title: Molecular cloning and characterization of a novel splice variant of the LIM domain family gene, PINCH 2, in human testis.
abstract: By hybridizing human adult testis cDNA microarrays with human adult and embryo testis cDNA probes, we identified a novel human testis gene, PINCH 2. PINCH 2 expression was 3.4-fold higher in adult than in fetal testis. The full length of its cDNA was 963 bp, with a 354-bp open reading frame (ORF), encoding a 117-amino acid protein. PINCH 2 was a splicing isoform of PINCH. It shared one exon, which encoded the LIM domain, with PINCH gene in human genome. Multitissue reverse transcriptase-polymerase chain reaction (RTPCR) analysis revealed that this gene was expressed variably in a wide range of tissues, with high expression levels in human adult testis. These results suggest that PINCH 2, a novel LIM domain-containing gene, may play an important role in testicular development/spermatogenesis.
authors: Institute of Stomatology, Nanjing Medical University, 140 Han Zhong Road, Nanjing 210029, People's Republic of China.
authors: Yun
authors: Y
authors: Liu
authors: Jin
authors: J
authors: Liu
authors: Jie
authors: J
authors: Chen
authors: Libo
authors: L
authors: Cheng
authors: Qinhong
authors: Q
authors: Cao
authors: Li
authors: L
authors: Zhu
authors: Yan
authors: Y
authors: Sun
authors: Qinghuai
authors: Q
authors: Liu
authors: Jianmin
authors: J
authors: Li
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D001709:Biotechnology; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D014644:Genetic Variation; D006801:Humans; D060588:LIM Domain Proteins; D008297:Male; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D005810:Multigene Family; D020411:Oligonucleotide Array Sequence Analysis; D011247:Pregnancy; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013091:Spermatogenesis / Q000235:genetics; D013737:Testis / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D017931:DNA Primers; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D060588:LIM Domain Proteins; C490235:LIMS2 protein, human; D008565:Membrane Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger