title: Effects of aestivation and starvation on the neutral lipid and phospholipid content of Biomphalaria glabrata infected with Schistosoma mansoni.
abstract: The effects of aestivation or starvation on the neutral lipid and phospholipid content of Biomphalaria glabrata patently infected with Schistosoma mansoni were determined by high-performance thin-layer chromatography-densitometry. Infected-aestivated snails were maintained in a moist chamber at 24 +/- 1 C and a relative humidity of 98 +/- 1%. Infected-starved snails were maintained in artificial spring water (ASW) at 23 +/- 1 C without exogenous food. Infected snails (the controls) were maintained in ASW at 23 +/- 1 C and fed lettuce ad libitum. The 3 groups were maintained in the laboratory for 7 days, and then the lipids from the digestive gland-gonad complex (DGG) were extracted and analyzed by class. Infected-aestivated snails exhibited greater mortality rate and weight loss after 7 days than did the infected-starved snails. The steryl ester concentration in the infected-starved snails was significantly increased (P = 0.010) compared with the controls but not compared with infected-aestivated snails; the concentration of phosphatidylcholine in infected-aestivated snails was significantly decreased (P = 0.007) compared with the controls but not when compared with the infected-starved snails. Aestivation or starvation had a significant effect on the concentration of certain lipid classes in the DGG of B. glabrata infected with S. mansoni.
authors: Department of Chemistry, Lafayette College, Easton, Pennsylvania 18042, USA.
authors: Meredith M
authors: MM
authors: White
authors: Bernard
authors: B
authors: Fried
authors: Joseph
authors: J
authors: Sherma
mesh_terms: D000818:Animals; D001700:Biomphalaria / Q000737:chemistry / Q000469:parasitology* / Q000502:physiology; D002851:Chromatography, High Pressure Liquid; D003720:Densitometry; D004952:Esters; D004956:Estivation / Q000502:physiology*; D005508:Food Deprivation / Q000502:physiology*; D008055:Lipids / Q000032:analysis*; D010713:Phosphatidylcholines / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D012550:Schistosoma mansoni / Q000502:physiology*; D013261:Sterols / Q000032:analysis
chemical_list: D004952:Esters; D008055:Lipids; D010713:Phosphatidylcholines; D010743:Phospholipids; D013261:Sterols