title: Expression of adenylate kinase 1 in bovine retinal cytosol.
abstract: Adenylate kinases (AKs) are ubiquitous phosphotransferases that contribute to homeostasis of adenine nucleotide composition in cells. Six AK isoforms were found in vertebrates. We report that soluble AK isoform 1 is expressed in the cytosol of bovine retina consistently devoid of rod outer segments. Immunoblotting analysis with a polyclonal antibody raised against soluble adenylate kinase and subsequent sequencing of eluted peptide by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry allowed enzyme isolation by joining purification methods to two-dimensional electrophoresis. In this study, we found that cytosolic adenylate kinase isoform 1 is expressed in bovine retina. Cytoplasmic AK1 would physiologically contribute to retinal energy metabolism.
authors: Biology Department, University of Genoa, Genova, Italy.
authors: Silvia
authors: S
authors: Ravera
authors: Luca
authors: L
authors: Musante
authors: Daniela
authors: D
authors: Calzia
authors: Isabella
authors: I
authors: Panfoli
authors: Maurizio
authors: M
authors: Bruschi
authors: Giovanni
authors: G
authors: Candiano
authors: Isidoro Mario
authors: IM
authors: Pepe
authors: Alessandro
authors: A
authors: Morelli
mesh_terms: D000263:Adenylate Kinase / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003600:Cytosol / Q000201:enzymology*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D012160:Retina / Q000201:enzymology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D007527:Isoenzymes; D000263:Adenylate Kinase; C110939:adenylate kinase 1