Kinetics of mobilization and differentiation of lymphohematopoietic cells during experimental murine schistosomiasis in galectin-3 -/- mice.
Galectin-3 (gal-3), a beta-galactoside-binding animal lectin, plays a role in cell-cell and cell-extracellular matrix interactions. Extracellular gal-3 modulates cell migration and adhesion in several physiological and pathological processes. Gal-3 is highly expressed in activated macrophages. Schistosoma mansoni eggs display a large amount of gal-3 ligands on their surface and elicit a well-characterized, macrophage-dependent, granulomatous, inflammatory reaction. Here, we have investigated the acute and chronic phases of S. mansoni infection in wild-type and gal-3(-/-) mice. In the absence of gal-3, chronic-phase granulomas were smaller in diameter, displaying thinner collagen fibers with a loose orientation. Schistosoma-infected gal-3(-/-) mice had remarkable changes in the monocyte/macrophage, eosinophil, and B lymphocyte subpopulations as compared with the infected wild-type mice. We observed a reduction of macrophage number, an increase in eosinophil absolute number, and a decrease in B lymphocyte subpopulation (B220(+/high) cells) in the periphery during the evolution of the disease in gal-3(-/-) mice. B lymphopenia was followed by an increase of plasma cell number in bone marrow, spleen, and mesenteric lymph nodes of the infected gal-3(-/-) mice. The plasma IgG and IgE levels also increased in these mice. Gal-3 plays a role in the organization, collagen distribution, and mobilization of inflammatory cells to chronic-phase granulomas, niches for extramedullary myelopoiesis, besides interfering with monocyte-to-macrophage and B cell-to-plasma cell differentiation.
Oliveira
F L
FL
Departamento de Histologia e Embriologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Frazão
P
P
Chammas
R
R
Hsu
D K
DK
Liu
F T
FT
Borojevic
R
R
Takiya
C M
CM
El-Cheikh
M C
MC
D000208:Acute Disease; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000502:physiology; D002452:Cell Count; D002454:Cell Differentiation; D002908:Chronic Disease; D003433:Crosses, Genetic; D004804:Eosinophils / Q000166:cytology / Q000502:physiology; D005260:Female; D037502:Galectin 3 / Q000235:genetics*; D006099:Granuloma / Q000209:etiology / Q000473:pathology; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007150:Immunohistochemistry; D007700:Kinetics; D008099:Liver / Q000473:pathology; D008198:Lymph Nodes / Q000166:cytology* / Q000502:physiology; D008231:Lymphopenia; D008264:Macrophages / Q000166:cytology / Q000502:physiology; D008297:Male; D008643:Mesentery / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009000:Monocytes / Q000166:cytology / Q000502:physiology; D010950:Plasma Cells / Q000166:cytology; D012550:Schistosoma mansoni / Q000276:immunology / Q000472:pathogenicity; D012552:Schistosomiasis / Q000276:immunology* / Q000378:metabolism
D037502:Galectin 3; D007074:Immunoglobulin G; D007073:Immunoglobulin E