Surprising production of a new urdamycin derivative by S. fradiae Delta urdQ/R.
A strain (S. fradiae Delta urdQ/R) with mutations in urdQ and urdR encoding a dTDP-hexose-3,4-dehydratase and a dTDP-hexose-4-ketoreductase, respectively, produces a new urdamycin analogue (urdamycin X) with changes in the polyketide structure. The structure of urdamycin X has been elucidated by NMR spectroscopy. Urdamycin X was not detectable, even in small amounts, in either S. fradiae Delta urdQ, in S. fradiae DeltaurdR or in S. fradiae A0, a mutant lacking all glycosyltransferase genes. Complementation of S. fradiae Delta urdQ/R restored urdamycin A production indicating that the mutations did not cause any polar effect.
Fedoryshyn
M
M
Institut für Pharmazeutische Wissenschaften, Lehrstuhl für Pharmazeutische Biologie und Biotechnologie Albert-Ludwigs-Universität Freiburg, Stefan-Meier-Strasse 19, 79104 Freiburg, Germany.
Nur-e-Alam
M
M
Zhu
L
L
Luzhetskyy
A
A
Rohr
J
J
Bechthold
A
A
D000617:Aminoglycosides / Q000737:chemistry / Q000378:metabolism; D000880:Anthraquinones / Q000737:chemistry / Q000378:metabolism; D001709:Biotechnology; D015964:Gene Expression Regulation, Bacterial; D006031:Glycosylation; D018942:Macrolides / Q000737:chemistry / Q000378:metabolism; D009154:Mutation; D010957:Plasmids; D013302:Streptomyces / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D000095602:Angucyclines and Angucyclinones
D000617:Aminoglycosides; D000880:Anthraquinones; D018942:Macrolides; C051487:urdamycin B; C046608:kerriamycin B; D000095602:Angucyclines and Angucyclinones