Affinity chromatography with immobilized DNA stationary phase for biological fingerprinting analysis of traditional Chinese medicines.
A stationary phase for high performance affinity chromatography with immobilization of DNA onto silica gel was prepared and characterized. The effect of the ionic strength, concentration of Mg2+, EDTA and CH3CN in the mobile phase on the retention of alkaloids were investigated. With this stationary phase, biological fingerprinting analysis of traditional Chinese medicines (TCMs) Coptis chinensis Franch and Rheum palmatum L. was performed with both one-dimensional (1-D) and two-dimensional (2-D) chromatography. The 1-D chromatography was performed with isocratic and gradient elution and 2-D chromatography was developed with immobilized DNA column combined with silica monolithic ODS column. It was found that 7 compounds in Coptis chinensis Franch including berberine, palmatine and jatrorrhizine, 14 compounds in Rheum palmatum L. including aloe-emodin, rhein, emodin, chrysophannol-8-O-glucophranoside and physionl-8-O-glucophranoside were active in binding to the immobilized DNA.
Su
Xingye
X
National Chromatographic R.&A. Centre, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Hu
Lianghai
L
Kong
Liang
L
Lei
Xiaoyuan
X
Zou
Hanfa
H
D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004247:DNA / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000302:isolation & purification; D013058:Mass Spectrometry / Q000379:methods
D004365:Drugs, Chinese Herbal; D004247:DNA; C427203:calf thymus DNA