title: [Experiment research on the location of DNA lesion in the nucleic acid sequence].
abstract: OBJECTIVE
  To research on establishing a method that can detect the DNA lesion at the level of nucleic acid.
  
  
  METHODS
  The TK6 cell was cultured and treated, the genomic DNA was extracted, and the strand cleavage was induced by the endonuclease Hinf I. Then the genomic DNA was amplified by randomized terminal linker-dependent PCR (RDPCR), and hybridized by Southern hybridization with the single-stranded probes of the exon 2 of k-ras gene. The PCR products were sequenced.
  
  
  RESULTS
  The clear hybridized band from the products of RDPCR was seen at the expectant position cut by Hinf I. The sequencing analysis showed that the position of DNA lesion linked by the linker was just the restriction site of Hinf I.
  
  
  CONCLUSIONS
  It can be used to detect the DNA lesion at the level of nucleic acid sequence with the combination of sequence and RDPCR technologies.
authors: Department of Environmental Health, West China School of Public Health, Sichuan University, Chengdu 610041, China.
authors: Xu-Fang
authors: XF
authors: Gao
authors: Cao-Long
authors: CL
authors: Rao
authors: Wei-Hua
authors: WH
authors: Wen
authors: Zheng-Cang
authors: ZC
authors: Heng
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D004249:DNA Damage; D005091:Exons / Q000235:genetics; D006801:Humans; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D004247:DNA