[Effect of 2-phenoxyethanol on potency of Sabin inactivated poliomyelitis vaccine and its safety].
OBJECTIVE
To investigate the effect of 2-phenoxyethanol on potency of Sabin inactivated poliomyelitis vaccine (IPV).


METHODS
Sabin IPV samples containing 5 mg or 7 mg 2-phenoxyethanol each dosage respectively were placed separately at 4 degrees C, 37 degrees C for 2 days and 7 days. D-antigen contents were tested with ELISA method. Then neutralizing antibodies in mice and guinea pigs were detected. The safety experiment was performed according to unusual toxicity test of China requirement for biological product.


RESULTS
After addition of 2-phenoxyethanol, the I, II, and III D-antigen contents of Sabin IPV did not change. The antibody levels in mice and guinea pigs were not different between experimental group and control group. Animals were safe during observation period.


CONCLUSIONS
2-Phenoxyethanol had no effect on potency and safety of Sabin IPV. It can be used as antiseptic for Sabin IPV.
Bian
Chuan-xiu
CX
Institute of Medical Biology, Chinese Academy of Medical Science, Kunming 650118, China.
Jiang
Shu-de
SD
Yang
Jian-yong
JY
Sun
Ming-bo
MB
Xie
Ming-xue
MX
Zhang
Xin-wen
XW
Liao
Guo-yang
GY
Li
Wei-dong
WD
D000818:Animals; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage / Q000494:pharmacology / Q000633:toxicity; D000956:Antigens, Viral / Q000032:analysis / Q000276:immunology; D001835:Body Weight / Q000187:drug effects; D002522:Chlorocebus aethiops; D004355:Drug Stability; D004797:Enzyme-Linked Immunosorbent Assay; D005026:Ethylene Glycols / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D006168:Guinea Pigs; D051379:Mice; D009500:Neutralization Tests; D011054:Poliovirus Vaccine, Inactivated / Q000008:administration & dosage / Q000276:immunology* / Q000633:toxicity; D014709:Vero Cells
D000891:Anti-Infective Agents, Local; D000956:Antigens, Viral; D005026:Ethylene Glycols; D011054:Poliovirus Vaccine, Inactivated; C005398:phenoxyethanol