In vitro effects of growth factors and interferon-alpha on busulfan cytotoxicity.
An experimental approach to increasing the effectiveness of leukemia treatment with S-phase-specific cytotoxics is to increase the cycling of leukemia cells with growth factors. However, growth factors may have a different relationship with non-cell-cycle-specific agents. The authors examined the effects of granulocyte colony-stimulating factor (G-CSF), granulocyte/macrophage colony-stimulating factor (GM-CSF), and interferon-alpha (INF-alpha) on the cytotoxic effects of the alkylating agent busulfan on the erythro-myeloid cell line K562. G-CSF and GM-CSF increased the proliferation and colony-forming ability of K562 cells and protected the cells from busulfan effects. INF-alpha decreased the colony-forming ability and proliferation of the K562 cells and demonstrated a possibly additive effect with busulfan. In the cell line K562, the growth factors G-CSF and GM-CSF protected the cells from the non-cell-cycle-specific alkylating agent busulfan, whereas IFN-alpha demonstrated an additive cytotoxic effect.
Ertan
Nesrin Zeynep
NZ
Istanbul Faculty of Medicine, Department of Physiology, Istanbul University, Capa, Istanbul, 34390, Turkey.
Erdem-Kuruca
Serap
S
Akgun-Dar
Kadriye
K
D018906:Antineoplastic Agents, Alkylating / Q000633:toxicity*; D002066:Busulfan / Q000633:toxicity*; D049109:Cell Proliferation / Q000187:drug effects*; D003114:Colony-Forming Units Assay; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology*; D020014:K562 Cells / Q000187:drug effects
D018906:Antineoplastic Agents, Alkylating; D016898:Interferon-alpha; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D002066:Busulfan