title: Redox modulation of the apoptogenic activity of thapsigargin.
abstract: Thapsigargin (THG), a selective inhibitor of endoplasmic reticulum (ER) Ca2+-ATPases, causes the rapid emptying of ER Ca2+; in some cell types, this is accompanied by apoptosis, whereas other cells maintain viability. In order to understand the molecular determinants of such a different behavior, we explored the role of oxygen versus nitrogen radicals, by analyzing the apoptogenic ability of THG in the presence of inhibitors of glutathione or nitric oxide (NO) synthesis, respectively. We observed that oxygen radicals play a sensitizing role whereas nitrogen radicals prevent THG-dependent apoptosis, showing that the apoptogenic effect of THG is redox sensitive.
authors: Dipartimento di Biologia, Universita' di Roma Tor Vergata, via Ricerca Scientifica, 1, 00133 Roma (I), Italy.
authors: Claudia
authors: C
authors: Cerella
authors: Simona
authors: S
authors: Coppola
authors: Maria
authors: M
authors: D'Alessio
authors: Milena
authors: M
authors: De Nicola
authors: Andrea
authors: A
authors: Magrini
authors: Antonio
authors: A
authors: Bergamaschi
authors: Lina
authors: L
authors: Ghibelli
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D006801:Humans; D010084:Oxidation-Reduction; D019284:Thapsigargin / Q000494:pharmacology*; D020298:U937 Cells
chemical_list: D019284:Thapsigargin