Neurite outgrowths of neurons with neurotrophin-coated carbon nanotubes.
Multiwalled carbon nanotubes (CNTs) coated with neurotrophin were used to regulate the differentiation and survival of neurons. Neurotrophin (nerve growth factor [NGF] or brain-derived neurotrophic factor [BDNF]) was covalently bound to CNTs modified by amino groups using a 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) reagent. The CNTs coated with NGF or BDNF promoted the neurite outgrowths of neurons in the same manner as soluble NGF and soluble BDNF. By enzyme-linked immunosorbent assay (ELISA), we demonstrated that neurotrophin-coated CNTs carry neurotrophin. These results suggest that neurotrophin-coated CNTs have biological activity and stimulate the neurite outgrowths of neurons.
Matsumoto
Kotaro
K
Faculty of Life Sciences, Toyo University, 1-1-1 Izumino, Itakura-machi, Ora-gun, Gunma 374-0193, Japan.
Sato
Chie
C
Naka
Yukie
Y
Kitazawa
Ayako
A
Whitby
Raymond L D
RL
Shimizu
Norio
N
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology; D002642:Chick Embryo; D020099:Coated Materials, Biocompatible; D005727:Ganglia, Spinal / Q000166:cytology; D066298:In Vitro Techniques; D037742:Nanotubes, Carbon; D009414:Nerve Growth Factors / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects* / Q000648:ultrastructure*; D009474:Neurons / Q000187:drug effects* / Q000648:ultrastructure*
D019208:Brain-Derived Neurotrophic Factor; D020099:Coated Materials, Biocompatible; D037742:Nanotubes, Carbon; D009414:Nerve Growth Factors