title: IL-13 induces expression of CD36 in human monocytes through PPARgamma activation.
abstract: The class B scavenger receptor CD36 is a component of the pattern recognition receptors on monocytes that recognizes a variety of molecules. CD36 expression in monocytes depends on exposure to soluble mediators. We demonstrate here that CD36 expression is induced in human monocytes following exposure to IL-13, a Th2 cytokine, via the peroxisome proliferator-activated receptor (PPAR)gamma pathway. Induction of CD36 protein was paralleled by an increase in CD36 mRNA. The PPARgamma pathway was demonstrated using transfection of a PPARgamma expression plasmid into the murine macrophage cell line RAW264.7, expressing very low levels of PPARgamma, and in peritoneal macrophages from PPARgamma-conditional null mice. We also show that CD36 induction by IL-13 via PPARgamma is dependent on phospholipase A2 activation and that IL-13 induces the production of endogenous 15-deoxy-Delta12,14-prostaglandin J2, an endogenous PPARgamma ligand, and its nuclear localization in human monocytes. Finally, we demonstrate that CD36 and PPARgamma are involved in IL-13-mediated phagocytosis of Plasmodium falciparum-parasitized erythrocytes. These results reveal a novel role for PPARgamma in the alternative activation of monocytes by IL-13, suggesting that endogenous PPARgamma ligands, produced by phospholipase A2 activation, could contribute to the biochemical and cellular functions of CD36.
authors: Macrophages, Mediateurs de l'Inflammation et Interactions Cellulaires, Université Paul Sabatier Toulouse III, INSERM IFR 31, Toulouse, France. berry.a@chu-toulouse.fr
authors: Antoine
authors: A
authors: Berry
authors: Patricia
authors: P
authors: Balard
authors: Agnès
authors: A
authors: Coste
authors: David
authors: D
authors: Olagnier
authors: Céline
authors: C
authors: Lagane
authors: Hélène
authors: H
authors: Authier
authors: Françoise
authors: F
authors: Benoit-Vical
authors: Jean-Claude
authors: JC
authors: Lepert
authors: Jean-Paul
authors: JP
authors: Séguéla
authors: Jean-François
authors: JF
authors: Magnaval
authors: Pierre
authors: P
authors: Chambon
authors: Daniel
authors: D
authors: Metzger
authors: Béatrice
authors: B
authors: Desvergne
authors: Walter
authors: W
authors: Wahli
authors: Johan
authors: J
authors: Auwerx
authors: Bernard
authors: B
authors: Pipy
mesh_terms: D000813:Anilides / Q000494:pharmacology; D000818:Animals; D001095:Arachidonic Acids / Q000494:pharmacology; D018955:CD36 Antigens / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D004247:DNA / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects / Q000469:parasitology; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007004:Hypoglycemic Agents / Q000494:pharmacology; D018793:Interleukin-13 / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D063065:Organophosphonates / Q000494:pharmacology; D047495:PPAR gamma / Q000819:agonists / Q000037:antagonists & inhibitors / Q000502:physiology*; D010587:Phagocytosis / Q000187:drug effects; D010741:Phospholipases A / Q000037:antagonists & inhibitors; D054467:Phospholipases A2; D010963:Plasmodium falciparum / Q000502:physiology; D015230:Prostaglandin D2 / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D011485:Protein Binding / Q000187:drug effects; D000077154:Rosiglitazone; D045162:Thiazolidinediones / Q000494:pharmacology; D014162:Transfection
chemical_list: C477819:15-deoxyprostaglandin J2; C457499:2-chloro-5-nitrobenzanilide; D000813:Anilides; D001095:Arachidonic Acids; D018955:CD36 Antigens; D004791:Enzyme Inhibitors; D007004:Hypoglycemic Agents; D018793:Interleukin-13; D063065:Organophosphonates; D047495:PPAR gamma; D045162:Thiazolidinediones; C104810:methyl arachidonylfluorophosphonate; D000077154:Rosiglitazone; D004247:DNA; D010741:Phospholipases A; D054467:Phospholipases A2; D015230:Prostaglandin D2