Mutational analysis of the ribosome.
The ribosome is responsible for protein synthesis, the translation of the genetic code, in all living organisms. Ribosomes are composed of RNA (ribosomal RNA) and protein (ribosomal protein). Soluble protein factors bind to the ribosome and facilitate different phases of translation. Genetic approaches have proved useful for the identification and characterization of the structural and functional roles of specific nucleotides in ribosomal RNA and of specific amino acids in ribosomal proteins and in ribosomal factors. This chapter summarizes examples of mutations identified in ribosomal RNA, ribosomal proteins, and ribosomal factors.
Triman
Kathleen L
KL
Department of Biology, Franklin and Marshall College, Lancaster, PA 17604, USA.
D000818:Animals; D001483:Base Sequence; D004252:DNA Mutational Analysis; D006801:Humans; D009154:Mutation; D009690:Nucleic Acid Conformation; D010445:Peptide Elongation Factors / Q000235:genetics; D010448:Peptide Initiation Factors / Q000235:genetics; D010454:Peptide Termination Factors / Q000235:genetics; D021122:Protein Subunits / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000737:chemistry / Q000502:physiology; D012338:RNA, Ribosomal, 23S / Q000032:analysis / Q000737:chemistry / Q000502:physiology; D012269:Ribosomal Proteins / Q000235:genetics; D012270:Ribosomes / Q000235:genetics*; D017423:Sequence Analysis, RNA
D010445:Peptide Elongation Factors; D010448:Peptide Initiation Factors; D010454:Peptide Termination Factors; D021122:Protein Subunits; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D012269:Ribosomal Proteins; C087267:ribosome releasing factor