Evaluation of modified CMC and CMC-PVA as miscible polymer blend membranes for hepatocytes.
CMC and CMC-PVA were blended either with type I collagen, BSA or CS to obtain biocompatible membranes for evaluation as potential hepatocyte culture substrates. Pure and modified forms of CMC showed distinct surface, mechanical, and cell attachment properties. While the hydrophilicity decreased, the mechanical stability and the porosity of CMC membranes increased after blending. Serum proteins were adsorbed by all types of membranes. Among eight membranes tested, collagen-modified CMC was found to be a suitable membrane material for hepatocyte culture, in terms of mechanical and cell interaction properties.
Koç
Aysel
A
Ankara University, Faculty of Science and Biotechnology Institute, Tissue Engineering and Biomaterials Laboratory, Ankara 06100, Turkey.
Durkut
Serap
S
Elçin
A Eser
AE
Tan
Erdal
E
Elçin
Y Murat
YM
D000818:Animals; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry*; D018929:Cell Culture Techniques; D002470:Cell Survival; D002478:Cells, Cultured; D022781:Hepatocytes / Q000166:cytology* / Q000201:enzymology; D019164:Liver, Artificial; D008297:Male; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D051381:Rats; D044967:Serum; D017550:Spectroscopy, Fourier Transform Infrared; D014867:Water / Q000737:chemistry
D014867:Water; D011142:Polyvinyl Alcohol; D002266:Carboxymethylcellulose Sodium