Ozone therapy in induced endotoxemic shock. II. The effect of ozone therapy upon selected histochemical reactions in organs of rats in endotoxemic shock.
Mitochondria and lysosomes were evaluated by assessment of changes in activity of selected enzymes: lactate dehydrogenase (LDH), succinate dehydrogenase (SDH), adenosinetriphosphatase (ATPase), acid phosphatase (AcPase) and beta-glucuronidase (BG) in rats under profound hypoxia induced by endotoxemic shock. The study was conducted on adult male Wistar rats. The animals formed the following four groups of 15 rats each: control animals (C);-rats receiving intraperitonally O(2)/O(3) (CO), rats receiving of Escherichia coli toxin (LPS) (CL); rats receiving LPS plus oxygen-ozone mixture (OL). Histoenzymatic examinations of liver, kidney, lungs, and heart muscle were performed. Lipopolysaccharide suppressed activities of all the enzymes except for LDH, the activity of which as high as a fourfold increase. The results demonstrated potent, stabilizing and regenerative effects of ozone therapy on body enzymatic processes in course of induced endotoxemic shock in rats, which might prove to be of clinical significance.
Madej
Paweł
P
Department and Clinic of Gynaecological Endocrynology, Medical University of Silesia, ul. Medyków 14, 40-752, Katowice, Poland. pmadej@slam.katowice.pl
Plewka
Andrzej
A
Madej
Janusz A
JA
Plewka
Danuta
D
Mroczka
Wojciech
W
Wilk
Krzysztof
K
Dobrosz
Zuzanna
Z
D000135:Acid Phosphatase / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D005966:Glucuronidase / Q000378:metabolism; D000860:Hypoxia / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D007263:Infusions, Parenteral; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D010083:Oxidants, Photochemical / Q000494:pharmacology*; D010100:Oxygen / Q000494:pharmacology; D010126:Ozone / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012772:Shock, Septic / Q000378:metabolism / Q000473:pathology* / Q000628:therapy*; D013385:Succinate Dehydrogenase / Q000378:metabolism
D015415:Biomarkers; D008070:Lipopolysaccharides; D010083:Oxidants, Photochemical; D010126:Ozone; D007770:L-Lactate Dehydrogenase; D013385:Succinate Dehydrogenase; D000135:Acid Phosphatase; D005966:Glucuronidase; D000251:Adenosine Triphosphatases; D010100:Oxygen