SMYD3 tandem repeats polymorphism is not associated with the occurrence and metastasis of hepatocellular carcinoma in a Chinese population.
A variable number of tandem repeats (VNTR) polymorphism in regulatory region of SMYD3 coding for histone methyltransferase has been shown to be associated with colorectal cancer, hepatocellular carcinoma (HCC), and breast cancer in Japanese population. Aim of the study is to investigate the potential association between the functional SMYD3 tandem repeats polymorphism and HCC in Chinese population.


METHODS
The case-control study included 200 HCC patients and 261 healthy controls. The VNTR polymorphism in the promoter of SMDY3 was genotyped by PCR and direct-sequencing analysis. Odds ratio and 95% confidence interval were used to estimate the association between the polymorphisms and risk of HCC.


RESULTS
The allele frequencies for SMYD3 2 and 3 repeats were 15.71% and 84.29% among controls; and 12.75%, and 87.25% among cases (P = 0.22). The odds ratio for 3/3 versus 2/2 and 2/3 genotypes was 1.30 (P = 0.18). The frequencies of 3 alleles were not increased with HCC stage increased (trend test, P = 0.45).


CONCLUSIONS
SMYD3 polymorphism is not associated with the occurrence and metastasis of HCC in Chinese population.
Wang
X Q
XQ
Department of Surgery, The University of Hong Kong, Pokfulam, Hong Kong. xqwang@hkucc.hku.hk
Miao
X
X
Cai
Q
Q
Garcia-Barcelo
M M
MM
Fan
S T
ST
D000328:Adult; D044466:Asian People; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000556:secondary; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D011495:Histone-Lysine N-Methyltransferase / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000378:metabolism; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012307:Risk Factors; D020080:Tandem Repeat Sequences / Q000235:genetics*
D011495:Histone-Lysine N-Methyltransferase; C506278:SMYD3 protein, human