Effect of dietary supplementation of probiotic and vitamin C on the immune response of Indian major carp, Labeo rohita (Ham.).
The immunostimulatory effect of probiotics and vitamin C has been established in many systems including fish. An investigation was carried out to study the effect of dietary supplementation of a probiotic bacterium "Bacillus subtilis", vitamin C in the form of ascorbyl polyphosphate and their combination on the immune response of Indian major carp, rohu, (Labeo rohita Ham.) fingerlings fed for a period of 60 days. The total serum protein and globulin content was significantly higher (p<0.05) in probiotic (B. subtilis @ 10(8) CFU/g of the feed) fed group while the respiratory burst activity of blood neutrophils was significantly high in vitamin C (ascorbyl polyphosphate @100 mg per kg diet) fed group. The antibody level was significantly high in Bacillus subtilis treated group followed by the probiotic (B. subtilis @ 10(8) CFU/g of the feed) and ascorbyl polyphosphate (ascorbyl polyphosphate @100 mg per kg diet) combined group. The least percentage of mortality was recorded in B. subtilis treated group (25%) followed by 35 and 40% in ascorbyl polyphosphate treated and B. subtilis and ascorbyl polyphosphate combined groups, respectively.
Nayak
S K
SK
Central Institute of Freshwater Aquaculture, Fish Health Management Division, Kausalyaganga 751002, Bhubaneswar, Orissa, India.
Swain
P
P
Mukherjee
S C
SC
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology; D001798:Blood Proteins / Q000032:analysis; D002347:Carps / Q000276:immunology*; D019587:Dietary Supplements; D020609:Edwardsiella tarda / Q000737:chemistry / Q000276:immunology; D004756:Enterobacteriaceae Infections / Q000276:immunology / Q000401:mortality / Q000662:veterinary*; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology / Q000401:mortality; D009504:Neutrophils / Q000187:drug effects; D019936:Probiotics / Q000008:administration & dosage; D016897:Respiratory Burst / Q000187:drug effects; D013997:Time Factors
D000907:Antibodies, Bacterial; D000975:Antioxidants; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D001798:Blood Proteins; D001205:Ascorbic Acid