Effect of interferon-lambda on replication of hepatitis B virus in human hepatoma cells.
The antiviral activity of interferon-lambda (IFN-lambda) against several viruses has been reported, but it has not been investigated whether human IFN-lambda can affect replication of the hepatitis B virus (HBV) in human hepatocyte-derived cells. We found that replication of HBV in a human hepatoma cell line was reduced by approximately 30% following treatment with a high concentration of IFN-lambda. Furthermore, we showed that replication of HBV in another human hepatoma cell line was not suppressed by IFN-lambda. However, these cell lines showed a similar level of transcription for the heterodimeric IFN-lambda receptor and the IFN-lambda-induced antiviral proteins. Our results suggest that the antiviral activity of IFN-lambda against HBV may be limited in human cells.
Hong
Seung-Ho
SH
Department of Microbiology, Ajou University School of Medicine, Youngtongku Wonchondong San 5, Suwon 442-749, Republic of Korea.
Cho
Okki
O
Kim
Koyngmin
K
Shin
Ho-Joon
HJ
Kotenko
Sergei V
SV
Park
Sun
S
D000956:Antigens, Viral / Q000096:biosynthesis; D045744:Cell Line, Tumor; D016207:Cytokines / Q000494:pharmacology*; D015870:Gene Expression; D016679:Genome, Viral / Q000187:drug effects; D006515:Hepatitis B virus / Q000187:drug effects* / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D006801:Humans; D007372:Interferons; D007378:Interleukins / Q000494:pharmacology*; D017471:Receptors, Interferon / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects; D000095022:Interferon Lambda
D000956:Antigens, Viral; D016207:Cytokines; D007372:Interferons; D007378:Interleukins; D017471:Receptors, Interferon; D011994:Recombinant Proteins; C000624353:interferon-lambda, human; D000095022:Interferon Lambda