Identification of protein components in in vivo human acquired enamel pellicle using LC-ESI-MS/MS.
The acquired enamel pellicle is a thin protein film forming upon exposure of tooth enamel surfaces to saliva. The structural analysis of this integument relies on efficient pellicle harvesting and protein identification procedures. Material from three individual subjects and two pooled samples yielded the identification by LC-ESI-MS/MS of 130 pellicle proteins of which 89 were found in three or more experiments. A high intersubject consistency in pellicle composition was observed.
Siqueira
Walter L
WL
Department of Periodontology and Oral Biology, Goldman School of Dental Medicine, Boston University, Boston, Massachusetts 02118, USA.
Zhang
Weimin
W
Helmerhorst
Eva J
EJ
Gygi
Steven P
SP
Oppenheim
Frank G
FG
D000328:Adult; D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D044622:Dental Pellicle / Q000737:chemistry*; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D036103:Nanotechnology / Q000379:methods; D010455:Peptides / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D010455:Peptides; D011506:Proteins