[Study on the structure and function of a stable methane-oxidizing mixed microbial consortium].
From an agricultural sample taken in Chongqing, a stable methane-oxidizing mixed microbial consortium was established by enrichment culture with methane as a sole source of carbon and energy. The mixed consortium showed high capability of phenol degradation and 1,2-epoxypropane production from propene. More than 99% of phenol at an initial concentration of 600mg/L could be degraded by the mixed microbial consortium after 11 h of cultivation. The productivity of 1, 2-epoxypropane could be increased with the decrease of phosphate concentration. The concentration of 1, 2-epoxypropane produced could reach to 5.0mmol/L. The bacterial structure of the methane-oxidizing mixed microbial consortium was analyzed by pure culture isolation combining with 16S rRNA and PCR of the related MMO functional genes. The results showed that the methane-oxidizing mixed microbial consortium was composed of a type 1U methanotroph identified as Methylosinus trichosporium and at least 4 kinds of heterotrophs ( Comamonas testosteroni, Cupriavidus metallidurans, Acinetobacter junii and Stenotrophomonas maltophilia ). M. trichosporium Y9, isolated from the mixed consortium, harbored both sMMO and pMMO genes.
Luo
Ming-fang
MF
Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China. luomf@gucas.ac.cn
Wu
Hao
H
Wang
Lei
L
Xing
Xin-hui
XH
D001419:Bacteria / Q000254:growth & development / Q000378:metabolism*; D004852:Epoxy Compounds / Q000378:metabolism; D008697:Methane / Q000378:metabolism*; D020581:Methylosinus / Q000378:metabolism; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism; D010710:Phosphates / Q000494:pharmacology; D012988:Soil Microbiology
D004852:Epoxy Compounds; D010710:Phosphates; D010105:Oxygenases; C021555:methane monooxygenase; D008697:Methane; C009068:propylene oxide