Phenotypes of IRS-2 deficient mice produced by reproductive technology are stable.
We studied the impact of "IVF - ET" on the glucose tolerance test (GTT), insulin tolerance test (ITT) and adiponectin to investigate differences in the phenotypes of B6J- Irs2(-/-) mice. The B6J-Irs2(-/-) mice (KO-Nat group) were prepared by natural mating. Other mice were produced by IVF-ET used ICR strain recipients and surrogate mothers (KO-IVF group). Measurement of body weight, GTT, ITT and blood sampling were performed at the ages of 6, 14 and 24 weeks after birth. Body weights, impaired glucose tolerance, insulin resistance and plasma adiponectin concentrations did not differ for each gender between the KO-IVF and KO-Nat groups. Therefore, we concluded that phenotypes of Irs2(-/-) mice produced by reproductive technology are stable.
Hashimoto
Haruo
H
Central Institute for Experimental Animals, Kawasaki-Shi, Kanagawa, Japan.
Arai
Toshiro
T
Ohnishi
Yasuyuki
Y
Eto
Tomoo
T
Ito
Mamoru
M
Hioki
Kyoji
K
Suzuki
Ryo
R
Yamauchi
Toshimasa
T
Ohsugi
Mitsuru
M
Saito
Muneo
M
Ueyama
Yoshito
Y
Tobe
Kazuyuki
K
Kadowaki
Takashi
T
Tamaoki
Norikazu
N
Kosaka
Kinori
K
D052242:Adiponectin / Q000097:blood; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight / Q000235:genetics; D003307:Copulation / Q000502:physiology*; D003921:Diabetes Mellitus, Experimental / Q000235:genetics / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro; D020868:Gene Silencing; D005951:Glucose Tolerance Test; D007178:Inbreeding; D055504:Insulin Receptor Substrate Proteins; D007333:Insulin Resistance / Q000235:genetics; D047908:Intracellular Signaling Peptides and Proteins / Q000097:blood / Q000172:deficiency / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010641:Phenotype; D010750:Phosphoproteins / Q000097:blood / Q000172:deficiency / Q000235:genetics*; D011972:Receptor, Insulin / Q000097:blood / Q000172:deficiency / Q000235:genetics*; D013047:Specific Pathogen-Free Organisms
D052242:Adiponectin; D001786:Blood Glucose; D055504:Insulin Receptor Substrate Proteins; D047908:Intracellular Signaling Peptides and Proteins; C526298:Irs2 protein, mouse; D010750:Phosphoproteins; D011972:Receptor, Insulin