CNS midline cells influence the division and survival of lateral glia in the Drosophila nervous system.
Central nervous system (CNS) midline cells are essential for identity determination and differentiation of neurons in the Drosophila nervous system. It is not clear, however, whether CNS midline cells are also involved in the development of lateral glial cells. The roles of CNS midline cells in lateral glia development were elucidated using general markers for lateral glia, such as glial cell missing and reverse polarity, and specific enhancer trap lines labeling the longitudinal, A, B, medial cell body, peripheral, and exit glia. We found that CNS midline cells were necessary for the proper expression of glial cell missing, reverse polarity, and other lateral glia markers only during the later stages of development, suggesting that they are not required for initial identity determination. Instead, CNS midline cells appear to be necessary for proper division and survival of lateral glia. CNS midline cells were also required for proper positioning of three exit glia at the junction of segmental and intersegmental nerves, as well as some peripheral glia along motor and sensory axon pathways. This study demonstrated that CNS midline cells are extrinsically required for the proper division, migration, and survival of various classes of lateral glia from the ventral neuroectoderm.
Kim
So Hee
SH
Department of Chemistry, Konkuk University, Seoul, Korea.
Kim
Hyeon Ju
HJ
Kim
Soo Youn
SY
Jeon
Sang-Hak
SH
Kim
Sang Hee
SH
D000818:Animals; D030801:Animals, Genetically Modified; D002455:Cell Division; D002465:Cell Movement; D002470:Cell Survival; D002490:Central Nervous System / Q000166:cytology* / Q000196:embryology* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics; D004330:Drosophila / Q000166:cytology* / Q000196:embryology* / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D018398:Homeodomain Proteins / Q000235:genetics; D017403:In Situ Hybridization; D007763:Lac Operon; D008297:Male; D009154:Mutation; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C102805:GCM protein, Drosophila; D018398:Homeodomain Proteins; D014157:Transcription Factors; C089972:repo protein, Drosophila