Effect of zinc on the lipid peroxidation and the antioxidant defense systems of the alloxan-induced diabetic rabbits.
The effects of oral zinc supplementation on lipid peroxidation and the antioxidant defense system of alloxan (80-90 mg/kg)-induced diabetic rabbits were examined. Forty-five New Zealand male rabbits, 1 year old, weighing approximately 2.5 kg, were allocated randomly and equally as control, diabetic, and zinc-supplemented diabetic groups. After diabetes was induced, zinc-supplemented diabetic rabbits had 150 mg/L of zinc as zinc sulfate (ZnSO(4)) in their drinking tap water for 3 months. The feed and water consumption was higher in diabetic groups than (P<0.01) healthy rabbits. The body weight was lower in diabetic rabbits compared to control. The blood glucose levels were higher in diabetic groups than controls. The elevated plasma malondialdehyde (MDA) levels were determined in the diabetic group (P<0.01). The glutathione peroxidase (GSH-Px), catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), and ceruloplasmin levels in the diabetic group were decreased by the effect of diabetes but there was no difference between zinc-supplemented diabetic and control rabbits. Serum zinc concentrations were lower in diabetic rabbits but iron (Fe) and copper (Cu) levels in sera were not different among the groups. As a result, it was concluded that daily zinc supplementation could reduce the harmful effects of oxidative stress in diabetics.
Duzguner
Vesile
V
Department of Biochemistry, Institute of Health Sciences, Mustafa Kemal University, 31034 Hatay, Turkey.
Kaya
Sule
S
D000284:Administration, Oral; D000496:Alloxan / Q000633:toxicity; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001786:Blood Glucose / Q000032:analysis; D002374:Catalase / Q000097:blood / Q000378:metabolism; D002570:Ceruloplasmin / Q000032:analysis / Q000378:metabolism; D003300:Copper / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000378:metabolism*; D005978:Glutathione / Q000097:blood / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000097:blood / Q000378:metabolism; D007501:Iron / Q000097:blood; D015227:Lipid Peroxidation / Q000187:drug effects*; D008315:Malondialdehyde / Q000097:blood; D010088:Oxidoreductases / Q000097:blood / Q000378:metabolism*; D011817:Rabbits; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism; D015032:Zinc / Q000097:blood; D019287:Zinc Sulfate / Q000008:administration & dosage*
D000975:Antioxidants; D001786:Blood Glucose; D008315:Malondialdehyde; D000496:Alloxan; D019287:Zinc Sulfate; D003300:Copper; D007501:Iron; D010088:Oxidoreductases; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D002570:Ceruloplasmin; D005978:Glutathione; D015032:Zinc