title: Cationic supported lipid bilayers for antigen presentation.
abstract: Polystyrene sulfate (PSS) particles (301 nm mean diameter) were covered with single cationic dioctadecyldimethylammonium bromide (DDA) bilayers and used for antigen adsorption and presentation. The antigen was a mixture of purified 18/14 Taenia crassiceps proteins (18/14-Tcra). Firstly, the DDA/PSS assembly was characterized at 1mM NaCl and 5 x 10(9) PSS particles/mL over a range of DDA concentrations (0.001-1mM) by means of dynamic light scattering for particle sizing and zeta-potential analysis. 0.01 mM DDA is enough to produce homodisperse and cationic bilayer-covered particles. Secondly, under these experimental conditions, 18/14-Tcra adsorption isotherms onto biomimetic particles or aluminium hydroxide (Al(OH)3) yield limiting adsorption of 0.36 and 1.32 mg protein/mg biomimetic particles or Al(OH)3, respectively. Finally, in mice, superior humoral and cellular immunoresponse from serum IgG and footpad swelling was obtained for antigen/biomimetic particles in comparison to conventional Al(OH)3. Cationic bilayer-covered particles are a novel, highly organized and, possibly, general immunoadjuvant for antigen presentation and subunit vaccine design.
authors: Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, CP 26077, Av. Lineu Prestes 748 - Butantã, CEP 05513-970, São Paulo, SP, Brazil.
authors: N
authors: N
authors: Lincopan
authors: N M
authors: NM
authors: Espíndola
authors: A J
authors: AJ
authors: Vaz
authors: A M
authors: AM
authors: Carmona-Ribeiro
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry; D000536:Aluminum Hydroxide / Q000737:chemistry / Q000276:immunology; D000818:Animals; D000917:Antibody Formation; D000947:Antigens, Helminth / Q000737:chemistry / Q000276:immunology*; D002412:Cations; D002626:Chemistry, Pharmaceutical; D004337:Drug Carriers; D004339:Drug Compounding; D005260:Female; D006968:Hypersensitivity, Delayed / Q000276:immunology; D008027:Light; D008051:Lipid Bilayers; D008055:Lipids / Q000737:chemistry*; D051379:Mice; D010316:Particle Size; D011137:Polystyrenes / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D012542:Scattering, Radiation; D012965:Sodium Chloride / Q000737:chemistry; D013621:Taenia / Q000276:immunology*; D014612:Vaccines / Q000737:chemistry / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000947:Antigens, Helminth; D002412:Cations; D004337:Drug Carriers; D008051:Lipid Bilayers; D008055:Lipids; D011137:Polystyrenes; D000644:Quaternary Ammonium Compounds; D014612:Vaccines; C071389:polystyrene sulfate; C015831:dimethyldioctadecylammonium; D012965:Sodium Chloride; D000536:Aluminum Hydroxide