Plasmonic nanosensors for imaging intracellular biomarkers in live cells.
Here we present the first intracellular molecular imaging platform using multifunctional gold nanoparticles which incorporate both cytosolic delivery and targeting moieties on the same particle. The utility of these intracellular sensors was demonstrated by monitoring actin rearrangement in live fibroblasts. We observed a strong molecular specific optical signal associated with effective targeting of actin filaments. These multifunctional nanosensors can be adapted to target various intracellular processes especially where transfection or cytotoxic labels are not feasible.
Kumar
Sonia
S
Department of Biomedical Engineering, University of Texas at Austin, Austin, Texas 78712, USA.
Harrison
Nathan
N
Richards-Kortum
Rebecca
R
Sokolov
Konstantin
K
D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D015374:Biosensing Techniques; D005347:Fibroblasts / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles; D051379:Mice; D046529:Microscopy, Electron, Transmission; D041681:NIH 3T3 Cells
D015415:Biomarkers; D006046:Gold