title: Efficient modification on PLLA by ozone treatment for biomedical applications.
abstract: RGDS (Arg-Gly-Asp-Ser) is immobilized on poly(L-lactic acid) (PLLA) with ozone oxidation and the addition of an intermediate reactant, acryl succinimide (ASI) to promote the grafting efficiency. A DPPH (2,2-di(4-tert-octylphenyl)-1-picrylhydrazyl) assay has revealed that the peroxide concentration can be controlled by adjusting the ozone treatment time. The immobilization of ASI is verified by elemental analysis. The peptide concentrations are in the effective order, as shown by means of high performance liquid chromatography (HPLC), and the grafting efficiency is proven to be relatively high compared with the previous studies. The culture of rat osteosarcoma 17/2.8 (ROS), osteoblastic-like cells, demonstrates that the grafting of RGDS can enhance the attachment and osteogenesis of ROS cells on PLLA. With the addition of ASI, the cultured ROS cells express normal function in proliferation and mineralization. From in vivo experiments, ASI immobilized on the surface is shown to be biocompatible. These results lead to the conclusion that the ozone treatment with the intermediate reactant ASI is an efficient, biocompatible, and easily controllable procedure to modify PLLA. Furthermore, the immobilization of RGDS in significant amounts following the ozone oxidation could further promote the biocompatibility and the osteoinduction of PLLA.
authors: R&D Center of Membrane Technology and Department of Chemical Engineering, Chung Yuan University, Chungli, Taiwan ROC. mhho@mail.ntust.edu.tw
authors: Ming-Hua
authors: MH
authors: Ho
authors: Juin-Jay
authors: JJ
authors: Lee
authors: Shu-Chin
authors: SC
authors: Fan
authors: Da-Ming
authors: DM
authors: Wang
authors: Lein-Tuan
authors: LT
authors: Hou
authors: Hsyue-Jen
authors: HJ
authors: Hsieh
authors: Juin-Yih
authors: JY
authors: Lai
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D002113:Calcification, Physiologic / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D019344:Lactic Acid / Q000737:chemistry / Q000494:pharmacology*; D008422:Materials Testing; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology*; D010006:Osteoblasts / Q000378:metabolism*; D010012:Osteogenesis / Q000187:drug effects*; D010084:Oxidation-Reduction; D010126:Ozone / Q000737:chemistry*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D013388:Succinimides / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D009842:Oligopeptides; D011091:Polyesters; D011108:Polymers; D013388:Succinimides; C032620:succinimide; D019344:Lactic Acid; C033616:poly(lactide); D010126:Ozone; C046617:arginyl-glycyl-aspartyl-serine