title: [Molecular and morphological comparison of hookworms from genus Uncinaria invading red fox (Vulpes vulpes) and dog (Canis familiaris)].
abstract: Two species of hookworms from genus Uncinaria have been found so far in Poland. Uncinaria stenocephala infects mainly dog, wolf and red fox, whereas Uncinaria criniformis is a parasite of mustelids (but it was also reported from red fox). 19 male and 29 female hookworms from red foxes have been compared with 10 male and 12 female worms from dogs. Hookworms from dogs were generally smaller than these from foxes, but no other morphological differences could be found. These hookworms were qualified to species Uncinaria stenocephala on the ground of morphology of male. Genomic DNA samples have been isolated from these hookworms and segments of rDNA including part of small subunit of ribosomal RNA gene; internal transcribed spacer 1 (ITS1); 5.8 S ribosomal RNA; internal transcribed spacer 2 (ITS2) and part of large subunit of ribosomal RNA have been amplified and sequenced. Sequences from Uncinaria obtained both from foxes and dogs have shown very high similarity to the sequence of Uncinaria stenocephala, so all examined hookworms have been classified as belonging to this species.
authors: Zakład Parazytologii i Inwazjologii, Katedra Nauk Przedklinicznych, Wydział Medycyny Weterynaryjnej, Szkoła Główna Gospodarstwa Wiejskiego, Ciszewskiego 8, 02-786 Warszawa.
authors: Paweł
authors: P
authors: Górski
authors: Agnieszka
authors: A
authors: Radowańska
authors: Dorota
authors: D
authors: Jaros
authors: Marcin
authors: M
authors: Wiśniewski
mesh_terms: D000723:Ancylostomatoidea / Q000033:anatomy & histology / Q000235:genetics* / Q000648:ultrastructure*; D000818:Animals; D018509:DNA, Helminth / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D004283:Dog Diseases / Q000469:parasitology*; D004285:Dogs / Q000469:parasitology*; D005260:Female; D005589:Foxes / Q000469:parasitology*; D005819:Genetic Markers; D006725:Hookworm Infections / Q000453:epidemiology / Q000469:parasitology* / Q000662:veterinary*; D006790:Host-Parasite Interactions; D008297:Male; D009929:Organ Size / Q000235:genetics; D011044:Poland / Q000453:epidemiology; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D018509:DNA, Helminth; D004275:DNA, Ribosomal; D005819:Genetic Markers