title: Quantum dot-based mortalin staining as a visual assay for detection of induced senescence in cancer cells.
abstract: Quantum dots (QDs) are fluorescent nanocrystals that are emerging as fine alternatives to the conventional organic dyes. They have several advantages including greater photostability and a wider range of excitation-emission wavelengths. By using mortalin staining as a model, we initially demonstrated that the QDs are more stable and provide better resolution in protein imaging in fixed cells. With the help of an internalizing antibody, we generated internalizing QD (i-QD) and demonstrated its inertness to cell replication, structure, and viability. Based on the superior resolution, stability and inertness, we propose the use of QD staining of mortalin as a cell-based visual assay to screen for senescence-inducing drugs, proteins, and siRNAs.
authors: Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki 305 8562, Japan.
authors: Zeenia
authors: Z
authors: Kaul
authors: Tomoko
authors: T
authors: Yaguchi
authors: Hiroshi X
authors: HX
authors: Chiura
authors: Sunil C
authors: SC
authors: Kaul
authors: Renu
authors: R
authors: Wadhwa
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D001692:Biological Transport; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D002470:Cell Survival; D016922:Cellular Senescence; D004354:Drug Screening Assays, Antitumor; D005456:Fluorescent Dyes / Q000494:pharmacology; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D006801:Humans; D020128:Inhibitory Concentration 50; D008856:Microscopy, Fluorescence / Q000379:methods*; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology*; D045663:Quantum Dots; D034741:RNA, Small Interfering / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D005456:Fluorescent Dyes; D018840:HSP70 Heat-Shock Proteins; D034741:RNA, Small Interfering; C080352:mortalin