[Molecular diagnostic of parasites using rRNA gene sequence].
Ribosomal RNA (rRNA) is a component of the ribosomes. Eukaryotic ribosomes contain four different rRNA molecules: 18S, 5,8S, 28S and 5S rRNA. rRNA is the most conserved (least variable) gene in all cells. For this reason, genes that encode the rRNA (rDNA) are sequenced to identify an organism's taxonomic group, calculate related groups, and estimate rates of species divergence. Especially the internal transcribed spacers (ITS) are very useful for molecular diagnostic of parasite. They are noncoding regions of DNA sequence that separate genes coding for the 28S, 5.8S, and 18S ribosomal RNAs. These ribosomal RNA (rRNA) genes are highly conserved across taxa while the spacers between them may be species-specific. In this paper authors describe practical using of rRNA gene to parasite diagnostic.
Długosz
Ewa
E
Zakład Parazytologii i Inwazjologii, Wydział Medycyny Weterynaryjnej, SGGW, ul. Ciszewskiego 8, 02-786 Warszawa, Polska. ewa_plock@yahoo.com
Wiśniewski
Marcin
M
D000818:Animals; D001483:Base Sequence; D021901:DNA, Intergenic; D019143:Evolution, Molecular; D006801:Humans; D010271:Parasites / Q000145:classification* / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012335:RNA, Ribosomal / Q000032:analysis* / Q000737:chemistry; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014158:Transcription, Genetic
D021901:DNA, Intergenic; D012335:RNA, Ribosomal