Effect of monosodium glutamate on oxidative stress and apoptosis in rat thymus.
It has been demonstrated that administration of high concentrations of monosodium glutamate (MSG), induce oxidative stress in different organs, but not in thymus. In the present study we examined the role of oxidative stress in MSG-induced thymocyte apoptosis. MSG was administrated intraperitoneally (4 mg/g of body weight) for six consecutive days. Animals were sacrificed at 1st, 7th, and 15th day after last MSG dose. MSG administration to animals significantly increased apoptotic rate of thymocytes (P < 0.01), together with significant increase of malondialdehyde (MDA) level (P < 0.001) and xanthine oxidase (XO) activity (P < 0.01), in time dependent manner. Catalase activity, during examination period, was significantly decreased (0 < 0.01). Obtained results showed that MSG treatment induced oxidative stress in thymus, which may have an important role in thymocyte apoptosis induced by MSG.
Pavlovic
Voja
V
Institute of Physiology, Medical Faculty University of Nis, Bulevar dr Zorana Djindjica, Nis, Serbia. vojapav@yahoo.com
Pavlovic
Dusica
D
Kocic
Gordana
G
Sokolovic
Dusan
D
Jevtovic-Stoimenov
Tatjana
T
Cekic
Snezana
S
Velickovic
Dragana
D
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D005503:Food Additives / Q000494:pharmacology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012970:Sodium Glutamate / Q000494:pharmacology*; D013950:Thymus Gland / Q000187:drug effects* / Q000378:metabolism; D014969:Xanthine Oxidase / Q000378:metabolism
D005503:Food Additives; D008315:Malondialdehyde; D014969:Xanthine Oxidase; D012970:Sodium Glutamate