Carbon fiber nanoelectrodes applied to microchip electrophoresis amperometric detection of neurotransmitter dopamine in rat pheochromocytoma (PC12) cells.
Microelectrodes have been adopted in electrochemical detection for CE or microchip CE in recent years. In this paper, the use of nanoelectrodes (with tip diameter of 100-300 nm) as the electrochemical detector in microchip CE is firstly reported. The experimental results indicated that both the sensitivity and resolution of microchip CE with the carbon fiber nanoelectrode (CFNE) amperometric detection have been improved markedly comparing with the traditional microelectrodes. The detection limit of dopamine (S/N = 3) is 5.9x10(-8) M, which is one or two orders of magnitude lower than that reported so far, and the resolution of dopamine (DA) and isoprenaline (IP) has also improved from 0.6 (using 7 mum carbon fiber microelectrodes, CFME) to 1.0. We assembled a novel and easily operated microchip CE system with end-column amperometric detection, which allows the convenient and fast replacement of the passivated electrodes. Under the optimized condition, the RSDs of peak height and migration time are 1.47 and 0.31%, respectively (n = 40), indicating that the system displays excellent reproducibility. The nanoelectrode-based microchip CE system has been successfully applied to the determination of DA in cultured rat pheochromocytoma (PC12) cells, and the average content of DA in an individual PC12 cell is 0.54 +/- 0.07 fmol, which is in good agreement with that reported in the literature.
Cheng
Han
H
Chemistry Department, Wuhan University, Wuhan, PR China.
Huang
Wei-Hua
WH
Chen
Rong-Sheng
RS
Wang
Zong-Li
ZL
Cheng
Jie-Ke
JK
D000818:Animals; D002244:Carbon / Q000145:classification; D000077482:Carbon Fiber; D002457:Cell Extracts; D003217:Conductometry / Q000379:methods; D004298:Dopamine / Q000032:analysis* / Q000737:chemistry; D046209:Electrophoresis, Microchip / Q000295:instrumentation* / Q000379:methods; D008832:Microchemistry / Q000295:instrumentation; D008839:Microelectrodes / Q000145:classification*; D008904:Miniaturization / Q000295:instrumentation; D037742:Nanotubes, Carbon / Q000145:classification*; D016716:PC12 Cells; D010673:Pheochromocytoma / Q000737:chemistry*; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000077482:Carbon Fiber; D002457:Cell Extracts; D037742:Nanotubes, Carbon; D002244:Carbon; D004298:Dopamine