Association between oxidative stress in pregnancy and preterm premature rupture of membranes.
BACKGROUND
Premature rupture of membranes (PROM) is caused by collagen damage in the chorioamniotic sac leading to tearing. Reactive oxygen species (ROS) may be the cause of collagen damage. Isoprostanes (F(2)-IP) are produced by ROS attack on polyunsaturated fatty acids and are sensitive and specific biomarkers of lipid-peroxidation in vivo.


OBJECTIVE
To verify whether oxidative stress occurs in pregnancies associated with preterm PROM.


METHODS
F(2)-IPs were measured in amniotic fluid of 16 pregnancies with preterm PROM (Group II) and 97 without PROM (Group I).


RESULTS
F(2)-IP concentrations (pg/mL) were significantly higher in group II than group I (p<0.0001). The ROC curve showed a sensitivity of 100% and a specificity of 84.5% at a cut-off of 124.4 pg/mL.


CONCLUSIONS
An association exists between oxidative stress in pregnancy and preterm PROM. The detection of amniotic fluid F(2)-IP concentrations seems to be a reliable predictive index of risk of preterm PROM.
Longini
Mariangela
M
Department of Pediatrics, Obstetrics and Reproductive Medicine, University of Siena, Policlinico Le Scotte, V le Bracci 36, Siena, Italy.
Perrone
Serafina
S
Vezzosi
Piero
P
Marzocchi
Barbara
B
Kenanidis
Antonio
A
Centini
Giovanni
G
Rosignoli
Lucia
L
Buonocore
Giuseppe
G
D000293:Adolescent; D000328:Adult; D000653:Amniotic Fluid / Q000378:metabolism; D028441:F2-Isoprostanes / Q000378:metabolism; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007231:Infant, Newborn; D018384:Oxidative Stress; D011247:Pregnancy
D028441:F2-Isoprostanes