title: Systems biology of innate immunity.
abstract: Systems Biology has emerged as an exciting research approach in molecular biology and functional genomics that involves a systematic use of genomic, proteomic, and metabolomic technologies for the construction of network-based models of biological processes. These endeavors, collectively referred to as systems biology establish a paradigm by which to systematically interrogate, model, and iteratively refine our knowledge of the regulatory events within a cell. Here, we present a new systems approach, integrating DNA and transcript expression information, specifically designed to identify transcriptional networks governing the macrophage immune response to lipopolysaccharide (LPS). Using this approach, we are not only able to infer a global macrophage transcriptional network, but also time-specific sub-networks that are dynamically active across the LPS response. We believe that our system biological approach could be useful for identifying other complex networks mediating immunological responses.
authors: Unit of Computational Medicine, King Gustaf V Research Institute, Department of Medicine, Karolinska Institute, SE-171 76 Stockholm, Sweden. jespert@ifm.liu.se
authors: Jesper
authors: J
authors: Tegnér
authors: Roland
authors: R
authors: Nilsson
authors: Vladimir B
authors: VB
authors: Bajic
authors: Johan
authors: J
authors: Björkegren
authors: Timothy
authors: T
authors: Ravasi
mesh_terms: D006801:Humans; D007113:Immunity, Innate / Q000235:genetics / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D018448:Models, Immunological; D020411:Oligonucleotide Array Sequence Analysis; D049490:Systems Biology; D014158:Transcription, Genetic
chemical_list: D008070:Lipopolysaccharides