[Determination of bufothionine in skin of Bufo bufo gargarizans and Huachansu injection].
OBJECTIVE
To establish a HPLC method for bufothionine in the skin of Bufo bufo gargarizans and Huachansu injection.


METHODS
The samples were separated using a Lichrosob C18 column with CH3CN-H2O (10:90) as mobile phase. Flow rate was at 1.0 mL x min (-1) and the detection wavelength was at 225 nm.


RESULTS
The calibration curve of bufothionine was linear over the range of 0.0772-0.4632 microg and the average recovery was 99. 2%. The contents of bufothionine were fluctuated from 36.4-641.8 microg x g(-1) in the skin of Bufo bufo gargarizans and 22.47-33.16 microg x mL(-1) in Huachansu injection, respectively.


CONCLUSIONS
The contents of bufothionine were greatly different between cultured and wild species. The method was suitable for the quality control of the skin of Bufo bufo gargarizans and its preparation.
Dai
Li-ping
LP
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Wang
Zhi-min
ZM
Gao
Hui-min
HM
Jiang
Xu
X
Ding
Guang-zhi
GZ
D000818:Animals; D002023:Bufo bufo; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D007267:Injections; D008419:Materia Medica / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D010640:Phenothiazines / Q000032:analysis*; D011786:Quality Control; D015203:Reproducibility of Results; D012867:Skin / Q000737:chemistry*
D008419:Materia Medica; D010640:Phenothiazines; C009469:thionine