Characterization of mature rat oligodendrocytes: a proteomic approach.
Oligodendrocytes are glial cells responsible for the synthesis and maintenance of myelin in the central nervous system (CNS). Oligodendrocytes are vulnerable to damage occurring in a variety of neurological diseases. Understanding oligodendrocyte biology is crucial for the dissemination of de- and remyelination mechanisms. The goal of the present study is the construction of a protein database of mature rat oligodendrocytes. Post-mitotic oligodendrocytes were isolated from mature Wistar rats and subjected to immunocytochemistry. Proteins were extracted and analyzed by means of two-dimensional gel electrophoresis and two-dimensional liquid chromatography, both coupled to mass spectrometry. The combination of the gel-based and gel-free approach resulted in confident identification of a total of 200 proteins. A minority of proteins were identified in both proteomic strategies. The identified proteins represent a variety of functional groups, including novel oligodendrocyte proteins. The results of this study emphasize the power of the applied proteomic strategy to study known or to reveal new proteins and to investigate their regulation in oligodendrocytes in different disease models.
Dumont
Debora
D
Hasselt University, Biomedical Research Institute BIOMED, Transnationale Universiteit Limburg, School of Life Sciences, Diepenbeek, Belgium.
Noben
Jean-Paul
JP
Moreels
Marjan
M
Vanderlocht
Joris
J
Hellings
Niels
N
Vandenabeele
Frank
F
Lambrichts
Ivo
I
Stinissen
Piet
P
Robben
Johan
J
D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D002853:Chromatography, Liquid / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D007150:Immunohistochemistry / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000145:classification / Q000378:metabolism*; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar
D009419:Nerve Tissue Proteins