Can apomyoglobin form a complex with a spherical ligand? Interactions between apomyoglobin and [C60] fullerene derivative.
The preparation and characterization of the stable equine skeletal muscle apomyoglobin and eee-isomer of tris-malonic acid [C60] fullerene complex is reported. For this new bio-nanomaterial preparation, a procedure of complexation-during-protein-refolding was used and the obtained compound sustained separation by gel-filtration and ion-exchange chromatography. The apomyoglobin-tris-malonic acid [C60] fullerene complex was characterized by UV-vis spectroscopy, steady state fluorescence, time-resolved fluorescence, and circular dichroism spectroscopies. Important information provided by this study, regarding the stability and properties of new material, may lead to a better understanding of the apomyoglobin protein binding characteristics, as well as to development of novel antioxidant and photodynamic therapeutic agents and components for bioelectronic devices.
Kolsenik
Jenny
J
School of Chemistry, Raymond and Beverly Sackler Faculty of Exact Sciences, Tel Aviv University, Tel Aviv 69978, Israel.
Belgorodsky
Bogdan
B
Fadeev
Ludmila
L
Gozin
Michael
M
D000818:Animals; D000975:Antioxidants / Q000737:chemistry; D001059:Apoproteins / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D002942:Circular Dichroism; D037741:Fullerenes / Q000737:chemistry*; D006736:Horses; D008024:Ligands; D009211:Myoglobin / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D010778:Photochemotherapy / Q000295:instrumentation; D011485:Protein Binding; D017510:Protein Folding; D013056:Spectrophotometry, Ultraviolet
D000975:Antioxidants; D001059:Apoproteins; D037741:Fullerenes; D008024:Ligands; D009211:Myoglobin; C010857:apomyoglobin; C069837:fullerene C60