Preparation and characterization of standard reference material 3276, carrot extract in oil.
The National Institute of Standards and Technology (NIST), the Food and Drug Administration (FDA) Center for Drug Evaluation and Research (CDER) and Center for Food Safety and Applied Nutrition (CFSAN), and the National Institutes of Health (NIH), Office of Dietary Supplements (ODS) are collaborating to produce a series of standard reference materials (SRMs) for dietary supplements. Standard reference material (SRM) 3276 Carrot Extract in Oil is one in this series, with values assigned for trans-alpha-carotene, trans- and total beta-carotene, delta- and gamma-tocopherol, and twelve fatty acids. Results for carotenoids and tocopherols were obtained by use of combinations of liquid chromatography (LC), on columns of differing selectivity, with absorbance and mass spectrometric (MS) detection. Fluorescence detection also was used for the tocopherols. Results for fatty acids were obtained by use of gas chromatography (GC) with both flame-ionization and mass-spectrometric detection. This material is intended for use as a primary control material when assigning values to in-house (secondary) control materials and for validation of analytical methods for measurement of these analytes in similar matrices.
Sharpless
Katherine E
KE
Analytical Chemistry Division, National Institute of Standards and Technology, Gaithersburg, MD 20899-8392, USA. katherine.sharpless@nist.gov
Thomas
Jeanice Brown
JB
Duewer
David L
DL
Putzbach
Karsten
K
Rimmer
Catherine A
CA
Sander
Lane C
LC
Schantz
Michele M
MM
Wise
Stephen A
SA
Yarita
Takashi
T
Yen
James H
JH
D002338:Carotenoids / Q000737:chemistry; D002849:Chromatography, Gas; D002853:Chromatography, Liquid; D018552:Daucus carota / Q000737:chemistry*; D004952:Esters / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D008745:Methylation; D010936:Plant Extracts / Q000737:chemistry*; D010938:Plant Oils / Q000032:analysis* / Q000737:chemistry / Q000592:standards*; D012015:Reference Standards; D024505:Tocopherols / Q000737:chemistry; D037041:United States Government Agencies
D004952:Esters; D005227:Fatty Acids; D010936:Plant Extracts; D010938:Plant Oils; D002338:Carotenoids; D024505:Tocopherols