[Expression of liposome-mediated PEDF-GFP gene in the retina of the BN rats through different gene delivery route].
OBJECTIVE
Transducing PEDF-GFP plasmid to the retina of the BN rats with cationic liposome through different gene delivery route, then observe the expression and location of the PEDF-GFP.


METHODS
PEDF-GFP plasmid with cationic liposome was delivered to the retina of the BN rat through subretinal injection and intravitreal injection. The expression of GFP was observed under fluorescence microscope, and the mRNA of PEDF gene was detected by RT-PCR.


RESULTS
Green fluorescence was emitted from the total retina include RPE cell under fluorescent microscope after 24 h in two gene delivery route, The fluorescence intensity was stronger with time changing. Gradual fluorescence increase in the retina and RPE cells occurred and lasted 4 weeks. The expression of PEDF mRNA was also detected by RT-PCR after 24 h, and maintained stable 4 weeks after infection.


CONCLUSIONS
Cationic liposome can mediate PEDF-GFP gene into the retina of the BN rat effectively; subretinal injection and intravitreal injection both are effective gene delivery route; and their stable expression can maintain 4 weeks after transfection.
Zhang
Xin-xiu
XX
Department of Ophthalmology, General Hospital of PLA, Beijing, China.
He
Shou-zhi
SZ
Guo
Wei-wei
WW
Zhai
Suo-qiang
SQ
D000818:Animals; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics; D008081:Liposomes; D008297:Male; D010957:Plasmids; D051381:Rats; D011914:Rats, Inbred BN; D018013:Receptors, Neuropeptide / Q000235:genetics*; D012160:Retina; D055213:Retinal Pigment Epithelium / Q000166:cytology; D014162:Transfection
D008081:Liposomes; D018013:Receptors, Neuropeptide; C400531:pigment epithelium-derived factor receptor; D049452:Green Fluorescent Proteins