title: Evaluation of Daphne genkwa diterpenes: fingerprint and quantitative analysis by high performance liquid chromatography.
abstract: Daphne genkwa contains a novel class of anticancer diterpene esters that inhibit DNA topoisomerase I. Fingerprint and quantitative analysis by HPLC were performed in order to characterise and evaluate D. genkwa. A standard fingerprint of Daphne diterpene esters from the root extract was first established by HPLC-UV, and the major peaks in the fingerprint profile were preliminarily determined using HPLC-MS. The principal Daphne diterpene esters, yuanhuacine (1), yuanhuadine (2), yuanhuajine (3) and yuanhuagine (4), were isolated and identified using a combination of UV, IR, MS, 1H-NMR and 13C-NMR spectral data. Quantitative analysis indicated that 1 was the principal component in the root, and that 2 was the major component in the buds. The average extraction rates of 1 and 2 were 0.0151 and 0.0033% (n=10) from the root, respectively, and 0.0020 and 0.0078% (n=3) from the buds, respectively.
authors: State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116012, People's Republic of China. zhangsx56@yahoo.com.cn
authors: Shixuan
authors: S
authors: Zhang
authors: Fenghong
authors: F
authors: Zhang
authors: Xiaona
authors: X
authors: Li
authors: Weiqun
authors: W
authors: Dong
authors: Luohan
authors: L
authors: Wen
authors: Shisheng
authors: S
authors: Wang
mesh_terms: D000465:Algorithms; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D032364:Daphne / Q000737:chemistry*; D004224:Diterpenes / Q000032:analysis* / Q000737:chemistry; D004952:Esters / Q000032:analysis; D013058:Mass Spectrometry; D018517:Plant Roots / Q000737:chemistry; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
chemical_list: D004224:Diterpenes; D004952:Esters