Identification of human hepatocellular carcinoma-related proteins by proteomic approaches.
Hepatocellular carcinoma (HCC) is the most common malignant liver tumor. Analysis of human serum from HCC patients using two-dimensional gel electrophoresis (2DE) combined with nano-high-performance liquid chromatography electrospray ionization tandem mass spectrometry (nano-HPLC-ESI-MS/MS) identified fourteen different proteins differentially expressed between HCC patients and the control group. Twelve proteins were up-regulated and two down-regulated. By using nano-HPLC-MS/MS system to analyze proteome in human serum, 317 proteins were identified, twenty-nine of which to high confidence levels (protein matched at last two unique peptide sequences). Of these twenty-nine proteins, six were present only in HCC patients and may serve as biomarkers for HCC.
Yang
Ming-Hui
MH
Department of Chemistry, Texas Christian University, 2800 S. University Drive, Fort Worth, TX, 76129, USA.
Tyan
Yu-Chang
YC
Jong
Shiang-Bin
SB
Huang
Ying-Fong
YF
Liao
Pao-Chi
PC
Wang
Ming-Chen
MC
D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000097:blood*; D001798:Blood Proteins / Q000032:analysis* / Q000096:biosynthesis; D006528:Carcinoma, Hepatocellular / Q000097:blood* / Q000175:diagnosis; D002851:Chromatography, High Pressure Liquid; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood* / Q000175:diagnosis; D040901:Proteomics / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
D014408:Biomarkers, Tumor; D001798:Blood Proteins