In vitro antimicrobial effect of encapsulated vancomycin on internalized Staphylococcus aureus within endothelial cells.
Vancomycin (VCN) is a glycopeptide antibiotic that is effective in the treatment of gram-positive bacterial infections, but mainly reserved for methicilin resistant Staphylococcus aureus. It is, however, ineffective against intracellular bacteria and hence a particulate form of VCN would be required. Bovine serum albumin (BSA) microspheres of VCN with a mean particle size of 5 +/- 1.6 microm were used. Human microvascular endothelial cells internalized both S. aureus and VCN microspheres in a time and concentration-dependent manner, however, the uptake was inhibited by cytochalasin D. Action of VCN on S. aureus in the intracellular microenvironment decreased the bacterial load considerably.
Nettey
Henry
H
Department of Pharmaceutical Sciences, Southern School of Pharmacy, Mercer University, Atlanta, GA, USA. hnettey@msn.com
Haswani
Dinesh
D
D'Souza
Martin
M
Oettinger
Carl
C
D000199:Actins / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D015638:Cytochalasin D / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers; D004339:Drug Compounding / Q000379:methods*; D004705:Endocytosis / Q000187:drug effects; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000382:microbiology; D005450:Fluorescamine / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D008863:Microspheres; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D010316:Particle Size; D011108:Polymers / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development; D014640:Vancomycin / Q000737:chemistry / Q000494:pharmacology*
D000199:Actins; D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D005456:Fluorescent Dyes; D019384:Nucleic Acid Synthesis Inhibitors; D011108:Polymers; D015638:Cytochalasin D; D012710:Serum Albumin, Bovine; D005450:Fluorescamine; D014640:Vancomycin