Association between fluconazole susceptibility and genetic relatedness among Candida tropicalis isolates in Taiwan.
Among the 162 Candida tropicalis isolates collected in the Taiwan Surveillance of Antimicrobial Resistance of Yeasts in 1999, 23 (14.2 %) had fluconazole MICs > or = 64 mg l(-1), and thus fulfilled the definition of resistance. Random amplified polymorphic DNA assay showed that all 23 fluconazole-resistance C. tropicalis isolates collected from different hospitals around Taiwan were closely related. Two distinct pulsotypes associated with fluconazole susceptibility were identified when these 23 resistant isolates, along with 13 susceptible ones, were analysed by PFGE.
Wang
Jang-Shiun
JS
Division of Clinical Research, National Health Research Institutes, 35 Keyan Road, Zhunan Town, Miaoli County 350, Taiwan, Republic of China.|Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, Republic of China.
Li
Shu-Ying
SY
Division of Laboratory Research and Development, Center for Disease Control, Taipei, Taiwan, Republic of China.
Yang
Yun-Liang
YL
Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan, Republic of China.
Chou
Hsiao-Hui
HH
Division of Laboratory Research and Development, Center for Disease Control, Taipei, Taiwan, Republic of China.
Lo
Hsiu-Jung
HJ
Division of Clinical Research, National Health Research Institutes, 35 Keyan Road, Zhunan Town, Miaoli County 350, Taiwan, Republic of China.
D000935:Antifungal Agents / Q000494:pharmacology; D041022:Candida tropicalis / Q000145:classification* / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D002177:Candidiasis / Q000382:microbiology*; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000235:genetics; D025141:Drug Resistance, Fungal; D016521:Electrophoresis, Gel, Pulsed-Field; D015725:Fluconazole / Q000494:pharmacology*; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D019105:Random Amplified Polymorphic DNA Technique; D013624:Taiwan
D000935:Antifungal Agents; D004271:DNA, Fungal; D015725:Fluconazole