title: Phagocytosis of Fonsecaea pedrosoi conidia, but not sclerotic cells caused by Langerhans cells, inhibits CD40 and B7-2 expression.
abstract: Fonsecaea pedrosoi is the major etiological agent of chromoblastomycosis, a chronic, suppurative, granulomatous mycosis usually confined to skin and subcutaneous tissues, presenting a worldwide distribution. The host defense mechanisms in chromoblastomycosis have not been extensively investigated. Langerhans cells (LC) are bone-marrow-derived, dendritic antigen-presenting cells of the epidermis, which constitutively express major histocompatibility complex (MHC) class II, and comprise 1-3% of total epidermal cells. LC are localized in suprabasal layers of the epidermis and in mucosa, where they play important roles in skin immune responses. The purpose of the present study was to evaluate the interaction of F. pedrosoi conidia or sclerotic cells with LC purified from BALB/c mice skin. We demonstrate here that LC phagocytose F. pedrosoi conidia but not sclerotic cells in the first 3 h of interaction, inhibiting hyphae formation during 12-hour coculture from both forms, internalized or not. Also, LC maturation, analyzed using CD40 and B7-2 expression, was inhibited by conidia, but not by sclerotic cells, indicating an important innate immunity function of LC against F. pedrosoi infection in these mice.
authors: Laboratório de Dermato-Imunologia Universidade do Estado do Pará (UEPA), Universidade Federal do Pará (UFPA) and Unidade de Referência em Dermatologia Sanitária do Estado do Pará Dr Marcello Candia (MC), Marituba, Pará, Brazil.
authors: Jorge Pereira
authors: JP
authors: da Silva
authors: Moises Batista
authors: MB
authors: da Silva
authors: Ubirajara Imbiriba
authors: UI
authors: Salgado
authors: José Antonio Picanço
authors: JA
authors: Diniz
authors: Sonia
authors: S
authors: Rozental
authors: Claudio Guedes
authors: CG
authors: Salgado
mesh_terms: D000818:Animals; D001203:Ascomycota / Q000276:immunology* / Q000648:ultrastructure; D051940:B7-2 Antigen / Q000096:biosynthesis* / Q000276:immunology; D019013:CD40 Antigens / Q000096:biosynthesis* / Q000276:immunology; D002862:Chromoblastomycosis / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D005260:Female; D006801:Humans; D025301:Hyphae / Q000648:ultrastructure; D007801:Langerhans Cells / Q000276:immunology* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D010587:Phagocytosis / Q000276:immunology; D013172:Spores, Fungal / Q000648:ultrastructure
chemical_list: D051940:B7-2 Antigen; D019013:CD40 Antigens