Evaluation of peanut genotypes for in vitro plant regeneration using thidiazuron.
A major limitation with the available protocols for in vitro regeneration of peanut (Arachis hypogaea L.) is their narrow application to very few select genotypes. Here, we report a protocol that can be applied across a broad spectrum of peanut market types, explant types and geographic regions using thidiazuron (TDZ). The effect of the timing of TDZ application to the culturing of both zygotic embryos and subsequent plantlet explants on MS medium is also reported. An extended use of TDZ and at a higher concentration (30 m/l) resulted in the greatest explant shoot average (approximately 13). However, a limited application of TDZ (10 d) was sufficient to induce shoot formation in peanut. Hypocotyl was the best explant type that induced the greatest shoot average (15) across market types followed by lamina (7.4). Spanish and Valencia were the most efficient market groups that induced shoots across explant types, consistently.
Matand
Kanyand
K
Center for Biotechnology Research and Education, School of Agriculture and Applied Sciences, Langston University, Langston, OK 73050, USA. kmatand@luresext.edu
Prakash
C S
CS
D010367:Arachis / Q000196:embryology* / Q000235:genetics / Q000502:physiology*; D003470:Culture Media; D046508:Culture Techniques; D005838:Genotype; D010671:Phenylurea Compounds / Q000494:pharmacology*; D018520:Plant Shoots / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D012038:Regeneration / Q000187:drug effects*; D013830:Thiadiazoles / Q000494:pharmacology*
D003470:Culture Media; D010671:Phenylurea Compounds; D013830:Thiadiazoles; C016785:thidiazuron