Mesorhizobium spp. are the main microsymbionts of Caragana spp. grown in Liaoning Province of China.
Caragana species are woody legumes widely distributed in the arid regions of China. These plants form root nodules but their nodule bacteria have not been clearly classified. A total of 112 symbiotic bacterial isolates were obtained from four Caragana species grown in Liaoning Province and were characterized with ARDRA, sodium dodecyl sulfate-polyacrylamide gel electrophoresis of proteins, BOX-PCR and sequencing of the 16S rRNA gene. Most of them were classified as Mesorhizobium belonging to 11 putative species. Three isolates were identified as Rhizobium etli and Burkholderia spp. This study offers new information about the Caragana-rhizobia association and resource for selection of inoculants used in sustainable agriculture and for further studies on the Caragana rhizobia.
Yan
Xue Rui
XR
Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture/Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing, China.
Chen
Wen Feng
WF
Fu
Jun Fan
JF
Lu
Yang Li
YL
Xue
Cai Yun
CY
Sui
Xin Hua
XH
Li
Ying
Y
Wang
En Tao
ET
Chen
Wen Xin
WX
D020561:Alphaproteobacteria / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D001426:Bacterial Proteins / Q000032:analysis; D032602:Caragana / Q000254:growth & development / Q000382:microbiology*; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013559:Symbiosis
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S