title: Characterization of highly toxic type 2 ribosome-inactivating proteins from Adenia lanceolata and Adenia stenodactyla (Passifloraceae).
abstract: From the caudices of the Passifloraceae Adenia lanceolata and A. stenodactyla, two lectins called lanceolin and stenodactylin, respectively, were purified by affinity chromatography on CL Sepharose 6B. The lectins are glycoproteins with M(r) 61,243 (lanceolin) and 63,131 (stenodactylin), consisting of an enzymatic A chain linked to a larger B chain with lectin properties, with N-terminal amino acid sequences similar to that of volkensin, the toxic lectin from A. volkensii. The lectins agglutinate red blood cells, inhibit protein synthesis both by a cell-free system and by whole cells, and depurinate ribosomes and DNA, but not tRNA or poly(A). They are highly toxic to cells, in which they induce apoptosis, and to mice, with LD(50)s 8.16 microg/kg (lanceolin) and 2.76 microg/kg (stenodactylin) at 48 h. Thus, lanceolin and stenodactylin have all the properties of the toxic type 2 ribosome-inactivating proteins and are amongst the most potent toxins of plant origin.
authors: Dipartimento di Patologia sperimentale, Università di Bologna, I-40126 Bologna, Italy. fiorenzostirpe@libero.it
authors: Fiorenzo
authors: F
authors: Stirpe
authors: Andrea
authors: A
authors: Bolognesi
authors: Massimo
authors: M
authors: Bortolotti
authors: Valentina
authors: V
authors: Farini
authors: Chiara
authors: C
authors: Lubelli
authors: Emanuele
authors: E
authors: Pelosi
authors: Letizia
authors: L
authors: Polito
authors: Barbara
authors: B
authors: Dozza
authors: Paola
authors: P
authors: Strocchi
authors: Angela
authors: A
authors: Chambery
authors: Augusto
authors: A
authors: Parente
authors: Luigi
authors: L
authors: Barbieri
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D004797:Enzyme-Linked Immunosorbent Assay; D006386:Hemagglutination Tests; D006801:Humans; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000633:toxicity*; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009699:N-Glycosyl Hydrolases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000633:toxicity*; D031683:Passifloraceae / Q000201:enzymology*; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000633:toxicity*; D011500:Protein Synthesis Inhibitors / Q000633:toxicity; D011817:Rabbits; D054790:Ribosome Inactivating Proteins, Type 2 / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism* / Q000633:toxicity; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein
chemical_list: D037102:Lectins; D010940:Plant Proteins; D011500:Protein Synthesis Inhibitors; D054790:Ribosome Inactivating Proteins, Type 2; D009699:N-Glycosyl Hydrolases; C523577:lanceolin protein, Adenia lanceolata; C523578:stenodactylin protein, Adenia stenodactyla