title: Application of oligonucleotide as a template for the assembly of nucleoamphiphile bearing azobenzene at the air-water interface.
abstract: In order to assemble amphiphilic adenine having azobenzene, C12AzoC5Ade (AzoAde), at the air-water interface based on the molecular recognition of DNA, we prepared aqueous linear oligothymidylic acids, dTn (n = 4, 6, 8, 10, 30), subphase as templates. Surface pressure-area (pi-A) isotherms and UV-Vis reflection absorption spectra of AzoAde were measured to investigate the effect of chain length of the oligothymidylic acid on the molecular recognition by forming a complementary A-T base pair. It was showed that AzoAde did not form a stable monolayer on the dT4 subphase and remained monomeric state. While AzoAde provided expanded monolayers and formed J-aggregates of azobenzene moieties on the dTn (n > 4) subphases even at the low molecular density. We also investigated the molecular recognition of template oligonucleotides by comparing dT30 with dA30, indicating that AzoAde had not a specific interaction with dA30 at the air-water interface due to base mismatching. The AzoAde monolayer on the dA30 subphase gave H-aggregate from monomeric state by compressing it. On the other hand, it remained J-aggregated state on dT30 subphase regardless of compression. It was, therefore, suggested that the linear oligothymidylic acids, dT, (n > 4), acted as templates for assembling AzoAde at the air-water interface.
authors: Graduate School of Science, Hokkaido University, N21W10, Kita-ku, Sapporo, Hokkaido 001-0021, Japan.
authors: Osamu
authors: O
authors: Haruta
authors: Kuniharu
authors: K
authors: Ijiro
mesh_terms: D000225:Adenine / Q000737:chemistry; D000388:Air; D001391:Azo Compounds / Q000737:chemistry*; D020029:Base Pairing; D036103:Nanotechnology; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet; D013501:Surface-Active Agents / Q000138:chemical synthesis* / Q000737:chemistry; D013938:Thymidine Monophosphate / Q000737:chemistry; D014867:Water
chemical_list: D001391:Azo Compounds; D009838:Oligodeoxyribonucleotides; D013501:Surface-Active Agents; D014867:Water; D013938:Thymidine Monophosphate; C009850:azobenzene; D000225:Adenine