Protection of yeast lacking the Ure2 protein against the toxicity of heavy metals and hydroperoxides by antioxidants.
The aim of this study was to examine the protection of the yeast lacking the "antioxidant-like" prion precursor protein (Ure2p), by antioxidants and to elucidate how modification of redox homeostasis affects toxicity of agents inducing oxidative stress in the Deltaure2 cells. We found a diverse ability of a range of antioxidants to ameliorate the hypersensitivity of the Deltaure2 disruptant to oxidants and heavy metal ions. Glutathione and then ascorbate were the most effective antioxidants; Tempol, Trolox and melatonin were much less effective or even hampered the growth of the Deltaure2 cells exposed to tested agents. The intracellular level of ROS was augmented in the Deltaure2 mutant under normal growth conditions (1.7-fold), and after treatment with H(2)O(2) (2.3-fold) and Cd(II) (2.8-fold), with respect to its wild-type counterpart. Glutathione was unable to prevent the increase in ROS production caused by CdCl(2). The Deltaure2 disruptant was also hypersensitive to heat shock, like mutants lacking glutathione S-transferases.
Lewinska
Anna
A
Department of Biochemistry and Cell Biology, University of Rzeszow, Rzeszow, Poland. alewinska@o2.pl
Bartosz
Grzegorz
G
D000975:Antioxidants / Q000494:pharmacology*; D001555:Benzene Derivatives / Q000633:toxicity*; D002104:Cadmium / Q000633:toxicity*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase / Q000378:metabolism; D006358:Hot Temperature; D009154:Mutation / Q000235:genetics; D016877:Oxidants / Q000633:toxicity*; D018384:Oxidative Stress; D011328:Prions / Q000235:genetics / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
D000975:Antioxidants; D001555:Benzene Derivatives; D016877:Oxidants; D011328:Prions; D017382:Reactive Oxygen Species; D029701:Saccharomyces cerevisiae Proteins; D002104:Cadmium; D005979:Glutathione Peroxidase; C067020:URE2 protein, S cerevisiae; D005982:Glutathione Transferase; D005978:Glutathione; C007164:cumene hydroperoxide