title: Double dissociation between long-term depression and dendritic spine morphology in cerebellar Purkinje cells.
abstract: Experiments in hippocampal area CA1 suggest that long-term potentiation could be associated with spine addition and enlargement, and long-term depression (LTD) with spine shrinkage and loss. Is this a general principle of synaptic plasticity? We used two-photon microscopy to measure dendritic spines in rat cerebellar Purkinje cells. Neither local synaptic induction of LTD nor global chemical induction of LTD changed spine number or size. Conversely, a manipulation that evoked persistent dendritic spine retraction did not alter parallel fiber-evoked excitatory postsynaptic currents.
authors: Department of Neuroscience, Johns Hopkins University School of Medicine, 725 N. Wolfe Street, 916 Hunterian Building, Baltimore, Maryland 21205, USA.
authors: Andrei D
authors: AD
authors: Sdrulla
authors: David J
authors: DJ
authors: Linden
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000378:metabolism; D002531:Cerebellum / Q000166:cytology*; D049229:Dendritic Spines / Q000000981:diagnostic imaging* / Q000502:physiology*; D004558:Electric Stimulation / Q000379:methods; D066298:In Vitro Techniques; D036881:Long-Term Synaptic Depression / Q000502:physiology*; D008297:Male; D018408:Patch-Clamp Techniques / Q000379:methods; D011689:Purkinje Cells / Q000648:ultrastructure*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018709:Statistics, Nonparametric; D013268:Stimulation, Chemical; D013997:Time Factors; D014463:Ultrasonography
chemical_list: D002118:Calcium