[Transformation of Dunaliella salina by using glass beads--a novel transformation method].
A novel transformation method was firstly established using glass beads in Dunaliella salina (D. salina). The results showed that the GUS gene, a reporter gene, was successfully expressed in D. salina. Cells of D. salina presented blue color under the microscope after stained. In addition, different factors which influenced transformation were optimized including the transformation consecutive time, rotate speed, concentration of the plasmid and PEG 6000. The experiment indicated that this fit together can obtain the best results for D. salina transformation: adding 150 microL PEG and 90 microL plasmid DNA to 800 microL culture of D. salina (10(6) cells/mL) containing 300 mg glass beads, swirling 12 seconds under the rotate speed 2400r/min. This newly method can be used as a potential tool in the research of D. salina gene engineering with the advantage of more simpleness, convenience, quickness and less expense.
Feng
Shu-Ying
SY
Laboratory Center for Medical Sciences, The First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China.
Jia
Yan-Long
YL
Liu
Hong-Tao
HT
Li
Jie
J
Xue
Le-Xun
LX
D000460:Chlorophyta / Q000235:genetics*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods; D005898:Glass; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D006651:Histocytochemistry; D008863:Microspheres; D010957:Plasmids / Q000235:genetics; D011092:Polyethylene Glycols / Q000737:chemistry; D013997:Time Factors; D014170:Transformation, Genetic / Q000235:genetics*
D011092:Polyethylene Glycols; D004247:DNA; D005966:Glucuronidase