title: Lithium microdialysis and its use for monitoring of stomach and colon submucosal blood perfusion--a pilot study using ischemic preconditioning in rats.
abstract: During shock, exposure of gut to ischemia determines patient's survival. Ischemic preconditioning (ISP) elevates nitric oxide and blood perfusion, whereby it protects organs against subsequent severe ischemia/reperfusion. Using appropriate flow marker, microdialysis may serve to monitor interstitial microcirculation. Hence, our aim was to test the reliability of lithium as a flow marker (lithium microdialysis, LM) on an ISP model. Rats were divided into three groups. Two (ischemic and preconditioned) groups underwent 30 min celiac artery occlusion (CAO) with 2.5 h reperfusion. 25 min before CAO, the latter experienced 5 min ischemia. Sham-operated animals served as controls. LM in stomach and colon submucosa, serum nitric oxide, hepatic and pancreatic enzymes were measured. In stomach, LM indicated a decrease in blood perfusion evoked by CAO (p < 0.01) in both experimental groups. During reperfusion, the ischemic animals showed a restoration of microcirculation, unlike the preconditioned ones, whose blood perfusion failed to regenerate (p < 0.001). For any group, LM showed no microcirculation modification in colon. Serum analytes remained unchanged. We conclude that LM appears to be a potentially suitable indicator of gastrointestinal interstitial microcirculation. However, we failed to demonstrate any beneficial effect of ISP on pancreas, systemic nitric oxide and local/remote microcirculation within studied organs.
authors: Charles University in Prague, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Czech Republic. cibicekn@lfhk.cuni.cz
authors: Norbert
authors: N
authors: Cibicek
authors: Stanislav
authors: S
authors: Micuda
authors: Jaroslav
authors: J
authors: Chládek
authors: Pavel
authors: P
authors: Zivný
authors: Zdenek
authors: Z
authors: Zadák
authors: Eva
authors: E
authors: Cermáková
authors: Vladimír
authors: V
authors: Palicka
mesh_terms: D000818:Animals; D003106:Colon / Q000098:blood supply*; D005753:Gastric Mucosa / Q000098:blood supply*; D007413:Intestinal Mucosa / Q000098:blood supply*; D007511:Ischemia / Q000097:blood / Q000503:physiopathology; D019194:Ischemic Preconditioning; D008094:Lithium; D008099:Liver / Q000201:enzymology; D008297:Male; D008833:Microcirculation; D017551:Microdialysis; D009569:Nitric Oxide / Q000097:blood; D010179:Pancreas / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D013270:Stomach / Q000098:blood supply*
chemical_list: D009569:Nitric Oxide; D008094:Lithium