title: [Protein-protein interaction between hypothetical protein Rv1246c and Rv1247c of Mycobacterium tuberculosis].
abstract: OBJECTIVE
  To investigate the protein-protein interaction between hypothetical protein Rv1246c and Rv1247c of Mycobacterium tuberculosis.
  
  
  METHODS
  By PCR technique, the complete open-reading frame sequences of Rv1246c and Rv1247c gene were amplified from the M. tuberculosis H37Rv genomic DNA as template. The PCR-amplified cDNAs of Rv1247c and Rv1246c gene were subcloned into pGBKT7 and pGADT7-Rec vector respectively for constructing recombinant plasmids pGBKT7-Rv1247c and pGADT7-Rv1246c. After verified by restriction endonuclease digestion and DNA sequence determination, the recombinant vectors were used to transform the yeast cell AH109 by lithium acetate method.
  
  
  RESULTS
  The yeast cells co-transformed with pGBKT7-Rv1247c and pGADT7-Rv1246c grew on SD/-Ade/-His/-Leu/-Trp plates, and the beta-galactosidase activity assays showed the positive signal.
  
  
  CONCLUSIONS
  The hypothetical protein Rv1246c and Rv1247c could interact with each other in yeast cells.
authors: Department of Infection and Immunity, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors: Zheng-Ling
authors: ZL
authors: Shang
authors: Lang
authors: L
authors: Bao
authors: Su-Xia
authors: SX
authors: Yao
authors: Hui-Dong
authors: HD
authors: Zhang
authors: Yan
authors: Y
authors: Li
authors: Qi
authors: Q
authors: Zhong
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D005822:Genetic Vectors / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D020798:Two-Hybrid System Techniques; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D001616:beta-Galactosidase