A novel double-stable T-REx/gb cell line expressing glycoprotein B of herpes simplex virus 1.
We established and characterized a new stable T-REx/gB cell line expressing gB of HSV-1 under tetracycline (Tet) control. The expression of complete gB (120 K) in T-REx/gB cells was detected by Western blot analysis with anti-HSV-1/ANG/gB monoclonal antibody as early as 2 days after Tet induction. Inducibility and tightness of Tet-regulated gB expression in T-REx/gB cell line was shown to be preserved after long-term culture (2 months) and after numerous freezing/thawing cycles as well. In this study, we described the conditions required for the generation of the T-REx/gB cell line, which can be useful as a host for the isolation and propagation of HSV-1 recombinant viruses defective in gB gene.
Tangelmayer
R
R
Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Durmanová
V
V
Knopf
C W
CW
Kúdelová
M
M
Rezuchová
I
I
D015153:Blotting, Western; D002460:Cell Line / Q000821:virology*; D005786:Gene Expression Regulation; D018259:Herpesvirus 1, Human / Q000235:genetics; D006801:Humans; D008856:Microscopy, Fluorescence; D013752:Tetracycline / Q000494:pharmacology; D014759:Viral Envelope Proteins / Q000096:biosynthesis* / Q000235:genetics
D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus; D013752:Tetracycline