A new spectrofluorimetric method for determination of trace amounts 5-hydroxytryptamine in human urine and serum.
A new spectrofluorimetric method was developed for the determination of trace amount of 5-hydroxytryptamine (5-HT) in human urine and serum samples. In the NaAc-HAc buffer solution of pH=5.80, 5-HT can react with formaldehyde-acetylacetone system to form a new compound which sends yellow green fluorescence at 533 nm and the enhanced fluorescence intensity is in proportion to the concentration of 5-HT. Optimum conditions for the determination of 5-HT were also investigated. The dynamic range and detection limit for the determination of 5-HT are 5.35 x 10(-7) approximately 1.07 x 10(-4) mol/L and 2.08 x 10(-7) mol/L, respectively. The developed method is simple, practical and can be successfully applied to determination of 5-HT in human urine and serum samples. Moreover, the enhancement mechanisms of the fluorescence intensity in the 5-HT - formaldehyde-acetylacetone system have been also discussed.
Peng
Qian
Q
College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China.
Jiang
Chongqiu
C
D002138:Calibration; D005557:Formaldehyde / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D010422:Pentanones / Q000737:chemistry; D015203:Reproducibility of Results; D012701:Serotonin / Q000097:blood* / Q000737:chemistry / Q000652:urine*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D010422:Pentanones; D005557:Formaldehyde; D012701:Serotonin; C008790:acetylacetone