Beta-lactam resistance in Haemophilus parasuis Is mediated by plasmid pB1000 bearing blaROB-1.
beta-Lactam resistance in Haemophilus parasuis is an emerging phenomenon that has not yet been characterized from a molecular perspective. Clinical high-level beta-lactam-resistant isolates from Spain bore a novel plasmid, pB1000, expressing a functionally active ROB-1 beta-lactamase. Pulsed-field gel electrophoresis was applied for the first time to H. parasuis and showed that beta-lactam resistance is due to clonal spread of a resistant strain, BB1018, bearing pB1000.
San Millan
Alvaro
A
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain.
Escudero
Jose Antonio
JA
Catalan
Ana
A
Nieto
Silvia
S
Farelo
Fidel
F
Gibert
Magdalena
M
Moreno
Miguel Angel
MA
Dominguez
Lucas
L
Gonzalez-Zorn
Bruno
B
D000818:Animals; D016521:Electrophoresis, Gel, Pulsed-Field; D006192:Haemophilus Infections / Q000382:microbiology / Q000662:veterinary; D044137:Haemophilus parasuis / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010957:Plasmids; D017422:Sequence Analysis, DNA; D013552:Swine / Q000382:microbiology; D013553:Swine Diseases / Q000382:microbiology; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism; D047090:beta-Lactams / Q000494:pharmacology*
D047090:beta-Lactams; C071165:beta-lactamse ROB-1; D001618:beta-Lactamases