title: Analysis of stress responsive genes induced by single-walled carbon nanotubes in BJ Foreskin cells.
abstract: Nanotechnology is finding its use as a potential technology in consumer products, defense, electronics, and medical applications by exploiting the properties of nanomaterials. Single-walled carbon nanotubes are novel forms of these nanomaterials with potential for large applications. However, the toxicity studies on this material are not explored in detail and therefore limiting its use. It has been earlier reported that single-walled carbon nanotubes induces oxidative stress and also dictates activation of specific signaling pathway in keratinocytes. The present study explores the effect of single-walled carbon nanotubes on stress genes in human BJ Foreskin cells. The results show induction of oxidative stress in BJ Foreskin cells by single-walled carbon nanotubes and increase in stress responsive genes. The genes included inducible genes like HMOX1, HMOX2, and Cyp1B1. In addition we validated increase for four genes by SWCNT, namely ATM, CCNC, DNAJB4, and GADD45A by RT-PCR. Moreover results of the altered stress related genes have been discussed and that partially explains some of the toxic responses induced by single-walled carbon nanotubes.
authors: Molecular Neurotoxicology Laboratory/Proteomics Core, Department of Biology, Texas Southern University, Houston, TX 77004, USA.
authors: Shubhashish
authors: S
authors: Sarkar
authors: Chidananda
authors: C
authors: Sharma
authors: Rajeshwari
authors: R
authors: Yog
authors: Adaikkappan
authors: A
authors: Periakaruppan
authors: Olufisayo
authors: O
authors: Jejelowo
authors: Renard
authors: R
authors: Thomas
authors: Enrique V
authors: EV
authors: Barrera
authors: Allison C
authors: AC
authors: Rice-Ficht
authors: Bobby L
authors: BL
authors: Wilson
authors: Govindarajan T
authors: GT
authors: Ramesh
mesh_terms: D002460:Cell Line; D004126:Dimethylformamide / Q000737:chemistry; D052816:Foreskin / Q000166:cytology*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects*; D007700:Kinetics; D008297:Male; D036103:Nanotechnology / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012997:Solvents / Q000737:chemistry
chemical_list: D037742:Nanotubes, Carbon; D017382:Reactive Oxygen Species; D012997:Solvents; D004126:Dimethylformamide