title: Origin and prognostic value of circulating KRAS mutations in lung cancer patients.
abstract: Because of the current controversy on the origin and clinical value of circulating KRAS codon 12 mutations in lung cancer, we screened 180 patients using a combined restriction fragment-length polymorphism and polymerase chain reaction (RFLP-PCR) assay. We detected KRAS mutations in 9% plasma samples and 0% matched lymphocytes. Plasma KRAS mutations correlated significantly with poor prognosis. We validated the positive results in a second laboratory by DNA sequencing and found matching codon 12 sequences in blood and tumor in 78% evaluable cases. These results support the notion that circulating KRAS mutations originate from tumors and are prognostically relevant in lung cancer.
authors: University of California Davis Cancer Center, 4501 X Street, Sacramento, CA 95817, USA. oliver_gautschi@hotmail.com
authors: O
authors: O
authors: Gautschi
authors: B
authors: B
authors: Huegli
authors: A
authors: A
authors: Ziegler
authors: M
authors: M
authors: Gugger
authors: J
authors: J
authors: Heighway
authors: D
authors: D
authors: Ratschiller
authors: P C
authors: PC
authors: Mack
authors: P H
authors: PH
authors: Gumerlock
authors: H J
authors: HJ
authors: Kung
authors: R A
authors: RA
authors: Stahel
authors: D R
authors: DR
authors: Gandara
authors: D C
authors: DC
authors: Betticher
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004273:DNA, Neoplasm / Q000097:blood / Q000235:genetics; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000401:mortality; D008297:Male; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011379:Prognosis; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016283:Proto-Oncogene Proteins p21(ras); D016019:Survival Analysis; D018631:ras Proteins / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm; C117307:KRAS protein, human; D011518:Proto-Oncogene Proteins; D016283:Proto-Oncogene Proteins p21(ras); D018631:ras Proteins