Mapping of a quantitative trait locus for beef marbling on bovine chromosome 9 in purebred Japanese black cattle.
The goal of this study is to detect quantitative trait loci (QTL) for carcass traits applicable for a DNA-based breeding system in a Japanese Black cattle population. A purebred paternal half-sib family from a commercial line composed of 65 steers was initially analyzed using 188 informative microsatellites giving a 16-cM average interval covering 29 autosomes. A significant QTL for marbling was detected in the centromeric portion of bovine chromosome (BTA) 9. After additional marker genotyping across a larger sample size composed of 169 individuals, this locus was refined to a 20-cM confidence interval between microsatellites BM1227 (24 cM) and DIK2741 (50 cM) at a 1% chromosome-wise threshold. The allele substitution effect between Q and q for a beef marbling standard score (1 to 12 range) on BTA9 was 1.0 (5.7% of total phenotypic variance in QTL contribution in this family). This result provides a primary platform for a marker-assisted selection system of the beef marbling trait within the Japanese Black (Wagyu) cattle population.
Imai
K
K
Hiroshima Prefectural Livestock Technology Research Center, Shobara, Hiroshima, Japan.
Matsughige
T
T
Watanabe
T
T
Sugimoto
Y
Y
Ihara
N
N
D000818:Animals; D001947:Breeding; D002417:Cattle / Q000235:genetics*; D033481:Chromosomes, Mammalian; D004247:DNA / Q000737:chemistry / Q000235:genetics; D008040:Genetic Linkage; D005838:Genotype; D008297:Male; D008460:Meat / Q000592:standards*; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction / Q000662:veterinary; D040641:Quantitative Trait Loci; D012044:Regression Analysis
D004247:DNA