Platination of superoxide dismutase with cisplatin: tracking the ammonia ligands using Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS).
The high mass accuracy of FT-ICR MS combined with (15)N-labelling shows that mono- and di- platinated products from the reaction of erythrocyte superoxide dismutase with the anticancer drug cisplatin in solution retain their ammine ligands, in contrast to a recent X-ray crystallographic study.
Weidt
Stefan K
SK
School of Chemistry, University of Edinburgh, West Mains Road, Edinburgh, UKEH9 3JJ.
Mackay
C Logan
CL
Langridge-Smith
Pat R R
PR
Sadler
Peter J
PJ
D000641:Ammonia / Q000737:chemistry*; D002945:Cisplatin / Q000737:chemistry*; D007477:Ions / Q000737:chemistry*; D008024:Ligands; D013058:Mass Spectrometry; D008958:Models, Molecular; D017434:Protein Structure, Tertiary; D017550:Spectroscopy, Fourier Transform Infrared; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000378:metabolism*
D007477:Ions; D008024:Ligands; D000641:Ammonia; D013482:Superoxide Dismutase; D002945:Cisplatin