Identification and characterization of the human SOX6 promoter.
The present study attempted to identify and characterize the embryonic promoter of Sox6, a determinant regulator of chondrogenic differentiation. A common transcription start region for human and mouse Sox6 was initially identified, which contained a highly conserved sequence, A-box. Tandem repeats of A-box had a strong transcriptional activity both at the basal level and in response to Sox9. Cells carrying the 4xA-box-DsRed2 reporter fluoresced only upon chondrogenic differentiation. The 46-bp core enhancer region (CES6) was then identified in the 3' half of A-box, within which a C/EBP-binding motif was identified. Overexpressed C/EBPbeta activated the Sox6 promoter, and mutant 4xCES6 constructs lacking the C/EBP motif lost their basal activity. CES6 and nuclear extracts formed a specific complex, which was supershifted by anti-C/EBPbeta antibody, and in vitro translated C/EBPbeta specifically bound to CES6. Thus, we successfully identified the Sox6 promoter and its core enhancer and characterized the interactions with regulatory transcription factors.
Ikeda
Toshiyuki
T
Sensory and Motor System Medicine, Faculty of Medicine, University of Tokyo, Hongo 7-3-1, Bunkyo, Tokyo 113-8655, Japan.
Saito
Taku
T
Ushita
Masahiro
M
Yano
Fumiko
F
Kan
Akinori
A
Itaka
Keiji
K
Moro
Toru
T
Nakamura
Kozo
K
Kawaguchi
Hiroshi
H
Chung
Ung-Il
UI
D000818:Animals; D001483:Base Sequence; D020219:Chondrogenesis / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D006609:High Mobility Group Proteins / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D055755:SOXD Transcription Factors; D014157:Transcription Factors / Q000235:genetics*
D004268:DNA-Binding Proteins; D006609:High Mobility Group Proteins; C486270:SOX6 protein, human; D055755:SOXD Transcription Factors; D014157:Transcription Factors