New tensio-active molecules stabilize a human G protein-coupled receptor in solution.
Structural characterization of membrane proteins is hampered by the instability of the isolated proteins in detergent solutions. Here, we describe a new class of phospholipid-like surfactants that stabilize the G protein-coupled receptor, BLT1. These compounds, called C(13)U(9), C(13)U(19), C(15)U(25) and C(17)U(16), were synthesized by radical polymerization of Tris(hydroxymethyl) acrylamidomethane in the presence of thioglycerol, first endowed with two hydrocarbon chains with variable lengths (13-17 carbon atoms), as transfer reagent. C(13)U(19), C(17)U(16) or C(15)U(25) significantly enhanced the stability of BLT1 in solution compared to what was obtained with common detergents. These molecules therefore represent a promising step towards the structural characterization of BLT1 and possibly other membrane proteins.
Damian
Marjorie
M
Institut des Biomolécules Max Mousseron, UMR 5247 CNRS-Universités Montpellier I et II, Faculté de Pharmacie, 15 Av. Ch. Flahault, BP 14491, 34093 Montpellier Cedex 5, France.
Perino
Sandrine
S
Polidori
Ange
A
Martin
Aimée
A
Serre
Laurence
L
Pucci
Bernard
B
Banères
Jean-Louis
JL
D000178:Acrylamides / Q000737:chemistry; D019281:Dimerization; D006801:Humans; D007975:Leukotriene B4 / Q000378:metabolism; D008024:Ligands; D017510:Protein Folding; D018102:Receptors, Leukotriene B4 / Q000737:chemistry* / Q000378:metabolism*; D012996:Solutions / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry; D013816:Thermodynamics; D013997:Time Factors
D000178:Acrylamides; C513240:LTB4R protein, human; D008024:Ligands; D018102:Receptors, Leukotriene B4; D012996:Solutions; D013501:Surface-Active Agents; C445419:tris(hydroxymethyl)-acrylamidomethane; D007975:Leukotriene B4