Establishment of a cottontail rabbit papillomavirus/HLA-A2.1 transgenic rabbit model.
Three transgenic rabbit lines that express a well-characterized human major histocompatibility complex class I (MHC-I) gene (HLA-A2.1) have been established. All three lines carry the HLA-A2.1 heavy chain and are able to pass the transgene to their offspring with both the outbred and the inbred EIII/JC genetic background. HLA-A2.1 colocalizes exclusively with rabbit MHC-I on the cell surfaces. These HLA-A2.1 transgenic rabbits demonstrated infection patterns similar to those found after cottontail rabbit papillomavirus (CRPV) challenge when compared with results in normal rabbits, although higher regression rates were found in HLA-A2.1 transgenic rabbits. Because the CRPV genome can accommodate significant modifications, the CRPV/HLA-A2.1 rabbit model has the potential to be used to screen HLA-A2.1-restricted immunogenic epitopes from human papillomaviruses in the context of in vivo papillomavirus infection.
Hu
Jiafen
J
Jake Gittlen Cancer Research Foundation, 500 University Dr., Pennsylvania State University, Hershey, PA 17033, USA.
Peng
Xuwen
X
Budgeon
Lynn R
LR
Cladel
Nancy M
NM
Balogh
Karla K
KK
Christensen
Neil D
ND
D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D012777:Cottontail rabbit papillomavirus / Q000276:immunology*; D004195:Disease Models, Animal; D015789:HLA-A2 Antigen / Q000235:genetics / Q000276:immunology*; D006801:Humans; D030361:Papillomavirus Infections / Q000235:genetics / Q000276:immunology*; D011817:Rabbits
D015789:HLA-A2 Antigen