Eosinophil infiltration and activation in nasal polyposis.
Conclusions. Infiltration and activation of eosinophils is a characteristic of nasal polyposis. Allergic reaction is a risk factor for the accumulation of eosinophils in this disease. T-cell-derived interleukin 5 (IL-5) and autosecretion of IL-5 from activated eosinophils may be causative reasons for the extension and persistence of eosinophil inflammation. Objectives. To investigate whether eosinophils were accumulated and activated in nasal polyposis, and the roles of IL-5, eotaxin, and T cells in this process. Materials and methods. A retrospective study was conducted on 17 tissue samples from patients with nasal polyposis with allergy and 26 cases of non-allergic polyposis. Immunohistochemical staining by specific antibodies was carried out using the alkaline phosphatase anti-alkaline phosphatase method and the avidin-biotin complex technique.Results. The number of EG1-positive cells (pan eosinophil marker) was similar to the number of EG2-positive cells (activated eosinophil marker) in all tissue samples, although EG1- and EG2-positive cells were richer in allergic patients than those in non-allergic patients. Both EG1- and EG2-positive cells were correlated with CD3-positive cells (pan T cell marker) and IL-5-producing cells in allergic or non-allergic polyposis. A large proportion of IL-5 producing cells were eosinophils. Eotaxin protein was detected in all tissue samples and dominantly located in epithelial cells. Eotaxin expression between allergic and non-allergic subjects was not significantly different.
Fan
Guo-Kang
GK
Department of Otorhinolaryngology, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Jie-fang Road 88, Hangzhou, Zhejiang 310009, China.
Wang
Hualin
H
Takenaka
Hiroshi
H
D000293:Adolescent; D000328:Adult; D000368:Aged; D054413:Chemokine CCL11; D019742:Chemokines, CC / Q000032:analysis; D047091:Eosinophil Granule Proteins / Q000032:analysis; D004802:Eosinophilia / Q000276:immunology / Q000473:pathology*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D015848:Interleukin-5 / Q000032:analysis; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000276:immunology / Q000473:pathology; D009298:Nasal Polyps / Q000276:immunology / Q000473:pathology*; D012189:Retrospective Studies; D012221:Rhinitis, Allergic, Perennial / Q000276:immunology / Q000473:pathology; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology / Q000473:pathology; D012852:Sinusitis / Q000276:immunology / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology
C515456:CCL11 protein, human; D054413:Chemokine CCL11; D019742:Chemokines, CC; D047091:Eosinophil Granule Proteins; D015848:Interleukin-5