Decontamination of heart valve and arterial allografts in the European Homograft Bank (EHB): comparison of two different antibiotic cocktails in low temperature conditions.
The aim of the study was to compare the efficiency of two different antibiotic cocktails in the cardiovascular allograft decontamination. Low temperature, low-concentration antibiotic cocktail with Cefoxitin, Lincomycin, Polymixin B and Vancomycin was decontamination protocol in EHB for many years. The modified cocktail doesn't contain Cefoxitin. The study had two steps. First step: cardiovascular allografts from 80 donors are incubated in classical (group 1) or modified cocktail (group 2). Second step: 184 and 182 allografts of group 1 and group 2 are incubated in the modified and classical antibiotic cocktail, respectively. The bacteriological examination is performed in three steps: A-transport solution, B-decontamination solution and C-cryopreservation solution. During the first step 23.75% of the tissues were initially contaminated mainly with Staphylococcus (78.95%). 93.75% of the allografts of group 1 and 100% of group 2 were sterile after incubation (p = 0.058). 25.54% and 30.77% of group 1 and 2, respectively were contaminated in A-examination during the second step. Staphylococci were isolated in 82.98% and 69.64% in group 1 and 2, respectively. About 4.35% of group 1 and 5.5% of group 2 were contaminated in A, B, and C whereas 5.4% of group 1 and 4.4% of group 2 were contaminated in B or C after being sterile in A. Finally 9.78% of the tissues were rejected and 90.22% cryopreserved in the modified, whereas 9.89% rejected and 90.11% accepted in the classical group (p = 0.1). The difference was non-significant in the level of decontamination between the two cocktails. Contamination of some tissues with low growing, low-pathogen germs that appeared in B or C examination, couldn't be explained. This issue needs complementary investigation.
Jashari
R
R
European Homograft Bank, International Association, c/o Military Hospital, Rue Bruynstaat, Brussels, 1120, Belgium. jasharir@yahoo.com
Tabaku
M
M
Van Hoeck
B
B
Cochéz
C
C
Callant
M
M
Vanderkelen
A
A
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001158:Arteries / Q000382:microbiology / Q000637:transplantation*; D015925:Cryopreservation; D003666:Decontamination; D005060:Europe; D006351:Heart Valves / Q000382:microbiology / Q000637:transplantation*; D006801:Humans; D011446:Prospective Studies; D014015:Tissue Banks; D014184:Transplantation, Homologous
D000900:Anti-Bacterial Agents