An experimentally derived database of candidate Ras-interacting proteins.
We used a TAP-tag approach to identify candidate binding proteins for the related Ras family GTPases: H-Ras, R-Ras, and Rap1A. Protein complexes were isolated from mouse fibroblasts, and component proteins were identified by a combination of nanoflow HPLC and tandem mass spectrometry. H-Ras was found to associate with numerous cytoskeletal proteins including talin-1. R-Ras and Rap1A each associated with various signaling molecules, many of which are membrane-associated. Thus, we have established the first database of potential Ras interactors in mammalian cells.
Goldfinger
Lawrence E
LE
Division of Rheumatology, Department of Medicine, University of California, San Diego, La Jolla, California 92093, USA. lgoldfinger@ucsd.edu
Ptak
Celeste
C
Jeffery
Erin D
ED
Shabanowitz
Jeffrey
J
Han
Jaewon
J
Haling
Jacob R
JR
Sherman
Nicholas E
NE
Fox
Jay W
JW
Hunt
Donald F
DF
Ginsberg
Mark H
MH
D000818:Animals; D016466:CHO Cells; D002846:Chromatography, Affinity / Q000379:methods*; D006224:Cricetinae; D003412:Cricetulus; D030562:Databases, Protein; D051379:Mice; D041681:NIH 3T3 Cells; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020827:rap1 GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D018631:ras Proteins / Q000235:genetics / Q000378:metabolism*
D011993:Recombinant Fusion Proteins; D020827:rap1 GTP-Binding Proteins; D018631:ras Proteins