title: Marked difference between self-aggregations of first and fourth repeat peptides on tau microtubule-binding domain in acidic solution.
abstract: The heparin-induced self-aggregation behaviours of four repeat peptides (R1-R4) in an acidic solution (pH = 4.5) were investigated by fluorescence and circular dichroism (CD) measurements and compared with those in a neutral solution (pH = 7.5). In contrast with the self-aggregation-resistive behaviours of the R1 and R4 repeat peptides in the neutral solution, the R4 peptide formed a filament similarly to the R2 and R3 peptides in the acidic solution, whereas the R1 peptide still showed resistive behaviour for filament formation. This is the first report on the markedly different self-aggregation behaviours of the first and fourth repeat peptides on tau microtubule-binding domain.
authors: Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
authors: Fumie
authors: F
authors: Mizushima
authors: Katsuhiko
authors: K
authors: Minoura
authors: Koji
authors: K
authors: Tomoo
authors: Miho
authors: M
authors: Sumida
authors: Taizo
authors: T
authors: Taniguchi
authors: Toshimasa
authors: T
authors: Ishida
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002942:Circular Dichroism; D006493:Heparin / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008854:Microscopy, Electron; D008870:Microtubules / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D020449:Repetitive Sequences, Amino Acid; D012996:Solutions / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013997:Time Factors; D016875:tau Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D010455:Peptides; D012996:Solutions; D016875:tau Proteins; D006493:Heparin