Protein immobilization on aminated poly(glycidyl methacrylate) nanofibers as polymeric carriers.
Recently, protein carriers based on nanomaterials have been highlighted in diverse biological applications such as protein extraction, separation, and delivery due to their facile gravimetric sedimentation in the aqueous phase and abundant surface functionalities, which were used as anchoring sites for proteins. From this viewpoint, poly(glycidyl methacrylate) nanofibers (PGMA NFs) can be an excellent candidate for protein support because PGMA NFs possess the activated epoxide functional groups on the surface. In addition, cured PGMA NFs (PGMA-NH2 NFs) reveal different surface functionalities such as primary amine groups. They can be linked with carboxylated proteins. Ferritin and streptavidin were selected as models of the pristine and biolinker-mediated proteins in this experiment and immobilized onto PGMA NFs and aminated PGMA-NH2 NFs. The successful conjugations of ferritin and streptavidin were confirmed with transmission electron microscopy and fluorescein-isothiocyanate-tagged molecules. Protein immobilization using the pristine and the cured PGMA NFs could be considered as an outstanding protocol for facile protein delivery.
Ko
Sungrok
S
Hyperstructured Organic Materials Research Center, School of Chemical and Biological Engineering, College of Engineering, Seoul National University, 56-1 Shinlimdong, Seoul 151-742, South Korea.
Jang
Jyongsik
J
D005293:Ferritins / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry; D013499:Surface Properties
D011109:Polymethacrylic Acids; D011506:Proteins; C042535:polyglycidyl methacrylate; D005293:Ferritins; D019809:Streptavidin