title: Comparison of acute structural and histopathological changes of the porcine ciliary processes after endoscopic cyclophotocoagulation and transscleral cyclophotocoagulation.
abstract: BACKGROUND
  The objective of this study is to investigate the acute histological effects of transscleral cyclophotocoagulation and endoscopic cyclophotocoagulation on the ciliary body and other structures of porcine eyes compared with untreated controls.
  
  
  METHODS
  Transscleral cyclophotocoagulation and endoscopic cyclophotocoagulation were performed on porcine eyes. Detailed histological evaluations were performed with light and scanning electron microscopy of treated eyes and compared with untreated controls.
  
  
  RESULTS
  Histological changes were observed with both light and scanning electron microscopy for all treated tissues. Tissue treated with transscleral cyclophotocoagulation showed pronounced tissue disruption of the ciliary body muscle and stroma, ciliary processes, and both pigmented and non-pigmented ciliary epithelium. Endoscopic cyclophotocoagulation-treated tissue exhibited pronounced contraction of the cilliary processes with disruption of the ciliary body epithelium, with less architectural disorginization and sparing of the ciliary body muscle. The sclera was not affected by either laser treatment.
  
  
  CONCLUSIONS
  The endoscopic cyclophotocoagulation treatment caused less damage to the ciliary body compared with the transscleral cyclophotocoagulation when evaluated by light and scanning electron microscopy. Compared with transscleral cyclophotocoagulation, endoscopic cyclophotocoagulation appears to be a more selective form of cyclophotocoagulation resulting in less tissue disruption while achieving the goal of destroying ciliary body epithelium.
authors: UPMC Eye Center, Eye and Ear Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA. minapantcheva@yahoo.com
authors: Mina B
authors: MB
authors: Pantcheva
authors: Malik Y
authors: MY
authors: Kahook
authors: Joel S
authors: JS
authors: Schuman
authors: Marc W
authors: MW
authors: Rubin
authors: Robert J
authors: RJ
authors: Noecker
mesh_terms: D000818:Animals; D002924:Ciliary Body / Q000601:surgery* / Q000648:ultrastructure*; D004724:Endoscopy / Q000379:methods*; D017075:Laser Coagulation / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D013508:Ophthalmologic Surgical Procedures; D012590:Sclera; D013552:Swine