title: Structure-based functional analysis of the replication protein of plasmid R6K: key amino acids at the pi/DNA interface.
abstract: In previous work, we characterized the bases in an iteron of plasmid R6K that are important for the binding of pi protein monomers and dimers. Here we investigate the following six amino acids of pi, encoded by pir, hypothesized to be important for DNA contact: Ser71, Try74, Gly131, Gly211, Arg225, and Arg254.
authors: Department of Bacteriology, University of Wisconsin, 420 Henry Mall, Madison, WI 53706, USA.
authors: Selvi
authors: S
authors: Kunnimalaiyaan
authors: Sheryl A
authors: SA
authors: Rakowski
authors: Marcin
authors: M
authors: Filutowicz
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019281:Dimerization; D008957:Models, Genetic; D008958:Models, Molecular; D009154:Mutation; D010957:Plasmids / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D012093:Replicon / Q000235:genetics / Q000502:physiology
chemical_list: D000596:Amino Acids; D004268:DNA-Binding Proteins; D004247:DNA