title: The BH3-only protein bid is dispensable for DNA damage- and replicative stress-induced apoptosis or cell-cycle arrest.
abstract: Bid, a caspase-activated proapoptotic BH3-only protein, is essential for Fas-induced hepatocyte destruction. Recent studies published in Cell produced conflicting results, indicating that loss of Bid either protects or enhances apoptosis induced by DNA damage or replicative stress. To resolve this controversy, we generated novel Bid-deficient mice on an inbred C57BL/6 background and removed the drug-selection cassette from the targeted locus. Nine distinct cell types from these Bid-deficient mice underwent cell-cycle arrest and apoptosis in a manner indistinguishable from control WT cells in response to DNA damage or replicative stress. Moreover, we found that even cells from the original Bid-deficient mice responded normally to these stimuli, indicating that differences in genetic background or the presence of a strong promoter within the targeted locus are unlikely to explain the differences between our results and those reported previously. We conclude that Bid has no role in DNA damage- or replicative stress-induced apoptosis or cell-cycle arrest.
authors: The Walter and Eliza Hall Institute of Medical Research, Parkville 3050, Australia.
authors: Thomas
authors: T
authors: Kaufmann
authors: Lin
authors: L
authors: Tai
authors: Paul G
authors: PG
authors: Ekert
authors: David C S
authors: DC
authors: Huang
authors: Fiona
authors: F
authors: Norris
authors: Ralph K
authors: RK
authors: Lindemann
authors: Ricky W
authors: RW
authors: Johnstone
authors: Vishva M
authors: VM
authors: Dixit
authors: Andreas
authors: A
authors: Strasser
mesh_terms: D000818:Animals; D017209:Apoptosis; D051023:BH3 Interacting Domain Death Agonist Protein / Q000235:genetics / Q000378:metabolism*; D001773:Blood Cells / Q000166:cytology; D002453:Cell Cycle; D049109:Cell Proliferation; D003600:Cytosol / Q000737:chemistry; D004249:DNA Damage; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008934:Mitogens / Q000378:metabolism
chemical_list: D051023:BH3 Interacting Domain Death Agonist Protein; D008934:Mitogens