title: Comparative proteomic analysis of human pancreatic juice: methodological study.
abstract: Pancreatic cancer is the most lethal of all the common malignancies. Markers for early detection of this disease are urgently needed. Here, we optimized and applied a proteome analysis of human pancreatic juice to identify biomarkers for pancreatic cancer. Pancreatic juice samples, devoid of blood or bile contamination, were collected from patients with pancreatic cancer (n = 5), benign pancreatic diseases (n = 6), or cholelithiasis (n = 3) during endoscopic retrograde cholangiopancreatography (ERCP). After ultramembrane centrifugation sample preparation, pancreatic juice proteins were separated by 2-DE and identified by MALDI-TOF-MS. A 2-DE dataset of pancreatic juice from patients with cholelithiasis was established, consisting of 76 protein spots representing 22 different proteins. Disease-associated obstruction of the pancreatic duct strongly effected the protein composition of pancreatic juice. Concurrently, pancreatic juice from patients with pancreatic cancer was compared to nonmalignant controls with comparable obstruction of pancreatic ducts. Seven protein spots were identified that consistently appeared at changed levels in pancreatic juice from patients with pancreatic cancer. In conclusion, comparative proteomic analysis of human pancreatic juice can be used to identify biomarkers of pancreatic cancer.
authors: Department of Gastroenterology, Peking Union Medical College Hospital, Chinese Academy of Medical Science, Peking Union Medical College, Beijing, Peoples Republic of China.
authors: Lu
authors: L
authors: Zhou
authors: ZhaoHui
authors: Z
authors: Lu
authors: AiMing
authors: A
authors: Yang
authors: RuiXue
authors: R
authors: Deng
authors: CanRong
authors: C
authors: Mai
authors: XinTing
authors: X
authors: Sang
authors: Klaas Nico
authors: KN
authors: Faber
authors: XingHua
authors: X
authors: Lu
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010179:Pancreas / Q000033:anatomy & histology / Q000737:chemistry / Q000473:pathology; D010189:Pancreatic Juice / Q000737:chemistry*; D010190:Pancreatic Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D012633:Secretin / Q000737:chemistry
chemical_list: D014408:Biomarkers, Tumor; D020543:Proteome; D012633:Secretin