title: The phosphatidylinositol 3-kinase signaling pathway exerts protective effects during sepsis by controlling C5a-mediated activation of innate immune functions.
abstract: The PI3K/Akt signaling pathway has been recently suggested to have controversial functions in models of acute and chronic inflammation. Our group and others have reported previously that the complement split product C5a alters neutrophil innate immunity and cell signaling during the onset of sepsis and is involved in PI3K activation. We report in this study that in vivo inhibition of the PI3K pathway resulted in increased mortality in septic mice accompanied by strongly elevated serum levels of TNF-alpha, IL-6, MCP-1, and IL-10 during sepsis as well as decreased oxidative burst activity in blood phagocytes. PI3K inhibition in vitro resulted in significant increases in TLR-4-mediated generation of various proinflammatory cytokines in neutrophils, whereas the opposite effect was observed in PBMC. Oxidative burst and phagocytosis activity was significantly attenuated in both neutrophils and monocytes when PI3K activation was blocked. In addition, PI3K inhibition resulted in strongly elevated TLR-4-mediated generation of IL-1beta and IL-8 in neutrophils when these cells were co-stimulated with C5a. C5a-induced priming effects on neutrophil and monocyte oxidative burst activity as well as C5a-induced phagocytosis in neutrophils were strongly reduced when PI3K activation was blocked. Our data suggest that the PI3K/Akt signaling pathway controls various C5a-mediated effects on neutrophil and monocyte innate immunity and exerts an overall protective effect during experimental sepsis.
authors: Department of Trauma Surgery, Hannover Medical School, Carl Neuberg Strasse 1, 30625 Hannover, Germany.
authors: Christiane D
authors: CD
authors: Wrann
authors: Navid A
authors: NA
authors: Tabriz
authors: Tanja
authors: T
authors: Barkhausen
authors: Andreas
authors: A
authors: Klos
authors: Martijn
authors: M
authors: van Griensven
authors: Hans C
authors: HC
authors: Pape
authors: Daniel O
authors: DO
authors: Kendoff
authors: Renfeng
authors: R
authors: Guo
authors: Peter A
authors: PA
authors: Ward
authors: Christian
authors: C
authors: Krettek
authors: Niels C
authors: NC
authors: Riedemann
mesh_terms: D000818:Animals; D015936:Complement C5a / Q000494:pharmacology / Q000502:physiology*; D016207:Cytokines / Q000097:blood; D006801:Humans; D007113:Immunity, Innate; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology; D051379:Mice; D009000:Monocytes / Q000201:enzymology / Q000276:immunology; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology / Q000276:immunology; D010586:Phagocytes / Q000276:immunology; D010587:Phagocytosis; D019869:Phosphatidylinositol 3-Kinases / Q000502:physiology*; D000081082:Phosphoinositide-3 Kinase Inhibitors; D051057:Proto-Oncogene Proteins c-akt / Q000037:antagonists & inhibitors / Q000502:physiology*; D016897:Respiratory Burst; D018805:Sepsis / Q000276:immunology*; D015398:Signal Transduction; D051197:Toll-Like Receptor 4 / Q000502:physiology
chemical_list: D015936:Complement C5a; D016207:Cytokines; D008070:Lipopolysaccharides; D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors; D051057:Proto-Oncogene Proteins c-akt; D051197:Toll-Like Receptor 4; C493487:Tlr4 protein, mouse