title: On the functions of the h subunit of eukaryotic initiation factor 3 in late stages of translation initiation.
abstract: BACKGROUND
  The eukaryotic translation initiation factor 3 (eIF3) has multiple roles during the initiation of translation of cytoplasmic mRNAs. How individual subunits of eIF3 contribute to the translation of specific mRNAs remains poorly understood, however. This is true in particular for those subunits that are not conserved in budding yeast, such as eIF3h.
  
  
  RESULTS
  Working with stable reporter transgenes in Arabidopsis thaliana mutants, it was demonstrated that the h subunit of eIF3 contributes to the efficient translation initiation of mRNAs harboring upstream open reading frames (uORFs) in their 5' leader sequence. uORFs, which can function as devices for translational regulation, are present in over 30% of Arabidopsis mRNAs, and are enriched among mRNAs for transcriptional regulators and protein modifying enzymes. Microarray comparisons of polysome loading in wild-type and eif3h mutant seedlings revealed that eIF3h generally helps to maintain efficient polysome loading of mRNAs harboring multiple uORFs. In addition, however, eIF3h also boosted the polysome loading of mRNAs with long leaders or coding sequences. Moreover, the relative polysome loading of certain functional groups of mRNAs, including ribosomal proteins, was actually increased in the eif3h mutant, suggesting that regulons of translational control can be revealed by mutations in generic translation initiation factors.
  
  
  CONCLUSIONS
  The intact eIF3h protein contributes to efficient translation initiation on 5' leader sequences harboring multiple uORFs, although mRNA features independent of uORFs are also implicated.
authors: Department of Biochemistry, Cellular and Molecular Biology, The University of Tennessee, Knoxville, TN 37996-0840, USA. bkim2@utk.edu
authors: Byung-Hoon
authors: BH
authors: Kim
authors: Xue
authors: X
authors: Cai
authors: Justin N
authors: JN
authors: Vaughn
authors: Albrecht G
authors: AG
authors: von Arnim
mesh_terms: D020121:5' Untranslated Regions / Q000737:chemistry; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000096:biosynthesis* / Q000235:genetics; D039621:Eukaryotic Initiation Factor-3 / Q000502:physiology*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D016366:Open Reading Frames; D010442:Peptide Chain Initiation, Translational; D011132:Polyribosomes / Q000502:physiology; D021122:Protein Subunits / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D017423:Sequence Analysis, RNA; D019076:Transgenes
chemical_list: D020121:5' Untranslated Regions; D029681:Arabidopsis Proteins; D039621:Eukaryotic Initiation Factor-3; D021122:Protein Subunits; D012333:RNA, Messenger