Layered peptide arrays: a diverse technique for antibody screening of clinical samples.
The layered peptide array (LPA) is a recently developed technique designed to measure antibody levels in a multiplex, high-throughput manner. LPAs can assess antibody presence either in fluid samples or from tissues while maintaining the two-dimensional orientation of the life science platform. In this manuscript, we evaluated and assessed the performance of the LPA platform, focusing on throughput capability, sensitivity, and specificity of the assay in several different systems.
Gannot
Gallya
G
Pathogenetics Unit, Laboratory of Pathology and Urologic Oncology Branch, Surgery Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-4605, USA.
Tangrea
Michael A
MA
Chuaqui
Rodrigo F
RF
Gillespie
John W
JW
Emmert-Buck
Michael R
MR
D000906:Antibodies / Q000032:analysis* / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007150:Immunohistochemistry; D010455:Peptides / Q000032:analysis* / Q000097:blood; D040081:Protein Array Analysis
D000906:Antibodies; D010455:Peptides