Determination of selenium by GFAAS in slurries of fish feces to estimate the bioavailability of this micronutrient in feed used in pisciculture.
This paper presents a simple, fast and sensitive method to determine selenium in samples of feces and of fish feed by graphite furnace atomic absorption spectrometry (GFAAS) through the direct introduction of slurries of the samples into the spectrometer's graphite tube. The limits of detection (LOD) and quantification (LOQ) calculated for 20 readings of the blank of the standard slurries (0.50% m/v of feces or feed devoid of selenium) were 0.31 microg l(-1) and 1.03 microg l(-1), respectively, for the standard feces slurries and 0.35 microg l(-1) and 1.16 microg l(-1), respectively, for the standard feed slurries. The proposed method was applied in studies of bioavailability of selenium in different fish feeds and the results proved consistent with that obtained from samples mineralized by acid digestion using the microwave oven.
Silva
Fábio A
FA
Faculdade de Medicina Veterinária e Zootecnia, Programa de Pós-Graduação em Zootecnia, Departamento de Melhoramento e Nutrição Animal/UNESP, Caixa Postal 560, 18618-000 Botucatu, SP, Brazil.
Neves
Renato C F
RC
Quintero-Pinto
Luis G
LG
Padilha
Cilene C F
CC
Jorge
Sônia M A
SM
Barros
Margarida M
MM
Pezzato
Luiz E
LE
Padilha
Pedro M
PM
D000821:Animal Feed / Q000032:analysis*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001682:Biological Availability; D005243:Feces / Q000737:chemistry*; D005398:Fisheries / Q000379:methods*; D005399:Fishes / Q000254:growth & development / Q000378:metabolism; D006108:Graphite; D012015:Reference Standards; D015203:Reproducibility of Results; D012643:Selenium / Q000032:analysis / Q000493:pharmacokinetics; D013054:Spectrophotometry, Atomic
D006108:Graphite; D012643:Selenium