Monod kinetics for aerobic biodegradation of petroleum hydrocarbons in unsaturated soil microcosms.
We use Monod kinetics to calibrate previously published data that documentthe aerobic biodegradation of hydrocarbon vapors in soil microcosms from a weathered petroleum spill site. Monod kinetics offer insight into biodegradation mechanics because they address biomass growth as well as substrate depletion. A blend of five aromatics and five alkanes dose the microcosm sets at four strengths, and a finite difference model describes the response superimposed across the constituent substrates. An observed initial biomass X0 of 125 g biomass/m(3) soil moisture and an endogendous decay rate b of 0.102 day(-1) calibrate all four dosages and agree with heterotrophic plate counts. Common maximum specific growth rates microMJ and half saturation constants KSJ calibrate each constituent across the four dosages. The biodegradable alkanes exhibit microMJ values ranging from 0.0190 to 0.0996 day(-1), while the aromatic rates vary from 0.0946 to 0.322 day(-1). One of the alkanes (2,2,4-trimethylpentane) is recalcitrant. The half saturation constants range from 0.000083 to 0.000355 g substrate/m(3) soil moisture for the biodegradable alkanes, which imply zero-order kinetics. The aromatic KSJ values vary from 5.02 to 14.3 g substrate/m(3) soil moisture, and suggest first-order kinetics. The yield YJ increases with dosage concentration for all the biodegradable constituents, varying from 0.0533 to 1.58 g biomass/g substrate.
Ostendorf
David W
DW
Civil and Environmental Engineering Department, University of Massachusetts, Amherst, Massachusetts 01003, USA. ostendorf@ecs.umass.edu
Schoenberg
Theodore H
TH
Hinlein
Erich S
ES
Long
Sharon C
SC
D001420:Bacteria, Aerobic / Q000254:growth & development / Q000378:metabolism*; D001673:Biodegradation, Environmental; D018533:Biomass; D007700:Kinetics; D008956:Models, Chemical; D010578:Petroleum / Q000378:metabolism*; D012987:Soil / Q000032:analysis*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
D010578:Petroleum; D012987:Soil; D012989:Soil Pollutants