Glyoxal-induced premature senescence in human fibroblasts.
Mild stress-induced hormesis is an effective strategy to intervene in the aging process. Repeated exposure of human skin fibroblasts to 41 degrees C heat shock for 1 h twice a week is an example of mild stress that has many hormetic effects, including improved resistance to other stressors. We are now developing an experimental model system of sugar-induced premature senescence, which can be useful to test the hormetic and antiaging effects of other stresses. Our present studies show that early-passage human skin fibroblasts treated with 1 mM glyoxal for 72 h undergo premature senescence in terms of enlarged cell size, inhibition of cell division, slowing down of cell growth, a decrease in the number of DNA synthesizing cells, and increased resistance to apoptosis.
Sejersen
Henrik
H
Department of Molecular Biology, University of Aarhus, Gustav Wieds Vej 10C, DK8000 Aarhus-C, Denmark.
Rattan
Suresh I S
SI
D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects*; D004247:DNA / Q000378:metabolism; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000378:metabolism*; D006037:Glyoxal / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D018384:Oxidative Stress; D010641:Phenotype; D012867:Skin / Q000166:cytology / Q000378:metabolism
D000975:Antioxidants; D006037:Glyoxal; D004247:DNA; D001973:Bromodeoxyuridine