[6]-Gingerol prevents UVB-induced ROS production and COX-2 expression in vitro and in vivo.
[6]-Gingerol, a naturally occurring plant phenol, is one of the major components of fresh ginger (Zingiber officinale Roscoe, Zingiberaceae) and has diverse pharmacologic effects. Here, we describe its novel anti-oxidant, anti-apoptotic, and anti-inflammatory activities in vitro and in vivo. In vitro, pre-treatment with [6]-gingerol reduced UVB-induced intracellular reactive oxygen species levels, activation of caspase-3, -8, -9, and Fas expression. It also reduced UVB-induced expression and transactivation of COX-2. Translocation of NF-kappaB from cytosol to nucleus in HaCaT cells was inhibited by [6]-gingerol via suppression of IkappaBalpha phosphorylation (ser-32). Examination by EMSAs and immunohistochemistry showed that topical application of [6]-gingerol (30 microM) prior to UVB irradiation (5 kJ/m(2)) of hairless mice, also inhibited the induction of COX-2 mRNA and protein, as well as NF-kappaB translocation. These results suggest that [6]-gingerol could be an effective therapeutic agent providing protection against UVB-induced skin disorders.
Kim
Jin-Kyoung
JK
Department of Dermato-Immunology, College of Medicine, Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul, South Korea.
Kim
Younghwa
Y
Na
Kwang-Min
KM
Surh
Young-Joon
YJ
Kim
Tae-Yoon
TY
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002396:Catechols; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D018890:Chemoprevention; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D024202:Electrophoretic Mobility Shift Assay; D005233:Fatty Alcohols / Q000494:pharmacology*; D020939:Zingiber officinale / Q000737:chemistry; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects*; D008297:Male; D051379:Mice; D008812:Mice, Hairless; D009153:Mutagens / Q000494:pharmacology*; D016328:NF-kappa B / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects / Q000528:radiation effects; D021381:Protein Transport; D017382:Reactive Oxygen Species / Q000378:metabolism* / Q000528:radiation effects; D012867:Skin / Q000187:drug effects / Q000528:radiation effects; D012878:Skin Neoplasms / Q000517:prevention & control*; D014466:Ultraviolet Rays
D002396:Catechols; D005233:Fatty Alcohols; D009153:Mutagens; D016328:NF-kappa B; D017382:Reactive Oxygen Species; C007845:gingerol; D051546:Cyclooxygenase 2