A novel universal linker for efficient synthesis of phosphorothioate oligonucleotides.
A versatile and conformationally preorganized universal linker molecule is reported here for efficient synthesis of phosphorothioate oligonucleotides. With respect to nucleoside loaded support, comparable yield and quality based on ion-pair LC-MS are obtained for both deoxy and 2'-O-methoxyethyl modified phosphorothioate oligonucleotides. No 3'-phosphate or phosphorothioate monoester or any modification of universal molecule still attached to oligonucleotide was observed. [structure: see text]
Wang
Zhiwei
Z
Isis Pharmaceuticals, Carlsbad, California 92008. USA.
Olsen
Phil
P
Ravikumar
Vasulinga T
VT
D002621:Chemistry / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000379:methods; D007477:Ions; D013058:Mass Spectrometry / Q000379:methods; D008956:Models, Chemical; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D016376:Oligonucleotides, Antisense / Q000737:chemistry; D010758:Phosphorus / Q000737:chemistry; D013873:Thionucleotides / Q000138:chemical synthesis* / Q000737:chemistry
D007477:Ions; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; D013873:Thionucleotides; D010758:Phosphorus