title: The role of hepatocyte growth factor in the differentiation of dendritic cells from peripheral blood monocytes.
abstract: OBJECTIVE
  To find out the effects of hepatocyte growth factor (HGF) in the development of dendritic cells (DC) from the peripheral monocytes.
  
  
  METHODS
  The study was carried out in Black Sea Technical University Hospital, Trabzon, Turkey between 2003-2004. Seven different cytokine combinations were employed to assess phenotypical and functional differences of DCs from the peripheral monocytes in serum free culture media. Peripheral monocytes were incubated in media with cytokines for 5 days. The tumor necrosis factor-alpha (TNF-alpha) was added to the cell culture on day 5 and incubated for another 2 days. Surface and co-stimulating molecules on the cells were assessed by flowcytometry. The functional capacity of the DCs was evaluated on day 7 by purified protein derivative loading and subsequent lymphoproliferation test using methyl tetrazolium staining.
  
  
  RESULTS
  On the 5th day of incubation DC development was observed in all cytokine groups, but cells were superior in cultures maintained in the presence of interleukin-4 combinations with granulocyte-macrophage colony stimulating factor (GM-CSF) or with GM-CSF+HGF. Moreover, the expression of surface and co-stimulating molecules increased significantly after incubation with TNF-alpha. The effect of PPD loaded-DCs on proliferation of lymphocytes was more striking in HGF containing groups.
  
  
  CONCLUSIONS
  It was concluded that HGF supplemented cultures exert some additive effects in relation to function of monocyte-derived DCs. But HGF alone does not seem to augment monocyte-derived DC proliferation and maturation significantly.
authors: Department of Hematology, Karadeniz Technical University, School of Medicine, Trabzon 61080, Turkey. mesonmez@yahoo.com
authors: Mehmet
authors: M
authors: Sonmez
authors: Ercument
authors: E
authors: Ovali
authors: Tamer
authors: T
authors: Dikmen
authors: Mustafa
authors: M
authors: Yilmaz
authors: Murat
authors: M
authors: Erturk
authors: Bircan
authors: B
authors: Sonmez
authors: Serdar B
authors: SB
authors: Omay
mesh_terms: D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology*; D017228:Hepatocyte Growth Factor / Q000502:physiology*; D006801:Humans; D009000:Monocytes / Q000166:cytology*
chemical_list: D017228:Hepatocyte Growth Factor