[Effect of human silicotic alveolar macrophages on the expression of the collagen type I in human embryonic lung fibroblasts].
OBJECTIVE
To study the effect of the cultured supernatant of human silicotic alveolar macrophages (AM) on the expression of the collagen type I in human embryonic lung fibroblasts.


METHODS
Human alveolar macrophages were collected from a silicotic patient by bronchoalveolar lavage and exposed to silicon dioxide for 18 h. Then the cultured supernatant were used to culture human embryonic lung fibroblasts for 6 h, 12 h, 18 h, 24 h, 36 h, 48 h, 72 h. Then detected collagen anabolism and secretion with (3)H-proline detected the expression of the procollagen type I in the fibroblast with immunological method detected the quantity of collagen Type I in FB supernatant with Western blot.


RESULTS
The anabolism and secretion of collagen were increased in cultured supernatant of silicotic AM exposed to SiO(2), Along with the time, the expression of collagen type I increased. In cultured supernatant of silicotic AM exposed to SiO(2), ((3)H-proline: 1096.500 +/- 76.400, 707.000 +/- 62.160, OD: 0.314 +/- 0.011, OD: 14.218 +/- 0.342.


CONCLUSIONS
SiO(2) may affect the expression of collagen through AM mediation and participate in the formation of lung fibrosis.
Hao
Xiao-hui
XH
North China Coal Medical College, Tangshan 063000, China.
Wang
Xian-hua
XH
Zhang
Li
L
Yang
Fang
F
D000328:Adult; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D008168:Lung / Q000378:metabolism; D016676:Macrophages, Alveolar / Q000276:immunology*; D008297:Male; D012829:Silicosis / Q000276:immunology*
D024042:Collagen Type I