A microfluidic system for saliva-based detection of infectious diseases.
A "lab-on-a-chip" system for detecting bacterial pathogens in oral fluid samples is described. The system comprises: (1) an oral fluid sample collector; (2) a disposable, plastic microfluidic cassette ("chip") for sample processing including immunochromatographic assay with a nitrocellulose lateral flow strip; (3) a platform that controls the cassette operation by providing metered quantities of reagents, temperature regulation, valve actuation; and (4) a laser scanner to interrogate the lateral flow strip. The microfluidic chip hosts a fluidic network for cell lysis, nucleic acid extraction and isolation, PCR, and labeling of the PCR product with bioconjugated, upconverting phosphor particles for detection on the lateral flow strip.
Chen
Zongyuan
Z
University of Pennsylvania School of Engineering and Applied Science, Philadelphia, Pennsylvania, USA.
Mauk
Michael G
MG
Wang
Jing
J
Abrams
William R
WR
Corstjens
Paul L A M
PL
Niedbala
R Sam
RS
Malamud
Daniel
D
Bau
Haim H
HH
D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D002478:Cells, Cultured; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D006801:Humans; D056656:Lab-On-A-Chip Devices; D044085:Microfluidics / Q000295:instrumentation; D016133:Polymerase Chain Reaction; D012463:Saliva / Q000382:microbiology
D004269:DNA, Bacterial