Label-free detection of Babesia bovis infected red blood cells using impedance spectroscopy on a microfabricated flow cytometer.
Impedance spectroscopy is a powerful tool for label-free analysis and characterisation of living cells. In this work, we achieved the detection of Babesia bovis infected red blood cells using impedance spectroscopy on a microfabricated flow cytometer. The cellular modifications caused by the intracellular parasite result in a shift in impedance which can be measured dielectrically. Thus, a rapid cell-by-cell detection with microliter amounts of reagents is possible. Unlike other diagnostic tests, this method does not depend on extensive sample pre-treatment or expensive chemicals and equipment.
Küttel
Claudia
C
Laboratory of Microsystems, Ecole Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland. kuettel@nano.mavt.ethz.ch
Nascimento
Elisabete
E
Demierre
Nicolas
N
Silva
Tiago
T
Braschler
Thomas
T
Renaud
Philippe
P
Oliva
Abel G
AG
D000818:Animals; D016793:Babesia bovis / Q000302:isolation & purification*; D001404:Babesiosis / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000469:parasitology; D017097:Electric Impedance; D004912:Erythrocytes / Q000469:parasitology*; D005434:Flow Cytometry / Q000295:instrumentation*; D010274:Parasitology / Q000379:methods; D013057:Spectrum Analysis / Q000379:methods*