[Effect of Aurora A on biological phenotypes of prostate cancer cells].
OBJECTIVE
To examine the effect of Aurora A on the biological phenotypes of prostate cancer cells and the role of Aurora A in the carcinogenesis of prostate cancer.


METHODS
Full length Aurora A gene was cloned and transfected into the prostate cancer cell line LNCaP to construct a positive cell clone with high expression of Aurora A. The growth and proliferation of the cells was detected by MTT. The mobility of the cells was examined by transwell cell layer infiltration assay. The growth and motility of LNCaP-Aurora A cells were compared with those of the LNCaP-pcDNA3. 1/LNCaP cells.


RESULTS
Compared to the LNCaP-pcDNA3. 1/ LNCaP cells, the cells with high expression of Aurora A had faster proliferation and stronger invasive ability. Three days after cultivation, more LNCaP-Aurora A cells had grown than the LNCaP-pcDNA3. 1/LNCaP cells (P < 0.05).


CONCLUSIONS
The increased expression of Aurora A is closely associated with the malignant phenotypes of prostate cancer. Aurora A may play a very important role in the carcinogenesis of prostate cancer.
Zhao
Feng-Yan
FY
Experiment Center, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Qu
Yia
Y
Zhang
Lin
L
Mu
De-Zhi
DZ
Huang
Xiang
X
Wei
Da-Peng
DP
D000818:Animals; D064127:Aurora Kinases; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism*; D014162:Transfection
D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases