Statistical optimization of culture conditions for 1,3-propanediol by Klebsiella pneumoniae AC 15 via central composite design.
A central composite design was used to study the effect of glycerol, rate of stirring, air aeration and pH on the synthesis of 1,3-propanediol (1,3-PD) by Klebsiella pneumoniae AC 15. Among the four variables, glycerol and rate of stirring significantly affected 1,3-PD productivity, whereas air aeration and pH were not effective. A quadratic polynomial equation was obtained for 1,3-PD productivity by multiple regression analysis using response surface methodology. The validation experimental confirmed with the predicted model. The optimum combinations for 1,3-PD productivity was glycerol, rate of stirring, air aeration, and pH of 50 g/l, 318 rpm, 0.6 vvm, 6.48, respectively. The subsequent fed batch experiments produced 1,3-PD of 70 g/l at a fermentation of 30 h.
Zheng
Zong-ming
ZM
Department of Chemistry Engineering, Tsinghua University, 100084 Beijing, China.
Hu
Qiu-long
QL
Hao
Jian
J
Xu
Feng
F
Guo
Ni-ni
NN
Sun
Yan
Y
Liu
De-hua
DH
D000388:Air; D001431:Bacteriological Techniques; D019149:Bioreactors / Q000382:microbiology; D003470:Culture Media; D005990:Glycerol / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007711:Klebsiella pneumoniae / Q000145:classification / Q000378:metabolism*; D011409:Propylene Glycols / Q000378:metabolism*; D013997:Time Factors
D003470:Culture Media; D011409:Propylene Glycols; C041787:1,3-propanediol; D005990:Glycerol