The preventive effect of Daikenchuto on postoperative adhesion-induced intestinal obstruction in rats.
The present study investigated the effect of Daikenchuto (DKT) on postoperative intestinal adhesion in rats. We evaluated the effects of DKT, constituent medical herbs and active compounds on talc-induced intestinal adhesion in rats and DKT-induced contractions using isolated guinea pig ileum. DKT significantly prevented adhesion formation, and this action was inhibited by pretreatment with atropine or ruthenium red. The constituent medical herbs, Zanthoxylum Fruit and Maltose Syrup Powder significantly prevented adhesion formation. Moreover, hydroxy sanshool (HS) prevented adhesion formation, and this action was inhibited by pretreatment with ruthenium red. In contrast, DKT-induced contractions were inhibited by tetrodotoxin, atropine, and capsazepine. These results suggested that DKT had a preventive action on postoperative adhesive intestinal obstruction, and that this action was mediated by sensory and cholinergic nerves. Furthermore, HS was found to be one of the active compound of DKT, and its action was mediated by sensory nerves.
Tokita
Y
Y
Central Research Laboratory, Tsumura & Co., 3586 Yoshiwara, Ami-machi, Inashiki-gun, Ibaraki, Japan. tokita_youhei@mail.tsumura.co.jp
Satoh
K
K
Sakaguchi
M
M
Endoh
Y
Y
Mori
I
I
Yuzurihara
M
M
Sakakibara
I
I
Kase
Y
Y
Takeda
S
S
Sasaki
H
H
D000577:Amides / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D004195:Disease Models, Animal; D005638:Fruit; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects; D007415:Intestinal Obstruction / Q000209:etiology / Q000517:prevention & control*; D008320:Maltose / Q000494:pharmacology; D008518:Medicine, East Asian Traditional; D009119:Muscle Contraction / Q000187:drug effects; D009424:Nervous System Physiological Phenomena; D005894:Panax; D010936:Plant Extracts / Q000494:pharmacology*; D011183:Postoperative Complications / Q000517:prevention & control*; D051381:Rats; D013627:Talc; D000267:Tissue Adhesions / Q000209:etiology / Q000517:prevention & control; D032106:Zanthoxylum / Q000737:chemistry; D030521:Zingiberaceae
D000577:Amides; D010936:Plant Extracts; C121414:dai-kenchu-to; D013627:Talc; C100574:sanshool; D008320:Maltose