title: [Protective action of effective components of Huanglian Jiedu decoction on hypoxia and reoxygenation injury in cultured rat cerebral microvascular endothelial cells].
abstract: OBJECTIVE
  To investigate the protective effect of geniposide, baicalin and berberine for the rat cerebral microvascular endothelial cell.
  
  
  METHODS
  The model of hypoxia and reoxygenation injury in rat cerebral microvascular endothelial cells in vitro was established. Both normal and model cells were treated with geniposide (1.024, 0.512, 0.256, 0.128, 0.064, 0.032, 0.016, 0.008 micromol x mL(-1)), baicalin (0.224, 0.112, 0.056, 0.028, 0.014, 0.007, 0.003 micromol x mL(-1)) and berberine (0.192, 0.096, 0.048, 0.024, 0.012, 0.006, 0.003 micromol x mL(-1)). Cell activity was measured by methyl thiazolyl tetrazolium (MTT) test.
  
  
  RESULTS
  After hypoxia/hypoglycemia cultures for 4 hour and reoxygenation for 12 hour, geniposide (0.128, 0.064, 0.032 micromol x mL(-1)), baicalin (0.028, 0.014, 0.007 micromol x mL(-1)) and berberine (0.024, 0.012, 0.006 micromol x microL(-1) could protect the injuried cerebral microvascular endothelial cells.
  
  
  CONCLUSIONS
  Appropriate concentration of geniposide, baicalin and berberine, which are effective components of Huanglian Jiedu decoction, could protect the injuried cerebral microvascular endothelial cells.
authors: First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China.
authors: Zheng-zhong
authors: ZZ
authors: Yuan
authors: Ling-qun
authors: LQ
authors: Zhu
authors: He
authors: H
authors: Pang
authors: Ze-song
authors: ZS
authors: Shan
authors: Shuo-ren
authors: SR
authors: Wang
authors: Yong-hong
authors: YH
authors: Gao
authors: Fu-ling
authors: FL
authors: Niu
mesh_terms: D000818:Animals; D001599:Berberine / Q000302:isolation & purification / Q000494:pharmacology*; D015687:Cell Hypoxia; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000098:blood supply; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects*; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D039823:Iridoids / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology; D010100:Oxygen / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry; D011714:Pyrans / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D039823:Iridoids; D018696:Neuroprotective Agents; D011714:Pyrans; C045607:huang-lien-chieh-tu-tang; D001599:Berberine; C007835:geniposide; C038044:baicalin; D010100:Oxygen