title: The phosphorylation pattern of bovine heart complex I subunits.
abstract: The phosphoproteome of bovine heart complex I of the respiratory chain has been analysed with a procedure based on nondenaturing gel electrophoretic separation of complex I from small quantities of mitochondria samples, in-gel digestion, in combination with phosphopeptide enrichment by titanium dioxide and MS. The results, complemented by analyses of purified samples of complex I, showed phosphorylation of five subunits of the complex, 42 kDa (human gene NDUFA10), ESSS, B14.5a (human gene NDUFA7), B14.5b (human gene NDUFC2) and B16.6 (GRIM-19). MS also revealed the presence of phosphorylated programmed cell death protein 8(AIF) in native and purified samples of complex I analysed. The possible physiological relevance of these findings is discussed.
authors: Department of Medical Biochemistry, Biology and Physics, University of Bari, Bari, Italy.
authors: Giuseppe
authors: G
authors: Palmisano
authors: Anna Maria
authors: AM
authors: Sardanelli
authors: Anna
authors: A
authors: Signorile
authors: Sergio
authors: S
authors: Papa
authors: Martin R
authors: MR
authors: Larsen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D030562:Databases, Protein; D042967:Electron Transport Complex I / Q000235:genetics / Q000502:physiology*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010748:Phosphopeptides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D010748:Phosphopeptides; D021122:Protein Subunits; D042967:Electron Transport Complex I