Development of a Daphnia magna DNA microarray for evaluating the toxicity of environmental chemicals.
Toxic chemical contaminants have a variety of detrimental effects on various species, and the impact of pollutants on ecosystems has become an urgent issue. However, the majority of studies regarding the effects of chemical contaminants have focused on vertebrates. Among aquatic organisms, Daphnia magna has been used extensively to evaluate organism- and population-level responses of invertebrates to pollutants in acute toxicity or reproductive toxicity tests. Although these types of tests can provide information concerning hazardous concentrations of chemicals, they provide no information about their mode of action. Recent advances in molecular genetic techniques have provided tools to better understand the responses of aquatic organisms to pollutants. In the present study, we adapted some of the techniques of molecular genetics to develop new tools, which form the basis for an ecotoxicogenomic assessment of D. magna. Based on a Daphnia expressed sequence tag database, we developed an oligonucleotide-based DNA microarray with high reproducibility. The DNA microarray was used to evaluate gene expression profiles of neonatal daphnids exposed to several different chemicals: Copper sulfate, hydrogen peroxide, pentachlorophenol, or beta-naphthoflavone. Exposure to these chemicals resulted in characteristic patterns of gene expression that were chemical-specific, indicating that the Daphnia DNA microarray can be used for classification of toxic chemicals and for development of a mechanistic understanding of chemical toxicity on a common freshwater organism.
Watanabe
Hajime
H
Okazaki Institute of Integrative Bioscience, National Institutes of Natural Sciences, Higashiyama 5-1, Myodaiji, Okazaki 444-8585, Japan. watanabe@nibb.ac.jp
Takahashi
Eri
E
Nakamura
Yuko
Y
Oda
Shigeto
S
Tatarazako
Norihisa
N
Iguchi
Taisen
T
D000818:Animals; D016000:Cluster Analysis; D019327:Copper Sulfate / Q000633:toxicity; D003621:Daphnia / Q000187:drug effects* / Q000235:genetics*; D030541:Databases, Genetic; D004785:Environmental Pollutants / Q000633:toxicity*; D020224:Expressed Sequence Tags; D005618:Fresh Water; D020869:Gene Expression Profiling / Q000379:methods*; D006861:Hydrogen Peroxide / Q000633:toxicity; D007564:Japan; D009152:Mutagenicity Tests / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010416:Pentachlorophenol / Q000633:toxicity; D019324:beta-Naphthoflavone / Q000633:toxicity
D004785:Environmental Pollutants; D019324:beta-Naphthoflavone; D006861:Hydrogen Peroxide; D010416:Pentachlorophenol; D019327:Copper Sulfate