Hyperglycemia and diabetic renal change in a model of polyvinyl alcohol bioartificial pancreas transplantation.
OBJECTIVE
We have developed a bioartificial pancreas transplantation method using polyvinyl alcohol. Using this model, the relationship between hyperglycemia and parameters that represent renal function was investigated.


METHODS
Plasma glucose, 1,5-anhydro-d-glucitol (1,5-AG), and renal factors including plasma urea nitrogen and creatinine levels, urine volume, glucose, and albumin were examined once a week for 9 weeks in diabetic mice with or without transplantation of encapsulated rat islets, and in normal C57BL/6 mice. The mesangial matrix fraction of the glomerulus was measured histologically. The mice were classified into 3 groups according to their mean plasma glucose levels as either severe (n = 17) or mild (n = 23) hyperglycemia or normoglycemia (n = 11). The plasma glucose, renal factors, and mesangial matrix fraction were tested by single and multiple regression analyses.


RESULTS
Almost all the renal factors correlated significantly with mean plasma glucose and 1,5-AG levels. The level and duration of hyperglycemia estimated by the area under the curve above basal correlated most significantly with mesangial matrix fraction.


CONCLUSIONS
Bioartificial pancreas transplantation significantly reduced the deterioration of renal factors. The 1,5-AG was useful to predict urine albumin loss. The level and duration of hyperglycemia determined the degree of renal damage, which were reduced by bioartificial pancreas transplantation.
Sakata
Naoaki
N
Department of Organ Reconstruction and Biomaterials, Institute for Frontier Medical Sciences, Kyoto University, Shogoin-Kawara-cho, Sakyo-ku, Kyoto, Japan.
Sumi
Shoichiro
S
Gu
Yuanjun
Y
Qi
Meirigeng
M
Yamamoto
Chiduru
C
Sunamura
Makoto
M
Egawa
Shinichi
S
Unno
Michiaki
M
Matsuno
Seiki
S
Inoue
Kazutomo
K
D000818:Animals; D021522:Bioartificial Organs; D001786:Blood Glucose / Q000378:metabolism; D003847:Deoxyglucose / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000150:complications / Q000473:pathology / Q000601:surgery* / Q000652:urine; D003928:Diabetic Nephropathies / Q000097:blood / Q000209:etiology* / Q000473:pathology / Q000601:surgery / Q000652:urine; D005782:Gels; D005920:Glomerular Mesangium / Q000473:pathology; D006943:Hyperglycemia / Q000097:blood / Q000150:complications* / Q000209:etiology / Q000473:pathology / Q000601:surgery / Q000652:urine; D007328:Insulin / Q000097:blood; D007515:Islets of Langerhans / Q000378:metabolism; D007677:Kidney Function Tests; D016014:Linear Models; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016035:Pancreas Transplantation / Q000379:methods*; D019397:Pancreas, Artificial; D011142:Polyvinyl Alcohol; D051381:Rats; D017208:Rats, Wistar; D012720:Severity of Illness Index; D013997:Time Factors; D046509:Tissue Culture Techniques; D023822:Tissue Engineering / Q000379:methods; D014183:Transplantation, Heterologous
D001786:Blood Glucose; D005782:Gels; D007328:Insulin; C006584:1,5-anhydroglucitol; D011142:Polyvinyl Alcohol; D003847:Deoxyglucose