Fermentation of plant cell walls by ruminal bacteria, protozoa and fungi and their interaction with fibre particle size.
The objective of the experiment was to evaluate the contribution of various ruminal microbial groups to the fermentation of cell walls of corn stover with different particle sizes based on ruminal gas production in vitro. Physical, chemical, and antibiotical methods were used to differentiate groups of bacteria, protozoa and fungi in rumen fluid, offering following rumen microbial groups: whole rumen fluid (WRF), bacterial (B), protozoal (P), fungal (F), bacterial plus protozoal (B + P), bacterial plus fungal (B + F), protozoal plus fungal (P + F), and negative control (CON). Cell walls from corn stover were ground and ball milled to produce two different particle sizes. The results showed that digestion of the cell walls was undertaken by the interaction among ruminal bacteria, protozoa and fungi, and such co-actions seemed to fail alternation by one of three microbial groups or any combinations. However, B + P group showed a significant contribution to the degradation of milled cell walls, and B + F group revealed a great synergy effect on the ground cell walls degradation. Particle size of cell walls also had a considerable influence on their fermentation extent instead of the fermentative patterns by various rumen microbial groups.
Zhang
Yuanqing
Y
State Key Laboratory of Animal Nutrition, Beef Cattle Research Centre and College of Animal Science and Technology, China Agricultural University, Beijing, PR China.
Gao
Wei
W
Meng
Qingxiang
Q
D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001421:Bacteria, Anaerobic / Q000254:growth & development / Q000378:metabolism*; D002417:Cattle; D002473:Cell Wall / Q000378:metabolism*; D004043:Dietary Fiber / Q000378:metabolism*; D056890:Eukaryota / Q000254:growth & development / Q000378:metabolism*; D005285:Fermentation; D005658:Fungi / Q000254:growth & development / Q000378:metabolism*; D008297:Male; D010316:Particle Size; D012417:Rumen / Q000378:metabolism / Q000382:microbiology / Q000469:parasitology; D013997:Time Factors; D003313:Zea mays