title: Duodenal pain and spinal morphine induce conditioned taste aversion in rats.
abstract: Conditioned taste aversion (CTA) is a behavioural response essential to the survival of an individual. The combination of taste and odour of most foods provides a strong conditioned stimulus (CS) for an animal to respond in an appropriate way to any harmful unconditioned stimuli (US) that follow. The most widely used conditioned stimuli are drinkable sweet solutions, such as saccharin and sucrose. CTA-like responses are also found for environmental unconditioned stimuli, but these usually take longer training. In the present study, the aversive nature of a duodenal distention with an implanted balloon catheter was studied in freely moving rats using either CTA against a sucrose solution, or a light-dark passive avoidance (PA) paradigm. In addition, the effect of spinal morphine on CTA and the cardiovascular response to duodenal distention were studied. CTA could be induced by a single, but long-lasting 20-minute duodenal distention, which did not induce PA behaviour in a light-dark box. Spinal infusion of morphine alone induced CTA, suggesting that the model is unsuitable to investigate spinal pharmacological modulation of visceral pain. Spinal morphine did reduce the cardiovascular response to duodenal distention, strengthening its validity as a visceral pain model. Since CTA is a complicating factor in the field of chemotherapy in cancer patients and spinal morphine causes nausea and vomiting in humans, CTA may also complicate spinal drug treatment or anaesthesia.
authors: Department of Pharmacology and Anatomy, Rudolf Magnus Institute of Neuroscience, University Medical Center Utrecht, Utrecht, The Netherlands.
authors: Gert-Jan
authors: GJ
authors: Geerse
authors: Léon C A
authors: LC
authors: van Gurp
authors: Diane C W A
authors: DC
authors: van Wijk
authors: Victor M
authors: VM
authors: Wiegant
authors: Rianne
authors: R
authors: Stam
mesh_terms: D000818:Animals; D001245:Association Learning / Q000187:drug effects* / Q000502:physiology; D001362:Avoidance Learning / Q000187:drug effects* / Q000502:physiology; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D003214:Conditioning, Classical / Q000187:drug effects* / Q000502:physiology; D004106:Dilatation / Q000009:adverse effects; D004386:Duodenum / Q000503:physiopathology; D006339:Heart Rate / Q000187:drug effects / Q000502:physiology; D007278:Injections, Spinal; D008297:Male; D009020:Morphine / Q000008:administration & dosage / Q000009:adverse effects*; D009294:Narcotics / Q000008:administration & dosage / Q000009:adverse effects*; D010146:Pain / Q000188:drug therapy / Q000209:etiology / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar; D013649:Taste / Q000502:physiology
chemical_list: D009294:Narcotics; D009020:Morphine