Application and field validation of a PCR assay for the detection of Mycoplasma hyopneumoniae from swine lung tissue samples.
A PCR assay was validated for the detection of Mycoplasma hyopneumoniae in porcine lung tissue. The detection limit of the assay was 0.18 colony-forming units/g of lung sample spiked with M. hyopneumoniae. In field validation, 426 pigs from 220 cases were examined for M. hyopneumoniae infection by M. hyopneumoniae PCR and a fluorescent antibody (FA) test. In total, 103 pig lungs (24.2%) were positive in the PCR test, and 69 pig lungs (16.2%) were positive in the FA test, among which, 62 pigs were positive for both PCR and FA test. Most of the PCR-positive but FA test-negative cases had lesions compatible with M. hyopneumoniae infection. With Bayesian modeling, the diagnostic sensitivity and specificity of the PCR were determined to be 97.3% and 93.0%, respectively.
Cai
Hugh Y
HY
Animal Health Laboratory, Laboratory Services Division, University of Guelph, PO Box 3612, Guelph, Ontario, Canada N1H 6R8. hcai@lsd.uoguelph.ca
van Dreumel
Tony
T
McEwen
Beverly
B
Hornby
Geoff
G
Bell-Rogers
Patricia
P
McRaild
Pat
P
Josephson
Gaylan
G
Maxie
Grant
G
D000818:Animals; D008168:Lung / Q000382:microbiology*; D045705:Mycoplasma hyopneumoniae / Q000235:genetics / Q000302:isolation & purification*; D045729:Pneumonia of Swine, Mycoplasmal / Q000175:diagnosis* / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine