title: Determination of size and concentration of gold nanoparticles from UV-vis spectra.
abstract: The dependence of the optical properties of spherical gold nanoparticles on particle size and wavelength were analyzed theoretically using multipole scattering theory, where the complex refractive index of gold was corrected for the effect of a reduced mean free path of the conduction electrons in small particles. To compare these theoretical results to experimental data, gold nanoparticles in the size range of 5 to 100 nm were synthesized and characterized with TEM and UV-vis. Excellent agreement was found between theory and experiment. It is shown that the data produced here can be used to determine both size and concentration of gold nanoparticles directly from UV-vis spectra. Equations for this purpose are derived, and the precision of various methods is discussed. The major aim of this work is to provide a simple and fast method to determine size and concentration of nanoparticles.
authors: Centre for Nanoscale Science, Department of Chemistry, School of Biological Sciences, University of Liverpool, Crown Street L69 7ZD, UK. w.h.haiss@liv.ac.uk
authors: Wolfgang
authors: W
authors: Haiss
authors: Nguyen T K
authors: NT
authors: Thanh
authors: Jenny
authors: J
authors: Aveyard
authors: David G
authors: DG
authors: Fernig
mesh_terms: D004583:Electrons; D006046:Gold / Q000737:chemistry*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D013053:Spectrophotometry / Q000379:methods*; D020349:Surface Plasmon Resonance
chemical_list: D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D006046:Gold