Analysis of cadmium and lead in mice organs: effect of Nigella sativa L. (Black Cumin) on the distribution and immunosuppressive effect of cadmium-lead mixture in mice.
Analysis and distribution of Pb and Cd in different mice organs, including the liver, kidney, spleen, heart, and blood, were evaluated before and after treatment with different aqueous concentrations of Nigella sativa (1.25-10.0 mg/L). Atomic absorption spectrometry was used for analysis of Pb and Cd in these organs. Results indicated that the Pb in the unexposed group of mice without treatment with N. sativa (black cumin) was in the following order: liver > heart > spleen > kidney, and the distribution of Pb in various organs of the unexposed group was not affected significantly by N. sativa. Moreover, results of mice exposed for Pb show that the Pb concentrations in different organs were reduced significantly (p < 0.05) by 72.9%, 63.4%, 72.3%, 66.7%, and 39.5% at a dose of 10 mg/L of N. sativa for the liver, kidney, heart, spleen, and blood, respectively. Furthermore, the distribution of Cd in the unexposed Cd group of mice without treatment with N. sativa was in the following order: kidney > heart > spleen > liver. Nigella sativa at 10 mg/L reduced Cd levels in mice exposed to Cd by 75.5%, 83.3%, 47.0%, 95.3%, and 100% in the liver, kidney, heart, spleen, and blood, respectively, whereas blood Cd concentrations were lowered to below the detection limit of 0.05 mug/L. A 28-d exposure of mice to a Cd-Pb mixture at a concentration of 1 ppm in drinking water induced a highly significant inhibition (p < 0.0001) of antibody response to human serum (80.5%). The suppressed immune responses in mice pretreated with the Cd-Pb mixture were reversed by 43.1% and 38.9% in the presence of 1.25 and 2.5 mg/mL of N. sativa, respectively, whereas higher concentrations (5-10 mg/mL) of N. sativa increased the immunosuppression significantly. Nigella sativa at 1.25-10 mg/mL did not induce any significant modulation of the antibody response in unexposed mice.
Massadeh
Adnan M
AM
Department of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan.
Al-Safi
Saafan A
SA
Momani
Idrees F
IF
Al-Mahmoud
Mohsen
M
Alkofahi
Ahmad S
AS
D000818:Animals; D002104:Cadmium / Q000493:pharmacokinetics*; D004305:Dose-Response Relationship, Drug; D005260:Female; D007107:Immune System / Q000187:drug effects; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007854:Lead / Q000493:pharmacokinetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D031881:Nigella sativa; D009928:Organ Specificity / Q000187:drug effects
D007166:Immunosuppressive Agents; D002104:Cadmium; D007854:Lead