title: High risk of temporary alteration of semen parameters after recent acute febrile illness.
abstract: OBJECTIVE
  To report parameters in semen samples and sperm deoxyribonucleic acid integrity in a fertile volunteer presenting a 2-day fever of 39 degrees -40 degrees C.
  
  
  METHODS
  Case report.
  
  
  METHODS
  University-affiliated teaching hospital.
  
  
  METHODS
  None.
  
  
  METHODS
  Semen samples from a fertile volunteer of proven fertility were obtained and analyzed before the febrile illness episode and at days 15, 37, 58, 79, and >180 after the fever.
  
  
  METHODS
  Semen parameters (total sperm count, motility a+b, and vitality), sperm protamination state, measured by sperm chromatin structure assay (SCSA) and apoptotic activities, measured by terminal uridine nick-end labeling (TUNEL) assay.
  
  
  RESULTS
  Total sperm count significantly decreased at days 15, 37, and 58 after the fever and returned to normal by day 79 after the fever. The percentage of motility significantly decreased at days 15 and 37 after the fever and returned to normal by day 58. Vitality score also showed a slight, although not statistically significant, decrease after the fever. The DNA fragmentation index (DFI, a SCSA parameter), which defines abnormal chromatin structure, significantly increased by 24% and 36% at days 15 and 37 after the fever, respectively, and decreased to 15% and 8% when reaching days 58 and 79 after the fever. High DNA stainability (HDS, a SCSA parameter) also significantly increased at day 37 after the fever. On the other hand, sperm DNA fragmentation, as measured by TUNEL assay, increased up to 23% by day 15 after the fever but this was not statistically significant.
  
  
  CONCLUSIONS
  This report demonstrates that a febrile episode can have marked effects on semen parameters and sperm DNA integrity. These results are particularly important for the counseling of infertile couples and in relation to assisted reproductive techniques (ART).
authors: Human Fertility Research Group, Université Toulouse III Paul Sabatier (EA 3694), Toulouse, France.
authors: Martin
authors: M
authors: Sergerie
authors: Roger
authors: R
authors: Mieusset
authors: Françoise
authors: F
authors: Croute
authors: Myriam
authors: M
authors: Daudin
authors: Louis
authors: L
authors: Bujan
mesh_terms: D002843:Chromatin / Q000737:chemistry; D053938:DNA Fragmentation; D005334:Fever / Q000150:complications*; D005434:Flow Cytometry; D006801:Humans; D020287:In Situ Nick-End Labeling; D007251:Influenza, Human / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D012661:Semen / Q000502:physiology*; D013076:Sperm Count; D013081:Sperm Motility
chemical_list: D002843:Chromatin