title: The protective role of heme oxygenase-1 induction on testicular tissues after testicular torsion and detorsion.
abstract: OBJECTIVE
  Testicular torsion-detorsion has been identified as an ischemia-reperfusion type of injury. We elucidated the protective role of heme oxygenase-1 super induction on testicular torsion-detorsion injury.
  
  
  METHODS
  Adult male Sprague-Dawley rats were randomly allocated to undergo testicular torsion-detorsion, immediately followed by injection of normal saline, the heme oxygenase-1 inducer hemin or hemin plus the heme oxygenase-1 inhibitor tin protoporphyrin. Another set of rats that underwent sham operation, immediately followed by injection of normal saline, hemin or hemin plus tin protoporphyrin, served as controls. Testes were harvested 4 and 24 hours after detorsion, respectively, in the experimental groups or at comparable time points in the control groups.
  
  
  RESULTS
  Histological evaluation confirmed that torsion-detorsion caused significant testicular tissue injury. Torsion-detorsion also caused significant increases in the testicular levels of nitric oxide, malondialdehyde, myeloperoxidase activity and heme oxygenase-1. The heme oxygenase-1 inducer hemin significantly enhanced the heme oxygenase-1 expression induced by torsion-detorsion and in turn attenuated testicular injury, and increases in nitric oxide, malondialdehyde and myeloperoxidase activity. In addition, the protective effects of hemin were significantly offset by the heme oxygenase-1 inhibitor tin protoporphyrin.
  
  
  CONCLUSIONS
  Super induction of heme oxygenase-1 protects testes from torsion-detorsion injury.
authors: Department of Urology, Mackay Memorial Hospital, Mackay Medicine, Management and Nursing College, Taipei, Taiwan, Republic of China.
authors: Stone
authors: S
authors: Yang
authors: Hung-Jen
authors: HJ
authors: Shih
authors: Yung-Chiong
authors: YC
authors: Chow
authors: Pei-Shan
authors: PS
authors: Tsai
authors: Tao-Yeuan
authors: TY
authors: Wang
authors: Paulus S
authors: PS
authors: Wang
authors: Chun-Jen
authors: CJ
authors: Huang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors / Q000008:administration & dosage; D051547:Heme Oxygenase-1 / Q000187:drug effects / Q000378:metabolism*; D006427:Hemin / Q000008:administration & dosage; D015151:Immunoblotting; D007274:Injections, Intraperitoneal; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008665:Metalloporphyrins / Q000008:administration & dosage; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D011524:Protoporphyrins / Q000008:administration & dosage; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D013053:Spectrophotometry; D013085:Spermatic Cord / Q000378:metabolism* / Q000473:pathology; D013086:Spermatic Cord Torsion / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D016896:Treatment Outcome
chemical_list: D015415:Biomarkers; D004791:Enzyme Inhibitors; D008665:Metalloporphyrins; D011524:Protoporphyrins; D009569:Nitric Oxide; D008315:Malondialdehyde; D006427:Hemin; C032628:tin protoporphyrin IX; D009195:Peroxidase; D051547:Heme Oxygenase-1