title: Online preconcentration by transient isotachophoresis in linear polymer on a poly(methyl methacrylate) microchip for separation of human serum albumin immunoassay mixtures.
abstract: Online preconcentration of human serum albumin (HSA) and its immunocomplex with a monoclonal antibody by on-chip transient isotachophoresis is reported. An 800-fold signal enhancement was achieved following the preconcentration on standard cross-channel microchips made of poly (methyl methacrylate). Sample injection, preconcentration, and separation were done continuously and controlled solely by a sequential voltage switching program. The preconcentration was followed by on-chip nondenaturing gel electrophoresis in methylcellulose solution. The method was applied to microchip electrophoresis immunoassay of HSA. Baseline separation of HSA and its immunocomplex was achieved in 25 s in the first 1 cm of the microchannel. In a direct immunoassay, the minimum detectable concentration of fluorescent labeled HSA by laser-induced fluorescence detection was 7.5 pM.
authors: Department of Applied Chemistry, Graduate School of Engineering, MEXT Innovative Research Center for Preventive Medical Engineering, and Plasma Nanotechnology Research Center, Nagoya University, Nagoya 464-8603, Japan. rezam@mail.apchem.nagoya-u.ac.jp
authors: Mohamad Reza
authors: MR
authors: Mohamadi
authors: Noritada
authors: N
authors: Kaji
authors: Manabu
authors: M
authors: Tokeshi
authors: Yoshinobu
authors: Y
authors: Baba
mesh_terms: D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex / Q000302:isolation & purification; D046209:Electrophoresis, Microchip / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D011108:Polymers; D019904:Polymethyl Methacrylate; D012709:Serum Albumin / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D011108:Polymers; D012709:Serum Albumin; D019904:Polymethyl Methacrylate