In vitro antibacterial efficacy of MONOCRYL plus antibacterial suture (Poliglecaprone 25 with triclosan).
BACKGROUND
This study evaluated the in vitro efficacy of poliglecaprone 25 suture with triclosan against gram-positive and gram-negative bacteria.


METHODS
Poliglecaprone 25 sutures with and without triclosan were tested for in vitro efficacy against Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus epidermidis, methicillin-resistant Staphylococcus epidermidis (MRSE), Klebsiella pneumoniae, and Escherichia coli by a zone of inhibition assay. The suture also was tested against Escherichia coli in a colonization assay in a dynamic model simulating in vivo conditions. An in vitro triclosan diffusion assay and a sustained efficacy assay were performed by concurrent high-performance liquid chromatography and zone of inhibition assay. To assess stability, antibacterial efficacy testing was performed on samples held more than five months at elevated temperature.


RESULTS
Poliglecaprone 25 suture with triclosan demonstrated significant in vitro efficacy against a range of bacteria. The suture sustained in vitro efficacy for 11 days, corresponding to the in vitro triclosan diffusion profile.


CONCLUSIONS
Triclosan reduced in vitro colonization of poliglecaprone 25 suture by several strains of bacteria compared with untreated control sutures.
Ming
Xintian
X
Corporate Microbiology and Sterilization Sciences, Ethicon Inc, Somerville, NJ 08876, USA. xming@ethus.jnj.com
Rothenburger
Stephen
S
Yang
Dachuan
D
D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D020099:Coated Materials, Biocompatible; D015169:Colony Count, Microbial; D004146:Dioxanes; D004755:Enterobacteriaceae / Q000187:drug effects; D006095:Gram-Positive Cocci / Q000187:drug effects; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests / Q000379:methods; D011091:Polyesters; D013530:Surgical Wound Infection / Q000517:prevention & control; D013537:Sutures / Q000382:microbiology*; D014260:Triclosan / Q000494:pharmacology*
D000891:Anti-Infective Agents, Local; D020099:Coated Materials, Biocompatible; D004146:Dioxanes; D011091:Polyesters; C095495:glycolide E-caprolactone copolymer; D014260:Triclosan