title: Self-reactivities to the non-erythroid alpha spectrin correlate with cerebral malaria in Gabonese children.
abstract: BACKGROUND
  Hypergammaglobulinemia and polyclonal B-cell activation commonly occur in Plasmodium sp. infections. Some of the antibodies produced recognize self-components and are correlated with disease severity in P. falciparum malaria. However, it is not known whether some self-reactive antibodies produced during P. falciparum infection contribute to the events leading to cerebral malaria (CM). We show here a correlation between self-antibody responses to a human brain protein and high levels of circulating TNF alpha (TNFalpha), with the manifestation of CM in Gabonese children.
  
  
  METHODS
  To study the role of self-reactive antibodies associated to the development of P. falciparum cerebral malaria, we used a combination of quantitative immunoblotting and multivariate analysis to analyse correlation between the reactivity of circulating IgG with a human brain protein extract and TNFalpha concentrations in cohorts of uninfected controls (UI) and P. falciparum-infected Gabonese children developing uncomplicated malaria (UM), severe non-cerebral malaria (SNCM), or CM.
  
  
  CONCLUSIONS
  The repertoire of brain antigens recognized by plasma IgGs was more diverse in infected than in UI individuals. Anti-brain reactivity was significantly higher in the CM group than in the UM and SNCM groups. IgG self-reactivity to brain antigens was also correlated with plasma IgG levels and age. We found that 90% of CM patients displayed reactivity to a high-molecular mass band containing the spectrin non-erythroid alpha chain. Reactivity with this band was correlated with high TNFalpha concentrations in CM patients. These results strongly suggest that an antibody response to brain antigens induced by P. falciparum infection may be associated with pathogenic mechanisms in patients developing CM.
authors: Unité d'Immunophysiopathologie Infectieuse, URA CNRS 1961, Université Pierre et Marie Curie Paris, Institut Pasteur, Paris, France.
authors: Vincent
authors: V
authors: Guiyedi
authors: Youri
authors: Y
authors: Chanseaud
authors: Constantin
authors: C
authors: Fesel
authors: Georges
authors: G
authors: Snounou
authors: Jean-Claude
authors: JC
authors: Rousselle
authors: Pharat
authors: P
authors: Lim
authors: Jean
authors: J
authors: Koko
authors: Abdelkader
authors: A
authors: Namane
authors: Pierre-André
authors: PA
authors: Cazenave
authors: Maryvonne
authors: M
authors: Kombila
authors: Sylviane
authors: S
authors: Pied
mesh_terms: D002648:Child; D015331:Cohort Studies; D005681:Gabon; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D016779:Malaria, Cerebral / Q000276:immunology*; D016778:Malaria, Falciparum / Q000276:immunology*; D013058:Mass Spectrometry; D015999:Multivariate Analysis; D013049:Spectrin / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D007074:Immunoglobulin G; D014409:Tumor Necrosis Factor-alpha; D013049:Spectrin