Effect of astragaloside on cardiomyocyte apoptosis in murine coxsackievirus B3 myocarditis.
Astragaloside is the major component of Astragalus membranaceus, one of the Chinese medical herbs, and has several pharmacological actions on cardiovascular system, including positive inotropic, anti-arrhythmia and anti-oxidant activities. We have investigated the effect of astragaloside on cardiomyocyte apoptosis and expression of apoptosis-associated genes in mice with coxsackievirus B(3) (CVB(3))-induced myocarditis, the former of which has been detected by terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay and the latter determined by using a focused microarray. Results showed that cardiomyocyte apoptosis and expression of various apoptosis associated genes are inhibited after treatment with astragaloside. The anti-apoptotic activity of astragaloside may contribute to the improvement of clinical outcomes in treating myocarditis with pharmaceutics of Astragalus membranaceus.
Zhang
Z-C
ZC
Zhejiang Hospital, 12 Ling Yin Road, Hangzhou, Zhejiang Province 310013, China
Li
S-J
SJ
Yang
Y-Z
YZ
Chen
R-Z
RZ
Ge
J-B
JB
Chen
H-Z
HZ
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D003384:Coxsackievirus Infections / Q000188:drug therapy*; D004770:Enterovirus / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D009205:Myocarditis / Q000188:drug therapy* / Q000821:virology; D032383:Myocytes, Cardiac / Q000187:drug effects*; D012503:Saponins / Q000494:pharmacology* / Q000627:therapeutic use; D014315:Triterpenes / Q000494:pharmacology* / Q000627:therapeutic use
D012503:Saponins; D014315:Triterpenes; C052064:astragaloside A