Determination of peptaibol trace amounts in marine sediments by liquid chromatography/electrospray ionization-ion trap-mass spectrometry.
Extraction followed by reverse phase liquid chromatography (LC)/electrospray ionization-ion trap-mass spectrometry (ESI-IT-MS) analysis has been successfully developed for the determination of peptaibols, fungal toxic metabolites, in marine sediments. Spiking experiments showed that the mean recovery of target compounds exceeded 85% at a spiking level of 10 ng/g of sediment (wet weight). Detection and quantification limits were 250 and 830 pg/g of sediment, respectively. The method developed constituted the first sensitive assay for quantification of peptaibol trace amounts in a natural environment. A concentration of 5 ng/g in sediment samples collected from Fier d'Ars was found.
Poirier
Laurence
L
Université de Nantes, Nantes Atlantique Universités, SMAB, EA2160, Faculté de pharmacie, 1 rue G. Veil-BP 53508, Nantes F-44000, France. laurence.poirier@univ-nantes.fr
Amiard
Jean-Claude
JC
Mondeguer
Florence
F
Quiniou
Françoise
F
Ruiz
Nicolas
N
Pouchus
Yves François
YF
Montagu
Monique
M
D000408:Alamethicin / Q000302:isolation & purification; D002138:Calibration; D002853:Chromatography, Liquid; D045424:Complex Mixtures / Q000737:chemistry; D004777:Environment; D019015:Geologic Sediments / Q000737:chemistry*; D054848:Peptaibols; D010455:Peptides / Q000032:analysis* / Q000302:isolation & purification; D015203:Reproducibility of Results; D012997:Solvents; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D045424:Complex Mixtures; D054848:Peptaibols; D010455:Peptides; D012997:Solvents; C116937:peptaibolin; D000408:Alamethicin