Polymorphism of heart fatty acid-binding protein gene associatied with fatness traits in the chicken.
Fatty acid-binding proteins (FABP) belong to a superfamily of lipid binding proteins that exhibit a high affinity for long chain fatty acids and appear to function in metabolism and intracellular transportation of lipids. The current study was designed to investigate the effects of heart (H)-FABP gene on chicken growth and body composition traits. The Northeast Agricultural University divergent broiler lines for abdominal fat and a broiler X silkie F2 population were used in this study. Body weight and body composition traits were measured in the populations. Primers were designed according to the chicken H-FABP gene sequence. Polymorphisms between parental lines were detected by DNA sequencing. PCR-RFLP and PCR-fragment length polymorphism methods were developed to genotype the populations. The results showed that the H-FABP gene polymorphisms in the two populations were associated with abdominal fat percentage. It implied that H-FABP gene can be a candidate locus or linked to a major gene(s) that affects abdominal fat content in the chicken.
Wang
Qigui
Q
College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
Li
Hui
H
Leng
Li
L
Wang
Yuxiang
Y
Tang
Zhiquan
Z
Li
Ning
N
Zhang
Fuchun
F
D000273:Adipose Tissue / Q000254:growth & development* / Q000502:physiology; D000818:Animals; D001835:Body Weight / Q000235:genetics / Q000502:physiology; D002645:Chickens / Q000235:genetics* / Q000254:growth & development*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D050556:Fatty Acid-Binding Proteins / Q000235:genetics*; D005260:Female; D006239:Haplotypes / Q000235:genetics / Q000502:physiology; D016018:Least-Squares Analysis; D008297:Male; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012641:Selection, Genetic
D050556:Fatty Acid-Binding Proteins; D004247:DNA