title: Determination of sertraline and N-desmethylsertraline in human plasma by CE with LIF detection.
abstract: A method has been developed for the analysis of the antidepressant drug sertraline together with its main metabolite N-desmethylsertraline (DMS) in human plasma. It is based on CE with LIF detection (lambda = 488 nm). A SPE procedure is employed for biological sample pretreatment, followed by a derivatization step with FITC; reboxetine was the internal standard. The effect of CD, acetone and N-methyl-D-glucamine (GLC) as constituents of the BGE for analyte separation was investigated. The final BGE consisted of 20 mM carbonate buffer, pH 9.0, with 2.5 mM heptakis(2,6-di-O-methyl)-beta-CD, 50 mM GLC and 20% v/v acetone. With 30 kV applied voltage, the electrophoretic run is completed in 7.5 min. Linearity was observed in the plasma concentration range from 3.0 to 500 ng/mL for sertraline and 4.0 to 500 ng/mL for DMS. Extraction yield was >97.1%, precision - expressed as RSD% - was <3.7, accuracy (recovery) was >95.6%. Due to its sensitivity and selectivity, the method was suited for the analysis of plasma samples from patients undergoing therapy with sertraline.
authors: Department of Pharmaceutical Sciences, University of Bologna, Bologna, Italy.
authors: Alessandro
authors: A
authors: Musenga
authors: Ernst
authors: E
authors: Kenndler
authors: Laura
authors: L
authors: Mercolini
authors: Mario
authors: M
authors: Amore
authors: Salvatore
authors: S
authors: Fanali
authors: Maria Augusta
authors: MA
authors: Raggi
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020280:Sertraline / Q000031:analogs & derivatives* / Q000097:blood* / Q000302:isolation & purification
chemical_list: C065162:desmethylsertraline; D020280:Sertraline