Ferrocene branched chitosan for the construction of a reagentless amperometric hydrogen peroxide biosensor.
Chitosan was chemically branched with ferrocene moieties and further used as a support for the immobilization of horseradish peroxidase on a glassy carbon electrode. The reagentless biosensor device showed a linear amperometric response toward hydrogen peroxide concentrations between 35 x 10(-6) M and 2.0 x 10(-3) M. The biosensor reached 95% of the steady-state current in about 20 s and its sensitivity was 28.4 x 10(-3) microA x M(-1). The enzyme electrode retained 94% of its initial activity after 2 weeks of storage at 4 degrees C in 50 x 10(-3) M sodium phosphate buffer at pH 7.0.
Garcia
Ariel
A
Center for Enzyme Technology, University of Matanzas, Autopista a Varadero Km 3 1/2, Matanzas, C.P. 44740, Cuba.
Peniche-Covas
Carlos
C
Chico
Belkis
B
Simpson
Benjamin K
BK
Villalonga
Reynaldo
R
D015374:Biosensing Techniques; D048271:Chitosan / Q000737:chemistry*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D005296:Ferrous Compounds / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000032:analysis*; D000075163:Metallocenes
D004800:Enzymes, Immobilized; D005296:Ferrous Compounds; D000075163:Metallocenes; D048271:Chitosan; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; C004998:ferrocene