Low-level laser irradiation (LLLI) promotes proliferation of mesenchymal and cardiac stem cells in culture.
OBJECTIVE
Low-level laser irradiation (LLLI) was found to promote the proliferation of various types of cells in vitro. Stem cells in general are of significance for implantation in regenerative medicine. The aim of the present study was to investigate the effect of LLLI on the proliferation of mesenchymal stem cells (MSCs) and cardiac stem cells (CSCs).


METHODS
Isolation of MSCs and CSCs was performed. The cells were cultured and laser irradiation was applied at energy densities of 1 and 3 J/cm2.


RESULTS
The number of MSCs and CSCs up to 2 and 4 weeks respectively, post-LLLI demonstrated a significant increase in the laser-treated cultures as compared to the control.


CONCLUSIONS
The present study clearly demonstrates the ability of LLLI to promote proliferation of MSCs and CSCs in vitro. These results may have an important impact on regenerative medicine.
Tuby
Hana
H
Department of Zoology, The George S. Wise Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel.
Maltz
Lidya
L
Oron
Uri
U
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects*; D002478:Cells, Cultured; D007150:Immunohistochemistry; D008297:Male; D059630:Mesenchymal Stem Cells / Q000528:radiation effects*; D032383:Myocytes, Cardiac / Q000528:radiation effects*; D051381:Rats