title: Structure and allele-specific expression variation of novel alpha/beta hydrolase fold proteins in gentian plants.
abstract: Previously, we identified two closely related proteins termed W14 and W15 that were enriched in the overwinter buds of the gentian plant Gentiana triflora. Expression of the latter protein W15 has been implicated in its association with cold hardiness, because of its absence in a cold-sensitive mutant. Here, we characterized these two proteins and the genes encoding them. Amino acid sequences of the W14 and W15 proteins showed difference at only three amino acid positions, and both of them showed homologies to alpha/beta hydrolase fold superfamily. Consistently, GST-fused W14 and W15 proteins expressed in bacteria showed hydrolase activity toward 1-naphtyl acetate. Structural analysis of these two genes in seven different gentian strains/cultivars including an anther culture-derived homozygous diploid revealed that W14 and W15 genes are allelic. Three genotypes were found; two strains carried both alleles (W14/W15), one carried the W15 genes in both alleles (W15/W15), and others were homozygous of W14 (W14/W14). Interestingly, expression of the two proteins exhibited allele-specificity. In one W14/W15 strain, expression of the W15 allele was almost repressed. In addition, organ specific expression of the alleles was observed in different cultivars. These observations were discussed in relation to winter hardiness of the gentian plants.
authors: Cryobiosystem Research Center, Faculty of Agriculture, Iwate University, Morioka, Iwate, 020-8550, Japan.
authors: Takashi
authors: T
authors: Hikage
authors: Yasushi
authors: Y
authors: Saitoh
authors: Claire
authors: C
authors: Tanaka-Saito
authors: Hisakazu
authors: H
authors: Hagami
authors: Fumi
authors: F
authors: Satou
authors: Yoshitaka
authors: Y
authors: Shimotai
authors: Yuki
authors: Y
authors: Nakano
authors: Miho
authors: M
authors: Takahashi
authors: Yoshihito
authors: Y
authors: Takahata
authors: Ken-Ichi
authors: K
authors: Tsutsumi
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015971:Gene Expression Regulation, Enzymologic; D030012:Gentiana / Q000235:genetics*; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D015398:Signal Transduction