title: Candida albicans does not invade carious human dentine.
abstract: OBJECTIVE
  Candida albicans has been proposed to be a caries pathogen, but the evidence for its specific role is lacking. To be considered significant in caries progression, a marked amount of yeasts should be present in a lesion. The aim of the study was to investigate the presence of C. albicans in dentinal caries lesions.
  
  
  METHODS
  To demonstrate the extension of caries and to identify the bacteria in a lesion, sections of 10 carious human teeth were stained with Gram and Giemsa stains. C. albicans was detected with periodic acid-Schiff (PAS) staining and by immunohistochemistry using a C. albicans-specific antibody 3H8. Thirty sections were used for each staining (in total 120 sections).
  
  
  RESULTS
  Extensive bacterial invasion and intensive staining by PAS occurred in all samples. However, with the C. albicans-specific antibody, only 30 (3.3%) sections stained weakly positive, with a few stained cells on the lesion surface. However, the positive identification of C. albicans, based on the morphology of the cells, was not possible.
  
  
  CONCLUSIONS
  The results do not support the previous suggestion that C. albicans is important in the dentine caries pathology. In addition, because of its unspecific nature, PAS turned out to be an unsuitable method for detecting yeasts in carious tooth samples.
authors: Institute of Dentistry, University of Helsinki, Helsinki, Finland.
authors: M
authors: M
authors: Maijala
authors: R
authors: R
authors: Rautemaa
authors: A
authors: A
authors: Järvensivu
authors: M
authors: M
authors: Richardson
authors: T
authors: T
authors: Salo
authors: L
authors: L
authors: Tjäderhane
mesh_terms: D000946:Antigens, Fungal / Q000032:analysis; D002176:Candida albicans / Q000472:pathogenicity*; D003731:Dental Caries / Q000382:microbiology*; D003804:Dentin / Q000382:microbiology*; D006801:Humans; D007150:Immunohistochemistry; D010503:Periodic Acid-Schiff Reaction
chemical_list: D000946:Antigens, Fungal