Expression and functional role of formyl peptide receptor in human bone marrow-derived mesenchymal stem cells.
We investigated the expression of formyl peptide receptor (FPR) and its functional role in human bone marrow-derived mesenchymal stem cells (MSCs). We analyzed the expression of FPR by using ligand-binding assay with radio-labeled N-formyl-met-leu-phe (fMLF), and found that MSCs express FPR. FMLF stimulated intracellular calcium increase, mitogen-activated protein kinases activation, and Akt activation, which were mediated by G(i) proteins. MSCs were chemotactically migrated to fMLF. FMLF-induced MSC chemotaxis was also completely inhibited by pertussis toxin, LY294002, and PD98059, indicating the role of G(i) proteins, phosphoinositide 3-kinase, and extracellular signal regulated protein kinase. N-terminal fragment of annexin-1, Anx-1(2-26), an endogenous agonist for FPR, also induced chemotactic migration of MSCs. Thus MSCs express functional FPR, suggesting a new (patho)physiological role of FPR and its ligands in regulating MSC trafficking during induction of injured tissue repair.
Kim
Mi-Kyoung
MK
Department of Biochemistry, College of Medicine, Dong-A University, Busan 602-714, Republic of Korea.
Min
Do Sik
do S
Park
Yoon Jeong
YJ
Kim
Jae Ho
JH
Ryu
Sung Ho
SH
Bae
Yoe-Sik
YS
D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D002633:Chemotaxis / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000378:metabolism*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D051058:Oncogene Protein v-akt / Q000378:metabolism; D044042:Receptors, Formyl Peptide / Q000378:metabolism* / Q000502:physiology*
D044042:Receptors, Formyl Peptide; D009240:N-Formylmethionine Leucyl-Phenylalanine; D051058:Oncogene Protein v-akt; D048049:Extracellular Signal-Regulated MAP Kinases; D019204:GTP-Binding Proteins; D002118:Calcium