title: Epstein-Barr virus and virus human protein interaction maps.
abstract: A comprehensive mapping of interactions among Epstein-Barr virus (EBV) proteins and interactions of EBV proteins with human proteins should provide specific hypotheses and a broad perspective on EBV strategies for replication and persistence. Interactions of EBV proteins with each other and with human proteins were assessed by using a stringent high-throughput yeast two-hybrid system. Overall, 43 interactions between EBV proteins and 173 interactions between EBV and human proteins were identified. EBV-EBV and EBV-human protein interaction, or "interactome" maps provided a framework for hypotheses of protein function. For example, LF2, an EBV protein of unknown function interacted with the EBV immediate early R transactivator (Rta) and was found to inhibit Rta transactivation. From a broader perspective, EBV genes can be divided into two evolutionary classes, "core" genes, which are conserved across all herpesviruses and subfamily specific, or "noncore" genes. Our EBV-EBV interactome map is enriched for interactions among proteins in the same evolutionary class. Furthermore, human proteins targeted by EBV proteins were enriched for highly connected or "hub" proteins and for proteins with relatively short paths to all other proteins in the human interactome network. Targeting of hubs might be an efficient mechanism for EBV reorganization of cellular processes.
authors: Program in Virology, Department of Medicine, The Channing Laboratory, Harvard Medical School and Brigham and Women's Hospital, Boston, MA 02115, USA.
authors: Michael A
authors: MA
authors: Calderwood
authors: Kavitha
authors: K
authors: Venkatesan
authors: Li
authors: L
authors: Xing
authors: Michael R
authors: MR
authors: Chase
authors: Alexei
authors: A
authors: Vazquez
authors: Amy M
authors: AM
authors: Holthaus
authors: Alexandra E
authors: AE
authors: Ewence
authors: Ning
authors: N
authors: Li
authors: Tomoko
authors: T
authors: Hirozane-Kishikawa
authors: David E
authors: DE
authors: Hill
authors: Marc
authors: M
authors: Vidal
authors: Elliott
authors: E
authors: Kieff
authors: Eric
authors: E
authors: Johannsen
mesh_terms: D020031:Epstein-Barr Virus Infections / Q000378:metabolism*; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans; D011401:Promoter Regions, Genetic / Q000235:genetics; D011506:Proteins / Q000378:metabolism*; D013997:Time Factors
chemical_list: D011506:Proteins