Cloning and expression of the XPR2 gene from Yarrowia lipolytica in Pichia pastoris.
Yarrowia lipolytica is a dimorphic yeast able to secrete different types of proteases depending on the pH of the environment. At neutral pH, the production of an extracellular alkaline protease (AEP) is induced. This protease could be useful in the leather, detergent, or food industries. The XPR2 gene, coding for AEP, was extracted from the pINA154 vector and cloned into the pHIL-D2 vector to obtain a new protease-producing recombinant Pichia pastoris strain. The gene was efficiently integrated in the P. pastoris genome and expressed from the AOX1 promoter actively induced by methanol. Finally, the protease was successfully secreted by P. pastoris GS115.
Poza
M
M
Department of Microbiology, Faculty of Pharmacy, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain.
Sestelo
A B F
AB
Ageitos
J M
JM
Vallejo
J A
JA
Veiga-Crespo
P
P
Villa
T G
TG
D001426:Bacterial Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D010450:Endopeptidases / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D010843:Pichia / Q000235:genetics*; D011994:Recombinant Proteins; D025062:Yarrowia / Q000201:enzymology / Q000235:genetics*
D001426:Bacterial Proteins; D011994:Recombinant Proteins; D010450:Endopeptidases; C087822:alkaline protease