On-capillary sample cleanup method for the electrophoretic determination of carbohydrates in juice samples.
On many occasions, sample treatment is a critical step in electrophoretic analysis. As an alternative to batch procedures, in this work, a new strategy is presented with a view to develop an on-capillary sample cleanup method. This strategy is based on the partial filling of the capillary with carboxylated single-walled carbon nanotube (c-SWNT). The nanoparticles retain interferences from the matrix allowing the determination and quantification of carbohydrates (viz glucose, maltose and fructose). The precision of the method for the analysis of real samples ranged from 5.3 to 6.4%. The proposed method was compared with a method based on a batch filtration of the juice sample through diatomaceous earth and further electrophoretic determination. This method was also validated in this work. The RSD for this other method ranged from 5.1 to 6%. The results obtained by both methods were statistically comparable demonstrating the accuracy of the proposed methods and their effectiveness. Electrophoretic separation of carbohydrates was achieved using 200 mM borate solution as a buffer at pH 9.5 and applying 15 kV. During separation, the capillary temperature was kept constant at 40 degrees C. For the on-capillary cleanup method, a solution containing 50 mg/L of c-SWNTs prepared in 300 mM borate solution at pH 9.5 was introduced for 60 s into the capillary just before sample introduction. For the electrophoretic analysis of samples cleaned in batch with diatomaceous earth, it is also recommended to introduce into the capillary, just before the sample, a 300 mM borate solution as it enhances the sensitivity and electrophoretic resolution.
Morales-Cid
Gabriel
G
Department of Analytical Chemistry, Marie Curie Building, Campus de Rabanales, University of Cordoba, Cordoba, Spain.
Simonet
Bartolomé M
BM
Cárdenas
Soledad
S
Valcárcel
Miguel
M
D002021:Buffers; D002241:Carbohydrates / Q000032:analysis*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D007692:Diatomaceous Earth / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D005374:Filtration / Q000379:methods; D005504:Food Analysis / Q000379:methods; D005638:Fruit / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D053758:Nanoparticles / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D002021:Buffers; D002241:Carbohydrates; D007202:Indicators and Reagents; D007692:Diatomaceous Earth