title: Development of a novel glue consisting of naturally-derived biomolecules: citric acid and human serum albumin.
abstract: A novel glue consisting of human serum albumin (HSA) and citric acid derivative (CAD) was developed where the glue is named as CAD-A glue. In this adhesive, CAD works as a crosslinking reagent of HSA. For preparing crosslinking reagent CAD, using citric acid as a starting material, three carboxyl groups of a citric acid were modified with N-hydroxysuccinimide in the presence of 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride. From 1H-NMR spectrum, CAD with three active ester groups in a molecule was successfully synthesized with a high yield (more than 80%). The boding time of CAD-A glue to collagen-based casing was saturated within 15 minutes. The bonding strength of this glue to collagen-based casings increased with increasing of HSA concentration. The maximum bonding strength of CAD-A glue was a slightly low level compared to the bonding strength of cyanoacrylate adhesive and was 9 times higher than that of fibrin glue. The CAD-A glue showed excellent biocompatibility and high ability of wound closure similar to that of cyanoacrylate-based adhesive when glues were applied to the mouse skin. These results suggested that this developed adhesive had both tissue compatibility and bonding strength for use in clinical field.
authors: Biomaterials Center National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan.
authors: Tetsushi
authors: T
authors: Taguchi
authors: Hirofumi
authors: H
authors: Saito
authors: Masashi
authors: M
authors: Iwasashi
authors: Masataka
authors: M
authors: Sakane
authors: Sachiro
authors: S
authors: Kakinoki
authors: Naoyuki
authors: N
authors: Ochiai
authors: Junzo
authors: J
authors: Tanaka
mesh_terms: D000269:Adhesives / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D019343:Citric Acid / Q000737:chemistry; D003487:Cyanoacrylates; D015718:Fibrin Tissue Adhesive; D008422:Materials Testing; D051379:Mice; D036103:Nanotechnology; D012709:Serum Albumin / Q000737:chemistry; D012867:Skin / Q000293:injuries / Q000473:pathology; D014014:Tissue Adhesives / Q000737:chemistry*
chemical_list: D000269:Adhesives; D001672:Biocompatible Materials; D003487:Cyanoacrylates; D015718:Fibrin Tissue Adhesive; D012709:Serum Albumin; D014014:Tissue Adhesives; D019343:Citric Acid