MRI monitoring of focal cerebral ischemia in peroxisome proliferator-activated receptor (PPAR)-deficient mice.
Peroxisome proliferator-activated receptors (PPARs) are a potential target for neuroprotection in focal ischemic stroke. These nuclear receptors have major effects in lipid metabolism, but they are also involved in inflammatory processes. Three PPAR isotypes have been identified: alpha, beta (or delta) and gamma. The development of PPAR transgenic mice offers a promising tool for prospective therapeutic studies. This study used MRI to assess the role of PPARalpha and PPARbeta in the development of stroke. Permanent middle cerebral artery occlusion induced focal ischemia in wild-type, PPARalpha-null mice and PPARbeta-null mice. T(2)-weighted MRI was performed with a 7 T MRI scan on day 0, 1, 3, 7 and 14 to monitor lesion growth in the various genotypes. General Linear Model statistical analysis found a significant difference in lesion volume between wild-type and PPAR-null mice for both alpha and beta isotypes. These data validate high-resolution MRI for monitoring cerebral ischemic lesions, and confirm the neuroprotective role of PPARalpha and PPARbeta in the brain.
Pialat
Jean-Baptiste
JB
Université Lyon 1, Laboratoire CREATIS, INSA de Lyon, CNRS UMR 5515, INSERM U630, Villeurbanne, France.
Cho
Tae-Hee
TH
Beuf
Olivier
O
Joye
Elisabeth
E
Moucharrafie
Samir
S
Moucharaffie
Samir
S
Langlois
Jean-Baptiste
JB
Nemoz
Chantal
C
Janier
Marc
M
Berthezene
Yves
Y
Nighoghossian
Norbert
N
Desvergne
Béatrice
B
Wiart
Marlène
M
D000818:Animals; D001929:Brain Edema / Q000473:pathology; D002545:Brain Ischemia / Q000139:chemically induced / Q000175:diagnosis* / Q000378:metabolism*; D002544:Cerebral Infarction / Q000473:pathology; D004058:Diffusion; D008279:Magnetic Resonance Imaging; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D047493:PPAR alpha / Q000172:deficiency*; D048068:PPAR-beta / Q000172:deficiency*; D013997:Time Factors
D047493:PPAR alpha; D048068:PPAR-beta