title: Imaging of silkworm meiotic chromosome by atomic force microscopy.
abstract: Although structural information of mitotic chromosomes has been accumulated, little information is available for meiotic chromosome structures. Here, we applied atomic force microscopy (AFM) to investigate the ultrastructures of the silkworm, Bombyx mori, meiotic pachytene chromosome in its native state with nanometer scale resolution. Two levels of DNA folding were observed on the meiotic chromosome surface, 50-70 nm granules, which were considered to be 30 nm chromatin fibers, and spherical protrusions of 400-600 nm, which were considered to be chromomeres and arranged on the surface of the chromosome parallel to the chromosome longitudinal axis. These observations suggested that AFM study is an excellent approach for obtaining information concerning the silkworm pachytene chromosome higher order structure.
authors: Insect Genome Research Unit, Division of Insect Sciences, National Institute of Agrobiological Science, Tsukuba, Ibaraki, Japan.
authors: J
authors: J
authors: Narukawa
authors: K
authors: K
authors: Yamamoto
authors: T
authors: T
authors: Ohtani
authors: S
authors: S
authors: Sugiyama
mesh_terms: D000818:Animals; D012831:Bombyx / Q000235:genetics*; D002875:Chromosomes / Q000648:ultrastructure*; D018404:Isochromosomes; D018625:Microscopy, Atomic Force