Introducing genetically encoded aldehydes into proteins.
Methods for introducing bioorthogonal functionalities into proteins have become central to protein engineering efforts. Here we describe a method for the site-specific introduction of aldehyde groups into recombinant proteins using the 6-amino-acid consensus sequence recognized by the formylglycine-generating enzyme. This genetically encoded 'aldehyde tag' is no larger than a His(6) tag and can be exploited for numerous protein labeling applications.
Carrico
Isaac S
IS
Department of Chemistry, Howard Hughes Medical Institute, B84 Hildebrand Hall, University of California, Berkeley, California 94720, USA.
Carlson
Brian L
BL
Bertozzi
Carolyn R
CR
D000409:Alanine / Q000031:analogs & derivatives / Q000235:genetics / Q000378:metabolism; D000447:Aldehydes / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D016384:Consensus Sequence; D017124:Conserved Sequence; D005998:Glycine / Q000031:analogs & derivatives / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016324:Sequence Tagged Sites; D013429:Sulfatases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D000447:Aldehydes; D010446:Peptide Fragments; D011506:Proteins; C524739:C(alpha)-formylglycine; D013429:Sulfatases; D000409:Alanine; D005998:Glycine