Inflammation-specific T1 imaging using anti-intercellular adhesion molecule 1 antibody-conjugated gadolinium diethylenetriaminepentaacetic acid.
To examine inflammatory tissue, an initial and common symptom of various types of pathogenesis, we designed inflammation-targeted T(1) contrast agents prepared by bioconjugation of gadolinium diethylenetriaminepentaacetic acid (Gd-DTPA) with anti-intercellular adhesion molecule 1 (ICAM-1) antibody. The anti-ICAM-1 antibody was coupled with DTPA and was then conjugated with Gd. The specific binding of the Gd-DTPA-anti-ICAM-1 antibody complex to the ICAM-1-expressing cells was examined in the cultured endothelial cells where ICAM-1 expression was stimulated. Inflammation-specific T(1) imaging was then assessed using a mouse abscess model with the 1.5-Tesla module. The Gd-DTPA-anti-ICAM-1 antibody displayed increased r1, which was two times higher than that of Gd-DTPA and showed predominant binding to cultured endothelial cells, which expressed a high level of ICAM-1. Moreover, the inflammation-specific T(1) enhancement was imaged with the Gd-DTPA-anti-ICAM-1 antibody in the mouse acute inflammation model. The Gd-DTPA-anti-ICAM-1 antibody showed significantly increased vascular circulation time, which thereby offered a greater chance for its binding to the target cells. The Gd-DTPA-anti-ICAM-1 antibody displays a potential targeted T(1) contrast agent specific to the inflammatory tissue that expresses ICAM-1.
Choi
Kyu-Sil
KS
Department of Radiology and Institute for Radiological Imaging Science, Wonkwang University School of Medicine, Iksan, Jeonbuk, South Korea.
Kim
Sun-Hee
SH
Cai
Quan-Yu
QY
Kim
Soo-Yeon
SY
Kim
Hyang-Ok
HO
Lee
Hye-Jin
HJ
Kim
Eun-A
EA
Yoon
Seong-Eon
SE
Yun
Ki-Jung
KJ
Yoon
Kwon-Ha
KH
D000038:Abscess / Q000175:diagnosis; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000493:pharmacokinetics; D002478:Cells, Cultured; D003287:Contrast Media / Q000737:chemistry; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000737:chemistry; D019786:Gadolinium DTPA / Q000032:analysis / Q000737:chemistry / Q000493:pharmacokinetics; D006207:Half-Life; D007150:Immunohistochemistry; D007249:Inflammation / Q000175:diagnosis* / Q000382:microbiology; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D051381:Rats; D013211:Staphylococcus aureus
D000911:Antibodies, Monoclonal; D003287:Contrast Media; D018799:Intercellular Adhesion Molecule-1; D019786:Gadolinium DTPA