title: Oxypurine and nucleoside concentrations in renal veins during reperfusion are predictors of early graft function.
abstract: OBJECTIVE
  To examine whether purine and pyrimidine nucleoside concentrations as well as oxypurine concentrations in renal and peripheral veins during reperfusion correlate with graft function parameters.
  
  
  METHODS
  The study population comprised 25 recipients of cadaver kidney transplant. A first blood sample was taken from the recipient's peripheral vein before anastomosing the kidney allograft vessels with the recipient's iliac vessels. Subsequent samples were taken from the allograft renal vein and the recipient's peripheral vein 5 min after beginning reperfusion. High-performance liquid chromatography was done to measure plasma concentrations of the oxypurines hypoxanthine (Hyp), xanthine (Xan) and uric acid and the nucleosides guanosine, inosine (Ino) and uridine (Urd).
  
  
  RESULTS
  Concentrations of Hyp, Xan and Ino were significantly higher in the renal than the peripheral vein. The differences between the Xan, Hyp, Ino and Urd plasma concentrations in the renal and peripheral veins before and 5 min after reperfusion correlated positively and significantly with serum creatinine concentrations 24 and 72 h after graft transplantation. Moreover, the concentrations of Hyp were significantly increased in renal transplant recipients with delayed graft function.
  
  
  CONCLUSIONS
  The results of this study suggest that the concentration of Hyp in the kidney allograft vein can be a useful predictor of early graft function.
authors: Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University, Szczecin, Poland. domanle@sci.pam.szczecin.pl
authors: Leszek
authors: L
authors: Domanski
authors: Krzysztof
authors: K
authors: Safranow
authors: Katarzyna
authors: K
authors: Jakubowska
authors: Marek
authors: M
authors: Myslak
authors: Jacek
authors: J
authors: Rozanski
authors: Marek
authors: M
authors: Ostrowski
authors: Grazyna
authors: G
authors: Dutkiewicz
authors: Andrzej
authors: A
authors: Pawlik
authors: Kazimierz
authors: K
authors: Ciechanowski
mesh_terms: D005260:Female; D006151:Guanosine / Q000097:blood*; D006801:Humans; D019271:Hypoxanthine / Q000097:blood*; D007288:Inosine / Q000097:blood*; D016030:Kidney Transplantation / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D009705:Nucleosides / Q000097:blood*; D011237:Predictive Value of Tests; D012082:Renal Veins; D015424:Reperfusion; D014527:Uric Acid / Q000097:blood*; D014529:Uridine / Q000097:blood*; D019820:Xanthine / Q000097:blood*
chemical_list: D009705:Nucleosides; D006151:Guanosine; D019820:Xanthine; D014527:Uric Acid; D019271:Hypoxanthine; D007288:Inosine; D014529:Uridine