title: [Cultured skin cells interaction with polylactide surface coated by different collagen structures].
abstract: The purpose of this work was an optimization of polylactide film surfaces designed for human keratinocytes cultivation. The polylactide films were coated by collagen 1. The experiments showed that uniform covering of polymer surface by collagen, and formation of different collagen structures depend on the mode of the protein application. The differences in collagen distribution on the polymer surface influened the keratinocytes growth in culture. Analysis of keratinocytes alignment, as well as cytoskeleton organization demonstrated that fibrillar collagen promoted more even keratinocytes distribution in comparison with the distribution on molecular collagen.
authors: Iu A
authors: IuA
authors: Shved
authors: L V
authors: LV
authors: Kukhareva
authors: I M
authors: IM
authors: Zorin
authors: M I
authors: MI
authors: Blinova
authors: A Iu
authors: AIu
authors: Bilibin
authors: G P
authors: GP
authors: Pinaev
mesh_terms: D020341:Absorbable Implants; D001672:Biocompatible Materials / Q000378:metabolism; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000502:physiology; D011091:Polyesters / Q000378:metabolism*; D017768:Skin, Artificial; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D024042:Collagen Type I; D011091:Polyesters; C033616:poly(lactide)