Adhesion of façade coating colonisers, as mediated by physico-chemical properties.
The adhesion of Klebsormidium flaccidum, Stichococcus bacillaris and Chlorella cf. mirabilis, three strains of green microalgae isolated from biofilms on façade coatings were investigated in a parallel plate flow chamber. The model surfaces tested were glass slides, and -CH(3) (mediated by octadecyltrichlorosilane [OTS] and hexamethyldisilazane [HMDZ] modification) and -NH(2) (aminopropyltriethoxysilane [APS] modification) terminated self-assembled monolayers. Algal physicochemical properties were evaluated by the microbial adhesion to solvents (MATS) assay and by contact angle measurements. The model surfaces were characterised by X-ray photoelectron spectroscopy analysis and by contact angle measurements. Predicted adhesion trends were then compared to in vitro measurements. The adhesion strength of the three algal strains followed the trend: APS > OTS > HMDZ > glass. The adhesion process thus seemed to be mediated by hydrophobic and electrostatic interactions, and was shown to be influenced by the algal culture age and the initial contact time.
Barberousse
Hélène
H
Département Enveloppe et Revêtements, Centre Scientifique et Technique du Bâtiment (CSTB), Marne-la-Vallée Cedex 2, France. helene_barberousse@yahoo.fr
Brayner
Roberta
R
Do Rego
Ana Maria Botelho
AM
Castaing
Jean-Christophe
JC
Beurdeley-Saudou
Patricia
P
Colombet
Jean-François
JF
D018441:Biofilms / Q000254:growth & development*; D002448:Cell Adhesion; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002708:Chlorella / Q000302:isolation & purification / Q000502:physiology; D000460:Chlorophyta / Q000302:isolation & purification / Q000502:physiology*; D003252:Construction Materials / Q000382:microbiology; D005602:France; D057927:Hydrophobic and Hydrophilic Interactions; D012212:Rheology; D055672:Static Electricity; D013499:Surface Properties; D013816:Thermodynamics