Comparative analysis of cell surface proteins in chronic and acute leukemia cell lines.
This study was designed to identify the cell surface protein markers that can differentiate between chronic myeloid leukemia (CML) and acute promyelocytic leukemia cells (APL). The differentially expressed plasma membrane proteins were analyzed between CML cell line (K562) and APL cell line (NB4) using the comparative proteomic approach. The cell membrane proteins were enriched by labeling with a membrane-impermeable biotinylation reagent, sulfo-NHS-SS-Biotin, and subjected to liquid chromatography tandem mass spectrometry (LC-MS/MS). By comparative proteomic analysis of K562 and NB4 cells, we identified 25 membrane and 14 membrane-associated proteins. The result of LC-MS/MS combined with chemical tagging method was validated by confirming the expression and localization of one of the differentially expressed plasma membrane proteins, CD43, by FACS and confocal microscopy. Our results indicate that CD43 could be a potential candidate for differentiating CML from APL.
Lee
Soo Jae
SJ
Department of Molecular Biotechnology, Institute of Biomedical Science and Technology, Konkuk University, Seoul 143-701, Republic of Korea.
Kim
Kyun-Hwan
KH
Park
Ji Sook
JS
Jung
Jin Woo
JW
Kim
Young Hwan
YH
Kim
Sang Kyung
SK
Kim
Wan-Seok
WS
Goh
Hyun-gyung
HG
Kim
Soo-hyun
SH
Yoo
Jung-Sun
JS
Kim
Dong-Wook
DW
Kim
Kwang Pyo
KP
D000595:Amino Acid Sequence; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D019921:Biotinylation; D045744:Cell Line, Tumor; D002853:Chromatography, Liquid; D003592:Cytophotometry / Q000379:methods; D003937:Diagnosis, Differential; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000378:metabolism / Q000473:pathology; D015473:Leukemia, Promyelocytic, Acute / Q000378:metabolism / Q000473:pathology; D051917:Leukosialin / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D013058:Mass Spectrometry / Q000379:methods; D008565:Membrane Proteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D018613:Microscopy, Confocal; D015203:Reproducibility of Results; D020539:Sequence Analysis, Protein
D014408:Biomarkers, Tumor; D005456:Fluorescent Dyes; D051917:Leukosialin; D008565:Membrane Proteins