Increased single nucleotide discrimination in arrayed primer elongation by 4'C-modified primer probes.
Herein we describe the beneficial impact of immobilized 4'C-modified primer probes on detecting single nucleotide variations in arrayed primer extension by a DNA polymerase.
Gaster
Jens
J
Universität Konstanz, Fachbereich Chemie, Universitätsstr. 10, 78457 Konstanz, Germany.
Rangam
Gopinath
G
Marx
Andreas
A
D000483:Alleles; D002244:Carbon / Q000737:chemistry*; D017931:DNA Primers / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D015394:Molecular Structure; D009711:Nucleotides / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis
D017931:DNA Primers; D009711:Nucleotides; D002244:Carbon