Cloning and sequencing of the class A beta-lactamase gene (blaTEM-15) located on a chromosomal Tn801 transposon.
Escherichia coli CA0210 was identified in a stool culture of a 03-month-neonate in Tunisia. This strain was resistant to beta-lactams, including ureidopenicillins, ticarcillin-clavulanic acid, cefpirome, ceftazidime, and cefotaxime, but it remained susceptible to imipenem and cefoxitin. The beta-lactam-hydrolyzing beta-lactamase gene of E. coli CA0210 and the upstream and downstream regions were cloned, sequenced, and expressed in E. coli DH5alpha. These resistances were carried by a 1080-bp chromosomal gene that encoded a beta-lactamase with a pI of 6.3. Cloning and sequencing experiments showed that the corresponding blaTEM-15 gene was part of a chromosomally located Tn801 transposon.
Chouchani
Chedly
C
Laboratoire de Biochimie et de Biotechnologie, Faculté des Sciences de Tunis, 2092 El-Manar II, Tunisia. chdoula77@yahoo.fr
Ben-Achour
Nahed
N
M'Charek
Arij
A
Belhadj
Omrane
O
D001483:Base Sequence; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics; D005243:Feces / Q000382:microbiology; D006801:Humans; D007223:Infant; D007526:Isoelectric Point; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000737:chemistry / Q000235:genetics*; D047090:beta-Lactams / Q000494:pharmacology
D004251:DNA Transposable Elements; D029968:Escherichia coli Proteins; D047090:beta-Lactams; C106857:TEM-15 beta-lactamase; D001618:beta-Lactamases