Microsatellite instability in long-standing ulcerative colitis.
OBJECTIVE
Ulcerative colitis (UC) is a chronic inflammatory disease of the colon associated with a high risk of developing colorectal cancer. Several genetic alterations have been documented in dysplasia and cancer developing in UC. Concerning microsatellite instability (MSI), many contradictory results have been published. We therefore analysed a large, well-characterized UC material for MSI to elucidate its significance in long-standing UC.


METHODS
From 33 patients, a total of 159 microdissected lesions and 165 mucosa samples obtained adjacent to the tissue blocks were analysed for MSI using the five standard markers recommended by the National Cancer Institute; D2S123, D5S346, D17S250, BAT-25 and BAT-26. In addition, 12 of the patients were investigated by a mini-satellite marker at the D1S7 locus.


RESULTS
High-level MSI (MSI-H) was detected in one villous adenoma with high-grade dysplasia and right-sided location. This represents 3.6% (1/28) of dysplastic mucosa investigated. No other lesions showed MSI in the five standard markers or at the D1S7 locus.


CONCLUSIONS
This study suggests that MSI is rare in UC-related neoplasia as well as non-neoplastic lesions, and does not contribute to the development of dysplasia.
Løvig
Tone
T
Institute of Forensic Medicine, Department of Pathology, Akershus University Hospital HF, Lørenskog, Norway. tone.lovig@labmed.uio.no
Andersen
Solveig Norheim
SN
Clausen
Ole Petter
OP
Rognum
Torleiv Ole
TO
D000328:Adult; D000368:Aged; D000483:Alleles; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D003093:Colitis, Ulcerative / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D015179:Colorectal Neoplasms / Q000235:genetics / Q000473:pathology; D004273:DNA, Neoplasm / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005500:Follow-Up Studies; D005819:Genetic Markers; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D008297:Male; D053842:Microsatellite Instability; D018895:Microsatellite Repeats; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011230:Precancerous Conditions / Q000235:genetics / Q000473:pathology; D016737:Proctocolectomy, Restorative; D011379:Prognosis; D013997:Time Factors
C516892:BAT26 microsatellite DNA; D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D005819:Genetic Markers