title: Skin prick testing predicts peanut challenge outcome in previously allergic or sensitized children with low serum peanut-specific IgE antibody concentration.
abstract: Peanut allergy is transient in some children but it is not clear whether quantitating peanut-specific IgE by Skin Prick Test (SPT) adds additional information to fluorescent-enzyme immunoassay (FEIA) in discriminating between allergic and tolerant children. To investigate whether SPT with a commercial extract or fresh foods adds additional predictive information for peanut challenge in children with a low FEIA (<10 k UA/L) who were previously sensitized, or allergic to peanuts. Children from a hospital-based allergy service who were previously sensitized or allergic to peanuts were invited to undergo a peanut challenge unless they had a serum peanut-specific IgE>10 k UA/L, a previous severe reaction, or a recent reaction to peanuts (within two years). SPT with a commercial extract, raw and roasted saline soaked peanuts was performed immediately prior to open challenge in hospital with increasing quantity of peanuts until total of 26.7 g of peanut was consumed. A positive challenge consisted of an objective IgE mediated reaction occurring during the observation period. 54 children (median age of 6.3 years) were admitted for a challenge. Nineteen challenges were positive, 27 negative, five were indeterminate and three did not proceed after SPT. Commercial and fresh food extracts provided similar diagnostic information. A wheal diameter of >or=7 mm of the commercial extract predicted an allergic outcome with specificity 97%, positive predictive value 93% and sensitivity 83%. There was a tendency for an increase in SPT wheal since initial diagnosis in children who remained allergic to peanuts while it decreased in those with a negative challenge. The outcome of a peanut challenge in peanut sensitized or previously allergic children with a low FEIA can be predicted by SPT. In this cohort, not challenging children with a SPT wheal of >or=7 mm would have avoided 15 of 18 positive challenges and denied a challenge to one out of 27 tolerant children.
authors: Department of Immunology, Princess Margaret Hospital for Children, Perth, WA, Australia.
authors: Richard C
authors: RC
authors: Nolan
authors: Peter
authors: P
authors: Richmond
authors: Susan L
authors: SL
authors: Prescott
authors: Dominic F
authors: DF
authors: Mallon
authors: Grace
authors: G
authors: Gong
authors: Annkathrin M
authors: AM
authors: Franzmann
authors: Rama
authors: R
authors: Naidoo
authors: Richard K S
authors: RK
authors: Loh
mesh_terms: D001315:Australia; D002648:Child; D005260:Female; D006801:Humans; D007114:Immunization; D007073:Immunoglobulin E / Q000097:blood*; D008297:Male; D021183:Peanut Hypersensitivity / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology*; D011237:Predictive Value of Tests; D012882:Skin Tests
chemical_list: D007073:Immunoglobulin E