Phagocyte-derived oxidants and plasma antioxidants in haemodialysed patients.
OBJECTIVE
Oxidative stress is one of the important complications occurring in haemodialysis. The aim of the study was to determine haemodialysis-induced changes in oxidative burst of phagocytes and the antioxidative properties of plasma.


METHODS
Twenty-seven patients and 50 healthy controls were examined. Oxidative burst of phagocytes and plasma antioxidative potential were measured luminometrically. Concentrations of major plasma antioxidants (vitamin E, bilirubin and uric acid) were also determined.


RESULTS
Phagocyte chemiluminescence was higher in patients before haemodialysis compared with that in controls and decreased after haemodialysis compared with predialysis status. A significant increase in plasma antioxidative potential and uric acid was found in patients before haemodialysis. These parameters decreased after haemodialysis compared with both predialysis and control values.


CONCLUSIONS
The higher generation of phagocyte-derived oxidants and the decline in plasma antioxidative properties after haemodialysis confirm insufficient antioxidant defence in patients with chronic renal failure.
Soska
V
V
Department of Clinical Biochemistry and Hematology, St. Ann's University Hospital, Masaryk University. Brno. Czech Republic.
Ciz
M
M
Kubala
L
L
Sobotova
D
D
Lojek
A
A
D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000032:analysis*; D001663:Bilirubin / Q000097:blood; D005260:Female; D005609:Free Radicals / Q000032:analysis; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000628:therapy*; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D016877:Oxidants / Q000097:blood*; D010084:Oxidation-Reduction; D010586:Phagocytes / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000009:adverse effects; D012016:Reference Values; D006435:Renal Dialysis; D012307:Risk Factors; D014527:Uric Acid / Q000097:blood; D014810:Vitamin E / Q000097:blood
D000975:Antioxidants; D005609:Free Radicals; D016877:Oxidants; D017382:Reactive Oxygen Species; D014810:Vitamin E; D014527:Uric Acid; D001663:Bilirubin