title: Time sequence of airway remodeling in a mouse model of chronic asthma: the relation with airway hyperresponsiveness.
abstract: During the course of establishing an animal model of chronic asthma, we tried to elucidate the time sequence of airway hyperresponsiveness (AHR), airway inflammation, airway remodeling, and associated cytokines. Seven-week-old female BALB/c mice were studied as a chronic asthma model using ovalbumin (OVA). After sensitization, mice were exposed twice weekly to aerosolized OVA, and were divided into three groups depending on the duration of 4 weeks, 8 weeks, and 12 weeks. At each time point, airway responsiveness, inflammatory cells, cytokines in bronchoalveolar lavage fluids (BALF), serum OVA-specific IgE, IgG1, IgG2a, and histological examination were carried out. AHR to methacholine, increased levels of OVA-specific IgG1 and IgG2a, and goblet cell hyperplasia were continuously sustained at each time point of weeks. In contrast, we observed a time-dependent decrease in serum OVA-specific IgE, BALF eosinophils, BALF cytokines such as IL-13, transforming growth factor-beta1, and a time-dependent increase in BALF promatrix metalloproteinase-9 and peribronchial fibrosis. In this OVA-induced chronic asthma model, we observed airway remodelings as well as various cytokines and inflammatory cells being involved in different time-dependent manners. However, increased airway fibrosis did not directly correlate with a further increase in airway hyperresponsiveness.
authors: Division of Pulmonology, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors: Seung Joon
authors: SJ
authors: Kim
authors: Chi Hong
authors: CH
authors: Kim
authors: Joong Hyun
authors: JH
authors: Ahn
authors: Myung Sook
authors: MS
authors: Kim
authors: Seok Chan
authors: SC
authors: Kim
authors: Sook Young
authors: SY
authors: Lee
authors: Soon Seog
authors: SS
authors: Kwon
authors: Young Kyoon
authors: YK
authors: Kim
authors: Kwan Hyoung
authors: KH
authors: Kim
authors: Hwa Sik
authors: HS
authors: Moon
authors: Jeong Sup
authors: JS
authors: Song
authors: Sung Hak
authors: SH
authors: Park
mesh_terms: D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000276:immunology*; D002908:Chronic Disease; D016207:Cytokines / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D008168:Lung / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin; D011014:Pneumonia / Q000139:chemically induced / Q000276:immunology*; D013997:Time Factors
chemical_list: D016207:Cytokines; D010047:Ovalbumin