Discovery of a novel human picornavirus in a stool sample from a pediatric patient presenting with fever of unknown origin.
Fever of unknown origin (FUO) is a serious problem in the United States. An unidentified agent was cultured from the stool of an infant who presented with FUO. This virus showed growth in HFDK cells and suckling mice. Using DNase sequence-independent single-primer amplification, we identified several nucleotide sequences with a high homology to Theiler's murine encephalomyelitis virus. Nearly full-length viral genome sequencing and phylogenetic analysis demonstrate that this virus is a member of the Cardiovirus genus of the Picornaviridae family.
Jones
Morris S
MS
Clinical Investigation Facility, David Grant USAF Medical Center, 101 Bodin Circle, Travis AFB, CA 94535, USA. drmorrisj@yahoo.com
Lukashov
Vladimir V
VV
Ganac
Robert D
RD
Schnurr
David P
DP
D000595:Amino Acid Sequence; D005243:Feces / Q000821:virology*; D005260:Female; D005335:Fever of Unknown Origin / Q000150:complications* / Q000821:virology; D016679:Genome, Viral; D006801:Humans; D007223:Infant; D010802:Phylogeny; D010849:Picornaviridae / Q000145:classification* / Q000302:isolation & purification*; D010850:Picornaviridae Infections / Q000150:complications* / Q000821:virology*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D014764:Viral Proteins