title: Experimental infection with Haemophilus ducreyi in persons who are infected with HIV does not cause local or augment systemic viral replication.
abstract: We infected 11 HIV-seropositive volunteers whose CD4(+) cell counts were >350 cells/ microL (7 of whom were receiving antiretrovirals) with Haemophilus ducreyi. The papule and pustule formation rates were similar to those observed in HIV-seronegative historical control subjects. No subject experienced a sustained change in CD4(+) cell count or HIV RNA level. The cellular infiltrate in biopsy samples obtained from the HIV-seropositive and HIV-seronegative subjects did not differ with respect to the percentage of leukocytes, neutrophils, macrophages, or T cells. The CD4(+):CD8(+) cell ratio in biopsy samples from the HIV-seropositive subjects was 1:3, the inverse of the ratio seen in the HIV-seronegative subjects (P<.0001). Although CD4(+) cells proliferated in lesions, in situ hybridization and reverse-transcription polymerase chain reaction for HIV RNA was negative. We conclude that experimental infection in HIV-seropositive persons is clinically similar to infection in HIV-seronegative persons and does not cause local or augment systemic viral replication. Thus, prompt treatment of chancroid may abrogate increases in viral replication associated with natural disease.
authors: Department of Medicine, Indiana University, School of Medicine, Indianapolis, IN 46202, USA. dmjanowi@iupui.edu
authors: Diane M
authors: DM
authors: Janowicz
authors: Klara
authors: K
authors: Tenner-Racz
authors: Paul
authors: P
authors: Racz
authors: Tricia L
authors: TL
authors: Humphreys
authors: Carol
authors: C
authors: Schnizlein-Bick
authors: Kate R
authors: KR
authors: Fortney
authors: Beth
authors: B
authors: Zwickl
authors: Barry P
authors: BP
authors: Katz
authors: James J
authors: JJ
authors: Campbell
authors: David D
authors: DD
authors: Ho
authors: Stanley M
authors: SM
authors: Spinola
mesh_terms: D000328:Adult; D023241:Antiretroviral Therapy, Highly Active; D018791:CD4 Lymphocyte Count; D002602:Chancroid / Q000097:blood / Q000150:complications* / Q000473:pathology; D005260:Female; D015658:HIV Infections / Q000097:blood / Q000150:complications* / Q000188:drug therapy; D006191:Haemophilus ducreyi / Q000502:physiology*; D006801:Humans; D007958:Leukocyte Count; D018655:Lymphocyte Count; D008297:Male; D012367:RNA, Viral / Q000097:blood; D019562:Viral Load; D014779:Virus Replication
chemical_list: D012367:RNA, Viral