Multiplicity editing including quaternary carbons: improved performance for the 13C-DEPTQ pulse sequence.
An improved version of the DEPTQ experiment yielding the signal and multiplicity information for all carbon types including the signals of quaternary carbons is proposed. It encompasses all the known advantages of the basic DEPT experiment. In comparison to the original version, signals of the sensitivity-limiting quaternary carbons are markedly increased: the initial 13C pulse may be adjusted to the Ernst angle, the NOE build-up period is prolonged by the split relaxation delay and a partial recovery of signal losses due to instrumental imperfections is achieved by the incorporation of composite adiabatic 13C refocussing pulses. Furthermore, pure absorption lineshapes for all carbon types are obtained with only one single scan. These attributes make this experiment attractive for 13C analysis of small molecules (including spectral editing), particularly in high-throughput analysis laboratories.
Bigler
Peter
P
Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, CH-3012 Berne, Switzerland. bigler@ioc.unibe.ch
Kümmerle
Rainer
R
Bermel
Wolfgang
W
D002244:Carbon / Q000737:chemistry*; D002247:Carbon Isotopes; D007052:Ibuprofen; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011422:Propionates / Q000737:chemistry; D012680:Sensitivity and Specificity
D002247:Carbon Isotopes; D011422:Propionates; D002244:Carbon; D007052:Ibuprofen