Effects of sulfhydryl compounds on cancer cell lines: I: N-(2-mercaptopropionyl)-glycine exerts antiproliferating effects and antagonizes the stimulating effect of prolactin on MCF-7 human breast cancer cells.
The effects of prolactin (PRL) and N-(2-mercaptopropionyl)-glycine (MPG) on the growth of MCF-7 human breast cancer cells, and the influence of MPG on PRL-induced MCF- 7 cell proliferation, when added simultaneously to the culture medium, were examined. Prolactin at concentrations of 200 ng/ml - 350 ng/ml enhanced the growth of MCF-7 cells, while at lower and higher concentrations its action was diminished. MPG alone at concentrations above 2 mM caused a decrease in cell proliferation. When PRL and MPG were added simultaneously to the culture medium, an inhibitory effect on PRL-induced MCF-7 cell proliferation was observed, at concentrations of MPG lower than 2 mM.
Malamas
F M
FM
Laboratory of Physiology, Faculty of Medicine, University of Ioannina, Ioannina, Greece. mmalamas@cc.uoi.gr
Thomas
C G
CG
Stefos
T
T
Evangelou
A
A
D001943:Breast Neoplasms; D002455:Cell Division / Q000187:drug effects*; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D011388:Prolactin / Q000494:pharmacology*; D013438:Sulfhydryl Compounds / Q000494:pharmacology*; D008625:Tiopronin / Q000494:pharmacology*
D013438:Sulfhydryl Compounds; D011388:Prolactin; D008625:Tiopronin