title: Mitochondrial tRNA genes and flanking regions in sudden infant death syndrome.
abstract: OBJECTIVE
  Mitochondrial DNA (mtDNA) mutations have been proposed as a genetic risk factor for sudden infant death syndrome (SIDS). The aim of this study was to further investigate this issue, by sequencing the mitochondrial tRNA genes with flanking regions in SIDS cases and controls.
  
  
  METHODS
  The selected genes were investigated in 24 cases of SIDS and 10 controls, the method used were direct sequencing. In addition, the A10398G mutation in the ND3 gene was investigated in 220 SIDS cases, 26 cases of infectious death and 93 controls, using allele-specific PCR.
  
  
  RESULTS
  Mutations, recorded as differences from the revised Cambridge sequence, were found in 32 different sites in the coding regions investigated. There was no difference in mutation frequency between SIDS cases and controls, and no single mutation was found associated with SIDS.
  
  
  CONCLUSIONS
  The present study does not indicate an association between a specific mitochondrial tRNA gene mutation and SIDS, nor a higher mtDNA tRNA mutation frequency in SIDS cases than in controls.
authors: Institute of Forensic Medicine, University of Oslo, Oslo, Norway. s.h.opdal@medisin.uio.no
authors: Siri Hauge
authors: SH
authors: Opdal
authors: Ashild
authors: A
authors: Vege
authors: Marianne
authors: M
authors: Arnestad
authors: Musse Ahmed
authors: MA
authors: Musse
authors: Torleiv Ole
authors: TO
authors: Rognum
mesh_terms: D024509:3' Flanking Region / Q000235:genetics; D024506:5' Flanking Region / Q000235:genetics; D016022:Case-Control Studies; D005260:Female; D050259:Genes, Mitochondrial / Q000235:genetics*; D020022:Genetic Predisposition to Disease / Q000235:genetics; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D009154:Mutation / Q000235:genetics*; D012313:RNA / Q000235:genetics*; D000077278:RNA, Mitochondrial; D012343:RNA, Transfer / Q000235:genetics*; D013398:Sudden Infant Death / Q000235:genetics*
chemical_list: D000077278:RNA, Mitochondrial; D012313:RNA; D012343:RNA, Transfer