Preparation of cultured myofibers from larval salamander limbs for cellular plasticity studies.
Identification of the mechanisms underlying cellular plasticity in salamander cells is important because these may give pointers to the restricted regenerative ability of mammals. The myofibers from salamanders are remarkable for their ability to undergo cellularization and cell-cycle re-entry during regeneration. Here, we describe a detailed method for the isolation and culture of larval salamander myofibers in numbers suitable for cellular plasticity studies. The basic protocol for isolation and purification of cells can be completed in 4 h.
Kumar
Anoop
A
Department of Biochemistry and Molecular Biology, University College London, Gower Street, London WC1E 6BT, UK.
Brockes
Jeremy P
JP
D000558:Ambystoma mexicanum; D000818:Animals; D005121:Extremities / Q000033:anatomy & histology; D016591:Histocytological Preparation Techniques / Q000379:methods*; D007814:Larva / Q000166:cytology; D018485:Muscle Fibers, Skeletal / Q000166:cytology* / Q000502:physiology; D018995:Myosin Heavy Chains / Q000032:analysis; D012333:RNA, Messenger / Q000378:metabolism; D012038:Regeneration; D016707:Tissue Fixation
D012333:RNA, Messenger; D018995:Myosin Heavy Chains