Curcuma longa extract as a histological dye for collagen fibres and red blood cells.
Crude ethanolic extract and column chromatographic fractions of the Allepey cultivar of Curcuma longa Roxb, commonly called turmeric (tumeric) in commerce, were used as a stain for tissue sections. Staining was carried out under basic, acidic and neutral media conditions. Inorganic and organic dissolution solvents were used. The stain was used as a counterstain after alum and iron haematoxylins. C. longa stained collagen fibres, cytoplasm, red blood cells and muscle cells yellow. It also stained in a fashion similar to eosin, except for its intense yellow colour. Preliminary phytochemical evaluation of the active column fraction revealed that it contained flavonoids, free anthraquinone and deoxy sugar. A cheap, natural dye can thus be obtained from C. longa.
Avwioro
O G
OG
Department of Histopathology, School of Medical Laboratory Science, Obafemi Awolowo University Teaching Hospital, Ile-Ife, Nigeria. avwiorog@yahoo.com
Onwuka
S K
SK
Moody
J O
JO
Agbedahunsi
J M
JM
Oduola
T
T
Ekpo
O E
OE
Oladele
A A
AA
D004396:Coloring Agents; D030024:Curcuma; D003593:Cytoplasm / Q000648:ultrastructure; D004912:Erythrocytes / Q000166:cytology*; D024022:Fibrillar Collagens / Q000648:ultrastructure*; D006801:Humans; D032342:Muscle Cells / Q000166:cytology; D010936:Plant Extracts; D013194:Staining and Labeling / Q000379:methods
D004396:Coloring Agents; D024022:Fibrillar Collagens; D010936:Plant Extracts; C012596:turmeric extract