Oxygen consumption of equine articular chondrocytes: Influence of applied oxygen tension and glucose concentration during culture.
We investigated the oxygen (O(2)) uptake of equine articular chondrocytes to assess their reactions to anoxia/re-oxygenation. They were cultured under 5% or 21% gas phase O(2) and at glucose concentrations of 0, 1.0 or 4.5g/L in the culture medium (n=3). Afterwards, the O(2) consumption rate of the chondrocytes was monitored (oxymetry) before and after an anoxia period of 25min. The glucose consumption and lactate release were measured at the end of the re-oxygenation period. The chondrocytes showed a minimal O(2) consumption rate, which was hardly changed by anoxia. Independently from the O(2) tension, glucose uptake by the cells was about 30% of the available culture medium glucose, thus higher for cells at 4.5g/L glucose (n=3). Lactate release was also independent from O(2) tension, but lower for cells at 4.5g/L glucose (n=3). Our observations indicated that O(2) consumption by equine chondrocytes was very low despite a functional mitochondrial respiratory chain, and nearly insensitive to anoxia/re-oxygenation. But the chondrocytes metabolism was modified by an excess of O(2) and glucose.
Schneider
Nicole
N
Centre of Oxygen Research & Development (CORD), Institut de Chimie, B6a, Sart Tilman, University of Liège, 4000 Liège, Belgium. ni.schneider@gmx.net
Mouithys-Mickalad
Ange
A
Lejeune
Jean-Philippe
JP
Duyckaerts
Claire
C
Sluse
Francis
F
Deby-Dupont
Ginette
G
Serteyn
Didier
D
D000464:Alginates / Q000378:metabolism; D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology* / Q000187:drug effects*; D002452:Cell Count; D018929:Cell Culture Techniques; D002469:Cell Separation; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects* / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology*; D020723:Glucuronic Acid / Q000378:metabolism; D006603:Hexuronic Acids / Q000378:metabolism; D006736:Horses; D019344:Lactic Acid / Q000096:biosynthesis; D008863:Microspheres; D010100:Oxygen / Q000494:pharmacology*; D010101:Oxygen Consumption / Q000187:drug effects*
D000464:Alginates; D006603:Hexuronic Acids; D019344:Lactic Acid; D020723:Glucuronic Acid; D003576:Electron Transport Complex IV; D005947:Glucose; D010100:Oxygen