Cloning and characterization of a novel Delta12-fatty acid desaturase gene from the tree Sapium sebiferum.
A new full-length cDNA (Ssd12) encoding a Delta12-fatty acid desaturase (Delta12-FAD) was cloned from Sapium sebiferum using RT-PCR and RACE methods. Ssd12 contained a 1146 bp open reading frame encoding a protein of 381 amino acids. The amino acid sequence showed a much higher match with microsomal Delta12-FAD amino acid sequences than chloroplast Delta12-FAD amino acid sequences. Genomic Southern blot analysis suggested that Ssd12 had at least two copies. Ssd12 transcripts were detected in roots, leaves, stems, and seeds by real time PCR.
Niu
Bei
B
College of Life Science, Sichuan University, Chengdu, China.
Ye
Huaxun
H
Xu
Ying
Y
Wang
Shenghua
S
Chen
Peng
P
Peng
Shuming
S
Ou
Yangchao
Y
Tang
Lin
L
Chen
Fang
F
D000595:Amino Acid Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D044943:Fatty Acid Desaturases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D020869:Gene Expression Profiling; D018745:Genome, Plant; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics; D018547:Plant Stems / Q000201:enzymology / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D031287:Sapium / Q000201:enzymology / Q000235:genetics*; D012639:Seeds / Q000201:enzymology / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D005227:Fatty Acids; D010940:Plant Proteins; D011994:Recombinant Proteins; D044943:Fatty Acid Desaturases