title: Optimization of gelatine extraction from grass carp (Catenopharyngodon idella) fish skin by response surface methodology.
abstract: To establish the optimum gelatine extraction conditions from grass carp fish skin, response surface methodology (RSM) was adopted in this study. The effects of concentration of HCl (%, A), pre-treatment time (h, B), extraction temperature ( degrees C, C) and extraction time (h, D) were studied. The responses were yield (%) and gel strength (g). A=1.19%, B=24 h, C=52.61 degrees C and D=5.12h were determined as the optimum conditions while the predicted responses were 19.83% yield and 267 g gel strength. Gelling and melting points were 19.5 degrees C and 26.8 degrees C, respectively. Moreover, grass carp gelatine showed high contents of imino acids (proline and hydroxyproline) 19.47%. RSM provided a powerful tool to optimize the extraction parameters and the results may be adapted for industrial extraction of gelatine from grass carp fish skins.
authors: Southern Yangtze University, School of Food Science, Research Centre of Functional Food Engineering, Ministry of Education, Wuxi, PR China.
authors: Ladislaus M
authors: LM
authors: Kasankala
authors: Yan
authors: Y
authors: Xue
authors: Yao
authors: Y
authors: Weilong
authors: Sun D
authors: SD
authors: Hong
authors: Qian
authors: Q
authors: He
mesh_terms: D000818:Animals; D002347:Carps / Q000033:anatomy & histology / Q000378:metabolism*; D005780:Gelatin / Q000302:isolation & purification* / Q000378:metabolism; D012867:Skin / Q000737:chemistry* / Q000378:metabolism*; D013696:Temperature
chemical_list: D005780:Gelatin