Protein profile of human lung squamous carcinoma cell line NCI-H226.
OBJECTIVE
To construct a database of human lung squamous carcinoma cell line NCI-H226 and to facilitate discovery of novel subtypes markers of lung cancer.


METHODS
Proteomic technique was used to analyze human lung squamous carcinoma cell line NCI-H226. The proteins of the NCI-H226 cells were separated by two-dimensional gel electrophoresis and identified by mass spectrometry.


RESULTS
The results showed that a good reproducibility of the 2-D gel pattern was attained. The position deviation of matched spots among three 2-D gels was 1.95 +/- 0.53 mm in the isoelectric focusing direction, and 1.73 +/- 0.45 mm in the sodium dodecyl sulfate-polyacrylamide gel electrophoresis direction. One hundred and twenty-seven proteins, including enzymes, signal transduction proteins, structure proteins, transport proteins, etc. were characterized, of which, 29 identified proteins in NCI-H226 cells were reported for the first time to be involved in lung cancer carcinogenesis.


CONCLUSIONS
The information obtained from this study could provide some valuable clues for further study on the carcinogenetic mechanism of different types of lung cancer, and may help us to discover some potential subtype-specific biomarkers of lung cancer.
Zhang
Hao
H
Key Laboratory of Cell Proliferation and Regulation of Ministry of Education, Universities' Confederated Institute of Proteomics, Beijing Normal University, Beijing, China.
Li
Na
N
Chen
Yue
Y
Huang
Ling-Yun
LY
Wang
Yi-Ching
YC
Fang
Gang
G
He
Da-Cheng
DC
Xiao
Xue-Yuan
XY
D014408:Biomarkers, Tumor / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000737:chemistry*; D045744:Cell Line, Tumor; D016208:Databases, Factual; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008175:Lung Neoplasms / Q000737:chemistry*; D009363:Neoplasm Proteins / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins