High expression of Tim-3 mRNA in urinary cells from kidney transplant recipients with acute rejection.
TIM-3 is a recently described molecule specifically expressed on Th1 differentiated T cells. We explored the usefulness of urinary mRNA profiles in the diagnosis of renal acute rejection (AR). Sixty urinary samples from renal transplant recipients simultaneously collected to allograft biopsy, (AR = 30 and No-AR =30), and 12 urinary samples from stable renal transplants were analyzed. Urinary mRNA encoding for TIM-3 and IFN-gamma were quantified using real time RT-PCR. TIM-3 mRNA was highly expressed in AR (559.19 +/- 644.41) compared to No-AR (3.78 +/- 7.20), and stable transplants (0.54 +/- 0.76), p < 0.001. To a lesser degree, IFN-gamma mRNA transcripts were also increased in AR (50.40 +/- 38.71), compared with No-AR (4.69 +/- 12.62), and stable transplants (0.38 +/- 0.44) p < 0.001. The highest expression of TIM-3 in AR makes it a promising noninvasive test for its diagnosis.
Renesto
P G
PG
Laboratory of Clinical and Experimental Immunology, Division of Nephrology, Universidade Federal de São Paulo, São Paulo, Brazil.
Ponciano
V C
VC
Cenedeze
M A
MA
Saraiva Câmara
N O
NO
Pacheco-Silva
A
A
D003430:Cross-Sectional Studies; D017931:DNA Primers; D015342:DNA Probes; D006084:Graft Rejection / Q000175:diagnosis* / Q000652:urine; D000072597:Hepatitis A Virus Cellular Receptor 2; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D016030:Kidney Transplantation / Q000276:immunology / Q000502:physiology*; D008565:Membrane Proteins; D012333:RNA, Messenger / Q000235:genetics*; D011991:Receptors, Virus / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013048:Specimen Handling
D017931:DNA Primers; D015342:DNA Probes; C448908:HAVCR2 protein, human; D000072597:Hepatitis A Virus Cellular Receptor 2; D007166:Immunosuppressive Agents; D008565:Membrane Proteins; D012333:RNA, Messenger; D011991:Receptors, Virus