Use of the capillary-cumulus oophorus model for evaluating the selection of spermatozoa.
OBJECTIVE
To establish the action of the cumulus mass on the function of human spermatozoa.


METHODS
A prospective, controlled clinical study.


METHODS
Academic infertility clinic.


METHODS
Semen donors and IVF patients.


METHODS
Donor human cumulus oophorus complex was aspirated into a glass Pasteur pipette. The semen of selected IVF patients was then loaded into the pipette, and the spermatozoa were allowed to transverse the cumulus matrix. The penetrated spermatozoa were then collected and underwent sperm functional tests, namely chlortetracycline, fluorescein isothiocyanate-labeled Pisum sativum, and chromomycin A(3). These were then compared with the fertilization rates of the IVF patients used in the study.


METHODS
The cumulus oophorus' action on capacitational processes of human spermatozoa.


RESULTS
The penetrated spermatozoa showed a higher tendency to be capacitated and acrosome-reacted and to have superior chromosomal packaging quality, compared with the initial semen sample.


CONCLUSIONS
Cumulus oophorus cells play a vital role in the fertilization process, especially during the capacitational stages. This method could physiologically select spermatozoa to be used in assisted reproductive techniques, especially intracytoplasmic sperm injection, in which sperm usually are immobile (because of low motility or because spermatozoa were testicularly retrieved).
Rijsdijk
Michelle
M
Fertility and Wellness Centre, St. Georges Medical Suites, Port Elizabeth, Republic of South Africa. mrijsdijk@telkomsa.net
Franken
Daniel Rossouw
DR
D020101:Acrosome Reaction / Q000502:physiology; D002197:Capillary Action; D002469:Cell Separation; D002843:Chromatin / Q000737:chemistry; D054885:Cumulus Cells / Q000166:cytology* / Q000502:physiology; D005260:Female; D019793:Fluorescein / Q000032:analysis; D006801:Humans; D008297:Male; D008954:Models, Biological; D011247:Pregnancy; D018873:Pregnancy Rate; D027724:Reproductive Techniques, Assisted / Q000295:instrumentation*; D013075:Sperm Capacitation / Q000502:physiology; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000166:cytology* / Q000502:physiology*
D002843:Chromatin; D019793:Fluorescein