title: [Relationship between 5,10-methylenetetrahydrofolate reductase genetic polymorphism and arsenic metabolism].
abstract: OBJECTIVE
  To study the relationship between 5,10-methylenetetrahydrofolate reductase(MTHFR) genetic polymorphism and arsenic metabolism and the role of folate in it.
  
  
  METHODS
  Forty-three individuals who were exposed to arsenic more than 0.05 mg/L in well water were chosen and the vein blood and morning urine was collected; The MTHFR C677 polymorphism was analyzed by PCR-RFLP method ; Urinary inorganic and methylated arsenic were speciated by high performance liquid chromatography combined with hydride-generation atomic fluorescence spectrometry; Microbiological assay was used to estimate serum folate.
  
  
  RESULTS
  Subjects with the genotype of CT/TT had the increased percentage of urinary As3+ (r=27.17, P=0.029) and decreased percentage of urinary dimethylarsinic acid (r=26.57, P=0.033). No association was observed among serum folate, genetic polymorphisms of MTHFR C677 and the percentage of urinary arsenic (P>0.05).
  
  
  CONCLUSIONS
  Genetic polymorphism of MTHFR C677 was significantly associated with arsenic methylation and this effect was not associated with the serum folate.
authors: Department of Occupational & Enviromental Health Sciences, Peking University School of Public Health, Beijing 100083,China.
authors: Fu-rong
authors: FR
authors: Deng
authors: Xin-biao
authors: XB
authors: Guo
authors: Li
authors: L
authors: Chen
authors: Zhi-quan
authors: ZQ
authors: Wang
authors: Kai
authors: K
authors: Zhang
mesh_terms: D001151:Arsenic / Q000378:metabolism* / Q000652:urine; D004781:Environmental Exposure / Q000032:analysis; D005492:Folic Acid / Q000097:blood; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008745:Methylation; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D005492:Folic Acid; D042965:Methylenetetrahydrofolate Reductase (NADPH2); D001151:Arsenic