An in vitro model for assessment of the biological activity of hepatocyte growth factor.
Hepatocyte growth factor (HGF) is a multifunctional growth factor with potent wound-healing properties that functions in the healing of chronic injuries. However, there may be a loss of HGF activity in certain chronic cases; this might be indicated by the presence of high amounts of HGF in body fluids and by the elevated expression of the HGF receptor in tissue biopsies. In such cases, a reliable means of assessing the activity of endogenous HGF would be valuable in allowing clinicians to decide if treatment with HGF would be useful. In this study, we developed an in vitro wound assay that used a mouse skin epithelial cell line to evaluate the biological activity of HGF. We showed that HGF accelerated the motility of the epithelial cells in a dose-dependent fashion with high sensitivity and specificity. This in vitro assay might be used to determine the activity of both endogenous and recombinant HGF.
Nayeri
Fariba
F
Division of Infectious Diseases, Faculty of Health Science, Linköping University, Linköping, SE-581 85, Sweden. fariba.nayeri@lio.se
Holmgren-Pettersson
Kajsa
K
Perskvist
Nasrin
N
Forsberg
Pia
P
Peterson
Curt
C
Sundqvist
Tommy
T
D000368:Aged; D000818:Animals; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects; D005260:Female; D017228:Hepatocyte Growth Factor / Q000494:pharmacology / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008875:Middle Aged; D008954:Models, Biological; D011994:Recombinant Proteins / Q000494:pharmacology; D012867:Skin / Q000166:cytology / Q000187:drug effects; D012883:Skin Ulcer / Q000503:physiopathology; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology*
D011994:Recombinant Proteins; D017228:Hepatocyte Growth Factor