title: Hair follicle regeneration using grafted rodent and human cells.
abstract: Hair follicle regeneration involves epithelial-mesenchymal interactions (EMIs) of follicular epithelial and dermal papilla (DP) cells. Co-grafting of those cellular components from mice allows complete hair reconstitution. However, regeneration of human hair in a similar manner has not been reported. Here, we investigated the possibility of cell-based hair generation from human cells. We found that DP-enriched cells (DPE) are more critical than epidermal cells in murine hair reconstitution on a cell number basis, and that murine DPE are also competent for hair regeneration with rat epidermal cells. Co-grafting of human keratinocytes derived from neonatal foreskins with murine DPE produced hair follicle-like structures consisting of multiple epidermal cell layers with a well-keratinized innermost region. Those structures expressed hair follicle-specific markers including hair keratin, and markers expressed during developmental stages. However, the lack of regular hair structures indicates abnormal folliculogenesis. Similar hair follicle-like structures were also generated with cultured human keratinocytes after the first passage, or with keratinocytes derived from adult foreskins, demonstrating that epidermal cells even at a mature stage can differentiate in response to inductive signals from DP cells. This study emphasizes the importance of EMI in follicular generation and the differentiation potential of epidermal keratinocytes.
authors: Life Science Research Center, Shiseido Research Center, Yokohama, Kanagawa, Japan.
authors: Ritsuko
authors: R
authors: Ehama
authors: Yumiko
authors: Y
authors: Ishimatsu-Tsuji
authors: Shunsuke
authors: S
authors: Iriyama
authors: Ritsuro
authors: R
authors: Ideta
authors: Tsutomu
authors: T
authors: Soma
authors: Kiichiro
authors: K
authors: Yano
authors: Chikako
authors: C
authors: Kawasaki
authors: Satoshi
authors: S
authors: Suzuki
authors: Yuji
authors: Y
authors: Shirakata
authors: Koji
authors: K
authors: Hashimoto
authors: Jiro
authors: J
authors: Kishimoto
mesh_terms: D000818:Animals; D004817:Epidermis / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology; D004848:Epithelium / Q000378:metabolism; D018859:Hair Follicle / Q000473:pathology* / Q000502:physiology*; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism; D007633:Keratins / Q000378:metabolism; D008648:Mesoderm / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D008819:Mice, Nude; D008822:Mice, Transgenic; D051381:Rats; D012038:Regeneration
chemical_list: D007633:Keratins