title: Local and circulating microchimerism is associated with hypersensitivity pneumonitis.
abstract: BACKGROUND
  Hypersensitivity pneumonitis (HP) is a lymphocytic alveolitis provoked by exposure to a variety of antigens. However, the disease occurs in only a subset of exposed individuals, suggesting that additional factors may be involved. Microchimerism has been implicated in the pathogenesis of autoimmune diseases, especially in those showing increased incidence after childbearing age.
  
  
  OBJECTIVE
  To evaluate the presence of circulating and local microchimeric cells in female patients with HP.
  
  
  METHODS
  Male microchimerism was examined in 103 patients with HP, 30 with idiopathic pulmonary fibrosis (IPF), and 43 healthy women. All of them had given birth to at least one son, with no twin siblings, blood transfusions, or transplants. Microchimerism was examined by dot blot hybridization (peripheral blood), and by fluorescence in situ hybridization in bronchoalveolar lavage cells and lungs.
  
  
  RESULTS
  Blood microchimerism was found in 33% of the patients with HP in comparison with 10% in those with IPF (p = 0.019) and 16% in healthy women (p = 0.045). Patients with HP with microchimerism showed a significant reduction of diffusing capacity of carbon monoxide (Dl(CO); 53.5 +/- 11.9% vs. 65.2 +/- 19.7%; p = 0.02) compared with patients with HP without microchimerism. In bronchoalveolar lavage cells, microchimerism was detected in 9 of 14 patients with HP compared with 2 of 10 patients with IPF (p = 0.047). Cell sorting revealed that microchimeric cells were either macrophages or CD4+ or CD8+ T cells. Male microchimeric cells were also found in the five HP lungs examined by fluorescence in situ hybridization.
  
  
  CONCLUSIONS
  Our findings (1) demonstrate that patients with HP exhibit increased frequency of fetal microchimerism, (2) confirm the multilineage capacity of microchimeric cells, and (3) suggest that microchimeric cells may increase the severity of the disease.
authors: Instituto Nacional de Enfermedades Respiratorias, Mexico, DF, Mexico.
authors: Martha L
authors: ML
authors: Bustos
authors: Sara
authors: S
authors: Frías
authors: Sandra
authors: S
authors: Ramos
authors: Andrea
authors: A
authors: Estrada
authors: José Luis
authors: JL
authors: Arreola
authors: Felipe
authors: F
authors: Mendoza
authors: Miguel
authors: M
authors: Gaxiola
authors: Mauricio
authors: M
authors: Salcedo
authors: Annie
authors: A
authors: Pardo
authors: Moisés
authors: M
authors: Selman
mesh_terms: D000328:Adult; D000542:Alveolitis, Extrinsic Allergic / Q000235:genetics / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D016022:Case-Control Studies; D018797:Cell Cycle Proteins / Q000235:genetics*; D002678:Chimera / Q000276:immunology*; D046528:Chimerism; D005260:Female; D006801:Humans; D008168:Lung / Q000166:cytology; D008297:Male; D008875:Middle Aged; D009679:Nuclear Family; D011658:Pulmonary Fibrosis / Q000235:genetics
chemical_list: D018797:Cell Cycle Proteins; C497749:TSPY1 protein, human