title: Compositions and biological activities of essential oils of Kadsura longepedunculata and Schisandra sphenanthera.
abstract: The chemical compositions, antimicrobial activities, antioxidant activities and cytotoxicities of the essential oils isolated from the root of Kadsura longepedunculata Finet et Gagnep (KLREO) and the fruit of Schisandra sphenanthera Rehd. et Wills. (SSFEO) were investigated.The analyses of gas chromatography-mass spectrometry (GC-MS) showed that cadinane type compounds and their derivatives were rich in both oils (54.2% and 39.7%, respectively) and delta-cadinene was the major component of both oils (13.8% and 25.6%, respectively). The antimicrobial activities of both oils were evaluated against five microorganisms with the disc diffusion and the broth micro-dilution method. Results showed that Gram-positive bacteria were more sensitive to both oils than Gram-negative bacteria and the yeast. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the oil of KLREO were lower than those of SSFEO, indicating that the former possessed slightly stronger antibacterial capability than the latter. The reducing power and lipid peroxidation assays were employed to study the potential antioxidant activities of both oils. Both oils remarkably decreased the content of malondialdehyde (MDA) in rat liver homogenate in a dose dependent manner. The antioxidant activities of KLREO appeared to be more potent than that of SSFEO. The oils of KLREO and SSFEO exhibited concentration-dependent cytotoxicities and were proved to be toxic to HepG2 cells with IC(50) of 147 and 189 mug/ml, respectively.
authors: State Key Laboratory of Genetic Engineering, Fudan University, 220 Handan Road, Shanghai, 200433, China.
authors: Lei
authors: L
authors: Song
authors: Jie-Ying
authors: JY
authors: Ding
authors: Cui
authors: C
authors: Tang
authors: Chun-Hua
authors: CH
authors: Yin
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D002460:Cell Line; D003603:Cytotoxins / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005638:Fruit / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007535:Isomerases / Q000032:analysis; D032135:Kadsura / Q000737:chemistry*; D008099:Liver / Q000737:chemistry; D008315:Malondialdehyde / Q000032:analysis; D008826:Microbial Sensitivity Tests; D010938:Plant Oils / Q000737:chemistry* / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D000081223:Polycyclic Sesquiterpenes; D032141:Schisandra / Q000737:chemistry*; D012717:Sesquiterpenes / Q000032:analysis; D015003:Yeasts / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D003603:Cytotoxins; D010938:Plant Oils; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes; C508039:cadinane; D008315:Malondialdehyde; D007535:Isomerases; C097608:delta-cadinene synthase