title: Up-regulation of PTEN at the transcriptional level is an adverse prognostic factor in female lung adenocarcinomas.
abstract: In lung adenocarcinomas, genetic alterations of PTEN are relatively rare and little has been reported concerning the relationship between PTEN transcriptional level and clinicopathologic features or genetic changes. This study was conducted to gain insight into clinicopathologic correlations. The transcriptional levels of PTEN were examined using real time RT-PCR and analyzed for correlations with clinicopathologic features and the mutation status of EGFR and KRAS. After confirming significant correlation for PTEN levels between macrodissected and microdissected materials (p<0.01), macrodissected samples from 115 lung adenocarcinomas were examined. There were no significant difference between the PTEN levels, divided into three ranges, and the mutation status of EGFR or KRAS. Noteworthy clinicopathologic correlations between PTEN transcriptional up/down-regulation and young age (p=0.0081, 61.7+/-8.7years versus 66.1+/-8.1years), smoking (p=0.032) and less differentiated adenocarcinomas (p=0.013) were identified. Whereas male patients demonstrated no prognostic association with PTEN levels, female cases with up-regulated PTEN expression had significantly worse survival compared with those with normal PTEN levels (p=0.0027). This study revealed distinct clinicopathologic correlations with PTEN transcriptional up/down-regulation.
authors: Department of Pathology, The Cancer Institute, Japanese Foundation for Cancer Research (JFCR), 3-10-6 Ariake, Koto-ku, Tokyo 135-8550, Japan.
authors: Kentaro
authors: K
authors: Inamura
authors: Yuki
authors: Y
authors: Togashi
authors: Kimie
authors: K
authors: Nomura
authors: Hironori
authors: H
authors: Ninomiya
authors: Miyako
authors: M
authors: Hiramatsu
authors: Michiyo
authors: M
authors: Okui
authors: Yukitoshi
authors: Y
authors: Satoh
authors: Sakae
authors: S
authors: Okumura
authors: Ken
authors: K
authors: Nakagawa
authors: Eiju
authors: E
authors: Tsuchiya
authors: Yuichi
authors: Y
authors: Ishikawa
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D051059:PTEN Phosphohydrolase / Q000235:genetics*; D011379:Prognosis; D016019:Survival Analysis; D014158:Transcription, Genetic; D015854:Up-Regulation
chemical_list: D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human