Heavy ion microbeam irradiation induces ultrastructural changes in isolated single fibers of skeletal muscle.
The effects of heavy ion microbeams on muscle fibers isolated from mouse skeletal muscles were examined by electron microscopy. The plasma membranes of heavy ion beam-irradiated areas of muscle fibers showed irregular protrusions and invaginations. In the cytoplasm, an irregular distribution of microfilaments was found near the plasma membrane. Sarcoplasmic reticula in the irradiated regions showed a distended appearance with flocculent material within the lumen. These changes were seen as early as 2 min after irradiation, and persisted until as late as 22 min after irradiation. Many autophagic vacuoles could be seen at 7 min after irradiation. At 22 min, the vacuoles became more prominent and showed more variety. These observations suggest that heavy ion beam irradiation causes disruption of the cellular architecture and the autophagy is involved in removal of this disruption.
Hino
Mizuki
M
Department of Neuromuscular and Developmental Anatomy, Division of Bioregulatory Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
Wada
Seiichi
S
Tajika
Yuki
Y
Morimura
Yoshihiro
Y
Hamada
Nobuyuki
N
Funayama
Tomoo
T
Sakashita
Tetsuya
T
Kakizaki
Takehiko
T
Kobayashi
Yasuhiko
Y
Yorifuji
Hiroshi
H
D000818:Animals; D001343:Autophagy; D002478:Cells, Cultured; D005260:Female; D020450:Heavy Ions; D051379:Mice; D046529:Microscopy, Electron, Transmission; D018485:Muscle Fibers, Skeletal / Q000528:radiation effects* / Q000648:ultrastructure*; D018482:Muscle, Skeletal / Q000528:radiation effects / Q000648:ultrastructure