Combined supercritical fluid chromatographic methods for the characterization of octadecylsiloxane-bonded stationary phases.
In this paper, we present a combination of a key-solute test based on retention and separation factors of large probe solutes (carotenoid pigments) and a quantitative structure-retention relationship analysis based on the retention factors of small probe solutes (aromatic compounds) to investigate the different chromatographic behavior of octadecylsiloxane-bonded stationary phases of all sorts: classical, protected against silanophilic interactions or not, containing polar groups (endcapping groups or embedded groups). Varied chemometric methods are used to enlighten the differences between the 27 phases tested. The results indicate that the two approaches chosen (carotenoid test and solvation parameter model) are complementary and provide precise information on the chromatographic behavior of ODS phases.
Lesellier
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Groupe de Chimie Analytique de Paris-Sud, EA 4041, IUT d'Orsay, 91400 Orsay, France. eric.lesellier@univ-orleans.fr
West
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C
D002338:Carotenoids / Q000302:isolation & purification; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D015335:Molecular Probes; D021281:Quantitative Structure-Activity Relationship; D012833:Siloxanes / Q000737:chemistry*
D015335:Molecular Probes; D012833:Siloxanes; D002338:Carotenoids