title: The taxonomy of Enterobacter sakazakii: proposal of a new genus Cronobacter gen. nov. and descriptions of Cronobacter sakazakii comb. nov. Cronobacter sakazakii subsp. sakazakii, comb. nov., Cronobacter sakazakii subsp. malonaticus subsp. nov., Cronobacter turicensis sp. nov., Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov. and Cronobacter genomospecies 1.
abstract: BACKGROUND
  Enterobacter sakazakii is an opportunistic pathogen that can cause infections such as necrotizing enterocolitis, bacteraemia, meningitis and brain abscess/lesions. When the species was defined in 1980, 15 biogroups were described and it was suggested that these could represent multiple species. In this study the taxonomic relationship of strains described as E. sakazakii was further investigated.
  
  
  RESULTS
  Strains identified as E. sakazakii were divided into separate groups on the basis of f-AFLP fingerprints, ribopatterns and full-length 16S rRNA gene sequences. DNA-DNA hybridizations revealed five genomospecies. The phenotypic profiles of the genomospecies were determined and biochemical markers identified.
  
  
  CONCLUSIONS
  This study clarifies the taxonomy of E. sakazakii and proposes a reclassification of these organisms.
authors: Quality and Safety Department, Nestlé Research Centre, Vers-Chez-les-Blanc, Lausanne, Switzerland. iversenc@fsafety.unizh.ch <iversenc@fsafety.unizh.ch>
authors: Carol
authors: C
authors: Iversen
authors: Angelika
authors: A
authors: Lehner
authors: Niall
authors: N
authors: Mullane
authors: Eva
authors: E
authors: Bidlas
authors: Ilse
authors: I
authors: Cleenwerck
authors: John
authors: J
authors: Marugg
authors: Séamus
authors: S
authors: Fanning
authors: Roger
authors: R
authors: Stephan
authors: Han
authors: H
authors: Joosten
mesh_terms: D001483:Base Sequence; D044083:Cronobacter sakazakii / Q000145:classification* / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004755:Enterobacteriaceae / Q000145:classification* / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010641:Phenotype; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D021521:Ribotyping; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S