[Orthogonal experiment using SFE-CO2 in extraction of essential oil from fresh Houttuynia cordata and analysis of essential oil by GC-MS].
OBJECTIVE
To optimize the extraction procedure of essential oil from H. cordata using the SFE-CO2 and analyze the chemical composition of the essential oil.


METHODS
The extraction procedure of essential oil from fresh H. cordata was optimized with the orthogonal experiment. Essential oil of fresh H. cordata was analysed by GC-MS.


RESULTS
The optimize preparative procedure was as follow: essential oil of H. cordata was extracted at a temperature of 35 degrees C, pressure of 15,000 kPa for 20 min. 38 chemical components were identified and the relative contents were quantified.


CONCLUSIONS
The optimum preparative procedure is reliable and can guarantee the quality of essential oil.
Meng
Jiang
J
School of Chinese Medicine of Hong Kong Baptist University, Hong Kong, China.
Dong
Xiao-ping
XP
Zhou
Yi-sheng
YS
Jiang
Zhi-hong
ZH
Leung
Kelvin Sze-Yin
KS
Zhao
Zhong-zhen
ZZ
D000447:Aldehydes / Q000032:analysis / Q000737:chemistry; D002245:Carbon Dioxide / Q000737:chemistry; D025924:Chromatography, Supercritical Fluid / Q000379:methods; D005612:Freeze Drying; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D052859:Houttuynia / Q000737:chemistry*; D007659:Ketones / Q000032:analysis* / Q000737:chemistry; D009822:Oils, Volatile / Q000032:analysis* / Q000737:chemistry; D035261:Plant Components, Aerial / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011312:Pressure; D013696:Temperature
D000447:Aldehydes; D007659:Ketones; D009822:Oils, Volatile; D002245:Carbon Dioxide; C023969:decanoyl acetaldehyde; C526928:undecan-2-one