title: Anthrax lethal toxin paralyzes actin-based motility by blocking Hsp27 phosphorylation.
abstract: Inhalation of anthrax causes fatal bacteremia, indicating a meager host immune response. We previously showed that anthrax lethal toxin (LT) paralyzes neutrophils, a major component of innate immunity. Here, we have found that LT also inhibits actin-based motility of the intracellular pathogen Listeria monocytogenes. LT inhibition of actin assembly is mediated by blockade of Hsp27 phosphorylation, and can be reproduced by treating cells with the p38 mitogen-activated protein (MAP) kinase inhibitor SB203580. Nonphosphorylated Hsp27 inhibits Listeria actin-based motility in cell extracts, and binds to and sequesters purified actin monomers. Phosphorylation of Hsp27 reverses these effects. RNA interference knockdown of Hsp27 blocks LT inhibition of Listeria actin-based motility. Rescue with wild-type Hsp27 accelerates Listeria speed in knockdown cells, whereas introduction of Hsp27 mutants incapable of phosphorylation or dephosphorylation causes slowing down. We propose that Hsp27 facilitates actin-based motility through a phosphorylation cycle that shuttles actin monomers to regions of new actin filament assembly. Our findings provide a previously unappreciated mechanism for LT virulence, and emphasize a central role for p38 MAP kinase-mediated phosphorylation of Hsp27 in actin-based motility and innate immunity.
authors: Department of Medicine, University of Florida College of Medicine, Gainesville, FL 32610, USA.
authors: Russell L
authors: RL
authors: During
authors: Bruce G
authors: BG
authors: Gibson
authors: Wei
authors: W
authors: Li
authors: Ellen A
authors: EA
authors: Bishai
authors: Gurjit S
authors: GS
authors: Sidhu
authors: Jacques
authors: J
authors: Landry
authors: Frederick S
authors: FS
authors: Southwick
mesh_terms: D008841:Actin Cytoskeleton / Q000502:physiology; D000199:Actins / Q000378:metabolism*; D000818:Animals; D000942:Antigens, Bacterial / Q000502:physiology* / Q000633:toxicity; D001427:Bacterial Toxins / Q000633:toxicity; D001693:Biological Transport, Active; D001921:Brain / Q000382:microbiology; D016764:Cell Polarity; D055551:HSP27 Heat-Shock Proteins; D006367:HeLa Cells; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D007113:Immunity, Innate; D066298:In Vitro Techniques; D008089:Listeria monocytogenes / Q000187:drug effects / Q000502:physiology*; D018832:Molecular Chaperones; D009154:Mutation; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D009504:Neutrophils / Q000187:drug effects / Q000382:microbiology / Q000502:physiology*; D010766:Phosphorylation; D011725:Pyridines / Q000494:pharmacology; D051381:Rats; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D000199:Actins; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D007093:Imidazoles; D018832:Molecular Chaperones; D009363:Neoplasm Proteins; D011725:Pyridines; C030325:anthrax toxin; D048051:p38 Mitogen-Activated Protein Kinases; C093642:SB 203580