title: D-box is required for the degradation of human Shugoshin and chromosome alignment.
abstract: Chromosome segregation and proper alignment in mitosis relies on cohesion between sister chromatids and the interaction of the kinetochore with spindle microtubules. Vertebrate Sgo 1 localizes to kinetochores and is required to prevent premature sister centromere separation in mitosis. Sgo 1 is degraded by the anaphase-promoting complex, allowing the separation of sister centromeres in anaphase. However, little is known about the molecular basis of Sgo 1 degradation and its temporal control during mitosis. Here, we show that APC/C targets human Sgo 1 for degradation through a destruction box motif (D-box) in its C-terminus. Mutation in the D-box causes transient metaphase arrest, and mutation in the D-box leads to defects in chromosome alignment and segregation through its effect on the localization of Aurora B and CENP-E. These results provide a link between sister centromere cohesion and bipolar attachment of kinetochores.
authors: Laboratory of Cellular Dynamics, University of Science & Technology of China, Hefei National Laboratory, Hefei 230027, China.
authors: Guosheng
authors: G
authors: Fu
authors: Shasha
authors: S
authors: Hua
authors: Tarsha
authors: T
authors: Ward
authors: Xia
authors: X
authors: Ding
authors: Yong
authors: Y
authors: Yang
authors: Zhen
authors: Z
authors: Guo
authors: Xuebiao
authors: X
authors: Yao
mesh_terms: D000595:Amino Acid Sequence; D000705:Anaphase / Q000502:physiology; D064107:Aurora Kinase B; D064127:Aurora Kinases; D001665:Binding Sites; D015153:Blotting, Western; D002453:Cell Cycle / Q000502:physiology; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002460:Cell Line; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D020090:Chromosome Segregation / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D018613:Microscopy, Confocal; D008938:Mitosis / Q000502:physiology; D009154:Mutation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D014162:Transfection
chemical_list: D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D011993:Recombinant Fusion Proteins; C496644:SGO1 protein, human; C068688:centromere protein E; D049452:Green Fluorescent Proteins; C576921:AURKB protein, human; D064107:Aurora Kinase B; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases