[Effect of rosiglitazone on integrin beta1 expression and apoptosis of proximal tubular cell exposed to high glucose].
OBJECTIVE
To evaluate the effect of rosiglitazone (ROS) on integrin beta1 expression and apoptosis of proximal tubular cell exposed to high glucose.


METHODS
The proximal tubular cells of rats were cultured in vitro and divided into 6 groups: control group, normal glucose (5 mmol/L) group, high glucose (30 mmol/L) group, only ROS (10 micromol/L) group, glucose (30 mmol/L)+ROS (5 micromol/L) group, and glucose (30 mmol/L) +ROS (10 micromol/L) group. The cells were cultured for 48 hrs and the integrin beta1 expressions were detected by Western blot and RT-PCR; The apoptosis was evaluated by flow cytometry after the cells were cultured for 24, 48, 72 hrs.


RESULTS
The expressions of integrin beta1 protein and mRNA of high glucose (30 mmol/L) group were significantly increased as compared with control group and normal glucose (5 mmol/L) group. The integrin beta1 of glucose (30 mmol/L)+ROS (5 or 10 micromol/L) group was decreased as compared with high glucose group (P < 0.05). Flow cytometry demonstrated the decreased apoptosis to be with time-dependence.


CONCLUSIONS
ROS can strikingly inhibit the expression of integrin beta1 in proximal tubular cells exposed to high glucose, with a marked dose-dependent manner. The effect of ROS on cell apoptosis may be relevant to integrin beta1.
Tang
Xiao-Hong
XH
Department of Nephrology, West China Hospital, Sichuan University, Chengdu 610041, China.
Huang
Song-Min
SM
Tan
Shi-Qiao
SQ
Ma
Ya-Li
YL
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D048909:Diabetes Complications / Q000517:prevention & control; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology*; D019012:Integrin beta1 / Q000235:genetics* / Q000378:metabolism*; D007687:Kidney Tubules, Proximal / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D000077154:Rosiglitazone; D045162:Thiazolidinediones / Q000494:pharmacology*
D019012:Integrin beta1; D012333:RNA, Messenger; D045162:Thiazolidinediones; D000077154:Rosiglitazone; D005947:Glucose