Shared MHC class II-dependent melanoma ribosomal protein L8 identified by phage display.
Antigens recognized by T helper (Th) cells in the context of MHC class II molecules have vaccine potential against cancer and infectious agents. We have described previously a melanoma patient's HLA-DR7-restricted Th cell clone recognizing an antigen, which is shared among melanoma and glioma cells derived from various patients. Here, this antigen was cloned using a novel antigen phage display approach. The antigen was identified as the ribosomal protein L8 (RPL8). A peptide of RPL8 significantly stimulated proliferation and/or cytokine expression of the Th cell clone and lymphocytes in four of nine HLA-DR7(+) melanoma patients but not in healthy volunteers. The RPL8 antigen may represent a relevant vaccine target for patients with melanoma, glioma, and breast carcinoma whose tumors express this protein.
Swoboda
Rolf K
RK
Immunology Program, The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA.
Somasundaram
Rajasekharan
R
Caputo
Laura
L
Ochoa
Elizabeth M
EM
Gimotty
Phyllis A
PA
Marincola
Francesco M
FM
Van Belle
Patricia
P
Barth
Stephen
S
Elder
David
D
Guerry
DuPont
D
Czerniecki
Brian
B
Schuchter
Lynn
L
Vonderheide
Robert H
RH
Herlyn
Dorothee
D
D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology*; D019556:COS Cells; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D015809:HLA-DR7 Antigen / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation; D008545:Melanoma / Q000235:genetics / Q000276:immunology*; D019151:Peptide Library; D012269:Ribosomal Proteins / Q000235:genetics / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
D000951:Antigens, Neoplasm; D000939:Epitopes; D015809:HLA-DR7 Antigen; D019151:Peptide Library; D012269:Ribosomal Proteins; C075184:ribosomal protein L8, rat