Peptidoglycan recognition protein (PGRP) from eri-silkworm, Samia cynthia ricini; protein purification and induction of the gene expression.
Peptidoglycan recognition protein (PGRP) was isolated from immunized hemolymph of the wild silkworm, Samia cynthia ricini, detecting the biding activity with (125)I-labeled peptidoglycan (PGN). The binding specificity of PGRP was tested by competitive inhibition of the binding to (125)I-labeled-PGN by a large excess amount of non-labeled-PGN or other glucans. The binding to labeled uncross-linked Lys-type PGN from Micrococcus luteus was strongly inhibited by non-labeled-PGN of the same structure and meso-diaminopimelic acid (DAP)-type cross-linked PGN from Bacillus cell wall, but only a little by cross-linked PGN from M. luteus cell wall. The PGRP cDNA encodes a 193 amino acid open reading frame. The deduced amino acid sequence had 62 to 91% identities to known lepidopteran PGRPs, but less than 40% to Drosophila PGRPs. The PGRP gene constitutively expressed at a low level in naive fat body, and strongly induced by an injection of DAP-type cross-linked and Lys-type uncross-linked PGNs, but only weakly by Lys-type cross-linked PGN from M. luteus. The silkworm possibly distinguish between PGNs based on the structure of cross-linking peptide, but has less if any preference for the diamino acid residue of the stem peptide.
Onoe
Hiroko
H
Department of Biochemistry and Biotechnology, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.
Matsumoto
Akiyoshi
A
Hashimoto
Kazuhiko
K
Yamano
Yoshiaki
Y
Morishima
Isao
I
D000595:Amino Acid Sequence; D000818:Animals; D001407:Bacillus / Q000737:chemistry; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D004330:Drosophila / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology*; D019476:Insect Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D016982:Micrococcus luteus / Q000737:chemistry; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics* / Q000378:metabolism*; D010457:Peptidoglycan / Q000737:chemistry; D011485:Protein Binding / Q000502:physiology; D017386:Sequence Homology, Amino Acid
D002352:Carrier Proteins; D019476:Insect Proteins; D010457:Peptidoglycan; C118897:peptidoglycan recognition protein