Striated muscle and nerve fascicle distribution in the female rat urethral sphincter.
The anatomical basis for urinary continence depends on a thorough understanding of the tissues in the urethra. The objective of this study was to evaluate the morphology and neuroanatomy of urethral striated muscle, called the rhabdosphincter or external urethral sphincter, in normal female rats. Urethras from 12 female rats were dissected from the bladder, fixed, embedded in paraffin or epon, and sectioned every 1 mm. Striated muscle content was taken as the ratio of the striated muscle area to net urethral area. Nerve fascicles containing myelinated axons near the rhabdosphincter were counted and mapped. Both striated muscle content and number of nerve fascicles peak in the proximal third of the urethra, with a secondary peak at the distal end of the urethra. This secondary peak may correspond to an analog of the combined compressor urethrae/urethrovaginal sphincter located in the distal urethra in human. The rhabdosphincter has a variable distribution along the length of the urethra. In the middle and distal thirds of the urethra, the dorsal striated muscle fibers between the urethra and vagina become more sparse. The majority of nerve fascicles are contained in the lateral quadrants of the urethra, similar to the lateral distribution of somatic nerves in humans. In conclusion, this study demonstrates the normal distribution of the striated musculature and neuroanatomy in the urethra, with similarities to the human. It thus supports and extends the usefulness of the rat as an experimental model for studying urinary incontinence.
Kim
Ronald J
RJ
Research Service, Hines VA Hospital, Hines, Illinois, USA.
Kerns
James M
JM
Liu
Shirley
S
Nagel
Ted
T
Zaszczurynski
Paul
P
Lin
Dan Li
DL
Damaser
Margot S
MS
D019412:Anatomy, Cross-Sectional; D000818:Animals; D005260:Female; D018482:Muscle, Skeletal / Q000033:anatomy & histology / Q000294:innervation*; D009413:Nerve Fibers, Myelinated; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling; D014521:Urethra / Q000033:anatomy & histology / Q000294:innervation*