Intragenomic diversity of the V1 regions of 16S rRNA genes in high-alkaline protease-producing Bacillus clausii spp.
Alkaliphilic Bacillus sp. strain KSM-K16, which produces high-alkaline M-protease, was characterized phenotypically, biochemically and genetically. This strain was identified as Bacillus clausii based on the results of taxonomic studies, including sequencing of the 16S rRNA gene and DNA-DNA hybridization. Seven rRNA operons in the genome were identified by pulsed-field gel electrophoresis. Sequencing of cloned 16S rRNA genes revealed two distinct types of variable region V1. Moreover, some cloned 16S rRNA genes in some of the reference strains of B. clausii had a V1 region of yet another type. The B. clausii strains could clearly be divided into at least two subgroups based on the frequencies of the types of cloned V1 sequence. Bacillus sp. strain KSM-K16 was found to be in a different phylogenetic position from other high-alkaline protease-producing strains of B. clausii.
Kageyama
Yasushi
Y
Tochigi Research Laboratories of Kao Corporation, 2606 Akabane, Ichikai, Haga, Tochigi 321-3497, Japan. kageyama.yasushi@kao.co.jp
Takaki
Yoshihiro
Y
Shimamura
Shigeru
S
Nishi
Shinro
S
Nogi
Yuichi
Y
Uchimura
Kohsuke
K
Kobayashi
Tohru
T
Hitomi
Jun
J
Ozaki
Katsuya
K
Kawai
Shuji
S
Ito
Susumu
S
Horikoshi
Koki
K
D001407:Bacillus / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis*; D004275:DNA, Ribosomal / Q000032:analysis*; D016521:Electrophoresis, Gel, Pulsed-Field; D014644:Genetic Variation; D005838:Genotype; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009876:Operon; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D021521:Ribotyping; D017422:Sequence Analysis, DNA; D012697:Serine Endopeptidases / Q000378:metabolism*; D013381:Subtilisins / Q000378:metabolism*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; C108281:M-protease; D012697:Serine Endopeptidases; D013381:Subtilisins; C032923:microbial serine proteinases