title: Searchable high-resolution 2D gel proteome of the human colon crypt.
abstract: We seek alterations in protein patterns at the earliest possible step on the path to cancer, namely, in cells of the target tissue from normal persons versus the corresponding normally appearing cells from persons who are heterozygous for mutation in a tumor suppressor gene that predisposes strongly to carcinoma in that tissue. To begin a systematic comparison of the proteomes of cells from normal and from neoplastic colons, we have undertaken the isolation of human colon crypts that are derived from the normal-appearing mucosa of left (descending) colon of patients with sporadic colorectal cancer. Two-dimensional (2D) gel electrophoresis is a proteomic approach that excels in the resolution of protein isoforms. Here, we document the practicality of this approach with human samples using gels of three overlapping pH ranges. For the first time, about 800 nonredundant proteins and 900 isoforms from purified human colonic crypts were identified, permitting an assessment of the contributions of protein isoforms. These interactive, searchable, hyperlink-enabled proteome maps and gene ontology analyses will facilitate future studies to discover the earliest markers and intervention targets during progression to colon cancer.
authors: Division of Basic Science, Medical Science, and Population Science, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA.
authors: Bhavinkumar B
authors: BB
authors: Patel
authors: Xin-Ming
authors: XM
authors: Li
authors: Maketa P
authors: MP
authors: Dixon
authors: Elena L
authors: EL
authors: Blagoi
authors: Steven H
authors: SH
authors: Seeholzer
authors: Yibai
authors: Y
authors: Chen
authors: C Glenn
authors: CG
authors: Miller
authors: Yin A
authors: YA
authors: He
authors: Mazell
authors: M
authors: Tetruashvily
authors: Anam H
authors: AH
authors: Chaudhry
authors: Eileen
authors: E
authors: Ke
authors: Joan
authors: J
authors: Xie
authors: Harry
authors: H
authors: Cooper
authors: Alfonso
authors: A
authors: Bellacosa
authors: Margie L
authors: ML
authors: Clapper
authors: Bruce M
authors: BM
authors: Boman
authors: Tao
authors: T
authors: Zhang
authors: Samuel
authors: S
authors: Litwin
authors: Eric A
authors: EA
authors: Ross
authors: Peggy
authors: P
authors: Conrad
authors: James A
authors: JA
authors: Crowell
authors: Levy
authors: L
authors: Kopelovich
authors: Alfred
authors: A
authors: Knudson
authors: Anthony T
authors: AT
authors: Yeung
mesh_terms: D002374:Catalase / Q000032:analysis; D003106:Colon / Q000737:chemistry*; D015179:Colorectal Neoplasms / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000592:standards; D005982:Glutathione Transferase / Q000032:analysis; D006801:Humans; D011506:Proteins / Q000032:analysis* / Q000235:genetics; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D013482:Superoxide Dismutase / Q000032:analysis
chemical_list: D011506:Proteins; D020543:Proteome; D002374:Catalase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase