Rapid inhibitory effect of corticosterone on histamine release from rat peritoneal mast cells.
Glucocorticoids are steroids endowed with powerful anti-inflammatory properties, which are routinely believed to require several hours to take effect through modulation of gene expression. Our recent report has shown that glucocorticoids could inhibit allergic reaction within 10 minutes, which the classical genomic mechanism could not explain. Histamine is thought to be one of major mediators in the allergic reaction, and IgE-mediated histamine release from mast cells plays a pivotal role in allergic diseases. Here, we have determined a rapid effect of corticosterone on histamine release from rat peritoneal mast cells, using fluorometric assay. The results showed that corticosterone could inhibit antigen-induced histamine release from rat peritoneal cells within 15 minutes (p<0.05), which could be mimicked by membrane-impermeable BSA conjugated corticosterone (p<0.05). Neither glucocorticoid nuclear receptor antagonist nor protein synthesis inhibitor could block the rapid action (p<0.05). The study provided evidence that nongenomic mechanism might be involved in rapid effect of glucocorticoids on mast cells in allergic disease.
Liu
C
C
Laboratory of Stress Medicine, Second Military Medical University, Shanghai, PR China.
Zhou
J
J
Zhang
L-D
LD
Wang
Y-X
YX
Kang
Z-M
ZM
Chen
Y-Z
YZ
Jiang
C-L
CL
D000818:Animals; D003345:Corticosterone / Q000494:pharmacology*; D003470:Culture Media; D006636:Histamine Release / Q000187:drug effects*; D006727:Hormone Antagonists / Q000494:pharmacology; D007106:Immune Sera; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D015735:Mifepristone / Q000494:pharmacology; D010529:Peritoneal Cavity / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011965:Receptors, Glucocorticoid / Q000037:antagonists & inhibitors / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000494:pharmacology
D003470:Culture Media; D006727:Hormone Antagonists; D007106:Immune Sera; D011965:Receptors, Glucocorticoid; D012710:Serum Albumin, Bovine; D015735:Mifepristone; D003345:Corticosterone