Vertically aligned carbon nanotubes as cytocompatible material for enhanced adhesion and proliferation of osteoblast-like cells.
In this study, we describe the spatial organization of CAL-72 osteoblast-like cells on arrays of vertically aligned multi-walled carbon nanotubes (VACNTs). It was observed that, unlike cell growth on non-patterned surfaces, the cell attachment and spreading process on VACNTs was significantly enhanced. Additionally, since carbon nanotubes are known to possess resilient mechanical properties and are chemically stable, the effect of periodic arrays of VACNTs on CAL-72 osteoblast-like cells was also studied. The periodicity and alignment of VACNTs considerably influenced growth, shape and orientation of the cells by steering toward the nanopattern. This situation is of great interest for the potential application of VACNTs in bone bioenginnering. This data provides evidence that CAL-72 osteoblast-like cells can sense physical features at the nanoscale. These results give a fascinating insight into the ways in which cell growth can be influenced by man-made nanostructures and could provide a framework for achieving controlled cell guidance with controlled organization and special physical properties.
Giannona
Suna
S
Center of Advanced European Studies and Research (CAESAR), Division Nanoparticle Technology, Ludwig-Erhard Allee 2, D-53175 Bonn, Germany.
Firkowska
Izabela
I
Rojas-Chapana
José
J
Giersig
Michael
M
D001672:Biocompatible Materials / Q000737:chemistry*; D001842:Bone and Bones / Q000378:metabolism; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D006801:Humans; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles; D036103:Nanotechnology / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology*
D001672:Biocompatible Materials; D037742:Nanotubes, Carbon