Biodegradable fibrous scaffolds composed of gelatin coated poly(epsilon-caprolactone) prepared by coaxial electrospinning.
A facile coaxial electrospinning technique was devised to prepare biodegradable core-shell fibrous scaffolds with poly(epsilon-caprolactone) (PCL) comprising the core structure and gelatin forming the coating of the fibers. The effect of the feed rate of the inner dope on the electrospinning process and fiber morphology was investigated. The results indicated that core-shell fibers with narrow size distribution and smooth surface morphology could be obtained when the feed rate was below 8 mL/h. An increase of the feed rate resulted in analogous increase in the diameters of both the inner PCL fiber core and the entire core-shell fibers. XPS analyses revealed that the surface of the core-shell fibers was tainted with a small amount of PCL. The outer gelatin layer in the core-shell fibers was crosslinked with glutaraldehyde. By optimizing the glutaraldehyde/gelatin feed ratio, crosslinked scaffolds with high porosity were obtained. The mechanic strength of the hydrated, crosslinked core-shell fibrous scaffolds was significantly enhanced because of the presence of hydrophobic PCL in the core region of the fibers. Results of cell culture studies suggested that the crosslinked, core-shell fibrous scaffold were nontoxic and capable of supporting fibroblast adhesion and proliferation.
Zhao
Pengcheng
P
Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
Jiang
Hongliang
H
Pan
Hui
H
Zhu
Kangjie
K
Chen
Weiliam
W
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000378:metabolism*; D003432:Cross-Linking Reagents / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005780:Gelatin / Q000378:metabolism*; D005976:Glutaral / Q000494:pharmacology; D008422:Materials Testing / Q000379:methods*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D011091:Polyesters / Q000378:metabolism*
D020099:Coated Materials, Biocompatible; D003432:Cross-Linking Reagents; D011091:Polyesters; C016240:polycaprolactone; D005780:Gelatin; D005976:Glutaral