Direct electrochemistry of hemoglobin in the hyaluronic acid films.
Hemoglobin (Hb) in the hyaluronic acid (HA) was cast at pyrolytic graphite (PG) electrodes for researching its electrochemical and electrocatalytic properties. The formal potential and electron transfer rate constant of Hb on HA films were determined, and the stability of the films, the pH effect, and the influence of supporting electrolyte concentrations upon Hb electrochemistry on the films were investigated by cyclic voltammetry and square wave voltammetry. UV-Vis absorption and reflectance absorption infrared (RAIR) spectra showed that the protein on HA film retained near-native secondary structure. The stable Hb-HA/PG gave analytically useful electrochemical catalytic responses to hydrogen peroxide. Thus, the property of the HA film for sorption and retention of water maybe utilized to develop some new biosensors.
Ma
Ling
L
Institute of Molecular Science, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, 030006, PR China.
Tian
Yanni
Y
Rong
Zhijiang
Z
D000818:Animals; D002417:Cattle; D004187:Disaccharides / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods; D006454:Hemoglobins / Q000737:chemistry*; D006820:Hyaluronic Acid / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D013055:Spectrophotometry, Infrared
D004187:Disaccharides; D006454:Hemoglobins; D006820:Hyaluronic Acid