Purification, characterization, gene cloning, and expression of a novel alcohol dehydrogenase with anti-prelog stereospecificity from Candida parapsilosis.
An alcohol dehydrogenase from Candida parapsilosis CCTCC M203011 was characterized along with its biochemical activity and structural gene. The amino acid sequence shows similarity to those of the short-chain dehydrogenase/reductases but no overall identity to known proteins. This enzyme with unusual stereospecificity catalyzes an anti-Prelog reduction of 2-hydroxyacetophenone to (S)-1-phenyl-1,2-ethanediol.
Nie
Yao
Y
Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Southern Yangtze University, Wuxi 214122, People's Republic of China.
Xu
Yan
Y
Mu
Xiao Qing
XQ
Wang
Hai Yan
HY
Yang
Ming
M
Xiao
Rong
R
D000426:Alcohol Dehydrogenase / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D002175:Candida / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013237:Stereoisomerism; D013379:Substrate Specificity
D004271:DNA, Fungal; D000426:Alcohol Dehydrogenase