title: [Determination of matrine, sophoridine and oxymatrine in Compound Kushen Injection by HPLC].
abstract: OBJECTIVE
  To establish a high performance liquid chromatography (HPLC) method for determination of matrine, sophoridine and oxymatrine in Compound Kushen Injection.
  
  
  METHODS
  Alltima amino column was used with acetonitrile-3% phosphoric acid-ethyl alcohol (80:10:10) as the mobile phase and the detection wavelength was at 220 nm.
  
  
  RESULTS
  The mean recovery of matrine, sophoridine and oxymatrine in Compound Kushen Injection was 99.51% (RSD 1. 58%), 99.24% (RSD 1.44%) and 100.22% (RSD 1.85%), respectively.
  
  
  CONCLUSIONS
  The method was simple, rapid, accurate and specific and suitable for quality control of the product.
authors: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors: Juan
authors: J
authors: Tian
authors: Wei-hao
authors: WH
authors: Wang
authors: Hui-min
authors: HM
authors: Gao
authors: Zhi-min
authors: ZM
authors: Wang
mesh_terms: D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D011786:Quality Control; D011807:Quinolizines / Q000032:analysis*; D015203:Reproducibility of Results; D000093842:Matrines
chemical_list: D000470:Alkaloids; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D011807:Quinolizines; C037573:oxymatrine; D000093842:Matrines