Photo cross-linkable Biodendrimers as ophthalmic adhesives for central lacerations and penetrating keratoplasties.
OBJECTIVE
Biodendrimer-based hydrogel adhesives were derived from biocompatible building blocks and poly(ethylene glycol) of 3,400, 10,000 and 20,000 g/mole. The leaking pressures were determined for these adhesives when used to seal 4.1-mm central lacerations and penetrating keratoplasties (PKPs) in enucleated porcine eyes.


METHODS
Three biodendrimers, ([G1]-PGLSA-MA)(2)-PEG(3,400), ([G1]-PGLSA-MA)(2)-PEG(10,000), and ([G1]-PGLSA-MA)(2)-PEG(20,000), at a range of weight percents were each photo cross-linked in the presence of a photo-initiator to form a hydrated network. These biodendrimer-based adhesives were applied directly to a 4.1-mm linear central laceration. In a PKP, the corneal button was initially secured with 8 or 16 sutures and then sealed with the adhesive.


RESULTS
For the 4.1-mm central lacerations, the ([G1]-PGLSA-MA)(2)-PEG(3,400) at 20% and 40% wt/vol, the ([G1]-PGLSA-MA)(2)-PEG(10,000) at 10 and 20% wt/vol, and the ([G1]-PGLSA-MA)(2)-PEG(20,000) at 20% wt/vol held to leaking pressures above 200 mm Hg. In the autograft with 16 sutures, the 20% wt/vol of the ([G1]-PGLSA-MA)(2)-PEG(3,400), ([G1]-PGLSA-MA)(2)-PEG(10,000), and ([G1]-PGLSA-MA)(2)-PEG(20,000) held to a pressure at or above 100 mm Hg. In the autograft with eight sutures, the ([G1]-PGLSA-MA)(2)-PEG(10,000) and ([G1]-PGLSA-MA)(2)-PEG(20,000) formulations at 20% wt/vol held to leaking pressures of 85 +/- 22 and 80 +/- 30 mm Hg, respectively.


CONCLUSIONS
The 10% wt/vol ([G1]-PGLSA-MA)(2)-PEG(10,000) formulation withheld leaking pressures above 200 mm Hg when used to secure a 4.1 mm central laceration. The 20% wt/vol ([G1]-PGLSA-MA)(2)-PEG(10,000) and ([G1]-PGLSA-MA)(2)-PEG(20,000) formulations, with 8 or 16 sutures, secured the PKP well above normal IOP. Biodendrimer-based adhesives are of potential use for repairing corneal wounds.
Degoricija
Lovorka
L
Departments of Chemistry, Boston University, Boston, Massachusetts, USA.
Johnson
C Starck
CS
Wathier
Michel
M
Kim
Terry
T
Grinstaff
Mark W
MW
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000627:therapeutic use*; D003315:Cornea / Q000473:pathology; D065306:Corneal Injuries; D003432:Cross-Linking Reagents; D050091:Dendrimers / Q000627:therapeutic use*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D007429:Intraocular Pressure; D015948:Keratoplasty, Penetrating; D022125:Lacerations / Q000188:drug therapy* / Q000473:pathology; D008027:Light; D011092:Polyethylene Glycols / Q000737:chemistry / Q000627:therapeutic use*; D013529:Surgical Wound Dehiscence; D013552:Swine; D013718:Tensile Strength; D014014:Tissue Adhesives / Q000737:chemistry / Q000627:therapeutic use*; D014945:Wound Healing / Q000187:drug effects*
C520269:((G1)-PGLSA-MA)2-PEG; D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D050091:Dendrimers; D014014:Tissue Adhesives; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols