In situ hybridization for Lawsonia intracellularis--specific 16s rRNA sequence in paraffin-embedded tissue using a digoxigenin-labeled oligonucleotide probe.
An in situ hybridization (ISH) procedure with a digoxigenin-labeled oligonucleotide probe for detection of Lawsonia intracellularis in paraffin-embedded tissue is described. This technique recognized 71% of PCR-positive cases and was thus superior to Warthin-Starry silver stain, which only detected 41%. The presented ISH is of comparable sensitivity to previously published immunohistochemical assays and is recommended for laboratories wishing to diagnose L. intracellularis infections in tissue sections but without access to antibodies.
Weissenböck
Herbert
H
Institute of Pathology and Forensic Veterinary Medicine, Department of Pathobiology, Veterinärplatz 1, A-1210 Vienna, Austria.
Mrakovcic
Maria
M
Ladinig
Andrea
A
Fragner
Karin
K
D000818:Animals; D004269:DNA, Bacterial / Q000235:genetics; D045824:Desulfovibrionaceae Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D004076:Digoxigenin; D017403:In Situ Hybridization / Q000379:methods / Q000662:veterinary*; D007410:Intestinal Diseases / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D020639:Lawsonia Bacteria / Q000235:genetics / Q000302:isolation & purification*; D015345:Oligonucleotide Probes / Q000235:genetics*; D016612:Paraffin Embedding; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000382:microbiology*
D004269:DNA, Bacterial; D015345:Oligonucleotide Probes; D012336:RNA, Ribosomal, 16S; D004076:Digoxigenin