title: Identification and quantification of dolichol and dolichoic acid in neuromelanin from substantia nigra of the human brain.
abstract: Neuromelanin (NM) isolated from the substantia nigra of the human brain is found to contain a series of dolichoic acids (dol-CA) containing 14-20 isoprene units. This is the first observation of dol-CA in a natural system. Using internally spiked nor-dolichol and nor-dolichoic acid standards, the concentrations of dolichol (dol) and dol-CA present in NM were determined. Remarkably, dol was only four times as abundant as dol-CA in NM. The distribution of dol-CA chains lengths in NM also differed from that of dol, suggesting that the enzyme(s) responsible for the conversion of dol to dol-CA prefer a dolichol substrate containing 19 isoprene units.
authors: Department of Chemistry, Duke University, Durham, NC, USA.
authors: Weslyn C
authors: WC
authors: Ward
authors: Ziqiang
authors: Z
authors: Guan
authors: Fabio A
authors: FA
authors: Zucca
authors: Ruggero G
authors: RG
authors: Fariello
authors: Reza
authors: R
authors: Kordestani
authors: Luigi
authors: L
authors: Zecca
authors: Christian R H
authors: CR
authors: Raetz
authors: John D
authors: JD
authors: Simon
mesh_terms: D000328:Adult; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004286:Dolichols / Q000032:analysis*; D006801:Humans; D008543:Melanins / Q000737:chemistry*; D013378:Substantia Nigra / Q000737:chemistry*; D034421:Sus scrofa; D053719:Tandem Mass Spectrometry; D013729:Terpenes / Q000032:analysis*
chemical_list: D004286:Dolichols; D008543:Melanins; D013729:Terpenes; C107327:dolichoic acid; C014121:neuromelanin