Effect of HbS in the determination of HbA2 with the Biorad Variant II analyzer.
OBJECTIVE
The effect of the presence of HbS in the determination of HbA2 using the Biorad Variant II analyzer.


METHODS
The effect of HbS presence in the samples was quantified using the HELENA SAS-MX alkaline gel electrophoresis kit as the reference method.


RESULTS
The %HbA2 values from the Variant II analyzer and the HELENA SAS-MX alkaline gel electrophoresis kit show a good linear correlation in the absence of HbS. A strong positive bias in the %HbA2 values from the Variant II is apparent in the presence of HbS in the samples, when compared to the alkaline electrophoresis gel.


CONCLUSIONS
The Variant II analyzer gives reliable results for %HbA2 determination when no HbS is detectable in the samples. When HbS is present, the gel electrophoresis method gives more accurate results.
Kalleas
Christoforos
C
Hematology Laboratory, General Hospital of Poligiros, Poligiros, Greece.
Tentes
Ioannis
I
Margaritis
Dimitrios
D
Anagnostopoulos
Kostas
K
Toli
Athina
A
Pendilas
Dimitrios
D
Bourikas
Georgios
G
Tsatalas
Constantinos
C
Kortsaris
Alexandros H
AH
D001322:Autoanalysis; D002847:Chromatography, Agarose; D002851:Chromatography, High Pressure Liquid; D005189:False Positive Reactions; D006443:Hemoglobin A2 / Q000032:analysis* / Q000302:isolation & purification; D006451:Hemoglobin, Sickle / Q000494:pharmacology*; D006801:Humans
D006451:Hemoglobin, Sickle; D006443:Hemoglobin A2