Attachment and phospholipase A2-induced lysis of phospholipid bilayer vesicles to plasma-polymerized maleic anhydride/SiO2 multilayers.
This article describes a method by which intact vesicles can be chemically attached to hydrolyzed maleic anhydride films covalently bound to plasma-polymerized SiO2 on Au substrates. Surface plasmon field-enhanced fluorescence spectroscopy (SPFS) combined with surface plasmon resonance spectroscopy (SPR) was used to monitor the activation of plasma-deposited maleic anhydride (pp-MA) film with EDC/NHS and the subsequent coupling of lipid vesicles. The vesicles were formed from a mixture of phosphatidylcholine and phosphatidylethanolamine lipids, with a water-soluble fluorophore encapsulated within. Vesicle attachment was measured in real time on plasma films formed under different pulse conditions (plasma duty cycle). Optimum vesicle attachment was observed on the pp-MA films containing the highest density of maleic anhydride groups. Phospholipase A2 was used to lyse the surface-bound vesicles and to release the encapsulated fluorophore.
Chifen
Anye N
AN
Max-Planck-Institute für Polymerforschung, Ackermannweg 10, 55128 Mainz, Germany.
Förch
Renate
R
Knoll
Wolfgang
W
Cameron
Petra J
PJ
Khor
Hwei L
HL
Williams
Thomas L
TL
Jenkins
A Toby A
AT
D000818:Animals; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D005456:Fluorescent Dyes; D006046:Gold; D066298:In Vitro Techniques; D008051:Lipid Bilayers / Q000737:chemistry*; D008299:Maleic Anhydrides; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010741:Phospholipases A; D054467:Phospholipases A2; D012822:Silicon Dioxide; D013050:Spectrometry, Fluorescence; D020349:Surface Plasmon Resonance
D020099:Coated Materials, Biocompatible; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D008299:Maleic Anhydrides; D010714:Phosphatidylethanolamines; D006046:Gold; D012822:Silicon Dioxide; D010741:Phospholipases A; D054467:Phospholipases A2; D004134:Dimyristoylphosphatidylcholine; C018525:1,2-dimyristoylphosphatidylethanolamine