title: Covalent grafting of fibronectin onto plasma-treated PTFE: influence of the conjugation strategy on fibronectin biological activity.
abstract: Surface coating of synthetic materials is often considered to improve biomedical devices biocompatibility. In this study, we covalently bound fibronectin (FN) onto ammonia plasma-treated PTFE via two crosslinkers, namely glutaric anhydride (GA) and sulfosuccinimidyl-4-(p-maleimidophenyl)butyrate (sulfo-SMPB). With respect to clean PTFE, cell adhesion increased markedly on both FN grafted surfaces, although it was twice higher on PTFE-GA-FN than on PTFE-SMPB-FN. ELISA experiments performed with a polyclonal antibody revealed that the amount of FN is identical on both surfaces while monoclonal antibody specific to the RGD binding site clearly demonstrated a greater availability when FN is surface grafted through GA. These results provide evidence of a variation in protein conformation correlated with the surface conjugation strategy.
authors: Unité de Biotechnologie et de Bioingénierie, Centre de recherche du CHUQ, Hôpital Saint-François d'Assise, 10 rue de l'Espinay, Québec, Canada, G1L 3L5.
authors: Karine
authors: K
authors: Vallières
authors: Eric
authors: E
authors: Petitclerc
authors: Gaétan
authors: G
authors: Laroche
mesh_terms: D000641:Ammonia; D000818:Animals; D001011:Aorta / Q000166:cytology; D001672:Biocompatible Materials; D002417:Cattle; D002448:Cell Adhesion; D042783:Endothelial Cells / Q000166:cytology; D004797:Enzyme-Linked Immunosorbent Assay; D005353:Fibronectins / Q000737:chemistry*; D005977:Glutarates / Q000737:chemistry; D010949:Plasma; D011138:Polytetrafluoroethylene / Q000737:chemistry*; D011487:Protein Conformation; D013388:Succinimides / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D005353:Fibronectins; D005977:Glutarates; D013388:Succinimides; D000641:Ammonia; C042424:succinimidyl 4-(4-maleimidophenyl)butyrate; D011138:Polytetrafluoroethylene; C035736:glutaric acid