title: Antibody responses in human cystic hydatid disease to the laminated layer of Echinococcus granulosus.
abstract: The laminated layer of hydatid cysts of Echinococcus granulosus represents a considerable amount of parasite material. Its antigenic role, however, is unclear. Extracts of laminated layer taken from sheep cysts were analysed in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS/PAGE) and were found to contain bands at 55 and 25-29 kDa, which reacted with an anti-sheep IgG antibody probe, indicating that these were likely to be host-contaminating components within the layer. However, the same bands were also recognised by a significant proportion of human hydatid patients, particularly by IgG4 antibodies, and not by negative control individuals. These individuals did not recognise immunoglobulin heavy and light chains in a sheep serum extract in the same manner. It seems likely that there are either host or parasite antigenic components at similar molecular weights or that certain parasite antigens may share epitopes with sheep immunoglobulins. The antigens at 25-29 kDa were found to be glycoproteins by lectin blot analysis and may be important markers of disease status.
authors: Medical Parasitology Department, Golestan University of Medical Sciences, Gorgan, Iran.
authors: H
authors: H
authors: Taherkhani
authors: E
authors: E
authors: Zeyhle
authors: M T
authors: MT
authors: Rogan
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000737:chemistry / Q000276:immunology*; D004443:Echinococcosis / Q000469:parasitology; D048209:Echinococcus granulosus / Q000737:chemistry / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000469:parasitology
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D006023:Glycoproteins