title: Evaluation of repeatability for the automatic estimation of endothelial cell density in donor corneas.
abstract: OBJECTIVE
  The repeatability of an automatic system for estimation of endothelial cell density (ECD) from microscopy images in donor corneas was assessed.
  
  
  METHODS
  A computer program for the automatic ECD estimation from frequency analysis was previously presented. An evaluation of its repeatability was performed on a data set containing 200 corneas by assessing the accuracy and precision of automatic versus manual values. For each cornea, 2-21 images (1536 total) at 100x for automatic ECD and one image at 200x for manual ECD were available.
  
  
  RESULTS
  Accuracy of automatic ECDs was -45 (SD 99) cells/mm(2) (-2% (4%)). Precision of repeated automatic ECDs on the same cornea was 62 (30) cells/mm(2) (3% (1%)). The algorithm was also capable of identifying all non-processable images.
  
  
  CONCLUSIONS
  The proposed automatic technique proved to be reliable for its routine use in a typical eye bank setting like the one considered here.
authors: Department of Information Engineering, University of Padova, Padova, Italy. alfredo.ruggeri@unipd.it
authors: Alfredo
authors: A
authors: Ruggeri
authors: Enrico
authors: E
authors: Grisan
authors: Jan
authors: J
authors: Schroeter
mesh_terms: D002452:Cell Count / Q000379:methods; D016039:Corneal Transplantation; D004728:Endothelium, Corneal / Q000166:cytology*; D005125:Eye Banks; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008858:Microscopy, Phase-Contrast / Q000379:methods; D015203:Reproducibility of Results; D014019:Tissue Donors