Antisense expression of a gene encoding a calcium-binding protein in transgenic tobacco leads to altered morphology and enhanced chlorophyll.
Entamoeba histolytica contains a novel calcium-binding protein like calmodulin,which was discovered earlier,and we have reported the presence of its homologue(s)and a dependent protein kinase in plants.To understand the functions of these in plants,a cDNA encoding a calcium-binding protein isolated from Entamoeba histolytica (EhCaBP)was cloned into vector pBI121 in antisense orientation and transgenic tobacco plants were raised.These plants showed variation in several phenotypic characters,of which two distinct features,more greenness and leaf thickness,were inherited in subsequent generations.The increase in the level of total chlorophyll in different plants ranged from 60% to 70%.There was no major change in chloroplast structure and in the protein level of D1,D2,LHCP and RuBP carboxylase.These morphological changes were not seen in antisense calmodulin transgenic tobacco plants,nor was the calmodulin level altered in EhCaBP antisense plants.
Pandey
Girdhar K
GK
International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110 067, India. giridhar98@gmail.com
Pandey
Amita
A
Reddy
Vanga Siva
VS
Deswal
Renu
R
Bhattacharya
Alok
A
Upadhyaya
Kailash C
KC
Sopory
Sudhir K
SK
D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D002734:Chlorophyll / Q000096:biosynthesis*; D003583:Cytokinins / Q000378:metabolism; D016373:DNA, Antisense / Q000378:metabolism*; D004748:Entamoeba histolytica / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D005822:Genetic Vectors / Q000235:genetics; D046529:Microscopy, Electron, Transmission; D010641:Phenotype; D018515:Plant Leaves / Q000378:metabolism / Q000648:ultrastructure; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D011073:Polyamines / Q000378:metabolism; D014026:Nicotiana / Q000033:anatomy & histology / Q000235:genetics* / Q000378:metabolism
D002135:Calcium-Binding Proteins; D003583:Cytokinins; D016373:DNA, Antisense; D011073:Polyamines; D002734:Chlorophyll