title: Doxazosin blockade of alpha 1-adrenergic receptors increases rat serum progesterone levels: a putative role of ovarian angiotensin III in steroidogenesis.
abstract: OBJECTIVE
  To analyze the role of the local renin-angiotensin system (RAS) in the female reproductive system to modulate ovarian steroidogenesis and its relationship with alpha(1)adrenergic receptors.
  
  
  METHODS
  Observational study.
  
  
  METHODS
  University laboratory.
  
  
  METHODS
  Adult female Wistar rats treated with doxazosin (10 mg/kg) or vehicle for 15 days.
  
  
  METHODS
  Samples from the whole right ovary were dissected after perfusion with saline. The soluble and membrane-bound fractions were obtained from these samples. Also, blood samples were used to obtain the serum.
  
  
  METHODS
  Fluorometric measurement of soluble and membrane-bound RAS-regulating proteolytic regulatory enzyme activities by using arylamide derivatives as substrates. Time-resolved fluoroimmunoassay of serum E(2) and P.
  
  
  RESULTS
  alpha(1)Adrenergic receptor blockade increases ovarian soluble and membrane-bound aminopeptidase A and decreases membrane-bound aminopeptidase N and aminopeptidase B. Furthermore, serum P levels increased, whereas serum E(2) did not change.
  
  
  CONCLUSIONS
  Ovarian P production, at least in the rat, is regulated by noradrenaline through a mechanism of action in which the RAS is involved, with a main role for angiotensin III.
authors: Experimental and Clinical Physiopathology Research Group, Department of Health Sciences, Faculty of Experimental and Health Sciences, University of Jaén, Jaén, Spain.
authors: Susana
authors: S
authors: de la Chica-Rodríguez
authors: Pedro
authors: P
authors: Cortés-Denia
authors: María Jesús
authors: MJ
authors: Ramírez-Expósito
authors: José Manuel Arias
authors: JM
authors: de Saavedra
authors: Rafael
authors: R
authors: Sánchez-Agesta
authors: María del Cármen
authors: Mdel C
authors: Pérez
authors: José Manuel
authors: JM
authors: Martínez-Martos
mesh_terms: D058668:Adrenergic alpha-1 Receptor Antagonists; D000805:Angiotensin III / Q000502:physiology*; D000818:Animals; D017292:Doxazosin / Q000494:pharmacology*; D005260:Female; D010053:Ovary / Q000187:drug effects / Q000378:metabolism*; D011374:Progesterone / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar; D018340:Receptors, Adrenergic, alpha-1 / Q000378:metabolism; D013256:Steroids / Q000096:biosynthesis*
chemical_list: D058668:Adrenergic alpha-1 Receptor Antagonists; D018340:Receptors, Adrenergic, alpha-1; D013256:Steroids; D000805:Angiotensin III; D011374:Progesterone; D017292:Doxazosin