title: A class I KNOX gene from the palm species Elaeis guineensis (Arecaceae) is associated with meristem function and a distinct mode of leaf dissection.
abstract: Class I Knotted-like homeobox (KNOX) transcription factors are important regulators of shoot apical meristem function and leaf morphology by their contribution to dissected leaf development. Palms are of particular interest as they produce dissected leaves generated by a distinct mechanism compared with eudicots. The question addressed here was whether class I KNOX genes might be involved in meristem function and leaf dissection in palms. Here, we characterized the EgKNOX1 gene from oil palm (Elaeis guineensis, Arecaceae) and compared it with available sequences from other plant species using phylogenetic analysis. Gene expression pattern was investigated using reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization. Functional analysis was carried out by ectopic expression in Arabidopsis and rice. EgKNOX1 was orthologous to STM from Arabidopsis and to OSH1 from rice. It was expressed in the central zone of both vegetative and reproductive meristems. During leaf development, its expression was associated with plications from which the leaflets originate. Different modes of leaf dissection are seen to involve a similar class of genes to control meristematic activities, which govern the production of dissected morphologies.
authors: IRD/CIRAD Palm Group, UMR 1098, Centre IRD Montpellier, 911 avenue Agropolis, 34394 Montpellier cedex 5, France.
authors: Stefan
authors: S
authors: Jouannic
authors: IRD/CIRAD Palm Group, UMR 1098, Centre IRD Montpellier, 911 avenue Agropolis, 34394 Montpellier cedex 5, France.
authors: Myriam
authors: M
authors: Collin
authors: IRD/CIRAD Palm Group, UMR 1098, Centre IRD Montpellier, 911 avenue Agropolis, 34394 Montpellier cedex 5, France.
authors: Benjamin
authors: B
authors: Vidal
authors: Plateau d'Histocytologie et d'Imagerie Cellulaire Végétale, IFR 127, TA/40/02, CIRAD, Avenue Agropolis, F-34398 Montpellier cedex 5, France.
authors: Jean-Luc
authors: JL
authors: Verdeil
authors: IRD/CIRAD Palm Group, UMR 1098, Centre IRD Montpellier, 911 avenue Agropolis, 34394 Montpellier cedex 5, France.
authors: James W
authors: JW
authors: Tregear
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D028023:Arecaceae / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D005801:Genes, Homeobox; D018398:Homeodomain Proteins / Q000235:genetics*; D018519:Meristem / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*
chemical_list: D018398:Homeodomain Proteins; D010940:Plant Proteins