NARCOBIT: a newly developed inhalational anesthesia system for mice.
NARCOBIT is the first anesthetic system for mice and rats to incorporate a ventilator. Therefore, it is expected to improve the reliability of mice and rat experiments by accurately controlling and maintaining the depth of anesthesia. In this study, we used NARCOBIT for inducing inhalational anesthesia in mice and evaluated the changes in their hemodynamic parameters. ICR mice were anesthetized with 5% isoflurane and room air, followed by endotracheal intubation. Subsequently, they were mechanically ventilated, and anesthesia was maintained by 2% isoflurane for a 60-min period (maintenance state) using NARCOBIT. In study 1, the heart rate (HR) and mean arterial blood pressure (MAP) were measured. The skin blood flow (SBF) from the hind legs was continuously measured during the maintenance state. Subsequently, the concentration-dependent effects of isoflurane on MAP were examined. In study 2, blood samples were obtained from the abdominal aorta for blood gas analysis. The HR and MAP decreased after anesthesia but were stable during the maintenance state. Decreased MAP and concentration-dependent effects of isoflurane were observed. The SBF increased slightly during the maintenance state but this increase was insignificant. The blood gas analysis showed neither hypoxia nor hypercapnia. Since the use of NARCOBIT enables the anesthetic concentration of isoflurane to be easily changed, a suitable anesthesia depth can be obtained for experimental purposes. Therefore, we conclude that NARCOBIT can be used for providing inhalational anesthesia to mice.
Matsuda
Yoshikazu
Y
Department of Integrative Physiology & Bio-System Control, Shinshu University School of Medicine, Matsumoto, Nagano, Japan.
Ohsaka
Kazumasa
K
Yamamoto
Hidekazu
H
Jiyouraku
Kouzou
K
Natsume
Katsuhiko
K
Hirabayashi
Shirokazu
S
Kounoike
Masayoshi
M
Inoue
Masaaki
M
D000769:Anesthesia, Inhalation / Q000295:instrumentation* / Q000379:methods; D018685:Anesthetics, Inhalation; D000818:Animals; D000831:Animals, Newborn; D001784:Blood Gas Analysis; D001794:Blood Pressure / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006339:Heart Rate / Q000187:drug effects; D006614:Hindlimb / Q000098:blood supply; D007530:Isoflurane; D051379:Mice; D008813:Mice, Inbred ICR; D012039:Regional Blood Flow / Q000187:drug effects; D012121:Respiration, Artificial; D012867:Skin / Q000098:blood supply
D018685:Anesthetics, Inhalation; D007530:Isoflurane