title: Characterization of photosynthetically active duckweed (Wolffia australiana) in vitro culture by Respiration Activity Monitoring System (RAMOS).
abstract: The feasibility of oxygen transfer rate (OTR) measurement to non-destructively monitor plant propagation and vitality of photosynthetically active plant in vitro culture of duckweed (Wolffia australiana, Lemnaceae) was tested using Respiration Activity Monitoring System (RAMOS). As a result, OTR proofed to be a sensitive indicator for plant vitality. The culture characterization under day/night light conditions, however, revealed a complex interaction between oxygen production and consumption, rendering OTR measurement an unsuitable tool to track plant propagation. However, RAMOS was found to be a useful tool in preliminary studies for process development of photosynthetically active plant in vitro cultures.
authors: Division Juelich, Universtity of Applied Science Aachen, Ginsterweg 1, Juelich, Germany.
authors: Henrik
authors: H
authors: Rechmann
authors: Andrea
authors: A
authors: Friedrich
authors: Dara
authors: D
authors: Forouzan
authors: Stefan
authors: S
authors: Barth
authors: Heide
authors: H
authors: Schnabl
authors: Manfred
authors: M
authors: Biselli
authors: Robert
authors: R
authors: Boehm
mesh_terms: D029064:Araceae / Q000254:growth & development* / Q000378:metabolism* / Q000528:radiation effects; D008027:Light; D010100:Oxygen / Q000378:metabolism; D010101:Oxygen Consumption; D046509:Tissue Culture Techniques / Q000295:instrumentation* / Q000379:methods
chemical_list: D010100:Oxygen