title: Expression of SPAM1 (PH-20) in the murine kidney is not accompanied by hyaluronidase activity: evidence for potential roles in fluid and water reabsorption.
abstract: BACKGROUND
  A role for Sperm Adhesion Molecule 1 (SPAM1) hyaluronidase in murine kidney, where Spam1 transcript levels have been reported to be higher in males, has not been clarified.
  
  
  METHODS
  Spam1 RNA and protein were studied using RT-PCR, in situhybridization, Western blotting, immunohistochemistry and hyaluronic acid substrate gel electrophoresis. Urine volume and osmolality were studied in wild-type and Spam1 null mice.
  
  
  RESULTS
  While RT-PCR supported a tendency of higher RNA expression in males, no sex difference for the protein was detectable in the cortex, medulla, and urine. Transcripts were predominantly localized in the proximal tubules and glomeruli, with lower levels in the medulla. Similarly, Western blotting and immunohistochemistry revealed that SPAM1 is more abundant in the cortex. Hyaluronidase activity was absent at neutral and acidic pH: suggesting non-enzymatic role(s) for SPAM1. Wild-type and Spam1 null mice given free access to water showed significantly reduced urine volumes (p < 0.01; n = 12) in the latter. Baseline urine osmolality was similar in both, leading to a significantly (p < 0.05) lower osmolar output in the nulls. After water deprivation (24 h), a significant (p < 0.01) increase in urine osmolality was seen only for wild-type mice.
  
  
  CONCLUSIONS
  SPAM1 is implicated in fluid reabsorption and urine concentration.
authors: Department of Biological Sciences, University of Delaware, Newark, NJ 19716, USA.
authors: Anastasia
authors: A
authors: Grigorieva
authors: Genevieve S
authors: GS
authors: Griffiths
authors: Hong
authors: H
authors: Zhang
authors: Gary
authors: G
authors: Laverty
authors: Minghai
authors: M
authors: Shao
authors: Leslie
authors: L
authors: Taylor
authors: Patricia A
authors: PA
authors: Martin-DeLeon
mesh_terms: D000818:Animals; D021461:Animals, Outbred Strains; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000378:metabolism*; D004326:Drinking / Q000502:physiology; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D006821:Hyaluronoglucosaminidase / Q000235:genetics* / Q000378:metabolism*; D007150:Immunohistochemistry; D007668:Kidney / Q000201:enzymology*; D007672:Kidney Cortex / Q000201:enzymology; D007678:Kidney Glomerulus / Q000201:enzymology; D007687:Kidney Tubules, Proximal / Q000201:enzymology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D018345:Mice, Knockout; D009994:Osmolar Concentration; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014556:Urine; D019459:Urothelium / Q000201:enzymology; D014867:Water / Q000378:metabolism; D014882:Water-Electrolyte Balance / Q000502:physiology*
chemical_list: D015815:Cell Adhesion Molecules; D012333:RNA, Messenger; D014867:Water; D006821:Hyaluronoglucosaminidase; C066852:hyaluronidase PH-20