title: Correlation of tracheal smooth muscle function with structure and protein expression during early development.
abstract: With increased survival of premature infants, understanding the impact of development on airway function and structure is imperative. Airway smooth muscle plays a primary role in the modulation of airway function. The purpose of this study is to correlate the functional maturation of airway smooth muscle during the perinatal period with structural alterations at the cellular, ultrastructural, and molecular levels. Length-tension and dose-response analyses were performed on tracheal rings acquired from preterm and term newborn lambs. Subsequent structural analyses included isolated airway smooth muscle cell length, electron microscopy, and myosin heavy chain isoform expression measurements. Functionally the compliance, contractility, and agonist sensitivity of the tracheal rings matured during preterm to term development. Structurally, isolated cell lengths and electron microscopic ultrastructure were not significantly altered during perinatal development. However, expression of myosin heavy chain isoforms increased significantly across the age range analyzed, correlating with the maturational increase in smooth muscle contractility. In conclusion, the developmental alterations in tracheal function appear due, in part, to enhanced smooth muscle myosin heavy chain expression.
authors: Department of Physiology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA.
authors: Aaron B
authors: AB
authors: Cullen
authors: Peter H
authors: PH
authors: Cooke
authors: Steven P
authors: SP
authors: Driska
authors: Marla R
authors: MR
authors: Wolfson
authors: Thomas H
authors: TH
authors: Shaffer
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D008170:Lung Compliance / Q000502:physiology; D023421:Models, Animal; D009119:Muscle Contraction / Q000502:physiology; D009130:Muscle, Smooth / Q000502:physiology* / Q000648:ultrastructure; D018995:Myosin Heavy Chains / Q000378:metabolism; D012756:Sheep; D014132:Trachea / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D018995:Myosin Heavy Chains