title: Analysing protein-protein interaction networks of human liver cancer cell lines with diverse metastasis potential.
abstract: OBJECTIVE
  Human hepatocellular carcinoma (HCC) is one of the most mortal tumor. In a previous study, we had constructed glycoprotein expression profiles and glycoprotein databases of three human liver cancer cell lines with diverse metastasis potential. In order to discover vital glycoproteins related to pathogenesis and metastasis of HCC, in this study we analyzed previous data with bioinformatic approach.
  
  
  METHODS
  We took previous data to draw the protein-protein interaction (PPI) networks of liver cell lines by searching IntACT database and then using Pajeck software. Further more, we compared the differences between the three PPI networks by drawing the PPI networks of differential glycoproteins and by naming differential display PPI networks.
  
  
  RESULTS
  Large numbers of proliferation and apoptosis-relative proteins interact with the differential glycoproteins, and among the differential glycoproteins there are many interactions.
  
  
  CONCLUSIONS
  We conclude that neither single nor several proteins cause malignant proliferation of liver cells. "Molecule groups" concept should be introduced into diagnosis and metastasis prediction of the HCC.
authors: Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors: Hai-Jun
authors: HJ
authors: Zhou
authors: Yin-Kun
authors: YK
authors: Liu
authors: Zhuozhe
authors: Z
authors: Li
authors: Dong
authors: D
authors: Yun
authors: Qiang-Ling
authors: QL
authors: Shun
authors: Kun
authors: K
authors: Guo
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D019295:Computational Biology; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D008967:Molecular Biology; D009362:Neoplasm Metastasis / Q000473:pathology; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D006023:Glycoproteins