Establishment of autologous embryonic stem cells derived from preantral follicle culture and oocyte parthenogenesis.
OBJECTIVE
To evaluate whether autologous embryonic stem cells can be established without generating clone embryos.


METHODS
Prospective model study.


METHODS
Gamete and stem cell biotechnology laboratory in Seoul National University, Seoul, Korea.


METHODS
F1 hybrid B6D2F1 mice.


METHODS
Preantral follicles were cultured, and oocytes matured in the follicles were parthenogenetically activated.


METHODS
Preimplantation development and stem cell characterization.


RESULTS
More intrafollicular oocytes that were retrieved from secondary follicles matured and developed into blastocysts after parthenogenesis than those that were retrieved from primary follicles. Of those 35 blastocysts derived from 193 parthenotes, one line of colony-forming cells was established from the culturing of early secondary follicles. The established cells were positive for embryonic stem cell-specific markers and had normal diploid karyotype and telomerase activity. They differentiated into embryoid bodies in vitro and teratomas in vivo. Inducible differentiation of the established cells into neuronal lineage cells also was possible.


CONCLUSIONS
Autologous embryonic stem cells can be established by preantral follicle culture and oocyte parthenogenesis. A combined technique of follicle culture and oocyte parthenogenesis that does not use developmentally competent oocytes has the potential to replace somatic cell nuclear transfer for autologous cell therapy.
Lee
Seung Tae
ST
Department of Agricultural Biology, Gamete and Stem Cell Biotechnology Laboratory, Seoul National University, Seoul, Korea.
Choi
Mun Hwan
MH
Lee
Eun Ju
EJ
Gong
Seung Pyo
SP
Jang
Mi
M
Park
Sang Hyun
SH
Jee
Hyang
H
Kim
Dae Yong
DY
Han
Jae Yong
JY
Lim
Jeong Mook
JM
D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000502:physiology; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002460:Cell Line; D019070:Cell Lineage; D049109:Cell Proliferation; D053595:Embryonic Stem Cells / Q000502:physiology* / Q000637:transplantation; D005240:Feasibility Studies; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D009865:Oocytes / Q000502:physiology*; D006080:Ovarian Follicle / Q000166:cytology / Q000502:physiology*; D010312:Parthenogenesis; D033581:Stem Cell Transplantation; D013724:Teratoma / Q000473:pathology; D046509:Tissue Culture Techniques; D014182:Transplantation, Autologous