F-actin binding region of SPIN90 C-terminus is essential for actin polymerization and lamellipodia formation.
We recently reported that SPIN90 is able to bind with several proteins involved in regulating actin cytoskeleton networks, including dynamin, WASP, beta PIX, and Nck. Based on these findings, we investigated how SPIN90 regulates the actin cytoskeleton and promotes actin assembly. This study demonstrated that aluminium fluoride-induced localization of SPIN90 to lamellipodia requires amino acids 582-722 at the SPIN90 C-terminus, which is also essential for F-actin binding and Arp2/3 complex mediated polymerization of actin into branched actin filaments. Furthermore, after deletion of the F-actin binding region (582-722 SPIN90) failed to localize at the membrane edge and was unable to promote lamellipodia formation, suggesting that the F-actin binding region in the SPIN90 C-terminus is essential for the formation of branched actin networks and regulation of the actin cytoskeleton at the leading edge of cells.
Kim
Dae Joong
DJ
Department of Life Science and Center for Distributed Sensor Network, Gwangju Institute of Science and Technology, Gwangju, Korea.
Kim
Sung Hyun
SH
Kim
Seon-Myung
SM
Bae
Jeom Il
JI
Ahnn
Joohong
J
Song
Woo Keun
WK
D008841:Actin Cytoskeleton / Q000378:metabolism; D051376:Actin-Related Protein 2-3 Complex / Q000378:metabolism; D000199:Actins / Q000737:chemistry* / Q000378:metabolism*; D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry* / Q000378:metabolism*; D017607:Aluminum Compounds / Q000494:pharmacology; D045744:Cell Line, Tumor; D005459:Fluorides / Q000494:pharmacology; D006801:Humans; D008954:Models, Biological; D011485:Protein Binding / Q000187:drug effects; D021381:Protein Transport / Q000187:drug effects; D011554:Pseudopodia / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism
D051376:Actin-Related Protein 2-3 Complex; D000199:Actins; D048868:Adaptor Proteins, Signal Transducing; D017607:Aluminum Compounds; D005459:Fluorides; C032311:aluminum fluoride