The Na+/Ca2+ exchanger isoform 3 (NCX3) but not isoform 2 (NCX2) and 1 (NCX1) singly transfected in BHK cells plays a protective role in a model of in vitro hypoxia.
Chemical hypoxia produces depletion of ATP, intracellular Ca2+ overload, and cell death. The role of Na+/Ca2+ exchanger (NCX), the major plasma membrane Ca2+ extruding system, has been explored in chemical hypoxia using BHK cells stably transfected with the three mammalian NCX isoforms: NCX1, NCX2, and NCX3. Here we report that the three isoforms show similar activity evaluated as [Ca2+]i increase evoked by Na+-free medium exposure in Fura-2-loaded single cells and NCX3 transfected cells are less vulnerable to chemical hypoxia compared to NCX1- and NCX2-transfected cells, suggesting that NCX3 could play a more relevant protective role during chemical hypoxia.
Secondo
Agnese
A
Division of Pharmacology, Department of Neuroscience, School of Medicine, Federico II University of Naples, Via Pansini 5, 80131 Naples, Italy.
Staiano
Ilaria Rosaria
IR
Scorziello
Antonella
A
Sirabella
Rossana
R
Boscia
Francesca
F
Adornetto
Annagrazia
A
Canzoniero
Lorella Maria Teresa
LM
Di Renzo
Gianfranco
G
Annunziato
Lucio
L
D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D000860:Hypoxia / Q000503:physiopathology*; D066298:In Vitro Techniques; D020033:Protein Isoforms / Q000235:genetics / Q000502:physiology*; D019831:Sodium-Calcium Exchanger / Q000235:genetics / Q000502:physiology*; D014162:Transfection
D020033:Protein Isoforms; D019831:Sodium-Calcium Exchanger