Immune response to and faecal shedding of Mycobacterium avium ssp. paratuberculosis in young dairy calves, and the association between test results in the calves and the infection status of their dams.
The objectives of this study were to: (i) evaluate the results of an intradermal skin test, a modified IFN-gamma test, and a commercial ELISA in commercially raised dairy calves at 2, 4, 6 and 8 months of age relative to faecal shedding of Mycobacterium avium ssp. paratuberculosis (MAP); (ii) determine the proportion of 8-month-old calves shedding MAP in faeces as detected by culture and One Tube Nested Polymerase Chain Reaction (OTSN-PCR) and (iii) explore the association between results of tests described above in the calves and the Paratuberculosis (PTB) status of their dams as determined by faecal culture and/or serology. The study calves belonged to two dairy herds with different risk of exposure to MAP (high and low) and were enrolled based on their dam's ELISA results prior to calving. Approximately 3% of the calves were shedding MAP in faeces at 8 months of age. No agreement was observed among the evaluated immunity-based tests or between the immunity-based tests and the detection of MAP in faeces. Although no association was observed between the infection status of the dam and the results from the IFN-gamma and skin tests on the calves, there is an indication that calves born from dams that were faecal shedders might be at a higher risk of testing positive to the IFN-gamma test at 8 months of age. The disagreement among all tests evaluated in this calf cohort suggests that the detection of MAP infection in young stock requires the use of combined multiple tests. The early detection of PTB in calves is a challenge that requires further exploration of new methods to confirm infection status. These new testing methods should be both affordable and compatible with regular husbandry practices.
Antognoli
M C
MC
Animal Population Health Institute, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523-1681, USA. maria.antognoli@colostate.edu
Hirst
H L
HL
Garry
F B
FB
Salman
M D
MD
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000175:diagnosis / Q000635:transmission*; D015331:Cohort Studies; D015169:Colony Count, Microbial / Q000662:veterinary; D003612:Dairying / Q000379:methods; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005243:Feces / Q000382:microbiology*; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000097:blood; D007428:Intradermal Tests / Q000379:methods / Q000662:veterinary; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology* / Q000302:isolation & purification; D010283:Paratuberculosis / Q000097:blood / Q000175:diagnosis / Q000635:transmission*; D011634:Public Health; D012680:Sensitivity and Specificity
D007371:Interferon-gamma