Characteristic of hypothalamic kisspeptin expression in the pubertal development of precocious female rats.
To investigate the potential role of kisspeptin in the advance onset of puberty in precocious puberty, model rats induced by danazol were used to study the developmental expression of hypothalamic kisspeptin. Kisspeptin immunoreactive cells were observed in the arcuate nucleus (ARC), periventricular nucleus (PeN) and preoptic area (POA) in model rats on the day of onset-puberty. On the day of post-puberty, however, the number of kisspeptin immunoreactive cells in ARC and PeN decreased while the number of those cells in POA increased. Kisspeptin immunoreactive cells were not detected in hypothalamus in both normal and model rats at their pre-puberty stages. Furthermore, the expression of hypothalamic Kiss-1 mRNA reached top on the day of onset-puberty in both of the normal and model rats, and the expression of Kiss-1 mRNA increased significantly in the model rats compared with those in the normal ones. Our results indicated that kisspeptin might involve in the advance onset of puberty in danazol induced female precocious model rats.
Sun
Yan
Y
Department of Neurobiology and Integrative Medicine, Shanghai Medical College of Fudan University (Formerly Shanghai Medical University), P.O. Box 291, 138 Yi-Xue-Yuan Road, 200032 Shanghai, PR China.
Tian
Zhanzhuang
Z
Zhao
Hong
H
Wong
Stephen T C
ST
Chen
Boying
B
D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000254:growth & development / Q000378:metabolism; D003613:Danazol / Q000494:pharmacology*; D003720:Densitometry; D004965:Estrogen Antagonists / Q000494:pharmacology; D030762:Estrous Cycle / Q000187:drug effects; D005260:Female; D007031:Hypothalamus / Q000378:metabolism*; D059648:Kisspeptins; D020644:Midline Thalamic Nuclei / Q000254:growth & development / Q000378:metabolism; D010053:Ovary / Q000254:growth & development; D011301:Preoptic Area / Q000254:growth & development / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012741:Sexual Maturation / Q000502:physiology*; D014599:Uterus / Q000254:growth & development; D014621:Vagina / Q000254:growth & development
D004965:Estrogen Antagonists; C491406:Kiss1 protein, rat; D059648:Kisspeptins; D011506:Proteins; D012333:RNA, Messenger; D003613:Danazol