In vitro evaluation and targeting of E. coli internalized by endothelial cells using albumin microspheres loaded with gentamicin.
The purpose of this study was to evaluate the use of microencapsulated form of gentamicin and the traditional solution form for its intracellular bactericidal effect. Bovine serumalbumin (BSA) microspheres loaded with gentamicin were prepared by using Mini Spray Dryer. Human microvascular endothelial cells (HMECs) were exposed to increasing concentrations of Escherichia coli leading to internalization of E. coli. The internalized bacteria were targeted using either the microencapsulated or the solution form of gentamicin. The treatment groups using gentamicin solution form and microsphere form showed almost 46% and 86% inhibition in the growth of the internalized bacteria, respectively.
Haswani
D K
DK
Department of Pharmaceutical Sciences, Southern School of Pharmacy, Mercer University, Atlanta, GA, USA. dkhaswani@gmail.com
Nettey
H
H
D'Souza
M J
MJ
Oettinger
C W
CW
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D002460:Cell Line; D015169:Colony Count, Microbial / Q000379:methods; D015638:Cytochalasin D / Q000494:pharmacology; D004339:Drug Compounding / Q000379:methods; D004705:Endocytosis / Q000187:drug effects; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000382:microbiology; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D005839:Gentamicins / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D006801:Humans; D007424:Intracellular Fluid / Q000187:drug effects / Q000382:microbiology; D008856:Microscopy, Fluorescence / Q000379:methods; D008863:Microspheres; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D010316:Particle Size; D015203:Reproducibility of Results; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013997:Time Factors
D000900:Anti-Bacterial Agents; D005839:Gentamicins; D019384:Nucleic Acid Synthesis Inhibitors; D015638:Cytochalasin D; D012710:Serum Albumin, Bovine