title: [New approaches to molecular diagnosis of prostatic cancer].
abstract: We compared prostatic proteins in patients operated for adenoma or cancer. 630 protein fractions were obtained from each tissue sample after fractionation by two-dimentional electrophoresis according to O'Farrell. Comparison of the samples from adenoma and cancer showed their difference by 7 proteins among which were isoforms of glyceraldehydes-3-phosphate-dehydrogenase, alpha-collagen and several little known proteins. Most of the cancer patients had the protein with molecular mass 19 kDa and isoelectric point 9.0. By the results of mass-spectrometry this protein was identified as androgen-induced secreted protein AGR2. This protein is considered a potential oncomarker. Prospects of some postgenome technologies for detection of new diagnostic markers of prostatic cancer are discussed.
authors: L I
authors: LI
authors: Kovalev
authors: S S
authors: SS
authors: Shishkin
authors: P Z
authors: PZ
authors: Khasigov
authors: N K
authors: NK
authors: Dzeranov
authors: A V
authors: AV
authors: Kazachenko
authors: M A
authors: MA
authors: Kovaleva
authors: I Iu
authors: IIu
authors: Toropygin
authors: L S
authors: LS
authors: Eremina
authors: S V
authors: SV
authors: Grachev
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis* / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008297:Male; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009363:Neoplasm Proteins / Q000032:analysis* / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology / Q000601:surgery
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins