title: [Noninvasive detection of experimental vulnerable atherosclerotic plaques with 99Tc m labeled C2A domain of synaptotagmin I].
abstract: OBJECTIVE
  (99)Tc(m) labeled C2A domain of synaptotagmin I ((99)Tc(m)-Syt I-C2A) is used for noninvasive detection of vulnerable atherosclerotic plaque.
  
  
  METHODS
  Recombinant C2A domain of synaptotagmin I, overexpressed in E. Coli, was thiolated with 2-iminothiolane (2-IT) and labeled with (99)Tc(m). Atherosclerotic plaques were produced in 5 rabbits by deendothelialization of the abdominal aorta and the rabbits were fed with cholesterol diet for 3 months. Three rabbits not manipulated served as normal controls. All animals were injected with (99)Tc(m)-Syt I-C2A and underwent in vivo imaging thereafter. Aortas were then explanted for ex vivo imaging and histological characterization.
  
  
  RESULTS
  In deendothelialized animals, intense radio-uptake in abdominal aorta, showed by gamma camera at 2 h after injection, was visualized and T/B was 3.25 +/- 0.51 by ROI measurement, quantitative uptake ratio of abdominal aortas with atherosclerotic lesions to thoracic aortas was 8.39 +/- 1.74 in ex vivo imaging. The mean uptake in specimens of abdominal aortas with lesions was 12.6-fold higher than in control abdominal aortas, and 10.2-fold higher than in thoracic aortas of deendothelialized animals by gamma-counter.
  
  
  CONCLUSIONS
  (99)Tc(m)-Syt I-C2A has a high affinity for vulnerable atherosclerotic plaque and is a suitable a gent for the noninvasive detection of vulnerable atherosclerotic plaque.
authors: Department of Nuclear Medicine, Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China.
authors: Wei
authors: W
authors: Fang
authors: Shun-dong
authors: SD
authors: Ji
authors: Feng
authors: F
authors: Wang
authors: Min-fu
authors: MF
authors: Yang
authors: Bin
authors: B
authors: Lü
authors: Lei
authors: L
authors: Liu
authors: Zi-zheng
authors: ZZ
authors: Wang
authors: Zuo-xiang
authors: ZX
authors: He
mesh_terms: D000818:Animals; D050197:Atherosclerosis / Q000000981:diagnostic imaging* / Q000473:pathology; D004195:Disease Models, Animal; D007140:Immunoglobulin Fab Fragments; D007553:Isotope Labeling; D008297:Male; D011817:Rabbits; D011877:Radionuclide Imaging; D050863:Synaptotagmin I / Q000276:immunology; D013667:Technetium
chemical_list: D007140:Immunoglobulin Fab Fragments; D050863:Synaptotagmin I; D013667:Technetium