title: Separation and preparative purification of arachidonic acid from microbial lipids by urea inclusion reaction and HPLC.
abstract: Arachidonic acid (AA) was separated and purified from microbial lipids by the combined method of urea inclusion reaction and reversed-phase high performance liquid chromatography. At first, AA was concentrated from free fatty acids made from microbial lipids by a urea inclusion reaction. The optimum conditions were as follows: methanol was the suitable solvent, the ratio of free fatty acids to urea to methanol was 1:2:8 (wt/wt), and the temperature of the urea inclusion reaction was -10 degrees C. The AA content was increased from 38% to 79%, and then AA was purified on a C(18) preparative column (300 mm x 30 mm I.D., d(p)=15 microm), using methanol-water (95:5, v/v) as the mobile phase, at a flow rate of 5 mL/min. The purity of AA after two steps purification reached 99%. This result indicates that the combined method of the urea inclusion reaction and reversed-phase high performance liquid chromatography is a promising technique for purification of AA.
authors: Key Laboratory of Ion Beam Bioengineering, Institute of Plasma Physics, Chinese Academy of Sciences, Hefei, Anhui, PR China. clyuan@ipp.ac.cn
authors: Chengling
authors: C
authors: Yuan
authors: Xiangqin
authors: X
authors: Wang
authors: Zengliang
authors: Z
authors: Yu
mesh_terms: D016718:Arachidonic Acid / Q000737:chemistry / Q000302:isolation & purification*; D001419:Bacteria / Q000737:chemistry* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D008055:Lipids / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013997:Time Factors; D014508:Urea / Q000737:chemistry*
chemical_list: D008055:Lipids; D012997:Solvents; D016718:Arachidonic Acid; D014508:Urea