An ERF transcription factor in Medicago truncatula that is essential for Nod factor signal transduction.
Rhizobial bacteria activate the formation of nodules on the appropriate host legume plant, and this requires the bacterial signaling molecule Nod factor. Perception of Nod factor in the plant leads to the activation of a number of rhizobial-induced genes. Putative transcriptional regulators in the GRAS family are known to function in Nod factor signaling, but these proteins have not been shown to be capable of direct DNA binding. Here, we identify an ERF transcription factor, ERF Required for Nodulation (ERN), which contains a highly conserved AP2 DNA binding domain, that is necessary for nodulation. Mutations in this gene block the initiation and development of rhizobial invasion structures, termed infection threads, and thus block nodule invasion by the bacteria. We show that ERN is necessary for Nod factor-induced gene expression and for spontaneous nodulation activated by the calcium- and calmodulin-dependent protein kinase, DMI3, which is a component of the Nod factor signaling pathway. We propose that ERN is a component of the Nod factor signal transduction pathway and functions downstream of DMI3 to activate nodulation gene expression.
Middleton
Patrick H
PH
Department of Disease and Stress Biology, John Ines Centre, Norwich NR4 7UH, UK.
Jakab
Júlia
J
Penmetsa
R Varma
RV
Starker
Colby G
CG
Doll
Jake
J
Kaló
Péter
P
Prabhu
Radhika
R
Marsh
John F
JF
Mitra
Raka M
RM
Kereszt
Attila
A
Dudas
Brigitta
B
VandenBosch
Kathryn
K
Long
Sharon R
SR
Cook
Doug R
DR
Kiss
Gyorgy B
GB
Oldroyd
Giles E D
GE
D000595:Amino Acid Sequence; D018506:Gene Expression Regulation, Plant; D008070:Lipopolysaccharides / Q000378:metabolism*; D029902:Medicago / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
D008070:Lipopolysaccharides; D010940:Plant Proteins; D014157:Transcription Factors