Melanoma-associated antigen family protein-D1 regulation of tumor cell migration, adhesion to endothelium, and actin structures reorganization in response to hypoxic stress.
Melanoma-associated antigen family protein-D1 (MAGE-D1) is a recently identified p75 neurotrophin receptor intracellular binding protein and functions as an adaptor that mediates multiple signaling pathways, including Dlx/Msx-mediated transcription. Here, a new regulatory function for MAGE-D1 in tumor cell motility and adhesion to endothelium is described. MAGE-D1 over-expression suppressed HeLa cell and BEL7402 cell migration, invasion, and adhesion to the monolayer of ECV304 cells. We also report that MAGE-D1 over-expression disrupted actin cytoskeleton rearrangement induced by hypoxia and down-regulated hypoxia inducible factor 1-dependent luciferase gene expression. These findings provide new insight into the ability of MAGE-D1 to suppress the motility and adhesion response of tumor cells by interfering with actin cytoskeleton reorganization and hypoxia inducible factor 1-dependent gene expression.
Shen
Wei-Gan
WG
Medical College of Yangzhou University, Yangzhou, China. weiganshen@yahoo.com.cn
Xue
Qing-Yu
QY
Wu
Yi-Ding
YD
Hu
Ben-Shun
BS
Zhu
Jun
J
Zhang
Yu
Y
Su
Qing
Q
D000199:Actins / Q000737:chemistry / Q000378:metabolism*; D000256:Adenoviridae / Q000235:genetics; D000951:Antigens, Neoplasm / Q000378:metabolism*; D015153:Blotting, Western; D002448:Cell Adhesion; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D003599:Cytoskeleton / Q000378:metabolism; D004727:Endothelium / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D006367:HeLa Cells; D006801:Humans; D051793:Hypoxia-Inducible Factor 1 / Q000235:genetics; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000378:metabolism*; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology*; D011554:Pseudopodia / Q000378:metabolism; D014945:Wound Healing
D000199:Actins; D000951:Antigens, Neoplasm; D051793:Hypoxia-Inducible Factor 1; C120897:MAGED1 protein, human; D009363:Neoplasm Proteins