Porphyromonas gingivalis infection and cell death in human aortic endothelial cells.
Porphyromonas gingivalis is a periodontal pathogen that promotes a proatherogenic response in endothelial cells. Cell death responses of human aortic endothelial cells to P. gingivalis at various multiplicities of infection (MOI) were investigated by assessment of cell detachment, histone-associated DNA fragmentation, lactate dehydrogenase release and ADP:ATP ratio. Porphyromonas gingivalis at MOI 1:10-1:100 did not have a cytotoxic effect, but induced apoptotic cell death at MOI 1:500 and 1:1000. Monocyte chemoattractant protein-1 production was significantly enhanced by P. gingivalis at MOI 1:100. At higher MOI, at least in vitro, P. gingivalis mediates endothelial apoptosis, thereby potentially amplifying proatherogenic mechanisms in the perturbed vasculature.
Roth
Georg A
GA
Division of Surgical Science, College of Physicians & Surgeons, Columbia University, New York, NY 10032, USA.
Ankersmit
Hendrik J
HJ
Brown
Vinette B
VB
Papapanou
Panos N
PN
Schmidt
Ann Marie
AM
Lalla
Evanthia
E
D000244:Adenosine Diphosphate / Q000032:analysis; D000255:Adenosine Triphosphate / Q000032:analysis; D001011:Aorta / Q000166:cytology; D017209:Apoptosis; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000096:biosynthesis; D053938:DNA Fragmentation; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D006657:Histones / Q000378:metabolism; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008858:Microscopy, Phase-Contrast; D016966:Porphyromonas gingivalis / Q000254:growth & development*
C111835:CCL2 protein, human; D018932:Chemokine CCL2; D006657:Histones; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase