[Comparative evaluation of Hebei HIV-1 p24 kit for the detection of human immunodeficiency virus].
OBJECTIVE
To probe into the feasibility of screening anti-HIV compounds by using HIV-1 p24 detection kit made by Hebei Medical University.


METHODS
The sensitivity, reproducibility and efficacy of the Hebei p24 kit were evaluated compared with the commercially available Vironostika HIV-1 Antigen Microelisa System (Biomerieux).


RESULTS
Hebei p24 kit had high sensitivity and good reproducibility. In vitro screening demonstrated that there was no statistically significant difference (P greater than 0.05) between these two kits in assessing anti-HIV compounds.


CONCLUSIONS
Hebei p24 kit could be used as an easily affordable alternative method for detection of HIV-1 in screening anti-HIV compounds.
Yang
Yi-shu
YS
College of Life Science and Bio-engineering, Beijing University of Technology, Beijing 100022, China.
Wang
Run-tian
RT
Zhang
Xiao-guang
XG
Zhang
Hong-zhong
HZ
Wang
Hui-fen
HF
Li
Ze-lin
ZL
Zeng
Yi
Y
D019380:Anti-HIV Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation / Q000379:methods; D005240:Feasibility Studies; D016655:HIV Core Protein p24 / Q000032:analysis*; D015497:HIV-1 / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology; D006801:Humans; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D015203:Reproducibility of Results
D019380:Anti-HIV Agents; D016655:HIV Core Protein p24; D011933:Reagent Kits, Diagnostic