Analysis of meat samples for anabolic steroids residues by liquid chromatography/tandem mass spectrometry.
A rapid, specific and highly sensitive multi-residue method for the determination of anabolic steroid residues in bovine, pork and poultry muscle tissues was developed. The sample preparation involves enzymatic digestion followed by extraction with methanol. The crude extract was cleaned up by solid-phase extraction (SPE) combining C18 and NH2 columns. The detection was carried out by a highly sensitive liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) method using both positive and negative ionization modes. Natural and synthetic steroids covering different polarities could be extracted, concentrated and purified using one single method. Mobile phase composition and additives were optimized to achieve the highest sensitivity. The linearity was not good enough for quantitative analysis but the method was well-suited for qualitative confirmation. The method was validated according to the European Commission Decision 2002/657/EC. Decision limits (CCalpha) and detection capabilities (CCbeta) were below 0.5 ng g(-1) for all the compounds in the three types of meat studied. The developed method is suitable for routine analysis in our laboratories.
Blasco
Cristina
C
Laboratori de Bromatologia i Toxicologia, Facultat de Farmàcia, Universitat de Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, València, Spain. cristina.blasco@uv.es
Van Poucke
Christof
C
Van Peteghem
Carlos
C
D045930:Anabolic Agents / Q000032:analysis*; D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods*; D008460:Meat / Q000032:analysis*; D009132:Muscles / Q000737:chemistry; D011200:Poultry; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods*; D035501:Uncertainty
D045930:Anabolic Agents