Beate
B
University of Leipzig, Faculty of Medicine, Institute of Medical Physics and Biophysics, Härtelstr. 16-18, D-04107 Leipzig, Germany.
Schober
Celestina
C
Richter
Grit
G
Süss
Rosmarie
R
Schiller
Jürgen
J
D004355:Drug Stability; D008968:Molecular Conformation; D008970:Molecular Weight; D010714:Phosphatidylethanolamines / Q000737:chemistry* / Q000302:isolation & purification*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D010714:Phosphatidylethanolamines