Prevalence and distribution of HPV genotypes among HIV-infected women in Zambia.
We screened 145 HIV-infected non-pregnant women at a tertiary care centre in Lusaka, Zambia. Liquid-based cytology and human papillomavirus (HPV) genotyping with PGMY09/11 biotinylated primers (Roche Linear Array HPV genotyping test) maximised sensitivity of cytology and HPV assessments. Among high-risk (HR) types, HPV 52 (37.2%), 58 (24.1%) and 53 (20.7%) were more common overall than HPV 16 (17.2%) and 18 (13.1%) in women with high-grade squamous intraepithelial lesions or squamous cell carcinoma (SCC) on cytology. High-risk HPV types were more likely to be present in women with CD4+ cell counts <200 microl(-1) (odds ratios (OR): 4.9, 95% confidence intervals (CI): 1.4-16.7, P=0.01) and in women with high-grade or severe cervical cytological abnormalities (OR: 8.0, 95% CI: 1.7-37.4, P=0.008). Human papillomavirus diversity in high-grade lesions and SCC on cytology suggests that HPV 16- and 18-based vaccines may not be adequately polyvalent to induce protective immunity in this population.
Sahasrabuddhe
V V
VV
Vanderbilt University, 2215 Garland Avenue, 319 Light Hall, Nashville, TN 37232, USA. vikrant.sahasrabuddhe@vanderbilt.edu
Mwanahamuntu
M H
MH
Vermund
S H
SH
Huh
W K
WK
Lyon
M D
MD
Stringer
J S A
JS
Parham
G P
GP
D018791:CD4 Lymphocyte Count; D016001:Confidence Intervals; D005260:Female; D015658:HIV Infections / Q000453:epidemiology* / Q000276:immunology; D006801:Humans; D016017:Odds Ratio; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000453:epidemiology*; D015024:Zambia / Q000453:epidemiology