Enhancement of salivary IgA response to a DNA vaccine against Streptococcus mutans wall-associated protein A in mice by plasmid-based adjuvants.
A specific salivary IgA (sIgA) response was obtained in mice by intranasal immunization with a naked DNA vaccine consisting of the Streptococcus mutans wall-associated protein A gene (wapA) inserted into the mammalian expression vector pcDNA3.1/V5/His-TOPO. In the present study, the vaccine, referred to as pcDNA-wapA, was administered with or without the cationic lipid DMRIE-C. No mucosal response was observed in mice immunized with the vaccine alone, whereas a weak and temporal sIgA response was obtained when the vaccine was mixed with DMRIE-C. To investigate the use of pcDNA containing the interleukin 5 (IL-5) gene (pcDNA-il-5) or the cholera toxin B gene (pcDNA-ctb) as genetic adjuvants, these constructs were used in co-immunization studies. The enhancement effect was transient with pcDNA-il-5, but longer lasting with pcDNA-ctb, thus supporting the use of the latter as a genetic adjuvant to DNA vaccine.
Han
Thomas K
TK
Department of Biology, University of South Florida, Tampa, FL, USA.
Dao
My Lien
ML
Department of Biology, University of South Florida, Tampa, FL, USA.
D000276:Adjuvants, Immunologic / Q000235:genetics; D000281:Administration, Intranasal; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000276:immunology; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D002772:Cholera Toxin / Q000235:genetics / Q000276:immunology; D005260:Female; D005822:Genetic Vectors; D018928:Immunity, Mucosal; D007071:Immunoglobulin A, Secretory / Q000032:analysis* / Q000276:immunology; D015848:Interleukin-5 / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics; D012463:Saliva / Q000276:immunology*; D022541:Streptococcal Vaccines / Q000235:genetics / Q000276:immunology*; D013295:Streptococcus mutans / Q000276:immunology*; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007071:Immunoglobulin A, Secretory; D015848:Interleukin-5; D022541:Streptococcal Vaccines; D019444:Vaccines, DNA; C081813:wapA protein, Bacillus subtilis; D002772:Cholera Toxin