Transient loss of high-level mupirocin resistance in Staphylococcus aureus due to MupA polymorphism.
Spontaneous loss of MupA-mediated high-level mupirocin resistance was observed in Staphylococcus aureus, although the isolate gave a PCR-positive test result for mupA. Sequencing of the mupA gene identified a single base-pair deletion that resulted in a frameshift mutation and loss of functional protein. Reversion to the wild-type allele and restoration of high-level resistance occurred with high frequency (>10(-6)), indicating the transient nature of MupA polymorphism.
Driscoll
Daniel G
DG
Replidyne, Inc., Dept. Microbiology, 1450 Infinite Dr., Louisville, CO 80027, USA. uochsner@replidyne.com
Young
Casey L
CL
Ochsner
Urs A
UA
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D016368:Frameshift Mutation; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D008969:Molecular Sequence Data; D016712:Mupirocin / Q000494:pharmacology*; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D009687:Nuclear Proteins; C480380:mupA protein, Staphylococcus aureus; D016712:Mupirocin