title: Yct1p, a novel, high-affinity, cysteine-specific transporter from the yeast Saccharomyces cerevisiae.
abstract: Cysteine transport in the yeast Saccharomyces cerevisiae is mediated by at least eight different permeases, none of which are specific for cysteine. We describe a novel, high-affinity, (K(m) = 55 microM), cysteine-specific transporter encoded by the ORF YLL055w that was initially identified by a combined strategy of data mining, bioinformatics, and genetic analysis. Null mutants of YLL055w, but not of the other genes encoding for transporters that mediate cysteine uptake such as GAP1, GNP1, MUP1, or AGP1 in a met15Delta background, resulted in a growth defect when cysteine, at low concentrations, was provided as the sole sulfur source. Transport experiments further revealed that Yll055wp was the major contributor to cysteine transport under these conditions. The contributions of the other transporters became relevant only at higher concentrations of cysteine or when YLL055w was either deleted or repressed. YLL055w expression was repressed by organic sulfur sources and was mediated by the Met4p-dependent sulfur regulatory network. The results reveal that YLL055w encodes the principal cysteine transporter in S. cerevisiae, which we have named YCT1 (yeast cysteine transporter). Interestingly, Yct1p belongs to the Dal5p family of transporters rather than the amino acid permease family to which all the known amino acid transporters belong.
authors: Institute of Microbial Technology, Sector 39-A, Chandigarh 160036, India.
authors: Jaspreet
authors: J
authors: Kaur
authors: Anand K
authors: AK
authors: Bachhawat
mesh_terms: D026921:Amino Acid Transport Systems, Neutral / Q000235:genetics* / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D001692:Biological Transport; D003001:Cloning, Molecular; D003545:Cysteine / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D017930:Genes, Reporter; D005838:Genotype; D007700:Kinetics; D016366:Open Reading Frames; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D026921:Amino Acid Transport Systems, Neutral; D000596:Amino Acids; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; C523288:Yct1 protein, S cerevisiae; D001616:beta-Galactosidase; D003545:Cysteine