[Research on the classification of streptomyces strain ZG0429 and purification of streptavidin].
Actinomycete strain ZG0429 with high production of streptavidin was isolated from soil samples. Based on the results of morphological, physiological, chemotaxonomic characteristics and 16S rRNA analysis, strain ZG0429 was placed within the genus Streptomyces and identified as the species Streptomyces lavendulae. The production reached 201.0mg/L under our fermentation conditions. With the effective system of ammonium-sulfate deposition and gel filtration chromatography, streptavidin was successfully purified from the culture supernatant with an overall recovery of 76.87% and purity of 97.03% . This study illustrates an effective and inexpensive method to produce the aimed protein with favorable yield, high purity and desirable activity, so it provides a valuable reference for the large-scale industrial production and isolation of streptavidin.
Zhang
Lei
L
College of Life Sciences, Nankai University, Tianjin 300071 , China.
Liu
Chun-qin
CQ
Gao
Zhi-hui
ZH
Cao
Yu
Y
Bai
Gang
G
D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019809:Streptavidin / Q000302:isolation & purification*; D013302:Streptomyces / Q000145:classification* / Q000254:growth & development
D012336:RNA, Ribosomal, 16S; D019809:Streptavidin