Simultaneous determination of citrus limonoid aglycones and glucosides by high performance liquid chromatography.
High performance liquid chromatography (HPLC) method has been developed for simultaneous quantification of limonoid aglycones and glucosides on a reversed phase C18 column using a binary solvent system, coupled with diode array detector. Seven limonoids such as limonin, nomilin, isolimonic acid, ichangin, isoobacunoic acid, limonin 17-beta-D glucopyranoside and deacetyl nomilinic acid 17-beta-D glucopyranoside were separated and detected at 210 nm. Furthermore, limonoids were separated, identified and quantified in four varieties of citrus fruits and seeds using developed method. Limonin and limonin glucoside were found to be the predominant limonoid aglycone and glucoside, respectively, in all tested samples. The sensitivity of the method was found to be 0.25-0.50 microg for tested limonoids.
Vikram
Amit
A
Vegetable and Fruit Improvement Center, Department of Horticultural Sciences, 1500 Research Parkway Ste A120, Texas A & M University, College Station, TX 77845, United States.
Jayaprakasha
G K
GK
Patil
Bhimanagouda S
BS
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002957:Citrus / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis*; D036701:Limonins / Q000032:analysis*; D012639:Seeds / Q000737:chemistry
D005960:Glucosides; D036701:Limonins