A new method for the authentication of plant samples by analyzing fingerprint chromatograms.
Chemical analysis by high-performance liquid chromatography or capillary electrophoresis of plant pulverized samples, juices or extracts is an excellent method for the authentication of medicinal plant species and their products, particularly when morphological authentication is not possible. In the conventional procedure, chromatograms are integrated and the heights or areas of several peaks are used in a supervised pattern recognition method to confirm the authenticity of the product. We propose a new section approach in analysing chromatograms, where chromatograms are split into sections, which are described by four variables (number of peaks in the section, average retention time of peaks in the section, total area of peaks in the section and average area of peaks in the section), and these variables are then used in statistical analysis. The method is especially useful when the peaks on the chromatogram are not well separated and it is not easy to link individual peaks on one chromatogram with corresponding peaks on other chromatograms. In comparison with the standard procedure, our approach in analyzing chromatographic data of willow-herb (Epilobium and Chamaenerion spp.) extracts was more objective, gave better results and was also easier to perform.
Obradovic
Marko
M
Faculty of Pharmacy, University of Ljubljana, Askerdeva 7, SI-1000 Ljubljana, Slovenia. marko.obradovic@ffa.uni-lj.si
Krajsek
Simona Strgulc
SS
Dermastia
Marina
M
Kreft
Samo
S
D002845:Chromatography / Q000379:methods*; D003627:Data Interpretation, Statistical; D019075:Electrophoresis, Capillary; D031666:Epilobium / Q000737:chemistry; D016014:Linear Models; D010936:Plant Extracts / Q000032:analysis; D010944:Plants / Q000737:chemistry*; D015203:Reproducibility of Results; D012997:Solvents; D013045:Species Specificity
D010936:Plant Extracts; D012997:Solvents