WC1+ gammadelta T cell memory population is induced by killed bacterial vaccine.
Limited studies have addressed the ability of gammadelta T cells to become memory populations. We previously demonstrated that WC1.1(+) gammadelta T cells from ruminants vaccinated with killed Leptospira borgpetersenii proliferate and produce IFN-gamma in recall responses. Here we show that this response is dependent upon antigen-responsive CD4 T cells, at least across transwell membranes; this requirement cannot be replaced by IL-2. The response was also dependent upon in vivo priming, since gammadelta T cells from leptospira vaccine-naive animals did not respond to antigen even when co-cultured across membranes from antigen-responsive PBMC. Gammadelta T cells were the major antigen-responding T cell population for the first 4 wks following vaccination and replicated more rapidly than CD4 T cells. Primed WC1(+) gammadelta T cells circulated as CD62L(hi)/CD45RO(int)/CD44(lo), characteristics of T(CM) cells. When stimulated with antigen, they decreased CD62L, increased CD44 and CD25, and had no change in CD45RO expression. These changes paralleled those of the leptospira antigen-responsive CD4 T cells but differed from those of gammadelta T cells proliferating to mitogen stimulation. This system for in vivo gammadelta T cell priming is unique, since it relies on a killed antigen to induce memory and may be pertinent to designing vaccines that require type 1 pro-inflammatory cytokines.
Blumerman
Seth L
SL
University of Massachusetts, Amherst, MA 01003, USA.
Herzig
Carolyn T A
CT
Baldwin
Cynthia L
CL
D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000382:microbiology; D002417:Cattle; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D007156:Immunologic Memory; D007371:Interferon-gamma / Q000276:immunology; D016953:Leptospiraceae / Q000276:immunology; D007922:Leptospirosis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000382:microbiology; D015164:Vaccines, Inactivated
D001428:Bacterial Vaccines; D016692:Receptors, Antigen, T-Cell, gamma-delta; D015164:Vaccines, Inactivated; D007371:Interferon-gamma