title: A microfluidic system for saliva-based detection of infectious diseases.
abstract: A "lab-on-a-chip" system for detecting bacterial pathogens in oral fluid samples is described. The system comprises: (1) an oral fluid sample collector; (2) a disposable, plastic microfluidic cassette ("chip") for sample processing including immunochromatographic assay with a nitrocellulose lateral flow strip; (3) a platform that controls the cassette operation by providing metered quantities of reagents, temperature regulation, valve actuation; and (4) a laser scanner to interrogate the lateral flow strip. The microfluidic chip hosts a fluidic network for cell lysis, nucleic acid extraction and isolation, PCR, and labeling of the PCR product with bioconjugated, upconverting phosphor particles for detection on the lateral flow strip.
authors: University of Pennsylvania School of Engineering and Applied Science, Philadelphia, Pennsylvania, USA.
authors: Zongyuan
authors: Z
authors: Chen
authors: Michael G
authors: MG
authors: Mauk
authors: Jing
authors: J
authors: Wang
authors: William R
authors: WR
authors: Abrams
authors: Paul L A M
authors: PL
authors: Corstjens
authors: R Sam
authors: RS
authors: Niedbala
authors: Daniel
authors: D
authors: Malamud
authors: Haim H
authors: HH
authors: Bau
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D002478:Cells, Cultured; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D006801:Humans; D056656:Lab-On-A-Chip Devices; D044085:Microfluidics / Q000295:instrumentation; D016133:Polymerase Chain Reaction; D012463:Saliva / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial