Identification of exocytotic membrane proteins, syntaxin-1 and SNAP-25, in Entamoeba histolytica from hamster liver.
Entamoeba histolytica is a protozoan parasite causing dysentery and in some cases liver abscesses. These effects have been attributed to cytolytic substances released by exocytosis. In this study, the presence of the proteins syntaxin-1 and SNAP-25, which are assumed to be involved in exocytosis, were examined by immunohistochemistry, immunoelectron microscopy and western blot analysis. Syntaxin-1 and SNAP-25 were expressed in the vesicular, vacuolar and plasma membranes of E. histolytica trophozoites. It can be concluded that these proteins might be involved in exocytosis processes.
Ventura-Juárez
Javier
J
Departments of Morphology, Center of Basic Sciences, Aguascalientes Autonomous University, Aguascalientes, Mexico City, Mexico.
Salinas
Eva
E
Campos-Rodríguez
Rafael
R
Kouri
Juan B
JB
Quintanar
Luis
L