title: Regulator of G protein signaling proteins differentially modulate signaling of mu and delta opioid receptors.
abstract: Effects of regulator of G protein signaling (RGS) proteins on mu and delta opioid receptors were investigated in HEK293 cells. Co-expression of RGS1, RGS2, RGS4, RGS9, RGS10 or RGS19 (Galpha-interacting protein (GAIP)) significantly reduced [Tyr-D-Ala-Gly-N-methyl-Phe-Gly-ol]-Enkephalin (DAMGO)-induced inhibition of adenylyl cyclase (AC) mediated by mu opioid receptor, but only RGS9 decreased the effects of [Tyr-D-Pen-Gly-p-Chloro-Phe-D-Pen]-Enkephalin (DPDPE) mediated by delta opioid receptor. When C-tails of the receptors were exchanged (mu/deltaC and delta/muC chimeras), RGS proteins decreased delta/muC-mediated AC inhibition, but none had significant effects on that via mu/deltaC receptor. Thus, the C-terminal domains of the receptors are critical for the differential effects of RGS proteins, which may be due to differences in receptor-G protein-RGS protein interactions in signaling complexes.
authors: Department of Pharmacology, School of Basic Medicine and Institute of Basic Medical Sciences, Peking Union Medical College and Chinese Academy of Medical Sciences, 5 Dong Dan San Tiao, Beijing 100005, China.
authors: Zhihua
authors: Z
authors: Xie
authors: Zhisong
authors: Z
authors: Li
authors: Lei
authors: L
authors: Guo
authors: Caiying
authors: C
authors: Ye
authors: Juan
authors: J
authors: Li
authors: Xiaoli
authors: X
authors: Yu
authors: Huifen
authors: H
authors: Yang
authors: Yulin
authors: Y
authors: Wang
authors: Chongguang
authors: C
authors: Chen
authors: Dechang
authors: D
authors: Zhang
authors: Lee-Yuan
authors: LY
authors: Liu-Chen
mesh_terms: D000067956:Adenylyl Cyclase Inhibitors; D000262:Adenylyl Cyclases / Q000378:metabolism; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology; D006801:Humans; D020710:RGS Proteins / Q000502:physiology*; D017465:Receptors, Opioid, delta / Q000502:physiology*; D017450:Receptors, Opioid, mu / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D000067956:Adenylyl Cyclase Inhibitors; D020710:RGS Proteins; D017465:Receptors, Opioid, delta; D017450:Receptors, Opioid, mu; D005576:Colforsin; D000262:Adenylyl Cyclases