Cold-active acetogenic bacteria from surficial sediments of perennially ice-covered Lake Fryxell, Antarctica.
Cold-active acetogenic bacteria in the permanently cold sediments of Lake Fryxell, Antarctica were investigated using culture-based methods. Two psychrophilic, acetogenic strains were isolated and found to be physiologically and phylogenetically related to Acetobacterium bakii and Acetobacterium tundrae. However, the Antarctic isolates showed a lower growth temperature range than other species of Acetobacterium, with growth occurring from -2.5 to 25 degrees C and optimally at 19-21 degrees C. Cultures incubated at +5 and +1 degrees C grew with generation times of 7 and 9 days, respectively. The rapid growth of these strains at low temperatures suggests that acetogenesis may be an important anaerobic process in the sediments of Lake Fryxell.
Sattley
W Matthew
WM
Department of Microbiology, Southern Illinois University, Carbondale, IL 62901, USA.
Madigan
Michael T
MT
D019342:Acetic Acid / Q000378:metabolism; D045855:Acetobacterium / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism / Q000648:ultrastructure; D000864:Antarctic Regions; D015373:Bacterial Typing Techniques; D003080:Cold Temperature; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005618:Fresh Water / Q000382:microbiology*; D019015:Geologic Sediments / Q000382:microbiology*; D046448:Ice Cover; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D019342:Acetic Acid