[Effect of adenovirus-mediated mda-7/IL-24 gene infection on apoptosis of drug-resistant human ovarian cancer cell lines OVCAR-3 and OVCAR-8/TR].
OBJECTIVE
Melanoma differentiation associated gene-7 (mda-7/IL-24) has double functions: specifically induces tumor cell apoptosis and modulates immune responses. Therefore, it is a strong candidate for human cancer gene therapy. This study was to evaluate the effect of adenovirus-mediated mda-7/IL-24 infection on the apoptosis of drug-resistant ovarian cancer cell lines OVCAR-3 and OVCAR-8/TR.


METHODS
Adenovirus-mediated mda-7/IL-24 (Ad.mda-7/IL-24) was constructed using AdEasy 1 system. OVCAR-3 and OVCAR-8/TR cells were infected by Ad.mda-7/IL-24. The expression of MDA-7/IL-24 protein was detected by Western blot. Cell apoptosis was detected by flow cytometry with Hoechst33258 staining. Cell cycle distribution was detected by flow cytometry.


RESULTS
The recombinant Ad.mda-7/IL-24 was confirmed by DNA sequencing and electrophoresis. The expression of MDA-7/IL-24 protein was detected in the cells after infection. Within 72 h after Ad.mda-7/IL-24 infection, the maximal apoptosis rates of OVCAR-3 and OVCAR-8/TR cells were 14.1% and 32.4%, respectively, significantly higher than empty vector group and uninfected group.


CONCLUSIONS
The recombinant Ad.mda-7/IL-24 was successfully constructed. It can induce apoptosis in drug-resistant ovarian cancer OVCAR-3 and OVCAR-8/TR cells.
Xiong
Ju
J
Department of Gynecology and Obstetrics, The Second West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, PR China.
Peng
Zhi-Lan
ZL
Tan
Xin
X
Yan
Nai-Hong
NH
D000256:Adenoviridae / Q000235:genetics*; D017209:Apoptosis; D015153:Blotting, Western; D002453:Cell Cycle; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm; D005260:Female; D005434:Flow Cytometry; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D007378:Interleukins / Q000235:genetics / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D000099180:Interleukin-24
D007378:Interleukins; D011994:Recombinant Proteins; D000099180:Interleukin-24