Involvement of p38 mitogen-activated protein kinase in E. coli-induced U937 apoptosis.
OBJECTIVE
To investigate whether the effect of E. coli on U937 cell lines apoptosis is mediated via p38 mitogen-activated protein kinase (MAPK) activation.


METHODS
The U937 cell lines were treated with E. coli at different time or together with SB203580, an inhibitor for p38. Cell apoptosis was analyzed by flow cytometry. p38 activities were detected by Western blotting.


RESULTS
E. coli induced apoptosis in cultured U937 cell lines in a time-dependent manner. The phosphorylation of p38 was induced after 10 minutes infection, reached the peak after 20 minutes, and started to decline after 30 minutes. In contrast, the level of total p38 protein was not changed in whole experimental period. Inhibition of p38 with SB203580 significantly inhibited E. coli induced apoptosis in U937 cells.


CONCLUSIONS
The activation of the p38 MAPK in U937 cell lines by E. coli is a major pathway to mediate the apoptosis.
Wang
Jia-He
JH
Department of Geriatrics, Second Affiliated Hospital, China Medical University, Shenyang 110004.
Zhou
Yi-Jun
YJ
He
Ping
P
Chen
Bai-Yi
BY
D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004926:Escherichia coli; D005434:Flow Cytometry; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D007700:Kinetics; D011725:Pyridines / Q000494:pharmacology; D020298:U937 Cells / Q000382:microbiology / Q000473:pathology; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
D004791:Enzyme Inhibitors; D007093:Imidazoles; D011725:Pyridines; D048051:p38 Mitogen-Activated Protein Kinases; C093642:SB 203580