title: Brain-derived neurotrophic factor modulates cell excitability in the mouse medial nucleus of the trapezoid body.
abstract: Neurotrophins are a large class of trophic factors located throughout the central nervous system. While the role of neurotrophins in neuronal survival and axon guidance is well known, their secondary role in modulating synaptic transmission and cell firing properties is largely unexplored. In this study we examined the expression of neurotrophins in the mouse medial nucleus of the trapezoid body (MNTB) and investigated the effect of exogenous brain-derived neurotrophic factor (BDNF) application on the firing properties of MNTB principal cells. The expression levels of nerve growth factor, BDNF, neurotrophin-3, neurotrophin-4/5 and major receptor tyrosine kinase B was found to be moderate to high at postnatal day 12, indicating that the neurotrophins may have a role following synaptogenesis. A 2-h exposure to exogenous BDNF (100 ng/mL) had a significant effect on principal cell firing properties and voltage-gated potassium currents. Importantly, preincubation in BDNF increased the incidence of multifiring and rebounding cells, and significantly increased the number of action potentials fired in response to a single depolarizing step. BDNF exposure also significantly decreased underlying voltage-gated potassium currents, including both the low- and high-voltage-activated components. Our data show that the neurotrophins, specifically BDNF, may have a novel role in modulating cell excitability in the auditory brainstem.
authors: Division of Neuroscience, John Curtin School of Medical Research, Australian National University, Canberra, ACT, Australia. monique.youssoufian@anu.edu.au
authors: M
authors: M
authors: Youssoufian
authors: B
authors: B
authors: Walmsley
mesh_terms: D000200:Action Potentials / Q000187:drug effects*; D000818:Animals; D000831:Animals, Newborn; D001933:Brain Stem / Q000166:cytology*; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism* / Q000494:pharmacology*; D004307:Dose-Response Relationship, Radiation; D004558:Electric Stimulation; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D008808:Mice, Inbred CBA; D009414:Nerve Growth Factors / Q000378:metabolism; D009474:Neurons / Q000187:drug effects*; D018408:Patch-Clamp Techniques; D011189:Potassium Chloride / Q000494:pharmacology; D020813:Receptor, trkB / Q000378:metabolism
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D011189:Potassium Chloride; D020813:Receptor, trkB