High-pressure cryocooling for capillary sample cryoprotection and diffraction phasing at long wavelengths.
Crystal cryocooling is usually employed to reduce radiation damage during X-ray crystallography. Recently, a high-pressure cryocooling method has been developed which results in excellent diffraction-quality crystals without the use of penetrative cryoprotectants. Three new developments of the method are presented here: (i) Xe-He high-pressure cryocooling for Xe SAD phasing, (ii) native sulfur SAD phasing and (iii) successful cryopreservation of crystals in thick-walled capillaries without additional cryoprotectants other than the native mother liquor. These developments may be useful for structural solution of proteins without the need for selenomethionine incorporation and for high-throughput protein crystallography.
Kim
Chae Un
CU
Field of Biophysics, Cornell University, Ithaca, NY 14853, USA.
Hao
Quan
Q
Gruner
Sol M
SM
D000818:Animals; D003080:Cold Temperature; D015925:Cryopreservation; D003460:Crystallization; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D010179:Pancreas / Q000201:enzymology; D010196:Pancreatic Elastase / Q000737:chemistry*; D011312:Pressure; D013552:Swine
D010196:Pancreatic Elastase