Genome sequence of a clinical isolate of Campylobacter jejuni from Thailand.
Campylobacter jejuni CG8486, which belongs to the HS4 complex, was isolated from a patient with inflammatory diarrhea in Thailand. This strain caused a diarrheal disease in ferrets comparable to that caused by C. jejuni strain 81-176, but it was much less invasive for epithelial cells in vitro than 81-176. Complete genome sequencing of CG8486 revealed a 1.65-Mb genome that was very similar to the other two published genomes of clinical isolates of C. jejuni, the genomes of 81-176 and NCTC 11168, with a limited number of CG8486-specific genes mapping outside the hypervariable carbohydrate biosynthesis loci. These data suggest that the genes required for induction of inflammatory diarrhea are among the genes shared by CG8486 and 81-176 but that either major changes in the carbohydrate loci and/or more subtle changes in other genes may modulate virulence.
Poly
Frédéric
F
Enteric Diseases Department, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20906, USA.
Read
Timothy
T
Tribble
David R
DR
Baqar
Shahida
S
Lorenzo
Maria
M
Guerry
Patricia
P
D000328:Adult; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D018938:Caco-2 Cells; D002169:Campylobacter Infections / Q000382:microbiology* / Q000503:physiopathology; D016123:Campylobacter jejuni / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D002460:Cell Line; D003967:Diarrhea / Q000382:microbiology* / Q000503:physiopathology; D004195:Disease Models, Animal; D005289:Ferrets; D016680:Genome, Bacterial; D023281:Genomics; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods; D013785:Thailand; D014774:Virulence
D001426:Bacterial Proteins