title: Identification of marker genes distinguishing human periodontal ligament cells from human mesenchymal stem cells and human gingival fibroblasts.
abstract: OBJECTIVE
  Molecular gene markers, which can distinguish human bone marrow mesenchymal stem cells from human fibroblasts, have recently been reported. Messenger RNA levels of tissue factor pathway inhibitor-2, major histocompatibility complex-DR-alpha, major histocompatibility complex-DR-beta, and neuroserpin are higher in human bone marrow mesenchymal stem cells than in human fibroblasts. However, human bone marrow mesenchymal stem cells express less apolipoprotein D mRNA than human fibroblasts. Periodontal ligament cells are a heterogeneous cell population including fibroblasts, mesenchymal stem cells, and progenitor cells of osteoblasts or cementoblasts. The use of molecular markers that distinguish human bone marrow mesenchymal stem cells from human fibroblasts may provide insight into the characteristics of human periodontal ligament cells. In this study, we compared the molecular markers of human periodontal ligament cells with those of human bone marrow mesenchymal stem cells and human gingival fibroblasts.
  
  
  METHODS
  The mRNA expression of the molecular gene markers was analyzed using real-time polymerase chain reaction. Statistical differences were determined with the two-sided Mann-Whitney U-test.
  
  
  RESULTS
  Messenger RNA levels of major histocompatibility complex-DR-alpha and major histocompatibility complex-DR-beta were lower and higher, respectively, in human periodontal ligament cells than in human bone marrow mesenchymal stem cells or human gingival fibroblasts. Human periodontal ligament cells showed the lowest apolipoprotein D mRNA levels among the three types of cells.
  
  
  CONCLUSIONS
  Human periodontal ligament cells may be distinguished from human bone marrow mesenchymal stem cells and human gingival fibroblasts by the genes for apolipoprotein D, major histocompatibility complex-DR-alpha, and major histocompatibility complex-DR-beta.
authors: Department of Periodontal Medicine, Division of Frontier Medical Science, Hiroshima University Graduate School of Biomedical Science, Hiroshima, Japan. tfuji@hiroshima-u.ac.jp
authors: T
authors: T
authors: Fujita
authors: T
authors: T
authors: Iwata
authors: H
authors: H
authors: Shiba
authors: A
authors: A
authors: Igarashi
authors: R
authors: R
authors: Hirata
authors: K
authors: K
authors: Takeda
authors: N
authors: N
authors: Mizuno
authors: K
authors: K
authors: Tsuji
authors: H
authors: H
authors: Kawaguchi
authors: Y
authors: Y
authors: Kato
authors: H
authors: H
authors: Kurihara
mesh_terms: D053399:Apolipoproteins D / Q000235:genetics; D001854:Bone Marrow Cells / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology*; D005819:Genetic Markers / Q000235:genetics; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D008285:Major Histocompatibility Complex / Q000235:genetics; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010513:Periodontal Ligament / Q000166:cytology*; D012333:RNA, Messenger / Q000032:analysis; D018709:Statistics, Nonparametric
chemical_list: D053399:Apolipoproteins D; D005819:Genetic Markers; D012333:RNA, Messenger