Development of a serological assay based on a synthetic peptide selected from the VP0 capsid protein for detection of human parechoviruses.
A serological enzyme-linked immunosorbent assay was developed using a synthetic peptide from the VP0 protein of human parechoviruses (HPeVs). Seroprevalence for HPeVs was 70% in children of < or = 5 years of age and 95% in adults. For children from whom serial sera were sampled, seropositivity increased from 22% to 88% between 2 and 24 months of age.
Abed
Yacine
Y
Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Québec and Laval University, Québec City, Québec, Canada.
Wolf
Dana
D
Dagan
Ron
R
Boivin
Guy
G
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000276:immunology; D036022:Capsid Proteins / Q000737:chemistry / Q000276:immunology*; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007223:Infant; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D030061:Parechovirus / Q000276:immunology*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology*; D010850:Picornaviridae Infections / Q000453:epidemiology* / Q000276:immunology / Q000821:virology; D016036:Seroepidemiologic Studies
D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D010455:Peptides