Isolation and molecular characterization of a porcine teschovirus 1 isolate from China.
Porcine teschovirus 1 (PTV-1) (Swine/CH/IMH/03) was isolated from piglets in a farm in Inner Mongolia Province, P.R. China. It was confirmed by electron microscopy, RT-PCR, and sequencing. Comparison of the sequences of the amino acid and nucleotides and phylogenetic analysis of the polyprotein showed that PTV Swine/CH/IMH/03 strain is PTV-1. The isolated virus has closest relationship with Talfan strain, they shared 98.9% and 99.5% homology of amino acids and nucleotides, respectively, in the ORF of polyprotein. To our knowledge, this is the first report about isolation and identification of a PTV in China.
Feng
L
L
Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. fengli_h@163.com
Shi
H Y
HY
Liu
S W
SW
Wu
B P
BP
Chen
J F
JF
Sun
D B
DB
Tong
Y E
YE
Fu
M S
MS
Wang
Y F
YF
Tong
G Z
GZ
D000818:Animals; D000829:Animals, Domestic; D001921:Brain / Q000821:virology; D020805:Central Nervous System Viral Diseases / Q000662:veterinary / Q000821:virology; D002681:China; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D010802:Phylogeny; D010850:Picornaviridae Infections / Q000662:veterinary* / Q000821:virology; D020815:Polyproteins / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D052700:Teschovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000648:ultrastructure; D014764:Viral Proteins / Q000235:genetics
D020815:Polyproteins; D012367:RNA, Viral; D014764:Viral Proteins