Immunohistochemical detection of hepatocyte nuclear factor 1beta in ovarian and endometrial clear-cell adenocarcinomas and nonneoplastic endometrium.
Recent studies have noted specific expression of hepatocyte nuclear factor (HNF) 1beta in ovarian clear-cell adenocarcinoma (CCA). In this study, we aimed to determine whether HNF-1beta can be a specific marker of CCA in both the ovary and the endometrium and to assess the pathological significance of HNF-1beta expression in CCAs. We examined HNF-1beta expression immunohistochemically in 186 ovarian carcinomas, including 40 CCAs; 33 endometrial carcinomas, including 5 CCAs; 22 endometria at different stages of the menstrual cycle (5 in the proliferative, 12 in the secretory, and 5 in the menstrual phases); and 7 gestational endometria. The incidence of HNF-1beta immunoreactivity differed significantly between CCAs and other histology in both the ovary (100% in the former versus 2% in the latter) and the endometrium (100% in the former versus 0% in the latter) (P < .0001 each). In nonneoplastic endometrium, 25% or more immunoreactive cells were confined to the mid-to-late secretory phase of the menstrual cycle and gestational endometrium. HNF-1beta would be an excellent marker for distinguishing CCAs from other lesions in both the ovary and the endometrium. HNF-1beta expression seems to be associated with physiopathological cytoplasmic glycogen accumulation in these organs.
Yamamoto
Sohei
S
Department of Basic Pathology, National Defense Medical College, Tokorozawa, Saitama 359-8513, Japan.
Tsuda
Hitoshi
H
Department of Basic Pathology, National Defense Medical College, Tokorozawa, Saitama 359-8513, Japan. Electronic address: htsuda@ndmc.ac.jp.
Aida
Shinsuke
S
Department of Laboratory Medicine, National Defense Medical College, Saitama 359-8513, Japan.
Shimazaki
Hideyuki
H
Department of Laboratory Medicine, National Defense Medical College, Saitama 359-8513, Japan.
Tamai
Seiichi
S
Department of Laboratory Medicine, National Defense Medical College, Saitama 359-8513, Japan.
Matsubara
Osamu
O
Department of Basic Pathology, National Defense Medical College, Tokorozawa, Saitama 359-8513, Japan.
D018262:Adenocarcinoma, Clear Cell / Q000378:metabolism*; D016889:Endometrial Neoplasms / Q000378:metabolism*; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D051539:Hepatocyte Nuclear Factor 1-beta / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D010051:Ovarian Neoplasms / Q000378:metabolism*
D051539:Hepatocyte Nuclear Factor 1-beta