title: Evaluating the insecticide resistance potential of eight Drosophila melanogaster cytochrome P450 genes by transgenic over-expression.
abstract: In Drosophila melanogaster, the increased expression of Cyp6g1 results in resistance to chemically unrelated insecticides including DDT, neonicotinoids and insect growth regulator insecticides. To determine the insecticide resistance capacity of other D. melanogaster cytochrome P450s, we used the GAL4/UAS system to express individual P450s in the midgut, Malpighian tubules and fat body of transgenic flies. Drosophila over-expressing Cyp6g1, Cyp6g2, Cyp6t3, Cyp6a2, Cyp6a8, Cyp6a19, Cyp6a23 and Cyp12d1 were screened for resistance to four insecticides--DDT, nitenpyram, dicyclanil and diazinon. Increased survival on insecticides is detected for Cyp6g1 (DDT, nitenpyram and dicyclanil), Cyp6g2 (nitenpyram and diazinon) and Cyp12d1 (DDT and dicyclanil) over-expression lines. No increased survival on any insecticide was detected for flies over-expressing either Cyp6a2, Cyp6a8, Cyp6t3, Cyp6a19 or Cyp6a23.
authors: Centre for Environmental Stress and Adaptation Research (CESAR), Department of Genetics, Bio21 Molecular and Biotechnology Institute, The University of Melbourne, Vic. 3010, Australia.
authors: Phillip J
authors: PJ
authors: Daborn
authors: Christopher
authors: C
authors: Lumb
authors: Adrian
authors: A
authors: Boey
authors: Wayn
authors: W
authors: Wong
authors: Richard H
authors: RH
authors: Ffrench-Constant
authors: Philip
authors: P
authors: Batterham
mesh_terms: D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics*; D007305:Insecticide Resistance / Q000235:genetics; D030781:Organisms, Genetically Modified / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D003577:Cytochrome P-450 Enzyme System