The chloroxoquinolinic derivative 6-chloro-1,4-dihydro-4-oxo-1-(beta-D-ribofuranosyl) quinoline-3-carboxylic acid inhibits HSV-1 adsorption by impairing its adsorption on HVEM.
In this paper, we describe that the oxoquinolinic acid derivative (compound A) inhibited HSV-1 adsorption on Vero cells. This effect was achieved with an EC(50) value of 10 +/- 2.0 microM and with low cytotoxicity, since the CC(50) value for compound A was >1000 microM. Moreover, we demonstrate for the first time that adsorption inhibition was due to the blockage of the interactions between HSV-1 and the cellular receptor herpes virus entry mediator (HVEM). These results show that compound A can prevent HSV-1 infection in Vero cells, encouraging further studies to determine at what level compound A inhibits HSV-1-HVEM interactions.
Souza
T M L
TM
Laboratório de Virologia Molecular, Departamento de Biologia Celular e Molecular, Instituto de Biologia, Universidade Federal Fluminense, Niterói, RJ, Brazil.
De Souza
M C Bastos V
MC
Ferreira
V F
VF
Canuto
C V B Santos
CV
Marques
I Pereira
IP
Fontes
C F L
CF
Frugulhetti
I C P P
IC
D000212:Acyclovir / Q000494:pharmacology; D000327:Adsorption; D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology; D016466:CHO Cells; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003412:Cricetulus; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000472:pathogenicity; D066298:In Vitro Techniques; D015363:Quinolones / Q000737:chemistry / Q000494:pharmacology*; D053240:Receptors, Tumor Necrosis Factor, Member 14 / Q000187:drug effects* / Q000502:physiology; D014709:Vero Cells
D000998:Antiviral Agents; D015363:Quinolones; D053240:Receptors, Tumor Necrosis Factor, Member 14; D000212:Acyclovir