Direct DNA isolation from solid biological sources without pretreatments with proteinase-K and/or homogenization through automated DNA extraction.
Genomic DNA from solid biomaterials was directly isolated with an automated DNA extractor, which was based on magnetic bead technology with a bore-mediated grinding (BMG) system. The movement of the bore broke down the solid biomaterials, mixed crude lysates thoroughly with reagents to isolate the DNA, and carried the beads to the next step. The BMG system was suitable for the mechanical homogenization of the solid biomaterials and valid as an automated system for purifying the DNA from the solid biomaterials without the need for pretreatment or disruption procedures prior to the application of the solid biomaterials.
Ki
Jang-Seu
JS
Department of Life Science, College of Natural Sciences, Hanyang University, Seoul 133-791, Korea. kijs@hanyang.ac.kr
Chang
Ki Byum
KB
Roh
Hee June
HJ
Lee
Bong Youb
BY
Yoon
Joon Yong
JY
Jang
Gi Young
GY
D001331:Automation; D001483:Base Sequence; D001672:Biocompatible Materials / Q000737:chemistry; D001709:Biotechnology; D004247:DNA / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D019286:Endopeptidase K; D004867:Equipment Design; D008280:Magnetics; D010944:Plants / Q000235:genetics
D001672:Biocompatible Materials; D017931:DNA Primers; D004269:DNA, Bacterial; D018744:DNA, Plant; D004247:DNA; D019286:Endopeptidase K