Reinforcement of antitumor effect of Cordyceps sinensis by 2'-deoxycoformycin, an adenosine deaminase inhibitor.
In this study, an attempt was made to elucidate the combined effect of 2'-deoxycoformycin (DCF), an adenosine deaminase inhibitor, with a water extract of Cordyceps sinensis (WECS), on the growth curves of mouse melanoma and lung carcinoma cells. Sub-confluent cells were harvested with an EDTA trypsin solution, and resuspended to appropriate concentrations in DMEM containing 10% fetal bovine serum. Using 1x10(5) cells/2 ml in each well of a 12-well culture plate, cells were incubated for 24, 48 and 72 h in the presence of WECS alone, or WECS plus DCF in a CO2 incubator at 37 degrees C. Duplicate samples of viable cells were enumerated with a Coulter counter. The antitumor effect of WECS on the growth curves of tumor cell lines increased over 3-fold in combination with DCF. These results suggest that DCF has a remarkable reinforcement effect on the antitumor activity of WECS. DCF is a potent adjuvant for WECS.
Yoshikawa
Noriko
N
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Hyogo 663-8179, Japan.
Nakamura
Kazuki
K
Yamaguchi
Yu
Y
Kagota
Satomi
S
Shinozuka
Kazumasa
K
Kunitomo
Masaru
M
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects*; D045744:Cell Line, Tumor; D045424:Complex Mixtures / Q000627:therapeutic use*; D032661:Cordyceps; D003839:Deoxyadenosines / Q000494:pharmacology*; D007700:Kinetics; D008516:Medicine, Chinese Traditional; D008545:Melanoma; D051379:Mice; D015649:Pentostatin / Q000494:pharmacology*
D000903:Antibiotics, Antineoplastic; D000970:Antineoplastic Agents; D045424:Complex Mixtures; D003839:Deoxyadenosines; D015649:Pentostatin; C058120:cordycepin