Qualitative and quantitative analyses of nucleosides and nucleobases in Ganoderma spp. by HPLC-DAD-MS.
A high-performance liquid chromatography-diode array detector-mass spectrometry (HPLC-DAD-MS) analytical method was developed for detection of the nucleosides and nucleobases in two species of Lingzhi, the dried sporophore of Ganoderma lucidum and G. sinense. The method, combining advantages of both DAD and MS, was successfully used to qualitatively identify for six nucleosides namely, adenosine, cytidine, guanosine, inosine, thymidine, uridine and five nucleobases namely, adenine, guanine, hypoxanthine, thymine and uracil in Lingzhi samples. Quantitative analyses showed that uridine was the most abundant nucleoside in these Lingzhi samples and the contents of nine target analytes were found to be different in pileus and stipes of the fruiting bodies and among the different species of G. spp. The established method might apply as an alternative approach for the quality assessment of Lingzhi.
Gao
J L
JL
Institute of Chinese Medical Sciences, University of Macau, Taipa, Macau, China.
Leung
Kelvin S Y
KS
Wang
Y T
YT
Lai
C M
CM
Li
S P
SP
Hu
L F
LF
Lu
G H
GH
Jiang
Z H
ZH
Yu
Z L
ZL
D000225:Adenine / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D032902:Ganoderma / Q000737:chemistry*; D006147:Guanine / Q000032:analysis* / Q000737:chemistry; D019271:Hypoxanthine / Q000032:analysis* / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D009705:Nucleosides / Q000032:analysis* / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D013045:Species Specificity; D013678:Technology, Pharmaceutical / Q000379:methods; D013941:Thymine / Q000032:analysis* / Q000737:chemistry; D014498:Uracil / Q000032:analysis* / Q000737:chemistry
D009705:Nucleosides; D010936:Plant Extracts; D019271:Hypoxanthine; D014498:Uracil; D006147:Guanine; D000225:Adenine; D013941:Thymine