title: Antisense oligonucleotide targeting midkine suppresses in vivo angiogenesis.
abstract: OBJECTIVE
  To evaluate the effect of antisense oligonucleotide targeting midkine (MK-AS) on angiogenesis in chick chorioallantoic membrane (CAM) and in situ human hepatocellular carcinoma (HCC).
  
  
  METHODS
  An in situ human hepatocellular carcinoma (HCC) model and CAM assay were used in this experiment. The effect of MK-AS on angiogenesis was evaluated by cell proliferation assay and hematoxylin-eosin (HE) staining.
  
  
  RESULTS
  MK-AS significantly inhibited human umbilical vein endothelial cells (HUVEC) and in situ human HCC growth. At the same time, MK-AS suppressed the angiogenesis both in human hepatocellular carcinoma cell line (HEPG2)-induced CAM and in situ human HCC tissues.
  
  
  CONCLUSIONS
  MK-AS is an effective antiangiogenesis agent in vivo.
authors: Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital, Huzhou 313000, Zhejiang Province, China. dlc@hzhospital.com
authors: Li-Cheng
authors: LC
authors: Dai
authors: Xiang
authors: X
authors: Wang
authors: Xing
authors: X
authors: Yao
authors: Yong-Liang
authors: YL
authors: Lu
authors: Jin-Liang
authors: JL
authors: Ping
authors: Jian-Fang
authors: JF
authors: He
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000473:pathology; D002460:Cell Line; D002642:Chick Embryo; D016207:Cytokines / Q000235:genetics* / Q000627:therapeutic use; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy / Q000473:pathology; D000078792:Midkine; D009389:Neovascularization, Pathologic / Q000188:drug therapy*; D020319:Oligodeoxyribonucleotides, Antisense / Q000627:therapeutic use*; D023041:Xenograft Model Antitumor Assays
chemical_list: D016207:Cytokines; D020319:Oligodeoxyribonucleotides, Antisense; D000078792:Midkine