title: Shared MHC class II-dependent melanoma ribosomal protein L8 identified by phage display.
abstract: Antigens recognized by T helper (Th) cells in the context of MHC class II molecules have vaccine potential against cancer and infectious agents. We have described previously a melanoma patient's HLA-DR7-restricted Th cell clone recognizing an antigen, which is shared among melanoma and glioma cells derived from various patients. Here, this antigen was cloned using a novel antigen phage display approach. The antigen was identified as the ribosomal protein L8 (RPL8). A peptide of RPL8 significantly stimulated proliferation and/or cytokine expression of the Th cell clone and lymphocytes in four of nine HLA-DR7(+) melanoma patients but not in healthy volunteers. The RPL8 antigen may represent a relevant vaccine target for patients with melanoma, glioma, and breast carcinoma whose tumors express this protein.
authors: Immunology Program, The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA.
authors: Rolf K
authors: RK
authors: Swoboda
authors: Rajasekharan
authors: R
authors: Somasundaram
authors: Laura
authors: L
authors: Caputo
authors: Elizabeth M
authors: EM
authors: Ochoa
authors: Phyllis A
authors: PA
authors: Gimotty
authors: Francesco M
authors: FM
authors: Marincola
authors: Patricia
authors: P
authors: Van Belle
authors: Stephen
authors: S
authors: Barth
authors: David
authors: D
authors: Elder
authors: DuPont
authors: D
authors: Guerry
authors: Brian
authors: B
authors: Czerniecki
authors: Lynn
authors: L
authors: Schuchter
authors: Robert H
authors: RH
authors: Vonderheide
authors: Dorothee
authors: D
authors: Herlyn
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology*; D019556:COS Cells; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D015809:HLA-DR7 Antigen / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation; D008545:Melanoma / Q000235:genetics / Q000276:immunology*; D019151:Peptide Library; D012269:Ribosomal Proteins / Q000235:genetics / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
chemical_list: D000951:Antigens, Neoplasm; D000939:Epitopes; D015809:HLA-DR7 Antigen; D019151:Peptide Library; D012269:Ribosomal Proteins; C075184:ribosomal protein L8, rat