title: Localization of phosphorylated TrkA in carrier vesicles involved in its nuclear translocation in U251 cell line.
abstract: A number of transmembrane receptors are targeted to the nucleus and convincingly localized therein. However, what remains a conundrum is how these cell-surface receptors end up in the nucleus. In this study, we reported that the transmembrane receptor phosphorylated TrkA was located in a series of carrier vesicles, including ring-like vesicles near the plasma membrane, large core vesicles and small dense core vesicles around the nuclei, as well as in the nucleus in human glioma cell line U251 using immunocytochemistry and immunofluorescence staining. Meanwhile, we also showed that small dense core vesicles budded from large core vesicles, and interacted with the nuclear envelope. Accordingly, our results suggested that such a series of membrane compartments might be involved in the pathway of nuclear translocation of the transmembrane receptor TrkA.
authors: School of Medicine, Jiangsu University, Zhenjiang 212013, China. ahg5@163.com
authors: AiHua
authors: A
authors: Gong
authors: ZhiJian
authors: Z
authors: Zhang
authors: DeSheng
authors: D
authors: Xiao
authors: Yong
authors: Y
authors: Yang
authors: YongZhong
authors: Y
authors: Wang
authors: YongChang
authors: Y
authors: Chen
mesh_terms: D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000201:enzymology; D002467:Cell Nucleus / Q000201:enzymology; D005910:Glioma; D006801:Humans; D007150:Immunohistochemistry; D009685:Nuclear Envelope / Q000201:enzymology; D010766:Phosphorylation; D021381:Protein Transport; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*
chemical_list: D011505:Protein-Tyrosine Kinases