Diagnosis of canine brucellosis by ELISA using an antigen obtained from wild Brucella canis.
An indirect ELISA test was developed for the diagnosis of Brucella canis infection in dogs. A bacterial whole cell extract was used as a solid phase antigen, using B. canis isolated from an infected animal. Sera from culture-positive and healthy negative animals were used as internal reference controls. The cut-off point was determined by a mathematical formula for a statistically valid value, which defined the upper prediction limit, based on the upper tail of the t-distribution of 21 negative control sera readings, for the confidence level of 99.5%. The sensitivity and specificity of the ELISA test were 95% and 91%, respectively. The ELISA test showed a significant concordance index (K=0.84) with the agar gel immunodiffusion test. The reliability of the ELISA for the detection of infected animals was established by a double blind study testing 280 sera provided by serum banks from different diagnostic and research institutions and analyzed by ROC Curve.
Barrouin-Melo
Stella Maria
SM
Departamento de Patologia e Clínicas, Escola de Medicina Veterinária, Universidade Federal da Bahia, Av. Adhemar de Barros, 500 Salvador, BA, CEP 40170-110, Brazil. barrouin@ufba.br
Poester
Fernando Padilla
FP
Ribeiro
Marcos Borges
MB
de Alcântara
Adriano Costa
AC
Aguiar
Paulo Henrique Palis
PH
Nascimento
Ivana Lúcia
IL
Schaer
Robert Eduard
RE
Nascimento
Roberto Meyer
RM
Freire
Songelí Menezes
SM
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D041002:Brucella canis / Q000276:immunology*; D002006:Brucellosis / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D004283:Dog Diseases / Q000097:blood / Q000175:diagnosis*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D012680:Sensitivity and Specificity
D000942:Antigens, Bacterial