Total internal reflection ellipsometry: ultrahigh sensitivity for protein adsorption on metal surfaces.
Total internal reflection ellipsometry (TIRE) is used to study adsorption of human serum albumin and fibrinogen on thin gold films. TIRE shows very high sensitivity for protein monolayers adsorbed on metal surfaces when surface plasmon resonance effects are utilized. The measured data, expressed in ellipsometric angles psi and D are of several orders of magnitude larger in comparison with those from similar experiments performed with traditional ellipsometry. TIRE in spectral mode opens a new path for precise studies of organic layers adsorbed on metal surfaces, with a potential for resolving the adsorbed layer microstructure.
Poksinski
Michal
M
Department of Physics, Biology and Chemistry, Laboratory of Applied Optics, Linköping University, Linköping, Sweden. micpo@ifm.liu.se
Arwin
Hans
H
D000327:Adsorption; D015374:Biosensing Techniques; D005340:Fibrinogen / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D012709:Serum Albumin / Q000737:chemistry*; D013057:Spectrum Analysis; D020349:Surface Plasmon Resonance / Q000379:methods; D013499:Surface Properties
D012709:Serum Albumin; D006046:Gold; D005340:Fibrinogen