[Protective action of effective components of Huanglian Jiedu decoction on hypoxia and reoxygenation injury in cultured rat cerebral microvascular endothelial cells].
OBJECTIVE
To investigate the protective effect of geniposide, baicalin and berberine for the rat cerebral microvascular endothelial cell.


METHODS
The model of hypoxia and reoxygenation injury in rat cerebral microvascular endothelial cells in vitro was established. Both normal and model cells were treated with geniposide (1.024, 0.512, 0.256, 0.128, 0.064, 0.032, 0.016, 0.008 micromol x mL(-1)), baicalin (0.224, 0.112, 0.056, 0.028, 0.014, 0.007, 0.003 micromol x mL(-1)) and berberine (0.192, 0.096, 0.048, 0.024, 0.012, 0.006, 0.003 micromol x mL(-1)). Cell activity was measured by methyl thiazolyl tetrazolium (MTT) test.


RESULTS
After hypoxia/hypoglycemia cultures for 4 hour and reoxygenation for 12 hour, geniposide (0.128, 0.064, 0.032 micromol x mL(-1)), baicalin (0.028, 0.014, 0.007 micromol x mL(-1)) and berberine (0.024, 0.012, 0.006 micromol x microL(-1) could protect the injuried cerebral microvascular endothelial cells.


CONCLUSIONS
Appropriate concentration of geniposide, baicalin and berberine, which are effective components of Huanglian Jiedu decoction, could protect the injuried cerebral microvascular endothelial cells.
Yuan
Zheng-zhong
ZZ
First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China.
Zhu
Ling-qun
LQ
Pang
He
H
Shan
Ze-song
ZS
Wang
Shuo-ren
SR
Gao
Yong-hong
YH
Niu
Fu-ling
FL
D000818:Animals; D001599:Berberine / Q000302:isolation & purification / Q000494:pharmacology*; D015687:Cell Hypoxia; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000098:blood supply; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects*; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D039823:Iridoids / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology; D010100:Oxygen / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry; D011714:Pyrans / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D039823:Iridoids; D018696:Neuroprotective Agents; D011714:Pyrans; C045607:huang-lien-chieh-tu-tang; D001599:Berberine; C007835:geniposide; C038044:baicalin; D010100:Oxygen