Antisense oligonucleotide targeting midkine suppresses in vivo angiogenesis.
OBJECTIVE
To evaluate the effect of antisense oligonucleotide targeting midkine (MK-AS) on angiogenesis in chick chorioallantoic membrane (CAM) and in situ human hepatocellular carcinoma (HCC).


METHODS
An in situ human hepatocellular carcinoma (HCC) model and CAM assay were used in this experiment. The effect of MK-AS on angiogenesis was evaluated by cell proliferation assay and hematoxylin-eosin (HE) staining.


RESULTS
MK-AS significantly inhibited human umbilical vein endothelial cells (HUVEC) and in situ human HCC growth. At the same time, MK-AS suppressed the angiogenesis both in human hepatocellular carcinoma cell line (HEPG2)-induced CAM and in situ human HCC tissues.


CONCLUSIONS
MK-AS is an effective antiangiogenesis agent in vivo.
Dai
Li-Cheng
LC
Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital, Huzhou 313000, Zhejiang Province, China. dlc@hzhospital.com
Wang
Xiang
X
Yao
Xing
X
Lu
Yong-Liang
YL
Ping
Jin-Liang
JL
He
Jian-Fang
JF
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000473:pathology; D002460:Cell Line; D002642:Chick Embryo; D016207:Cytokines / Q000235:genetics* / Q000627:therapeutic use; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy / Q000473:pathology; D000078792:Midkine; D009389:Neovascularization, Pathologic / Q000188:drug therapy*; D020319:Oligodeoxyribonucleotides, Antisense / Q000627:therapeutic use*; D023041:Xenograft Model Antitumor Assays
D016207:Cytokines; D020319:Oligodeoxyribonucleotides, Antisense; D000078792:Midkine