title: Ultrastructural heterogeneity of gangliogliomas.
abstract: Gangliogliomas are rare brain tumors, composed of neuronal and glial cells mixed in a different proportion. The basic histopathological pattern of gangliogliomas is well recognized but the variable microscopic appearance still can pose a challenge to the neuropathologist. The authors reanalyzed their series of gangliogliomas in the files of two departments of neuropathology. All analyzed tumors fulfilled the WHO histological criteria of ganglioglioma. Seven tumors were examined by electron microscopy. The following ultrastructural features were graded: presence of dense-cored vesicles, synaptic vesicles, synapses and intermediate filaments, abundant basal membranes, dystrophic neurites, autophagic vacuoles, and multivesicular bodies. Most of the neoplastic neurons were large, polyglonal or oval with well-developed subcellular organelles, round nuclei, and prominent nucleoli. In most cases there were abundant dense core vesicles, observed in both the tumor cell bodies as well as in their processes. Synapses were typically observed. Intermediate filaments were abundant in all tumors. The most intriguing ultrastructural finding was abundant presence of autophagic vacuoles. In 4 cases, multivesicular bodies were observed. All of the tumors with multivesicular bodies also contained abundant autophagic vacuoles.
authors: Department of Molecular Pathology and Neuropathology, Medical University Lodz, Lodz, Poland.
authors: Beata
authors: B
authors: Sikorska
authors: Wielisław
authors: W
authors: Papierz
authors: Krzysztof
authors: K
authors: Zakrzewki
authors: Tomasz
authors: T
authors: Fiks
authors: Lech
authors: L
authors: Polis
authors: Pawel P
authors: PP
authors: Liberski
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001343:Autophagy; D001932:Brain Neoplasms / Q000503:physiopathology / Q000648:ultrastructure*; D002648:Child; D005260:Female; D018303:Ganglioglioma / Q000503:physiopathology / Q000648:ultrastructure*; D006801:Humans; D007150:Immunohistochemistry; D007382:Intermediate Filaments / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D016501:Neurites / Q000648:ultrastructure; D009457:Neuroglia / Q000648:ultrastructure*; D009474:Neurons / Q000648:ultrastructure*; D011044:Poland; D022142:Secretory Vesicles / Q000648:ultrastructure; D013569:Synapses / Q000648:ultrastructure; D013572:Synaptic Vesicles / Q000648:ultrastructure; D014617:Vacuoles / Q000648:ultrastructure