Molecular characterization of a novel RhoGAP, RRC-1 of the nematode Caenorhabditis elegans.
The GTPase-activating proteins for Rho family GTPases (RhoGAP) transduce diverse intracellular signals by negatively regulating Rho family GTPase-mediated pathways. In this study, we have cloned and characterized a novel RhoGAP for Rac1 and Cdc42, termed RRC-1, from Caenorhabditis elegans. RRC-1 was highly homologous to mammalian p250GAP and promoted GTP hydrolysis of Rac1 and Cdc42 in cells. The rrc-1 mRNA was expressed in all life stages. Using an RRC-1::GFP fusion protein, we found that RRC-1 was localized to the coelomocytes, excretory cell, GLR cells, and uterine-seam cell in adult worms. These data contribute toward understanding the roles of Rho family GTPases in C. elegans.
Delawary
Mina
M
Division of Oncology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
Nakazawa
Takanobu
T
Tezuka
Tohru
T
Sawa
Mariko
M
Iino
Yuichi
Y
Takenawa
Tadaomi
T
Yamamoto
Tadashi
T
D000595:Amino Acid Sequence; D000818:Animals; D017173:Caenorhabditis elegans / Q000737:chemistry* / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry* / Q000378:metabolism*; D020690:GTPase-Activating Proteins / Q000737:chemistry* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D014018:Tissue Distribution
D029742:Caenorhabditis elegans Proteins; D020690:GTPase-Activating Proteins; C520042:RRC-1 protein, C elegans; C085379:rho GTPase-activating protein