title: [Expression, purification, and characterization of an anti-human RBC ScFv-HIV gp160 fusion protein for hemagglutination-based rapid detection of antibodies to HIV in whole blood].
abstract: OBJECTIVE
  To construct and express anti-human RBC and HIVgp160 fusion protein for rapid detection of antibody to HIV.
  
  
  METHODS
  The gene of the anti human RBC ScFv and HIV antigen were constructed together into expression vector. The fusion protein was expressed in E. coli.
  
  
  RESULTS
  The fusion protein was proved to be able to bind both anti-RBC and HIVgp160. It could cause agglutination of human RBC when HIVgp160 was present.
  
  
  CONCLUSIONS
  The fusion protein has the potential in rapid detection of HIV.
authors: Department of Virology, The No. 302 Hospital of the People's Liberation Army, Beijing, 100039, China.
authors: Yan
authors: Y
authors: Hu
authors: Jian-yang
authors: JY
authors: Yang
authors: Lei
authors: L
authors: Zhu
authors: Jun
authors: J
authors: Hou
authors: Hong-hui
authors: HH
authors: Shen
authors: Pan-yong
authors: PY
authors: Mao
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification; D001323:Autoantibodies / Q000276:immunology / Q000302:isolation & purification; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000276:immunology*; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D015483:HIV Antibodies / Q000097:blood* / Q000276:immunology; D019302:HIV Envelope Protein gp160 / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D006679:HIV Seropositivity / Q000097:blood; D006386:Hemagglutination Tests / Q000379:methods; D006801:Humans; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D015483:HIV Antibodies; D019302:HIV Envelope Protein gp160; D011993:Recombinant Fusion Proteins