Transfection and expression of plasmid DNA in plant cells by an arginine-rich intracellular delivery peptide without protoplast preparation.
The delivery and expression of exogenous genes in plant cells have been of particular interest for plant research and biotechnology. Here, we present results demonstrating a simple DNA transfection system in plants. Short arginine-rich intracellular delivery peptide, a protein transduction domain, was capable of delivering plasmid DNA into living plant cells non-covalently. This peptide-mediated DNA delivery conferred several advantages, such as nuclear targeting, non-toxic effect, and ease of preparation without protoplast formulation. Thus, this novel technology shall provide a powerful tool to investigate gene function in vivo, and lay the foundation for the production of transgenic plants in future.
Chen
Chung-Pin
CP
Department of Life Science, National Dong Hwa University, Hualien 97401, Taiwan.
Chou
Jyh-Ching
JC
Liu
Betty Revon
BR
Chang
Microsugar
M
Lee
Han-Jung
HJ
D001120:Arginine / Q000737:chemistry; D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors / Q000737:chemistry; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D010944:Plants / Q000235:genetics*; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000378:metabolism; D011523:Protoplasts / Q000378:metabolism*; D013997:Time Factors; D014162:Transfection / Q000379:methods*
D010455:Peptides; D001120:Arginine