Analysis of calcium changes in endoplasmic reticulum during apoptosis by the fluorescent indicator chlortetracycline.
Many studies suggest that endoplasmic reticulum (ER) Ca2+ pool rather than cytosolic Ca2+ may play a crucial role in triggering apoptosis. In this study, we performed an image analysis of cells loaded with the fluorescent dye chlortetracycline (CTC) to in situ analyze Ca2+ changes within the ER in apoptosing promonocytic U937 cells. The results, validated through the use of thapsigargin (THG) as ER Ca2+ depletor, confirm the findings that apoptotic cells have a Ca2+-depleted ER, in contrast with treated but still viable cells.
Cerella
Claudia
C
Dipartimento di Biologia, Universita' di Roma Tor Vergata, via Ricerca Scientifica, 1, 00133 Roma (I), Italy. cerella@uniroma2.it
Mearelli
Cristina
C
De Nicola
Milena
M
D'Alessio
Maria
M
Magrini
Andrea
A
Bergamaschi
Antonio
A
Ghibelli
Lina
L
D017209:Apoptosis; D002118:Calcium / Q000378:metabolism*; D002751:Chlortetracycline / Q000737:chemistry*; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D020298:U937 Cells
D005456:Fluorescent Dyes; D002118:Calcium; D002751:Chlortetracycline