Pilot study: assessment of interlaboratory variability of sequencing-based typing DNA sequence data quality.
This pilot study showed that variability in the sequence quality scores exists within and between laboratories performing human leukocyte antigen typing by sequence-based typing (SBT). It also showed that the base call score (BCS) system in Assign-SBT is a useful tool for comparing sequence quality between laboratories.
Sayer
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DC
Conexio Genomics, Fremantle, Western Australia, Australia. david@conexio.iinet.net.au
Goodridge
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DM
D000483:Alleles; D006680:HLA Antigens / Q000145:classification / Q000235:genetics* / Q000276:immunology; D006650:Histocompatibility Testing; D006801:Humans; D007753:Laboratories / Q000592:standards*; D010865:Pilot Projects; D011786:Quality Control; D017422:Sequence Analysis, DNA; D012984:Software
D006680:HLA Antigens