PKD1 inhibits cancer cells migration and invasion via Wnt signaling pathway in vitro.
The approximately 14 kb mRNA of the polycystic kidney disease gene PKD1 encodes a large ( approximately 460 kDa) protein, termed polycystin-1 (PC-1), that is responsible for autosomal dominant polycystic kidney disease (ADPKD). The unique organization of its multiple adhesive domains (16 Ig-like domains/PKD domains) suggests that it may play an important role in cell-cell/cell-matrix interactions. Here we demonstrated that PKD1 promoted cell-cell and cell-matrix interactions in cancer cells, indicating that PC-1 is involved in the cell adhesion process. Furthermore in this study, we showed that PKD1 inhibited cancer cells migration and invasion. And we also showed that PC-1 regulated these processes in a process that may be at least partially through the Wnt pathway. Collectively, our data suggest that PKD1 may act as a novel member of the tumor suppressor family of genes.
Zhang
Ke
K
State Key Laboratory of Biotherapy and Cancer Center, west China Medical School, and School of Life Science, Sichuan University, Chengdu 610041, China.
Ye
Chun
C
Zhou
Qin
Q
Zheng
Rong
R
Lv
Xiaoyan
X
Chen
Ye
Y
Hu
Zhongguo
Z
Guo
Hong
H
Zhang
Zheng
Z
Wang
Yidong
Y
Tan
Ruizhi
R
Liu
Yuhang
Y
D015153:Blotting, Western; D015820:Cadherins / Q000378:metabolism; D002448:Cell Adhesion / Q000235:genetics / Q000502:physiology; D002449:Cell Aggregation / Q000235:genetics / Q000502:physiology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics / Q000502:physiology*; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D006023:Glycoproteins / Q000494:pharmacology; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D009361:Neoplasm Invasiveness; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D050396:TRPP Cation Channels / Q000378:metabolism*; D014162:Transfection; D051155:Wnt1 Protein / Q000037:antagonists & inhibitors / Q000502:physiology*; D051176:beta Catenin / Q000378:metabolism; D000099274:Frizzled-Related Proteins
D015820:Cadherins; D018076:DNA, Complementary; D006023:Glycoproteins; D047908:Intracellular Signaling Peptides and Proteins; D050396:TRPP Cation Channels; D051155:Wnt1 Protein; D051176:beta Catenin; D000099274:Frizzled-Related Proteins