Electron paramagnetic resonance analyses of biotransformation reactions with cytochrome P-450 immobilized on mesoporous molecular sieves.
Mobil Crystalline Material (MCM-41) can be used for the immobilization of enzymes and the investigation of electron transfer in biological systems. Electron transfer between MCM-41 with aluminum (Al-MCM-41) and cytochrome P-450 (CYP2B4) was observed using electron paramagnetic resonance (EPR). When CYP2B4 was immobilized by adsorption, it catalyzed the conversion of aniline to p-aminophenol. The electron transfer was evidenced when the signal with a g value (also called g-factor or spectroscopic manifestation of the magnetic moment) of 1.98 increased at the same time that the signal with a g value 2.24 decreased due to the addition of NADPH to CYP2B4 immobilized on Al-MCM-41, indicating that FeIII was reduced to FeII. Therefore, it is possible that Al-MCM-41 participates in the electron transfer process in biological systems.
Rosales-Hernández
Martha
M
Sección de Estudios de Posgrado e Investigación y Departamento de Bioquímica de la, Escuela Superior de Medicina del Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón s/n, Mexico, D.F., Mexico.
Kispert
Lowell
L
Torres-Ramírez
Eduardo
E
Ramírez-Rosales
Daniel
D
Zamorano-Ulloa
Rafael
R
Trujillo-Ferrara
Jose
J
D000535:Aluminum / Q000737:chemistry; D000627:Aminophenols / Q000737:chemistry; D000814:Aniline Compounds / Q000737:chemistry; D001711:Biotransformation; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry* / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D004579:Electron Transport; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005290:Ferric Compounds / Q000737:chemistry / Q000493:pharmacokinetics; D009249:NADP / Q000737:chemistry; D010084:Oxidation-Reduction; D014961:X-Ray Diffraction
D000627:Aminophenols; D000814:Aniline Compounds; D004800:Enzymes, Immobilized; D005290:Ferric Compounds; D009249:NADP; D003577:Cytochrome P-450 Enzyme System; D000535:Aluminum; C026729:4-aminophenol; C023650:aniline