Glycoconjugate histochemistry of the digestive tract of Triturus carnifex (Amphibia, Caudata).
In this study, the variety of sugar residues in the gut glycoconjugates of Triturus carnifex (Amphibia, Caudata) are investigated by carbohydrate conventional histochemistry and lectin histochemistry. The oesophageal surface mucous cells contained acidic glycoconjugates, with residues of GalNAc, Gal beta1,3 GalNAc and (GlcNAc beta1,4)(n) oligomers. The gastric surface cells mainly produced neutral glycoproteins with residues of fucose, Gal beta1-3 GalNAc, Gal-alphaGal, and (GlcNAc beta1,4)(n) oligomers in N- and O-linked glycans, as the glandular mucous neck cells, with residues of mannose/glucose, GalNAc, Gal beta1,3 GalNAc, (GlcNAc beta1,4)(n)oligomers and fucose linked alpha1,6 or terminal alpha1,3 or alpha1,4 in O-linked glycans. The oxynticopeptic tubulo-vesicular system contained neutral glycoproteins with N- and O-linked glycans with residues of Gal-alphaGal, Gal beta1-3 GalNAc and (GlcNAc beta1,4)(n)oligomers; Fuc linked alpha1,2 to Gal, alpha1,3 to GlcNAc in (poly)lactosamine chains and alpha1,6 to GlcNAc in N-linked glycans. Most of these glycoproteins probably corresponds to the H(+)K(+)-ATPase beta-subunit. The intestinal goblet cells contained acidic glycoconjugates, with residues of GalNAc, mannose/ glucose, (GlcNAc beta1,4)(n)oligomers and fucose linked alpha1,2 to Gal in O-linked oligosaccharides. The different composition of the mucus in the digestive tracts may be correlated with its different functions. In fact the presence of abundant sulphation of glycoconjugates, mainly in the oesophagus and intestine, probably confers resistance to bacterial enzymatic degradation of the mucus barrier.
Liquori
Giuseppa Esterina
GE
Dipartimento di Zoologia, Laboratorio di Istologia e Anatomia comparata, Università degli Studi di Bari, via Orabona 4/a, Bari 70125, Italy. g.liquori@biologia.uniba.it
Mastrodonato
Maria
M
Zizza
Sara
S
Ferri
Domenico
D
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D041981:Gastrointestinal Tract / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D006001:Glycoconjugates / Q000737:chemistry* / Q000378:metabolism; D006651:Histocytochemistry; D037102:Lectins / Q000378:metabolism*; D014319:Triturus / Q000033:anatomy & histology / Q000378:metabolism
D006001:Glycoconjugates; D037102:Lectins