Antibody response of brown trout Salmo trutta injected with pathogenic Saprolegnia parasitica antigenic extracts.
Brown trout Salmo trutta injected with antigenic extracts from a pathogenic isolate of Saprolegnia parasitica developed specific antibodies that were detected by enzyme-linked immunosorbent assay (ELISA), immunofluorescence (IF) and Western blotting (WB), but not by immunodiffusion (ID). Three groups of five 2 yr old brown trout were injected intraperitoneally with 3 different antigenic extracts: small hyphal fragments (HF) and soluble extracts from sonicated mycelia grown in medium with or without beta-sytosterol (SEB and SE, respectively). In the 2 groups injected with SE and SEB, antibodies were found in 66.7 % of the serum samples by ELISA, 54.5% by IF and 48.5% by WB. In the group injected with HF, only 1 trout survived the experiment, and in this fish only 1 sample was positive by ELISA. The results obtained by ELISA and IF were similar and show that there is cross-reaction between the antigens used. By WB, the proteins most frequently recognised were 2 proteins of 25 and 29 kDa. No significant differences were found in the groups injected with SE or SEB.
Fregeneda-Grandes
J M
JM
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de León, 24071 León, Spain.
Rodríguez-Cadenas
F
F
Carbajal-González
M T
MT
Aller-Gancedo
J M
JM
D000818:Animals; D000906:Antibodies / Q000097:blood; D000941:Antigens / Q000008:administration & dosage / Q000276:immunology; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology; D007239:Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D044762:Saprolegnia / Q000276:immunology*; D014337:Trout / Q000276:immunology* / Q000382:microbiology
D000906:Antibodies; D000941:Antigens