title: Short peptides derived from the NH2-terminus of subclass IIa bacteriocin enterocin CRL35 show antimicrobial activity.
abstract: OBJECTIVE
  Subclass IIa bacteriocins are characterized by a hydrophilic N-terminal domain that shares a YGNGVxCxxxxC consensus and a variable hydrophobic C-terminus. Enterocin CRL35 is a 43-amino-acid heat stable peptide with antilisterial activity. Short synthetic peptides derived from the N-terminal half of enterocin CRL35 and other subclass IIa bacteriocins were evaluated for antimicrobial properties.
  
  
  METHODS
  In vitro activities of synthetic peptides were evaluated in complex, chemically defined and minimal media. MIC assays were performed by the agar well-diffusion method. Fluorescence assays to evaluate the dissipation of membrane potentials in intact cells were carried out. Time-kill kinetics of Listeria innocua cells with the active peptide were performed.
  
  
  CONCLUSIONS
  A 15-mer peptide derived from enterocin CRL35 inhibited the growth of L. innocua and Listeria monocytogenes in synthetic/minimal media and dissipated the membrane potential of sensitive cells, with MICs of 10 and 50 microM, respectively. 15-mer derivatives from other class IIa bacteriocins (mesentericin Y105, pediocin PA-1 and piscicolin 126) also showed antimicrobial activities.
authors: Centro de Referencia para Lactobacilos, Chacabuco 145, SM de Tucumán, Tucumán, Argentina.
authors: Emiliano
authors: E
authors: Salvucci
authors: Lucila
authors: L
authors: Saavedra
authors: Fernando
authors: F
authors: Sesma
mesh_terms: D000900:Anti-Bacterial Agents; D001419:Bacteria / Q000187:drug effects; D001430:Bacteriocins / Q000737:chemistry* / Q000494:pharmacology*; D003470:Culture Media; D007700:Kinetics; D008087:Listeria / Q000187:drug effects; D008089:Listeria monocytogenes / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D003470:Culture Media; D010455:Peptides; C121783:enterocin CRL35