title: Oxidative stress and protease dysfunction in the yeast model of Friedreich ataxia.
abstract: Friedreich ataxia has frequently been associated with an increased susceptibility to oxidative stress. We used the yeast (Saccharomyces cerevisiae) model of Friedreich ataxia to study the physiological consequences of a shift from anaerobiosis to aerobiosis. Cells lacking frataxin (Deltayfh1) showed no growth defect when cultured anaerobically. Under these conditions, a significant amount of aconitase was functional, with an intact 4 Fe/4 S cluster. When shifted to aerobic conditions, aconitase was rapidly degraded, and oxidatively modified proteins (carbonylated and HNE-modified proteins) accumulated in both the cytosol and the mitochondria. The ATP-dependent mitochondrial protease Pim1 (Lon) was strongly activated, although its expression level remained unchanged, and the cytosolic activity of the 20S proteasome was greatly decreased, compared to that in wild-type cells. Analysis of the purified proteasome revealed that the decrease in proteasome activity was likely due to both direct inactivation of the enzyme and inhibition by cytosolic oxidized proteins. These features indicate that the cells were subjected to major oxidative stress triggered by oxygen. Accumulation of oxidatively modified proteins, activation of Pim1, and proteasome inhibition did not directly depend on the amount of mitochondrial iron, because these phenotypes remained unchanged when the cells were grown under iron-limiting conditions, and these phenotypes were not observed in another mutant (Deltaggc1) which overaccumulates iron in its mitochondrial compartment. We conclude that oxygen is primarily involved in generating the deleterious phenotypes that are observed in frataxin-deficient yeast cells.
authors: Laboratoire de Biologie et Biochimie Cellulaire du Vieillissement, Universite Paris 7, Paris, France.
authors: Anne-Laure
authors: AL
authors: Bulteau
authors: Andrew
authors: A
authors: Dancis
authors: Monique
authors: M
authors: Gareil
authors: Jean-Jacques
authors: JJ
authors: Montagne
authors: Jean-Michel
authors: JM
authors: Camadro
authors: Emmanuel
authors: E
authors: Lesuisse
mesh_terms: D049069:ATP-Dependent Proteases; D004789:Enzyme Activation; D005621:Friedreich Ataxia / Q000201:enzymology* / Q000235:genetics; D017353:Gene Deletion; D007501:Iron / Q000032:analysis / Q000378:metabolism; D033862:Iron-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000737:chemistry / Q000378:metabolism; D024101:Mitochondrial Proteins; D008954:Models, Biological; D018384:Oxidative Stress / Q000235:genetics; D010100:Oxygen / Q000378:metabolism / Q000494:pharmacology; D010641:Phenotype; D046988:Proteasome Endopeptidase Complex / Q000235:genetics / Q000378:metabolism; D061988:Proteasome Inhibitors; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D012697:Serine Endopeptidases / Q000378:metabolism; D000096923:Frataxin
chemical_list: D049069:ATP-Dependent Proteases; D007501:Iron; D033862:Iron-Binding Proteins; D024101:Mitochondrial Proteins; D010100:Oxygen; D046988:Proteasome Endopeptidase Complex; D061988:Proteasome Inhibitors; D029701:Saccharomyces cerevisiae Proteins; D012697:Serine Endopeptidases; D000096923:Frataxin; C085155:PIM1 protein, S cerevisiae