title: Galectin-1 mediated suppression of Epstein-Barr virus specific T-cell immunity in classic Hodgkin lymphoma.
abstract: In Hodgkin lymphoma (HL), the malignant Hodgkin Reed-Sternberg cells interact with the host microenvironment to create an immunosuppressive network that protects the lymphoma from immune attack. These mechanisms are not fully understood. We examined the role of the immunomodulatory protein galectin-1 (Gal-1) on Epstein-Barr virus-specific CD8(+) T cell responses in HL. Initial studies indicated Gal-1 expression in all in vitro established Hodgkin Reed-Sternberg cell lines. In situ analysis revealed Gal-1 expression in 26 of 42 classic HL, whereas Gal-1 was uniformly negative in nodular lymphocyte predominant HL. Gal-1(hi) expression was associated with male gender, older patients, reduced CD8(+) T cell infiltration at the tumor site, and most importantly, an impaired latent membrane protein 1 and 2-specific CD8(+) T-cell responses. In vitro exposure to recombinant Gal-1 inhibited proliferation and interferon-gamma expression by Epstein-Barr virus-specific T cells. These observations provide an important link between the Gal-1-mediated immunomodulatory networks and loss of antigen-specific T-cell function in classic HL.
authors: Australian Centre for Vaccine Development, Tumor Immunology Laboratory, Division of Infectious Diseases and Immunology, Queensland Institute of Medical Research, Brisbane, Australia.
authors: Maher K
authors: MK
authors: Gandhi
authors: Guido
authors: G
authors: Moll
authors: Corey
authors: C
authors: Smith
authors: Ujjwal
authors: U
authors: Dua
authors: Eleanore
authors: E
authors: Lambley
authors: Olivier
authors: O
authors: Ramuz
authors: Devinder
authors: D
authors: Gill
authors: Paula
authors: P
authors: Marlton
authors: John F
authors: JF
authors: Seymour
authors: Rajiv
authors: R
authors: Khanna
mesh_terms: D000328:Adult; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D049109:Cell Proliferation; D020031:Epstein-Barr Virus Infections / Q000235:genetics / Q000378:metabolism / Q000821:virology; D005260:Female; D037483:Galectin 1 / Q000378:metabolism*; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000378:metabolism*; D006689:Hodgkin Disease / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D016246:Lymphocytes, Tumor-Infiltrating / Q000378:metabolism*; D008297:Male; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D016539:Reed-Sternberg Cells / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes
chemical_list: D037483:Galectin 1; D011994:Recombinant Proteins; D007371:Interferon-gamma