title: Urethral stromal tumor with pacemaker cell phenotype.
abstract: Penile malignancies are rare in developed countries. The authors present a case of a penile urethral mesenchymal tumor occurring in a 51-year-old Caucasian male and displaying light microscopic, immunohistochemical, and ultrastructural features suggestive of a pacemaker cell type, combined with a lack of diagnostic features of any other established tumor category. The immunohistochemical profile was intensely positive for vimentin, PKC theta, and NSE and weakly positive to nonreactive for CD34 and smooth muscle actin, and entirely negative for CD117 (c-kit), S-100, and other markers. C-kit and PDGFRA gene analysis showed no mutations. Electron microscopy revealed tumor cells with plentiful cytoplasm and cytoplasmic processes/filopodia, both filled with intermediate filaments and occasional solitary focal densities. There were also prominent smooth endoplasmic reticulum cisternae, caveolae, neurosecretory granules, particularly concentrated in cytoplasmic processes, and synaptic-type structures. Poorly formed basal lamina, gap junctions, and intercellular collagen aggregates, consistent with skeinoid-type fibers, were also noted. Interstitial cells with potential pacemaker function have been recently described in the lower urinary tract, including the urethra, and this tumor may be related to this cellular phenotype.
authors: Department of Pathology, University of Maryland School of Medicine, Baltimore, Maryland, USA.
authors: William A
authors: WA
authors: Kanner
authors: Cinthia B
authors: CB
authors: Drachenberg
authors: John C
authors: JC
authors: Papadimitriou
authors: Suna
authors: S
authors: Wang
authors: Stephen J
authors: SJ
authors: Meltzer
authors: Geoffrey N
authors: GN
authors: Sklar
mesh_terms: D000199:Actins / Q000032:analysis; D018952:Antigens, CD34 / Q000032:analysis; D004252:DNA Mutational Analysis; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000032:analysis; D007984:Leydig Cell Tumor / Q000737:chemistry / Q000235:genetics / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D010641:Phenotype; D011493:Protein Kinase C / Q000032:analysis; D000074121:Protein Kinase C-theta; D017154:Stromal Cells / Q000648:ultrastructure; D013736:Testicular Neoplasms / Q000737:chemistry / Q000235:genetics / Q000648:ultrastructure*; D014523:Urethral Neoplasms / Q000737:chemistry / Q000235:genetics / Q000648:ultrastructure*; D014746:Vimentin / Q000032:analysis
chemical_list: D000199:Actins; D018952:Antigens, CD34; D007527:Isoenzymes; D014746:Vimentin; C076233:PRKCQ protein, human; D011493:Protein Kinase C; D000074121:Protein Kinase C-theta