Identification and analysis of in vitro cultured CD45-positive cells capable of multi-lineage differentiation.
We report on a subset of cells that co-purify with CD45-positive/Lineage minus (CD45(pos)/Lin(minus)) hematopoietic cells that are capable of in vitro differentiation into multi-potential cells including cells with neuroectoderm properties. Although these cells are CD45 positive and have properties similar to CD45-negative mesenchymal progenitor cells (MPC) derived from bone marrow (BM), they are neither hematopoietic cells nor mesenchymal cells. These CD45(pos)/Lin(minus) cells can be expanded in vitro, express the stem cell genes Oct-4 and Nanog and can be induced to differentiate into endothelial cells, osteoblasts, muscle cells and neural cells at frequencies similar to those reported for bone marrow mesenchymal cells. Long-term culture of these cells followed by transplantation into NOD/SCID mice resulted in positive bone marrow stromal cell engraftment but not hematopoietic engraftment, suggesting that despite their CD45-positive status these cells do not have the same properties as hematopoietic stem cells. Clonal cell analysis determined that the culture period caused a broadening in the differentiation potential of the starting population.
Rogers
Ian
I
Department of Obstetrics and Gynaecology, Rm. 876 Samuel Lunenfeld Research Institute, Mount Sinai Hospital and the University of Toronto, 600 University Ave, Toronto, Ontario, Canada M5G 1X5. rogers@mshri.on.ca
Yamanaka
Nobuko
N
Bielecki
Ryszard
R
Wong
Christine J
CJ
Chua
Shawn
S
Yuen
Shelia
S
Casper
Robert F
RF
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology*; D019070:Cell Lineage / Q000502:physiology*; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology; D006085:Graft Survival / Q000502:physiology; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods; D018398:Homeodomain Proteins / Q000378:metabolism; D006801:Humans; D017493:Leukocyte Common Antigens / Q000276:immunology*; D051379:Mice; D016513:Mice, SCID; D032342:Muscle Cells / Q000166:cytology / Q000502:physiology; D000071317:Nanog Homeobox Protein; D009474:Neurons / Q000166:cytology / Q000502:physiology; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology; D013234:Stem Cells / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology
D015415:Biomarkers; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3; D017493:Leukocyte Common Antigens