title: Diminished intestinal colonization by Clostridium difficile and immune response in mice after mucosal immunization with surface proteins of Clostridium difficile.
abstract: Clostridium difficile pathogenesis is mainly due to toxins A and B. However, the first step of pathogenesis is the colonization process. We evaluated C. difficile surface proteins as vaccine antigens to diminish intestinal colonization in a human flora-associated mouse model. First, we used the flagellar cap protein FliD of C. difficile, in order to test several immunization routes: intranasal, rectal, and intragastric. The rectal route, which is the most efficient, was used to vaccine groups of mice with different antigen combinations. After immunizations, the mice were challenged with the toxigenic C. difficile and a significant statistical difference between the control group and the immunized groups was observed in the colonization levels of C. difficile.
authors: Université de Paris-Sud, Faculté de Pharmacie, EA 4043, USC INRA, 5 rue JB Clement, F-92296 Châtenay-Malabry cedex, France. severine.pechine@u-psud.fr
authors: Séverine
authors: S
authors: Péchiné
authors: Claire
authors: C
authors: Janoir
authors: Hélène
authors: H
authors: Boureau
authors: Aude
authors: A
authors: Gleizes
authors: Nicolas
authors: N
authors: Tsapis
authors: Sandra
authors: S
authors: Hoys
authors: Elias
authors: E
authors: Fattal
authors: Anne
authors: A
authors: Collignon
mesh_terms: D000285:Administration, Rectal; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000276:immunology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000276:immunology; D002772:Cholera Toxin / Q000276:immunology / Q000494:pharmacology; D016360:Clostridioides difficile / Q000254:growth & development* / Q000276:immunology; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000276:immunology; D004761:Enterocolitis, Pseudomembranous / Q000276:immunology* / Q000382:microbiology*; D005260:Female; D006801:Humans; D018928:Immunity, Mucosal / Q000276:immunology; D007070:Immunoglobulin A / Q000096:biosynthesis / Q000276:immunology; D007422:Intestines / Q000276:immunology / Q000382:microbiology*; D051379:Mice; D008809:Mice, Inbred C3H
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D007070:Immunoglobulin A; C076733:FlaD protein, Bacteria; D002772:Cholera Toxin; D003546:Cysteine Endopeptidases