Itaconic acid production using sago starch hydrolysate by Aspergillus terreus TN484-M1.
Sago starch was hydrolyzed using either chemical agents, or enzymes at various pH and concentrations. Hydrolysis using 5000 AUN/ml (0.5%, w/v) glucoamylase exhibited the highest itaconic acid yield up to 0.36 g/g sago starch, whereas hydrolysis using nitric acid at pH 2.0 yielded 0.35 g/g sago starch. The medium was optimized and the composition was (g/l) 140 sago starch, 1.8 corn steep liquor, 1.2 MgSO(4).7H(2)O and 2.9 NH(4)NO(3). When the optimal conditions of hydrolysis and medium composition were applied to itaconic acid production in a 3-l jar fermentor, the itaconic acid production was 48.2 g/l with a yield of 0.34 g/g sago starch. This was filtered from the cultured broth and 37.1g of itaconic acid was recovered with a purity of 97.2%. This result showed that sago starch could be converted to a value-added product with only a simple pretreatment.
Dwiarti
Lies
L
Science of Biological Resources, The United Graduate School of Agricultural Science, Gifu University, 1-1 Yanagido, Gifu, Japan.
Otsuka
Megumi
M
Miura
Shigenobu
S
Yaguchi
Masaaki
M
Okabe
Mitsuyasu
M
D028023:Arecaceae / Q000737:chemistry*; D001230:Aspergillus / Q000378:metabolism*; D005285:Fermentation; D013213:Starch / Q000737:chemistry / Q000378:metabolism*; D013386:Succinates / Q000378:metabolism*
D013386:Succinates; D013213:Starch; C005229:itaconic acid