Characterisation of a novel recombination event in the norovirus polymerase gene.
This communication describes a novel recombination event in the norovirus genome. Similarity plot analysis of a nucleotide fragment (1003 bp) amplified from a norovirus positive clinical specimen (IrlN05771) identified a previously undescribed recombination point in the 3' region of the polymerase gene (nucleotide position 4889 bp). Nucleotide multiple alignments demonstrated that Irl05N771 shared 78.6% and 94% identity with all other Irish norovirus sequences before and after the recombination point, respectively and confirmed the sequence as a genogroup II/type 4 recombinant. Irl05N771 shared more identity with Asian norovirus sequences. This is the first description of recombination within the norovirus polymerase and highlights the continuous genetic evolution of noroviruses.
Waters
Allison
A
Centre for Research in Infectious Diseases (CRID), School of Medical and Medical Sciences, National Virus Reference Laboratory (NVRL), University College Dublin (UCD), Belfield, Dublin 4, Ireland.
Coughlan
Suzie
S
Hall
William W
WW
D004259:DNA-Directed DNA Polymerase / Q000235:genetics* / Q000378:metabolism; D005814:Genes, Viral / Q000235:genetics*; D029322:Norovirus / Q000201:enzymology* / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D011995:Recombination, Genetic / Q000235:genetics*
D004259:DNA-Directed DNA Polymerase