title: A novel double-stable T-REx/gb cell line expressing glycoprotein B of herpes simplex virus 1.
abstract: We established and characterized a new stable T-REx/gB cell line expressing gB of HSV-1 under tetracycline (Tet) control. The expression of complete gB (120 K) in T-REx/gB cells was detected by Western blot analysis with anti-HSV-1/ANG/gB monoclonal antibody as early as 2 days after Tet induction. Inducibility and tightness of Tet-regulated gB expression in T-REx/gB cell line was shown to be preserved after long-term culture (2 months) and after numerous freezing/thawing cycles as well. In this study, we described the conditions required for the generation of the T-REx/gB cell line, which can be useful as a host for the isolation and propagation of HSV-1 recombinant viruses defective in gB gene.
authors: Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
authors: R
authors: R
authors: Tangelmayer
authors: V
authors: V
authors: Durmanová
authors: C W
authors: CW
authors: Knopf
authors: M
authors: M
authors: Kúdelová
authors: I
authors: I
authors: Rezuchová
mesh_terms: D015153:Blotting, Western; D002460:Cell Line / Q000821:virology*; D005786:Gene Expression Regulation; D018259:Herpesvirus 1, Human / Q000235:genetics; D006801:Humans; D008856:Microscopy, Fluorescence; D013752:Tetracycline / Q000494:pharmacology; D014759:Viral Envelope Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus; D013752:Tetracycline