title: Mesorhizobium spp. are the main microsymbionts of Caragana spp. grown in Liaoning Province of China.
abstract: Caragana species are woody legumes widely distributed in the arid regions of China. These plants form root nodules but their nodule bacteria have not been clearly classified. A total of 112 symbiotic bacterial isolates were obtained from four Caragana species grown in Liaoning Province and were characterized with ARDRA, sodium dodecyl sulfate-polyacrylamide gel electrophoresis of proteins, BOX-PCR and sequencing of the 16S rRNA gene. Most of them were classified as Mesorhizobium belonging to 11 putative species. Three isolates were identified as Rhizobium etli and Burkholderia spp. This study offers new information about the Caragana-rhizobia association and resource for selection of inoculants used in sustainable agriculture and for further studies on the Caragana rhizobia.
authors: Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture/Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing, China.
authors: Xue Rui
authors: XR
authors: Yan
authors: Wen Feng
authors: WF
authors: Chen
authors: Jun Fan
authors: JF
authors: Fu
authors: Yang Li
authors: YL
authors: Lu
authors: Cai Yun
authors: CY
authors: Xue
authors: Xin Hua
authors: XH
authors: Sui
authors: Ying
authors: Y
authors: Li
authors: En Tao
authors: ET
authors: Wang
authors: Wen Xin
authors: WX
authors: Chen
mesh_terms: D020561:Alphaproteobacteria / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D001426:Bacterial Proteins / Q000032:analysis; D032602:Caragana / Q000254:growth & development / Q000382:microbiology*; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013559:Symbiosis
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S