Wen-pi-tang-Hab-Wu-ling-san attenuates kidney ischemia/reperfusion injury in mice. A role for antioxidant enzymes and heat-shock proteins.
The purpose of this study was to investigate the effects of Wen-pi-tang-Hab-Wu-ling-san (WHW) extract, which has been used for treatment of renal diseases, on kidney ischemia/reperfusion (I/R) injury. Thirty minutes of bilateral renal ischemia resulted in disruption of kidney tubular epithelial cells and increased plasma creatinine levels in mice, however these effects were significantly attenuated when WHW was administered prior to I/R. WHW-administration also inhibited post-ischemic decreases of catalase, copper-zinc superoxide dismutase (CuZnSOD), and manganese superoxide dismutase (MnSOD) activity in kidney tissue, leading to decreased tissue hydrogen peroxide levels and lipid peroxidation. Post-ischemic increases of heat-shock protein (HSP)-27 and -72 expressions were greater in mouse kidneys that received WHW. In conclusion, WHW-administration reduced kidney susceptibility to I/R injury, and this reduced susceptibility was associated with greater post-ischemic activation of catalase, CuZnSOD and MnSOD, resulting in reduced hydrogen peroxide levels and lipid peroxidation, as well as higher post-ischemic expression of HSP-27 and -72.
Seok
Young Mi
YM
Department of Anatomy, Kyungpook National University School of Medicine, 101 Dongin2ga, Junggu, Daegu 700-422, South Korea.
Kim
Jinu
J
Choi
Ki Choon
KC
Yoon
Cheol Ho
CH
Boo
Yong Chool
YC
Park
Yongki
Y
Park
Kwon Moo
KM
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D015153:Blotting, Western; D002374:Catalase / Q000378:metabolism; D003600:Cytosol / Q000187:drug effects / Q000201:enzymology; D004365:Drugs, Chinese Herbal / Q000378:metabolism*; D050884:HSP72 Heat-Shock Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D007668:Kidney / Q000473:pathology; D007674:Kidney Diseases / Q000188:drug therapy* / Q000201:enzymology* / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000201:enzymology* / Q000473:pathology; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C521629:Wen-pi-tang-Hab-Wu-ling-san; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase