title: Increased inflammatory factors activity in model rats with experimental autoimmune prostatitis.
abstract: Male rats were immunized with prostate tissue homogenate supernate (PTHS) of male rats with complete Freund's adjuvant (CFA) intra dermal in the multiple points and simultaneously immunized with 0.5 ml Pertussis-Diphtheria-Tetanus (PDT) vaccine intra peritonea on 0 and 30th day. At the 45th day after first immunization, animals were sacrificed and a series of examinations such as HE stain, assay of TNF-alpha by ELISA and assay of inducible nitric oxide synthase (iNOS) mRNA by in-situ hybridization (ISH) were taken. We observed that there was a remarkable up-regulation of TNF-alpha expression in the high dosage model group. The results of macropathology, histopathology and iNOS ISH also revealed the same tendency. This experimental procedure is effective to induce chronic abacterial prostatitis (CAP).
authors: College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang province, PRC.
authors: Xiao-hui
authors: XH
authors: Zhou
authors: Lian-da
authors: LD
authors: Li
authors: Li-mao
authors: LM
authors: Wu
authors: Lei
authors: L
authors: Han
authors: Zhong-de
authors: ZD
authors: Liu
authors: Ji-xiang
authors: JX
authors: Yang
authors: Yan-wei
authors: YW
authors: Lv
authors: Chun-lai
authors: CL
authors: You
authors: Zhi-heng
authors: ZH
authors: Zhou
mesh_terms: D000818:Animals; D001327:Autoimmune Diseases / Q000473:pathology / Q000503:physiopathology*; D004195:Disease Models, Animal; D017403:In Situ Hybridization; D007249:Inflammation / Q000503:physiopathology*; D008297:Male; D052247:Nitric Oxide Synthase Type II / Q000235:genetics; D011472:Prostatitis / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D014409:Tumor Necrosis Factor-alpha; D052247:Nitric Oxide Synthase Type II