title: Rapid measurement of total antioxidant capacity in plants.
abstract: There is growing interest in measuring the antioxidant status of plant tissues. This protocol describes the oxygen radical absorbance capacity (ORAC) assay, which measures antioxidant inhibition of peroxyl radical-induced oxidations and is a measure of total antioxidant capacity. The assay is performed in a microplate and is assessed with a 96-well multi-detection plate reader. Total antioxidant capacity of 64 experimental samples can easily be analyzed in 1 d. This assay is presented along with rapid assays for total phenolic content and total ascorbate content. Overall, these assays provide a general diagnostic tool of the antioxidant capacity in leaf tissue extracts.
authors: Program in Physiological and Molecular Plant Biology and Institute for Genomic Biology, University of Illinois Urbana-Champaign, 147 ERML, 1201 W. Gregory Drive, Urbana, Illinois 61801, USA.
authors: Kelly M
authors: KM
authors: Gillespie
authors: June M
authors: JM
authors: Chae
authors: Elizabeth A
authors: EA
authors: Ainsworth
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D005456:Fluorescent Dyes / Q000032:analysis; D010944:Plants / Q000737:chemistry / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D000975:Antioxidants; D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species