Identification of dermcidin in human gestational tissue and characterization of its proteolytic activity.
Dermcidin (DCD) is a gene for an antimicrobial peptide DCD-1 in human sweat glands. It has become evident that the gene products of DCD exhibit a wide range of biological functions. In addition to its antimicrobial function, it is reported to be a neuronal survival factor, a putative oncogene in breast cancer and a proteolysis-inducing factor (PIF) that induces skeletal muscle proteolysis to cause cancer cachexia. Here we identified DCD in human placental tissue and determined its previously uncharacterized proteolytic activity. We also show that recombinant DCD induced an invasive phenotype in a human choriocarcinoma cell line JAR in vitro. This work suggests that DCD might participate in the regulation of placental function by means of modulating the proteolytic cascades on the trophoblastic cell surface, and might be involved in the pathophysiology of pregnancy-related disorders, as well as cancer and neuronal diseases.
Lee Motoyama
Jin-Pyo
JP
Department of Obstetrics and Gynecology, Keiyu Hospital, Kanagawa, Japan. jplm@pop02.odn.ne.jp
Kim-Motoyama
Hoon
H
Kim
Phyo
P
Nakagama
Hitoshi
H
Miyagawa
Kiyoshi
K
Suzuki
Kenji
K
D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002822:Choriocarcinoma / Q000473:pathology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D010455:Peptides / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism*
D010455:Peptides; C442243:dermcidin