title: Subpicosecond UV spectroscopy of carbonmonoxy-myoglobin: absorption and circular dichroism studies.
abstract: A thorough absorption and circular dichroism study is performed in carbonmonoxy-myoglobin with a sub-picosecond visible pump, ultraviolet probe experiment. Differential absorption in the 220-360 nm range shows that the time-resolved response mainly comes from the heme and that aromatic amino acids do not contribute significantly. Time-resolved CD at 260 nm shows no dynamics and confirms this result. On the contrary, a strong CD dynamics is observed at 230 nm. This signal could originate from transient deformation of the alpha-helices in the protein.
authors: Laboratoire d'Optique et Biosciences, Ecole Polytechnique, CNRS, INSERM, 91128 Palaiseau cedex, France.
authors: Thibault
authors: T
authors: Dartigalongue
authors: Claire
authors: C
authors: Niezborala
authors: François
authors: F
authors: Hache
mesh_terms: D000042:Absorption; D002942:Circular Dichroism / Q000379:methods; D009211:Myoglobin / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
chemical_list: D009211:Myoglobin; C018013:carboxymyoglobin