title: Crushed bone grafts and a collagen membrane are not suitable for enhancing cartilage quality in the regeneration of osteochondral defects--an in vivo study in sheep.
abstract: Hyaline joint cartilage has only a limited potential for self-repair. Some of the published techniques for osteochondral defect therapy try to improve that potential. In this study, it was hypothesised that one of those surgical techniques, the crushed transplanted bone graft together with a collagen membrane, accelerates significantly the reconstruction of the subchondral bone plate and improves the mechanical and histological quality of repaired cartilage in osteochondral defects compared to an empty control defect. In order to test this hypothesis, defects were created in the left knee of 12 sheep and filled either with autologous crushed bone graft or left empty. The animals were sacrificed after 3 (n = 6) and 6 (n = 6) months. No differences were found either macroscopically or histomorphometrically between the bone graft and empty control defects. The biomechanical as well as the histological results of the bone graft defects were inferior to the control defects with inflammatory processes caused either by bone graft or membrane remnants. Based on the results in this sheep model, the filling of subchondral bone defects with compacted cancellous bone should be carefully reconsidered.
authors: Center for Musculoskeletal Surgery, Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, D-13353 Berlin, Germany.
authors: Hanna
authors: H
authors: Schell
authors: Jasmin
authors: J
authors: Lienau
authors: Ralf U
authors: RU
authors: Kleemann
authors: Karin
authors: K
authors: Schlichting
authors: William R
authors: WR
authors: Taylor
authors: Andreas
authors: A
authors: Weiler
authors: Georg N
authors: GN
authors: Duda
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D001847:Bone Diseases / Q000601:surgery; D016025:Bone Transplantation / Q000379:methods*; D003094:Collagen / Q000627:therapeutic use*; D005260:Female; D051457:Hyaline Cartilage / Q000254:growth & development / Q000473:pathology* / Q000601:surgery; D012038:Regeneration; D012756:Sheep
chemical_list: D003094:Collagen