title: [Anti-tumor effect of adenovirus vector generated from homologous recombination of bacteria mediating TK gene on hepatic neoplasm cell].
abstract: OBJECTIVE
  To test the anti-tumor effect of adenovirus vector generated from the homologous recombination of bacteria mediating TK gene on hepatic neoplasm cell.
  
  
  METHODS
  TK gene was liberated from plasmid and subcloned into shuttle plasmid, which formed the plasmid of pAdTrack-GFP-TK. The pAdTrack-GFP-TK plasmid was then linealized with Pme I and cotransformed into BJ5183 bacterial cells with adenovirus genomic DNA plasmid of pAdEasy-1. The recombinant adenovirus plasmid DNA was digested with Pac I and transfected to the 293 cells to package the recombinant adenovirus particles. The infection rate of the recombinant adenovirus, the target gene expression in the carcinoma cells, the survival rate of the GCV treated hepatic neoplasm cell SMMC-7721 infected with recombinant adenovirus, and the bystander effect were measured.
  
  
  RESULTS
  The titre of purified Ad was 2 x 10(12) efu/mL. More than 90% of the SMMC-7721 cells were infected when the MOI reached 100. Target gene expression was detected in the infected cells. The SMMC-7721 cells infected with AdEasy-GFP-TK were very sensitive to the prodrug GCV, with IC50 less than 0.2 pmol/L. Significant bystander effect was observed.
  
  
  CONCLUSIONS
  The constructed AdEasy-GFP-TK virus can efficiently infect the hepatic neoplasm cells and enable the SMMC-7721 cells to express the HSV-TK genes. The bystander effect has improved the anti-tumor effect of the TK gene, which makes the clinical application possible.
authors: Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
authors: Zi-Ming
authors: ZM
authors: Liu
authors: Lü-Nan
authors: LN
authors: Yan
mesh_terms: D000256:Adenoviridae / Q000235:genetics* / Q000502:physiology; D001419:Bacteria / Q000235:genetics*; D024201:Bystander Effect / Q000187:drug effects / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004274:DNA, Recombinant / Q000235:genetics*; D015774:Ganciclovir / Q000494:pharmacology; D005818:Genetic Engineering / Q000379:methods*; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology* / Q000628:therapy / Q000821:virology; D010957:Plasmids / Q000235:genetics; D018139:Simplexvirus / Q000201:enzymology; D013937:Thymidine Kinase / Q000235:genetics*; D019562:Viral Load
chemical_list: D004274:DNA, Recombinant; D013937:Thymidine Kinase; D015774:Ganciclovir