Oxygen isotope enrichment of organic matter in Ricinus communis during the diel course and as affected by assimilate transport.
The oxygen isotope composition in leaf water and organic compounds in different plant tissues is useful for assessing the physiological performance of plants in their environment, but more information is needed on Delta(18)O variation during a diel course. Here, we assessed Delta(18)O of the organic matter in leaves, phloem and xylem in stem segments, and fine roots of Ricinus communis during a full diel cycle. Enrichment of newly assimilated organic matter in equilibrium with leaf water was calculated by applying a nonsteady-state evaporative enrichment model. During the light period, Delta(18)O of the water soluble organic matter pool in leaves and phloem could be explained by a 27 per thousand enrichment compared with leaf water enrichment. Leaf water enrichment influenced Delta(18)O of phloem organic matter during the night via daytime starch synthesis and night-time starch remobilization. Phloem transport did not affect Delta(18)O of phloem organic matter. Diel variation in Delta(18)O in organic matter pools can be modeled, and oxygen isotopic information is not biased during transport through the plant. These findings will aid field studies that characterize environmental influences on plant water balance using Delta(18)O in phloem organic matter or tree rings.
Gessler
Arthur
A
Environmental Biology Group; Research School of Biological Sciences, Australian National University, Canberra, Australia.|School of Forest and Ecosystem Science, University of Melbourne, Water Street, Creswick, Victoria, Australia.|Present address: Core Facility Metabolomics, Centre for Systems Biology (ZBSA) and Chair of Tree Physiology, University of Freiburg, Georges-Köhler Allee 53/54, 79085 Freiburg im-Bresigau, Germany.
Peuke
Andreas D
AD
School of Biological, Earth and Environmental Sciences, University of New South Wales, Sydney NSW 2052 Australia.
Keitel
Claudia
C
Environmental Biology Group; Research School of Biological Sciences, Australian National University, Canberra, Australia.
Farquhar
Graham D
GD
Environmental Biology Group; Research School of Biological Sciences, Australian National University, Canberra, Australia.
D001692:Biological Transport; D002367:Ricinus communis / Q000378:metabolism*; D002940:Circadian Rhythm; D010103:Oxygen Isotopes / Q000378:metabolism*; D052585:Phloem / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D018547:Plant Stems / Q000378:metabolism
D010103:Oxygen Isotopes