Telomere length status of somatic cell sheep clones and their offspring.
This study was carried out to determine the telomere length status of sheep clones and their offspring, and to examine telomere dynamics and chromosomal abnormalities in culture propagated donor cells. Skin samples were collected from somatic cell nuclear transfer-derived sheep clones, and three of their progeny generated by natural mating. Samples were collected from control animals (n = 35), spanning in age from 1 month to 36 months of age. Genomic DNA was extracted from cell/tissue samples and their telomere lengths were assessed by terminal restriction fragment (TRF) analysis. Results revealed: that (a) sheep clones derived from cultured somatic cells have shortened telomere lengths compared to age-matched controls; (b) the offspring derived from natural mating between clones had normal telomere lengths compared to their age-matched counterparts; and donor cell cultures beyond 20 population doublings had significantly (P < 0.05) shortened telomeres and exhibited a higher numerical and structural chromosomal abnormalities.
Alexander
Basil
B
Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
Coppola
Gianfranco
G
Perrault
Steven D
SD
Peura
Teija T
TT
Betts
Dean H
DH
King
W Allan
WA
D000367:Age Factors; D000818:Animals; D002478:Cells, Cultured; D002875:Chromosomes / Q000648:ultrastructure*; D002999:Clone Cells / Q000378:metabolism; D004247:DNA / Q000032:analysis / Q000648:ultrastructure; D005347:Fibroblasts / Q000378:metabolism / Q000648:ultrastructure; D016678:Genome / Q000235:genetics; D009693:Nucleic Acid Hybridization; D034561:Sheep, Domestic / Q000235:genetics*; D012867:Skin / Q000166:cytology; D016615:Telomere / Q000648:ultrastructure*
D004247:DNA