Production and regulation of lipase activity from Penicillium restrictum in submerged and solid-state fermentations.
Different carbon (C) sources, mainly carbohydrates and lipids, have been screened for their capacity to support growth and lipase production by Penicillium restrictum in submerged fermentation (SmF) and in solid-state fermentation (SSF). Completely different physiological behaviors were observed after the addition of easily (oleic acid and glucose) and complex (olive oil and starch) assimilable C sources to the liquid and solid media. Maximal lipolytic activities (12.1 U/mL and 17.4 U/g) by P. restrictum were obtained with olive oil in SmF and in SSF, respectively. Biomass levels in SmF (12.2-14.1 mg/mL) and SSF (7.0-8.0 mg/g) did not varied greatly with the distinct C sources used. High lipase production (12.3 U/g) using glucose was only attained in SSF, perhaps due to the ability of this fermentation process to minimize catabolite repression.
de Azeredo
Luciana A I
LA
Biochemistry Department, Institute of Chemistry, Federal University of Rio de Janeiro, Centro de Tecnologia, Bloco A, Lab. 549-2, 21945-900 Rio de Janeiro, RJ, Brazil.
Gomes
Patrícia M
PM
Sant'Anna
Geraldo L
GL
Castilho
Leda R
LR
Freire
Denise M G
DM
D004789:Enzyme Activation / Q000187:drug effects; D005285:Fermentation / Q000187:drug effects / Q000502:physiology*; D005947:Glucose / Q000494:pharmacology; D008049:Lipase / Q000378:metabolism*; D019301:Oleic Acid / Q000494:pharmacology; D000069463:Olive Oil; D010407:Penicillium / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D010938:Plant Oils / Q000494:pharmacology; D013213:Starch / Q000494:pharmacology
D000069463:Olive Oil; D010938:Plant Oils; D019301:Oleic Acid; D013213:Starch; D008049:Lipase; D005947:Glucose