[Differentiation of rat mesenchymal stem cells in the microenvironment of choroidal neovascularization].
OBJECTIVE
To assay the differentiation of rat mesenchymal stem cells (MSCs) in the microenvironment of choroidal neovascularization (CNV).


METHODS
Cultured BN rat MSCs were injected into the subretinal space in 25 Argon laser-induced CNV rat. The differentiation of MSCs was traced by immunofluorescent labeling successively in 5 weeks after the surgery.


RESULTS
MSCs distributed on the surface of CNV in the 1st week after injection. From the 2nd week, CD31 positive MSCs were found in CNV until 5 weeks after cell injection, while rhodopsin positive MSCs in the photoreceptor layer were revealed until the 5th week after the injection.


CONCLUSIONS
Injected MSCs in the microenvironment of CNV can differentiate into endothelium and photoreceptor.
Yu
Wei-hong
WH
Department of Ophthalmology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
Dong
Fang-tian
FT
Zhang
Zhi-qiao
ZQ
Huo
Dong-mei
DM
DU
Hong
H
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D020256:Choroidal Neovascularization; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051381:Rats; D011914:Rats, Inbred BN