Difructose anhydride III promotes absorption of the soluble flavonoid alphaG-rutin in rats.
A highly soluble quercetin glycoside, alphaG-rutin, is a glucose adduct of insoluble rutin. We examined the effects of difructose anhydride III (DFAIII; di-D-fructofuranosyl 1,2':2,3'-dianhydride) on intestinal absorption of alphaG-rutin by rat experiments. alphaG-rutin, rutin, quercetin, and the quercetin conjugate appeared in the portal blood after an intubation of alphaG-rutin (100 micromol), DFAIII effected higher portal concentrations of alphaG-rutin in portal cannulated rats. In ligated jejunal and ileal loops of rats, alphaG-rutin, rutin, quercetin, and the quercetin conjugate appeared in the intestinal mesenteric blood at both 30 and 60 min in both loops; the concentration of alphaG-rutin was 1.5-3 times higher when absorbed in the presence DFAIII. In the isolated mucosae of the jejunum and ileum, mucosal-to-serosal passage of alphaG-rutin increased with the addition of 100 micromol of DFAIII. These results indicate that alphaG-rutin is absorbed as the intact form and that DFAIII stimulates its absorption in the small intestine.
Matsumoto
Megumi
M
Department of Food Science and Human Nutrition, Faculty of Human Life Science, Fuji Women's University, Ishikari, Hokkaido 061-3204, Japan.
Matsukawa
Noriko
N
Chiji
Hideyuki
H
Hara
Hiroshi
H
D000818:Animals; D004187:Disaccharides / Q000494:pharmacology*; D007408:Intestinal Absorption / Q000187:drug effects*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051381:Rats; D012431:Rutin / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D014312:Trisaccharides / Q000097:blood / Q000493:pharmacokinetics*
D004187:Disaccharides; D014312:Trisaccharides; C069037:4(G)-alpha-glucopyranosyl-rutin; D012431:Rutin; C087758:difructose anhydride III