The influence of staphylococcin T (StT) on the mouse fibroblasts (3T3) viability, an in vitro experiment.
Staphylococcin T (StT) is a bacteriocin, which can serve as an antibiotic. The influence of StT on the mammalian cells viability should be examined before its possible applications. The aim of this work was to study the influence of StT on mouse fibroblasts viability in vitro. StT was delivered as a deposit in agar. 3T3 fibroblasts were used in viability tests. Cells were incubated with StT for 24 and 48 h. In the first controls, cells grew without the agar block, in the second controls, the agar block contained no StT. Trypan blue exclusion test was performed. Growth inhibition of sensitive strain SAU-209P was observed after 24 h incubation with cell culture medium containing StT. Antimicrobial activity of StT in culture medium was confirmed even after 2 months. The viability of 3T3 cells in the second control group was 50% lower than in the first and 25% higher than in StT treated group during the first day. It was noticed mechanical damage of growing cells caused by agar blocks. The number of cells cultured with StT was similar to that of second control group after 2 days. There were no changes in morphology of cells in all groups. It follows that StT had unimportant influence on the fibroblasts (3T3) viability in culture. StT may be considered for future animal trails.
Drewa
Tomasz
T
Department of Tissue Engineering, Chair of Medical Biology, N. Copernicus University, Bydgoszcz, Poland. tomaszdrewa@wp.pl
Krawczyk
Agnieszka
A
Gospodarek
Eugenia
E
Jachna-Sawicka
Katarzyna
K
Bugalski
Roman
R
Kierzenkowska-Mila
Celestyna
C
D016475:3T3 Cells; D000818:Animals; D001430:Bacteriocins / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D051379:Mice; D010455:Peptides / Q000494:pharmacology*
D001430:Bacteriocins; D010455:Peptides; C057656:gallidermin