title: Establishment of autologous embryonic stem cells derived from preantral follicle culture and oocyte parthenogenesis.
abstract: OBJECTIVE
  To evaluate whether autologous embryonic stem cells can be established without generating clone embryos.
  
  
  METHODS
  Prospective model study.
  
  
  METHODS
  Gamete and stem cell biotechnology laboratory in Seoul National University, Seoul, Korea.
  
  
  METHODS
  F1 hybrid B6D2F1 mice.
  
  
  METHODS
  Preantral follicles were cultured, and oocytes matured in the follicles were parthenogenetically activated.
  
  
  METHODS
  Preimplantation development and stem cell characterization.
  
  
  RESULTS
  More intrafollicular oocytes that were retrieved from secondary follicles matured and developed into blastocysts after parthenogenesis than those that were retrieved from primary follicles. Of those 35 blastocysts derived from 193 parthenotes, one line of colony-forming cells was established from the culturing of early secondary follicles. The established cells were positive for embryonic stem cell-specific markers and had normal diploid karyotype and telomerase activity. They differentiated into embryoid bodies in vitro and teratomas in vivo. Inducible differentiation of the established cells into neuronal lineage cells also was possible.
  
  
  CONCLUSIONS
  Autologous embryonic stem cells can be established by preantral follicle culture and oocyte parthenogenesis. A combined technique of follicle culture and oocyte parthenogenesis that does not use developmentally competent oocytes has the potential to replace somatic cell nuclear transfer for autologous cell therapy.
authors: Department of Agricultural Biology, Gamete and Stem Cell Biotechnology Laboratory, Seoul National University, Seoul, Korea.
authors: Seung Tae
authors: ST
authors: Lee
authors: Mun Hwan
authors: MH
authors: Choi
authors: Eun Ju
authors: EJ
authors: Lee
authors: Seung Pyo
authors: SP
authors: Gong
authors: Mi
authors: M
authors: Jang
authors: Sang Hyun
authors: SH
authors: Park
authors: Hyang
authors: H
authors: Jee
authors: Dae Yong
authors: DY
authors: Kim
authors: Jae Yong
authors: JY
authors: Han
authors: Jeong Mook
authors: JM
authors: Lim
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000502:physiology; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002460:Cell Line; D019070:Cell Lineage; D049109:Cell Proliferation; D053595:Embryonic Stem Cells / Q000502:physiology* / Q000637:transplantation; D005240:Feasibility Studies; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D009865:Oocytes / Q000502:physiology*; D006080:Ovarian Follicle / Q000166:cytology / Q000502:physiology*; D010312:Parthenogenesis; D033581:Stem Cell Transplantation; D013724:Teratoma / Q000473:pathology; D046509:Tissue Culture Techniques; D014182:Transplantation, Autologous