Photochemical DNA activation.
A new photocaged nucleoside was synthesized and incorporated into DNA with the use of standard synthesis conditions. This approach enabled the disruption of specific H-bonds and allowed for the analysis of their contribution to the activity of a DNAzyme. Brief irradiation with nonphotodamaging UV light led to rapid decaging and almost quantitative restoration of DNAzyme activity. The developed strategy has the potential to find widespread application in the light-induced regulation of oligonucleotide function.
Lusic
Hrvoje
H
Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA.
Young
Douglas D
DD
Lively
Mark O
MO
Deiters
Alexander
A
D021881:DNA, Catalytic / Q000737:chemistry* / Q000378:metabolism*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010777:Photochemistry; D012313:RNA / Q000378:metabolism; D013379:Substrate Specificity
D021881:DNA, Catalytic; D012313:RNA