[Cysteinyl leukotriene receptor 1 antagonist pranlukast modulates differentiation of SK-N-SH cells].
To determine whether cysteinyl leukotriene receptor agonist LTD(4) and cysteinyl leukotriene receptor 1 (CysLT(1)) antagonist pranlukast affect the differentiation of human neuroblastoma SK-N-SH cells.



SK-N-SH cell morphological changes induced by LTD(4), pranlukast and LTD(4) + pranlukast were observed with retinoid acid (RA) as the positive control. The expressions of CysLT(1) and CysLT(2) receptors were detected by immunoblotting analysis, and the expression of microtubule-associated protein-2 (MAP-2), a neuron marker, was detected by fluorescent immunostaining.



The immunoblotting results showed that SK-N-SH cells expressed CysLT(1) receptor moderately, and CysLT(2) receptor highly. The morphological results showed that RA, pranlukast and LTD(4) + pranlukast induced the compaction of the cell bodies and the outgrowth of neurites, while LTD(4) had no significant effect. The immunostaining results showed that MAP-2 was distributed in the cell bodies in control or pranlukast-treated cells; it was distributed in cell bodies and neuritis in RA-treated cells. Pranlukast increased the numbers of MAP-2-positive cells.



The CysLT(1)receptor antagonist pranlukast modulates the differentiation of SK-N-SH cells.
Peng
Fang
F
Department of Pharmacology, College of Medicine, Zhejiang University, Hangzhou 310058, China.
Fang
San-Hua
SH
Zheng
Xiao-Liang
XL
Zhang
Wei-Ping
WP
Lu
Yun-Bi
YB
Wei
Er-Qing
EQ
D002454:Cell Differentiation / Q000187:drug effects*; D045744:Cell Line, Tumor; D002867:Chromones / Q000494:pharmacology*; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D020024:Leukotriene Antagonists / Q000494:pharmacology*; D017998:Leukotriene D4 / Q000494:pharmacology; D008565:Membrane Proteins / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009447:Neuroblastoma / Q000378:metabolism / Q000473:pathology; D018077:Receptors, Leukotriene / Q000378:metabolism*
D002867:Chromones; D020024:Leukotriene Antagonists; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; D018077:Receptors, Leukotriene; D017998:Leukotriene D4; C083169:leukotriene D4 receptor; C047681:pranlukast