Preparative isolation and purification of geniposide from gardenia fruits by centrifugal partition chromatography.
The iridoid glycoside, geniposide was purified by centrifugal partition chromatography (CPC) with a two-phase solvent system composed of ethyl acetate:isopropanol:water (3:2:5, v/v) from an 80% methanolic extract of fruits of Gardenia jasminoides. Preparative CPC yielded 56.2 mg of geniposide in a one-step separation of 500 mg of extract, with a purity of 95% as determined by HPLC. Isolated geniposide was identified from its 1H-NMR, 13C-NMR and MS spectra.
Kim
Chul Young
CY
College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, Korea.
Kim
Jinwoong
J
D002498:Centrifugation; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D005638:Fruit / Q000737:chemistry*; D032062:Gardenia / Q000737:chemistry*; D007202:Indicators and Reagents; D039823:Iridoids / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D010936:Plant Extracts / Q000032:analysis; D011714:Pyrans / Q000302:isolation & purification*; D012997:Solvents; D021241:Spectrometry, Mass, Electrospray Ionization
D007202:Indicators and Reagents; D039823:Iridoids; D010936:Plant Extracts; D011714:Pyrans; D012997:Solvents; C007835:geniposide