Salt and drought tolerance of transgenic salvia miltiorrhiza Bunge with the TaLEA1 gene.
TaLEA1, a gene encoding a late-embryogenesis-abundant protein, was cloned from wheat and was transformed into Salvia Miltiorrhiza Bunge by Agrobacterium-mediated leaf-disk transformation method. Seven transgenic lines were obtained after kanamycin (50 mg/L) screening. Six positive lines were obtained by PCR amplification, and after four additional generations, one stable line was obtained by Southern hybridization. Transgenic plants had better growth states than control plants on mediums containing 1% NaCl and 8% PEG6000, which demonstrated that TaLEA1 played an important role in increasing the salt and drought tolerance of S. miltiorrhiza.
Han
Li-Min
LM
College of Life Sciences, Shaanxi Normal University, Xi'an 710062, China.
Yu
Jia-Ning
JN
Ju
Wen-Feng
WF
D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D055864:Droughts; D010940:Plant Proteins / Q000235:genetics / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D016133:Polymerase Chain Reaction; D012231:Rhizobium / Q000235:genetics; D027561:Salvia miltiorrhiza / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D012965:Sodium Chloride / Q000494:pharmacology*; D014170:Transformation, Genetic
D010940:Plant Proteins; D012965:Sodium Chloride