title: Association between oxidative stress in pregnancy and preterm premature rupture of membranes.
abstract: BACKGROUND
  Premature rupture of membranes (PROM) is caused by collagen damage in the chorioamniotic sac leading to tearing. Reactive oxygen species (ROS) may be the cause of collagen damage. Isoprostanes (F(2)-IP) are produced by ROS attack on polyunsaturated fatty acids and are sensitive and specific biomarkers of lipid-peroxidation in vivo.
  
  
  OBJECTIVE
  To verify whether oxidative stress occurs in pregnancies associated with preterm PROM.
  
  
  METHODS
  F(2)-IPs were measured in amniotic fluid of 16 pregnancies with preterm PROM (Group II) and 97 without PROM (Group I).
  
  
  RESULTS
  F(2)-IP concentrations (pg/mL) were significantly higher in group II than group I (p<0.0001). The ROC curve showed a sensitivity of 100% and a specificity of 84.5% at a cut-off of 124.4 pg/mL.
  
  
  CONCLUSIONS
  An association exists between oxidative stress in pregnancy and preterm PROM. The detection of amniotic fluid F(2)-IP concentrations seems to be a reliable predictive index of risk of preterm PROM.
authors: Department of Pediatrics, Obstetrics and Reproductive Medicine, University of Siena, Policlinico Le Scotte, V le Bracci 36, Siena, Italy.
authors: Mariangela
authors: M
authors: Longini
authors: Serafina
authors: S
authors: Perrone
authors: Piero
authors: P
authors: Vezzosi
authors: Barbara
authors: B
authors: Marzocchi
authors: Antonio
authors: A
authors: Kenanidis
authors: Giovanni
authors: G
authors: Centini
authors: Lucia
authors: L
authors: Rosignoli
authors: Giuseppe
authors: G
authors: Buonocore
mesh_terms: D000293:Adolescent; D000328:Adult; D000653:Amniotic Fluid / Q000378:metabolism; D028441:F2-Isoprostanes / Q000378:metabolism; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007231:Infant, Newborn; D018384:Oxidative Stress; D011247:Pregnancy
chemical_list: D028441:F2-Isoprostanes