Multicopy blaOXA-58 gene as a source of high-level resistance to carbapenems in Acinetobacter baumannii.
The mechanisms at the origin of heterogeneous carbapenem resistance levels observed among Acinetobacter baumannii isolates collected in 2005 in a large University Hospital of Rome, Italy, were investigated. These isolates were related and possessed similar plasmids carrying the carbapenem-hydrolyzing oxacillinase gene bla(OXA-58) but showed variable levels of resistance to carbapenems. Analysis of sequences surrounding the bla(OXA-58) gene showed genetic variability, with the presence in several isolates of multiple copies of the bla(OXA-58) gene; this extra copy number was likely related to an IS26-mediated transposition or recombination process.
Bertini
Alessia
A
Department of Infectious, Parasitic, Immune-Mediated Diseases, Istituto Superiore di Sanitá, Rome, Italy.
Poirel
Laurent
L
Bernabeu
Sandrine
S
Fortini
Daniela
D
Villa
Laura
L
Nordmann
Patrice
P
Carattoli
Alessandra
A
D040981:Acinetobacter baumannii / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001483:Base Sequence; D015780:Carbapenems / Q000494:pharmacology*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D018628:Gene Dosage; D005798:Genes, Bacterial; D014644:Genetic Variation; D006801:Humans; D008826:Microbial Sensitivity Tests; D021141:Nucleic Acid Amplification Techniques; D010957:Plasmids; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics
D000900:Anti-Bacterial Agents; D015780:Carbapenems; D004269:DNA, Bacterial; D001618:beta-Lactamases; C108449:oxacillinase