Single-nucleotide polymorphisms in the IL-4 and IL-13 promoter region in aggressive periodontitis.
BACKGROUND
IL-4 and IL-13 polymorphisms have been shown to influence the susceptibility to systemic diseases. In this study, possible associations between the IL-4 -590 C-->T, IL-4 -34 C-->T, IL-13 -1112 C-->T and IL-13 -1512 A-->C promoter polymorphisms were investigated in subjects with generalized aggressive periodontitis (AgP) compared with healthy individuals.


METHODS
Fifty-eight patients with diagnosis of generalized AgP and 51 matched healthy controls participated in the study. Blood samples were collected and DNA isolated. Molecular analyses were performed by PCR-RFLP in a blind fashion. Genotype and allele frequencies among study groups were compared using Fisher's exact test (alpha value: 0.05). Pearson's chi(2) test was used for analysis of Hardy-Weinberg equilibrium.


RESULTS
The frequency of the IL-4 -590 T/T and IL-4 -34 T/T genotypes differed significantly between groups (p=0.05, 0.02, respectively), although the allele frequencies were similar. There was a higher frequency of the IL-4 -590 T/T and IL-4 -34 T/T genotypes in patients with AgP compared with controls. The genotype and allele frequencies of the IL-13 polymorphisms did not differ between groups.


CONCLUSIONS
This study demonstrated an association between the IL-4 -590 T/T and IL-4 -34 T/T genotypes and AgP. Further research is necessary to prove if there is an association of these polymorphisms with AgP, and if the polymorphisms have a functional effect.
Gonzales
J R
JR
Department of Periodontology, Justus-Liebig University, Giessen, Germany. jose.gonzales@dentist.med.uni-giessen.de
Mann
M
M
Stelzig
J
J
Bödeker
R H
RH
Meyle
J
J
D000225:Adenine; D000328:Adult; D016022:Case-Control Studies; D003596:Cytosine; D004247:DNA / Q000235:genetics; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D005884:Gingival Hemorrhage / Q000235:genetics / Q000276:immunology; D006720:Homozygote; D006801:Humans; D018793:Interleukin-13 / Q000235:genetics*; D015847:Interleukin-4 / Q000235:genetics*; D008297:Male; D017622:Periodontal Attachment Loss / Q000235:genetics / Q000276:immunology; D010514:Periodontal Pocket / Q000235:genetics / Q000276:immunology; D010518:Periodontitis / Q000235:genetics / Q000276:immunology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D016037:Single-Blind Method; D013941:Thymine
D018793:Interleukin-13; D015847:Interleukin-4; D003596:Cytosine; D004247:DNA; D000225:Adenine; D013941:Thymine