Cytogenetic evaluation of paracetamol effects in human lymphocytes culture.
Paracetamol is a common analgesic and antipyretic drug. It has been recognized as one of the most ordinary medications taken in overdoses. We examined the possible genotoxic effects of high paracetamol concentrations expected to occur after overdose. Paracetamol was added to the cultures at the beginning of the cultivation period. Separate cultures for three tested concentrations of paracetamol (50 microg/mL, 100 microg/mL, and 200 microg/mL) were set. Effects of paracetamol were evaluated by micronucleus cytokinesis-block assay, chromosome aberration analysis, and nuclear division index. Results demonstrate that paracetamol concentration of 200 microg/mL expresses certain genotoxic effects in human peripheral blood lymphocytes.
Ibrulj
Slavka
S
Institute for Genetic Engineering and Biotechnology, University of Sarajevo, Bosnia and Herzegovina. ingeb@ingeb.ba
Rahmanovic
Anisa
A
Haveric
Sanin
S
Haveric
Anja
A
Pasic
Adaleta Durmic
AD
D000082:Acetaminophen / Q000008:administration & dosage / Q000633:toxicity*; D018712:Analgesics, Non-Narcotic / Q000008:administration & dosage / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002842:Chromatids / Q000187:drug effects; D002869:Chromosome Aberrations / Q000139:chemically induced*; D020732:Cytogenetic Analysis; D048749:Cytokinesis / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced; D015162:Micronucleus Tests; D009153:Mutagens / Q000008:administration & dosage / Q000633:toxicity*
D018712:Analgesics, Non-Narcotic; D009153:Mutagens; D000082:Acetaminophen