Electrochemical investigation of the effect of some organic phosphates on haemoglobin.
The effects of DPG,IHP,GTP,GDP and GMP on the structure and stability of haemoglobin were electrochemically investigated with an iodide-modified silver electrode in 0.01 M KNO 3 at pH 7.0.Anodic and cathodic peaks of haemoglobin were observed at 250 mV and 12 mV with a formal potential value of 133 mV vs.Ag/AgCl.The effects of different concentrations of DPG,IHP,GTP,GDP and GMP on the anaerobic redox reaction were determined. The results showed that DPG and IHP can lead to a positive shift in the reduction peak of haemoglobin,indicating that the oxidation peak shift of haemoglobin was small as a result of stabilization of the reduced state and destabilization of the R-like state of haemoglobin.GTP elicited a more positive shift in the cathodic and anodic peaks of haemoglobin at a higher concentration,signifying that it has a low-affinity binding site on haemoglobin.The positive shift of the cathodic and anodic peaks revealed a slight variation in the structure and indicated the unfolding of haemoglobin in the presence of high concentrations of GTP.Our study also showed that GDP and GMP did not cause significant shift the cathodic and anodic peaks of haemoglobin even at high concentrations,refuting the existence of specific GDP-and GMP-binding sites on the protein.Moreover,the iodide-modified silver electrode method proved to be easy and useful in investigating the effects of ligands or other effectors on haemoglobin in solution.
Rezaei-Zarchi
S
S
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
Saboury
A A
AA
Ghourchian
H
H
Hong
J
J
Barzegar
A
A
Norouzi
P
P
Moosavi-Movahedi
A A
AA
Ganjali
M R
MR
Javed
A
A
D004563:Electrochemistry; D006454:Hemoglobins / Q000737:chemistry*; D006801:Humans; D010755:Organophosphates / Q000737:chemistry*; D010084:Oxidation-Reduction; D012834:Silver
D006454:Hemoglobins; D010755:Organophosphates; D012834:Silver