Immunoassay-based diagnostic point-of-care technology for oral specimen.
We have outlined our progress in developing a novel point-of-care platform to quantify micro-organisms causing dental infections and/or inflammatory markers reflecting an oral disease status. This system is based on a sandwich immunoassay technology known as ABICAP (Antibody Immuno Column for Analytical Processes) using poly-horseradish peroxidase conjugates. This assay enabled us to quantify 500 colony-forming units of Streptococcus sobrinus per milliliter of saliva. The platform allows rapid and convenient performance chairside of such tests by a dentist or dental hygienist within 20 minutes at the dental office.
Munjal
Sarvan
S
Dentognostics GmbH, Jena, Germany.
Miethe
Peter
P
Netuschil
Lutz
L
Struck
Friedhelm
F
Maier
Kurt
K
Bauermeister
Claus
C
D015415:Biomarkers; D003731:Dental Caries / Q000175:diagnosis* / Q000382:microbiology; D005883:Gingival Crevicular Fluid / Q000737:chemistry / Q000382:microbiology; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation; D019095:Point-of-Care Systems; D012463:Saliva / Q000737:chemistry / Q000382:microbiology; D013290:Streptococcal Infections / Q000175:diagnosis / Q000382:microbiology; D013295:Streptococcus mutans / Q000302:isolation & purification; D016986:Streptococcus sobrinus / Q000302:isolation & purification
D015415:Biomarkers