title: Global analyses of mRNA translational control during early Drosophila embryogenesis.
abstract: BACKGROUND
  In many animals, the first few hours of life proceed with little or no transcription, and developmental regulation at these early stages is dependent on maternal cytoplasm rather than the zygotic nucleus. Translational control is critical for early Drosophila embryogenesis and is exerted mainly at the gene level. To understand post-transcriptional regulation during Drosophila early embryonic development, we used sucrose polysomal gradient analyses and GeneChip analysis to illustrate the translation profile of individual mRNAs.
  
  
  RESULTS
  We determined ribosomal density and ribosomal occupancy of over 10,000 transcripts during the first ten hours after egg laying.
  
  
  CONCLUSIONS
  We report the extent and general nature of gene regulation at the translational level during early Drosophila embryogenesis on a genome-wide basis. The diversity of the translation profiles indicates multiple mechanisms modulating transcript-specific translation. Cluster analyses suggest that the genes involved in some biological processes are co-regulated at the translational level at certain developmental stages.
authors: Howard Hughes Medical Institute, Department of Molecular & Cellular Biology, University of California, Berkeley, Berkeley, CA 94720, USA. xlchin@gmail.com
authors: Xiaoli
authors: X
authors: Qin
authors: Soyeon
authors: S
authors: Ahn
authors: Terence P
authors: TP
authors: Speed
authors: Gerald M
authors: GM
authors: Rubin
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D004330:Drosophila / Q000196:embryology* / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D047108:Embryonic Development / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D038641:RNA 5' Terminal Oligopyrimidine Sequence / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism
chemical_list: D012333:RNA, Messenger