title: Association between CD4(+)CD25(high) T cells and atopy in children.
abstract: BACKGROUND
  There is evidence that CD4(+)CD25(high) T-regulatory cells are important for establishing tolerance to allergens, but information in children is limited.
  
  
  OBJECTIVE
  To test the hypothesis that greater numbers and function of CD4(+)CD25(high) T cells are associated with a reduced risk of childhood allergies and wheezing.
  
  
  METHODS
  A cohort of 151 six-year-old children from atopic families was analyzed for peripheral blood CD4(+)CD25(high) and CD4(+)CD25(int) T cells by flow cytometry and for clinical and immunologic correlates of atopy. The associations between these variables were assessed by regression analysis.
  
  
  RESULTS
  Factors positively associated with % CD4(+)CD25(high)/CD4 T cells were male sex, number of positive allergen-specific IgE tests, total IgE, season, and 1-month average total pollen count preceding blood draw. The percentage of CD4(+)CD25(high)/total CD4 T cells did not correlate with induced cytokine production, and correlated negatively with suppressive capacity of CD4(+)CD25(+) T cells (r = -0.45; P = .034). The percentage of CD4(+)CD25(int)/CD4 T cells was 54% higher in pollen-sensitized children compared with nonsensitized children in spring (P = .023 for interaction), and correlated positively with IL-5, IL-10, and IL-13 (P < or = .001 for all).
  
  
  CONCLUSIONS
  Our findings suggest that blood CD4(+)CD25(high) cells are a mixture of activated and regulatory T cells, and that these cells could be seasonally regulated by environmental factors such as pollen exposure.
  
  
  CONCLUSIONS
  Seasonal increases in CD4CD25(high) expression in children with allergy may represent systemic immune activation caused by pollen exposures.
authors: Department of Pediatrics, University of Wisconsin, Madison, WI 53792-4108, USA.
authors: Tuomas
authors: T
authors: Jartti
authors: Kristjan A
authors: KA
authors: Burmeister
authors: Christine M
authors: CM
authors: Seroogy
authors: Martha L
authors: ML
authors: Jennens-Clough
authors: Christopher J
authors: CJ
authors: Tisler
authors: Lisa P
authors: LP
authors: Salazar
authors: Douglas F
authors: DF
authors: Dasilva
authors: Michael D
authors: MD
authors: Evans
authors: Rose F
authors: RF
authors: Vrtis
authors: Paul K
authors: PK
authors: Wallace
authors: Begona
authors: B
authors: Ruiz-Perez
authors: Ronald E
authors: RE
authors: Gangnon
authors: Robert F
authors: RF
authors: Lemanske
authors: James E
authors: JE
authors: Gern
mesh_terms: D002648:Child; D005260:Female; D051858:Forkhead Transcription Factors / Q000378:metabolism; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000175:diagnosis / Q000276:immunology*; D053645:Interleukin-2 Receptor alpha Subunit / Q000032:analysis / Q000378:metabolism; D008297:Male; D012621:Seasons; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
chemical_list: C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D053645:Interleukin-2 Receptor alpha Subunit