Acclimation to singlet oxygen stress in Chlamydomonas reinhardtii.
In an aerobic environment, responding to oxidative cues is critical for physiological adaptation (acclimation) to changing environmental conditions. The unicellular alga Chlamydomonas reinhardtii was tested for the ability to acclimate to specific forms of oxidative stress. Acclimation was defined as the ability of a sublethal pretreatment with a reactive oxygen species to activate defense responses that subsequently enhance survival of that stress. C. reinhardtii exhibited a strong acclimation response to rose bengal, a photosensitizing dye that produces singlet oxygen. This acclimation was dependent upon photosensitization and occurred only when pretreatment was administered in the light. Shifting cells from low light to high light also enhanced resistance to singlet oxygen, suggesting an overlap in high-light and singlet oxygen response pathways. Microarray analysis of RNA levels indicated that a relatively small number of genes respond to sublethal levels of singlet oxygen. Constitutive overexpression of either of two such genes, a glutathione peroxidase gene and a glutathione S-transferase gene, was sufficient to enhance singlet oxygen resistance. Escherichia coli and Saccharomyces cerevisiae exhibit well-defined responses to reactive oxygen but did not acclimate to singlet oxygen, possibly reflecting the relative importance of singlet oxygen stress for photosynthetic organisms.
Ledford
Heidi K
HK
Department of Plant and Microbial Biology, 111 Koshland Hall, University of California-Berkeley, Berkeley, CA 94720-3102, USA.
Chin
Brian L
BL
Niyogi
Krishna K
KK
D000222:Adaptation, Physiological; D000818:Animals; D002338:Carotenoids / Q000378:metabolism; D016825:Chlamydomonas reinhardtii / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D008027:Light; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress; D017319:Photosensitizing Agents / Q000378:metabolism; D012395:Rose Bengal / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D026082:Singlet Oxygen / Q000378:metabolism*; D014810:Vitamin E / Q000378:metabolism
D005456:Fluorescent Dyes; D017319:Photosensitizing Agents; D014810:Vitamin E; D026082:Singlet Oxygen; D012395:Rose Bengal; D002338:Carotenoids; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase