[HLA class I antibody screening and typing in the sera of dialyzed patients with CDC and ELISA techniques: association with graft survival].
OBJECTIVE
As generally accepted, HLA antibody screening and typing in dialyzed kidney patients are important for the successful outcome of transplantation.


METHODS
Authors investigated the serum samples of 133 patients from the north-eastern region of Hungary for HLA class I antibodies with complement dependent cytotoxicity(CDC) and solid phase enzyme linked immunosorbent (ELISA) techniques (Biotest Abscreen and Abident class I), respectively.


RESULTS
The comparison of the results revealed a strong correlation (c2=7,23, p < 0,01) for antibody screening. However, the two methods detect two slightly different antibody populations. The deviation is mainly to be attributed to the higher sensitivity of ELISA, which resulted in antibody positivity of some CDC negative sera (25/106-23,6%), despite the fact that ELISA detects IgG alone. The authors investigated 56 patients, who had already undergone transplantation but got back on the waiting list later and found that average graft survival was shorter in the group of patients with antibody, regardless to the method applied. However, the difference in graft survival proved to be more significant if the patients were assorted to groups by ELISA rather than CDC positivity (ELISA: 58,4 vs 111,2 months - p < 0,001, CDC:50,5 vs 75,6 months - p > 0,05, n.s.).


CONCLUSIONS
This observation suggests that the clinical relevance of antibodies detected by ELISA alone -- due to its higher sensitivity -- are not to be ignored. In this context authors emphasize the HLA class I antibody screening and typing in the post-transplantation period.
Kozma
Laszlo
L
Országos Vérellátó Szolgálat Debreceni Regionális Központ, Debrecen, Hungary. kozma.laszlo@ovsz.hu
Bohaty
Ilona
I
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D003165:Complement System Proteins / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006085:Graft Survival; D015395:Histocompatibility Antigens Class I; D006801:Humans; D006814:Hungary; D007518:Isoantibodies; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis; D012703:Serotyping
D015395:Histocompatibility Antigens Class I; D007518:Isoantibodies; D003165:Complement System Proteins