title: Phagocyte-derived oxidants and plasma antioxidants in haemodialysed patients.
abstract: OBJECTIVE
  Oxidative stress is one of the important complications occurring in haemodialysis. The aim of the study was to determine haemodialysis-induced changes in oxidative burst of phagocytes and the antioxidative properties of plasma.
  
  
  METHODS
  Twenty-seven patients and 50 healthy controls were examined. Oxidative burst of phagocytes and plasma antioxidative potential were measured luminometrically. Concentrations of major plasma antioxidants (vitamin E, bilirubin and uric acid) were also determined.
  
  
  RESULTS
  Phagocyte chemiluminescence was higher in patients before haemodialysis compared with that in controls and decreased after haemodialysis compared with predialysis status. A significant increase in plasma antioxidative potential and uric acid was found in patients before haemodialysis. These parameters decreased after haemodialysis compared with both predialysis and control values.
  
  
  CONCLUSIONS
  The higher generation of phagocyte-derived oxidants and the decline in plasma antioxidative properties after haemodialysis confirm insufficient antioxidant defence in patients with chronic renal failure.
authors: Department of Clinical Biochemistry and Hematology, St. Ann's University Hospital, Masaryk University. Brno. Czech Republic.
authors: V
authors: V
authors: Soska
authors: M
authors: M
authors: Ciz
authors: L
authors: L
authors: Kubala
authors: D
authors: D
authors: Sobotova
authors: A
authors: A
authors: Lojek
mesh_terms: D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000032:analysis*; D001663:Bilirubin / Q000097:blood; D005260:Female; D005609:Free Radicals / Q000032:analysis; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000628:therapy*; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D016877:Oxidants / Q000097:blood*; D010084:Oxidation-Reduction; D010586:Phagocytes / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000009:adverse effects; D012016:Reference Values; D006435:Renal Dialysis; D012307:Risk Factors; D014527:Uric Acid / Q000097:blood; D014810:Vitamin E / Q000097:blood
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D016877:Oxidants; D017382:Reactive Oxygen Species; D014810:Vitamin E; D014527:Uric Acid; D001663:Bilirubin