[Fingerprints of different samples of processed Rhizoma Coptidi by HPLC].
OBJECTIVE
To set up the HPLC fingerprints of different samples of processed Rhizoma Coptidi (RC) in order to offer evidence for identifying different processed RC and preliminary discussion on processing mechanism.


METHODS
Seven different samples of processed RC were determined by HPLC and the results were analyzed by Hierarchical clustering and similarity evaluation system for chromatographic fingerprint of TCM.


RESULTS
The characteristic of common peaks for fingerprints of different samples of processed RC is distinct, but there are differences among various processed RC. The different samples of processed RC were classified into two and the result of hierarchical clustering is correlated with the property of traditional Chinese drug.


CONCLUSIONS
It is proved that the method which is convenient, rapid and repeat well can be used to identify different samples of processed RC. The change of property of different processed RC is related with processing adjuvant, the possible mechanism is that new components are produced or the contents of known components have changed due to the adjuvant during processing.
Liao
Qing-wen
QW
PLA Institute of Chinese Materia Medica, 302 Hospital of PLA, Beijing 100039, China.
Fan
Dong-li
DL
Xiao
Xiao-he
XH
Luo
Jie-ying
JY
Liu
Shao-gui
SG
Yan
Dan
D
Ma
Yong-gang
YG
D019342:Acetic Acid; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D016000:Cluster Analysis; D039323:Coptis / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D020939:Zingiber officinale; D006358:Hot Temperature; D010946:Plants, Medicinal / Q000737:chemistry; D027343:Rhizome / Q000737:chemistry*; D017673:Sodium Chloride, Dietary; D013678:Technology, Pharmaceutical / Q000379:methods*; D014920:Wine
D004365:Drugs, Chinese Herbal; D017673:Sodium Chloride, Dietary; D019342:Acetic Acid