DNA entrapped polypyrrole-polyvinyl sulfonate film for application to electrochemical biosensor.
Double-stranded calf thymus (dsCT)-DNA was electrochemically entrapped into polypyrrole-polyvinyl sulfonate (PPy-PVS) films deposited onto indium tin oxide (ITO) coated glass plates. These dsCT-DNA entrapped PPy-PVS/ITO films were characterized using cyclic voltammetry, UV-visible, Fourier transform infrared (FT-IR), scanning tunneling microscopy (STM), and electrochemical impedance measurements. Attempts made to use these dsCT-DNA entrapped PPy-PVS/ITO films for detection of 2-aminoanthracene (0.001-6.0 ppm) and 3-chlorophenol (0.01-55.0 ppm) revealed a response time of 30s and a shelf life of approximately 25 weeks when stored under desiccated conditions at 25 degrees C. The addition of salts such as Ca(2+) (250 ppm), Mg(2+) (200 ppm), Cl(-) (1560 ppm), and Na(+) (150 ppm) ions contained in water does not affect the observed amperometric response of the disposable dsCT-DNA entrapped PPy-PVS film-based electrochemical biosensor.
Prabhakar
Nirmal
N
Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, Dr. K.S. Krishnan Marg, New Delhi 110012, India.
Arora
Kavita
K
Singh
Surinder P
SP
Singh
Harpal
H
Malhotra
Bansi D
BD
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D004247:DNA; D004563:Electrochemistry; D016252:Microscopy, Scanning Tunneling; D011108:Polymers; D011145:Polyvinyls; D011758:Pyrroles; D017550:Spectroscopy, Fourier Transform Infrared; D017971:Tin Compounds
D011108:Polymers; D011145:Polyvinyls; D011758:Pyrroles; D017971:Tin Compounds; C067635:polypyrrole; C109984:indium tin oxide; D004247:DNA