Candida rugosa lipase-catalyzed polyurethane degradation in aqueous medium.
Candida rugosa lipase (EC 3.1.1.3) was used to degrade commercially-available solid poly(ester)urethane (Impranil) in an aqueous medium under different temperature, pH, enzyme and substrate concentrations. A mathematical model was developed and applied to represent the degradation kinetics of the solid polyurethane. Reaction optima were found to be pH 7 and 35 degrees C. Diethylene glycol, a degradation byproduct, generation rate was measured to be 0.12 mg/l min and the activation energy was calculated as 9.121 kcal/gmol K. This information will be useful in developing bioreactors for practical applications to manage polyurethane wastes using lipase.
Gautam
Rajeeb
R
Department of Chemical and Biochemical Engineering, The University of Western Ontario, London, ON, Canada.
Bassi
Amarjeet S
AS
Yanful
Ernest K
EK
D002175:Candida / Q000201:enzymology*; D002384:Catalysis; D005026:Ethylene Glycols / Q000378:metabolism; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D011140:Polyurethanes / Q000737:chemistry / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
D005026:Ethylene Glycols; D011140:Polyurethanes; C013484:diethylene glycol; D008049:Lipase