title: Protective immunity conferred by recombinant Pasteurella multocida lipoprotein E (PlpE).
abstract: The genes encoding Pasteurella multocida lipoprotein E (PlpE) and lipoprotein B (PlpB) were cloned from P. multocida strain X-73 (serotype A:1) and expressed in Escherichia coli. The protective immunity conferred by recombinant PlpE (r-PlpE) and PlpB (r-PlpB) on mice and chickens was evaluated. The results showed that mice immunized with 10microg of purified r-PlpE were protected (80-100% survival rate) against challenge infection with 10 or 20 LD(50) of P. multocida strains X-73 (serotype A:1), P-1059 (serotype A:3) and P-1662 (serotype A:4). In contrast, mice immunized with r-PlpB were not protected. Chickens immunized with 100microg of purified r-PlpE were protected (63-100% survival rate) against lethal challenge infection with strains X-73 and P-1662, whereas those immunized with r-PlpB were not. Sequence analyses showed that PlpE from different strains of P. multocida exhibited 90.8-100% sequence identity to each other, suggesting that PlpE might serve as a cross-protective antigen. This is the first report of a recombinant P. multocida antigen that confers cross protection on animals.
authors: Graduate Institute of Veterinary Microbiology, National Chung Hsing University, Taichung 402, Taiwan.
authors: Jin-Ru
authors: JR
authors: Wu
authors: Jui-Hung
authors: JH
authors: Shien
authors: Happy K
authors: HK
authors: Shieh
authors: Chih-Feng
authors: CF
authors: Chen
authors: Poa-Chun
authors: PC
authors: Chang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001055:Apolipoproteins B / Q000235:genetics / Q000276:immunology; D001057:Apolipoproteins E / Q000235:genetics / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D001428:Bacterial Vaccines / Q000276:immunology*; D002645:Chickens; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D008074:Lipoproteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010326:Pasteurella Infections / Q000276:immunology* / Q000517:prevention & control; D016979:Pasteurella multocida / Q000276:immunology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013047:Specific Pathogen-Free Organisms; D016019:Survival Analysis; D022223:Vaccines, Subunit / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification
chemical_list: D001055:Apolipoproteins B; D001057:Apolipoproteins E; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D004269:DNA, Bacterial; D008074:Lipoproteins; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic