Trolox protection of myelin membrane in hydrogen peroxide-treated mature oligodendrocytes.
Oligodendrocytes have the highest rate of metabolic activity in the brain and are highly vulnerable to oxidative stress. In this work we determined the protective effect of Trolox, a water-soluble analogue of vitamin E, and insulin, a peptide shown to be neuroprotective, in oligodendrocyte lesion induced by hydrogen peroxide (H(2)O(2)). Exposure of primary cultures of rat oligodendrocytes to H(2)O(2) dose-dependently decreased their reducing capacity, as determined by the MTT assay. H(2)O(2) (100 muM) had no effect on Bax levels, active-caspase-3, DNA fragmentation or lactate dehydrogenase (LDH) leakage. Nevertheless, under these conditions, H(2)O(2) decreased the levels of myelin basic protein (MBP), used as a marker for oligodendrocyte myelin membrane. Treatment with insulin alone increased MBP levels, but no changes were observed in the presence of insulin plus H(2)O(2). In contrast, incubation with Trolox completely prevented H(2)O(2)-induced decrease in MBP expression, suggesting that vitamin E analogues may prevent against oligodendrocyte oxidative damage.
Perfeito
Rita
R
Faculty of Medicine, Center for Neuroscience and Cell Biology and Institute of Biochemistry, University of Coimbra, Coimbra, Portugal.
Pereira
Jorge
J
Oliveira
Catarina R
CR
Bettencourt-Relvas
João
J
Rego
A Cristina
AC
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D002470:Cell Survival; D002839:Chromans / Q000494:pharmacology*; D053938:DNA Fragmentation; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology*; D007328:Insulin / Q000737:chemistry; D004676:Myelin Basic Protein / Q000737:chemistry; D009186:Myelin Sheath / Q000737:chemistry*; D009836:Oligodendroglia / Q000378:metabolism*; D018384:Oxidative Stress; D051381:Rats; D014867:Water / Q000737:chemistry
D000975:Antioxidants; D002839:Chromans; D007328:Insulin; D004676:Myelin Basic Protein; D014867:Water; D006861:Hydrogen Peroxide; D053148:Caspase 3; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid