Molecular and phylogenetic analysis of human influenza virus among Iranian patients in Shiraz, Iran.
Influenza is a viral respiratory pathogen responsible for frequent seasonal epidemics. There are currently three major human influenza viruses in global circulation namely A/H1N1, A/H3N2, and B. The objective of this study was to determine the human influenza virus genotypes in Shiraz, the capital of the Fars province of Iran. Three hundred patients suspected with human influenza virus infection were enrolled in this survey (2004-2005). The throat samples were cultured and titrated by hemagglutination (HA) assay. Typing and subtyping were performed by an in-house developed multiplex RT-PCR. Moreover, the phylogenetic analysis was carried out for HA gene. A total of 24 samples were found to be positive for human influenza virus infection, 17 H1N1 and 7 H3N2. These results were in agreement with the HI assay. The phylogenetic analysis results revealed that the Iranian H1N1 isolated were close to the A/New Caledonia/20/99 vaccine strain genetically and the Iranian H3N2 isolates were also related closely to the Fujian/411/021 and California/7/2004 vaccine strains. However, a slight genetic drift was found in these isolates. In conclusion, it was demonstrated that both influenza A subtypes A/H1N1 and A/H3N2 were dominant among Iranian patients in Shiraz during the 2004/5 winter season.
Shahidi
Mahsa
M
Influenza Unit, Pasteur Institute of Iran, Tehran, Iran.
Kheiri
Masoumeh Tavassoti
MT
Amini-Bavil-Olyaee
Samad
S
Hosseini
Masoud
M
Moattari
Afagh
A
Tabatabaeian
Mansoureh
M
Bashar
Rouzbeh
R
Sarrami-Forooshani
Ramin
R
Mahboudi
Fereidoun
F
D000293:Adolescent; D000328:Adult; D002648:Child; D005838:Genotype; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics*; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D053122:Influenza A Virus, H3N2 Subtype / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D007251:Influenza, Human / Q000821:virology*; D029524:Betainfluenzavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D007492:Iran; D008875:Middle Aged; D008969:Molecular Sequence Data; D009975:Orthomyxoviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010614:Pharynx / Q000821:virology; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020539:Sequence Analysis, Protein
D019267:Hemagglutinin Glycoproteins, Influenza Virus