title: Multi-walled carbon nanotube-based glucose/O2 biofuel cell with glucose oxidase and laccase as biocatalysts.
abstract: This study describes a multi-walled carbon nanotube-based glucose/O2 biofuel cell with glucose oxidase and laccase as the anodic and cathodic biocatalysts, respectively. Upon being functionalized with L-a-phosphatidylcholine, one kind of lipid, multi-walled carbon nanotubes can serve as a support for glucose oxidase to form a three-dimensional, conducting and uniform bioanode that possesses a good bioelectrocatalytic activity toward the oxidation of glucose biofuel with solution-phased ferrocene monocarboxylic acid as the mediator to shuttle the electron transfer between glucose oxidase and multi-walled carbon nanotubes. In a similar way, the lipid-functionalized multiwalled carbon nanotubes can also be used to support the cathodic biocatalyst, i.e., laccase, and, more remarkably, to facilitate the direct electron transfer of laccase. As a result, the prepared biocathode is very active toward the reduction of oxygen without using any electron-transfer mediators. The biofuel cell has a 0.45 V open circuit potential and 34 microA/cm2 short circuit current density in phosphate buffer (pH 6.0) separated with Nafion-117 membrane with anodic compartment containing 15 mM glucose and 2 mM ferrocene monocarboxylic acid and with cathodic compartment being saturated with O2 at room temperature. A maximum power density of 3.2 microW/cm2 is obtained with ca. 0.2 V potential output.
authors: Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, The Chinese Academy of Sciences, Beijing 100080, P. R. China.
authors: Yiming
authors: Y
authors: Yan
authors: Lei
authors: L
authors: Su
authors: Lanqun
authors: L
authors: Mao
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation; D002384:Catalysis; D004563:Electrochemistry / Q000379:methods*; D004583:Electrons; D005296:Ferrous Compounds / Q000737:chemistry; D005947:Glucose / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000737:chemistry*; D000075163:Metallocenes; D008956:Models, Chemical; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry*
chemical_list: D005296:Ferrous Compounds; D000075163:Metallocenes; D037742:Nanotubes, Carbon; C032949:ferrocenecarboxylic acid; D005949:Glucose Oxidase; D042845:Laccase; D005947:Glucose; D010100:Oxygen