Application of liquid chromatography-mass spectrometry to measure the concentrations and study the synthesis of short chain fatty acids following stable isotope infusions.
A new method involving zinc sulphate deproteinization was developed to study short chain fatty acids (SCFA) production in the colon and subsequent occurrence of SCFA in blood. SCFA were baseline separated in a 30 min cycle using ion-exclusion chromatography and detected by mass spectrometry. Concentrations could be measured down to 10 microM and isotopomeric distributions could be assessed, enabling the conduction of tracer studies to study changes in SCFA synthesis. The applicability of the method was tested in an extensively characterized pig model yielding portal SCFA concentrations ranging from 70 microM (butyric acid) to 150 microM (propionic acid) to 440 microM (acetic acid) prior to butyrate tracer infusion, reaching butyric acid isotopic steady state within 2 h.
Meesters
R J W
RJ
Department of Surgery, Maastricht University, Nutrition and Toxicology Research Institute Maastricht, P.O. Box 616, 6200 MD Maastricht, The Netherlands. rjw.meesters@AH.unimaas.nl
van Eijk
H M H
HM
ten Have
G A M
GA
de Graaf
A A
AA
Venema
K
K
van Rossum
B E J
BE
Deutz
N E P
NE
D000818:Animals; D002850:Chromatography, Gel / Q000379:methods*; D005227:Fatty Acids / Q000138:chemical synthesis*; D007554:Isotopes; D013058:Mass Spectrometry / Q000379:methods*; D013552:Swine
D005227:Fatty Acids; D007554:Isotopes