High doses of ultraviolet-C irradiation increases vasoactive intestinal contractor/endothelin-2 expression in keratinocytes of the newborn mouse epidermis.
We examined the expression profiles of vasoactive intestinal contractor/endothelin-2 (VIC/ET-2) at both gene and peptide level in skin irradiated with different ultraviolet wavelengths. We found that VIC/ET-2 gene expression is sensitive only to ultraviolet-C (UVC) irradiation and has an immediate response. These results provide direct evidence that high doses of UVC irradiation induce an increase in gene expression and protein production of VIC/ET-2 and endothelin (ET) receptors in a dose-dependent manner in epidermal keratinocytes. We suggest that VIC/ET-2 can play an essential role in the maintenance, protection and hyperpigmentation of the epidermis exposed to UVC irradiation from artificial or natural sources.
Adur
Javier
J
National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8566, Japan. jadur@bioingenieria.edu.ar
Takizawa
Satoshi
S
Uchide
Tsuyoshi
T
Casco
Victor
V
Saida
Kaname
K
D000818:Animals; D000831:Animals, Newborn; D004307:Dose-Response Relationship, Radiation; D019333:Endothelin-2 / Q000235:genetics* / Q000378:metabolism; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism / Q000528:radiation effects*; D015870:Gene Expression / Q000528:radiation effects*; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010455:Peptides / Q000235:genetics* / Q000378:metabolism; D017466:Receptors, Endothelin / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014466:Ultraviolet Rays
D019333:Endothelin-2; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D017466:Receptors, Endothelin; C059568:vasoactive intestinal constrictor