Analysis of fullerene-based nanomaterial in serum matrix by CE.
With the increasing interest in using nanoparticles as vehicles for drug delivery and image contrast agents, there is a need to develop assays for their detection and quantitation in complex matrices to facilitate monitoring their biodistribution. In this study, we developed a CE approach for the analysis of two nanoparticles: carboxyfullerene (C3) and dendrofullerene (DF1) in both standard solutions and a serum matrix. These highly soluble, charged C(60) derivatives were characterized by CZE using either a bare or dynamically coated fused-silica capillaries. The resolution of both nanoparticles was slightly lower with the coated capillary; however, their migration times were faster. While separation of the DF1 nanoparticles using MEKC resulted in a greater number of observable peaks, the peak profile of C3 was basically unchanged regardless of whether SDS micelles were added to the running buffers or not. The MEKC and/or CZE assays were then used to quantitate the C3 and DF1 nanoparticles in spiked human serum samples. The quantitation of the nanoparticles was linear from 0-500 microg/mL with detection limits ranging from 0.5 to 6 microg/mL.
Chan
King C
KC
Laboratory of Proteomics and Analytical Technologies, SAIC-Frederick Inc, NCI-Frederick, Frederick, MD 21702, USA. chan@ncifcrf.gov
Patri
Anil K
AK
Veenstra
Timothy D
TD
McNeil
Scott E
SE
Issaq
Haleem J
HJ
D002021:Buffers; D002138:Calibration / Q000592:standards; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D037741:Fullerenes / Q000032:analysis* / Q000097:blood*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D049329:Nanostructures / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D044967:Serum / Q000737:chemistry
D002021:Buffers; D037741:Fullerenes; D007202:Indicators and Reagents