title: Relationship between Kir2.1/Kir2.3 activity and their distributions between cholesterol-rich and cholesterol-poor membrane domains.
abstract: Our earlier studies have shown that Kir2.x channels are suppressed by an increase in the level of cellular cholesterol, whereas cholesterol depletion enhances the activity of the channels. In this study, we show that Kir2.1 and Kir2.3 channels have double-peak distributions between cholesterol-rich (raft) and cholesterol-poor (non-raft) membrane fractions, indicating that the channels exist in two different types of lipid environment. We also show that whereas methyl-beta-cyclodextrin-induced cholesterol depletion removes cholesterol from both raft and non-raft membrane fractions, cholesterol enrichment results in cholesterol increase exclusively in the raft fractions. Kinetics of both depletion-induced Kir2.1 enhancement and enrichment-induced Kir2.1 suppression correlate with the changes in the level of raft cholesterol. Furthermore, we show not only that cholesterol depletion shifts the distribution of the channels from cholesterol-rich to cholesterol-poor membrane fractions but also that cholesterol enrichment has the opposite effect. These observations suggest that change in the level of raft cholesterol alone is sufficient to suppress Kir2 activity and to facilitate partitioning of the channels to cholesterol-rich domains. Therefore, we suggest that partitioning to membrane rafts plays an important role in the sensitivity of Kir2 channels to cholesterol.
authors: Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, USA.
authors: Saloni
authors: S
authors: Tikku
authors: Yulia
authors: Y
authors: Epshtein
authors: Heidi
authors: H
authors: Collins
authors: Alexander J
authors: AJ
authors: Travis
authors: George H
authors: GH
authors: Rothblat
authors: Irena
authors: I
authors: Levitan
mesh_terms: D000818:Animals; D016466:CHO Cells; D002784:Cholesterol / Q000172:deficiency / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D015640:Ion Channel Gating; D007700:Kinetics; D021962:Membrane Microdomains / Q000378:metabolism*; D008564:Membrane Potentials; D024661:Potassium Channels, Inwardly Rectifying / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D014162:Transfection; D047392:beta-Cyclodextrins / Q000378:metabolism
chemical_list: C489461:Kir2.1 channel; D024661:Potassium Channels, Inwardly Rectifying; D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin; D002784:Cholesterol