title: Neuroprotective effect of transcorneal electrical stimulation on the acute phase of optic nerve injury.
abstract: OBJECTIVE
  Traumatic optic neuropathy often induces a loss of vision that proceeds rapidly within several hours, together with retinal ganglion cell death, in a much slower time course. Electrical stimulation has previously been shown to rescue injured retinal ganglion cells from cell death. The present study tests whether transcorneal electrical stimulation could preserve visual function after an optic nerve crush.
  
  
  METHODS
  Transcorneal electrical stimulation was given immediately after a calibrated optic nerve crush. We measured visually evoked potentials (VEPs) in the visual cortex of rats before and immediately after the optic nerve crush and after the transcorneal stimulation to estimate an extent of damage and effects of stimulation in individual animals. In addition, the retinal axons were labeled with a fluorescent anterograde tracer to determine whether the transcorneal electrical stimulation can protect the retinal axons from degeneration.
  
  
  RESULTS
  The optic nerve crush was made at an intensity that does not allow a spontaneous recovery of VEP for 1 week. The transcorneal stimulation immediately increased VEP amplitude impaired by the optic nerve crush, and this augmentation was often preserved after 1 week. In the stimulated animals, a larger amount of retinal axons projected centrally beyond the crushed region in comparison to the unstimulated animals.
  
  
  CONCLUSIONS
  Transcorneal electrical stimulation would restore the functional impairment of optic nerve by traumatic injury at a very early stage and protect retinal axons from the ensuing degeneration.
authors: Division of Integrative Bioscience, Institute of Regenerative Medicine and Biofunction, Tottori University Graduate School of Medical Science, Yonago, Japan.
authors: Ken-ichiro
authors: K
authors: Miyake
authors: Miho
authors: M
authors: Yoshida
authors: Yoshitsugu
authors: Y
authors: Inoue
authors: Yoshio
authors: Y
authors: Hata
mesh_terms: D000208:Acute Disease; D000818:Animals; D001369:Axons / Q000502:physiology*; D003315:Cornea / Q000502:physiology; D004599:Electric Stimulation Therapy; D004594:Electrophysiology; D005074:Evoked Potentials, Visual / Q000502:physiology*; D009409:Nerve Crush; D009410:Nerve Degeneration / Q000503:physiopathology*; D020221:Optic Nerve Injuries / Q000503:physiopathology / Q000628:therapy*; D051381:Rats; D020318:Rats, Long-Evans; D012160:Retina / Q000502:physiology; D012165:Retinal Ganglion Cells / Q000502:physiology*; D014793:Visual Cortex / Q000502:physiology*