Characterisation of peacock (Pavo cristatus) mitochondrial 12S rRNA sequence and its use in differentiation from closely related poultry species.
1. Poaching of peacocks, the national bird of India, is illegal. People kill this beautiful pheasant bird for tail feathers and mix the meat with chicken or turkey. Differentiation of the meat of these species is essential in order to address the ambiguity about the origin of the sample. 2. The present study was carried out to investigate the use of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of mitochondrial 12S rRNA gene for identification of these species. 3. Peacock mitochondrial 12S rRNA partial gene was amplified using universal primers, cloned and characterised. It was found to be 446 nucleotides long. 4. Sequence analysis revealed 86.8 and 84.1% similarity with reported turkey and chicken sequences, respectively. Sequence and phylogenetic analysis showed that the peacock is much closer to the turkey than the chicken. 5. PCR-RFLP of 446 bp amplicon using commonly available restriction enzymes AluI and Sau3AI produced a differential pattern for identifying these poultry species unambiguously.
Saini
M
M
Centre for Wildlife, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar - 243 122, Uttar Pradesh, India. mohini@ivri.up.nic.in
Das
D K
DK
Dhara
A
A
Swarup
D
D
Yadav
M P
MP
Gupta
P K
PK
D000818:Animals; D001483:Base Sequence; D046094:Galliformes / Q000145:classification* / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012313:RNA / Q000737:chemistry*; D000077278:RNA, Mitochondrial; D012335:RNA, Ribosomal / Q000737:chemistry*; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA
D000077278:RNA, Mitochondrial; D012335:RNA, Ribosomal; C075526:RNA, ribosomal, 12S; D012313:RNA