New insights into the Enterococcus faecalis CroRS two-component system obtained using a differential-display random arbitrarily primed PCR approach.
Using a modified random arbitrarily primed PCR approach, the operon encoding the Enterococcus faecalis JH2-2 CroRS two-component regulatory system was shown to be repressed during stationary phase, and a CroRS-regulated operon (glnQHMP) was identified. Gel retardation assays showed that the CroR regulator binds specifically to the glnQHMP promoter.
Le Breton
Yoann
Y
Laboratoire Microbiologie de l'Environnement, EA 956, USC INRA 2017, IRBA, Université de Caen, France. ylebreton@umaryland.edu
Muller
Cécile
C
Auffray
Yanick
Y
Rincé
Alain
A
D001426:Bacterial Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics; D004269:DNA, Bacterial / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D013293:Enterococcus faecalis / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D009876:Operon / Q000235:genetics; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D015398:Signal Transduction / Q000235:genetics; D014157:Transcription Factors / Q000378:metabolism*
D001426:Bacterial Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D014157:Transcription Factors; C037853:glutamine transport proteins