title: Adhesion of façade coating colonisers, as mediated by physico-chemical properties.
abstract: The adhesion of Klebsormidium flaccidum, Stichococcus bacillaris and Chlorella cf. mirabilis, three strains of green microalgae isolated from biofilms on façade coatings were investigated in a parallel plate flow chamber. The model surfaces tested were glass slides, and -CH(3) (mediated by octadecyltrichlorosilane [OTS] and hexamethyldisilazane [HMDZ] modification) and -NH(2) (aminopropyltriethoxysilane [APS] modification) terminated self-assembled monolayers. Algal physicochemical properties were evaluated by the microbial adhesion to solvents (MATS) assay and by contact angle measurements. The model surfaces were characterised by X-ray photoelectron spectroscopy analysis and by contact angle measurements. Predicted adhesion trends were then compared to in vitro measurements. The adhesion strength of the three algal strains followed the trend: APS > OTS > HMDZ > glass. The adhesion process thus seemed to be mediated by hydrophobic and electrostatic interactions, and was shown to be influenced by the algal culture age and the initial contact time.
authors: Département Enveloppe et Revêtements, Centre Scientifique et Technique du Bâtiment (CSTB), Marne-la-Vallée Cedex 2, France. helene_barberousse@yahoo.fr
authors: Hélène
authors: H
authors: Barberousse
authors: Roberta
authors: R
authors: Brayner
authors: Ana Maria Botelho
authors: AM
authors: Do Rego
authors: Jean-Christophe
authors: JC
authors: Castaing
authors: Patricia
authors: P
authors: Beurdeley-Saudou
authors: Jean-François
authors: JF
authors: Colombet
mesh_terms: D018441:Biofilms / Q000254:growth & development*; D002448:Cell Adhesion; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002708:Chlorella / Q000302:isolation & purification / Q000502:physiology; D000460:Chlorophyta / Q000302:isolation & purification / Q000502:physiology*; D003252:Construction Materials / Q000382:microbiology; D005602:France; D057927:Hydrophobic and Hydrophilic Interactions; D012212:Rheology; D055672:Static Electricity; D013499:Surface Properties; D013816:Thermodynamics