title: Calcium loss from root canal dentin following EDTA, EGTA, EDTAC, and tetracycline-HCl treatment with or without subsequent NaOCl irrigation.
abstract: The aim of this study was to determine the extent of calcium removal on root canal dentin after 17% EDTA, 17% EGTA, 15% EDTAC, and 1% tetracycline-HCl treatment; with or without subsequent use of 2.5% NaOCl. Extracted single-rooted human teeth were bisected longitudinally and the root halves (n=100) were isolated with nail varnish, leaving the root canal exposed. The samples were immersed in the test solutions for 1 and 5 minutes, after which the amount of calcium ion (Ca(2+)) release into the solutions was determined by flame photometry. Regardless of treatment time, all single (treatment solution only) and combined (treatment solution with subsequent NaOCl application) irrigation regimens removed significantly more Ca(2+) than control treatment (distilled water). Compared with other groups, treatment with 17% EDTA and 17% EDTA + 2.5% NaOCl resulted in the maximum amount of Ca(2+) removal from root canal dentin (p<0.05). All combined-treatment groups except 17% EGTA + 2.5% NaOCl removed significantly more Ca(2+) than their single-treatment versions (p<0.05). Within each test group, extending the treatment time to 5 minutes resulted in significantly more Ca(2+) removal (p<0.05).
authors: Department of Endodontics, Faculty of Dentistry, Hacettepe University, Ankara, Turkey. cemsayin@yahoo.com
authors: T Cem
authors: TC
authors: Sayin
authors: Ahmet
authors: A
authors: Serper
authors: Zafer C
authors: ZC
authors: Cehreli
authors: Sukru
authors: S
authors: Kalayci
mesh_terms: D002118:Calcium; D002614:Chelating Agents / Q000009:adverse effects*; D003786:Dental Pulp Cavity / Q000737:chemistry / Q000187:drug effects*; D003804:Dentin / Q000737:chemistry / Q000187:drug effects*; D004338:Drug Combinations; D004492:Edetic Acid / Q000009:adverse effects; D004533:Egtazic Acid / Q000009:adverse effects; D006801:Humans; D012388:Root Canal Irrigants / Q000009:adverse effects*; D018709:Statistics, Nonparametric; D013752:Tetracycline / Q000009:adverse effects
chemical_list: D002614:Chelating Agents; D004338:Drug Combinations; D012388:Root Canal Irrigants; D004533:Egtazic Acid; D004492:Edetic Acid; D013752:Tetracycline; D002118:Calcium