A family of poly(U) polymerases.
The GLD-2 family of poly(A) polymerases add successive AMP monomers to the 3' end of specific RNAs, forming a poly(A) tail. Here, we identify a new group of GLD-2-related nucleotidyl transferases from Arabidopsis, Schizosaccharomyces pombe, Caenorhabditis elegans, and humans. Like GLD-2, these enzymes are template independent and add nucleotides to the 3' end of an RNA substrate. However, these new enzymes, which we refer to as poly(U) polymerases, add poly(U) rather than poly(A) to their RNA substrates.
Kwak
Jae Eun
JE
Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA.
Wickens
Marvin
M
D000818:Animals; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000201:enzymology / Q000235:genetics; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics / Q000378:metabolism; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D009713:Nucleotidyltransferases / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism; D010802:Phylogeny; D011062:Polynucleotide Adenylyltransferase / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D012568:Schizosaccharomyces / Q000201:enzymology / Q000235:genetics; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity; D014981:Xenopus
D029742:Caenorhabditis elegans Proteins; D017931:DNA Primers; D012333:RNA, Messenger; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D009713:Nucleotidyltransferases; C022428:poly U polymerase; C520554:Cid1 protein, S pombe; C491056:GLD-2 protein, C elegans; D011062:Polynucleotide Adenylyltransferase; D012321:DNA-Directed RNA Polymerases; C506067:Trf5 protein, S cerevisiae