Fungicidal monoclonal antibody C7 binds to Candida albicans Als3.
Monoclonal antibody (MAb) C7 reacted with a >200-kDa component from the Candida albicans cell wall identified by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry as Als3. It also bound the recombinant N terminus of Als3. Binding of MAb C7 to Als3 may explain the biological activities exerted by the MAb on C. albicans.
Brena
Sonia
S
Departamento de Inmunología, Microbiología y Parasitología, Facultad de Medicina y Odontología, Universidad del País Vasco, Apartado 699, E-48080 Bilbao, Vizcaya, Spain.
Omaetxebarría
Miren J
MJ
Elguezabal
Natalia
N
Cabezas
Jonathan
J
Moragues
María D
MD
Pontón
José
J
D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D002176:Candida albicans / Q000276:immunology* / Q000378:metabolism / Q000472:pathogenicity; D002473:Cell Wall / Q000737:chemistry* / Q000378:metabolism; D004847:Epithelial Cells / Q000382:microbiology; D018604:Epitope Mapping; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009061:Mouth Mucosa / Q000382:microbiology; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
C113620:ALS3 protein, Candida albicans; D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D005656:Fungal Proteins; D011994:Recombinant Proteins