title: [Leptin correlates with distribution of fatty tissue and plasma levels of insulin, testosterone and tumor necrosis factor alpha in perimenopausal women with increased testosterone level and central location of body fat].
abstract: OBJECTIVE
  The evaluation of the influence of testosterone and fat tissue distribution on the serum leptin levels in perimenopausal women.
  
  
  METHODS
  93 perimenopausal women without HRT (age: 51.0 +/- 8.8 yrs, FSH: 68.0 +/- 49.4 IU/l, estradiol: 38.3 +/- 37.0 ng/1) were divided into group A - 63 women with serum testosterone level < 0.6 ng/ml and group B--30 women with serum testosterone > or = 0.6 ng/ml. Each group was later divided according to WHR into subgroup I (AI and BI) (WHR < 0.8) and II (AII and BII) (WHR > or = 0.8).
  
  
  METHODS
  Basic fasting serum levels of LH, FSH, PRL, estradiol, insulin, hGH, IGF-I, IGFBP-1, IGFBP-3, leptin, testosterone, DHEAS TNF-alpha and SHBG were measured by RIA kits. Total cholesterol, HDL and LDL cholesterol, as well as triglycerides plasma levels were measured. Statistical evaluation was carried out by ANOVA and linear regression.
  
  
  RESULTS
  BMI, WHR and plasma DHEAS level were higher in group B vs. group A. The lowest hGH, HDL-cholesterol and the highest TNF-alpha levels were found in group BII. The relations leptin/BMI and leptin/body mass were found in each group. The inverse relation between leptin and IGFBP-1 was found in groups A and B. In group A the inverse relations leptin/HDL-cholesterol and leptin/ DHEAS were observed. In group B the direct leptin/testosterone and inverse leptin/IGF-I relations were found. In group AI the inverse leptin/DHEAS relation remained, while in group AII inverse leptin/HDL-cholesterol relation remained and reverse leptin/IGFBP-1 relation was significant. The direct leptin/testosterone, leptin/WHR and inverse leptin/TNF-alpha links were observed in group BII.
  
  
  CONCLUSIONS
  The serum leptin level was linked to WHR, serum testosterone, insulin, TNF-alpha levels only in groups of perimenopausal women with such cardiovascular risk factors as high WHR, overweight, high serum TNF-alpha and testosterone levels.
authors: Klinika Endokrynologii Ginekologicznej, Collegium Medicum, Uniwersytetu Jagiellońskiego w Krakowie. momilewi@cyf-kr.edu.pl
authors: Tomasz
authors: T
authors: Milewicz
authors: Józef
authors: J
authors: Krzysiek
authors: Agnieszka
authors: A
authors: Janczak-Saif
authors: Krystyna
authors: K
authors: Sztefko
mesh_terms: D050153:Abdominal Fat / Q000378:metabolism*; D015992:Body Mass Index; D004967:Estrogens / Q000097:blood; D005260:Female; D006801:Humans; D007328:Insulin / Q000097:blood*; D020738:Leptin / Q000097:blood*; D008875:Middle Aged; D009765:Obesity / Q000378:metabolism; D047648:Perimenopause / Q000378:metabolism*; D012044:Regression Analysis; D018709:Statistics, Nonparametric; D013739:Testosterone / Q000097:blood*; D014018:Tissue Distribution; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*
chemical_list: D004967:Estrogens; D007328:Insulin; D020738:Leptin; D014409:Tumor Necrosis Factor-alpha; D013739:Testosterone