Protective immunity of biodegradable nanoparticle-based vaccine against an experimental challenge with Salmonella Enteritidis in mice.
Salmonella spp. infections transmitted by contaminated poultry and eggs represent a major global health burden. Salmonella enterica serovar. Enteritidis is the leading cause of human salmonellosis worldwide. The cell surface antigens of Salmonella Enteritidis play an important role in the host-pathogen interactions and as such represent potential candidates for subunit-vaccine development. An immunogenic subcellular extract obtained from whole Salmonella Enteritidis cells (HE) was encapsulated in nanoparticles made with the polymer Gantrez (HE-NP). Proteomics was used to investigate the complex protein nature of the HE extract. Immunogenicity and protection studies against lethal Salmonella Enteritidis challenge were performed in BALB/c mice. Increased survival was observed in vaccinated mice as compared to a control group; 80% of the mice immunized with the HE-NP formulation survived even when administered 49 days before the lethal challenge. The cytokines released from in vitro-stimulated spleens showed a strong gamma interferon response in all immunized groups at day 10 post-immunization. However, the immunity induced by HE-NP at day 49 post-immunization suggests the involvement of a TH2 subclass in the protective effect. The potential for mucosal vaccination suggests that HE-nanoparticles may represent an important alternative to the conventional attenuated vaccines against Salmonella Enteritidis.
Ochoa
Javier
J
Department of Microbiology, Universidad de Navarra, 31008 Pamplona, Spain.
Irache
Juan Manuel
JM
Tamayo
Ibai
I
Walz
Alexander
A
DelVecchio
Vito G
VG
Gamazo
Carlos
C
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology; D004337:Drug Carriers; D008401:Gas Chromatography-Mass Spectrometry; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry / Q000382:microbiology; D040901:Proteomics; D012481:Salmonella Infections, Animal / Q000382:microbiology / Q000401:mortality / Q000517:prevention & control*; D022562:Salmonella Vaccines / Q000008:administration & dosage* / Q000737:chemistry / Q000276:immunology; D012477:Salmonella enteritidis / Q000276:immunology / Q000472:pathogenicity*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D018418:Th2 Cells / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D004337:Drug Carriers; D007074:Immunoglobulin G; D022562:Salmonella Vaccines; D007371:Interferon-gamma