Liquid chromatographic method with amperometric detection to determine acteoside in rat blood and brain microdialysates and its application to pharmacokinetic study.
A simple and sensitive liquid chromatography with amperometric detection was developed for the first time to monitor the protein-unbound acteoside in the rat blood and brain microdialysate by microdialysis technique. Microdialysis samples without further cleanup procedures were directly injected into the HPLC and separated using a reversed-phase C18 column (150 mmx2 mm, i.d. 5 microm) maintained at ambient temperature and a mobile phase comprised of acetonitrile-50 mM monosodium phosphate (pH 2.8) (17:83, v/v) with a flow rate of 0.2 mL/min. Based on the experimental voltamogram, the applied potential was set at +0.9 V oxidative mode. The concentration-response relationship was linear (r2>0.99) over a concentration range of 5-500 ng/mL; method precision and accuracy fell within predefined limits (less than 20%). The developed method was applied to assess the pharmacokinetics of acteoside, and the results suggested that acteoside was fitted better by the two-compartmental model following a single intravenous injection of acteoside. Acteoside was unable to be detected in the brain dialysate. The distribution and elimination half-lives of unbound acteoside in the blood were 5 and 28 min, respectively, which suggested the rapid distribution of acteoside.
Wu
Yu-Tse
YT
Institute of Traditional Medicine, National Yang-Ming University, Taipei 112, Taiwan.
Tsai
Tong-Rong
TR
Lin
Lie-Chwen
LC
Tsai
Tung-Hu
TH
D000818:Animals; D000890:Anti-Infective Agents / Q000097:blood / Q000493:pharmacokinetics; D001921:Brain / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015314:Dialysis Solutions / Q000032:analysis* / Q000737:chemistry; D005960:Glucosides / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D008297:Male; D017551:Microdialysis; D015394:Molecular Structure; D010636:Phenols / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D059808:Polyphenols
D000890:Anti-Infective Agents; D015314:Dialysis Solutions; D005960:Glucosides; D010636:Phenols; C058956:acteoside; D059808:Polyphenols