Nidogen is a prosurvival and promigratory factor for adult Schwann cells.
Schwann cells provide a favorable microenvironment for successful regeneration of the injured peripheral nerve. Even though the roles of extracellular matrix proteins in the Schwann cell physiology have long been studied, the precise function of nidogen, a ubiquitous component of the basal lamina, in Schwann cells is unknown. In this study, we show that the protein and mRNA messages for nidogens are up-regulated in the sciatic nerve after sciatic nerve transection. We demonstrate that recombinant nidogen-1 increased the process formation of Schwann cells cultured from adult rat sciatic nerves and that nidogen-1 prevented Schwann cells from serum-deprivation-induced death. In addition, nidogen-1 promoted spontaneous migration of Schwann cells in two-independent migration assays. The Schwann cell responses to the recombinant nidogen-1 were specific because the nidogen-binding ectodomain of tumor endothelial marker 7 inhibited the nidogen responses without affecting Schwann cell response to laminin. Finally, we found that beta1 subunit-containing integrins play a key role in the nidogen-induced process formation, survival, and migration of Schwann cells. Altogether, these results indicate that nidogen has a prosurvival and promigratory activity on Schwann cells in the peripheral nerve.
Lee
Hyun Kyoung
HK
Department of Physiology, Medical Science Research Institute, College of Medicine, Dong-A University, Busan, South Korea.
Seo
In Ae
IA
Park
Hye Kyung
HK
Park
Yoo Mi
YM
Ahn
Kyoung Jin
KJ
Yoo
Young Hyun
YH
Park
Hwan Tae
HT
D000818:Animals; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects* / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D019012:Integrin beta1 / Q000378:metabolism; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D009186:Myelin Sheath / Q000187:drug effects* / Q000502:physiology; D009363:Neoplasm Proteins / Q000494:pharmacology; D009416:Nerve Regeneration / Q000187:drug effects* / Q000502:physiology; D010525:Peripheral Nerves / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D010523:Peripheral Nervous System Diseases / Q000188:drug therapy / Q000378:metabolism / Q000503:physiopathology; D017434:Protein Structure, Tertiary / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D012583:Schwann Cells / Q000187:drug effects* / Q000502:physiology; D020426:Sciatic Neuropathy / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation / Q000502:physiology
D016895:Culture Media, Serum-Free; D019012:Integrin beta1; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; C510620:PLXDC1 protein, human; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins; C029967:nidogen