Dialyzable uremic solutes contribute to enhanced oxidation of serum albumin in regular hemodialysis patients.
BACKGROUND
Oxidative stress (OS) is reportedly enhanced in patients receiving regular hemodialysis (HD). However, the in vivo redox state of HD patients, particularly after HD sessions, remains unclear. This study aimed to clarify the influence of HD on OS using the albumin redox state as a marker.


METHODS
Blood samples of 8 regular HD patients were obtained during the course of study. The redox state of albumin was determined using high-performance liquid chromatography.


RESULTS
The mean fraction of reversibly oxidized albumin [f(HNA-1)] declined significantly over the course of the session and reached a minimum 4 h after the session had ended (pre-HD, 36.16 +/- 7.50%; 4 h after HD, 25.71 +/- 6.41%), then gradually rose to predialytic levels. The proportion of irreversibly oxidized albumin did not change significantly over time. Positive correlations were demonstrated between f(HNA-1) and uremic small solutes in each case.


CONCLUSIONS
Accumulation of dialyzable uremic solutes may contribute to OS in HD patients.
Terawaki
Hiroyuki
H
Research Division of Dialysis and Chronic Kidney Disease, Tohoku University Graduate School of Medicine, Sendai, Japan. hterawaki@mail.tains.tohoku.ac.jp
Nakayama
Keisuke
K
Matsuyama
Yukie
Y
Nakayama
Masaaki
M
Sato
Toshinobu
T
Hosoya
Tatsuo
T
Era
Seiichi
S
Ito
Sadayoshi
S
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001806:Blood Urea Nitrogen; D002851:Chromatography, High Pressure Liquid; D003404:Creatinine / Q000097:blood; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood / Q000150:complications / Q000628:therapy; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D006435:Renal Dialysis; D012709:Serum Albumin / Q000378:metabolism*; D012995:Solubility; D014511:Uremia / Q000097:blood* / Q000209:etiology / Q000628:therapy; D014527:Uric Acid / Q000097:blood
D012709:Serum Albumin; C001702:mercaptoalbumin; D014527:Uric Acid; D003404:Creatinine