title: Integration of active human beta-galactosidase gene (100 kb) into genome using HSV/AAV amplicon vector.
abstract: Vectors based on herpes simplex virus type-1 (HSV-1) permit delivery of transgenes of up to 150 kb, while the inverted terminal repeats and Rep of the adeno-associated virus (AAV) can confer site-specific integration into the AAVS1 site, which allows sustained expression of a transgene. In this study, combination of the viral elements in HSV/AAV hybrid vectors has been applied for the infectious transfer of the human lysosomal beta-galactosidase (BGAL) gene of 100 kb. Temporary expression and functional activity of beta-galactosidase (beta-gal) could be detected in human beta-gal-deficient patient and glioblastoma (Gli36) cells upon infection with the basic BGAL amplicon vector. Sustained expression of beta-gal was achieved in Gli36 cells infected with rep-plus, but not rep-minus, HSV/AAV hybrid vectors. None of five clones isolated after rep-minus hybrid vector infection showed elevated beta-gal activity or site-specific integration. In contrast, 80% of the rep-plus clones possessed beta-gal activity at least twofold greater than normal levels for up to 4 months of continuous growth, and 33% of the clones exhibited AAVS1-specific integration of the ITR-flanked transgene. One of the rep-plus clones displayed integration of the ITR cassette only at the AAVS1 site, with no sequences outside the cassette detectable and beta-gal activity fourfold above normal levels. These data demonstrate AAVS1-specific integration of an entire genomic locus and expression of the transgene from the endogenous promoter mediated by an HSV/AAV hybrid vector.
authors: Molecular Neurogenetics Unit, Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
authors: A
authors: A
authors: Oehmig
authors: M L
authors: ML
authors: Cortés
authors: K F
authors: KF
authors: Perry
authors: M
authors: M
authors: Sena-Esteves
authors: Cornel
authors: C
authors: Fraefel
authors: X O
authors: XO
authors: Breakefield
mesh_terms: D000818:Animals; D015139:Blotting, Southern / Q000379:methods; D002461:Cell Line, Transformed; D002522:Chlorocebus aethiops; D000229:Dependovirus / Q000235:genetics*; D005347:Fibroblasts / Q000821:virology; D016537:Gangliosidosis, GM1 / Q000628:therapy; D015870:Gene Expression; D005818:Genetic Engineering; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000008:administration & dosage* / Q000235:genetics; D016678:Genome; D049452:Green Fluorescent Proteins / Q000235:genetics; D018259:Herpesvirus 1, Human / Q000235:genetics*; D006801:Humans; D013997:Time Factors; D014161:Transduction, Genetic / Q000379:methods; D019076:Transgenes; D016662:Virus Integration; D001616:beta-Galactosidase / Q000032:analysis / Q000235:genetics* / Q000378:metabolism
chemical_list: D049452:Green Fluorescent Proteins; D001616:beta-Galactosidase