Selective photo-cross-linking of 2'-O-psoralen-conjugated oligonucleotide with RNAs having point mutations.
It has been reported that point mutations in genes are responsible for various cancers and the selective regulation of the gene expression is an important issue to develop a new type of anticancer drugs. In this report, we present a new type of antisense molecule that photo-cross-links to an oligoribonucleotide having a point mutation site in a sequence specific manner. 2'-O-psoralen-conjugated adenosine was synthesized in four steps from adenosine and introduced in the middle of an oligodeoxyribonucleotide (2'-Ps-oligo). Compared with 5'-O-psoralen-conjugated oligodeoxyribonucleotide (5'-Ps-oligo), which has a psoralen at the 5'-end, 2'-Ps-oligo more selectively photo-cross-linked to a pyrimidine base of the site of alteration from purine to pyrimidine in the oligoribonucleotide.
Higuchi
Maiko
M
Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Kyoto, Japan.
Yamayoshi
Asako
A
Yamaguchi
Takasei
T
Iwase
Reiko
R
Yamaoka
Tetsuji
T
Kobori
Akio
A
Murakami
Akira
A
D002621:Chemistry / Q000379:methods; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000494:pharmacology*; D005363:Ficusin / Q000737:chemistry*; D008027:Light; D008956:Models, Chemical; D009841:Oligonucleotides / Q000737:chemistry*; D016376:Oligonucleotides, Antisense / Q000737:chemistry; D017354:Point Mutation; D012313:RNA / Q000737:chemistry* / Q000235:genetics*; D013696:Temperature; D014466:Ultraviolet Rays
D003432:Cross-Linking Reagents; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; D012313:RNA; D005363:Ficusin