title: Transfection and expression of plasmid DNA in plant cells by an arginine-rich intracellular delivery peptide without protoplast preparation.
abstract: The delivery and expression of exogenous genes in plant cells have been of particular interest for plant research and biotechnology. Here, we present results demonstrating a simple DNA transfection system in plants. Short arginine-rich intracellular delivery peptide, a protein transduction domain, was capable of delivering plasmid DNA into living plant cells non-covalently. This peptide-mediated DNA delivery conferred several advantages, such as nuclear targeting, non-toxic effect, and ease of preparation without protoplast formulation. Thus, this novel technology shall provide a powerful tool to investigate gene function in vivo, and lay the foundation for the production of transgenic plants in future.
authors: Department of Life Science, National Dong Hwa University, Hualien 97401, Taiwan.
authors: Chung-Pin
authors: CP
authors: Chen
authors: Jyh-Ching
authors: JC
authors: Chou
authors: Betty Revon
authors: BR
authors: Liu
authors: Microsugar
authors: M
authors: Chang
authors: Han-Jung
authors: HJ
authors: Lee
mesh_terms: D001120:Arginine / Q000737:chemistry; D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors / Q000737:chemistry; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D010944:Plants / Q000235:genetics*; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000378:metabolism; D011523:Protoplasts / Q000378:metabolism*; D013997:Time Factors; D014162:Transfection / Q000379:methods*
chemical_list: D010455:Peptides; D001120:Arginine