Cytokine expression in the intestine of rainbow trout (Oncorhynchus mykiss) during infection with Aeromonas salmonicida.
Gene expression of a number of cytokines in the intestine of rainbow trout (Oncorhynchus mykiss) was investigated after challenge with a pathogenic strain of Aeromonas salmonicida. Fish were exposed to A. salmonicida by immersion in a bacterial suspension (bath challenge) and tissue samples of the distal and proximal intestine were collected at days 0, 2, 4, 6 and 8 post-exposure. Head kidney tissue was also collected to assess the effect in a systemic immune tissue. A classic profile of pro-inflammatory cytokine upregulation was observed in the proximal intestine of fish infected by bath challenge, as determined by semi-quantitative RT-PCR. Expression of IL-1beta, IL-8, TNF-alpha and IFN-gamma was increased in the proximal intestine. TGF-beta was significantly decreased in the distal intestine. In the head kidney, infection with A. salmonicida by bath challenge caused decreased expression levels of IL-1beta, IL-8, TNF-alpha and TGF-beta. The results are discussed in the context of potential immune mechanisms in the gut to prevent infection.
Mulder
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IE
Scottish Fish Immunology Research Centre, University of Aberdeen, Aberdeen AB24 2TZ, Scotland, UK.
Wadsworth
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S
Secombes
C J
CJ
D048409:Aeromonas salmonicida / Q000276:immunology*; D000818:Animals; D016207:Cytokines / Q000235:genetics*; D005393:Fish Diseases / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D005786:Gene Expression Regulation / Q000276:immunology*; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000662:veterinary*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000276:immunology / Q000382:microbiology; D017686:Oncorhynchus mykiss / Q000235:genetics* / Q000276:immunology / Q000382:microbiology*
D016207:Cytokines