Covalent grafting of fibronectin onto plasma-treated PTFE: influence of the conjugation strategy on fibronectin biological activity.
Surface coating of synthetic materials is often considered to improve biomedical devices biocompatibility. In this study, we covalently bound fibronectin (FN) onto ammonia plasma-treated PTFE via two crosslinkers, namely glutaric anhydride (GA) and sulfosuccinimidyl-4-(p-maleimidophenyl)butyrate (sulfo-SMPB). With respect to clean PTFE, cell adhesion increased markedly on both FN grafted surfaces, although it was twice higher on PTFE-GA-FN than on PTFE-SMPB-FN. ELISA experiments performed with a polyclonal antibody revealed that the amount of FN is identical on both surfaces while monoclonal antibody specific to the RGD binding site clearly demonstrated a greater availability when FN is surface grafted through GA. These results provide evidence of a variation in protein conformation correlated with the surface conjugation strategy.
Vallières
Karine
K
Unité de Biotechnologie et de Bioingénierie, Centre de recherche du CHUQ, Hôpital Saint-François d'Assise, 10 rue de l'Espinay, Québec, Canada, G1L 3L5.
Petitclerc
Eric
E
Laroche
Gaétan
G
D000641:Ammonia; D000818:Animals; D001011:Aorta / Q000166:cytology; D001672:Biocompatible Materials; D002417:Cattle; D002448:Cell Adhesion; D042783:Endothelial Cells / Q000166:cytology; D004797:Enzyme-Linked Immunosorbent Assay; D005353:Fibronectins / Q000737:chemistry*; D005977:Glutarates / Q000737:chemistry; D010949:Plasma; D011138:Polytetrafluoroethylene / Q000737:chemistry*; D011487:Protein Conformation; D013388:Succinimides / Q000737:chemistry
D001672:Biocompatible Materials; D005353:Fibronectins; D005977:Glutarates; D013388:Succinimides; D000641:Ammonia; C042424:succinimidyl 4-(4-maleimidophenyl)butyrate; D011138:Polytetrafluoroethylene; C035736:glutaric acid