Synthesis and characterization of a molecularly imprinted silica gel sorbent for the on-line determination of trace Sudan I in Chilli powder through high-performance liquid chromatography.
A highly selective imprinted polymer was synthesized by a surface molecular imprinting technique in combination with a sol-gel process. The imprinted polymer was evaluated by FT-IR and static and kinetic adsorption experiments. The results showed that the imprinted sorbent exhibited good recognition and selective ability, offered a faster kinetics for the adsorption and desorption of Sudan I than the non-imprinted sorbent, a saturated binding capacity (Qmax) that reached 33.47 mg g-1. The prepared sorbent was applied for the determination of trace Sudan I through on-line solid-phase coupled with high-performance liquid chromatography (SPE-HPLC). With a loading flow rate of 1.5 mL min-1 for sampling 50 mL, an enrichment factor of 1266 was achieved. The detection limit (S/N = 3) was 1.2 ng L-1, and the peak area precision (RSD) for five replicate detections of 0.01 microg L-1 Sudan I was 3.66%. The Sudan I in the chilli powder from the local market was determined at three levels (0.25, 0.5, and 1.0 ng g-1) with recoveries ranging from 80.31 to 94.02%.
Wang
Shuo
S
Tianjin Key Laboratory of Food Nutrition and Safety, Faculty of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, People's Republic of China. s.wang@tust.edu.cn
Xu
Zhixiang
Z
Fang
Guozhen
G
Duan
Zhenjuan
Z
Zhang
Yan
Y
Chen
Shuang
S
D000327:Adsorption; D002212:Capsicum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004396:Coloring Agents / Q000032:analysis*; D005519:Food Preservation; D009284:Naphthols / Q000032:analysis*; D058428:Silica Gel; D012822:Silicon Dioxide
D004396:Coloring Agents; D009284:Naphthols; C024336:1-phenylazo-2-naphthol; D058428:Silica Gel; D012822:Silicon Dioxide