title: The nuclear-encoded factor HCF173 is involved in the initiation of translation of the psbA mRNA in Arabidopsis thaliana.
abstract: To gain insight into the biogenesis of photosystem II (PSII) and to identify auxiliary factors required for this process, we characterized the mutant hcf173 of Arabidopsis thaliana. The mutant shows a high chlorophyll fluorescence phenotype (hcf) and is severely affected in the accumulation of PSII subunits. In vivo labeling experiments revealed a drastically decreased synthesis of the reaction center protein D1. Polysome association experiments suggest that this is primarily caused by reduced translation initiation of the corresponding psbA mRNA. Comparison of mRNA steady state levels indicated that the psbA mRNA is significantly reduced in hcf173. Furthermore, the determination of the psbA mRNA half-life revealed an impaired RNA stability. The HCF173 gene was identified by map-based cloning, and its identity was confirmed by complementation of the hcf phenotype. HCF173 encodes a protein with weak similarities to the superfamily of the short-chain dehydrogenases/reductases. The protein HCF173 is localized in the chloroplast, where it is mainly associated with the membrane system and is part of a higher molecular weight complex. Affinity chromatography of an HCF173 fusion protein uncovered the psbA mRNA as a component of this complex.
authors: Institut für Entwicklungs und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität, 40225 Düsseldorf, Germany.
authors: Kerstin
authors: K
authors: Schult
authors: Karin
authors: K
authors: Meierhoff
authors: Susanne
authors: S
authors: Paradies
authors: Thomas
authors: T
authors: Töller
authors: Petra
authors: P
authors: Wolff
authors: Peter
authors: P
authors: Westhoff
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D001483:Base Sequence; D002467:Cell Nucleus / Q000502:physiology*; D002846:Chromatography, Affinity; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D003609:Dactinomycin / Q000494:pharmacology; D039642:Eukaryotic Initiation Factors / Q000235:genetics / Q000378:metabolism*; D015151:Immunoblotting; D008969:Molecular Sequence Data; D016296:Mutagenesis; D045332:Photosystem II Protein Complex / Q000235:genetics; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D018744:DNA, Plant; D039642:Eukaryotic Initiation Factors; C523214:HCF173 protein, Arabidopsis; D045332:Photosystem II Protein Complex; D012333:RNA, Messenger; D018749:RNA, Plant; D003609:Dactinomycin