Identification of disease-associated proteins by proteomic approach in ankylosing spondylitis.
Ankylosing spondylitis (AS) is a chronic systemic inflammatory disorder of the axial skeleton and shows significant inherited susceptibility. Auto-immune responses have been traditionally considered as a putative pathogenetic event in AS. However, no consistent self-antigen has been identified to responsible for the disorders in AS to this day. In this study, serum protein profiles of AS patients and healthy controls from a large Chinese AS family were investigated by two dimensional electrophoresis analysis. A group of four highly expressed protein spots was observed in all AS patients' profiles and subsequently identified as isoforms of haptoglobin precursor (pre-Hp) by ESI-Q-TOF MS/MS. Increased expression of haptoglobin precursor were also observed in sera of sporadic AS patients. Moreover, bioinformatics analysis revealed epitopes derived from haptoglobin precursor with high affinity binding to HLA-B( *)2705, a primary subtype associated with AS. These results indicate that pre-Hp may be involved in the pathogenesis of AS.
Liu
Jing
J
Center of Medical Genetics, Department of Hematology, Peking University First Hospital, Peking University, No. 8, Xishiku Street, West District, Beijing 100034, China.
Zhu
Ping
P
Peng
Jiarou
J
Li
Keqiu
K
Du
Jinwei
J
Gu
Jiangying
J
Ou
Yuan
Y
D000293:Adolescent; D000328:Adult; D015415:Biomarkers; D002648:Child; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D006242:Haptoglobins / Q000235:genetics*; D006579:Heterozygote; D006801:Humans; D008297:Male; D008875:Middle Aged; D010375:Pedigree; D020543:Proteome / Q000235:genetics*; D013167:Spondylitis, Ankylosing / Q000235:genetics*
D015415:Biomarkers; D006242:Haptoglobins; D020543:Proteome