title: Multi-grams scale purification of xanthanolides from Xanthium macrocarpum. Centrifugal partition chromatography versus silica gel chromatography.
abstract: Plants of the Asteraceae family are known as a source of sesquiterpene lactones with interesting biological activities. The purification of several xanthanolides (xanthathin, 4-epi-xanthanol and 4-epi-isoxanthanol) was realized in one step, directly from the crude chloroformic extract of the leaves of X. macrocarpum by silica gel chromatography and for the first time by liquid/liquid chromatography (counter-current chromatography/centrifugal partition chromatography), using a FCPC 5L (Fast centrifugal partition chromatograph, Kromaton Technologies, Angers, France). Recovery, purity of xanthathin and solvent consumption were improved with the liquid/liquid chromatography compared to solid/liquid chromatography.
authors: Laboratoire SONAS EA 921, UFR des Sciences Pharmaceutiques et Ingénierie de la Santé, 16 Bd Daviers, 49100 Angers, France.
authors: Benoit
authors: B
authors: Pinel
authors: Grégoire
authors: G
authors: Audo
authors: Sabine
authors: S
authors: Mallet
authors: Marie
authors: M
authors: Lavault
authors: François
authors: F
authors: De La Poype
authors: Denis
authors: D
authors: Séraphin
authors: Pascal
authors: P
authors: Richomme
mesh_terms: D002498:Centrifugation / Q000379:methods*; D002850:Chromatography, Gel / Q000379:methods*; D005663:Furans / Q000032:analysis / Q000737:chemistry*; D015394:Molecular Structure; D015203:Reproducibility of Results; D031210:Xanthium / Q000737:chemistry*
chemical_list: D005663:Furans; C022186:xanthatin