title: Pathogenic chaperone-like RNA induces congophilic aggregates and facilitates neurodegeneration in Drosophila.
abstract: Protein aggregation is a hallmark of many neurodegenerative diseases. RNA chaperones have been suggested to play a role in protein misfolding and aggregation. Noncoding, highly structured RNA recently has been demonstrated to facilitate transformation of recombinant and cellular prion protein into proteinase K-resistant, congophilic, insoluble aggregates and to generate cytotoxic oligomers in vitro. Transgenic Drosophila melanogaster strains were developed to express highly structured RNA under control of a heat shock promoter. Expression of a specific construct strongly perturbed fly behavior, caused significant decline in learning and memory retention of adult males, and was coincident with the formation of intracellular congophilic aggregates in the brain and other tissues of adult and larval stages. Additionally, neuronal cell pathology of adult flies was similar to that observed in human Parkinson's and Alzheimer's disease. This novel model demonstrates that expression of a specific highly structured RNA alone is sufficient to trigger neurodegeneration, possibly through chaperone-like facilitation of protein misfolding and aggregation.
authors: Pavlov Institute of Physiology, St. Petersburg 199034, Russia.
authors: Elena
authors: E
authors: Savvateeva-Popova
authors: Andrei
authors: A
authors: Popov
authors: Abraham
authors: A
authors: Grossman
authors: Ekaterina
authors: E
authors: Nikitina
authors: Anna
authors: A
authors: Medvedeva
authors: Alexander
authors: A
authors: Peresleni
authors: Leonid
authors: L
authors: Korochkin
authors: James G
authors: JG
authors: Moe
authors: Eliot
authors: E
authors: Davidowitz
authors: Konstantin
authors: K
authors: Pyatkov
authors: Elena
authors: E
authors: Myasnyankina
authors: Olga
authors: O
authors: Zatsepina
authors: Natalia
authors: N
authors: Schostak
authors: Elena
authors: E
authors: Zelentsova
authors: Michael
authors: M
authors: Evgen'ev
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001921:Brain / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D002470:Cell Survival; D003224:Congo Red / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D018869:Heat-Shock Response; D007814:Larva / Q000166:cytology; D008136:Longevity; D008297:Male; D008568:Memory; D018832:Molecular Chaperones / Q000378:metabolism*; D009410:Nerve Degeneration / Q000473:pathology*; D020836:Protein Structure, Quaternary; D022661:RNA, Untranslated / Q000378:metabolism*; D012469:Salivary Glands / Q000166:cytology / Q000378:metabolism / Q000473:pathology
chemical_list: D018832:Molecular Chaperones; D022661:RNA, Untranslated; D003224:Congo Red