Issues concerning the large scale cryopreservation of peripheral blood mononuclear cells (PBMC) for immunotherapy trials.
Immunotherapy of cancer is being developed as an alternative or adjuvant to conventional therapies such as: surgery, chemotherapy and/or radiation treatment. Immunotherapy laboratories routinely process and prepare for injection large numbers of anti-tumor effector cells. The process of cryopreservation is critical to the success of immunotherapy. Standardized safe procedures are required. In the current report, we show the ability to cryopreserve peripheral blood mononuclear cells (PBMC) in Plasmalyte-A, a fluid replacement medium approved by the FDA. These studies show that this medium can be used in place of human serum in terms of cell recovery, cell surface phenotype and response to PHA. However, T cell cytokine release stimulated through the CD3 receptor was altered following the cryopreservation process. These results are important toward the improvement of cryopreservation techniques for their use in immunotherapy.
Best
Angela
A
Department of Microbiology/Immunology and Molecular Genetics and the Markey Cancer Center, College of Medicine, University of Kentucky, Room 312 Combs Building, 800 Rose Street, Lexington, KY 40536-0093, USA.
Hidalgo
Giovi
G
Mitchell
Katherine
K
Yannelli
John R
JR
D017252:CD3 Complex / Q000032:analysis; D002986:Clinical Trials as Topic / Q000379:methods; D015925:Cryopreservation / Q000379:methods*; D003470:Culture Media / Q000494:pharmacology; D003713:Dendritic Cells / Q000187:drug effects / Q000502:physiology*; D004573:Electrolytes / Q000494:pharmacology; D006801:Humans; D007167:Immunotherapy / Q000379:methods*; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000502:physiology*; D010952:Plasma Substitutes / Q000494:pharmacology; D015203:Reproducibility of Results; D013601:T-Lymphocytes / Q000502:physiology
D017252:CD3 Complex; D003470:Culture Media; D004573:Electrolytes; D010952:Plasma Substitutes; D016753:Interleukin-10; D007371:Interferon-gamma; C048013:Plasmalyte A