Radiosensitivity of cervical cancer cell lines: the impact of polymorphisms in DNA repair genes.
The aim of this study was to evaluate radiosensitivity of cervical cancer cells in vitro and to assess the relationship between genetic polymorphisms in DNA repair genes and the response of cells to ionizing radiation. The alkaline comet assay as a predictive assay of radiosensitivity was used to examine the susceptibility of four human cervical cancer cell lines (CaSki, C-33A, HeLa and SiHa) to radiation damages. The initial DNA damage and the residual DNA damage at 15, 30, 45 and 60 min after irradiation were assessed. Genotypes of DNA repair genes (XRCC1, hOGG1, PARP, XPD, XRCC3 and XRCC4) were analyzed by PCR-RFLP assays. The comet data clearly indicate a variable but dose-dependent increase in the initial DNA damage in all cell lines. The highest slope of dose response curve was observed in C-33A cells and this cell line was assumed to be radiosensitive. All cell lines repaired DNA damage in a similar manner, the level of DNA strand breakage has returned near the background level within 45 min after irradiation. According to the genotype we found that C-33A cells are polymorphic in the majority of analyzed DNA repair genes. This pilot study indicated associations between polymorphisms in DNA repair genes and cell radiosensitivity.
Gurská
S
S
Laboratory of Mutagenesis and Carcinogenesis, Cancer Research Institute SAS, Bratislava, Slovakia.
Farkasová
T
T
Gábelová
A
A
D020552:Comet Assay; D004249:DNA Damage / Q000528:radiation effects*; D004260:DNA Repair / Q000235:genetics*; D045643:DNA Repair Enzymes / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D010865:Pilot Projects; D011110:Polymorphism, Genetic; D011836:Radiation Tolerance; D011839:Radiation, Ionizing; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000532:radiotherapy
D004268:DNA-Binding Proteins; D045643:DNA Repair Enzymes