[Experiment study on the diagnostic value of bile K-ras gene mutation responsible to the hepatic metastasis of pancreatic cancer].
OBJECTIVE
To investigate the early diagnosis value of bile K-ras mutation responsible for hepatic metastasis of pancreatic cancer.


METHODS
While building the animal models with pancreatic cancer and hepatic metastasis of pancreatic cancer of rats, we collected bile from the model animals, and used the polymerase chain reaction and restriction fragment-length polymorphism (PCR-RFLP) to evaluate the point mutation at exon codon 12 of K-ras gene in bile. Then, the above result was compared with pathological slice.


RESULTS
The positive rates of K-ras point mutation in bile were 84.2% (16/19) for the model with hepatic metastasis of pancreatic cancer, and 0.0% (0/16) for the model with pancreatic cancer without hepatic metastasis. For the model with hepatic metastasis of pancreatic cancer, the sensitivity, specificity, positive prediction or negative prediction value of surveying K-ras mutation in bile was 84.2%, 85.7%, 84.2% or 85.7% respectively.


CONCLUSIONS
Both the mutability and the specificity of K-ras gene mutation at codon 12 in bile are high. Our above work can supply a new elemental filter method for the early diagnosis of hepatic metastasis of pancreatic cancer.
Zhou
Xiang
X
Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Zhang
Zhao-Da
ZD
Tian
Bo-Le
BL
Han
Fang-Hai
FH
Hu
Wei-Ming
WM
Jia
Wei
W
Lu
Hui-Ming
HM
D000818:Animals; D001646:Bile / Q000378:metabolism*; D045744:Cell Line, Tumor; D003062:Codon / Q000235:genetics; D005091:Exons / Q000235:genetics; D005260:Female; D011905:Genes, ras / Q000235:genetics*; D008113:Liver Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000556:secondary*; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology*; D017354:Point Mutation; D051381:Rats; D017207:Rats, Sprague-Dawley
D003062:Codon