A model system for analyzing somatic mutations in Drosophila melanogaster.
Presently there are no good assays for comparing somatic mutation frequencies and spectra between different vertebrate and invertebrate organisms. Here we describe a new lacZ mutation reporter system in D. melanogaster, which complements existing systems in the mouse. The results obtained with the new model indicate two-to threefold higher frequencies of spontaneous mutations than in the mouse, with most of the mutations characterized as large genome rearrangements.
Garcia
Ana Maria
AM
University of Texas at San Antonio, San Antonio, Texas 78249, USA.
Derventzi
Anastasia
A
Busuttil
Rita
R
Calder
R Brent
RB
Perez
Ernesto
E
Chadwell
Linda
L
Dollé
Martijn E T
ME
Lundell
Martha
M
Vijg
Jan
J
D000818:Animals; D030801:Animals, Genetically Modified; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D007763:Lac Operon / Q000235:genetics*; D008297:Male; D008957:Models, Genetic; D009154:Mutation; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000378:metabolism
C090528:DNA modification methylase HindIII; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)