Experimental regeneration of canine larynx: a trial with tissue engineering techniques.
CONCLUSIONS
Since this tissue engineering technique is cost-effective and is less invasive to patients, it may replace conventional approaches in laryngeal reconstructive surgeries.


OBJECTIVE
Laryngeal cancer is one of the most prevalent cancers in the head and neck region, and frequently requires surgical resection. Although there are many ways to reconstruct the larynx after resection, donor tissue is usually required. Recently, tissue engineering techniques have become widely accepted in clinical medicine and have already been applied to some organs. This animal experiment was designed to elucidate the efficacy of laryngeal regeneration using tissue engineering technique.


METHODS
A bioartificial scaffold was designed from a replica of a canine larynx. A dental cast was used to replicate the intricate inside shape of the larynx. After copying its shape on a polypropylene mesh sheet, this sheet was coated with spongy collagen from porcine skin. A hemilaryngectomy was performed on beagle dogs under general anesthesia. Then the scaffold, preclotted with a mixture of peripheral blood and bone marrow-derived stromal cells, was implanted and fixed. The postoperative status was examined fiberscopically.


RESULTS
On the eighth day after the operation, the surface of the implant was covered with soft tissue. Finally, the implant was completely covered with regenerated mucosa.
Yamashita
Masaru
M
Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Omori
Koichi
K
Kanemaru
Shin-Ichi
S
Magrufov
Akhmar
A
Tamura
Yoshihiro
Y
Umeda
Hiroo
H
Kishimoto
Masanao
M
Nakamura
Tatsuo
T
Ito
Juichi
J
D000818:Animals; D004285:Dogs; D007830:Larynx / Q000502:physiology*; D016343:Monitoring, Intraoperative; D011126:Polypropylenes / Q000008:administration & dosage / Q000627:therapeutic use; D012038:Regeneration / Q000502:physiology*; D013526:Surgical Mesh; D023822:Tissue Engineering / Q000379:methods*
D011126:Polypropylenes