title: Agrobacterium rhizogenes-mediated transformation of soybean to study root biology.
abstract: This protocol is used to induce transgenic roots on soybean to study the function of genes required in biological processes of the root. Young seedlings with unfolded cotyledons are infected at the cotyledonary node and/or hypocotyl with Agrobacterium rhizogenes carrying the gene construct to be tested and the infection sites are kept in an environment of high humidity. When the emerged hairy roots can support the plants, the main roots are removed and the transgenic roots can be tested. Using this method, almost 100% of the infected plants form hairy roots within 1 month from the start of the experiments.
authors: ARC Centre of Excellence for Integrative Legume Research, The University of Queensland, St Lucia, Queensland 4072, Australia.
authors: Attila
authors: A
authors: Kereszt
authors: Dongxue
authors: D
authors: Li
authors: Arief
authors: A
authors: Indrasumunar
authors: Cuc D T
authors: CD
authors: Nguyen
authors: Sureeporn
authors: S
authors: Nontachaiyapoom
authors: Mark
authors: M
authors: Kinkema
authors: Peter M
authors: PM
authors: Gresshoff
mesh_terms: D005818:Genetic Engineering / Q000379:methods*; D018525:Germination; D018517:Plant Roots / Q000033:anatomy & histology / Q000235:genetics / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000033:anatomy & histology / Q000254:growth & development / Q000502:physiology; D012231:Rhizobium / Q000235:genetics*; D013025:Glycine max / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development; D014170:Transformation, Genetic