title: Selective photo-cross-linking of 2'-O-psoralen-conjugated oligonucleotide with RNAs having point mutations.
abstract: It has been reported that point mutations in genes are responsible for various cancers and the selective regulation of the gene expression is an important issue to develop a new type of anticancer drugs. In this report, we present a new type of antisense molecule that photo-cross-links to an oligoribonucleotide having a point mutation site in a sequence specific manner. 2'-O-psoralen-conjugated adenosine was synthesized in four steps from adenosine and introduced in the middle of an oligodeoxyribonucleotide (2'-Ps-oligo). Compared with 5'-O-psoralen-conjugated oligodeoxyribonucleotide (5'-Ps-oligo), which has a psoralen at the 5'-end, 2'-Ps-oligo more selectively photo-cross-linked to a pyrimidine base of the site of alteration from purine to pyrimidine in the oligoribonucleotide.
authors: Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Kyoto, Japan.
authors: Maiko
authors: M
authors: Higuchi
authors: Asako
authors: A
authors: Yamayoshi
authors: Takasei
authors: T
authors: Yamaguchi
authors: Reiko
authors: R
authors: Iwase
authors: Tetsuji
authors: T
authors: Yamaoka
authors: Akio
authors: A
authors: Kobori
authors: Akira
authors: A
authors: Murakami
mesh_terms: D002621:Chemistry / Q000379:methods; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000494:pharmacology*; D005363:Ficusin / Q000737:chemistry*; D008027:Light; D008956:Models, Chemical; D009841:Oligonucleotides / Q000737:chemistry*; D016376:Oligonucleotides, Antisense / Q000737:chemistry; D017354:Point Mutation; D012313:RNA / Q000737:chemistry* / Q000235:genetics*; D013696:Temperature; D014466:Ultraviolet Rays
chemical_list: D003432:Cross-Linking Reagents; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; D012313:RNA; D005363:Ficusin