Myosin inhibitors block accumulation movement of chloroplasts in Arabidopsis thaliana leaf cells.
Chloroplasts alter their distribution within plant cells depending on the external light conditions. Myosin inhibitors 2,3-butanedione monoxime (BDM), N-ethylmaleimide (NEM), and 1-(5-iodonaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine hydrochloride (ML-7) were used to study the possible role of myosins in chloroplast photorelocation in Arabidopsis thaliana mesophyll cells. None of these agents had an effect on the chloroplast high-fluence-rate avoidance movement but all of the three myosin inhibitors blocked the accumulation movement of chloroplasts after a high-fluence-rate irradiation of the leaves. The results suggest that myosins have a role in A. thaliana chloroplast photorelocation.
Paves
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Department of Gene Technology, Tallinn University of Technology, Tallinn, Estonia. heiti.paves@ttu.ee
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D017360:Arabidopsis / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D001381:Azepines / Q000494:pharmacology; D016764:Cell Polarity / Q000187:drug effects / Q000502:physiology; D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D003595:Cytoplasmic Streaming / Q000187:drug effects / Q000502:physiology; D003931:Diacetyl / Q000031:analogs & derivatives / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005033:Ethylmaleimide / Q000494:pharmacology; D008027:Light; D009218:Myosins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009281:Naphthalenes / Q000494:pharmacology; D010775:Photic Stimulation; D010788:Photosynthesis / Q000187:drug effects / Q000502:physiology*; D019441:Plant Epidermis / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D018515:Plant Leaves / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
D001381:Azepines; D004791:Enzyme Inhibitors; D009281:Naphthalenes; C070571:ML 7; C004717:diacetylmonoxime; D009218:Myosins; D003931:Diacetyl; D005033:Ethylmaleimide