Storage of equine red blood cells as a concentrate.
The study was undertaken to determine how equine red blood cells (RBCs) survive in storage bags designed for use with human RBCs. Separated RBCs were stored in a routine manner for 35 days and examined every 7 days for storage lesions. Measured parameters included haematology, haemolysis, pH, potassium, lactate, adenosine triphosphate (ATP) and 2,3-diphosphoglycerate (2,3-DPG). All tests were performed in vitro. Haematology did not change significantly. Haemolysis increased during storage but did not exceed human limits. pH and 2,3-DPG decreased, while lactate, potassium and ATP increased. RBCs deteriorated somewhat during storage, but when compared with human in vitro parameters, remained suitable for transfusion. It is concluded that equine erythrocytes can be stored for at least 35 days before transfusion.
Niinistö
Kati
K
Department of Equine and Small Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 57, 00014 Helsinki, Finland. kati.niinisto@fimnet.fi
Raekallio
Marja
M
Sankari
Satu
S
D019794:2,3-Diphosphoglycerate / Q000097:blood; D000255:Adenosine Triphosphate / Q000097:blood; D000818:Animals; D001793:Blood Preservation / Q000379:methods / Q000662:veterinary*; D004912:Erythrocytes / Q000737:chemistry*; D006461:Hemolysis; D006736:Horses / Q000097:blood*; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000097:blood; D011188:Potassium / Q000097:blood
D019794:2,3-Diphosphoglycerate; D019344:Lactic Acid; D000255:Adenosine Triphosphate; D011188:Potassium