[Effects of L. lactis recombinant heme oxygenase-1 gene on the intestinal barrier in rats with hemorrhagic shock].
OBJECTIVE
To evaluate the effect of gavage of L. lactis recombinant heme oxygenase-1 (HO-1) gene on protection of the intestinal mucosa and the inflammation of lower intestine during hemorrhagic shock.


METHODS
A model of hemorrhagic shock was reproduced in 30 SD healthy male rats. They were randomly divided into the L. lactis recombinant HO-1 gene group (HO group, n=10), L. lactis group (LL group, n=10), and glutamine group (Glu group, n=10). Glu was gavaged 6 hours and other agents were gavaged 24 hours before the experiment. Samples were collected 1 hour after hemorrhagic shock and fluid resuscitation. The mortality, mean arterial pressure (MAP) during hemorrhagic shock and fluid resuscitation, myeloperoxidase (MPO) activity, the pathological changes, and the contents of HO-1, tumor necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-10) of the lower intestine were determined immunohistochemically and compared.


RESULTS
When the results of HO group were compared with Glu and LL groups, the mortality was significantly decreased in the former (both P<0.05). In HO group, MAP values at 5, 10, 20 and 30 minutes after fluid resuscitation were significantly elevated (all P<0.05). Compared with LL group, the gray levels of IL-10 and HO-1 in HO group and Glu group were significantly increased (all P<0.05). Compared with Glu group, the gray level of HO-1 was significantly increased in HO group (P<0.05). There were no significant differences in the gray levels of TNF-alpha among three groups. The Chiu's grade of HO group [(1.41+/-0.28) scores] was significantly lower than those of LL group and Glu group [(1.93+/-0.49) scores and (1.75+/-0.58) scores, respectively, both P<0.05].


CONCLUSIONS
The L. lactis recombinant HO-1 has the virtue to deliver HO-1 activity in rats with hemorrhagic shock, which is beneficial for the maintenance of intestinal barrier and anti-inflammation response of the lower intestine.
Gao
Xin-Yue
XY
Department of Anesthesiology, The Fourth People's Hospital of Shenzhen, Shenzhen, Guangdong, China. gaodays@163.com
Ren
Cong-Cai
CC
Zhang
Xin
X
Yao
Yue-Hua
YH
Pang
Qing-Feng
QF
Wu
Chang-Yi
CY
D000818:Animals; D004195:Disease Models, Animal; D051547:Heme Oxygenase-1 / Q000235:genetics* / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000473:pathology*; D013294:Lactococcus lactis / Q000235:genetics*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012771:Shock, Hemorrhagic / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D051547:Heme Oxygenase-1