title: Macrophagic myofasciitis in childhood: the role of scanning electron microscopy/energy-dispersive spectroscopy for diagnosis.
abstract: Macrophagic myofasciitis (MMF) is an inflammatory myopathy related to aluminum-containing vaccines. Described in 1998, most cases were reported in adults, with only 22 cases being reported in children. Three children aged between 13 months and 3(1/2) years were investigated in our institution for neuromuscular symptoms. They underwent thorough clinical, familial, and laboratory investigations, electroneuromyography, muscle biopsy with transmission electron microscopy, scanning electron microscopy/energy dispersive spectroscopy (SEM/EDS), and, in one case, brain magnetic resonance imaging. They had received regular immunizations. Two patients were hypotonic and one presented with myotonia. Muscle biopsy of all patients presented macrophagic infiltrates with intracytoplasmic aluminum content as revealed by SEM/EDS analysis. Their diverse clinical picture does not support a direct relationship between local morphologic findings and systemic symptoms. The atypical clinical presentation of these children may not result from the superposition of MMF upon a background systemic neuromyopathy, suggesting instead that they are two coincident and independent conditions. Although the finding of macrophage infiltrates in muscle tissue is not new, the identification of aluminum content is recent. The use of tissue sections for aluminum detection and mapping by SEM/EDS is conclusive for, diagnosis; it has not been reported previously in a pathology journal, to the authors' knowledge.
authors: Surgical Pathology and Electron Microscopy Laboratories, Sarah Network of Rehabilitation Hospitals, Brasilia, Brazil. kalil@sarah.br
authors: Ricardo K
authors: RK
authors: Kalil
authors: Altamir
authors: A
authors: Monteiro
authors: Maria Isabel
authors: MI
authors: Lima
authors: Eni B
authors: EB
authors: Silveira
authors: Francineide S S
authors: FS
authors: Foltran
authors: Carlos E Speck
authors: CE
authors: Martins
authors: Isabela M
authors: IM
authors: Rizzo
mesh_terms: D000276:Adjuvants, Immunologic / Q000009:adverse effects; D000535:Aluminum / Q000032:analysis; D017607:Aluminum Compounds / Q000009:adverse effects; D002675:Child, Preschool; D003593:Cytoplasm / Q000737:chemistry; D004577:Electron Probe Microanalysis; D005208:Fasciitis / Q000139:chemically induced / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D007223:Infant; D008264:Macrophages / Q000737:chemistry / Q000648:ultrastructure*; D008297:Male; D008855:Microscopy, Electron, Scanning; D018482:Muscle, Skeletal / Q000648:ultrastructure*; D009220:Myositis / Q000139:chemically induced / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D014612:Vaccines / Q000009:adverse effects
chemical_list: D000276:Adjuvants, Immunologic; D017607:Aluminum Compounds; D014612:Vaccines; D000535:Aluminum