title: Chemical synthesis and in vitro biocompatibility tests of poly (L-lactic acid).
abstract: Polylactic acid is a polymer of great technological interest, whose excellent mechanical properties, thermal plasticity, and bioresorbability render it potentially useful for environmental applications, as a biodegradable plastic and as a biocompatible material in biomedicine. This article discusses the synthesis and characterization of poly-L-lactic acid, obtained through two synthetic routes: direct polycondensation reactions without organic solvents, and in a supercritical medium. Tin complexes were used as catalysts in both polymerization reactions. The polymers were characterized by (1)HNMR, IR, GPC, DSC, and TGA techniques. In vitro biocompatibility tests were performed with human alveolar bone osteoblasts and there were assessed cell adhesion, proliferation and viability. The poly condensation reaction proved to be an excellent synthetic route to produce PLA polymers with different molar mass. The formation of polymers from lactic acid monomer was confirmed through techniques utilized. It was observed that cell adhesion and viability was not disturbed by the presence of the polymer, although the proliferation rate was decreased when compared to control.
authors: Materials Engineering-UFRGS, Av. Bento Gonçalves 9500, Setor IV-Edif. 74-123, 91501-970 Porto Alegre, Brazil. vanuscadalosto@yahoo.com.br
authors: Vanusca Dalosto
authors: VD
authors: Jahno
authors: Gabriela Benderóvicz Mendes
authors: GB
authors: Ribeiro
authors: Luís Alberto
authors: LA
authors: dos Santos
authors: Rosane
authors: R
authors: Ligabue
authors: Sandra
authors: S
authors: Einloft
authors: Maidy Rehder Wimmers
authors: MR
authors: Ferreira
authors: Karina Fittipaldi
authors: KF
authors: Bombonato-Prado
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000494:pharmacology; D002152:Calorimetry, Differential Scanning; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002850:Chromatography, Gel; D017077:Culture Media, Conditioned; D006801:Humans; D019344:Lactic Acid / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D008422:Materials Testing / Q000379:methods*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D011091:Polyesters; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D011522:Protons; D013055:Spectrophotometry, Infrared; D013237:Stereoisomerism
chemical_list: D001672:Biocompatible Materials; D017077:Culture Media, Conditioned; D011091:Polyesters; D011108:Polymers; D011522:Protons; D019344:Lactic Acid; C033616:poly(lactide)