Effects of hydrated forms of C60 fullerene on amyloid 1-peptide fibrillization in vitro and performance of the cognitive task.
It has been shown for the first time by transmission electron microscopy that the hydrated fullerene C60 inhibited the fibrillization of amyloid-beta25-35 peptide. The fullerene affected the amyloid-beta25-35 assembly, manifesting its anti-amyloidogenic capacity. Our in vivo investigations demonstrated also that a single intracerebroventricular injection of the C60 hydrated fullerene at a dose of 7.2 nmol/ventricle significantly improved the performance of the cognitive task in control rats. The intracerebroventricular injection of the C60 hydrated fullerene (3.6 nmol/ventricle) prevented the impairment of performance of the cognitive task induced by amyloid-beta25-35 (22.5 nmol/ventricle). The results obtained may be useful in the development of therapy of Alzheimer's disease.
Podolski
I Ya
IY
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Russia.
Podlubnaya
Z A
ZA
Kosenko
E A
EA
Mugantseva
E A
EA
Makarova
E G
EG
Marsagishvili
L G
LG
Shpagina
M D
MD
Kaminsky
Yu G
YG
Andrievsky
G V
GV
Klochkov
V K
VK
D000544:Alzheimer Disease / Q000188:drug therapy; D000595:Amino Acid Sequence; D000682:Amyloid / Q000737:chemistry*; D016229:Amyloid beta-Peptides / Q000737:chemistry; D000818:Animals; D001522:Behavior, Animal; D001921:Brain / Q000473:pathology; D004195:Disease Models, Animal; D037741:Fullerenes / Q000737:chemistry* / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects*; D007858:Learning; D008297:Male; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
D000682:Amyloid; D016229:Amyloid beta-Peptides; D037741:Fullerenes; D010446:Peptide Fragments; D010455:Peptides; C074636:amyloid beta-protein (25-35); C069837:fullerene C60