title: Quantitative proteomic assessment of very early cellular signaling events.
abstract: Technical limitations have prevented proteomic analyses of events occurring less than 30 s after signal initiation. We developed an automated, continuous quench-flow system allowing quantitative proteomic assessment of very early cellular signaling events (qPACE) with a time resolution of 1 s. Using this technique, we determined that autophosphorylation of the epidermal growth factor receptor occurs within 1 s after ligand stimulation and is followed rapidly by phosphorylation of the downstream signaling intermediates Src homologous and collagen-like protein and phospholipase C gamma 1.
authors: Center of Experimental BioInformatics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Campusvej 55, DK-5230, Odense, Denmark.
authors: Joern
authors: J
authors: Dengjel
authors: Vyacheslav
authors: V
authors: Akimov
authors: Jesper V
authors: JV
authors: Olsen
authors: Jakob
authors: J
authors: Bunkenborg
authors: Matthias
authors: M
authors: Mann
authors: Blagoy
authors: B
authors: Blagoev
authors: Jens S
authors: JS
authors: Andersen
mesh_terms: D066246:ErbB Receptors / Q000378:metabolism*; D036681:Fluorescence Recovery After Photobleaching / Q000379:methods*; D008657:Metabolic Clearance Rate; D008856:Microscopy, Fluorescence / Q000379:methods*; D010766:Phosphorylation; D020543:Proteome / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D013997:Time Factors
chemical_list: D020543:Proteome; D066246:ErbB Receptors