title: Comparative evaluation of extraction procedures and method validation for determination of carbohydrates in cereals and dairy products by capillary electrophoresis.
abstract: This work describes the development and application of two optimized electrolytes: 15 mmol/L sorbate, 0.2 mmol/L CTAB, and 35 mmol/L sodium hydroxide for the determination of fructose, glucose, maltose, maltotriose, and sucrose in cereals and 15 mmol/L sorbate, 0.3 mmol/L CTAB, and 55 mmol/L sodium hydroxide for the determination of fructose, glucose, galactose, lactose, and sucrose in dairy products. Both methods were validated with respect to linearity, limits of detection (using both signal-to-noise ratio and the Eurachem approach), recovery tests, and intra- and inter-day precision exhibiting adequate performance. Additionally, statistically similar results were obtained in a comparative study of extraction procedures for carbohydrates using the AOAC protocol, ultrasound extraction, magnetic stirring, and sample dissolution.
authors: Institute of Chemistry, University of Sao Paulo, Sao Paulo, Brazil.
authors: Alessandra V
authors: AV
authors: Jager
authors: Fernando G
authors: FG
authors: Tonin
authors: Marina F M
authors: MF
authors: Tavares
mesh_terms: D002241:Carbohydrates / Q000032:analysis*; D003611:Dairy Products / Q000032:analysis*; D002523:Edible Grain / Q000737:chemistry*; D004573:Electrolytes; D019075:Electrophoresis, Capillary / Q000379:methods*
chemical_list: D002241:Carbohydrates; D004573:Electrolytes