Plasmodium berghei XAT: protective 155/160 kDa antigens are located in parasitophorous vacuoles of schizont-stage parasite.
Effective blood-stage malaria vaccine candidates have been mainly developed from the proteins in exposed locations on the parasite such as the surface of free merozoites or infected red blood cells. In the present study, we identified and localized novel protective antigens derived from the blood-stage of Plasmodium berghei XAT after establishment of hybridomas producing protective monoclonal antibodies (mAbs) against the parasites. The protective antigens were expressed in schizonts but not in trophozoites, and located in the parasitophorous vacuoles in the infected erythrocyte cytoplasm. The antigens, with molecular weight of 155/160 kDa, were not identical to any merozoite/schizont antigens that have been reported as target molecules recognized by mAbs developed to rodent malaria parasites. The characterization of new malarial antigenic targets of potentially protective antibody responses following infection would give us new insights for the selection of candidate antigens for malaria vaccine.
Kobayashi
Fumie
F
Department of Infectious Diseases, Kyorin University School of Medicine, Mitaka, Tokyo 181-8611, Japan. fumfum@kyorin-u.ac.jp <fumfum@kyorin-u.ac.jp>
Waki
Seiji
S
Niikura
Mamoru
M
Tachibana
Mayumi
M
Tsuboi
Takafumi
T
Torii
Motomi
M
Kamiya
Shigeru
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000627:therapeutic use; D000953:Antigens, Protozoan / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis / Q000737:chemistry / Q000276:immunology; D004912:Erythrocytes / Q000469:parasitology; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006825:Hybridomas; D007116:Immunization, Passive; D008288:Malaria / Q000276:immunology / Q000517:prevention & control*; D017780:Malaria Vaccines / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016253:Microscopy, Immunoelectron; D008970:Molecular Weight; D018512:Parasitemia / Q000276:immunology / Q000517:prevention & control; D010962:Plasmodium berghei / Q000276:immunology*; D014613:Vaccines, Attenuated / Q000276:immunology; D014617:Vacuoles / Q000276:immunology
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D000939:Epitopes; D017780:Malaria Vaccines; D014613:Vaccines, Attenuated