title: Intragenomic diversity of the V1 regions of 16S rRNA genes in high-alkaline protease-producing Bacillus clausii spp.
abstract: Alkaliphilic Bacillus sp. strain KSM-K16, which produces high-alkaline M-protease, was characterized phenotypically, biochemically and genetically. This strain was identified as Bacillus clausii based on the results of taxonomic studies, including sequencing of the 16S rRNA gene and DNA-DNA hybridization. Seven rRNA operons in the genome were identified by pulsed-field gel electrophoresis. Sequencing of cloned 16S rRNA genes revealed two distinct types of variable region V1. Moreover, some cloned 16S rRNA genes in some of the reference strains of B. clausii had a V1 region of yet another type. The B. clausii strains could clearly be divided into at least two subgroups based on the frequencies of the types of cloned V1 sequence. Bacillus sp. strain KSM-K16 was found to be in a different phylogenetic position from other high-alkaline protease-producing strains of B. clausii.
authors: Tochigi Research Laboratories of Kao Corporation, 2606 Akabane, Ichikai, Haga, Tochigi 321-3497, Japan. kageyama.yasushi@kao.co.jp
authors: Yasushi
authors: Y
authors: Kageyama
authors: Yoshihiro
authors: Y
authors: Takaki
authors: Shigeru
authors: S
authors: Shimamura
authors: Shinro
authors: S
authors: Nishi
authors: Yuichi
authors: Y
authors: Nogi
authors: Kohsuke
authors: K
authors: Uchimura
authors: Tohru
authors: T
authors: Kobayashi
authors: Jun
authors: J
authors: Hitomi
authors: Katsuya
authors: K
authors: Ozaki
authors: Shuji
authors: S
authors: Kawai
authors: Susumu
authors: S
authors: Ito
authors: Koki
authors: K
authors: Horikoshi
mesh_terms: D001407:Bacillus / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis*; D004275:DNA, Ribosomal / Q000032:analysis*; D016521:Electrophoresis, Gel, Pulsed-Field; D014644:Genetic Variation; D005838:Genotype; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009876:Operon; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D021521:Ribotyping; D017422:Sequence Analysis, DNA; D012697:Serine Endopeptidases / Q000378:metabolism*; D013381:Subtilisins / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; C108281:M-protease; D012697:Serine Endopeptidases; D013381:Subtilisins; C032923:microbial serine proteinases