title: In vitro biocompatibility of schwann cells on surfaces of biocompatible polymeric electrospun fibrous and solution-cast film scaffolds.
abstract: The in vitro responses of Schwann cells (RT4-D6P2T, a schwannoma cell line derived from a chemically induced rat peripheral neurotumor) on various types of electrospun fibrous scaffolds of some commercially available biocompatible and biodegradable polymers, i.e., poly(3-hydroxybutyrate) (PHB), poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), polycaprolactone (PCL), poly(l-lactic acid) (PLLA), and chitosan (CS), were reported in comparison with those of the cells on corresponding solution-cast film scaffolds as well as on a tissue-culture polystyrene plate (TCPS), used as the positive control. At 24 h after cell seeding, the viability of the attached cells on the various substrates could be ranked as follows: PCL film > TCPS > PCL fibrous > PLLA fibrous > PHBV film > CS fibrous approximately CS film approximately PLLA film > PHB film > PHBV fibrous > PHB fibrous. At day 3 of cell culture, the viability of the proliferated cells on the various substrates could be ranked as follows: TCPS > PHBV film > PLLA film > PCL film > PLLA fibrous > PHB film approximately PCL fibrous > CS fibrous > CS film > PHB fibrous > PHBV fibrous. At approximately 8 h after cell seeding, the cells on the flat surfaces of all of the film scaffolds and that of the PCL nanofibrous scaffold appeared in their characteristic spindle shape, while those on the surfaces of the PHB, PHBV, and PLLA macrofibrous scaffolds also appeared in their characteristic spindle shape, but with the cells being able to penetrate to the inner side of the scaffolds.
authors: Technological Center for Electrospun Fibers, The Petroleum and Petrochemical College, Chulalongkorn University, Phyathai Road, Pathumwan, Bangkok 10330, Thailand.
authors: Pakakrong
authors: P
authors: Sangsanoh
authors: Suchada
authors: S
authors: Waleetorncheepsawat
authors: Orawan
authors: O
authors: Suwantong
authors: Patcharaporn
authors: P
authors: Wutticharoenmongkol
authors: Oratai
authors: O
authors: Weeranantanapan
authors: Boontharika
authors: B
authors: Chuenjitbuntaworn
authors: Poonlarp
authors: P
authors: Cheepsunthorn
authors: Prasit
authors: P
authors: Pavasant
authors: Pitt
authors: P
authors: Supaphol
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology* / Q000633:toxicity; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D048271:Chitosan / Q000494:pharmacology / Q000633:toxicity; D006885:Hydroxybutyrates / Q000494:pharmacology / Q000633:toxicity; D019344:Lactic Acid / Q000494:pharmacology / Q000633:toxicity; D008422:Materials Testing; D011091:Polyesters / Q000494:pharmacology / Q000633:toxicity; D011108:Polymers / Q000494:pharmacology* / Q000633:toxicity; D000091402:Prohibitins; D051381:Rats; D012583:Schwann Cells / Q000187:drug effects* / Q000502:physiology; D012996:Solutions; D013499:Surface Properties; D000094122:Polyhydroxybutyrates
chemical_list: D001672:Biocompatible Materials; D048271:Chitosan; D006885:Hydroxybutyrates; D019344:Lactic Acid; D011091:Polyesters; D011108:Polymers; D000091402:Prohibitins; D012996:Solutions; C000717332:Phb protein, rat; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C016240:polycaprolactone; C003182:poly-beta-hydroxybutyrate; C033616:poly(lactide)