title: ATPase-coupled release control from polyion complex capsules encapsulating muscle proteins.
abstract: In the present study, a muscle contractile protein complex, actomyosin, has been successfully encapsulated into gellan-chitosan polyion complex (PIC) capsules. The recovery of the myosin-ATPase activity is approximately 50% and the Mg2+-ATPase activity is stimulated by the presence of F-actin, which implies the formation of the actomyosin complex inside the capsule. Furthermore, encapsulation could protect the myosin, F-actin, and actomyosin inside from hydrolysis by proteases. Two small proteins, myoglobin and cytochrome c, have been used in the release tests. The release of myoglobin is not affected by the ionic strength of the external solution, while the release of cytochrome c increases with increasing ionic strength. The maximal releases are found in the external pH solution close to the isoelectric points of each protein. The Mg2+-ATP complex itself reduces the release percentages of the small proteins from the PIC capsule. The release amounts further decrease when coexisting with Mg2+-ATP and the encapsulated actomyosin, which indicates the release regulation by actomyosin. The present study suggests that the ATPase-coupled sliding motion of the myosin-F-actin filaments modifies the pore size of the polymer networks in the PIC capsule membranes.
authors: Department of Kansei Engineering, Faculty of Textile Science and Technology, Shinshu University, Japan.
authors: Kousuke
authors: K
authors: Sugiura
authors: Kousaku
authors: K
authors: Ohkawa
authors: Toshihiro
authors: T
authors: Hirai
authors: Toshihiro
authors: T
authors: Fujii
mesh_terms: D000199:Actins / Q000737:chemistry*; D000255:Adenosine Triphosphate / Q000737:chemistry; D000818:Animals; D002214:Capsules; D048271:Chitosan / Q000737:chemistry*; D045304:Cytochromes c / Q000737:chemistry*; D004800:Enzymes, Immobilized; D006863:Hydrogen-Ion Concentration; D009218:Myosins / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D016062:Porosity; D011817:Rabbits
chemical_list: D000199:Actins; D002214:Capsules; D004800:Enzymes, Immobilized; D011135:Polysaccharides, Bacterial; C048288:gellan gum; D000255:Adenosine Triphosphate; D045304:Cytochromes c; D048271:Chitosan; D009218:Myosins