title: The anatomy of the cerebellar nuclei in the normal and scrambler mouse as revealed by the expression of the microtubule-associated protein kinesin light chain 3.
abstract: Conventional kinesin is a motor protein complex including two heavy chains and two light chains (KLC). Junco et al. (Junco, A., Bhullar, B., Tarnasky, H.A. and van der Hoorn, F.A., 2001. Kinesin light-chain KLC3 expression in testis is restricted to spermatids. Biol. Reprod. 64, 1320-1330). recently reported the isolation of a novel KLC gene, klc3. In the present report, immunohistochemistry has been used to characterize the expression of KLC3 in the cerebella of normal and scrambler (scm) mutant mice. In cryostat sections through the cerebellum of the normal adult mouse immunoperoxidase stained for KLC3, reaction product is deposited in the nuclei and somata of deep cerebellar nuclear neurons. No other structures are stained in the cerebellum. Strong and specific KLC3 expression is observed in the adult cerebellum in all three major cerebellar nuclei--medial, interposed, and lateral. Double immunofluorescence studies reveal that KLC3 immunoreactivity is colocalized with both endosomes and GW bodies. KLC3 immunohistochemistry has been exploited to study the organization of the cerebellar nuclei in scrambler mice, in which disruption of the mdab1 gene results in severe foliation defects due to Purkinje cell ectopia, with most Purkinje cells clumped in centrally located clusters. Despite the severe failure of Purkinje cell migration, the cerebellar nuclei appear normal in scrambler mutant mice, suggesting that their topography is dependent neither on normal Purkinje cell positioning nor the Reelin signaling pathway.
authors: Department of Cell Biology and Anatomy, Hotchkiss Brain Institute, and Genes and Development Research Group, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada.
authors: Seunghyuk
authors: S
authors: Chung
authors: Ying
authors: Y
authors: Zhang
authors: Frans
authors: F
authors: Van Der Hoorn
authors: Richard
authors: R
authors: Hawkes
mesh_terms: D000367:Age Factors; D000818:Animals; D002529:Cerebellar Nuclei / Q000378:metabolism*; D015870:Gene Expression / Q000235:genetics / Q000502:physiology*; D016547:Kinesins; D051379:Mice; D008818:Mice, Neurologic Mutants / Q000033:anatomy & histology* / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D000091042:Reelin Protein
chemical_list: C000715693:Klc3 protein, mouse; D008869:Microtubule-Associated Proteins; D000091042:Reelin Protein; C000717128:Reln protein, mouse; D016547:Kinesins