Urukthapelstatin A, a novel cytotoxic substance from marine-derived Mechercharimyces asporophorigenens YM11-542. I. Fermentation, isolation and biological activities.
Urukthapelstatin A, a novel cyclic peptide, was isolated from the cultured mycelia of marine-derived Thermoactinomycetaceae bacterium Mechercharimyces asporophorigenens YM11-542. The peptide was purified by solvent extraction, silica gel chromatography, ODS flash chromatography, and finally by preparative HPLC. Urukthapelstatin A dose-dependently inhibited the growth of human lung cancer A549 cells with an IC(50) value of 12 nM. Urukthapelstatin A also showed potent cytotoxic activity against a human cancer cell line panel.
Matsuo
Yoshihide
Y
Marine Biotechnology Institute Co. Ltd., Heita, Kamaishi, Iwate. yoshihide.matsuo@mbio.jp
Kanoh
Kaneo
K
Yamori
Takao
T
Kasai
Hiroaki
H
Katsuta
Atsuko
A
Adachi
Kyoko
K
Shin-Ya
Kazuo
K
Shizuri
Yoshikazu
Y
D000903:Antibiotics, Antineoplastic / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D005285:Fermentation; D016936:Gram-Positive Endospore-Forming Bacteria / Q000378:metabolism*; D006801:Humans; D007930:Leucine / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology; D013844:Thiazoles / Q000302:isolation & purification / Q000494:pharmacology; D014871:Water Microbiology
D000903:Antibiotics, Antineoplastic; D013844:Thiazoles; C521435:urukthapelstatin A; D007930:Leucine