title: The impact of acrylonitrile and bioaugmentation on the biodegradation activity and bacterial community structure of a topsoil.
abstract: The analysis of the bacterial community within the soil using DGGE showed acrylonitrile (ACN) could lead to the selection of significantly similar communities. Moreover, Rhodococcus sp. AJ270 was successfully established in the soil community. High GC G+-bacteria also responded positively to ACN addition. Bioaugmentation or carbon addition had no impact on the rate or degree of ACN degradation. ACN could be readily degraded by the soil bacteria, however, the community structure was significantly affected by its addition as well as by the addition of carbon or Rhodococcus sp. AJ270. The bioaugmentation of the soil with this strain was successful, in that the organism became established within the community. ACN addition to a soil produces statistically significant changes in the bacterial community.
authors: Biomolecular and Biomedical Research Centre, School of Applied Sciences, Northumbria University, Newcastle-upon-Tyne, UK.
authors: J
authors: J
authors: Baxter
authors: N J
authors: NJ
authors: Garton
authors: S P
authors: SP
authors: Cummings
mesh_terms: D000181:Acrylonitrile / Q000378:metabolism*; D001419:Bacteria / Q000145:classification / Q000254:growth & development* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002244:Carbon / Q000378:metabolism; D015169:Colony Count, Microbial; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D009691:Nucleic Acid Denaturation; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D002244:Carbon; D000181:Acrylonitrile