Sensitive determination of ultra-trace nitric oxide in blood using derivatization-polymer monolith microextraction coupled with reversed-phase high-performance liquid chromatography.
Nitric oxide (NO) plays a very important role in human blood system. In this work, a novel approach has been developed for the quantitation of ultra-trace NO derivatized with 1,3,5,7-tetramethyl-8-(3',4'-diaminophenyl)-difluoroboradiaza-s-indacene (DAMBO) using a polymer monolith microextraction (PMME) with a poly(methacrylic acid-ethylene glycol dimethacrylate) (MAA-EGDMA) monolith in conjunction with high-performance liquid chromatography (HPLC). Both derivatization and PMME conditions have been optimized in detail. The detection limit of derivatized NO was 2x10(-12) mol L(-1) (signal to noise=3) and linear range was 9x10(-11)-4.5x10(-8) mol L(-1). The proposed DAMBO-based derivatization-PMME-HPLC-fluorescence detection method has been successfully applied for the determination of NO in 10 microL blood samples of healthy persons and patients suffering from ischemic cardio-cerebrovascular diseases with recoveries varying from 87.40 to 91.60%.
Huang
Ke-Jing
KJ
Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
Zhang
Min
M
Xie
Wan-Zhen
WZ
Zhang
Hua-Shan
HS
Feng
Yu-Qi
YQ
Wang
Hong
H
D002851:Chromatography, High Pressure Liquid; D003920:Diabetes Mellitus / Q000097:blood; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood / Q000150:complications; D006973:Hypertension / Q000097:blood / Q000150:complications; D008832:Microchemistry; D009569:Nitric Oxide / Q000097:blood*; D011109:Polymethacrylic Acids; D012680:Sensitivity and Specificity; D052617:Solid Phase Microextraction; D020521:Stroke / Q000097:blood
D011109:Polymethacrylic Acids; C497090:poly((ethylene glycol dimethacrylate)-co-(methacrylic acid)); D009569:Nitric Oxide