Effect of Lactarius piperatus fruiting body maturity stage on antioxidant activity measured by several biochemical assays.
The effects of fruiting body maturity on antioxidant activity and antioxidants production of the wild mushroom, Lactarius piperatus, were evaluated. Several biochemical assays were used to screen the antioxidant properties: reducing power, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity, inhibition of erythrocytes hemolysis mediated by peroxyl radicals and inhibition of lipid peroxidation using the beta-carotene linoleate model system. The amounts of phenols, flavonoids, ascorbic acid, beta-carotene and lycopene present in the immature, mature and degraded fruiting bodies were also determined. The highest antioxidant contents and the lowest EC(50) values for antioxidant activity were obtained in the mature stage with immature spores.
Barros
Lillian
L
CIMO-Escola Superior Agrária, Instituto Politécnico de Bragança, Campus de Sta. Apolónia, Apartado 1172, 5301-855 Bragança, Portugal.
Baptista
Paula
P
Ferreira
Isabel C F R
IC
D000975:Antioxidants / Q000032:analysis* / Q000378:metabolism / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000032:analysis / Q000494:pharmacology; D001487:Basidiomycota / Q000737:chemistry*; D001713:Biphenyl Compounds; D002338:Carotenoids / Q000032:analysis / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology; D005504:Food Analysis / Q000379:methods*; D016166:Free Radical Scavengers / Q000378:metabolism; D006834:Hydrazines; D015227:Lipid Peroxidation / Q000187:drug effects; D000077276:Lycopene; D010084:Oxidation-Reduction; D010636:Phenols / Q000032:analysis / Q000494:pharmacology; D010851:Picrates; D013172:Spores, Fungal / Q000737:chemistry; D019207:beta Carotene / Q000032:analysis / Q000494:pharmacology
D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D016166:Free Radical Scavengers; D006834:Hydrazines; D010636:Phenols; D010851:Picrates; D019207:beta Carotene; D002338:Carotenoids; C004931:1,1-diphenyl-2-picrylhydrazyl; D001205:Ascorbic Acid; D000077276:Lycopene