Role of hydrogen peroxide during the interaction between the hemibiotrophic fungal pathogen Septoria tritici and wheat.
Hydrogen peroxide (H(2)O(2)) is reported to inhibit biotrophic but benefit necrotrophic pathogens. Infection by necrotrophs can result in a massive accumulation of H(2)O(2) in hosts. Little is known of how pathogens with both growth types are affected (hemibiotrophs). The hemibiotroph, Septoria tritici, infecting wheat (Triticum aestivum) is inhibited by H(2)O(2) during the biotrophic phase, but a large H(2)O(2) accumulation occurs in the host during reproduction. Here, we infiltrated catalase, H(2)O(2) or water into wheat during the biotrophic or the necrotrophic phase of S. tritici and studied the effect of infection on host physiology to get an understanding of the survival strategy of the pathogen. H(2)O(2) removal by catalase at both early and late stages made plants more susceptible, whereas H(2)O(2) made them more resistant. H(2)O(2) is harmful to S. tritici throughout its life cycle, but it can be tolerated. The late accumulation of H(2)O(2) is unlikely to result from down-regulation of photosynthesis, but probably originates from damage to the peroxisomes during the general tissue collapse, which is accompanied by release of soluble sugars in a susceptible cultivar.
Shetty
Nandini P
NP
University of Copenhagen, Faculty of Life Sciences, Department of Plant Biology, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark.
Mehrabi
Rahim
R
Plant Research International, Wageningen University and Research Centre, PO Box 16, 6700 AA Wageningen, the Netherlands.
Lütken
Henrik
H
University of Copenhagen, Faculty of Life Sciences, Department of Plant Biology, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark.
Haldrup
Anna
A
University of Copenhagen, Faculty of Life Sciences, Department of Plant Biology, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark.
Kema
Gert H J
GHJ
Plant Research International, Wageningen University and Research Centre, PO Box 16, 6700 AA Wageningen, the Netherlands.
Collinge
David B
DB
University of Copenhagen, Faculty of Life Sciences, Department of Plant Biology, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark.
Jørgensen
Hans Jørgen Lyngs
HJL
University of Copenhagen, Faculty of Life Sciences, Department of Plant Biology, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark.
D001203:Ascomycota / Q000235:genetics / Q000378:metabolism*; D002241:Carbohydrates / Q000032:analysis; D002374:Catalase / Q000378:metabolism; D002734:Chlorophyll / Q000032:analysis; D004271:DNA, Fungal; D018506:Gene Expression Regulation, Plant; D006861:Hydrogen Peroxide / Q000378:metabolism*; D010788:Photosynthesis; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D014908:Triticum / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology*
D002241:Carbohydrates; D004271:DNA, Fungal; D002734:Chlorophyll; D006861:Hydrogen Peroxide; D002374:Catalase