Bovine platelet-activating factor acetylhydrolase (PAF-AH) activity related to fertility.
Plasma platelet-activating factor acetylhydrolase (PAF-AH), the enzyme characterized by the association with plasma lipoproteins, degrades platelet-activating factor (PAF) as well as PAF-like oxidatively fragmented phospholipids produced during oxidative stress. Apart from pro-inflammatory properties, PAF is also related to reproductive processes and successful fertility. In order to get a better insight into the involvement of PAF-AH in the fertility of cows, the aim of the study was to determine the PAF-AH activity as well as the C-reactive protein, cholesterol and high density lipoprotein-cholesterol (HDL-C) in the serum of dairy cows throughout the pregnancy and lactation, as well as in infertile cows. The results showed that serum PAF-AH activity changes throughout pregnancy and lactation with a lower level during periparturient period. It is also found higher PAF-AH activity in lactating cows with reproductive disorders compared to high lactating cows without reproductive disorders. Strong correlation between PAF-AH activity and HDL-C concentration indicates that HDL could have considerable influence on PAF-AH activity in bovine plasma. CRP concentration was also lower during transition period suggesting that lactation might stimulate CRP synthesis in bovine. A higher CRP concentration in cows with reproductive disorders compared to fertile cows at the peak of lactation, demonstrates that milk production is not the only factor influencing CRP in cows. A significant correlation between PAF-AH activity and CRP level shows that both parameters could be influenced by reproductive status of dairy cows.
Turk
Romana
R
Department of Pathophysiology, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, Zagreb, Croatia. rturk@vef.hr
Juretić
Dubravka
D
Geres
Darko
D
Bacić
Goran
G
Milesević
Mario
M
Flegar-Mestrić
Zlata
Z
Turk
Nenad
N
Svetina
Ante
A
D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase / Q000097:blood*; D000818:Animals; D002097:C-Reactive Protein / Q000378:metabolism; D002417:Cattle / Q000097:blood / Q000502:physiology*; D008076:Cholesterol, HDL / Q000097:blood; D005260:Female; D005298:Fertility / Q000502:physiology*; D007774:Lactation; D011247:Pregnancy; D018709:Statistics, Nonparametric
D008076:Cholesterol, HDL; D002097:C-Reactive Protein; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase