Nucleotide sequence and polymorphism of the pig acyl coenzyme A synthetase long-chain 1 (ACSL1) gene.
We have sequenced 3,013 bp of the pig acyl coenzyme A long-chain synthetase 1 (ACSL1) gene. Sequence analysis allowed us to identify three conserved elements in the predicted amino acid sequence, two of them related to the ATP/AMP signature motif and the third involved in enzyme catalysis and fatty acid substrate specificity. In addition, we have identified five C --> T and one G --> A transition polymorphisms located in exon 16 (SNPe16), exon 17 (SNPe17) and the 3' UTR (SNPa to d), which have been genotyped in 143 pigs from the Landrace, Large White, Piétrain, Iberian, and Duroc breeds.
Vidal
Oriol
O
Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, Barcelona, Spain. oriol.vidal@uab.es
Sánchez
Armand
A
Amills
Marcel
M
Noguera
José Luis
JL
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003066:Coenzyme A Ligases / Q000235:genetics*; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D013552:Swine / Q000235:genetics* / Q000378:metabolism
D003066:Coenzyme A Ligases