Medicago truncatula ENOD40-1 and ENOD40-2 are both involved in nodule initiation and bacteroid development.
The establishment of a nitrogen-fixing root nodule on legumes requires the induction of mitotic activity of cortical cells leading to the formation of the nodule primordium and the infection process by which the bacteria enter this primordium. Several genes are up-regulated during these processes, among them ENOD40. Here it is shown, by using gene-specific knock-down of the two Medicago truncatula ENOD40 genes, that both genes are involved in nodule initiation. Further, during nodule development, both genes are essential for bacteroid development.
Wan
Xi
X
Laboratory of Molecular Biology, Department of Plant Sciences, Wageningen University, Dreijenlaan 3, 6703 HA, Wageningen, The Netherlands.
Hontelez
Jan
J
Lillo
Allessandra
A
Guarnerio
Chiara
C
van de Peut
Diederik
D
Fedorova
Elena
E
Bisseling
Ton
T
Franssen
Henk
H
D001483:Base Sequence; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D046913:Medicago truncatula / Q000235:genetics / Q000254:growth & development* / Q000382:microbiology*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D034622:RNA Interference; D053204:Root Nodules, Plant / Q000235:genetics / Q000254:growth & development*; D016415:Sequence Alignment; D013559:Symbiosis / Q000235:genetics
D010940:Plant Proteins