Topological and chronological features of the impairment of glucose metabolism induced by 1-methyl-4-phenylpyridinium ion (MPP+) in rat brain slices.
1-Methyl-4-phenylpyridinium (MPP(+)) was added directly to fresh rat brain slices and the dynamic changes in the cerebral glucose metabolic rate (CMRglc) were serially and two-dimensionally measured with [(18)F]2-fluoro-2-deoxy-D-glucose as a tracer. MPP(+) dose-dependently increased CMRglc, reflecting enhanced glycolysis compensating for the decrease in aerobic metabolism. While the CMRglc enhancement induced by MPP(+) (<10 microM) was restricted to the striatum, MPP(+) (>or=10 microM) induced a significant CMRglc enhancement in all brain regions. MPP(+) at high concentration (1 mM) eventually initiated rapid metabolic collapse, with failure to sustain anaerobic glycolysis.
Maruoka
N
N
Department of Neuropsychiatry, University of Fukui, Fukui, Japan.
Murata
T
T
Omata
N
N
Takashima
Y
Y
Fujibayashi
Y
Y
Wada
Y
Y
D015655:1-Methyl-4-phenylpyridinium / Q000633:toxicity*; D000693:Anaerobiosis / Q000187:drug effects / Q000502:physiology; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism* / Q000502:physiology; D001931:Brain Mapping / Q000379:methods; D005947:Glucose / Q000172:deficiency / Q000378:metabolism*; D006019:Glycolysis / Q000187:drug effects / Q000502:physiology; D006540:Herbicides / Q000633:toxicity*; D008297:Male; D009924:Organ Culture Techniques; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D006540:Herbicides; D005947:Glucose; D015655:1-Methyl-4-phenylpyridinium