Improvement of the quality of boar cryopreservation semen by supplementing with low density lipoprotein in diluents.
Low density lipoprotein was added at concentrations of 6%, 7%, 8%, 9% and 10% to the diluents used to freeze boar semen and its effect on the quality of cryopreservation was assessed The results indicated that 9% low density lipoprotein supplementation significantly improved the total motile sperm (p<0.05). The sperm straight-line velocity increased with the concentration of low density lipoprotein except at the concentration of 10%, at which concentration the sperm velocity declined (p<0.05). Supplementation at 8% and 9% low density lipoprotein significantly increased plasma membrane integrity (p<0.05). Compared with control, the low density lipoprotein supplementation significantly improved the percentage of acrosome integrity (p<0.05). With all parameters measured, the concentration of 9% low density lipoprotein showed a better effect on the quality of boar cryopreservation semen. The sperm DNA was more seriously damaged in the species of Yorkshire than in Duroc.
Jiang
Zhong-liang
ZL
College of Animal Science & Technology, Northwest A&F University, Yangling, ShaanXi Province 712100, PR China. jiangzhongliang73@yahoo.com.cn
Li
Qing-wang
QW
Hu
Jian-hong
JH
Li
Wen-ye
WY
Zhao
Hong-wei
HW
Zhang
Shu-shan
SS
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods*; D004249:DNA Damage / Q000187:drug effects; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D008297:Male; D012661:Semen / Q000187:drug effects*; D012662:Semen Preservation / Q000379:methods*; D013045:Species Specificity; D013552:Swine
D008077:Lipoproteins, LDL