Micro-patterned nanofibrous biomaterials.
Nanofibrous materials made from bioabsorbable and biocompatible polymers have promising applications as tissue-engineered scaffolds. Genetic analysis of human umbilical vein endothelial cells (HUVEC) that attached to Poly(glycolide) (PGA) nanofibrous materials prepared via electrospinning methods demonstrated high expression of Integrin v and VEGF receptor genes, which are known angiogenesis markers. In order to improve the function of the PGA nanofibrous materials for tissue engineering applications, we used a micro-patterned template instead of a flat collector in the electrospinning process. "Micro-patterned nanofibrous material" demonstrated uniformly sized dents with diameters of 200 micrometers and depths of 36 micrometers. The dents were regularly spaced, with a 250 micrometer space between two dents. These sizes are similar to that of the template. We will discuss further applications of this designable micro-patterned nanofibrous biomaterial.
Igarashi
Satoshi
S
National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan.
Tanaka
Junzo
J
Kobayashi
Hisatoshi
H
D001483:Base Sequence; D001672:Biocompatible Materials; D002448:Cell Adhesion; D020099:Coated Materials, Biocompatible; D017931:DNA Primers / Q000235:genetics; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D016023:Integrins / Q000235:genetics; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures; D036103:Nanotechnology; D011100:Polyglycolic Acid; D040262:Receptors, Vascular Endothelial Growth Factor / Q000235:genetics; D013499:Surface Properties; D023822:Tissue Engineering
D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D017931:DNA Primers; D016023:Integrins; D011100:Polyglycolic Acid; D040262:Receptors, Vascular Endothelial Growth Factor