title: Diagnosis of canine brucellosis by ELISA using an antigen obtained from wild Brucella canis.
abstract: An indirect ELISA test was developed for the diagnosis of Brucella canis infection in dogs. A bacterial whole cell extract was used as a solid phase antigen, using B. canis isolated from an infected animal. Sera from culture-positive and healthy negative animals were used as internal reference controls. The cut-off point was determined by a mathematical formula for a statistically valid value, which defined the upper prediction limit, based on the upper tail of the t-distribution of 21 negative control sera readings, for the confidence level of 99.5%. The sensitivity and specificity of the ELISA test were 95% and 91%, respectively. The ELISA test showed a significant concordance index (K=0.84) with the agar gel immunodiffusion test. The reliability of the ELISA for the detection of infected animals was established by a double blind study testing 280 sera provided by serum banks from different diagnostic and research institutions and analyzed by ROC Curve.
authors: Departamento de Patologia e Clínicas, Escola de Medicina Veterinária, Universidade Federal da Bahia, Av. Adhemar de Barros, 500 Salvador, BA, CEP 40170-110, Brazil. barrouin@ufba.br
authors: Stella Maria
authors: SM
authors: Barrouin-Melo
authors: Fernando Padilla
authors: FP
authors: Poester
authors: Marcos Borges
authors: MB
authors: Ribeiro
authors: Adriano Costa
authors: AC
authors: de Alcântara
authors: Paulo Henrique Palis
authors: PH
authors: Aguiar
authors: Ivana Lúcia
authors: IL
authors: Nascimento
authors: Robert Eduard
authors: RE
authors: Schaer
authors: Roberto Meyer
authors: RM
authors: Nascimento
authors: Songelí Menezes
authors: SM
authors: Freire
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D041002:Brucella canis / Q000276:immunology*; D002006:Brucellosis / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D004283:Dog Diseases / Q000097:blood / Q000175:diagnosis*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D012680:Sensitivity and Specificity
chemical_list: D000942:Antigens, Bacterial