title: Urinary biomarker of oxidative stress correlating with outcome in critically septic patients.
abstract: OBJECTIVE
  To determine whether urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG), an in vivo parameter of oxidative stress, is correlated with the outcome of critically septic patients.
  
  
  METHODS
  Clinical outcome study in an adult medical intensive care unit (ICU).
  
  
  METHODS
  85 consecutive septic patients (59 men, 26 women).
  
  
  RESULTS
  Patient characteristics and the clinical course were examined. Urinary 8-OHdG was analyzed using isotope-dilution liquid chromatography with tandem mass spectrometry (LC/MS/MS). ICU mortality was 25.9% (22/85) and hospital mortality 38.8% (33/85). Survivors' APACHEII scores on days 1 and 3 and the difference between them differed significantly from those of nonsurvivors (day 1, 21.0+/-7.1 vs. 25.9+/-8.0; day 3, 15.0+/-5.8 vs. 23.2+/-8.3; difference, 6.0+/-5.5 vs. 1.7+/-6.6). Urinary 8-OHdG was significantly lower in survivors than in nonsurvivors on day 1 (1.8+/-2.4 vs. 3.0+/-2.4). The area under receiver operating characteristic curve analysis for the association between day 1 urinary 8-OHdG and ICU mortality was 0.71. The comparison performed upon discharge from hospital revealed similar results.
  
  
  CONCLUSIONS
  This is a preliminary study. Excretion of urinary 8-OHdG, as measured using isotope-dilution LC/MS/MS, and the APACHE II score were correlated with the outcome of critically septic patients in medical ICU.
authors: Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, China Medical University Hospital, 2 Yuh-Der Rd., Taichung 404, Taiwan.
authors: Wei-Erh
authors: WE
authors: Cheng
authors: Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, China Medical University Hospital, 2 Yuh-Der Rd., Taichung 404, Taiwan.
authors: Chuen-Ming
authors: CM
authors: Shih
authors: Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, China Medical University Hospital, 2 Yuh-Der Rd., Taichung 404, Taiwan.
authors: Liang-Wen
authors: LW
authors: Hang
authors: Division of Environmental Health and Occupational Medicine, National Health Research Institute, 35, Keyan Road, Zhunan Town, Miaoli County 350, Taiwan.
authors: Kuen-Yuh
authors: KY
authors: Wu
authors: Department of Nutrition, China Medical University, 91 Hsueh-Shih Road, Taichung 404, Taiwan.
authors: Hsin-Ling
authors: HL
authors: Yang
authors: Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, China Medical University Hospital, 2 Yuh-Der Rd., Taichung 404, Taiwan.
authors: Wu-Huei
authors: WH
authors: Hsu
authors: Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, China Medical University Hospital, 2 Yuh-Der Rd., Taichung 404, Taiwan. derrick.hsia@msa.hinet.net.
authors: Te-Chun
authors: TC
authors: Hsia
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D018806:APACHE; D000368:Aged; D015415:Biomarkers / Q000652:urine*; D002853:Chromatography, Liquid; D016638:Critical Illness; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D005260:Female; D006801:Humans; D008297:Male; D018384:Oxidative Stress; D011865:Radioisotope Dilution Technique; D018805:Sepsis / Q000401:mortality* / Q000652:urine; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine