Milk and serum J5-specific antibody responses, milk production change, and clinical effects following intramammary Escherichia coli challenge for J5 vaccinate and control cows.
Holstein dairy cows (four J5 vaccinates and four controls) selected for no recorded intramammary disease and low somatic cell count (SCC) during the previous lactation were challenged by intramammary infusion of Escherichia coli. Vaccination with J5 was at 8 weeks and again 4 weeks before the anticipated calving date. Cows were challenged at 8 to 16 days in milk (DIM). Shedding of E. coli in milk was significantly higher among controls than vaccinates (no shedding) from 6 h to 21 h postchallenge. From 21 h to 132 h postchallenge, SCC in challenged quarters of controls (5,429,000/ml) was significantly higher than that of vaccinates (490,000/ml). On the day after challenge, milk production in control cows was 8 kg less, while vaccinates gained 0.5 kg, a significant difference. In serum immediately prior to challenge, J5-specific immunoglobulin G1 (IgG1) was significantly higher, IgG2 was nearly significantly higher, and IgM was the same in J5 vaccinates relative to controls. Vaccinates had proportionally more IgG2 in serum postcalving and in the first 12 h following challenge and less IgG2 in milk 24 h after challenge than the controls, approaching statistical significance. The ratio of J5-specific IgG1 and IgG2 combined compared to IgM was significantly higher in vaccinates than in controls in prechallenge serum (ratios of 15.8 and 3.2, respectively) and milk (5.0 and 1.3, respectively). Cows with higher IgM titers in milk 12 h postchallenge produced significantly less milk. Vaccination with J5 was significantly associated with higher production of J5-specific IgG1 and IgG2 in early lactation, reduced SCC, faster clearance of E. coli from milk, and less milk production loss following intramammary challenge.
Wilson
David J
DJ
Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, New York 14850, USA. David.Wilson@usu.edu
Mallard
Bonnie A
BA
Burton
Jeanne L
JL
Schukken
Ynte H
YH
Gröhn
Yrjo T
YT
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000097:blood; D001835:Body Weight / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control; D002452:Cell Count / Q000662:veterinary; D004926:Escherichia coli / Q000276:immunology* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000517:prevention & control / Q000662:veterinary*; D005260:Female; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood; D007075:Immunoglobulin M / Q000032:analysis / Q000097:blood; D007279:Injections, Subcutaneous; D008321:Mammary Glands, Animal; D008414:Mastitis, Bovine / Q000382:microbiology / Q000517:prevention & control*; D008892:Milk / Q000166:cytology / Q000276:immunology / Q000382:microbiology; D011247:Pregnancy; D013997:Time Factors; D014611:Vaccination / Q000662:veterinary*
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M