Berberine inhibits WEHI-3 leukemia cells in vivo.
Berberine, an isoquinoline alkaloid, has a wide range of pharmacological effects including anticancer activities, yet the exact effects on leukemia in vivo are unknown. Our previous studies have demonstrated that berberine induced cytotoxicity against murine leukemia WEHI-3 cells in vitro in a dose-dependent manner. In order to understand the berberine action against leukemia, the effect of berberine on WEHI-3 leukemia cells in vivo was studied. The results showed that Mac-3 and CD11b markers were reduced, indicating differentiation inhibition of the macrophages and granulocytes precursors. There was no affect on the CD14 marker but the CD19 marker that was indicating the promotion of the differentiation of the B-cells precursors. The weights of spleen samples from mice treated with berberine were found to be lower when compared to these from untreated animals.
Yu
Fu-Shun
FS
School of Dentistry, China Medical University, Taichung, Taiwan; 4Department of Life Science, Tunghai University, Taichung, Taiwan.
Yang
Jai-Sing
JS
Lin
Hui-Ju
HJ
Yu
Chun-Shu
CS
Tan
Tzu-Wei
TW
Lin
Yuh-Tzy
YT
Lin
Chin-Chung
CC
Lu
Hsu-Feng
HF
Chung
Jing-Gung
JG
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001599:Berberine / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects*; D045744:Cell Line, Tumor; D007942:Leukemia, Experimental / Q000188:drug therapy* / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000187:drug effects / Q000473:pathology
D000970:Antineoplastic Agents; D001599:Berberine