title: Rapid assay format for multiplex detection of humoral immune responses to infectious disease pathogens (HIV, HCV, and TB).
abstract: A novel assay is described for multiplex detection of antibodies against different pathogens from a single sample. The assay employs a modified lateral flow format (consecutive flow, CF) together with a sensitive reporter particle technology (up-converting phosphor technology, UPT) that allows for fully instrumented assay analysis. Lateral flow (LF) strips developed for the detection of human antibodies against human immunodeficiency virus type-1 and -2 (HIV-1 and -2) with additional capture zones to detect antibodies against Myobacterium tuberculosis (TB) and hepatitis C Virus (HCV) provided the strips to test multiplexing. Data are presented that show the performance of the TB and HCV test, as well as two multiplex assays, TB with HIV and HCV with HIV. The TB/HCV assays demonstrate excellent detection capability, and HIV multiplexing does not affect the qualitative test result. The bench-top CF format was converted to a microfluidic platform and a first prototype semiautomated chip capable of performing CF is presented here.
authors: Department of Molecular Cell Biology, Leiden University Medical Center, RC Leiden, the Netherlands. Corstjens@LUMC.NL
authors: Paul L A M
authors: PL
authors: Corstjens
authors: Zongyuang
authors: Z
authors: Chen
authors: Michel
authors: M
authors: Zuiderwijk
authors: Haim H
authors: HH
authors: Bau
authors: William R
authors: WR
authors: Abrams
authors: Daniel
authors: D
authors: Malamud
authors: R
authors: R
authors: Sam Niedbala
authors: Hans J
authors: HJ
authors: Tanke
mesh_terms: D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000097:blood*; D006678:HIV / Q000276:immunology*; D015483:HIV Antibodies / Q000096:biosynthesis / Q000097:blood*; D016174:Hepacivirus / Q000276:immunology*; D018937:Hepatitis C Antibodies / Q000096:biosynthesis / Q000097:blood*; D006801:Humans; D044085:Microfluidics / Q000295:instrumentation; D009169:Mycobacterium tuberculosis / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D015483:HIV Antibodies; D018937:Hepatitis C Antibodies