Au nanoparticle network-type thin films formed via mixed assembling and cross-linking route for biosensor application: quartz crystal microbalance study.
We have investigated a means of producing Au nanoparticle network-type thin films that are derived by a one-step mixed-assembly cross-linking route, can avoid nonspecific interactions, and provide maximum binding to a specific target. Although a stepwise layer-by-layer construction method exists, our goal is to seek an alternative and simpler pathway for preparing thin films such as electrode nanomaterials. The model system consisting of Au nanoparticles, 1,6-hexananedithiol (HDT) cross-linkers, and 3-mercaptopropionic acid (MPA) captures was studied. The mixing of these three components in solutions allowed sequential assembly, cross-linking, and eventual precipitation of the network-type thin films on a Au substrate. Characterization of the films was carried out using UV-visible spectroscopy, cyclic voltammetry, and a quartz crystal microbalance. Such a thin film can be useful in biosensing and surface-coating applications. By controlling the ratio of HDT and MPA, we made Au nanoparticle network-type thin films on which goat-anti-human IgG antibodies that were immobilized can bind specifically to IgG with negligible nonspecific binding.
Shen
Guangyu
G
State Key Laboratory of Chem/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, People's Republic of China. glshen@hnu.cn
Wang
Hua
H
Shen
Guoli
G
Yu
Ruqin
R
D000818:Animals; D000906:Antibodies / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry; D007255:Information Services / Q000295:instrumentation; D008839:Microelectrodes; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D011485:Protein Binding; D011791:Quartz; D012680:Sensitivity and Specificity; D013499:Surface Properties
D000906:Antibodies; D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D004220:Disulfides; D007074:Immunoglobulin G; D011791:Quartz; D006046:Gold