title: MRI monitoring of focal cerebral ischemia in peroxisome proliferator-activated receptor (PPAR)-deficient mice.
abstract: Peroxisome proliferator-activated receptors (PPARs) are a potential target for neuroprotection in focal ischemic stroke. These nuclear receptors have major effects in lipid metabolism, but they are also involved in inflammatory processes. Three PPAR isotypes have been identified: alpha, beta (or delta) and gamma. The development of PPAR transgenic mice offers a promising tool for prospective therapeutic studies. This study used MRI to assess the role of PPARalpha and PPARbeta in the development of stroke. Permanent middle cerebral artery occlusion induced focal ischemia in wild-type, PPARalpha-null mice and PPARbeta-null mice. T(2)-weighted MRI was performed with a 7 T MRI scan on day 0, 1, 3, 7 and 14 to monitor lesion growth in the various genotypes. General Linear Model statistical analysis found a significant difference in lesion volume between wild-type and PPAR-null mice for both alpha and beta isotypes. These data validate high-resolution MRI for monitoring cerebral ischemic lesions, and confirm the neuroprotective role of PPARalpha and PPARbeta in the brain.
authors: Université Lyon 1, Laboratoire CREATIS, INSA de Lyon, CNRS UMR 5515, INSERM U630, Villeurbanne, France.
authors: Jean-Baptiste
authors: JB
authors: Pialat
authors: Tae-Hee
authors: TH
authors: Cho
authors: Olivier
authors: O
authors: Beuf
authors: Elisabeth
authors: E
authors: Joye
authors: Samir
authors: S
authors: Moucharrafie
authors: Samir
authors: S
authors: Moucharaffie
authors: Jean-Baptiste
authors: JB
authors: Langlois
authors: Chantal
authors: C
authors: Nemoz
authors: Marc
authors: M
authors: Janier
authors: Yves
authors: Y
authors: Berthezene
authors: Norbert
authors: N
authors: Nighoghossian
authors: Béatrice
authors: B
authors: Desvergne
authors: Marlène
authors: M
authors: Wiart
mesh_terms: D000818:Animals; D001929:Brain Edema / Q000473:pathology; D002545:Brain Ischemia / Q000139:chemically induced / Q000175:diagnosis* / Q000378:metabolism*; D002544:Cerebral Infarction / Q000473:pathology; D004058:Diffusion; D008279:Magnetic Resonance Imaging; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D047493:PPAR alpha / Q000172:deficiency*; D048068:PPAR-beta / Q000172:deficiency*; D013997:Time Factors
chemical_list: D047493:PPAR alpha; D048068:PPAR-beta