Babesia bovis: the development of an expression oligonucleotide microarray.
The availability of a stage-specific Babesia bovis expression profile can facilitate the identification of candidate vaccine antigens. In addition, highly expressed genes during a particular developmental stage may suggest their relevance during that stage. In this study, we generated and validated a custom B. bovis high density oligonucleotide microarray that can be used to examine gene expression levels. An expression profile of in vitro cultured intraerythrocytic stage genes that could be distinguished from contaminating host message was established, and the expression levels of over 1000 genes were ranked. Ranking order was validated using quantitative real time PCR on a twelve randomly selected open reading frames whose expression levels range from the highest to the acceptable lowest. Expression of annotated ORFs was consistent with results from a recently published B. bovis expression sequence tag study. Therefore, we conclude that the microarray is suitable for analyzing B. bovis gene expression, and present the complete B. bovis infected erythrocyte expression profile.
Lau
Audrey O T
AO
Program in Microbial Genomics, Department of Veterinary Microbiology & Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, United States. laua@vetmed.wsu.edu
Tibbals
David L
DL
McElwain
Terry F
TF
D000818:Animals; D016793:Babesia bovis / Q000235:genetics*; D002417:Cattle; D020869:Gene Expression Profiling / Q000662:veterinary*; D020411:Oligonucleotide Array Sequence Analysis / Q000662:veterinary*; D016366:Open Reading Frames; D016053:RNA, Protozoan / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
D016053:RNA, Protozoan