Phenotype and gene expression pattern of osteoblast-like cells cultured on polystyrene and hydroxyapatite with pre-adsorbed type-I collagen.
Hydroxyapatite and type-I collagen are major components of bone matrix. We compared phenotype and gene expression pattern of osteoblast-like cells cultured on HAp and HAp with pre-adsorbed type-I collagen from neutral solutions (HAp/NCs) with those of tissue culture grade polystyrene (TCPS) and TCPS with the collagen (TCPS/NCs). In initial cell attachment, the cells tensely spread on TCPS, while loosely spread on TCPS/NCs, HAp, and HAp/NCs. The levels of expressed integrin alpha2 and alpha5 subunits were not significantly different among any surfaces. Although the cells on HAp/NCs directly attached with the same collagen molecules as TCPS/NCs, mineralization was observed in only TCPS/NCs. The basal substrates (TCPS and HAp) greatly influenced osteoblast maturation even in the surfaces with pre-adsorbed collagen, since mineralization was induced by TCPS, but not by HAp. Gene expression pattern analyzed with DNA microarray also supported greater influence of basal substrates than pre-adsorbed collagen. In addition, comprehensive gene expression analyses revealed that HAp and HAp/NCs specifically up-regulated Ly6a and Tmem37 genes, while down-regulated Ifitm5 gene.
Hanagata
Nobutaka
N
Biomaterials Center, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan. HANAGATA.Nobutaka@nims.go.jp
Takemura
Taro
T
Monkawa
Akira
A
Ikoma
Toshiyuki
T
Tanaka
Junzo
J
D000327:Adsorption; D000818:Animals; D002113:Calcification, Physiologic; D002448:Cell Adhesion; D048430:Cell Shape; D002478:Cells, Cultured; D016000:Cluster Analysis; D024042:Collagen Type I / Q000378:metabolism*; D017886:Durapatite / Q000378:metabolism*; D005786:Gene Expression Regulation; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism*; D010012:Osteogenesis; D010641:Phenotype; D011137:Polystyrenes / Q000378:metabolism*; D012996:Solutions
D024042:Collagen Type I; D011137:Polystyrenes; D012996:Solutions; D017886:Durapatite