[Establishment of urinary proteomic map on two-dimensional polyacrylamide gel electrophoresis in children with idiopathic nephrotic syndrome].
OBJECTIVE
To establish a urinary proteomic map on two-dimensional polyacrylamide gelelectrophoresis (2-DE) in children with idiopathic nephrotic syndrome (INS).


METHODS
The proteins from INS children were purified by four various means, separated by 2-DEand stained by silver.


RESULTS
The sequential preparation of urinary proteins by acetone precipitation and dislysis, when the sample was 300 microg, resulted in a clear background, well-resolved and reproducible 2-DE urinary protemic map in children with INS.


CONCLUSIONS
A steady 2-DE technique for urinary protemic map in children with INS was established, which can be effectively applied in urinary proteomics of the disease.
Ding
Juan-Juan
JJ
Department of Pediatrics, Second Xiangya Hospital, Central South University, Changsha, China.
He
Qing-Nan
QN
Zhou
Pin
P
Zhang
Lei
L
Yi
Zhu-Wen
ZW
D002648:Child; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008297:Male; D009404:Nephrotic Syndrome / Q000652:urine*; D011507:Proteinuria / Q000652:urine*; D040901:Proteomics / Q000379:methods*