title: Excretory-secretory products from Fasciola hepatica induce eosinophil apoptosis by a caspase-dependent mechanism.
abstract: Eosinophils (Eo) are known to be important effector cells in the host defense against helminth parasites. Excretory-secretory products (ESP) released by helminths have shown wide immunomodulatory properties, such as the induction of cellular apoptosis. We investigated the ability of ESP from Fasciola hepatica to induce Eo apoptosis. In this work, we observed that ESP induced an early apoptosis of rat peritoneal eosinophils and that this phenomenon was time- and concentration-dependent. Furthermore, we demonstrated that activation of protein tyrosine kinases (TyrK) and caspases were necessary to mediate the Eo apoptosis induced by the ESP, and that carbohydrate components present in these antigens were involved in this effect. We have described for the first time the ability of ESP from F. hepatica to modify the viability of Eo by apoptosis induction. Besides that, we have observed Eo apoptosis in the liver of rats 21 days after F. hepatica infection. The diminution in Eo survival in early infection could be a parasite strategy in order to prevent a host immune response.
authors: Parasitología, Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, CIBICI-CONICET, Medina Allende y Haya de la Torre, Ciudad Universitaria, 5000 Cordoba, Argentina.
authors: Marianela C
authors: MC
authors: Serradell
authors: Lorena
authors: L
authors: Guasconi
authors: Laura
authors: L
authors: Cervi
authors: Laura S
authors: LS
authors: Chiapello
authors: Diana T
authors: DT
authors: Masih
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000502:physiology*; D017209:Apoptosis; D020169:Caspases / Q000378:metabolism*; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D004804:Eosinophils / Q000276:immunology* / Q000469:parasitology / Q000502:physiology*; D005210:Fasciola hepatica / Q000276:immunology / Q000472:pathogenicity*; D005211:Fascioliasis / Q000276:immunology* / Q000469:parasitology / Q000503:physiopathology; D046529:Microscopy, Electron, Transmission; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D051381:Rats
chemical_list: D000947:Antigens, Helminth; D011505:Protein-Tyrosine Kinases; D020169:Caspases