Saturated and monounsaturated fatty acids increase interleukin-10 production in rat hepatocytes.
Interleukin-10 (IL-10) is a potent anti-inflammatory cytokine, but it still remains unknown whether saturated or unsaturated fatty acid affects IL-10 production in hepatocytes that contribute to lipid metabolism. Primary rat hepatocyte cultures were treated with different fatty acids (18:0 stearic acid, 18:1 oleic acid; 18:2 linoleic acid, 18:3 linolenic acid) at 300 microM for 24 hours. The concentrations of IL-2, IL-10, GM-CSF and IFN-gamma in the medium were detected by multiplex cytokine array. IL-10 was significantly increased with treatment of stearic acid and oleic acid. Production of IL-10 by saturated and monounsaturated fatty acids in hepatocytes may be one of the reasons why the lard oil had less inflammation in the hepatic steatosis animal models.
Nishitani
Yoko
Y
Department of Legal Medicine, Sapporo Medical University School of Medicine, South 1 West 17, Sapporo 060-8556, Japan.
Okazaki
Shunichiro
S
Imabayashi
Kiyomi
K
Katada
Ryuichi
R
Umetani
Kojiro
K
Yajima
Hidetaka
H
Matsumoto
Hiroshi
H
D000818:Animals; D002478:Cells, Cultured; D005227:Fatty Acids / Q000494:pharmacology*; D005229:Fatty Acids, Monounsaturated / Q000494:pharmacology*; D022781:Hepatocytes / Q000378:metabolism*; D016753:Interleukin-10 / Q000096:biosynthesis*; D050356:Lipid Metabolism / Q000187:drug effects; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D016753:Interleukin-10