Binding of lectins to DNA micro-assemblies: modification of nucleo-cages with lactose-conjugated psoralen.
Spherical DNA micro-assemblies appended with lactose units (Lactose-nucleo-cages, Lac-NC) are newly developed. DNA spherical assemblies self-assembled from suitably designed three oligodeoxyribonucleotides (ODNs) 1-3 were cross-linked by lactose-conjugated psoralen derivative 4. Confocal laser scanning fluorescence microscopy (CLSM) observation of Lac-NC shows that rhodamine labeled peanut lectin (Rho-PNA: a galactose-specific lectin) binds to lactose-modified nucleo-cages with higher affinity compared to that of unmodified nucleo-cages. Binding isotherm experiments indicated that the apparent affinity constant of Rho-PNA to Lac-NC was in the order of 10(5) M(-1).
Kim
Kwonil
K
Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
Matsuura
Kazunori
K
Kimizuka
Nobuo
N
D002240:Carbohydrate Sequence; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D011564:Furocoumarins / Q000737:chemistry*; D007785:Lactose / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D019887:Peanut Agglutinin / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D011564:Furocoumarins; D019887:Peanut Agglutinin; D004247:DNA; D007785:Lactose