title: Explaining cyclodextrin-mycotoxin interactions using a 'natural' force field.
abstract: Docking techniques and the HINT (Hydropathic Interaction) program were used to explain interactions of aflatoxin B(1) and ochratoxin A with beta- and gamma-cyclodextrins. The work was aimed at designing a chemosensor to identify very low concentrations of these mycotoxins by exploiting the affinity of the cyclodextrin cavity for many small organic molecules. Actually, the inclusion of the fluorescent portion of these toxins into the cavity may lower the quenching effect of the solvent, thus enhancing the luminescence. HINT is a 'natural' force field, based on experimentally determined LogP(octanol/water) values, that is able to consider both enthalpic and entropic contributions to the binding free energy with an unified approach. HINT is normally applied to predict the DeltaG degrees of binding for protein-ligand, protein-protein, and protein-DNA interactions. The leading forces in biomolecular processes are the same as those involved in organic host-guest inclusion phenomena, therefore we applied this methodology for the first time to cyclodextrin complexes. The results allowed us to explain spectroscopic data in absence of available crystallographic or NMR structural data.
authors: Department of Biochemistry and Molecular Biology, University of Parma, I-43100 Parma, Italy.
authors: Alessio
authors: A
authors: Amadasi
authors: Chiara
authors: C
authors: Dall'asta
authors: Gianluigi
authors: G
authors: Ingletto
authors: Roberto
authors: R
authors: Pela
authors: Rosangela
authors: R
authors: Marchelli
authors: Pietro
authors: P
authors: Cozzini
mesh_terms: D016604:Aflatoxin B1 / Q000737:chemistry; D002942:Circular Dichroism; D003505:Cyclodextrins / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D008024:Ligands; D046228:Microarray Analysis; D008958:Models, Molecular; D009183:Mycotoxins / Q000737:chemistry*; D009793:Ochratoxins / Q000737:chemistry; D012984:Software; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship
chemical_list: D003505:Cyclodextrins; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D008024:Ligands; D009183:Mycotoxins; D009793:Ochratoxins; C025589:ochratoxin A; D016604:Aflatoxin B1