title: [Prolonged hypoxia induces GST-pi expression and drug resistance in lung adenocarcinoma cell line SPCA1].
abstract: OBJECTIVE
  To investigate the association of hypoxia with GST-pi expression in lung adenocarcinoma cells SPCA1 and the drug resistance in hypoxic condition.
  
  
  METHODS
  RNA and protein in SPCA1 cells treated with hypoxia (0.5% O(2)) for 16 h were isolated. HIF-1alpha and GST-pi mRNA expression was detected by real time RT-PCR, and HIF-1alpha protein was detected by Western blotting analysis. GST-pi protein was finally analyzed on a FACScan flow cytometer using Cellquest software. Sensitivity of Doxorubicin and Mitomycin in hypoxic SPCA1 cells was detected using cell clonogenic assay.
  
  
  RESULTS
  HIF-1alpha expression was up-regulated on mRNA and protein levels in hypoxic SPCA1 cells, so as was GST-pi expression. Doxorubicin resistance was increased, but hypoxia had no significant influence on Mitomycin resistance. GST-pi expression had no significant change after HIF-1alpha was down-regulated by RNA interfering.
  
  
  CONCLUSIONS
  Though prolonged hypoxia can induce GST-pi expression and increase drug resistance such as to Doxorubicin, HIF-1alpha expression has no correlation with GST-pi expression.
authors: Center of Cancer Research, Shandong Tumor Hospital and Institute, Jinan 250117, China. liu-yonglei@163.com
authors: Yong-lei
authors: YL
authors: Liu
authors: Xian-rang
authors: XR
authors: Song
authors: Xing-wu
authors: XW
authors: Wang
authors: Ling
authors: L
authors: Wei
authors: Da-peng
authors: DP
authors: Li
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism*; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm; D015251:Epirubicin / Q000494:pharmacology; D051549:Glutathione S-Transferase pi / Q000378:metabolism*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism; D008175:Lung Neoplasms / Q000378:metabolism*; D016685:Mitomycin / Q000494:pharmacology; D010957:Plasmids; D014162:Transfection
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D015251:Epirubicin; D016685:Mitomycin; D051549:Glutathione S-Transferase pi