title: [Establishment of urinary proteomic map on two-dimensional polyacrylamide gel electrophoresis in children with idiopathic nephrotic syndrome].
abstract: OBJECTIVE
  To establish a urinary proteomic map on two-dimensional polyacrylamide gelelectrophoresis (2-DE) in children with idiopathic nephrotic syndrome (INS).
  
  
  METHODS
  The proteins from INS children were purified by four various means, separated by 2-DEand stained by silver.
  
  
  RESULTS
  The sequential preparation of urinary proteins by acetone precipitation and dislysis, when the sample was 300 microg, resulted in a clear background, well-resolved and reproducible 2-DE urinary protemic map in children with INS.
  
  
  CONCLUSIONS
  A steady 2-DE technique for urinary protemic map in children with INS was established, which can be effectively applied in urinary proteomics of the disease.
authors: Department of Pediatrics, Second Xiangya Hospital, Central South University, Changsha, China.
authors: Juan-Juan
authors: JJ
authors: Ding
authors: Qing-Nan
authors: QN
authors: He
authors: Pin
authors: P
authors: Zhou
authors: Lei
authors: L
authors: Zhang
authors: Zhu-Wen
authors: ZW
authors: Yi
mesh_terms: D002648:Child; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008297:Male; D009404:Nephrotic Syndrome / Q000652:urine*; D011507:Proteinuria / Q000652:urine*; D040901:Proteomics / Q000379:methods*