[Determination of matrine, sophoridine and oxymatrine in Compound Kushen Injection by HPLC].
OBJECTIVE
To establish a high performance liquid chromatography (HPLC) method for determination of matrine, sophoridine and oxymatrine in Compound Kushen Injection.


METHODS
Alltima amino column was used with acetonitrile-3% phosphoric acid-ethyl alcohol (80:10:10) as the mobile phase and the detection wavelength was at 220 nm.


RESULTS
The mean recovery of matrine, sophoridine and oxymatrine in Compound Kushen Injection was 99.51% (RSD 1. 58%), 99.24% (RSD 1.44%) and 100.22% (RSD 1.85%), respectively.


CONCLUSIONS
The method was simple, rapid, accurate and specific and suitable for quality control of the product.
Tian
Juan
J
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Wang
Wei-hao
WH
Gao
Hui-min
HM
Wang
Zhi-min
ZM
D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D011786:Quality Control; D011807:Quinolizines / Q000032:analysis*; D015203:Reproducibility of Results; D000093842:Matrines
D000470:Alkaloids; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D011807:Quinolizines; C037573:oxymatrine; D000093842:Matrines