A yeast two-hybrid smart-pool-array system for protein-interaction mapping.
We present here a new two-hybrid smart pool array (SPA) system in which, instead of individual activation domain strains, well-designed activation domain pools are screened in an array format that allows built-in replication and prey-bait deconvolution. Using this method, a Saccharomyces cerevisiae genome SPA increases yeast two-hybrid screening efficiency by an order of magnitude.
Jin
Fulai
F
Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, and the Molecular Biology Institute, University of California, Los Angeles, California 90095, USA.
Avramova
Larisa
L
Huang
Jing
J
Hazbun
Tony
T
D016681:Genome, Fungal / Q000235:genetics; D025941:Protein Interaction Mapping / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D020798:Two-Hybrid System Techniques
D029701:Saccharomyces cerevisiae Proteins