title: Characterization of the antibody isotype response in serum and milk of heifers vaccinated with a Staphylococcus aureus bacterin (Lysigin).
abstract: The objective was to characterize the antibody isotype responses to vaccination with a commercial Staphylococcus aureus bacterin in dairy heifers. Twenty-five Holstein-Friesian dairy heifers were assigned at random to one of two groups, vaccinates (n=14) or controls (n=11). Vaccinates received two 5-ml doses of Lysigin 28 d apart in late gestation. Both groups were challenged with a heterologous serotype 5 strain of Staph. aureus by aseptic intramammary infusion on days 6, 7 and 8 of lactation. Samples for serum antibody isotype (IgG, IgG1, IgG2 and IgM) determinations were taken before each vaccination, immediately prior to challenge (6 d post-calving) and at the end of the study (28 d post-calving). Samples for milk antibody isotype determinations were taken immediately prior to challenge and at the end of the study. Antibody isotype sample-to-positive ratios (S:P ratio=(mean test sample OD-mean within-plate negative control OD)/(mean within-plate positive control OD-mean within-plate negative control OD)) were determined in milk and serum using a series of ELISAs coated with different strains of Staph. aureus belonging to capsular polysaccharide (CP) serotype 5 or 8 or surface polysaccharide (SP) serotype 336. Vaccinates had higher mean serum IgG1 and IgG2 S:P ratios than controls against all three strains of Staph. aureus (P < or = 0.023). Vaccinates had higher mean milk IgG S:P ratios than controls against CP8 and SP336 strains of Staph. aureus (P< or = 0.030). Hence, a humoural immune response to the vaccine was detected in serum and milk, but responses varied according to strain and antibody isotype tested.
authors: Department of Veterinary Medicine and Surgery, University of Missouri, Columbia, USA. middletonjr@missouri.edu
authors: Christopher D
authors: CD
authors: Luby
authors: John R
authors: JR
authors: Middleton
authors: Jianneng
authors: J
authors: Ma
authors: Carol L
authors: CL
authors: Rinehart
authors: Scott
authors: S
authors: Bucklin
authors: Caroline
authors: C
authors: Kohler
authors: Jeff W
authors: JW
authors: Tyler
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology; D002417:Cattle; D005260:Female; D007132:Immunoglobulin Isotypes / Q000032:analysis* / Q000097:blood; D008892:Milk / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology*
chemical_list: D001428:Bacterial Vaccines; D007132:Immunoglobulin Isotypes