Evolutionary formation of new centromeres in macaque.
A systematic fluorescence in situ hybridization comparison of macaque and human synteny organization disclosed five additional macaque evolutionary new centromeres (ENCs) for a total of nine ENCs. To understand the dynamics of ENC formation and progression, we compared the ENC of macaque chromosome 4 with the human orthologous region, at 6q24.3, that conserves the ancestral genomic organization. A 250-kilobase segment was extensively duplicated around the macaque centromere. These duplications were strictly intrachromosomal. Our results suggest that novel centromeres may trigger only local duplication activity and that the absence of genes in the seeding region may have been important in ENC maintenance and progression.
Ventura
Mario
M
Department of Genetics and Microbiology, University of Bari, 70126 Bari, Italy.
Antonacci
Francesca
F
Cardone
Maria Francesca
MF
Stanyon
Roscoe
R
D'Addabbo
Pietro
P
Cellamare
Angelo
A
Sprague
L James
LJ
Eichler
Evan E
EE
Archidiacono
Nicoletta
N
Rocchi
Mariano
M
D000818:Animals; D001483:Base Sequence; D002503:Centromere; D002896:Chromosomes, Human, Pair 6; D004247:DNA; D019143:Evolution, Molecular; D020440:Gene Duplication; D006801:Humans; D008253:Macaca mulatta / Q000235:genetics*; D008969:Molecular Sequence Data; D016324:Sequence Tagged Sites; D026801:Synteny
D004247:DNA