Characterization of photosynthetically active duckweed (Wolffia australiana) in vitro culture by Respiration Activity Monitoring System (RAMOS).
The feasibility of oxygen transfer rate (OTR) measurement to non-destructively monitor plant propagation and vitality of photosynthetically active plant in vitro culture of duckweed (Wolffia australiana, Lemnaceae) was tested using Respiration Activity Monitoring System (RAMOS). As a result, OTR proofed to be a sensitive indicator for plant vitality. The culture characterization under day/night light conditions, however, revealed a complex interaction between oxygen production and consumption, rendering OTR measurement an unsuitable tool to track plant propagation. However, RAMOS was found to be a useful tool in preliminary studies for process development of photosynthetically active plant in vitro cultures.
Rechmann
Henrik
H
Division Juelich, Universtity of Applied Science Aachen, Ginsterweg 1, Juelich, Germany.
Friedrich
Andrea
A
Forouzan
Dara
D
Barth
Stefan
S
Schnabl
Heide
H
Biselli
Manfred
M
Boehm
Robert
R
D029064:Araceae / Q000254:growth & development* / Q000378:metabolism* / Q000528:radiation effects; D008027:Light; D010100:Oxygen / Q000378:metabolism; D010101:Oxygen Consumption; D046509:Tissue Culture Techniques / Q000295:instrumentation* / Q000379:methods
D010100:Oxygen