[An analysis of the DNA fingerprinting of intestinal flora in inflammatory bowel disease].
OBJECTIVE
DNA fingerprinting for inflammatory bowel disease (IBD) patients and healthy subjects was carried out to compare the difference of intestinal flora between the two groups.


METHODS
DNA fingerprinting for IBD patients and healthy persons was set up with enterobacterial repetitive intergenic consensus (ERIC-PCR) technology and the difference of intestinal flora between the two groups compared.


RESULTS
DNA fingerprinting of the IBD patients and healthy subjects was identified and a significant difference was noticed between them. There were lots of bands in the DNA fingerprinting of the healthy subjects but few in that of the IBD patients. Strikingly, same distribution of the principal band of DNA fingerprinting was noticed in IBD patients.


CONCLUSIONS
The variety of intestinal flora in healthy subjects is more apparent than that in IBD patients. An unique principal band might be the sequence of the presence of specific etiopathogenetic bacterium, or it might be the combined sequence of mixed bacterial flora.
Li
Run-mei
RM
Department of Gastroenterology, Beijing Army General Hospital, Beijing 100700, China. lrm80@163.com
Han
Ying
Y
Wang
Ji-heng
JH
Wang
Zhi-hong
ZH
D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D002648:Child; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004755:Enterobacteriaceae / Q000235:genetics*; D005243:Feces / Q000382:microbiology; D005260:Female; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction
D004269:DNA, Bacterial