Antioxidant gene expression and function in in vitro-developing Schistosoma mansoni mother sporocysts: possible role in self-protection.
The ability of the larval forms of Schistosoma mansoni to invade and parasitize their molluscan host, Biomphalaria glabrata, is determined by a multitude of factors. In this study we sought to elucidate the possible mechanisms by which the invading larvae are able to counteract the potentially harmful oxidative environment presented by the host upon initial miracidial infection. This was attempted by examining the gene expression profile of parasite antioxidant enzymes of the linked glutathione-(GSH) thioredoxin (Trx) redox pathway during early intramolluscan larval development. Three such enzymes, the peroxiredoxins (Prx1, Prx2 and Prx3) were examined as to their activity and sites of expression within S. mansoni miracidia and in vitro-cultured mother sporocysts. Results of these studies demonstrated that the H(2)O(2)-reducing enzymes Prx1 and 2 are upregulated during early mother sporocyst development compared to miracidia. Immunolocalization studies further indicated that Prx1 and Prx2 proteins are expressed within the apical papillae of miracidia and tegumental syncytium of sporocysts, and are released with parasite excretory-secretory proteins (ESP) during in vitro larval transformation. Removal of Prx1 and Prx2 from larval ESP by immunoabsorption significantly reduced the ability of ESP to breakdown exogenous H(2)O(2), thereby directly linking ESP Prx proteins with H(2)O(2)-scavenging activity. Moreover, exposure of live sporocysts to exogenous H(2)O(2)stimulated an upregulation of Prx1 and 2 gene expression suggesting the involvement of H(2)O(2)-responsive elements in regulating larval Prx gene expression. These data provide evidence that Prx1 and Prx2 may function in the protection of S. mansoni sporocysts during the early stages of infection.
Vermeire
J J
JJ
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 2115 Observatory Drive, Madison, WI 53706, USA. jon.vermeire@yale.edu
Yoshino
T P
TP
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D017238:Genes, Helminth / Q000235:genetics; D006861:Hydrogen Peroxide / Q000378:metabolism; D007814:Larva / Q000737:chemistry / Q000502:physiology; D033661:Oocysts / Q000254:growth & development / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology; D010088:Oxidoreductases / Q000032:analysis / Q000235:genetics; D012550:Schistosoma mansoni / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D013879:Thioredoxins / Q000235:genetics; D013997:Time Factors
D000975:Antioxidants; D013879:Thioredoxins; D006861:Hydrogen Peroxide; D010088:Oxidoreductases