Characterization of AP3B2_v2, a novel splice variant of human AP3B2.
A novel splice variant of human AP3B2, named AP3B2_v2, was isolated through the large-scale sequencing analysis of a human fetal brain cDNA library. The AP3B2_v2 cDNA is 1171 bp in length. Sequence analysis revealed AP3B2_v2 missed 22 exons that existed in AP3B2_v1, leading to a different putative protein. The deduced proteins were 145 amino acids (designated as AP3B2_v2) and 1082 amino acids (AP3B2_v1) in length, sharing the C-terminal 145 amino acids. RT-PCR analysis showed that human AP3B2_v2 were expressed in several human adult tissues analyzed. The expression levels of AP3B2_v2 were relatively high in brain and testis. In contrast, low levels of expression were detected in kidney, pancreas, spleen, thymus, prostate, ovary and small intestine.
Chen
Chunjing
C
State Key Laboratory of Genetic Engineering, School of Life Sciences, Institute of Genetics, Fudan University, Shanghai, 200433, People's Republic of China.
Zou
Xianqiong
X
Ji
Chaoneng
C
Zhao
Shuo
S
Lv
Lei
L
Gu
Shaohua
S
Xie
Yi
Y
Mao
Yumin
Y
D033963:Adaptor Protein Complex 3 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D033966:Adaptor Protein Complex beta Subunits / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D005333:Fetus / Q000378:metabolism; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D009928:Organ Specificity; D020133:Reverse Transcriptase Polymerase Chain Reaction
C095597:AP3B2 protein, human; D033963:Adaptor Protein Complex 3; D033966:Adaptor Protein Complex beta Subunits