title: Endocannabinoid signaling regulates spontaneous transmitter release from embryonic retinal amacrine cells.
abstract: GABAergic amacrine cells, cultured from embryonic chick retina, display spontaneous mini frequencies ranging from 0-4.6 Hz as a result of the release of quanta of transmitter from both synapses and autapses. We show here that at least part of this variation originates from differences in the degree to which endocannabinoids, endogenously generated within the culture, are present at terminals presynaptic to individual cells. Though all cells examined scored positive for cannabinoid receptor type I (CB1R), only those showing a low initial rate of spontaneous minis responded to CB1R agonists with an increase in mini frequency, caused by a Gi/o-mediated reduction in [cAMP]. Cells displaying a high initial rate of spontaneous minis, on the other hand, were unaffected by CB1R agonists, but they did show a rate decrease with CB1R antagonists. Such a regulation of spontaneous transmitter release by endocannabinoids might be important in network maintenance in amacrine cells and other inhibitory interneurons.
authors: Section of Neurobiology, Physiology and Behavior, Division of Biological Sciences, Davis, California 95616, USA.
authors: Ajithkumar
authors: A
authors: Warrier
authors: Martin
authors: M
authors: Wilson
mesh_terms: D025042:Amacrine Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D002118:Calcium / Q000502:physiology; D063385:Cannabinoid Receptor Modulators / Q000378:metabolism / Q000502:physiology*; D002478:Cells, Cultured; D002642:Chick Embryo; D000242:Cyclic AMP / Q000378:metabolism; D004594:Electrophysiology / Q000379:methods; D063388:Endocannabinoids; D007150:Immunohistochemistry; D018408:Patch-Clamp Techniques; D043884:Receptor, Cannabinoid, CB1 / Q000502:physiology; D012160:Retina / Q000166:cytology / Q000196:embryology*; D015398:Signal Transduction
chemical_list: D063385:Cannabinoid Receptor Modulators; D063388:Endocannabinoids; D043884:Receptor, Cannabinoid, CB1; D000242:Cyclic AMP; D002118:Calcium