title: Comparative analysis of cell surface proteins in chronic and acute leukemia cell lines.
abstract: This study was designed to identify the cell surface protein markers that can differentiate between chronic myeloid leukemia (CML) and acute promyelocytic leukemia cells (APL). The differentially expressed plasma membrane proteins were analyzed between CML cell line (K562) and APL cell line (NB4) using the comparative proteomic approach. The cell membrane proteins were enriched by labeling with a membrane-impermeable biotinylation reagent, sulfo-NHS-SS-Biotin, and subjected to liquid chromatography tandem mass spectrometry (LC-MS/MS). By comparative proteomic analysis of K562 and NB4 cells, we identified 25 membrane and 14 membrane-associated proteins. The result of LC-MS/MS combined with chemical tagging method was validated by confirming the expression and localization of one of the differentially expressed plasma membrane proteins, CD43, by FACS and confocal microscopy. Our results indicate that CD43 could be a potential candidate for differentiating CML from APL.
authors: Department of Molecular Biotechnology, Institute of Biomedical Science and Technology, Konkuk University, Seoul 143-701, Republic of Korea.
authors: Soo Jae
authors: SJ
authors: Lee
authors: Kyun-Hwan
authors: KH
authors: Kim
authors: Ji Sook
authors: JS
authors: Park
authors: Jin Woo
authors: JW
authors: Jung
authors: Young Hwan
authors: YH
authors: Kim
authors: Sang Kyung
authors: SK
authors: Kim
authors: Wan-Seok
authors: WS
authors: Kim
authors: Hyun-gyung
authors: HG
authors: Goh
authors: Soo-hyun
authors: SH
authors: Kim
authors: Jung-Sun
authors: JS
authors: Yoo
authors: Dong-Wook
authors: DW
authors: Kim
authors: Kwang Pyo
authors: KP
authors: Kim
mesh_terms: D000595:Amino Acid Sequence; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D019921:Biotinylation; D045744:Cell Line, Tumor; D002853:Chromatography, Liquid; D003592:Cytophotometry / Q000379:methods; D003937:Diagnosis, Differential; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000378:metabolism / Q000473:pathology; D015473:Leukemia, Promyelocytic, Acute / Q000378:metabolism / Q000473:pathology; D051917:Leukosialin / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D013058:Mass Spectrometry / Q000379:methods; D008565:Membrane Proteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D018613:Microscopy, Confocal; D015203:Reproducibility of Results; D020539:Sequence Analysis, Protein
chemical_list: D014408:Biomarkers, Tumor; D005456:Fluorescent Dyes; D051917:Leukosialin; D008565:Membrane Proteins