Metabolism of BTEX and naphtha compounds to methane in oil sands tailings.
Naphtha, comprising low molecular weight aliphatics and aromatics (C3-C14), is used as a diluent in processing of bitumen from oil sands. A small fraction (<1%) is lost to tailings waste and incorporated into mature fine tailings (MFT). BTEX (benzene, toluene, ethylbenzene, and xylenes) and whole naphtha were assessed for biodegradation under methanogenic conditions using MFT from an oil sands tailings settling basin. MFT spiked with 0.05-0.1% w/v of BTEX compounds produced up to 2.1 (+/-0.1) mmol of methane during 36 weeks of incubation. Metabolism of 0.5-1.0% w/v naphtha in MFT yielded up to 5.7 (+/-0.2) mmol of methane during 46 weeks of incubation. Gas chromatographic analyses showed that BTEX degraded in the sequence: toluene > o-xylene > m- plus p-xylene > ethylbenzene > benzene. Only 15-23% of whole naphtha, mainly n-alkanes (in the sequence: nonane > octane > heptane) and some BTEX compounds (toluene > o-xylene > m-xylene), was metabolized. Other naphtha constituents, such as iso-paraffins and naphthenes, remained unchanged during this period. These results suggest that the microbial communities in the MFT can readily utilize certain fractions of unrecovered naphtha in oil sands tailings and support methanogenesis in settling basins. Current study findings could influence extraction process, MFT management, and reclamation options.
Siddique
Tariq
T
Department of Biological Sciences, University of Alberta, Edmonton, AB T6G 2E9, Canada.
Fedorak
Phillip M
PM
MacKinnon
Michael D
MD
Foght
Julia M
JM
D000416:Alberta; D000473:Alkanes / Q000378:metabolism*; D001421:Bacteria, Anaerobic / Q000378:metabolism*; D001554:Benzene / Q000378:metabolism; D001555:Benzene Derivatives / Q000378:metabolism; D001673:Biodegradation, Environmental; D002849:Chromatography, Gas; D045424:Complex Mixtures / Q000378:metabolism*; D019652:Extraction and Processing Industry; D006838:Hydrocarbons / Q000737:chemistry*; D008697:Methane / Q000378:metabolism*; D012822:Silicon Dioxide; D014050:Toluene / Q000378:metabolism; D014992:Xylenes / Q000378:metabolism
D000473:Alkanes; D001555:Benzene Derivatives; D045424:Complex Mixtures; D006838:Hydrocarbons; D014992:Xylenes; D014050:Toluene; D012822:Silicon Dioxide; C006647:asphalt; D001554:Benzene; C004912:ethylbenzene; C004544:naphtha; D008697:Methane