Morphological and functional evaluation of angiogenesis of a xenografted human sarcoma in nude mice.
The implantation of tumour cells in normal tissues and the subsequent induction of angiogenesis by the growing xenograft were studied by means of immunohistochemistry and digital image analysis. Tumour growth was induced by injection of a human spindle cell sarcoma (ES3) into the subcutis of HsdCpb:NMRI-nu/nu mice. In vivo injection of Hoechst 33342 was used as a marker of perfusion. The vasculature was stained with specific antibodies and subsequently analysed by digital image analysis. Starting at day 3 up to day 6, angiogenesis could be detected and the relative amount of perfusion within the investigated area reached a peak at day 6. This method, which allows investigation of both functional and morphometric characteristics of human xenograft vasculature, serves as an excellent assay for evaluation of antiangiogenic therapies in translational research of experimental tumours.
Pöttgen
Christoph
C
Department of Radiotherapy, Central Animal Laboratory, University of Duisburg-Essen Medical School, Essen, Germany. christoph.poettgen@uni-essen.de
Hilken
Gero
G
Vanhoefer
Udo
U
Stuschke
Martin
M
Stüben
Georg
G
D000818:Animals; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008819:Mice, Nude; D008954:Models, Biological; D009368:Neoplasm Transplantation; D009389:Neovascularization, Pathologic / Q000378:metabolism*; D012509:Sarcoma / Q000098:blood supply*; D013997:Time Factors; D014183:Transplantation, Heterologous