title: Acute changes in sputum IL-10 following underground exposure to diesel exhaust.
abstract: BACKGROUND
  Although exposure to diesel exhaust has been linked with adverse health effects, little is known about the acute effects of exposure in the underground workplace.
  
  
  METHODS
  Cross-shift spirometry and sputum induction were completed on twelve subjects associated with comminuted rock removal (mucking) operations in an underground copper mine using diesel powered and pneumatic equipment on separate days, and sputum collected on a baseline non-exposure day as well.
  
  
  RESULTS
  For diesel operations, elemental carbon exposure averaged 538 +/- 512 microg/m(3) during the 1-2 hour operations. Sputum interleukin-10 decreased with diesel exhaust using one ELISA assay (3.69 v. 2.32 pg/ml, p = 0.015), but increased when measured with a different ELISA kit (0.18 v. 0.59 pg/ml, p = 0.019), consistent with an overall decline in IL-10 protein concentration but an increase in the biologically active form. Sputum interleukin-6 decreased with exposure to diesel exhaust, although this change lost statistical significance when restricted to non-smokers. There were no significant changes in spirometry, interleukins 1beta, 4, and 8, tumor necrosis factor alpha or 8-hydroxy-2'-deoxyguanosine.
  
  
  CONCLUSIONS
  High levels of diesel exhaust can result in rapid changes in sputum IL-10, suggesting possible protein modification.
authors: University of Arizona, Tucson, Arizona 85724-5210, USA. jburgess@u.arizona.edu
authors: Jefferey L
authors: JL
authors: Burgess
authors: Joy E
authors: JE
authors: Fleming
authors: Emmanuel M
authors: EM
authors: Mulenga
authors: Arun
authors: A
authors: Josyula
authors: Tracy A
authors: TA
authors: Hysong
authors: Philip J
authors: PJ
authors: Joggerst
authors: Margaret
authors: M
authors: Kurzius-Spencer
authors: Hugh B
authors: HB
authors: Miller
mesh_terms: D000328:Adult; D000393:Air Pollutants / Q000633:toxicity*; D004784:Environmental Monitoring; D005260:Female; D006801:Humans; D019570:Inhalation Exposure; D016753:Interleukin-10 / Q000378:metabolism*; D008168:Lung / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D008906:Mining; D009504:Neutrophils / Q000187:drug effects / Q000473:pathology; D016273:Occupational Exposure; D013147:Spirometry / Q000379:methods; D013183:Sputum / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D001335:Vehicle Emissions / Q000633:toxicity*
chemical_list: D000393:Air Pollutants; C508609:IL10 protein, human; D001335:Vehicle Emissions; D016753:Interleukin-10