title: Effect of astragaloside on cardiomyocyte apoptosis in murine coxsackievirus B3 myocarditis.
abstract: Astragaloside is the major component of Astragalus membranaceus, one of the Chinese medical herbs, and has several pharmacological actions on cardiovascular system, including positive inotropic, anti-arrhythmia and anti-oxidant activities. We have investigated the effect of astragaloside on cardiomyocyte apoptosis and expression of apoptosis-associated genes in mice with coxsackievirus B(3) (CVB(3))-induced myocarditis, the former of which has been detected by terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay and the latter determined by using a focused microarray. Results showed that cardiomyocyte apoptosis and expression of various apoptosis associated genes are inhibited after treatment with astragaloside. The anti-apoptotic activity of astragaloside may contribute to the improvement of clinical outcomes in treating myocarditis with pharmaceutics of Astragalus membranaceus.
authors: Zhejiang Hospital, 12 Ling Yin Road, Hangzhou, Zhejiang Province 310013, China
authors: Z-C
authors: ZC
authors: Zhang
authors: S-J
authors: SJ
authors: Li
authors: Y-Z
authors: YZ
authors: Yang
authors: R-Z
authors: RZ
authors: Chen
authors: J-B
authors: JB
authors: Ge
authors: H-Z
authors: HZ
authors: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D003384:Coxsackievirus Infections / Q000188:drug therapy*; D004770:Enterovirus / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D009205:Myocarditis / Q000188:drug therapy* / Q000821:virology; D032383:Myocytes, Cardiac / Q000187:drug effects*; D012503:Saponins / Q000494:pharmacology* / Q000627:therapeutic use; D014315:Triterpenes / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D012503:Saponins; D014315:Triterpenes; C052064:astragaloside A