Actin-related protein Arp4 functions in kinetochore assembly.
The actin-related proteins (Arps) comprise a conserved protein family. Arp4p is found in large multisubunits of the INO80 and SWR1 chromatin remodeling complexes and in the NuA4 histone acetyltransferase complex. Here we show that arp4 (arp4S23A/D159A) temperature-sensitive cells are defective in G2/M phase function. arp4 mutants are sensitive to the microtubule depolymerizing agent benomyl and arrest at G2/M phase at restrictive temperature. Arp4p is associated with centromeric and telomeric regions throughout cell cycle. Ino80p, Esa1p and Swr1p, components of the INO80, NuA4 and SWR1 complexes, respectively, also associate with centromeres. The association of many kinetochore components including Cse4p, a component of the centromere nucleosome, Mtw1p and Ctf3p is partially impaired in arp4 cells, suggesting that the G2/M arrest of arp4 mutant cells is due to a defect in formation of the chromosomal segregation apparatus.
Ogiwara
Hideaki
H
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6-3, Aramaki, Aoba-ku, Sendai, Miyagi 980-8578, Japan.
Ui
Ayako
A
Kawashima
Satoshi
S
Kugou
Kazuto
K
Onoda
Fumitoshi
F
Iwahashi
Hitoshi
H
Harata
Masahiko
M
Ohta
Kunihiro
K
Enomoto
Takemi
T
Seki
Masayuki
M
D000199:Actins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D001665:Binding Sites; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division; D016195:G2 Phase; D023281:Genomics; D018386:Kinetochores / Q000378:metabolism*; D009154:Mutation; D015739:Nocodazole / Q000494:pharmacology; D009687:Nuclear Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*
D000199:Actins; C088827:Arp4 protein, S cerevisiae; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins; D015739:Nocodazole