title: Arabidopsis rd29A::DREB1A enhances freezing tolerance in transgenic potato.
abstract: The freezing tolerance of 38 independent transgenic potato lines derived from the cultivar Desiree was tested in vitro using plantlets. The lines were transgenic for the DREB1A gene under control of the rd29A promoter, both of which were derived from Arabidopsis thaliana. The level of damage caused by freezing varied significantly among the transgenic clones and a non-transgenic control (cv. Desiree). Phenotypic evaluation indicated that the variable responses to freezing were attributable to genotypic variation, but freezing tolerance was not dependent on the number of insertions. Northern blot analysis using a DREB1A cDNA probe revealed high levels of DREB1A expression among the transgenic clones during the initial cold exposure at 4 degrees C (after 2 h) and in the early stages of freezing (-20 degrees C, 1-10 min). Furthermore, a linear correlation was detected between the level of expression and the phenotypic response for all lines except D138. Thus, in the case of potato, a significant increase in freezing tolerance was observed in vitro on a small scale following the introduction of rd29A::DREB1A. Additional testing will show whether this strategy can be used for tolerance breeding in potato and to increase the freezing tolerance of other agriculturally important crops.
authors: Gene Research Center, Graduate School of Life and Environmental Sciences, University of Tsukuba, Ten-nodai 1-1-1, Ibaraki, Tsukuba 305-8752, Japan.
authors: Babak
authors: B
authors: Behnam
authors: Akira
authors: A
authors: Kikuchi
authors: Fevziye
authors: F
authors: Celebi-Toprak
authors: Mie
authors: M
authors: Kasuga
authors: Kazuko
authors: K
authors: Yamaguchi-Shinozaki
authors: Kazuo N
authors: KN
authors: Watanabe
mesh_terms: D000222:Adaptation, Physiological; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D005615:Freezing; D018506:Gene Expression Regulation, Plant; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D011198:Solanum tuberosum / Q000235:genetics* / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; C114578:DREB1A protein, Arabidopsis; C520629:RD29a protein, Arabidopsis; D014157:Transcription Factors