title: Intraspecific variation within Phlebotomus sergenti Parrot (1917) (Diptera: Psychodidae) based on mtDNA sequences in Islamic Republic of Iran.
abstract: An intraspecific study on the morphological and molecular characteristics of Phlebotomus sergenti s.l., the main vector of Leishmania tropica, was performed on 28 Iranian populations from 11 provinces and a few samples from Greece, Morocco, Lebanon, Turkey, Pakistan, and Syria. Three morphotypes were identified as A, B and C, with some intermediate forms in the samples under investigation. Based on the number of setae and the width of basal lobe of coxite, differences between A and B morphotypes were highly significant. Excluding one unusual haplotype, sequence analysis of approximately 439 bp of mtDNA (a fragment of cytochrome B gene, tRNA for serine gene, and a fragment of NADH1 gene) revealed a 6-7% genetic distance within the Iranian populations and among the specimens of other countries. Neighbor-Joining (NJ) analysis confirmed the existence of three main groups within our samples. Although there was no consistency between morphotypes and genotypes, but an interrelationship was found between morphometry and morphotypes. Morphotype A, which was considered as P. sergenti sergenti, was the most prevalent in collection sites. Morphotype B, which was identified as Phlebotomus sergenti similis, is the first record of this subspecies in Iran, and was found to be sympatric with other morphotypes. Morphotype C had an elongated style in comparison with P. sergenti sergenti. Molecular database showed three main genetic structures. This is the first combined morphological and molecular studies on P. sergenti s.l. in Iran.
authors: Department of Medical Entomology and Vector Control, School of Public Health and Institute of Public Health Research, Medical Sciences/University of Tehran, P.O. Box 6446-14155, Tehran, Iran.
authors: Vahideh
authors: V
authors: Moin-Vaziri
authors: Jérôme
authors: J
authors: Depaquit
authors: Mohammad-Reza
authors: MR
authors: Yaghoobi-Ershadi
authors: Mohammad-Ali
authors: MA
authors: Oshaghi
authors: Pupak
authors: P
authors: Derakhshandeh-Peykar
authors: Hubert
authors: H
authors: Ferté
authors: Matthieu
authors: M
authors: Kaltenbach
authors: Maria Dolores
authors: MD
authors: Bargues
authors: Nicole
authors: N
authors: Léger
authors: Aboulhassan
authors: A
authors: Nadim
mesh_terms: D000818:Animals; D004272:DNA, Mitochondrial / Q000032:analysis / Q000235:genetics*; D014644:Genetic Variation; D006801:Humans; D007303:Insect Vectors; D007492:Iran; D007895:Leishmania tropica / Q000502:physiology; D016773:Leishmaniasis, Cutaneous / Q000469:parasitology / Q000635:transmission; D008297:Male; D008969:Molecular Sequence Data; D010691:Phlebotomus / Q000033:anatomy & histology / Q000145:classification* / Q000235:genetics* / Q000469:parasitology; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial