Experimental prediction of the wavelength-dependent path-length factor for optical intrinsic signal analysis.
Analysis of the optical intrinsic signal of an exposed cortex has been applied to measurement of functional brain activation. It is important for accurate measurement of concentration changes in oxygenated hemoglobin and deoxygenated hemoglobin to consider the wavelength dependence of the mean optical path lengths for the reflectance of cortical tissue. A method is proposed to experimentally estimate the wavelength dependence of the mean optical path length in cortical tissue from the multispectral reflectance of the exposed cortex without any additional instruments. The trend in the wavelength dependence of the mean optical path length estimated by the proposed method agrees with that estimated by the model-based prediction, whereas the magnitude of the wavelength dependence predicted by the proposed method is greater than that of the model-based prediction. The experimentally predicted mean optical path length minimizes the difference in the measured changes in the concentrations of the oxygenated hemoglobin and deoxygenated hemoglobin calculated from different wavelength pairs.
Sakaguchi
Koichiro
K
Department of Electronics and Electrical Engineering, Keio University, Yokohama, Japan. koichiro@okd.elec.keio.ac.jp
Tachibana
Tomoya
T
Furukawa
Shunsuke
S
Katsura
Takushige
T
Yamazaki
Kyoko
K
Kawaguchi
Hideo
H
Maki
Atsushi
A
Okada
Eiji
E
D000818:Animals; D001303:Auditory Cortex / Q000502:physiology*; D001931:Brain Mapping / Q000379:methods*; D003198:Computer Simulation; D005072:Evoked Potentials, Auditory / Q000502:physiology*; D006168:Guinea Pigs; D006454:Hemoglobins / Q000032:analysis*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008027:Light; D008297:Male; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D008959:Models, Neurological; D012542:Scattering, Radiation
D006454:Hemoglobins