[Differences between the expression of matrix metalloproteinase-2, 9 in preeclampsia and normal placental tissues].
OBJECTIVE
To study the possible relationships between expression of matrix metalloproteinase (MMP) 2, 9 and the pathogenesis of preeclampsia in which trophoblast invasion is impaired.


METHODS
MMP-2, 9 expression were detected by immunohistochemistry streptavidin-biotin complex (SABC) method in 20 normal term placentae and 20 preeclampsia placentae, respectively. In addition, mRNAs for MMP-2, 9 were analyzed by real time PCR in both groups.


RESULTS
The intensities of both MMP-2 and MMP-9 immunostaining in preeclampsia placentae were significantly declined compared to those of normal term placentae (P < 0.05). By using 2(-DeltaDeltaCt) as a relatively quantitative assay, mRNA expression of MMP-2 was significantly higher in 10 normal placentae than those in 13 preeclampsia placentae (7.6 +/- 2.8 vs 5.6 +/- 1.5, P < 0.05). Expression of MMP-9 mRNA was also significantly higher in normal placentae than those in preeclampsia placentae (2.2 +/- 2.6 vs -0.9 +/- 2.0, P < 0.05). Whereas, mRNA expression of MMP-2 was higher compared to MMP-9 in normal term placentae (P < 0.05).


CONCLUSIONS
The decreased expression of MMP-2 and MMP-9 in preeclampsia placentae may lead to impaired invasion of trophoblast cells, causing abnormal placentation and occurrence of preeclampsia.
Li
Jin-Ke
JK
Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Xiong
Qing
Q
Zhou
Shu
S
Yang
Pei-Feng
PF
D000328:Adult; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis* / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis* / Q000235:genetics; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000209:etiology / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014327:Trophoblasts / Q000378:metabolism
D012333:RNA, Messenger; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9