title: Frequency and phenotypic implications of mitochondrial DNA mutations in human squamous cell cancers of the head and neck.
abstract: Mitochondrial genomic mutations are found in a variety of human cancers; however, the frequency of mitochondrial DNA (mtDNA) mutations in coding regions remains poorly defined, and the functional effects of mitochondrial mutations found in primary human cancers are not well described. Using MitoChip, we sequenced the whole mitochondrial genome in 83 head and neck squamous cell carcinomas. Forty-one of 83 (49%) tumors contained mtDNA mutations. Mutations occurred within noncoding (D-loop) and coding regions. A nonrandom distribution of mutations was found throughout the mitochondrial enzyme complex components. Sequencing of margins with dysplasia demonstrated an identical nonconservative mitochondrial mutation (A76T in ND4L) as the tumor, suggesting a role of mtDNA mutation in tumor progression. Analysis of p53 status showed that mtDNA mutations correlated positively with p53 mutations (P < 0.002). To characterize biological function of the mtDNA mutations, we cloned NADH dehydrogenase subunit 2 (ND2) mutants based on primary tumor mutations. Expression of the nuclear-transcribed, mitochondrial-targeted ND2 mutants resulted in increased anchorage-dependent and -independent growth, which was accompanied by increased reactive oxygen species production and an aerobic glycolytic metabolic phenotype with hypoxia-inducible factor (HIF)-1alpha induction that is reversible by ascorbate. Cancer-specific mitochondrial mutations may contribute to development of a malignant phenotype by direct genotoxic effects from increased reactive oxygen species production as well as induction of aerobic glycolysis and growth promotion.
authors: Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins Medical Institutions, Baltimore, MD 21287, USA.
authors: Shaoyu
authors: S
authors: Zhou
authors: Sushant
authors: S
authors: Kachhap
authors: Wenyue
authors: W
authors: Sun
authors: Guojun
authors: G
authors: Wu
authors: Alice
authors: A
authors: Chuang
authors: Luana
authors: L
authors: Poeta
authors: Lawson
authors: L
authors: Grumbine
authors: Suhail K
authors: SK
authors: Mithani
authors: Aditi
authors: A
authors: Chatterjee
authors: Wayne
authors: W
authors: Koch
authors: William H
authors: WH
authors: Westra
authors: Anirban
authors: A
authors: Maitra
authors: Chad
authors: C
authors: Glazer
authors: Michael
authors: M
authors: Carducci
authors: David
authors: D
authors: Sidransky
authors: Thomas
authors: T
authors: McFate
authors: Ajay
authors: A
authors: Verma
authors: Joseph A
authors: JA
authors: Califano
mesh_terms: D004272:DNA, Mitochondrial / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000096:biosynthesis; D019344:Lactic Acid / Q000096:biosynthesis; D009154:Mutation / Q000235:genetics; D018307:Neoplasms, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D010641:Phenotype; D011773:Pyruvates / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
chemical_list: D004272:DNA, Mitochondrial; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D011773:Pyruvates; D017382:Reactive Oxygen Species; D016159:Tumor Suppressor Protein p53; D019344:Lactic Acid