Lab-on-a-chip technologies for oral-based cancer screening and diagnostics: capabilities, issues, and prospects.
The design of a microfluidic lab-on-a-chip system for point-of-care cancer screening and diagnosis of oral squamous cell carcinoma (OSCC) is presented. The chip is based on determining a approximately 30-gene transcription profile in cancer cells isolated from oral fluid samples. Microfluidic cell sorting using magnetic beads functionalized with an antibody against cancer-specific cell-surface antigens (e.g., epithelial cell adhesion molecule [EpCAM]) is described. A comprehensive cancer diagnostics chip will integrate microfluidic components for cell lysis, nucleic acid extraction, and amplification and detection of a panel of mRNA isolated from a subpopulation of cancer cells contained in a clinical specimen.
Mauk
Michael G
MG
Department of Mechanical Engineering and Applied Science, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6315, USA.
Ziober
Barry L
BL
Chen
Zongyuan
Z
Thompson
Jason A
JA
Bau
Haim H
HH
D000912:Antibodies, Neoplasm / Q000032:analysis; D000951:Antigens, Neoplasm / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000737:chemistry / Q000175:diagnosis* / Q000473:pathology; D005240:Feasibility Studies; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008403:Mass Screening; D046208:Microchip Analytical Procedures / Q000191:economics; D044085:Microfluidics; D009062:Mouth Neoplasms / Q000737:chemistry / Q000175:diagnosis* / Q000473:pathology; D011237:Predictive Value of Tests
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm